LOCUS       NP_001351746             538 aa            linear   INV 01-SEP-2025
DEFINITION  cytochrome P450 18a1 [Megachile rotundata].
ACCESSION   NP_001351746
VERSION     NP_001351746.1
DBSOURCE    REFSEQ: accession NM_001364817.1
KEYWORDS    RefSeq.
SOURCE      Megachile rotundata (alfalfa leafcutting bee)
  ORGANISM  Megachile rotundata
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae;
            Megachile.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AFJA01004398.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN03072610, SAMN03072611
                              [ECO:0006172]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Megachile rotundata"
                     /db_xref="taxon:143995"
                     /chromosome="6"
                     /map="6"
     Protein         1..538
                     /product="cytochrome P450 18a1"
                     /note="cytochrome P450 18a1-like"
                     /calculated_mol_wt=60568
     Region          79..509
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(134,138,145,197,318..319,322..323,326..327,330,381,
                     391,393,416,452..454,458..462,465..466)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(223..224,318,321..322,326,389..392)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..538
                     /gene="Cyp18a1"
                     /coded_by="NM_001364817.1:661..2277"
                     /db_xref="GeneID:100883331"
ORIGIN      
        1 mfvehaaqwa wramggtrie vlctllvfvg vllvarclqw lryvrslppg pwgvpvfgyl
       61 pflkgdvhlq ygelakkygp mfsarlgtql vvvlsdhrti rdtfrreeft grphtefini
      121 lggygiinte gamwkeqrkf lhdklrsfgm tymgggkkim esrimrevkt flrglaskrg
      181 tptdvsaslg msisnvicsl imgvrfqhgd drfkrfmdli eegfklfgsm aavnfipvmr
      241 ylpclqkirn kisenraema dffqktvdqh ratfdestvr dlvdayllei erakgegrat
      301 llfqgknhdr qmqqilgdlf sagmetvktt lewaiilmlh hpeaaaavqe eldqvvgrsr
      361 mpaledlpfl pvteatilev lrrssvvplg tthattrdvt lhgytipagt qvvpllhavh
      421 mdpelwekpn efrpsrflsa egkvekpeyf mpfgagrrmc lgdvlarmel flffsslmht
      481 felkspqgas lpslrgnagv tvtpdpfdvc llprnlniie dssndtifga ilrnigsh
//
LOCUS       XP_076549151            1735 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306171 [Osmia lignaria lignaria].
ACCESSION   XP_076549151
VERSION     XP_076549151.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693036.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478158) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 72% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1735
                     /product="uncharacterized protein LOC143306171"
                     /calculated_mol_wt=198984
     Region          138..277
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          467..606
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(472,474,476,497,499..500)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            472..474
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            472
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(497,499..501,505..508)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          737..952
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(801..806,841..842,877,879..880,943..944)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(801..806,841,879)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            842
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          968..1128
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1357..1406
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1449..>1504
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1635..1727
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1735
                     /gene="LOC143306171"
                     /coded_by="XM_076693036.1:385..5592"
                     /db_xref="GeneID:143306171"
ORIGIN      
        1 maqsenlktl iktrgslksn vtrlitylna hpdaeiddiq nrkikleegy eafsktqlqi
       61 eilaekekht dmdheahrsq fddqyfacak iiskrlrein svispiasts pstsatpsmp
      121 itikqannll pkieirpydg npinwysfhd tfknlvhdnd ellpvhkfyl lqnalqgplt
      181 sitenlnase enylvawntl kqrcnkprqi ihthlqllle lpeihretpt nlrslvektq
      241 vhinalralk qpvdkwdall iyivvkkldk gtrrawertl edeempafqk lldflskqar
      301 gddfdtisfd snksnshfnn htskmpnkkr pnqiqshvgt qahfqcpacs qnhaiytcnk
      361 flqlnpkerf elarraklcl nclrgnhqtn kcylsgcktc nhkhntllhf tdtnnprnhp
      421 tnnsiesndq nikeqptvnl vvssdsevll gtasikildk dkneipcrvl ldggsqthfi
      481 terladrlkl nktminlpfs glgqnltksq ytvkttiksr tspfscdltl valptitgll
      541 psrkidkdnl sipknislad pnfhksaeid allgntlfyq llsvgqiklc ndsiilqktr
      601 lgwivtgevn llnnhlnkar scfvttalen qlnkfwevee ipnkkyfsse esacethfsq
      661 nikrnldgry vvrlpfnekr dsigeshrma lkrfyslekr lelsselknq ysvflreyve
      721 lkhmtditnt ddkqvgyylp hhavikessa ttkvrvvfdg saktstsisl ndalmqgpti
      781 qddlisiiir frthqfvlta diekmfrqiq vhpddakfqk ilwrenktep iktymlntvt
      841 ygttsapfla vrclkqiikd dghkyplaat vldrdfyvdd lltgtndlnq acflidqltk
      901 llnlggfnlr qwlsnepkll nklknssasq lvcldvtetk kvlgiqwdph adtinykvnp
      961 fsshkvvskr tilseiaqlf dplgllgpvi iqaklvmqkl wmanlnwdes vpqsihtewf
     1021 nyknqlplln nftvhrkvvl nnaveiqlhg fcdasekgyg acfyirstnk vnnhlvdlvc
     1081 aksrvapikt islprlelca akllanlyks vvealnlrfn kinfysdsti alnwiktsph
     1141 ilktfeanrv seiqtltdrd nwyhvstndn padfisrgqn psdfikntlw vngptwlsme
     1201 ehcwkitkls tsdsanhqan vtfvtldqnc nrnkvandtf erfssinaln rfvalchrai
     1261 vnrsssnkit geittkelhd ahlqiirvvq esefsqelqc lrkgeelnnk srllrldpfi
     1321 delgilrvgg rlgnadinys hkhpillprn nhitdiiirh vhikywhtgv qntlaivrqn
     1381 ywpidgknrt ryvlhkcisc cklnhkppny pmgilpknrl kqsrpfenag ldycgpflik
     1441 ekkfrnkvki ksyvvvfvcf stkavhlelv tdlttetcla aikrffgrrg ksknlysdng
     1501 tnfvgaknei lkikalllss nhndrithdl snegvnwhfs ppqsphfggl weagvkqfky
     1561 hlyrtvgdsl ftyeqfntfi ieiesilnsr pltplssdpn dfsaltpahf ligdslmsip
     1621 ehdfqdvptn rlslwqhivk vkqhfwkrwh reyfnelllr skwhkgdpeq ikidtlvtik
     1681 ednippmhws mgriiavhpg edkiirvatv ktnrgiykrc ikklcplpie iperd
//
LOCUS       XP_076549152            1237 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611286 [Osmia lignaria lignaria].
ACCESSION   XP_076549152
VERSION     XP_076549152.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693037.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478158) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1237
                     /product="uncharacterized protein LOC117611286"
                     /calculated_mol_wt=139442
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          936..987
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1217..>1237
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1237
                     /gene="LOC117611286"
                     /coded_by="XM_076693037.1:1..3714"
                     /db_xref="GeneID:117611286"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qarayssfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifatditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrrt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyegkhpa ilprhsrlse livdhshkat lhggtqltla
      961 hirqtywiig grapvkshil rcvvcarqrg irahqlmgql plsrvtpshp fshtgvdyag
     1021 pialktwkgr gaktqkgwic vfvcmttsav hlemvtdyst dgfvatyrrf aarrgiphti
     1081 ysdcgtnfig adsalksmfv egtqdnqrla klfvddhttw sfnppaaphm ggkweavvks
     1141 vkyhlrrtvs dtlltfeeyy tllnqieatl nsrplepltd dpddlsvltp ahfligrais
     1201 tlpepsveqa nisprarwhl iqqklqqfwk rwstqhl
//
LOCUS       XP_034186988            1323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607426 [Osmia lignaria lignaria].
ACCESSION   XP_034186988
VERSION     XP_034186988.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331097.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478158) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186988.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="uncharacterized protein LOC117607426"
                     /calculated_mol_wt=148860
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          936..987
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1217..1309
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1323
                     /gene="LOC117607426"
                     /coded_by="XM_034331097.2:1..3972"
                     /db_xref="GeneID:117607426"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qarayssfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifstditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrrt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyegkhpa ilprhsrlse livdhshkat lhggtqltla
      961 hirqtywiig grapvkshil rcvvcarqrg irahqlmgql plsrvtpshp fshtgvdyag
     1021 pialktwkgr gaktqkgwic vfvcmttsav hlemvtdyst dgfvatyrrf aarrgiphti
     1081 ysdcgtnfig adsalksmfv egtqdnqrla klfvddhttw sfnppaaphm ggkweavvks
     1141 vkyhlrrtvs dtlltfeeyy tllnqieatl nsrplepltd dpddlsvltp ahfligrais
     1201 tlpepsveqa nisprarwhl iqqklqqfwk rwstqhlqrm qsiskwhhps nnihvgslvl
     1261 ltderfppck wplarvlavh pgpdgltriv tiktatstlt rpiaklallp vedqdsspss
     1321 tsc
//
LOCUS       XP_034194536            1209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610816 [Osmia lignaria lignaria].
ACCESSION   XP_034194536
VERSION     XP_034194536.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338645.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478158) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194536.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1209
                     /product="uncharacterized protein LOC117610816"
                     /calculated_mol_wt=138468
     Region          <2..77
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          207..421
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(271..276,310..311,346,348..349,412..413)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(271..276,310,348)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            311
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          437..597
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          814..864
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          909..1003
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1093..1184
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1209
                     /gene="LOC117610816"
                     /coded_by="XM_034338645.2:1..3630"
                     /db_xref="GeneID:117610816"
ORIGIN      
        1 mipkiaslip dqqidrsnik ipknlnladp efhrpapidl llgsgtalsl vsvgqinlsp
       61 pdgpdlylqk trlgwviggs ppqpiksriv scnattalqh dlarfweiee gpqlqllses
      121 dkfcekhfqq hvkrnsdgry vvalpfndkl lllgesrara lkrleslerk lqrdatlrge
      181 yhaviqeyld lghmtkvtni qcpsdgyylp hhgvtkitse ttklrvvfdg saatssgisl
      241 ndalhigpri qedllyillr frvhryvitg diekmyrqfl vrdedqkyqr vlwrdasrei
      301 qtyelrtvtf glsaapylai rcltqlaqde chrfpraaqi lrrdfyvddl ltgastlqea
      361 aslredltql lklaslnirq wasnhedllr nlpeesinrk irigesstlk tlgiiwdsan
      421 dsisyevktq iansyvtkrf itseiakiyd plgligpvii vakmllqriw tikvdwdesl
      481 pmdihtewkq fhrqlpllns tvfrrrtvid tpsrielhgf cdasekayga ciyirssdmh
      541 dnttvellla kskvaplktq siprlelcga lllttlyntv kkalhvklnr tifwtdstvv
      601 lhwlqtspht lktfvanris eiqartnigd wrhvpttdnp adlisrgqhp eeflqpsiwh
      661 hgpewlkhee vtwptrdlip cnfipeqrta tclkvspldt nilddysswe kmqrivawcl
      721 rwkgdnmikg pltpvelrha rdviikllqr ihfskelrcl ssssnpeiag klqrlspfvd
      781 kdgivrvggr lkhssmpftq rhpiilpkar vtgliieaeh raqlhagvqg tlyavrrrfw
      841 pidgrnqvwk alrtctrclr arppatnyim gelpearvte arpftnvgvd ycgpfyiker
      901 rhrnrtrikv yvavfvclav kavhlelvsd ltseafiaal rrfiarrgyc vkihsdngtn
      961 fvganneiqe iqrlirsdeh ntkvqnflaq rsiewsfipp raphfgglwe aavksfknhl
     1021 lrvagtelft fedfntliie iesilnsrpl tpmssdpndl lvltpghfli gdsltslrer
     1081 dyldtppnrl sswqfiqrik qhfwtrwhre ylneltrrtk wskgehpike gtlvllredn
     1141 vppmqwalgr vlrtypgsdg iirtvtikta aseldrsvkr lvplpyqvee rsnepeepas
     1201 tanvnpidd
//
LOCUS       XP_076549154             300 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306172 [Osmia lignaria lignaria].
ACCESSION   XP_076549154
VERSION     XP_076549154.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693039.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478158) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="uncharacterized protein LOC143306172"
                     /calculated_mol_wt=34653
     Region          <1..126
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(15,51,124)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          <230..>298
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..300
                     /gene="LOC143306172"
                     /coded_by="XM_076693039.1:1..903"
                     /db_xref="GeneID:143306172"
ORIGIN      
        1 mvainynnfl iiseyispni dvvtvderld eiseviksnk wsgillagdf nskspvwggn
       61 twnargkill eyvlseslsp iytvggntci kgngskidll itstnlcykv ttsevldeft
      121 gsdhlylwhe vnlenpeidk tksirnknkk tknrgnkrmn rstidsnkyv nkflekygdr
      181 ennrysinqt vndikkvled mqkigsnctk rnplrpgrcp vhwwtpeiae qrkatnklrr
      241 kvtrlrrkke kelselaere fklakkelnk kirnsrketv ekliktidnd mwgealqndn
//
LOCUS       XP_076549155             561 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306173 [Osmia lignaria lignaria].
ACCESSION   XP_076549155
VERSION     XP_076549155.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693040.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478158) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="uncharacterized protein LOC143306173"
                     /calculated_mol_wt=62223
     Region          6..204
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(10,12,37,93,126,128,202)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(10,37,93,126,128,176,201..202)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(37,201)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          <412..>479
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..561
                     /gene="LOC143306173"
                     /coded_by="XM_076693040.1:290..1975"
                     /db_xref="GeneID:143306173"
ORIGIN      
        1 msaftvlqan lnhcraaqdi lwqaahdyra divvisephr prpewhtdps gtlsmwcvqr
       61 tpptaelgmi rkihtsdgci gletrdfvif gcyfspsltm atyegqldgl aagvasstkp
      121 viisgdfnar svqwgsdrtd argyrllrvc eqsglipvns kggpsfvrnh qqsridavac
      181 shalrsrlks sfvstkytgs dhnylvhrfs atdtfesssm nsmwntsynp pdkeawasii
      241 knnlslppet dawvrelpih vfedtasidt aiaeleswye sariprshns arkrkyvaww
      301 tkeisrlrln aarsrakwrr aalnmsknnr nvsvdsqhpl rqlmvsdrra likairqskd
      361 aswsgmidei arypwgkpyk avmnrlkgrs pllrlnetef ktavsqlfvt eppvttagsa
      421 pvpdvtnptp aprnrsaspq pgpscttpsr stsigpaapp svnrpitnst sptpsftepi
      481 crvapedpri pypfstrdav aalakvsggk apgrdgitge alrpimrlap ewirdlfnac
      541 yrlgyfpnsw kvgrlvlipk t
//
LOCUS       XP_076549156            1736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306192 [Osmia lignaria lignaria].
ACCESSION   XP_076549156
VERSION     XP_076549156.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693041.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478159) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1736
                     /product="uncharacterized protein LOC143306192"
                     /calculated_mol_wt=197327
     Region          136..276
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          441..592
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          725..940
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(789..794,829..830,865,867..868,931..932)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(789..794,829,867)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            830
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          956..1116
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1336..1392
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1434..1531
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1621..1713
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1736
                     /gene="LOC143306192"
                     /coded_by="XM_076693041.1:1..5211"
                     /db_xref="GeneID:143306192"
ORIGIN      
        1 maeqisrlkv kianakrkit nvakfvegyv aerdypvlek rkqdidkeft efenwytele
       61 alvedpthad irteyeekyy aaygklmlfl kdvaapinnl adpiasthsq sivngvntni
      121 vqtskrslpr vnlpsfsgsy eawlgfhdlf kslvdddqni peieklyhlk gclrdeaaev
      181 lasielsaen yrvawsllke rydnrklirq thvkallnlk hvskefpvrs lldqvqkhvr
      241 aldalkepvd hwntllveii sqklnnvire kweefsgesv sptfkelitf lqrraqfedv
      301 kshqdfvgaq nlsdrkpsha hvkqrsqhay ptatanhscs hcrgkhsihn cetfkklsps
      361 arfeaakkat lcinclrpnh rvmdctgapc rkchkkhntl lhfekqppea tsasepvsss
      421 qsvvnlhaqv ssegllatam vdivnaqgks ktcrafldag sqahfitesv asflrlprks
      481 vdisvsgved isteikhsvs ailksrfsky sktleflvln ritkpmpsia vnqtnldipn
      541 nvvladpkfy dpsqievlig vklfykllcv gqislknhpe avlqktqlgw ivageingss
      601 srnavqchft thstpldatl tkfwelenip svkvlsqeeq aceahfksnt qrnsegryiv
      661 rlpfnenken lgesrsmalr rfhflekrfe knpqlkveyc hfldeykqln hlsllenktl
      721 tmpgfylphh aviredsitt kirvvfdgsa rtssgvslnn almvgptiqd dlftlltrfr
      781 srryaltadi ekmyrqiqvh pddtpyqkil frknpdetvn elsldtvtyg tscapflair
      841 tlhqlandeg aqhpvaasvl kqdfyvddll tgagtrqeaa flrddliall qkggfpirkw
      901 asndpslvpe nsdnsssthm sldpnatikt lgihwnsred iifykvntpn lvktvtkrsi
      961 lsqvaklfdp lgllgpiivk akimiqllwk agvswdesvp adihtmwlef keqlpllvkv
     1021 sfnrltiapn fvkiqmhgfs dasekaygac iymrstdihg nhhvslvcsk srvapvkslt
     1081 lprlelcaal llarlyttvk lalqleinkv ylwsdstitl hwintephll ktfvsnriae
     1141 iqnlsescew rhvptqdnpa dlvsrgqmpq efvdtriwkn gphwlsheen fwpqkkihlv
     1201 eipekraiia apsfananii dddlikdyss lkslkrviay vmrfihnlrn ksqkhtgpis
     1261 atefeastri lvlitqsiaf areiyslkid grvdnksrli plkpfldkqg ilrvggrlih
     1321 selpkeqkhq iilpsdhhit rliireeher lnhagtqatl ysvretfwpl ngrnvtriii
     1381 hkcvrcfrak prsvdyimgd lpqervscsr pfldvgvdyc gplyikekrf rnrnkikvyv
     1441 aifvcmstka vhlelvsdlt teafmaclkr lfsrrgisrt ihsdnatnfv gagrelialh
     1501 kliqsdehnt aiqkflaeqk vtwnfipprs phfgglweaa vrsfkhhlvr tvgdtlltfe
     1561 qletciieie ailnsrplsp mspdpndlqp ltpghfligg pltsfpemdl tdtasnrlsa
     1621 wqhaqklrqh fwrrwqkeyl hqltirskwq snlnhcihpg tlvvikednl pplrwklgri
     1681 vathpgpdgi trvatiktdt geckrcvkkl cplpiecssk adentklcsh nstsnt
//
LOCUS       XP_076549157            1736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306193 [Osmia lignaria lignaria].
ACCESSION   XP_076549157
VERSION     XP_076549157.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693042.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478159) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1736
                     /product="uncharacterized protein LOC143306193"
                     /calculated_mol_wt=197357
     Region          136..276
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          441..592
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          725..940
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(789..794,829..830,865,867..868,931..932)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(789..794,829,867)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            830
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          956..1116
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1336..1392
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1434..1531
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1621..1713
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1736
                     /gene="LOC143306193"
                     /coded_by="XM_076693042.1:1..5211"
                     /db_xref="GeneID:143306193"
ORIGIN      
        1 maeqisrlkv kianakrkit nvakfvegyv aerdypvlek rkqdidkeft efenwytele
       61 alvedpthad irteyeekyy aaygklmlfl kdvaapinnl adpiasthsq sivngvntni
      121 vqtskrslpr vnlpsfsgsy eawlgfhdlf kslvdddqni peieklyhlk gclrdeaaev
      181 lasielsaen yrvawsllke rydnrkiirq thvkallnlk hvskefpvrs lldqvqkhvr
      241 aldalkepvd hwntllveii sqklnnvire kweefsgesv sptfkelitf lqrraqfedv
      301 kshqdfvgaq nlsdrkpsha hvkqrsqhay ptatanhscs hcrgkhsihn cetfkklsps
      361 arfeaakkat lcinclrpnh rvmdctgapc rkchkkhntl lhfekqppea tsasepvsss
      421 qsvvnlhaqv ssegllatam vdivnaqgks ktcrafldag sqahfitesv asflrlprks
      481 vdisvsgved isteikhsvs ailksrfsky sktleflvln ritkpmpsia vnqtnldipn
      541 nvvladpkfy dpsqievlig vklfykllcv gqislknhpe avlqktqlgw ivageingss
      601 srnavqchft thstpldatl tkfwelenip svkvlsqeeq aceahfksnt qrnsegryiv
      661 rlpfnenken lgesrsmalr rfhflekrfe knpqlkveyc hfldeykqln hlsllenktl
      721 tmpgfylphh aviredsitt kirvvfdgsa rtssgvslnn almvgptiqd dlftlltrfr
      781 srryaltadi ekmyrqiqvh pddtpyqkil frknpdetvn elsldtvtyg tscapflair
      841 tlhqlandeg aqhpvaasvl kqdfyvddll tgagtrqeaa flrddliall qkggfpirkw
      901 asndpslvpe nsdnsssthm sldpnatikt lgihwnsred iifykvntpn lvktvtkrsi
      961 lsqvaklfdp lgllgpiivk akimiqllwk agvswdesvp adihtmwlef keqlpllvkv
     1021 sfnrltiapn fvkiqmhgfs dasekaygac iymrstdihg nhhvslvcsk srvapvkslt
     1081 lprlelcaal llarlyttvk lalqleinkv ylwsdstitl hwintephll ktfvsnriae
     1141 iqnlsescew rhvptqdnpa dlvsrgqmpq efvdtriwkn gphwlsheen fwpqkkihlv
     1201 eipekraiia apsfananii dddlikdyss lkslkrviay vmrfihnlrn ksqkhtgpis
     1261 atefeastri lvlitqsiaf areiyslkid grvdnksrli plkpfldkqg ilrvggrlih
     1321 selpkeqkhq iilpsdhhit rliireeher lnhagtqatl ysvretfwpl ngrnvtriii
     1381 hkcvrcfrak prsvdyimgd lpqervscsr pfldvgvdyc gplyikekrf rnrnkikvyv
     1441 aifvcmstka vhlelvsdlt teafmaclkr lfsrrgisrt ihsdnatnfv gasrelialh
     1501 kliqsdehnt aiqkflaeqk vtwnfipprs phfgglweaa vrsfkhhlvr tvgdtlltfe
     1561 qletciieie ailnsrplsp mspdpndlqp ltpghfligg pltsfpemdl tdtasnrlsa
     1621 wqhaqklrqh fwrrwqkeyl hqltirskwq snlnhcihpg tlvvikednl pplrwklgri
     1681 vathpgpdgi trvatiktdt geckrcvkkl cplpiecssk adentklcsh nstsnt
//
LOCUS       XP_076549158            1736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306194 [Osmia lignaria lignaria].
ACCESSION   XP_076549158
VERSION     XP_076549158.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693043.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478159) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1736
                     /product="uncharacterized protein LOC143306194"
                     /calculated_mol_wt=197329
     Region          136..276
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          441..592
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          725..940
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(789..794,829..830,865,867..868,931..932)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(789..794,829,867)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            830
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          956..1116
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1336..1392
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1434..1531
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1621..1713
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1736
                     /gene="LOC143306194"
                     /coded_by="XM_076693043.1:1..5211"
                     /db_xref="GeneID:143306194"
ORIGIN      
        1 maeqisrlkv kianakrkit nvakfvegyv aerdypvlek rkqdidkeft efenwytele
       61 alvedpthad irteyeekyy aaygklmlfl kdvaapinnl adpiasthsq sivngvntni
      121 vqtskrslpr vnlpsfsgsy eawlgfhdlf kslvdddqni peieklyhlk gclrdeaaev
      181 lasielsaen yrvawsllke rydnrkiirq thvkallnlk hvskefpvrs lldqvqkhvr
      241 aldalkepvd hwntllveii sqklnnvire kweefsgesv sptfkelitf lqrraqfedv
      301 kshqdfvgaq nlsdrkpsha hvkqrsqhay ptatanhscs hcrgkhsihn cetfkklsps
      361 arfeaakkat lcinclrpnh rvmdctgapc rkchkkhntl lhfekqppea tsasepvsss
      421 qsvvnlhaqv ssegllatam vdivnaqgks ktcrafldag sqahfitesv asflrlprks
      481 vdisvsgved isteikhsvs ailksrfsky sktleflvln ritkpmpsia vnqtnldipn
      541 nvvladpkfy dpsqievlig vklfykllcv gqislknhpe avlqktqlgw ivageingss
      601 srnavqchft thstpldatl tkfwelenip svkvlsqeeq aceahfksnt qrnsegryiv
      661 rlpfnenken lgesrsmalr rfhflekrfe knpqlkveyc hfldeykqln hlsllenktl
      721 tmpgfylphh aviredsitt kirvvfdgsa rtssgvslnn almvgptiqd dlftlltrfr
      781 srryaltadi ekmyrqiqvh pddtpyqkil frknpdetvn elsldtvtyg tscapflair
      841 tlhqlandeg aqhpvaasvl kqdfyvddll tgagtrqeaa flrddliall qkggfpirkw
      901 asndpslvpe nsdnsssthm sldpnatikt lgihwnsred iifykvntpn lvktvtkrsi
      961 lsqvaklfdp lgllgpiivk akimiqllwk agvswdesvp adihtmwlef keqlpllvkv
     1021 sfnrltiapn fvkiqmhgfs dasekaygac iymrstdihg nhhvslvcsk srvapvkslt
     1081 lprlelcaal llarlyttvk lalqleinkv ylwsdstitl hwintephll ktfvsnriae
     1141 iqnlsescew rhvptqdnpa dlvsrgqmpq efvdtriwkn gphwlsheen fwpqkkihlv
     1201 eipekraiia apsfananii dddlikdyss lkslkrviay vmrfihnlrn ksqkhtgpis
     1261 atefeastri lvlitqsiaf areiyslkid grvdnksrli plkpfldkqg ilrvggrlih
     1321 selpkeqkhq iilpsdhhit rliireeher lnhagtqatl ysvretfwpl ngrnvtriii
     1381 hkcvrcfrak prsvdyimgd lpqervscsr pfldvgvdyc gplyikekrf rnrnkikvyv
     1441 aifvcmstka ahlelvsdlt teafmaclkr lfsrrgisrt ihsdnatnfv gasrelialh
     1501 kliqsdehnt aiqkflaeqk vtwnfipprs phfgglweaa vrsfkhhlvr tvgdtlltfe
     1561 qletciieie ailnsrplsp mspdpndlqp ltpghfligg pltsfpemdl tdtasnrlsa
     1621 wqhaqklrqh fwrrwqkeyl hqltirskwq snlnhcihpg tlvvikednl pplrwklgri
     1681 vathpgpdgi trvatiktdt geckrcvkkl cplpiecssk adentklcsh nstsnt
//
LOCUS       XP_034194199            1323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610672 [Osmia lignaria lignaria].
ACCESSION   XP_034194199
VERSION     XP_034194199.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338308.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478159) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194199.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="uncharacterized protein LOC117610672"
                     /calculated_mol_wt=148830
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          936..987
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1217..1309
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1323
                     /gene="LOC117610672"
                     /coded_by="XM_034338308.2:1..3972"
                     /db_xref="GeneID:117610672"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qarayssfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifatditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrrt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyegkhpa ilprhsrlse livdhshkat lhggtqltla
      961 hirqtywiig grapvkshil rcvvcarqrg irahqlmgql plsrvtpshp fshtgvdyag
     1021 pialktwkgr gaktqkgwic vfvcmttsav hlemvtdyst dgfvatyrrf aarrgiphti
     1081 ysdcgtnfig adsalksmfv egtqdnqrla klfvddhttr sfnppaaphm ggkweavvks
     1141 vkyhlrrtvs dtlltfeeyy tllnqieatl nsrplepltd dpddlsvltp ahfligrtis
     1201 tlpepsveqa nisprarwhl iqqklqqfwk rwstqhlqrm qsiskwhhps nnihvgslvl
     1261 ltderfppck wplarvlavh pgpdgltrvv tiktatstlt rpiaklallp vedqdsspss
     1321 tsc
//
LOCUS       XP_076549161             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306225 [Osmia lignaria lignaria].
ACCESSION   XP_076549161
VERSION     XP_076549161.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693046.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478160) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC143306225"
                     /calculated_mol_wt=16896
     CDS             1..150
                     /gene="LOC143306225"
                     /coded_by="XM_076693046.1:503..955"
                     /db_xref="GeneID:143306225"
ORIGIN      
        1 mcyireqpte rdtsriellm nltarlcmrs tcyrldvgiv cmqcaleaal gaariydmve
       61 vhyplptayp pgacdicsap lmesqaayhc eeciaaiime meyqranqpi pppaivptwy
      121 terqirsrqp gprrtlrlht avssitnhht
//
LOCUS       XP_076549162             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306226 [Osmia lignaria lignaria].
ACCESSION   XP_076549162
VERSION     XP_076549162.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693047.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478160) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC143306226"
                     /calculated_mol_wt=16896
     CDS             1..150
                     /gene="LOC143306226"
                     /coded_by="XM_076693047.1:503..955"
                     /db_xref="GeneID:143306226"
ORIGIN      
        1 mcyireqpte rdtsriellm nltarlcmrs tcyrldvgiv cmqcaleaal gaariydmve
       61 vhyplptayp pgacdicsap lmesqaayhc eeciaaiime meyqranqpi pppaivptwy
      121 terqirsrqp gprrtlrlht avssitnhht
//
LOCUS       XP_034195140            1323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611301 [Osmia lignaria lignaria].
ACCESSION   XP_034195140
VERSION     XP_034195140.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339249.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478162) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195140.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="uncharacterized protein LOC117611301"
                     /calculated_mol_wt=148829
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          936..987
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1217..1309
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1323
                     /gene="LOC117611301"
                     /coded_by="XM_034339249.2:1..3972"
                     /db_xref="GeneID:117611301"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qarayssfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifatditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrrt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyegkhpa ilprhsrlse livdhshkat lhggtqltla
      961 hirqtywiig grapvkshil rcvvcarqrg irahqlmgql plsrvtpshp fshtgvdyag
     1021 pialktwkgr gaktqkgwic vfvcmttsav hlemvtnyst dgfvatyrrf aarrgiphti
     1081 ysdcgtnfig adsalksmfv egtqdnqrla klfvddhttw sfnppaaphm ggkweavvks
     1141 vkyhlrrtvs dtlltfeeyy tllnqieatl nsrplepltd dpddlsvltp ahfligrais
     1201 tlpepsveqa nisprarwhl iqqklqqfwk rwstqhlqrm qsiskwhhps nnihvgslvl
     1261 ltderfppck wplarvlavh pgpdgltrvv tiktatstlt rpiaklallp vedqdsspss
     1321 tsc
//
LOCUS       XP_076549163             597 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306261 [Osmia lignaria lignaria].
ACCESSION   XP_076549163
VERSION     XP_076549163.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693048.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478162) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="uncharacterized protein LOC143306261"
                     /calculated_mol_wt=66913
     Region          <512..564
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..597
                     /gene="LOC143306261"
                     /coded_by="XM_076693048.1:3391..5184"
                     /db_xref="GeneID:143306261"
ORIGIN      
        1 mdvyfrparg tlmesfikpn nkniksqknk tgqeqyttti gkkedmikpn drkvrddvyk
       61 vivgegteki ervkitddik lnktnkvfdf ksafrptrtl srsptrksge itadvdltgd
      121 edenvfsdts samgnmpktd dsnkqkeset ekesknvggl naalmaidqi lgmvkssndl
      181 vnnyllkiae gssmvaqiss dmgvnliktr dlikeaiisy keekskqeey tkreekrtiq
      241 ilesldkigk rqnildtrne itenlnkrkn vtppemeati krtrttnket rskpmirsiq
      301 iiknsegass dsdwetvgtk rrkqpnekik nlntgnnttp yndtnrkrsi ttavkrneai
      361 aisvnkemty aqatkklkek mgnnvegvkr irhtrtgdil iefdretdst dfhnkvksvm
      421 gedakvrrlv pkvtievldi dpgadkdeiv ecisistgve kdsikcknir ssfagtqtai
      481 iegpggigkl lienrikigw vycrvrllps iircfkchnf ghlatrcaav egeavvcrrc
      541 genshamsec kaekascrlc aqkgivgsel nhiagslrcr qykeaisgai rvnkiqs
//
LOCUS       XP_076549164            1323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306279 [Osmia lignaria lignaria].
ACCESSION   XP_076549164
VERSION     XP_076549164.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693049.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478163) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="uncharacterized protein LOC143306279"
                     /calculated_mol_wt=148802
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          936..987
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1217..1309
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1323
                     /gene="LOC143306279"
                     /coded_by="XM_076693049.1:1..3972"
                     /db_xref="GeneID:143306279"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qarayssfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifatditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrkt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyegkhpa ilprhsrlse livdhshkat lhggtqltla
      961 hirqtywiig grapvkshil rcvvcarqrg irahqlmgql plsrvtpshp fshtgvdyag
     1021 pialktwkgr gaktqkgwic vfvcmttsav hlemvtdyst dgfvatyrrf aarrgiphti
     1081 ysdcgtnfig adsalksmfv egtqdnqrla klfvddhttw sfnppaaphm ggkweavvks
     1141 vkyhlrrtvs dtlltfeeyy tllnqieatl nsrplepltd dpddlsvltp ahfligrais
     1201 tlpepsveqa nisprarwhl iqqklqqfwk rwstqhlqrm qsiskwhhps nnihvgslvl
     1261 ltderfppck wplarvlavh pgpdgltrvv tiktatstlt rpiaklallp vedqdsspss
     1321 tsc
//
LOCUS       XP_076549166            1760 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306369 [Osmia lignaria lignaria].
ACCESSION   XP_076549166
VERSION     XP_076549166.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693051.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478164) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1760
                     /product="uncharacterized protein LOC143306369"
                     /calculated_mol_wt=197248
     Region          134..275
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          473..625
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(490,492,494,517..519)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            490..492
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            490
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(517..520,524..527)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          767..984
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(832..837,872..873,908,910..911,980..981)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(832..837,872,910)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            873
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          1001..1161
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1377..1430
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1655..1748
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1760
                     /gene="LOC143306369"
                     /coded_by="XM_076693051.1:1..5283"
                     /db_xref="GeneID:143306369"
ORIGIN      
        1 mpsttlkrli avqnahlaei nsmgtrfrek arstlnkniv qarldaiigi waearkthve
       61 ivtrddansd pyvidnvfar mqcayedtld yfltlktece aaagstqair tvegnasgld
      121 lrpaklpkld lptfsgryed wdsfcdlftt lvhdaprvad atklqylksc lkgvaadlvk
      181 dvastsanya stwlalrarf hnprltvtnh lnallkmnsl kresadelrs fadeaqrivr
      241 alrnlqmpve hwdiwfvhvl asrldpesrk dweselsard reviartdtg akepdplarf
      301 pkfselaefl erraqalsmm sagdckrant mfkptpvsrk ifhanalqrt nskvasfsct
      361 lcsgphfisk cpkflaksss drraevrrly lcfncmgghk fkscpssgrc rtcqgkhhsl
      421 lhydpsqtsq pstsgleikl rdekpaptvp gvsahtaslp airypvilat aqvnvvgpqg
      481 thtrvralld qgaeasfvse aivqllalpk qrvqipltgl grsaagtarf avkftmqslv
      541 nsefkieaea lvlsrltsql ptrslieidl eqfgelslad phfnvpdsvd lilgadlygq
      601 llrpglqhlt gsrlvaqntv lgwvisgsaq ssssrraegs sqeplralhc aseddlnecl
      661 qqfwrleelp ssvrilgped eacenqfvat hsrdaggryv vqlplksdpp kvsaetrrma
      721 vgslahihrk ftrdpelasa yrefmrtyea lghmtrvpaa esinprswyl phhavvqstp
      781 tqykvrvvfd asrrtheqyr lndflmsgpp lqqdlslvll nwrrykyvft adivkmfrqi
      841 lvvprdqdlq rivwapnpee ppieyrlttv tygtacapyl airtlaqlav deksrfplga
      901 rclesnvyvd dmfigadelp lairkrteli qilgtagiel dkwaanhpdi lpahkrpitd
      961 dlvrdivqde siktlgvhwk ptqdefsfsv lkangllgvl tkravlsnia rlfdplgwls
     1021 pititakilm qdlwilkcdw dsplpaearg rwidycrsls slptltisrw fgspsesagq
     1081 ihgfsdassr ayaaviylrt degdghvrvs llaaktrvap vktvsipnle lcgalllvrl
     1141 vchlrkldfl krhpvflwsd srvvlawlnk hpchwktfva nrvsliqtel psatwayvpt
     1201 kqnpadcatr gsrpselhes slwwygpswl nlqpehwpqp setaqvlhae vkppepdils
     1261 rhstltrlir vvayclrpvk hlrrrkggln plpaylttse lsdarstiir laqahafase
     1321 ierlksgkpl pkssalrrln plldkedgil rvggrlswsn ltserkhppi lprhsglsrl
     1381 fvryahhaal hggptltssv llryawvvgg kslvktairn cltcqrvkpr lahqlmgdlp
     1441 aarvtparpf tttgldyagp fhirttkgrg hraykgyial fvcfatraih lelvsdltas
     1501 tfiaayrrfv srrgichrly sdnattfkga dtelrtmfee asefyqavas tlandgtaws
     1561 fippttphyg glweagvksv khhlrrvvge htltfeefst vlteieacln srplcpltsd
     1621 tddlnvltpa hfligtasal vpdaepssap enrlnrfqll qrirnqfwkr wsdeylqhlq
     1681 ergkwrdpta nfavgqlvlm rderyppakw hlgrvievhp gsdglvrvvt vktatntfrr
     1741 hiarlcplil eseathrlpk
//
LOCUS       XP_076549167            1760 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306370 [Osmia lignaria lignaria].
ACCESSION   XP_076549167
VERSION     XP_076549167.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693052.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478164) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1760
                     /product="uncharacterized protein LOC143306370"
                     /calculated_mol_wt=197107
     Region          134..275
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          473..625
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(490,492,494,517..519)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            490..492
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            490
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(517..520,524..527)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          767..984
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(832..837,872..873,908,910..911,980..981)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(832..837,872,910)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            873
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          1001..1161
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1377..1430
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1655..1748
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1760
                     /gene="LOC143306370"
                     /coded_by="XM_076693052.1:1..5283"
                     /db_xref="GeneID:143306370"
ORIGIN      
        1 mpsttlkrli avqnahlaei nsmgtrfrek arstlnkniv qarldaiigi waearkthve
       61 ivtrddansd pyvidnvfar mqcayedtld yfltlktece aaagstqair tvegnasgld
      121 lrpaklpkld lptfsgryed wdsfcdlftt lvhdaprvad atklqylksc lkgvaadlvk
      181 dvastsanya stwlalrarf hnprltvtnh lnallkmnsl kresadelrs fadeaqrivr
      241 alrnlqmpve hwdiwfvhvl asrldpesrk dweselsard reviartdtg akepdplarf
      301 pkfselaefl erraqalsmm sagdckrant mfkptpvsrk ifhanalqrt nskvasfsct
      361 lcsgphfisk cpkflaksss drraevrrly lcfncmgghk fkscpssgrc rtcqgkhhsl
      421 lhydpsqtsq pstsgleikl rdekpaptvp gvsahtaslp airypvilat aqvnvvgpqg
      481 thtrvralld qgaeasfvse aivqllalpk qrvqipltgl gssaagtarf avkftmqslv
      541 nsefkieaea lvlsrltsql ptrslieidl eqfgelslad phfnvpdsvd lilgadlygq
      601 llrpglqhlt gsrlvaqntv lgwvisgsaq ssssrraegs sqeplralhc aseddlnecl
      661 qqfwrleelp ssvrilgped eacenqfvat hsrdaggryv vqlplksdpp kvsaetrrma
      721 vgslahihrk ftrdpelasa yrefmrtyea lghmtrvpaa esinprswyl phhavvqstp
      781 tqykvrvvfd asrrtheqyr lndflmsgpp lqqdlslvll nwrrykyvft adivkmfrqi
      841 lvvprdqdlq rivwapnpee ppieyrlttv tygtacapyl airtlaqlav deksrfplga
      901 rclesnvyvd dmfigadelp lairkrteli qilgtagiel dkwaanhpdi lpahkrpitd
      961 dlvrdivqde siktlgvhwk ptqdefsfsv lkangllgvl tkravlsnia rlfdplgwls
     1021 pititakilm qdlwilkcdw dsplpaearg rwidycrsls slptltisrw fgspsesagq
     1081 ihgfsdassr ayaaviylrt degdghvrvs llaaktrvap vktvsipnle lcgalllvrl
     1141 vchlrkldfl krhpvflwsd srvvlawlnk hpchwktfva nrvsliqtel psatwayvpt
     1201 kqnpadcatr gsrpselhes slwwygpswl nlqpehwpqp setaqvlhae vkppepdils
     1261 rhstltrlir vvayclrpvk hlrrrkggln plpaylttse lsdarstiir laqahafase
     1321 ierlksgkpl pkssalrrln plldkedgil rvggrlswsn ltserkhppi lprhsglsrl
     1381 fvryahhaal hggptltssv llryawvvgg kslvktairn cltcqrvkpr lahqlmgdlp
     1441 aarvtparpf tttgldyagp fhirttkgrg hraykgyial fvcfatraih lelvsdltas
     1501 tfiaayrrfv srrgichrly sdnattfkga dtelrtmfee asefyqavas tlandgtaws
     1561 fippttphyg glweagvksv khhlrrvvge htltfeefst vlteigacln srplcpltsd
     1621 tddlnvltpa hfligtasal vpdaepssap enrlnrfqll qrirnqfwkr wsdeylqhlq
     1681 ergkwrdpta nfavgqlvlm rderyppakw hlgrvievhp gsdglvrvvt vktatntfrr
     1741 hiarlcplil eseathrlpk
//
LOCUS       XP_076549168            1301 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306442 [Osmia lignaria lignaria].
ACCESSION   XP_076549168
VERSION     XP_076549168.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693053.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478165) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1301
                     /product="uncharacterized protein LOC143306442"
                     /calculated_mol_wt=144985
     Region          136..275
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          458..609
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          747..963
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(810..815,850..851,886,888..889,954..955)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(810..815,850,888)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            851
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          978..1139
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..1301
                     /gene="LOC143306442"
                     /coded_by="XM_076693053.1:1..3906"
                     /db_xref="GeneID:143306442"
ORIGIN      
        1 mgdlktllhq qteaarvver tldnfkkmgk nkwtvavvrn rlerlsesfa ecktlhkeis
       61 aaatdaeras ldyftkerfm vlheiyldts dfmaeilskl eaagssgtst trpadeavhq
      121 ssnvvdrlpp lhlpqfsgsi sewesfrdrf samiiarqgl snvsrfqyll sclqgeaadl
      181 vtniaiteag fqlvwdrlvg ryentrllvt thvntlydlp qvrsesaeql rhlrdkanma
      241 vnalknlkrp vdtwddllvf fvtkkldsas rkawelqlgn dtqcpkfsvl dtflqsrira
      301 lqsigsstsg starnaemst tksnrsraas lhgaaiakgt celcksnhtl gqcssfktks
      361 aiqrfefvkk anrcvnclsp nhatrkctsl hgctickkrh htllhfdest astnettssq
      421 ngassssnad dpatttftsc svpritatqq vllatariel rspsgrseki rvlldqgsvv
      481 siisenltqr lriprsrvsa citgigntai scrslatfti ascktesvys mtayvlrsls
      541 sytpnrvtad vklshirdld ladenpfssd piemiigadq yglflldglr kgttkeptaq
      601 nttlgwilsg pvpssssiss astanfhcnv ssnldielrr fweieelpsv trlspseqqc
      661 eehfqrshtr apngqyivrl pfvegppidi gpslsvaitm flrqenrlrr naehltefsk
      721 flheyealgh mvevtdsssv ssaqtvyipy havirehstt trlrvvfnas aktangksln
      781 dhlligpklq idlpavmlrw rlyqyvfiad iakmyrqilv nskdvdyqri lwrsspsdpl
      841 rhfqlltvty gtgpapylal rvlqklakde gakyplalav frdqiyvddc afgadnpqla
      901 rqtrdhvisl lqrggftlrk wasncpelia didpsdhgha tskpllpden lkvlginwnp
      961 eadafrfnit intslpttkr eiistiakly dplgwaapiv ivakilmqql wlqkcewddp
     1021 iptelqhtwk tyytelpllr fmnlprwiah etripkielh gfadasqhay aavvysrvpt
     1081 nngdvvvsll aakskvaplk sltiprlelc galllarlma firtsfgniq celhgwtdst
     1141 itlawvhqpa srwktfianr vseihelmpk cqwhhvqsed npadcasrgm svpllinhsl
     1201 wwsgppwlks qsaswpgdis plettsllee rravtlhais spepwdlatr fsswtklirv
     1261 tagdslyskc vasnrnapst nflvkiypan gpsgggavla e
//
LOCUS       XP_076549169             125 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306508 [Osmia lignaria lignaria].
ACCESSION   XP_076549169
VERSION     XP_076549169.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693054.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478166) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="uncharacterized protein LOC143306508"
                     /calculated_mol_wt=14122
     CDS             1..125
                     /gene="LOC143306508"
                     /coded_by="XM_076693054.1:211..588"
                     /db_xref="GeneID:143306508"
ORIGIN      
        1 mrfsrkresc rndgmsrlsa revsqarlll rgragekspv vrerqktqip vpllqilpgs
       61 lvssigdwri lddlrrfpgq grlqvqqfly iwkignavne apekisetlp lqgvsredqg
      121 vsenr
//
LOCUS       XP_076549170             243 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306510 [Osmia lignaria lignaria].
ACCESSION   XP_076549170
VERSION     XP_076549170.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693055.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478166) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="uncharacterized protein LOC143306510"
                     /calculated_mol_wt=27102
     Region          137..230
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..243
                     /gene="LOC143306510"
                     /coded_by="XM_076693055.1:1..732"
                     /db_xref="GeneID:143306510"
ORIGIN      
        1 mlsdngtvfh gadrelrnmf rgasafylna aaslaaegtd wsfippyaph fgglweagvr
       61 tvkhhlrriv enatltfeem ttllcqveac vnsrplhpls ddpsdlsalt pghfligept
      121 civpdpesds hdislisrwr qisalrshfw hrwrreylqh lqqlpkwrqq kenlklgtlv
      181 llrdelqppa kwpmgrveal hpgpdgrvrv atlkttsgsv trpivklcpl pvpsadvtsp
      241 tst
//
LOCUS       XP_034194882            1305 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611094 [Osmia lignaria lignaria].
ACCESSION   XP_034194882
VERSION     XP_034194882.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338991.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194882.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1305
                     /product="uncharacterized protein LOC117611094"
                     /calculated_mol_wt=146825
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          <929..969
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1199..1291
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1305
                     /gene="LOC117611094"
                     /coded_by="XM_034338991.2:1..3918"
                     /db_xref="GeneID:117611094"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qarayssfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifatditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrrt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyedhshk atlhggtqlt lahirqtywi iggrapvksh
      961 ilrcvvcarq rgirahqlmg qlplsrvtps hpfshtgvdy agpialktwk grgaktqkgw
     1021 icvfvcmtts avhlemvtdy stdgfvatyr rfaarrgiph tiysdcgtnf igadsalksm
     1081 fvegtqdnqr laklfvddht twsfnppaap hmggkweavv ksvkyhlrrt vsdtlltfee
     1141 yytllnqiea tlnsrplepl tddpddlsvl tpahfligra istlpepsve qanisprarw
     1201 hliqqklqqf wkrwstqhlq rmqsiskwhh psnnihvgsl vlltderfpp ckwplarvla
     1261 vhpgpdgltr vvtiktatst ltrpiaklal lpvedqdssp sstsc
//
LOCUS       XP_034195329            1735 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611493 [Osmia lignaria lignaria].
ACCESSION   XP_034195329
VERSION     XP_034195329.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339438.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195329.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1735
                     /product="uncharacterized protein LOC117611493"
                     /calculated_mol_wt=193781
     Region          127..274
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          461..624
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          749..960
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(813..818,853..854,889,891..892,956..957)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(813..818,853,891)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            854
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          977..1137
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1358..1408
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1634..1727
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1735
                     /gene="LOC117611493"
                     /coded_by="XM_034339438.2:1..5208"
                     /db_xref="GeneID:117611493"
ORIGIN      
        1 mttsfsdllr eqeevagwiq rfwanvcklg kdkltgavle qrqgllnryw dtflsghrkl
       61 mrckeassss yvkedafsvt eeayldaasm ishhlkelps pcsshvqqsn iappphpqlp
      121 kidlpkfsgd plqwesfrdl fkslvhdvsh lpdvqkllyl kssltgeaae virntpitds
      181 gfrgawddle arygnirlls fshhralllc ppalqqsage lkrlldtfrq airayvtlrk
      241 pvtswdewfv yllsqkldkt thlawetsla dsreiprfqq lsqflenriq algaagmtdp
      301 slhavsptss keikpktkgg asakgvnsnv laaasksppa rkcpgcsgth glgfcykfka
      361 lspakrkerv qqlkaclscl nvghevgkcp skqvclacsk rhhtllheal tappasapgr
      421 eggqpareda astsddatqq vttlslsvgs kavallatak vrleapsget vevralldtg
      481 sdtslvsswv aqalrlprra vrvaisgvkd nevghatgev slvvrprhps dfrlpvralv
      541 lrkltsllps krivakswph itgltladpe ygvparvdll lgadvcgtlf gddsrrgpeg
      601 tptakltpfg wvlmgpasdd kpkaetaarv lhchgedsts qllqrfweld eirerapmsq
      661 eeekcerhfl dthardpsgr yvvrlpfvkd prtalksnrt talkllfnce rrlssdatlk
      721 eqyrnfmmey ltlqhmqgap eeadkgpayy lphhavrkrh dpsaklrvvf nasfctatgq
      781 slndclatgq klqadlwmvl trwrlfrvvf ttdivkmfrq irvhsedtkw qsilwratpd
      841 ervqdfrltt vtygtacapf lalrvlaqla ndessrfprg asvvrrhtyv ddiltgaddv
      901 setlalkrev vaifkaggfe lskwasnipe iqeadssntr lfqewsgist lgvnwnprdd
      961 afslrvaaae lvrecttkrs ilseiaglfd plgwaapvlv vakilmqdlw ilgadwdqql
     1021 penvctmwqr frgslpqldt lkiprwtfas sepstqmelh gfcdasqray aaavylrvnn
     1081 ngsvatsllv aktkvapvkt itipklelcg atllskllir vkeginmsgp tiawtdssvt
     1141 lhwirghasq wkpfvahrvs diqheipadn wrhirspdnp adlatrgmsp aelvdsdiww
     1201 hgpkwlteqp efwpatfpes negieaerrg atvhvvkekf genflcrfss lshllsviaq
     1261 cfrfaslsrg qpcekgfvka aerthaflav lrfsqhtsfl eeieqlqrhg elrkasplss
     1321 crpflddqgl lrlggrlena alpynekhpy lvakrcplar llirdahyrt lhggpqltrs
     1381 hlmrrvwivr grslvrseia acvtcarfsg rragqlmgql pqertaaqra flttgvdyag
     1441 piklrtspgr ghkaykgyia lfvclstrai hleavsdlts asflaafrrf vgrrghcatl
     1501 fsdngtvfqg adrelramfr qstlfyqeaa aslaldgtsw qfippyaphf gglweagvks
     1561 vkhhlrrvig dstltfeelt tllcqieacl nsrplyalse dpselaaltp ghfligeptt
     1621 vppepeepct alhtrwrlis qmraqfwkrw rreylqhlqq fpkwrryren leigslalir
     1681 ddlqppakwl mgrvtelhpg pdgcvrvvtl rtekgtvkrp ivklcplpvs ptdas
//
LOCUS       XP_076549171            1735 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306589 [Osmia lignaria lignaria].
ACCESSION   XP_076549171
VERSION     XP_076549171.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693056.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1735
                     /product="uncharacterized protein LOC143306589"
                     /calculated_mol_wt=193781
     Region          127..274
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          461..624
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          749..960
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(813..818,853..854,889,891..892,956..957)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(813..818,853,891)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            854
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          977..1137
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1358..1408
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1634..1727
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1735
                     /gene="LOC143306589"
                     /coded_by="XM_076693056.1:1..5208"
                     /db_xref="GeneID:143306589"
ORIGIN      
        1 mttsfsdllr eqeevagwiq rfwanvcklg kdkltgavle qrqgllnryw dtflsghrkl
       61 mrckeassss yvkedafsvt eeayldaasm ishhlkelps pcsshvqqsn iappphpqlp
      121 kidlpkfsgd plqwesfrdl fkslvhdvsh lpdvqkllyl kssltgeaae virntpitds
      181 gfrgawddle arygnirlls fshhralllc ppalqqsage lkrlldtfrq airayvtlrk
      241 pvtswdewfv yllsqkldkt thlawetsla dsreiprfqq lsqflenriq algaagmtdp
      301 slhavsptss keikpktkgg asakgvnsnv laaasksppa rkcpgcsgth glgfcykfka
      361 lspakrkerv qqlkaclscl nvghevgkcp skqvclacsk rhhtllheal tappasapgr
      421 eggqpareda astsddatqq vttlslsvgs kavallatak vrleapsget vevralldtg
      481 sdtslvsswv aqalrlprra vrvaisgvkd nevghatgev slvvrprhps dfrlpvralv
      541 lrkltsllps krivakswph itgltladpe ygvparvdll lgadvcgtlf gddsrrgpeg
      601 tptakltpfg wvlmgpasdd kpkaetaarv lhchgedsts qllqrfweld eirerapmsq
      661 eeekcerhfl dthardpsgr yvvrlpfvkd prtalksnrt talkllfnce rrlssdatlk
      721 eqyrnfmmey ltlqhmqgap eeadkgpayy lphhavrkrh dpsaklrvvf nasfctatgq
      781 slndclatgq klqadlwmvl trwrlfrvvf ttdivkmfrq irvhsedtkw qsilwratpd
      841 ervqdfrltt vtygtacapf lalrvlaqla ndessrfprg asvvrrhtyv ddiltgaddv
      901 setlalkrev vaifkaggfe lskwasnipe iqeadssntr lfqewsgist lgvnwnprdd
      961 afslrvaaae lvrecttkrs ilseiaglfd plgwaapvlv vakilmqdlw ilgadwdqql
     1021 penvctmwqr frgslpqldt lkiprwtfas sepstqmelh gfcdasqray aaavylrvnn
     1081 ngsvatsllv aktkvapvkt itipklelcg atllskllir vkeginmsgp tiawtdssvt
     1141 lhwirghasq wkpfvahrvs diqheipadn wrhirspdnp adlatrgmsp aelvdsdiww
     1201 hgpkwlteqp efwpatfpes negieaerrg atvhvvkekf genflcrfss lshllsviaq
     1261 cfrfaslsrg qpcekgfvka aerthaflav lrfsqhtsfl eeieqlqrhg elrkasplss
     1321 crpflddqgl lrlggrlena alpynekhpy lvakrcplar llirdahyrt lhggpqltrs
     1381 hlmrrvwivr grslvrseia acvtcarfsg rragqlmgql pqertaaqra flttgvdyag
     1441 piklrtspgr ghkaykgyia lfvclstrai hleavsdlts asflaafrrf vgrrghcatl
     1501 fsdngtvfqg adrelramfr qstlfyqeaa aslaldgtsw qfippyaphf gglweagvks
     1561 vkhhlrrvig dstltfeelt tllcqieacl nsrplyalse dpselaaltp ghfligeptt
     1621 vppepeepct alhtrwrlis qmraqfwkrw rreylqhlqq fpkwrryren leigslalir
     1681 ddlqppakwl mgrvtelhpg pdgcvrvvtl rtekgtvkrp ivklcplpvs ptdas
//
LOCUS       XP_076549172            1476 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306590 [Osmia lignaria lignaria].
ACCESSION   XP_076549172
VERSION     XP_076549172.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693057.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1476
                     /product="uncharacterized protein LOC143306590"
                     /calculated_mol_wt=164477
     Region          127..274
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          461..624
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          749..960
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(813..818,853..854,889,891..892,956..957)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(813..818,853,891)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            854
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          977..1137
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1358..1408
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..1476
                     /gene="LOC143306590"
                     /coded_by="XM_076693057.1:1..4431"
                     /db_xref="GeneID:143306590"
ORIGIN      
        1 mttsfsdllr eqeevagwiq rfwanvcklg kdkltgavle qrqgllnryw dtflsghrkl
       61 mrckeassss yvkedafsvt eeayldaasm ishhlkelps pcsshvqqsn iappphpqlp
      121 kidlpkfsgd plqwesfrdl fkslvhdvsh lpdvqkllyl kssltgeaae virntpitds
      181 gfrgawddle arygnirlls fshhralllc ppalqqsage lkrlldtfrq airayvtlrk
      241 pvtswdewfv yllsqkldkt thlawetsla dsreiprfqq lsqflenriq algaagmtdp
      301 slhavsptss keikpktkgg asakgvnsnv laaasksppa rkcpgcsgth glgfcykfka
      361 lspakrkerv qqlkaclscl nvghevgkcp skqvclacsk rhhtllheal tappasapgr
      421 eggqpareda astsddatqq vttlslsvgs kavallatak vrleapsget vevralldtg
      481 sdtslvsswv aqalrlprra vrvaisgvkd nevghatgev slvvrprhps dfrlpvralv
      541 lrkltsllps krivakswph itgltladpe ygvparvdll lgadvcgtlf gddsrrgpeg
      601 tptakltpfg wvlmgpasdd kpkaetaarv lhchgedsts qllqrfweld eirerapmsq
      661 eeekcerhfl dthardpsgr yvvrlpfvkd prtalksnrt talkllfnce rrlssdatlk
      721 eqyrnfmmey ltlqhmqgap eeadkgpayy lphhavrkrh dpsaklrvvf nasfctatgq
      781 slndclatgq klqadlwmvl trwrlfrvvf ttdivkmfrq irvhsedtkw qsilwratpd
      841 ervqdfrltt vtygtacapf lalrvlaqla ndessrfprg asvvrrhtyv ddiltgaddv
      901 setlalkrev vaifkaggfe lskwasnipe iqeadssntr lfqewsgist lgvnwnprdd
      961 afslrvaaae lvrecttkrs ilseiaglfd plgwaapvlv vakilmqdlw ilgadwdqql
     1021 penvctmwqr frgslpqldt lkiprwtfas sepstqmelh gfcdasqray aaavylrvnn
     1081 ngsvatsllv aktkvapvkt itipklelcg atllskllir vkeginmsgp tiawtdssvt
     1141 lhwirghasq wkpfvahrvs diqheipadn wrhirspdnp adlatrgmsp aelvdsdiww
     1201 hgpkwlteqp efwpatfpes negieaerrg atvhvvkekf genflcrfss lshllsviaq
     1261 cfrfaslsrg qpcekgfvka aerthaflav lrfsqhtsfl eeieqlqrhg elrkasplss
     1321 crpflddqgl lrlggrlena alpynekhpy lvakrcplar llirdahyrt lhggpqltrs
     1381 hlmrrvwivr grslvrseia acvtcarfsg rragqlmgql pqertaaqra flttgvdyag
     1441 piklrtspgr ghkaykgyia lfvclstrar tlrhsv
//
LOCUS       XP_034194538             470 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610817 [Osmia lignaria lignaria].
ACCESSION   XP_034194538
VERSION     XP_034194538.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338647.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194538.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="uncharacterized protein LOC117610817"
                     /calculated_mol_wt=51953
     Region          141..285
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..470
                     /gene="LOC117610817"
                     /coded_by="XM_034338647.2:1..1413"
                     /db_xref="GeneID:117610817"
ORIGIN      
        1 mpkaldqsts qstsqginls tlrrkrgniv ghitafskll dtakkseqrd lgllrahltn
       61 lyeiwdrfda iqleleeade sevprryqih gdyiavvasa naliegdrpr mpprrittds
      121 sapsvtapma iklpemrlpt fdgkienwas yfeslssmid qnadlslvqk lqylrstltg
      181 kaaaciqsls ttdsnyadai ellkekfdck rrillkhcna iiglpkitrd spealgdlvd
      241 tvrqnlrslk nlevdtsswd siliaiiltk lstetvyqwe lslkdkqmpt fasllefldk
      301 ranciimthq kpaqtdgqhn rgppgksivk qssrshafvt ttranetqns retvkpsgts
      361 anyprcpicq dehgiwrcek fhalsvgarl savrkaslcs nclraehnle lckkgscric
      421 kkhhhtllhl ptqvvnrptt tpptklstsa gtsagasagt padtqvdtge
//
LOCUS       XP_076549173            1209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306591 [Osmia lignaria lignaria].
ACCESSION   XP_076549173
VERSION     XP_076549173.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693058.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1209
                     /product="uncharacterized protein LOC143306591"
                     /calculated_mol_wt=138454
     Region          <2..77
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          207..421
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(271..276,310..311,346,348..349,412..413)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(271..276,310,348)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            311
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          437..597
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          814..864
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          909..1003
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1093..1184
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1209
                     /gene="LOC143306591"
                     /coded_by="XM_076693058.1:1..3630"
                     /db_xref="GeneID:143306591"
ORIGIN      
        1 mipkiaslip dqqidrsnik ipknlnladp efhrpapidl llgsgtalsl vsvgqinlsp
       61 pdgpdlylqk trlgwviggs ppkpiksriv scnattalqh dlarfweiee gpqlqllses
      121 dkfcekhfqq hvkrnsdgry vvalpfndkl lllgesrara lkrleslerk lqrdatlrge
      181 yhaviqeyld lghmtkvtni qcpsdgyylp hhgvtkitse ttklrvvfdg saatssgisl
      241 ndalhigpri qedllyillr frvhryvitg diekmyrqfl vrdedqkyqr vlwrdasrei
      301 qtyelrtvtf glsaapylai rcltqlaqde chrfpraaqi lrrdfyvddl ltgastlqea
      361 aslredltql lklaslnirq wasnhedllr nlpeesinrk irigesstlk tlgiiwdsan
      421 dsisyevktq iansyvtkrf itseiakiyd plgligpvii vakmllqriw tikvdwdesl
      481 pmdihtewkq fhrqlpllns tvfrrrtvid tpsrielhgf cdasekayga ciyirssdmh
      541 dnttvellla kskvaplktq siprlelcga lllttlyntv kkalhvklnr tifwtdstvv
      601 lhwlqtspht lktfvanris eiqartnigd wrhvpttdnp adlisrgqhp eeflqpsiwh
      661 hgpewlkhee vtwptrdlvp cnfipeqrta tclkvspldt nilddysswe kmqrivawcl
      721 rwkgdnmikg pltpvelrha rdviikllqr ihfskelrcl ssssnpeiag klqrlspfvd
      781 kdgivrvggr lkhssmpftq rhpiilpkar vtgliieaeh raqlhagvqg tlyavrrrfw
      841 pidgrnqvwk alrtctrclr arppatnyim gelpearvte arpftnvgvd ycgpfyiker
      901 rhrnrtrikv yvavfvclav kavhlelvsd ltseafiaal rrfiarrgyc vkihsdngtn
      961 fvganneiqe iqrlirsdeh ntkvqnflaq rsiewsfipp raphfgglwe aavksfknhl
     1021 lrvagtelft fedfntliie iesilnsrpl tpmssdpndl lvltpghfli gdsltslrer
     1081 dyldtppnrl sswqfiqrik qhfwtrwhre ylneltrrtk wskgehpike gtlvllredn
     1141 vppmqwalgr vlrtypgsdg iirtvtikta aseldrsvkr lvplpyqvee rsnepeepas
     1201 tanvnpidd
//
LOCUS       XP_076549174             241 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306592 [Osmia lignaria lignaria].
ACCESSION   XP_076549174
VERSION     XP_076549174.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693059.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="uncharacterized protein LOC143306592"
                     /calculated_mol_wt=26793
     Region          141..233
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..241
                     /gene="LOC143306592"
                     /coded_by="XM_076693059.1:1..726"
                     /db_xref="GeneID:143306592"
ORIGIN      
        1 mcsiltsdqg tnfvgadrel rrlfsaakfd fntlasslal dgttwkfnpp aaphfggiwe
       61 aavksmkfhl rrvvgetplt feemstvlaq ieaclnsrpl aplgtdpdcn efltpghflv
      121 gapltavpep slidqkparl trwqllqqmr dhfwkrwaae ylqhlqartk wsqpqpnaqp
      181 gdlcmvtsea tpptkwpigr iietypgddn kvrvvlvrtp fatlkrpvtk lclfpttpag
      241 n
//
LOCUS       XP_076549175             288 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306594 [Osmia lignaria lignaria].
ACCESSION   XP_076549175
VERSION     XP_076549175.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693060.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="uncharacterized protein LOC143306594"
                     /calculated_mol_wt=31970
     Region          2..184
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(16,70,106,108,155,180..181)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(16,180)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(70,108,181)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..288
                     /gene="LOC143306594"
                     /coded_by="XM_076693060.1:1..867"
                     /db_xref="GeneID:143306594"
ORIGIN      
        1 mvsaaelrad lvvvsepyti pdnwfgdttg raaifitsag lakckktsii gsgngyvgis
       61 yngvgivsvy ispnashgev esqfaaldyf vkelsrshsr ilvmgdfnak splwgtsswc
      121 rrgrelfqlg tglglrpiia eggvtcergr gsmidillgs dmalkahkss vvreefsasd
      181 hkyilhsfsv gvdtgteiqd pfnlkgkide eqvlvallkk ygdenfpkla ktrtswevdr
      241 fiedvgiiin rhtvyrrpfq gnhkpaywwt eataekrrev nrmrrkyt
//
LOCUS       XP_076549176             974 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306595 [Osmia lignaria lignaria].
ACCESSION   XP_076549176
VERSION     XP_076549176.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693061.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..974
                     /product="uncharacterized protein LOC143306595"
                     /calculated_mol_wt=106347
     Region          <153..232
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     Region          275..427
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          561..772
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(625..630,665..666,701,703..704,763..764)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(625..630,665,703)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            666
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          789..>823
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..974
                     /gene="LOC143306595"
                     /coded_by="XM_076693061.1:1..2925"
                     /db_xref="GeneID:143306595"
ORIGIN      
        1 mggfgaalrk stpavlrsss rpanlsvspe tvdggtqasp ghlpsgnpgl wslaeagsll
       61 grmvrlhpdt eagqvthlaw etslanskdi ppfhqlaafl enqiqamdaa qlgdpllhpa
      121 gpksakeakp kvkeeavkkr ssttvltaaa kstqprkcps csgnhslgyc ykfkalapsk
      181 rkeraqtlka csncltvghd sskcpskqsc lacsgrhhtl lydalkitps tskqdtmeav
      241 ptgsssslsd epssatslsl tagprsaall atakvkleap sgetlevral ldtgsdaslv
      301 sswvvqalrl prravrvais gvqenqngvs tgevslvvrp rqpsgfrlpv ralvlrklts
      361 llpahriapq pwphlkglpl adpeygvpar vdlilgadvc galfadgtrt gfpgtptakl
      421 tpfgwvlmga ilcapaqeea rtfhcrtean vgqllqrfwe leevhnhptm seeeercarh
      481 fkgthardak gryvvrlpfv repaaslkps rtsalkllfn cerrlasdna lkdkcgkfle
      541 eylslqhmkt vpeaakeepa yylphhavvk rhdptaklrv vfnasfrtas gyslndclsa
      601 gpklqadlwm vltrwrifkv vfttdvvkmf rqiqvhpedt kwqrilwrtg pdervqdfql
      661 itvtygtaca pflalrvlnq laedegdrlp lgagairrht yvddilagad nvnealrvkh
      721 qviqifkagg fdlskwasnv pklrengasd qhlfqewpgi atlgvnwdpt tdsfslrvap
      781 padsppastk rsilsevasl fdplgwvapv lvtakilmqa wtdssvclyw irghasqwkp
      841 fvahrvsaiq selppdlwrh vassdnpadl atrgispadl lkaelwwhgp swlprsasqw
      901 paerleksga mdlaqskltv hlavedksde lltmysslsr lltvlaycfr fsnlarkrpc
      961 esgfikaeer asal
//
LOCUS       XP_076549177             697 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306596 [Osmia lignaria lignaria].
ACCESSION   XP_076549177
VERSION     XP_076549177.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693062.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="uncharacterized protein LOC143306596"
                     /calculated_mol_wt=78520
     Region          <68..155
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          281..441
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          566..>687
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(631..636,671)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            672
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     CDS             1..697
                     /gene="LOC143306596"
                     /coded_by="XM_076693062.1:1..2094"
                     /db_xref="GeneID:143306596"
ORIGIN      
        1 mkkmgtakit kgvitsrlel lerhwttfse thneildayl ktgsespyfk qdymgtceea
       61 flqrfcgawa vlteryqnlr vlinfhlttl mshspmkkes arelkslmnr ttdavqalea
      121 lkrpvqywdd wlvfitiekl dqktfmawet svgstaespt ftqlhtflts rsraleaveg
      181 apgpsttptg tsntlkqkkk qpinsnlrfk ahathtageg nrrcaicnke hfllfcstyk
      241 gkhhtslhha seqptairns fppqvpesnt ttpeslpler vsevtgrsth camtscsais
      301 svsvvlatae lmvevpekip vigvgktqtc tkgrvsvrim srlnksisyn lnahvlprls
      361 syqtakscsa siaylegltl adpnflssrr inlilgadvy aqiitaglkh eppntpvaqa
      421 ttlrwiltgp lrqsensdtq tnsaislqcs vresnlietl trfwkleeip sasnplneee
      481 nicedhfskt hkrdnsgryv vrlpfksspe klgesrgaac tllaklerrf rlnvnlqtay
      541 rdflkeycdl ghmeripesi qtpgltyylp hhgvmresss ttklrvvfna sfktssgvsl
      601 ndlvhkgpnl lpeifslllr wrkhaivfsp dvekmyrqir vagedcdfqr ivwrdtpdes
      661 lktyrlttvt ygmacapylt irtlhqlsrn etfiwtt
//
LOCUS       XP_076549178             187 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306597 [Osmia lignaria lignaria].
ACCESSION   XP_076549178
VERSION     XP_076549178.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693063.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 33% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="uncharacterized protein LOC143306597"
                     /calculated_mol_wt=21088
     Region          71..>170
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..187
                     /gene="LOC143306597"
                     /coded_by="XM_076693063.1:1..564"
                     /db_xref="GeneID:143306597"
ORIGIN      
        1 mrqdnnyrka itaqerlvvt lrvlatddsf tslqylfkis eqngtetgte wlkiaqqynd
       61 twnfphcigs idgksiqspk hsgsdyinyk gffsvvllsv vdanynflfv dvgcqgrisd
      121 ggvfnnssly rameekklni paairlegre tpipffflgd ealpisehll kcysgyftkg
      181 sqqrmve
//
LOCUS       XP_076549179             350 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306598 [Osmia lignaria lignaria].
ACCESSION   XP_076549179
VERSION     XP_076549179.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693064.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="uncharacterized protein LOC143306598"
                     /calculated_mol_wt=38969
     Region          <13..>69
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Region          <101..197
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Region          <275..313
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..350
                     /gene="LOC143306598"
                     /coded_by="XM_076693064.1:1..1053"
                     /db_xref="GeneID:143306598"
ORIGIN      
        1 mkfkdevlas kplhpsqhay qngkstetal hklvgliega lsigesalci fldiegafdn
       61 tphdaiqeaa rrynikdsvv rwihnmltnr taiaslgeet lghhvtvfqa ellaiwacak
      121 ydlerahsgk hiyicndsla alralhkvei gsklvrdcal tlrqltlnnr vklvwvpgha
      181 gipgnerane larlgatssq pyheypigvs tyalkglakg wlnqeftriw hnangmrhtr
      241 allegpsqkl gdtlihldra qlrmlvglmt ghwytrkhlv rmglieestc prceeedetp
      301 lhvllrcrel ralrgsilgt lepsslsisa rlvptllkla tetqlprhsq
//
LOCUS       XP_076549180             316 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306599 [Osmia lignaria lignaria].
ACCESSION   XP_076549180
VERSION     XP_076549180.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693065.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="uncharacterized protein LOC143306599"
                     /calculated_mol_wt=35149
     Region          40..131
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          219..312
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..316
                     /gene="LOC143306599"
                     /coded_by="XM_076693065.1:1..951"
                     /db_xref="GeneID:143306599"
ORIGIN      
        1 msdlptarvt parpfahcgv dyagpfsala hrgrgqkahk vyialficls traihlelvr
       61 dystaaflaa fnrftarrgl ptklysdngt nfqganrelr aafklvtads hlralfaadh
      121 itwhfippss phfggiweag vksvkthlrr mlcdrtptce efntllcqie aclnsrpltp
      181 lkddlescea ltpghflvgg vlkvppapsv idiaenrlsr wqwvqqltek fwkawaheyl
      241 qslqarskwc sratnlkigd lvllksstlp pgkwdlgrvt kchpgkdglv rvvsiktars
      301 eytrpvvnlc klpiss
//
LOCUS       XP_076549182             324 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent DNA helicase Pif1-like [Osmia lignaria lignaria].
ACCESSION   XP_076549182
VERSION     XP_076549182.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693067.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..324
                     /product="ATP-dependent DNA helicase Pif1-like"
                     /calculated_mol_wt=35889
     Region          37..322
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(68..74,146)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..324
                     /gene="LOC117611166"
                     /coded_by="XM_076693067.1:1..975"
                     /db_xref="GeneID:117611166"
ORIGIN      
        1 mvdwtnlnfp eppvsafgql rpfdkyvipk genvgrlnag qlsaynaiia tlspsyagpt
       61 yffldgpggt gktflykali qyfrnnghvv vpsawtgiaa lflpgghtsh fffkfpvplm
      121 edsicglcra svgdrrlida kliimdeayi cerqalmamd rilrdltevr hrpfggnill
      181 lggdfrqcap vipnapagsn iaesirssnl wsefkllhlt qnmrvgpgeq yfagylltig
      241 ngsvnvpddi lfhgspqdli hwvyennlaq pnpnrallcp rndhcdyiyn lildllegdq
      301 rvyfsedkli dppaeaiady ppis
//
LOCUS       XP_034194556             317 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein C21orf140 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034194556
VERSION     XP_034194556.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338665.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194556.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="uncharacterized protein C21orf140 homolog"
                     /calculated_mol_wt=36154
     Region          <1..232
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     Region          <93..256
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..317
                     /gene="LOC117610835"
                     /coded_by="XM_034338665.2:1..954"
                     /db_xref="GeneID:117610835"
ORIGIN      
        1 mgfrwkkstn vrkilvekpn iiawrdkylr aidsyrrqgr niiyldetwv dntlsfskcw
       61 qsneeigvlk nnssshgyii vhaggeggfv kgaalifkar sttgdyhgqm nalnfkkwvs
      121 qkllpnipsq svivmdnapy htvqinkppt aygkksdmik wleenniqht atmkkyqlve
      181 liqrnksldk tyktdelfkr hghnvlrlpp ymcelnpiel vwakvkriir enntstltai
      241 eltnltekav ndvstedwvg fckhvqkiee qyverdname nimeeivdar pmnasscsds
      301 sdsdtnssss ssssvqd
//
LOCUS       XP_076549183             488 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306600 [Osmia lignaria lignaria].
ACCESSION   XP_076549183
VERSION     XP_076549183.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693068.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478167) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..488
                     /product="uncharacterized protein LOC143306600"
                     /calculated_mol_wt=54682
     Region          12..205
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(36,90,126,128,175,200..201)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(36,200)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..488
                     /gene="LOC143306600"
                     /coded_by="XM_076693068.1:1..1467"
                     /db_xref="GeneID:143306600"
ORIGIN      
        1 maypktrpdt wrhrlahdll avsareqdad ilvlaepylt penwftdltg rasisvtnsg
       61 igksrkltvl ncnkgfvcin yndicimsvy aspndsnqef eaqlaamemf lasirgalak
      121 vivlgdfnak splwgskkwc srgrtlyqwc nglglvptia kggatcergg gsvvdllfcs
      181 ndayklhvts evldiytasd hkyivrkfrg ttpnasvtnd lfdlgkgkid eaglldelir
      241 kygddaydrr vktgttlevd kfvedverlv nkhtsyrgpy kgnktqvyww neetakcrke
      301 vnkarrrytr lrakgnandi egalkefkma gkkmkrtvdk akrtswkdml gdvdkdiwgr
      361 pytlvtkklk gknskpdils sdeveavvkk lfilsppsmg tpekdieqei rdnareiret
      421 tervnanaev rdedegpvtv rqrrmggitp eevisvvrcl pvnkapgpdr mptivakkfs
      481 lgaarwla
//
LOCUS       XP_034194578             803 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610860 [Osmia lignaria lignaria].
ACCESSION   XP_034194578
VERSION     XP_034194578.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194578.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..803
                     /product="uncharacterized protein LOC117610860"
                     /calculated_mol_wt=91294
     Region          665..>769
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(721,755,765,767)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..803
                     /gene="LOC117610860"
                     /coded_by="XM_034338687.2:142..2553"
                     /db_xref="GeneID:117610860"
ORIGIN      
        1 msdsenvfti eviedrrnec eqqcvmants rrpkltvhtr ryskkracre rrrrlalqnt
       61 anckeifeet tnelfeieet nnaedginme gtnseneelm iqkvvtnepe eqfclpsgls
      121 ivdmqhifae fqkigdhakn redcgirhlk itgmkrsglr ttlsvicdmc kytgeihsep
      181 desnhmetnq cavagtivng asyaqmeefl aamnipcmsk kefrkhhdei vktlivaaee
      241 emvtaaeeek rlaiergdiv sgcgiphipv vadgswmkrs yrtgtynsas gvgviigyht
      301 kkvlfigvrn kvcricrcaa kkkeeprkht cfknwsasqa stamesdaiv egfktsvekr
      361 gliystliad gdssvykkii dadpykaqir vkkiectnhl lrnfcrkmke vvkmttpgpf
      421 rrivessirr mradivkaae yrysqqvtta ekiknlyadi hnvpshvfge hadcsklqyf
      481 cdgtskkdek nivpelmkmg vyeqikdilr pllahaesll ynsnnnaves fngiiakytg
      541 gkrlnfgerg sytgrcaaav lqyntkqvls rlntamnkep pvilrkienr rkkenekyse
      601 krkekkitkh vrkqfrsekd snygpnaekp dmanniyele qkkhmemlqn wqnrrdaiee
      661 etigqaknpr wlnykskllm asnfgricrr rigtlcgnsv kslvyprlis apavqygqec
      721 ekfareqlsv cigveikecg lfidsaipfl gaspdghide egivkikcpk taenllpeea
      781 iksvavirri fadakgneln rnh
//
LOCUS       XP_076549185             424 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306623 [Osmia lignaria lignaria].
ACCESSION   XP_076549185
VERSION     XP_076549185.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693070.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..424
                     /product="uncharacterized protein LOC143306623"
                     /calculated_mol_wt=46901
     Region          <300..>394
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..424
                     /gene="LOC143306623"
                     /coded_by="XM_076693070.1:3217..4491"
                     /db_xref="GeneID:143306623"
ORIGIN      
        1 mppfklkymr akrsrkiglr gntflveeln idssnvesss ssgasstdlq skelglvpir
       61 qlsqgidtvm anstsipfns titdvplsst tvaaavsknt ptsiesssil sesylrlslv
      121 kdlipqfdgk sssltefike cqlisskvkp edragllfli rgkivghakl ilsnrrepnt
      181 ldeligllkk ayvrsfnmhs ineefmglkq gtndsvevfg srvskilstg ievakdtyns
      241 eqlsgvvdll nntaiatfin glrngmlrim lkdkdskeip dleavidiar rlesvtvegs
      301 rlspssqpli gnanvrkidf ddrrcfqcnk vghlrshcpq iqetrrnplr qeqircryck
      361 ktghmearcf rkqgqnspqp qgvfphkitd qghlnskgvp rrgvarnepw iapfkpvgpa
      421 prsk
//
LOCUS       XP_076549187             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306625 [Osmia lignaria lignaria].
ACCESSION   XP_076549187
VERSION     XP_076549187.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693072.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC143306625"
                     /calculated_mol_wt=16930
     CDS             1..150
                     /gene="LOC143306625"
                     /coded_by="XM_076693072.1:503..955"
                     /db_xref="GeneID:143306625"
ORIGIN      
        1 mcyireqpte rdtsriellm nltarlcmrs tcyrldvgiv cmqcaleaal gaariydmve
       61 vhyplptayp pgacdicsap lmesqaayhc eeciaaiime meyqranqpi pppaivptwy
      121 terqirsrqp gprrtfrlht avssitnhht
//
LOCUS       XP_076549188             232 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306626 [Osmia lignaria lignaria].
ACCESSION   XP_076549188
VERSION     XP_076549188.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693073.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..232
                     /product="uncharacterized protein LOC143306626"
                     /calculated_mol_wt=26677
     CDS             1..232
                     /gene="LOC143306626"
                     /coded_by="XM_076693073.1:274..972"
                     /db_xref="GeneID:143306626"
ORIGIN      
        1 mysqslkgff crrkhpssie eetrkkitkh qddvkikpqr scqifgrkyp ltdsctkhle
       61 iginifgnkf ypqlilsdsr nnqlelsivt wslivaeadn vrsffedatd trdninfndv
      121 cvefgvlyge kvlklfnkdk cmcmirktfe kmvilndcii emhnalydtl ysveqkfkef
      181 vtlvrgygdv kehaevvlyi keshiydrnl iidgelvalg ihaiitaast as
//
LOCUS       XP_076549190             770 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611170 [Osmia lignaria lignaria].
ACCESSION   XP_076549190
VERSION     XP_076549190.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693075.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..770
                     /product="uncharacterized protein LOC117611170"
                     /calculated_mol_wt=87819
     Region          432..581
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          <683..>756
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..770
                     /gene="LOC117611170"
                     /coded_by="XM_076693075.1:3508..5820"
                     /db_xref="GeneID:117611170"
ORIGIN      
        1 mnnknlkptd nknttdgnrl trsrtqenpe lksivedrny asaflqsrkt prspterrtf
       61 dftpevtaea snantnlgai prtknvknlt vntnqtengs anwqnksigf tinpsneadn
      121 nkqtgtfasp lirispqdnr nnteilneti nnsckndisl leiegiteqp slignldgen
      181 segnltltcv sprkkmtdqq iqiqplrlpl kyvanlvpef dgknisvtey vekvkhakni
      241 itpvdepsli pilkiklkge vykalinspi nnvndfiqai kslfpsteni hslygkltel
      301 tqkpgetvls ygnrlqelvi qikdlkrmet ginqqiiqdf drtikndavk sfknglkqei
      361 rielkqkddl nvliqeaidi esrlkkqnml rggdsasvlc slekydanpv tytcqickel
      421 nhealfcnna acvycknkdh isynckfarn kielickycn tqghsidacr mnrskgnycq
      481 ycqvmghtvt qcpfiieyet cwkckgsghd pntctrfpki telceiynsr yhtaencptp
      541 mcplcnklgh tikhcpltkn nrlqlimctn cneeghdaee cegarvfqtr thpikyqnrn
      601 qlghsagsyy elrnpqrrsn dlygdnhnfn hnnsnfqrky nyngnfngnn hfrnnngngn
      661 fnsnnysrnn nsnrdnprht neprkfcdyc rtpnhtikec rklkaielra qkrdicsyce
      721 epghlidacr klkslensag kicnlckstn htteechrgq nhqqhrqgne
//
LOCUS       XP_076549191             770 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611170 [Osmia lignaria lignaria].
ACCESSION   XP_076549191
VERSION     XP_076549191.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693076.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..770
                     /product="uncharacterized protein LOC117611170"
                     /calculated_mol_wt=87819
     Region          432..581
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          <683..>756
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..770
                     /gene="LOC117611170"
                     /coded_by="XM_076693076.1:1587..3899"
                     /db_xref="GeneID:117611170"
ORIGIN      
        1 mnnknlkptd nknttdgnrl trsrtqenpe lksivedrny asaflqsrkt prspterrtf
       61 dftpevtaea snantnlgai prtknvknlt vntnqtengs anwqnksigf tinpsneadn
      121 nkqtgtfasp lirispqdnr nnteilneti nnsckndisl leiegiteqp slignldgen
      181 segnltltcv sprkkmtdqq iqiqplrlpl kyvanlvpef dgknisvtey vekvkhakni
      241 itpvdepsli pilkiklkge vykalinspi nnvndfiqai kslfpsteni hslygkltel
      301 tqkpgetvls ygnrlqelvi qikdlkrmet ginqqiiqdf drtikndavk sfknglkqei
      361 rielkqkddl nvliqeaidi esrlkkqnml rggdsasvlc slekydanpv tytcqickel
      421 nhealfcnna acvycknkdh isynckfarn kielickycn tqghsidacr mnrskgnycq
      481 ycqvmghtvt qcpfiieyet cwkckgsghd pntctrfpki telceiynsr yhtaencptp
      541 mcplcnklgh tikhcpltkn nrlqlimctn cneeghdaee cegarvfqtr thpikyqnrn
      601 qlghsagsyy elrnpqrrsn dlygdnhnfn hnnsnfqrky nyngnfngnn hfrnnngngn
      661 fnsnnysrnn nsnrdnprht neprkfcdyc rtpnhtikec rklkaielra qkrdicsyce
      721 epghlidacr klkslensag kicnlckstn htteechrgq nhqqhrqgne
//
LOCUS       XP_034194997             371 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611171 [Osmia lignaria lignaria].
ACCESSION   XP_034194997
VERSION     XP_034194997.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339106.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..371
                     /product="uncharacterized protein LOC117611171"
                     /calculated_mol_wt=42305
     Region          60..209
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(62..65,96..98,101,126,187,205)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..371
                     /gene="LOC117611171"
                     /coded_by="XM_034339106.2:158..1273"
                     /db_xref="GeneID:117611171"
ORIGIN      
        1 mesaragaer pvvelaacpe pwikwragem mvlpeaalms vpaetrqryg sideefaemy
       61 tdgskregaq avgaavvtkq qnnweentfs mnpgatifta eavavaealr lysqsgdgrr
      121 llvysdsasv lkvleaaknk dikdivkmkr geivakimke lceiylmknl plnetvnrkk
      181 tpvvfvwvpa hrgiagnera daaakrateq tpnceyalpy edmlvnlvrn awkefatemr
      241 nearykdvky fseidtnkqr rkpwfekfvt qdrrtisvls riranhynls eslarkhvve
      301 dhgcdcrehv edlahvlwec ekydhirdef vakmrekgfs grpqyqdlvr kerpavcvav
      361 arfliktgri i
//
LOCUS       XP_034195148             241 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611312 [Osmia lignaria lignaria].
ACCESSION   XP_034195148
VERSION     XP_034195148.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339257.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195148.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="uncharacterized protein LOC117611312"
                     /calculated_mol_wt=26859
     Region          137..225
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..241
                     /gene="LOC117611312"
                     /coded_by="XM_034339257.2:1..726"
                     /db_xref="GeneID:117611312"
ORIGIN      
        1 mlsdngtvfh gadrelrnmf rgasafylna aaslaaegtd wsfippyaph fgglweagvr
       61 tvkhhlrriv enatltfeem ttllcqveac vnsrplhpls ddpsdlsalt pghfligept
      121 civpdpesds hdislisrwr qisalrshfw hrwrreylqh lqqlpkwrqq kenlklgtlv
      181 llrdelqppa kwpmgrveal hpgpdgrvrv atlkttsgsv trpivtplpv psadvtspts
      241 t
//
LOCUS       XP_034195149             792 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611314, partial [Osmia lignaria
            lignaria].
ACCESSION   XP_034195149
VERSION     XP_034195149.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339258.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195149.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..>792
                     /product="uncharacterized protein LOC117611314"
     Region          <1..106
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          291..443
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          580..791
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(644..649,684..685,720,722..723,782..783)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(644..649,684,722)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            685
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     CDS             1..792
                     /gene="LOC117611314"
                     /coded_by="XM_034339258.2:<1..>2376"
                     /db_xref="GeneID:117611314"
ORIGIN      
        1 aeviqntpit dagyqgawed leqrygnlrl lsfshhrall scppaqqqsa aelkrlldtf
       61 rqsiraytal nkpvpqwdew fvyflsqkld ktthlawets ladsrdipsf rqlslflenr
      121 iqaldaaqaa dpvlhpattk galaktnkea svkrskvavl aasakspapr kcpscagnhn
      181 faycsklkal pprrrkeraq qlqacfnclt vghdsnkcps sqvclacgnk hhtllhdama
      241 spaksaptak gdaptspppa vfsdieeknv aasfacsatt rpaallatak vrveapsgeh
      301 levralldtg sdtslvsgwv aqalrlprra vsvaisgvqe nesglaksev sfslrsrldp
      361 sfhlsvralv lrkltallpa ravqpqswph lqgviladpe ygvpsrvdlt lgadicgvll
      421 qdesrvgpmg tptarrtplg wvlmgpvsda kgpsskavvh slhcqtddst nrllrrfwel
      481 eelheqppls pedrecerhf rethyrdetg rysvhlpfkl dpslalkpsr stalklllsc
      541 erklasneaw rdkysqfmde yaalshmeav pesavqdpay ylphhavakr ydptgkirvv
      601 fnasfrtatg sslndcllsg pklqsdlwmi ltrwrlfrvv ftsdivkmfr qirvdpadad
      661 wtrilwrsrp dqpvqdfrlk tvtygtacap flalrvlvql aadeeerfpl gavairrhsy
      721 vddilagadd eeaalelrrq visilqsggf dlskwasnkp slqeaeesqa cvfqdhtgvs
      781 tlgvvwrptt dt
//
LOCUS       XP_034195041             607 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611214 [Osmia lignaria lignaria].
ACCESSION   XP_034195041
VERSION     XP_034195041.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339150.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195041.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 49% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..607
                     /product="uncharacterized protein LOC117611214"
                     /calculated_mol_wt=70088
     Region          70..168
                     /region_name="RT_RNaseH_2"
                     /note="RNase H-like domain found in reverse transcriptase;
                     pfam17919"
                     /db_xref="CDD:465567"
     Region          474..>539
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..607
                     /gene="LOC117611214"
                     /coded_by="XM_034339150.2:603..2426"
                     /db_xref="GeneID:117611214"
ORIGIN      
        1 mlsrthtkwe syktdpakte avqrfptpkn vknireflel agyyrrfihn fakiakplti
       61 llqkdnkffw nteqqeafik lkeilcnapi lqypkfdepy vittdaskya lgavpsqaei
      121 gkdrriayas rvlqeaeiky dtyekealai mfaiktfrny iygkkftivt dhkplfwlkt
      181 adnktrvqkw rlklsdyeyd iaykparaqg kkeisknstp akilnkdkve astdnhikvk
      241 gkrgrprkni qeplaqetts nsnnkgkttk rkeeedktek dniqeqeqkn liytknsiqf
      301 rndnivhiit qqggaqndes sidrkdhqnn kadlldhlnq pwlthpnhpk sgvrcktahk
      361 nhwrflekrg ildsfkdese hlskeeldda kaqfdeaikv fnkehahfev vwttsqanhp
      421 yftddchyqm qqigtmakki iakrsaelpa qttaqpgkvt csksklpdie lprftgnyte
      481 wssflelfns vvcgkpelkq vekfyclkgc lrgepaqivs slpligasls aaiellkqrc
      541 terwtarrrs dgsprlesql sthrlrssws lshpgsgrws tsqtirpevl lrslrrqspl
      601 nhelspq
//
LOCUS       XP_034195039             813 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611213 [Osmia lignaria lignaria].
ACCESSION   XP_034195039
VERSION     XP_034195039.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339148.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195039.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..813
                     /product="uncharacterized protein LOC117611213"
                     /calculated_mol_wt=92983
     Region          133..272
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          457..596
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          728..>807
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..813
                     /gene="LOC117611213"
                     /coded_by="XM_034339148.2:1..2442"
                     /db_xref="GeneID:117611213"
ORIGIN      
        1 mpntqestql kvlrkqgasi kaqitriesy lnegehfdvh dlqsrkerai elgrkfeeiq
       61 lsieiedees dhnverenfe rnyfrimsii nrriegfrap htlinsgrvt espntvmirq
      121 estllpkiei kpydgnpiew hsfhdtfktv vhdnqdiplv qkfhllknal rgeiasvisa
      181 lnasepnylv awellqrrcn kprqivqshl kilfeltevt rdtpanlrsl aeqaqmhvea
      241 latlgqpttq wdailvylig kkldkntrrg wertleidem ptfeqllkfi nkqargeele
      301 tevpttsrgn agqdrfqrsr vsskghtyva manvnkcvmc kdnheiyqck qflqaavrdr
      361 ldvikrsrlc lncfrsnhlv nnchangckk cnqkhntllh snnenrvnts nnttqvgnqa
      421 snsgtnatal tvtcgsevll gtarvkildk fnnehdcrvl ldggsqthfi tnelaeklql
      481 ekhsvsltfs glgqqatkaq ylvktvvkar hssfsnqvlf itlpsitgll parqvdrsnm
      541 qlprniqlad pefhkpgkid tllgntlffk llsigqiqls sralilqktl lgwivtgesn
      601 iqtpyraptl vnsfhvttsl dktlnkfwei eefidnqyls aeeraaedlf kqstvrdvdg
      661 rycvklpfne qkqllgnsre iaqkrfyale rklhldqdli vryseflkey edlghmtqvi
      721 vnevtegfyv phhavvkess ettkvrvvfd asaktstgis lndtllngpi lqdtlyklll
      781 rfrsytyvlt adiekmfrqi rvhpddrill vlk
//
LOCUS       XP_034194992             421 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent DNA helicase Pif1-like [Osmia lignaria lignaria].
ACCESSION   XP_034194992
VERSION     XP_034194992.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339101.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194992.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="ATP-dependent DNA helicase Pif1-like"
                     /calculated_mol_wt=46223
     Region          71..336
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(100..106,180)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..421
                     /gene="LOC117611167"
                     /coded_by="XM_034339101.2:1..1266"
                     /db_xref="GeneID:117611167"
ORIGIN      
        1 mlafcqlsdp lnlwkkykds lsedimikva pvrpasakrs ggdlgnrdyl retnydinal
       61 aqalsnnegl ltdeqlavyr qglssvesga ghaffldalg gtgktflinl llakvrsdrg
      121 ivlavassgi aatlleggkt ahaafklpln lmnvetplcn iskqsnmaqv lrdcqlivwd
      181 estmshkrgf ealsttlkdi rgndglmggv tvllagdfrq tlpvvprgtr adevkekpmd
      241 wlcerailtp kndkagvind tllnsfdgve meyrsvdsvv qtddavhypv eflntlnppg
      301 lpahklllkv rapvmllrnl nppklcngtr lrvkvlhrnv ieatvftgca rrdsvlipri
      361 plipseyplh fkrlqfplkv sfaltinkfq vlrgpgelih slvilapkgr ttnvvykevl
      421 k
//
LOCUS       XP_076549193             649 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306631 [Osmia lignaria lignaria].
ACCESSION   XP_076549193
VERSION     XP_076549193.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693078.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="uncharacterized protein LOC143306631"
                     /calculated_mol_wt=73812
     Region          4..533
                     /region_name="Baculo_F"
                     /note="Baculovirus F protein; pfam12259"
                     /db_xref="CDD:371998"
     CDS             1..649
                     /gene="LOC143306631"
                     /coded_by="XM_076693078.1:1215..3164"
                     /db_xref="GeneID:143306631"
ORIGIN      
        1 mgpwylllsi asiqfatanh megdysvtrl rsnpglyaek lapirtfrtt wrlvtaldvt
       61 eiltsrpdti favnrmktmc ksqnitscpm dtlkeeldrk laeavryenl lahalgknav
      121 rvrraslgfi gsiskilfgt ldetdaeyyn reidkiyndq kqitslihnq thviksefrk
      181 aydtlnilsk lattmntrie hvlsktseis kredqlelel amsswgtrli rqtdeyvlml
      241 stltdavmfa klgilhpmil spdqlitacq tiqtstnlef plsmeelkse qlqgtttmsa
      301 vyakgrviit idfpllepns yelyhlypcs sfqqlnants ttlmiqpqtd flaidqatti
      361 ffspdksylk tcknpngrll ceitlplqny eqshiyeidl ilrpehlqwk qcnvkaapan
      421 niqitkleap ntwlfsmqhk eavqikcnkr sagyeqiqga giiqlqshck atidhtrima
      481 sgiktqkyny tfkssfglni pnlipdltph hiqiltklqe nqpkftpini dpdnlltlqe
      541 idnqaqdiaq hhralahtay itygslsavs vvitiiiaai gyryyrkrak nanqprrrqf
      601 ttedikmqnr ptvtpttwps dlqiaespet qqqdgttnpa fktktilyy
//
LOCUS       XP_034195227            1439 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611400 [Osmia lignaria lignaria].
ACCESSION   XP_034195227
VERSION     XP_034195227.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339336.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195227.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1439
                     /product="uncharacterized protein LOC117611400"
                     /calculated_mol_wt=160165
     Region          165..328
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          453..664
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(517..522,557..558,593,595..596,660..661)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(517..522,557,595)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            558
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          681..841
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1062..1112
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1338..1431
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1439
                     /gene="LOC117611400"
                     /coded_by="XM_034339336.2:1..4320"
                     /db_xref="GeneID:117611400"
ORIGIN      
        1 mtdpslhavs ptsskeikpk tkggasakgv nsnvlaaask spparkcpgc sgthglgfcy
       61 kfkalspakr kervqqlkac lsclnvghev gkcpskqvcl acskrhhtll healtappas
      121 apgreggqpa redaastsdd atqqvttlsl svgskavall atakvrleap sgetvevral
      181 ldtgsdtslv sswvaqalrl prravrvais gvkdnevgha tgevslvvrp rhtsdfrlpv
      241 ralvlrklts llpskrivak swphitgltl adpeygvpar vdlllgadvc gtlfgddwrr
      301 gpegtptakl tpfgwvlmgp asddkpkaet aarvlhchge dstsqllqrf weldeirera
      361 pmsqeeekce rhfldthard psgryvvrlp fvkdpltalk snrttalkll fncerrlssd
      421 atlkeqyrnf mmeyltlqhm qgapeeadkg payylphhav rkrhdpsakl rvvfnasfct
      481 atgqslndcl atgqklqadl wmvltrwrlf rvvfttdivk mfrqirvhse dtkwqsilwr
      541 atpdervqdf rlttvtygta capflalrvl aqlandessr fprgasvvrr htyvddiltg
      601 addvsetlal krevvaifka ggfelskwas nipeiqeads sdtrlfqews gistlgvnws
      661 prddafslrv aaaelvrect tkrsilseia glfdplgwaa pvlvvakilm qdlwilganw
      721 dqqlpenvct mwqrfrgslp qldtlkiprw tfassepstq melhgfcdas qrayaaavyl
      781 rvnnngsvtt sllvaktkva pvktitipkl elcgatllsk llirvkegin msgptiawtd
      841 ssvtlhwirg hasqwkpfva hqvsdiqhei padnwrhirs pdnpadlatr gmspaelvds
      901 dlwwhgpkwl teqpefwpat fpesnegiea errgatvhvv kekfgenflc rfsslshlls
      961 viaqcfrfas lsrgqpcekg fvkaeertha flavlrfsqh tsfleeieql qrhgelrkas
     1021 plsscrpfld dqgllrlggr lenaalpyne khpylvakrc plarllirda hyrtlhggpq
     1081 ltrshlmrrv wivrgrslvr seiaacvtca rfsgrragql vgqlpqerta aqraflttgv
     1141 dyagpiklrt spgrghkayk gyialfvcls traihleavs dltsasflaa frrfvgrrgh
     1201 catlfsdngt vfqgadrelr tmfrqstlfy qesaaslald gtcwqfippy aphfgglwea
     1261 gvksvkhhlr rvigdstltf evlttllcqi eaclnsrply alsedpsela vltpghflig
     1321 epttvppepe epctalhtrw rlisqmraqf wkrwrheylq hlqqfpkwrr yrenleigsl
     1381 alirddlqpp akwlmgrvte lhpgpdgcvr vvtlrtekgt vkrpivklcp lpvsptdas
//
LOCUS       XP_034195307            1317 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611466 [Osmia lignaria lignaria].
ACCESSION   XP_034195307
VERSION     XP_034195307.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339416.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195307.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1317
                     /product="uncharacterized protein LOC117611466"
                     /calculated_mol_wt=148282
     Region          <198..263
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          263..454
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(286,340,376,378,425,450..451)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(286,450)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(340,378,451)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          797..1030
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(886..891,921..922,951,1026..1027)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(886..891,921)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            922
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     CDS             1..1317
                     /gene="LOC117611466"
                     /coded_by="XM_034339416.2:1..3954"
                     /db_xref="GeneID:117611466"
ORIGIN      
        1 metqnvgndg ngeqtteqer egafkepgws evrrrrrrsn leeedgvgtt pnkrgtprpn
       61 kgragkipeg vrnvkqtrag nlliefdqga dleglrrnld gnlgpevevt rlqkmmdiei
      121 rgidpsveke evyeaiksel gheargvrvk vfrtdprlsr vaviegpaad msrllngrri
      181 ligwtvvtar eiprllrcyk chaighvasa cnvtgdrdgf crkcgdrghq mrdcknetrc
      241 rlcvadglpp dqaghvaasv rcprlahdll lvgaaerrvd vvavsepytv peswfsdttg
      301 raaiyitgeg lekcrqtsif gsgngyvais yndveivsvy vspnasrqev envfsglell
      361 vkqlsashhr ivvmgdfnak splwgaadwc grgrvlyqla sslglrpvia eggvtcergg
      421 gsvidfllgs eqalsthdas mvldeftasd hryilhefrg dgtglprdqd pfnlgkgriv
      481 edrllasllk rygdenfgkl grtgttnevd kfieelekav nkhtyysrav qsdrkpayww
      541 tesiaemrrl vnksrrkytr arakgneldi atkhedykka rkdmdrevkk akkvnwrell
      601 nevdgdiwgr pykivmkrlk gktnkpdvms pgevetvvkn lfmlkppvar ppersidinv
      661 vneeeigdty lseddsaqgp dvnggadmvr grgmgagsaq gylptylpvv grtsvgasgp
      721 sdppdppefh rnverpdvir paeifllarr msakkapgpd rissavakkf amgaskwlah
      781 ifttcyrrgy wptpwktgrl vllpkakaqg ieekayrpls iisclaklle kvvksrilte
      841 ldkndladnq fgfrrgrstl damkklrkhw etarrvgrhc llilldvkna fntvsyledr
      901 wimldtrdet lwfqvfggvp qgsvigpilw nlvydgllri rlrrdvylig yaddigivii
      961 dddfdrmvgv aertirdmsg wyeseglkla hhkteaillt grkvcggltf rcgdsdirts
     1021 rtarylglil emnrgyrvhi etacnkalky tnilaqlmpn lrgagnmarr lyykvvesvi
     1081 lyaaplwapg lelgvnrkll istqrtallr vaqayrttsa dalcvitgqi pidllvrere
     1141 rvyyrskelg pariegvrdi kkeereatle awqsrwaess kgrwsfalip rlkewigwgp
     1201 kllsfhmtqv vtghgcfgty lqkigkqdrs ecwfcpgevd dpdhflfhcv kwrteraalc
     1261 advgvaldtg nfidslkepr krdriigfvi glmrekeahe rnmerearer rrprstt
//
LOCUS       XP_076549194             469 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611467 [Osmia lignaria lignaria].
ACCESSION   XP_076549194
VERSION     XP_076549194.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693079.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478168) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="uncharacterized protein LOC117611467"
                     /calculated_mol_wt=52081
     Region          1..202
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(7,9,34,88,124,126,199)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(7,34,88,124,126,173,198..199)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(34,198)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..469
                     /gene="LOC117611467"
                     /coded_by="XM_076693079.1:1..1410"
                     /db_xref="GeneID:117611467"
ORIGIN      
        1 mkilqaninh crlahdmllv satekiadlv vvaepytipd nwfsdttgra aifitntgla
       61 kckqttivgc gigyvgisfn glgiisvytt pnanpqevev qfsalealvr dlqsshskil
      121 mlgdfnaksp lwgadrwcgr gkvlfqlats lglhptiavg gatcergggs vvdimlgndl
      181 alsphissev sdeftgsdhr yvwhsfresd svyavdhdlf dlgkgkidee rllaalikky
      241 gdeslerrgr tgtthevdkf iedlerlvnr yttyrratlr ahkpaywwnd avavkrkevn
      301 karrkytrar aknvtadtea ayaiykaakk alnrevnkak ktnwrellse idkdiwgrpy
      361 ktvmkklkgk ttkpdvmcpe eveavveklf ilkppdgqlp vnkgleinsv etagvgylhd
      421 ssylpavrht ngqhlsvsph ihrhtaisdv htapnviafh sndtykyla
//
LOCUS       XP_076549195            1323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306668 [Osmia lignaria lignaria].
ACCESSION   XP_076549195
VERSION     XP_076549195.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693080.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478171) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="uncharacterized protein LOC143306668"
                     /calculated_mol_wt=148857
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          936..987
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1217..1309
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1323
                     /gene="LOC143306668"
                     /coded_by="XM_076693080.1:1..3972"
                     /db_xref="GeneID:143306668"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qaraylsfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifatditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrrt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyegkhpa ilprhsrlse livdhshkat lhggtqltla
      961 hirqtywiig grapvkshil rcvvcarqrg irahqlmgql plsrvtpshp fshtgvdyag
     1021 pialktwkgr gaktqkgwic vfvcmttsav hlemvtdyst dgfvatyrrf aarrgiphti
     1081 ysdcgtnfig adsalksmfv egtqdnqrla klfvddhttw sfnppaaphm ggkweavvks
     1141 vkyhlrrtvs dtlltfeeyy tllnqieatl nsrplepltd dpddlsvltp ahfligrais
     1201 tlpepsveqa nisprarwhl iqqklqqfwk rwstqhlqrm qsiskwhhps nnihvgslvl
     1261 ltderfppck wplarvlavh pgpdgltrvv tiktatstlt rpiaklallp vedqdsspss
     1321 tsc
//
LOCUS       XP_076549196            1736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306707 [Osmia lignaria lignaria].
ACCESSION   XP_076549196
VERSION     XP_076549196.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693081.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478175) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1736
                     /product="uncharacterized protein LOC143306707"
                     /calculated_mol_wt=197357
     Region          136..276
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          441..592
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          725..940
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(789..794,829..830,865,867..868,931..932)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(789..794,829,867)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            830
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          956..1116
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1336..1392
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1434..1531
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1621..1713
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1736
                     /gene="LOC143306707"
                     /coded_by="XM_076693081.1:1..5211"
                     /db_xref="GeneID:143306707"
ORIGIN      
        1 maeqisrlkv kianakrkit nvakfvegyv aerdypvlek rkqdidkeft efenwytele
       61 alvedpthad irteyeekyy aaygklmlfl kdvaapinnl adpiasthsq sivngvntni
      121 vqtskrslpr vnlpsfsgsy eawlgfhdlf kslvdddqni peieklyhlk gclrdeaaev
      181 lasielsaen yrvawsllke rydnrkiirq thvkallnlk hvskefpvrs lldqvqkhvr
      241 aldalkepvd hwntllveii sqklnnvire kweefsgesv sptfkelitf lqrraqfedv
      301 kshqdfvgaq nlsdrkpsha hvkqrsqhay ptatanhscs hcrgkhsihn cetfkklsps
      361 arfeaakkat lcinclrpnh rvmdctgapc rkchkkhntl lhfekqppea tsasepvsss
      421 qsvvnlhaqv ssegllatam vdivnaqgks ktcrafldag sqahfitesv asflrlprks
      481 vdisvsgved isteikhsvs ailksrfsky sktleflvln ritkpmpsia vnqtnldipn
      541 nvvladpkfy dpsqievlig vklfykllcv gqislknhpe avlqktqlgw ivageingss
      601 srnavqchft thstpldatl tkfwelenip svkvlsqeeq aceahfksnt qrnsegryiv
      661 rlpfnenken lgesrsmalr rfhflekrfe knpqlkveyc hfldeykqln hlsllenktl
      721 tmpgfylphh aviredsitt kirvvfdgsa rtssgvslnn almvgptiqd dlftlltrfr
      781 srryaltadi ekmyrqiqvh pddtpyqkil frknpdetvn elsldtvtyg tscapflair
      841 tlhqlandeg aqhpvaasvl kqdfyvddll tgagtrqeaa flrddliall qkggfpirkw
      901 asndpslvpe nsdnsssthm sldpnatikt lgihwnsred iifykvntpn lvktvtkrsi
      961 lsqvaklfdp lgllgpiivk akimiqllwk agvswdesvp adihtmwlef keqlpllvkv
     1021 sfnrltiapn fvkiqmhgfs dasekaygac iymrstdihg nhhvslvcsk srvapvkslt
     1081 lprlelcaal llarlyttvk lalqleinkv ylwsdstitl hwintephll ktfvsnriae
     1141 iqnlsescew rhvptqdnpa dlvsrgqmpq efvdtriwkn gphwlsheen fwpqkkihlv
     1201 eipekraiia apsfananii dddlikdyss lkslkrviay vmrfihnlrn ksqkhtgpis
     1261 atefeastri lvlitqsiaf areiyslkid grvdnksrli plkpfldkqg ilrvggrlih
     1321 selpkeqkhq iilpsdhhit rliireeher lnhagtqatl ysvretfwpl ngrnvtriii
     1381 hkcvrcfrak prsvdyimgd lpqervscsr pfldvgvdyc gplyikekrf rnrnkikvyv
     1441 aifvcmstka vhlelvsdlt teafmaclkr lfsrrgisrt ihsdnatnfv gasrelialh
     1501 kliqsdehnt aiqkflaeqk vtwnfipprs phfgglweaa vrsfkhhlvr tvgdtlltfe
     1561 qletciieie ailnsrplsp mspdpndlqp ltpghfligg pltsfpemdl tdtasnrlsa
     1621 wqhaqklrqh fwrrwqkeyl hqltirskwq snlnhcihpg tlvvikednl pplrwklgri
     1681 vathpgpdgi trvatiktdt geckrcvkkl cplpiecssk adentklcsh nstsnt
//
LOCUS       XP_076549197            1679 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611418 [Osmia lignaria lignaria].
ACCESSION   XP_076549197
VERSION     XP_076549197.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693082.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478175) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1679
                     /product="uncharacterized protein LOC117611418"
                     /calculated_mol_wt=190817
     Region          72..216
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          399..548
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            416..418
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            416
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Region          680..893
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(744..749,784..785,820,822..823,884..885)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(744..749,784,822)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            785
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          908..1068
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1390..1484
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1572..1666
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1679
                     /gene="LOC117611418"
                     /coded_by="XM_076693082.1:1..5040"
                     /db_xref="GeneID:117611418"
ORIGIN      
        1 mgtdseathi aerenfetay fdliarveey ierasgvrsr pleavaggsa tpctvsdaqp
       61 tvklptiqlp sfdgdyndwv rfrdtfmsli dsndsmtkvq kfyylnsalg gsaarvirsl
      121 gvseanykla wdvlkaryen tsalkrhhvn alldlptpqk nsqasvrefi ddatnhlial
      181 ralkvsvdaw dplivpilsr kldiassrew ekrvasvsgd ttfkmfaefl eerstylesv
      241 avnnqvtgnr vetqpqsstt rksprvtshm vsktpscscc qlehalancd kfkkmgvrer
      301 tkhvqesqhc fnclqpghkv qgctrnhcki ckrkhhpllh rddlhsvqsg tskksdvtkl
      361 daatsevtns evtnpevvls cisdqnrslq nailatavvs vqdnigydhe crilldsgsq
      421 anfitdafck klglklcpfe aeiallgsvv klikgkseik irsrhnnfqi svlcysvkki
      481 tddmpnfpiq rgailipsni tladskfees rqidmlvgsg ifwkllcvgq qragpsltwq
      541 kthfgwvlgg iltwptnntq ttnrchlvtn cdlhaqlerf wkveeldsef kntmneceih
      601 ftentrrdst gryivkipfl etinnlgdsr eqaekrlral errfvkqpel rdhyisflre
      661 yeelehmtqi sesnddrpay flphhavske tstttkirvv fdgsakcttg lslndvqrig
      721 pvvqndlvti lirfrihrcv lsaditkmyr qilvdsthrr yqrilwrenp dqpinvyeln
      781 tvtygtasap flatrvlrqv gldnlerypn asrvimddfy vddlltgaet pseirqlkne
      841 lskilgqsgf plrkwatncq fileedsnle vnkelaaekd pktlgliwat kadtlqwsvs
      901 qsdtkrvtkr iilseiakif dplglvgpvi iqakilmqrl wqlqtgwdes vsqelylewl
      961 ryrleleqlv nirvprctiv skynrielhg fsdasqeayg acvylrsemk pgtwevrllc
     1021 aksrvaplrs vsiprlelcg alilvrlikk vkeslpltis verywtdsti viawigsppn
     1081 kwktfvanrv aeiqsitlid nwshissldn padiisrgmt psalvtstlw wsgptwlsrd
     1141 arhwpvsgis ttevpearav kvslavldps kysmfgkyse ysrllrvvaf clrfidrirn
     1201 nasltggatq nrlscleinr arvtifklvq eetfhdelie lkrgemvskr sclyslspfl
     1261 degglirvgg rlvnapikyd krhpivlpak hsfsdlviry ehirllhagc qqvlaslres
     1321 vwlinarnnv rrvvrscvrc frvkpsaiet smgqlpasrv tparpfmtcg vdyagpfltk
     1381 ertrskisvk ayicifvcfa travhielat dlsteallnc frrfiarrgr ckcivsdngt
     1441 nfvgainefr dlkillknkd hnsdiscfll negiewklip phaphfgglw esavksakyh
     1501 ikrvigdqrl tfeelytflt qvesclnsrp isplsvdphd tnpltpghfl igdalcalpd
     1561 tdlrdvavnr lnryqliqqm fqhfwqrwhk eylhqmqqkw kwrttsqqqp avgtlviike
     1621 dnspplrwcl grvvqthkgk dgvirvvtvr tadatykrpi tkicilpied nlkqshvpt
//
LOCUS       XP_076549198             111 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606159 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549198
VERSION     XP_076549198.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693083.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..111
                     /product="uncharacterized protein LOC117606159 isoform X1"
                     /calculated_mol_wt=13399
     CDS             1..111
                     /gene="LOC117606159"
                     /coded_by="XM_076693083.1:496..831"
                     /db_xref="GeneID:117606159"
ORIGIN      
        1 mrkfflrclh amrqrlrlll rrrflafgdk cdwketkfrn ralifpgrrf yaddsdeviv
       61 swvaldnaak fpsarrkilv akqdkltdls eilrrlpfwa lvqrmqeawn w
//
LOCUS       XP_076549199             111 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606159 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549199
VERSION     XP_076549199.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693084.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..111
                     /product="uncharacterized protein LOC117606159 isoform X1"
                     /calculated_mol_wt=13399
     CDS             1..111
                     /gene="LOC117606159"
                     /coded_by="XM_076693084.1:496..831"
                     /db_xref="GeneID:117606159"
ORIGIN      
        1 mrkfflrclh amrqrlrlll rrrflafgdk cdwketkfrn ralifpgrrf yaddsdeviv
       61 swvaldnaak fpsarrkilv akqdkltdls eilrrlpfwa lvqrmqeawn w
//
LOCUS       XP_076549200             105 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606159 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549200
VERSION     XP_076549200.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693085.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="uncharacterized protein LOC117606159 isoform X2"
                     /calculated_mol_wt=12591
     CDS             1..105
                     /gene="LOC117606159"
                     /coded_by="XM_076693085.1:496..813"
                     /db_xref="GeneID:117606159"
ORIGIN      
        1 mrkfflrclh amrqrlrfla fgdkcdwket kfrnralifp grrfyaddsd evivswvald
       61 naakfpsarr kilvakqdkl tdlseilrrl pfwalvqrmq eawnw
//
LOCUS       XP_076549201             230 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609875 [Osmia lignaria lignaria].
ACCESSION   XP_076549201
VERSION     XP_076549201.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693086.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="uncharacterized protein LOC117609875"
                     /calculated_mol_wt=26669
     Region          11..98
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     CDS             1..230
                     /gene="LOC117609875"
                     /coded_by="XM_076693086.1:781..1473"
                     /db_xref="GeneID:117609875"
ORIGIN      
        1 mdfskaeilt lievyrsksv lwdpkncdyf nklkkedvwe elsaeiekpk aickkkieyl
       61 laglrreklk mkrsvgtgkg tqevyksqwf afesmqflwd knksrrtlst medevqivsp
      121 eavedevteq symgqnveml ggepgpstst plrptrtvaa krrhtekedr lertfeilet
      181 asdpppdesq hfanfiaskl rklnnyervh iearimqlfv eieptcidni
//
LOCUS       XP_076549202             390 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306711 [Osmia lignaria lignaria].
ACCESSION   XP_076549202
VERSION     XP_076549202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693087.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="uncharacterized protein LOC143306711"
                     /calculated_mol_wt=43691
     Region          313..>353
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..390
                     /gene="LOC143306711"
                     /coded_by="XM_076693087.1:12..1184"
                     /db_xref="GeneID:143306711"
ORIGIN      
        1 mvpgmkttgt dhdevpvpvp ekekekkrqy kdmatqtade rdmdfppllt srhkrkeitp
       61 ptenagkktr fflsstegtp egntrrrkge detpredrgp sdqewelvqa rrrrndkern
      121 kdrgeeerkk mdgksrgdsv gvkgtpkrrp gpakpqaiav rfkkdtsfad ilrkvkgaag
      181 sspegvktvk qtragnllie ftpgadldgf krkidgklgp dvevarlqqk idvelrgidp
      241 svekedvvea lskelgheas dirikvlrvd prlnkiavie gpatdmvkli rkgrikigwt
      301 ivlvkeiprl lrcfrchelg hvatnckssk egggfcrkcg elghqmrecg kaprcrlcva
      361 dklpeemvgh vaasvrcqry kdalkikrqs
//
LOCUS       XP_076549203            2171 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306712 [Osmia lignaria lignaria].
ACCESSION   XP_076549203
VERSION     XP_076549203.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693088.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 48% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2171
                     /product="uncharacterized protein LOC143306712"
                     /calculated_mol_wt=247298
     Region          287..468
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(355..360,389..390,417,419..420,464..465)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(355..360,389,419)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            390
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          562..681
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(566..569,602..604,607,632,665,679)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     Region          1203..1260
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          <1259..1429
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     Region          1531..>2023
                     /region_name="Baculo_F"
                     /note="Baculovirus F protein; pfam12259"
                     /db_xref="CDD:371998"
     CDS             1..2171
                     /gene="LOC143306712"
                     /coded_by="XM_076693088.1:1..6516"
                     /db_xref="GeneID:143306712"
ORIGIN      
        1 mcwptkvssd sfpqtkskvv mmmtallarq ytndfcvasr sapndscvlv rydeiintry
       61 ylrqeqaeis fahntlvtks epikpfrfig enppkekprh ykprvyriqs rsrqaiaidt
      121 idcdlkegyl pllqtkdgiy igeaavsvnq gvchvlaint lnqdveieip pqeilpfkyh
      181 nfpeekehpn ldsehenrse pldrvteilt tlrldhvnne ekehvielir efpeifhlpg
      241 dklkstpliq hpihtiddvp intrqyrfpp ihqaeierqi ttklaagiit psnspynspl
      301 wivpkkpdsk gnprwrmvid frklnektig dayplpnivd ildrlgeary fstfdlasgf
      361 qqiemdpkhr sktafttphg hyeyirmpeg lknapatfqr lmdqvlrglq giealvyldd
      421 ivvyaknlve hgkkirrlft rlgearlslq pdkceflkte vtylghiiss kgvkpdkkki
      481 iavknfprpq naknikqflg lagyyrrfik dfaakakplt ellkknskfi wgkeqkqsfk
      541 tlrkalcksp ilqypnfdkp ftlttdasdf aigavlsqdv dghdlpvayl srslikaein
      601 ysttekecla vlyavlhfrp ylygrkftlv sdheplkwid sikdpgqrlv rwrlklrdye
      661 ydfvhkpghl ninadalsrn pikelhdtss dsssketsds esecekilpi rkptvkpkvt
      721 ipsasrikpt veprrpvgrp pgrktqpkld kptldksais trtrsksehe gspktinyae
      781 pstseisqnt dedeesiets dlpvfrgrks vlpslgpptv spqnlprktv hlekpenipd
      841 aepdndkdet tethpdnimi eipapldnsd devflpktie qppinldelh esfdrfdksl
      901 herseilqes tdkaepeiee ldeeelnrrv dkllaryeqd eqdatkqtti rptskeftld
      961 tiheenelna ptefentvvi rqpyrsilpt plsldkplal stpfpqttns etksnpsflp
     1021 krlitsqpyd tskiiqyrpg iqitesilpd iepehpsrkt ikftrenlts trshyvhfip
     1081 adielttpis rllvdmgvis ianlkaanpk lgqvlvtprg kykifsvvvk qkhydvisis
     1141 nfrialhnlr silsklkint irisrsgdft dtlpqgtlvn vlrdtlgdsd vevtictgsi
     1201 qvppeelrlk iiselhgslv gghkgvtkty hrirerfhwp gmrndvqnfi rtcekcqenk
     1261 lvrtktrepm litdtplqpf dkisldtvgp lpvtpsgnrh iltiqdnltk yciavavpni
     1321 kaetiahalv thvilqygts rtiltdqgts finrvlkeia kvfkinhvtt sgyrpqtngs
     1381 lershivlte yikhylddfd dwdkivpfam fsyntsvhea tnftpyelif gkiarcpsaf
     1441 pseeslptyn nylqelisrl temqtiagqn liqakekskl yydekvqpfq gkigdmarvl
     1501 vepqkgklgn ryhgpykivg ileknngkmk lvtfisltlt lwsgshsaeq kktyeitkld
     1561 atpgmyfekl hnirliaadw rvtlsidtnk lktqfpleys eldgaygkcl slttatacaa
     1621 tlrlphvygk adqalslqde lneivtefqt pesydgpkti krrsapfgfi gsishtlfgt
     1681 ltsedaeyyn seidklyhnq nelahimknq thivrnglhk fhqileknse lisenrnrln
     1741 efaeaiqree dsvttlqymm alsrfvhgie isldhyikta etylavirav ksgevphell
     1801 drsisrriis dirntqrnfd lpippghqsl keltgsttvn aiiyqgriii vlqlplsdgt
     1861 tyhlyrtysw pipqrlgqdt tasgyiqpks pylaisndhr fyfeadesyi nnchktpyth
     1921 lcplgrplse iasstacevt lllrptthaf ktcevrlqpq htpfwahlrt mqawlyslek
     1981 pesvqlicng mkddiitieg tgifqlragc iaktthetli gmqhlesleq yfyqpnllln
     2041 isefspqihr ykeikpltmp skasgvstpi ddsltdieak laemdthhrs rnlttnvlhg
     2101 glaylailti ailvyctrht tkkiwitkic qkrrtepenq stppeqpptt tppsiaqqpl
     2161 pplpqqralv n
//
LOCUS       XP_076549204            1736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306713 [Osmia lignaria lignaria].
ACCESSION   XP_076549204
VERSION     XP_076549204.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693089.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 62% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1736
                     /product="uncharacterized protein LOC143306713"
                     /calculated_mol_wt=197355
     Region          136..276
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          441..592
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          725..940
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(789..794,829..830,865,867..868,931..932)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(789..794,829,867)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            830
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          956..1116
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1336..1392
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1434..1531
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1621..1713
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1736
                     /gene="LOC143306713"
                     /coded_by="XM_076693089.1:495..5705"
                     /db_xref="GeneID:143306713"
ORIGIN      
        1 maeqisrlkv kianakrkit nvakfvegyv aerdypvlek rkqdidkeft efenwytele
       61 alvedpthad irteyeekyy aaygklmlfl kdvaapinnl adpiasthsq sivngvntni
      121 vqtskrslpr vnlpsfsgsy eawlgfhdlf kslvdddqni peieklyhlk gclrdeaaev
      181 lasielsaen yrvawsllke rydnrkiirq thvkallnlk hvskefpvrs lldqvqkhvr
      241 aldalkepvd hwntllveii sqklnnvire kweefsgesv sptfkelitf lqrraqfedv
      301 kshqdfvgaq nlsdrkpsha hvkqrsqhay ptatanhscs hcrgkhsihn cetfkklsps
      361 arfeaakkat lcinclrpnh rvmdctgapc rkchkkhntl lhfekqppea tsasepvsss
      421 qsvvnlhaqv ssegllatam vdivnaqgks ktcrafldag sqahfitesv asflrlprks
      481 vdisvsgved isteikhsvs ailksrfsky sktleflvln ritkpmpsia vnqtnldipn
      541 nvvladpkfy dpsqievlig vklfykllcv gqislknhpe avlqktqlgw ivageingss
      601 srnavqchft thstpldatl tkfwelenip svkvlsqeeq aceahfksnt qrnsegryiv
      661 rlpfnenken lgesrsmalr rfhflekrfe knpqlkveyc hfldeykqln hlsllenktl
      721 tmpgfylphh aviredsitt kirvvfdgsa rtssgvslnn almvgptiqd dlftlltrfr
      781 srryaltadi ekmyrqiqvh pddtpyqkil frknpdetvn elsldtvtyg tscapflair
      841 tlhqlandeg aqhpvaasvl kqdfyvddll tgagtrqeaa flrddliall qkggfpirkw
      901 asndpslvpe nsdnsssthm sldpnatikt lgihwnsred iifykvntpn lvktvtkrsi
      961 lsqvaklfdp lgllgpiivk akimiqllwk agvswdesvp adihtmwlef keqlpllvkv
     1021 sfnrltiapn fvkiqmhgfs dasekaygac iymrstdihg nhhvslvcsk srvapvkslt
     1081 lprlelcaal llarlyttvk lalqleinkv ylwsdstitl hwintephll ktfvsnriae
     1141 iqnlsescew rhvptqdnpa dlvsrgqmpq efvdtriwkn gphwlsheen fwpqkkihlv
     1201 eipekraiia apsfananii dddlikdyss lkslkrviay vmrfihnlrn ksqkhtgpis
     1261 atefeastri lvlitqsiaf areiyslkid grvdnksrli plkpfldkqg ilrvggrlih
     1321 selpkeqkhq iilpsdhhit rliireeher lnhagtqatl ysvretfwpl ngrnvtriii
     1381 hkcvrcfrak prsvdyimgd lpqervscsr pfldvgvdyc gplyikekrf rnrnkikvyv
     1441 aifvcmstka vhlelvsdlt teafmaclkr lfsrrgisrt ihsdnatnfv gssrelialh
     1501 kliqsdehnt aiqkflaeqk vtwnfipprs phfgglweaa vrsfkhhlvr tvgdtlltfe
     1561 qletciieie ailnsrplsp ispdpndlqp ltpghfligg pltsfpemdl tdtasnrlsa
     1621 wqhaqklrqh fwrrwqkeyl hqltirskwq snlnhcihpg tlvvikednl pplrwklgri
     1681 vathpgpdgi trvatiktdt geckrcvkkl cplpiecssk adentklcsh nstsnt
//
LOCUS       XP_076549205             789 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306714 [Osmia lignaria lignaria].
ACCESSION   XP_076549205
VERSION     XP_076549205.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693090.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 44% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..789
                     /product="uncharacterized protein LOC143306714"
                     /calculated_mol_wt=87997
     Region          6..204
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(10,12,37,93,126,128,202)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(10,37,93,126,128,176,201..202)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(37,201)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          <413..>479
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          553..>760
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(642..647,701..702,731,733..734)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(642..647,701,733)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            702
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     CDS             1..789
                     /gene="LOC143306714"
                     /coded_by="XM_076693090.1:2479..4848"
                     /db_xref="GeneID:143306714"
ORIGIN      
        1 msaftvlqan lnhcraaqdi lwqaahdyra divvisephr prpewhtdps gtlsmwcvqr
       61 tpptaelgmi rkihtsdgci gletrdfvif gcyfspsltm atyegqldgl aagvasstkp
      121 viisgdfnar svqwgsdrtd argyrllrvc eqsglipvns kggpsfvrnh qqsridavac
      181 shalrsrlks sfvstkytgs dhnylvhrfs atdtfesssm nsmwntsynp pdkeawasii
      241 knnlslppet dawvrelpih vfedtasidt aiaeleswye sariprshns arkrkyvaww
      301 tkeisrlrln aarsrakwrr aalnmsknnr nvsvdsqhpl rqlmvsdrra likairqskd
      361 aswsgmidei arypwgkpyk avmnrlkgrs pllrlnetef ktavsqlfvt eppvttagsa
      421 pvpdvtnptp aprnrsaspq pgpsrttpsr stsigpaapp svnrpitnst sptpsftepi
      481 crvapedpri pypfstrdav aalakvsggk apgrdgitge alrpimrlap ewirdlfnac
      541 yrlgyfpnsw kvgrlvlipk pgkpfssysd lrplcmlsnl gkgfeyalre lleeavqnsg
      601 dlspwqfgfr kkrstmqamk vvisewnrar sygnhcllig ldvknafntv rweniilaaq
      661 rrhfpphlia mlqnylnsrf igttmpdgtw ieqevfagvp qgsvlgpflw nlaydsilev
      721 vlprnamtia faddlaliil aalsqireia eealrrlsvw hmnlkqdpgg lasngmaesh
      781 kpsissmwy
//
LOCUS       XP_076549206            1663 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611395 [Osmia lignaria lignaria].
ACCESSION   XP_076549206
VERSION     XP_076549206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693091.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1663
                     /product="uncharacterized protein LOC117611395"
                     /calculated_mol_wt=186477
     Region          <521..589
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          593..789
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(593,595,620,674,710,712,785)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(593,620,674,710,712,759,784..785)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(620,784)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          1136..1392
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(1225..1230,1283..1284,1313,1315..1316,1388..1389)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(1225..1230,1283,1315)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            1284
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     CDS             1..1663
                     /gene="LOC117611395"
                     /coded_by="XM_076693091.1:1..4992"
                     /db_xref="GeneID:117611395"
ORIGIN      
        1 mdfsnykfnm ehvkeskkty gaggigppst gaagslcvgs paslssdyti rvvdrqapvk
       61 vtatgeasas nqgsadekra fvrkprvsrt ppsihkdsvf kaprkrlesv dltgdesetd
      121 gdsdvsrrla rgrkigkppp qqrkesltle ekvdaslavl ervlnvindh navfdnlilk
      181 kggdtdcalr styvsfkdni revmlavtdl rmahtdhtrs gknsdtyqmk tktmmtpggm
      241 sveqppekek ktiatqtesd grgdfpplpn rqkrkeitpp tdnqgkrtrf fntqsetpre
      301 rrpvdrykgr vqdvgedsge gewaeirrrr rktnledsdr ketvtpnrns rginanergg
      361 gsknmprrav kpqaiavrfg ketsfaevlk kvkgvtgrvp dgvrnvkqtr agnlliefap
      421 gsdldelrkn lvgklgvnve vaklqtmmdv emrgidpsve keevleaira elgheakgvr
      481 vkvlrtdprm skvavvegpa admqrilran rimvgwtvis areiprllrc ykchgighia
      541 ggcklvvgdg glcrkcgavg hhmkdcsnep kcrlcvedgl aesqtghvaa svnlnhcrla
      601 hdllavsare qeadilvlse pyltpdnwft dltgrasisi tttgiaksrk ltvlscdkgf
      661 vcinyndici msvyaspnds sqafeaqlal memflagikg tltkvivagd fnaksplwgs
      721 kkwcsggrtl yqwcnglglt ptiakggvtc drgggsvvdl lfcsndamkl hvtsdvldiy
      781 tasdhryivq kfgraktdsm rekdpfdlgk pkideaglle elirkygdes ydkraktgtt
      841 levdrfiedv drlvnkytsy rgpykgnktp vywwnedtar ckkevnkarr rhtrlrakgn
      901 erdieeaykv ykkarktmkr avekakktsw knmltdidkd iwgkpykvvt wklkgkgskp
      961 dilsaeevgs vvkklfilsp prpdtpeknt eivvrtvene deviylpder gctvapeken
     1021 nndkngerrg tgylpgglre iperetesre reinveeelp dvpvpvpvtl ehrrvggitp
     1081 eeitsvvrcl pvnkapgpdr mptsiakkfs lraarwlayi yntcyargyw prswktgrlv
     1141 llpkgkatra derayrplsv isclakilek lvktrilrel enndladnqy gfrkgrstld
     1201 amdkvdgiwq karkdgrhcl lilldvknaf ntirwdsvir cleergfape lvnlirsyld
     1261 erwivydaae gelnfqvfgg vpqgsvigpi lwnlvydgll rvrlrrdvhl vayaddigiv
     1321 ivdvdlarmv hvaqetikdl gvwyksegls lahhkteail ltgrkvvggl svicgdaeir
     1381 tartarylgl ilgmnhgfks hietacsral kyagvlahlm pnlggagnla rrlfyrvves
     1441 vilyaaplwv ggldfrtnck llystqrtal lrvaqayrtt swdalcvitg qipidllvke
     1501 rariyekrke nrelsieeiv rikkeerevt mdtwesrwta stkgrwtfql ipnirewvtw
     1561 gpkvlsyhvt qvltghgcfg syllrigkqe naccwfcegh ednpehfllh csrwrekrat
     1621 mnatigenlg vgnfirlfgd srsreqiimf atsspvlisl fqi
//
LOCUS       XP_076549207             355 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306715 [Osmia lignaria lignaria].
ACCESSION   XP_076549207
VERSION     XP_076549207.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693092.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="uncharacterized protein LOC143306715"
                     /calculated_mol_wt=39512
     Region          1..203
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(7,9,34,88,124,126,199)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(7,34,88,124,126,173,198..199)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(34,198)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..355
                     /gene="LOC143306715"
                     /coded_by="XM_076693092.1:1..1068"
                     /db_xref="GeneID:143306715"
ORIGIN      
        1 mnilqaninh crlahdmliv raseqmvdvv vvaepytvpe swfvdttgra aiyitkvgma
       61 kckqttiigc gngfvgisyn gvgiisvyis pnvshqeven qfsalellvk dmytahskif
      121 vigdfnaksp lwgsvtwcgr gralfqlvts lglrpiiaeg gvtcergagt vidillgsdr
      181 alsshassvi seeftasdhr hivhafkdkd panpeaqdif dlgkgridee glltallkry
      241 gdenfgkwgk tgttlevdtf iedvekmvnk ythyrrtmik nrkpaywwtd avatkrkevn
      301 ksrrkftrak akgitedtka aheiykiakk amdrevknak kanwkellse idrda
//
LOCUS       XP_076549208             469 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306716 [Osmia lignaria lignaria].
ACCESSION   XP_076549208
VERSION     XP_076549208.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693093.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 51% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="uncharacterized protein LOC143306716"
                     /calculated_mol_wt=54207
     Region          3..61
                     /region_name="FLYWCH"
                     /note="FLYWCH zinc finger domain; pfam04500"
                     /db_xref="CDD:461332"
     Region          <210..277
                     /region_name="MULE"
                     /note="MULE transposase domain; pfam10551"
                     /db_xref="CDD:463145"
     CDS             1..469
                     /gene="LOC143306716"
                     /coded_by="XM_076693093.1:1..1410"
                     /db_xref="GeneID:143306716"
ORIGIN      
        1 mqfidsekgq trllyngfly yqdrtvaest ywrccnyqkl rctgrvisvk dsvkkenahn
       61 hpgdplfvnk ekgvnemkhe sststsgihn ivvigvgsvs dniaavmpki sslkrtlrrv
      121 rnkihedpri pvclkelsdi pdnlryterg kvflsfdsgv ddperilift trknlkilat
      181 tdhwfgdgpf dncpvqfaqi ytihglfdst vvplvyallp rktkgtycrf lgalknlksv
      241 lcpstfmidm eiafvgsind vfaganvrfc yfhfcqaiyr klsnyhlkir ydtdenfcit
      301 iktlmalafv pvndvvklfe elvcinytgk denvyeffty fekeyigtvk lmkirelprf
      361 qiscwnqyda ilndlprtnn nvegwhrgfn elvakqnasi whilrclkme qaknefmisq
      421 irsgaevskp vkkfiryrdq lksvvnryns siyeekidyv yavarillv
//
LOCUS       XP_076549209             232 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306717 [Osmia lignaria lignaria].
ACCESSION   XP_076549209
VERSION     XP_076549209.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693094.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..232
                     /product="uncharacterized protein LOC143306717"
                     /calculated_mol_wt=25688
     Region          137..219
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..232
                     /gene="LOC143306717"
                     /coded_by="XM_076693094.1:1..699"
                     /db_xref="GeneID:143306717"
ORIGIN      
        1 mlsdngtvfh gadrelrnmf rgasafylna taslaaegtd wsfippyaph fgglweagvr
       61 tvkhhlrriv enatltfeem ttllcqveac vnsrplhpls ddpsdlsalt pghfligept
      121 civpdpesds hdislisrwr qisalrshfw hrwrreylqk enlklgtlvl lrdelqppak
      181 wpmgrvealh pgpdgrvrva tlkttsgsvt rpivklcplp vpsadvtspt st
//
LOCUS       XP_076549210             333 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306718 [Osmia lignaria lignaria].
ACCESSION   XP_076549210
VERSION     XP_076549210.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693095.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="uncharacterized protein LOC143306718"
                     /calculated_mol_wt=37110
     Region          <1..144
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            38
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          161..320
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..333
                     /gene="LOC143306718"
                     /coded_by="XM_076693095.1:1..1002"
                     /db_xref="GeneID:143306718"
ORIGIN      
        1 mfrqirvdpa dadwtrilwr srpdqpvqdf rlktvtygta capflalrvl vqlaadeeer
       61 fplravairr hsyvddilag addeeaalel rrqvisilqs ggfdlskwas nkpslqeaee
      121 sqacvfqdht gvstlgvvwr pttdtfslrv lpplkepsry tkrrilseva slfdplgwaa
      181 pvlifakilm qdlwiigaew dedlpdqlls awktfrktls qfdalaiprw tnysadsaql
      241 dlhgfcdasq rayaavvylr vsrgdvsvtt llvaktkvap vkavsiprle lcgavllskl
      301 iaevqrglnv patitawtds svtlhwirgh ast
//
LOCUS       XP_076549211             351 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306719 [Osmia lignaria lignaria].
ACCESSION   XP_076549211
VERSION     XP_076549211.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693096.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..351
                     /product="uncharacterized protein LOC143306719"
                     /calculated_mol_wt=38210
     Region          53..>176
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     CDS             1..351
                     /gene="LOC143306719"
                     /coded_by="XM_076693096.1:1..1056"
                     /db_xref="GeneID:143306719"
ORIGIN      
        1 manpaksapt akgdaptspp pavfsdteek nvaacfacsa ttrpaallat akvrveapsg
       61 ehlevralld tgsdtslvsg wvaqalrlpr ravsvaisgv qenesglaks evsfslrsrl
      121 dpsfhlsvra lvlrkltall paravqpqsw phlqgvilad peygvpsrvd lilgadicgv
      181 llqdesrvgp igtptarrtp lgwvlmgpvs dakgpsskav vhslhcqtdd stnrllrrfw
      241 eleelheqpp lspedrecer hfrethyrde tgrysvhlpf kldpslalkp srstalklll
      301 scerklasne awrdkysqfm deyaalshme avpesavqdp ayylphhava k
//
LOCUS       XP_076549214             627 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306720 [Osmia lignaria lignaria].
ACCESSION   XP_076549214
VERSION     XP_076549214.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693099.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="uncharacterized protein LOC143306720"
                     /calculated_mol_wt=70421
     Region          202..420
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(267..272,307..308,343,345..346,411..412)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(267..272,307,345)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            308
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          435..595
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..627
                     /gene="LOC143306720"
                     /coded_by="XM_076693099.1:1..1884"
                     /db_xref="GeneID:143306720"
ORIGIN      
        1 mhfltryvps qkgaitnwsh laelnladed pmsadpidvi igadlygqll lpgvkkgsay
       61 tptaqntvlg wilsgpvqss ihvsvgihhi svledldqdl rrfweieals spilmtpedk
      121 ncelrasras ngryivrlpf ktkppaklge srpgalqsfc alerrlsrdt atlrmyrefl
      181 seyasfghmk kadplppitr dcyyiphhav fressettrl rvvfnasrrs sngkslndll
      241 ltgpklqqvl vailirwrqf qfvytadvek myrqilidpr dldyqrilrr dspllpveey
      301 qlltvtygta papylalrvl qqlaededsp ypeavsvlre qsyvddflsg gdslesarql
      361 rdqtvellgk ggfslrkwas nssallsdin pqnhglatsk slkadeiisa lgitwnpald
      421 qfhfklavpt elpktkrtil stiaklfdpl gwiapviiva kiylqtlwss qcdwddvlps
      481 sllskwhnfy dqlpdlehis iprkiypqii ktqslhgfsd astkayaaav ysrsvlcdgt
      541 vsislilakt rvaplktisv prlelcaall larllkfvri alnqetiesh cwtdssvtle
      601 wvnqpptrwk tfvanrvali helspna
//
LOCUS       XP_076549215             270 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306721 [Osmia lignaria lignaria].
ACCESSION   XP_076549215
VERSION     XP_076549215.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693100.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="uncharacterized protein LOC143306721"
                     /calculated_mol_wt=30036
     Region          164..256
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..270
                     /gene="LOC143306721"
                     /coded_by="XM_076693100.1:1..813"
                     /db_xref="GeneID:143306721"
ORIGIN      
        1 mvtdyttdgf iatyrrfvar rgipstifsd cgtnfigans alksmfvegt qdnqrlaklf
       61 vnvqttwnin platphmggk weavvksiky hlrrtvsdtl ltyeeyttll tqieatlnsr
      121 plgpltddpd dlsvltpahf ligrpistlp epsadhaqls prawwhliqq klqqfwkhws
      181 sqhlqrmqsi skwhhpsnni nvgslvlltd ecfppckwpl apvltvhpgp dgltrvvtik
      241 tatttltrpi aklallpvtd tnsspsstsc
//
LOCUS       XP_076549216             445 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306722 [Osmia lignaria lignaria].
ACCESSION   XP_076549216
VERSION     XP_076549216.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693101.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478176) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="uncharacterized protein LOC143306722"
                     /calculated_mol_wt=50727
     CDS             1..445
                     /gene="LOC143306722"
                     /coded_by="XM_076693101.1:1..1338"
                     /db_xref="GeneID:143306722"
ORIGIN      
        1 mrllgvardg inlfanlmdi cdglsdasyn sivkhmhsat ktifefcckk aveeeknene
       61 krkkqllnlk vsgdgswkkr gfkslygvtt ligyysgkvi dlvvkssycq achmwksren
      121 teeyhewyeh heeecaknhe gsagkmevsa itemflksee kygvkygnyi gdgdsktfka
      181 ildvnpygde lqvkksecig hvekrmgtrl rnvkkinklg gkgkltdvli kklqsyyrla
      241 irrnvnsvid mknaimatyy hlcstnenpr hehcptgees wckwkkaeal gstdnlnhpl
      301 plhpdvqkhi lpifeelsne dllhrclggh tqnanesfns tvwrlapkhl hsglkileia
      361 sfiaagmfne gyfsilkimq elnltigkqa mdyaeksnqr riqrqerrss lstkkarkar
      421 reqlaeqndy feeaegllyg agiad
//
LOCUS       XP_076549217            1323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306777 [Osmia lignaria lignaria].
ACCESSION   XP_076549217
VERSION     XP_076549217.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693102.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478177) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="uncharacterized protein LOC143306777"
                     /calculated_mol_wt=148830
     Region          44..183
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(49,51,53,76..78,117)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            49..51
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            49
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(76..79,83..86)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          321..537
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(386..391,425..426,461,463..464,533..534)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(386..391,425,463)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            426
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          552..713
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <680..755
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     Region          936..987
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1217..1309
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1323
                     /gene="LOC143306777"
                     /coded_by="XM_076693102.1:1..3972"
                     /db_xref="GeneID:143306777"
ORIGIN      
        1 mgqrssttan tndqrsssla tqaeltllat ahaqvitsst tynarllvds gselsfvsed
       61 lveqlglqrt qssisitgig gkkstqtrgi vslhlqslyn tssisiqthv lrtlttilps
      121 seaphqdwph lnrlkladpe fykprpidvi igadnygrii ksnvikgsps mpvaqlsifg
      181 wlvigparrr qarayssfna sipqdsdaal relltkfwiq eelptentsq ltpdeqeced
      241 hfrathsrds tgryvvripl ktntkvlgts ystarsclrr tlskltrnpe yreqyqrfmm
      301 eyedlghmkk assispndss iyylphhgvf kpgsettklr vvfngssptt tglsvndimh
      361 tganlllnin dvllwirhhk cifatditkm yrqvrvhedd wnlqrilwid eesnevpytl
      421 ttvtygtkaa pflavrallq laedegqryp lavpsikyar yvddifggad sldslieias
      481 qltdlcnagg fplakwhsne aslletivpc nnsqgtsisl ddcntkilgl rwntvndsft
      541 fttkshhnln ftkrlvlsev aqifdplgfl spviirakvl lqelwllnlk wddplpshvt
      601 irwiairedl tslarlsipr wfntynnstv elhgfsdasq lamaaviyit vfspstdhkv
      661 tslvcsktkv aplkrltipr leltaalila kllkyvqanl klniatihlw tdsqvtltwi
      721 kshasrwkdy vrnrvtqiqe ltqtatwvhv pgtsnpadca srgltttqle qhelwwrgpp
      781 wllqsqeswp vqrnasddtc lqecrpgish vltcqkidyh wdlihkyssl qklfhvtsvc
      841 yrfisklrrt tastrlsntp adlerakifw ikatqsayfs helkmmvnnq tlpsshafnr
      901 ltafiddqgv irvggrlsna qltyegkhpa ilprhsrlse livdhshkat lhggtqltla
      961 hirqtywiig grapvkshil rcvvcarqrg irahqlmgql plsrvtpshp fshtgvdyag
     1021 pialktwkgr gaktqkgwic vfvcmttsav hlemvtdyst dgfvatyrrf aarrgiphti
     1081 ysdcgtnfig adsalksmfv egtqdnqrla klfvddhttw sfnppaaphm ggkweavvks
     1141 vkyhlrrtvs dtlltfeeyy tllnqieatl nsrplepltd dpddlsvltp ahfligrais
     1201 tlpepsveqa nisprarwhl iqqklqqfwk rwstqhlqrm qsiskwhhps nnihvgslvl
     1261 ltderfppck wplarvlavh pgpdgltrvv tiktatstlt rpiaklallp vedqdsspss
     1321 tsc
//
LOCUS       XP_034195151             300 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611317 [Osmia lignaria lignaria].
ACCESSION   XP_034195151
VERSION     XP_034195151.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339260.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478177) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195151.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="uncharacterized protein LOC117611317"
                     /calculated_mol_wt=34150
     Region          <1..122
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(7,45,118)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..300
                     /gene="LOC117611317"
                     /coded_by="XM_034339260.2:1..903"
                     /db_xref="GeneID:117611317"
ORIGIN      
        1 mymasvyasp ngtvqeyedm ldslaaemnt lkgstdekiv mgdfnakspl wcadawcsrg
       61 rklfefcnva elfpvvtkgg atcdrgrgik idimscnrra lqdltgsvfi edysasdhky
      121 vvhtfqlaqh kgetaspfei ekgkldeegf veaflkkfgd knfdkrprth ttlevykfle
      181 emselvnryt ryggarskrt pvpwwnsdia ekrklaqkas rrltrvragi dheaiekstr
      241 aykkckmdln teirkakrns wanllqevdk dvwgrpyklv mravkqtssv sakggpqwtr
//
LOCUS       XP_034195153             315 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611318 [Osmia lignaria lignaria].
ACCESSION   XP_034195153
VERSION     XP_034195153.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339262.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478177) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195153.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="uncharacterized protein LOC117611318"
                     /calculated_mol_wt=35910
     Region          <90..252
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..315
                     /gene="LOC117611318"
                     /coded_by="XM_034339262.2:1..948"
                     /db_xref="GeneID:117611318"
ORIGIN      
        1 mgyrwkksts irkilvekpn ivawrdkylt aidsyrkqgr niiylvdntl sfskcwqsde
       61 emgilknnss shryiivhag geegfvngva lifkarsttg dyhgqmnaln fekwinekll
      121 pniplhsiiv mdnapyhtvq inkpptaygk ksdmikwlge nniqhaatmk kyqlveliqq
      181 nksldktykv delfkrhghn vlrlppymce lnpielawak vkriirennt stltaveltn
      241 ltekaindvs tedwvgfckh vqkiekqyve rdnamesime eivdtrpmna tsctdssdsd
      301 tnssssssss snvqd
//
LOCUS       XP_076549218             771 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611475 [Osmia lignaria lignaria].
ACCESSION   XP_076549218
VERSION     XP_076549218.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693103.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478177) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..771
                     /product="uncharacterized protein LOC117611475"
                     /calculated_mol_wt=86061
     Region          1..174
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Region          534..760
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(623..628,681..682,711,713..714)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(623..628,681,713)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            682
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     CDS             1..771
                     /gene="LOC117611475"
                     /coded_by="XM_076693103.1:1..2316"
                     /db_xref="GeneID:117611475"
ORIGIN      
        1 mkilqaninh crlahdmllv satekiadlv vvaepytipd nwfsdttgra aifitntgld
       61 kckqttivgc gigyvgisfn glgiisvyvs pnanpqevev qfsalealvr dlqsshskil
      121 mlgdfnaksp lwgadrwcgr gkvlfqlats lglhptiavg gatcergggs vvdimlgndl
      181 alsphissee rlvaalikky gdesfdrrgr tgtthevdkf iedlerlvng yttyrratlr
      241 ahkpaywwnd avavkrkevn karrkytrar aknvtadtea ayaiykaakk alnrevnkak
      301 ktnwrellse idkdiwgrpy ktvmkklkgk ttkpdvmcpe eveavveklf ilkppdgqlp
      361 vnkgleinsv etagvgylhd ssylpagesg rrlpeigeeg eevggdregg vaeeisthsy
      421 ahtyihthah vhthpqshsp vylpapsset gsgierdsrg qnfsegpqtt sevdkvtpge
      481 vyllakrlsa kkapgpdkis savtkkfamg asrwlahift tcfrrgywpi awktgrlvll
      541 pkgkaqnkge rayrplsiip clakllekvv krriidelak ndladnqygf rrgrstldam
      601 aelrshweaa rkegrhcllv lldvknafnt vrwrsiircm eergfshtli almqsyleer
      661 wivfdskegl lrfqvfggvp qgsvigpilw nlvydglprl rlrrdvyhie yaddigivvi
      721 eedfdrmirv veltiedmsr wyeaeglsla hhkteaillt gctlkrpvak p
//
LOCUS       XP_076549222             171 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611129 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549222
VERSION     XP_076549222.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693107.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="uncharacterized protein LOC117611129 isoform X1"
                     /calculated_mol_wt=19504
     CDS             1..171
                     /gene="LOC117611129"
                     /coded_by="XM_076693107.1:1365..1880"
                     /db_xref="GeneID:117611129"
ORIGIN      
        1 mtriieryhc rpkefdgclc lylyldldhr aadyggclmv tlccasnmaq lpaalsvflp
       61 viriieryhc gpkefdgcly lyldldhraa dyrgglmvtl syasymaqlp aalsiylpmi
      121 riiarhchag lmssmgiwiw iwiwiitlqi vgvmelrkig lrivvlrfpl t
//
LOCUS       XP_076549223             171 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611129 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549223
VERSION     XP_076549223.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693108.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="uncharacterized protein LOC117611129 isoform X1"
                     /calculated_mol_wt=19504
     CDS             1..171
                     /gene="LOC117611129"
                     /coded_by="XM_076693108.1:2103..2618"
                     /db_xref="GeneID:117611129"
ORIGIN      
        1 mtriieryhc rpkefdgclc lylyldldhr aadyggclmv tlccasnmaq lpaalsvflp
       61 viriieryhc gpkefdgcly lyldldhraa dyrgglmvtl syasymaqlp aalsiylpmi
      121 riiarhchag lmssmgiwiw iwiwiitlqi vgvmelrkig lrivvlrfpl t
//
LOCUS       XP_076549224             171 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611129 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549224
VERSION     XP_076549224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693109.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="uncharacterized protein LOC117611129 isoform X1"
                     /calculated_mol_wt=19504
     CDS             1..171
                     /gene="LOC117611129"
                     /coded_by="XM_076693109.1:855..1370"
                     /db_xref="GeneID:117611129"
ORIGIN      
        1 mtriieryhc rpkefdgclc lylyldldhr aadyggclmv tlccasnmaq lpaalsvflp
       61 viriieryhc gpkefdgcly lyldldhraa dyrgglmvtl syasymaqlp aalsiylpmi
      121 riiarhchag lmssmgiwiw iwiwiitlqi vgvmelrkig lrivvlrfpl t
//
LOCUS       XP_076549225             171 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611129 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549225
VERSION     XP_076549225.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693110.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="uncharacterized protein LOC117611129 isoform X1"
                     /calculated_mol_wt=19504
     CDS             1..171
                     /gene="LOC117611129"
                     /coded_by="XM_076693110.1:1365..1880"
                     /db_xref="GeneID:117611129"
ORIGIN      
        1 mtriieryhc rpkefdgclc lylyldldhr aadyggclmv tlccasnmaq lpaalsvflp
       61 viriieryhc gpkefdgcly lyldldhraa dyrgglmvtl syasymaqlp aalsiylpmi
      121 riiarhchag lmssmgiwiw iwiwiitlqi vgvmelrkig lrivvlrfpl t
//
LOCUS       XP_076549226             164 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611129 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549226
VERSION     XP_076549226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693111.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..164
                     /product="uncharacterized protein LOC117611129 isoform X2"
                     /calculated_mol_wt=18207
     CDS             1..164
                     /gene="LOC117611129"
                     /coded_by="XM_076693111.1:1415..1909"
                     /db_xref="GeneID:117611129"
ORIGIN      
        1 msvsvsvsgs gssccrlrrl rrvgcahcgc lmvtlccasn maqlpaalsv flpviriier
       61 yhcgpkefdg clylyldldh raadyrgglm vtlsyasyma qlpaalsiyl pmiriiarhc
      121 haglmssmgi wiwiwiwiit lqivgvmelr kiglrivvlr fplt
//
LOCUS       XP_076549227             105 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611129 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549227
VERSION     XP_076549227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693112.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="uncharacterized protein LOC117611129 isoform X3"
                     /calculated_mol_wt=11917
     CDS             1..105
                     /gene="LOC117611129"
                     /coded_by="XM_076693112.1:1365..1682"
                     /db_xref="GeneID:117611129"
ORIGIN      
        1 mtriieryhc rpkefdgclc lylyldldhr aadyggclmv tlccasnmaq lpaalsvflp
       61 viriieryhc gpkefdgcly lyldldhraa dyrgyvetgv rtmva
//
LOCUS       XP_076549228             856 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306827 [Osmia lignaria lignaria].
ACCESSION   XP_076549228
VERSION     XP_076549228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693113.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..856
                     /product="uncharacterized protein LOC143306827"
                     /calculated_mol_wt=95966
     Region          6..204
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(10,12,37,93,126,128,202)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(10,37,93,126,128,176,201..202)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(37,201)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          <404..>479
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          553..809
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(642..647,701..702,731,733..734,805..806)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(642..647,701,733)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            702
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     CDS             1..856
                     /gene="LOC143306827"
                     /coded_by="XM_076693113.1:288..2858"
                     /db_xref="GeneID:143306827"
ORIGIN      
        1 msaftvlqan lnhcraaqdi lwqaahdyra divvisephr prpewhtdps gtlsmwcvqr
       61 tpptaelgmi rkihtsdgci gletrdfvif gcyfspsltm atyegqldgl aagvasstkp
      121 viisgdfnar svqwgsdrtd argyrllrvc eqsglipvns kggpsfvrnh qqsridavac
      181 shalrsrlks sfvstkytgs dhnylvhrfs atdtfesssm nsmwntsynp pdkeawasii
      241 knnlslppet dawvrelpih vfedtasidt aiaeleswye sariprshns arkrkyvaww
      301 tkeisrlrln aarsrakwrr aalnmsknnr nvsvdsqhpl rqlmvsdrra likairqskd
      361 aswsgmidei arypwgkpyk avmnrlkgrs pllrlnetef ktavsqlfvt eppvttagsa
      421 pvpdvtnptp aprnrsaspq pgpscttpsr stsigpaapp svnrpitnst sptpsftepi
      481 crvapedpri pypfstrdav aalakvsggk apgrdgitge alrpimrlap ewirdlfnac
      541 yrlgyfpnsw kvgrlvlipk pgkpfssysd lrplcmlsnl gkgfeyalre lleeavqnsg
      601 dlspwqfgfr kkrstmqamk vvisewnrar sygnhcllig ldvknafntv rweniilaaq
      661 rrhfpphlia mlqnylnsrf igttmpdgtw ieqevfagvp qgsvlgpflw nlaydsilev
      721 vlprnamtia faddlaliil aalsqireia eealrrlsvw ylqngltlat hkteailltg
      781 rripaglilq hetsvirtit smrylgvlfn nnrtfrshiv knktdrhrnn sktqqynvdv
      841 tkwhritlvn mqviin
//
LOCUS       XP_076549229             300 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306828 [Osmia lignaria lignaria].
ACCESSION   XP_076549229
VERSION     XP_076549229.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693114.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478179) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="uncharacterized protein LOC143306828"
                     /calculated_mol_wt=34627
     Region          <1..126
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(15,51,124)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Region          <230..>298
                     /region_name="PRK00409"
                     /note="recombination and DNA strand exchange inhibitor
                     protein; Reviewed"
                     /db_xref="CDD:234750"
     CDS             1..300
                     /gene="LOC143306828"
                     /coded_by="XM_076693114.1:1..903"
                     /db_xref="GeneID:143306828"
ORIGIN      
        1 mvainynnfl iiseyispni dvvtvderld eiseviksnk wsgillagdf nskspvwggn
       61 twnargkill eyvlseslsp iytvggntci kgngskidll itstnlcykv ttsevldeft
      121 gsdhlylwhe vnlenpeidk tksirnknkk tknrgnkrmn rstidsnkyv nkflekhgdr
      181 ennrysinqt vndikkvled mqkigsnctk rnplrpgrcp vhwwtpeiae qrkatnklrr
      241 kvtrlrrkke kelselaere fklakkelnk kirnsrketv ekliktidnd mwgealqndn
//
LOCUS       XP_076549230             530 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306877 [Osmia lignaria lignaria].
ACCESSION   XP_076549230
VERSION     XP_076549230.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693115.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..530
                     /product="uncharacterized protein LOC143306877"
                     /calculated_mol_wt=58188
     Region          15..90
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          117..145
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cd09917"
                     /db_xref="CDD:438852"
     Site            order(117..118,120..122,125..126,129,140..142,144)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(117,121,124..125,128..129,133,135..136,140..142,144)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          190..206
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          191..>357
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          219..243
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          244..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          292..317
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          318..347
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          348..427
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <393..>511
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          428..476
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          477..502
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..530
                     /gene="LOC143306877"
                     /coded_by="XM_076693115.1:1714..3306"
                     /db_xref="GeneID:143306877"
ORIGIN      
        1 masryrntke enegrivfvt rippftktke llklfkefrp ehavvarkst gtqgyvtfpq
       61 atlaaeaiaw vkntqpifhk rriraaladp wheakllrpr dgavpfpgwe prpspplpie
      121 aitriarllp fadrarmecv srawrmgsma syagtnrldl tdwrwpqawe srrittemln
      181 wavrrlgvyi tdlrlddgrv vgrlrpqvlg ivvrhcpnlv kidltgtllr psavrdlias
      241 anslrelelg sclgsvdpel salfaaatrl erfgatgtaf vgaslprltp alrslrvkrc
      301 eslspapvaa flraarslvd lelaqctait teeplhalia ntalhstlee lsicdinfvp
      361 apepaadddd fevppdqplh flvlqigpae aatsipeafk nlknlstmfc gwvgngfvih
      421 igehlaqltd lnlsgcsnir gqfalepler ltalrrlain nlhptvgaqv lgnistleev
      481 ncrdslgvtd edactaaqgc pnlvalnmeg clgitertlr gitnivrgqg
//
LOCUS       XP_076549232             282 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306879 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549232
VERSION     XP_076549232.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693117.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="uncharacterized protein LOC143306879 isoform X1"
                     /calculated_mol_wt=32454
     Region          29..92
                     /region_name="THAP"
                     /note="THAP domain; cl02739"
                     /db_xref="CDD:470662"
     CDS             1..282
                     /gene="LOC143306879"
                     /coded_by="XM_076693117.1:104..952"
                     /db_xref="GeneID:143306879"
ORIGIN      
        1 mqqqqvkmem kkrmkvcsvr sctqqytrmf tfpqekkdkk rmmqwveacd hpnllilppe
       61 klkfwvicss hieekyfmrk rlttsavptl yllvseaasi kikqqetlme ipeeeeeckp
      121 dctggmdtkc givghvdfta lpgpsgemqc ndlgrlslqe dkplhnadcv vlgrehqrse
      181 aqkghkgnil kmlkdnivek plqqriysav aavwkaarqc krrqmatkdr ikkiehlvqv
      241 gvcqvlenlp trddfrldaq rgkthlwttl hgrgkgisfk yl
//
LOCUS       XP_076549233             187 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306879 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549233
VERSION     XP_076549233.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693118.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="uncharacterized protein LOC143306879 isoform X2"
                     /calculated_mol_wt=21460
     Region          29..92
                     /region_name="THAP"
                     /note="THAP domain; cl02739"
                     /db_xref="CDD:470662"
     CDS             1..187
                     /gene="LOC143306879"
                     /coded_by="XM_076693118.1:104..667"
                     /db_xref="GeneID:143306879"
ORIGIN      
        1 mqqqqvkmem kkrmkvcsvr sctqqytrmf tfpqekkdkk rmmqwveacd hpnllilppe
       61 klkfwvicss hieekyfmrk rlttsavptl yllvseaasi kikqqetlme ipeeeeeckp
      121 dctggmdtkc givghvdfta lpgpsgemqc ndlgrlslqe dkplhnadcv vlgrehqrse
      181 aqkghkd
//
LOCUS       XP_076549234             149 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306880 [Osmia lignaria lignaria].
ACCESSION   XP_076549234
VERSION     XP_076549234.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693119.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="uncharacterized protein LOC143306880"
                     /calculated_mol_wt=17326
     CDS             1..149
                     /gene="LOC143306880"
                     /coded_by="XM_076693119.1:299..748"
                     /db_xref="GeneID:143306880"
ORIGIN      
        1 mdgiqaikkm sipttatnnr ysvlqyliei dqdenntaaq qtpvtqrnnq ftkklsplvm
       61 hdnnisdfen lkktwkekgs nfhtytrkde kkrvyvingl hnkitaeetk eelyrheina
      121 dnitkmkrtv rslymisvsn aiklkhlrt
//
LOCUS       XP_076549235             282 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306881 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549235
VERSION     XP_076549235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693120.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="uncharacterized protein LOC143306881 isoform X1"
                     /calculated_mol_wt=32454
     Region          29..92
                     /region_name="THAP"
                     /note="THAP domain; cl02739"
                     /db_xref="CDD:470662"
     CDS             1..282
                     /gene="LOC143306881"
                     /coded_by="XM_076693120.1:104..952"
                     /db_xref="GeneID:143306881"
ORIGIN      
        1 mqqqqvkmem kkrmkvcsvr sctqqytrmf tfpqekkdkk rmmqwveacd hpnllilppe
       61 klkfwvicss hieekyfmrk rlttsavptl yllvseaasi kikqqetlme ipeeeeeckp
      121 dctggmdtkc givghvdfta lpgpsgemqc ndlgrlslqe dkplhnadcv vlgrehqrse
      181 aqkghkgnil kmlkdnivek plqqriysav aavwkaarqc krrqmatkdr ikkiehlvqv
      241 gvcqvlenlp trddfrldaq rgkthlwttl hgrgkgisfk yl
//
LOCUS       XP_076549236             187 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306881 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076549236
VERSION     XP_076549236.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693121.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="uncharacterized protein LOC143306881 isoform X2"
                     /calculated_mol_wt=21460
     Region          29..92
                     /region_name="THAP"
                     /note="THAP domain; cl02739"
                     /db_xref="CDD:470662"
     CDS             1..187
                     /gene="LOC143306881"
                     /coded_by="XM_076693121.1:104..667"
                     /db_xref="GeneID:143306881"
ORIGIN      
        1 mqqqqvkmem kkrmkvcsvr sctqqytrmf tfpqekkdkk rmmqwveacd hpnllilppe
       61 klkfwvicss hieekyfmrk rlttsavptl yllvseaasi kikqqetlme ipeeeeeckp
      121 dctggmdtkc givghvdfta lpgpsgemqc ndlgrlslqe dkplhnadcv vlgrehqrse
      181 aqkghkd
//
LOCUS       XP_076549237             119 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306882 [Osmia lignaria lignaria].
ACCESSION   XP_076549237
VERSION     XP_076549237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693122.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..119
                     /product="uncharacterized protein LOC143306882"
                     /calculated_mol_wt=12904
     CDS             1..119
                     /gene="LOC143306882"
                     /coded_by="XM_076693122.1:75..434"
                     /db_xref="GeneID:143306882"
ORIGIN      
        1 msdhemevvn tdenkedien iqekkeeekq kedgkngqed gknrqeekek plssskikkl
       61 lkkmvygggg grrgkwggrr ggrgwrgggg rgrgggrgwg ggrgrgsgtg grnvivnyy
//
LOCUS       XP_076549238             245 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306883 [Osmia lignaria lignaria].
ACCESSION   XP_076549238
VERSION     XP_076549238.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693123.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..245
                     /product="uncharacterized protein LOC143306883"
                     /calculated_mol_wt=24939
     CDS             1..245
                     /gene="LOC143306883"
                     /coded_by="XM_076693123.1:553..1290"
                     /db_xref="GeneID:143306883"
ORIGIN      
        1 mfsngriscs dffgaqqnmd nsayvpnhlp ppslvvfsqa gglpealrmq rpfnpqvtds
       61 kdlqvpfsta aslgyglhhm lhlppqflhp ldhrlpfggf rplgpsafap pskclkvetg
      121 ngpvsgsglp sisslssmsn mfspsslssa sgggvvsvsg asasiggvgg gagntaggqe
      181 vapqspagst srsptgtvtg tgtgslpnrg atpededrdt natpgsente rstpeegrpy
      241 rrlsf
//
LOCUS       XP_076549239             277 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306884 [Osmia lignaria lignaria].
ACCESSION   XP_076549239
VERSION     XP_076549239.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693124.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..277
                     /product="uncharacterized protein LOC143306884"
                     /calculated_mol_wt=30781
     CDS             1..277
                     /gene="LOC143306884"
                     /coded_by="XM_076693124.1:1..834"
                     /db_xref="GeneID:143306884"
ORIGIN      
        1 mrelsrrpps ssshssrrge tpervdrdrp nqneetgnqs kaqtnienit adkptdkpie
       61 entpsgssee vpkqvpehei eleeeiinvl gvrletdkkp aaavhkdval rwseilrkgl
      121 pteevkklve kypapencsf ivvpklkaev naaiqenaik rdkrivekqe riaaclaavg
      181 kaiaitlkid dpkkvelfek lsdggrllas ihreeslark glilanlnss fkatlsntsi
      241 dewlfgvdld eklktaknle kaskelkplt kpihttn
//
LOCUS       XP_076549240             555 aa            linear   INV 03-SEP-2025
DEFINITION  pyroglutamylated RF-amide peptide receptor-like [Osmia lignaria
            lignaria].
ACCESSION   XP_076549240
VERSION     XP_076549240.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693125.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478180) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..555
                     /product="pyroglutamylated RF-amide peptide receptor-like"
                     /calculated_mol_wt=62870
     Region          32..337
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          32..54
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          64..86
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          102..124
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          147..163
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          185..208
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          267..289
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          305..330
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     Region          437..527
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(451,453,455,481..483,521)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            451..453
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            451
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(481..484,488..491)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     CDS             1..555
                     /gene="LOC143306885"
                     /coded_by="XM_076693125.1:538..2205"
                     /db_xref="GeneID:143306885"
ORIGIN      
        1 msnttndfld yninestfsq fdwtelaftl gfsltfslgl ignvfiiist lctklrplpa
       61 tppnvflggl atadlvliif cilikvaklf sytwtlgafl cksvnyiqrv svicsavmlt
      121 amsieryfai iypmkteyrf tisqarkvvi tiwvtsfflg ipmifvqthk vywcvrdsda
      181 allwrghevy mlvlilalpl tvmvfcywki rlvmkgryhm tarhvlnsnn adtesismte
      241 mqnmrndhyy redgrteeef rtvkqvmkml vamgvvfavc wgpvlidntl taysvlpqvk
      301 qgmyihfnta fqlmafynsc vnpiiyafis nqfrksflsa fcgglkgghr apmerhpsls
      361 ercrdvemwp sqspkdgmpv prtsplqlpr nvrcedrrlp lpsggkptpd rtrrrnpaev
      421 rpdtcqrevn engcihlefa vhgqryhalv dtgatrsllr sdlaelcrrv gdpwspsqwv
      481 vrvangaeav tdgeievlat idgqpirhry gvlptlsqti llgmdvlgqm gtsmrigqhq
      541 wdcqteafep agpte
//
LOCUS       XP_076549241             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306887 [Osmia lignaria lignaria].
ACCESSION   XP_076549241
VERSION     XP_076549241.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693126.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478181) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC143306887"
                     /calculated_mol_wt=54677
     Region          <117..239
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     Region          402..>464
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..484
                     /gene="LOC143306887"
                     /coded_by="XM_076693126.1:940..2394"
                     /db_xref="GeneID:143306887"
ORIGIN      
        1 mddsdetptv pkgtkrrals spslspaeia dkavssaatr eklpieiadf eefdeevrgh
       61 lynitvsqps keskartqav eeainclhhf ykkvtrayie vaakwtelst lksvflkakd
      121 ednslhnitd inkniddklv kmkdniindi katvqkelye tvslsienvv ksndmmkeni
      181 qsniknvvsn evkeavhevq kvvkdsiqsa kpsyasitnk sapylknrei tprnkqsief
      241 lisplpekvk nfksasevkr vlknsvnpve ydlkvdhlft vgmssvkiia nsvdldklrn
      301 spklkeaglm irtrqklrpr lliknlpsni smndvpkvia esigienpei kpivelirge
      361 kkqknliiev speirkklls hgrvylgyds criedyisvl qcykclkfnh takncttdsp
      421 tcghcadkhd trkcenktkp kcvncdklgy ddvihsaldf rkcpvilkrm eeltrsidyg
      481 ddqp
//
LOCUS       XP_076549250             206 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143307777 [Osmia lignaria lignaria].
ACCESSION   XP_076549250
VERSION     XP_076549250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693135.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478218) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..206
                     /product="uncharacterized protein LOC143307777"
                     /calculated_mol_wt=23123
     CDS             1..206
                     /gene="LOC143307777"
                     /coded_by="XM_076693135.1:68..688"
                     /db_xref="GeneID:143307777"
ORIGIN      
        1 mtkhqndvki atqlscrilg rkypltdsct kyleiginil gdnfypqlvl sdsrnnrlel
       61 sivtwsliva eadnvcsffd dskdtrdsin fnnisvefgv lygekvikli sndmcmcmmk
      121 ktfekmviln dcivemhnal ydnlysveqk fkefvalvrg ygdvkvpaev vrhiketdiy
      181 dpnsiidcel valgiqaiit aasaas
//
LOCUS       XP_076549252             461 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143307813 [Osmia lignaria lignaria].
ACCESSION   XP_076549252
VERSION     XP_076549252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693137.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478223) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 52% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..461
                     /product="uncharacterized protein LOC143307813"
                     /calculated_mol_wt=52067
     Region          143..287
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          <385..>439
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..461
                     /gene="LOC143307813"
                     /coded_by="XM_076693137.1:1..1386"
                     /db_xref="GeneID:143307813"
ORIGIN      
        1 mpnnsasasq tqvatrgsni gtlrrkrghi igqisslttf ldyfedsdsr dtfllqthld
       61 glnetwkkfd diqfdieeld qseetrryei qnnyyaaiar anrllhneqr ttpaacntvs
      121 sprpsttsap maiklpemrl ptfngtiegw asfhdiftsm idrnedltpv qkfqylrstl
      181 tgkaaaciqa lsttdanyhd aiellrekfd cprrailghc dalrdipklv kdtpealgdl
      241 vdtvnqhlra ltnlgerves wnslllsmim sklnsntvwh welalkdkrt vpaytdllaf
      301 lkkrancaqi ssakvtptae hrgagpsgnp riqrhrpsrg haflsaktrh hpyedrrtre
      361 kqhisapprr cpvcdgnhii wfcdrfhgss vrdriavvkr tslcqnclrd ghnadacthg
      421 tcricrkkhh tllhqtatie eptsrraaes sqstettrqp i
//
LOCUS       XP_076549253             653 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143307814 [Osmia lignaria lignaria].
ACCESSION   XP_076549253
VERSION     XP_076549253.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693138.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478223) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..653
                     /product="uncharacterized protein LOC143307814"
                     /calculated_mol_wt=73221
     Region          231..445
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(295..300,334..335,370,372..373,436..437)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(295..300,334,372)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            335
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          461..613
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..653
                     /gene="LOC143307814"
                     /coded_by="XM_076693138.1:1..1962"
                     /db_xref="GeneID:143307814"
ORIGIN      
        1 msrhtvtati qsktntynrt ltfltiptis aflpdqpidr apinipkniq ladptfhipa
       61 pvdlllgagv alsllsvgqi nlsppngpdl flqktrlgwi iggsapaskp arspichatt
      121 tiqddlarfw eleegpqiqr lsdaericee hfqrtvrrnp ggryvvalpf ndklpqlges
      181 ksralkhlla lerrlqhdqg lsqqyrsvlq eyqdlghmse irdtdeisdg yylphhgvtk
      241 tssdttklrv vfdgsaattt gvslnetlhv gpkvqdnlly ilirfrshry vlngdiekmy
      301 rqflvrpedr kyqrilwrdp agnlktyaln tvtfglsaap ylairclsql aqdeghrfpd
      361 aaqnlvrdfy vddlltgast vkgalalrdq miqllnsagl qirqwasnhk pllhglpeqd
      421 inkrhhlges stiktlgvvw dstddtiqys vtnvtptksv tkrsissvia riydplgllg
      481 pviivakill qkiwtmkidw deslpmtvht ewmqyckqlp llnnitfhrn tlineptsve
      541 lhgfcdaser aygaciylrs rnadghyqve lllakskvap lktqsiprle lcgavlltsl
      601 attaqralhi iidlapwtel vtrrgntlaf rkhtmskrss gtenrrllnh hss
//
LOCUS       XP_034194686             442 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610940 [Osmia lignaria lignaria].
ACCESSION   XP_034194686
VERSION     XP_034194686.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338795.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478223) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194686.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..442
                     /product="uncharacterized protein LOC117610940"
                     /calculated_mol_wt=50353
     Region          58..108
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          153..221
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          337..428
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..442
                     /gene="LOC117610940"
                     /coded_by="XM_034338795.2:1..1329"
                     /db_xref="GeneID:117610940"
ORIGIN      
        1 mkrlktgndq alkgrlqrln pfidrdgllr vggrlknsfm pfsqrhpiil pktrvtalil
       61 gnehraqlha gtqatlyavr rrywpidgrn qvwkvvkacv rccraqpppv dyimgnlpaa
      121 rvtesrpftn vgvdycgpff ikerkhrnls rvkvyvavfv clavkavhle lvseltteaf
      181 laalrrfiar rgfctdlysd ngsnfvgann elrelrsllq sddhrkkvtt flaerainwn
      241 fippltphfg glweaavksf kyhlkrvagt elftfenfnt liieiesiln srpltpissd
      301 pndllvltpa hfligdslts lrernfrdtp snrlsswqhv qkvkqhfwtr whreylnelt
      361 trskwttggh aigegtivll rednipslhw plgrvikvhp gsdgiiraat iktakgtldr
      421 svkrlvplpv qtdqdeqimd sh
//
LOCUS       XP_076549254             501 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143307946, partial [Osmia lignaria
            lignaria].
ACCESSION   XP_076549254
VERSION     XP_076549254.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693139.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478248) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..>501
                     /product="uncharacterized protein LOC143307946"
     Region          14..228
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(78..83,117..118,153,155..156,219..220)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(78..83,117,155)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            118
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          244..404
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..501
                     /gene="LOC143307946"
                     /coded_by="XM_076693139.1:1..>1503"
                     /db_xref="GeneID:143307946"
ORIGIN      
        1 mtkvtniqcp sdgyylphhg vtkitsettk lrvvfdgsaa tssgislnda lhigpriqed
       61 llyillrfrv hryvitgdie kmyrqflvrd edqkyqrvlw rdasreiqty elrmvtfgls
      121 aapylaircl tqlaqdechr fpraaqilrr dfyvddlltg astlqeaasl redltqllkl
      181 aslnirqwas nhedllrnlp eesinrkiri gesstlktlg iiwdsandsi syevktqian
      241 syvtkrfits eiakiydplg ligpviivak mllqriwtik vdwdeslpmd ihtewkqfhr
      301 qlpllnstvf rrrtvidtps rtelhgfcda sekaygaciy irssdmhdnt tvelllaksk
      361 vaplktqsip rlelcgalll ttlyntvkka lhvklnrtif wtdstvvlhw lqtsphtlkt
      421 fvanriseiq artnigdwrh vpttdnpadl isrgqhpeef lqpsiwhhgp kwlkheevsw
      481 ptrdlvpcnf ipeqrtatcl k
//
LOCUS       XP_076549255             395 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143307956 [Osmia lignaria lignaria].
ACCESSION   XP_076549255
VERSION     XP_076549255.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693140.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478251) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 81% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..395
                     /product="uncharacterized protein LOC143307956"
                     /calculated_mol_wt=44746
     Region          77..221
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          <319..>373
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..395
                     /gene="LOC143307956"
                     /coded_by="XM_076693140.1:1..1188"
                     /db_xref="GeneID:143307956"
ORIGIN      
        1 mkfddiqfdi eeldqseetr ryeiqnnyya aiarvnrllh neqrttpaac ntvssprpst
       61 tsapmaiklp emrlptfngt iegwasfhdi ftsmidrned ltpvqkfqyl rstltgkaaa
      121 ciqalsttda nyhdaiellr ekfdcprrai lghcdalrdi pklvkdtpea lgdlvdtvnq
      181 hlraltnlge rveswnslll smimsklnsn tvwhwelalk dkrtvpaytd llaflekran
      241 caqissakvt ptaehrgagp sgnpriqrhr psrghaflsa ktrhhpyedr rtrekqhisa
      301 pprrcpvcdg nhiiwfcdrf hgssvrdria vvkrtslcqn clrdghnada cthgtcricr
      361 kkhhtllhqt atieeptsrr aaessqstet trqpi
//
LOCUS       XP_076549256             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143307957 [Osmia lignaria lignaria].
ACCESSION   XP_076549256
VERSION     XP_076549256.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693141.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478251) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC143307957"
                     /calculated_mol_wt=54186
     Region          214..428
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(278..283,317..318,353,355..356,419..420)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(278..283,317,355)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            318
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     CDS             1..484
                     /gene="LOC143307957"
                     /coded_by="XM_076693141.1:1..1455"
                     /db_xref="GeneID:143307957"
ORIGIN      
        1 msrhtvtati qsktntynrt ltfltiptis aflpdqpidr apinipkniq rrgslsllsv
       61 gqinlsppng pdlflqktrl gwiiggsapa skparspich atttiqddla rfweleegpq
      121 iqrlsdaeri ceehfqrtvr rnpggryvva lpfndklpql ggsksralkr llalerrlqh
      181 dqglsqqyrs vlqeyqdlgh mseirdtdei sdgyylphhg vtktssdttk lrvvfdgsaa
      241 tttgvslnet lhvgpkvqdn llyilirfrs hryvlngdie kmyrqflvrp edrkyqrilw
      301 rdpagnlkty alntvtfgls aapylaircl sqlaqdeghr fpdaaqnlvr dfyvddlltg
      361 astvkgalal rdqmiqllns aglqirqwas nhkpllhglp eqdinkrhhl gesstiktlg
      421 vvwdstddti qysvtnvtpt ksvtkrsiss viariaiivn qqpsgllidg nkhtaiklkp
      481 vqyi
//
LOCUS       XP_076549257            1209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612080 [Osmia lignaria lignaria].
ACCESSION   XP_076549257
VERSION     XP_076549257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693142.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478253) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1209
                     /product="uncharacterized protein LOC117612080"
                     /calculated_mol_wt=138478
     Region          <2..77
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          207..421
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(271..276,310..311,346,348..349,412..413)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(271..276,310,348)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            311
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          437..597
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          814..864
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          909..1003
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1093..1184
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1209
                     /gene="LOC117612080"
                     /coded_by="XM_076693142.1:1316..4945"
                     /db_xref="GeneID:117612080"
ORIGIN      
        1 mipkiaslip dqqidrsnik ipknlnladp efhrpapidl llgsgtalsl vsvgqinlsp
       61 pdgpdlylqk trlgwviggs ppkpiksriv scnattalqh dlarfweiee gpqlqllses
      121 dkfcekhfqq hvkrnsdgry vvalpfndkl lllgesrara lkrleslerk lqrdatlrge
      181 yhaviqeyld lghmtkvtni qcpsdgyylp hhgvtkitse ttklrvvfdg saatssgisl
      241 ndalhigpri qedllyillr frvhryvitg diekmyrqfl vrdedqkyqr vlwrdasrei
      301 qtyelrtvtf glsaapylai rcltqlaqde chrfpraaqi lrrdfyvddl ltgastlqea
      361 aslredltql lklaslnirq wasnhedllr nlpeesinrk irigesstlk tlgiiwdsan
      421 dsisyevktq iansyvtkrf itseiakiyd plgligpvii vakmllqriw tikvdwdesl
      481 pmdihtewkq fhrqlpllns tvfrrrtvid tpsrielhgf cdasekayga ciyirssdmh
      541 dnttvellla kskvaplktq siprlelcga lllttlyntv kkalhvklnr tifwtdstvv
      601 lhwlqtspht lktfvanris eiqartnigd wrhvpttdnp adlisrgqhp eeflqpsiwh
      661 hgpewlkhee vtwptrdlvp cnfipeqrta tclkvspldt nilddysswe kmqrivawcl
      721 rwkgdnmikg pltpvelrha rdviikllqr ihfskelrcl ssssnpeiag klqrlspfvd
      781 kdgivrvggr lkhssmpftq rhpiilpkar vtgliieaeh raqlhagvqg tlyavrrrfw
      841 pidgrnqvwk alrtctrclr arppatnyim gelpearvte arpftnvgvd ycgpfyiker
      901 rhrnrtrikv yvavfvclav kavhlelvsd ltseafiaal rrfiarrgyc vkihsdngtn
      961 fvganneiqe iqrlirsdeh ntkvqnflaq rsiewsfipp raphfgglwe aavksfknhl
     1021 lrvagtelft fedfntliie iesilnsrpl tpmssdpndl lvltpghfli gdsltslrer
     1081 dyldtppnrl sswqfiqrik qhfwtrwhre ylneltrrtk wskgehpike gtlvllredn
     1141 vppmqwalgr vhrtypgsdg iirtvtikta aseldrsvkr lvplpyqvee rsnepeepas
     1201 tanvnpidd
//
LOCUS       XP_076549259             767 aa            linear   INV 03-SEP-2025
DEFINITION  U3 small nucleolar RNA-associated protein 14 homolog A-like [Osmia
            lignaria lignaria].
ACCESSION   XP_076549259
VERSION     XP_076549259.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693144.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..767
                     /product="U3 small nucleolar RNA-associated protein 14
                     homolog A-like"
                     /calculated_mol_wt=89100
     Region          19..757
                     /region_name="Utp14"
                     /note="Utp14 protein; pfam04615"
                     /db_xref="CDD:461368"
     CDS             1..767
                     /gene="LOC143308076"
                     /coded_by="XM_076693144.1:192..2495"
                     /db_xref="GeneID:143308076"
ORIGIN      
        1 msdleflddt eqdvskfhsk lldavsqldk gqrvkkaers eptlevsefh lvksgvsdld
       61 tvrvhdlakv lgkkghhldv tkslesakrk irvlqkplek paaerikriv gfenakkelk
      121 kwnaiitknr taetlhfpln qpsmklepsk efvkrfriqs dlekelalle pqkenieqke
      181 dtlkltlnev imrrkeaari raqqsykear arrqnkiksk kfhrvqkkek iklqlkefeq
      241 lqktnpeaal ekleqldktr aeermslrhk ntgkwakskl imakydketr qelaqqlsis
      301 reltqklkks tdreeddeee desinytayl rssndkenpw inntksesei dqfissyrky
      361 wddqksqfkn qqsdtinknk ieienkqtei ssknlkdsni snitlhyntv sktespkiqh
      421 vqeipkqksn nqitpldhlk tstfqdqnst cnvesfkkqn hkknekilaf naetikhsss
      481 nitikrklsn nnkkmdkkan lhasnnitat sawnveflen nhapnvsnsl tsghsnteki
      541 nkmfdvmedk mndqielklq qvtqkyitak rkskfkniie dekdhfdgle iqaknrrpii
      601 dssliesitn gsleldtelq nsknikydnv lsvnksntse veidpnkyin vkpkqlktdi
      661 padilegnda lddvedeeer ynviseafad ddvveefrke keqeveqsrp kdidltlpgw
      721 gnwggknvka srrkrkrlii kvpkdiprkd ekppniieiq khrgpfl
//
LOCUS       XP_076549260            1339 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143308077, partial [Osmia lignaria
            lignaria].
ACCESSION   XP_076549260
VERSION     XP_076549260.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693145.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..1339
                     /product="uncharacterized protein LOC143308077"
     Region          36..107
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); smart00868"
                     /db_xref="CDD:214871"
     Region          714..734
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          743..764
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <757..>941
                     /region_name="COG5236"
                     /note="Uncharacterized conserved protein, contains RING
                     Zn-finger [General function prediction only]"
                     /db_xref="CDD:227561"
     Region          773..794
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          802..820
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          832..851
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          857..878
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          858..878
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(863,865,867,869..870,873..874,877,891,893,897..898,
                     901..902,905,924,926,928,930..931,934..935,939)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          886..906
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          919..940
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <936..1013
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          948..1013
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          992..>1044
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          992..1013
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Site            order(997,1003,1010,1012,1020,1027)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          1021..1042
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          1021..1042
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1051..1071
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1079..1100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1084,1086,1088,1090..1091,1094..1095,1099,1113,1115,
                     1119..1120,1123..1124,1127,1141,1143,1145,1147..1148,
                     1151..1152,1155)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1108..1128
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1136..1156
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1339
                     /gene="LOC143308077"
                     /coded_by="XM_076693145.1:<1..4020"
                     /db_xref="GeneID:143308077"
ORIGIN      
        1 danslneltk islhnkisaq kklsileqtk qelkkycrtc aglkfplvdi fsekgiqmrl
       61 sqqikhleei nsydslstqm cmdcicdlkm sykffmqikk aevklksihf tltetatrnl
      121 eakkmqsver iekhvenive dnflnpstsk ehneevlvqk vpedipvkdn lhcikigkvn
      181 veelekpeve vfeemiieen sdnesieefd pddppfqpds sdaaesddev vvqtpavkrr
      241 niqkeniprq yppsmflcdy nsapdnestd cnqttykyds slkayikied tegnnhscep
      301 qniirtetac naestkestk qpnilkrrll lqtrkdlsee tdenqsgvsd kngghkdfkv
      361 qkldvnnsln instqedgim yvtvkgskpn eillvkvkkm dkplekkddk clgkkpfeik
      421 pmdkifkrye tlvtkekdlf pertkkseir eqiieeqiee ykkkrekvlg ghasislpik
      481 teepqtryfk rnesqdsqkp tphaenestv nvqfikdedd ctpkiendit dtaaepekek
      541 svneksyvnt eeylsrlakl klkweeakkk keslqkelde sytegniekl arilgekekn
      601 lqdfqdylkq rkivitrlkd ediislyedr nnavlknnlp dlidesqpad yipeenylec
      661 dycpetfask dileehlkmh dykimhfced cmeefptnka krnhsvvcik kllckycevm
      721 ldskgkkrqh eqkhcdnmyg qlcdvcgekf khqgtldqhv ktqhmsweki yqcpkcpkkf
      781 afkqklsfhl ksvhttlray lcedcgadfk npaslrhhri rkhqpvgnkr ecpvchklvp
      841 fyslskhmht hkaytiqcph cdkmfknsst lkqhvrihed qrqyrcdtcg vgfnrrdglr
      901 lhmrvhektd srglkecscq vcgekfpnhs tlvihrnrvh kdgrqytchi cnrsmistrs
      961 lewhmshihn eslpgvvked tdgapekkrv scyhcnktfk temilrthik nthmekdpmk
     1021 cldceltfts evrlrhhmmv thnrlegtlp cphcpkrfvn qlrlkthmis hseerpytce
     1081 icgfnlktki qlikhhqnrh sderplqcry cpwrckqvsa lvcherthtn erpyscsvcr
     1141 qrfkylgdkn kherrheslg gsgfkrivpg rnikpkvqed sscseheqes ltnaehqive
     1201 geyeeqtdqq yeeeqivkfe eqeivkfeeq eiteeyeqvy eqeyeetsre asdaaevimn
     1261 medttvytee vtadniepae iitdemmtne ilqsgavvhl qqeddsgkiq vipvmlslpd
     1321 lsdantevnl atasimynn
//
LOCUS       XP_034189959             629 aa            linear   INV 03-SEP-2025
DEFINITION  bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase-like
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189959
VERSION     XP_034189959.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334068.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189959.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="bifunctional 3'-phosphoadenosine
                     5'-phosphosulfate synthase-like isoform X1"
                     /calculated_mol_wt=70724
     Region          31..225
                     /region_name="CysC"
                     /note="Adenylylsulfate kinase or related kinase [Inorganic
                     ion transport and metabolism]; COG0529"
                     /db_xref="CDD:440295"
     Region          254..623
                     /region_name="ATPS"
                     /note="ATP-sulfurylase; cd00517"
                     /db_xref="CDD:173895"
     Site            order(419..423,429,432,497,522..524,526..527,567..569)
                     /site_type="active"
                     /db_xref="CDD:173895"
     Site            426..429
                     /site_type="other"
                     /note="HXXH motif"
                     /db_xref="CDD:173895"
     Site            458..468
                     /site_type="other"
                     /note="flexible loop"
                     /db_xref="CDD:173895"
     CDS             1..629
                     /gene="LOC117608639"
                     /coded_by="XM_034334068.2:135..2024"
                     /db_xref="GeneID:117608639"
ORIGIN      
        1 mttfrkfiha iikfesvflc ltcsnvseqa hhvsrakrgq aigsvrgfrg ctvwltglsg
       61 agktsiafqv eailvnhgia aygldgdnvr sglncnlgfs kedrkenirr vaevaklfad
      121 sgqiclcsfv spfeedrqma rqihkmsdlp ffeifvdtpl nvceardtkg lykkarqgai
      181 kgftgidqmy erpvnpdlvv ttenctpees aatvidllek qciipvlqkp tipirelfvp
      241 esrissgkae attlqsleis eidvqwlqvl aegwaapltg fmredqylqt qhlkclldgd
      301 kevnqsvpiv laihtedker ldglsaftlk yknkalailr rpefyfhrke ercgwqfgtn
      361 nlghpyvkmi hdsgdwlvgg dievfkrirw hdgldkyrlt pneirtkckk mkadavfafq
      421 lrnpihngha llmqdtkkhl leergfknpv lllhplggwt kdddvplhtr ilqheavlse
      481 gvldsnstll aifpspmmya gpvevqwhak armnaganfy ivgrdpagip hpnktvtldg
      541 nlydpthgar vlsiarglqd leiipfkvaa ydkrhkkmsf fdterkgdfe fisgtkmrtl
      601 akvgenppsg fmspkawsvl aqyyqnlek
//
LOCUS       XP_034189960             623 aa            linear   INV 03-SEP-2025
DEFINITION  bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase-like
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034189960
VERSION     XP_034189960.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334069.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189960.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..623
                     /product="bifunctional 3'-phosphoadenosine
                     5'-phosphosulfate synthase-like isoform X2"
                     /calculated_mol_wt=69929
     Region          25..219
                     /region_name="CysC"
                     /note="Adenylylsulfate kinase or related kinase [Inorganic
                     ion transport and metabolism]; COG0529"
                     /db_xref="CDD:440295"
     Region          248..617
                     /region_name="ATPS"
                     /note="ATP-sulfurylase; cd00517"
                     /db_xref="CDD:173895"
     Site            order(413..417,423,426,491,516..518,520..521,561..563)
                     /site_type="active"
                     /db_xref="CDD:173895"
     Site            420..423
                     /site_type="other"
                     /note="HXXH motif"
                     /db_xref="CDD:173895"
     Site            452..462
                     /site_type="other"
                     /note="flexible loop"
                     /db_xref="CDD:173895"
     CDS             1..623
                     /gene="LOC117608639"
                     /coded_by="XM_034334069.2:277..2148"
                     /db_xref="GeneID:117608639"
ORIGIN      
        1 msnetseipn krimttcsnv seqahhvsra krgqaigsvr gfrgctvwlt glsgagktsi
       61 afqveailvn hgiaaygldg dnvrsglncn lgfskedrke nirrvaevak lfadsgqicl
      121 csfvspfeed rqmarqihkm sdlpffeifv dtplnvcear dtkglykkar qgaikgftgi
      181 dqmyerpvnp dlvvttenct peesaatvid llekqciipv lqkptipire lfvpesriss
      241 gkaeattlqs leiseidvqw lqvlaegwaa pltgfmredq ylqtqhlkcl ldgdkevnqs
      301 vpivlaihte dkerldglsa ftlkyknkal ailrrpefyf hrkeercgwq fgtnnlghpy
      361 vkmihdsgdw lvggdievfk rirwhdgldk yrltpneirt kckkmkadav fafqlrnpih
      421 nghallmqdt kkhlleergf knpvlllhpl ggwtkdddvp lhtrilqhea vlsegvldsn
      481 stllaifpsp mmyagpvevq whakarmnag anfyivgrdp agiphpnktv tldgnlydpt
      541 hgarvlsiar glqdleiipf kvaaydkrhk kmsffdterk gdfefisgtk mrtlakvgen
      601 ppsgfmspka wsvlaqyyqn lek
//
LOCUS       XP_076549261             452 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 6-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076549261
VERSION     XP_076549261.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693146.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="coiled-coil domain-containing protein 6-like
                     isoform X1"
                     /calculated_mol_wt=51862
     Region          17..309
                     /region_name="DUF2046"
                     /note="Uncharacterized conserved protein H4 (DUF2046);
                     pfam09755"
                     /db_xref="CDD:401633"
     CDS             1..452
                     /gene="LOC143308078"
                     /coded_by="XM_076693146.1:186..1544"
                     /db_xref="GeneID:143308078"
ORIGIN      
        1 madrdsases dsssidggpi mmppspvtre qlqkrieslq qhnrvlkvel etyklrvkal
       61 qeenrglrqa sviiqakaeq eeefisntll kkiqalkkek etlahhyeqe eecltndlsr
      121 klnqlrqekc rleqtleqeq eclvnklmrk iekleaetla kqsnleqlrr ekvelentle
      181 qeqealvnkl wkrmdkleae krmlqikldq pvsdpaspre inngdtatsl sthiqtlrse
      241 varlrhtlli sqqehtekmq ryvneekqir eenlrlqrkl qleverreal crhlsesess
      301 lemeeerhyn eivmsgvnir nrtvsspvpy npspsssrpl spgnynnllf skyycnyfiq
      361 kfkyfyvili gsatregfnt nrcyacgqht tvpfassspp stahvvmsnr rtsdrfvkpa
      421 ipptivsptg ppiaanattn atggspmdit kt
//
LOCUS       XP_076549262             424 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 6-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076549262
VERSION     XP_076549262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693147.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..424
                     /product="coiled-coil domain-containing protein 6-like
                     isoform X2"
                     /calculated_mol_wt=48300
     Region          17..309
                     /region_name="DUF2046"
                     /note="Uncharacterized conserved protein H4 (DUF2046);
                     pfam09755"
                     /db_xref="CDD:401633"
     CDS             1..424
                     /gene="LOC143308078"
                     /coded_by="XM_076693147.1:183..1457"
                     /db_xref="GeneID:143308078"
ORIGIN      
        1 madrdsases dsssidggpi mmppspvtre qlqkrieslq qhnrvlkvel etyklrvkal
       61 qeenrglrqa sviiqakaeq eeefisntll kkiqalkkek etlahhyeqe eecltndlsr
      121 klnqlrqekc rleqtleqeq eclvnklmrk iekleaetla kqsnleqlrr ekvelentle
      181 qeqealvnkl wkrmdkleae krmlqikldq pvsdpaspre inngdtatsl sthiqtlrse
      241 varlrhtlli sqqehtekmq ryvneekqir eenlrlqrkl qleverreal crhlsesess
      301 lemeeerhyn eivmsgvnir nrtvsspvpy npspsssrpl spgsatregf ntnrcyacgq
      361 httvpfasss ppstahvvms nrrtsdrfvk paipptivsp tgppiaanat tnatggspmd
      421 itkt
//
LOCUS       XP_076549263             164 aa            linear   INV 03-SEP-2025
DEFINITION  guided entry of tail-anchored proteins factor 1-like [Osmia
            lignaria lignaria].
ACCESSION   XP_076549263
VERSION     XP_076549263.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693148.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..164
                     /product="guided entry of tail-anchored proteins factor
                     1-like"
                     /calculated_mol_wt=19145
     Region          3..150
                     /region_name="CHD5"
                     /note="CHD5-like protein; pfam04420"
                     /db_xref="CDD:427938"
     CDS             1..164
                     /gene="LOC143308079"
                     /coded_by="XM_076693148.1:131..625"
                     /db_xref="GeneID:143308079"
ORIGIN      
        1 mnlliistvs cvleyvipal ikyitsrfyt mnkqdielrn elinlkqemt gisivdefsk
       61 yaklqrrynk lestvkeqvn erlssrkkvq myltygfrll nglfmiilly qhknkpvitl
      121 pkgtlwpiqs llswpsyqed sislfmwlii aklvistckk idvt
//
LOCUS       XP_076549264             143 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143308080 [Osmia lignaria lignaria].
ACCESSION   XP_076549264
VERSION     XP_076549264.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693149.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="uncharacterized protein LOC143308080"
                     /calculated_mol_wt=16318
     Region          29..116
                     /region_name="Gar1"
                     /note="Gar1/Naf1 RNA binding region; pfam04410"
                     /db_xref="CDD:461296"
     CDS             1..143
                     /gene="LOC143308080"
                     /coded_by="XM_076693149.1:127..558"
                     /db_xref="GeneID:143308080"
ORIGIN      
        1 mdqinkclee lriqssgqhn ekqenyvagk innviktldi vivkpyssky llyfgsplfl
       61 sclnysdcks dyallgyidd ifgsiaepmy svsvkcdltk ildveasvyy fpnnsstthm
      121 yveymldeti nkekynirml ssi
//
LOCUS       XP_076549265             127 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear nucleic acid-binding protein C1D-like [Osmia lignaria
            lignaria].
ACCESSION   XP_076549265
VERSION     XP_076549265.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693150.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478281) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..127
                     /product="nuclear nucleic acid-binding protein C1D-like"
                     /calculated_mol_wt=14875
     Region          13..93
                     /region_name="Sas10_Utp3"
                     /note="Sas10/Utp3/C1D family; pfam04000"
                     /db_xref="CDD:461123"
     CDS             1..127
                     /gene="LOC143308081"
                     /coded_by="XM_076693150.1:211..594"
                     /db_xref="GeneID:143308081"
ORIGIN      
        1 mdadfeelsn dtnliarvtq fhdaamried tikmatdpei yeklsdedki qydllmsycl
       61 nstfwmylra egvdpakhqi rsenerlkks marakqisdr nslmprvnkd vakrfvrnsl
      121 weinkkk
//
LOCUS       XP_034174722            1560 aa            linear   INV 03-SEP-2025
DEFINITION  ral GTPase-activating protein subunit beta isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174722
VERSION     XP_034174722.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478306) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174722.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1560
                     /product="ral GTPase-activating protein subunit beta
                     isoform X1"
                     /calculated_mol_wt=172371
     Region          166..287
                     /region_name="RALGAPB_N"
                     /note="RALGAPB N-terminal domain; pfam20412"
                     /db_xref="CDD:466561"
     Region          1240..>1323
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     CDS             1..1560
                     /gene="LOC117601705"
                     /coded_by="XM_034318831.2:432..5114"
                     /db_xref="GeneID:117601705"
ORIGIN      
        1 mnlgvfnrvn lkdsqggmys ewaslsvliq qgseesqsvl ekfspgagke valsivrqla
       61 anlgitqaae psplctdkev qwcmevicfg lslplaehdt vrdcvnvyce wlsalystpk
      121 icvpkpiidd pnfyarkiis hfhnlfvprk gevwpflyqd lgtdtinrqa vlchrvlrtl
      181 qqiargpatl eretweslll fligindall appatredag eqlcervlgv llevwlvace
      241 rnfpspplwr tlresclrwr hrlalveqwn rvclaltarl lqimygpmfp elkiseedsq
      301 lvpptmsdea vaqawyrllr tigdpvdlcr pavisqtqaf lqyaiaspnv idpcqhpclq
      361 slpqiflkai kgiagqidaf lvtnvlgvsq accweeccvs titsgsastg sggmgwdkas
      421 nkdltqpspt pptqrrlaks fsvtpsavtk gipkasligl ttsrvsstpp mlmsnsgpss
      481 assttsmtsl vqdirpplap grpkcnsilh lfgewlfeaa figtdgwsqn lpqpsgatkr
      541 pssvlvdgps slqenvndvp palcidryea graealgalc rifcakktge eifpvylarf
      601 yqamnhglkv desrecgetl ssiilnsadl frldlngvqv lvpamisale tvlpekdlkl
      661 ksnvvskpdl rrasihllis mlalplhfqn ltikelptfa nsviteknhv tfaqlksrlm
      721 nllinalqve tdpqnthmll galllsvqds aaaeeveqvt qpdtmahdsa vnllssvtsd
      781 sasqisissd qrslgetsdi ttlqeecvaf dsahalfvra tylvchrlis swktdlnisl
      841 aaleilsgla rtriretdal eckravkwlc dyiayqcwrp ppahskdlhs sivaafsclt
      901 twltahpqll qdkdclttvl evvelgvsgt ksvgkpgepi kmkdekelkp asmrvrdaad
      961 alltvileqv gyfpsacgaq slsslldevs llrhcnswtg grvprqaave rfryfvaena
     1021 tilalleepl gndqdpqptv tvlirgpfgr hawtlqlrhl prhrssirsv ntnpgrplpl
     1081 aeaaprtdyk prffpdnvdr iphckvdesi psleavmndn dvirsehqil sqllerqmni
     1141 etkycdgydk vpdekpdecv ppqichefqt arlflshfgf lnmepkende srngsltald
     1201 ptipgfsldl etldnisprt cdtvyifyvk agqkssteil snvlheanvs tnfleflnsl
     1261 gwpvsvsaha gwtghvstsw rvtpqlnvpq pahsdhggal yngdthvlyw advsseiafv
     1321 vptqsnnmls sdsleetsfg sdistnqvwf ersisestss rncgtgqntv qnsramsldl
     1381 ekqppsltgs npsnsssadp vkprratkqv lpvqtdikim vvwlesledy aqfpigdllp
     1441 ctytgleqsr viqasdvqvi fiqalssglm rvrlqgpvsr inlatplidg mvvsrrvlgt
     1501 lvrqtalnmg rrkrldndsy hpphvrrrlk iqemvqkyrk nltdpelltl lfsstqtyqv
//
LOCUS       XP_034174726            1555 aa            linear   INV 03-SEP-2025
DEFINITION  ral GTPase-activating protein subunit beta isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174726
VERSION     XP_034174726.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318835.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478306) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174726.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1555
                     /product="ral GTPase-activating protein subunit beta
                     isoform X2"
                     /calculated_mol_wt=171844
     Region          166..287
                     /region_name="RALGAPB_N"
                     /note="RALGAPB N-terminal domain; pfam20412"
                     /db_xref="CDD:466561"
     Region          1235..>1318
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     CDS             1..1555
                     /gene="LOC117601705"
                     /coded_by="XM_034318835.2:432..5099"
                     /db_xref="GeneID:117601705"
ORIGIN      
        1 mnlgvfnrvn lkdsqggmys ewaslsvliq qgseesqsvl ekfspgagke valsivrqla
       61 anlgitqaae psplctdkev qwcmevicfg lslplaehdt vrdcvnvyce wlsalystpk
      121 icvpkpiidd pnfyarkiis hfhnlfvprk gevwpflyqd lgtdtinrqa vlchrvlrtl
      181 qqiargpatl eretweslll fligindall appatredag eqlcervlgv llevwlvace
      241 rnfpspplwr tlresclrwr hrlalveqwn rvclaltarl lqimygpmfp elkiseedsq
      301 lvpptmsdea vaqawyrllr tigdpvdlcr pavisqtqaf lqyaiaspnv idpcqhpclq
      361 slpqiflkai kgiagqidaf lgvsqaccwe eccvstitsg sastgsggmg wdkasnkdlt
      421 qpsptpptqr rlaksfsvtp savtkgipka sliglttsrv sstppmlmsn sgpssasstt
      481 smtslvqdir pplapgrpkc nsilhlfgew lfeaafigtd gwsqnlpqps gatkrpssvl
      541 vdgpsslqen vndvppalci dryeagraea lgalcrifca kktgeeifpv ylarfyqamn
      601 hglkvdesre cgetlssiil nsadlfrldl ngvqvlvpam isaletvlpe kdlklksnvv
      661 skpdlrrasi hllismlalp lhfqnltike lptfansvit eknhvtfaql ksrlmnllin
      721 alqvetdpqn thmllgalll svqdsaaaee veqvtqpdtm ahdsavnlls svtsdsasqi
      781 sissdqrslg etsdittlqe ecvafdsaha lfvratylvc hrlisswktd lnislaalei
      841 lsglartrir etdaleckra vkwlcdyiay qcwrpppahs kdlhssivaa fsclttwlta
      901 hpqllqdkdc lttvlevvel gvsgtksvgk pgepikmkde kelkpasmrv rdaadalltv
      961 ileqvgyfps acgaqslssl ldevsllrhc nswtggrvpr qaaverfryf vaenatilal
     1021 leeplgndqd pqptvtvlir gpfgrhawtl qlrhlprhrs sirsvntnpg rplplaeaap
     1081 rtdykprffp dnvdriphck vdesipslea vmndndvirs ehqilsqlle rqmnietkyc
     1141 dgydkvpdek pdecvppqic hefqtarlfl shfgflnmep kendesrngs ltaldptipg
     1201 fsldletldn isprtcdtvy ifyvkagqks steilsnvlh eanvstnfle flnslgwpvs
     1261 vsahagwtgh vstswrvtpq lnvpqpahsd hggalyngdt hvlywadvss eiafvvptqs
     1321 nnmlssdsle etsfgsdist nqvwfersis estssrncgt gqntvqnsra msldlekqpp
     1381 sltgsnpsns ssadpvkprr atkqvlpvqt dikimvvwle sledyaqfpi gdllpctytg
     1441 leqsrviqas dvqvifiqal ssglmrvrlq gpvsrinlat plidgmvvsr rvlgtlvrqt
     1501 alnmgrrkrl dndsyhpphv rrrlkiqemv qkyrknltdp elltllfsst qtyqv
//
LOCUS       XP_034174727            1551 aa            linear   INV 03-SEP-2025
DEFINITION  ral GTPase-activating protein subunit beta isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174727
VERSION     XP_034174727.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318836.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478306) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174727.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1551
                     /product="ral GTPase-activating protein subunit beta
                     isoform X3"
                     /calculated_mol_wt=171208
     Region          157..278
                     /region_name="RALGAPB_N"
                     /note="RALGAPB N-terminal domain; pfam20412"
                     /db_xref="CDD:466561"
     Region          1231..>1314
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     CDS             1..1551
                     /gene="LOC117601705"
                     /coded_by="XM_034318836.2:432..5087"
                     /db_xref="GeneID:117601705"
ORIGIN      
        1 mnlgvfnrvn lkdsqggmys ewaslsvliq qgseesqsvl ekfspgagke valsivrqla
       61 anlgitqaae psplctdkev qwcmevicfg lslplaehdt vrdcvnvyce wlsalystpk
      121 icvpkpiidd pnfyarkiis hfhnlfvprk gegtdtinrq avlchrvlrt lqqiargpat
      181 leretwesll lfligindal lappatreda geqlcervlg vllevwlvac ernfpspplw
      241 rtlresclrw rhrlalveqw nrvclaltar llqimygpmf pelkiseeds qlvpptmsde
      301 avaqawyrll rtigdpvdlc rpavisqtqa flqyaiaspn vidpcqhpcl qslpqiflka
      361 ikgiagqida flvtnvlgvs qaccweeccv stitsgsast gsggmgwdka snkdltqpsp
      421 tpptqrrlak sfsvtpsavt kgipkaslig lttsrvsstp pmlmsnsgps sassttsmts
      481 lvqdirppla pgrpkcnsil hlfgewlfea afigtdgwsq nlpqpsgatk rpssvlvdgp
      541 sslqenvndv ppalcidrye agraealgal crifcakktg eeifpvylar fyqamnhglk
      601 vdesrecget lssiilnsad lfrldlngvq vlvpamisal etvlpekdlk lksnvvskpd
      661 lrrasihlli smlalplhfq nltikelptf ansviteknh vtfaqlksrl mnllinalqv
      721 etdpqnthml lgalllsvqd saaaeeveqv tqpdtmahds avnllssvts dsasqisiss
      781 dqrslgetsd ittlqeecva fdsahalfvr atylvchrli sswktdlnis laaleilsgl
      841 artriretda leckravkwl cdyiayqcwr pppahskdlh ssivaafscl ttwltahpql
      901 lqdkdclttv levvelgvsg tksvgkpgep ikmkdekelk pasmrvrdaa dalltvileq
      961 vgyfpsacga qslsslldev sllrhcnswt ggrvprqaav erfryfvaen atilalleep
     1021 lgndqdpqpt vtvlirgpfg rhawtlqlrh lprhrssirs vntnpgrplp laeaaprtdy
     1081 kprffpdnvd riphckvdes ipsleavmnd ndvirsehqi lsqllerqmn ietkycdgyd
     1141 kvpdekpdec vppqichefq tarlflshfg flnmepkend esrngsltal dptipgfsld
     1201 letldnispr tcdtvyifyv kagqksstei lsnvlheanv stnfleflns lgwpvsvsah
     1261 agwtghvsts wrvtpqlnvp qpahsdhgga lyngdthvly wadvsseiaf vvptqsnnml
     1321 ssdsleetsf gsdistnqvw fersisests srncgtgqnt vqnsramsld lekqppsltg
     1381 snpsnsssad pvkprratkq vlpvqtdiki mvvwlesled yaqfpigdll pctytgleqs
     1441 rviqasdvqv ifiqalssgl mrvrlqgpvs rinlatplid gmvvsrrvlg tlvrqtalnm
     1501 grrkrldnds yhpphvrrrl kiqemvqkyr knltdpellt llfsstqtyq v
//
LOCUS       XP_076549267            1526 aa            linear   INV 03-SEP-2025
DEFINITION  ral GTPase-activating protein subunit beta isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076549267
VERSION     XP_076549267.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693152.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478306) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1526
                     /product="ral GTPase-activating protein subunit beta
                     isoform X4"
                     /calculated_mol_wt=168783
     Region          166..287
                     /region_name="RALGAPB_N"
                     /note="RALGAPB N-terminal domain; pfam20412"
                     /db_xref="CDD:466561"
     Region          1206..>1289
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     CDS             1..1526
                     /gene="LOC117601705"
                     /coded_by="XM_076693152.1:432..5012"
                     /db_xref="GeneID:117601705"
ORIGIN      
        1 mnlgvfnrvn lkdsqggmys ewaslsvliq qgseesqsvl ekfspgagke valsivrqla
       61 anlgitqaae psplctdkev qwcmevicfg lslplaehdt vrdcvnvyce wlsalystpk
      121 icvpkpiidd pnfyarkiis hfhnlfvprk gevwpflyqd lgtdtinrqa vlchrvlrtl
      181 qqiargpatl eretweslll fligindall appatredag eqlcervlgv llevwlvace
      241 rnfpspplwr tlresclrwr hrlalveqwn rvclaltarl lqimygpmfp elkiseedsq
      301 lvpptmsdea vaqawyrllr tigdpvdlcr pavisqtqaf lqyaiaspnv idpcqhpclq
      361 slpqiflkai kgiagqidaf lvtnvlgvsq accweeccvs titsgsastg sggmgwdkas
      421 nkdltqpspt pptqrrlaks fsvtpsavtk gipkasligl ttsrvsstpp mlmsnsgpss
      481 assttsmtsl vqdirpplap grpkcnsilh lfgewlfeaa figtdgwsqn lpqpsgatkr
      541 pssvlvdgps slqenvndvp palcidryea graealgalc rifcakktge eifpvylarf
      601 yqamnhglkv desrecgetl ssiilnsadl frldlngvqv lvpamisale tvlpekdlkl
      661 ksnvvskpdl rrasihllis mlalplhfqn ltikelptfa nsviteknhv tfaqlksrlm
      721 nllinalqve tdpqnthmll galllsvqds aaaeeveqvt qpdtmahdsa vnllssdsah
      781 alfvratylv chrlisswkt dlnislaale ilsglartri retdaleckr avkwlcdyia
      841 yqcwrpppah skdlhssiva afsclttwlt ahpqllqdkd clttvlevve lgvsgtksvg
      901 kpgepikmkd ekelkpasmr vrdaadallt vileqvgyfp sacgaqslss lldevsllrh
      961 cnswtggrvp rqaaverfry fvaenatila lleeplgndq dpqptvtvli rgpfgrhawt
     1021 lqlrhlprhr ssirsvntnp grplplaeaa prtdykprff pdnvdriphc kvdesipsle
     1081 avmndndvir sehqilsqll erqmnietky cdgydkvpde kpdecvppqi chefqtarlf
     1141 lshfgflnme pkendesrng sltaldptip gfsldletld nisprtcdtv yifyvkagqk
     1201 ssteilsnvl heanvstnfl eflnslgwpv svsahagwtg hvstswrvtp qlnvpqpahs
     1261 dhggalyngd thvlywadvs seiafvvptq snnmlssdsl eetsfgsdis tnqvwfersi
     1321 sestssrncg tgqntvqnsr amsldlekqp psltgsnpsn sssadpvkpr ratkqvlpvq
     1381 tdikimvvwl esledyaqfp igdllpctyt gleqsrviqa sdvqvifiqa lssglmrvrl
     1441 qgpvsrinla tplidgmvvs rrvlgtlvrq talnmgrrkr ldndsyhpph vrrrlkiqem
     1501 vqkyrknltd pelltllfss tqtyqv
//
LOCUS       XP_076549268            1491 aa            linear   INV 03-SEP-2025
DEFINITION  ral GTPase-activating protein subunit beta isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_076549268
VERSION     XP_076549268.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693153.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478306) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1491
                     /product="ral GTPase-activating protein subunit beta
                     isoform X5"
                     /calculated_mol_wt=165283
     Region          166..287
                     /region_name="RALGAPB_N"
                     /note="RALGAPB N-terminal domain; pfam20412"
                     /db_xref="CDD:466561"
     Region          1171..>1254
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     CDS             1..1491
                     /gene="LOC117601705"
                     /coded_by="XM_076693153.1:432..4907"
                     /db_xref="GeneID:117601705"
ORIGIN      
        1 mnlgvfnrvn lkdsqggmys ewaslsvliq qgseesqsvl ekfspgagke valsivrqla
       61 anlgitqaae psplctdkev qwcmevicfg lslplaehdt vrdcvnvyce wlsalystpk
      121 icvpkpiidd pnfyarkiis hfhnlfvprk gevwpflyqd lgtdtinrqa vlchrvlrtl
      181 qqiargpatl eretweslll fligindall appatredag eqlcervlgv llevwlvace
      241 rnfpspplwr tlresclrwr hrlalveqwn rvclaltarl lqimygpmfp elkiseedsq
      301 lvpptmsdea vaqawyrllr tigdpvdlcr pavisqtqaf lqyaiaspnv idpcqhpclq
      361 slpqiflkai kgiagqidaf lgipkaslig lttsrvsstp pmlmsnsgps sassttsmts
      421 lvqdirppla pgrpkcnsil hlfgewlfea afigtdgwsq nlpqpsgatk rpssvlvdgp
      481 sslqenvndv ppalcidrye agraealgal crifcakktg eeifpvylar fyqamnhglk
      541 vdesrecget lssiilnsad lfrldlngvq vlvpamisal etvlpekdlk lksnvvskpd
      601 lrrasihlli smlalplhfq nltikelptf ansviteknh vtfaqlksrl mnllinalqv
      661 etdpqnthml lgalllsvqd saaaeeveqv tqpdtmahds avnllssvts dsasqisiss
      721 dqrslgetsd ittlqeecva fdsahalfvr atylvchrli sswktdlnis laaleilsgl
      781 artriretda leckravkwl cdyiayqcwr pppahskdlh ssivaafscl ttwltahpql
      841 lqdkdclttv levvelgvsg tksvgkpgep ikmkdekelk pasmrvrdaa dalltvileq
      901 vgyfpsacga qslsslldev sllrhcnswt ggrvprqaav erfryfvaen atilalleep
      961 lgndqdpqpt vtvlirgpfg rhawtlqlrh lprhrssirs vntnpgrplp laeaaprtdy
     1021 kprffpdnvd riphckvdes ipsleavmnd ndvirsehqi lsqllerqmn ietkycdgyd
     1081 kvpdekpdec vppqichefq tarlflshfg flnmepkend esrngsltal dptipgfsld
     1141 letldnispr tcdtvyifyv kagqksstei lsnvlheanv stnfleflns lgwpvsvsah
     1201 agwtghvsts wrvtpqlnvp qpahsdhgga lyngdthvly wadvsseiaf vvptqsnnml
     1261 ssdsleetsf gsdistnqvw fersisests srncgtgqnt vqnsramsld lekqppsltg
     1321 snpsnsssad pvkprratkq vlpvqtdiki mvvwlesled yaqfpigdll pctytgleqs
     1381 rviqasdvqv ifiqalssgl mrvrlqgpvs rinlatplid gmvvsrrvlg tlvrqtalnm
     1441 grrkrldnds yhpphvrrrl kiqemvqkyr knltdpellt llfsstqtyq v
//
LOCUS       XP_076549269            1174 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143308146 [Osmia lignaria lignaria].
ACCESSION   XP_076549269
VERSION     XP_076549269.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693154.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478306) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1174
                     /product="uncharacterized protein LOC143308146"
                     /calculated_mol_wt=130256
     Region          621..983
                     /region_name="WAPL"
                     /note="Wings apart-like protein regulation of
                     heterochromatin; pfam07814"
                     /db_xref="CDD:462278"
     CDS             1..1174
                     /gene="LOC143308146"
                     /coded_by="XM_076693154.1:418..3942"
                     /db_xref="GeneID:143308146"
ORIGIN      
        1 mtsrsytksy srkvssmtpg siqfdklfre nsnrpsaaks agtvgkwgit sftsirstni
       61 ngrrddihnt fgakrikldy gnqntrvnsg kdpfsfetdp dnksdvappv nkpkkffksr
      121 nvpptveesr qdvaiyrqvp dshygkartq kncpqppakq tltkvssipp kkvadtldgt
      181 nndppsreeg kppivlrick gtarlvcgnv qnaqepepet yristplisp tkvdiepknf
      241 nvdtlktdhk tvrphepiis tvsiplense mrrttrsrak nlhleltttt tattttsvms
      301 tasvtpsshs sglsltlrks vtdsnntlis hydivktdcs stysckptie pligrdqplp
      361 tttqelidil ssdsdpmqtr piminasete enaeavqnev qhcsidiptn evvpkptsep
      421 epepeheaep epepepelep dteteveqpt rtsdndtata ktlvdqdwfs gsddsegvsg
      481 naesdvalhv tstvtesqsl napcttvqkp atkkgsifks rstgatngnk rralykhkwc
      541 dsdkesnttd ttntgnstpt iasgshggtg pvaygeefda skltrvvtys evdadfades
      601 dvvtsvrcgk kvkgfytvvk nvkkahqiqe sgefqefndd veyildtlre nnpnatrcls
      661 airlaskcma pafrmhvrah gtvakffkal hdatkdqsla lctatvmfvl sqdrlamdld
      721 rdclelmlsl lesdashkda lddcglsrae llknkekvrq lcadiqaqgh akhlnldnit
      781 vgqlametll sltskragew fkeelrelgg lehivktird chrhintqdi trsgwtdpll
      841 dklrkvdrcl rvlenvtdmn eenqvyllky engilvstla nlyhlcgqei piyptidssd
      901 ktstgavire clfaiikvli nlthrfnrqs fgskslgsqp gvldcslyll lrvpeslpee
      961 krfdmmmlal illinlveqc ddnkqllies kappstedif daeqsgvecl idlfykqeel
     1021 araeeqktda ildgkkdseq tetvstttks qeefieetvt kllqkagrhm ehtligayvv
     1081 lllgylimdn kdyeslvrsr lpdnnfttmv svlqkffnfm nltasnevss cgiaatekvi
     1141 kflkisdari ddeknelpls aledsnmmlh lsss
//
LOCUS       XP_076549270             981 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143308190 [Osmia lignaria lignaria].
ACCESSION   XP_076549270
VERSION     XP_076549270.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693155.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478321) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..981
                     /product="uncharacterized protein LOC143308190"
                     /calculated_mol_wt=108771
     CDS             1..981
                     /gene="LOC143308190"
                     /coded_by="XM_076693155.1:244..3189"
                     /db_xref="GeneID:143308190"
ORIGIN      
        1 midtgaepni vksgilstnt avdttgalki tgitngyvkt lgsteisiyg ktatfqvvsd
       61 dfpipmdgil gaaflndgae isyvkqvvlw kgtelpfvnk kltgiktiae ivsrqelnsc
      121 vttsdkrvdk dhrptvrcvs atrkhesmth eplvrpepss sddenknknn plissrkkvr
      181 heplirscsc trenedkpsq spstssspki sssqnkvkst qtkiklsfnk wlfmydslhk
      241 glfsminqls rdnkiqivsh kplwndsldi hnvnteiarg flrvnikgir fkrnqtppfn
      301 dkrlqkgqps nepqqlkmnp tsgmvllall fgiapahali gydcggrtln vttlsavdpi
      361 dcepvdaepa tqstniqllq lgdynsapvi qckveidrsi yycgmhshts vvqngrqqyi
      421 fqtsreacrt lhatgsiais ssvqisgvgr nssvtfsltl agtitmdgsc tgtsyadpfg
      481 swdnvvvqgl vkitvqsypa tvklstgqii lksgyqcrlq eevcldpeag htywdsipkd
      541 ycefskynvl yegkatkiip hgsngpllyt vmadattfal atthnstvcg ytlvktehpk
      601 lliletdkng klprtqptsv enldiftyvn skfiyvekhi rtqlsqlykd vvmqrchler
      661 qviqnalali hvapeemala itkqpghmai sagevihiik cipvscrlrr tkhchnelpv
      721 tyknssyflt pktriltsag trkdcsslip tmykihnswy ritprvveai ppqrmqsitq
      781 ptwkyvdpan latggiyspe ditrlrdhim fpiektavin tlvqgvtgrq yaqgnvqlsh
      841 lldeasinri aesaarkfwn gfitfgsvts gviglyvavq airliittil ngialhaayg
      901 wsmrlvaalw ssmsyfcifl kkrkeelntg greclpqksd napstplsei hipisertpt
      961 rplversprp rllfpvtrde p
//
LOCUS       XP_034195295             460 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611454 [Osmia lignaria lignaria].
ACCESSION   XP_034195295
VERSION     XP_034195295.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339404.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478333) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195295.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="uncharacterized protein LOC117611454"
                     /calculated_mol_wt=52050
     Region          143..263
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..460
                     /gene="LOC117611454"
                     /coded_by="XM_034339404.2:1..1383"
                     /db_xref="GeneID:117611454"
ORIGIN      
        1 mprstdqysn qtqstshggn lsalrrrrgn iighittfvr lienmqqseq hdigllrahl
       61 dslkdvwsrf deiqfeleti desetsrrye ihndyvtvla ratsiidgan attslrrntt
      121 dssatsvsap maiklpemrl ptfdgkyenw tsyfdnfssm idqnvdltpt qklqylrstl
      181 tgkaaaciqs lsttdanyad aidllrnkfd ckrrillkhc daiqaipkls tdsseglgdl
      241 vdtvrqnlrs lknlgidtss wdciiisiil skinadtawh welslkdkqm paythlldfl
      301 ekrancgpti hkkssttptq fgrntstrlp syrpstrnha fitainsrdr qshresytsp
      361 sastnfpkcp lchdvhgiwr cekfyglsie artaairras lcpnclrhdh npeicrkrsc
      421 qicyrhhhtl lhkssqpsnq ttpppvkrak ttdsqveptd
//
LOCUS       XP_076549271             929 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143308200 [Osmia lignaria lignaria].
ACCESSION   XP_076549271
VERSION     XP_076549271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693156.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478333) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 92% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..929
                     /product="uncharacterized protein LOC143308200"
                     /calculated_mol_wt=105945
     Region          <1..147
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            37
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          163..323
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          539..589
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          634..728
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          818..909
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..929
                     /gene="LOC143308200"
                     /coded_by="XM_076693156.1:1..2790"
                     /db_xref="GeneID:143308200"
ORIGIN      
        1 myrqffvrke drkyqrilwr dntnrictye lktvtfglsa apylavrclt qlaqdeghkf
       61 phaaevllrd fyvddaltga stieealslr edltlllrsa glnirqwatn hkallkglpe
      121 qsinkklhlg esstlktlgi vwkssedsit yevktqtvtt hitkrlisse iakiydplgl
      181 lgpviinaki llqrlwsikv dwdeslpmdi htewmnyykq lpllnniifs rktiidsptk
      241 lewhgfcgas ekaygaclyi rstnasghth tellfakskv aplktqsipr lelcgalllt
      301 slintannal hveihhttlw tdstivlswl rtsphllktf vanrvaeiqt ktditnwrhv
      361 pttdnpadli srgqapkefl qpsiwhhgpe wlareesfwp tdnvplcetv peqktvvclt
      421 ttsidtsvfd nysswgkmqr ivarclrwkk tntnkgsltv selknahdil ikilqgihfs
      481 kelrcisnke peiggklqrl npfidkdgil rvggrlkhss ipfsqrhpii lpktrvtslm
      541 ieaehraqlh tgvqntlyav rrrywpidgr sqvwkairtc trclraqppp vnyimgnlpe
      601 arviesrpft htgvdycgpf yikerkhrnr trvkvyvavf vclavkavhl elvsdlttet
      661 flaalrrfia rrgfckrlys dngtnfigay nelrevrell ksndhnqkvr tfladrsiew
      721 sfipphaphf gglweaavka fkhhltrvvs telttfenln tliieiesil nsrpltpisa
      781 dandllaltp ghfligdslt slrehdftdt ptnrlsswqh vqqmkqhfwn rwhreylnel
      841 aqrskwskgs hpikegtlvl lrddntpsmq walgrvtkvh pgsdgiiraa tiktatgtld
      901 rstkglvplp yqaeeddrip gdptpmdks
//
LOCUS       XP_076549272             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143308216 [Osmia lignaria lignaria].
ACCESSION   XP_076549272
VERSION     XP_076549272.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693157.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478346) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC143308216"
                     /calculated_mol_wt=57394
     Region          2..>72
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <278..>411
                     /region_name="Enamelin"
                     /note="pfam15362"
                     /db_xref="CDD:464672"
     CDS             1..490
                     /gene="LOC143308216"
                     /coded_by="XM_076693157.1:1..1473"
                     /db_xref="GeneID:143308216"
ORIGIN      
        1 manretrstr qtdttarete llayeqslae rehhvreter rieaerleii naqkekdnlr
       61 aeleklrrdm qeatvqnper vptastmpms esprresaes sdftlkeavn tipifdgnni
      121 svlqfcracr rakeivpprv ertlarlilt rlrgraalai edefcetvat vcnrlkdife
      181 prrsvdhfrg emanifmkpn ehildfigrv kdlrdaildc nrqspnianv ddltvdsfin
      241 glnptlraei rpfrnaplih vfdeairayk qseldrvryg ksttdtrrvq fantyesrda
      301 rspspyrsys pqntwrrspt rdyrryespe parynsyrde nrvaprrdsy reenryrete
      361 yretirqapr rneprvsspp ryvppfrreq spqptrtyes nnqknnviak kscsycqrig
      421 hdvhecqkra fvendyrrns nansnkintk inkisnsgnd rsltpnrdrq revspspihr
      481 istatetknn
//
LOCUS       XP_076549273             584 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611486, partial [Osmia lignaria
            lignaria].
ACCESSION   XP_076549273
VERSION     XP_076549273.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693158.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478350) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..>584
                     /product="uncharacterized protein LOC117611486"
     Region          <1..150
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            38
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          165..324
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..584
                     /gene="LOC117611486"
                     /coded_by="XM_076693158.1:1..>1752"
                     /db_xref="GeneID:117611486"
ORIGIN      
        1 mfrqirvape dtkyqcifwr psensplaay rletvtygta capfhanrvl kqlakdegek
       61 yplgaailld efyvddamfg addpsllvdm rdelnrllma ggfklrkwas nsssllkdip
      121 aedhglavsr tlqddervkv lgiqwnpssd vfhfhtntpe lkkytkrafl sllaktydpl
      181 gwiapfivqg kiilqklwla kvswddnlpp dlardcekfh smlsytrdis iprwvgvgge
      241 iktvqlhgfc dashlayaaa vyirvessst kvtllyaktk vaplkvqsip rlelcaaell
      301 srainfvlga lrvkldaivc wsdskvvldw lnappgnwkt fvanrvsevq tslpsalwrh
      361 vpgtqnpadc asrgcsardl rthdlwwsgp nwlsdpsesw pqqgftprde qvgeecsvqv
      421 asnvdhkvep leilsrfssw pkllrvlvyv ykfynacrks pntekgkvvs kteiqfaktk
      481 cfqliqanhf ptelrelakp kgvltkssil alspyldkdh iirvggrlky splpesekhp
      541 iilprchvts lilkhvhimt qhgglqltlr tlrqqywvih artq
//
LOCUS       XP_076549274             184 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: NADH-ubiquinone oxidoreductase chain 1-like,
            partial [Osmia lignaria lignaria].
ACCESSION   XP_076549274
VERSION     XP_076549274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693159.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478358) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            internal stop codons :: corrected 2 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..184
                     /product="LOW QUALITY PROTEIN: NADH-ubiquinone
                     oxidoreductase chain 1-like"
     Region          <1..>131
                     /region_name="PRK06076"
                     /note="NADH-quinone oxidoreductase subunit NuoH; cl00469"
                     /db_xref="CDD:469782"
     CDS             1..184
                     /gene="LOC143308227"
                     /coded_by="XM_076693159.1:<1..555"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 2 bases at 2
                     genomic stop codons"
                     /db_xref="GeneID:143308227"
ORIGIN      
        1 fkfglvflfl ilslrvypti iigxssnsiy ailgclrsis qsisfevrlf fiffviiifi
       61 erfsletfik fqfnvkfiff ffplyfilii riiielnrap fdliegesel vsgfnveyyr
      121 grfslifiae yiiilfirli isiiffnfny siftlliily hnfffixirr iiprirydel
      181 iyic
//
LOCUS       XP_076549275             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143308231 [Osmia lignaria lignaria].
ACCESSION   XP_076549275
VERSION     XP_076549275.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076693160.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NW_027478365) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="Unknown"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC143308231"
                     /calculated_mol_wt=36906
     CDS             1..320
                     /gene="LOC143308231"
                     /coded_by="XM_076693160.1:70..1032"
                     /db_xref="GeneID:143308231"
ORIGIN      
        1 mkavlfrerq rnyphiptsl tvlnecivnh sfmrnicktv ikdenakvai lfstetllqi
       61 lnvsqtiyad gtfsvlparp piaqlyiihi qymdtgvgtv filcealtka mytaiwckmk
      121 elapmlhknl kftmtdyeta amvtleqqfs ssivkgcwfh ynqallrkwr hlsltdaptk
      181 vlsmamtlal vstdlfeqgf letereaasf iseyvaikil ieyirstwlp kaekvsvndc
      241 pmrtnnitec ynnivllkmg kgkkniwvfl ekleqlimde eikikrlneg qcvrhksnkk
      301 tiirnkriet iqkdlanrrk
//
LOCUS       XP_034195103             467 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase MINDY-3 homolog [Osmia
            lignaria lignaria].
ACCESSION   XP_034195103
VERSION     XP_034195103.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339212.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195103.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="ubiquitin carboxyl-terminal hydrolase MINDY-3
                     homolog"
                     /calculated_mol_wt=53012
     Region          16..323
                     /region_name="MINDY-3_4_CD"
                     /note="Deubiquitinating enzyme MINDY-3/4, conserved
                     domain; pfam13898"
                     /db_xref="CDD:464029"
     CDS             1..467
                     /gene="LOC117611266"
                     /coded_by="XM_034339212.2:203..1606"
                     /db_xref="GeneID:117611266"
ORIGIN      
        1 maenvvqgde lirsiktllw gnsvkedvfk rwaqgfyfsp destaliqek ggpcaviapv
       61 qayilkvlls etdisnwrni nqeknchllv qasveilkqa agekvpkfvi vymdcklsnk
      121 nnegesrlsg nvskteheee knvenkenee eneelmtkdv neecmtmesd sfhsqlrlft
      181 tntseqveef llerlemlka kygillllys vigtkgisei csemsdpses midstygygn
      241 qslinlmltg ravshvwdhd qdigglklrg idkqntigfl allehlcyce vgtflkspsy
      301 piwvlgseth ltvlfsnenr lvspetpadq akrifrkfdp egnnfipanl lqdvlaelgl
      361 vtdpeyvnvm rkkldsenlg iilrnnfmde ffpeeprtcp dtfplyhyng lrhsnpnnkv
      421 myhkgqavll ectikaimes npmltvlqtk wprieiqwdi gqnpsln
//
LOCUS       XP_034195104             338 aa            linear   INV 03-SEP-2025
DEFINITION  ribonuclease H2 subunit A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195104
VERSION     XP_034195104.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339213.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195104.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="ribonuclease H2 subunit A isoform X1"
                     /calculated_mol_wt=38155
     Region          69..286
                     /region_name="RNase_HII_eukaryota_like"
                     /note="Eukaryotic RNase HII; cd07181"
                     /db_xref="CDD:260002"
     Site            order(72..77,107,151..153,180..182,206,208,222,250,
                     275..277)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260002"
     Site            order(138,141..142,262..265,267,273,282,286)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:260002"
     CDS             1..338
                     /gene="LOC117611267"
                     /coded_by="XM_034339213.2:122..1138"
                     /db_xref="GeneID:117611267"
ORIGIN      
        1 mhtgfehkra imdneketks eencsspeed vfitcrgdlt syfedwdhsi nkvylskipq
       61 ickdepcqlg ideagrgpvl gpmlygisya plsmkqllvd lgcadskslt eekrdvifdk
      121 icekrdkmgw aveaispnfi ansmyrrskt slnevsmtsa ieltkkiiea gawisevyvd
      181 tvgrpekyqa kleqifpgik itvakkadst ypivsaasic akvsrdhair awqflegsia
      241 keygsgypnd pdtknwliqn vdpvfgfpql vrfswstaek ileekalsve weevedggnp
      301 geqkisnfff rsppkscqsk rkrykffadr clfntttl
//
LOCUS       XP_034195105             327 aa            linear   INV 03-SEP-2025
DEFINITION  ribonuclease H2 subunit A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195105
VERSION     XP_034195105.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339214.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195105.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="ribonuclease H2 subunit A isoform X2"
                     /calculated_mol_wt=36846
     Region          58..275
                     /region_name="RNase_HII_eukaryota_like"
                     /note="Eukaryotic RNase HII; cd07181"
                     /db_xref="CDD:260002"
     Site            order(61..66,96,140..142,169..171,195,197,211,239,
                     264..266)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260002"
     Site            order(127,130..131,251..254,256,262,271,275)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:260002"
     CDS             1..327
                     /gene="LOC117611267"
                     /coded_by="XM_034339214.2:169..1152"
                     /db_xref="GeneID:117611267"
ORIGIN      
        1 mdneketkse encsspeedv fitcrgdlts yfedwdhsin kvylskipqi ckdepcqlgi
       61 deagrgpvlg pmlygisyap lsmkqllvdl gcadskslte ekrdvifdki cekrdkmgwa
      121 veaispnfia nsmyrrskts lnevsmtsai eltkkiieag awisevyvdt vgrpekyqak
      181 leqifpgiki tvakkadsty pivsaasica kvsrdhaira wqflegsiak eygsgypndp
      241 dtknwliqnv dpvfgfpqlv rfswstaeki leekalsvew eevedggnpg eqkisnfffr
      301 sppkscqskr krykffadrc lfntttl
//
LOCUS       XP_034195107             327 aa            linear   INV 03-SEP-2025
DEFINITION  ribonuclease H2 subunit A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195107
VERSION     XP_034195107.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339216.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195107.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="ribonuclease H2 subunit A isoform X2"
                     /calculated_mol_wt=36846
     Region          58..275
                     /region_name="RNase_HII_eukaryota_like"
                     /note="Eukaryotic RNase HII; cd07181"
                     /db_xref="CDD:260002"
     Site            order(61..66,96,140..142,169..171,195,197,211,239,
                     264..266)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260002"
     Site            order(127,130..131,251..254,256,262,271,275)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:260002"
     CDS             1..327
                     /gene="LOC117611267"
                     /coded_by="XM_034339216.2:50..1033"
                     /db_xref="GeneID:117611267"
ORIGIN      
        1 mdneketkse encsspeedv fitcrgdlts yfedwdhsin kvylskipqi ckdepcqlgi
       61 deagrgpvlg pmlygisyap lsmkqllvdl gcadskslte ekrdvifdki cekrdkmgwa
      121 veaispnfia nsmyrrskts lnevsmtsai eltkkiieag awisevyvdt vgrpekyqak
      181 leqifpgiki tvakkadsty pivsaasica kvsrdhaira wqflegsiak eygsgypndp
      241 dtknwliqnv dpvfgfpqlv rfswstaeki leekalsvew eevedggnpg eqkisnfffr
      301 sppkscqskr krykffadrc lfntttl
//
LOCUS       XP_034195108             308 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611268 [Osmia lignaria lignaria].
ACCESSION   XP_034195108
VERSION     XP_034195108.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339217.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="uncharacterized protein LOC117611268"
                     /calculated_mol_wt=34357
     Region          50..133
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          154..>228
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          173..177
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409418"
     Region          187..191
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409418"
     Region          220..224
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409418"
     CDS             1..308
                     /gene="LOC117611268"
                     /coded_by="XM_034339217.2:422..1348"
                     /db_xref="GeneID:117611268"
ORIGIN      
        1 mipwsarvpp lprmrfemnq rallsrliai safmlhsgil alrmtalqip qhvvlnetvr
       61 mqcnfnldne vlysvkwykd ghefyrylpr dvpsvqtfrv tgvnvntqns teravvlnnv
      121 nlissgryrc evsaeapafq tvsdhadmtv vvlpdegpri tggrsrykvg evvrmnctsa
      181 pskpaalltw fingdpadsq ylkgphitav dekgletavl glefrvtkqh fkrgnmklkc
      241 latiatvylq sneesvkgge tipnapvmes reinpqshtr ndlmngsqta mtlswmiivl
      301 itgviltr
//
LOCUS       XP_034195110             287 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611269 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195110
VERSION     XP_034195110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="uncharacterized protein LOC117611269 isoform X1"
                     /calculated_mol_wt=32219
     Region          28..111
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          132..>173
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          153..157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409418"
     Region          167..171
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409418"
     CDS             1..287
                     /gene="LOC117611269"
                     /coded_by="XM_034339219.2:442..1305"
                     /db_xref="GeneID:117611269"
ORIGIN      
        1 mdwrvgllli cmlaedteal rmlelvvpqy vvrgqsirle cnfnldgetl ysvkwykdgn
       61 efyryvpqdr ppvlvfqlpg vtanihnste rsvvlysvnl mstgryrcev saeapsfqtv
      121 sdhsdmmvva lpedgpvitg rpgrhryqvr dvvrfnctsa kskpaamlsw fingepvdtq
      181 ylrgphitev dregletavl glefrlranh fkrgdlkikc latiatvywk snelsiegek
      241 plkmpvmesr etraqghtha ehilggsgss alvpciiplv tcllilr
//
LOCUS       XP_034195111             284 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611269 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195111
VERSION     XP_034195111.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339220.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="uncharacterized protein LOC117611269 isoform X2"
                     /calculated_mol_wt=31738
     Region          25..108
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          129..>170
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          150..154
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409418"
     Region          164..168
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409418"
     CDS             1..284
                     /gene="LOC117611269"
                     /coded_by="XM_034339220.2:269..1123"
                     /db_xref="GeneID:117611269"
ORIGIN      
        1 maypsnlvcn ngntealrml elvvpqyvvr gqsirlecnf nldgetlysv kwykdgnefy
       61 ryvpqdrppv lvfqlpgvta nihnstersv vlysvnlmst gryrcevsae apsfqtvsdh
      121 sdmmvvalpe dgpvitgrpg rhryqvrdvv rfnctsaksk paamlswfin gepvdtqylr
      181 gphitevdre gletavlgle frlranhfkr gdlkikclat iatvywksne lsiegekplk
      241 mpvmesretr aqghthaehi lggsgssalv pciiplvtcl lilr
//
LOCUS       XP_034195112             266 aa            linear   INV 03-SEP-2025
DEFINITION  cell adhesion molecule 2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195112
VERSION     XP_034195112.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339221.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..266
                     /product="cell adhesion molecule 2 isoform X3"
                     /calculated_mol_wt=29788
     Region          7..90
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          111..>152
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          132..136
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409418"
     Region          146..150
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409418"
     CDS             1..266
                     /gene="LOC117611269"
                     /coded_by="XM_034339221.2:1064..1864"
                     /db_xref="GeneID:117611269"
ORIGIN      
        1 mlelvvpqyv vrgqsirlec nfnldgetly svkwykdgne fyryvpqdrp pvlvfqlpgv
       61 tanihnster svvlysvnlm stgryrcevs aeapsfqtvs dhsdmmvval pedgpvitgr
      121 pgrhryqvrd vvrfnctsak skpaamlswf ingepvdtqy lrgphitevd regletavlg
      181 lefrlranhf krgdlkikcl atiatvywks nelsiegekp lkmpvmesre traqghthae
      241 hilggsgssa lvpciiplvt cllilr
//
LOCUS       XP_034195113             243 aa            linear   INV 03-SEP-2025
DEFINITION  gemin 2 [Osmia lignaria lignaria].
ACCESSION   XP_034195113
VERSION     XP_034195113.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339222.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="gemin 2"
                     /calculated_mol_wt=27742
     Region          20..237
                     /region_name="SIP1"
                     /note="Survival motor neuron (SMN) interacting protein 1
                     (SIP1); pfam04938"
                     /db_xref="CDD:461493"
     CDS             1..243
                     /gene="Gem2"
                     /coded_by="XM_034339222.2:106..837"
                     /db_xref="GeneID:117611270"
ORIGIN      
        1 mteyfvepaf ivgeinedin fnlppvsgee yikrvvieaq qcadvvvadi dhkqlrkpti
       61 dveplsgcve appwlgptid wqlyqvsefs narlyisqlk ndvqtfkhkw krpninlpdi
      121 gdekgwikfc sgcykekikt aptlsvifcl nqpiverile ylvehlatqk nieyqlgqwi
      181 yallvilemp ltpdvcsclr slartcsvmr aeskqlevhe igalnlficl varyfrqldl
      241 adp
//
LOCUS       XP_034195114             150 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-like protein 4A [Osmia lignaria lignaria].
ACCESSION   XP_034195114
VERSION     XP_034195114.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339223.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="ubiquitin-like protein 4A"
                     /calculated_mol_wt=16557
     Region          1..72
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          99..142
                     /region_name="Tugs"
                     /note="Tethering Ubl4a to BAGS domain; pfam17840"
                     /db_xref="CDD:465528"
     CDS             1..150
                     /gene="LOC117611271"
                     /coded_by="XM_034339223.2:90..542"
                     /db_xref="GeneID:117611271"
ORIGIN      
        1 mkvivkklqg kecvvdimps dtvlqlkhkv sdllgidvpq qrllltgktl adenplsfyp
       61 gikdgsklnl lvikkadegs gegkashsks gtnllrdevs rvlrhyytes etesivneli
      121 kdlknkvnnl syddlerlat allqdqenia
//
LOCUS       XP_076545493            1270 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545493
VERSION     XP_076545493.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689378.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X1"
                     /calculated_mol_wt=139638
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1129
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1270
                     /gene="PMCA"
                     /coded_by="XM_076689378.1:981..4793"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qlrvirafks tledleerrs vhslhslhsm rssrshtgpr
     1141 plsdftyide dptntalnag klpnksadqn llvdhsaaar mqsannltsp llltvtgsan
     1201 aynhhntint tndnrsssns alnqalspnh tqmqssnsnn kdsntgnsll lssnnlalpe
     1261 ltklvhetsi
//
LOCUS       XP_034184949            1270 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184949
VERSION     XP_034184949.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329058.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X1"
                     /calculated_mol_wt=139638
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1129
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1270
                     /gene="PMCA"
                     /coded_by="XM_034329058.2:667..4479"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qlrvirafks tledleerrs vhslhslhsm rssrshtgpr
     1141 plsdftyide dptntalnag klpnksadqn llvdhsaaar mqsannltsp llltvtgsan
     1201 aynhhntint tndnrsssns alnqalspnh tqmqssnsnn kdsntgnsll lssnnlalpe
     1261 ltklvhetsi
//
LOCUS       XP_034184950            1270 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184950
VERSION     XP_034184950.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329059.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X1"
                     /calculated_mol_wt=139638
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1129
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1270
                     /gene="PMCA"
                     /coded_by="XM_034329059.2:651..4463"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qlrvirafks tledleerrs vhslhslhsm rssrshtgpr
     1141 plsdftyide dptntalnag klpnksadqn llvdhsaaar mqsannltsp llltvtgsan
     1201 aynhhntint tndnrsssns alnqalspnh tqmqssnsnn kdsntgnsll lssnnlalpe
     1261 ltklvhetsi
//
LOCUS       XP_034184951            1270 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184951
VERSION     XP_034184951.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329060.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X1"
                     /calculated_mol_wt=139638
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1129
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1270
                     /gene="PMCA"
                     /coded_by="XM_034329060.2:553..4365"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qlrvirafks tledleerrs vhslhslhsm rssrshtgpr
     1141 plsdftyide dptntalnag klpnksadqn llvdhsaaar mqsannltsp llltvtgsan
     1201 aynhhntint tndnrsssns alnqalspnh tqmqssnsnn kdsntgnsll lssnnlalpe
     1261 ltklvhetsi
//
LOCUS       XP_034184953            1270 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184953
VERSION     XP_034184953.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329062.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X1"
                     /calculated_mol_wt=139638
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1129
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1270
                     /gene="PMCA"
                     /coded_by="XM_034329062.2:303..4115"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qlrvirafks tledleerrs vhslhslhsm rssrshtgpr
     1141 plsdftyide dptntalnag klpnksadqn llvdhsaaar mqsannltsp llltvtgsan
     1201 aynhhntint tndnrsssns alnqalspnh tqmqssnsnn kdsntgnsll lssnnlalpe
     1261 ltklvhetsi
//
LOCUS       XP_034184954            1270 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184954
VERSION     XP_034184954.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329063.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X1"
                     /calculated_mol_wt=139638
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1129
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1270
                     /gene="PMCA"
                     /coded_by="XM_034329063.2:862..4674"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qlrvirafks tledleerrs vhslhslhsm rssrshtgpr
     1141 plsdftyide dptntalnag klpnksadqn llvdhsaaar mqsannltsp llltvtgsan
     1201 aynhhntint tndnrsssns alnqalspnh tqmqssnsnn kdsntgnsll lssnnlalpe
     1261 ltklvhetsi
//
LOCUS       XP_034184955            1270 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184955
VERSION     XP_034184955.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329064.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X1"
                     /calculated_mol_wt=139638
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1129
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1270
                     /gene="PMCA"
                     /coded_by="XM_034329064.2:317..4129"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qlrvirafks tledleerrs vhslhslhsm rssrshtgpr
     1141 plsdftyide dptntalnag klpnksadqn llvdhsaaar mqsannltsp llltvtgsan
     1201 aynhhntint tndnrsssns alnqalspnh tqmqssnsnn kdsntgnsll lssnnlalpe
     1261 ltklvhetsi
//
LOCUS       XP_076545494            1258 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545494
VERSION     XP_076545494.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689379.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1258
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X2"
                     /calculated_mol_wt=138211
     Region          61..901
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(439..441,542,565..567,612,681..683,740,743,746,770,
                     773)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(831,835..836,838..839,864,867..868)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          853..1032
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1075..1117
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1258
                     /gene="PMCA"
                     /coded_by="XM_076689379.1:556..4332"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkgde aveitgnshv
      301 sggakhegge nhqapshggg gegkkeksvl qakltklaiq igyagstiav ltvvilviqf
      361 cvttfviekk pwkntyandl vrhliigvtv lvvavpeglp lavtlslays vkkmmkdnnl
      421 vrhldacetm gnataicsdk tgtlttnrmt vvqsyicekm sktipkfsdi pshvgnlivq
      481 aisinsayts rimpsqdpte lplqvgnkte callgfvval gmnyqtirdd qpeetftrvy
      541 tfnsvrksms tvvprkgggy rlftkgasei imkkcafiyg reghlekftr emqdrlvknv
      601 iepmacdglr tisvayrdfv pgkaeinqvh idnepnwdde enivnnltcl civgiedpvr
      661 pevpdairkc qkagitvrmv tgdnintars ialkcgilkp nedflilegk efnrrirdsn
      721 gevqqhlldk vwpklrvlar ssptdkytlv kgiidskatv srevvavtgd gtndgpalkk
      781 advgfamgia gtdvakeasd iiltddnfss ivkavmwgrn vydsiakflq fqltvnvvav
      841 ivafigacav qdsplkavqm lwvnlimdtl aslalatelp tsdlllrrpy grtkplisrt
      901 mmknilgqav yqltvifmll fvgdkmldie tgrgvaqagg gptqhftvif ntfvmmtlfn
      961 efnarkihgq rnvfqgiftn pifysiwvgt clsqvliiqy gkmafstkal tleqwmwclf
     1021 fgvgtllwgq iittiptrki pkilswgrgq pddigvinlg dekfdpdsdk kprtgqilwi
     1081 rgltrlqtql rvirafkstl edleerrsvh slhslhsmrs srshtgprpl sdftyidedp
     1141 tntalnagkl pnksadqnll vdhsaaarmq sannltspll ltvtgsanay nhhntinttn
     1201 dnrsssnsal nqalspnhtq mqssnsnnkd sntgnsllls snnlalpelt klvhetsi
//
LOCUS       XP_076545495            1221 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545495
VERSION     XP_076545495.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689380.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1221
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X3"
                     /calculated_mol_wt=134567
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1156
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1221
                     /gene="PMCA"
                     /coded_by="XM_076689380.1:556..4221"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qtsnrtlvqn vsvtgrspsq dyymviggel qerlipvpys
     1141 ksstdqairv vnafrqglda rytsehsstp laevlrkqss lskrlsqtss ieyadnnpde
     1201 ltipeidver lsshshteta v
//
LOCUS       XP_076545496            1198 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545496
VERSION     XP_076545496.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689381.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1198
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X4"
                     /calculated_mol_wt=132097
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..>1135
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1198
                     /gene="PMCA"
                     /coded_by="XM_076689381.1:556..4152"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qtsnrtlvqn vsvtgrspsq dyymirvvna frqgldaryt
     1141 sehsstplae vlrkqsslsk rlsqtssiey adnnpdelti peidverlss hshtetav
//
LOCUS       XP_076545497            1198 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545497
VERSION     XP_076545497.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689382.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1198
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X5"
                     /calculated_mol_wt=131981
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..1133
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1198
                     /gene="PMCA"
                     /coded_by="XM_076689382.1:556..4152"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qviggelqer lipvpyskss tdqairvvna frqgldaryt
     1141 sehsstplae vlrkqsslsk rlsqtssiey adnnpdelti peidverlss hshtetav
//
LOCUS       XP_076545498            1175 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545498
VERSION     XP_076545498.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689383.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1175
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X6"
                     /calculated_mol_wt=129511
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..>1112
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1175
                     /gene="PMCA"
                     /coded_by="XM_076689383.1:556..4083"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qirvvnafrq gldarytseh sstplaevlr kqsslskrls
     1141 qtssieyadn npdeltipei dverlsshsh tetav
//
LOCUS       XP_076545499            1126 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545499
VERSION     XP_076545499.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689384.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1126
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X7"
                     /calculated_mol_wt=123947
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     Region          1087..>1101
                     /region_name="ATP_Ca_trans_C"
                     /note="Plasma membrane calcium transporter ATPase C
                     terminal; pfam12424"
                     /db_xref="CDD:463575"
     CDS             1..1126
                     /gene="PMCA"
                     /coded_by="XM_076689384.1:556..3936"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilswgr gqpddigvin lgdekfdpds
     1081 dkkprtgqil wirgltrlqt qgvssliqkq evrmillsgs ltrcsi
//
LOCUS       XP_076545500            1059 aa            linear   INV 03-SEP-2025
DEFINITION  plasma membrane calcium-transporting ATPase 3 isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545500
VERSION     XP_076545500.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689385.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1059
                     /product="plasma membrane calcium-transporting ATPase 3
                     isoform X8"
                     /calculated_mol_wt=116400
     Region          61..913
                     /region_name="P-type_ATPase_Ca_PMCA-like"
                     /note="animal plasma membrane Ca2(+)-ATPases (PMCA),
                     similar to human ATP2B1-4/PMCA1-4, and related
                     Ca2(+)-ATPases including Saccharomyces cerevisiae vacuolar
                     PMC1; cd02081"
                     /db_xref="CDD:319776"
     Site            order(451..453,554,577..579,624,693..695,752,755,758,782,
                     785)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319776"
     Site            order(843,847..848,850..851,876,879..880)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319776"
     Region          865..1044
                     /region_name="Cation_ATPase_C"
                     /note="Cation transporting ATPase, C-terminus; pfam00689"
                     /db_xref="CDD:376368"
     CDS             1..1059
                     /gene="PMCA"
                     /coded_by="XM_076689385.1:556..3735"
                     /db_xref="GeneID:117606523"
ORIGIN      
        1 matidgrpaq ygitlkqlre lmelrgregv nkinsyggvq eickklytsp neglsgsaad
       61 iqhrrdtfgs nmippkppkt flqlvwealq dvtliileva alvslglsfy hpsdeeddee
      121 hvsidedeak ygwieglail isvivvvlvt afndyskerq frglqsrieg ehkfsvirqg
      181 evkqisvsdi avgdicqiky gdllpadgil iqsndlkvde ssltgesdhv kkgesfdpmv
      241 lsgthvmegs gkmlvtavgv nsqagiiftl lgaavdqqeq eikkmkkeak kqrkkksltg
      301 deaveitgns hvsggakheg genhqapshg gggegkkeks vlqakltkla iqigyagsti
      361 avltvvilvi qfcvttfvie kkpwkntyan dlvrhliigv tvlvvavpeg lplavtlsla
      421 ysvkkmmkdn nlvrhldace tmgnataics dktgtlttnr mtvvqsyice kmsktipkfs
      481 dipshvgnli vqaisinsay tsrimpsqdp telplqvgnk tecallgfvv algmnyqtir
      541 ddqpeetftr vytfnsvrks mstvvprkgg gyrlftkgas eiimkkcafi ygreghlekf
      601 tremqdrlvk nviepmacdg lrtisvayrd fvpgkaeinq vhidnepnwd deenivnnlt
      661 clcivgiedp vrpevpdair kcqkagitvr mvtgdninta rsialkcgil kpnedflile
      721 gkefnrrird sngevqqhll dkvwpklrvl arssptdkyt lvkgiidska tvsrevvavt
      781 gdgtndgpal kkadvgfamg iagtdvakea sdiiltddnf ssivkavmwg rnvydsiakf
      841 lqfqltvnvv avivafigac avqdsplkav qmlwvnlimd tlaslalate lptsdlllrr
      901 pygrtkplis rtmmknilgq avyqltvifm llfvgdkmld ietgrgvaqa gggptqhftv
      961 ifntfvmmtl fnefnarkih gqrnvfqgif tnpifysiwv gtclsqvlii qygkmafstk
     1021 altleqwmwc lffgvgtllw gqiittiptr kipkilssa
//
LOCUS       XP_034185668             945 aa            linear   INV 03-SEP-2025
DEFINITION  protein meiotic P26 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185668
VERSION     XP_034185668.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329777.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..945
                     /product="protein meiotic P26 isoform X1"
                     /calculated_mol_wt=102565
     Region          68..124
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(70,73,85,87,90,93,113,116)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438226"
     Region          152..194
                     /region_name="Bbox1_BRAT-like"
                     /note="B-box-type 1 zinc finger found in Drosophila
                     melanogaster brain tumor protein (BRAT) and similar
                     proteins; cd19813"
                     /db_xref="CDD:380871"
     Region          207..250
                     /region_name="Bbox2_BRAT-like"
                     /note="B-box-type 2 zinc finger found in Drosophila
                     melanogaster brain tumor protein (BRAT) and similar
                     proteins; cd19798"
                     /db_xref="CDD:380856"
     Region          257..378
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cd20482"
                     /db_xref="CDD:467844"
     Site            order(261,264..265,267..269,272,275,289..290,293,296..297,
                     300..301,304,311..312,315,318..319,322..323,326,336..337,
                     340,344,347..348,351..352,354..355,363,366..367,370,
                     373..374)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467844"
     Region          669..942
                     /region_name="NHL_brat_like"
                     /note="NHL repeat domain of the Drosophila brain-tumor
                     protein (brat) and similar proteins; cd14959"
                     /db_xref="CDD:271329"
     Site            order(683..689,711,730..733,738,756)
                     /site_type="other"
                     /note="putative Pumilio binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271329"
     Region          692..728
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          739..781
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          784..820
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          824..863
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          868..907
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          913..939
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     CDS             1..945
                     /gene="LOC117606823"
                     /coded_by="XM_034329777.2:421..3258"
                     /db_xref="GeneID:117606823"
ORIGIN      
        1 massmvavps gaasvsaagt gitqneeveh gdvfsernin svngcnetsn pgdaleeinc
       61 gtgesgdqec aicmsklcsp rvlsclhvfc eacldkllmd eagdskvssv licplcqqet
      121 sisskgaasl tcdyvltnil dmsaienmav lctsckakes avarcsdcan flcpncntah
      181 qfmrcfeshk vvafedlkqs neaipihkpi fceyhpaenm kfycytcqep icnecllveh
      241 kapdhqyerl vdaeprqkee liklmneska riadcdqmsa qlenalselq aqhdqakdli
      301 vetfqsykav lekcrdnalv eleklhsdre leimdtfhsv ektvekieda crftsrlleh
      361 gdaveilalr rivgtqlmnl ikntpkpnvs ssiefqtdyi qfektvkdvf gkfhtertpv
      421 tkstpepist vpgvsttqqi vhtsmgcprs istrspislp asmqssfegd psfvipptss
      481 pqnipppppp vslppgpihg ltsiqeynlq qlaslaekve mvgesnvvgp ssnpsptppf
      541 tladlfagdl sstshainnl qalaklgntl gnqdlgnati nggsglvlgr gpspaivdtp
      601 nlgltansll nggfqslsss tspilsqgad dligdsmhnm pvvvggntvg nvtgsgsgny
      661 ahprsnntkl tpmhirckfg qlgpskgqfs sphgfclsad ediivadtnn hriqifektg
      721 ifkfqfgvpg keegqlwypr kvavmrnsgk fvvcdrgner srmqiftkng hfmkkiairy
      781 idivaglavt seghilavds vsptvfvisd tgdllrwfdc seymrepsdi aisgkeyfvc
      841 dfkghcvvvf neegkflrri gcdnvtnfpn gidisdagdi ligdshgnrf hvavfsrdgs
      901 lisefecpyv kvsrccglki tsegyivtla knnhhvlvln tlyis
//
LOCUS       XP_034185669             945 aa            linear   INV 03-SEP-2025
DEFINITION  protein meiotic P26 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185669
VERSION     XP_034185669.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329778.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..945
                     /product="protein meiotic P26 isoform X1"
                     /calculated_mol_wt=102565
     Region          68..124
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(70,73,85,87,90,93,113,116)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438226"
     Region          152..194
                     /region_name="Bbox1_BRAT-like"
                     /note="B-box-type 1 zinc finger found in Drosophila
                     melanogaster brain tumor protein (BRAT) and similar
                     proteins; cd19813"
                     /db_xref="CDD:380871"
     Region          207..250
                     /region_name="Bbox2_BRAT-like"
                     /note="B-box-type 2 zinc finger found in Drosophila
                     melanogaster brain tumor protein (BRAT) and similar
                     proteins; cd19798"
                     /db_xref="CDD:380856"
     Region          257..378
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cd20482"
                     /db_xref="CDD:467844"
     Site            order(261,264..265,267..269,272,275,289..290,293,296..297,
                     300..301,304,311..312,315,318..319,322..323,326,336..337,
                     340,344,347..348,351..352,354..355,363,366..367,370,
                     373..374)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467844"
     Region          669..942
                     /region_name="NHL_brat_like"
                     /note="NHL repeat domain of the Drosophila brain-tumor
                     protein (brat) and similar proteins; cd14959"
                     /db_xref="CDD:271329"
     Site            order(683..689,711,730..733,738,756)
                     /site_type="other"
                     /note="putative Pumilio binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271329"
     Region          692..728
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          739..781
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          784..820
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          824..863
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          868..907
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          913..939
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     CDS             1..945
                     /gene="LOC117606823"
                     /coded_by="XM_034329778.2:842..3679"
                     /db_xref="GeneID:117606823"
ORIGIN      
        1 massmvavps gaasvsaagt gitqneeveh gdvfsernin svngcnetsn pgdaleeinc
       61 gtgesgdqec aicmsklcsp rvlsclhvfc eacldkllmd eagdskvssv licplcqqet
      121 sisskgaasl tcdyvltnil dmsaienmav lctsckakes avarcsdcan flcpncntah
      181 qfmrcfeshk vvafedlkqs neaipihkpi fceyhpaenm kfycytcqep icnecllveh
      241 kapdhqyerl vdaeprqkee liklmneska riadcdqmsa qlenalselq aqhdqakdli
      301 vetfqsykav lekcrdnalv eleklhsdre leimdtfhsv ektvekieda crftsrlleh
      361 gdaveilalr rivgtqlmnl ikntpkpnvs ssiefqtdyi qfektvkdvf gkfhtertpv
      421 tkstpepist vpgvsttqqi vhtsmgcprs istrspislp asmqssfegd psfvipptss
      481 pqnipppppp vslppgpihg ltsiqeynlq qlaslaekve mvgesnvvgp ssnpsptppf
      541 tladlfagdl sstshainnl qalaklgntl gnqdlgnati nggsglvlgr gpspaivdtp
      601 nlgltansll nggfqslsss tspilsqgad dligdsmhnm pvvvggntvg nvtgsgsgny
      661 ahprsnntkl tpmhirckfg qlgpskgqfs sphgfclsad ediivadtnn hriqifektg
      721 ifkfqfgvpg keegqlwypr kvavmrnsgk fvvcdrgner srmqiftkng hfmkkiairy
      781 idivaglavt seghilavds vsptvfvisd tgdllrwfdc seymrepsdi aisgkeyfvc
      841 dfkghcvvvf neegkflrri gcdnvtnfpn gidisdagdi ligdshgnrf hvavfsrdgs
      901 lisefecpyv kvsrccglki tsegyivtla knnhhvlvln tlyis
//
LOCUS       XP_034185670             918 aa            linear   INV 03-SEP-2025
DEFINITION  protein meiotic P26 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185670
VERSION     XP_034185670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329779.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..918
                     /product="protein meiotic P26 isoform X2"
                     /calculated_mol_wt=99851
     Region          68..124
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(70,73,85,87,90,93,113,116)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438226"
     Region          152..194
                     /region_name="Bbox1_BRAT-like"
                     /note="B-box-type 1 zinc finger found in Drosophila
                     melanogaster brain tumor protein (BRAT) and similar
                     proteins; cd19813"
                     /db_xref="CDD:380871"
     Region          207..250
                     /region_name="Bbox2_BRAT-like"
                     /note="B-box-type 2 zinc finger found in Drosophila
                     melanogaster brain tumor protein (BRAT) and similar
                     proteins; cd19798"
                     /db_xref="CDD:380856"
     Region          257..378
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cd20482"
                     /db_xref="CDD:467844"
     Site            order(261,264..265,267..269,272,275,289..290,293,296..297,
                     300..301,304,311..312,315,318..319,322..323,326,336..337,
                     340,344,347..348,351..352,354..355,363,366..367,370,
                     373..374)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467844"
     Region          642..915
                     /region_name="NHL_brat_like"
                     /note="NHL repeat domain of the Drosophila brain-tumor
                     protein (brat) and similar proteins; cd14959"
                     /db_xref="CDD:271329"
     Site            order(656..662,684,703..706,711,729)
                     /site_type="other"
                     /note="putative Pumilio binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271329"
     Region          665..701
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          712..754
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          757..793
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          797..836
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          841..880
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     Region          886..912
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271329"
     CDS             1..918
                     /gene="LOC117606823"
                     /coded_by="XM_034329779.2:421..3177"
                     /db_xref="GeneID:117606823"
ORIGIN      
        1 massmvavps gaasvsaagt gitqneeveh gdvfsernin svngcnetsn pgdaleeinc
       61 gtgesgdqec aicmsklcsp rvlsclhvfc eacldkllmd eagdskvssv licplcqqet
      121 sisskgaasl tcdyvltnil dmsaienmav lctsckakes avarcsdcan flcpncntah
      181 qfmrcfeshk vvafedlkqs neaipihkpi fceyhpaenm kfycytcqep icnecllveh
      241 kapdhqyerl vdaeprqkee liklmneska riadcdqmsa qlenalselq aqhdqakdli
      301 vetfqsykav lekcrdnalv eleklhsdre leimdtfhsv ektvekieda crftsrlleh
      361 gdaveilalr rivgtqlmnl ikntpkpnvs ssiefqtdyi qfektvkdvf gkfhtertpv
      421 tkstpepist vpgvsttqqi vhtsmgcprs istrspislp asmqssfegp ihgltsiqey
      481 nlqqlaslae kvemvgesnv vgpssnpspt ppftladlfa gdlsstshai nnlqalaklg
      541 ntlgnqdlgn atinggsglv lgrgpspaiv dtpnlgltan sllnggfqsl ssstspilsq
      601 gaddligdsm hnmpvvvggn tvgnvtgsgs gnyahprsnn tkltpmhirc kfgqlgpskg
      661 qfssphgfcl sadediivad tnnhriqife ktgifkfqfg vpgkeegqlw yprkvavmrn
      721 sgkfvvcdrg nersrmqift knghfmkkia iryidivagl avtseghila vdsvsptvfv
      781 isdtgdllrw fdcseymrep sdiaisgkey fvcdfkghcv vvfneegkfl rrigcdnvtn
      841 fpngidisda gdiligdshg nrfhvavfsr dgslisefec pyvkvsrccg lkitsegyiv
      901 tlaknnhhvl vlntlyis
//
LOCUS       XP_034184963             708 aa            linear   INV 03-SEP-2025
DEFINITION  myotubularin-related protein 10-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184963
VERSION     XP_034184963.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329072.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="myotubularin-related protein 10-B isoform X1"
                     /calculated_mol_wt=81208
     Region          89..238
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          313..508
                     /region_name="PTP-MTMR10-like"
                     /note="protein tyrosine phosphatase-like pseudophosphatase
                     domain of myotubularin related phosphoinositide
                     phosphatases 10, 11, and 12; cd14537"
                     /db_xref="CDD:350385"
     Region          <554..>626
                     /region_name="3-PAP"
                     /note="Myotubularin-associated protein; pfam12578"
                     /db_xref="CDD:463634"
     CDS             1..708
                     /gene="LOC117606524"
                     /coded_by="XM_034329072.2:193..2319"
                     /db_xref="GeneID:117606524"
ORIGIN      
        1 mqshysnysn vrnmktanee envkklcrmi raggytvisf wmrnsmqsta ivetfamesk
       61 nynnfvsyvg leehemqplg ssrrnslses nikllpgeil vtnaqnvlmf spvsdlkqgt
      121 sgilsvtnfk ltfitsedtn gedtsrqqnh lygymdtcli niediyitvg dkkrklvpgn
      181 tvpskikgif ivcknfrtws fsfkfspsgq vkklltalll hafpsrhqll faydyqeayy
      241 ssldkavrlf rdisdwhnel erticneklr kywrlstvnv dfklcrslsr yiivpasitd
      301 nqlldaakhf qgnrppiwsw ssahgaalvk mselsplitn rmqenimfen vrkshpqkmp
      361 pivlelnkei siklvavafs klanlcspen irqfwlqdnn fyslventkw fkyvsyclqk
      421 tveacehlhl gfsvilqega gtdiccviss lvqllldpyf rtingfqsll qkewvagghp
      481 fcdrlghivk rnseksplfl lfldcvwqls qqfpaefeft etylttlwda ahvsifdtfi
      541 fncekdrvaa atepnnplvl rsvwdwreqf sdqdillfyn plynsreads tdkciikpwy
      601 niaslelwtq cyfrwiptle irnggqnhie lyarllrndv nqlkisidgn cgspiaksni
      661 ysvqmnidsf ypfsnkksgn aistpimnss ilvtesllda qslitasd
//
LOCUS       XP_034184964             707 aa            linear   INV 03-SEP-2025
DEFINITION  myotubularin-related protein 10-B isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184964
VERSION     XP_034184964.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329073.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..707
                     /product="myotubularin-related protein 10-B isoform X2"
                     /calculated_mol_wt=81079
     Region          89..238
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          313..507
                     /region_name="PTP-MTMR10-like"
                     /note="protein tyrosine phosphatase-like pseudophosphatase
                     domain of myotubularin related phosphoinositide
                     phosphatases 10, 11, and 12; cd14537"
                     /db_xref="CDD:350385"
     Region          <553..>625
                     /region_name="3-PAP"
                     /note="Myotubularin-associated protein; pfam12578"
                     /db_xref="CDD:463634"
     CDS             1..707
                     /gene="LOC117606524"
                     /coded_by="XM_034329073.2:196..2319"
                     /db_xref="GeneID:117606524"
ORIGIN      
        1 mqshysnysn vrnmktanee envkklcrmi raggytvisf wmrnsmqsta ivetfamesk
       61 nynnfvsyvg leehemqplg ssrrnslses nikllpgeil vtnaqnvlmf spvsdlkqgt
      121 sgilsvtnfk ltfitsedtn gedtsrqqnh lygymdtcli niediyitvg dkkrklvpgn
      181 tvpskikgif ivcknfrtws fsfkfspsgq vkklltalll hafpsrhqll faydyqeayy
      241 ssldkavrlf rdisdwhnel erticneklr kywrlstvnv dfklcrslsr yiivpasitd
      301 nqlldaakhf qgnrppiwsw ssahgaalvk mselsplitn rmqenimfen vrkshpqkmp
      361 pivlelnkei siklvavafs klanlcspen irqfwlqdnn fyslventkw fkyvsyclqk
      421 tveacehlhl gfsvilqgag tdiccvissl vqllldpyfr tingfqsllq kewvagghpf
      481 cdrlghivkr nseksplfll fldcvwqlsq qfpaefefte tylttlwdaa hvsifdtfif
      541 ncekdrvaaa tepnnplvlr svwdwreqfs dqdillfynp lynsreadst dkciikpwyn
      601 iaslelwtqc yfrwiptlei rnggqnhiel yarllrndvn qlkisidgnc gspiaksniy
      661 svqmnidsfy pfsnkksgna istpimnssi lvteslldaq slitasd
//
LOCUS       XP_076545501             541 aa            linear   INV 03-SEP-2025
DEFINITION  CXXC-type zinc finger protein 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545501
VERSION     XP_076545501.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689386.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..541
                     /product="CXXC-type zinc finger protein 1 isoform X1"
                     /calculated_mol_wt=63717
     Region          38..80
                     /region_name="PHD_Cfp1"
                     /note="PHD finger found in CXXC-type zinc finger protein 1
                     (Cfp1); cd15553"
                     /db_xref="CDD:277028"
     Site            order(38,49..53,57,78)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277028"
     Region          151..198
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          264..497
                     /region_name="CpG_bind_C"
                     /note="CpG binding protein C-terminal domain; pfam12269"
                     /db_xref="CDD:463514"
     CDS             1..541
                     /gene="LOC117606525"
                     /coded_by="XM_076689386.1:111..1736"
                     /db_xref="GeneID:117606525"
ORIGIN      
        1 madkrqqnls keeiakqfml perkskiatl lkqdgqayci crssdssrfm igcdaceewy
       61 hgdcinitek dakhikqffc ivrtllvnvn dfyltnyink iylqrcreed ptlitrykpr
      121 ktdqedrkyk khkekerahr yeydapwdpt vikksskrcg ectgclrten cgkcdacrhl
      181 kkfgpsvrlk lrciqrtcrv lgdplkpsks fnksskfikk rkrdssnern dyldvprhcy
      241 gpactkcsrp gskycsdecg lklatnriyq vlpqrlqews ltpciaeqnn rrqleairkq
      301 qqdvrrilqe ldkrhaeldr iierakhati dpqaevddnd dtemsmycit cgheihsrts
      361 ikhmekcfnk yesqasfgsi fktriegqvm fcdfynpanr tyckrlrvlc pehckdpkis
      421 etevcgcplv tnvfdttgef crapkkscvk hyvweklrra eidmervrqw lkidelveqe
      481 rqiranmatr agvlalmlhs tynhelmeqm tqeqnreqle ameeefqrry gllqkeqtae
      541 q
//
LOCUS       XP_034184966             518 aa            linear   INV 03-SEP-2025
DEFINITION  CXXC-type zinc finger protein 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184966
VERSION     XP_034184966.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329075.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..518
                     /product="CXXC-type zinc finger protein 1 isoform X2"
                     /calculated_mol_wt=60918
     Region          38..83
                     /region_name="PHD_Cfp1"
                     /note="PHD finger found in CXXC-type zinc finger protein 1
                     (Cfp1); cd15553"
                     /db_xref="CDD:277028"
     Site            order(38,49..53,57,78)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277028"
     Region          128..175
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          241..474
                     /region_name="CpG_bind_C"
                     /note="CpG binding protein C-terminal domain; pfam12269"
                     /db_xref="CDD:463514"
     CDS             1..518
                     /gene="LOC117606525"
                     /coded_by="XM_034329075.2:111..1667"
                     /db_xref="GeneID:117606525"
ORIGIN      
        1 madkrqqnls keeiakqfml perkskiatl lkqdgqayci crssdssrfm igcdaceewy
       61 hgdcinitek dakhikqffc ircreedptl itrykprktd qedrkykkhk ekerahryey
      121 dapwdptvik ksskrcgect gclrtencgk cdacrhlkkf gpsvrlklrc iqrtcrvlgd
      181 plkpsksfnk sskfikkrkr dssnerndyl dvprhcygpa ctkcsrpgsk ycsdecglkl
      241 atnriyqvlp qrlqewsltp ciaeqnnrrq leairkqqqd vrrilqeldk rhaeldriie
      301 rakhatidpq aevddnddte msmycitcgh eihsrtsikh mekcfnkyes qasfgsifkt
      361 riegqvmfcd fynpanrtyc krlrvlcpeh ckdpkisete vcgcplvtnv fdttgefcra
      421 pkkscvkhyv weklrraeid mervrqwlki delveqerqi ranmatragv lalmlhstyn
      481 helmeqmtqe qnreqleame eefqrrygll qkeqtaeq
//
LOCUS       XP_034185671             521 aa            linear   INV 03-SEP-2025
DEFINITION  ric8 guanine nucleotide exchange factor A [Osmia lignaria
            lignaria].
ACCESSION   XP_034185671
VERSION     XP_034185671.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185671.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..521
                     /product="ric8 guanine nucleotide exchange factor A"
                     /calculated_mol_wt=59456
     Region          65..481
                     /region_name="Ric8"
                     /note="Guanine nucleotide exchange factor synembryn;
                     pfam10165"
                     /db_xref="CDD:462973"
     CDS             1..521
                     /gene="ric8a"
                     /coded_by="XM_034329780.2:66..1631"
                     /db_xref="GeneID:117606824"
ORIGIN      
        1 mdslvkeiis dtadkfandv vtfqnnygtr ttfeelnkdh lreklwetlf sylsnssess
       61 vhhnclsmlr ilsrdktdln ylitnerini ilenaalkdt nvdqvsynnv iiealkllcn
      121 lifnstkvlq ilpntsclpc liklmikydn dipyevmlfn iriiflital nistrqfvkt
      181 vlngneclin ilenikirse qkntieadda tlaceilkal fnlyiqsddt tqeketdesl
      241 vsilyklllf qcekendlqs nianlltvip ydcystiipr cnekckhvyq dvdmsaisvl
      301 lqfldrrlnc kedlignlsp ivtafirlik aerlirkytr lkilpplkdv mhrpeegttl
      361 raklckllts pltelrdlva eflfilcken vvrmvkytgy gnaagmfank gllgpnkgks
      421 ayssesedse teeylkhkeq inpvigcfeq pkpnplegmt eeqkeyealk lvglvdkltr
      481 eglvqpcrig ddgkpkaieh vlelqeelpk qqygrqdsds d
//
LOCUS       XP_034185673             496 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606825 [Osmia lignaria lignaria].
ACCESSION   XP_034185673
VERSION     XP_034185673.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329782.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="uncharacterized protein LOC117606825"
                     /calculated_mol_wt=54267
     Region          32..116
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          338..382
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..496
                     /gene="LOC117606825"
                     /coded_by="XM_034329782.2:4198..5688"
                     /db_xref="GeneID:117606825"
ORIGIN      
        1 mastsqqycl rwnnhrsnll tvfdellqne sftdvtlavd ggasvkchkm vlaacssyfq
       61 tlfidlpckh pivvlkdvky seikaileym yrgevnvaqe qlagllkvae vlkvkglvee
      121 ngsqsrreev etsmspppai ttsttssaah ssglaspphs tgtsyvygrs pvdrtnqmpl
      181 pmwplsglpi shsssnyqtt shpqhsstlt gsydngfets plkrkklsni lmnrdtpilr
      241 tvlgqghads sqgipllhpd shenhfrnns ngssndndrr sstdiphgep ahspytdvsm
      301 mdeddkqpsp qsyppdkpgi vnyvpaqkpe wkrykqytrk dimsaieavr tgmsalqaar
      361 kygvpsrtly dkvkklgitt srpfkrgsng sgacfpygig gnanggiysg alsenenens
      421 saidspgsil daykardgpm drdmldvtrc sstpiihsvk qqsnedqved lsvtrksdvr
      481 vivpptsvvk needvd
//
LOCUS       XP_034185684             496 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606825 [Osmia lignaria lignaria].
ACCESSION   XP_034185684
VERSION     XP_034185684.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329793.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="uncharacterized protein LOC117606825"
                     /calculated_mol_wt=54267
     Region          32..116
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          338..382
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..496
                     /gene="LOC117606825"
                     /coded_by="XM_034329793.2:2245..3735"
                     /db_xref="GeneID:117606825"
ORIGIN      
        1 mastsqqycl rwnnhrsnll tvfdellqne sftdvtlavd ggasvkchkm vlaacssyfq
       61 tlfidlpckh pivvlkdvky seikaileym yrgevnvaqe qlagllkvae vlkvkglvee
      121 ngsqsrreev etsmspppai ttsttssaah ssglaspphs tgtsyvygrs pvdrtnqmpl
      181 pmwplsglpi shsssnyqtt shpqhsstlt gsydngfets plkrkklsni lmnrdtpilr
      241 tvlgqghads sqgipllhpd shenhfrnns ngssndndrr sstdiphgep ahspytdvsm
      301 mdeddkqpsp qsyppdkpgi vnyvpaqkpe wkrykqytrk dimsaieavr tgmsalqaar
      361 kygvpsrtly dkvkklgitt srpfkrgsng sgacfpygig gnanggiysg alsenenens
      421 saidspgsil daykardgpm drdmldvtrc sstpiihsvk qqsnedqved lsvtrksdvr
      481 vivpptsvvk needvd
//
LOCUS       XP_034184967             477 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-1,3-mannosyl-glycoprotein
            2-beta-N-acetylglucosaminyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034184967
VERSION     XP_034184967.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329076.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="alpha-1,3-mannosyl-glycoprotein
                     2-beta-N-acetylglucosaminyltransferase"
                     /calculated_mol_wt=55458
     Region          4..>77
                     /region_name="PilN"
                     /note="Type IV pilus assembly protein PilN [Cell motility,
                     Extracellular structures]; COG3166"
                     /db_xref="CDD:442399"
     Region          138..465
                     /region_name="GT13_GLCNAC-TI"
                     /note="GT13_GLCNAC-TI is involved in an essential step in
                     the synthesis of complex or hybrid-type N-linked
                     oligosaccharides; cd02514"
                     /db_xref="CDD:133007"
     Site            order(143..145,147,174..175,216..217,220..221,241..243,
                     299,320..321,350,352)
                     /site_type="active"
                     /note="substrate binding site [active]"
                     /db_xref="CDD:133007"
     CDS             1..477
                     /gene="Mgat1"
                     /coded_by="XM_034329076.2:18..1451"
                     /db_xref="GeneID:117606526"
ORIGIN      
        1 mrnrsvsiif gslvlwglit tyflisdqpv nkeqdtarvs nqisklerri kqeiilnqel
       61 lqdikedkqr kkkldtvntn pnekqkdany nsekvspeke ekeegnvdkn aqplytepne
      121 isrnvptkgk lpngspiiav lvfscnrvtv krcldqliky rpsveqfpii vsedcqhrqt
      181 aeiinrygnq imhiqqpdqs dievppkekk fkgyfkiarh ygwalnyvff qlgydtviiv
      241 eddldvapdf feyflgtypl lvsdntlwcv sawndngkag lvdengsdil yrtdffpglg
      301 wmltrqlwae lspkwpksyw ddwirqpeqr rnracirpei srtrtfgktg vsngmfyerh
      361 lkyiklntef vhftkmnlty llkdnydisf vndvyqstvv sfselkngkv mapgavripy
      421 ysrqayknta kqfglmddfr sgvprtgyrg vvtffykgrr vhlapsanwn gyditws
//
LOCUS       XP_034184971             459 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606528 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184971
VERSION     XP_034184971.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329080.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="uncharacterized protein LOC117606528 isoform X1"
                     /calculated_mol_wt=51575
     Region          45..400
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          123..183
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          125..148
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          149..172
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          157..264
                     /region_name="LRR_5"
                     /note="BspA type Leucine rich repeat region (6 copies);
                     pfam13306"
                     /db_xref="CDD:463839"
     Region          173..196
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          197..220
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          221..244
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          245..267
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          268..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          292..315
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..459
                     /gene="LOC117606528"
                     /coded_by="XM_034329080.2:31..1410"
                     /db_xref="GeneID:117606528"
ORIGIN      
        1 mrpriakflr svsfsqksen lyllqslsfc wyghagtsaa grnvfldaea calktlglmy
       61 craqiciyyl lwtatlsprl tleqetstak nwdcpaecic lspkqvlcnt gglkdiptqq
      121 lsptveelsl tknnfpiikg dafaglrvlr kltmdgnnis tirpfafrgl srlrelsiqy
      181 tplpfiekfs faalqnvsvl llannkiryi egysfagtsn irvillsnnp llriqshafs
      241 gltnvvrlif psgirtiepd afdglqhvgl lkltymdlss lqsytfrglf yvhslniqes
      301 dlgiiekdaf tglsrvdrln ilnnkidlie rlflkyenni ellrfhgnhv leaprhakdv
      361 nlevasisai dnhfpcdcqa hnilesdfvn gtvhefqkkn ycispieyng kpmnlvdfdl
      421 iarchdnvvq dnlgsgvvgi ftlpttlfli llfsmnlfq
//
LOCUS       XP_034184972             401 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606528 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184972
VERSION     XP_034184972.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329081.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..401
                     /product="uncharacterized protein LOC117606528 isoform X2"
                     /calculated_mol_wt=45167
     Region          17..342
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..125
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          67..90
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          91..114
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          99..206
                     /region_name="LRR_5"
                     /note="BspA type Leucine rich repeat region (6 copies);
                     pfam13306"
                     /db_xref="CDD:463839"
     Region          115..138
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          139..162
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          163..186
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          187..209
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          210..233
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          234..257
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..401
                     /gene="LOC117606528"
                     /coded_by="XM_034329081.2:219..1424"
                     /db_xref="GeneID:117606528"
ORIGIN      
        1 mycraqiciy yllwtatlsp rltleqetst aknwdcpaec iclspkqvlc ntgglkdipt
       61 qqlsptveel sltknnfpii kgdafaglrv lrkltmdgnn istirpfafr glsrlrelsi
      121 qytplpfiek fsfaalqnvs vlllannkir yiegysfagt snirvillsn npllriqsha
      181 fsgltnvvrl ifpsgirtie pdafdglqhv gllkltymdl sslqsytfrg lfyvhslniq
      241 esdlgiiekd aftglsrvdr lnilnnkidl ierlflkyen niellrfhgn hvleaprhak
      301 dvnlevasis aidnhfpcdc qahnilesdf vngtvhefqk knycispiey ngkpmnlvdf
      361 dliarchdnv vqdnlgsgvv giftlpttlf lillfsmnlf q
//
LOCUS       XP_034184973             400 aa            linear   INV 03-SEP-2025
DEFINITION  NADH:ubiquinone oxidoreductase subunit 39 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184973
VERSION     XP_034184973.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329082.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="NADH:ubiquinone oxidoreductase subunit 39"
                     /calculated_mol_wt=45378
     Region          57..332
                     /region_name="NDUFA9_like_SDR_a"
                     /note="NADH dehydrogenase (ubiquinone) 1 alpha subcomplex,
                     subunit 9, 39 kDa, (NDUFA9) -like, atypical (a) SDRs;
                     cd05271"
                     /db_xref="CDD:187579"
     Site            order(63,65..68,87..89,132..134,147,170..172,190,194,
                     213..216)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187579"
     Site            order(190,194)
                     /site_type="active"
                     /db_xref="CDD:187579"
     CDS             1..400
                     /gene="ND-39"
                     /coded_by="XM_034329082.1:86..1288"
                     /db_xref="GeneID:117606529"
ORIGIN      
        1 maawlktaiq aaksqnvyaa syaiqsrdcs skpyiiknpt taslkrgtgg rssfngvvct
       61 ifgcsgfigs yvcnrlgkig tqlilpyrgd hyhvrplkvc gdlgqvlfhp fhlkdeesil
      121 ksmkysnvvi nlissyypts nfsyhdvnvk garriarlak ecgverfihv sclnasekpk
      181 plmikngsei lktkwegeca vkeefpeati irpatiygqe dnfithymna wrrnlktvpl
      241 weggektekq pvhisdvaag itavatnplt agktyefigp rryklialvk wfyeiasrgq
      301 gdyykiidmk ynplfrlkie lnefihrlhr tqpmnweile inhtsdninp gvptledlgi
      361 tpctmesrmq welmpyrfen hfietvdeqk asdpetipik
//
LOCUS       XP_034184975             173 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184975
VERSION     XP_034184975.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329084.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..173
                     /product="NADH dehydrogenase [ubiquinone] 1 alpha
                     subcomplex subunit 8"
                     /calculated_mol_wt=20047
     CDS             1..173
                     /gene="ND-19"
                     /coded_by="XM_034329084.2:6..527"
                     /db_xref="GeneID:117606530"
ORIGIN      
        1 mvatkdtvfp sdeeltvqei nvswpglqaa svyigkkcew hnnefmlckk elhdprqcin
       61 egknvtscam evfrgikkhc knefnnyshc lerssssmel drcrntqkvf dncalknlni
      121 erpsfgyfce akvhdsprpe pvpeqtvypd atpkmirrpy rppnygyrty wsn
//
LOCUS       XP_076545502             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606532 [Osmia lignaria lignaria].
ACCESSION   XP_076545502
VERSION     XP_076545502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689387.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117606532"
                     /calculated_mol_wt=13305
     CDS             1..116
                     /gene="LOC117606532"
                     /coded_by="XM_076689387.1:426..776"
                     /db_xref="GeneID:117606532"
ORIGIN      
        1 mapylkyflq vntkiieiyn sptsppqtil amsrnttctr rwpnlprdfv pppglvawit
       61 aqkkrnrrml qthdhrfrcn tndrqkagti ivsktttake kknnsthihl asygel
//
LOCUS       XP_034185614             354 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha o subunit isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185614
VERSION     XP_034185614.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329723.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..354
                     /product="G protein alpha o subunit isoform X1"
                     /calculated_mol_wt=40273
     Region          34..348
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,182,201,204,270..271,273,325..326)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,150,179,204,212,
                     216)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            177..185
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            182
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(183,185,187,198,200,204..206,208,211..212,214..217)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(185,210,213..214,217)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            201..204
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            202..218
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            270..273
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(317..328,330..333)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            325..327
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..354
                     /gene="Galphao"
                     /coded_by="XM_034329723.2:1070..2134"
                     /db_xref="GeneID:117606804"
ORIGIN      
        1 mgcamsaeer aalarskqie knlkedgiqa akdikllllg agesgkstiv kqmkiihesg
       61 ftpedfkqyr pvvysntiqs lvailrampt lgisfstner etdakmvfdv iqrmedtepf
      121 seellaamkr lwadngvqec fgrsneyqln dsakyflddl drlgardyqp teqdilrtrv
      181 kttgivevhf sfknlnfklf dvggqrserk kwihcfedvt aiifcvamse ydqvlhedet
      241 tnrmqeslkl fdsicnnkwf tdtsiilfln kkdlfeekir ksplticfpe yagaqeygea
      301 aayiqaqfea knksttkeiy chmtcatdtn niqfvfdavt dviiannlrg cgly
//
LOCUS       XP_034185615             302 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha o subunit isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185615
VERSION     XP_034185615.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329724.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..302
                     /product="G protein alpha o subunit isoform X2"
                     /calculated_mol_wt=34790
     Region          1..296
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(19,23,27,30,33..34,52,55,98,127,152,160,164)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            125..133
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            130
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(131,133,135,146,148,152..154,156,159..160,162..165)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(133,158,161..162,165)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            149..152
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            150..166
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            218..221
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(265..276,278..281)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            273..275
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..302
                     /gene="Galphao"
                     /coded_by="XM_034329724.2:697..1605"
                     /db_xref="GeneID:117606804"
ORIGIN      
        1 mkiihesgft pedfkqyrpv vysntiqslv ailramptlg isfstneret dakmvfdviq
       61 rmedtepfse ellaamkrlw adngvqecfg rsneyqlnds akyflddldr lgardyqpte
      121 qdilrtrvkt tgivevhfsf knlnfklfdv ggqrserkkw ihcfedvtai ifcvamseyd
      181 qvlhedettn rmqeslklfd sicnnkwftd tsiilflnkk dlfeekirks plticfpeya
      241 gaqeygeaaa yiqaqfeakn ksttkeiych mtcatdtnni qfvfdavtdv iiannlrgcg
      301 ly
//
LOCUS       XP_076545504             583 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305491 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545504
VERSION     XP_076545504.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689389.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..583
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305491"
                     /calculated_mol_wt=65495
     CDS             1..583
                     /gene="LOC143305491"
                     /coded_by="XM_076689389.1:1..1752"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:143305491"
ORIGIN      
        1 mdkkskrgsn frpdrqrkrk aprnrysmeq dtsstsasaa klksskddei iiennygyci
       61 leffsvftal saliicaeck knikffrtap rglgfkiavq cscdgvqyin sspfinkafe
      121 inrrivvvmr llgvgregin ifcsmmdicq glaintyysc lenlqiasra hnvyehiisk
      181 avaeekelte asepntdpth ltvsgdgtwk krgfsslfgv ttlvgkyskk vidtvlkssf
      241 cqgcnlwrhk knedvaaynd wyeehedsct inhrgspgkm evdaviemfe rsvekhhaky
      301 akyigdgdak tfkgiididp yhgdpvvikk ecvghvqkrm garlrkikkd nkgiggkgpg
      361 kltdklidel slyyglairr npesvddmrn evwatyyhks stdenpqhky cppgesswck
      421 wqkavsegil qdfvhenppl sekvlelikp iyenlssenl ltrclgsetq nnnesfnsli
      481 wtfapkhiha gsdtieianh iavcifnegf lpvlkildtm sikigpesha faskxdqrri
      541 nrseirasea skegrtarma ersaknvafe veegtlyeag mad
//
LOCUS       XP_034194572             202 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610851 [Osmia lignaria lignaria].
ACCESSION   XP_034194572
VERSION     XP_034194572.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338681.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194572.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="uncharacterized protein LOC117610851"
                     /calculated_mol_wt=23068
     Region          79..153
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..202
                     /gene="LOC117610851"
                     /coded_by="XM_034338681.2:385..993"
                     /db_xref="GeneID:117610851"
ORIGIN      
        1 meildgtivp hksiqdvytl vsgmsdkmed nfekiytait tislniqaid trlknmetqh
       61 nqhkskasav dtsniqksfp fknineviaf enilsentde ynkfmlyisr igggsakenl
      121 iriyrtlfsn evakvsswkg lrnhfkissl ksmlmgiqat tlaqyqftnk efeditkewf
      181 rqggqrlsrq qkdseevnpq ai
//
LOCUS       XP_034184664             362 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184664
VERSION     XP_034184664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..362
                     /product="RNA-binding protein squid isoform X1"
                     /calculated_mol_wt=38823
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..362
                     /gene="sqd"
                     /coded_by="XM_034328773.2:148..1236"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyyggg yggyggydys gydgygyggg
      301 sydggysggr ggargkgfls ynkshnslnh hsikqrhlqq dfqqrrqlpq nkmnhqesrq
      361 ks
//
LOCUS       XP_034184665             348 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184665
VERSION     XP_034184665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328774.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..348
                     /product="RNA-binding protein squid isoform X2"
                     /calculated_mol_wt=37406
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..348
                     /gene="sqd"
                     /coded_by="XM_034328774.2:147..1193"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyygdg ygygggsydg gysggrggar
      301 gkgflsynks hnslnhhsik qrhlqqdfqq rrqlpqnkmn hqesrqks
//
LOCUS       XP_034184666             338 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034184666
VERSION     XP_034184666.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328775.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="RNA-binding protein squid isoform X3"
                     /calculated_mol_wt=35528
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..338
                     /gene="sqd"
                     /coded_by="XM_034328775.2:147..1163"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyyggg yggyggydys gydgygyggg
      301 sydggysggr ggargkggsg yggkqrgggr qnqrhqpy
//
LOCUS       XP_034184667             338 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034184667
VERSION     XP_034184667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="RNA-binding protein squid isoform X4"
                     /calculated_mol_wt=36570
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..338
                     /gene="sqd"
                     /coded_by="XM_034328776.2:147..1163"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyyggg yggyggydys gygflsynks
      301 hnslnhhsik qrhlqqdfqq rrqlpqnkmn hqesrqks
//
LOCUS       XP_034184668             324 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034184668
VERSION     XP_034184668.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328777.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..324
                     /product="RNA-binding protein squid isoform X5"
                     /calculated_mol_wt=34111
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..324
                     /gene="sqd"
                     /coded_by="XM_034328777.2:147..1121"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyygdg ygygggsydg gysggrggar
      301 gkggsgyggk qrgggrqnqr hqpy
//
LOCUS       XP_034184669             324 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034184669
VERSION     XP_034184669.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328778.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..324
                     /product="RNA-binding protein squid isoform X6"
                     /calculated_mol_wt=35152
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..324
                     /gene="sqd"
                     /coded_by="XM_034328778.2:147..1121"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyyggf lsynkshnsl nhhsikqrhl
      301 qqdfqqrrql pqnkmnhqes rqks
//
LOCUS       XP_034184672             314 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034184672
VERSION     XP_034184672.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328781.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..314
                     /product="RNA-binding protein squid isoform X7"
                     /calculated_mol_wt=33275
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..314
                     /gene="sqd"
                     /coded_by="XM_034328781.2:147..1091"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyyggg yggyggydys gyggsgyggk
      301 qrgggrqnqr hqpy
//
LOCUS       XP_034184673             300 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein squid isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034184673
VERSION     XP_034184673.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328782.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="RNA-binding protein squid isoform X8"
                     /calculated_mol_wt=31858
     Region          56..127
                     /region_name="RRM1_hnRNPA_hnRNPD_like"
                     /note="RNA recognition motif 1 (RRM1) found in
                     heterogeneous nuclear ribonucleoprotein hnRNP A and hnRNP
                     D subfamilies and similar proteins; cd12325"
                     /db_xref="CDD:409763"
     Site            order(57,59..62,84,86,95..97,99,122,125,127)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409763"
     Region          135..209
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..300
                     /gene="sqd"
                     /coded_by="XM_034328782.2:147..1049"
                     /db_xref="GeneID:117606402"
ORIGIN      
        1 madqenkdfs ediadqnfeq ngeaengggd aaengqesqe drstggnqds lndrklfvgg
       61 lswettdkel rdhfgtygdi esinvktdpn tgrsrgfafi vfakaesldk imsagdhiin
      121 nkkvdpkkak arhgkifvgg lstelsdedi knffsqfgti vdvempfdkt knqrkgfcfi
      181 tfeseqvvne llktpkqtin gkevdvkkat pkpdgmggmr ggaggrggrg grggrgrgfg
      241 gqggwgqggy gggygggygq ggygggydgy gggydyyggg sgyggkqrgg grqnqrhqpy
//
LOCUS       XP_076545505             501 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 6a2-like [Osmia lignaria lignaria].
ACCESSION   XP_076545505
VERSION     XP_076545505.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689390.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="cytochrome P450 6a2-like"
                     /calculated_mol_wt=57911
     Region          68..493
                     /region_name="CYP6-like"
                     /note="cytochrome P450 family 6 and similar cytochrome
                     P450s; cd11056"
                     /db_xref="CDD:410679"
     Site            order(128,132,139,186,300..301,304..305,308..309,312,364,
                     373,375,400,436..438,442..446,449..450)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410679"
     Site            order(212..213,300,303..304,308,371..374)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:410679"
     CDS             1..501
                     /gene="LOC117610862"
                     /coded_by="XM_076689390.1:298..1803"
                     /db_xref="GeneID:117610862"
ORIGIN      
        1 mlgyfeilcg iaallfafyy ystanfnywk drgvvgpkpl pffgntkdlm fatvsmpefs
       61 kdvylkykde rivglfmqrs pviflkdpel ikdvlikdfs kfsdrgfkvy ekteplsqhl
      121 fnletkrwrp mrsklspmft sgklrdmfgl ilecsehfkq yldkvvakge pidipdltgk
      181 fttdvigsca fgidtnslad eesefrkvgk qiftptlgnv lrletsiylp klyellgyiv
      241 pdkkfapfft kvvidtmmyr kehnihrpdf inmlmelkdh pqkiddiklt dyfitaqafv
      301 ffaagfetss ttmsnalyel aqnhdiqdrl rkeiekhfen sdgnfkyeqi kemeyldkvf
      361 ketlrkyppg allprkatsd ytfdgtkasi skgmsvwipi faihrdpeiy pnpevfdper
      421 fnedvvdarh pmsylpfgdg prncigarfa vyqtklglit ilrnykvdic ektvipyhft
      481 pgafiltpqe giylkitklt s
//
LOCUS       XP_076545506             501 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 6a2-like [Osmia lignaria lignaria].
ACCESSION   XP_076545506
VERSION     XP_076545506.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689391.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="cytochrome P450 6a2-like"
                     /calculated_mol_wt=57911
     Region          68..493
                     /region_name="CYP6-like"
                     /note="cytochrome P450 family 6 and similar cytochrome
                     P450s; cd11056"
                     /db_xref="CDD:410679"
     Site            order(128,132,139,186,300..301,304..305,308..309,312,364,
                     373,375,400,436..438,442..446,449..450)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410679"
     Site            order(212..213,300,303..304,308,371..374)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:410679"
     CDS             1..501
                     /gene="LOC117610862"
                     /coded_by="XM_076689391.1:137..1642"
                     /db_xref="GeneID:117610862"
ORIGIN      
        1 mlgyfeilcg iaallfafyy ystanfnywk drgvvgpkpl pffgntkdlm fatvsmpefs
       61 kdvylkykde rivglfmqrs pviflkdpel ikdvlikdfs kfsdrgfkvy ekteplsqhl
      121 fnletkrwrp mrsklspmft sgklrdmfgl ilecsehfkq yldkvvakge pidipdltgk
      181 fttdvigsca fgidtnslad eesefrkvgk qiftptlgnv lrletsiylp klyellgyiv
      241 pdkkfapfft kvvidtmmyr kehnihrpdf inmlmelkdh pqkiddiklt dyfitaqafv
      301 ffaagfetss ttmsnalyel aqnhdiqdrl rkeiekhfen sdgnfkyeqi kemeyldkvf
      361 ketlrkyppg allprkatsd ytfdgtkasi skgmsvwipi faihrdpeiy pnpevfdper
      421 fnedvvdarh pmsylpfgdg prncigarfa vyqtklglit ilrnykvdic ektvipyhft
      481 pgafiltpqe giylkitklt s
//
LOCUS       XP_076545507             501 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 6a2-like [Osmia lignaria lignaria].
ACCESSION   XP_076545507
VERSION     XP_076545507.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689392.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="cytochrome P450 6a2-like"
                     /calculated_mol_wt=57907
     Region          68..493
                     /region_name="CYP6-like"
                     /note="cytochrome P450 family 6 and similar cytochrome
                     P450s; cd11056"
                     /db_xref="CDD:410679"
     Site            order(128,132,139,186,300..301,304..305,308..309,312,364,
                     373,375,400,436..438,442..446,449..450)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410679"
     Site            order(212..213,300,303..304,308,371..374)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:410679"
     CDS             1..501
                     /gene="LOC117610864"
                     /coded_by="XM_076689392.1:419..1924"
                     /db_xref="GeneID:117610864"
ORIGIN      
        1 mlgyfeilcg iaallyalyy ystvnfnywk drgvvgpkpl pffgntkdlm fttvsmpefs
       61 kdiylkykde rmvglfvqrs pviflkdpel ikdvlikdfs kfsdrgfkvy ekteplsqhl
      121 fnletkrwrp mrsklspmft sgklrdmfgl ilecsehfkq yldkvvakge pidipdltgk
      181 fttdvigsca fgidtnslad eesefrkvgk qiftptlgnv ltlktsiylp klyellgyiv
      241 pdkkfapfft kvvidtmmyr kehnihrpdf inmlmelkdh pqkiddiklt dyfitaqafv
      301 ffaagfetss ttmsnalyel aqnhdiqdrl rkeiekhfek sdgnfkyeqi kemeyldkvf
      361 ketlrkyppg allprkatsd ytfdgtkasi skgmlvwipi faihrdpeiy pnpevfdper
      421 fnedavdarh pmsylpfgdg prncigarfa vyqtklglit ilrnykvdic ektvipyhft
      481 pgafiltpqe giylkitklt s
//
LOCUS       XP_076545508             368 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305492 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545508
VERSION     XP_076545508.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689393.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..368
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305492"
                     /calculated_mol_wt=42247
     Region          <2..53
                     /region_name="HTH_Tnp_Tc5"
                     /note="Tc5 transposase DNA-binding domain; cl12115"
                     /db_xref="CDD:472278"
     Region          146..281
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     Region          302..363
                     /region_name="Transp_Tc5_C"
                     /note="Tc5 transposase C-terminal domain; pfam04236"
                     /db_xref="CDD:398081"
     CDS             1..368
                     /gene="LOC143305492"
                     /coded_by="XM_076689393.1:4577..5683"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:143305492"
ORIGIN      
        1 mfrdalergs ivhdidirkw aleakenidl ptfkagkwwi fklkklhgiv srkitkfvty
       61 rerensheid tvidnfiqvv kpninsysie nvfnsdesgf nlelhsgrtl aakgsktiea
      121 vvqsissxhs ytiqptvsan gkllsplyii lkessgqfgp rvkeslfnap nvhvsaaktg
      181 kltsehfktw fteifltnmg srnvllldsw sghcpnvlhd lvpegkevlf ltipkkatgr
      241 lxpldvlgfr iwknfiktfs dqvillnhdv ilhqrnniik lqslvhnqls sprfqnlfkh
      301 swyksgyiee rsaryenpvd ycftkqvdki hrcdiygela iircawckky lrakhffeef
      361 hycksyve
//
LOCUS       XP_076545509             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545509
VERSION     XP_076545509.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689394.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117611402 isoform X1"
                     /calculated_mol_wt=35363
     CDS             1..320
                     /gene="LOC117611402"
                     /coded_by="XM_076689394.1:3505..4467"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 mfeetteelf qieetdnted glniegttse neeliiektv tneleeqfcl psglsivdmq
       61 ymfaefqkig dhaknredcg irhlkitgik rsglrttlsv icdmckytge vhsepdesnk
      121 metnqcaiag tivnggsyaq meeflaamni psmskkefrk hndkivktli vaaaeemvaa
      181 aegekrlaie sgdivsgcgi phipvvadgs wikrsyrtgt ydsasgvgvi igyhtkkvlf
      241 igvrnkvcri crcaakkkke prkqicfknc rasqastame sdaivegfkt svekhrliys
      301 tliadgdssv ykkiidadpy
//
LOCUS       XP_076545510             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545510
VERSION     XP_076545510.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689395.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117611402 isoform X1"
                     /calculated_mol_wt=35363
     CDS             1..320
                     /gene="LOC117611402"
                     /coded_by="XM_076689395.1:3084..4046"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 mfeetteelf qieetdnted glniegttse neeliiektv tneleeqfcl psglsivdmq
       61 ymfaefqkig dhaknredcg irhlkitgik rsglrttlsv icdmckytge vhsepdesnk
      121 metnqcaiag tivnggsyaq meeflaamni psmskkefrk hndkivktli vaaaeemvaa
      181 aegekrlaie sgdivsgcgi phipvvadgs wikrsyrtgt ydsasgvgvi igyhtkkvlf
      241 igvrnkvcri crcaakkkke prkqicfknc rasqastame sdaivegfkt svekhrliys
      301 tliadgdssv ykkiidadpy
//
LOCUS       XP_076545511             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545511
VERSION     XP_076545511.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689396.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117611402 isoform X1"
                     /calculated_mol_wt=35363
     CDS             1..320
                     /gene="LOC117611402"
                     /coded_by="XM_076689396.1:3554..4516"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 mfeetteelf qieetdnted glniegttse neeliiektv tneleeqfcl psglsivdmq
       61 ymfaefqkig dhaknredcg irhlkitgik rsglrttlsv icdmckytge vhsepdesnk
      121 metnqcaiag tivnggsyaq meeflaamni psmskkefrk hndkivktli vaaaeemvaa
      181 aegekrlaie sgdivsgcgi phipvvadgs wikrsyrtgt ydsasgvgvi igyhtkkvlf
      241 igvrnkvcri crcaakkkke prkqicfknc rasqastame sdaivegfkt svekhrliys
      301 tliadgdssv ykkiidadpy
//
LOCUS       XP_076545512             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545512
VERSION     XP_076545512.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689397.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117611402 isoform X1"
                     /calculated_mol_wt=35363
     CDS             1..320
                     /gene="LOC117611402"
                     /coded_by="XM_076689397.1:3500..4462"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 mfeetteelf qieetdnted glniegttse neeliiektv tneleeqfcl psglsivdmq
       61 ymfaefqkig dhaknredcg irhlkitgik rsglrttlsv icdmckytge vhsepdesnk
      121 metnqcaiag tivnggsyaq meeflaamni psmskkefrk hndkivktli vaaaeemvaa
      181 aegekrlaie sgdivsgcgi phipvvadgs wikrsyrtgt ydsasgvgvi igyhtkkvlf
      241 igvrnkvcri crcaakkkke prkqicfknc rasqastame sdaivegfkt svekhrliys
      301 tliadgdssv ykkiidadpy
//
LOCUS       XP_076545513             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545513
VERSION     XP_076545513.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689398.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117611402 isoform X1"
                     /calculated_mol_wt=35363
     CDS             1..320
                     /gene="LOC117611402"
                     /coded_by="XM_076689398.1:3136..4098"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 mfeetteelf qieetdnted glniegttse neeliiektv tneleeqfcl psglsivdmq
       61 ymfaefqkig dhaknredcg irhlkitgik rsglrttlsv icdmckytge vhsepdesnk
      121 metnqcaiag tivnggsyaq meeflaamni psmskkefrk hndkivktli vaaaeemvaa
      181 aegekrlaie sgdivsgcgi phipvvadgs wikrsyrtgt ydsasgvgvi igyhtkkvlf
      241 igvrnkvcri crcaakkkke prkqicfknc rasqastame sdaivegfkt svekhrliys
      301 tliadgdssv ykkiidadpy
//
LOCUS       XP_076545514             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545514
VERSION     XP_076545514.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689399.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117611402 isoform X1"
                     /calculated_mol_wt=35363
     CDS             1..320
                     /gene="LOC117611402"
                     /coded_by="XM_076689399.1:3133..4095"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 mfeetteelf qieetdnted glniegttse neeliiektv tneleeqfcl psglsivdmq
       61 ymfaefqkig dhaknredcg irhlkitgik rsglrttlsv icdmckytge vhsepdesnk
      121 metnqcaiag tivnggsyaq meeflaamni psmskkefrk hndkivktli vaaaeemvaa
      181 aegekrlaie sgdivsgcgi phipvvadgs wikrsyrtgt ydsasgvgvi igyhtkkvlf
      241 igvrnkvcri crcaakkkke prkqicfknc rasqastame sdaivegfkt svekhrliys
      301 tliadgdssv ykkiidadpy
//
LOCUS       XP_076545515             169 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545515
VERSION     XP_076545515.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689400.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..169
                     /product="uncharacterized protein LOC117611402 isoform X2"
                     /calculated_mol_wt=19314
     CDS             1..169
                     /gene="LOC117611402"
                     /coded_by="XM_076689400.1:797..1306"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 maeslhqfrw trkkrkkrkr ieetgerglt trkekeefea vdvatvcsgi etssevansv
       61 pvfreegfie essflvdvsl cgnenckqie lgkryypqpa dcghlrildr fcvqsgevrn
      121 arqaidvhgf lshlqprsrv rrikmlgagp skrgqqggrq hgfrldnrw
//
LOCUS       XP_076545516             136 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611402 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545516
VERSION     XP_076545516.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689401.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="uncharacterized protein LOC117611402 isoform X3"
                     /calculated_mol_wt=15329
     CDS             1..136
                     /gene="LOC117611402"
                     /coded_by="XM_076689401.1:790..1200"
                     /db_xref="GeneID:117611402"
ORIGIN      
        1 mqkwrnrcin savcsgiets sevansvpvf reegfieess flvdvslcgn enckqielgk
       61 ryypqpadcg hlrildrfcv qsgevrnarq aidvhgflsh lqprsrvrri kmlgagpskr
      121 gqqggrqhgf rldnrw
//
LOCUS       XP_034194585             110 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610866 [Osmia lignaria lignaria].
ACCESSION   XP_034194585
VERSION     XP_034194585.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338694.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194585.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..110
                     /product="uncharacterized protein LOC117610866"
                     /calculated_mol_wt=12053
     CDS             1..110
                     /gene="LOC117610866"
                     /coded_by="XM_034338694.2:1876..2208"
                     /db_xref="GeneID:117610866"
ORIGIN      
        1 mdctmaesqg nselsenpep nheqinqgrk esnekpneei nkqliqllat ykkqnaelkk
       61 evkrlvkysr gargegrgrg ggegrgrggr grgggkwggy vknnptiifk
//
LOCUS       XP_034194587             110 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610866 [Osmia lignaria lignaria].
ACCESSION   XP_034194587
VERSION     XP_034194587.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338696.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194587.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..110
                     /product="uncharacterized protein LOC117610866"
                     /calculated_mol_wt=12053
     CDS             1..110
                     /gene="LOC117610866"
                     /coded_by="XM_034338696.2:1893..2225"
                     /db_xref="GeneID:117610866"
ORIGIN      
        1 mdctmaesqg nselsenpep nheqinqgrk esnekpneei nkqliqllat ykkqnaelkk
       61 evkrlvkysr gargegrgrg ggegrgrggr grgggkwggy vknnptiifk
//
LOCUS       XP_034194588             110 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610866 [Osmia lignaria lignaria].
ACCESSION   XP_034194588
VERSION     XP_034194588.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338697.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194588.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..110
                     /product="uncharacterized protein LOC117610866"
                     /calculated_mol_wt=12053
     CDS             1..110
                     /gene="LOC117610866"
                     /coded_by="XM_034338697.2:3156..3488"
                     /db_xref="GeneID:117610866"
ORIGIN      
        1 mdctmaesqg nselsenpep nheqinqgrk esnekpneei nkqliqllat ykkqnaelkk
       61 evkrlvkysr gargegrgrg ggegrgrggr grgggkwggy vknnptiifk
//
LOCUS       XP_076545517             438 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305494 [Osmia lignaria lignaria].
ACCESSION   XP_076545517
VERSION     XP_076545517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689402.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..438
                     /product="uncharacterized protein LOC143305494"
                     /calculated_mol_wt=50460
     CDS             1..438
                     /gene="LOC143305494"
                     /coded_by="XM_076689402.1:11..1327"
                     /db_xref="GeneID:143305494"
ORIGIN      
        1 mnthkctenn hdlisehsei sidknspqss issdkfskte ccktelindi rlwaienrvs
       61 hsaissllki lvkyghnlpi dartlfqipk qvaiiemlpg kyyhaglgds lkksirkhyc
      121 rsnlpsqini nvnidglplt kpsgsqfwpi lgsivadfyt epfiigvyhg fekskcsntf
      181 lkyfkddcfk ifrtgiieyg kkitvklnvf icdapaksfi kgikghnayf gcgnyiqegd
      241 yienrvtype inamlrsdes fklrkqeehh kitsnledln igmvtqfpld ymhlvllgvm
      301 kkllqfwikg khnvrmvatq itlastslls mssflpkefa rkprkldevd rfkateceea
      361 qesrnkdikk frehhsrkfs riktiediyh nllvssdpyi sstrkvwpkk gktyppdviq
      421 ffdipnilef eeeeeeie
//
LOCUS       XP_034190390            1085 aa            linear   INV 03-SEP-2025
DEFINITION  strumpellin and WASH-interacting protein [Osmia lignaria lignaria].
ACCESSION   XP_034190390
VERSION     XP_034190390.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334499.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1085
                     /product="strumpellin and WASH-interacting protein"
                     /calculated_mol_wt=124417
     Region          28..556
                     /region_name="WASH-4_N"
                     /note="WASH complex subunit 4, N-terminal; pfam14745"
                     /db_xref="CDD:434178"
     Region          563..899
                     /region_name="WASH-7_mid"
                     /note="WASH complex subunit 7; pfam14744"
                     /db_xref="CDD:434177"
     Region          916..1081
                     /region_name="WASH-7_C"
                     /note="WASH complex subunit 7, C-terminal; pfam14746"
                     /db_xref="CDD:464293"
     CDS             1..1085
                     /gene="SWIP"
                     /coded_by="XM_034334499.2:362..3619"
                     /db_xref="GeneID:117608844"
ORIGIN      
        1 miessawnsr kdetiykaag aihlrkygef fenlaektwk kdstleyfie gplrlvykpv
       61 edislislie menkflskll aavagtcrei rlleeeakvf ykklfthger gtednhynit
      121 sllsdlqdlf vfvnristvv rltteqlssl sgdsnevylp vliehfislf tivltldeiv
      181 esqpslleqw kkyrmhvrsv thnptqfgis esklhmfdkl lkdlqehllk kvlfsktieh
      241 vmdvskgpvm seqitsylkn lmsdiesktn dnmavnknwv rvnigivvvt klfgtcdkrl
      301 ikrvleinkk cyavtlvgnv mwipskflsd flpkefgnaa ssqvgekvlg srtqklsqtv
      361 nhlihravtw cmemqsilvk tglqvsdigq kqsllvdlvn llsqikeiis fimnmhatli
      421 kpmnrntvql icrlievqks lqtmfyllgp iivqsqsqvl qylsyyilit letarksliq
      481 kdkgyskekl dtlsliglsm rllngpasad rrliirctla cagqltdtfr eedtiklrfl
      541 ldnydiiadl hniisefcdy silmhhqnii payfsfvtdr nvsvnhivhl fnafnstise
      601 qegsdlknkr itqlremltk nilepvchei etnlrlhvha hlkldstnpf nigakdggri
      661 vralplplan smiyakrfve hylddmfynl ttvalhdwkt yrmmhalayy klnldtvqnh
      721 lptqtleqgl daleimrnih vfvskylynl ntqtfiehts nnkhlntigi rhiansirth
      781 gtgimsttvn fvyqflrikl htfsqflfde hiksrlmrdi rfiknqreng atpytyerae
      841 kfqkgirklg mspdglsyld qfrqlitqig nalgyvrlir sgglhassna isflpdinss
      901 vpfelmcknl nyntttqaaa kclendianl vrnftegiqy fkllvdvfas afrdteshhl
      961 qqfyaiippl tlsfvdnsis nkekmfkknq tgaaftddgf amgiayinal lnqsseldsl
     1021 hwfktveqhl naekqaaesk ndhgdeklqq traltlkria ertaefqlly yslsgarvff
     1081 kqsdi
//
LOCUS       XP_034190396             662 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190396
VERSION     XP_034190396.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190396.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..662
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X1"
                     /calculated_mol_wt=76707
     Region          61..>176
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(64,67..68,71..72,74,98,105..106,109..110,112..113,
                     139,142..143,146..147,150)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          64..91
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          96..130
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          138..166
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          221..520
                     /region_name="SET_SMYD4"
                     /note="SET domain (including iSET domain and post-SET
                     domain) found in SET and MYND domain-containing protein 4
                     (SMYD4) and similar proteins; cd10536"
                     /db_xref="CDD:380934"
     Site            order(236..238,255,417,423,425,427..431,454..458,466,478,
                     489..494,513..517)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380934"
     Site            order(236..238,427..430,454..458,491,513..517)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380934"
     Region          272..312
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     Site            order(435..436,444,454,490..491,493)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:380934"
     Site            order(460,515,517,520)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380934"
     CDS             1..662
                     /gene="LOC117608847"
                     /coded_by="XM_034334505.2:370..2358"
                     /db_xref="GeneID:117608847"
ORIGIN      
        1 mdlsralqlv isklkdtcis reklqredev iiqlrnnenl mkrihllleg yydkrdlksv
       61 dlgksyrkkg ndrfqkkhyt esiksytlca lysppssnel siayanrsac lfylnryndc
      121 indiklalnl nypinwcyki ylraaqcylk lgkrqlaeem lfkvqeminn pdyivaamkd
      181 hikkkiseit lsdssiepne qksnglskik sqikfeenen fsnasssidk kyneevgrhv
      241 vanrmikrge ilflekpvsf vsvnfdglsd ychhcncltr dvpvpckncl ntcycseecl
      301 seawslyhmw ecvasqmgll knigigrlal kvlltcstmt dtvrfnemhs llthfnkipv
      361 ddlidygvta lvltiylsky tdffklplee flmnkffddp lksvrddkql yvaslllkhi
      421 lqlvanahai sssntllshn tedivasgiy pstsmmnhsc npniitifmd qyiiikaatd
      481 iladeevfis ygphfrhmem ekrqeilksq fhftckckpc tslksckskd iervfylqnm
      541 dwqnkeplin imsaatkvhm esgrieegva cmdrtiaiak gryrffdtkl litlntfadl
      601 cleylqisnt nntykivqkh aeeylnemee mvnwnygsws kiyedvkikq ekivpvhkrf
      661 nm
//
LOCUS       XP_034190397             662 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190397
VERSION     XP_034190397.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334506.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190397.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..662
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X1"
                     /calculated_mol_wt=76707
     Region          61..>176
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(64,67..68,71..72,74,98,105..106,109..110,112..113,
                     139,142..143,146..147,150)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          64..91
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          96..130
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          138..166
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          221..520
                     /region_name="SET_SMYD4"
                     /note="SET domain (including iSET domain and post-SET
                     domain) found in SET and MYND domain-containing protein 4
                     (SMYD4) and similar proteins; cd10536"
                     /db_xref="CDD:380934"
     Site            order(236..238,255,417,423,425,427..431,454..458,466,478,
                     489..494,513..517)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380934"
     Site            order(236..238,427..430,454..458,491,513..517)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380934"
     Region          272..312
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     Site            order(435..436,444,454,490..491,493)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:380934"
     Site            order(460,515,517,520)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380934"
     CDS             1..662
                     /gene="LOC117608847"
                     /coded_by="XM_034334506.2:233..2221"
                     /db_xref="GeneID:117608847"
ORIGIN      
        1 mdlsralqlv isklkdtcis reklqredev iiqlrnnenl mkrihllleg yydkrdlksv
       61 dlgksyrkkg ndrfqkkhyt esiksytlca lysppssnel siayanrsac lfylnryndc
      121 indiklalnl nypinwcyki ylraaqcylk lgkrqlaeem lfkvqeminn pdyivaamkd
      181 hikkkiseit lsdssiepne qksnglskik sqikfeenen fsnasssidk kyneevgrhv
      241 vanrmikrge ilflekpvsf vsvnfdglsd ychhcncltr dvpvpckncl ntcycseecl
      301 seawslyhmw ecvasqmgll knigigrlal kvlltcstmt dtvrfnemhs llthfnkipv
      361 ddlidygvta lvltiylsky tdffklplee flmnkffddp lksvrddkql yvaslllkhi
      421 lqlvanahai sssntllshn tedivasgiy pstsmmnhsc npniitifmd qyiiikaatd
      481 iladeevfis ygphfrhmem ekrqeilksq fhftckckpc tslksckskd iervfylqnm
      541 dwqnkeplin imsaatkvhm esgrieegva cmdrtiaiak gryrffdtkl litlntfadl
      601 cleylqisnt nntykivqkh aeeylnemee mvnwnygsws kiyedvkikq ekivpvhkrf
      661 nm
//
LOCUS       XP_034190398             631 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190398
VERSION     XP_034190398.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334507.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190398.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X2"
                     /calculated_mol_wt=73067
     Region          61..>176
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(64,67..68,71..72,74,98,105..106,109..110,112..113,
                     139,142..143,146..147,150)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          64..91
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          96..130
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          138..166
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          221..520
                     /region_name="SET_SMYD4"
                     /note="SET domain (including iSET domain and post-SET
                     domain) found in SET and MYND domain-containing protein 4
                     (SMYD4) and similar proteins; cd10536"
                     /db_xref="CDD:380934"
     Site            order(236..238,255,417,423,425,427..431,454..458,466,478,
                     489..494,513..517)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380934"
     Site            order(236..238,427..430,454..458,491,513..517)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380934"
     Region          272..312
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     Site            order(435..436,444,454,490..491,493)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:380934"
     Site            order(460,515,517,520)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380934"
     CDS             1..631
                     /gene="LOC117608847"
                     /coded_by="XM_034334507.2:371..2266"
                     /db_xref="GeneID:117608847"
ORIGIN      
        1 mdlsralqlv isklkdtcis reklqredev iiqlrnnenl mkrihllleg yydkrdlksv
       61 dlgksyrkkg ndrfqkkhyt esiksytlca lysppssnel siayanrsac lfylnryndc
      121 indiklalnl nypinwcyki ylraaqcylk lgkrqlaeem lfkvqeminn pdyivaamkd
      181 hikkkiseit lsdssiepne qksnglskik sqikfeenen fsnasssidk kyneevgrhv
      241 vanrmikrge ilflekpvsf vsvnfdglsd ychhcncltr dvpvpckncl ntcycseecl
      301 seawslyhmw ecvasqmgll knigigrlal kvlltcstmt dtvrfnemhs llthfnkipv
      361 ddlidygvta lvltiylsky tdffklplee flmnkffddp lksvrddkql yvaslllkhi
      421 lqlvanahai sssntllshn tedivasgiy pstsmmnhsc npniitifmd qyiiikaatd
      481 iladeevfis ygphfrhmem ekrqeilksq fhftckckpc tslksckskd iervfylqnm
      541 dwqnkeplin imsaatkvhm esgrieegva cmdrtiaiak gryrivqkha eeylnemeem
      601 vnwnygswsk iyedvkikqe kivpvhkrfn m
//
LOCUS       XP_034190399             572 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190399
VERSION     XP_034190399.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334508.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190399.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X3"
                     /calculated_mol_wt=66072
     Region          61..>176
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(64,67..68,71..72,74,98,105..106,109..110,112..113,
                     139,142..143,146..147,150)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          64..91
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          96..130
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          138..166
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          221..520
                     /region_name="SET_SMYD4"
                     /note="SET domain (including iSET domain and post-SET
                     domain) found in SET and MYND domain-containing protein 4
                     (SMYD4) and similar proteins; cd10536"
                     /db_xref="CDD:380934"
     Site            order(236..238,255,417,423,425,427..431,454..458,466,478,
                     489..494,513..517)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380934"
     Site            order(236..238,427..430,454..458,491,513..517)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380934"
     Region          272..312
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     Site            order(435..436,444,454,490..491,493)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:380934"
     Site            order(460,515,517,520)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380934"
     CDS             1..572
                     /gene="LOC117608847"
                     /coded_by="XM_034334508.2:370..2088"
                     /db_xref="GeneID:117608847"
ORIGIN      
        1 mdlsralqlv isklkdtcis reklqredev iiqlrnnenl mkrihllleg yydkrdlksv
       61 dlgksyrkkg ndrfqkkhyt esiksytlca lysppssnel siayanrsac lfylnryndc
      121 indiklalnl nypinwcyki ylraaqcylk lgkrqlaeem lfkvqeminn pdyivaamkd
      181 hikkkiseit lsdssiepne qksnglskik sqikfeenen fsnasssidk kyneevgrhv
      241 vanrmikrge ilflekpvsf vsvnfdglsd ychhcncltr dvpvpckncl ntcycseecl
      301 seawslyhmw ecvasqmgll knigigrlal kvlltcstmt dtvrfnemhs llthfnkipv
      361 ddlidygvta lvltiylsky tdffklplee flmnkffddp lksvrddkql yvaslllkhi
      421 lqlvanahai sssntllshn tedivasgiy pstsmmnhsc npniitifmd qyiiikaatd
      481 iladeevfis ygphfrhmem ekrqeilksq fhftckckpc tslksckskd iervfylqnm
      541 dwqnkeplin imsaatkvhm eselyknmlk ni
//
LOCUS       XP_034190476             357 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608867 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190476
VERSION     XP_034190476.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334585.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190476.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..357
                     /product="uncharacterized protein LOC117608867 isoform X1"
                     /calculated_mol_wt=40013
     Region          239..307
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..357
                     /gene="LOC117608867"
                     /coded_by="XM_034334585.2:78..1151"
                     /db_xref="GeneID:117608867"
ORIGIN      
        1 mkrmlnvfis vngrqnmsan twtvvrfved gtveavpsaw mrgdlchwpp lhrnsltaai
       61 krsepvnlsw pthtiqtfkh gifdtyikar ekariaedis dlqsevetmr rrkrtrrfvs
      121 seseesesds ltlpspppmt egrkemttvg dsaagnaala saaaptvtat agpsssgcnd
      181 semckccrer nqyldtiikq nhlirgllmd ltadmkelkl ahkvgsvaeq pvslysryeg
      241 lkfpltreed llvletflak edhfkdaiqe lakiggdnny qfvkrvlssv idnnlalkys
      301 wlgrkgkkvf ctlhlarlvi saaetanlgd tkratevsiq nwlrrafdrn hitpkqf
//
LOCUS       XP_034190479             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608867 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190479
VERSION     XP_034190479.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334588.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190479.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117608867 isoform X2"
                     /calculated_mol_wt=38124
     Region          223..291
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..341
                     /gene="LOC117608867"
                     /coded_by="XM_034334588.2:243..1268"
                     /db_xref="GeneID:117608867"
ORIGIN      
        1 msantwtvvr fvedgtveav psawmrgdlc hwpplhrnsl taaikrsepv nlswpthtiq
       61 tfkhgifdty ikarekaria edisdlqsev etmrrrkrtr rfvssesees esdsltlpsp
      121 ppmtegrkem ttvgdsaagn aalasaaapt vtatagpsss gcndsemckc crernqyldt
      181 iikqnhlirg llmdltadmk elklahkvgs vaeqpvslys ryeglkfplt reedllvlet
      241 flakedhfkd aiqelakigg dnnyqfvkrv lssvidnnla lkyswlgrkg kkvfctlhla
      301 rlvisaaeta nlgdtkrate vsiqnwlrra fdrnhitpkq f
//
LOCUS       XP_034190478             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608867 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190478
VERSION     XP_034190478.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334587.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190478.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117608867 isoform X2"
                     /calculated_mol_wt=38124
     Region          223..291
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..341
                     /gene="LOC117608867"
                     /coded_by="XM_034334587.2:252..1277"
                     /db_xref="GeneID:117608867"
ORIGIN      
        1 msantwtvvr fvedgtveav psawmrgdlc hwpplhrnsl taaikrsepv nlswpthtiq
       61 tfkhgifdty ikarekaria edisdlqsev etmrrrkrtr rfvssesees esdsltlpsp
      121 ppmtegrkem ttvgdsaagn aalasaaapt vtatagpsss gcndsemckc crernqyldt
      181 iikqnhlirg llmdltadmk elklahkvgs vaeqpvslys ryeglkfplt reedllvlet
      241 flakedhfkd aiqelakigg dnnyqfvkrv lssvidnnla lkyswlgrkg kkvfctlhla
      301 rlvisaaeta nlgdtkrate vsiqnwlrra fdrnhitpkq f
//
LOCUS       XP_034190477             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608867 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190477
VERSION     XP_034190477.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334586.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190477.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117608867 isoform X2"
                     /calculated_mol_wt=38124
     Region          223..291
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..341
                     /gene="LOC117608867"
                     /coded_by="XM_034334586.2:463..1488"
                     /db_xref="GeneID:117608867"
ORIGIN      
        1 msantwtvvr fvedgtveav psawmrgdlc hwpplhrnsl taaikrsepv nlswpthtiq
       61 tfkhgifdty ikarekaria edisdlqsev etmrrrkrtr rfvssesees esdsltlpsp
      121 ppmtegrkem ttvgdsaagn aalasaaapt vtatagpsss gcndsemckc crernqyldt
      181 iikqnhlirg llmdltadmk elklahkvgs vaeqpvslys ryeglkfplt reedllvlet
      241 flakedhfkd aiqelakigg dnnyqfvkrv lssvidnnla lkyswlgrkg kkvfctlhla
      301 rlvisaaeta nlgdtkrate vsiqnwlrra fdrnhitpkq f
//
LOCUS       XP_076545518             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608867 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545518
VERSION     XP_076545518.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689403.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117608867 isoform X2"
                     /calculated_mol_wt=38124
     Region          223..291
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..341
                     /gene="LOC117608867"
                     /coded_by="XM_076689403.1:569..1594"
                     /db_xref="GeneID:117608867"
ORIGIN      
        1 msantwtvvr fvedgtveav psawmrgdlc hwpplhrnsl taaikrsepv nlswpthtiq
       61 tfkhgifdty ikarekaria edisdlqsev etmrrrkrtr rfvssesees esdsltlpsp
      121 ppmtegrkem ttvgdsaagn aalasaaapt vtatagpsss gcndsemckc crernqyldt
      181 iikqnhlirg llmdltadmk elklahkvgs vaeqpvslys ryeglkfplt reedllvlet
      241 flakedhfkd aiqelakigg dnnyqfvkrv lssvidnnla lkyswlgrkg kkvfctlhla
      301 rlvisaaeta nlgdtkrate vsiqnwlrra fdrnhitpkq f
//
LOCUS       XP_034190481             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608867 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190481
VERSION     XP_034190481.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334590.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190481.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117608867 isoform X2"
                     /calculated_mol_wt=38124
     Region          223..291
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..341
                     /gene="LOC117608867"
                     /coded_by="XM_034334590.2:404..1429"
                     /db_xref="GeneID:117608867"
ORIGIN      
        1 msantwtvvr fvedgtveav psawmrgdlc hwpplhrnsl taaikrsepv nlswpthtiq
       61 tfkhgifdty ikarekaria edisdlqsev etmrrrkrtr rfvssesees esdsltlpsp
      121 ppmtegrkem ttvgdsaagn aalasaaapt vtatagpsss gcndsemckc crernqyldt
      181 iikqnhlirg llmdltadmk elklahkvgs vaeqpvslys ryeglkfplt reedllvlet
      241 flakedhfkd aiqelakigg dnnyqfvkrv lssvidnnla lkyswlgrkg kkvfctlhla
      301 rlvisaaeta nlgdtkrate vsiqnwlrra fdrnhitpkq f
//
LOCUS       XP_076545519             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608867 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545519
VERSION     XP_076545519.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689404.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117608867 isoform X2"
                     /calculated_mol_wt=38124
     Region          223..291
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..341
                     /gene="LOC117608867"
                     /coded_by="XM_076689404.1:349..1374"
                     /db_xref="GeneID:117608867"
ORIGIN      
        1 msantwtvvr fvedgtveav psawmrgdlc hwpplhrnsl taaikrsepv nlswpthtiq
       61 tfkhgifdty ikarekaria edisdlqsev etmrrrkrtr rfvssesees esdsltlpsp
      121 ppmtegrkem ttvgdsaagn aalasaaapt vtatagpsss gcndsemckc crernqyldt
      181 iikqnhlirg llmdltadmk elklahkvgs vaeqpvslys ryeglkfplt reedllvlet
      241 flakedhfkd aiqelakigg dnnyqfvkrv lssvidnnla lkyswlgrkg kkvfctlhla
      301 rlvisaaeta nlgdtkrate vsiqnwlrra fdrnhitpkq f
//
LOCUS       XP_034190474             256 aa            linear   INV 03-SEP-2025
DEFINITION  CUE domain-containing protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190474
VERSION     XP_034190474.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334583.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="CUE domain-containing protein 1"
                     /calculated_mol_wt=29404
     Region          32..73
                     /region_name="CUE_CUED1"
                     /note="CUE domain found in CUE domain-containing protein 1
                     (CUED1) and similar proteins; cd14366"
                     /db_xref="CDD:270549"
     CDS             1..256
                     /gene="LOC117608866"
                     /coded_by="XM_034334583.2:594..1364"
                     /db_xref="GeneID:117608866"
ORIGIN      
        1 masameqqqq qqqqqqqqqq qqqqqqqqtt lefyqamadf knmfpqmddd vieavlrsnq
       61 gavdttidql ltmstdnene kirseleqse kspgtsklst idtnvslksi rkwqpamlgp
      121 lpdtflrvsq qvpedsldts ylqensmled eriamflqne efmaelrwne dflstlendt
      181 kiqgtnlekc tghinhdded lfkerlknmg kmsrrkfaql tkvftrskkr ggrqllppta
      241 scddlldpeg ssrsqp
//
LOCUS       XP_034190475             256 aa            linear   INV 03-SEP-2025
DEFINITION  CUE domain-containing protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190475
VERSION     XP_034190475.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334584.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="CUE domain-containing protein 1"
                     /calculated_mol_wt=29404
     Region          32..73
                     /region_name="CUE_CUED1"
                     /note="CUE domain found in CUE domain-containing protein 1
                     (CUED1) and similar proteins; cd14366"
                     /db_xref="CDD:270549"
     CDS             1..256
                     /gene="LOC117608866"
                     /coded_by="XM_034334584.2:323..1093"
                     /db_xref="GeneID:117608866"
ORIGIN      
        1 masameqqqq qqqqqqqqqq qqqqqqqqtt lefyqamadf knmfpqmddd vieavlrsnq
       61 gavdttidql ltmstdnene kirseleqse kspgtsklst idtnvslksi rkwqpamlgp
      121 lpdtflrvsq qvpedsldts ylqensmled eriamflqne efmaelrwne dflstlendt
      181 kiqgtnlekc tghinhdded lfkerlknmg kmsrrkfaql tkvftrskkr ggrqllppta
      241 scddlldpeg ssrsqp
//
LOCUS       XP_034184904            1258 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase [Osmia lignaria lignaria].
ACCESSION   XP_034184904
VERSION     XP_034184904.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329013.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184904.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1258
                     /product="activated Cdc42 kinase"
                     /calculated_mol_wt=139983
     Region          6..67
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          115..370
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(116..118,124,140,142,189..192,196,236,240..241,243,
                     254,273..277,286,320)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(116..118,124,140,142,189..192,196,236,240..241,243,
                     254)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(236,240,273..277,286,320)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(253..263,265..279)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          377..427
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(381,383,388,406,408,423,425..426)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212896"
     Region          433..>482
                     /region_name="GTPase_binding"
                     /note="GTPase binding; pfam09027"
                     /db_xref="CDD:430374"
     Region          1216..1254
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     CDS             1..1258
                     /gene="Ack"
                     /coded_by="XM_034329013.2:647..4423"
                     /db_xref="GeneID:117606507"
ORIGIN      
        1 maddegtewl qellhdvqls qfftrirddl qvtrlhhfdy vqsedlekig lgkpgirrll
       61 davkkkrttq wkkslmtkir pgsstksnkr ssqpvegssv ltcliqdkdv mlsiklgdgs
      121 fgvvrkgewt spsgrvlpva vkvlkadalt qpsviedfvs evqamhtldh hnlirlygvv
      181 lsqpmmmvte laplgalldy lrkqggrisv lticnyalqv atgmayleak rflhrdlacr
      241 nvllstvdkv kigdfglmra lpqqedcyvm tehkkvpfpw capeslkarq fshasdvwmf
      301 gvtlwemltf geepwvglng seilrkidre gerlhepeat ppvmyqlmlr cwarepserp
      361 tfaslrtslt gmvpavmkav nhfeetdkmt ieqgdqivil dgrpenywwk gqnqrtfqig
      421 pfprclvdpm rrkqpedisk plensfihtg hgapfgkswg spiyiddvyl rnpmeppdiv
      481 gvatapdshl kkkfscgtpr srkqfnytkl qndiraspvk stntsapsss qegslidlsp
      541 eeivntnvaq sdtacrrvvn ildepiddme rqqtnwqedd prtyanfpgn vdpaysdpfd
      601 tssvfmklph sryyshvpsd vssryfktqt ygsvqnqdas gsqssanyfa pesgsdvnhy
      661 sinlskenrn dsinlnvnvd seennaysqn hyteidkpsp pmanwsnwpe dmqtesqtyv
      721 nvtsrnvpnt evpppppvap nespvkpksn hdlaqtlsel tinacnvspk kldpaflael
      781 ekhlgekeas knmnasqqnp essdsyssvn klqentipal rpppqsakpk spqidyrsnn
      841 lpskvqnswq sktsniqqpr iqpadqqrvl etsteqmvgq iwqqaqtsqh stygntqanl
      901 nllqiapsnl snqcnvsktn fnhgqnslpt asqavhiqnl plsstthgit qiqndlqqah
      961 ssnvvpkpat mvfseqvyae lkqtvpnleq lsqnefntly nktvqqnilr nhyaasgssa
     1021 snstsnlshn hhpegtagss qvrsgparnl svqshlcdfs phlkqppvyn ppppawspmk
     1081 svqnglvqih svaaksnlas nlatnsnllq lthtnvpqeg avtpqtsvtr vlppqmnetv
     1141 vntqlapsas aypsgvsppl taasqqlvms lndefrankv mkvqreatda sqqeiltalq
     1201 atgwdtnqaa kqilkdrqak veslfrlgla drqqcegalk qteydvdlaa svlldqvr
//
LOCUS       XP_034184936             231 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606513 [Osmia lignaria lignaria].
ACCESSION   XP_034184936
VERSION     XP_034184936.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329045.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..231
                     /product="uncharacterized protein LOC117606513"
                     /calculated_mol_wt=26952
     CDS             1..231
                     /gene="LOC117606513"
                     /coded_by="XM_034329045.2:1230..1925"
                     /db_xref="GeneID:117606513"
ORIGIN      
        1 mkmpaessiy wdsvgsqrst sggstgsasr hyvdpwdlen yaylrrhsva gltqqasrhh
       61 rhqahrmsqe srahseywys sstrepgyva pafveelycg pvrptnnscy yhqpiyedev
      121 pdyvapfpvy aplsevrhgk yvedhrrhrh rckstpikdt hrsmyvnhmp pmdtveldiq
      181 ehhprteyrs leqeecmmhi vppphldlnt yghlkidytn swnslnrkis k
//
LOCUS       XP_034184942             221 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606516 [Osmia lignaria lignaria].
ACCESSION   XP_034184942
VERSION     XP_034184942.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329051.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184942.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="uncharacterized protein LOC117606516"
                     /calculated_mol_wt=26144
     CDS             1..221
                     /gene="LOC117606516"
                     /coded_by="XM_034329051.2:33..698"
                     /db_xref="GeneID:117606516"
ORIGIN      
        1 meveeyvsye fqgileefgr irdayvalka yckiqdetmt mekersermr enleklsrty
       61 lllerrckst ivalqtekeg llktiedlke qcdhlrlins ernanderty qlqdeieilk
      121 aqlsmqeekh nediallkqk hsdeiqrykm llqntkkntt kpknlvkskn ntscfrwpel
      181 nierhksnlm diknestgsk sqgnkkrklf sedrtatfdi v
//
LOCUS       XP_076545521            1139 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate cyclase type 6 [Osmia lignaria lignaria].
ACCESSION   XP_076545521
VERSION     XP_076545521.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689406.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1139
                     /product="adenylate cyclase type 6"
                     /calculated_mol_wt=127276
     Region          24..423
                     /region_name="AC_N"
                     /note="Adenylyl cyclase N-terminal extracellular and
                     transmembrane region; pfam16214"
                     /db_xref="CDD:318454"
     Region          425..609
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(437,439..444,479,481..483,486,548..550,554..555,
                     558..559,596)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(439,483)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(444,456,459..460,463,467,479..480,536,539,549..552,
                     555,596)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          <656..711
                     /region_name="Adcy_cons_dom"
                     /note="Adenylate cyclase, conserved domain; pfam06327"
                     /db_xref="CDD:461877"
     Region          <850..1102
                     /region_name="AcyC"
                     /note="Adenylate cyclase, class 3 [Signal transduction
                     mechanisms]; COG2114"
                     /db_xref="CDD:441717"
     Region          975..>1097
                     /region_name="Nucleotidyl_cyc_III"
                     /note="Class III nucleotidyl cyclases; cl11967"
                     /db_xref="CDD:448371"
     Site            order(989,1040)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     CDS             1..1139
                     /gene="LOC117608931"
                     /coded_by="XM_076689406.1:540..3959"
                     /db_xref="GeneID:117608931"
ORIGIN      
        1 mekygvelpk hewshrlslr asrrshqgev aarsvggpar strrwaslrq hvssndggkp
       61 kvellldppg sksstpssqp htpntpktph tplrksnwev iehftgaprp siitmnrgse
      121 igvgltgnsv eatqevnvnt pitqparkca lcrflkslca shrfknlqve mlyqryflrm
      181 nqsntthvlg llaglalalg lllilrille dnqqffvtle rpenlslavt llicimvyaa
      241 lvaaisrpgm neiwlagvsg ivlvtllalq lalnihlalg skehrsggpl aaawavcffi
      301 ymayallpir lrhaciagfi fslahligaf alypshypam mehllssivv vggtnmagvl
      361 thhprelaqr qafletrqcv earlttqren qqqerlllsv lprhvamemk adiagkpkdt
      421 mfhkiyiqrh envsilfadi cgftslsdqc taeevvrlln klfacfdtla aehhclrikl
      481 lgdcyycvsg lpeprpdhar ccvemgldmi saitlyrevg avnvnmrvgi htgrvhcgvl
      541 glrkwqfdvw sndvtlanym esggipgrvh itketldclg gyyeveegqg gernaylkeh
      601 niktyliipg dtykgnipss glrkglpang nvskemrvmg hgsqrgkqak lgfgetaesk
      661 kdpedevney lmraidarsi drlraeyctp filtfrrpdv eekysrerdr mlsayfvcsg
      721 fvytvvviai aitlygsvyf ytcvgvsill iasingilla eknervprcl rnfsvhvfen
      781 rgvaqalatc vvfltfvtal splltleggr vcsngtatfl ewpdnssvtf qgidapvdvc
      841 yytvfadlfp vlvmlvmitc avyqiltsvf kvailslvva tyfaisihea lnekdveltg
      901 rwlagvlvvf fmaclvahsq hteatyrldf lwklqateek eemehlkayn qkllanilpe
      961 hvaahflstv nsdelyheqc dfvcimfasi pnfsefyvel eannegvecl rllneiiadf
     1021 dellaeerfk yiekikstga tymaasgltr sacdmkdykh vtamaeyair ireqlasvne
     1081 hsfnnfrlrv ginigpvtsi nfpelsrchr etklarifhq tfgrcrgdrr pkaavrylg
//
LOCUS       XP_034190592             522 aa            linear   INV 03-SEP-2025
DEFINITION  putative peptidoglycan muropeptide transporter SLC46 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190592
VERSION     XP_034190592.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334701.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="putative peptidoglycan muropeptide transporter
                     SLC46 isoform X1"
                     /calculated_mol_wt=59220
     Region          49..471
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(58..59,62..63,66,125,181..182,184..186,189,211,
                     214..215,218,312,315..316,319..321,324,350,354,405..406,
                     410,414,430,433..434,437..438,441)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340944"
     CDS             1..522
                     /gene="LOC117608933"
                     /coded_by="XM_034334701.2:353..1921"
                     /db_xref="GeneID:117608933"
ORIGIN      
        1 mkqqvngrts seskynfhmr dsnkeclkng dsemthrkwr kyfewvdkvs veptmwlymm
       61 afmitsvveq affvykscrv dhgyseevca rlndnetika kvqvtvsefh qwnniaghvv
      121 piilalffgn wsdrrgrklp milgllgkfv ysfmvvinsm vdswnlntvv ytaslpmgml
      181 ggdvaifgsc fayisdissv qqrtlritil divylstmpt gvalgsylyt kvanssysim
      241 ftinasllal aiiysiirlk wqtlpqqqpl sgtnlltdff dkkhvvatmk tmtrrrpnhg
      301 klrlwlllti mmlytfqrde kpmsflytql kfkwdvakfs dfktfqsttf vlamligvpv
      361 msklmgikdt iivaigaiah aagrvlfvla nvpelfyvga avaalgpvva pvlrsmtskl
      421 vpveergkvf amlsvcdnav plfsgilysq lynatintap ssiywltfft qilvlllili
      481 ihffmrndlq leeqeftnte tdcrslsstv etdksnsnee ky
//
LOCUS       XP_034190593             522 aa            linear   INV 03-SEP-2025
DEFINITION  putative peptidoglycan muropeptide transporter SLC46 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190593
VERSION     XP_034190593.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334702.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="putative peptidoglycan muropeptide transporter
                     SLC46 isoform X1"
                     /calculated_mol_wt=59220
     Region          49..471
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(58..59,62..63,66,125,181..182,184..186,189,211,
                     214..215,218,312,315..316,319..321,324,350,354,405..406,
                     410,414,430,433..434,437..438,441)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340944"
     CDS             1..522
                     /gene="LOC117608933"
                     /coded_by="XM_034334702.2:582..2150"
                     /db_xref="GeneID:117608933"
ORIGIN      
        1 mkqqvngrts seskynfhmr dsnkeclkng dsemthrkwr kyfewvdkvs veptmwlymm
       61 afmitsvveq affvykscrv dhgyseevca rlndnetika kvqvtvsefh qwnniaghvv
      121 piilalffgn wsdrrgrklp milgllgkfv ysfmvvinsm vdswnlntvv ytaslpmgml
      181 ggdvaifgsc fayisdissv qqrtlritil divylstmpt gvalgsylyt kvanssysim
      241 ftinasllal aiiysiirlk wqtlpqqqpl sgtnlltdff dkkhvvatmk tmtrrrpnhg
      301 klrlwlllti mmlytfqrde kpmsflytql kfkwdvakfs dfktfqsttf vlamligvpv
      361 msklmgikdt iivaigaiah aagrvlfvla nvpelfyvga avaalgpvva pvlrsmtskl
      421 vpveergkvf amlsvcdnav plfsgilysq lynatintap ssiywltfft qilvlllili
      481 ihffmrndlq leeqeftnte tdcrslsstv etdksnsnee ky
//
LOCUS       XP_034190594             497 aa            linear   INV 03-SEP-2025
DEFINITION  putative peptidoglycan muropeptide transporter SLC46 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190594
VERSION     XP_034190594.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334703.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="putative peptidoglycan muropeptide transporter
                     SLC46 isoform X2"
                     /calculated_mol_wt=56292
     Region          24..446
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(33..34,37..38,41,100,156..157,159..161,164,186,
                     189..190,193,287,290..291,294..296,299,325,329,380..381,
                     385,389,405,408..409,412..413,416)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340944"
     CDS             1..497
                     /gene="LOC117608933"
                     /coded_by="XM_034334703.2:353..1846"
                     /db_xref="GeneID:117608933"
ORIGIN      
        1 mkqqgdsemt hrkwrkyfew vdkvsveptm wlymmafmit svveqaffvy kscrvdhgys
       61 eevcarlndn etikakvqvt vsefhqwnni aghvvpiila lffgnwsdrr grklpmilgl
      121 lgkfvysfmv vinsmvdswn lntvvytasl pmgmlggdva ifgscfayis dissvqqrtl
      181 ritildivyl stmptgvalg sylytkvans sysimftina sllalaiiys iirlkwqtlp
      241 qqqplsgtnl ltdffdkkhv vatmktmtrr rpnhgklrlw llltimmlyt fqrdekpmsf
      301 lytqlkfkwd vakfsdfktf qsttfvlaml igvpvmsklm gikdtiivai gaiahaagrv
      361 lfvlanvpel fyvgaavaal gpvvapvlrs mtsklvpvee rgkvfamlsv cdnavplfsg
      421 ilysqlynat intapssiyw ltfftqilvl lliliihffm rndlqleeqe ftntetdcrs
      481 lsstvetdks nsneeky
//
LOCUS       XP_034190300            3997 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 13D isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190300
VERSION     XP_034190300.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334409.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190300.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3997
                     /product="vacuolar protein sorting 13D isoform X1"
                     /calculated_mol_wt=447780
     Region          1..>935
                     /region_name="MRS6"
                     /note="Vacuolar protein sorting-associated protein
                     [Intracellular trafficking and secretion]; COG5043"
                     /db_xref="CDD:227376"
     Region          <1611..>2748
                     /region_name="MRS6"
                     /note="Vacuolar protein sorting-associated protein
                     [Intracellular trafficking and secretion]; COG5043"
                     /db_xref="CDD:227376"
     Region          2874..3152
                     /region_name="SHR-BD"
                     /note="SHR-binding domain of vacuolar-sorting associated
                     protein 13; pfam06650"
                     /db_xref="CDD:461974"
     Region          3212..3370
                     /region_name="beta-trefoil_Ricin_VPS13D"
                     /note="ricin B-type lectin domain, beta-trefoil fold,
                     found in vacuolar protein sorting-associated protein 13D
                     (VPS13D) and similar proteins; cd23453"
                     /db_xref="CDD:467331"
     Site            order(3221..3222,3223,3229,3231,3234,3239..3240)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467331"
     Site            order(3273..3276,3288,3290,3293,3299..3300)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467331"
     Site            order(3320..3323,3344,3346,3362..3363)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467331"
     Region          <3422..3910
                     /region_name="MRS6"
                     /note="Vacuolar protein sorting-associated protein
                     [Intracellular trafficking and secretion]; COG5043"
                     /db_xref="CDD:227376"
     CDS             1..3997
                     /gene="Vps13D"
                     /coded_by="XM_034334409.2:46..12039"
                     /db_xref="GeneID:117608798"
ORIGIN      
        1 mleglvawvl nnylgkyven lntdqlsial lsgeaelenl plkrealrhi glpieikagf
       61 igkvrlqvpv rqirtaswvi vieqlylvag pisleefdve aeeqaileyk lsrldslear
      121 wradtehdpg yyatsysswv nygtslvtni ienlqlnikd vhiryedgit lsdgialgit
      181 igaltaqscd aswipgstsw nltdasfklm eldklsiywn hlktneffgs lnlgdlaiam
      241 seprniikky vlspvnarah ikrdrserpl rstskprfiv dlllddvplc ltdvqyeqiv
      301 kcikglkdid rrkqqrrcrp tvsvssspke wwkyaarchl gkdtlkpkps wedilrrgke
      361 nilyvesytk llgtptsilp sdtkqlkekv eqersfdelr vlreiamrra rppkpvqnsd
      421 vnvpqprgml eqwfpqwwgw yskapnintq sssstfdgel ldvladtmdd dtllrrdtvf
      481 gqfnftlskg avslcttknd nekavielqf ervnlsyesr prsgshkfsi slgalylhdy
      541 ltenstfpil vqpqlifpat srirsstekl tnqlpqhlfe mtyekrplhl tadhslhvns
      601 rsldvvynpa vihwlidfmc kphrsssnqk lqamkrrtrr qliknweqil dadivyrssw
      661 dlqfnisapq illvenfvds naavvvvdfg klhlsnnvqn neviikpgsg ttsddederf
      721 etpcstppgs qengsiqdfq tisetalhqk lydhysidlv dlqilvgkvn dnwkhirtrg
      781 msslhvlerf nislkierrv fvssdpnlps vtvsgnlprl vvhvneqkve airlmysllt
      841 sfskstgiqq advinvepes pkkeesldks lshalmvqfm idqmtfelqs rgrsvaelqv
      901 sgvraafskr aadisvslsv hglllvdalq efgpdfellv ashkhvgmds isgslrdsep
      961 tspvspvspg spdptvprrl tspialtnal snlankaknp qsprnnslid lekmdseali
     1021 vvelmlvyda leilrvaniq fnnldiianq etivelmgff rrifpsrklr pgiierqesl
     1081 snqstepkvs tstritfdfh rlnvlllrav mqdnhlvgqk iatatmcdar iqatlavnas
     1141 isgslgglqv ldqtstgkth qriisvgrdp iadppqhpfe hftgltdqpe alrfsankct
     1201 klcfpqqdgt gtlidlavri gsvwythaph llselrscad efkqylsnla rqisaaatdm
     1261 aiglvnaedw sipprrrlps lsvdldnsgt lslkldvlld spvlviprss hsrqvfvahl
     1321 gtmslqheph pgsmakhkvt levrdmnlys leisanigka psnlplraeq mysckesgkp
     1381 ilhdtvlkvv vekettlaqs psfffdgefs nnpivqvhgv vmtplkvsls rgqyeqlldt
     1441 inrlfsmpvs vpvvqklqtt nkvpnnysdk ldlsikvlfe lptlsmelkq gpqeqplvel
     1501 smrdfvakye klqrnestiq valrsllmed llcpigsrhr cmmqssaptr aklpagvsks
     1561 cpnfafvqqv rsefgrgslp drletdtlyg tipahwrrkp vslihtpntp ppspgastse
     1621 enlvllsisi iepdcdqqtk dqfhqkivtv dfncldlvis veswmvvfdf fgfagpsrvn
     1681 pkvtmhqtsi dvdqldkvdn lplrseteie vrsltlvliq sereiakanv snasmhilka
     1741 ngktkisgsl gsmslldltp hgrfyrerfl ssgrkalnfq yssyvdtekr pydaqlklem
     1801 savhyvhtqr fvaelqaffg hfsqlwtima nlragvgavi dtnkrsmrls lqlqagapvi
     1861 llpvssrsde lilldlgelt ahnkfaesev vdkcmldvmf lelvnmdvya akrvncddqd
     1921 dkmdtlvvgg fhikklgssl ltemchlklr iernldtyis reipdlsihg tlstmdcald
     1981 paqymlirgl lsynigenle dlrqfmqdld qtaeysivsv dnrgkiwkks citlelvnvt
     2041 vklhpnhnia alacvnfiks rltldslsdg sqdidlvsqe iliidtrfqd epvnkqsnvf
     2101 tsilqplrns nnenrvqaev hhrrrktnaa ttilvhsmrl taildwweav rdflmlnsle
     2161 prpiigiaqt atesnqenen nastaiplel kinitdselv ivedtsvrdt navilkstav
     2221 lsyrsapheg ekplscnlsh celfscilgl eeetalsiid pvgasldltq dkcleiqlqp
     2281 lcvrlsyhdv tmfsrmlksl pkqtllakqr ssealtsaem qvqkltalgf ndadcraald
     2341 lcngqldeaa lwltqnavpd snvntnnesv fhtvelqtpc ariciiddcr dadvplleis
     2401 lldlnlkqac mgpglvsttf stdyynrvls gwepfvepwk atvhweqtlt nslnskrlqm
     2461 svdsqdsvdv nitstlveli elvknnwmqd fystsnkdvn vnkatgngta yrrrmpfipf
     2521 alknetgckl wfktiiatad dtkdigqnfk sknvfqkddt wlevvpgdti pftfegrgkl
     2581 rhhathtvrr hqitvlidgw rpvdpvtvdr vgiyfrhana nfttsqlrmf tpkarivfav
     2641 elegsarklv tvrsalqisn klkhpveikm ekslnqfghl slsvndrilq vpalstvsvp
     2701 lthtgiqmcl kplnlntlfq yctetidwtv vkkpleiien ritcranhnn ifrmcvavmr
     2761 dnyppdtsqi lpghtitvma pltltnllph ellfevgtes grilpggsad lhavnveeql
     2821 eitiqldgyp gpgmmilpph tnsftarlrl tdspgrilyl haavniekgs gtqinitapy
     2881 wiinktglpl vfrqegvgae aagqfeehek armvepllfs fsdeeasrtl svrvgtavhp
     2941 qgipqwsqhf hlqpgiqvhr lrislrdgrp divylisvat rtargkyrat tvvtlspryq
     3001 lhnkssftle laqkcfttti shpdaqatyi rtmpdchmpf hwprldkdll lcvriinvkd
     3061 cmwsggirld dnysltinir daagrmhflr vdvvlqesty fivftdadtm pppirvdnfs
     3121 evplivnqvt vhdslqwtvr phssvpyald epiqtaglvl tapggvtaty dlnmlgesrg
     3181 ltyenfiyia fagtfktdcd lgtgeggldp ldvetqrlvl eagpggwval rrkqygarsq
     3241 lwrmtgdgql qhegsspprd khsktpetvl vldiagtapq pftycalalr kpdprrrstq
     3301 twrftddgrl ccahknmcvq skdgfmglhe ggsvarwqmw seavlgpqth sspppieqrv
     3361 grqklrpgsg flsirvttdg ptrvlqvldi kerkktfall eerdwshiav shrptqiytd
     3421 aeklnsrele lkvnliaglg lsivsrrpve ellfarlagi slelvqtpan itldlsvedi
     3481 qidnqlfeaq cssvlyvtra arvesehrpa ihvvaeklks rsqnaeiykh vivtikplci
     3541 hleeklilkl aafigagrse levpvdendf kaqrfisevs aahakryyfg alkivpsqvk
     3601 lsvltttklp rhlqaikrql gltlikfeda tielepfikk hpfestqflv hsivkhykde
     3661 lkwqaavilg svdflgnplg fvndvtegvs gliyegsvkt lvknvthgis nsaakvtesl
     3721 sdglgrvimd erheearqri rantagssdh lvaglkgfgf gllggvtsif kqtydgaane
     3781 gfpgffagfg kgvvgtitkp vvgvldlate tatavretsr sahrtipkrd ryprvasgpa
     3841 gllppynrqq aegqeflyii ndhnyselfv ayeclrtgte nlrvlvsndr vrvisggtkg
     3901 vvtevsladl lycqpmhkve sndvtlyyie lisrsdstit vnvdgpellr rpkvrcdnee
     3961 vakrvsqqin yakgmheers ltltssdnmi ddvqyyk
//
LOCUS       XP_034190301            3988 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 13D isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190301
VERSION     XP_034190301.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334410.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190301.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3988
                     /product="vacuolar protein sorting 13D isoform X2"
                     /calculated_mol_wt=446678
     Region          1..>935
                     /region_name="MRS6"
                     /note="Vacuolar protein sorting-associated protein
                     [Intracellular trafficking and secretion]; COG5043"
                     /db_xref="CDD:227376"
     Region          <1611..>2748
                     /region_name="MRS6"
                     /note="Vacuolar protein sorting-associated protein
                     [Intracellular trafficking and secretion]; COG5043"
                     /db_xref="CDD:227376"
     Region          2874..3152
                     /region_name="SHR-BD"
                     /note="SHR-binding domain of vacuolar-sorting associated
                     protein 13; pfam06650"
                     /db_xref="CDD:461974"
     Region          3212..3361
                     /region_name="beta-trefoil_Ricin_VPS13D"
                     /note="ricin B-type lectin domain, beta-trefoil fold,
                     found in vacuolar protein sorting-associated protein 13D
                     (VPS13D) and similar proteins; cd23453"
                     /db_xref="CDD:467331"
     Site            order(3221..3222,3223,3229,3231,3234,3239..3240)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467331"
     Site            order(3273..3276,3288,3290,3293,3299..3300)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467331"
     Site            order(3320..3323,3335,3337,3353..3354)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467331"
     Region          <3413..3901
                     /region_name="MRS6"
                     /note="Vacuolar protein sorting-associated protein
                     [Intracellular trafficking and secretion]; COG5043"
                     /db_xref="CDD:227376"
     CDS             1..3988
                     /gene="Vps13D"
                     /coded_by="XM_034334410.2:46..12012"
                     /db_xref="GeneID:117608798"
ORIGIN      
        1 mleglvawvl nnylgkyven lntdqlsial lsgeaelenl plkrealrhi glpieikagf
       61 igkvrlqvpv rqirtaswvi vieqlylvag pisleefdve aeeqaileyk lsrldslear
      121 wradtehdpg yyatsysswv nygtslvtni ienlqlnikd vhiryedgit lsdgialgit
      181 igaltaqscd aswipgstsw nltdasfklm eldklsiywn hlktneffgs lnlgdlaiam
      241 seprniikky vlspvnarah ikrdrserpl rstskprfiv dlllddvplc ltdvqyeqiv
      301 kcikglkdid rrkqqrrcrp tvsvssspke wwkyaarchl gkdtlkpkps wedilrrgke
      361 nilyvesytk llgtptsilp sdtkqlkekv eqersfdelr vlreiamrra rppkpvqnsd
      421 vnvpqprgml eqwfpqwwgw yskapnintq sssstfdgel ldvladtmdd dtllrrdtvf
      481 gqfnftlskg avslcttknd nekavielqf ervnlsyesr prsgshkfsi slgalylhdy
      541 ltenstfpil vqpqlifpat srirsstekl tnqlpqhlfe mtyekrplhl tadhslhvns
      601 rsldvvynpa vihwlidfmc kphrsssnqk lqamkrrtrr qliknweqil dadivyrssw
      661 dlqfnisapq illvenfvds naavvvvdfg klhlsnnvqn neviikpgsg ttsddederf
      721 etpcstppgs qengsiqdfq tisetalhqk lydhysidlv dlqilvgkvn dnwkhirtrg
      781 msslhvlerf nislkierrv fvssdpnlps vtvsgnlprl vvhvneqkve airlmysllt
      841 sfskstgiqq advinvepes pkkeesldks lshalmvqfm idqmtfelqs rgrsvaelqv
      901 sgvraafskr aadisvslsv hglllvdalq efgpdfellv ashkhvgmds isgslrdsep
      961 tspvspvspg spdptvprrl tspialtnal snlankaknp qsprnnslid lekmdseali
     1021 vvelmlvyda leilrvaniq fnnldiianq etivelmgff rrifpsrklr pgiierqesl
     1081 snqstepkvs tstritfdfh rlnvlllrav mqdnhlvgqk iatatmcdar iqatlavnas
     1141 isgslgglqv ldqtstgkth qriisvgrdp iadppqhpfe hftgltdqpe alrfsankct
     1201 klcfpqqdgt gtlidlavri gsvwythaph llselrscad efkqylsnla rqisaaatdm
     1261 aiglvnaedw sipprrrlps lsvdldnsgt lslkldvlld spvlviprss hsrqvfvahl
     1321 gtmslqheph pgsmakhkvt levrdmnlys leisanigka psnlplraeq mysckesgkp
     1381 ilhdtvlkvv vekettlaqs psfffdgefs nnpivqvhgv vmtplkvsls rgqyeqlldt
     1441 inrlfsmpvs vpvvqklqtt nkvpnnysdk ldlsikvlfe lptlsmelkq gpqeqplvel
     1501 smrdfvakye klqrnestiq valrsllmed llcpigsrhr cmmqssaptr aklpagvsks
     1561 cpnfafvqqv rsefgrgslp drletdtlyg tipahwrrkp vslihtpntp ppspgastse
     1621 enlvllsisi iepdcdqqtk dqfhqkivtv dfncldlvis veswmvvfdf fgfagpsrvn
     1681 pkvtmhqtsi dvdqldkvdn lplrseteie vrsltlvliq sereiakanv snasmhilka
     1741 ngktkisgsl gsmslldltp hgrfyrerfl ssgrkalnfq yssyvdtekr pydaqlklem
     1801 savhyvhtqr fvaelqaffg hfsqlwtima nlragvgavi dtnkrsmrls lqlqagapvi
     1861 llpvssrsde lilldlgelt ahnkfaesev vdkcmldvmf lelvnmdvya akrvncddqd
     1921 dkmdtlvvgg fhikklgssl ltemchlklr iernldtyis reipdlsihg tlstmdcald
     1981 paqymlirgl lsynigenle dlrqfmqdld qtaeysivsv dnrgkiwkks citlelvnvt
     2041 vklhpnhnia alacvnfiks rltldslsdg sqdidlvsqe iliidtrfqd epvnkqsnvf
     2101 tsilqplrns nnenrvqaev hhrrrktnaa ttilvhsmrl taildwweav rdflmlnsle
     2161 prpiigiaqt atesnqenen nastaiplel kinitdselv ivedtsvrdt navilkstav
     2221 lsyrsapheg ekplscnlsh celfscilgl eeetalsiid pvgasldltq dkcleiqlqp
     2281 lcvrlsyhdv tmfsrmlksl pkqtllakqr ssealtsaem qvqkltalgf ndadcraald
     2341 lcngqldeaa lwltqnavpd snvntnnesv fhtvelqtpc ariciiddcr dadvplleis
     2401 lldlnlkqac mgpglvsttf stdyynrvls gwepfvepwk atvhweqtlt nslnskrlqm
     2461 svdsqdsvdv nitstlveli elvknnwmqd fystsnkdvn vnkatgngta yrrrmpfipf
     2521 alknetgckl wfktiiatad dtkdigqnfk sknvfqkddt wlevvpgdti pftfegrgkl
     2581 rhhathtvrr hqitvlidgw rpvdpvtvdr vgiyfrhana nfttsqlrmf tpkarivfav
     2641 elegsarklv tvrsalqisn klkhpveikm ekslnqfghl slsvndrilq vpalstvsvp
     2701 lthtgiqmcl kplnlntlfq yctetidwtv vkkpleiien ritcranhnn ifrmcvavmr
     2761 dnyppdtsqi lpghtitvma pltltnllph ellfevgtes grilpggsad lhavnveeql
     2821 eitiqldgyp gpgmmilpph tnsftarlrl tdspgrilyl haavniekgs gtqinitapy
     2881 wiinktglpl vfrqegvgae aagqfeehek armvepllfs fsdeeasrtl svrvgtavhp
     2941 qgipqwsqhf hlqpgiqvhr lrislrdgrp divylisvat rtargkyrat tvvtlspryq
     3001 lhnkssftle laqkcfttti shpdaqatyi rtmpdchmpf hwprldkdll lcvriinvkd
     3061 cmwsggirld dnysltinir daagrmhflr vdvvlqesty fivftdadtm pppirvdnfs
     3121 evplivnqvt vhdslqwtvr phssvpyald epiqtaglvl tapggvtaty dlnmlgesrg
     3181 ltyenfiyia fagtfktdcd lgtgeggldp ldvetqrlvl eagpggwval rrkqygarsq
     3241 lwrmtgdgql qhegsspprd khsktpetvl vldiagtapq pftycalalr kpdprrrstq
     3301 twrftddgrl ccahknmcvq skdgfmglhe gseavlgpqt hsspppieqr vgrqklrpgs
     3361 gflsirvttd gptrvlqvld ikerkktfal leerdwshia vshrptqiyt daeklnsrel
     3421 elkvnliagl glsivsrrpv eellfarlag islelvqtpa nitldlsved iqidnqlfea
     3481 qcssvlyvtr aarvesehrp aihvvaeklk srsqnaeiyk hvivtikplc ihleeklilk
     3541 laafigagrs elevpvdend fkaqrfisev saahakryyf galkivpsqv klsvltttkl
     3601 prhlqaikrq lgltlikfed atielepfik khpfestqfl vhsivkhykd elkwqaavil
     3661 gsvdflgnpl gfvndvtegv sgliyegsvk tlvknvthgi snsaakvtes lsdglgrvim
     3721 derheearqr irantagssd hlvaglkgfg fgllggvtsi fkqtydgaan egfpgffagf
     3781 gkgvvgtitk pvvgvldlat etatavrets rsahrtipkr dryprvasgp agllppynrq
     3841 qaegqeflyi indhnyself vayeclrtgt enlrvlvsnd rvrvisggtk gvvtevslad
     3901 llycqpmhkv esndvtlyyi elisrsdsti tvnvdgpell rrpkvrcdne evakrvsqqi
     3961 nyakgmheer sltltssdnm iddvqyyk
//
LOCUS       XP_034190302            3921 aa            linear   INV 03-SEP-2025
DEFINITION  hemolectin [Osmia lignaria lignaria].
ACCESSION   XP_034190302
VERSION     XP_034190302.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334411.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190302.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3921
                     /product="hemolectin"
                     /calculated_mol_wt=437256
     Region          393..550
                     /region_name="VWD"
                     /note="von Willebrand factor (vWF) type D domain;
                     smart00216"
                     /db_xref="CDD:214566"
     Region          595..665
                     /region_name="C8"
                     /note="C8 domain; pfam08742"
                     /db_xref="CDD:462584"
     Region          669..722
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(678,682,695,699)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     Region          724..>770
                     /region_name="VWC_out"
                     /note="von Willebrand factor (vWF) type C domain;
                     smart00215"
                     /db_xref="CDD:214565"
     Region          751..919
                     /region_name="VWD"
                     /note="von Willebrand factor (vWF) type D domain;
                     smart00216"
                     /db_xref="CDD:214566"
     Region          963..>1007
                     /region_name="C8"
                     /note="C8 domain; pfam08742"
                     /db_xref="CDD:462584"
     Region          1034..1088
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(1043,1047,1060,1064)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     Region          1153..1201
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(1155,1159,1172,1176)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     Region          1230..1387
                     /region_name="VWD"
                     /note="von Willebrand factor (vWF) type D domain;
                     smart00216"
                     /db_xref="CDD:214566"
     Region          1426..1498
                     /region_name="C8"
                     /note="This domain contains 8 conserved cysteine residues;
                     smart00832"
                     /db_xref="CDD:214843"
     Region          1627..1716
                     /region_name="Mucin2_WxxW"
                     /note="Mucin-2 protein WxxW repeating region; pfam13330"
                     /db_xref="CDD:463846"
     Region          1745..1788
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     Region          2001..2128
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Region          2184..2296
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Region          2607..2758
                     /region_name="VWD"
                     /note="von Willebrand factor type D domain; pfam00094"
                     /db_xref="CDD:459671"
     Region          2795..2867
                     /region_name="C8"
                     /note="C8 domain; pfam08742"
                     /db_xref="CDD:462584"
     Region          2871..2928
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(2880,2885,2898,2905)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     Region          2937..3094
                     /region_name="VWD"
                     /note="von Willebrand factor type D domain; pfam00094"
                     /db_xref="CDD:459671"
     Region          3135..3203
                     /region_name="C8"
                     /note="This domain contains 8 conserved cysteine residues;
                     smart00832"
                     /db_xref="CDD:214843"
     Region          3206..3261
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(3215,3219,3234,3238)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     Region          3265..3323
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     Region          <3843..3904
                     /region_name="GHB_like"
                     /note="Glycoprotein hormone beta chain homologues;
                     cl21545"
                     /db_xref="CDD:473907"
     CDS             1..3921
                     /gene="Hml"
                     /coded_by="XM_034334411.2:69..11834"
                     /db_xref="GeneID:117608799"
ORIGIN      
        1 mmlwkifvnv aviiilisss ivasdpadss rvgdttisln gdavpdyvgv krtksrrkpl
       61 faggcianpe vpvngrircs mdsgciatce qnykfpngvn qlaiicidkq wrivgtdwns
      121 mphcepicmp ecmnsgvcva phqcncpqdf tgpqcqfenk pclnypppvl nshklcnsks
      181 ctiscmknft fpdgsavtnl ickdgnwept kedwvsvpdc epvcdppcqn ggiclpsnlc
      241 qcpqaykgsq cqysadacng eklgfnggff cssvddsysc tlncpagief efppataytc
      301 sydtgvfhpq pipqckysen mnvislgtty nsyvretnhs wtyqdifssn mnqsplvktt
      361 ydkdfynnhe snmtsntmlf nsldnvllie ekrpvpetcf twggvnyktf ddtvfnfese
      421 csyilvqeaq nrlftiiven sprckvqdcf kiikifvqdk eyilsrneag vpefrtpnkl
      481 lpipaqlsyl rvemsahfiv vtldslgvql kwdgalmlqv eafenmwnkt tglcgnmngn
      541 kkddliskng eqmksiaafa sswrtekige tcdeypdtrh acesdpvntr eailfctkll
      601 sdrrfqacss tisiselqka clwdfcactr gdrrecacdt mnvyvrqcah kkiaslsgwr
      661 nddtcpmmcn ngrvymscgp ktesscwtgv erklnvedce egcfcpegtv ahegrcvypd
      721 ecpcrlrgkl fqpgksvqkd cntctcssgk wlctqvqcga rcavvgdphy ttfdgkhydf
      781 mgkckyylmk dddysiesen vpcsgaisen mglvpsdaps ctktvtinyq dvsvklkqhr
      841 qvlingddlt vyptvvkgvr irvassiflv ihlpndleiw wdgitrvyin vppqfhgktm
      901 glcgtfsenq kddfitpegd tenmaisfan kwktddscps lpekepdhpc dldpqkrdta
      961 kqycsylysd ifagchwhvd pdtfhkdclf dmcsckveve sclcpmlaay akecaaagik
     1021 lmwryeinec rihcpgnqvy qicgnsctrs cgdissyqdc kqecvegcnc pegetldvhg
     1081 ncipigqcpc tyqglefnag hkeirpgnka qelctcaggi wncqeatpde ikeypavkel
     1141 lascvaskhl evtdcapaep rtcrnmhkpv qkssvcksgc acklgyvlde pggncikeqs
     1201 cpchhggqsy kegsviqdec ntcecmngtw kctdrtcagv ctawgdshyk tfdgkiynfq
     1261 gmcdyllvkg slskvdtfdv siqnvpcgtt gvacsksvtl tvgsnespes ivftrgkelp
     1321 sgnfkrivmr ttdlfvfvdv pdmgltlqwd rgtrvyvkld pkwkgrtkgl cgdyndnsed
     1381 dfktpsggis evsanlfgds wkkndlcpep kdikdpceqh pernlwsvqk cgilkspvfq
     1441 pchseveven yirncifdtc gcdaggdcec lctalaayah ecnakgvpik wrsqelcpiq
     1501 cdekcssysp cittcpretc dnlmtlkdqs hlcsqdtcve gcstkscsen qvytndsyte
     1561 cvpketcksf cieiegvtyy egdnvksddc qtcycsrgkv lckgepcttt aastvpleep
     1621 qkcvdgwtaw inqdpgikgk kikdieplpt lldlasiegs avcdrshmie ircrsvkghl
     1681 tpketgldve cnlehglycq tqpkhpcidf eisvlcqcse ttteklgiss tvnamfeecn
     1741 meqpykphpm dcrlfyqcsp gpngnefvek scgenmfynp qiqvcdwpas viqirpecsv
     1801 eqtstktewt kyttlgknii ttkackeget wsncaidcnk vcdyyhyill kegtcngnsd
     1861 cvpgcipeer pvcppnkfwr dsmtcveead ctckshdgqs ilpsavikes eceicqclnn
     1921 yytcdrslcl kstekpityp qtettttpts plfenekstt rspvnevtiv vpstvsppay
     1981 cesnnfvpli qylsdqvsfe assskgpafq aensvlneda gywepeypgt dqwldikfqk
     2041 pepiygiilq gsgvedkfvt syqvlfseng hsfshvlddk kqprifsgpv nkykpveqkf
     2101 hepieakvvr inplswhngi airvellgcq elittiplte tvpvtvitdk iispvcdepm
     2161 gvdngliype qlstsssstd llpnlklssp svwhpkldnp hqfieidfle prnltgiatk
     2221 ggegtwttvy kvfysndghq wnpvmdtngy eqeflgnfds etvkknyfdr plsarylklq
     2281 pikwhehigl kfevlgcflp yppittttea mestprtiek flvcnickgi mvedqitckc
     2341 getywwngdt cvteqecpcv vghisynvga mwvsedcqec vctlggisfc qpkkceacne
     2401 pgmrpvinel cncvckpcpp gtrhcptsdv cieenlwcng vqdcpddekd cpqvvettpt
     2461 lkqttestpi istsvaptvc qdpvcppgyr tvlkssmksq rytnshgmkg gakslhrhsl
     2521 rmkglrksmq hskyneekes esgtecaqft cvpnkpppil dkntphtcpe vscppgyavv
     2581 yekmsmyklq kcpkytckpp apeevvcnvt grtfttfdkl eykydicnhi lardmfankw
     2641 yitlekscda hnnqcarvla itldedvivl ypdmhvdinq ysftpnqvar fgdrfpsfki
     2701 ssigdityvv snyygfwviw dsnsnvklgv stklagkvdg lcgyfdgysm ndrqlpdgnq
     2761 arstvefgds wmmegvpecd pqvcphyvqe eswkicnivk daslaecsni vdlekfishc
     2821 mestcnclrg nhtyndcrcr lltsfvtecq agnlnadlst wrsihdcpvs cptplvhkdc
     2881 frnkcetscd nlqqidpcpm mqgvcfsgcf cpegtvrngd qcvppmqckd ctcewlgnsk
     2941 fisfdrkniq fdgnctyvls rnieskrapq dyayqvlvsn gicntgtcte aiiilyqehv
     3001 vqikegvpsq efevqvdgsk isdfpynasw lnleqwpsek lrllipsiql eiisyqpnfa
     3061 ftltlpshif ggtmeglcgn cnedtdddlk rqdgeitdnt qdfgtswlvt esprginldm
     3121 gactsnnqsk cilppadqdp crtllntmdf gqchnlvdpm pylmacqdnl csgggycdsf
     3181 eayarkcqtm gvcliwrsse icpftcpphl eyrpcgpack etcdtineit nalcptnfee
     3241 gcfcplnfis hndtcipkek cflcdeeghv egdtwspdvc tqctcekkti ncqkteclav
     3301 dticeedmtp vvvngteeac cpkyyclpkm vttlppvcte etqmpecgyg qmmkvsigad
     3361 gcqkficecl ppsecpflne vsvqveqlqp gfvqvtntsg ccprfmtice letcppapvc
     3421 peyyelkskv esnaccakyd cvpakdlcly nvtnkqiems ehvvarklge qwmedkctsc
     3481 icehseigpk pkcftteclk avdhpdisdf vleevvtedk ccpsfertac kdgermynvg
     3541 eswqpnpeds ctlmecikng dgvqkqakvq ecstvcdlgf kyrptdnksi nccgrcipva
     3601 cvvgdelknv geewysvdfc tkyscvstnd tvyvesvtek cleidpvqea efeiqkqyip
     3661 gqccpqfvkt gcryndiiyk pgdkwksted ncvtevcald sngtkykeve scskkcslgw
     3721 tyepqdgecc gkckqsycvv gdmlyepgat wssadnctsy tcmeqgdqls itsnsavcpd
     3781 ishcpvtsvy tdncckmcnl tsynqkvnlc aadvldaqst vgmfnvqhri hgvcknlqpi
     3841 egitecrgac estsyfdidn wsqvvncqcc qptkyrglii eltcedskvl kkqvpqpvsc
     3901 tcsacssnes gykgrkggvk g
//
LOCUS       XP_034190303            3098 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608800 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190303
VERSION     XP_034190303.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334412.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190303.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3098
                     /product="uncharacterized protein LOC117608800 isoform X1"
                     /calculated_mol_wt=358701
     Region          <9..261
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          141..>870
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          828..1725
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1596..2407
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <2125..2916
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2939..3019
                     /region_name="PACT_coil_coil"
                     /note="Pericentrin-AKAP-450 domain of centrosomal
                     targeting protein; pfam10495"
                     /db_xref="CDD:463115"
     CDS             1..3098
                     /gene="LOC117608800"
                     /coded_by="XM_034334412.2:206..9502"
                     /db_xref="GeneID:117608800"
ORIGIN      
        1 mskatddder rrrsleagre mlekykvera kvqgtshsqm geasdeeslh ndkslgrres
       61 yahegissrd vtqssvsmse geadgdlegl agriaqleel lqgkeaivea lnaeidhlra
      121 etsspnssqs qnssihsrdv islyhiklqe fekavnqrdn lieeltwsvq halsardnla
      181 aqvnslnaml enanavsnkn lqekidtlen tlsdqkstiq klngqliqiq ehvqtlemek
      241 etrnaeindy klqignlneq irigaadknl niaetleqqk qyearvdkik qdmqhilkkf
      301 taetnintar hqqevkdlva kheteivniq ekyeerlkql keenkifadr lnkelpdles
      361 rhakelsifq tqlahykktv ealklelvnr sesqqtaqae lneyksklne tkvqsertrh
      421 vqnlehqkek emlteqiklh klqleemtsk yiatsavles kesiersleq altsaatlke
      481 eneslkfkld dlssrysaaq sliensqahe rtlsnkiydl ekslsrlsgi nastlselne
      541 ttyqtfdeva iqyqltkqrl eekaelekll vqrienlekd vrksreeleq anlvkksyek
      601 qlkdmkntcd kykselsslr nesgqssfql deekklssqk hnvadllrkt eedqqeikkl
      661 kaileqkete lseslkkmyd lserlkksee eteqlkhgla sawaqcaeve eklnqtlals
      721 dskldvsvps ssyssalkqf rlgrvanesv ntshdqsmdt nkilydkdeq idsesrarlq
      781 vkltsvseen erlskelecl mekqanyeei knklkhysml aenlsteker tneenktlkk
      841 klenlhslqe sinqmrveke slrkeieala cvhdeqitai kaetaseirk vqslmlglka
      901 gttelsdlki elemrhakem eelrtyfeqk cllmekqyse eifsqqskkm sdndseiadl
      961 teglyfgagg dclnvsnisg kssrlgspit ddnlkqnsns fnnqsrsele yktnvkalqq
     1021 elqnkiieaq emklnyektl eeqksvhere lydikakerh dflrsvvnqh cqtewdagal
     1081 engeltqlra aydhqleeqi alakldivna lqeqiqalla vesdsednwp pellklrdkl
     1141 tnnakremqq lkethavelq rlkeeysrnv armidrhqee lnkfrldgac dncgrielns
     1201 itdnkileer nslnktcatl kslieeliky fviceeevnn tlitevlkrq lsdslsnvkt
     1261 qkmdeldask rslesdtlns samrvqrvhf apqstgivsi insntetlpt ileededite
     1321 klkqelnncv rrlksesaei lgisfatiea qrssfskdtw mdkvneelnl klrqaealim
     1381 gyqeeaeqlk vtildlqrkl isaeskkeii tegygendei gtditledfs qlhekarhvl
     1441 snggadcsel lqlleglcrh ndkltedakr eredlqqqqv pleptpppyi hrvccrkida
     1501 adkqlkatrr fldeqasere aerdeaarqi hllqeqlker erekerdlri sseqsqlspe
     1561 aasevhviqa saskaavevl esqmremssm msdteakkte teselkaaid kiwvlreiit
     1621 eleqqlqikt ekeeslqmqi nqletviaaq tknqhelvqe ldavkmgses khlnehinql
     1681 qeelrkhkls seqfkvnssa lkqmktelrd mqnqldkrir dlesahmcss nlslsqpsed
     1741 vsireqidas rcptpddpts ppmlpldqll klkekmlkha raeevafkri kdlemqvtaf
     1801 knqneelqae qeilqqtase qlfqieamrg rleqhkqsap faqrqatsrl elqlheanak
     1861 fqslertiad kdlelkdvrs qldrvnqllq ekeaeianvv qvenatiqkl rehievleee
     1921 kkilqakvgv qehaqlelpr lidsmladkn eeidhlkeql skkekqldly sslnldetql
     1981 relvrqteak nsartlsdil sihseceetv eairglnpaq tlpnistfkv ptaavasktv
     2041 ddsvplmdlq vppldlgshs ysptsnqqsg midllhggsg sristsenhf snpssqttqe
     2101 stqmqesrrq ecnknessnv svkctqtsln etmkevedle nqlqaiqeel qiksmilekr
     2161 eseligmqki ynelqaefke tvetltrdkc fyqnqyelsq vsenkikedl qevenvlklr
     2221 tdeiqeqknk mqvnekiime lnqentklkk dveekeqeha rkynilwqek meelqnfraa
     2281 ilekditiet iqtrnieied enkqlyefkt kyqstkqeml ecqneiqrlt eglnnrdqii
     2341 rrleemarrt sysgtsspsn ekdqeihhlq eylkekdkvi rqmsddsksl hkaletiqnk
     2401 mkesgnviel rkklkderkl nselrnmvek lskelfdlks saqrsqddtd iedmvqreln
     2461 lsahldrqim naiesdpeek arrvenqvqy qeyqegrlmd lklklsqvnk ineelkklkd
     2521 dleiergmlk cqiaeyegri fqlksdltee skklekleee lssernlvrt lkiqiekehr
     2581 amqsghiqds elieflqnkl ktsldneakl rndlstlrqe hksleiqlsl mkdhvesqkt
     2641 delpkladll egerrkylsl menfekeerd naelkdtlkk lqmeksrfek qleveveeke
     2701 klisglalie gikdhlqtdl rrtkeelrar eeecdwlqkr iktmldaesr rqeqrtsehn
     2761 elkalrreis ntrevmmdle admkqskrel tesleremkl aealeklker etdllkklsa
     2821 gkeeerklkd tilelqhdfr tstrtesest tefksrytne ksvpvkyiqk ikelseinek
     2881 ymneigmihe klietreeke qlnrkvrlle aqvkrikdsd ngnlmgpnyt eklqhfygky
     2941 lradsrrkal tyqkryllti isgyqlseen tlsvlaqltk eqrtyttmgh srkspkirfk
     3001 navlvlisih rmkwlilrws sgkrigvktl lwnadqafaq vpkvamnhsp pvrerrttng
     3061 eggfdglale qyyqrlkniq qtlglamaes gsrpiipe
//
LOCUS       XP_034190304            3097 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608800 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190304
VERSION     XP_034190304.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334413.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190304.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3097
                     /product="uncharacterized protein LOC117608800 isoform X2"
                     /calculated_mol_wt=358573
     Region          <9..261
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          141..>870
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          828..1724
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1595..2406
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <2124..2915
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2938..3018
                     /region_name="PACT_coil_coil"
                     /note="Pericentrin-AKAP-450 domain of centrosomal
                     targeting protein; pfam10495"
                     /db_xref="CDD:463115"
     CDS             1..3097
                     /gene="LOC117608800"
                     /coded_by="XM_034334413.2:206..9499"
                     /db_xref="GeneID:117608800"
ORIGIN      
        1 mskatddder rrrsleagre mlekykvera kvqgtshsqm geasdeeslh ndkslgrres
       61 yahegissrd vtqssvsmse geadgdlegl agriaqleel lqgkeaivea lnaeidhlra
      121 etsspnssqs qnssihsrdv islyhiklqe fekavnqrdn lieeltwsvq halsardnla
      181 aqvnslnaml enanavsnkn lqekidtlen tlsdqkstiq klngqliqiq ehvqtlemek
      241 etrnaeindy klqignlneq irigaadknl niaetleqqk qyearvdkik qdmqhilkkf
      301 taetnintar hqqevkdlva kheteivniq ekyeerlkql keenkifadr lnkelpdles
      361 rhakelsifq tqlahykktv ealklelvnr sesqqtaqae lneyksklne tkvqsertrh
      421 vqnlehqkek emlteqiklh klqleemtsk yiatsavles kesiersleq altsaatlke
      481 eneslkfkld dlssrysaaq sliensqahe rtlsnkiydl ekslsrlsgi nastlselne
      541 ttyqtfdeva iqyqltkqrl eekaelekll vqrienlekd vrksreeleq anlvkksyek
      601 qlkdmkntcd kykselsslr nesgqssfql deekklssqk hnvadllrkt eedqqeikkl
      661 kaileqkete lseslkkmyd lserlkksee eteqlkhgla sawaqcaeve eklnqtlals
      721 dskldvsvps ssyssalkqf rlgrvanesv ntshdqsmdt nkilydkdeq idsesrarlq
      781 vkltsvseen erlskelecl mekqanyeei knklkhysml aenlsteker tneenktlkk
      841 klenlhslqe sinqmrveke slrkeieala cvhdeqitai kaetaseirk vqslmlglka
      901 gttelsdlki elemrhakem eelrtyfeqk cllmekqyse eifsqqskkm sdndseiadl
      961 teglyfgagg dclnvsnisg kssrlgspit ddnlkqnsns fnnqsrsele yktnvkalqq
     1021 elqnkiieaq emklnyektl eeqksvhere lydikakerh dflrsvvnqh cqtewdagal
     1081 engeltqlra aydhqleeqi alakldivna lqeqiqalla vesdsednwp pellklrdkl
     1141 tnnakremqq lkethavelq rlkeeysrnv armidrhqee lnkfrldgac dncgrielns
     1201 itdnkileer nslnktcatl kslieeliky fviceeevnn tlitevlkrq lsdslsnvkt
     1261 qkmdeldask rslesdtlns samrvqrvhf apqstgivsi insntetlpt ileededite
     1321 klkqelnncv rrlksesaei lgisfatiea qrssfskdtw mdkvneelnl klrqaealim
     1381 gyqeeaeqlk vtildlqrkl isaeskkeii tegygendei gtditledfs qlhekarhvl
     1441 snggadcsel lqlleglcrh ndkltedakr eredlqqqqv pleptpppyi hrvccrkida
     1501 adkqlkatrr fldeqasere aerdeaarqi hllqeqlker erekerdlri ssesqlspea
     1561 asevhviqas askaavevle sqmremssmm sdteakktet eselkaaidk iwvlreiite
     1621 leqqlqikte keeslqmqin qletviaaqt knqhelvqel davkmgsesk hlnehinqlq
     1681 eelrkhklss eqfkvnssal kqmktelrdm qnqldkrird lesahmcssn lslsqpsedv
     1741 sireqidasr cptpddptsp pmlpldqllk lkekmlkhar aeevafkrik dlemqvtafk
     1801 nqneelqaeq eilqqtaseq lfqieamrgr leqhkqsapf aqrqatsrle lqlheanakf
     1861 qslertiadk dlelkdvrsq ldrvnqllqe keaeianvvq venatiqklr ehievleeek
     1921 kilqakvgvq ehaqlelprl idsmladkne eidhlkeqls kkekqldlys slnldetqlr
     1981 elvrqteakn sartlsdils ihseceetve airglnpaqt lpnistfkvp taavasktvd
     2041 dsvplmdlqv ppldlgshsy sptsnqqsgm idllhggsgs ristsenhfs npssqttqes
     2101 tqmqesrrqe cnknessnvs vkctqtslne tmkevedlen qlqaiqeelq iksmilekre
     2161 seligmqkiy nelqaefket vetltrdkcf yqnqyelsqv senkikedlq evenvlklrt
     2221 deiqeqknkm qvnekiimel nqentklkkd veekeqehar kynilwqekm eelqnfraai
     2281 lekditieti qtrnieiede nkqlyefktk yqstkqemle cqneiqrlte glnnrdqiir
     2341 rleemarrts ysgtsspsne kdqeihhlqe ylkekdkvir qmsddskslh kaletiqnkm
     2401 kesgnvielr kklkderkln selrnmvekl skelfdlkss aqrsqddtdi edmvqrelnl
     2461 sahldrqimn aiesdpeeka rrvenqvqyq eyqegrlmdl klklsqvnki neelkklkdd
     2521 leiergmlkc qiaeyegrif qlksdltees kklekleeel ssernlvrtl kiqiekehra
     2581 mqsghiqdse lieflqnklk tsldneaklr ndlstlrqeh ksleiqlslm kdhvesqktd
     2641 elpkladlle gerrkylslm enfekeerdn aelkdtlkkl qmeksrfekq leveveekek
     2701 lisglalieg ikdhlqtdlr rtkeelrare eecdwlqkri ktmldaesrr qeqrtsehne
     2761 lkalrreisn trevmmdlea dmkqskrelt esleremkla ealeklkere tdllkklsag
     2821 keeerklkdt ilelqhdfrt strtesestt efksrytnek svpvkyiqki kelseineky
     2881 mneigmihek lietreekeq lnrkvrllea qvkrikdsdn gnlmgpnyte klqhfygkyl
     2941 radsrrkalt yqkrylltii sgyqlseent lsvlaqltke qrtyttmghs rkspkirfkn
     3001 avlvlisihr mkwlilrwss gkrigvktll wnadqafaqv pkvamnhspp vrerrttnge
     3061 ggfdglaleq yyqrlkniqq tlglamaesg srpiipe
//
LOCUS       XP_034190305            3097 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608800 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190305
VERSION     XP_034190305.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334414.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190305.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3097
                     /product="uncharacterized protein LOC117608800 isoform X3"
                     /calculated_mol_wt=358573
     Region          <9..261
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          141..>870
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          828..1724
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1595..2406
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <2124..2915
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2938..3018
                     /region_name="PACT_coil_coil"
                     /note="Pericentrin-AKAP-450 domain of centrosomal
                     targeting protein; pfam10495"
                     /db_xref="CDD:463115"
     CDS             1..3097
                     /gene="LOC117608800"
                     /coded_by="XM_034334414.2:206..9499"
                     /db_xref="GeneID:117608800"
ORIGIN      
        1 mskatddder rrrsleagre mlekykvera kvqgtshsqm geasdeeslh ndkslgrres
       61 yahegissrd vtqssvsmse geadgdlegl agriaqleel lqgkeaivea lnaeidhlra
      121 etsspnssqs qnssihsrdv islyhiklqe fekavnqrdn lieeltwsvq halsardnla
      181 aqvnslnaml enanavsnkn lqekidtlen tlsdqkstiq klngqliqiq ehvqtlemek
      241 etrnaeindy klqignlneq irigaadknl niaetleqqk qyearvdkik qdmqhilkkf
      301 taetnintar hqqevkdlva kheteivniq ekyeerlkql keenkifadr lnkelpdles
      361 rhakelsifq tqlahykktv ealklelvnr sesqqtaqae lneyksklne tkvqsertrh
      421 vqnlehqkek emlteqiklh klqleemtsk yiatsavles kesiersleq altsaatlke
      481 eneslkfkld dlssrysaaq sliensqahe rtlsnkiydl ekslsrlsgi nastlselne
      541 ttyqtfdeva iqyqltkqrl eekaelekll vqrienlekd vrksreeleq anlvkksyek
      601 qlkdmkntcd kykselsslr nesgqssfql deekklssqk hnvadllrkt eedqqeikkl
      661 kaileqkete lseslkkmyd lserlkksee eteqlkhgla sawaqcaeve eklnqtlals
      721 dskldvsvps ssyssalkqf rlgrvanesv ntshdqsmdt nkilydkdeq idsesrarlq
      781 vkltsvseen erlskelecl mekqanyeei knklkhysml aenlsteker tneenktlkk
      841 klenlhslqe sinqmrveke slrkeieala cvhdeqitai kaetaseirk vqslmlglka
      901 gttelsdlki elemrhakem eelrtyfeqk cllmekqyse eifsqqskkm sdndseiadl
      961 teglyfgagg dclnvsnisg kssrlgspit ddnlkqnsns fnnqsrsele yktnvkalqq
     1021 elqnkiieaq emklnyektl eeqksvhere lydikakerh dflrsvvnqh cqtewdagal
     1081 engeltqlra aydhqleeqi alakldivna lqeqiqalla vesdsednwp pellklrdkl
     1141 tnnakremqq lkethavelq rlkeeysrnv armidrhqee lnkfrldgac dncgrielns
     1201 itdnkileer nslnktcatl kslieeliky fviceeevnn tlitevlkrq lsdslsnvkt
     1261 qkmdeldask rslesdtlns samrvqrvhf apqstgivsi insntetlpt ileededite
     1321 klkqelnncv rrlksesaei lgisfatiea qrssfskdtw mdkvneelnl klrqaealim
     1381 gyqeeaeqlk vtildlqrkl isaeskkeii tegygendei gtditledfs qlhekarhvl
     1441 snggadcsel lqlleglcrh ndkltedakr eredlqqqvp leptpppyih rvccrkidaa
     1501 dkqlkatrrf ldeqaserea erdeaarqih llqeqlkere rekerdlris seqsqlspea
     1561 asevhviqas askaavevle sqmremssmm sdteakktet eselkaaidk iwvlreiite
     1621 leqqlqikte keeslqmqin qletviaaqt knqhelvqel davkmgsesk hlnehinqlq
     1681 eelrkhklss eqfkvnssal kqmktelrdm qnqldkrird lesahmcssn lslsqpsedv
     1741 sireqidasr cptpddptsp pmlpldqllk lkekmlkhar aeevafkrik dlemqvtafk
     1801 nqneelqaeq eilqqtaseq lfqieamrgr leqhkqsapf aqrqatsrle lqlheanakf
     1861 qslertiadk dlelkdvrsq ldrvnqllqe keaeianvvq venatiqklr ehievleeek
     1921 kilqakvgvq ehaqlelprl idsmladkne eidhlkeqls kkekqldlys slnldetqlr
     1981 elvrqteakn sartlsdils ihseceetve airglnpaqt lpnistfkvp taavasktvd
     2041 dsvplmdlqv ppldlgshsy sptsnqqsgm idllhggsgs ristsenhfs npssqttqes
     2101 tqmqesrrqe cnknessnvs vkctqtslne tmkevedlen qlqaiqeelq iksmilekre
     2161 seligmqkiy nelqaefket vetltrdkcf yqnqyelsqv senkikedlq evenvlklrt
     2221 deiqeqknkm qvnekiimel nqentklkkd veekeqehar kynilwqekm eelqnfraai
     2281 lekditieti qtrnieiede nkqlyefktk yqstkqemle cqneiqrlte glnnrdqiir
     2341 rleemarrts ysgtsspsne kdqeihhlqe ylkekdkvir qmsddskslh kaletiqnkm
     2401 kesgnvielr kklkderkln selrnmvekl skelfdlkss aqrsqddtdi edmvqrelnl
     2461 sahldrqimn aiesdpeeka rrvenqvqyq eyqegrlmdl klklsqvnki neelkklkdd
     2521 leiergmlkc qiaeyegrif qlksdltees kklekleeel ssernlvrtl kiqiekehra
     2581 mqsghiqdse lieflqnklk tsldneaklr ndlstlrqeh ksleiqlslm kdhvesqktd
     2641 elpkladlle gerrkylslm enfekeerdn aelkdtlkkl qmeksrfekq leveveekek
     2701 lisglalieg ikdhlqtdlr rtkeelrare eecdwlqkri ktmldaesrr qeqrtsehne
     2761 lkalrreisn trevmmdlea dmkqskrelt esleremkla ealeklkere tdllkklsag
     2821 keeerklkdt ilelqhdfrt strtesestt efksrytnek svpvkyiqki kelseineky
     2881 mneigmihek lietreekeq lnrkvrllea qvkrikdsdn gnlmgpnyte klqhfygkyl
     2941 radsrrkalt yqkrylltii sgyqlseent lsvlaqltke qrtyttmghs rkspkirfkn
     3001 avlvlisihr mkwlilrwss gkrigvktll wnadqafaqv pkvamnhspp vrerrttnge
     3061 ggfdglaleq yyqrlkniqq tlglamaesg srpiipe
//
LOCUS       XP_034190307            3079 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608800 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190307
VERSION     XP_034190307.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334416.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190307.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3079
                     /product="uncharacterized protein LOC117608800 isoform X4"
                     /calculated_mol_wt=356486
     Region          <9..261
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          141..>870
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          828..1706
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1577..2388
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <2106..2897
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2920..3000
                     /region_name="PACT_coil_coil"
                     /note="Pericentrin-AKAP-450 domain of centrosomal
                     targeting protein; pfam10495"
                     /db_xref="CDD:463115"
     CDS             1..3079
                     /gene="LOC117608800"
                     /coded_by="XM_034334416.2:206..9445"
                     /db_xref="GeneID:117608800"
ORIGIN      
        1 mskatddder rrrsleagre mlekykvera kvqgtshsqm geasdeeslh ndkslgrres
       61 yahegissrd vtqssvsmse geadgdlegl agriaqleel lqgkeaivea lnaeidhlra
      121 etsspnssqs qnssihsrdv islyhiklqe fekavnqrdn lieeltwsvq halsardnla
      181 aqvnslnaml enanavsnkn lqekidtlen tlsdqkstiq klngqliqiq ehvqtlemek
      241 etrnaeindy klqignlneq irigaadknl niaetleqqk qyearvdkik qdmqhilkkf
      301 taetnintar hqqevkdlva kheteivniq ekyeerlkql keenkifadr lnkelpdles
      361 rhakelsifq tqlahykktv ealklelvnr sesqqtaqae lneyksklne tkvqsertrh
      421 vqnlehqkek emlteqiklh klqleemtsk yiatsavles kesiersleq altsaatlke
      481 eneslkfkld dlssrysaaq sliensqahe rtlsnkiydl ekslsrlsgi nastlselne
      541 ttyqtfdeva iqyqltkqrl eekaelekll vqrienlekd vrksreeleq anlvkksyek
      601 qlkdmkntcd kykselsslr nesgqssfql deekklssqk hnvadllrkt eedqqeikkl
      661 kaileqkete lseslkkmyd lserlkksee eteqlkhgla sawaqcaeve eklnqtlals
      721 dskldvsvps ssyssalkqf rlgrvanesv ntshdqsmdt nkilydkdeq idsesrarlq
      781 vkltsvseen erlskelecl mekqanyeei knklkhysml aenlsteker tneenktlkk
      841 klenlhslqe sinqmrveke slrkeieala cvhdeqitai kaetaseirk vqslmlglka
      901 gttelsdlki elemrhakem eelrtyfeqk cllmekqyse eifsqqskkm sdndseiadl
      961 teglyfgagg dclnvsnisg kssrlgspit ddnlkqnsns fnnqsrsele yktnvkalqq
     1021 elqnkiieaq emklnyektl eeqksvhere lydikakerh dflrsvvnqh cqtewdagal
     1081 engeltqlra aydhqleeqi alakldivna lqeqiqalla vesdsednwp pellklrdkl
     1141 tnnakremqq lkethavelq rlkeeysrnv armidrhqee lnkfrldgac dncgrielns
     1201 itdnkileer nslnktcatl kslieeliky fviceeevnn tlitevlkrq lsdslsnvkt
     1261 qkmdeldask rslesdtlns samrvqrvhf apqstgivsi insntetlpt ileededite
     1321 klkqelnncv rrlksesaei lgisfatiea qrssfskdtw mdkvneelnl klrqaealim
     1381 gyqeeaeqlk vtildlqrkl isaeskkeii tegygendei gtditledfs qlhekarhvl
     1441 snggadcsel lqlleglcrh ndkltedakr eredlqqqid aadkqlkatr rfldeqaser
     1501 eaerdeaarq ihllqeqlke rerekerdlr isseqsqlsp eaasevhviq asaskaavev
     1561 lesqmremss mmsdteakkt eteselkaai dkiwvlreii teleqqlqik tekeeslqmq
     1621 inqletviaa qtknqhelvq eldavkmgse skhlnehinq lqeelrkhkl sseqfkvnss
     1681 alkqmktelr dmqnqldkri rdlesahmcs snlslsqpse dvsireqida srcptpddpt
     1741 sppmlpldql lklkekmlkh araeevafkr ikdlemqvta fknqneelqa eqeilqqtas
     1801 eqlfqieamr grleqhkqsa pfaqrqatsr lelqlheana kfqslertia dkdlelkdvr
     1861 sqldrvnqll qekeaeianv vqvenatiqk lrehievlee ekkilqakvg vqehaqlelp
     1921 rlidsmladk neeidhlkeq lskkekqldl ysslnldetq lrelvrqtea knsartlsdi
     1981 lsihseceet veairglnpa qtlpnistfk vptaavaskt vddsvplmdl qvppldlgsh
     2041 sysptsnqqs gmidllhggs gsristsenh fsnpssqttq estqmqesrr qecnknessn
     2101 vsvkctqtsl netmkevedl enqlqaiqee lqiksmilek reseligmqk iynelqaefk
     2161 etvetltrdk cfyqnqyels qvsenkiked lqevenvlkl rtdeiqeqkn kmqvnekiim
     2221 elnqentklk kdveekeqeh arkynilwqe kmeelqnfra ailekditie tiqtrnieie
     2281 denkqlyefk tkyqstkqem lecqneiqrl teglnnrdqi irrleemarr tsysgtssps
     2341 nekdqeihhl qeylkekdkv irqmsddsks lhkaletiqn kmkesgnvie lrkklkderk
     2401 lnselrnmve klskelfdlk ssaqrsqddt diedmvqrel nlsahldrqi mnaiesdpee
     2461 karrvenqvq yqeyqegrlm dlklklsqvn kineelkklk ddleiergml kcqiaeyegr
     2521 ifqlksdlte eskkleklee elssernlvr tlkiqiekeh ramqsghiqd selieflqnk
     2581 lktsldneak lrndlstlrq ehksleiqls lmkdhvesqk tdelpkladl legerrkyls
     2641 lmenfekeer dnaelkdtlk klqmeksrfe kqleveveek eklisglali egikdhlqtd
     2701 lrrtkeelra reeecdwlqk riktmldaes rrqeqrtseh nelkalrrei sntrevmmdl
     2761 eadmkqskre ltesleremk laealeklke retdllkkls agkeeerklk dtilelqhdf
     2821 rtstrteses ttefksrytn eksvpvkyiq kikelseine kymneigmih eklietreek
     2881 eqlnrkvrll eaqvkrikds dngnlmgpny teklqhfygk ylradsrrka ltyqkryllt
     2941 iisgyqlsee ntlsvlaqlt keqrtyttmg hsrkspkirf knavlvlisi hrmkwlilrw
     3001 ssgkrigvkt llwnadqafa qvpkvamnhs ppvrerrttn geggfdglal eqyyqrlkni
     3061 qqtlglamae sgsrpiipe
//
LOCUS       XP_034190308            3075 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608800 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190308
VERSION     XP_034190308.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334417.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190308.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3075
                     /product="uncharacterized protein LOC117608800 isoform X5"
                     /calculated_mol_wt=356410
     Region          <9..261
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          141..>870
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          828..1702
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1496..2384
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <2102..2893
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2916..2996
                     /region_name="PACT_coil_coil"
                     /note="Pericentrin-AKAP-450 domain of centrosomal
                     targeting protein; pfam10495"
                     /db_xref="CDD:463115"
     CDS             1..3075
                     /gene="LOC117608800"
                     /coded_by="XM_034334417.2:206..9433"
                     /db_xref="GeneID:117608800"
ORIGIN      
        1 mskatddder rrrsleagre mlekykvera kvqgtshsqm geasdeeslh ndkslgrres
       61 yahegissrd vtqssvsmse geadgdlegl agriaqleel lqgkeaivea lnaeidhlra
      121 etsspnssqs qnssihsrdv islyhiklqe fekavnqrdn lieeltwsvq halsardnla
      181 aqvnslnaml enanavsnkn lqekidtlen tlsdqkstiq klngqliqiq ehvqtlemek
      241 etrnaeindy klqignlneq irigaadknl niaetleqqk qyearvdkik qdmqhilkkf
      301 taetnintar hqqevkdlva kheteivniq ekyeerlkql keenkifadr lnkelpdles
      361 rhakelsifq tqlahykktv ealklelvnr sesqqtaqae lneyksklne tkvqsertrh
      421 vqnlehqkek emlteqiklh klqleemtsk yiatsavles kesiersleq altsaatlke
      481 eneslkfkld dlssrysaaq sliensqahe rtlsnkiydl ekslsrlsgi nastlselne
      541 ttyqtfdeva iqyqltkqrl eekaelekll vqrienlekd vrksreeleq anlvkksyek
      601 qlkdmkntcd kykselsslr nesgqssfql deekklssqk hnvadllrkt eedqqeikkl
      661 kaileqkete lseslkkmyd lserlkksee eteqlkhgla sawaqcaeve eklnqtlals
      721 dskldvsvps ssyssalkqf rlgrvanesv ntshdqsmdt nkilydkdeq idsesrarlq
      781 vkltsvseen erlskelecl mekqanyeei knklkhysml aenlsteker tneenktlkk
      841 klenlhslqe sinqmrveke slrkeieala cvhdeqitai kaetaseirk vqslmlglka
      901 gttelsdlki elemrhakem eelrtyfeqk cllmekqyse eifsqqskkm sdndseiadl
      961 teglyfgagg dclnvsnisg kssrlgspit ddnlkqnsns fnnqsrsele yktnvkalqq
     1021 elqnkiieaq emklnyektl eeqksvhere lydikakerh dflrsvvnqh cqtewdagal
     1081 engeltqlra aydhqleeqi alakldivna lqeqiqalla vesdsednwp pellklrdkl
     1141 tnnakremqq lkethavelq rlkeeysrnv armidrhqee lnkfrldgac dncgrielns
     1201 itdnkileer nslnktcatl kslieeliky fviceeevnn tlitevlkrq lsdslsnvkt
     1261 qkmdeldask rslesdtlns samrvqrvhf apqstgivsi insntetlpt ileededite
     1321 klkqelnncv rrlksesaei lgisfatiea qrssfskdtw mdkvneelnl klrqaealim
     1381 gyqeeaeqlk vtildlqrkl isaeskkeii tegygendei gtditledfs qlhekarhvl
     1441 snggadcsel lqlleglcrh ndkltedakr eredlqqqqv pleptpppyi hrvccrkida
     1501 adkqlkatrr fldeqasere aerdeaarqi hllqeqlker erekerdlri ssevevlesq
     1561 mremssmmsd teakktetes elkaaidkiw vlreiitele qqlqikteke eslqmqinql
     1621 etviaaqtkn qhelvqelda vkmgseskhl nehinqlqee lrkhklsseq fkvnssalkq
     1681 mktelrdmqn qldkrirdle sahmcssnls lsqpsedvsi reqidasrcp tpddptsppm
     1741 lpldqllklk ekmlkharae evafkrikdl emqvtafknq neelqaeqei lqqtaseqlf
     1801 qieamrgrle qhkqsapfaq rqatsrlelq lheanakfqs lertiadkdl elkdvrsqld
     1861 rvnqllqeke aeianvvqve natiqklreh ievleeekki lqakvgvqeh aqlelprlid
     1921 smladkneei dhlkeqlskk ekqldlyssl nldetqlrel vrqteaknsa rtlsdilsih
     1981 seceetveai rglnpaqtlp nistfkvpta avasktvdds vplmdlqvpp ldlgshsysp
     2041 tsnqqsgmid llhggsgsri stsenhfsnp ssqttqestq mqesrrqecn knessnvsvk
     2101 ctqtslnetm kevedlenql qaiqeelqik smilekrese ligmqkiyne lqaefketve
     2161 tltrdkcfyq nqyelsqvse nkikedlqev envlklrtde iqeqknkmqv nekiimelnq
     2221 entklkkdve ekeqeharky nilwqekmee lqnfraaile kditietiqt rnieiedenk
     2281 qlyefktkyq stkqemlecq neiqrltegl nnrdqiirrl eemarrtsys gtsspsnekd
     2341 qeihhlqeyl kekdkvirqm sddskslhka letiqnkmke sgnvielrkk lkderklnse
     2401 lrnmveklsk elfdlkssaq rsqddtdied mvqrelnlsa hldrqimnai esdpeekarr
     2461 venqvqyqey qegrlmdlkl klsqvnkine elkklkddle iergmlkcqi aeyegrifql
     2521 ksdlteeskk lekleeelss ernlvrtlki qiekehramq sghiqdseli eflqnklkts
     2581 ldneaklrnd lstlrqehks leiqlslmkd hvesqktdel pkladllege rrkylslmen
     2641 fekeerdnae lkdtlkklqm eksrfekqle veveekekli sglaliegik dhlqtdlrrt
     2701 keelrareee cdwlqkrikt mldaesrrqe qrtsehnelk alrreisntr evmmdleadm
     2761 kqskreltes leremklaea leklkeretd llkklsagke eerklkdtil elqhdfrtst
     2821 rtesesttef ksrytneksv pvkyiqkike lseinekymn eigmihekli etreekeqln
     2881 rkvrlleaqv krikdsdngn lmgpnytekl qhfygkylra dsrrkaltyq krylltiisg
     2941 yqlseentls vlaqltkeqr tyttmghsrk spkirfknav lvlisihrmk wlilrwssgk
     3001 rigvktllwn adqafaqvpk vamnhsppvr errttngegg fdglaleqyy qrlkniqqtl
     3061 glamaesgsr piipe
//
LOCUS       XP_034190309            3056 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608800 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190309
VERSION     XP_034190309.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334418.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190309.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3056
                     /product="uncharacterized protein LOC117608800 isoform X6"
                     /calculated_mol_wt=354194
     Region          <9..261
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          141..>870
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          828..1683
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1472..2365
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <2083..2874
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2897..2977
                     /region_name="PACT_coil_coil"
                     /note="Pericentrin-AKAP-450 domain of centrosomal
                     targeting protein; pfam10495"
                     /db_xref="CDD:463115"
     CDS             1..3056
                     /gene="LOC117608800"
                     /coded_by="XM_034334418.2:206..9376"
                     /db_xref="GeneID:117608800"
ORIGIN      
        1 mskatddder rrrsleagre mlekykvera kvqgtshsqm geasdeeslh ndkslgrres
       61 yahegissrd vtqssvsmse geadgdlegl agriaqleel lqgkeaivea lnaeidhlra
      121 etsspnssqs qnssihsrdv islyhiklqe fekavnqrdn lieeltwsvq halsardnla
      181 aqvnslnaml enanavsnkn lqekidtlen tlsdqkstiq klngqliqiq ehvqtlemek
      241 etrnaeindy klqignlneq irigaadknl niaetleqqk qyearvdkik qdmqhilkkf
      301 taetnintar hqqevkdlva kheteivniq ekyeerlkql keenkifadr lnkelpdles
      361 rhakelsifq tqlahykktv ealklelvnr sesqqtaqae lneyksklne tkvqsertrh
      421 vqnlehqkek emlteqiklh klqleemtsk yiatsavles kesiersleq altsaatlke
      481 eneslkfkld dlssrysaaq sliensqahe rtlsnkiydl ekslsrlsgi nastlselne
      541 ttyqtfdeva iqyqltkqrl eekaelekll vqrienlekd vrksreeleq anlvkksyek
      601 qlkdmkntcd kykselsslr nesgqssfql deekklssqk hnvadllrkt eedqqeikkl
      661 kaileqkete lseslkkmyd lserlkksee eteqlkhgla sawaqcaeve eklnqtlals
      721 dskldvsvps ssyssalkqf rlgrvanesv ntshdqsmdt nkilydkdeq idsesrarlq
      781 vkltsvseen erlskelecl mekqanyeei knklkhysml aenlsteker tneenktlkk
      841 klenlhslqe sinqmrveke slrkeieala cvhdeqitai kaetaseirk vqslmlglka
      901 gttelsdlki elemrhakem eelrtyfeqk cllmekqyse eifsqqskkm sdndseiadl
      961 teglyfgagg dclnvsnisg kssrlgspit ddnlkqnsns fnnqsrsele yktnvkalqq
     1021 elqnkiieaq emklnyektl eeqksvhere lydikakerh dflrsvvnqh cqtewdagal
     1081 engeltqlra aydhqleeqi alakldivna lqeqiqalla vesdsednwp pellklrdkl
     1141 tnnakremqq lkethavelq rlkeeysrnv armidrhqee lnkfrldgac dncgrielns
     1201 itdnkileer nslnktcatl kslieeliky fviceeevnn tlitevlkrq lsdslsnvkt
     1261 qkmdeldask rslesdtlns samrvqrvhf apqstgivsi insntetlpt ileededite
     1321 klkqelnncv rrlksesaei lgisfatiea qrssfskdtw mdkvneelnl klrqaealim
     1381 gyqeeaeqlk vtildlqrkl isaeskkeii tegygendei gtditledfs qlhekarhvl
     1441 snggadcsel lqlleglcrh ndkltedakr eredlqqqid aadkqlkatr rfldeqaser
     1501 eaerdeaarq ihllqeqlke rerekerdlr issevevles qmremssmms dteakktete
     1561 selkaaidki wvlreiitel eqqlqiktek eeslqmqinq letviaaqtk nqhelvqeld
     1621 avkmgseskh lnehinqlqe elrkhklsse qfkvnssalk qmktelrdmq nqldkrirdl
     1681 esahmcssnl slsqpsedvs ireqidasrc ptpddptspp mlpldqllkl kekmlkhara
     1741 eevafkrikd lemqvtafkn qneelqaeqe ilqqtaseql fqieamrgrl eqhkqsapfa
     1801 qrqatsrlel qlheanakfq slertiadkd lelkdvrsql drvnqllqek eaeianvvqv
     1861 enatiqklre hievleeekk ilqakvgvqe haqlelprli dsmladknee idhlkeqlsk
     1921 kekqldlyss lnldetqlre lvrqteakns artlsdilsi hseceetvea irglnpaqtl
     1981 pnistfkvpt aavasktvdd svplmdlqvp pldlgshsys ptsnqqsgmi dllhggsgsr
     2041 istsenhfsn pssqttqest qmqesrrqec nknessnvsv kctqtslnet mkevedlenq
     2101 lqaiqeelqi ksmilekres eligmqkiyn elqaefketv etltrdkcfy qnqyelsqvs
     2161 enkikedlqe venvlklrtd eiqeqknkmq vnekiimeln qentklkkdv eekeqehark
     2221 ynilwqekme elqnfraail ekditietiq trnieieden kqlyefktky qstkqemlec
     2281 qneiqrlteg lnnrdqiirr leemarrtsy sgtsspsnek dqeihhlqey lkekdkvirq
     2341 msddskslhk aletiqnkmk esgnvielrk klkderklns elrnmvekls kelfdlkssa
     2401 qrsqddtdie dmvqrelnls ahldrqimna iesdpeekar rvenqvqyqe yqegrlmdlk
     2461 lklsqvnkin eelkklkddl eiergmlkcq iaeyegrifq lksdlteesk klekleeels
     2521 sernlvrtlk iqiekehram qsghiqdsel ieflqnklkt sldneaklrn dlstlrqehk
     2581 sleiqlslmk dhvesqktde lpkladlleg errkylslme nfekeerdna elkdtlkklq
     2641 meksrfekql eveveekekl isglaliegi kdhlqtdlrr tkeelraree ecdwlqkrik
     2701 tmldaesrrq eqrtsehnel kalrreisnt revmmdlead mkqskrelte sleremklae
     2761 aleklkeret dllkklsagk eeerklkdti lelqhdfrts trtesestte fksrytneks
     2821 vpvkyiqkik elseinekym neigmihekl ietreekeql nrkvrlleaq vkrikdsdng
     2881 nlmgpnytek lqhfygkylr adsrrkalty qkrylltiis gyqlseentl svlaqltkeq
     2941 rtyttmghsr kspkirfkna vlvlisihrm kwlilrwssg krigvktllw nadqafaqvp
     3001 kvamnhsppv rerrttngeg gfdglaleqy yqrlkniqqt lglamaesgs rpiipe
//
LOCUS       XP_034190310            3051 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608800 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190310
VERSION     XP_034190310.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334419.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190310.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3051
                     /product="uncharacterized protein LOC117608800 isoform X7"
                     /calculated_mol_wt=353400
     Region          <9..261
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          141..>870
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          828..1678
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1549..2360
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <2078..2869
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2892..2972
                     /region_name="PACT_coil_coil"
                     /note="Pericentrin-AKAP-450 domain of centrosomal
                     targeting protein; pfam10495"
                     /db_xref="CDD:463115"
     CDS             1..3051
                     /gene="LOC117608800"
                     /coded_by="XM_034334419.2:206..9361"
                     /db_xref="GeneID:117608800"
ORIGIN      
        1 mskatddder rrrsleagre mlekykvera kvqgtshsqm geasdeeslh ndkslgrres
       61 yahegissrd vtqssvsmse geadgdlegl agriaqleel lqgkeaivea lnaeidhlra
      121 etsspnssqs qnssihsrdv islyhiklqe fekavnqrdn lieeltwsvq halsardnla
      181 aqvnslnaml enanavsnkn lqekidtlen tlsdqkstiq klngqliqiq ehvqtlemek
      241 etrnaeindy klqignlneq irigaadknl niaetleqqk qyearvdkik qdmqhilkkf
      301 taetnintar hqqevkdlva kheteivniq ekyeerlkql keenkifadr lnkelpdles
      361 rhakelsifq tqlahykktv ealklelvnr sesqqtaqae lneyksklne tkvqsertrh
      421 vqnlehqkek emlteqiklh klqleemtsk yiatsavles kesiersleq altsaatlke
      481 eneslkfkld dlssrysaaq sliensqahe rtlsnkiydl ekslsrlsgi nastlselne
      541 ttyqtfdeva iqyqltkqrl eekaelekll vqrienlekd vrksreeleq anlvkksyek
      601 qlkdmkntcd kykselsslr nesgqssfql deekklssqk hnvadllrkt eedqqeikkl
      661 kaileqkete lseslkkmyd lserlkksee eteqlkhgla sawaqcaeve eklnqtlals
      721 dskldvsvps ssyssalkqf rlgrvanesv ntshdqsmdt nkilydkdeq idsesrarlq
      781 vkltsvseen erlskelecl mekqanyeei knklkhysml aenlsteker tneenktlkk
      841 klenlhslqe sinqmrveke slrkeieala cvhdeqitai kaetaseirk vqslmlglka
      901 gttelsdlki elemrhakem eelrtyfeqk cllmekqyse eifsqqskkm sdndseiadl
      961 teglyfgagg dclnvsnisg kssrlgspit ddnlkqnsns fnnqsrsele yktnvkalqq
     1021 elqnkiieaq emklnyektl eeqksvhere lydikakerh dflrsvvnqa llavesdsed
     1081 nwppellklr dkltnnakre mqqlkethav elqrlkeeys rnvarmidrh qeelnkfrld
     1141 gacdncgrie lnsitdnkil eernslnktc atlkslieel ikyfviceee vnntlitevl
     1201 krqlsdslsn vktqkmdeld askrslesdt lnssamrvqr vhfapqstgi vsiinsntet
     1261 lptileeded iteklkqeln ncvrrlkses aeilgisfat ieaqrssfsk dtwmdkvnee
     1321 lnlklrqaea limgyqeeae qlkvtildlq rklisaeskk eiitegygen deigtditle
     1381 dfsqlhekar hvlsnggadc sellqllegl crhndklted akreredlqq qqvpleptpp
     1441 pyihrvccrk idaadkqlka trrfldeqas ereaerdeaa rqihllqeql kererekerd
     1501 lrisseqsql speaasevhv iqasaskaav evlesqmrem ssmmsdteak kteteselka
     1561 aidkiwvlre iiteleqqlq iktekeeslq mqinqletvi aaqtknqhel vqeldavkmg
     1621 seskhlnehi nqlqeelrkh klsseqfkvn ssalkqmkte lrdmqnqldk rirdlesahm
     1681 cssnlslsqp sedvsireqi dasrcptpdd ptsppmlpld qllklkekml kharaeevaf
     1741 krikdlemqv tafknqneel qaeqeilqqt aseqlfqiea mrgrleqhkq sapfaqrqat
     1801 srlelqlhea nakfqslert iadkdlelkd vrsqldrvnq llqekeaeia nvvqvenati
     1861 qklrehievl eeekkilqak vgvqehaqle lprlidsmla dkneeidhlk eqlskkekql
     1921 dlysslnlde tqlrelvrqt eaknsartls dilsihsece etveairgln paqtlpnist
     1981 fkvptaavas ktvddsvplm dlqvppldlg shsysptsnq qsgmidllhg gsgsristse
     2041 nhfsnpssqt tqestqmqes rrqecnknes snvsvkctqt slnetmkeve dlenqlqaiq
     2101 eelqiksmil ekreseligm qkiynelqae fketvetltr dkcfyqnqye lsqvsenkik
     2161 edlqevenvl klrtdeiqeq knkmqvneki imelnqentk lkkdveekeq eharkynilw
     2221 qekmeelqnf raailekdit ietiqtrnie iedenkqlye fktkyqstkq emlecqneiq
     2281 rlteglnnrd qiirrleema rrtsysgtss psnekdqeih hlqeylkekd kvirqmsdds
     2341 kslhkaleti qnkmkesgnv ielrkklkde rklnselrnm veklskelfd lkssaqrsqd
     2401 dtdiedmvqr elnlsahldr qimnaiesdp eekarrvenq vqyqeyqegr lmdlklklsq
     2461 vnkineelkk lkddleierg mlkcqiaeye grifqlksdl teeskklekl eeelssernl
     2521 vrtlkiqiek ehramqsghi qdselieflq nklktsldne aklrndlstl rqehksleiq
     2581 lslmkdhves qktdelpkla dllegerrky lslmenfeke erdnaelkdt lkklqmeksr
     2641 fekqleveve ekeklisgla liegikdhlq tdlrrtkeel rareeecdwl qkriktmlda
     2701 esrrqeqrts ehnelkalrr eisntrevmm dleadmkqsk relteslere mklaealekl
     2761 keretdllkk lsagkeeerk lkdtilelqh dfrtstrtes esttefksry tneksvpvky
     2821 iqkikelsei nekymneigm iheklietre ekeqlnrkvr lleaqvkrik dsdngnlmgp
     2881 nyteklqhfy gkylradsrr kaltyqkryl ltiisgyqls eentlsvlaq ltkeqrtytt
     2941 mghsrkspki rfknavlvli sihrmkwlil rwssgkrigv ktllwnadqa faqvpkvamn
     3001 hsppvrerrt tngeggfdgl aleqyyqrlk niqqtlglam aesgsrpiip e
//
LOCUS       XP_034190230            1056 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190230
VERSION     XP_034190230.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190230.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1056
                     /product="patched domain-containing protein isoform X1"
                     /calculated_mol_wt=119497
     Region          65..865
                     /region_name="Patched"
                     /note="Patched family; pfam02460"
                     /db_xref="CDD:308203"
     CDS             1..1056
                     /gene="Ptr"
                     /coded_by="XM_034334339.2:1385..4555"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mtlffrdrnt dhmwsnltcv ddflnrafhk lglivgrypg yfvvipvlla cicftgyqri
       61 hyeidpeylf sptngpskte raiveqyfkv nyshhfdlsr itrpgrfghv ivttkdgdsn
      121 llrkavfdel leldgliksa kavfegeeft ysqicarwld tcfsnnildl hhviedvenr
      181 elnltfpvtl npvtwdihll pvffggsvin edliiesvps lqlayflaad narqdaigat
      241 weeafldalk kvedegvfkh iatarfasrt leleleentk tivpyfsstf ilmalfsvvt
      301 cmmtdwvrsk pwlgllgnis aamatvaafg lciylgvdfi glnlaapflm igigiddtfv
      361 mlaawrrtsi lkpvpermaa tlseaavsit itsltdmisf figilspfps vqifciysgf
      421 avvftfvfhl tfftgcvais gyceqknlhs ivcckvqpls ksshrswfyr alctggvdpd
      481 dpynpvdnpe hgcmswfrdy lasalncrpv kaviififgc yivgalyglt tlregldrrk
      541 lskndsysii fydrqdyyfr efpyriqvvv sgeynyndpv vqqqmenltr aleaskyiss
      601 apiyteswlr tflsyvnnnl nevdvkdeks fikvlkdtwl spknsfsldv kfdeqeehii
      661 asrfliqavn vsgtnqekdm vkelrrictd splnasvfhp yfvffdqfel vkptsiqcmv
      721 fgalimmlis fifipnvlcc lwvafciisi elgvagymal wdvnldsism inlimcigfs
      781 vdftahicya ymsskqkspd drvkeslysl glpivqgaas tilglialll agtyifmvff
      841 kmvflvifig amhgmfllpv llslfgpssy snerieseka qqqkkmqekd lssklqqpyv
      901 iphptlsyyq psgaakslqg spatslvafe erdlglgtse dsnstesgss qsrrregqge
      961 qenqrrhdvw rrsigvlygm sqfqtaahpa apppdyagtl qelqadqdrr avrtvpyvgs
     1021 hpayrpanql hrdhrrsrsy hnlqhpsryl sdpryp
//
LOCUS       XP_076545523            1044 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545523
VERSION     XP_076545523.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689408.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="patched domain-containing protein isoform X2"
                     /calculated_mol_wt=117962
     Region          53..853
                     /region_name="Patched"
                     /note="Patched family; pfam02460"
                     /db_xref="CDD:308203"
     CDS             1..1044
                     /gene="Ptr"
                     /coded_by="XM_076689408.1:2301..5435"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mwsnltcvdd flnrafhklg livgrypgyf vvipvllaci cftgyqrihy eidpeylfsp
       61 tngpsktera iveqyfkvny shhfdlsrit rpgrfghviv ttkdgdsnll rkavfdelle
      121 ldgliksaka vfegeeftys qicarwldtc fsnnildlhh viedvenrel nltfpvtlnp
      181 vtwdihllpv ffggsvined liiesvpslq layflaadna rqdaigatwe eafldalkkv
      241 edegvfkhia tarfasrtle leleentkti vpyfsstfil malfsvvtcm mtdwvrskpw
      301 lgllgnisaa matvaafglc iylgvdfigl nlaapflmig igiddtfvml aawrrtsilk
      361 pvpermaatl seaavsitit sltdmisffi gilspfpsvq ifciysgfav vftfvfhltf
      421 ftgcvaisgy ceqknlhsiv cckvqplsks shrswfyral ctggvdpddp ynpvdnpehg
      481 cmswfrdyla salncrpvka viififgcyi vgalyglttl regldrrkls kndsysiify
      541 drqdyyfref pyriqvvvsg eynyndpvvq qqmenltral easkyissap iyteswlrtf
      601 lsyvnnnlne vdvkdeksfi kvlkdtwlsp knsfsldvkf deqeehiias rfliqavnvs
      661 gtnqekdmvk elrrictdsp lnasvfhpyf vffdqfelvk ptsiqcmvfg alimmlisfi
      721 fipnvlcclw vafciisiel gvagymalwd vnldsismin limcigfsvd ftahicyaym
      781 sskqkspddr vkeslyslgl pivqgaasti lglialllag tyifmvffkm vflvifigam
      841 hgmfllpvll slfgpssysn eriesekaqq qkkmqekdls sklqqpyvip hptlsyyqps
      901 gaakslqgsp atslvafeer dlglgtseds nstesgssqs rrregqgeqe nqrrhdvwrr
      961 sigvlygmsq fqtaahpaap ppdyagtlqe lqadqdrrav rtvpyvgshp ayrpanqlhr
     1021 dhrrsrsyhn lqhpsrylsd pryp
//
LOCUS       XP_076545524            1044 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545524
VERSION     XP_076545524.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689409.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="patched domain-containing protein isoform X2"
                     /calculated_mol_wt=117962
     Region          53..853
                     /region_name="Patched"
                     /note="Patched family; pfam02460"
                     /db_xref="CDD:308203"
     CDS             1..1044
                     /gene="Ptr"
                     /coded_by="XM_076689409.1:142..3276"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mwsnltcvdd flnrafhklg livgrypgyf vvipvllaci cftgyqrihy eidpeylfsp
       61 tngpsktera iveqyfkvny shhfdlsrit rpgrfghviv ttkdgdsnll rkavfdelle
      121 ldgliksaka vfegeeftys qicarwldtc fsnnildlhh viedvenrel nltfpvtlnp
      181 vtwdihllpv ffggsvined liiesvpslq layflaadna rqdaigatwe eafldalkkv
      241 edegvfkhia tarfasrtle leleentkti vpyfsstfil malfsvvtcm mtdwvrskpw
      301 lgllgnisaa matvaafglc iylgvdfigl nlaapflmig igiddtfvml aawrrtsilk
      361 pvpermaatl seaavsitit sltdmisffi gilspfpsvq ifciysgfav vftfvfhltf
      421 ftgcvaisgy ceqknlhsiv cckvqplsks shrswfyral ctggvdpddp ynpvdnpehg
      481 cmswfrdyla salncrpvka viififgcyi vgalyglttl regldrrkls kndsysiify
      541 drqdyyfref pyriqvvvsg eynyndpvvq qqmenltral easkyissap iyteswlrtf
      601 lsyvnnnlne vdvkdeksfi kvlkdtwlsp knsfsldvkf deqeehiias rfliqavnvs
      661 gtnqekdmvk elrrictdsp lnasvfhpyf vffdqfelvk ptsiqcmvfg alimmlisfi
      721 fipnvlcclw vafciisiel gvagymalwd vnldsismin limcigfsvd ftahicyaym
      781 sskqkspddr vkeslyslgl pivqgaasti lglialllag tyifmvffkm vflvifigam
      841 hgmfllpvll slfgpssysn eriesekaqq qkkmqekdls sklqqpyvip hptlsyyqps
      901 gaakslqgsp atslvafeer dlglgtseds nstesgssqs rrregqgeqe nqrrhdvwrr
      961 sigvlygmsq fqtaahpaap ppdyagtlqe lqadqdrrav rtvpyvgshp ayrpanqlhr
     1021 dhrrsrsyhn lqhpsrylsd pryp
//
LOCUS       XP_034190232            1044 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190232
VERSION     XP_034190232.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334341.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190232.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="patched domain-containing protein isoform X2"
                     /calculated_mol_wt=117962
     Region          53..853
                     /region_name="Patched"
                     /note="Patched family; pfam02460"
                     /db_xref="CDD:308203"
     CDS             1..1044
                     /gene="Ptr"
                     /coded_by="XM_034334341.2:329..3463"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mwsnltcvdd flnrafhklg livgrypgyf vvipvllaci cftgyqrihy eidpeylfsp
       61 tngpsktera iveqyfkvny shhfdlsrit rpgrfghviv ttkdgdsnll rkavfdelle
      121 ldgliksaka vfegeeftys qicarwldtc fsnnildlhh viedvenrel nltfpvtlnp
      181 vtwdihllpv ffggsvined liiesvpslq layflaadna rqdaigatwe eafldalkkv
      241 edegvfkhia tarfasrtle leleentkti vpyfsstfil malfsvvtcm mtdwvrskpw
      301 lgllgnisaa matvaafglc iylgvdfigl nlaapflmig igiddtfvml aawrrtsilk
      361 pvpermaatl seaavsitit sltdmisffi gilspfpsvq ifciysgfav vftfvfhltf
      421 ftgcvaisgy ceqknlhsiv cckvqplsks shrswfyral ctggvdpddp ynpvdnpehg
      481 cmswfrdyla salncrpvka viififgcyi vgalyglttl regldrrkls kndsysiify
      541 drqdyyfref pyriqvvvsg eynyndpvvq qqmenltral easkyissap iyteswlrtf
      601 lsyvnnnlne vdvkdeksfi kvlkdtwlsp knsfsldvkf deqeehiias rfliqavnvs
      661 gtnqekdmvk elrrictdsp lnasvfhpyf vffdqfelvk ptsiqcmvfg alimmlisfi
      721 fipnvlcclw vafciisiel gvagymalwd vnldsismin limcigfsvd ftahicyaym
      781 sskqkspddr vkeslyslgl pivqgaasti lglialllag tyifmvffkm vflvifigam
      841 hgmfllpvll slfgpssysn eriesekaqq qkkmqekdls sklqqpyvip hptlsyyqps
      901 gaakslqgsp atslvafeer dlglgtseds nstesgssqs rrregqgeqe nqrrhdvwrr
      961 sigvlygmsq fqtaahpaap ppdyagtlqe lqadqdrrav rtvpyvgshp ayrpanqlhr
     1021 dhrrsrsyhn lqhpsrylsd pryp
//
LOCUS       XP_034190234            1044 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190234
VERSION     XP_034190234.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334343.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190234.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="patched domain-containing protein isoform X2"
                     /calculated_mol_wt=117962
     Region          53..853
                     /region_name="Patched"
                     /note="Patched family; pfam02460"
                     /db_xref="CDD:308203"
     CDS             1..1044
                     /gene="Ptr"
                     /coded_by="XM_034334343.2:853..3987"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mwsnltcvdd flnrafhklg livgrypgyf vvipvllaci cftgyqrihy eidpeylfsp
       61 tngpsktera iveqyfkvny shhfdlsrit rpgrfghviv ttkdgdsnll rkavfdelle
      121 ldgliksaka vfegeeftys qicarwldtc fsnnildlhh viedvenrel nltfpvtlnp
      181 vtwdihllpv ffggsvined liiesvpslq layflaadna rqdaigatwe eafldalkkv
      241 edegvfkhia tarfasrtle leleentkti vpyfsstfil malfsvvtcm mtdwvrskpw
      301 lgllgnisaa matvaafglc iylgvdfigl nlaapflmig igiddtfvml aawrrtsilk
      361 pvpermaatl seaavsitit sltdmisffi gilspfpsvq ifciysgfav vftfvfhltf
      421 ftgcvaisgy ceqknlhsiv cckvqplsks shrswfyral ctggvdpddp ynpvdnpehg
      481 cmswfrdyla salncrpvka viififgcyi vgalyglttl regldrrkls kndsysiify
      541 drqdyyfref pyriqvvvsg eynyndpvvq qqmenltral easkyissap iyteswlrtf
      601 lsyvnnnlne vdvkdeksfi kvlkdtwlsp knsfsldvkf deqeehiias rfliqavnvs
      661 gtnqekdmvk elrrictdsp lnasvfhpyf vffdqfelvk ptsiqcmvfg alimmlisfi
      721 fipnvlcclw vafciisiel gvagymalwd vnldsismin limcigfsvd ftahicyaym
      781 sskqkspddr vkeslyslgl pivqgaasti lglialllag tyifmvffkm vflvifigam
      841 hgmfllpvll slfgpssysn eriesekaqq qkkmqekdls sklqqpyvip hptlsyyqps
      901 gaakslqgsp atslvafeer dlglgtseds nstesgssqs rrregqgeqe nqrrhdvwrr
      961 sigvlygmsq fqtaahpaap ppdyagtlqe lqadqdrrav rtvpyvgshp ayrpanqlhr
     1021 dhrrsrsyhn lqhpsrylsd pryp
//
LOCUS       XP_034190235            1044 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190235
VERSION     XP_034190235.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334344.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190235.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="patched domain-containing protein isoform X2"
                     /calculated_mol_wt=117962
     Region          53..853
                     /region_name="Patched"
                     /note="Patched family; pfam02460"
                     /db_xref="CDD:308203"
     CDS             1..1044
                     /gene="Ptr"
                     /coded_by="XM_034334344.2:724..3858"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mwsnltcvdd flnrafhklg livgrypgyf vvipvllaci cftgyqrihy eidpeylfsp
       61 tngpsktera iveqyfkvny shhfdlsrit rpgrfghviv ttkdgdsnll rkavfdelle
      121 ldgliksaka vfegeeftys qicarwldtc fsnnildlhh viedvenrel nltfpvtlnp
      181 vtwdihllpv ffggsvined liiesvpslq layflaadna rqdaigatwe eafldalkkv
      241 edegvfkhia tarfasrtle leleentkti vpyfsstfil malfsvvtcm mtdwvrskpw
      301 lgllgnisaa matvaafglc iylgvdfigl nlaapflmig igiddtfvml aawrrtsilk
      361 pvpermaatl seaavsitit sltdmisffi gilspfpsvq ifciysgfav vftfvfhltf
      421 ftgcvaisgy ceqknlhsiv cckvqplsks shrswfyral ctggvdpddp ynpvdnpehg
      481 cmswfrdyla salncrpvka viififgcyi vgalyglttl regldrrkls kndsysiify
      541 drqdyyfref pyriqvvvsg eynyndpvvq qqmenltral easkyissap iyteswlrtf
      601 lsyvnnnlne vdvkdeksfi kvlkdtwlsp knsfsldvkf deqeehiias rfliqavnvs
      661 gtnqekdmvk elrrictdsp lnasvfhpyf vffdqfelvk ptsiqcmvfg alimmlisfi
      721 fipnvlcclw vafciisiel gvagymalwd vnldsismin limcigfsvd ftahicyaym
      781 sskqkspddr vkeslyslgl pivqgaasti lglialllag tyifmvffkm vflvifigam
      841 hgmfllpvll slfgpssysn eriesekaqq qkkmqekdls sklqqpyvip hptlsyyqps
      901 gaakslqgsp atslvafeer dlglgtseds nstesgssqs rrregqgeqe nqrrhdvwrr
      961 sigvlygmsq fqtaahpaap ppdyagtlqe lqadqdrrav rtvpyvgshp ayrpanqlhr
     1021 dhrrsrsyhn lqhpsrylsd pryp
//
LOCUS       XP_034190237             165 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190237
VERSION     XP_034190237.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334346.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190237.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..165
                     /product="patched domain-containing protein isoform X3"
                     /calculated_mol_wt=18916
     Region          <2..>143
                     /region_name="2A060601"
                     /note="Niemann-Pick C type protein family; TIGR00917"
                     /db_xref="CDD:273337"
     CDS             1..165
                     /gene="Ptr"
                     /coded_by="XM_034334346.2:1..498"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mwsnltcvdd flnrafhklg livgrypgyf vvipvllaci cftgyqrihy eidpeylfsp
       61 tngpsktera iveqyfkvny shhfdlsrit rpgrfghviv ttkdgdsnll rkavfdelle
      121 ldgliksaka vfegeeftys qicarwldtc fsnnildlhh vidtf
//
LOCUS       XP_034190238             164 aa            linear   INV 03-SEP-2025
DEFINITION  patched domain-containing protein isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190238
VERSION     XP_034190238.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334347.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190238.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..164
                     /product="patched domain-containing protein isoform X4"
                     /calculated_mol_wt=18824
     Region          <2..>143
                     /region_name="2A060601"
                     /note="Niemann-Pick C type protein family; TIGR00917"
                     /db_xref="CDD:273337"
     CDS             1..164
                     /gene="Ptr"
                     /coded_by="XM_034334347.2:1..495"
                     /db_xref="GeneID:117608764"
ORIGIN      
        1 mwsnltcvdd flnrafhklg livgrypgyf vvipvllaci cftgyqrihy eidpeylfsp
       61 tngpsktera iveqyfkvny shhfdlsrit rpgrfghviv ttkdgdsnll rkavfdelle
      121 ldgliksaka vfegeeftys qicarwldtc fsnnildlhh vidr
//
LOCUS       XP_034190311             820 aa            linear   INV 03-SEP-2025
DEFINITION  tsr1 ribosome assembly factor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190311
VERSION     XP_034190311.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334420.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190311.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="tsr1 ribosome assembly factor isoform X1"
                     /calculated_mol_wt=93409
     Region          1..>65
                     /region_name="Csa3"
                     /note="CRISPR-associated protein Csa3, CARF domain
                     [Defense mechanisms]; COG3415"
                     /db_xref="CDD:442641"
     Region          68..798
                     /region_name="COG5177"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227504"
     Region          511..798
                     /region_name="RIBIOP_C"
                     /note="40S ribosome biogenesis protein Tsr1 and BMS1
                     C-terminal; pfam04950"
                     /db_xref="CDD:461497"
     CDS             1..820
                     /gene="Tsr1"
                     /coded_by="XM_034334420.2:61..2523"
                     /db_xref="GeneID:117608801"
ORIGIN      
        1 mlsakderll qkgaiialhk agnsnveiar iaqcsvrtvq ryvvrysvyg teglvdlrql
       61 nrgkpgvkvv skrirknlgk darrhqssqi rqkkreevlq qkrdlggsqs apilvciipl
      121 qedldtqsil svltkadeta vvtnspcgit hlsiprfkqr fsvivppvgn vfatldaaki
      181 sttilfvasa inqddksprn evldnwgrei ilpcvaqglt tpavaltnie slhikkrqdf
      241 knnmqseisk wlpeekicpl dtstdalnvl rkvgsqkqrt vsyrskrahl laeeikfkcn
      301 edstdlgtlt vsgylrnvpl svnsiihipg fgdfqmsqid apedpypiek karkhcnkmd
      361 eepsvrvler adpkkqesle senvpdpmda eqtwpteeel aqagaaqkkk ivkrvpkgts
      421 eyqaawipde dgeelseysd eesedkmsvd iksepdsdag teedeeeydt itiseapdeq
      481 rydqnidmve ekeaieklke akldaqfpde vdtpqdilak qrfqkyrgle sfrtspwdpk
      541 enlpsdyari fqfenfdrtr krifkeceei egampgwyit ihiknvrrtl ftafftlenr
      601 plvvfgllpn ehkmsvlnvv lkhtntisip ikskerlifq cgfrrfasrp ifsqhtngnk
      661 hkyeryfqpe stivasmfap itfppcpvlc yveklngsla lvatgsvlsa npdrivvkrv
      721 vlsghpykvh krsavirfmf fhredinwfk pvelrtkygr rghikeplgt hghmkcvfng
      781 qlksqdtilm nlykrvfpkw tyepllltes sheeqsmnve
//
LOCUS       XP_034190312             820 aa            linear   INV 03-SEP-2025
DEFINITION  tsr1 ribosome assembly factor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190312
VERSION     XP_034190312.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334421.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190312.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="tsr1 ribosome assembly factor isoform X1"
                     /calculated_mol_wt=93409
     Region          1..>65
                     /region_name="Csa3"
                     /note="CRISPR-associated protein Csa3, CARF domain
                     [Defense mechanisms]; COG3415"
                     /db_xref="CDD:442641"
     Region          68..798
                     /region_name="COG5177"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227504"
     Region          511..798
                     /region_name="RIBIOP_C"
                     /note="40S ribosome biogenesis protein Tsr1 and BMS1
                     C-terminal; pfam04950"
                     /db_xref="CDD:461497"
     CDS             1..820
                     /gene="Tsr1"
                     /coded_by="XM_034334421.2:61..2523"
                     /db_xref="GeneID:117608801"
ORIGIN      
        1 mlsakderll qkgaiialhk agnsnveiar iaqcsvrtvq ryvvrysvyg teglvdlrql
       61 nrgkpgvkvv skrirknlgk darrhqssqi rqkkreevlq qkrdlggsqs apilvciipl
      121 qedldtqsil svltkadeta vvtnspcgit hlsiprfkqr fsvivppvgn vfatldaaki
      181 sttilfvasa inqddksprn evldnwgrei ilpcvaqglt tpavaltnie slhikkrqdf
      241 knnmqseisk wlpeekicpl dtstdalnvl rkvgsqkqrt vsyrskrahl laeeikfkcn
      301 edstdlgtlt vsgylrnvpl svnsiihipg fgdfqmsqid apedpypiek karkhcnkmd
      361 eepsvrvler adpkkqesle senvpdpmda eqtwpteeel aqagaaqkkk ivkrvpkgts
      421 eyqaawipde dgeelseysd eesedkmsvd iksepdsdag teedeeeydt itiseapdeq
      481 rydqnidmve ekeaieklke akldaqfpde vdtpqdilak qrfqkyrgle sfrtspwdpk
      541 enlpsdyari fqfenfdrtr krifkeceei egampgwyit ihiknvrrtl ftafftlenr
      601 plvvfgllpn ehkmsvlnvv lkhtntisip ikskerlifq cgfrrfasrp ifsqhtngnk
      661 hkyeryfqpe stivasmfap itfppcpvlc yveklngsla lvatgsvlsa npdrivvkrv
      721 vlsghpykvh krsavirfmf fhredinwfk pvelrtkygr rghikeplgt hghmkcvfng
      781 qlksqdtilm nlykrvfpkw tyepllltes sheeqsmnve
//
LOCUS       XP_034190313             795 aa            linear   INV 03-SEP-2025
DEFINITION  tsr1 ribosome assembly factor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190313
VERSION     XP_034190313.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334422.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190313.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..795
                     /product="tsr1 ribosome assembly factor isoform X2"
                     /calculated_mol_wt=90536
     Region          15..773
                     /region_name="COG5177"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227504"
     Region          486..773
                     /region_name="RIBIOP_C"
                     /note="40S ribosome biogenesis protein Tsr1 and BMS1
                     C-terminal; pfam04950"
                     /db_xref="CDD:461497"
     CDS             1..795
                     /gene="Tsr1"
                     /coded_by="XM_034334422.2:103..2490"
                     /db_xref="GeneID:117608801"
ORIGIN      
        1 mgitkqevhr pgslkqtnka hktgrhrskg sissevkgkp gvkvvskrir knlgkdarrh
       61 qssqirqkkr eevlqqkrdl ggsqsapilv ciiplqedld tqsilsvltk adetavvtns
      121 pcgithlsip rfkqrfsviv ppvgnvfatl daakisttil fvasainqdd ksprnevldn
      181 wgreiilpcv aqglttpava ltnieslhik krqdfknnmq seiskwlpee kicpldtstd
      241 alnvlrkvgs qkqrtvsyrs krahllaeei kfkcnedstd lgtltvsgyl rnvplsvnsi
      301 ihipgfgdfq msqidapedp ypiekkarkh cnkmdeepsv rvleradpkk qeslesenvp
      361 dpmdaeqtwp teeelaqaga aqkkkivkrv pkgtseyqaa wipdedgeel seysdeesed
      421 kmsvdiksep dsdagteede eeydtitise apdeqrydqn idmveekeai eklkeaklda
      481 qfpdevdtpq dilakqrfqk yrglesfrts pwdpkenlps dyarifqfen fdrtrkrifk
      541 eceeiegamp gwyitihikn vrrtlftaff tlenrplvvf gllpnehkms vlnvvlkhtn
      601 tisipikske rlifqcgfrr fasrpifsqh tngnkhkyer yfqpestiva smfapitfpp
      661 cpvlcyvekl ngslalvatg svlsanpdri vvkrvvlsgh pykvhkrsav irfmffhred
      721 inwfkpvelr tkygrrghik eplgthghmk cvfngqlksq dtilmnlykr vfpkwtyepl
      781 lltessheeq smnve
//
LOCUS       XP_034190314             602 aa            linear   INV 03-SEP-2025
DEFINITION  28S rRNA (cytosine(4447)-C(5))-methyltransferase [Osmia lignaria
            lignaria].
ACCESSION   XP_034190314
VERSION     XP_034190314.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334423.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190314.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="28S rRNA
                     (cytosine(4447)-C(5))-methyltransferase"
                     /calculated_mol_wt=68602
     Region          321..584
                     /region_name="nop2p"
                     /note="NOL1/NOP2/sun family putative RNA methylase;
                     TIGR00446"
                     /db_xref="CDD:188051"
     CDS             1..602
                     /gene="LOC117608802"
                     /coded_by="XM_034334423.2:152..1960"
                     /db_xref="GeneID:117608802"
ORIGIN      
        1 mgrkakfdet vvpkgpgrka kkqgdptfpk gvlvkeekil shrqkqrark rlskkqnlke
       61 lqkkqknakd ivkknnlkds skqlkkkkkp pnvvsfandn esdeneqmql dndsnvsdee
      121 tmenkkksks tkmklmdsdv edesdaades daaednkeyn ssdesdenee mdqdeeeaee
      181 ddddllpiek ankklkkkqq keqklaeeem tdmmsqqfif nfpteeelen vtslkdvqqr
      241 ikdvvmilsd fkrlrdvnrs rseymdllrk dlctyysynn flmeklmqif pldellefle
      301 asevqrpmtl rtntlrtrrr dlaealinrg vnldpigkwt kiglvvyssq vpmgatpeyl
      361 aghyilqgas sflpvmalep kenerildmc aapggksshi aalmkntgvl fsndvnveri
      421 kavvgnfhrl givnsvvsth dgrklpsiik gfdrvlldap ctgtgvvskd psvktnkdev
      481 diqrcctlqr elllaaidca narsesggii vystcsilpe enewvidyal rkrdvklipt
      541 glefgtegft syrqyrfhps lkltkrfyph vhnmdgffva klkkfsniiq kkkvteeesa
      601 vd
//
LOCUS       XP_034190315             602 aa            linear   INV 03-SEP-2025
DEFINITION  28S rRNA (cytosine(4447)-C(5))-methyltransferase [Osmia lignaria
            lignaria].
ACCESSION   XP_034190315
VERSION     XP_034190315.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334424.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190315.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="28S rRNA
                     (cytosine(4447)-C(5))-methyltransferase"
                     /calculated_mol_wt=68602
     Region          321..584
                     /region_name="nop2p"
                     /note="NOL1/NOP2/sun family putative RNA methylase;
                     TIGR00446"
                     /db_xref="CDD:188051"
     CDS             1..602
                     /gene="LOC117608802"
                     /coded_by="XM_034334424.2:126..1934"
                     /db_xref="GeneID:117608802"
ORIGIN      
        1 mgrkakfdet vvpkgpgrka kkqgdptfpk gvlvkeekil shrqkqrark rlskkqnlke
       61 lqkkqknakd ivkknnlkds skqlkkkkkp pnvvsfandn esdeneqmql dndsnvsdee
      121 tmenkkksks tkmklmdsdv edesdaades daaednkeyn ssdesdenee mdqdeeeaee
      181 ddddllpiek ankklkkkqq keqklaeeem tdmmsqqfif nfpteeelen vtslkdvqqr
      241 ikdvvmilsd fkrlrdvnrs rseymdllrk dlctyysynn flmeklmqif pldellefle
      301 asevqrpmtl rtntlrtrrr dlaealinrg vnldpigkwt kiglvvyssq vpmgatpeyl
      361 aghyilqgas sflpvmalep kenerildmc aapggksshi aalmkntgvl fsndvnveri
      421 kavvgnfhrl givnsvvsth dgrklpsiik gfdrvlldap ctgtgvvskd psvktnkdev
      481 diqrcctlqr elllaaidca narsesggii vystcsilpe enewvidyal rkrdvklipt
      541 glefgtegft syrqyrfhps lkltkrfyph vhnmdgffva klkkfsniiq kkkvteeesa
      601 vd
//
LOCUS       XP_034190295             522 aa            linear   INV 03-SEP-2025
DEFINITION  2-phosphoxylose phosphatase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190295
VERSION     XP_034190295.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334404.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="2-phosphoxylose phosphatase 1 isoform X1"
                     /calculated_mol_wt=59281
     Region          173..453
                     /region_name="HP"
                     /note="Histidine phosphatase domain found in a
                     functionally diverse set of proteins, mostly phosphatases;
                     contains a His residue which is phosphorylated during the
                     reaction; cl11399"
                     /db_xref="CDD:472174"
     CDS             1..522
                     /gene="LOC117608793"
                     /coded_by="XM_034334404.2:43..1611"
                     /db_xref="GeneID:117608793"
ORIGIN      
        1 mlaemvrlsy qhrafycyvi lsvwifllva gmykyigide kssvqtrvlg ndisirefpr
       61 nlkadiktkk ifrfcnppnd iaaetegkld gnltlggilv firhgdrgpl ahirnisivn
      121 cggelngipe lenlyqnyvn flqnvssysr tawaqflgpf hgfpmlpans kdcrigqltt
      181 lgigqllktg iilknayyhk lnlgngtiss kdivvystry rrtvqsaval lyalletdgf
      241 qnlakvtmqe sqsyafcntd cacpvaekff kqhlkemsdh lkshpavadl irqassavfe
      301 ipdqaqmtdp htlkdallty ichgaslpcm dfetqrvcvk tehvtglfay teweskqiak
      361 ssnrrkygll raygllrsiv symlriisea kpkivlysgh dktleylata lgifsdkltm
      421 phyasrfvie vyrinpknen hvasdfyfrv iingkdftqk ipfcrnanfy tvsyfernda
      481 ektrrdsklc pietiirqlh ddyfgpfnat nfkdactnhk rg
//
LOCUS       XP_034190296             493 aa            linear   INV 03-SEP-2025
DEFINITION  2-phosphoxylose phosphatase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190296
VERSION     XP_034190296.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334405.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="2-phosphoxylose phosphatase 1 isoform X2"
                     /calculated_mol_wt=55840
     Region          144..424
                     /region_name="HP"
                     /note="Histidine phosphatase domain found in a
                     functionally diverse set of proteins, mostly phosphatases;
                     contains a His residue which is phosphorylated during the
                     reaction; cl11399"
                     /db_xref="CDD:472174"
     CDS             1..493
                     /gene="LOC117608793"
                     /coded_by="XM_034334405.2:404..1885"
                     /db_xref="GeneID:117608793"
ORIGIN      
        1 mcmykyigid ekssvqtrvl gndisirefp rnlkadiktk kifrfcnppn diaaetegkl
       61 dgnltlggil vfirhgdrgp lahirnisiv ncggelngip elenlyqnyv nflqnvssys
      121 rtawaqflgp fhgfpmlpan skdcrigqlt tlgigqllkt giilknayyh klnlgngtis
      181 skdivvystr yrrtvqsava llyalletdg fqnlakvtmq esqsyafcnt dcacpvaekf
      241 fkqhlkemsd hlkshpavad lirqassavf eipdqaqmtd phtlkdallt yichgaslpc
      301 mdfetqrvcv ktehvtglfa yteweskqia kssnrrkygl lraygllrsi vsymlriise
      361 akpkivlysg hdktleylat algifsdklt mphyasrfvi evyrinpkne nhvasdfyfr
      421 viingkdftq kipfcrnanf ytvsyfernd aektrrdskl cpietiirql hddyfgpfna
      481 tnfkdactnh krg
//
LOCUS       XP_034190317             496 aa            linear   INV 03-SEP-2025
DEFINITION  pachytene checkpoint 2 protein [Osmia lignaria lignaria].
ACCESSION   XP_034190317
VERSION     XP_034190317.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334426.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190317.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="pachytene checkpoint 2 protein"
                     /calculated_mol_wt=56882
     Region          182..378
                     /region_name="RecA-like_Pch2-like"
                     /note="ATPase domain of Pachytene checkpoint 2 (Pch2) and
                     similar ATPase domains; cd19508"
                     /db_xref="CDD:410916"
     Site            order(194,211..212,215..216,222..225,227..231,275,
                     277..278,284,370)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:410916"
     Site            order(240,244..246,271,273..274,312..313)
                     /site_type="active"
                     /note="ATP binding site [active]"
                     /db_xref="CDD:410916"
     CDS             1..496
                     /gene="pch2"
                     /coded_by="XM_034334426.2:16..1506"
                     /db_xref="GeneID:117608803"
ORIGIN      
        1 mktrhkyras peprqtrtpi rglfrnspra heilgpalhk yppqqfgntc visfklylkf
       61 ffrdyqsren snkmdgpnvl hveicqnins tlsseeiekl veaeiysyed sipldiildg
      121 kklsnhilnd hvdsiicsys ygkrgqmkvp vyadvkfhvy rlttesaate tmendgedlp
      181 vsshwilpvk efhylwesly fdsnikddll hfietamifa dhnvdtniis wnkvvllhgp
      241 pgtgktslck alaqkiiirt gkrfshgefv einshslfsk wfsesgklvm klfneikrll
      301 enpnalvcil ideieslaha rkscsgtept dairvvnall tqldqikrys nvlilttsnl
      361 seaidlafid radikqyigp pkeqaiyqiy tsclkelmra klmeheeihd lshlkklgyd
      421 etlttknslk lkelseesvg lsgrilrkip flahafylrt kkctlarflr amhlavrrek
      481 egnveevsqa lhqley
//
LOCUS       XP_034190318             463 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar fusion protein CCZ1 [Osmia lignaria lignaria].
ACCESSION   XP_034190318
VERSION     XP_034190318.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334427.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..463
                     /product="vacuolar fusion protein CCZ1"
                     /calculated_mol_wt=53206
     Region          9..133
                     /region_name="Intu_longin_1"
                     /note="First Longin domain of INTU, CCZ1 and HPS4;
                     pfam19031"
                     /db_xref="CDD:436911"
     Region          206..333
                     /region_name="Intu_longin_2"
                     /note="Intu longin-like domain 2; pfam19032"
                     /db_xref="CDD:465951"
     Region          356..452
                     /region_name="Intu_longin_3"
                     /note="Intu longin-like domain 3; pfam19033"
                     /db_xref="CDD:436913"
     CDS             1..463
                     /gene="Ccz1"
                     /coded_by="XM_034334427.2:140..1531"
                     /db_xref="GeneID:117608804"
ORIGIN      
        1 mssktevtle hfyifngtya kkegeeekki lyyyperdld vqikniglse aiikfaqsfn
       61 pgqpcdycht hktrqiyyqp epnfwmvmiv gvpyickekd gnkyteyqnd evsssicqsi
      121 lkqayvmfrl fmgsfdtiin epecggitll khtlehfysr yllslklnns dildifqglq
      181 flpldkitfl kvqcfmnlve amfsqvkyta flyndqvvws glepedmqvv ynylvstllp
      241 ahlekelhgg smprnspspf ttshygkfvt gpsninepnl igksptvfin ystkpvslyl
      301 vvyralsati clfvdsktsl midffksldn flgpqlttlv ssvaeqcakh vtvtpesctk
      361 ylyfnklnla ykstihldnr rcnnvlttpe vlrvindiys dtnklkeage iviktmsdyw
      421 vigklsnlre ffvviqqksa siieiedevk rlcekqlksi ffh
//
LOCUS       XP_034190319             434 aa            linear   INV 03-SEP-2025
DEFINITION  dolichyl-diphosphooligosaccharide--protein glycosyltransferase
            non-catalytic subunit Ost48 [Osmia lignaria lignaria].
ACCESSION   XP_034190319
VERSION     XP_034190319.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334428.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..434
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase non-catalytic subunit Ost48"
                     /calculated_mol_wt=48282
     Region          24..426
                     /region_name="DDOST_48kD"
                     /note="Oligosaccharyltransferase 48 kDa subunit beta;
                     pfam03345"
                     /db_xref="CDD:460890"
     CDS             1..434
                     /gene="Ost48"
                     /coded_by="XM_034334428.2:85..1389"
                     /db_xref="GeneID:117608805"
ORIGIN      
        1 mkimnkllyf laifeivyag gpslvlldnl aikethsiff ktlqdsgytl tykladdanl
       61 qlskygeyly khliifapsv eefggalsve aitdfidggg nvlvagssqs gdalhelase
      121 cgfeideegs avidhlnydv sdngyhtkiv adpanlidap vivgskniqp llyqgtglia
      181 dvenplilrl ltasssaysy npqnpikeyp havgkntlli aalqarnnar vvfsgslyff
      241 sdeaftspiq kaqdgkkyek sgneavatmi arwvfkengv irvasvhhhr vgeseppaay
      301 timddvvysi evqklsgdqw vpyetndlql efvridpfir mtmkpvgngr yearfkipdv
      361 ygvyqfkvdy triglthlys ttqvsvrplq htqyerfips afpyyisafs mmggvflfsl
      421 vflhykedtk akse
//
LOCUS       XP_034190321             425 aa            linear   INV 03-SEP-2025
DEFINITION  C1GALT1-specific chaperone 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190321
VERSION     XP_034190321.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334430.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190321.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="C1GALT1-specific chaperone 1"
                     /calculated_mol_wt=49625
     Region          142..>254
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..425
                     /gene="LOC117608806"
                     /coded_by="XM_034334430.2:470..1747"
                     /db_xref="GeneID:117608806"
ORIGIN      
        1 mhlyrfksrs vflvgfgigi lsalllltvq hvfnnmsiye rsfmnsytan kndlskwfts
       61 vtptsqeitf qlweekkvkd rfanvtyrkw lenqqvkikd idmdtylygi kgkerleadw
      121 lksnvhltci vfvkkiklar svkdtwgkhc nkiyffgqqk dsevpvrnfd rklvsswqll
      181 ceafnyvwkd natlewlvfv kddtlvipen lrymlgplny tddyylghav vmwgqsynaa
      241 dagyviskgv lkklttmfdn sekcitggky wkqedyylak hlatmgihps dtrdqylrgt
      301 fhgyslqsll wgvakigiyw tralypahde ccsfmsvtfn vaesdkmytl nyllyhlhvf
      361 kneaifgtrn apvsvpdenv wkvalkeefn itylnnissd ayyeiwhsky sepgqliaqn
      421 yhgrn
//
LOCUS       XP_034190322             425 aa            linear   INV 03-SEP-2025
DEFINITION  C1GALT1-specific chaperone 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190322
VERSION     XP_034190322.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334431.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190322.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="C1GALT1-specific chaperone 1"
                     /calculated_mol_wt=49625
     Region          142..>254
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..425
                     /gene="LOC117608806"
                     /coded_by="XM_034334431.2:416..1693"
                     /db_xref="GeneID:117608806"
ORIGIN      
        1 mhlyrfksrs vflvgfgigi lsalllltvq hvfnnmsiye rsfmnsytan kndlskwfts
       61 vtptsqeitf qlweekkvkd rfanvtyrkw lenqqvkikd idmdtylygi kgkerleadw
      121 lksnvhltci vfvkkiklar svkdtwgkhc nkiyffgqqk dsevpvrnfd rklvsswqll
      181 ceafnyvwkd natlewlvfv kddtlvipen lrymlgplny tddyylghav vmwgqsynaa
      241 dagyviskgv lkklttmfdn sekcitggky wkqedyylak hlatmgihps dtrdqylrgt
      301 fhgyslqsll wgvakigiyw tralypahde ccsfmsvtfn vaesdkmytl nyllyhlhvf
      361 kneaifgtrn apvsvpdenv wkvalkeefn itylnnissd ayyeiwhsky sepgqliaqn
      421 yhgrn
//
LOCUS       XP_034190323             425 aa            linear   INV 03-SEP-2025
DEFINITION  C1GALT1-specific chaperone 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190323
VERSION     XP_034190323.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334432.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190323.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="C1GALT1-specific chaperone 1"
                     /calculated_mol_wt=49625
     Region          142..>254
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..425
                     /gene="LOC117608806"
                     /coded_by="XM_034334432.2:447..1724"
                     /db_xref="GeneID:117608806"
ORIGIN      
        1 mhlyrfksrs vflvgfgigi lsalllltvq hvfnnmsiye rsfmnsytan kndlskwfts
       61 vtptsqeitf qlweekkvkd rfanvtyrkw lenqqvkikd idmdtylygi kgkerleadw
      121 lksnvhltci vfvkkiklar svkdtwgkhc nkiyffgqqk dsevpvrnfd rklvsswqll
      181 ceafnyvwkd natlewlvfv kddtlvipen lrymlgplny tddyylghav vmwgqsynaa
      241 dagyviskgv lkklttmfdn sekcitggky wkqedyylak hlatmgihps dtrdqylrgt
      301 fhgyslqsll wgvakigiyw tralypahde ccsfmsvtfn vaesdkmytl nyllyhlhvf
      361 kneaifgtrn apvsvpdenv wkvalkeefn itylnnissd ayyeiwhsky sepgqliaqn
      421 yhgrn
//
LOCUS       XP_076545525             425 aa            linear   INV 03-SEP-2025
DEFINITION  C1GALT1-specific chaperone 1 [Osmia lignaria lignaria].
ACCESSION   XP_076545525
VERSION     XP_076545525.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689410.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="C1GALT1-specific chaperone 1"
                     /calculated_mol_wt=49625
     Region          142..>254
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..425
                     /gene="LOC117608806"
                     /coded_by="XM_076689410.1:302..1579"
                     /db_xref="GeneID:117608806"
ORIGIN      
        1 mhlyrfksrs vflvgfgigi lsalllltvq hvfnnmsiye rsfmnsytan kndlskwfts
       61 vtptsqeitf qlweekkvkd rfanvtyrkw lenqqvkikd idmdtylygi kgkerleadw
      121 lksnvhltci vfvkkiklar svkdtwgkhc nkiyffgqqk dsevpvrnfd rklvsswqll
      181 ceafnyvwkd natlewlvfv kddtlvipen lrymlgplny tddyylghav vmwgqsynaa
      241 dagyviskgv lkklttmfdn sekcitggky wkqedyylak hlatmgihps dtrdqylrgt
      301 fhgyslqsll wgvakigiyw tralypahde ccsfmsvtfn vaesdkmytl nyllyhlhvf
      361 kneaifgtrn apvsvpdenv wkvalkeefn itylnnissd ayyeiwhsky sepgqliaqn
      421 yhgrn
//
LOCUS       XP_034190324             425 aa            linear   INV 03-SEP-2025
DEFINITION  C1GALT1-specific chaperone 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190324
VERSION     XP_034190324.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334433.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190324.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="C1GALT1-specific chaperone 1"
                     /calculated_mol_wt=49625
     Region          142..>254
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..425
                     /gene="LOC117608806"
                     /coded_by="XM_034334433.2:257..1534"
                     /db_xref="GeneID:117608806"
ORIGIN      
        1 mhlyrfksrs vflvgfgigi lsalllltvq hvfnnmsiye rsfmnsytan kndlskwfts
       61 vtptsqeitf qlweekkvkd rfanvtyrkw lenqqvkikd idmdtylygi kgkerleadw
      121 lksnvhltci vfvkkiklar svkdtwgkhc nkiyffgqqk dsevpvrnfd rklvsswqll
      181 ceafnyvwkd natlewlvfv kddtlvipen lrymlgplny tddyylghav vmwgqsynaa
      241 dagyviskgv lkklttmfdn sekcitggky wkqedyylak hlatmgihps dtrdqylrgt
      301 fhgyslqsll wgvakigiyw tralypahde ccsfmsvtfn vaesdkmytl nyllyhlhvf
      361 kneaifgtrn apvsvpdenv wkvalkeefn itylnnissd ayyeiwhsky sepgqliaqn
      421 yhgrn
//
LOCUS       XP_034190325             365 aa            linear   INV 03-SEP-2025
DEFINITION  replication in mitochondria 2 [Osmia lignaria lignaria].
ACCESSION   XP_034190325
VERSION     XP_034190325.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="replication in mitochondria 2"
                     /calculated_mol_wt=40359
     Region          <22..146
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          152..239
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          253..340
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..365
                     /gene="Rim2"
                     /coded_by="XM_034334434.2:250..1347"
                     /db_xref="GeneID:117608807"
ORIGIN      
        1 mfdrdtaihl vaggvagtag aivtcplevv ktrlqssssg fypppvnkel tsghvtcksf
       61 pkpeqrrrlc tggytrhalv alshfgsspp egtphshspg iyqciryivq negtkalfkg
      121 lgpnlvgvap sraiyfcays kskiafnail tpdtplvhvf safcagfvac tltnpiwfvk
      181 trlqldhrsd kitamecvqr iyrqsgilgf ykgivasyvg isetvihfvi yeavkaslva
      241 yrmpddrktl rdflefmaag sfsktiassi ayphevvrtr lreegtkyyg ffqtvrtvla
      301 eegrhglyrg lgthlirqip ntaiimatye avvyilnrhf heqalinnak esqyytesks
      361 ksela
//
LOCUS       XP_034190327             312 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p63-regulated gene 1-like protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190327
VERSION     XP_034190327.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334436.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="tumor protein p63-regulated gene 1-like protein
                     isoform X1"
                     /calculated_mol_wt=35044
     Region          76..171
                     /region_name="hSac2"
                     /note="Inositol phosphatase; pfam12456"
                     /db_xref="CDD:463592"
     CDS             1..312
                     /gene="LOC117608808"
                     /coded_by="XM_034334436.2:235..1173"
                     /db_xref="GeneID:117608808"
ORIGIN      
        1 mdetyfdegp agnfetatle itkdldgnef snstsastgi dvekrnrene gnisnqvpts
       61 kdvstipfkh ldihsfftdr nevvaraikd cqdslineee kdiagswllt eislwdieke
      121 rlvvltkkai ysikydfisl kvleynripl sqldtlvsge lvypssslap rlnglaegvs
      181 sivncavrqe wssvasctgl aqfeprkrnm agvrlmwnkg nplplikkwn pfakdipwlt
      241 ytshplfwyk gseeeklkfd veglrstikd llpsecnirs gliiienyig vgalvhnrna
      301 lgffkirgkv sf
//
LOCUS       XP_034190328             276 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p63-regulated gene 1-like protein isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190328
VERSION     XP_034190328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="tumor protein p63-regulated gene 1-like protein
                     isoform X2"
                     /calculated_mol_wt=31226
     Region          76..182
                     /region_name="hSac2"
                     /note="Inositol phosphatase; pfam12456"
                     /db_xref="CDD:463592"
     CDS             1..276
                     /gene="LOC117608808"
                     /coded_by="XM_034334437.2:235..1065"
                     /db_xref="GeneID:117608808"
ORIGIN      
        1 mdetyfdegp agnfetatle itkdldgnef snstsastgi dvekrnrene gnisnqvpts
       61 kdvstipfkh ldihsfftdr nevvaraikd cqdslineee kdiagswllt eislwdieke
      121 rlvvltkkai ysikydfisl kvleynripl sqldtlvsge lvypssslap krnmagvrlm
      181 wnkgnplpli kkwnpfakdi pwltytshpl fwykgseeek lkfdveglrs tikdllpsec
      241 nirsgliiie nyigvgalvh nrnalgffki rgkvsf
//
LOCUS       XP_034190329             289 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin N-terminal domain-containing protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190329
VERSION     XP_034190329.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334438.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="cyclin N-terminal domain-containing protein 1"
                     /calculated_mol_wt=33281
     Region          47..164
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..289
                     /gene="LOC117608809"
                     /coded_by="XM_034334438.2:108..977"
                     /db_xref="GeneID:117608809"
ORIGIN      
        1 mdfdtayiep lldgwledlq trikeqenci nvkdeyympf iaipvpvvsa ilkiteylel
       61 gpetkyvaih lydkfmcnyf wklykttnqt edhwsevckk issqaklylm sclqlaskld
      121 shsnnlgisq vlnvlkwidg kteytrgmif sseyevfkmv efkmplctpl ncvdvllaai
      181 glkntqnlqn lvislldlvy lqwemlysqq qylvyghvat thqekrncmt lkcnvlflaa
      241 svilcatffs cidneaakii asklsefvni npndiwkman ilfmmavqe
//
LOCUS       XP_034190331             160 aa            linear   INV 03-SEP-2025
DEFINITION  peroxiredoxin-5, mitochondrial-like [Osmia lignaria lignaria].
ACCESSION   XP_034190331
VERSION     XP_034190331.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334440.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..160
                     /product="peroxiredoxin-5, mitochondrial-like"
                     /calculated_mol_wt=17422
     Region          3..143
                     /region_name="AHP1"
                     /note="Peroxiredoxin [Posttranslational modification,
                     protein turnover, chaperones]; COG0678"
                     /db_xref="CDD:440442"
     CDS             1..160
                     /gene="LOC117608810"
                     /coded_by="XM_034334440.2:210..692"
                     /db_xref="GeneID:117608810"
ORIGIN      
        1 mpqirkgnri pnatlyeaqp enrinildlv gnkkaiifgv pgayvpgcsr vhlrgfieks
       61 tdlrflgfqe iicvsvndpf vlsawgnakg andkvrmlad pmasytkaig mdtdipelgg
      121 irsrrysmai vngvvkelfi dapnvklvcl hsdgvptyct
//
LOCUS       XP_034190290             242 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase MARCHF3-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190290
VERSION     XP_034190290.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334399.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="E3 ubiquitin-protein ligase MARCHF3-like isoform
                     X1"
                     /calculated_mol_wt=28672
     Region          58..107
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(58,61,76,81,84,87,100,103)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..242
                     /gene="LOC117608792"
                     /coded_by="XM_034334399.2:394..1122"
                     /db_xref="GeneID:117608792"
ORIGIN      
        1 mtnekssvec vlekiltkie lecreekrvi khaikrkidd ilvinkrrkl tlneeiqcri
       61 cydstqqlpi iypckckgtm gaihlkcleh wleesnrnsc elcghqyevr rtpryhvlys
      121 iiiwmclnqq qhqlyvrslk adlirgiiit pmaigcsyic ivaadfyann nydnlpparw
      181 ttylllimms lllfsyfiwm ymvieyhqkv wfywwqktst vtiilnpeni tsinyksnvs
      241 qv
//
LOCUS       XP_034190292             236 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608792 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190292
VERSION     XP_034190292.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334401.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="uncharacterized protein LOC117608792 isoform X2"
                     /calculated_mol_wt=26858
     CDS             1..236
                     /gene="LOC117608792"
                     /coded_by="XM_034334401.2:267..977"
                     /db_xref="GeneID:117608792"
ORIGIN      
        1 mlegwycrsi annnadaedd dddspeeevp nykdqyrnlk rklkfliyen ecfqealrst
       61 qrkllkvnrd ksflldrllq yekvdasfse sdetessdee vtrldtskrk kiemsisnht
      121 phhmptvakp lntskkkksa pkvtksnnip ivqsannvvp mplmsdghmt peeverhles
      181 rqtylelvpe kapptvptem fsndpsldse sneigelets psnmgedcls vdmmae
//
LOCUS       XP_034190293             236 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608792 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190293
VERSION     XP_034190293.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334402.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="uncharacterized protein LOC117608792 isoform X2"
                     /calculated_mol_wt=26858
     CDS             1..236
                     /gene="LOC117608792"
                     /coded_by="XM_034334402.2:309..1019"
                     /db_xref="GeneID:117608792"
ORIGIN      
        1 mlegwycrsi annnadaedd dddspeeevp nykdqyrnlk rklkfliyen ecfqealrst
       61 qrkllkvnrd ksflldrllq yekvdasfse sdetessdee vtrldtskrk kiemsisnht
      121 phhmptvakp lntskkkksa pkvtksnnip ivqsannvvp mplmsdghmt peeverhles
      181 rqtylelvpe kapptvptem fsndpsldse sneigelets psnmgedcls vdmmae
//
LOCUS       XP_034190294             216 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608792 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190294
VERSION     XP_034190294.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334403.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..216
                     /product="uncharacterized protein LOC117608792 isoform X3"
                     /calculated_mol_wt=24883
     CDS             1..216
                     /gene="LOC117608792"
                     /coded_by="XM_034334403.2:269..919"
                     /db_xref="GeneID:117608792"
ORIGIN      
        1 mlegwycrsi annnadaedd dddspeeevp nykdqyrnlk rklkfliyen ecfqealrst
       61 qrkllkvnrd ksflldrllq yekvdasfse sdetessdee vtrldtskrk kiemsisnht
      121 phhmptvakp lntskkkksa pkvtksnnip ivqsannvvp mplmsdghmt peeverhles
      181 rqtylelvpe kapptvptem fsndpsldre pwvrst
//
LOCUS       XP_034184679            1424 aa            linear   INV 03-SEP-2025
DEFINITION  THO complex subunit 2-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034184679
VERSION     XP_034184679.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328788.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184679.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1424
                     /product="THO complex subunit 2-like protein"
                     /calculated_mol_wt=164611
     Region          42..543
                     /region_name="THOC2_N"
                     /note="THO complex subunit 2 N-terminus; pfam16134"
                     /db_xref="CDD:465032"
     Region          545..619
                     /region_name="Thoc2"
                     /note="Transcription- and export-related complex subunit;
                     pfam11732"
                     /db_xref="CDD:463334"
     Region          854..1155
                     /region_name="Tho2"
                     /note="Transcription factor/nuclear export subunit protein
                     2; pfam11262"
                     /db_xref="CDD:463251"
     Region          <1127..1423
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1424
                     /gene="tho2"
                     /coded_by="XM_034328788.2:106..4380"
                     /db_xref="GeneID:117606404"
ORIGIN      
        1 magkmwnsei wkawdkhgkn dflklfkhkf kegntaewrr glyelitngi rgnikkdsvv
       61 qtlgelmnid gaipsavvdi ftlidaealn dernnfyyiv kesekfltdr ilkerleiet
      121 lqdvgtlknk nfqtkfikvk tklyykqrkf nlfreesegy sklivelnqe rpenevasil
      181 eivksligyf nldpnrvldi lletfenrpq ddaffiplir symsdqqvlc evlgfkycsm
      241 vnstpfslqk vtalmlqhgv iklddilpwl ipddstiike heqamkqake yvrklsvist
      301 kdkeevqeek enpqdkyasn qkfglceall eigawdvaqn lfsrlpehsl tdqrpialal
      361 ckmiqsliep vyrkhciisq klqgrrvppl ksslapkpie nlenihdkll pmlivlgpnl
      421 hhdpvlmyki mrlchavikq cpldsnkqpv dknnnlyydv ltildvallp slsfmdcncc
      481 vaeelwnilk yypyqnrycl yarwkndtpl qhaallrkra daqkkiksim krvsketikp
      541 vgrsigklth sspgvlfdyv liqiqlydnl igpvvdslky ltnisydvlg yclvealaga
      601 drdrfkhdgt sislwlqsla sfcgaifkky nieltgllqy vanqlkaqks ldllilkeiv
      661 qkmagieaae emtsdqlnam aggdllknea gyfsqvrntk kssqrlkeal aehdlavalc
      721 llmaqqkhcv vyretdkshl klvgklydqc qdtlvqfgtf lgstmtvdey vdrlpsihsm
      781 lqdnhihsdv afflarpmfa hainikydal rkadpnykkm stamkqakya eaaqavmapv
      841 aqsvrplhpl kvwedispqf lvtfwslsmy dlyvpvesyq reinklkqfa aqsadskdmt
      901 vskgkkeqer yttlieklqd errkqeehve kvfaylrqek dtwflsrsak saknetitqf
      961 lqlclfprct fttvdamyca kfvhtihslk tanfstllcy drlfcditys vtsctenean
     1021 rygrflcaml etvmrwhsek aifdkecsny pgfvtkfrvs nqfseandmv gfenyrhvch
     1081 kwhykitkai vvcldskdyv qirnsliili kilphfpvla klsqilerkv ekvreeergq
     1141 rqdlhvlats ysgqlkaktp nmireadfhh vgdktgktqd apnneasekv gdgisnkeit
     1201 ngdtrieken kdqrekrtas nqlhhessek irkkeenkes seskdkhikk eevkeddsmd
     1261 kkdrkyykee hyyatgvdnl drdlssvsns sassgptqdg sdrdakrrkv esvqkegrra
     1321 dgnldkkers skskirdeqk elrrekklgr krdradesav ateqkrrkdd erakgthqng
     1381 dipehrekhh ynkekspytk ertheregre srdkhrrssd pkrr
//
LOCUS       XP_034184680             881 aa            linear   INV 03-SEP-2025
DEFINITION  DNA repair protein complementing XP-C cells homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034184680
VERSION     XP_034184680.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328789.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184680.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="DNA repair protein complementing XP-C cells
                     homolog"
                     /calculated_mol_wt=99759
     Region          173..855
                     /region_name="rad4"
                     /note="DNA repair protein rad4; TIGR00605"
                     /db_xref="CDD:273170"
     CDS             1..881
                     /gene="Xpc"
                     /coded_by="XM_034328789.2:149..2794"
                     /db_xref="GeneID:117606405"
ORIGIN      
        1 mnglseddts dssdehfidl kksnsnsres pkpnvtssds gtedestgdv dfndvdqasn
       61 ielftqvlkn leysqklein dektekepst snyllppkne gkiqklktep qdtsfneite
      121 lllqgessta saskkklvnt pvktedaeap teytipkdgv kitlpgtslv fnkkkkkggq
      181 dikailrrrl rasqilvekv gllcwlaygf hfnkqinqpe imaavlslis tsnypkkrid
      241 lvylekftkw fkhifsfevv etngnkcink esllkvieek kisdykelvl lyivalralg
      301 lncrlvislc pphrvysdsp lfqndskgqq tksksksstg kegssskkgk ksakketdtt
      361 nvvqnspeak knanieakrk aaeilrskpq kstkknkdsk tnneaattsg nkskgskdes
      421 etveqgksgi sfkqlrsrkv navpkdkaia etdgtkskyy ieedstdset efqptpkrsk
      481 nnsnkkevtk tvksnkrnvt kllssdsete epdkakksqd iwaeiylese eswicasvmd
      541 ekihcvtdiy krakkpvlyi vawnsenlik dvtrrycpqw ltvtrkqrid ekwwletlsh
      601 wkekdtaisr aedemllqke leqplpktig eckghplyvi arhllkfeal yppdcvplgh
      661 lgtgdaiysr hcvhtlrsre twlkqarvvk pkqeaykivk srpkydklsr mvikdsalel
      721 fgewqtteye ppeakdgivp rneygnvdlf kqsmlpkgtv hvnlpglnri arklnidcap
      781 avvgfnfgcm gavpaiegyv vcsehedtlr eaweeeqvea tkrakekqek rvygnwkkli
      841 hgllirerla akyefskeek svaankrpkt krtdvkkakv s
//
LOCUS       XP_034184681             432 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606407 [Osmia lignaria lignaria].
ACCESSION   XP_034184681
VERSION     XP_034184681.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328790.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..432
                     /product="uncharacterized protein LOC117606407"
                     /calculated_mol_wt=50788
     Region          54..80
                     /region_name="LisH_2"
                     /note="pfam16045"
                     /db_xref="CDD:464992"
     Region          <179..>422
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..432
                     /gene="LOC117606407"
                     /coded_by="XM_034328790.2:226..1524"
                     /db_xref="GeneID:117606407"
ORIGIN      
        1 mdqeyfsnmy qwfekcgvis nirtqlrqnl inalkskdft lknngpksak qyiydlliae
       61 yllnhnyayt lsvfaseapl lidfskktvq csdenekcnk eklrsdyvsh aletlginph
      121 dpkgqyvisq yiendmplll cilkcitmfs ynlhndvpik ekiilcneht qtefswqsyn
      181 ayiekltalk kkliihkqtv dhklheremm lkeqailieq qleilnkklq evqnimhsls
      241 lkekhlkekk qskehqifqk emeltlkerl llqeaerlqe eqdkrnkleq elrklqeqkr
      301 mqpepsvdkv snlifkniev qtddindiiq kdkikvltke keeltalvqd qqskieqitq
      361 ralqlsrqve girslrpisv evptqtvntn tvisessste dilqdaklrl krleeeslka
      421 dqyyynfinn sp
//
LOCUS       XP_034185612             579 aa            linear   INV 03-SEP-2025
DEFINITION  ran-binding protein M [Osmia lignaria lignaria].
ACCESSION   XP_034185612
VERSION     XP_034185612.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329721.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..579
                     /product="ran-binding protein M"
                     /calculated_mol_wt=63849
     Region          65..207
                     /region_name="SPRY_RanBP9_10"
                     /note="SPRY domain in Ran binding proteins 9 and 10;
                     cd12909"
                     /db_xref="CDD:293966"
     Region          246..272
                     /region_name="LisH"
                     /note="Lissencephaly type-1-like homology motif;
                     smart00667"
                     /db_xref="CDD:128913"
     Region          280..552
                     /region_name="CTLH"
                     /note="CTLH/CRA C-terminal to LisH motif domain;
                     pfam10607"
                     /db_xref="CDD:402305"
     CDS             1..579
                     /gene="RanBPM"
                     /coded_by="XM_034329721.2:52..1791"
                     /db_xref="GeneID:117606803"
ORIGIN      
        1 maatseessv mepsqtnsgq nqpvdplkml ypmvneeetp lprswspkdk ynyiglsqnn
       61 lrvhykgygk thkdaasvrt thsipaacgl yyfevkivsk grdgymgigl sahgvnvnrl
      121 pgwdkhsygy hgddgnsfcs sgtgqpygpt fttgdvigcg vnlvdntafy tknghhlgia
      181 ftdlppnlyp tvglqtpgev vdanfgqepf vfdiddmlne lrvktrlqii nyptpdhgqg
      241 qwqavlhkmv stylvhhgyc ataeafanst gqgfeedlns iknrqkilkl vlagrmgeai
      301 eltsrlypgl lerdpnllfa lkcrqfvemv ngsdseisqn sninqtsviq stkaytksst
      361 ngnveemnmn naingsteql lvngqiendv dmeenvmngv kssnengyqn gdlntngykc
      421 qngedvdmet nslnqiqqqq nggcilentl nksnkkqlcg gdkqaiekml efgrqlysqs
      481 ihlrqqhgkn esnkkmlqda fsllayanpw nspvgwqldp qqretvcarl nsailessnl
      541 prrpplevaa sharelvrlm shaglgacgf avvdniiqy
//
LOCUS       XP_034184827            1106 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184827
VERSION     XP_034184827.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328936.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1106
                     /product="sorting nexin-13 isoform X1"
                     /calculated_mol_wt=126786
     Region          102..339
                     /region_name="PXA"
                     /note="PXA domain; pfam02194"
                     /db_xref="CDD:460484"
     Region          448..590
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          729..848
                     /region_name="PX_SNX13"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexin 13; cd06873"
                     /db_xref="CDD:132783"
     Site            order(774..776,800..801,814)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132783"
     Region          951..1061
                     /region_name="Nexin_C"
                     /note="Sorting nexin C terminal; pfam08628"
                     /db_xref="CDD:462541"
     CDS             1..1106
                     /gene="LOC117606475"
                     /coded_by="XM_034328936.2:150..3470"
                     /db_xref="GeneID:117606475"
ORIGIN      
        1 msgclsknpk mnvplygflg vaatllvylv gfgtivklfi cllalilgti tcvyrtysan
       61 lddviksere dlnkktekfr qyildvskqk mtftldkrit gsriideslq eildfvirdy
      121 vepwynvitd deefiysvrd taqkiainia nrvkgvdwip ylttrlvddv ashvrlyrqa
      181 rarikqvrsk klqkgsasss tssgtpkrtp thrrnksetd vswysqtkfy ipnlssltep
      241 ieaseekede slekiffdle vqmennlisr dtvctnstqe leflgeisei llylvlpkgd
      301 fdcltvrfil rellvnviir plldlfsdpd yinqaciwlc akegglpsdv fltvirvtds
      361 ldeltatksi vckeiahlrs kdsggdddls vkqqlnslly vkkiletrii gmqegldset
      421 dgigaqpdwn rllipgqklv nlpldellkn nialsyfidy mtsinaesyl yfylnvygwr
      481 vsaeqqisdi elqklhmsqa atsimgtrrk nndlenlkea atkiyqqyls ekaspklqld
      541 dalvktllnr iriepvketw fddlrtccye klqnedrflp gfkrsmayvk llaeldllkd
      601 ptseddsksl dsisisstnn eletldeise mckpdetrgt elkdsrfrsn sstslasive
      661 pnegrgtdet dsemniraik smrkassidv dqinegkdvs fylesnaeqf vkldentydr
      721 naikklqqgr fvitakiiet givndrgkty giyavavtks ydsgyqekwh iyrrysdfyd
      781 lhqkikekyy dlakipfpak kafhnmertv lerrmlmlns wmyqltkpav vdghmglqnl
      841 llsfleqgdy dkgvtggqis ktidtlmhpl ktsmktvtqa vktmpdnmln tvdgvmdnis
      901 kffgnpkkpn sfyentkvga gldvetddni plrimlllmd eifdlkvrnq wlrrrivtll
      961 rqiirtmfgd ivnrriveyv slltsparva gylklfknsf wpngvkaekk pardaemknr
     1021 trvaakvall sclsdelkhi igsettrrgl lrvfelfqrp vlnrrllyvl legiietlfp
     1081 qnnlveifrk lysvsprvqh rnmqkc
//
LOCUS       XP_034184828            1096 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-13 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184828
VERSION     XP_034184828.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328937.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1096
                     /product="sorting nexin-13 isoform X2"
                     /calculated_mol_wt=125740
     Region          92..329
                     /region_name="PXA"
                     /note="PXA domain; pfam02194"
                     /db_xref="CDD:460484"
     Region          438..580
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          719..838
                     /region_name="PX_SNX13"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexin 13; cd06873"
                     /db_xref="CDD:132783"
     Site            order(764..766,790..791,804)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132783"
     Region          941..1051
                     /region_name="Nexin_C"
                     /note="Sorting nexin C terminal; pfam08628"
                     /db_xref="CDD:462541"
     CDS             1..1096
                     /gene="LOC117606475"
                     /coded_by="XM_034328937.2:420..3710"
                     /db_xref="GeneID:117606475"
ORIGIN      
        1 mnvplygflg vaatllvylv gfgtivklfi cllalilgti tcvyrtysan lddviksere
       61 dlnkktekfr qyildvskqk mtftldkrit gsriideslq eildfvirdy vepwynvitd
      121 deefiysvrd taqkiainia nrvkgvdwip ylttrlvddv ashvrlyrqa rarikqvrsk
      181 klqkgsasss tssgtpkrtp thrrnksetd vswysqtkfy ipnlssltep ieaseekede
      241 slekiffdle vqmennlisr dtvctnstqe leflgeisei llylvlpkgd fdcltvrfil
      301 rellvnviir plldlfsdpd yinqaciwlc akegglpsdv fltvirvtds ldeltatksi
      361 vckeiahlrs kdsggdddls vkqqlnslly vkkiletrii gmqegldset dgigaqpdwn
      421 rllipgqklv nlpldellkn nialsyfidy mtsinaesyl yfylnvygwr vsaeqqisdi
      481 elqklhmsqa atsimgtrrk nndlenlkea atkiyqqyls ekaspklqld dalvktllnr
      541 iriepvketw fddlrtccye klqnedrflp gfkrsmayvk llaeldllkd ptseddsksl
      601 dsisisstnn eletldeise mckpdetrgt elkdsrfrsn sstslasive pnegrgtdet
      661 dsemniraik smrkassidv dqinegkdvs fylesnaeqf vkldentydr naikklqqgr
      721 fvitakiiet givndrgkty giyavavtks ydsgyqekwh iyrrysdfyd lhqkikekyy
      781 dlakipfpak kafhnmertv lerrmlmlns wmyqltkpav vdghmglqnl llsfleqgdy
      841 dkgvtggqis ktidtlmhpl ktsmktvtqa vktmpdnmln tvdgvmdnis kffgnpkkpn
      901 sfyentkvga gldvetddni plrimlllmd eifdlkvrnq wlrrrivtll rqiirtmfgd
      961 ivnrriveyv slltsparva gylklfknsf wpngvkaekk pardaemknr trvaakvall
     1021 sclsdelkhi igsettrrgl lrvfelfqrp vlnrrllyvl legiietlfp qnnlveifrk
     1081 lysvsprvqh rnmqkc
//
LOCUS       XP_034184829             927 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-13 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034184829
VERSION     XP_034184829.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328938.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..927
                     /product="sorting nexin-13 isoform X3"
                     /calculated_mol_wt=105699
     Region          102..339
                     /region_name="PXA"
                     /note="PXA domain; pfam02194"
                     /db_xref="CDD:460484"
     Region          448..590
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          729..848
                     /region_name="PX_SNX13"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexin 13; cd06873"
                     /db_xref="CDD:132783"
     Site            order(774..776,800..801,814)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132783"
     CDS             1..927
                     /gene="LOC117606475"
                     /coded_by="XM_034328938.2:152..2935"
                     /db_xref="GeneID:117606475"
ORIGIN      
        1 msgclsknpk mnvplygflg vaatllvylv gfgtivklfi cllalilgti tcvyrtysan
       61 lddviksere dlnkktekfr qyildvskqk mtftldkrit gsriideslq eildfvirdy
      121 vepwynvitd deefiysvrd taqkiainia nrvkgvdwip ylttrlvddv ashvrlyrqa
      181 rarikqvrsk klqkgsasss tssgtpkrtp thrrnksetd vswysqtkfy ipnlssltep
      241 ieaseekede slekiffdle vqmennlisr dtvctnstqe leflgeisei llylvlpkgd
      301 fdcltvrfil rellvnviir plldlfsdpd yinqaciwlc akegglpsdv fltvirvtds
      361 ldeltatksi vckeiahlrs kdsggdddls vkqqlnslly vkkiletrii gmqegldset
      421 dgigaqpdwn rllipgqklv nlpldellkn nialsyfidy mtsinaesyl yfylnvygwr
      481 vsaeqqisdi elqklhmsqa atsimgtrrk nndlenlkea atkiyqqyls ekaspklqld
      541 dalvktllnr iriepvketw fddlrtccye klqnedrflp gfkrsmayvk llaeldllkd
      601 ptseddsksl dsisisstnn eletldeise mckpdetrgt elkdsrfrsn sstslasive
      661 pnegrgtdet dsemniraik smrkassidv dqinegkdvs fylesnaeqf vkldentydr
      721 naikklqqgr fvitakiiet givndrgkty giyavavtks ydsgyqekwh iyrrysdfyd
      781 lhqkikekyy dlakipfpak kafhnmertv lerrmlmlns wmyqltkpav vdghmglqnl
      841 llsfleqgdy dkgvtggqis ktidtlmhpl ktsmktvtqa vktmpdnmln tvdgvmdnis
      901 kffgnpkkpn sfyentkvga gldvevd
//
LOCUS       XP_076545526             328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610919 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545526
VERSION     XP_076545526.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689411.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="uncharacterized protein LOC117610919 isoform X1"
                     /calculated_mol_wt=37672
     Region          195..>254
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..328
                     /gene="LOC117610919"
                     /coded_by="XM_076689411.1:32..1018"
                     /db_xref="GeneID:117610919"
ORIGIN      
        1 mytyfkfssn illlqmemeh rpkrmankpa ryqttssdee prkrlkvapg siveditdlr
       61 atlegvddev pshnyrqheh tstytpldty spytpsnini ysphippnty rppptvyapp
      121 tqtfhipdav esrpeprmqq gteptnvqir dlytkignle smvtkthsll ekvakqqqql
      181 lhqrhgipsk paflpissla amtefqntde dsyrevvnyl eyvggfnlke avtlsfkeav
      241 sdclltsysw lgrednnlal cetkivhaih davlqnryfp rpsrkdvaeh lkaalrtake
      301 rvrskrrgpr npqhetvdnf wtakeqpr
//
LOCUS       XP_076545527             328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610919 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545527
VERSION     XP_076545527.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689412.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="uncharacterized protein LOC117610919 isoform X1"
                     /calculated_mol_wt=37672
     Region          195..>254
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..328
                     /gene="LOC117610919"
                     /coded_by="XM_076689412.1:32..1018"
                     /db_xref="GeneID:117610919"
ORIGIN      
        1 mytyfkfssn illlqmemeh rpkrmankpa ryqttssdee prkrlkvapg siveditdlr
       61 atlegvddev pshnyrqheh tstytpldty spytpsnini ysphippnty rppptvyapp
      121 tqtfhipdav esrpeprmqq gteptnvqir dlytkignle smvtkthsll ekvakqqqql
      181 lhqrhgipsk paflpissla amtefqntde dsyrevvnyl eyvggfnlke avtlsfkeav
      241 sdclltsysw lgrednnlal cetkivhaih davlqnryfp rpsrkdvaeh lkaalrtake
      301 rvrskrrgpr npqhetvdnf wtakeqpr
//
LOCUS       XP_076545528             317 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610919 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545528
VERSION     XP_076545528.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689413.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="uncharacterized protein LOC117610919 isoform X2"
                     /calculated_mol_wt=36268
     Region          184..>243
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..317
                     /gene="LOC117610919"
                     /coded_by="XM_076689413.1:149..1102"
                     /db_xref="GeneID:117610919"
ORIGIN      
        1 mnsnmemehr pkrmankpar yqttssdeep rkrlkvapgs iveditdlra tlegvddevp
       61 shnyrqheht stytpldtys pytpsniniy sphippntyr ppptvyappt qtfhipdave
      121 srpeprmqqg teptnvqird lytkignles mvtkthslle kvakqqqqll hqrhgipskp
      181 aflpisslaa mtefqntded syrevvnyle yvggfnlkea vtlsfkeavs dclltsyswl
      241 grednnlalc etkivhaihd avlqnryfpr psrkdvaehl kaalrtaker vrskrrgprn
      301 pqhetvdnfw takeqpr
//
LOCUS       XP_076545529             165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305497 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545529
VERSION     XP_076545529.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689414.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..165
                     /product="uncharacterized protein LOC143305497 isoform X1"
                     /calculated_mol_wt=18955
     CDS             1..165
                     /gene="LOC143305497"
                     /coded_by="XM_076689414.1:774..1271"
                     /db_xref="GeneID:143305497"
ORIGIN      
        1 mwkrklqadr twrtllpsts rpwassnsaa carietssev ansvlvfree gfieessclv
       61 dvtlcgnenc kqiepgeryy pqpadhghlr illlvrvsrq vlklrircpf fakkaslknh
      121 rfwstflyve tktanrsnle nvtalnqqtm gifefcclca yrdkf
//
LOCUS       XP_076545530             165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305497 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545530
VERSION     XP_076545530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689415.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..165
                     /product="uncharacterized protein LOC143305497 isoform X2"
                     /calculated_mol_wt=18796
     CDS             1..165
                     /gene="LOC143305497"
                     /coded_by="XM_076689415.1:583..1080"
                     /db_xref="GeneID:143305497"
ORIGIN      
        1 mwkrklqadr twrtllpsts rpwassnsva carietssev anlvpvfree gfieessflv
       61 dvtlcgnenc kqiepggryc pqpadhghlr illlvrvsrq vlklrircsf sakkvslknh
      121 rvwstllyve tktasrsnle nvitlnqqtm gifefcclca yrdkf
//
LOCUS       XP_076545531             152 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305497 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545531
VERSION     XP_076545531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689416.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..152
                     /product="uncharacterized protein LOC143305497 isoform X3"
                     /calculated_mol_wt=17228
     CDS             1..152
                     /gene="LOC143305497"
                     /coded_by="XM_076689416.1:1443..1901"
                     /db_xref="GeneID:143305497"
ORIGIN      
        1 mylqgvcpei rniekvacar ietssevanl vpvfreegfi eessflvdvt lcgnenckqi
       61 epggrycpqp adhghlrill lvrvsrqvlk lrircsfsak kvslknhrvw stllyvetkt
      121 asrsnlenvi tlnqqtmgif efcclcayrd kf
//
LOCUS       XP_076545532              98 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305497 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545532
VERSION     XP_076545532.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689417.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..98
                     /product="uncharacterized protein LOC143305497 isoform X4"
                     /calculated_mol_wt=10920
     CDS             1..98
                     /gene="LOC143305497"
                     /coded_by="XM_076689417.1:581..877"
                     /db_xref="GeneID:143305497"
ORIGIN      
        1 mwkrklqadr twrtllpsts rpwassnsva carietssev anlvpvfree gfieessflv
       61 dvtlcgnenc kqiepggryc pqpadhghlr ilihdape
//
LOCUS       XP_076545533              95 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305497 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545533
VERSION     XP_076545533.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689418.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..95
                     /product="uncharacterized protein LOC143305497 isoform X5"
                     /calculated_mol_wt=10570
     CDS             1..95
                     /gene="LOC143305497"
                     /coded_by="XM_076689418.1:579..866"
                     /db_xref="GeneID:143305497"
ORIGIN      
        1 mwkrklqadr twrtllpsts rpwassnsva carietssev anlvpvfree gfieessflv
       61 dvtlcgnenc kqiepggryc pqpadhghlr ilpnt
//
LOCUS       XP_076545534             117 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610920 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545534
VERSION     XP_076545534.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689419.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..117
                     /product="uncharacterized protein LOC117610920 isoform X1"
                     /calculated_mol_wt=13291
     CDS             1..117
                     /gene="LOC117610920"
                     /coded_by="XM_076689419.1:609..962"
                     /db_xref="GeneID:117610920"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lprfynicrn tspwsiqdsp
       61 nilrevgkag mgpkkvsgta selqnteedk eeeeeeeeee relsststpw masippa
//
LOCUS       XP_076545535             115 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610920 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545535
VERSION     XP_076545535.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689420.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="uncharacterized protein LOC117610920 isoform X2"
                     /calculated_mol_wt=13105
     CDS             1..115
                     /gene="LOC117610920"
                     /coded_by="XM_076689420.1:609..956"
                     /db_xref="GeneID:117610920"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lprfynicrn tspwsiqdsp
       61 nilrevgkag mgpkkgtase lqnteedkee eeeeeeeere lsststpwma sippa
//
LOCUS       XP_076545536             111 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610920 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545536
VERSION     XP_076545536.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689421.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..111
                     /product="uncharacterized protein LOC117610920 isoform X3"
                     /calculated_mol_wt=12692
     CDS             1..111
                     /gene="LOC117610920"
                     /coded_by="XM_076689421.1:611..946"
                     /db_xref="GeneID:117610920"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lprfynicrn tspwsiqdsp
       61 nilrevgkav sgtaselqnt eedkeeeeee eeeerelsst stpwmasipp a
//
LOCUS       XP_076545537             109 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610920 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545537
VERSION     XP_076545537.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689422.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..109
                     /product="uncharacterized protein LOC117610920 isoform X4"
                     /calculated_mol_wt=12506
     CDS             1..109
                     /gene="LOC117610920"
                     /coded_by="XM_076689422.1:612..941"
                     /db_xref="GeneID:117610920"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lprfynicrn tspwsiqdsp
       61 nilrevgkag taselqntee dkeeeeeeee eerelsstst pwmasippa
//
LOCUS       XP_034184690            1373 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606413 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184690
VERSION     XP_034184690.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328799.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184690.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1373
                     /product="uncharacterized protein LOC117606413 isoform X1"
                     /calculated_mol_wt=150220
     Region          <990..1115
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1373
                     /gene="LOC117606413"
                     /coded_by="XM_034328799.2:490..4611"
                     /db_xref="GeneID:117606413"
ORIGIN      
        1 mawtgtqrwr npapegddvq rsielldkvl seydeheteg eggggvvgvg vsvsvgvgvg
       61 igvgsggngg gtgtgvgdcg sstepniglt pddespslgh qseddgymsm ngrkakmalv
      121 alrpvpdcpe pqdlvgisrq qfppppeeae riistllpmv spgnsskrtg rsadrrngrh
      181 qwmmamqdsi rqgngaaitt qttlpktrhq rpygwenghg seplklahpp sppskfaslp
      241 ydgkisfgwi pprtnpttve khrevkrrss eeeeeeeden gverekkrfe kdrashlrkq
      301 rqnnemtkss svedrllmnh qrnvdenegn drndrtrtds dssegrfsrn seserssipr
      361 sssvsverfs mratcdssdf sranstsner fhsdfsiava sassndrvsm ptsdpfsirn
      421 ldfvsyslpn radsserfsa ptsdryseer ysdmfstrns dfstrnstdf rtqseeerfs
      481 ddsleellpp ppppppppis krhsiawevp leddplyapg stkvigrrrr kssdvssvgs
      541 asklrdldde wqdprlsttd ddlvspysds stneldqirp eaftkngtyv irrgrkkerk
      601 glpkapsktk slsfentekn rlsevkryss tfdniksllr ekkleatndh ppnqvefass
      661 llppdlvrvi slpaindqea dddrgqlevt veeeetsdld sdkdkkrree vveqlarfss
      721 sgfaasfsls lslslsttvd dendrnqenv qrigttcngg tslsasnsys kgfareeqps
      781 akkssncssl avlgsehrla skipvnllie dqivkkalke nrrqlekvsd aikeiesvkn
      841 sesysenkrw kttdgkhptk rnsleidqne rqsiddvnlq aeearakkqr dsdnsvdsda
      901 dtdadadada skaspktenf dgrrangtdv hssgrrsrqk tsavnaavav avavenhrnd
      961 qkeienvida ilqdskntdf qvsvevlefp plppspveea deessdigqt gltapkaksh
     1021 nnrtmeyrpr vpphraapla sssdskdhfq tslntrsmda gfsrgrrstp tagnstrreq
     1081 ipverrtlpt dlpgpsrrrp ftkrhspste pcassdgsat ataianghgs gngngngngn
     1141 gngngngssn gacssgggng gtsttnnvat gsgmqtscsl petpvfargs diprtpqhan
     1201 nstqmrrqpg wyapttattg yrrnnpgleq aiigtellrl aggpsrgwyp trkanqprpa
     1261 siehlerlnn aydarlvgtg dqrkpltlpt nitpnkyfgq skhssasstr ealrrvtsll
     1321 ikkgsgskds kggkeggssc gpyaaaecgd apkkkgffkg fwkrsrhysl enq
//
LOCUS       XP_034184691            1350 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606413 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184691
VERSION     XP_034184691.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328800.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184691.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1350
                     /product="uncharacterized protein LOC117606413 isoform X2"
                     /calculated_mol_wt=147781
     Region          <990..1115
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1350
                     /gene="LOC117606413"
                     /coded_by="XM_034328800.2:490..4542"
                     /db_xref="GeneID:117606413"
ORIGIN      
        1 mawtgtqrwr npapegddvq rsielldkvl seydeheteg eggggvvgvg vsvsvgvgvg
       61 igvgsggngg gtgtgvgdcg sstepniglt pddespslgh qseddgymsm ngrkakmalv
      121 alrpvpdcpe pqdlvgisrq qfppppeeae riistllpmv spgnsskrtg rsadrrngrh
      181 qwmmamqdsi rqgngaaitt qttlpktrhq rpygwenghg seplklahpp sppskfaslp
      241 ydgkisfgwi pprtnpttve khrevkrrss eeeeeeeden gverekkrfe kdrashlrkq
      301 rqnnemtkss svedrllmnh qrnvdenegn drndrtrtds dssegrfsrn seserssipr
      361 sssvsverfs mratcdssdf sranstsner fhsdfsiava sassndrvsm ptsdpfsirn
      421 ldfvsyslpn radsserfsa ptsdryseer ysdmfstrns dfstrnstdf rtqseeerfs
      481 ddsleellpp ppppppppis krhsiawevp leddplyapg stkvigrrrr kssdvssvgs
      541 asklrdldde wqdprlsttd ddlvspysds stneldqirp eaftkngtyv irrgrkkerk
      601 glpkapsktk slsfentekn rlsevkryss tfdniksllr ekkleatndh ppnqvefass
      661 llppdlvrvi slpaindqea dddrgqlevt veeeetsdld sdkdkkrree vveqlarfss
      721 sgfaasfsls lslslsttvd dendrnqenv qrigttcngg tslsasnsys kgfareeqps
      781 akkssncssl avlgsehrla skipvnllie dqivkkalke nrrqlekvsd aikeiesvkn
      841 sesysenkrw kttdgkhptk rnsleidqne rqsiddvnlq aeearakkqr dsdnsvdsda
      901 dtdadadada skaspktenf dgrrangtdv hssgrrsrqk tsavnaavav avavenhrnd
      961 qkeienvida ilqdskntdf qvsvevlefp plppspveea deessdigqt gltapkaksh
     1021 nnrtmeyrpr vpphraapla sssdskdhfq tslntrsmda gfsrgrrstp tagnstrreq
     1081 ipverrtlpt dlpgpsrrrp ftkrhspste pcassdgsat ataianghgs gngngngngn
     1141 gngngngssn gacssgggng gtsttnnvat gsgmqtscsl petpvfargs diprtpqhan
     1201 nstqmrrqpg wyapttattg yrrnnpgleq aiigtellrl aggpsrgwyp trkanqprpa
     1261 siehlerlnn aydarlvgtg dqrkpltlpt nitpnkyfgq rsgskdskgg keggsscgpy
     1321 aaaecgdapk kkgffkgfwk rsrhyslenq
//
LOCUS       XP_034184692            1266 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606413 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184692
VERSION     XP_034184692.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328801.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184692.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1266
                     /product="uncharacterized protein LOC117606413 isoform X3"
                     /calculated_mol_wt=139577
     Region          <883..1008
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1266
                     /gene="LOC117606413"
                     /coded_by="XM_034328801.2:560..4360"
                     /db_xref="GeneID:117606413"
ORIGIN      
        1 msmngrkakm alvalrpvpd cpepqdlvgi srqqfppppe eaeriistll pmvspgnssk
       61 rtgrsadrrn grhqwmmamq dsirqgngaa ittqttlpkt rhqrpygwen ghgseplkla
      121 hppsppskfa slpydgkisf gwipprtnpt tvekhrevkr rsseeeeeee dengverekk
      181 rfekdrashl rkqrqnnemt ksssvedrll mnhqrnvden egndrndrtr tdsdssegrf
      241 srnseserss iprsssvsve rfsmratcds sdfsransts nerfhsdfsi avasassndr
      301 vsmptsdpfs irnldfvsys lpnradsser fsaptsdrys eerysdmfst rnsdfstrns
      361 tdfrtqseee rfsddsleel lppppppppp piskrhsiaw evpleddply apgstkvigr
      421 rrrkssdvss vgsasklrdl ddewqdprls ttdddlvspy sdsstneldq irpeaftkng
      481 tyvirrgrkk erkglpkaps ktkslsfent eknrlsevkr ysstfdniks llrekkleat
      541 ndhppnqvef assllppdlv rvislpaind qeadddrgql evtveeeets dldsdkdkkr
      601 reevveqlar fsssgfaasf slslslslst tvddendrnq envqrigttc nggtslsasn
      661 syskgfaree qpsakkssnc sslavlgseh rlaskipvnl liedqivkka lkenrrqlek
      721 vsdaikeies vknsesysen krwkttdgkh ptkrnsleid qnerqsiddv nlqaeearak
      781 kqrdsdnsvd sdadtdadad adaskaspkt enfdgrrang tdvhssgrrs rqktsavnaa
      841 vavavavenh rndqkeienv idailqdskn tdfqvsvevl efpplppspv eeadeessdi
      901 gqtgltapka kshnnrtmey rprvpphraa plasssdskd hfqtslntrs mdagfsrgrr
      961 stptagnstr reqipverrt lptdlpgpsr rrpftkrhsp stepcassdg satataiang
     1021 hgsgngngng ngngngngng ssngacssgg gnggtsttnn vatgsgmqts cslpetpvfa
     1081 rgsdiprtpq hannstqmrr qpgwyaptta ttgyrrnnpg leqaiigtel lrlaggpsrg
     1141 wyptrkanqp rpasiehler lnnaydarlv gtgdqrkplt lptnitpnky fgqskhssas
     1201 strealrrvt sllikkgsgs kdskggkegg sscgpyaaae cgdapkkkgf fkgfwkrsrh
     1261 yslenq
//
LOCUS       XP_034184694            1132 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606413 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184694
VERSION     XP_034184694.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328803.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184694.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1132
                     /product="uncharacterized protein LOC117606413 isoform X4"
                     /calculated_mol_wt=125544
     CDS             1..1132
                     /gene="LOC117606413"
                     /coded_by="XM_034328803.2:490..3888"
                     /db_xref="GeneID:117606413"
ORIGIN      
        1 mawtgtqrwr npapegddvq rsielldkvl seydeheteg eggggvvgvg vsvsvgvgvg
       61 igvgsggngg gtgtgvgdcg sstepniglt pddespslgh qseddgymsm ngrkakmalv
      121 alrpvpdcpe pqdlvgisrq qfppppeeae riistllpmv spgnsskrtg rsadrrngrh
      181 qwmmamqdsi rqgngaaitt qttlpktrhq rpygwenghg seplklahpp sppskfaslp
      241 ydgkisfgwi pprtnpttve khrevkrrss eeeeeeeden gverekkrfe kdrashlrkq
      301 rqnnemtkss svedrllmnh qrnvdenegn drndrtrtds dssegrfsrn seserssipr
      361 sssvsverfs mratcdssdf sranstsner fhsdfsiava sassndrvsm ptsdpfsirn
      421 ldfvsyslpn radsserfsa ptsdryseer ysdmfstrns dfstrnstdf rtqseeerfs
      481 ddsleellpp ppppppppis krhsiawevp leddplyapg stkvigrrrr kssdvssvgs
      541 asklrdldde wqdprlsttd ddlvspysds stneldqirp eaftkngtyv irrgrkkerk
      601 glpkapsktk slsfentekn rlsevkryss tfdniksllr ekkleatndh ppnqvefass
      661 llppdlvrvi slpaindqea dddrgqlevt veeeetsdld sdkdkkrree vveqlarfss
      721 sgfaasfsls lslslsttvd dendrnqenv qrigttcngg tslsasnsys kgfareeqps
      781 akkssncssl avlgsehrla skipvnllie dqivkkalke nrrqlekvsd aikeiesvkn
      841 sesysenkrw kttdgkhptk rnsleidqne rqsiddvnlq aeearakkqr dsdnsvdsda
      901 dtdadadada skaspktenf dgrrangtdv hssgrrsrqk tsavnaavav avavenhrnd
      961 qkeienvida ilqdskntdf qvsvevlefp plppspveea deessdigqt gltapkaksh
     1021 nnrtmeyrpr vpphraapla sssdskdhfq tslntrsmda gfsrgrrstp tagnstrree
     1081 aeartarver radrravltp rpnaemrqrr kdfskvsgrd rvitlwrtns pg
//
LOCUS       XP_034184697            1028 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR3 isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184697
VERSION     XP_034184697.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328806.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184697.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1028
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR3 isoform X1"
                     /calculated_mol_wt=115775
     Region          31..140
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          162..506
                     /region_name="Myotub-related"
                     /note="Myotubularin-like phosphatase domain; pfam06602"
                     /db_xref="CDD:461958"
     Region          885..944
                     /region_name="FYVE_MTMR4"
                     /note="FYVE domain found in myotubularin-related protein 4
                     (MTMR4) and similar proteins; cd15733"
                     /db_xref="CDD:277272"
     Site            order(885,888,906..911,913..914,938..940)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277272"
     CDS             1..1028
                     /gene="LOC117606416"
                     /coded_by="XM_034328806.2:228..3314"
                     /db_xref="GeneID:117606416"
ORIGIN      
        1 mdnseeptsl qsichihase lfpkhahfec edqtltvpft plsgesival grtsdgilal
       61 snyrlylqls qtcyniplgl ieqlevkeif flhiackdar slscafstne hclewfkrlh
      121 kltyprknie difafayyaw sveegkdslk lgkdnasysg tfnseverlk fnlhgtwris
      181 qmnkdyrmcp sypplllvpa cisdkiletv akfrssrrip avvwrhvkng aviarssqpe
      241 vgwlgwrsae dedllkalad acsydkgest mvdssiiypd nsddgvagns skkvlivdar
      301 syttavanra rgggcecpey ypscdiqfmn lpnihsirks fhavrqlcas dadqpnwlsl
      361 legtrwlqhm sgllraavtv asaierdgrp vlvhcsdgwd rtpqivalaq illdpyyrtm
      421 egfqilcere wldfghkfad rcgqtvgced pnercpvflq wldcvhqlil qfkcsfqisp
      481 aylvklaqht ysqlfgtflc ntrqerlqlr irertfsvwr flnsssfvnh lysplknqvl
      541 wpscnvrdlv lwsevylgsm esslgirddk qrvtmidieg gdneepqgqm tktrsygdlm
      601 hapdhtaylh rrlsdpsisv ekkfdsltld teseekienq venckservd enrvekfdet
      661 etqyarvqvl ndspvnpsvd sstdtlipsn dslvgvmnvd ketkvvknsp sdmvspwien
      721 ntigrtvcsc srnynqnqnq nqnqnqnqnq negssdvsdt saplisrtps sicpaspthq
      781 datadnldrl ddadglpviq cdvqmrvqqi ivehklkeea lrkelhttrl alikqvcnhn
      841 adtervddig slpdsvgstg dhgeslpsdm sweaveelgp aptlwvpdha vsrcmgcdte
      901 fwlgrrkhhc rccgkifcad csenstplps eqlynpvrvc secfsrlhrh aspcqcnvrh
      961 qnkgendael ssnseslitc qrskglhltk dsccdnllqa ahqktqpppv taatvfptkr
     1021 randahhg
//
LOCUS       XP_034184698            1019 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-3,5-bisphosphate 3-phosphatase MTMR3 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184698
VERSION     XP_034184698.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328807.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184698.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1019
                     /product="phosphatidylinositol-3,5-bisphosphate
                     3-phosphatase MTMR3 isoform X2"
                     /calculated_mol_wt=114793
     Region          31..140
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          162..506
                     /region_name="Myotub-related"
                     /note="Myotubularin-like phosphatase domain; pfam06602"
                     /db_xref="CDD:461958"
     Region          885..944
                     /region_name="FYVE_MTMR4"
                     /note="FYVE domain found in myotubularin-related protein 4
                     (MTMR4) and similar proteins; cd15733"
                     /db_xref="CDD:277272"
     Site            order(885,888,906..911,913..914,938..940)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277272"
     CDS             1..1019
                     /gene="LOC117606416"
                     /coded_by="XM_034328807.2:228..3287"
                     /db_xref="GeneID:117606416"
ORIGIN      
        1 mdnseeptsl qsichihase lfpkhahfec edqtltvpft plsgesival grtsdgilal
       61 snyrlylqls qtcyniplgl ieqlevkeif flhiackdar slscafstne hclewfkrlh
      121 kltyprknie difafayyaw sveegkdslk lgkdnasysg tfnseverlk fnlhgtwris
      181 qmnkdyrmcp sypplllvpa cisdkiletv akfrssrrip avvwrhvkng aviarssqpe
      241 vgwlgwrsae dedllkalad acsydkgest mvdssiiypd nsddgvagns skkvlivdar
      301 syttavanra rgggcecpey ypscdiqfmn lpnihsirks fhavrqlcas dadqpnwlsl
      361 legtrwlqhm sgllraavtv asaierdgrp vlvhcsdgwd rtpqivalaq illdpyyrtm
      421 egfqilcere wldfghkfad rcgqtvgced pnercpvflq wldcvhqlil qfkcsfqisp
      481 aylvklaqht ysqlfgtflc ntrqerlqlr irertfsvwr flnsssfvnh lysplknqvl
      541 wpscnvrdlv lwsevylgsm esslgirddk qrvtmidieg gdneepqgqm tktrsygdlm
      601 hapdhtaylh rrlsdpsisv ekkfdsltld teseekienq venckservd enrvekfdet
      661 etqyarvqvl ndspvnpsvd sstdtlipsn dslvgvmnvd ketkvvknsp sdmvspwien
      721 ntigrtvcsc srnynqnqnq nqnqnqnqnq negssdvsdt saplisrtps sicpaspthq
      781 datadnldrl ddadglpviq cdvqmrvqqi ivehklkeea lrkelhttrl alikqvcnhn
      841 adtervddig slpdsvgstg dhgeslpsdm sweaveelgp aptlwvpdha vsrcmgcdte
      901 fwlgrrkhhc rccgkifcad csenstplps eqlynpvrvc secfsrlhrh aspcqcnvrh
      961 qnkgendael ssnseslitc qrskglhltk dsccdnllqa ahqktqpppv taatietvy
//
LOCUS       XP_034184713             749 aa            linear   INV 03-SEP-2025
DEFINITION  HMG-coA reductase degradation 3 [Osmia lignaria lignaria].
ACCESSION   XP_034184713
VERSION     XP_034184713.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328822.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..749
                     /product="HMG-coA reductase degradation 3"
                     /calculated_mol_wt=83364
     Region          119..380
                     /region_name="TPR"
                     /note="TPR repeat [General function prediction only];
                     COG0790"
                     /db_xref="CDD:440553"
     Region          166..195
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          210..239
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          247..276
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          334..358
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          <335..525
                     /region_name="TPR"
                     /note="TPR repeat [General function prediction only];
                     COG0790"
                     /db_xref="CDD:440553"
     Region          366..395
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          403..432
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          439..468
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          544..572
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          579..612
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          600..635
                     /region_name="SEL1"
                     /note="Sel1-like repeats; smart00671"
                     /db_xref="CDD:214772"
     Region          619..649
                     /region_name="SLR repeat"
                     /note="SLR repeat [structural motif]"
                     /db_xref="CDD:276807"
     Region          637..670
                     /region_name="Sel1"
                     /note="Sel1 repeat; pfam08238"
                     /db_xref="CDD:429881"
     CDS             1..749
                     /gene="Hrd3"
                     /coded_by="XM_034328822.2:309..2558"
                     /db_xref="GeneID:117606420"
ORIGIN      
        1 mklrkelill llvmsvsses evskkqsdts deeaeddvgl eenafehlqe lkalhetilk
       61 mvpasepyln dkitkrkeea vnepktkees ptmrnvwmka mitqiknidl pnnnlrleed
      121 daevwseeeq leplteetee pltpeqqeae qifrkaqsll natrsnkaea yelltsaail
      181 ghresrsmla waqllgtqfa ptfswissqd ipaayqtfke laetglpsah mglgflyatg
      241 lggvnasqpk allhytvaal ggdtraqmal gyrhwagvtt pvsceraldf yrkvankvae
      301 evslsggpvv qrvrlldeqe npgyssgifd qdlieyyqll akkgdtqaqv glgqlhyqgg
      361 rgvpldhera lqyfqhaada gnavamaflg kiylegsdiv kqdnetayky fkkaaelgnp
      421 vgqsglglmy lygrgverdt akalqyfnqa aeqgwvdgql qlgnmyfsgt gvrrdyklan
      481 kyfnlasqsg hvlafynlaq mhatgtgmmr scptavellk nvaergkwsd qlmiahtdyr
      541 egrineafvk yallaemgye vaqsnaafil dkgettilse eeglvralal waraaaqgys
      601 aaqvklgdah yygrgtkidy eaaashyrla seqqhnaqam fnlgymherg lglardrhla
      661 krcydlaaea spdaripval aliklsflfg ldylqefsli dhlnkwdqll gqnwdlylig
      721 lltgmlslii yfrrpqpppp pppppprin
//
LOCUS       XP_076545538             682 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolar protein 10 [Osmia lignaria lignaria].
ACCESSION   XP_076545538
VERSION     XP_076545538.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689423.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..682
                     /product="nucleolar protein 10"
                     /calculated_mol_wt=79342
     Region          <149..336
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          180..222
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          233..268
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          276..310
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          477..504
                     /region_name="NUC153"
                     /note="NUC153 domain; pfam08159"
                     /db_xref="CDD:462385"
     CDS             1..682
                     /gene="LOC117606469"
                     /coded_by="XM_076689423.1:490..2538"
                     /db_xref="GeneID:117606469"
ORIGIN      
        1 mqvscpnnvk iynlsagksl pewlserkrr nllkknvdir rrieliqdfd mpgvstsikv
       61 tkdeqyilat giykprvkcy dvnnlslkfe rcfdsevvtf dvlsddyskl vflhcdrnie
      121 fhvahgryyr lriprfgrdi kyhyptcdlf vvgdsneiyr inlergqflq sfsteassln
      181 kceinpvhhl lavgteegki eawdprsrsk agtldcalhc igqsdklqvi pavtslkfqg
      241 gltcgvgmsn gqillydirs nkpflikdhm yglpiksief hqkmdmvysm dssivkiwdk
      301 nngklytsie aendfndmcv ipntgmllia nenrkmqtyy vpslgtapyw csfldnltee
      361 leelnyeiiy ddykflteke ldelgllhlk gtnllraymh gyfmdirlyr kardamkpfe
      421 fqeykkkriq ekmqetcasr lqiekiatvn kelalklmde tntkkkktts ssgllkderf
      481 kalftnpdfq vdttseeysl lnpvvsqlnk srakrllkak ateeemkldp tndetqdnns
      541 sdesfihdds ssedekvwvk evkkqyrlmk knereiqeed esvkntdehr enepkfyeik
      601 envefkdakp vakrlnkasl gerlrdqeah niqvsgsrgn reitfvvekk kqtkrskgqv
      661 krhekgykhl lrpagnlfkk rr
//
LOCUS       XP_076545539             631 aa            linear   INV 03-SEP-2025
DEFINITION  protein I'm not dead yet isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545539
VERSION     XP_076545539.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689424.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="protein I'm not dead yet isoform X1"
                     /calculated_mol_wt=70083
     Region          <33..183
                     /region_name="ArsB_NhaD_permease"
                     /note="Anion permease ArsB/NhaD. These permeases have been
                     shown to translocate sodium, arsenate, antimonite, sulfate
                     and organic anions across biological membranes in all
                     three kingdoms of life. A typical anion permease contains
                     8-13 transmembrane helices...; cl21473"
                     /db_xref="CDD:473875"
     Region          114..604
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..631
                     /gene="LOC117606426"
                     /coded_by="XM_076689424.1:152..2047"
                     /db_xref="GeneID:117606426"
ORIGIN      
        1 mkqqarsrqe qeggegeeeh nkwwiltlrf lktywkiifa itwamflapf lfiynypevr
       61 caytillmag ywicecfpla itsllplvlf pllgvlstae tcscymndti mvfigglila
      121 vaiehsklhl rialgvmkmv gcshgrllag lcvvttfism wvsntaatam mvpiifailn
      181 eleqvscces ltlhdstynl fllltiqagl grvfnkipad pdnmdqepel qptrvtkayl
      241 maaaycstfg gtgtlvgtgt nltfkgiyen tfpesgginf tqwmfasipq mivntaltwl
      301 ylrimfmgfl rpgskdaeqs nigpegeavt nrvvqqrysd lgnitfhekg vatlfiacif
      361 lwifrkpgfv rgwseaisdi dirdstpvil vsilmffipk dptfiysysp drkcipmkfl
      421 ylvillslsn lylqvrsfrf aaekrpqkas eglitwdvie tkmpwslmfl lgggfaiskg
      481 siasclakri gdalvplkhl ppviilvlvs lfigtiteft snvgianitl pvvaqmcvam
      541 eihplylmvp atimcsysfr lpvgtppnai vtivgriptk wlmiggcapa iyslivqssf
      601 fvvwgvyvyg ahgfpswasn lhasnstskc d
//
LOCUS       XP_076545540             608 aa            linear   INV 03-SEP-2025
DEFINITION  protein I'm not dead yet isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545540
VERSION     XP_076545540.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689425.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..608
                     /product="protein I'm not dead yet isoform X2"
                     /calculated_mol_wt=67487
     Region          <33..183
                     /region_name="ArsB_NhaD_permease"
                     /note="Anion permease ArsB/NhaD. These permeases have been
                     shown to translocate sodium, arsenate, antimonite, sulfate
                     and organic anions across biological membranes in all
                     three kingdoms of life. A typical anion permease contains
                     8-13 transmembrane helices...; cl21473"
                     /db_xref="CDD:473875"
     Region          114..581
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..608
                     /gene="LOC117606426"
                     /coded_by="XM_076689425.1:150..1976"
                     /db_xref="GeneID:117606426"
ORIGIN      
        1 mkqqarsrqe qeggegeeeh nkwwiltlrf lktywkiifa itwamflapf lfiynypevr
       61 caytillmag ywicecfpla itsllplvlf pllgvlstae tcscymndti mvfigglila
      121 vaiehsklhl rialgvmkmv gcshgrllag lcvvttfism wvsntaatam mvpiifailn
      181 eleqaglgrv fnkipadpdn mdqepelqpt rvtkaylmaa aycstfggtg tlvgtgtnlt
      241 fkgiyentfp esgginftqw mfasipqmiv ntaltwlylr imfmgflrpg skdaeqsnig
      301 pegeavtnrv vqqrysdlgn itfhekgvat lfiaciflwi frkpgfvrgw seaisdidir
      361 dstpvilvsi lmffipkdpt fiysyspdrk cipmkflylv illslsnlyl qvrsfrfaae
      421 krpqkasegl itwdvietkm pwslmfllgg gfaiskgsia sclakrigda lvplkhlppv
      481 iilvlvslfi gtiteftsnv gianitlpvv aqmcvameih plylmvpati mcsysfrlpv
      541 gtppnaivti vgriptkwlm iggcapaiys livqssffvv wgvyvygahg fpswasnlha
      601 snstskcd
//
LOCUS       XP_034184723             601 aa            linear   INV 03-SEP-2025
DEFINITION  protein I'm not dead yet isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034184723
VERSION     XP_034184723.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328832.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184723.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..601
                     /product="protein I'm not dead yet isoform X3"
                     /calculated_mol_wt=66494
     Region          <33..183
                     /region_name="ArsB_NhaD_permease"
                     /note="Anion permease ArsB/NhaD. These permeases have been
                     shown to translocate sodium, arsenate, antimonite, sulfate
                     and organic anions across biological membranes in all
                     three kingdoms of life. A typical anion permease contains
                     8-13 transmembrane helices...; cl21473"
                     /db_xref="CDD:473875"
     Region          114..574
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..601
                     /gene="LOC117606426"
                     /coded_by="XM_034328832.2:157..1962"
                     /db_xref="GeneID:117606426"
ORIGIN      
        1 mkqqarsrqe qeggegeeeh nkwwiltlrf lktywkiifa itwamflapf lfiynypevr
       61 caytillmag ywicecfpla itsllplvlf pllgvlstae tcscymndti mvfigglila
      121 vaiehsklhl rialgvmkmv gcshgrllag lcvvttfism wvsntaatam mvpiifailn
      181 eleqvscces ltlhdstynl fllltiqagl grvfnkipad pdnmdqepel qptrvtkayl
      241 maaaycstfg gtgtlvgtgt nltfkgiyen tfpesgginf tqwmfasipq mivntaltwl
      301 ylrimfmgfl rpgskdaeqs nigpegeavt nrvvqqrysd lgnitfhekg vatlfiacif
      361 lwifrkpgfv rgwseaisdi dirdstpvil vsilmffipk dptfiysysp dpekrpqkas
      421 eglitwdvie tkmpwslmfl lgggfaiskg siasclakri gdalvplkhl ppviilvlvs
      481 lfigtiteft snvgianitl pvvaqmcvam eihplylmvp atimcsysfr lpvgtppnai
      541 vtivgriptk wlmiggcapa iyslivqssf fvvwgvyvyg ahgfpswasn lhasnstskc
      601 d
//
LOCUS       XP_034184724             578 aa            linear   INV 03-SEP-2025
DEFINITION  protein I'm not dead yet isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034184724
VERSION     XP_034184724.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328833.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184724.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="protein I'm not dead yet isoform X4"
                     /calculated_mol_wt=63898
     Region          <33..183
                     /region_name="ArsB_NhaD_permease"
                     /note="Anion permease ArsB/NhaD. These permeases have been
                     shown to translocate sodium, arsenate, antimonite, sulfate
                     and organic anions across biological membranes in all
                     three kingdoms of life. A typical anion permease contains
                     8-13 transmembrane helices...; cl21473"
                     /db_xref="CDD:473875"
     Region          114..551
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..578
                     /gene="LOC117606426"
                     /coded_by="XM_034328833.2:154..1890"
                     /db_xref="GeneID:117606426"
ORIGIN      
        1 mkqqarsrqe qeggegeeeh nkwwiltlrf lktywkiifa itwamflapf lfiynypevr
       61 caytillmag ywicecfpla itsllplvlf pllgvlstae tcscymndti mvfigglila
      121 vaiehsklhl rialgvmkmv gcshgrllag lcvvttfism wvsntaatam mvpiifailn
      181 eleqaglgrv fnkipadpdn mdqepelqpt rvtkaylmaa aycstfggtg tlvgtgtnlt
      241 fkgiyentfp esgginftqw mfasipqmiv ntaltwlylr imfmgflrpg skdaeqsnig
      301 pegeavtnrv vqqrysdlgn itfhekgvat lfiaciflwi frkpgfvrgw seaisdidir
      361 dstpvilvsi lmffipkdpt fiysyspdpe krpqkasegl itwdvietkm pwslmfllgg
      421 gfaiskgsia sclakrigda lvplkhlppv iilvlvslfi gtiteftsnv gianitlpvv
      481 aqmcvameih plylmvpati mcsysfrlpv gtppnaivti vgriptkwlm iggcapaiys
      541 livqssffvv wgvyvygahg fpswasnlha snstskcd
//
LOCUS       XP_034184731             573 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/hydrogen exchanger 9B1 [Osmia lignaria lignaria].
ACCESSION   XP_034184731
VERSION     XP_034184731.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328840.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184731.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="sodium/hydrogen exchanger 9B1"
                     /calculated_mol_wt=64005
     Region          125..567
                     /region_name="NhaP"
                     /note="NhaP-type Na+/H+ or K+/H+ antiporter [Inorganic ion
                     transport and metabolism]; COG0025"
                     /db_xref="CDD:439796"
     CDS             1..573
                     /gene="LOC117606429"
                     /coded_by="XM_034328840.2:4652..6373"
                     /db_xref="GeneID:117606429"
ORIGIN      
        1 malwkrdrke esgedseert dssrhskanp dsnfdsdsns nldgdskkle qddmtcchri
       61 tvcspvirel lvtepvsryl grhvtwahff sttsnmalat ivwaflyfll gdtmlptndg
      121 fglyvlvifs sflgrslssi pylnlppvfg mllaglivrn tglfniqeel gvantakirt
      181 fcltfvaira glqlsttslk rnpiflisla iipctmemfg vtlfcryvlf yswnwsfmtg
      241 tilacmspvv tvncvlalae rgygedkgla ailctaacid nvhmislfav cytnlfsndq
      301 eksewwsyip aglrdtllgi gtglclgiff vffphrsyky atsyriiclv saslmcttam
      361 aeisisgggf lasmvlsfia itgwkilsds fdtapfrqat yilwqlvqpi lvgvigadin
      421 fnhwtlsrls lhllciligl svrsltilfa sfrspftwke rlfvivcwlp kgtlqaamap
      481 mayervrhra ngrmielavd vvkvsvltml flapigaili nltgpillnk itkeeqrrkr
      541 elsylrilsl qpfshqsvrk keikrrlsgv vta
//
LOCUS       XP_076545541             573 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/hydrogen exchanger 9B1 [Osmia lignaria lignaria].
ACCESSION   XP_076545541
VERSION     XP_076545541.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689426.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="sodium/hydrogen exchanger 9B1"
                     /calculated_mol_wt=64005
     Region          125..567
                     /region_name="NhaP"
                     /note="NhaP-type Na+/H+ or K+/H+ antiporter [Inorganic ion
                     transport and metabolism]; COG0025"
                     /db_xref="CDD:439796"
     CDS             1..573
                     /gene="LOC117606429"
                     /coded_by="XM_076689426.1:4620..6341"
                     /db_xref="GeneID:117606429"
ORIGIN      
        1 malwkrdrke esgedseert dssrhskanp dsnfdsdsns nldgdskkle qddmtcchri
       61 tvcspvirel lvtepvsryl grhvtwahff sttsnmalat ivwaflyfll gdtmlptndg
      121 fglyvlvifs sflgrslssi pylnlppvfg mllaglivrn tglfniqeel gvantakirt
      181 fcltfvaira glqlsttslk rnpiflisla iipctmemfg vtlfcryvlf yswnwsfmtg
      241 tilacmspvv tvncvlalae rgygedkgla ailctaacid nvhmislfav cytnlfsndq
      301 eksewwsyip aglrdtllgi gtglclgiff vffphrsyky atsyriiclv saslmcttam
      361 aeisisgggf lasmvlsfia itgwkilsds fdtapfrqat yilwqlvqpi lvgvigadin
      421 fnhwtlsrls lhllciligl svrsltilfa sfrspftwke rlfvivcwlp kgtlqaamap
      481 mayervrhra ngrmielavd vvkvsvltml flapigaili nltgpillnk itkeeqrrkr
      541 elsylrilsl qpfshqsvrk keikrrlsgv vta
//
LOCUS       XP_034184733             547 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein CG3556 [Osmia lignaria lignaria].
ACCESSION   XP_034184733
VERSION     XP_034184733.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328842.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184733.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..547
                     /product="uncharacterized protein CG3556"
                     /calculated_mol_wt=59245
     Region          74..269
                     /region_name="Cobalamin_bind"
                     /note="Eukaryotic cobalamin-binding protein; pfam01122"
                     /db_xref="CDD:460073"
     CDS             1..547
                     /gene="LOC117606430"
                     /coded_by="XM_034328842.2:370..2013"
                     /db_xref="GeneID:117606430"
ORIGIN      
        1 mcisilrswm tmwgrasmag vmvlmlayvv rgeggigeig stdteadkil eratawlwsq
       61 rdkdagwgnd tnrvllvlrl snlsrednva piaslelqls skqmeleivl llwrhrevgf
      121 spvrlarytl alnamctdpr qfhghdligt lqhhepptdy efalttlaac naqahvrkrq
      181 irrlldiana aqdhnvdtva mvilalkciv qdhrhrnlhh fvrkpsigla qqqrldgsfg
      241 dvrttalvmq aleeaenepa dnwnrsaals wltsqqradg sfagdvrvta eallgvaprg
      301 lasirtldcg qglaetsppr ftiaanepvi snnrnesvat lliagnnast vatgtgnngt
      361 ngnarnsgns innvnnvnsg nnvrntlnsn ntendainaa aagaaaataa anatatataa
      421 aaaavaasvp vmvnvsytlw vgsnvnetys lmvtapknet fyevmvlaae msphfqfvss
      481 kwpnghyvha iagykeepms yhywllyrlt alpdpssppg nqlvapggvd dlqisdgehy
      541 lfwykkl
//
LOCUS       XP_034184735             547 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein CG3556 [Osmia lignaria lignaria].
ACCESSION   XP_034184735
VERSION     XP_034184735.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328844.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184735.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..547
                     /product="uncharacterized protein CG3556"
                     /calculated_mol_wt=59245
     Region          74..269
                     /region_name="Cobalamin_bind"
                     /note="Eukaryotic cobalamin-binding protein; pfam01122"
                     /db_xref="CDD:460073"
     CDS             1..547
                     /gene="LOC117606430"
                     /coded_by="XM_034328844.2:50..1693"
                     /db_xref="GeneID:117606430"
ORIGIN      
        1 mcisilrswm tmwgrasmag vmvlmlayvv rgeggigeig stdteadkil eratawlwsq
       61 rdkdagwgnd tnrvllvlrl snlsrednva piaslelqls skqmeleivl llwrhrevgf
      121 spvrlarytl alnamctdpr qfhghdligt lqhhepptdy efalttlaac naqahvrkrq
      181 irrlldiana aqdhnvdtva mvilalkciv qdhrhrnlhh fvrkpsigla qqqrldgsfg
      241 dvrttalvmq aleeaenepa dnwnrsaals wltsqqradg sfagdvrvta eallgvaprg
      301 lasirtldcg qglaetsppr ftiaanepvi snnrnesvat lliagnnast vatgtgnngt
      361 ngnarnsgns innvnnvnsg nnvrntlnsn ntendainaa aagaaaataa anatatataa
      421 aaaavaasvp vmvnvsytlw vgsnvnetys lmvtapknet fyevmvlaae msphfqfvss
      481 kwpnghyvha iagykeepms yhywllyrlt alpdpssppg nqlvapggvd dlqisdgehy
      541 lfwykkl
//
LOCUS       XP_076545543             538 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606432 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545543
VERSION     XP_076545543.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689428.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..538
                     /product="uncharacterized protein LOC117606432 isoform X1"
                     /calculated_mol_wt=62614
     Region          <43..>195
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..538
                     /gene="LOC117606432"
                     /coded_by="XM_076689428.1:231..1847"
                     /db_xref="GeneID:117606432"
ORIGIN      
        1 mvdlfekdkg nislsaklqs wqdekekskp tiintqkafy sikigdiqnk inrledrnde
       61 llekievtde rsaiidaett deianlskql ysqnnkvddv ktkidnvenn rikdkhshdd
      121 kvellnqryk ekkvelvsri kvltakingl edfkktqrgl eekferneee isknekemke
      181 llesvnrkfe fdkemlknem ylclldlaaq fqvetvehis lpnkrlmren imlknelshi
      241 skqlssendi qsilknsvvd yrknivkksl hvkeniitik iqnallkclk nkfkstkgyf
      301 ssidvpdram eckylmlien arneenltnv rlsklnvlle kertrigiak ylhrrlncki
      361 kaavdtlydl kyiarcllec ptkcpdiatl nctqmllflq nifmkgqlkv qcgivksvet
      421 lpqvlefrte dlnnisdfei itesqekeqi ldkvrknnfi tslkhvlcfi leisspyqvk
      481 lssdsdketk isiesidlke hsstdisdni mpfelkddyk lsesdeyied esvfsive
//
LOCUS       XP_034184743             513 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606432 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184743
VERSION     XP_034184743.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328852.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184743.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..513
                     /product="uncharacterized protein LOC117606432 isoform X2"
                     /calculated_mol_wt=59682
     Region          <43..>195
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..513
                     /gene="LOC117606432"
                     /coded_by="XM_034328852.2:230..1771"
                     /db_xref="GeneID:117606432"
ORIGIN      
        1 mvdlfekdkg nislsaklqs wqdekekskp tiintqkafy sikigdiqnk inrledrnde
       61 llekievtde rsaiidaett deianlskql ysqnnkvddv ktkidnvenn rikdkhshdd
      121 kvellnqryk ekkvelvsri kvltakingl edfkktqrgl eekferneee isknekemke
      181 llesvnrkfe fdkemlknem ylclldlaaq fqvetvehis lpnkrlmren imlknelshi
      241 skqlssendi qsilknsvvd yrknivkksl hvkeniitik iqnallkclk nkfkstkgyf
      301 ssidvpdram eckylmlien arneenltnv rlsklnvlle kertrigiak ylhrrlncki
      361 kaavdtlydl kyiarcllec ptkcpdiatl nctqmllflq nifmkgqlkv qcgivksvet
      421 lpqvlefrte dlnnisdfei itesqekeqi ldkvklssds dketkisies idlkehsstd
      481 isdnimpfel kddyklsesd eyiedesvfs ive
//
LOCUS       XP_034184747             512 aa            linear   INV 03-SEP-2025
DEFINITION  gustatory receptor for sugar taste 43a isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184747
VERSION     XP_034184747.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328856.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184747.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="gustatory receptor for sugar taste 43a isoform
                     X1"
                     /calculated_mol_wt=57832
     Region          66..477
                     /region_name="7tm_7"
                     /note="7tm Chemosensory receptor; pfam08395"
                     /db_xref="CDD:462463"
     CDS             1..512
                     /gene="LOC117606434"
                     /coded_by="XM_034328856.2:4..1542"
                     /db_xref="GeneID:117606434"
ORIGIN      
        1 masrirwnvd nrskrstngn gngngngngn gngngngnds itndrmeaei kkdtnpssks
       61 dlcraispiy ylgkvcglvp vrfvanttgg fqarpnifdl vyslcvltll lgaeiwglwr
      121 dlkdgwvhst rlkfrtavia tcsdvlgvms ltvvcivgsp frwkhlqvvv nklievdkkv
      181 dlsstkknar rftislttcs llylwfnstl dfyswyrktk vhqtmsdkgp inyaplyfmy
      241 tviisteiqy tvsiyniger fvrlnnslrk lfdtdtltny lrkfpeideh vskktstdlp
      301 qrpfelasyr vfpelhenks yvnsiselrw ihsslcdtvs linntfgavi ltvtvtcllh
      361 liitpyfliv qagekhewif lfvqvgwcif hvtrmlmivq psystvaegk ktailvsqll
      421 sstfetdtrr eleifslqll hrplefsacg lfsldrtlit siagvvttyl viliqfqnad
      481 dtkgdadiir nvtqifknvs plqnftgiki ia
//
LOCUS       XP_034184748             483 aa            linear   INV 03-SEP-2025
DEFINITION  gustatory receptor for sugar taste 43a isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184748
VERSION     XP_034184748.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328857.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184748.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="gustatory receptor for sugar taste 43a isoform
                     X2"
                     /calculated_mol_wt=54422
     Region          66..448
                     /region_name="7tm_7"
                     /note="7tm Chemosensory receptor; pfam08395"
                     /db_xref="CDD:462463"
     CDS             1..483
                     /gene="LOC117606434"
                     /coded_by="XM_034328857.2:4..1455"
                     /db_xref="GeneID:117606434"
ORIGIN      
        1 masrirwnvd nrskrstngn gngngngngn gngngngnds itndrmeaei kkdtnpssks
       61 dlcraispiy ylgkvcglvp vrfvanttgg fqarpnifdl vyslcvltll lgaeiwglwr
      121 dlkdgwvhst rlkfrtavia tcsdvlgvms ltvvcivgsp frwkhlqvvv nklievdkkv
      181 dlsstkknar rftislttcs llylwfnstl dfyswyrktk vhqtmsdkgp inyaplyfmy
      241 tviisteiqy tvsiyniger fvrlnnslrk lfdtdtltny lrkfpeienk syvnsiselr
      301 wihsslcdtv slinntfgav iltvtvtcll hliitpyfli vqagekhewi flfvqvgwci
      361 fhvtrmlmiv qpsystvaeg kktailvsql lsstfetdtr releifslql lhrplefsac
      421 glfsldrtli tsiagvvtty lviliqfqna ddtkgdadii rnvtqifknv splqnftgik
      481 iia
//
LOCUS       XP_076545544             424 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606434 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545544
VERSION     XP_076545544.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689429.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..424
                     /product="uncharacterized protein LOC117606434 isoform X3"
                     /calculated_mol_wt=47413
     Region          66..389
                     /region_name="7tm_7"
                     /note="7tm Chemosensory receptor; pfam08395"
                     /db_xref="CDD:462463"
     CDS             1..424
                     /gene="LOC117606434"
                     /coded_by="XM_076689429.1:4..1278"
                     /db_xref="GeneID:117606434"
ORIGIN      
        1 masrirwnvd nrskrstngn gngngngngn gngngngnds itndrmeaei kkdtnpssks
       61 dlcraispiy ylgkvcglvp vrfvanttgg fqarpnifdl vyslcvltll lgaeiwglwr
      121 dlkdgwvhst rlkfrtavia tcsdvlgvms ltvvcivgsp frwkhlqvvv nklievdkkv
      181 dlsstkknar rftislttcs llylwfnstl dfyswyrktk vhqtmsdken ksyvnsisel
      241 rwihsslcdt vslinntfga viltvtvtcl lhliitpyfl ivqagekhew iflfvqvgwc
      301 ifhvtrmlmi vqpsystvae gkktailvsq llsstfetdt rreleifslq llhrplefsa
      361 cglfsldrtl itsiagvvtt ylviliqfqn addtkgdadi irnvtqifkn vsplqnftgi
      421 kiia
//
LOCUS       XP_076545545             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305500 [Osmia lignaria lignaria].
ACCESSION   XP_076545545
VERSION     XP_076545545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689430.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC143305500"
                     /calculated_mol_wt=54914
     CDS             1..479
                     /gene="LOC143305500"
                     /coded_by="XM_076689430.1:132..1571"
                     /db_xref="GeneID:143305500"
ORIGIN      
        1 mfsfqvqkhc trllrkmtlv ssnneskngk dppqqnheet mqtstpsgls elqmqstevg
       61 eitempdfem lstsavlhyc khkilrpylr llgvmglkpm ssdddsercs cyciltnlht
      121 lqviifmcig yilqymacfr rdrgfcyktl pleleivani skehteeiic fgniifsylv
      181 psvlhlvayl ytvylfrike neqlqnlmer afllssnpvn rgnqrklvri lwlfialsvv
      241 wimmalitvn ilmaqgsivf qwleqspyqv kitlkvfliv ctlwhdmvqg tiitsyclqg
      301 qlllahlyfl rgkllhhtls pidwmkeice fkkllkyfnd elgpavciyt vvnlswaaag
      361 tiwllrydny ngdaqnspit wigivnvvlw ilisivpfiq aarlstacsm iqsighevri
      421 rpfvyqstpg edldtillyt sslkmcarlf rvpvtgrylc llltvgsili ltlgqcqff
//
LOCUS       XP_034184759             445 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome biogenesis protein NOP53 [Osmia lignaria lignaria].
ACCESSION   XP_034184759
VERSION     XP_034184759.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328868.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="ribosome biogenesis protein NOP53"
                     /calculated_mol_wt=52271
     Region          18..404
                     /region_name="Nop53"
                     /note="Nop53 (60S ribosomal biogenesis); pfam07767"
                     /db_xref="CDD:462259"
     CDS             1..445
                     /gene="LOC117606438"
                     /coded_by="XM_034328868.2:126..1463"
                     /db_xref="GeneID:117606438"
ORIGIN      
        1 mpainskkrk vskktkrswr khvdtkdvek fledsrleer lggpfstrqd selfivstep
       61 evlpkrtrre ilkskeprcf nilkphtavq dpirkrnrvr tkeerknail vekeafreaq
      121 gilklkdkir lqnrmlamke rsnkpkrgef nadvwegkfe eqlgidtewm spdtvrhtlt
      181 hfgakkrrip isvrkkpsal paieaphpgt synpsfedhq tllhqiakke melikeeqhl
      241 krvttdmfkk vsveekennl lkemleglks eekndpdedl dptvtsvnrp vrnekktrvq
      301 rrkqkeekel arkkqqkkle kkkvsdiykl rllnkqlask erkekilree rlkkkalkav
      361 gpktlskvkf epleadfkla nelsgnlrna epttnllkdr fkslqqrniv apsrmklkrd
      421 kakvkrfvkp dhkidvtiyq temtk
//
LOCUS       XP_034184801             196 aa            linear   INV 03-SEP-2025
DEFINITION  protein OPI10 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034184801
VERSION     XP_034184801.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328910.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="protein OPI10 homolog"
                     /calculated_mol_wt=21574
     Region          1..194
                     /region_name="DUF775"
                     /note="Protein of unknown function (DUF775); pfam05603"
                     /db_xref="CDD:461690"
     CDS             1..196
                     /gene="LOC117606460"
                     /coded_by="XM_034328910.2:150..740"
                     /db_xref="GeneID:117606460"
ORIGIN      
        1 mlgiivsgrl vqtdfqqige nqflitvpda dninhivvfl tgtipfpdgt ggavyfswpd
       61 ptappnwqfl gyisnvkpsa ifkistlkkn hefensnlgi fgvgkishva qigvsveplg
      121 aieqlaasvt eatsnsflef vqkmltsfln yvtsfsvtqa qmapnpneny vplstiqgwy
      181 etferrlqqn pnfwka
//
LOCUS       XP_034184810             149 aa            linear   INV 03-SEP-2025
DEFINITION  PTIP binding protein Pa1 [Osmia lignaria lignaria].
ACCESSION   XP_034184810
VERSION     XP_034184810.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184810.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="PTIP binding protein Pa1"
                     /calculated_mol_wt=17129
     Region          7..132
                     /region_name="PAXIP1_C"
                     /note="PAXIP1-associated-protein-1 C term PTIP binding
                     protein; pfam15364"
                     /db_xref="CDD:464674"
     CDS             1..149
                     /gene="Pa1"
                     /coded_by="XM_034328919.2:691..1140"
                     /db_xref="GeneID:117606463"
ORIGIN      
        1 merneedwsv ecsddekyem drknewtlks ddiltlieal etnnrilele wkcpgrrgps
       61 pvlsnnrkqd ngsqeykiee ksdfdfmdem ssprlpvrri gestpkgsak kktasfngvl
      121 stmlrhrrle qqeinsspkk sesssvskt
//
LOCUS       XP_034184809             149 aa            linear   INV 03-SEP-2025
DEFINITION  PTIP binding protein Pa1 [Osmia lignaria lignaria].
ACCESSION   XP_034184809
VERSION     XP_034184809.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328918.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184809.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="PTIP binding protein Pa1"
                     /calculated_mol_wt=17129
     Region          7..132
                     /region_name="PAXIP1_C"
                     /note="PAXIP1-associated-protein-1 C term PTIP binding
                     protein; pfam15364"
                     /db_xref="CDD:464674"
     CDS             1..149
                     /gene="Pa1"
                     /coded_by="XM_034328918.2:691..1140"
                     /db_xref="GeneID:117606463"
ORIGIN      
        1 merneedwsv ecsddekyem drknewtlks ddiltlieal etnnrilele wkcpgrrgps
       61 pvlsnnrkqd ngsqeykiee ksdfdfmdem ssprlpvrri gestpkgsak kktasfngvl
      121 stmlrhrrle qqeinsspkk sesssvskt
//
LOCUS       XP_076545546             149 aa            linear   INV 03-SEP-2025
DEFINITION  PTIP binding protein Pa1 [Osmia lignaria lignaria].
ACCESSION   XP_076545546
VERSION     XP_076545546.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689431.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="PTIP binding protein Pa1"
                     /calculated_mol_wt=17129
     Region          7..132
                     /region_name="PAXIP1_C"
                     /note="PAXIP1-associated-protein-1 C term PTIP binding
                     protein; pfam15364"
                     /db_xref="CDD:464674"
     CDS             1..149
                     /gene="Pa1"
                     /coded_by="XM_076689431.1:691..1140"
                     /db_xref="GeneID:117606463"
ORIGIN      
        1 merneedwsv ecsddekyem drknewtlks ddiltlieal etnnrilele wkcpgrrgps
       61 pvlsnnrkqd ngsqeykiee ksdfdfmdem ssprlpvrri gestpkgsak kktasfngvl
      121 stmlrhrrle qqeinsspkk sesssvskt
//
LOCUS       XP_076545547             149 aa            linear   INV 03-SEP-2025
DEFINITION  PTIP binding protein Pa1 [Osmia lignaria lignaria].
ACCESSION   XP_076545547
VERSION     XP_076545547.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689432.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="PTIP binding protein Pa1"
                     /calculated_mol_wt=17129
     Region          7..132
                     /region_name="PAXIP1_C"
                     /note="PAXIP1-associated-protein-1 C term PTIP binding
                     protein; pfam15364"
                     /db_xref="CDD:464674"
     CDS             1..149
                     /gene="Pa1"
                     /coded_by="XM_076689432.1:691..1140"
                     /db_xref="GeneID:117606463"
ORIGIN      
        1 merneedwsv ecsddekyem drknewtlks ddiltlieal etnnrilele wkcpgrrgps
       61 pvlsnnrkqd ngsqeykiee ksdfdfmdem ssprlpvrri gestpkgsak kktasfngvl
      121 stmlrhrrle qqeinsspkk sesssvskt
//
LOCUS       XP_034184813             126 aa            linear   INV 03-SEP-2025
DEFINITION  profilin chickadee [Osmia lignaria lignaria].
ACCESSION   XP_034184813
VERSION     XP_034184813.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328922.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..126
                     /product="profilin chickadee"
                     /calculated_mol_wt=13599
     Region          1..121
                     /region_name="Profilin"
                     /note="pfam00235"
                     /db_xref="CDD:459724"
     CDS             1..126
                     /gene="chic"
                     /coded_by="XM_034328922.2:123..503"
                     /db_xref="GeneID:117606465"
ORIGIN      
        1 mswqdyvdkq llasrcvtka aigghdgnvw aksegfevsk eelaklvqgf ddqdiltssg
       61 vtlagnryiy lsgtdrvira klgkvgvhcm kttqsvvvsl yedpiqpqqa asvveklgdy
      121 llacgy
//
LOCUS       XP_034190412             628 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase AMFR isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190412
VERSION     XP_034190412.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334521.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190412.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="E3 ubiquitin-protein ligase AMFR isoform X1"
                     /calculated_mol_wt=72705
     Region          98..>421
                     /region_name="HRD1"
                     /note="HRD ubiquitin ligase complex, ER membrane component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5243"
                     /db_xref="CDD:227568"
     Region          473..513
                     /region_name="CUE_AMFR"
                     /note="CUE domain found in autocrine motility factor
                     receptor (AMFR) and similar proteins; cd14421"
                     /db_xref="CDD:270604"
     CDS             1..628
                     /gene="LOC117608849"
                     /coded_by="XM_034334521.2:215..2101"
                     /db_xref="GeneID:117608849"
ORIGIN      
        1 mqtrkhiirg srnkakakmp mefldrlpip nlrvytaisf avlscsvyya aeiikdpawk
       61 snhtyvdtet esptgdvesd prnfaihfke llecmldepi ciwtlinmay cvlillgkti
      121 qklvfgelra serqhlkdkf wnfvfykfif vfgvlnvqyl devvlwwawf tvlgflslls
      181 qlckdrfeyl sfspttpgws harllgllat ilalssfmll lctaaafffv sfntfvftaa
      241 ecillgvrtv hvmvryiihl ydtrgagtss qrswdkrgpl tyytdlaael ivlaidflhh
      301 ihmllwsnif lsmaslvicm qlrylfyeiq rkitkhrnyl avlnhmeqny pmatqeelad
      361 nsdncaicwe kmetarklpc ghlfhnsclq swleqdtscp tcrlalnmqp nhrentqelp
      421 nepqtparrn enhffhfdgs ryvswlpsfs vevshnrlrg nistiahnns qmdtmirqvq
      481 qlfphfprnl ivedlrmtrs vewtvenild gvlisphhli eepqsesvpq ihtniteisa
      541 slntastplp sdprfdipla dssrvicvnd ysgeepsnig grfskssser eqilqrrkah
      601 mrlaarrryf ekrrkkheve qrisdtts
//
LOCUS       XP_034190413             627 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase AMFR isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190413
VERSION     XP_034190413.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334522.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190413.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="E3 ubiquitin-protein ligase AMFR isoform X2"
                     /calculated_mol_wt=72618
     Region          98..>421
                     /region_name="HRD1"
                     /note="HRD ubiquitin ligase complex, ER membrane component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5243"
                     /db_xref="CDD:227568"
     Region          473..513
                     /region_name="CUE_AMFR"
                     /note="CUE domain found in autocrine motility factor
                     receptor (AMFR) and similar proteins; cd14421"
                     /db_xref="CDD:270604"
     CDS             1..627
                     /gene="LOC117608849"
                     /coded_by="XM_034334522.2:215..2098"
                     /db_xref="GeneID:117608849"
ORIGIN      
        1 mqtrkhiirg srnkakakmp mefldrlpip nlrvytaisf avlscsvyya aeiikdpawk
       61 snhtyvdtet esptgdvesd prnfaihfke llecmldepi ciwtlinmay cvlillgkti
      121 qklvfgelra serqhlkdkf wnfvfykfif vfgvlnvqyl devvlwwawf tvlgflslls
      181 qlckdrfeyl sfspttpgws harllgllat ilalssfmll lctaaafffv sfntfvftaa
      241 ecillgvrtv hvmvryiihl ydtrgagtss qrswdkrgpl tyytdlaael ivlaidflhh
      301 ihmllwsnif lsmaslvicm qlrylfyeiq rkitkhrnyl avlnhmeqny pmatqeelad
      361 nsdncaicwe kmetarklpc ghlfhnsclq swleqdtscp tcrlalnmqp nhrentqelp
      421 nepqtparrn enhffhfdgs ryvswlpsfs vevshnrlrg nistiahnns qmdtmirqvq
      481 qlfphfprnl ivedlrmtrs vewtvenild gvlisphhli eepqsesvpq ihtniteisa
      541 slntastplp sdprfdipla dsrvicvndy sgeepsnigg rfsksssere qilqrrkahm
      601 rlaarrryfe krrkkheveq risdtts
//
LOCUS       XP_034190414             618 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase AMFR isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190414
VERSION     XP_034190414.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334523.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190414.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..618
                     /product="E3 ubiquitin-protein ligase AMFR isoform X3"
                     /calculated_mol_wt=71568
     Region          98..>421
                     /region_name="HRD1"
                     /note="HRD ubiquitin ligase complex, ER membrane component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5243"
                     /db_xref="CDD:227568"
     Region          473..513
                     /region_name="CUE_AMFR"
                     /note="CUE domain found in autocrine motility factor
                     receptor (AMFR) and similar proteins; cd14421"
                     /db_xref="CDD:270604"
     CDS             1..618
                     /gene="LOC117608849"
                     /coded_by="XM_034334523.2:126..1982"
                     /db_xref="GeneID:117608849"
ORIGIN      
        1 mqtrkhiirg srnkakakmp mefldrlpip nlrvytaisf avlscsvyya aeiikdpawk
       61 snhtyvdtet esptgdvesd prnfaihfke llecmldepi ciwtlinmay cvlillgkti
      121 qklvfgelra serqhlkdkf wnfvfykfif vfgvlnvqyl devvlwwawf tvlgflslls
      181 qlckdrfeyl sfspttpgws harllgllat ilalssfmll lctaaafffv sfntfvftaa
      241 ecillgvrtv hvmvryiihl ydtrgagtss qrswdkrgpl tyytdlaael ivlaidflhh
      301 ihmllwsnif lsmaslvicm qlrylfyeiq rkitkhrnyl avlnhmeqny pmatqeelad
      361 nsdncaicwe kmetarklpc ghlfhnsclq swleqdtscp tcrlalnmqp nhrentqelp
      421 nepqtparrn enhffhfdgs ryvswlpsfs vevshnrlrg nistiahnns qmdtmirqvq
      481 qlfphfprnl ivedlrmtrs vewtvenild gvlisphhli eepqsesvpq ihtniteisa
      541 slntastplp sdprfdipla dsgeepsnig grfskssser eqilqrrkah mrlaarrryf
      601 ekrrkkheve qrisdtts
//
LOCUS       XP_034190415             610 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase AMFR isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190415
VERSION     XP_034190415.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334524.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190415.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="E3 ubiquitin-protein ligase AMFR isoform X4"
                     /calculated_mol_wt=70599
     Region          80..>403
                     /region_name="HRD1"
                     /note="HRD ubiquitin ligase complex, ER membrane component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5243"
                     /db_xref="CDD:227568"
     Region          455..495
                     /region_name="CUE_AMFR"
                     /note="CUE domain found in autocrine motility factor
                     receptor (AMFR) and similar proteins; cd14421"
                     /db_xref="CDD:270604"
     CDS             1..610
                     /gene="LOC117608849"
                     /coded_by="XM_034334524.2:470..2302"
                     /db_xref="GeneID:117608849"
ORIGIN      
        1 mpmefldrlp ipnlrvytai sfavlscsvy yaaeiikdpa wksnhtyvdt etesptgdve
       61 sdprnfaihf kellecmlde piciwtlinm aycvlillgk tiqklvfgel raserqhlkd
      121 kfwnfvfykf ifvfgvlnvq yldevvlwwa wftvlgflsl lsqlckdrfe ylsfspttpg
      181 wsharllgll atilalssfm lllctaaaff fvsfntfvft aaecillgvr tvhvmvryii
      241 hlydtrgagt ssqrswdkrg pltyytdlaa elivlaidfl hhihmllwsn iflsmaslvi
      301 cmqlrylfye iqrkitkhrn ylavlnhmeq nypmatqeel adnsdncaic wekmetarkl
      361 pcghlfhnsc lqswleqdts cptcrlalnm qpnhrentqe lpnepqtpar rnenhffhfd
      421 gsryvswlps fsvevshnrl rgnistiahn nsqmdtmirq vqqlfphfpr nlivedlrmt
      481 rsvewtveni ldgvlisphh lieepqsesv pqihtnitei saslntastp lpsdprfdip
      541 ladssrvicv ndysgeepsn iggrfsksss ereqilqrrk ahmrlaarrr yfekrrkkhe
      601 veqrisdtts
//
LOCUS       XP_034190499             111 aa            linear   INV 03-SEP-2025
DEFINITION  dolichyl-diphosphooligosaccharide--protein glycosyltransferase
            subunit [Osmia lignaria lignaria].
ACCESSION   XP_034190499
VERSION     XP_034190499.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334608.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..111
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit"
                     /calculated_mol_wt=12401
     Region          6..111
                     /region_name="DAD"
                     /note="DAD family; pfam02109"
                     /db_xref="CDD:460448"
     CDS             1..111
                     /gene="Dad1"
                     /coded_by="XM_034334608.2:90..425"
                     /db_xref="GeneID:117608880"
ORIGIN      
        1 mtaltvigkf lqeytkstpk klkiidayll yvfltgviqf iycclvgtfp fnsflsgfis
       61 cvscfvlgvc lrlqvnpqnk nqfygisper gfadfifahv ilhlvvmnfi g
//
LOCUS       XP_076545549             467 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606473 [Osmia lignaria lignaria].
ACCESSION   XP_076545549
VERSION     XP_076545549.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689434.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="uncharacterized protein LOC117606473"
                     /calculated_mol_wt=52700
     Region          <51..>220
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..467
                     /gene="LOC117606473"
                     /coded_by="XM_076689434.1:298..1701"
                     /db_xref="GeneID:117606473"
ORIGIN      
        1 msgsrlgrgr ggrlavyggc avvvillvfl yraatsemar lrelhvqcah qqealaaqlq
       61 vifeykvrle kslaeekssn aavkqelqqr asrekslrdk dsieamqrfn slqqtykllq
      121 tehqdlqdec kkrekqaled tnrleatlre lrsrirqare dkekalehlk tkfleldtek
      181 trleekyndv lknsgntdst idhlrkevmq lkreleeakk sskarilsps vespqsmqkt
      241 dqqksgspgn leqpvgpsqq qqstattssm kpipamklet stpassgnpv staldkphpl
      301 akpaskaklp vgvppipvmi dqklenrdek rndqevarkk keepkkienp eskeheveng
      361 nleppfpvvs grrgeelpae rrgnrwfnvg pgvqeigdel nhirlpgldd gaernaeagd
      421 dqyegvdydk epqqknsdlq lvegedeged eedqvdyphn lkqekre
//
LOCUS       XP_076545550             467 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606473 [Osmia lignaria lignaria].
ACCESSION   XP_076545550
VERSION     XP_076545550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689435.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="uncharacterized protein LOC117606473"
                     /calculated_mol_wt=52700
     Region          <51..>220
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..467
                     /gene="LOC117606473"
                     /coded_by="XM_076689435.1:162..1565"
                     /db_xref="GeneID:117606473"
ORIGIN      
        1 msgsrlgrgr ggrlavyggc avvvillvfl yraatsemar lrelhvqcah qqealaaqlq
       61 vifeykvrle kslaeekssn aavkqelqqr asrekslrdk dsieamqrfn slqqtykllq
      121 tehqdlqdec kkrekqaled tnrleatlre lrsrirqare dkekalehlk tkfleldtek
      181 trleekyndv lknsgntdst idhlrkevmq lkreleeakk sskarilsps vespqsmqkt
      241 dqqksgspgn leqpvgpsqq qqstattssm kpipamklet stpassgnpv staldkphpl
      301 akpaskaklp vgvppipvmi dqklenrdek rndqevarkk keepkkienp eskeheveng
      361 nleppfpvvs grrgeelpae rrgnrwfnvg pgvqeigdel nhirlpgldd gaernaeagd
      421 dqyegvdydk epqqknsdlq lvegedeged eedqvdyphn lkqekre
//
LOCUS       XP_034185467             424 aa            linear   INV 03-SEP-2025
DEFINITION  traB domain-containing protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185467
VERSION     XP_034185467.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329576.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185467.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..424
                     /product="traB domain-containing protein isoform X1"
                     /calculated_mol_wt=48168
     Region          130..355
                     /region_name="TraB_PrgY-like"
                     /note="TraB/PryY confer plasmid-borne pheromone
                     resistance; cd14726"
                     /db_xref="CDD:350608"
     CDS             1..424
                     /gene="LOC117606734"
                     /coded_by="XM_034329576.2:226..1500"
                     /db_xref="GeneID:117606734"
ORIGIN      
        1 mtskdansge ninaetdkhy cvihkkdvnd qkyshaqkes eklifnelqi dveqnsnsen
       61 esqnetstmd kqeeqstevv gyqlkfdahn qnnktsnesd apsqneydes iderlpetvt
      121 llttpdggkl ylvgtahfsv qsqndvamii ravqphivvv elckaridfl niaeevlyrn
      181 atdlslkslt dmlkefglyg flhfilhrmv thvvkelgmp pggefrtafe eakkvpncii
      241 qladrsisit vqravralsw weiikllwlv rhldshineq dverykrkrv ieemitklke
      301 eypaieqtfv kerdiyltyh lqaatmikyt peglkpariv gvvgighvng ivenwgkvks
      361 sdiwpimrvp pqslssrilk ftfkasligv avyvgykiip lpygsalqsi rssieglfkv
      421 sdrt
//
LOCUS       XP_034185469             423 aa            linear   INV 03-SEP-2025
DEFINITION  traB domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185469
VERSION     XP_034185469.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329578.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185469.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..423
                     /product="traB domain-containing protein isoform X2"
                     /calculated_mol_wt=48039
     Region          129..354
                     /region_name="TraB_PrgY-like"
                     /note="TraB/PryY confer plasmid-borne pheromone
                     resistance; cd14726"
                     /db_xref="CDD:350608"
     CDS             1..423
                     /gene="LOC117606734"
                     /coded_by="XM_034329578.2:227..1498"
                     /db_xref="GeneID:117606734"
ORIGIN      
        1 mtskdansge ninaetdkhy cvihkkdvnd qkyshaqkes eklifnelqi dveqnsnsen
       61 esqnetstmd kqeeqstvvg yqlkfdahnq nnktsnesda psqneydesi derlpetvtl
      121 lttpdggkly lvgtahfsvq sqndvamiir avqphivvve lckaridfln iaeevlyrna
      181 tdlslksltd mlkefglygf lhfilhrmvt hvvkelgmpp ggefrtafee akkvpnciiq
      241 ladrsisitv qravralsww eiikllwlvr hldshineqd verykrkrvi eemitklkee
      301 ypaieqtfvk erdiyltyhl qaatmikytp eglkparivg vvgighvngi venwgkvkss
      361 diwpimrvpp qslssrilkf tfkasligva vyvgykiipl pygsalqsir ssieglfkvs
      421 drt
//
LOCUS       XP_034185470             273 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome c heme lyase [Osmia lignaria lignaria].
ACCESSION   XP_034185470
VERSION     XP_034185470.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329579.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185470.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="cytochrome c heme lyase"
                     /calculated_mol_wt=32022
     Region          43..257
                     /region_name="Cyto_heme_lyase"
                     /note="Cytochrome c/c1 heme lyase; pfam01265"
                     /db_xref="CDD:426167"
     CDS             1..273
                     /gene="Cchl"
                     /coded_by="XM_034329579.2:1..822"
                     /db_xref="GeneID:117606735"
ORIGIN      
        1 mgnvmnfavs atdviipqqr qpqlskvhhe khkpegeppp ecpmhksaek keppkhisec
       61 pihdmnkddi nplnmmpaan qhpapdqpfp lptdrqvssi pkatektefw vypsqqmfwn
      121 amlrkgwrwk nddispkdmd diikihnann eqawlevlkw ealharecst pklrsfggka
      181 tqysprarir ywmgyelpfd rhdwivdrcg knvryiidyy dggqvdekyt falldvrpam
      241 dsleniwdrm rvawmrwrys neskeseyat ksq
//
LOCUS       XP_034185471             251 aa            linear   INV 03-SEP-2025
DEFINITION  putative histone-lysine N-methyltransferase set-23 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185471
VERSION     XP_034185471.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329580.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..251
                     /product="putative histone-lysine N-methyltransferase
                     set-23"
                     /calculated_mol_wt=27320
     Region          15..251
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(109..110,176..181,189..191,217..220)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(109..110,177..181,219)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..251
                     /gene="LOC117606736"
                     /coded_by="XM_034329580.2:1..756"
                     /db_xref="GeneID:117606736"
ORIGIN      
        1 measacpddy ehpipgvmyi vnnipgpgid iedfeseysv gcsctiqctd csctrgfpny
       61 ingriidekl sgpilecnsc ctcgqdcgnr lvqngplnsl kvleasnkgl glftaklikr
      121 gqficeyage vigidearhr feanknsmny vlvvsehigd qnivtcidpk yfgnigryan
      181 hscepntniv piriegttpr lclfacrdie agdeitfsya dgiansvrnf setrclcgss
      241 ncvgylphns i
//
LOCUS       XP_034190264             499 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608780 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190264
VERSION     XP_034190264.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334373.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..499
                     /product="uncharacterized protein LOC117608780 isoform X1"
                     /calculated_mol_wt=53604
     Region          117..>376
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          180..268
                     /region_name="FH_FOXL2"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L2 (FOXL2) and similar proteins; cd20028"
                     /db_xref="CDD:410802"
     Site            order(185,203..204,207,226..227,229..230,233,240,250..252,
                     254)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410802"
     CDS             1..499
                     /gene="LOC117608780"
                     /coded_by="XM_034334373.2:510..2009"
                     /db_xref="GeneID:117608780"
ORIGIN      
        1 mnyadldrtr mvshvipvag elqqgngslg vtglggmqes shslkikqep fqvsppsplp
       61 slhqvssfvs dmqngclgat skeldssigr slashaslth hshhslhspn svvsmhstgs
      121 ahhhledkgs spsgvlhstt nsnpstpsap stptaaadgs tgpsgngngs vsnndsaskk
      181 ppfsyvalia maiqhsaqkr atlseiyayi tarfpyfqkn kkgwqnsirh nlslnecfvk
      241 vpregggerk gnfwtldpqy admfengnyr rrrrmkrpyr napyhkplfg dpfspthvhl
      301 gprnlfghsp psyapstytr ydtsawslqq sqlsyshcqs lqpqlqpmqs mqippingys
      361 qlgtsltfqg nyldvpggta sspgsmgggs fgssfarrhd aamtpetvpt rcywpettlp
      421 nllpahchpy fndpcdeall tsqscmvnvk eepgsvtvss tsvggvgvps gmmgttaptg
      481 vstaafspme fqsrskcfm
//
LOCUS       XP_034190265             496 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608780 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190265
VERSION     XP_034190265.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334374.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="uncharacterized protein LOC117608780 isoform X2"
                     /calculated_mol_wt=53258
     Region          117..>369
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          180..268
                     /region_name="FH_FOXL2"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L2 (FOXL2) and similar proteins; cd20028"
                     /db_xref="CDD:410802"
     Site            order(185,203..204,207,226..227,229..230,233,240,250..252,
                     254)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410802"
     CDS             1..496
                     /gene="LOC117608780"
                     /coded_by="XM_034334374.2:510..2000"
                     /db_xref="GeneID:117608780"
ORIGIN      
        1 mnyadldrtr mvshvipvag elqqgngslg vtglggmqes shslkikqep fqvsppsplp
       61 slhqvssfvs dmqngclgat skeldssigr slashaslth hshhslhspn svvsmhstgs
      121 ahhhledkgs spsgvlhstt nsnpstpsap stptaaadgs tgpsgngngs vsnndsaskk
      181 ppfsyvalia maiqhsaqkr atlseiyayi tarfpyfqkn kkgwqnsirh nlslnecfvk
      241 vpregggerk gnfwtldpqy admfengnyr rrrrmkrpyr napyhkplfg dpfspthvhl
      301 gprnlfghsp psyapstytr ydtsawslqq sqlsyshcqs lqpqlqpmqs mqippingys
      361 qlgtslsnyl dvpggtassp gsmgggsfgs sfarrhdaam tpetvptrcy wpettlpnll
      421 pahchpyfnd pcdealltsq scmvnvkeep gsvtvsstsv ggvgvpsgmm gttaptgvst
      481 aafspmefqs rskcfm
//
LOCUS       XP_034190266             489 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608780 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190266
VERSION     XP_034190266.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334375.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="uncharacterized protein LOC117608780 isoform X3"
                     /calculated_mol_wt=52499
     Region          180..268
                     /region_name="FH_FOXL2"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L2 (FOXL2) and similar proteins; cd20028"
                     /db_xref="CDD:410802"
     Site            order(185,203..204,207,226..227,229..230,233,240,250..252,
                     254)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410802"
     CDS             1..489
                     /gene="LOC117608780"
                     /coded_by="XM_034334375.2:510..1979"
                     /db_xref="GeneID:117608780"
ORIGIN      
        1 mnyadldrtr mvshvipvag elqqgngslg vtglggmqes shslkikqep fqvsppsplp
       61 slhqvssfvs dmqngclgat skeldssigr slashaslth hshhslhspn svvsmhstgs
      121 ahhhledkgs spsgvlhstt nsnpstpsap stptaaadgs tgpsgngngs vsnndsaskk
      181 ppfsyvalia maiqhsaqkr atlseiyayi tarfpyfqkn kkgwqnsirh nlslnecfvk
      241 vpregggerk gnfwtldpqy admfengnyr rrrrmkrpyr napyhkplfg dpfspthvhl
      301 gprnlfghsp psyapstytr ydtsawslqq sqlsyshcqs lqpqlqpmqs mqippingys
      361 qlgtslsgta sspgsmgggs fgssfarrhd aamtpetvpt rcywpettlp nllpahchpy
      421 fndpcdeall tsqscmvnvk eepgsvtvss tsvggvgvps gmmgttaptg vstaafspme
      481 fqsrskcfm
//
LOCUS       XP_034190267             470 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608780 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190267
VERSION     XP_034190267.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334376.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="uncharacterized protein LOC117608780 isoform X4"
                     /calculated_mol_wt=50420
     Region          117..>376
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          180..268
                     /region_name="FH_FOXL2"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L2 (FOXL2) and similar proteins; cd20028"
                     /db_xref="CDD:410802"
     Site            order(185,203..204,207,226..227,229..230,233,240,250..252,
                     254)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410802"
     CDS             1..470
                     /gene="LOC117608780"
                     /coded_by="XM_034334376.2:510..1922"
                     /db_xref="GeneID:117608780"
ORIGIN      
        1 mnyadldrtr mvshvipvag elqqgngslg vtglggmqes shslkikqep fqvsppsplp
       61 slhqvssfvs dmqngclgat skeldssigr slashaslth hshhslhspn svvsmhstgs
      121 ahhhledkgs spsgvlhstt nsnpstpsap stptaaadgs tgpsgngngs vsnndsaskk
      181 ppfsyvalia maiqhsaqkr atlseiyayi tarfpyfqkn kkgwqnsirh nlslnecfvk
      241 vpregggerk gnfwtldpqy admfengnyr rrrrmkrpyr napyhkplfg dpfspthvhl
      301 gprnlfghsp psyapstytr ydtsawslqq sqlsyshcqs lqpqlqpmqs mqippingys
      361 qlgtsltfqg nyldvpggta sspgsmgggs fgssfarrhd aamtpetvpt rcywpemvnv
      421 keepgsvtvs stsvggvgvp sgmmgttapt gvstaafspm efqsrskcfm
//
LOCUS       XP_034190270             460 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608780 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190270
VERSION     XP_034190270.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334379.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="uncharacterized protein LOC117608780 isoform X5"
                     /calculated_mol_wt=49315
     Region          180..268
                     /region_name="FH_FOXL2"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L2 (FOXL2) and similar proteins; cd20028"
                     /db_xref="CDD:410802"
     Site            order(185,203..204,207,226..227,229..230,233,240,250..252,
                     254)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410802"
     CDS             1..460
                     /gene="LOC117608780"
                     /coded_by="XM_034334379.2:510..1892"
                     /db_xref="GeneID:117608780"
ORIGIN      
        1 mnyadldrtr mvshvipvag elqqgngslg vtglggmqes shslkikqep fqvsppsplp
       61 slhqvssfvs dmqngclgat skeldssigr slashaslth hshhslhspn svvsmhstgs
      121 ahhhledkgs spsgvlhstt nsnpstpsap stptaaadgs tgpsgngngs vsnndsaskk
      181 ppfsyvalia maiqhsaqkr atlseiyayi tarfpyfqkn kkgwqnsirh nlslnecfvk
      241 vpregggerk gnfwtldpqy admfengnyr rrrrmkrpyr napyhkplfg dpfspthvhl
      301 gprnlfghsp psyapstytr ydtsawslqq sqlsyshcqs lqpqlqpmqs mqippingys
      361 qlgtslsgta sspgsmgggs fgssfarrhd aamtpetvpt rcywpemvnv keepgsvtvs
      421 stsvggvgvp sgmmgttapt gvstaafspm efqsrskcfm
//
LOCUS       XP_034190271             459 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608780 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190271
VERSION     XP_034190271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334380.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="uncharacterized protein LOC117608780 isoform X6"
                     /calculated_mol_wt=49593
     Region          117..>376
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          180..268
                     /region_name="FH_FOXL2"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L2 (FOXL2) and similar proteins; cd20028"
                     /db_xref="CDD:410802"
     Site            order(185,203..204,207,226..227,229..230,233,240,250..252,
                     254)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410802"
     CDS             1..459
                     /gene="LOC117608780"
                     /coded_by="XM_034334380.2:510..1889"
                     /db_xref="GeneID:117608780"
ORIGIN      
        1 mnyadldrtr mvshvipvag elqqgngslg vtglggmqes shslkikqep fqvsppsplp
       61 slhqvssfvs dmqngclgat skeldssigr slashaslth hshhslhspn svvsmhstgs
      121 ahhhledkgs spsgvlhstt nsnpstpsap stptaaadgs tgpsgngngs vsnndsaskk
      181 ppfsyvalia maiqhsaqkr atlseiyayi tarfpyfqkn kkgwqnsirh nlslnecfvk
      241 vpregggerk gnfwtldpqy admfengnyr rrrrmkrpyr napyhkplfg dpfspthvhl
      301 gprnlfghsp psyapstytr ydtsawslqq sqlsyshcqs lqpqlqpmqs mqippingys
      361 qlgtsltfqg nyldvpggta sspgsmgggs fgssfarrhd aamtpetvpt rcywpettlp
      421 nllpahchpy fndpcdeall tsqscsnqmn tlgspnkgs
//
LOCUS       XP_034185051            1640 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185051
VERSION     XP_034185051.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329160.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185051.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1640
                     /product="uncharacterized protein LOC117606575 isoform X1"
                     /calculated_mol_wt=184830
     Region          <215..>757
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          371..1166
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1529..1639
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1536,1540,1590,1592..1593,1596..1597,1599,
                     1607..1615,1623,1625..1626,1628..1629,1632..1633,1636)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1640
                     /gene="LOC117606575"
                     /coded_by="XM_034329160.2:25..4947"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mewhgrkpvh ielpnradrf pshatpftfs rfsccgrslv trctsrlrli lrpardywtl
       61 kldtyvqars gmsvgsggra pfkrasatsi pitskstkir skpetratsg agkkrdvvas
      121 lfspkrpanr kvdrlnrhea kleeqhkgqq tqqqqpptqs qqqqqqqqnp lrqapsassl
      181 eakndnpsvg nwsslgkeas kgnakgnynp kgpgkgsrmq elereievlr kerarlesnl
      241 qeascdaqnl hdlraelasl keqhsleler laeenealra rlrdvahspl sdsekqqlll
      301 dvsrlhnsap asiaipqdeg stpvgpvpqd saqcttpdwd khssssvsev svaclqdril
      361 qmeethystn eelqatiqel sdlqaqltel qadnerltee kdvlleslcr qtekledsrs
      421 kvdtlqglll reeqpqepak gyntereqkl vdllksaqee resllqkqee ltlevkslra
      481 aaevsaaete rltkcvhlle ssveaanier kqldlelaea rqeganrsie isrlatllen
      541 arakieeleq srqvenksea delldaarre kdtletqaaa lqeqlarshc dhdrlrdqys
      601 qlqeeykvar nnaksaiddl eyrlnqlkde rlsvstelql vrdslaelqa qcqrhledkr
      661 elkaalneaq rkereaqthq yeleraldde rklrqeeiae weqfqtdllm tvrvandfkt
      721 eaqselervv menkaqrdkl rsleaqldkl nkgntaapqf ktfgnaagns rkpasnilkr
      781 grtiikrrqe skkgavrtnv lkainepgsr ktddskipnc tiprenvdsd kelfcskepe
      841 ltdklisgee vefytsentl ednektdvsa lesikdtper cspmeldntd gkvsgreice
      901 lkrtnssefm nstedypkly ipntsvpdes iaaksfllkn ikgiqnmgqn syginisnsr
      961 ffvpkdyvys gvenalddlk fevdpemkkv lqestqends aensrlittq esqksdksnl
     1021 kdkenssrlc kesensskfe dyslnkrrrl flkknesrsg seivdslldt nykqnifnst
     1081 lhnrsfstsl ddlnfhritn ssdnsdnntk kgrnkeqlti dnisksaisy kdtnnipysr
     1141 pksvqtlyrr sedldllnee qvntentcml prreknrkgi gtytekhssi lsslenlpss
     1201 vtdtsatait srltskeplk tnvrtldsla sttslsktse eealkfesdl qetrknltlc
     1261 eanrlkrmkf lnmdlsqses dteykikdsv aseetrlpks dtlsknsdck inqgivndst
     1321 saskdrslig rtsslrekfe tiiedvelrp grqigticdp nqkvtqqppq rpastptdtq
     1381 qcvltsvqqe iaarrkanis rqdsrlsvkc liesienatk qakagpgsrs sstsslnsig
     1441 tndimtlkap lrdqqqinnl ictanttnnn ktqatiarkp lsetkstvpv vlspgellds
     1501 aalnakaidf vrrnsvtdls erkdplcgli knggskrnal lkwcqnktlg yrniditnfs
     1561 sswndglalc ailhsylphk vpydtltpve krrnfsiafs aaesvgiptt lnigemcqle
     1621 rpdwqqvmty vtsiykhfet
//
LOCUS       XP_034185052            1635 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185052
VERSION     XP_034185052.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329161.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185052.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1635
                     /product="uncharacterized protein LOC117606575 isoform X2"
                     /calculated_mol_wt=184314
     Region          <215..>757
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          371..1166
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1524..1634
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1531,1535,1585,1587..1588,1591..1592,1594,
                     1602..1610,1618,1620..1621,1623..1624,1627..1628,1631)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1635
                     /gene="LOC117606575"
                     /coded_by="XM_034329161.2:25..4932"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mewhgrkpvh ielpnradrf pshatpftfs rfsccgrslv trctsrlrli lrpardywtl
       61 kldtyvqars gmsvgsggra pfkrasatsi pitskstkir skpetratsg agkkrdvvas
      121 lfspkrpanr kvdrlnrhea kleeqhkgqq tqqqqpptqs qqqqqqqqnp lrqapsassl
      181 eakndnpsvg nwsslgkeas kgnakgnynp kgpgkgsrmq elereievlr kerarlesnl
      241 qeascdaqnl hdlraelasl keqhsleler laeenealra rlrdvahspl sdsekqqlll
      301 dvsrlhnsap asiaipqdeg stpvgpvpqd saqcttpdwd khssssvsev svaclqdril
      361 qmeethystn eelqatiqel sdlqaqltel qadnerltee kdvlleslcr qtekledsrs
      421 kvdtlqglll reeqpqepak gyntereqkl vdllksaqee resllqkqee ltlevkslra
      481 aaevsaaete rltkcvhlle ssveaanier kqldlelaea rqeganrsie isrlatllen
      541 arakieeleq srqvenksea delldaarre kdtletqaaa lqeqlarshc dhdrlrdqys
      601 qlqeeykvar nnaksaiddl eyrlnqlkde rlsvstelql vrdslaelqa qcqrhledkr
      661 elkaalneaq rkereaqthq yeleraldde rklrqeeiae weqfqtdllm tvrvandfkt
      721 eaqselervv menkaqrdkl rsleaqldkl nkgntaapqf ktfgnaagns rkpasnilkr
      781 grtiikrrqe skkgavrtnv lkainepgsr ktddskipnc tiprenvdsd kelfcskepe
      841 ltdklisgee vefytsentl ednektdvsa lesikdtper cspmeldntd gkvsgreice
      901 lkrtnssefm nstedypkly ipntsvpdes iaaksfllkn ikgiqnmgqn syginisnsr
      961 ffvpkdyvys gvenalddlk fevdpemkkv lqestqends aensrlittq esqksdksnl
     1021 kdkenssrlc kesensskfe dyslnkrrrl flkknesrsg seivdslldt nykqnifnst
     1081 lhnrsfstsl ddlnfhritn ssdnsdnntk kgrnkeqlti dnisksaisy kdtnnipysr
     1141 pksvqtlyrr sedldllnee qvntentcml prreknrkgi gtytekhssi lsslenlpss
     1201 vtdtsatait srltskeplk tnvrtldsla sttslsktse eealkfesdl qetrknltlc
     1261 eanrlkrmkf lnmdlsqses dteykikdsv aseetrlpks dtlsknsdck inqgivndst
     1321 saskdrslig rtsslrekfe tiiedvelrp grqianqkvt qqppqrpast ptdtqqcvlt
     1381 svqqeiaarr kanisrqdsr lsvkcliesi enatkqakag pgsrssstss lnsigtndim
     1441 tlkaplrdqq qinnlictan ttnnnktqat iarkplsetk stvpvvlspg elldsaalna
     1501 kaidfvrrns vtdlserkdp lcgliknggs krnallkwcq nktlgyrnid itnfssswnd
     1561 glalcailhs ylphkvpydt ltpvekrrnf siafsaaesv gipttlnige mcqlerpdwq
     1621 qvmtyvtsiy khfet
//
LOCUS       XP_076545551            1617 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545551
VERSION     XP_076545551.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689436.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1617
                     /product="uncharacterized protein LOC117606575 isoform X3"
                     /calculated_mol_wt=182173
     Region          <192..>734
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          348..1143
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1506..1616
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1513,1517,1567,1569..1570,1573..1574,1576,
                     1584..1592,1600,1602..1603,1605..1606,1609..1610,1613)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1617
                     /gene="LOC117606575"
                     /coded_by="XM_076689436.1:2186..7039"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mshlrfrcct ctvntetfay vsvcteqiei wrvlydreda wendarsgms vgsggrapfk
       61 rasatsipit skstkirskp etratsgagk krdvvaslfs pkrpanrkvd rlnrheakle
      121 eqhkgqqtqq qqpptqsqqq qqqqqnplrq apsassleak ndnpsvgnws slgkeaskgn
      181 akgnynpkgp gkgsrmqele reievlrker arlesnlqea scdaqnlhdl raelaslkeq
      241 hslelerlae enealrarlr dvahsplsds ekqqllldvs rlhnsapasi aipqdegstp
      301 vgpvpqdsaq cttpdwdkhs sssvsevsva clqdrilqme ethystneel qatiqelsdl
      361 qaqltelqad nerlteekdv lleslcrqte kledsrskvd tlqglllree qpqepakgyn
      421 tereqklvdl lksaqeeres llqkqeeltl evkslraaae vsaaeterlt kcvhllessv
      481 eaanierkql dlelaearqe ganrsieisr latllenara kieeleqsrq venkseadel
      541 ldaarrekdt letqaaalqe qlarshcdhd rlrdqysqlq eeykvarnna ksaiddleyr
      601 lnqlkderls vstelqlvrd slaelqaqcq rhledkrelk aalneaqrke reaqthqyel
      661 eraldderkl rqeeiaeweq fqtdllmtvr vandfkteaq selervvmen kaqrdklrsl
      721 eaqldklnkg ntaapqfktf gnaagnsrkp asnilkrgrt iikrrqeskk gavrtnvlka
      781 inepgsrktd dskipnctip renvdsdkel fcskepeltd klisgeevef ytsentledn
      841 ektdvsales ikdtpercsp meldntdgkv sgreicelkr tnssefmnst edypklyipn
      901 tsvpdesiaa ksfllknikg iqnmgqnsyg inisnsrffv pkdyvysgve nalddlkfev
      961 dpemkkvlqe stqendsaen srlittqesq ksdksnlkdk enssrlckes ensskfedys
     1021 lnkrrrlflk knesrsgsei vdslldtnyk qnifnstlhn rsfstslddl nfhritnssd
     1081 nsdnntkkgr nkeqltidni sksaisykdt nnipysrpks vqtlyrrsed ldllneeqvn
     1141 tentcmlprr eknrkgigty tekhssilss lenlpssvtd tsataitsrl tskeplktnv
     1201 rtldslastt slsktseeea lkfesdlqet rknltlcean rlkrmkflnm dlsqsesdte
     1261 ykikdsvase etrlpksdtl sknsdckinq givndstsas kdrsligrts slrekfetii
     1321 edvelrpgrq igticdpnqk vtqqppqrpa stptdtqqcv ltsvqqeiaa rrkanisrqd
     1381 srlsvkclie sienatkqak agpgsrssst sslnsigtnd imtlkaplrd qqqinnlict
     1441 anttnnnktq atiarkplse tkstvpvvls pgelldsaal nakaidfvrr nsvtdlserk
     1501 dplcglikng gskrnallkw cqnktlgyrn iditnfsssw ndglalcail hsylphkvpy
     1561 dtltpvekrr nfsiafsaae svgipttlni gemcqlerpd wqqvmtyvts iykhfet
//
LOCUS       XP_076545552            1617 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545552
VERSION     XP_076545552.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689437.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1617
                     /product="uncharacterized protein LOC117606575 isoform X3"
                     /calculated_mol_wt=182173
     Region          <192..>734
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          348..1143
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1506..1616
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1513,1517,1567,1569..1570,1573..1574,1576,
                     1584..1592,1600,1602..1603,1605..1606,1609..1610,1613)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1617
                     /gene="LOC117606575"
                     /coded_by="XM_076689437.1:2241..7094"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mshlrfrcct ctvntetfay vsvcteqiei wrvlydreda wendarsgms vgsggrapfk
       61 rasatsipit skstkirskp etratsgagk krdvvaslfs pkrpanrkvd rlnrheakle
      121 eqhkgqqtqq qqpptqsqqq qqqqqnplrq apsassleak ndnpsvgnws slgkeaskgn
      181 akgnynpkgp gkgsrmqele reievlrker arlesnlqea scdaqnlhdl raelaslkeq
      241 hslelerlae enealrarlr dvahsplsds ekqqllldvs rlhnsapasi aipqdegstp
      301 vgpvpqdsaq cttpdwdkhs sssvsevsva clqdrilqme ethystneel qatiqelsdl
      361 qaqltelqad nerlteekdv lleslcrqte kledsrskvd tlqglllree qpqepakgyn
      421 tereqklvdl lksaqeeres llqkqeeltl evkslraaae vsaaeterlt kcvhllessv
      481 eaanierkql dlelaearqe ganrsieisr latllenara kieeleqsrq venkseadel
      541 ldaarrekdt letqaaalqe qlarshcdhd rlrdqysqlq eeykvarnna ksaiddleyr
      601 lnqlkderls vstelqlvrd slaelqaqcq rhledkrelk aalneaqrke reaqthqyel
      661 eraldderkl rqeeiaeweq fqtdllmtvr vandfkteaq selervvmen kaqrdklrsl
      721 eaqldklnkg ntaapqfktf gnaagnsrkp asnilkrgrt iikrrqeskk gavrtnvlka
      781 inepgsrktd dskipnctip renvdsdkel fcskepeltd klisgeevef ytsentledn
      841 ektdvsales ikdtpercsp meldntdgkv sgreicelkr tnssefmnst edypklyipn
      901 tsvpdesiaa ksfllknikg iqnmgqnsyg inisnsrffv pkdyvysgve nalddlkfev
      961 dpemkkvlqe stqendsaen srlittqesq ksdksnlkdk enssrlckes ensskfedys
     1021 lnkrrrlflk knesrsgsei vdslldtnyk qnifnstlhn rsfstslddl nfhritnssd
     1081 nsdnntkkgr nkeqltidni sksaisykdt nnipysrpks vqtlyrrsed ldllneeqvn
     1141 tentcmlprr eknrkgigty tekhssilss lenlpssvtd tsataitsrl tskeplktnv
     1201 rtldslastt slsktseeea lkfesdlqet rknltlcean rlkrmkflnm dlsqsesdte
     1261 ykikdsvase etrlpksdtl sknsdckinq givndstsas kdrsligrts slrekfetii
     1321 edvelrpgrq igticdpnqk vtqqppqrpa stptdtqqcv ltsvqqeiaa rrkanisrqd
     1381 srlsvkclie sienatkqak agpgsrssst sslnsigtnd imtlkaplrd qqqinnlict
     1441 anttnnnktq atiarkplse tkstvpvvls pgelldsaal nakaidfvrr nsvtdlserk
     1501 dplcglikng gskrnallkw cqnktlgyrn iditnfsssw ndglalcail hsylphkvpy
     1561 dtltpvekrr nfsiafsaae svgipttlni gemcqlerpd wqqvmtyvts iykhfet
//
LOCUS       XP_076545553            1617 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545553
VERSION     XP_076545553.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689438.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1617
                     /product="uncharacterized protein LOC117606575 isoform X3"
                     /calculated_mol_wt=182173
     Region          <192..>734
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          348..1143
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1506..1616
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1513,1517,1567,1569..1570,1573..1574,1576,
                     1584..1592,1600,1602..1603,1605..1606,1609..1610,1613)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1617
                     /gene="LOC117606575"
                     /coded_by="XM_076689438.1:2196..7049"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mshlrfrcct ctvntetfay vsvcteqiei wrvlydreda wendarsgms vgsggrapfk
       61 rasatsipit skstkirskp etratsgagk krdvvaslfs pkrpanrkvd rlnrheakle
      121 eqhkgqqtqq qqpptqsqqq qqqqqnplrq apsassleak ndnpsvgnws slgkeaskgn
      181 akgnynpkgp gkgsrmqele reievlrker arlesnlqea scdaqnlhdl raelaslkeq
      241 hslelerlae enealrarlr dvahsplsds ekqqllldvs rlhnsapasi aipqdegstp
      301 vgpvpqdsaq cttpdwdkhs sssvsevsva clqdrilqme ethystneel qatiqelsdl
      361 qaqltelqad nerlteekdv lleslcrqte kledsrskvd tlqglllree qpqepakgyn
      421 tereqklvdl lksaqeeres llqkqeeltl evkslraaae vsaaeterlt kcvhllessv
      481 eaanierkql dlelaearqe ganrsieisr latllenara kieeleqsrq venkseadel
      541 ldaarrekdt letqaaalqe qlarshcdhd rlrdqysqlq eeykvarnna ksaiddleyr
      601 lnqlkderls vstelqlvrd slaelqaqcq rhledkrelk aalneaqrke reaqthqyel
      661 eraldderkl rqeeiaeweq fqtdllmtvr vandfkteaq selervvmen kaqrdklrsl
      721 eaqldklnkg ntaapqfktf gnaagnsrkp asnilkrgrt iikrrqeskk gavrtnvlka
      781 inepgsrktd dskipnctip renvdsdkel fcskepeltd klisgeevef ytsentledn
      841 ektdvsales ikdtpercsp meldntdgkv sgreicelkr tnssefmnst edypklyipn
      901 tsvpdesiaa ksfllknikg iqnmgqnsyg inisnsrffv pkdyvysgve nalddlkfev
      961 dpemkkvlqe stqendsaen srlittqesq ksdksnlkdk enssrlckes ensskfedys
     1021 lnkrrrlflk knesrsgsei vdslldtnyk qnifnstlhn rsfstslddl nfhritnssd
     1081 nsdnntkkgr nkeqltidni sksaisykdt nnipysrpks vqtlyrrsed ldllneeqvn
     1141 tentcmlprr eknrkgigty tekhssilss lenlpssvtd tsataitsrl tskeplktnv
     1201 rtldslastt slsktseeea lkfesdlqet rknltlcean rlkrmkflnm dlsqsesdte
     1261 ykikdsvase etrlpksdtl sknsdckinq givndstsas kdrsligrts slrekfetii
     1321 edvelrpgrq igticdpnqk vtqqppqrpa stptdtqqcv ltsvqqeiaa rrkanisrqd
     1381 srlsvkclie sienatkqak agpgsrssst sslnsigtnd imtlkaplrd qqqinnlict
     1441 anttnnnktq atiarkplse tkstvpvvls pgelldsaal nakaidfvrr nsvtdlserk
     1501 dplcglikng gskrnallkw cqnktlgyrn iditnfsssw ndglalcail hsylphkvpy
     1561 dtltpvekrr nfsiafsaae svgipttlni gemcqlerpd wqqvmtyvts iykhfet
//
LOCUS       XP_076545554            1617 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545554
VERSION     XP_076545554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689439.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1617
                     /product="uncharacterized protein LOC117606575 isoform X3"
                     /calculated_mol_wt=182173
     Region          <192..>734
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          348..1143
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1506..1616
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1513,1517,1567,1569..1570,1573..1574,1576,
                     1584..1592,1600,1602..1603,1605..1606,1609..1610,1613)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1617
                     /gene="LOC117606575"
                     /coded_by="XM_076689439.1:2237..7090"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mshlrfrcct ctvntetfay vsvcteqiei wrvlydreda wendarsgms vgsggrapfk
       61 rasatsipit skstkirskp etratsgagk krdvvaslfs pkrpanrkvd rlnrheakle
      121 eqhkgqqtqq qqpptqsqqq qqqqqnplrq apsassleak ndnpsvgnws slgkeaskgn
      181 akgnynpkgp gkgsrmqele reievlrker arlesnlqea scdaqnlhdl raelaslkeq
      241 hslelerlae enealrarlr dvahsplsds ekqqllldvs rlhnsapasi aipqdegstp
      301 vgpvpqdsaq cttpdwdkhs sssvsevsva clqdrilqme ethystneel qatiqelsdl
      361 qaqltelqad nerlteekdv lleslcrqte kledsrskvd tlqglllree qpqepakgyn
      421 tereqklvdl lksaqeeres llqkqeeltl evkslraaae vsaaeterlt kcvhllessv
      481 eaanierkql dlelaearqe ganrsieisr latllenara kieeleqsrq venkseadel
      541 ldaarrekdt letqaaalqe qlarshcdhd rlrdqysqlq eeykvarnna ksaiddleyr
      601 lnqlkderls vstelqlvrd slaelqaqcq rhledkrelk aalneaqrke reaqthqyel
      661 eraldderkl rqeeiaeweq fqtdllmtvr vandfkteaq selervvmen kaqrdklrsl
      721 eaqldklnkg ntaapqfktf gnaagnsrkp asnilkrgrt iikrrqeskk gavrtnvlka
      781 inepgsrktd dskipnctip renvdsdkel fcskepeltd klisgeevef ytsentledn
      841 ektdvsales ikdtpercsp meldntdgkv sgreicelkr tnssefmnst edypklyipn
      901 tsvpdesiaa ksfllknikg iqnmgqnsyg inisnsrffv pkdyvysgve nalddlkfev
      961 dpemkkvlqe stqendsaen srlittqesq ksdksnlkdk enssrlckes ensskfedys
     1021 lnkrrrlflk knesrsgsei vdslldtnyk qnifnstlhn rsfstslddl nfhritnssd
     1081 nsdnntkkgr nkeqltidni sksaisykdt nnipysrpks vqtlyrrsed ldllneeqvn
     1141 tentcmlprr eknrkgigty tekhssilss lenlpssvtd tsataitsrl tskeplktnv
     1201 rtldslastt slsktseeea lkfesdlqet rknltlcean rlkrmkflnm dlsqsesdte
     1261 ykikdsvase etrlpksdtl sknsdckinq givndstsas kdrsligrts slrekfetii
     1321 edvelrpgrq igticdpnqk vtqqppqrpa stptdtqqcv ltsvqqeiaa rrkanisrqd
     1381 srlsvkclie sienatkqak agpgsrssst sslnsigtnd imtlkaplrd qqqinnlict
     1441 anttnnnktq atiarkplse tkstvpvvls pgelldsaal nakaidfvrr nsvtdlserk
     1501 dplcglikng gskrnallkw cqnktlgyrn iditnfsssw ndglalcail hsylphkvpy
     1561 dtltpvekrr nfsiafsaae svgipttlni gemcqlerpd wqqvmtyvts iykhfet
//
LOCUS       XP_076545555            1617 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545555
VERSION     XP_076545555.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689440.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1617
                     /product="uncharacterized protein LOC117606575 isoform X3"
                     /calculated_mol_wt=182173
     Region          <192..>734
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          348..1143
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1506..1616
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1513,1517,1567,1569..1570,1573..1574,1576,
                     1584..1592,1600,1602..1603,1605..1606,1609..1610,1613)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1617
                     /gene="LOC117606575"
                     /coded_by="XM_076689440.1:2253..7106"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mshlrfrcct ctvntetfay vsvcteqiei wrvlydreda wendarsgms vgsggrapfk
       61 rasatsipit skstkirskp etratsgagk krdvvaslfs pkrpanrkvd rlnrheakle
      121 eqhkgqqtqq qqpptqsqqq qqqqqnplrq apsassleak ndnpsvgnws slgkeaskgn
      181 akgnynpkgp gkgsrmqele reievlrker arlesnlqea scdaqnlhdl raelaslkeq
      241 hslelerlae enealrarlr dvahsplsds ekqqllldvs rlhnsapasi aipqdegstp
      301 vgpvpqdsaq cttpdwdkhs sssvsevsva clqdrilqme ethystneel qatiqelsdl
      361 qaqltelqad nerlteekdv lleslcrqte kledsrskvd tlqglllree qpqepakgyn
      421 tereqklvdl lksaqeeres llqkqeeltl evkslraaae vsaaeterlt kcvhllessv
      481 eaanierkql dlelaearqe ganrsieisr latllenara kieeleqsrq venkseadel
      541 ldaarrekdt letqaaalqe qlarshcdhd rlrdqysqlq eeykvarnna ksaiddleyr
      601 lnqlkderls vstelqlvrd slaelqaqcq rhledkrelk aalneaqrke reaqthqyel
      661 eraldderkl rqeeiaeweq fqtdllmtvr vandfkteaq selervvmen kaqrdklrsl
      721 eaqldklnkg ntaapqfktf gnaagnsrkp asnilkrgrt iikrrqeskk gavrtnvlka
      781 inepgsrktd dskipnctip renvdsdkel fcskepeltd klisgeevef ytsentledn
      841 ektdvsales ikdtpercsp meldntdgkv sgreicelkr tnssefmnst edypklyipn
      901 tsvpdesiaa ksfllknikg iqnmgqnsyg inisnsrffv pkdyvysgve nalddlkfev
      961 dpemkkvlqe stqendsaen srlittqesq ksdksnlkdk enssrlckes ensskfedys
     1021 lnkrrrlflk knesrsgsei vdslldtnyk qnifnstlhn rsfstslddl nfhritnssd
     1081 nsdnntkkgr nkeqltidni sksaisykdt nnipysrpks vqtlyrrsed ldllneeqvn
     1141 tentcmlprr eknrkgigty tekhssilss lenlpssvtd tsataitsrl tskeplktnv
     1201 rtldslastt slsktseeea lkfesdlqet rknltlcean rlkrmkflnm dlsqsesdte
     1261 ykikdsvase etrlpksdtl sknsdckinq givndstsas kdrsligrts slrekfetii
     1321 edvelrpgrq igticdpnqk vtqqppqrpa stptdtqqcv ltsvqqeiaa rrkanisrqd
     1381 srlsvkclie sienatkqak agpgsrssst sslnsigtnd imtlkaplrd qqqinnlict
     1441 anttnnnktq atiarkplse tkstvpvvls pgelldsaal nakaidfvrr nsvtdlserk
     1501 dplcglikng gskrnallkw cqnktlgyrn iditnfsssw ndglalcail hsylphkvpy
     1561 dtltpvekrr nfsiafsaae svgipttlni gemcqlerpd wqqvmtyvts iykhfet
//
LOCUS       XP_076545556            1586 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545556
VERSION     XP_076545556.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689441.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1586
                     /product="uncharacterized protein LOC117606575 isoform X4"
                     /calculated_mol_wt=178225
     Region          <161..>703
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          317..1112
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1475..1585
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1482,1486,1536,1538..1539,1542..1543,1545,
                     1553..1561,1569,1571..1572,1574..1575,1578..1579,1582)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1586
                     /gene="LOC117606575"
                     /coded_by="XM_076689441.1:2120..6880"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mlrqlldaig gnsarsgmsv gsggrapfkr asatsipits kstkirskpe tratsgagkk
       61 rdvvaslfsp krpanrkvdr lnrheaklee qhkgqqtqqq qpptqsqqqq qqqqnplrqa
      121 psassleakn dnpsvgnwss lgkeaskgna kgnynpkgpg kgsrmqeler eievlrkera
      181 rlesnlqeas cdaqnlhdlr aelaslkeqh slelerlaee nealrarlrd vahsplsdse
      241 kqqllldvsr lhnsapasia ipqdegstpv gpvpqdsaqc ttpdwdkhss ssvsevsvac
      301 lqdrilqmee thystneelq atiqelsdlq aqltelqadn erlteekdvl leslcrqtek
      361 ledsrskvdt lqglllreeq pqepakgynt ereqklvdll ksaqeeresl lqkqeeltle
      421 vkslraaaev saaeterltk cvhllessve aanierkqld lelaearqeg anrsieisrl
      481 atllenarak ieeleqsrqv enkseadell daarrekdtl etqaaalqeq larshcdhdr
      541 lrdqysqlqe eykvarnnak saiddleyrl nqlkderlsv stelqlvrds laelqaqcqr
      601 hledkrelka alneaqrker eaqthqyele raldderklr qeeiaeweqf qtdllmtvrv
      661 andfkteaqs elervvmenk aqrdklrsle aqldklnkgn taapqfktfg naagnsrkpa
      721 snilkrgrti ikrrqeskkg avrtnvlkai nepgsrktdd skipnctipr envdsdkelf
      781 cskepeltdk lisgeevefy tsentledne ktdvsalesi kdtpercspm eldntdgkvs
      841 greicelkrt nssefmnste dypklyipnt svpdesiaak sfllknikgi qnmgqnsygi
      901 nisnsrffvp kdyvysgven alddlkfevd pemkkvlqes tqendsaens rlittqesqk
      961 sdksnlkdke nssrlckese nsskfedysl nkrrrlflkk nesrsgseiv dslldtnykq
     1021 nifnstlhnr sfstslddln fhritnssdn sdnntkkgrn keqltidnis ksaisykdtn
     1081 nipysrpksv qtlyrrsedl dllneeqvnt entcmlprre knrkgigtyt ekhssilssl
     1141 enlpssvtdt sataitsrlt skeplktnvr tldslastts lsktseeeal kfesdlqetr
     1201 knltlceanr lkrmkflnmd lsqsesdtey kikdsvasee trlpksdtls knsdckinqg
     1261 ivndstsask drsligrtss lrekfetiie dvelrpgrqi gticdpnqkv tqqppqrpas
     1321 tptdtqqcvl tsvqqeiaar rkanisrqds rlsvkclies ienatkqaka gpgsrsssts
     1381 slnsigtndi mtlkaplrdq qqinnlicta nttnnnktqa tiarkplset kstvpvvlsp
     1441 gelldsaaln akaidfvrrn svtdlserkd plcglikngg skrnallkwc qnktlgyrni
     1501 ditnfssswn dglalcailh sylphkvpyd tltpvekrrn fsiafsaaes vgipttlnig
     1561 emcqlerpdw qqvmtyvtsi ykhfet
//
LOCUS       XP_034185061            1569 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185061
VERSION     XP_034185061.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329170.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185061.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1569
                     /product="uncharacterized protein LOC117606575 isoform X5"
                     /calculated_mol_wt=176484
     Region          <144..>686
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          300..1095
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1458..1568
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1465,1469,1519,1521..1522,1525..1526,1528,
                     1536..1544,1552,1554..1555,1557..1558,1561..1562,1565)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1569
                     /gene="LOC117606575"
                     /coded_by="XM_034329170.2:917..5626"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 msvgsggrap fkrasatsip itskstkirs kpetratsga gkkrdvvasl fspkrpanrk
       61 vdrlnrheak leeqhkgqqt qqqqpptqsq qqqqqqqnpl rqapsassle akndnpsvgn
      121 wsslgkeask gnakgnynpk gpgkgsrmqe lereievlrk erarlesnlq eascdaqnlh
      181 dlraelaslk eqhslelerl aeenealrar lrdvahspls dsekqqllld vsrlhnsapa
      241 siaipqdegs tpvgpvpqds aqcttpdwdk hssssvsevs vaclqdrilq meethystne
      301 elqatiqels dlqaqltelq adnerlteek dvlleslcrq tekledsrsk vdtlqglllr
      361 eeqpqepakg yntereqklv dllksaqeer esllqkqeel tlevkslraa aevsaaeter
      421 ltkcvhlles sveaanierk qldlelaear qeganrsiei srlatllena rakieeleqs
      481 rqvenksead elldaarrek dtletqaaal qeqlarshcd hdrlrdqysq lqeeykvarn
      541 naksaiddle yrlnqlkder lsvstelqlv rdslaelqaq cqrhledkre lkaalneaqr
      601 kereaqthqy eleraldder klrqeeiaew eqfqtdllmt vrvandfkte aqselervvm
      661 enkaqrdklr sleaqldkln kgntaapqfk tfgnaagnsr kpasnilkrg rtiikrrqes
      721 kkgavrtnvl kainepgsrk tddskipnct iprenvdsdk elfcskepel tdklisgeev
      781 efytsentle dnektdvsal esikdtperc spmeldntdg kvsgreicel krtnssefmn
      841 stedypklyi pntsvpdesi aaksfllkni kgiqnmgqns yginisnsrf fvpkdyvysg
      901 venalddlkf evdpemkkvl qestqendsa ensrlittqe sqksdksnlk dkenssrlck
      961 esensskfed yslnkrrrlf lkknesrsgs eivdslldtn ykqnifnstl hnrsfstsld
     1021 dlnfhritns sdnsdnntkk grnkeqltid nisksaisyk dtnnipysrp ksvqtlyrrs
     1081 edldllneeq vntentcmlp rreknrkgig tytekhssil sslenlpssv tdtsataits
     1141 rltskeplkt nvrtldslas ttslsktsee ealkfesdlq etrknltlce anrlkrmkfl
     1201 nmdlsqsesd teykikdsva seetrlpksd tlsknsdcki nqgivndsts askdrsligr
     1261 tsslrekfet iiedvelrpg rqigticdpn qkvtqqppqr pastptdtqq cvltsvqqei
     1321 aarrkanisr qdsrlsvkcl iesienatkq akagpgsrss stsslnsigt ndimtlkapl
     1381 rdqqqinnli ctanttnnnk tqatiarkpl setkstvpvv lspgelldsa alnakaidfv
     1441 rrnsvtdlse rkdplcglik nggskrnall kwcqnktlgy rniditnfss swndglalca
     1501 ilhsylphkv pydtltpvek rrnfsiafsa aesvgipttl nigemcqler pdwqqvmtyv
     1561 tsiykhfet
//
LOCUS       XP_076545557            1569 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545557
VERSION     XP_076545557.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689442.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1569
                     /product="uncharacterized protein LOC117606575 isoform X5"
                     /calculated_mol_wt=176484
     Region          <144..>686
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          300..1095
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1458..1568
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1465,1469,1519,1521..1522,1525..1526,1528,
                     1536..1544,1552,1554..1555,1557..1558,1561..1562,1565)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1569
                     /gene="LOC117606575"
                     /coded_by="XM_076689442.1:392..5101"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 msvgsggrap fkrasatsip itskstkirs kpetratsga gkkrdvvasl fspkrpanrk
       61 vdrlnrheak leeqhkgqqt qqqqpptqsq qqqqqqqnpl rqapsassle akndnpsvgn
      121 wsslgkeask gnakgnynpk gpgkgsrmqe lereievlrk erarlesnlq eascdaqnlh
      181 dlraelaslk eqhslelerl aeenealrar lrdvahspls dsekqqllld vsrlhnsapa
      241 siaipqdegs tpvgpvpqds aqcttpdwdk hssssvsevs vaclqdrilq meethystne
      301 elqatiqels dlqaqltelq adnerlteek dvlleslcrq tekledsrsk vdtlqglllr
      361 eeqpqepakg yntereqklv dllksaqeer esllqkqeel tlevkslraa aevsaaeter
      421 ltkcvhlles sveaanierk qldlelaear qeganrsiei srlatllena rakieeleqs
      481 rqvenksead elldaarrek dtletqaaal qeqlarshcd hdrlrdqysq lqeeykvarn
      541 naksaiddle yrlnqlkder lsvstelqlv rdslaelqaq cqrhledkre lkaalneaqr
      601 kereaqthqy eleraldder klrqeeiaew eqfqtdllmt vrvandfkte aqselervvm
      661 enkaqrdklr sleaqldkln kgntaapqfk tfgnaagnsr kpasnilkrg rtiikrrqes
      721 kkgavrtnvl kainepgsrk tddskipnct iprenvdsdk elfcskepel tdklisgeev
      781 efytsentle dnektdvsal esikdtperc spmeldntdg kvsgreicel krtnssefmn
      841 stedypklyi pntsvpdesi aaksfllkni kgiqnmgqns yginisnsrf fvpkdyvysg
      901 venalddlkf evdpemkkvl qestqendsa ensrlittqe sqksdksnlk dkenssrlck
      961 esensskfed yslnkrrrlf lkknesrsgs eivdslldtn ykqnifnstl hnrsfstsld
     1021 dlnfhritns sdnsdnntkk grnkeqltid nisksaisyk dtnnipysrp ksvqtlyrrs
     1081 edldllneeq vntentcmlp rreknrkgig tytekhssil sslenlpssv tdtsataits
     1141 rltskeplkt nvrtldslas ttslsktsee ealkfesdlq etrknltlce anrlkrmkfl
     1201 nmdlsqsesd teykikdsva seetrlpksd tlsknsdcki nqgivndsts askdrsligr
     1261 tsslrekfet iiedvelrpg rqigticdpn qkvtqqppqr pastptdtqq cvltsvqqei
     1321 aarrkanisr qdsrlsvkcl iesienatkq akagpgsrss stsslnsigt ndimtlkapl
     1381 rdqqqinnli ctanttnnnk tqatiarkpl setkstvpvv lspgelldsa alnakaidfv
     1441 rrnsvtdlse rkdplcglik nggskrnall kwcqnktlgy rniditnfss swndglalca
     1501 ilhsylphkv pydtltpvek rrnfsiafsa aesvgipttl nigemcqler pdwqqvmtyv
     1561 tsiykhfet
//
LOCUS       XP_034185063            1503 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185063
VERSION     XP_034185063.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185063.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="uncharacterized protein LOC117606575 isoform X6"
                     /calculated_mol_wt=169528
     Region          <78..>620
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          234..1029
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1392..1502
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1399,1403,1453,1455..1456,1459..1460,1462,
                     1470..1478,1486,1488..1489,1491..1492,1495..1496,1499)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1503
                     /gene="LOC117606575"
                     /coded_by="XM_034329172.2:4021..8532"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mmkfkslfrr gqqtqqqqpp tqsqqqqqqq qnplrqapsa ssleakndnp svgnwsslgk
       61 easkgnakgn ynpkgpgkgs rmqelereie vlrkerarle snlqeascda qnlhdlrael
      121 aslkeqhsle lerlaeenea lrarlrdvah splsdsekqq llldvsrlhn sapasiaipq
      181 degstpvgpv pqdsaqcttp dwdkhssssv sevsvaclqd rilqmeethy stneelqati
      241 qelsdlqaql telqadnerl teekdvlles lcrqtekled srskvdtlqg lllreeqpqe
      301 pakgyntere qklvdllksa qeeresllqk qeeltlevks lraaaevsaa eterltkcvh
      361 llessveaan ierkqldlel aearqeganr sieisrlatl lenarakiee leqsrqvenk
      421 seadelldaa rrekdtletq aaalqeqlar shcdhdrlrd qysqlqeeyk varnnaksai
      481 ddleyrlnql kderlsvste lqlvrdslae lqaqcqrhle dkrelkaaln eaqrkereaq
      541 thqyeleral dderklrqee iaeweqfqtd llmtvrvand fkteaqsele rvvmenkaqr
      601 dklrsleaql dklnkgntaa pqfktfgnaa gnsrkpasni lkrgrtiikr rqeskkgavr
      661 tnvlkainep gsrktddski pnctiprenv dsdkelfcsk epeltdklis geevefytse
      721 ntlednektd vsalesikdt percspmeld ntdgkvsgre icelkrtnss efmnstedyp
      781 klyipntsvp desiaaksfl lknikgiqnm gqnsyginis nsrffvpkdy vysgvenald
      841 dlkfevdpem kkvlqestqe ndsaensrli ttqesqksdk snlkdkenss rlckesenss
      901 kfedyslnkr rrlflkknes rsgseivdsl ldtnykqnif nstlhnrsfs tslddlnfhr
      961 itnssdnsdn ntkkgrnkeq ltidnisksa isykdtnnip ysrpksvqtl yrrsedldll
     1021 neeqvntent cmlprreknr kgigtytekh ssilsslenl pssvtdtsat aitsrltske
     1081 plktnvrtld slasttslsk tseeealkfe sdlqetrknl tlceanrlkr mkflnmdlsq
     1141 sesdteykik dsvaseetrl pksdtlskns dckinqgivn dstsaskdrs ligrtsslre
     1201 kfetiiedve lrpgrqigti cdpnqkvtqq ppqrpastpt dtqqcvltsv qqeiaarrka
     1261 nisrqdsrls vkcliesien atkqakagpg srssstssln sigtndimtl kaplrdqqqi
     1321 nnlictantt nnnktqatia rkplsetkst vpvvlspgel ldsaalnaka idfvrrnsvt
     1381 dlserkdplc gliknggskr nallkwcqnk tlgyrnidit nfssswndgl alcailhsyl
     1441 phkvpydtlt pvekrrnfsi afsaaesvgi pttlnigemc qlerpdwqqv mtyvtsiykh
     1501 fet
//
LOCUS       XP_076545558            1503 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545558
VERSION     XP_076545558.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689443.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="uncharacterized protein LOC117606575 isoform X6"
                     /calculated_mol_wt=169528
     Region          <78..>620
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          234..1029
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1392..1502
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1399,1403,1453,1455..1456,1459..1460,1462,
                     1470..1478,1486,1488..1489,1491..1492,1495..1496,1499)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1503
                     /gene="LOC117606575"
                     /coded_by="XM_076689443.1:4289..8800"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mmkfkslfrr gqqtqqqqpp tqsqqqqqqq qnplrqapsa ssleakndnp svgnwsslgk
       61 easkgnakgn ynpkgpgkgs rmqelereie vlrkerarle snlqeascda qnlhdlrael
      121 aslkeqhsle lerlaeenea lrarlrdvah splsdsekqq llldvsrlhn sapasiaipq
      181 degstpvgpv pqdsaqcttp dwdkhssssv sevsvaclqd rilqmeethy stneelqati
      241 qelsdlqaql telqadnerl teekdvlles lcrqtekled srskvdtlqg lllreeqpqe
      301 pakgyntere qklvdllksa qeeresllqk qeeltlevks lraaaevsaa eterltkcvh
      361 llessveaan ierkqldlel aearqeganr sieisrlatl lenarakiee leqsrqvenk
      421 seadelldaa rrekdtletq aaalqeqlar shcdhdrlrd qysqlqeeyk varnnaksai
      481 ddleyrlnql kderlsvste lqlvrdslae lqaqcqrhle dkrelkaaln eaqrkereaq
      541 thqyeleral dderklrqee iaeweqfqtd llmtvrvand fkteaqsele rvvmenkaqr
      601 dklrsleaql dklnkgntaa pqfktfgnaa gnsrkpasni lkrgrtiikr rqeskkgavr
      661 tnvlkainep gsrktddski pnctiprenv dsdkelfcsk epeltdklis geevefytse
      721 ntlednektd vsalesikdt percspmeld ntdgkvsgre icelkrtnss efmnstedyp
      781 klyipntsvp desiaaksfl lknikgiqnm gqnsyginis nsrffvpkdy vysgvenald
      841 dlkfevdpem kkvlqestqe ndsaensrli ttqesqksdk snlkdkenss rlckesenss
      901 kfedyslnkr rrlflkknes rsgseivdsl ldtnykqnif nstlhnrsfs tslddlnfhr
      961 itnssdnsdn ntkkgrnkeq ltidnisksa isykdtnnip ysrpksvqtl yrrsedldll
     1021 neeqvntent cmlprreknr kgigtytekh ssilsslenl pssvtdtsat aitsrltske
     1081 plktnvrtld slasttslsk tseeealkfe sdlqetrknl tlceanrlkr mkflnmdlsq
     1141 sesdteykik dsvaseetrl pksdtlskns dckinqgivn dstsaskdrs ligrtsslre
     1201 kfetiiedve lrpgrqigti cdpnqkvtqq ppqrpastpt dtqqcvltsv qqeiaarrka
     1261 nisrqdsrls vkcliesien atkqakagpg srssstssln sigtndimtl kaplrdqqqi
     1321 nnlictantt nnnktqatia rkplsetkst vpvvlspgel ldsaalnaka idfvrrnsvt
     1381 dlserkdplc gliknggskr nallkwcqnk tlgyrnidit nfssswndgl alcailhsyl
     1441 phkvpydtlt pvekrrnfsi afsaaesvgi pttlnigemc qlerpdwqqv mtyvtsiykh
     1501 fet
//
LOCUS       XP_076545559            1503 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545559
VERSION     XP_076545559.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689444.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="uncharacterized protein LOC117606575 isoform X6"
                     /calculated_mol_wt=169528
     Region          <78..>620
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          234..1029
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1392..1502
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1399,1403,1453,1455..1456,1459..1460,1462,
                     1470..1478,1486,1488..1489,1491..1492,1495..1496,1499)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1503
                     /gene="LOC117606575"
                     /coded_by="XM_076689444.1:5119..9630"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mmkfkslfrr gqqtqqqqpp tqsqqqqqqq qnplrqapsa ssleakndnp svgnwsslgk
       61 easkgnakgn ynpkgpgkgs rmqelereie vlrkerarle snlqeascda qnlhdlrael
      121 aslkeqhsle lerlaeenea lrarlrdvah splsdsekqq llldvsrlhn sapasiaipq
      181 degstpvgpv pqdsaqcttp dwdkhssssv sevsvaclqd rilqmeethy stneelqati
      241 qelsdlqaql telqadnerl teekdvlles lcrqtekled srskvdtlqg lllreeqpqe
      301 pakgyntere qklvdllksa qeeresllqk qeeltlevks lraaaevsaa eterltkcvh
      361 llessveaan ierkqldlel aearqeganr sieisrlatl lenarakiee leqsrqvenk
      421 seadelldaa rrekdtletq aaalqeqlar shcdhdrlrd qysqlqeeyk varnnaksai
      481 ddleyrlnql kderlsvste lqlvrdslae lqaqcqrhle dkrelkaaln eaqrkereaq
      541 thqyeleral dderklrqee iaeweqfqtd llmtvrvand fkteaqsele rvvmenkaqr
      601 dklrsleaql dklnkgntaa pqfktfgnaa gnsrkpasni lkrgrtiikr rqeskkgavr
      661 tnvlkainep gsrktddski pnctiprenv dsdkelfcsk epeltdklis geevefytse
      721 ntlednektd vsalesikdt percspmeld ntdgkvsgre icelkrtnss efmnstedyp
      781 klyipntsvp desiaaksfl lknikgiqnm gqnsyginis nsrffvpkdy vysgvenald
      841 dlkfevdpem kkvlqestqe ndsaensrli ttqesqksdk snlkdkenss rlckesenss
      901 kfedyslnkr rrlflkknes rsgseivdsl ldtnykqnif nstlhnrsfs tslddlnfhr
      961 itnssdnsdn ntkkgrnkeq ltidnisksa isykdtnnip ysrpksvqtl yrrsedldll
     1021 neeqvntent cmlprreknr kgigtytekh ssilsslenl pssvtdtsat aitsrltske
     1081 plktnvrtld slasttslsk tseeealkfe sdlqetrknl tlceanrlkr mkflnmdlsq
     1141 sesdteykik dsvaseetrl pksdtlskns dckinqgivn dstsaskdrs ligrtsslre
     1201 kfetiiedve lrpgrqigti cdpnqkvtqq ppqrpastpt dtqqcvltsv qqeiaarrka
     1261 nisrqdsrls vkcliesien atkqakagpg srssstssln sigtndimtl kaplrdqqqi
     1321 nnlictantt nnnktqatia rkplsetkst vpvvlspgel ldsaalnaka idfvrrnsvt
     1381 dlserkdplc gliknggskr nallkwcqnk tlgyrnidit nfssswndgl alcailhsyl
     1441 phkvpydtlt pvekrrnfsi afsaaesvgi pttlnigemc qlerpdwqqv mtyvtsiykh
     1501 fet
//
LOCUS       XP_076545560            1503 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545560
VERSION     XP_076545560.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689445.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="uncharacterized protein LOC117606575 isoform X6"
                     /calculated_mol_wt=169528
     Region          <78..>620
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          234..1029
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1392..1502
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1399,1403,1453,1455..1456,1459..1460,1462,
                     1470..1478,1486,1488..1489,1491..1492,1495..1496,1499)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1503
                     /gene="LOC117606575"
                     /coded_by="XM_076689445.1:3950..8461"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mmkfkslfrr gqqtqqqqpp tqsqqqqqqq qnplrqapsa ssleakndnp svgnwsslgk
       61 easkgnakgn ynpkgpgkgs rmqelereie vlrkerarle snlqeascda qnlhdlrael
      121 aslkeqhsle lerlaeenea lrarlrdvah splsdsekqq llldvsrlhn sapasiaipq
      181 degstpvgpv pqdsaqcttp dwdkhssssv sevsvaclqd rilqmeethy stneelqati
      241 qelsdlqaql telqadnerl teekdvlles lcrqtekled srskvdtlqg lllreeqpqe
      301 pakgyntere qklvdllksa qeeresllqk qeeltlevks lraaaevsaa eterltkcvh
      361 llessveaan ierkqldlel aearqeganr sieisrlatl lenarakiee leqsrqvenk
      421 seadelldaa rrekdtletq aaalqeqlar shcdhdrlrd qysqlqeeyk varnnaksai
      481 ddleyrlnql kderlsvste lqlvrdslae lqaqcqrhle dkrelkaaln eaqrkereaq
      541 thqyeleral dderklrqee iaeweqfqtd llmtvrvand fkteaqsele rvvmenkaqr
      601 dklrsleaql dklnkgntaa pqfktfgnaa gnsrkpasni lkrgrtiikr rqeskkgavr
      661 tnvlkainep gsrktddski pnctiprenv dsdkelfcsk epeltdklis geevefytse
      721 ntlednektd vsalesikdt percspmeld ntdgkvsgre icelkrtnss efmnstedyp
      781 klyipntsvp desiaaksfl lknikgiqnm gqnsyginis nsrffvpkdy vysgvenald
      841 dlkfevdpem kkvlqestqe ndsaensrli ttqesqksdk snlkdkenss rlckesenss
      901 kfedyslnkr rrlflkknes rsgseivdsl ldtnykqnif nstlhnrsfs tslddlnfhr
      961 itnssdnsdn ntkkgrnkeq ltidnisksa isykdtnnip ysrpksvqtl yrrsedldll
     1021 neeqvntent cmlprreknr kgigtytekh ssilsslenl pssvtdtsat aitsrltske
     1081 plktnvrtld slasttslsk tseeealkfe sdlqetrknl tlceanrlkr mkflnmdlsq
     1141 sesdteykik dsvaseetrl pksdtlskns dckinqgivn dstsaskdrs ligrtsslre
     1201 kfetiiedve lrpgrqigti cdpnqkvtqq ppqrpastpt dtqqcvltsv qqeiaarrka
     1261 nisrqdsrls vkcliesien atkqakagpg srssstssln sigtndimtl kaplrdqqqi
     1321 nnlictantt nnnktqatia rkplsetkst vpvvlspgel ldsaalnaka idfvrrnsvt
     1381 dlserkdplc gliknggskr nallkwcqnk tlgyrnidit nfssswndgl alcailhsyl
     1441 phkvpydtlt pvekrrnfsi afsaaesvgi pttlnigemc qlerpdwqqv mtyvtsiykh
     1501 fet
//
LOCUS       XP_034185064            1503 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185064
VERSION     XP_034185064.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329173.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185064.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="uncharacterized protein LOC117606575 isoform X6"
                     /calculated_mol_wt=169528
     Region          <78..>620
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          234..1029
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1392..1502
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1399,1403,1453,1455..1456,1459..1460,1462,
                     1470..1478,1486,1488..1489,1491..1492,1495..1496,1499)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1503
                     /gene="LOC117606575"
                     /coded_by="XM_034329173.2:4111..8622"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mmkfkslfrr gqqtqqqqpp tqsqqqqqqq qnplrqapsa ssleakndnp svgnwsslgk
       61 easkgnakgn ynpkgpgkgs rmqelereie vlrkerarle snlqeascda qnlhdlrael
      121 aslkeqhsle lerlaeenea lrarlrdvah splsdsekqq llldvsrlhn sapasiaipq
      181 degstpvgpv pqdsaqcttp dwdkhssssv sevsvaclqd rilqmeethy stneelqati
      241 qelsdlqaql telqadnerl teekdvlles lcrqtekled srskvdtlqg lllreeqpqe
      301 pakgyntere qklvdllksa qeeresllqk qeeltlevks lraaaevsaa eterltkcvh
      361 llessveaan ierkqldlel aearqeganr sieisrlatl lenarakiee leqsrqvenk
      421 seadelldaa rrekdtletq aaalqeqlar shcdhdrlrd qysqlqeeyk varnnaksai
      481 ddleyrlnql kderlsvste lqlvrdslae lqaqcqrhle dkrelkaaln eaqrkereaq
      541 thqyeleral dderklrqee iaeweqfqtd llmtvrvand fkteaqsele rvvmenkaqr
      601 dklrsleaql dklnkgntaa pqfktfgnaa gnsrkpasni lkrgrtiikr rqeskkgavr
      661 tnvlkainep gsrktddski pnctiprenv dsdkelfcsk epeltdklis geevefytse
      721 ntlednektd vsalesikdt percspmeld ntdgkvsgre icelkrtnss efmnstedyp
      781 klyipntsvp desiaaksfl lknikgiqnm gqnsyginis nsrffvpkdy vysgvenald
      841 dlkfevdpem kkvlqestqe ndsaensrli ttqesqksdk snlkdkenss rlckesenss
      901 kfedyslnkr rrlflkknes rsgseivdsl ldtnykqnif nstlhnrsfs tslddlnfhr
      961 itnssdnsdn ntkkgrnkeq ltidnisksa isykdtnnip ysrpksvqtl yrrsedldll
     1021 neeqvntent cmlprreknr kgigtytekh ssilsslenl pssvtdtsat aitsrltske
     1081 plktnvrtld slasttslsk tseeealkfe sdlqetrknl tlceanrlkr mkflnmdlsq
     1141 sesdteykik dsvaseetrl pksdtlskns dckinqgivn dstsaskdrs ligrtsslre
     1201 kfetiiedve lrpgrqigti cdpnqkvtqq ppqrpastpt dtqqcvltsv qqeiaarrka
     1261 nisrqdsrls vkcliesien atkqakagpg srssstssln sigtndimtl kaplrdqqqi
     1321 nnlictantt nnnktqatia rkplsetkst vpvvlspgel ldsaalnaka idfvrrnsvt
     1381 dlserkdplc gliknggskr nallkwcqnk tlgyrnidit nfssswndgl alcailhsyl
     1441 phkvpydtlt pvekrrnfsi afsaaesvgi pttlnigemc qlerpdwqqv mtyvtsiykh
     1501 fet
//
LOCUS       XP_034185065            1503 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185065
VERSION     XP_034185065.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329174.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185065.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="uncharacterized protein LOC117606575 isoform X6"
                     /calculated_mol_wt=169528
     Region          <78..>620
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          234..1029
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1392..1502
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1399,1403,1453,1455..1456,1459..1460,1462,
                     1470..1478,1486,1488..1489,1491..1492,1495..1496,1499)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1503
                     /gene="LOC117606575"
                     /coded_by="XM_034329174.2:4119..8630"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mmkfkslfrr gqqtqqqqpp tqsqqqqqqq qnplrqapsa ssleakndnp svgnwsslgk
       61 easkgnakgn ynpkgpgkgs rmqelereie vlrkerarle snlqeascda qnlhdlrael
      121 aslkeqhsle lerlaeenea lrarlrdvah splsdsekqq llldvsrlhn sapasiaipq
      181 degstpvgpv pqdsaqcttp dwdkhssssv sevsvaclqd rilqmeethy stneelqati
      241 qelsdlqaql telqadnerl teekdvlles lcrqtekled srskvdtlqg lllreeqpqe
      301 pakgyntere qklvdllksa qeeresllqk qeeltlevks lraaaevsaa eterltkcvh
      361 llessveaan ierkqldlel aearqeganr sieisrlatl lenarakiee leqsrqvenk
      421 seadelldaa rrekdtletq aaalqeqlar shcdhdrlrd qysqlqeeyk varnnaksai
      481 ddleyrlnql kderlsvste lqlvrdslae lqaqcqrhle dkrelkaaln eaqrkereaq
      541 thqyeleral dderklrqee iaeweqfqtd llmtvrvand fkteaqsele rvvmenkaqr
      601 dklrsleaql dklnkgntaa pqfktfgnaa gnsrkpasni lkrgrtiikr rqeskkgavr
      661 tnvlkainep gsrktddski pnctiprenv dsdkelfcsk epeltdklis geevefytse
      721 ntlednektd vsalesikdt percspmeld ntdgkvsgre icelkrtnss efmnstedyp
      781 klyipntsvp desiaaksfl lknikgiqnm gqnsyginis nsrffvpkdy vysgvenald
      841 dlkfevdpem kkvlqestqe ndsaensrli ttqesqksdk snlkdkenss rlckesenss
      901 kfedyslnkr rrlflkknes rsgseivdsl ldtnykqnif nstlhnrsfs tslddlnfhr
      961 itnssdnsdn ntkkgrnkeq ltidnisksa isykdtnnip ysrpksvqtl yrrsedldll
     1021 neeqvntent cmlprreknr kgigtytekh ssilsslenl pssvtdtsat aitsrltske
     1081 plktnvrtld slasttslsk tseeealkfe sdlqetrknl tlceanrlkr mkflnmdlsq
     1141 sesdteykik dsvaseetrl pksdtlskns dckinqgivn dstsaskdrs ligrtsslre
     1201 kfetiiedve lrpgrqigti cdpnqkvtqq ppqrpastpt dtqqcvltsv qqeiaarrka
     1261 nisrqdsrls vkcliesien atkqakagpg srssstssln sigtndimtl kaplrdqqqi
     1321 nnlictantt nnnktqatia rkplsetkst vpvvlspgel ldsaalnaka idfvrrnsvt
     1381 dlserkdplc gliknggskr nallkwcqnk tlgyrnidit nfssswndgl alcailhsyl
     1441 phkvpydtlt pvekrrnfsi afsaaesvgi pttlnigemc qlerpdwqqv mtyvtsiykh
     1501 fet
//
LOCUS       XP_076545561            1503 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545561
VERSION     XP_076545561.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689446.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="uncharacterized protein LOC117606575 isoform X6"
                     /calculated_mol_wt=169528
     Region          <78..>620
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          234..1029
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1392..1502
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1399,1403,1453,1455..1456,1459..1460,1462,
                     1470..1478,1486,1488..1489,1491..1492,1495..1496,1499)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1503
                     /gene="LOC117606575"
                     /coded_by="XM_076689446.1:3871..8382"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mmkfkslfrr gqqtqqqqpp tqsqqqqqqq qnplrqapsa ssleakndnp svgnwsslgk
       61 easkgnakgn ynpkgpgkgs rmqelereie vlrkerarle snlqeascda qnlhdlrael
      121 aslkeqhsle lerlaeenea lrarlrdvah splsdsekqq llldvsrlhn sapasiaipq
      181 degstpvgpv pqdsaqcttp dwdkhssssv sevsvaclqd rilqmeethy stneelqati
      241 qelsdlqaql telqadnerl teekdvlles lcrqtekled srskvdtlqg lllreeqpqe
      301 pakgyntere qklvdllksa qeeresllqk qeeltlevks lraaaevsaa eterltkcvh
      361 llessveaan ierkqldlel aearqeganr sieisrlatl lenarakiee leqsrqvenk
      421 seadelldaa rrekdtletq aaalqeqlar shcdhdrlrd qysqlqeeyk varnnaksai
      481 ddleyrlnql kderlsvste lqlvrdslae lqaqcqrhle dkrelkaaln eaqrkereaq
      541 thqyeleral dderklrqee iaeweqfqtd llmtvrvand fkteaqsele rvvmenkaqr
      601 dklrsleaql dklnkgntaa pqfktfgnaa gnsrkpasni lkrgrtiikr rqeskkgavr
      661 tnvlkainep gsrktddski pnctiprenv dsdkelfcsk epeltdklis geevefytse
      721 ntlednektd vsalesikdt percspmeld ntdgkvsgre icelkrtnss efmnstedyp
      781 klyipntsvp desiaaksfl lknikgiqnm gqnsyginis nsrffvpkdy vysgvenald
      841 dlkfevdpem kkvlqestqe ndsaensrli ttqesqksdk snlkdkenss rlckesenss
      901 kfedyslnkr rrlflkknes rsgseivdsl ldtnykqnif nstlhnrsfs tslddlnfhr
      961 itnssdnsdn ntkkgrnkeq ltidnisksa isykdtnnip ysrpksvqtl yrrsedldll
     1021 neeqvntent cmlprreknr kgigtytekh ssilsslenl pssvtdtsat aitsrltske
     1081 plktnvrtld slasttslsk tseeealkfe sdlqetrknl tlceanrlkr mkflnmdlsq
     1141 sesdteykik dsvaseetrl pksdtlskns dckinqgivn dstsaskdrs ligrtsslre
     1201 kfetiiedve lrpgrqigti cdpnqkvtqq ppqrpastpt dtqqcvltsv qqeiaarrka
     1261 nisrqdsrls vkcliesien atkqakagpg srssstssln sigtndimtl kaplrdqqqi
     1321 nnlictantt nnnktqatia rkplsetkst vpvvlspgel ldsaalnaka idfvrrnsvt
     1381 dlserkdplc gliknggskr nallkwcqnk tlgyrnidit nfssswndgl alcailhsyl
     1441 phkvpydtlt pvekrrnfsi afsaaesvgi pttlnigemc qlerpdwqqv mtyvtsiykh
     1501 fet
//
LOCUS       XP_034185071            1389 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185071
VERSION     XP_034185071.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329180.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185071.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1389
                     /product="uncharacterized protein LOC117606575 isoform X7"
                     /calculated_mol_wt=156870
     Region          120..915
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <192..508
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1278..1388
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1285,1289,1339,1341..1342,1345..1346,1348,
                     1356..1364,1372,1374..1375,1377..1378,1381..1382,1385)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1389
                     /gene="LOC117606575"
                     /coded_by="XM_034329180.2:119..4288"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mkkrcfswst eqhslelerl aeenealrar lrdvahspls dsekqqllld vsrlhnsapa
       61 siaipqdegs tpvgpvpqds aqcttpdwdk hssssvsevs vaclqdrilq meethystne
      121 elqatiqels dlqaqltelq adnerlteek dvlleslcrq tekledsrsk vdtlqglllr
      181 eeqpqepakg yntereqklv dllksaqeer esllqkqeel tlevkslraa aevsaaeter
      241 ltkcvhlles sveaanierk qldlelaear qeganrsiei srlatllena rakieeleqs
      301 rqvenksead elldaarrek dtletqaaal qeqlarshcd hdrlrdqysq lqeeykvarn
      361 naksaiddle yrlnqlkder lsvstelqlv rdslaelqaq cqrhledkre lkaalneaqr
      421 kereaqthqy eleraldder klrqeeiaew eqfqtdllmt vrvandfkte aqselervvm
      481 enkaqrdklr sleaqldkln kgntaapqfk tfgnaagnsr kpasnilkrg rtiikrrqes
      541 kkgavrtnvl kainepgsrk tddskipnct iprenvdsdk elfcskepel tdklisgeev
      601 efytsentle dnektdvsal esikdtperc spmeldntdg kvsgreicel krtnssefmn
      661 stedypklyi pntsvpdesi aaksfllkni kgiqnmgqns yginisnsrf fvpkdyvysg
      721 venalddlkf evdpemkkvl qestqendsa ensrlittqe sqksdksnlk dkenssrlck
      781 esensskfed yslnkrrrlf lkknesrsgs eivdslldtn ykqnifnstl hnrsfstsld
      841 dlnfhritns sdnsdnntkk grnkeqltid nisksaisyk dtnnipysrp ksvqtlyrrs
      901 edldllneeq vntentcmlp rreknrkgig tytekhssil sslenlpssv tdtsataits
      961 rltskeplkt nvrtldslas ttslsktsee ealkfesdlq etrknltlce anrlkrmkfl
     1021 nmdlsqsesd teykikdsva seetrlpksd tlsknsdcki nqgivndsts askdrsligr
     1081 tsslrekfet iiedvelrpg rqigticdpn qkvtqqppqr pastptdtqq cvltsvqqei
     1141 aarrkanisr qdsrlsvkcl iesienatkq akagpgsrss stsslnsigt ndimtlkapl
     1201 rdqqqinnli ctanttnnnk tqatiarkpl setkstvpvv lspgelldsa alnakaidfv
     1261 rrnsvtdlse rkdplcglik nggskrnall kwcqnktlgy rniditnfss swndglalca
     1321 ilhsylphkv pydtltpvek rrnfsiafsa aesvgipttl nigemcqler pdwqqvmtyv
     1381 tsiykhfet
//
LOCUS       XP_076545563            1581 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606575 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545563
VERSION     XP_076545563.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689448.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1581
                     /product="uncharacterized protein LOC117606575 isoform X8"
                     /calculated_mol_wt=177710
     Region          <161..>703
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          317..1112
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          1470..1580
                     /region_name="CH_CYTS"
                     /note="calponin homology (CH) domain found in the cytospin
                     family; cd21199"
                     /db_xref="CDD:409048"
     Site            order(1477,1481,1531,1533..1534,1537..1538,1540,
                     1548..1556,1564,1566..1567,1569..1570,1573..1574,1577)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409048"
     CDS             1..1581
                     /gene="LOC117606575"
                     /coded_by="XM_076689448.1:2119..6864"
                     /db_xref="GeneID:117606575"
ORIGIN      
        1 mlrqlldaig gnsarsgmsv gsggrapfkr asatsipits kstkirskpe tratsgagkk
       61 rdvvaslfsp krpanrkvdr lnrheaklee qhkgqqtqqq qpptqsqqqq qqqqnplrqa
      121 psassleakn dnpsvgnwss lgkeaskgna kgnynpkgpg kgsrmqeler eievlrkera
      181 rlesnlqeas cdaqnlhdlr aelaslkeqh slelerlaee nealrarlrd vahsplsdse
      241 kqqllldvsr lhnsapasia ipqdegstpv gpvpqdsaqc ttpdwdkhss ssvsevsvac
      301 lqdrilqmee thystneelq atiqelsdlq aqltelqadn erlteekdvl leslcrqtek
      361 ledsrskvdt lqglllreeq pqepakgynt ereqklvdll ksaqeeresl lqkqeeltle
      421 vkslraaaev saaeterltk cvhllessve aanierkqld lelaearqeg anrsieisrl
      481 atllenarak ieeleqsrqv enkseadell daarrekdtl etqaaalqeq larshcdhdr
      541 lrdqysqlqe eykvarnnak saiddleyrl nqlkderlsv stelqlvrds laelqaqcqr
      601 hledkrelka alneaqrker eaqthqyele raldderklr qeeiaeweqf qtdllmtvrv
      661 andfkteaqs elervvmenk aqrdklrsle aqldklnkgn taapqfktfg naagnsrkpa
      721 snilkrgrti ikrrqeskkg avrtnvlkai nepgsrktdd skipnctipr envdsdkelf
      781 cskepeltdk lisgeevefy tsentledne ktdvsalesi kdtpercspm eldntdgkvs
      841 greicelkrt nssefmnste dypklyipnt svpdesiaak sfllknikgi qnmgqnsygi
      901 nisnsrffvp kdyvysgven alddlkfevd pemkkvlqes tqendsaens rlittqesqk
      961 sdksnlkdke nssrlckese nsskfedysl nkrrrlflkk nesrsgseiv dslldtnykq
     1021 nifnstlhnr sfstslddln fhritnssdn sdnntkkgrn keqltidnis ksaisykdtn
     1081 nipysrpksv qtlyrrsedl dllneeqvnt entcmlprre knrkgigtyt ekhssilssl
     1141 enlpssvtdt sataitsrlt skeplktnvr tldslastts lsktseeeal kfesdlqetr
     1201 knltlceanr lkrmkflnmd lsqsesdtey kikdsvasee trlpksdtls knsdckinqg
     1261 ivndstsask drsligrtss lrekfetiie dvelrpgrqi anqkvtqqpp qrpastptdt
     1321 qqcvltsvqq eiaarrkani srqdsrlsvk cliesienat kqakagpgsr ssstsslnsi
     1381 gtndimtlka plrdqqqinn lictanttnn nktqatiark plsetkstvp vvlspgelld
     1441 saalnakaid fvrrnsvtdl serkdplcgl iknggskrna llkwcqnktl gyrniditnf
     1501 ssswndglal cailhsylph kvpydtltpv ekrrnfsiaf saaesvgipt tlnigemcql
     1561 erpdwqqvmt yvtsiykhfe t
//
LOCUS       XP_034185073            1294 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606576 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185073
VERSION     XP_034185073.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185073.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1294
                     /product="uncharacterized protein LOC117606576 isoform X1"
                     /calculated_mol_wt=143389
     Region          63..1281
                     /region_name="PLN02906"
                     /note="xanthine dehydrogenase"
                     /db_xref="CDD:215491"
     CDS             1..1294
                     /gene="LOC117606576"
                     /coded_by="XM_034329182.2:191..4075"
                     /db_xref="GeneID:117606576"
ORIGIN      
        1 mecikrsvgk iiecmseigg dhdaqdqqnf ihrdtkstie fiindvpyti ngdvpprtsl
       61 nvfirdyaql rgtkamcheg gcgacivsid vkgktmsvns clvpvlicng wtiktieglg
      121 gkkngyhtlq aslagkngsq cgycssgmvm nmysllqgkk ltmkeiensf gsnicrctgy
      181 rpildafkay aadapkdlvq dihdieelfk iksckrtglp cendcnscyt liqntedkid
      241 mklegmefyk vlsvddlfev fakkpdasyi lhggntahgv yrvkmpemai dindipdlqr
      301 iskdndslli ggnlsltvvm etfqqyskep kfeylqhlak hidliasvpv rnigsvagnl
      361 mtkhqhnefp sdlflileta gaqlhiveag gkksivnlld flkmdmkhki iysivlparg
      421 neyeyrsyki mpraqnahah vnagflfkld gagkvlekpn iiigairpdf lhaskteefl
      481 tgksiydknv vnkaietlds eihpdhvlpd yspkfrklla eglffkfils ikpekvdpkm
      541 rsggsvlqrg lssgkqdfdt dknlwplnqp lpkmesihqt sgeaqyvndv pslpkevfca
      601 fvlttvpngn iesidaseal kmkgvvafft akdipgknvf isaasqqiml pndevlfadk
      661 kvefagqpvg vivatthata neaaqkvhvs yvdvqadkal lsiedvlask ddsrllqtan
      721 vkaetkgtdv khvikgvfrc ggqyhytmep qtcvcipied gidvfpasqw vdltqtsiae
      781 cldvknnsln invrrlggay gakisratqi scacalvcyk lnrparfvms iesnmmsigk
      841 ridtrqeyei gvddngriqy lesnfwansg cnfnemhaga iaehvkscyd sstwtyngye
      901 aktdlpsnty crapsstegv aiiensmehi akilgkdplq vklanmnedh kkilqpmied
      961 liknadyemr krdveafnne nrwkkkgial vpmmypmavw gqfhalvsiy ardgtvsvth
     1021 ggiecgqgin tkvaqvaaht lgidlslvtv kptnnltspn nfvtggslas evnayaamva
     1081 ckellkrlep ikqelknptw qelvmtawak dvdlcarymf ttkddikpya iygvtiaeve
     1141 idvltgqhiv qrvdlmedag rsmnpeldlg qiegafvmgl gfwtsedliy dpktgqltny
     1201 rtwnykppga kdipvdfrvy frrnapnpls vlrskatgep plcmscvipi airnalnsar
     1261 kdagdsnpwf qldgpvttek illtsltckk qmvf
//
LOCUS       XP_034185074            1270 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606576 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185074
VERSION     XP_034185074.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329183.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185074.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="uncharacterized protein LOC117606576 isoform X2"
                     /calculated_mol_wt=140622
     Region          39..1257
                     /region_name="PLN02906"
                     /note="xanthine dehydrogenase"
                     /db_xref="CDD:215491"
     CDS             1..1270
                     /gene="LOC117606576"
                     /coded_by="XM_034329183.2:173..3985"
                     /db_xref="GeneID:117606576"
ORIGIN      
        1 mecikrsvgd tkstiefiin dvpytingdv pprtslnvfi rdyaqlrgtk amcheggcga
       61 civsidvkgk tmsvnsclvp vlicngwtik tieglggkkn gyhtlqasla gkngsqcgyc
      121 ssgmvmnmys llqgkkltmk eiensfgsni crctgyrpil dafkayaada pkdlvqdihd
      181 ieelfkiksc krtglpcend cnscytliqn tedkidmkle gmefykvlsv ddlfevfakk
      241 pdasyilhgg ntahgvyrvk mpemaidind ipdlqriskd ndslliggnl sltvvmetfq
      301 qyskepkfey lqhlakhidl iasvpvrnig svagnlmtkh qhnefpsdlf liletagaql
      361 hiveaggkks ivnlldflkm dmkhkiiysi vlpargneye yrsykimpra qnahahvnag
      421 flfkldgagk vlekpniiig airpdflhas kteefltgks iydknvvnka ietldseihp
      481 dhvlpdyspk frkllaeglf fkfilsikpe kvdpkmrsgg svlqrglssg kqdfdtdknl
      541 wplnqplpkm esihqtsgea qyvndvpslp kevfcafvlt tvpngniesi dasealkmkg
      601 vvafftakdi pgknvfisaa sqqimlpnde vlfadkkvef agqpvgviva tthataneaa
      661 qkvhvsyvdv qadkallsie dvlaskddsr llqtanvkae tkgtdvkhvi kgvfrcggqy
      721 hytmepqtcv cipiedgidv fpasqwvdlt qtsiaecldv knnslninvr rlggaygaki
      781 sratqiscac alvcyklnrp arfvmsiesn mmsigkridt rqeyeigvdd ngriqylesn
      841 fwansgcnfn emhagaiaeh vkscydsstw tyngyeaktd lpsntycrap sstegvaiie
      901 nsmehiakil gkdplqvkla nmnedhkkil qpmiedlikn adyemrkrdv eafnnenrwk
      961 kkgialvpmm ypmavwgqfh alvsiyardg tvsvthggie cgqgintkva qvaahtlgid
     1021 lslvtvkptn nltspnnfvt ggslasevna yaamvackel lkrlepikqe lknptwqelv
     1081 mtawakdvdl carymfttkd dikpyaiygv tiaeveidvl tgqhivqrvd lmedagrsmn
     1141 peldlgqieg afvmglgfwt sedliydpkt gqltnyrtwn ykppgakdip vdfrvyfrrn
     1201 apnplsvlrs katgepplcm scvipiairn alnsarkdag dsnpwfqldg pvttekillt
     1261 sltckkqmvf
//
LOCUS       XP_034185075            1262 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606576 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185075
VERSION     XP_034185075.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329184.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185075.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1262
                     /product="uncharacterized protein LOC117606576 isoform X3"
                     /calculated_mol_wt=139749
     Region          31..1249
                     /region_name="PLN02906"
                     /note="xanthine dehydrogenase"
                     /db_xref="CDD:215491"
     CDS             1..1262
                     /gene="LOC117606576"
                     /coded_by="XM_034329184.2:281..4069"
                     /db_xref="GeneID:117606576"
ORIGIN      
        1 mdtkstiefi indvpyting dvpprtslnv firdyaqlrg tkamcheggc gacivsidvk
       61 gktmsvnscl vpvlicngwt iktieglggk kngyhtlqas lagkngsqcg ycssgmvmnm
      121 ysllqgkklt mkeiensfgs nicrctgyrp ildafkayaa dapkdlvqdi hdieelfkik
      181 sckrtglpce ndcnscytli qntedkidmk legmefykvl svddlfevfa kkpdasyilh
      241 ggntahgvyr vkmpemaidi ndipdlqris kdndslligg nlsltvvmet fqqyskepkf
      301 eylqhlakhi dliasvpvrn igsvagnlmt khqhnefpsd lfliletaga qlhiveaggk
      361 ksivnlldfl kmdmkhkiiy sivlpargne yeyrsykimp raqnahahvn agflfkldga
      421 gkvlekpnii igairpdflh askteefltg ksiydknvvn kaietldsei hpdhvlpdys
      481 pkfrkllaeg lffkfilsik pekvdpkmrs ggsvlqrgls sgkqdfdtdk nlwplnqplp
      541 kmesihqtsg eaqyvndvps lpkevfcafv lttvpngnie sidasealkm kgvvafftak
      601 dipgknvfis aasqqimlpn devlfadkkv efagqpvgvi vatthatane aaqkvhvsyv
      661 dvqadkalls iedvlaskdd srllqtanvk aetkgtdvkh vikgvfrcgg qyhytmepqt
      721 cvcipiedgi dvfpasqwvd ltqtsiaecl dvknnslnin vrrlggayga kisratqisc
      781 acalvcykln rparfvmsie snmmsigkri dtrqeyeigv ddngriqyle snfwansgcn
      841 fnemhagaia ehvkscydss twtyngyeak tdlpsntycr apsstegvai iensmehiak
      901 ilgkdplqvk lanmnedhkk ilqpmiedli knadyemrkr dveafnnenr wkkkgialvp
      961 mmypmavwgq fhalvsiyar dgtvsvthgg iecgqgintk vaqvaahtlg idlslvtvkp
     1021 tnnltspnnf vtggslasev nayaamvack ellkrlepik qelknptwqe lvmtawakdv
     1081 dlcarymftt kddikpyaiy gvtiaeveid vltgqhivqr vdlmedagrs mnpeldlgqi
     1141 egafvmglgf wtsedliydp ktgqltnyrt wnykppgakd ipvdfrvyfr rnapnplsvl
     1201 rskatgeppl cmscvipiai rnalnsarkd agdsnpwfql dgpvttekil ltsltckkqm
     1261 vf
//
LOCUS       XP_034185076            1262 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606576 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185076
VERSION     XP_034185076.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329185.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185076.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1262
                     /product="uncharacterized protein LOC117606576 isoform X4"
                     /calculated_mol_wt=139749
     Region          31..1249
                     /region_name="PLN02906"
                     /note="xanthine dehydrogenase"
                     /db_xref="CDD:215491"
     CDS             1..1262
                     /gene="LOC117606576"
                     /coded_by="XM_034329185.2:433..4221"
                     /db_xref="GeneID:117606576"
ORIGIN      
        1 mdtkstiefi indvpyting dvpprtslnv firdyaqlrg tkamcheggc gacivsidvk
       61 gktmsvnscl vpvlicngwt iktieglggk kngyhtlqas lagkngsqcg ycssgmvmnm
      121 ysllqgkklt mkeiensfgs nicrctgyrp ildafkayaa dapkdlvqdi hdieelfkik
      181 sckrtglpce ndcnscytli qntedkidmk legmefykvl svddlfevfa kkpdasyilh
      241 ggntahgvyr vkmpemaidi ndipdlqris kdndslligg nlsltvvmet fqqyskepkf
      301 eylqhlakhi dliasvpvrn igsvagnlmt khqhnefpsd lfliletaga qlhiveaggk
      361 ksivnlldfl kmdmkhkiiy sivlpargne yeyrsykimp raqnahahvn agflfkldga
      421 gkvlekpnii igairpdflh askteefltg ksiydknvvn kaietldsei hpdhvlpdys
      481 pkfrkllaeg lffkfilsik pekvdpkmrs ggsvlqrgls sgkqdfdtdk nlwplnqplp
      541 kmesihqtsg eaqyvndvps lpkevfcafv lttvpngnie sidasealkm kgvvafftak
      601 dipgknvfis aasqqimlpn devlfadkkv efagqpvgvi vatthatane aaqkvhvsyv
      661 dvqadkalls iedvlaskdd srllqtanvk aetkgtdvkh vikgvfrcgg qyhytmepqt
      721 cvcipiedgi dvfpasqwvd ltqtsiaecl dvknnslnin vrrlggayga kisratqisc
      781 acalvcykln rparfvmsie snmmsigkri dtrqeyeigv ddngriqyle snfwansgcn
      841 fnemhagaia ehvkscydss twtyngyeak tdlpsntycr apsstegvai iensmehiak
      901 ilgkdplqvk lanmnedhkk ilqpmiedli knadyemrkr dveafnnenr wkkkgialvp
      961 mmypmavwgq fhalvsiyar dgtvsvthgg iecgqgintk vaqvaahtlg idlslvtvkp
     1021 tnnltspnnf vtggslasev nayaamvack ellkrlepik qelknptwqe lvmtawakdv
     1081 dlcarymftt kddikpyaiy gvtiaeveid vltgqhivqr vdlmedagrs mnpeldlgqi
     1141 egafvmglgf wtsedliydp ktgqltnyrt wnykppgakd ipvdfrvyfr rnapnplsvl
     1201 rskatgeppl cmscvipiai rnalnsarkd agdsnpwfql dgpvttekil ltsltckkqm
     1261 vf
//
LOCUS       XP_034185078            1262 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606576 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185078
VERSION     XP_034185078.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329187.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185078.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1262
                     /product="uncharacterized protein LOC117606576 isoform X5"
                     /calculated_mol_wt=139749
     Region          31..1249
                     /region_name="PLN02906"
                     /note="xanthine dehydrogenase"
                     /db_xref="CDD:215491"
     CDS             1..1262
                     /gene="LOC117606576"
                     /coded_by="XM_034329187.2:537..4325"
                     /db_xref="GeneID:117606576"
ORIGIN      
        1 mdtkstiefi indvpyting dvpprtslnv firdyaqlrg tkamcheggc gacivsidvk
       61 gktmsvnscl vpvlicngwt iktieglggk kngyhtlqas lagkngsqcg ycssgmvmnm
      121 ysllqgkklt mkeiensfgs nicrctgyrp ildafkayaa dapkdlvqdi hdieelfkik
      181 sckrtglpce ndcnscytli qntedkidmk legmefykvl svddlfevfa kkpdasyilh
      241 ggntahgvyr vkmpemaidi ndipdlqris kdndslligg nlsltvvmet fqqyskepkf
      301 eylqhlakhi dliasvpvrn igsvagnlmt khqhnefpsd lfliletaga qlhiveaggk
      361 ksivnlldfl kmdmkhkiiy sivlpargne yeyrsykimp raqnahahvn agflfkldga
      421 gkvlekpnii igairpdflh askteefltg ksiydknvvn kaietldsei hpdhvlpdys
      481 pkfrkllaeg lffkfilsik pekvdpkmrs ggsvlqrgls sgkqdfdtdk nlwplnqplp
      541 kmesihqtsg eaqyvndvps lpkevfcafv lttvpngnie sidasealkm kgvvafftak
      601 dipgknvfis aasqqimlpn devlfadkkv efagqpvgvi vatthatane aaqkvhvsyv
      661 dvqadkalls iedvlaskdd srllqtanvk aetkgtdvkh vikgvfrcgg qyhytmepqt
      721 cvcipiedgi dvfpasqwvd ltqtsiaecl dvknnslnin vrrlggayga kisratqisc
      781 acalvcykln rparfvmsie snmmsigkri dtrqeyeigv ddngriqyle snfwansgcn
      841 fnemhagaia ehvkscydss twtyngyeak tdlpsntycr apsstegvai iensmehiak
      901 ilgkdplqvk lanmnedhkk ilqpmiedli knadyemrkr dveafnnenr wkkkgialvp
      961 mmypmavwgq fhalvsiyar dgtvsvthgg iecgqgintk vaqvaahtlg idlslvtvkp
     1021 tnnltspnnf vtggslasev nayaamvack ellkrlepik qelknptwqe lvmtawakdv
     1081 dlcarymftt kddikpyaiy gvtiaeveid vltgqhivqr vdlmedagrs mnpeldlgqi
     1141 egafvmglgf wtsedliydp ktgqltnyrt wnykppgakd ipvdfrvyfr rnapnplsvl
     1201 rskatgeppl cmscvipiai rnalnsarkd agdsnpwfql dgpvttekil ltsltckkqm
     1261 vf
//
LOCUS       XP_034185079            1262 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606576 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185079
VERSION     XP_034185079.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329188.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185079.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1262
                     /product="uncharacterized protein LOC117606576 isoform X6"
                     /calculated_mol_wt=139749
     Region          31..1249
                     /region_name="PLN02906"
                     /note="xanthine dehydrogenase"
                     /db_xref="CDD:215491"
     CDS             1..1262
                     /gene="LOC117606576"
                     /coded_by="XM_034329188.2:23..3811"
                     /db_xref="GeneID:117606576"
ORIGIN      
        1 mdtkstiefi indvpyting dvpprtslnv firdyaqlrg tkamcheggc gacivsidvk
       61 gktmsvnscl vpvlicngwt iktieglggk kngyhtlqas lagkngsqcg ycssgmvmnm
      121 ysllqgkklt mkeiensfgs nicrctgyrp ildafkayaa dapkdlvqdi hdieelfkik
      181 sckrtglpce ndcnscytli qntedkidmk legmefykvl svddlfevfa kkpdasyilh
      241 ggntahgvyr vkmpemaidi ndipdlqris kdndslligg nlsltvvmet fqqyskepkf
      301 eylqhlakhi dliasvpvrn igsvagnlmt khqhnefpsd lfliletaga qlhiveaggk
      361 ksivnlldfl kmdmkhkiiy sivlpargne yeyrsykimp raqnahahvn agflfkldga
      421 gkvlekpnii igairpdflh askteefltg ksiydknvvn kaietldsei hpdhvlpdys
      481 pkfrkllaeg lffkfilsik pekvdpkmrs ggsvlqrgls sgkqdfdtdk nlwplnqplp
      541 kmesihqtsg eaqyvndvps lpkevfcafv lttvpngnie sidasealkm kgvvafftak
      601 dipgknvfis aasqqimlpn devlfadkkv efagqpvgvi vatthatane aaqkvhvsyv
      661 dvqadkalls iedvlaskdd srllqtanvk aetkgtdvkh vikgvfrcgg qyhytmepqt
      721 cvcipiedgi dvfpasqwvd ltqtsiaecl dvknnslnin vrrlggayga kisratqisc
      781 acalvcykln rparfvmsie snmmsigkri dtrqeyeigv ddngriqyle snfwansgcn
      841 fnemhagaia ehvkscydss twtyngyeak tdlpsntycr apsstegvai iensmehiak
      901 ilgkdplqvk lanmnedhkk ilqpmiedli knadyemrkr dveafnnenr wkkkgialvp
      961 mmypmavwgq fhalvsiyar dgtvsvthgg iecgqgintk vaqvaahtlg idlslvtvkp
     1021 tnnltspnnf vtggslasev nayaamvack ellkrlepik qelknptwqe lvmtawakdv
     1081 dlcarymftt kddikpyaiy gvtiaeveid vltgqhivqr vdlmedagrs mnpeldlgqi
     1141 egafvmglgf wtsedliydp ktgqltnyrt wnykppgakd ipvdfrvyfr rnapnplsvl
     1201 rskatgeppl cmscvipiai rnalnsarkd agdsnpwfql dgpvttekil ltsltckkqm
     1261 vf
//
LOCUS       XP_034185080            1218 aa            linear   INV 03-SEP-2025
DEFINITION  putative ATP-dependent RNA helicase kurz isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185080
VERSION     XP_034185080.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329189.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185080.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1218
                     /product="putative ATP-dependent RNA helicase kurz isoform
                     X1"
                     /calculated_mol_wt=138644
     Region          103..>265
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          297..>887
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     Region          985..1075
                     /region_name="OB_NTP_bind"
                     /note="Oligonucleotide/oligosaccharide-binding (OB)-fold;
                     pfam07717"
                     /db_xref="CDD:400182"
     CDS             1..1218
                     /gene="kz"
                     /coded_by="XM_034329189.2:170..3826"
                     /db_xref="GeneID:117606577"
ORIGIN      
        1 mgnkklnwks rniaqveidd sptrkiildi nyreenydac ntlalpsekr ktkskvekvs
       61 ttrllskkrr kqlekvvekk kkklnratll eelakvqapq eelkhyvslt siqtkglkrh
      121 freidlptke ykvnandede ndavevpvns ikgskkkrla ileeqrkeet isdpnvvgfd
      181 eftdsdstsn nsesegddne lecdnnkpes ndtdvqqdeq eknvpkdtts vensqdqten
      241 ikndkdkasi nkenqspdsk stkkdgkvkv svtvekkpav fvtvqrkpei qaarlklpvv
      301 aeeqviveai ndnpvviitg etgsgkttqv pqflyeagya qekligvtep rrvaaismsk
      361 rvaeemnlte kevsylirfe gnvtpetkik fmtdgvllke iqsdflltky sviildeahe
      421 rsvytdilig llsrivplrn krknplklvi msatlrveel idndklfkqk ppvlsvesrq
      481 fpvtihfnrr tntnyvsdaf kkavkihtrl pdggiliflt gqrevnyvvr klrrafpikd
      541 kketkkrrhk ldknsdslek ekdkssteke ekvddsedns dndnfdddfr skeairynkl
      601 rqkkqiqlpn islddysvvp tddthedlle vddeeegqld edededddvl dlntlknaqp
      661 lwvlplysil psheqakvfe pppenyrlcv vatnvaetsl tipdvkyvid cgrcktrlyd
      721 rvtgvstyqv cytskasanq ragragrvgp ghcyrlyssa vfndqfepfs qseiqrkpvd
      781 dlllqmkvmn idkvvnfpfp tppdivqlkt aekrlqilga lqrpsnkegs ysakitplgq
      841 siaafpvapr ygkmlalshq hnllaytvcm vaalsvqevl meafntdgas kkwlqmrrfw
      901 agvgnnlllg dpmvliravg gaeyagikgk llsfceeygl rhkavveirk lrqqltnein
      961 lnipnlnlti dpemkppldt eakllrqivl agmadqvark vmpdevsedq dknkykyayk
     1021 tmdmedpvfm hsscvlrrtc pewvvyqevy etnklymrgv taiepewlpk fapslcrlgd
     1081 pltdpppryd tetgkvmcsi tgtfgkagwk lpvmdiehpl tvegvkwfac fllegkvcpk
     1141 lkqfvpslls spqsinkawa kliprtqemt qhllsrgims rdklfetwkd drsfllssyq
     1201 kwlpesihgh itiiwppv
//
LOCUS       XP_034185081            1212 aa            linear   INV 03-SEP-2025
DEFINITION  putative ATP-dependent RNA helicase kurz isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185081
VERSION     XP_034185081.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329190.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185081.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1212
                     /product="putative ATP-dependent RNA helicase kurz isoform
                     X2"
                     /calculated_mol_wt=137926
     Region          103..>265
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          297..>881
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     Region          979..1069
                     /region_name="OB_NTP_bind"
                     /note="Oligonucleotide/oligosaccharide-binding (OB)-fold;
                     pfam07717"
                     /db_xref="CDD:400182"
     CDS             1..1212
                     /gene="kz"
                     /coded_by="XM_034329190.2:170..3808"
                     /db_xref="GeneID:117606577"
ORIGIN      
        1 mgnkklnwks rniaqveidd sptrkiildi nyreenydac ntlalpsekr ktkskvekvs
       61 ttrllskkrr kqlekvvekk kkklnratll eelakvqapq eelkhyvslt siqtkglkrh
      121 freidlptke ykvnandede ndavevpvns ikgskkkrla ileeqrkeet isdpnvvgfd
      181 eftdsdstsn nsesegddne lecdnnkpes ndtdvqqdeq eknvpkdtts vensqdqten
      241 ikndkdkasi nkenqspdsk stkkdgkvkv svtvekkpav fvtvqrkpei qaarlklpvv
      301 aeeqviveai ndnpvviitg etgsgkttqv pqflyeagya qekligvtep rrvaaismsk
      361 rvaeemnlte kevsylirfe gnvtpetkik fmtdgvllke iqsysviild eahersvytd
      421 iligllsriv plrnkrknpl klvimsatlr veelidndkl fkqkppvlsv esrqfpvtih
      481 fnrrtntnyv sdafkkavki htrlpdggil ifltgqrevn yvvrklrraf pikdkketkk
      541 rrhkldknsd slekekdkss tekeekvdds ednsdndnfd ddfrskeair ynklrqkkqi
      601 qlpnislddy svvptddthe dllevddeee gqldededed ddvldlntlk naqplwvlpl
      661 ysilpsheqa kvfepppeny rlcvvatnva etsltipdvk yvidcgrckt rlydrvtgvs
      721 tyqvcytska sanqragrag rvgpghcyrl yssavfndqf epfsqseiqr kpvddlllqm
      781 kvmnidkvvn fpfptppdiv qlktaekrlq ilgalqrpsn kegsysakit plgqsiaafp
      841 vaprygkmla lshqhnllay tvcmvaalsv qevlmeafnt dgaskkwlqm rrfwagvgnn
      901 lllgdpmvli ravggaeyag ikgkllsfce eyglrhkavv eirklrqqlt neinlnipnl
      961 nltidpemkp pldteakllr qivlagmadq varkvmpdev sedqdknkyk yayktmdmed
     1021 pvfmhsscvl rrtcpewvvy qevyetnkly mrgvtaiepe wlpkfapslc rlgdpltdpp
     1081 prydtetgkv mcsitgtfgk agwklpvmdi ehpltvegvk wfacfllegk vcpklkqfvp
     1141 sllsspqsin kawakliprt qemtqhllsr gimsrdklfe twkddrsfll ssyqkwlpes
     1201 ihghitiiwp pv
//
LOCUS       XP_034184663            1200 aa            linear   INV 03-SEP-2025
DEFINITION  DNA replication helicase/nuclease 2 [Osmia lignaria lignaria].
ACCESSION   XP_034184663
VERSION     XP_034184663.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328772.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184663.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1200
                     /product="DNA replication helicase/nuclease 2"
                     /calculated_mol_wt=136715
     Region          277..509
                     /region_name="DNA2_N-like"
                     /note="Nuclease domain of the nuclease/helicase DNA2 and
                     related nucleases; cd22318"
                     /db_xref="CDD:411722"
     Site            order(277..279,312..313,316..317,320,420..422,424,441,
                     443..445,460)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411722"
     Region          770..966
                     /region_name="DEXXQc_DNA2"
                     /note="DEXXQ-box helicase domain of DNA2; cd18041"
                     /db_xref="CDD:350799"
     Site            order(770..771,774,797..800,832,965..966)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350799"
     Site            order(821..823,881,883,933)
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:350799"
     Region          <834..1179
                     /region_name="DNA2"
                     /note="Superfamily I DNA and/or RNA helicase [Replication,
                     recombination and repair]; COG1112"
                     /db_xref="CDD:440729"
     CDS             1..1200
                     /gene="Dna2"
                     /coded_by="XM_034328772.2:150..3752"
                     /db_xref="GeneID:117606401"
ORIGIN      
        1 mnkkhvviqt pptprrrrrr rqqrisayfk tnanveeevp ctsennvark ctykkrkais
       61 esmessaiqy ktakipvisi dsdsdisnsn ykiastsvkk nitecalken isvdksknvl
      121 nnlnetitkv qnskspsrnv lhekftieec kpnfkgklni lndspviele geikdsdtes
      181 gcstilkdew fhddqlnlnt lqrckvvdic herdalilsv tnsefqtptv ikcleywkyt
      241 kvekdsiiav earkeedwwi vdnefgfiva ypdilisstt ivggmfckrk silnekfrki
      301 eslpcfktdq tamvigsfvh ellqkaiqkd inkfsdikkl ldsmlqsrdt isilyatgit
      361 ldecrkqmlv fipriyqfiq hylkdkrqee inriennfkg qivdildiee niwlpclgik
      421 gkidvtveve enckrkmmpl eiktgkpsfs lehkgqiily immmaltgqd tdtglllylr
      481 enkiqeiksd hrerrdlmql rnvlayyltp kstemfsest adlnwagfdl pepinhpsac
      541 nncmynslcc aylskdsnnk lplkhplnql kekisqvlsp shtnyvlhwi lllemeenaq
      601 atdkshlnlw tlsaeqrenk kicisnlkml ktikesfcky kytfvrnssn aqdresipyt
      661 efseneyvli stnkrinvsa gfivhlsnas vavrvdrdis qyitnelvhi dkyqsstlyc
      721 snlasvggll annnicaklr eividrkpat fdqgvpqsiv sksskilqtl nenqqrvvlk
      781 altatdyili kgmpgtgktq tliafiellh etgrsvlits htnnavdnil lkllakgidf
      841 lrlgssvhes lkayveknai ascttpeeme iaysksivgv tcygayhpml ctrifdvcvv
      901 desaqilqpt vlrplynark fvlvgdpdql ppiiqssqar qrgadeslfs rldsenntvi
      961 lteqyrmnry imtlanklty ndklkagnml iesatftanh nkilmkaepw vqralsqnls
     1021 dsvmllntgp inklkvnykf cdkypesdqv ysnifeaavl rklvqtllqm hvepenigii
     1081 apyrahvsll knviqngiei ntvdqyqgrd keiilyscak slissnnvkq vqdlevlgdh
     1141 rrltvaitra kckliivgdk stllrytvfq klfsliedkn tihlhdnsdg fswkeitsal
//
LOCUS       XP_034185082            1010 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase mind bomb 2 [Osmia lignaria lignaria].
ACCESSION   XP_034185082
VERSION     XP_034185082.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185082.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1010
                     /product="E3 ubiquitin-protein ligase mind bomb 2"
                     /calculated_mol_wt=110740
     Region          5..82
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          93..137
                     /region_name="ZZ"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     dystrophin, CBP/p300 and many other proteins. The ZZ motif
                     coordinates one or two zinc ions and most likely
                     participates in ligand binding or molecular scaffolding.
                     Many proteins containing ZZ motifs have...; cl00295"
                     /db_xref="CDD:412288"
     Site            order(95,98,110,113,119,122,128,132)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239069"
     Site            order(95,98,119,122)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239069"
     Site            order(96,107,109,115,117)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239069"
     Site            order(108,123,134,137)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239069"
     Site            order(110,113,128,132)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239069"
     Region          164..229
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          277..343
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          352..416
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          424..>540
                     /region_name="PTZ00322"
                     /note="6-phosphofructo-2-kinase/fructose-2,
                     6-biphosphatase; Provisional"
                     /db_xref="CDD:140343"
     Region          488..736
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          488..516
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(491..492,495..497,499..500,504,507,516,518,520,
                     524..525,528..530,532..533,537,540,549,551,553,557..558,
                     561..563,565..566,570,573,582)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          518..549
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          551..582
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          618..650
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          652..686
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          688..719
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          966..1003
                     /region_name="RING-HC_MIBs-like"
                     /note="RING finger, HC subclass, found in mind bomb MIB1,
                     MIB2, RGLG1, RGLG2, and similar proteins; cd16520"
                     /db_xref="CDD:438183"
     CDS             1..1010
                     /gene="mib2"
                     /coded_by="XM_034329191.2:227..3259"
                     /db_xref="GeneID:117606578"
ORIGIN      
        1 mlevglrvvr gqdwkwddqd ggeghagtvv eigkpqsfgn sasnpnpsdr tpdktvivqw
       61 dhgsrsnyri gyqgaydllv fdnaatgvkh pniicdgckr hwiigirwkc tqccdydlct
      121 qcymgdvhdl shtferflta tsvgiqltpr egctkiplkg ifigakvvrg pdwewgnqdg
      181 grgktgrvmd irgwdnessr svatvtwskg stnvyrlgyk gcvdlcyvee atagtyykeh
      241 lpllgqpimt vsdngsnaas trsgipssis sprhstfnvg dklkvlvdvn tlkemqeghg
      301 gwnprmaeyi gkigrvhrit dkgdvrvqfe gcnnrwtfhp galtkvtske afslgdivrv
      361 ktdltavkly qrghgewidv mknalgktgk vikiysdgdl rvaldghtwt fnplsvtlvp
      421 pgteavssqe eanrnkdwpa egvdievekl lrdaargeag vsavqeflkk ypgrvdarag
      481 gpgggkktcl qvaahqgqrd lctllldaga slravdedgd tplhyaafgn qpeimellls
      541 rgaainavnn gkcsalhvav nkqhsqcvkv llrhqcdvnl qdaygdtalh daigkdaldi
      601 tdalcscerv dftlrnkrgf nvlhhaalkg nahaterlva rarhlvdvkk edgfaalhla
      661 alnghkdvaa illspnggha kvdlrnnrrq tplhlatsqg hwalvellvh hnadiastda
      721 dgdtvlhiai vkspnqtnlv ptpescrdsp liyaiwqsla rqnvktelsl acflvsvdrs
      781 ctllehvkns knktpldlle adpqiapyad ifrcyqyqsh stqleienap asqpplyqid
      841 ttsqsevvrg srkgipgsgd asecqnclsl lpnsmkdgnr ynpsssvtif ncaqcghgit
      901 kpdaiqdgql atgeisltks dekmkdnald dkkkesdpdk drekdkdler lryletrvad
      961 leeanmcsic merrrnvafl cghgacehca aplktchmcr kvitkkinly
//
LOCUS       XP_034185084             950 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606579 [Osmia lignaria lignaria].
ACCESSION   XP_034185084
VERSION     XP_034185084.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..950
                     /product="uncharacterized protein LOC117606579"
                     /calculated_mol_wt=108516
     Region          <37..>183
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          76..99
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          100..147
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          148..171
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          172..289
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          265..288
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          286..>408
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          289..348
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          290..313
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          314..337
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          338..362
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          363..386
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          387..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          551..609
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          551..574
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          575..598
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          597..657
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          599..622
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          623..647
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          648..738
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <739..>876
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          739..769
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          770..793
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          793..851
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          794..816
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          817..840
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          841..862
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          865..876
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..950
                     /gene="LOC117606579"
                     /coded_by="XM_034329193.2:432..3284"
                     /db_xref="GeneID:117606579"
ORIGIN      
        1 mhqillwalv valigqvhck cslapivdde rsiayacthg dlndldeist eaewieftvs
       61 rfhhipgnaf srfknlrrls fynchvnfid pdafaglnrl ewlifhgtki hvartawfrp
      121 lidlrklild rcglvhiepd vfrllpriet lglrdndldc lpieelsylr alrtvridgn
      181 pwlcecrqrl deyfgsrsiv qevqclremn vcrkyqcmtp igfpvvpssl ttqhlnnfdr
      241 drltnrgnef qtnvftsldr lpdkttwiei sglvidqlpr yaffrfgnsl rslelnncri
      301 gviengafag lhrlqrlslv gnhfstigay wfrdlvnlqq lilerndieq fersslshvg
      361 dslrhldirn nrlrciavee laelkklerl daignpwlcs crnnlqnflt rknigfeina
      421 grcyanendi pdvssdwplq qttegstvik gkvhwtsfed sikrsnittv rptpstppve
      481 ihtvtpqppt ytgacypekt dgqsrsiyac raitsleelg aipptahtir vmlsnikkis
      541 ehsfsrfngy lsrlefrdcg ieeieprafs nlynleylsl rgnqlesina emlqglsnlr
      601 sldlshnniy ritndvfdrl pyltsldise nsmncigvgy manhlhylss lrvssnpwsc
      661 lcgtkiaefl nsrgirydkh slllredcya tqlpettaip pattvsvptr netiegscts
      721 qedatgiryv csggnllllq sippevtvie fhdghlprlp sgvfhrftnl kelvirnsgl
      781 ttveqgafng mnklenltiq dnplemigss wfvlenlerl dlrgnsikyi epgtfrnlnr
      841 ltylnlegnd lkciftsdln dmpnvyivef sgnplkwrcr veleqflear kimfiklenp
      901 cegkklvrhl llqnktdgsf dcpfecstat nighylfavl llpfliifvh
//
LOCUS       XP_076545564             950 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606579 [Osmia lignaria lignaria].
ACCESSION   XP_076545564
VERSION     XP_076545564.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689449.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..950
                     /product="uncharacterized protein LOC117606579"
                     /calculated_mol_wt=108516
     Region          <37..>183
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          76..99
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          100..147
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          148..171
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          172..289
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          265..288
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          286..>408
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          289..348
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          290..313
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          314..337
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          338..362
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          363..386
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          387..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          551..609
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          551..574
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          575..598
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          597..657
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          599..622
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          623..647
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          648..738
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <739..>876
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          739..769
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          770..793
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          793..851
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          794..816
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          817..840
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          841..862
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          865..876
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..950
                     /gene="LOC117606579"
                     /coded_by="XM_076689449.1:529..3381"
                     /db_xref="GeneID:117606579"
ORIGIN      
        1 mhqillwalv valigqvhck cslapivdde rsiayacthg dlndldeist eaewieftvs
       61 rfhhipgnaf srfknlrrls fynchvnfid pdafaglnrl ewlifhgtki hvartawfrp
      121 lidlrklild rcglvhiepd vfrllpriet lglrdndldc lpieelsylr alrtvridgn
      181 pwlcecrqrl deyfgsrsiv qevqclremn vcrkyqcmtp igfpvvpssl ttqhlnnfdr
      241 drltnrgnef qtnvftsldr lpdkttwiei sglvidqlpr yaffrfgnsl rslelnncri
      301 gviengafag lhrlqrlslv gnhfstigay wfrdlvnlqq lilerndieq fersslshvg
      361 dslrhldirn nrlrciavee laelkklerl daignpwlcs crnnlqnflt rknigfeina
      421 grcyanendi pdvssdwplq qttegstvik gkvhwtsfed sikrsnittv rptpstppve
      481 ihtvtpqppt ytgacypekt dgqsrsiyac raitsleelg aipptahtir vmlsnikkis
      541 ehsfsrfngy lsrlefrdcg ieeieprafs nlynleylsl rgnqlesina emlqglsnlr
      601 sldlshnniy ritndvfdrl pyltsldise nsmncigvgy manhlhylss lrvssnpwsc
      661 lcgtkiaefl nsrgirydkh slllredcya tqlpettaip pattvsvptr netiegscts
      721 qedatgiryv csggnllllq sippevtvie fhdghlprlp sgvfhrftnl kelvirnsgl
      781 ttveqgafng mnklenltiq dnplemigss wfvlenlerl dlrgnsikyi epgtfrnlnr
      841 ltylnlegnd lkciftsdln dmpnvyivef sgnplkwrcr veleqflear kimfiklenp
      901 cegkklvrhl llqnktdgsf dcpfecstat nighylfavl llpfliifvh
//
LOCUS       XP_076545565             950 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606579 [Osmia lignaria lignaria].
ACCESSION   XP_076545565
VERSION     XP_076545565.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689450.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..950
                     /product="uncharacterized protein LOC117606579"
                     /calculated_mol_wt=108516
     Region          <37..>183
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          76..99
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          100..147
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          148..171
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          172..289
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          265..288
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          286..>408
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          289..348
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          290..313
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          314..337
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          338..362
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          363..386
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          387..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          551..609
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          551..574
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          575..598
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          597..657
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          599..622
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          623..647
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          648..738
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <739..>876
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          739..769
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          770..793
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          793..851
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          794..816
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          817..840
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          841..862
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          865..876
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..950
                     /gene="LOC117606579"
                     /coded_by="XM_076689450.1:499..3351"
                     /db_xref="GeneID:117606579"
ORIGIN      
        1 mhqillwalv valigqvhck cslapivdde rsiayacthg dlndldeist eaewieftvs
       61 rfhhipgnaf srfknlrrls fynchvnfid pdafaglnrl ewlifhgtki hvartawfrp
      121 lidlrklild rcglvhiepd vfrllpriet lglrdndldc lpieelsylr alrtvridgn
      181 pwlcecrqrl deyfgsrsiv qevqclremn vcrkyqcmtp igfpvvpssl ttqhlnnfdr
      241 drltnrgnef qtnvftsldr lpdkttwiei sglvidqlpr yaffrfgnsl rslelnncri
      301 gviengafag lhrlqrlslv gnhfstigay wfrdlvnlqq lilerndieq fersslshvg
      361 dslrhldirn nrlrciavee laelkklerl daignpwlcs crnnlqnflt rknigfeina
      421 grcyanendi pdvssdwplq qttegstvik gkvhwtsfed sikrsnittv rptpstppve
      481 ihtvtpqppt ytgacypekt dgqsrsiyac raitsleelg aipptahtir vmlsnikkis
      541 ehsfsrfngy lsrlefrdcg ieeieprafs nlynleylsl rgnqlesina emlqglsnlr
      601 sldlshnniy ritndvfdrl pyltsldise nsmncigvgy manhlhylss lrvssnpwsc
      661 lcgtkiaefl nsrgirydkh slllredcya tqlpettaip pattvsvptr netiegscts
      721 qedatgiryv csggnllllq sippevtvie fhdghlprlp sgvfhrftnl kelvirnsgl
      781 ttveqgafng mnklenltiq dnplemigss wfvlenlerl dlrgnsikyi epgtfrnlnr
      841 ltylnlegnd lkciftsdln dmpnvyivef sgnplkwrcr veleqflear kimfiklenp
      901 cegkklvrhl llqnktdgsf dcpfecstat nighylfavl llpfliifvh
//
LOCUS       XP_034185085             950 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606579 [Osmia lignaria lignaria].
ACCESSION   XP_034185085
VERSION     XP_034185085.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329194.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..950
                     /product="uncharacterized protein LOC117606579"
                     /calculated_mol_wt=108516
     Region          <37..>183
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          76..99
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          100..147
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          148..171
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          172..289
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          265..288
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          286..>408
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          289..348
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          290..313
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          314..337
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          338..362
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          363..386
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          387..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          551..609
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          551..574
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          575..598
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          597..657
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          599..622
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          623..647
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          648..738
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <739..>876
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          739..769
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          770..793
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          793..851
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          794..816
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          817..840
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          841..862
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          865..876
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..950
                     /gene="LOC117606579"
                     /coded_by="XM_034329194.2:441..3293"
                     /db_xref="GeneID:117606579"
ORIGIN      
        1 mhqillwalv valigqvhck cslapivdde rsiayacthg dlndldeist eaewieftvs
       61 rfhhipgnaf srfknlrrls fynchvnfid pdafaglnrl ewlifhgtki hvartawfrp
      121 lidlrklild rcglvhiepd vfrllpriet lglrdndldc lpieelsylr alrtvridgn
      181 pwlcecrqrl deyfgsrsiv qevqclremn vcrkyqcmtp igfpvvpssl ttqhlnnfdr
      241 drltnrgnef qtnvftsldr lpdkttwiei sglvidqlpr yaffrfgnsl rslelnncri
      301 gviengafag lhrlqrlslv gnhfstigay wfrdlvnlqq lilerndieq fersslshvg
      361 dslrhldirn nrlrciavee laelkklerl daignpwlcs crnnlqnflt rknigfeina
      421 grcyanendi pdvssdwplq qttegstvik gkvhwtsfed sikrsnittv rptpstppve
      481 ihtvtpqppt ytgacypekt dgqsrsiyac raitsleelg aipptahtir vmlsnikkis
      541 ehsfsrfngy lsrlefrdcg ieeieprafs nlynleylsl rgnqlesina emlqglsnlr
      601 sldlshnniy ritndvfdrl pyltsldise nsmncigvgy manhlhylss lrvssnpwsc
      661 lcgtkiaefl nsrgirydkh slllredcya tqlpettaip pattvsvptr netiegscts
      721 qedatgiryv csggnllllq sippevtvie fhdghlprlp sgvfhrftnl kelvirnsgl
      781 ttveqgafng mnklenltiq dnplemigss wfvlenlerl dlrgnsikyi epgtfrnlnr
      841 ltylnlegnd lkciftsdln dmpnvyivef sgnplkwrcr veleqflear kimfiklenp
      901 cegkklvrhl llqnktdgsf dcpfecstat nighylfavl llpfliifvh
//
LOCUS       XP_076545566             904 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545566
VERSION     XP_076545566.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689451.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..904
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X1"
                     /calculated_mol_wt=94428
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..904
                     /gene="lap"
                     /coded_by="XM_076689451.1:416..3130"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsagap
      481 gaagsvpnpf msdfpslgpq ppqsnaaafg lfeqgaanqg gesqqqsqtg dlfsaggqad
      541 lfgsdsavpk taeggaagda agetvssata asgkstatpp prppppatam ngatprsasp
      601 ttagaaagka psaasintaa aapgksafed lndsirmalg gspsrpapia qqqvptaqqa
      661 qqaqqaqqqp mqqgfgmfdm vgnmpatgqp vmpggpmagy giptqvpagy gspakqpisa
      721 gqpqnaastg kvltgdldss laslaqnlsi nksaqqqvkg mqwnspknaa ktggpaggwt
      781 pqpmaattga gyrpmgqgmt qlptttlgfp phtaplgmqg vpmgmqgmqg mqgmqgmrpm
      841 mgtmpggpgg mmvaggaapm mmpnanpmmg anlqqqqqpq qqqqpqaatq pqnnqvqldp
      901 fgal
//
LOCUS       XP_076545568             902 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545568
VERSION     XP_076545568.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689453.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..902
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X2"
                     /calculated_mol_wt=94257
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..902
                     /gene="lap"
                     /coded_by="XM_076689453.1:416..3124"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngfaav ppannnfvtd nsfssvfgnq dsqsagapga
      481 agsvpnpfms dfpslgpqpp qsnaaafglf eqgaanqgge sqqqsqtgdl fsaggqadlf
      541 gsdsavpkta eggaagdaag etvssataas gkstatpppr ppppatamng atprsasptt
      601 agaaagkaps aasintaaaa pgksafedln dsirmalggs psrpapiaqq qvptaqqaqq
      661 aqqaqqqpmq qgfgmfdmvg nmpatgqpvm pggpmagygi ptqvpagygs pakqpisagq
      721 pqnaastgkv ltgdldssla slaqnlsink saqqqvkgmq wnspknaakt ggpaggwtpq
      781 pmaattgagy rpmgqgmtql ptttlgfpph taplgmqgvp mgmqgmqgmq gmqgmrpmmg
      841 tmpggpggmm vaggaapmmm pnanpmmgan lqqqqqpqqq qqpqaatqpq nnqvqldpfg
      901 al
//
LOCUS       XP_076545569             899 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545569
VERSION     XP_076545569.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689454.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..899
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X3"
                     /calculated_mol_wt=93959
     Region          21..283
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..899
                     /gene="lap"
                     /coded_by="XM_076689454.1:414..3113"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqgydm
      121 spfirryaky lnekalsyrt vafdfckvkr gkedrtlrtm naekllktlp vlqaqldall
      181 efdctandlt ngvinmafml lfrdlirlfa cyndgiinll ekyfdmnkkq crealdlykk
      241 flirmdrvge flkvaenvgi dkgdipdltk apsslldale qhlaslegkk gsaantptqs
      301 asnrtnvksg vsalsststa fgtaasnnrl dhagnghide alrqqalaee eaamnqykak
      361 vqspssgpst npflssptnn anqpivdlfg assataenqp qkasddllql agnpfadmfg
      421 apqpaaaptt qaqnnmwmtn gngfaavppa nnnfvtdnsf ssvfgnqdsq sagapgaags
      481 vpnpfmsdfp slgpqppqsn aaafglfeqg aanqggesqq qsqtgdlfsa ggqadlfgsd
      541 savpktaegg aagdaagetv ssataasgks tatppprppp patamngatp rsaspttaga
      601 aagkapsaas intaaaapgk safedlndsi rmalggspsr papiaqqqvp taqqaqqaqq
      661 aqqqpmqqgf gmfdmvgnmp atgqpvmpgg pmagygiptq vpagygspak qpisagqpqn
      721 aastgkvltg dldsslasla qnlsinksaq qqvkgmqwns pknaaktggp aggwtpqpma
      781 attgagyrpm gqgmtqlptt tlgfpphtap lgmqgvpmgm qgmqgmqgmq gmrpmmgtmp
      841 ggpggmmvag gaapmmmpna npmmganlqq qqqpqqqqqp qaatqpqnnq vqldpfgal
//
LOCUS       XP_076545570             890 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X4 [Osmia lignaria lignaria].
ACCESSION   XP_076545570
VERSION     XP_076545570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689455.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..890
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X4"
                     /calculated_mol_wt=93027
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..890
                     /gene="lap"
                     /coded_by="XM_076689455.1:416..3088"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngndns fssvfgnqds qsagapgaag svpnpfmsdf
      481 pslgpqppqs naaafglfeq gaanqggesq qqsqtgdlfs aggqadlfgs dsavpktaeg
      541 gaagdaaget vssataasgk statppprpp ppatamngat prsaspttag aaagkapsaa
      601 sintaaaapg ksafedlnds irmalggsps rpapiaqqqv ptaqqaqqaq qaqqqpmqqg
      661 fgmfdmvgnm patgqpvmpg gpmagygipt qvpagygspa kqpisagqpq naastgkvlt
      721 gdldsslasl aqnlsinksa qqqvkgmqwn spknaaktgg paggwtpqpm aattgagyrp
      781 mgqgmtqlpt ttlgfpphta plgmqgvpmg mqgmqgmqgm qgmrpmmgtm pggpggmmva
      841 ggaapmmmpn anpmmganlq qqqqpqqqqq pqaatqpqnn qvqldpfgal
//
LOCUS       XP_076545571             888 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X5 [Osmia lignaria lignaria].
ACCESSION   XP_076545571
VERSION     XP_076545571.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689456.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..888
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X5"
                     /calculated_mol_wt=92856
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..888
                     /gene="lap"
                     /coded_by="XM_076689456.1:416..3082"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtndnsfs svfgnqdsqs agapgaagsv pnpfmsdfps
      481 lgpqppqsna aafglfeqga anqggesqqq sqtgdlfsag gqadlfgsds avpktaegga
      541 agdaagetvs sataasgkst atppprpppp atamngatpr saspttagaa agkapsaasi
      601 ntaaaapgks afedlndsir malggspsrp apiaqqqvpt aqqaqqaqqa qqqpmqqgfg
      661 mfdmvgnmpa tgqpvmpggp magygiptqv pagygspakq pisagqpqna astgkvltgd
      721 ldsslaslaq nlsinksaqq qvkgmqwnsp knaaktggpa ggwtpqpmaa ttgagyrpmg
      781 qgmtqlpttt lgfpphtapl gmqgvpmgmq gmqgmqgmqg mrpmmgtmpg gpggmmvagg
      841 aapmmmpnan pmmganlqqq qqpqqqqqpq aatqpqnnqv qldpfgal
//
LOCUS       XP_076545572             888 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X6 [Osmia lignaria lignaria].
ACCESSION   XP_076545572
VERSION     XP_076545572.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689457.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..888
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X6"
                     /calculated_mol_wt=92838
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..888
                     /gene="lap"
                     /coded_by="XM_076689457.1:417..3083"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsastaf gtaasnnrld hagnghidea lrqqalaeee aamnqykakv qspssgpstn
      361 pflssptnna nqpivdlfga ssataenqpq kasddllqla gnpfadmfga pqpaaapttq
      421 aqnnmwmtng ngfaavppan nnfvtdnsfs svfgnqdsqs agapgaagsv pnpfmsdfps
      481 lgpqppqsna aafglfeqga anqggesqqq sqtgdlfsag gqadlfgsds avpktaegga
      541 agdaagetvs sataasgkst atppprpppp atamngatpr saspttagaa agkapsaasi
      601 ntaaaapgks afedlndsir malggspsrp apiaqqqvpt aqqaqqaqqa qqqpmqqgfg
      661 mfdmvgnmpa tgqpvmpggp magygiptqv pagygspakq pisagqpqna astgkvltgd
      721 ldsslaslaq nlsinksaqq qvkgmqwnsp knaaktggpa ggwtpqpmaa ttgagyrpmg
      781 qgmtqlpttt lgfpphtapl gmqgvpmgmq gmqgmqgmqg mrpmmgtmpg gpggmmvagg
      841 aapmmmpnan pmmganlqqq qqpqqqqqpq aatqpqnnqv qldpfgal
//
LOCUS       XP_076545573             883 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X7 [Osmia lignaria lignaria].
ACCESSION   XP_076545573
VERSION     XP_076545573.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689458.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..883
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X7"
                     /calculated_mol_wt=92280
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..883
                     /gene="lap"
                     /coded_by="XM_076689458.1:416..3067"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsagap
      481 gaagsvpnpf msdfpslgpq ppqsnaaafg lfeqgaanqg gesqqqsqtg dlfsaggqad
      541 lfgsdsavpk taeggaagda agetvssata asgkstatpp prppppatam ngatprsasp
      601 ttagaaagka psaasintaa aapgksafed lndsirmalg gspsrpapia qqqvptaqqa
      661 qqaqqaqqqp mqqgfgmfdm vgnmpatgqp vmpggpmagy giptqvpagy gspakqpisa
      721 gqpqnaastg kvltgdldss laslaqnlsi nksaqqqvkg mqwnspknaa ktggpaggwt
      781 pqpmaattga gyrpmgmqgv pmgmqgmqgm qgmqgmrpmm gtmpggpggm mvaggaapmm
      841 mpnanpmmga nlqqqqqpqq qqqpqaatqp qnnqvqldpf gal
//
LOCUS       XP_076545574             873 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X8 [Osmia lignaria lignaria].
ACCESSION   XP_076545574
VERSION     XP_076545574.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689459.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..873
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X8"
                     /calculated_mol_wt=91972
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..873
                     /gene="lap"
                     /coded_by="XM_076689459.1:417..3038"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsagap
      481 gaagsvpnpf msdfpslgpq ppqsnaaafg lfeqgaanqg gesqqqsqtg dlfsaggqad
      541 lfgsdsavpk taeggaagda agetvssata asgkstatpp prppppatam ngatprsasp
      601 ttagaaagka psaasintaa aapgksafed lndsirmalg gspsrpapia qqqvptaqqa
      661 qqaqqaqqqp mqqgfgmfdm vgnmpatgqp vmpggpmagy giptqvpagy gspakqpisa
      721 gqpqnaastg kvltgdldss laslaqnlsi nksaqqqvkg mqwnspknaa ktggpaggwt
      781 pqpmaattga gyrpmlynac masgmlpava gsrndatsyn hlglssphct igiwignert
      841 khqfckyrvi clenvprlfs lfyvirhept rpr
//
LOCUS       XP_076545575             833 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X9 [Osmia lignaria lignaria].
ACCESSION   XP_076545575
VERSION     XP_076545575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689460.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..833
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X9"
                     /calculated_mol_wt=87049
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..833
                     /gene="lap"
                     /coded_by="XM_076689460.1:416..2917"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsagap
      481 gaagsvpnpf msdfpslgpq ppqsnaaafg lfeqgaanqg gesqqqsqtg dlfsaggqad
      541 lfgsdsavpk taeggaagda agetvssata asgkstatpp prppppatam ngatprsasp
      601 ttagaaagka psaasintaa aapgksafed lndsirmalg gspsrpapia qqqvptaqqa
      661 qqaqqaqqqp mqqgfgmfdm vgnmpatgqp vmpggpmagy giptqvpagy gspakqpisa
      721 gqpqnaastg kvltgdldss laslaqnlsi nksaqqqvkg mqwnspknaa ktggpaggwt
      781 pqpmaattga gyrpmgqgmt qlptttlgfp phtaplefgl etsgrstnsv niv
//
LOCUS       XP_076545577             820 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X10 [Osmia lignaria lignaria].
ACCESSION   XP_076545577
VERSION     XP_076545577.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689462.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X10"
                     /calculated_mol_wt=85142
     Region          <1..204
                     /region_name="VHS_ENTH_ANTH"
                     /note="VHS, ENTH and ANTH domain superfamily; cl02544"
                     /db_xref="CDD:470608"
     CDS             1..820
                     /gene="lap"
                     /coded_by="XM_076689462.1:265..2727"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 mlcygnerft qylassnstf qlsnfldksg vqagarigyd mspfirryak ylnekalsyr
       61 tvafdfckvk rgkedrtlrt mnaekllktl pvlqaqldal lefdctandl tngvinmafm
      121 llfrdlirlf acyndgiinl lekyfdmnkk qcrealdlyk kflirmdrvg eflkvaenvg
      181 idkgdipdlt kapsslldal eqhlaslegk kgsaantptq sasnrtnvks gvsalsstst
      241 afgtaasnnr ldhagnghid ealrqqalae eeaamnqyka kvqspssgps tnpflssptn
      301 nanqpivdlf gassataenq pqkasddllq lagnpfadmf gapqpaaapt tqaqnnmwmt
      361 ngngfaavpp annnfvtdns fssvfgnqds qsagapgaag svpnpfmsdf pslgpqppqs
      421 naaafglfeq gaanqggesq qqsqtgdlfs aggqadlfgs dsavpktaeg gaagdaaget
      481 vssataasgk statppprpp ppatamngat prsaspttag aaagkapsaa sintaaaapg
      541 ksafedlnds irmalggsps rpapiaqqqv ptaqqaqqaq qaqqqpmqqg fgmfdmvgnm
      601 patgqpvmpg gpmagygipt qvpagygspa kqpisagqpq naastgkvlt gdldsslasl
      661 aqnlsinksa qqqvkgmqwn spknaaktgg paggwtpqpm aattgagyrp mgqgmtqlpt
      721 ttlgfpphta plgmqgvpmg mqgmqgmqgm qgmrpmmgtm pggpggmmva ggaapmmmpn
      781 anpmmganlq qqqqpqqqqq pqaatqpqnn qvqldpfgal
//
LOCUS       XP_076545578             820 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X10 [Osmia lignaria lignaria].
ACCESSION   XP_076545578
VERSION     XP_076545578.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689463.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X10"
                     /calculated_mol_wt=85142
     Region          <1..204
                     /region_name="VHS_ENTH_ANTH"
                     /note="VHS, ENTH and ANTH domain superfamily; cl02544"
                     /db_xref="CDD:470608"
     CDS             1..820
                     /gene="lap"
                     /coded_by="XM_076689463.1:265..2727"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 mlcygnerft qylassnstf qlsnfldksg vqagarigyd mspfirryak ylnekalsyr
       61 tvafdfckvk rgkedrtlrt mnaekllktl pvlqaqldal lefdctandl tngvinmafm
      121 llfrdlirlf acyndgiinl lekyfdmnkk qcrealdlyk kflirmdrvg eflkvaenvg
      181 idkgdipdlt kapsslldal eqhlaslegk kgsaantptq sasnrtnvks gvsalsstst
      241 afgtaasnnr ldhagnghid ealrqqalae eeaamnqyka kvqspssgps tnpflssptn
      301 nanqpivdlf gassataenq pqkasddllq lagnpfadmf gapqpaaapt tqaqnnmwmt
      361 ngngfaavpp annnfvtdns fssvfgnqds qsagapgaag svpnpfmsdf pslgpqppqs
      421 naaafglfeq gaanqggesq qqsqtgdlfs aggqadlfgs dsavpktaeg gaagdaaget
      481 vssataasgk statppprpp ppatamngat prsaspttag aaagkapsaa sintaaaapg
      541 ksafedlnds irmalggsps rpapiaqqqv ptaqqaqqaq qaqqqpmqqg fgmfdmvgnm
      601 patgqpvmpg gpmagygipt qvpagygspa kqpisagqpq naastgkvlt gdldsslasl
      661 aqnlsinksa qqqvkgmqwn spknaaktgg paggwtpqpm aattgagyrp mgqgmtqlpt
      721 ttlgfpphta plgmqgvpmg mqgmqgmqgm qgmrpmmgtm pggpggmmva ggaapmmmpn
      781 anpmmganlq qqqqpqqqqq pqaatqpqnn qvqldpfgal
//
LOCUS       XP_076545579             820 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X10 [Osmia lignaria lignaria].
ACCESSION   XP_076545579
VERSION     XP_076545579.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689464.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X10"
                     /calculated_mol_wt=85142
     Region          <1..204
                     /region_name="VHS_ENTH_ANTH"
                     /note="VHS, ENTH and ANTH domain superfamily; cl02544"
                     /db_xref="CDD:470608"
     CDS             1..820
                     /gene="lap"
                     /coded_by="XM_076689464.1:258..2720"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 mlcygnerft qylassnstf qlsnfldksg vqagarigyd mspfirryak ylnekalsyr
       61 tvafdfckvk rgkedrtlrt mnaekllktl pvlqaqldal lefdctandl tngvinmafm
      121 llfrdlirlf acyndgiinl lekyfdmnkk qcrealdlyk kflirmdrvg eflkvaenvg
      181 idkgdipdlt kapsslldal eqhlaslegk kgsaantptq sasnrtnvks gvsalsstst
      241 afgtaasnnr ldhagnghid ealrqqalae eeaamnqyka kvqspssgps tnpflssptn
      301 nanqpivdlf gassataenq pqkasddllq lagnpfadmf gapqpaaapt tqaqnnmwmt
      361 ngngfaavpp annnfvtdns fssvfgnqds qsagapgaag svpnpfmsdf pslgpqppqs
      421 naaafglfeq gaanqggesq qqsqtgdlfs aggqadlfgs dsavpktaeg gaagdaaget
      481 vssataasgk statppprpp ppatamngat prsaspttag aaagkapsaa sintaaaapg
      541 ksafedlnds irmalggsps rpapiaqqqv ptaqqaqqaq qaqqqpmqqg fgmfdmvgnm
      601 patgqpvmpg gpmagygipt qvpagygspa kqpisagqpq naastgkvlt gdldsslasl
      661 aqnlsinksa qqqvkgmqwn spknaaktgg paggwtpqpm aattgagyrp mgqgmtqlpt
      721 ttlgfpphta plgmqgvpmg mqgmqgmqgm qgmrpmmgtm pggpggmmva ggaapmmmpn
      781 anpmmganlq qqqqpqqqqq pqaatqpqnn qvqldpfgal
//
LOCUS       XP_076545580             812 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X11 [Osmia lignaria lignaria].
ACCESSION   XP_076545580
VERSION     XP_076545580.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689465.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..812
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X11"
                     /calculated_mol_wt=84901
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..812
                     /gene="lap"
                     /coded_by="XM_076689465.1:416..2854"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsagap
      481 gaagsvpnpf msdfpslgpq ppqsnaaafg lfeqgaanqg gesqqqsqtg dlfsaggqad
      541 lfgsdsavpk taeggaagda agetvssata asgkstatpp prppppatam ngatprsasp
      601 ttagaaagka psaasintaa aapgksafed lndsirmalg gspsrpapia qqqvptaqqa
      661 qqaqqaqqqp mqqgfgmfdm vgnmpatgqp vmpggpmagy giptqvpagy gspakqpisa
      721 gqpqnaastg kvltgdldss laslaqnlsi nksaqqqvkg mqwnspknaa ktggpaggwt
      781 pqpmaattga gyrpmefgle tsgrstnsvn iv
//
LOCUS       XP_034185107             683 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X12 [Osmia lignaria lignaria].
ACCESSION   XP_034185107
VERSION     XP_034185107.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329216.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..683
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X12"
                     /calculated_mol_wt=73001
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..683
                     /gene="lap"
                     /coded_by="XM_034329216.2:409..2460"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsgfdg
      481 sgsilmpisv aghnnisaag qpqnaastgk vltgdldssl aslaqnlsin ksaqqqvkgm
      541 qwnspknaak tggpaggwtp qpmaattgag yrpmgqgmtq lptttlgfpp htaplgmqgv
      601 pmgmqgmqgm qgmqgmrpmm gtmpggpggm mvaggaapmm mpnanpmmga nlqqqqqpqq
      661 qqqpqaatqp qnnqvqldpf gal
//
LOCUS       XP_076545581             681 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X13 [Osmia lignaria lignaria].
ACCESSION   XP_076545581
VERSION     XP_076545581.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689466.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..681
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X13"
                     /calculated_mol_wt=72830
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..681
                     /gene="lap"
                     /coded_by="XM_076689466.1:409..2454"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngfaav ppannnfvtd nsfssvfgnq dsqsgfdgsg
      481 silmpisvag hnnisaagqp qnaastgkvl tgdldsslas laqnlsinks aqqqvkgmqw
      541 nspknaaktg gpaggwtpqp maattgagyr pmgqgmtqlp tttlgfppht aplgmqgvpm
      601 gmqgmqgmqg mqgmrpmmgt mpggpggmmv aggaapmmmp nanpmmganl qqqqqpqqqq
      661 qpqaatqpqn nqvqldpfga l
//
LOCUS       XP_076545582             669 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X14 [Osmia lignaria lignaria].
ACCESSION   XP_076545582
VERSION     XP_076545582.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689467.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..669
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X14"
                     /calculated_mol_wt=71601
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..669
                     /gene="lap"
                     /coded_by="XM_076689467.1:409..2418"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngndns fssvfgnqds qsgfdgsgsi lmpisvaghn
      481 nisaagqpqn aastgkvltg dldsslasla qnlsinksaq qqvkgmqwns pknaaktggp
      541 aggwtpqpma attgagyrpm gqgmtqlptt tlgfpphtap lgmqgvpmgm qgmqgmqgmq
      601 gmrpmmgtmp ggpggmmvag gaapmmmpna npmmganlqq qqqpqqqqqp qaatqpqnnq
      661 vqldpfgal
//
LOCUS       XP_076545583             662 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X15 [Osmia lignaria lignaria].
ACCESSION   XP_076545583
VERSION     XP_076545583.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689468.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..662
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X15"
                     /calculated_mol_wt=70946
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..662
                     /gene="lap"
                     /coded_by="XM_076689468.1:409..2397"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsaagq
      481 pqnaastgkv ltgdldssla slaqnlsink saqqqvkgmq wnspknaakt ggpaggwtpq
      541 pmaattgagy rpmgqgmtql ptttlgfpph taplgmqgvp mgmqgmqgmq gmqgmrpmmg
      601 tmpggpggmm vaggaapmmm pnanpmmgan lqqqqqpqqq qqpqaatqpq nnqvqldpfg
      661 al
//
LOCUS       XP_034185114             661 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X16 [Osmia lignaria lignaria].
ACCESSION   XP_034185114
VERSION     XP_034185114.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329223.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X16"
                     /calculated_mol_wt=70875
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..661
                     /gene="lap"
                     /coded_by="XM_034329223.2:409..2394"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngngfa avppannnfv tdnsfssvfg nqdsqsagqp
      481 qnaastgkvl tgdldsslas laqnlsinks aqqqvkgmqw nspknaaktg gpaggwtpqp
      541 maattgagyr pmgqgmtqlp tttlgfppht aplgmqgvpm gmqgmqgmqg mqgmrpmmgt
      601 mpggpggmmv aggaapmmmp nanpmmganl qqqqqpqqqq qpqaatqpqn nqvqldpfga
      661 l
//
LOCUS       XP_076545584             660 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X17 [Osmia lignaria lignaria].
ACCESSION   XP_076545584
VERSION     XP_076545584.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689469.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..660
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X17"
                     /calculated_mol_wt=70775
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     CDS             1..660
                     /gene="lap"
                     /coded_by="XM_076689469.1:409..2391"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngfaav ppannnfvtd nsfssvfgnq dsqsaagqpq
      481 naastgkvlt gdldsslasl aqnlsinksa qqqvkgmqwn spknaaktgg paggwtpqpm
      541 aattgagyrp mgqgmtqlpt ttlgfpphta plgmqgvpmg mqgmqgmqgm qgmrpmmgtm
      601 pggpggmmva ggaapmmmpn anpmmganlq qqqqpqqqqq pqaatqpqnn qvqldpfgal
//
LOCUS       XP_076545585             659 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X18 [Osmia lignaria lignaria].
ACCESSION   XP_076545585
VERSION     XP_076545585.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689470.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..659
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X18"
                     /calculated_mol_wt=70704
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     Region          367..>546
                     /region_name="PRK11901"
                     /note="hypothetical protein; Reviewed"
                     /db_xref="CDD:237015"
     CDS             1..659
                     /gene="lap"
                     /coded_by="XM_076689470.1:409..2388"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngfaav ppannnfvtd nsfssvfgnq dsqsagqpqn
      481 aastgkvltg dldsslasla qnlsinksaq qqvkgmqwns pknaaktggp aggwtpqpma
      541 attgagyrpm gqgmtqlptt tlgfpphtap lgmqgvpmgm qgmqgmqgmq gmrpmmgtmp
      601 ggpggmmvag gaapmmmpna npmmganlqq qqqpqqqqqp qaatqpqnnq vqldpfgal
//
LOCUS       XP_076545586             648 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X19 [Osmia lignaria lignaria].
ACCESSION   XP_076545586
VERSION     XP_076545586.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689471.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..648
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X19"
                     /calculated_mol_wt=69545
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     Region          <347..>561
                     /region_name="PRK10118"
                     /note="flagellar hook length control protein FliK"
                     /db_xref="CDD:236652"
     CDS             1..648
                     /gene="lap"
                     /coded_by="XM_076689471.1:409..2355"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngndns fssvfgnqds qsaagqpqna astgkvltgd
      481 ldsslaslaq nlsinksaqq qvkgmqwnsp knaaktggpa ggwtpqpmaa ttgagyrpmg
      541 qgmtqlpttt lgfpphtapl gmqgvpmgmq gmqgmqgmqg mrpmmgtmpg gpggmmvagg
      601 aapmmmpnan pmmganlqqq qqpqqqqqpq aatqpqnnqv qldpfgal
//
LOCUS       XP_076545587             647 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X20 [Osmia lignaria lignaria].
ACCESSION   XP_076545587
VERSION     XP_076545587.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689472.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..647
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X20"
                     /calculated_mol_wt=69474
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     Region          <347..>560
                     /region_name="PRK10118"
                     /note="flagellar hook length control protein FliK"
                     /db_xref="CDD:236652"
     CDS             1..647
                     /gene="lap"
                     /coded_by="XM_076689472.1:409..2352"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtngndns fssvfgnqds qsagqpqnaa stgkvltgdl
      481 dsslaslaqn lsinksaqqq vkgmqwnspk naaktggpag gwtpqpmaat tgagyrpmgq
      541 gmtqlptttl gfpphtaplg mqgvpmgmqg mqgmqgmqgm rpmmgtmpgg pggmmvagga
      601 apmmmpnanp mmganlqqqq qpqqqqqpqa atqpqnnqvq ldpfgal
//
LOCUS       XP_076545588             646 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X21 [Osmia lignaria lignaria].
ACCESSION   XP_076545588
VERSION     XP_076545588.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689473.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X21"
                     /calculated_mol_wt=69374
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     Region          <347..>559
                     /region_name="PRK10118"
                     /note="flagellar hook length control protein FliK"
                     /db_xref="CDD:236652"
     CDS             1..646
                     /gene="lap"
                     /coded_by="XM_076689473.1:409..2349"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtndnsfs svfgnqdsqs aagqpqnaas tgkvltgdld
      481 sslaslaqnl sinksaqqqv kgmqwnspkn aaktggpagg wtpqpmaatt gagyrpmgqg
      541 mtqlptttlg fpphtaplgm qgvpmgmqgm qgmqgmqgmr pmmgtmpggp ggmmvaggaa
      601 pmmmpnanpm mganlqqqqq pqqqqqpqaa tqpqnnqvql dpfgal
//
LOCUS       XP_076545589             645 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol-binding clathrin assembly protein lap isoform
            X22 [Osmia lignaria lignaria].
ACCESSION   XP_076545589
VERSION     XP_076545589.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689474.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..645
                     /product="phosphatidylinositol-binding clathrin assembly
                     protein lap isoform X22"
                     /calculated_mol_wt=69303
     Region          21..288
                     /region_name="ANTH"
                     /note="ANTH domain; pfam07651"
                     /db_xref="CDD:400137"
     Site            order(28,38,40..41)
                     /site_type="other"
                     /note="PtdIns(4,5)P2-binding site [chemical binding]"
                     /db_xref="CDD:340782"
     Region          <347..>558
                     /region_name="PRK10118"
                     /note="flagellar hook length control protein FliK"
                     /db_xref="CDD:236652"
     CDS             1..645
                     /gene="lap"
                     /coded_by="XM_076689474.1:409..2346"
                     /db_xref="GeneID:117606580"
ORIGIN      
        1 magqtindrl laarhsiagq glakavckat teemigpkkk hleylvrctn epnvsipqla
       61 nlliersqnt nwtvvfkali tvhhmlcygn erftqylass nstfqlsnfl dksgvqagar
      121 igydmspfir ryakylneka lsyrtvafdf ckvkrgkedr tlrtmnaekl lktlpvlqaq
      181 ldallefdct andltngvin mafmllfrdl irlfacyndg iinllekyfd mnkkqcreal
      241 dlykkflirm drvgeflkva envgidkgdi pdltkapssl ldaleqhlas legkkgsaan
      301 tptqsasnrt nvksgvsals ststafgtaa snnrldhagn ghidealrqq alaeeeaamn
      361 qykakvqsps sgpstnpfls sptnnanqpi vdlfgassat aenqpqkasd dllqlagnpf
      421 admfgapqpa aapttqaqnn mwmtndnsfs svfgnqdsqs agqpqnaast gkvltgdlds
      481 slaslaqnls inksaqqqvk gmqwnspkna aktggpaggw tpqpmaattg agyrpmgqgm
      541 tqlptttlgf pphtaplgmq gvpmgmqgmq gmqgmqgmrp mmgtmpggpg gmmvaggaap
      601 mmmpnanpmm ganlqqqqqp qqqqqpqaat qpqnnqvqld pfgal
//
LOCUS       XP_076545590             900 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic 10-formyltetrahydrofolate dehydrogenase [Osmia lignaria
            lignaria].
ACCESSION   XP_076545590
VERSION     XP_076545590.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689475.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..900
                     /product="cytosolic 10-formyltetrahydrofolate
                     dehydrogenase"
                     /calculated_mol_wt=99397
     Region          4..206
                     /region_name="FMT_core"
                     /note="Formyltransferase, catalytic core domain; cl00395"
                     /db_xref="CDD:444886"
     Site            order(10,13..15,86..93,98,107..110,118,138,140..141,
                     144..145)
                     /site_type="active"
                     /db_xref="CDD:187716"
     Site            order(13..14,87..88,108,110,175)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:187716"
     Site            order(86,89,91..93,98,107,140..141,144..145)
                     /site_type="other"
                     /note="cosubstrate binding site"
                     /db_xref="CDD:187716"
     Site            order(107,109,145)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:187716"
     Region          210..308
                     /region_name="FDH_Hydrolase_C"
                     /note="The C-terminal subdomain of the hydrolase domain on
                     the bi-functional protein 10-formyltetrahydrofolate
                     dehydrogenase; cd08703"
                     /db_xref="CDD:187731"
     Region          327..381
                     /region_name="PP-binding"
                     /note="Phosphopantetheine attachment site; pfam00550"
                     /db_xref="CDD:425746"
     Region          416..900
                     /region_name="ALDH-SF"
                     /note="NAD(P)+-dependent aldehyde dehydrogenase
                     superfamily; cl11961"
                     /db_xref="CDD:448367"
     Site            order(568..572,580,595,597..598,646..649,652,655..656,
                     671..673,705,802,804,832,870)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143395"
     Site            order(572,671,702,705)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143395"
     CDS             1..900
                     /gene="LOC143305501"
                     /coded_by="XM_076689475.1:1..2703"
                     /db_xref="GeneID:143305501"
ORIGIN      
        1 maqlkvaiig qspfaaevyk llkqsghqit gvftipdkgn redplavtak adntpvfkik
       61 swrskgvtlp evlelykrie vdlnvlpfcs qfipmevinh prhrsicyhp sllprhrgas
      121 ainwtliegd ntagfsifwa ddgldtgpil lqrsckvepk dtvdtlynnf lypegikalg
      181 eavdlvakgt apmipqpeeg asydpmlnkk elqkinwskp akqihnfirg mdstpgawtt
      241 indeevrlfg sslwnsdqvp aheiqvnved rvgiiheggl linaiddrfv nverikigtr
      301 tipaskygqk sedtviqfte eelatvdtvr niwagilnmd vendtdffac gagsmdvvrl
      361 veevkdnlgv tlqnidvfmn pvfqqfvttv vlaargisas keikydavel qannmtlkfp
      421 kqlfidgefv nghgkpidti nphdesvicp vecgsmedvd ravkaakkaf eegewskisa
      481 rergsllfkl adlmeqhkee latiesldsg avytlalkth igmsietwry fagwcdkiqg
      541 stipisharp nrnlsftrke pigvcglvtp wnyplmmlsw kmaaclaagn tvvmkpaqas
      601 pltalkfael aalagfppgv inivpgngtv tgnaicehpl irklgftgst qigqsimrsc
      661 ansnlkkvtl elggksplvi fedadvqhav kvgmssvffn kgenciaagr lfveesihde
      721 fvrriveetk kisignpldr rtahgpqnhk ahlnklldfv hrgvkegakl vyggkrldrp
      781 gwyfeptift dvkddmyiak eesfgpvmvi skfnsknmde viaranntey glasgvltkd
      841 isralrfaek ieagtvfint ynktdvaapf ggfkmsgfgk dlgqealney lktktvtiey
//
LOCUS       XP_034185121             739 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606582 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185121
VERSION     XP_034185121.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329230.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..739
                     /product="uncharacterized protein LOC117606582 isoform X1"
                     /calculated_mol_wt=78968
     Region          111..180
                     /region_name="Ubl_midnolin"
                     /note="ubiquitin-like (Ubl) domain found in midnolin and
                     similar proteins; cd01804"
                     /db_xref="CDD:340502"
     CDS             1..739
                     /gene="LOC117606582"
                     /coded_by="XM_034329230.2:110..2329"
                     /db_xref="GeneID:117606582"
ORIGIN      
        1 mdgstsppsd vtamgsenee gsktergtdd tvtsvssnsi nstysitqpd qsttreyrgq
       61 dsiragsasp lpttspssss slssslsppp ppqpqpgcgc vpatvlepde itisitpttg
      121 gqfdltvdrt dtvenlkkii skrlkvaker icllqrerql regtleenrl qdgsrltllp
      181 svetgllaqr peqsvmqale slndsqvndf lsgkaplnlt mrlgdhmmli qlqlstvtpp
      241 spttnqatss ttgltiggns pngsnlstsh vstslqsrrs tvlaarstss ssstklqngt
      301 aasrfsplir laealhaart ncstnlstat tttspvcssr attapsvsst ssysstspph
      361 ssfcanqsqs trttsnhgss sppnttnssa cqscllyhhh llyhhshhhh hhhhhhhhhs
      421 rnksttspnt tqsstsvdhl qqststghgc messmpydvt pprkklctah hhsqqsmsga
      481 dstrstavdg dssvqspass laeqnsktat ldtralveas rnltqklkql ssevltsrvd
      541 laeenarrrq gaiiesmhhh gkgvysgtfs gtlnpalqdr hgrpkrdist iihilndllc
      601 atpsataghy rhhhrhssgr qqssaspsss gtttvngtst sigchdsptp gysseelske
      661 neatkgkmrr lrlvmeqrra krkarkqara apyttqwaav tpdpdpnhdp ntsstatana
      721 tepqnepelq pcspepvva
//
LOCUS       XP_034185122             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606582 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185122
VERSION     XP_034185122.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329231.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC117606582 isoform X2"
                     /calculated_mol_wt=77884
     Region          111..180
                     /region_name="Ubl_midnolin"
                     /note="ubiquitin-like (Ubl) domain found in midnolin and
                     similar proteins; cd01804"
                     /db_xref="CDD:340502"
     CDS             1..729
                     /gene="LOC117606582"
                     /coded_by="XM_034329231.2:110..2299"
                     /db_xref="GeneID:117606582"
ORIGIN      
        1 mdgstsppsd vtamgsenee gsktergtdd tvtsvssnsi nstysitqpd qsttreyrgq
       61 dsiragsasp lpttspssss slssslsppp ppqpqpgcgc vpatvlepde itisitpttg
      121 gqfdltvdrt dtvenlkkii skrlkvaker icllqrerql regtleenrl qdgsrltllp
      181 svetgllaqr peqsvmqale slndsqvndf lsgkaplnlt mrlgdhmmli qlqlstvtpp
      241 spttnqatss ttgltiggns pngsnlstsh vstslqsrrs tvlaarstss ssstklqngt
      301 aasrfsplir laealhaart ncstnlstat tttspvcssr attapsvsst ssysstspph
      361 ssfcanqsqs trttsnhgss sppnttnssa cqscllyhhh llyhhshhhh hhhhhhhhhs
      421 rnksttspnt tqsstsvdhl qqststghgc messmpydvt pprkklctah hhsqqsmsga
      481 dstrstavdg dssvqspass laeqnsktat ldtralveas rnltqklkql sseenarrrq
      541 gaiiesmhhh gkgvysgtfs gtlnpalqdr hgrpkrdist iihilndllc atpsataghy
      601 rhhhrhssgr qqssaspsss gtttvngtst sigchdsptp gysseelske neatkgkmrr
      661 lrlvmeqrra krkarkqara apyttqwaav tpdpdpnhdp ntsstatana tepqnepelq
      721 pcspepvva
//
LOCUS       XP_034185123             665 aa            linear   INV 03-SEP-2025
DEFINITION  intraflagellar transport protein 81 homolog isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185123
VERSION     XP_034185123.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329232.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185123.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..665
                     /product="intraflagellar transport protein 81 homolog
                     isoform X1"
                     /calculated_mol_wt=76860
     Region          3..124
                     /region_name="IFT81_CH"
                     /note="Intraflagellar transport 81 calponin homology
                     domain; pfam18383"
                     /db_xref="CDD:465736"
     Region          <134..>553
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     CDS             1..665
                     /gene="LOC117606584"
                     /coded_by="XM_034329232.2:424..2421"
                     /db_xref="GeneID:117606584"
ORIGIN      
        1 mkentkllvt elnkllnrny nviyfnslss eellqvlkev likiqdqnda nvdtrngste
       61 etsiyilsvl rvlnyqpqvd patfrqglir gdpdmihpil twllsnldiv rkraylsrfl
      121 vkievpaeyl sdaevsslye qytasinrfk tvhkereigk knfenaselt adlktmekek
      181 eavtiriekm kakaepgihf ldaaralrie kdkerdlalq eeqekeilst lqtslqrler
      241 elqilkkden evtvksllqq lseaitvqti vtnekipaei ktkkdrikal vavkqysyls
      301 pdqitalrnk ldtiseeiqk miehkisksn idkiepfrqq aaavtnikrn alerlekter
      361 slqelqakle ekqelskfmv edivpkgeel kkyvnrlktr gtlykhckae lawlaaessv
      421 lyrttailey qvnqcnqake ilenvqktip dnfteenals mnvklfrdis afrgklvpfi
      481 ndvqtlrkks hqyqqqqeqv kkthnnvess mnvsindlqs eieniklkmt kdveekeklq
      541 klikrmknte erinrdaeds kisdpgkklk eelnsaivse eakiksllte kdrlkeivav
      601 kekqtqmwsd ilsyvvdiis iekdicitnk ycfrifqcki ksaeetkqsn givvrrggae
      661 tlvlq
//
LOCUS       XP_034185124             644 aa            linear   INV 03-SEP-2025
DEFINITION  intraflagellar transport protein 81 homolog isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185124
VERSION     XP_034185124.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329233.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185124.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..644
                     /product="intraflagellar transport protein 81 homolog
                     isoform X2"
                     /calculated_mol_wt=74342
     Region          3..124
                     /region_name="IFT81_CH"
                     /note="Intraflagellar transport 81 calponin homology
                     domain; pfam18383"
                     /db_xref="CDD:465736"
     Region          <134..>553
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     CDS             1..644
                     /gene="LOC117606584"
                     /coded_by="XM_034329233.2:424..2358"
                     /db_xref="GeneID:117606584"
ORIGIN      
        1 mkentkllvt elnkllnrny nviyfnslss eellqvlkev likiqdqnda nvdtrngste
       61 etsiyilsvl rvlnyqpqvd patfrqglir gdpdmihpil twllsnldiv rkraylsrfl
      121 vkievpaeyl sdaevsslye qytasinrfk tvhkereigk knfenaselt adlktmekek
      181 eavtiriekm kakaepgihf ldaaralrie kdkerdlalq eeqekeilst lqtslqrler
      241 elqilkkden evtvksllqq lseaitvqti vtnekipaei ktkkdrikal vavkqysyls
      301 pdqitalrnk ldtiseeiqk miehkisksn idkiepfrqq aaavtnikrn alerlekter
      361 slqelqakle ekqelskfmv edivpkgeel kkyvnrlktr gtlykhckae lawlaaessv
      421 lyrttailey qvnqcnqake ilenvqktip dnfteenals mnvklfrdis afrgklvpfi
      481 ndvqtlrkks hqyqqqqeqv kkthnnvess mnvsindlqs eieniklkmt kdveekeklq
      541 klikrmknte erinrdaeds kisdpgkklk eelnsaivse eakiksllte kdrlkeivav
      601 kekqtqmwsd ilsifqckik saeetkqsng ivvrrggaet lvlq
//
LOCUS       XP_034185125             664 aa            linear   INV 03-SEP-2025
DEFINITION  ATP binding cassette subfamily D member Pmp70 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185125
VERSION     XP_034185125.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329234.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..664
                     /product="ATP binding cassette subfamily D member Pmp70"
                     /calculated_mol_wt=75714
     Region          53..652
                     /region_name="3a01203"
                     /note="Peroxysomal Fatty Acyl CoA Transporter (FAT) Family
                     protein; TIGR00954"
                     /db_xref="CDD:273360"
     CDS             1..664
                     /gene="Pmp70"
                     /coded_by="XM_034329234.2:446..2440"
                     /db_xref="GeneID:117606585"
ORIGIN      
        1 mapslskfas rrtvagacal aaliwilkkr gkkqvqkcrn vwpandiqyv ikeekpknpk
       61 ayvnarffrq lrkllkigip svastefgfv lviaaslvar sicdlwmini gtliessivn
      121 mdvsifkrrl fkfltllpli sivnnvlkyg ifemklrlrt nitrnlmdqy lkgftyykms
      181 nldsrianpd qllttdvdkf cesctdlysn vakplldivi yvyrlttnlg gqtplimlsy
      241 lvfagtilth lrrpigqmtv keqrlegeyr hinsrlitns eeiafyqgnn rekltlltsf
      301 hklvthlrkc lefktligii dnfvgkyvat ivgfyavsip ffqknhpvls gtadhrfkny
      361 ytygrmlvkl aeaigrlvla greltklagy tarvteikev lddlnagkye rtmisdfkde
      421 pigspgagki vtkdnvirfd cvplitpngd ilvrelsfev ksginvlvcg pngcgksslf
      481 rilgelwpvw sgtvtkpprg klfyipqrpy mtlgtlrdqv iyphtkqemm rrgqmtdedl
      541 kkllnlvqls hllerenfts segqgwdvva dwmdvlsgge kqriamarlf yhkpqfaild
      601 ectsavsmdv edsmysycrq anitlftvsh rrslwkhhey ylqmdgrgny efkpiesdte
      661 efgs
//
LOCUS       XP_034185126             664 aa            linear   INV 03-SEP-2025
DEFINITION  ATP binding cassette subfamily D member Pmp70 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185126
VERSION     XP_034185126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..664
                     /product="ATP binding cassette subfamily D member Pmp70"
                     /calculated_mol_wt=75714
     Region          53..652
                     /region_name="3a01203"
                     /note="Peroxysomal Fatty Acyl CoA Transporter (FAT) Family
                     protein; TIGR00954"
                     /db_xref="CDD:273360"
     CDS             1..664
                     /gene="Pmp70"
                     /coded_by="XM_034329235.2:422..2416"
                     /db_xref="GeneID:117606585"
ORIGIN      
        1 mapslskfas rrtvagacal aaliwilkkr gkkqvqkcrn vwpandiqyv ikeekpknpk
       61 ayvnarffrq lrkllkigip svastefgfv lviaaslvar sicdlwmini gtliessivn
      121 mdvsifkrrl fkfltllpli sivnnvlkyg ifemklrlrt nitrnlmdqy lkgftyykms
      181 nldsrianpd qllttdvdkf cesctdlysn vakplldivi yvyrlttnlg gqtplimlsy
      241 lvfagtilth lrrpigqmtv keqrlegeyr hinsrlitns eeiafyqgnn rekltlltsf
      301 hklvthlrkc lefktligii dnfvgkyvat ivgfyavsip ffqknhpvls gtadhrfkny
      361 ytygrmlvkl aeaigrlvla greltklagy tarvteikev lddlnagkye rtmisdfkde
      421 pigspgagki vtkdnvirfd cvplitpngd ilvrelsfev ksginvlvcg pngcgksslf
      481 rilgelwpvw sgtvtkpprg klfyipqrpy mtlgtlrdqv iyphtkqemm rrgqmtdedl
      541 kkllnlvqls hllerenfts segqgwdvva dwmdvlsgge kqriamarlf yhkpqfaild
      601 ectsavsmdv edsmysycrq anitlftvsh rrslwkhhey ylqmdgrgny efkpiesdte
      661 efgs
//
LOCUS       XP_034185128             624 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606586 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185128
VERSION     XP_034185128.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329237.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185128.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="uncharacterized protein LOC117606586 isoform X1"
                     /calculated_mol_wt=72024
     Region          <71..347
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          116..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          141..161
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          163..185
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          204..238
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          239..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..294
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          295..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..349
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..624
                     /gene="LOC117606586"
                     /coded_by="XM_034329237.2:197..2071"
                     /db_xref="GeneID:117606586"
ORIGIN      
        1 mkfnkyqyss cidpnrtnss lkehpdmkls cyyqlpnqnf fkcfctsics atkrldmkgs
       61 visekirrhf qdtdipalli flkkhpdiih inlanndisd tgfmnlldhv llyksvenld
      121 lqnnnitekg tnyllaraey leikylnlka nkfgvqaskn vatyllknkh ivalnvaevd
      181 qsassliyfv mglsseqeis nntlrdldis rpnpgfmyyf dsvhfadvig hmlknntclr
      241 alhlqkynfs chdienmmsn akynntlyll dlgnnnigdh gvehitswli krpalktlil
      301 crniitdhga rslsftlpfs kilvldisyn kmtdngivdi lntlkkspll rqlrifgnci
      361 ghpaakiikr mlmfqvleqe nidvrpyrvd hkwyfaryeg srckkeyhdi pyglhfnasr
      421 pptkinrpnm kyykytytkt telkvqhsii tiisrilsrq hvqdckccyc ikceaphfde
      481 lcrdanhped ctcckckads eisidksiid rlvpvidvmk sisyilkrvn cevqdkivrw
      541 vninedilee dletiacnre dvktssedtv lcntswvqln mptlqkylqq tssknvlivp
      601 knylvysnsa demsslqycp nksm
//
LOCUS       XP_034185129             624 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606586 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185129
VERSION     XP_034185129.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329238.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185129.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="uncharacterized protein LOC117606586 isoform X1"
                     /calculated_mol_wt=72024
     Region          <71..347
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          116..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          141..161
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          163..185
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          204..238
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          239..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..294
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          295..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..349
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..624
                     /gene="LOC117606586"
                     /coded_by="XM_034329238.2:199..2073"
                     /db_xref="GeneID:117606586"
ORIGIN      
        1 mkfnkyqyss cidpnrtnss lkehpdmkls cyyqlpnqnf fkcfctsics atkrldmkgs
       61 visekirrhf qdtdipalli flkkhpdiih inlanndisd tgfmnlldhv llyksvenld
      121 lqnnnitekg tnyllaraey leikylnlka nkfgvqaskn vatyllknkh ivalnvaevd
      181 qsassliyfv mglsseqeis nntlrdldis rpnpgfmyyf dsvhfadvig hmlknntclr
      241 alhlqkynfs chdienmmsn akynntlyll dlgnnnigdh gvehitswli krpalktlil
      301 crniitdhga rslsftlpfs kilvldisyn kmtdngivdi lntlkkspll rqlrifgnci
      361 ghpaakiikr mlmfqvleqe nidvrpyrvd hkwyfaryeg srckkeyhdi pyglhfnasr
      421 pptkinrpnm kyykytytkt telkvqhsii tiisrilsrq hvqdckccyc ikceaphfde
      481 lcrdanhped ctcckckads eisidksiid rlvpvidvmk sisyilkrvn cevqdkivrw
      541 vninedilee dletiacnre dvktssedtv lcntswvqln mptlqkylqq tssknvlivp
      601 knylvysnsa demsslqycp nksm
//
LOCUS       XP_034185131             624 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606586 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185131
VERSION     XP_034185131.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329240.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185131.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="uncharacterized protein LOC117606586 isoform X1"
                     /calculated_mol_wt=72024
     Region          <71..347
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          116..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          141..161
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          163..185
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          204..238
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          239..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..294
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          295..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..349
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..624
                     /gene="LOC117606586"
                     /coded_by="XM_034329240.2:178..2052"
                     /db_xref="GeneID:117606586"
ORIGIN      
        1 mkfnkyqyss cidpnrtnss lkehpdmkls cyyqlpnqnf fkcfctsics atkrldmkgs
       61 visekirrhf qdtdipalli flkkhpdiih inlanndisd tgfmnlldhv llyksvenld
      121 lqnnnitekg tnyllaraey leikylnlka nkfgvqaskn vatyllknkh ivalnvaevd
      181 qsassliyfv mglsseqeis nntlrdldis rpnpgfmyyf dsvhfadvig hmlknntclr
      241 alhlqkynfs chdienmmsn akynntlyll dlgnnnigdh gvehitswli krpalktlil
      301 crniitdhga rslsftlpfs kilvldisyn kmtdngivdi lntlkkspll rqlrifgnci
      361 ghpaakiikr mlmfqvleqe nidvrpyrvd hkwyfaryeg srckkeyhdi pyglhfnasr
      421 pptkinrpnm kyykytytkt telkvqhsii tiisrilsrq hvqdckccyc ikceaphfde
      481 lcrdanhped ctcckckads eisidksiid rlvpvidvmk sisyilkrvn cevqdkivrw
      541 vninedilee dletiacnre dvktssedtv lcntswvqln mptlqkylqq tssknvlivp
      601 knylvysnsa demsslqycp nksm
//
LOCUS       XP_034185133             624 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606586 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185133
VERSION     XP_034185133.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329242.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185133.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="uncharacterized protein LOC117606586 isoform X1"
                     /calculated_mol_wt=72024
     Region          <71..347
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          116..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          141..161
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          163..185
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          204..238
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          239..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..294
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          295..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..349
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..624
                     /gene="LOC117606586"
                     /coded_by="XM_034329242.2:197..2071"
                     /db_xref="GeneID:117606586"
ORIGIN      
        1 mkfnkyqyss cidpnrtnss lkehpdmkls cyyqlpnqnf fkcfctsics atkrldmkgs
       61 visekirrhf qdtdipalli flkkhpdiih inlanndisd tgfmnlldhv llyksvenld
      121 lqnnnitekg tnyllaraey leikylnlka nkfgvqaskn vatyllknkh ivalnvaevd
      181 qsassliyfv mglsseqeis nntlrdldis rpnpgfmyyf dsvhfadvig hmlknntclr
      241 alhlqkynfs chdienmmsn akynntlyll dlgnnnigdh gvehitswli krpalktlil
      301 crniitdhga rslsftlpfs kilvldisyn kmtdngivdi lntlkkspll rqlrifgnci
      361 ghpaakiikr mlmfqvleqe nidvrpyrvd hkwyfaryeg srckkeyhdi pyglhfnasr
      421 pptkinrpnm kyykytytkt telkvqhsii tiisrilsrq hvqdckccyc ikceaphfde
      481 lcrdanhped ctcckckads eisidksiid rlvpvidvmk sisyilkrvn cevqdkivrw
      541 vninedilee dletiacnre dvktssedtv lcntswvqln mptlqkylqq tssknvlivp
      601 knylvysnsa demsslqycp nksm
//
LOCUS       XP_034185135             546 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606586 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185135
VERSION     XP_034185135.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329244.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185135.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="uncharacterized protein LOC117606586 isoform X2"
                     /calculated_mol_wt=63306
     Region          <67..269
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          88..106
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          116..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          161..188
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          189..216
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          217..243
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          244..271
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          272..295
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..546
                     /gene="LOC117606586"
                     /coded_by="XM_034329244.2:197..1837"
                     /db_xref="GeneID:117606586"
ORIGIN      
        1 mkfnkyqyss cidpnrtnss lkehpdmkls cyyqlpnqnf fkcfctsics atkrldmkgs
       61 visekirrhf qdtdipalli flkkhpdiih inlanndisd tgfmnlldhv llyksvenld
      121 lqnnnitekg tnyllaraey leikylnlka nkfgvqnntc lralhlqkyn fschdienmm
      181 snakynntly lldlgnnnig dhgvehitsw likrpalktl ilcrniitdh garslsftlp
      241 fskilvldis ynkmtdngiv dilntlkksp llrqlrifgn cighpaakii krmlmfqvle
      301 qenidvrpyr vdhkwyfary egsrckkeyh dipyglhfna srpptkinrp nmkyykytyt
      361 kttelkvqhs iitiisrils rqhvqdckcc ycikceaphf delcrdanhp edctcckcka
      421 dseisidksi idrlvpvidv mksisyilkr vncevqdkiv rwvninedil eedletiacn
      481 redvktssed tvlcntswvq lnmptlqkyl qqtssknvli vpknylvysn sademsslqy
      541 cpnksm
//
LOCUS       XP_034185137             611 aa            linear   INV 03-SEP-2025
DEFINITION  PTEN-induced putative kinase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185137
VERSION     XP_034185137.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329246.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..611
                     /product="PTEN-induced putative kinase 1"
                     /calculated_mol_wt=68684
     Region          173..512
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(173..176,179,181,214,216,271,314..317,358,362..363,
                     365,381..382)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..611
                     /gene="Pink1"
                     /coded_by="XM_034329246.2:192..2027"
                     /db_xref="GeneID:117606587"
ORIGIN      
        1 msirtavyrf vqngrallhs irntqcfyhp riyhdkihvv qvgksqgylp qgsnnvsstg
       61 rhlgylgaha rrifvdnilk rvtnslaadl rrraasrlaf gdsapffalv gvslasgtgi
      121 ltkddelegv cweirdaisk lqwntpqndk nyetikdeek vialkdfvig paiakgcsav
      181 vyatrfkdes aldeekdeih iddktkdvta fplalkmmfn ydtesnalsi lrsmyretvp
      241 arkqlkneel aewelrmaer ksklpphpni vamyyvftdr vpalpgswgm ypdalparin
      301 pqgsgrnmsl fllmkrydit lsqyladhtv svresillla qllegvahln ahgiahrdlk
      361 tdnilldlse etdncpslvi tdfgccladk nhglylpynt hdidkggnaa lmapevitae
      421 pgpftsinyt kadlwtvgtl ayeifgmknp fhgdnaqris lknhnykekd lpplpshvpa
      481 vvsaliknll srslykrldt etaatvmqly lwapntwlri egklpssnev mqwllclttk
      541 vlcegrntmq peevlcnkni qgidyhsyqr slstrscgrr tmpeyqlias flgrvtlgni
      601 rnalkwiqqn a
//
LOCUS       XP_034185138             525 aa            linear   INV 03-SEP-2025
DEFINITION  cell division cycle 73 hyrax [Osmia lignaria lignaria].
ACCESSION   XP_034185138
VERSION     XP_034185138.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329247.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="cell division cycle 73 hyrax"
                     /calculated_mol_wt=59314
     Region          1..292
                     /region_name="CDC73_N"
                     /note="Paf1 complex subunit CDC73 N-terminal; pfam16050"
                     /db_xref="CDD:464994"
     Region          361..514
                     /region_name="CDC73_C"
                     /note="RNA pol II accessory factor, Cdc73 family,
                     C-terminal; pfam05179"
                     /db_xref="CDD:461570"
     CDS             1..525
                     /gene="hyx"
                     /coded_by="XM_034329247.2:6..1583"
                     /db_xref="GeneID:117606588"
ORIGIN      
        1 madplsllrq ynvnkkeiie renqiifgef swpknvktny ltygsgkegt pkeyytlecl
       61 lfllkhvqlt hpvyvrqaaa enipvvrrpd rkdllaylng etatsaaidk sapleiptqv
      121 krtaedgles gsskkprfee thvqkvkeql aarldapkea svtvdniksl seamsvekia
      181 aikakrlakk rttikenddi gmgsdlcvil mdvdgtkdiv srerqwrtra tilqssgkif
      241 aknifailqs ikareegrqk gpaaptpmvt prstpmrplp qpavynrydq erfirqkeet
      301 egfkidtmgt yhgmtlksvt egtnpavrkp ptnpsatpns gllptsaakl tpqqaaqnkr
      361 psrtpiiiip santslitmy nskdilqdlk yvsneekraq gskrenevll qrrkeggltv
      421 pyrvvdnpqk ltnadwervv avfvmgpawq fkgwpfdgnp veifskicaf hlkydemrld
      481 anvarwavtv ielsrtkrhl draalmvfwe hldkhmiknk phlrf
//
LOCUS       XP_076545593             517 aa            linear   INV 03-SEP-2025
DEFINITION  protein Red [Osmia lignaria lignaria].
ACCESSION   XP_076545593
VERSION     XP_076545593.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689478.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..517
                     /product="protein Red"
                     /calculated_mol_wt=58725
     Region          75..294
                     /region_name="RED_N"
                     /note="RED-like protein N-terminal region; pfam07808"
                     /db_xref="CDD:462273"
     Region          400..502
                     /region_name="RED_C"
                     /note="RED-like protein C-terminal region; pfam07807"
                     /db_xref="CDD:462272"
     CDS             1..517
                     /gene="LOC143305502"
                     /coded_by="XM_076689478.1:149..1702"
                     /db_xref="GeneID:143305502"
ORIGIN      
        1 mpetteedlg msvrltnddf rkllmtpras vpsatpasvp gtvrdasakt aqtpnisggs
       61 raelrrkkks fyaklkkqee dkmaelaeky rdrarerrdg tnqdyqaedp mnsasayrav
      121 apdlksgmda aerrrqmiqq skflggdmeh thlvkgldya llqkvrseie akeheqeqem
      181 eklvkpkdkv kekkegekkd demlfktkig rnvyrtimtm kskqiernel ftpgrmayvi
      241 elddentdvd ipttlirska dvpttdntpt lttndivink laqilsylrq gnrhgkkgkk
      301 nkdgrsrpdd mndmdiaprp qdesiygdig dyvptigkkd tnqsrekkkg syfdksenal
      361 dtddkantpq lpsitpsnte nnapkkgall nklaaepegy aecypgldem qdaiddsdde
      421 vdytkmdlgn kkgpigrwdf dtpeeyseym nnkealpkaa fqygvkmadg rrtrkynkek
      481 nekaeldrew qkiqnimnkr kpttvldggs efkvpry
//
LOCUS       XP_034185139             494 aa            linear   INV 03-SEP-2025
DEFINITION  polyadenylate-binding protein-interacting protein 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185139
VERSION     XP_034185139.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329248.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="polyadenylate-binding protein-interacting
                     protein 1 isoform X1"
                     /calculated_mol_wt=55560
     Region          <283..396
                     /region_name="MIF4G"
                     /note="MIF4G domain; cl02652"
                     /db_xref="CDD:413423"
     CDS             1..494
                     /gene="LOC117606589"
                     /coded_by="XM_034329248.2:165..1649"
                     /db_xref="GeneID:117606589"
ORIGIN      
        1 mnplggdgsg dgdaerrggg rgrgrgpwts aaqeipmlrr phhatsgiqt tgstesknte
       61 qskldksfed iiqnstlsvh aaefipksfs pkqspqqqqp ssmrfkhsvq drlqlareip
      121 lqaqnyqqve qvhqydnlsd rseyftnvqd ygrfdggsgd ykekhhdssg dednylmefa
      181 natqslmsvi hslilnpgrf dvivpplini lrpylespsq leeiikiiiq qsinegnfry
      241 ngarlccafl dtnltpgqqt mfketlyilc ksetdsqssn wqqkeehted eqkrchglil
      301 flaelvvqme htsafglgdl linliitvlk rpapnsvkni cqalklagqt lekdkgetrk
      361 emenmmralt elvtsgrvds higrmvhsvh elrngnwgqt ptlnssivep tetidpnqvl
      421 depvlygpdg kvltaeenkf ledvshstii edqvisehgy evekwlsede ddvdaayeef
      481 lkhipkqikn qtqk
//
LOCUS       XP_034185140             494 aa            linear   INV 03-SEP-2025
DEFINITION  polyadenylate-binding protein-interacting protein 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185140
VERSION     XP_034185140.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329249.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="polyadenylate-binding protein-interacting
                     protein 1 isoform X1"
                     /calculated_mol_wt=55560
     Region          <283..396
                     /region_name="MIF4G"
                     /note="MIF4G domain; cl02652"
                     /db_xref="CDD:413423"
     CDS             1..494
                     /gene="LOC117606589"
                     /coded_by="XM_034329249.2:150..1634"
                     /db_xref="GeneID:117606589"
ORIGIN      
        1 mnplggdgsg dgdaerrggg rgrgrgpwts aaqeipmlrr phhatsgiqt tgstesknte
       61 qskldksfed iiqnstlsvh aaefipksfs pkqspqqqqp ssmrfkhsvq drlqlareip
      121 lqaqnyqqve qvhqydnlsd rseyftnvqd ygrfdggsgd ykekhhdssg dednylmefa
      181 natqslmsvi hslilnpgrf dvivpplini lrpylespsq leeiikiiiq qsinegnfry
      241 ngarlccafl dtnltpgqqt mfketlyilc ksetdsqssn wqqkeehted eqkrchglil
      301 flaelvvqme htsafglgdl linliitvlk rpapnsvkni cqalklagqt lekdkgetrk
      361 emenmmralt elvtsgrvds higrmvhsvh elrngnwgqt ptlnssivep tetidpnqvl
      421 depvlygpdg kvltaeenkf ledvshstii edqvisehgy evekwlsede ddvdaayeef
      481 lkhipkqikn qtqk
//
LOCUS       XP_034185141             494 aa            linear   INV 03-SEP-2025
DEFINITION  polyadenylate-binding protein-interacting protein 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185141
VERSION     XP_034185141.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329250.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="polyadenylate-binding protein-interacting
                     protein 1 isoform X1"
                     /calculated_mol_wt=55560
     Region          <283..396
                     /region_name="MIF4G"
                     /note="MIF4G domain; cl02652"
                     /db_xref="CDD:413423"
     CDS             1..494
                     /gene="LOC117606589"
                     /coded_by="XM_034329250.2:137..1621"
                     /db_xref="GeneID:117606589"
ORIGIN      
        1 mnplggdgsg dgdaerrggg rgrgrgpwts aaqeipmlrr phhatsgiqt tgstesknte
       61 qskldksfed iiqnstlsvh aaefipksfs pkqspqqqqp ssmrfkhsvq drlqlareip
      121 lqaqnyqqve qvhqydnlsd rseyftnvqd ygrfdggsgd ykekhhdssg dednylmefa
      181 natqslmsvi hslilnpgrf dvivpplini lrpylespsq leeiikiiiq qsinegnfry
      241 ngarlccafl dtnltpgqqt mfketlyilc ksetdsqssn wqqkeehted eqkrchglil
      301 flaelvvqme htsafglgdl linliitvlk rpapnsvkni cqalklagqt lekdkgetrk
      361 emenmmralt elvtsgrvds higrmvhsvh elrngnwgqt ptlnssivep tetidpnqvl
      421 depvlygpdg kvltaeenkf ledvshstii edqvisehgy evekwlsede ddvdaayeef
      481 lkhipkqikn qtqk
//
LOCUS       XP_034185142             494 aa            linear   INV 03-SEP-2025
DEFINITION  polyadenylate-binding protein-interacting protein 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185142
VERSION     XP_034185142.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329251.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="polyadenylate-binding protein-interacting
                     protein 1 isoform X1"
                     /calculated_mol_wt=55560
     Region          <283..396
                     /region_name="MIF4G"
                     /note="MIF4G domain; cl02652"
                     /db_xref="CDD:413423"
     CDS             1..494
                     /gene="LOC117606589"
                     /coded_by="XM_034329251.2:26..1510"
                     /db_xref="GeneID:117606589"
ORIGIN      
        1 mnplggdgsg dgdaerrggg rgrgrgpwts aaqeipmlrr phhatsgiqt tgstesknte
       61 qskldksfed iiqnstlsvh aaefipksfs pkqspqqqqp ssmrfkhsvq drlqlareip
      121 lqaqnyqqve qvhqydnlsd rseyftnvqd ygrfdggsgd ykekhhdssg dednylmefa
      181 natqslmsvi hslilnpgrf dvivpplini lrpylespsq leeiikiiiq qsinegnfry
      241 ngarlccafl dtnltpgqqt mfketlyilc ksetdsqssn wqqkeehted eqkrchglil
      301 flaelvvqme htsafglgdl linliitvlk rpapnsvkni cqalklagqt lekdkgetrk
      361 emenmmralt elvtsgrvds higrmvhsvh elrngnwgqt ptlnssivep tetidpnqvl
      421 depvlygpdg kvltaeenkf ledvshstii edqvisehgy evekwlsede ddvdaayeef
      481 lkhipkqikn qtqk
//
LOCUS       XP_034185143             466 aa            linear   INV 03-SEP-2025
DEFINITION  polyadenylate-binding protein-interacting protein 1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034185143
VERSION     XP_034185143.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329252.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="polyadenylate-binding protein-interacting
                     protein 1 isoform X2"
                     /calculated_mol_wt=53153
     Region          <255..368
                     /region_name="MIF4G"
                     /note="MIF4G domain; cl02652"
                     /db_xref="CDD:413423"
     CDS             1..466
                     /gene="LOC117606589"
                     /coded_by="XM_034329252.2:466..1866"
                     /db_xref="GeneID:117606589"
ORIGIN      
        1 mlhleskqqv vrfisenkyi yysgsteskn teqskldksf ediiqnstls vhaaefipks
       61 fspkqspqqq qpssmrfkhs vqdrlqlare iplqaqnyqq veqvhqydnl sdrseyftnv
      121 qdygrfdggs gdykekhhds sgdednylme fanatqslms vihslilnpg rfdvivppli
      181 nilrpylesp sqleeiikii iqqsinegnf ryngarlcca fldtnltpgq qtmfketlyi
      241 lcksetdsqs snwqqkeeht edeqkrchgl ilflaelvvq mehtsafglg dllinliitv
      301 lkrpapnsvk nicqalklag qtlekdkget rkemenmmra ltelvtsgrv dshigrmvhs
      361 vhelrngnwg qtptlnssiv eptetidpnq vldepvlygp dgkvltaeen kfledvshst
      421 iiedqviseh gyevekwlse deddvdaaye eflkhipkqi knqtqk
//
LOCUS       XP_034185144             456 aa            linear   INV 03-SEP-2025
DEFINITION  polyadenylate-binding protein-interacting protein 1 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034185144
VERSION     XP_034185144.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329253.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..456
                     /product="polyadenylate-binding protein-interacting
                     protein 1 isoform X3"
                     /calculated_mol_wt=51056
     Region          <245..358
                     /region_name="MIF4G"
                     /note="MIF4G domain; cl02652"
                     /db_xref="CDD:413423"
     CDS             1..456
                     /gene="LOC117606589"
                     /coded_by="XM_034329253.2:164..1534"
                     /db_xref="GeneID:117606589"
ORIGIN      
        1 mnplggdgsg dgdaerrggg rgrgrgpwts aaqeipmlrr phhatsgiqt tgstesknte
       61 qskldksfed iiqnstlsvh aaefipksfs pkqspqqqqp ssmrfkhsvq drlqlareip
      121 lqaqnyqqds sgdednylme fanatqslms vihslilnpg rfdvivppli nilrpylesp
      181 sqleeiikii iqqsinegnf ryngarlcca fldtnltpgq qtmfketlyi lcksetdsqs
      241 snwqqkeeht edeqkrchgl ilflaelvvq mehtsafglg dllinliitv lkrpapnsvk
      301 nicqalklag qtlekdkget rkemenmmra ltelvtsgrv dshigrmvhs vhelrngnwg
      361 qtptlnssiv eptetidpnq vldepvlygp dgkvltaeen kfledvshst iiedqviseh
      421 gyevekwlse deddvdaaye eflkhipkqi knqtqk
//
LOCUS       XP_034185145             467 aa            linear   INV 03-SEP-2025
DEFINITION  TAR DNA-binding protein-43 homolog isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185145
VERSION     XP_034185145.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329254.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="TAR DNA-binding protein-43 homolog isoform X1"
                     /calculated_mol_wt=50468
     Region          4..77
                     /region_name="TDP43_N"
                     /note="Transactive response DNA-binding protein N-terminal
                     domain; pfam18694"
                     /db_xref="CDD:465833"
     Site            order(4,14..21,27,29..30,33..34,36..37)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:410991"
     Region          107..180
                     /region_name="RRM1_TDP43"
                     /note="RNA recognition motif 1 (RRM1) found in TAR
                     DNA-binding protein 43 (TDP-43) and similar proteins;
                     cd12321"
                     /db_xref="CDD:409760"
     Region          192..261
                     /region_name="RRM2_TDP43"
                     /note="RNA recognition motif 2 (RRM2) found in TAR
                     DNA-binding protein 43 (TDP-43) and similar proteins;
                     cd12322"
                     /db_xref="CDD:409761"
     Site            order(193,195,197..198,222,228,230,232,259..261)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:409761"
     CDS             1..467
                     /gene="TBPH"
                     /coded_by="XM_034329254.2:367..1770"
                     /db_xref="GeneID:117606590"
ORIGIN      
        1 mssyiqvaed egdepielpt eddstllltt vtaqfpgtcg lkyrnpesrt mrgirlvdgr
       61 lhppengwgk aiyfcvfpke nkrksddnle nstaktkrme tklrctdliv lglpwktteq
      121 nlreyfetfg evlmaqvkkd aksgqskgfg firfgsyesq lrclaqrhmi dgrwcdvkvp
      181 nskegmiqqv pckvfvgrct edltaddlrd yfskygevtd vfipkpfraf sfvtfldpev
      241 aqslcgedhi ikgvsvhvsn aapksegnnk nfnnqvnsnm rrgapgpven nvqgnyqgnr
      301 ymghsgnnmg pngwnnpgnr anldmpnlqa lgitgqnngg gggggmsnpl amgglnlsal
      361 pvnpalvaaa lnqaswglig nlqnqgndqg ysnqpgppgq ppsgnnsign sgnsgflswm
      421 nqgggdgntg npgtggpgpn npnasqgawq nhpgqrdqks ntflkye
//
LOCUS       XP_034185147             465 aa            linear   INV 03-SEP-2025
DEFINITION  TAR DNA-binding protein-43 homolog isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185147
VERSION     XP_034185147.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329256.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..465
                     /product="TAR DNA-binding protein-43 homolog isoform X2"
                     /calculated_mol_wt=54254
     Region          4..77
                     /region_name="TDP43_N"
                     /note="Transactive response DNA-binding protein N-terminal
                     domain; pfam18694"
                     /db_xref="CDD:465833"
     Site            order(4,14..21,27,29..30,33..34,36..37)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:410991"
     Region          107..180
                     /region_name="RRM1_TDP43"
                     /note="RNA recognition motif 1 (RRM1) found in TAR
                     DNA-binding protein 43 (TDP-43) and similar proteins;
                     cd12321"
                     /db_xref="CDD:409760"
     CDS             1..465
                     /gene="TBPH"
                     /coded_by="XM_034329256.2:367..1764"
                     /db_xref="GeneID:117606590"
ORIGIN      
        1 mssyiqvaed egdepielpt eddstllltt vtaqfpgtcg lkyrnpesrt mrgirlvdgr
       61 lhppengwgk aiyfcvfpke nkrksddnle nstaktkrme tklrctdliv lglpwktteq
      121 nlreyfetfg evlmaqvkkd aksgqskgfg firfgsyesq lrclaqrhmi dgrwcdvkvp
      181 nsknidglmm arqydtskhn ecyrpipvht pssrrvpvak pyinlntpea antprydsey
      241 dyktthypsy ppnypyddnt kykyegqnyn ayekanypsy edpgydtrny mvqhttaaap
      301 pnpaypsdte ytrytnyegr aavypvmenp dygeksryny gydrnyyead aryasstdcr
      361 ysldvrypdt rhgdnripad greadcrypv enremdrrya idgrdpegry avenrevdvr
      421 yptdtkevda rfadtpryps kenyydryyk hrpsrdhhvs dtsry
//
LOCUS       XP_034185148             453 aa            linear   INV 03-SEP-2025
DEFINITION  TAR DNA-binding protein-43 homolog isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185148
VERSION     XP_034185148.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329257.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="TAR DNA-binding protein-43 homolog isoform X3"
                     /calculated_mol_wt=48837
     Region          4..77
                     /region_name="TDP43_N"
                     /note="Transactive response DNA-binding protein N-terminal
                     domain; pfam18694"
                     /db_xref="CDD:465833"
     Site            order(4,14..21,27,29..30,33..34,36..37)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:410991"
     Region          93..166
                     /region_name="RRM1_TDP43"
                     /note="RNA recognition motif 1 (RRM1) found in TAR
                     DNA-binding protein 43 (TDP-43) and similar proteins;
                     cd12321"
                     /db_xref="CDD:409760"
     Region          178..247
                     /region_name="RRM2_TDP43"
                     /note="RNA recognition motif 2 (RRM2) found in TAR
                     DNA-binding protein 43 (TDP-43) and similar proteins;
                     cd12322"
                     /db_xref="CDD:409761"
     Site            order(179,181,183..184,208,214,216,218,245..247)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:409761"
     CDS             1..453
                     /gene="TBPH"
                     /coded_by="XM_034329257.2:367..1728"
                     /db_xref="GeneID:117606590"
ORIGIN      
        1 mssyiqvaed egdepielpt eddstllltt vtaqfpgtcg lkyrnpesrt mrgirlvdgr
       61 lhppengwgk aiyfcvfpka ktkrmetklr ctdlivlglp wktteqnlre yfetfgevlm
      121 aqvkkdaksg qskgfgfirf gsyesqlrcl aqrhmidgrw cdvkvpnske gmiqqvpckv
      181 fvgrctedlt addlrdyfsk ygevtdvfip kpfrafsfvt fldpevaqsl cgedhiikgv
      241 svhvsnaapk segnnknfnn qvnsnmrrga pgpvennvqg nyqgnrymgh sgnnmgpngw
      301 nnpgnranld mpnlqalgit gqnngggggg gmsnplamgg lnlsalpvnp alvaaalnqa
      361 swglignlqn qgndqgysnq pgppgqppsg nnsignsgns gflswmnqgg gdgntgnpgt
      421 ggpgpnnpna sqgawqnhpg qrdqksntfl kye
//
LOCUS       XP_034185149             441 aa            linear   INV 03-SEP-2025
DEFINITION  TAR DNA-binding protein-43 homolog isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185149
VERSION     XP_034185149.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329258.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="TAR DNA-binding protein-43 homolog isoform X4"
                     /calculated_mol_wt=48003
     Region          4..77
                     /region_name="TDP43_N"
                     /note="Transactive response DNA-binding protein N-terminal
                     domain; pfam18694"
                     /db_xref="CDD:465833"
     Site            order(4,14..21,27,29..30,33..34,36..37)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:410991"
     Region          107..180
                     /region_name="RRM1_TDP43"
                     /note="RNA recognition motif 1 (RRM1) found in TAR
                     DNA-binding protein 43 (TDP-43) and similar proteins;
                     cd12321"
                     /db_xref="CDD:409760"
     Region          192..261
                     /region_name="RRM2_TDP43"
                     /note="RNA recognition motif 2 (RRM2) found in TAR
                     DNA-binding protein 43 (TDP-43) and similar proteins;
                     cd12322"
                     /db_xref="CDD:409761"
     Site            order(193,195,197..198,222,228,230,232,259..261)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:409761"
     CDS             1..441
                     /gene="TBPH"
                     /coded_by="XM_034329258.2:367..1692"
                     /db_xref="GeneID:117606590"
ORIGIN      
        1 mssyiqvaed egdepielpt eddstllltt vtaqfpgtcg lkyrnpesrt mrgirlvdgr
       61 lhppengwgk aiyfcvfpke nkrksddnle nstaktkrme tklrctdliv lglpwktteq
      121 nlreyfetfg evlmaqvkkd aksgqskgfg firfgsyesq lrclaqrhmi dgrwcdvkvp
      181 nskegmiqqv pckvfvgrct edltaddlrd yfskygevtd vfipkpfraf sfvtfldpev
      241 aqslcgedhi ikgvsvhvsn aapksegnnk nfnnqvnsnm rrgapgpven nvqgnyqgnr
      301 ymghsgnnmg pngwnnpgnr anldmpnlqa lgitgqnngg gggggmsnpl amgglnlsal
      361 pvnpalvaaa lnqaswglig nlqnqgndqg ysnqpgppgq ppsgnnsigg pgpnnpnasq
      421 gawqnhpgqr dqksntflky e
//
LOCUS       XP_034185151             433 aa            linear   INV 03-SEP-2025
DEFINITION  histone deacetylase 3 [Osmia lignaria lignaria].
ACCESSION   XP_034185151
VERSION     XP_034185151.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329260.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="histone deacetylase 3"
                     /calculated_mol_wt=49258
     Region          5..385
                     /region_name="HDAC3"
                     /note="Histone deacetylase 3 (HDAC3); cd10005"
                     /db_xref="CDD:212529"
     Site            order(136..137,145..146,172,174,261,268,298,300)
                     /site_type="active"
                     /db_xref="CDD:212529"
     CDS             1..433
                     /gene="HDAC3"
                     /coded_by="XM_034329260.2:1..1302"
                     /db_xref="GeneID:117606592"
ORIGIN      
        1 msynkkvsyf ynpdvgnfhy gpghpmkphr lsvihslvln yglhkkmqiy rpyrasthdm
       61 crfhsdeyve flqrvtpqnl qgytkylshf nvgddcpvfe glfdfcsmyt gaslegatkl
      121 nnnccdiain wsgglhhakk feasgfcyin diviailell kyharvlyid idvhhgdgvq
      181 eafyltdrvm tvsfhkygny ffpgtgdmye igaesgryys vnvplkegid dasyvqvfkp
      241 vishvmeffq ptaivlqcga dslandrlgc fslstkghge cvkfvrdlnv plltvggggy
      301 tlrnvarcwt yetsllvdeq isnelpytey leyfapdftl hpdvvtrqdn anskqyleai
      361 trhvydnlkm iqhspsvqmq dvpcdalppe eerqpepdpd srqnvqdtdk ivepaneyys
      421 gekdqdkmdv ses
//
LOCUS       XP_034185152             414 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606593 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185152
VERSION     XP_034185152.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329261.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="uncharacterized protein LOC117606593 isoform X1"
                     /calculated_mol_wt=44395
     CDS             1..414
                     /gene="LOC117606593"
                     /coded_by="XM_034329261.2:337..1581"
                     /db_xref="GeneID:117606593"
ORIGIN      
        1 mmhlqtavlf llplvaivsa gyyqsriltp antvgceayt cgvnarcths egravcscmn
       61 lhmgdplsrc vrveclined cigskvctnn rcinpcdglc gvnalcevkn hlptcycppg
      121 htgdpftsch iadpqaackp spcgintkce vvneipvcsc spgyrgsplt gcrhecesds
      181 ecpghlacla sfkcespckc gtnaecqvin hqakctcphn wlgnpyvscr pectthsdcp
      241 rskpaclyqk clnpcegvcg vnadcnlrdi tpvcscprhm tgnpfvscrl feardlcepn
      301 pcgvnaictp ghdntgkerp vctcptgyig naltscqrge cltdsecpdn kacidftcrn
      361 pctgkecgpt avctprrhia vctcpdgtrg dalyncnpve srsvynygra cryc
//
LOCUS       XP_034185153             413 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606593 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185153
VERSION     XP_034185153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329262.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..413
                     /product="uncharacterized protein LOC117606593 isoform X2"
                     /calculated_mol_wt=44296
     CDS             1..413
                     /gene="LOC117606593"
                     /coded_by="XM_034329262.2:338..1579"
                     /db_xref="GeneID:117606593"
ORIGIN      
        1 mmhlqtavlf llplvaivsa gyyqsriltp antgceaytc gvnarcthse gravcscmnl
       61 hmgdplsrcv rveclinedc igskvctnnr cinpcdglcg vnalcevknh lptcycppgh
      121 tgdpftschi adpqaackps pcgintkcev vneipvcscs pgyrgspltg crhecesdse
      181 cpghlaclas fkcespckcg tnaecqvinh qakctcphnw lgnpyvscrp ectthsdcpr
      241 skpaclyqkc lnpcegvcgv nadcnlrdit pvcscprhmt gnpfvscrlf eardlcepnp
      301 cgvnaictpg hdntgkerpv ctcptgyign altscqrgec ltdsecpdnk acidftcrnp
      361 ctgkecgpta vctprrhiav ctcpdgtrgd alyncnpves rsvynygrac ryc
//
LOCUS       XP_034185156             389 aa            linear   INV 03-SEP-2025
DEFINITION  stomatin like 2 [Osmia lignaria lignaria].
ACCESSION   XP_034185156
VERSION     XP_034185156.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329265.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="stomatin like 2"
                     /calculated_mol_wt=43745
     Region          60..322
                     /region_name="HflC"
                     /note="Regulator of protease activity HflC,
                     stomatin/prohibitin superfamily [Posttranslational
                     modification, protein turnover, chaperones]; COG0330"
                     /db_xref="CDD:440099"
     CDS             1..389
                     /gene="Stoml2"
                     /coded_by="XM_034329265.2:124..1293"
                     /db_xref="GeneID:117606594"
ORIGIN      
        1 mllrtyswem ncrfkslarn fgilkhqpvf gfehpalklp itqlvrykse tpmntiilfv
       61 pqqeawiver mgkfhkvlep gfnvlmplid eiryvqslke laieipqqsa vtsdnvtlni
      121 dgvlylrvnd pylasygved pefavvqlaq ttmrselgki aldkvfrere glnvsivesi
      181 nkaseawgit clryeirdir lpprvqeamq mqveaerkkr aavlesegtr eaeiniaegk
      241 rlaqilasea akqeeinkan gkasaiiaia earakslklv gnalsltdak naaaysiaeq
      301 yvkafnelak vnntlilpsn vsdvssqvaq amtiykqvls rpsldngrkg trdasydvhd
      361 adesfeyfsd keeaekhres krkrnpeml
//
LOCUS       XP_034185157             385 aa            linear   INV 03-SEP-2025
DEFINITION  cathepsin D [Osmia lignaria lignaria].
ACCESSION   XP_034185157
VERSION     XP_034185157.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329266.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..385
                     /product="cathepsin D"
                     /calculated_mol_wt=42245
     Region          55..383
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(83,85,87,125..127,173)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133137"
     Site            83..85
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133137"
     Site            83
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133137"
     Site            order(125..128,132..135)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133137"
     CDS             1..385
                     /gene="cathD"
                     /coded_by="XM_034329266.2:183..1340"
                     /db_xref="GeneID:117606595"
ORIGIN      
        1 myrtiaffcv llvaanaelq rinlykmdsv rkqlkeygtd ltqvhvfqgn vppleplsny
       61 ldaqyygiin igtppqefrv ifdtgssnlw vpskkchltn iacklhhkyd stksytyken
      121 gtefsivygs gslsgylstd mvdvagtkis dqtfaealse pglafvaakf dgimgmaysr
      181 iavdgvmpvf ynmvkqglvp qpvfsfylnr dpdaksggem ilggsdpnhy vgpftyvpvd
      241 kkgywqfsmd rieigsnykv cengceaiad tgtsliggpv keielinkki gatplvagea
      301 lvdcnsipnl ptitfviggh sfplrgedyv fkvkqfgktv clsgfmgidi pppngplwil
      361 gdvfigryyt efdmgnnrvg faeak
//
LOCUS       XP_034185158             366 aa            linear   INV 03-SEP-2025
DEFINITION  protein sex-lethal isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185158
VERSION     XP_034185158.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329267.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185158.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="protein sex-lethal isoform X1"
                     /calculated_mol_wt=40081
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..366
                     /gene="LOC117606596"
                     /coded_by="XM_034329267.2:358..1458"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srgtggslsg ggrgrgvsgp
      301 pgmgmgisgs gpgmlgragg fgprggphgg figgsggpga mrmekihphr fnpigmgggy
      361 vqshfw
//
LOCUS       XP_034185159             366 aa            linear   INV 03-SEP-2025
DEFINITION  protein sex-lethal isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185159
VERSION     XP_034185159.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329268.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185159.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="protein sex-lethal isoform X1"
                     /calculated_mol_wt=40081
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..366
                     /gene="LOC117606596"
                     /coded_by="XM_034329268.2:410..1510"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srgtggslsg ggrgrgvsgp
      301 pgmgmgisgs gpgmlgragg fgprggphgg figgsggpga mrmekihphr fnpigmgggy
      361 vqshfw
//
LOCUS       XP_034185160             366 aa            linear   INV 03-SEP-2025
DEFINITION  protein sex-lethal isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185160
VERSION     XP_034185160.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329269.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185160.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="protein sex-lethal isoform X1"
                     /calculated_mol_wt=40081
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..366
                     /gene="LOC117606596"
                     /coded_by="XM_034329269.2:407..1507"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srgtggslsg ggrgrgvsgp
      301 pgmgmgisgs gpgmlgragg fgprggphgg figgsggpga mrmekihphr fnpigmgggy
      361 vqshfw
//
LOCUS       XP_076545594             366 aa            linear   INV 03-SEP-2025
DEFINITION  protein sex-lethal isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545594
VERSION     XP_076545594.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689479.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="protein sex-lethal isoform X1"
                     /calculated_mol_wt=40081
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..366
                     /gene="LOC117606596"
                     /coded_by="XM_076689479.1:295..1395"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srgtggslsg ggrgrgvsgp
      301 pgmgmgisgs gpgmlgragg fgprggphgg figgsggpga mrmekihphr fnpigmgggy
      361 vqshfw
//
LOCUS       XP_076545595             366 aa            linear   INV 03-SEP-2025
DEFINITION  protein sex-lethal isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545595
VERSION     XP_076545595.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689480.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="protein sex-lethal isoform X1"
                     /calculated_mol_wt=40081
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..366
                     /gene="LOC117606596"
                     /coded_by="XM_076689480.1:273..1373"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srgtggslsg ggrgrgvsgp
      301 pgmgmgisgs gpgmlgragg fgprggphgg figgsggpga mrmekihphr fnpigmgggy
      361 vqshfw
//
LOCUS       XP_034185161             359 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185161
VERSION     XP_034185161.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329270.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185161.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..359
                     /product="sex-lethal homolog isoform X2"
                     /calculated_mol_wt=39136
     Region          <89..264
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..359
                     /gene="LOC117606596"
                     /coded_by="XM_034329270.2:116..1195"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mfaasyweal sswstqqlpn sqptdadqqe qqnqtasgkv dqdpieltkm tdqreqqlqq
       61 qqhqqqpqql pqqqqqshqq mkgneeprtn liinylpqsm tekdlyslfv tigpvescrv
      121 mkdyktgysy gfgfvnyaka edaataintl nglqvqnkrl kvsfarpsge eiketnlyvt
      181 nlprnitesq iddifskygn ivqknilkdk itglprgvaf vrfdkreeaq eaiarlhgti
      241 peggseplsv kiaeehgkqk aayyagwqag ynqsrgtggs lsgggrgrgv sgppgmgmgi
      301 sgsgpgmlgr aggfgprggp hggfiggsgg pgamrmekih phrfnpigmg ggyvqshfw
//
LOCUS       XP_034185162             355 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185162
VERSION     XP_034185162.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329271.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185162.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="sex-lethal homolog isoform X3"
                     /calculated_mol_wt=38593
     Region          <85..260
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..355
                     /gene="LOC117606596"
                     /coded_by="XM_034329271.2:688..1755"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mvslgnisws tqqlpnsqpt dadqqeqqnq tasgkvdqdp ieltkmtdqr eqqlqqqqhq
       61 qqpqqlpqqq qqshqqmkgn eeprtnliin ylpqsmtekd lyslfvtigp vescrvmkdy
      121 ktgysygfgf vnyakaedaa taintlnglq vqnkrlkvsf arpsgeeike tnlyvtnlpr
      181 nitesqiddi fskygnivqk nilkdkitgl prgvafvrfd kreeaqeaia rlhgtipegg
      241 seplsvkiae ehgkqkaayy agwqagynqs rgtggslsgg grgrgvsgpp gmgmgisgsg
      301 pgmlgraggf gprggphggf iggsggpgam rmekihphrf npigmgggyv qshfw
//
LOCUS       XP_034185163             355 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185163
VERSION     XP_034185163.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329272.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185163.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="sex-lethal homolog isoform X3"
                     /calculated_mol_wt=38593
     Region          <85..260
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..355
                     /gene="LOC117606596"
                     /coded_by="XM_034329272.2:580..1647"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mvslgnisws tqqlpnsqpt dadqqeqqnq tasgkvdqdp ieltkmtdqr eqqlqqqqhq
       61 qqpqqlpqqq qqshqqmkgn eeprtnliin ylpqsmtekd lyslfvtigp vescrvmkdy
      121 ktgysygfgf vnyakaedaa taintlnglq vqnkrlkvsf arpsgeeike tnlyvtnlpr
      181 nitesqiddi fskygnivqk nilkdkitgl prgvafvrfd kreeaqeaia rlhgtipegg
      241 seplsvkiae ehgkqkaayy agwqagynqs rgtggslsgg grgrgvsgpp gmgmgisgsg
      301 pgmlgraggf gprggphggf iggsggpgam rmekihphrf npigmgggyv qshfw
//
LOCUS       XP_034185164             310 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185164
VERSION     XP_034185164.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329273.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="sex-lethal homolog isoform X4"
                     /calculated_mol_wt=33654
     Region          <40..215
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..310
                     /gene="LOC117606596"
                     /coded_by="XM_034329273.2:612..1544"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mtdqreqqlq qqqhqqqpqq lpqqqqqshq qmkgneeprt nliinylpqs mtekdlyslf
       61 vtigpvescr vmkdyktgys ygfgfvnyak aedaataint lnglqvqnkr lkvsfarpsg
      121 eeiketnlyv tnlprnites qiddifskyg nivqknilkd kitglprgva fvrfdkreea
      181 qeaiarlhgt ipeggsepls vkiaeehgkq kaayyagwqa gynqsrgtgg slsgggrgrg
      241 vsgppgmgmg isgsgpgmlg raggfgprgg phggfiggsg gpgamrmeki hphrfnpigm
      301 gggyvqshfw
//
LOCUS       XP_034185167             310 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185167
VERSION     XP_034185167.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329276.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="sex-lethal homolog isoform X4"
                     /calculated_mol_wt=33654
     Region          <40..215
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..310
                     /gene="LOC117606596"
                     /coded_by="XM_034329276.2:620..1552"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mtdqreqqlq qqqhqqqpqq lpqqqqqshq qmkgneeprt nliinylpqs mtekdlyslf
       61 vtigpvescr vmkdyktgys ygfgfvnyak aedaataint lnglqvqnkr lkvsfarpsg
      121 eeiketnlyv tnlprnites qiddifskyg nivqknilkd kitglprgva fvrfdkreea
      181 qeaiarlhgt ipeggsepls vkiaeehgkq kaayyagwqa gynqsrgtgg slsgggrgrg
      241 vsgppgmgmg isgsgpgmlg raggfgprgg phggfiggsg gpgamrmeki hphrfnpigm
      301 gggyvqshfw
//
LOCUS       XP_034185168             310 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185168
VERSION     XP_034185168.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329277.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="sex-lethal homolog isoform X4"
                     /calculated_mol_wt=33654
     Region          <40..215
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..310
                     /gene="LOC117606596"
                     /coded_by="XM_034329277.2:457..1389"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mtdqreqqlq qqqhqqqpqq lpqqqqqshq qmkgneeprt nliinylpqs mtekdlyslf
       61 vtigpvescr vmkdyktgys ygfgfvnyak aedaataint lnglqvqnkr lkvsfarpsg
      121 eeiketnlyv tnlprnites qiddifskyg nivqknilkd kitglprgva fvrfdkreea
      181 qeaiarlhgt ipeggsepls vkiaeehgkq kaayyagwqa gynqsrgtgg slsgggrgrg
      241 vsgppgmgmg isgsgpgmlg raggfgprgg phggfiggsg gpgamrmeki hphrfnpigm
      301 gggyvqshfw
//
LOCUS       XP_034185169             301 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034185169
VERSION     XP_034185169.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329278.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185169.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..301
                     /product="sex-lethal homolog isoform X5"
                     /calculated_mol_wt=34511
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..301
                     /gene="LOC117606596"
                     /coded_by="XM_034329278.2:353..1258"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srgerlyptr nkyqryahyl
      301 y
//
LOCUS       XP_034185170             294 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034185170
VERSION     XP_034185170.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329279.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185170.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="sex-lethal homolog isoform X6"
                     /calculated_mol_wt=33351
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..294
                     /gene="LOC117606596"
                     /coded_by="XM_034329279.2:358..1242"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srdmaasksk ysed
//
LOCUS       XP_034185171             293 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034185171
VERSION     XP_034185171.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329280.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185171.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="sex-lethal homolog isoform X7"
                     /calculated_mol_wt=33199
     Region          <96..290
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..293
                     /gene="LOC117606596"
                     /coded_by="XM_034329280.2:358..1239"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srdghknanv vll
//
LOCUS       XP_034185172             289 aa            linear   INV 03-SEP-2025
DEFINITION  sex-lethal homolog isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034185172
VERSION     XP_034185172.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329281.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185172.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="sex-lethal homolog isoform X8"
                     /calculated_mol_wt=32744
     Region          <96..271
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     CDS             1..289
                     /gene="LOC117606596"
                     /coded_by="XM_034329281.2:351..1220"
                     /db_xref="GeneID:117606596"
ORIGIN      
        1 mdflekkedt hnnmnpqtdw stqqlpnsqp tdadqqeqqn qtasgkvdqd pieltkmtdq
       61 reqqlqqqqh qqqpqqlpqq qqqshqqmkg neeprtnlii nylpqsmtek dlyslfvtig
      121 pvescrvmkd yktgysygfg fvnyakaeda ataintlngl qvqnkrlkvs farpsgeeik
      181 etnlyvtnlp rnitesqidd ifskygnivq knilkdkitg lprgvafvrf dkreeaqeai
      241 arlhgtipeg gseplsvkia eehgkqkaay yagwqagynq srghaklls
//
LOCUS       XP_034185195             359 aa            linear   INV 03-SEP-2025
DEFINITION  N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase-like [Osmia
            lignaria lignaria].
ACCESSION   XP_034185195
VERSION     XP_034185195.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329304.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185195.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..359
                     /product="N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase-
                     like"
                     /calculated_mol_wt=38943
     Region          30..331
                     /region_name="Glycosylasparaginase"
                     /note="Glycosylasparaginase and similar proteins; cd04513"
                     /db_xref="CDD:271335"
     Site            order(99..101,106,126..130,132..133,136..137,234..237,
                     242..244,248,263,266,272,276,294)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:271335"
     CDS             1..359
                     /gene="LOC117606611"
                     /coded_by="XM_034329304.2:8..1087"
                     /db_xref="GeneID:117606611"
ORIGIN      
        1 mkfllavcfi aqvclitsre vhasnssipl vvitwdykva tekawdvlyn qkrsaldaie
       61 esctycednr crktvgfggs pdesgettld alimdgvtmd vggvgllrni ksaasvarkv
      121 lehtkhsllg gelaskfave mgfkqeplqt eeskkmwldw kanncqpnfw knvvpdpskn
      181 cgpyravssm dfkmeehqsy vdeenhdtig vvaidskghi aagtstngar nkiagrigds
      241 piagagayad qqvgaaagtg dgdimmrflp sflaveemrr gaspkeaatt avkriaehyp
      301 tftgaviain kngnygaacn sltrfeyyva npelnkatml yancidaenq yqvvnarnn
//
LOCUS       XP_034185173             359 aa            linear   INV 03-SEP-2025
DEFINITION  doublesex- and mab-3-related transcription factor A2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185173
VERSION     XP_034185173.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329282.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..359
                     /product="doublesex- and mab-3-related transcription
                     factor A2"
                     /calculated_mol_wt=40120
     Region          22..68
                     /region_name="DM"
                     /note="DM DNA binding domain; pfam00751"
                     /db_xref="CDD:459924"
     Region          173..212
                     /region_name="CUE_DMA"
                     /note="CUE-like DMA domain found in the DM domain gene
                     family encodes putative transcription factors DMRTA1,
                     DMRTA2 and DMRTA3; cd14370"
                     /db_xref="CDD:270553"
     CDS             1..359
                     /gene="LOC117606597"
                     /coded_by="XM_034329282.2:1005..2084"
                     /db_xref="GeneID:117606597"
ORIGIN      
        1 mlrgknknkv aneeeesevk qrrpkcarcr nhgliswlrg hkrecryrec lcpkcsliae
       61 rqrvmaaqva lkrqqaaeda ialkmakvat gqklsrlppg kifgmsvtep ksiindnket
      121 dsgqkeeeeg aedskqdftq inqrqdspsi nengskyksl vfnnqeetke ssvsqtsvet
      181 larlfpntkl svlqlvlqrc gqdllkaiey fasdsfains tayasafrpp qtteetrste
      241 plsgtmlapf ysslsrniyg dgycllnivp dqfpksivsd ttactslpik tgqheeglaf
      301 nvqynnyfns gaqqqlrdhv yaqvtehlps rpgflhfppv ipgipcvqpn cpqcsykfp
//
LOCUS       XP_034185174             359 aa            linear   INV 03-SEP-2025
DEFINITION  doublesex- and mab-3-related transcription factor A2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185174
VERSION     XP_034185174.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329283.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..359
                     /product="doublesex- and mab-3-related transcription
                     factor A2"
                     /calculated_mol_wt=40120
     Region          22..68
                     /region_name="DM"
                     /note="DM DNA binding domain; pfam00751"
                     /db_xref="CDD:459924"
     Region          173..212
                     /region_name="CUE_DMA"
                     /note="CUE-like DMA domain found in the DM domain gene
                     family encodes putative transcription factors DMRTA1,
                     DMRTA2 and DMRTA3; cd14370"
                     /db_xref="CDD:270553"
     CDS             1..359
                     /gene="LOC117606597"
                     /coded_by="XM_034329283.2:774..1853"
                     /db_xref="GeneID:117606597"
ORIGIN      
        1 mlrgknknkv aneeeesevk qrrpkcarcr nhgliswlrg hkrecryrec lcpkcsliae
       61 rqrvmaaqva lkrqqaaeda ialkmakvat gqklsrlppg kifgmsvtep ksiindnket
      121 dsgqkeeeeg aedskqdftq inqrqdspsi nengskyksl vfnnqeetke ssvsqtsvet
      181 larlfpntkl svlqlvlqrc gqdllkaiey fasdsfains tayasafrpp qtteetrste
      241 plsgtmlapf ysslsrniyg dgycllnivp dqfpksivsd ttactslpik tgqheeglaf
      301 nvqynnyfns gaqqqlrdhv yaqvtehlps rpgflhfppv ipgipcvqpn cpqcsykfp
//
LOCUS       XP_034185175             316 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoporin Nup35 [Osmia lignaria lignaria].
ACCESSION   XP_034185175
VERSION     XP_034185175.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329284.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="nucleoporin Nup35"
                     /calculated_mol_wt=33929
     Region          159..231
                     /region_name="RRM_Nup53_like"
                     /note="RNA recognition motif (RRM) found in nucleoporin
                     Nup53 and similar proteins; cd12441"
                     /db_xref="CDD:409875"
     Site            order(161,163..164,194..196,215,217,222..225,227)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:409875"
     CDS             1..316
                     /gene="LOC117606598"
                     /coded_by="XM_034329284.2:144..1094"
                     /db_xref="GeneID:117606598"
ORIGIN      
        1 mepmtlgspv gspvqspgsp vasaylpsfl lgdsttpakv nlaspqetpk qlhighttls
       61 splshygtpd yrtsrqkavf gggntpntsq mvteshtggp ptrglfdtle tsqtsymspa
      121 nqsipcnqtr nlmntmnasi fndsslnpnk nesqgllqwv tvfgfppsdt ntvlahissr
      181 vrivdkhppp haqsnwihlk caseqeaqra lacngnivsg simvgvipct degvilgsdk
      241 enrsringsi kcfsnlsrln qspeystprt pvriqnarpl aagynqlspq svrvpenvpq
      301 kstglvskam eymfgw
//
LOCUS       XP_034185177             284 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquinone biosynthesis protein COQ3, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034185177
VERSION     XP_034185177.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329286.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="ubiquinone biosynthesis protein COQ3,
                     mitochondrial"
                     /calculated_mol_wt=31489
     Region          45..275
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Site            order(102..108,124..125,152..154,171)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..284
                     /gene="Coq3"
                     /coded_by="XM_034329286.2:275..1129"
                     /db_xref="GeneID:117606599"
ORIGIN      
        1 mkvvkllssr svstkstfrn rvkeqldqlp kppiikkstv dlatidhhsk lsnnwwdrng
       61 emralhalnp lrvqfvrdgl antgikaetp ylplegikil dvgcggglls eplarigaev
      121 tgidasseli svakehaald pnlsrklnyi qtsiedfapa nnktynaiia sevvehvnek
      181 elflkhcidt lkpggsiflt tlnktlpawl ggiiaaehvl klvpkgthdw nkfvtpaemq
      241 cllekcgckt klihgmfynp lknewfwias rainyaihav krke
//
LOCUS       XP_034185178             270 aa            linear   INV 03-SEP-2025
DEFINITION  lysoplasmalogenase TMEM86A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185178
VERSION     XP_034185178.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329287.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="lysoplasmalogenase TMEM86A isoform X1"
                     /calculated_mol_wt=30025
     Region          56..236
                     /region_name="YhhN"
                     /note="YhhN family; pfam07947"
                     /db_xref="CDD:429751"
     CDS             1..270
                     /gene="LOC117606600"
                     /coded_by="XM_034329287.2:239..1051"
                     /db_xref="GeneID:117606600"
ORIGIN      
        1 mlrccvaysl atflfvykll itnlksigpk lvpffksvsv yfillaeqps lltacfkclp
       61 iislivfvll hgislsqeyt fsrriltgli fscigdallv wpscftpgmc mfamaqimyi
      121 safgfiplni mlgtilyamc slviyalmpg lngvlvvgvp iyiillttma wraisrvqfy
      181 kelwtwtklc scigsicflv sdtllgfhyf htplpysqvs imltyyaaql gialsavgsk
      241 nnsiisnnnn nnnnnnnare dkneanlmrd
//
LOCUS       XP_034185179             270 aa            linear   INV 03-SEP-2025
DEFINITION  lysoplasmalogenase TMEM86A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185179
VERSION     XP_034185179.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329288.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="lysoplasmalogenase TMEM86A isoform X1"
                     /calculated_mol_wt=30025
     Region          56..236
                     /region_name="YhhN"
                     /note="YhhN family; pfam07947"
                     /db_xref="CDD:429751"
     CDS             1..270
                     /gene="LOC117606600"
                     /coded_by="XM_034329288.2:239..1051"
                     /db_xref="GeneID:117606600"
ORIGIN      
        1 mlrccvaysl atflfvykll itnlksigpk lvpffksvsv yfillaeqps lltacfkclp
       61 iislivfvll hgislsqeyt fsrriltgli fscigdallv wpscftpgmc mfamaqimyi
      121 safgfiplni mlgtilyamc slviyalmpg lngvlvvgvp iyiillttma wraisrvqfy
      181 kelwtwtklc scigsicflv sdtllgfhyf htplpysqvs imltyyaaql gialsavgsk
      241 nnsiisnnnn nnnnnnnare dkneanlmrd
//
LOCUS       XP_034185180             254 aa            linear   INV 03-SEP-2025
DEFINITION  lysoplasmalogenase TMEM86A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185180
VERSION     XP_034185180.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329289.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="lysoplasmalogenase TMEM86A isoform X2"
                     /calculated_mol_wt=28091
     Region          40..220
                     /region_name="YhhN"
                     /note="YhhN family; pfam07947"
                     /db_xref="CDD:429751"
     CDS             1..254
                     /gene="LOC117606600"
                     /coded_by="XM_034329289.2:554..1318"
                     /db_xref="GeneID:117606600"
ORIGIN      
        1 mssptqvlks igpklvpffk svsvyfilla eqpslltacf kclpiisliv fvllhgisls
       61 qeytfsrril tglifscigd allvwpscft pgmcmfamaq imyisafgfi plnimlgtil
      121 yamcslviya lmpglngvlv vgvpiyiill ttmawraisr vqfykelwtw tklcscigsi
      181 cflvsdtllg fhyfhtplpy sqvsimltyy aaqlgialsa vgsknnsiis nnnnnnnnnn
      241 naredknean lmrd
//
LOCUS       XP_034185181             268 aa            linear   INV 03-SEP-2025
DEFINITION  thioredoxin-related transmembrane protein 2 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034185181
VERSION     XP_034185181.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329290.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="thioredoxin-related transmembrane protein 2
                     homolog"
                     /calculated_mol_wt=30906
     Region          96..247
                     /region_name="TMX2"
                     /note="TMX2 family; composed of proteins similar to human
                     TMX2, a 372-amino acid TRX-related transmembrane protein,
                     identified and characterized through the cloning of its
                     cDNA from a human fetal library. It contains a TRX domain
                     but the redox active CXXC motif...; cd02962"
                     /db_xref="CDD:239260"
     CDS             1..268
                     /gene="LOC117606601"
                     /coded_by="XM_034329290.2:362..1168"
                     /db_xref="GeneID:117606601"
ORIGIN      
        1 mpfkkdlrll lkpyylinil lsvsyivskr lpivchyvfa qaeceldgre teilfflmiv
       61 imirtrktgs vtminylsss fvytkianli lwfyadirmg iifaiifilc gllfpeptyq
      121 gpenvtylrg aqglqeelyr dtrivwlvaf ytawnpacvn fapifselsa eyalenlkfg
      181 kvdigrypda gvkyhisdas tskqlptlil fkegkeverr pyadhkgklv kflfsldnik
      241 atfdlnnvyk dckknpikkk ekkmikae
//
LOCUS       XP_034185183             230 aa            linear   INV 03-SEP-2025
DEFINITION  GID complex subunit 8 homolog protein Houki [Osmia lignaria
            lignaria].
ACCESSION   XP_034185183
VERSION     XP_034185183.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329292.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="GID complex subunit 8 homolog protein Houki"
                     /calculated_mol_wt=26431
     Region          30..53
                     /region_name="LisH"
                     /note="pfam08513"
                     /db_xref="CDD:462501"
     Region          64..208
                     /region_name="CTLH"
                     /note="CTLH/CRA C-terminal to LisH motif domain;
                     pfam10607"
                     /db_xref="CDD:402305"
     CDS             1..230
                     /gene="Hou"
                     /coded_by="XM_034329292.2:158..850"
                     /db_xref="GeneID:117606602"
ORIGIN      
        1 msfpekqdni skdewvakle enshtlrvsm nnlimnylvt egfkeaaekf qqesgveptv
       61 dlsslddrir ireaiqngri qeatdlvnql hpelldndry lyfhlqqlhl ielirtgkie
      121 ealqfaqdrl seagesddni lcelertlal lafdephksp ysdllhpthr qkiaselnaa
      181 ilkmeykest sprvnnllkm ilwaqdelek kkvkypkmtd lgsatiespk
//
LOCUS       XP_076545598             228 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-ketoglutarate-dependent dioxygenase alkB homolog 7,
            mitochondrial isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545598
VERSION     XP_076545598.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689483.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="alpha-ketoglutarate-dependent dioxygenase alkB
                     homolog 7, mitochondrial isoform X1"
                     /calculated_mol_wt=26836
     CDS             1..228
                     /gene="LOC143305503"
                     /coded_by="XM_076689483.1:167..853"
                     /db_xref="GeneID:143305503"
ORIGIN      
        1 mlratlslft fsinknfnsi tlryymqtkq vcnkadrnve nwkeelcntm qifpnfitve
       61 eenslmeeie pyikklryeh shwddaihay reternkwne nnlkiinkir ekafpkgmsq
      121 iplihvldla pegwikphvd sirfcgeiia glsllsnsvm rltmveneal ykhdfllpqr
      181 slyimsgtar ynykheilkn eesyfegqpv sktrrisvic rsqpnpdt
//
LOCUS       XP_076545599             206 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-ketoglutarate-dependent dioxygenase alkB homolog 7,
            mitochondrial isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545599
VERSION     XP_076545599.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689484.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..206
                     /product="alpha-ketoglutarate-dependent dioxygenase alkB
                     homolog 7, mitochondrial isoform X2"
                     /calculated_mol_wt=24297
     CDS             1..206
                     /gene="LOC143305503"
                     /coded_by="XM_076689484.1:129..749"
                     /db_xref="GeneID:143305503"
ORIGIN      
        1 myymqtkqvc nkadrnvenw keelcntmqi fpnfitveee nslmeeiepy ikklryehsh
       61 wddaihayre ternkwnenn lkiinkirek afpkgmsqip lihvldlape gwikphvdsi
      121 rfcgeiiagl sllsnsvmrl tmvenealyk hdfllpqrsl yimsgtaryn ykheilknee
      181 syfegqpvsk trrisvicrs qpnpdt
//
LOCUS       XP_076545600             203 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-ketoglutarate-dependent dioxygenase alkB homolog 7,
            mitochondrial isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545600
VERSION     XP_076545600.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689485.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..203
                     /product="alpha-ketoglutarate-dependent dioxygenase alkB
                     homolog 7, mitochondrial isoform X3"
                     /calculated_mol_wt=23839
     CDS             1..203
                     /gene="LOC143305503"
                     /coded_by="XM_076689485.1:148..759"
                     /db_xref="GeneID:143305503"
ORIGIN      
        1 mqtkqvcnka drnvenwkee lcntmqifpn fitveeensl meeiepyikk lryehshwdd
       61 aihayreter nkwnennlki inkirekafp kgmsqiplih vldlapegwi kphvdsirfc
      121 geiiaglsll snsvmrltmv enealykhdf llpqrslyim sgtarynykh eilkneesyf
      181 egqpvsktrr isvicrsqpn pdt
//
LOCUS       XP_034185187             217 aa            linear   INV 03-SEP-2025
DEFINITION  sugar transporter SWEET1 [Osmia lignaria lignaria].
ACCESSION   XP_034185187
VERSION     XP_034185187.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329296.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="sugar transporter SWEET1"
                     /calculated_mol_wt=24062
     Region          10..93
                     /region_name="MtN3_slv"
                     /note="Sugar efflux transporter for intercellular
                     exchange; cl46784"
                     /db_xref="CDD:481124"
     Region          126..206
                     /region_name="MtN3_slv"
                     /note="Sugar efflux transporter for intercellular
                     exchange; cl46784"
                     /db_xref="CDD:481124"
     CDS             1..217
                     /gene="slv"
                     /coded_by="XM_034329296.2:60..713"
                     /db_xref="GeneID:117606603"
ORIGIN      
        1 mtstemkdal astasictvl qflagvlvcr kiikngttgn ssalafvtcy tscvlwmryg
       61 mlisdrfvll vnvfgtilqa syvcvfilys vkkfkivkqm iaatcflgvv yfysfyeedk
      121 vlaaryvgfl sctvtvlffa splmmvahvi rvknteslpf piimaslivs aqwfaygcli
      181 ndsflqipnf lgcvlsafql cfflvyrsge tieahli
//
LOCUS       XP_034185188             196 aa            linear   INV 03-SEP-2025
DEFINITION  GTP-binding protein Di-Ras2 [Osmia lignaria lignaria].
ACCESSION   XP_034185188
VERSION     XP_034185188.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329297.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="GTP-binding protein Di-Ras2"
                     /calculated_mol_wt=21600
     Region          7..172
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            14..21
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206711"
     Site            order(16..22,64,122..123,125,153..155)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206711"
     Site            39
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206711"
     Site            42..44
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206711"
     Site            61..64
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206711"
     Site            order(63..64,80..81)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206711"
     Site            122..125
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206711"
     Site            153..155
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206711"
     CDS             1..196
                     /gene="LOC117606604"
                     /coded_by="XM_034329297.2:1..591"
                     /db_xref="GeneID:117606604"
ORIGIN      
        1 mpeqsndyrv vvfgaggvgk sslvlrfvkg tfresyipti edtyrqvisc nknictlqit
       61 dttgshqfpa mqrlsiskgh afilvysvcs rqsleelrpi waiirelkgq disqipimlv
      121 gnkcdespsv revsisegaa eaanwgcgfl etsaktnhnv nalfrdllml eknrsvslqp
      181 vqsnnaislk ekcsvm
//
LOCUS       XP_034185189             191 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606605 [Osmia lignaria lignaria].
ACCESSION   XP_034185189
VERSION     XP_034185189.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329298.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185189.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..191
                     /product="uncharacterized protein LOC117606605"
                     /calculated_mol_wt=22064
     CDS             1..191
                     /gene="LOC117606605"
                     /coded_by="XM_034329298.2:435..1010"
                     /db_xref="GeneID:117606605"
ORIGIN      
        1 mrpdsrsvsf tfhrevlsvk nsseltknpk nlrrrlqdrf dkekpgdsck ekmskdnrsw
       61 nnltlgnmsa dsrralrsga eklsktissv rttfgtisqk fksstrrrqr leeqqspnnm
      121 skmqtpqtrs rqilgrtptk lyspfgiesp rhawdkenne tpmhtpvssn ahyiqcrpfr
      181 ftrakgfsvl r
//
LOCUS       XP_034185190             131 aa            linear   INV 03-SEP-2025
DEFINITION  protein KRTCAP2 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034185190
VERSION     XP_034185190.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329299.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..131
                     /product="protein KRTCAP2 homolog"
                     /calculated_mol_wt=14414
     Region          3..131
                     /region_name="Keratin_assoc"
                     /note="Keratinocyte-associated protein 2; pfam09775"
                     /db_xref="CDD:401649"
     CDS             1..131
                     /gene="LOC117606606"
                     /coded_by="XM_034329299.2:103..498"
                     /db_xref="GeneID:117606606"
ORIGIN      
        1 mpvssgasfi lssiltvlvf sgmqiyrnwl assqlytilg gyigsilfmc vltaignles
       61 tifgksfqqk lfpevmfsli vsliasglvh rvatttcflf smialyyinr isqetyavpv
      121 ptvqmhtkkr k
//
LOCUS       XP_034185192             117 aa            linear   INV 03-SEP-2025
DEFINITION  chemosensory protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185192
VERSION     XP_034185192.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329301.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..117
                     /product="chemosensory protein 1"
                     /calculated_mol_wt=13658
     Region          23..115
                     /region_name="OS-D"
                     /note="Insect pheromone-binding family, A10/OS-D;
                     pfam03392"
                     /db_xref="CDD:460908"
     CDS             1..117
                     /gene="CSP1"
                     /coded_by="XM_034329301.2:352..705"
                     /db_xref="GeneID:117606607"
ORIGIN      
        1 mrgyfvllal sllvwatqae dtysdkfdyi dveeiltndr lreqyykcfm dtapcmtada
       61 tffkgnifda mvtkcskcte kqkmfldkvt dwftknqpek wqafieksle dakkkai
//
LOCUS       XP_034185193             116 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin polymerization-promoting protein homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034185193
VERSION     XP_034185193.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329302.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="tubulin polymerization-promoting protein
                     homolog"
                     /calculated_mol_wt=13339
     CDS             1..116
                     /gene="LOC117606609"
                     /coded_by="XM_034329302.2:77..427"
                     /db_xref="GeneID:117606609"
ORIGIN      
        1 meefglkkkd epeasfeeif qtycemdpvs ssqnvdlipl sqsdrwlisa rildmitltt
       61 tdtglaffkf rkralsydey ltylndlann ynldletmkq kmqtcgrpkq tastim
//
LOCUS       XP_034185194              89 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light chain 2, cytoplasmic [Osmia lignaria lignaria].
ACCESSION   XP_034185194
VERSION     XP_034185194.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329303.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="dynein light chain 2, cytoplasmic"
                     /calculated_mol_wt=10213
     Region          5..88
                     /region_name="DLC-like_DYNLL1_DYNLL2"
                     /note="dynein light chain (DLC)-like domain found in the
                     cytoplasmic dynein light chain 1 (DYNLL1) family; cd21452"
                     /db_xref="CDD:412000"
     CDS             1..89
                     /gene="LOC117606610"
                     /coded_by="XM_034329303.2:183..452"
                     /db_xref="GeneID:117606610"
ORIGIN      
        1 msdrkavikn admsedmqqd avdcatqale kyniekdiaa fikkefdkky nptwhcivgr
       61 nfgsyvthet rhfiyfylgq vaillfksg
//
LOCUS       XP_034190259            1136 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent RNA helicase l(1)G0007 [Osmia lignaria lignaria].
ACCESSION   XP_034190259
VERSION     XP_034190259.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334368.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1136
                     /product="ATP-dependent RNA helicase l(1)G0007"
                     /calculated_mol_wt=129571
     Region          441..>1056
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     CDS             1..1136
                     /gene="l(1)G0007"
                     /coded_by="XM_034334368.2:160..3570"
                     /db_xref="GeneID:117608777"
ORIGIN      
        1 mdtsndsaly rlegtdknqt gglvirkkps dhtfkkpqas vlgldklakr krqenaqesk
       61 sfksesgspk seskerkyrs yaeetpthtg gvnyeaqkrl esrlkhqrls aqeknyrhgh
      121 kdrdrdrnrd rersrsrdsn rqtplrfkde pqtpvfktrd ptsksnwddd edeeprkssw
      181 dhptpnlyss rdgrdsirse ftpsykynsw ikdrkasgat pridgeekel weeeqqrldr
      241 ewyalddgen hafadvseey trkkemelea krqkrlsaqq rqinkdnelw ernrmltsgv
      301 vssldhdddp ddegetrvhl lvhnvvppfl dgrivftkqp epvvpvrdpt sdmalvarkg
      361 salvrayreq kerkraqkkh welagthign imgvrdrhkd dreepgqetd fkagqkyarh
      421 ikgdevtgea kyrsiqhqrr slpvfavrqe llnvirensv vvivgetgsg kttqltqylh
      481 edgysrygii gctqprrvaa msvakrvsde mattlgekvg yairfedcts kdtvikymtd
      541 gillreslre gdldrysvii mdeahersls tdvlfgllre vvarrhdlkl ivtsatmdss
      601 kfstffgnaa tfqipgrtfp vevlhaknsv edyvdaavkq vlqihlqprs gdvlvfmpgq
      661 edievtceal kerlaeiesa pplsilpiys qlpsdlqaki fqrsegglrk cvvatniaet
      721 sltvdgivfv vdsgycklkv ynprigmdal qvypvsrana dqragragrt gpgtcyrlyt
      781 rrqyldelll tgvpeiqrtn lantvlllks lgvqdllafh fmdpppqdni lnslyqlwil
      841 galdhtgrlt plgrqmaefp ldppqcqmli vasqlgctad iliivsmlsv psifyrpkgr
      901 eedsdsarek fqvpesdhlt ylnvynqwka ngyssswcnd hfihakamrk vrevrqqlee
      961 ilkqqkmevv scgtdwdvvr kcicsayfhq aarlkgigey vncrtgmpch lhptsalfgm
     1021 gftpdyvvyh elvmtakeym qcvtavdghw laelgpmffs vketgrsgra krrqamqhlh
     1081 emegqmkeae eemkaraqeq lereqasirk qeiltpgire pgtpapyrkt pgrlgl
//
LOCUS       XP_034190260             241 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L23A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190260
VERSION     XP_034190260.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334369.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190260.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="ribosomal protein L23A isoform X1"
                     /calculated_mol_wt=25875
     Region          104..241
                     /region_name="PTZ00191"
                     /note="60S ribosomal protein L23a; Provisional"
                     /db_xref="CDD:185507"
     CDS             1..241
                     /gene="RpL23A"
                     /coded_by="XM_034334369.2:80..805"
                     /db_xref="GeneID:117608778"
ORIGIN      
        1 mapkakptdk aagkssekka ekktekkpat aastskvtkp aakpataakk aapaksasat
       61 kpaakpaaka apkvaakpva akpkkptqpa kktpkggksa aplqkalkaq kkilkgvhgs
      121 rvrkirtsvh fhrpktfrpq rnpkyprkcv psrnrmdafn iikfplttea amkkiednnt
      181 lvfivhtlan kyhikssikk lydvdvakvn tligpdgkkk ayvrltrdyd aldvankigi
      241 i
//
LOCUS       XP_034190261             240 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L23A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190261
VERSION     XP_034190261.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334370.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190261.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="ribosomal protein L23A isoform X2"
                     /calculated_mol_wt=25804
     Region          103..240
                     /region_name="PTZ00191"
                     /note="60S ribosomal protein L23a; Provisional"
                     /db_xref="CDD:185507"
     CDS             1..240
                     /gene="RpL23A"
                     /coded_by="XM_034334370.2:44..766"
                     /db_xref="GeneID:117608778"
ORIGIN      
        1 mapkakptdk agkssekkae kktekkpata astskvtkpa akpataakka apaksasatk
       61 paakpaakaa pkvaakpvaa kpkkptqpak ktpkggksaa plqkalkaqk kilkgvhgsr
      121 vrkirtsvhf hrpktfrpqr npkyprkcvp srnrmdafni ikfpltteaa mkkiednntl
      181 vfivhtlank yhikssikkl ydvdvakvnt ligpdgkkka yvrltrdyda ldvankigii
//
LOCUS       XP_034190262             931 aa            linear   INV 03-SEP-2025
DEFINITION  coatomer subunit beta' [Osmia lignaria lignaria].
ACCESSION   XP_034190262
VERSION     XP_034190262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..931
                     /product="coatomer subunit beta'"
                     /calculated_mol_wt=104565
     Region          8..297
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(14,32,36,42..43,55..56,74,78,84..85,97..98,115,120,
                     126..127,141,159,164,170..171,183..184,204,208,214..215,
                     227..228,245,250,256..257,269..270,288,292)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          19..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          62..97
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          145..182
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          189..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          304..773
                     /region_name="Coatomer_WDAD_beta-like"
                     /note="Coatomer WD Associated Region from Coatomer Subunit
                     Beta and Beta'; cd22947"
                     /db_xref="CDD:438572"
     Region          304..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          353..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          393..425
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          431..462
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          466..514
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          518..557
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Site            order(551,581,700..702,729..730,751..752,755)
                     /site_type="other"
                     /note="coatomer beta'-subunit binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438572"
     Region          558..582
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     CDS             1..931
                     /gene="beta'COP"
                     /coded_by="XM_034334371.2:389..3184"
                     /db_xref="GeneID:117608779"
ORIGIN      
        1 mplrldikrk ltarsdrvks vdlhptepwm lcslyqgnvn iwnhetqmlv ktfevcdlpv
       61 rtakfvprkn wvvtgsddmq irvfnyntle rvhsfeahsd yvrciavhpt qpfiltssdd
      121 mliklwnwek awigqqvfeg hthyvmqivf npkdnntfas asldrtvkvw qlgsstanft
      181 leghekgvnc vdyyhggdkp ylisgaddky vkiwdyqnkt cvqtleghtq nisavcfhpe
      241 lpiiltgsed gtvriwhagt yrlesslnyg fervwtiscm rgsnnvaigy degsvmvkvg
      301 reepavsmds lggkivwakh seiqqvnlka lgeeaqdger lplavkdmga ceiypqtiqh
      361 npngrflvvc gdgeyiiyts malrnkafgq asefvwaads sqyavresnt tvkvfknfke
      421 kksfkpdfga dgifggfllg vssgsglsff dwdtlklvrr idiqpthvyw aenaslvtla
      481 ttdqyfilky hgdavvnape nsediedafe mvaemnevvk tglwvgdcfi ytnsvnrvny
      541 fvggeivtvs hldrpmyllg yvprdnrlyl cdkelsvvsy slllsvleyq tavmrkdfet
      601 adrvlptvpk ehrtrvahfl ekqgfkeqal avstdpehrf elalalgdla tahalakean
      661 sqqkwrqlas latqkgklcl aqeclhqaqd fggllllats tgnanmiqkl gtdanetgkn
      721 nisflsyfil gdvdkcldil iktdripeaa ffartyapsr issivklwke klsavsekag
      781 qsladpeqye nlfpgyreal kaeqflreds kkkipasafp nvqsnierkp feemlaaeea
      841 nqftykagpt tnneaevapt evltnrlqdl diskvipsst ekasvpekvt ktnsnsrplt
      901 vddddldldf eldetldttg vsldddllee d
//
LOCUS       XP_076545602             931 aa            linear   INV 03-SEP-2025
DEFINITION  coatomer subunit beta' [Osmia lignaria lignaria].
ACCESSION   XP_076545602
VERSION     XP_076545602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689487.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..931
                     /product="coatomer subunit beta'"
                     /calculated_mol_wt=104565
     Region          8..297
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(14,32,36,42..43,55..56,74,78,84..85,97..98,115,120,
                     126..127,141,159,164,170..171,183..184,204,208,214..215,
                     227..228,245,250,256..257,269..270,288,292)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          19..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          62..97
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          145..182
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          189..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          304..773
                     /region_name="Coatomer_WDAD_beta-like"
                     /note="Coatomer WD Associated Region from Coatomer Subunit
                     Beta and Beta'; cd22947"
                     /db_xref="CDD:438572"
     Region          304..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          353..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          393..425
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          431..462
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          466..514
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          518..557
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Site            order(551,581,700..702,729..730,751..752,755)
                     /site_type="other"
                     /note="coatomer beta'-subunit binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438572"
     Region          558..582
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     CDS             1..931
                     /gene="beta'COP"
                     /coded_by="XM_076689487.1:260..3055"
                     /db_xref="GeneID:117608779"
ORIGIN      
        1 mplrldikrk ltarsdrvks vdlhptepwm lcslyqgnvn iwnhetqmlv ktfevcdlpv
       61 rtakfvprkn wvvtgsddmq irvfnyntle rvhsfeahsd yvrciavhpt qpfiltssdd
      121 mliklwnwek awigqqvfeg hthyvmqivf npkdnntfas asldrtvkvw qlgsstanft
      181 leghekgvnc vdyyhggdkp ylisgaddky vkiwdyqnkt cvqtleghtq nisavcfhpe
      241 lpiiltgsed gtvriwhagt yrlesslnyg fervwtiscm rgsnnvaigy degsvmvkvg
      301 reepavsmds lggkivwakh seiqqvnlka lgeeaqdger lplavkdmga ceiypqtiqh
      361 npngrflvvc gdgeyiiyts malrnkafgq asefvwaads sqyavresnt tvkvfknfke
      421 kksfkpdfga dgifggfllg vssgsglsff dwdtlklvrr idiqpthvyw aenaslvtla
      481 ttdqyfilky hgdavvnape nsediedafe mvaemnevvk tglwvgdcfi ytnsvnrvny
      541 fvggeivtvs hldrpmyllg yvprdnrlyl cdkelsvvsy slllsvleyq tavmrkdfet
      601 adrvlptvpk ehrtrvahfl ekqgfkeqal avstdpehrf elalalgdla tahalakean
      661 sqqkwrqlas latqkgklcl aqeclhqaqd fggllllats tgnanmiqkl gtdanetgkn
      721 nisflsyfil gdvdkcldil iktdripeaa ffartyapsr issivklwke klsavsekag
      781 qsladpeqye nlfpgyreal kaeqflreds kkkipasafp nvqsnierkp feemlaaeea
      841 nqftykagpt tnneaevapt evltnrlqdl diskvipsst ekasvpekvt ktnsnsrplt
      901 vddddldldf eldetldttg vsldddllee d
//
LOCUS       XP_034190263             931 aa            linear   INV 03-SEP-2025
DEFINITION  coatomer subunit beta' [Osmia lignaria lignaria].
ACCESSION   XP_034190263
VERSION     XP_034190263.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334372.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..931
                     /product="coatomer subunit beta'"
                     /calculated_mol_wt=104565
     Region          8..297
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(14,32,36,42..43,55..56,74,78,84..85,97..98,115,120,
                     126..127,141,159,164,170..171,183..184,204,208,214..215,
                     227..228,245,250,256..257,269..270,288,292)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          19..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          62..97
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          145..182
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          189..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          304..773
                     /region_name="Coatomer_WDAD_beta-like"
                     /note="Coatomer WD Associated Region from Coatomer Subunit
                     Beta and Beta'; cd22947"
                     /db_xref="CDD:438572"
     Region          304..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          353..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          393..425
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          431..462
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          466..514
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Region          518..557
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     Site            order(551,581,700..702,729..730,751..752,755)
                     /site_type="other"
                     /note="coatomer beta'-subunit binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438572"
     Region          558..582
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:438572"
     CDS             1..931
                     /gene="beta'COP"
                     /coded_by="XM_034334372.2:320..3115"
                     /db_xref="GeneID:117608779"
ORIGIN      
        1 mplrldikrk ltarsdrvks vdlhptepwm lcslyqgnvn iwnhetqmlv ktfevcdlpv
       61 rtakfvprkn wvvtgsddmq irvfnyntle rvhsfeahsd yvrciavhpt qpfiltssdd
      121 mliklwnwek awigqqvfeg hthyvmqivf npkdnntfas asldrtvkvw qlgsstanft
      181 leghekgvnc vdyyhggdkp ylisgaddky vkiwdyqnkt cvqtleghtq nisavcfhpe
      241 lpiiltgsed gtvriwhagt yrlesslnyg fervwtiscm rgsnnvaigy degsvmvkvg
      301 reepavsmds lggkivwakh seiqqvnlka lgeeaqdger lplavkdmga ceiypqtiqh
      361 npngrflvvc gdgeyiiyts malrnkafgq asefvwaads sqyavresnt tvkvfknfke
      421 kksfkpdfga dgifggfllg vssgsglsff dwdtlklvrr idiqpthvyw aenaslvtla
      481 ttdqyfilky hgdavvnape nsediedafe mvaemnevvk tglwvgdcfi ytnsvnrvny
      541 fvggeivtvs hldrpmyllg yvprdnrlyl cdkelsvvsy slllsvleyq tavmrkdfet
      601 adrvlptvpk ehrtrvahfl ekqgfkeqal avstdpehrf elalalgdla tahalakean
      661 sqqkwrqlas latqkgklcl aqeclhqaqd fggllllats tgnanmiqkl gtdanetgkn
      721 nisflsyfil gdvdkcldil iktdripeaa ffartyapsr issivklwke klsavsekag
      781 qsladpeqye nlfpgyreal kaeqflreds kkkipasafp nvqsnierkp feemlaaeea
      841 nqftykagpt tnneaevapt evltnrlqdl diskvipsst ekasvpekvt ktnsnsrplt
      901 vddddldldf eldetldttg vsldddllee d
//
LOCUS       XP_034190387            1547 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608843 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190387
VERSION     XP_034190387.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190387.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1547
                     /product="uncharacterized protein LOC117608843 isoform X1"
                     /calculated_mol_wt=173459
     Region          141..447
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Site            order(142,144,147,149,166,169,171,174,176,194,197,199,202,
                     204,223,226,228,231,233,251,254,256,259,261,279,282,284,
                     287,289,307,310,312,315,317,342,345,347,350,352,370,373,
                     375,378,380,398,401,403,406,408,426,429,431,434,436)
                     /site_type="active"
                     /note="Leucine-rich repeats [active]"
                     /db_xref="CDD:238064"
     Site            order(172..173,230,286,314,377,402,404,430)
                     /site_type="other"
                     /note="Substrate binding site [chemical binding]"
                     /db_xref="CDD:238064"
     Region          251..278
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          279..305
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          342..369
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          370..397
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          398..424
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..1547
                     /gene="LOC117608843"
                     /coded_by="XM_034334496.2:211..4854"
                     /db_xref="GeneID:117608843"
ORIGIN      
        1 msgeadsagq icenslttlk nevdpeklpp rtishttrrp rfgignvpls pyilidgkkl
       61 rsslalskrk lprrvsfptn dnllvtgyle panpwklaen vnledlilay kescikhnae
      121 sletvisqle tldiskarne elnlkgqkln inhaetleei fkrvqfnkvd leatflndet
      181 avilfdtiey yesakhlnis snqnigihgw qacanmirkt qclehleakd itlneqymni
      241 lsralplvch lhvlklencg lsgrciitlv salkmntgir elyladnnln lydaiqlgsl
      301 lrlnnhiqll dvsnniiqdd gvrdmlegli nqinedksgk glnilvlwnn qltkksspyf
      361 ariialsktl etlnigknml tdellfvikd alkknrillq lgiqatdltc dgivtlseii
      421 einqilqrid lrnnniqltg mqalcsamqn nksitkidld depntkivdl tqtlhqymql
      481 vaeirscctn neqmhtleen segfqssfhs rfcskisrki sltcqtlpcs lsskisiqkd
      541 esgrsllepk ringgrlrsp ipspssspvv spipspsrsr fvvsrvpets rsadpsvsss
      601 pvlsslgssp tyvtssasgp srfrvsvves anvslpkpvv tstttdhtid infkittdes
      661 idsddldnvf isesktsndq nakslrdtsn ndvsyldsql vnetgtslip ersnvdelna
      721 decfvtatik tksndkicsi tiedkeycdv qnnqntvttq letiipteni envenydded
      781 trnatmscvn inkndtnsnc tivddqsvti dkdnvtekla ntsfdesmql hsqtkhdpym
      841 qksssnlgkl lalfqhpscf fsdstpinql kskntlqgsi nsmmtlgdkf hyyikdkkdk
      901 iynreteasl mslknkstrf fnlsqmpslq sitsmvpsfk fegnlhdsrq snesyskevd
      961 fllkkdydsn ltdtkeecnn eseklnvhlk eqeisvpdld nqlipedirs kypvgiadkn
     1021 iiltancivs nvidtchkiv nsrslkhvsd kkmivpvpks igipeysalk avksdvkkdt
     1081 imetlekvds ilideasnet ltnsinftsd snslscsimy gvskdthtvn ksdrmhslvi
     1141 lnldekdnve nnstfnvnnt ddnqayfvcd ekhgyssntl kgsvernssn nclnnevvem
     1201 hsfdsnkdkn vftdvhnmps neveridvsr vnnsthwkda ivvnstserk isninctckd
     1261 tnfdfnndnn rqlctlilyk dirapvterm iqmfitkktd vnnfimests tvrkgnivmp
     1321 ipktytyvtn inrsvlrdli rhitdising nldkenipsn dwpdnaikvk tsfynqaqis
     1381 yasmnvmset sedlhknnef invendisvn gnveqsmhfh krqfqeceds kidsecsstc
     1441 vkvdsnvhsk rdvpwleknd tqnnssenir llgnfesatt qhlkctnsdd tnetahkyfn
     1501 aikcavattm cststvntas nktikiisdq snkiaesssv ihatstk
//
LOCUS       XP_076545603            1452 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608843 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545603
VERSION     XP_076545603.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689488.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1452
                     /product="uncharacterized protein LOC117608843 isoform X2"
                     /calculated_mol_wt=162951
     Region          46..352
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Site            order(47,49,52,54,71,74,76,79,81,99,102,104,107,109,128,
                     131,133,136,138,156,159,161,164,166,184,187,189,192,194,
                     212,215,217,220,222,247,250,252,255,257,275,278,280,283,
                     285,303,306,308,311,313,331,334,336,339,341)
                     /site_type="active"
                     /note="Leucine-rich repeats [active]"
                     /db_xref="CDD:238064"
     Site            order(77..78,135,191,219,282,307,309,335)
                     /site_type="other"
                     /note="Substrate binding site [chemical binding]"
                     /db_xref="CDD:238064"
     Region          156..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..210
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          247..274
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          275..302
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          303..329
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..1452
                     /gene="LOC117608843"
                     /coded_by="XM_076689488.1:77..4435"
                     /db_xref="GeneID:117608843"
ORIGIN      
        1 mqaenvnled lilaykesci khnaesletv isqletldis karneelnlk gqklninhae
       61 tleeifkrvq fnkvdleatf lndetavilf dtieyyesak hlnissnqni gihgwqacan
      121 mirktqcleh leakditlne qymnilsral plvchlhvlk lencglsgrc iitlvsalkm
      181 ntgirelyla dnnlnlydai qlgsllrlnn hiqlldvsnn iiqddgvrdm leglinqine
      241 dksgkglnil vlwnnqltkk sspyfariia lsktletlni gknmltdell fvikdalkkn
      301 rillqlgiqa tdltcdgivt lseiieinqi lqridlrnnn iqltgmqalc samqnnksit
      361 kidlddepnt kivdltqtlh qymqlvaeir scctnneqmh tleensegfq ssfhsrfcsk
      421 isrkisltcq tlpcslsski siqkdesgrs llepkringg rlrspipsps sspvvspips
      481 psrsrfvvsr vpetsrsadp svssspvlss lgssptyvts sasgpsrfrv svvesanvsl
      541 pkpvvtsttt dhtidinfki ttdesidsdd ldnvfisesk tsndqnaksl rdtsnndvsy
      601 ldsqlvnetg tslipersnv delnadecfv tatiktksnd kicsitiedk eycdvqnnqn
      661 tvttqletii ptenienven yddedtrnat mscvninknd tnsnctivdd qsvtidkdnv
      721 teklantsfd esmqlhsqtk hdpymqksss nlgkllalfq hpscffsdst pinqlksknt
      781 lqgsinsmmt lgdkfhyyik dkkdkiynre teaslmslkn kstrffnlsq mpslqsitsm
      841 vpsfkfegnl hdsrqsnesy skevdfllkk dydsnltdtk eecnnesekl nvhlkeqeis
      901 vpdldnqlip edirskypvg iadkniilta ncivsnvidt chkivnsrsl khvsdkkmiv
      961 pvpksigipe ysalkavksd vkkdtimetl ekvdsilide asnetltnsi nftsdsnsls
     1021 csimygvskd thtvnksdrm hslvilnlde kdnvennstf nvnntddnqa yfvcdekhgy
     1081 ssntlkgsve rnssnnclnn evvemhsfds nkdknvftdv hnmpsnever idvsrvnnst
     1141 hwkdaivvns tserkisnin ctckdtnfdf nndnnrqlct lilykdirap vtermiqmfi
     1201 tkktdvnnfi meststvrkg nivmpipkty tyvtninrsv lrdlirhitd isingnldke
     1261 nipsndwpdn aikvktsfyn qaqisyasmn vmsetsedlh knnefinven disvngnveq
     1321 smhfhkrqfq ecedskidse csstcvkvds nvhskrdvpw lekndtqnns senirllgnf
     1381 esattqhlkc tnsddtneta hkyfnaikca vattmcstst vntasnktik iisdqsnkia
     1441 esssvihats tk
//
LOCUS       XP_076545604             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305505 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545604
VERSION     XP_076545604.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689489.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC143305505 isoform X1"
                     /calculated_mol_wt=82847
     CDS             1..729
                     /gene="LOC143305505"
                     /coded_by="XM_076689489.1:1..2190"
                     /db_xref="GeneID:143305505"
ORIGIN      
        1 menrkqmvpg ennsdgkedd dvsviaiiek endrslrheq tektdekqen rrviedkkyk
       61 edsdyvrdst etssgastsg ggvskirrvy ravrkthsvt evrrnpirak kkdpdknfpr
      121 lrqrllaakk kemlfssedg krgksstkpy mntrsvtrkm ynvgatyqap tmkdalewke
      181 wpvhgmherp vfhpqlglaa eylgryftsf dglsyceiin rpeievvsvd phcdslhssf
      241 ekkrskksks srsftnirns kdpsekvksf escmhesfhc vlgycsqvmt ptyktnvegk
      301 ldmlnekkns stvvketpkq sniveqtprk dspnnskenl qkpieenrmd nytavmmpqn
      361 aknfnqipkt qlfpaqkvyl ansqksiafa nmktlqggqv kiqdavrpsk npfilvntsd
      421 ikdtrfvkst pstvntkaed tivgeenlge lssiykhfdl seqfgskkap tkrekkvefa
      481 mtryllntak htkiqipnag egklyyeipc kglkvkspst tlnktcysne ateiakilse
      541 ynknttkkpa senasfcpgk ntrvnelsck dentsqnsqe tciekntqlt fshgkwrrfh
      601 ltmgkardlk sirneekllp nrqslfrinp tglrsspggm ievskhlell qrrqtnvenv
      661 eekkrasats ddvdstapkm qylqelledt amlycaatgt hqddlanyid tldavqsvqw
      721 lescknsit
//
LOCUS       XP_076545605             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305505 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545605
VERSION     XP_076545605.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689490.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC143305505 isoform X1"
                     /calculated_mol_wt=82847
     CDS             1..729
                     /gene="LOC143305505"
                     /coded_by="XM_076689490.1:1..2190"
                     /db_xref="GeneID:143305505"
ORIGIN      
        1 menrkqmvpg ennsdgkedd dvsviaiiek endrslrheq tektdekqen rrviedkkyk
       61 edsdyvrdst etssgastsg ggvskirrvy ravrkthsvt evrrnpirak kkdpdknfpr
      121 lrqrllaakk kemlfssedg krgksstkpy mntrsvtrkm ynvgatyqap tmkdalewke
      181 wpvhgmherp vfhpqlglaa eylgryftsf dglsyceiin rpeievvsvd phcdslhssf
      241 ekkrskksks srsftnirns kdpsekvksf escmhesfhc vlgycsqvmt ptyktnvegk
      301 ldmlnekkns stvvketpkq sniveqtprk dspnnskenl qkpieenrmd nytavmmpqn
      361 aknfnqipkt qlfpaqkvyl ansqksiafa nmktlqggqv kiqdavrpsk npfilvntsd
      421 ikdtrfvkst pstvntkaed tivgeenlge lssiykhfdl seqfgskkap tkrekkvefa
      481 mtryllntak htkiqipnag egklyyeipc kglkvkspst tlnktcysne ateiakilse
      541 ynknttkkpa senasfcpgk ntrvnelsck dentsqnsqe tciekntqlt fshgkwrrfh
      601 ltmgkardlk sirneekllp nrqslfrinp tglrsspggm ievskhlell qrrqtnvenv
      661 eekkrasats ddvdstapkm qylqelledt amlycaatgt hqddlanyid tldavqsvqw
      721 lescknsit
//
LOCUS       XP_076545607             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305505 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545607
VERSION     XP_076545607.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689492.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC143305505 isoform X1"
                     /calculated_mol_wt=82847
     CDS             1..729
                     /gene="LOC143305505"
                     /coded_by="XM_076689492.1:1..2190"
                     /db_xref="GeneID:143305505"
ORIGIN      
        1 menrkqmvpg ennsdgkedd dvsviaiiek endrslrheq tektdekqen rrviedkkyk
       61 edsdyvrdst etssgastsg ggvskirrvy ravrkthsvt evrrnpirak kkdpdknfpr
      121 lrqrllaakk kemlfssedg krgksstkpy mntrsvtrkm ynvgatyqap tmkdalewke
      181 wpvhgmherp vfhpqlglaa eylgryftsf dglsyceiin rpeievvsvd phcdslhssf
      241 ekkrskksks srsftnirns kdpsekvksf escmhesfhc vlgycsqvmt ptyktnvegk
      301 ldmlnekkns stvvketpkq sniveqtprk dspnnskenl qkpieenrmd nytavmmpqn
      361 aknfnqipkt qlfpaqkvyl ansqksiafa nmktlqggqv kiqdavrpsk npfilvntsd
      421 ikdtrfvkst pstvntkaed tivgeenlge lssiykhfdl seqfgskkap tkrekkvefa
      481 mtryllntak htkiqipnag egklyyeipc kglkvkspst tlnktcysne ateiakilse
      541 ynknttkkpa senasfcpgk ntrvnelsck dentsqnsqe tciekntqlt fshgkwrrfh
      601 ltmgkardlk sirneekllp nrqslfrinp tglrsspggm ievskhlell qrrqtnvenv
      661 eekkrasats ddvdstapkm qylqelledt amlycaatgt hqddlanyid tldavqsvqw
      721 lescknsit
//
LOCUS       XP_076545608             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305505 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545608
VERSION     XP_076545608.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689493.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC143305505 isoform X1"
                     /calculated_mol_wt=82847
     CDS             1..729
                     /gene="LOC143305505"
                     /coded_by="XM_076689493.1:1..2190"
                     /db_xref="GeneID:143305505"
ORIGIN      
        1 menrkqmvpg ennsdgkedd dvsviaiiek endrslrheq tektdekqen rrviedkkyk
       61 edsdyvrdst etssgastsg ggvskirrvy ravrkthsvt evrrnpirak kkdpdknfpr
      121 lrqrllaakk kemlfssedg krgksstkpy mntrsvtrkm ynvgatyqap tmkdalewke
      181 wpvhgmherp vfhpqlglaa eylgryftsf dglsyceiin rpeievvsvd phcdslhssf
      241 ekkrskksks srsftnirns kdpsekvksf escmhesfhc vlgycsqvmt ptyktnvegk
      301 ldmlnekkns stvvketpkq sniveqtprk dspnnskenl qkpieenrmd nytavmmpqn
      361 aknfnqipkt qlfpaqkvyl ansqksiafa nmktlqggqv kiqdavrpsk npfilvntsd
      421 ikdtrfvkst pstvntkaed tivgeenlge lssiykhfdl seqfgskkap tkrekkvefa
      481 mtryllntak htkiqipnag egklyyeipc kglkvkspst tlnktcysne ateiakilse
      541 ynknttkkpa senasfcpgk ntrvnelsck dentsqnsqe tciekntqlt fshgkwrrfh
      601 ltmgkardlk sirneekllp nrqslfrinp tglrsspggm ievskhlell qrrqtnvenv
      661 eekkrasats ddvdstapkm qylqelledt amlycaatgt hqddlanyid tldavqsvqw
      721 lescknsit
//
LOCUS       XP_076545609             705 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305505 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545609
VERSION     XP_076545609.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689494.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..705
                     /product="uncharacterized protein LOC143305505 isoform X2"
                     /calculated_mol_wt=80075
     CDS             1..705
                     /gene="LOC143305505"
                     /coded_by="XM_076689494.1:1..2118"
                     /db_xref="GeneID:143305505"
ORIGIN      
        1 menrkqmvpg ennsdgkedd dvsviaiiek endrslrheq tektdekqen rrviedkkyk
       61 edsdyvrdst etssgastsg ggvskirrvy ravrkthsvt evrrnpirak kkdpdknfpr
      121 lrqrllaakk kemlfssedg atyqaptmkd alewkewpvh gmherpvfhp qlglaaeylg
      181 ryftsfdgls yceiinrpei evvsvdphcd slhssfekkr skkskssrsf tnirnskdps
      241 ekvksfescm hesfhcvlgy csqvmtptyk tnvegkldml nekknsstvv ketpkqsniv
      301 eqtprkdspn nskenlqkpi eenrmdnyta vmmpqnaknf nqipktqlfp aqkvylansq
      361 ksiafanmkt lqggqvkiqd avrpsknpfi lvntsdikdt rfvkstpstv ntkaedtivg
      421 eenlgelssi ykhfdlseqf gskkaptkre kkvefamtry llntakhtki qipnagegkl
      481 yyeipckglk vkspsttlnk tcysneatei akilseynkn ttkkpasena sfcpgkntrv
      541 nelsckdent sqnsqetcie kntqltfshg kwrrfhltmg kardlksirn eekllpnrqs
      601 lfrinptglr sspggmievs khlellqrrq tnvenveekk rasatsddvd stapkmqylq
      661 elledtamly caatgthqdd lanyidtlda vqsvqwlesc knsit
//
LOCUS       XP_076545610             597 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305505 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545610
VERSION     XP_076545610.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689495.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="uncharacterized protein LOC143305505 isoform X3"
                     /calculated_mol_wt=67550
     CDS             1..597
                     /gene="LOC143305505"
                     /coded_by="XM_076689495.1:245..2038"
                     /db_xref="GeneID:143305505"
ORIGIN      
        1 mlfssedgkr gksstkpymn trsvtrkmyn vgatyqaptm kdalewkewp vhgmherpvf
       61 hpqlglaaey lgryftsfdg lsyceiinrp eievvsvdph cdslhssfek krskkskssr
      121 sftnirnskd psekvksfes cmhesfhcvl gycsqvmtpt yktnvegkld mlnekknsst
      181 vvketpkqsn iveqtprkds pnnskenlqk pieenrmdny tavmmpqnak nfnqipktql
      241 fpaqkvylan sqksiafanm ktlqggqvki qdavrpsknp filvntsdik dtrfvkstps
      301 tvntkaedti vgeenlgels siykhfdlse qfgskkaptk rekkvefamt ryllntakht
      361 kiqipnageg klyyeipckg lkvkspsttl nktcysneat eiakilseyn knttkkpase
      421 nasfcpgknt rvnelsckde ntsqnsqetc iekntqltfs hgkwrrfhlt mgkardlksi
      481 rneekllpnr qslfrinptg lrsspggmie vskhlellqr rqtnvenvee kkrasatsdd
      541 vdstapkmqy lqelledtam lycaatgthq ddlanyidtl davqsvqwle scknsit
//
LOCUS       XP_034184888             489 aa            linear   INV 03-SEP-2025
DEFINITION  tetratricopeptide repeat protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184888
VERSION     XP_034184888.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328997.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="tetratricopeptide repeat protein 2 isoform X1"
                     /calculated_mol_wt=56088
     Region          19..47
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(20,23..24,27..28,30,54,57..58,61..62,64..65,88,
                     91..92,95..96,99)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          33..277
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          52..82
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          87..115
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          175..389
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          201..229
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(252,255..256,258,286,289..290,293..294,296..297,320,
                     323..324,327..328,331)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          252..275
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          284..314
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          319..347
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          372..>484
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..489
                     /gene="Tpr2"
                     /coded_by="XM_034328997.2:242..1711"
                     /db_xref="GeneID:117606496"
ORIGIN      
        1 matammsdmd peksveevan kknqaakely fvkeykkalv eyselielcp nnavyygnra
       61 acnmmlakyr daladakksi eldpklfkay vrvvkcslil gdimeaenil tklteldpen
      121 eaipvekrvl ktvqkfikda esayaakdyr kvvycmdrcl dvstsctrfk ltkseclafl
      181 gryqeaqkiv ndilhfdkqn vdamciraic lyfqdnidka fahfqqvlrl apdhvkalei
      241 ykkakslkkk keegnaaykk eqyqeaykly sealaidphn ivtnaklhfn kamaaaklrr
      301 lnesvvecse alkldenylk allrraasym eldefekavh dlekackldk srenkrllme
      361 aklalkkslr kdyykilgid knastddikk ayrkramvhh pdrhpnateg ekkeqekkfk
      421 evgeaygils dpkkrsryds ghdiydiddg dggfhdidps amfqtffnqh dgyqfhtsgf
      481 pggftfhfg
//
LOCUS       XP_034184889             483 aa            linear   INV 03-SEP-2025
DEFINITION  tetratricopeptide repeat protein 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184889
VERSION     XP_034184889.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328998.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="tetratricopeptide repeat protein 2 isoform X2"
                     /calculated_mol_wt=55517
     Region          19..47
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(20,23..24,27..28,30,54,57..58,61..62,64..65,88,
                     91..92,95..96,99)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          33..277
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          52..82
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          87..115
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          175..389
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          201..229
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(252,255..256,258,286,289..290,293..294,296..297,320,
                     323..324,327..328,331)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          252..275
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          284..314
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          319..347
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          372..>478
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     CDS             1..483
                     /gene="Tpr2"
                     /coded_by="XM_034328998.2:242..1693"
                     /db_xref="GeneID:117606496"
ORIGIN      
        1 matammsdmd peksveevan kknqaakely fvkeykkalv eyselielcp nnavyygnra
       61 acnmmlakyr daladakksi eldpklfkay vrvvkcslil gdimeaenil tklteldpen
      121 eaipvekrvl ktvqkfikda esayaakdyr kvvycmdrcl dvstsctrfk ltkseclafl
      181 gryqeaqkiv ndilhfdkqn vdamciraic lyfqdnidka fahfqqvlrl apdhvkalei
      241 ykkakslkkk keegnaaykk eqyqeaykly sealaidphn ivtnaklhfn kamaaaklrr
      301 lnesvvecse alkldenylk allrraasym eldefekavh dlekackldk srenkrllme
      361 aklalkkslr kdyykilgid knastddikk ayrkramvhh pdrhpnateg ekkeqekkfk
      421 evgeaygils dpkkrsryds ghdiydiddd idpsamfqtf fnqhdgyqfh tsgfpggftf
      481 hfg
//
LOCUS       XP_076545611             230 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606626 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545611
VERSION     XP_076545611.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689496.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="uncharacterized protein LOC117606626 isoform X1"
                     /calculated_mol_wt=26015
     Region          107..167
                     /region_name="bHLH_TS_ceHLH13_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Caenorhabditis elegans Helix-loop-helix protein 13 (HLH13)
                     and similar proteins; cd11416"
                     /db_xref="CDD:381422"
     Site            order(112,116,118..120,122,128,146..149)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381422"
     Site            order(125,127,130..131,133..134,150,153,157,159..160,164,
                     166..167)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381422"
     CDS             1..230
                     /gene="LOC117606626"
                     /coded_by="XM_076689496.1:3574..4266"
                     /db_xref="GeneID:117606626"
ORIGIN      
        1 mashstyeye drgqrmrhrv gttsraadst vactssqyyv gpssdiseed lvelpscvya
       61 grssqhvpht sshthsplhy hmpvstpdhn dtelngveeg yypngspyri qrhaanirer
      121 krmlssinsa fdelrvhvpt fpyekrlski dtlrlaiayi allrevlaar ldpltyverc
      181 lrgeingera ewntsdltar lswinwenlg vnpnrrsvlt tlalttdnmn
//
LOCUS       XP_076545612             230 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606626 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545612
VERSION     XP_076545612.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689497.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="uncharacterized protein LOC117606626 isoform X1"
                     /calculated_mol_wt=26015
     Region          107..167
                     /region_name="bHLH_TS_ceHLH13_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Caenorhabditis elegans Helix-loop-helix protein 13 (HLH13)
                     and similar proteins; cd11416"
                     /db_xref="CDD:381422"
     Site            order(112,116,118..120,122,128,146..149)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381422"
     Site            order(125,127,130..131,133..134,150,153,157,159..160,164,
                     166..167)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381422"
     CDS             1..230
                     /gene="LOC117606626"
                     /coded_by="XM_076689497.1:2908..3600"
                     /db_xref="GeneID:117606626"
ORIGIN      
        1 mashstyeye drgqrmrhrv gttsraadst vactssqyyv gpssdiseed lvelpscvya
       61 grssqhvpht sshthsplhy hmpvstpdhn dtelngveeg yypngspyri qrhaanirer
      121 krmlssinsa fdelrvhvpt fpyekrlski dtlrlaiayi allrevlaar ldpltyverc
      181 lrgeingera ewntsdltar lswinwenlg vnpnrrsvlt tlalttdnmn
//
LOCUS       XP_076545613             230 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606626 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545613
VERSION     XP_076545613.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689498.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="uncharacterized protein LOC117606626 isoform X1"
                     /calculated_mol_wt=26015
     Region          107..167
                     /region_name="bHLH_TS_ceHLH13_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Caenorhabditis elegans Helix-loop-helix protein 13 (HLH13)
                     and similar proteins; cd11416"
                     /db_xref="CDD:381422"
     Site            order(112,116,118..120,122,128,146..149)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381422"
     Site            order(125,127,130..131,133..134,150,153,157,159..160,164,
                     166..167)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381422"
     CDS             1..230
                     /gene="LOC117606626"
                     /coded_by="XM_076689498.1:1730..2422"
                     /db_xref="GeneID:117606626"
ORIGIN      
        1 mashstyeye drgqrmrhrv gttsraadst vactssqyyv gpssdiseed lvelpscvya
       61 grssqhvpht sshthsplhy hmpvstpdhn dtelngveeg yypngspyri qrhaanirer
      121 krmlssinsa fdelrvhvpt fpyekrlski dtlrlaiayi allrevlaar ldpltyverc
      181 lrgeingera ewntsdltar lswinwenlg vnpnrrsvlt tlalttdnmn
//
LOCUS       XP_076545614             229 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606626 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545614
VERSION     XP_076545614.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689499.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..229
                     /product="uncharacterized protein LOC117606626 isoform X2"
                     /calculated_mol_wt=25928
     Region          106..166
                     /region_name="bHLH_TS_ceHLH13_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Caenorhabditis elegans Helix-loop-helix protein 13 (HLH13)
                     and similar proteins; cd11416"
                     /db_xref="CDD:381422"
     Site            order(111,115,117..119,121,127,145..148)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381422"
     Site            order(124,126,129..130,132..133,149,152,156,158..159,163,
                     165..166)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381422"
     CDS             1..229
                     /gene="LOC117606626"
                     /coded_by="XM_076689499.1:3048..3737"
                     /db_xref="GeneID:117606626"
ORIGIN      
        1 mashstyeye drgqrmrhrv gttsraadst vactsqyyvg pssdiseedl velpscvyag
       61 rssqhvphts shthsplhyh mpvstpdhnd telngveegy ypngspyriq rhaanirerk
      121 rmlssinsaf delrvhvptf pyekrlskid tlrlaiayia llrevlaarl dpltyvercl
      181 rgeingerae wntsdltarl swinwenlgv npnrrsvltt lalttdnmn
//
LOCUS       XP_076545615             229 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606626 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545615
VERSION     XP_076545615.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689500.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..229
                     /product="uncharacterized protein LOC117606626 isoform X3"
                     /calculated_mol_wt=25928
     Region          107..166
                     /region_name="bHLH_TS_ceHLH13_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Caenorhabditis elegans Helix-loop-helix protein 13 (HLH13)
                     and similar proteins; cd11416"
                     /db_xref="CDD:381422"
     Site            order(112,116,118..120,122,127,145..148)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381422"
     Site            order(125..126,129..130,132..133,149,152,156,158..159,163,
                     165..166)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381422"
     CDS             1..229
                     /gene="LOC117606626"
                     /coded_by="XM_076689500.1:3048..3737"
                     /db_xref="GeneID:117606626"
ORIGIN      
        1 mashstyeye drgqrmrhrv gttsraadst vactssqyyv gpssdiseed lvelpscvya
       61 grssqhvpht sshthsplhy hmpvstpdhn dtelngveeg yypngspyri qrhaanirer
      121 krmlsinsaf delrvhvptf pyekrlskid tlrlaiayia llrevlaarl dpltyvercl
      181 rgeingerae wntsdltarl swinwenlgv npnrrsvltt lalttdnmn
//
LOCUS       XP_034190616             399 aa            linear   INV 03-SEP-2025
DEFINITION  farnesyl pyrophosphate synthase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190616
VERSION     XP_034190616.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334725.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190616.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="farnesyl pyrophosphate synthase-like isoform X1"
                     /calculated_mol_wt=46073
     Region          85..350
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..399
                     /gene="LOC117608947"
                     /coded_by="XM_034334725.2:139..1338"
                     /db_xref="GeneID:117608947"
ORIGIN      
        1 mniyrackva rttysaldsy rfneslffem nsksnsadvr tmysvtestf lsnkdesrel
       61 maiwpdvvrd ltdtgrhldi pdvtkwmakv lqynvpegrk nralsvvyaf ksfasvdqlt
      121 eenirlsril gwciellqaf llviddiqdq stmrrnapcw ylyndiglaa indgmmlemc
      181 vyqllrkhfr skdcyvslme lfldtslkts mgqcldllst nfgkksnldl ftmdrynsiv
      241 kyktayytfv lpttmamcfa gikdpemcrk aktillemgh ffqvqddymn cygsteatgk
      301 tgsdieegkc swlvvvalqr vtpeqrkilk dcygqsdiek snrvkqlyde ldlsntyaiy
      361 eeetynllmt hiqqiscglp hdyflnller acprvarkd
//
LOCUS       XP_034190615             395 aa            linear   INV 03-SEP-2025
DEFINITION  farnesyl pyrophosphate synthase-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190615
VERSION     XP_034190615.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334724.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190615.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..395
                     /product="farnesyl pyrophosphate synthase-like isoform X2"
                     /calculated_mol_wt=45474
     Region          81..346
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..395
                     /gene="LOC117608947"
                     /coded_by="XM_034334724.2:277..1464"
                     /db_xref="GeneID:117608947"
ORIGIN      
        1 msvvvveqrr shtnayvvtd thgwpefnvs nsadvrtmys vtestflsnk desrelmaiw
       61 pdvvrdltdt grhldipdvt kwmakvlqyn vpegrknral svvyafksfa svdqlteeni
      121 rlsrilgwci ellqafllvi ddiqdqstmr rnapcwylyn diglaaindg mmlemcvyql
      181 lrkhfrskdc yvslmelfld tslktsmgqc ldllstnfgk ksnldlftmd rynsivkykt
      241 ayytfvlptt mamcfagikd pemcrkakti llemghffqv qddymncygs teatgktgsd
      301 ieegkcswlv vvalqrvtpe qrkilkdcyg qsdieksnrv kqlydeldls ntyaiyeeet
      361 ynllmthiqq iscglphdyf lnlleracpr varkd
//
LOCUS       XP_034190617             374 aa            linear   INV 03-SEP-2025
DEFINITION  farnesyl pyrophosphate synthase-like isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190617
VERSION     XP_034190617.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334726.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190617.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..374
                     /product="farnesyl pyrophosphate synthase-like isoform X3"
                     /calculated_mol_wt=43195
     Region          60..325
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..374
                     /gene="LOC117608947"
                     /coded_by="XM_034334726.2:460..1584"
                     /db_xref="GeneID:117608947"
ORIGIN      
        1 mfkfhqnqsn sadvrtmysv testflsnkd esrelmaiwp dvvrdltdtg rhldipdvtk
       61 wmakvlqynv pegrknrals vvyafksfas vdqlteenir lsrilgwcie llqafllvid
      121 diqdqstmrr napcwylynd iglaaindgm mlemcvyqll rkhfrskdcy vslmelfldt
      181 slktsmgqcl dllstnfgkk snldlftmdr ynsivkykta yytfvlpttm amcfagikdp
      241 emcrkaktil lemghffqvq ddymncygst eatgktgsdi eegkcswlvv valqrvtpeq
      301 rkilkdcygq sdieksnrvk qlydeldlsn tyaiyeeety nllmthiqqi scglphdyfl
      361 nlleracprv arkd
//
LOCUS       XP_034190620             358 aa            linear   INV 03-SEP-2025
DEFINITION  farnesyl pyrophosphate synthase-like isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190620
VERSION     XP_034190620.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190620.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="farnesyl pyrophosphate synthase-like isoform X4"
                     /calculated_mol_wt=41303
     Region          44..309
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..358
                     /gene="LOC117608947"
                     /coded_by="XM_034334729.2:163..1239"
                     /db_xref="GeneID:117608947"
ORIGIN      
        1 mysvtestfl snkdesrelm aiwpdvvrdl tdtgrhldip dvtkwmakvl qynvpegrkn
       61 ralsvvyafk sfasvdqlte enirlsrilg wciellqafl lviddiqdqs tmrrnapcwy
      121 lyndiglaai ndgmmlemcv yqllrkhfrs kdcyvslmel fldtslktsm gqcldllstn
      181 fgkksnldlf tmdrynsivk yktayytfvl pttmamcfag ikdpemcrka ktillemghf
      241 fqvqddymnc ygsteatgkt gsdieegkcs wlvvvalqrv tpeqrkilkd cygqsdieks
      301 nrvkqlydel dlsntyaiye eetynllmth iqqiscglph dyflnllera cprvarkd
//
LOCUS       XP_034190621             358 aa            linear   INV 03-SEP-2025
DEFINITION  farnesyl pyrophosphate synthase-like isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190621
VERSION     XP_034190621.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334730.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190621.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="farnesyl pyrophosphate synthase-like isoform X4"
                     /calculated_mol_wt=41303
     Region          44..309
                     /region_name="polyprenyl_synt"
                     /note="Polyprenyl synthetase; pfam00348"
                     /db_xref="CDD:459773"
     CDS             1..358
                     /gene="LOC117608947"
                     /coded_by="XM_034334730.2:202..1278"
                     /db_xref="GeneID:117608947"
ORIGIN      
        1 mysvtestfl snkdesrelm aiwpdvvrdl tdtgrhldip dvtkwmakvl qynvpegrkn
       61 ralsvvyafk sfasvdqlte enirlsrilg wciellqafl lviddiqdqs tmrrnapcwy
      121 lyndiglaai ndgmmlemcv yqllrkhfrs kdcyvslmel fldtslktsm gqcldllstn
      181 fgkksnldlf tmdrynsivk yktayytfvl pttmamcfag ikdpemcrka ktillemghf
      241 fqvqddymnc ygsteatgkt gsdieegkcs wlvvvalqrv tpeqrkilkd cygqsdieks
      301 nrvkqlydel dlsntyaiye eetynllmth iqqiscglph dyflnllera cprvarkd
//
LOCUS       XP_076545616             294 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic phosphatidylinositol transfer protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545616
VERSION     XP_076545616.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689501.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="cytoplasmic phosphatidylinositol transfer
                     protein 1"
                     /calculated_mol_wt=34574
     Region          3..251
                     /region_name="SRPBCC"
                     /note="START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC (SRPBCC)
                     ligand-binding domain superfamily; cl14643"
                     /db_xref="CDD:472699"
     Site            order(5,7,9,18..20,22..23,40,59,61,63,65,85,87,89,94,96,
                     109..110,112,114,185,187,189,191,202..206,208..209,
                     212..214,217)
                     /site_type="active"
                     /note="hydrophobic ligand binding site [active]"
                     /db_xref="CDD:176899"
     CDS             1..294
                     /gene="rdgBbeta"
                     /coded_by="XM_076689501.1:477..1361"
                     /db_xref="GeneID:117608949"
ORIGIN      
        1 mvltkeyric mpltteeyhi gqlymiarhs heqsdndegv evvenvecdh pehgkgqyte
       61 krihlssklp ywiqsvlpri fyvtekawny ypftiteytc sfipkfhisi rtrykddngc
      121 tenclglspi evlhrevdyl diaydeisak hykeeedpkf fqskrtgrgp lvegwrettq
      181 pimcsyklih asfevwgmqt rvedfihrci rdilllghrq afawidewyd mtledvrrfe
      241 qkmqaetnek vrlrnmtsek psapttptss ipssplpksp tsptqsnrsw fsws
//
LOCUS       XP_034194888             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194888
VERSION     XP_034194888.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338997.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_034338997.2:1570..2628"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_034194889             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194889
VERSION     XP_034194889.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338998.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_034338998.2:1753..2811"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_076545618             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545618
VERSION     XP_076545618.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689503.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_076689503.1:1800..2858"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_034194890             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194890
VERSION     XP_034194890.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338999.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_034338999.2:1849..2907"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_034194891             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194891
VERSION     XP_034194891.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339000.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_034339000.2:847..1905"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_076545619             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545619
VERSION     XP_076545619.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689504.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_076689504.1:701..1759"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_034194896             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194896
VERSION     XP_034194896.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339005.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_034339005.2:1362..2420"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_034194897             352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194897
VERSION     XP_034194897.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339006.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="uncharacterized protein LOC117611100 isoform X1"
                     /calculated_mol_wt=38141
     CDS             1..352
                     /gene="LOC117611100"
                     /coded_by="XM_034339006.2:1251..2309"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 msegktksvl pmpvvtstqc cnnltdtqcp glqvtsrmlr spshesvttd lslfsvssia
       61 selrgvnrlv tfnsytdvhl agrgpspkpn srqiqtnrpa nipeilwfee enelirscgv
      121 lssalglrks fstsdvsqlp spdasgpsgl raamsalald taqrntllle htrldhtsrs
      181 cstwvaigep vantsqlpsp hggaaqeqqn qqqssnsrsv pqpppapppp apftgadfvr
      241 svnkkvrqny irrrllttyr alerlsqsef nldqlevvas aaqttsgttl lvpgataatg
      301 ssipgrkern haltikdver ergnqlskye rnmmifnwlh tlddtaavds ve
//
LOCUS       XP_034194901             315 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194901
VERSION     XP_034194901.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339010.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="uncharacterized protein LOC117611100 isoform X2"
                     /calculated_mol_wt=34203
     CDS             1..315
                     /gene="LOC117611100"
                     /coded_by="XM_034339010.2:1589..2536"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 mlrspshesv ttdlslfsvs siaselrgvn rlvtfnsytd vhlagrgpsp kpnsrqiqtn
       61 rpanipeilw feeenelirs cgvlssalgl rksfstsdvs qlpspdasgp sglraamsal
      121 aldtaqrntl llehtrldht srscstwvai gepvantsql psphggaaqe qqnqqqssns
      181 rsvpqpppap pppapftgad fvrsvnkkvr qnyirrrllt tyralerlsq sefnldqlev
      241 vasaaqttsg ttllvpgata atgssipgrk ernhaltikd verergnqls kyernmmifn
      301 wlhtlddtaa vdsve
//
LOCUS       XP_034194902             307 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611100 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194902
VERSION     XP_034194902.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339011.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="uncharacterized protein LOC117611100 isoform X3"
                     /calculated_mol_wt=33417
     CDS             1..307
                     /gene="LOC117611100"
                     /coded_by="XM_034339011.2:1022..1945"
                     /db_xref="GeneID:117611100"
ORIGIN      
        1 mlqkrhhlgt vssiaselrg vnrlvtfnsy tdvhlagrgp spkpnsrqiq tnrpanipei
       61 lwfeeeneli rscgvlssal glrksfstsd vsqlpspdas gpsglraams alaldtaqrn
      121 tlllehtrld htsrscstwv aigepvants qlpsphggaa qeqqnqqqss nsrsvpqppp
      181 appppapftg adfvrsvnkk vrqnyirrrl lttyralerl sqsefnldql evvasaaqtt
      241 sgttllvpga taatgssipg rkernhalti kdverergnq lskyernmmi fnwlhtlddt
      301 aavdsve
//
LOCUS       XP_034185287            3905 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185287
VERSION     XP_034185287.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329396.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3905
                     /product="terribly reduced optic lobes isoform X1"
                     /calculated_mol_wt=430596
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          800..834
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(805,813,824..825)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(817,820,824,830..831)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            827..831
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          838..872
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(843,851,862..863)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(855,858,862,868..869)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            865..869
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          874..908
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(879,887,898..899)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(891,894,898,904..905)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            901..905
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          922..956
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(927,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          960..991
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(965,973,984..985)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(977,980,984,990..991)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            987..991
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1007..1042
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1012,1021,1032..1033)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1025,1028,1032,1038..1039)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1035..1039
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1067..1143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1073..1077
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1086..1090
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1106..1110
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1157..1191
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1162,1170,1181..1182)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1174,1177,1181,1187..1188)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1184..1188
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1196..1230
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1201,1209,1220..1221)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1213,1216,1220,1226..1227)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1223..1227
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1239..1274
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1244,1253,1264..1265)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1257,1260,1264,1270..1271)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1267..1271
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1292..1367
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1295..1299
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1308..1312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1331..1335
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1345..1350
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1359..1362
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1469..1603
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1659..1712
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1660,1662,1675,1685,1687,1696)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1719..1765
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1720,1730,1737,1739,1748)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1836..1972
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2007..2055
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2007,2009,2016,2023,2026,2035)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2062..2108
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2062,2064,2074,2079,2081,2090)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2178..2314
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2510..2591
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2521..2525
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2536..2540
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2557..2561
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2605..2682
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2616..2620
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2629..2633
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2648..2652
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2662..2667
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2675..2678
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2700..2779
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2711..2715
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2724..2728
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2744..2748
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2758..2763
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2771..2774
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2792..2870
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2803..2807
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2816..2820
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2835..2840
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2850..2855
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2863..2866
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2887..2963
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2894..2898
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2908..2912
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2929..2933
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2943..2948
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2956..2959
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2978..3061
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2986..2990
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2999..3003
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3023..3031
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3041..3046
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3054..3057
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3085..3164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3101..3105
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3115..3120
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3136..3140
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3150..3155
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3158..3161
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3176..3259
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3193..3197
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3206..3210
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3225..3229
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3239..3244
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3252..3255
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3349
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3285..3289
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3297..3301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3315..3319
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3329..3334
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3343..3346
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3429..3502
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3435..3439
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3448..3452
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3468..3472
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3482..3487
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3495..3498
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3520..3589
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3523..3527
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3536..3540
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3556..3559
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3569..3574
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3582..3585
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3595..3747
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3770..3803
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3905
                     /gene="trol"
                     /coded_by="XM_034329396.2:1530..13247"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd eagcvnecpa
      841 gmfrcndglc ldskrrcdgr phcldgsdei ncqcpdgelp cdngvcinkn ffcdrnadch
      901 dgsderdcpv idettdgdhv acrqdefacp hggcipqsav cngrndcpye edeincprgc
      961 gkdqfqcsng dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag yimcvsdkdc
     1021 vpqssmcngi pecrdrsdee nceqlkepsr lnlktypseq vikenpakqg revvfqcrde
     1081 gplrarvrwl rgnnlplppg srdlngrlei pniqldhggl yiceavgypa sttgqqvnvy
     1141 ltvekfeppa tnkphvcmyd eatcsngdci pksyvcngrl dctdgsdemr csphgcepne
     1201 frcnntqcvs klwrcdgdkd cadgsdeenc vpnrpgspcr vteytcasnd qcipksyhcd
     1261 mekdcidgsd eigcspvyim kppppmitld lgttlvivct aigvptpein wrlnwghipp
     1321 kctmtsingt gtltcpdiqt edqgayscea lntagfvfav pdaivmvnkp ddvcpkgmfn
     1381 seartveeci scfcfgvate chsanlftyq ipppfdrhki vavkqepdlr ilndisyqla
     1441 vkplgrdglh ldestpflnq laaekdvpyf alpenfhgsq lksyggylky rvryngtgvp
     1501 nsapavvlsg nnyvlihkgk qlipnydnde svrffegewl kkqgwsevpa tredimmtla
     1561 nvdnilikve ydnspdldvq lthivmdtad vrntglgsas fveecqcpsg ytglsceeca
     1621 pgylrrktgl wlgqcyrdep vdcppgyygd psrnipckac pcpltnpsnq fartchlgsd
     1681 gqptcncppg yigrrceqcd vgyqgnplip gdmcvarqqc dpegsissvv dpltgkcrck
     1741 qyatgitcnq ckantfknqf gctscfcmgi tnkcvssnwy rndvrvsftn sirdfslies
     1801 rtphpppivd girlntvtre iiyndfpnrg nndvyywqlp siflgdqvts yggnlkytvr
     1861 yvpapggqss knnaadveli sandinllyf srespepntp qtftvplleq ywqrndgtqa
     1921 drehllmala dvsairikat ytthtdeaal slvsldiaek yntgrarave veecscpigy
     1981 kglscedcdv gytrasegly lgiceqcncn ghsaqcnpdt gicencadht tgdfcevcep
     2041 gyegdatrgt phdcvykdst pcrcneagsr ssscigdrcd ckrnvegpqc nrcrpstfgl
     2101 ssynpdgcte cycsgvtnqc hesslyvqqi pvwvydnqhg ftltdstrqe iiddgfelnf
     2161 amneigyryp dsrgrrlfws lpaiftgnkv ksyggnltlt qhitaypgaq sykdqdiili
     2221 gngitlfwtn pmdiqpdipl tysvplrese wrrlttegpr vasridlmtv lsnleailvr
     2281 athsermtat yisdisldta vehqtgnrra vqvevcrcpt ghvgtscesc argyyrdpnd
     2341 rsasylgscn pcpcnnnees ceitrsgqvk chclpgytgq yclstgelmv sikpmkgevm
     2401 answilftcs yefpeplyis fkltpfggmp ltatgvtpgp imitekgaqr twyvyvqlep
     2461 cnvechiqdr dgkelikvvt svtpetnvtt ttpyppyipp prilvyikgp efqiveagtt
     2521 vryhcagksl dngslhikwe keggqlptgr tvddsqgllv irdvkvsdsg vyvcqvsdgv
     2581 higyknvtlt vggsnpvapr avvmptslev vegqpaefrc matghpqpqv ewirvhgpts
     2641 pevvthngiw slraasknda aeykciarnn vgvdeqtvil yvsdnpsmpp ttgilptitp
     2701 sqwygtegda vlltcsqtsp elaslvwlrq gnlpfsptat qrdgvltimn stqydsgvyi
     2761 clattyvgth vnntaeitvv prrsppsikv rpekqtvpqg svaevqcvts gepglqvkws
     2821 kyhherlssn vqqvgdtlri vniqipdrgv yvchvtgpsg shessaviev epreaprvei
     2881 yprevppvil ggstdlqcra vagipmpelh wshqngrpfp snikqlpggv lrlsnitand
     2941 ggayvcsavn pvgsntavvy ievqsvpvim isprsgilqv krgdrvklic rssgypqprv
     3001 awvkhqnrid frtpfylsse aatpleavye insaspddeg sytchannaa gmveervyir
     3061 vednevyspd eypgpdkpen pddqkitvpk dyfkipsggk vemrcyvrss dgnhiyldwk
     3121 rtdhrplpeg stvhngilni pvadksaage yvclgmdqar nvlfrakshl evlspprivl
     3181 npprqtvrag enpsidcstt gdepmtiqwa aigrnlpssv tqdrgvlqfh gitysdagky
     3241 vctatndagt aeavaevlvn ehpfdessvv haaqrdvvty agqpvrlrct vnvratihws
     3301 rdgqplpsta rieddylelp rarpedsgry icqiqtvhgv ssdyinlnvs lvnlhtecsr
     3361 anrtqcsesk cicnvqgcfl ldylcnvyir yyndigdnyy ntpqnrhylq qrratvtpav
     3421 sveasqnpvn igdtvnirca csgthnpryh wvrpnhpslp enaqeygnvl rltnvavsds
     3481 gvyrciadtp egvfdhdfnl vvhdgnndap aietkyapyg sslemdckpn lnpplkfhws
     3541 klggllprda qifenklkli nvkaedagty vctatnnlen ieiptvlvvt gvvpyfsqap
     3601 esfialpplp dsylkfniei sfkpesydgi ilyndesnrg ngdfillslv ggypvfsfdl
     3661 gsgptiirae ksvtlsewht iklqrnrkeg tmlvdgegpf kgvaagrkqg ldlkallfvg
     3721 gvpsdntvnk yaeitsgfvg cisrlvigek eidliddqtn svgitncetc aenpcnnagv
     3781 cqeaatkngy tclcragysg khcdyvgqsc ypgacgegtc vdketgfecy cphgktgsrc
     3841 ehsltiyepa fhddksfvah etpkalrrtr mnpsythnrl rarnaqvrka rsrphdhnkm
     3901 hhtkh
//
LOCUS       XP_034185288            3904 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185288
VERSION     XP_034185288.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329397.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3904
                     /product="terribly reduced optic lobes isoform X2"
                     /calculated_mol_wt=430405
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          800..834
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(805,813,824..825)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(817,820,824,830..831)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            827..831
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          838..872
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(843,851,862..863)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(855,858,862,868..869)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            865..869
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          874..908
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(879,887,898..899)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(891,894,898,904..905)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            901..905
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          922..956
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(927,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          960..991
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(965,973,984..985)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(977,980,984,990..991)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            987..991
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1007..1042
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1012,1021,1032..1033)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1025,1028,1032,1038..1039)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1035..1039
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1067..1143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1073..1077
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1086..1090
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1106..1110
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1157..1191
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1162,1170,1181..1182)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1174,1177,1181,1187..1188)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1184..1188
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1196..1230
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1201,1209,1220..1221)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1213,1216,1220,1226..1227)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1223..1227
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1239..1274
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1244,1253,1264..1265)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1257,1260,1264,1270..1271)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1267..1271
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1292..1367
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1295..1299
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1308..1312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1331..1335
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1345..1350
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1359..1362
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1469..1603
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1659..1712
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1660,1662,1675,1685,1687,1696)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1719..1765
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1720,1730,1737,1739,1748)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1836..1972
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2007..2055
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2007,2009,2016,2023,2026,2035)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2062..2108
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2062,2064,2074,2079,2081,2090)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2178..2314
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2510..2591
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2521..2525
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2536..2540
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2557..2561
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2605..2682
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2616..2620
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2629..2633
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2648..2652
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2662..2667
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2675..2678
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2700..2779
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2711..2715
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2724..2728
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2744..2748
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2758..2763
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2771..2774
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2792..2870
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2803..2807
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2816..2820
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2835..2840
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2850..2855
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2863..2866
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2887..2963
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2894..2898
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2908..2912
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2929..2933
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2943..2948
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2956..2959
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2978..3060
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2986..2990
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2999..3003
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3022..3030
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3040..3045
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3053..3056
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3084..3163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3100..3104
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3114..3119
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3135..3139
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3149..3154
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3157..3160
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3175..3258
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3192..3196
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3205..3209
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3224..3228
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3238..3243
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3251..3254
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3273..3348
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3284..3288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3295..3299
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3314..3318
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3328..3333
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3341..3344
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3428..3501
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3434..3438
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3447..3451
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3467..3471
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3481..3486
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3494..3497
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3519..3588
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3522..3526
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3535..3539
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3555..3558
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3568..3573
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3581..3584
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3594..3746
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3769..3802
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3904
                     /gene="trol"
                     /coded_by="XM_034329397.2:322..12036"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd eagcvnecpa
      841 gmfrcndglc ldskrrcdgr phcldgsdei ncqcpdgelp cdngvcinkn ffcdrnadch
      901 dgsderdcpv idettdgdhv acrqdefacp hggcipqsav cngrndcpye edeincprgc
      961 gkdqfqcsng dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag yimcvsdkdc
     1021 vpqssmcngi pecrdrsdee nceqlkepsr lnlktypseq vikenpakqg revvfqcrde
     1081 gplrarvrwl rgnnlplppg srdlngrlei pniqldhggl yiceavgypa sttgqqvnvy
     1141 ltvekfeppa tnkphvcmyd eatcsngdci pksyvcngrl dctdgsdemr csphgcepne
     1201 frcnntqcvs klwrcdgdkd cadgsdeenc vpnrpgspcr vteytcasnd qcipksyhcd
     1261 mekdcidgsd eigcspvyim kppppmitld lgttlvivct aigvptpein wrlnwghipp
     1321 kctmtsingt gtltcpdiqt edqgayscea lntagfvfav pdaivmvnkp ddvcpkgmfn
     1381 seartveeci scfcfgvate chsanlftyq ipppfdrhki vavkqepdlr ilndisyqla
     1441 vkplgrdglh ldestpflnq laaekdvpyf alpenfhgsq lksyggylky rvryngtgvp
     1501 nsapavvlsg nnyvlihkgk qlipnydnde svrffegewl kkqgwsevpa tredimmtla
     1561 nvdnilikve ydnspdldvq lthivmdtad vrntglgsas fveecqcpsg ytglsceeca
     1621 pgylrrktgl wlgqcyrdep vdcppgyygd psrnipckac pcpltnpsnq fartchlgsd
     1681 gqptcncppg yigrrceqcd vgyqgnplip gdmcvarqqc dpegsissvv dpltgkcrck
     1741 qyatgitcnq ckantfknqf gctscfcmgi tnkcvssnwy rndvrvsftn sirdfslies
     1801 rtphpppivd girlntvtre iiyndfpnrg nndvyywqlp siflgdqvts yggnlkytvr
     1861 yvpapggqss knnaadveli sandinllyf srespepntp qtftvplleq ywqrndgtqa
     1921 drehllmala dvsairikat ytthtdeaal slvsldiaek yntgrarave veecscpigy
     1981 kglscedcdv gytrasegly lgiceqcncn ghsaqcnpdt gicencadht tgdfcevcep
     2041 gyegdatrgt phdcvykdst pcrcneagsr ssscigdrcd ckrnvegpqc nrcrpstfgl
     2101 ssynpdgcte cycsgvtnqc hesslyvqqi pvwvydnqhg ftltdstrqe iiddgfelnf
     2161 amneigyryp dsrgrrlfws lpaiftgnkv ksyggnltlt qhitaypgaq sykdqdiili
     2221 gngitlfwtn pmdiqpdipl tysvplrese wrrlttegpr vasridlmtv lsnleailvr
     2281 athsermtat yisdisldta vehqtgnrra vqvevcrcpt ghvgtscesc argyyrdpnd
     2341 rsasylgscn pcpcnnnees ceitrsgqvk chclpgytgq yclstgelmv sikpmkgevm
     2401 answilftcs yefpeplyis fkltpfggmp ltatgvtpgp imitekgaqr twyvyvqlep
     2461 cnvechiqdr dgkelikvvt svtpetnvtt ttpyppyipp prilvyikgp efqiveagtt
     2521 vryhcagksl dngslhikwe keggqlptgr tvddsqgllv irdvkvsdsg vyvcqvsdgv
     2581 higyknvtlt vggsnpvapr avvmptslev vegqpaefrc matghpqpqv ewirvhgpts
     2641 pevvthngiw slraasknda aeykciarnn vgvdeqtvil yvsdnpsmpp ttgilptitp
     2701 sqwygtegda vlltcsqtsp elaslvwlrq gnlpfsptat qrdgvltimn stqydsgvyi
     2761 clattyvgth vnntaeitvv prrsppsikv rpekqtvpqg svaevqcvts gepglqvkws
     2821 kyhherlssn vqqvgdtlri vniqipdrgv yvchvtgpsg shessaviev epreaprvei
     2881 yprevppvil ggstdlqcra vagipmpelh wshqngrpfp snikqlpggv lrlsnitand
     2941 ggayvcsavn pvgsntavvy ievqsvpvim isprsgilqv krgdrvklic rssgypqprv
     3001 awvkhqnrid ltpfylssea atpleavyei nsaspddegs ytchannaag mveervyirv
     3061 ednevyspde ypgpdkpenp ddqkitvpkd yfkipsggkv emrcyvrssd gnhiyldwkr
     3121 tdhrplpegs tvhngilnip vadksaagey vclgmdqarn vlfrakshle vlspprivln
     3181 pprqtvrage npsidcsttg depmtiqwaa igrnlpssvt qdrgvlqfhg itysdagkyv
     3241 ctatndagta eavaevlvne hpfdessvvh aaqrdvvtya gqpvrlrctv nvratihwsr
     3301 dgqplpstar ieddylelpr arpedsgryi cqiqtvhgvs sdyinlnvsl vnlhtecsra
     3361 nrtqcseskc icnvqgcfll dylcnvyiry yndigdnyyn tpqnrhylqq rratvtpavs
     3421 veasqnpvni gdtvnircac sgthnpryhw vrpnhpslpe naqeygnvlr ltnvavsdsg
     3481 vyrciadtpe gvfdhdfnlv vhdgnndapa ietkyapygs slemdckpnl npplkfhwsk
     3541 lggllprdaq ifenklklin vkaedagtyv ctatnnleni eiptvlvvtg vvpyfsqape
     3601 sfialpplpd sylkfnieis fkpesydgii lyndesnrgn gdfillslvg gypvfsfdlg
     3661 sgptiiraek svtlsewhti klqrnrkegt mlvdgegpfk gvaagrkqgl dlkallfvgg
     3721 vpsdntvnky aeitsgfvgc isrlvigeke idliddqtns vgitncetca enpcnnagvc
     3781 qeaatkngyt clcragysgk hcdyvgqscy pgacgegtcv dketgfecyc phgktgsrce
     3841 hsltiyepaf hddksfvahe tpkalrrtrm npsythnrlr arnaqvrkar srphdhnkmh
     3901 htkh
//
LOCUS       XP_034185289            3900 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185289
VERSION     XP_034185289.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329398.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3900
                     /product="terribly reduced optic lobes isoform X3"
                     /calculated_mol_wt=430087
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          800..834
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(805,813,824..825)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(817,820,824,830..831)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            827..831
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          838..872
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(843,851,862..863)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(855,858,862,868..869)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            865..869
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          874..908
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(879,887,898..899)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(891,894,898,904..905)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            901..905
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          922..956
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(927,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          960..991
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(965,973,984..985)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(977,980,984,990..991)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            987..991
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1007..1042
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1012,1021,1032..1033)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1025,1028,1032,1038..1039)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1035..1039
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1057..1138
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1068..1072
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1081..1085
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1101..1105
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1152..1186
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1157,1165,1176..1177)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1169,1172,1176,1182..1183)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1179..1183
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1191..1225
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1196,1204,1215..1216)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1208,1211,1215,1221..1222)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1218..1222
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1234..1269
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1239,1248,1259..1260)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1252,1255,1259,1265..1266)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1262..1266
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1287..1362
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1290..1294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1303..1307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1326..1330
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1340..1345
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1354..1357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1464..1598
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1654..1707
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1655,1657,1670,1680,1682,1691)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1714..1760
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1715,1725,1732,1734,1743)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1831..1967
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2002..2050
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2002,2004,2011,2018,2021,2030)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2057..2103
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2057,2059,2069,2074,2076,2085)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2173..2309
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2505..2586
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2516..2520
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2531..2535
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2552..2556
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2600..2677
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2611..2615
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2624..2628
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2643..2647
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2657..2662
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2670..2673
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2774
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2706..2710
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2719..2723
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2739..2743
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2753..2758
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2766..2769
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2787..2865
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2798..2802
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2811..2815
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2830..2835
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2845..2850
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2858..2861
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2882..2958
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2889..2893
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2903..2907
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2924..2928
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2938..2943
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2951..2954
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2973..3056
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2981..2985
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2994..2998
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3018..3026
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3036..3041
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3049..3052
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3080..3159
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3096..3100
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3110..3115
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3131..3135
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3145..3150
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3153..3156
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3171..3254
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3188..3192
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3201..3205
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3220..3224
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3234..3239
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3250
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3269..3344
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3280..3284
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3291..3295
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3310..3314
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3324..3329
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3337..3340
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3424..3497
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3430..3434
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3443..3447
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3463..3467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3477..3482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3490..3493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3515..3584
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3518..3522
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3531..3535
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3551..3554
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3564..3569
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3577..3580
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3590..3742
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3765..3798
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3900
                     /gene="trol"
                     /coded_by="XM_034329398.2:322..12024"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd eagcvnecpa
      841 gmfrcndglc ldskrrcdgr phcldgsdei ncqcpdgelp cdngvcinkn ffcdrnadch
      901 dgsderdcpv idettdgdhv acrqdefacp hggcipqsav cngrndcpye edeincprgc
      961 gkdqfqcsng dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag yimcvsdkdc
     1021 vpqssmcngi pecrdrsdee nceqlkepsr lnlktypseq vikesrevvf qcrdegplra
     1081 rvrwlrgnnl plppgsrdln grleipniql dhgglyicea vgypasttgq qvnvyltvek
     1141 feppatnkph vcmydeatcs ngdcipksyv cngrldctdg sdemrcsphg cepnefrcnn
     1201 tqcvsklwrc dgdkdcadgs deencvpnrp gspcrvteyt casndqcipk syhcdmekdc
     1261 idgsdeigcs pvyimkpppp mitldlgttl vivctaigvp tpeinwrlnw ghippkctmt
     1321 singtgtltc pdiqtedqga yscealntag fvfavpdaiv mvnkpddvcp kgmfnseart
     1381 veeciscfcf gvatechsan lftyqipppf drhkivavkq epdlrilndi syqlavkplg
     1441 rdglhldest pflnqlaaek dvpyfalpen fhgsqlksyg gylkyrvryn gtgvpnsapa
     1501 vvlsgnnyvl ihkgkqlipn ydndesvrff egewlkkqgw sevpatredi mmtlanvdni
     1561 likveydnsp dldvqlthiv mdtadvrntg lgsasfveec qcpsgytgls ceecapgylr
     1621 rktglwlgqc yrdepvdcpp gyygdpsrni pckacpcplt npsnqfartc hlgsdgqptc
     1681 ncppgyigrr ceqcdvgyqg nplipgdmcv arqqcdpegs issvvdpltg kcrckqyatg
     1741 itcnqckant fknqfgctsc fcmgitnkcv ssnwyrndvr vsftnsirdf sliesrtphp
     1801 ppivdgirln tvtreiiynd fpnrgnndvy ywqlpsiflg dqvtsyggnl kytvryvpap
     1861 ggqssknnaa dvelisandi nllyfsresp epntpqtftv plleqywqrn dgtqadrehl
     1921 lmaladvsai rikatyttht deaalslvsl diaekyntgr araveveecs cpigykglsc
     1981 edcdvgytra seglylgice qcncnghsaq cnpdtgicen cadhttgdfc evcepgyegd
     2041 atrgtphdcv ykdstpcrcn eagsrsssci gdrcdckrnv egpqcnrcrp stfglssynp
     2101 dgctecycsg vtnqchessl yvqqipvwvy dnqhgftltd strqeiiddg felnfamnei
     2161 gyrypdsrgr rlfwslpaif tgnkvksygg nltltqhita ypgaqsykdq diiligngit
     2221 lfwtnpmdiq pdipltysvp lresewrrlt tegprvasri dlmtvlsnle ailvrathse
     2281 rmtatyisdi sldtavehqt gnrravqvev crcptghvgt scescargyy rdpndrsasy
     2341 lgscnpcpcn nneesceitr sgqvkchclp gytgqyclst gelmvsikpm kgevmanswi
     2401 lftcsyefpe plyisfkltp fggmpltatg vtpgpimite kgaqrtwyvy vqlepcnvec
     2461 hiqdrdgkel ikvvtsvtpe tnvttttpyp pyippprilv yikgpefqiv eagttvryhc
     2521 agksldngsl hikwekeggq lptgrtvdds qgllvirdvk vsdsgvyvcq vsdgvhigyk
     2581 nvtltvggsn pvapravvmp tslevvegqp aefrcmatgh pqpqvewirv hgptspevvt
     2641 hngiwslraa skndaaeykc iarnnvgvde qtvilyvsdn psmppttgil ptitpsqwyg
     2701 tegdavlltc sqtspelasl vwlrqgnlpf sptatqrdgv ltimnstqyd sgvyiclatt
     2761 yvgthvnnta eitvvprrsp psikvrpekq tvpqgsvaev qcvtsgepgl qvkwskyhhe
     2821 rlssnvqqvg dtlrivniqi pdrgvyvchv tgpsgshess avieveprea prveiyprev
     2881 ppvilggstd lqcravagip mpelhwshqn grpfpsnikq lpggvlrlsn itandggayv
     2941 csavnpvgsn tavvyievqs vpvimisprs gilqvkrgdr vklicrssgy pqprvawvkh
     3001 qnridfrtpf ylsseaatpl eavyeinsas pddegsytch annaagmvee rvyirvedne
     3061 vyspdeypgp dkpenpddqk itvpkdyfki psggkvemrc yvrssdgnhi yldwkrtdhr
     3121 plpegstvhn gilnipvadk saageyvclg mdqarnvlfr akshlevlsp privlnpprq
     3181 tvragenpsi dcsttgdepm tiqwaaigrn lpssvtqdrg vlqfhgitys dagkyvctat
     3241 ndagtaeava evlvnehpfd essvvhaaqr dvvtyagqpv rlrctvnvra tihwsrdgqp
     3301 lpstariedd ylelprarpe dsgryicqiq tvhgvssdyi nlnvslvnlh tecsranrtq
     3361 cseskcicnv qgcflldylc nvyiryyndi gdnyyntpqn rhylqqrrat vtpavsveas
     3421 qnpvnigdtv nircacsgth npryhwvrpn hpslpenaqe ygnvlrltnv avsdsgvyrc
     3481 iadtpegvfd hdfnlvvhdg nndapaietk yapygsslem dckpnlnppl kfhwsklggl
     3541 lprdaqifen klklinvkae dagtyvctat nnlenieipt vlvvtgvvpy fsqapesfia
     3601 lpplpdsylk fnieisfkpe sydgiilynd esnrgngdfi llslvggypv fsfdlgsgpt
     3661 iiraeksvtl sewhtiklqr nrkegtmlvd gegpfkgvaa grkqgldlka llfvggvpsd
     3721 ntvnkyaeit sgfvgcisrl vigekeidli ddqtnsvgit ncetcaenpc nnagvcqeaa
     3781 tkngytclcr agysgkhcdy vgqscypgac gegtcvdket gfecycphgk tgsrcehslt
     3841 iyepafhddk sfvahetpka lrrtrmnpsy thnrlrarna qvrkarsrph dhnkmhhtkh
//
LOCUS       XP_034185290            3893 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185290
VERSION     XP_034185290.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329399.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3893
                     /product="terribly reduced optic lobes isoform X4"
                     /calculated_mol_wt=429276
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          422..456
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(427,435,446..447)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(439,442,446,452..453)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            449..453
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          461..495
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(466,474,485..486)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(478,481,485,491..492)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            488..492
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          497..531
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(502,510,521..522)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(514,517,521,527..528)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            524..528
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          537..571
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(542,550,561..562)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(554,557,561,567..568)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            564..568
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          576..610
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(581,589,600..601)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(593,596,600,606..607)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            603..607
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          612..646
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(617,625,636..637)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(629,632,636,642..643)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            639..643
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          651..685
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(656,664,675..676)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(668,671,675,681..682)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            678..682
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          688..721
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(694,703,714..715)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(707,710,714,720..721)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            717..721
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          752..785
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(756,764,775..776)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(768,771,775,781..782)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            778..782
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          788..822
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(793,801,812..813)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(805,808,812,818..819)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            815..819
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          826..860
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(831,839,850..851)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(843,846,850,856..857)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            853..857
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          862..896
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(867,875,886..887)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(879,882,886,892..893)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            889..893
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          910..944
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(915,923,934..935)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(927,930,934,940..941)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            937..941
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          948..979
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(953,961,972..973)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(965,968,972,978..979)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            975..979
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          995..1030
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1000,1009,1020..1021)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1013,1016,1020,1026..1027)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1023..1027
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1055..1131
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1061..1065
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1074..1078
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1094..1098
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1145..1179
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1150,1158,1169..1170)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1162,1165,1169,1175..1176)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1172..1176
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1184..1218
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1189,1197,1208..1209)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1201,1204,1208,1214..1215)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1211..1215
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1227..1262
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1232,1241,1252..1253)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1245,1248,1252,1258..1259)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1255..1259
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1280..1355
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1283..1287
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1296..1300
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1319..1323
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1333..1338
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1347..1350
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1457..1591
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1647..1700
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1648,1650,1663,1673,1675,1684)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1707..1753
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1708,1718,1725,1727,1736)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1824..1960
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1995..2043
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1995,1997,2004,2011,2014,2023)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2050..2096
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2050,2052,2062,2067,2069,2078)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2166..2302
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2498..2579
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2509..2513
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2524..2528
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2545..2549
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2593..2670
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2604..2608
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2617..2621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2636..2640
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2650..2655
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2663..2666
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2688..2767
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2699..2703
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2712..2716
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2732..2736
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2746..2751
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2759..2762
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2780..2858
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2791..2795
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2804..2808
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2823..2828
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2838..2843
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2851..2854
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2875..2951
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2882..2886
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2896..2900
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2917..2921
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2931..2936
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2944..2947
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2966..3049
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2974..2978
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2987..2991
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3011..3019
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3029..3034
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3042..3045
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3073..3152
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3089..3093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3103..3108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3124..3128
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3138..3143
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3146..3149
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3164..3247
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3181..3185
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3194..3198
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3213..3217
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3227..3232
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3240..3243
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3262..3337
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3273..3277
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3285..3289
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3303..3307
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3317..3322
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3331..3334
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3417..3490
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3423..3427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3436..3440
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3456..3460
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3470..3475
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3483..3486
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3508..3577
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3511..3515
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3524..3528
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3544..3547
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3557..3562
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3570..3573
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3583..3735
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3758..3791
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3893
                     /gene="trol"
                     /coded_by="XM_034329399.2:322..12003"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdrdhktvt epyqepeesv
      361 esttnqdeee gitsrpsvnk craddtvrcs dgsrsicsvq rcdgvpdcdd ggdevgcphp
      421 gcsagefacd vsrcileshr cnfikecddg sdehdcnypa ctstqfkcrn gqcidssahc
      481 ngvedcrdrt delncpcred qfectpgfcv plnrrcdrfm dctngrdeen csnmtrcrpd
      541 eftcsdggcv sqnarcdgrs dcpdrsdehn cnvttcsgdq frcldgtcis idkrcnsnid
      601 crngedenqc gcgeakfrct dgqcigyelq cngveecsdg sderdcdlap cpsmdftcsd
      661 gsciskssvc dgfddcprae delhcerect ptqfkcvtgn kciegvyrcd ghpdcpdhsd
      721 edcanetith aiqptsqpwp gratdslrec dpsremrcdd gkcvllrrkc dnifdcidgs
      781 dergcgvctp aewkcasgec ipetercdnv vqcadgtdea gcvnecpagm frcndglcld
      841 skrrcdgrph cldgsdeinc qcpdgelpcd ngvcinknff cdrnadchdg sderdcpvid
      901 ettdgdhvac rqdefacphg gcipqsavcn grndcpyeed eincprgcgk dqfqcsngdc
      961 iradqkcngy idcvdgsdep eecerpgfhp mpgrcpagyi mcvsdkdcvp qssmcngipe
     1021 crdrsdeenc eqlkepsrln lktypseqvi kenpakqgre vvfqcrdegp lrarvrwlrg
     1081 nnlplppgsr dlngrleipn iqldhgglyi ceavgypast tgqqvnvylt vekfeppatn
     1141 kphvcmydea tcsngdcipk syvcngrldc tdgsdemrcs phgcepnefr cnntqcvskl
     1201 wrcdgdkdca dgsdeencvp nrpgspcrvt eytcasndqc ipksyhcdme kdcidgsdei
     1261 gcspvyimkp pppmitldlg ttlvivctai gvptpeinwr lnwghippkc tmtsingtgt
     1321 ltcpdiqted qgayscealn tagfvfavpd aivmvnkpdd vcpkgmfnse artveecisc
     1381 fcfgvatech sanlftyqip ppfdrhkiva vkqepdlril ndisyqlavk plgrdglhld
     1441 estpflnqla aekdvpyfal penfhgsqlk syggylkyrv ryngtgvpns apavvlsgnn
     1501 yvlihkgkql ipnydndesv rffegewlkk qgwsevpatr edimmtlanv dnilikveyd
     1561 nspdldvqlt hivmdtadvr ntglgsasfv eecqcpsgyt glsceecapg ylrrktglwl
     1621 gqcyrdepvd cppgyygdps rnipckacpc pltnpsnqfa rtchlgsdgq ptcncppgyi
     1681 grrceqcdvg yqgnplipgd mcvarqqcdp egsissvvdp ltgkcrckqy atgitcnqck
     1741 antfknqfgc tscfcmgitn kcvssnwyrn dvrvsftnsi rdfsliesrt phpppivdgi
     1801 rlntvtreii yndfpnrgnn dvyywqlpsi flgdqvtsyg gnlkytvryv papggqsskn
     1861 naadvelisa ndinllyfsr espepntpqt ftvplleqyw qrndgtqadr ehllmaladv
     1921 sairikatyt thtdeaalsl vsldiaekyn tgraraveve ecscpigykg lscedcdvgy
     1981 traseglylg iceqcncngh saqcnpdtgi cencadhttg dfcevcepgy egdatrgtph
     2041 dcvykdstpc rcneagsrss scigdrcdck rnvegpqcnr crpstfglss ynpdgctecy
     2101 csgvtnqche sslyvqqipv wvydnqhgft ltdstrqeii ddgfelnfam neigyrypds
     2161 rgrrlfwslp aiftgnkvks yggnltltqh itaypgaqsy kdqdiilign gitlfwtnpm
     2221 diqpdiplty svplresewr rlttegprva sridlmtvls nleailvrat hsermtatyi
     2281 sdisldtave hqtgnrravq vevcrcptgh vgtscescar gyyrdpndrs asylgscnpc
     2341 pcnnneesce itrsgqvkch clpgytgqyc lstgelmvsi kpmkgevman swilftcsye
     2401 fpeplyisfk ltpfggmplt atgvtpgpim itekgaqrtw yvyvqlepcn vechiqdrdg
     2461 kelikvvtsv tpetnvtttt pyppyipppr ilvyikgpef qiveagttvr yhcagksldn
     2521 gslhikweke ggqlptgrtv ddsqgllvir dvkvsdsgvy vcqvsdgvhi gyknvtltvg
     2581 gsnpvaprav vmptslevve gqpaefrcma tghpqpqvew irvhgptspe vvthngiwsl
     2641 raaskndaae ykciarnnvg vdeqtvilyv sdnpsmpptt gilptitpsq wygtegdavl
     2701 ltcsqtspel aslvwlrqgn lpfsptatqr dgvltimnst qydsgvyicl attyvgthvn
     2761 ntaeitvvpr rsppsikvrp ekqtvpqgsv aevqcvtsge pglqvkwsky hherlssnvq
     2821 qvgdtlrivn iqipdrgvyv chvtgpsgsh essavievep reaprveiyp revppvilgg
     2881 stdlqcrava gipmpelhws hqngrpfpsn ikqlpggvlr lsnitandgg ayvcsavnpv
     2941 gsntavvyie vqsvpvimis prsgilqvkr gdrvklicrs sgypqprvaw vkhqnridfr
     3001 tpfylsseaa tpleavyein saspddegsy tchannaagm veervyirve dnevyspdey
     3061 pgpdkpenpd dqkitvpkdy fkipsggkve mrcyvrssdg nhiyldwkrt dhrplpegst
     3121 vhngilnipv adksaageyv clgmdqarnv lfrakshlev lspprivlnp prqtvragen
     3181 psidcsttgd epmtiqwaai grnlpssvtq drgvlqfhgi tysdagkyvc tatndagtae
     3241 avaevlvneh pfdessvvha aqrdvvtyag qpvrlrctvn vratihwsrd gqplpstari
     3301 eddylelpra rpedsgryic qiqtvhgvss dyinlnvslv nlhtecsran rtqcseskci
     3361 cnvqgcflld ylcnvyiryy ndigdnyynt pqnrhylqqr ratvtpavsv easqnpvnig
     3421 dtvnircacs gthnpryhwv rpnhpslpen aqeygnvlrl tnvavsdsgv yrciadtpeg
     3481 vfdhdfnlvv hdgnndapai etkyapygss lemdckpnln pplkfhwskl ggllprdaqi
     3541 fenklklinv kaedagtyvc tatnnlenie iptvlvvtgv vpyfsqapes fialpplpds
     3601 ylkfnieisf kpesydgiil yndesnrgng dfillslvgg ypvfsfdlgs gptiiraeks
     3661 vtlsewhtik lqrnrkegtm lvdgegpfkg vaagrkqgld lkallfvggv psdntvnkya
     3721 eitsgfvgci srlvigekei dliddqtnsv gitncetcae npcnnagvcq eaatkngytc
     3781 lcragysgkh cdyvgqscyp gacgegtcvd ketgfecycp hgktgsrceh sltiyepafh
     3841 ddksfvahet pkalrrtrmn psythnrlra rnaqvrkars rphdhnkmhh tkh
//
LOCUS       XP_034185291            3869 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034185291
VERSION     XP_034185291.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329400.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3869
                     /product="terribly reduced optic lobes isoform X5"
                     /calculated_mol_wt=426736
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          627..661
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(632,640,651..652)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(644,647,651,657..658)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            654..658
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          664..697
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(670,679,690..691)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(683,686,690,696..697)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            693..697
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          728..761
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(732,740,751..752)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(744,747,751,757..758)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            754..758
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..798
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(769,777,788..789)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(781,784,788,794..795)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            791..795
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          802..836
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(807,815,826..827)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(819,822,826,832..833)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            829..833
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          838..872
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(843,851,862..863)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(855,858,862,868..869)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            865..869
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          886..920
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(891,899,910..911)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(903,906,910,916..917)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            913..917
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          924..955
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(929,937,948..949)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(941,944,948,954..955)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            951..955
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          971..1006
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(976,985,996..997)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(989,992,996,1002..1003)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            999..1003
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1031..1107
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1037..1041
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1050..1054
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1070..1074
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1121..1155
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1126,1134,1145..1146)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1138,1141,1145,1151..1152)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1148..1152
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1160..1194
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1165,1173,1184..1185)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1177,1180,1184,1190..1191)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1187..1191
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1203..1238
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1208,1217,1228..1229)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1221,1224,1228,1234..1235)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1231..1235
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1256..1331
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1259..1263
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1272..1276
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1295..1299
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1309..1314
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1323..1326
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1433..1567
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1623..1676
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1624,1626,1639,1649,1651,1660)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1683..1729
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1684,1694,1701,1703,1712)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1800..1936
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1971..2019
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1971,1973,1980,1987,1990,1999)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2026..2072
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2026,2028,2038,2043,2045,2054)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2142..2278
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2474..2555
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2485..2489
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2500..2504
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2521..2525
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2569..2646
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2580..2584
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2593..2597
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2612..2616
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2626..2631
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2639..2642
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2664..2743
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2675..2679
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2688..2692
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2708..2712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2722..2727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2735..2738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2756..2834
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2767..2771
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2780..2784
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2799..2804
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2814..2819
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2827..2830
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2851..2927
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2858..2862
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2872..2876
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2897
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2907..2912
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2920..2923
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2942..3025
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2950..2954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2963..2967
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2987..2995
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3005..3010
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3018..3021
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3049..3128
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3065..3069
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3079..3084
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3100..3104
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3114..3119
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3122..3125
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3140..3223
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3157..3161
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3170..3174
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3189..3193
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3203..3208
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3216..3219
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3238..3313
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3249..3253
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3264
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3279..3283
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3293..3298
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3306..3309
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3393..3466
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3399..3403
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3412..3416
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3432..3436
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3446..3451
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3459..3462
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3484..3553
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3487..3491
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3500..3504
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3520..3523
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3533..3538
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3546..3549
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3559..3711
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3734..3767
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3869
                     /gene="trol"
                     /coded_by="XM_034329400.2:322..11931"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcdlapcpsm dftcsdgsci skssvcdgfd dcpraedelh
      661 cerectptqf kcvtgnkcie gvyrcdghpd cpdhsdedca netithaiqp tsqpwpgrat
      721 dslrecdpsr emrcddgkcv llrrkcdnif dcidgsderg cgvctpaewk casgecipet
      781 ercdnvvqca dgtdeagcvn ecpagmfrcn dglcldskrr cdgrphcldg sdeincqcpd
      841 gelpcdngvc inknffcdrn adchdgsder dcpvidettd gdhvacrqde facphggcip
      901 qsavcngrnd cpyeedeinc prgcgkdqfq csngdcirad qkcngyidcv dgsdepeece
      961 rpgfhpmpgr cpagyimcvs dkdcvpqssm cngipecrdr sdeenceqlk epsrlnlkty
     1021 pseqvikenp akqgrevvfq crdegplrar vrwlrgnnlp lppgsrdlng rleipniqld
     1081 hgglyiceav gypasttgqq vnvyltvekf eppatnkphv cmydeatcsn gdcipksyvc
     1141 ngrldctdgs demrcsphgc epnefrcnnt qcvsklwrcd gdkdcadgsd eencvpnrpg
     1201 spcrvteytc asndqcipks yhcdmekdci dgsdeigcsp vyimkppppm itldlgttlv
     1261 ivctaigvpt peinwrlnwg hippkctmts ingtgtltcp diqtedqgay scealntagf
     1321 vfavpdaivm vnkpddvcpk gmfnseartv eeciscfcfg vatechsanl ftyqipppfd
     1381 rhkivavkqe pdlrilndis yqlavkplgr dglhldestp flnqlaaekd vpyfalpenf
     1441 hgsqlksygg ylkyrvryng tgvpnsapav vlsgnnyvli hkgkqlipny dndesvrffe
     1501 gewlkkqgws evpatredim mtlanvdnil ikveydnspd ldvqlthivm dtadvrntgl
     1561 gsasfveecq cpsgytglsc eecapgylrr ktglwlgqcy rdepvdcppg yygdpsrnip
     1621 ckacpcpltn psnqfartch lgsdgqptcn cppgyigrrc eqcdvgyqgn plipgdmcva
     1681 rqqcdpegsi ssvvdpltgk crckqyatgi tcnqckantf knqfgctscf cmgitnkcvs
     1741 snwyrndvrv sftnsirdfs liesrtphpp pivdgirlnt vtreiiyndf pnrgnndvyy
     1801 wqlpsiflgd qvtsyggnlk ytvryvpapg gqssknnaad velisandin llyfsrespe
     1861 pntpqtftvp lleqywqrnd gtqadrehll maladvsair ikatytthtd eaalslvsld
     1921 iaekyntgra raveveecsc pigykglsce dcdvgytras eglylgiceq cncnghsaqc
     1981 npdtgicenc adhttgdfce vcepgyegda trgtphdcvy kdstpcrcne agsrssscig
     2041 drcdckrnve gpqcnrcrps tfglssynpd gctecycsgv tnqchessly vqqipvwvyd
     2101 nqhgftltds trqeiiddgf elnfamneig yrypdsrgrr lfwslpaift gnkvksyggn
     2161 ltltqhitay pgaqsykdqd iiligngitl fwtnpmdiqp dipltysvpl resewrrltt
     2221 egprvasrid lmtvlsnlea ilvrathser mtatyisdis ldtavehqtg nrravqvevc
     2281 rcptghvgts cescargyyr dpndrsasyl gscnpcpcnn neesceitrs gqvkchclpg
     2341 ytgqyclstg elmvsikpmk gevmanswil ftcsyefpep lyisfkltpf ggmpltatgv
     2401 tpgpimitek gaqrtwyvyv qlepcnvech iqdrdgkeli kvvtsvtpet nvttttpypp
     2461 yippprilvy ikgpefqive agttvryhca gksldngslh ikwekeggql ptgrtvddsq
     2521 gllvirdvkv sdsgvyvcqv sdgvhigykn vtltvggsnp vapravvmpt slevvegqpa
     2581 efrcmatghp qpqvewirvh gptspevvth ngiwslraas kndaaeykci arnnvgvdeq
     2641 tvilyvsdnp smppttgilp titpsqwygt egdavlltcs qtspelaslv wlrqgnlpfs
     2701 ptatqrdgvl timnstqyds gvyiclatty vgthvnntae itvvprrspp sikvrpekqt
     2761 vpqgsvaevq cvtsgepglq vkwskyhher lssnvqqvgd tlrivniqip drgvyvchvt
     2821 gpsgshessa vievepreap rveiyprevp pvilggstdl qcravagipm pelhwshqng
     2881 rpfpsnikql pggvlrlsni tandggayvc savnpvgsnt avvyievqsv pvimisprsg
     2941 ilqvkrgdrv klicrssgyp qprvawvkhq nridfrtpfy lsseaatple avyeinsasp
     3001 ddegsytcha nnaagmveer vyirvednev yspdeypgpd kpenpddqki tvpkdyfkip
     3061 sggkvemrcy vrssdgnhiy ldwkrtdhrp lpegstvhng ilnipvadks aageyvclgm
     3121 dqarnvlfra kshlevlspp rivlnpprqt vragenpsid csttgdepmt iqwaaigrnl
     3181 pssvtqdrgv lqfhgitysd agkyvctatn dagtaeavae vlvnehpfde ssvvhaaqrd
     3241 vvtyagqpvr lrctvnvrat ihwsrdgqpl pstarieddy lelprarped sgryicqiqt
     3301 vhgvssdyin lnvslvnlht ecsranrtqc seskcicnvq gcflldylcn vyiryyndig
     3361 dnyyntpqnr hylqqrratv tpavsveasq npvnigdtvn ircacsgthn pryhwvrpnh
     3421 pslpenaqey gnvlrltnva vsdsgvyrci adtpegvfdh dfnlvvhdgn ndapaietky
     3481 apygsslemd ckpnlnpplk fhwsklggll prdaqifenk lklinvkaed agtyvctatn
     3541 nlenieiptv lvvtgvvpyf sqapesfial pplpdsylkf nieisfkpes ydgiilynde
     3601 snrgngdfil lslvggypvf sfdlgsgpti iraeksvtls ewhtiklqrn rkegtmlvdg
     3661 egpfkgvaag rkqgldlkal lfvggvpsdn tvnkyaeits gfvgcisrlv igekeidlid
     3721 dqtnsvgitn cetcaenpcn nagvcqeaat kngytclcra gysgkhcdyv gqscypgacg
     3781 egtcvdketg fecycphgkt gsrcehslti yepafhddks fvahetpkal rrtrmnpsyt
     3841 hnrlrarnaq vrkarsrphd hnkmhhtkh
//
LOCUS       XP_034185292            3867 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034185292
VERSION     XP_034185292.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329401.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3867
                     /product="terribly reduced optic lobes isoform X6"
                     /calculated_mol_wt=426414
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          800..834
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(805,813,824..825)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(817,820,824,830..831)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            827..831
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          836..870
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(841,849,860..861)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(853,856,860,866..867)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            863..867
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          884..918
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(889,897,908..909)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(901,904,908,914..915)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            911..915
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          922..953
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(927,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          969..1004
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(974,983,994..995)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(987,990,994,1000..1001)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            997..1001
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1029..1105
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1035..1039
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1048..1052
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1068..1072
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1119..1153
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1124,1132,1143..1144)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1136,1139,1143,1149..1150)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1146..1150
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1158..1192
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1163,1171,1182..1183)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1175,1178,1182,1188..1189)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1185..1189
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1201..1236
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1206,1215,1226..1227)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1219,1222,1226,1232..1233)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1229..1233
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1254..1329
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1257..1261
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1270..1274
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1293..1297
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1307..1312
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1321..1324
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1431..1565
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1621..1674
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1622,1624,1637,1647,1649,1658)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1681..1727
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1682,1692,1699,1701,1710)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1798..1934
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1969..2017
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1969,1971,1978,1985,1988,1997)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2024..2070
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2024,2026,2036,2041,2043,2052)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2140..2276
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2472..2553
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2483..2487
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2498..2502
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2519..2523
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2567..2644
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2578..2582
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2591..2595
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2610..2614
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2624..2629
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2637..2640
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2662..2741
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2673..2677
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2686..2690
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2706..2710
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2720..2725
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2733..2736
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2754..2832
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2765..2769
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2778..2782
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2797..2802
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2812..2817
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2825..2828
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2849..2925
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2856..2860
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2870..2874
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2891..2895
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2905..2910
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2918..2921
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2940..3023
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2948..2952
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2961..2965
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2985..2993
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3008
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3016..3019
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3047..3126
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3063..3067
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3077..3082
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3098..3102
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3112..3117
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3120..3123
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3138..3221
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3155..3159
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3168..3172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3187..3191
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3201..3206
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3214..3217
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3236..3311
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3247..3251
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3259..3263
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3277..3281
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3291..3296
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3305..3308
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3391..3464
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3397..3401
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3410..3414
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3430..3434
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3444..3449
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3457..3460
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3482..3551
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3485..3489
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3498..3502
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3518..3521
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3531..3536
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3544..3547
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3557..3709
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3732..3765
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3867
                     /gene="trol"
                     /coded_by="XM_034329401.2:322..11925"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd eagcecpdge
      841 lpcdngvcin knffcdrnad chdgsderdc pvidettdgd hvacrqdefa cphggcipqs
      901 avcngrndcp yeedeincpr gcgkdqfqcs ngdciradqk cngyidcvdg sdepeecerp
      961 gfhpmpgrcp agyimcvsdk dcvpqssmcn gipecrdrsd eenceqlkep srlnlktyps
     1021 eqvikenpak qgrevvfqcr degplrarvr wlrgnnlplp pgsrdlngrl eipniqldhg
     1081 glyiceavgy pasttgqqvn vyltvekfep patnkphvcm ydeatcsngd cipksyvcng
     1141 rldctdgsde mrcsphgcep nefrcnntqc vsklwrcdgd kdcadgsdee ncvpnrpgsp
     1201 crvteytcas ndqcipksyh cdmekdcidg sdeigcspvy imkppppmit ldlgttlviv
     1261 ctaigvptpe inwrlnwghi ppkctmtsin gtgtltcpdi qtedqgaysc ealntagfvf
     1321 avpdaivmvn kpddvcpkgm fnseartvee ciscfcfgva techsanlft yqipppfdrh
     1381 kivavkqepd lrilndisyq lavkplgrdg lhldestpfl nqlaaekdvp yfalpenfhg
     1441 sqlksyggyl kyrvryngtg vpnsapavvl sgnnyvlihk gkqlipnydn desvrffege
     1501 wlkkqgwsev patredimmt lanvdnilik veydnspdld vqlthivmdt advrntglgs
     1561 asfveecqcp sgytglscee capgylrrkt glwlgqcyrd epvdcppgyy gdpsrnipck
     1621 acpcpltnps nqfartchlg sdgqptcncp pgyigrrceq cdvgyqgnpl ipgdmcvarq
     1681 qcdpegsiss vvdpltgkcr ckqyatgitc nqckantfkn qfgctscfcm gitnkcvssn
     1741 wyrndvrvsf tnsirdfsli esrtphpppi vdgirlntvt reiiyndfpn rgnndvyywq
     1801 lpsiflgdqv tsyggnlkyt vryvpapggq ssknnaadve lisandinll yfsrespepn
     1861 tpqtftvpll eqywqrndgt qadrehllma ladvsairik atytthtdea alslvsldia
     1921 ekyntgrara veveecscpi gykglscedc dvgytraseg lylgiceqcn cnghsaqcnp
     1981 dtgicencad httgdfcevc epgyegdatr gtphdcvykd stpcrcneag srssscigdr
     2041 cdckrnvegp qcnrcrpstf glssynpdgc tecycsgvtn qchesslyvq qipvwvydnq
     2101 hgftltdstr qeiiddgfel nfamneigyr ypdsrgrrlf wslpaiftgn kvksyggnlt
     2161 ltqhitaypg aqsykdqdii ligngitlfw tnpmdiqpdi pltysvplre sewrrltteg
     2221 prvasridlm tvlsnleail vrathsermt atyisdisld tavehqtgnr ravqvevcrc
     2281 ptghvgtsce scargyyrdp ndrsasylgs cnpcpcnnne esceitrsgq vkchclpgyt
     2341 gqyclstgel mvsikpmkge vmanswilft csyefpeply isfkltpfgg mpltatgvtp
     2401 gpimitekga qrtwyvyvql epcnvechiq drdgkelikv vtsvtpetnv ttttpyppyi
     2461 ppprilvyik gpefqiveag ttvryhcagk sldngslhik wekeggqlpt grtvddsqgl
     2521 lvirdvkvsd sgvyvcqvsd gvhigyknvt ltvggsnpva pravvmptsl evvegqpaef
     2581 rcmatghpqp qvewirvhgp tspevvthng iwslraaskn daaeykciar nnvgvdeqtv
     2641 ilyvsdnpsm ppttgilpti tpsqwygteg davlltcsqt spelaslvwl rqgnlpfspt
     2701 atqrdgvlti mnstqydsgv yiclattyvg thvnntaeit vvprrsppsi kvrpekqtvp
     2761 qgsvaevqcv tsgepglqvk wskyhherls snvqqvgdtl rivniqipdr gvyvchvtgp
     2821 sgshessavi evepreaprv eiyprevppv ilggstdlqc ravagipmpe lhwshqngrp
     2881 fpsnikqlpg gvlrlsnita ndggayvcsa vnpvgsntav vyievqsvpv imisprsgil
     2941 qvkrgdrvkl icrssgypqp rvawvkhqnr idfrtpfyls seaatpleav yeinsaspdd
     3001 egsytchann aagmveervy irvednevys pdeypgpdkp enpddqkitv pkdyfkipsg
     3061 gkvemrcyvr ssdgnhiyld wkrtdhrplp egstvhngil nipvadksaa geyvclgmdq
     3121 arnvlfraks hlevlsppri vlnpprqtvr agenpsidcs ttgdepmtiq waaigrnlps
     3181 svtqdrgvlq fhgitysdag kyvctatnda gtaeavaevl vnehpfdess vvhaaqrdvv
     3241 tyagqpvrlr ctvnvratih wsrdgqplps tarieddyle lprarpedsg ryicqiqtvh
     3301 gvssdyinln vslvnlhtec sranrtqcse skcicnvqgc flldylcnvy iryyndigdn
     3361 yyntpqnrhy lqqrratvtp avsveasqnp vnigdtvnir cacsgthnpr yhwvrpnhps
     3421 lpenaqeygn vlrltnvavs dsgvyrciad tpegvfdhdf nlvvhdgnnd apaietkyap
     3481 ygsslemdck pnlnpplkfh wsklggllpr daqifenklk linvkaedag tyvctatnnl
     3541 enieiptvlv vtgvvpyfsq apesfialpp lpdsylkfni eisfkpesyd giilyndesn
     3601 rgngdfills lvggypvfsf dlgsgptiir aeksvtlsew htiklqrnrk egtmlvdgeg
     3661 pfkgvaagrk qgldlkallf vggvpsdntv nkyaeitsgf vgcisrlvig ekeidliddq
     3721 tnsvgitnce tcaenpcnna gvcqeaatkn gytclcragy sgkhcdyvgq scypgacgeg
     3781 tcvdketgfe cycphgktgs rcehsltiye pafhddksfv ahetpkalrr trmnpsythn
     3841 rlrarnaqvr karsrphdhn kmhhtkh
//
LOCUS       XP_034185293            3866 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034185293
VERSION     XP_034185293.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329402.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3866
                     /product="terribly reduced optic lobes isoform X7"
                     /calculated_mol_wt=426020
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          510..544
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(515,523,534..535)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(527,530,534,540..541)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            537..541
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          585..619
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(590,598,609..610)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(602,605,609,615..616)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            612..616
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          661..694
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(667,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          725..758
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(729,737,748..749)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(741,744,748,754..755)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            751..755
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          761..795
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(766,774,785..786)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(778,781,785,791..792)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            788..792
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          799..833
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(804,812,823..824)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(816,819,823,829..830)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            826..830
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          835..869
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(840,848,859..860)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(852,855,859,865..866)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            862..866
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          883..917
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(888,896,907..908)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(900,903,907,913..914)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            910..914
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          921..952
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(926,934,945..946)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(938,941,945,951..952)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            948..952
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          968..1003
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(973,982,993..994)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(986,989,993,999..1000)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            996..1000
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1028..1104
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1034..1038
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1047..1051
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1067..1071
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1118..1152
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1123,1131,1142..1143)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1135,1138,1142,1148..1149)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1145..1149
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1157..1191
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1162,1170,1181..1182)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1174,1177,1181,1187..1188)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1184..1188
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1200..1235
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1205,1214,1225..1226)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1218,1221,1225,1231..1232)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1228..1232
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1253..1328
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1256..1260
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1269..1273
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1292..1296
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1306..1311
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1320..1323
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1430..1564
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1620..1673
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1621,1623,1636,1646,1648,1657)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1680..1726
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1681,1691,1698,1700,1709)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1797..1933
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1968..2016
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1968,1970,1977,1984,1987,1996)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2023..2069
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2023,2025,2035,2040,2042,2051)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2139..2275
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2471..2552
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2482..2486
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2497..2501
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2518..2522
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2643
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2577..2581
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2590..2594
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2609..2613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2623..2628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2636..2639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2661..2740
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2672..2676
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2685..2689
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2705..2709
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2719..2724
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2732..2735
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2753..2831
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2764..2768
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2781
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2796..2801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2811..2816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2824..2827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2848..2924
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2855..2859
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2869..2873
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2890..2894
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2904..2909
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2917..2920
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2939..3022
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2947..2951
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2960..2964
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2984..2992
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3002..3007
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3015..3018
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3046..3125
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3062..3066
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3076..3081
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3097..3101
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3111..3116
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3119..3122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3137..3220
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3154..3158
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3167..3171
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3186..3190
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3200..3205
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3213..3216
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3235..3310
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3246..3250
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3258..3262
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3276..3280
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3290..3295
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3307
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3390..3463
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3396..3400
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3409..3413
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3429..3433
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3443..3448
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3456..3459
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3481..3550
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3484..3488
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3497..3501
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3517..3520
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3530..3535
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3543..3546
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3556..3708
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3731..3764
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3866
                     /gene="trol"
                     /coded_by="XM_034329402.2:322..11922"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncprc rpdeftcsdg gcvsqnarcd grsdcpdrsd
      541 ehncnvttcs gdqfrcldgt cisidkrcns nidcrngede nqcgcgeakf rctdgqcigy
      601 elqcngveec sdgsderdcd lapcpsmdft csdgscisks svcdgfddcp raedelhcer
      661 ectptqfkcv tgnkciegvy rcdghpdcpd hsdedcanet ithaiqptsq pwpgratdsl
      721 recdpsremr cddgkcvllr rkcdnifdci dgsdergcgv ctpaewkcas gecipeterc
      781 dnvvqcadgt deagcvnecp agmfrcndgl cldskrrcdg rphcldgsde incqcpdgel
      841 pcdngvcink nffcdrnadc hdgsderdcp videttdgdh vacrqdefac phggcipqsa
      901 vcngrndcpy eedeincprg cgkdqfqcsn gdciradqkc ngyidcvdgs depeecerpg
      961 fhpmpgrcpa gyimcvsdkd cvpqssmcng ipecrdrsde enceqlkeps rlnlktypse
     1021 qvikenpakq grevvfqcrd egplrarvrw lrgnnlplpp gsrdlngrle ipniqldhgg
     1081 lyiceavgyp asttgqqvnv yltvekfepp atnkphvcmy deatcsngdc ipksyvcngr
     1141 ldctdgsdem rcsphgcepn efrcnntqcv sklwrcdgdk dcadgsdeen cvpnrpgspc
     1201 rvteytcasn dqcipksyhc dmekdcidgs deigcspvyi mkppppmitl dlgttlvivc
     1261 taigvptpei nwrlnwghip pkctmtsing tgtltcpdiq tedqgaysce alntagfvfa
     1321 vpdaivmvnk pddvcpkgmf nseartveec iscfcfgvat echsanlfty qipppfdrhk
     1381 ivavkqepdl rilndisyql avkplgrdgl hldestpfln qlaaekdvpy falpenfhgs
     1441 qlksyggylk yrvryngtgv pnsapavvls gnnyvlihkg kqlipnydnd esvrffegew
     1501 lkkqgwsevp atredimmtl anvdnilikv eydnspdldv qlthivmdta dvrntglgsa
     1561 sfveecqcps gytglsceec apgylrrktg lwlgqcyrde pvdcppgyyg dpsrnipcka
     1621 cpcpltnpsn qfartchlgs dgqptcncpp gyigrrceqc dvgyqgnpli pgdmcvarqq
     1681 cdpegsissv vdpltgkcrc kqyatgitcn qckantfknq fgctscfcmg itnkcvssnw
     1741 yrndvrvsft nsirdfslie srtphpppiv dgirlntvtr eiiyndfpnr gnndvyywql
     1801 psiflgdqvt syggnlkytv ryvpapggqs sknnaadvel isandinlly fsrespepnt
     1861 pqtftvplle qywqrndgtq adrehllmal advsairika tytthtdeaa lslvsldiae
     1921 kyntgrarav eveecscpig ykglscedcd vgytrasegl ylgiceqcnc nghsaqcnpd
     1981 tgicencadh ttgdfcevce pgyegdatrg tphdcvykds tpcrcneags rssscigdrc
     2041 dckrnvegpq cnrcrpstfg lssynpdgct ecycsgvtnq chesslyvqq ipvwvydnqh
     2101 gftltdstrq eiiddgfeln famneigyry pdsrgrrlfw slpaiftgnk vksyggnltl
     2161 tqhitaypga qsykdqdiil igngitlfwt npmdiqpdip ltysvplres ewrrlttegp
     2221 rvasridlmt vlsnleailv rathsermta tyisdisldt avehqtgnrr avqvevcrcp
     2281 tghvgtsces cargyyrdpn drsasylgsc npcpcnnnee sceitrsgqv kchclpgytg
     2341 qyclstgelm vsikpmkgev manswilftc syefpeplyi sfkltpfggm pltatgvtpg
     2401 pimitekgaq rtwyvyvqle pcnvechiqd rdgkelikvv tsvtpetnvt tttpyppyip
     2461 pprilvyikg pefqiveagt tvryhcagks ldngslhikw ekeggqlptg rtvddsqgll
     2521 virdvkvsds gvyvcqvsdg vhigyknvtl tvggsnpvap ravvmptsle vvegqpaefr
     2581 cmatghpqpq vewirvhgpt spevvthngi wslraasknd aaeykciarn nvgvdeqtvi
     2641 lyvsdnpsmp pttgilptit psqwygtegd avlltcsqts pelaslvwlr qgnlpfspta
     2701 tqrdgvltim nstqydsgvy iclattyvgt hvnntaeitv vprrsppsik vrpekqtvpq
     2761 gsvaevqcvt sgepglqvkw skyhherlss nvqqvgdtlr ivniqipdrg vyvchvtgps
     2821 gshessavie vepreaprve iyprevppvi lggstdlqcr avagipmpel hwshqngrpf
     2881 psnikqlpgg vlrlsnitan dggayvcsav npvgsntavv yievqsvpvi misprsgilq
     2941 vkrgdrvkli crssgypqpr vawvkhqnri dfrtpfylss eaatpleavy einsaspdde
     3001 gsytchanna agmveervyi rvednevysp deypgpdkpe npddqkitvp kdyfkipsgg
     3061 kvemrcyvrs sdgnhiyldw krtdhrplpe gstvhngiln ipvadksaag eyvclgmdqa
     3121 rnvlfraksh levlsppriv lnpprqtvra genpsidcst tgdepmtiqw aaigrnlpss
     3181 vtqdrgvlqf hgitysdagk yvctatndag taeavaevlv nehpfdessv vhaaqrdvvt
     3241 yagqpvrlrc tvnvratihw srdgqplpst arieddylel prarpedsgr yicqiqtvhg
     3301 vssdyinlnv slvnlhtecs ranrtqcses kcicnvqgcf lldylcnvyi ryyndigdny
     3361 yntpqnrhyl qqrratvtpa vsveasqnpv nigdtvnirc acsgthnpry hwvrpnhpsl
     3421 penaqeygnv lrltnvavsd sgvyrciadt pegvfdhdfn lvvhdgnnda paietkyapy
     3481 gsslemdckp nlnpplkfhw sklggllprd aqifenklkl invkaedagt yvctatnnle
     3541 nieiptvlvv tgvvpyfsqa pesfialppl pdsylkfnie isfkpesydg iilyndesnr
     3601 gngdfillsl vggypvfsfd lgsgptiira eksvtlsewh tiklqrnrke gtmlvdgegp
     3661 fkgvaagrkq gldlkallfv ggvpsdntvn kyaeitsgfv gcisrlvige keidliddqt
     3721 nsvgitncet caenpcnnag vcqeaatkng ytclcragys gkhcdyvgqs cypgacgegt
     3781 cvdketgfec ycphgktgsr cehsltiyep afhddksfva hetpkalrrt rmnpsythnr
     3841 lrarnaqvrk arsrphdhnk mhhtkh
//
LOCUS       XP_034185295            3855 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034185295
VERSION     XP_034185295.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329404.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3855
                     /product="terribly reduced optic lobes isoform X8"
                     /calculated_mol_wt=425018
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          714..747
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(718,726,737..738)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(730,733,737,743..744)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            740..744
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          750..784
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(755,763,774..775)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(767,770,774,780..781)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            777..781
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          788..822
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(793,801,812..813)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(805,808,812,818..819)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            815..819
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          824..858
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(829,837,848..849)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(841,844,848,854..855)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            851..855
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          872..906
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(877,885,896..897)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(889,892,896,902..903)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            899..903
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          910..941
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(915,923,934..935)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(927,930,934,940..941)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            937..941
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          957..992
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(962,971,982..983)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(975,978,982,988..989)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            985..989
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1017..1093
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1023..1027
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1036..1040
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1056..1060
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1107..1141
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1112,1120,1131..1132)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1124,1127,1131,1137..1138)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1134..1138
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1146..1180
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1151,1159,1170..1171)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1163,1166,1170,1176..1177)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1173..1177
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1189..1224
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1194,1203,1214..1215)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1207,1210,1214,1220..1221)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1217..1221
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1242..1317
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1245..1249
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1258..1262
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1281..1285
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1295..1300
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1309..1312
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1419..1553
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1609..1662
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1610,1612,1625,1635,1637,1646)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1669..1715
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1670,1680,1687,1689,1698)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1786..1922
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1957..2005
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1957,1959,1966,1973,1976,1985)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2012..2058
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2012,2014,2024,2029,2031,2040)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2128..2264
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2460..2541
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2471..2475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2486..2490
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2507..2511
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2555..2632
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2566..2570
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2579..2583
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2598..2602
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2612..2617
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2625..2628
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2650..2729
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2661..2665
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2674..2678
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2694..2698
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2708..2713
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2721..2724
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2742..2820
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2753..2757
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2766..2770
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2785..2790
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2800..2805
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2813..2816
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2837..2913
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2844..2848
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2858..2862
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2879..2883
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2898
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2906..2909
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2928..3011
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2936..2940
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2949..2953
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2973..2981
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2991..2996
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3004..3007
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3035..3114
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3051..3055
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3065..3070
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3086..3090
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3100..3105
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3108..3111
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3126..3209
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3143..3147
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3156..3160
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3175..3179
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3189..3194
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3202..3205
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3224..3299
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3235..3239
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3246..3250
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3265..3269
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3279..3284
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3292..3295
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3379..3452
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3385..3389
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3398..3402
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3418..3422
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3432..3437
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3445..3448
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3470..3539
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3473..3477
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3486..3490
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3506..3509
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3519..3524
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3532..3535
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3545..3697
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3720..3753
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3855
                     /gene="trol"
                     /coded_by="XM_034329404.2:322..11889"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcgqp wpgratdslr ecdpsremrc
      721 ddgkcvllrr kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd
      781 eagcvnecpa gmfrcndglc ldskrrcdgr phcldgsdei ncqcpdgelp cdngvcinkn
      841 ffcdrnadch dgsderdcpv idettdgdhv acrqdefacp hggcipqsav cngrndcpye
      901 edeincprgc gkdqfqcsng dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag
      961 yimcvsdkdc vpqssmcngi pecrdrsdee nceqlkepsr lnlktypseq vikenpakqg
     1021 revvfqcrde gplrarvrwl rgnnlplppg srdlngrlei pniqldhggl yiceavgypa
     1081 sttgqqvnvy ltvekfeppa tnkphvcmyd eatcsngdci pksyvcngrl dctdgsdemr
     1141 csphgcepne frcnntqcvs klwrcdgdkd cadgsdeenc vpnrpgspcr vteytcasnd
     1201 qcipksyhcd mekdcidgsd eigcspvyim kppppmitld lgttlvivct aigvptpein
     1261 wrlnwghipp kctmtsingt gtltcpdiqt edqgayscea lntagfvfav pdaivmvnkp
     1321 ddvcpkgmfn seartveeci scfcfgvate chsanlftyq ipppfdrhki vavkqepdlr
     1381 ilndisyqla vkplgrdglh ldestpflnq laaekdvpyf alpenfhgsq lksyggylky
     1441 rvryngtgvp nsapavvlsg nnyvlihkgk qlipnydnde svrffegewl kkqgwsevpa
     1501 tredimmtla nvdnilikve ydnspdldvq lthivmdtad vrntglgsas fveecqcpsg
     1561 ytglsceeca pgylrrktgl wlgqcyrdep vdcppgyygd psrnipckac pcpltnpsnq
     1621 fartchlgsd gqptcncppg yigrrceqcd vgyqgnplip gdmcvarqqc dpegsissvv
     1681 dpltgkcrck qyatgitcnq ckantfknqf gctscfcmgi tnkcvssnwy rndvrvsftn
     1741 sirdfslies rtphpppivd girlntvtre iiyndfpnrg nndvyywqlp siflgdqvts
     1801 yggnlkytvr yvpapggqss knnaadveli sandinllyf srespepntp qtftvplleq
     1861 ywqrndgtqa drehllmala dvsairikat ytthtdeaal slvsldiaek yntgrarave
     1921 veecscpigy kglscedcdv gytrasegly lgiceqcncn ghsaqcnpdt gicencadht
     1981 tgdfcevcep gyegdatrgt phdcvykdst pcrcneagsr ssscigdrcd ckrnvegpqc
     2041 nrcrpstfgl ssynpdgcte cycsgvtnqc hesslyvqqi pvwvydnqhg ftltdstrqe
     2101 iiddgfelnf amneigyryp dsrgrrlfws lpaiftgnkv ksyggnltlt qhitaypgaq
     2161 sykdqdiili gngitlfwtn pmdiqpdipl tysvplrese wrrlttegpr vasridlmtv
     2221 lsnleailvr athsermtat yisdisldta vehqtgnrra vqvevcrcpt ghvgtscesc
     2281 argyyrdpnd rsasylgscn pcpcnnnees ceitrsgqvk chclpgytgq yclstgelmv
     2341 sikpmkgevm answilftcs yefpeplyis fkltpfggmp ltatgvtpgp imitekgaqr
     2401 twyvyvqlep cnvechiqdr dgkelikvvt svtpetnvtt ttpyppyipp prilvyikgp
     2461 efqiveagtt vryhcagksl dngslhikwe keggqlptgr tvddsqgllv irdvkvsdsg
     2521 vyvcqvsdgv higyknvtlt vggsnpvapr avvmptslev vegqpaefrc matghpqpqv
     2581 ewirvhgpts pevvthngiw slraasknda aeykciarnn vgvdeqtvil yvsdnpsmpp
     2641 ttgilptitp sqwygtegda vlltcsqtsp elaslvwlrq gnlpfsptat qrdgvltimn
     2701 stqydsgvyi clattyvgth vnntaeitvv prrsppsikv rpekqtvpqg svaevqcvts
     2761 gepglqvkws kyhherlssn vqqvgdtlri vniqipdrgv yvchvtgpsg shessaviev
     2821 epreaprvei yprevppvil ggstdlqcra vagipmpelh wshqngrpfp snikqlpggv
     2881 lrlsnitand ggayvcsavn pvgsntavvy ievqsvpvim isprsgilqv krgdrvklic
     2941 rssgypqprv awvkhqnrid frtpfylsse aatpleavye insaspddeg sytchannaa
     3001 gmveervyir vednevyspd eypgpdkpen pddqkitvpk dyfkipsggk vemrcyvrss
     3061 dgnhiyldwk rtdhrplpeg stvhngilni pvadksaage yvclgmdqar nvlfrakshl
     3121 evlspprivl npprqtvrag enpsidcstt gdepmtiqwa aigrnlpssv tqdrgvlqfh
     3181 gitysdagky vctatndagt aeavaevlvn ehpfdessvv haaqrdvvty agqpvrlrct
     3241 vnvratihws rdgqplpsta rieddylelp rarpedsgry icqiqtvhgv ssdyinlnvs
     3301 lvnlhtecsr anrtqcsesk cicnvqgcfl ldylcnvyir yyndigdnyy ntpqnrhylq
     3361 qrratvtpav sveasqnpvn igdtvnirca csgthnpryh wvrpnhpslp enaqeygnvl
     3421 rltnvavsds gvyrciadtp egvfdhdfnl vvhdgnndap aietkyapyg sslemdckpn
     3481 lnpplkfhws klggllprda qifenklkli nvkaedagty vctatnnlen ieiptvlvvt
     3541 gvvpyfsqap esfialpplp dsylkfniei sfkpesydgi ilyndesnrg ngdfillslv
     3601 ggypvfsfdl gsgptiirae ksvtlsewht iklqrnrkeg tmlvdgegpf kgvaagrkqg
     3661 ldlkallfvg gvpsdntvnk yaeitsgfvg cisrlvigek eidliddqtn svgitncetc
     3721 aenpcnnagv cqeaatkngy tclcragysg khcdyvgqsc ypgacgegtc vdketgfecy
     3781 cphgktgsrc ehsltiyepa fhddksfvah etpkalrrtr mnpsythnrl rarnaqvrka
     3841 rsrphdhnkm hhtkh
//
LOCUS       XP_034185296            3842 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034185296
VERSION     XP_034185296.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329405.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3842
                     /product="terribly reduced optic lobes isoform X9"
                     /calculated_mol_wt=423054
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          800..834
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(805,813,824..825)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(817,820,824,830..831)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            827..831
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          838..872
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(843,851,862..863)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(855,858,862,868..869)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            865..869
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          874..908
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(879,887,898..899)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(891,894,898,904..905)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            901..905
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          922..956
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(927,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          960..991
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(965,973,984..985)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(977,980,984,990..991)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            987..991
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1007..1042
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1012,1021,1032..1033)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1025,1028,1032,1038..1039)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1035..1039
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1067..1143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1073..1077
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1086..1090
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1106..1110
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1157..1191
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1162,1170,1181..1182)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1174,1177,1181,1187..1188)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1184..1188
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1196..1230
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1201,1209,1220..1221)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1213,1216,1220,1226..1227)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1223..1227
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1239..1274
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1244,1253,1264..1265)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1257,1260,1264,1270..1271)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1267..1271
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1292..1367
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1295..1299
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1308..1312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1331..1335
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1345..1350
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1359..1362
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1469..1603
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1659..1712
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1660,1662,1675,1685,1687,1696)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1719..1765
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1720,1730,1737,1739,1748)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1836..1972
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2007..2055
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2007,2009,2016,2023,2026,2035)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2062..2108
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2062,2064,2074,2079,2081,2090)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2178..2314
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2510..2591
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2521..2525
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2536..2540
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2557..2561
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2605..2682
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2616..2620
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2629..2633
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2648..2652
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2662..2667
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2675..2678
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2700..2779
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2711..2715
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2724..2728
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2744..2748
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2758..2763
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2771..2774
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2792..2870
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2803..2807
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2816..2820
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2835..2840
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2850..2855
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2863..2866
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2887..2963
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2894..2898
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2908..2912
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2929..2933
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2943..2948
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2956..2959
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2978..3061
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2986..2990
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2999..3003
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3023..3031
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3041..3046
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3054..3057
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3085..3164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3101..3105
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3115..3120
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3136..3140
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3150..3155
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3158..3161
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3176..3259
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3193..3197
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3206..3210
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3225..3229
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3239..3244
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3252..3255
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3349
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3285..3289
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3297..3301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3315..3319
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3329..3334
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3343..3346
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3366..3439
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3372..3376
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3385..3389
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3405..3409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3419..3424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3432..3435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3457..3526
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3460..3464
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3473..3477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3493..3496
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3506..3511
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3519..3522
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3532..3684
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3707..3740
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3842
                     /gene="trol"
                     /coded_by="XM_034329405.2:322..11850"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd eagcvnecpa
      841 gmfrcndglc ldskrrcdgr phcldgsdei ncqcpdgelp cdngvcinkn ffcdrnadch
      901 dgsderdcpv idettdgdhv acrqdefacp hggcipqsav cngrndcpye edeincprgc
      961 gkdqfqcsng dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag yimcvsdkdc
     1021 vpqssmcngi pecrdrsdee nceqlkepsr lnlktypseq vikenpakqg revvfqcrde
     1081 gplrarvrwl rgnnlplppg srdlngrlei pniqldhggl yiceavgypa sttgqqvnvy
     1141 ltvekfeppa tnkphvcmyd eatcsngdci pksyvcngrl dctdgsdemr csphgcepne
     1201 frcnntqcvs klwrcdgdkd cadgsdeenc vpnrpgspcr vteytcasnd qcipksyhcd
     1261 mekdcidgsd eigcspvyim kppppmitld lgttlvivct aigvptpein wrlnwghipp
     1321 kctmtsingt gtltcpdiqt edqgayscea lntagfvfav pdaivmvnkp ddvcpkgmfn
     1381 seartveeci scfcfgvate chsanlftyq ipppfdrhki vavkqepdlr ilndisyqla
     1441 vkplgrdglh ldestpflnq laaekdvpyf alpenfhgsq lksyggylky rvryngtgvp
     1501 nsapavvlsg nnyvlihkgk qlipnydnde svrffegewl kkqgwsevpa tredimmtla
     1561 nvdnilikve ydnspdldvq lthivmdtad vrntglgsas fveecqcpsg ytglsceeca
     1621 pgylrrktgl wlgqcyrdep vdcppgyygd psrnipckac pcpltnpsnq fartchlgsd
     1681 gqptcncppg yigrrceqcd vgyqgnplip gdmcvarqqc dpegsissvv dpltgkcrck
     1741 qyatgitcnq ckantfknqf gctscfcmgi tnkcvssnwy rndvrvsftn sirdfslies
     1801 rtphpppivd girlntvtre iiyndfpnrg nndvyywqlp siflgdqvts yggnlkytvr
     1861 yvpapggqss knnaadveli sandinllyf srespepntp qtftvplleq ywqrndgtqa
     1921 drehllmala dvsairikat ytthtdeaal slvsldiaek yntgrarave veecscpigy
     1981 kglscedcdv gytrasegly lgiceqcncn ghsaqcnpdt gicencadht tgdfcevcep
     2041 gyegdatrgt phdcvykdst pcrcneagsr ssscigdrcd ckrnvegpqc nrcrpstfgl
     2101 ssynpdgcte cycsgvtnqc hesslyvqqi pvwvydnqhg ftltdstrqe iiddgfelnf
     2161 amneigyryp dsrgrrlfws lpaiftgnkv ksyggnltlt qhitaypgaq sykdqdiili
     2221 gngitlfwtn pmdiqpdipl tysvplrese wrrlttegpr vasridlmtv lsnleailvr
     2281 athsermtat yisdisldta vehqtgnrra vqvevcrcpt ghvgtscesc argyyrdpnd
     2341 rsasylgscn pcpcnnnees ceitrsgqvk chclpgytgq yclstgelmv sikpmkgevm
     2401 answilftcs yefpeplyis fkltpfggmp ltatgvtpgp imitekgaqr twyvyvqlep
     2461 cnvechiqdr dgkelikvvt svtpetnvtt ttpyppyipp prilvyikgp efqiveagtt
     2521 vryhcagksl dngslhikwe keggqlptgr tvddsqgllv irdvkvsdsg vyvcqvsdgv
     2581 higyknvtlt vggsnpvapr avvmptslev vegqpaefrc matghpqpqv ewirvhgpts
     2641 pevvthngiw slraasknda aeykciarnn vgvdeqtvil yvsdnpsmpp ttgilptitp
     2701 sqwygtegda vlltcsqtsp elaslvwlrq gnlpfsptat qrdgvltimn stqydsgvyi
     2761 clattyvgth vnntaeitvv prrsppsikv rpekqtvpqg svaevqcvts gepglqvkws
     2821 kyhherlssn vqqvgdtlri vniqipdrgv yvchvtgpsg shessaviev epreaprvei
     2881 yprevppvil ggstdlqcra vagipmpelh wshqngrpfp snikqlpggv lrlsnitand
     2941 ggayvcsavn pvgsntavvy ievqsvpvim isprsgilqv krgdrvklic rssgypqprv
     3001 awvkhqnrid frtpfylsse aatpleavye insaspddeg sytchannaa gmveervyir
     3061 vednevyspd eypgpdkpen pddqkitvpk dyfkipsggk vemrcyvrss dgnhiyldwk
     3121 rtdhrplpeg stvhngilni pvadksaage yvclgmdqar nvlfrakshl evlspprivl
     3181 npprqtvrag enpsidcstt gdepmtiqwa aigrnlpssv tqdrgvlqfh gitysdagky
     3241 vctatndagt aeavaevlvn ehpfdessvv haaqrdvvty agqpvrlrct vnvratihws
     3301 rdgqplpsta rieddylelp rarpedsgry icqiqtvhgv ssdyinlnvs ptvtpavsve
     3361 asqnpvnigd tvnircacsg thnpryhwvr pnhpslpena qeygnvlrlt nvavsdsgvy
     3421 rciadtpegv fdhdfnlvvh dgnndapaie tkyapygssl emdckpnlnp plkfhwsklg
     3481 gllprdaqif enklklinvk aedagtyvct atnnleniei ptvlvvtgvv pyfsqapesf
     3541 ialpplpdsy lkfnieisfk pesydgiily ndesnrgngd fillslvggy pvfsfdlgsg
     3601 ptiiraeksv tlsewhtikl qrnrkegtml vdgegpfkgv aagrkqgldl kallfvggvp
     3661 sdntvnkyae itsgfvgcis rlvigekeid liddqtnsvg itncetcaen pcnnagvcqe
     3721 aatkngytcl cragysgkhc dyvgqscypg acgegtcvdk etgfecycph gktgsrcehs
     3781 ltiyepafhd dksfvahetp kalrrtrmnp sythnrlrar naqvrkarsr phdhnkmhht
     3841 kh
//
LOCUS       XP_034185297            3830 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_034185297
VERSION     XP_034185297.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329406.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3830
                     /product="terribly reduced optic lobes isoform X10"
                     /calculated_mol_wt=422596
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          625..658
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(631,640,651..652)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(644,647,651,657..658)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            654..658
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          689..722
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(693,701,712..713)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(705,708,712,718..719)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            715..719
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          725..759
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(730,738,749..750)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(742,745,749,755..756)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            752..756
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          763..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          799..833
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(804,812,823..824)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(816,819,823,829..830)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            826..830
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          847..881
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(852,860,871..872)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(864,867,871,877..878)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            874..878
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          885..916
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(890,898,909..910)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(902,905,909,915..916)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            912..916
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          932..967
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(937,946,957..958)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(950,953,957,963..964)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            960..964
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          992..1068
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          998..1002
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1011..1015
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1031..1035
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1082..1116
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1087,1095,1106..1107)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1099,1102,1106,1112..1113)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1109..1113
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1121..1155
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1126,1134,1145..1146)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1138,1141,1145,1151..1152)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1148..1152
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1164..1199
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1169,1178,1189..1190)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1182,1185,1189,1195..1196)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1192..1196
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1217..1292
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1220..1224
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1233..1237
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1256..1260
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1270..1275
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1284..1287
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1394..1528
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1584..1637
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1585,1587,1600,1610,1612,1621)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1644..1690
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1645,1655,1662,1664,1673)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1761..1897
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1932..1980
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1932,1934,1941,1948,1951,1960)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1987..2033
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1987,1989,1999,2004,2006,2015)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2103..2239
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2435..2516
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2446..2450
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2461..2465
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2482..2486
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2530..2607
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2541..2545
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2554..2558
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2573..2577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2587..2592
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2600..2603
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2625..2704
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2636..2640
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2649..2653
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2669..2673
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2683..2688
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2696..2699
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2717..2795
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2728..2732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2741..2745
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2760..2765
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2775..2780
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2788..2791
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2812..2888
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2819..2823
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2833..2837
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2854..2858
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2868..2873
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2881..2884
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2903..2986
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2911..2915
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2924..2928
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2948..2956
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2966..2971
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2979..2982
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3010..3089
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3026..3030
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3040..3045
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3061..3065
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3075..3080
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3083..3086
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3101..3184
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3118..3122
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3131..3135
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3150..3154
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3164..3169
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3177..3180
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3199..3274
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3210..3214
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3221..3225
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3240..3244
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3254..3259
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3267..3270
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3354..3427
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3360..3364
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3373..3377
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3393..3397
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3407..3412
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3420..3423
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3445..3514
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3448..3452
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3461..3465
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3481..3484
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3494..3499
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3507..3510
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3520..3672
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3695..3728
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3830
                     /gene="trol"
                     /coded_by="XM_034329406.2:322..11814"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcerectptq fkcvtgnkci egvyrcdghp dcpdhsdedc
      661 anetithaiq ptsqpwpgra tdslrecdps remrcddgkc vllrrkcdni fdcidgsder
      721 gcgvctpaew kcasgecipe tercdnvvqc adgtdeagcv necpagmfrc ndglcldskr
      781 rcdgrphcld gsdeincqcp dgelpcdngv cinknffcdr nadchdgsde rdcpvidett
      841 dgdhvacrqd efacphggci pqsavcngrn dcpyeedein cprgcgkdqf qcsngdcira
      901 dqkcngyidc vdgsdepeec erpgfhpmpg rcpagyimcv sdkdcvpqss mcngipecrd
      961 rsdeenceql kepsrlnlkt ypseqviken pakqgrevvf qcrdegplra rvrwlrgnnl
     1021 plppgsrdln grleipniql dhgglyicea vgypasttgq qvnvyltvek feppatnkph
     1081 vcmydeatcs ngdcipksyv cngrldctdg sdemrcsphg cepnefrcnn tqcvsklwrc
     1141 dgdkdcadgs deencvpnrp gspcrvteyt casndqcipk syhcdmekdc idgsdeigcs
     1201 pvyimkpppp mitldlgttl vivctaigvp tpeinwrlnw ghippkctmt singtgtltc
     1261 pdiqtedqga yscealntag fvfavpdaiv mvnkpddvcp kgmfnseart veeciscfcf
     1321 gvatechsan lftyqipppf drhkivavkq epdlrilndi syqlavkplg rdglhldest
     1381 pflnqlaaek dvpyfalpen fhgsqlksyg gylkyrvryn gtgvpnsapa vvlsgnnyvl
     1441 ihkgkqlipn ydndesvrff egewlkkqgw sevpatredi mmtlanvdni likveydnsp
     1501 dldvqlthiv mdtadvrntg lgsasfveec qcpsgytgls ceecapgylr rktglwlgqc
     1561 yrdepvdcpp gyygdpsrni pckacpcplt npsnqfartc hlgsdgqptc ncppgyigrr
     1621 ceqcdvgyqg nplipgdmcv arqqcdpegs issvvdpltg kcrckqyatg itcnqckant
     1681 fknqfgctsc fcmgitnkcv ssnwyrndvr vsftnsirdf sliesrtphp ppivdgirln
     1741 tvtreiiynd fpnrgnndvy ywqlpsiflg dqvtsyggnl kytvryvpap ggqssknnaa
     1801 dvelisandi nllyfsresp epntpqtftv plleqywqrn dgtqadrehl lmaladvsai
     1861 rikatyttht deaalslvsl diaekyntgr araveveecs cpigykglsc edcdvgytra
     1921 seglylgice qcncnghsaq cnpdtgicen cadhttgdfc evcepgyegd atrgtphdcv
     1981 ykdstpcrcn eagsrsssci gdrcdckrnv egpqcnrcrp stfglssynp dgctecycsg
     2041 vtnqchessl yvqqipvwvy dnqhgftltd strqeiiddg felnfamnei gyrypdsrgr
     2101 rlfwslpaif tgnkvksygg nltltqhita ypgaqsykdq diiligngit lfwtnpmdiq
     2161 pdipltysvp lresewrrlt tegprvasri dlmtvlsnle ailvrathse rmtatyisdi
     2221 sldtavehqt gnrravqvev crcptghvgt scescargyy rdpndrsasy lgscnpcpcn
     2281 nneesceitr sgqvkchclp gytgqyclst gelmvsikpm kgevmanswi lftcsyefpe
     2341 plyisfkltp fggmpltatg vtpgpimite kgaqrtwyvy vqlepcnvec hiqdrdgkel
     2401 ikvvtsvtpe tnvttttpyp pyippprilv yikgpefqiv eagttvryhc agksldngsl
     2461 hikwekeggq lptgrtvdds qgllvirdvk vsdsgvyvcq vsdgvhigyk nvtltvggsn
     2521 pvapravvmp tslevvegqp aefrcmatgh pqpqvewirv hgptspevvt hngiwslraa
     2581 skndaaeykc iarnnvgvde qtvilyvsdn psmppttgil ptitpsqwyg tegdavlltc
     2641 sqtspelasl vwlrqgnlpf sptatqrdgv ltimnstqyd sgvyiclatt yvgthvnnta
     2701 eitvvprrsp psikvrpekq tvpqgsvaev qcvtsgepgl qvkwskyhhe rlssnvqqvg
     2761 dtlrivniqi pdrgvyvchv tgpsgshess avieveprea prveiyprev ppvilggstd
     2821 lqcravagip mpelhwshqn grpfpsnikq lpggvlrlsn itandggayv csavnpvgsn
     2881 tavvyievqs vpvimisprs gilqvkrgdr vklicrssgy pqprvawvkh qnridfrtpf
     2941 ylsseaatpl eavyeinsas pddegsytch annaagmvee rvyirvedne vyspdeypgp
     3001 dkpenpddqk itvpkdyfki psggkvemrc yvrssdgnhi yldwkrtdhr plpegstvhn
     3061 gilnipvadk saageyvclg mdqarnvlfr akshlevlsp privlnpprq tvragenpsi
     3121 dcsttgdepm tiqwaaigrn lpssvtqdrg vlqfhgitys dagkyvctat ndagtaeava
     3181 evlvnehpfd essvvhaaqr dvvtyagqpv rlrctvnvra tihwsrdgqp lpstariedd
     3241 ylelprarpe dsgryicqiq tvhgvssdyi nlnvslvnlh tecsranrtq cseskcicnv
     3301 qgcflldylc nvyiryyndi gdnyyntpqn rhylqqrrat vtpavsveas qnpvnigdtv
     3361 nircacsgth npryhwvrpn hpslpenaqe ygnvlrltnv avsdsgvyrc iadtpegvfd
     3421 hdfnlvvhdg nndapaietk yapygsslem dckpnlnppl kfhwsklggl lprdaqifen
     3481 klklinvkae dagtyvctat nnlenieipt vlvvtgvvpy fsqapesfia lpplpdsylk
     3541 fnieisfkpe sydgiilynd esnrgngdfi llslvggypv fsfdlgsgpt iiraeksvtl
     3601 sewhtiklqr nrkegtmlvd gegpfkgvaa grkqgldlka llfvggvpsd ntvnkyaeit
     3661 sgfvgcisrl vigekeidli ddqtnsvgit ncetcaenpc nnagvcqeaa tkngytclcr
     3721 agysgkhcdy vgqscypgac gegtcvdket gfecycphgk tgsrcehslt iyepafhddk
     3781 sfvahetpka lrrtrmnpsy thnrlrarna qvrkarsrph dhnkmhhtkh
//
LOCUS       XP_034185298            3830 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_034185298
VERSION     XP_034185298.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329407.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3830
                     /product="terribly reduced optic lobes isoform X11"
                     /calculated_mol_wt=422584
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          799..833
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(804,812,823..824)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(816,819,823,829..830)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            826..830
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          847..881
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(852,860,871..872)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(864,867,871,877..878)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            874..878
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          885..916
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(890,898,909..910)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(902,905,909,915..916)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            912..916
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          932..967
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(937,946,957..958)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(950,953,957,963..964)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            960..964
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          992..1068
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          998..1002
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1011..1015
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1031..1035
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1082..1116
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1087,1095,1106..1107)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1099,1102,1106,1112..1113)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1109..1113
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1121..1155
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1126,1134,1145..1146)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1138,1141,1145,1151..1152)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1148..1152
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1164..1199
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1169,1178,1189..1190)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1182,1185,1189,1195..1196)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1192..1196
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1217..1292
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1220..1224
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1233..1237
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1256..1260
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1270..1275
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1284..1287
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1394..1528
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1584..1637
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1585,1587,1600,1610,1612,1621)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1644..1690
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1645,1655,1662,1664,1673)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1761..1897
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1932..1980
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1932,1934,1941,1948,1951,1960)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1987..2033
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1987,1989,1999,2004,2006,2015)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2103..2239
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2435..2516
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2446..2450
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2461..2465
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2482..2486
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2530..2607
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2541..2545
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2554..2558
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2573..2577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2587..2592
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2600..2603
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2625..2704
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2636..2640
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2649..2653
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2669..2673
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2683..2688
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2696..2699
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2717..2795
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2728..2732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2741..2745
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2760..2765
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2775..2780
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2788..2791
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2812..2888
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2819..2823
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2833..2837
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2854..2858
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2868..2873
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2881..2884
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2903..2986
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2911..2915
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2924..2928
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2948..2956
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2966..2971
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2979..2982
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3010..3089
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3026..3030
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3040..3045
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3061..3065
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3075..3080
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3083..3086
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3101..3184
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3118..3122
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3131..3135
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3150..3154
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3164..3169
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3177..3180
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3199..3274
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3210..3214
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3221..3225
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3240..3244
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3254..3259
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3267..3270
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3354..3427
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3360..3364
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3373..3377
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3393..3397
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3407..3412
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3420..3423
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3445..3514
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3448..3452
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3461..3465
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3481..3484
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3494..3499
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3507..3510
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3520..3672
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3695..3728
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3830
                     /gene="trol"
                     /coded_by="XM_034329407.2:322..11814"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcecp dgelpcdngv cinknffcdr nadchdgsde rdcpvidett
      841 dgdhvacrqd efacphggci pqsavcngrn dcpyeedein cprgcgkdqf qcsngdcira
      901 dqkcngyidc vdgsdepeec erpgfhpmpg rcpagyimcv sdkdcvpqss mcngipecrd
      961 rsdeenceql kepsrlnlkt ypseqviken pakqgrevvf qcrdegplra rvrwlrgnnl
     1021 plppgsrdln grleipniql dhgglyicea vgypasttgq qvnvyltvek feppatnkph
     1081 vcmydeatcs ngdcipksyv cngrldctdg sdemrcsphg cepnefrcnn tqcvsklwrc
     1141 dgdkdcadgs deencvpnrp gspcrvteyt casndqcipk syhcdmekdc idgsdeigcs
     1201 pvyimkpppp mitldlgttl vivctaigvp tpeinwrlnw ghippkctmt singtgtltc
     1261 pdiqtedqga yscealntag fvfavpdaiv mvnkpddvcp kgmfnseart veeciscfcf
     1321 gvatechsan lftyqipppf drhkivavkq epdlrilndi syqlavkplg rdglhldest
     1381 pflnqlaaek dvpyfalpen fhgsqlksyg gylkyrvryn gtgvpnsapa vvlsgnnyvl
     1441 ihkgkqlipn ydndesvrff egewlkkqgw sevpatredi mmtlanvdni likveydnsp
     1501 dldvqlthiv mdtadvrntg lgsasfveec qcpsgytgls ceecapgylr rktglwlgqc
     1561 yrdepvdcpp gyygdpsrni pckacpcplt npsnqfartc hlgsdgqptc ncppgyigrr
     1621 ceqcdvgyqg nplipgdmcv arqqcdpegs issvvdpltg kcrckqyatg itcnqckant
     1681 fknqfgctsc fcmgitnkcv ssnwyrndvr vsftnsirdf sliesrtphp ppivdgirln
     1741 tvtreiiynd fpnrgnndvy ywqlpsiflg dqvtsyggnl kytvryvpap ggqssknnaa
     1801 dvelisandi nllyfsresp epntpqtftv plleqywqrn dgtqadrehl lmaladvsai
     1861 rikatyttht deaalslvsl diaekyntgr araveveecs cpigykglsc edcdvgytra
     1921 seglylgice qcncnghsaq cnpdtgicen cadhttgdfc evcepgyegd atrgtphdcv
     1981 ykdstpcrcn eagsrsssci gdrcdckrnv egpqcnrcrp stfglssynp dgctecycsg
     2041 vtnqchessl yvqqipvwvy dnqhgftltd strqeiiddg felnfamnei gyrypdsrgr
     2101 rlfwslpaif tgnkvksygg nltltqhita ypgaqsykdq diiligngit lfwtnpmdiq
     2161 pdipltysvp lresewrrlt tegprvasri dlmtvlsnle ailvrathse rmtatyisdi
     2221 sldtavehqt gnrravqvev crcptghvgt scescargyy rdpndrsasy lgscnpcpcn
     2281 nneesceitr sgqvkchclp gytgqyclst gelmvsikpm kgevmanswi lftcsyefpe
     2341 plyisfkltp fggmpltatg vtpgpimite kgaqrtwyvy vqlepcnvec hiqdrdgkel
     2401 ikvvtsvtpe tnvttttpyp pyippprilv yikgpefqiv eagttvryhc agksldngsl
     2461 hikwekeggq lptgrtvdds qgllvirdvk vsdsgvyvcq vsdgvhigyk nvtltvggsn
     2521 pvapravvmp tslevvegqp aefrcmatgh pqpqvewirv hgptspevvt hngiwslraa
     2581 skndaaeykc iarnnvgvde qtvilyvsdn psmppttgil ptitpsqwyg tegdavlltc
     2641 sqtspelasl vwlrqgnlpf sptatqrdgv ltimnstqyd sgvyiclatt yvgthvnnta
     2701 eitvvprrsp psikvrpekq tvpqgsvaev qcvtsgepgl qvkwskyhhe rlssnvqqvg
     2761 dtlrivniqi pdrgvyvchv tgpsgshess avieveprea prveiyprev ppvilggstd
     2821 lqcravagip mpelhwshqn grpfpsnikq lpggvlrlsn itandggayv csavnpvgsn
     2881 tavvyievqs vpvimisprs gilqvkrgdr vklicrssgy pqprvawvkh qnridfrtpf
     2941 ylsseaatpl eavyeinsas pddegsytch annaagmvee rvyirvedne vyspdeypgp
     3001 dkpenpddqk itvpkdyfki psggkvemrc yvrssdgnhi yldwkrtdhr plpegstvhn
     3061 gilnipvadk saageyvclg mdqarnvlfr akshlevlsp privlnpprq tvragenpsi
     3121 dcsttgdepm tiqwaaigrn lpssvtqdrg vlqfhgitys dagkyvctat ndagtaeava
     3181 evlvnehpfd essvvhaaqr dvvtyagqpv rlrctvnvra tihwsrdgqp lpstariedd
     3241 ylelprarpe dsgryicqiq tvhgvssdyi nlnvslvnlh tecsranrtq cseskcicnv
     3301 qgcflldylc nvyiryyndi gdnyyntpqn rhylqqrrat vtpavsveas qnpvnigdtv
     3361 nircacsgth npryhwvrpn hpslpenaqe ygnvlrltnv avsdsgvyrc iadtpegvfd
     3421 hdfnlvvhdg nndapaietk yapygsslem dckpnlnppl kfhwsklggl lprdaqifen
     3481 klklinvkae dagtyvctat nnlenieipt vlvvtgvvpy fsqapesfia lpplpdsylk
     3541 fnieisfkpe sydgiilynd esnrgngdfi llslvggypv fsfdlgsgpt iiraeksvtl
     3601 sewhtiklqr nrkegtmlvd gegpfkgvaa grkqgldlka llfvggvpsd ntvnkyaeit
     3661 sgfvgcisrl vigekeidli ddqtnsvgit ncetcaenpc nnagvcqeaa tkngytclcr
     3721 agysgkhcdy vgqscypgac gegtcvdket gfecycphgk tgsrcehslt iyepafhddk
     3781 sfvahetpka lrrtrmnpsy thnrlrarna qvrkarsrph dhnkmhhtkh
//
LOCUS       XP_034185299            3819 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X12 [Osmia lignaria lignaria].
ACCESSION   XP_034185299
VERSION     XP_034185299.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329408.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3819
                     /product="terribly reduced optic lobes isoform X12"
                     /calculated_mol_wt=421157
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          627..661
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(632,640,651..652)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(644,647,651,657..658)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            654..658
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          678..711
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(682,690,701..702)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(694,697,701,707..708)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            704..708
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          714..748
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(719,727,738..739)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(731,734,738,744..745)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            741..745
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          752..786
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(757,765,776..777)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(769,772,776,782..783)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            779..783
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          788..822
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(793,801,812..813)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(805,808,812,818..819)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            815..819
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          836..870
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(841,849,860..861)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(853,856,860,866..867)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            863..867
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          874..905
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(879,887,898..899)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(891,894,898,904..905)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            901..905
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          921..956
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(926,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          981..1057
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          987..991
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1000..1004
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1020..1024
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1071..1105
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1076,1084,1095..1096)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1088,1091,1095,1101..1102)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1098..1102
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1110..1144
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1115,1123,1134..1135)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1127,1130,1134,1140..1141)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1137..1141
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1153..1188
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1158,1167,1178..1179)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1171,1174,1178,1184..1185)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1181..1185
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1206..1281
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1209..1213
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1222..1226
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1245..1249
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1259..1264
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1273..1276
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1383..1517
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1573..1626
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1574,1576,1589,1599,1601,1610)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1633..1679
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1634,1644,1651,1653,1662)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1750..1886
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1921..1969
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1921,1923,1930,1937,1940,1949)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1976..2022
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1976,1978,1988,1993,1995,2004)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2092..2228
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2424..2505
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2435..2439
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2450..2454
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2471..2475
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2519..2596
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2530..2534
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2543..2547
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2562..2566
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2576..2581
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2589..2592
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2614..2693
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2625..2629
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2658..2662
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2672..2677
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2685..2688
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2706..2784
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2717..2721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2730..2734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2749..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2801..2877
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2808..2812
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2822..2826
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2843..2847
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2857..2862
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2870..2873
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2892..2975
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2900..2904
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2913..2917
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2937..2945
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2955..2960
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2968..2971
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2999..3078
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3015..3019
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3029..3034
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3050..3054
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3064..3069
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3072..3075
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3090..3173
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3107..3111
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3120..3124
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3139..3143
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3153..3158
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3166..3169
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3188..3263
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3199..3203
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3210..3214
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3229..3233
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3243..3248
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3256..3259
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3343..3416
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3349..3353
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3362..3366
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3382..3386
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3396..3401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3409..3412
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3434..3503
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3437..3441
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3450..3454
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3470..3473
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3483..3488
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3496..3499
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3509..3661
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3684..3717
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3819
                     /gene="trol"
                     /coded_by="XM_034329408.2:322..11781"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcdlapcpsm dftcsdgsci skssvcdgfd dcpraedelh
      661 cgqpwpgrat dslrecdpsr emrcddgkcv llrrkcdnif dcidgsderg cgvctpaewk
      721 casgecipet ercdnvvqca dgtdeagcvn ecpagmfrcn dglcldskrr cdgrphcldg
      781 sdeincqcpd gelpcdngvc inknffcdrn adchdgsder dcpvidettd gdhvacrqde
      841 facphggcip qsavcngrnd cpyeedeinc prgcgkdqfq csngdcirad qkcngyidcv
      901 dgsdepeece rpgfhpmpgr cpagyimcvs dkdcvpqssm cngipecrdr sdeenceqlk
      961 epsrlnlkty pseqvikenp akqgrevvfq crdegplrar vrwlrgnnlp lppgsrdlng
     1021 rleipniqld hgglyiceav gypasttgqq vnvyltvekf eppatnkphv cmydeatcsn
     1081 gdcipksyvc ngrldctdgs demrcsphgc epnefrcnnt qcvsklwrcd gdkdcadgsd
     1141 eencvpnrpg spcrvteytc asndqcipks yhcdmekdci dgsdeigcsp vyimkppppm
     1201 itldlgttlv ivctaigvpt peinwrlnwg hippkctmts ingtgtltcp diqtedqgay
     1261 scealntagf vfavpdaivm vnkpddvcpk gmfnseartv eeciscfcfg vatechsanl
     1321 ftyqipppfd rhkivavkqe pdlrilndis yqlavkplgr dglhldestp flnqlaaekd
     1381 vpyfalpenf hgsqlksygg ylkyrvryng tgvpnsapav vlsgnnyvli hkgkqlipny
     1441 dndesvrffe gewlkkqgws evpatredim mtlanvdnil ikveydnspd ldvqlthivm
     1501 dtadvrntgl gsasfveecq cpsgytglsc eecapgylrr ktglwlgqcy rdepvdcppg
     1561 yygdpsrnip ckacpcpltn psnqfartch lgsdgqptcn cppgyigrrc eqcdvgyqgn
     1621 plipgdmcva rqqcdpegsi ssvvdpltgk crckqyatgi tcnqckantf knqfgctscf
     1681 cmgitnkcvs snwyrndvrv sftnsirdfs liesrtphpp pivdgirlnt vtreiiyndf
     1741 pnrgnndvyy wqlpsiflgd qvtsyggnlk ytvryvpapg gqssknnaad velisandin
     1801 llyfsrespe pntpqtftvp lleqywqrnd gtqadrehll maladvsair ikatytthtd
     1861 eaalslvsld iaekyntgra raveveecsc pigykglsce dcdvgytras eglylgiceq
     1921 cncnghsaqc npdtgicenc adhttgdfce vcepgyegda trgtphdcvy kdstpcrcne
     1981 agsrssscig drcdckrnve gpqcnrcrps tfglssynpd gctecycsgv tnqchessly
     2041 vqqipvwvyd nqhgftltds trqeiiddgf elnfamneig yrypdsrgrr lfwslpaift
     2101 gnkvksyggn ltltqhitay pgaqsykdqd iiligngitl fwtnpmdiqp dipltysvpl
     2161 resewrrltt egprvasrid lmtvlsnlea ilvrathser mtatyisdis ldtavehqtg
     2221 nrravqvevc rcptghvgts cescargyyr dpndrsasyl gscnpcpcnn neesceitrs
     2281 gqvkchclpg ytgqyclstg elmvsikpmk gevmanswil ftcsyefpep lyisfkltpf
     2341 ggmpltatgv tpgpimitek gaqrtwyvyv qlepcnvech iqdrdgkeli kvvtsvtpet
     2401 nvttttpypp yippprilvy ikgpefqive agttvryhca gksldngslh ikwekeggql
     2461 ptgrtvddsq gllvirdvkv sdsgvyvcqv sdgvhigykn vtltvggsnp vapravvmpt
     2521 slevvegqpa efrcmatghp qpqvewirvh gptspevvth ngiwslraas kndaaeykci
     2581 arnnvgvdeq tvilyvsdnp smppttgilp titpsqwygt egdavlltcs qtspelaslv
     2641 wlrqgnlpfs ptatqrdgvl timnstqyds gvyiclatty vgthvnntae itvvprrspp
     2701 sikvrpekqt vpqgsvaevq cvtsgepglq vkwskyhher lssnvqqvgd tlrivniqip
     2761 drgvyvchvt gpsgshessa vievepreap rveiyprevp pvilggstdl qcravagipm
     2821 pelhwshqng rpfpsnikql pggvlrlsni tandggayvc savnpvgsnt avvyievqsv
     2881 pvimisprsg ilqvkrgdrv klicrssgyp qprvawvkhq nridfrtpfy lsseaatple
     2941 avyeinsasp ddegsytcha nnaagmveer vyirvednev yspdeypgpd kpenpddqki
     3001 tvpkdyfkip sggkvemrcy vrssdgnhiy ldwkrtdhrp lpegstvhng ilnipvadks
     3061 aageyvclgm dqarnvlfra kshlevlspp rivlnpprqt vragenpsid csttgdepmt
     3121 iqwaaigrnl pssvtqdrgv lqfhgitysd agkyvctatn dagtaeavae vlvnehpfde
     3181 ssvvhaaqrd vvtyagqpvr lrctvnvrat ihwsrdgqpl pstarieddy lelprarped
     3241 sgryicqiqt vhgvssdyin lnvslvnlht ecsranrtqc seskcicnvq gcflldylcn
     3301 vyiryyndig dnyyntpqnr hylqqrratv tpavsveasq npvnigdtvn ircacsgthn
     3361 pryhwvrpnh pslpenaqey gnvlrltnva vsdsgvyrci adtpegvfdh dfnlvvhdgn
     3421 ndapaietky apygsslemd ckpnlnpplk fhwsklggll prdaqifenk lklinvkaed
     3481 agtyvctatn nlenieiptv lvvtgvvpyf sqapesfial pplpdsylkf nieisfkpes
     3541 ydgiilynde snrgngdfil lslvggypvf sfdlgsgpti iraeksvtls ewhtiklqrn
     3601 rkegtmlvdg egpfkgvaag rkqgldlkal lfvggvpsdn tvnkyaeits gfvgcisrlv
     3661 igekeidlid dqtnsvgitn cetcaenpcn nagvcqeaat kngytclcra gysgkhcdyv
     3721 gqscypgacg egtcvdketg fecycphgkt gsrcehslti yepafhddks fvahetpkal
     3781 rrtrmnpsyt hnrlrarnaq vrkarsrphd hnkmhhtkh
//
LOCUS       XP_034185300            3816 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X13 [Osmia lignaria lignaria].
ACCESSION   XP_034185300
VERSION     XP_034185300.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329409.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3816
                     /product="terribly reduced optic lobes isoform X13"
                     /calculated_mol_wt=420878
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          675..708
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(679,687,698..699)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(691,694,698,704..705)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            701..705
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          711..745
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(716,724,735..736)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(728,731,735,741..742)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            738..742
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          749..783
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(754,762,773..774)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(766,769,773,779..780)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            776..780
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          785..819
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(790,798,809..810)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(802,805,809,815..816)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            812..816
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          833..867
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(838,846,857..858)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(850,853,857,863..864)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            860..864
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          871..902
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(876,884,895..896)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(888,891,895,901..902)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            898..902
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          918..953
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(923,932,943..944)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(936,939,943,949..950)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            946..950
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          978..1054
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          984..988
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          997..1001
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1017..1021
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1068..1102
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1073,1081,1092..1093)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1085,1088,1092,1098..1099)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1095..1099
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1107..1141
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1112,1120,1131..1132)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1124,1127,1131,1137..1138)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1134..1138
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1150..1185
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1155,1164,1175..1176)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1168,1171,1175,1181..1182)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1178..1182
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1203..1278
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1206..1210
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1219..1223
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1242..1246
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1256..1261
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1270..1273
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1380..1514
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1570..1623
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1571,1573,1586,1596,1598,1607)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1630..1676
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1631,1641,1648,1650,1659)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1747..1883
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1918..1966
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1918,1920,1927,1934,1937,1946)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1973..2019
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1973,1975,1985,1990,1992,2001)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2089..2225
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2421..2502
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2432..2436
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2447..2451
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2468..2472
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2516..2593
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2527..2531
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2540..2544
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2559..2563
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2573..2578
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2586..2589
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2611..2690
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2622..2626
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2635..2639
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2655..2659
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2669..2674
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2682..2685
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2703..2781
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2714..2718
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2727..2731
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2746..2751
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2761..2766
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2774..2777
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2798..2874
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2805..2809
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2819..2823
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2854..2859
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2867..2870
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2889..2972
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2897..2901
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2910..2914
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2934..2942
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2957
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2965..2968
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2996..3075
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3012..3016
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3026..3031
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3047..3051
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3061..3066
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3069..3072
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3087..3170
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3104..3108
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3117..3121
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3136..3140
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3150..3155
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3163..3166
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3185..3260
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3196..3200
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3207..3211
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3226..3230
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3240..3245
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3253..3256
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3340..3413
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3346..3350
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3359..3363
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3379..3383
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3393..3398
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3406..3409
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3431..3500
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3434..3438
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3447..3451
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3467..3470
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3480..3485
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3493..3496
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3506..3658
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3681..3714
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3816
                     /gene="trol"
                     /coded_by="XM_034329409.2:322..11772"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcgq
      661 pwpgratdsl recdpsremr cddgkcvllr rkcdnifdci dgsdergcgv ctpaewkcas
      721 gecipeterc dnvvqcadgt deagcvnecp agmfrcndgl cldskrrcdg rphcldgsde
      781 incqcpdgel pcdngvcink nffcdrnadc hdgsderdcp videttdgdh vacrqdefac
      841 phggcipqsa vcngrndcpy eedeincprg cgkdqfqcsn gdciradqkc ngyidcvdgs
      901 depeecerpg fhpmpgrcpa gyimcvsdkd cvpqssmcng ipecrdrsde enceqlkeps
      961 rlnlktypse qvikenpakq grevvfqcrd egplrarvrw lrgnnlplpp gsrdlngrle
     1021 ipniqldhgg lyiceavgyp asttgqqvnv yltvekfepp atnkphvcmy deatcsngdc
     1081 ipksyvcngr ldctdgsdem rcsphgcepn efrcnntqcv sklwrcdgdk dcadgsdeen
     1141 cvpnrpgspc rvteytcasn dqcipksyhc dmekdcidgs deigcspvyi mkppppmitl
     1201 dlgttlvivc taigvptpei nwrlnwghip pkctmtsing tgtltcpdiq tedqgaysce
     1261 alntagfvfa vpdaivmvnk pddvcpkgmf nseartveec iscfcfgvat echsanlfty
     1321 qipppfdrhk ivavkqepdl rilndisyql avkplgrdgl hldestpfln qlaaekdvpy
     1381 falpenfhgs qlksyggylk yrvryngtgv pnsapavvls gnnyvlihkg kqlipnydnd
     1441 esvrffegew lkkqgwsevp atredimmtl anvdnilikv eydnspdldv qlthivmdta
     1501 dvrntglgsa sfveecqcps gytglsceec apgylrrktg lwlgqcyrde pvdcppgyyg
     1561 dpsrnipcka cpcpltnpsn qfartchlgs dgqptcncpp gyigrrceqc dvgyqgnpli
     1621 pgdmcvarqq cdpegsissv vdpltgkcrc kqyatgitcn qckantfknq fgctscfcmg
     1681 itnkcvssnw yrndvrvsft nsirdfslie srtphpppiv dgirlntvtr eiiyndfpnr
     1741 gnndvyywql psiflgdqvt syggnlkytv ryvpapggqs sknnaadvel isandinlly
     1801 fsrespepnt pqtftvplle qywqrndgtq adrehllmal advsairika tytthtdeaa
     1861 lslvsldiae kyntgrarav eveecscpig ykglscedcd vgytrasegl ylgiceqcnc
     1921 nghsaqcnpd tgicencadh ttgdfcevce pgyegdatrg tphdcvykds tpcrcneags
     1981 rssscigdrc dckrnvegpq cnrcrpstfg lssynpdgct ecycsgvtnq chesslyvqq
     2041 ipvwvydnqh gftltdstrq eiiddgfeln famneigyry pdsrgrrlfw slpaiftgnk
     2101 vksyggnltl tqhitaypga qsykdqdiil igngitlfwt npmdiqpdip ltysvplres
     2161 ewrrlttegp rvasridlmt vlsnleailv rathsermta tyisdisldt avehqtgnrr
     2221 avqvevcrcp tghvgtsces cargyyrdpn drsasylgsc npcpcnnnee sceitrsgqv
     2281 kchclpgytg qyclstgelm vsikpmkgev manswilftc syefpeplyi sfkltpfggm
     2341 pltatgvtpg pimitekgaq rtwyvyvqle pcnvechiqd rdgkelikvv tsvtpetnvt
     2401 tttpyppyip pprilvyikg pefqiveagt tvryhcagks ldngslhikw ekeggqlptg
     2461 rtvddsqgll virdvkvsds gvyvcqvsdg vhigyknvtl tvggsnpvap ravvmptsle
     2521 vvegqpaefr cmatghpqpq vewirvhgpt spevvthngi wslraasknd aaeykciarn
     2581 nvgvdeqtvi lyvsdnpsmp pttgilptit psqwygtegd avlltcsqts pelaslvwlr
     2641 qgnlpfspta tqrdgvltim nstqydsgvy iclattyvgt hvnntaeitv vprrsppsik
     2701 vrpekqtvpq gsvaevqcvt sgepglqvkw skyhherlss nvqqvgdtlr ivniqipdrg
     2761 vyvchvtgps gshessavie vepreaprve iyprevppvi lggstdlqcr avagipmpel
     2821 hwshqngrpf psnikqlpgg vlrlsnitan dggayvcsav npvgsntavv yievqsvpvi
     2881 misprsgilq vkrgdrvkli crssgypqpr vawvkhqnri dfrtpfylss eaatpleavy
     2941 einsaspdde gsytchanna agmveervyi rvednevysp deypgpdkpe npddqkitvp
     3001 kdyfkipsgg kvemrcyvrs sdgnhiyldw krtdhrplpe gstvhngiln ipvadksaag
     3061 eyvclgmdqa rnvlfraksh levlsppriv lnpprqtvra genpsidcst tgdepmtiqw
     3121 aaigrnlpss vtqdrgvlqf hgitysdagk yvctatndag taeavaevlv nehpfdessv
     3181 vhaaqrdvvt yagqpvrlrc tvnvratihw srdgqplpst arieddylel prarpedsgr
     3241 yicqiqtvhg vssdyinlnv slvnlhtecs ranrtqcses kcicnvqgcf lldylcnvyi
     3301 ryyndigdny yntpqnrhyl qqrratvtpa vsveasqnpv nigdtvnirc acsgthnpry
     3361 hwvrpnhpsl penaqeygnv lrltnvavsd sgvyrciadt pegvfdhdfn lvvhdgnnda
     3421 paietkyapy gsslemdckp nlnpplkfhw sklggllprd aqifenklkl invkaedagt
     3481 yvctatnnle nieiptvlvv tgvvpyfsqa pesfialppl pdsylkfnie isfkpesydg
     3541 iilyndesnr gngdfillsl vggypvfsfd lgsgptiira eksvtlsewh tiklqrnrke
     3601 gtmlvdgegp fkgvaagrkq gldlkallfv ggvpsdntvn kyaeitsgfv gcisrlvige
     3661 keidliddqt nsvgitncet caenpcnnag vcqeaatkng ytclcragys gkhcdyvgqs
     3721 cypgacgegt cvdketgfec ycphgktgsr cehsltiyep afhddksfva hetpkalrrt
     3781 rmnpsythnr lrarnaqvrk arsrphdhnk mhhtkh
//
LOCUS       XP_034185301            3815 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X14 [Osmia lignaria lignaria].
ACCESSION   XP_034185301
VERSION     XP_034185301.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329410.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3815
                     /product="terribly reduced optic lobes isoform X14"
                     /calculated_mol_wt=420766
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          344..378
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(349,357,368..369)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(361,364,368,374..375)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            371..375
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          383..417
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(388,396,407..408)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(400,403,407,413..414)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            410..414
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          419..453
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(424,432,443..444)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(436,439,443,449..450)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            446..450
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          459..493
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(464,472,483..484)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(476,479,483,489..490)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            486..490
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          498..532
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(503,511,522..523)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(515,518,522,528..529)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            525..529
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          534..568
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(539,547,558..559)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(551,554,558,564..565)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            561..565
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          573..607
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(578,586,597..598)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(590,593,597,603..604)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            600..604
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          610..643
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(616,625,636..637)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(629,632,636,642..643)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            639..643
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          674..707
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(678,686,697..698)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(690,693,697,703..704)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            700..704
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          710..744
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(715,723,734..735)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(727,730,734,740..741)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            737..741
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          748..782
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(753,761,772..773)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(765,768,772,778..779)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            775..779
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          784..818
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(789,797,808..809)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(801,804,808,814..815)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            811..815
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          832..866
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(837,845,856..857)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(849,852,856,862..863)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            859..863
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          870..901
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(875,883,894..895)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(887,890,894,900..901)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            897..901
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          917..952
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(922,931,942..943)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(935,938,942,948..949)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            945..949
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          977..1053
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          983..987
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          996..1000
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1016..1020
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1067..1101
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1072,1080,1091..1092)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1084,1087,1091,1097..1098)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1094..1098
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1106..1140
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1111,1119,1130..1131)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1123,1126,1130,1136..1137)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1133..1137
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1149..1184
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1154,1163,1174..1175)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1167,1170,1174,1180..1181)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1177..1181
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1202..1277
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1205..1209
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1218..1222
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1241..1245
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1255..1260
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1269..1272
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1379..1513
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1569..1622
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1570,1572,1585,1595,1597,1606)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1629..1675
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1630,1640,1647,1649,1658)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1746..1882
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1917..1965
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1917,1919,1926,1933,1936,1945)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1972..2018
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1972,1974,1984,1989,1991,2000)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2088..2224
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2420..2501
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2431..2435
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2446..2450
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2467..2471
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2515..2592
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2526..2530
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2558..2562
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2572..2577
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2585..2588
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2610..2689
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2621..2625
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2634..2638
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2654..2658
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2668..2673
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2681..2684
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2702..2780
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2713..2717
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2726..2730
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2745..2750
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2760..2765
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2773..2776
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2797..2873
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2804..2808
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2818..2822
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2839..2843
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2853..2858
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2869
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2888..2971
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2896..2900
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2909..2913
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2933..2941
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2951..2956
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2964..2967
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2995..3074
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3011..3015
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3025..3030
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3046..3050
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3060..3065
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3068..3071
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3086..3169
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3103..3107
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3116..3120
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3135..3139
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3149..3154
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3162..3165
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3184..3259
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3206..3210
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3225..3229
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3239..3244
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3252..3255
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3339..3412
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3345..3349
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3358..3362
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3378..3382
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3392..3397
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3405..3408
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3430..3499
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3433..3437
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3446..3450
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3466..3469
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3479..3484
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3492..3495
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3505..3657
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3680..3713
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3815
                     /gene="trol"
                     /coded_by="XM_034329410.2:322..11769"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfgcsagefa cdvsrciles
      361 hrcnfikecd dgsdehdcny pactstqfkc rngqcidssa hcngvedcrd rtdelncpcr
      421 edqfectpgf cvplnrrcdr fmdctngrde encsnmtrcr pdeftcsdgg cvsqnarcdg
      481 rsdcpdrsde hncnvttcsg dqfrcldgtc isidkrcnsn idcrngeden qcgcgeakfr
      541 ctdgqcigye lqcngveecs dgsderdcdl apcpsmdftc sdgsciskss vcdgfddcpr
      601 aedelhcere ctptqfkcvt gnkciegvyr cdghpdcpdh sdedcaneti thaiqptsqp
      661 wpgratdslr ecdpsremrc ddgkcvllrr kcdnifdcid gsdergcgvc tpaewkcasg
      721 ecipetercd nvvqcadgtd eagcvnecpa gmfrcndglc ldskrrcdgr phcldgsdei
      781 ncqcpdgelp cdngvcinkn ffcdrnadch dgsderdcpv idettdgdhv acrqdefacp
      841 hggcipqsav cngrndcpye edeincprgc gkdqfqcsng dciradqkcn gyidcvdgsd
      901 epeecerpgf hpmpgrcpag yimcvsdkdc vpqssmcngi pecrdrsdee nceqlkepsr
      961 lnlktypseq vikenpakqg revvfqcrde gplrarvrwl rgnnlplppg srdlngrlei
     1021 pniqldhggl yiceavgypa sttgqqvnvy ltvekfeppa tnkphvcmyd eatcsngdci
     1081 pksyvcngrl dctdgsdemr csphgcepne frcnntqcvs klwrcdgdkd cadgsdeenc
     1141 vpnrpgspcr vteytcasnd qcipksyhcd mekdcidgsd eigcspvyim kppppmitld
     1201 lgttlvivct aigvptpein wrlnwghipp kctmtsingt gtltcpdiqt edqgayscea
     1261 lntagfvfav pdaivmvnkp ddvcpkgmfn seartveeci scfcfgvate chsanlftyq
     1321 ipppfdrhki vavkqepdlr ilndisyqla vkplgrdglh ldestpflnq laaekdvpyf
     1381 alpenfhgsq lksyggylky rvryngtgvp nsapavvlsg nnyvlihkgk qlipnydnde
     1441 svrffegewl kkqgwsevpa tredimmtla nvdnilikve ydnspdldvq lthivmdtad
     1501 vrntglgsas fveecqcpsg ytglsceeca pgylrrktgl wlgqcyrdep vdcppgyygd
     1561 psrnipckac pcpltnpsnq fartchlgsd gqptcncppg yigrrceqcd vgyqgnplip
     1621 gdmcvarqqc dpegsissvv dpltgkcrck qyatgitcnq ckantfknqf gctscfcmgi
     1681 tnkcvssnwy rndvrvsftn sirdfslies rtphpppivd girlntvtre iiyndfpnrg
     1741 nndvyywqlp siflgdqvts yggnlkytvr yvpapggqss knnaadveli sandinllyf
     1801 srespepntp qtftvplleq ywqrndgtqa drehllmala dvsairikat ytthtdeaal
     1861 slvsldiaek yntgrarave veecscpigy kglscedcdv gytrasegly lgiceqcncn
     1921 ghsaqcnpdt gicencadht tgdfcevcep gyegdatrgt phdcvykdst pcrcneagsr
     1981 ssscigdrcd ckrnvegpqc nrcrpstfgl ssynpdgcte cycsgvtnqc hesslyvqqi
     2041 pvwvydnqhg ftltdstrqe iiddgfelnf amneigyryp dsrgrrlfws lpaiftgnkv
     2101 ksyggnltlt qhitaypgaq sykdqdiili gngitlfwtn pmdiqpdipl tysvplrese
     2161 wrrlttegpr vasridlmtv lsnleailvr athsermtat yisdisldta vehqtgnrra
     2221 vqvevcrcpt ghvgtscesc argyyrdpnd rsasylgscn pcpcnnnees ceitrsgqvk
     2281 chclpgytgq yclstgelmv sikpmkgevm answilftcs yefpeplyis fkltpfggmp
     2341 ltatgvtpgp imitekgaqr twyvyvqlep cnvechiqdr dgkelikvvt svtpetnvtt
     2401 ttpyppyipp prilvyikgp efqiveagtt vryhcagksl dngslhikwe keggqlptgr
     2461 tvddsqgllv irdvkvsdsg vyvcqvsdgv higyknvtlt vggsnpvapr avvmptslev
     2521 vegqpaefrc matghpqpqv ewirvhgpts pevvthngiw slraasknda aeykciarnn
     2581 vgvdeqtvil yvsdnpsmpp ttgilptitp sqwygtegda vlltcsqtsp elaslvwlrq
     2641 gnlpfsptat qrdgvltimn stqydsgvyi clattyvgth vnntaeitvv prrsppsikv
     2701 rpekqtvpqg svaevqcvts gepglqvkws kyhherlssn vqqvgdtlri vniqipdrgv
     2761 yvchvtgpsg shessaviev epreaprvei yprevppvil ggstdlqcra vagipmpelh
     2821 wshqngrpfp snikqlpggv lrlsnitand ggayvcsavn pvgsntavvy ievqsvpvim
     2881 isprsgilqv krgdrvklic rssgypqprv awvkhqnrid frtpfylsse aatpleavye
     2941 insaspddeg sytchannaa gmveervyir vednevyspd eypgpdkpen pddqkitvpk
     3001 dyfkipsggk vemrcyvrss dgnhiyldwk rtdhrplpeg stvhngilni pvadksaage
     3061 yvclgmdqar nvlfrakshl evlspprivl npprqtvrag enpsidcstt gdepmtiqwa
     3121 aigrnlpssv tqdrgvlqfh gitysdagky vctatndagt aeavaevlvn ehpfdessvv
     3181 haaqrdvvty agqpvrlrct vnvratihws rdgqplpsta rieddylelp rarpedsgry
     3241 icqiqtvhgv ssdyinlnvs lvnlhtecsr anrtqcsesk cicnvqgcfl ldylcnvyir
     3301 yyndigdnyy ntpqnrhylq qrratvtpav sveasqnpvn igdtvnirca csgthnpryh
     3361 wvrpnhpslp enaqeygnvl rltnvavsds gvyrciadtp egvfdhdfnl vvhdgnndap
     3421 aietkyapyg sslemdckpn lnpplkfhws klggllprda qifenklkli nvkaedagty
     3481 vctatnnlen ieiptvlvvt gvvpyfsqap esfialpplp dsylkfniei sfkpesydgi
     3541 ilyndesnrg ngdfillslv ggypvfsfdl gsgptiirae ksvtlsewht iklqrnrkeg
     3601 tmlvdgegpf kgvaagrkqg ldlkallfvg gvpsdntvnk yaeitsgfvg cisrlvigek
     3661 eidliddqtn svgitncetc aenpcnnagv cqeaatkngy tclcragysg khcdyvgqsc
     3721 ypgacgegtc vdketgfecy cphgktgsrc ehsltiyepa fhddksfvah etpkalrrtr
     3781 mnpsythnrl rarnaqvrka rsrphdhnkm hhtkh
//
LOCUS       XP_034185302            3806 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X15 [Osmia lignaria lignaria].
ACCESSION   XP_034185302
VERSION     XP_034185302.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329411.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3806
                     /product="terribly reduced optic lobes isoform X15"
                     /calculated_mol_wt=419557
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          800..834
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(805,813,824..825)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(817,820,824,830..831)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            827..831
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          838..872
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(843,851,862..863)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(855,858,862,868..869)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            865..869
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          874..908
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(879,887,898..899)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(891,894,898,904..905)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            901..905
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          922..956
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(927,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          960..991
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(965,973,984..985)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(977,980,984,990..991)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            987..991
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1007..1042
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1012,1021,1032..1033)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1025,1028,1032,1038..1039)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1035..1039
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1067..1143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1073..1077
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1086..1090
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1106..1110
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1157..1191
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1162,1170,1181..1182)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1174,1177,1181,1187..1188)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1184..1188
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1196..1230
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1201,1209,1220..1221)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1213,1216,1220,1226..1227)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1223..1227
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1239..1274
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1244,1253,1264..1265)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1257,1260,1264,1270..1271)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1267..1271
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1292..1367
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1295..1299
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1308..1312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1331..1335
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1345..1350
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1359..1362
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1469..1603
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1659..1712
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1660,1662,1675,1685,1687,1696)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1719..1765
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1720,1730,1737,1739,1748)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1836..1972
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2007..2055
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2007,2009,2016,2023,2026,2035)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2062..2108
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2062,2064,2074,2079,2081,2090)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2178..2314
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2411..2492
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2422..2426
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2437..2441
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2458..2462
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2506..2583
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2517..2521
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2530..2534
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2549..2553
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2563..2568
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2576..2579
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2601..2680
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2612..2616
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2625..2629
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2645..2649
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2659..2664
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2672..2675
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2693..2771
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2704..2708
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2717..2721
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2736..2741
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2751..2756
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2767
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2788..2864
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2795..2799
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2809..2813
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2830..2834
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2844..2849
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2857..2860
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2879..2962
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2887..2891
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2900..2904
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2924..2932
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2942..2947
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2955..2958
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2986..3065
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3002..3006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3016..3021
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3037..3041
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3051..3056
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3059..3062
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3077..3160
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3094..3098
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3107..3111
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3126..3130
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3140..3145
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3153..3156
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3175..3250
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3186..3190
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3197..3201
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3216..3220
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3235
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3243..3246
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3403
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3336..3340
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3349..3353
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3369..3373
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3383..3388
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3396..3399
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3421..3490
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3424..3428
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3437..3441
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3457..3460
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3470..3475
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3483..3486
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3496..3648
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3671..3704
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3806
                     /gene="trol"
                     /coded_by="XM_034329411.2:322..11742"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd eagcvnecpa
      841 gmfrcndglc ldskrrcdgr phcldgsdei ncqcpdgelp cdngvcinkn ffcdrnadch
      901 dgsderdcpv idettdgdhv acrqdefacp hggcipqsav cngrndcpye edeincprgc
      961 gkdqfqcsng dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag yimcvsdkdc
     1021 vpqssmcngi pecrdrsdee nceqlkepsr lnlktypseq vikenpakqg revvfqcrde
     1081 gplrarvrwl rgnnlplppg srdlngrlei pniqldhggl yiceavgypa sttgqqvnvy
     1141 ltvekfeppa tnkphvcmyd eatcsngdci pksyvcngrl dctdgsdemr csphgcepne
     1201 frcnntqcvs klwrcdgdkd cadgsdeenc vpnrpgspcr vteytcasnd qcipksyhcd
     1261 mekdcidgsd eigcspvyim kppppmitld lgttlvivct aigvptpein wrlnwghipp
     1321 kctmtsingt gtltcpdiqt edqgayscea lntagfvfav pdaivmvnkp ddvcpkgmfn
     1381 seartveeci scfcfgvate chsanlftyq ipppfdrhki vavkqepdlr ilndisyqla
     1441 vkplgrdglh ldestpflnq laaekdvpyf alpenfhgsq lksyggylky rvryngtgvp
     1501 nsapavvlsg nnyvlihkgk qlipnydnde svrffegewl kkqgwsevpa tredimmtla
     1561 nvdnilikve ydnspdldvq lthivmdtad vrntglgsas fveecqcpsg ytglsceeca
     1621 pgylrrktgl wlgqcyrdep vdcppgyygd psrnipckac pcpltnpsnq fartchlgsd
     1681 gqptcncppg yigrrceqcd vgyqgnplip gdmcvarqqc dpegsissvv dpltgkcrck
     1741 qyatgitcnq ckantfknqf gctscfcmgi tnkcvssnwy rndvrvsftn sirdfslies
     1801 rtphpppivd girlntvtre iiyndfpnrg nndvyywqlp siflgdqvts yggnlkytvr
     1861 yvpapggqss knnaadveli sandinllyf srespepntp qtftvplleq ywqrndgtqa
     1921 drehllmala dvsairikat ytthtdeaal slvsldiaek yntgrarave veecscpigy
     1981 kglscedcdv gytrasegly lgiceqcncn ghsaqcnpdt gicencadht tgdfcevcep
     2041 gyegdatrgt phdcvykdst pcrcneagsr ssscigdrcd ckrnvegpqc nrcrpstfgl
     2101 ssynpdgcte cycsgvtnqc hesslyvqqi pvwvydnqhg ftltdstrqe iiddgfelnf
     2161 amneigyryp dsrgrrlfws lpaiftgnkv ksyggnltlt qhitaypgaq sykdqdiili
     2221 gngitlfwtn pmdiqpdipl tysvplrese wrrlttegpr vasridlmtv lsnleailvr
     2281 athsermtat yisdisldta vehqtgnrra vqvevcrcpt ghvgtscesc argyyrdpnd
     2341 rsasylgscn pcpcnnnees ceitrsgqvk chclpgytgq yclstetnvt tttpyppyip
     2401 pprilvyikg pefqiveagt tvryhcagks ldngslhikw ekeggqlptg rtvddsqgll
     2461 virdvkvsds gvyvcqvsdg vhigyknvtl tvggsnpvap ravvmptsle vvegqpaefr
     2521 cmatghpqpq vewirvhgpt spevvthngi wslraasknd aaeykciarn nvgvdeqtvi
     2581 lyvsdnpsmp pttgilptit psqwygtegd avlltcsqts pelaslvwlr qgnlpfspta
     2641 tqrdgvltim nstqydsgvy iclattyvgt hvnntaeitv vprrsppsik vrpekqtvpq
     2701 gsvaevqcvt sgepglqvkw skyhherlss nvqqvgdtlr ivniqipdrg vyvchvtgps
     2761 gshessavie vepreaprve iyprevppvi lggstdlqcr avagipmpel hwshqngrpf
     2821 psnikqlpgg vlrlsnitan dggayvcsav npvgsntavv yievqsvpvi misprsgilq
     2881 vkrgdrvkli crssgypqpr vawvkhqnri dfrtpfylss eaatpleavy einsaspdde
     2941 gsytchanna agmveervyi rvednevysp deypgpdkpe npddqkitvp kdyfkipsgg
     3001 kvemrcyvrs sdgnhiyldw krtdhrplpe gstvhngiln ipvadksaag eyvclgmdqa
     3061 rnvlfraksh levlsppriv lnpprqtvra genpsidcst tgdepmtiqw aaigrnlpss
     3121 vtqdrgvlqf hgitysdagk yvctatndag taeavaevlv nehpfdessv vhaaqrdvvt
     3181 yagqpvrlrc tvnvratihw srdgqplpst arieddylel prarpedsgr yicqiqtvhg
     3241 vssdyinlnv slvnlhtecs ranrtqcses kcicnvqgcf lldylcnvyi ryyndigdny
     3301 yntpqnrhyl qqrratvtpa vsveasqnpv nigdtvnirc acsgthnpry hwvrpnhpsl
     3361 penaqeygnv lrltnvavsd sgvyrciadt pegvfdhdfn lvvhdgnnda paietkyapy
     3421 gsslemdckp nlnpplkfhw sklggllprd aqifenklkl invkaedagt yvctatnnle
     3481 nieiptvlvv tgvvpyfsqa pesfialppl pdsylkfnie isfkpesydg iilyndesnr
     3541 gngdfillsl vggypvfsfd lgsgptiira eksvtlsewh tiklqrnrke gtmlvdgegp
     3601 fkgvaagrkq gldlkallfv ggvpsdntvn kyaeitsgfv gcisrlvige keidliddqt
     3661 nsvgitncet caenpcnnag vcqeaatkng ytclcragys gkhcdyvgqs cypgacgegt
     3721 cvdketgfec ycphgktgsr cehsltiyep afhddksfva hetpkalrrt rmnpsythnr
     3781 lrarnaqvrk arsrphdhnk mhhtkh
//
LOCUS       XP_034185303            3780 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X16 [Osmia lignaria lignaria].
ACCESSION   XP_034185303
VERSION     XP_034185303.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329412.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3780
                     /product="terribly reduced optic lobes isoform X16"
                     /calculated_mol_wt=417018
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          639..672
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(643,651,662..663)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(655,658,662,668..669)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            665..669
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          675..709
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(680,688,699..700)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(692,695,699,705..706)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            702..706
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          713..747
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(718,726,737..738)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(730,733,737,743..744)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            740..744
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          749..783
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(754,762,773..774)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(766,769,773,779..780)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            776..780
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          797..831
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(802,810,821..822)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(814,817,821,827..828)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            824..828
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          835..866
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(840,848,859..860)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(852,855,859,865..866)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            862..866
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          882..917
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(887,896,907..908)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(900,903,907,913..914)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            910..914
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          942..1018
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          948..952
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          961..965
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          981..985
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1032..1066
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1037,1045,1056..1057)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1049,1052,1056,1062..1063)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1059..1063
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1071..1105
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1076,1084,1095..1096)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1088,1091,1095,1101..1102)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1098..1102
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1114..1149
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1119,1128,1139..1140)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1132,1135,1139,1145..1146)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1142..1146
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1167..1242
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1170..1174
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1183..1187
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1206..1210
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1220..1225
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1234..1237
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1344..1478
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1534..1587
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1535,1537,1550,1560,1562,1571)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1594..1640
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1595,1605,1612,1614,1623)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1711..1847
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1882..1930
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1882,1884,1891,1898,1901,1910)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1937..1983
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1937,1939,1949,1954,1956,1965)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2053..2189
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2385..2466
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2396..2400
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2411..2415
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2432..2436
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2480..2557
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2491..2495
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2504..2508
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2523..2527
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2537..2542
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2550..2553
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2575..2654
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2586..2590
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2599..2603
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2619..2623
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2633..2638
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2646..2649
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2667..2745
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2678..2682
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2691..2695
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2710..2715
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2730
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2738..2741
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2762..2838
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2769..2773
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2783..2787
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2804..2808
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2818..2823
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2831..2834
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2853..2936
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2861..2865
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2874..2878
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2898..2906
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2916..2921
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2929..2932
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2960..3039
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2976..2980
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3011..3015
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3025..3030
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3036
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3051..3134
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3068..3072
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3081..3085
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3100..3104
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3114..3119
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3127..3130
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3149..3224
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3160..3164
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3171..3175
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3190..3194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3204..3209
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3217..3220
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3377
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3310..3314
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3323..3327
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3343..3347
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3362
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3370..3373
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3395..3464
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3398..3402
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3411..3415
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3431..3434
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3444..3449
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3457..3460
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3470..3622
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3645..3678
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3780
                     /gene="trol"
                     /coded_by="XM_034329412.2:322..11664"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgqpwpgra tdslrecdps remrcddgkc vllrrkcdni
      661 fdcidgsder gcgvctpaew kcasgecipe tercdnvvqc adgtdeagcv necpagmfrc
      721 ndglcldskr rcdgrphcld gsdeincqcp dgelpcdngv cinknffcdr nadchdgsde
      781 rdcpvidett dgdhvacrqd efacphggci pqsavcngrn dcpyeedein cprgcgkdqf
      841 qcsngdcira dqkcngyidc vdgsdepeec erpgfhpmpg rcpagyimcv sdkdcvpqss
      901 mcngipecrd rsdeenceql kepsrlnlkt ypseqviken pakqgrevvf qcrdegplra
      961 rvrwlrgnnl plppgsrdln grleipniql dhgglyicea vgypasttgq qvnvyltvek
     1021 feppatnkph vcmydeatcs ngdcipksyv cngrldctdg sdemrcsphg cepnefrcnn
     1081 tqcvsklwrc dgdkdcadgs deencvpnrp gspcrvteyt casndqcipk syhcdmekdc
     1141 idgsdeigcs pvyimkpppp mitldlgttl vivctaigvp tpeinwrlnw ghippkctmt
     1201 singtgtltc pdiqtedqga yscealntag fvfavpdaiv mvnkpddvcp kgmfnseart
     1261 veeciscfcf gvatechsan lftyqipppf drhkivavkq epdlrilndi syqlavkplg
     1321 rdglhldest pflnqlaaek dvpyfalpen fhgsqlksyg gylkyrvryn gtgvpnsapa
     1381 vvlsgnnyvl ihkgkqlipn ydndesvrff egewlkkqgw sevpatredi mmtlanvdni
     1441 likveydnsp dldvqlthiv mdtadvrntg lgsasfveec qcpsgytgls ceecapgylr
     1501 rktglwlgqc yrdepvdcpp gyygdpsrni pckacpcplt npsnqfartc hlgsdgqptc
     1561 ncppgyigrr ceqcdvgyqg nplipgdmcv arqqcdpegs issvvdpltg kcrckqyatg
     1621 itcnqckant fknqfgctsc fcmgitnkcv ssnwyrndvr vsftnsirdf sliesrtphp
     1681 ppivdgirln tvtreiiynd fpnrgnndvy ywqlpsiflg dqvtsyggnl kytvryvpap
     1741 ggqssknnaa dvelisandi nllyfsresp epntpqtftv plleqywqrn dgtqadrehl
     1801 lmaladvsai rikatyttht deaalslvsl diaekyntgr araveveecs cpigykglsc
     1861 edcdvgytra seglylgice qcncnghsaq cnpdtgicen cadhttgdfc evcepgyegd
     1921 atrgtphdcv ykdstpcrcn eagsrsssci gdrcdckrnv egpqcnrcrp stfglssynp
     1981 dgctecycsg vtnqchessl yvqqipvwvy dnqhgftltd strqeiiddg felnfamnei
     2041 gyrypdsrgr rlfwslpaif tgnkvksygg nltltqhita ypgaqsykdq diiligngit
     2101 lfwtnpmdiq pdipltysvp lresewrrlt tegprvasri dlmtvlsnle ailvrathse
     2161 rmtatyisdi sldtavehqt gnrravqvev crcptghvgt scescargyy rdpndrsasy
     2221 lgscnpcpcn nneesceitr sgqvkchclp gytgqyclst gelmvsikpm kgevmanswi
     2281 lftcsyefpe plyisfkltp fggmpltatg vtpgpimite kgaqrtwyvy vqlepcnvec
     2341 hiqdrdgkel ikvvtsvtpe tnvttttpyp pyippprilv yikgpefqiv eagttvryhc
     2401 agksldngsl hikwekeggq lptgrtvdds qgllvirdvk vsdsgvyvcq vsdgvhigyk
     2461 nvtltvggsn pvapravvmp tslevvegqp aefrcmatgh pqpqvewirv hgptspevvt
     2521 hngiwslraa skndaaeykc iarnnvgvde qtvilyvsdn psmppttgil ptitpsqwyg
     2581 tegdavlltc sqtspelasl vwlrqgnlpf sptatqrdgv ltimnstqyd sgvyiclatt
     2641 yvgthvnnta eitvvprrsp psikvrpekq tvpqgsvaev qcvtsgepgl qvkwskyhhe
     2701 rlssnvqqvg dtlrivniqi pdrgvyvchv tgpsgshess avieveprea prveiyprev
     2761 ppvilggstd lqcravagip mpelhwshqn grpfpsnikq lpggvlrlsn itandggayv
     2821 csavnpvgsn tavvyievqs vpvimisprs gilqvkrgdr vklicrssgy pqprvawvkh
     2881 qnridfrtpf ylsseaatpl eavyeinsas pddegsytch annaagmvee rvyirvedne
     2941 vyspdeypgp dkpenpddqk itvpkdyfki psggkvemrc yvrssdgnhi yldwkrtdhr
     3001 plpegstvhn gilnipvadk saageyvclg mdqarnvlfr akshlevlsp privlnpprq
     3061 tvragenpsi dcsttgdepm tiqwaaigrn lpssvtqdrg vlqfhgitys dagkyvctat
     3121 ndagtaeava evlvnehpfd essvvhaaqr dvvtyagqpv rlrctvnvra tihwsrdgqp
     3181 lpstariedd ylelprarpe dsgryicqiq tvhgvssdyi nlnvslvnlh tecsranrtq
     3241 cseskcicnv qgcflldylc nvyiryyndi gdnyyntpqn rhylqqrrat vtpavsveas
     3301 qnpvnigdtv nircacsgth npryhwvrpn hpslpenaqe ygnvlrltnv avsdsgvyrc
     3361 iadtpegvfd hdfnlvvhdg nndapaietk yapygsslem dckpnlnppl kfhwsklggl
     3421 lprdaqifen klklinvkae dagtyvctat nnlenieipt vlvvtgvvpy fsqapesfia
     3481 lpplpdsylk fnieisfkpe sydgiilynd esnrgngdfi llslvggypv fsfdlgsgpt
     3541 iiraeksvtl sewhtiklqr nrkegtmlvd gegpfkgvaa grkqgldlka llfvggvpsd
     3601 ntvnkyaeit sgfvgcisrl vigekeidli ddqtnsvgit ncetcaenpc nnagvcqeaa
     3661 tkngytclcr agysgkhcdy vgqscypgac gegtcvdket gfecycphgk tgsrcehslt
     3721 iyepafhddk sfvahetpka lrrtrmnpsy thnrlrarna qvrkarsrph dhnkmhhtkh
//
LOCUS       XP_034185304            3780 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X17 [Osmia lignaria lignaria].
ACCESSION   XP_034185304
VERSION     XP_034185304.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329413.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3780
                     /product="terribly reduced optic lobes isoform X17"
                     /calculated_mol_wt=416894
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          749..783
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(754,762,773..774)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(766,769,773,779..780)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            776..780
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          797..831
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(802,810,821..822)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(814,817,821,827..828)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            824..828
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          835..866
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(840,848,859..860)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(852,855,859,865..866)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            862..866
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          882..917
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(887,896,907..908)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(900,903,907,913..914)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            910..914
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          942..1018
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          948..952
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          961..965
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          981..985
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1032..1066
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1037,1045,1056..1057)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1049,1052,1056,1062..1063)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1059..1063
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1071..1105
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1076,1084,1095..1096)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1088,1091,1095,1101..1102)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1098..1102
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1114..1149
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1119,1128,1139..1140)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1132,1135,1139,1145..1146)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1142..1146
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1167..1242
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1170..1174
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1183..1187
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1206..1210
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1220..1225
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1234..1237
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1344..1478
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1534..1587
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1535,1537,1550,1560,1562,1571)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1594..1640
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1595,1605,1612,1614,1623)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1711..1847
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1882..1930
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1882,1884,1891,1898,1901,1910)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1937..1983
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1937,1939,1949,1954,1956,1965)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2053..2189
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2385..2466
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2396..2400
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2411..2415
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2432..2436
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2480..2557
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2491..2495
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2504..2508
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2523..2527
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2537..2542
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2550..2553
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2575..2654
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2586..2590
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2599..2603
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2619..2623
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2633..2638
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2646..2649
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2667..2745
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2678..2682
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2691..2695
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2710..2715
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2730
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2738..2741
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2762..2838
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2769..2773
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2783..2787
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2804..2808
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2818..2823
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2831..2834
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2853..2936
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2861..2865
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2874..2878
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2898..2906
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2916..2921
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2929..2932
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2960..3039
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2976..2980
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3011..3015
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3025..3030
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3036
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3051..3134
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3068..3072
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3081..3085
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3100..3104
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3114..3119
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3127..3130
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3149..3224
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3160..3164
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3171..3175
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3190..3194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3204..3209
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3217..3220
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3377
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3310..3314
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3323..3327
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3343..3347
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3362
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3370..3373
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3395..3464
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3398..3402
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3411..3415
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3431..3434
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3444..3449
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3457..3460
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3470..3622
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3645..3678
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3780
                     /gene="trol"
                     /coded_by="XM_034329413.2:322..11664"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptkcp dgelpcdngv cinknffcdr nadchdgsde
      781 rdcpvidett dgdhvacrqd efacphggci pqsavcngrn dcpyeedein cprgcgkdqf
      841 qcsngdcira dqkcngyidc vdgsdepeec erpgfhpmpg rcpagyimcv sdkdcvpqss
      901 mcngipecrd rsdeenceql kepsrlnlkt ypseqviken pakqgrevvf qcrdegplra
      961 rvrwlrgnnl plppgsrdln grleipniql dhgglyicea vgypasttgq qvnvyltvek
     1021 feppatnkph vcmydeatcs ngdcipksyv cngrldctdg sdemrcsphg cepnefrcnn
     1081 tqcvsklwrc dgdkdcadgs deencvpnrp gspcrvteyt casndqcipk syhcdmekdc
     1141 idgsdeigcs pvyimkpppp mitldlgttl vivctaigvp tpeinwrlnw ghippkctmt
     1201 singtgtltc pdiqtedqga yscealntag fvfavpdaiv mvnkpddvcp kgmfnseart
     1261 veeciscfcf gvatechsan lftyqipppf drhkivavkq epdlrilndi syqlavkplg
     1321 rdglhldest pflnqlaaek dvpyfalpen fhgsqlksyg gylkyrvryn gtgvpnsapa
     1381 vvlsgnnyvl ihkgkqlipn ydndesvrff egewlkkqgw sevpatredi mmtlanvdni
     1441 likveydnsp dldvqlthiv mdtadvrntg lgsasfveec qcpsgytgls ceecapgylr
     1501 rktglwlgqc yrdepvdcpp gyygdpsrni pckacpcplt npsnqfartc hlgsdgqptc
     1561 ncppgyigrr ceqcdvgyqg nplipgdmcv arqqcdpegs issvvdpltg kcrckqyatg
     1621 itcnqckant fknqfgctsc fcmgitnkcv ssnwyrndvr vsftnsirdf sliesrtphp
     1681 ppivdgirln tvtreiiynd fpnrgnndvy ywqlpsiflg dqvtsyggnl kytvryvpap
     1741 ggqssknnaa dvelisandi nllyfsresp epntpqtftv plleqywqrn dgtqadrehl
     1801 lmaladvsai rikatyttht deaalslvsl diaekyntgr araveveecs cpigykglsc
     1861 edcdvgytra seglylgice qcncnghsaq cnpdtgicen cadhttgdfc evcepgyegd
     1921 atrgtphdcv ykdstpcrcn eagsrsssci gdrcdckrnv egpqcnrcrp stfglssynp
     1981 dgctecycsg vtnqchessl yvqqipvwvy dnqhgftltd strqeiiddg felnfamnei
     2041 gyrypdsrgr rlfwslpaif tgnkvksygg nltltqhita ypgaqsykdq diiligngit
     2101 lfwtnpmdiq pdipltysvp lresewrrlt tegprvasri dlmtvlsnle ailvrathse
     2161 rmtatyisdi sldtavehqt gnrravqvev crcptghvgt scescargyy rdpndrsasy
     2221 lgscnpcpcn nneesceitr sgqvkchclp gytgqyclst gelmvsikpm kgevmanswi
     2281 lftcsyefpe plyisfkltp fggmpltatg vtpgpimite kgaqrtwyvy vqlepcnvec
     2341 hiqdrdgkel ikvvtsvtpe tnvttttpyp pyippprilv yikgpefqiv eagttvryhc
     2401 agksldngsl hikwekeggq lptgrtvdds qgllvirdvk vsdsgvyvcq vsdgvhigyk
     2461 nvtltvggsn pvapravvmp tslevvegqp aefrcmatgh pqpqvewirv hgptspevvt
     2521 hngiwslraa skndaaeykc iarnnvgvde qtvilyvsdn psmppttgil ptitpsqwyg
     2581 tegdavlltc sqtspelasl vwlrqgnlpf sptatqrdgv ltimnstqyd sgvyiclatt
     2641 yvgthvnnta eitvvprrsp psikvrpekq tvpqgsvaev qcvtsgepgl qvkwskyhhe
     2701 rlssnvqqvg dtlrivniqi pdrgvyvchv tgpsgshess avieveprea prveiyprev
     2761 ppvilggstd lqcravagip mpelhwshqn grpfpsnikq lpggvlrlsn itandggayv
     2821 csavnpvgsn tavvyievqs vpvimisprs gilqvkrgdr vklicrssgy pqprvawvkh
     2881 qnridfrtpf ylsseaatpl eavyeinsas pddegsytch annaagmvee rvyirvedne
     2941 vyspdeypgp dkpenpddqk itvpkdyfki psggkvemrc yvrssdgnhi yldwkrtdhr
     3001 plpegstvhn gilnipvadk saageyvclg mdqarnvlfr akshlevlsp privlnpprq
     3061 tvragenpsi dcsttgdepm tiqwaaigrn lpssvtqdrg vlqfhgitys dagkyvctat
     3121 ndagtaeava evlvnehpfd essvvhaaqr dvvtyagqpv rlrctvnvra tihwsrdgqp
     3181 lpstariedd ylelprarpe dsgryicqiq tvhgvssdyi nlnvslvnlh tecsranrtq
     3241 cseskcicnv qgcflldylc nvyiryyndi gdnyyntpqn rhylqqrrat vtpavsveas
     3301 qnpvnigdtv nircacsgth npryhwvrpn hpslpenaqe ygnvlrltnv avsdsgvyrc
     3361 iadtpegvfd hdfnlvvhdg nndapaietk yapygsslem dckpnlnppl kfhwsklggl
     3421 lprdaqifen klklinvkae dagtyvctat nnlenieipt vlvvtgvvpy fsqapesfia
     3481 lpplpdsylk fnieisfkpe sydgiilynd esnrgngdfi llslvggypv fsfdlgsgpt
     3541 iiraeksvtl sewhtiklqr nrkegtmlvd gegpfkgvaa grkqgldlka llfvggvpsd
     3601 ntvnkyaeit sgfvgcisrl vigekeidli ddqtnsvgit ncetcaenpc nnagvcqeaa
     3661 tkngytclcr agysgkhcdy vgqscypgac gegtcvdket gfecycphgk tgsrcehslt
     3721 iyepafhddk sfvahetpka lrrtrmnpsy thnrlrarna qvrkarsrph dhnkmhhtkh
//
LOCUS       XP_034185306            3691 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X18 [Osmia lignaria lignaria].
ACCESSION   XP_034185306
VERSION     XP_034185306.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329415.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3691
                     /product="terribly reduced optic lobes isoform X18"
                     /calculated_mol_wt=407208
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          660..694
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(665,673,684..685)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(677,680,684,690..691)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            687..691
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          708..742
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(713,721,732..733)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(725,728,732,738..739)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            735..739
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          746..777
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(751,759,770..771)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(763,766,770,776..777)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            773..777
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          793..828
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(798,807,818..819)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(811,814,818,824..825)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            821..825
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          853..929
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          859..863
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          872..876
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          892..896
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          943..977
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(948,956,967..968)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(960,963,967,973..974)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            970..974
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          982..1016
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(987,995,1006..1007)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(999,1002,1006,1012..1013)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1009..1013
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1025..1060
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1030,1039,1050..1051)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1043,1046,1050,1056..1057)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1053..1057
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1078..1153
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1081..1085
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1094..1098
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1117..1121
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1131..1136
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1145..1148
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1255..1389
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1445..1498
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1446,1448,1461,1471,1473,1482)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1505..1551
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1506,1516,1523,1525,1534)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1622..1758
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1793..1841
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1793,1795,1802,1809,1812,1821)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1848..1894
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1848,1850,1860,1865,1867,1876)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1964..2100
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2296..2377
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2307..2311
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2322..2326
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2343..2347
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2391..2468
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2402..2406
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2415..2419
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2434..2438
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2448..2453
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2461..2464
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2486..2565
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2497..2501
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2510..2514
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2530..2534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2544..2549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2557..2560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2578..2656
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2589..2593
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2602..2606
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2621..2626
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2636..2641
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2649..2652
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2673..2749
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2680..2684
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2694..2698
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2715..2719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2729..2734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2742..2745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2847
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2772..2776
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2785..2789
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2809..2817
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2827..2832
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2843
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2871..2950
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2887..2891
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2901..2906
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..2926
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2936..2941
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2944..2947
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2962..3045
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2979..2983
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2992..2996
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3011..3015
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3025..3030
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3038..3041
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3060..3135
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3071..3075
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3082..3086
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3101..3105
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3115..3120
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3128..3131
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3215..3288
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3221..3225
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3234..3238
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3254..3258
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3268..3273
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3281..3284
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3306..3375
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3309..3313
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3322..3326
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3342..3345
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3355..3360
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3368..3371
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3381..3533
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3556..3589
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3691
                     /gene="trol"
                     /coded_by="XM_034329415.2:322..11397"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcec
      661 pdgelpcdng vcinknffcd rnadchdgsd erdcpvidet tdgdhvacrq defacphggc
      721 ipqsavcngr ndcpyeedei ncprgcgkdq fqcsngdcir adqkcngyid cvdgsdepee
      781 cerpgfhpmp grcpagyimc vsdkdcvpqs smcngipecr drsdeenceq lkepsrlnlk
      841 typseqvike npakqgrevv fqcrdegplr arvrwlrgnn lplppgsrdl ngrleipniq
      901 ldhgglyice avgypasttg qqvnvyltve kfeppatnkp hvcmydeatc sngdcipksy
      961 vcngrldctd gsdemrcsph gcepnefrcn ntqcvsklwr cdgdkdcadg sdeencvpnr
     1021 pgspcrvtey tcasndqcip ksyhcdmekd cidgsdeigc spvyimkppp pmitldlgtt
     1081 lvivctaigv ptpeinwrln wghippkctm tsingtgtlt cpdiqtedqg ayscealnta
     1141 gfvfavpdai vmvnkpddvc pkgmfnsear tveeciscfc fgvatechsa nlftyqippp
     1201 fdrhkivavk qepdlrilnd isyqlavkpl grdglhldes tpflnqlaae kdvpyfalpe
     1261 nfhgsqlksy ggylkyrvry ngtgvpnsap avvlsgnnyv lihkgkqlip nydndesvrf
     1321 fegewlkkqg wsevpatred immtlanvdn ilikveydns pdldvqlthi vmdtadvrnt
     1381 glgsasfvee cqcpsgytgl sceecapgyl rrktglwlgq cyrdepvdcp pgyygdpsrn
     1441 ipckacpcpl tnpsnqfart chlgsdgqpt cncppgyigr rceqcdvgyq gnplipgdmc
     1501 varqqcdpeg sissvvdplt gkcrckqyat gitcnqckan tfknqfgcts cfcmgitnkc
     1561 vssnwyrndv rvsftnsird fsliesrtph pppivdgirl ntvtreiiyn dfpnrgnndv
     1621 yywqlpsifl gdqvtsyggn lkytvryvpa pggqssknna advelisand inllyfsres
     1681 pepntpqtft vplleqywqr ndgtqadreh llmaladvsa irikatytth tdeaalslvs
     1741 ldiaekyntg raraveveec scpigykgls cedcdvgytr aseglylgic eqcncnghsa
     1801 qcnpdtgice ncadhttgdf cevcepgyeg datrgtphdc vykdstpcrc neagsrsssc
     1861 igdrcdckrn vegpqcnrcr pstfglssyn pdgctecycs gvtnqchess lyvqqipvwv
     1921 ydnqhgftlt dstrqeiidd gfelnfamne igyrypdsrg rrlfwslpai ftgnkvksyg
     1981 gnltltqhit aypgaqsykd qdiiligngi tlfwtnpmdi qpdipltysv plresewrrl
     2041 ttegprvasr idlmtvlsnl eailvraths ermtatyisd isldtavehq tgnrravqve
     2101 vcrcptghvg tscescargy yrdpndrsas ylgscnpcpc nnneesceit rsgqvkchcl
     2161 pgytgqycls tgelmvsikp mkgevmansw ilftcsyefp eplyisfklt pfggmpltat
     2221 gvtpgpimit ekgaqrtwyv yvqlepcnve chiqdrdgke likvvtsvtp etnvttttpy
     2281 ppyipppril vyikgpefqi veagttvryh cagksldngs lhikwekegg qlptgrtvdd
     2341 sqgllvirdv kvsdsgvyvc qvsdgvhigy knvtltvggs npvapravvm ptslevvegq
     2401 paefrcmatg hpqpqvewir vhgptspevv thngiwslra askndaaeyk ciarnnvgvd
     2461 eqtvilyvsd npsmppttgi lptitpsqwy gtegdavllt csqtspelas lvwlrqgnlp
     2521 fsptatqrdg vltimnstqy dsgvyiclat tyvgthvnnt aeitvvprrs ppsikvrpek
     2581 qtvpqgsvae vqcvtsgepg lqvkwskyhh erlssnvqqv gdtlrivniq ipdrgvyvch
     2641 vtgpsgshes savievepre aprveiypre vppvilggst dlqcravagi pmpelhwshq
     2701 ngrpfpsnik qlpggvlrls nitandggay vcsavnpvgs ntavvyievq svpvimispr
     2761 sgilqvkrgd rvklicrssg ypqprvawvk hqnridfrtp fylsseaatp leavyeinsa
     2821 spddegsytc hannaagmve ervyirvedn evyspdeypg pdkpenpddq kitvpkdyfk
     2881 ipsggkvemr cyvrssdgnh iyldwkrtdh rplpegstvh ngilnipvad ksaageyvcl
     2941 gmdqarnvlf rakshlevls pprivlnppr qtvragenps idcsttgdep mtiqwaaigr
     3001 nlpssvtqdr gvlqfhgity sdagkyvcta tndagtaeav aevlvnehpf dessvvhaaq
     3061 rdvvtyagqp vrlrctvnvr atihwsrdgq plpstaried dylelprarp edsgryicqi
     3121 qtvhgvssdy inlnvslvnl htecsranrt qcseskcicn vqgcflldyl cnvyiryynd
     3181 igdnyyntpq nrhylqqrra tvtpavsvea sqnpvnigdt vnircacsgt hnpryhwvrp
     3241 nhpslpenaq eygnvlrltn vavsdsgvyr ciadtpegvf dhdfnlvvhd gnndapaiet
     3301 kyapygssle mdckpnlnpp lkfhwsklgg llprdaqife nklklinvka edagtyvcta
     3361 tnnlenieip tvlvvtgvvp yfsqapesfi alpplpdsyl kfnieisfkp esydgiilyn
     3421 desnrgngdf illslvggyp vfsfdlgsgp tiiraeksvt lsewhtiklq rnrkegtmlv
     3481 dgegpfkgva agrkqgldlk allfvggvps dntvnkyaei tsgfvgcisr lvigekeidl
     3541 iddqtnsvgi tncetcaenp cnnagvcqea atkngytclc ragysgkhcd yvgqscypga
     3601 cgegtcvdke tgfecycphg ktgsrcehsl tiyepafhdd ksfvahetpk alrrtrmnps
     3661 ythnrlrarn aqvrkarsrp hdhnkmhhtk h
//
LOCUS       XP_034185307            3655 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X19 [Osmia lignaria lignaria].
ACCESSION   XP_034185307
VERSION     XP_034185307.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329416.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3655
                     /product="terribly reduced optic lobes isoform X19"
                     /calculated_mol_wt=403347
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          672..706
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(677,685,696..697)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(689,692,696,702..703)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            699..703
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          710..741
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(715,723,734..735)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(727,730,734,740..741)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            737..741
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          757..792
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(762,771,782..783)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(775,778,782,788..789)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            785..789
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          817..893
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          823..827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          836..840
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          856..860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          907..941
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(912,920,931..932)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(924,927,931,937..938)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            934..938
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          946..980
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(951,959,970..971)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(963,966,970,976..977)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            973..977
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          989..1024
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(994,1003,1014..1015)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1007,1010,1014,1020..1021)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1017..1021
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1042..1117
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1045..1049
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1058..1062
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1081..1085
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1095..1100
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1109..1112
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1219..1353
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1409..1462
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1410,1412,1425,1435,1437,1446)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1469..1515
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1470,1480,1487,1489,1498)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1586..1722
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1757..1805
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1757,1759,1766,1773,1776,1785)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1812..1858
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1812,1814,1824,1829,1831,1840)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1928..2064
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2260..2341
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2271..2275
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2286..2290
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2307..2311
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2355..2432
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2366..2370
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2379..2383
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2398..2402
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2412..2417
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2425..2428
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2450..2529
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2461..2465
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2474..2478
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2494..2498
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2508..2513
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2521..2524
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2542..2620
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2553..2557
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2570
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2585..2590
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2600..2605
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2616
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2637..2713
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2644..2648
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2658..2662
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2679..2683
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2693..2698
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2706..2709
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2728..2811
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2736..2740
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2749..2753
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2773..2781
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2791..2796
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2804..2807
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2835..2914
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2851..2855
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2865..2870
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2886..2890
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2900..2905
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2908..2911
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2926..3009
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2943..2947
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2956..2960
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2975..2979
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2989..2994
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3002..3005
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3024..3099
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3035..3039
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3046..3050
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3065..3069
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3079..3084
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3092..3095
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3179..3252
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3185..3189
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3198..3202
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3218..3222
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3232..3237
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3245..3248
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3270..3339
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3273..3277
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3286..3290
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3306..3309
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3319..3324
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3332..3335
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3345..3497
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3520..3553
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3655
                     /gene="trol"
                     /coded_by="XM_034329416.2:322..11289"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcecpdgelp cdngvcinkn ffcdrnadch dgsderdcpv
      661 idettdgdhv acrqdefacp hggcipqsav cngrndcpye edeincprgc gkdqfqcsng
      721 dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag yimcvsdkdc vpqssmcngi
      781 pecrdrsdee nceqlkepsr lnlktypseq vikenpakqg revvfqcrde gplrarvrwl
      841 rgnnlplppg srdlngrlei pniqldhggl yiceavgypa sttgqqvnvy ltvekfeppa
      901 tnkphvcmyd eatcsngdci pksyvcngrl dctdgsdemr csphgcepne frcnntqcvs
      961 klwrcdgdkd cadgsdeenc vpnrpgspcr vteytcasnd qcipksyhcd mekdcidgsd
     1021 eigcspvyim kppppmitld lgttlvivct aigvptpein wrlnwghipp kctmtsingt
     1081 gtltcpdiqt edqgayscea lntagfvfav pdaivmvnkp ddvcpkgmfn seartveeci
     1141 scfcfgvate chsanlftyq ipppfdrhki vavkqepdlr ilndisyqla vkplgrdglh
     1201 ldestpflnq laaekdvpyf alpenfhgsq lksyggylky rvryngtgvp nsapavvlsg
     1261 nnyvlihkgk qlipnydnde svrffegewl kkqgwsevpa tredimmtla nvdnilikve
     1321 ydnspdldvq lthivmdtad vrntglgsas fveecqcpsg ytglsceeca pgylrrktgl
     1381 wlgqcyrdep vdcppgyygd psrnipckac pcpltnpsnq fartchlgsd gqptcncppg
     1441 yigrrceqcd vgyqgnplip gdmcvarqqc dpegsissvv dpltgkcrck qyatgitcnq
     1501 ckantfknqf gctscfcmgi tnkcvssnwy rndvrvsftn sirdfslies rtphpppivd
     1561 girlntvtre iiyndfpnrg nndvyywqlp siflgdqvts yggnlkytvr yvpapggqss
     1621 knnaadveli sandinllyf srespepntp qtftvplleq ywqrndgtqa drehllmala
     1681 dvsairikat ytthtdeaal slvsldiaek yntgrarave veecscpigy kglscedcdv
     1741 gytrasegly lgiceqcncn ghsaqcnpdt gicencadht tgdfcevcep gyegdatrgt
     1801 phdcvykdst pcrcneagsr ssscigdrcd ckrnvegpqc nrcrpstfgl ssynpdgcte
     1861 cycsgvtnqc hesslyvqqi pvwvydnqhg ftltdstrqe iiddgfelnf amneigyryp
     1921 dsrgrrlfws lpaiftgnkv ksyggnltlt qhitaypgaq sykdqdiili gngitlfwtn
     1981 pmdiqpdipl tysvplrese wrrlttegpr vasridlmtv lsnleailvr athsermtat
     2041 yisdisldta vehqtgnrra vqvevcrcpt ghvgtscesc argyyrdpnd rsasylgscn
     2101 pcpcnnnees ceitrsgqvk chclpgytgq yclstgelmv sikpmkgevm answilftcs
     2161 yefpeplyis fkltpfggmp ltatgvtpgp imitekgaqr twyvyvqlep cnvechiqdr
     2221 dgkelikvvt svtpetnvtt ttpyppyipp prilvyikgp efqiveagtt vryhcagksl
     2281 dngslhikwe keggqlptgr tvddsqgllv irdvkvsdsg vyvcqvsdgv higyknvtlt
     2341 vggsnpvapr avvmptslev vegqpaefrc matghpqpqv ewirvhgpts pevvthngiw
     2401 slraasknda aeykciarnn vgvdeqtvil yvsdnpsmpp ttgilptitp sqwygtegda
     2461 vlltcsqtsp elaslvwlrq gnlpfsptat qrdgvltimn stqydsgvyi clattyvgth
     2521 vnntaeitvv prrsppsikv rpekqtvpqg svaevqcvts gepglqvkws kyhherlssn
     2581 vqqvgdtlri vniqipdrgv yvchvtgpsg shessaviev epreaprvei yprevppvil
     2641 ggstdlqcra vagipmpelh wshqngrpfp snikqlpggv lrlsnitand ggayvcsavn
     2701 pvgsntavvy ievqsvpvim isprsgilqv krgdrvklic rssgypqprv awvkhqnrid
     2761 frtpfylsse aatpleavye insaspddeg sytchannaa gmveervyir vednevyspd
     2821 eypgpdkpen pddqkitvpk dyfkipsggk vemrcyvrss dgnhiyldwk rtdhrplpeg
     2881 stvhngilni pvadksaage yvclgmdqar nvlfrakshl evlspprivl npprqtvrag
     2941 enpsidcstt gdepmtiqwa aigrnlpssv tqdrgvlqfh gitysdagky vctatndagt
     3001 aeavaevlvn ehpfdessvv haaqrdvvty agqpvrlrct vnvratihws rdgqplpsta
     3061 rieddylelp rarpedsgry icqiqtvhgv ssdyinlnvs lvnlhtecsr anrtqcsesk
     3121 cicnvqgcfl ldylcnvyir yyndigdnyy ntpqnrhylq qrratvtpav sveasqnpvn
     3181 igdtvnirca csgthnpryh wvrpnhpslp enaqeygnvl rltnvavsds gvyrciadtp
     3241 egvfdhdfnl vvhdgnndap aietkyapyg sslemdckpn lnpplkfhws klggllprda
     3301 qifenklkli nvkaedagty vctatnnlen ieiptvlvvt gvvpyfsqap esfialpplp
     3361 dsylkfniei sfkpesydgi ilyndesnrg ngdfillslv ggypvfsfdl gsgptiirae
     3421 ksvtlsewht iklqrnrkeg tmlvdgegpf kgvaagrkqg ldlkallfvg gvpsdntvnk
     3481 yaeitsgfvg cisrlvigek eidliddqtn svgitncetc aenpcnnagv cqeaatkngy
     3541 tclcragysg khcdyvgqsc ypgacgegtc vdketgfecy cphgktgsrc ehsltiyepa
     3601 fhddksfvah etpkalrrtr mnpsythnrl rarnaqvrka rsrphdhnkm hhtkh
//
LOCUS       XP_034185308            3470 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X20 [Osmia lignaria lignaria].
ACCESSION   XP_034185308
VERSION     XP_034185308.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329417.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3470
                     /product="terribly reduced optic lobes isoform X20"
                     /calculated_mol_wt=382839
     Region          38..72
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(43,51,62..63)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(55,58,62,68..69)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            65..69
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          74..108
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(79,87,98..99)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(91,94,98,104..105)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            101..105
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          114..148
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(119,127,138..139)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(131,134,138,144..145)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            141..145
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          153..187
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(158,166,177..178)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(170,173,177,183..184)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            180..184
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          189..223
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(194,202,213..214)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(206,209,213,219..220)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            216..220
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          228..262
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(233,241,252..253)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(245,248,252,258..259)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            255..259
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          265..298
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(271,280,291..292)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(284,287,291,297..298)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            294..298
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          329..362
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(333,341,352..353)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(345,348,352,358..359)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            355..359
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          365..399
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(370,378,389..390)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(382,385,389,395..396)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            392..396
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          403..437
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(408,416,427..428)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(420,423,427,433..434)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            430..434
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          439..473
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(444,452,463..464)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(456,459,463,469..470)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            466..470
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          487..521
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(492,500,511..512)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(504,507,511,517..518)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            514..518
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          525..556
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(530,538,549..550)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(542,545,549,555..556)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            552..556
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          572..607
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(577,586,597..598)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(590,593,597,603..604)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            600..604
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          632..708
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          638..642
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          651..655
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          671..675
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          722..756
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(727,735,746..747)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(739,742,746,752..753)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            749..753
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          761..795
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(766,774,785..786)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(778,781,785,791..792)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            788..792
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          804..839
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(809,818,829..830)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(822,825,829,835..836)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            832..836
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          857..932
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          860..864
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          873..877
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          896..900
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          910..915
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          924..927
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1034..1168
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1224..1277
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1225,1227,1240,1250,1252,1261)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1284..1330
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1285,1295,1302,1304,1313)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1401..1537
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1572..1620
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1572,1574,1581,1588,1591,1600)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1627..1673
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1627,1629,1639,1644,1646,1655)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1743..1879
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2075..2156
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2086..2090
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2101..2105
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2122..2126
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2170..2247
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2181..2185
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2194..2198
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2213..2217
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2227..2232
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2240..2243
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2265..2344
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2276..2280
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2289..2293
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2309..2313
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2323..2328
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2336..2339
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2357..2435
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2368..2372
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2381..2385
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2400..2405
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2415..2420
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2428..2431
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2528
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2459..2463
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2473..2477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2494..2498
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2508..2513
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2521..2524
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2543..2626
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2551..2555
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2564..2568
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2588..2596
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2606..2611
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2619..2622
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2650..2729
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2666..2670
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2680..2685
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2701..2705
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2715..2720
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2723..2726
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2741..2824
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2758..2762
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2771..2775
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2790..2794
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2804..2809
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2817..2820
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2839..2914
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2850..2854
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2861..2865
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2884
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2894..2899
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2907..2910
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2994..3067
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3000..3004
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3013..3017
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3037
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3047..3052
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3060..3063
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3085..3154
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3088..3092
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3101..3105
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3121..3124
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3134..3139
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3147..3150
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3160..3312
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3335..3368
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3470
                     /gene="trol"
                     /coded_by="XM_034329417.2:309..10721"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mghparrflv illlvlavtq sslgqrqrrp yrqrnracts tqfkcrngqc idssahcngv
       61 edcrdrtdel ncpcredqfe ctpgfcvpln rrcdrfmdct ngrdeencsn mtrcrpdeft
      121 csdggcvsqn arcdgrsdcp drsdehncnv ttcsgdqfrc ldgtcisidk rcnsnidcrn
      181 gedenqcgcg eakfrctdgq cigyelqcng veecsdgsde rdcdlapcps mdftcsdgsc
      241 iskssvcdgf ddcpraedel hcerectptq fkcvtgnkci egvyrcdghp dcpdhsdedc
      301 anetithaiq ptsqpwpgra tdslrecdps remrcddgkc vllrrkcdni fdcidgsder
      361 gcgvctpaew kcasgecipe tercdnvvqc adgtdeagcv necpagmfrc ndglcldskr
      421 rcdgrphcld gsdeincqcp dgelpcdngv cinknffcdr nadchdgsde rdcpvidett
      481 dgdhvacrqd efacphggci pqsavcngrn dcpyeedein cprgcgkdqf qcsngdcira
      541 dqkcngyidc vdgsdepeec erpgfhpmpg rcpagyimcv sdkdcvpqss mcngipecrd
      601 rsdeenceql kepsrlnlkt ypseqviken pakqgrevvf qcrdegplra rvrwlrgnnl
      661 plppgsrdln grleipniql dhgglyicea vgypasttgq qvnvyltvek feppatnkph
      721 vcmydeatcs ngdcipksyv cngrldctdg sdemrcsphg cepnefrcnn tqcvsklwrc
      781 dgdkdcadgs deencvpnrp gspcrvteyt casndqcipk syhcdmekdc idgsdeigcs
      841 pvyimkpppp mitldlgttl vivctaigvp tpeinwrlnw ghippkctmt singtgtltc
      901 pdiqtedqga yscealntag fvfavpdaiv mvnkpddvcp kgmfnseart veeciscfcf
      961 gvatechsan lftyqipppf drhkivavkq epdlrilndi syqlavkplg rdglhldest
     1021 pflnqlaaek dvpyfalpen fhgsqlksyg gylkyrvryn gtgvpnsapa vvlsgnnyvl
     1081 ihkgkqlipn ydndesvrff egewlkkqgw sevpatredi mmtlanvdni likveydnsp
     1141 dldvqlthiv mdtadvrntg lgsasfveec qcpsgytgls ceecapgylr rktglwlgqc
     1201 yrdepvdcpp gyygdpsrni pckacpcplt npsnqfartc hlgsdgqptc ncppgyigrr
     1261 ceqcdvgyqg nplipgdmcv arqqcdpegs issvvdpltg kcrckqyatg itcnqckant
     1321 fknqfgctsc fcmgitnkcv ssnwyrndvr vsftnsirdf sliesrtphp ppivdgirln
     1381 tvtreiiynd fpnrgnndvy ywqlpsiflg dqvtsyggnl kytvryvpap ggqssknnaa
     1441 dvelisandi nllyfsresp epntpqtftv plleqywqrn dgtqadrehl lmaladvsai
     1501 rikatyttht deaalslvsl diaekyntgr araveveecs cpigykglsc edcdvgytra
     1561 seglylgice qcncnghsaq cnpdtgicen cadhttgdfc evcepgyegd atrgtphdcv
     1621 ykdstpcrcn eagsrsssci gdrcdckrnv egpqcnrcrp stfglssynp dgctecycsg
     1681 vtnqchessl yvqqipvwvy dnqhgftltd strqeiiddg felnfamnei gyrypdsrgr
     1741 rlfwslpaif tgnkvksygg nltltqhita ypgaqsykdq diiligngit lfwtnpmdiq
     1801 pdipltysvp lresewrrlt tegprvasri dlmtvlsnle ailvrathse rmtatyisdi
     1861 sldtavehqt gnrravqvev crcptghvgt scescargyy rdpndrsasy lgscnpcpcn
     1921 nneesceitr sgqvkchclp gytgqyclst gelmvsikpm kgevmanswi lftcsyefpe
     1981 plyisfkltp fggmpltatg vtpgpimite kgaqrtwyvy vqlepcnvec hiqdrdgkel
     2041 ikvvtsvtpe tnvttttpyp pyippprilv yikgpefqiv eagttvryhc agksldngsl
     2101 hikwekeggq lptgrtvdds qgllvirdvk vsdsgvyvcq vsdgvhigyk nvtltvggsn
     2161 pvapravvmp tslevvegqp aefrcmatgh pqpqvewirv hgptspevvt hngiwslraa
     2221 skndaaeykc iarnnvgvde qtvilyvsdn psmppttgil ptitpsqwyg tegdavlltc
     2281 sqtspelasl vwlrqgnlpf sptatqrdgv ltimnstqyd sgvyiclatt yvgthvnnta
     2341 eitvvprrsp psikvrpekq tvpqgsvaev qcvtsgepgl qvkwskyhhe rlssnvqqvg
     2401 dtlrivniqi pdrgvyvchv tgpsgshess avieveprea prveiyprev ppvilggstd
     2461 lqcravagip mpelhwshqn grpfpsnikq lpggvlrlsn itandggayv csavnpvgsn
     2521 tavvyievqs vpvimisprs gilqvkrgdr vklicrssgy pqprvawvkh qnridfrtpf
     2581 ylsseaatpl eavyeinsas pddegsytch annaagmvee rvyirvedne vyspdeypgp
     2641 dkpenpddqk itvpkdyfki psggkvemrc yvrssdgnhi yldwkrtdhr plpegstvhn
     2701 gilnipvadk saageyvclg mdqarnvlfr akshlevlsp privlnpprq tvragenpsi
     2761 dcsttgdepm tiqwaaigrn lpssvtqdrg vlqfhgitys dagkyvctat ndagtaeava
     2821 evlvnehpfd essvvhaaqr dvvtyagqpv rlrctvnvra tihwsrdgqp lpstariedd
     2881 ylelprarpe dsgryicqiq tvhgvssdyi nlnvslvnlh tecsranrtq cseskcicnv
     2941 qgcflldylc nvyiryyndi gdnyyntpqn rhylqqrrat vtpavsveas qnpvnigdtv
     3001 nircacsgth npryhwvrpn hpslpenaqe ygnvlrltnv avsdsgvyrc iadtpegvfd
     3061 hdfnlvvhdg nndapaietk yapygsslem dckpnlnppl kfhwsklggl lprdaqifen
     3121 klklinvkae dagtyvctat nnlenieipt vlvvtgvvpy fsqapesfia lpplpdsylk
     3181 fnieisfkpe sydgiilynd esnrgngdfi llslvggypv fsfdlgsgpt iiraeksvtl
     3241 sewhtiklqr nrkegtmlvd gegpfkgvaa grkqgldlka llfvggvpsd ntvnkyaeit
     3301 sgfvgcisrl vigekeidli ddqtnsvgit ncetcaenpc nnagvcqeaa tkngytclcr
     3361 agysgkhcdy vgqscypgac gegtcvdket gfecycphgk tgsrcehslt iyepafhddk
     3421 sfvahetpka lrrtrmnpsy thnrlrarna qvrkarsrph dhnkmhhtkh
//
LOCUS       XP_034185309            3470 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X20 [Osmia lignaria lignaria].
ACCESSION   XP_034185309
VERSION     XP_034185309.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329418.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3470
                     /product="terribly reduced optic lobes isoform X20"
                     /calculated_mol_wt=382839
     Region          38..72
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(43,51,62..63)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(55,58,62,68..69)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            65..69
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          74..108
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(79,87,98..99)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(91,94,98,104..105)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            101..105
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          114..148
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(119,127,138..139)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(131,134,138,144..145)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            141..145
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          153..187
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(158,166,177..178)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(170,173,177,183..184)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            180..184
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          189..223
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(194,202,213..214)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(206,209,213,219..220)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            216..220
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          228..262
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(233,241,252..253)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(245,248,252,258..259)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            255..259
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          265..298
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(271,280,291..292)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(284,287,291,297..298)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            294..298
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          329..362
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(333,341,352..353)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(345,348,352,358..359)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            355..359
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          365..399
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(370,378,389..390)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(382,385,389,395..396)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            392..396
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          403..437
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(408,416,427..428)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(420,423,427,433..434)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            430..434
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          439..473
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(444,452,463..464)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(456,459,463,469..470)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            466..470
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          487..521
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(492,500,511..512)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(504,507,511,517..518)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            514..518
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          525..556
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(530,538,549..550)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(542,545,549,555..556)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            552..556
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          572..607
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(577,586,597..598)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(590,593,597,603..604)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            600..604
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          632..708
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          638..642
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          651..655
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          671..675
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          722..756
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(727,735,746..747)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(739,742,746,752..753)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            749..753
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          761..795
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(766,774,785..786)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(778,781,785,791..792)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            788..792
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          804..839
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(809,818,829..830)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(822,825,829,835..836)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            832..836
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          857..932
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          860..864
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          873..877
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          896..900
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          910..915
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          924..927
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1034..1168
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1224..1277
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1225,1227,1240,1250,1252,1261)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1284..1330
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1285,1295,1302,1304,1313)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1401..1537
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1572..1620
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1572,1574,1581,1588,1591,1600)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1627..1673
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1627,1629,1639,1644,1646,1655)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1743..1879
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2075..2156
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2086..2090
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2101..2105
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2122..2126
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2170..2247
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2181..2185
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2194..2198
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2213..2217
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2227..2232
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2240..2243
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2265..2344
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2276..2280
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2289..2293
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2309..2313
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2323..2328
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2336..2339
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2357..2435
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2368..2372
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2381..2385
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2400..2405
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2415..2420
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2428..2431
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2528
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2459..2463
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2473..2477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2494..2498
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2508..2513
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2521..2524
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2543..2626
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2551..2555
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2564..2568
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2588..2596
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2606..2611
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2619..2622
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2650..2729
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2666..2670
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2680..2685
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2701..2705
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2715..2720
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2723..2726
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2741..2824
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2758..2762
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2771..2775
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2790..2794
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2804..2809
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2817..2820
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2839..2914
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2850..2854
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2861..2865
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2884
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2894..2899
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2907..2910
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2994..3067
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3000..3004
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3013..3017
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3037
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3047..3052
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3060..3063
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3085..3154
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3088..3092
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3101..3105
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3121..3124
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3134..3139
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3147..3150
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3160..3312
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3335..3368
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3470
                     /gene="trol"
                     /coded_by="XM_034329418.2:305..10717"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mghparrflv illlvlavtq sslgqrqrrp yrqrnracts tqfkcrngqc idssahcngv
       61 edcrdrtdel ncpcredqfe ctpgfcvpln rrcdrfmdct ngrdeencsn mtrcrpdeft
      121 csdggcvsqn arcdgrsdcp drsdehncnv ttcsgdqfrc ldgtcisidk rcnsnidcrn
      181 gedenqcgcg eakfrctdgq cigyelqcng veecsdgsde rdcdlapcps mdftcsdgsc
      241 iskssvcdgf ddcpraedel hcerectptq fkcvtgnkci egvyrcdghp dcpdhsdedc
      301 anetithaiq ptsqpwpgra tdslrecdps remrcddgkc vllrrkcdni fdcidgsder
      361 gcgvctpaew kcasgecipe tercdnvvqc adgtdeagcv necpagmfrc ndglcldskr
      421 rcdgrphcld gsdeincqcp dgelpcdngv cinknffcdr nadchdgsde rdcpvidett
      481 dgdhvacrqd efacphggci pqsavcngrn dcpyeedein cprgcgkdqf qcsngdcira
      541 dqkcngyidc vdgsdepeec erpgfhpmpg rcpagyimcv sdkdcvpqss mcngipecrd
      601 rsdeenceql kepsrlnlkt ypseqviken pakqgrevvf qcrdegplra rvrwlrgnnl
      661 plppgsrdln grleipniql dhgglyicea vgypasttgq qvnvyltvek feppatnkph
      721 vcmydeatcs ngdcipksyv cngrldctdg sdemrcsphg cepnefrcnn tqcvsklwrc
      781 dgdkdcadgs deencvpnrp gspcrvteyt casndqcipk syhcdmekdc idgsdeigcs
      841 pvyimkpppp mitldlgttl vivctaigvp tpeinwrlnw ghippkctmt singtgtltc
      901 pdiqtedqga yscealntag fvfavpdaiv mvnkpddvcp kgmfnseart veeciscfcf
      961 gvatechsan lftyqipppf drhkivavkq epdlrilndi syqlavkplg rdglhldest
     1021 pflnqlaaek dvpyfalpen fhgsqlksyg gylkyrvryn gtgvpnsapa vvlsgnnyvl
     1081 ihkgkqlipn ydndesvrff egewlkkqgw sevpatredi mmtlanvdni likveydnsp
     1141 dldvqlthiv mdtadvrntg lgsasfveec qcpsgytgls ceecapgylr rktglwlgqc
     1201 yrdepvdcpp gyygdpsrni pckacpcplt npsnqfartc hlgsdgqptc ncppgyigrr
     1261 ceqcdvgyqg nplipgdmcv arqqcdpegs issvvdpltg kcrckqyatg itcnqckant
     1321 fknqfgctsc fcmgitnkcv ssnwyrndvr vsftnsirdf sliesrtphp ppivdgirln
     1381 tvtreiiynd fpnrgnndvy ywqlpsiflg dqvtsyggnl kytvryvpap ggqssknnaa
     1441 dvelisandi nllyfsresp epntpqtftv plleqywqrn dgtqadrehl lmaladvsai
     1501 rikatyttht deaalslvsl diaekyntgr araveveecs cpigykglsc edcdvgytra
     1561 seglylgice qcncnghsaq cnpdtgicen cadhttgdfc evcepgyegd atrgtphdcv
     1621 ykdstpcrcn eagsrsssci gdrcdckrnv egpqcnrcrp stfglssynp dgctecycsg
     1681 vtnqchessl yvqqipvwvy dnqhgftltd strqeiiddg felnfamnei gyrypdsrgr
     1741 rlfwslpaif tgnkvksygg nltltqhita ypgaqsykdq diiligngit lfwtnpmdiq
     1801 pdipltysvp lresewrrlt tegprvasri dlmtvlsnle ailvrathse rmtatyisdi
     1861 sldtavehqt gnrravqvev crcptghvgt scescargyy rdpndrsasy lgscnpcpcn
     1921 nneesceitr sgqvkchclp gytgqyclst gelmvsikpm kgevmanswi lftcsyefpe
     1981 plyisfkltp fggmpltatg vtpgpimite kgaqrtwyvy vqlepcnvec hiqdrdgkel
     2041 ikvvtsvtpe tnvttttpyp pyippprilv yikgpefqiv eagttvryhc agksldngsl
     2101 hikwekeggq lptgrtvdds qgllvirdvk vsdsgvyvcq vsdgvhigyk nvtltvggsn
     2161 pvapravvmp tslevvegqp aefrcmatgh pqpqvewirv hgptspevvt hngiwslraa
     2221 skndaaeykc iarnnvgvde qtvilyvsdn psmppttgil ptitpsqwyg tegdavlltc
     2281 sqtspelasl vwlrqgnlpf sptatqrdgv ltimnstqyd sgvyiclatt yvgthvnnta
     2341 eitvvprrsp psikvrpekq tvpqgsvaev qcvtsgepgl qvkwskyhhe rlssnvqqvg
     2401 dtlrivniqi pdrgvyvchv tgpsgshess avieveprea prveiyprev ppvilggstd
     2461 lqcravagip mpelhwshqn grpfpsnikq lpggvlrlsn itandggayv csavnpvgsn
     2521 tavvyievqs vpvimisprs gilqvkrgdr vklicrssgy pqprvawvkh qnridfrtpf
     2581 ylsseaatpl eavyeinsas pddegsytch annaagmvee rvyirvedne vyspdeypgp
     2641 dkpenpddqk itvpkdyfki psggkvemrc yvrssdgnhi yldwkrtdhr plpegstvhn
     2701 gilnipvadk saageyvclg mdqarnvlfr akshlevlsp privlnpprq tvragenpsi
     2761 dcsttgdepm tiqwaaigrn lpssvtqdrg vlqfhgitys dagkyvctat ndagtaeava
     2821 evlvnehpfd essvvhaaqr dvvtyagqpv rlrctvnvra tihwsrdgqp lpstariedd
     2881 ylelprarpe dsgryicqiq tvhgvssdyi nlnvslvnlh tecsranrtq cseskcicnv
     2941 qgcflldylc nvyiryyndi gdnyyntpqn rhylqqrrat vtpavsveas qnpvnigdtv
     3001 nircacsgth npryhwvrpn hpslpenaqe ygnvlrltnv avsdsgvyrc iadtpegvfd
     3061 hdfnlvvhdg nndapaietk yapygsslem dckpnlnppl kfhwsklggl lprdaqifen
     3121 klklinvkae dagtyvctat nnlenieipt vlvvtgvvpy fsqapesfia lpplpdsylk
     3181 fnieisfkpe sydgiilynd esnrgngdfi llslvggypv fsfdlgsgpt iiraeksvtl
     3241 sewhtiklqr nrkegtmlvd gegpfkgvaa grkqgldlka llfvggvpsd ntvnkyaeit
     3301 sgfvgcisrl vigekeidli ddqtnsvgit ncetcaenpc nnagvcqeaa tkngytclcr
     3361 agysgkhcdy vgqscypgac gegtcvdket gfecycphgk tgsrcehslt iyepafhddk
     3421 sfvahetpka lrrtrmnpsy thnrlrarna qvrkarsrph dhnkmhhtkh
//
LOCUS       XP_034185310            3431 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X21 [Osmia lignaria lignaria].
ACCESSION   XP_034185310
VERSION     XP_034185310.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329419.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3431
                     /product="terribly reduced optic lobes isoform X21"
                     /calculated_mol_wt=378263
     Region          38..72
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(43,51,62..63)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(55,58,62,68..69)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            65..69
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          75..109
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(80,88,99..100)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(92,95,99,105..106)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            102..106
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          114..148
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(119,127,138..139)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(131,134,138,144..145)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            141..145
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          150..184
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(155,163,174..175)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(167,170,174,180..181)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            177..181
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          189..223
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(194,202,213..214)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(206,209,213,219..220)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            216..220
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          226..259
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(232,241,252..253)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(245,248,252,258..259)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            255..259
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          290..323
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(294,302,313..314)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(306,309,313,319..320)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            316..320
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          326..360
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(331,339,350..351)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(343,346,350,356..357)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            353..357
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          364..398
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(369,377,388..389)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(381,384,388,394..395)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            391..395
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          400..434
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(405,413,424..425)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(417,420,424,430..431)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            427..431
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          448..482
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(453,461,472..473)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(465,468,472,478..479)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            475..479
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          486..517
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(491,499,510..511)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(503,506,510,516..517)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            513..517
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          533..568
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(538,547,558..559)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(551,554,558,564..565)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            561..565
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          593..669
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          599..603
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          612..616
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          632..636
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          683..717
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(688,696,707..708)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(700,703,707,713..714)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            710..714
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          722..756
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(727,735,746..747)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(739,742,746,752..753)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            749..753
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          765..800
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(770,779,790..791)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(783,786,790,796..797)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            793..797
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          818..893
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          821..825
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          834..838
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          857..861
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          871..876
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          885..888
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          995..1129
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1185..1238
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1186,1188,1201,1211,1213,1222)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1245..1291
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1246,1256,1263,1265,1274)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1362..1498
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1533..1581
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1533,1535,1542,1549,1552,1561)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1588..1634
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1588,1590,1600,1605,1607,1616)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1704..1840
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2036..2117
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2047..2051
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2062..2066
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2083..2087
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2131..2208
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2142..2146
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2155..2159
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2174..2178
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2188..2193
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2201..2204
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2226..2305
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2237..2241
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2250..2254
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2270..2274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2284..2289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2297..2300
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2318..2396
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2329..2333
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2342..2346
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2361..2366
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2376..2381
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2389..2392
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2489
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2420..2424
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2434..2438
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2455..2459
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2469..2474
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2482..2485
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2504..2587
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2512..2516
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2525..2529
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2549..2557
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2567..2572
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2580..2583
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2611..2690
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2627..2631
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2641..2646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2662..2666
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2676..2681
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2684..2687
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2702..2785
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2719..2723
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2732..2736
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2751..2755
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2765..2770
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2778..2781
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2800..2875
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2811..2815
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2822..2826
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2841..2845
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2855..2860
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2868..2871
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2955..3028
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2961..2965
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2974..2978
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2994..2998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3008..3013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3021..3024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3046..3115
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3049..3053
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3062..3066
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3082..3085
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3095..3100
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3108..3111
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3121..3273
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3296..3329
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3431
                     /gene="trol"
                     /coded_by="XM_034329419.2:308..10603"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mghparrflv illlvlavtq sslgqrqrrp yrqrnracts tqfkcrngqc idssahcngv
       61 edcrdrtdel ncprcrpdef tcsdggcvsq narcdgrsdc pdrsdehncn vttcsgdqfr
      121 cldgtcisid krcnsnidcr ngedenqcgc geakfrctdg qcigyelqcn gveecsdgsd
      181 erdcdlapcp smdftcsdgs ciskssvcdg fddcpraede lhcerectpt qfkcvtgnkc
      241 iegvyrcdgh pdcpdhsded canetithai qptsqpwpgr atdslrecdp sremrcddgk
      301 cvllrrkcdn ifdcidgsde rgcgvctpae wkcasgecip etercdnvvq cadgtdeagc
      361 vnecpagmfr cndglcldsk rrcdgrphcl dgsdeincqc pdgelpcdng vcinknffcd
      421 rnadchdgsd erdcpvidet tdgdhvacrq defacphggc ipqsavcngr ndcpyeedei
      481 ncprgcgkdq fqcsngdcir adqkcngyid cvdgsdepee cerpgfhpmp grcpagyimc
      541 vsdkdcvpqs smcngipecr drsdeenceq lkepsrlnlk typseqvike npakqgrevv
      601 fqcrdegplr arvrwlrgnn lplppgsrdl ngrleipniq ldhgglyice avgypasttg
      661 qqvnvyltve kfeppatnkp hvcmydeatc sngdcipksy vcngrldctd gsdemrcsph
      721 gcepnefrcn ntqcvsklwr cdgdkdcadg sdeencvpnr pgspcrvtey tcasndqcip
      781 ksyhcdmekd cidgsdeigc spvyimkppp pmitldlgtt lvivctaigv ptpeinwrln
      841 wghippkctm tsingtgtlt cpdiqtedqg ayscealnta gfvfavpdai vmvnkpddvc
      901 pkgmfnsear tveeciscfc fgvatechsa nlftyqippp fdrhkivavk qepdlrilnd
      961 isyqlavkpl grdglhldes tpflnqlaae kdvpyfalpe nfhgsqlksy ggylkyrvry
     1021 ngtgvpnsap avvlsgnnyv lihkgkqlip nydndesvrf fegewlkkqg wsevpatred
     1081 immtlanvdn ilikveydns pdldvqlthi vmdtadvrnt glgsasfvee cqcpsgytgl
     1141 sceecapgyl rrktglwlgq cyrdepvdcp pgyygdpsrn ipckacpcpl tnpsnqfart
     1201 chlgsdgqpt cncppgyigr rceqcdvgyq gnplipgdmc varqqcdpeg sissvvdplt
     1261 gkcrckqyat gitcnqckan tfknqfgcts cfcmgitnkc vssnwyrndv rvsftnsird
     1321 fsliesrtph pppivdgirl ntvtreiiyn dfpnrgnndv yywqlpsifl gdqvtsyggn
     1381 lkytvryvpa pggqssknna advelisand inllyfsres pepntpqtft vplleqywqr
     1441 ndgtqadreh llmaladvsa irikatytth tdeaalslvs ldiaekyntg raraveveec
     1501 scpigykgls cedcdvgytr aseglylgic eqcncnghsa qcnpdtgice ncadhttgdf
     1561 cevcepgyeg datrgtphdc vykdstpcrc neagsrsssc igdrcdckrn vegpqcnrcr
     1621 pstfglssyn pdgctecycs gvtnqchess lyvqqipvwv ydnqhgftlt dstrqeiidd
     1681 gfelnfamne igyrypdsrg rrlfwslpai ftgnkvksyg gnltltqhit aypgaqsykd
     1741 qdiiligngi tlfwtnpmdi qpdipltysv plresewrrl ttegprvasr idlmtvlsnl
     1801 eailvraths ermtatyisd isldtavehq tgnrravqve vcrcptghvg tscescargy
     1861 yrdpndrsas ylgscnpcpc nnneesceit rsgqvkchcl pgytgqycls tgelmvsikp
     1921 mkgevmansw ilftcsyefp eplyisfklt pfggmpltat gvtpgpimit ekgaqrtwyv
     1981 yvqlepcnve chiqdrdgke likvvtsvtp etnvttttpy ppyipppril vyikgpefqi
     2041 veagttvryh cagksldngs lhikwekegg qlptgrtvdd sqgllvirdv kvsdsgvyvc
     2101 qvsdgvhigy knvtltvggs npvapravvm ptslevvegq paefrcmatg hpqpqvewir
     2161 vhgptspevv thngiwslra askndaaeyk ciarnnvgvd eqtvilyvsd npsmppttgi
     2221 lptitpsqwy gtegdavllt csqtspelas lvwlrqgnlp fsptatqrdg vltimnstqy
     2281 dsgvyiclat tyvgthvnnt aeitvvprrs ppsikvrpek qtvpqgsvae vqcvtsgepg
     2341 lqvkwskyhh erlssnvqqv gdtlrivniq ipdrgvyvch vtgpsgshes savievepre
     2401 aprveiypre vppvilggst dlqcravagi pmpelhwshq ngrpfpsnik qlpggvlrls
     2461 nitandggay vcsavnpvgs ntavvyievq svpvimispr sgilqvkrgd rvklicrssg
     2521 ypqprvawvk hqnridfrtp fylsseaatp leavyeinsa spddegsytc hannaagmve
     2581 ervyirvedn evyspdeypg pdkpenpddq kitvpkdyfk ipsggkvemr cyvrssdgnh
     2641 iyldwkrtdh rplpegstvh ngilnipvad ksaageyvcl gmdqarnvlf rakshlevls
     2701 pprivlnppr qtvragenps idcsttgdep mtiqwaaigr nlpssvtqdr gvlqfhgity
     2761 sdagkyvcta tndagtaeav aevlvnehpf dessvvhaaq rdvvtyagqp vrlrctvnvr
     2821 atihwsrdgq plpstaried dylelprarp edsgryicqi qtvhgvssdy inlnvslvnl
     2881 htecsranrt qcseskcicn vqgcflldyl cnvyiryynd igdnyyntpq nrhylqqrra
     2941 tvtpavsvea sqnpvnigdt vnircacsgt hnpryhwvrp nhpslpenaq eygnvlrltn
     3001 vavsdsgvyr ciadtpegvf dhdfnlvvhd gnndapaiet kyapygssle mdckpnlnpp
     3061 lkfhwsklgg llprdaqife nklklinvka edagtyvcta tnnlenieip tvlvvtgvvp
     3121 yfsqapesfi alpplpdsyl kfnieisfkp esydgiilyn desnrgngdf illslvggyp
     3181 vfsfdlgsgp tiiraeksvt lsewhtiklq rnrkegtmlv dgegpfkgva agrkqgldlk
     3241 allfvggvps dntvnkyaei tsgfvgcisr lvigekeidl iddqtnsvgi tncetcaenp
     3301 cnnagvcqea atkngytclc ragysgkhcd yvgqscypga cgegtcvdke tgfecycphg
     3361 ktgsrcehsl tiyepafhdd ksfvahetpk alrrtrmnps ythnrlrarn aqvrkarsrp
     3421 hdhnkmhhtk h
//
LOCUS       XP_076545621            3706 aa            linear   INV 03-SEP-2025
DEFINITION  terribly reduced optic lobes isoform X22 [Osmia lignaria lignaria].
ACCESSION   XP_076545621
VERSION     XP_076545621.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689506.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3706
                     /product="terribly reduced optic lobes isoform X22"
                     /calculated_mol_wt=407492
     Region          183..272
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     Region          304..337
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(308,316,327..328)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(320,323,327,333..334)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            330..334
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          434..468
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(439,447,458..459)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(451,454,458,464..465)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            461..465
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          473..507
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(478,486,497..498)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(490,493,497,503..504)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            500..504
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          509..543
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(514,522,533..534)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(526,529,533,539..540)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            536..540
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          549..583
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(554,562,573..574)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(566,569,573,579..580)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            576..580
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          588..622
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(593,601,612..613)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(605,608,612,618..619)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            615..619
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          624..658
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(629,637,648..649)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(641,644,648,654..655)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            651..655
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          663..697
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(668,676,687..688)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(680,683,687,693..694)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            690..694
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          700..733
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(706,715,726..727)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(719,722,726,732..733)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            729..733
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          764..797
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(768,776,787..788)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(780,783,787,793..794)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            790..794
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          800..834
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(805,813,824..825)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(817,820,824,830..831)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            827..831
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          838..872
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(843,851,862..863)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(855,858,862,868..869)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            865..869
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          874..908
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(879,887,898..899)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(891,894,898,904..905)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            901..905
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          922..956
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(927,935,946..947)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(939,942,946,952..953)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            949..953
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          960..991
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(965,973,984..985)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(977,980,984,990..991)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            987..991
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1007..1042
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1012,1021,1032..1033)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1025,1028,1032,1038..1039)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1035..1039
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1067..1143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          1073..1077
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1086..1090
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1106..1110
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1157..1191
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1162,1170,1181..1182)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1174,1177,1181,1187..1188)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1184..1188
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1196..1230
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1201,1209,1220..1221)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1213,1216,1220,1226..1227)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1223..1227
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1239..1274
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1244,1253,1264..1265)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1257,1260,1264,1270..1271)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1267..1271
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1292..1367
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1295..1299
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1308..1312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1331..1335
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1345..1350
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1359..1362
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1469..1603
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1659..1712
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1660,1662,1675,1685,1687,1696)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1719..1765
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1720,1730,1737,1739,1748)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1836..1972
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2007..2055
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2007,2009,2016,2023,2026,2035)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2062..2108
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(2062,2064,2074,2079,2081,2090)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2178..2314
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          2411..2492
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2422..2426
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2437..2441
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2458..2462
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2506..2583
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2517..2521
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2530..2534
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2549..2553
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2563..2568
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2576..2579
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2601..2680
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2612..2616
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2625..2629
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2645..2649
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2659..2664
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2672..2675
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2693..2771
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2704..2708
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2717..2721
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2736..2741
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2751..2756
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2767
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2788..2864
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2795..2799
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2809..2813
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2830..2834
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2844..2849
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2857..2860
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2879..2962
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          2887..2891
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2900..2904
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2924..2932
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2942..2947
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2955..2958
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2986..3065
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3002..3006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3016..3021
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3037..3041
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3051..3056
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3059..3062
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3077..3160
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3094..3098
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3107..3111
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3126..3130
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3140..3145
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3153..3156
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3175..3250
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3186..3190
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409362"
     Region          3197..3201
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409362"
     Region          3216..3220
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409362"
     Region          3230..3235
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409362"
     Region          3243..3246
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409362"
     Region          3267..3340
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3273..3277
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3286..3290
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3306..3310
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3320..3325
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3333..3336
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3358..3427
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          3361..3365
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3374..3378
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3394..3397
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3407..3412
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3420..3423
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3433..3585
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3608..3641
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     CDS             1..3706
                     /gene="trol"
                     /coded_by="XM_076689506.1:322..11442"
                     /db_xref="GeneID:117606665"
ORIGIN      
        1 mrnsgtsrcl lllliaaaal vdasqnddlv fdqdgkqsfn eapliethqq esmlhrlkrg
       61 lfswlqpass taaptteata dqdednaeee lenappgepd geaepkmssv kepanlerlv
      121 hergdqyedv deeneignta egrrepadfg ntddedlags gevegsatdg gidnhpsarg
      181 nrkyyritlt vgepyrreya drnsreykel sgnltqplee lynrripnen hhvnvvkisp
      241 tsdsftsqvt ldigstftde levrnileqq lqyhslgniq vgpegftfrv fqadsgvdel
      301 ecdqstelrc rdgacvplds rcdgiaqced gsdeldcsrs pfdatssllh fhpidrdhkt
      361 vtepyqepee svesttnqde eegitsrpsv nkcraddtvr csdgsrsics vqrcdgvpdc
      421 ddggdevgcp hpgcsagefa cdvsrciles hrcnfikecd dgsdehdcny pactstqfkc
      481 rngqcidssa hcngvedcrd rtdelncpcr edqfectpgf cvplnrrcdr fmdctngrde
      541 encsnmtrcr pdeftcsdgg cvsqnarcdg rsdcpdrsde hncnvttcsg dqfrcldgtc
      601 isidkrcnsn idcrngeden qcgcgeakfr ctdgqcigye lqcngveecs dgsderdcdl
      661 apcpsmdftc sdgsciskss vcdgfddcpr aedelhcere ctptqfkcvt gnkciegvyr
      721 cdghpdcpdh sdedcaneti thaiqptsqp wpgratdslr ecdpsremrc ddgkcvllrr
      781 kcdnifdcid gsdergcgvc tpaewkcasg ecipetercd nvvqcadgtd eagcvnecpa
      841 gmfrcndglc ldskrrcdgr phcldgsdei ncqcpdgelp cdngvcinkn ffcdrnadch
      901 dgsderdcpv idettdgdhv acrqdefacp hggcipqsav cngrndcpye edeincprgc
      961 gkdqfqcsng dciradqkcn gyidcvdgsd epeecerpgf hpmpgrcpag yimcvsdkdc
     1021 vpqssmcngi pecrdrsdee nceqlkepsr lnlktypseq vikenpakqg revvfqcrde
     1081 gplrarvrwl rgnnlplppg srdlngrlei pniqldhggl yiceavgypa sttgqqvnvy
     1141 ltvekfeppa tnkphvcmyd eatcsngdci pksyvcngrl dctdgsdemr csphgcepne
     1201 frcnntqcvs klwrcdgdkd cadgsdeenc vpnrpgspcr vteytcasnd qcipksyhcd
     1261 mekdcidgsd eigcspvyim kppppmitld lgttlvivct aigvptpein wrlnwghipp
     1321 kctmtsingt gtltcpdiqt edqgayscea lntagfvfav pdaivmvnkp ddvcpkgmfn
     1381 seartveeci scfcfgvate chsanlftyq ipppfdrhki vavkqepdlr ilndisyqla
     1441 vkplgrdglh ldestpflnq laaekdvpyf alpenfhgsq lksyggylky rvryngtgvp
     1501 nsapavvlsg nnyvlihkgk qlipnydnde svrffegewl kkqgwsevpa tredimmtla
     1561 nvdnilikve ydnspdldvq lthivmdtad vrntglgsas fveecqcpsg ytglsceeca
     1621 pgylrrktgl wlgqcyrdep vdcppgyygd psrnipckac pcpltnpsnq fartchlgsd
     1681 gqptcncppg yigrrceqcd vgyqgnplip gdmcvarqqc dpegsissvv dpltgkcrck
     1741 qyatgitcnq ckantfknqf gctscfcmgi tnkcvssnwy rndvrvsftn sirdfslies
     1801 rtphpppivd girlntvtre iiyndfpnrg nndvyywqlp siflgdqvts yggnlkytvr
     1861 yvpapggqss knnaadveli sandinllyf srespepntp qtftvplleq ywqrndgtqa
     1921 drehllmala dvsairikat ytthtdeaal slvsldiaek yntgrarave veecscpigy
     1981 kglscedcdv gytrasegly lgiceqcncn ghsaqcnpdt gicencadht tgdfcevcep
     2041 gyegdatrgt phdcvykdst pcrcneagsr ssscigdrcd ckrnvegpqc nrcrpstfgl
     2101 ssynpdgcte cycsgvtnqc hesslyvqqi pvwvydnqhg ftltdstrqe iiddgfelnf
     2161 amneigyryp dsrgrrlfws lpaiftgnkv ksyggnltlt qhitaypgaq sykdqdiili
     2221 gngitlfwtn pmdiqpdipl tysvplrese wrrlttegpr vasridlmtv lsnleailvr
     2281 athsermtat yisdisldta vehqtgnrra vqvevcrcpt ghvgtscesc argyyrdpnd
     2341 rsasylgscn pcpcnnnees ceitrsgqvk chclpgytgq yclstetnvt tttpyppyip
     2401 pprilvyikg pefqiveagt tvryhcagks ldngslhikw ekeggqlptg rtvddsqgll
     2461 virdvkvsds gvyvcqvsdg vhigyknvtl tvggsnpvap ravvmptsle vvegqpaefr
     2521 cmatghpqpq vewirvhgpt spevvthngi wslraasknd aaeykciarn nvgvdeqtvi
     2581 lyvsdnpsmp pttgilptit psqwygtegd avlltcsqts pelaslvwlr qgnlpfspta
     2641 tqrdgvltim nstqydsgvy iclattyvgt hvnntaeitv vprrsppsik vrpekqtvpq
     2701 gsvaevqcvt sgepglqvkw skyhherlss nvqqvgdtlr ivniqipdrg vyvchvtgps
     2761 gshessavie vepreaprve iyprevppvi lggstdlqcr avagipmpel hwshqngrpf
     2821 psnikqlpgg vlrlsnitan dggayvcsav npvgsntavv yievqsvpvi misprsgilq
     2881 vkrgdrvkli crssgypqpr vawvkhqnri dfrtpfylss eaatpleavy einsaspdde
     2941 gsytchanna agmveervyi rvednevysp deypgpdkpe npddqkitvp kdyfkipsgg
     3001 kvemrcyvrs sdgnhiyldw krtdhrplpe gstvhngiln ipvadksaag eyvclgmdqa
     3061 rnvlfraksh levlsppriv lnpprqtvra genpsidcst tgdepmtiqw aaigrnlpss
     3121 vtqdrgvlqf hgitysdagk yvctatndag taeavaevlv nehpfdessv vhaaqrdvvt
     3181 yagqpvrlrc tvnvratihw srdgqplpst arieddylel prarpedsgr yicqiqtvhg
     3241 vssdyinlnv sptvtpavsv easqnpvnig dtvnircacs gthnpryhwv rpnhpslpen
     3301 aqeygnvlrl tnvavsdsgv yrciadtpeg vfdhdfnlvv hdgnndapai etkyapygss
     3361 lemdckpnln pplkfhwskl ggllprdaqi fenklklinv kaedagtyvc tatnnlenie
     3421 iptvlvvtgv vpyfsqapes fialpplpds ylkfnieisf kpesydgiil yndesnrgng
     3481 dfillslvgg ypvfsfdlgs gptiiraeks vtlsewhtik lqrnrkegtm lvdgegpfkg
     3541 vaagrkqgld lkallfvggv psdntvnkya eitsgfvgci srlvigekei dliddqtnsv
     3601 gitncetcae npcnnagvcq eaatkngytc lcragysgkh cdyvgqscyp gacgegtcvd
     3661 ketgfecycp hgktgsrceh sltiyepafh ddksfvahet pkalrr
//
LOCUS       XP_034185473            1369 aa            linear   INV 03-SEP-2025
DEFINITION  disintegrin and metalloproteinase domain-containing protein
            mind-meld isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185473
VERSION     XP_034185473.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329582.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1369
                     /product="disintegrin and metalloproteinase
                     domain-containing protein mind-meld isoform X1"
                     /calculated_mol_wt=154246
     Region          122..229
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          277..479
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(415..416,419,425)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          496..570
                     /region_name="Disintegrin"
                     /note="pfam00200"
                     /db_xref="CDD:459709"
     Region          575..715
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     Region          733..757
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     CDS             1..1369
                     /gene="mmd"
                     /coded_by="XM_034329582.2:729..4838"
                     /db_xref="GeneID:117606738"
ORIGIN      
        1 mfwlhcglfv lviavpgfis sadstskrea dytlddsfwn eetpsgever llreyrqnqe
       61 lvrnigahfy qiiypvqlrh hekmgistre vsvskfpqrg ygeggyqnsr grmkfpsifn
      121 fqtgrhfhrt sllikafnhk frldlelntq llapnimqkd yladgveqss kqeiehcyyh
      181 gtvrdypgas aafhtcngvs gvihlgnetf vihpfyggdl sqkhphvife artkankgca
      241 nlgnlewrak srrqkhvlgl setpsdrykr dvrettkyie taiiidkamf ekrngstrae
      301 vvhdaiqvan iadlyfrtln trvsvtyiet wqstnqadis kgmdisialr nlndytvrrm
      361 yqvdedttql ltgeifagge lgmaeyltic kqkavgisvd lnvyephlla gtmahmighn
      421 igmahddrrs ecicrdwhgc imaqsivgle nvqpykfsec skmdyidslr agkgfcllnk
      481 pnevemrtsc gnkvidddee cdcgsidecp kidpccdpit ckltseaeca sgpccsnckl
      541 lkrgvvcrds tnecdlpevc sgesgqcpvd vykkngipcs ndqgycfnge cpaldlqcdq
      601 iwgyggvvad kqcfeqfnsk gsltghcgtd asghyikcep envrcgslqc qqgskqpvmg
      661 gmdhsrtiis ikgkeyecks ttgkvegsem prmglvrdgt scgdnlicvn qtctsrfpli
      721 dkehcpsnhn nnecsgngvc tnankcycyp gwsgpdcsiv qeipthpptt sstessgkns
      781 tdpklvkket pyenyhgsnt vflvgmlmsv vggvfivfal malcyrsvvv hknyslclrr
      841 kstvqkydqp ygkkapqksy senhrpehai lddvnkilts nsmpnysrsd nqrvvfrspn
      901 nvttadgprl kdhrsqakrl ainevdavts aeeavsfidl pnhptkkgil kkhpdygieg
      961 gtvvelertl drlngyhegi iealrnaesh relsgpplvn rnlidqealh kplsecsypd
     1021 pyrkgldrqe hmenqadeed eeeeeeeede smlpcgtiri rnlediikql erhttrnisr
     1081 cnsddirmse seadrhyrid ssvcsessqg srrcsrardd tygrycqpss rspygthqlv
     1141 qhphqmhkee diyetadpdr gsntrgetpd sesdafiqaq qqlarwtsed gvqhrppqqp
     1201 pppppppppp amtggtvpll qrrqsqrdyr qqqqqqhhhh hqsqplqqqs sqqqhqhhqq
     1261 eqqqqqhqlp veqlpiqqrg yypsplhtdn gldndetena qshqkqmlpd vrgidvnhlp
     1321 ninkcplddn fsldcninng slkelntnnt sdnentallp pshfpeykh
//
LOCUS       XP_034185474            1368 aa            linear   INV 03-SEP-2025
DEFINITION  disintegrin and metalloproteinase domain-containing protein
            mind-meld isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185474
VERSION     XP_034185474.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329583.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1368
                     /product="disintegrin and metalloproteinase
                     domain-containing protein mind-meld isoform X2"
                     /calculated_mol_wt=154118
     Region          122..228
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          276..478
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(414..415,418,424)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          495..569
                     /region_name="Disintegrin"
                     /note="pfam00200"
                     /db_xref="CDD:459709"
     Region          574..714
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     Region          732..756
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     CDS             1..1368
                     /gene="mmd"
                     /coded_by="XM_034329583.2:729..4835"
                     /db_xref="GeneID:117606738"
ORIGIN      
        1 mfwlhcglfv lviavpgfis sadstskrea dytlddsfwn eetpsgever llreyrqnqe
       61 lvrnigahfy qiiypvqlrh hekmgistre vsvskfpqrg ygeggyqnsr grmkfpsifn
      121 fqtgrhfhrt sllikafnhk frldlelntq llapnimqkd yladgveqss kqeiehcyyh
      181 gtvrdypgas aafhtcngvs gvihlgnetf vihpfyggdl skhphvifea rtkankgcan
      241 lgnlewraks rrqkhvlgls etpsdrykrd vrettkyiet aiiidkamfe krngstraev
      301 vhdaiqvani adlyfrtlnt rvsvtyietw qstnqadisk gmdisialrn lndytvrrmy
      361 qvdedttqll tgeifaggel gmaeyltick qkavgisvdl nvyephllag tmahmighni
      421 gmahddrrse cicrdwhgci maqsivglen vqpykfsecs kmdyidslra gkgfcllnkp
      481 nevemrtscg nkvidddeec dcgsidecpk idpccdpitc kltseaecas gpccsnckll
      541 krgvvcrdst necdlpevcs gesgqcpvdv ykkngipcsn dqgycfngec paldlqcdqi
      601 wgyggvvadk qcfeqfnskg sltghcgtda sghyikcepe nvrcgslqcq qgskqpvmgg
      661 mdhsrtiisi kgkeyeckst tgkvegsemp rmglvrdgts cgdnlicvnq tctsrfplid
      721 kehcpsnhnn necsgngvct nankcycypg wsgpdcsivq eipthpptts stessgknst
      781 dpklvkketp yenyhgsntv flvgmlmsvv ggvfivfalm alcyrsvvvh knyslclrrk
      841 stvqkydqpy gkkapqksys enhrpehail ddvnkiltsn smpnysrsdn qrvvfrspnn
      901 vttadgprlk dhrsqakrla inevdavtsa eeavsfidlp nhptkkgilk khpdygiegg
      961 tvvelertld rlngyhegii ealrnaeshr elsgpplvnr nlidqealhk plsecsypdp
     1021 yrkgldrqeh menqadeede eeeeeeedes mlpcgtirir nlediikqle rhttrnisrc
     1081 nsddirmses eadrhyrids svcsessqgs rrcsrarddt ygrycqpssr spygthqlvq
     1141 hphqmhkeed iyetadpdrg sntrgetpds esdafiqaqq qlarwtsedg vqhrppqqpp
     1201 pppppppppa mtggtvpllq rrqsqrdyrq qqqqqhhhhh qsqplqqqss qqqhqhhqqe
     1261 qqqqqhqlpv eqlpiqqrgy ypsplhtdng ldndetenaq shqkqmlpdv rgidvnhlpn
     1321 inkcplddnf sldcninngs lkelntnnts dnentallpp shfpeykh
//
LOCUS       XP_034185475            1361 aa            linear   INV 03-SEP-2025
DEFINITION  disintegrin and metalloproteinase domain-containing protein
            mind-meld isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185475
VERSION     XP_034185475.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329584.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1361
                     /product="disintegrin and metalloproteinase
                     domain-containing protein mind-meld isoform X3"
                     /calculated_mol_wt=153265
     Region          120..221
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          269..471
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(407..408,411,417)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          488..562
                     /region_name="Disintegrin"
                     /note="pfam00200"
                     /db_xref="CDD:459709"
     Region          567..707
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     Region          725..749
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     CDS             1..1361
                     /gene="mmd"
                     /coded_by="XM_034329584.2:729..4814"
                     /db_xref="GeneID:117606738"
ORIGIN      
        1 mfwlhcglfv lviavpgfis sadstskrea dytlddsfwn eetpsgever llreyrqnqe
       61 lvrnigahfy qiiypvqlrh hekmgistre vsvskfpqrg ygeggyqnsr grmktgrhfh
      121 rtsllikafn hkfrldleln tqllapnimq kdyladgveq sskqeiehcy yhgtvrdypg
      181 asaafhtcng vsgvihlgne tfvihpfygg dlsqkhphvi feartkankg canlgnlewr
      241 aksrrqkhvl glsetpsdry krdvrettky ietaiiidka mfekrngstr aevvhdaiqv
      301 aniadlyfrt lntrvsvtyi etwqstnqad iskgmdisia lrnlndytvr rmyqvdedtt
      361 qlltgeifag gelgmaeylt ickqkavgis vdlnvyephl lagtmahmig hnigmahddr
      421 rsecicrdwh gcimaqsivg lenvqpykfs ecskmdyids lragkgfcll nkpnevemrt
      481 scgnkviddd eecdcgside cpkidpccdp itckltseae casgpccsnc kllkrgvvcr
      541 dstnecdlpe vcsgesgqcp vdvykkngip csndqgycfn gecpaldlqc dqiwgyggvv
      601 adkqcfeqfn skgsltghcg tdasghyikc epenvrcgsl qcqqgskqpv mggmdhsrti
      661 isikgkeyec ksttgkvegs emprmglvrd gtscgdnlic vnqtctsrfp lidkehcpsn
      721 hnnnecsgng vctnankcyc ypgwsgpdcs ivqeipthpp ttsstessgk nstdpklvkk
      781 etpyenyhgs ntvflvgmlm svvggvfivf almalcyrsv vvhknyslcl rrkstvqkyd
      841 qpygkkapqk sysenhrpeh ailddvnkil tsnsmpnysr sdnqrvvfrs pnnvttadgp
      901 rlkdhrsqak rlainevdav tsaeeavsfi dlpnhptkkg ilkkhpdygi eggtvveler
      961 tldrlngyhe giiealrnae shrelsgppl vnrnlidqea lhkplsecsy pdpyrkgldr
     1021 qehmenqade edeeeeeeee desmlpcgti rirnlediik qlerhttrni srcnsddirm
     1081 seseadrhyr idssvcsess qgsrrcsrar ddtygrycqp ssrspygthq lvqhphqmhk
     1141 eediyetadp drgsntrget pdsesdafiq aqqqlarwts edgvqhrppq qppppppppp
     1201 ppamtggtvp llqrrqsqrd yrqqqqqqhh hhhqsqplqq qssqqqhqhh qqeqqqqqhq
     1261 lpveqlpiqq rgyypsplht dngldndete naqshqkqml pdvrgidvnh lpninkcpld
     1321 dnfsldcnin ngslkelntn ntsdnental lppshfpeyk h
//
LOCUS       XP_034185476            1355 aa            linear   INV 03-SEP-2025
DEFINITION  disintegrin and metalloproteinase domain-containing protein
            mind-meld isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185476
VERSION     XP_034185476.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329585.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1355
                     /product="disintegrin and metalloproteinase
                     domain-containing protein mind-meld isoform X4"
                     /calculated_mol_wt=152618
     Region          122..228
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          276..478
                     /region_name="ZnMc_adamalysin_II_like"
                     /note="Zinc-dependent metalloprotease; adamalysin_II_like
                     subfamily. Adamalysin II is a snake venom zinc
                     endopeptidase. This subfamily contains other snake venom
                     metalloproteinases, as well as membrane-anchored
                     metalloproteases belonging to the ADAM family; cd04269"
                     /db_xref="CDD:239797"
     Site            order(414..415,418,424)
                     /site_type="active"
                     /db_xref="CDD:239797"
     Region          495..569
                     /region_name="Disintegrin"
                     /note="pfam00200"
                     /db_xref="CDD:459709"
     Region          574..714
                     /region_name="ACR"
                     /note="ADAM Cysteine-Rich Domain; smart00608"
                     /db_xref="CDD:214743"
     Region          732..756
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     CDS             1..1355
                     /gene="mmd"
                     /coded_by="XM_034329585.2:729..4796"
                     /db_xref="GeneID:117606738"
ORIGIN      
        1 mfwlhcglfv lviavpgfis sadstskrea dytlddsfwn eetpsgever llreyrqnqe
       61 lvrnigahfy qiiypvqlrh hekmgistre vsvskfpqrg ygeggyqnsr grmkfpsifn
      121 fqtgrhfhrt sllikafnhk frldlelntq llapnimqkd yladgveqss kqeiehcyyh
      181 gtvrdypgas aafhtcngvs gvihlgnetf vihpfyggdl skhphvifea rtkankgcan
      241 lgnlewraks rrqkhvlgls etpsdrykrd vrettkyiet aiiidkamfe krngstraev
      301 vhdaiqvani adlyfrtlnt rvsvtyietw qstnqadisk gmdisialrn lndytvrrmy
      361 qvdedttqll tgeifaggel gmaeyltick qkavgisvdl nvyephllag tmahmighni
      421 gmahddrrse cicrdwhgci maqsivglen vqpykfsecs kmdyidslra gkgfcllnkp
      481 nevemrtscg nkvidddeec dcgsidecpk idpccdpitc kltseaecas gpccsnckll
      541 krgvvcrdst necdlpevcs gesgqcpvdv ykkngipcsn dqgycfngec paldlqcdqi
      601 wgyggvvadk qcfeqfnskg sltghcgtda sghyikcepe nvrcgslqcq qgskqpvmgg
      661 mdhsrtiisi kgkeyeckst tgkvegsemp rmglvrdgts cgdnlicvnq tctsrfplid
      721 kehcpsnhnn necsgngvct nankcycypg wsgpdcsivq eipthpptts stessgknst
      781 dpklvkketp yenyhgsntv flvgmlmsvv ggvfivfalm alcyrrkstv qkydqpygkk
      841 apqksysenh rpehailddv nkiltsnsmp nysrsdnqrv vfrspnnvtt adgprlkdhr
      901 sqakrlaine vdavtsaeea vsfidlpnhp tkkgilkkhp dygieggtvv elertldrln
      961 gyhegiieal rnaeshrels gpplvnrnli dqealhkpls ecsypdpyrk gldrqehmen
     1021 qadeedeeee eeeedesmlp cgtirirnle diikqlerht trnisrcnsd dirmsesead
     1081 rhyridssvc sessqgsrrc srarddtygr ycqpssrspy gthqlvqhph qmhkeediye
     1141 tadpdrgsnt rgetpdsesd afiqaqqqla rwtsedgvqh rppqqppppp ppppppamtg
     1201 gtvpllqrrq sqrdyrqqqq qqhhhhhqsq plqqqssqqq hqhhqqeqqq qqhqlpveql
     1261 piqqrgyyps plhtdngldn detenaqshq kqmlpdvrgi dvnhlpnink cplddnfsld
     1321 cninngslke lntnntsdne ntallppshf peykh
//
LOCUS       XP_034185478             752 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein dilatory isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185478
VERSION     XP_034185478.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329587.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185478.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="centrosomal protein dilatory isoform X1"
                     /calculated_mol_wt=87778
     Region          <250..>742
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..752
                     /gene="dila"
                     /coded_by="XM_034329587.2:1..2259"
                     /db_xref="GeneID:117606739"
ORIGIN      
        1 mgancnsgfl pavtaatpsf rsislsvyvq tpspspspsp sasaspspvq rdsspattsp
       61 harlslptdd hcllvraaif plvsrfqssr lpprrcclqp sanprrrrrq qrsshpktrg
      121 kppvnrpnav qekadcnqnv ecsfeqlcnm indleknivn empevenhlq lepekdnsdg
      181 tcggngttyd dimtfltkfe etgpidekld mvevdaetml remleepqgl pclscdsanv
      241 ennspytfke dlatarlqle eknatvsllk eqlrserkla ceklesqkrn sacklqqqed
      301 rykgivkrhq kfieqlitek teltekcnsl vqrvkeietk mqrdlkaaae rhavelqrak
      361 dryaaaekik rerwieartt kikemtvkgl eselhnmmeq haeeiqrlrn vhmkelqdve
      421 lrtirrsneq leqlrlelta sqermmtnek nllwtrykek leeqesqfke qqgklleelq
      481 lerenlsker gkweaekeaa lqqmhlryqe etellkqrhl nekkvveesl kmeweawlad
      541 ykrqqnlrie qaeskireec dkerdrqiel vierfekhsr dakltlqqnf drnlrywrek
      601 letslqaavd aersskdklt ltkeklekve iqlqqtenkl rqclselnda neviqqlrme
      661 kdqakklarq eiegekrele ekiaslyqei trinsnrdas msqlhsrikl imtqkvltik
      721 nltkelddak rqsehlekll dqqrreyilk sl
//
LOCUS       XP_034185479             643 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein dilatory isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185479
VERSION     XP_034185479.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329588.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185479.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..643
                     /product="centrosomal protein dilatory isoform X2"
                     /calculated_mol_wt=76169
     Region          <141..>633
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..643
                     /gene="dila"
                     /coded_by="XM_034329588.2:153..2084"
                     /db_xref="GeneID:117606739"
ORIGIN      
        1 minikhpktr gkppvnrpna vqekadcnqn vecsfeqlcn mindlekniv nempevenhl
       61 qlepekdnsd gtcggngtty ddimtfltkf eetgpidekl dmvevdaetm lremleepqg
      121 lpclscdsan vennspytfk edlatarlql eeknatvsll keqlrserkl aceklesqkr
      181 nsacklqqqe drykgivkrh qkfieqlite kteltekcns lvqrvkeiet kmqrdlkaaa
      241 erhavelqra kdryaaaeki krerwieart tkikemtvkg leselhnmme qhaeeiqrlr
      301 nvhmkelqdv elrtirrsne qleqlrlelt asqermmtne knllwtryke kleeqesqfk
      361 eqqgklleel qlerenlske rgkweaekea alqqmhlryq eetellkqrh lnekkvvees
      421 lkmeweawla dykrqqnlri eqaeskiree cdkerdrqie lvierfekhs rdakltlqqn
      481 fdrnlrywre kletslqaav daersskdkl tltkeklekv eiqlqqtenk lrqclselnd
      541 aneviqqlrm ekdqakklar qeiegekrel eekiaslyqe itrinsnrda smsqlhsrik
      601 limtqkvlti knltkeldda krqsehlekl ldqqrreyil ksl
//
LOCUS       XP_034185480             542 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein dilatory isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185480
VERSION     XP_034185480.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329589.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185480.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="centrosomal protein dilatory isoform X3"
                     /calculated_mol_wt=64763
     Region          63..>476
                     /region_name="PLN02939"
                     /note="transferase, transferring glycosyl groups"
                     /db_xref="CDD:215507"
     CDS             1..542
                     /gene="dila"
                     /coded_by="XM_034329589.2:131..1759"
                     /db_xref="GeneID:117606739"
ORIGIN      
        1 mvevdaetml remleepqgl pclscdsanv ennspytfke dlatarlqle eknatvsllk
       61 eqlrserkla ceklesqkrn sacklqqqed rykgivkrhq kfieqlitek teltekcnsl
      121 vqrvkeietk mqrdlkaaae rhavelqrak dryaaaekik rerwieartt kikemtvkgl
      181 eselhnmmeq haeeiqrlrn vhmkelqdve lrtirrsneq leqlrlelta sqermmtnek
      241 nllwtrykek leeqesqfke qqgklleelq lerenlsker gkweaekeaa lqqmhlryqe
      301 etellkqrhl nekkvveesl kmeweawlad ykrqqnlrie qaeskireec dkerdrqiel
      361 vierfekhsr dakltlqqnf drnlrywrek letslqaavd aersskdklt ltkeklekve
      421 iqlqqtenkl rqclselnda neviqqlrme kdqakklarq eiegekrele ekiaslyqei
      481 trinsnrdas msqlhsrikl imtqkvltik nltkelddak rqsehlekll dqqrreyilk
      541 sl
//
LOCUS       XP_034185482             542 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein dilatory isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185482
VERSION     XP_034185482.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329591.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185482.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="centrosomal protein dilatory isoform X3"
                     /calculated_mol_wt=64763
     Region          63..>476
                     /region_name="PLN02939"
                     /note="transferase, transferring glycosyl groups"
                     /db_xref="CDD:215507"
     CDS             1..542
                     /gene="dila"
                     /coded_by="XM_034329591.2:138..1766"
                     /db_xref="GeneID:117606739"
ORIGIN      
        1 mvevdaetml remleepqgl pclscdsanv ennspytfke dlatarlqle eknatvsllk
       61 eqlrserkla ceklesqkrn sacklqqqed rykgivkrhq kfieqlitek teltekcnsl
      121 vqrvkeietk mqrdlkaaae rhavelqrak dryaaaekik rerwieartt kikemtvkgl
      181 eselhnmmeq haeeiqrlrn vhmkelqdve lrtirrsneq leqlrlelta sqermmtnek
      241 nllwtrykek leeqesqfke qqgklleelq lerenlsker gkweaekeaa lqqmhlryqe
      301 etellkqrhl nekkvveesl kmeweawlad ykrqqnlrie qaeskireec dkerdrqiel
      361 vierfekhsr dakltlqqnf drnlrywrek letslqaavd aersskdklt ltkeklekve
      421 iqlqqtenkl rqclselnda neviqqlrme kdqakklarq eiegekrele ekiaslyqei
      481 trinsnrdas msqlhsrikl imtqkvltik nltkelddak rqsehlekll dqqrreyilk
      541 sl
//
LOCUS       XP_034185483             542 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein dilatory isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185483
VERSION     XP_034185483.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329592.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185483.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="centrosomal protein dilatory isoform X3"
                     /calculated_mol_wt=64763
     Region          63..>476
                     /region_name="PLN02939"
                     /note="transferase, transferring glycosyl groups"
                     /db_xref="CDD:215507"
     CDS             1..542
                     /gene="dila"
                     /coded_by="XM_034329592.2:122..1750"
                     /db_xref="GeneID:117606739"
ORIGIN      
        1 mvevdaetml remleepqgl pclscdsanv ennspytfke dlatarlqle eknatvsllk
       61 eqlrserkla ceklesqkrn sacklqqqed rykgivkrhq kfieqlitek teltekcnsl
      121 vqrvkeietk mqrdlkaaae rhavelqrak dryaaaekik rerwieartt kikemtvkgl
      181 eselhnmmeq haeeiqrlrn vhmkelqdve lrtirrsneq leqlrlelta sqermmtnek
      241 nllwtrykek leeqesqfke qqgklleelq lerenlsker gkweaekeaa lqqmhlryqe
      301 etellkqrhl nekkvveesl kmeweawlad ykrqqnlrie qaeskireec dkerdrqiel
      361 vierfekhsr dakltlqqnf drnlrywrek letslqaavd aersskdklt ltkeklekve
      421 iqlqqtenkl rqclselnda neviqqlrme kdqakklarq eiegekrele ekiaslyqei
      481 trinsnrdas msqlhsrikl imtqkvltik nltkelddak rqsehlekll dqqrreyilk
      541 sl
//
LOCUS       XP_034185484             516 aa            linear   INV 03-SEP-2025
DEFINITION  DNA fragmentation factor-related protein 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185484
VERSION     XP_034185484.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329593.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="DNA fragmentation factor-related protein 2
                     isoform X1"
                     /calculated_mol_wt=58166
     Region          10..84
                     /region_name="CIDE-N"
                     /note="CIDE-N domain; pfam02017"
                     /db_xref="CDD:460413"
     CDS             1..516
                     /gene="Drep2"
                     /coded_by="XM_034329593.2:131..1681"
                     /db_xref="GeneID:117606740"
ORIGIN      
        1 mareelrskr pfkiwdswrn vrkglvvsnf eelihrgkek lgvpqnenvs lvlesdgtqv
       61 edgeyfktla nntillllrh gerwfptgvd iiraaisaip kivcetihal elhdetpswk
      121 imdnkgrvtv vlhwdqrssa ssqvqqqqkv pdaqsskysp tkktdlsgsq rpslviqtsl
      181 dklnygpksg hlageigpsr ytspqitvin hddmqqqqqq pqpqphgipa rlvkqstnsf
      241 dsttihihtp ecsnhihqpv arngspvnga dggpsgecdf hccalheegr riavhksvat
      301 spiqdaqhqq yqqqhhhphp ssqqqqqqqq ppqqqqtqts spqppsslsd gtrrpglskg
      361 hvrfcdtade esssrlagns tggfhlhhhh nhhqehdsse setentimed eivtsekfll
      421 lidqltvdqk hlsikdigii lerlsskild verldreses eecynwtika iirgdvlrel
      481 gviyngnyya isehpgykee seennednee eeedrl
//
LOCUS       XP_034185485             515 aa            linear   INV 03-SEP-2025
DEFINITION  DNA fragmentation factor-related protein 2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185485
VERSION     XP_034185485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="DNA fragmentation factor-related protein 2
                     isoform X2"
                     /calculated_mol_wt=58038
     Region          10..84
                     /region_name="CIDE-N"
                     /note="CIDE-N domain; pfam02017"
                     /db_xref="CDD:460413"
     CDS             1..515
                     /gene="Drep2"
                     /coded_by="XM_034329594.2:131..1678"
                     /db_xref="GeneID:117606740"
ORIGIN      
        1 mareelrskr pfkiwdswrn vrkglvvsnf eelihrgkek lgvpqnenvs lvlesdgtqv
       61 edgeyfktla nntillllrh gerwfptgvd iiraaisaip kivcetihal elhdetpswk
      121 imdnkgrvtv vlhwdqrssa ssqvqqqqkv pdaqsskysp tkktdlsgsq rpslviqtsl
      181 dklnygpksg hlageigpsr ytspqitvin hddmqqqqqp qpqphgipar lvkqstnsfd
      241 sttihihtpe csnhihqpva rngspvngad ggpsgecdfh ccalheegrr iavhksvats
      301 piqdaqhqqy qqqhhhphps sqqqqqqqqp pqqqqtqtss pqppsslsdg trrpglskgh
      361 vrfcdtadee sssrlagnst ggfhlhhhhn hhqehdsses etentimede ivtsekflll
      421 idqltvdqkh lsikdigiil erlsskildv erldresese ecynwtikai irgdvlrelg
      481 viyngnyyai sehpgykees eennedneee eedrl
//
LOCUS       XP_076545622             503 aa            linear   INV 03-SEP-2025
DEFINITION  brahma-associated protein 60 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545622
VERSION     XP_076545622.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689507.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..503
                     /product="brahma-associated protein 60 isoform X1"
                     /calculated_mol_wt=57997
     Region          282..358
                     /region_name="SWIB_BAF60A"
                     /note="SWIB domain found in BRG1-associated factor 60A
                     (BAF60A) and similar proteins; cd17674"
                     /db_xref="CDD:349493"
     Site            order(301,304..305,307..308,311..312,317,322..323,325,342,
                     345..346)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349493"
     CDS             1..503
                     /gene="Bap60"
                     /coded_by="XM_076689507.1:163..1674"
                     /db_xref="GeneID:117606741"
ORIGIN      
        1 maqrfpvpnt gnngpppqry aassvppnlr qysgpnfpmq qrsgftpppq matagpgtas
       61 iiranqpysn mrqgpmptpp vgkrsadqri pmsqqkpyfw nsdfshstsk kkkkladkil
      121 pqkvrdlvpe sqaymdllaf erkldatimr krldiqealk rpmkqkrklr ifisntfypa
      181 keagegeegs vaswelrveg rllddtkndp nkvksnvkrk fssffkslvi eldkdlygpd
      241 nhlvewhrtl ttqetdgfqv krpgdknvrc tilllldyqp lqfkldprla rllgvhtqtr
      301 pviisalwqy ikthklqdsh erefincdky leqifacsrm kfaeipqrln pllhppdpiv
      361 inhvisvegt etkqtacydi dvevddtlkt qmnnfllsta sqqeiqsldn kihetvetin
      421 qlktnreffl sfakdpqqfi nkwiisqtrd lktmidvvgn peeerraefy yqpwaqeavc
      481 ryfytkvqqk raeleqalgi rns
//
LOCUS       XP_076545623             503 aa            linear   INV 03-SEP-2025
DEFINITION  brahma-associated protein 60 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545623
VERSION     XP_076545623.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689508.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..503
                     /product="brahma-associated protein 60 isoform X1"
                     /calculated_mol_wt=57997
     Region          282..358
                     /region_name="SWIB_BAF60A"
                     /note="SWIB domain found in BRG1-associated factor 60A
                     (BAF60A) and similar proteins; cd17674"
                     /db_xref="CDD:349493"
     Site            order(301,304..305,307..308,311..312,317,322..323,325,342,
                     345..346)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349493"
     CDS             1..503
                     /gene="Bap60"
                     /coded_by="XM_076689508.1:358..1869"
                     /db_xref="GeneID:117606741"
ORIGIN      
        1 maqrfpvpnt gnngpppqry aassvppnlr qysgpnfpmq qrsgftpppq matagpgtas
       61 iiranqpysn mrqgpmptpp vgkrsadqri pmsqqkpyfw nsdfshstsk kkkkladkil
      121 pqkvrdlvpe sqaymdllaf erkldatimr krldiqealk rpmkqkrklr ifisntfypa
      181 keagegeegs vaswelrveg rllddtkndp nkvksnvkrk fssffkslvi eldkdlygpd
      241 nhlvewhrtl ttqetdgfqv krpgdknvrc tilllldyqp lqfkldprla rllgvhtqtr
      301 pviisalwqy ikthklqdsh erefincdky leqifacsrm kfaeipqrln pllhppdpiv
      361 inhvisvegt etkqtacydi dvevddtlkt qmnnfllsta sqqeiqsldn kihetvetin
      421 qlktnreffl sfakdpqqfi nkwiisqtrd lktmidvvgn peeerraefy yqpwaqeavc
      481 ryfytkvqqk raeleqalgi rns
//
LOCUS       XP_076545624             503 aa            linear   INV 03-SEP-2025
DEFINITION  brahma-associated protein 60 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545624
VERSION     XP_076545624.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689509.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..503
                     /product="brahma-associated protein 60 isoform X1"
                     /calculated_mol_wt=57997
     Region          282..358
                     /region_name="SWIB_BAF60A"
                     /note="SWIB domain found in BRG1-associated factor 60A
                     (BAF60A) and similar proteins; cd17674"
                     /db_xref="CDD:349493"
     Site            order(301,304..305,307..308,311..312,317,322..323,325,342,
                     345..346)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349493"
     CDS             1..503
                     /gene="Bap60"
                     /coded_by="XM_076689509.1:124..1635"
                     /db_xref="GeneID:117606741"
ORIGIN      
        1 maqrfpvpnt gnngpppqry aassvppnlr qysgpnfpmq qrsgftpppq matagpgtas
       61 iiranqpysn mrqgpmptpp vgkrsadqri pmsqqkpyfw nsdfshstsk kkkkladkil
      121 pqkvrdlvpe sqaymdllaf erkldatimr krldiqealk rpmkqkrklr ifisntfypa
      181 keagegeegs vaswelrveg rllddtkndp nkvksnvkrk fssffkslvi eldkdlygpd
      241 nhlvewhrtl ttqetdgfqv krpgdknvrc tilllldyqp lqfkldprla rllgvhtqtr
      301 pviisalwqy ikthklqdsh erefincdky leqifacsrm kfaeipqrln pllhppdpiv
      361 inhvisvegt etkqtacydi dvevddtlkt qmnnfllsta sqqeiqsldn kihetvetin
      421 qlktnreffl sfakdpqqfi nkwiisqtrd lktmidvvgn peeerraefy yqpwaqeavc
      481 ryfytkvqqk raeleqalgi rns
//
LOCUS       XP_034185486             499 aa            linear   INV 03-SEP-2025
DEFINITION  brahma-associated protein 60 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185486
VERSION     XP_034185486.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329595.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..499
                     /product="brahma-associated protein 60 isoform X2"
                     /calculated_mol_wt=57568
     Region          278..354
                     /region_name="SWIB_BAF60A"
                     /note="SWIB domain found in BRG1-associated factor 60A
                     (BAF60A) and similar proteins; cd17674"
                     /db_xref="CDD:349493"
     Site            order(297,300..301,303..304,307..308,313,318..319,321,338,
                     341..342)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349493"
     CDS             1..499
                     /gene="Bap60"
                     /coded_by="XM_034329595.2:163..1662"
                     /db_xref="GeneID:117606741"
ORIGIN      
        1 maqrfpvpnt gnngpppqry aassvppnlr qysgpnfpmq qrsgftpppq matagpgtas
       61 iiranqpysn mrqgpmptpp vgkrsadqri pmsqqkpyfw nsdfshstsk kkkkladkil
      121 pqkvrdlvpe sqaymdllaf erkldatimr krldiqealk rpmkqkrklr ifisntfypa
      181 keagegeegs vaswelrveg rllddtkndp nkvkrkfssf fkslvieldk dlygpdnhlv
      241 ewhrtlttqe tdgfqvkrpg dknvrctill lldyqplqfk ldprlarllg vhtqtrpvii
      301 salwqyikth klqdsheref incdkyleqi facsrmkfae ipqrlnpllh ppdpivinhv
      361 isvegtetkq tacydidvev ddtlktqmnn fllstasqqe iqsldnkihe tvetinqlkt
      421 nrefflsfak dpqqfinkwi isqtrdlktm idvvgnpeee rraefyyqpw aqeavcryfy
      481 tkvqqkrael eqalgirns
//
LOCUS       XP_076545625             498 aa            linear   INV 03-SEP-2025
DEFINITION  brahma-associated protein 60 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545625
VERSION     XP_076545625.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689510.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="brahma-associated protein 60 isoform X3"
                     /calculated_mol_wt=57299
     Region          277..353
                     /region_name="SWIB_BAF60A"
                     /note="SWIB domain found in BRG1-associated factor 60A
                     (BAF60A) and similar proteins; cd17674"
                     /db_xref="CDD:349493"
     Site            order(296,299..300,302..303,306..307,312,317..318,320,337,
                     340..341)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349493"
     CDS             1..498
                     /gene="Bap60"
                     /coded_by="XM_076689510.1:163..1659"
                     /db_xref="GeneID:117606741"
ORIGIN      
        1 maqrfpvpnt gnngpppqry aassvppnlr qysgpnfpmq qrsgftpppq matagpgtas
       61 iiranqpysn mrqgpmptpp vgkrsadqri pmsqqkpdfs hstskkkkkl adkilpqkvr
      121 dlvpesqaym dllaferkld atimrkrldi qealkrpmkq krklrifisn tfypakeage
      181 geegsvaswe lrvegrlldd tkndpnkvks nvkrkfssff kslvieldkd lygpdnhlve
      241 whrtlttqet dgfqvkrpgd knvrctilll ldyqplqfkl dprlarllgv htqtrpviis
      301 alwqyikthk lqdsherefi ncdkyleqif acsrmkfaei pqrlnpllhp pdpivinhvi
      361 svegtetkqt acydidvevd dtlktqmnnf llstasqqei qsldnkihet vetinqlktn
      421 refflsfakd pqqfinkwii sqtrdlktmi dvvgnpeeer raefyyqpwa qeavcryfyt
      481 kvqqkraele qalgirns
//
LOCUS       XP_034185489             494 aa            linear   INV 03-SEP-2025
DEFINITION  brahma-associated protein 60 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185489
VERSION     XP_034185489.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329598.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="brahma-associated protein 60 isoform X4"
                     /calculated_mol_wt=56870
     Region          273..349
                     /region_name="SWIB_BAF60A"
                     /note="SWIB domain found in BRG1-associated factor 60A
                     (BAF60A) and similar proteins; cd17674"
                     /db_xref="CDD:349493"
     Site            order(292,295..296,298..299,302..303,308,313..314,316,333,
                     336..337)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349493"
     CDS             1..494
                     /gene="Bap60"
                     /coded_by="XM_034329598.2:163..1647"
                     /db_xref="GeneID:117606741"
ORIGIN      
        1 maqrfpvpnt gnngpppqry aassvppnlr qysgpnfpmq qrsgftpppq matagpgtas
       61 iiranqpysn mrqgpmptpp vgkrsadqri pmsqqkpdfs hstskkkkkl adkilpqkvr
      121 dlvpesqaym dllaferkld atimrkrldi qealkrpmkq krklrifisn tfypakeage
      181 geegsvaswe lrvegrlldd tkndpnkvkr kfssffkslv ieldkdlygp dnhlvewhrt
      241 lttqetdgfq vkrpgdknvr ctilllldyq plqfkldprl arllgvhtqt rpviisalwq
      301 yikthklqds herefincdk yleqifacsr mkfaeipqrl npllhppdpi vinhvisveg
      361 tetkqtacyd idvevddtlk tqmnnfllst asqqeiqsld nkihetveti nqlktnreff
      421 lsfakdpqqf inkwiisqtr dlktmidvvg npeeerraef yyqpwaqeav cryfytkvqq
      481 kraeleqalg irns
//
LOCUS       XP_034185490             367 aa            linear   INV 03-SEP-2025
DEFINITION  GTP-binding protein 128up [Osmia lignaria lignaria].
ACCESSION   XP_034185490
VERSION     XP_034185490.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329599.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..367
                     /product="GTP-binding protein 128up"
                     /calculated_mol_wt=40864
     Region          1..366
                     /region_name="Rbg1"
                     /note="Ribosome-interacting GTPase RBG1 [Translation,
                     ribosomal structure and biogenesis]; COG1163"
                     /db_xref="CDD:440777"
     CDS             1..367
                     /gene="128up"
                     /coded_by="XM_034329599.2:354..1457"
                     /db_xref="GeneID:117606742"
ORIGIN      
        1 mstilekias ieaemartqk nkatsghlgl lkaklaklrr elitpkgggg ggeqgfevak
       61 tgdarigfvg fpsvgkstll stlagvysev aayefttltt vpgcikykga kiqlldlpgi
      121 iegakdgkgr grqviavart cslifivldv lkplghkrli ehelegfglr lnkqppnitf
      181 rkkdkgginf qttcaqseld ldlvksilse yrihnaditl rydatsddli dviegnrvyv
      241 pciyllnkid qisieeldii ykiphcvpis ahhkwnfddl lekmwdylql vriytkpkgq
      301 lpdynspvvl nrerrtvedf cnklhrsiak efkyalvwgs svkhqpqkvg kdhvlcdedv
      361 vqivkkv
//
LOCUS       XP_076545626             530 aa            linear   INV 03-SEP-2025
DEFINITION  basement membrane-specific heparan sulfate proteoglycan core
            protein-like, partial [Osmia lignaria lignaria].
ACCESSION   XP_076545626
VERSION     XP_076545626.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689511.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..530
                     /product="basement membrane-specific heparan sulfate
                     proteoglycan core protein-like"
     Region          3..139
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          226..259
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          268..417
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     CDS             1..530
                     /gene="LOC117606625"
                     /coded_by="XM_076689511.1:<1..1593"
                     /db_xref="GeneID:117606625"
ORIGIN      
        1 hvlfycrlrv alnfnplddr dgiliycsqs deglgdfval tvknrriefr ydigsgvaii
       61 ksnhilqpgv wthvsitrdy kegnltvnge ptvtgkspgp srtmtlntpl yiggvdrrri
      121 ivnrnvsvnh afrgcisdlg vsslnvdilk saidsanidd cnvlhpnqtk ltpivttpps
      181 tttpydpcas npcihgacqs tdfydysctc eygyvgrnce nilkqcelll pcrnggtctd
      241 lhgsykcdcr lgyngqncek laeitydvtf rgdgwleldr svmtheeere vlgfeistnk
      301 tqglimwhgq tpndlnpdhy mslavvdgyv eyqynlgtgp vvirvtaqkv ddgerhriil
      361 krqgsdgsie lngehmesgs sygtqqelnt rgsvylggvp dyamtygkyq egfsgciytm
      421 evqdsgaidi gekairgknv spctrarwip sslvftnadt difdafvppp pvniihpkpa
      481 vnlaaynikh ysllillqnl lafamdireq svlftvlcid ivnaikcils
//
LOCUS       XP_034185492             303 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase PTC7 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034185492
VERSION     XP_034185492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="protein phosphatase PTC7 homolog"
                     /calculated_mol_wt=32913
     Region          62..298
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cl00120"
                     /db_xref="CDD:469621"
     CDS             1..303
                     /gene="LOC117606743"
                     /coded_by="XM_034329601.2:307..1218"
                     /db_xref="GeneID:117606743"
ORIGIN      
        1 mqsiywtgrl lsraiwngis nytacadpnl nnrreasfis avcgfpkdfa rgrlrkgqfg
       61 ddawfsakfk tvevigvadg vggwrhygid pgefssflmr tcerlvtmgr ftpseparll
      121 ansyyellen kqpilgssta cviilnkets siyaanigds gfvvvrkgev vhrsseqqhy
      181 fntpfqlslp ppghsglvls dspesadtss fgvedgdvil latdgvfdnv pdqilitemr
      241 kvqgerdptk iqgvansiaw marslafdga fmspfaqsar engidtiggk pdditvllat
      301 vai
//
LOCUS       XP_034185311             286 aa            linear   INV 03-SEP-2025
DEFINITION  isoprenylcysteine carboxylmethyltransferase ste14 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185311
VERSION     XP_034185311.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329420.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185311.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="isoprenylcysteine carboxylmethyltransferase
                     ste14"
                     /calculated_mol_wt=32592
     Region          165..257
                     /region_name="ICMT"
                     /note="Isoprenylcysteine carboxyl methyltransferase (ICMT)
                     family; cl21511"
                     /db_xref="CDD:473892"
     CDS             1..286
                     /gene="ste14"
                     /coded_by="XM_034329420.2:301..1161"
                     /db_xref="GeneID:117606666"
ORIGIN      
        1 mlsydgklsl fcftaaavia vlpeillrle fdipdayfsn iwlthlfqyg vlnlllilaf
       61 rgfsyqvavr aaflgyvfgt giivafvapp swqmfgvyit vlatfhytef lsiawinpst
      121 lsidsfilnd svaygvaacs gwmefiterh yfptmkessf isylglilci ggeilrksam
      181 ftakhnfnhi vqsekmenhe lvtcgvyrlc rhpsyvgwfy wsigtqlilq npfcllayav
      241 aswkffhdri lfeeitllnf fgeeyieyqk rvgtglpfis gykvnl
//
LOCUS       XP_034185493             238 aa            linear   INV 03-SEP-2025
DEFINITION  derlin 2 [Osmia lignaria lignaria].
ACCESSION   XP_034185493
VERSION     XP_034185493.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329602.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..238
                     /product="derlin 2"
                     /calculated_mol_wt=27910
     Region          13..203
                     /region_name="DER1"
                     /note="Der1-like family; pfam04511"
                     /db_xref="CDD:427988"
     CDS             1..238
                     /gene="Der-2"
                     /coded_by="XM_034329602.2:190..906"
                     /db_xref="GeneID:117606744"
ORIGIN      
        1 mayqtfrqey mqipvvtray ttacvittla vqldlvspfr lyfnptliie qyqlwrlitt
       61 flffgnmgfn flfnmiftyr ycrmleegsf rrrtsdfvmm fifggicmit faffvnllfl
      121 ghaftimlvy vwsrrnpfvr lnffgllnfh apylpwvllg fsvllgntiw vdlvgmavgh
      181 myyfaedvfp rlrggfrvlk tpqilktlfd ahpedpdytp ppeerpggfn wgqepnve
//
LOCUS       XP_034185498             127 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase 1 regulatory subunit 14B [Osmia lignaria
            lignaria].
ACCESSION   XP_034185498
VERSION     XP_034185498.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329607.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..127
                     /product="protein phosphatase 1 regulatory subunit 14B"
                     /calculated_mol_wt=14727
     Region          4..127
                     /region_name="PP1_inhibitor"
                     /note="PKC-activated protein phosphatase-1 inhibitor;
                     pfam05361"
                     /db_xref="CDD:461630"
     CDS             1..127
                     /gene="LOC117606745"
                     /coded_by="XM_034329607.2:157..540"
                     /db_xref="GeneID:117606745"
ORIGIN      
        1 mmegggvlta ersparanlh vaftekgevk errekfltak ygshqmslir krlavemwlf
       61 deleklyesv nesgknreve ididelldmd sdehrrrflq ellvdtkkpp hdinkfindl
      121 lekaktl
//
LOCUS       XP_034185242            2568 aa            linear   INV 03-SEP-2025
DEFINITION  calcineurin-binding protein cabin-1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185242
VERSION     XP_034185242.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329351.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185242.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2568
                     /product="calcineurin-binding protein cabin-1 isoform X1"
                     /calculated_mol_wt=291175
     Region          36..240
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          85..113
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(86,89..90,93..94,96,120,123..124,127..128,130..131,
                     154,157..158,161..162,165)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          118..148
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          153..181
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          187..211
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <303..481
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          2001..2409
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          2233..>2548
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..2568
                     /gene="LOC117606639"
                     /coded_by="XM_034329351.2:138..7844"
                     /db_xref="GeneID:117606639"
ORIGIN      
        1 mikisalnee sseeseeedv ptitkeaqeq ialteynkal ellkenkqed alnifkdlle
       61 telldevekp eipdgrsrpm lslkyscfkn igaiqatlen ydaaienywe aanldgtdvt
      121 lwyrigmlam kisnlelacs sfkqglkcns nhwpcldnmi talyavpdym ncllyismal
      181 erdpnyvkgl afrekifkdi pcleecykly nsdwqldppl yteydrvige klireaqeva
      241 gkwveackae ftakplpelt lrkpiksytw vdlgeclldm hkyitendls fvsrinlavq
      301 nfeespksna egdiqennnv ntdtevsqsa vvkkendvdk lirmdidean spfeisddnq
      361 vyggtdtmmd idpeddkkss ssdvqiiedd dplrisdtda aeeqnqlknd leeqgnfeed
      421 engdkmmdda ynsenriqnd nasdkdsnks ndkeyekdsk ktdkdqckgd eksndkieek
      481 dkvqkvkkrr rsalcflqqw awscnsmrrs arvrssnrre aerddvqlee tlrrifpstl
      541 lpdtvkltkd dpsknmddsm dtmelyqlfa nqennsniae tfkssdssks pspdtsrqqh
      601 yfgteseaad vnlfinqhsg ksnlmiiiar yteflctkwn qewpkgmsdv ylqaytfmre
      661 hiphpssfee didddvlkld temtllfgel htdrwlnnkp dilpsstldk lgtglpseel
      721 ghiiftsvrr dilneenmfi llrvlwlkan vflcqgdtdi vidslellsk hmkelekqnq
      781 nvylklpnck hncqisikiv ekklksiqrg qklgeiqhly dekkyaelay vlqdtfkfak
      841 qgnkllsvnd nivdrvqqls mlldslwqlq qyeecyvwae aclneswqny lntadeaeqk
      901 kwtssvlmal eklesctiev stfvvkylpe trlsrlvqnl vhivchqldv settvemple
      961 tvlpwillhy vlqykedker akaessyknk hssnysesdd edegvpasit ilfiahnfig
     1021 rhswccynea kllfftlslv ipkleapqfs sikgkvskyl eqifyclygh cnkvnkgrpk
     1081 hiedhgvpqm kmtwegaqll fdfykpkqip efysprslti sadteelfkr iiklippesn
     1141 pnqvidemma yimgerktmv tvkkplphsm stiyyllgdf yfknckwsaa iryylldlcf
     1201 hpsrlsswtg lamssgtvie twlnrykpin edkllakakm tqssyqhave ldscnhiiwt
     1261 eygtfayvvh sfcsrllkqe tdtlsmerfe vletrkeeml eiahlcfqsc ahlfigdkdg
     1321 viphderwly qymlgkiaek knqdppifle hyakaselly hnhaqyprri shkgpqhlsv
     1381 ealevhyrih asvlkyleqh egkplkkslg qvlqlhleks aqgpfmehns kvnekskedn
     1441 lfgsnkgsgk emevsgeter nvvaisqrsn sieeieivdg ttkgfshrve qmktetrkrs
     1501 pselpqddvk kiklgnvshl qlmqdvvali ddvitkvcdm vlqkeksddi mvlssdesne
     1561 sksqkkkakr nvektksrak sdeskkssmk mnnvfeteks envqdlmdal mkqameisqe
     1621 trqstpeded trkfdgkwlq sedlqltskd skekpsdkkk llkeemtisr rgsqestttt
     1681 qtttttetnn ssssssdess ssddssdsds ssdtdsdsve sdnekkkket nfaqkeetmt
     1741 eeevatliay clagleqcil rfsehyksfy rlshfffnnk takditkcrn llldtyncqf
     1801 yrgenfqglf sdrkstnffs gvwhipnrev drpgsfafhm srcvtllmqv lketndgrml
     1861 mqlsvqlgki pesdkkylrd sereqlscqa ltlclqslrt kvqlmgasts dapsnkntda
     1921 rtqvlldtyw vyqralksyh tkeqtiqtla sllvdtykty ignknlegnv leiatkycnd
     1981 yncnrkllnk ffcnldkplp dtqpqpqats pvpviasqpq vtstspqssq nrkpyrsglt
     2041 ttgrprgrpp nvnkylptmq hgnpmnqfnp kanfpnymga sgnrslmnpy fmhplvdpni
     2101 lsallstgls ssmmdplsam nylnqmgnyq difrqytnnl sslsnltsgl ssaiatapni
     2161 snvstmstst snmnaapnms lnnlnnltvq qllslsnsta ttvrsspmyq qaataitttt
     2221 asitkdrpsi sitpvntapa kskpskqspq seplpvqlpk slqisppskp vlqppttqvs
     2281 llkpsilqhv ktsppkqmsa pqirvskslt epqpahnpsl shsplksntg tnpttvpqva
     2341 hstmgpsmtm kqtlpmnvps sgtslqhkll skknsqrsya qasvqnpark tkpktmpals
     2401 gnfsnmlnai pgnssmpqpp yippelsgis vsavnpqtgl kgpaakysgy kkpsvktkpt
     2461 lptdaaslpt aysqgnsaea lsmlsqlkqh shleiipqqk thmkpsmeyt kslssgvtvv
     2521 pqkvsetlrs ntdcmtiydl prvksssvtg kkgekgands veiitldd
//
LOCUS       XP_034185244            2564 aa            linear   INV 03-SEP-2025
DEFINITION  calcineurin-binding protein cabin-1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185244
VERSION     XP_034185244.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329353.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185244.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2564
                     /product="calcineurin-binding protein cabin-1 isoform X2"
                     /calculated_mol_wt=290605
     Region          36..240
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          85..113
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(86,89..90,93..94,96,120,123..124,127..128,130..131,
                     154,157..158,161..162,165)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          118..148
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          153..181
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          187..211
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <303..481
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          1997..2405
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          2229..>2544
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..2564
                     /gene="LOC117606639"
                     /coded_by="XM_034329353.2:138..7832"
                     /db_xref="GeneID:117606639"
ORIGIN      
        1 mikisalnee sseeseeedv ptitkeaqeq ialteynkal ellkenkqed alnifkdlle
       61 telldevekp eipdgrsrpm lslkyscfkn igaiqatlen ydaaienywe aanldgtdvt
      121 lwyrigmlam kisnlelacs sfkqglkcns nhwpcldnmi talyavpdym ncllyismal
      181 erdpnyvkgl afrekifkdi pcleecykly nsdwqldppl yteydrvige klireaqeva
      241 gkwveackae ftakplpelt lrkpiksytw vdlgeclldm hkyitendls fvsrinlavq
      301 nfeespksna egdiqennnv ntdtevsqsa vvkkendvdk lirmdidean spfeisddnq
      361 vyggtdtmmd idpeddkkss ssdvqiiedd dplrisdtda aeeqnqlknd leeqgnfeed
      421 engdkmmdda ynsenriqnd nasdkdsnks ndkeyekdsk ktdkdqckgd eksndkieek
      481 dkvqkvkkrr rsalcflqqw awscnsmrrs arvrssnrre aerddvqlee tlrrifpstl
      541 lpdtvkltkd dpsknmddsm dtmelyqlfa nqennsniae tfkssdssks pspdtsrqqh
      601 yfgteseaad vnlfinqhsg ksnlmiiiar yteflctkwn qewpkgmsdv ylqaytfmre
      661 hiphpssfee didddvlkld temtllfgel htdrwlnnkp dilpsstldk lgtglpseel
      721 ghiiftsvrr dilneenmfi llrvlwlkan vflcqgdtdi vidslellsk hmkelekqnq
      781 nvylklpnck hncqisikiv ekklksiqrg qklgeiqhly dekkyaelay vlqdtfkfak
      841 qgnkllsvnd nivdrvqqls mlldslwqlq qyeecyvwae aclneswqny lntadeaeqk
      901 kwtssvlmal eklesctiev stfvvkylpe trlsrlvqnl vhivchqldv settvemple
      961 tvlpwillhy vlqykedker akaessyknk hssnysesdd edegvpasit ilfiahnfig
     1021 rhswccynea kllfftlslv ipkleapqfs sikgkvskyl eqifyclygh cnkvnkgrpk
     1081 hiedhgvpqm kmtwegaqll fdfykpkqip efysprslti sadteelfkr iiklippesn
     1141 pnqvidemma yimgerktmv tvkkplphsm stiyyllgdf yfknckwsaa iryylldlcf
     1201 hpsrlsswtg lamssgtvie twlnsnedkl lakakmtqss yqhaveldsc nhiiwteygt
     1261 fayvvhsfcs rllkqetdtl smerfevlet rkeemleiah lcfqscahlf igdkdgviph
     1321 derwlyqyml gkiaekknqd ppiflehyak asellyhnha qyprrishkg pqhlsveale
     1381 vhyrihasvl kyleqhegkp lkkslgqvlq lhleksaqgp fmehnskvne kskednlfgs
     1441 nkgsgkemev sgeternvva isqrsnsiee ieivdgttkg fshrveqmkt etrkrspsel
     1501 pqddvkkikl gnvshlqlmq dvvaliddvi tkvcdmvlqk eksddimvls sdesnesksq
     1561 kkkakrnvek tksraksdes kkssmkmnnv feteksenvq dlmdalmkqa meisqetrqs
     1621 tpededtrkf dgkwlqsedl qltskdskek psdkkkllke emtisrrgsq esttttqttt
     1681 ttetnnssss ssdessssdd ssdsdsssdt dsdsvesdne kkkketnfaq keetmteeev
     1741 atliayclag leqcilrfse hyksfyrlsh fffnnktakd itkcrnllld tyncqfyrge
     1801 nfqglfsdrk stnffsgvwh ipnrevdrpg sfafhmsrcv tllmqvlket ndgrmlmqls
     1861 vqlgkipesd kkylrdsere qlscqaltlc lqslrtkvql mgastsdaps nkntdartqv
     1921 lldtywvyqr alksyhtkeq tiqtlasllv dtyktyignk nlegnvleia tkycndyncn
     1981 rkllnkffcn ldkplpdtqp qpqatspvpv iasqpqvtst spqssqnrkp yrsgltttgr
     2041 prgrppnvnk ylptmqhgnp mnqfnpkanf pnymgasgnr slmnpyfmhp lvdpnilsal
     2101 lstglsssmm dplsamnyln qmgnyqdifr qytnnlssls nltsglssai atapnisnvs
     2161 tmststsnmn aapnmslnnl nnltvqqlls lsnstattvr sspmyqqaat aittttasit
     2221 kdrpsisitp vntapakskp skqspqsepl pvqlpkslqi sppskpvlqp pttqvsllkp
     2281 silqhvktsp pkqmsapqir vsksltepqp ahnpslshsp lksntgtnpt tvpqvahstm
     2341 gpsmtmkqtl pmnvpssgts lqhkllskkn sqrsyaqasv qnparktkpk tmpalsgnfs
     2401 nmlnaipgns smpqppyipp elsgisvsav npqtglkgpa akysgykkps vktkptlptd
     2461 aaslptaysq gnsaealsml sqlkqhshle iipqqkthmk psmeytksls sgvtvvpqkv
     2521 setlrsntdc mtiydlprvk sssvtgkkge kgandsveii tldd
//
LOCUS       XP_034185229            2549 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein upSET isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185229
VERSION     XP_034185229.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185229.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2549
                     /product="SET domain-containing protein upSET isoform X1"
                     /calculated_mol_wt=280095
     Region          868..911
                     /region_name="PHD_MLL5"
                     /note="PHD finger found in mixed lineage leukemia 5
                     (MLL5); cd15550"
                     /db_xref="CDD:277025"
     Site            order(876,878..882,884,889,902..906)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:277025"
     Region          1103..1216
                     /region_name="SET_SETD5-like"
                     /note="SET domain found in SET domain-containing protein 5
                     (SETD5), inactive histone-lysine N-methyltransferase 2E
                     (KMT2E) and similar proteins; cd10529"
                     /db_xref="CDD:380927"
     Site            order(1104..1105,1122,1132,1146..1153,1174..1178,1186,
                     1202,1213..1216)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380927"
     Site            order(1104..1105,1146..1149,1174..1178,1215)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380927"
     Region          1501..>1747
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          <2091..>2297
                     /region_name="rad2"
                     /note="DNA excision repair protein (rad2); TIGR00600"
                     /db_xref="CDD:273166"
     CDS             1..2549
                     /gene="upSET"
                     /coded_by="XM_034329338.2:570..8219"
                     /db_xref="GeneID:117606635"
ORIGIN      
        1 msfvlqlgti easleknstn qpssslntds isnddagksp qilqclents eiptghvisf
       61 stvksvsvty pvaktvkevq rvagpqtntt qvlttrvisq klpssghqaq ptpmtlnasp
      121 nvahvpinaq pipspgngva hvyplqhptm ttqskqqarn qvmssdgkqq qqqvttmasl
      181 plkvqpvspq lvvsssavra intvtslpni qrihvkaqnl vgqpqtvnlq kvktvgnvsq
      241 gvtvqrnsvp riqtvqksqv ptatvptaqf avnqvtnnan vprsqqgnst lqkaqantvn
      301 aqkvaqvynn qkissqmlns spnhkaqlqq itgnqqqqgl qklqvqsqkt vavprqqqsa
      361 ttvvnnvqkp snsvagiqkv qvvgqvqcqq qagqhvqkhv qqvqsaqhqr lqtatvalqk
      421 sqtvaavnss rvqtitnvck snsvpniart pqnanllaas kqqaapsqpq qsqqghaqss
      481 aqlqqqmlqq ssqqqqpmqk qqqananqqv qrshgignvh qkaatiatmp ngqraqvnsk
      541 ipqqqmvmrv gvpksqgqnl qqsnlktvpq kisntvkssg sqnsvqhpmh rnansqpvki
      601 iqqqqqqqqq qqnvmgpqnt qkqpgcikti paqkpaqrnh aqkvtgikts lntnvnpakn
      661 qggattavaa kasiktllpq qtvasnmlmh knqpikiqqq tmqqkqlvaa sqfpqqvrqq
      721 sgqvktllpv ftgeprkdve nksegesres kederqprpa spiiripppy eclqyvlqdh
      781 nygappprtp sppsppshpk qapingacss ttasqhpfiy skvvtcpnvd ddaasaisse
      841 tgrddepege etetapegeg ddedsvtrci cdfehddgym iccdrclvwq hvdcmgidrs
      901 nipdeylcei crprrvdrqr aralqmrkre ellnsdtssd tsstssadtd vgvntipkkr
      961 tlqqqqvlrr kseppqvrrl nnnnnnnnnn vakrqrrdsh prqsssvrkk eatkrngpgk
     1021 rkakrrmsle dkeeetqdtw asnvaplrqw ieryeeavtn hyspelrari ssikvngths
     1081 dlrqsnmnvi atgkcrlnvh snnvrflvat mylppntpvv elrgkymlst qhrpsypqgr
     1141 qhtqrpgpfv ffyrlprdgt evcvdtrtyg ndarfvrrsc kpnaevkhci ekgtlhlyiv
     1201 tttaieknae itirheqhdl llspnpngpm mpivcacnnp recqivslnq lnrrgsngal
     1261 aenadgrerr rrgrrntvce dsdsstavps tvvtqpttps sttvsssvss vppkrtiatt
     1321 vanavrqapk eeapmtvqqp qtspnpsqpv assetkkdkk kmtreerkme aimkaferle
     1381 kaeqrkqevq arnaqrkesg gthsdnedah vslqskqkqq nserplrrkr rkgrarttss
     1441 sqsssrrtrl nsadsdessg destsmqspp lsqnhsqnrd apysaqlhtp vknanegvnt
     1501 sghqgiptaa glllalansn apgpsspplr qptpvksptc dsgassssqs stpstplssa
     1561 cllvaaavgp lapgfkfpkt kkvlmnewlk espdppqnnv sqvsplqalp paapvsnsvt
     1621 alcrstdfsl pgdssaefls qsyaakslat lvqaansvsg icdsppqrrq aaagntvcpv
     1681 stgsakkrwl rqaiseecds pnsrpesppp semvappkkr riareslssd nytppttptm
     1741 lvpesapnnr slcpteddfi eqphsplves seehqprteg vkqemldsdd hgseevrrkl
     1801 sidiprefnv ktvkkeeets tgrsepslvk sedvqvkeet keemecdsya sletkpfvkn
     1861 eyrsvknemi nrhrdkckre erikkeeeme iekgrveiid qnegdtemed lsspigames
     1921 dvilkqrvae mrlefgasis emmsaended rkseradeks ddnmsidefd veaqmkkitg
     1981 ddgndykekv dsssekdksm dgieglmess kedsesedke iddlkyetnf ksfhlnheek
     2041 lfnefendhh rqnnvvedsg akemeeqsee sqktehpsvs vasseesife stssnmdaes
     2101 vadppkifhs ipplserirk ktevasapkt hlnfeaaiie stidmdsvdg skngeqqksm
     2161 lstalrelle aklddltsdg akeettddns tpcpepeakt epqeqsveve dtvlktvsen
     2221 vhseeastke tenppkeikr lkdprtvvpn smpapafkpe tnppvkrkls iseyrkrkqq
     2281 stgtppepep ssdtsttdks gargrsdsas sgtsslssde egskismsld iptltalplf
     2341 tntesdekkg geegtigwsa aptlverqre nlterlkref glflsddeee rarkhgltae
     2401 ailkarkasp phptvsstpp gypvppqlpp qpyipppgsa sihyaqfqak pcpvqyqnft
     2461 vpqnvaqpiy anaapqattt kpaqqqflvp qasqappgsn pyppqfipps ttavpvakfs
     2521 svtpppgnqi ypasgqsqkq fynhpaprs
//
LOCUS       XP_034185230            2549 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein upSET isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185230
VERSION     XP_034185230.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185230.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2549
                     /product="SET domain-containing protein upSET isoform X1"
                     /calculated_mol_wt=280095
     Region          868..911
                     /region_name="PHD_MLL5"
                     /note="PHD finger found in mixed lineage leukemia 5
                     (MLL5); cd15550"
                     /db_xref="CDD:277025"
     Site            order(876,878..882,884,889,902..906)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:277025"
     Region          1103..1216
                     /region_name="SET_SETD5-like"
                     /note="SET domain found in SET domain-containing protein 5
                     (SETD5), inactive histone-lysine N-methyltransferase 2E
                     (KMT2E) and similar proteins; cd10529"
                     /db_xref="CDD:380927"
     Site            order(1104..1105,1122,1132,1146..1153,1174..1178,1186,
                     1202,1213..1216)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380927"
     Site            order(1104..1105,1146..1149,1174..1178,1215)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380927"
     Region          1501..>1747
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          <2091..>2297
                     /region_name="rad2"
                     /note="DNA excision repair protein (rad2); TIGR00600"
                     /db_xref="CDD:273166"
     CDS             1..2549
                     /gene="upSET"
                     /coded_by="XM_034329339.2:866..8515"
                     /db_xref="GeneID:117606635"
ORIGIN      
        1 msfvlqlgti easleknstn qpssslntds isnddagksp qilqclents eiptghvisf
       61 stvksvsvty pvaktvkevq rvagpqtntt qvlttrvisq klpssghqaq ptpmtlnasp
      121 nvahvpinaq pipspgngva hvyplqhptm ttqskqqarn qvmssdgkqq qqqvttmasl
      181 plkvqpvspq lvvsssavra intvtslpni qrihvkaqnl vgqpqtvnlq kvktvgnvsq
      241 gvtvqrnsvp riqtvqksqv ptatvptaqf avnqvtnnan vprsqqgnst lqkaqantvn
      301 aqkvaqvynn qkissqmlns spnhkaqlqq itgnqqqqgl qklqvqsqkt vavprqqqsa
      361 ttvvnnvqkp snsvagiqkv qvvgqvqcqq qagqhvqkhv qqvqsaqhqr lqtatvalqk
      421 sqtvaavnss rvqtitnvck snsvpniart pqnanllaas kqqaapsqpq qsqqghaqss
      481 aqlqqqmlqq ssqqqqpmqk qqqananqqv qrshgignvh qkaatiatmp ngqraqvnsk
      541 ipqqqmvmrv gvpksqgqnl qqsnlktvpq kisntvkssg sqnsvqhpmh rnansqpvki
      601 iqqqqqqqqq qqnvmgpqnt qkqpgcikti paqkpaqrnh aqkvtgikts lntnvnpakn
      661 qggattavaa kasiktllpq qtvasnmlmh knqpikiqqq tmqqkqlvaa sqfpqqvrqq
      721 sgqvktllpv ftgeprkdve nksegesres kederqprpa spiiripppy eclqyvlqdh
      781 nygappprtp sppsppshpk qapingacss ttasqhpfiy skvvtcpnvd ddaasaisse
      841 tgrddepege etetapegeg ddedsvtrci cdfehddgym iccdrclvwq hvdcmgidrs
      901 nipdeylcei crprrvdrqr aralqmrkre ellnsdtssd tsstssadtd vgvntipkkr
      961 tlqqqqvlrr kseppqvrrl nnnnnnnnnn vakrqrrdsh prqsssvrkk eatkrngpgk
     1021 rkakrrmsle dkeeetqdtw asnvaplrqw ieryeeavtn hyspelrari ssikvngths
     1081 dlrqsnmnvi atgkcrlnvh snnvrflvat mylppntpvv elrgkymlst qhrpsypqgr
     1141 qhtqrpgpfv ffyrlprdgt evcvdtrtyg ndarfvrrsc kpnaevkhci ekgtlhlyiv
     1201 tttaieknae itirheqhdl llspnpngpm mpivcacnnp recqivslnq lnrrgsngal
     1261 aenadgrerr rrgrrntvce dsdsstavps tvvtqpttps sttvsssvss vppkrtiatt
     1321 vanavrqapk eeapmtvqqp qtspnpsqpv assetkkdkk kmtreerkme aimkaferle
     1381 kaeqrkqevq arnaqrkesg gthsdnedah vslqskqkqq nserplrrkr rkgrarttss
     1441 sqsssrrtrl nsadsdessg destsmqspp lsqnhsqnrd apysaqlhtp vknanegvnt
     1501 sghqgiptaa glllalansn apgpsspplr qptpvksptc dsgassssqs stpstplssa
     1561 cllvaaavgp lapgfkfpkt kkvlmnewlk espdppqnnv sqvsplqalp paapvsnsvt
     1621 alcrstdfsl pgdssaefls qsyaakslat lvqaansvsg icdsppqrrq aaagntvcpv
     1681 stgsakkrwl rqaiseecds pnsrpesppp semvappkkr riareslssd nytppttptm
     1741 lvpesapnnr slcpteddfi eqphsplves seehqprteg vkqemldsdd hgseevrrkl
     1801 sidiprefnv ktvkkeeets tgrsepslvk sedvqvkeet keemecdsya sletkpfvkn
     1861 eyrsvknemi nrhrdkckre erikkeeeme iekgrveiid qnegdtemed lsspigames
     1921 dvilkqrvae mrlefgasis emmsaended rkseradeks ddnmsidefd veaqmkkitg
     1981 ddgndykekv dsssekdksm dgieglmess kedsesedke iddlkyetnf ksfhlnheek
     2041 lfnefendhh rqnnvvedsg akemeeqsee sqktehpsvs vasseesife stssnmdaes
     2101 vadppkifhs ipplserirk ktevasapkt hlnfeaaiie stidmdsvdg skngeqqksm
     2161 lstalrelle aklddltsdg akeettddns tpcpepeakt epqeqsveve dtvlktvsen
     2221 vhseeastke tenppkeikr lkdprtvvpn smpapafkpe tnppvkrkls iseyrkrkqq
     2281 stgtppepep ssdtsttdks gargrsdsas sgtsslssde egskismsld iptltalplf
     2341 tntesdekkg geegtigwsa aptlverqre nlterlkref glflsddeee rarkhgltae
     2401 ailkarkasp phptvsstpp gypvppqlpp qpyipppgsa sihyaqfqak pcpvqyqnft
     2461 vpqnvaqpiy anaapqattt kpaqqqflvp qasqappgsn pyppqfipps ttavpvakfs
     2521 svtpppgnqi ypasgqsqkq fynhpaprs
//
LOCUS       XP_034185231            2549 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein upSET isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185231
VERSION     XP_034185231.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329340.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185231.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2549
                     /product="SET domain-containing protein upSET isoform X1"
                     /calculated_mol_wt=280095
     Region          868..911
                     /region_name="PHD_MLL5"
                     /note="PHD finger found in mixed lineage leukemia 5
                     (MLL5); cd15550"
                     /db_xref="CDD:277025"
     Site            order(876,878..882,884,889,902..906)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:277025"
     Region          1103..1216
                     /region_name="SET_SETD5-like"
                     /note="SET domain found in SET domain-containing protein 5
                     (SETD5), inactive histone-lysine N-methyltransferase 2E
                     (KMT2E) and similar proteins; cd10529"
                     /db_xref="CDD:380927"
     Site            order(1104..1105,1122,1132,1146..1153,1174..1178,1186,
                     1202,1213..1216)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380927"
     Site            order(1104..1105,1146..1149,1174..1178,1215)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380927"
     Region          1501..>1747
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          <2091..>2297
                     /region_name="rad2"
                     /note="DNA excision repair protein (rad2); TIGR00600"
                     /db_xref="CDD:273166"
     CDS             1..2549
                     /gene="upSET"
                     /coded_by="XM_034329340.2:699..8348"
                     /db_xref="GeneID:117606635"
ORIGIN      
        1 msfvlqlgti easleknstn qpssslntds isnddagksp qilqclents eiptghvisf
       61 stvksvsvty pvaktvkevq rvagpqtntt qvlttrvisq klpssghqaq ptpmtlnasp
      121 nvahvpinaq pipspgngva hvyplqhptm ttqskqqarn qvmssdgkqq qqqvttmasl
      181 plkvqpvspq lvvsssavra intvtslpni qrihvkaqnl vgqpqtvnlq kvktvgnvsq
      241 gvtvqrnsvp riqtvqksqv ptatvptaqf avnqvtnnan vprsqqgnst lqkaqantvn
      301 aqkvaqvynn qkissqmlns spnhkaqlqq itgnqqqqgl qklqvqsqkt vavprqqqsa
      361 ttvvnnvqkp snsvagiqkv qvvgqvqcqq qagqhvqkhv qqvqsaqhqr lqtatvalqk
      421 sqtvaavnss rvqtitnvck snsvpniart pqnanllaas kqqaapsqpq qsqqghaqss
      481 aqlqqqmlqq ssqqqqpmqk qqqananqqv qrshgignvh qkaatiatmp ngqraqvnsk
      541 ipqqqmvmrv gvpksqgqnl qqsnlktvpq kisntvkssg sqnsvqhpmh rnansqpvki
      601 iqqqqqqqqq qqnvmgpqnt qkqpgcikti paqkpaqrnh aqkvtgikts lntnvnpakn
      661 qggattavaa kasiktllpq qtvasnmlmh knqpikiqqq tmqqkqlvaa sqfpqqvrqq
      721 sgqvktllpv ftgeprkdve nksegesres kederqprpa spiiripppy eclqyvlqdh
      781 nygappprtp sppsppshpk qapingacss ttasqhpfiy skvvtcpnvd ddaasaisse
      841 tgrddepege etetapegeg ddedsvtrci cdfehddgym iccdrclvwq hvdcmgidrs
      901 nipdeylcei crprrvdrqr aralqmrkre ellnsdtssd tsstssadtd vgvntipkkr
      961 tlqqqqvlrr kseppqvrrl nnnnnnnnnn vakrqrrdsh prqsssvrkk eatkrngpgk
     1021 rkakrrmsle dkeeetqdtw asnvaplrqw ieryeeavtn hyspelrari ssikvngths
     1081 dlrqsnmnvi atgkcrlnvh snnvrflvat mylppntpvv elrgkymlst qhrpsypqgr
     1141 qhtqrpgpfv ffyrlprdgt evcvdtrtyg ndarfvrrsc kpnaevkhci ekgtlhlyiv
     1201 tttaieknae itirheqhdl llspnpngpm mpivcacnnp recqivslnq lnrrgsngal
     1261 aenadgrerr rrgrrntvce dsdsstavps tvvtqpttps sttvsssvss vppkrtiatt
     1321 vanavrqapk eeapmtvqqp qtspnpsqpv assetkkdkk kmtreerkme aimkaferle
     1381 kaeqrkqevq arnaqrkesg gthsdnedah vslqskqkqq nserplrrkr rkgrarttss
     1441 sqsssrrtrl nsadsdessg destsmqspp lsqnhsqnrd apysaqlhtp vknanegvnt
     1501 sghqgiptaa glllalansn apgpsspplr qptpvksptc dsgassssqs stpstplssa
     1561 cllvaaavgp lapgfkfpkt kkvlmnewlk espdppqnnv sqvsplqalp paapvsnsvt
     1621 alcrstdfsl pgdssaefls qsyaakslat lvqaansvsg icdsppqrrq aaagntvcpv
     1681 stgsakkrwl rqaiseecds pnsrpesppp semvappkkr riareslssd nytppttptm
     1741 lvpesapnnr slcpteddfi eqphsplves seehqprteg vkqemldsdd hgseevrrkl
     1801 sidiprefnv ktvkkeeets tgrsepslvk sedvqvkeet keemecdsya sletkpfvkn
     1861 eyrsvknemi nrhrdkckre erikkeeeme iekgrveiid qnegdtemed lsspigames
     1921 dvilkqrvae mrlefgasis emmsaended rkseradeks ddnmsidefd veaqmkkitg
     1981 ddgndykekv dsssekdksm dgieglmess kedsesedke iddlkyetnf ksfhlnheek
     2041 lfnefendhh rqnnvvedsg akemeeqsee sqktehpsvs vasseesife stssnmdaes
     2101 vadppkifhs ipplserirk ktevasapkt hlnfeaaiie stidmdsvdg skngeqqksm
     2161 lstalrelle aklddltsdg akeettddns tpcpepeakt epqeqsveve dtvlktvsen
     2221 vhseeastke tenppkeikr lkdprtvvpn smpapafkpe tnppvkrkls iseyrkrkqq
     2281 stgtppepep ssdtsttdks gargrsdsas sgtsslssde egskismsld iptltalplf
     2341 tntesdekkg geegtigwsa aptlverqre nlterlkref glflsddeee rarkhgltae
     2401 ailkarkasp phptvsstpp gypvppqlpp qpyipppgsa sihyaqfqak pcpvqyqnft
     2461 vpqnvaqpiy anaapqattt kpaqqqflvp qasqappgsn pyppqfipps ttavpvakfs
     2521 svtpppgnqi ypasgqsqkq fynhpaprs
//
LOCUS       XP_034185232            2542 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein upSET isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185232
VERSION     XP_034185232.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329341.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185232.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2542
                     /product="SET domain-containing protein upSET isoform X2"
                     /calculated_mol_wt=279247
     Region          861..904
                     /region_name="PHD_MLL5"
                     /note="PHD finger found in mixed lineage leukemia 5
                     (MLL5); cd15550"
                     /db_xref="CDD:277025"
     Site            order(869,871..875,877,882,895..899)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:277025"
     Region          1096..1209
                     /region_name="SET_SETD5-like"
                     /note="SET domain found in SET domain-containing protein 5
                     (SETD5), inactive histone-lysine N-methyltransferase 2E
                     (KMT2E) and similar proteins; cd10529"
                     /db_xref="CDD:380927"
     Site            order(1097..1098,1115,1125,1139..1146,1167..1171,1179,
                     1195,1206..1209)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380927"
     Site            order(1097..1098,1139..1142,1167..1171,1208)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380927"
     Region          1494..>1740
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     Region          <2084..>2290
                     /region_name="rad2"
                     /note="DNA excision repair protein (rad2); TIGR00600"
                     /db_xref="CDD:273166"
     CDS             1..2542
                     /gene="upSET"
                     /coded_by="XM_034329341.2:570..8198"
                     /db_xref="GeneID:117606635"
ORIGIN      
        1 msfvlqlgti easleknstn qpssslntds isnddagksp qilqclents eiptghvisf
       61 stvksvsvty pvaktvkevq rvagpqtntt qvlttrvisq klpssghqaq ptpmtlnasp
      121 nvahvpinaq pipspgngva hvyplqhptm ttqskqqarn qvmssdgkqq qqqvttmasl
      181 plkvqpvspq lvvsssavra intvtslpni qrihvkaqnl vgqpqtvnlq kvktvgnvsq
      241 gvtvqrnsvp riqtvqksqv ptatvptaqf avnqvtnnan vprsqqgnst lqkaqantvn
      301 aqkvaqvynn qkissqmlns spnhkaqlqq itgnqqqqgl qklqvqsqkt vavprqqqsa
      361 ttvvnnvqkp snsvagiqkv qvvgqvqcqq qagqhvqkhv qqvqsaqhqr lqtatvalqk
      421 sqtvaavnss rvqtitnvck snsvpniart pqnanllaas kqqaapsqpq qsqqghaqss
      481 aqlqqqmlqq ssqqqqpmqk qqqananqqv qrshgignvh qkaatiatmp ngqraqvnsk
      541 ipqqqmvmrv gvpksqgqnl qqsnlktvpq kisntvkssg sqnsvqhpmh rnansqpvki
      601 iqqqqqqqqq qqnvmgpqnt qkqpgcikti paqkpaqrnh aqkvtgikts lntnvnpakn
      661 qggattavaa kasiktllpq qtvasnmlmh knqpikiqqq tmqqkqlvaa sqfpqqvrqq
      721 sgqvktllpv ftgeprkdve nksegesres kederqprpa spiiripppy edhnygappp
      781 rtpsppspps hpkqapinga cssttasqhp fiyskvvtcp nvdddaasai ssetgrddep
      841 egeetetape gegddedsvt rcicdfehdd gymiccdrcl vwqhvdcmgi drsnipdeyl
      901 ceicrprrvd rqraralqmr kreellnsdt ssdtsstssa dtdvgvntip kkrtlqqqqv
      961 lrrkseppqv rrlnnnnnnn nnnvakrqrr dshprqsssv rkkeatkrng pgkrkakrrm
     1021 sledkeeetq dtwasnvapl rqwieryeea vtnhyspelr arissikvng thsdlrqsnm
     1081 nviatgkcrl nvhsnnvrfl vatmylppnt pvvelrgkym lstqhrpsyp qgrqhtqrpg
     1141 pfvffyrlpr dgtevcvdtr tygndarfvr rsckpnaevk hciekgtlhl yivtttaiek
     1201 naeitirheq hdlllspnpn gpmmpivcac nnprecqivs lnqlnrrgsn galaenadgr
     1261 errrrgrrnt vcedsdssta vpstvvtqpt tpssttvsss vssvppkrti attvanavrq
     1321 apkeeapmtv qqpqtspnps qpvassetkk dkkkmtreer kmeaimkafe rlekaeqrkq
     1381 evqarnaqrk esggthsdne dahvslqskq kqqnserplr rkrrkgrart tsssqsssrr
     1441 trlnsadsde ssgdestsmq spplsqnhsq nrdapysaql htpvknaneg vntsghqgip
     1501 taaglllala nsnapgpssp plrqptpvks ptcdsgasss sqsstpstpl ssacllvaaa
     1561 vgplapgfkf pktkkvlmne wlkespdppq nnvsqvsplq alppaapvsn svtalcrstd
     1621 fslpgdssae flsqsyaaks latlvqaans vsgicdsppq rrqaaagntv cpvstgsakk
     1681 rwlrqaisee cdspnsrpes pppsemvapp kkrriaresl ssdnytpptt ptmlvpesap
     1741 nnrslcpted dfieqphspl vesseehqpr tegvkqemld sddhgseevr rklsidipre
     1801 fnvktvkkee etstgrseps lvksedvqvk eetkeemecd syasletkpf vkneyrsvkn
     1861 eminrhrdkc kreerikkee emeiekgrve iidqnegdte medlsspiga mesdvilkqr
     1921 vaemrlefga sisemmsaen dedrkserad eksddnmsid efdveaqmkk itgddgndyk
     1981 ekvdsssekd ksmdgieglm esskedsese dkeiddlkye tnfksfhlnh eeklfnefen
     2041 dhhrqnnvve dsgakemeeq seesqktehp svsvassees ifestssnmd aesvadppki
     2101 fhsipplser irkktevasa pkthlnfeaa iiestidmds vdgskngeqq ksmlstalre
     2161 lleaklddlt sdgakeettd dnstpcpepe aktepqeqsv evedtvlktv senvhseeas
     2221 tketenppke ikrlkdprtv vpnsmpapaf kpetnppvkr klsiseyrkr kqqstgtppe
     2281 pepssdtstt dksgargrsd sassgtssls sdeegskism sldiptltal plftntesde
     2341 kkggeegtig wsaaptlver qrenlterlk refglflsdd eeerarkhgl taeailkark
     2401 aspphptvss tppgypvppq lppqpyippp gsasihyaqf qakpcpvqyq nftvpqnvaq
     2461 piyanaapqa tttkpaqqqf lvpqasqapp gsnpyppqfi ppsttavpva kfssvtpppg
     2521 nqiypasgqs qkqfynhpap rs
//
LOCUS       XP_034185245            1412 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear pore complex protein Nup160 [Osmia lignaria lignaria].
ACCESSION   XP_034185245
VERSION     XP_034185245.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329354.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1412
                     /product="nuclear pore complex protein Nup160"
                     /calculated_mol_wt=160829
     Region          33..542
                     /region_name="Nup160"
                     /note="Nucleoporin Nup120/160; pfam11715"
                     /db_xref="CDD:432020"
     CDS             1..1412
                     /gene="Nup160"
                     /coded_by="XM_034329354.2:130..4368"
                     /db_xref="GeneID:117606640"
ORIGIN      
        1 meefplgyre vvpdqtvpek wnevtlntgg sqstlqdikv peraggysyn dcskyftrnr
       61 fiywhichdv leliehsldi nlancrvryk ftdtplldgi sihetinsvi ilivtvssvh
      121 klsfphpeti hknehslsty kdlgiqsifs eassqnakri kedphmfhvi tsagttnfpv
      181 phaaaswfip pqealfalay nsgtilllrl dtktglvhts elkqdsivpr flsgiatafg
      241 grsseahvam sltihscgnd tylfalcreg nirmwscnka qcvavtdaai dhriasqgaq
      301 ghmlrktlgp nnelylgifl kfstrcefsv lkpiqdngif kfirlctrfa qdqhlvdfsf
      361 tttrlwavwr ttdmdtvevt haqlslsgtr vnsewetail eqtpdadyvl gdpdtdprqa
      421 yidyifqpgq fsltditkal niyrrsncie ekilsvselk ervciaveae iqaeimdyel
      481 mdedyleian rcwskfyscv iqyhanstrp vgllllpnvy gvillkkssf sllrpmeale
      541 ylmfcneksy tsrfkttpvl sqdddicqdl itlmsalvli eeqlseetka amkrelyhlk
      601 spdiiiddll srlmleseep isdfithhps chrlsnirdi ssamamllea ltydlgqpnk
      661 lemngdhdas kvllninhfl ssqlgisaia ksvaqitllr fsicrnllil qqiillrpel
      721 fdsrslhsvk sslaprtvvl tqayyvvtwi cestalsapv qslldssiqr lamlkfvdar
      781 tnigfrqhrs msllelfiqn sgakhahslm ahtnlnantl ipwhygmlth vtliaqliwp
      841 rsgnfifpew llsscqfllv qeyvrllntw cewnsasrkf ilavallemg evqkacdhfl
      901 rasggiltde fladalldss emsessaliq yylkvvklfe ernaadcive laetaimmad
      961 kddpnlptlh sivftqhlnl ghhtkayncl nsnpdaarrv dclrqlvvtl ferkklidli
     1021 sfpyvdmyed lerivegrar svdlmennyy nflysfhink gnmrkaasvm yeqamrlsqe
     1081 sqsvpvvsrq aqcllacina lhlvsenyrw ivrpvidqny seemddqdiq srrsmsdkei
     1141 lnynikkqie vlelndikke yyivdarlkv skinselqvv thagpselvi ilstiglytt
     1201 alhlcdefki nkssvfeslt sqcirlsrre dsnawdwliq ndifdigltn tnltntawgl
     1261 lqyltlshek drsselhkav srkllqegaf ipqwllmsyk krnaaellrl mlntgrilea
     1321 sdlaveyiha vmgsgkiehf glqsfltard ppvwlpfntl eillyelkea seensifseg
     1381 yqklqealni ykekvirvsq dmiqlkfssk kh
//
LOCUS       XP_034185247            1361 aa            linear   INV 03-SEP-2025
DEFINITION  mitogen-activated protein kinase kinase kinase 4 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185247
VERSION     XP_034185247.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329356.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1361
                     /product="mitogen-activated protein kinase kinase kinase 4
                     isoform X1"
                     /calculated_mol_wt=155929
     Region          28..>807
                     /region_name="MEKK4_N"
                     /note="MEKK4 N-terminal; pfam19431"
                     /db_xref="CDD:466077"
     Region          1083..1344
                     /region_name="STKc_MEKK4"
                     /note="Catalytic domain of the Protein Serine/Threonine
                     Kinase, Mitogen-Activated Protein (MAP)/Extracellular
                     signal-Regulated Kinase (ERK) Kinase Kinase 4; cd06626"
                     /db_xref="CDD:270796"
     Site            order(1089..1093,1097,1110,1112,1143,1159..1162,
                     1165..1166,1169,1206,1208..1211,1213,1224..1225,1228,
                     1245..1248,1250,1280,1289)
                     /site_type="active"
                     /db_xref="CDD:270796"
     Site            order(1089..1093,1097,1110,1112,1143,1159..1162,
                     1164..1166,1169,1210..1211,1213,1224..1226)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270796"
     Site            order(1092..1093,1206,1208..1210,1228,1245..1248,1250,
                     1280,1289)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270796"
     Site            order(1224..1233,1244..1250)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270796"
     CDS             1..1361
                     /gene="Mekk1"
                     /coded_by="XM_034329356.2:1783..5868"
                     /db_xref="GeneID:117606641"
ORIGIN      
        1 mlgydkrshd aenlfsgssd ddtnyvgkkg sgsstnefde siqamcdmyg qtpprtrlnr
       61 kkrymkqkln ggdtksldke tpskllvrrr ntldsiifne mcdkadrdce neanqdmvke
      121 gsrkckrifk vlrgserdlk ldmkaaydya ekfdhgqvst pnhikvetkn rfrclrskpv
      181 gveerrvetd epnlvidnne svaqspktls lkereifhdt fsflirlgst erncrrqish
      241 eetswqnelk dliwlelqah hadrnpmaqd eylckqreal egllkeimey rydpiasvkn
      301 gntpwinisg kcvdrvvnsg sspnielgic pgclsmycra caraqgealq qvegllarle
      361 vaeslypssq alavqyplyk steftgrvka mclwynmtkh hrlklqilgk llmmlhtkkk
      421 hddngdsgis srssdtvdss ehrrtlvrfe vsnpqeesss psdsnnsnns svglsfaqsw
      481 pstpetsltc ldddkterld fylvefdrqa yrkyiedvlk trglrkslnf lerlhtsvlr
      541 karltleksd vseggnatse eyegdeelrr ygvwsteare lnlpsyraaf vflsrvpldv
      601 vheflrmrle qkpdqpspls vrqlmrelre glriacihrd rfsahscaav asdnescdql
      661 lfqedvkdfd dslkavfevy ldylqqwvdm vqhdsfhknl imdewifvks iagnilngyk
      721 iagvkfceia ktmikqvkef lnerpreike ttedwsdddd wsckfrfhfm fvgrewqgmf
      781 vesrekvvks vtlarclkrd iekwaffnkd leesltilke tvmslrhqit vvitnfqrdl
      841 ekaeepnseg drfafrsrvr eilhqayrmg fdyyketckl fsleekavla rglvsfaflw
      901 mefvrtrcer grglrprwan qgleflitvc epqntkhlsd qefeelktcm drcishvvgs
      961 aniasnvete sirrlsshsr asspcharsr tasisqkprd afkspdvpsn pkkspspsiv
     1021 dgnimlntfd rsisrqeriv rairkvdcdi derlksreli gqvtdrkavd rvhikarrvt
     1081 ftwqrgikig qgrfgkvytv vnnqtgella mkevqlqpgd hrairrvaee lqifegiqhk
     1141 hlvryyglei hreemlifme fcaegtlesl vagsgnglpe slvrkythql llavavlhch
     1201 givhrdiksa nifltdegnc lklgdfgsav qikahttmpg elqgfvgtqa ymapevfmks
     1261 essghgraad iwsvgccvie masgrrpwsd ydsnyqimfk vgmgetpalp kslsaegidl
     1321 ikkclqhdpk rrltansllt lpfaqayedv nadlvvarhq t
//
LOCUS       XP_034185248            1359 aa            linear   INV 03-SEP-2025
DEFINITION  mitogen-activated protein kinase kinase kinase 4 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185248
VERSION     XP_034185248.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329357.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1359
                     /product="mitogen-activated protein kinase kinase kinase 4
                     isoform X2"
                     /calculated_mol_wt=155625
     Region          28..>805
                     /region_name="MEKK4_N"
                     /note="MEKK4 N-terminal; pfam19431"
                     /db_xref="CDD:466077"
     Region          1081..1342
                     /region_name="STKc_MEKK4"
                     /note="Catalytic domain of the Protein Serine/Threonine
                     Kinase, Mitogen-Activated Protein (MAP)/Extracellular
                     signal-Regulated Kinase (ERK) Kinase Kinase 4; cd06626"
                     /db_xref="CDD:270796"
     Site            order(1087..1091,1095,1108,1110,1141,1157..1160,
                     1163..1164,1167,1204,1206..1209,1211,1222..1223,1226,
                     1243..1246,1248,1278,1287)
                     /site_type="active"
                     /db_xref="CDD:270796"
     Site            order(1087..1091,1095,1108,1110,1141,1157..1160,
                     1162..1164,1167,1208..1209,1211,1222..1224)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270796"
     Site            order(1090..1091,1204,1206..1208,1226,1243..1246,1248,
                     1278,1287)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270796"
     Site            order(1222..1231,1242..1248)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270796"
     CDS             1..1359
                     /gene="Mekk1"
                     /coded_by="XM_034329357.2:1783..5862"
                     /db_xref="GeneID:117606641"
ORIGIN      
        1 mlgydkrshd aenlfsgssd ddtnyvgkkg sgsstnefde siqamcdmyg qtpprtrlnr
       61 kkrymkqkln ggdtksldke tpskllvrrr ntldsiifne mcdkadrdce neanqdmvke
      121 gsrkckrifk vlrgserdlk ldmkaaydya ekfdhgqvst pnhikvetkn rfrclrskpv
      181 gveerrvetd epnlvidnne svaqspktls lkereifhdt fsflirlgst erncrrqish
      241 eetswqnelk dliwlelqah hadrnpmaqd eylckqreal egllkeimey rydpiasvkn
      301 gntpwinisg kcvdrvvnsg sspnielgic pgclsmycra caraqgealq qvegllarle
      361 vaeslypssq alavqyplyk steftgrvka mclwynmtkh hrlklqilgk llmmlhtkkk
      421 hddngdsgis srssdtvdss ehrrtlvrfe vsnpqeesss psdsnnsnns svglsfaqsw
      481 pstpetsltc ldddkterld fylvefdrqa yrkyiedvlk trglrkslnf lerlhtsvlr
      541 karltleksd vseggnatse eyegdeelrr ygvwsteare lnlpsyraaf vflsrvpldv
      601 vheflrmrle qkpdqpspls vrqlmrelre glriacihrd rfsahscaav asdnescdql
      661 lfqedvkdfd dslkavfevy ldylqqwvdm vqhdsfhknl imdewifvks iagnilngyk
      721 iagvkfceia ktmikqvkef lnerpreike ttedwsdddd wsckfhfmfv grewqgmfve
      781 srekvvksvt larclkrdie kwaffnkdle esltilketv mslrhqitvv itnfqrdlek
      841 aeepnsegdr fafrsrvrei lhqayrmgfd yyketcklfs leekavlarg lvsfaflwme
      901 fvrtrcergr glrprwanqg leflitvcep qntkhlsdqe feelktcmdr cishvvgsan
      961 iasnvetesi rrlsshsras spcharsrta sisqkprdaf kspdvpsnpk kspspsivdg
     1021 nimlntfdrs isrqerivra irkvdcdide rlksreligq vtdrkavdrv hikarrvtft
     1081 wqrgikigqg rfgkvytvvn nqtgellamk evqlqpgdhr airrvaeelq ifegiqhkhl
     1141 vryygleihr eemlifmefc aegtleslva gsgnglpesl vrkythqlll avavlhchgi
     1201 vhrdiksani fltdegnclk lgdfgsavqi kahttmpgel qgfvgtqaym apevfmkses
     1261 sghgraadiw svgccviema sgrrpwsdyd snyqimfkvg mgetpalpks lsaegidlik
     1321 kclqhdpkrr ltanslltlp faqayedvna dlvvarhqt
//
LOCUS       XP_034185249            1144 aa            linear   INV 03-SEP-2025
DEFINITION  nardilysin [Osmia lignaria lignaria].
ACCESSION   XP_034185249
VERSION     XP_034185249.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329358.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185249.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1144
                     /product="nardilysin"
                     /calculated_mol_wt=131836
     Region          100..1079
                     /region_name="Ptr"
                     /note="Secreted/periplasmic Zn-dependent peptidases,
                     insulinase-like [Posttranslational modification, protein
                     turnover, chaperones]; COG1025"
                     /db_xref="CDD:440648"
     CDS             1..1144
                     /gene="LOC117606642"
                     /coded_by="XM_034329358.2:128..3562"
                     /db_xref="GeneID:117606642"
ORIGIN      
        1 mklyvtcssr ylvtkvlqcg skkffhstrm lkrslqsppe kklkpnqsts nsiknlntlt
       61 ehnhstsmvv dnmqsenqve neenslmdts qikvtyldpp vksendkkey rviqlenglt
      121 alliadlhsp gsqnnnankg aeitskdeev tseeesgsed esenestdde cgcdgkdsts
      181 sskrlkrdek maacglsigv gsfsdppeip glahflehmv fmgsekypqe ndfdafikkr
      241 ggsdnactdc esttyyfeiq eihllaaldr faqffinplm kkdaitrere avesefqmal
      301 psddnrkeqi fgslakanhp srkfgwgnlv tlrdnvseek lyeelhkfre rhysahrmkv
      361 aiqaklpldv lesyvkqcfv nvrnnglpad dfsmfkgada fhtpnfrriy kmkpirdvrq
      421 vdltwslppv qhlykskphe yiswvlghkg kgslmsylkk kmwcldidid nadsgfadts
      481 myalftislt lteegqkqlp evlnaifsfi nlmqregpqk rifdemqqim etnfrfldei
      541 ppadyvedlc edmhyypprd yltggdlyfe ydpegieaym kcltaedvni iildkkfnee
      601 efekvepwfk tkytdieipk ewierwrtie plaefhlplp nvfltddftl ipipsdvsky
      661 pikihsddit evwyrpdpkf cvpecymyfn ivipmaissp ksaallelfv lilkqllvek
      721 lypatvarln semytsdrgi llkvhgfnqk lpllltiiak yiadsanlvs eelfeamkek
      781 tiqeyynifl kpkklvtdvg lsilmlvhwp aldkhvairn vqyeefrnfi kyvtdhvyiq
      841 slvqgntrke dvvknvkefv etlkcgplla ntmpqiriaq iplgsycckv knfnktdvns
      901 vvmnyyqsgi tsirlsviie liimimeepl fnqlrtletl gynvfcllrd tfgilgysvt
      961 vhtqadkfst ehaanrieaf lqmfngilke msdedlesik ealtkqklcd dvqlkeevnr
     1021 nwpeivsgny mfdriekell mieqitieel rewmishtln gknlrklsih vvgtsepken
     1081 ndseldlneq nsnstlekva nvkypltylp iteeledkss vhcitdiqey ksrlycypsk
     1141 htpi
//
LOCUS       XP_034185250            1082 aa            linear   INV 03-SEP-2025
DEFINITION  HECT and RLD domain containing E3 ubiquitin ligase 4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185250
VERSION     XP_034185250.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329359.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185250.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1082
                     /product="HECT and RLD domain containing E3 ubiquitin
                     ligase 4"
                     /calculated_mol_wt=122051
     Region          24..366
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          341..407
                     /region_name="RCC1"
                     /note="Regulator of chromosome condensation (RCC1) repeat;
                     pfam00415"
                     /db_xref="CDD:395335"
     Region          737..1080
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(741,771,782,839,1016,1044..1045,1048..1052,1072,
                     1079)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(867,870..871,873..874,877,886,888,892..893,895,900,
                     905,922,926)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..1082
                     /gene="Herc4"
                     /coded_by="XM_034329359.2:169..3417"
                     /db_xref="GeneID:117606643"
ORIGIN      
        1 manwnkkrrl ayepviavey tsftdkdmfc wgstihgelg lggiedenil vprevdfkka
       61 teieqiacge nytvvitqdg evyscgnndy gqlghekgrk rlqlipglda fvfkkiacga
      121 rhtlavnewg qlfswgsnae gqlglnsknl iepvprmvkt lgtsivvqva cgmshvlalt
      181 ndgklyswgs nsegqlglgt dvrneikpkl isclagvpis liacggyhsl aisksgavfg
      241 wgkntfgqlg lndtrsrnlp cqlqtlqnar vcyaacgeef svfltvdggv ftcgagmygq
      301 lghgsntnei lprqvmelmg stvtqiscgk rhtlglvpsr grvyawglgg agqlgnsvtr
      361 svpvpqvvhg pwvapngssm mdldglnsyk inyvvkhift ggdhcfatvv prdtniepdd
      421 crilqpssqi lviteeelma cqrvppnspv dhelmtylet vfksescvtg sfllnkdahy
      481 gctkkhhgvd lddasrlfgi irelvnetiq eliftcites lipsftssny ftlkavcpea
      541 lriylilply hgfanplnct vlhtpfckav lslkpeilei itswwlgast hyferlvrih
      601 kfivlhyvks kinkeviwdd tlqvvldllh llnklnnegd agnkvpystf hvpelieyid
      661 irgdyikwtl gpssyrrvfc hypflldaqa ktilletdqa iqmqsamnea atravmnqmf
      721 fdpfsvdsrq hnqfvmlnvs renivadtlm elshydssdl kkplrvkfhg eeaedaggvk
      781 keffmlllke ildpkygmfk qyeesriiwf sedsledenm yfligilcgl viynfiiidl
      841 pfplalykkl lhepvglndi kemspllaks mqnildyede dfeeifclhf evvrevfgek
      901 rifelipggs kvpvtlnnkk qfvdlyvdyi lnksvephfd kfhkgfhkvc ggrvlelfhs
      961 helmavvvgn edydweelek natykegytk sdptiimfwq vfreltleek kkfllfltgs
     1021 dripiqgmqa ikitiqpmhd erllpvahtc fnlldlpryq trerlrykll qaiqqthgfs
     1081 lv
//
LOCUS       XP_034185251             969 aa            linear   INV 03-SEP-2025
DEFINITION  constitutive coactivator of PPAR-gamma-like protein 1 homolog
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185251
VERSION     XP_034185251.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329360.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..969
                     /product="constitutive coactivator of PPAR-gamma-like
                     protein 1 homolog isoform X1"
                     /calculated_mol_wt=108146
     Region          5..224
                     /region_name="PIN_FAM120B-like"
                     /note="FEN-like PIN domains of FAM120B (family with
                     sequence similarity 120B) and related proteins; cd18672"
                     /db_xref="CDD:350239"
     Site            order(109..117,139..147)
                     /site_type="other"
                     /note="helical arch"
                     /db_xref="CDD:350239"
     Region          <686..>768
                     /region_name="PLPDE_III"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzymes; cl00261"
                     /db_xref="CDD:469695"
     CDS             1..969
                     /gene="LOC117606644"
                     /coded_by="XM_034329360.2:327..3236"
                     /db_xref="GeneID:117606644"
ORIGIN      
        1 mgiqdlqvfl dgnfvqggsv pvdllkiart viqrqhnrig ktqqlqsgkl rlvldgeccl
       61 drlyggyfsd wacggqwnrm lqflvvliha teasrlelav ffngcfespr radwiqnqlq
      121 vrikvnnvlk histkgtppp kiwwtppvcl rtslrmalrh lnvtvlcsmd dhhqeviayc
      181 rennfnglia ddaeyaafdp pryfsseqlk ltykgsldtk eyilpdvlks lnipsdrlcl
      241 laallgnyil tetdladfyk klnvnasfnk vnvehtikti anyvrdlpsv epdvvaqkvf
      301 gslsdprcsk lrqsvqyyin gtkegflhyk pvkpnrdvvs vhkidskqnv qqqsnlsetp
      361 stsevdsnle tsrfasetve sereslnayk qatanaksap qividppglq ghgdadnirs
      421 edeqsnghai ngthnlliss ssssssssat spshatadsg kqgekitnip ttvpevmrta
      481 serhqkglms phiyqiliag eiklpvlled enhrefpsih liyrpvrqmv yailfnlhhr
      541 mylaakskek gdsekievpd vvikewvwsk snpyqtpelv kaeqigwgvp tiqrlwfgtg
      601 iddkrrrlrg fltcmrsdtp lmlntsyvpq hllvmacvlr yimsfsdkik ilrrqeldaf
      661 iaqafspelm naqylqdlql plvtsrgvql atlfmtgvet allandacga pipwlmccpw
      721 lyfdgklfhh tlartmiakn llelcsgyid rvvkvermrk avleginviy artplpvsra
      781 ipvsvpqnil sgtmpqrglm rrpipsrggq leiagvvvgq wgpnygqpgr lpqplqghir
      841 grippqvtsi gglkyglprg fnpmgrptfq trgtnrtqla grgkktqmka tgkkkatvkk
      901 nkenekkenr grgnnidgit dyndtipnmn atkdalpvpg qfedavensk giekgqgdas
      961 lvvpvvaen
//
LOCUS       XP_034185252             969 aa            linear   INV 03-SEP-2025
DEFINITION  constitutive coactivator of PPAR-gamma-like protein 1 homolog
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185252
VERSION     XP_034185252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329361.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..969
                     /product="constitutive coactivator of PPAR-gamma-like
                     protein 1 homolog isoform X1"
                     /calculated_mol_wt=108146
     Region          5..224
                     /region_name="PIN_FAM120B-like"
                     /note="FEN-like PIN domains of FAM120B (family with
                     sequence similarity 120B) and related proteins; cd18672"
                     /db_xref="CDD:350239"
     Site            order(109..117,139..147)
                     /site_type="other"
                     /note="helical arch"
                     /db_xref="CDD:350239"
     Region          <686..>768
                     /region_name="PLPDE_III"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzymes; cl00261"
                     /db_xref="CDD:469695"
     CDS             1..969
                     /gene="LOC117606644"
                     /coded_by="XM_034329361.2:327..3236"
                     /db_xref="GeneID:117606644"
ORIGIN      
        1 mgiqdlqvfl dgnfvqggsv pvdllkiart viqrqhnrig ktqqlqsgkl rlvldgeccl
       61 drlyggyfsd wacggqwnrm lqflvvliha teasrlelav ffngcfespr radwiqnqlq
      121 vrikvnnvlk histkgtppp kiwwtppvcl rtslrmalrh lnvtvlcsmd dhhqeviayc
      181 rennfnglia ddaeyaafdp pryfsseqlk ltykgsldtk eyilpdvlks lnipsdrlcl
      241 laallgnyil tetdladfyk klnvnasfnk vnvehtikti anyvrdlpsv epdvvaqkvf
      301 gslsdprcsk lrqsvqyyin gtkegflhyk pvkpnrdvvs vhkidskqnv qqqsnlsetp
      361 stsevdsnle tsrfasetve sereslnayk qatanaksap qividppglq ghgdadnirs
      421 edeqsnghai ngthnlliss ssssssssat spshatadsg kqgekitnip ttvpevmrta
      481 serhqkglms phiyqiliag eiklpvlled enhrefpsih liyrpvrqmv yailfnlhhr
      541 mylaakskek gdsekievpd vvikewvwsk snpyqtpelv kaeqigwgvp tiqrlwfgtg
      601 iddkrrrlrg fltcmrsdtp lmlntsyvpq hllvmacvlr yimsfsdkik ilrrqeldaf
      661 iaqafspelm naqylqdlql plvtsrgvql atlfmtgvet allandacga pipwlmccpw
      721 lyfdgklfhh tlartmiakn llelcsgyid rvvkvermrk avleginviy artplpvsra
      781 ipvsvpqnil sgtmpqrglm rrpipsrggq leiagvvvgq wgpnygqpgr lpqplqghir
      841 grippqvtsi gglkyglprg fnpmgrptfq trgtnrtqla grgkktqmka tgkkkatvkk
      901 nkenekkenr grgnnidgit dyndtipnmn atkdalpvpg qfedavensk giekgqgdas
      961 lvvpvvaen
//
LOCUS       XP_034185255             965 aa            linear   INV 03-SEP-2025
DEFINITION  constitutive coactivator of PPAR-gamma-like protein 1 homolog
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185255
VERSION     XP_034185255.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329364.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..965
                     /product="constitutive coactivator of PPAR-gamma-like
                     protein 1 homolog isoform X2"
                     /calculated_mol_wt=107745
     Region          5..224
                     /region_name="PIN_FAM120B-like"
                     /note="FEN-like PIN domains of FAM120B (family with
                     sequence similarity 120B) and related proteins; cd18672"
                     /db_xref="CDD:350239"
     Site            order(109..117,139..147)
                     /site_type="other"
                     /note="helical arch"
                     /db_xref="CDD:350239"
     Region          <682..>764
                     /region_name="PLPDE_III"
                     /note="Type III Pyridoxal 5-phosphate (PLP)-Dependent
                     Enzymes; cl00261"
                     /db_xref="CDD:469695"
     CDS             1..965
                     /gene="LOC117606644"
                     /coded_by="XM_034329364.2:327..3224"
                     /db_xref="GeneID:117606644"
ORIGIN      
        1 mgiqdlqvfl dgnfvqggsv pvdllkiart viqrqhnrig ktqqlqsgkl rlvldgeccl
       61 drlyggyfsd wacggqwnrm lqflvvliha teasrlelav ffngcfespr radwiqnqlq
      121 vrikvnnvlk histkgtppp kiwwtppvcl rtslrmalrh lnvtvlcsmd dhhqeviayc
      181 rennfnglia ddaeyaafdp pryfsseqlk ltykgsldtk eyilpdvlks lnipsdrlcl
      241 laallgnyil tetdladfyk klnvnasfnk vnvehtikti anyvrdlpsv epdvvaqkvf
      301 gslsdprcsk lrqsvqyyin gtkegflhyk pvkpnrvhki dskqnvqqqs nlsetpstse
      361 vdsnletsrf asetvesere slnaykqata naksapqivi dppglqghgd adnirsedeq
      421 snghaingth nllissssss ssssatspsh atadsgkqge kitnipttvp evmrtaserh
      481 qkglmsphiy qiliageikl pvlledenhr efpsihliyr pvrqmvyail fnlhhrmyla
      541 akskekgdse kievpdvvik ewvwsksnpy qtpelvkaeq igwgvptiqr lwfgtgiddk
      601 rrrlrgfltc mrsdtplmln tsyvpqhllv macvlryims fsdkikilrr qeldafiaqa
      661 fspelmnaqy lqdlqlplvt srgvqlatlf mtgvetalla ndacgapipw lmccpwlyfd
      721 gklfhhtlar tmiaknllel csgyidrvvk vermrkavle ginviyartp lpvsraipvs
      781 vpqnilsgtm pqrglmrrpi psrggqleia gvvvgqwgpn ygqpgrlpqp lqghirgrip
      841 pqvtsigglk yglprgfnpm grptfqtrgt nrtqlagrgk ktqmkatgkk katvkknken
      901 ekkenrgrgn nidgitdynd tipnmnatkd alpvpgqfed avenskgiek gqgdaslvvp
      961 vvaen
//
LOCUS       XP_076545628             943 aa            linear   INV 03-SEP-2025
DEFINITION  integrator complex subunit 5 omd isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545628
VERSION     XP_076545628.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689513.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..943
                     /product="integrator complex subunit 5 omd isoform X1"
                     /calculated_mol_wt=105660
     Region          11..213
                     /region_name="INTS5_N"
                     /note="Integrator complex subunit 5 N-terminus; pfam14837"
                     /db_xref="CDD:464341"
     Region          226..858
                     /region_name="INTS5_C"
                     /note="Integrator complex subunit 5 C-terminus; pfam14838"
                     /db_xref="CDD:464342"
     CDS             1..943
                     /gene="omd"
                     /coded_by="XM_076689513.1:336..3167"
                     /db_xref="GeneID:117606636"
ORIGIN      
        1 mlhstgtlsp qdilsevrkf isgavrpths tstldvtrta lsllrnvpaa rdavleyfcn
       61 vffvavtkhv rqietnqnlt iceetiiaei htvlssfing npeawapiis awslellgkl
      121 ssdyakrgnl pvnagindfl qqwmscratr tliditaqcl qclmhsdtes cikalldtsv
      181 lhsphfdwvv ahvgscfpnt vitrvlscgl kdfftmdhkh niknpklnsv vgilghlags
      241 hfqdirtall dlfkwsleed mnvdedtkkq klatvpflln laslsqtllk aitsdvlqtl
      301 kpdiipqlal fasdwckyfd nqpealidlt vhlilgceqg asqiinilld ttlntsnvgy
      361 hsvnaaqsvk nvcseilelv lqeidlllrt hgpqsanial lnsikqelpv ilplllnpnp
      421 lrvqtavrll cflgsqnsnt lisaasyvlv kaattfhlaa lirlisnniv lfsstksete
      481 nalashgyft qvveqalrei nyksviekqe trqlfqnlti llkweksnka sifrskmisq
      541 aiklnlyqis allmktgdfn landiaklld lfsmpekdhf apnieltlkl traiiqyffl
      601 ciaenditkk qqgvrivchl lkdltsyspc arvlalrefl ghginndaak cfgakekfep
      661 kfeetlllhq nhkqvtstml aqkhssvfha gvighgprrp ppensldkdt itlnkillmd
      721 vikaccnnre serypvnlda ltmvslllve lvspdvmyng lpwpdeeftk vtierdlqir
      781 rtfkdvpllw tlleltawyr palaycsvll rgiaatvman wnteegvslv svmalgqllp
      841 pplasirdil pvlephqvpf ydetalntli atmeplkipl vlllpsqifk ilhyifihnf
      901 kylipvfpvn pltakflfni lfniillqvi kkiyiqipiw gsw
//
LOCUS       XP_034185233             930 aa            linear   INV 03-SEP-2025
DEFINITION  integrator complex subunit 5 omd isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185233
VERSION     XP_034185233.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329342.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..930
                     /product="integrator complex subunit 5 omd isoform X2"
                     /calculated_mol_wt=103882
     Region          11..213
                     /region_name="INTS5_N"
                     /note="Integrator complex subunit 5 N-terminus; pfam14837"
                     /db_xref="CDD:464341"
     Region          226..917
                     /region_name="INTS5_C"
                     /note="Integrator complex subunit 5 C-terminus; pfam14838"
                     /db_xref="CDD:464342"
     CDS             1..930
                     /gene="omd"
                     /coded_by="XM_034329342.2:329..3121"
                     /db_xref="GeneID:117606636"
ORIGIN      
        1 mlhstgtlsp qdilsevrkf isgavrpths tstldvtrta lsllrnvpaa rdavleyfcn
       61 vffvavtkhv rqietnqnlt iceetiiaei htvlssfing npeawapiis awslellgkl
      121 ssdyakrgnl pvnagindfl qqwmscratr tliditaqcl qclmhsdtes cikalldtsv
      181 lhsphfdwvv ahvgscfpnt vitrvlscgl kdfftmdhkh niknpklnsv vgilghlags
      241 hfqdirtall dlfkwsleed mnvdedtkkq klatvpflln laslsqtllk aitsdvlqtl
      301 kpdiipqlal fasdwckyfd nqpealidlt vhlilgceqg asqiinilld ttlntsnvgy
      361 hsvnaaqsvk nvcseilelv lqeidlllrt hgpqsanial lnsikqelpv ilplllnpnp
      421 lrvqtavrll cflgsqnsnt lisaasyvlv kaattfhlaa lirlisnniv lfsstksete
      481 nalashgyft qvveqalrei nyksviekqe trqlfqnlti llkweksnka sifrskmisq
      541 aiklnlyqis allmktgdfn landiaklld lfsmpekdhf apnieltlkl traiiqyffl
      601 ciaenditkk qqgvrivchl lkdltsyspc arvlalrefl ghginndaak cfgakekfep
      661 kfeetlllhq nhkqvtstml aqkhssvfha gvighgprrp ppensldkdt itlnkillmd
      721 vikaccnnre serypvnlda ltmvslllve lvspdvmyng lpwpdeeftk vtierdlqir
      781 rtfkdvpllw tlleltawyr palaycsvll rgiaatvman wnteegvslv svmalgqllp
      841 pplasirdil pvlephqini imkecvwaym renvpspalf trseganiaw rdtdtsapng
      901 rfteilrlvl lsnihtlgsl yatlfynenk
//
LOCUS       XP_034185256             800 aa            linear   INV 03-SEP-2025
DEFINITION  heat shock protein 90 Gp93 [Osmia lignaria lignaria].
ACCESSION   XP_034185256
VERSION     XP_034185256.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329365.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..800
                     /product="heat shock protein 90 Gp93"
                     /calculated_mol_wt=91653
     Region          83..274
                     /region_name="HATPase_Hsp90-like"
                     /note="Histidine kinase-like ATPase domain of human
                     cytosolic Hsp90 and its homologs including Escherichia
                     coli HtpG, and related domains; cd16927"
                     /db_xref="CDD:340404"
     Site            order(84..85,88,92,167,194)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:340404"
     Site            order(103,107..108,110..111,114,147,149,151,153..154,
                     162..163,165..171,192..198,210,212,243..244,246)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:340404"
     Site            order(163,198)
                     /site_type="other"
                     /note="ATP-lid"
                     /db_xref="CDD:340404"
     Region          256..758
                     /region_name="HSP90"
                     /note="Hsp90 protein; pfam00183"
                     /db_xref="CDD:459703"
     CDS             1..800
                     /gene="Gp93"
                     /coded_by="XM_034329365.2:115..2517"
                     /db_xref="GeneID:117606645"
ORIGIN      
        1 mkkalilisi lffvagfvra edevdenvgt vendlgasre gsrtddgvvq reeeaikldg
       61 lnvaqikelr ekaekfmfqt evnrmmklii nslyrnkdif lrelisnasd aldkirllsl
      121 tdknvldtnp elairiksdk enkilsitds gigmtkkdli tnlgtiaksg taeflgkmqe
      181 asnnqdlndm igqfgvgfys aflvsntviv tskhnddkqy iwqsdsnsys ivedprgdtl
      241 krgttvslhl kdealdflee dtirnlvkky sqfinfpiyl wsskvvqvde eedeqakdde
      301 skkdepsted kvteeeddak vedaeeekkt kkvektvwdw ellndskpiw tlkpseveek
      361 dynefyktlt kdtqdplaki hfiaegevtf ksllfipkvq psdsfnrygt kadniklyvr
      421 rvfitdkftd mmpnylsfir givdsddlpl nvsrenlqqh klikvikkkl irkvldmikk
      481 ipkeeyekfw keystniklg viedaqnrar lskllsfqss tqkgmtslsd yvsrmkpnqq
      541 hiyyiagsse eevkkspfve rldkkgyevl ylteavdeyt isalpefdgk kfqnvakegf
      601 tldegekake rmeqlkstfe plvkwlndil kdhvskalvs erltdspcal vasmfgwtgn
      661 merlaisnah qksddpqkay ylnqkktlei nprhplirel lrrvevdtad qtakdialmm
      721 frtatlrsgy mlretasfad sveqlmrktl gisldevpee eeaqeeesas aeteagsese
      781 kimdmdenee gkdeekhdel
//
LOCUS       XP_076545629             618 aa            linear   INV 03-SEP-2025
DEFINITION  choline acetyltransferase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545629
VERSION     XP_076545629.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689514.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..618
                     /product="choline acetyltransferase isoform X1"
                     /calculated_mol_wt=69738
     Region          1..603
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..618
                     /gene="ChAT"
                     /coded_by="XM_076689514.1:134..1990"
                     /db_xref="GeneID:117606654"
ORIGIN      
        1 mqkyltqvev iapdhleknr ilvraflsgp gpklqqrlle rkqkvanwat awwlndmyls
       61 iplplpinvn pglvtkpktf stqkgaavfl arfltellny qelldsggle vqrvkskdgk
      121 amqpmcmaqh yqmfriyrrp gingdeqvil dratsgdhii vahhnqfysv pvrapdrgri
      181 teaelvqqil simetkadpr tppvgiltta erpiwakare ellknernrh nlellekclc
      241 vvcidddvlp atfnnpynke drwigdrdya nvlhhalhgg gsrhlganrw fdktvhailg
      301 kdgmwgltye hsageaasif dafeeltakv dampppeent ipshlpapek ltwqlseqsl
      361 ndirdamtsf dalvedldlc ifwfkdyske fikssrispd vyiqlalqft hfrlhgnlva
      421 tyesagirrf algrvdcira aspevlnwak amcqgdpesq ttnqqnsgpd egtkrvqfti
      481 ysqerikelf dlavqrqtke mtdnitgqgi dnhlmglryt akevgepipe lftnqayeii
      541 nhfslstsqv ttkndaivgy gpvvpdgygc aynirnngii fsvsafhsdg rtnatrfart
      601 leqslrdmsa mlkqagqr
//
LOCUS       XP_076545630             611 aa            linear   INV 03-SEP-2025
DEFINITION  choline acetyltransferase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545630
VERSION     XP_076545630.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689515.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..611
                     /product="choline acetyltransferase isoform X2"
                     /calculated_mol_wt=68899
     Region          1..596
                     /region_name="Carn_acyltransf"
                     /note="Choline/Carnitine o-acyltransferase; pfam00755"
                     /db_xref="CDD:459926"
     CDS             1..611
                     /gene="ChAT"
                     /coded_by="XM_076689515.1:134..1969"
                     /db_xref="GeneID:117606654"
ORIGIN      
        1 mqkyltqvev iapdhleknr ilvraflsgp gpklqqrlle rkqkvanwat awwlndmyls
       61 iplplpinvn pglvtkpktf stqkgaavfl arfltellny qelldsggle vqrvkskdgk
      121 amqpmcmaqh yqmfriyrrp gingdeqvil dratsgdhii vahhnqfysv pvrapdrgri
      181 teaelvqqil simetkadpr tppvgiltta erpiwakare ellknernrh nlellekclc
      241 vvcidddvlp atfnnpynke drwigdrdya nvlhhalhgg gsrhlganrw fdktvhailg
      301 kdgmwgltye hsageaasif dafeeltakv dampppeent ipshlpapek ltwqlseqsl
      361 ndirdamtsf dalvedldlc ifwfkdyske fikssrispd vyiqlalqft hfrlhgnlva
      421 tyesagirrf algrvdcira aspevlnwak amcqgdpesq ttnqqnsgpd egtkrqerik
      481 elfdlavqrq tkemtdnitg qgidnhlmgl rytakevgep ipelftnqay eiinhfslst
      541 sqvttkndai vgygpvvpdg ygcaynirnn giifsvsafh sdgrtnatrf artleqslrd
      601 msamlkqagq r
//
LOCUS       XP_034185257             585 aa            linear   INV 03-SEP-2025
DEFINITION  vesicular acetylcholine transporter [Osmia lignaria lignaria].
ACCESSION   XP_034185257
VERSION     XP_034185257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="vesicular acetylcholine transporter"
                     /calculated_mol_wt=64465
     Region          34..435
                     /region_name="MFS_SLC18A3_VAChT"
                     /note="Vesicular acetylcholine transporter (VAChT) and
                     similar transporters of the Major Facilitator Superfamily;
                     cd17383"
                     /db_xref="CDD:340941"
     Site            order(44..45,48..49,52,103,153..154,156..158,161,182,
                     185..186,189,263,266..267,270..272,275,303,307,356..357,
                     361,365,386,389..390,393..394,397)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340941"
     CDS             1..585
                     /gene="VAChT"
                     /coded_by="XM_034329366.2:307..2064"
                     /db_xref="GeneID:117606646"
ORIGIN      
        1 mtsippifki dlnelkelvw tkvqepkaqr klvlaivsia llldnmlymv ivpiipdylr
       61 yigafddder edlnatvapg hhgqdsatgv lfaskaivql mvnpfsgali drigydipmm
      121 iglcimflst avfacgrsyg vlffarslqg vgsafadtsg lamianryte egerskalgi
      181 alafisfgcl vappfggaly qfagkevpfl ilafisladg fmlllvmkpl keqmkesqqp
      241 rpptipiwrl lmdpyiavca galmmsnval afleptisiw mednithdnw kmgmiwlpaf
      301 fphvfgviit vkmakqypqh qwliaasgla legfccfiip fctgykvlmi plcgicfgma
      361 lidtallptl gylvdvryvs vygsiyaiad isysvayavg piiaggvvea igftalnvgi
      421 afsnllyapv lmylrhiydf kpfqdeadvl mqdppdkeyq tyvlqeqrpl sgevgnhlnq
      481 trmetsldqg ydqnyqggqg ydqsgygqni nasgysqapa gyeqppmydq qtnysqqstg
      541 ygqqrasygq epqtagqmdv npfrrptadq qqpagdsnpf rqgmy
//
LOCUS       XP_034185258             576 aa            linear   INV 03-SEP-2025
DEFINITION  moesin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185258
VERSION     XP_034185258.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329367.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..576
                     /product="moesin isoform X1"
                     /calculated_mol_wt=68068
     Region          10..209
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          203..299
                     /region_name="FERM_C_ERM"
                     /note="FERM domain C-lobe/F3 of the ERM family; cd13194"
                     /db_xref="CDD:270015"
     Site            order(245..246,248..254,263,284,287..288,291)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(245,248..255,263,284,287..288,291)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(281,285)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270015"
     Site            284..299
                     /site_type="other"
                     /note="actin binding site 2 [polypeptide binding]"
                     /db_xref="CDD:270015"
     Region          333..450
                     /region_name="ERM_helical"
                     /note="Ezrin/radixin/moesin, alpha-helical domain;
                     pfam20492"
                     /db_xref="CDD:466641"
     Region          502..576
                     /region_name="ERM_C"
                     /note="Ezrin/radixin/moesin family C terminal; pfam00769"
                     /db_xref="CDD:459932"
     CDS             1..576
                     /gene="Moe"
                     /coded_by="XM_034329367.2:220..1950"
                     /db_xref="GeneID:117606647"
ORIGIN      
        1 mvagakmmnv rvttmdaele faiqqtttgk qlfdqvvkti glrevwffgl qytdnkgdlt
       61 wiklykkvmn qevkketplq fkfrakfype dvaeeliqdi tlrlfylqvk nailtdeiyc
      121 ppetsvllas yavqarhgdy qkgthtagfl mndrlvpqrv vdqhkmskee wessitnwwq
      181 ehrgmlreda mmeylkiaqd lemygvnyfe irnkkgtdlw lgvdalglni yekdnkltpk
      241 igfpwseirn isfndkkfii kpidkkapdf vffatrvkin krilalcmgn helymrrrkp
      301 dtidvqqmka qareekiakq qqreklqlei aareraekkq qeyeerlrnm aeemdrrqae
      361 lneaqemirr leeqlkqlqa akeeledrqk eltammekle lshemeaaer akleqeirak
      421 qeevqriqse veakdaearr lqeefeaanr lrqeeadray qanttphhhh veendegeee
      481 gedevphgdv tkdlatdesi idpveerrtl aernerlhdq lkalkqdlaq srdesketvm
      541 dkihrenvrq grdkyktlre irkgntkrrv dqfenm
//
LOCUS       XP_034185259             575 aa            linear   INV 03-SEP-2025
DEFINITION  moesin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185259
VERSION     XP_034185259.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329368.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="moesin isoform X2"
                     /calculated_mol_wt=67926
     Region          10..209
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          203..299
                     /region_name="FERM_C_ERM"
                     /note="FERM domain C-lobe/F3 of the ERM family; cd13194"
                     /db_xref="CDD:270015"
     Site            order(245..246,248..254,263,284,287..288,291)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(245,248..255,263,284,287..288,291)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(281,285)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270015"
     Site            284..299
                     /site_type="other"
                     /note="actin binding site 2 [polypeptide binding]"
                     /db_xref="CDD:270015"
     Region          333..452
                     /region_name="ERM_helical"
                     /note="Ezrin/radixin/moesin, alpha-helical domain;
                     pfam20492"
                     /db_xref="CDD:466641"
     Region          501..575
                     /region_name="ERM_C"
                     /note="Ezrin/radixin/moesin family C terminal; pfam00769"
                     /db_xref="CDD:459932"
     CDS             1..575
                     /gene="Moe"
                     /coded_by="XM_034329368.2:220..1947"
                     /db_xref="GeneID:117606647"
ORIGIN      
        1 mvagakmmnv rvttmdaele faiqqtttgk qlfdqvvkti glrevwffgl qytdnkgdlt
       61 wiklykkvmn qevkketplq fkfrakfype dvaeeliqdi tlrlfylqvk nailtdeiyc
      121 ppetsvllas yavqarhgdy qkgthtagfl mndrlvpqrv vdqhkmskee wessitnwwq
      181 ehrgmlreda mmeylkiaqd lemygvnyfe irnkkgtdlw lgvdalglni yekdnkltpk
      241 igfpwseirn isfndkkfii kpidkkapdf vffatrvkin krilalcmgn helymrrrkp
      301 dtidvqqmka qareekiakq qqreklqlei aareraekkq qeyeerlrnm aeemdrrqae
      361 lneaqemirr leeqlkqlqa akeeledrqk eltammekle lshemeaaer akleqeirak
      421 qeevqriqse veakdaearr lqeefeaakl rqeeadrayq anttphhhhv eendegeeeg
      481 edevphgdvt kdlatdesii dpveerrtla ernerlhdql kalkqdlaqs rdesketvmd
      541 kihrenvrqg rdkyktlrei rkgntkrrvd qfenm
//
LOCUS       XP_076545631             573 aa            linear   INV 03-SEP-2025
DEFINITION  moesin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545631
VERSION     XP_076545631.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689516.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="moesin isoform X3"
                     /calculated_mol_wt=67837
     Region          7..206
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          200..296
                     /region_name="FERM_C_ERM"
                     /note="FERM domain C-lobe/F3 of the ERM family; cd13194"
                     /db_xref="CDD:270015"
     Site            order(242..243,245..251,260,281,284..285,288)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(242,245..252,260,281,284..285,288)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(278,282)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270015"
     Site            281..296
                     /site_type="other"
                     /note="actin binding site 2 [polypeptide binding]"
                     /db_xref="CDD:270015"
     Region          330..447
                     /region_name="ERM_helical"
                     /note="Ezrin/radixin/moesin, alpha-helical domain;
                     pfam20492"
                     /db_xref="CDD:466641"
     Region          499..573
                     /region_name="ERM_C"
                     /note="Ezrin/radixin/moesin family C terminal; pfam00769"
                     /db_xref="CDD:459932"
     CDS             1..573
                     /gene="Moe"
                     /coded_by="XM_076689516.1:227..1948"
                     /db_xref="GeneID:117606647"
ORIGIN      
        1 mpktmnvrvt tmdaelefai qqtttgkqlf dqvvktiglr evwffglqyt dnkgdltwik
       61 lykkvmnqev kketplqfkf rakfypedva eeliqditlr lfylqvknai ltdeiycppe
      121 tsvllasyav qarhgdyqkg thtagflmnd rlvpqrvvdq hkmskeewes sitnwwqehr
      181 gmlredamme ylkiaqdlem ygvnyfeirn kkgtdlwlgv dalglniyek dnkltpkigf
      241 pwseirnisf ndkkfiikpi dkkapdfvff atrvkinkri lalcmgnhel ymrrrkpdti
      301 dvqqmkaqar eekiakqqqr eklqleiaar eraekkqqey eerlrnmaee mdrrqaelne
      361 aqemirrlee qlkqlqaake eledrqkelt ammeklelsh emeaaerakl eqeirakqee
      421 vqriqsevea kdaearrlqe efeaanrlrq eeadrayqan ttphhhhvee ndegeeeged
      481 evphgdvtkd latdesiidp veerrtlaer nerlhdqlka lkqdlaqsrd esketvmdki
      541 hrenvrqgrd kyktlreirk gntkrrvdqf enm
//
LOCUS       XP_034185260             572 aa            linear   INV 03-SEP-2025
DEFINITION  moesin isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185260
VERSION     XP_034185260.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329369.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="moesin isoform X4"
                     /calculated_mol_wt=67695
     Region          7..206
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          200..296
                     /region_name="FERM_C_ERM"
                     /note="FERM domain C-lobe/F3 of the ERM family; cd13194"
                     /db_xref="CDD:270015"
     Site            order(242..243,245..251,260,281,284..285,288)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(242,245..252,260,281,284..285,288)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(278,282)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270015"
     Site            281..296
                     /site_type="other"
                     /note="actin binding site 2 [polypeptide binding]"
                     /db_xref="CDD:270015"
     Region          330..449
                     /region_name="ERM_helical"
                     /note="Ezrin/radixin/moesin, alpha-helical domain;
                     pfam20492"
                     /db_xref="CDD:466641"
     Region          498..572
                     /region_name="ERM_C"
                     /note="Ezrin/radixin/moesin family C terminal; pfam00769"
                     /db_xref="CDD:459932"
     CDS             1..572
                     /gene="Moe"
                     /coded_by="XM_034329369.2:226..1944"
                     /db_xref="GeneID:117606647"
ORIGIN      
        1 mpktmnvrvt tmdaelefai qqtttgkqlf dqvvktiglr evwffglqyt dnkgdltwik
       61 lykkvmnqev kketplqfkf rakfypedva eeliqditlr lfylqvknai ltdeiycppe
      121 tsvllasyav qarhgdyqkg thtagflmnd rlvpqrvvdq hkmskeewes sitnwwqehr
      181 gmlredamme ylkiaqdlem ygvnyfeirn kkgtdlwlgv dalglniyek dnkltpkigf
      241 pwseirnisf ndkkfiikpi dkkapdfvff atrvkinkri lalcmgnhel ymrrrkpdti
      301 dvqqmkaqar eekiakqqqr eklqleiaar eraekkqqey eerlrnmaee mdrrqaelne
      361 aqemirrlee qlkqlqaake eledrqkelt ammeklelsh emeaaerakl eqeirakqee
      421 vqriqsevea kdaearrlqe efeaaklrqe eadrayqant tphhhhveen degeeegede
      481 vphgdvtkdl atdesiidpv eerrtlaern erlhdqlkal kqdlaqsrde sketvmdkih
      541 renvrqgrdk yktlreirkg ntkrrvdqfe nm
//
LOCUS       XP_034185225             557 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box protein N4 jumeau [Osmia lignaria lignaria].
ACCESSION   XP_034185225
VERSION     XP_034185225.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329334.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185225.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="forkhead box protein N4 jumeau"
                     /calculated_mol_wt=62272
     Region          <298..529
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          304..384
                     /region_name="FH_FOXN1-like"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     N1 (FOXN1) and similar proteins; cd20030"
                     /db_xref="CDD:410804"
     Site            order(309,327..328,331,350..351,353..354,357,364,376..378,
                     380)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410804"
     CDS             1..557
                     /gene="jumu"
                     /coded_by="XM_034329334.2:851..2524"
                     /db_xref="GeneID:117606633"
ORIGIN      
        1 mldtesrhgt glvqgptrdp wltmdyylgt dslslqemld vdikceiegv igghtelgfn
       61 ftdmsslemd ddpigcqgdi sgwfgstsli ngnsnssnsn fnldlsgsda asimvnpnsv
      121 mphiairsps pnngrrhfsf strkeikeek idvedelend agsyienene hendndnele
      181 neigndtete qyenditete edsedeqeis kpvtpsklkt vpvstlkasp qflktqtttk
      241 ingmpgirvr nfkllqnpnq tpqhvrkqiy nnnvhispga gamasmkrek efdlsdyddk
      301 sypkpaysys cliamalkns qtgslpvsei ynfmcehfpy fktapngwkn svrhnlslnk
      361 cfekiekpag ngnqrkgclw ainpakvakm deevqkwsrk dplaikkami ypdhleller
      421 gemkyagsgd vseetessgd eaveestafd esirghlaan svtdsydess qdcdvditeq
      481 lydeidieen kealhmqlni skqesfeyel spstkrqktl tgtiqgnyly qpvttsrrkt
      541 plllragmgs gsflkid
//
LOCUS       XP_034185224             557 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box protein N4 jumeau [Osmia lignaria lignaria].
ACCESSION   XP_034185224
VERSION     XP_034185224.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329333.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185224.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="forkhead box protein N4 jumeau"
                     /calculated_mol_wt=62272
     Region          <298..529
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          304..384
                     /region_name="FH_FOXN1-like"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     N1 (FOXN1) and similar proteins; cd20030"
                     /db_xref="CDD:410804"
     Site            order(309,327..328,331,350..351,353..354,357,364,376..378,
                     380)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410804"
     CDS             1..557
                     /gene="jumu"
                     /coded_by="XM_034329333.2:4747..6420"
                     /db_xref="GeneID:117606633"
ORIGIN      
        1 mldtesrhgt glvqgptrdp wltmdyylgt dslslqemld vdikceiegv igghtelgfn
       61 ftdmsslemd ddpigcqgdi sgwfgstsli ngnsnssnsn fnldlsgsda asimvnpnsv
      121 mphiairsps pnngrrhfsf strkeikeek idvedelend agsyienene hendndnele
      181 neigndtete qyenditete edsedeqeis kpvtpsklkt vpvstlkasp qflktqtttk
      241 ingmpgirvr nfkllqnpnq tpqhvrkqiy nnnvhispga gamasmkrek efdlsdyddk
      301 sypkpaysys cliamalkns qtgslpvsei ynfmcehfpy fktapngwkn svrhnlslnk
      361 cfekiekpag ngnqrkgclw ainpakvakm deevqkwsrk dplaikkami ypdhleller
      421 gemkyagsgd vseetessgd eaveestafd esirghlaan svtdsydess qdcdvditeq
      481 lydeidieen kealhmqlni skqesfeyel spstkrqktl tgtiqgnyly qpvttsrrkt
      541 plllragmgs gsflkid
//
LOCUS       XP_034185263             500 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606648 [Osmia lignaria lignaria].
ACCESSION   XP_034185263
VERSION     XP_034185263.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329372.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..500
                     /product="uncharacterized protein LOC117606648"
                     /calculated_mol_wt=59176
     Region          36..82
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(39..40,42..44,47..48,51,62..64,66,69)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(39,43,46..47,50..51,55,57..58,62..64,66)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     CDS             1..500
                     /gene="LOC117606648"
                     /coded_by="XM_034329372.2:723..2225"
                     /db_xref="GeneID:117606648"
ORIGIN      
        1 mnkgcwkfde edkalyidme dedteedklt kvyshwdqlp dilleeifsy ltmreryyas
       61 lvcrswyraf hlqnvwatft ledctltrgk fnyysgwqyv ldhlrtstcl nkvgknfrcl
      121 ifepmlnfyn lyefmnmisw yterqgsdnt svagvgtyir rlkftfpcnm anrddpdrir
      181 lfgtggklle alkrlmrnlr nlrclelidl mldskealhl mddicsnctq tlsklvlint
      241 tryycpllhv gvfinlnvlv vspqnlhedi idligytklq hlhivqnrys pkdttmrlpk
      301 rsswvkmrvn npyikvhfev esykptdill pidllehggi pchsivldnp nteisastil
      361 thgtffesti rvyafkgltr yflhrnfaqr ldealvqfcr mcpnlhtlmi rdkistatil
      421 eivstakalr clyvrrnvil krcdkdwvrl mnwspehyhw ikensrsyes tekevskilk
      481 yrwhmlseke fknqtvtlhl
//
LOCUS       XP_034185234             476 aa            linear   INV 03-SEP-2025
DEFINITION  SEC61 translocon subunit alpha [Osmia lignaria lignaria].
ACCESSION   XP_034185234
VERSION     XP_034185234.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329343.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="SEC61 translocon subunit alpha"
                     /calculated_mol_wt=52135
     Region          1..469
                     /region_name="PTZ00219"
                     /note="Sec61 alpha subunit; Provisional"
                     /db_xref="CDD:185519"
     CDS             1..476
                     /gene="Sec61alpha"
                     /coded_by="XM_034329343.2:152..1582"
                     /db_xref="GeneID:117606637"
ORIGIN      
        1 mgfkflevik pfcsilpeie kperkiqfre kvlwtaitlf iflvccqipl fgimssdsad
       61 pfywirvila snrgtlmelg ispivtsgli mqllsgtkii evgdtpkdra lfngaqklfg
      121 mvitvgqaiv yvmtgmygdp teigagvcll iiiqlfvagl ivllldellq kgyglgsgis
      181 lfiatnicet ivwkafspat vntgrgtefe gavialfhll atrqdkvral reafyrqnlp
      241 nlmnllatil vfaiviyfqg frvdlpiksa kyrgqyssyp iklfytsnip iilqsalvsn
      301 lyvisqmlav kfqgniivnl lgvwsdiggg gparsypvgg lcyylsppes vghilqdpih
      361 avlyilfmlg scaffskmwi evsgssakav akqlkdqqmv mrghrdnsmi helnryipta
      421 aafgglciga lsvladflga igsgtgilla vtiiyqyfei fvkeqsemgg mstllf
//
LOCUS       XP_034185264             441 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606649 [Osmia lignaria lignaria].
ACCESSION   XP_034185264
VERSION     XP_034185264.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329373.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="uncharacterized protein LOC117606649"
                     /calculated_mol_wt=50153
     Region          22..93
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); smart00868"
                     /db_xref="CDD:214871"
     Region          <259..432
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          268..288
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          296..316
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(301,303,305,307..308,311..312,315,329,331,335..336,
                     339..340,343,357,359,361,363..364,367..368,371)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          324..344
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          352..372
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          385..402
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          412..432
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..441
                     /gene="LOC117606649"
                     /coded_by="XM_034329373.2:154..1479"
                     /db_xref="GeneID:117606649"
ORIGIN      
        1 mktysrkkgd spsnviihvn elcrlclake eemvpiydde tiplslrima cvnlevyeed
       61 glpnmichpc kyqleksyqf kkkceaadak lrkhmkliqq ltgqseegek qdsnreelad
      121 ssnsgkskqv kqlladlvst kgdsgqadgd pievteeelv ggyilgmnse nlemeetmse
      181 dpenitlvpa eeraakahct irttrikqeq esedeaeevq raianadhkn vyvmeltsns
      241 sgqgeslklq pitntivepn qelkvfqcdk cpkaftrrim lkshqsvhst qrgftcqace
      301 kwfptrsalv rherthtgek pfgcnichra faqkeillrh lmthsgqkpf qcqhceksft
      361 qrealkvhmr rhqkldpndi qlhhcqlcpk afchasglsr hlvthtgrty kcveceksft
      421 dkssllrhsr ihapkkqilt n
//
LOCUS       XP_076545632             436 aa            linear   INV 03-SEP-2025
DEFINITION  interferon-related developmental regulator 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545632
VERSION     XP_076545632.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689517.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..436
                     /product="interferon-related developmental regulator 1"
                     /calculated_mol_wt=48282
     Region          32..336
                     /region_name="IFRD"
                     /note="Interferon-related developmental regulator (IFRD);
                     pfam05004"
                     /db_xref="CDD:461515"
     Region          381..431
                     /region_name="IFRD_C"
                     /note="Interferon-related protein conserved region;
                     pfam04836"
                     /db_xref="CDD:461452"
     CDS             1..436
                     /gene="LOC117606657"
                     /coded_by="XM_076689517.1:228..1538"
                     /db_xref="GeneID:117606657"
ORIGIN      
        1 mpgkgkrrgk sghggkrael tsdedsvnda csvtssqsdn rsmlddvndn evdeisqqea
       61 feeklkeaid gltqksakgr ticfngieki faikyvpdfv edrkmtitds ierglkkgrs
      121 eeqstaarls tllcvqlgaf esaetvcrdl ksiltfiasd ptastqarae ccwalsmnqf
      181 lsgndaadti eivqllsnif sgsylkgnga lanittevaa lhaaaisswt llltvmapad
      241 iynlltsnkt nsympslnkl sellesphld irlsageala vifelgrdfs sdyeqdwald
      301 ltevlkelat dsnkyrakkd rkqqranfrd ilryieedtv pemhvkfgqe vlylegwctr
      361 iqynaccrll gpginihlav nqllreifhl gnkvvplpln qktsklertl mnaaafkart
      421 iqrnknrdkr saalap
//
LOCUS       XP_034185265             404 aa            linear   INV 03-SEP-2025
DEFINITION  S-adenosylmethionine Synthetase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185265
VERSION     XP_034185265.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329374.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="S-adenosylmethionine Synthetase isoform X1"
                     /calculated_mol_wt=44528
     Region          27..399
                     /region_name="S-AdoMet_synt"
                     /note="S-adenosylmethionine synthetase; cd18079"
                     /db_xref="CDD:350837"
     Site            order(27..29,31,65,67,69,74,76..78,142..145,147,189,191,
                     193,210,212,251,254,265..267,269..272,275,280..287,289,
                     293,325,327..331,336..337,339)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350837"
     Site            order(31,37..39,63,78,121,124..125,141..142,187,189,214,
                     255,257..258,260,266,272..273,287..289,293,297,299,330)
                     /site_type="active"
                     /db_xref="CDD:350837"
     Site            order(31,37..38,187,189,273)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350837"
     CDS             1..404
                     /gene="Sam-S"
                     /coded_by="XM_034329374.2:599..1813"
                     /db_xref="GeneID:117606650"
ORIGIN      
        1 mpetahhmng yanghtppel qqdtsflfts esvgeghpdk mcdqisdail dahlqqdpda
       61 kvacetvtkt gmvllcgeit skavvdyqki vrdtvkhigy ddsskgfdwr tlnllvaieq
      121 qspniadgvh vdreekdvga gdqglmfgya tdetdecmpl tvvlahklnq kiaklrrsge
      181 lwwarpdskt qvtceyvmdh gacvpirvht vvvslqhsek igleelrkav mekvikevip
      241 arylddrtif hvnpcglfii ggpqsdaglt grkiivdtyg gwgahgggaf sgkdftkvdr
      301 saayaarwva kslvkaglcr rclvqvsyai gvaeplsitv fdygtsklsq nellnivnkn
      361 fdlrpgkivk elnlrnpiyq qtstyghfgr egftweqpka lild
//
LOCUS       XP_034185266             404 aa            linear   INV 03-SEP-2025
DEFINITION  S-adenosylmethionine Synthetase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185266
VERSION     XP_034185266.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329375.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="S-adenosylmethionine Synthetase isoform X2"
                     /calculated_mol_wt=44325
     Region          27..399
                     /region_name="S-AdoMet_synt"
                     /note="S-adenosylmethionine synthetase; cd18079"
                     /db_xref="CDD:350837"
     Site            order(27..29,31,65,67,69,74,76..78,142..145,147,189,191,
                     193,210,212,251,254,265..267,269..272,275,280..287,289,
                     293,325,327..331,336..337,339)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350837"
     Site            order(31,37..39,63,78,121,124..125,141..142,187,189,214,
                     255,257..258,260,266,272..273,287..289,293,297,299,330)
                     /site_type="active"
                     /db_xref="CDD:350837"
     Site            order(31,37..38,187,189,273)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350837"
     CDS             1..404
                     /gene="Sam-S"
                     /coded_by="XM_034329375.2:600..1814"
                     /db_xref="GeneID:117606650"
ORIGIN      
        1 mpetahhmng yanghtppel qqdtsflfts esvgeghpdk mcdqisdail dahlqqdpda
       61 kvacetvtkt gmvllcgeit skavvdyqki vrdtvkhigy ddsskgfdyk lcsvllalda
      121 qspniaagvh enrsdeevga gdqglmfgya tdetdecmpl tvvlahklnq kiaklrrsge
      181 lwwarpdskt qvtceyvmdh gacvpirvht vvvslqhsek igleelrkav mekvikevip
      241 arylddrtif hvnpcglfii ggpqsdaglt grkiivdtyg gwgahgggaf sgkdftkvdr
      301 saayaarwva kslvkaglcr rclvqvsyai gvaeplsitv fdygtsklsq nellnivnkn
      361 fdlrpgkivk elnlrnpiyq qtstyghfgr egftweqpka lild
//
LOCUS       XP_034185267             364 aa            linear   INV 03-SEP-2025
DEFINITION  S-adenosylmethionine Synthetase isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185267
VERSION     XP_034185267.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329376.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="S-adenosylmethionine Synthetase isoform X3"
                     /calculated_mol_wt=40150
     Region          1..359
                     /region_name="S-AdoMet_synt"
                     /note="S-adenosylmethionine synthetase; cd18079"
                     /db_xref="CDD:350837"
     Site            order(23,38,81,84..85,101..102,147,149,174,215,217..218,
                     220,226,232..233,247..249,253,257,259,290)
                     /site_type="active"
                     /db_xref="CDD:350837"
     Site            order(25,27,29,34,36..38,102..105,107,149,151,153,170,172,
                     211,214,225..227,229..232,235,240..247,249,253,285,
                     287..291,296..297,299)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350837"
     CDS             1..364
                     /gene="Sam-S"
                     /coded_by="XM_034329376.2:307..1401"
                     /db_xref="GeneID:117606650"
ORIGIN      
        1 mcdqisdail dahlqqdpda kvacetvtkt gmvllcgeit skavvdyqki vrdtvkhigy
       61 ddsskgfdwr tlnllvaieq qspniadgvh vdreekdvga gdqglmfgya tdetdecmpl
      121 tvvlahklnq kiaklrrsge lwwarpdskt qvtceyvmdh gacvpirvht vvvslqhsek
      181 igleelrkav mekvikevip arylddrtif hvnpcglfii ggpqsdaglt grkiivdtyg
      241 gwgahgggaf sgkdftkvdr saayaarwva kslvkaglcr rclvqvsyai gvaeplsitv
      301 fdygtsklsq nellnivnkn fdlrpgkivk elnlrnpiyq qtstyghfgr egftweqpka
      361 lild
//
LOCUS       XP_034185226             401 aa            linear   INV 03-SEP-2025
DEFINITION  aminoacylase-1 [Osmia lignaria lignaria].
ACCESSION   XP_034185226
VERSION     XP_034185226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329335.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..401
                     /product="aminoacylase-1"
                     /calculated_mol_wt=45188
     Region          10..399
                     /region_name="M20_AcylaseI_like"
                     /note="M20 Aminoacylase-I like subfamily; cd05646"
                     /db_xref="CDD:349898"
     CDS             1..401
                     /gene="LOC117606634"
                     /coded_by="XM_034329335.2:65..1270"
                     /db_xref="GeneID:117606634"
ORIGIN      
        1 msslskaqld atavenfrey lripsvqpni nyddciaflq kqaksldlpi kvyqvhpgkp
       61 ivvltwigtq psepaillns hmdvvpvfed mwtyppfgaq mdeqgniyar gsqdmkcvgi
      121 qyleairrfk stgqrfkrti hvsfvpdeei ggalgmkifv htkdfqalni gfaldegvac
      181 peeqfylfya ersiwhvave cqgtpghgsl lmdnsageki rtvidrfmdf rakekeklkn
      241 pelqlgdvts vnltqlrggv qtnvvppslt aifdirldps kdheefeami kkwceeagtd
      301 vtytfeqknp kiestrldds npywiafkkv cdnlginlqi gvfpggtdsr yvryvgipai
      361 gfspmnktki llhdhdeyln kdiflkgiei ylklipavan v
//
LOCUS       XP_034185268             346 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside hydrolase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185268
VERSION     XP_034185268.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329377.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185268.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="nucleoside hydrolase isoform X1"
                     /calculated_mol_wt=38029
     Region          44..341
                     /region_name="nuc_hydro"
                     /note="Nucleoside hydrolases. Nucleoside hydrolases cleave
                     the N-glycosidic bond in nucleosides generating ribose and
                     the respective base. These enzymes vary in their substrate
                     specificity. This group contains eukaryotic, bacterial and
                     archeal proteins similar...; cl00226"
                     /db_xref="CDD:469670"
     Site            order(50,54..55,81,167,202,208,210,279)
                     /site_type="active"
                     /db_xref="CDD:238257"
     CDS             1..346
                     /gene="LOC117606651"
                     /coded_by="XM_034329377.2:85..1125"
                     /db_xref="GeneID:117606651"
ORIGIN      
        1 maatmkvsmf ivtislvlfa isiyfiikei tiktftrsts avekliintd agaddamail
       61 ltlqsesdgf kvlaitcsyg nsyldnvvnn vlktltianr tdipvyigad rpliqvyrps
      121 myfgsdgfgd fnfskeitae rndskhaaia lidlvkqhpg eitllslapl tniattmtle
      181 peffkylknh vilggsvfgi gnvlpniefn fyqdpesnyi tlnrtktsvl lpwdtcvhss
      241 islgwrnnvl gkvnstimnf lneaerlsla kveswissdt maaavllrpe iatrtivtnv
      301 dpvlhgtarg svladftnlt grpentriil sfdvtafkel llnkls
//
LOCUS       XP_034185269             331 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside hydrolase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185269
VERSION     XP_034185269.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329378.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185269.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..331
                     /product="nucleoside hydrolase isoform X2"
                     /calculated_mol_wt=36230
     Region          29..326
                     /region_name="nuc_hydro"
                     /note="Nucleoside hydrolases. Nucleoside hydrolases cleave
                     the N-glycosidic bond in nucleosides generating ribose and
                     the respective base. These enzymes vary in their substrate
                     specificity. This group contains eukaryotic, bacterial and
                     archeal proteins similar...; cl00226"
                     /db_xref="CDD:469670"
     Site            order(35,39..40,66,152,187,193,195,264)
                     /site_type="active"
                     /db_xref="CDD:238257"
     CDS             1..331
                     /gene="LOC117606651"
                     /coded_by="XM_034329378.2:85..1080"
                     /db_xref="GeneID:117606651"
ORIGIN      
        1 maatmkvsmf ivtislvlfa irstsavekl iintdagadd amailltlqs esdgfkvlai
       61 tcsygnsyld nvvnnvlktl tianrtdipv yigadrpliq vyrpsmyfgs dgfgdfnfsk
      121 eitaerndsk haaialidlv kqhpgeitll slapltniat tmtlepeffk ylknhvilgg
      181 svfgignvlp niefnfyqdp esnyitlnrt ktsvllpwdt cvhssislgw rnnvlgkvns
      241 timnflneae rlslakvesw issdtmaaav llrpeiatrt ivtnvdpvlh gtargsvlad
      301 ftnltgrpen triilsfdvt afkelllnkl s
//
LOCUS       XP_034185270             331 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606652 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185270
VERSION     XP_034185270.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329379.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..331
                     /product="uncharacterized protein LOC117606652 isoform X1"
                     /calculated_mol_wt=36233
     Region          9..>70
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          <165..>255
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..331
                     /gene="LOC117606652"
                     /coded_by="XM_034329379.2:190..1185"
                     /db_xref="GeneID:117606652"
ORIGIN      
        1 mstqramfvn kqfnspinly spqaiqetld rqtqvlanga vgidfnqlak psnlqnsavl
       61 rmleeeearq ragqpslkrv awppppedqd fdfveqgpvq aktrgigera syqsspssgs
      121 spqppsiart saqsgapssp spvspggtlr qpshfqhqpv pkgwapvtpp atspvsqpay
      181 wphqqqqqqq rqppqqqnqr pqqqpqnsys prqtpiqapt afeappstit lrpeppisqa
      241 papvyqaqpa atkapvsgnm rgdlkwppps vkaqteaenr armelakgpa vrprrvrkdy
      301 tgffaqhalt ntypgyrapp gtqyftpayq h
//
LOCUS       XP_034185272             331 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606652 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185272
VERSION     XP_034185272.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329381.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..331
                     /product="uncharacterized protein LOC117606652 isoform X1"
                     /calculated_mol_wt=36233
     Region          9..>70
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          <165..>255
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..331
                     /gene="LOC117606652"
                     /coded_by="XM_034329381.2:53..1048"
                     /db_xref="GeneID:117606652"
ORIGIN      
        1 mstqramfvn kqfnspinly spqaiqetld rqtqvlanga vgidfnqlak psnlqnsavl
       61 rmleeeearq ragqpslkrv awppppedqd fdfveqgpvq aktrgigera syqsspssgs
      121 spqppsiart saqsgapssp spvspggtlr qpshfqhqpv pkgwapvtpp atspvsqpay
      181 wphqqqqqqq rqppqqqnqr pqqqpqnsys prqtpiqapt afeappstit lrpeppisqa
      241 papvyqaqpa atkapvsgnm rgdlkwppps vkaqteaenr armelakgpa vrprrvrkdy
      301 tgffaqhalt ntypgyrapp gtqyftpayq h
//
LOCUS       XP_034185273             331 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606652 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185273
VERSION     XP_034185273.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329382.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..331
                     /product="uncharacterized protein LOC117606652 isoform X1"
                     /calculated_mol_wt=36233
     Region          9..>70
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          <165..>255
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..331
                     /gene="LOC117606652"
                     /coded_by="XM_034329382.2:184..1179"
                     /db_xref="GeneID:117606652"
ORIGIN      
        1 mstqramfvn kqfnspinly spqaiqetld rqtqvlanga vgidfnqlak psnlqnsavl
       61 rmleeeearq ragqpslkrv awppppedqd fdfveqgpvq aktrgigera syqsspssgs
      121 spqppsiart saqsgapssp spvspggtlr qpshfqhqpv pkgwapvtpp atspvsqpay
      181 wphqqqqqqq rqppqqqnqr pqqqpqnsys prqtpiqapt afeappstit lrpeppisqa
      241 papvyqaqpa atkapvsgnm rgdlkwppps vkaqteaenr armelakgpa vrprrvrkdy
      301 tgffaqhalt ntypgyrapp gtqyftpayq h
//
LOCUS       XP_034185274             331 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606652 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185274
VERSION     XP_034185274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329383.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..331
                     /product="uncharacterized protein LOC117606652 isoform X1"
                     /calculated_mol_wt=36233
     Region          9..>70
                     /region_name="DUF4749"
                     /note="Domain of unknown function (DUF4749); pfam15936"
                     /db_xref="CDD:464948"
     Region          <165..>255
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..331
                     /gene="LOC117606652"
                     /coded_by="XM_034329383.2:187..1182"
                     /db_xref="GeneID:117606652"
ORIGIN      
        1 mstqramfvn kqfnspinly spqaiqetld rqtqvlanga vgidfnqlak psnlqnsavl
       61 rmleeeearq ragqpslkrv awppppedqd fdfveqgpvq aktrgigera syqsspssgs
      121 spqppsiart saqsgapssp spvspggtlr qpshfqhqpv pkgwapvtpp atspvsqpay
      181 wphqqqqqqq rqppqqqnqr pqqqpqnsys prqtpiqapt afeappstit lrpeppisqa
      241 papvyqaqpa atkapvsgnm rgdlkwppps vkaqteaenr armelakgpa vrprrvrkdy
      301 tgffaqhalt ntypgyrapp gtqyftpayq h
//
LOCUS       XP_034185275             295 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606652 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185275
VERSION     XP_034185275.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329384.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="uncharacterized protein LOC117606652 isoform X2"
                     /calculated_mol_wt=32284
     Region          <129..>219
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..295
                     /gene="LOC117606652"
                     /coded_by="XM_034329384.2:144..1031"
                     /db_xref="GeneID:117606652"
ORIGIN      
        1 mlaqtridfn qlakpsnlqn savlrmleee earqragqps lkrvawpppp edqdfdfveq
       61 gpvqaktrgi gerasyqssp ssgsspqpps iartsaqsga psspspvspg gtlrqpshfq
      121 hqpvpkgwap vtppatspvs qpaywphqqq qqqqrqppqq qnqrpqqqpq nsysprqtpi
      181 qaptafeapp stitlrpepp isqapapvyq aqpaatkapv sgnmrgdlkw pppsvkaqte
      241 aenrarmela kgpavrprrv rkdytgffaq haltntypgy rappgtqyft payqh
//
LOCUS       XP_034185276             265 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606652 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185276
VERSION     XP_034185276.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329385.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..265
                     /product="uncharacterized protein LOC117606652 isoform X3"
                     /calculated_mol_wt=28910
     Region          <99..>189
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..265
                     /gene="LOC117606652"
                     /coded_by="XM_034329385.2:16..813"
                     /db_xref="GeneID:117606652"
ORIGIN      
        1 mllvhealwg lkrvawpppp edqdfdfveq gpvqaktrgi gerasyqssp ssgsspqpps
       61 iartsaqsga psspspvspg gtlrqpshfq hqpvpkgwap vtppatspvs qpaywphqqq
      121 qqqqrqppqq qnqrpqqqpq nsysprqtpi qaptafeapp stitlrpepp isqapapvyq
      181 aqpaatkapv sgnmrgdlkw pppsvkaqte aenrarmela kgpavrprrv rkdytgffaq
      241 haltntypgy rappgtqyft payqh
//
LOCUS       XP_076545633             307 aa            linear   INV 03-SEP-2025
DEFINITION  27 kDa hemolymph protein-like isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545633
VERSION     XP_076545633.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689518.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="27 kDa hemolymph protein-like isoform X1"
                     /calculated_mol_wt=33671
     Region          64..276
                     /region_name="DUF1397"
                     /note="Protein of unknown function (DUF1397); pfam07165"
                     /db_xref="CDD:429328"
     CDS             1..307
                     /gene="LOC143305508"
                     /coded_by="XM_076689518.1:343..1266"
                     /db_xref="GeneID:143305508"
ORIGIN      
        1 mtkystltaa vflfgflght ysqenmptae evlhkvsniv dtsnlnisgn tdlntqlnss
       61 viqaidevkn vfkqkcdkng gpdaydnaqn akdrleqclk sfvniteleh emeinkpvgn
      121 ldmvfkkycq ktpvlkkcis dfttaiepcl epaerenkki vqnitdslln fvcykegdri
      181 alfisangpe clqskqqaiq nclnttfgsy appldfnngs apgldnlpsl ilgnkecndi
      241 sslqdcvvke lekcsdptpa nvvdsifrfi lketpcrnii ldksaastst vsllvittiv
      301 slllsla
//
LOCUS       XP_076545634             282 aa            linear   INV 03-SEP-2025
DEFINITION  27 kDa hemolymph protein-like isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545634
VERSION     XP_076545634.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689519.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="27 kDa hemolymph protein-like isoform X2"
                     /calculated_mol_wt=30861
     Region          39..251
                     /region_name="DUF1397"
                     /note="Protein of unknown function (DUF1397); pfam07165"
                     /db_xref="CDD:429328"
     CDS             1..282
                     /gene="LOC143305508"
                     /coded_by="XM_076689519.1:173..1021"
                     /db_xref="GeneID:143305508"
ORIGIN      
        1 mptaeevlhk vsnivdtsnl nisgntdlnt qlnssviqai devknvfkqk cdknggpday
       61 dnaqnakdrl eqclksfvni telehemein kpvgnldmvf kkycqktpvl kkcisdftta
      121 iepclepaer enkkivqnit dsllnfvcyk egdrialfis angpeclqsk qqaiqnclnt
      181 tfgsyappld fnngsapgld nlpslilgnk ecndisslqd cvvkelekcs dptpanvvds
      241 ifrfilketp crniildksa aststvsllv ittivsllls la
//
LOCUS       XP_034185277             305 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 35 member E3-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185277
VERSION     XP_034185277.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329386.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185277.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..305
                     /product="solute carrier family 35 member E3-like isoform
                     X1"
                     /calculated_mol_wt=35061
     Region          7..285
                     /region_name="TPT"
                     /note="Triose-phosphate Transporter family; cl26744"
                     /db_xref="CDD:474852"
     CDS             1..305
                     /gene="LOC117606653"
                     /coded_by="XM_034329386.2:187..1104"
                     /db_xref="GeneID:117606653"
ORIGIN      
        1 mkkkiltafy lilniffsiv ivllnkwlyi nvgfpnitls mihfvvtfig liicekfdif
       61 cikdiaikem flitvifcgf vvltnlsleh ntvgtyqvak mlttpcvimm qivfyrkqfs
      121 ifvkltlili tigvvinfyy diqfniigtv yasmgvflms lyqvminikq refqmdpmql
      181 lyyqaplsav mlffvvpfle pveqtltrsw slidlimvil sgivaflvnl tsywiigkts
      241 pltynmaghf kfcllllggs llfhetlnin qvigitltli giilyahvkv stmyllhyyl
      301 kktfh
//
LOCUS       XP_034185278             290 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 35 member E3-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185278
VERSION     XP_034185278.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329387.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185278.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..290
                     /product="solute carrier family 35 member E3-like isoform
                     X2"
                     /calculated_mol_wt=33148
     Region          7..285
                     /region_name="TPT"
                     /note="Triose-phosphate Transporter family; cl26744"
                     /db_xref="CDD:474852"
     CDS             1..290
                     /gene="LOC117606653"
                     /coded_by="XM_034329387.2:187..1059"
                     /db_xref="GeneID:117606653"
ORIGIN      
        1 mkkkiltafy lilniffsiv ivllnkwlyi nvgfpnitls mihfvvtfig liicekfdif
       61 cikdiaikem flitvifcgf vvltnlsleh ntvgtyqvak mlttpcvimm qivfyrkqfs
      121 ifvkltlili tigvvinfyy diqfniigtv yasmgvflms lyqvminikq refqmdpmql
      181 lyyqaplsav mlffvvpfle pveqtltrsw slidlimvil sgivaflvnl tsywiigkts
      241 pltynmaghf kfcllllggs llfhetlnin qvigitltli giilyahvki
//
LOCUS       XP_034185280             214 aa            linear   INV 03-SEP-2025
DEFINITION  calcyphosin-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034185280
VERSION     XP_034185280.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329389.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..214
                     /product="calcyphosin-like protein"
                     /calculated_mol_wt=24281
     Region          50..189
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..214
                     /gene="LOC117606655"
                     /coded_by="XM_034329389.2:168..812"
                     /db_xref="GeneID:117606655"
ORIGIN      
        1 mflhrpqsaa trhetelikk aqraihtvtd sieklrllcl argangiigl grafrrmded
       61 gnkklnleef ilgmretgle iseeeiqeif nrfdtdndgs vsvdefivai rppmsqsrkn
      121 vvdqafkkld ktgdgvittd dlrgvynvks hprymsgees eesilnkfla nfeqndttdg
      181 tvteeefmny ysaisasidh dayfdlmmrn aykl
//
LOCUS       XP_034185282             125 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606656 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185282
VERSION     XP_034185282.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329391.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="uncharacterized protein LOC117606656 isoform X1"
                     /calculated_mol_wt=14254
     CDS             1..125
                     /gene="LOC117606656"
                     /coded_by="XM_034329391.2:1..378"
                     /db_xref="GeneID:117606656"
ORIGIN      
        1 mnyydkcvfm yiigdwftge vqsvdtqnll dsviitnkti irrirvptye vykthwrndp
       61 eiqrhywaas fllpigtifv lalvvllmvl fkrcphivaa ivasilgvii iftalvsinh
      121 apiya
//
LOCUS       XP_034185283             117 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606656 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185283
VERSION     XP_034185283.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329392.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..117
                     /product="uncharacterized protein LOC117606656 isoform X2"
                     /calculated_mol_wt=13222
     CDS             1..117
                     /gene="LOC117606656"
                     /coded_by="XM_034329392.2:127..480"
                     /db_xref="GeneID:117606656"
ORIGIN      
        1 mfmgwgdwft gevqsvdtqn lldsviitnk tiirrirvpt yevykthwrn dpeiqrhywa
       61 asfllpigti fvlalvvllm vlfkrcphiv aaivasilgv iiiftalvsi nhapiya
//
LOCUS       XP_034185235              89 aa            linear   INV 03-SEP-2025
DEFINITION  protein anon-73B1 [Osmia lignaria lignaria].
ACCESSION   XP_034185235
VERSION     XP_034185235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329344.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="protein anon-73B1"
                     /calculated_mol_wt=9931
     Region          15..88
                     /region_name="UPF0239"
                     /note="Uncharacterized protein family (UPF0239);
                     pfam06783"
                     /db_xref="CDD:399634"
     CDS             1..89
                     /gene="LOC117606638"
                     /coded_by="XM_034329344.2:215..484"
                     /db_xref="GeneID:117606638"
ORIGIN      
        1 madadsqlkr lllpaeeslf dqllrfglyi gavfqiicll aivvyqagps dnvaalkddp
       61 sdvecsensp qvtprrphrp rkqekkkrr
//
LOCUS       XP_034185238              89 aa            linear   INV 03-SEP-2025
DEFINITION  protein anon-73B1 [Osmia lignaria lignaria].
ACCESSION   XP_034185238
VERSION     XP_034185238.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329347.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="protein anon-73B1"
                     /calculated_mol_wt=9931
     Region          15..88
                     /region_name="UPF0239"
                     /note="Uncharacterized protein family (UPF0239);
                     pfam06783"
                     /db_xref="CDD:399634"
     CDS             1..89
                     /gene="LOC117606638"
                     /coded_by="XM_034329347.2:97..366"
                     /db_xref="GeneID:117606638"
ORIGIN      
        1 madadsqlkr lllpaeeslf dqllrfglyi gavfqiicll aivvyqagps dnvaalkddp
       61 sdvecsensp qvtprrphrp rkqekkkrr
//
LOCUS       XP_034185237              89 aa            linear   INV 03-SEP-2025
DEFINITION  protein anon-73B1 [Osmia lignaria lignaria].
ACCESSION   XP_034185237
VERSION     XP_034185237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329346.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="protein anon-73B1"
                     /calculated_mol_wt=9931
     Region          15..88
                     /region_name="UPF0239"
                     /note="Uncharacterized protein family (UPF0239);
                     pfam06783"
                     /db_xref="CDD:399634"
     CDS             1..89
                     /gene="LOC117606638"
                     /coded_by="XM_034329346.2:278..547"
                     /db_xref="GeneID:117606638"
ORIGIN      
        1 madadsqlkr lllpaeeslf dqllrfglyi gavfqiicll aivvyqagps dnvaalkddp
       61 sdvecsensp qvtprrphrp rkqekkkrr
//
LOCUS       XP_034185239              89 aa            linear   INV 03-SEP-2025
DEFINITION  protein anon-73B1 [Osmia lignaria lignaria].
ACCESSION   XP_034185239
VERSION     XP_034185239.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329348.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="protein anon-73B1"
                     /calculated_mol_wt=9931
     Region          15..88
                     /region_name="UPF0239"
                     /note="Uncharacterized protein family (UPF0239);
                     pfam06783"
                     /db_xref="CDD:399634"
     CDS             1..89
                     /gene="LOC117606638"
                     /coded_by="XM_034329348.2:82..351"
                     /db_xref="GeneID:117606638"
ORIGIN      
        1 madadsqlkr lllpaeeslf dqllrfglyi gavfqiicll aivvyqagps dnvaalkddp
       61 sdvecsensp qvtprrphrp rkqekkkrr
//
LOCUS       XP_034185240              89 aa            linear   INV 03-SEP-2025
DEFINITION  protein anon-73B1 [Osmia lignaria lignaria].
ACCESSION   XP_034185240
VERSION     XP_034185240.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329349.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="protein anon-73B1"
                     /calculated_mol_wt=9931
     Region          15..88
                     /region_name="UPF0239"
                     /note="Uncharacterized protein family (UPF0239);
                     pfam06783"
                     /db_xref="CDD:399634"
     CDS             1..89
                     /gene="LOC117606638"
                     /coded_by="XM_034329349.2:24..293"
                     /db_xref="GeneID:117606638"
ORIGIN      
        1 madadsqlkr lllpaeeslf dqllrfglyi gavfqiicll aivvyqagps dnvaalkddp
       61 sdvecsensp qvtprrphrp rkqekkkrr
//
LOCUS       XP_034184891             597 aa            linear   INV 03-SEP-2025
DEFINITION  choline transporter [Osmia lignaria lignaria].
ACCESSION   XP_034184891
VERSION     XP_034184891.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329000.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="choline transporter"
                     /calculated_mol_wt=64379
     Region          7..481
                     /region_name="SLC5sbd_CHT"
                     /note="Na(+)- and Cl(-)-dependent choline cotransporter
                     CHT and related proteins; solute-binding domain; cd11474"
                     /db_xref="CDD:271368"
     Site            order(56,59,331,334..335)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271368"
     CDS             1..597
                     /gene="ChT"
                     /coded_by="XM_034329000.2:699..2492"
                     /db_xref="GeneID:117606498"
ORIGIN      
        1 minlaglvsi ilfyvlilav giwaarkkea gnnseeevml agrsigmfvg iftmtatwvg
       61 ggyingtaea iytrglvwcq apfgyalslv fggiffankm reqgyitmld plqdafgerm
      121 ggllflpalc gevfwaagil aalgatlavi idmdqgysvi lsaciaifyt lfgglysvay
      181 tdviqlfcif iglwmcipfa wanpkvesls smdvdwigev dskeywsyvd nglllifggi
      241 pwqvyfqrvl ssksatraqi lsyvaaigci lmaippvlig aiakatpwne tdykgpyplt
      301 vdetsmilpm vlqyltpdfv sffglgavsa avmssadssi lsassmfarn vyklifrqra
      361 sekeiiwvmr lgigvvgvls tvmaltipsi yglwsmcsdl vyvilfpqll mvvhfkdncn
      421 tygslsayii aflvrvsgge pllglpalih ypgydeetkt qtfpfrtmam llslvtligv
      481 sygtqvaflt grvapgydvf rcvvnipedv ervgpdpaeg eqmavlaagv grlygskdes
      541 ngrvnpalep dydmepgyka pgmmegvvgg gggggagahl vphgpnvprq pqsstef
//
LOCUS       XP_034184892             597 aa            linear   INV 03-SEP-2025
DEFINITION  choline transporter [Osmia lignaria lignaria].
ACCESSION   XP_034184892
VERSION     XP_034184892.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329001.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="choline transporter"
                     /calculated_mol_wt=64379
     Region          7..481
                     /region_name="SLC5sbd_CHT"
                     /note="Na(+)- and Cl(-)-dependent choline cotransporter
                     CHT and related proteins; solute-binding domain; cd11474"
                     /db_xref="CDD:271368"
     Site            order(56,59,331,334..335)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271368"
     CDS             1..597
                     /gene="ChT"
                     /coded_by="XM_034329001.2:366..2159"
                     /db_xref="GeneID:117606498"
ORIGIN      
        1 minlaglvsi ilfyvlilav giwaarkkea gnnseeevml agrsigmfvg iftmtatwvg
       61 ggyingtaea iytrglvwcq apfgyalslv fggiffankm reqgyitmld plqdafgerm
      121 ggllflpalc gevfwaagil aalgatlavi idmdqgysvi lsaciaifyt lfgglysvay
      181 tdviqlfcif iglwmcipfa wanpkvesls smdvdwigev dskeywsyvd nglllifggi
      241 pwqvyfqrvl ssksatraqi lsyvaaigci lmaippvlig aiakatpwne tdykgpyplt
      301 vdetsmilpm vlqyltpdfv sffglgavsa avmssadssi lsassmfarn vyklifrqra
      361 sekeiiwvmr lgigvvgvls tvmaltipsi yglwsmcsdl vyvilfpqll mvvhfkdncn
      421 tygslsayii aflvrvsgge pllglpalih ypgydeetkt qtfpfrtmam llslvtligv
      481 sygtqvaflt grvapgydvf rcvvnipedv ervgpdpaeg eqmavlaagv grlygskdes
      541 ngrvnpalep dydmepgyka pgmmegvvgg gggggagahl vphgpnvprq pqsstef
//
LOCUS       XP_034184893             316 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606499 [Osmia lignaria lignaria].
ACCESSION   XP_034184893
VERSION     XP_034184893.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329002.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="uncharacterized protein LOC117606499"
                     /calculated_mol_wt=27027
     CDS             1..316
                     /gene="LOC117606499"
                     /coded_by="XM_034329002.2:255..1205"
                     /db_xref="GeneID:117606499"
ORIGIN      
        1 mrpakswatl aaavlttalf wqsisadapi sgsylppsts ygtpnigggs ggnggsfgap
       61 sssygapngg gggggggggg rpsstygaps snygapsssy gapssgngap sssygapssg
      121 ggggfggggg fgggfgggis ssygapsrgg ggggslsssy gapssggggl sssygaptgg
      181 gggysrpsss ygtpstgggs fngggrgysg ggggysnggg grggysgggg gysgggggys
      241 nggggysngg gysggggggy sggggggysg ggggysgggg gysggggggy sggggggysg
      301 gggggqgyas nggyqy
//
LOCUS       XP_034185345            1366 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606677 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185345
VERSION     XP_034185345.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329454.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185345.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1366
                     /product="uncharacterized protein LOC117606677 isoform X1"
                     /calculated_mol_wt=152176
     Region          539..906
                     /region_name="Periplasmic_Binding_Protein_Type_2"
                     /note="Type 2 periplasmic binding fold superfamily;
                     cl21456"
                     /db_xref="CDD:473866"
     Region          679..948
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..1366
                     /gene="LOC117606677"
                     /coded_by="XM_034329454.2:391..4491"
                     /db_xref="GeneID:117606677"
ORIGIN      
        1 mkrrnretmk mfgfpckfif lflsailfcs vascsadaar saravkgrlr tsynawrvvl
       61 clsqsqgslk rdvqkaweka rlesshadmn vsyieappkt dllpnplsll nkfcgeikgg
      121 ktvlsliigg gsaarflvta aaslnipalw lpfthedflr qgnlgkyesr igsstkevga
      181 aaaalmhran whaftllidt tllplthllh anqttltprk iihlptndkt lkmrlrrvae
      241 eggsggvivm gcdlnnarki lgvagkyeml egrflwlwld lkaelrpnep nvisshilhs
      301 srssiatnen papvesisrt anlvpesqls lnalpsland ihrlqeyrwq neksvgkqdd
      361 kvfnfdddee irlmnkdlns kdfmpvgmla lrpsgiklmg sdtilsrmir etsqaldetf
      421 vevkskvnym seahlddnfv pecfpernfk flnseirrnv skilaqklrk avsqiskdka
      481 kfhllnlqai rfsgnktqlr wtkvgtvkgg kevhldtiiw pgggivpayl eqggekigmp
      541 myrivtalas pftmvtslqe giclrglfcr qgdtvmccyg ltmdlmslvs relgfrydly
      601 lvkdglfgkr ngrtgtwngi mgelvsgraq lafaplsvsa rraevvdftt pyffsgvsfl
      661 tapkpnyeip lfaflfpfst elwiavftsl nftaiavaly ewfspfglnp wgrqrsknfs
      721 iasalwvmwg llcghlvafk apkswpnkfl iniwggfsvi fvasytania aliaglffhs
      781 agsnydksll lqkvgapras aaeyyvqkan pdlwphmary slsnvaegve rlrngsldil
      841 iadtpildyy ratddgcrlq kigdtinedt yavaltkghp lkesiskvia nytstglldi
      901 lqekwygglp cirgrkgmda glmheqrgqp rplgvasvag vfcllgmgvv lgtiilageh
      961 lfykytlprl rhrpedsiwr srnvmffsqk lyrfincvel vsphhaarel vhtvrqgqia
     1021 slfqksvkrk eheqrrrrks kaqffemiqe irrvqqedki etvfevterm ttfkkeekav
     1081 kerersrsks plmprspkrs eksrsstnls ssrlglspis leapmkpref tlsstnlrar
     1141 spletvgrrl shgdggsppp rlgshfggsa tlrpfatarn dtatsgtaat lkaepsvgga
     1201 ptpryarspa krgqsfpvfa tlrpphssgy qiskspllsp neltstigrk lsrewgsgtv
     1261 dlarstetva kgstytlsqe mtlslerpsh ekiqddampv kkpirrarsh enretgklma
     1321 dlpsprlaaq pvvggrsvse rtkkqlesel kailsarahh rdlhpp
//
LOCUS       XP_034185346            1365 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606677 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185346
VERSION     XP_034185346.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329455.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185346.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1365
                     /product="uncharacterized protein LOC117606677 isoform X2"
                     /calculated_mol_wt=152048
     Region          539..906
                     /region_name="Periplasmic_Binding_Protein_Type_2"
                     /note="Type 2 periplasmic binding fold superfamily;
                     cl21456"
                     /db_xref="CDD:473866"
     Region          679..948
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..1365
                     /gene="LOC117606677"
                     /coded_by="XM_034329455.2:391..4488"
                     /db_xref="GeneID:117606677"
ORIGIN      
        1 mkrrnretmk mfgfpckfif lflsailfcs vascsadaar saravkgrlr tsynawrvvl
       61 clsqsqgslk rdvqkaweka rlesshadmn vsyieappkt dllpnplsll nkfcgeikgg
      121 ktvlsliigg gsaarflvta aaslnipalw lpfthedflr qgnlgkyesr igsstkevga
      181 aaaalmhran whaftllidt tllplthllh anqttltprk iihlptndkt lkmrlrrvae
      241 eggsggvivm gcdlnnarki lgvagkyeml egrflwlwld lkaelrpnep nvisshilhs
      301 srssiatnen papvesisrt anlvpesqls lnalpsland ihrlqeyrwq neksvgkqdd
      361 kvfnfdddee irlmnkdlns kdfmpvgmla lrpsgiklmg sdtilsrmir etsqaldetf
      421 vevkskvnym seahlddnfv pecfpernfk flnseirrnv skilaqklrk avsqiskdka
      481 kfhllnlqai rfsgnktqlr wtkvgtvkgg kevhldtiiw pgggivpayl eqggekigmp
      541 myrivtalas pftmvtslqe giclrglfcr qgdtvmccyg ltmdlmslvs relgfrydly
      601 lvkdglfgkr ngrtgtwngi mgelvsgraq lafaplsvsa rraevvdftt pyffsgvsfl
      661 tapkpnyeip lfaflfpfst elwiavftsl nftaiavaly ewfspfglnp wgrqrsknfs
      721 iasalwvmwg llcghlvafk apkswpnkfl iniwggfsvi fvasytania aliaglffhs
      781 agsnydksll lqkvgapras aaeyyvqkan pdlwphmary slsnvaegve rlrngsldil
      841 iadtpildyy ratddgcrlq kigdtinedt yavaltkghp lkesiskvia nytstglldi
      901 lqekwygglp cirgrkgmda glmheqrgqp rplgvasvag vfcllgmgvv lgtiilageh
      961 lfykytlprl rhrpedsiwr srnvmffsqk lyrfincvel vsphhaarel vhtvrqgqia
     1021 slfqksvkre heqrrrrksk aqffemiqei rrvqqedkie tvfevtermt tfkkeekavk
     1081 erersrsksp lmprspkrse ksrsstnlss srlglspisl eapmkpreft lsstnlrars
     1141 pletvgrrls hgdggspppr lgshfggsat lrpfatarnd tatsgtaatl kaepsvggap
     1201 tpryarspak rgqsfpvfat lrpphssgyq iskspllspn eltstigrkl srewgsgtvd
     1261 larstetvak gstytlsqem tlslerpshe kiqddampvk kpirrarshe nretgklmad
     1321 lpsprlaaqp vvggrsvser tkkqleselk ailsarahhr dlhpp
//
LOCUS       XP_034185348            1344 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606677 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185348
VERSION     XP_034185348.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329457.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185348.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1344
                     /product="uncharacterized protein LOC117606677 isoform X3"
                     /calculated_mol_wt=149628
     Region          517..884
                     /region_name="Periplasmic_Binding_Protein_Type_2"
                     /note="Type 2 periplasmic binding fold superfamily;
                     cl21456"
                     /db_xref="CDD:473866"
     Region          657..926
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..1344
                     /gene="LOC117606677"
                     /coded_by="XM_034329457.2:391..4425"
                     /db_xref="GeneID:117606677"
ORIGIN      
        1 mkrrnretmk mfgfpckfif lflsailfcs vascsadaar saravkgrlr tsynawrvvl
       61 clsqsqgslk rdvqkaweka rlesshadmn vsyieappkt dllpnplsll nkfcgeikgg
      121 ktvlsliigg gsaarflvta aaslnipalw lpfthedflr qgnlgkyesr igsstkevga
      181 aaaalmhran whaftllidt tllplthllh anqttltprk iihlptndkt lkmrlrrvae
      241 eggsggvivm gcdlnnarki lgvagkyeml egrflwlcsr ssiatnenpa pvesisrtan
      301 lvpesqlsln alpslandih rlqeyrwqne ksvgkqddkv fnfdddeeir lmnkdlnskd
      361 fmpvgmlalr psgiklmgsd tilsrmiret sqaldetfve vkskvnymse ahlddnfvpe
      421 cfpernfkfl nseirrnvsk ilaqklrkav sqiskdkakf hllnlqairf sgnktqlrwt
      481 kvgtvkggke vhldtiiwpg ggivpayleq ggekigmpmy rivtalaspf tmvtslqegi
      541 clrglfcrqg dtvmccyglt mdlmslvsre lgfrydlylv kdglfgkrng rtgtwngimg
      601 elvsgraqla faplsvsarr aevvdfttpy ffsgvsflta pkpnyeiplf aflfpfstel
      661 wiavftslnf taiavalyew fspfglnpwg rqrsknfsia salwvmwgll cghlvafkap
      721 kswpnkflin iwggfsvifv asytaniaal iaglffhsag snydkslllq kvgaprasaa
      781 eyyvqkanpd lwphmarysl snvaegverl rngsldilia dtpildyyra tddgcrlqki
      841 gdtinedtya valtkghplk esiskviany tstglldilq ekwygglpci rgrkgmdagl
      901 mheqrgqprp lgvasvagvf cllgmgvvlg tiilagehlf ykytlprlrh rpedsiwrsr
      961 nvmffsqkly rfincvelvs phhaarelvh tvrqgqiasl fqksvkrkeh eqrrrrkska
     1021 qffemiqeir rvqqedkiet vfevtermtt fkkeekavke rersrskspl mprspkrsek
     1081 srsstnlsss rlglspisle apmkpreftl sstnlrarsp letvgrrlsh gdggsppprl
     1141 gshfggsatl rpfatarndt atsgtaatlk aepsvggapt pryarspakr gqsfpvfatl
     1201 rpphssgyqi skspllspne ltstigrkls rewgsgtvdl arstetvakg stytlsqemt
     1261 lslerpshek iqddampvkk pirrarshen retgklmadl psprlaaqpv vggrsvsert
     1321 kkqleselka ilsarahhrd lhpp
//
LOCUS       XP_034184894             877 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-14 [Osmia lignaria lignaria].
ACCESSION   XP_034184894
VERSION     XP_034184894.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..877
                     /product="sorting nexin-14"
                     /calculated_mol_wt=99504
     Region          126..273
                     /region_name="PXA"
                     /note="PXA domain; pfam02194"
                     /db_xref="CDD:460484"
     Region          319..445
                     /region_name="RGS"
                     /note="Regulator of G protein signaling (RGS) domain
                     superfamily; cl02565"
                     /db_xref="CDD:470619"
     Region          526..628
                     /region_name="PX_domain"
                     /note="The Phox Homology domain, a phosphoinositide
                     binding module; cl02563"
                     /db_xref="CDD:470617"
     Site            order(562..564,590..591,600)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132768"
     CDS             1..877
                     /gene="LOC117606502"
                     /coded_by="XM_034329003.2:303..2936"
                     /db_xref="GeneID:117606502"
ORIGIN      
        1 mvlssrdrvq dfyvcnviip vfvfllvlft livfksiiwa lvicclyvin ifyglvilqy
       61 inlvlvnlna isdtfnptfe vkdpcsvcsn nsckrhkqlp hsakvkipkd cdraleqlle
      121 dllqkyvcaw ysdfsadeaf vqqlrlatat aakniavrll rtdvvnvvlc rlvplilqha
      181 qdwknlnaki ndtsrlgdqi inyfgheihp asysrkaeln ylrgvvtaii phllpaihvs
      241 tnnkvilrei lanwillpav daiadpenin alvmlsthrd glvsnsmdav nvpmlqywvt
      301 issmpkiaqn ylkpsldevl nnpqllymfm qhiketgpmn lfqfcldidd lskrmlnpem
      361 ssnaedslyl diqsmyltyl dpegpeylhl pedismgirr ilersskkiq elrtsrpfyq
      421 ahqeahalle stclpsfhhs yqlydllcgq papnpvkqsr asvssgtgns sskisnhfpk
      481 idgvlrttti dgmpfqdiyq geeidyssra yneikgaddt fsrdlttwrv niphmetgdn
      541 qalyvidvhn vaegkswtvl rrdqdfyvlr trlaefhgdk elsdsplpsr knphgsytin
      601 rqryedflek llskpvlrss ellynfltmp nfkpyltncs tpdigilyqs manklrkekg
      661 qnldkfmnvf lastnvkney mdvgveplge psvneskkvk grdllfgvfg dnlnfdpnir
      721 dlsssssler shvkgasfct vdavdrllnv ppiitrilwm fasssrakmd pyvnvllynt
      781 lakllsggra aivvkllhak vmgdksstkd tsrrnyyena kkglygllpc wligfykpwt
      841 elmdsildsl qnaalnkhla yvlldhilin lfpeltv
//
LOCUS       XP_034184895             489 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial calcium uptake 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184895
VERSION     XP_034184895.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329004.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184895.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="mitochondrial calcium uptake 1 isoform X1"
                     /calculated_mol_wt=57210
     Region          233..453
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cd15900"
                     /db_xref="CDD:320080"
     Region          233..262
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Site            order(242,244,246,253,433,435,437,444)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320080"
     Region          287..317
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          387..416
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          424..453
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     CDS             1..489
                     /gene="MICU1"
                     /coded_by="XM_034329004.2:205..1674"
                     /db_xref="GeneID:117606503"
ORIGIN      
        1 mfinkvihii rrnvscetik ksnilllrdy tnnfrysskr nfryfsyeqk lkrnrillll
       61 fipcfvtsli gwksfsikgr yfpfvdaaeq iteeesnded kqieeqqdne kkkkkekigf
      121 rdrkiieyen rlraystpdk ifryfatvkv shldytevym tpddfvrsit pgmrqpdglg
      181 ldkyrrydpk hihtklelal dedsifyklg saglitfsdy iflltvlsts rrhfeiafrm
      241 fdfngdgdvd seefgkvatl irqqtsignr hrdhvatgnm fkgvnsaltt yffgsnmngk
      301 ltiekfldfq qqlqkeilsl eferrhpden grisevdfte lllayagypd kkktkiiktv
      361 kkrfkddskg idkdeylkff hflnnindvd taltfyhiag asidqatlkh vaktvahvdl
      421 sdhviqvvyt ifdenldgql snkefvavmk nrvlrglekp kdtgfvklie slakclkity
      481 nmhfenynk
//
LOCUS       XP_034184896             489 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial calcium uptake 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184896
VERSION     XP_034184896.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329005.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184896.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="mitochondrial calcium uptake 1 isoform X2"
                     /calculated_mol_wt=57182
     Region          233..453
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cd15900"
                     /db_xref="CDD:320080"
     Region          233..262
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Site            order(242,244,246,253,433,435,437,444)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320080"
     Region          287..317
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          387..416
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          424..453
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     CDS             1..489
                     /gene="MICU1"
                     /coded_by="XM_034329005.2:205..1674"
                     /db_xref="GeneID:117606503"
ORIGIN      
        1 mfinkvihii rrnvscetik ksnilllrdy tnnfrysskr nfryfsyeqk lkrnrillll
       61 fipcfvtsli gwksfsikgr yfpfvdaaeq iteeesnded kqieeqqdne kkkkkekigf
      121 rdrkiieyen rmrhystpdk vfryfatlqv vnndipeifm tpddflrsit pgvkqpdglg
      181 ldkyrrydpk hihtklelal dedsifyklg saglitfsdy iflltvlsts rrhfeiafrm
      241 fdfngdgdvd seefgkvatl irqqtsignr hrdhvatgnm fkgvnsaltt yffgsnmngk
      301 ltiekfldfq qqlqkeilsl eferrhpden grisevdfte lllayagypd kkktkiiktv
      361 kkrfkddskg idkdeylkff hflnnindvd taltfyhiag asidqatlkh vaktvahvdl
      421 sdhviqvvyt ifdenldgql snkefvavmk nrvlrglekp kdtgfvklie slakclkity
      481 nmhfenynk
//
LOCUS       XP_076545636              58 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606620 [Osmia lignaria lignaria].
ACCESSION   XP_076545636
VERSION     XP_076545636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689521.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..58
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..58
                     /product="uncharacterized protein LOC117606620"
                     /calculated_mol_wt=7019
     CDS             1..58
                     /gene="LOC117606620"
                     /coded_by="XM_076689521.1:230..406"
                     /db_xref="GeneID:117606620"
ORIGIN      
        1 mehqknnklq fgyvcenpmq weqrfeekdl aenrhrgkvk wgnidgsyge hywdlnhk
//
LOCUS       XP_034190275             561 aa            linear   INV 03-SEP-2025
DEFINITION  dnaJ homolog subfamily C member 11 [Osmia lignaria lignaria].
ACCESSION   XP_034190275
VERSION     XP_034190275.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334384.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="dnaJ homolog subfamily C member 11"
                     /calculated_mol_wt=63793
     Region          14..79
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(42..44,56,59..60,63..64)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          424..552
                     /region_name="DnaJ-like_C11_C"
                     /note="DnaJ-like protein C11, C-terminal; pfam11875"
                     /db_xref="CDD:463378"
     CDS             1..561
                     /gene="LOC117608785"
                     /coded_by="XM_034334384.2:287..1972"
                     /db_xref="GeneID:117608785"
ORIGIN      
        1 mdedndkehl leddyytfln iprnatqeei nnayrrqskl yhpdkhvdpv hkkeaevlfn
       61 rtkeaykvls nphqraiyds vgirglqtdg weivertktp qeireeyehl akeaeerklm
      121 qltnpttsiv mninatdlfd ryddddyeds rnilsclevs gmsftqsiea pltlqdtvtl
      181 ygqvttqngi gsgsvnfaar rllsskgwve vdfgagngpm lgfkgfrvlp hklmlnggti
      241 leftpvemka nlvgtlsmel dthtigqlty nagpesamst aivrdtsrsy tsfrlqfgiq
      301 rsflslnyth kmeergmklr ggvkvgtfgv tveygaekki srhskifatv cagvptgval
      361 kiklkrsfqt ysfpiqlsde llpatvfyat vvplvtwvfv kkmvidpmvk krmerekeke
      421 kevnktrmme kqkeaesatk lmsatvsrir aqeeakkgli itkalygrfv ypqeeqynse
      481 qpmrrdevid vtiplqclvk dsklilydas kselpgfydp cvgeekqlli qylfrsrthe
      541 civkdnapvr iplpshrvnt t
//
LOCUS       XP_034190276             162 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608786 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190276
VERSION     XP_034190276.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334385.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..162
                     /product="uncharacterized protein LOC117608786 isoform X1"
                     /calculated_mol_wt=18615
     CDS             1..162
                     /gene="LOC117608786"
                     /coded_by="XM_034334385.2:240..728"
                     /db_xref="GeneID:117608786"
ORIGIN      
        1 mrvllkcspt hstsyksyye vrtpylppfi ppsvienisk rskrcklpdt splshelfdh
       61 etqamrlaad gkspiwyclq vmnyyqfeke pkprftysrd vdtdlitasv rlssgnifig
      121 dpcedyekal gsaakkayee deelssfyrk hfestlsttf rs
//
LOCUS       XP_034190277             147 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608786 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190277
VERSION     XP_034190277.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334386.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..147
                     /product="uncharacterized protein LOC117608786 isoform X2"
                     /calculated_mol_wt=16714
     CDS             1..147
                     /gene="LOC117608786"
                     /coded_by="XM_034334386.2:241..684"
                     /db_xref="GeneID:117608786"
ORIGIN      
        1 mrvllkcspt hstsyksyye vrtpylppfi ppsvienisk rskrcklpdt splshelfdh
       61 etqamrlaad gkspiwyclq vmnyyqfeke pkprftysrd vdtdlitasv rlssgnifig
      121 dpcedyekal gsaakkayet clfsigr
//
LOCUS       XP_034190337            1636 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608817 [Osmia lignaria lignaria].
ACCESSION   XP_034190337
VERSION     XP_034190337.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334446.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190337.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1636
                     /product="uncharacterized protein LOC117608817"
                     /calculated_mol_wt=182083
     Region          1318..1632
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(1320..1323,1326,1328,1341,1343,1376,1392..1395,1438,
                     1442..1443,1445,1455..1456)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1636
                     /gene="LOC117608817"
                     /coded_by="XM_034334446.2:60..4970"
                     /db_xref="GeneID:117608817"
ORIGIN      
        1 mpyygdglpy yyggafsnhs slmtggpraf htplsrfsph lstisespls tlrryspvsv
       61 ptrprrvidt adidvstpri hhqdanrprn rlrrdrptik irsqalkdnp alrehnerhe
      121 ktvgellvek flikdkklee aerrmqlfhq isldrdeede elqqinnkit rrftrrrssa
      181 diqldpeqle revayaqvqa kvldtlvaee qaeienevrr gtlikksnag gprtvpgffr
      241 nvdgdsasqg eeeaaqkmrk tlkklkkkkk ttekpsppdd deddsrvrrs stssdvsvel
      301 etdekearkh kkplmfkvea snsvgdfsti lvnsggadna vveqfresvk ipvprrvgir
      361 ktdrdsvdee setevvlpvk rpyvkdtsrn svhftvktpp envkndplrk irpvgnveee
      421 lqsvgskeen keetespkie tpkilgvsgl kreesqqssk evpespkspa tprlkkkeie
      481 cfkvnsteve skeapqspkf pvtsilkket pkspviskfn ktgplekktp gspkspdasm
      541 lkkeespmtg kpastmvdet svvlgvdpla nensasaalp svadrlpkas kintdennav
      601 eapkrslpea eylakptksv rdedvgyplp gkkankaptl knasdsaasg apvrenqvkr
      661 psdreerlgk tvtddaigkk lvtkttlfeg pksdaqkvea sgsaaekkgt pvedvkgdka
      721 kepsleklvk tcdeeasrip vkvtskkeea dpwkrtktes nkaekkpmlk dslqntskte
      781 vkeskipwrn kvsktpgdsa pldrsisvdp qfisrpesid pkslkqstsl eenliskmpv
      841 dkqhlirsye slngdsgtls kstssesidf wseikgpesp kvakvvnref sfvasknvep
      901 rqtvedltgk eldkkketaa kisvaaaqhg lsnipvieee sskcdaeqrv ssceatpvaa
      961 ekseaekdss tprkglrkkn lslainseay stvkkaplkr ddsnastvsa nsvsntpdts
     1021 vpvsevstpv aevplplini idtptptatp trtgsqilde edevktptne wpdpdfpkis
     1081 kwtnhsdlsn vdqvdndvtp vpskdvsvac spenspdssk kkkivrkkks sttksstkks
     1141 stkkeskdsk rtkknsskvq enlkpsepst nspkisptqr pidlikmfyt tpmalltatp
     1201 rdlskvrrak ikrkkhhsrt psissdstgs ttstattess gstcaelddd pehkrmnstr
     1261 sndsgfdgsp ristpsqssd tqrnsdssdh fpsgritppa tnlprfkkya ltdfnflkvl
     1321 gkgsfgkvll aelrgtecvy avkclkkdvv ledddvectl ierkvltlat rhpylchlfc
     1381 tfqtdshlff vmeylnggdl mfhiqksgrf setrarfyaa eiwsglnflh kkgivyrdlk
     1441 ldnvlldfeg hiriadfgmc klqifldrta dtfcgtpdym apeiikglky nqavdwwsyg
     1501 vllyemltgq spfsgcdede lfwsicnerp fiprylsqea tdilvcllek dsgkrlpghd
     1561 ialhsffqsl pwdrlerrql eapfrpaldh tldtkyfdta ftaerprltp vpeqiltsmd
     1621 qgvfrgfsyt npnatd
//
LOCUS       XP_034190339             819 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608818 [Osmia lignaria lignaria].
ACCESSION   XP_034190339
VERSION     XP_034190339.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334448.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190339.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..819
                     /product="uncharacterized protein LOC117608818"
                     /calculated_mol_wt=90998
     Region          625..815
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(658,660,664..665,668,670..671,673..674,678,681,725,
                     727,729,733..734,737..739,741..742,746,749,758,760,763,
                     767..768,771..773,775..776,780,783,792)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          658..725
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          727..758
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          760..792
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..819
                     /gene="LOC117608818"
                     /coded_by="XM_034334448.2:361..2820"
                     /db_xref="GeneID:117608818"
ORIGIN      
        1 metlstvkrl rttqnisipt iqckrefdlp hkfpskisgk snivqkghna vnttnktitq
       61 knlnnlrgke llsvnnitkl riksqdgkdl gevkvrflsq enalssktit fnkaslsyki
      121 hkpvklsivp psntgslrgs qndvetskav vksipsnkvs agsdrrsevk ekcnkaqtqy
      181 vltkiskinq qnndkmvllk endkksldkv qtlsknipky vtiqknkilf pskkknddls
      241 mknqsdshse gntknqsktn vlktqnglds eklaeskilv vkcerlsvpk skaignditr
      301 lsvntnnnes dinsgirnst venniklrrn sysikpvdnk echvstqtnp tnsidkvlne
      361 nsqneeknnt iqkqedivtl vpannkhiss enqvvpndtn nksnscqnet mrnntnsmqh
      421 edsisetska keavsvdvrk eeklsqhwdi ikealtsvkd eelrtkalqa ladcgigtpk
      481 qipikppenl ktvhdspiqt dvfglldfen filvkedtpi lerikqters tlksstldav
      541 tqtrsntkvn rhidnidlfp tlssvsmqdt ldldnyfieh fgtntnvnev kkvlstphsl
      601 ykkvgaklks dfeglqkwde ngmvnihrav indqlhelqr lllvlqaskm sidiptddge
      661 tclelaikwn asedivklll knganpvsse llhdsailla skcsspllpd lvkcvtdskl
      721 lnqvdslgfa alhycafngn legvtaliea gadvnlkdnr sgrtpffhal ennyvwiaqk
      781 llvngaianl pnfsgqtvls lidetkslsi kaalkhimg
//
LOCUS       XP_034190341             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608819 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190341
VERSION     XP_034190341.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334450.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC117608819 isoform X1"
                     /calculated_mol_wt=81098
     Region          3..>172
                     /region_name="PRK10767"
                     /note="chaperone protein DnaJ; Provisional"
                     /db_xref="CDD:236757"
     Region          606..714
                     /region_name="RanBD_RanBP3"
                     /note="Ran-binding protein 3 Ran-binding domain; cd13180"
                     /db_xref="CDD:270001"
     Site            order(620,622..624,629..634,649,651,653..654,658..660,663,
                     665,669,673,681,683,693,697)
                     /site_type="other"
                     /note="putative RAN binding site [polypeptide binding]"
                     /db_xref="CDD:270001"
     CDS             1..729
                     /gene="LOC117608819"
                     /coded_by="XM_034334450.2:199..2388"
                     /db_xref="GeneID:117608819"
ORIGIN      
        1 mvdyykilev qrtassgdik kayrklalkw hpdknpenle eankrfkeis eayevlidda
       61 krrtydqrly qkassrpgrg ftyrsyfdsp fqryfekkrr vydqygkdgl qmsggkrrhe
      121 ddfdahfagt fvfrdpaevf reffggssfe dlfsdltgtg vhrgsnrhsh pssnsistsf
      181 fgplgfggfs qfnnlfegvs gnftsfntft tfdgntggga mkrtststrf ingkkittkk
      241 vfengketim syendvlksk tvngvpqsit fdesstsrqp lsdsasdiam agqnsrtvhg
      301 dhqkasrikt hhhpplakkh dkknkeetnr psyvlkvdpp qdspgdspgd spensnseme
      361 naatennvqe sssssnkpan kiqgsvlaas kfgnpfgssd fansspnktk ssilrpsqlg
      421 avsnnqtank vvlqpakfqn pfakvtenfl eekddtvnqk ketkeakeak seekekekek
      481 ekekpteetk ptflplgast kdsenntvns stvsscdsep sfvfgqnlke rvvvaddaes
      541 sekseseepk eetanengss ellfsnasav crttarpglt lteaaqelee anrankrkys
      601 qvtlltgeeg etnvlqinck lfafdkasgg wqergrgtlr lndrdeesrl vgrtagtqrl
      661 ilntkvwpgm taeraapksl rltamdvhgd irifivqaap keiellhfll qarlkraqer
      721 qpkklaadh
//
LOCUS       XP_034190342             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608819 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190342
VERSION     XP_034190342.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334451.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC117608819 isoform X1"
                     /calculated_mol_wt=81098
     Region          3..>172
                     /region_name="PRK10767"
                     /note="chaperone protein DnaJ; Provisional"
                     /db_xref="CDD:236757"
     Region          606..714
                     /region_name="RanBD_RanBP3"
                     /note="Ran-binding protein 3 Ran-binding domain; cd13180"
                     /db_xref="CDD:270001"
     Site            order(620,622..624,629..634,649,651,653..654,658..660,663,
                     665,669,673,681,683,693,697)
                     /site_type="other"
                     /note="putative RAN binding site [polypeptide binding]"
                     /db_xref="CDD:270001"
     CDS             1..729
                     /gene="LOC117608819"
                     /coded_by="XM_034334451.2:271..2460"
                     /db_xref="GeneID:117608819"
ORIGIN      
        1 mvdyykilev qrtassgdik kayrklalkw hpdknpenle eankrfkeis eayevlidda
       61 krrtydqrly qkassrpgrg ftyrsyfdsp fqryfekkrr vydqygkdgl qmsggkrrhe
      121 ddfdahfagt fvfrdpaevf reffggssfe dlfsdltgtg vhrgsnrhsh pssnsistsf
      181 fgplgfggfs qfnnlfegvs gnftsfntft tfdgntggga mkrtststrf ingkkittkk
      241 vfengketim syendvlksk tvngvpqsit fdesstsrqp lsdsasdiam agqnsrtvhg
      301 dhqkasrikt hhhpplakkh dkknkeetnr psyvlkvdpp qdspgdspgd spensnseme
      361 naatennvqe sssssnkpan kiqgsvlaas kfgnpfgssd fansspnktk ssilrpsqlg
      421 avsnnqtank vvlqpakfqn pfakvtenfl eekddtvnqk ketkeakeak seekekekek
      481 ekekpteetk ptflplgast kdsenntvns stvsscdsep sfvfgqnlke rvvvaddaes
      541 sekseseepk eetanengss ellfsnasav crttarpglt lteaaqelee anrankrkys
      601 qvtlltgeeg etnvlqinck lfafdkasgg wqergrgtlr lndrdeesrl vgrtagtqrl
      661 ilntkvwpgm taeraapksl rltamdvhgd irifivqaap keiellhfll qarlkraqer
      721 qpkklaadh
//
LOCUS       XP_034190343             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608819 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190343
VERSION     XP_034190343.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334452.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC117608819 isoform X1"
                     /calculated_mol_wt=81098
     Region          3..>172
                     /region_name="PRK10767"
                     /note="chaperone protein DnaJ; Provisional"
                     /db_xref="CDD:236757"
     Region          606..714
                     /region_name="RanBD_RanBP3"
                     /note="Ran-binding protein 3 Ran-binding domain; cd13180"
                     /db_xref="CDD:270001"
     Site            order(620,622..624,629..634,649,651,653..654,658..660,663,
                     665,669,673,681,683,693,697)
                     /site_type="other"
                     /note="putative RAN binding site [polypeptide binding]"
                     /db_xref="CDD:270001"
     CDS             1..729
                     /gene="LOC117608819"
                     /coded_by="XM_034334452.2:220..2409"
                     /db_xref="GeneID:117608819"
ORIGIN      
        1 mvdyykilev qrtassgdik kayrklalkw hpdknpenle eankrfkeis eayevlidda
       61 krrtydqrly qkassrpgrg ftyrsyfdsp fqryfekkrr vydqygkdgl qmsggkrrhe
      121 ddfdahfagt fvfrdpaevf reffggssfe dlfsdltgtg vhrgsnrhsh pssnsistsf
      181 fgplgfggfs qfnnlfegvs gnftsfntft tfdgntggga mkrtststrf ingkkittkk
      241 vfengketim syendvlksk tvngvpqsit fdesstsrqp lsdsasdiam agqnsrtvhg
      301 dhqkasrikt hhhpplakkh dkknkeetnr psyvlkvdpp qdspgdspgd spensnseme
      361 naatennvqe sssssnkpan kiqgsvlaas kfgnpfgssd fansspnktk ssilrpsqlg
      421 avsnnqtank vvlqpakfqn pfakvtenfl eekddtvnqk ketkeakeak seekekekek
      481 ekekpteetk ptflplgast kdsenntvns stvsscdsep sfvfgqnlke rvvvaddaes
      541 sekseseepk eetanengss ellfsnasav crttarpglt lteaaqelee anrankrkys
      601 qvtlltgeeg etnvlqinck lfafdkasgg wqergrgtlr lndrdeesrl vgrtagtqrl
      661 ilntkvwpgm taeraapksl rltamdvhgd irifivqaap keiellhfll qarlkraqer
      721 qpkklaadh
//
LOCUS       XP_034190344             692 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608819 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190344
VERSION     XP_034190344.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334453.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..692
                     /product="uncharacterized protein LOC117608819 isoform X2"
                     /calculated_mol_wt=76500
     Region          3..>135
                     /region_name="PRK10767"
                     /note="chaperone protein DnaJ; Provisional"
                     /db_xref="CDD:236757"
     Region          569..677
                     /region_name="RanBD_RanBP3"
                     /note="Ran-binding protein 3 Ran-binding domain; cd13180"
                     /db_xref="CDD:270001"
     Site            order(583,585..587,592..597,612,614,616..617,621..623,626,
                     628,632,636,644,646,656,660)
                     /site_type="other"
                     /note="putative RAN binding site [polypeptide binding]"
                     /db_xref="CDD:270001"
     CDS             1..692
                     /gene="LOC117608819"
                     /coded_by="XM_034334453.2:198..2276"
                     /db_xref="GeneID:117608819"
ORIGIN      
        1 mvdyykilev qrtassgdik kayrklalkw hpdknpenle eankrfkeis eayevlidek
       61 krrvydqygk dglqmsggkr rheddfdahf agtfvfrdpa evfreffggs sfedlfsdlt
      121 gtgvhrgsnr hshpssnsis tsffgplgfg gfsqfnnlfe gvsgnftsfn tfttfdgntg
      181 ggamkrtsts trfingkkit tkkvfengke timsyendvl ksktvngvpq sitfdessts
      241 rqplsdsasd iamagqnsrt vhgdhqkasr ikthhhppla kkhdkknkee tnrpsyvlkv
      301 dppqdspgds pgdspensns emenaatenn vqesssssnk pankiqgsvl aaskfgnpfg
      361 ssdfansspn ktkssilrps qlgavsnnqt ankvvlqpak fqnpfakvte nfleekddtv
      421 nqkketkeak eakseekeke kekekekpte etkptflplg astkdsennt vnsstvsscd
      481 sepsfvfgqn lkervvvadd aesseksese epkeetanen gssellfsna savcrttarp
      541 gltlteaaqe leeanrankr kysqvtlltg eegetnvlqi ncklfafdka sggwqergrg
      601 tlrlndrdee srlvgrtagt qrlilntkvw pgmtaeraap kslrltamdv hgdirifivq
      661 aapkeiellh fllqarlkra qerqpkklaa dh
//
LOCUS       XP_034190347             694 aa            linear   INV 03-SEP-2025
DEFINITION  calcium-binding mitochondrial carrier protein Aralar1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190347
VERSION     XP_034190347.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334456.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..694
                     /product="calcium-binding mitochondrial carrier protein
                     Aralar1 isoform X1"
                     /calculated_mol_wt=77349
     Region          15..128
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          107..>173
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cl28896"
                     /db_xref="CDD:333716"
     Region          144..173
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          174..236
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(183,185,187,194,220,222,224,232)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          350..446
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          447..538
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          542..631
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..694
                     /gene="aralar1"
                     /coded_by="XM_034334456.2:809..2893"
                     /db_xref="GeneID:117608820"
ORIGIN      
        1 mvhvcdtrsn phptvtgsgy lkranterlh eifnqyasqk kngerfmtps dfvrsylglf
       61 tnpdynsdsv nllagivdts kdglisfpef qafegllcvp dalyktafql fdtngngmva
      121 fdefvevmlk telhqrmpfd messliklyf gkdkkrliny aefsqflhdf heeyateafk
      181 kfdkdcqgfi saldfqdimi sikshlltkd vrdnlvaaas tgqsgrkvsf pyfmafnsll
      241 nnmelikriy lnatnghrye evtketflhs aqmmsqitpl evdilfqlcd llhqtgsted
      301 deadsrlgki vyndlvaitp eqyfkqitkr laeikavsrp edrgvvvqil esgyrfvlgs
      361 iggavgatav ypidlvktrm qnqrtgslvg elmyrnsfdc ckkvirhegf fglyrglipq
      421 lmgvapekai kltvndfvrd kfmdkngnlp lygeilsgac aggsqviftn pleivkirlq
      481 vageiaggak vrawtvvkel glfglykgar acflrdvpfs aiyfpmyahl ktrladeggy
      541 ntplsllasg aiagvpaaal vtpadviktr lqvvarqgqt tynglldcak kifkeegpra
      601 fwkgatarvf rsspqfgvtl ftyellqrlf vvdfggsrps gsehrvpatg vaeeirstnp
      661 dhiggyqval pifngietkf glylprfqtg thek
//
LOCUS       XP_076545637             692 aa            linear   INV 03-SEP-2025
DEFINITION  calcium-binding mitochondrial carrier protein Aralar1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_076545637
VERSION     XP_076545637.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689522.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..692
                     /product="calcium-binding mitochondrial carrier protein
                     Aralar1 isoform X2"
                     /calculated_mol_wt=77123
     Region          15..126
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          105..>171
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cl28896"
                     /db_xref="CDD:333716"
     Region          142..171
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          172..234
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(181,183,185,192,218,220,222,230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          348..444
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          445..536
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          540..629
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..692
                     /gene="aralar1"
                     /coded_by="XM_076689522.1:623..2701"
                     /db_xref="GeneID:117608820"
ORIGIN      
        1 mlmdsllvqa scqegsgylk ranterlhei fnqyasqkkn gerfmtpsdf vrsylglftn
       61 pdynsdsvnl lagivdtskd glisfpefqa fegllcvpda lyktafqlfd tngngmvafd
      121 efvevmlkte lhqrmpfdme ssliklyfgk dkkrlinyae fsqflhdfhe eyateafkkf
      181 dkdcqgfisa ldfqdimisi kshlltkdvr dnlvaaastg qsgrkvsfpy fmafnsllnn
      241 melikriyln atnghryeev tketflhsaq mmsqitplev dilfqlcdll hqtgstedde
      301 adsrlgkivy ndlvaitpeq yfkqitkrla eikavsrped rgvvvqiles gyrfvlgsig
      361 gavgatavyp idlvktrmqn qrtgslvgel myrnsfdcck kvirhegffg lyrglipqlm
      421 gvapekaikl tvndfvrdkf mdkngnlply geilsgacag gsqviftnpl eivkirlqva
      481 geiaggakvr awtvvkelgl fglykgarac flrdvpfsai yfpmyahlkt rladeggynt
      541 plsllasgai agvpaaalvt padviktrlq vvarqgqtty nglldcakki fkeegprafw
      601 kgatarvfrs spqfgvtlft yellqrlfvv dfggsrpsgs ehrvpatgva eeirstnpdh
      661 iggyqvalpi fngietkfgl ylprfqtgth ek
//
LOCUS       XP_034190348             692 aa            linear   INV 03-SEP-2025
DEFINITION  calcium-binding mitochondrial carrier protein Aralar1 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034190348
VERSION     XP_034190348.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334457.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..692
                     /product="calcium-binding mitochondrial carrier protein
                     Aralar1 isoform X3"
                     /calculated_mol_wt=77122
     Region          15..128
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          107..>173
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cl28896"
                     /db_xref="CDD:333716"
     Region          144..173
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          174..236
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(183,185,187,194,220,222,224,232)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          350..446
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          447..538
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          542..629
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..692
                     /gene="aralar1"
                     /coded_by="XM_034334457.2:369..2447"
                     /db_xref="GeneID:117608820"
ORIGIN      
        1 mvhvcdtrsn phptvtgsgy lkranterlh eifnqyasqk kngerfmtps dfvrsylglf
       61 tnpdynsdsv nllagivdts kdglisfpef qafegllcvp dalyktafql fdtngngmva
      121 fdefvevmlk telhqrmpfd messliklyf gkdkkrliny aefsqflhdf heeyateafk
      181 kfdkdcqgfi saldfqdimi sikshlltkd vrdnlvaaas tgqsgrkvsf pyfmafnsll
      241 nnmelikriy lnatnghrye evtketflhs aqmmsqitpl evdilfqlcd llhqtgsted
      301 deadsrlgki vyndlvaitp eqyfkqitkr laeikavsrp edrgvvvqil esgyrfvlgs
      361 iggavgatav ypidlvktrm qnqrtgslvg elmyrnsfdc ckkvirhegf fglyrglipq
      421 lmgvapekai kltvndfvrd kfmdkngnlp lygeilsgac aggsqviftn pleivkirlq
      481 vageiaggak vrawtvvkel glfglykgar acflrdvpfs aiyfpmyahl ktrladeggy
      541 ntplsllasg aiagvpaaal vtpadviktr lqvvarqgqt tynglldcak kifkeegpra
      601 fwkgatvfrs spqfgvtlft yellqrlfvv dfggsrpsgs ehrvpatgva eeirstnpdh
      661 iggyqvalpi fngietkfgl ylprfqtgth ek
//
LOCUS       XP_076545638             689 aa            linear   INV 03-SEP-2025
DEFINITION  calcium-binding mitochondrial carrier protein Aralar1 isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_076545638
VERSION     XP_076545638.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689523.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..689
                     /product="calcium-binding mitochondrial carrier protein
                     Aralar1 isoform X4"
                     /calculated_mol_wt=76876
     Region          12..123
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          102..>168
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cl28896"
                     /db_xref="CDD:333716"
     Region          139..168
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          169..231
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(178,180,182,189,215,217,219,227)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          345..441
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          442..533
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          537..626
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..689
                     /gene="aralar1"
                     /coded_by="XM_076689523.1:188..2257"
                     /db_xref="GeneID:117608820"
ORIGIN      
        1 mheksidtce egsgylkran terlheifnq yasqkknger fmtpsdfvrs ylglftnpdy
       61 nsdsvnllag ivdtskdgli sfpefqafeg llcvpdalyk tafqlfdtng ngmvafdefv
      121 evmlktelhq rmpfdmessl iklyfgkdkk rlinyaefsq flhdfheeya teafkkfdkd
      181 cqgfisaldf qdimisiksh lltkdvrdnl vaaastgqsg rkvsfpyfma fnsllnnmel
      241 ikriylnatn ghryeevtke tflhsaqmms qitplevdil fqlcdllhqt gsteddeads
      301 rlgkivyndl vaitpeqyfk qitkrlaeik avsrpedrgv vvqilesgyr fvlgsiggav
      361 gatavypidl vktrmqnqrt gslvgelmyr nsfdcckkvi rhegffglyr glipqlmgva
      421 pekaikltvn dfvrdkfmdk ngnlplygei lsgacaggsq viftnpleiv kirlqvagei
      481 aggakvrawt vvkelglfgl ykgaracflr dvpfsaiyfp myahlktrla deggyntpls
      541 llasgaiagv paaalvtpad viktrlqvva rqgqttyngl ldcakkifke egprafwkga
      601 tarvfrsspq fgvtlftyel lqrlfvvdfg gsrpsgsehr vpatgvaeei rstnpdhigg
      661 yqvalpifng ietkfglylp rfqtgthek
//
LOCUS       XP_034190352             682 aa            linear   INV 03-SEP-2025
DEFINITION  calcium-binding mitochondrial carrier protein Aralar1 isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034190352
VERSION     XP_034190352.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334461.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..682
                     /product="calcium-binding mitochondrial carrier protein
                     Aralar1 isoform X5"
                     /calculated_mol_wt=76073
     Region          15..128
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          107..>173
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cl28896"
                     /db_xref="CDD:333716"
     Region          144..173
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          174..236
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(183,185,187,194,220,222,224,232)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          338..434
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          435..526
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          530..619
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..682
                     /gene="aralar1"
                     /coded_by="XM_034334461.2:367..2415"
                     /db_xref="GeneID:117608820"
ORIGIN      
        1 mvhvcdtrsn phptvtgsgy lkranterlh eifnqyasqk kngerfmtps dfvrsylglf
       61 tnpdynsdsv nllagivdts kdglisfpef qafegllcvp dalyktafql fdtngngmva
      121 fdefvevmlk telhqrmpfd messliklyf gkdkkrliny aefsqflhdf heeyateafk
      181 kfdkdcqgfi saldfqdimi sikshlltkd vrdnlvaaas tgqsgrkvsf pyfmafnsll
      241 nnmelikriy lnatnghrye evtketflhs aqmmsqitpl evdilfqlcd llhqtgkivy
      301 ndlvaitpeq yfkqitkrla eikavsrped rgvvvqiles gyrfvlgsig gavgatavyp
      361 idlvktrmqn qrtgslvgel myrnsfdcck kvirhegffg lyrglipqlm gvapekaikl
      421 tvndfvrdkf mdkngnlply geilsgacag gsqviftnpl eivkirlqva geiaggakvr
      481 awtvvkelgl fglykgarac flrdvpfsai yfpmyahlkt rladeggynt plsllasgai
      541 agvpaaalvt padviktrlq vvarqgqtty nglldcakki fkeegprafw kgatarvfrs
      601 spqfgvtlft yellqrlfvv dfggsrpsgs ehrvpatgva eeirstnpdh iggyqvalpi
      661 fngietkfgl ylprfqtgth ek
//
LOCUS       XP_076545639             679 aa            linear   INV 03-SEP-2025
DEFINITION  calcium-binding mitochondrial carrier protein Aralar1 isoform X6
            [Osmia lignaria lignaria].
ACCESSION   XP_076545639
VERSION     XP_076545639.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689524.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..679
                     /product="calcium-binding mitochondrial carrier protein
                     Aralar1 isoform X6"
                     /calculated_mol_wt=75704
     Region          2..113
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          92..>158
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cl28896"
                     /db_xref="CDD:333716"
     Region          129..158
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          159..221
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(168,170,172,179,205,207,209,217)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          335..431
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          432..523
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          527..616
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..679
                     /gene="aralar1"
                     /coded_by="XM_076689524.1:292..2331"
                     /db_xref="GeneID:117608820"
ORIGIN      
        1 mgsgylkran terlheifnq yasqkknger fmtpsdfvrs ylglftnpdy nsdsvnllag
       61 ivdtskdgli sfpefqafeg llcvpdalyk tafqlfdtng ngmvafdefv evmlktelhq
      121 rmpfdmessl iklyfgkdkk rlinyaefsq flhdfheeya teafkkfdkd cqgfisaldf
      181 qdimisiksh lltkdvrdnl vaaastgqsg rkvsfpyfma fnsllnnmel ikriylnatn
      241 ghryeevtke tflhsaqmms qitplevdil fqlcdllhqt gsteddeads rlgkivyndl
      301 vaitpeqyfk qitkrlaeik avsrpedrgv vvqilesgyr fvlgsiggav gatavypidl
      361 vktrmqnqrt gslvgelmyr nsfdcckkvi rhegffglyr glipqlmgva pekaikltvn
      421 dfvrdkfmdk ngnlplygei lsgacaggsq viftnpleiv kirlqvagei aggakvrawt
      481 vvkelglfgl ykgaracflr dvpfsaiyfp myahlktrla deggyntpls llasgaiagv
      541 paaalvtpad viktrlqvva rqgqttyngl ldcakkifke egprafwkga tarvfrsspq
      601 fgvtlftyel lqrlfvvdfg gsrpsgsehr vpatgvaeei rstnpdhigg yqvalpifng
      661 ietkfglylp rfqtgthek
//
LOCUS       XP_076545640             648 aa            linear   INV 03-SEP-2025
DEFINITION  calcium-binding mitochondrial carrier protein Aralar1 isoform X7
            [Osmia lignaria lignaria].
ACCESSION   XP_076545640
VERSION     XP_076545640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689525.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..648
                     /product="calcium-binding mitochondrial carrier protein
                     Aralar1 isoform X7"
                     /calculated_mol_wt=72048
     Region          <1..82
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          61..>127
                     /region_name="EFh_MICU"
                     /note="EF-hand, calcium binding motif, found in
                     mitochondrial calcium uptake proteins MICU1, MICU2, MICU3,
                     and similar proteins; cl28896"
                     /db_xref="CDD:333716"
     Region          98..127
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320080"
     Region          128..190
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Site            order(137,139,141,148,174,176,178,186)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          304..400
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          401..492
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          496..585
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..648
                     /gene="aralar1"
                     /coded_by="XM_076689525.1:759..2705"
                     /db_xref="GeneID:117608820"
ORIGIN      
        1 mtpsdfvrsy lglftnpdyn sdsvnllagi vdtskdglis fpefqafegl lcvpdalykt
       61 afqlfdtngn gmvafdefve vmlktelhqr mpfdmessli klyfgkdkkr linyaefsqf
      121 lhdfheeyat eafkkfdkdc qgfisaldfq dimisikshl ltkdvrdnlv aaastgqsgr
      181 kvsfpyfmaf nsllnnmeli kriylnatng hryeevtket flhsaqmmsq itplevdilf
      241 qlcdllhqtg steddeadsr lgkivyndlv aitpeqyfkq itkrlaeika vsrpedrgvv
      301 vqilesgyrf vlgsiggavg atavypidlv ktrmqnqrtg slvgelmyrn sfdcckkvir
      361 hegffglyrg lipqlmgvap ekaikltvnd fvrdkfmdkn gnlplygeil sgacaggsqv
      421 iftnpleivk irlqvageia ggakvrawtv vkelglfgly kgaracflrd vpfsaiyfpm
      481 yahlktrlad eggyntplsl lasgaiagvp aaalvtpadv iktrlqvvar qgqttyngll
      541 dcakkifkee gprafwkgat arvfrsspqf gvtlftyell qrlfvvdfgg srpsgsehrv
      601 patgvaeeir stnpdhiggy qvalpifngi etkfglylpr fqtgthek
//
LOCUS       XP_034190354             634 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate receptor interacting protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190354
VERSION     XP_034190354.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..634
                     /product="glutamate receptor interacting protein isoform
                     X1"
                     /calculated_mol_wt=67936
     Region          65..149
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(77..83,93,96,126,130,133..134)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          162..244
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(175..181,190,193,225,228..229)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          274..346
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(286..292,303,306,336,340,343..344)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          372..447
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(381..387,393,396,426,430,433..434)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          541..623
                     /region_name="PDZ7_GRIP1-2-like"
                     /note="PDZ domain 7 of glutamate receptor-interacting
                     protein 1 (GRIP1) and GRIP2, and related domains; cd06685"
                     /db_xref="CDD:467173"
     Site            order(555..561,572,575,603,607,610..611)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467173"
     Site            order(593,596,601,604..605,608..609)
                     /site_type="other"
                     /note="GRASP-1 binding site [polypeptide binding]"
                     /db_xref="CDD:467173"
     CDS             1..634
                     /gene="Grip"
                     /coded_by="XM_034334463.2:1..1905"
                     /db_xref="GeneID:117608821"
ORIGIN      
        1 mfsslrlttm rshknrarpg svatsassds araddittqs yhphsfllld dqdspvstps
       61 svsskvaqvr vereggslgv tlrggvsral vvtgvkadgp aakegrvrpg drllavddte
      121 lrgltlaeaq ralrrssdap vasltieydv anmeearaat sgpllvqler glsgelgltv
      181 retatgvyie slrpastadr cgalqpgdkl lavdetpvqd avtaakllrn nfdnsriarl
      241 qilprppsts qrtvkrraqp qaqqsssqtl qtkesltivl rpdhkgfgla lkpaedrlny
      301 vvslleaggp aersgvilpg dkvlavnrrm lrdlqpaevt siletsqvie lvteykvggp
      361 vvpssgvftv rvarpvggqp dvgltvnedl iitevrkgsl ayrtgslapr drllaidgqk
      421 ldpgdlrqaa qllhrpgssv valtvrkpdp tsetreycrd ssigsdtvqq yppgilrpar
      481 eslpsvdsav dswgelgdns gtgpeilrlw dtasvdsgql dlpmppfpgp qecsnsdrpq
      541 qivhvslhkd pvyedfgfsv sdglyergvy inrlrpggpc dgilrpydri lrvndasted
      601 cdcclavpli aaagprldlt varpmsppni iktl
//
LOCUS       XP_034190355             630 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate receptor interacting protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190355
VERSION     XP_034190355.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334464.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="glutamate receptor interacting protein isoform
                     X2"
                     /calculated_mol_wt=67384
     Region          65..149
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(77..83,93,96,126,130,133..134)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          162..244
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(175..181,190,193,225,228..229)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          274..346
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(286..292,303,306,336,340,343..344)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          372..447
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(381..387,393,396,426,430,433..434)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          537..619
                     /region_name="PDZ7_GRIP1-2-like"
                     /note="PDZ domain 7 of glutamate receptor-interacting
                     protein 1 (GRIP1) and GRIP2, and related domains; cd06685"
                     /db_xref="CDD:467173"
     Site            order(551..557,568,571,599,603,606..607)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467173"
     Site            order(589,592,597,600..601,604..605)
                     /site_type="other"
                     /note="GRASP-1 binding site [polypeptide binding]"
                     /db_xref="CDD:467173"
     CDS             1..630
                     /gene="Grip"
                     /coded_by="XM_034334464.2:1..1893"
                     /db_xref="GeneID:117608821"
ORIGIN      
        1 mfsslrlttm rshknrarpg svatsassds araddittqs yhphsfllld dqdspvstps
       61 svsskvaqvr vereggslgv tlrggvsral vvtgvkadgp aakegrvrpg drllavddte
      121 lrgltlaeaq ralrrssdap vasltieydv anmeearaat sgpllvqler glsgelgltv
      181 retatgvyie slrpastadr cgalqpgdkl lavdetpvqd avtaakllrn nfdnsriarl
      241 qilprppsts qrtvkrraqp qaqqsssqtl qtkesltivl rpdhkgfgla lkpaedrlny
      301 vvslleaggp aersgvilpg dkvlavnrrm lrdlqpaevt siletsqvie lvteykvggp
      361 vvpssgvftv rvarpvggqp dvgltvnedl iitevrkgsl ayrtgslapr drllaidgqk
      421 ldpgdlrqaa qllhrpgssv valtvrkpdp tsetrdssig sdtvqqyppg ilrpareslp
      481 svdsavdswg elgdnsgtgp eilrlwdtas vdsgqldlpm ppfpgpqecs nsdrpqqivh
      541 vslhkdpvye dfgfsvsdgl yergvyinrl rpggpcdgil rpydrilrvn dastedcdcc
      601 lavpliaaag prldltvarp msppniiktl
//
LOCUS       XP_034190638             438 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-binding protein Ets97D isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190638
VERSION     XP_034190638.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334747.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..438
                     /product="DNA-binding protein Ets97D isoform X1"
                     /calculated_mol_wt=50305
     Region          77..154
                     /region_name="GABP-alpha"
                     /note="GA-binding protein alpha chain; pfam11620"
                     /db_xref="CDD:463311"
     Region          180..267
                     /region_name="SAM_PNT-GABP-alpha"
                     /note="Sterile alpha motif (SAM)/Pointed domain of
                     GA-binding protein (GABP) alpha chain; cd08534"
                     /db_xref="CDD:176084"
     Region          332..416
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..438
                     /gene="Ets97D"
                     /coded_by="XM_034334747.2:307..1623"
                     /db_xref="GeneID:117608955"
ORIGIN      
        1 mdlgngrknc eksfdtddds dyaiikrikm epeailsqed dimaqlqqes sdlevepsfa
       61 legavngddc negvlmqhmd ireplstlrn lleqrlgvel tdysfwlqda qmleshknlv
      121 dqcvqgeglv qvnvqikanq rrinivdvlk paedyielae nstsvpined skrniirwmv
      181 dpqykkeqer lkipadpkew tqthvkhwlq wavrqfnlas vkladwnitg qqlcnltlee
      241 fqakvpldpg evfwthlell rkckfvavvq kdppaspten nveksikvrn qktpksrpvv
      301 nqqarvvsvp feniestpit iatssrsvns gqiqlwqfll elltdrehrg aiqwvgtege
      361 fklnqpeava qlwgarknkp smnyeklsra lryyydgdmi skvhgkrfvy kfvcdlkqvl
      421 gysaaelsrl veegrryf
//
LOCUS       XP_034190639             409 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-binding protein Ets97D isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190639
VERSION     XP_034190639.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334748.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="DNA-binding protein Ets97D isoform X2"
                     /calculated_mol_wt=46975
     Region          48..125
                     /region_name="GABP-alpha"
                     /note="GA-binding protein alpha chain; pfam11620"
                     /db_xref="CDD:463311"
     Region          151..238
                     /region_name="SAM_PNT-GABP-alpha"
                     /note="Sterile alpha motif (SAM)/Pointed domain of
                     GA-binding protein (GABP) alpha chain; cd08534"
                     /db_xref="CDD:176084"
     Region          303..387
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..409
                     /gene="Ets97D"
                     /coded_by="XM_034334748.2:523..1752"
                     /db_xref="GeneID:117608955"
ORIGIN      
        1 mepeailsqe ddimaqlqqe ssdlevepsf alegavngdd cnegvlmqhm direplstlr
       61 nlleqrlgve ltdysfwlqd aqmleshknl vdqcvqgegl vqvnvqikan qrrinivdvl
      121 kpaedyiela enstsvpine dskrniirwm vdpqykkeqe rlkipadpke wtqthvkhwl
      181 qwavrqfnla svkladwnit gqqlcnltle efqakvpldp gevfwthlel lrkckfvavv
      241 qkdppaspte nnveksikvr nqktpksrpv vnqqarvvsv pfeniestpi tiatssrsvn
      301 sgqiqlwqfl lelltdrehr gaiqwvgteg efklnqpeav aqlwgarknk psmnyeklsr
      361 alryyydgdm iskvhgkrfv ykfvcdlkqv lgysaaelsr lveegrryf
//
LOCUS       XP_034190640             409 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-binding protein Ets97D isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190640
VERSION     XP_034190640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334749.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="DNA-binding protein Ets97D isoform X2"
                     /calculated_mol_wt=46975
     Region          48..125
                     /region_name="GABP-alpha"
                     /note="GA-binding protein alpha chain; pfam11620"
                     /db_xref="CDD:463311"
     Region          151..238
                     /region_name="SAM_PNT-GABP-alpha"
                     /note="Sterile alpha motif (SAM)/Pointed domain of
                     GA-binding protein (GABP) alpha chain; cd08534"
                     /db_xref="CDD:176084"
     Region          303..387
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..409
                     /gene="Ets97D"
                     /coded_by="XM_034334749.2:435..1664"
                     /db_xref="GeneID:117608955"
ORIGIN      
        1 mepeailsqe ddimaqlqqe ssdlevepsf alegavngdd cnegvlmqhm direplstlr
       61 nlleqrlgve ltdysfwlqd aqmleshknl vdqcvqgegl vqvnvqikan qrrinivdvl
      121 kpaedyiela enstsvpine dskrniirwm vdpqykkeqe rlkipadpke wtqthvkhwl
      181 qwavrqfnla svkladwnit gqqlcnltle efqakvpldp gevfwthlel lrkckfvavv
      241 qkdppaspte nnveksikvr nqktpksrpv vnqqarvvsv pfeniestpi tiatssrsvn
      301 sgqiqlwqfl lelltdrehr gaiqwvgteg efklnqpeav aqlwgarknk psmnyeklsr
      361 alryyydgdm iskvhgkrfv ykfvcdlkqv lgysaaelsr lveegrryf
//
LOCUS       XP_076545641             350 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 35 member E1 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_076545641
VERSION     XP_076545641.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689526.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="solute carrier family 35 member E1 homolog"
                     /calculated_mol_wt=39434
     Region          12..303
                     /region_name="TPT"
                     /note="Triose-phosphate Transporter family; pfam03151"
                     /db_xref="CDD:308657"
     CDS             1..350
                     /gene="LOC117608824"
                     /coded_by="XM_076689526.1:359..1411"
                     /db_xref="GeneID:117608824"
ORIGIN      
        1 mddrrnsrev atilflcllw ygissssnvv gkmllsefpy pmtvtmvqlt sitiysgpff
       61 nlwgvrkyas nipwgyylrl ivplalgkfl anvfshvsiw kvpvsyahtv katmpfftvl
      121 lsriilrekq twkvylslvp ivvgvavatl telsfnmigl lsalastmaf slqniyskkv
      181 lrdtgihhlr llhilgrlal ilflpiwlmy dlrrliydpt thgsaylssn iigllfldgv
      241 lnwfqniiaf svlsivtplt yavasaskri fviavtllvl gnpvtwlnvf gmtlaiigvl
      301 yynkakydqr lekesetalp kyydknrngd ssssfmingw tgkkqqlyav
//
LOCUS       XP_034190336             336 aa            linear   INV 03-SEP-2025
DEFINITION  venom allergen 5 [Osmia lignaria lignaria].
ACCESSION   XP_034190336
VERSION     XP_034190336.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334445.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190336.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="venom allergen 5"
                     /calculated_mol_wt=38036
     Region          143..287
                     /region_name="CAP_euk"
                     /note="Eukaryotic CAP (cysteine-rich secretory proteins,
                     antigen 5, and pathogenesis-related 1 proteins) domain
                     proteins; cd05380"
                     /db_xref="CDD:349399"
     CDS             1..336
                     /gene="LOC117608816"
                     /coded_by="XM_034334445.2:16..1026"
                     /db_xref="GeneID:117608816"
ORIGIN      
        1 mqlvpsietg spnnytvqpm gqvnikaarr awrllvsvcv rrpvltlesl vnrlvssadr
       61 patcvrvnli lihprgyrts svvqgtkdcd frreetlkmk rqrdgiwlil vlvlspmvlv
      121 dsswpscvgk tmlrssglsc eekqaildeh nrlrqlvalg qihgqpsaan mremvwddel
      181 aaiaqrwanr cteihdnsrn vrrfavgqni awtwttrppg pfddepnwrq ritdwfnevq
      241 hyragysdat ghytqivwgd tylvgcgysf yydpargytk yyvcnygpsg nvlgfqpyqs
      301 gqpacgsygm vysnryaglc sgqnyyhlga lcpygr
//
LOCUS       XP_076545642             204 aa            linear   INV 03-SEP-2025
DEFINITION  protein kinase C-like 1 [Osmia lignaria lignaria].
ACCESSION   XP_076545642
VERSION     XP_076545642.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689527.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..204
                     /product="protein kinase C-like 1"
                     /calculated_mol_wt=22596
     Region          101..161
                     /region_name="C1_nPKC_theta-like_rpt1"
                     /note="first protein kinase C conserved region 1 (C1
                     domain) found in novel protein kinase C (nPKC) theta,
                     delta, and similar proteins; cd20834"
                     /db_xref="CDD:410384"
     Site            order(113..119,127..131,134)
                     /site_type="other"
                     /note="putative DAG/PE binding site [chemical binding]"
                     /db_xref="CDD:410384"
     CDS             1..204
                     /gene="LOC143305511"
                     /coded_by="XM_076689527.1:8..622"
                     /db_xref="GeneID:143305511"
ORIGIN      
        1 mmftggshar rrnagggsgs srrssvnhks pqarvgaegy tyrtripvln padlppstst
       61 gkyvyktrvp rrdiasastl phennvggai trrrgavkhn kvhivrghrl vakffrtptf
      121 cafckdflwg fgkqgyqcqa cqtavhkkch dklltkcpes gresentivs vricnlqevl
      181 rvssalrqkr nvpfvneqnf sddp
//
LOCUS       XP_034190357              89 aa            linear   INV 03-SEP-2025
DEFINITION  acyl-CoA binding protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190357
VERSION     XP_034190357.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334466.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="acyl-CoA binding protein 1"
                     /calculated_mol_wt=9882
     Region          3..87
                     /region_name="ACBP"
                     /note="Acyl CoA binding protein (ACBP) binds thiol esters
                     of long fatty acids and coenzyme A in a one-to-one binding
                     mode with high specificity and affinity. Acyl-CoAs are
                     important intermediates in fatty lipid synthesis and fatty
                     acid degradation and play a...; cl00221"
                     /db_xref="CDD:469667"
     Site            order(11,14..15,17,20..21,23,26..27,29..30,33..34,51..52,
                     55..56,75)
                     /site_type="other"
                     /note="acyl-CoA binding pocket [chemical binding]"
                     /db_xref="CDD:238248"
     Site            order(15,30,33..34,56,75)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:238248"
     CDS             1..89
                     /gene="Acbp1"
                     /coded_by="XM_034334466.2:423..692"
                     /db_xref="GeneID:117608823"
ORIGIN      
        1 mslveqfeka aeevkelssq psdadllely slfkqatvgd cntarpgmld fkgkakwdaw
       61 drikgmnqds akekyvqkvk qlvssigkk
//
LOCUS       XP_034190278             909 aa            linear   INV 03-SEP-2025
DEFINITION  nonsense-mediated mRNA decay factor SMG8 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190278
VERSION     XP_034190278.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334387.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..909
                     /product="nonsense-mediated mRNA decay factor SMG8 isoform
                     X1"
                     /calculated_mol_wt=101890
     Region          19..898
                     /region_name="Smg8_Smg9"
                     /note="pfam10220"
                     /db_xref="CDD:463005"
     CDS             1..909
                     /gene="LOC117608787"
                     /coded_by="XM_034334387.2:99..2828"
                     /db_xref="GeneID:117608787"
ORIGIN      
        1 mrpqkfripc diesfvptdk kvvivsvfgk sqyrakgckt nmlssilqvg lldepevnkd
       61 sfceiegyyd tkdrtiylhl rglldthtlv teydrfiekq dckdflsvwa hmrdkyaral
      121 lvlfhishvi vlthpthtfd ysyvhlfrai divrqkvspq lsdvlsciys lpkdwvsnvr
      181 pcsprvlfyf etcpsvfqdp tsganikkle hsledqiyqv lrknrivtni ssnslfaipa
      241 nqefvyvhtg tnesrdilgy marklmqsck vssggstsrs lasqdsnvqm ddtetetrsf
      301 rsflqqhinl afargfddnv grhstpayfe ipsvqifcev ankvydyfih gtdkelltlh
      361 slldtdvkfs ksrcskvlpr alqtyqenlp qhytrayhet klahamavfe mhargplfee
      421 fseklqaece khwrsgrqmc evlsltgnpc tnpihrggse gagggeqadq rdsdndlpir
      481 ehcsgvryic acncgrcqgs redpfslrqa nydyfqilak qcgcaqlesi qfpvfqpsth
      541 nyrpaqlfst krkdsfghge splpqgntqa cslgvntlnd dirtpygsgg qspptsetne
      601 dvpklsskts ltpsdahkvv ievsdsdadn ardvtekslv rqpstteylp gmlhtespag
      661 llpqfsswsl vclgpsslys hnlglqhqag ilptsafllp wdvtvrlehq kergslwpti
      721 gdhgthgycp rgknfqnmnt irgrkskggk efvvkifvgv eyecprghrf masapdkvlk
      781 atgngivkds gnkvtsstad mplyfpcpcr qtkpliaqlm rihvvtpkap vhvtlnprvq
      841 pgpppcpifv tgcdepikls qsaywvlrlp yvymdekgpy lapkeavpts hgrllagmyg
      901 isevvpekk
//
LOCUS       XP_034190279             906 aa            linear   INV 03-SEP-2025
DEFINITION  nonsense-mediated mRNA decay factor SMG8 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190279
VERSION     XP_034190279.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334388.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..906
                     /product="nonsense-mediated mRNA decay factor SMG8 isoform
                     X2"
                     /calculated_mol_wt=101575
     Region          19..895
                     /region_name="Smg8_Smg9"
                     /note="pfam10220"
                     /db_xref="CDD:463005"
     CDS             1..906
                     /gene="LOC117608787"
                     /coded_by="XM_034334388.2:99..2819"
                     /db_xref="GeneID:117608787"
ORIGIN      
        1 mrpqkfripc diesfvptdk kvvivsvfgk sqyrakgckt nmlssilqvg lldepevnkd
       61 sfceiegyyd tkdrtiylhl rglldthtlv teydrfiekq dckdflsvwa hmrdkyaral
      121 lvlfhishvi vlthpthtfd ysyvhlfrai divrqkvspq lsdvlsciys lpkdwvsnvr
      181 pcsprvlfyf etcpsvfqdp tsganikkle hsledqiyqv lrknrivtni ssnslfaipa
      241 nqefvyvhtg tnesrdilgy marklmqsck vssggstsrs lasqdsnvqm ddtetetrsf
      301 rsflqqhinl afargfddnv grhstpayfe ipsvqifcev ankvydyfih gtdkelltlh
      361 slldtdvkfs ksrcskvlpr alqtyqenlp qhytrayhet klahamavfe mhargplfee
      421 fseklqaece khwrsgrqmc evlsltgnpc tnpihrggse gagggeqadq rdsdndlpir
      481 ehcsgvryic acncgrcqgs redpfslrqa nydyfqilak qcgcaqlesi qfpvfqpsth
      541 nyrpaqlfst krkdsfghge splpqgntqa cslgvntlnd dirtpygsgg qspptsetne
      601 dvpklsskts ltpsdahkvv ievsdsdadn arekslvrqp stteylpgml htespagllp
      661 qfsswslvcl gpsslyshnl glqhqagilp tsafllpwdv tvrlehqker gslwptigdh
      721 gthgycprgk nfqnmntirg rkskggkefv vkifvgveye cprghrfmas apdkvlkatg
      781 ngivkdsgnk vtsstadmpl yfpcpcrqtk pliaqlmrih vvtpkapvhv tlnprvqpgp
      841 ppcpifvtgc depiklsqsa ywvlrlpyvy mdekgpylap keavptshgr llagmygise
      901 vvpekk
//
LOCUS       XP_034190285             658 aa            linear   INV 03-SEP-2025
DEFINITION  protein brambleberry [Osmia lignaria lignaria].
ACCESSION   XP_034190285
VERSION     XP_034190285.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334394.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..658
                     /product="protein brambleberry"
                     /calculated_mol_wt=74904
     Region          <161..>332
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..658
                     /gene="LOC117608788"
                     /coded_by="XM_034334394.2:1..1977"
                     /db_xref="GeneID:117608788"
ORIGIN      
        1 mqsvclftll vvtlscrdgr asvlewfwrr dtddttvlva dgvplisipy esmtedekfl
       61 qeaskfteiq tsspletcqh kvimkiktsc tgmteeelak lsvnllncqa avegrkmfpc
      121 teemslkqct tdmdpdmwna yhlmsnrara vcyaarstqf ralteltvnk lmqtahtqik
      181 tlsslkegqd rleeqtveal aslskgnval leqqkhlkda qtsahnivat nlrelsnera
      241 lirsghaqla amtedirkkl eeanrnleqq amergenhqe vledliniqd qaqliwdkie
      301 sstnrifaqh eeallqyert lqklaqinet iqyvwnvtnv mrtevdqkls wltsyigdtg
      361 eqmqrmyrig lhivylllam vvaafvhapl ltritilglv plnlvtylkh gmeacldfts
      421 mtvlillitt mhfvmigiqs lfglksrnvr aepvqivnqn ghlngtsrsy vsssynnlqp
      481 prnpsisfyv klkritrefy nlvryqinnc iqkcsalmqs vaswsrqslt qreelscsym
      541 psrrnredli dsyenefpsm sedvtdfess rlmeernesl ddldnltdah dlklrlrrre
      601 arssyarsps rsatpsscas srvlcnaltk sgkkcrliai nghiycrrhs ngssimgd
//
LOCUS       XP_034190463             316 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608864 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190463
VERSION     XP_034190463.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334572.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="uncharacterized protein LOC117608864 isoform X1"
                     /calculated_mol_wt=35830
     CDS             1..316
                     /gene="LOC117608864"
                     /coded_by="XM_034334572.2:825..1775"
                     /db_xref="GeneID:117608864"
ORIGIN      
        1 mslvdkpvse eckrritlpl dsffdgnveq iidvmrkeae skevieckea yrlivwtila
       61 gagtqppslv nrrqltkwed dmdgyvpqkd apehwevplp trasilsnrv sqvaariknn
      121 swhilgmlga narnrawgfa vawalldggi edemyvrwsm qvksglcysg eefaqgiisi
      181 lilwayqrsg ksdiysrvgy gnsfstqsed crsaesflfs ltltnfpvnl rqsrvcpfcl
      241 fselrlcvlt dlrgskegka ernykdvlkm iprgcewmkt aaeahnremh slngnidslv
      301 dawaeciiml fntfty
//
LOCUS       XP_034190464              66 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608864 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190464
VERSION     XP_034190464.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334573.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..66
                     /product="uncharacterized protein LOC117608864 isoform X2"
                     /calculated_mol_wt=7714
     CDS             1..66
                     /gene="LOC117608864"
                     /coded_by="XM_034334573.2:648..848"
                     /db_xref="GeneID:117608864"
ORIGIN      
        1 mfpavsgpat dhitdmeide qqkknqqeek ekekdeeeet ekkedkreek lstsqkrhpq
       61 ssgffy
//
LOCUS       XP_034190465              56 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608864 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190465
VERSION     XP_034190465.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..56
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..56
                     /product="uncharacterized protein LOC117608864 isoform X3"
                     /calculated_mol_wt=6764
     CDS             1..56
                     /gene="LOC117608864"
                     /coded_by="XM_034334574.2:210..380"
                     /db_xref="GeneID:117608864"
ORIGIN      
        1 msedkmeide qqkknqqeek ekekdeeeet ekkedkreek lstsqkrhpq ssgffy
//
LOCUS       XP_034190458             342 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin Y [Osmia lignaria lignaria].
ACCESSION   XP_034190458
VERSION     XP_034190458.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334567.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..342
                     /product="cyclin Y"
                     /calculated_mol_wt=38785
     Region          164..260
                     /region_name="CYCLIN_CCNY_like"
                     /note="cyclin box found in cyclin-Y (CCNY) family;
                     cd20540"
                     /db_xref="CDD:410243"
     CDS             1..342
                     /gene="CycY"
                     /coded_by="XM_034334567.2:580..1608"
                     /db_xref="GeneID:117608862"
ORIGIN      
        1 mgnkssccvn sspqvgrkel esegitrgle ehlpeggisv nnlqhisere pedwdsdpsl
       61 hpcagtifme rskqaiengm vrkksqhqia dvrslkksss cstiylddst vsqpnlkntv
      121 kcvalavyyh iknrtsqrqi difdeklhpl tregvtedyd kqnpehkqiy kfvktlfnaa
      181 qltaecaiit lvylerllty aeiditpanw krivlgaill askvwddqav wnvdycqilk
      241 ditvedmnel erqflemlqf ninvpssvya kyyfdlrtla eaneltfpse plskekaqkl
      301 eamsrvyedk vtaealrtgf kkwssldnic iggprrsiai ls
//
LOCUS       XP_076545643             239 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608869 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545643
VERSION     XP_076545643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689528.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="uncharacterized protein LOC117608869 isoform X1"
                     /calculated_mol_wt=27432
     Region          9..89
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..239
                     /gene="LOC117608869"
                     /coded_by="XM_076689528.1:651..1370"
                     /db_xref="GeneID:117608869"
ORIGIN      
        1 mnarggyrcs fkgcpsasse ergiketlfr fpkdverskl wilacnreel lvktatqlys
       61 gyrvcklhfa tnmflnyert rlqphavpss ivrnndeess skslnveinc innlniqeen
      121 iaidifnesn qdnthidkti pnvsdvsevg tqtslslsst spqkahlyqq iraykrkian
      181 lesqlheakk rkedinslda lldkyfpqqt skylktqvrl fqkdakgrqy elhlysytv
//
LOCUS       XP_034190484             239 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608869 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190484
VERSION     XP_034190484.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334593.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="uncharacterized protein LOC117608869 isoform X2"
                     /calculated_mol_wt=27360
     Region          9..89
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..239
                     /gene="LOC117608869"
                     /coded_by="XM_034334593.2:653..1372"
                     /db_xref="GeneID:117608869"
ORIGIN      
        1 mnarggyrcs fkgcpsasse ergiketlfr fpkdverskl wilacnreel lvktatqlys
       61 gyrvcklhfa tnmflnyert rlqphavpss ivrnndeess skslnveinc innlniqeen
      121 iaidifnesn qdnthidkti pnvsdvsevg tqtslslsst spqkahlyqq iraykrkian
      181 lesqlheakk rkedinslda lldkyfpqqt skylktqvrl fqkdakaraq ptiwrsfsh
//
LOCUS       XP_034190485             239 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608869 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190485
VERSION     XP_034190485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="uncharacterized protein LOC117608869 isoform X1"
                     /calculated_mol_wt=27432
     Region          9..89
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..239
                     /gene="LOC117608869"
                     /coded_by="XM_034334594.2:544..1263"
                     /db_xref="GeneID:117608869"
ORIGIN      
        1 mnarggyrcs fkgcpsasse ergiketlfr fpkdverskl wilacnreel lvktatqlys
       61 gyrvcklhfa tnmflnyert rlqphavpss ivrnndeess skslnveinc innlniqeen
      121 iaidifnesn qdnthidkti pnvsdvsevg tqtslslsst spqkahlyqq iraykrkian
      181 lesqlheakk rkedinslda lldkyfpqqt skylktqvrl fqkdakgrqy elhlysytv
//
LOCUS       XP_034190486             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608869 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190486
VERSION     XP_034190486.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334595.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117608869 isoform X3"
                     /calculated_mol_wt=26034
     Region          9..89
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..228
                     /gene="LOC117608869"
                     /coded_by="XM_034334595.2:650..1336"
                     /db_xref="GeneID:117608869"
ORIGIN      
        1 mnarggyrcs fkgcpsasse ergiketlfr fpkdverskl wilacnreel lvktatqlys
       61 gyrvcklhfa tnmflnyert rlqphavpss ivrnndeess skslnveinc innlniqeen
      121 iaidifnesn qdnthidkti pnvsdvsevg tqtslslsst spqkahlyqq iraykrkian
      181 lesqlheakk rkedinslda lldkyfpqqt skylktqvrl fqkdakvl
//
LOCUS       XP_034190393             728 aa            linear   INV 03-SEP-2025
DEFINITION  phosphorylated CTD-interacting factor 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190393
VERSION     XP_034190393.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334502.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="phosphorylated CTD-interacting factor 1 isoform
                     X1"
                     /calculated_mol_wt=82553
     Region          105..132
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(118,129)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          459..627
                     /region_name="PCIF1_WW"
                     /note="Phosphorylated CTD interacting factor 1 WW domain;
                     pfam12237"
                     /db_xref="CDD:463502"
     CDS             1..728
                     /gene="Pcif1"
                     /coded_by="XM_034334502.2:274..2460"
                     /db_xref="GeneID:117608846"
ORIGIN      
        1 mnevsggkqd ipntstwetl pgqsaslstm hhhhqslqqd anptvtqviv ptpvklqapm
       61 lnstqtvadh csqlghmqqs nlitqgtppg tfaepelspe lqqqgwkkcw skrenrpyfw
      121 nkltgeslwv ipplkpqfdp itdplgicgv ppvagngaip sggtlkrras edsvvpaakk
      181 fvlagpwdle iptnviiyer apsnlphvhp etealrcgll aklrqcyqel chtresidap
      241 kdsfnrwlme rkvidcgsdp llpsqcfpei smsmyreimn dipiklvrpk ftgdarkqls
      301 ryaeaakkmi esraassesr kvvkwnaedt fqwlrrtvga tfddfqdrla hlkrqcqphl
      361 tetvkasveg iclkiyhlst eyakkvkhkn nqilkdnglg nviplggpas tqrkvwcypv
      421 qfslptsrlp qvdylpereq tmlrfhgdtl cinnmhlakl ehlyryncfd dkkfemflpr
      481 vwcmlkryqt ylginegqat qmalpvtvfe clqrsfgvsf ecfasplncy frqycsafad
      541 tdsyfgsrgp fldfrpvsgs fqanppycee lmeamvnhfe rlladstepl sfvvflpewr
      601 dpapnalikl esshfkrkqv vvpameheyr hgfqhilpkg evniraahgt lvvwlqntag
      661 tarwgpteer vealleawrp grererdrqe llspprqthq pipsmpipvl ttpttptvpl
      721 qtlsthpi
//
LOCUS       XP_034190394             699 aa            linear   INV 03-SEP-2025
DEFINITION  phosphorylated CTD-interacting factor 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190394
VERSION     XP_034190394.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334503.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..699
                     /product="phosphorylated CTD-interacting factor 1 isoform
                     X2"
                     /calculated_mol_wt=79535
     Region          76..103
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(89,100)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          430..598
                     /region_name="PCIF1_WW"
                     /note="Phosphorylated CTD interacting factor 1 WW domain;
                     pfam12237"
                     /db_xref="CDD:463502"
     CDS             1..699
                     /gene="Pcif1"
                     /coded_by="XM_034334503.2:328..2427"
                     /db_xref="GeneID:117608846"
ORIGIN      
        1 mhhhhqslqq danptvtqvi vptpvklqap mlnstqtvad hcsqlghmqq snlitqgtpp
       61 gtfaepelsp elqqqgwkkc wskrenrpyf wnkltgeslw vipplkpqfd pitdplgicg
      121 vppvagngai psggtlkrra sedsvvpaak kfvlagpwdl eiptnviiye rapsnlphvh
      181 petealrcgl laklrqcyqe lchtresida pkdsfnrwlm erkvidcgsd pllpsqcfpe
      241 ismsmyreim ndipiklvrp kftgdarkql sryaeaakkm iesraasses rkvvkwnaed
      301 tfqwlrrtvg atfddfqdrl ahlkrqcqph ltetvkasve giclkiyhls teyakkvkhk
      361 nnqilkdngl gnviplggpa stqrkvwcyp vqfslptsrl pqvdylpere qtmlrfhgdt
      421 lcinnmhlak lehlyryncf ddkkfemflp rvwcmlkryq tylginegqa tqmalpvtvf
      481 eclqrsfgvs fecfasplnc yfrqycsafa dtdsyfgsrg pfldfrpvsg sfqanppyce
      541 elmeamvnhf erlladstep lsfvvflpew rdpapnalik lesshfkrkq vvvpamehey
      601 rhgfqhilpk gevniraahg tlvvwlqnta gtarwgptee rvealleawr pgrererdrq
      661 ellspprqth qpipsmpipv lttpttptvp lqtlsthpi
//
LOCUS       XP_034190395             603 aa            linear   INV 03-SEP-2025
DEFINITION  phosphorylated CTD-interacting factor 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190395
VERSION     XP_034190395.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334504.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..603
                     /product="phosphorylated CTD-interacting factor 1 isoform
                     X3"
                     /calculated_mol_wt=68523
     Region          334..502
                     /region_name="PCIF1_WW"
                     /note="Phosphorylated CTD interacting factor 1 WW domain;
                     pfam12237"
                     /db_xref="CDD:463502"
     CDS             1..603
                     /gene="Pcif1"
                     /coded_by="XM_034334504.2:127..1938"
                     /db_xref="GeneID:117608846"
ORIGIN      
        1 mgnttfetsg tkfdpitdpl gicgvppvag ngaipsggtl krrasedsvv paakkfvlag
       61 pwdleiptnv iiyerapsnl phvhpeteal rcgllaklrq cyqelchtre sidapkdsfn
      121 rwlmerkvid cgsdpllpsq cfpeismsmy reimndipik lvrpkftgda rkqlsryaea
      181 akkmiesraa ssesrkvvkw naedtfqwlr rtvgatfddf qdrlahlkrq cqphltetvk
      241 asvegiclki yhlsteyakk vkhknnqilk dnglgnvipl ggpastqrkv wcypvqfslp
      301 tsrlpqvdyl pereqtmlrf hgdtlcinnm hlaklehlyr yncfddkkfe mflprvwcml
      361 kryqtylgin egqatqmalp vtvfeclqrs fgvsfecfas plncyfrqyc safadtdsyf
      421 gsrgpfldfr pvsgsfqanp pyceelmeam vnhferllad steplsfvvf lpewrdpapn
      481 aliklesshf krkqvvvpam eheyrhgfqh ilpkgevnir aahgtlvvwl qntagtarwg
      541 pteerveall eawrpgrere rdrqellspp rqthqpipsm pipvlttptt ptvplqtlst
      601 hpi
//
LOCUS       XP_034190454             419 aa            linear   INV 03-SEP-2025
DEFINITION  jumonji domain containing 4 [Osmia lignaria lignaria].
ACCESSION   XP_034190454
VERSION     XP_034190454.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334563.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..419
                     /product="jumonji domain containing 4"
                     /calculated_mol_wt=49394
     Region          35..278
                     /region_name="Cupin_8"
                     /note="Cupin-like domain; pfam13621"
                     /db_xref="CDD:463936"
     CDS             1..419
                     /gene="JMJD4"
                     /coded_by="XM_034334563.2:260..1519"
                     /db_xref="GeneID:117608859"
ORIGIN      
        1 mlreleiknn rlctekntai idwidyvnts itydeffsky inlnkpcifk snitenwscr
       61 rqwnldgapd fdvldilygn cvvpvadcnk kyynsqskdd memkdylnyw teyaksnysd
      121 smpllylkdw hcpklfpnap mynvpeyfas dwlneyyian pelnddyrfv ymgpkgtwtp
      181 lhadvfgsys wsanivgrkh wllfppgqed flrdihgqli ydatsdelnd ytkykaydkr
      241 alkyidviqa egeimfvpsg whhqvwnled tisvnhnwin gcnilnvwhg lkkelssvmk
      301 evndckdmhd waeqcqlilk stygmdyslf fdfltfiakk rlnvfsnnee aisfnkykfg
      361 nnhctfdlha iklvlidfit dmeeksiyhl icdknqghkl lnkillllqs hydaenist
//
LOCUS       XP_076545644             305 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305513 [Osmia lignaria lignaria].
ACCESSION   XP_076545644
VERSION     XP_076545644.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689529.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..305
                     /product="uncharacterized protein LOC143305513"
                     /calculated_mol_wt=34734
     CDS             1..305
                     /gene="LOC143305513"
                     /coded_by="XM_076689529.1:1651..2568"
                     /db_xref="GeneID:143305513"
ORIGIN      
        1 myfeklhnih liaadwrvtl sidisklktq fpleyselng vygkclsltt atacaatlrl
       61 phvygkadqa lslqdqlnei itefqtpesy dgpktirrrs apfgfigsis htlfgiltte
      121 daeyynseid klyhnqnela himenqahiv rnglhklhqi fekntelise nrnrlneite
      181 aiqqeensvn tlqymmalsr fvhsieisld hyiktaetyl aairavksge vphallgrsi
      241 srriisdiwn tqrnfdlpip pgqqslkelt rsttvnaiih qgrilivlql plsegttyhl
      301 yrtys
//
LOCUS       XP_034190419             555 aa            linear   INV 03-SEP-2025
DEFINITION  polycomb protein Scm isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190419
VERSION     XP_034190419.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334528.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..555
                     /product="polycomb protein Scm isoform X1"
                     /calculated_mol_wt=61172
     Region          17..53
                     /region_name="zf-FCS"
                     /note="MYM-type Zinc finger with FCS sequence motif;
                     pfam06467"
                     /db_xref="CDD:428958"
     Region          163..292
                     /region_name="MBT"
                     /note="malignant brain tumor (MBT) repeat; cl45897"
                     /db_xref="CDD:459242"
     Site            order(203,206,209,211,227,230,234)
                     /site_type="other"
                     /note="putative methyllysine peptide binding site
                     [polypeptide binding]"
                     /db_xref="CDD:439080"
     Region          297..370
                     /region_name="MBT_dScm-like_rpt2"
                     /note="second malignant brain tumor (MBT) repeat found in
                     the Polycomb protein sex comb on midleg family; cd20092"
                     /db_xref="CDD:439082"
     Site            order(312,315,317..318,320,336,339,343,369..370)
                     /site_type="other"
                     /note="methyllysine peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:439082"
     Region          370..>482
                     /region_name="PHA03255"
                     /note="BDLF3; Provisional"
                     /db_xref="CDD:165513"
     Region          479..549
                     /region_name="SAM_Scm"
                     /note="SAM domain of Scm proteins of Polycomb group;
                     cd09578"
                     /db_xref="CDD:188977"
     Site            order(498,533..536,538..539,542,545)
                     /site_type="other"
                     /note="oligomer interface EH [polypeptide binding]"
                     /db_xref="CDD:188977"
     Site            order(510..514,516..517,520..521,524..525,527..530)
                     /site_type="other"
                     /note="oligomer interface ML [polypeptide binding]"
                     /db_xref="CDD:188977"
     CDS             1..555
                     /gene="Scm"
                     /coded_by="XM_034334528.2:444..2111"
                     /db_xref="GeneID:117608851"
ORIGIN      
        1 msstqskmrg pgrppkskns ctwcgetkqp lkyvlptqhg kkefcsetcl sefrkayvrg
       61 acvqcdnvir gtpvrleqkd gptkdfcssf clnkhqkreg qvdmkknnrd hsspspsltp
      121 sglisgnnvp sttqsftstn htnishppst stgpfqyety qtfdwdlylk etnsqaapve
      181 cfkqhevppt nefkmgmkle aldprnltst ciatvvgvlg prlrlrldgs dnkndfwrlv
      241 dsneihpigh ceksggmlqp plgfrmnass wpmfllktln gaemapakvf krepktprsn
      301 mfevghklea idkknpqlic tatvgavkdd mihitfdgwr gafdywcrfd srdifpagwc
      361 fksghplqpp rqkstgpnrf ksrtsnilpv mavsgagtng epavalvspa gssappqpat
      421 epdtstantk shtiensmql dnstlsspta akqprksvpe leaatsttqs essinrtpsa
      481 epaewtiedv ihyigvtdpa lgqhadlfrk heidgkalll lnsdmmmkym glklgpalki
      541 cnlvnrikgr rhill
//
LOCUS       XP_034190420             553 aa            linear   INV 03-SEP-2025
DEFINITION  polycomb protein Scm isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190420
VERSION     XP_034190420.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334529.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="polycomb protein Scm isoform X2"
                     /calculated_mol_wt=61113
     Region          17..53
                     /region_name="zf-FCS"
                     /note="MYM-type Zinc finger with FCS sequence motif;
                     pfam06467"
                     /db_xref="CDD:428958"
     Region          163..292
                     /region_name="MBT"
                     /note="malignant brain tumor (MBT) repeat; cl45897"
                     /db_xref="CDD:459242"
     Site            order(203,206,209,211,227,230,234)
                     /site_type="other"
                     /note="putative methyllysine peptide binding site
                     [polypeptide binding]"
                     /db_xref="CDD:439080"
     Region          297..370
                     /region_name="MBT_dScm-like_rpt2"
                     /note="second malignant brain tumor (MBT) repeat found in
                     the Polycomb protein sex comb on midleg family; cd20092"
                     /db_xref="CDD:439082"
     Site            order(312,315,317..318,320,336,339,343,369..370)
                     /site_type="other"
                     /note="methyllysine peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:439082"
     Region          477..547
                     /region_name="SAM_Scm"
                     /note="SAM domain of Scm proteins of Polycomb group;
                     cd09578"
                     /db_xref="CDD:188977"
     Site            order(496,531..534,536..537,540,543)
                     /site_type="other"
                     /note="oligomer interface EH [polypeptide binding]"
                     /db_xref="CDD:188977"
     Site            order(508..512,514..515,518..519,522..523,525..528)
                     /site_type="other"
                     /note="oligomer interface ML [polypeptide binding]"
                     /db_xref="CDD:188977"
     CDS             1..553
                     /gene="Scm"
                     /coded_by="XM_034334529.2:447..2108"
                     /db_xref="GeneID:117608851"
ORIGIN      
        1 msstqskmrg pgrppkskns ctwcgetkqp lkyvlptqhg kkefcsetcl sefrkayvrg
       61 acvqcdnvir gtpvrleqkd gptkdfcssf clnkhqkreg qvdmkknnrd hsspspsltp
      121 sglisgnnvp sttqsftstn htnishppst stgpfqyety qtfdwdlylk etnsqaapve
      181 cfkqhevppt nefkmgmkle aldprnltst ciatvvgvlg prlrlrldgs dnkndfwrlv
      241 dsneihpigh ceksggmlqp plgfrmnass wpmfllktln gaemapakvf krepktprsn
      301 mfevghklea idkknpqlic tatvgavkdd mihitfdgwr gafdywcrfd srdifpagwc
      361 fksghplqpp rqkwpnrfks rtsnilpvma vsgagtngep avalvspags sappqpatep
      421 dtstantksh tiensmqldn stlssptaak qprksvpele aatsttqses sinrtpsaep
      481 aewtiedvih yigvtdpalg qhadlfrkhe idgkalllln sdmmmkymgl klgpalkicn
      541 lvnrikgrrh ill
//
LOCUS       XP_034190487             215 aa            linear   INV 03-SEP-2025
DEFINITION  phosducin-like protein 3 [Osmia lignaria lignaria].
ACCESSION   XP_034190487
VERSION     XP_034190487.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334596.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190487.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="phosducin-like protein 3"
                     /calculated_mol_wt=24730
     Region          60..171
                     /region_name="Phd_like_TxnDC9"
                     /note="Phosducin (Phd)-like family, Thioredoxin (TRX)
                     domain containing protein 9 (TxnDC9) subfamily; composed
                     of predominantly uncharacterized eukaryotic proteins,
                     containing a TRX-like domain without the redox active CXXC
                     motif. The gene name for the human...; cd02989"
                     /db_xref="CDD:239287"
     CDS             1..215
                     /gene="LOC117608870"
                     /coded_by="XM_034334596.2:163..810"
                     /db_xref="GeneID:117608870"
ORIGIN      
        1 meavlqqkvi eatqcvekql daeiekldnl disdieklre qrlkklkllq qqkqnwlslg
       61 hgeyselqde keffeiskks enivclfykd dserskivdh hfkilakkhi eakfcklnvm
      121 rcpflterlr ikiiptiaii vngktkdyiv gftdlgnrdd fstetlelri slagaitved
      181 ncpseinkkp wlsytpkskt irgpndsnsd dsddd
//
LOCUS       XP_034192245            1063 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192245
VERSION     XP_034192245.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336354.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192245.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1063
                     /product="uncharacterized protein LOC117609725 isoform X1"
                     /calculated_mol_wt=121877
     Region          933..1021
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1063
                     /gene="LOC117609725"
                     /coded_by="XM_034336354.2:440..3631"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnsey esqcsdsenv
      421 vsikgdsqql fycyteyekq sfsshlnknv rkskeclten slkkdsgles gevsdnseeq
      481 svitivngvq tnesvkqiqn rikecqkets skqgnyqhsd tstnisnfvv nkvqsksanv
      541 pkhemkiqsa latsilqlnq gvlsktesvt tgnqrmkqmv svlkkppnvq qspvlittsn
      601 esvvtttnsl ndqvqniivq teektfvqqn nkkpprkkln laeyrsrreq nrsdnsrtns
      661 pvspmtlvyi hhastttepi kddpqnpiws ereivsllkp kaeidekkvl pkpstcdvgi
      721 qtyetvfefp qnaligider ersireksqq pckkqrisss ssrsrsrsns ksksrsrsrs
      781 ssnrsrsykr nntrhrrish rrssvsssss wssrsrsrsr srsrsrsntr sslssessys
      841 rsrsrlsrsr srsssryssc srssshsnfg rnrwsrrkrr qgkkyhrsyd rdsqhstnsy
      901 rdygdwrsrs ssssyrrpyd hwydrekqrq veerrviyvg rleegitkad lrrrfevfgp
      961 vvdisvhfre hgdnygfvtf aykndayeav ehgndnptlp rydlcfggrr afckvkyadl
     1021 dmtlfftdgm psdsfgselq snedntfdll lkeakaklrk rkv
//
LOCUS       XP_034192246            1063 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192246
VERSION     XP_034192246.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192246.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1063
                     /product="uncharacterized protein LOC117609725 isoform X1"
                     /calculated_mol_wt=121877
     Region          933..1021
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1063
                     /gene="LOC117609725"
                     /coded_by="XM_034336355.2:311..3502"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnsey esqcsdsenv
      421 vsikgdsqql fycyteyekq sfsshlnknv rkskeclten slkkdsgles gevsdnseeq
      481 svitivngvq tnesvkqiqn rikecqkets skqgnyqhsd tstnisnfvv nkvqsksanv
      541 pkhemkiqsa latsilqlnq gvlsktesvt tgnqrmkqmv svlkkppnvq qspvlittsn
      601 esvvtttnsl ndqvqniivq teektfvqqn nkkpprkkln laeyrsrreq nrsdnsrtns
      661 pvspmtlvyi hhastttepi kddpqnpiws ereivsllkp kaeidekkvl pkpstcdvgi
      721 qtyetvfefp qnaligider ersireksqq pckkqrisss ssrsrsrsns ksksrsrsrs
      781 ssnrsrsykr nntrhrrish rrssvsssss wssrsrsrsr srsrsrsntr sslssessys
      841 rsrsrlsrsr srsssryssc srssshsnfg rnrwsrrkrr qgkkyhrsyd rdsqhstnsy
      901 rdygdwrsrs ssssyrrpyd hwydrekqrq veerrviyvg rleegitkad lrrrfevfgp
      961 vvdisvhfre hgdnygfvtf aykndayeav ehgndnptlp rydlcfggrr afckvkyadl
     1021 dmtlfftdgm psdsfgselq snedntfdll lkeakaklrk rkv
//
LOCUS       XP_034192247            1063 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192247
VERSION     XP_034192247.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336356.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192247.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1063
                     /product="uncharacterized protein LOC117609725 isoform X1"
                     /calculated_mol_wt=121877
     Region          933..1021
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1063
                     /gene="LOC117609725"
                     /coded_by="XM_034336356.2:100..3291"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnsey esqcsdsenv
      421 vsikgdsqql fycyteyekq sfsshlnknv rkskeclten slkkdsgles gevsdnseeq
      481 svitivngvq tnesvkqiqn rikecqkets skqgnyqhsd tstnisnfvv nkvqsksanv
      541 pkhemkiqsa latsilqlnq gvlsktesvt tgnqrmkqmv svlkkppnvq qspvlittsn
      601 esvvtttnsl ndqvqniivq teektfvqqn nkkpprkkln laeyrsrreq nrsdnsrtns
      661 pvspmtlvyi hhastttepi kddpqnpiws ereivsllkp kaeidekkvl pkpstcdvgi
      721 qtyetvfefp qnaligider ersireksqq pckkqrisss ssrsrsrsns ksksrsrsrs
      781 ssnrsrsykr nntrhrrish rrssvsssss wssrsrsrsr srsrsrsntr sslssessys
      841 rsrsrlsrsr srsssryssc srssshsnfg rnrwsrrkrr qgkkyhrsyd rdsqhstnsy
      901 rdygdwrsrs ssssyrrpyd hwydrekqrq veerrviyvg rleegitkad lrrrfevfgp
      961 vvdisvhfre hgdnygfvtf aykndayeav ehgndnptlp rydlcfggrr afckvkyadl
     1021 dmtlfftdgm psdsfgselq snedntfdll lkeakaklrk rkv
//
LOCUS       XP_034192248            1062 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192248
VERSION     XP_034192248.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336357.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192248.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1062
                     /product="uncharacterized protein LOC117609725 isoform X2"
                     /calculated_mol_wt=121790
     Region          932..1020
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1062
                     /gene="LOC117609725"
                     /coded_by="XM_034336357.2:441..3629"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnsey esqcsdsenv
      421 vsikgdsqql fycyteyekq sfsshlnknv rkskeclten slkkdsgles gevsdnseeq
      481 svitivngvq tnesvkqiqn rikecqkets skqgnyqhsd tstnisnfvv nkvqsksanv
      541 pkhemkiqsa latsilqlnq gvlsktesvt tgnqrmkqmv svlkkppnvq qspvlittsn
      601 esvvtttnsl ndqvqniivq teektfvqqn nkkpprkkln laeyrsrreq nrsdnsrtns
      661 pvspmtlvyi hhastttepi kddpqnpiws ereivsllkp kaeidekkvl pkpstcdvgi
      721 qtyetvfefp qnaligider ersireksqq pckkqrisss ssrsrsrsns ksksrsrsrs
      781 ssnrsrsykr nntrhrrish rrssvsssss wssrsrsrsr srsrsrsntr sslssessys
      841 rsrsrlsrsr srsssryssc srssshsnfg rnrwsrrkrr qgkkyhrsyd rdsqhstnsy
      901 rdygdwrrss sssyrrpydh wydrekqrqv eerrviyvgr leegitkadl rrrfevfgpv
      961 vdisvhfreh gdnygfvtfa ykndayeave hgndnptlpr ydlcfggrra fckvkyadld
     1021 mtlfftdgmp sdsfgselqs nedntfdlll keakaklrkr kv
//
LOCUS       XP_034192249            1056 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192249
VERSION     XP_034192249.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336358.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192249.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1056
                     /product="uncharacterized protein LOC117609725 isoform X3"
                     /calculated_mol_wt=121021
     Region          933..1021
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1056
                     /gene="LOC117609725"
                     /coded_by="XM_034336358.2:440..3610"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnsey esqcsdsenv
      421 vsikgdsqql fycyteyekq sfsshlnknv rkskeclten slkkdsgles gevsdnseeq
      481 svitivngvq tnesvkqiqn rikecqkets skqgnyqhsd tstnisnfvv nkvqsksanv
      541 pkhemkiqsa latsilqlnq gvlsktesvt tgnqrmkqmv svlkkppnvq qspvlittsn
      601 esvvtttnsl ndqvqniivq teektfvqqn nkkpprkkln laeyrsrreq nrsdnsrtns
      661 pvspmtlvyi hhastttepi kddpqnpiws ereivsllkp kaeidekkvl pkpstcdvgi
      721 qtyetvfefp qnaligider ersireksqq pckkqrisss ssrsrsrsns ksksrsrsrs
      781 ssnrsrsykr nntrhrrish rrssvsssss wssrsrsrsr srsrsrsntr sslssessys
      841 rsrsrlsrsr srsssryssc srssshsnfg rnrwsrrkrr qgkkyhrsyd rdsqhstnsy
      901 rdygdwrsrs ssssyrrpyd hwydrekqrq veerrviyvg rleegitkad lrrrfevfgp
      961 vvdisvhfre hgdnygfvtf aykndayeav ehgndnptlp rydlcfggrr afckvkyadl
     1021 dgmpsdsfgs elqsnedntf dlllkeakak lrkrkv
//
LOCUS       XP_076545645            1055 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545645
VERSION     XP_076545645.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689530.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1055
                     /product="uncharacterized protein LOC117609725 isoform X4"
                     /calculated_mol_wt=120934
     Region          932..1020
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1055
                     /gene="LOC117609725"
                     /coded_by="XM_076689530.1:440..3607"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnsey esqcsdsenv
      421 vsikgdsqql fycyteyekq sfsshlnknv rkskeclten slkkdsgles gevsdnseeq
      481 svitivngvq tnesvkqiqn rikecqkets skqgnyqhsd tstnisnfvv nkvqsksanv
      541 pkhemkiqsa latsilqlnq gvlsktesvt tgnqrmkqmv svlkkppnvq qspvlittsn
      601 esvvtttnsl ndqvqniivq teektfvqqn nkkpprkkln laeyrsrreq nrsdnsrtns
      661 pvspmtlvyi hhastttepi kddpqnpiws ereivsllkp kaeidekkvl pkpstcdvgi
      721 qtyetvfefp qnaligider ersireksqq pckkqrisss ssrsrsrsns ksksrsrsrs
      781 ssnrsrsykr nntrhrrish rrssvsssss wssrsrsrsr srsrsrsntr sslssessys
      841 rsrsrlsrsr srsssryssc srssshsnfg rnrwsrrkrr qgkkyhrsyd rdsqhstnsy
      901 rdygdwrrss sssyrrpydh wydrekqrqv eerrviyvgr leegitkadl rrrfevfgpv
      961 vdisvhfreh gdnygfvtfa ykndayeave hgndnptlpr ydlcfggrra fckvkyadld
     1021 gmpsdsfgse lqsnedntfd lllkeakakl rkrkv
//
LOCUS       XP_076545646            1035 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545646
VERSION     XP_076545646.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689531.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1035
                     /product="uncharacterized protein LOC117609725 isoform X5"
                     /calculated_mol_wt=118684
     Region          905..993
                     /region_name="RRM_PPARGC1A_like"
                     /note="RNA recognition motif (RRM) found in the peroxisome
                     proliferator-activated receptor gamma coactivator 1A
                     (PGC-1alpha) family of regulated coactivators; cd12357"
                     /db_xref="CDD:409793"
     CDS             1..1035
                     /gene="LOC117609725"
                     /coded_by="XM_076689531.1:440..3547"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnkye kqsfsshlnk
      421 nvrkskeclt enslkkdsgl esgevsdnse eqsvitivng vqtnesvkqi qnrikecqke
      481 tsskqgnyqh sdtstnisnf vvnkvqsksa nvpkhemkiq salatsilql nqgvlsktes
      541 vttgnqrmkq mvsvlkkppn vqqspvlitt snesvvtttn slndqvqnii vqteektfvq
      601 qnnkkpprkk lnlaeyrsrr eqnrsdnsrt nspvspmtlv yihhasttte pikddpqnpi
      661 wsereivsll kpkaeidekk vlpkpstcdv giqtyetvfe fpqnaligid erersireks
      721 qqpckkqris ssssrsrsrs nsksksrsrs rsssnrsrsy krnntrhrri shrrssvsss
      781 sswssrsrsr srsrsrsrsn trsslssess ysrsrsrlsr srsrsssrys scsrssshsn
      841 fgrnrwsrrk rrqgkkyhrs ydrdsqhstn syrdygdwrs rsssssyrrp ydhwydrekq
      901 rqveerrviy vgrleegitk adlrrrfevf gpvvdisvhf rehgdnygfv tfaykndaye
      961 avehgndnpt lprydlcfgg rrafckvkya dldmtlfftd gmpsdsfgse lqsnedntfd
     1021 lllkeakakl rkrkv
//
LOCUS       XP_034192255            1027 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609725 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192255
VERSION     XP_034192255.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336364.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192255.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1027
                     /product="uncharacterized protein LOC117609725 isoform X6"
                     /calculated_mol_wt=117741
     Region          904..992
                     /region_name="RRM_PPARGC1A_like"
                     /note="RNA recognition motif (RRM) found in the peroxisome
                     proliferator-activated receptor gamma coactivator 1A
                     (PGC-1alpha) family of regulated coactivators; cd12357"
                     /db_xref="CDD:409793"
     CDS             1..1027
                     /gene="LOC117609725"
                     /coded_by="XM_034336364.2:440..3523"
                     /db_xref="GeneID:117609725"
ORIGIN      
        1 meteflnfhd eghfsdfsls leqsvvekpq dllmhmvend fdymethldi tnsdglwnnq
       61 hecddgvget silensklds ikedfakvlt dwqehigylq ssdmdeymdi igfnintidk
      121 knfctsenvl qdelihtves dflvankedl ekeshkishn ildleisdnk tendnkqkev
      181 esasllkepk cakgqskmkk nvskykniri dinatqkkkr kekqlrynkd thnsddnsdk
      241 ssvnqlpvlq agdvkslleq feasentmsf ysssninter dqevcvkske tqqhmkqsht
      301 ftpkkslsri snlhknirsa lpkemvekik tsahkkgisi istipnaqsn grsngtrmqd
      361 aavtlfrnkl lkvvtndnkn ksinggliql dhdycstnss isssdsnkye kqsfsshlnk
      421 nvrkskeclt enslkkdsgl esgevsdnse eqsvitivng vqtnesvkqi qnrikecqke
      481 tsskqgnyqh sdtstnisnf vvnkvqsksa nvpkhemkiq salatsilql nqgvlsktes
      541 vttgnqrmkq mvsvlkkppn vqqspvlitt snesvvtttn slndqvqnii vqteektfvq
      601 qnnkkpprkk lnlaeyrsrr eqnrsdnsrt nspvspmtlv yihhasttte pikddpqnpi
      661 wsereivsll kpkaeidekk vlpkpstcdv giqtyetvfe fpqnaligid erersireks
      721 qqpckkqris ssssrsrsrs nsksksrsrs rsssnrsrsy krnntrhrri shrrssvsss
      781 sswssrsrsr srsrsrsrsn trsslssess ysrsrsrlsr srsrsssrys scsrssshsn
      841 fgrnrwsrrk rrqgkkyhrs ydrdsqhstn syrdygdwrr sssssyrrpy dhwydrekqr
      901 qveerrviyv grleegitka dlrrrfevfg pvvdisvhfr ehgdnygfvt faykndayea
      961 vehgndnptl prydlcfggr rafckvkyad ldgmpsdsfg selqsnednt fdlllkeaka
     1021 klrkrkv
//
LOCUS       XP_034184725             585 aa            linear   INV 03-SEP-2025
DEFINITION  bHLH protein similar to Deadpan [Osmia lignaria lignaria].
ACCESSION   XP_034184725
VERSION     XP_034184725.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328834.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184725.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="bHLH protein similar to Deadpan"
                     /calculated_mol_wt=62890
     Region          65..116
                     /region_name="bHLH_O_HES"
                     /note="basic helix-loop-helix-orange (bHLH-O) domain found
                     in hairy and enhancer of split (HES) family; cd11410"
                     /db_xref="CDD:381416"
     Site            order(67,69..70,74,99..100)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381416"
     Site            order(69..70,72..74,76..77,79..80,83..84,87,97,99..101,
                     104..105,107..108,110..112,114..115)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381416"
     Region          124..170
                     /region_name="Hairy_orange"
                     /note="Hairy Orange; pfam07527"
                     /db_xref="CDD:462193"
     CDS             1..585
                     /gene="Sidpn"
                     /coded_by="XM_034328834.2:337..2094"
                     /db_xref="GeneID:117606427"
ORIGIN      
        1 mtarlhslrh qekklagnsh rqhhhqqsqn qqsaqqgasp aaspspslsp spkhsdgrra
       61 nkplmekrrr arinqslaal kalildsarl entkhsklek adileltvrh lqrqrslaqp
      121 glsrykagyq dcsrevsryl dapdiitgnt tpmdpavkqr llrhldscvs eldldlgsrp
      181 dsglgsspgs vtdrvtgass pgplehhvps vsrsstalnp tglikaeipd ieparpdsst
      241 tggdennnns ssgsnsnngp stafgqvnst gnldpppppp phhhhhhhhh hqppsvptps
      301 gipvidqpss sqqnpnmlsv vqvipsrlpd gqvvfllpsh yvqlaaaaaa nginigpnpp
      361 ttvwaatnms flkatdklak rhhedmqewq davssvaana tvanvttavt agitvaatat
      421 viataaaaat atattsvnas vsasisasap vstfvtdnll sasksnngvp kveqteqpld
      481 ftttskklks gtkstlpsvl qhhlhhhhhh hhhhqqqqqq qqqqqqfttm ttttttarls
      541 iegslepeer sfrrannefv tgirspsphp hqqpikdeeg mwrpw
//
LOCUS       XP_076545647             418 aa            linear   INV 03-SEP-2025
DEFINITION  carbohydrate sulfotransferase 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545647
VERSION     XP_076545647.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689532.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..418
                     /product="carbohydrate sulfotransferase 4 isoform X1"
                     /calculated_mol_wt=48643
     Region          124..395
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..418
                     /gene="LOC117606807"
                     /coded_by="XM_076689532.1:404..1660"
                     /db_xref="GeneID:117606807"
ORIGIN      
        1 mskrlsfygl vglaslciff lafnqryssy lehrlqpvis vskksedvei rphiiriqdp
       61 vtayfrgsie nitlseiqev vdqqrnaien emedykypng kygvnvskle dlvlekggrp
      121 mrsiiltswr sgstflgdvv nahpanfyhy eplldfgivq irgppladka lknlesllrc
      181 dyfnldryld ygkthswvfn hnnrlwkqcq ihkkicwdpr fvskfcklfp fqsmkivrlr
      241 lraaekllkq edlgvrlvll irdprgilqs rkhrewcptn pdcsdpalvc admvsdfsaa
      301 vrlikkypht frvvryedls vdpykhvkel ynfygldfhi nvkkfldtht kndvggvsst
      361 frnskvapfh wrtdldfeev deiqrvcaaa mrlwgyvmas nsshqkefdp ltayqlkl
//
LOCUS       XP_034185620             412 aa            linear   INV 03-SEP-2025
DEFINITION  carbohydrate sulfotransferase 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185620
VERSION     XP_034185620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..412
                     /product="carbohydrate sulfotransferase 4 isoform X2"
                     /calculated_mol_wt=47984
     Region          118..389
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..412
                     /gene="LOC117606807"
                     /coded_by="XM_034329729.2:403..1641"
                     /db_xref="GeneID:117606807"
ORIGIN      
        1 mskrlsfygl vglaslciff lafnqryssy lehrlqpvis dveirphiir iqdpvtayfr
       61 gsienitlse iqevvdqqrn aienemedyk ypngkygvnv skledlvlek ggrpmrsiil
      121 tswrsgstfl gdvvnahpan fyhyeplldf givqirgppl adkalknles llrcdyfnld
      181 ryldygkths wvfnhnnrlw kqcqihkkic wdprfvskfc klfpfqsmki vrlrlraaek
      241 llkqedlgvr lvllirdprg ilqsrkhrew cptnpdcsdp alvcadmvsd fsaavrlikk
      301 yphtfrvvry edlsvdpykh vkelynfygl dfhinvkkfl dthtkndvgg vsstfrnskv
      361 apfhwrtdld feevdeiqrv caaamrlwgy vmasnsshqk efdpltayql kl
//
LOCUS       XP_034185621             402 aa            linear   INV 03-SEP-2025
DEFINITION  carbohydrate sulfotransferase 4 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185621
VERSION     XP_034185621.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329730.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="carbohydrate sulfotransferase 4 isoform X3"
                     /calculated_mol_wt=46760
     Region          108..379
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..402
                     /gene="LOC117606807"
                     /coded_by="XM_034329730.2:403..1611"
                     /db_xref="GeneID:117606807"
ORIGIN      
        1 mskrlsfygl vglaslciff lafnqryssy lehrlqpvis vskkseayfr gsienitlse
       61 iqevvdqqrn aienemedyk ypngkygvnv skledlvlek ggrpmrsiil tswrsgstfl
      121 gdvvnahpan fyhyeplldf givqirgppl adkalknles llrcdyfnld ryldygkths
      181 wvfnhnnrlw kqcqihkkic wdprfvskfc klfpfqsmki vrlrlraaek llkqedlgvr
      241 lvllirdprg ilqsrkhrew cptnpdcsdp alvcadmvsd fsaavrlikk yphtfrvvry
      301 edlsvdpykh vkelynfygl dfhinvkkfl dthtkndvgg vsstfrnskv apfhwrtdld
      361 feevdeiqrv caaamrlwgy vmasnsshqk efdpltayql kl
//
LOCUS       XP_034185624             396 aa            linear   INV 03-SEP-2025
DEFINITION  carbohydrate sulfotransferase 4 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185624
VERSION     XP_034185624.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329733.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="carbohydrate sulfotransferase 4 isoform X4"
                     /calculated_mol_wt=46101
     Region          102..373
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..396
                     /gene="LOC117606807"
                     /coded_by="XM_034329733.2:402..1592"
                     /db_xref="GeneID:117606807"
ORIGIN      
        1 mskrlsfygl vglaslciff lafnqryssy lehrlqpvis ayfrgsieni tlseiqevvd
       61 qqrnaienem edykypngky gvnvskledl vlekggrpmr siiltswrsg stflgdvvna
      121 hpanfyhyep lldfgivqir gppladkalk nlesllrcdy fnldryldyg kthswvfnhn
      181 nrlwkqcqih kkicwdprfv skfcklfpfq smkivrlrlr aaekllkqed lgvrlvllir
      241 dprgilqsrk hrewcptnpd csdpalvcad mvsdfsaavr likkyphtfr vvryedlsvd
      301 pykhvkelyn fygldfhinv kkfldthtkn dvggvsstfr nskvapfhwr tdldfeevde
      361 iqrvcaaamr lwgyvmasns shqkefdplt ayqlkl
//
LOCUS       XP_034185625             373 aa            linear   INV 03-SEP-2025
DEFINITION  carbohydrate sulfotransferase 4 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185625
VERSION     XP_034185625.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329734.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="carbohydrate sulfotransferase 4 isoform X5"
                     /calculated_mol_wt=43477
     Region          79..350
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..373
                     /gene="LOC117606807"
                     /coded_by="XM_034329734.2:186..1307"
                     /db_xref="GeneID:117606807"
ORIGIN      
        1 mdveirphii riqdpvtayf rgsienitls eiqevvdqqr naienemedy kypngkygvn
       61 vskledlvle kggrpmrsii ltswrsgstf lgdvvnahpa nfyhyeplld fgivqirgpp
      121 ladkalknle sllrcdyfnl dryldygkth swvfnhnnrl wkqcqihkki cwdprfvskf
      181 cklfpfqsmk ivrlrlraae kllkqedlgv rlvllirdpr gilqsrkhre wcptnpdcsd
      241 palvcadmvs dfsaavrlik kyphtfrvvr yedlsvdpyk hvkelynfyg ldfhinvkkf
      301 ldthtkndvg gvsstfrnsk vapfhwrtdl dfeevdeiqr vcaaamrlwg yvmasnsshq
      361 kefdpltayq lkl
//
LOCUS       XP_034185626             357 aa            linear   INV 03-SEP-2025
DEFINITION  carbohydrate sulfotransferase 4 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185626
VERSION     XP_034185626.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329735.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..357
                     /product="carbohydrate sulfotransferase 4 isoform X6"
                     /calculated_mol_wt=41594
     Region          63..334
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     CDS             1..357
                     /gene="LOC117606807"
                     /coded_by="XM_034329735.2:186..1259"
                     /db_xref="GeneID:117606807"
ORIGIN      
        1 mayfrgsien itlseiqevv dqqrnaiene medykypngk ygvnvskled lvlekggrpm
       61 rsiiltswrs gstflgdvvn ahpanfyhye plldfgivqi rgppladkal knlesllrcd
      121 yfnldryldy gkthswvfnh nnrlwkqcqi hkkicwdprf vskfcklfpf qsmkivrlrl
      181 raaekllkqe dlgvrlvlli rdprgilqsr khrewcptnp dcsdpalvca dmvsdfsaav
      241 rlikkyphtf rvvryedlsv dpykhvkely nfygldfhin vkkfldthtk ndvggvsstf
      301 rnskvapfhw rtdldfeevd eiqrvcaaam rlwgyvmasn sshqkefdpl tayqlkl
//
LOCUS       XP_034184977             416 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606534 [Osmia lignaria lignaria].
ACCESSION   XP_034184977
VERSION     XP_034184977.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329086.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184977.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="uncharacterized protein LOC117606534"
                     /calculated_mol_wt=47758
     CDS             1..416
                     /gene="LOC117606534"
                     /coded_by="XM_034329086.2:272..1522"
                     /db_xref="GeneID:117606534"
ORIGIN      
        1 mferisrall pclfailvsg sptfyqdyqe vrnqrhascp scrrerpdal vswysepvrr
       61 eflplepevd qwpaeqqvqp dplyynpydr avrsrgsppf rsladaskre npfafqrdit
      121 fdlapaptap vrglddpald sqppyqvsrn lgmtnymkpn lyntisrqsd latpnrysdy
      181 glsyeepsfg gfglsnsyed tnkkpyqayr sgrypdhsfg verplvqplp ypqrptpnrr
      241 vpvgprapdm eqffptrreq pqsspmiysp qvrnnpynrs pdlnvyktaq nrgrdaaevp
      301 ndsgfrdqmr eqerqelerr qreefenqqr vrmaqmnqgn ggrqsnghfs sngddqqehv
      361 ignrdavnen tnvevlgksk vtwnrltkps tevslrdtei vttperevtt ktpiaa
//
LOCUS       XP_034184978             222 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606535 [Osmia lignaria lignaria].
ACCESSION   XP_034184978
VERSION     XP_034184978.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329087.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="uncharacterized protein LOC117606535"
                     /calculated_mol_wt=24574
     Region          24..>100
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            42
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..222
                     /gene="LOC117606535"
                     /coded_by="XM_034329087.2:1450..2118"
                     /db_xref="GeneID:117606535"
ORIGIN      
        1 mgvpnskrqd tdddiiskks lqavkqiisf lkehareegl frragrrelr trilnslkkg
       61 ekphldnnnn aalecaaalq lflshlkkpv mpqhvqqlll adnpgvtahv iardalglir
      121 qdvggrhgel larlldllrh ltlsgppser selrgsplpi allpvfftlt padlvkwkqv
      181 aarfselite apeqlqlnen rsydvestgv tsgseanvrl er
//
LOCUS       XP_034184979             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606536 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184979
VERSION     XP_034184979.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329088.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC117606536 isoform X1"
                     /calculated_mol_wt=16323
     CDS             1..150
                     /gene="LOC117606536"
                     /coded_by="XM_034329088.2:231..683"
                     /db_xref="GeneID:117606536"
ORIGIN      
        1 mpilyvvtnl fqfvlfvlft avltavaats llmevtithf vhpmttfmlh qsgrlavslv
       61 dqsarctsha fnkmcemdhv qlqsaldgis metpevryar saipglveet qpviidigst
      121 eeqetastap tevtigdstm qeeeidettg
//
LOCUS       XP_034184980             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606536 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184980
VERSION     XP_034184980.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329089.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC117606536 isoform X2"
                     /calculated_mol_wt=16373
     CDS             1..150
                     /gene="LOC117606536"
                     /coded_by="XM_034329089.2:119..571"
                     /db_xref="GeneID:117606536"
ORIGIN      
        1 mfyllvvtnl fqfvlfvlft avltavaats llmevtithf vhpmttfmlh qsgrlavslv
       61 dqsarctsha fnkmcemdhv qlqsaldgis metpevryar saipglveet qpviidigst
      121 eeqetastap tevtigdstm qeeeidettg
//
LOCUS       XP_034184981             102 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606537 [Osmia lignaria lignaria].
ACCESSION   XP_034184981
VERSION     XP_034184981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="uncharacterized protein LOC117606537"
                     /calculated_mol_wt=11479
     CDS             1..102
                     /gene="LOC117606537"
                     /coded_by="XM_034329090.2:300..608"
                     /db_xref="GeneID:117606537"
ORIGIN      
        1 mdfqviladl ntklpclqhi lrpeyapyln tagtvllgwl iiswisylvw mlfapltvtv
       61 iaiilispmt akwclkqtvp gletifnefa ekfqtvlsrv rg
//
LOCUS       XP_034190455             366 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608860 [Osmia lignaria lignaria].
ACCESSION   XP_034190455
VERSION     XP_034190455.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334564.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="uncharacterized protein LOC117608860"
                     /calculated_mol_wt=41003
     Region          1..110
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(18,36..37,40,59..60,62..63,66,73,83..85,87)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410788"
     CDS             1..366
                     /gene="LOC117608860"
                     /coded_by="XM_034334564.2:836..1936"
                     /db_xref="GeneID:117608860"
ORIGIN      
        1 mprpsrdtyg dqkppysyis ltamaiwssr dkmlplaeiy kfiadrfpyy rkdtrrwqns
       61 lrhnlsfndc fikvprgphr pgkgaywalh paalsmfeng sllrrrkrfk lhkpdkellk
      121 selqalasam pppphsessp viainpsaqt ngltvanlhr lrddllrwel herrlgmtsg
      181 gnsspgfsap eagssyylls pevrqrlagt deilrgyenn llqtgswsfp gfqvspyvsq
      241 lsyfqtdipd srqicpgate tsisdktesr pfldtggrss nsepdgklrs ssileagiss
      301 qtsiqpeqqk kkkkpftien iiapddqqms gnmedekrgh llvprplyag fpfglstakv
      361 pyetat
//
LOCUS       XP_034184983             749 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606539 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184983
VERSION     XP_034184983.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329092.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184983.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..749
                     /product="uncharacterized protein LOC117606539 isoform X1"
                     /calculated_mol_wt=87393
     CDS             1..749
                     /gene="LOC117606539"
                     /coded_by="XM_034329092.2:153..2402"
                     /db_xref="GeneID:117606539"
ORIGIN      
        1 mpeqkvlrlq dvwrtcnkir aaifrigfnl wdyykpldpe kncliseskf isvlagplkd
       61 vvglsdreic dladyfrvqd grilytqfct iihdsvpefd knkplvtgle wedpehvnrl
      121 spaeyrklcl ilsqiavivn krrlilrpyf qdyeliakna gtvtfthfgr ilkflnivla
      181 seefsllikr fakdaytvny vaflqavdev qsyfdkhgml slsgelldqf pgrvitaelp
      241 klprpeigkl mpskvfgkqt vfhpvmeepr admplmkiiq qiqrhilenr iriseffkrf
      301 dhlnvgrvtv sqfkrgldgl qvsslgrlyl aeteidalve iykdpndpdr vcwrtfqddi
      361 dqvftvkele klpnlriesp pkeiadlkrr gtadwqcvpg sirdlceetl qkvrhriner
      421 rilikqffkd ydrnnkghvs raqfrqvlat avvllseeee yaleqrynde lgfnyvlflk
      481 eleaikieep lyksmleekm kinaekpppe gsvdeknivl ilakikakiv rervkvdcra
      541 rsfffkrfnl illfyakaie fmrpydprne qvikkvdfmr gldqlrcnls cteigtimkv
      601 fqspfrenyv dyvrfgevve eavtvgsler apllvpvqhv ppeaspktfl tfderhmatv
      661 amdklsrmqr pnllelfkdy dkenigtvsk nclikalsvr hmfqlisnre ldavykcfsv
      721 ekggqleiny raflealhlm renrkylpf
//
LOCUS       XP_034184984             727 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606539 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184984
VERSION     XP_034184984.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329093.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184984.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..727
                     /product="uncharacterized protein LOC117606539 isoform X2"
                     /calculated_mol_wt=84656
     Region          424..448
                     /region_name="EF-hand_6"
                     /note="EF-hand domain; pfam13405"
                     /db_xref="CDD:463869"
     CDS             1..727
                     /gene="LOC117606539"
                     /coded_by="XM_034329093.2:169..2352"
                     /db_xref="GeneID:117606539"
ORIGIN      
        1 mpeqkvlrlq dvwrtcnkir aaifrigfnl wdyykpldpe kncliseskf isvlagplkd
       61 vvglsdreic dladyfrvqd grilytqfct iihdsvpefd knkplvtgle wedpehvnrl
      121 spaeyrklcl ilsqiavivn krrlilrpyf qdyeliakna gtvtfthfgr ilkflnivla
      181 seefsllikr fakdaytvny vaflqavdev qsyfdkhgml slsgelldqf pgrvitaelp
      241 klprpeigkl mpskvfgkqt vfhpvmeepr admplmkiiq qiqrhilenr iriseffkrf
      301 dhlnvgrvtv sqfkrgldgl qvsslgrlyl aeteidalve iykdpndpdr vcwrtfqddi
      361 dqvftvkele klpnlriesp pkeiadlkrr gtadwqcvpg sirdlceetl qkvrhriner
      421 rilikqffkd ydrnnkghvs raqfrqvlat avvllseeee yaleqrynde lgfnyvlflk
      481 eleaikieep lyksmleekm kinaekpppe gsvdeknivl ilakikakiv rervkaiefm
      541 rpydprneqv ikkvdfmrgl dqlrcnlsct eigtimkvfq spfrenyvdy vrfgevveea
      601 vtvgslerap llvpvqhvpp easpktfltf derhmatvam dklsrmqrpn llelfkdydk
      661 enigtvsknc likalsvrhm fqlisnreld avykcfsvek ggqleinyra flealhlmre
      721 nrkylpf
//
LOCUS       XP_034184985             685 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606539 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184985
VERSION     XP_034184985.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329094.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184985.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..685
                     /product="uncharacterized protein LOC117606539 isoform X3"
                     /calculated_mol_wt=79784
     Region          360..384
                     /region_name="EF-hand_6"
                     /note="EF-hand domain; pfam13405"
                     /db_xref="CDD:463869"
     CDS             1..685
                     /gene="LOC117606539"
                     /coded_by="XM_034329094.2:224..2281"
                     /db_xref="GeneID:117606539"
ORIGIN      
        1 maeyyihsfa qlstiahvci tlalffandt ivpefdknkp lvtglewedp ehvnrlspae
       61 yrklclilsq iavivnkrrl ilrpyfqdye liaknagtvt fthfgrilkf lnivlaseef
      121 sllikrfakd aytvnyvafl qavdevqsyf dkhgmlslsg elldqfpgrv itaelpklpr
      181 peigklmpsk vfgkqtvfhp vmeepradmp lmkiiqqiqr hilenriris effkrfdhln
      241 vgrvtvsqfk rgldglqvss lgrlylaete idalveiykd pndpdrvcwr tfqddidqvf
      301 tvkeleklpn lriesppkei adlkrrgtad wqcvpgsird lceetlqkvr hrinerrili
      361 kqffkdydrn nkghvsraqf rqvlatavvl lseeeeyale qryndelgfn yvlflkelea
      421 ikieeplyks mleekmkina ekpppegsvd eknivlilak ikakivrerv kvdcrarsff
      481 fkrfnlillf yakaiefmrp ydprneqvik kvdfmrgldq lrcnlsctei gtimkvfqsp
      541 frenyvdyvr fgevveeavt vgslerapll vpvqhvppea spktfltfde rhmatvamdk
      601 lsrmqrpnll elfkdydken igtvskncli kalsvrhmfq lisnreldav ykcfsvekgg
      661 qleinyrafl ealhlmrenr kylpf
//
LOCUS       XP_034184987             107 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606540 [Osmia lignaria lignaria].
ACCESSION   XP_034184987
VERSION     XP_034184987.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329096.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..107
                     /product="uncharacterized protein LOC117606540"
                     /calculated_mol_wt=11654
     Region          56..92
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..107
                     /gene="LOC117606540"
                     /coded_by="XM_034329096.2:166..489"
                     /db_xref="GeneID:117606540"
ORIGIN      
        1 mksifilcct yliissviag vpltrledsq snlskncqip kcpeashved qgktvnlpyp
       61 ldclqyveck gseasiqpcp pdeifdqnts scgprdkvrc vpcyqqg
//
LOCUS       XP_076545648             619 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545648
VERSION     XP_076545648.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689533.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..619
                     /product="liprin-beta 1 isoform X1"
                     /calculated_mol_wt=69478
     Region          130..>216
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          309..371
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          384..446
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          469..540
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..619
                     /gene="Liprin-beta"
                     /coded_by="XM_076689533.1:16..1875"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mrtreaqrtk tclfavmdsg elrytymyiy qkstmgksee knesticpge stfltkpssk
       61 askmleaall qmddiisgac aesnndgnme wkdpvkeatk nlvtaikssp rppsppditt
      121 ieillqwmqp erlrklendr ealhmevsvl seqvdaqcnk iqelesmlre kkdslrrmee
      181 alqkeilars aletqklell sslsemklrq aslehetlrs tntvsngerf grhtsqyssl
      241 prppssskkg vvfgkipslv ygahttvpla vrgasarcls aptlaeeeka iiiensnsrv
      301 elitqkplad lsmeeigtwl aniglecyas elrrwgatgs klfdcsqqql ekeieiknvf
      361 hkkkllyaie seksdsdkfl gadkmdsaav lrwlddiglp qhkeafhnak vdgrmlhrlt
      421 tedllnlgvt aqlhaaslrr giqilrelnf efdnlerrsi ngngmdgnnv rlwtnhrvme
      481 wlrmvdlaey apnmrgsgvh gglmvyegrf tsellatlls ispgktllrr hltthfnqil
      541 greivqrkre iestlgfvpl tltarlkvpk ksqftlkrkk skneadygnl vcpletsspd
      601 tpstptstpg spnlnsptf
//
LOCUS       XP_034185439             588 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185439
VERSION     XP_034185439.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329548.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185439.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="liprin-beta 1 isoform X2"
                     /calculated_mol_wt=66388
     Region          <131..>418
                     /region_name="COG4913"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal ATPase domain [Function unknown]"
                     /db_xref="CDD:443941"
     Region          278..340
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          353..415
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          438..509
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..588
                     /gene="Liprin-beta"
                     /coded_by="XM_034329548.2:16..1782"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mrtreaqrtk tclfavmdsg elrytymyiy qkstmgksee knesticpge stfltkpssk
       61 askmleaall qmddiisgac aesnndgnme wkdpvkeatk nlvtaikssp rppsppditt
      121 ieillqwmqp erlrklendr ealhmevsvl seqvdaqcnk iqelesmlre kkdslrrmee
      181 alqkeilars aletqklell sslsemklrq aslehetlrs tntvsngerf grhtsqyssl
      241 prppssskkg vvfaeeekai iiensnsrve litqkpladl smeeigtwla niglecyase
      301 lrrwgatgsk lfdcsqqqle keieiknvfh kkkllyaies eksdsdkflg adkmdsaavl
      361 rwlddiglpq hkeafhnakv dgrmlhrltt edllnlgvta qlhaaslrrg iqilrelnfe
      421 fdnlerrsin gngmdgnnvr lwtnhrvmew lrmvdlaeya pnmrgsgvhg glmvyegrft
      481 sellatllsi spgktllrrh ltthfnqilg reivqrkrei estlgfvplt ltarlkvpkk
      541 sqftlkrkks kneadygnlv cpletsspdt pstptstpgs pnlnsptf
//
LOCUS       XP_034185432             585 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185432
VERSION     XP_034185432.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329541.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185432.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="liprin-beta 1 isoform X3"
                     /calculated_mol_wt=65353
     Region          96..>182
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          275..337
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          350..412
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          435..506
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..585
                     /gene="Liprin-beta"
                     /coded_by="XM_034329541.2:194..1951"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mgkseeknes ticpgestfl tkpsskaskm leaallqmdd iisgacaesn ndgnmewkdp
       61 vkeatknlvt aikssprpps ppdittieil lqwmqperlr klendrealh mevsvlseqv
      121 daqcnkiqel esmlrekkds lrrmeealqk eilarsalet qklellssls emklrqasle
      181 hetlrstntv sngerfgrht sqysslprpp ssskkgvvfg kipslvygah ttvplavrga
      241 sarclsaptl aeeekaiiie nsnsrvelit qkpladlsme eigtwlanig lecyaselrr
      301 wgatgsklfd csqqqlekei eiknvfhkkk llyaieseks dsdkflgadk mdsaavlrwl
      361 ddiglpqhke afhnakvdgr mlhrlttedl lnlgvtaqlh aaslrrgiqi lrelnfefdn
      421 lerrsingng mdgnnvrlwt nhrvmewlrm vdlaeyapnm rgsgvhgglm vyegrftsel
      481 latllsispg ktllrrhltt hfnqilgrei vqrkreiest lgfvpltlta rlkvpkksqf
      541 tlkrkkskne adygnlvcpl etsspdtpst ptstpgspnl nsptf
//
LOCUS       XP_034185433             585 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185433
VERSION     XP_034185433.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329542.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185433.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="liprin-beta 1 isoform X3"
                     /calculated_mol_wt=65353
     Region          96..>182
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          275..337
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          350..412
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          435..506
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..585
                     /gene="Liprin-beta"
                     /coded_by="XM_034329542.2:157..1914"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mgkseeknes ticpgestfl tkpsskaskm leaallqmdd iisgacaesn ndgnmewkdp
       61 vkeatknlvt aikssprpps ppdittieil lqwmqperlr klendrealh mevsvlseqv
      121 daqcnkiqel esmlrekkds lrrmeealqk eilarsalet qklellssls emklrqasle
      181 hetlrstntv sngerfgrht sqysslprpp ssskkgvvfg kipslvygah ttvplavrga
      241 sarclsaptl aeeekaiiie nsnsrvelit qkpladlsme eigtwlanig lecyaselrr
      301 wgatgsklfd csqqqlekei eiknvfhkkk llyaieseks dsdkflgadk mdsaavlrwl
      361 ddiglpqhke afhnakvdgr mlhrlttedl lnlgvtaqlh aaslrrgiqi lrelnfefdn
      421 lerrsingng mdgnnvrlwt nhrvmewlrm vdlaeyapnm rgsgvhgglm vyegrftsel
      481 latllsispg ktllrrhltt hfnqilgrei vqrkreiest lgfvpltlta rlkvpkksqf
      541 tlkrkkskne adygnlvcpl etsspdtpst ptstpgspnl nsptf
//
LOCUS       XP_034185434             585 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185434
VERSION     XP_034185434.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329543.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185434.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="liprin-beta 1 isoform X3"
                     /calculated_mol_wt=65353
     Region          96..>182
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          275..337
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          350..412
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          435..506
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..585
                     /gene="Liprin-beta"
                     /coded_by="XM_034329543.2:107..1864"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mgkseeknes ticpgestfl tkpsskaskm leaallqmdd iisgacaesn ndgnmewkdp
       61 vkeatknlvt aikssprpps ppdittieil lqwmqperlr klendrealh mevsvlseqv
      121 daqcnkiqel esmlrekkds lrrmeealqk eilarsalet qklellssls emklrqasle
      181 hetlrstntv sngerfgrht sqysslprpp ssskkgvvfg kipslvygah ttvplavrga
      241 sarclsaptl aeeekaiiie nsnsrvelit qkpladlsme eigtwlanig lecyaselrr
      301 wgatgsklfd csqqqlekei eiknvfhkkk llyaieseks dsdkflgadk mdsaavlrwl
      361 ddiglpqhke afhnakvdgr mlhrlttedl lnlgvtaqlh aaslrrgiqi lrelnfefdn
      421 lerrsingng mdgnnvrlwt nhrvmewlrm vdlaeyapnm rgsgvhgglm vyegrftsel
      481 latllsispg ktllrrhltt hfnqilgrei vqrkreiest lgfvpltlta rlkvpkksqf
      541 tlkrkkskne adygnlvcpl etsspdtpst ptstpgspnl nsptf
//
LOCUS       XP_034185435             575 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185435
VERSION     XP_034185435.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329544.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185435.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="liprin-beta 1 isoform X4"
                     /calculated_mol_wt=64302
     Region          86..>172
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          265..327
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          340..402
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          425..496
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..575
                     /gene="Liprin-beta"
                     /coded_by="XM_034329544.2:172..1899"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mvprgestfl tkpsskaskm leaallqmdd iisgacaesn ndgnmewkdp vkeatknlvt
       61 aikssprpps ppdittieil lqwmqperlr klendrealh mevsvlseqv daqcnkiqel
      121 esmlrekkds lrrmeealqk eilarsalet qklellssls emklrqasle hetlrstntv
      181 sngerfgrht sqysslprpp ssskkgvvfg kipslvygah ttvplavrga sarclsaptl
      241 aeeekaiiie nsnsrvelit qkpladlsme eigtwlanig lecyaselrr wgatgsklfd
      301 csqqqlekei eiknvfhkkk llyaieseks dsdkflgadk mdsaavlrwl ddiglpqhke
      361 afhnakvdgr mlhrlttedl lnlgvtaqlh aaslrrgiqi lrelnfefdn lerrsingng
      421 mdgnnvrlwt nhrvmewlrm vdlaeyapnm rgsgvhgglm vyegrftsel latllsispg
      481 ktllrrhltt hfnqilgrei vqrkreiest lgfvpltlta rlkvpkksqf tlkrkkskne
      541 adygnlvcpl etsspdtpst ptstpgspnl nsptf
//
LOCUS       XP_034185437             556 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034185437
VERSION     XP_034185437.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329546.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185437.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..556
                     /product="liprin-beta 1 isoform X5"
                     /calculated_mol_wt=62268
     Region          67..>153
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          246..308
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          321..383
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          406..477
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..556
                     /gene="Liprin-beta"
                     /coded_by="XM_034329546.2:184..1854"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mleaallqmd diisgacaes nndgnmewkd pvkeatknlv taikssprpp sppdittiei
       61 llqwmqperl rklendreal hmevsvlseq vdaqcnkiqe lesmlrekkd slrrmeealq
      121 keilarsale tqklellssl semklrqasl ehetlrstnt vsngerfgrh tsqysslprp
      181 pssskkgvvf gkipslvyga httvplavrg asarclsapt laeeekaiii ensnsrveli
      241 tqkpladlsm eeigtwlani glecyaselr rwgatgsklf dcsqqqleke ieiknvfhkk
      301 kllyaiesek sdsdkflgad kmdsaavlrw lddiglpqhk eafhnakvdg rmlhrltted
      361 llnlgvtaql haaslrrgiq ilrelnfefd nlerrsingn gmdgnnvrlw tnhrvmewlr
      421 mvdlaeyapn mrgsgvhggl mvyegrftse llatllsisp gktllrrhlt thfnqilgre
      481 ivqrkreies tlgfvpltlt arlkvpkksq ftlkrkkskn eadygnlvcp letsspdtps
      541 tptstpgspn lnsptf
//
LOCUS       XP_034185438             556 aa            linear   INV 03-SEP-2025
DEFINITION  liprin-beta 1 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034185438
VERSION     XP_034185438.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329547.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185438.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..556
                     /product="liprin-beta 1 isoform X5"
                     /calculated_mol_wt=62268
     Region          67..>153
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          246..308
                     /region_name="SAM_liprin-beta1,2_repeat1"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 1;
                     cd09563"
                     /db_xref="CDD:188962"
     Region          321..383
                     /region_name="SAM_liprin-beta1,2_repeat2"
                     /note="SAM domain of liprin-beta1,2 proteins repeat 2;
                     cd09566"
                     /db_xref="CDD:188965"
     Region          406..477
                     /region_name="SAM_liprin-beta1,2_repeat3"
                     /note="SAM domain of liprin-beta proteins repeat 3;
                     cd09569"
                     /db_xref="CDD:188968"
     CDS             1..556
                     /gene="Liprin-beta"
                     /coded_by="XM_034329547.2:165..1835"
                     /db_xref="GeneID:117606723"
ORIGIN      
        1 mleaallqmd diisgacaes nndgnmewkd pvkeatknlv taikssprpp sppdittiei
       61 llqwmqperl rklendreal hmevsvlseq vdaqcnkiqe lesmlrekkd slrrmeealq
      121 keilarsale tqklellssl semklrqasl ehetlrstnt vsngerfgrh tsqysslprp
      181 pssskkgvvf gkipslvyga httvplavrg asarclsapt laeeekaiii ensnsrveli
      241 tqkpladlsm eeigtwlani glecyaselr rwgatgsklf dcsqqqleke ieiknvfhkk
      301 kllyaiesek sdsdkflgad kmdsaavlrw lddiglpqhk eafhnakvdg rmlhrltted
      361 llnlgvtaql haaslrrgiq ilrelnfefd nlerrsingn gmdgnnvrlw tnhrvmewlr
      421 mvdlaeyapn mrgsgvhggl mvyegrftse llatllsisp gktllrrhlt thfnqilgre
      481 ivqrkreies tlgfvpltlt arlkvpkksq ftlkrkkskn eadygnlvcp letsspdtps
      541 tptstpgspn lnsptf
//
LOCUS       XP_034190513             255 aa            linear   INV 03-SEP-2025
DEFINITION  DUF1676 domain-containing protein Osi23 [Osmia lignaria lignaria].
ACCESSION   XP_034190513
VERSION     XP_034190513.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334622.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="DUF1676 domain-containing protein Osi23"
                     /calculated_mol_wt=28993
     Region          58..215
                     /region_name="DUF1676"
                     /note="Protein of unknown function (DUF1676); pfam07898"
                     /db_xref="CDD:462310"
     CDS             1..255
                     /gene="Osi23"
                     /coded_by="XM_034334622.2:477..1244"
                     /db_xref="GeneID:117608893"
ORIGIN      
        1 mhprafytla lmllvagdvg cddktkwyvg lqsvfkncpl eswrnagqde fmeklkrciq
       61 ehvlavldsi lvndvipvfd glylvrfqqd rtnstdnytn seleddtnwt amilkrligv
      121 lrthvfkvdv dhlvdstlsp vnnsetelng nifegrrrrf rrkynnvlpf mmmgfllmgs
      181 ilvpmgfqfl avlggkalll akmalilssi qglkkiatng vnyglyhtqv dgwhdrshie
      241 tpshldlpfq phirp
//
LOCUS       XP_034190514             255 aa            linear   INV 03-SEP-2025
DEFINITION  DUF1676 domain-containing protein Osi23 [Osmia lignaria lignaria].
ACCESSION   XP_034190514
VERSION     XP_034190514.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334623.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="DUF1676 domain-containing protein Osi23"
                     /calculated_mol_wt=28993
     Region          58..215
                     /region_name="DUF1676"
                     /note="Protein of unknown function (DUF1676); pfam07898"
                     /db_xref="CDD:462310"
     CDS             1..255
                     /gene="Osi23"
                     /coded_by="XM_034334623.2:1589..2356"
                     /db_xref="GeneID:117608893"
ORIGIN      
        1 mhprafytla lmllvagdvg cddktkwyvg lqsvfkncpl eswrnagqde fmeklkrciq
       61 ehvlavldsi lvndvipvfd glylvrfqqd rtnstdnytn seleddtnwt amilkrligv
      121 lrthvfkvdv dhlvdstlsp vnnsetelng nifegrrrrf rrkynnvlpf mmmgfllmgs
      181 ilvpmgfqfl avlggkalll akmalilssi qglkkiatng vnyglyhtqv dgwhdrshie
      241 tpshldlpfq phirp
//
LOCUS       XP_034190299             365 aa            linear   INV 03-SEP-2025
DEFINITION  twist [Osmia lignaria lignaria].
ACCESSION   XP_034190299
VERSION     XP_034190299.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334408.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="twist"
                     /calculated_mol_wt=41782
     Region          243..310
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(261..262,264,269,288..289)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381418"
     Site            order(267..268,271..272,274..275,279,289..290,293..294,
                     297,299..300,303..304)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381418"
     CDS             1..365
                     /gene="Twi"
                     /coded_by="XM_034334408.2:231..1328"
                     /db_xref="GeneID:117608796"
ORIGIN      
        1 mqatqpvslq aqriqglyll dshdssgiph stgssasnsp dhyerfsppt hlmdlssppe
       61 hrdlpgyqth hhhhhhhqvl yqqptylmye npedekryqd hpngkvlrdl qtdydrrihd
      121 nspgflsdhs rdheqnlylt pspqmyssgg eeivsrqsqq gyhhvdsvey kpdvleykpd
      181 vdqqrykqle msqisepsss tksytvegsr nggkrkrkts sienesetes nasstkskvr
      241 rksgatfeev qnqrvmanvr erqrtqslne afaalrkiip tlpsdklski qtlklatryi
      301 dflfqvlhcn mentdsgdds gernsrsavl aareitsssc symaheklsy afsvwrmedd
      361 wnsni
//
LOCUS       XP_034185460             524 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear RNA export factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185460
VERSION     XP_034185460.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329569.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185460.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..524
                     /product="nuclear RNA export factor 1"
                     /calculated_mol_wt=60170
     Region          144..185
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275382"
     Region          186..209
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275382"
     Region          <187..>244
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          210..233
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275382"
     Region          234..264
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275382"
     Region          474..522
                     /region_name="TAP_C"
                     /note="TAP C-terminal domain; pfam03943"
                     /db_xref="CDD:461102"
     CDS             1..524
                     /gene="LOC117606732"
                     /coded_by="XM_034329569.2:395..1969"
                     /db_xref="GeneID:117606732"
ORIGIN      
        1 mqrtnlpivp mqldssiaik ismggsmfqe rtlmgrsdvw hkvkivkgtq yskesvlkai
       61 lsaiepadli pvkyqasged tyflarncah aldklcktnl iiknpegdpl ilivtlgyas
      121 ihdlklnlqp lllaamskry dsnkkslnle qfhkdpemfk tiycplsqpr tfnhvmklmk
      181 taigtvehln lqkneltnls aiensnvtti knldlrynri ismdvlaalk dlritklwld
      241 gnplcenysn skqyiqsvkr ycphlilldg vyirstdlpl myvnyfktes reelvtrfvn
      301 hffnlydhgd rtvlrglyyk nafysmsfgi tttvaqkrnl gqftanrnll rkvdpnkkrq
      361 hlyygqdnil gglkrlprsy hdrnsfgcdl myddgkclai svsglfkvli nspqvlsfnr
      421 tfvlfagpdn eynilndqyh vdstseeisp nsieskisfe eitplcfsts ekkelfnkfm
      481 evttlnhewc htyleeakwn irkaisnfmk dykssaipsd aflr
//
LOCUS       XP_034184988             352 aa            linear   INV 03-SEP-2025
DEFINITION  histone-lysine N-methyltransferase SETMAR-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034184988
VERSION     XP_034184988.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329097.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="histone-lysine N-methyltransferase SETMAR-like"
                     /calculated_mol_wt=40677
     Region          5..53
                     /region_name="HTH_48"
                     /note="HTH domain in Mos1 transposase; pfam17906"
                     /db_xref="CDD:465558"
     Region          39..315
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..352
                     /gene="LOC117606541"
                     /coded_by="XM_034329097.2:450..1508"
                     /db_xref="GeneID:117606541"
ORIGIN      
        1 mdlnrehfra mifydfkvgl srvkslerlr gafgdsspsq atvyrwfref qrgrgslvde
       61 ervgrpvtaa tdenvlaveq mlsednrmty aqiqgalkig saavktilhe klsvrklssr
      121 wiphqladaq kqdrvkwcqe mlqrfnkgrs eavyniitgd eswiyqyese nkqqsavwvf
      181 sgetppmkmk rgqsvgkkml ctffsasghi etvtledqiv taewytavcl pqvfekletk
      241 rpkidmkgil lhhdsalvhs aektanyfre lgiqvlghpp yspdlapcdf flfpkakesi
      301 rgqrfeslet aiatykefld sipkeewrae fgkwfdrmrt cirregeyfe ki
//
LOCUS       XP_076545649             352 aa            linear   INV 03-SEP-2025
DEFINITION  histone-lysine N-methyltransferase SETMAR-like [Osmia lignaria
            lignaria].
ACCESSION   XP_076545649
VERSION     XP_076545649.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689534.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="histone-lysine N-methyltransferase SETMAR-like"
                     /calculated_mol_wt=40677
     Region          5..53
                     /region_name="HTH_48"
                     /note="HTH domain in Mos1 transposase; pfam17906"
                     /db_xref="CDD:465558"
     Region          39..315
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     CDS             1..352
                     /gene="LOC117606541"
                     /coded_by="XM_076689534.1:323..1381"
                     /db_xref="GeneID:117606541"
ORIGIN      
        1 mdlnrehfra mifydfkvgl srvkslerlr gafgdsspsq atvyrwfref qrgrgslvde
       61 ervgrpvtaa tdenvlaveq mlsednrmty aqiqgalkig saavktilhe klsvrklssr
      121 wiphqladaq kqdrvkwcqe mlqrfnkgrs eavyniitgd eswiyqyese nkqqsavwvf
      181 sgetppmkmk rgqsvgkkml ctffsasghi etvtledqiv taewytavcl pqvfekletk
      241 rpkidmkgil lhhdsalvhs aektanyfre lgiqvlghpp yspdlapcdf flfpkakesi
      301 rgqrfeslet aiatykefld sipkeewrae fgkwfdrmrt cirregeyfe ki
//
LOCUS       XP_034185020             403 aa            linear   INV 03-SEP-2025
DEFINITION  lipase 3 [Osmia lignaria lignaria].
ACCESSION   XP_034185020
VERSION     XP_034185020.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329129.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185020.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="lipase 3"
                     /calculated_mol_wt=45609
     Region          41..399
                     /region_name="PLN02872"
                     /note="triacylglycerol lipase"
                     /db_xref="CDD:215470"
     CDS             1..403
                     /gene="LOC117606561"
                     /coded_by="XM_034329129.2:40..1251"
                     /db_xref="GeneID:117606561"
ORIGIN      
        1 mlkifyvscl vlpfvlgvpi keeenvpssn ldtkfifpdp aelarrkgyn aethqvtted
       61 gyilqmhrvt grqntskpkp avlllhglfd caatwvisdp trslafllsd rgydvwlgnv
      121 rgsrysrkhr vmnttngkfw kfswheigty dvpamidyii ektkqekiyy ighsqggtsf
      181 fvmaserpey qkkiiasfnl apavfmgnsr naflrllapf asdlkalgdl iglyefmpsq
      241 tllkilatkg cqenaplmpm cknimflaag vdkelnttlv pqvvrydpag asvmqlvhyg
      301 qlmnsgkfqr fdhglitnlq tykqiqppqy nlnnitvpvy ilystndalv dykdsirlse
      361 vvpsaeklli pnkdyahldf vwgnnvdslv ynkifsllkq yks
//
LOCUS       XP_034194852             459 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611076 [Osmia lignaria lignaria].
ACCESSION   XP_034194852
VERSION     XP_034194852.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338961.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194852.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="uncharacterized protein LOC117611076"
                     /calculated_mol_wt=53268
     Region          206..396
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(262,296,306,308)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..459
                     /gene="LOC117611076"
                     /coded_by="XM_034338961.2:1705..3084"
                     /db_xref="GeneID:117611076"
ORIGIN      
        1 mdilsisshi fgehkeynsr shtcmrdvnr aekdyvpslk lyglyievek airflscysd
       61 slltnvtnnp aetfnsiick eiggkrinfg argsynarva gavtqyntqq eltemhknmc
      121 ktipplvkrl eerrqrkvak tkelrmthgk rrkfkpvqgt dryygpnsqr pdlpsdillq
      181 lwkehfekla dnarnrtiie tntreqndsd lwralkekml tasnfgavcl mrqttscaam
      241 aknilypppv dtaamkygry kehiarkdva namktrirtc glfidrdipy lgaspdglid
      301 ddgllelkcp qsaenltain aietirhlrn ifdkrdiqem nrkhqyfyqv qgqlhitqrk
      361 ycifaiwtpk sihmirvnrd dafwtnhmep fvtrfyegcm lleildsrhn rhmpirnpey
      421 ilrakedtas iqkcsrqiae krtyenmtqn svvasnitk
//
LOCUS       XP_034194854             459 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611076 [Osmia lignaria lignaria].
ACCESSION   XP_034194854
VERSION     XP_034194854.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338963.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194854.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="uncharacterized protein LOC117611076"
                     /calculated_mol_wt=53268
     Region          206..396
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(262,296,306,308)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..459
                     /gene="LOC117611076"
                     /coded_by="XM_034338963.2:1705..3084"
                     /db_xref="GeneID:117611076"
ORIGIN      
        1 mdilsisshi fgehkeynsr shtcmrdvnr aekdyvpslk lyglyievek airflscysd
       61 slltnvtnnp aetfnsiick eiggkrinfg argsynarva gavtqyntqq eltemhknmc
      121 ktipplvkrl eerrqrkvak tkelrmthgk rrkfkpvqgt dryygpnsqr pdlpsdillq
      181 lwkehfekla dnarnrtiie tntreqndsd lwralkekml tasnfgavcl mrqttscaam
      241 aknilypppv dtaamkygry kehiarkdva namktrirtc glfidrdipy lgaspdglid
      301 ddgllelkcp qsaenltain aietirhlrn ifdkrdiqem nrkhqyfyqv qgqlhitqrk
      361 ycifaiwtpk sihmirvnrd dafwtnhmep fvtrfyegcm lleildsrhn rhmpirnpey
      421 ilrakedtas iqkcsrqiae krtyenmtqn svvasnitk
//
LOCUS       XP_034194855             459 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611076 [Osmia lignaria lignaria].
ACCESSION   XP_034194855
VERSION     XP_034194855.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338964.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194855.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="uncharacterized protein LOC117611076"
                     /calculated_mol_wt=53268
     Region          206..396
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(262,296,306,308)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..459
                     /gene="LOC117611076"
                     /coded_by="XM_034338964.2:1705..3084"
                     /db_xref="GeneID:117611076"
ORIGIN      
        1 mdilsisshi fgehkeynsr shtcmrdvnr aekdyvpslk lyglyievek airflscysd
       61 slltnvtnnp aetfnsiick eiggkrinfg argsynarva gavtqyntqq eltemhknmc
      121 ktipplvkrl eerrqrkvak tkelrmthgk rrkfkpvqgt dryygpnsqr pdlpsdillq
      181 lwkehfekla dnarnrtiie tntreqndsd lwralkekml tasnfgavcl mrqttscaam
      241 aknilypppv dtaamkygry kehiarkdva namktrirtc glfidrdipy lgaspdglid
      301 ddgllelkcp qsaenltain aietirhlrn ifdkrdiqem nrkhqyfyqv qgqlhitqrk
      361 ycifaiwtpk sihmirvnrd dafwtnhmep fvtrfyegcm lleildsrhn rhmpirnpey
      421 ilrakedtas iqkcsrqiae krtyenmtqn svvasnitk
//
LOCUS       XP_034194856             459 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611076 [Osmia lignaria lignaria].
ACCESSION   XP_034194856
VERSION     XP_034194856.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338965.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194856.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="uncharacterized protein LOC117611076"
                     /calculated_mol_wt=53268
     Region          206..396
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(262,296,306,308)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..459
                     /gene="LOC117611076"
                     /coded_by="XM_034338965.2:1705..3084"
                     /db_xref="GeneID:117611076"
ORIGIN      
        1 mdilsisshi fgehkeynsr shtcmrdvnr aekdyvpslk lyglyievek airflscysd
       61 slltnvtnnp aetfnsiick eiggkrinfg argsynarva gavtqyntqq eltemhknmc
      121 ktipplvkrl eerrqrkvak tkelrmthgk rrkfkpvqgt dryygpnsqr pdlpsdillq
      181 lwkehfekla dnarnrtiie tntreqndsd lwralkekml tasnfgavcl mrqttscaam
      241 aknilypppv dtaamkygry kehiarkdva namktrirtc glfidrdipy lgaspdglid
      301 ddgllelkcp qsaenltain aietirhlrn ifdkrdiqem nrkhqyfyqv qgqlhitqrk
      361 ycifaiwtpk sihmirvnrd dafwtnhmep fvtrfyegcm lleildsrhn rhmpirnpey
      421 ilrakedtas iqkcsrqiae krtyenmtqn svvasnitk
//
LOCUS       XP_034185030             987 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606568 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185030
VERSION     XP_034185030.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329139.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185030.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..987
                     /product="uncharacterized protein LOC117606568 isoform X1"
                     /calculated_mol_wt=112185
     CDS             1..987
                     /gene="LOC117606568"
                     /coded_by="XM_034329139.2:1..2964"
                     /db_xref="GeneID:117606568"
ORIGIN      
        1 mrrptvstls rrprqwrhqr atalspaahe fsvstghapi awtllfcfla ipsllslqrl
       61 qihfqvtnqq tkidgksgkv gnskkvsnri lpkalvgiyr ngaagkiikk sqtkeplndi
      121 nnarlqgnvy kllnncdsel epitiengtk gvsknplfds avknilhptp pgdkrlspvl
      181 gkvdgkkqrp kircienvnp prvfnfllkp knknsvniek fpdsaikkye elcstvsaen
      241 fegknlpqfv tdpliatkkk lrsmyaesfr sqylgdtikr gtvlnevias sskdnpsdve
      301 ycslgyvqds dlgsvavekp drqlrhrkdk reslpeseni fvipekhfdl ngrkneappn
      361 llfdlsllnk sssgvenvep eslnemnnlk snltditinn rdfyegnyck rdsnfkaaas
      421 mhvnkmerlk ekmesplfip keaqlpndli tkkhlkrkiq rvqtqfvkhg akkhpgrgnl
      481 kvshqvknlk hipailrrcn naannnnrln lfdpsmhink kpqeilldva pkqsalfspq
      541 tldtesnrns kklettamfd padvqtcvma spsllrdevc fksfreqpvd akqqpqklkt
      601 neiflgkknm npsvqktimf tkkdsrnigh clylnnlitd rsadnsnvcv mrrsvsvdqe
      661 ksmknpcptr rncqqchrcs egsdgsyycs qesnpsqmqn aevhqkpvvc ekvllrrvqq
      721 hlhsnqkchn dqhvcyvvvd pnsvqecssd nvscqkekhr tvhprhfyqd dvsdigilkg
      781 vqnlqilpme erelqtnfts nqctgrnavv ieeqpikyla vennsraqki piymqsnkrv
      841 tsvdkvevvn pnvgyrvvsc pkessqkvif vptceqskav ygkqenlasy desplarkvi
      901 lcrpefqsnl wyvkdpsnvc evhvpkqnlm evrntkcdtv vnnlqgdkvk lsrrstvnwd
      961 dlhyrsnhey qhvndgcvrn htifykk
//
LOCUS       XP_034185033             971 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606568 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185033
VERSION     XP_034185033.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185033.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..971
                     /product="uncharacterized protein LOC117606568 isoform X2"
                     /calculated_mol_wt=110604
     CDS             1..971
                     /gene="LOC117606568"
                     /coded_by="XM_034329142.2:562..3477"
                     /db_xref="GeneID:117606568"
ORIGIN      
        1 mgkvirdkaa sdsseeeqrk ptgeerkvrr kkkkckkdlk elqrrllksk rnqqtkidgk
       61 sgkvgnskkv snrilpkalv giyrngaagk iikksqtkep lndinnarlq gnvykllnnc
      121 dselepitie ngtkgvsknp lfdsavknil hptppgdkrl spvlgkvdgk kqrpkircie
      181 nvnpprvfnf llkpknknsv niekfpdsai kkyeelcstv saenfegknl pqfvtdplia
      241 tkkklrsmya esfrsqylgd tikrgtvlne viassskdnp sdveycslgy vqdsdlgsva
      301 vekpdrqlrh rkdkreslpe senifvipek hfdlngrkne appnllfdls llnksssgve
      361 nvepeslnem nnlksnltdi tinnrdfyeg nyckrdsnfk aaasmhvnkm erlkekmesp
      421 lfipkeaqlp ndlitkkhlk rkiqrvqtqf vkhgakkhpg rgnlkvshqv knlkhipail
      481 rrcnnaannn nrlnlfdpsm hinkkpqeil ldvapkqsal fspqtldtes nrnskklett
      541 amfdpadvqt cvmaspsllr devcfksfre qpvdakqqpq klktneiflg kknmnpsvqk
      601 timftkkdsr nighclylnn litdrsadns nvcvmrrsvs vdqeksmknp cptrrncqqc
      661 hrcsegsdgs yycsqesnps qmqnaevhqk pvvcekvllr rvqqhlhsnq kchndqhvcy
      721 vvvdpnsvqe cssdnvscqk ekhrtvhprh fyqddvsdig ilkgvqnlqi lpmeerelqt
      781 nftsnqctgr navvieeqpi kylavennsr aqkipiymqs nkrvtsvdkv evvnpnvgyr
      841 vvscpkessq kvifvptceq skavygkqen lasydespla rkvilcrpef qsnlwyvkdp
      901 snvcevhvpk qnlmevrntk cdtvvnnlqg dkvklsrrst vnwddlhyrs nheyqhvndg
      961 cvrnhtifyk k
//
LOCUS       XP_076545650             963 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606568 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545650
VERSION     XP_076545650.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689535.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..963
                     /product="uncharacterized protein LOC117606568 isoform X3"
                     /calculated_mol_wt=109676
     CDS             1..963
                     /gene="LOC117606568"
                     /coded_by="XM_076689535.1:1..2892"
                     /db_xref="GeneID:117606568"
ORIGIN      
        1 mrrptvstls rrprqwrhqr atalspaahe fsvstghapi awtllfcfla ipsllslqrl
       61 qihfqvtnqq tkidgksgkv gnskkvsnri lpkalvgiyr ngaagkiikk sqtkeplndi
      121 nnarlqgnvy kllnncdsel epitiengtk gvsknplfds avknilhptp pgdkrlspvl
      181 gkvdgkkqrp kircienvnp prvfnfllkp knknsvniek fpdsaikkye elcstvsaen
      241 fegknlpqfv tdpliatkkk lrsmyaesfr sqylgdtikr gyvqdsdlgs vavekpdrql
      301 rhrkdkresl pesenifvip ekhfdlngrk neappnllfd lsllnksssg venvepesln
      361 emnnlksnlt ditinnrdfy egnyckrdsn fkaaasmhvn kmerlkekme splfipkeaq
      421 lpndlitkkh lkrkiqrvqt qfvkhgakkh pgrgnlkvsh qvknlkhipa ilrrcnnaan
      481 nnnrlnlfdp smhinkkpqe illdvapkqs alfspqtldt esnrnskkle ttamfdpadv
      541 qtcvmaspsl lrdevcfksf reqpvdakqq pqklktneif lgkknmnpsv qktimftkkd
      601 srnighclyl nnlitdrsad nsnvcvmrrs vsvdqeksmk npcptrrncq qchrcsegsd
      661 gsyycsqesn psqmqnaevh qkpvvcekvl lrrvqqhlhs nqkchndqhv cyvvvdpnsv
      721 qecssdnvsc qkekhrtvhp rhfyqddvsd igilkgvqnl qilpmeerel qtnftsnqct
      781 grnavvieeq pikylavenn sraqkipiym qsnkrvtsvd kvevvnpnvg yrvvscpkes
      841 sqkvifvptc eqskavygkq enlasydesp larkvilcrp efqsnlwyvk dpsnvcevhv
      901 pkqnlmevrn tkcdtvvnnl qgdkvklsrr stvnwddlhy rsnheyqhvn dgcvrnhtif
      961 ykk
//
LOCUS       XP_034185034             792 aa            linear   INV 03-SEP-2025
DEFINITION  neprilysin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034185034
VERSION     XP_034185034.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185034.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..792
                     /product="neprilysin-1"
                     /calculated_mol_wt=90294
     Region          138..790
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(168,580,585..588,607,623..624,627..628,631,689,732,
                     735..736,753..754,760)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..792
                     /gene="LOC117606569"
                     /coded_by="XM_034329143.2:1313..3691"
                     /db_xref="GeneID:117606569"
ORIGIN      
        1 mkaegnngyl ratviteqne itgtagqetp aantlqvtyg prgsttttvl qhpgnsrrpi
       61 ggitratsit rrrrepasrq qllgvlavtl latclfilll lalntsrecv qeprpnvcmt
      121 eecvrtaasl lsamdrtaap cvnffqyacg twnrlhvipe drssistfev ladqlqvilk
      181 rvleeppndq dnnatlkakm fykscmdipr ireigdvplk rtleylggwp vvvgpswkpp
      241 pypvevllgr lrgeynegvl leqwvgpddk nssanilqld qmqlalpsrd yylkknseae
      301 lkayhrymts vavllganpk taaeefdevi kfekklayas lpeadrhdts aiyrkltlre
      361 lqreipqlqw raylqefisa pitedepiva yampyfmqmg rivrsthrrt lhnyilwrlv
      421 msimphmide yqqkrvefrk illgilsegn rwsqcvdwtn kklgmavgal firdnfnhds
      481 ketalemirt ireafnella enhwmddetr avakskadsm nerigypefl kdpvelskey
      541 vvlnitenhf lenvlavlky dayhnlqklr kpvdknkwst epavvnafyn pnkndivfpa
      601 gilqplfysq hfpkslnygg igvvigheit hgfddkgrqf dkdgnmmqww ndatirafrq
      661 raqcivdqys ryklqevdly ingrmtqgen iadngglkqs fraykkwvsi hgeepllpgv
      721 nlthdqlffl nyaqiwcgsm rpedaltkir ssvhspgpir vlgplsnsed faraydcppg
      781 spmnpihkcs vw
//
LOCUS       XP_034185035             792 aa            linear   INV 03-SEP-2025
DEFINITION  neprilysin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034185035
VERSION     XP_034185035.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329144.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185035.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..792
                     /product="neprilysin-1"
                     /calculated_mol_wt=90294
     Region          138..790
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(168,580,585..588,607,623..624,627..628,631,689,732,
                     735..736,753..754,760)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..792
                     /gene="LOC117606569"
                     /coded_by="XM_034329144.2:1334..3712"
                     /db_xref="GeneID:117606569"
ORIGIN      
        1 mkaegnngyl ratviteqne itgtagqetp aantlqvtyg prgsttttvl qhpgnsrrpi
       61 ggitratsit rrrrepasrq qllgvlavtl latclfilll lalntsrecv qeprpnvcmt
      121 eecvrtaasl lsamdrtaap cvnffqyacg twnrlhvipe drssistfev ladqlqvilk
      181 rvleeppndq dnnatlkakm fykscmdipr ireigdvplk rtleylggwp vvvgpswkpp
      241 pypvevllgr lrgeynegvl leqwvgpddk nssanilqld qmqlalpsrd yylkknseae
      301 lkayhrymts vavllganpk taaeefdevi kfekklayas lpeadrhdts aiyrkltlre
      361 lqreipqlqw raylqefisa pitedepiva yampyfmqmg rivrsthrrt lhnyilwrlv
      421 msimphmide yqqkrvefrk illgilsegn rwsqcvdwtn kklgmavgal firdnfnhds
      481 ketalemirt ireafnella enhwmddetr avakskadsm nerigypefl kdpvelskey
      541 vvlnitenhf lenvlavlky dayhnlqklr kpvdknkwst epavvnafyn pnkndivfpa
      601 gilqplfysq hfpkslnygg igvvigheit hgfddkgrqf dkdgnmmqww ndatirafrq
      661 raqcivdqys ryklqevdly ingrmtqgen iadngglkqs fraykkwvsi hgeepllpgv
      721 nlthdqlffl nyaqiwcgsm rpedaltkir ssvhspgpir vlgplsnsed faraydcppg
      781 spmnpihkcs vw
//
LOCUS       XP_034185036             792 aa            linear   INV 03-SEP-2025
DEFINITION  neprilysin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034185036
VERSION     XP_034185036.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329145.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185036.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..792
                     /product="neprilysin-1"
                     /calculated_mol_wt=90294
     Region          138..790
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(168,580,585..588,607,623..624,627..628,631,689,732,
                     735..736,753..754,760)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..792
                     /gene="LOC117606569"
                     /coded_by="XM_034329145.2:1306..3684"
                     /db_xref="GeneID:117606569"
ORIGIN      
        1 mkaegnngyl ratviteqne itgtagqetp aantlqvtyg prgsttttvl qhpgnsrrpi
       61 ggitratsit rrrrepasrq qllgvlavtl latclfilll lalntsrecv qeprpnvcmt
      121 eecvrtaasl lsamdrtaap cvnffqyacg twnrlhvipe drssistfev ladqlqvilk
      181 rvleeppndq dnnatlkakm fykscmdipr ireigdvplk rtleylggwp vvvgpswkpp
      241 pypvevllgr lrgeynegvl leqwvgpddk nssanilqld qmqlalpsrd yylkknseae
      301 lkayhrymts vavllganpk taaeefdevi kfekklayas lpeadrhdts aiyrkltlre
      361 lqreipqlqw raylqefisa pitedepiva yampyfmqmg rivrsthrrt lhnyilwrlv
      421 msimphmide yqqkrvefrk illgilsegn rwsqcvdwtn kklgmavgal firdnfnhds
      481 ketalemirt ireafnella enhwmddetr avakskadsm nerigypefl kdpvelskey
      541 vvlnitenhf lenvlavlky dayhnlqklr kpvdknkwst epavvnafyn pnkndivfpa
      601 gilqplfysq hfpkslnygg igvvigheit hgfddkgrqf dkdgnmmqww ndatirafrq
      661 raqcivdqys ryklqevdly ingrmtqgen iadngglkqs fraykkwvsi hgeepllpgv
      721 nlthdqlffl nyaqiwcgsm rpedaltkir ssvhspgpir vlgplsnsed faraydcppg
      781 spmnpihkcs vw
//
LOCUS       XP_034185038             595 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185038
VERSION     XP_034185038.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329147.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="ecdysone receptor isoform X1"
                     /calculated_mol_wt=64588
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          363..593
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(378..380,406..407,409..410,413,447,450..451,454,462,
                     464..465,475,487,572)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(415,418,422,427,432..433,435..436,439..440,585..586,
                     589)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(489,526,529,533,537,553..554,557,559,561,563..564)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..595
                     /gene="Ecr"
                     /coded_by="XM_034329147.2:2634..4421"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkstnv
      301 slqekdkpns ttmngspgsg iraepmgvki epaeaeslst sgssgiltpv spygyvkpis
      361 peqeelihrl vyfqneyeqp seedlkritn qpsegedisd ykfrhiteit iltvqlivef
      421 skrlpgfdel mredqiallk acssevmmlr markydvqtd siifannqpy sresynlagm
      481 getiedllhf crqmyamkvn naeyalltai vifserpnll egwkvekiqe iylealkayv
      541 dnrrrpksgt ifakllsvlt elrtlgnqns eicfslkfkn kklplflaei wdvtp
//
LOCUS       XP_034185039             595 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185039
VERSION     XP_034185039.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329148.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="ecdysone receptor isoform X1"
                     /calculated_mol_wt=64588
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          363..593
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(378..380,406..407,409..410,413,447,450..451,454,462,
                     464..465,475,487,572)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(415,418,422,427,432..433,435..436,439..440,585..586,
                     589)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(489,526,529,533,537,553..554,557,559,561,563..564)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..595
                     /gene="Ecr"
                     /coded_by="XM_034329148.2:2691..4478"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkstnv
      301 slqekdkpns ttmngspgsg iraepmgvki epaeaeslst sgssgiltpv spygyvkpis
      361 peqeelihrl vyfqneyeqp seedlkritn qpsegedisd ykfrhiteit iltvqlivef
      421 skrlpgfdel mredqiallk acssevmmlr markydvqtd siifannqpy sresynlagm
      481 getiedllhf crqmyamkvn naeyalltai vifserpnll egwkvekiqe iylealkayv
      541 dnrrrpksgt ifakllsvlt elrtlgnqns eicfslkfkn kklplflaei wdvtp
//
LOCUS       XP_034185040             595 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185040
VERSION     XP_034185040.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329149.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="ecdysone receptor isoform X1"
                     /calculated_mol_wt=64588
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          363..593
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(378..380,406..407,409..410,413,447,450..451,454,462,
                     464..465,475,487,572)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(415,418,422,427,432..433,435..436,439..440,585..586,
                     589)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(489,526,529,533,537,553..554,557,559,561,563..564)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..595
                     /gene="Ecr"
                     /coded_by="XM_034329149.2:2333..4120"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkstnv
      301 slqekdkpns ttmngspgsg iraepmgvki epaeaeslst sgssgiltpv spygyvkpis
      361 peqeelihrl vyfqneyeqp seedlkritn qpsegedisd ykfrhiteit iltvqlivef
      421 skrlpgfdel mredqiallk acssevmmlr markydvqtd siifannqpy sresynlagm
      481 getiedllhf crqmyamkvn naeyalltai vifserpnll egwkvekiqe iylealkayv
      541 dnrrrpksgt ifakllsvlt elrtlgnqns eicfslkfkn kklplflaei wdvtp
//
LOCUS       XP_034185041             595 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185041
VERSION     XP_034185041.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329150.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="ecdysone receptor isoform X1"
                     /calculated_mol_wt=64588
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          363..593
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(378..380,406..407,409..410,413,447,450..451,454,462,
                     464..465,475,487,572)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(415,418,422,427,432..433,435..436,439..440,585..586,
                     589)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(489,526,529,533,537,553..554,557,559,561,563..564)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..595
                     /gene="Ecr"
                     /coded_by="XM_034329150.2:1257..3044"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkstnv
      301 slqekdkpns ttmngspgsg iraepmgvki epaeaeslst sgssgiltpv spygyvkpis
      361 peqeelihrl vyfqneyeqp seedlkritn qpsegedisd ykfrhiteit iltvqlivef
      421 skrlpgfdel mredqiallk acssevmmlr markydvqtd siifannqpy sresynlagm
      481 getiedllhf crqmyamkvn naeyalltai vifserpnll egwkvekiqe iylealkayv
      541 dnrrrpksgt ifakllsvlt elrtlgnqns eicfslkfkn kklplflaei wdvtp
//
LOCUS       XP_034185042             595 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185042
VERSION     XP_034185042.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329151.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="ecdysone receptor isoform X1"
                     /calculated_mol_wt=64588
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          363..593
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(378..380,406..407,409..410,413,447,450..451,454,462,
                     464..465,475,487,572)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(415,418,422,427,432..433,435..436,439..440,585..586,
                     589)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(489,526,529,533,537,553..554,557,559,561,563..564)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..595
                     /gene="Ecr"
                     /coded_by="XM_034329151.2:842..2629"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkstnv
      301 slqekdkpns ttmngspgsg iraepmgvki epaeaeslst sgssgiltpv spygyvkpis
      361 peqeelihrl vyfqneyeqp seedlkritn qpsegedisd ykfrhiteit iltvqlivef
      421 skrlpgfdel mredqiallk acssevmmlr markydvqtd siifannqpy sresynlagm
      481 getiedllhf crqmyamkvn naeyalltai vifserpnll egwkvekiqe iylealkayv
      541 dnrrrpksgt ifakllsvlt elrtlgnqns eicfslkfkn kklplflaei wdvtp
//
LOCUS       XP_034185043             593 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185043
VERSION     XP_034185043.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329152.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..593
                     /product="ecdysone receptor isoform X2"
                     /calculated_mol_wt=64346
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          363..591
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(378..380,404..405,407..408,411,445,448..449,452,460,
                     462..463,473,485,570)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(413,416,420,425,430..431,433..434,437..438,583..584,
                     587)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(487,524,527,531,535,551..552,555,557,559,561..562)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..593
                     /gene="Ecr"
                     /coded_by="XM_034329152.2:2635..4416"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkstnv
      301 slqekdkpns ttmngspgsg iraepmgvki epaeaeslst sgssgiltpv spygyvkpis
      361 peqeelihrl vyfqneyeqp seedlkritp segedisdyk frhiteitil tvqlivefsk
      421 rlpgfdelmr edqiallkac ssevmmlrma rkydvqtdsi ifannqpysr esynlagmge
      481 tiedllhfcr qmyamkvnna eyalltaivi fserpnlleg wkvekiqeiy lealkayvdn
      541 rrrpksgtif akllsvltel rtlgnqnsei cfslkfknkk lplflaeiwd vtp
//
LOCUS       XP_034185044             588 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185044
VERSION     XP_034185044.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329153.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="ecdysone receptor isoform X3"
                     /calculated_mol_wt=63858
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          356..586
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(371..373,399..400,402..403,406,440,443..444,447,455,
                     457..458,468,480,565)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(408,411,415,420,425..426,428..429,432..433,578..579,
                     582)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(482,519,522,526,530,546..547,550,552,554,556..557)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..588
                     /gene="Ecr"
                     /coded_by="XM_034329153.2:2632..4398"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkekdk
      301 pnsttmngsp gsgiraepmg vkiepaeaes lstsgssgil tpvspygyvk pispeqeeli
      361 hrlvyfqney eqpseedlkr itnqpseged isdykfrhit eitiltvqli vefskrlpgf
      421 delmredqia llkacssevm mlrmarkydv qtdsiifann qpysresynl agmgetiedl
      481 lhfcrqmyam kvnnaeyall taivifserp nllegwkvek iqeiylealk ayvdnrrrpk
      541 sgtifaklls vltelrtlgn qnseicfslk fknkklplfl aeiwdvtp
//
LOCUS       XP_034185045             586 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185045
VERSION     XP_034185045.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329154.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="ecdysone receptor isoform X4"
                     /calculated_mol_wt=63616
     Region          206..296
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(209,212,226,229,245,251,261,264)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(214..215,255..256,259)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(218..221,227..228,230,232,234..235,258..260,262,265,
                     278..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          356..584
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(371..373,397..398,400..401,404,438,441..442,445,453,
                     455..456,466,478,563)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(406,409,413,418,423..424,426..427,430..431,576..577,
                     580)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(480,517,520,524,528,544..545,548,550,552,554..555)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..586
                     /gene="Ecr"
                     /coded_by="XM_034329154.2:2633..4393"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mdtsdssmdt tnggssttva gvpgvgtias vvagaasltl vkaetpehla gtsttpssvt
       61 gpvttaaglf agivsanktp rpddwlstns pgsptaalqs qhvvyttpqq qltdsqqppl
      121 ahssplahqq psnsnngyas pmstssydpy spnskigrde lsqpgslngy gssggggggg
      181 ggggggggsd gcdarkkkgp tprqqeelcl vcgdrasgyh ynaltcegck gffrrsitkn
      241 avyqckygnn ceidmymrrk cqecrlkkcl tvgmrpecvv peyqcavkrq ekkaqkekdk
      301 pnsttmngsp gsgiraepmg vkiepaeaes lstsgssgil tpvspygyvk pispeqeeli
      361 hrlvyfqney eqpseedlkr itpsegedis dykfrhitei tiltvqlive fskrlpgfde
      421 lmredqiall kacssevmml rmarkydvqt dsiifannqp ysresynlag mgetiedllh
      481 fcrqmyamkv nnaeyallta ivifserpnl legwkvekiq eiylealkay vdnrrrpksg
      541 tifakllsvl telrtlgnqn seicfslkfk nkklplflae iwdvtp
//
LOCUS       XP_034185046             544 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034185046
VERSION     XP_034185046.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329155.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..544
                     /product="ecdysone receptor isoform X5"
                     /calculated_mol_wt=60237
     Region          155..245
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(158,161,175,178,194,200,210,213)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(163..164,204..205,208)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(167..170,176..177,179,181,183..184,207..209,211,214,
                     227..230)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          312..542
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(327..329,355..356,358..359,362,396,399..400,403,411,
                     413..414,424,436,521)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(364,367,371,376,381..382,384..385,388..389,534..535,
                     538)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(438,475,478,482,486,502..503,506,508,510,512..513)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..544
                     /gene="Ecr"
                     /coded_by="XM_034329155.2:2283..3917"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mlqtvprvpv agvrrrwgnr htgslqessp evsssgvlsp plnfqpstpe saavrneelq
       61 lwdldlhrgl qsngdhyrat itatttttvl atvadpaspf ttpvegrdel sqpgslngyg
      121 ssgggggggg gggggggsdg cdarkkkgpt prqqeelclv cgdrasgyhy naltcegckg
      181 ffrrsitkna vyqckygnnc eidmymrrkc qecrlkkclt vgmrpecvvp eyqcavkrqe
      241 kkaqkstnvs lqekdkpnst tmngspgsgi raepmgvkie paeaeslsts gssgiltpvs
      301 pygyvkpisp eqeelihrlv yfqneyeqps eedlkritnq psegedisdy kfrhiteiti
      361 ltvqlivefs krlpgfdelm redqiallka cssevmmlrm arkydvqtds iifannqpys
      421 resynlagmg etiedllhfc rqmyamkvnn aeyalltaiv ifserpnlle gwkvekiqei
      481 ylealkayvd nrrrpksgti fakllsvlte lrtlgnqnse icfslkfknk klplflaeiw
      541 dvtp
//
LOCUS       XP_034185048             537 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone receptor isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034185048
VERSION     XP_034185048.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329157.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..537
                     /product="ecdysone receptor isoform X6"
                     /calculated_mol_wt=59507
     Region          155..245
                     /region_name="NR_DBD_EcR"
                     /note="DNA-binding domain of Ecdysone receptor (ECR)
                     family is composed of two C4-type zinc fingers; cd07161"
                     /db_xref="CDD:143535"
     Site            order(158,161,175,178,194,200,210,213)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143535"
     Site            order(163..164,204..205,208)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:143535"
     Site            order(167..170,176..177,179,181,183..184,207..209,211,214,
                     227..230)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143535"
     Region          305..535
                     /region_name="NR_LBD_EcR"
                     /note="The ligand binding domain (LBD) of the Ecdysone
                     receptor, a member of the nuclear receptors super family;
                     cd06938"
                     /db_xref="CDD:132736"
     Site            order(320..322,348..349,351..352,355,389,392..393,396,404,
                     406..407,417,429,514)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132736"
     Site            order(357,360,364,369,374..375,377..378,381..382,527..528,
                     531)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132736"
     Site            order(431,468,471,475,479,495..496,499,501,503,505..506)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132736"
     CDS             1..537
                     /gene="Ecr"
                     /coded_by="XM_034329157.2:2267..3880"
                     /db_xref="GeneID:117606570"
ORIGIN      
        1 mlqtvprvpv agvrrrwgnr htgslqessp evsssgvlsp plnfqpstpe saavrneelq
       61 lwdldlhrgl qsngdhyrat itatttttvl atvadpaspf ttpvegrdel sqpgslngyg
      121 ssgggggggg gggggggsdg cdarkkkgpt prqqeelclv cgdrasgyhy naltcegckg
      181 ffrrsitkna vyqckygnnc eidmymrrkc qecrlkkclt vgmrpecvvp eyqcavkrqe
      241 kkaqkekdkp nsttmngspg sgiraepmgv kiepaeaesl stsgssgilt pvspygyvkp
      301 ispeqeelih rlvyfqneye qpseedlkri tnqpsegedi sdykfrhite itiltvqliv
      361 efskrlpgfd elmredqial lkacssevmm lrmarkydvq tdsiifannq pysresynla
      421 gmgetiedll hfcrqmyamk vnnaeyallt aivifserpn llegwkveki qeiylealka
      481 yvdnrrrpks gtifakllsv ltelrtlgnq nseicfslkf knkklplfla eiwdvtp
//
LOCUS       XP_076545651             461 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305517 [Osmia lignaria lignaria].
ACCESSION   XP_076545651
VERSION     XP_076545651.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689536.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..461
                     /product="uncharacterized protein LOC143305517"
                     /calculated_mol_wt=51052
     Region          127..274
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..461
                     /gene="LOC143305517"
                     /coded_by="XM_076689536.1:2163..3548"
                     /db_xref="GeneID:143305517"
ORIGIN      
        1 mttsfsdllr eqeevagwiq rfwanvcklg kdkltgavle qrqgllnryw dtflsghrkl
       61 mrckeassss yvkedafsvt eeayldaafm ishhlkelps pcsshvqqsn iappphpqlp
      121 kidlpkfsgd plqwesfrdl fkslvhdvsh lpdvqkllyl kssltgeaae virntpitds
      181 gfrgawddle arygnirlls fshhralllc ppalqqsage lkrlldtfrq airayvtlrk
      241 pvtssdewfv yllsqkldkt thlawetsla dsreiprfqq lsqflenriq algaagmtdp
      301 slhavsptss keikpktkgg asakgvnsnv laaasksppa rkcpgcsgth glgfcykfka
      361 lspakrkerv qqlkaclscl nvghevgkcp skqvclacsk rhltllheal tappasapgr
      421 eggqpareda astsddatqc ewpsqalrtm rldtrperyh l
//
LOCUS       XP_076545652             161 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609672 [Osmia lignaria lignaria].
ACCESSION   XP_076545652
VERSION     XP_076545652.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689537.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="uncharacterized protein LOC117609672"
                     /calculated_mol_wt=18893
     CDS             1..161
                     /gene="LOC117609672"
                     /coded_by="XM_076689537.1:105..590"
                     /db_xref="GeneID:117609672"
ORIGIN      
        1 meskmektdn vedkkaedae pllqlqqqvq qlqlqpqqql qpqselllql qqllqqqslq
       61 mqqnqtelat lrqqvqelge rqhaglipqp qhqlkhqpph qsphqpphqp phqppqpqhq
      121 pthptyhspl aglygpeqpt ayrseqrlfr qrrrdarrlw r
//
LOCUS       XP_076545653             567 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305519 [Osmia lignaria lignaria].
ACCESSION   XP_076545653
VERSION     XP_076545653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689538.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..567
                     /product="uncharacterized protein LOC143305519"
                     /calculated_mol_wt=65484
     Region          322..512
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(378,412,422,424)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..567
                     /gene="LOC143305519"
                     /coded_by="XM_076689538.1:59..1762"
                     /db_xref="GeneID:143305519"
ORIGIN      
        1 mesdaivegf ktsvekrgli ystliadgds svykkiidad pykaqirvkk iectnhllrn
       61 fcrkmkevvk mttpgpfrri vessirrmra divkaaeyry sqqvttaeki knlyadihnv
      121 pshvfgehad csklqyfcdg tskkdekniv pelmkmgvye qikdilrpll ahaesllyns
      181 nnnavesfng iiakytggkr lnfgergsyt grcaaavlqy ntkqvlsrln tamnkeppvi
      241 lrkienrrkk enekysekrk ekkitkhvrk qfrsekdsny epnaekpdma nniyeleqkk
      301 hmemlqnwqn rrdaieeeti gqaknprwln yksklltasn fgricrrrig tlcgnsvksl
      361 vyprlisapa vqygqecekf areqlsvcig veikecglfi dsaipflgas pdglideegi
      421 veikcpktae nllpeeaiks vavirrifad aegnelnrnh yyyyqvqgql hvtgrkyclf
      481 ciwtrkglky irvdrddnfw qknmepkliq fyllcmlpel idsrynrgmp irepqfitee
      541 raslekrkrs lsesstniat vrrrtal
//
LOCUS       XP_034185022             520 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic triacylglycerol lipase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185022
VERSION     XP_034185022.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..520
                     /product="pancreatic triacylglycerol lipase isoform X1"
                     /calculated_mol_wt=58539
     Region          79..380
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            196..200
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(198,225,313)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(288..289,310..311)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..520
                     /gene="LOC117606563"
                     /coded_by="XM_034329131.2:624..2186"
                     /db_xref="GeneID:117606563"
ORIGIN      
        1 mqsklvrqgl vfliliglwv ieakrsgkll tlrnrpllrr tdinrqdlkp kicydlvgcf
       61 adppprltlk rppehpnviq trfllytrtr respdslqyg ddftsilhsr fntskplkvi
      121 ihgykgsgsd vgaillvqal lnledtsvlv ldwtrgaatt yqaavantel vgrqlglvll
      181 daihlgtlpk nihiigfslg ahvagcasei lkkrnillgr itgldpaspf frnhlfreks
      241 rkldatdaql vdiihtdgse dfadgfgllk plghidffpn ggreqpgcvd vknsvvvshl
      301 neemltkeia cshlrawslf lesmhmtnes ckfnawpcpq grnsyingic fpmestmwsq
      361 emgynanlgp lgiyylptre eepfcgqslr asvtvsdegt ktsgmlflkm eqrnstiifk
      421 lqytatkrrn kpmtfyniaa akfqflsnnq iairaqiwyq anrdeenenv ikkpnsdtlf
      481 idkiaiedrk nnrweycgsn tsinlhgrtv llqrgecnvl
//
LOCUS       XP_034185024             491 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic triacylglycerol lipase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185024
VERSION     XP_034185024.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329133.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..491
                     /product="pancreatic triacylglycerol lipase isoform X2"
                     /calculated_mol_wt=55727
     Region          79..351
                     /region_name="alpha/beta hydrolases"
                     /note="A functionally diverse superfamily containing
                     proteases, lipases, peroxidases, esterases, epoxide
                     hydrolases and dehalogenases. The catalytic apparatus
                     typically involves three residues (catalytic triad): a
                     serine, a glutamate or aspartate and a...; cl21494"
                     /db_xref="CDD:473884"
     Site            order(169,284)
                     /site_type="other"
                     /note="catalytic triad"
                     /db_xref="CDD:238382"
     CDS             1..491
                     /gene="LOC117606563"
                     /coded_by="XM_034329133.2:628..2103"
                     /db_xref="GeneID:117606563"
ORIGIN      
        1 mqsklvrqgl vfliliglwv ieakrsgkll tlrnrpllrr tdinrqdlkp kicydlvgcf
       61 adppprltlk rppehpnviq trfllytrtr respdslqyg ddftsilhsr fntskplkvi
      121 ihgykgsgsd vgaillvqal lnltqaswfs tgpeelrrli kqqwqtpssl gahvagcase
      181 ilkkrnillg ritgldpasp ffrnhlfrek srkldatdaq lvdiihtdgs edfadgfgll
      241 kplghidffp nggreqpgcv dvknsvvvsh lneemltkei acshlrawsl flesmhmtne
      301 sckfnawpcp qgrnsyingi cfpmestmws qemgynanlg plgiyylptr eeepfcgqsl
      361 rasvtvsdeg tktsgmlflk meqrnstiif klqytatkrr nkpmtfynia aakfqflsnn
      421 qiairaqiwy qanrdeenen vikkpnsdtl fidkiaiedr knnrweycgs ntsinlhgrt
      481 vllqrgecnv l
//
LOCUS       XP_034185025             293 aa            linear   INV 03-SEP-2025
DEFINITION  synthesis of cytochrome c oxidase [Osmia lignaria lignaria].
ACCESSION   XP_034185025
VERSION     XP_034185025.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329134.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="synthesis of cytochrome c oxidase"
                     /calculated_mol_wt=33367
     Region          113..254
                     /region_name="SCO"
                     /note="SCO (an acronym for Synthesis of Cytochrome c
                     Oxidase) family; composed of proteins similar to Sco1, a
                     membrane-anchored protein possessing a soluble domain with
                     a TRX fold. Members of this family are required for the
                     proper assembly of cytochrome c...; cd02968"
                     /db_xref="CDD:239266"
     Site            order(146,150,236)
                     /site_type="other"
                     /note="Cu(I) binding site [ion binding]"
                     /db_xref="CDD:239266"
     CDS             1..293
                     /gene="Scox"
                     /coded_by="XM_034329134.2:88..969"
                     /db_xref="GeneID:117606564"
ORIGIN      
        1 mtirmmsnlm nqsvfllnrs siqilkknlp nfrqyyaqir cintsvayyq snnvgslpqk
       61 kksifeknpi twksaivtgt igiallsyly ylqdvkdkqi ererrrelgk aaiggkfelv
      121 dpegktvksd dflgqwlliy fgfthcpdic pdelekmkkt vylletehki kvqplfisvd
      181 pdrdtpeivg kyvkefsdkf igltgtkeqv akvckayrvy yssgpkdqda dyivdhtiii
      241 ylidpdglfv dyyglthtae qiaqsvaink vkydsvksds wvpslslksp lpa
//
LOCUS       XP_076545654             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305520 [Osmia lignaria lignaria].
ACCESSION   XP_076545654
VERSION     XP_076545654.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689539.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC143305520"
                     /calculated_mol_wt=16896
     CDS             1..150
                     /gene="LOC143305520"
                     /coded_by="XM_076689539.1:317..769"
                     /db_xref="GeneID:143305520"
ORIGIN      
        1 mcyireqpte rdtsriellm nltarlcmrs tcyrldvgiv cmqcaleaal gaariydmve
       61 vhyplptayp pgacdicsap lmesqaayhc eeciaaiime meyqranqpi pppaivptwy
      121 terqirsrqp gprrtlrlht avssitnhht
//
LOCUS       XP_034186803             105 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607335 [Osmia lignaria lignaria].
ACCESSION   XP_034186803
VERSION     XP_034186803.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330912.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186803.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="uncharacterized protein LOC117607335"
                     /calculated_mol_wt=11832
     CDS             1..105
                     /gene="LOC117607335"
                     /coded_by="XM_034330912.2:472..789"
                     /db_xref="GeneID:117607335"
ORIGIN      
        1 maedkmdvdk qieekkeere kkveveekea enkqentkke evpkrqeeql saseikkmvk
       61 kmiygghrgg rhggrggrwg grrggrggrr gggrggnrpv ifnif
//
LOCUS       XP_034190493             151 aa            linear   INV 03-SEP-2025
DEFINITION  MYC associated factor X isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190493
VERSION     XP_034190493.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334602.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="MYC associated factor X isoform X1"
                     /calculated_mol_wt=16996
     Region          16..85
                     /region_name="bHLHzip_Max"
                     /note="basic Helix-Loop-Helix-zipper (bHLHzip) domain
                     found in protein Max and similar proteins; cd11406"
                     /db_xref="CDD:381412"
     Site            order(17,19..21,23..25,27..28,32,52..53)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381412"
     Site            order(31,34..35,37..38,41..43,54,57..58,61,63..65,67..68,
                     70..71,74,77..78,81..82,84..85)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381412"
     CDS             1..151
                     /gene="Max"
                     /coded_by="XM_034334602.2:122..577"
                     /db_xref="GeneID:117608876"
ORIGIN      
        1 msdddrdidi esdaekrahh nalerkrrdh ikdsfsslrd svpalqgekv asraqilkka
       61 aeyiqfmrrk nsshqqdidd lkrqnnlles qiralekakm tgnfaaetce vtksegvpms
      121 gyndtesess dseasrtvrq tkklkvgglh h
//
LOCUS       XP_034190492             171 aa            linear   INV 03-SEP-2025
DEFINITION  MYC associated factor X isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190492
VERSION     XP_034190492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="MYC associated factor X isoform X2"
                     /calculated_mol_wt=19365
     Region          36..105
                     /region_name="bHLHzip_Max"
                     /note="basic Helix-Loop-Helix-zipper (bHLHzip) domain
                     found in protein Max and similar proteins; cd11406"
                     /db_xref="CDD:381412"
     Site            order(37,39..41,43..45,47..48,52,72..73)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381412"
     Site            order(51,54..55,57..58,61..63,74,77..78,81,83..85,87..88,
                     90..91,94,97..98,101..102,104..105)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381412"
     CDS             1..171
                     /gene="Max"
                     /coded_by="XM_034334601.2:52..567"
                     /db_xref="GeneID:117608876"
ORIGIN      
        1 msdddrdidi esdegddsds rqrhsnntqy ysqaekrahh nalerkrrdh ikdsfsslrd
       61 svpalqgekv asraqilkka aeyiqfmrrk nsshqqdidd lkrqnnlles qiralekakm
      121 tgnfaaetce vtksegvpms gyndtesess dseasrtvrq tkklkvgglh h
//
LOCUS       XP_034184905            1008 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184905
VERSION     XP_034184905.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329014.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184905.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1008
                     /product="phosphodiesterase dunce isoform X1"
                     /calculated_mol_wt=112401
     Region          428..546
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          687..927
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..1008
                     /gene="dnc"
                     /coded_by="XM_034329014.2:502..3528"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mddddtdekl tlinklshke ckakllvtsk ftsrssrlea kqrnstedsd tmecasenev
       61 enlpkdedas rpivdekisr ipaierhsld vpeeaesllm nrqqsmtksr lrvseeetrt
      121 tkfagtrlgl fnrspqssss qeeyepevks ltklqrqqqq kqqhlltats ggqitarsme
      181 slrtsrnfls addrysedsk smeslslsse ahvtssksqy ksfltastkd vrrivsvesk
      241 ssenlqrees lnaevrrglf antglerkip qhlslpppss vfsltspggs drkitilsph
      301 spiqsmefqf stqtlktrrk kaivlprlvl prsesevfld fdvengastl dggaggggga
      361 spsaglvlqt lpqrresfly rsdsdfemsp ksmsrnssia serfketelp veraiftdqy
      421 shgedlivtp faqilaslrs vrnnflsltn vptnksrrss gaqgsstpqp rivapgeeny
      481 mklavetmee ldwcldqlet iqthrsvsdm aslkfkrmln kelshfsess ksgnqiseyi
      541 cstfldkqqe ldlpslried avaaagsada raakkkdrvq rgpaamshis gvkrplthtn
      601 sftgervply gvetpheeel gkllsdidkw gidifrigel snnrpltcva ytafqsrdll
      661 kslaippktf vtfmmtledh yvkdnpfhns lhaadvtqst htllntpale svftpleita
      721 alfaatihdv dhpgltnqfl inssselalm yndesvlenh hlavafkllq negcdifvnm
      781 tkkqrqtlrk mvidmvlstd mskhmsllad lktmvetkkv agsgvllldn ytdriqvlen
      841 lvhcadlsnp tkplplyerw vgllmeeffl qgdrereqnm dispmcdrhs atieksqvgf
      901 idyivhplwe twadlvhpda qdildtleen rdwyqskisp sppsddqpqq qgnepqseri
      961 hfqvtleegd etgeatetge sggaaqtttl sseegggete enaaeagm
//
LOCUS       XP_034184908            1001 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184908
VERSION     XP_034184908.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329017.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184908.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1001
                     /product="phosphodiesterase dunce isoform X2"
                     /calculated_mol_wt=111546
     Region          421..539
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          680..920
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..1001
                     /gene="dnc"
                     /coded_by="XM_034329017.2:502..3507"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mddddtdekl tlinklshke ckakllvtsk ftsrssrlea kqrnstedsd tmecasenev
       61 enlpkdedas rpivdekisr ipaierhsld vpeeaesllm nrqqsmtksr lrvseeetrt
      121 tkfagtrlgl fnrspqssss qeeyepevks ltklqrqqqq kqqhlltats ggqitarsme
      181 slrtsrnfls addrysedsk smeslslsse ahvtssksqy ksfltastkd vrrivsvesk
      241 ssenlqrees lnaevrrglf antglerkip qhlslpppss vfsltspggs drkitilsph
      301 spiqsmefqf stqtlktrrk kaivlprlvl prsesevfld fdvengastl dggaggggga
      361 spsaglvlqt lpqrresfly rsdsdfemsp ksmsrnssia serfketelp verahgedli
      421 vtpfaqilas lrsvrnnfls ltnvptnksr rssgaqgsst pqprivapge enymklavet
      481 meeldwcldq letiqthrsv sdmaslkfkr mlnkelshfs essksgnqis eyicstfldk
      541 qqeldlpslr iedavaaags adaraakkkd rvqrgpaams hisgvkrplt htnsftgerv
      601 plygvetphe eelgkllsdi dkwgidifri gelsnnrplt cvaytafqsr dllkslaipp
      661 ktfvtfmmtl edhyvkdnpf hnslhaadvt qsthtllntp alesvftple itaalfaati
      721 hdvdhpgltn qflinsssel almyndesvl enhhlavafk llqnegcdif vnmtkkqrqt
      781 lrkmvidmvl stdmskhmsl ladlktmvet kkvagsgvll ldnytdriqv lenlvhcadl
      841 snptkplply erwvgllmee fflqgdrere qnmdispmcd rhsatieksq vgfidyivhp
      901 lwetwadlvh pdaqdildtl eenrdwyqsk ispsppsddq pqqqgnepqs erihfqvtle
      961 egdetgeate tgesggaaqt ttlsseeggg eteenaaeag m
//
LOCUS       XP_034184909             995 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034184909
VERSION     XP_034184909.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329018.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184909.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..995
                     /product="phosphodiesterase dunce isoform X3"
                     /calculated_mol_wt=110846
     Region          415..533
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          674..914
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..995
                     /gene="dnc"
                     /coded_by="XM_034329018.2:502..3489"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mddddtdekl tlinklshke ckakllvtsk ftsrssrlea kqrnstedsd tmecasenev
       61 enlpkdedas rpivdekisr ipaierhsld vpeeaesllm nrqqsmtksr lrvseeetrt
      121 tkfagtrlgl fnrspqssss qeeyepevks ltklqrqqqq kqqhlltats ggqitarsme
      181 slrtsrnfls addrysedsk smeslslsse ahvtssksqy ksfltastkd vrrivsvesk
      241 ssenlqrees lnaevrrglf antglerkip qhlslpppss vfsltspggs drkitilsph
      301 spiqsmefqf stqtlktrrk kaivlpsfdv engastldgg agggggasps aglvlqtlpq
      361 rresflyrsd sdfemspksm srnssiaser fketelpver aiftdqyshg edlivtpfaq
      421 ilaslrsvrn nflsltnvpt nksrrssgaq gsstpqpriv apgeenymkl avetmeeldw
      481 cldqletiqt hrsvsdmasl kfkrmlnkel shfsessksg nqiseyicst fldkqqeldl
      541 pslriedava aagsadaraa kkkdrvqrgp aamshisgvk rplthtnsft gervplygve
      601 tpheeelgkl lsdidkwgid ifrigelsnn rpltcvayta fqsrdllksl aippktfvtf
      661 mmtledhyvk dnpfhnslha advtqsthtl lntpalesvf tpleitaalf aatihdvdhp
      721 gltnqflins sselalmynd esvlenhhla vafkllqneg cdifvnmtkk qrqtlrkmvi
      781 dmvlstdmsk hmslladlkt mvetkkvags gvllldnytd riqvlenlvh cadlsnptkp
      841 lplyerwvgl lmeefflqgd rereqnmdis pmcdrhsati eksqvgfidy ivhplwetwa
      901 dlvhpdaqdi ldtleenrdw yqskispspp sddqpqqqgn epqserihfq vtleegdetg
      961 eatetgesgg aaqtttlsse egggeteena aeagm
//
LOCUS       XP_034184910             990 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034184910
VERSION     XP_034184910.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329019.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184910.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..990
                     /product="phosphodiesterase dunce isoform X4"
                     /calculated_mol_wt=110177
     Region          410..528
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          669..909
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..990
                     /gene="dnc"
                     /coded_by="XM_034329019.2:502..3474"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mddddtdekl tlinklshke ckakllvtsk ftsrssrlea kqrnstedsd tmecasenev
       61 enlpkdedas rpivdekisr ipaierhsld vpeeaesllm nrqqsmtksr lrvseeetrt
      121 tkfagtrlgl fnrspqssss qeeyepevks ltklqrqqqq kqqhlltats ggqitarsme
      181 slrtsrnfls addrysedsk smeslslsse ahvtssksqy ksfltastkd vrrivsvesk
      241 ssenlqrees lnaevrrglf antglerkip qhlslpppss vfsltspggs drkitilsph
      301 spiqsmefqf stqtlktrrk kaivlprlvl prsesevfld fdvengastl dggaggggga
      361 spsaglvlqt lpqrresfly rsdsdfemsp ksmsrnssia seshgedliv tpfaqilasl
      421 rsvrnnflsl tnvptnksrr ssgaqgsstp qprivapgee nymklavetm eeldwcldql
      481 etiqthrsvs dmaslkfkrm lnkelshfse ssksgnqise yicstfldkq qeldlpslri
      541 edavaaagsa daraakkkdr vqrgpaamsh isgvkrplth tnsftgervp lygvetphee
      601 elgkllsdid kwgidifrig elsnnrpltc vaytafqsrd llkslaippk tfvtfmmtle
      661 dhyvkdnpfh nslhaadvtq sthtllntpa lesvftplei taalfaatih dvdhpgltnq
      721 flinsssela lmyndesvle nhhlavafkl lqnegcdifv nmtkkqrqtl rkmvidmvls
      781 tdmskhmsll adlktmvetk kvagsgvlll dnytdriqvl enlvhcadls nptkplplye
      841 rwvgllmeef flqgdrereq nmdispmcdr hsatieksqv gfidyivhpl wetwadlvhp
      901 daqdildtle enrdwyqski spsppsddqp qqqgnepqse rihfqvtlee gdetgeatet
      961 gesggaaqtt tlsseeggge teenaaeagm
//
LOCUS       XP_034184911             749 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034184911
VERSION     XP_034184911.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..749
                     /product="phosphodiesterase dunce isoform X5"
                     /calculated_mol_wt=84001
     Region          169..287
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          428..668
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..749
                     /gene="dnc"
                     /coded_by="XM_034329020.2:2274..4523"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mtedatmitf lfliwmmvrs kepqdekqdt rsdsfskdnr ervdsrrnsk ettsiglphi
       61 drrrhrlrrh srryyvdrnr sfdvengast ldggaggggg aspsaglvlq tlpqrresfl
      121 yrsdsdfems pksmsrnssi aserfketel pveraiftdq yshgedlivt pfaqilaslr
      181 svrnnflslt nvptnksrrs sgaqgsstpq privapgeen ymklavetme eldwcldqle
      241 tiqthrsvsd maslkfkrml nkelshfses sksgnqisey icstfldkqq eldlpslrie
      301 davaaagsad araakkkdrv qrgpaamshi sgvkrpltht nsftgervpl ygvetpheee
      361 lgkllsdidk wgidifrige lsnnrpltcv aytafqsrdl lkslaippkt fvtfmmtled
      421 hyvkdnpfhn slhaadvtqs thtllntpal esvftpleit aalfaatihd vdhpgltnqf
      481 linssselal myndesvlen hhlavafkll qnegcdifvn mtkkqrqtlr kmvidmvlst
      541 dmskhmslla dlktmvetkk vagsgvllld nytdriqvle nlvhcadlsn ptkplplyer
      601 wvgllmeeff lqgdrereqn mdispmcdrh satieksqvg fidyivhplw etwadlvhpd
      661 aqdildtlee nrdwyqskis psppsddqpq qqgnepqser ihfqvtleeg detgeatetg
      721 esggaaqttt lsseeggget eenaaeagm
//
LOCUS       XP_076545655             747 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076545655
VERSION     XP_076545655.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689540.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..747
                     /product="phosphodiesterase dunce isoform X6"
                     /calculated_mol_wt=82462
     Region          167..285
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          426..666
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..747
                     /gene="dnc"
                     /coded_by="XM_076689540.1:16..2259"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mmkcddrkre mfsrracses gphpgipgtg sgpggggghl wgkrnqgrts apvvghargp
       61 aiilrtrrsw pvapmhcrsf dvengastld ggagggggas psaglvlqtl pqrresflyr
      121 sdsdfemspk smsrnssias erfketelpv eraiftdqys hgedlivtpf aqilaslrsv
      181 rnnflsltnv ptnksrrssg aqgsstpqpr ivapgeenym klavetmeel dwcldqleti
      241 qthrsvsdma slkfkrmlnk elshfsessk sgnqiseyic stfldkqqel dlpslrieda
      301 vaaagsadar aakkkdrvqr gpaamshisg vkrplthtns ftgervplyg vetpheeelg
      361 kllsdidkwg idifrigels nnrpltcvay tafqsrdllk slaippktfv tfmmtledhy
      421 vkdnpfhnsl haadvtqsth tllntpales vftpleitaa lfaatihdvd hpgltnqfli
      481 nssselalmy ndesvlenhh lavafkllqn egcdifvnmt kkqrqtlrkm vidmvlstdm
      541 skhmslladl ktmvetkkva gsgvllldny tdriqvlenl vhcadlsnpt kplplyerwv
      601 gllmeefflq gdrereqnmd ispmcdrhsa tieksqvgfi dyivhplwet wadlvhpdaq
      661 dildtleenr dwyqskisps ppsddqpqqq gnepqserih fqvtleegde tgeatetges
      721 ggaaqtttls seegggetee naaeagm
//
LOCUS       XP_034184912             726 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034184912
VERSION     XP_034184912.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..726
                     /product="phosphodiesterase dunce isoform X7"
                     /calculated_mol_wt=80466
     Region          146..264
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          405..645
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..726
                     /gene="dnc"
                     /coded_by="XM_034329021.2:480..2660"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 msdyailvph evssrlarrd sgtmrvtlpv tsfrrhsgcv smlsrakphp klvyvavpfd
       61 vengastldg gagggggasp saglvlqtlp qrresflyrs dsdfemspks msrnssiase
      121 rfketelpve raiftdqysh gedlivtpfa qilaslrsvr nnflsltnvp tnksrrssga
      181 qgsstpqpri vapgeenymk lavetmeeld wcldqletiq thrsvsdmas lkfkrmlnke
      241 lshfsessks gnqiseyics tfldkqqeld lpslriedav aaagsadara akkkdrvqrg
      301 paamshisgv krplthtnsf tgervplygv etpheeelgk llsdidkwgi difrigelsn
      361 nrpltcvayt afqsrdllks laippktfvt fmmtledhyv kdnpfhnslh aadvtqstht
      421 llntpalesv ftpleitaal faatihdvdh pgltnqflin ssselalmyn desvlenhhl
      481 avafkllqne gcdifvnmtk kqrqtlrkmv idmvlstdms khmslladlk tmvetkkvag
      541 sgvllldnyt driqvlenlv hcadlsnptk plplyerwvg llmeefflqg drereqnmdi
      601 spmcdrhsat ieksqvgfid yivhplwetw adlvhpdaqd ildtleenrd wyqskispsp
      661 psddqpqqqg nepqserihf qvtleegdet geatetgesg gaaqtttlss eegggeteen
      721 aaeagm
//
LOCUS       XP_034184913             713 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034184913
VERSION     XP_034184913.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329022.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..713
                     /product="phosphodiesterase dunce isoform X8"
                     /calculated_mol_wt=78628
     Region          133..251
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          392..632
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..713
                     /gene="dnc"
                     /coded_by="XM_034329022.2:735..2876"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 msslqvpelr lpagdgggsg gkgtaplllv pqssvprrrh swicgfdven gastldggag
       61 ggggaspsag lvlqtlpqrr esflyrsdsd femspksmsr nssiaserfk etelpverai
      121 ftdqyshged livtpfaqil aslrsvrnnf lsltnvptnk srrssgaqgs stpqprivap
      181 geenymklav etmeeldwcl dqletiqthr svsdmaslkf krmlnkelsh fsessksgnq
      241 iseyicstfl dkqqeldlps lriedavaaa gsadaraakk kdrvqrgpaa mshisgvkrp
      301 lthtnsftge rvplygvetp heeelgklls didkwgidif rigelsnnrp ltcvaytafq
      361 srdllkslai ppktfvtfmm tledhyvkdn pfhnslhaad vtqsthtlln tpalesvftp
      421 leitaalfaa tihdvdhpgl tnqflinsss elalmyndes vlenhhlava fkllqnegcd
      481 ifvnmtkkqr qtlrkmvidm vlstdmskhm slladlktmv etkkvagsgv llldnytdri
      541 qvlenlvhca dlsnptkplp lyerwvgllm eefflqgdre reqnmdispm cdrhsatiek
      601 sqvgfidyiv hplwetwadl vhpdaqdild tleenrdwyq skispsppsd dqpqqqgnep
      661 qserihfqvt leegdetgea tetgesggaa qtttlsseeg ggeteenaae agm
//
LOCUS       XP_034184914             698 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034184914
VERSION     XP_034184914.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329023.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..698
                     /product="phosphodiesterase dunce isoform X9"
                     /calculated_mol_wt=77706
     Region          118..236
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          377..617
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..698
                     /gene="dnc"
                     /coded_by="XM_034329023.2:881..2977"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 merlsvaetl rrkdrrrrrg wtvvvghevr fdvengastl dggaggggga spsaglvlqt
       61 lpqrresfly rsdsdfemsp ksmsrnssia serfketelp veraiftdqy shgedlivtp
      121 faqilaslrs vrnnflsltn vptnksrrss gaqgsstpqp rivapgeeny mklavetmee
      181 ldwcldqlet iqthrsvsdm aslkfkrmln kelshfsess ksgnqiseyi cstfldkqqe
      241 ldlpslried avaaagsada raakkkdrvq rgpaamshis gvkrplthtn sftgervply
      301 gvetpheeel gkllsdidkw gidifrigel snnrpltcva ytafqsrdll kslaippktf
      361 vtfmmtledh yvkdnpfhns lhaadvtqst htllntpale svftpleita alfaatihdv
      421 dhpgltnqfl inssselalm yndesvlenh hlavafkllq negcdifvnm tkkqrqtlrk
      481 mvidmvlstd mskhmsllad lktmvetkkv agsgvllldn ytdriqvlen lvhcadlsnp
      541 tkplplyerw vgllmeeffl qgdrereqnm dispmcdrhs atieksqvgf idyivhplwe
      601 twadlvhpda qdildtleen rdwyqskisp sppsddqpqq qgnepqseri hfqvtleegd
      661 etgeatetge sggaaqtttl sseegggete enaaeagm
//
LOCUS       XP_076545656             697 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_076545656
VERSION     XP_076545656.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689541.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="phosphodiesterase dunce isoform X10"
                     /calculated_mol_wt=77194
     Region          117..235
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          376..616
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..697
                     /gene="dnc"
                     /coded_by="XM_076689541.1:16..2109"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mgqdwcarrr krsssgccpc gprvhpsssf dvengastld ggagggggas psaglvlqtl
       61 pqrresflyr sdsdfemspk smsrnssias erfketelpv eraiftdqys hgedlivtpf
      121 aqilaslrsv rnnflsltnv ptnksrrssg aqgsstpqpr ivapgeenym klavetmeel
      181 dwcldqleti qthrsvsdma slkfkrmlnk elshfsessk sgnqiseyic stfldkqqel
      241 dlpslrieda vaaagsadar aakkkdrvqr gpaamshisg vkrplthtns ftgervplyg
      301 vetpheeelg kllsdidkwg idifrigels nnrpltcvay tafqsrdllk slaippktfv
      361 tfmmtledhy vkdnpfhnsl haadvtqsth tllntpales vftpleitaa lfaatihdvd
      421 hpgltnqfli nssselalmy ndesvlenhh lavafkllqn egcdifvnmt kkqrqtlrkm
      481 vidmvlstdm skhmslladl ktmvetkkva gsgvllldny tdriqvlenl vhcadlsnpt
      541 kplplyerwv gllmeefflq gdrereqnmd ispmcdrhsa tieksqvgfi dyivhplwet
      601 wadlvhpdaq dildtleenr dwyqskisps ppsddqpqqq gnepqserih fqvtleegde
      661 tgeatetges ggaaqtttls seegggetee naaeagm
//
LOCUS       XP_034184915             696 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_034184915
VERSION     XP_034184915.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329024.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..696
                     /product="phosphodiesterase dunce isoform X11"
                     /calculated_mol_wt=77252
     Region          116..234
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          375..615
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..696
                     /gene="dnc"
                     /coded_by="XM_034329024.2:504..2594"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mqaeqgsige lrgyhnlrsr rhtlanvrfd vengastldg gagggggasp saglvlqtlp
       61 qrresflyrs dsdfemspks msrnssiase rfketelpve raiftdqysh gedlivtpfa
      121 qilaslrsvr nnflsltnvp tnksrrssga qgsstpqpri vapgeenymk lavetmeeld
      181 wcldqletiq thrsvsdmas lkfkrmlnke lshfsessks gnqiseyics tfldkqqeld
      241 lpslriedav aaagsadara akkkdrvqrg paamshisgv krplthtnsf tgervplygv
      301 etpheeelgk llsdidkwgi difrigelsn nrpltcvayt afqsrdllks laippktfvt
      361 fmmtledhyv kdnpfhnslh aadvtqstht llntpalesv ftpleitaal faatihdvdh
      421 pgltnqflin ssselalmyn desvlenhhl avafkllqne gcdifvnmtk kqrqtlrkmv
      481 idmvlstdms khmslladlk tmvetkkvag sgvllldnyt driqvlenlv hcadlsnptk
      541 plplyerwvg llmeefflqg drereqnmdi spmcdrhsat ieksqvgfid yivhplwetw
      601 adlvhpdaqd ildtleenrd wyqskispsp psddqpqqqg nepqserihf qvtleegdet
      661 geatetgesg gaaqtttlss eegggeteen aaeagm
//
LOCUS       XP_076545657             689 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X12 [Osmia lignaria lignaria].
ACCESSION   XP_076545657
VERSION     XP_076545657.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689542.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..689
                     /product="phosphodiesterase dunce isoform X12"
                     /calculated_mol_wt=76397
     Region          109..227
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          368..608
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..689
                     /gene="dnc"
                     /coded_by="XM_076689542.1:503..2572"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mqaeqgsige lrgyhnlrsr rhtlanvrfd vengastldg gagggggasp saglvlqtlp
       61 qrresflyrs dsdfemspks msrnssiase rfketelpve rahgedlivt pfaqilaslr
      121 svrnnflslt nvptnksrrs sgaqgsstpq privapgeen ymklavetme eldwcldqle
      181 tiqthrsvsd maslkfkrml nkelshfses sksgnqisey icstfldkqq eldlpslrie
      241 davaaagsad araakkkdrv qrgpaamshi sgvkrpltht nsftgervpl ygvetpheee
      301 lgkllsdidk wgidifrige lsnnrpltcv aytafqsrdl lkslaippkt fvtfmmtled
      361 hyvkdnpfhn slhaadvtqs thtllntpal esvftpleit aalfaatihd vdhpgltnqf
      421 linssselal myndesvlen hhlavafkll qnegcdifvn mtkkqrqtlr kmvidmvlst
      481 dmskhmslla dlktmvetkk vagsgvllld nytdriqvle nlvhcadlsn ptkplplyer
      541 wvgllmeeff lqgdrereqn mdispmcdrh satieksqvg fidyivhplw etwadlvhpd
      601 aqdildtlee nrdwyqskis psppsddqpq qqgnepqser ihfqvtleeg detgeatetg
      661 esggaaqttt lsseeggget eenaaeagm
//
LOCUS       XP_034184916             661 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X13 [Osmia lignaria lignaria].
ACCESSION   XP_034184916
VERSION     XP_034184916.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329025.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="phosphodiesterase dunce isoform X13"
                     /calculated_mol_wt=72892
     Region          81..199
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          340..580
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..661
                     /gene="dnc"
                     /coded_by="XM_034329025.2:2567..4552"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 msdssgglmd fsgsgsglkr cgagmsspsl gaggsignsg glprtlsasa lrlrpsrkal
       61 fweqcfespp krdfhgedli vtpfaqilas lrsvrnnfls ltnvptnksr rssgaqgsst
      121 pqprivapge enymklavet meeldwcldq letiqthrsv sdmaslkfkr mlnkelshfs
      181 essksgnqis eyicstfldk qqeldlpslr iedavaaags adaraakkkd rvqrgpaams
      241 hisgvkrplt htnsftgerv plygvetphe eelgkllsdi dkwgidifri gelsnnrplt
      301 cvaytafqsr dllkslaipp ktfvtfmmtl edhyvkdnpf hnslhaadvt qsthtllntp
      361 alesvftple itaalfaati hdvdhpgltn qflinsssel almyndesvl enhhlavafk
      421 llqnegcdif vnmtkkqrqt lrkmvidmvl stdmskhmsl ladlktmvet kkvagsgvll
      481 ldnytdriqv lenlvhcadl snptkplply erwvgllmee fflqgdrere qnmdispmcd
      541 rhsatieksq vgfidyivhp lwetwadlvh pdaqdildtl eenrdwyqsk ispsppsddq
      601 pqqqgnepqs erihfqvtle egdetgeate tgesggaaqt ttlsseeggg eteenaaeag
      661 m
//
LOCUS       XP_034184919             661 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X13 [Osmia lignaria lignaria].
ACCESSION   XP_034184919
VERSION     XP_034184919.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329028.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="phosphodiesterase dunce isoform X13"
                     /calculated_mol_wt=72892
     Region          81..199
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          340..580
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..661
                     /gene="dnc"
                     /coded_by="XM_034329028.2:2502..4487"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 msdssgglmd fsgsgsglkr cgagmsspsl gaggsignsg glprtlsasa lrlrpsrkal
       61 fweqcfespp krdfhgedli vtpfaqilas lrsvrnnfls ltnvptnksr rssgaqgsst
      121 pqprivapge enymklavet meeldwcldq letiqthrsv sdmaslkfkr mlnkelshfs
      181 essksgnqis eyicstfldk qqeldlpslr iedavaaags adaraakkkd rvqrgpaams
      241 hisgvkrplt htnsftgerv plygvetphe eelgkllsdi dkwgidifri gelsnnrplt
      301 cvaytafqsr dllkslaipp ktfvtfmmtl edhyvkdnpf hnslhaadvt qsthtllntp
      361 alesvftple itaalfaati hdvdhpgltn qflinsssel almyndesvl enhhlavafk
      421 llqnegcdif vnmtkkqrqt lrkmvidmvl stdmskhmsl ladlktmvet kkvagsgvll
      481 ldnytdriqv lenlvhcadl snptkplply erwvgllmee fflqgdrere qnmdispmcd
      541 rhsatieksq vgfidyivhp lwetwadlvh pdaqdildtl eenrdwyqsk ispsppsddq
      601 pqqqgnepqs erihfqvtle egdetgeate tgesggaaqt ttlsseeggg eteenaaeag
      661 m
//
LOCUS       XP_034184920             532 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase dunce isoform X14 [Osmia lignaria lignaria].
ACCESSION   XP_034184920
VERSION     XP_034184920.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329029.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..532
                     /product="phosphodiesterase dunce isoform X14"
                     /calculated_mol_wt=59091
     Region          <31..70
                     /region_name="PDE4_UCR"
                     /note="Phosphodiesterase 4 upstream conserved regions
                     (UCR); pfam18100"
                     /db_xref="CDD:465648"
     Region          211..451
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..532
                     /gene="dnc"
                     /coded_by="XM_034329029.2:478..2076"
                     /db_xref="GeneID:117606508"
ORIGIN      
        1 mqtdaelatg nvddsgryfn clrtladlta masivavefk rmlnkelshf sessksgnqi
       61 seyicstfld kqqeldlpsl riedavaaag sadaraakkk drvqrgpaam shisgvkrpl
      121 thtnsftger vplygvetph eeelgkllsd idkwgidifr igelsnnrpl tcvaytafqs
      181 rdllkslaip pktfvtfmmt ledhyvkdnp fhnslhaadv tqsthtllnt palesvftpl
      241 eitaalfaat ihdvdhpglt nqflinssse lalmyndesv lenhhlavaf kllqnegcdi
      301 fvnmtkkqrq tlrkmvidmv lstdmskhms lladlktmve tkkvagsgvl lldnytdriq
      361 vlenlvhcad lsnptkplpl yerwvgllme efflqgdrer eqnmdispmc drhsatieks
      421 qvgfidyivh plwetwadlv hpdaqdildt leenrdwyqs kispsppsdd qpqqqgnepq
      481 serihfqvtl eegdetgeat etgesggaaq tttlsseegg geteenaaea gm
//
LOCUS       XP_034184921             828 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606509 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184921
VERSION     XP_034184921.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329030.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="uncharacterized protein LOC117606509 isoform X1"
                     /calculated_mol_wt=91730
     CDS             1..828
                     /gene="LOC117606509"
                     /coded_by="XM_034329030.2:208..2694"
                     /db_xref="GeneID:117606509"
ORIGIN      
        1 msveaktsct ttcasetddg dyetssegpv rkevaqnstn keafsasssk mgsqqdndte
       61 dseskkkkss ekffsllhsn vltksesmpv lksttsledt vedtrvvsse splarrrkmk
      121 mivaqifsrs sitsttlsgg srteltkeny qakkmesrtm lpvlqgidyd ppnrlkevtf
      181 helpedkdfs sdrsqtserk ktkssrlptr ddesqqhsqe ngdlidiaen phpsiksfyf
      241 ppnpqkeass aslrthsrat kdgvqrdvse lrfllqqlnv isgraralsq idpflssqrh
      301 stqcspysni sgyylhqtet ysrdtqvnds iqlisrqmid essqgirdtd yvgsrafcpa
      361 gnqmvplgrd ythyenpmkh ssryvgrnls sgltnrtvge ddsrvtlnnf hfqfgqyvek
      421 hpsgslipsv ssscyrchrn rtcvlshydd tagcstssgg gqvtsafskr adenlyksrd
      481 lvekstmksk efpldlsdnq pkpswaslmn ssskvrttcv naelladekp rshknsakns
      541 nnslhcyqdq svaasliria sdlsrpstvk clnsqaslvi skrdqdvmvs nlglvedqtt
      601 scssqvvtkh ehpkgqkkks vkdldvqcsg snlkfhqkei algqsmesfl kpsvseesna
      661 skgssnqsks iqvateekvv vvpivftdgv pepqkiplft vtrsrthlhn vkchlvklkk
      721 gtsaltqssm vsansiesce tslnrddsks kmsvscvsrs slhcrngsrt antiktlsdh
      781 rhrsdskhkr vgsmasgytq stkahseptk krvmygmrss pedfvfdt
//
LOCUS       XP_034184922             801 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606509 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184922
VERSION     XP_034184922.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329031.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..801
                     /product="uncharacterized protein LOC117606509 isoform X2"
                     /calculated_mol_wt=88830
     CDS             1..801
                     /gene="LOC117606509"
                     /coded_by="XM_034329031.2:209..2614"
                     /db_xref="GeneID:117606509"
ORIGIN      
        1 msveaktsct ttcasetddg dyetssegpv rkevaqnstn keafsasssk mgsqqdndte
       61 dseskkkkss ekffkdtrvv ssesplarrr kmkmivaqif srssitsttl sggsrteltk
      121 enyqakkmes rtmlpvlqgi dydppnrlke vtfhelpedk dfssdrsqts erkktkssrl
      181 ptrddesqqh sqengdlidi aenphpsiks fyfppnpqke assaslrths ratkdgvqrd
      241 vselrfllqq lnvisgrara lsqidpflss qrhstqcspy snisgyylhq tetysrdtqv
      301 ndsiqlisrq midessqgir dtdyvgsraf cpagnqmvpl grdythyenp mkhssryvgr
      361 nlssgltnrt vgeddsrvtl nnfhfqfgqy vekhpsgsli psvssscyrc hrnrtcvlsh
      421 yddtagcsts sgggqvtsaf skradenlyk srdlvekstm kskefpldls dnqpkpswas
      481 lmnssskvrt tcvnaellad ekprshknsa knsnnslhcy qdqsvaasli riasdlsrps
      541 tvkclnsqas lviskrdqdv mvsnlglved qttscssqvv tkhehpkgqk kksvkdldvq
      601 csgsnlkfhq keialgqsme sflkpsvsee snaskgssnq sksiqvatee kvvvvpivft
      661 dgvpepqkip lftvtrsrth lhnvkchlvk lkkgtsaltq ssmvsansie scetslnrdd
      721 skskmsvscv srsslhcrng srtantiktl sdhrhrsdsk hkrvgsmasg ytqstkahse
      781 ptkkrvmygm rsspedfvfd t
//
LOCUS       XP_034184925             340 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606511 [Osmia lignaria lignaria].
ACCESSION   XP_034184925
VERSION     XP_034184925.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="uncharacterized protein LOC117606511"
                     /calculated_mol_wt=33318
     Region          <57..>329
                     /region_name="ser_rich_anae_1"
                     /note="serine-rich protein; NF033849"
                     /db_xref="CDD:468206"
     CDS             1..340
                     /gene="LOC117606511"
                     /coded_by="XM_034329034.2:313..1335"
                     /db_xref="GeneID:117606511"
ORIGIN      
        1 mrilifclla tvafvtkaep qqqktkrgvl lgsgglggsl vgldlgnsle sagipggyts
       61 glsigtyggl sngisgltgg ygsnigvgin gglstgltag isgglstanv qsiaqqvpvv
      121 vpqqypvsrp vsatyyvrqt vpliknlpvh vsqpvpiags qsirvpvpap qsipiqqtap
      181 qsvllssgss lgsslgytgh esginsggls pslgaghipg ihlnsgidtg ltsgigsayg
      241 sglnsigsgl ghgsflnygs glglnsgygs rlsygfgsag ipsggglnsg ygsafgwtpg
      301 tglgpystgl tdnlgsgyga sygystsgsl dqsgivfrky
//
LOCUS       XP_034184924             274 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein F13E9.13, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034184924
VERSION     XP_034184924.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329033.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184924.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..274
                     /product="uncharacterized protein F13E9.13, mitochondrial"
                     /calculated_mol_wt=29529
     Region          4..270
                     /region_name="BtpA"
                     /note="Membrane biogenesis protein, BtpA/SgcQ family [Cell
                     wall/membrane/envelope biogenesis]; COG0434"
                     /db_xref="CDD:440203"
     CDS             1..274
                     /gene="LOC117606510"
                     /coded_by="XM_034329033.2:307..1131"
                     /db_xref="GeneID:117606510"
ORIGIN      
        1 mlgfhtlfnk grcsvigmvh vgalpgsply ggdtrkildn aikealiykd cyvdgilven
       61 mhdipyvgpk dispettafm tricteikra lpdnlpcgvq ilggcnkeam avakaadfqf
      121 iraegfvfsh iadegftdac agsllryrkq idaedvlila dikkkhsshs itsdvslset
      181 vkaaefflad giiltgtatg dpasaaelke vkeiakgpvl igsgvsisni gnylssdavi
      241 vgsyfkvdgk wqnpidnqkv ynfmrilqkl qnpk
//
LOCUS       XP_034184928             242 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p53 inducible nuclear protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184928
VERSION     XP_034184928.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329037.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="tumor protein p53 inducible nuclear protein
                     isoform X1"
                     /calculated_mol_wt=26682
     Region          <61..239
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..242
                     /gene="TP53INP"
                     /coded_by="XM_034329037.2:177..905"
                     /db_xref="GeneID:117606512"
ORIGIN      
        1 mlsslanyll ggnisgaqds regsnnetpe slpvmarlsq vevegddwil idravegtta
       61 leeswyvtpp acftragpvn vetspledll iehpsmsvyr ataspvapdt ppptpdapee
      121 rdveedvlpa vvsgtpaeaa slerpsprrs edeeatrrpa ihdgrpvsgr vrtekrivql
      181 rsaqkqlnyv qvlekrstqa lkrgrlersn klrevfsvkg krprrqdrlr iqnsgannnr
      241 kc
//
LOCUS       XP_034184930             242 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p53 inducible nuclear protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184930
VERSION     XP_034184930.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329039.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="tumor protein p53 inducible nuclear protein
                     isoform X1"
                     /calculated_mol_wt=26682
     Region          <61..239
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..242
                     /gene="TP53INP"
                     /coded_by="XM_034329039.2:553..1281"
                     /db_xref="GeneID:117606512"
ORIGIN      
        1 mlsslanyll ggnisgaqds regsnnetpe slpvmarlsq vevegddwil idravegtta
       61 leeswyvtpp acftragpvn vetspledll iehpsmsvyr ataspvapdt ppptpdapee
      121 rdveedvlpa vvsgtpaeaa slerpsprrs edeeatrrpa ihdgrpvsgr vrtekrivql
      181 rsaqkqlnyv qvlekrstqa lkrgrlersn klrevfsvkg krprrqdrlr iqnsgannnr
      241 kc
//
LOCUS       XP_034184931             242 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p53 inducible nuclear protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184931
VERSION     XP_034184931.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329040.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="tumor protein p53 inducible nuclear protein
                     isoform X1"
                     /calculated_mol_wt=26682
     Region          <61..239
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..242
                     /gene="TP53INP"
                     /coded_by="XM_034329040.2:331..1059"
                     /db_xref="GeneID:117606512"
ORIGIN      
        1 mlsslanyll ggnisgaqds regsnnetpe slpvmarlsq vevegddwil idravegtta
       61 leeswyvtpp acftragpvn vetspledll iehpsmsvyr ataspvapdt ppptpdapee
      121 rdveedvlpa vvsgtpaeaa slerpsprrs edeeatrrpa ihdgrpvsgr vrtekrivql
      181 rsaqkqlnyv qvlekrstqa lkrgrlersn klrevfsvkg krprrqdrlr iqnsgannnr
      241 kc
//
LOCUS       XP_034184932             242 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p53 inducible nuclear protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184932
VERSION     XP_034184932.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329041.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="tumor protein p53 inducible nuclear protein
                     isoform X1"
                     /calculated_mol_wt=26682
     Region          <61..239
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..242
                     /gene="TP53INP"
                     /coded_by="XM_034329041.2:167..895"
                     /db_xref="GeneID:117606512"
ORIGIN      
        1 mlsslanyll ggnisgaqds regsnnetpe slpvmarlsq vevegddwil idravegtta
       61 leeswyvtpp acftragpvn vetspledll iehpsmsvyr ataspvapdt ppptpdapee
      121 rdveedvlpa vvsgtpaeaa slerpsprrs edeeatrrpa ihdgrpvsgr vrtekrivql
      181 rsaqkqlnyv qvlekrstqa lkrgrlersn klrevfsvkg krprrqdrlr iqnsgannnr
      241 kc
//
LOCUS       XP_034184933             242 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p53 inducible nuclear protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184933
VERSION     XP_034184933.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329042.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="tumor protein p53 inducible nuclear protein
                     isoform X1"
                     /calculated_mol_wt=26682
     Region          <61..239
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..242
                     /gene="TP53INP"
                     /coded_by="XM_034329042.2:174..902"
                     /db_xref="GeneID:117606512"
ORIGIN      
        1 mlsslanyll ggnisgaqds regsnnetpe slpvmarlsq vevegddwil idravegtta
       61 leeswyvtpp acftragpvn vetspledll iehpsmsvyr ataspvapdt ppptpdapee
      121 rdveedvlpa vvsgtpaeaa slerpsprrs edeeatrrpa ihdgrpvsgr vrtekrivql
      181 rsaqkqlnyv qvlekrstqa lkrgrlersn klrevfsvkg krprrqdrlr iqnsgannnr
      241 kc
//
LOCUS       XP_034184934             242 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p53 inducible nuclear protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184934
VERSION     XP_034184934.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329043.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="tumor protein p53 inducible nuclear protein
                     isoform X1"
                     /calculated_mol_wt=26682
     Region          <61..239
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..242
                     /gene="TP53INP"
                     /coded_by="XM_034329043.2:845..1573"
                     /db_xref="GeneID:117606512"
ORIGIN      
        1 mlsslanyll ggnisgaqds regsnnetpe slpvmarlsq vevegddwil idravegtta
       61 leeswyvtpp acftragpvn vetspledll iehpsmsvyr ataspvapdt ppptpdapee
      121 rdveedvlpa vvsgtpaeaa slerpsprrs edeeatrrpa ihdgrpvsgr vrtekrivql
      181 rsaqkqlnyv qvlekrstqa lkrgrlersn klrevfsvkg krprrqdrlr iqnsgannnr
      241 kc
//
LOCUS       XP_034184927             236 aa            linear   INV 03-SEP-2025
DEFINITION  tumor protein p53 inducible nuclear protein isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184927
VERSION     XP_034184927.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329036.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="tumor protein p53 inducible nuclear protein
                     isoform X2"
                     /calculated_mol_wt=25936
     Region          <61..233
                     /region_name="DOR"
                     /note="DOR family; pfam14839"
                     /db_xref="CDD:464343"
     CDS             1..236
                     /gene="TP53INP"
                     /coded_by="XM_034329036.2:177..887"
                     /db_xref="GeneID:117606512"
ORIGIN      
        1 mlsslanyll ggnisgaqds regsnnetpe slpvmarlsq vevegddwil idravegtta
       61 leeswyvtpp acftragpvn vetspledll iehpsmsvyr ataspvapdt ppptpdapee
      121 rdveedvlpa vvsgtpaeaa slerpsprrs edeeatrrpa ihdgrpvsgr vrtekrivql
      181 rsaqkvlekr stqalkrgrl ersnklrevf svkgkrprrq drlriqnsga nnnrkc
//
LOCUS       XP_034184937             196 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606515 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184937
VERSION     XP_034184937.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329046.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="uncharacterized protein LOC117606515 isoform X1"
                     /calculated_mol_wt=22311
     CDS             1..196
                     /gene="LOC117606515"
                     /coded_by="XM_034329046.2:449..1039"
                     /db_xref="GeneID:117606515"
ORIGIN      
        1 mqskstitin nendtdesmd esvfeqicdt ifvlipevpr lkqlflswsq lgtedrvqld
       61 akvaswccpn rrqlykplqe alirwetvqd tqgapgcepg mvsfscdpql eeelvsviys
      121 lellftknrr gkeidmdyei grctdikrem slnlnsesnl rsmlspkdrt phsvhscgyi
      181 adfkksdddl sgyltn
//
LOCUS       XP_034184938             161 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606515 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184938
VERSION     XP_034184938.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329047.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="uncharacterized protein LOC117606515 isoform X2"
                     /calculated_mol_wt=18324
     CDS             1..161
                     /gene="LOC117606515"
                     /coded_by="XM_034329047.2:591..1076"
                     /db_xref="GeneID:117606515"
ORIGIN      
        1 mtvprlkqlf lswsqlgted rvqldakvas wccpnrrqly kplqealirw etvqdtqgap
       61 gcepgmvsfs cdpqleeelv sviyslellf tknrrgkeid mdyeigrctd ikremslnln
      121 sesnlrsmls pkdrtphsvh scgyiadfkk sdddlsgylt n
//
LOCUS       XP_034185049             435 aa            linear   INV 03-SEP-2025
DEFINITION  tachykinin-like peptides receptor 86C [Osmia lignaria lignaria].
ACCESSION   XP_034185049
VERSION     XP_034185049.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329158.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..435
                     /product="tachykinin-like peptides receptor 86C"
                     /calculated_mol_wt=51001
     Region          73..363
                     /region_name="7tmA_TACR"
                     /note="neurokinin receptors (or tachykinin receptors),
                     member of the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15390"
                     /db_xref="CDD:320512"
     Region          74..100
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320512"
     Region          107..133
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320512"
     Site            order(129,132..133,145..150,152..153,156,199,201..205,240,
                     243..245,247..249,251..252,308,311..312,314..315,318,
                     330..331,333..335,338,341..342)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320512"
     Region          145..175
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320512"
     Region          186..207
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320512"
     Region          240..269
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320512"
     Region          288..318
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320512"
     Region          331..356
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320512"
     CDS             1..435
                     /gene="LOC117606572"
                     /coded_by="XM_034329158.2:1..1308"
                     /db_xref="GeneID:117606572"
ORIGIN      
        1 mnslilhgly ncsaavldrn vtfllmlnrs ellsvlgdal dssserdalr daffdcifny
       61 qerpfdlpww qklswslvfa amllvatggn iiviwivlah srmrtvtnyf lvnlsmadlm
      121 msllncafnf ifllnsdwpf gvvyctinnf vahvtvassv ltlvvisfdr ymaimrplkh
      181 hmsrkrtviv vfliwtissi lavpcllyst tdsrrysngr triscyllwp dgpylhskie
      241 yfynlvflsv tylipmtima icytfmgckl wgsksigelt yyqkksmksk rkvvkmfiiv
      301 vtifaicwlp yqaffifvyh hrhftessyi qhvylsfywl amsnamvnpi iyywmnnrfr
      361 vyfqlvickw etknsgnrpm qefvnlqrsd iipynssrfk ssssrwkhem tdtqtqnfrt
      421 rssyrrshkq natvv
//
LOCUS       XP_034190523            1975 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-helicase-DNA-binding protein Mi-2 homolog isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190523
VERSION     XP_034190523.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334632.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190523.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1975
                     /product="chromodomain-helicase-DNA-binding protein Mi-2
                     homolog isoform X1"
                     /calculated_mol_wt=223196
     Region          151..204
                     /region_name="CHDNT"
                     /note="CHDNT (NUC034) domain; pfam08073"
                     /db_xref="CDD:462357"
     Region          371..413
                     /region_name="PHD1_CHD_II"
                     /note="PHD finger 1 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15531"
                     /db_xref="CDD:277006"
     Site            order(371,380..384,388,408)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277006"
     Region          431..473
                     /region_name="PHD2_CHD_II"
                     /note="PHD finger 2 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15532"
                     /db_xref="CDD:277007"
     Site            order(431,440..444,448,468)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277007"
     Region          479..549
                     /region_name="CD1_tandem_CHD3-4_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18667"
                     /db_xref="CDD:349314"
     Site            order(480,520,522..523,527)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349314"
     Region          598..650
                     /region_name="CD2_tandem_CHD3-4_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18662"
                     /db_xref="CDD:349309"
     Site            order(618,635)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349309"
     Region          726..>1222
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          1278..1329
                     /region_name="DUF1087"
                     /note="CHD subfamily II, DUF1087; pfam06465"
                     /db_xref="CDD:461922"
     Region          1393..1523
                     /region_name="CHDII_SANT-like"
                     /note="CHD subfamily II, SANT-like domain; pfam06461"
                     /db_xref="CDD:461920"
     Region          1530..>1715
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     Region          1745..1890
                     /region_name="CHDCT2"
                     /note="CHDCT2 (NUC038) domain; pfam08074"
                     /db_xref="CDD:462358"
     CDS             1..1975
                     /gene="Mi-2"
                     /coded_by="XM_034334632.2:214..6141"
                     /db_xref="GeneID:117608897"
ORIGIN      
        1 masdeevdes yageddidet ggqvsnvnqq vdgssdaees qrleedddye peerkkkkgk
       61 krkarsedkk gkkkkkkkks dsgdesdfgg ggetgdvagd dsdyagnrks rksssrksss
      121 hntpaptnqe pttgmptiee vcntfgltdv qieytdadfq nlttyklfqq hvrplltken
      181 pkvpmsklmm lvaakwrdfs elnphtqpda dvssanvded srnaranrgg gvqegedeed
      241 ddedsdrkrk srgsrakkgk kaskvptlki klgkrkrgss deeaegsgag tdrdsdmefe
      301 qmladaeepa gtdgstkgnt eengveppae ppvrrkaktk ignktkkkkk tkttskfpdg
      361 eeglqtdhqd ycevcqqgge iilcdtcpra yhlvclepel eetpegkwsc phcegegitg
      421 aaedddehme fcrickdgge llccdsctsa yhthclnppl seipdgdwkc prcscppipg
      481 kvakiltwrw kecsetpsee pstskaapkq rkireffvkw admsywhcdw itelqldvfh
      541 plmfrnysrk ydmdeppkle epldesdtrv krlkeqdgal nrddynleer fyrygvrpew
      601 lvvhrvinhr lsrdgratyl vkwrelgydq atwedehedi pglkqaieyy ldlraanccd
      661 gstsrkgkkg ekskgkkskt reliddeert pkrytpppdk pttdlkkkye rqpeyldqtg
      721 mqlhpyqleg lnwlryswgq gidtiladem glgktiqtit flyslykegh ckgpflvsvp
      781 lstiinwere fetwapdfyc vtyvgdkdsr ivirenelsf eegavrggra skirssqikf
      841 nvlltsyeli sidsaclgsi dwavlvvdea hrlksnqskf frllasynia ykllltgtpl
      901 qnnleelfhl lnflcrdkfn dlaafqnefa diskeeqvkk lhellgphml rrlkadvlkn
      961 mpsksefivr velspmqkky ykyiltrnfe alnpkgggqq vsllnimmdl kkccnhpylf
     1021 paasqeapta pngtyetsal ikaagklvll skmlkklrdd ghrvlifsqm tkmldiledy
     1081 legegykyer idgnitgaqr qeaidrfnap gaqqfvflls tragglginl atadtviiyd
     1141 sdwnphndiq afsrahrigq ankvmiyrfv trnsveervt qvakrkmmlt hlvvrpgmgg
     1201 kganfskqel ddilrfgtee lfkeeegked eaihyddkav aelldrskeg ieqkenwane
     1261 ylssfkvasy vtkegeteee adteiikqea entdpaywik llrhhyeqqq ediartlgkg
     1321 krirkqvnyn dgvvtgdqst rddqpwqenl sdynsdfsap sdddkedddf dekgdgdlls
     1381 rrsrrrlerr dekdrplppl larvngniev lgfnarqrka flnaimrygm ppqdafnsqc
     1441 ilshfrlvrd lrgkseknfk ayvslfmrhl cepgadnaea fadgvpregl srqhvltrig
     1501 vmslirkkvq efehingyys mpemirkpve pvkvepgaea atgtsstsat patsnapsps
     1561 paatptpltv pgtastetsk vnsdaseake ckeepkdkds vdgkdvkeep kdskeeednn
     1621 aekdkekedv kkeekdadae gtdkekdakd vkdeksaaks dekvessdnk pkqdseedvv
     1681 ivkddeeete kredkdskdk dvkdcdseil kpkrkfmfni adggftelht lwlneekaav
     1741 pgreyeiwhr rhdywllagi vthgygrwqd iqndirfaii nepfkmdvgk gnfleiknkf
     1801 larrfklleq alvieeqlrr aaylnltqdp nhpamslnar faeveclaes hqhlskesla
     1861 gnkpanavlh kvlnqleell sdmksdvsrl patlarippv aqrlqmsers ilsrlaatap
     1921 ggsssqsgqa allaqqfpag fsggqlpatf agaanfgnfr pqysvpgqpp qgfta
//
LOCUS       XP_034190524            1972 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-helicase-DNA-binding protein Mi-2 homolog isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190524
VERSION     XP_034190524.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334633.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190524.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1972
                     /product="chromodomain-helicase-DNA-binding protein Mi-2
                     homolog isoform X2"
                     /calculated_mol_wt=222851
     Region          151..204
                     /region_name="CHDNT"
                     /note="CHDNT (NUC034) domain; pfam08073"
                     /db_xref="CDD:462357"
     Region          371..413
                     /region_name="PHD1_CHD_II"
                     /note="PHD finger 1 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15531"
                     /db_xref="CDD:277006"
     Site            order(371,380..384,388,408)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277006"
     Region          431..473
                     /region_name="PHD2_CHD_II"
                     /note="PHD finger 2 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15532"
                     /db_xref="CDD:277007"
     Site            order(431,440..444,448,468)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277007"
     Region          479..549
                     /region_name="CD1_tandem_CHD3-4_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18667"
                     /db_xref="CDD:349314"
     Site            order(480,520,522..523,527)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349314"
     Region          598..650
                     /region_name="CD2_tandem_CHD3-4_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18662"
                     /db_xref="CDD:349309"
     Site            order(618,635)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349309"
     Region          723..>1219
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          1275..1326
                     /region_name="DUF1087"
                     /note="CHD subfamily II, DUF1087; pfam06465"
                     /db_xref="CDD:461922"
     Region          1390..1520
                     /region_name="CHDII_SANT-like"
                     /note="CHD subfamily II, SANT-like domain; pfam06461"
                     /db_xref="CDD:461920"
     Region          1527..>1712
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     Region          1742..1887
                     /region_name="CHDCT2"
                     /note="CHDCT2 (NUC038) domain; pfam08074"
                     /db_xref="CDD:462358"
     CDS             1..1972
                     /gene="Mi-2"
                     /coded_by="XM_034334633.2:213..6131"
                     /db_xref="GeneID:117608897"
ORIGIN      
        1 masdeevdes yageddidet ggqvsnvnqq vdgssdaees qrleedddye peerkkkkgk
       61 krkarsedkk gkkkkkkkks dsgdesdfgg ggetgdvagd dsdyagnrks rksssrksss
      121 hntpaptnqe pttgmptiee vcntfgltdv qieytdadfq nlttyklfqq hvrplltken
      181 pkvpmsklmm lvaakwrdfs elnphtqpda dvssanvded srnaranrgg gvqegedeed
      241 ddedsdrkrk srgsrakkgk kaskvptlki klgkrkrgss deeaegsgag tdrdsdmefe
      301 qmladaeepa gtdgstkgnt eengveppae ppvrrkaktk ignktkkkkk tkttskfpdg
      361 eeglqtdhqd ycevcqqgge iilcdtcpra yhlvclepel eetpegkwsc phcegegitg
      421 aaedddehme fcrickdgge llccdsctsa yhthclnppl seipdgdwkc prcscppipg
      481 kvakiltwrw kecsetpsee pstskaapkq rkireffvkw admsywhcdw itelqldvfh
      541 plmfrnysrk ydmdeppkle epldesdtrv krlkeqdgal nrddynleer fyrygvrpew
      601 lvvhrvinhr lsrdgratyl vkwrelgydq atwedehedi pglkqaieyy ldlraanccd
      661 gstsrkgkkg kgkksktrel iddeertpkr ytpppdkptt dlkkkyerqp eyldqtgmql
      721 hpyqleglnw lryswgqgid tilademglg ktiqtitfly slykeghckg pflvsvplst
      781 iinwerefet wapdfycvty vgdkdsrivi renelsfeeg avrggraski rssqikfnvl
      841 ltsyelisid saclgsidwa vlvvdeahrl ksnqskffrl lasyniaykl lltgtplqnn
      901 leelfhllnf lcrdkfndla afqnefadis keeqvkklhe llgphmlrrl kadvlknmps
      961 ksefivrvel spmqkkyyky iltrnfealn pkgggqqvsl lnimmdlkkc cnhpylfpaa
     1021 sqeaptapng tyetsalika agklvllskm lkklrddghr vlifsqmtkm ldiledyleg
     1081 egykyeridg nitgaqrqea idrfnapgaq qfvfllstra gglginlata dtviiydsdw
     1141 nphndiqafs rahrigqank vmiyrfvtrn sveervtqva krkmmlthlv vrpgmggkga
     1201 nfskqelddi lrfgteelfk eeegkedeai hyddkavael ldrskegieq kenwaneyls
     1261 sfkvasyvtk egeteeeadt eiikqeaent dpaywikllr hhyeqqqedi artlgkgkri
     1321 rkqvnyndgv vtgdqstrdd qpwqenlsdy nsdfsapsdd dkedddfdek gdgdllsrrs
     1381 rrrlerrdek drplppllar vngnievlgf narqrkafln aimrygmppq dafnsqcils
     1441 hfrlvrdlrg kseknfkayv slfmrhlcep gadnaeafad gvpreglsrq hvltrigvms
     1501 lirkkvqefe hingyysmpe mirkpvepvk vepgaeaatg tsstsatpat snapspspaa
     1561 tptpltvpgt astetskvns daseakecke epkdkdsvdg kdvkeepkds keeednnaek
     1621 dkekedvkke ekdadaegtd kekdakdvkd eksaaksdek vessdnkpkq dseedvvivk
     1681 ddeeetekre dkdskdkdvk dcdseilkpk rkfmfniadg gftelhtlwl neekaavpgr
     1741 eyeiwhrrhd ywllagivth gygrwqdiqn dirfaiinep fkmdvgkgnf leiknkflar
     1801 rfklleqalv ieeqlrraay lnltqdpnhp amslnarfae veclaeshqh lskeslagnk
     1861 panavlhkvl nqleellsdm ksdvsrlpat larippvaqr lqmsersils rlaatapggs
     1921 ssqsgqaall aqqfpagfsg gqlpatfaga anfgnfrpqy svpgqppqgf ta
//
LOCUS       XP_034190525            1972 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-helicase-DNA-binding protein Mi-2 homolog isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034190525
VERSION     XP_034190525.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334634.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190525.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1972
                     /product="chromodomain-helicase-DNA-binding protein Mi-2
                     homolog isoform X3"
                     /calculated_mol_wt=222924
     Region          151..204
                     /region_name="CHDNT"
                     /note="CHDNT (NUC034) domain; pfam08073"
                     /db_xref="CDD:462357"
     Region          371..413
                     /region_name="PHD1_CHD_II"
                     /note="PHD finger 1 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15531"
                     /db_xref="CDD:277006"
     Site            order(371,380..384,388,408)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277006"
     Region          428..470
                     /region_name="PHD2_CHD_II"
                     /note="PHD finger 2 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15532"
                     /db_xref="CDD:277007"
     Site            order(428,437..441,445,465)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277007"
     Region          476..546
                     /region_name="CD1_tandem_CHD3-4_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18667"
                     /db_xref="CDD:349314"
     Site            order(477,517,519..520,524)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349314"
     Region          595..647
                     /region_name="CD2_tandem_CHD3-4_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18662"
                     /db_xref="CDD:349309"
     Site            order(615,632)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349309"
     Region          723..>1219
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          1275..1326
                     /region_name="DUF1087"
                     /note="CHD subfamily II, DUF1087; pfam06465"
                     /db_xref="CDD:461922"
     Region          1390..1520
                     /region_name="CHDII_SANT-like"
                     /note="CHD subfamily II, SANT-like domain; pfam06461"
                     /db_xref="CDD:461920"
     Region          1527..>1712
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     Region          1742..1887
                     /region_name="CHDCT2"
                     /note="CHDCT2 (NUC038) domain; pfam08074"
                     /db_xref="CDD:462358"
     CDS             1..1972
                     /gene="Mi-2"
                     /coded_by="XM_034334634.2:214..6132"
                     /db_xref="GeneID:117608897"
ORIGIN      
        1 masdeevdes yageddidet ggqvsnvnqq vdgssdaees qrleedddye peerkkkkgk
       61 krkarsedkk gkkkkkkkks dsgdesdfgg ggetgdvagd dsdyagnrks rksssrksss
      121 hntpaptnqe pttgmptiee vcntfgltdv qieytdadfq nlttyklfqq hvrplltken
      181 pkvpmsklmm lvaakwrdfs elnphtqpda dvssanvded srnaranrgg gvqegedeed
      241 ddedsdrkrk srgsrakkgk kaskvptlki klgkrkrgss deeaegsgag tdrdsdmefe
      301 qmladaeepa gtdgstkgnt eengveppae ppvrrkaktk ignktkkkkk tkttskfpdg
      361 eeglqtdhqd ycevcqqgge iilcdtcpra yhlvclepel eetpegkwsc phcegegaae
      421 dddehmefcr ickdggellc cdsctsayht hclnpplsei pdgdwkcprc scppipgkva
      481 kiltwrwkec setpseepst skaapkqrki reffvkwadm sywhcdwite lqldvfhplm
      541 frnysrkydm deppkleepl desdtrvkrl keqdgalnrd dynleerfyr ygvrpewlvv
      601 hrvinhrlsr dgratylvkw relgydqatw edehedipgl kqaieyyldl raanccdgst
      661 srkgkkgeks kgkksktrel iddeertpkr ytpppdkptt dlkkkyerqp eyldqtgmql
      721 hpyqleglnw lryswgqgid tilademglg ktiqtitfly slykeghckg pflvsvplst
      781 iinwerefet wapdfycvty vgdkdsrivi renelsfeeg avrggraski rssqikfnvl
      841 ltsyelisid saclgsidwa vlvvdeahrl ksnqskffrl lasyniaykl lltgtplqnn
      901 leelfhllnf lcrdkfndla afqnefadis keeqvkklhe llgphmlrrl kadvlknmps
      961 ksefivrvel spmqkkyyky iltrnfealn pkgggqqvsl lnimmdlkkc cnhpylfpaa
     1021 sqeaptapng tyetsalika agklvllskm lkklrddghr vlifsqmtkm ldiledyleg
     1081 egykyeridg nitgaqrqea idrfnapgaq qfvfllstra gglginlata dtviiydsdw
     1141 nphndiqafs rahrigqank vmiyrfvtrn sveervtqva krkmmlthlv vrpgmggkga
     1201 nfskqelddi lrfgteelfk eeegkedeai hyddkavael ldrskegieq kenwaneyls
     1261 sfkvasyvtk egeteeeadt eiikqeaent dpaywikllr hhyeqqqedi artlgkgkri
     1321 rkqvnyndgv vtgdqstrdd qpwqenlsdy nsdfsapsdd dkedddfdek gdgdllsrrs
     1381 rrrlerrdek drplppllar vngnievlgf narqrkafln aimrygmppq dafnsqcils
     1441 hfrlvrdlrg kseknfkayv slfmrhlcep gadnaeafad gvpreglsrq hvltrigvms
     1501 lirkkvqefe hingyysmpe mirkpvepvk vepgaeaatg tsstsatpat snapspspaa
     1561 tptpltvpgt astetskvns daseakecke epkdkdsvdg kdvkeepkds keeednnaek
     1621 dkekedvkke ekdadaegtd kekdakdvkd eksaaksdek vessdnkpkq dseedvvivk
     1681 ddeeetekre dkdskdkdvk dcdseilkpk rkfmfniadg gftelhtlwl neekaavpgr
     1741 eyeiwhrrhd ywllagivth gygrwqdiqn dirfaiinep fkmdvgkgnf leiknkflar
     1801 rfklleqalv ieeqlrraay lnltqdpnhp amslnarfae veclaeshqh lskeslagnk
     1861 panavlhkvl nqleellsdm ksdvsrlpat larippvaqr lqmsersils rlaatapggs
     1921 ssqsgqaall aqqfpagfsg gqlpatfaga anfgnfrpqy svpgqppqgf ta
//
LOCUS       XP_034190526            1969 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-helicase-DNA-binding protein Mi-2 homolog isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034190526
VERSION     XP_034190526.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334635.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190526.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1969
                     /product="chromodomain-helicase-DNA-binding protein Mi-2
                     homolog isoform X4"
                     /calculated_mol_wt=222525
     Region          151..204
                     /region_name="CHDNT"
                     /note="CHDNT (NUC034) domain; pfam08073"
                     /db_xref="CDD:462357"
     Region          371..413
                     /region_name="PHD1_CHD_II"
                     /note="PHD finger 1 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15531"
                     /db_xref="CDD:277006"
     Site            order(371,380..384,388,408)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277006"
     Region          431..473
                     /region_name="PHD2_CHD_II"
                     /note="PHD finger 2 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15532"
                     /db_xref="CDD:277007"
     Site            order(431,440..444,448,468)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277007"
     Region          479..549
                     /region_name="CD1_tandem_CHD3-4_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18667"
                     /db_xref="CDD:349314"
     Site            order(480,520,522..523,527)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349314"
     Region          598..650
                     /region_name="CD2_tandem_CHD3-4_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18662"
                     /db_xref="CDD:349309"
     Site            order(618,635)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349309"
     Region          726..>1222
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          1278..1329
                     /region_name="DUF1087"
                     /note="CHD subfamily II, DUF1087; pfam06465"
                     /db_xref="CDD:461922"
     Region          1393..1517
                     /region_name="CHDII_SANT-like"
                     /note="CHD subfamily II, SANT-like domain; pfam06461"
                     /db_xref="CDD:461920"
     Region          1524..>1709
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     Region          1739..1884
                     /region_name="CHDCT2"
                     /note="CHDCT2 (NUC038) domain; pfam08074"
                     /db_xref="CDD:462358"
     CDS             1..1969
                     /gene="Mi-2"
                     /coded_by="XM_034334635.2:213..6122"
                     /db_xref="GeneID:117608897"
ORIGIN      
        1 masdeevdes yageddidet ggqvsnvnqq vdgssdaees qrleedddye peerkkkkgk
       61 krkarsedkk gkkkkkkkks dsgdesdfgg ggetgdvagd dsdyagnrks rksssrksss
      121 hntpaptnqe pttgmptiee vcntfgltdv qieytdadfq nlttyklfqq hvrplltken
      181 pkvpmsklmm lvaakwrdfs elnphtqpda dvssanvded srnaranrgg gvqegedeed
      241 ddedsdrkrk srgsrakkgk kaskvptlki klgkrkrgss deeaegsgag tdrdsdmefe
      301 qmladaeepa gtdgstkgnt eengveppae ppvrrkaktk ignktkkkkk tkttskfpdg
      361 eeglqtdhqd ycevcqqgge iilcdtcpra yhlvclepel eetpegkwsc phcegegitg
      421 aaedddehme fcrickdgge llccdsctsa yhthclnppl seipdgdwkc prcscppipg
      481 kvakiltwrw kecsetpsee pstskaapkq rkireffvkw admsywhcdw itelqldvfh
      541 plmfrnysrk ydmdeppkle epldesdtrv krlkeqdgal nrddynleer fyrygvrpew
      601 lvvhrvinhr lsrdgratyl vkwrelgydq atwedehedi pglkqaieyy ldlraanccd
      661 gstsrkgkkg ekskgkkskt reliddeert pkrytpppdk pttdlkkkye rqpeyldqtg
      721 mqlhpyqleg lnwlryswgq gidtiladem glgktiqtit flyslykegh ckgpflvsvp
      781 lstiinwere fetwapdfyc vtyvgdkdsr ivirenelsf eegavrggra skirssqikf
      841 nvlltsyeli sidsaclgsi dwavlvvdea hrlksnqskf frllasynia ykllltgtpl
      901 qnnleelfhl lnflcrdkfn dlaafqnefa diskeeqvkk lhellgphml rrlkadvlkn
      961 mpsksefivr velspmqkky ykyiltrnfe alnpkgggqq vsllnimmdl kkccnhpylf
     1021 paasqeapta pngtyetsal ikaagklvll skmlkklrdd ghrvlifsqm tkmldiledy
     1081 legegykyer idgnitgaqr qeaidrfnap gaqqfvflls tragglginl atadtviiyd
     1141 sdwnphndiq afsrahrigq ankvmiyrfv trnsveervt qvakrkmmlt hlvvrpgmgg
     1201 kganfskqel ddilrfgtee lfkeeegked eaihyddkav aelldrskeg ieqkenwane
     1261 ylssfkvasy vtkegeteee adteiikqea entdpaywik llrhhyeqqq ediartlgkg
     1321 krirkqvnyn dgvvtgdqst rddqpwqenl sdynsdfsap sdddkedddf dekgdgdlls
     1381 rrsrrrlerr dekdrplppl larvngniev lgfnarqrka flnaimrygm ppqdafnsqw
     1441 lvrdlrgkse knfkayvslf mrhlcepgad naeafadgvp reglsrqhvl trigvmslir
     1501 kkvqefehin gyysmpemir kpvepvkvep gaeaatgtss tsatpatsna pspspaatpt
     1561 pltvpgtast etskvnsdas eakeckeepk dkdsvdgkdv keepkdskee ednnaekdke
     1621 kedvkkeekd adaegtdkek dakdvkdeks aaksdekves sdnkpkqdse edvvivkdde
     1681 eetekredkd skdkdvkdcd seilkpkrkf mfniadggft elhtlwlnee kaavpgreye
     1741 iwhrrhdywl lagivthgyg rwqdiqndir faiinepfkm dvgkgnflei knkflarrfk
     1801 lleqalviee qlrraaylnl tqdpnhpams lnarfaevec laeshqhlsk eslagnkpan
     1861 avlhkvlnql eellsdmksd vsrlpatlar ippvaqrlqm sersilsrla atapggsssq
     1921 sgqaallaqq fpagfsggql patfagaanf gnfrpqysvp gqppqgfta
//
LOCUS       XP_034190527            1966 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-helicase-DNA-binding protein Mi-2 homolog isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034190527
VERSION     XP_034190527.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334636.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190527.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1966
                     /product="chromodomain-helicase-DNA-binding protein Mi-2
                     homolog isoform X5"
                     /calculated_mol_wt=222181
     Region          151..204
                     /region_name="CHDNT"
                     /note="CHDNT (NUC034) domain; pfam08073"
                     /db_xref="CDD:462357"
     Region          371..413
                     /region_name="PHD1_CHD_II"
                     /note="PHD finger 1 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15531"
                     /db_xref="CDD:277006"
     Site            order(371,380..384,388,408)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277006"
     Region          431..473
                     /region_name="PHD2_CHD_II"
                     /note="PHD finger 2 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15532"
                     /db_xref="CDD:277007"
     Site            order(431,440..444,448,468)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277007"
     Region          479..549
                     /region_name="CD1_tandem_CHD3-4_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18667"
                     /db_xref="CDD:349314"
     Site            order(480,520,522..523,527)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349314"
     Region          598..650
                     /region_name="CD2_tandem_CHD3-4_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18662"
                     /db_xref="CDD:349309"
     Site            order(618,635)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349309"
     Region          723..>1219
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          1275..1326
                     /region_name="DUF1087"
                     /note="CHD subfamily II, DUF1087; pfam06465"
                     /db_xref="CDD:461922"
     Region          1390..1514
                     /region_name="CHDII_SANT-like"
                     /note="CHD subfamily II, SANT-like domain; pfam06461"
                     /db_xref="CDD:461920"
     Region          1521..>1706
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     Region          1736..1881
                     /region_name="CHDCT2"
                     /note="CHDCT2 (NUC038) domain; pfam08074"
                     /db_xref="CDD:462358"
     CDS             1..1966
                     /gene="Mi-2"
                     /coded_by="XM_034334636.2:213..6113"
                     /db_xref="GeneID:117608897"
ORIGIN      
        1 masdeevdes yageddidet ggqvsnvnqq vdgssdaees qrleedddye peerkkkkgk
       61 krkarsedkk gkkkkkkkks dsgdesdfgg ggetgdvagd dsdyagnrks rksssrksss
      121 hntpaptnqe pttgmptiee vcntfgltdv qieytdadfq nlttyklfqq hvrplltken
      181 pkvpmsklmm lvaakwrdfs elnphtqpda dvssanvded srnaranrgg gvqegedeed
      241 ddedsdrkrk srgsrakkgk kaskvptlki klgkrkrgss deeaegsgag tdrdsdmefe
      301 qmladaeepa gtdgstkgnt eengveppae ppvrrkaktk ignktkkkkk tkttskfpdg
      361 eeglqtdhqd ycevcqqgge iilcdtcpra yhlvclepel eetpegkwsc phcegegitg
      421 aaedddehme fcrickdgge llccdsctsa yhthclnppl seipdgdwkc prcscppipg
      481 kvakiltwrw kecsetpsee pstskaapkq rkireffvkw admsywhcdw itelqldvfh
      541 plmfrnysrk ydmdeppkle epldesdtrv krlkeqdgal nrddynleer fyrygvrpew
      601 lvvhrvinhr lsrdgratyl vkwrelgydq atwedehedi pglkqaieyy ldlraanccd
      661 gstsrkgkkg kgkksktrel iddeertpkr ytpppdkptt dlkkkyerqp eyldqtgmql
      721 hpyqleglnw lryswgqgid tilademglg ktiqtitfly slykeghckg pflvsvplst
      781 iinwerefet wapdfycvty vgdkdsrivi renelsfeeg avrggraski rssqikfnvl
      841 ltsyelisid saclgsidwa vlvvdeahrl ksnqskffrl lasyniaykl lltgtplqnn
      901 leelfhllnf lcrdkfndla afqnefadis keeqvkklhe llgphmlrrl kadvlknmps
      961 ksefivrvel spmqkkyyky iltrnfealn pkgggqqvsl lnimmdlkkc cnhpylfpaa
     1021 sqeaptapng tyetsalika agklvllskm lkklrddghr vlifsqmtkm ldiledyleg
     1081 egykyeridg nitgaqrqea idrfnapgaq qfvfllstra gglginlata dtviiydsdw
     1141 nphndiqafs rahrigqank vmiyrfvtrn sveervtqva krkmmlthlv vrpgmggkga
     1201 nfskqelddi lrfgteelfk eeegkedeai hyddkavael ldrskegieq kenwaneyls
     1261 sfkvasyvtk egeteeeadt eiikqeaent dpaywikllr hhyeqqqedi artlgkgkri
     1321 rkqvnyndgv vtgdqstrdd qpwqenlsdy nsdfsapsdd dkedddfdek gdgdllsrrs
     1381 rrrlerrdek drplppllar vngnievlgf narqrkafln aimrygmppq dafnsqwlvr
     1441 dlrgkseknf kayvslfmrh lcepgadnae afadgvpreg lsrqhvltri gvmslirkkv
     1501 qefehingyy smpemirkpv epvkvepgae aatgtsstsa tpatsnapsp spaatptplt
     1561 vpgtastets kvnsdaseak eckeepkdkd svdgkdvkee pkdskeeedn naekdkeked
     1621 vkkeekdada egtdkekdak dvkdeksaak sdekvessdn kpkqdseedv vivkddeeet
     1681 ekredkdskd kdvkdcdsei lkpkrkfmfn iadggftelh tlwlneekaa vpgreyeiwh
     1741 rrhdywllag ivthgygrwq diqndirfai inepfkmdvg kgnfleiknk flarrfklle
     1801 qalvieeqlr raaylnltqd pnhpamslna rfaeveclae shqhlskesl agnkpanavl
     1861 hkvlnqleel lsdmksdvsr lpatlaripp vaqrlqmser silsrlaata pggsssqsgq
     1921 aallaqqfpa gfsggqlpat fagaanfgnf rpqysvpgqp pqgfta
//
LOCUS       XP_034190528            1964 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-helicase-DNA-binding protein Mi-2 homolog isoform X6
            [Osmia lignaria lignaria].
ACCESSION   XP_034190528
VERSION     XP_034190528.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334637.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190528.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1964
                     /product="chromodomain-helicase-DNA-binding protein Mi-2
                     homolog isoform X6"
                     /calculated_mol_wt=222000
     Region          140..193
                     /region_name="CHDNT"
                     /note="CHDNT (NUC034) domain; pfam08073"
                     /db_xref="CDD:462357"
     Region          360..402
                     /region_name="PHD1_CHD_II"
                     /note="PHD finger 1 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15531"
                     /db_xref="CDD:277006"
     Site            order(360,369..373,377,397)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277006"
     Region          420..462
                     /region_name="PHD2_CHD_II"
                     /note="PHD finger 2 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15532"
                     /db_xref="CDD:277007"
     Site            order(420,429..433,437,457)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277007"
     Region          468..538
                     /region_name="CD1_tandem_CHD3-4_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18667"
                     /db_xref="CDD:349314"
     Site            order(469,509,511..512,516)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349314"
     Region          587..639
                     /region_name="CD2_tandem_CHD3-4_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18662"
                     /db_xref="CDD:349309"
     Site            order(607,624)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349309"
     Region          715..>1211
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          1267..1318
                     /region_name="DUF1087"
                     /note="CHD subfamily II, DUF1087; pfam06465"
                     /db_xref="CDD:461922"
     Region          1382..1512
                     /region_name="CHDII_SANT-like"
                     /note="CHD subfamily II, SANT-like domain; pfam06461"
                     /db_xref="CDD:461920"
     Region          1519..>1704
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     Region          1734..1879
                     /region_name="CHDCT2"
                     /note="CHDCT2 (NUC038) domain; pfam08074"
                     /db_xref="CDD:462358"
     CDS             1..1964
                     /gene="Mi-2"
                     /coded_by="XM_034334637.2:122..6016"
                     /db_xref="GeneID:117608897"
ORIGIN      
        1 mgeddidetg gqvsnvnqqv dgssdaeesq rleedddyep eerkkkkgkk rkarsedkkg
       61 kkkkkkkksd sgdesdfggg getgdvagdd sdyagnrksr ksssrksssh ntpaptnqep
      121 ttgmptieev cntfgltdvq ieytdadfqn lttyklfqqh vrplltkenp kvpmsklmml
      181 vaakwrdfse lnphtqpdad vssanvdeds rnaranrggg vqegedeedd dedsdrkrks
      241 rgsrakkgkk askvptlkik lgkrkrgssd eeaegsgagt drdsdmefeq mladaeepag
      301 tdgstkgnte engveppaep pvrrkaktki gnktkkkkkt kttskfpdge eglqtdhqdy
      361 cevcqqggei ilcdtcpray hlvclepele etpegkwscp hcegegitga aedddehmef
      421 crickdggel lccdsctsay hthclnppls eipdgdwkcp rcscppipgk vakiltwrwk
      481 ecsetpseep stskaapkqr kireffvkwa dmsywhcdwi telqldvfhp lmfrnysrky
      541 dmdeppklee pldesdtrvk rlkeqdgaln rddynleerf yrygvrpewl vvhrvinhrl
      601 srdgratylv kwrelgydqa twedehedip glkqaieyyl dlraanccdg stsrkgkkge
      661 kskgkksktr eliddeertp krytpppdkp ttdlkkkyer qpeyldqtgm qlhpyqlegl
      721 nwlryswgqg idtilademg lgktiqtitf lyslykeghc kgpflvsvpl stiinweref
      781 etwapdfycv tyvgdkdsri virenelsfe egavrggras kirssqikfn vlltsyelis
      841 idsaclgsid wavlvvdeah rlksnqskff rllasyniay kllltgtplq nnleelfhll
      901 nflcrdkfnd laafqnefad iskeeqvkkl hellgphmlr rlkadvlknm psksefivrv
      961 elspmqkkyy kyiltrnfea lnpkgggqqv sllnimmdlk kccnhpylfp aasqeaptap
     1021 ngtyetsali kaagklvlls kmlkklrddg hrvlifsqmt kmldiledyl egegykyeri
     1081 dgnitgaqrq eaidrfnapg aqqfvfllst ragglginla tadtviiyds dwnphndiqa
     1141 fsrahrigqa nkvmiyrfvt rnsveervtq vakrkmmlth lvvrpgmggk ganfskqeld
     1201 dilrfgteel fkeeegkede aihyddkava elldrskegi eqkenwaney lssfkvasyv
     1261 tkegeteeea dteiikqeae ntdpaywikl lrhhyeqqqe diartlgkgk rirkqvnynd
     1321 gvvtgdqstr ddqpwqenls dynsdfsaps dddkedddfd ekgdgdllsr rsrrrlerrd
     1381 ekdrplppll arvngnievl gfnarqrkaf lnaimrygmp pqdafnsqci lshfrlvrdl
     1441 rgkseknfka yvslfmrhlc epgadnaeaf adgvpregls rqhvltrigv mslirkkvqe
     1501 fehingyysm pemirkpvep vkvepgaeaa tgtsstsatp atsnapspsp aatptpltvp
     1561 gtastetskv nsdaseakec keepkdkdsv dgkdvkeepk dskeeednna ekdkekedvk
     1621 keekdadaeg tdkekdakdv kdeksaaksd ekvessdnkp kqdseedvvi vkddeeetek
     1681 redkdskdkd vkdcdseilk pkrkfmfnia dggftelhtl wlneekaavp greyeiwhrr
     1741 hdywllagiv thgygrwqdi qndirfaiin epfkmdvgkg nfleiknkfl arrfklleqa
     1801 lvieeqlrra aylnltqdpn hpamslnarf aeveclaesh qhlskeslag nkpanavlhk
     1861 vlnqleells dmksdvsrlp atlarippva qrlqmsersi lsrlaatapg gsssqsgqaa
     1921 llaqqfpagf sggqlpatfa gaanfgnfrp qysvpgqppq gfta
//
LOCUS       XP_034190529            1963 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-helicase-DNA-binding protein Mi-2 homolog isoform X7
            [Osmia lignaria lignaria].
ACCESSION   XP_034190529
VERSION     XP_034190529.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334638.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190529.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1963
                     /product="chromodomain-helicase-DNA-binding protein Mi-2
                     homolog isoform X7"
                     /calculated_mol_wt=221909
     Region          151..204
                     /region_name="CHDNT"
                     /note="CHDNT (NUC034) domain; pfam08073"
                     /db_xref="CDD:462357"
     Region          371..413
                     /region_name="PHD1_CHD_II"
                     /note="PHD finger 1 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15531"
                     /db_xref="CDD:277006"
     Site            order(371,380..384,388,408)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277006"
     Region          428..470
                     /region_name="PHD2_CHD_II"
                     /note="PHD finger 2 found in class II
                     Chromodomain-Helicase-DNA binding (CHD) proteins; cd15532"
                     /db_xref="CDD:277007"
     Site            order(428,437..441,445,465)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277007"
     Region          476..546
                     /region_name="CD1_tandem_CHD3-4_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18667"
                     /db_xref="CDD:349314"
     Site            order(477,517,519..520,524)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349314"
     Region          595..647
                     /region_name="CD2_tandem_CHD3-4_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 3 and 4, and
                     similar proteins; cd18662"
                     /db_xref="CDD:349309"
     Site            order(615,632)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349309"
     Region          720..>1216
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          1272..1323
                     /region_name="DUF1087"
                     /note="CHD subfamily II, DUF1087; pfam06465"
                     /db_xref="CDD:461922"
     Region          1387..1511
                     /region_name="CHDII_SANT-like"
                     /note="CHD subfamily II, SANT-like domain; pfam06461"
                     /db_xref="CDD:461920"
     Region          1518..>1703
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     Region          1733..1878
                     /region_name="CHDCT2"
                     /note="CHDCT2 (NUC038) domain; pfam08074"
                     /db_xref="CDD:462358"
     CDS             1..1963
                     /gene="Mi-2"
                     /coded_by="XM_034334638.2:213..6104"
                     /db_xref="GeneID:117608897"
ORIGIN      
        1 masdeevdes yageddidet ggqvsnvnqq vdgssdaees qrleedddye peerkkkkgk
       61 krkarsedkk gkkkkkkkks dsgdesdfgg ggetgdvagd dsdyagnrks rksssrksss
      121 hntpaptnqe pttgmptiee vcntfgltdv qieytdadfq nlttyklfqq hvrplltken
      181 pkvpmsklmm lvaakwrdfs elnphtqpda dvssanvded srnaranrgg gvqegedeed
      241 ddedsdrkrk srgsrakkgk kaskvptlki klgkrkrgss deeaegsgag tdrdsdmefe
      301 qmladaeepa gtdgstkgnt eengveppae ppvrrkaktk ignktkkkkk tkttskfpdg
      361 eeglqtdhqd ycevcqqgge iilcdtcpra yhlvclepel eetpegkwsc phcegegaae
      421 dddehmefcr ickdggellc cdsctsayht hclnpplsei pdgdwkcprc scppipgkva
      481 kiltwrwkec setpseepst skaapkqrki reffvkwadm sywhcdwite lqldvfhplm
      541 frnysrkydm deppkleepl desdtrvkrl keqdgalnrd dynleerfyr ygvrpewlvv
      601 hrvinhrlsr dgratylvkw relgydqatw edehedipgl kqaieyyldl raanccdgst
      661 srkgkkgkgk ksktrelidd eertpkrytp ppdkpttdlk kkyerqpeyl dqtgmqlhpy
      721 qleglnwlry swgqgidtil ademglgkti qtitflysly keghckgpfl vsvplstiin
      781 werefetwap dfycvtyvgd kdsriviren elsfeegavr ggraskirss qikfnvllts
      841 yelisidsac lgsidwavlv vdeahrlksn qskffrllas yniayklllt gtplqnnlee
      901 lfhllnflcr dkfndlaafq nefadiskee qvkklhellg phmlrrlkad vlknmpskse
      961 fivrvelspm qkkyykyilt rnfealnpkg ggqqvsllni mmdlkkccnh pylfpaasqe
     1021 aptapngtye tsalikaagk lvllskmlkk lrddghrvli fsqmtkmldi ledylegegy
     1081 kyeridgnit gaqrqeaidr fnapgaqqfv fllstraggl ginlatadtv iiydsdwnph
     1141 ndiqafsrah rigqankvmi yrfvtrnsve ervtqvakrk mmlthlvvrp gmggkganfs
     1201 kqelddilrf gteelfkeee gkedeaihyd dkavaelldr skegieqken waneylssfk
     1261 vasyvtkege teeeadteii kqeaentdpa ywikllrhhy eqqqediart lgkgkrirkq
     1321 vnyndgvvtg dqstrddqpw qenlsdynsd fsapsdddke dddfdekgdg dllsrrsrrr
     1381 lerrdekdrp lppllarvng nievlgfnar qrkaflnaim rygmppqdaf nsqwlvrdlr
     1441 gkseknfkay vslfmrhlce pgadnaeafa dgvpreglsr qhvltrigvm slirkkvqef
     1501 ehingyysmp emirkpvepv kvepgaeaat gtsstsatpa tsnapspspa atptpltvpg
     1561 tastetskvn sdaseakeck eepkdkdsvd gkdvkeepkd skeeednnae kdkekedvkk
     1621 eekdadaegt dkekdakdvk deksaaksde kvessdnkpk qdseedvviv kddeeetekr
     1681 edkdskdkdv kdcdseilkp krkfmfniad ggftelhtlw lneekaavpg reyeiwhrrh
     1741 dywllagivt hgygrwqdiq ndirfaiine pfkmdvgkgn fleiknkfla rrfklleqal
     1801 vieeqlrraa ylnltqdpnh pamslnarfa eveclaeshq hlskeslagn kpanavlhkv
     1861 lnqleellsd mksdvsrlpa tlarippvaq rlqmsersil srlaatapgg sssqsgqaal
     1921 laqqfpagfs ggqlpatfag aanfgnfrpq ysvpgqppqg fta
//
LOCUS       XP_034190530             844 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608898 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190530
VERSION     XP_034190530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334639.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="uncharacterized protein LOC117608898 isoform X1"
                     /calculated_mol_wt=92587
     Region          151..>327
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          <421..692
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          693..715
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(700,702,704,706..707,710..711,714,730,732,736..737,
                     740..741,744,758,760,762,764..765,768..769,772)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          707..734
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          723..745
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          737..760
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          751..773
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          753..773
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..844
                     /gene="LOC117608898"
                     /coded_by="XM_034334639.2:996..3530"
                     /db_xref="GeneID:117608898"
ORIGIN      
        1 mkrsvdgrni nqeeiedhdp lqvqqqqeea eqqqqqmeqq taqpqirfls anvlqqlqqq
       61 qdvqqqaqqq qqpqvitlqq lqnfvplqtq qpqhdqqraq tisvqslpqq flqgaqlist
      121 qaqaalqqqq qqsqqqqaqq sqqpqqqqls ysvipqmqtv nidgqealfi pssamsaash
      181 hqsqptmqfa tangqqvqla sqqvqlangq tiitpqpvsl irapnvfpts iiqnitgqtv
      241 qlptgqsvqv rplqfpmqhv qqtvpvqvpv tasngqtvyq tvhfpvqals svfnvpttqm
      301 ipqitqqipq maqiitpngq iqqvqianip qlqslqsqqv tqvaqqvaqq qaqqqvvqqq
      361 aqtqvvqsvq qqqqaaqaqa qqqqqqqqqq qqqvqqvvqq viqqqqqqqq qqqvqqaqqq
      421 sstpsstiat wsttvstpsn vqvlgigalg rsvagngsvi ttkdgqkidv qtlsaltrpp
      481 eavegdikvt sidarqlasg qvihiptaqs tqsavqpiti agaqaqqltl ipasalanlt
      541 aqqgnmmrgv gsggimqlqp agginatngf lqsipvqnip glgnvqvipa salqpatvqt
      601 lpatagapiv aaptvqldsn dptkwqilqt lqsnntlstp apishqhqin ttpstnietd
      661 snkqhrrrva ctcpncgdgd rnrdmtrkrq hvchiagcnk vygktshlra hlrwhtgerp
      721 fvcswifcgk kftrsdelqr hrrthtgekr fqcpectkkf mrsdhltkhi kthtkirste
      781 aatstqegss dsqssteeki iialhkdteq tdmvisepid dikpqtgnhv tgssatenlg
      841 vnvg
//
LOCUS       XP_034190531             844 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608898 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190531
VERSION     XP_034190531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334640.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="uncharacterized protein LOC117608898 isoform X1"
                     /calculated_mol_wt=92587
     Region          151..>327
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          <421..692
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          693..715
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(700,702,704,706..707,710..711,714,730,732,736..737,
                     740..741,744,758,760,762,764..765,768..769,772)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          707..734
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          723..745
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          737..760
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          751..773
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          753..773
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..844
                     /gene="LOC117608898"
                     /coded_by="XM_034334640.2:351..2885"
                     /db_xref="GeneID:117608898"
ORIGIN      
        1 mkrsvdgrni nqeeiedhdp lqvqqqqeea eqqqqqmeqq taqpqirfls anvlqqlqqq
       61 qdvqqqaqqq qqpqvitlqq lqnfvplqtq qpqhdqqraq tisvqslpqq flqgaqlist
      121 qaqaalqqqq qqsqqqqaqq sqqpqqqqls ysvipqmqtv nidgqealfi pssamsaash
      181 hqsqptmqfa tangqqvqla sqqvqlangq tiitpqpvsl irapnvfpts iiqnitgqtv
      241 qlptgqsvqv rplqfpmqhv qqtvpvqvpv tasngqtvyq tvhfpvqals svfnvpttqm
      301 ipqitqqipq maqiitpngq iqqvqianip qlqslqsqqv tqvaqqvaqq qaqqqvvqqq
      361 aqtqvvqsvq qqqqaaqaqa qqqqqqqqqq qqqvqqvvqq viqqqqqqqq qqqvqqaqqq
      421 sstpsstiat wsttvstpsn vqvlgigalg rsvagngsvi ttkdgqkidv qtlsaltrpp
      481 eavegdikvt sidarqlasg qvihiptaqs tqsavqpiti agaqaqqltl ipasalanlt
      541 aqqgnmmrgv gsggimqlqp agginatngf lqsipvqnip glgnvqvipa salqpatvqt
      601 lpatagapiv aaptvqldsn dptkwqilqt lqsnntlstp apishqhqin ttpstnietd
      661 snkqhrrrva ctcpncgdgd rnrdmtrkrq hvchiagcnk vygktshlra hlrwhtgerp
      721 fvcswifcgk kftrsdelqr hrrthtgekr fqcpectkkf mrsdhltkhi kthtkirste
      781 aatstqegss dsqssteeki iialhkdteq tdmvisepid dikpqtgnhv tgssatenlg
      841 vnvg
//
LOCUS       XP_034190532             844 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608898 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190532
VERSION     XP_034190532.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334641.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="uncharacterized protein LOC117608898 isoform X1"
                     /calculated_mol_wt=92587
     Region          151..>327
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          <421..692
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          693..715
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(700,702,704,706..707,710..711,714,730,732,736..737,
                     740..741,744,758,760,762,764..765,768..769,772)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          707..734
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          723..745
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          737..760
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          751..773
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          753..773
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..844
                     /gene="LOC117608898"
                     /coded_by="XM_034334641.2:830..3364"
                     /db_xref="GeneID:117608898"
ORIGIN      
        1 mkrsvdgrni nqeeiedhdp lqvqqqqeea eqqqqqmeqq taqpqirfls anvlqqlqqq
       61 qdvqqqaqqq qqpqvitlqq lqnfvplqtq qpqhdqqraq tisvqslpqq flqgaqlist
      121 qaqaalqqqq qqsqqqqaqq sqqpqqqqls ysvipqmqtv nidgqealfi pssamsaash
      181 hqsqptmqfa tangqqvqla sqqvqlangq tiitpqpvsl irapnvfpts iiqnitgqtv
      241 qlptgqsvqv rplqfpmqhv qqtvpvqvpv tasngqtvyq tvhfpvqals svfnvpttqm
      301 ipqitqqipq maqiitpngq iqqvqianip qlqslqsqqv tqvaqqvaqq qaqqqvvqqq
      361 aqtqvvqsvq qqqqaaqaqa qqqqqqqqqq qqqvqqvvqq viqqqqqqqq qqqvqqaqqq
      421 sstpsstiat wsttvstpsn vqvlgigalg rsvagngsvi ttkdgqkidv qtlsaltrpp
      481 eavegdikvt sidarqlasg qvihiptaqs tqsavqpiti agaqaqqltl ipasalanlt
      541 aqqgnmmrgv gsggimqlqp agginatngf lqsipvqnip glgnvqvipa salqpatvqt
      601 lpatagapiv aaptvqldsn dptkwqilqt lqsnntlstp apishqhqin ttpstnietd
      661 snkqhrrrva ctcpncgdgd rnrdmtrkrq hvchiagcnk vygktshlra hlrwhtgerp
      721 fvcswifcgk kftrsdelqr hrrthtgekr fqcpectkkf mrsdhltkhi kthtkirste
      781 aatstqegss dsqssteeki iialhkdteq tdmvisepid dikpqtgnhv tgssatenlg
      841 vnvg
//
LOCUS       XP_034190533             838 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608898 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190533
VERSION     XP_034190533.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334642.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="uncharacterized protein LOC117608898 isoform X2"
                     /calculated_mol_wt=91915
     Region          151..>333
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          <415..686
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          687..709
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(694,696,698,700..701,704..705,708,724,726,730..731,
                     734..735,738,752,754,756,758..759,762..763,766)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          701..728
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          717..739
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          731..754
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          745..767
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          747..767
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..838
                     /gene="LOC117608898"
                     /coded_by="XM_034334642.2:996..3512"
                     /db_xref="GeneID:117608898"
ORIGIN      
        1 mkrsvdgrni nqeeiedhdp lqvqqqqeea eqqqqqmeqq taqpqirfls anvlqqlqqq
       61 qdvqqqaqqq qqpqvitlqq lqnfvplqtq qpqhdqqraq tisvqslpqq flqgaqlist
      121 qaqaalqqqq qqsqqqqaqq sqqpqqqqls ysvipqmqtv nidgqealfi pssamsaash
      181 hqsqptmqfa tangqqvqla sqqvqlangq tiitpqpvsl irapnvfpts iiqnitgqtv
      241 qlptgqsvqv rplqfpmqhv qqtvpvqvpv tasngqtvyq tvhfpvqals svfnvpttqm
      301 ipqitqqipq maqiitpngq iqqvqianip qlqslqvaqq vaqqqaqqqv vqqqaqtqvv
      361 qsvqqqqqaa qaqaqqqqqq qqqqqqqvqq vvqqviqqqq qqqqqqqvqq aqqqsstpss
      421 tiatwsttvs tpsnvqvlgi galgrsvagn gsvittkdgq kidvqtlsal trppeavegd
      481 ikvtsidarq lasgqvihip taqstqsavq pitiagaqaq qltlipasal anltaqqgnm
      541 mrgvgsggim qlqpaggina tngflqsipv qnipglgnvq vipasalqpa tvqtlpatag
      601 apivaaptvq ldsndptkwq ilqtlqsnnt lstpapishq hqinttpstn ietdsnkqhr
      661 rrvactcpnc gdgdrnrdmt rkrqhvchia gcnkvygkts hlrahlrwht gerpfvcswi
      721 fcgkkftrsd elqrhrrtht gekrfqcpec tkkfmrsdhl tkhikthtki rsteaatstq
      781 egssdsqsst eekiiialhk dteqtdmvis epiddikpqt gnhvtgssat enlgvnvg
//
LOCUS       XP_034190536             720 aa            linear   INV 03-SEP-2025
DEFINITION  glycerophosphate oxidase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190536
VERSION     XP_034190536.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334645.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..720
                     /product="glycerophosphate oxidase 1 isoform X1"
                     /calculated_mol_wt=80177
     Region          1..589
                     /region_name="DAO"
                     /note="FAD dependent oxidoreductase; cl40741"
                     /db_xref="CDD:477422"
     Region          619..679
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(625,627,629,636,662,664,666,673)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     CDS             1..720
                     /gene="Gpo1"
                     /coded_by="XM_034334645.2:212..2374"
                     /db_xref="GeneID:117608900"
ORIGIN      
        1 maslkllagg asaigasalt sylllcdntv hadkprvkpa krplptredq vktlkthgey
       61 dvliigggat gagcaldact rglktalver ddfasgtssr stklihggvr ylqkailqld
      121 veqykmvkea lherasmlhs aphlayplpi mlpvytwwqi pyywfgikmy dvvagsktvk
      181 ssyflsksna lelfpmlkgd kltgaivyyd gqqddarmnl avaltasrhg atvvnhvkvv
      241 nllkgrdkdg nriltgahvk ddltgeewdv kakaiinatg pftdhirkmd dpsipeicsp
      301 ssgahivlpg yyspdqmgll dpatsdgrvi fflpwqkqti agttdlpcev thnprptede
      361 imfilrevkn ylnpdvevrr gdvlsawsgi rplvsdpnkp ntqslarnhi vhvspaklit
      421 iaggkwttyr amaeetidaa iracdlkper pcqtndflle gahgwsptmy irlvqdfgle
      481 cevaqhlars ygdrafavak masltgkrwp iigkkihpef pyidaeiryg vreyartaid
      541 miarrlrlaf lnvqaaqeal psiidimaee lhwtpeekqn qhkeasefla nemghtvnrv
      601 srdkipinlt keeiqlyikr fgiidkdnkg yvsindirrg lklfgdkevp geelheilre
      661 idtnmngqve ldeylqmmsa iksghvaysr farmaemeea ehdkdvlrkk vtversgggl
//
LOCUS       XP_034190537             719 aa            linear   INV 03-SEP-2025
DEFINITION  glycerophosphate oxidase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190537
VERSION     XP_034190537.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334646.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..719
                     /product="glycerophosphate oxidase 1 isoform X2"
                     /calculated_mol_wt=80097
     Region          1..589
                     /region_name="DAO"
                     /note="FAD dependent oxidoreductase; cl40741"
                     /db_xref="CDD:477422"
     Region          619..678
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(625,627,629,636,661,663,665,672)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     CDS             1..719
                     /gene="Gpo1"
                     /coded_by="XM_034334646.2:212..2371"
                     /db_xref="GeneID:117608900"
ORIGIN      
        1 maslkllagg asaigasalt sylllcdntv hadkprvkpa krplptredq vktlkthgey
       61 dvliigggat gagcaldact rglktalver ddfasgtssr stklihggvr ylqkailqld
      121 veqykmvkea lherasmlhs aphlayplpi mlpvytwwqi pyywfgikmy dvvagsktvk
      181 ssyflsksna lelfpmlkgd kltgaivyyd gqqddarmnl avaltasrhg atvvnhvkvv
      241 nllkgrdkdg nriltgahvk ddltgeewdv kakaiinatg pftdhirkmd dpsipeicsp
      301 ssgahivlpg yyspdqmgll dpatsdgrvi fflpwqkqti agttdlpcev thnprptede
      361 imfilrevkn ylnpdvevrr gdvlsawsgi rplvsdpnkp ntqslarnhi vhvspaklit
      421 iaggkwttyr amaeetidaa iracdlkper pcqtndflle gahgwsptmy irlvqdfgle
      481 cevaqhlars ygdrafavak masltgkrwp iigkkihpef pyidaeiryg vreyartaid
      541 miarrlrlaf lnvqaaqeal psiidimaee lhwtpeekqn qhkeasefla nemghtvnrv
      601 srdkipinlt keeiqlyikr fgiidkdnkg yvsindirrg lkvlglklke demhtllnei
      661 dlsyngqmel qdylqmmsai ksghvaysrf armaemeeae hdkdvlrkkv tversgggl
//
LOCUS       XP_034190538             753 aa            linear   INV 03-SEP-2025
DEFINITION  glycerophosphate oxidase 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190538
VERSION     XP_034190538.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334647.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..753
                     /product="glycerophosphate oxidase 1 isoform X3"
                     /calculated_mol_wt=84054
     Region          1..589
                     /region_name="DAO"
                     /note="FAD dependent oxidoreductase; cl40741"
                     /db_xref="CDD:477422"
     Region          605..711
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..753
                     /gene="Gpo1"
                     /coded_by="XM_034334647.2:213..2474"
                     /db_xref="GeneID:117608900"
ORIGIN      
        1 maslkllagg asaigasalt sylllcdntv hadkprvkpa krplptredq vktlkthgey
       61 dvliigggat gagcaldact rglktalver ddfasgtssr stklihggvr ylqkailqld
      121 veqykmvkea lherasmlhs aphlayplpi mlpvytwwqi pyywfgikmy dvvagsktvk
      181 ssyflsksna lelfpmlkgd kltgaivyyd gqqddarmnl avaltasrhg atvvnhvkvv
      241 nllkgrdkdg nriltgahvk ddltgeewdv kakaiinatg pftdhirkmd dpsipeicsp
      301 ssgahivlpg yyspdqmgll dpatsdgrvi fflpwqkqti agttdlpcev thnprptede
      361 imfilrevkn ylnpdvevrr gdvlsawsgi rplvsdpnkp ntqslarnhi vhvspaklit
      421 iaggkwttyr amaeetidaa iracdlkper pcqtndflle gahgwsptmy irlvqdfgle
      481 cevaqhlars ygdrafavak masltgkrwp iigkkihpef pyidaeiryg vreyartaid
      541 miarrlrlaf lnvqaaqeal psiidimaee lhwtpeekqn qhkeasefla nemghtvnrv
      601 srdkipinlt keeiqlyikr fgiidkdnkg yvsindirrg lkvlglklke demhtllnei
      661 dlsyngqmel qdylqlfgdk evpgeelhei lreidtnmng qveldeylqm msaiksghva
      721 ysrfarmaem eeaehdkdvl rkkvtversg ggl
//
LOCUS       XP_034190539             717 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family F member 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190539
VERSION     XP_034190539.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334648.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190539.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..717
                     /product="ATP-binding cassette sub-family F member 3
                     isoform X1"
                     /calculated_mol_wt=80453
     Region          1..712
                     /region_name="PLN03073"
                     /note="ABC transporter F family; Provisional"
                     /db_xref="CDD:215558"
     CDS             1..717
                     /gene="LOC117608901"
                     /coded_by="XM_034334648.2:635..2788"
                     /db_xref="GeneID:117608901"
ORIGIN      
        1 mavcgeyirs qfptidddly qyvegildss kddfedgdev yeaigevlhe veekpenevr
       61 qicvklleml kgnsndenee rkkngvnkvl napihlgsma atleaqveqi ksiwvttrdd
      121 amkvdakkle kaeaklqqkq ekrnnnestg rvntvscgie sasasqmtsk kdsrmenkgs
      181 vnktqdirie nfdvaygdri llqgadltla fgrrygligr nglgkttllr misskqlrip
      241 shirvlhveq evagdetsal esvlecdqer smllskeael qaaieknvka rdtlgeelar
      301 vyeamqlaev dkaparasti ltglgftver qswptkafsg gwrmrlalar alfsrpdlll
      361 ldeptnmldi kailwlekyl qswpttllvv shdrnfldtv ptdilylkgq kieayrgnye
      421 qfsktkgere rnqqreyeaq qakrahvqef idrfrynanr assvqskikm lnkleelkpm
      481 ekegevtlrf pdveplsppi lqlnevsfsy tggsdesyil rgvnltaglq sricivgeng
      541 agkttllkii tgalsptrgt vhvhrnlkfg yfsqhhvdql dmqscpvell qkhfpgkpve
      601 eyrrmlgsfg vsgklalqsi sslsggqksr vafalmsaat pnflvldept nhldiesiea
      661 lgkalntcqa gvilvshder lirmvctelw vctqgsvrci eggfdeyrri iekelev
//
LOCUS       XP_034190541             659 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family F member 3 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190541
VERSION     XP_034190541.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334650.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190541.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..659
                     /product="ATP-binding cassette sub-family F member 3
                     isoform X2"
                     /calculated_mol_wt=73828
     Region          2..654
                     /region_name="PLN03073"
                     /note="ABC transporter F family; Provisional"
                     /db_xref="CDD:215558"
     CDS             1..659
                     /gene="LOC117608901"
                     /coded_by="XM_034334650.2:116..2095"
                     /db_xref="GeneID:117608901"
ORIGIN      
        1 mrqicvklle mlkgnsnden eerkkngvnk vlnapihlgs maatleaqve qiksiwvttr
       61 ddamkvdakk lekaeaklqq kqekrnnnes tgrvntvscg iesasasqmt skkdsrmenk
      121 gsvnktqdir ienfdvaygd rillqgadlt lafgrrygli grnglgkttl lrmisskqlr
      181 ipshirvlhv eqevagdets alesvlecdq ersmllskea elqaaieknv kardtlgeel
      241 arvyeamqla evdkaparas tiltglgftv erqswptkaf sggwrmrlal aralfsrpdl
      301 llldeptnml dikailwlek ylqswpttll vvshdrnfld tvptdilylk gqkieayrgn
      361 yeqfsktkge rernqqreye aqqakrahvq efidrfryna nrassvqski kmlnkleelk
      421 pmekegevtl rfpdveplsp pilqlnevsf sytggsdesy ilrgvnltag lqsricivge
      481 ngagkttllk iitgalsptr gtvhvhrnlk fgyfsqhhvd qldmqscpve llqkhfpgkp
      541 veeyrrmlgs fgvsgklalq sisslsggqk srvafalmsa atpnflvlde ptnhldiesi
      601 ealgkalntc qagvilvshd erlirmvcte lwvctqgsvr cieggfdeyr riiekelev
//
LOCUS       XP_034190225             574 aa            linear   INV 03-SEP-2025
DEFINITION  vasoactive intestinal polypeptide receptor 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190225
VERSION     XP_034190225.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334334.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..574
                     /product="vasoactive intestinal polypeptide receptor 2"
                     /calculated_mol_wt=65684
     Region          40..105
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          129..413
                     /region_name="7tmB1_NPR_B7_insect-like"
                     /note="insect neuropeptide receptor subgroup B7 and
                     related proteins, member of the class B family of
                     seven-transmembrane G protein-coupled receptors; cd15273"
                     /db_xref="CDD:320401"
     Region          131..156
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320401"
     Site            order(136,140,182,185..186,189,219,226,298..299,301,303,
                     357,360,378,382)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:320401"
     Region          165..187
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320401"
     Region          219..246
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320401"
     Region          259..279
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320401"
     Region          295..324
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320401"
     Region          338..365
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320401"
     Region          375..400
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320401"
     CDS             1..574
                     /gene="LOC117608760"
                     /coded_by="XM_034334334.2:5886..7610"
                     /db_xref="GeneID:117608760"
ORIGIN      
        1 mdlykdfdit nltnqdiqkr ilkikadqch kgnismpgwc peiwdeilcw pstapgdlai
       61 lscpsyvagf efqanaskqc mpdgqwfwnn gtnslwsnys qcyrnplvtl vmklpggqen
      121 ndtlikkfvp ivktiskvgy tvsfftlvva ffilavikkl rcprnmlhmh lfasfmlraf
      181 mallkdilfv sgialasdlm iingetywlv aeestwmckv fisfwqyfil anyfwilmeg
      241 lylhnlvffa lftdvnssii gyvclgwglp amfvscwiva rvmldnsycw ttheypylfl
      301 lirvpttlsi linfvlfvni vrvlllklks tvseetqryk rwakstlvlv plfgvhytlf
      361 lgmsysigmn etvevvwlfc dqlfasfqgf fvavlycfln gevrtevsra irsqrlprvr
      421 grwisrrfah sgstyscngs klgkrskqpr wwkdpwlcfl hdrtarrsth smastqdigt
      481 rggslassrg yleiagnaqg ahpgnesqqr anhtsplysk ytdqsllsfc snvseatpct
      541 gsnverfvrd qhrwsdsecc hlayelhnlh hgpp
//
LOCUS       XP_034190542             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190542
VERSION     XP_034190542.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334651.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334651.2:731..2212"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190543             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190543
VERSION     XP_034190543.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334652.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334652.2:727..2208"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190544             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190544
VERSION     XP_034190544.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334653.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334653.2:157..1638"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190548             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190548
VERSION     XP_034190548.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334657.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334657.2:92..1573"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190547             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190547
VERSION     XP_034190547.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334656.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334656.2:124..1605"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190549             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190549
VERSION     XP_034190549.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334658.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334658.2:490..1971"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190545             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190545
VERSION     XP_034190545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334654.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334654.2:160..1641"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190550             493 aa            linear   INV 03-SEP-2025
DEFINITION  protein coronin [Osmia lignaria lignaria].
ACCESSION   XP_034190550
VERSION     XP_034190550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334659.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="protein coronin"
                     /calculated_mol_wt=54615
     Region          9..488
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          37..78
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          85..129
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          134..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          178..213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          220..260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          267..308
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..493
                     /gene="coro"
                     /coded_by="XM_034334659.2:487..1968"
                     /db_xref="GeneID:117608902"
ORIGIN      
        1 msfrvvrssk frhvygtalk reqcydnirv sksswdstfc avnpkflaii vesagggafi
       61 vlphnkvgri padyplvggh kgpvldiawc phndnviasg sedcvvkvwq ipdggisrtl
      121 tesvvdlqlh qrrvglvlwh psalnvllta gsdnlvliwn vgtgeamvri dchpdmiysa
      181 cwnwdgsrlv ttckdkkiri ldprtgemle eaiahegska traiflrggl ifttgfskms
      241 erqyslrapd mlgepivmve ldtsngvmfp lydsdtnlvy lcgkgdsvir yfeitpeppf
      301 vhyintfqtp dpqrgigmmp krgcdvnsce itrfyrlnns gfcqvismtv prkselfqed
      361 lypdtpgdta aisaeewekg tdadpvlisl rdgyqpsatk nelkvhkksn ilskgakvas
      421 nssqnatqas agiseellke fteeirklka vivkhegrir vlesavalqm kederkekss
      481 tptdrevlas dev
//
LOCUS       XP_034190551             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608903 [Osmia lignaria lignaria].
ACCESSION   XP_034190551
VERSION     XP_034190551.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC117608903"
                     /calculated_mol_wt=55138
     Region          301..436
                     /region_name="TLD"
                     /note="cl02144"
                     /db_xref="CDD:445683"
     CDS             1..490
                     /gene="LOC117608903"
                     /coded_by="XM_034334660.2:148..1620"
                     /db_xref="GeneID:117608903"
ORIGIN      
        1 mgnqvtragh astkktdsta hagdtanans lsksssgtel esnshmqffp iesltktlsh
       61 ltyeedrvhg itksvfqryl fpnypdlgdk lfdflhytan vntshlstna fkhqaekfls
      121 vmndqtvlen yvrmysclkg dgsvnpdglk allnisykia mdnsgtpsci fadqiinavv
      181 lscfhgkyal svsyvsnwiw qnctrivqgp hryiihvltt ayrsgkalls keqpqpllei
      241 ptpileqpes iefprvllpl syvwllactl pqcylqldds pkdvthalia krmgtvcprh
      301 wtllynsaeh gtganrflhh vlgyrgptll firaispdkd tvcptycics avewreshly
      361 wgdensmgie lfpsyrviek gpkvlylntn irgyphglrf gsnprspyis idesfhsvsi
      421 agapypiasl evwgcgdtkl rerqleikkw qvkeaekqri vklsasdwid hpdryllela
      481 grasynesnk
//
LOCUS       XP_034190552             395 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608904 [Osmia lignaria lignaria].
ACCESSION   XP_034190552
VERSION     XP_034190552.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334661.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..395
                     /product="uncharacterized protein LOC117608904"
                     /calculated_mol_wt=40761
     Region          21..50
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(23..24,26..28,30..32,37..40)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          <159..>245
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..395
                     /gene="LOC117608904"
                     /coded_by="XM_034334661.2:432..1619"
                     /db_xref="GeneID:117608904"
ORIGIN      
        1 mpappppppp vfnasssgag eqdrslllqs iragkalkkt vtvdksapvv sgrvkgepgn
       61 sssnnrennn ssaiaggint snggpiglgg lfssgmpklk ptglrassge kdtsnvnntn
      121 fgqtttssvk rgpppvpppa tqkpqmfaqk tsslpgqstt dsgssnseap kgfgkptvap
      181 kppptssatl hkpspppkkl nltsggsvsr aqsmrlprsp pvlaptppsl hqsqdylnes
      241 qprptnrilr ppvakppspp tsrannsttt atrvapppps rvtvsapcip ppppplphrp
      301 apthqrlapp ppppptpptr sssmrngqsm stldievrfa dmfhsiasfp ppeafkgftk
      361 vyssrnaakq qapappmqms slsntmvpln tssgg
//
LOCUS       XP_034190226             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608761 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190226
VERSION     XP_034190226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334335.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117608761 isoform X1"
                     /calculated_mol_wt=35403
     Region          102..303
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..320
                     /gene="LOC117608761"
                     /coded_by="XM_034334335.2:134..1096"
                     /db_xref="GeneID:117608761"
ORIGIN      
        1 mrfhldrgmi llialalqav ssnsdepvps iidgignvan satnlltgim qrqievihrv
       61 tesasslisn avekprklli natrvttgyi dsaaskglef klrrivekfr armpygipel
      121 dippleplkl devhinidnp eigmmslvie dlvvhnlstf vtneaklsll gptidlnltv
      181 pkiyaegyyn isgvlggmvh lhgagsfqan iynfmlyvht vlgysrgvyl kmfdvdfwlt
      241 smhvnlenfm gdkeladlms kvfqdltpkv vdiikpdvlp girddiggri netihnltmk
      301 dvitvlvghn dirdlvhlvp
//
LOCUS       XP_034190227             298 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608761 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190227
VERSION     XP_034190227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334336.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="uncharacterized protein LOC117608761 isoform X2"
                     /calculated_mol_wt=32952
     Region          80..281
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..298
                     /gene="LOC117608761"
                     /coded_by="XM_034334336.2:132..1028"
                     /db_xref="GeneID:117608761"
ORIGIN      
        1 mrfhldrgmi llialalqav ssnsdepvps iidgignvan satnllsnav ekprkllina
       61 trvttgyids aaskglefkl rrivekfrar mpygipeldi ppleplklde vhinidnpei
      121 gmmslviedl vvhnlstfvt neaklsllgp tidlnltvpk iyaegyynis gvlggmvhlh
      181 gagsfqaniy nfmlyvhtvl gysrgvylkm fdvdfwltsm hvnlenfmgd keladlmskv
      241 fqdltpkvvd iikpdvlpgi rddiggrine tihnltmkdv itvlvghndi rdlvhlvp
//
LOCUS       XP_034190229             257 aa            linear   INV 03-SEP-2025
DEFINITION  protein obstructor-E [Osmia lignaria lignaria].
ACCESSION   XP_034190229
VERSION     XP_034190229.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190229.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..257
                     /product="protein obstructor-E"
                     /calculated_mol_wt=28561
     Region          49..101
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          <132..170
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          195..240
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..257
                     /gene="LOC117608762"
                     /coded_by="XM_034334338.2:314..1087"
                     /db_xref="GeneID:117608762"
ORIGIN      
        1 mpsktivhlv iacgllsrie sasqyhvlsq fgqsnqvdhs issrqhsscp ekngrfpvps
       61 qcdayiecid gvaeeklcpe gllfnpdarf nypcgypidi dcegrqsrqs pqptddcphq
      121 ygyfkvgdht hcgqfvncaa gigykfdcpe glafnpesyr cdwpdqvpdc daeaflgfqc
      181 pedtsnsyyg neikfyrsph dcqryyvcvn gkprlqncge gnafnelina cdgaenvtgc
      241 eppestildr tkkplrl
//
LOCUS       XP_034190554             131 aa            linear   INV 03-SEP-2025
DEFINITION  prefoldin subunit 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190554
VERSION     XP_034190554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334663.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..131
                     /product="prefoldin subunit 1 isoform X1"
                     /calculated_mol_wt=15446
     Region          20..118
                     /region_name="Prefoldin_1"
                     /note="prefoldin subunit 1; cd23164"
                     /db_xref="CDD:467480"
     Site            order(20..21,24,31,34,95,98..99,101..102,104..106,
                     108..109,112..113,115..116)
                     /site_type="other"
                     /note="prefoldin beta subunit /TRiC interface [polypeptide
                     binding]"
                     /db_xref="CDD:467480"
     Region          20..59
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467480"
     Site            order(55..56,59,61..62,64..66,68,73)
                     /site_type="other"
                     /note="prefoldin oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467480"
     Region          79..118
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467480"
     CDS             1..131
                     /gene="Pfdn1"
                     /coded_by="XM_034334663.2:216..611"
                     /db_xref="GeneID:117608906"
ORIGIN      
        1 myehimarep deelrkafsq lhekvidttq klkladlqie klkrtkqrae ltmkeissyp
       61 kntktyesvg rmfllddidn ikaeldkrmk nadekvktle nnktylqqnl kesennirem
      121 iqqrqnkdts g
//
LOCUS       XP_034190555             126 aa            linear   INV 03-SEP-2025
DEFINITION  prefoldin subunit 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190555
VERSION     XP_034190555.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334664.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..126
                     /product="prefoldin subunit 1 isoform X2"
                     /calculated_mol_wt=14772
     Region          15..113
                     /region_name="Prefoldin_1"
                     /note="prefoldin subunit 1; cd23164"
                     /db_xref="CDD:467480"
     Site            order(15..16,19,26,29,90,93..94,96..97,99..101,103..104,
                     107..108,110..111)
                     /site_type="other"
                     /note="prefoldin beta subunit /TRiC interface [polypeptide
                     binding]"
                     /db_xref="CDD:467480"
     Region          15..54
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467480"
     Site            order(50..51,54,56..57,59..61,63,68)
                     /site_type="other"
                     /note="prefoldin oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467480"
     Region          74..113
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467480"
     CDS             1..126
                     /gene="Pfdn1"
                     /coded_by="XM_034334664.2:222..602"
                     /db_xref="GeneID:117608906"
ORIGIN      
        1 marepdeelr kafsqlhekv idttqklkla dlqieklkrt kqraeltmke issypkntkt
       61 yesvgrmfll ddidnikael dkrmknadek vktlennkty lqqnlkesen niremiqqrq
      121 nkdtsg
//
LOCUS       XP_034190553             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608905 [Osmia lignaria lignaria].
ACCESSION   XP_034190553
VERSION     XP_034190553.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334662.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190553.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117608905"
                     /calculated_mol_wt=25885
     CDS             1..228
                     /gene="LOC117608905"
                     /coded_by="XM_034334662.2:28..714"
                     /db_xref="GeneID:117608905"
ORIGIN      
        1 matdieesdt ddherpappk rqctrlvkvn etmwdmensl neseeiqdae nveyddaerh
       61 areklkrwqa cqvakgyvdn tinkflenyi trtisleesr cqlfrgndme dtavmmairn
      121 hglvqsagiv sqsdafysdk apgywtnney pdvhcscpsn yiqvgnsvat tstdslrled
      181 skdvdeysgc dwdldkiden nqqenflera vaeaikkkgl stlsvdyg
//
LOCUS       XP_076545658             629 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305523 [Osmia lignaria lignaria].
ACCESSION   XP_076545658
VERSION     XP_076545658.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689543.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="uncharacterized protein LOC143305523"
                     /calculated_mol_wt=72313
     Region          <1..334
                     /region_name="RecD"
                     /note="ATPase/5#-3# helicase helicase subunit RecD of the
                     DNA repair enzyme RecBCD (exonuclease V) [Replication,
                     recombination and repair]; COG0507"
                     /db_xref="CDD:440273"
     Region          494..614
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     CDS             1..629
                     /gene="LOC143305523"
                     /coded_by="XM_076689543.1:30..1919"
                     /db_xref="GeneID:143305523"
ORIGIN      
        1 migsdifhtv nsrlqqitgn ddkpfggqni ifcgdlrqlp pikgtpiykr ygkringeml
       61 wqrlyyypli qimrqsniql smiltkigng eslnleetma iekrfideda arqlkpsair
      121 lflrnddvsr ynasvlkknr dtvtftaidr yegyrsneql lsmqtkvqkm klnetsglpc
      181 elslligapy mitsnidvvd glvngaignl myieydendp drsikhlwfd fgkgkvgqlq
      241 kakwetyirs hkslqdnwva vrkrtiditf sskaltcrrq qfplleayal tihksqgatf
      301 deivyeysks hvqelvyval srvtslqely ltnknkhytf yhtrgnvnna lssefrrlen
      361 hrlptvtdec ekflertran dlthttlnvq slkshkydva qdfllkttkm malsetwmdn
      421 neiidlegyh cinqfkrenv rpggvalyem ndselmarkh tlvkvdakid srvlaveqcg
      481 dicaaeacin rrqvlivvfy ispgtslqdt kdffevnlla yniraaqlfe slqlcgydkl
      541 piiisgdlnl nlqtpegrdf idfmecslnl klvndsskct tlggttvdav ftrymdnvhc
      601 rnyvnyfsyh qpilcttnes fnshdknfk
//
LOCUS       XP_034185457             629 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606730 [Osmia lignaria lignaria].
ACCESSION   XP_034185457
VERSION     XP_034185457.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329566.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185457.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="uncharacterized protein LOC117606730"
                     /calculated_mol_wt=70896
     Region          42..99
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          <377..593
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..629
                     /gene="LOC117606730"
                     /coded_by="XM_034329566.2:86..1975"
                     /db_xref="GeneID:117606730"
ORIGIN      
        1 meqtrrllfl awvavfwtsa lglqrpppry sqqempetsf scrnkivgsy yadaetdcqv
       61 fhvcvsvsgt vqdykflcpn dtafdqesqt cadwydvdce aatlyyasdn fdlyrigsgl
      121 eslhydsirt daepqdhlqr setsdpvrss annlnrisps ynsnnnnnrd lrgsntgnfy
      181 nrngkeddye ndksykdevv qetkkktgvr kvsrkqqlne nnyvssstpa papisnqnty
      241 tgfysgnnyn qrangfvqst ssrpqenynr nqngqvynvp sstykpstny qnnynfqsps
      301 ttskttiyna ynqnynnqqn ngnfvqstta kpanynknyq sysngqfslp ttlqpstnyq
      361 andytnyqrn ynniqrtngn nvpyptttas pvydntyssn nngqyraats trqdvaqttk
      421 yfpsfasssy apttyspatk kyidnsqaqt vtrntengqy yekssqsiks ssqyydstkt
      481 pkkatqynyd ngkyytesst snyefarssv gigfspasin hlaetprstt pvprrvstvt
      541 tynpnsfnsn tqkasqpqtt prastyvsgs arpfssttrl assttprpks gkkndydyay
      601 ydntagleyd gldlehvtgn kestkiarn
//
LOCUS       XP_034195083            2236 aa            linear   INV 03-SEP-2025
DEFINITION  cilia and flagella-associated protein 47 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195083
VERSION     XP_034195083.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339192.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195083.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2236
                     /product="cilia and flagella-associated protein 47 isoform
                     X1"
                     /calculated_mol_wt=255017
     Region          1718..1842
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Site            order(1721,1797,1799..1800,1806..1807,1809,1821..1825,
                     1830,1832..1833,1835..1836,1839..1840)
                     /site_type="other"
                     /note="actin binding site [polypeptide binding]"
                     /db_xref="CDD:409031"
     CDS             1..2236
                     /gene="LOC117611256"
                     /coded_by="XM_034339192.2:246..6956"
                     /db_xref="GeneID:117611256"
ORIGIN      
        1 msntlggten qlstsiiliq egvevssvrd sndavpsavh dnvhitpsyi efkeasegit
       61 crqlitiknt glkpafvklr qpysiafqvk ilkngvmlnp glsvsttviy sfkrpsflra
      121 mipieingks ldyrvvckll eegisvepkt idfgildigy ssgikvltmr ndggkstrfs
      181 idlgkndlei avkplrgvvt pnrpvqltve ligmqegvfy sefwiksspn iriplkvnvi
      241 apklvvyhpn ttgdftlidf siavantrky dsfvlrnvss qvasyvvlge ienevkcird
      301 idrekypvyn afeirpiqgr mdpmegiife vgftpttkll rwrqkvkekt drpendfmaf
      361 lrivrvhvte tkniprsdam realvnesgt psshdvnagl tvmnistsss ssstimergv
      421 hdtirlclhg eveeaclkfe pdivyfgeis vdeisqrvvr ilnplkfapi lyrfvpntav
      481 rcypkrmeik aegsievllk mrgkenvfsp fkiyfdaivd syestktrnk iklkvgtypv
      541 qcvvsiillt knlqephdhs rgirkpllpq fldtmksqvp eepdreildr llrlpkwkye
      601 dmfktvkcns gldkkevlps sintevlipl splqiynvrv ypsiftfgmv vlkshsyrql
      661 ivenmndfpv mirltslsrc iffpdgnvii lpagalvtrl vefrahhigk fngyidyiin
      721 dnhsfelgvi adivrkqlsl etreatlgte wlkeevyrpl ditlqirnkl naktyfrwev
      781 patcgffiep vsgairgnss lfcyayykph airshntemt lkceggtflt vrlnvpiqlp
      841 kvsfiedtin vgkiplnlpt etiallsnvd yveaayevdv sslmygcsvy pltgiimprg
      901 mvgleitltf ctcfdfvivi rvtiqgclhl qlkimgnvtf pqlklypqsi slrrisaaay
      961 qhflitatnv gttmlklnfl lqdypefris lspdrtdpgl gmgnvfimag tsrslhlhfe
     1021 pidlasyafy lpivindilg paletdprtl kpleylkpfk kyyvnltkdd qqdiplklvt
     1081 isidctvagh vvhfsklefr fnistnevee fcienrreak eksirietnn fsvpdcpfsi
     1141 qwsdgkeati ttdsiectlk qgekcvflia fhpkssgnfn leapifvnne ldgemfnkls
     1201 lngeypvrsi gasiseiyft pvpldvaier glrlivtnfe nestifakvs tpdcctgifk
     1261 qemlsinfin gnsipaseyt qlevkivfks dtsvsfrlii efsddsglav cpvmiyatad
     1321 nclltthvyl rtpksdfewf adqdkrishi sklsdtmtdd eeyendgqas ysarhrqtsg
     1381 prisilsyie qysegsvkkl lesrfklsln ksvfmemneq dayykseedp kkfhlesrrs
     1441 skissfyykk yfeqleypff psnnerddye lhmqqtlvaa eewlytgpfk fnfypevpng
     1501 itvsmskfyp kkqaqlksks qkrrsfewsf lnvlellsdq tlynyiekpk tlpddevera
     1561 hflfhhfesi lnylllsgah vchvsaqfll syddyvivte niksmkkvrr ssitiwtgte
     1621 klsprlfesr skqcwldvil qtykcfvlts isqikvrkes fsvstppsrr stvhrasvit
     1681 ssllnlpkqc eihdrnirdm vdlsgdrksr tncyscqeif lmawleyhye qertkewmtd
     1741 rrlilnpnes kdvaearsir nfdadladsl vlaavtaahc pflieecfns iyfypqnfee
     1801 ilhnavcvvn awrkirlgfv ispleivypn cikmlllviy lyktlptyip ktkikfscal
     1861 cevatkqinf snptadnvgf lillfgnsng ffmtetpkpi irlgahesav vklkfhakki
     1921 kktkahllfc gstigphfgr nqvfvlegqa dtlgianeym insklyevid kilsinvpyk
     1981 dpadyeiwms derpskpsal kltrwadlrs rkiprrlfln qtfitvkegs nhgslsltla
     2041 cimpidrdfw ivfqskmgdf iiqissrckp siidnliitl slqedqctct dtytdfsksc
     2101 pfslmvpipc knkqlwssva ymfqkaldqq erifwsryld tyiglhlirw lmgghvtmef
     2161 shvfdrtvty nvtipkaagi ldvpktftin dvmtstqsvp mnvhiapftg rllettltlt
     2221 svngkelrky tvsflk
//
LOCUS       XP_034195084            2145 aa            linear   INV 03-SEP-2025
DEFINITION  cilia and flagella-associated protein 47 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195084
VERSION     XP_034195084.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195084.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2145
                     /product="cilia and flagella-associated protein 47 isoform
                     X2"
                     /calculated_mol_wt=244762
     Region          1718..1842
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Site            order(1721,1797,1799..1800,1806..1807,1809,1821..1825,
                     1830,1832..1833,1835..1836,1839..1840)
                     /site_type="other"
                     /note="actin binding site [polypeptide binding]"
                     /db_xref="CDD:409031"
     CDS             1..2145
                     /gene="LOC117611256"
                     /coded_by="XM_034339193.2:246..6683"
                     /db_xref="GeneID:117611256"
ORIGIN      
        1 msntlggten qlstsiiliq egvevssvrd sndavpsavh dnvhitpsyi efkeasegit
       61 crqlitiknt glkpafvklr qpysiafqvk ilkngvmlnp glsvsttviy sfkrpsflra
      121 mipieingks ldyrvvckll eegisvepkt idfgildigy ssgikvltmr ndggkstrfs
      181 idlgkndlei avkplrgvvt pnrpvqltve ligmqegvfy sefwiksspn iriplkvnvi
      241 apklvvyhpn ttgdftlidf siavantrky dsfvlrnvss qvasyvvlge ienevkcird
      301 idrekypvyn afeirpiqgr mdpmegiife vgftpttkll rwrqkvkekt drpendfmaf
      361 lrivrvhvte tkniprsdam realvnesgt psshdvnagl tvmnistsss ssstimergv
      421 hdtirlclhg eveeaclkfe pdivyfgeis vdeisqrvvr ilnplkfapi lyrfvpntav
      481 rcypkrmeik aegsievllk mrgkenvfsp fkiyfdaivd syestktrnk iklkvgtypv
      541 qcvvsiillt knlqephdhs rgirkpllpq fldtmksqvp eepdreildr llrlpkwkye
      601 dmfktvkcns gldkkevlps sintevlipl splqiynvrv ypsiftfgmv vlkshsyrql
      661 ivenmndfpv mirltslsrc iffpdgnvii lpagalvtrl vefrahhigk fngyidyiin
      721 dnhsfelgvi adivrkqlsl etreatlgte wlkeevyrpl ditlqirnkl naktyfrwev
      781 patcgffiep vsgairgnss lfcyayykph airshntemt lkceggtflt vrlnvpiqlp
      841 kvsfiedtin vgkiplnlpt etiallsnvd yveaayevdv sslmygcsvy pltgiimprg
      901 mvgleitltf ctcfdfvivi rvtiqgclhl qlkimgnvtf pqlklypqsi slrrisaaay
      961 qhflitatnv gttmlklnfl lqdypefris lspdrtdpgl gmgnvfimag tsrslhlhfe
     1021 pidlasyafy lpivindilg paletdprtl kpleylkpfk kyyvnltkdd qqdiplklvt
     1081 isidctvagh vvhfsklefr fnistnevee fcienrreak eksirietnn fsvpdcpfsi
     1141 qwsdgkeati ttdsiectlk qgekcvflia fhpkssgnfn leapifvnne ldgemfnkls
     1201 lngeypvrsi gasiseiyft pvpldvaier glrlivtnfe nestifakvs tpdcctgifk
     1261 qemlsinfin gnsipaseyt qlevkivfks dtsvsfrlii efsddsglav cpvmiyatad
     1321 nclltthvyl rtpksdfewf adqdkrishi sklsdtmtdd eeyendgqas ysarhrqtsg
     1381 prisilsyie qysegsvkkl lesrfklsln ksvfmemneq dayykseedp kkfhlesrrs
     1441 skissfyykk yfeqleypff psnnerddye lhmqqtlvaa eewlytgpfk fnfypevpng
     1501 itvsmskfyp kkqaqlksks qkrrsfewsf lnvlellsdq tlynyiekpk tlpddevera
     1561 hflfhhfesi lnylllsgah vchvsaqfll syddyvivte niksmkkvrr ssitiwtgte
     1621 klsprlfesr skqcwldvil qtykcfvlts isqikvrkes fsvstppsrr stvhrasvit
     1681 ssllnlpkqc eihdrnirdm vdlsgdrksr tncyscqeif lmawleyhye qertkewmtd
     1741 rrlilnpnes kdvaearsir nfdadladsl vlaavtaahc pflieecfns iyfypqnfee
     1801 ilhnavcvvn awrkirlgfv ispleivypn cikmlllviy lyktlptyip ktkikfscal
     1861 cevatkqinf snptadnvgf lillfgnsng ffmtetpkpi irlgahesav vklkfhakki
     1921 kktkahllfc gstigphfgr nqvfvlegqa dtlgianeym insklyevid kilsinvpyk
     1981 dpadyeiwms derpskpsal kltrwadlrs rkiprrlfln qtfitvkegs nhgslsltla
     2041 cimpidrdfw ivfqskmgdf iiqissrckp siidnliitl slqedqctct dtytdfsksc
     2101 pfslmvpipc knkqlwssva ymfqkaldqq erifwsrylg qllit
//
LOCUS       XP_076545659            1902 aa            linear   INV 03-SEP-2025
DEFINITION  cadherin 87A [Osmia lignaria lignaria].
ACCESSION   XP_076545659
VERSION     XP_076545659.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689544.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1902
                     /product="cadherin 87A"
                     /calculated_mol_wt=209250
     Region          78..167
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(80..81,127,129,163,165..166)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          175..277
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          287..392
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(294..295,346,348,389,391..392)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          401..509
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(408..409,469,471,505,507..508)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          517..622
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(524..525,586,588,619,621..622)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          632..727
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(638..639,693,695,723,725..726)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          736..830
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(742..743,794,796,827,829..830)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          839..950
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(846..847,914,916,948,950)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          967..1057
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(973..974,1021,1023,1054,1056..1057)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1067..1166
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1073..1074,1127,1129,1162,1164..1165)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1175..1273
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1181..1182,1237,1269,1271..1272)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1286..1387
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1291..1292,1349,1351,1383,1385..1386)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1394..1505
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1401..1402,1454,1456,1501,1503..1504)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1522..1616
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     CDS             1..1902
                     /gene="Cad87A"
                     /coded_by="XM_076689544.1:696..6404"
                     /db_xref="GeneID:117611257"
ORIGIN      
        1 mtdrhaeraa ircrwtqcqt mgsrtdtrvh siqmfstrfs kklatllavc llatcvgann
       61 vneppilegg gypaglplse stkvgtevfv lkghdpegsp vkygiqltdh fvvnprtgvi
      121 tlakplnree ndtirfritl edevptgqqp nivgvdayvi vldendnppr flgvpyeava
      181 aedlpvgsti lpgirvmdpd llgdtidltc vpqpqfldac ekfsivnver sqnayvgalv
      241 lqqpldyrer pfyqlllras dglnnettgv eikvadiqns ppefldsltg vvredapigt
      301 lvmtvkardg drgmprkmiy elvnnpfdyf llddetgelr takplnreal kdstgvlklk
      361 vkarelidgi pgndnltvst aeatvtikdv ndepptfnhr eynieipenv mngtplpnld
      421 mtvrdpdigl nsvfslrled itdgaftiep tiatgstsvn irvangsldy enpnqrkfii
      481 lvvaeevhtn pklsstatvt isitdvndns ptfssptyta ivsetatpgv piipitakdr
      541 dsgrfgtagi ayqllgqgaq hfsvdkktgm itvapcptpg tspcldyeeq teyfltykat
      601 dddgqgqtas vslrihllda ndspprflqd kyravidega dkfepelivr ardkdktski
      661 tyaivggnel gffaidqdsg evtvkskgll nltnsthdwi aldvqandgi fvdttivnit
      721 irdvnnnapv fphdlytasi peispigtvv eevtatdadt ginaeliyri qkgafddfti
      781 nettgvvtvf rkldydnknt yhievialdk gtpsltgttt lminvensnd kapyftpetq
      841 raevtedtpi gtvfttlkat dpdsasleal nfamsepita idrngqrvnd sksfkdffav
      901 dratgqvsvv rpldrdaaat isitivvsdt taptlqqgrg tlvvtiidvn hlapeflkpw
      961 trenprylie mqeeqptgav vgaftavdad nniagyaivp pspyftidnv tgivrtsqvi
     1021 dyeetkqlef nvvaydsgvp qlsatakvti tvinvndenp kfekelynas veenspagth
     1081 vitvkatdgd egpfgvvnys ligdhaadfn ighdtgeitv gsatvldrem tskititvla
     1141 sdgapinscr sstvpvvvkl idvndnrpif tqhryrasva enlsvnppap ilqvraadhd
     1201 egingevwyt imggnenesf slnhetgily pgaallgrag sytinieard gagrgphsdr
     1261 cyvdirvtpv nqhkpqfvlp eltnatvevp entgvsnyli ltvkaidrdp gengrisyhl
     1321 kvgnrnvqet eefsidqetg elrskiildr eakskfelvl vaadhgtpta yetlrlltiq
     1381 lvdtndnapl fydeyhfhvs enrpkdyfig kvtaedkdeg rhakvyyyie agnedyafyl
     1441 dksdgsiyan ksfnrevrdk yvllilasnd pniyinaada grsmahstdh ghanvtitil
     1501 dendnppife rndyyagins manindfvtk vtasdldvgd ngtlhyyias anlykygsdk
     1561 psgsivpspf nvtqdgkilt sgymaeynqh rfiveviake iviperyamt rvhvwvfept
     1621 qlirvilsrp peevnrerde fvselsnatq srvvvddiry hvdasghirr ewcdmylhvv
     1681 eprtntiapv pqvlkvidak ydflkdyyag faienvvpay agvqeesfdp alaaliallv
     1741 vllvgavtvt vvccclrhwv itvpndirkk dglikkqiid elnttenplw ieqklklyee
     1801 qeltmqvfse pegglgterr gsgvsvgigd tsqdntyati qrplptnrhr pttpdyttlp
     1861 gnhnlyesam gfqgstfqps ssvipqltld rdgqpqfvsg li
//
LOCUS       XP_034184658             619 aa            linear   INV 03-SEP-2025
DEFINITION  ras GTPase-activating protein-binding protein 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184658
VERSION     XP_034184658.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328767.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..619
                     /product="ras GTPase-activating protein-binding protein 2
                     isoform X1"
                     /calculated_mol_wt=68035
     Region          8..133
                     /region_name="NTF2"
                     /note="Nuclear transport factor 2 (NTF2) domain plays an
                     important role in the trafficking of macromolecules, ions
                     and small molecules between the cytoplasm and nucleus.
                     This bi-directional transport of macromolecules across the
                     nuclear envelope requires many...; cd00780"
                     /db_xref="CDD:238403"
     Site            order(36,42,50,81,83..84,93,115,126..128,130,132)
                     /site_type="active"
                     /note="TAP/p15 interaction [active]"
                     /db_xref="CDD:238403"
     Site            order(40,42..43,78..79,81,83..84,91,95..97,101,103..106,
                     109,115,126..128,130..133)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238403"
     Site            order(43..44,68,73,96,98,100..101,103,106,129,131,133)
                     /site_type="active"
                     /note="RanGDP-NTF2 interaction [active]"
                     /db_xref="CDD:238403"
     Region          <166..337
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          437..529
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..619
                     /gene="rin"
                     /coded_by="XM_034328767.2:276..2135"
                     /db_xref="GeneID:117606399"
ORIGIN      
        1 mvmeaspspq nvgrefvrqy ytllnqapah lhrfynqhss fvhggldsnr estpaigqkq
       61 ihqkiqqlnf rdchakisqv dsqltlengv vvqvsgelsn agqpmrrftq tfvlaiqapk
      121 tyyvhndifr yqdlifpdee etdvggvegv vgesverege egvrseaeee ehqnrgqqlt
      181 apaastepqt qpplipaqqs vlqqqqmyya pppqqshvhp vmqqvlngsv heetpliaqq
      241 qpqqqqqqqq qqqppppppa qqnqyitest sqtefvqete mdvaaepqqp aeseaqpqtn
      301 etqppeadpy assapesepe hqvsntnvts sgpktyanlv ksfpsttgat spqapklsms
      361 pppmtnlrld drsplhptss gpalsassnv stpqqqthrv gnqqpqqqqh qqqraprgga
      421 vqrdgdrrgt rqsqysdahq lflgnlphna seddlrqvfe sygrvvelri hsksndrckg
      481 gaqgnntgkv pnygfitfed qqvvnkvlqn lpiyypaeng qklnveekkv rprmsmdgsg
      541 grlnsndgnm rsmggqqqqq qqrgpvgyfp ggpggvmrgg qhgtrggrgg fsrggdggrg
      601 gggmrqpgnp snpsyqnrr
//
LOCUS       XP_076545660             619 aa            linear   INV 03-SEP-2025
DEFINITION  ras GTPase-activating protein-binding protein 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545660
VERSION     XP_076545660.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689545.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..619
                     /product="ras GTPase-activating protein-binding protein 2
                     isoform X1"
                     /calculated_mol_wt=68035
     Region          8..133
                     /region_name="NTF2"
                     /note="Nuclear transport factor 2 (NTF2) domain plays an
                     important role in the trafficking of macromolecules, ions
                     and small molecules between the cytoplasm and nucleus.
                     This bi-directional transport of macromolecules across the
                     nuclear envelope requires many...; cd00780"
                     /db_xref="CDD:238403"
     Site            order(36,42,50,81,83..84,93,115,126..128,130,132)
                     /site_type="active"
                     /note="TAP/p15 interaction [active]"
                     /db_xref="CDD:238403"
     Site            order(40,42..43,78..79,81,83..84,91,95..97,101,103..106,
                     109,115,126..128,130..133)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238403"
     Site            order(43..44,68,73,96,98,100..101,103,106,129,131,133)
                     /site_type="active"
                     /note="RanGDP-NTF2 interaction [active]"
                     /db_xref="CDD:238403"
     Region          <166..337
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          437..529
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..619
                     /gene="rin"
                     /coded_by="XM_076689545.1:355..2214"
                     /db_xref="GeneID:117606399"
ORIGIN      
        1 mvmeaspspq nvgrefvrqy ytllnqapah lhrfynqhss fvhggldsnr estpaigqkq
       61 ihqkiqqlnf rdchakisqv dsqltlengv vvqvsgelsn agqpmrrftq tfvlaiqapk
      121 tyyvhndifr yqdlifpdee etdvggvegv vgesverege egvrseaeee ehqnrgqqlt
      181 apaastepqt qpplipaqqs vlqqqqmyya pppqqshvhp vmqqvlngsv heetpliaqq
      241 qpqqqqqqqq qqqppppppa qqnqyitest sqtefvqete mdvaaepqqp aeseaqpqtn
      301 etqppeadpy assapesepe hqvsntnvts sgpktyanlv ksfpsttgat spqapklsms
      361 pppmtnlrld drsplhptss gpalsassnv stpqqqthrv gnqqpqqqqh qqqraprgga
      421 vqrdgdrrgt rqsqysdahq lflgnlphna seddlrqvfe sygrvvelri hsksndrckg
      481 gaqgnntgkv pnygfitfed qqvvnkvlqn lpiyypaeng qklnveekkv rprmsmdgsg
      541 grlnsndgnm rsmggqqqqq qqrgpvgyfp ggpggvmrgg qhgtrggrgg fsrggdggrg
      601 gggmrqpgnp snpsyqnrr
//
LOCUS       XP_034184660             614 aa            linear   INV 03-SEP-2025
DEFINITION  ras GTPase-activating protein-binding protein 2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184660
VERSION     XP_034184660.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..614
                     /product="ras GTPase-activating protein-binding protein 2
                     isoform X2"
                     /calculated_mol_wt=67471
     Region          8..133
                     /region_name="NTF2"
                     /note="Nuclear transport factor 2 (NTF2) domain plays an
                     important role in the trafficking of macromolecules, ions
                     and small molecules between the cytoplasm and nucleus.
                     This bi-directional transport of macromolecules across the
                     nuclear envelope requires many...; cd00780"
                     /db_xref="CDD:238403"
     Site            order(36,42,50,81,83..84,93,115,126..128,130,132)
                     /site_type="active"
                     /note="TAP/p15 interaction [active]"
                     /db_xref="CDD:238403"
     Site            order(40,42..43,78..79,81,83..84,91,95..97,101,103..106,
                     109,115,126..128,130..133)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238403"
     Site            order(43..44,68,73,96,98,100..101,103,106,129,131,133)
                     /site_type="active"
                     /note="RanGDP-NTF2 interaction [active]"
                     /db_xref="CDD:238403"
     Region          <166..337
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          437..529
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..614
                     /gene="rin"
                     /coded_by="XM_034328769.2:276..2120"
                     /db_xref="GeneID:117606399"
ORIGIN      
        1 mvmeaspspq nvgrefvrqy ytllnqapah lhrfynqhss fvhggldsnr estpaigqkq
       61 ihqkiqqlnf rdchakisqv dsqltlengv vvqvsgelsn agqpmrrftq tfvlaiqapk
      121 tyyvhndifr yqdlifpdee etdvggvegv vgesverege egvrseaeee ehqnrgqqlt
      181 apaastepqt qpplipaqqs vlqqqqmyya pppqqshvhp vmqqvlngsv heetpliaqq
      241 qpqqqqqqqq qqqppppppa qqnqyitest sqtefvqete mdvaaepqqp aeseaqpqtn
      301 etqppeadpy assapesepe hqvsntnvts sgpktyanlv ksfpsttgat spqapklsms
      361 pppmtnlrld drsplhptss gpalsassnv stpqqqthrv gnqqpqqqqh qqqraprgga
      421 vqrdgdrrgt rqsqysdahq lflgnlphna seddlrqvfe sygrvvelri hsksndrckg
      481 gaqgnntgkv pnygfitfed qqvvnkvlqn lpiyypaeng qklnveekkv rprmsmdgsg
      541 grlnsndgnm rsmggqqqqq qqrgpggpgg vmrggqhgtr ggrggfsrgg dggrggggmr
      601 qpgnpsnpsy qnrr
//
LOCUS       XP_034195086             606 aa            linear   INV 03-SEP-2025
DEFINITION  general transcription factor II-I repeat domain-containing protein
            2 [Osmia lignaria lignaria].
ACCESSION   XP_034195086
VERSION     XP_034195086.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339195.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..606
                     /product="general transcription factor II-I repeat
                     domain-containing protein 2"
                     /calculated_mol_wt=69408
     Region          16..70
                     /region_name="DUF4371"
                     /note="Domain of unknown function (DUF4371); cl46273"
                     /db_xref="CDD:480613"
     CDS             1..606
                     /gene="LOC117611258"
                     /coded_by="XM_034339195.2:728..2548"
                     /db_xref="GeneID:117611258"
ORIGIN      
        1 maefhrkrss vstyfkpswe sdyffieqeg vptclicgkk lhqvkkynlq rhfqsmhghy
       61 aqlsgeerea evvqlkqkfv kaeveeetvs scseaavras fgialeiars srsytdgdvv
      121 kkylvnaagq myptevqafe aislsrttia rriqsmasnv mdqlrqlcss ftayslaide
      181 gtdisgtpqi aifirgvnnn lyvteelldl vplrdgvsee dmfscveeii ennqldwhkl
      241 vsiatdgtpa madntnglvg rlrkklnelg nakklvavhc lihrqnlctk niemsnvmsv
      301 ivrvtnyirn hglkrkqfra fleeldseya dlpyytevrw lnrgklleqf ynlrseiaaf
      361 mesmqnpvae lkdndwlvdl afltdlvhhl ntlnqslqrk gmlivelydt iqafqmkiql
      421 wmgqlqigns chfprlkava tnqncftkyc svldlleqef ngrfkdvqsl niefnifatp
      481 fsvnldtvpp emqlelidmk cdrmlknkfn qaeslahfyt gfshirfpll ytlagkilcm
      541 fgstyvceql flnmkktksi hrtgltnynl kcslllsscq sivpditflm eqkrlqtssr
      601 rlftte
//
LOCUS       XP_034195087             604 aa            linear   INV 03-SEP-2025
DEFINITION  triokinase/FMN cyclase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195087
VERSION     XP_034195087.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339196.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195087.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..604
                     /product="triokinase/FMN cyclase isoform X1"
                     /calculated_mol_wt=65571
     Region          26..596
                     /region_name="PRK14479"
                     /note="dihydroxyacetone kinase; Provisional; cl29418"
                     /db_xref="CDD:475197"
     CDS             1..604
                     /gene="LOC117611259"
                     /coded_by="XM_034339196.2:61..1875"
                     /db_xref="GeneID:117611259"
ORIGIN      
        1 mlfpglclll crkvyckkia vhrtmkslvn sindsvtetl yglnyaypql eyqvshkvvl
       61 mprlqdkknk vsvicgggsg hepfaagfvg ngmltasiag sifaappsqh isyaieritk
      121 hnkdgilivi pnytgdclnf giaierarkt glkvaeitvn ddcsipdkeq svagkrglsg
      181 mlfvikiaga laekgsplee vhrtaeivlq ntatyavglt acaipgqppm felpedeiec
      241 gmgvhgevgy erlklkssse vvafmlkhic tslsllsgds vavivnnfga lsqleqgivv
      301 hdvvkhltsm nikplriysg vlvtslnsag ghitllklpe ankkaflsyl deptnapkwp
      361 gcvysipses ippviqdkvl rkiekigiqi dpkqqavlkq ciqsscksli ekeayindld
      421 rgcgdgdcgs tlkrlaadil qnldnfqlsh pssvfhelay taeeqmggts galycllfts
      481 iatelildqk napwthiwar alrvglncli kygkakpgdr smvdvinavc evyegsldkp
      541 pvelckiikt tawkacestk nmkprvgras yvkqeqylqn vdagaygvat wihaitdvik
      601 naki
//
LOCUS       XP_034195088             580 aa            linear   INV 03-SEP-2025
DEFINITION  triokinase/FMN cyclase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195088
VERSION     XP_034195088.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339197.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195088.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="triokinase/FMN cyclase isoform X2"
                     /calculated_mol_wt=62783
     Region          2..572
                     /region_name="PRK14479"
                     /note="dihydroxyacetone kinase; Provisional; cl29418"
                     /db_xref="CDD:475197"
     CDS             1..580
                     /gene="LOC117611259"
                     /coded_by="XM_034339197.2:173..1915"
                     /db_xref="GeneID:117611259"
ORIGIN      
        1 mkslvnsind svtetlygln yaypqleyqv shkvvlmprl qdkknkvsvi cgggsghepf
       61 aagfvgngml tasiagsifa appsqhisya ieritkhnkd gilivipnyt gdclnfgiai
      121 erarktglkv aeitvnddcs ipdkeqsvag krglsgmlfv ikiagalaek gspleevhrt
      181 aeivlqntat yavgltacai pgqppmfelp edeiecgmgv hgevgyerlk lksssevvaf
      241 mlkhictsls llsgdsvavi vnnfgalsql eqgivvhdvv khltsmnikp lriysgvlvt
      301 slnsagghit llklpeankk aflsyldept napkwpgcvy sipsesippv iqdkvlrkie
      361 kigiqidpkq qavlkqciqs scksliekea yindldrgcg dgdcgstlkr laadilqnld
      421 nfqlshpssv fhelaytaee qmggtsgaly cllftsiate lildqknapw thiwaralrv
      481 glnclikygk akpgdrsmvd vinavcevye gsldkppvel ckiikttawk acestknmkp
      541 rvgrasyvkq eqylqnvdag aygvatwiha itdviknaki
//
LOCUS       XP_034195089             512 aa            linear   INV 03-SEP-2025
DEFINITION  triokinase/FMN cyclase isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195089
VERSION     XP_034195089.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195089.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="triokinase/FMN cyclase isoform X3"
                     /calculated_mol_wt=55442
     Region          1..504
                     /region_name="PRK14479"
                     /note="dihydroxyacetone kinase; Provisional; cl29418"
                     /db_xref="CDD:475197"
     CDS             1..512
                     /gene="LOC117611259"
                     /coded_by="XM_034339198.2:153..1691"
                     /db_xref="GeneID:117611259"
ORIGIN      
        1 mltasiagsi faappsqhis yaieritkhn kdgilivipn ytgdclnfgi aierarktgl
       61 kvaeitvndd csipdkeqsv agkrglsgml fvikiagala ekgspleevh rtaeivlqnt
      121 atyavgltac aipgqppmfe lpedeiecgm gvhgevgyer lklksssevv afmlkhicts
      181 lsllsgdsva vivnnfgals qleqgivvhd vvkhltsmni kplriysgvl vtslnsaggh
      241 itllklpean kkaflsylde ptnapkwpgc vysipsesip pviqdkvlrk iekigiqidp
      301 kqqavlkqci qsscksliek eayindldrg cgdgdcgstl krlaadilqn ldnfqlshps
      361 svfhelayta eeqmggtsga lycllftsia telildqkna pwthiwaral rvglncliky
      421 gkakpgdrsm vdvinavcev yegsldkppv elckiiktta wkacestknm kprvgrasyv
      481 kqeqylqnvd agaygvatwi haitdvikna ki
//
LOCUS       XP_034195090             599 aa            linear   INV 03-SEP-2025
DEFINITION  muscarinic Acetylcholine Receptor, A-type isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195090
VERSION     XP_034195090.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339199.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..599
                     /product="muscarinic Acetylcholine Receptor, A-type
                     isoform X1"
                     /calculated_mol_wt=67615
     Region          39..>234
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          41..65
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320428"
     Region          74..96
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320428"
     Region          112..134
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320428"
     Region          157..173
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320428"
     Region          200..223
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320428"
     Region          <508..580
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          513..535
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320428"
     Region          548..573
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320428"
     CDS             1..599
                     /gene="mAChR-A"
                     /coded_by="XM_034339199.2:2169..3968"
                     /db_xref="GeneID:117611261"
ORIGIN      
        1 mnvteepdyy vnksynvtna tnglncsgdp ysyaiwgrvm ivivavflsl ttvvgnimvm
       61 isfkidkqlq tisnyflfsl agadfavgli smplftlytv lgywplgpfi cdtwlaldyl
      121 asnasvlnll lisfdryfsv trpltyrarr ttrkagimia lawaismalw ppwiyawpyi
      181 egkrtvsnna cyiqfietnh litcitaiaa fyvpvtvmis lywriwkete krqkdlpylq
      241 agkpdaskrs nssdealdmd dcrrprsess tgaedinstq lavsylekhy pqyktkyrpl
      301 swmwlkmwci awwhsgrddd dddddiegae ssrtghgyde attplsaetp ltgtvsrsas
      361 lsgihptalt vgksvciadi hdykksgqag elstksissd svytilirlp trdasgmgky
      421 tegpsikmyh deavtfklrs miedveeeng kifrlgstis spstvirrps rmpdiripln
      481 tknipkatkv pankgankkk kkiqekkadr kaaktlsail lafivtwtpy nilvlikslt
      541 acswyipqpv wdfsyylcyi nstvnpvcya lcnaafrrty vrilkckwhn rnrtavdrg
//
LOCUS       XP_076545661             599 aa            linear   INV 03-SEP-2025
DEFINITION  muscarinic Acetylcholine Receptor, A-type isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545661
VERSION     XP_076545661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689546.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..599
                     /product="muscarinic Acetylcholine Receptor, A-type
                     isoform X1"
                     /calculated_mol_wt=67615
     Region          39..>234
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          41..65
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320428"
     Region          74..96
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320428"
     Region          112..134
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320428"
     Region          157..173
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320428"
     Region          200..223
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320428"
     Region          <508..580
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          513..535
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320428"
     Region          548..573
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320428"
     CDS             1..599
                     /gene="mAChR-A"
                     /coded_by="XM_076689546.1:2279..4078"
                     /db_xref="GeneID:117611261"
ORIGIN      
        1 mnvteepdyy vnksynvtna tnglncsgdp ysyaiwgrvm ivivavflsl ttvvgnimvm
       61 isfkidkqlq tisnyflfsl agadfavgli smplftlytv lgywplgpfi cdtwlaldyl
      121 asnasvlnll lisfdryfsv trpltyrarr ttrkagimia lawaismalw ppwiyawpyi
      181 egkrtvsnna cyiqfietnh litcitaiaa fyvpvtvmis lywriwkete krqkdlpylq
      241 agkpdaskrs nssdealdmd dcrrprsess tgaedinstq lavsylekhy pqyktkyrpl
      301 swmwlkmwci awwhsgrddd dddddiegae ssrtghgyde attplsaetp ltgtvsrsas
      361 lsgihptalt vgksvciadi hdykksgqag elstksissd svytilirlp trdasgmgky
      421 tegpsikmyh deavtfklrs miedveeeng kifrlgstis spstvirrps rmpdiripln
      481 tknipkatkv pankgankkk kkiqekkadr kaaktlsail lafivtwtpy nilvlikslt
      541 acswyipqpv wdfsyylcyi nstvnpvcya lcnaafrrty vrilkckwhn rnrtavdrg
//
LOCUS       XP_034195091             599 aa            linear   INV 03-SEP-2025
DEFINITION  muscarinic Acetylcholine Receptor, A-type isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195091
VERSION     XP_034195091.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339200.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..599
                     /product="muscarinic Acetylcholine Receptor, A-type
                     isoform X1"
                     /calculated_mol_wt=67615
     Region          39..>234
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          41..65
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320428"
     Region          74..96
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320428"
     Region          112..134
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320428"
     Region          157..173
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320428"
     Region          200..223
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320428"
     Region          <508..580
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          513..535
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320428"
     Region          548..573
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320428"
     CDS             1..599
                     /gene="mAChR-A"
                     /coded_by="XM_034339200.2:1072..2871"
                     /db_xref="GeneID:117611261"
ORIGIN      
        1 mnvteepdyy vnksynvtna tnglncsgdp ysyaiwgrvm ivivavflsl ttvvgnimvm
       61 isfkidkqlq tisnyflfsl agadfavgli smplftlytv lgywplgpfi cdtwlaldyl
      121 asnasvlnll lisfdryfsv trpltyrarr ttrkagimia lawaismalw ppwiyawpyi
      181 egkrtvsnna cyiqfietnh litcitaiaa fyvpvtvmis lywriwkete krqkdlpylq
      241 agkpdaskrs nssdealdmd dcrrprsess tgaedinstq lavsylekhy pqyktkyrpl
      301 swmwlkmwci awwhsgrddd dddddiegae ssrtghgyde attplsaetp ltgtvsrsas
      361 lsgihptalt vgksvciadi hdykksgqag elstksissd svytilirlp trdasgmgky
      421 tegpsikmyh deavtfklrs miedveeeng kifrlgstis spstvirrps rmpdiripln
      481 tknipkatkv pankgankkk kkiqekkadr kaaktlsail lafivtwtpy nilvlikslt
      541 acswyipqpv wdfsyylcyi nstvnpvcya lcnaafrrty vrilkckwhn rnrtavdrg
//
LOCUS       XP_034195099             598 aa            linear   INV 03-SEP-2025
DEFINITION  muscarinic Acetylcholine Receptor, A-type isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195099
VERSION     XP_034195099.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339208.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..598
                     /product="muscarinic Acetylcholine Receptor, A-type
                     isoform X2"
                     /calculated_mol_wt=67487
     Region          39..>234
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          41..65
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320428"
     Region          74..96
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320428"
     Region          112..134
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320428"
     Region          157..173
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320428"
     Region          200..223
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320428"
     Region          <507..579
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          512..534
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320428"
     Region          547..572
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320428"
     CDS             1..598
                     /gene="mAChR-A"
                     /coded_by="XM_034339208.2:2170..3966"
                     /db_xref="GeneID:117611261"
ORIGIN      
        1 mnvteepdyy vnksynvtna tnglncsgdp ysyaiwgrvm ivivavflsl ttvvgnimvm
       61 isfkidkqlq tisnyflfsl agadfavgli smplftlytv lgywplgpfi cdtwlaldyl
      121 asnasvlnll lisfdryfsv trpltyrarr ttrkagimia lawaismalw ppwiyawpyi
      181 egkrtvsnna cyiqfietnh litcitaiaa fyvpvtvmis lywriwkete krqkdlpylq
      241 agkpdaskrs nssdealdmd dcrrprsess tgaedinstq lavsylekhy pqyktyrpls
      301 wmwlkmwcia wwhsgrdddd ddddiegaes srtghgydea ttplsaetpl tgtvsrsasl
      361 sgihptaltv gksvciadih dykksgqage lstksissds vytilirlpt rdasgmgkyt
      421 egpsikmyhd eavtfklrsm iedveeengk ifrlgstiss pstvirrpsr mpdiriplnt
      481 knipkatkvp ankgankkkk kiqekkadrk aaktlsaill afivtwtpyn ilvlikslta
      541 cswyipqpvw dfsyylcyin stvnpvcyal cnaafrrtyv rilkckwhnr nrtavdrg
//
LOCUS       XP_034195100             402 aa            linear   INV 03-SEP-2025
DEFINITION  TRAF interacting protein no poles [Osmia lignaria lignaria].
ACCESSION   XP_034195100
VERSION     XP_034195100.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339209.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="TRAF interacting protein no poles"
                     /calculated_mol_wt=46615
     Region          4..46
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(5,8,23,25,28,31,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          <64..>265
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..402
                     /gene="nopo"
                     /coded_by="XM_034339209.2:104..1312"
                     /db_xref="GeneID:117611262"
ORIGIN      
        1 mniicaicse slmpsddvfh tpcrhifhfv cltqwlersr scpqcrektt shtihrlyfn
       61 fsnidttsen igtlqdktdk lnfqlllkeq dvkhytekse klgkqnvelk kevhkleael
      121 ktiksvidal kdqikyfkqq nsemeskrqe ieqlrkeiet ykniqilmea stehvdevis
      181 ktcepaalit yicvmkremt vslnkrkelr nklrslqqel arviternll seervkrkkl
      241 eedlmlcene kmclqnkvkq lerkrankfd sdedalssnl kssdgceqnk iktviknaan
      301 eraedtdtkd rhqhtvtkne tnspylplks ggvfvlkhsv qkrsttslhp siltkkarie
      361 ptseqvrana itydgfgghs kherfpsikk kikldpkqks li
//
LOCUS       XP_034195101             337 aa            linear   INV 03-SEP-2025
DEFINITION  activator of 90 kDa heat shock protein ATPase homolog 1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195101
VERSION     XP_034195101.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339210.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="activator of 90 kDa heat shock protein ATPase
                     homolog 1"
                     /calculated_mol_wt=38575
     Region          29..160
                     /region_name="Aha1_N"
                     /note="Activator of Hsp90 ATPase, N-terminal; pfam09229"
                     /db_xref="CDD:462716"
     Region          209..333
                     /region_name="SRPBCC_Aha1"
                     /note="Putative hydrophobic ligand-binding SRPBCC domain
                     of the Hsp90 co-chaperone Aha1 and related proteins;
                     cd08892"
                     /db_xref="CDD:176901"
     Site            order(210,212,214,223..225,227..228,233,241,249,251,253,
                     255,257,259..260,262,265,270,272..273,286..287,289,291,
                     301,303,305,307,311..319,322..324,327)
                     /site_type="other"
                     /note="putative hydrophobic ligand binding site [chemical
                     binding]"
                     /db_xref="CDD:176901"
     Site            order(262,273,286,296..297)
                     /site_type="other"
                     /note="putative Hsp90 binding residues [polypeptide
                     binding]"
                     /db_xref="CDD:176901"
     CDS             1..337
                     /gene="LOC117611263"
                     /coded_by="XM_034339210.2:335..1348"
                     /db_xref="GeneID:117611263"
ORIGIN      
        1 makwgegdpr wiveerpdat nvnnwhwtek nacawsqekl kelftnmkie gdgvsctvte
       61 tekcegeata nnrkgkliff yewnivlkwv segksnkmie gkinipnlse endisevdie
      121 vtlkdstdeg eavkhflhtk gkeaireklk qyvsslkeef tvgmilpkkd nvkenissit
      181 sgfnakmqmn stavstnkke vgckisttti kqqqkfqcra eefynvfttt emvqaftkgp
      241 vkldlkkagk felfggnihg dfveitptki vqkwrckqwp dghfsdvtid isekndhtev
      301 nltqigvpvs eeestkenwe ryywdaikrt fgfgyfm
//
LOCUS       XP_034195102             337 aa            linear   INV 03-SEP-2025
DEFINITION  activator of 90 kDa heat shock protein ATPase homolog 1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195102
VERSION     XP_034195102.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339211.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="activator of 90 kDa heat shock protein ATPase
                     homolog 1"
                     /calculated_mol_wt=38575
     Region          29..160
                     /region_name="Aha1_N"
                     /note="Activator of Hsp90 ATPase, N-terminal; pfam09229"
                     /db_xref="CDD:462716"
     Region          209..333
                     /region_name="SRPBCC_Aha1"
                     /note="Putative hydrophobic ligand-binding SRPBCC domain
                     of the Hsp90 co-chaperone Aha1 and related proteins;
                     cd08892"
                     /db_xref="CDD:176901"
     Site            order(210,212,214,223..225,227..228,233,241,249,251,253,
                     255,257,259..260,262,265,270,272..273,286..287,289,291,
                     301,303,305,307,311..319,322..324,327)
                     /site_type="other"
                     /note="putative hydrophobic ligand binding site [chemical
                     binding]"
                     /db_xref="CDD:176901"
     Site            order(262,273,286,296..297)
                     /site_type="other"
                     /note="putative Hsp90 binding residues [polypeptide
                     binding]"
                     /db_xref="CDD:176901"
     CDS             1..337
                     /gene="LOC117611263"
                     /coded_by="XM_034339211.2:317..1330"
                     /db_xref="GeneID:117611263"
ORIGIN      
        1 makwgegdpr wiveerpdat nvnnwhwtek nacawsqekl kelftnmkie gdgvsctvte
       61 tekcegeata nnrkgkliff yewnivlkwv segksnkmie gkinipnlse endisevdie
      121 vtlkdstdeg eavkhflhtk gkeaireklk qyvsslkeef tvgmilpkkd nvkenissit
      181 sgfnakmqmn stavstnkke vgckisttti kqqqkfqcra eefynvfttt emvqaftkgp
      241 vkldlkkagk felfggnihg dfveitptki vqkwrckqwp dghfsdvtid isekndhtev
      301 nltqigvpvs eeestkenwe ryywdaikrt fgfgyfm
//
LOCUS       XP_076545662             306 aa            linear   INV 03-SEP-2025
DEFINITION  secreted protein, acidic, cysteine-rich [Osmia lignaria lignaria].
ACCESSION   XP_076545662
VERSION     XP_076545662.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689547.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..306
                     /product="secreted protein, acidic, cysteine-rich"
                     /calculated_mol_wt=35516
     Region          85..170
                     /region_name="FSL_SPARC"
                     /note="Follistatin-like SPARC (secreted protein, acidic,
                     and rich in cysteines) domain; SPARC/BM-40/osteonectin is
                     a multifunctional glycoprotein which modulates cellular
                     interaction with the extracellular matrix by its binding
                     to structural matrix proteins...; cd01328"
                     /db_xref="CDD:238649"
     Site            128
                     /site_type="other"
                     /note="N-glycosylation site [posttranslational
                     modification]"
                     /db_xref="CDD:238649"
     Region          173..288
                     /region_name="EFh_SPARC_like"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted protein acidic and rich in cysteine
                     (SPARC) and similar proteins; cd16231"
                     /db_xref="CDD:320010"
     Site            order(180..181,184..185,187..188,191..192,195,256..257)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:320010"
     Region          221..254
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320010"
     Site            order(233,238,245,268,270,272,279)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320010"
     Site            order(243..244,258)
                     /site_type="other"
                     /note="EC/FS-domain interface"
                     /db_xref="CDD:320010"
     Region          256..288
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320010"
     CDS             1..306
                     /gene="SPARC"
                     /coded_by="XM_076689547.1:1854..2774"
                     /db_xref="GeneID:117606400"
ORIGIN      
        1 mgtklklllf allmvslmvd vsahqrrrkh rrhtttteas aqdevmnmle adeiaeeaae
       61 naevtvedgr nevgsksqsl lrmdpcmskh cgagrvckai deetaecvcv eicdeendpr
      121 rkvctnynet fhsdcevyqq rcfcdsnddr crgpdyqhvh ieyygecrqm pmckeeemad
      181 fprrmrdwlf nimrdladrq elpshylkmq reaetnhtlr wsnaaiwkwc dldghphdra
      241 vsrhelfpir aplmalehci gpfldscdin ddhkitliew gkclqleedd ldekcdqlve
      301 aqnsdq
//
LOCUS       XP_076545663             306 aa            linear   INV 03-SEP-2025
DEFINITION  secreted protein, acidic, cysteine-rich [Osmia lignaria lignaria].
ACCESSION   XP_076545663
VERSION     XP_076545663.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689548.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..306
                     /product="secreted protein, acidic, cysteine-rich"
                     /calculated_mol_wt=35516
     Region          85..170
                     /region_name="FSL_SPARC"
                     /note="Follistatin-like SPARC (secreted protein, acidic,
                     and rich in cysteines) domain; SPARC/BM-40/osteonectin is
                     a multifunctional glycoprotein which modulates cellular
                     interaction with the extracellular matrix by its binding
                     to structural matrix proteins...; cd01328"
                     /db_xref="CDD:238649"
     Site            128
                     /site_type="other"
                     /note="N-glycosylation site [posttranslational
                     modification]"
                     /db_xref="CDD:238649"
     Region          173..288
                     /region_name="EFh_SPARC_like"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted protein acidic and rich in cysteine
                     (SPARC) and similar proteins; cd16231"
                     /db_xref="CDD:320010"
     Site            order(180..181,184..185,187..188,191..192,195,256..257)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:320010"
     Region          221..254
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320010"
     Site            order(233,238,245,268,270,272,279)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320010"
     Site            order(243..244,258)
                     /site_type="other"
                     /note="EC/FS-domain interface"
                     /db_xref="CDD:320010"
     Region          256..288
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320010"
     CDS             1..306
                     /gene="SPARC"
                     /coded_by="XM_076689548.1:77..997"
                     /db_xref="GeneID:117606400"
ORIGIN      
        1 mgtklklllf allmvslmvd vsahqrrrkh rrhtttteas aqdevmnmle adeiaeeaae
       61 naevtvedgr nevgsksqsl lrmdpcmskh cgagrvckai deetaecvcv eicdeendpr
      121 rkvctnynet fhsdcevyqq rcfcdsnddr crgpdyqhvh ieyygecrqm pmckeeemad
      181 fprrmrdwlf nimrdladrq elpshylkmq reaetnhtlr wsnaaiwkwc dldghphdra
      241 vsrhelfpir aplmalehci gpfldscdin ddhkitliew gkclqleedd ldekcdqlve
      301 aqnsdq
//
LOCUS       XP_076545664             169 aa            linear   INV 03-SEP-2025
DEFINITION  histone H2A variant [Osmia lignaria lignaria].
ACCESSION   XP_076545664
VERSION     XP_076545664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689549.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..169
                     /product="histone H2A variant"
                     /calculated_mol_wt=17907
     Region          47..162
                     /region_name="PLN00154"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:177756"
     CDS             1..169
                     /gene="His2Av"
                     /coded_by="XM_076689549.1:16..525"
                     /db_xref="GeneID:143305524"
ORIGIN      
        1 mvrlrdapta psvfggtair rrrlcfsqvc rvanrkdscq imaggkagkd sgkakakavs
       61 rsaraglqfp vgrihrhlkn rttshgrvga taavysaail eyltaevlel agnaskdlkv
      121 kritprhlql airgdeelds likatiaggg viphihksli gkkgsqkpa
//
LOCUS       XP_034185050             314 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606573 [Osmia lignaria lignaria].
ACCESSION   XP_034185050
VERSION     XP_034185050.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329159.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..314
                     /product="uncharacterized protein LOC117606573"
                     /calculated_mol_wt=31153
     Region          184..236
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..314
                     /gene="LOC117606573"
                     /coded_by="XM_034329159.2:169..1113"
                     /db_xref="GeneID:117606573"
ORIGIN      
        1 mrssppptpt dghsntyrli hgyaalrinc nkgsgwyins svdhllsslr vfahnsnmny
       61 hqlmkallvl sclasnalag llpggsaggs gyqynrpggg gfggpgglpg dlsggrggdf
      121 ggslrgrpna lygapgaggd ggfggrpsta ygapgqfggg gggggyqggs gggyggdngr
      181 pqpysfqyev ydppsgndys qressdgnvv qgeyrvllpd srtqivkyma ddangytadv
      241 qyegqaqfsr ggaggggypg gaggypggag gyqggaggag gaggyqggag rgvygapada
      301 gsnqylpprn nyrk
//
LOCUS       XP_076545665             156 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609735 [Osmia lignaria lignaria].
ACCESSION   XP_076545665
VERSION     XP_076545665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689550.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..156
                     /product="uncharacterized protein LOC117609735"
                     /calculated_mol_wt=18347
     CDS             1..156
                     /gene="LOC117609735"
                     /coded_by="XM_076689550.1:1125..1595"
                     /db_xref="GeneID:117609735"
ORIGIN      
        1 mlldnifafn eievseessl dfldqfkece vesveehdda denwsgethi gvcsqrdedv
       61 dvdykrkava fwtegkkrrk latvqhnfrl vsserqlrrw ahkietsgtk kerlaqisef
      121 vitnmkkaie sgyilhdrni rrwavqayke qgnrrr
//
LOCUS       XP_034194876             113 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611088 [Osmia lignaria lignaria].
ACCESSION   XP_034194876
VERSION     XP_034194876.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338985.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..113
                     /product="uncharacterized protein LOC117611088"
                     /calculated_mol_wt=12808
     CDS             1..113
                     /gene="LOC117611088"
                     /coded_by="XM_034338985.2:1185..1526"
                     /db_xref="GeneID:117611088"
ORIGIN      
        1 metmqehllp aideteepds pilnenhhmy ingeelpeps pcqkifvnsq klneaiseav
       61 gaaisreink ihqeaiwqma aatrmlrplt ppsrprtrrp rkrptpppte fth
//
LOCUS       XP_034190272             196 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608783 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190272
VERSION     XP_034190272.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334381.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190272.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="uncharacterized protein LOC117608783 isoform X1"
                     /calculated_mol_wt=23828
     CDS             1..196
                     /gene="LOC117608783"
                     /coded_by="XM_034334381.2:370..960"
                     /db_xref="GeneID:117608783"
ORIGIN      
        1 mrysvsnead ranllwaveq rpqlqlkmrq rrmqiniqrq pklyplhagy tphqhqphph
       61 pcpqhaeytp hqhqlqpyqp lpypyphqpy pypyepypcp yqtqpyqpqp yqthpyqpqp
      121 yqthpyqpqp qpyqqqaedt tyrsmqqrel rrcrrelkrl qretmprrgr rnctnrpvnq
      181 ycgachkpvp gcrryi
//
LOCUS       XP_034190273             195 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608783 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190273
VERSION     XP_034190273.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334382.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190273.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..195
                     /product="uncharacterized protein LOC117608783 isoform X2"
                     /calculated_mol_wt=23724
     CDS             1..195
                     /gene="LOC117608783"
                     /coded_by="XM_034334382.2:118..705"
                     /db_xref="GeneID:117608783"
ORIGIN      
        1 mdmrsleadr anllwaveqr pqlqlkmrqr rmqiniqrqp klyplhagyt phqhqphphp
       61 cpqhaeytph qhqlqpyqpl pypyphqpyp ypyepypcpy qtqpyqpqpy qthpyqpqpy
      121 qthpyqpqpq pyqqqaedtt yrsmqqrelr rcrrelkrlq retmprrgrr nctnrpvnqy
      181 cgachkpvpg crryi
//
LOCUS       XP_034185421            2698 aa            linear   INV 03-SEP-2025
DEFINITION  putative aminopeptidase-2 [Osmia lignaria lignaria].
ACCESSION   XP_034185421
VERSION     XP_034185421.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329530.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185421.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2698
                     /product="putative aminopeptidase-2"
                     /calculated_mol_wt=312586
     Region          51..489
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(169,171,299..302,334..335,338,357,361,415,420)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          565..889
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     Region          930..1370
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(1050,1052,1183..1186,1218..1219,1222,1241,1245,1299,
                     1304)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          1443..1745
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     Region          1801..2247
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(1921,1923,2055..2058,2091..2092,2095,2114,2118,2172,
                     2177)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          2317..2624
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..2698
                     /gene="LOC117606718"
                     /coded_by="XM_034329530.2:341..8437"
                     /db_xref="GeneID:117606718"
ORIGIN      
        1 mnfiqdwasi tvlllvvagf qltkgnqepf vedgenitfy dyrlpksvip isyeinlipt
       61 mggdfifigs msinaivqql tdtvtlhrgs vnittltvnw nnqtinktps ryndttekye
      121 islnrtlnkg ekisiniayn gklrddmigf ykssyidsng kirwmattqf qtaharhafp
      181 cfdepsfkar ftirilrppg ynslsnmplh ksykepqgtw defqqsipms tylvafiisd
      241 feslqlgnls iwarpnairq amyardlsld atiylsrlfk qdfnipkmdm vaipdfsaga
      301 menwgiityr emrllcdsst sdyanqsvat vivhelthew fgnmitpeww sylwlseafa
      361 ryfqyfgtaq iektwnleeq fvveqhqtay asdgvessqp msrevsntqq lssigdtity
      421 nkggsivrmm nlifgsdvfe aslqnylsnn ketkvarpen lweefqkein krnqslnatv
      481 reimstwtek agypvlniai nregvaslqq rrfflrnlks tptnltwwvp lswttkenpn
      541 fnvvkprywl nkerdtinvg nssgwvifnv qssgfyrvny dyaswyrife tlnsknyadi
      601 hvlnravlvd dslnlaragm lsynitfdcl qylkfetnyl pfksafsgls yldqrlsgha
      661 eyykqfknfv lvlienryke lgyldrpndd rltvllrgel nkwacnyghq gciqtftnmf
      721 qqwrknntst ikpnqrsvay cmgvkygskd dweflwnqyf nsnsandqit llqalgcsnn
      781 stileiylls alksfeetri rkqdctsvfs avygsgigga efvlnfvdky haqmkeyngl
      841 glvssvlssa sqrfstkqlv dkfdnliqrr kvefesiqts lqnaleiaky elwwfeknid
      901 piiswidshd kenpppidse yrlpqnlsps kyyidvktdv nqadnftfdg tvridtvvkq
      961 ktktivlhss dlshdnvnvl vgdksvavls ftvdkkydfl tievaqelqv gqnvtivvgf
     1021 kgslnedmrg fyrswyfdly gkvrwlaath mepvgarkmf pcfdepamka kfvvnvtvpg
     1081 dysaisnmpi ehtvdhgngr rsllfketpi mstylvalvv sdfkvlrntn gkyavwtnpm
     1141 aikyanysls vmeplvsyye kalsipyqlp kldmvalpdf vsgamenwgl ltykerylle
     1201 gdesttdskq siidvishei thqwfgdyvs plwwkylwln egfaryfqyh gtasvkkdws
     1261 leaqfvveql hsaletdsla sthpmthdvy sptqirgifd tisyakaasv irmlekslgn
     1321 dvffkalhny lekrkydvat ptdladafkd qitdpvvkne ihsimnswtn qsgypvihvt
     1381 vqpdyvilrq erfilkpgkd kvsaakwytp iswtslntln fndtkprywl qnttdslpnk
     1441 ndlillnlqq sgfyrtnydn qtwkriinvl ksdqrekihe inrasliddl mnlgraemvd
     1501 ydivfsatqy ltqetdyipw raffnnlnyl hkhmqerday gafkryassl llpiynklgf
     1561 edketdshvt klfrshvrkw acklnisnce eeawsywrfn pssnkyasiq anyysvvyct
     1621 varrnsfsdw dllmdlykit sfpahkltll qslacttdkv lldqllhqai tkdyvirfed
     1681 sssvfsyvmd aspegvetvi efvknnyekm vkyfrqessv rsivsaigkk vftqklynky
     1741 tnllewlsvk dptsqntlns ykndpdyelk waerhipmlh ewfetkyshe dyrlpstfsp
     1801 lkynislspg feernftftg rvkieierkr ddvsrivvna yklyikdvsv yqnpseplqv
     1861 sswllnpntq tlsifmknfi ksdklfvdit ytgnlnddme gfyrshyvns kkqlrwlatt
     1921 qfeptyarqa fpcfdepamk akfviemerp tdyvalsnmp lsstrpsnnt grvwdtfket
     1981 lnmssyliaf vvcdfqklrt slqstnvwgr peivakgela eivaprilqy lfeetehlft
     2041 fpkldligip dfqmgamenw glvtfreygl fyekdvttak ytdyiitiia helahsmfgn
     2101 lvtcdwweyi wlnegfaeym qwrlsdmyqp qynyqhlvvv ndlqpallnd asssthpmnn
     2161 pvskpseikk vfdtvtygks ssvirmiqkt fgqevfknvi snyltrhrys tatpkdlwkh
     2221 ftrevnktgg lgewnitfes lmdgwtnkpg ypivlaeynf ptlkltqkhy stykrsgdfw
     2281 ipitmtdsyt pdfmktstnl wlgedilrln lkrdvdwfii niqqsgyyrv nydtdswlrl
     2341 ikalmepths gihftnraqi vddafnlard ghltynitig ttvylvkekn ylpwkaffna
     2401 lnfivqryeg rigsdllrdy vlalttnryk dlgfldsyte phlnqlnrel iltwtckygn
     2461 kdcvtqstsl frewrkdvkn kngispnara avyctalksg sdtewnflwe qylktdlate
     2521 kmvmlnafgc tdnkillnky iqymldrtyp skirkqdisa afasiynagq hgvdaildfv
     2581 mndyeklyey ygnwdgvadl iskvasrvst ssqilklntf aetkvgnitn llpavqsaik
     2641 takknedwyl tnspvinrll khaitaiqkh nssaavtiqp llilfttvct ifvyilsn
//
LOCUS       XP_034185647             983 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase Ey [Osmia lignaria lignaria].
ACCESSION   XP_034185647
VERSION     XP_034185647.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329756.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185647.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..983
                     /product="aminopeptidase Ey"
                     /calculated_mol_wt=112961
     Region          101..552
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(225,227,361..364,397..398,401,420,424,478,483)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          635..962
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..983
                     /gene="LOC117606818"
                     /coded_by="XM_034329756.2:236..3187"
                     /db_xref="GeneID:117606818"
ORIGIN      
        1 mtqsrevlam mdahtttfgr krgctvspcg aflltaafli slvvtgllvy hlapcleekd
       61 akacndlsna vfesrgifst dtsnkqkldv rlprsivpdl yelrlipfiw egnftfngev
      121 kvlvnvtedt rnitlhavdm midvdftnik eysatsnnsn tvrirgqknd terqfhvirt
      181 sdtlkrgkqy vlhlkfvgyl kdylqgfyrs sytidgqtrw iattqfqptd arrafpcfde
      241 palkarfqis iarprnmtsi snmprkgepm pvpglpsymw dhyersvpms tylvafivsd
      301 fevlksesgn frvwarreai eqaqyclkig pkileyyedy fkiefplpki dnvalpdfsa
      361 gamenwglit yretamlyqe gvstssnqqr vatvvshela hqwfgnlvtp swwtdlwlne
      421 gfasyveyig mnaveptwkv leqfvvhdlq nvfgldales shpisikvgh pdeiseifdk
      481 isygkgasii rmmdhfltte vfkqgltnyl nakayqsaeq ndlwdaltkq ahkdkvlhss
      541 itikkimdtw tlqtgfpvvt vtrnynngai tltqerfllr ngttmvitds eplwwipity
      601 tterqldfst trpsqwmkae ksitlsdldl spsewlifni qetgyyrvny dranwqmiik
      661 qlnkdsfrni stinraqlid dalnlaragk ldyataldvt sylaheteyl pwkaaftamh
      721 ylddmlikms sydkfriyvl klldnvykqv gfkdnpgdpq ltvftridvl twacnfghed
      781 ciknagkqfy nwrntpnpnq nnpispnlks vvyctairvg gqtewdfawr ryletnvgse
      841 kdlllhalgc tretwllsry ldwaitensg irkqdvgrvl nsvacnaigq plafnffrde
      901 warlreyfgt slltinniik satraintky elkdlldftt ehkqelgsat rtiqqaieqs
      961 eanirwvnas hsiiydwlkr nta
//
LOCUS       XP_034185648             983 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase Ey [Osmia lignaria lignaria].
ACCESSION   XP_034185648
VERSION     XP_034185648.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329757.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185648.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..983
                     /product="aminopeptidase Ey"
                     /calculated_mol_wt=112961
     Region          101..552
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(225,227,361..364,397..398,401,420,424,478,483)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          635..962
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..983
                     /gene="LOC117606818"
                     /coded_by="XM_034329757.2:213..3164"
                     /db_xref="GeneID:117606818"
ORIGIN      
        1 mtqsrevlam mdahtttfgr krgctvspcg aflltaafli slvvtgllvy hlapcleekd
       61 akacndlsna vfesrgifst dtsnkqkldv rlprsivpdl yelrlipfiw egnftfngev
      121 kvlvnvtedt rnitlhavdm midvdftnik eysatsnnsn tvrirgqknd terqfhvirt
      181 sdtlkrgkqy vlhlkfvgyl kdylqgfyrs sytidgqtrw iattqfqptd arrafpcfde
      241 palkarfqis iarprnmtsi snmprkgepm pvpglpsymw dhyersvpms tylvafivsd
      301 fevlksesgn frvwarreai eqaqyclkig pkileyyedy fkiefplpki dnvalpdfsa
      361 gamenwglit yretamlyqe gvstssnqqr vatvvshela hqwfgnlvtp swwtdlwlne
      421 gfasyveyig mnaveptwkv leqfvvhdlq nvfgldales shpisikvgh pdeiseifdk
      481 isygkgasii rmmdhfltte vfkqgltnyl nakayqsaeq ndlwdaltkq ahkdkvlhss
      541 itikkimdtw tlqtgfpvvt vtrnynngai tltqerfllr ngttmvitds eplwwipity
      601 tterqldfst trpsqwmkae ksitlsdldl spsewlifni qetgyyrvny dranwqmiik
      661 qlnkdsfrni stinraqlid dalnlaragk ldyataldvt sylaheteyl pwkaaftamh
      721 ylddmlikms sydkfriyvl klldnvykqv gfkdnpgdpq ltvftridvl twacnfghed
      781 ciknagkqfy nwrntpnpnq nnpispnlks vvyctairvg gqtewdfawr ryletnvgse
      841 kdlllhalgc tretwllsry ldwaitensg irkqdvgrvl nsvacnaigq plafnffrde
      901 warlreyfgt slltinniik satraintky elkdlldftt ehkqelgsat rtiqqaieqs
      961 eanirwvnas hsiiydwlkr nta
//
LOCUS       XP_034185422             925 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase N-like [Osmia lignaria lignaria].
ACCESSION   XP_034185422
VERSION     XP_034185422.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329531.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185422.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="aminopeptidase N-like"
                     /calculated_mol_wt=105962
     Region          39..487
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(161,163,297..300,333..334,337,356,360,415,420)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          561..863
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..925
                     /gene="LOC117606719"
                     /coded_by="XM_034329531.2:172..2949"
                     /db_xref="GeneID:117606719"
ORIGIN      
        1 msrvlsfvll lvtcnvaygd vassqaeiny rlpndvsvin yelsmepdlt nfiftgqvki
       61 tikilndins ftlnakhlnv tkenvtvtae dpkdpavtat evdhqeepef lvihlsgkpa
      121 ktknynllik ftgelhnntk gfyrsryfdk dnnvkyiatt hfeptgarwa fpcwdepeyk
      181 apftitiths dkytaiantp lvgepkkieg nklvstfeks ppmstylvaf vvsdyecleg
      241 sdksfrvctk shakehgkya leigqkvigk lneytglnys syvrkmdqvt ikdfspsame
      301 nwglvtyret sllyeegvtt trskqsiatv iahefahqwf gnlvspkwwq yiwlnegfat
      361 ymqyfithel ipewrlnetf vvtniqgnaf iadavqnvrh mnkdaqspse isslfdsigy
      421 qkaasvirmm shiltntvfq nglkgylnky qktaatsndl laalqnaagd ktwgganfse
      481 imnewvnkpg fpvvnvkkvk dgefevsqkr flsdgstdet kwwvpltyth kselefkntt
      541 pvawlkangd svtinidtke wilfnklhsg yyrvnydeen wkkldsylkt ndyanippin
      601 raqliddvfs laqadyldyt talsltnyln kevdyvpwss tirnlnflnn mmrnskryyi
      661 fkkyiefitk nvvkevgfev kkdeselhki lrtdlikwac kagiekctky ttekynlwfe
      721 dstknrldvd lrntilcagv rnvdfdkwkk tldkllesak dedekrslml nvpgcsnsve
      781 vlkkylnltl eenppvsfdv avqgivseha dgvnitletl ltqqerikkl pnvdsvirts
      841 vqaianyikt rdeyvelskf vakeleakhv qdmvlkaskn mkwlesneev iekwllsnen
      901 lfnsatsltf asllvllsif itrfy
//
LOCUS       XP_034185423             914 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase N [Osmia lignaria lignaria].
ACCESSION   XP_034185423
VERSION     XP_034185423.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185423.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..914
                     /product="aminopeptidase N"
                     /calculated_mol_wt=104798
     Region          37..489
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(161,163,297..300,333..334,337,356,360,415,420)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          566..891
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..914
                     /gene="LOC117606720"
                     /coded_by="XM_034329532.2:240..2984"
                     /db_xref="GeneID:117606720"
ORIGIN      
        1 merglfnilf litfsinvck sdidhasyrl peevvpisyd lwlftrlgyn dfnykgyvni
       61 slnvlnktdv ivvhndglli qeessilyrv tselnesivp ianqssdter qfyilkfssk
      121 levgvyhlvl yfegevqdgv fgfyrssyvd ntktkwmgit qfsptyarqa fpcmdephlk
      181 asfilhight gnqtttsntv pqstikhsnn enyyvttfap tpkmstylvg wsvhdfvpet
      241 stfsnnlkiw trrsmnhhgs islyqslsiy tflrkyllie npiskldeia vpdfhfname
      301 nwgmitfres valyeggvta tkkmvdgftt maheyahtwf gnlvthrfwd vvwlkegfas
      361 yfqyfalskt qpswgmmnkf vvdvlqptll gdsenhtrtm ngrnvgspss imammdfvsy
      421 kkgasiirml shvmgdlafq eglqsylknm sykaatpsdl yqhlqaslnn snqleggvli
      481 kdfmeswtnk pgfplvtvtr tynnslvlid hqpfyqypss knrsdwwipl nfatksindf
      541 sntgpsywls peetlilfgt aspndwiifn iqqtgyyrvn ydernwkmli dylksenyrk
      601 ihkvnraali ddafnlarag ylnysipfnl snyliqetdy epwvaavnsf kflnkmlssl
      661 pdmqqsfqeh vtqllqpmye qlsfneslne dittklsrel ilstsclvgn ihclttseml
      721 frswiaspnk lipndlksfv ycegiragdy edwytllkrw qstdlhteye vllqalgctr
      781 nttlineylq lsisepnvrk qyrftiisai idgntrnvdy vlalveknvq tiikrrghdf
      841 lsktitaign aittaeqvsk lhsfvdghyn elgsalkgak qaitkasetv ewiknvlpsi
      901 eahfnsnttm sgsf
//
LOCUS       XP_034185424             914 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase N [Osmia lignaria lignaria].
ACCESSION   XP_034185424
VERSION     XP_034185424.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329533.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185424.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..914
                     /product="aminopeptidase N"
                     /calculated_mol_wt=104798
     Region          37..489
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(161,163,297..300,333..334,337,356,360,415,420)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          566..891
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..914
                     /gene="LOC117606720"
                     /coded_by="XM_034329533.2:240..2984"
                     /db_xref="GeneID:117606720"
ORIGIN      
        1 merglfnilf litfsinvck sdidhasyrl peevvpisyd lwlftrlgyn dfnykgyvni
       61 slnvlnktdv ivvhndglli qeessilyrv tselnesivp ianqssdter qfyilkfssk
      121 levgvyhlvl yfegevqdgv fgfyrssyvd ntktkwmgit qfsptyarqa fpcmdephlk
      181 asfilhight gnqtttsntv pqstikhsnn enyyvttfap tpkmstylvg wsvhdfvpet
      241 stfsnnlkiw trrsmnhhgs islyqslsiy tflrkyllie npiskldeia vpdfhfname
      301 nwgmitfres valyeggvta tkkmvdgftt maheyahtwf gnlvthrfwd vvwlkegfas
      361 yfqyfalskt qpswgmmnkf vvdvlqptll gdsenhtrtm ngrnvgspss imammdfvsy
      421 kkgasiirml shvmgdlafq eglqsylknm sykaatpsdl yqhlqaslnn snqleggvli
      481 kdfmeswtnk pgfplvtvtr tynnslvlid hqpfyqypss knrsdwwipl nfatksindf
      541 sntgpsywls peetlilfgt aspndwiifn iqqtgyyrvn ydernwkmli dylksenyrk
      601 ihkvnraali ddafnlarag ylnysipfnl snyliqetdy epwvaavnsf kflnkmlssl
      661 pdmqqsfqeh vtqllqpmye qlsfneslne dittklsrel ilstsclvgn ihclttseml
      721 frswiaspnk lipndlksfv ycegiragdy edwytllkrw qstdlhteye vllqalgctr
      781 nttlineylq lsisepnvrk qyrftiisai idgntrnvdy vlalveknvq tiikrrghdf
      841 lsktitaign aittaeqvsk lhsfvdghyn elgsalkgak qaitkasetv ewiknvlpsi
      901 eahfnsnttm sgsf
//
LOCUS       XP_034185425             904 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase N-like [Osmia lignaria lignaria].
ACCESSION   XP_034185425
VERSION     XP_034185425.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329534.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185425.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..904
                     /product="aminopeptidase N-like"
                     /calculated_mol_wt=105429
     Region          48..494
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(168,170,303..306,339..340,343,362,366,420,425)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          581..837
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..904
                     /gene="LOC117606721"
                     /coded_by="XM_034329534.2:292..3006"
                     /db_xref="GeneID:117606721"
ORIGIN      
        1 mmayakiiai svlvcaisal pvqnddpgtn pnnetkinyr lpnstapvhy dlkiipdidg
       61 dsgfkgeiii hfkvleptsd ialhsvrlnv denatrviys dgivlrpeah tyiettdqli
      121 isvdrileeg eywmtmrfsg hykeglhgff kgyytneegn dvwlvathfe ptharyafpc
      181 wdepalkatf nisikhydny talsnmpvre ktnvdeadgk rwthfektpv mstylvayvv
      241 adfekisnad gsiniwsrke lvphlkyale iaekatrvle qytnstvrvp kmdhvalpqh
      301 gsgamenwgl itypeinlaf desvnslerk raiaktvahe lahqwfgnkv spiwwshiwl
      361 negfasyfqh ytlnkifknw reidyfiiss lqdslvtdtq whtnpidvdl etpeqiksaf
      421 siaiykkaps ilhmlsniis pevfqkgvvd yldrheydsa dpdclwralq saldksdvph
      481 sdfnvkevmd twikqkrypl vtvernystg emklrqedfa lvdvhmhans teykdeketh
      541 qwwvpityat esnpdfsdig sikwlkpnev ltvdgidpdd wiivnkqqsg yyrvnydean
      601 wrkiakylrs enytnihlls raqivsditr lvfydrvkps vfldlivylk qeqdytqwih
      661 vfdllddikl vldvplaqpi rdfilslmen lmktvgyddn dnedpltrfk rhkalewacy
      721 lghteckema tirliqyind rdiykkspgl aewavtygpq lanesvwhqi wefyleekmi
      781 yrlsvtedpt lfqaylniti sndvslwyrg mlveaamkhr lfsnvamdfc iehwdqlsgs
      841 sghnyhcide mrmiimdeer iekvkkicen isdcnwekfw pkyekyvkrt kseiekwiaa
      901 indl
//
LOCUS       XP_034185653             867 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185653
VERSION     XP_034185653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329762.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..867
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X1"
                     /calculated_mol_wt=98836
     Region          27..856
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..867
                     /gene="Vha100-2"
                     /coded_by="XM_034329762.2:192..2795"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff tenqdsylll yqqeeandsi trtlineeaq nssaigrgrl
      181 efvagvinre rvpafermlw risrgnvflr qaeldrpled patsenvmsv tageqrysrh
      241 sgnkmyktaf vaffqgeqlk srikkvcsgf haslypcphs haervdmvkg vrtrledlnl
      301 vlnqthdhrq rvlhnvakel pnwmimvrkm kaiyhtmnlf nmdvskkcli gecwvpvadl
      361 givrnclteg srlcgssips flnviytden pptfnrtnkf trgfqnlida ygvasyrean
      421 palytiitfp flfsimfgdc ghglimtlfa lymiilekkl maqkstseiw niffagryii
      481 llmglfsiyt giiyndlfsk sfnifgssws irynkstime nnvlqlnpgt dayaqvpypm
      541 gldpvwmlae nkiiflnsyk mklsiifgvv hmifgvcvsi vnilnfkkys slfleflpql
      601 lflillffyl vllmfvkwil ydasstdvvy spgcapsvli tfinmilmgh tvipgcpeym
      661 fpgqqilqqv cvalaalcip vmlfgrpvyy lctknkkkad rvwsngstsq dielqteglq
      721 tdgpsnpeaa gnhdqenesf geimihqaih tieyvlstis htasylrlwa lslahgqlse
      781 vlwtmvlrag lgakdgsyvy sgllfvsfaa wafftlailv mmeglsaflh tlrlhwvefm
      841 skfydgqgya fqpfcfknil dtedlee
//
LOCUS       XP_034185651             867 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185651
VERSION     XP_034185651.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..867
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X1"
                     /calculated_mol_wt=98836
     Region          27..856
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..867
                     /gene="Vha100-2"
                     /coded_by="XM_034329760.2:199..2802"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff tenqdsylll yqqeeandsi trtlineeaq nssaigrgrl
      181 efvagvinre rvpafermlw risrgnvflr qaeldrpled patsenvmsv tageqrysrh
      241 sgnkmyktaf vaffqgeqlk srikkvcsgf haslypcphs haervdmvkg vrtrledlnl
      301 vlnqthdhrq rvlhnvakel pnwmimvrkm kaiyhtmnlf nmdvskkcli gecwvpvadl
      361 givrnclteg srlcgssips flnviytden pptfnrtnkf trgfqnlida ygvasyrean
      421 palytiitfp flfsimfgdc ghglimtlfa lymiilekkl maqkstseiw niffagryii
      481 llmglfsiyt giiyndlfsk sfnifgssws irynkstime nnvlqlnpgt dayaqvpypm
      541 gldpvwmlae nkiiflnsyk mklsiifgvv hmifgvcvsi vnilnfkkys slfleflpql
      601 lflillffyl vllmfvkwil ydasstdvvy spgcapsvli tfinmilmgh tvipgcpeym
      661 fpgqqilqqv cvalaalcip vmlfgrpvyy lctknkkkad rvwsngstsq dielqteglq
      721 tdgpsnpeaa gnhdqenesf geimihqaih tieyvlstis htasylrlwa lslahgqlse
      781 vlwtmvlrag lgakdgsyvy sgllfvsfaa wafftlailv mmeglsaflh tlrlhwvefm
      841 skfydgqgya fqpfcfknil dtedlee
//
LOCUS       XP_034185652             867 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185652
VERSION     XP_034185652.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329761.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..867
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X1"
                     /calculated_mol_wt=98836
     Region          27..856
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..867
                     /gene="Vha100-2"
                     /coded_by="XM_034329761.2:41..2644"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff tenqdsylll yqqeeandsi trtlineeaq nssaigrgrl
      181 efvagvinre rvpafermlw risrgnvflr qaeldrpled patsenvmsv tageqrysrh
      241 sgnkmyktaf vaffqgeqlk srikkvcsgf haslypcphs haervdmvkg vrtrledlnl
      301 vlnqthdhrq rvlhnvakel pnwmimvrkm kaiyhtmnlf nmdvskkcli gecwvpvadl
      361 givrnclteg srlcgssips flnviytden pptfnrtnkf trgfqnlida ygvasyrean
      421 palytiitfp flfsimfgdc ghglimtlfa lymiilekkl maqkstseiw niffagryii
      481 llmglfsiyt giiyndlfsk sfnifgssws irynkstime nnvlqlnpgt dayaqvpypm
      541 gldpvwmlae nkiiflnsyk mklsiifgvv hmifgvcvsi vnilnfkkys slfleflpql
      601 lflillffyl vllmfvkwil ydasstdvvy spgcapsvli tfinmilmgh tvipgcpeym
      661 fpgqqilqqv cvalaalcip vmlfgrpvyy lctknkkkad rvwsngstsq dielqteglq
      721 tdgpsnpeaa gnhdqenesf geimihqaih tieyvlstis htasylrlwa lslahgqlse
      781 vlwtmvlrag lgakdgsyvy sgllfvsfaa wafftlailv mmeglsaflh tlrlhwvefm
      841 skfydgqgya fqpfcfknil dtedlee
//
LOCUS       XP_034185654             862 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185654
VERSION     XP_034185654.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329763.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..862
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X2"
                     /calculated_mol_wt=98275
     Region          27..851
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..862
                     /gene="Vha100-2"
                     /coded_by="XM_034329763.2:192..2780"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff tenqdsylll yqqeeandsi trtlineeaq nssaigrgrl
      181 efvagvinre rvpafermlw risrgnvflr qaeldrpled patsvtageq rysrhsgnkm
      241 yktafvaffq geqlksrikk vcsgfhasly pcphshaerv dmvkgvrtrl edlnlvlnqt
      301 hdhrqrvlhn vakelpnwmi mvrkmkaiyh tmnlfnmdvs kkcligecwv pvadlgivrn
      361 cltegsrlcg ssipsflnvi ytdenpptfn rtnkftrgfq nlidaygvas yreanpalyt
      421 iitfpflfsi mfgdcghgli mtlfalymii lekklmaqks tseiwniffa gryiillmgl
      481 fsiytgiiyn dlfsksfnif gsswsirynk stimennvlq lnpgtdayaq vpypmgldpv
      541 wmlaenkiif lnsykmklsi ifgvvhmifg vcvsivniln fkkysslfle flpqllflil
      601 lffylvllmf vkwilydass tdvvyspgca psvlitfinm ilmghtvipg cpeymfpgqq
      661 ilqqvcvala alcipvmlfg rpvyylctkn kkkadrvwsn gstsqdielq teglqtdgps
      721 npeaagnhdq enesfgeimi hqaihtieyv lstishtasy lrlwalslah gqlsevlwtm
      781 vlraglgakd gsyvysgllf vsfaawafft lailvmmegl saflhtlrlh wvefmskfyd
      841 gqgyafqpfc fknildtedl ee
//
LOCUS       XP_034185655             856 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185655
VERSION     XP_034185655.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329764.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..856
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X3"
                     /calculated_mol_wt=97469
     Region          27..845
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..856
                     /gene="Vha100-2"
                     /coded_by="XM_034329764.2:192..2762"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff teeeandsit rtlineeaqn ssaigrgrle fvagvinrer
      181 vpafermlwr isrgnvflrq aeldrpledp atsenvmsvt ageqrysrhs gnkmyktafv
      241 affqgeqlks rikkvcsgfh aslypcphsh aervdmvkgv rtrledlnlv lnqthdhrqr
      301 vlhnvakelp nwmimvrkmk aiyhtmnlfn mdvskkclig ecwvpvadlg ivrncltegs
      361 rlcgssipsf lnviytdenp ptfnrtnkft rgfqnliday gvasyreanp alytiitfpf
      421 lfsimfgdcg hglimtlfal ymiilekklm aqkstseiwn iffagryiil lmglfsiytg
      481 iiyndlfsks fnifgsswsi rynkstimen nvlqlnpgtd ayaqvpypmg ldpvwmlaen
      541 kiiflnsykm klsiifgvvh mifgvcvsiv nilnfkkyss lfleflpqll flillffylv
      601 llmfvkwily dasstdvvys pgcapsvlit finmilmght vipgcpeymf pgqqilqqvc
      661 valaalcipv mlfgrpvyyl ctknkkkadr vwsngstsqd ielqteglqt dgpsnpeaag
      721 nhdqenesfg eimihqaiht ieyvlstish tasylrlwal slahgqlsev lwtmvlragl
      781 gakdgsyvys gllfvsfaaw afftlailvm meglsaflht lrlhwvefms kfydgqgyaf
      841 qpfcfknild tedlee
//
LOCUS       XP_034185656             854 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185656
VERSION     XP_034185656.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329765.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..854
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X4"
                     /calculated_mol_wt=97376
     Region          27..843
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..854
                     /gene="Vha100-2"
                     /coded_by="XM_034329765.2:192..2756"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff tenqdsylll yqqeeandsi trtlineeaq nssaigrgrl
      181 efvagvinre rvpafermlw risrgnvflr qaeldrpled patsenvmgn kmyktafvaf
      241 fqgeqlksri kkvcsgfhas lypcphshae rvdmvkgvrt rledlnlvln qthdhrqrvl
      301 hnvakelpnw mimvrkmkai yhtmnlfnmd vskkcligec wvpvadlgiv rncltegsrl
      361 cgssipsfln viytdenppt fnrtnkftrg fqnlidaygv asyreanpal ytiitfpflf
      421 simfgdcghg limtlfalym iilekklmaq kstseiwnif fagryiillm glfsiytgii
      481 yndlfsksfn ifgsswsiry nkstimennv lqlnpgtday aqvpypmgld pvwmlaenki
      541 iflnsykmkl siifgvvhmi fgvcvsivni lnfkkysslf leflpqllfl illffylvll
      601 mfvkwilyda sstdvvyspg capsvlitfi nmilmghtvi pgcpeymfpg qqilqqvcva
      661 laalcipvml fgrpvyylct knkkkadrvw sngstsqdie lqteglqtdg psnpeaagnh
      721 dqenesfgei mihqaihtie yvlstishta sylrlwalsl ahgqlsevlw tmvlraglga
      781 kdgsyvysgl lfvsfaawaf ftlailvmme glsaflhtlr lhwvefmskf ydgqgyafqp
      841 fcfknildte dlee
//
LOCUS       XP_034185657             849 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185657
VERSION     XP_034185657.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329766.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..849
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X5"
                     /calculated_mol_wt=96816
     Region          27..838
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..849
                     /gene="Vha100-2"
                     /coded_by="XM_034329766.2:192..2741"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff tenqdsylll yqqeeandsi trtlineeaq nssaigrgrl
      181 efvagvinre rvpafermlw risrgnvflr qaeldrpled patgnkmykt afvaffqgeq
      241 lksrikkvcs gfhaslypcp hshaervdmv kgvrtrledl nlvlnqthdh rqrvlhnvak
      301 elpnwmimvr kmkaiyhtmn lfnmdvskkc ligecwvpva dlgivrnclt egsrlcgssi
      361 psflnviytd enpptfnrtn kftrgfqnli daygvasyre anpalytiit fpflfsimfg
      421 dcghglimtl falymiilek klmaqkstse iwniffagry iillmglfsi ytgiiyndlf
      481 sksfnifgss wsirynksti mennvlqlnp gtdayaqvpy pmgldpvwml aenkiiflns
      541 ykmklsiifg vvhmifgvcv sivnilnfkk ysslfleflp qllflillff ylvllmfvkw
      601 ilydasstdv vyspgcapsv litfinmilm ghtvipgcpe ymfpgqqilq qvcvalaalc
      661 ipvmlfgrpv yylctknkkk adrvwsngst sqdielqteg lqtdgpsnpe aagnhdqene
      721 sfgeimihqa ihtieyvlst ishtasylrl walslahgql sevlwtmvlr aglgakdgsy
      781 vysgllfvsf aawafftlai lvmmeglsaf lhtlrlhwve fmskfydgqg yafqpfcfkn
      841 ildtedlee
//
LOCUS       XP_034185658             838 aa            linear   INV 03-SEP-2025
DEFINITION  V-type ATPase subunit a family protein Vha100-2 isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185658
VERSION     XP_034185658.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329767.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="V-type ATPase subunit a family protein Vha100-2
                     isoform X6"
                     /calculated_mol_wt=95449
     Region          27..827
                     /region_name="V_ATPase_I"
                     /note="V-type ATPase 116kDa subunit family; pfam01496"
                     /db_xref="CDD:460232"
     CDS             1..838
                     /gene="Vha100-2"
                     /coded_by="XM_034329767.2:192..2708"
                     /db_xref="GeneID:117606819"
ORIGIN      
        1 mgalfrseem alcqlfiqpe sayssvselg etgtvqfrdl nadvnyfqrk fvnevrrcde
       61 merklryiea evrkdgvpia dnltelprap npraiidlea hlektendil elsqnavnlk
      121 snyleltelr hvlektqvff teeeandsit rtlineeaqn ssaigrgrle fvagvinrer
      181 vpafermlwr isrgnvflrq aeldrpledp atgnkmykta fvaffqgeql ksrikkvcsg
      241 fhaslypcph shaervdmvk gvrtrledln lvlnqthdhr qrvlhnvake lpnwmimvrk
      301 mkaiyhtmnl fnmdvskkcl igecwvpvad lgivrnclte gsrlcgssip sflnviytde
      361 npptfnrtnk ftrgfqnlid aygvasyrea npalytiitf pflfsimfgd cghglimtlf
      421 alymiilekk lmaqkstsei wniffagryi illmglfsiy tgiiyndlfs ksfnifgssw
      481 sirynkstim ennvlqlnpg tdayaqvpyp mgldpvwmla enkiiflnsy kmklsiifgv
      541 vhmifgvcvs ivnilnfkky sslfleflpq llflillffy lvllmfvkwi lydasstdvv
      601 yspgcapsvl itfinmilmg htvipgcpey mfpgqqilqq vcvalaalci pvmlfgrpvy
      661 ylctknkkka drvwsngsts qdielqtegl qtdgpsnpea agnhdqenes fgeimihqai
      721 htieyvlsti shtasylrlw alslahgqls evlwtmvlra glgakdgsyv ysgllfvsfa
      781 awafftlail vmmeglsafl htlrlhwvef mskfydgqgy afqpfcfkni ldtedlee
//
LOCUS       XP_034185427             597 aa            linear   INV 03-SEP-2025
DEFINITION  rab escort protein [Osmia lignaria lignaria].
ACCESSION   XP_034185427
VERSION     XP_034185427.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329536.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="rab escort protein"
                     /calculated_mol_wt=66883
     Region          1..454
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..597
                     /gene="Rep"
                     /coded_by="XM_034329536.2:147..1940"
                     /db_xref="GeneID:117606722"
ORIGIN      
        1 medylpneyd vvvvgtgmte sivaaaasri gkkvlhldsn eyygglwatf nfdglqkwid
       61 dlkvsgnntk nafnldlnpe eqfleasnqy stvenieetw yiakeadlpv vsskdtqtdg
      121 ngdnsgsgde kvdddkiekk envkqwsier ikkeyrkfni dlapkllfar gelvelliss
      181 niaryaefra vsrvatfmdg kltqvpcsra dvfanktvsv vekrmlmqll tscmeqgads
      241 pefdgfrdkt fleylntksl tpivqhyvvq aiamatdkts crdgvnrtkh flnslgrygn
      301 tpflwpmygs gelpqcfcrl cavfggvycl krqldgviih kdkckaiisg kqrislehlv
      361 vgqghlppei vasegeqris rgifitdrsi mqgekenltl lyyppeepdq epvtvielgp
      421 stnacpqglf mvhmtckrtt nakedlsyav nkflkaepld psatrssihs htqtvspdkr
      481 hiqegeennk eepvestkpq vlwslylnlp asdiklkdsa pinlhlcsgp dldldfdfav
      541 nqakcifkam ypeedflpra pdpeeivleg eeapgpkfeg dteaeekqdi ekaeked
//
LOCUS       XP_034185429             597 aa            linear   INV 03-SEP-2025
DEFINITION  rab escort protein [Osmia lignaria lignaria].
ACCESSION   XP_034185429
VERSION     XP_034185429.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329538.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="rab escort protein"
                     /calculated_mol_wt=66883
     Region          1..454
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..597
                     /gene="Rep"
                     /coded_by="XM_034329538.2:166..1959"
                     /db_xref="GeneID:117606722"
ORIGIN      
        1 medylpneyd vvvvgtgmte sivaaaasri gkkvlhldsn eyygglwatf nfdglqkwid
       61 dlkvsgnntk nafnldlnpe eqfleasnqy stvenieetw yiakeadlpv vsskdtqtdg
      121 ngdnsgsgde kvdddkiekk envkqwsier ikkeyrkfni dlapkllfar gelvelliss
      181 niaryaefra vsrvatfmdg kltqvpcsra dvfanktvsv vekrmlmqll tscmeqgads
      241 pefdgfrdkt fleylntksl tpivqhyvvq aiamatdkts crdgvnrtkh flnslgrygn
      301 tpflwpmygs gelpqcfcrl cavfggvycl krqldgviih kdkckaiisg kqrislehlv
      361 vgqghlppei vasegeqris rgifitdrsi mqgekenltl lyyppeepdq epvtvielgp
      421 stnacpqglf mvhmtckrtt nakedlsyav nkflkaepld psatrssihs htqtvspdkr
      481 hiqegeennk eepvestkpq vlwslylnlp asdiklkdsa pinlhlcsgp dldldfdfav
      541 nqakcifkam ypeedflpra pdpeeivleg eeapgpkfeg dteaeekqdi ekaeked
//
LOCUS       XP_034185430             597 aa            linear   INV 03-SEP-2025
DEFINITION  rab escort protein [Osmia lignaria lignaria].
ACCESSION   XP_034185430
VERSION     XP_034185430.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329539.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="rab escort protein"
                     /calculated_mol_wt=66883
     Region          1..454
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     CDS             1..597
                     /gene="Rep"
                     /coded_by="XM_034329539.2:174..1967"
                     /db_xref="GeneID:117606722"
ORIGIN      
        1 medylpneyd vvvvgtgmte sivaaaasri gkkvlhldsn eyygglwatf nfdglqkwid
       61 dlkvsgnntk nafnldlnpe eqfleasnqy stvenieetw yiakeadlpv vsskdtqtdg
      121 ngdnsgsgde kvdddkiekk envkqwsier ikkeyrkfni dlapkllfar gelvelliss
      181 niaryaefra vsrvatfmdg kltqvpcsra dvfanktvsv vekrmlmqll tscmeqgads
      241 pefdgfrdkt fleylntksl tpivqhyvvq aiamatdkts crdgvnrtkh flnslgrygn
      301 tpflwpmygs gelpqcfcrl cavfggvycl krqldgviih kdkckaiisg kqrislehlv
      361 vgqghlppei vasegeqris rgifitdrsi mqgekenltl lyyppeepdq epvtvielgp
      421 stnacpqglf mvhmtckrtt nakedlsyav nkflkaepld psatrssihs htqtvspdkr
      481 hiqegeennk eepvestkpq vlwslylnlp asdiklkdsa pinlhlcsgp dldldfdfav
      541 nqakcifkam ypeedflpra pdpeeivleg eeapgpkfeg dteaeekqdi ekaeked
//
LOCUS       XP_076545667             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_076545667
VERSION     XP_076545667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689552.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_076689552.1:1177..2862"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_034185659             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_034185659
VERSION     XP_034185659.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329768.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_034329768.2:955..2640"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_034185660             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_034185660
VERSION     XP_034185660.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_034329769.2:1023..2708"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_034185661             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_034185661
VERSION     XP_034185661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_034329770.2:972..2657"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_034185662             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_034185662
VERSION     XP_034185662.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329771.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_034329771.2:1377..3062"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_076545668             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_076545668
VERSION     XP_076545668.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689553.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_076689553.1:772..2457"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_034185665             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_034185665
VERSION     XP_034185665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329774.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_034329774.2:474..2159"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_034185666             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_034185666
VERSION     XP_034185666.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329775.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_034329775.2:428..2113"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_034185667             561 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter protein [Osmia lignaria lignaria].
ACCESSION   XP_034185667
VERSION     XP_034185667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="organic cation transporter protein"
                     /calculated_mol_wt=62116
     Region          16..505
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..561
                     /gene="LOC117606821"
                     /coded_by="XM_034329776.2:566..2251"
                     /db_xref="GeneID:117606821"
ORIGIN      
        1 masvdrnlee lmshlgefgk yqcwqfslhi igaltaglhm ltlltvaavp pyecnipest
       61 fpnitdpntn vsltelprai dschyldten vikdceswty dtryfqssrg mewnfvcsqr
      121 wmgavaqsty mfgvffgavt lgsladkhgr kiifyvssva qlvlgvsval innyyfflvi
      181 rflygifgsa gayitgfvlt melvgaskrt vcgvmfqlaf atgfmlvavw gavikdrmwl
      241 qivyglhsal lighwwlmde sprwlwaqgr isealviirk glrmngnnve idtekliseg
      301 kmrnteqedr sygaidlfkt pnlrkktlnv clnwfansiv yyglslnsgn lvgnpflmlf
      361 lnglvelpay iliclvmdrt grrclvstfm liggvccila stiptgtdis saaivtmvlv
      421 gkaciavsfa viynytaelf ptvvrntalg igsmcarlsg sltplimlld sfnpkvpavl
      481 fgfvalvsgf lslylpetvn qampesiedg enfgkhdtcf ttcmgtrrks sssyevtmdq
      541 lgdkdekekl nqtesakesp r
//
LOCUS       XP_076545669             497 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606724 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545669
VERSION     XP_076545669.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689554.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="uncharacterized protein LOC117606724 isoform X1"
                     /calculated_mol_wt=54484
     Region          124..144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(129,131,133,135..136,139..140,143,157,159,163..164,
                     167..168,171,185,187,189,191..192,195..196)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          136..159
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          152..172
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          180..196
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          446..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(453..455,457..458,462,472,479,482..486,488..489)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          474..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..497
                     /gene="LOC117606724"
                     /coded_by="XM_076689554.1:542..2035"
                     /db_xref="GeneID:117606724"
ORIGIN      
        1 msdgvdaggg enevdshscs qtdvgessnq reeenldpdn eaalntnyds sdgavpafrs
       61 vifmcercen yetinktafc ahqdqclasa epgesaenie aaendgdgee shpgirshrk
      121 mfecdvcnmq fsnganmrrh kmrhtgvkpy ecrvcqkrff rkdhlaehft thsktlpyhc
      181 picnrgfqrq iamrahfqne hvgqhdmvks cplcnyraat mkclrlhffn rhgidldnpg
      241 pnsssslmns ctpgeampsa plsdsgdstg nrsadnatpp mhfltphiei sipypeqtpn
      301 qrnpspapln gdspnspqsa dsnsnapsss hhqlpinssg ihrslggned titpsislip
      361 ikqepnsngt eengatssnl plpslikvsp lksllrddlr rklssgsgnn nnnnnngnnt
      421 snsnshsrge ppsstrpdqr snglqcahck itfpdqtlyf lhkgchsesn pwkcnicgeq
      481 ccnlyqfnsh llskshq
//
LOCUS       XP_076545670             497 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606724 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545670
VERSION     XP_076545670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689555.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="uncharacterized protein LOC117606724 isoform X1"
                     /calculated_mol_wt=54484
     Region          124..144
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(129,131,133,135..136,139..140,143,157,159,163..164,
                     167..168,171,185,187,189,191..192,195..196)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          136..159
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          152..172
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          180..196
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          446..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(453..455,457..458,462,472,479,482..486,488..489)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          474..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..497
                     /gene="LOC117606724"
                     /coded_by="XM_076689555.1:657..2150"
                     /db_xref="GeneID:117606724"
ORIGIN      
        1 msdgvdaggg enevdshscs qtdvgessnq reeenldpdn eaalntnyds sdgavpafrs
       61 vifmcercen yetinktafc ahqdqclasa epgesaenie aaendgdgee shpgirshrk
      121 mfecdvcnmq fsnganmrrh kmrhtgvkpy ecrvcqkrff rkdhlaehft thsktlpyhc
      181 picnrgfqrq iamrahfqne hvgqhdmvks cplcnyraat mkclrlhffn rhgidldnpg
      241 pnsssslmns ctpgeampsa plsdsgdstg nrsadnatpp mhfltphiei sipypeqtpn
      301 qrnpspapln gdspnspqsa dsnsnapsss hhqlpinssg ihrslggned titpsislip
      361 ikqepnsngt eengatssnl plpslikvsp lksllrddlr rklssgsgnn nnnnnngnnt
      421 snsnshsrge ppsstrpdqr snglqcahck itfpdqtlyf lhkgchsesn pwkcnicgeq
      481 ccnlyqfnsh llskshq
//
LOCUS       XP_034185440             492 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606724 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185440
VERSION     XP_034185440.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329549.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..492
                     /product="uncharacterized protein LOC117606724 isoform X2"
                     /calculated_mol_wt=53906
     Region          119..139
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(124,126,128,130..131,134..135,138,152,154,158..159,
                     162..163,166,180,182,184,186..187,190..191)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          131..154
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          147..167
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          175..191
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          441..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(448..450,452..453,457,467,474,477..481,483..484)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          469..491
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..492
                     /gene="LOC117606724"
                     /coded_by="XM_034329549.2:541..2019"
                     /db_xref="GeneID:117606724"
ORIGIN      
        1 msdgvdaggg enevdshscs qtdvgessnq reeenldpdn eaalntnyds sdgavpafrc
       61 ercenyetin ktafcahqdq clasaepges aenieaaend gdgeeshpgi rshrkmfecd
      121 vcnmqfsnga nmrrhkmrht gvkpyecrvc qkrffrkdhl aehftthskt lpyhcpicnr
      181 gfqrqiamra hfqnehvgqh dmvkscplcn yraatmkclr lhffnrhgid ldnpgpnsss
      241 slmnsctpge ampsaplsds gdstgnrsad natppmhflt phieisipyp eqtpnqrnps
      301 paplngdspn spqsadsnsn apssshhqlp inssgihrsl ggnedtitps islipikqep
      361 nsngteenga tssnlplpsl ikvsplksll rddlrrklss gsgnnnnnnn ngnntsnsns
      421 hsrgeppsst rpdqrsnglq cahckitfpd qtlyflhkgc hsesnpwkcn icgeqccnly
      481 qfnshllsks hq
//
LOCUS       XP_034185441             406 aa            linear   INV 03-SEP-2025
DEFINITION  lipase 3 [Osmia lignaria lignaria].
ACCESSION   XP_034185441
VERSION     XP_034185441.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329550.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185441.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="lipase 3"
                     /calculated_mol_wt=46379
     Region          16..404
                     /region_name="PLN02872"
                     /note="triacylglycerol lipase"
                     /db_xref="CDD:215470"
     CDS             1..406
                     /gene="LOC117606725"
                     /coded_by="XM_034329550.2:104..1324"
                     /db_xref="GeneID:117606725"
ORIGIN      
        1 mtsvanqdep hmttpeiiat hdyipethni wtedgyclnv hrvlpknthe hshdinknet
       61 ilndnstkne nydihnvnkr vpilihhgll sssadwvllg pgkalayllc dngydvwlgn
      121 argnaysrkh keyttrdref wnfswheigy ydlpttidyi leytgytely yvgysqgtta
      181 fyimasekpe ynrkikgmvs lapvaflsnq rspllklivh fyglmewgss ycnvhqwfpr
      241 srlqaralgt lirnapdslt kgfcvcwfsl iagfgsdqld ksmlplilgh fpagasakqi
      301 ihysqsilsg sfrkfdygaq enlkiygstq ppiydlekik vpiaifysdn dfltdpadvk
      361 kltdrlpnvi gtwkiscpkf nhvdylwgkd vksliynpli avlkkf
//
LOCUS       XP_034185442             330 aa            linear   INV 03-SEP-2025
DEFINITION  dolichyl-diphosphooligosaccharide--protein glycosyltransferase
            subunit TUSC3-like [Osmia lignaria lignaria].
ACCESSION   XP_034185442
VERSION     XP_034185442.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329551.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase subunit TUSC3-like"
                     /calculated_mol_wt=37400
     Region          38..325
                     /region_name="OST3_OST6"
                     /note="OST3 / OST6 family, transporter family; pfam04756"
                     /db_xref="CDD:461420"
     CDS             1..330
                     /gene="LOC117606726"
                     /coded_by="XM_034329551.2:141..1133"
                     /db_xref="GeneID:117606726"
ORIGIN      
        1 mdcknfcifl glllslnyvc cqyrvkntqg sslsdrvqql telaltrpvi kfngakfkey
       61 vktmprnysv ivmftamapq rqcqicrhan defvivansf rylqahskkl ffasvdfdeg
      121 sdvfqmmrln tapvymhfpp kgkpkpadtm diqrvgfgae aiakwisert diqirvfrpp
      181 nysgtvaivm lliliggfly lrrnnldfiy nktvwglgal fftltmisgq mwnhirgppf
      241 ihkspsgnva yihassqgqf iletyivmvl ygavvlgmil mieaasrkdd vkkrrifaii
      301 gaglvaiffs lllsifrnka qgypysllfk
//
LOCUS       XP_034192338             107 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609779 [Osmia lignaria lignaria].
ACCESSION   XP_034192338
VERSION     XP_034192338.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336447.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..107
                     /product="uncharacterized protein LOC117609779"
                     /calculated_mol_wt=12291
     CDS             1..107
                     /gene="LOC117609779"
                     /coded_by="XM_034336447.1:87..410"
                     /db_xref="GeneID:117609779"
ORIGIN      
        1 mdegyihlkv nhsinfkdpe tgthtnaies swrhakgtmg sgtrkkamfp phlakymfmk
       61 mcryqkmdpc etfyrmagel ysplkskvem sdeeqsteid etdenfa
//
LOCUS       XP_076545671             213 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305526 [Osmia lignaria lignaria].
ACCESSION   XP_076545671
VERSION     XP_076545671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689556.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 19% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..213
                     /product="uncharacterized protein LOC143305526"
                     /calculated_mol_wt=24219
     CDS             1..213
                     /gene="LOC143305526"
                     /coded_by="XM_076689556.1:328..969"
                     /db_xref="GeneID:143305526"
ORIGIN      
        1 maqiqnlyld ilnercklek rtiynslsia sispdefayn lmehivcple ksallntiar
       61 gmlgeesvqg glinnlften tlshiaentw nkfvekfikf gsissavimi liiihickli
      121 idtiirgytl htlygwsikl lgaifssity llvtlknnkn ikklpkkrnl arnedielne
      181 ikierpntln wrtlppvllr ppaikppfis ynv
//
LOCUS       XP_076545672             722 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117611452 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545672
VERSION     XP_076545672.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689557.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 25% of CDS bases
            frameshifts          :: corrected 3 indels
            internal stop codons :: corrected 1 genomic stop codons
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..722
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117611452"
                     /calculated_mol_wt=84242
     Region          319..390
                     /region_name="HTH_Tnp_Tc5"
                     /note="Tc5 transposase DNA-binding domain; cl12115"
                     /db_xref="CDD:472278"
     Region          652..713
                     /region_name="Transp_Tc5_C"
                     /note="Tc5 transposase C-terminal domain; pfam04236"
                     /db_xref="CDD:398081"
     CDS             1..722
                     /gene="LOC117611452"
                     /coded_by="XM_076689557.1:1..2169"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 3 bases in 3 codons;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:117611452"
ORIGIN      
        1 midldtsggk esgegegker vdelkeskes rreireaida tktgkpeqgp riidhrnkrn
       61 rrqksvnklc eisaqalsqy qesvkkvetv tvnvndmice vrkeltswre ermtenqrtr
      121 dlrqtvrenh aiqiqrvydm vgpqrcrsgp crtapavtca npssswcgpg tgnskedlty
      181 niayfkdilm kfeliyaepp kkftrfvkev agklfenfvk mlrextiaee veyfssneee
      241 eqkltpptle eeyvpsckni ssldrvpfdi klkivmtvnk hptwsfyglq trfkrhlrhk
      301 tdtarfrkdi ikggtytdkm eiikknvydr ftetrrreql vtrrqlqqwa mdaamqfrda
      361 vnedgsnfqf ttsaswitnf lkvyrisnrr vvryvsnkei tspeeimksa iqfqnliqsi
      421 sndydsdyii ntdqtsceyr invartytht gqktvelfig dlnkithsya aqysltksgk
      481 llpkvfvclq esgdafgvrv kaevnellkl cknvivvcsk sgklssdlyk kylekvikpy
      541 vgnnpflmiv dswgghknih myneifvnse nrptcnlqii ppkctpicqp cdvyfyrqvk
      601 ilikriqnys qdimadrekk elntrydair iqslihylls aplfkkmiqf awyasklene
      661 rdifynvnql cfppdihtkk cicnfrfsfi tyswctnylc fncfyvkfhp qhctamtefy
      721 nk
//
LOCUS       XP_034194870             368 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611082 [Osmia lignaria lignaria].
ACCESSION   XP_034194870
VERSION     XP_034194870.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338979.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194870.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 35% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..368
                     /product="uncharacterized protein LOC117611082"
                     /calculated_mol_wt=42191
     CDS             1..368
                     /gene="LOC117611082"
                     /coded_by="XM_034338979.2:95..1201"
                     /db_xref="GeneID:117611082"
ORIGIN      
        1 mrmsldgaqp ikpaflptan trnantngir khfeqtsdyr revqlprmns nmemehrpkr
       61 mankparyqt tssdeeprkr lkvapgsive ditdlratle gvddevlsnn yrqhehtnty
      121 tpldtyspyt stytpsnini ysphtppnty rppptayapp tqsfhipdav esrpeprmqq
      181 gteptnlqir dlytkignle smvtkthsll ekvakqqqhl lhqhhgipsk paflpissla
      241 rmtefqnide dsyrevvnyl eyvggfnlke avtlsfketv sdclltsysw lgrednnlal
      301 cetrivhaih davlqnkhfp rpsrkdvaeh lkaalrtake rvrskrrgpr npqhetvdnf
      361 wtakeqsr
//
LOCUS       XP_076545673             283 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305527 [Osmia lignaria lignaria].
ACCESSION   XP_076545673
VERSION     XP_076545673.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689558.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 79% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="uncharacterized protein LOC143305527"
                     /calculated_mol_wt=31376
     CDS             1..283
                     /gene="LOC143305527"
                     /coded_by="XM_076689558.1:1..852"
                     /db_xref="GeneID:143305527"
ORIGIN      
        1 mavarsptrl drdamtagep eeaflpsvsa gtnatvysas lkklppfwke npvlwfaqve
       61 aafdlarits detkyrhvit hldqvvlpfv adiltqppar gkyeaiknki igtfdespes
      121 klrhvlrgrg lgdekpsyyl qrirnlaggq isenvlrslf ldqlpdnirs ilaisetqdl
      181 gklalqadki iemqainvaa vssapravsp hgrmaalqdq rrrrsgsreg rsphrarssd
      241 glcfyhrrfa erahrcvipc swtgpppqgl pisrpslqlq pen
//
LOCUS       XP_076545674             361 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305528 [Osmia lignaria lignaria].
ACCESSION   XP_076545674
VERSION     XP_076545674.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689559.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..361
                     /product="uncharacterized protein LOC143305528"
                     /calculated_mol_wt=40872
     CDS             1..361
                     /gene="LOC143305528"
                     /coded_by="XM_076689559.1:1..1086"
                     /db_xref="GeneID:143305528"
ORIGIN      
        1 mrdiatsgti ckfsdehcid megghtfwsa lpredclktk ydvlyrglvt kltsnnreil
       61 ytiettdisf alaakgdikt cnriltkteh prliiykrtq nnilfdnspd efaynlmekp
      121 gyiariagev vhiikctpke vqllhtdkcy tqlpvtsgnq trfltpkthi lmksgtqiec
      181 ntiipqyykl nnnwialtpt prkaqkpgil kpntniswey keinslassg iysqedlqel
      241 qqyimfalek sallntiarg mlgeesvqgg linnlftent lshiaentwn kfvekfikfg
      301 sissavimil iiihicklii dtiireytlh tvygwfilll gaifssityl lgktskgktp
      361 n
//
LOCUS       XP_076545675             325 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305529 [Osmia lignaria lignaria].
ACCESSION   XP_076545675
VERSION     XP_076545675.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689560.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..325
                     /product="uncharacterized protein LOC143305529"
                     /calculated_mol_wt=37067
     CDS             1..325
                     /gene="LOC143305529"
                     /coded_by="XM_076689560.1:1..978"
                     /db_xref="GeneID:143305529"
ORIGIN      
        1 mllesakqnp yphknltptv hkilchgeyi igfhilpige lseeaqeanh keyrrlrlnd
       61 plisslrmqq krkdiqeewi ptrssnness iweyrdemvt knidsisnde ealnlelrht
      121 gdkvwkftfn kdknkhivfi atykqenaqf epknedksmi iilkqagllt qetfsqlikl
      181 acqngyklmt plagacfnkd dtsslatelv ipevdlliki srstqsgghy lkysdvdiav
      241 casiaatrnv kdeilkksiv vkilkqyink gfipnkarmt iiskyatggi pskfiyeeiv
      301 kvfdseqtky salkivqttk qsepl
//
LOCUS       XP_076545676             129 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305532 [Osmia lignaria lignaria].
ACCESSION   XP_076545676
VERSION     XP_076545676.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689561.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..129
                     /product="uncharacterized protein LOC143305532"
                     /calculated_mol_wt=15054
     CDS             1..129
                     /gene="LOC143305532"
                     /coded_by="XM_076689561.1:1..390"
                     /db_xref="GeneID:143305532"
ORIGIN      
        1 mnyyadirie kfksghhpen gvrleddirp ercpcflcve aedevylkkk lpentprdgk
       61 sidctgiwpr ntgsmyidts rsirvdhqpe eggqgvkiwe kmeeeeekip dnkqqqpkel
      121 yillqhlvr
//
LOCUS       XP_076545677             193 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305239, partial
            [Osmia lignaria lignaria].
ACCESSION   XP_076545677
VERSION     XP_076545677.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689562.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..193
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305239"
     Region          114..146
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..193
                     /gene="LOC143305239"
                     /coded_by="XM_076689562.1:<1..582"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     deleted 1 base in 1 codon"
                     /db_xref="GeneID:143305239"
ORIGIN      
        1 acrkslkkkk dtksgqatnk ikkwihedem sfltkymker etvttiqina seesnneadd
       61 ttiednsnli ktqappqvsh tltefrekkr kknrtgsevl mnyilntnee ktmdeidlff
      121 ksaaltvkkl tpynqiitki rissiiselq lxelsninch hfssqslnst ptpsssttep
      181 lqptasnfyy tsi
//
LOCUS       XP_076545678             229 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305533 [Osmia lignaria lignaria].
ACCESSION   XP_076545678
VERSION     XP_076545678.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689563.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..229
                     /product="uncharacterized protein LOC143305533"
                     /calculated_mol_wt=27383
     Region          34..150
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..229
                     /gene="LOC143305533"
                     /coded_by="XM_076689563.1:2486..3175"
                     /db_xref="GeneID:143305533"
ORIGIN      
        1 mllyyrypst fekweriqrg fehrwnfpnv igaldgkhvn iilpissgsd yynykgkysi
       61 vlmalvdddy rftytyrcws shayrpwqke svplsisekv fnyrlsrvrr iienafgilt
      121 arfrifendi klqpekveki ifsacsqlhn wlrrckneny wlreivnred tefgqiisgh
      181 wrqitrglts vhadrfahnp arhainirne yceyfngvga vpwqlkmin
//
LOCUS       XP_076545679             139 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611090 [Osmia lignaria lignaria].
ACCESSION   XP_076545679
VERSION     XP_076545679.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689564.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..139
                     /product="uncharacterized protein LOC117611090"
                     /calculated_mol_wt=15969
     CDS             1..139
                     /gene="LOC117611090"
                     /coded_by="XM_076689564.1:404..823"
                     /db_xref="GeneID:117611090"
ORIGIN      
        1 mtdmnvetne qneimnallq lqqqlqhmqt rqqerdreva vlqqqlqqqq mlhqqqppqq
       61 qqtpqqqpqp qhqqppqhpd eteadvkprr krgtaagvrr rqnrgrwprg grgggmgagm
      121 gggrggaink yyfsyrrln
//
LOCUS       XP_076545680             324 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305535 [Osmia lignaria lignaria].
ACCESSION   XP_076545680
VERSION     XP_076545680.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689565.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..324
                     /product="uncharacterized protein LOC143305535"
                     /calculated_mol_wt=37015
     Region          <111..244
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..324
                     /gene="LOC143305535"
                     /coded_by="XM_076689565.1:55..1029"
                     /db_xref="GeneID:143305535"
ORIGIN      
        1 msptdfewli kligpkikrn dthwrkaisv qerlavtirf letgdsftsl qylskiskql
       61 istivpevce aliegikeni qipntseawl evakvynnrw nfphclgald vdadynflyv
      121 daggqgrisd ggifkncsly kklqnnelnl lqaepldgrh revpyffigd eafsltstlm
      181 kpfsgihrkg sfqrifnyrl scarrvvenv fgiasaifqi lrkplllepe katlivltva
      241 hlhnflrkss tscrlytphe ifdteregqf vpgtyrsias epmysllsln riprrpstda
      301 tqireeladf flnegsilwq dnys
//
LOCUS       XP_076545681             931 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305536, partial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545681
VERSION     XP_076545681.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689566.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 46% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the carboxy end.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..>931
                     /product="uncharacterized protein LOC143305536"
     Region          168..362
                     /region_name="Helitron_like_N"
                     /note="Helitron helicase-like domain at N-terminus;
                     pfam14214"
                     /db_xref="CDD:464105"
     Region          842..>931
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(848..854,930)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     CDS             1..931
                     /gene="LOC143305536"
                     /coded_by="XM_076689566.1:1..>2793"
                     /db_xref="GeneID:143305536"
ORIGIN      
        1 milmlprnle ddqainvniq rnllqkslhl eeyvqkwtvr awlrylmnkp lyklydvrfn
       61 esffatdvpa iemsepvdca qrkqrdveet pierislkqv sdidvliaqq rtmlwseeky
      121 lalvsgqlki ppntlfdkha eelsfpsiyl gearsfkdrv kvtpfmmaks eirrrdcrga
      181 tpdhvlymal kimrirmqgm rhlfrnvagm snmtrkmied kqylertvkc dmsflrtvpn
      241 salywsqrkr dvfamlrqlg rptmfltlsa sevrwphllr llyktkhgre ydadpmkmds
      301 lsraelvndd pvtsvlyfdk lvdmlisilk hkegafgkyr vrdyfkrikf qhrgspyahi
      361 llwlendpce kvneempdtl amveklcsvd haalenpsnq lhkhrftcfk esakkcrfga
      421 pfwpmsetqf lipmchedkh radlkerydq ihfslehaey ktmnvffedn eikdgheylr
      481 ilragidrpi llykrtleqk wtnvynpwia svlnsnmdlq fileeyacaa hvveyvnktn
      541 rgisnlyrel lklrheypek dytdllkevg lqvlnsvems sqeaawyllg lgmsrssrdi
      601 kyiptvwpee rtktkrtrkq leeenvaddd mniwheclie kyenrpeele qltlaqfvay
      661 ytpgtskrre fariiryrgy aikdllnfkr ekvllhypfr neivdildcn kflqlyqdhe
      721 rviferdkef dldldvnevm rrcaemyedy egeertdera qafvenkrve ggdadfpnaa
      781 andddiktih ttklrgiikk renvmseeey yrsmrctnke qrtlilesiy ryinnssepi
      841 qifftgpagc gktfttkllm etynriagkk vsiynayiac altgkaatai ggvtvqsafk
      901 ifkaqygglq pdqltsfrls lrgvaailid e
//
LOCUS       XP_034194567             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610845 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194567
VERSION     XP_034194567.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338676.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117610845 isoform X1"
                     /calculated_mol_wt=12993
     CDS             1..116
                     /gene="LOC117610845"
                     /coded_by="XM_034338676.2:1445..1795"
                     /db_xref="GeneID:117610845"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lpgfynicrn tspwstqdsp
       61 nilrevgkag mgpkkvsgta selqnteedk eeeeeeeeep elssistpwm asipsa
//
LOCUS       XP_034194568             114 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610845 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194568
VERSION     XP_034194568.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338677.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..114
                     /product="uncharacterized protein LOC117610845 isoform X2"
                     /calculated_mol_wt=12807
     CDS             1..114
                     /gene="LOC117610845"
                     /coded_by="XM_034338677.2:1445..1789"
                     /db_xref="GeneID:117610845"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lpgfynicrn tspwstqdsp
       61 nilrevgkag mgpkkgtase lqnteedkee eeeeeeepel ssistpwmas ipsa
//
LOCUS       XP_034194569             110 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610845 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194569
VERSION     XP_034194569.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338678.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..110
                     /product="uncharacterized protein LOC117610845 isoform X3"
                     /calculated_mol_wt=12395
     CDS             1..110
                     /gene="LOC117610845"
                     /coded_by="XM_034338678.2:1445..1777"
                     /db_xref="GeneID:117610845"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lpgfynicrn tspwstqdsp
       61 nilrevgkav sgtaselqnt eedkeeeeee eeepelssis tpwmasipsa
//
LOCUS       XP_034194570             108 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610845 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194570
VERSION     XP_034194570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338679.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..108
                     /product="uncharacterized protein LOC117610845 isoform X4"
                     /calculated_mol_wt=12208
     CDS             1..108
                     /gene="LOC117610845"
                     /coded_by="XM_034338679.2:1446..1772"
                     /db_xref="GeneID:117610845"
ORIGIN      
        1 mgyvhdehie kpiritrllh ssinadnywp cisfdikmni lpgfynicrn tspwstqdsp
       61 nilrevgkag taselqntee dkeeeeeeee epelssistp wmasipsa
//
LOCUS       XP_034194687             474 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610941 [Osmia lignaria lignaria].
ACCESSION   XP_034194687
VERSION     XP_034194687.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338796.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194687.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="uncharacterized protein LOC117610941"
                     /calculated_mol_wt=54655
     Region          39..>311
                     /region_name="Baculo_F"
                     /note="Baculovirus F protein; pfam12259"
                     /db_xref="CDD:371998"
     CDS             1..474
                     /gene="LOC117610941"
                     /coded_by="XM_034338796.2:1329..2753"
                     /db_xref="GeneID:117610941"
ORIGIN      
        1 mlhfrmilll lisgigittg eikftplnda svfiekqgka hlfythwkli vmadcneptl
       61 riseveymln vtkkacsdvc leakeilmle hklskvkeen irlkiflgir nkrgllnfvg
      121 svsknlfgtl deddlklinq nmdklfeqqn vmsnailnqt hiiktllkea nsdtmfklhr
      181 enfvlyhsai dkinnnsrni ymnsqliais vliteiendv rnfmdamtlg rsgivnpsiv
      241 kpkefvqrle alqrsefkry sidlkeenyq ilisissfti sivnhkiayv feiplideqe
      301 weikrlypip ttrdniiyti pviphntale tnsqiilssq reiekfkryn klrickqksa
      361 shpissskkc eseilqlapd ilkicptgty ymseeltyie lesgkfivip rgeveiqtlc
      421 kehstitirk ahivesdesc iltstnsmik igkvdreyty etrikyivks ydgr
//
LOCUS       XP_076545682            1032 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117610952 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545682
VERSION     XP_076545682.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689567.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 7% of CDS bases
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1032
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117610952"
                     /calculated_mol_wt=117970
     Region          919..>1028
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(975,1009,1019,1021)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..1032
                     /gene="LOC117610952"
                     /coded_by="XM_076689567.1:1649..4747"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:117610952"
ORIGIN      
        1 mdinhaavsa tmvtgggynq leellsgvnm scmtkrmyek cqddiidgfe vaaqqemeea
       61 gkeerelaia rgdvlagyni pwipvvadgs wmrrsyrggd ynslsgvgai vgyhtkkvly
      121 invknkfcmi cqiaakkkea arehrcfknw grqqsstgme saaivegfkc slemhglvys
      181 ilvadgdssv ykkildndpy kdymvhirki ectnhllrnf srkindivak grykelravv
      241 kdnalrlrtg ikkaaeyrfk ekirlvdqik nlkedmknvp shvfgehkec qklayfctkx
      301 sdpdrvnyvp rlkqggiyhs ieeamqplfa qaesllyaln nnvvesfnni iakfiggkri
      361 nfgrrgsyqg rvsaavvqfn sketfsklsm amnkepttiv rkmeerrkra temaskykke
      421 aksssfrnpk qsydqdygps atkpdmeetv ysseyerhma ilhkwqtmrr qkqrqsstmt
      481 ieaaavfeek fyvplksfgk tvkedsqtdm sdknplffpn nvndikrsyg rnynsvsgvg
      541 aivgfrtekv lfigirnkyc ticdtaerka kspkhhkcyk nydryassss tesdaiaegf
      601 nwsidmhgli yrtvvadgds niyhtivnnr pyreqkvtvr kvectnhllr nlckklrhva
      661 ektqtkghrd rgfvahrnii kknilnirrt vvltvkarkq ekgpqhikat elqkdilsip
      721 shifgehkec nnrshmcmrd enraeknyvp slklydlyse vekairflsc ysdsllmnvt
      781 nnpvetfnsi ickeiggkri nfgargsyna rvagtvtqyn tqqvltemhk nmcktvptlv
      841 krleerrqkk vaktkefrmt ygkrrkfkpm qgtdkyygpn sqrpdlpsdm llqlrkehfe
      901 kladnaknrt iietntrkqn dsdlwralke emltasnfga vcrmrqttsc atmvknilyp
      961 psidtaamky grdkehiark dvanamktri rtcglfidrd ipylgaspdg lidddgllel
     1021 kcplsaekgl av
//
LOCUS       XP_034190220             638 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608757 [Osmia lignaria lignaria].
ACCESSION   XP_034190220
VERSION     XP_034190220.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334329.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190220.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..638
                     /product="uncharacterized protein LOC117608757"
                     /calculated_mol_wt=69194
     Region          65..279
                     /region_name="MIP"
                     /note="Major intrinsic protein (MIP) superfamily. Members
                     of the MIP superfamily function as membrane channels that
                     selectively transport water, small neutral molecules, and
                     ions out of and between cells. The channel proteins share
                     a common fold: the N-terminal...; cd00333"
                     /db_xref="CDD:238204"
     Site            order(99,117..119,239..240,243,246)
                     /site_type="active"
                     /note="amphipathic channel [active]"
                     /db_xref="CDD:238204"
     Site            order(119..121,243..245)
                     /site_type="other"
                     /note="Asn-Pro-Ala signature motifs"
                     /db_xref="CDD:238204"
     Region          393..610
                     /region_name="MIP"
                     /note="Major intrinsic protein (MIP) superfamily. Members
                     of the MIP superfamily function as membrane channels that
                     selectively transport water, small neutral molecules, and
                     ions out of and between cells. The channel proteins share
                     a common fold: the N-terminal...; cd00333"
                     /db_xref="CDD:238204"
     Site            order(428,446..448,568..569,572,575)
                     /site_type="active"
                     /note="amphipathic channel [active]"
                     /db_xref="CDD:238204"
     Site            order(448..450,572..574)
                     /site_type="other"
                     /note="Asn-Pro-Ala signature motifs"
                     /db_xref="CDD:238204"
     CDS             1..638
                     /gene="LOC117608757"
                     /coded_by="XM_034334329.2:196..2112"
                     /db_xref="GeneID:117608757"
ORIGIN      
        1 meqggisiip tttiadntvg ksksigitlr pscsiqvake klvtqwisnl mkedasgwei
       61 llvslaemig tailvflgca gcigsldskp svlqtsltfg favmvaiqcm ghisgahinp
      121 citiaslilg kksvpmclfy ivsqcvgall gysllkvitp dqliystdad qrsnfcmtmi
      181 hpnltifrgf mgefiatail vlfacglwdc rnvhntdsap lrfglciavl cfvfspytgc
      241 smnpartfgp avwnnywhnh wvywvaplsa avlasvfyrc lfdckvirdd sgqslngvrt
      301 rrfavestle rpravaikvr tivhkqsrra tvhsappphg fsfsilpnrf esvwlvassr
      361 fydhramsss dlrsgfkrlm qgegalkntv lialaetigt smlvfvgcmg cigslgvvps
      421 hlqialtfgl avmlviqsig hishahinpa itvgsvvlgk ktipeglvyl lsqmigavig
      481 ygmlkvvtpa drltskstie admfcvtdlh adltaiqgll legistgilm lvacavwdrr
      541 nehntdsvpi rfgltvtvla ltfgpytgcs mnparslgpa lwnnqwshhw iywfgpiggs
      601 llssfmykti fglkdlaeev tvpetvalns vetqkaep
//
LOCUS       XP_076545683             373 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305537 [Osmia lignaria lignaria].
ACCESSION   XP_076545683
VERSION     XP_076545683.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689568.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="uncharacterized protein LOC143305537"
                     /calculated_mol_wt=40721
     Region          <101..>249
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     CDS             1..373
                     /gene="LOC143305537"
                     /coded_by="XM_076689568.1:1..1122"
                     /db_xref="GeneID:143305537"
ORIGIN      
        1 mhpkacfalf vlglalayai pasriekksv ndepafneel kaeelgddke rakkstfcme
       61 ikdkgaqagq lgpvqtmnlv pqmhggqhmh ggqqmqafsk ptaqqsvqty sipsgqqsvq
      121 tfnipsaqqt vqpassvqtf nvpsaqqtvq passvqtyni psahqtvqpv qaysipsaqq
      181 qvqtmsviht qsqpaapqtv vipqpvqpih tlqiiqpsqp cpqpsvniqy qqpptkevet
      241 iemnnepept papmpqttpq ppakivkphy itktvmvphl devdmscvpm apvykeqmvv
      301 kpmymmsepq kmatvlkvph cphshsssvv qcncrptsms vepmsimsiq rsnmmgghtl
      361 vprqadatee kka
//
LOCUS       XP_076545684            1838 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606505 [Osmia lignaria lignaria].
ACCESSION   XP_076545684
VERSION     XP_076545684.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689569.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1838
                     /product="uncharacterized protein LOC117606505"
                     /calculated_mol_wt=206279
     Region          <80..741
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <866..1006
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..1838
                     /gene="LOC117606505"
                     /coded_by="XM_076689569.1:257..5773"
                     /db_xref="GeneID:117606505"
ORIGIN      
        1 madtedpeet sapqesdyei pgipedskss ttsasksspk mskieslvtt ilqngspsas
       61 vvmpppddlp rdpsiqsqqe etsileetsk sqeelkksqe lplngkktsl dqlskdmfqn
      121 gqkpraarlh rqcqrrfrel igkremknee lekqqgdlkh rlnilecsmp avmvwniwrm
      181 sqgacvpsik qvleksfqgp asgevycpst psrhfdcrvr eveaerkqaq kraeeartlw
      241 aekaaaleek nkkldearka qeqikeniek lkieaqklre aaaakmkddg acesgecgvi
      301 eckkkwldkv pssasikstd iecleelqql aetelcmkrq iadlerreea ymrtlqqade
      361 lwsklqgdaa srmstlqeql dvktaanqqm adrivqledi leklrtrman crnelekyms
      421 igkiealigr dddfadvveq ktssevlrsd qkvgskysva tveekgvlsk aevsekdala
      481 kpdfadltts vqyddltvee kqkleaakny lasygslsel ftdddyvcap dficndqvft
      541 etglteeelk alkegrvtae mllkkfeead eisevtvggi ssamsktikh tedskeavel
      601 capdficndq vftesgltee emkalkegrm taemllkkle eadeisevtv ggvpsamrkt
      661 ikhtedrkea velvyevgee elisvdeiti ksdrkiddek etpvttleet eidavqeafi
      721 eapmpeeeiv egmtteaeve dytlyqdavg ddnilvprde lqtwqgnvda vretiatcpd
      781 clvvkkdade vaemiarytg itmrdviddp rrrkeeieel svleqvspte igvqveeegq
      841 iepkieeaev ieelvveevt qsiikeeiqd ieepitaqkv aeveappeke ktvtptvsvv
      901 qevskepskv sipqvvsike etpseeeipp lmkegvpiav eispeevekv pveavktdtv
      961 psadkedtit pvevkqevsq eekeedvdti tikekeevve aevpmekked ealvsreeet
     1021 esrilpeede mvtaqkkeie ddllpeveek kapdeetvev dmlevmeepk eedigvellp
     1081 ekeeeeirke dvtvellpde vpkeeeveme mlpeeeevse pkeegeeeae vpteeaeett
     1141 vlvvavipee evgieaeetp vpeekveevp ieeiieveei vdkekieeip eeekvemlpe
     1201 eekvemlpee eevemlpeee kvemlpeevg eepaeekeae eevtevpvse eleicieeic
     1261 egpckeeipa eekeseevie vpvleeleic tieickrpct eevpaeekes eevievpvsd
     1321 emeipvevck eeicikkvsv pevlevpkvp evpaleevvs fpcvcfiepe vpkdkevell
     1381 pcicftepev lkdkeeeqll pcvcssepev pkvpdkeeaq tfpcvcfnep kvskvpvqke
     1441 apcdcfsile epapkaskvp vpedptklcs cffaletpea papkiptpev plvmpfvavl
     1501 ptiaeqqtep etkieitqte fsatmesiev vtqtvsetgt qtptnkftrp sqqpriitit
     1561 ksvdpeleil dqltrrltpi tepsltitta astqtekssf lqvkatyvds kppslrisei
     1621 lqslkaevah lngtpksdqe relksfhysk kpkqpelakc nccvcgkdvp ssisidprtq
     1681 rqstqrqstq rqstqrqdst vscaqppppt trktvtpytn qgmqneticp dckirklqea
     1741 lklqealklq ealklqetlk sqealksqes vksskkevkk ltsdkevcav asttprgipf
     1801 hrlkkkvdqc csakiskass kdssktsskr garnyach
//
LOCUS       XP_076545685            1461 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305538 [Osmia lignaria lignaria].
ACCESSION   XP_076545685
VERSION     XP_076545685.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689570.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1461
                     /product="uncharacterized protein LOC143305538"
                     /calculated_mol_wt=164330
     Region          <4..>59
                     /region_name="PRK10905"
                     /note="cell division protein DamX; Validated"
                     /db_xref="CDD:236792"
     Region          185..325
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          469..>582
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(486,488,490,513..515)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            486..488
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            486
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(513..516,520..523)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          712..928
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(777..782,816..817,852,854..855,924..925)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(777..782,816,854)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            817
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          943..1104
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <1071..1146
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     CDS             1..1461
                     /gene="LOC143305538"
                     /coded_by="XM_076689570.1:1..4386"
                     /db_xref="GeneID:143305538"
ORIGIN      
        1 msdkeekpak atpqtskpgs rtasptpvaa tlavptalpt eappptvekr atrssspagl
       61 smeltfkaks qmsrvemlrr imdkmpemld ettpkdiesi saqvdeihkl fqkehaylev
      121 twpasqashp ylkedyyele mgvvlevrrr lgkfrvafek lrerrgesta rrtpsklpel
      181 tlptftgrye awpafcelfa siiidnerls nverlhylrs clrseslrvi anlpstgesf
      241 piawanlkqr yenkriiiss lldklfstsp vqqrtaaayh nvaanlrdyn tslralaspe
      301 elwnctlvhv msrsldkttr eawetslgtt tefpkpekle eflttkaral ervelpaaaq
      361 ahaqakaktt gqrpaltttq aatiarasah aypcdccqed hfvisctkfr amtvperrrl
      421 vdklslcrav valsaqtqwv ndpaqpptpd qrscslatqa eltllatana qvitssathn
      481 arllvdsgse lsfvsedlve qlglqrtqst isitgiggkk stqtrgivll hlqslyntss
      541 vliqthilrt ltatlpssea pkedwphlnr lkladpdfyk prarvyssfn atifqdsdaa
      601 lrelltkfwi qeepptetts qltpeeqece nhflathgrd ttgryvarip lksntkvlgt
      661 systarsclr rtlnklsrnp eyreqyqrfm seyedlghmk kassislnds siyylphhgv
      721 fkpdsettkl rvvfngsspt ttglsvddim htganlllni tdvllwirhh kcifatditk
      781 myrqvrvhed dwnlqripwi deesnevpyt lttvtygtka apflavrall qlaedegqry
      841 llavpsikha ryvdnifgga dsldslieia sqltdlcnag gfplakwhsn easlletivp
      901 cnnsqgtsis lddcntktlg lrwntvndsf tfttkshhnl nftkrlvlse vvqifdplgf
      961 lspviirakv llqelwllnl kwddplpshv ttrwiaired ltslarlsip rwfntynnst
     1021 velhgfsdas qlamaaviyi tvfspftdlk vtslvcsktk vaplkkltip rleltaalif
     1081 akllkyvqan lklniaaihl wtdsqvtltw ikshasrwkd yvrnrvtqiq eltqtatwmh
     1141 vpgtsnpadc asrgltttql eqhelwwrgp pwllqsqesw pvqrnasddt clqecrpgis
     1201 hvltcqkidy ywdlihkyss lqklfhvtsv cyrfisklrn ttastrlsnt padlekakif
     1261 wikatqsayf shelkmmvnn qtlpsshafn rltafiddqg virvggrlsn aqltyedsal
     1321 ksmfvegtqd nqrlaklfvd dhttwsfnpp aaphmggkwe avvksvkyhl rrtqsqhcls
     1381 hllnrptshr epggisynkn ynnsgsagql nisngcnqsl sgstlpttst ldlwcclqts
     1441 ashhasglll ecsqftldqm d
//
LOCUS       XP_076545686             404 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610930 [Osmia lignaria lignaria].
ACCESSION   XP_076545686
VERSION     XP_076545686.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689571.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="uncharacterized protein LOC117610930"
                     /calculated_mol_wt=46362
     Region          <309..339
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     CDS             1..404
                     /gene="LOC117610930"
                     /coded_by="XM_076689571.1:166..1380"
                     /db_xref="GeneID:117610930"
ORIGIN      
        1 mtasgpaaqe nvlrqlldrm qrmeeginqq lttlgeenqn rakeiqniae qlklvtlted
       61 astisritrr sggdqdnqqe demhtarqpp qnstirpnel mkavdairti etlrgrddvg
      121 vedfiksvry arsncsektl llklilieki tenakrsiry tsietyeely evlrqnvsip
      181 ntvsscrnkl qqirqgamet vqsynlrfrq qlneliytvq nkhqnpisrr iaieeeetea
      241 trtyvlnlrr digmlvipsk pkllieaqtm aadmelwtrd anramerrqv psripppkqq
      301 gqrpntssdr pnhsiplkct kcgrlghtsd kcyarnfqva nqgkippprv nnlnfqedkv
      361 tettsestap penyykfaae fqecresedy swtqeqests ssdq
//
LOCUS       XP_076545687             616 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305540 [Osmia lignaria lignaria].
ACCESSION   XP_076545687
VERSION     XP_076545687.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689572.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..616
                     /product="uncharacterized protein LOC143305540"
                     /calculated_mol_wt=69277
     Region          22..233
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(86..91,126..127,162,164..165,229..230)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(86..91,126,164)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            127
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          250..410
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..616
                     /gene="LOC143305540"
                     /coded_by="XM_076689572.1:1..1851"
                     /db_xref="GeneID:143305540"
ORIGIN      
        1 meyltlqhmq gapeeadkgp ayylphhavr krhdpsaklr vvfnasfcta tgqslndcla
       61 tgqklqadlw mvltrwrlfr vvfttdivkm frqirvhsed tkwqsilwra tpdervqdfr
      121 lttvtygtac apflalrvla qlandessrf prgasvvrrh tyvddiltga ddvsetlalk
      181 revvaifkag gfelskwasn ipeiqeadss ntrlfqewsg istlgvnwnp rddafslrva
      241 aaelvrectt krsilseiag lfdplgwaap vlvvakilmq dlwilgadwd qqlpenvctm
      301 wqrfrgslqq ldtlkiprwt fassepstqm elhgfcdasq rayaaavylr vnnngsvtts
      361 llvaktkvap vktitipkle lcgatllskl lirvkeginm sgptiawtds svtlhwirgh
      421 asqwkpfvah rvsdiqheip adnwrhirsp dnpadlatrg mspaelvdsd iwwhgpkwlt
      481 eqpefwpatf pesnegieae rrgatvhvvk ekfgenflcr fsslshllsv iaqcfrfasl
      541 srgqpcekgf vkaaerthaf lavlrfsqht sfleeieqlq rhgelrkasp lspcrpfldd
      601 qgllrlggrl enaalp
//
LOCUS       XP_076545688             778 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305541 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545688
VERSION     XP_076545688.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689573.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 41% of CDS bases
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..778
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305541"
                     /calculated_mol_wt=86309
     Region          269..330
                     /region_name="HTH_Tnp_Tc5"
                     /note="Tc5 transposase DNA-binding domain; pfam03221"
                     /db_xref="CDD:460850"
     Region          411..>535
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     CDS             1..778
                     /gene="LOC143305541"
                     /coded_by="XM_076689573.1:1..2337"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 1 codon;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:143305541"
ORIGIN      
        1 masgngklqs cqyqstfdrt lthrgskvvt vqrqsmnkvt hsytaqyalt lsgkllpvvf
       61 vclqeasdge dkkpeeaell lqlqqllqqq ssqmqqnkte latlrqqiqe lkaaapaaaa
      121 tasaaapaav sglsgvsvps sralcagtan rqnsgcsgna aerqggcggk qravtvaasv
      181 aaavavlaaa ggtfekkeek tetvkrekck nkylrftedd linalqavkn gmpmrvaskq
      241 fkvpystvra khkgiyvigk ksgpptiltk eeetmlmdwi lsiankgfpf tkdvlldsvq
      301 llikkmnkqn pftngrpgrh wfeafmkrhp qlslrtpqnl cerrgqvseg kirewfkeie
      361 dylksnnlld idssrifncd esafflnpks nkvitnkksk giynlvgand kecittlitg
      421 naagellppm imfsyqrlps siidkvprdw sigksdngwm tgesfyeymt nvfypwcikn
      481 xikfpiimyl dghsshatma lsdfcvehei eiislylnst hitqpmdval frplkeawxk
      541 ctdwrmqngg kavekqnfap vlqtalhsln vknilqqgfi acglaplsad avnyekllnh
      601 dqllqqkdlt nmnsytsshn nyectltfie nyiddktvql fkvaegrdgk wygeekyead
      661 tisnheivni efinekqags sncdgniidn nleyvdlssi sedilennld iskyeivsyd
      721 stkiytsaqi tqlnqkktnn nsdiaeqpdi lshgvivdyn sqvtdenacp nkhnkgcg
//
LOCUS       XP_034184989            3322 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606542 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184989
VERSION     XP_034184989.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184989.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3322
                     /product="uncharacterized protein LOC117606542 isoform X1"
                     /calculated_mol_wt=363010
     Region          60..112
                     /region_name="FYVE_Slp3_4_5"
                     /note="FYVE-related domain found in the synaptotagmin-like
                     proteins 3, 4, 5; cd15747"
                     /db_xref="CDD:277286"
     Region          <244..713
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2135..>2321
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     Region          2998..3120
                     /region_name="C2A_SLP"
                     /note="C2 domain first repeat present in
                     Synaptotagmin-like proteins; cd08521"
                     /db_xref="CDD:176056"
     Region          3134..3310
                     /region_name="C2B_SLP_1-2-3-4"
                     /note="C2 domain second repeat present in
                     Synaptotagmin-like proteins 1-4; cd04020"
                     /db_xref="CDD:175987"
     CDS             1..3322
                     /gene="LOC117606542"
                     /coded_by="XM_034329098.2:46..10014"
                     /db_xref="GeneID:117606542"
ORIGIN      
        1 mesryipggt ptrctytdai laparkdvai fiawrlkadl qylrrkgllk psgelsyqgr
       61 kcarcgapfg rfyntgapct rcrhrvcrqc rievrsptdn kdvewicnic ykiaelhivt
      121 gkwtyenqss krsiggkasf pveilkhsir rawtinsppt rwsaarkspe lriyrslpsr
      181 hqeyrdiink neivkssvdr dasnsdpfgk etnedcngvv trnetispdk sgsradiner
      241 tnskriekls hkqeedvpsp daikskktnr stattnsktr neeqeetsny qepstprisl
      301 lkpprilskf ispevkssqt khgerksnip ifrrsskefe dirspqespk rslipqryrg
      361 stddlrrsre disvpslipk llsprgkedr lkrqdskddv rcgtksgrke pkdetrnwsg
      421 nkeesrlgir ifrrdnsred vgresansna naaakgkqag keekqertee rvgvsatsks
      481 kstvheqsrt kvsvnkespd gklrldeesp rveqddrvrd eaagkedtml dvvvtdeill
      541 keqenvkkge eregkgfaea edsmlddtea sendkntdsm kdtsesfaen adadvaafra
      601 ferkavtdva ygkksddfqv irrkfskeef snpddtddah nkrcsshlsp easplstrss
      661 ryspresese ptpalprktg dsssasqrir daitrtrres nssaryessg sesirlsegl
      721 hgyaelarkv klfdgngsga vpsaigsdev vqrdsatsag edeshddnhg rtapvssqgr
      781 sifrrrrqfd aqgsrrrgrs hartccseel qsfqalhvig rdgrqlsptg nqaqdqplvd
      841 yrrlvfissd sssgreedda dsscssssfl nglprgsshs qsledcdwdy fesgtlpvpl
      901 ppgvpvtvat nnvgtdtnns qdwsccpgrg ttaclacggs rstnvlpvpv pipvpvpypv
      961 pvpaslwtgd faaaassmis rsddahaepg tlrlrgdpaa pwfawhprfn qplhgtrldp
     1021 rlasafdpat ctfrpdqvla srlygptiet saipttealq qrlsglqinl nendeagasa
     1081 asksaetfqd dsaldieges kfreqsslrg gleetkaekn qenneetnet kegeedcqds
     1141 vsvsfesrrg qrmyrhedsg sssgrssads sdldddsssh rfsrifvvnd dsltsdndie
     1201 dngdeddsds aaengitlkr vtalpdeepv vlqyvrflse dsnfesasgg rtensadgss
     1261 egrdkmvhee nanmnekrkv rgrrssvern ndkritvslq hrempeemyi asdndysenq
     1321 ielksiktld pdsiepvsln nasnnnskdd dghnnffltd gwdsniipds leiscqkmln
     1381 stslspdnvl eatnfrvstp lmahdqtngn tccetradns tplsqiitne iidrdddnve
     1441 hditptdlns nlsvaasvss gsesftsnet natgsevssd elheyevtpa nisspyegke
     1501 flfpatngeq ldktieersn ltddtpisfk dtdycgesav ntsgrakwnv qyertdveqs
     1561 ldksnnggvv svrpesdsms itaeddqsvt cqagnpvagg adgavddped ghgaiesksa
     1621 dcdvgdmisn kiqemfksag rvesahdggt gtvrgdspts gqvvgevygs ggegsgidag
     1681 sggadggcse egiegestrl rsverrpstg kengfppaas tkdsirdvlq laptvqnnni
     1741 gnnnsnkyrs lvmitqeasy qpvsvitsdt ttllqpdevh tasqglagyf qlaleavseq
     1801 phrkngqgpr rplmvkrlas tttnhseaev dsglsvgsas esedvsvkna akissrqnen
     1861 nedsgskerq vnehregnra rafsqssden sssggvvile egladddswv eevshdedea
     1921 ggttateess edeannlgdr eeelrgyhrq aidftlhtiv eesceeseae pslatgspsk
     1981 kqrppsasel ekyfffglgg dgnnssmgsr evdslsetss iysegleslg qdetsgtgqs
     2041 qersnsgegf lnssrlekyf lseflgfdqd rrdsdgsvgs dsegrpspea qrrkklvrar
     2101 gtgrshsssl dnllaltqss qnlssqnslq dlslnqdsqd tqsegsstet dgaddqtaaf
     2161 gtdgqfdtvk rkkkkprnlg tqsprvpddr nktpepnrem vlvgevvmen eaealnseds
     2221 eraktpqpes vlssspsvtv nnltsstlld ssrniqmssg esmsynqksk qqsrdsgfvg
     2281 scddllqhqk lpddvqsnte vsqdsakdrs siennknvqd snqmttgvag vekgnvtkvd
     2341 halnhttlph lpnaslsrkd sfnnwssdee tnlmmskmrq ffktmvansn gqnqnatnan
     2401 ssaaspapsp rlpgytskar lcsqtrtkpp qlvyfenelt rlmktvpgir deqvreivey
     2461 lssedtwsds ydssdytssd legaaggrrs alqeqisqsc qqiiskfdtt sgdnlldkek
     2521 edktgptavq apclgrdtaf vyqklvqsft kmagdgvssd anstphsspp liakvmhhig
     2581 srlvalmhev seggpavqph stswrrysqh hrtpssastt edddstsdst napssahlql
     2641 prskshdpll linthpaast gqegeereas dyerfswrgs fesalmaads rtklsllacs
     2701 gdsvgasasa salamaakrr sagdllfnpk sfsreqldrv rscgsigggs ggatsgcggs
     2761 veeriwsnrr rssvpdantr gesgasagde dtedeleysg esrattlprg mqsaitaatn
     2821 slprlpasta pagltttstt psltmhkahs vyhflpqhsg vksaryrppg farnsnvaga
     2881 iggpkravsa pglqvsgsqs sagkrdnsrt rrqqamsltp ddgsslseac stpiigagsr
     2941 sgssqtvidv ddmdsglhsg hlatraslss lgarsdsmas vysgagegrc crsvvvtgev
     3001 efalqydykh ltfevhvtkc knlapvdvkr krsdpyvkvy llpdksksgk rktkvkkhtl
     3061 npefnetlkf hmslsgletr tlwltvwhsd mfgrndflge vrmplenkif ddptpkwypl
     3121 qertepfddp vaykgevivg lkfvppdptq qerdretgsd rgiskskknw srgalhvlvk
     3181 earnlqtrak nsgtcdpfck syllpdkgrs gkqktgvvrr sggspvwght fiykdvslqe
     3241 laergleltv wdhdriasne flggvrfnlg tgkhygkpvd wmdatgrels lwqsmlerpn
     3301 fwvegavtlr pnlhnhgkng ns
//
LOCUS       XP_034184990            3318 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606542 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184990
VERSION     XP_034184990.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329099.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184990.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3318
                     /product="uncharacterized protein LOC117606542 isoform X2"
                     /calculated_mol_wt=362723
     Region          60..112
                     /region_name="FYVE_Slp3_4_5"
                     /note="FYVE-related domain found in the synaptotagmin-like
                     proteins 3, 4, 5; cd15747"
                     /db_xref="CDD:277286"
     Region          <244..713
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2135..>2321
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     Region          2994..3116
                     /region_name="C2A_SLP"
                     /note="C2 domain first repeat present in
                     Synaptotagmin-like proteins; cd08521"
                     /db_xref="CDD:176056"
     Region          3130..3306
                     /region_name="C2B_SLP_1-2-3-4"
                     /note="C2 domain second repeat present in
                     Synaptotagmin-like proteins 1-4; cd04020"
                     /db_xref="CDD:175987"
     CDS             1..3318
                     /gene="LOC117606542"
                     /coded_by="XM_034329099.2:47..10003"
                     /db_xref="GeneID:117606542"
ORIGIN      
        1 mesryipggt ptrctytdai laparkdvai fiawrlkadl qylrrkgllk psgelsyqgr
       61 kcarcgapfg rfyntgapct rcrhrvcrqc rievrsptdn kdvewicnic ykiaelhivt
      121 gkwtyenqss krsiggkasf pveilkhsir rawtinsppt rwsaarkspe lriyrslpsr
      181 hqeyrdiink neivkssvdr dasnsdpfgk etnedcngvv trnetispdk sgsradiner
      241 tnskriekls hkqeedvpsp daikskktnr stattnsktr neeqeetsny qepstprisl
      301 lkpprilskf ispevkssqt khgerksnip ifrrsskefe dirspqespk rslipqryrg
      361 stddlrrsre disvpslipk llsprgkedr lkrqdskddv rcgtksgrke pkdetrnwsg
      421 nkeesrlgir ifrrdnsred vgresansna naaakgkqag keekqertee rvgvsatsks
      481 kstvheqsrt kvsvnkespd gklrldeesp rveqddrvrd eaagkedtml dvvvtdeill
      541 keqenvkkge eregkgfaea edsmlddtea sendkntdsm kdtsesfaen adadvaafra
      601 ferkavtdva ygkksddfqv irrkfskeef snpddtddah nkrcsshlsp easplstrss
      661 ryspresese ptpalprktg dsssasqrir daitrtrres nssaryessg sesirlsegl
      721 hgyaelarkv klfdgngsga vpsaigsdev vqrdsatsag edeshddnhg rtapvssqgr
      781 sifrrrrqfd aqgsrrrgrs hartccseel qsfqalhvig rdgrqlsptg nqaqdqplvd
      841 yrrlvfissd sssgreedda dsscssssfl nglprgsshs qsledcdwdy fesgtlpvpl
      901 ppgvpvtvat nnvgtdtnns qdwsccpgrg ttaclacggs rstnvlpvpv pipvpvpypv
      961 pvpaslwtgd faaaassmis rsddahaepg tlrlrgdpaa pwfawhprfn qplhgtrldp
     1021 rlasafdpat ctfrpdqvla srlygptiet saipttealq qrlsglqinl nendeagasa
     1081 asksaetfqd dsaldieges kfreqsslrg gleetkaekn qenneetnet kegeedcqds
     1141 vsvsfesrrg qrmyrhedsg sssgrssads sdldddsssh rfsrifvvnd dsltsdndie
     1201 dngdeddsds aaengitlkr vtalpdeepv vlqyvrflse dsnfesasgg rtensadgss
     1261 egrdkmvhee nanmnekrkv rgrrssvern ndkritvslq hrempeemyi asdndysenq
     1321 ielksiktld pdsiepvsln nasnnnskdd dghnnffltd gwdsniipds leiscqkmln
     1381 stslspdnvl eatnfrvstp lmahdqtngn tccetradns tplsqiitne iidrdddnve
     1441 hditptdlns nlsvaasvss gsesftsnet natgsevssd elheyevtpa nisspyegke
     1501 flfpatngeq ldktieersn ltddtpisfk dtdycgesav ntsgrakwnv qyertdveqs
     1561 ldksnnggvv svrpesdsms itaeddqsvt cqagnpvagg adgavddped ghgaiesksa
     1621 dcdvgdmisn kiqemfksag rvesahdggt gtvrgdspts gqvvgevygs ggegsgidag
     1681 sggadggcse egiegestrl rsverrpstg kengfppaas tkdsirdvlq laptvqnnni
     1741 gnnnsnkyrs lvmitqeasy qpvsvitsdt ttllqpdevh tasqglagyf qlaleavseq
     1801 phrkngqgpr rplmvkrlas tttnhseaev dsglsvgsas esedvsvkna akissrqnen
     1861 nedsgskerq vnehregnra rafsqssden sssggvvile egladddswv eevshdedea
     1921 ggttateess edeannlgdr eeelrgyhrq aidftlhtiv eesceeseae pslatgspsk
     1981 kqrppsasel ekyfffglgg dgnnssmgsr evdslsetss iysegleslg qdetsgtgqs
     2041 qersnsgegf lnssrlekyf lseflgfdqd rrdsdgsvgs dsegrpspea qrrkklvrar
     2101 gtgrshsssl dnllaltqss qnlssqnslq dlslnqdsqd tqsegsstet dgaddqtaaf
     2161 gtdgqfdtvk rkkkkprnlg tqsprvpddr nktpepnrem vlvgevvmen eaealnseds
     2221 eraktpqpes vlssspsvtv nnltsstlld ssrniqmssg esmsynqksk qqsrdsgfvg
     2281 scddllqhqk lpddvqsnte vsqdsakdrs siennknvqd snqmttgvag vekgnvtkvd
     2341 halnhttlph lpnaslsrkd sfnnwssdee tnlmmskmrq ffktmvansn gqnqnatnan
     2401 ssaaspapsp rlpgytskar lcsqtrtkpp qlvyfenelt rlmktvpgir deqvreivey
     2461 lssedtwsds ydssdytssd legaaggrrs alqeqisqsc qqiiskfdtt sgdnlldkek
     2521 edktgptavq apclgrdtaf vyqklvqsft kmagdgvssd anstphsspp liakvmhhig
     2581 srlvalmhev seggpavqph stswrrysqh hrtpssastt edddstsdst napssahlql
     2641 prskshdpll linthpaast gqegeereas dyerfswrgs fesalmaads rtklsllacs
     2701 gdsvgasasa salamaakrr sagdllfnpk sfsreqldrv rscgsigggs ggatsgcggs
     2761 veeriwsnrr rssvpdantr gesgdedted eleysgesra ttlprgmqsa itaatnslpr
     2821 lpastapagl tttsttpslt mhkahsvyhf lpqhsgvksa ryrppgfarn snvagaiggp
     2881 kravsapglq vsgsqssagk rdnsrtrrqq amsltpddgs slseacstpi igagsrsgss
     2941 qtvidvddmd sglhsghlat raslsslgar sdsmasvysg agegrccrsv vvtgevefal
     3001 qydykhltfe vhvtkcknla pvdvkrkrsd pyvkvyllpd ksksgkrktk vkkhtlnpef
     3061 netlkfhmsl sgletrtlwl tvwhsdmfgr ndflgevrmp lenkifddpt pkwyplqert
     3121 epfddpvayk gevivglkfv ppdptqqerd retgsdrgis kskknwsrga lhvlvkearn
     3181 lqtraknsgt cdpfcksyll pdkgrsgkqk tgvvrrsggs pvwghtfiyk dvslqelaer
     3241 gleltvwdhd riasneflgg vrfnlgtgkh ygkpvdwmda tgrelslwqs mlerpnfwve
     3301 gavtlrpnlh nhgkngns
//
LOCUS       XP_034184992            3309 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606542 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184992
VERSION     XP_034184992.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329101.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184992.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3309
                     /product="uncharacterized protein LOC117606542 isoform X3"
                     /calculated_mol_wt=361520
     Region          47..99
                     /region_name="FYVE_Slp3_4_5"
                     /note="FYVE-related domain found in the synaptotagmin-like
                     proteins 3, 4, 5; cd15747"
                     /db_xref="CDD:277286"
     Region          <231..700
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2122..>2308
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     Region          2985..3107
                     /region_name="C2A_SLP"
                     /note="C2 domain first repeat present in
                     Synaptotagmin-like proteins; cd08521"
                     /db_xref="CDD:176056"
     Region          3121..3297
                     /region_name="C2B_SLP_1-2-3-4"
                     /note="C2 domain second repeat present in
                     Synaptotagmin-like proteins 1-4; cd04020"
                     /db_xref="CDD:175987"
     CDS             1..3309
                     /gene="LOC117606542"
                     /coded_by="XM_034329101.2:80..10009"
                     /db_xref="GeneID:117606542"
ORIGIN      
        1 msttdvfria qqaptgeeeq grlkadlqyl rrkgllkpsg elsyqgrkca rcgapfgrfy
       61 ntgapctrcr hrvcrqcrie vrsptdnkdv ewicnicyki aelhivtgkw tyenqsskrs
      121 iggkasfpve ilkhsirraw tinspptrws aarkspelri yrslpsrhqe yrdiinknei
      181 vkssvdrdas nsdpfgketn edcngvvtrn etispdksgs radinertns krieklshkq
      241 eedvpspdai kskktnrsta ttnsktrnee qeetsnyqep stprisllkp prilskfisp
      301 evkssqtkhg erksnipifr rsskefedir spqespkrsl ipqryrgstd dlrrsredis
      361 vpslipklls prgkedrlkr qdskddvrcg tksgrkepkd etrnwsgnke esrlgirifr
      421 rdnsredvgr esansnanaa akgkqagkee kqerteervg vsatskskst vheqsrtkvs
      481 vnkespdgkl rldeesprve qddrvrdeaa gkedtmldvv vtdeillkeq envkkgeere
      541 gkgfaeaeds mlddteasen dkntdsmkdt sesfaenada dvaafrafer kavtdvaygk
      601 ksddfqvirr kfskeefsnp ddtddahnkr csshlspeas plstrssrys preseseptp
      661 alprktgdss sasqrirdai trtrresnss aryessgses irlseglhgy aelarkvklf
      721 dgngsgavps aigsdevvqr dsatsagede shddnhgrta pvssqgrsif rrrrqfdaqg
      781 srrrgrshar tccseelqsf qalhvigrdg rqlsptgnqa qdqplvdyrr lvfissdsss
      841 greeddadss cssssflngl prgsshsqsl edcdwdyfes gtlpvplppg vpvtvatnnv
      901 gtdtnnsqdw sccpgrgtta clacggsrst nvlpvpvpip vpvpypvpvp aslwtgdfaa
      961 aassmisrsd dahaepgtlr lrgdpaapwf awhprfnqpl hgtrldprla safdpatctf
     1021 rpdqvlasrl ygptietsai pttealqqrl sglqinlnen deagasaask saetfqddsa
     1081 ldiegeskfr eqsslrggle etkaeknqen neetnetkeg eedcqdsvsv sfesrrgqrm
     1141 yrhedsgsss grssadssdl dddssshrfs rifvvnddsl tsdndiedng deddsdsaae
     1201 ngitlkrvta lpdeepvvlq yvrflsedsn fesasggrte nsadgssegr dkmvheenan
     1261 mnekrkvrgr rssvernndk ritvslqhre mpeemyiasd ndysenqiel ksiktldpds
     1321 iepvslnnas nnnskdddgh nnffltdgwd sniipdslei scqkmlnsts lspdnvleat
     1381 nfrvstplma hdqtngntcc etradnstpl sqiitneiid rdddnvehdi tptdlnsnls
     1441 vaasvssgse sftsnetnat gsevssdelh eyevtpanis spyegkeflf patngeqldk
     1501 tieersnltd dtpisfkdtd ycgesavnts grakwnvqye rtdveqsldk snnggvvsvr
     1561 pesdsmsita eddqsvtcqa gnpvaggadg avddpedghg aiesksadcd vgdmisnkiq
     1621 emfksagrve sahdggtgtv rgdsptsgqv vgevygsgge gsgidagsgg adggcseegi
     1681 egestrlrsv errpstgken gfppaastkd sirdvlqlap tvqnnnignn nsnkyrslvm
     1741 itqeasyqpv svitsdtttl lqpdevhtas qglagyfqla leavseqphr kngqgprrpl
     1801 mvkrlasttt nhseaevdsg lsvgsasese dvsvknaaki ssrqnenned sgskerqvne
     1861 hregnraraf sqssdensss ggvvileegl adddswveev shdedeaggt tateessede
     1921 annlgdreee lrgyhrqaid ftlhtivees ceeseaepsl atgspskkqr ppsaseleky
     1981 fffglggdgn nssmgsrevd slsetssiys egleslgqde tsgtgqsqer snsgegflns
     2041 srlekyflse flgfdqdrrd sdgsvgsdse grpspeaqrr kklvrargtg rshsssldnl
     2101 laltqssqnl ssqnslqdls lnqdsqdtqs egsstetdga ddqtaafgtd gqfdtvkrkk
     2161 kkprnlgtqs prvpddrnkt pepnremvlv gevvmeneae alnsedsera ktpqpesvls
     2221 sspsvtvnnl tsstlldssr niqmssgesm synqkskqqs rdsgfvgscd dllqhqklpd
     2281 dvqsntevsq dsakdrssie nnknvqdsnq mttgvagvek gnvtkvdhal nhttlphlpn
     2341 aslsrkdsfn nwssdeetnl mmskmrqffk tmvansngqn qnatnanssa aspapsprlp
     2401 gytskarlcs qtrtkppqlv yfeneltrlm ktvpgirdeq vreiveylss edtwsdsyds
     2461 sdytssdleg aaggrrsalq eqisqscqqi iskfdttsgd nlldkekedk tgptavqapc
     2521 lgrdtafvyq klvqsftkma gdgvssdans tphsspplia kvmhhigsrl valmhevseg
     2581 gpavqphsts wrrysqhhrt pssasttedd dstsdstnap ssahlqlprs kshdplllin
     2641 thpaastgqe geereasdye rfswrgsfes almaadsrtk lsllacsgds vgasasasal
     2701 amaakrrsag dllfnpksfs reqldrvrsc gsigggsgga tsgcggsvee riwsnrrrss
     2761 vpdantrges gasagdedte deleysgesr attlprgmqs aitaatnslp rlpastapag
     2821 ltttsttpsl tmhkahsvyh flpqhsgvks aryrppgfar nsnvagaigg pkravsapgl
     2881 qvsgsqssag krdnsrtrrq qamsltpddg sslseacstp iigagsrsgs sqtvidvddm
     2941 dsglhsghla traslsslga rsdsmasvys gagegrccrs vvvtgevefa lqydykhltf
     3001 evhvtkcknl apvdvkrkrs dpyvkvyllp dksksgkrkt kvkkhtlnpe fnetlkfhms
     3061 lsgletrtlw ltvwhsdmfg rndflgevrm plenkifddp tpkwyplqer tepfddpvay
     3121 kgevivglkf vppdptqqer dretgsdrgi skskknwsrg alhvlvkear nlqtraknsg
     3181 tcdpfcksyl lpdkgrsgkq ktgvvrrsgg spvwghtfiy kdvslqelae rgleltvwdh
     3241 driasneflg gvrfnlgtgk hygkpvdwmd atgrelslwq smlerpnfwv egavtlrpnl
     3301 hnhgkngns
//
LOCUS       XP_076545689            1041 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545689
VERSION     XP_076545689.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689574.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1041
                     /product="uncharacterized protein LOC117606543 isoform X1"
                     /calculated_mol_wt=122271
     Region          <317..584
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <777..915
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1041
                     /gene="LOC117606543"
                     /coded_by="XM_076689574.1:219..3344"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mspplplslg mpeegempae aflqwrmqpg vtvvlatvwl yanvvgtasl nssrfsldqy
       61 splapqhely qrvpigsrrs sststasplq sfktnyldkd slhskskgns ggddddfspl
      121 vedkqrrkkf teqkidgggt kdseqpslal seledpfiad dtfqdqgpgs meiykldllr
      181 lyplnhvplt ryllkgkgkt pkktsqadwk akttdyksts pkmfykqsns fddlstevpp
      241 nsdpfsiggv pelqvgcegl aatkykqkrs idsggrdkap iaamdtwpgv tpisldmydf
      301 ssveeeedem delvrlkkle tttkgikqnr gdmeatrysd sddkgrpekl pvrgdlgksn
      361 gqnstggdqk tagegsnsdi akreafeeee aksadslvpa hqrrrilwld dsvadeqlrt
      421 egrskrqiwg fgedegvvss delqtenqrr rqdyerrrke eerrrleear rrghvenrtn
      481 severikqey erslqertrq eeerqrrlqq earrtssrwq speehrerpq elvasprrye
      541 syphtstyde errhqeaeam rrrdweterr rqqeldrrhl eqrrrewmlk rrqeeederr
      601 rqqarnepsn llyamraasn esgpyyvdpe rrfreqqere reeklkeymq rnrpinpadr
      661 qreeeirrrq deerrrieee rklqeyirrn qpvhvtsgng ssvdqrkkmd dwsraynwsr
      721 ypgqgngrrn hgpsaptnid prsfveerrr keasrrteed rirererlee eqlrkealrr
      781 eeearrlqsr ryeeqrkrle aarrdadrrr kelmeeqarr sqagrtrsen trhgyddrar
      841 leelrrrqdt slipanlvln esrrtmeqdr qrqeqdrqry eqerwrleae rrrqedsrak
      901 qgreheqrrr qeearlnalp vsariivhpa qssssprlss rtgfdgdidf pgvnpyrrgv
      961 evpnfpaaat rrppvqsppa cvwavvqccp fntnrlvtcf esmgcpgvnw dpnpcrgsit
     1021 kaarqqitkf yaeseeenrg l
//
LOCUS       XP_076545690            1041 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545690
VERSION     XP_076545690.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689575.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1041
                     /product="uncharacterized protein LOC117606543 isoform X1"
                     /calculated_mol_wt=122271
     Region          <317..584
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <777..915
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1041
                     /gene="LOC117606543"
                     /coded_by="XM_076689575.1:219..3344"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mspplplslg mpeegempae aflqwrmqpg vtvvlatvwl yanvvgtasl nssrfsldqy
       61 splapqhely qrvpigsrrs sststasplq sfktnyldkd slhskskgns ggddddfspl
      121 vedkqrrkkf teqkidgggt kdseqpslal seledpfiad dtfqdqgpgs meiykldllr
      181 lyplnhvplt ryllkgkgkt pkktsqadwk akttdyksts pkmfykqsns fddlstevpp
      241 nsdpfsiggv pelqvgcegl aatkykqkrs idsggrdkap iaamdtwpgv tpisldmydf
      301 ssveeeedem delvrlkkle tttkgikqnr gdmeatrysd sddkgrpekl pvrgdlgksn
      361 gqnstggdqk tagegsnsdi akreafeeee aksadslvpa hqrrrilwld dsvadeqlrt
      421 egrskrqiwg fgedegvvss delqtenqrr rqdyerrrke eerrrleear rrghvenrtn
      481 severikqey erslqertrq eeerqrrlqq earrtssrwq speehrerpq elvasprrye
      541 syphtstyde errhqeaeam rrrdweterr rqqeldrrhl eqrrrewmlk rrqeeederr
      601 rqqarnepsn llyamraasn esgpyyvdpe rrfreqqere reeklkeymq rnrpinpadr
      661 qreeeirrrq deerrrieee rklqeyirrn qpvhvtsgng ssvdqrkkmd dwsraynwsr
      721 ypgqgngrrn hgpsaptnid prsfveerrr keasrrteed rirererlee eqlrkealrr
      781 eeearrlqsr ryeeqrkrle aarrdadrrr kelmeeqarr sqagrtrsen trhgyddrar
      841 leelrrrqdt slipanlvln esrrtmeqdr qrqeqdrqry eqerwrleae rrrqedsrak
      901 qgreheqrrr qeearlnalp vsariivhpa qssssprlss rtgfdgdidf pgvnpyrrgv
      961 evpnfpaaat rrppvqsppa cvwavvqccp fntnrlvtcf esmgcpgvnw dpnpcrgsit
     1021 kaarqqitkf yaeseeenrg l
//
LOCUS       XP_076545691            1041 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545691
VERSION     XP_076545691.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689576.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1041
                     /product="uncharacterized protein LOC117606543 isoform X1"
                     /calculated_mol_wt=122271
     Region          <317..584
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <777..915
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1041
                     /gene="LOC117606543"
                     /coded_by="XM_076689576.1:219..3344"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mspplplslg mpeegempae aflqwrmqpg vtvvlatvwl yanvvgtasl nssrfsldqy
       61 splapqhely qrvpigsrrs sststasplq sfktnyldkd slhskskgns ggddddfspl
      121 vedkqrrkkf teqkidgggt kdseqpslal seledpfiad dtfqdqgpgs meiykldllr
      181 lyplnhvplt ryllkgkgkt pkktsqadwk akttdyksts pkmfykqsns fddlstevpp
      241 nsdpfsiggv pelqvgcegl aatkykqkrs idsggrdkap iaamdtwpgv tpisldmydf
      301 ssveeeedem delvrlkkle tttkgikqnr gdmeatrysd sddkgrpekl pvrgdlgksn
      361 gqnstggdqk tagegsnsdi akreafeeee aksadslvpa hqrrrilwld dsvadeqlrt
      421 egrskrqiwg fgedegvvss delqtenqrr rqdyerrrke eerrrleear rrghvenrtn
      481 severikqey erslqertrq eeerqrrlqq earrtssrwq speehrerpq elvasprrye
      541 syphtstyde errhqeaeam rrrdweterr rqqeldrrhl eqrrrewmlk rrqeeederr
      601 rqqarnepsn llyamraasn esgpyyvdpe rrfreqqere reeklkeymq rnrpinpadr
      661 qreeeirrrq deerrrieee rklqeyirrn qpvhvtsgng ssvdqrkkmd dwsraynwsr
      721 ypgqgngrrn hgpsaptnid prsfveerrr keasrrteed rirererlee eqlrkealrr
      781 eeearrlqsr ryeeqrkrle aarrdadrrr kelmeeqarr sqagrtrsen trhgyddrar
      841 leelrrrqdt slipanlvln esrrtmeqdr qrqeqdrqry eqerwrleae rrrqedsrak
      901 qgreheqrrr qeearlnalp vsariivhpa qssssprlss rtgfdgdidf pgvnpyrrgv
      961 evpnfpaaat rrppvqsppa cvwavvqccp fntnrlvtcf esmgcpgvnw dpnpcrgsit
     1021 kaarqqitkf yaeseeenrg l
//
LOCUS       XP_076545692            1041 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545692
VERSION     XP_076545692.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689577.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1041
                     /product="uncharacterized protein LOC117606543 isoform X1"
                     /calculated_mol_wt=122271
     Region          <317..584
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <777..915
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1041
                     /gene="LOC117606543"
                     /coded_by="XM_076689577.1:219..3344"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mspplplslg mpeegempae aflqwrmqpg vtvvlatvwl yanvvgtasl nssrfsldqy
       61 splapqhely qrvpigsrrs sststasplq sfktnyldkd slhskskgns ggddddfspl
      121 vedkqrrkkf teqkidgggt kdseqpslal seledpfiad dtfqdqgpgs meiykldllr
      181 lyplnhvplt ryllkgkgkt pkktsqadwk akttdyksts pkmfykqsns fddlstevpp
      241 nsdpfsiggv pelqvgcegl aatkykqkrs idsggrdkap iaamdtwpgv tpisldmydf
      301 ssveeeedem delvrlkkle tttkgikqnr gdmeatrysd sddkgrpekl pvrgdlgksn
      361 gqnstggdqk tagegsnsdi akreafeeee aksadslvpa hqrrrilwld dsvadeqlrt
      421 egrskrqiwg fgedegvvss delqtenqrr rqdyerrrke eerrrleear rrghvenrtn
      481 severikqey erslqertrq eeerqrrlqq earrtssrwq speehrerpq elvasprrye
      541 syphtstyde errhqeaeam rrrdweterr rqqeldrrhl eqrrrewmlk rrqeeederr
      601 rqqarnepsn llyamraasn esgpyyvdpe rrfreqqere reeklkeymq rnrpinpadr
      661 qreeeirrrq deerrrieee rklqeyirrn qpvhvtsgng ssvdqrkkmd dwsraynwsr
      721 ypgqgngrrn hgpsaptnid prsfveerrr keasrrteed rirererlee eqlrkealrr
      781 eeearrlqsr ryeeqrkrle aarrdadrrr kelmeeqarr sqagrtrsen trhgyddrar
      841 leelrrrqdt slipanlvln esrrtmeqdr qrqeqdrqry eqerwrleae rrrqedsrak
      901 qgreheqrrr qeearlnalp vsariivhpa qssssprlss rtgfdgdidf pgvnpyrrgv
      961 evpnfpaaat rrppvqsppa cvwavvqccp fntnrlvtcf esmgcpgvnw dpnpcrgsit
     1021 kaarqqitkf yaeseeenrg l
//
LOCUS       XP_034184993            1041 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184993
VERSION     XP_034184993.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329102.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184993.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1041
                     /product="uncharacterized protein LOC117606543 isoform X1"
                     /calculated_mol_wt=122271
     Region          <317..584
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <777..915
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1041
                     /gene="LOC117606543"
                     /coded_by="XM_034329102.2:219..3344"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mspplplslg mpeegempae aflqwrmqpg vtvvlatvwl yanvvgtasl nssrfsldqy
       61 splapqhely qrvpigsrrs sststasplq sfktnyldkd slhskskgns ggddddfspl
      121 vedkqrrkkf teqkidgggt kdseqpslal seledpfiad dtfqdqgpgs meiykldllr
      181 lyplnhvplt ryllkgkgkt pkktsqadwk akttdyksts pkmfykqsns fddlstevpp
      241 nsdpfsiggv pelqvgcegl aatkykqkrs idsggrdkap iaamdtwpgv tpisldmydf
      301 ssveeeedem delvrlkkle tttkgikqnr gdmeatrysd sddkgrpekl pvrgdlgksn
      361 gqnstggdqk tagegsnsdi akreafeeee aksadslvpa hqrrrilwld dsvadeqlrt
      421 egrskrqiwg fgedegvvss delqtenqrr rqdyerrrke eerrrleear rrghvenrtn
      481 severikqey erslqertrq eeerqrrlqq earrtssrwq speehrerpq elvasprrye
      541 syphtstyde errhqeaeam rrrdweterr rqqeldrrhl eqrrrewmlk rrqeeederr
      601 rqqarnepsn llyamraasn esgpyyvdpe rrfreqqere reeklkeymq rnrpinpadr
      661 qreeeirrrq deerrrieee rklqeyirrn qpvhvtsgng ssvdqrkkmd dwsraynwsr
      721 ypgqgngrrn hgpsaptnid prsfveerrr keasrrteed rirererlee eqlrkealrr
      781 eeearrlqsr ryeeqrkrle aarrdadrrr kelmeeqarr sqagrtrsen trhgyddrar
      841 leelrrrqdt slipanlvln esrrtmeqdr qrqeqdrqry eqerwrleae rrrqedsrak
      901 qgreheqrrr qeearlnalp vsariivhpa qssssprlss rtgfdgdidf pgvnpyrrgv
      961 evpnfpaaat rrppvqsppa cvwavvqccp fntnrlvtcf esmgcpgvnw dpnpcrgsit
     1021 kaarqqitkf yaeseeenrg l
//
LOCUS       XP_034184994            1017 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184994
VERSION     XP_034184994.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329103.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184994.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1017
                     /product="uncharacterized protein LOC117606543 isoform X2"
                     /calculated_mol_wt=119662
     Region          <293..560
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <753..891
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1017
                     /gene="LOC117606543"
                     /coded_by="XM_034329103.2:217..3270"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mrmqpgvtvv latvwlyanv vgtaslnssr fsldqyspla pqhelyqrvp igsrrsssts
       61 tasplqsfkt nyldkdslhs kskgnsggdd ddfsplvedk qrrkkfteqk idgggtkdse
      121 qpslalsele dpfiaddtfq dqgpgsmeiy kldllrlypl nhvpltryll kgkgktpkkt
      181 sqadwkaktt dykstspkmf ykqsnsfddl stevppnsdp fsiggvpelq vgceglaatk
      241 ykqkrsidsg grdkapiaam dtwpgvtpis ldmydfssve eeedemdelv rlkkletttk
      301 gikqnrgdme atrysdsddk grpeklpvrg dlgksngqns tggdqktage gsnsdiakre
      361 afeeeeaksa dslvpahqrr rilwlddsva deqlrtegrs krqiwgfged egvvssdelq
      421 tenqrrrqdy errrkeeerr rleearrrgh venrtnseve rikqeyersl qertrqeeer
      481 qrrlqqearr tssrwqspee hrerpqelva sprryesyph tstydeerrh qeaeamrrrd
      541 weterrrqqe ldrrhleqrr rewmlkrrqe eederrrqqa rnepsnllya mraasnesgp
      601 yyvdperrfr eqqerereek lkeymqrnrp inpadrqree eirrrqdeer rrieeerklq
      661 eyirrnqpvh vtsgngssvd qrkkmddwsr aynwsrypgq gngrrnhgps aptnidprsf
      721 veerrrkeas rrteedrire rerleeeqlr kealrreeea rrlqsrryee qrkrleaarr
      781 dadrrrkelm eeqarrsqag rtrsentrhg yddrarleel rrrqdtslip anlvlnesrr
      841 tmeqdrqrqe qdrqryeqer wrleaerrrq edsrakqgre heqrrrqeea rlnalpvsar
      901 iivhpaqsss sprlssrtgf dgdidfpgvn pyrrgvevpn fpaaatrrpp vqsppacvwa
      961 vvqccpfntn rlvtcfesmg cpgvnwdpnp crgsitkaar qqitkfyaes eeenrgl
//
LOCUS       XP_034184995            1015 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184995
VERSION     XP_034184995.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329104.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184995.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1015
                     /product="uncharacterized protein LOC117606543 isoform X3"
                     /calculated_mol_wt=119375
     Region          <291..558
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <751..889
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1015
                     /gene="LOC117606543"
                     /coded_by="XM_034329104.2:271..3318"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mqpgvtvvla tvwlyanvvg taslnssrfs ldqysplapq helyqrvpig srrssststa
       61 splqsfktny ldkdslhsks kgnsggdddd fsplvedkqr rkkfteqkid gggtkdseqp
      121 slalseledp fiaddtfqdq gpgsmeiykl dllrlyplnh vpltryllkg kgktpkktsq
      181 adwkakttdy kstspkmfyk qsnsfddlst evppnsdpfs iggvpelqvg ceglaatkyk
      241 qkrsidsggr dkapiaamdt wpgvtpisld mydfssveee edemdelvrl kkletttkgi
      301 kqnrgdmeat rysdsddkgr peklpvrgdl gksngqnstg gdqktagegs nsdiakreaf
      361 eeeeaksads lvpahqrrri lwlddsvade qlrtegrskr qiwgfgedeg vvssdelqte
      421 nqrrrqdyer rrkeeerrrl eearrrghve nrtnseveri kqeyerslqe rtrqeeerqr
      481 rlqqearrts srwqspeehr erpqelvasp rryesyphts tydeerrhqe aeamrrrdwe
      541 terrrqqeld rrhleqrrre wmlkrrqeee derrrqqarn epsnllyamr aasnesgpyy
      601 vdperrfreq qerereeklk eymqrnrpin padrqreeei rrrqdeerrr ieeerklqey
      661 irrnqpvhvt sgngssvdqr kkmddwsray nwsrypgqgn grrnhgpsap tnidprsfve
      721 errrkeasrr teedrirere rleeeqlrke alrreeearr lqsrryeeqr krleaarrda
      781 drrrkelmee qarrsqagrt rsentrhgyd drarleelrr rqdtslipan lvlnesrrtm
      841 eqdrqrqeqd rqryeqerwr leaerrrqed srakqgrehe qrrrqeearl nalpvsarii
      901 vhpaqssssp rlssrtgfdg didfpgvnpy rrgvevpnfp aaatrrppvq sppacvwavv
      961 qccpfntnrl vtcfesmgcp gvnwdpnpcr gsitkaarqq itkfyaesee enrgl
//
LOCUS       XP_034184997            1015 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606543 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184997
VERSION     XP_034184997.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329106.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184997.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1015
                     /product="uncharacterized protein LOC117606543 isoform X3"
                     /calculated_mol_wt=119375
     Region          <291..558
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <751..889
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1015
                     /gene="LOC117606543"
                     /coded_by="XM_034329106.2:175..3222"
                     /db_xref="GeneID:117606543"
ORIGIN      
        1 mqpgvtvvla tvwlyanvvg taslnssrfs ldqysplapq helyqrvpig srrssststa
       61 splqsfktny ldkdslhsks kgnsggdddd fsplvedkqr rkkfteqkid gggtkdseqp
      121 slalseledp fiaddtfqdq gpgsmeiykl dllrlyplnh vpltryllkg kgktpkktsq
      181 adwkakttdy kstspkmfyk qsnsfddlst evppnsdpfs iggvpelqvg ceglaatkyk
      241 qkrsidsggr dkapiaamdt wpgvtpisld mydfssveee edemdelvrl kkletttkgi
      301 kqnrgdmeat rysdsddkgr peklpvrgdl gksngqnstg gdqktagegs nsdiakreaf
      361 eeeeaksads lvpahqrrri lwlddsvade qlrtegrskr qiwgfgedeg vvssdelqte
      421 nqrrrqdyer rrkeeerrrl eearrrghve nrtnseveri kqeyerslqe rtrqeeerqr
      481 rlqqearrts srwqspeehr erpqelvasp rryesyphts tydeerrhqe aeamrrrdwe
      541 terrrqqeld rrhleqrrre wmlkrrqeee derrrqqarn epsnllyamr aasnesgpyy
      601 vdperrfreq qerereeklk eymqrnrpin padrqreeei rrrqdeerrr ieeerklqey
      661 irrnqpvhvt sgngssvdqr kkmddwsray nwsrypgqgn grrnhgpsap tnidprsfve
      721 errrkeasrr teedrirere rleeeqlrke alrreeearr lqsrryeeqr krleaarrda
      781 drrrkelmee qarrsqagrt rsentrhgyd drarleelrr rqdtslipan lvlnesrrtm
      841 eqdrqrqeqd rqryeqerwr leaerrrqed srakqgrehe qrrrqeearl nalpvsarii
      901 vhpaqssssp rlssrtgfdg didfpgvnpy rrgvevpnfp aaatrrppvq sppacvwavv
      961 qccpfntnrl vtcfesmgcp gvnwdpnpcr gsitkaarqq itkfyaesee enrgl
//
LOCUS       XP_034184998             942 aa            linear   INV 03-SEP-2025
DEFINITION  lysine ketoglutarate reductase/saccharopine dehydrogenase [Osmia
            lignaria lignaria].
ACCESSION   XP_034184998
VERSION     XP_034184998.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329107.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..942
                     /product="lysine ketoglutarate reductase/saccharopine
                     dehydrogenase"
                     /calculated_mol_wt=105037
     Region          37..937
                     /region_name="PLN02819"
                     /note="lysine-ketoglutarate reductase/saccharopine
                     dehydrogenase"
                     /db_xref="CDD:215439"
     CDS             1..942
                     /gene="LKRSDH"
                     /coded_by="XM_034329107.2:151..2979"
                     /db_xref="GeneID:117606544"
ORIGIN      
        1 mkilqlcksq snktcsflmt qttmssvryl ntlkgkiiai rredqsvwer raplapanvr
       61 rliragvkvi vqpsnrrayp ahsyqaagal lqedissasv ifgvkqvpvd qlisnktycf
      121 fshtikaqes nmplldaile knirlldyek ltddngqrvv afgkyagvag mvnilhglgl
      181 rllalghhtp fmhigpahny rdssmarqsi rdagyeialg ampksigplt fiftgsgnvs
      241 qggqevfqel pheyvppeml rkvaehgdpt kiygcevrrr hhlerkeggg fdpdeydkyp
      301 dryistfskk iasyasviin giywavdspk lltipdakyl lrpaytpwlp tsvgapalph
      361 rmlaicdisa dpggsiefmn ecttidtpfc lydadrnkdt ksfkgpgvlv csidnmptql
      421 pkestdffgn llypyaldii ksdarvplee hnftpavhda iiasngrlts nfeyiqelrl
      481 lnqrskhkad nsevqnkkvv vlgagyvsap lveylhrdsk ihlmvasqlk eeadvlanky
      541 pgvesvllnv lerpdtlkdi vetadvvvsl lpyslhhvig dvcikakthl vtasytnedv
      601 kalhdqavaa dvtilnevgl dpgidhllal ecfdnvrqag gkiesfvswc gglpapecss
      661 nplrykfsws prgvllntma pakfyhdkqv veiqpggdlm savqdldflp gfalegfpnr
      721 dstiykdlyg isnantmlrg tlrfkgfcnt iralqylglt dpkphpclhp ngpditwrvl
      781 icnllgland nifyenlkrk laemlnsees vqaieelgll eedlvlklnt pldtlthyls
      841 kklcfepner dlvilrhdig ilwpdnrrev rginlvvygd vkgysamart vgyptaiavk
      901 mildgeiqqr gvilpftpdi yrpmlnrlkt egieffetsk wl
//
LOCUS       XP_034185001             432 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606547 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185001
VERSION     XP_034185001.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329110.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185001.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..432
                     /product="uncharacterized protein LOC117606547 isoform X1"
                     /calculated_mol_wt=49354
     CDS             1..432
                     /gene="LOC117606547"
                     /coded_by="XM_034329110.2:51..1349"
                     /db_xref="GeneID:117606547"
ORIGIN      
        1 mevsktrkky khytclcvsv mgqrrfrism galipspeag dasvmipkmk kctaisddts
       61 ksfvstsern iinrstlmsa snydcindak naayehmvll edgvldemst stihdqcqfp
      121 yyrssknrgr fpdavlvkdv gvscmlsdrn astvhvpetk tlvkqlkkey ndisnitnki
      181 tmytkdilrh lknknkrkhr llqhlisskh viasqyidnt gnlclklrly snaetqctpr
      241 nikdvdnvfr dkcvtkkqrc khsnsrksck nqtacqestr sklekdiqte ilkenveist
      301 tnssapstss iynyltmrkr ktpsgkfnpv stklndihkh wnhkncgsnk dhkehlkrce
      361 pviftnwkei skskqskika ssvisdesee mtntkkptlq ksllnydmin nvpntmfhti
      421 niriveqstk hv
//
LOCUS       XP_034185002             388 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606547 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185002
VERSION     XP_034185002.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329111.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185002.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..388
                     /product="uncharacterized protein LOC117606547 isoform X2"
                     /calculated_mol_wt=44439
     CDS             1..388
                     /gene="LOC117606547"
                     /coded_by="XM_034329111.2:144..1310"
                     /db_xref="GeneID:117606547"
ORIGIN      
        1 mipkmkkcta isddtsksfv stserniinr stlmsasnyd cindaknaay ehmvlledgv
       61 ldemststih dqcqfpyyrs sknrgrfpda vlvkdvgvsc mlsdrnastv hvpetktlvk
      121 qlkkeyndis nitnkitmyt kdilrhlknk nkrkhrllqh lisskhvias qyidntgnlc
      181 lklrlysnae tqctprnikd vdnvfrdkcv tkkqrckhsn srkscknqta cqestrskle
      241 kdiqteilke nveisttnss apstssiyny ltmrkrktps gkfnpvstkl ndihkhwnhk
      301 ncgsnkdhke hlkrcepvif tnwkeisksk qskikassvi sdeseemtnt kkptlqksll
      361 nydminnvpn tmfhtiniri veqstkhv
//
LOCUS       XP_034185004             355 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606547 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185004
VERSION     XP_034185004.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329113.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185004.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="uncharacterized protein LOC117606547 isoform X3"
                     /calculated_mol_wt=40754
     CDS             1..355
                     /gene="LOC117606547"
                     /coded_by="XM_034329113.2:154..1221"
                     /db_xref="GeneID:117606547"
ORIGIN      
        1 msasnydcin daknaayehm vlledgvlde mststihdqc qfpyyrsskn rgrfpdavlv
       61 kdvgvscmls drnastvhvp etktlvkqlk keyndisnit nkitmytkdi lrhlknknkr
      121 khrllqhlis skhviasqyi dntgnlclkl rlysnaetqc tprnikdvdn vfrdkcvtkk
      181 qrckhsnsrk scknqtacqe strsklekdi qteilkenve isttnssaps tssiynyltm
      241 rkrktpsgkf npvstklndi hkhwnhkncg snkdhkehlk rcepviftnw keiskskqsk
      301 ikassvisde seemtntkkp tlqksllnyd minnvpntmf htiniriveq stkhv
//
LOCUS       XP_034185010             293 aa            linear   INV 03-SEP-2025
DEFINITION  non-homologous end-joining factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185010
VERSION     XP_034185010.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329119.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="non-homologous end-joining factor 1"
                     /calculated_mol_wt=33414
     Region          12..107
                     /region_name="HD_XLF_N"
                     /note="N-terminal head domain found in XRCC4-like factor
                     and similar proteins; cd22285"
                     /db_xref="CDD:409002"
     Site            40..41
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:409002"
     CDS             1..293
                     /gene="LOC117606553"
                     /coded_by="XM_034329119.2:110..991"
                     /db_xref="GeneID:117606553"
ORIGIN      
        1 mgfpqsnkgl vwnklkinde vymisatqkd dtieilltnf iqiwiekftd kisldrcrel
       61 npllnvealn ckeiilniln dipkhiveas peqiklrvkl eggfmkfaln likgtpedfw
      121 efvtkplcis sveiirqhkf lldlvkrkde eiaeykaega elirknietk pfmeeqlrtv
      181 issantddyt nmfqtitnfy nahlqkhnes svkssddkvk eeqnissqdg akpsegvise
      241 gstpiktkts pskskkqkkn tdndeeiink trnanmvyrp ikklkkgvpg sil
//
LOCUS       XP_034185011             293 aa            linear   INV 03-SEP-2025
DEFINITION  non-homologous end-joining factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185011
VERSION     XP_034185011.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329120.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="non-homologous end-joining factor 1"
                     /calculated_mol_wt=33414
     Region          12..107
                     /region_name="HD_XLF_N"
                     /note="N-terminal head domain found in XRCC4-like factor
                     and similar proteins; cd22285"
                     /db_xref="CDD:409002"
     Site            40..41
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:409002"
     CDS             1..293
                     /gene="LOC117606553"
                     /coded_by="XM_034329120.2:110..991"
                     /db_xref="GeneID:117606553"
ORIGIN      
        1 mgfpqsnkgl vwnklkinde vymisatqkd dtieilltnf iqiwiekftd kisldrcrel
       61 npllnvealn ckeiilniln dipkhiveas peqiklrvkl eggfmkfaln likgtpedfw
      121 efvtkplcis sveiirqhkf lldlvkrkde eiaeykaega elirknietk pfmeeqlrtv
      181 issantddyt nmfqtitnfy nahlqkhnes svkssddkvk eeqnissqdg akpsegvise
      241 gstpiktkts pskskkqkkn tdndeeiink trnanmvyrp ikklkkgvpg sil
//
LOCUS       XP_034185012             293 aa            linear   INV 03-SEP-2025
DEFINITION  non-homologous end-joining factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185012
VERSION     XP_034185012.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329121.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="non-homologous end-joining factor 1"
                     /calculated_mol_wt=33414
     Region          12..107
                     /region_name="HD_XLF_N"
                     /note="N-terminal head domain found in XRCC4-like factor
                     and similar proteins; cd22285"
                     /db_xref="CDD:409002"
     Site            40..41
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:409002"
     CDS             1..293
                     /gene="LOC117606553"
                     /coded_by="XM_034329121.2:110..991"
                     /db_xref="GeneID:117606553"
ORIGIN      
        1 mgfpqsnkgl vwnklkinde vymisatqkd dtieilltnf iqiwiekftd kisldrcrel
       61 npllnvealn ckeiilniln dipkhiveas peqiklrvkl eggfmkfaln likgtpedfw
      121 efvtkplcis sveiirqhkf lldlvkrkde eiaeykaega elirknietk pfmeeqlrtv
      181 issantddyt nmfqtitnfy nahlqkhnes svkssddkvk eeqnissqdg akpsegvise
      241 gstpiktkts pskskkqkkn tdndeeiink trnanmvyrp ikklkkgvpg sil
//
LOCUS       XP_034185013             257 aa            linear   INV 03-SEP-2025
DEFINITION  dnaJ homolog subfamily C member 8 [Osmia lignaria lignaria].
ACCESSION   XP_034185013
VERSION     XP_034185013.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..257
                     /product="dnaJ homolog subfamily C member 8"
                     /calculated_mol_wt=29944
     Region          57..114
                     /region_name="DnaJ"
                     /note="DnaJ molecular chaperone homology domain;
                     smart00271"
                     /db_xref="CDD:197617"
     Site            order(85..87,97,100..101,104..105)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..257
                     /gene="LOC117606554"
                     /coded_by="XM_034329122.2:32..805"
                     /db_xref="GeneID:117606554"
ORIGIN      
        1 maatnssips dnptkkedef nefytevkei ekrdsvltpk qqidrllrpg ssyfnlnpfe
       61 vlqidpstsi deikkkyrrm silvhpdknq ddaeraqqaf eivnkawktl eneetrakcm
      121 dvieeakart dhmiaekkkk lkkegktdsg aileeetdeg yrhavwvmtm klfadmerrr
      181 relatrdaeq rkrkreeels aeaqaalere wqknfeesrq srvdswkafq sgssatkqkk
      241 akklkafrpp ktkaesr
//
LOCUS       XP_034185014             248 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich melanocyte differentiation-associated protein [Osmia
            lignaria lignaria].
ACCESSION   XP_034185014
VERSION     XP_034185014.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329123.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="leucine-rich melanocyte
                     differentiation-associated protein"
                     /calculated_mol_wt=27819
     Region          <44..>105
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          44..64
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          65..86
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          <70..188
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          87..101
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          114..131
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..248
                     /gene="LOC117606555"
                     /coded_by="XM_034329123.2:79..825"
                     /db_xref="GeneID:117606555"
ORIGIN      
        1 madrgqqdfn rsaltvrggr awytgqraek ipgglvgvvg edctsldlsy nelssittvk
       61 hfqslqelil dnnklrdlkt lpciptlttl slnnnkitdi dsalkriqec canvvyvsll
      121 gnpgcpdqlt npsstdeedy dryrlyaiyv lpptlrflds rpvtrqekid aerrgkysrt
      181 vklvpelsst ivpnpveeid diffnihytp lptsirnpld hkgafgkcky rysgknsegn
      241 rfisnndl
//
LOCUS       XP_034185015             244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606556 [Osmia lignaria lignaria].
ACCESSION   XP_034185015
VERSION     XP_034185015.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329124.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..244
                     /product="uncharacterized protein LOC117606556"
                     /calculated_mol_wt=28303
     CDS             1..244
                     /gene="LOC117606556"
                     /coded_by="XM_034329124.2:401..1135"
                     /db_xref="GeneID:117606556"
ORIGIN      
        1 mgkgdkrgia qrsdastnlm gkftqsvrri vqdvkdegts sgqtkeevie tnerlrivri
       61 rldgsyeiak ralvelmcky tdskqvrnvf qrynllklmi kdvikletqy wtlvdipkqe
      121 kqetvpafvl racsimekth ksgegvktsa rlaeeaetkr erierlesmt taqieaentq
      181 mtndlyrllk kytglrnlir dlkeeynssk vypifprypi lkdmikdimh npdymevche
      241 vdqa
//
LOCUS       XP_034195133             223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611287 [Osmia lignaria lignaria].
ACCESSION   XP_034195133
VERSION     XP_034195133.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339242.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195133.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="uncharacterized protein LOC117611287"
                     /calculated_mol_wt=24913
     Region          13..101
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(27,29,31,51..53,90)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133158"
     Site            27..29
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133158"
     Site            27
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133158"
     Site            order(51..54,58..61)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133158"
     CDS             1..223
                     /gene="LOC117611287"
                     /coded_by="XM_034339242.2:1..672"
                     /db_xref="GeneID:117611287"
ORIGIN      
        1 mtaadglpnn crlvvkdcls nlrflidiga dnsalphrsa krestpsdfk iyaangtviq
       61 tygttrlvln lglrrpflwe fivaniqqpi igadflrkhg llvdlrnrkl idgltklhtv
      121 eqvstipqlp lttidnnnpy qdllrqfihv trtiqchtep khkvrhhiit kdapcaerar
      181 rlptekfkaa qaeinhliaq gicilssssw vsplhmvpkk ngg
//
LOCUS       XP_076545693             106 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305543 [Osmia lignaria lignaria].
ACCESSION   XP_076545693
VERSION     XP_076545693.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689578.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="uncharacterized protein LOC143305543"
                     /calculated_mol_wt=11862
     CDS             1..106
                     /gene="LOC143305543"
                     /coded_by="XM_076689578.1:81..401"
                     /db_xref="GeneID:143305543"
ORIGIN      
        1 maedkmdvdk eveekkqgve tkeeiekkee vekqeegtkk eevpkteeek lsisqikkmv
       61 kkliyggrhg ghrggrggrw ggrrggrggr rggrrgggrq vifnif
//
LOCUS       XP_076545694             469 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305544 [Osmia lignaria lignaria].
ACCESSION   XP_076545694
VERSION     XP_076545694.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689579.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="uncharacterized protein LOC143305544"
                     /calculated_mol_wt=52055
     Region          1..202
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(7,9,34,88,124,126,199)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(7,34,88,124,126,173,198..199)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(34,198)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..469
                     /gene="LOC143305544"
                     /coded_by="XM_076689579.1:1..1410"
                     /db_xref="GeneID:143305544"
ORIGIN      
        1 mkilqaninh crlahdmllv satekiadlv vvaepytipd nwfsdttgra aifitntgla
       61 kckqttivgc gigyvgisfn glgiisvytt pnanpqevev qfsalealvr dlqsshskil
      121 mlgdfnaksp lwgadrwcgr gkvlfqlats lglhptiavg gatcergggs vvdimlgndl
      181 alsphissev sdeftgsdhr yvwhsfresd svyavdhdlf dlgkgkidee rllaalikky
      241 gdeslerrgr tgtthevdkf iedlerlvnr yttyrratlr ahkpaywwnd avavkrkevn
      301 karrkytrar aknvtadtea ayaiykaakk alnrevnkak ktnwrellse idkdiwgrpy
      361 ktvmkklkgk ttkpdvmcpe eveavveklf ilkppdgqlp vnkgleinsv etagvgylhd
      421 ssylpavrht ngqhlsvsph ihrhtaisdv htapnviafh sndtykhla
//
LOCUS       XP_076545695             889 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305545 [Osmia lignaria lignaria].
ACCESSION   XP_076545695
VERSION     XP_076545695.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689580.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..889
                     /product="uncharacterized protein LOC143305545"
                     /calculated_mol_wt=100597
     Region          78..>118
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          <221..299
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          319..393
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(322,324,326,349..351,391)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            322..324
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            322
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(349..352,356..359)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          <479..584
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            506
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          678..795
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(682..685,718..720,723,748,781,793)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     CDS             1..889
                     /gene="LOC143305545"
                     /coded_by="XM_076689580.1:1..2670"
                     /db_xref="GeneID:143305545"
ORIGIN      
        1 mpsepgtsvp psfdelfrrf metvasgavs tvtsatsnka tadaipefsg tdlgedanqw
       61 cdivekitdk lpisqrlsla thalhgaakk wykgwegnpr twttfredlc svfvsedrlc
      121 erlscavnyn sdsaisysey vrnklkyygq tqisfkphel islvighvtd psvrqslmna
      181 rytttadlls gisnfvkvpr keknsdskga vykrrdqilr rrcyqcnetg hlqhdcprkr
      241 kaeslpkdpv krtnvptctf cskrgheese cwlkrrtpre dkgakktnsr hgeleanacf
      301 rldhqltpii lqdmvvrscl vdngatcslv keqvakrahc kidpyitivr glcdsavttv
      361 gqttaviqte evtaeldllv vsedtmtydv iigknivtqg iqiltnsege tklqrcslvp
      421 qgiqedstpq cftcdvgdvg vrgeveklld rynhmitsgn kvrcvstsel kiklkedkis
      481 iaedsiekta fvtpdghyef lrmpfglana pavfqraint algtlrnkia lvyiddvlip
      541 srtieeglky leivlqaldt agfsiniekc kffqktieyl grevsaegir pgkdkvrall
      601 espvpcnikq vrqfvglasy frkfvpefav rtacitkltk kneqwhwaee qekarhyile
      661 klssnpllvi fnperetelh tdasaigyga ilfqkvdgds rvvayfsrrt tpeesryhsy
      721 eletlaivna lrhfrvyllg ikfkiitdcn aikatstkkd llprvarwwi flqdfnfeie
      781 yrkgkyvehy lsrnppkdyr vnticegswl evaqrkdeet ksiierietn dtkttgfspy
      841 rllfgverst atadpaetel pdtndpidvi kdrelaserl tenaqkqnt
//
LOCUS       XP_076545696             175 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305546 [Osmia lignaria lignaria].
ACCESSION   XP_076545696
VERSION     XP_076545696.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689581.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 59% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="uncharacterized protein LOC143305546"
                     /calculated_mol_wt=19922
     Region          109..>152
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..175
                     /gene="LOC143305546"
                     /coded_by="XM_076689581.1:1..528"
                     /db_xref="GeneID:143305546"
ORIGIN      
        1 mssedfefli ngigpkikkk dtrfrkvvtv kerlaitlrf latgdsytsl qylfkvskql
       61 istivpevcq alveilkehi kmpstpndwl aisqkfeelw nfphcvgamd gkhvvlqapa
      121 hsgsdfynyk sqfsivlfal vdadynflyv dalltqikme yslkvigdkk eiqnh
//
LOCUS       XP_076545697             540 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305547 [Osmia lignaria lignaria].
ACCESSION   XP_076545697
VERSION     XP_076545697.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689582.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="uncharacterized protein LOC143305547"
                     /calculated_mol_wt=61253
     Region          248..414
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(303..308,337..338,363,365..366,410..411)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(303..308,337,365)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            338
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     CDS             1..540
                     /gene="LOC143305547"
                     /coded_by="XM_076689582.1:1..1623"
                     /db_xref="GeneID:143305547"
ORIGIN      
        1 matatmvpnt fviepfcpts ttwsrwlmrl egafriftik nearvpyllh yvgpasfdiv
       61 cnqlapqdpf eatyitltkt lgdfyepapl eivenykfhq rkqkegesiq eflaalqklc
      121 lhckfgeyvk talrnqlvfg liskkiqarl lelpdltlek atqvatamei sdkgtqqlqg
      181 etseyqdits ptvakiknvq akltlkenak pifckarpvp frlrtqieee llrletegil
      241 ikvdtaewai pivpvlkkng qicicgdfsv tlnaqlivdd hllptadelf asmaggvifs
      301 kidllqaylq mevrpedrhl ltlnthkgly rctrlmygia svpaiwqrti esilgdipgv
      361 avflddiria gssvedhlrt lelvftrlqk ynirinpdks effarqinyc gyvidknglh
      421 kaldkikaie gmrgpqnvte qtllqyrnak hnttgkspae lmfnrklrtr ldlllkianl
      481 arlhykvkld dgriwkrhvn qmreigentl pkptdyssvv qkesdfstvg dtptrvetan
//
LOCUS       XP_076545698            1748 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611059 [Osmia lignaria lignaria].
ACCESSION   XP_076545698
VERSION     XP_076545698.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689583.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1748
                     /product="uncharacterized protein LOC117611059"
                     /calculated_mol_wt=196939
     Region          176..315
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          <499..619
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          755..971
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(818..823,858..859,894,896..897,967..968)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(818..823,858,896)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            859
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          988..1149
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          1368..1422
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1468..1559
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          1647..1739
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1748
                     /gene="LOC117611059"
                     /coded_by="XM_076689583.1:1..5247"
                     /db_xref="GeneID:117611059"
ORIGIN      
        1 mekfqanste tleedfivek yvsynhpevs apverpqtta evslqnlleh takrilllqt
       61 evidsmisks sddiiqsemi csygldgssg qwtykqqfkt nievaaseed tranpyfssd
      121 vfgdteaafl aaldamtaal ptdsepasns cntstascnr etalsvklpr velpkfsgdy
      181 tewenfrdlf qslvgsndal pevqklhylk lsligeasll lknvstsaan yeaawklltd
      241 ryvnecalit ahlkmlfdsp pigsavlsdl kslrdrsraa ltalknmarp veywddvlvf
      301 hfvrkldkes hrewelrmge eelfpsladw eaflnvrirt leslassrge gysacknksa
      361 piktvkahln asetkcvacg adhalfrcdv frgqsveqrl slarehhrcf nclqkghlpa
      421 acpskraysi ckgkhhtllh serkgsktss vskgrestsh adlesgqhsa qptyvadnnm
      481 edttmstsgr kqrsfvrmlr anrqhvqasv sgigagtsat vrasanvtie pchgkgpvip
      541 vralilkrlt nyrsnlidas skwihlrgle wadpltnnka pvdliigadn ygailldslq
      601 kglpsepvaq ktifgwvltg parstesham qlhvhygdln teinntlrkf weveapppvm
      661 flseedkqce dhfrrthsrk sdgkyvarlp fkseppidig askgiavasf yrlekrlakd
      721 aeqatayhsf leeylrlghm reflesdppl eqkvylshhp virqdssttk lgvvfnasmp
      781 tsngstlndh llvgpklqad lavtilrwrm yqfvfaadia kmfrqiwidh rdiayqlivw
      841 rakpedqlkt yelltvtygt apapflanrv ikqlaldegt nfplaapiln ehlyvddalf
      901 gaddrvlarq trqqvtdalr rggfhlrkws sncpqlltei shadhelass rlfqdaeglk
      961 mlgirwlpks defrfqfkfd sastanptkr vvlsniarif dplgwistvv ivakifmqrl
     1021 wvakldwdes lpceltnqwr eysaelkdlp kvtlprwirm gnatiayeih gfadasqday
     1081 aaviylrtis iadevalhll maktkvaplk pltiprlelc acvlmtrlie qvqatlkldn
     1141 vpvydwtdst vtlawihrhp sqlktftahr vaeiqsrapn aswrhvptgc npadcasrgl
     1201 avklllhhpl wwhgpawlaq tpnewpefrp vvpreaeaei knqinalhve setewdlptr
     1261 tsswqkllri tayvmrfilr vktgkhdhqd tvvttnelva srkfwvqqvq arsfrneika
     1321 lkkseslaks splfrlnpfl dedgtirvgg rlrnsdlmer fpivlpvhrv teliiadcha
     1381 ntlhggiqlt lsvlrrtywv inarrlvksh iykcircvrw raatvrqqms dlptarvtpv
     1441 rpfahcgvdy agpfsalahr grgqkahkvy ialficlstr aihlelvrdy staaflaafn
     1501 rftarrglpt klysdngtnf qgankelraa fklvtadshl ralfaadhit wqfippssph
     1561 fggiweagvk svkthlrrml cdrtptceef ntllcqieac lnsrplaslk ddlescealt
     1621 pghflvggvf kvppapsvid itenrlsrwq wvqqltekfw kawtheylqs lqarskwcsr
     1681 atnlkigdlv llksstlppg kwdlgrvtec hpgkdglvrv vsiktarsey thpvvnlckp
     1741 pissldlv
//
LOCUS       XP_076545699             636 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305548 [Osmia lignaria lignaria].
ACCESSION   XP_076545699
VERSION     XP_076545699.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689584.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="uncharacterized protein LOC143305548"
                     /calculated_mol_wt=71575
     Region          <11..251
                     /region_name="YkfC"
                     /note="Retron-type reverse transcriptase [Mobilome:
                     prophages, transposons]; COG3344"
                     /db_xref="CDD:442573"
     Region          <61..173
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            65
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238827"
     Region          360..483
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(362..365,392..394,397,423,461,479)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     Region          <561..599
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..636
                     /gene="LOC143305548"
                     /coded_by="XM_076689584.1:1..1911"
                     /db_xref="GeneID:143305548"
ORIGIN      
        1 malelnctnt fdntphdaiq eaavrynikd svvrwihnml tnrtvtaslg eetvradvar
       61 gcpqggvlsp llwtlvvdel irilttegfa vqgyaddlti tvrgrhplel arrlqkaitl
      121 veswcrshsl sanpsktelv lftrrrrfyf kaphlfgtql qltdevkylg vtldkkltwk
      181 nhvnrqtqka istywacrrl fgptwglkpr mvrwiyqail epiityasiv wwtsmkrgah
      241 rrtieriyrl allgitgalp ttptmalgal ldikpphltv matawraair lhqaeewcsa
      301 sgghteilrd rgselilthg gdrcktvylf rreydviipe rekwledpsv ilnpgglvwf
      361 tdgsktgsgt aagiagdspk vemtyklghh vtvfqaevfa iwacakynle kdhsgkhiyi
      421 csdsiaalga ihkvevgskl vkdciltlrq lslnnrvkll wvpghagipg neranelarl
      481 gatssqpche ypigvstyal kslakdwlnq eftrlwnnan gmrhtralfe gpsqklgdtl
      541 ihldrarlrm lvglvtghwy trkhlarmrl teetlcprce eedetplhil lrcrelralr
      601 gsilgtlels slsisaglvp vllnfateaq lprysq
//
LOCUS       XP_076545700             115 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305549 [Osmia lignaria lignaria].
ACCESSION   XP_076545700
VERSION     XP_076545700.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689585.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="uncharacterized protein LOC143305549"
                     /calculated_mol_wt=13398
     CDS             1..115
                     /gene="LOC143305549"
                     /coded_by="XM_076689585.1:395..742"
                     /db_xref="GeneID:143305549"
ORIGIN      
        1 msdhemevdr neagkkgekv erreeekenk eaekerrkee ekqeekqqsa ssssssqmev
       61 drneagkkge kverreeeke nkeaekerrk eeekqeekqq sassssssqv lrraa
//
LOCUS       XP_034195030             109 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611203 [Osmia lignaria lignaria].
ACCESSION   XP_034195030
VERSION     XP_034195030.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339139.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195030.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 24% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..109
                     /product="uncharacterized protein LOC117611203"
                     /calculated_mol_wt=11876
     CDS             1..109
                     /gene="LOC117611203"
                     /coded_by="XM_034339139.2:475..804"
                     /db_xref="GeneID:117611203"
ORIGIN      
        1 msdhemevvn tdenkedien iqekkeeekq kedgkngqkd gknrqeekek plssskikkl
       61 lkkmvygggg grrgkwggrr ggrgwrgggg ggrgrgggtg grnvivnyy
//
LOCUS       XP_076545701             304 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305550 [Osmia lignaria lignaria].
ACCESSION   XP_076545701
VERSION     XP_076545701.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689586.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="uncharacterized protein LOC143305550"
                     /calculated_mol_wt=35436
     Region          <95..264
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..304
                     /gene="LOC143305550"
                     /coded_by="XM_076689586.1:1..915"
                     /db_xref="GeneID:143305550"
ORIGIN      
        1 mgfkwrktnn irkvliekps ivtwraryls dieyyrqqnr nivyidetwv dntlcfskcw
       61 qseeilgitr stssshrlii vhaggkdgfi kgtelifkpr tttgdyhgem nasnfekwin
      121 eklipnlptk svvvmdnapy hsvqinkpps tyskkqemil wlvtnnipht pemrkhqlle
      181 likknknpek mfkvdetlks hghsvlrlpp ymcelnpmel awskvkrkir erntsllssn
      241 elrmyaeeav nevtsadwhn fckhtekiea eywqkdglve evievidlhp egletdsdds
      301 tsed
//
LOCUS       XP_076545702            1410 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610838 [Osmia lignaria lignaria].
ACCESSION   XP_076545702
VERSION     XP_076545702.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689587.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1410
                     /product="uncharacterized protein LOC117610838"
                     /calculated_mol_wt=158428
     Region          <4..>59
                     /region_name="PRK10905"
                     /note="cell division protein DamX; Validated"
                     /db_xref="CDD:236792"
     Region          185..325
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          462..>560
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(479,481,483,506..508)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            479..481
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            479
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(506..509,513..516)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          690..906
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(755..760,794..795,830,832..833,902..903)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(755..760,794,832)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            795
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          921..1082
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <1049..1124
                     /region_name="RnhA"
                     /note="Ribonuclease HI [Replication, recombination and
                     repair]; COG0328"
                     /db_xref="CDD:440097"
     CDS             1..1410
                     /gene="LOC117610838"
                     /coded_by="XM_076689587.1:1..4233"
                     /db_xref="GeneID:117610838"
ORIGIN      
        1 msdkeekpak atpqtskpgs rtasptpvaa tlavptalpt eappptvekr atrssspagl
       61 smeltfkaks qmsrvemlrr imdkmpemld ettpkdiesi saqvdeihkl fqkehaylev
      121 twpasqashp ylkedyyele mgvvlevrrr lgkfrvafek lrerrgesta rrtpsklpel
      181 tlptftgrye awpafcelfa siiidnerls nverlhylrs clrseslrvi anlpstgesf
      241 piawanlkqr yenkriiiss lldklfstsp vqqrtaaayh nvaanlrdyn tslralaspe
      301 elwnctlvhv msrslvkttr eawetslgtt tefpkpekle eflttkaral ervelpaaaq
      361 ahaqakaktt gqrpaltttq aatiarasah aypcdccqed hfvisctkfr amtvperrrl
      421 vdklslcpqt qwvndpaqpp tpdqrscsla tqaeltllat anaqvitssa thnarllvds
      481 gselsfvsed lveqlglqrt qstisitgig gkkstqtrvl iqthilrtlt atlpssealk
      541 edwphlnrlk ladpdfykpr arvyssfnat ifqdsdaalr elltkfwiqe epptettsql
      601 tpeeqecenh flathgrdtt gryvariplk sntkvlgtsy starsclrrt lnklsrnpey
      661 reqyqrfmse yedlghmkka ssislndssi yylphhgvfk pdsettklrv vfngsspttt
      721 glsvddimht ganlllnitd vllwirhhkc ifatditkmy rqvrvheddw nlqrilwide
      781 esnevpytlt tvtygtkaap flavrallql aedegqryll avpsikhary vdnifggads
      841 ldslieiasq ltdlcnaggf plakwhsnea slletivpcn nsqgtsisld dcntktlglr
      901 wntvndsftf ttkshhnlnf tkrlvlsevv qifdplgfls pviirakvll qelwllnlkw
      961 ddplpshvtt rwiairedlt slarlsiprw fntynnstve lhgfsdasql amaaviyitv
     1021 fspftdlkvt slvcsktkva plkkltiprl eltaalifak llkyvqanlk lniaaihlwt
     1081 dsqvtltwik shasrwkdyv rnrvtqiqel tqtatwmhvp gtsnpadcas rgltttqleq
     1141 helwwrgppw llqsqeswpv qrnasddtcl qecrpgishv ltfisklrnt tastrlsntp
     1201 adlekakifw ikatqsayfs helkmmvnnq tlpsshafnr ltafiddqgv irvggrlsna
     1261 qltyedsalk smfvegtqdn qrlaklfvdd httwsfnppa aphmggkwea vvksvkyhlr
     1321 rtqsqhclsh llnrptshre pggisynkny nnsgsagqln isngcnqsls gstlpttstl
     1381 dlwcclqtsa shhasgllle csqftldqmd
//
LOCUS       XP_076545703             319 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein C21orf140 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_076545703
VERSION     XP_076545703.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689588.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..319
                     /product="uncharacterized protein C21orf140 homolog"
                     /calculated_mol_wt=36383
     Region          <1..232
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     Region          <93..256
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..319
                     /gene="LOC143305551"
                     /coded_by="XM_076689588.1:1..960"
                     /db_xref="GeneID:143305551"
ORIGIN      
        1 mgyrwkksts irkilvekpn ivawrdkylt aidsyrkqgr niiyldetwv dntlsfskcw
       61 qsneemgilk nnssshryii vhaggeegfv ngaalifkar sttgdyhgqm nalnfekwin
      121 ekllpniplh siivmdnapy htvqinkppt aygkksdmik wlgenniqha atmkkyqlve
      181 liqqnksldk tykvdelfkr hghnvlilpp ymcelnpiel awakvkriir enntstltai
      241 eltnltekai ndvstedwvg fckhvqkiek qyverdname simeeivdtr pmnatsctds
      301 sdsdtnssss sssssnvqd
//
LOCUS       XP_034195057            1076 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611229 [Osmia lignaria lignaria].
ACCESSION   XP_034195057
VERSION     XP_034195057.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339166.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195057.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1076
                     /product="uncharacterized protein LOC117611229"
                     /calculated_mol_wt=122032
     Region          136..347
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(200..205,240..241,276,278..279,343..344)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(200..205,240,278)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            241
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          364..524
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          745..795
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1021..>1076
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1076
                     /gene="LOC117611229"
                     /coded_by="XM_034339166.2:1..3231"
                     /db_xref="GeneID:117611229"
ORIGIN      
        1 mgpasddkpk aetaarvlhc hgedstsqll qrfweldeir erapmsqeee kcerhfldth
       61 ardpsgryvv rlpfvkdprt alksnrttal kllfncerrl ssdatlkeqy rnfmmeyltl
      121 qhmqgapeea dkgpayylph havrkrhdps aklrvvfnas fctatgqsln dclatgqklq
      181 adlwmvltrw rlfrvvfttd ivkmfrqirv hsedtkwqsi lwratpderv qdfrlttvty
      241 gtacapflal rvlaqlande ssrfprgasv vrrhtyvddi ltgaddvset lalkrevvai
      301 fkaggfelsk wasnipeiqe adssntrlfq ewsgistlgv nwnprddafs lrvaaaelvr
      361 ecttkrsils eiaglfdplg waapvlvvak ilmqdlwilg adwdqqlpen vctmwqrfrg
      421 slqqldtlki prwtfassep stqmelhgfc dasqrayaaa vylrvnnngs vttsllvakt
      481 kvapvktiti pklelcgatl lskllirvke ginmsgptia wtdssvtlhw irghasqwkp
      541 fvahrvsdiq heipadnwrh irspdnpadl atrgmspael vdsdiwwhgp kwlteqpefw
      601 patfpesneg ieaerrgatv hvvkekfgen flcrfsslsh llsviaqcfr faslsrgqpc
      661 ekgfvkaaer thaflavlrf sqhtsfleei eqlqrhgelr kasplspcrp flddqgllrl
      721 ggrlenaalp ynekhpylvs krcplarlli rdahyrtlhg gpqltrshlm rrvwivrgrs
      781 lvrseiaacv tcarfsgrra gqlmgqlpqe rtaaqraflt tgvdyagpik lrtspgrghk
      841 aykgyialfv clstraihle avsdltsasf laafrrfvgr rghcatlfsd ngtvfqgadr
      901 elrtmfrqst lfyqeaaasl aldgtswqfi ppyaphfggl weagvksvkh hlrrvigdst
      961 ltfeelttll cqieaclnsr plyalsedps elaaltpghf ligepttvpp epeepctalh
     1021 trwrlisqmr aqfwkrwrre ylqhlqqfpk wrryrenlei gslalirddl qppakw
//
LOCUS       XP_076545704             368 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305552 [Osmia lignaria lignaria].
ACCESSION   XP_076545704
VERSION     XP_076545704.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689589.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..368
                     /product="uncharacterized protein LOC143305552"
                     /calculated_mol_wt=42092
     CDS             1..368
                     /gene="LOC143305552"
                     /coded_by="XM_076689589.1:116..1222"
                     /db_xref="GeneID:143305552"
ORIGIN      
        1 mrmsldgaqp ikpaflptan trnantngir khfeqtsdyr revqlprmns nmemehrpkr
       61 mankparyqt tssdeeprkr lkvapgsive ditdlratle gvddevlsnn yrqhehtnty
      121 tpldtyspyt stytpsnini ysphtppnty rppptayapp tqsfhipdav esrpeprmqq
      181 gteptnlqir dlytkignle smvtkthsll ekvakqqqhl lhqhhgipsk paflpissla
      241 gmtefqnide dsyrevvnyl eyvggfnlke avtlsfketv sdclltsysw lgrednnlal
      301 cetrivhaih davlqnkhfp rpsrkdvaeh lkaalrtake rvrskrrgpr npqhetvdnf
      361 wtakeqsr
//
LOCUS       XP_076545705             328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611080 [Osmia lignaria lignaria].
ACCESSION   XP_076545705
VERSION     XP_076545705.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689590.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="uncharacterized protein LOC117611080"
                     /calculated_mol_wt=36843
     Region          <249..>303
                     /region_name="PDDEXK_nuclease-like"
                     /note="PDDEXK family nucleases; cl40440"
                     /db_xref="CDD:477358"
     CDS             1..328
                     /gene="LOC117611080"
                     /coded_by="XM_076689590.1:1..987"
                     /db_xref="GeneID:117611080"
ORIGIN      
        1 miaemrlvea slssresygd saigyvclrr elsictikgk icpehrvksk ryavtlvvne
       61 adetiqevnc edcavsqggc khalaflmwl hckseqrspt eveyywnkpi lgtvgttkkf
      121 veatelckee ipstvlpdns tflqnilkka eekqidsqls rhnfellere cyslslhhll
      181 iaflknggsa andfiqfats kmtpqliskv evetrqqses slwcvshsps sqttqfrfha
      241 akkfdsaltr rgknlektvi aeveklinkk lkkcgiilip qcpmlgaspd amgenfvvev
      301 kcpgsdktfs qyisnneity peferaek
//
LOCUS       XP_076545706             308 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611040 [Osmia lignaria lignaria].
ACCESSION   XP_076545706
VERSION     XP_076545706.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689591.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="uncharacterized protein LOC117611040"
                     /calculated_mol_wt=36546
     Region          <163..280
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..308
                     /gene="LOC117611040"
                     /coded_by="XM_076689591.1:175..1101"
                     /db_xref="GeneID:117611040"
ORIGIN      
        1 mnltflvfhe qcttnhynvq lykyqfskmk fyeflalttn rdellnylys knvirqtikc
       61 pqcgsilelk cleslcfhct nhyykqitnr krkrltcnfk isalhrtwfs kanlnigvac
      121 rlicyilmmn tprqrfleve lnissstavd wtnfcrevik ynrgrviqgq wifgaierhs
      181 kkffvvpias rksevllpli lkniapgtii ysdcwkayae inkdvykhyt vnhsanfvdp
      241 etgvhtqnie rlwreirgni plygrkechf nyylaefifk kkfdfserld affkimgimy
      301 plneslne
//
LOCUS       XP_034185197             552 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185197
VERSION     XP_034185197.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329306.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..552
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X1"
                     /calculated_mol_wt=59912
     Region          47..552
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..552
                     /gene="heph"
                     /coded_by="XM_034329306.2:52..1710"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mrgsdellsq sgavsngaam spqphhaadn nndakkvkld kshngkpsrv ihirnipnev
       61 segeivhlgv pfgrvtnvlv lkgknqafle madenaaatm vsyyasciaq lrgravyvqf
      121 snhrelktdq thannansnn qvalpgqnqv aqtqaetqgg pntvlrvive qmvypisldv
      181 lyqiftrfgk vlkivtftkn stfqaliqya dmlsaqtakl slegqniyns cctlridysk
      241 mqnlnvkynn dksrdytnps lptgdanlda aslalggell pqllmgagsq praripesia
      301 gapgvlptpf aamhglpspl agpyngvppa gglaglggfp lgaaglgvrv ptnaqtsavl
      361 lvsnlneemv tpdalftlfg vygdvqrvki lynkkdsali qmaephqahl althmdklrv
      421 fgkqikvmls khqtvqlpke gqpdagltkd ytnsplhrfk kpgsknyqni yppsstlhls
      481 nipttvteee irdaftkngf tvkafkffpk drkmaliqmp smddavaali kmhnyqlses
      541 nhlrvsfsks ni
//
LOCUS       XP_034185198             548 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185198
VERSION     XP_034185198.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329307.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..548
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X2"
                     /calculated_mol_wt=59511
     Region          47..548
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..548
                     /gene="heph"
                     /coded_by="XM_034329307.2:52..1698"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mrgsdellsq sgavsngaam spqphhaadn nndakkvkld kshngkpsrv ihirnipnev
       61 segeivhlgv pfgrvtnvlv lkgknqafle madenaaatm vsyyasciaq lrgravyvqf
      121 snhrelktdq thannansnn qvalpgqnqv aqtqaetqgg pntvlrvive qmvypisldv
      181 lyqiftrfgk vlkivtftkn stfqaliqya dmlsaqtakl slegqniyns cctlridysk
      241 mqnlnvkynn dksrdytnps lptgdanlda aslalggell pqllmgagsq praripgapg
      301 vlptpfaamh glpsplagpy ngvppaggla glggfplgaa glgvrvptna qtsavllvsn
      361 lneemvtpda lftlfgvygd vqrvkilynk kdsaliqmae phqahlalth mdklrvfgkq
      421 ikvmlskhqt vqlpkegqpd agltkdytns plhrfkkpgs knyqniypps stlhlsnipt
      481 tvteeeirda ftkngftvka fkffpkdrkm aliqmpsmdd avaalikmhn yqlsesnhlr
      541 vsfsksni
//
LOCUS       XP_034185200             597 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185200
VERSION     XP_034185200.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329309.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X3"
                     /calculated_mol_wt=64704
     Region          91..597
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..597
                     /gene="heph"
                     /coded_by="XM_034329309.2:892..2685"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mmmanlppvm lppltdygml tptlgikvsv pefqyargtg asltmrgsde llsqsgavsn
       61 gaamspqphh aadnnndakk vkldkshngk psrvihirni pnevsegeiv hlgvpfgrvt
      121 nvlvlkgknq aflemadena aatmvsyyas ciaqlrgrav yvqfsnhrel ktdqthanna
      181 nsnnqvalpg qnqvaqtqae tqggpntvlr viveqmvypi sldvlyqift rfgkvlkivt
      241 ftknstfqal iqyadmlsaq taklslegqn iynscctlri dyskmqnlnv kynndksrdy
      301 tnpslptgda nldaaslalg gellpqllmg agsqprarip vesiagapgv lptpfaamhg
      361 lpsplagpyn gvppagglag lggfplgaag lgvrvptnaq tsavllvsnl neemvtpdal
      421 ftlfgvygdv qrvkilynkk dsaliqmaep hqahlalthm dklrvfgkqi kvmlskhqtv
      481 qlpkegqpda gltkdytnsp lhrfkkpgsk nyqniyppss tlhlsniptt vteeeirdaf
      541 tkngftvkaf kffpkdrkma liqmpsmdda vaalikmhny qlsesnhlrv sfsksni
//
LOCUS       XP_034185201             579 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185201
VERSION     XP_034185201.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329310.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..579
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X4"
                     /calculated_mol_wt=62808
     Region          73..579
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..579
                     /gene="heph"
                     /coded_by="XM_034329310.2:888..2627"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mmmanlppvm lppltdygml tptlgikrgs dellsqsgav sngaamspqp hhaadnnnda
       61 kkvkldkshn gkpsrvihir nipnevsege ivhlgvpfgr vtnvlvlkgk nqaflemade
      121 naaatmvsyy asciaqlrgr avyvqfsnhr elktdqthan nansnnqval pgqnqvaqtq
      181 aetqggpntv lrviveqmvy pisldvlyqi ftrfgkvlki vtftknstfq aliqyadmls
      241 aqtaklsleg qniynscctl ridyskmqnl nvkynndksr dytnpslptg danldaasla
      301 lggellpqll mgagsqprar ipvesiagap gvlptpfaam hglpsplagp yngvppaggl
      361 aglggfplga aglgvrvptn aqtsavllvs nlneemvtpd alftlfgvyg dvqrvkilyn
      421 kkdsaliqma ephqahlalt hmdklrvfgk qikvmlskhq tvqlpkegqp dagltkdytn
      481 splhrfkkpg sknyqniypp sstlhlsnip ttvteeeird aftkngftvk afkffpkdrk
      541 maliqmpsmd davaalikmh nyqlsesnhl rvsfsksni
//
LOCUS       XP_034185202             571 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185202
VERSION     XP_034185202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..571
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X5"
                     /calculated_mol_wt=61907
     Region          65..571
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..571
                     /gene="heph"
                     /coded_by="XM_034329311.2:465..2180"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mvsvpefqya rgtgasltmr gsdellsqsg avsngaamsp qphhaadnnn dakkvkldks
       61 hngkpsrvih irnipnevse geivhlgvpf grvtnvlvlk gknqaflema denaaatmvs
      121 yyasciaqlr gravyvqfsn hrelktdqth annansnnqv alpgqnqvaq tqaetqggpn
      181 tvlrviveqm vypisldvly qiftrfgkvl kivtftknst fqaliqyadm lsaqtaklsl
      241 egqniynscc tlridyskmq nlnvkynndk srdytnpslp tgdanldaas lalggellpq
      301 llmgagsqpr aripvesiag apgvlptpfa amhglpspla gpyngvppag glaglggfpl
      361 gaaglgvrvp tnaqtsavll vsnlneemvt pdalftlfgv ygdvqrvkil ynkkdsaliq
      421 maephqahla lthmdklrvf gkqikvmlsk hqtvqlpkeg qpdagltkdy tnsplhrfkk
      481 pgsknyqniy ppsstlhlsn ipttvteeei rdaftkngft vkafkffpkd rkmaliqmps
      541 mddavaalik mhnyqlsesn hlrvsfsksn i
//
LOCUS       XP_034185203             563 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185203
VERSION     XP_034185203.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329312.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..563
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X6"
                     /calculated_mol_wt=61049
     Region          57..563
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..563
                     /gene="heph"
                     /coded_by="XM_034329312.2:728..2419"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mngdtlhsgq krgsdellsq sgavsngaam spqphhaadn nndakkvkld kshngkpsrv
       61 ihirnipnev segeivhlgv pfgrvtnvlv lkgknqafle madenaaatm vsyyasciaq
      121 lrgravyvqf snhrelktdq thannansnn qvalpgqnqv aqtqaetqgg pntvlrvive
      181 qmvypisldv lyqiftrfgk vlkivtftkn stfqaliqya dmlsaqtakl slegqniyns
      241 cctlridysk mqnlnvkynn dksrdytnps lptgdanlda aslalggell pqllmgagsq
      301 praripvesi agapgvlptp faamhglpsp lagpyngvpp agglaglggf plgaaglgvr
      361 vptnaqtsav llvsnlneem vtpdalftlf gvygdvqrvk ilynkkdsal iqmaephqah
      421 lalthmdklr vfgkqikvml skhqtvqlpk egqpdagltk dytnsplhrf kkpgsknyqn
      481 iyppsstlhl snipttvtee eirdaftkng ftvkafkffp kdrkmaliqm psmddavaal
      541 ikmhnyqlse snhlrvsfsk sni
//
LOCUS       XP_034185199             559 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185199
VERSION     XP_034185199.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329308.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185199.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..559
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X7"
                     /calculated_mol_wt=60712
     Region          53..559
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..559
                     /gene="heph"
                     /coded_by="XM_034329308.2:1..1680"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 meniesqrgs dellsqsgav sngaamspqp hhaadnnnda kkvkldkshn gkpsrvihir
       61 nipnevsege ivhlgvpfgr vtnvlvlkgk nqaflemade naaatmvsyy asciaqlrgr
      121 avyvqfsnhr elktdqthan nansnnqval pgqnqvaqtq aetqggpntv lrviveqmvy
      181 pisldvlyqi ftrfgkvlki vtftknstfq aliqyadmls aqtaklsleg qniynscctl
      241 ridyskmqnl nvkynndksr dytnpslptg danldaasla lggellpqll mgagsqprar
      301 ipvesiagap gvlptpfaam hglpsplagp yngvppaggl aglggfplga aglgvrvptn
      361 aqtsavllvs nlneemvtpd alftlfgvyg dvqrvkilyn kkdsaliqma ephqahlalt
      421 hmdklrvfgk qikvmlskhq tvqlpkegqp dagltkdytn splhrfkkpg sknyqniypp
      481 sstlhlsnip ttvteeeird aftkngftvk afkffpkdrk maliqmpsmd davaalikmh
      541 nyqlsesnhl rvsfsksni
//
LOCUS       XP_034185204             553 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185204
VERSION     XP_034185204.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329313.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X8"
                     /calculated_mol_wt=60011
     Region          47..553
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..553
                     /gene="heph"
                     /coded_by="XM_034329313.2:465..2126"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mrgsdellsq sgavsngaam spqphhaadn nndakkvkld kshngkpsrv ihirnipnev
       61 segeivhlgv pfgrvtnvlv lkgknqafle madenaaatm vsyyasciaq lrgravyvqf
      121 snhrelktdq thannansnn qvalpgqnqv aqtqaetqgg pntvlrvive qmvypisldv
      181 lyqiftrfgk vlkivtftkn stfqaliqya dmlsaqtakl slegqniyns cctlridysk
      241 mqnlnvkynn dksrdytnps lptgdanlda aslalggell pqllmgagsq praripvesi
      301 agapgvlptp faamhglpsp lagpyngvpp agglaglggf plgaaglgvr vptnaqtsav
      361 llvsnlneem vtpdalftlf gvygdvqrvk ilynkkdsal iqmaephqah lalthmdklr
      421 vfgkqikvml skhqtvqlpk egqpdagltk dytnsplhrf kkpgsknyqn iyppsstlhl
      481 snipttvtee eirdaftkng ftvkafkffp kdrkmaliqm psmddavaal ikmhnyqlse
      541 snhlrvsfsk sni
//
LOCUS       XP_076545707             553 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X9 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545707
VERSION     XP_076545707.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689592.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X9"
                     /calculated_mol_wt=60011
     Region          47..553
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..553
                     /gene="heph"
                     /coded_by="XM_076689592.1:787..2448"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mrgsdellsq sgavsngaam spqphhaadn nndakkvkld kshngkpsrv ihirnipnev
       61 segeivhlgv pfgrvtnvlv lkgknqafle madenaaatm vsyyasciaq lrgravyvqf
      121 snhrelktdq thannansnn qvalpgqnqv aqtqaetqgg pntvlrvive qmvypisldv
      181 lyqiftrfgk vlkivtftkn stfqaliqya dmlsaqtakl slegqniyns cctlridysk
      241 mqnlnvkynn dksrdytnps lptgdanlda aslalggell pqllmgagsq praripvesi
      301 agapgvlptp faamhglpsp lagpyngvpp agglaglggf plgaaglgvr vptnaqtsav
      361 llvsnlneem vtpdalftlf gvygdvqrvk ilynkkdsal iqmaephqah lalthmdklr
      421 vfgkqikvml skhqtvqlpk egqpdagltk dytnsplhrf kkpgsknyqn iyppsstlhl
      481 snipttvtee eirdaftkng ftvkafkffp kdrkmaliqm psmddavaal ikmhnyqlse
      541 snhlrvsfsk sni
//
LOCUS       XP_034185205             534 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X10 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185205
VERSION     XP_034185205.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329314.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X10"
                     /calculated_mol_wt=58179
     Region          28..534
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..534
                     /gene="heph"
                     /coded_by="XM_034329314.2:790..2394"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mspqphhaad nnndakkvkl dkshngkpsr vihirnipne vsegeivhlg vpfgrvtnvl
       61 vlkgknqafl emadenaaat mvsyyascia qlrgravyvq fsnhrelktd qthannansn
      121 nqvalpgqnq vaqtqaetqg gpntvlrviv eqmvypisld vlyqiftrfg kvlkivtftk
      181 nstfqaliqy admlsaqtak lslegqniyn scctlridys kmqnlnvkyn ndksrdytnp
      241 slptgdanld aaslalggel lpqllmgags qpraripves iagapgvlpt pfaamhglps
      301 plagpyngvp pagglaglgg fplgaaglgv rvptnaqtsa vllvsnlnee mvtpdalftl
      361 fgvygdvqrv kilynkkdsa liqmaephqa hlalthmdkl rvfgkqikvm lskhqtvqlp
      421 kegqpdaglt kdytnsplhr fkkpgsknyq niyppsstlh lsnipttvte eeirdaftkn
      481 gftvkafkff pkdrkmaliq mpsmddavaa likmhnyqls esnhlrvsfs ksni
//
LOCUS       XP_034185208             534 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X10 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185208
VERSION     XP_034185208.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329317.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X10"
                     /calculated_mol_wt=58179
     Region          28..534
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..534
                     /gene="heph"
                     /coded_by="XM_034329317.2:159..1763"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mspqphhaad nnndakkvkl dkshngkpsr vihirnipne vsegeivhlg vpfgrvtnvl
       61 vlkgknqafl emadenaaat mvsyyascia qlrgravyvq fsnhrelktd qthannansn
      121 nqvalpgqnq vaqtqaetqg gpntvlrviv eqmvypisld vlyqiftrfg kvlkivtftk
      181 nstfqaliqy admlsaqtak lslegqniyn scctlridys kmqnlnvkyn ndksrdytnp
      241 slptgdanld aaslalggel lpqllmgags qpraripves iagapgvlpt pfaamhglps
      301 plagpyngvp pagglaglgg fplgaaglgv rvptnaqtsa vllvsnlnee mvtpdalftl
      361 fgvygdvqrv kilynkkdsa liqmaephqa hlalthmdkl rvfgkqikvm lskhqtvqlp
      421 kegqpdaglt kdytnsplhr fkkpgsknyq niyppsstlh lsnipttvte eeirdaftkn
      481 gftvkafkff pkdrkmaliq mpsmddavaa likmhnyqls esnhlrvsfs ksni
//
LOCUS       XP_076545708             578 aa            linear   INV 03-SEP-2025
DEFINITION  polypyrimidine tract-binding protein 1 heph isoform X11 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545708
VERSION     XP_076545708.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689593.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="polypyrimidine tract-binding protein 1 heph
                     isoform X11"
                     /calculated_mol_wt=62709
     Region          73..578
                     /region_name="hnRNP-L_PTB"
                     /note="hnRNP-L/PTB/hephaestus splicing factor family;
                     TIGR01649"
                     /db_xref="CDD:273733"
     CDS             1..578
                     /gene="heph"
                     /coded_by="XM_076689593.1:888..2624"
                     /db_xref="GeneID:117606618"
ORIGIN      
        1 mmmanlppvm lppltdygml tptlgikrgs dellsqsgav sngaamspqp hhaadnnnda
       61 kkvkldkshn gkpsrvihir nipnevsege ivhlgvpfgr vtnvlvlkgk nqaflemade
      121 naaatmvsyy asciaqlrgr avyvqfsnhr elktdqthan nansnnqval pgqnqvaqtq
      181 aetqggpntv lrviveqmvy pisldvlyqi ftrfgkvlki vtftknstfq aliqyadmls
      241 aqtaklsleg qniynscctl ridyskmqnl nvkynndksr dytnpslptg danldaasla
      301 lggellpqll mgagsqprar ipesiagapg vlptpfaamh glpsplagpy ngvppaggla
      361 glggfplgaa glgvrvptna qtsavllvsn lneemvtpda lftlfgvygd vqrvkilynk
      421 kdsaliqmae phqahlalth mdklrvfgkq ikvmlskhqt vqlpkegqpd agltkdytns
      481 plhrfkkpgs knyqniypps stlhlsnipt tvteeeirda ftkngftvka fkffpkdrkm
      541 aliqmpsmdd avaalikmhn yqlsesnhlr vsfsksni
//
LOCUS       XP_076545709             632 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305553 [Osmia lignaria lignaria].
ACCESSION   XP_076545709
VERSION     XP_076545709.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689594.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..632
                     /product="uncharacterized protein LOC143305553"
                     /calculated_mol_wt=72031
     Region          <93..181
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          308..524
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(373..378,412..413,448,450..451,520..521)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(373..378,412,450)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            413
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          539..>600
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..632
                     /gene="LOC143305553"
                     /coded_by="XM_076689594.1:1..1899"
                     /db_xref="GeneID:143305553"
ORIGIN      
        1 meltikaktq msrvemlrri mdkmpemlde ttpediesis vqldeihklf qkehpylemt
       61 wpasqashpy lkddyyelem gvvlevrhrl vliqthilrt ltatlpssea pkedwphlnr
      121 lkladpdfyk prsidiiiga dnygriikpn lvkgspsmpv aqlsifgwlv igsastrqar
      181 ayssfnasis qdsdaalrel ltkfwiqeep ptettsqltp eeqecenhfl athgrdstgr
      241 yvariplksn tkvlgtsyst ahfclrrtln klsrnpeyre qyqrfmreye dlghmkkafs
      301 ispndtsiyy lphhgvfkpd seatklrvvf ngssptttgi svndimhtga nlllnitdvl
      361 lwirhhkcif atditkmyrq vrvheddwnl qrilwidees nevpytlttv tygtkaapfl
      421 avrallqlae degqryplav psikyaryvd difggadsld slieiasqlt dlcnaggfpl
      481 akwhsneasl leaivpcnns qstsiflddc ntkilglrwn tvndsftftt kshhnlnftk
      541 rlvlsevaqi fdplgflspv iirakvllqe lwllnlkwdd plpshvttrw tairedltsl
      601 aysqyqggst pttilqwnct vsltlhnspw qq
//
LOCUS       XP_076545710             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305554 [Osmia lignaria lignaria].
ACCESSION   XP_076545710
VERSION     XP_076545710.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689595.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC143305554"
                     /calculated_mol_wt=54422
     Region          <1..147
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            37
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          163..325
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..479
                     /gene="LOC143305554"
                     /coded_by="XM_076689595.1:1..1440"
                     /db_xref="GeneID:143305554"
ORIGIN      
        1 myrqvavhkd dwdlqrilwi dedrnvipyq lttvtygtka apflttralm qlvhdeghrf
       61 platpslthg ryvddifgga nsvtelgeva qqlialcyag gfplakwhat hpdllravss
      121 stqssalisf ddcttkllgi qwmpqtdtfg flstitdqps kcskrlvlse varifdplgf
      181 vspvivrakm llqelwlhki nwdnplpsqi isrwfiired lnslaklsip rwfntwsnst
      241 vdihgfcdas qlamaavihi tvsspsnhst tsivcsktkv aplkrltipr lelsaallla
      301 klakytyvqt tlkvkintth lwtdsqvslt wiksqvsrwk dyvrnrdiqi qeltpnahwk
      361 hvpgasnpad casrgistdq lqrlelwwkg plwmtldqdh wpeqkkfstl tsplevrpnv
      421 slfasaqkls yhwdliykys sliklyrlta lcfrfasrlr rkleaplvii vpscdmeka
//
LOCUS       XP_076545711             358 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305555 [Osmia lignaria lignaria].
ACCESSION   XP_076545711
VERSION     XP_076545711.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689596.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 14% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="uncharacterized protein LOC143305555"
                     /calculated_mol_wt=41021
     CDS             1..358
                     /gene="LOC143305555"
                     /coded_by="XM_076689596.1:83..1159"
                     /db_xref="GeneID:143305555"
ORIGIN      
        1 megghtfwsa lpredcfktk ydvlyrapvt kltsnnretl ytiettdisf alaakgdikt
       61 cnriltkteh pkliiyertq nnilfdndvq pskeilnldi ltytnskfvy vekhfraqiq
      121 nlyldilser cklekrtiyn slsiasispd efaynlmekp gyiatiagev vhiikctpkk
      181 vqllhtdkcy tqlpvtsgnq trfltpkthi lmksgtqiec ntiipqyykl nnnwialtpt
      241 prkaqnpgil kpntniswey keinslaasg iysqedlqel qqhimfplek saqlnttarg
      301 mlgeesvqgg linnlftent lshiaentwn kftekfiknn knikkqevdd kqqrktsn
//
LOCUS       XP_034195248             223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611416 [Osmia lignaria lignaria].
ACCESSION   XP_034195248
VERSION     XP_034195248.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339357.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195248.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="uncharacterized protein LOC117611416"
                     /calculated_mol_wt=24831
     Region          13..101
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(27,29,31,51..53,90)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133158"
     Site            27..29
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133158"
     Site            27
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133158"
     Site            order(51..54,58..61)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133158"
     CDS             1..223
                     /gene="LOC117611416"
                     /coded_by="XM_034339357.2:1..672"
                     /db_xref="GeneID:117611416"
ORIGIN      
        1 mtaadglpnn crlvvkdcls nlrflidiga dnsalphrsa krestpsdfk iyaangtviq
       61 tygttrlvln lglrrpflwe fivaniqqpi igadflrkhg llvdlrnrkl idgltklhtv
      121 gqvstipqls lttidnnnpy qdllrqfihv trtiqchtep khkvrhhiit kdapcaerar
      181 rlptekfkaa qaeinhliaq gicilssssw vsplhmvpkk ngg
//
LOCUS       XP_034195247             469 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611288 [Osmia lignaria lignaria].
ACCESSION   XP_034195247
VERSION     XP_034195247.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339356.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195247.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="uncharacterized protein LOC117611288"
                     /calculated_mol_wt=52055
     Region          1..202
                     /region_name="R1-I-EN"
                     /note="Endonuclease domain encoded by various R1- and
                     I-clade non-long terminal repeat retrotransposons;
                     cd09077"
                     /db_xref="CDD:197311"
     Site            order(7,9,34,88,124,126,199)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(7,34,88,124,126,173,198..199)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(34,198)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..469
                     /gene="LOC117611288"
                     /coded_by="XM_034339356.2:1..1410"
                     /db_xref="GeneID:117611288"
ORIGIN      
        1 mkilqaninh crlahdmllv satekiadlv vvaepytipd nwfsdttgra aifitntgla
       61 kckqttivgc gigyvgisfn glgiisvytt pnanpqevev qfsalealvr dlqsshskil
      121 mlgdfnaksp lwgadrwcgr gkvlfqlats lglhptiavg gatcergggs vvdimlgndl
      181 alsphissev sdeftgsdhr yvwhsfresd svyavdhdlf dlgkgkidee rllaalikky
      241 gdeslerrgr tgtthevdkf iedlerlvnr yttyrratlr ahkpaywwnd avavkrkevn
      301 karrkytrar aknvtadtea ayaiykaakk alnrevnkak ktnwrellse idkdiwgrpy
      361 ktvmkklkgk ttkpdvmcpe eveavveklf ilkppdgqlp vnkgleinsv etagvgylhd
      421 ssylpavrht ngqhlsvsph ihrhtaisdv htapnviafh sndtykhla
//
LOCUS       XP_076545712            1101 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305557 [Osmia lignaria lignaria].
ACCESSION   XP_076545712
VERSION     XP_076545712.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689597.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1101
                     /product="uncharacterized protein LOC143305557"
                     /calculated_mol_wt=125463
     Region          78..>118
                     /region_name="Retrotrans_gag"
                     /note="Retrotransposon gag protein; cl46289"
                     /db_xref="CDD:480629"
     Region          <221..299
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          319..393
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(322,324,326,349..351,391)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            322..324
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            322
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(349..352,356..359)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          507..682
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(569..574,603..604,631,633..634,678..679)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(569..574,603,633)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            604
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          776..895
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(780..783,816..818,821,846,879,893)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     Region          <941..>963
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     CDS             1..1101
                     /gene="LOC143305557"
                     /coded_by="XM_076689597.1:1..3306"
                     /db_xref="GeneID:143305557"
ORIGIN      
        1 mpsepgtsvp psfdelfrrf metvasgavs tvtsatsnka tadaipefsg tdlgedanqw
       61 cdivekitdk lpisqrlsla thalhgaakk wykgwegnpr twttfredlc svfvsedrlc
      121 erlscavnyn sdsaisysey vrnklkyygq tqisfkphel islvighvtd psvrqslmna
      181 rytttadlls gisnfvkvpr keknsdskga vykrrdqilr rrcyqcnetg hlqhdcprkr
      241 kaeslpkdpv krtnvptctf cskrgheese cwlkrrtpre dkgakktnsr hgeleanacf
      301 rldhqltpii lqdmvvrscl vdngatcslv keqvakrahc kidpyitivr glcdsavttv
      361 gqttaviqte evtaeldllv vsedtmtydv iigknivtqg iqiltnsege tklqrcslvp
      421 qgiqedstpq cftcdvgdvg vrgeveklld rynhmitsgn kvrcvstsel kiklkedkvi
      481 nyhpyrlsis erekvreiid dllanniire saspfaspii lvkkkngkdr mcvdfralnr
      541 itvkdryplp liedqldrlg rgkyytildm tsgfyqisia edsiektafv tpdghyeflr
      601 mpfglanapa vfqraintal gtlrnkialv yiddvlipsr tieeglkyle ivlqaldtag
      661 fsiniekckf fqktieylgr evsaegirpg kdkvralles pvpcnikqvr qfvglasyfr
      721 kfvpefavrt acitkltkkn eqwhwaeeqe karhyilekl ssnpllvifn peretelhtd
      781 asaigygail fqkvdgdsrv vayfsrrttp eesryhsyel etlaivnalr hfrvyllgik
      841 fkiitdcnai katstkkdll prvarwwifl qdfnfeieyr kgkyvehvdy lsrnppkdyr
      901 vnticegswl evaqrkdeet ksiierietn dtvssdfevr qgpektydri mqkywfpkmr
      961 kvvvmdratn ftfkplvqfi kkhgidlhfi atgaprangq aerihsrfdk krrvnvvyke
     1021 gdtvfvkpad lrraklelky vgpfvvskil andryeivgk tgrpqtvpkd rlrlwkgews
     1081 gdtdtnegln desdsgdepc n
//
LOCUS       XP_076545713            1228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611335 [Osmia lignaria lignaria].
ACCESSION   XP_076545713
VERSION     XP_076545713.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689598.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1228
                     /product="uncharacterized protein LOC117611335"
                     /calculated_mol_wt=137307
     Region          236..447
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(300..305,340..341,376,378..379,438..439)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(300..305,340,378)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            341
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          464..623
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          844..894
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1122..1215
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1228
                     /gene="LOC117611335"
                     /coded_by="XM_076689598.1:1..3687"
                     /db_xref="GeneID:117611335"
ORIGIN      
        1 maspaksapt akgdaptspp pavfsdieek nvaasfacsa ttrpaallat akvrveapsg
       61 ehlevralld tgsdtsldes rvgpmgtpta rrtplgwvlm gpvsdakgps skavvhslhc
      121 qtddstnrll rrfweleelh eqpplspedr ecerhfreth yrdetgrysv hlpfkldpsl
      181 alkpsrstal klllscerkl asneawrdky sqfmdeyaal nhmeavpesa vqdpayylph
      241 havakrydpt gkirvvfnas frtatgssln dcllsgpklq sdlwmiltrw rlfrvvftsd
      301 ivkmfrqirv dpadadwtri lwrsrpdqpv qdfrlktvty gtacapflal rvlvqlaade
      361 eerfplgava irrhsyvddi lagaddeeaa lelrrqvisi lqsggfdlsk wasnkpslqe
      421 aeesqacvfq dhtgvstlgv vwrpttdtfs lrvlpplkep srytkrrils evaslfdplg
      481 waapvlifak ilmqdlwiig aewdedlpdq llsawktfrt tlsqldalai prwtnysads
      541 aqldlhgfcd asqrayaavv ylrvskgevs vttllvaktk vapvkavsip rlelcgavll
      601 skliaevqrg lnvpvtitaw tdssvtlhwi rghastwkpf vahrvatvqs nvppqnwrhv
      661 atkhnpadla trgitpsell ssqlwwrgps wlinppdqwp asplgkveea dleqgrvqiy
      721 lakeneendl ltrfssltrl tavialcfrf hrltrklkte tgfiraserd aalriaigla
      781 qksnfhaevd qlrqhkevrr sstlialrpf lddqgllrvg grldqaslpy nerhpyiigk
      841 knplatllvr dahlrtlhgg pqltrsllar rywiihgrsl vrdvikgcvk carysgrptt
      901 qlmgplpier itprrpflst gvdyagpvkl rtspgrghks ykgyialfic lstraihlea
      961 vsdltsasfl aafrrfisrr grcatmlsdn gtvfhgadre lrnmfrgasa fylnaaasla
     1021 aegtdwsfip pyaphfgglw eagvrtvkhh lrrivenatl tfeemttllc qveacvnsrp
     1081 lhplsddpsd lsaltpghfl igeptcivpd pesdshdisl isrwrqisal rshfwhrwrr
     1141 eylqhlqqlp kwrqqkenlk lgtlvllrde lqppakwpmg rvealhpgpd grvrvatlkt
     1201 tsgfvtrpiv klcplpvpsa dvtsptst
//
LOCUS       XP_034195126             154 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611281 [Osmia lignaria lignaria].
ACCESSION   XP_034195126
VERSION     XP_034195126.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195126.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..154
                     /product="uncharacterized protein LOC117611281"
                     /calculated_mol_wt=17443
     CDS             1..154
                     /gene="LOC117611281"
                     /coded_by="XM_034339235.2:255..719"
                     /db_xref="GeneID:117611281"
ORIGIN      
        1 mrlhsplsll hnltnlvrny qcyqqcdnpn llvpppeklk frvirssyfe ekyfmrkrlt
       61 tsavptlhlp vseaasikte qqetrmeipe eeeeckpdct ggmdtksgiv rhvdftalpg
      121 psgemqcndl grlslqedks lrnadcavlg rehq
//
LOCUS       XP_034195125             253 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611280 [Osmia lignaria lignaria].
ACCESSION   XP_034195125
VERSION     XP_034195125.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339234.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195125.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="uncharacterized protein LOC117611280"
                     /calculated_mol_wt=28459
     Region          96..>217
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     CDS             1..253
                     /gene="LOC117611280"
                     /coded_by="XM_034339234.2:18..779"
                     /db_xref="GeneID:117611280"
ORIGIN      
        1 magrdwvysf lkrhpelklr qstptsvara mgfnqtqvnt fydnlqslyn kykfppsrif
       61 nmdesgintv pkkipkvism kgkklvgkii laergqtitl vcamnvtgny vppafifprk
      121 rmkgyllnna pegsigmvsd sgfintdlfi eyvhhfkenv qptkdnsvll ildnhsshls
      181 laaidycrns gihlltlpph sshkmqpldr gffgplkikf ayecdkwlsh hpgrvigqte
      241 vaylgfskss ngr
//
LOCUS       XP_034185331            2443 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185331
VERSION     XP_034185331.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329440.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185331.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2443
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X1"
                     /calculated_mol_wt=268804
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1451..>1693
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1724..1758
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1794..2001
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2010..2256
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2279..2348
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2443
                     /gene="gw"
                     /coded_by="XM_034329440.2:179..7510"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrav gmqcppgmpq
     1621 nrmpgqpgmk pdvsgpmwgh pgapggrngs waegshdags wdepktpstw neaplnpgtw
     1681 gapsthkpkp mgptgswvdt dmdptpswgh ptkptltkev iwnsrefryl cdlgfkkedv
     1741 elalrnremn reealellsq lrpvdqwrrh dahssydptn qattapaypr fnhvaqqmsf
     1801 ppgagvpsgn ttgsvggsva sasllklqqq qqqaavplqq qqpstnapqp pfnqasrapq
     1861 nqpstqqlrm lvqqiqlavq egylnhqiln qplapqtlil lnqllqqikv lqqlhqqhsv
     1921 qstmkgngqs vlqisvqitk tkqqianlqn qiavqqatym kqqqqqqqqq qqqqqqhpap
     1981 psqsseyyks svhdpmsalq nsftdltmnk eppvsqqqsr lnqwklpsld kdgelvtnef
     2041 srapgttskp aatpagltrs hsspnmnpll gqgdgtwstr lgesgwpdpg ntdstdgkdw
     2101 qptgaaagaa aftdlvpefe pgkpwkgtqm ksieddpsit pgsvvrspls laaikdpdai
     2161 fssssktspp pqqptnpdts ipslsnstwt fnppattpsa ftrrimfsak ntwgesappp
     2221 tavtselwga pmskvrgppp glsskatgnt sngwaglgtv grsssswglq sstnagwvst
     2281 wlllknltpq idgstlktlc mqhgpvqdfr lylnhgialt kyssrdeaik aqgalnncvl
     2341 gnttifaesp adsevhtllq qlshggqqqa gatagagwsl rpsnktgppp dtwggsssql
     2401 wgappssnsl wsstgidsnd qqratpssln sylpgdllgg esm
//
LOCUS       XP_034185332            2442 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034185332
VERSION     XP_034185332.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329441.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185332.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2442
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X2"
                     /calculated_mol_wt=268705
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1505..>1695
                     /region_name="dnaA"
                     /note="chromosomal replication initiator protein DnaA;
                     cl42516"
                     /db_xref="CDD:455861"
     Region          1723..1757
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1793..2000
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2009..2255
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2278..2347
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2442
                     /gene="gw"
                     /coded_by="XM_034329441.2:179..7507"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrag mqcppgmpqn
     1621 rmpgqpgmkp dvsgpmwghp gapggrngsw aegshdagsw depktpstwn eaplnpgtwg
     1681 apsthkpkpm gptgswvdtd mdptpswghp tkptltkevi wnsrefrylc dlgfkkedve
     1741 lalrnremnr eealellsql rpvdqwrrhd ahssydptnq attapayprf nhvaqqmsfp
     1801 pgagvpsgnt tgsvggsvas asllklqqqq qqaavplqqq qpstnapqpp fnqasrapqn
     1861 qpstqqlrml vqqiqlavqe gylnhqilnq plapqtlill nqllqqikvl qqlhqqhsvq
     1921 stmkgngqsv lqisvqitkt kqqianlqnq iavqqatymk qqqqqqqqqq qqqqqhpapp
     1981 sqsseyykss vhdpmsalqn sftdltmnke ppvsqqqsrl nqwklpsldk dgelvtnefs
     2041 rapgttskpa atpagltrsh sspnmnpllg qgdgtwstrl gesgwpdpgn tdstdgkdwq
     2101 ptgaaagaaa ftdlvpefep gkpwkgtqmk sieddpsitp gsvvrsplsl aaikdpdaif
     2161 ssssktsppp qqptnpdtsi pslsnstwtf nppattpsaf trrimfsakn twgesapppt
     2221 avtselwgap mskvrgpppg lsskatgnts ngwaglgtvg rsssswglqs stnagwvstw
     2281 lllknltpqi dgstlktlcm qhgpvqdfrl ylnhgialtk yssrdeaika qgalnncvlg
     2341 nttifaespa dsevhtllqq lshggqqqag atagagwslr psnktgpppd twggsssqlw
     2401 gappssnslw sstgidsndq qratpsslns ylpgdllgge sm
//
LOCUS       XP_034185333            2440 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034185333
VERSION     XP_034185333.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329442.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185333.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2440
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X3"
                     /calculated_mol_wt=268518
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1451..>1693
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1724..1758
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1794..2001
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2010..2253
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2276..2345
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2440
                     /gene="gw"
                     /coded_by="XM_034329442.2:179..7501"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrav gmqcppgmpq
     1621 nrmpgqpgmk pdvsgpmwgh pgapggrngs waegshdags wdepktpstw neaplnpgtw
     1681 gapsthkpkp mgptgswvdt dmdptpswgh ptkptltkev iwnsrefryl cdlgfkkedv
     1741 elalrnremn reealellsq lrpvdqwrrh dahssydptn qattapaypr fnhvaqqmsf
     1801 ppgagvpsgn ttgsvggsva sasllklqqq qqqaavplqq qqpstnapqp pfnqasrapq
     1861 nqpstqqlrm lvqqiqlavq egylnhqiln qplapqtlil lnqllqqikv lqqlhqqhsv
     1921 qstmkgngqs vlqisvqitk tkqqianlqn qiavqqatym kqqqqqqqqq qqqqqqhpap
     1981 psqsseyyks svhdpmsalq nsftdltmnk eppvsqqqsr lnqwklpsld kdgelvtnef
     2041 srapgttskp aatpagltrs hsspnmnpll gqgdgtwstr lgesgwpdpg ntdstdgkdw
     2101 qptgaaagaa aftdlvpefe pgkpwkmksi eddpsitpgs vvrsplslaa ikdpdaifss
     2161 ssktspppqq ptnpdtsips lsnstwtfnp pattpsaftr rimfsakntw gesappptav
     2221 tselwgapms kvrgpppgls skatgntsng waglgtvgrs ssswglqsst nagwvstwll
     2281 lknltpqidg stlktlcmqh gpvqdfrlyl nhgialtkys srdeaikaqg alnncvlgnt
     2341 tifaespads evhtllqqls hggqqqagat agagwslrps nktgpppdtw ggsssqlwga
     2401 ppssnslwss tgidsndqqr atpsslnsyl pgdllggesm
//
LOCUS       XP_034185334            2438 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034185334
VERSION     XP_034185334.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329443.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185334.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2438
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X4"
                     /calculated_mol_wt=268100
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1451..>1693
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1724..1758
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1794..2001
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2010..2251
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2274..2343
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2438
                     /gene="gw"
                     /coded_by="XM_034329443.2:178..7494"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrav gmqcppgmpq
     1621 nrmpgqpgmk pdvsgpmwgh pgapggrngs waegshdags wdepktpstw neaplnpgtw
     1681 gapsthkpkp mgptgswvdt dmdptpswgh ptkptltkev iwnsrefryl cdlgfkkedv
     1741 elalrnremn reealellsq lrpvdqwrrh dahssydptn qattapaypr fnhvaqqmsf
     1801 ppgagvpsgn ttgsvggsva sasllklqqq qqqaavplqq qqpstnapqp pfnqasrapq
     1861 nqpstqqlrm lvqqiqlavq egylnhqiln qplapqtlil lnqllqqikv lqqlhqqhsv
     1921 qstmkgngqs vlqisvqitk tkqqianlqn qiavqqatym kqqqqqqqqq qqqqqqhpap
     1981 psqsseyyks svhdpmsalq nsftdltmnk eppvsqqqsr lnqwklpsld kdgelvtnef
     2041 srapgttskp aatpagltrs hsspnmnpll gqgdgtwstr lgesgwpdpg ntdstdgkdw
     2101 qptgaaagaa aftdlvpefe pgkpwkgtqm ksieddpsit pgsvvrspls laaikdpdai
     2161 fssssktspp pqqptnpdts ipslsnstwt fnppattpsa ftsakntwge sappptavts
     2221 elwgapmskv rgpppglssk atgntsngwa glgtvgrsss swglqsstna gwvstwlllk
     2281 nltpqidgst lktlcmqhgp vqdfrlylnh gialtkyssr deaikaqgal nncvlgntti
     2341 faespadsev htllqqlshg gqqqagatag agwslrpsnk tgpppdtwgg sssqlwgapp
     2401 ssnslwsstg idsndqqrat psslnsylpg dllggesm
//
LOCUS       XP_034185335            2437 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034185335
VERSION     XP_034185335.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329444.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185335.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2437
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X5"
                     /calculated_mol_wt=268001
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1505..>1695
                     /region_name="dnaA"
                     /note="chromosomal replication initiator protein DnaA;
                     cl42516"
                     /db_xref="CDD:455861"
     Region          1723..1757
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1793..2000
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2009..2250
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2273..2342
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2437
                     /gene="gw"
                     /coded_by="XM_034329444.2:178..7491"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrag mqcppgmpqn
     1621 rmpgqpgmkp dvsgpmwghp gapggrngsw aegshdagsw depktpstwn eaplnpgtwg
     1681 apsthkpkpm gptgswvdtd mdptpswghp tkptltkevi wnsrefrylc dlgfkkedve
     1741 lalrnremnr eealellsql rpvdqwrrhd ahssydptnq attapayprf nhvaqqmsfp
     1801 pgagvpsgnt tgsvggsvas asllklqqqq qqaavplqqq qpstnapqpp fnqasrapqn
     1861 qpstqqlrml vqqiqlavqe gylnhqilnq plapqtlill nqllqqikvl qqlhqqhsvq
     1921 stmkgngqsv lqisvqitkt kqqianlqnq iavqqatymk qqqqqqqqqq qqqqqhpapp
     1981 sqsseyykss vhdpmsalqn sftdltmnke ppvsqqqsrl nqwklpsldk dgelvtnefs
     2041 rapgttskpa atpagltrsh sspnmnpllg qgdgtwstrl gesgwpdpgn tdstdgkdwq
     2101 ptgaaagaaa ftdlvpefep gkpwkgtqmk sieddpsitp gsvvrsplsl aaikdpdaif
     2161 ssssktsppp qqptnpdtsi pslsnstwtf nppattpsaf tsakntwges appptavtse
     2221 lwgapmskvr gpppglsska tgntsngwag lgtvgrssss wglqsstnag wvstwlllkn
     2281 ltpqidgstl ktlcmqhgpv qdfrlylnhg ialtkyssrd eaikaqgaln ncvlgnttif
     2341 aespadsevh tllqqlshgg qqqagataga gwslrpsnkt gpppdtwggs ssqlwgapps
     2401 snslwsstgi dsndqqratp sslnsylpgd llggesm
//
LOCUS       XP_034185336            2435 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X6
            [Osmia lignaria lignaria].
ACCESSION   XP_034185336
VERSION     XP_034185336.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329445.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185336.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2435
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X6"
                     /calculated_mol_wt=267814
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1451..>1693
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1724..1758
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1794..2001
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2010..2248
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2271..2340
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2435
                     /gene="gw"
                     /coded_by="XM_034329445.2:179..7486"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrav gmqcppgmpq
     1621 nrmpgqpgmk pdvsgpmwgh pgapggrngs waegshdags wdepktpstw neaplnpgtw
     1681 gapsthkpkp mgptgswvdt dmdptpswgh ptkptltkev iwnsrefryl cdlgfkkedv
     1741 elalrnremn reealellsq lrpvdqwrrh dahssydptn qattapaypr fnhvaqqmsf
     1801 ppgagvpsgn ttgsvggsva sasllklqqq qqqaavplqq qqpstnapqp pfnqasrapq
     1861 nqpstqqlrm lvqqiqlavq egylnhqiln qplapqtlil lnqllqqikv lqqlhqqhsv
     1921 qstmkgngqs vlqisvqitk tkqqianlqn qiavqqatym kqqqqqqqqq qqqqqqhpap
     1981 psqsseyyks svhdpmsalq nsftdltmnk eppvsqqqsr lnqwklpsld kdgelvtnef
     2041 srapgttskp aatpagltrs hsspnmnpll gqgdgtwstr lgesgwpdpg ntdstdgkdw
     2101 qptgaaagaa aftdlvpefe pgkpwkmksi eddpsitpgs vvrsplslaa ikdpdaifss
     2161 ssktspppqq ptnpdtsips lsnstwtfnp pattpsafts akntwgesap pptavtselw
     2221 gapmskvrgp ppglsskatg ntsngwaglg tvgrsssswg lqsstnagwv stwlllknlt
     2281 pqidgstlkt lcmqhgpvqd frlylnhgia ltkyssrdea ikaqgalnnc vlgnttifae
     2341 spadsevhtl lqqlshggqq qagatagagw slrpsnktgp ppdtwggsss qlwgappssn
     2401 slwsstgids ndqqratpss lnsylpgdll ggesm
//
LOCUS       XP_076545714            2434 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X7
            [Osmia lignaria lignaria].
ACCESSION   XP_076545714
VERSION     XP_076545714.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689599.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2434
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X7"
                     /calculated_mol_wt=267715
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1505..>1695
                     /region_name="dnaA"
                     /note="chromosomal replication initiator protein DnaA;
                     cl42516"
                     /db_xref="CDD:455861"
     Region          1723..1757
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1793..2000
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2009..2247
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2270..2339
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2434
                     /gene="gw"
                     /coded_by="XM_076689599.1:179..7483"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrag mqcppgmpqn
     1621 rmpgqpgmkp dvsgpmwghp gapggrngsw aegshdagsw depktpstwn eaplnpgtwg
     1681 apsthkpkpm gptgswvdtd mdptpswghp tkptltkevi wnsrefrylc dlgfkkedve
     1741 lalrnremnr eealellsql rpvdqwrrhd ahssydptnq attapayprf nhvaqqmsfp
     1801 pgagvpsgnt tgsvggsvas asllklqqqq qqaavplqqq qpstnapqpp fnqasrapqn
     1861 qpstqqlrml vqqiqlavqe gylnhqilnq plapqtlill nqllqqikvl qqlhqqhsvq
     1921 stmkgngqsv lqisvqitkt kqqianlqnq iavqqatymk qqqqqqqqqq qqqqqhpapp
     1981 sqsseyykss vhdpmsalqn sftdltmnke ppvsqqqsrl nqwklpsldk dgelvtnefs
     2041 rapgttskpa atpagltrsh sspnmnpllg qgdgtwstrl gesgwpdpgn tdstdgkdwq
     2101 ptgaaagaaa ftdlvpefep gkpwkmksie ddpsitpgsv vrsplslaai kdpdaifsss
     2161 sktspppqqp tnpdtsipsl snstwtfnpp attpsaftsa kntwgesapp ptavtselwg
     2221 apmskvrgpp pglsskatgn tsngwaglgt vgrsssswgl qsstnagwvs twlllknltp
     2281 qidgstlktl cmqhgpvqdf rlylnhgial tkyssrdeai kaqgalnncv lgnttifaes
     2341 padsevhtll qqlshggqqq agatagagws lrpsnktgpp pdtwggsssq lwgappssns
     2401 lwsstgidsn dqqratpssl nsylpgdllg gesm
//
LOCUS       XP_034185337            2418 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X8
            [Osmia lignaria lignaria].
ACCESSION   XP_034185337
VERSION     XP_034185337.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329446.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185337.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2418
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X8"
                     /calculated_mol_wt=266388
     Region          <1127..>1251
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1451..>1693
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1724..1758
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1794..2001
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          2010..2231
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2254..2323
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2418
                     /gene="gw"
                     /coded_by="XM_034329446.2:179..7435"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mviysdlhwh garstliviv ykvhcfqtld lgfyalpake elspmfphns ssheistetn
       61 afvqnsmgdv vvlgknspig mgegresena rycdiefinn smmakptshl enivatdflt
      121 vlssqtgkfi ltsrslicqs vassttkhpt knhlltydnn niiininnnn nnngssgnnn
      181 nnndndnpkl ndrsnraehn hqddrkptvh ktttttttkr khpgmsgnkk pvssannsks
      241 msktlsdnkt sfsvlnirvl sisvnlrltg dkcavgegvp slvripksdr pssghfslds
      301 ldnysllgsc lhsgkynnvk snfsllsnnn dnyksvills stsnhyflrv emdgganamk
      361 lrthldlfry fvksffdgls lqkqynfill peqdddeynf lindqmiksk hrwiinvqsi
      421 ngsrkfslty lelfsskilm eiiircvknd afschnqdsn elclsrlctf vkylypigvd
      481 skieiflsty lyskieslrl vysdnlktls einsvfveil lpavacsesn khpmmkscli
      541 redetltnvv ivvsvkyris kaplnfiipr nnvyvrdtss neesrlikeg esvatsssit
      601 livpgneltd qpprpfplrn apnsaqssps wldlvaselt qracvplslg cklyvvkfsy
      661 kvicdfsitw kgipshetsy erthldvqfl sisvqinett ftykipnflr rtrtcllndd
      721 pleqgishdi flslirklta ttmncenmns mkdeqlnvll fnsirnlvec rmsivgmeny
      781 kicwsliitl rkllndeift siriltrnyv fllkifrrmy ysnvtlmneq qeqqqypndg
      841 cllkrtsdtl ifklkkktme mlssdingin rvatitvgal sfqdnvksne psatylasyk
      901 ysgshasysi sslrsnlfkr iklrcqafad vmndkisred lcdlyrfkrr lykvhevigy
      961 nvenvftksl inckqvalig tttttttpqt arfvddsydc nkntspdfkn kndtvrlrdr
     1021 sqyemsetvv rsgddttsee ktndknpipl rlesnetivn gskriqliay arnliaaslg
     1081 vsdgtlqaqk ndlkqtlkll fgsvydmdqp nleykkrwgi pgilrlaggg etslnsagat
     1141 nwgstqtstt nnnnnnqsgw ggssgnpsgn sgapgnwtgn svnrsvaanq npnqnqgpgg
     1201 qnvpgnvnkv nnpnqqsnqq sgpptsqssg taqggqnsnq wsqgksnnpg gpgqnssgqn
     1261 nnsssvqqqq qsnsssnnnq pnnqaqgsvn nnntsasntp stkqqleqln tmrealfsqd
     1321 gwgcqhvnqd tnwdvptspe psmtkdgvpm wkppvnngtd lweanlrngg qpppqqqakt
     1381 pwghtpatni ggtwgeddea adssnmwtga ptssqpnsaa gqwttstgnq sgmwagsnwg
     1441 dpridhrdpr dlrsvdprem rdprdhrmsl dprehmrvmd pmardpriad mrgdprgisg
     1501 rlnganadam wgqppgpphh qmghqhpsgp pakmlnpsni nqwaapppkd impgkpsgwe
     1561 epspptqrrn vpnyddgtsl wgnpaanqra ipgskvshwk dlpsnlgrav gmqcppgmpq
     1621 nrmpgqpgmk pdvsgpmwgh pgapggrngs waegshdags wdepktpstw neaplnpgtw
     1681 gapsthkpkp mgptgswvdt dmdptpswgh ptkptltkev iwnsrefryl cdlgfkkedv
     1741 elalrnremn reealellsq lrpvdqwrrh dahssydptn qattapaypr fnhvaqqmsf
     1801 ppgagvpsgn ttgsvggsva sasllklqqq qqqaavplqq qqpstnapqp pfnqasrapq
     1861 nqpstqqlrm lvqqiqlavq egylnhqiln qplapqtlil lnqllqqikv lqqlhqqhsv
     1921 qstmkgngqs vlqisvqitk tkqqianlqn qiavqqatym kqqqqqqqqq qqqqqqhpap
     1981 psqsseyyks svhdpmsalq nsftdltmnk eppvsqqqsr lnqwklpsld kdgelvtnef
     2041 srapgttrdg twstrlgesg wpdpgntdst dgkdwqptga aagaaaftdl vpefepgkpw
     2101 kgtqmksied dpsitpgsvv rsplslaaik dpdaifssss ktspppqqpt npdtsipsls
     2161 nstwtfnppa ttpsaftrri mfsakntwge sappptavts elwgapmskv rgpppglssk
     2221 atgntsngwa glgtvgrsss swglqsstna gwvstwlllk nltpqidgst lktlcmqhgp
     2281 vqdfrlylnh gialtkyssr deaikaqgal nncvlgntti faespadsev htllqqlshg
     2341 gqqqagatag agwslrpsnk tgpppdtwgg sssqlwgapp ssnslwsstg idsndqqrat
     2401 psslnsylpg dllggesm
//
LOCUS       XP_034185338            2399 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X9
            [Osmia lignaria lignaria].
ACCESSION   XP_034185338
VERSION     XP_034185338.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329447.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185338.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2399
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X9"
                     /calculated_mol_wt=263759
     Region          <1083..>1207
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1407..>1649
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1680..1714
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1750..1957
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          1966..2212
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2235..2304
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2399
                     /gene="gw"
                     /coded_by="XM_034329447.2:235..7434"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mfphnssshe istetnafvq nsmgdvvvlg knspigmgeg resenarycd iefinnsmma
       61 kptshleniv atdfltvlss qtgkfiltsr slicqsvass ttkhptknhl ltydnnniii
      121 ninnnnnnng ssgnnnnnnd ndnpklndrs nraehnhqdd rkptvhkttt ttttkrkhpg
      181 msgnkkpvss annsksmskt lsdnktsfsv lnirvlsisv nlrltgdkca vgegvpslvr
      241 ipksdrpssg hfsldsldny sllgsclhsg kynnvksnfs llsnnndnyk svillsstsn
      301 hyflrvemdg ganamklrth ldlfryfvks ffdglslqkq ynfillpeqd ddeynflind
      361 qmikskhrwi invqsingsr kfsltylelf sskilmeiii rcvkndafsc hnqdsnelcl
      421 srlctfvkyl ypigvdskie iflstylysk ieslrlvysd nlktlseins vfveillpav
      481 acsesnkhpm mksclirede tltnvvivvs vkyriskapl nfiiprnnvy vrdtssnees
      541 rlikegesva tsssitlivp gneltdqppr pfplrnapns aqsspswldl vaseltqrac
      601 vplslgckly vvkfsykvic dfsitwkgip shetsyerth ldvqflsisv qinettftyk
      661 ipnflrrtrt cllnddpleq gishdiflsl irkltattmn cenmnsmkde qlnvllfnsi
      721 rnlvecrmsi vgmenykicw sliitlrkll ndeiftsiri ltrnyvfllk ifrrmyysnv
      781 tlmneqqeqq qypndgcllk rtsdtlifkl kkktmemlss dinginrvat itvgalsfqd
      841 nvksnepsat ylasykysgs hasysisslr snlfkriklr cqafadvmnd kisredlcdl
      901 yrfkrrlykv hevigynven vftkslinck qvaligtttt tttpqtarfv ddsydcnknt
      961 spdfknkndt vrlrdrsqye msetvvrsgd dttseektnd knpiplrles netivngskr
     1021 iqliayarnl iaaslgvsdg tlqaqkndlk qtlkllfgsv ydmdqpnley kkrwgipgil
     1081 rlagggetsl nsagatnwgs tqtsttnnnn nnqsgwggss gnpsgnsgap gnwtgnsvnr
     1141 svaanqnpnq nqgpggqnvp gnvnkvnnpn qqsnqqsgpp tsqssgtaqg gqnsnqwsqg
     1201 ksnnpggpgq nssgqnnnss svqqqqqsns ssnnnqpnnq aqgsvnnnnt sasntpstkq
     1261 qleqlntmre alfsqdgwgc qhvnqdtnwd vptspepsmt kdgvpmwkpp vnngtdlwea
     1321 nlrnggqppp qqqaktpwgh tpatniggtw geddeaadss nmwtgaptss qpnsaagqwt
     1381 tstgnqsgmw agsnwgdpri dhrdprdlrs vdpremrdpr dhrmsldpre hmrvmdpmar
     1441 dpriadmrgd prgisgrlng anadamwgqp pgpphhqmgh qhpsgppakm lnpsninqwa
     1501 apppkdimpg kpsgweepsp ptqrrnvpny ddgtslwgnp aanqraipgs kvshwkdlps
     1561 nlgravgmqc ppgmpqnrmp gqpgmkpdvs gpmwghpgap ggrngswaeg shdagswdep
     1621 ktpstwneap lnpgtwgaps thkpkpmgpt gswvdtdmdp tpswghptkp tltkeviwns
     1681 refrylcdlg fkkedvelal rnremnreea lellsqlrpv dqwrrhdahs sydptnqatt
     1741 apayprfnhv aqqmsfppga gvpsgnttgs vggsvasasl lklqqqqqqa avplqqqqps
     1801 tnapqppfnq asrapqnqps tqqlrmlvqq iqlavqegyl nhqilnqpla pqtlillnql
     1861 lqqikvlqql hqqhsvqstm kgngqsvlqi svqitktkqq ianlqnqiav qqatymkqqq
     1921 qqqqqqqqqq qqhpappsqs seyykssvhd pmsalqnsft dltmnkeppv sqqqsrlnqw
     1981 klpsldkdge lvtnefsrap gttskpaatp agltrshssp nmnpllgqgd gtwstrlges
     2041 gwpdpgntds tdgkdwqptg aaagaaaftd lvpefepgkp wkgtqmksie ddpsitpgsv
     2101 vrsplslaai kdpdaifsss sktspppqqp tnpdtsipsl snstwtfnpp attpsaftrr
     2161 imfsakntwg esappptavt selwgapmsk vrgpppglss katgntsngw aglgtvgrss
     2221 sswglqsstn agwvstwlll knltpqidgs tlktlcmqhg pvqdfrlyln hgialtkyss
     2281 rdeaikaqga lnncvlgntt ifaespadse vhtllqqlsh ggqqqagata gagwslrpsn
     2341 ktgpppdtwg gsssqlwgap pssnslwsst gidsndqqra tpsslnsylp gdllggesm
//
LOCUS       XP_034185339            2399 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X9
            [Osmia lignaria lignaria].
ACCESSION   XP_034185339
VERSION     XP_034185339.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329448.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185339.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2399
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X9"
                     /calculated_mol_wt=263759
     Region          <1083..>1207
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1407..>1649
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1680..1714
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1750..1957
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          1966..2212
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2235..2304
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2399
                     /gene="gw"
                     /coded_by="XM_034329448.2:289..7488"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mfphnssshe istetnafvq nsmgdvvvlg knspigmgeg resenarycd iefinnsmma
       61 kptshleniv atdfltvlss qtgkfiltsr slicqsvass ttkhptknhl ltydnnniii
      121 ninnnnnnng ssgnnnnnnd ndnpklndrs nraehnhqdd rkptvhkttt ttttkrkhpg
      181 msgnkkpvss annsksmskt lsdnktsfsv lnirvlsisv nlrltgdkca vgegvpslvr
      241 ipksdrpssg hfsldsldny sllgsclhsg kynnvksnfs llsnnndnyk svillsstsn
      301 hyflrvemdg ganamklrth ldlfryfvks ffdglslqkq ynfillpeqd ddeynflind
      361 qmikskhrwi invqsingsr kfsltylelf sskilmeiii rcvkndafsc hnqdsnelcl
      421 srlctfvkyl ypigvdskie iflstylysk ieslrlvysd nlktlseins vfveillpav
      481 acsesnkhpm mksclirede tltnvvivvs vkyriskapl nfiiprnnvy vrdtssnees
      541 rlikegesva tsssitlivp gneltdqppr pfplrnapns aqsspswldl vaseltqrac
      601 vplslgckly vvkfsykvic dfsitwkgip shetsyerth ldvqflsisv qinettftyk
      661 ipnflrrtrt cllnddpleq gishdiflsl irkltattmn cenmnsmkde qlnvllfnsi
      721 rnlvecrmsi vgmenykicw sliitlrkll ndeiftsiri ltrnyvfllk ifrrmyysnv
      781 tlmneqqeqq qypndgcllk rtsdtlifkl kkktmemlss dinginrvat itvgalsfqd
      841 nvksnepsat ylasykysgs hasysisslr snlfkriklr cqafadvmnd kisredlcdl
      901 yrfkrrlykv hevigynven vftkslinck qvaligtttt tttpqtarfv ddsydcnknt
      961 spdfknkndt vrlrdrsqye msetvvrsgd dttseektnd knpiplrles netivngskr
     1021 iqliayarnl iaaslgvsdg tlqaqkndlk qtlkllfgsv ydmdqpnley kkrwgipgil
     1081 rlagggetsl nsagatnwgs tqtsttnnnn nnqsgwggss gnpsgnsgap gnwtgnsvnr
     1141 svaanqnpnq nqgpggqnvp gnvnkvnnpn qqsnqqsgpp tsqssgtaqg gqnsnqwsqg
     1201 ksnnpggpgq nssgqnnnss svqqqqqsns ssnnnqpnnq aqgsvnnnnt sasntpstkq
     1261 qleqlntmre alfsqdgwgc qhvnqdtnwd vptspepsmt kdgvpmwkpp vnngtdlwea
     1321 nlrnggqppp qqqaktpwgh tpatniggtw geddeaadss nmwtgaptss qpnsaagqwt
     1381 tstgnqsgmw agsnwgdpri dhrdprdlrs vdpremrdpr dhrmsldpre hmrvmdpmar
     1441 dpriadmrgd prgisgrlng anadamwgqp pgpphhqmgh qhpsgppakm lnpsninqwa
     1501 apppkdimpg kpsgweepsp ptqrrnvpny ddgtslwgnp aanqraipgs kvshwkdlps
     1561 nlgravgmqc ppgmpqnrmp gqpgmkpdvs gpmwghpgap ggrngswaeg shdagswdep
     1621 ktpstwneap lnpgtwgaps thkpkpmgpt gswvdtdmdp tpswghptkp tltkeviwns
     1681 refrylcdlg fkkedvelal rnremnreea lellsqlrpv dqwrrhdahs sydptnqatt
     1741 apayprfnhv aqqmsfppga gvpsgnttgs vggsvasasl lklqqqqqqa avplqqqqps
     1801 tnapqppfnq asrapqnqps tqqlrmlvqq iqlavqegyl nhqilnqpla pqtlillnql
     1861 lqqikvlqql hqqhsvqstm kgngqsvlqi svqitktkqq ianlqnqiav qqatymkqqq
     1921 qqqqqqqqqq qqhpappsqs seyykssvhd pmsalqnsft dltmnkeppv sqqqsrlnqw
     1981 klpsldkdge lvtnefsrap gttskpaatp agltrshssp nmnpllgqgd gtwstrlges
     2041 gwpdpgntds tdgkdwqptg aaagaaaftd lvpefepgkp wkgtqmksie ddpsitpgsv
     2101 vrsplslaai kdpdaifsss sktspppqqp tnpdtsipsl snstwtfnpp attpsaftrr
     2161 imfsakntwg esappptavt selwgapmsk vrgpppglss katgntsngw aglgtvgrss
     2221 sswglqsstn agwvstwlll knltpqidgs tlktlcmqhg pvqdfrlyln hgialtkyss
     2281 rdeaikaqga lnncvlgntt ifaespadse vhtllqqlsh ggqqqagata gagwslrpsn
     2341 ktgpppdtwg gsssqlwgap pssnslwsst gidsndqqra tpsslnsylp gdllggesm
//
LOCUS       XP_034185340            2399 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X9
            [Osmia lignaria lignaria].
ACCESSION   XP_034185340
VERSION     XP_034185340.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329449.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185340.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2399
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X9"
                     /calculated_mol_wt=263759
     Region          <1083..>1207
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1407..>1649
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1680..1714
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1750..1957
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          1966..2212
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2235..2304
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2399
                     /gene="gw"
                     /coded_by="XM_034329449.2:114..7313"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mfphnssshe istetnafvq nsmgdvvvlg knspigmgeg resenarycd iefinnsmma
       61 kptshleniv atdfltvlss qtgkfiltsr slicqsvass ttkhptknhl ltydnnniii
      121 ninnnnnnng ssgnnnnnnd ndnpklndrs nraehnhqdd rkptvhkttt ttttkrkhpg
      181 msgnkkpvss annsksmskt lsdnktsfsv lnirvlsisv nlrltgdkca vgegvpslvr
      241 ipksdrpssg hfsldsldny sllgsclhsg kynnvksnfs llsnnndnyk svillsstsn
      301 hyflrvemdg ganamklrth ldlfryfvks ffdglslqkq ynfillpeqd ddeynflind
      361 qmikskhrwi invqsingsr kfsltylelf sskilmeiii rcvkndafsc hnqdsnelcl
      421 srlctfvkyl ypigvdskie iflstylysk ieslrlvysd nlktlseins vfveillpav
      481 acsesnkhpm mksclirede tltnvvivvs vkyriskapl nfiiprnnvy vrdtssnees
      541 rlikegesva tsssitlivp gneltdqppr pfplrnapns aqsspswldl vaseltqrac
      601 vplslgckly vvkfsykvic dfsitwkgip shetsyerth ldvqflsisv qinettftyk
      661 ipnflrrtrt cllnddpleq gishdiflsl irkltattmn cenmnsmkde qlnvllfnsi
      721 rnlvecrmsi vgmenykicw sliitlrkll ndeiftsiri ltrnyvfllk ifrrmyysnv
      781 tlmneqqeqq qypndgcllk rtsdtlifkl kkktmemlss dinginrvat itvgalsfqd
      841 nvksnepsat ylasykysgs hasysisslr snlfkriklr cqafadvmnd kisredlcdl
      901 yrfkrrlykv hevigynven vftkslinck qvaligtttt tttpqtarfv ddsydcnknt
      961 spdfknkndt vrlrdrsqye msetvvrsgd dttseektnd knpiplrles netivngskr
     1021 iqliayarnl iaaslgvsdg tlqaqkndlk qtlkllfgsv ydmdqpnley kkrwgipgil
     1081 rlagggetsl nsagatnwgs tqtsttnnnn nnqsgwggss gnpsgnsgap gnwtgnsvnr
     1141 svaanqnpnq nqgpggqnvp gnvnkvnnpn qqsnqqsgpp tsqssgtaqg gqnsnqwsqg
     1201 ksnnpggpgq nssgqnnnss svqqqqqsns ssnnnqpnnq aqgsvnnnnt sasntpstkq
     1261 qleqlntmre alfsqdgwgc qhvnqdtnwd vptspepsmt kdgvpmwkpp vnngtdlwea
     1321 nlrnggqppp qqqaktpwgh tpatniggtw geddeaadss nmwtgaptss qpnsaagqwt
     1381 tstgnqsgmw agsnwgdpri dhrdprdlrs vdpremrdpr dhrmsldpre hmrvmdpmar
     1441 dpriadmrgd prgisgrlng anadamwgqp pgpphhqmgh qhpsgppakm lnpsninqwa
     1501 apppkdimpg kpsgweepsp ptqrrnvpny ddgtslwgnp aanqraipgs kvshwkdlps
     1561 nlgravgmqc ppgmpqnrmp gqpgmkpdvs gpmwghpgap ggrngswaeg shdagswdep
     1621 ktpstwneap lnpgtwgaps thkpkpmgpt gswvdtdmdp tpswghptkp tltkeviwns
     1681 refrylcdlg fkkedvelal rnremnreea lellsqlrpv dqwrrhdahs sydptnqatt
     1741 apayprfnhv aqqmsfppga gvpsgnttgs vggsvasasl lklqqqqqqa avplqqqqps
     1801 tnapqppfnq asrapqnqps tqqlrmlvqq iqlavqegyl nhqilnqpla pqtlillnql
     1861 lqqikvlqql hqqhsvqstm kgngqsvlqi svqitktkqq ianlqnqiav qqatymkqqq
     1921 qqqqqqqqqq qqhpappsqs seyykssvhd pmsalqnsft dltmnkeppv sqqqsrlnqw
     1981 klpsldkdge lvtnefsrap gttskpaatp agltrshssp nmnpllgqgd gtwstrlges
     2041 gwpdpgntds tdgkdwqptg aaagaaaftd lvpefepgkp wkgtqmksie ddpsitpgsv
     2101 vrsplslaai kdpdaifsss sktspppqqp tnpdtsipsl snstwtfnpp attpsaftrr
     2161 imfsakntwg esappptavt selwgapmsk vrgpppglss katgntsngw aglgtvgrss
     2221 sswglqsstn agwvstwlll knltpqidgs tlktlcmqhg pvqdfrlyln hgialtkyss
     2281 rdeaikaqga lnncvlgntt ifaespadse vhtllqqlsh ggqqqagata gagwslrpsn
     2341 ktgpppdtwg gsssqlwgap pssnslwsst gidsndqqra tpsslnsylp gdllggesm
//
LOCUS       XP_034185341            2399 aa            linear   INV 03-SEP-2025
DEFINITION  trinucleotide repeat containing adaptor protein gawky isoform X9
            [Osmia lignaria lignaria].
ACCESSION   XP_034185341
VERSION     XP_034185341.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329450.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185341.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2399
                     /product="trinucleotide repeat containing adaptor protein
                     gawky isoform X9"
                     /calculated_mol_wt=263759
     Region          <1083..>1207
                     /region_name="dermokine"
                     /note="cl42387"
                     /db_xref="CDD:455732"
     Region          1407..>1649
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          1680..1714
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          <1750..1957
                     /region_name="M_domain"
                     /note="M domain of GW182; pfam12938"
                     /db_xref="CDD:432890"
     Region          1966..2212
                     /region_name="TNRC6-PABC_bdg"
                     /note="TNRC6-PABC binding domain; pfam16608"
                     /db_xref="CDD:465195"
     Region          2235..2304
                     /region_name="RRM_GW182_like"
                     /note="RNA recognition motif (RRM) found in the GW182
                     family proteins; cd12435"
                     /db_xref="CDD:409869"
     CDS             1..2399
                     /gene="gw"
                     /coded_by="XM_034329450.2:257..7456"
                     /db_xref="GeneID:117606674"
ORIGIN      
        1 mfphnssshe istetnafvq nsmgdvvvlg knspigmgeg resenarycd iefinnsmma
       61 kptshleniv atdfltvlss qtgkfiltsr slicqsvass ttkhptknhl ltydnnniii
      121 ninnnnnnng ssgnnnnnnd ndnpklndrs nraehnhqdd rkptvhkttt ttttkrkhpg
      181 msgnkkpvss annsksmskt lsdnktsfsv lnirvlsisv nlrltgdkca vgegvpslvr
      241 ipksdrpssg hfsldsldny sllgsclhsg kynnvksnfs llsnnndnyk svillsstsn
      301 hyflrvemdg ganamklrth ldlfryfvks ffdglslqkq ynfillpeqd ddeynflind
      361 qmikskhrwi invqsingsr kfsltylelf sskilmeiii rcvkndafsc hnqdsnelcl
      421 srlctfvkyl ypigvdskie iflstylysk ieslrlvysd nlktlseins vfveillpav
      481 acsesnkhpm mksclirede tltnvvivvs vkyriskapl nfiiprnnvy vrdtssnees
      541 rlikegesva tsssitlivp gneltdqppr pfplrnapns aqsspswldl vaseltqrac
      601 vplslgckly vvkfsykvic dfsitwkgip shetsyerth ldvqflsisv qinettftyk
      661 ipnflrrtrt cllnddpleq gishdiflsl irkltattmn cenmnsmkde qlnvllfnsi
      721 rnlvecrmsi vgmenykicw sliitlrkll ndeiftsiri ltrnyvfllk ifrrmyysnv
      781 tlmneqqeqq qypndgcllk rtsdtlifkl kkktmemlss dinginrvat itvgalsfqd
      841 nvksnepsat ylasykysgs hasysisslr snlfkriklr cqafadvmnd kisredlcdl
      901 yrfkrrlykv hevigynven vftkslinck qvaligtttt tttpqtarfv ddsydcnknt
      961 spdfknkndt vrlrdrsqye msetvvrsgd dttseektnd knpiplrles netivngskr
     1021 iqliayarnl iaaslgvsdg tlqaqkndlk qtlkllfgsv ydmdqpnley kkrwgipgil
     1081 rlagggetsl nsagatnwgs tqtsttnnnn nnqsgwggss gnpsgnsgap gnwtgnsvnr
     1141 svaanqnpnq nqgpggqnvp gnvnkvnnpn qqsnqqsgpp tsqssgtaqg gqnsnqwsqg
     1201 ksnnpggpgq nssgqnnnss svqqqqqsns ssnnnqpnnq aqgsvnnnnt sasntpstkq
     1261 qleqlntmre alfsqdgwgc qhvnqdtnwd vptspepsmt kdgvpmwkpp vnngtdlwea
     1321 nlrnggqppp qqqaktpwgh tpatniggtw geddeaadss nmwtgaptss qpnsaagqwt
     1381 tstgnqsgmw agsnwgdpri dhrdprdlrs vdpremrdpr dhrmsldpre hmrvmdpmar
     1441 dpriadmrgd prgisgrlng anadamwgqp pgpphhqmgh qhpsgppakm lnpsninqwa
     1501 apppkdimpg kpsgweepsp ptqrrnvpny ddgtslwgnp aanqraipgs kvshwkdlps
     1561 nlgravgmqc ppgmpqnrmp gqpgmkpdvs gpmwghpgap ggrngswaeg shdagswdep
     1621 ktpstwneap lnpgtwgaps thkpkpmgpt gswvdtdmdp tpswghptkp tltkeviwns
     1681 refrylcdlg fkkedvelal rnremnreea lellsqlrpv dqwrrhdahs sydptnqatt
     1741 apayprfnhv aqqmsfppga gvpsgnttgs vggsvasasl lklqqqqqqa avplqqqqps
     1801 tnapqppfnq asrapqnqps tqqlrmlvqq iqlavqegyl nhqilnqpla pqtlillnql
     1861 lqqikvlqql hqqhsvqstm kgngqsvlqi svqitktkqq ianlqnqiav qqatymkqqq
     1921 qqqqqqqqqq qqhpappsqs seyykssvhd pmsalqnsft dltmnkeppv sqqqsrlnqw
     1981 klpsldkdge lvtnefsrap gttskpaatp agltrshssp nmnpllgqgd gtwstrlges
     2041 gwpdpgntds tdgkdwqptg aaagaaaftd lvpefepgkp wkgtqmksie ddpsitpgsv
     2101 vrsplslaai kdpdaifsss sktspppqqp tnpdtsipsl snstwtfnpp attpsaftrr
     2161 imfsakntwg esappptavt selwgapmsk vrgpppglss katgntsngw aglgtvgrss
     2221 sswglqsstn agwvstwlll knltpqidgs tlktlcmqhg pvqdfrlyln hgialtkyss
     2281 rdeaikaqga lnncvlgntt ifaespadse vhtllqqlsh ggqqqagata gagwslrpsn
     2341 ktgpppdtwg gsssqlwgap pssnslwsst gidsndqqra tpsslnsylp gdllggesm
//
LOCUS       XP_034184683            4613 aa            linear   INV 03-SEP-2025
DEFINITION  low-density lipoprotein receptor-related protein megalin [Osmia
            lignaria lignaria].
ACCESSION   XP_034184683
VERSION     XP_034184683.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328792.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184683.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4613
                     /product="low-density lipoprotein receptor-related protein
                     megalin"
                     /calculated_mol_wt=514369
     Region          60..91
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(65,73,84..85)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(77,80,84,90..91)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            87..91
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          100..139
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(105,118,129..130)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(122,125,129,135..136)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            132..136
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          144..181
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(149,160,171..172)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(164,167,171,177..178)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            174..178
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          189..224
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(194,203,214..215)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(207,210,214,220..221)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            217..221
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          229..263
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(234,242,253..254)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(246,249,253,259..260)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            256..260
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          268..305
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(273,287,298..299)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(291,294,298,304..305)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            301..305
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          349..385
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Site            order(349,352,365)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          471..>585
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          471..508
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          514..552
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          658..700
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          749..>887
                     /region_name="YvrE"
                     /note="Sugar lactone lactonase YvrE [Carbohydrate
                     transport and metabolism]; COG3386"
                     /db_xref="CDD:442613"
     Region          868..903
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1041..1070
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1041,1049,1060..1061)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1053,1056,1060,1066..1067)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1063..1067
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1074..1109
                     /region_name="Ldl_recept_a"
                     /note="Low-density lipoprotein receptor domain class A;
                     pfam00057"
                     /db_xref="CDD:395011"
     Site            order(1080,1088,1099..1100)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1092,1095,1099,1105..1106)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1102..1106
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1114..1147
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1119,1127,1138..1139)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1131,1134,1138,1144..1145)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1141..1145
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1154..1189
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1159,1168,1179..1180)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1172,1175,1179,1185..1186)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1182..1186
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1194..1229
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(1199,1208,1219..1220)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1212,1215,1219,1225..1226)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1222..1226
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1236..1270
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1243,1252,1263..1264)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1256,1259,1263,1269..1270)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1266..1270
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1279..1310
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1284,1292,1303..1304)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1296,1299,1303,1309..1310)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1306..1310
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1323..1357
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(1329,1339,1350..1351)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(1343,1346,1350,1356..1357)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            1353..1357
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          1410..1441
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          1513..1553
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1555..1599
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1578..1617
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          1601..1642
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          1886..1927
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2146..2192
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2196..2543
                     /region_name="Vgb"
                     /note="Streptogramin lyase [Defense mechanisms]; COG4257"
                     /db_xref="CDD:443399"
     Region          2214..2254
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          2239..2279
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          <2473..2618
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          2474..2504
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          2512..2552
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          2552..2594
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          2561..2593
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          2748..2782
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2753,2761,2772..2773)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2765,2768,2772,2778..2779)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2775..2779
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2787..2822
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2792,2800,2811..2812)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2804,2807,2811,2819..2820)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            order(2814..2815,2818..2820)
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2830..2863
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2836,2845,2856..2857)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2849,2852,2856,2862..2863)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2859..2863
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2915..2947
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(2921,2929,2940..2941)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2933,2936,2940,2946..2947)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2943..2947
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2961..2997
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2964,2978,2989..2990)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2982,2985,2989,2995..2996)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2992..2996
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3009..3040
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3014,3022,3033..3034)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3026,3029,3033,3039..3040)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3036..3040
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3051..3085
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3056,3064,3075..3076)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3068,3071,3075,3081..3082)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3078..3082
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3093..3126
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3100,3108,3119..3120)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3112,3115,3119,3125..3126)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3122..3126
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3143..3171
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <3168..3201
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          3284..3326
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3346..3389
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          3377..3414
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3414..3456
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     Region          3485..3535
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          3539..3572
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3544,3554,3565..3566)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3558,3561,3565,3571..3572)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3568..3572
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3581..3615
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3586,3594,3605..3606)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3598,3601,3605,3611..3612)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3608..3612
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3619..3652
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3624,3633,3644..3645)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3637,3640,3644,3650..3651)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3647..3651
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3660..3693
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3667,3675,3686..3687)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3679,3682,3686,3692..3693)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3689..3693
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3703..3742
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3708,3719,3730..3731)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3723,3726,3730,3736..3737)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3733..3737
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3744..3777
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3751,3759,3770..3771)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3763,3766,3770,3776..3777)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3773..3777
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3789..3823
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3794,3802,3813..3814)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3806,3809,3813,3819..3820)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3816..3820
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3828..3862
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3833,3841,3852..3853)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3845,3848,3852,3858..3859)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3855..3859
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3872..3903
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(3877,3885,3896..3897)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3889,3892,3896,3902..3903)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3899..3903
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3919..3947
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3919,3927,3938..3939)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3931,3934,3938,3944..3945)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3941..3945
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3958..3993
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(3964,3972,3983..3984)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(3976,3979,3983,3989..3990)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            3986..3990
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          3999..4035
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <4037..4074
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Region          4135..>4266
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          4167..4215
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          4216..4259
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          4226..4267
                     /region_name="Ldl_recept_b"
                     /note="Low-density lipoprotein receptor repeat class B;
                     pfam00058"
                     /db_xref="CDD:459654"
     Region          4250..4293
                     /region_name="LY"
                     /note="Low-density lipoprotein-receptor YWTD domain;
                     smart00135"
                     /db_xref="CDD:214531"
     CDS             1..4613
                     /gene="mgl"
                     /coded_by="XM_034328792.2:1..13842"
                     /db_xref="GeneID:117606410"
ORIGIN      
        1 mavaagprrp rtllvggnra sgntlgtktl ysvldgflli lalagiaqva airladgsgc
       61 gnnsyrcldg tcipatlvcn yqkdceaged efqscpppdc eagqitcgqy ifnktycipp
      121 hqrcdmtvdc vdgtdeagcn yrkcqpddfr cggttpelci pkekkcdgyl dcrngrdedk
      181 cgnnmkpacr ldqfrcnstq rcieqsarcn ykddcgdnsd eencnfqpct snqfrcanal
      241 cipksyhcdg ykdcqdgsde ksctmtscpg nkfmcpkgaa dgkplcidrs qlcdgkpdce
      301 detdeeaacs knmcnslrce yscraspmgg vcdcpagwvl andsrtcvdr decsewgfce
      361 qlcmntlgsy icscapgfvv qghkcralqh pdvklellva qeravwrmsa tgedrsivan
      421 ttgasgvdyl yssdllfwsd vktrkvhsep lngddndgsn vdiylasswg pvaiavdwig
      481 kklyvvdsiv qkidvfeldg ryhgialgsn ltnpmdiald ptvglmfvad skqvlranmd
      541 gtvafpvvse aaykvsgvav dviarrifwc dsqldyieta dyfgrnrvmv lrgphtpapt
      601 rltlfenkif wtdgtkqaim svdktegdys iqtiyklkda kaikalhplt qptvsnpcgn
      661 nnggcqhmci vtaasdqenr lgyrcacdig wrlssdqrnc nlvqeflmys qqrfikgrvl
      721 dpvmegfsda ilplvsrrar fvgldydahd qyiyysdvlq dviyrihqng tgreivlasq
      781 negveglavd waaknlyyid srkgtlnvls tqnvtyrrtl lknlkrprai vihpnhgyif
      841 fsewdrpani sraytdgsnl ivfrnltlgw pnglaidfak drlywcdall dhvqhsnlng
      901 tdvrtvnsrl irhpfsmaih eewmyitdwr ldaiirmhke tgeqevvlvr epdtnrlygv
      961 kvfsrdiqts ppdhpcsinn ggceklcfai pqnntnrygt srltrvcgcp ygerlqgner
     1021 scspdpeaep pleacpnkld ftcanqrcvs ytwvcdgend cldnsdeqnc tkptcgpnef
     1081 qcksgrcvpi sfrcdsendc gdysdevgcp niscsanqfa canfrcipst wkcdsendcg
     1141 dssdegdfcv aktcsfyqft cprsghcipq swvcdgdndc fdqqdemdcp pvtclstqft
     1201 cadqkmcvld sykcdgisdc ndgsdevgcp slapdqcntd kqfqcvssai cipkswycdg
     1261 tadcpdksde ptpcgqvscq pgyfkcrndk cvfkayicdg kddcgdgsde dtelhactap
     1321 efkcesqqwk cpnvtdrcin itsvcdgkfd cpngadegpr cdipeckyrg gfcsngciqt
     1381 plgkqctcpk gevlgkdgyd cedvnecdpp grcsqicant kggyycncvd gyslegdkhr
     1441 ckafnhsaaf liisnrhtil vadlqdqale rvpinvenvv atasnmhtgt ifwsdmklkk
     1501 isrldkgsdp qdviltgldl veglaydwig qnlywldskl ntievskeng asrmilvken
     1561 itqprgmcld pspgarwlfw tdwgenprie rigmdgtnrs tiintkiywp nglaldiank
     1621 riyfadskld fidtclydgs kriqvlassh yllhphsltl fedtmywtdr qlnrvlsahk
     1681 fygvnqtvvs hlisqplsih vhhpvlqpvt pnpcenasca hlcllspsvt egytckcqvg
     1741 fkllpngqcf eeetpylmvl rgsqivdvsl ipgsaktgyi tpvvgveggk lidydrkerm
     1801 iywlqtkddd dengtiystp ysggnrtefp npsgesgivg apsaiafdwl grnmfignky
     1861 asnieaikvd gkiryrtivl andgnktsva kpkaicvdpl eghifwvddg sfgiptkigr
     1921 vnmdgtnpmi lvnnverpea itidiptktv yfsslnpalv maistdgrnl irivseniel
     1981 pkalavhesk lylldprydk vervdlpdgg npttiidnds elktltiykk rvtgdhpcfv
     2041 knggceqicl pasggtrvca cgmeyrkvsd tacepfktfa vvtqldvarg yslkdakeam
     2101 vpisgighhi lhvdvhyagn wiywvefnrg iwngifrvrp ngtelqhvik qgigsngirg
     2161 laidwvaenl yftnvfphdn yvevckldgs nrkvlvktta dsprelavnp ikkylywidy
     2221 gqyprlgkay ldgsnwvpiv tsgistprdi tidpathdvy wvdskqdtiq kvsytggnre
     2281 iirrnlpnpm gvaiygndvy wvdrnlasvf kaskrvnisl pttvrsnlpk lrdivmfdan
     2341 sqppdptnpc sqgggsagcq qlcfampkes sapfkcecav gklaedgksc envdeylvfa
     2401 tkteiraigm dpentsvpfn svenltnvvg vdfdyqdkkl lftqirplar iawmradnps
     2461 sseihilisk ginpegisyd wtqkkvywtd ssnnsiyamd idgsnlvmia rvdrpraivv
     2521 dpcngslyft dwgrfgtsgk ifrttmagsl kkaiidkdls qpsglaidye drmlywtdav
     2581 rekierseld gknrqvlvsa tiypfaitvf rehiywtdlq lrgvyraekh tgankvelvk
     2641 rleesprdlh iysaarqqck vnpchisngg cdqschpgin gsaeckcddn srlvnedrmc
     2701 vpknvtcdss kfacrngrci srmwacdgdd dcgdgsdedv nycsyhscsp nefrcnngrc
     2761 ifktwkcdhe ndcrdgsdee gcvyppcgpg eftcanhrci pqsqvcngin dckdnvtsde
     2821 thercprntt cppnhlkcek tnicvepywl cdgdndcgdn sdenplhcaq rtcpqssfrc
     2881 pnhrcipatw ycdgdddcld gsdeppgycq segrtcfgdl ftcdngncip riyicdgdnd
     2941 cldhsdeder hqcndrkcde eteftctank vwnraqcipk kwlcdgdpdc vdgadenvtv
     3001 lhcptptpca enqftcdngr clnqnwlcdh dndcgdgsde gkfcnsrykp ctaqeftchn
     3061 fkcirkqfrc dgqddcgdhs dedgcpsksk nttcpnpgef mcgngncidq qlvcnkepdc
     3121 adesdepahc nvdecariem nqcahkcvdt ptsyycecnp gyrllengkt cedidecvet
     3181 pqvcsqqcqn tpgsfyckcn hywyeraade htckrrdnik pwiiftnkyy vrnmsidasn
     3241 yslmhqdlin vvaldadyse qmlyfcdvta ktifrapiag gereaiirhd slglegiavd
     3301 wvgrklywld rhakhldvae lngtnrktll tgiadpraiv vhpgtgylyf tswhlqayig
     3361 kmgmdgsnft riltweddia wpnaltidyf tdrifwadah ldyiafadle ghnrrkvltg
     3421 atvphvfait vfddfifwtd wnlkaisrae kfsganlrvl rntthrpydi hayhplrqlp
     3481 ynnpcmenng gcshlclisp passyllegy gqpgvtsykc kcpnqfvlek dgktcranct
     3541 dgqhrcgppd ekcipwywqc dgekdckdgs deptscpprv crpsvfqcan sncrpsvavc
     3601 dgaddcgdks deancgldcg elefkcksng rciheswkcd gdadckdgsd edpaichnrq
     3661 cdpnteftck ngrciqkvwm cdsdndcgdd sdepaymcrq rncttgwqrc pghanyrcip
     3721 kwlfcdgkdd crdgsdelpd ncpkcdpkmd fkcannrcvp kqwlcdfadd cgdgsdeaea
     3781 mckdryrecs esefrcdngk ciasrwrcds eddcgdnsde ngcqtfvckn ytfqcasghc
     3841 iasylrcdgt rdcrdmsdei gcpprypggr ycpqsrfqcd nnlcvyltdi cdgsddcgdg
     3901 sdenpnmcan fkcdttrrfq ctnhkciaky qvcdgidncg dgsdennmtm cfsrirpcdp
     3961 iteytcankk cvdraklcdf addcgdssde lgchhnkpcl esnkggcshy chnitdggyi
     4021 cacypgyiis qdnrkhcedv decstgqhqc sqictnlngt yscscrqgfq lsdnrsgvck
     4081 avetepmvlf angpeirayd vnekeelevi adekrvqavd fdpraeyvfw idghdnsirr
     4141 symvnakggq vkigyaqdln iksdslptsl avdwisgnly wteidssgat lfgrvmvsks
     4201 dgryrrslit iglelptsiv ldpehgrtiw adagsqpkie iawmdgdrrk qlvsdrigqp
     4261 ialaidysmn hvvywadrkl ntiesieqdg snrkvilkge nlhhpvsldv fesslywttr
     4321 rtgelmrqdk fgrgvpeviv kniaspggvk vyhplryntt lrnfchddqc thlcvpipgg
     4381 srclcsdsig plqqrettrs nerhcdatne rplpapricp crngglcqev ednklhcacp
     4441 ayfhgefcev ivgvrssnat aaivipivis vlvllgaaav imvlkkrpfg kpgglgsltg
     4501 aqsvsfrqgs nvefgvpahe rmepldveyn lgdvsnknrd fsnpmydavn tetgttngts
     4561 assmyelpae mkpssiqhke ppqlhlkrke ldptpvdsgk dtqqlveedk sec
//
LOCUS       XP_076545715            1582 aa            linear   INV 03-SEP-2025
DEFINITION  protein qui-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545715
VERSION     XP_076545715.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689600.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1582
                     /product="protein qui-1 isoform X1"
                     /calculated_mol_wt=175149
     Region          443..>526
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          811..847
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <818..1185
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          856..893
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          899..935
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          940..1017
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          985..1392
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1024..1059
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1066..1108
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1116..1153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1159..1194
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1199..1244
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1281..1316
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1322..1359
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1365..1405
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1582
                     /gene="LOC117606411"
                     /coded_by="XM_076689600.1:997..5745"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mnleveliag vikggpppkn lpaprlikif iagernefie erkqllelvg pelqsiyddm
       61 gievllvdmh yatnvnpdrn pflaehffde inaayrhsrg cflllligde ystgwlptrl
      121 teetygilst rysameeyye qdsncyvlka grydtvkdsw qnseptiret lkkaiesvvs
      181 eksdneeirn ilkstterql eyalkldetg egiiavtrsw kgkksskavs slkslvtsnl
      241 padniinvev eqktgginpd nrshkgylah lcrlvvnrvq tlvnasiead pemkskkkmv
      301 qeiyaeslmh lallrdvkac ednenierik qllidgksqk hgpimikggk ysgkssllsr
      361 iyadasswld satfkvvrlc astprsaysl ellrvlcqhv gflsgvldgn lpkdasfdpl
      421 ylnnwlgqii rrieeeplke qllillddlh rlhplecdiv aalswlpthl ppgvhllvtt
      481 avppemlrft pvqkerlrsn eilielqdst sqdsqettdn vfdtlerlig ecaanrigai
      541 lacteyglse tellemimpt ggdgplflqq gmfnfstwyl vrrtfneylk vrvlsgrllf
      601 swqypirdla rkryfsnqet lksyhgelan hfftedfedk deksspeeet pkketpfqsr
      661 prsqdtayni rhveeawlhl lragdveklk klavcafdfl laavqtisvs ylrcvlehsr
      721 ryllerdlel vyytvrkssd iltrdplqlg aqlicwlrpv aedggdlvsr mvtaamawcd
      781 gytapllvpl ngwlqpplpl qirtlscpqg vklveaapsg qhvivvpfqg daqlwhvmsg
      841 qlvhtfkdil ghsspiscla vtqqsqyllt gsedtsiivw dmkeftlklr iqehiapvlc
      901 ltsalknsvi lsggedsrii atslltgdvl ikvdhhrgpv nsilvdsage ilvsgssdct
      961 vclwclvrft llksivlpsa vtmldvsads vfllaacedq klylrslatg teihtlrghq
     1021 gevksiclar dcrraiagga kgkvsvfdmh sgrltrtlpa npsadvtavk vtekddflit
     1081 asgdrvtyws frgeeahakv qkstkqeslh phtapiscld isrdgamavt ggvdslvnlw
     1141 qlnthellst feghiasitc iafsasglfv asgsedktir vwgltlglvv atfkhqapvt
     1201 avtamfdgrr ivssdragti rvwaadtgtl iqsvcgpgrc ftvssdmrya icgtgdnqlr
     1261 ivslgagpee kyqvshsqdi tclvaspdsr llitgsrdms lkvwqlaggk lsqvlvghtd
     1321 hvtcvavsvl dksivvsgsk danlivwdin tgsdlhtlng hlgyitcvkl sgdgtlaasg
     1381 sedksliiwd tkkgcplnsi mlhvpvlgve latdlsrlsl hllehkcmpi lclyntpaqy
     1441 vklpdyvapw cdirdlrppg pkrpnkrllk kevsldsdtw rrkyghltsg vlrssvddgl
     1501 qrrfsvsasm deiskvglts spsglgaeqa alaqsqhfdq lealwnkqsp parprshgrt
     1561 lskqsslqat risdseeegv pd
//
LOCUS       XP_076545716            1582 aa            linear   INV 03-SEP-2025
DEFINITION  protein qui-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545716
VERSION     XP_076545716.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689601.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1582
                     /product="protein qui-1 isoform X1"
                     /calculated_mol_wt=175149
     Region          443..>526
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          811..847
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <818..1185
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          856..893
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          899..935
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          940..1017
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          985..1392
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1024..1059
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1066..1108
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1116..1153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1159..1194
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1199..1244
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1281..1316
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1322..1359
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1365..1405
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1582
                     /gene="LOC117606411"
                     /coded_by="XM_076689601.1:983..5731"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mnleveliag vikggpppkn lpaprlikif iagernefie erkqllelvg pelqsiyddm
       61 gievllvdmh yatnvnpdrn pflaehffde inaayrhsrg cflllligde ystgwlptrl
      121 teetygilst rysameeyye qdsncyvlka grydtvkdsw qnseptiret lkkaiesvvs
      181 eksdneeirn ilkstterql eyalkldetg egiiavtrsw kgkksskavs slkslvtsnl
      241 padniinvev eqktgginpd nrshkgylah lcrlvvnrvq tlvnasiead pemkskkkmv
      301 qeiyaeslmh lallrdvkac ednenierik qllidgksqk hgpimikggk ysgkssllsr
      361 iyadasswld satfkvvrlc astprsaysl ellrvlcqhv gflsgvldgn lpkdasfdpl
      421 ylnnwlgqii rrieeeplke qllillddlh rlhplecdiv aalswlpthl ppgvhllvtt
      481 avppemlrft pvqkerlrsn eilielqdst sqdsqettdn vfdtlerlig ecaanrigai
      541 lacteyglse tellemimpt ggdgplflqq gmfnfstwyl vrrtfneylk vrvlsgrllf
      601 swqypirdla rkryfsnqet lksyhgelan hfftedfedk deksspeeet pkketpfqsr
      661 prsqdtayni rhveeawlhl lragdveklk klavcafdfl laavqtisvs ylrcvlehsr
      721 ryllerdlel vyytvrkssd iltrdplqlg aqlicwlrpv aedggdlvsr mvtaamawcd
      781 gytapllvpl ngwlqpplpl qirtlscpqg vklveaapsg qhvivvpfqg daqlwhvmsg
      841 qlvhtfkdil ghsspiscla vtqqsqyllt gsedtsiivw dmkeftlklr iqehiapvlc
      901 ltsalknsvi lsggedsrii atslltgdvl ikvdhhrgpv nsilvdsage ilvsgssdct
      961 vclwclvrft llksivlpsa vtmldvsads vfllaacedq klylrslatg teihtlrghq
     1021 gevksiclar dcrraiagga kgkvsvfdmh sgrltrtlpa npsadvtavk vtekddflit
     1081 asgdrvtyws frgeeahakv qkstkqeslh phtapiscld isrdgamavt ggvdslvnlw
     1141 qlnthellst feghiasitc iafsasglfv asgsedktir vwgltlglvv atfkhqapvt
     1201 avtamfdgrr ivssdragti rvwaadtgtl iqsvcgpgrc ftvssdmrya icgtgdnqlr
     1261 ivslgagpee kyqvshsqdi tclvaspdsr llitgsrdms lkvwqlaggk lsqvlvghtd
     1321 hvtcvavsvl dksivvsgsk danlivwdin tgsdlhtlng hlgyitcvkl sgdgtlaasg
     1381 sedksliiwd tkkgcplnsi mlhvpvlgve latdlsrlsl hllehkcmpi lclyntpaqy
     1441 vklpdyvapw cdirdlrppg pkrpnkrllk kevsldsdtw rrkyghltsg vlrssvddgl
     1501 qrrfsvsasm deiskvglts spsglgaeqa alaqsqhfdq lealwnkqsp parprshgrt
     1561 lskqsslqat risdseeegv pd
//
LOCUS       XP_076545717            1582 aa            linear   INV 03-SEP-2025
DEFINITION  protein qui-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545717
VERSION     XP_076545717.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689602.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1582
                     /product="protein qui-1 isoform X1"
                     /calculated_mol_wt=175149
     Region          443..>526
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          811..847
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <818..1185
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          856..893
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          899..935
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          940..1017
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          985..1392
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1024..1059
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1066..1108
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1116..1153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1159..1194
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1199..1244
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1281..1316
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1322..1359
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1365..1405
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1582
                     /gene="LOC117606411"
                     /coded_by="XM_076689602.1:1037..5785"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mnleveliag vikggpppkn lpaprlikif iagernefie erkqllelvg pelqsiyddm
       61 gievllvdmh yatnvnpdrn pflaehffde inaayrhsrg cflllligde ystgwlptrl
      121 teetygilst rysameeyye qdsncyvlka grydtvkdsw qnseptiret lkkaiesvvs
      181 eksdneeirn ilkstterql eyalkldetg egiiavtrsw kgkksskavs slkslvtsnl
      241 padniinvev eqktgginpd nrshkgylah lcrlvvnrvq tlvnasiead pemkskkkmv
      301 qeiyaeslmh lallrdvkac ednenierik qllidgksqk hgpimikggk ysgkssllsr
      361 iyadasswld satfkvvrlc astprsaysl ellrvlcqhv gflsgvldgn lpkdasfdpl
      421 ylnnwlgqii rrieeeplke qllillddlh rlhplecdiv aalswlpthl ppgvhllvtt
      481 avppemlrft pvqkerlrsn eilielqdst sqdsqettdn vfdtlerlig ecaanrigai
      541 lacteyglse tellemimpt ggdgplflqq gmfnfstwyl vrrtfneylk vrvlsgrllf
      601 swqypirdla rkryfsnqet lksyhgelan hfftedfedk deksspeeet pkketpfqsr
      661 prsqdtayni rhveeawlhl lragdveklk klavcafdfl laavqtisvs ylrcvlehsr
      721 ryllerdlel vyytvrkssd iltrdplqlg aqlicwlrpv aedggdlvsr mvtaamawcd
      781 gytapllvpl ngwlqpplpl qirtlscpqg vklveaapsg qhvivvpfqg daqlwhvmsg
      841 qlvhtfkdil ghsspiscla vtqqsqyllt gsedtsiivw dmkeftlklr iqehiapvlc
      901 ltsalknsvi lsggedsrii atslltgdvl ikvdhhrgpv nsilvdsage ilvsgssdct
      961 vclwclvrft llksivlpsa vtmldvsads vfllaacedq klylrslatg teihtlrghq
     1021 gevksiclar dcrraiagga kgkvsvfdmh sgrltrtlpa npsadvtavk vtekddflit
     1081 asgdrvtyws frgeeahakv qkstkqeslh phtapiscld isrdgamavt ggvdslvnlw
     1141 qlnthellst feghiasitc iafsasglfv asgsedktir vwgltlglvv atfkhqapvt
     1201 avtamfdgrr ivssdragti rvwaadtgtl iqsvcgpgrc ftvssdmrya icgtgdnqlr
     1261 ivslgagpee kyqvshsqdi tclvaspdsr llitgsrdms lkvwqlaggk lsqvlvghtd
     1321 hvtcvavsvl dksivvsgsk danlivwdin tgsdlhtlng hlgyitcvkl sgdgtlaasg
     1381 sedksliiwd tkkgcplnsi mlhvpvlgve latdlsrlsl hllehkcmpi lclyntpaqy
     1441 vklpdyvapw cdirdlrppg pkrpnkrllk kevsldsdtw rrkyghltsg vlrssvddgl
     1501 qrrfsvsasm deiskvglts spsglgaeqa alaqsqhfdq lealwnkqsp parprshgrt
     1561 lskqsslqat risdseeegv pd
//
LOCUS       XP_076545718            1582 aa            linear   INV 03-SEP-2025
DEFINITION  protein qui-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545718
VERSION     XP_076545718.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689603.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1582
                     /product="protein qui-1 isoform X1"
                     /calculated_mol_wt=175149
     Region          443..>526
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          811..847
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <818..1185
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          856..893
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          899..935
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          940..1017
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          985..1392
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1024..1059
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1066..1108
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1116..1153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1159..1194
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1199..1244
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1281..1316
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1322..1359
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1365..1405
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1582
                     /gene="LOC117606411"
                     /coded_by="XM_076689603.1:1092..5840"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mnleveliag vikggpppkn lpaprlikif iagernefie erkqllelvg pelqsiyddm
       61 gievllvdmh yatnvnpdrn pflaehffde inaayrhsrg cflllligde ystgwlptrl
      121 teetygilst rysameeyye qdsncyvlka grydtvkdsw qnseptiret lkkaiesvvs
      181 eksdneeirn ilkstterql eyalkldetg egiiavtrsw kgkksskavs slkslvtsnl
      241 padniinvev eqktgginpd nrshkgylah lcrlvvnrvq tlvnasiead pemkskkkmv
      301 qeiyaeslmh lallrdvkac ednenierik qllidgksqk hgpimikggk ysgkssllsr
      361 iyadasswld satfkvvrlc astprsaysl ellrvlcqhv gflsgvldgn lpkdasfdpl
      421 ylnnwlgqii rrieeeplke qllillddlh rlhplecdiv aalswlpthl ppgvhllvtt
      481 avppemlrft pvqkerlrsn eilielqdst sqdsqettdn vfdtlerlig ecaanrigai
      541 lacteyglse tellemimpt ggdgplflqq gmfnfstwyl vrrtfneylk vrvlsgrllf
      601 swqypirdla rkryfsnqet lksyhgelan hfftedfedk deksspeeet pkketpfqsr
      661 prsqdtayni rhveeawlhl lragdveklk klavcafdfl laavqtisvs ylrcvlehsr
      721 ryllerdlel vyytvrkssd iltrdplqlg aqlicwlrpv aedggdlvsr mvtaamawcd
      781 gytapllvpl ngwlqpplpl qirtlscpqg vklveaapsg qhvivvpfqg daqlwhvmsg
      841 qlvhtfkdil ghsspiscla vtqqsqyllt gsedtsiivw dmkeftlklr iqehiapvlc
      901 ltsalknsvi lsggedsrii atslltgdvl ikvdhhrgpv nsilvdsage ilvsgssdct
      961 vclwclvrft llksivlpsa vtmldvsads vfllaacedq klylrslatg teihtlrghq
     1021 gevksiclar dcrraiagga kgkvsvfdmh sgrltrtlpa npsadvtavk vtekddflit
     1081 asgdrvtyws frgeeahakv qkstkqeslh phtapiscld isrdgamavt ggvdslvnlw
     1141 qlnthellst feghiasitc iafsasglfv asgsedktir vwgltlglvv atfkhqapvt
     1201 avtamfdgrr ivssdragti rvwaadtgtl iqsvcgpgrc ftvssdmrya icgtgdnqlr
     1261 ivslgagpee kyqvshsqdi tclvaspdsr llitgsrdms lkvwqlaggk lsqvlvghtd
     1321 hvtcvavsvl dksivvsgsk danlivwdin tgsdlhtlng hlgyitcvkl sgdgtlaasg
     1381 sedksliiwd tkkgcplnsi mlhvpvlgve latdlsrlsl hllehkcmpi lclyntpaqy
     1441 vklpdyvapw cdirdlrppg pkrpnkrllk kevsldsdtw rrkyghltsg vlrssvddgl
     1501 qrrfsvsasm deiskvglts spsglgaeqa alaqsqhfdq lealwnkqsp parprshgrt
     1561 lskqsslqat risdseeegv pd
//
LOCUS       XP_076545719            1582 aa            linear   INV 03-SEP-2025
DEFINITION  protein qui-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545719
VERSION     XP_076545719.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689604.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1582
                     /product="protein qui-1 isoform X1"
                     /calculated_mol_wt=175149
     Region          443..>526
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          811..847
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <818..1185
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          856..893
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          899..935
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          940..1017
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          985..1392
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1024..1059
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1066..1108
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1116..1153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1159..1194
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1199..1244
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1281..1316
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1322..1359
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1365..1405
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1582
                     /gene="LOC117606411"
                     /coded_by="XM_076689604.1:1100..5848"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mnleveliag vikggpppkn lpaprlikif iagernefie erkqllelvg pelqsiyddm
       61 gievllvdmh yatnvnpdrn pflaehffde inaayrhsrg cflllligde ystgwlptrl
      121 teetygilst rysameeyye qdsncyvlka grydtvkdsw qnseptiret lkkaiesvvs
      181 eksdneeirn ilkstterql eyalkldetg egiiavtrsw kgkksskavs slkslvtsnl
      241 padniinvev eqktgginpd nrshkgylah lcrlvvnrvq tlvnasiead pemkskkkmv
      301 qeiyaeslmh lallrdvkac ednenierik qllidgksqk hgpimikggk ysgkssllsr
      361 iyadasswld satfkvvrlc astprsaysl ellrvlcqhv gflsgvldgn lpkdasfdpl
      421 ylnnwlgqii rrieeeplke qllillddlh rlhplecdiv aalswlpthl ppgvhllvtt
      481 avppemlrft pvqkerlrsn eilielqdst sqdsqettdn vfdtlerlig ecaanrigai
      541 lacteyglse tellemimpt ggdgplflqq gmfnfstwyl vrrtfneylk vrvlsgrllf
      601 swqypirdla rkryfsnqet lksyhgelan hfftedfedk deksspeeet pkketpfqsr
      661 prsqdtayni rhveeawlhl lragdveklk klavcafdfl laavqtisvs ylrcvlehsr
      721 ryllerdlel vyytvrkssd iltrdplqlg aqlicwlrpv aedggdlvsr mvtaamawcd
      781 gytapllvpl ngwlqpplpl qirtlscpqg vklveaapsg qhvivvpfqg daqlwhvmsg
      841 qlvhtfkdil ghsspiscla vtqqsqyllt gsedtsiivw dmkeftlklr iqehiapvlc
      901 ltsalknsvi lsggedsrii atslltgdvl ikvdhhrgpv nsilvdsage ilvsgssdct
      961 vclwclvrft llksivlpsa vtmldvsads vfllaacedq klylrslatg teihtlrghq
     1021 gevksiclar dcrraiagga kgkvsvfdmh sgrltrtlpa npsadvtavk vtekddflit
     1081 asgdrvtyws frgeeahakv qkstkqeslh phtapiscld isrdgamavt ggvdslvnlw
     1141 qlnthellst feghiasitc iafsasglfv asgsedktir vwgltlglvv atfkhqapvt
     1201 avtamfdgrr ivssdragti rvwaadtgtl iqsvcgpgrc ftvssdmrya icgtgdnqlr
     1261 ivslgagpee kyqvshsqdi tclvaspdsr llitgsrdms lkvwqlaggk lsqvlvghtd
     1321 hvtcvavsvl dksivvsgsk danlivwdin tgsdlhtlng hlgyitcvkl sgdgtlaasg
     1381 sedksliiwd tkkgcplnsi mlhvpvlgve latdlsrlsl hllehkcmpi lclyntpaqy
     1441 vklpdyvapw cdirdlrppg pkrpnkrllk kevsldsdtw rrkyghltsg vlrssvddgl
     1501 qrrfsvsasm deiskvglts spsglgaeqa alaqsqhfdq lealwnkqsp parprshgrt
     1561 lskqsslqat risdseeegv pd
//
LOCUS       XP_076545720            1579 aa            linear   INV 03-SEP-2025
DEFINITION  protein qui-1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545720
VERSION     XP_076545720.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689605.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1579
                     /product="protein qui-1 isoform X2"
                     /calculated_mol_wt=174808
     Region          443..>526
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          <801..961
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          811..847
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          853..890
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          896..932
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          937..1014
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          982..1389
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1021..1056
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1063..1105
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1113..1150
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1156..1191
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1196..1241
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1278..1313
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1319..1356
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1362..1402
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1579
                     /gene="LOC117606411"
                     /coded_by="XM_076689605.1:997..5736"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mnleveliag vikggpppkn lpaprlikif iagernefie erkqllelvg pelqsiyddm
       61 gievllvdmh yatnvnpdrn pflaehffde inaayrhsrg cflllligde ystgwlptrl
      121 teetygilst rysameeyye qdsncyvlka grydtvkdsw qnseptiret lkkaiesvvs
      181 eksdneeirn ilkstterql eyalkldetg egiiavtrsw kgkksskavs slkslvtsnl
      241 padniinvev eqktgginpd nrshkgylah lcrlvvnrvq tlvnasiead pemkskkkmv
      301 qeiyaeslmh lallrdvkac ednenierik qllidgksqk hgpimikggk ysgkssllsr
      361 iyadasswld satfkvvrlc astprsaysl ellrvlcqhv gflsgvldgn lpkdasfdpl
      421 ylnnwlgqii rrieeeplke qllillddlh rlhplecdiv aalswlpthl ppgvhllvtt
      481 avppemlrft pvqkerlrsn eilielqdst sqdsqettdn vfdtlerlig ecaanrigai
      541 lacteyglse tellemimpt ggdgplflqq gmfnfstwyl vrrtfneylk vrvlsgrllf
      601 swqypirdla rkryfsnqet lksyhgelan hfftedfedk deksspeeet pkketpfqsr
      661 prsqdtayni rhveeawlhl lragdveklk klavcafdfl laavqtisvs ylrcvlehsr
      721 ryllerdlel vyytvrkssd iltrdplqlg aqlicwlrpv aedggdlvsr mvtaamawcd
      781 gytapllvpl ngwlqpplpl qirtlscpqg vklveaapsg qhvivvpfqg daqlwhvmsg
      841 qlvhtfkghs spisclavtq qsqylltgse dtsiivwdmk eftlklriqe hiapvlclts
      901 alknsvilsg gedsriiats lltgdvlikv dhhrgpvnsi lvdsageilv sgssdctvcl
      961 wclvrftllk sivlpsavtm ldvsadsvfl laacedqkly lrslatgtei htlrghqgev
     1021 ksiclardcr raiaggakgk vsvfdmhsgr ltrtlpanps advtavkvte kddflitasg
     1081 drvtywsfrg eeahakvqks tkqeslhpht apiscldisr dgamavtggv dslvnlwqln
     1141 thellstfeg hiasitciaf sasglfvasg sedktirvwg ltlglvvatf khqapvtavt
     1201 amfdgrrivs sdragtirvw aadtgtliqs vcgpgrcftv ssdmryaicg tgdnqlrivs
     1261 lgagpeekyq vshsqditcl vaspdsrlli tgsrdmslkv wqlaggklsq vlvghtdhvt
     1321 cvavsvldks ivvsgskdan livwdintgs dlhtlnghlg yitcvklsgd gtlaasgsed
     1381 ksliiwdtkk gcplnsimlh vpvlgvelat dlsrlslhll ehkcmpilcl yntpaqyvkl
     1441 pdyvapwcdi rdlrppgpkr pnkrllkkev sldsdtwrrk yghltsgvlr ssvddglqrr
     1501 fsvsasmdei skvgltssps glgaeqaala qsqhfdqlea lwnkqsppar prshgrtlsk
     1561 qsslqatris dseeegvpd
//
LOCUS       XP_076545721            1523 aa            linear   INV 03-SEP-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545721
VERSION     XP_076545721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689606.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1523
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X3"
                     /calculated_mol_wt=168555
     Region          384..>467
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          752..788
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <759..1126
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          797..834
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          840..876
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          881..958
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          926..1333
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          965..1000
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1007..1049
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1057..1094
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1100..1135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1140..1185
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1222..1257
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1263..1300
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1306..1346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1523
                     /gene="LOC117606411"
                     /coded_by="XM_076689606.1:1001..5572"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mgievllvdm hyatnvnpdr npflaehffd einaayrhsr gcflllligd eystgwlptr
       61 lteetygils trysameeyy eqdsncyvlk agrydtvkds wqnseptire tlkkaiesvv
      121 seksdneeir nilkstterq leyalkldet gegiiavtrs wkgkksskav sslkslvtsn
      181 lpadniinve veqktgginp dnrshkgyla hlcrlvvnrv qtlvnasiea dpemkskkkm
      241 vqeiyaeslm hlallrdvka cednenieri kqllidgksq khgpimikgg kysgksslls
      301 riyadasswl dsatfkvvrl castprsays lellrvlcqh vgflsgvldg nlpkdasfdp
      361 lylnnwlgqi irrieeeplk eqllillddl hrlhplecdi vaalswlpth lppgvhllvt
      421 tavppemlrf tpvqkerlrs neilielqds tsqdsqettd nvfdtlerli gecaanriga
      481 ilacteygls etellemimp tggdgplflq qgmfnfstwy lvrrtfneyl kvrvlsgrll
      541 fswqypirdl arkryfsnqe tlksyhgela nhfftedfed kdeksspeee tpkketpfqs
      601 rprsqdtayn irhveeawlh llragdvekl kklavcafdf llaavqtisv sylrcvlehs
      661 rryllerdle lvyytvrkss diltrdplql gaqlicwlrp vaedggdlvs rmvtaamawc
      721 dgytapllvp lngwlqpplp lqirtlscpq gvklveaaps gqhvivvpfq gdaqlwhvms
      781 gqlvhtfkdi lghsspiscl avtqqsqyll tgsedtsiiv wdmkeftlkl riqehiapvl
      841 cltsalknsv ilsggedsri iatslltgdv likvdhhrgp vnsilvdsag eilvsgssdc
      901 tvclwclvrf tllksivlps avtmldvsad svfllaaced qklylrslat gteihtlrgh
      961 qgevksicla rdcrraiagg akgkvsvfdm hsgrltrtlp anpsadvtav kvtekddfli
     1021 tasgdrvtyw sfrgeeahak vqkstkqesl hphtapiscl disrdgamav tggvdslvnl
     1081 wqlnthells tfeghiasit ciafsasglf vasgsedkti rvwgltlglv vatfkhqapv
     1141 tavtamfdgr rivssdragt irvwaadtgt liqsvcgpgr cftvssdmry aicgtgdnql
     1201 rivslgagpe ekyqvshsqd itclvaspds rllitgsrdm slkvwqlagg klsqvlvght
     1261 dhvtcvavsv ldksivvsgs kdanlivwdi ntgsdlhtln ghlgyitcvk lsgdgtlaas
     1321 gsedksliiw dtkkgcplns imlhvpvlgv elatdlsrls lhllehkcmp ilclyntpaq
     1381 yvklpdyvap wcdirdlrpp gpkrpnkrll kkevsldsdt wrrkyghlts gvlrssvddg
     1441 lqrrfsvsas mdeiskvglt sspsglgaeq aalaqsqhfd qlealwnkqs pparprshgr
     1501 tlskqsslqa trisdseeeg vpd
//
LOCUS       XP_076545722            1523 aa            linear   INV 03-SEP-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545722
VERSION     XP_076545722.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689607.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1523
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X3"
                     /calculated_mol_wt=168555
     Region          384..>467
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          752..788
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <759..1126
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          797..834
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          840..876
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          881..958
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          926..1333
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          965..1000
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1007..1049
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1057..1094
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1100..1135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1140..1185
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1222..1257
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1263..1300
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1306..1346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1523
                     /gene="LOC117606411"
                     /coded_by="XM_076689607.1:398..4969"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mgievllvdm hyatnvnpdr npflaehffd einaayrhsr gcflllligd eystgwlptr
       61 lteetygils trysameeyy eqdsncyvlk agrydtvkds wqnseptire tlkkaiesvv
      121 seksdneeir nilkstterq leyalkldet gegiiavtrs wkgkksskav sslkslvtsn
      181 lpadniinve veqktgginp dnrshkgyla hlcrlvvnrv qtlvnasiea dpemkskkkm
      241 vqeiyaeslm hlallrdvka cednenieri kqllidgksq khgpimikgg kysgksslls
      301 riyadasswl dsatfkvvrl castprsays lellrvlcqh vgflsgvldg nlpkdasfdp
      361 lylnnwlgqi irrieeeplk eqllillddl hrlhplecdi vaalswlpth lppgvhllvt
      421 tavppemlrf tpvqkerlrs neilielqds tsqdsqettd nvfdtlerli gecaanriga
      481 ilacteygls etellemimp tggdgplflq qgmfnfstwy lvrrtfneyl kvrvlsgrll
      541 fswqypirdl arkryfsnqe tlksyhgela nhfftedfed kdeksspeee tpkketpfqs
      601 rprsqdtayn irhveeawlh llragdvekl kklavcafdf llaavqtisv sylrcvlehs
      661 rryllerdle lvyytvrkss diltrdplql gaqlicwlrp vaedggdlvs rmvtaamawc
      721 dgytapllvp lngwlqpplp lqirtlscpq gvklveaaps gqhvivvpfq gdaqlwhvms
      781 gqlvhtfkdi lghsspiscl avtqqsqyll tgsedtsiiv wdmkeftlkl riqehiapvl
      841 cltsalknsv ilsggedsri iatslltgdv likvdhhrgp vnsilvdsag eilvsgssdc
      901 tvclwclvrf tllksivlps avtmldvsad svfllaaced qklylrslat gteihtlrgh
      961 qgevksicla rdcrraiagg akgkvsvfdm hsgrltrtlp anpsadvtav kvtekddfli
     1021 tasgdrvtyw sfrgeeahak vqkstkqesl hphtapiscl disrdgamav tggvdslvnl
     1081 wqlnthells tfeghiasit ciafsasglf vasgsedkti rvwgltlglv vatfkhqapv
     1141 tavtamfdgr rivssdragt irvwaadtgt liqsvcgpgr cftvssdmry aicgtgdnql
     1201 rivslgagpe ekyqvshsqd itclvaspds rllitgsrdm slkvwqlagg klsqvlvght
     1261 dhvtcvavsv ldksivvsgs kdanlivwdi ntgsdlhtln ghlgyitcvk lsgdgtlaas
     1321 gsedksliiw dtkkgcplns imlhvpvlgv elatdlsrls lhllehkcmp ilclyntpaq
     1381 yvklpdyvap wcdirdlrpp gpkrpnkrll kkevsldsdt wrrkyghlts gvlrssvddg
     1441 lqrrfsvsas mdeiskvglt sspsglgaeq aalaqsqhfd qlealwnkqs pparprshgr
     1501 tlskqsslqa trisdseeeg vpd
//
LOCUS       XP_076545723            1523 aa            linear   INV 03-SEP-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545723
VERSION     XP_076545723.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689608.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1523
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X3"
                     /calculated_mol_wt=168555
     Region          384..>467
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          752..788
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <759..1126
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          797..834
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          840..876
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          881..958
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          926..1333
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          965..1000
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1007..1049
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1057..1094
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1100..1135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1140..1185
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1222..1257
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1263..1300
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1306..1346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1523
                     /gene="LOC117606411"
                     /coded_by="XM_076689608.1:421..4992"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mgievllvdm hyatnvnpdr npflaehffd einaayrhsr gcflllligd eystgwlptr
       61 lteetygils trysameeyy eqdsncyvlk agrydtvkds wqnseptire tlkkaiesvv
      121 seksdneeir nilkstterq leyalkldet gegiiavtrs wkgkksskav sslkslvtsn
      181 lpadniinve veqktgginp dnrshkgyla hlcrlvvnrv qtlvnasiea dpemkskkkm
      241 vqeiyaeslm hlallrdvka cednenieri kqllidgksq khgpimikgg kysgksslls
      301 riyadasswl dsatfkvvrl castprsays lellrvlcqh vgflsgvldg nlpkdasfdp
      361 lylnnwlgqi irrieeeplk eqllillddl hrlhplecdi vaalswlpth lppgvhllvt
      421 tavppemlrf tpvqkerlrs neilielqds tsqdsqettd nvfdtlerli gecaanriga
      481 ilacteygls etellemimp tggdgplflq qgmfnfstwy lvrrtfneyl kvrvlsgrll
      541 fswqypirdl arkryfsnqe tlksyhgela nhfftedfed kdeksspeee tpkketpfqs
      601 rprsqdtayn irhveeawlh llragdvekl kklavcafdf llaavqtisv sylrcvlehs
      661 rryllerdle lvyytvrkss diltrdplql gaqlicwlrp vaedggdlvs rmvtaamawc
      721 dgytapllvp lngwlqpplp lqirtlscpq gvklveaaps gqhvivvpfq gdaqlwhvms
      781 gqlvhtfkdi lghsspiscl avtqqsqyll tgsedtsiiv wdmkeftlkl riqehiapvl
      841 cltsalknsv ilsggedsri iatslltgdv likvdhhrgp vnsilvdsag eilvsgssdc
      901 tvclwclvrf tllksivlps avtmldvsad svfllaaced qklylrslat gteihtlrgh
      961 qgevksicla rdcrraiagg akgkvsvfdm hsgrltrtlp anpsadvtav kvtekddfli
     1021 tasgdrvtyw sfrgeeahak vqkstkqesl hphtapiscl disrdgamav tggvdslvnl
     1081 wqlnthells tfeghiasit ciafsasglf vasgsedkti rvwgltlglv vatfkhqapv
     1141 tavtamfdgr rivssdragt irvwaadtgt liqsvcgpgr cftvssdmry aicgtgdnql
     1201 rivslgagpe ekyqvshsqd itclvaspds rllitgsrdm slkvwqlagg klsqvlvght
     1261 dhvtcvavsv ldksivvsgs kdanlivwdi ntgsdlhtln ghlgyitcvk lsgdgtlaas
     1321 gsedksliiw dtkkgcplns imlhvpvlgv elatdlsrls lhllehkcmp ilclyntpaq
     1381 yvklpdyvap wcdirdlrpp gpkrpnkrll kkevsldsdt wrrkyghlts gvlrssvddg
     1441 lqrrfsvsas mdeiskvglt sspsglgaeq aalaqsqhfd qlealwnkqs pparprshgr
     1501 tlskqsslqa trisdseeeg vpd
//
LOCUS       XP_076545724            1523 aa            linear   INV 03-SEP-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545724
VERSION     XP_076545724.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689609.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1523
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X3"
                     /calculated_mol_wt=168555
     Region          384..>467
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          752..788
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <759..1126
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          797..834
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          840..876
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          881..958
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          926..1333
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          965..1000
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1007..1049
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1057..1094
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1100..1135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1140..1185
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1222..1257
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1263..1300
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1306..1346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1523
                     /gene="LOC117606411"
                     /coded_by="XM_076689609.1:246..4817"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mgievllvdm hyatnvnpdr npflaehffd einaayrhsr gcflllligd eystgwlptr
       61 lteetygils trysameeyy eqdsncyvlk agrydtvkds wqnseptire tlkkaiesvv
      121 seksdneeir nilkstterq leyalkldet gegiiavtrs wkgkksskav sslkslvtsn
      181 lpadniinve veqktgginp dnrshkgyla hlcrlvvnrv qtlvnasiea dpemkskkkm
      241 vqeiyaeslm hlallrdvka cednenieri kqllidgksq khgpimikgg kysgksslls
      301 riyadasswl dsatfkvvrl castprsays lellrvlcqh vgflsgvldg nlpkdasfdp
      361 lylnnwlgqi irrieeeplk eqllillddl hrlhplecdi vaalswlpth lppgvhllvt
      421 tavppemlrf tpvqkerlrs neilielqds tsqdsqettd nvfdtlerli gecaanriga
      481 ilacteygls etellemimp tggdgplflq qgmfnfstwy lvrrtfneyl kvrvlsgrll
      541 fswqypirdl arkryfsnqe tlksyhgela nhfftedfed kdeksspeee tpkketpfqs
      601 rprsqdtayn irhveeawlh llragdvekl kklavcafdf llaavqtisv sylrcvlehs
      661 rryllerdle lvyytvrkss diltrdplql gaqlicwlrp vaedggdlvs rmvtaamawc
      721 dgytapllvp lngwlqpplp lqirtlscpq gvklveaaps gqhvivvpfq gdaqlwhvms
      781 gqlvhtfkdi lghsspiscl avtqqsqyll tgsedtsiiv wdmkeftlkl riqehiapvl
      841 cltsalknsv ilsggedsri iatslltgdv likvdhhrgp vnsilvdsag eilvsgssdc
      901 tvclwclvrf tllksivlps avtmldvsad svfllaaced qklylrslat gteihtlrgh
      961 qgevksicla rdcrraiagg akgkvsvfdm hsgrltrtlp anpsadvtav kvtekddfli
     1021 tasgdrvtyw sfrgeeahak vqkstkqesl hphtapiscl disrdgamav tggvdslvnl
     1081 wqlnthells tfeghiasit ciafsasglf vasgsedkti rvwgltlglv vatfkhqapv
     1141 tavtamfdgr rivssdragt irvwaadtgt liqsvcgpgr cftvssdmry aicgtgdnql
     1201 rivslgagpe ekyqvshsqd itclvaspds rllitgsrdm slkvwqlagg klsqvlvght
     1261 dhvtcvavsv ldksivvsgs kdanlivwdi ntgsdlhtln ghlgyitcvk lsgdgtlaas
     1321 gsedksliiw dtkkgcplns imlhvpvlgv elatdlsrls lhllehkcmp ilclyntpaq
     1381 yvklpdyvap wcdirdlrpp gpkrpnkrll kkevsldsdt wrrkyghlts gvlrssvddg
     1441 lqrrfsvsas mdeiskvglt sspsglgaeq aalaqsqhfd qlealwnkqs pparprshgr
     1501 tlskqsslqa trisdseeeg vpd
//
LOCUS       XP_076545725            1523 aa            linear   INV 03-SEP-2025
DEFINITION  NACHT domain- and WD repeat-containing protein 1 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545725
VERSION     XP_076545725.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689610.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1523
                     /product="NACHT domain- and WD repeat-containing protein 1
                     isoform X3"
                     /calculated_mol_wt=168555
     Region          384..>467
                     /region_name="MalT"
                     /note="ATP-, maltotriose- and DNA-dependent
                     transcriptional regulator MalT [Transcription]; COG2909"
                     /db_xref="CDD:442153"
     Region          752..788
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <759..1126
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          797..834
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          840..876
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          881..958
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          926..1333
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          965..1000
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1007..1049
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1057..1094
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1100..1135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1140..1185
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1222..1257
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1263..1300
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1306..1346
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1523
                     /gene="LOC117606411"
                     /coded_by="XM_076689610.1:94..4665"
                     /db_xref="GeneID:117606411"
ORIGIN      
        1 mgievllvdm hyatnvnpdr npflaehffd einaayrhsr gcflllligd eystgwlptr
       61 lteetygils trysameeyy eqdsncyvlk agrydtvkds wqnseptire tlkkaiesvv
      121 seksdneeir nilkstterq leyalkldet gegiiavtrs wkgkksskav sslkslvtsn
      181 lpadniinve veqktgginp dnrshkgyla hlcrlvvnrv qtlvnasiea dpemkskkkm
      241 vqeiyaeslm hlallrdvka cednenieri kqllidgksq khgpimikgg kysgksslls
      301 riyadasswl dsatfkvvrl castprsays lellrvlcqh vgflsgvldg nlpkdasfdp
      361 lylnnwlgqi irrieeeplk eqllillddl hrlhplecdi vaalswlpth lppgvhllvt
      421 tavppemlrf tpvqkerlrs neilielqds tsqdsqettd nvfdtlerli gecaanriga
      481 ilacteygls etellemimp tggdgplflq qgmfnfstwy lvrrtfneyl kvrvlsgrll
      541 fswqypirdl arkryfsnqe tlksyhgela nhfftedfed kdeksspeee tpkketpfqs
      601 rprsqdtayn irhveeawlh llragdvekl kklavcafdf llaavqtisv sylrcvlehs
      661 rryllerdle lvyytvrkss diltrdplql gaqlicwlrp vaedggdlvs rmvtaamawc
      721 dgytapllvp lngwlqpplp lqirtlscpq gvklveaaps gqhvivvpfq gdaqlwhvms
      781 gqlvhtfkdi lghsspiscl avtqqsqyll tgsedtsiiv wdmkeftlkl riqehiapvl
      841 cltsalknsv ilsggedsri iatslltgdv likvdhhrgp vnsilvdsag eilvsgssdc
      901 tvclwclvrf tllksivlps avtmldvsad svfllaaced qklylrslat gteihtlrgh
      961 qgevksicla rdcrraiagg akgkvsvfdm hsgrltrtlp anpsadvtav kvtekddfli
     1021 tasgdrvtyw sfrgeeahak vqkstkqesl hphtapiscl disrdgamav tggvdslvnl
     1081 wqlnthells tfeghiasit ciafsasglf vasgsedkti rvwgltlglv vatfkhqapv
     1141 tavtamfdgr rivssdragt irvwaadtgt liqsvcgpgr cftvssdmry aicgtgdnql
     1201 rivslgagpe ekyqvshsqd itclvaspds rllitgsrdm slkvwqlagg klsqvlvght
     1261 dhvtcvavsv ldksivvsgs kdanlivwdi ntgsdlhtln ghlgyitcvk lsgdgtlaas
     1321 gsedksliiw dtkkgcplns imlhvpvlgv elatdlsrls lhllehkcmp ilclyntpaq
     1381 yvklpdyvap wcdirdlrpp gpkrpnkrll kkevsldsdt wrrkyghlts gvlrssvddg
     1441 lqrrfsvsas mdeiskvglt sspsglgaeq aalaqsqhfd qlealwnkqs pparprshgr
     1501 tlskqsslqa trisdseeeg vpd
//
LOCUS       XP_034184689            1565 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606412 [Osmia lignaria lignaria].
ACCESSION   XP_034184689
VERSION     XP_034184689.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328798.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184689.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1565
                     /product="uncharacterized protein LOC117606412"
                     /calculated_mol_wt=178512
     CDS             1..1565
                     /gene="LOC117606412"
                     /coded_by="XM_034328798.2:182..4879"
                     /db_xref="GeneID:117606412"
ORIGIN      
        1 mlklllflml inaieyhayt qenyvlnaal khtsnvfkkl klnegtedln envilrinqt
       61 vvehvrntsk iilsrrkrel sedfnkgint lngfkdfiql nfrnvrdvni fslkngyevi
      121 wfaaildgfg vsllqinkds lfhvtaypvt ngtrlivnec nygtlliiqn qdnsihvlrl
      181 skvndkyylh liqdlesndi shltiwngmn qlhlgissqs stsiftwfgd yfdlvqvinl
      241 ntkklvpfqn kgfmylaatg svtlifkhfl ksdeflviqk lpaskdasff qlreghftdn
      301 ylslstessv viykdihgrf vpfqqipsgk ptvpivsnka ilfftlqedt lltyqyngwr
      361 fieldvklhg vhrlqtvnly gkqlllmkyk ndtwtlkqli wtkrktykdi qeeikawnik
      421 akniaqrqln eisnlnnsvq ilnghidqlf vhnvnqhnlk alknvsvqyn kvssklqeqs
      481 svinnkwhsg nvtvtslsak kiqmrckttc rinkvnikgn tdlllklkvl pnknevssfk
      541 tlkvkeiknw kcpifnllid nivvsksing mllenlhknt lkitgnqtil grhsfisinv
      601 tntvmplnia syfakkeiqa kkvkvkeltl teggillpln gsstvitgsi sasritvkdk
      661 irlqgsvtgs waknfspiky ipdsisiyhd vnienarien lqstdlinnk tgsvkdilsn
      721 aiilhenvpv slvfssqktk wnnitihgpq nwvtansqnv itgkkhmlhn veitkfsyen
      781 lrfprietal cgasiivpei katvltvnnv tvksltsphv fgnladksgf ifkplnssae
      841 ryyyyynvka kniftshtsn inltskaler ftnswvepni lkssieatdl tinvlqssmk
      901 fypkwsrvir nvvskkniyv gninkinftd flvkaikled iislrnvtfn ngfttnhihi
      961 sypllpliqt dkdlilhkkr isrnievnvi nlpylfglek nhtsaniiik gsaifptepn
     1021 ihninknnlk dlfmrtwiit tattfvgknl hvtdvvlkgn itinnstnil yreawknise
     1081 rvlsktkvqt iivdaslntv etssvvgsnt stivssvsdf nymfnnslmk graqdvkakw
     1141 tfdkliilgk mnarkkinnm nlktdvmryd srrnivtgkk tvlilttknl nglnfaewad
     1201 salipkenrv iikgqkhflt aafnditlsg timghsvdea llqfrnqtih gqkiihgsir
     1261 vsrifidglv ndvnltnlid hqlkkqsslq kiettielqk kliitgnliv ndtygganmk
     1321 nfeksysyip fitdksnnys kiaetinlal rnhanyihkl eivknitsis rqgtimqreq
     1381 ckvrnfskyc vsehienivf rsdtknsili ksifleetef vvwikfhfvs iykynnvnkn
     1441 lirvkdlhip riidafveqm fnslwiilrl tsqtlvlhyq pwqelqeyvl patdvfalsr
     1501 spnnqlllll ssgvwnlkgl aspqniieip lkgqvqtfvn gfdyfvkyts snntiltkvr
     1561 yvgnc
//
LOCUS       XP_034184696            1061 aa            linear   INV 03-SEP-2025
DEFINITION  protein transport protein Sec24AB [Osmia lignaria lignaria].
ACCESSION   XP_034184696
VERSION     XP_034184696.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328805.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184696.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1061
                     /product="protein transport protein Sec24AB"
                     /calculated_mol_wt=118720
     Region          232..1061
                     /region_name="COG5028"
                     /note="Vesicle coat complex COPII, subunit SEC24/subunit
                     SFB2/subunit SFB3 [Intracellular trafficking and
                     secretion]"
                     /db_xref="CDD:227361"
     CDS             1..1061
                     /gene="Sec24AB"
                     /coded_by="XM_034328805.2:186..3371"
                     /db_xref="GeneID:117606415"
ORIGIN      
        1 msqptdyqyr pygnggnlks gppqmvngqa sathhngmqp gnynftgtss shssrdpsre
       61 tsrdpsptsy lhnqyksplq rplvppmngq qnsmnplmpm pqpppppppl ppppplppsa
      121 qqapvrlsqn qqyppmynvq nqfqvpvtkh qapprdelrn lqgnsqidps ntesklyktd
      181 sqstvqgaqt fvqqqqeqni ipttmqqprg qmsyqqnlgg srmypkntyg iqhatsvpnt
      241 mpyrtvgsvd tqakipaapi gfpgqrmyps qppnpvnipl dsmnkrypes ypeqmnhlnn
      301 qmnmmsvaqa gynklwgmet vdllqcrnvl paekveppki klhqefldsv ncspdifrct
      361 ltkipesnsl lqksrlplgv lihpfkdlnh lpviqcstiv rcracrtyin pfvyfndskr
      421 wkcnlcyrvn elpdefqfdp vtksygdpsr rpevntstie fiapseymlr ppqpavylfv
      481 ldvsrlaves gylsivcnvi seelsklpgd srtqigflav dsaihffgip dnvsqpqemi
      541 mldiddgflp cpdnlivnlk ereelvrdll aqlptkfqgt hdtssalgaa lqaayklmsp
      601 tggrvtvfqt clpnlgpgll qaredpnsrs skdvphlnpv tdfykrlald csgqqiavdl
      661 fllnsqycdl atlsgmckfs ggciyhlplf rgsksqnaet ldrilrrylt rkigfeavmr
      721 irctrglsih tfhgnffvrs tdllslpnvn pdagfgmqis ieenlsdiqn vcfqaallyt
      781 sskgerrirv htlclpvvst lsdvlhsadq qciagllskm avdrsqqssl sdardalinv
      841 aidvlsaykl sqstapgglv apaslkllpl yiiallkcva frsgtntrld drvfamcqlk
      901 tlplfqliqt iypdlypiha lddrnlkdid gklcpqpprl hlsaekldsr gaflmdagdr
      961 ifiligknih psfccnilgv safasipeel yelpeietae serlrnfvfs lqeekpypat
     1021 vqiirddshf rtlfierlve drfenslsyy eflqhlktqv k
//
LOCUS       XP_034184699             977 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606417 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184699
VERSION     XP_034184699.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328808.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184699.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..977
                     /product="uncharacterized protein LOC117606417 isoform X1"
                     /calculated_mol_wt=110449
     CDS             1..977
                     /gene="LOC117606417"
                     /coded_by="XM_034328808.2:1334..4267"
                     /db_xref="GeneID:117606417"
ORIGIN      
        1 mklnyivavl iwirtvtatl esdsrgnwsn ekelnyeqer ignqtkeeit gdvhspheri
       61 filssestes ikaerpeeld vkfanknedn hevnkqlakq lvqpymlplp letryfqqdt
      121 hnesrknvsr lartsqnspn etatveyips keilvqdstt tsnnypiqdk fndlaysnse
      181 dqadfeyvge fgqrvrssps frpksnenvg rdrfrpldtl slsedyrkhe rknsrpfvik
      241 klnasdsian lyskdrhpvv asgkasefqr gpkpkktgge eeeeysdtid ynkenvvsss
      301 skdttsppvq qvsfgkftgp ivvadlpqqk rysfatttgy tiindqpqsi pasleiiksk
      361 aaessylats savlnplqvg vammnvgqdi nsvndpvtft keylrnepdp sqlpvttdie
      421 siiqndaaid sgnsslqtdq lsqqeeiskv vasngptqsv eiqksvevfh tapvheihyp
      481 lefvpyvqqs snfkqrqhtd yrkpqggqek dqrpnvyknn eildetvssn npersryeyt
      541 vneddvvqvd qtvahstdvk pvfdareqle dvqdnqtagk yskiqripdt iategiakne
      601 hdpvkgvpll ntqpvgiidn lpalgnienp qasqqvpeil likptnepst evrflmavpq
      661 hypgekvhvh rtvnvdekkv pyhiekviek qitipqpfpv qvpvdrvvek qiripypvhv
      721 ekviekkvpl aiqrfiiplp ihfripqpvp isvekvveks vpvpvpipve kviektvhhp
      781 rphpldmeki rpypletpkl vktapappli ysdgnfqqir qqnfqaplgp syeknhynst
      841 qfygsgylav nrpfvrqslv halpkkfgsy gtqyphslvy sslngnngnl vfygrsftek
      901 dkimdeyvgp vprkvqlslg iqsksiqyap pdvqstmrrt rqegntgsfr qskmeygfkp
      961 pmvpsiqyde qtatkve
//
LOCUS       XP_076545726             977 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606417 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545726
VERSION     XP_076545726.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689611.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..977
                     /product="uncharacterized protein LOC117606417 isoform X1"
                     /calculated_mol_wt=110449
     CDS             1..977
                     /gene="LOC117606417"
                     /coded_by="XM_076689611.1:1245..4178"
                     /db_xref="GeneID:117606417"
ORIGIN      
        1 mklnyivavl iwirtvtatl esdsrgnwsn ekelnyeqer ignqtkeeit gdvhspheri
       61 filssestes ikaerpeeld vkfanknedn hevnkqlakq lvqpymlplp letryfqqdt
      121 hnesrknvsr lartsqnspn etatveyips keilvqdstt tsnnypiqdk fndlaysnse
      181 dqadfeyvge fgqrvrssps frpksnenvg rdrfrpldtl slsedyrkhe rknsrpfvik
      241 klnasdsian lyskdrhpvv asgkasefqr gpkpkktgge eeeeysdtid ynkenvvsss
      301 skdttsppvq qvsfgkftgp ivvadlpqqk rysfatttgy tiindqpqsi pasleiiksk
      361 aaessylats savlnplqvg vammnvgqdi nsvndpvtft keylrnepdp sqlpvttdie
      421 siiqndaaid sgnsslqtdq lsqqeeiskv vasngptqsv eiqksvevfh tapvheihyp
      481 lefvpyvqqs snfkqrqhtd yrkpqggqek dqrpnvyknn eildetvssn npersryeyt
      541 vneddvvqvd qtvahstdvk pvfdareqle dvqdnqtagk yskiqripdt iategiakne
      601 hdpvkgvpll ntqpvgiidn lpalgnienp qasqqvpeil likptnepst evrflmavpq
      661 hypgekvhvh rtvnvdekkv pyhiekviek qitipqpfpv qvpvdrvvek qiripypvhv
      721 ekviekkvpl aiqrfiiplp ihfripqpvp isvekvveks vpvpvpipve kviektvhhp
      781 rphpldmeki rpypletpkl vktapappli ysdgnfqqir qqnfqaplgp syeknhynst
      841 qfygsgylav nrpfvrqslv halpkkfgsy gtqyphslvy sslngnngnl vfygrsftek
      901 dkimdeyvgp vprkvqlslg iqsksiqyap pdvqstmrrt rqegntgsfr qskmeygfkp
      961 pmvpsiqyde qtatkve
//
LOCUS       XP_034184700             977 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606417 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184700
VERSION     XP_034184700.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328809.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184700.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..977
                     /product="uncharacterized protein LOC117606417 isoform X1"
                     /calculated_mol_wt=110449
     CDS             1..977
                     /gene="LOC117606417"
                     /coded_by="XM_034328809.2:1310..4243"
                     /db_xref="GeneID:117606417"
ORIGIN      
        1 mklnyivavl iwirtvtatl esdsrgnwsn ekelnyeqer ignqtkeeit gdvhspheri
       61 filssestes ikaerpeeld vkfanknedn hevnkqlakq lvqpymlplp letryfqqdt
      121 hnesrknvsr lartsqnspn etatveyips keilvqdstt tsnnypiqdk fndlaysnse
      181 dqadfeyvge fgqrvrssps frpksnenvg rdrfrpldtl slsedyrkhe rknsrpfvik
      241 klnasdsian lyskdrhpvv asgkasefqr gpkpkktgge eeeeysdtid ynkenvvsss
      301 skdttsppvq qvsfgkftgp ivvadlpqqk rysfatttgy tiindqpqsi pasleiiksk
      361 aaessylats savlnplqvg vammnvgqdi nsvndpvtft keylrnepdp sqlpvttdie
      421 siiqndaaid sgnsslqtdq lsqqeeiskv vasngptqsv eiqksvevfh tapvheihyp
      481 lefvpyvqqs snfkqrqhtd yrkpqggqek dqrpnvyknn eildetvssn npersryeyt
      541 vneddvvqvd qtvahstdvk pvfdareqle dvqdnqtagk yskiqripdt iategiakne
      601 hdpvkgvpll ntqpvgiidn lpalgnienp qasqqvpeil likptnepst evrflmavpq
      661 hypgekvhvh rtvnvdekkv pyhiekviek qitipqpfpv qvpvdrvvek qiripypvhv
      721 ekviekkvpl aiqrfiiplp ihfripqpvp isvekvveks vpvpvpipve kviektvhhp
      781 rphpldmeki rpypletpkl vktapappli ysdgnfqqir qqnfqaplgp syeknhynst
      841 qfygsgylav nrpfvrqslv halpkkfgsy gtqyphslvy sslngnngnl vfygrsftek
      901 dkimdeyvgp vprkvqlslg iqsksiqyap pdvqstmrrt rqegntgsfr qskmeygfkp
      961 pmvpsiqyde qtatkve
//
LOCUS       XP_034184701             977 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606417 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184701
VERSION     XP_034184701.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328810.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184701.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..977
                     /product="uncharacterized protein LOC117606417 isoform X1"
                     /calculated_mol_wt=110449
     CDS             1..977
                     /gene="LOC117606417"
                     /coded_by="XM_034328810.2:410..3343"
                     /db_xref="GeneID:117606417"
ORIGIN      
        1 mklnyivavl iwirtvtatl esdsrgnwsn ekelnyeqer ignqtkeeit gdvhspheri
       61 filssestes ikaerpeeld vkfanknedn hevnkqlakq lvqpymlplp letryfqqdt
      121 hnesrknvsr lartsqnspn etatveyips keilvqdstt tsnnypiqdk fndlaysnse
      181 dqadfeyvge fgqrvrssps frpksnenvg rdrfrpldtl slsedyrkhe rknsrpfvik
      241 klnasdsian lyskdrhpvv asgkasefqr gpkpkktgge eeeeysdtid ynkenvvsss
      301 skdttsppvq qvsfgkftgp ivvadlpqqk rysfatttgy tiindqpqsi pasleiiksk
      361 aaessylats savlnplqvg vammnvgqdi nsvndpvtft keylrnepdp sqlpvttdie
      421 siiqndaaid sgnsslqtdq lsqqeeiskv vasngptqsv eiqksvevfh tapvheihyp
      481 lefvpyvqqs snfkqrqhtd yrkpqggqek dqrpnvyknn eildetvssn npersryeyt
      541 vneddvvqvd qtvahstdvk pvfdareqle dvqdnqtagk yskiqripdt iategiakne
      601 hdpvkgvpll ntqpvgiidn lpalgnienp qasqqvpeil likptnepst evrflmavpq
      661 hypgekvhvh rtvnvdekkv pyhiekviek qitipqpfpv qvpvdrvvek qiripypvhv
      721 ekviekkvpl aiqrfiiplp ihfripqpvp isvekvveks vpvpvpipve kviektvhhp
      781 rphpldmeki rpypletpkl vktapappli ysdgnfqqir qqnfqaplgp syeknhynst
      841 qfygsgylav nrpfvrqslv halpkkfgsy gtqyphslvy sslngnngnl vfygrsftek
      901 dkimdeyvgp vprkvqlslg iqsksiqyap pdvqstmrrt rqegntgsfr qskmeygfkp
      961 pmvpsiqyde qtatkve
//
LOCUS       XP_034184702             977 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606417 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184702
VERSION     XP_034184702.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328811.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184702.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..977
                     /product="uncharacterized protein LOC117606417 isoform X1"
                     /calculated_mol_wt=110449
     CDS             1..977
                     /gene="LOC117606417"
                     /coded_by="XM_034328811.2:1192..4125"
                     /db_xref="GeneID:117606417"
ORIGIN      
        1 mklnyivavl iwirtvtatl esdsrgnwsn ekelnyeqer ignqtkeeit gdvhspheri
       61 filssestes ikaerpeeld vkfanknedn hevnkqlakq lvqpymlplp letryfqqdt
      121 hnesrknvsr lartsqnspn etatveyips keilvqdstt tsnnypiqdk fndlaysnse
      181 dqadfeyvge fgqrvrssps frpksnenvg rdrfrpldtl slsedyrkhe rknsrpfvik
      241 klnasdsian lyskdrhpvv asgkasefqr gpkpkktgge eeeeysdtid ynkenvvsss
      301 skdttsppvq qvsfgkftgp ivvadlpqqk rysfatttgy tiindqpqsi pasleiiksk
      361 aaessylats savlnplqvg vammnvgqdi nsvndpvtft keylrnepdp sqlpvttdie
      421 siiqndaaid sgnsslqtdq lsqqeeiskv vasngptqsv eiqksvevfh tapvheihyp
      481 lefvpyvqqs snfkqrqhtd yrkpqggqek dqrpnvyknn eildetvssn npersryeyt
      541 vneddvvqvd qtvahstdvk pvfdareqle dvqdnqtagk yskiqripdt iategiakne
      601 hdpvkgvpll ntqpvgiidn lpalgnienp qasqqvpeil likptnepst evrflmavpq
      661 hypgekvhvh rtvnvdekkv pyhiekviek qitipqpfpv qvpvdrvvek qiripypvhv
      721 ekviekkvpl aiqrfiiplp ihfripqpvp isvekvveks vpvpvpipve kviektvhhp
      781 rphpldmeki rpypletpkl vktapappli ysdgnfqqir qqnfqaplgp syeknhynst
      841 qfygsgylav nrpfvrqslv halpkkfgsy gtqyphslvy sslngnngnl vfygrsftek
      901 dkimdeyvgp vprkvqlslg iqsksiqyap pdvqstmrrt rqegntgsfr qskmeygfkp
      961 pmvpsiqyde qtatkve
//
LOCUS       XP_034184703             953 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606417 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184703
VERSION     XP_034184703.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328812.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184703.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..953
                     /product="uncharacterized protein LOC117606417 isoform X2"
                     /calculated_mol_wt=107670
     CDS             1..953
                     /gene="LOC117606417"
                     /coded_by="XM_034328812.2:1333..4194"
                     /db_xref="GeneID:117606417"
ORIGIN      
        1 mklnyivavl iwirtvtatl esdsrgnwsn ekelnyeqer ignqtkeeit gdvhspheri
       61 filssestes ikaerpeeld vkfanknedn hevnkqlakq lvqpymlplp letryfqqdt
      121 hnesrknvsr lartsqnspn etatveyips keilvqdstt tsnnypiqdk fndlaysnse
      181 dqadfeyvge fgqrvrssps frpksnenvg rdrfrpldtl slsedyrkhe rknsrpfvik
      241 klnasdsian lyskdrhpvv asgkasefqr gpkpkktgge eeeeysdtid ynkenvvsss
      301 skdttsppvq qvsfgkftgp ivvadlpqqk rysfatttgy tiindqpqsi pasleiiksk
      361 aaessylats savlnplqvg vammnvgqdi nsvndpvtft keylrnepdp sqlpvttdie
      421 siiqndaaid sgnsslqtdq lsqqeeiskv vasngptqsv eiqksvevfh tapvheihyp
      481 lefvpyvqqs snfkqrqhtd yrkpqggqek dqrpnvyknn eildetvssn npersryeyt
      541 vneddvvqvd qtvahstdvk pvfdareqle dvqdnqtagk yskiqripdt iategiakne
      601 hdpvkgvpll ntqpvgiidn lpalgnienp qasqqvpeil likptnepst evrflmavpq
      661 hypgekvhvh rtvnvdekkv pyhiekviek qitipqpfpv qvpvdrvvek qiripypvhv
      721 ekviekkvpl aiqrfiiplp ihfripqpvp isvekvveks vpvpvpipve kviektvhhp
      781 rphpldmeki rpypletpkl vktapappli ysdgnfqqir qqnfqaplgp syeknhynst
      841 qfygsgylav nrpfvrqslv halpkkfgsy gtqyphslvy sslngpvprk vqlslgiqsk
      901 siqyappdvq stmrrtrqeg ntgsfrqskm eygfkppmvp siqydeqtat kve
//
LOCUS       XP_034184704             872 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606417 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184704
VERSION     XP_034184704.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328813.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184704.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..872
                     /product="uncharacterized protein LOC117606417 isoform X3"
                     /calculated_mol_wt=98254
     CDS             1..872
                     /gene="LOC117606417"
                     /coded_by="XM_034328813.2:783..3401"
                     /db_xref="GeneID:117606417"
ORIGIN      
        1 mlplpletry fqqdthnesr knvsrlarts qnspnetatv eyipskeilv qdstttsnny
       61 piqdkfndla ysnsedqadf eyvgefgqrv rsspsfrpks nenvgrdrfr pldtlslsed
      121 yrkherknsr pfvikklnas dsianlyskd rhpvvasgka sefqrgpkpk ktggeeeeey
      181 sdtidynken vvsssskdtt sppvqqvsfg kftgpivvad lpqqkrysfa tttgytiind
      241 qpqsipasle iikskaaess ylatssavln plqvgvammn vgqdinsvnd pvtftkeylr
      301 nepdpsqlpv ttdiesiiqn daaidsgnss lqtdqlsqqe eiskvvasng ptqsveiqks
      361 vevfhtapvh eihyplefvp yvqqssnfkq rqhtdyrkpq ggqekdqrpn vyknneilde
      421 tvssnnpers ryeytvnedd vvqvdqtvah stdvkpvfda reqledvqdn qtagkyskiq
      481 ripdtiateg iaknehdpvk gvpllntqpv giidnlpalg nienpqasqq vpeillikpt
      541 nepstevrfl mavpqhypge kvhvhrtvnv dekkvpyhie kviekqitip qpfpvqvpvd
      601 rvvekqirip ypvhvekvie kkvplaiqrf iiplpihfri pqpvpisvek vveksvpvpv
      661 pipvekviek tvhhprphpl dmekirpypl etpklvktap appliysdgn fqqirqqnfq
      721 aplgpsyekn hynstqfygs gylavnrpfv rqslvhalpk kfgsygtqyp hslvysslng
      781 nngnlvfygr sftekdkimd eyvgpvprkv qlslgiqsks iqyappdvqs tmrrtrqegn
      841 tgsfrqskme ygfkppmvps iqydeqtatk ve
//
LOCUS       XP_034184705             823 aa            linear   INV 03-SEP-2025
DEFINITION  aftiphilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184705
VERSION     XP_034184705.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328814.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184705.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="aftiphilin isoform X1"
                     /calculated_mol_wt=91286
     Region          195..>307
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          498..570
                     /region_name="Clathrin_bdg"
                     /note="Clathrin-binding box of Aftiphilin, vesicle
                     trafficking; pfam15045"
                     /db_xref="CDD:464467"
     CDS             1..823
                     /gene="Afti"
                     /coded_by="XM_034328814.2:241..2712"
                     /db_xref="GeneID:117606419"
ORIGIN      
        1 mafpplvsst pppldnfgds eedefgdftt ggidglsvss dspqkpitpi qtpltsqnps
       61 pkvngvlend nnqssihvkt tvpeeflile kidetisnik lkteveniye shdrkknven
      121 anisstsssn stssssedat ekgvnltsgs ssfggseqet spnhlediep lsldlgdaaa
      181 aaaaaaaadt vqsidddfyd yeqfedsqnw vpnneestgv sntdyfkfqe penitnnlna
      241 lnkdinvsdk kdvnlkfeqs veeskldhcv enkdvptedk nvfefkvndp kfsstenpvf
      301 hdssnetfne tvnenenete vaedlhefse nenntvennf lssaplanvs ncsseefddf
      361 kydfvfgtst ktsqpefsnc stvetnvkrk etmgedddfg dftnfanqtq fdenvdetnv
      421 pdkdddddfg dfndfesave pavepavveq pqfslkesic rienknaank iediittmfl
      481 mdvnqleiea qplisetdkv wksiknveet naltyqwans ssnkmllnsl gidsrnilfg
      541 prwnpnvprf aanlgftple pikattdiqs attsninktq tstnseevpa aqfdwnssgl
      601 vnpleasggl sallpldflc pfdplltshn snnsesyrrp ssarnpanhh itemnrercn
      661 svskvetidr legdglksqk rsqtskiiep lpaprttewk rkgdfesgqk vrsstggqrs
      721 vqtekqclpm dkqfltnerq yvsaeksstl gdsyrgksmn krstsehvvm drfgravpiq
      781 aetarvlnrl pdlsflsart lmldreqkql acemgimsrk mpg
//
LOCUS       XP_034184706             823 aa            linear   INV 03-SEP-2025
DEFINITION  aftiphilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184706
VERSION     XP_034184706.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328815.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184706.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="aftiphilin isoform X1"
                     /calculated_mol_wt=91286
     Region          195..>307
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          498..570
                     /region_name="Clathrin_bdg"
                     /note="Clathrin-binding box of Aftiphilin, vesicle
                     trafficking; pfam15045"
                     /db_xref="CDD:464467"
     CDS             1..823
                     /gene="Afti"
                     /coded_by="XM_034328815.2:171..2642"
                     /db_xref="GeneID:117606419"
ORIGIN      
        1 mafpplvsst pppldnfgds eedefgdftt ggidglsvss dspqkpitpi qtpltsqnps
       61 pkvngvlend nnqssihvkt tvpeeflile kidetisnik lkteveniye shdrkknven
      121 anisstsssn stssssedat ekgvnltsgs ssfggseqet spnhlediep lsldlgdaaa
      181 aaaaaaaadt vqsidddfyd yeqfedsqnw vpnneestgv sntdyfkfqe penitnnlna
      241 lnkdinvsdk kdvnlkfeqs veeskldhcv enkdvptedk nvfefkvndp kfsstenpvf
      301 hdssnetfne tvnenenete vaedlhefse nenntvennf lssaplanvs ncsseefddf
      361 kydfvfgtst ktsqpefsnc stvetnvkrk etmgedddfg dftnfanqtq fdenvdetnv
      421 pdkdddddfg dfndfesave pavepavveq pqfslkesic rienknaank iediittmfl
      481 mdvnqleiea qplisetdkv wksiknveet naltyqwans ssnkmllnsl gidsrnilfg
      541 prwnpnvprf aanlgftple pikattdiqs attsninktq tstnseevpa aqfdwnssgl
      601 vnpleasggl sallpldflc pfdplltshn snnsesyrrp ssarnpanhh itemnrercn
      661 svskvetidr legdglksqk rsqtskiiep lpaprttewk rkgdfesgqk vrsstggqrs
      721 vqtekqclpm dkqfltnerq yvsaeksstl gdsyrgksmn krstsehvvm drfgravpiq
      781 aetarvlnrl pdlsflsart lmldreqkql acemgimsrk mpg
//
LOCUS       XP_034184707             823 aa            linear   INV 03-SEP-2025
DEFINITION  aftiphilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184707
VERSION     XP_034184707.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328816.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184707.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="aftiphilin isoform X1"
                     /calculated_mol_wt=91286
     Region          195..>307
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          498..570
                     /region_name="Clathrin_bdg"
                     /note="Clathrin-binding box of Aftiphilin, vesicle
                     trafficking; pfam15045"
                     /db_xref="CDD:464467"
     CDS             1..823
                     /gene="Afti"
                     /coded_by="XM_034328816.2:157..2628"
                     /db_xref="GeneID:117606419"
ORIGIN      
        1 mafpplvsst pppldnfgds eedefgdftt ggidglsvss dspqkpitpi qtpltsqnps
       61 pkvngvlend nnqssihvkt tvpeeflile kidetisnik lkteveniye shdrkknven
      121 anisstsssn stssssedat ekgvnltsgs ssfggseqet spnhlediep lsldlgdaaa
      181 aaaaaaaadt vqsidddfyd yeqfedsqnw vpnneestgv sntdyfkfqe penitnnlna
      241 lnkdinvsdk kdvnlkfeqs veeskldhcv enkdvptedk nvfefkvndp kfsstenpvf
      301 hdssnetfne tvnenenete vaedlhefse nenntvennf lssaplanvs ncsseefddf
      361 kydfvfgtst ktsqpefsnc stvetnvkrk etmgedddfg dftnfanqtq fdenvdetnv
      421 pdkdddddfg dfndfesave pavepavveq pqfslkesic rienknaank iediittmfl
      481 mdvnqleiea qplisetdkv wksiknveet naltyqwans ssnkmllnsl gidsrnilfg
      541 prwnpnvprf aanlgftple pikattdiqs attsninktq tstnseevpa aqfdwnssgl
      601 vnpleasggl sallpldflc pfdplltshn snnsesyrrp ssarnpanhh itemnrercn
      661 svskvetidr legdglksqk rsqtskiiep lpaprttewk rkgdfesgqk vrsstggqrs
      721 vqtekqclpm dkqfltnerq yvsaeksstl gdsyrgksmn krstsehvvm drfgravpiq
      781 aetarvlnrl pdlsflsart lmldreqkql acemgimsrk mpg
//
LOCUS       XP_034184708             823 aa            linear   INV 03-SEP-2025
DEFINITION  aftiphilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184708
VERSION     XP_034184708.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328817.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184708.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="aftiphilin isoform X1"
                     /calculated_mol_wt=91286
     Region          195..>307
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          498..570
                     /region_name="Clathrin_bdg"
                     /note="Clathrin-binding box of Aftiphilin, vesicle
                     trafficking; pfam15045"
                     /db_xref="CDD:464467"
     CDS             1..823
                     /gene="Afti"
                     /coded_by="XM_034328817.2:354..2825"
                     /db_xref="GeneID:117606419"
ORIGIN      
        1 mafpplvsst pppldnfgds eedefgdftt ggidglsvss dspqkpitpi qtpltsqnps
       61 pkvngvlend nnqssihvkt tvpeeflile kidetisnik lkteveniye shdrkknven
      121 anisstsssn stssssedat ekgvnltsgs ssfggseqet spnhlediep lsldlgdaaa
      181 aaaaaaaadt vqsidddfyd yeqfedsqnw vpnneestgv sntdyfkfqe penitnnlna
      241 lnkdinvsdk kdvnlkfeqs veeskldhcv enkdvptedk nvfefkvndp kfsstenpvf
      301 hdssnetfne tvnenenete vaedlhefse nenntvennf lssaplanvs ncsseefddf
      361 kydfvfgtst ktsqpefsnc stvetnvkrk etmgedddfg dftnfanqtq fdenvdetnv
      421 pdkdddddfg dfndfesave pavepavveq pqfslkesic rienknaank iediittmfl
      481 mdvnqleiea qplisetdkv wksiknveet naltyqwans ssnkmllnsl gidsrnilfg
      541 prwnpnvprf aanlgftple pikattdiqs attsninktq tstnseevpa aqfdwnssgl
      601 vnpleasggl sallpldflc pfdplltshn snnsesyrrp ssarnpanhh itemnrercn
      661 svskvetidr legdglksqk rsqtskiiep lpaprttewk rkgdfesgqk vrsstggqrs
      721 vqtekqclpm dkqfltnerq yvsaeksstl gdsyrgksmn krstsehvvm drfgravpiq
      781 aetarvlnrl pdlsflsart lmldreqkql acemgimsrk mpg
//
LOCUS       XP_034184710             823 aa            linear   INV 03-SEP-2025
DEFINITION  aftiphilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184710
VERSION     XP_034184710.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328819.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184710.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="aftiphilin isoform X1"
                     /calculated_mol_wt=91286
     Region          195..>307
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          498..570
                     /region_name="Clathrin_bdg"
                     /note="Clathrin-binding box of Aftiphilin, vesicle
                     trafficking; pfam15045"
                     /db_xref="CDD:464467"
     CDS             1..823
                     /gene="Afti"
                     /coded_by="XM_034328819.2:350..2821"
                     /db_xref="GeneID:117606419"
ORIGIN      
        1 mafpplvsst pppldnfgds eedefgdftt ggidglsvss dspqkpitpi qtpltsqnps
       61 pkvngvlend nnqssihvkt tvpeeflile kidetisnik lkteveniye shdrkknven
      121 anisstsssn stssssedat ekgvnltsgs ssfggseqet spnhlediep lsldlgdaaa
      181 aaaaaaaadt vqsidddfyd yeqfedsqnw vpnneestgv sntdyfkfqe penitnnlna
      241 lnkdinvsdk kdvnlkfeqs veeskldhcv enkdvptedk nvfefkvndp kfsstenpvf
      301 hdssnetfne tvnenenete vaedlhefse nenntvennf lssaplanvs ncsseefddf
      361 kydfvfgtst ktsqpefsnc stvetnvkrk etmgedddfg dftnfanqtq fdenvdetnv
      421 pdkdddddfg dfndfesave pavepavveq pqfslkesic rienknaank iediittmfl
      481 mdvnqleiea qplisetdkv wksiknveet naltyqwans ssnkmllnsl gidsrnilfg
      541 prwnpnvprf aanlgftple pikattdiqs attsninktq tstnseevpa aqfdwnssgl
      601 vnpleasggl sallpldflc pfdplltshn snnsesyrrp ssarnpanhh itemnrercn
      661 svskvetidr legdglksqk rsqtskiiep lpaprttewk rkgdfesgqk vrsstggqrs
      721 vqtekqclpm dkqfltnerq yvsaeksstl gdsyrgksmn krstsehvvm drfgravpiq
      781 aetarvlnrl pdlsflsart lmldreqkql acemgimsrk mpg
//
LOCUS       XP_034184711             780 aa            linear   INV 03-SEP-2025
DEFINITION  aftiphilin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184711
VERSION     XP_034184711.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328820.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184711.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..780
                     /product="aftiphilin isoform X2"
                     /calculated_mol_wt=86627
     Region          195..>307
                     /region_name="PTZ00482"
                     /note="membrane-attack complex/perforin (MACPF)
                     Superfamily; Provisional"
                     /db_xref="CDD:240433"
     Region          498..570
                     /region_name="Clathrin_bdg"
                     /note="Clathrin-binding box of Aftiphilin, vesicle
                     trafficking; pfam15045"
                     /db_xref="CDD:464467"
     CDS             1..780
                     /gene="Afti"
                     /coded_by="XM_034328820.2:240..2582"
                     /db_xref="GeneID:117606419"
ORIGIN      
        1 mafpplvsst pppldnfgds eedefgdftt ggidglsvss dspqkpitpi qtpltsqnps
       61 pkvngvlend nnqssihvkt tvpeeflile kidetisnik lkteveniye shdrkknven
      121 anisstsssn stssssedat ekgvnltsgs ssfggseqet spnhlediep lsldlgdaaa
      181 aaaaaaaadt vqsidddfyd yeqfedsqnw vpnneestgv sntdyfkfqe penitnnlna
      241 lnkdinvsdk kdvnlkfeqs veeskldhcv enkdvptedk nvfefkvndp kfsstenpvf
      301 hdssnetfne tvnenenete vaedlhefse nenntvennf lssaplanvs ncsseefddf
      361 kydfvfgtst ktsqpefsnc stvetnvkrk etmgedddfg dftnfanqtq fdenvdetnv
      421 pdkdddddfg dfndfesave pavepavveq pqfslkesic rienknaank iediittmfl
      481 mdvnqleiea qplisetdkv wksiknveet naltyqwans ssnkmllnsl gidsrnilfg
      541 prwnpnvprf aanlgftple pikattdiqs attsninktq tstnseevpa aqfdwnssgl
      601 vnpleanite mnrercnsvs kvetidrleg dglksqkrsq tskiieplpa prttewkrkg
      661 dfesgqkvrs stggqrsvqt ekqclpmdkq fltnerqyvs aeksstlgds yrgksmnkrs
      721 tsehvvmdrf gravpiqaet arvlnrlpdl sflsartlml dreqkqlace mgimsrkmpg
//
LOCUS       XP_034184714             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606421 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184714
VERSION     XP_034184714.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328823.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184714.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117606421 isoform X1"
                     /calculated_mol_wt=85886
     Region          64..137
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); pfam07776"
                     /db_xref="CDD:462262"
     Region          287..308
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          316..336
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(321,323,325,327..328,331..332,335,349,351,355..356,
                     359..360,363,377,379,381,383..384,387..388)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          336..725
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          344..364
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          372..389
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          444..464
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          472..492
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          541..562
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          567..587
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          595..615
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          623..644
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          652..672
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(657,659,661,663..664,667..668,671,685,687,691..692,
                     695..696,699,713,715,717,719..720,723..724,727)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          680..700
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          708..727
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..736
                     /gene="LOC117606421"
                     /coded_by="XM_034328823.2:316..2526"
                     /db_xref="GeneID:117606421"
ORIGIN      
        1 mkkffpyhfq gtrkaesist ltehedeldd fegsivwsqe eplssneile ltsdtekqnr
       61 lkslcrlcag etpnpiyiys efgeslrlld kintclsikv kktdplpkqi cpmcvekitw
      121 cnefdaqcir aeetlfeilk dkhsftntqn senaqgntds cplciegrmr vyeedkenrt
      181 dielydsdiv lessdeeqip neikadedla nesitlfclg tkqkylkcga cmnlyhtket
      241 ldehkckpnl qctskpykcd vcfatftfee rlqfhqhfhr nakalyceic kitfgkelkl
      301 fyhykryhck dgrvsclqcg klfenqeglk khvcvdgktr phvcevcskg fcdgytlkrh
      361 vvthlpekpy kcpecsksft qksrlnkhva ghsivfensq tiwrcihcge vfgscecade
      421 hcknheekvv ncieelqvlk lyhcefcnsh fadmdhlknh reshtiekpy scnhctssfk
      481 sfaeavlhwk ehprifkvlv vfvceicdrd vkelsllykh kqkrhqpipr ksekgekkfi
      541 celcgnifai ieefqehgky rcskfpcdic gsllptansl nahkrrhsgl rpyvcnicgk
      601 sytqsshmwt hkrfhmgvkp yaceycdqrf tikpdladht rkkhtrerpf kcdvcnkafl
      661 tgsvfyqhrl ihrgdrrykc hhcekaftrt ealnnhikih tgekphacdv cgrcfrqkgd
      721 mrkhrrtqht akqdtk
//
LOCUS       XP_034184715             727 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606421 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184715
VERSION     XP_034184715.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328824.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184715.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..727
                     /product="uncharacterized protein LOC117606421 isoform X2"
                     /calculated_mol_wt=84731
     Region          55..128
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); pfam07776"
                     /db_xref="CDD:462262"
     Region          278..299
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          307..327
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(312,314,316,318..319,322..323,326,340,342,346..347,
                     350..351,354,368,370,372,374..375,378..379)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          327..716
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          335..355
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          363..380
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          435..455
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          463..483
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          532..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          558..578
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          586..606
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          614..635
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          643..663
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(648,650,652,654..655,658..659,662,676,678,682..683,
                     686..687,690,704,706,708,710..711,714..715,718)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          671..691
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          699..718
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..727
                     /gene="LOC117606421"
                     /coded_by="XM_034328824.2:423..2606"
                     /db_xref="GeneID:117606421"
ORIGIN      
        1 mnirkaesis tltehedeld dfegsivwsq eeplssneil eltsdtekqn rlkslcrlca
       61 getpnpiyiy sefgeslrll dkintclsik vkktdplpkq icpmcvekit wcnefdaqci
      121 raeetlfeil kdkhsftntq nsenaqgntd scplciegrm rvyeedkenr tdielydsdi
      181 vlessdeeqi pneikadedl anesitlfcl gtkqkylkcg acmnlyhtke tldehkckpn
      241 lqctskpykc dvcfatftfe erlqfhqhfh rnakalycei ckitfgkelk lfyhykryhc
      301 kdgrvsclqc gklfenqegl kkhvcvdgkt rphvcevcsk gfcdgytlkr hvvthlpekp
      361 ykcpecsksf tqksrlnkhv aghsivfens qtiwrcihcg evfgscecad ehcknheekv
      421 vncieelqvl klyhcefcns hfadmdhlkn hreshtiekp yscnhctssf ksfaeavlhw
      481 kehprifkvl vvfvceicdr dvkelsllyk hkqkrhqpip rksekgekkf icelcgnifa
      541 iieefqehgk yrcskfpcdi cgsllptans lnahkrrhsg lrpyvcnicg ksytqsshmw
      601 thkrfhmgvk pyaceycdqr ftikpdladh trkkhtrerp fkcdvcnkaf ltgsvfyqhr
      661 lihrgdrryk chhcekaftr tealnnhiki htgekphacd vcgrcfrqkg dmrkhrrtqh
      721 takqdtk
//
LOCUS       XP_034184716             670 aa            linear   INV 03-SEP-2025
DEFINITION  myotubularin related protein 6 [Osmia lignaria lignaria].
ACCESSION   XP_034184716
VERSION     XP_034184716.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328825.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..670
                     /product="myotubularin related protein 6"
                     /calculated_mol_wt=77191
     Region          4..100
                     /region_name="PH-GRAM_MTMR6-like"
                     /note="Myotubularian (MTM) related (MTMR) 7 and 8 proteins
                     Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase
                     activators and Myotubularins (PH-GRAM) domain; cd13210"
                     /db_xref="CDD:270030"
     Region          130..426
                     /region_name="PTP-MTMR6-like"
                     /note="protein tyrosine phosphatase-like domain of
                     myotubularin related phosphoinositide phosphatases 6, 7,
                     and 8; cd14532"
                     /db_xref="CDD:350380"
     Site            order(289..292,294..295,299,304)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:350380"
     Region          600..659
                     /region_name="FYVE_MTMR_unchar"
                     /note="FYVE-related domain found in uncharacterized
                     myotubularin-related proteins mainly from eumetazoa;
                     cd15738"
                     /db_xref="CDD:277277"
     CDS             1..670
                     /gene="Mtmr6"
                     /coded_by="XM_034328825.2:117..2129"
                     /db_xref="GeneID:117606423"
ORIGIN      
        1 mdqikipkve nvrildkysn nhsigtlylt vthlifteqs gkkkiwvlyt hisniekqpl
       61 tttgsplcik ckhffivtfv ipkerdchdi yltlsklscp aniedlycfn yqaskdtlpq
      121 hagwnffnvq sefqrqgvpn dewsltylnt nyelcdtypk ylyvpstcan stligsakfr
      181 srgrlpvlty lhsnkaavcr csqplsgfsa rcpedeqmmy nilrtnfnsk ymyvvdtrpr
      241 inafanraag kgyenenfyd nikfhffgie nihvmrtsln klielqrttt msaflsgles
      301 sgwlkhirsi letawfiara vsngvsvvvh csdgwdrtaq vcslsallld pfyrtiqgfq
      361 aliekdwlsf ghkfsdrcgh incdskelap iftqfidaty qllqqyphkf qfnefflltl
      421 hdhvhscqhg tfignseker hllrvserty slwgylannm heyinplykc nrfnkesnfv
      481 lqpklaphsi alwrgmyfrf engihprety edfllvmhdh tscledhvkl llkrvgslns
      541 iinlnnvqkk giqgkmkfdn kftkdplsdt iientanhee ktksklkiaq lenelktvsl
      601 dwkllrniee ctcsttfdaf nrkhhcwscg evlctrcmga htvlaghfsq ravptcksci
      661 qnssipsasp
//
LOCUS       XP_034184719             629 aa            linear   INV 03-SEP-2025
DEFINITION  bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184719
VERSION     XP_034184719.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328828.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184719.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="bifunctional 3'-phosphoadenosine
                     5'-phosphosulfate synthase isoform X1"
                     /calculated_mol_wt=71724
     Region          31..225
                     /region_name="CysC"
                     /note="Adenylylsulfate kinase or related kinase [Inorganic
                     ion transport and metabolism]; COG0529"
                     /db_xref="CDD:440295"
     Region          253..620
                     /region_name="ATPS"
                     /note="ATP-sulfurylase; cd00517"
                     /db_xref="CDD:173895"
     Site            order(417..421,427,430,494,519..521,523..524,564..566)
                     /site_type="active"
                     /db_xref="CDD:173895"
     Site            424..427
                     /site_type="other"
                     /note="HXXH motif"
                     /db_xref="CDD:173895"
     Site            456..466
                     /site_type="other"
                     /note="flexible loop"
                     /db_xref="CDD:173895"
     CDS             1..629
                     /gene="LOC117606424"
                     /coded_by="XM_034328828.2:542..2431"
                     /db_xref="GeneID:117606424"
ORIGIN      
        1 mgmyiknlme drnserknnq irstnvteqi hqvsrekrge hygkfkrfrg ctiwltglsg
       61 agktsisfql enhlvsqgip aysldgdnir gglnrnlgfs kedreenvrr aaevaklfad
      121 cgiiticsfv spfevdrrla rsiheefnlr ffeifvqasv etceardvkg lyekarkgai
      181 ksftgigqdy eapkhadfvv ntedhsldts kkmvidflik egiipmipep veelfipeek
      241 lkdakreadk lfsieindid lqwiqvlaeg wasplrgfmr edqylkcqhf kiieegqssv
      301 inqsipivlp vttaqkekct nstaltlkyq driiailrkp effahrkeer ccrefgtndp
      361 ghpyvrmihn sgdwliggel evfqrirwnd gldkyrltpn eirkmckkmg adavfafqlr
      421 npihnghall mqdtrrylve erafkkpvll lhplggwtke ddvplsvrin qhqsvleegv
      481 lhndtilaif pspmlyagpt evqwhakarm magatfyivg rdpaglphpd ksktpdgnly
      541 dgthgskvls mapgleglei ipfrvaaydn rarrmaffep ersqdfsfis gtkmralaks
      601 gekppegfma pkawrivady yqeklrnnn
//
LOCUS       XP_034184720             625 aa            linear   INV 03-SEP-2025
DEFINITION  bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184720
VERSION     XP_034184720.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328829.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184720.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..625
                     /product="bifunctional 3'-phosphoadenosine
                     5'-phosphosulfate synthase isoform X2"
                     /calculated_mol_wt=71055
     Region          27..221
                     /region_name="CysC"
                     /note="Adenylylsulfate kinase or related kinase [Inorganic
                     ion transport and metabolism]; COG0529"
                     /db_xref="CDD:440295"
     Region          249..616
                     /region_name="ATPS"
                     /note="ATP-sulfurylase; cd00517"
                     /db_xref="CDD:173895"
     Site            order(413..417,423,426,490,515..517,519..520,560..562)
                     /site_type="active"
                     /db_xref="CDD:173895"
     Site            420..423
                     /site_type="other"
                     /note="HXXH motif"
                     /db_xref="CDD:173895"
     Site            452..462
                     /site_type="other"
                     /note="flexible loop"
                     /db_xref="CDD:173895"
     CDS             1..625
                     /gene="LOC117606424"
                     /coded_by="XM_034328829.2:445..2322"
                     /db_xref="GeneID:117606424"
ORIGIN      
        1 mngksemkss sepinyirst nvteqihqvs rekrgehygk fkrfrgctiw ltglsgagkt
       61 sisfqlenhl vsqgipaysl dgdnirggln rnlgfskedr eenvrraaev aklfadcgii
      121 ticsfvspfe vdrrlarsih eefnlrffei fvqasvetce ardvkglyek arkgaiksft
      181 gigqdyeapk hadfvvnted hsldtskkmv idflikegii pmipepveel fipeeklkda
      241 kreadklfsi eindidlqwi qvlaegwasp lrgfmredqy lkcqhfkiie egqssvinqs
      301 ipivlpvtta qkekctnsta ltlkyqdrii ailrkpeffa hrkeerccre fgtndpghpy
      361 vrmihnsgdw liggelevfq rirwndgldk yrltpneirk mckkmgadav fafqlrnpih
      421 nghallmqdt rrylveeraf kkpvlllhpl ggwtkeddvp lsvrinqhqs vleegvlhnd
      481 tilaifpspm lyagptevqw hakarmmaga tfyivgrdpa glphpdkskt pdgnlydgth
      541 gskvlsmapg legleiipfr vaaydnrarr maffepersq dfsfisgtkm ralaksgekp
      601 pegfmapkaw rivadyyqek lrnnn
//
LOCUS       XP_034184721             610 aa            linear   INV 03-SEP-2025
DEFINITION  visceral mesodermal armadillo-repeats isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184721
VERSION     XP_034184721.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328830.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184721.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="visceral mesodermal armadillo-repeats isoform
                     X1"
                     /calculated_mol_wt=67742
     Region          <57..>209
                     /region_name="PLN03200"
                     /note="cellulose synthase-interactive protein;
                     Provisional"
                     /db_xref="CDD:215629"
     Site            order(69,73,77,110,114,118,150,154,160)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          85..121
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          127..161
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <324..>538
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     CDS             1..610
                     /gene="vimar"
                     /coded_by="XM_034328830.2:305..2137"
                     /db_xref="GeneID:117606425"
ORIGIN      
        1 megelkhnid klveelrfvv ksekeikdii qvldsivnvs ktsigeatef tiddvfltll
       61 rheskeiiak takaiaeiak sergrekcts tdlvnalidl lkdervdvlt qtsralgnic
      121 yenengkkfv edknglmsil avleksvtlk nvegapflrn vavglllnfl idrpslhkea
      181 lqqdivpiic sileidgatg geaamhalli lgisndaniv flderltril vdilgtdass
      241 elsemclelf hgqaedekak lllanagvce lllkllekhs pyctdeetrc vlkiacnliv
      301 lvltgddsmn slydnsngiv ykklvewles edrdlqitav lamgnfartd ahcklmvtqn
      361 vhctllkllq dnntdtadir fqhallsalr nlvipvdnka vilkdgllni lypminissy
      421 pvvfkllgtl rividgqsea aielgkkdgl vekvvewcev eehpgvqgea nrliawlinn
      481 srdkgitvsv ieygaikhlv kmltaqhvlm qnealislti tttisleece kslleadvge
      541 klckffnpqh nleipilhnv lsllvnivns dqlrkhlgkt nlitlcreti rvkedsslqe
      601 klekicdkii
//
LOCUS       XP_076545727             610 aa            linear   INV 03-SEP-2025
DEFINITION  visceral mesodermal armadillo-repeats isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545727
VERSION     XP_076545727.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689612.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="visceral mesodermal armadillo-repeats isoform
                     X1"
                     /calculated_mol_wt=67742
     Region          <57..>209
                     /region_name="PLN03200"
                     /note="cellulose synthase-interactive protein;
                     Provisional"
                     /db_xref="CDD:215629"
     Site            order(69,73,77,110,114,118,150,154,160)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          85..121
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          127..161
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <324..>538
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     CDS             1..610
                     /gene="vimar"
                     /coded_by="XM_076689612.1:229..2061"
                     /db_xref="GeneID:117606425"
ORIGIN      
        1 megelkhnid klveelrfvv ksekeikdii qvldsivnvs ktsigeatef tiddvfltll
       61 rheskeiiak takaiaeiak sergrekcts tdlvnalidl lkdervdvlt qtsralgnic
      121 yenengkkfv edknglmsil avleksvtlk nvegapflrn vavglllnfl idrpslhkea
      181 lqqdivpiic sileidgatg geaamhalli lgisndaniv flderltril vdilgtdass
      241 elsemclelf hgqaedekak lllanagvce lllkllekhs pyctdeetrc vlkiacnliv
      301 lvltgddsmn slydnsngiv ykklvewles edrdlqitav lamgnfartd ahcklmvtqn
      361 vhctllkllq dnntdtadir fqhallsalr nlvipvdnka vilkdgllni lypminissy
      421 pvvfkllgtl rividgqsea aielgkkdgl vekvvewcev eehpgvqgea nrliawlinn
      481 srdkgitvsv ieygaikhlv kmltaqhvlm qnealislti tttisleece kslleadvge
      541 klckffnpqh nleipilhnv lsllvnivns dqlrkhlgkt nlitlcreti rvkedsslqe
      601 klekicdkii
//
LOCUS       XP_034184722             567 aa            linear   INV 03-SEP-2025
DEFINITION  visceral mesodermal armadillo-repeats isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184722
VERSION     XP_034184722.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184722.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..567
                     /product="visceral mesodermal armadillo-repeats isoform
                     X2"
                     /calculated_mol_wt=62895
     Region          <14..>166
                     /region_name="PLN03200"
                     /note="cellulose synthase-interactive protein;
                     Provisional"
                     /db_xref="CDD:215629"
     Site            order(26,30,34,67,71,75,107,111,117)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          42..78
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          84..118
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <281..>495
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     CDS             1..567
                     /gene="vimar"
                     /coded_by="XM_034328831.2:305..2008"
                     /db_xref="GeneID:117606425"
ORIGIN      
        1 meeateftid dvfltllrhe skeiiaktak aiaeiakser grekctstdl vnalidllkd
       61 ervdvltqts ralgnicyen engkkfvedk nglmsilavl eksvtlknve gapflrnvav
      121 glllnflidr pslhkealqq divpiicsil eidgatggea amhallilgi sndanivfld
      181 erltrilvdi lgtdassels emclelfhgq aedekaklll anagvcelll kllekhspyc
      241 tdeetrcvlk iacnlivlvl tgddsmnsly dnsngivykk lvewlesedr dlqitavlam
      301 gnfartdahc klmvtqnvhc tllkllqdnn tdtadirfqh allsalrnlv ipvdnkavil
      361 kdgllnilyp minissypvv fkllgtlriv idgqseaaie lgkkdglvek vvewceveeh
      421 pgvqgeanrl iawlinnsrd kgitvsviey gaikhlvkml taqhvlmqne alisltittt
      481 isleeceksl leadvgeklc kffnpqhnle ipilhnvlsl lvnivnsdql rkhlgktnli
      541 tlcretirvk edsslqekle kicdkii
//
LOCUS       XP_034184726             582 aa            linear   INV 03-SEP-2025
DEFINITION  calnexin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184726
VERSION     XP_034184726.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328835.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..582
                     /product="calnexin isoform X1"
                     /calculated_mol_wt=67162
     Region          51..423
                     /region_name="Calreticulin"
                     /note="Calreticulin family; pfam00262"
                     /db_xref="CDD:459737"
     CDS             1..582
                     /gene="LOC117606428"
                     /coded_by="XM_034328835.2:1..1749"
                     /db_xref="GeneID:117606428"
ORIGIN      
        1 mariianiii ylclvcsiis anedndnive nvrsetvykt pevplvetsy lvetfndeek
       61 fknrwvlsea kkdsidedia kydgiwsvee pkkhaqegdl glvlkskarh aaiaaelsep
      121 fylkdkplvv qyevnfqegq ecggaylkll tlaqghedlk kfhdktpyti mfgpdkcgnd
      181 hklhfifrhk nplngsieek hckkprerle dffkdkqphl ytlivrpdns feikvdnkvv
      241 nsgsllddft ppvnppleie dptdvqpedw ddrekipdls avkpddwded apaqivdedd
      301 vmpkgwlede ppmipnpdsv kpedwdvemd gdweppeipn pkcadvpgcg pykqkmkknp
      361 rykgrwsppl innpnykgkw kprlihnpdy fndehpfrmm pifaigfelw smstdiffdn
      421 ilitddeeva akwaeetfev rrariaeesv tlwgrilkat nykpgwwaly flycaipvvl
      481 yvwyllkrlr edkydlkded kqplevneet eskqeenenp ppikepegeg eggeeeeeee
      541 egggegeeeg kqneieemie seendkpavg gdgprrrkpn ke
//
LOCUS       XP_076545728             581 aa            linear   INV 03-SEP-2025
DEFINITION  calnexin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545728
VERSION     XP_076545728.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689613.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="calnexin isoform X2"
                     /calculated_mol_wt=66727
     Region          51..423
                     /region_name="Calreticulin"
                     /note="Calreticulin family; pfam00262"
                     /db_xref="CDD:459737"
     CDS             1..581
                     /gene="LOC117606428"
                     /coded_by="XM_076689613.1:1..1746"
                     /db_xref="GeneID:117606428"
ORIGIN      
        1 mariianiii ylclvcsiis anedndnive nvrsetvykt pevplvetsy lvetfndeek
       61 fknrwvlsea kkdsidedia kydgiwsvee pkkhaqegdl glvlkskarh aaiaaelsep
      121 fylkdkplvv qyevnfqegq ecggaylkll tlaqghedlk kfhdktpyti mfgpdkcgnd
      181 hklhfifrhk nplngsieek hckkprerle dffkdkqphl ytlivrpdns feikvdnkvv
      241 nsgsllddft ppvnppleie dptdvqpedw ddrekipdls avkpddwded apaqivdedd
      301 vmpkgwlede ppmipnpdsv kpedwdvemd gdweppeipn pkcadvpgcg pykqkmkknp
      361 rykgrwsppl innpnykgkw kprlihnpdy fndehpfrmm pifaigfelw smstdiffdn
      421 ilitddeeva akwaeetfev rrariaeesw siwrqigtft aehpwiwavy iiaaglpill
      481 viycccfasq dkydlkdedk qplevneete skqeenenpp pikepegege ggeeeeeeee
      541 gggegeeegk qneieemies eendkpavgg dgprrrkpnk e
//
LOCUS       XP_076545729             540 aa            linear   INV 03-SEP-2025
DEFINITION  calnexin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545729
VERSION     XP_076545729.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689614.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="calnexin isoform X3"
                     /calculated_mol_wt=62003
     Region          51..423
                     /region_name="Calreticulin"
                     /note="Calreticulin family; pfam00262"
                     /db_xref="CDD:459737"
     CDS             1..540
                     /gene="LOC117606428"
                     /coded_by="XM_076689614.1:1..1623"
                     /db_xref="GeneID:117606428"
ORIGIN      
        1 mariianiii ylclvcsiis anedndnive nvrsetvykt pevplvetsy lvetfndeek
       61 fknrwvlsea kkdsidedia kydgiwsvee pkkhaqegdl glvlkskarh aaiaaelsep
      121 fylkdkplvv qyevnfqegq ecggaylkll tlaqghedlk kfhdktpyti mfgpdkcgnd
      181 hklhfifrhk nplngsieek hckkprerle dffkdkqphl ytlivrpdns feikvdnkvv
      241 nsgsllddft ppvnppleie dptdvqpedw ddrekipdls avkpddwded apaqivdedd
      301 vmpkgwlede ppmipnpdsv kpedwdvemd gdweppeipn pkcadvpgcg pykqkmkknp
      361 rykgrwsppl innpnykgkw kprlihnpdy fndehpfrmm pifaigfelw smstdiffdn
      421 ilitddeeva akwaeetfev rrariaeesd kydlkdedkq plevneetes kqeenenppp
      481 ikepegegeg geeeeeeeeg ggegeeegkq neieemiese endkpavggd gprrrkpnke
//
LOCUS       XP_034184744             533 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606433 [Osmia lignaria lignaria].
ACCESSION   XP_034184744
VERSION     XP_034184744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="uncharacterized protein LOC117606433"
                     /calculated_mol_wt=59516
     Region          <224..457
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          274..346
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..533
                     /gene="LOC117606433"
                     /coded_by="XM_034328853.2:757..2358"
                     /db_xref="GeneID:117606433"
ORIGIN      
        1 migpssgtgg vsehevsdnq srsgkkrife dldienfcee vqnsrkryqy qeietpmemv
       61 asnqgisdta affsplssqe vvdecpvarl evllvdgtny twttvseles qqnvtevats
      121 psassssseh rqeqviqevq ieemsypvgs dywesvvtvp slnlpqnkig gscngqqqqq
      181 qfddqetgnl swlldfkldp fieaadekst atparnnyig nkakingrsy gslcsndvkr
      241 synengsshq dlnqsysnfd nrnfassrcn gpkkppftyt eliehalrer geltvsaiyq
      301 wisehfpyyk snddrwknsv rhnlsinphf rkgskaphga ghlwaianrs gdsrprqtin
      361 tsssikqvvk saieaennwk nisqinpide veaatasiiq qpseeeteni vnsvtlehca
      421 eeilsgikke vevqylvpmm vsnnehnstq sdqqtqelhy pvkesdflnp vskevvaeec
      481 glisegylvt dlnpttlgln ivepeiitpe nlfgeelsfq fyelsspsqi qsa
//
LOCUS       XP_034184745             533 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606433 [Osmia lignaria lignaria].
ACCESSION   XP_034184745
VERSION     XP_034184745.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328854.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="uncharacterized protein LOC117606433"
                     /calculated_mol_wt=59516
     Region          <224..457
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          274..346
                     /region_name="Forkhead"
                     /note="Forkhead domain; pfam00250"
                     /db_xref="CDD:459732"
     CDS             1..533
                     /gene="LOC117606433"
                     /coded_by="XM_034328854.2:537..2138"
                     /db_xref="GeneID:117606433"
ORIGIN      
        1 migpssgtgg vsehevsdnq srsgkkrife dldienfcee vqnsrkryqy qeietpmemv
       61 asnqgisdta affsplssqe vvdecpvarl evllvdgtny twttvseles qqnvtevats
      121 psassssseh rqeqviqevq ieemsypvgs dywesvvtvp slnlpqnkig gscngqqqqq
      181 qfddqetgnl swlldfkldp fieaadekst atparnnyig nkakingrsy gslcsndvkr
      241 synengsshq dlnqsysnfd nrnfassrcn gpkkppftyt eliehalrer geltvsaiyq
      301 wisehfpyyk snddrwknsv rhnlsinphf rkgskaphga ghlwaianrs gdsrprqtin
      361 tsssikqvvk saieaennwk nisqinpide veaatasiiq qpseeeteni vnsvtlehca
      421 eeilsgikke vevqylvpmm vsnnehnstq sdqqtqelhy pvkesdflnp vskevvaeec
      481 glisegylvt dlnpttlgln ivepeiitpe nlfgeelsfq fyelsspsqi qsa
//
LOCUS       XP_034184749             475 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA methyltransferase 6 non-catalytic subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034184749
VERSION     XP_034184749.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328858.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184749.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="tRNA methyltransferase 6 non-catalytic subunit"
                     /calculated_mol_wt=54302
     Region          13..271
                     /region_name="Gcd10p"
                     /note="Gcd10p family; pfam04189"
                     /db_xref="CDD:461216"
     Region          <284..>359
                     /region_name="auto_Ata"
                     /note="trimeric autotransporter adhesin Ata; NF033481"
                     /db_xref="CDD:411124"
     CDS             1..475
                     /gene="Trmt6"
                     /coded_by="XM_034328858.2:1..1428"
                     /db_xref="GeneID:117606435"
ORIGIN      
        1 mklknmcese dniitegkyv ivkkqnfkki ykvttngnlt lgrdlvdmsq iigkrfwttf
       61 emipakhgkr thtlkettet eslndllnel psgndnrtin ddgtsqklsk eeilqlqesg
      121 ksgkeivgsl iennkcfldr tgysqekylk kkekkylryl tvckpnissl hdvyfqldhs
      181 kianlrmdtl aqllsysdvq seglyilyds gsqglpaaam lnrigtnteg clinlhpgnv
      241 hqamlinsmn fpveqlnrlv nvniysflrl hyqgestlld niskkihnrk nkskeneskn
      301 sknmltdnei lkdektdien clentnksse iikeedtdsn lttlkrklde snedesteii
      361 pmkkpkwffe tqravelvqn skarglviva kehplniata llsflgasrp fviyhvhrep
      421 lqetymmlkq kqnvinlrln snflrsyqvl pdrthpdilt sdtggylltg ylvqq
//
LOCUS       XP_034184754             467 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2B subunit gamma [Osmia
            lignaria lignaria].
ACCESSION   XP_034184754
VERSION     XP_034184754.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328863.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184754.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="eukaryotic translation initiation factor 2B
                     subunit gamma"
                     /calculated_mol_wt=52424
     Region          7..219
                     /region_name="eIF-2B_gamma_N"
                     /note="The N-terminal domain of gamma subunit of the
                     eIF-2B is a subfamily of glycosyltransferase 2; cd04198"
                     /db_xref="CDD:133041"
     Region          373..451
                     /region_name="LbetaH"
                     /note="Left-handed parallel beta-Helix (LbetaH or LbH)
                     domain: The alignment contains 5 turns, each containing
                     three imperfect tandem repeats of a hexapeptide repeat
                     motif (X-[STAV]-X-[LIV]-[GAED]-X). Proteins containing
                     hexapeptide repeats are often enzymes...; cl00160"
                     /db_xref="CDD:469633"
     Site            order(384,386,389,391,401,406,408,418,422..423,425,435,
                     440,442,444)
                     /site_type="other"
                     /note="putative trimer interface [polypeptide binding]"
                     /db_xref="CDD:100038"
     Site            order(418,420..421,425,437,444)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:100038"
     CDS             1..467
                     /gene="eIF2Bgamma"
                     /coded_by="XM_034328863.2:1..1404"
                     /db_xref="GeneID:117606436"
ORIGIN      
        1 mfpykefqai vlaggggsrm telthgqhkc llpignvpmv wyplqlleht gfkeaivvvs
       61 enmennvsla inklnlkikv divsvedaee lgtadsirli hekiytdflv iscdlitdvd
      121 isevlnlyrk hnasitalml ptpkvpddfv tpgpknkqkp etdligidne tgrliflasg
      181 sdfeetinis qtlikkhpsf tlysklmdah lyiiskwvld llmhnknltt lkgellpyiv
      241 skqfskppnk qcaddkntsi vqvdlkediy rfavekslde likkmsaynd hstdledayn
      301 gdiircyayi gngkfglran tiqmyhfint kifewwnadn syqhpyfrse qsllmkispt
      361 aivhstqiqg csvddnalin ektslkeshi gpnvlveskt rvsssiimen vtikqrcaih
      421 ncilcngcvi eegtelkdci vgpqhtviag sqhsrevltd pdkliei
//
LOCUS       XP_034184763             410 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase (ubiquinone) subunit ND-42 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184763
VERSION     XP_034184763.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328872.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184763.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="NADH dehydrogenase (ubiquinone) subunit ND-42"
                     /calculated_mol_wt=47849
     Region          78..302
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     Site            order(83,88..91)
                     /site_type="active"
                     /db_xref="CDD:238977"
     CDS             1..410
                     /gene="ND-42"
                     /coded_by="XM_034328872.2:147..1379"
                     /db_xref="GeneID:117606439"
ORIGIN      
        1 masvlsigvs kitpaaflpr lckipknhna iqvafmkrla lcdpvrkpap fkyweklysa
       61 fqmaktsivm rydentriiv vdgppavgkt klceklaeef gmmympppgh delyinpygy
      121 dlremdsklp lhaqsydler flakpndvrv pffqlmyylm rfeqyanail hvlstgqgvv
      181 lnrcaysdia fteamlksgy iskeamsqym fmknkgckkl frphlviyld vppelvkeki
      241 kkrgnphevn skvfttkfls dlenaykeky lktlkehsql liydwskegn fndvihdvee
      301 vdldtfkkpr ladwifgsva eiydaiqifq ekvpivldmy nnmevpprel ywtaeefdli
      361 estmnsipte kydygffpki dknlwfkydq pelhpfrrtp rdfvnlnksm
//
LOCUS       XP_034184762             410 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase (ubiquinone) subunit ND-42 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184762
VERSION     XP_034184762.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328871.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184762.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="NADH dehydrogenase (ubiquinone) subunit ND-42"
                     /calculated_mol_wt=47849
     Region          78..302
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     Site            order(83,88..91)
                     /site_type="active"
                     /db_xref="CDD:238977"
     CDS             1..410
                     /gene="ND-42"
                     /coded_by="XM_034328871.2:88..1320"
                     /db_xref="GeneID:117606439"
ORIGIN      
        1 masvlsigvs kitpaaflpr lckipknhna iqvafmkrla lcdpvrkpap fkyweklysa
       61 fqmaktsivm rydentriiv vdgppavgkt klceklaeef gmmympppgh delyinpygy
      121 dlremdsklp lhaqsydler flakpndvrv pffqlmyylm rfeqyanail hvlstgqgvv
      181 lnrcaysdia fteamlksgy iskeamsqym fmknkgckkl frphlviyld vppelvkeki
      241 kkrgnphevn skvfttkfls dlenaykeky lktlkehsql liydwskegn fndvihdvee
      301 vdldtfkkpr ladwifgsva eiydaiqifq ekvpivldmy nnmevpprel ywtaeefdli
      361 estmnsipte kydygffpki dknlwfkydq pelhpfrrtp rdfvnlnksm
//
LOCUS       XP_034184766             364 aa            linear   INV 03-SEP-2025
DEFINITION  testis-specific serine/threonine-protein kinase 6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184766
VERSION     XP_034184766.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328875.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="testis-specific serine/threonine-protein kinase
                     6"
                     /calculated_mol_wt=41437
     Region          18..284
                     /region_name="STKc_TSSK-like"
                     /note="Catalytic domain of testis-specific
                     serine/threonine kinases and similar proteins; cd14080"
                     /db_xref="CDD:270982"
     Site            order(25..29,33,51,53,85,101..104,108,110..111,113,
                     146..147,149,151..152,154,164..165,168,186..190,192,
                     222..225)
                     /site_type="active"
                     /db_xref="CDD:270982"
     Site            order(25..28,33,51,53,85,101..104,108,151..152,154,
                     164..165)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270982"
     Site            order(108,110..111,113,147,149,151,168,186..190,192,
                     222..225)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270982"
     Site            order(164..175,181..190)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270982"
     CDS             1..364
                     /gene="LOC117606440"
                     /coded_by="XM_034328875.2:463..1557"
                     /db_xref="GeneID:117606440"
ORIGIN      
        1 mtdlnqtpse eavllargyk fvkklgegsy akvylaeykp etepercstl ackvintvna
       61 pkdfvrkflp reldilakln hphvvhvhsi fqrrskyfif mryaengdll dfvlkngava
      121 egqarvwlrq lalglqylhe meiahrdmkc envlltsnhn vkladfgfar yvidnrgkri
      181 lsdtycgsls yaapeilras pynpkmadvw slgvilyill nkampfddtd ikslyeqqtn
      241 rkwkfrskva gslsehvkkl vthllepdph krwkltqvin sdwiamdprl lvltpaeqia
      301 lnnaieekkk legkfiqtdp klfkveekkt ldadtaktka evtvikkaak avmssittgs
      361 ifkk
//
LOCUS       XP_076545730             364 aa            linear   INV 03-SEP-2025
DEFINITION  testis-specific serine/threonine-protein kinase 6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545730
VERSION     XP_076545730.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689615.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="testis-specific serine/threonine-protein kinase
                     6"
                     /calculated_mol_wt=41437
     Region          18..284
                     /region_name="STKc_TSSK-like"
                     /note="Catalytic domain of testis-specific
                     serine/threonine kinases and similar proteins; cd14080"
                     /db_xref="CDD:270982"
     Site            order(25..29,33,51,53,85,101..104,108,110..111,113,
                     146..147,149,151..152,154,164..165,168,186..190,192,
                     222..225)
                     /site_type="active"
                     /db_xref="CDD:270982"
     Site            order(25..28,33,51,53,85,101..104,108,151..152,154,
                     164..165)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270982"
     Site            order(108,110..111,113,147,149,151,168,186..190,192,
                     222..225)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270982"
     Site            order(164..175,181..190)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270982"
     CDS             1..364
                     /gene="LOC117606440"
                     /coded_by="XM_076689615.1:443..1537"
                     /db_xref="GeneID:117606440"
ORIGIN      
        1 mtdlnqtpse eavllargyk fvkklgegsy akvylaeykp etepercstl ackvintvna
       61 pkdfvrkflp reldilakln hphvvhvhsi fqrrskyfif mryaengdll dfvlkngava
      121 egqarvwlrq lalglqylhe meiahrdmkc envlltsnhn vkladfgfar yvidnrgkri
      181 lsdtycgsls yaapeilras pynpkmadvw slgvilyill nkampfddtd ikslyeqqtn
      241 rkwkfrskva gslsehvkkl vthllepdph krwkltqvin sdwiamdprl lvltpaeqia
      301 lnnaieekkk legkfiqtdp klfkveekkt ldadtaktka evtvikkaak avmssittgs
      361 ifkk
//
LOCUS       XP_034184769             337 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,3-galactosyltransferase 6 [Osmia lignaria lignaria].
ACCESSION   XP_034184769
VERSION     XP_034184769.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328878.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="beta-1,3-galactosyltransferase 6"
                     /calculated_mol_wt=39251
     Region          79..267
                     /region_name="Galactosyl_T"
                     /note="Galactosyltransferase; cl21608"
                     /db_xref="CDD:473923"
     CDS             1..337
                     /gene="beta3GalTII"
                     /coded_by="XM_034328878.2:1..1014"
                     /db_xref="GeneID:117606442"
ORIGIN      
        1 mdsvtllplr pssknlymmi rkklhvklni ptlliivmvv flcihylsat rcsqndkeik
       61 akskfrliil ilsspdnleq ratirktwlp qkqatvkhlf vigtldllae qketlqsekq
      121 kfndllllpr ltdsygtltk kllyalkety knydfnfllk cdddtfvlvh kllkeldkwe
      181 skgtrkelyw gffngkaqvk rsgpwketdw vlcdyylpya lgggyvlsyn lvkfiasnmd
      241 ilklhkaedv svglwlapla nierkhdvrf dteyrsrgcs nqyivthkqt ienmknlhey
      301 yqasgalcmk eiryrmsyhy nwtvppsqcc nlqsgip
//
LOCUS       XP_034184771             336 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 16 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184771
VERSION     XP_034184771.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328880.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184771.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="peroxisomal biogenesis factor 16 isoform X1"
                     /calculated_mol_wt=39034
     Region          15..328
                     /region_name="Pex16"
                     /note="Peroxisomal membrane protein (Pex16); pfam08610"
                     /db_xref="CDD:462535"
     CDS             1..336
                     /gene="Pex16"
                     /coded_by="XM_034328880.2:196..1206"
                     /db_xref="GeneID:117606443"
ORIGIN      
        1 mvfdtvnssv lkviepyrrw iienpqllad ventvqclsy ftagqfnnst fvsellysls
       61 nlivlfndll mcsgkcmrlk fpqfeskiki wltvveytet lfeitakklw gqtgkwyiit
      121 iiqifktvlr lllvhlyker itksppiqpl nreklnesdd dklkegfklk rcgtvvrsir
      181 gtnnapmriw eplsstisdn dnlncssase knlllaesly ivkpllhlgc isltgekqwl
      241 pwllsfaids lslkifsnea qnmlfskeee keifrrrlal llyilkspfy drysciriya
      301 iltalsnkvp lakfvterik tylphwqsty fytwss
//
LOCUS       XP_076545731             336 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 16 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545731
VERSION     XP_076545731.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689616.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="peroxisomal biogenesis factor 16 isoform X1"
                     /calculated_mol_wt=39034
     Region          15..328
                     /region_name="Pex16"
                     /note="Peroxisomal membrane protein (Pex16); pfam08610"
                     /db_xref="CDD:462535"
     CDS             1..336
                     /gene="Pex16"
                     /coded_by="XM_076689616.1:202..1212"
                     /db_xref="GeneID:117606443"
ORIGIN      
        1 mvfdtvnssv lkviepyrrw iienpqllad ventvqclsy ftagqfnnst fvsellysls
       61 nlivlfndll mcsgkcmrlk fpqfeskiki wltvveytet lfeitakklw gqtgkwyiit
      121 iiqifktvlr lllvhlyker itksppiqpl nreklnesdd dklkegfklk rcgtvvrsir
      181 gtnnapmriw eplsstisdn dnlncssase knlllaesly ivkpllhlgc isltgekqwl
      241 pwllsfaids lslkifsnea qnmlfskeee keifrrrlal llyilkspfy drysciriya
      301 iltalsnkvp lakfvterik tylphwqsty fytwss
//
LOCUS       XP_034184772             336 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 16 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184772
VERSION     XP_034184772.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328881.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184772.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="peroxisomal biogenesis factor 16 isoform X1"
                     /calculated_mol_wt=39034
     Region          15..328
                     /region_name="Pex16"
                     /note="Peroxisomal membrane protein (Pex16); pfam08610"
                     /db_xref="CDD:462535"
     CDS             1..336
                     /gene="Pex16"
                     /coded_by="XM_034328881.2:198..1208"
                     /db_xref="GeneID:117606443"
ORIGIN      
        1 mvfdtvnssv lkviepyrrw iienpqllad ventvqclsy ftagqfnnst fvsellysls
       61 nlivlfndll mcsgkcmrlk fpqfeskiki wltvveytet lfeitakklw gqtgkwyiit
      121 iiqifktvlr lllvhlyker itksppiqpl nreklnesdd dklkegfklk rcgtvvrsir
      181 gtnnapmriw eplsstisdn dnlncssase knlllaesly ivkpllhlgc isltgekqwl
      241 pwllsfaids lslkifsnea qnmlfskeee keifrrrlal llyilkspfy drysciriya
      301 iltalsnkvp lakfvterik tylphwqsty fytwss
//
LOCUS       XP_034184773             326 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 16 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184773
VERSION     XP_034184773.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328882.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184773.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="peroxisomal biogenesis factor 16 isoform X2"
                     /calculated_mol_wt=37800
     Region          15..318
                     /region_name="Pex16"
                     /note="Peroxisomal membrane protein (Pex16); pfam08610"
                     /db_xref="CDD:462535"
     CDS             1..326
                     /gene="Pex16"
                     /coded_by="XM_034328882.2:178..1158"
                     /db_xref="GeneID:117606443"
ORIGIN      
        1 mvfdtvnssv lkviepyrrw iienpqllad ventvqclsy ftagqfnnst fvsellysls
       61 nlivlfndll mcsgkcmrlk fpqfeskiki wltvveytet lfeitakklw gqtgkwtvlr
      121 lllvhlyker itksppiqpl nreklnesdd dklkegfklk rcgtvvrsir gtnnapmriw
      181 eplsstisdn dnlncssase knlllaesly ivkpllhlgc isltgekqwl pwllsfaids
      241 lslkifsnea qnmlfskeee keifrrrlal llyilkspfy drysciriya iltalsnkvp
      301 lakfvterik tylphwqsty fytwss
//
LOCUS       XP_076545732             311 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 16 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545732
VERSION     XP_076545732.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689617.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="peroxisomal biogenesis factor 16 isoform X3"
                     /calculated_mol_wt=36297
     Region          16..303
                     /region_name="Pex16"
                     /note="Peroxisomal membrane protein (Pex16); pfam08610"
                     /db_xref="CDD:462535"
     CDS             1..311
                     /gene="Pex16"
                     /coded_by="XM_076689617.1:265..1200"
                     /db_xref="GeneID:117606443"
ORIGIN      
        1 mwktqynvyl ilqlnillgq fnnstfvsel lyslsnlivl fndllmcsgk cmrlkfpqfe
       61 skikiwltvv eytetlfeit akklwgqtgk wyiitiiqif ktvlrlllvh lykeritksp
      121 piqplnrekl nesdddklke gfklkrcgtv vrsirgtnna pmriweplss tisdndnlnc
      181 ssaseknlll aeslyivkpl lhlgcisltg ekqwlpwlls faidslslki fsneaqnmlf
      241 skeeekeifr rrlalllyil kspfydrysc iriyailtal snkvplakfv teriktylph
      301 wqstyfytws s
//
LOCUS       XP_034184774             329 aa            linear   INV 03-SEP-2025
DEFINITION  thymidylate synthase [Osmia lignaria lignaria].
ACCESSION   XP_034184774
VERSION     XP_034184774.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328883.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184774.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="thymidylate synthase"
                     /calculated_mol_wt=37639
     Region          <16..329
                     /region_name="PTZ00164"
                     /note="bifunctional dihydrofolate reductase-thymidylate
                     synthase; Provisional"
                     /db_xref="CDD:240299"
     CDS             1..329
                     /gene="Ts"
                     /coded_by="XM_034328883.2:1..990"
                     /db_xref="GeneID:117606444"
ORIGIN      
        1 msenkvisln qnvahdmidk iiemngngng kksevtrvei eeheeyqyln likrviktga
       61 ekndrtsigt lsifgthmrf nlrnnvfpll ttkrvfwkgv veellwfikg stnakelsek
      121 nvhiwdanss rayldscgfn dreegdlgpv ygfqwrhyga eykdmhanye gqgidqlkev
      181 iekikhtpnd rriiisawnp ldipkmalpp chcfvqfyvs ngelsshlyq rsadmglgvp
      241 fniasysllt ymlahitnlk pgefvhtmgd chvylnhisa leeqikrvpk pfpqlkivrn
      301 vetiddfiae dfkligyeph akipmpmav
//
LOCUS       XP_034184775             291 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 6 [Osmia lignaria lignaria].
ACCESSION   XP_034184775
VERSION     XP_034184775.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328884.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184775.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="very long chain fatty acid elongase 6"
                     /calculated_mol_wt=34486
     Region          32..272
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..291
                     /gene="LOC117606445"
                     /coded_by="XM_034328884.2:346..1221"
                     /db_xref="GeneID:117606445"
ORIGIN      
        1 mnkvdymevt lpnysyvfnf eetfspaetq qwmiknwtng fyycgiymil ifggqhymsn
       61 rpkfelrgll tvwntvlaif siigftrtap eliyvlrnyg fhysicvpsf ierncvsgfw
      121 twmfvlsklp elgdtifivl rkqrliflhw yhhitvllya wfsyteyaat arwyivmnyf
      181 vhsimysyya lkamryrppq wiamlitslq ilqmvmgviv nirafqyles gkidchisrv
      241 nvtlglliyf syfilfakff rssylnasrg tkvakksyvn gteeyeklka n
//
LOCUS       XP_034184776             291 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 6 [Osmia lignaria lignaria].
ACCESSION   XP_034184776
VERSION     XP_034184776.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328885.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184776.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="very long chain fatty acid elongase 6"
                     /calculated_mol_wt=34486
     Region          32..272
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..291
                     /gene="LOC117606445"
                     /coded_by="XM_034328885.2:664..1539"
                     /db_xref="GeneID:117606445"
ORIGIN      
        1 mnkvdymevt lpnysyvfnf eetfspaetq qwmiknwtng fyycgiymil ifggqhymsn
       61 rpkfelrgll tvwntvlaif siigftrtap eliyvlrnyg fhysicvpsf ierncvsgfw
      121 twmfvlsklp elgdtifivl rkqrliflhw yhhitvllya wfsyteyaat arwyivmnyf
      181 vhsimysyya lkamryrppq wiamlitslq ilqmvmgviv nirafqyles gkidchisrv
      241 nvtlglliyf syfilfakff rssylnasrg tkvakksyvn gteeyeklka n
//
LOCUS       XP_034184778             291 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 6 [Osmia lignaria lignaria].
ACCESSION   XP_034184778
VERSION     XP_034184778.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328887.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184778.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="very long chain fatty acid elongase 6"
                     /calculated_mol_wt=34486
     Region          32..272
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..291
                     /gene="LOC117606445"
                     /coded_by="XM_034328887.2:610..1485"
                     /db_xref="GeneID:117606445"
ORIGIN      
        1 mnkvdymevt lpnysyvfnf eetfspaetq qwmiknwtng fyycgiymil ifggqhymsn
       61 rpkfelrgll tvwntvlaif siigftrtap eliyvlrnyg fhysicvpsf ierncvsgfw
      121 twmfvlsklp elgdtifivl rkqrliflhw yhhitvllya wfsyteyaat arwyivmnyf
      181 vhsimysyya lkamryrppq wiamlitslq ilqmvmgviv nirafqyles gkidchisrv
      241 nvtlglliyf syfilfakff rssylnasrg tkvakksyvn gteeyeklka n
//
LOCUS       XP_034184777             291 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 6 [Osmia lignaria lignaria].
ACCESSION   XP_034184777
VERSION     XP_034184777.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328886.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184777.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="very long chain fatty acid elongase 6"
                     /calculated_mol_wt=34486
     Region          32..272
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..291
                     /gene="LOC117606445"
                     /coded_by="XM_034328886.2:423..1298"
                     /db_xref="GeneID:117606445"
ORIGIN      
        1 mnkvdymevt lpnysyvfnf eetfspaetq qwmiknwtng fyycgiymil ifggqhymsn
       61 rpkfelrgll tvwntvlaif siigftrtap eliyvlrnyg fhysicvpsf ierncvsgfw
      121 twmfvlsklp elgdtifivl rkqrliflhw yhhitvllya wfsyteyaat arwyivmnyf
      181 vhsimysyya lkamryrppq wiamlitslq ilqmvmgviv nirafqyles gkidchisrv
      241 nvtlglliyf syfilfakff rssylnasrg tkvakksyvn gteeyeklka n
//
LOCUS       XP_034184779             291 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 6 [Osmia lignaria lignaria].
ACCESSION   XP_034184779
VERSION     XP_034184779.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328888.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184779.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="very long chain fatty acid elongase 6"
                     /calculated_mol_wt=34486
     Region          32..272
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..291
                     /gene="LOC117606445"
                     /coded_by="XM_034328888.2:747..1622"
                     /db_xref="GeneID:117606445"
ORIGIN      
        1 mnkvdymevt lpnysyvfnf eetfspaetq qwmiknwtng fyycgiymil ifggqhymsn
       61 rpkfelrgll tvwntvlaif siigftrtap eliyvlrnyg fhysicvpsf ierncvsgfw
      121 twmfvlsklp elgdtifivl rkqrliflhw yhhitvllya wfsyteyaat arwyivmnyf
      181 vhsimysyya lkamryrppq wiamlitslq ilqmvmgviv nirafqyles gkidchisrv
      241 nvtlglliyf syfilfakff rssylnasrg tkvakksyvn gteeyeklka n
//
LOCUS       XP_076545733             286 aa            linear   INV 03-SEP-2025
DEFINITION  RWD domain-containing protein 2A [Osmia lignaria lignaria].
ACCESSION   XP_076545733
VERSION     XP_076545733.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689618.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="RWD domain-containing protein 2A"
                     /calculated_mol_wt=33062
     Region          10..133
                     /region_name="RWD_RWDD2"
                     /note="RWD domain of RWD domain-containing protein 2A
                     (RWDD2A), 2B (RWDD2B) and related proteins; cd23829"
                     /db_xref="CDD:467663"
     Region          158..280
                     /region_name="RWDD2_C"
                     /note="C-terminal domain of RWD domain-containing protein
                     2; cd24163"
                     /db_xref="CDD:467814"
     CDS             1..286
                     /gene="LOC143305561"
                     /coded_by="XM_076689618.1:105..965"
                     /db_xref="GeneID:143305561"
ORIGIN      
        1 mctheeisen ltaqvcelet lqsvypkeiv itdhgvladi hdflknptqd fphrleysve
       61 islsngtiel lislppnypn dkpeiyarns llnriqqlql nkalknvven qepgepciyv
      121 lilwlqdnge dylvasnenq ekeltdiskn keeksitfar ywiyshhiyg kskrkdiidl
      181 akensitgfc lagkpgiicm egvfedcdyc wqkiksmnwq kilirlleke enhsgidsir
      241 kfsdfreisf psserhndmg qvlkylkehe vqyafkelfg iegktt
//
LOCUS       XP_034184783             282 aa            linear   INV 03-SEP-2025
DEFINITION  toll-interacting protein B [Osmia lignaria lignaria].
ACCESSION   XP_034184783
VERSION     XP_034184783.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328892.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="toll-interacting protein B"
                     /calculated_mol_wt=31842
     Region          65..186
                     /region_name="C2_Tollip"
                     /note="C2 domain present in Toll-interacting protein
                     (Tollip); cd04016"
                     /db_xref="CDD:175983"
     Site            order(87,134,136,142)
                     /site_type="other"
                     /note="putative Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:175983"
     Region          241..280
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     CDS             1..282
                     /gene="LOC117606446"
                     /coded_by="XM_034328892.2:183..1031"
                     /db_xref="GeneID:117606446"
ORIGIN      
        1 meterrsdly eewkrraflg plpqgflrle ernaqqqqea adqqaalalh qlqmqnmpam
       61 hephvgrlsi tivqaklvkn ygmtrmdpyv rlrvghavye thtcsngakn phwnkviqcy
      121 lppgvtqiyi eiydecsfvm deliawghid ippqvlqkge ihedwymlsg kqgdnqegmi
      181 nlvfsyttkc hpymsaappi mmvpsstmfg mtqyapvnvy tapptaaaat pvvpssmpna
      241 evelkqisem fpnvdkevik svydanhgkk dvtinsllqm ce
//
LOCUS       XP_034184781             282 aa            linear   INV 03-SEP-2025
DEFINITION  toll-interacting protein B [Osmia lignaria lignaria].
ACCESSION   XP_034184781
VERSION     XP_034184781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="toll-interacting protein B"
                     /calculated_mol_wt=31842
     Region          65..186
                     /region_name="C2_Tollip"
                     /note="C2 domain present in Toll-interacting protein
                     (Tollip); cd04016"
                     /db_xref="CDD:175983"
     Site            order(87,134,136,142)
                     /site_type="other"
                     /note="putative Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:175983"
     Region          241..280
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     CDS             1..282
                     /gene="LOC117606446"
                     /coded_by="XM_034328890.2:231..1079"
                     /db_xref="GeneID:117606446"
ORIGIN      
        1 meterrsdly eewkrraflg plpqgflrle ernaqqqqea adqqaalalh qlqmqnmpam
       61 hephvgrlsi tivqaklvkn ygmtrmdpyv rlrvghavye thtcsngakn phwnkviqcy
      121 lppgvtqiyi eiydecsfvm deliawghid ippqvlqkge ihedwymlsg kqgdnqegmi
      181 nlvfsyttkc hpymsaappi mmvpsstmfg mtqyapvnvy tapptaaaat pvvpssmpna
      241 evelkqisem fpnvdkevik svydanhgkk dvtinsllqm ce
//
LOCUS       XP_034184782             282 aa            linear   INV 03-SEP-2025
DEFINITION  toll-interacting protein B [Osmia lignaria lignaria].
ACCESSION   XP_034184782
VERSION     XP_034184782.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328891.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="toll-interacting protein B"
                     /calculated_mol_wt=31842
     Region          65..186
                     /region_name="C2_Tollip"
                     /note="C2 domain present in Toll-interacting protein
                     (Tollip); cd04016"
                     /db_xref="CDD:175983"
     Site            order(87,134,136,142)
                     /site_type="other"
                     /note="putative Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:175983"
     Region          241..280
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     CDS             1..282
                     /gene="LOC117606446"
                     /coded_by="XM_034328891.2:227..1075"
                     /db_xref="GeneID:117606446"
ORIGIN      
        1 meterrsdly eewkrraflg plpqgflrle ernaqqqqea adqqaalalh qlqmqnmpam
       61 hephvgrlsi tivqaklvkn ygmtrmdpyv rlrvghavye thtcsngakn phwnkviqcy
      121 lppgvtqiyi eiydecsfvm deliawghid ippqvlqkge ihedwymlsg kqgdnqegmi
      181 nlvfsyttkc hpymsaappi mmvpsstmfg mtqyapvnvy tapptaaaat pvvpssmpna
      241 evelkqisem fpnvdkevik svydanhgkk dvtinsllqm ce
//
LOCUS       XP_034184784             282 aa            linear   INV 03-SEP-2025
DEFINITION  28S ribosomal protein S15, mitochondrial [Osmia lignaria lignaria].
ACCESSION   XP_034184784
VERSION     XP_034184784.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184784.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="28S ribosomal protein S15, mitochondrial"
                     /calculated_mol_wt=33289
     Region          <75..>215
                     /region_name="S15_NS1_EPRS_RNA-bind"
                     /note="S15/NS1/EPRS_RNA-binding domain. This short domain
                     consists of a helix-turn-helix structure, which can bind
                     to several types of RNA. It is found in the ribosomal
                     protein S15, the influenza A viral nonstructural protein
                     (NSA) and in several eukaryotic...; cl00349"
                     /db_xref="CDD:469733"
     CDS             1..282
                     /gene="bonsai"
                     /coded_by="XM_034328893.2:135..983"
                     /db_xref="GeneID:117606447"
ORIGIN      
        1 mnltisrrll ctkmnnicmi ggylsrnfat iddynikwtr perstftdpk fsgdlgldvn
       61 vkptdiklyy ekskeledad dvvkrmfslk fqrnketkrl ksekimhlvk rhvcdrgsme
      121 vsiaamtsei hdlqkyyleq psnkrakvfl keliekrkks lkflrsldyg rfewvleqln
      181 leykllpekp nmisrkeslr mlteeycnni iqekidaykv klkekqkkfy lekaeklali
      241 mkeekecglt ptvteeeied arqkakllnk seqrdsssdi ne
//
LOCUS       XP_034184785             279 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S2 [Osmia lignaria lignaria].
ACCESSION   XP_034184785
VERSION     XP_034184785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..279
                     /product="ribosomal protein S2"
                     /calculated_mol_wt=30073
     Region          44..264
                     /region_name="PTZ00070"
                     /note="40S ribosomal protein S2; Provisional"
                     /db_xref="CDD:240255"
     CDS             1..279
                     /gene="RpS2"
                     /coded_by="XM_034328894.2:147..986"
                     /db_xref="GeneID:117606448"
ORIGIN      
        1 madnapaarg gfrggfgsrg gdrggprgrg rggrgrgrgr grgkedskew ipvtklgrlv
       61 rdgkiesleh iylfslpike yeiidkflgp elkdevlkim pvqkqtragq rtrfkafvai
      121 gdykghiglg vkcskevata irgaiilakl svvpvrrgyw gnkigdphtv pckvtgkcgs
      181 vqvrlipapr gtgivsapvp kkllqmagie dcytsargst ctlgnfakat yaaiaktyay
      241 ltpdlwhdqa lrkapyqefa dflsknhkav ggqrsaevv
//
LOCUS       XP_034184786             275 aa            linear   INV 03-SEP-2025
DEFINITION  SKA complex subunit 1 [Osmia lignaria lignaria].
ACCESSION   XP_034184786
VERSION     XP_034184786.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328895.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="SKA complex subunit 1"
                     /calculated_mol_wt=32379
     Region          34..260
                     /region_name="SKA1_N"
                     /note="Spindle and kinetochore-associated protein 1,
                     N-terminal domain; cl26917"
                     /db_xref="CDD:474868"
     CDS             1..275
                     /gene="LOC117606449"
                     /coded_by="XM_034328895.2:273..1100"
                     /db_xref="GeneID:117606449"
ORIGIN      
        1 matscfleki leqqceklkv leiatifids kkeikeelfe mykqvedmhr dieimknrln
       61 kikeqsnrcv elslllkeld kkiihmekni sseliddyhh senslcmsil sedellkqsl
      121 iitdidteeq mtplkdckki lfnepevcpi ipairedefk tipkyligrq pieivnnlid
      181 tinqvlkaky tflsqgkaha rkqgtmslyl hykkqeldvc tnkeyvyfft asdyekyans
      241 klnktklnlm tvlrhckrlr qytvksdihy viqtk
//
LOCUS       XP_034184787             268 aa            linear   INV 03-SEP-2025
DEFINITION  growth arrest and DNA damage-inducible proteins-interacting protein
            CRIF [Osmia lignaria lignaria].
ACCESSION   XP_034184787
VERSION     XP_034184787.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328896.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="growth arrest and DNA damage-inducible
                     proteins-interacting protein CRIF"
                     /calculated_mol_wt=31446
     Region          53..>199
                     /region_name="CR6_interact"
                     /note="Growth arrest and DNA-damage-inducible
                     proteins-interacting protein 1; pfam10147"
                     /db_xref="CDD:431088"
     CDS             1..268
                     /gene="CRIF"
                     /coded_by="XM_034328896.2:190..996"
                     /db_xref="GeneID:117606450"
ORIGIN      
        1 mslkelffks slqlkiqhvi gkrcfsskpv kdiletveet piyieekdeh agleekrnks
       61 rlnpahrnml hdlkpydesq awyhdsvkyk krilgrygie algrpaglaw ptpeevkein
      121 eyekiafplt iqerlskike ekrlneemim arqqeigkkm anmnkmiadi qekvaekeak
      181 lltaqmtker kieeirrkll aeghvgtdki eeeltqmeka ekkkkkeakk tkllekqkew
      241 amklmreakq egqkdetved kektkedn
//
LOCUS       XP_034184788             267 aa            linear   INV 03-SEP-2025
DEFINITION  inhibitor of growth protein 5 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184788
VERSION     XP_034184788.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328897.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184788.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..267
                     /product="inhibitor of growth protein 5 isoform X1"
                     /calculated_mol_wt=30137
     Region          2..222
                     /region_name="TNG2"
                     /note="Chromatin remodeling protein, contains PhD zinc
                     finger [Chromatin structure and dynamics]; COG5034"
                     /db_xref="CDD:227367"
     Region          13..103
                     /region_name="ING_ING4_5"
                     /note="Inhibitor of growth (ING) domain of inhibitor of
                     growth protein ING4, ING5, and similar proteins; cd16859"
                     /db_xref="CDD:341092"
     Site            order(13,16..18,21,24,27,31,38,41..42,45,48,57..58,60..61,
                     64..65,68,71..72,75,78..79,82,85,88..89,92..93,95..96,
                     99..100,103)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:341092"
     CDS             1..267
                     /gene="Ing5"
                     /coded_by="XM_034328897.2:100..903"
                     /db_xref="GeneID:117606451"
ORIGIN      
        1 mtalylehyl dslehlpiel qrnftlmrdl daraqglmkd idkladdylk nvkkespekk
       61 keqlthiqnl fnkakeygdd kvqlaiqtye lvdkhirrld sdlarfeaei qdkalnssra
      121 qeennaskkg rkklkekekr kkgaagasse desktarkkq kkggsvasas sagavgsgaq
      181 vdstalghpa dvldmpvdpn eptyclchqv sygemigcdn pdvspyymfl qtfynfisii
      241 innflfffsv vsyrmvpfcl crinykt
//
LOCUS       XP_034184789             255 aa            linear   INV 03-SEP-2025
DEFINITION  inhibitor of growth protein 5 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184789
VERSION     XP_034184789.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328898.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="inhibitor of growth protein 5 isoform X2"
                     /calculated_mol_wt=28514
     Region          13..103
                     /region_name="ING_ING4_5"
                     /note="Inhibitor of growth (ING) domain of inhibitor of
                     growth protein ING4, ING5, and similar proteins; cd16859"
                     /db_xref="CDD:341092"
     Site            order(13,16..18,21,24,27,31,38,41..42,45,48,57..58,60..61,
                     64..65,68,71..72,75,78..79,82,85,88..89,92..93,95..96,
                     99..100,103)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:341092"
     Region          204..248
                     /region_name="PHD_ING4_5"
                     /note="PHD finger found in inhibitor of growth protein 4
                     (ING4) and 5 (ING5); cd15586"
                     /db_xref="CDD:277061"
     Site            order(204,211..219,227,230,238..239,241,243)
                     /site_type="other"
                     /note="H3K4me3 binding site [polypeptide binding]"
                     /db_xref="CDD:277061"
     CDS             1..255
                     /gene="Ing5"
                     /coded_by="XM_034328898.2:100..867"
                     /db_xref="GeneID:117606451"
ORIGIN      
        1 mtalylehyl dslehlpiel qrnftlmrdl daraqglmkd idkladdylk nvkkespekk
       61 keqlthiqnl fnkakeygdd kvqlaiqtye lvdkhirrld sdlarfeaei qdkalnssra
      121 qeennaskkg rkklkekekr kkgaagasse desktarkkq kkggsvasas sagavgsgaq
      181 vdstalghpa dvldmpvdpn eptyclchqv sygemigcdn pdcpiewfhf acvglttkpk
      241 gkwycpkctq drkkk
//
LOCUS       XP_034184790             228 aa            linear   INV 03-SEP-2025
DEFINITION  mpv17-like protein 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184790
VERSION     XP_034184790.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328899.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184790.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="mpv17-like protein 2 isoform X1"
                     /calculated_mol_wt=26469
     Region          138..199
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..228
                     /gene="LOC117606453"
                     /coded_by="XM_034328899.2:359..1045"
                     /db_xref="GeneID:117606453"
ORIGIN      
        1 mtvfkklfgk yllitntvsc glmmagadmi qqrrehwkks neylssrscv iaaslddeqe
       61 eefsstavsk antymhnymr trnmtavgll qgpfhhwfym mldkmlpgrt alsvvkkmcl
      121 dqtiasptcl giffvglgvl ehrqikeiye elkmklydtw kvdccfwppt qsvnfffipl
      181 ryrvlytnfm tmiydiflsy ikyvstifla csdtifllqf inftgcsi
//
LOCUS       XP_034184791             209 aa            linear   INV 03-SEP-2025
DEFINITION  mpv17-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184791
VERSION     XP_034184791.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328900.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184791.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="mpv17-like protein 2 isoform X2"
                     /calculated_mol_wt=24454
     Region          138..199
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..209
                     /gene="LOC117606453"
                     /coded_by="XM_034328900.2:359..988"
                     /db_xref="GeneID:117606453"
ORIGIN      
        1 mtvfkklfgk yllitntvsc glmmagadmi qqrrehwkks neylssrscv iaaslddeqe
       61 eefsstavsk antymhnymr trnmtavgll qgpfhhwfym mldkmlpgrt alsvvkkmcl
      121 dqtiasptcl giffvglgvl ehrqikeiye elkmklydtw kvdccfwppt qsvnfffipl
      181 ryrvlytnfm tmiydiflsy ikydaqydq
//
LOCUS       XP_034184793             226 aa            linear   INV 03-SEP-2025
DEFINITION  protein ILRUN [Osmia lignaria lignaria].
ACCESSION   XP_034184793
VERSION     XP_034184793.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328902.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..226
                     /product="protein ILRUN"
                     /calculated_mol_wt=24932
     Region          22..64
                     /region_name="UBA_CF106"
                     /note="UBA-like domain found in uncharacterized protein
                     C6orf106 and similar proteins; cd14349"
                     /db_xref="CDD:270534"
     Region          83..181
                     /region_name="N_BRCA1_IG"
                     /note="Ig-like domain from next to BRCA1 gene; pfam16158"
                     /db_xref="CDD:465035"
     CDS             1..226
                     /gene="LOC117606454"
                     /coded_by="XM_034328902.2:1..681"
                     /db_xref="GeneID:117606454"
ORIGIN      
        1 mnmdndldqh llhqfsclgt tdkedlvkql qrllpgsqln ettatffldm nnwnlqaavc
       61 syldfespvq nkfpcmtlic dstigegeai ppltnfrksw hiqnsgteaw pdgvclqhvg
      121 gvqmgectrv pvpslgpket tevsvnlqsp sncglfrskw rmmvkstetf fgdviwmllv
      181 vsesgtlavt qqlhqlsttt ettndtstdv ttlssdlttl yphgip
//
LOCUS       XP_034184794             218 aa            linear   INV 03-SEP-2025
DEFINITION  YKT6 v-SNARE homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184794
VERSION     XP_034184794.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328903.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..218
                     /product="YKT6 v-SNARE homolog isoform X1"
                     /calculated_mol_wt=24728
     Region          22..214
                     /region_name="SNC1"
                     /note="Synaptobrevin/VAMP-like protein [Intracellular
                     trafficking and secretion]; COG5143"
                     /db_xref="CDD:227472"
     CDS             1..218
                     /gene="Ykt6"
                     /coded_by="XM_034328903.2:95..751"
                     /db_xref="GeneID:117606455"
ORIGIN      
        1 mrflqnersm vvncenqnmv klyalsvlyk nptsattlks aydvesfsff qrgsvkefms
       61 fvsktivert qnssrqsvke gdymchvyvr adnlaavlis dheyprrvah tlitkvmdef
      121 slkypqstwd tlsettidfa qlnvylakyq npneadaltk mqndldetki ilhntleavl
      181 qrgeklddlv skseglsaqs kafyktarkt nsccslap
//
LOCUS       XP_034184795             200 aa            linear   INV 03-SEP-2025
DEFINITION  YKT6 v-SNARE homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184795
VERSION     XP_034184795.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328904.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..200
                     /product="YKT6 v-SNARE homolog isoform X2"
                     /calculated_mol_wt=22534
     Region          4..196
                     /region_name="SNC1"
                     /note="Synaptobrevin/VAMP-like protein [Intracellular
                     trafficking and secretion]; COG5143"
                     /db_xref="CDD:227472"
     CDS             1..200
                     /gene="Ykt6"
                     /coded_by="XM_034328904.2:153..755"
                     /db_xref="GeneID:117606455"
ORIGIN      
        1 mvklyalsvl yknptsattl ksaydvesfs ffqrgsvkef msfvsktive rtqnssrqsv
       61 kegdymchvy vradnlaavl isdheyprrv ahtlitkvmd efslkypqst wdtlsettid
      121 faqlnvylak yqnpneadal tkmqndldet kiilhntlea vlqrgekldd lvskseglsa
      181 qskafyktar ktnsccslap
//
LOCUS       XP_076545734             200 aa            linear   INV 03-SEP-2025
DEFINITION  YKT6 v-SNARE homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545734
VERSION     XP_076545734.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689619.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..200
                     /product="YKT6 v-SNARE homolog isoform X2"
                     /calculated_mol_wt=22534
     Region          4..196
                     /region_name="SNC1"
                     /note="Synaptobrevin/VAMP-like protein [Intracellular
                     trafficking and secretion]; COG5143"
                     /db_xref="CDD:227472"
     CDS             1..200
                     /gene="Ykt6"
                     /coded_by="XM_076689619.1:27..629"
                     /db_xref="GeneID:117606455"
ORIGIN      
        1 mvklyalsvl yknptsattl ksaydvesfs ffqrgsvkef msfvsktive rtqnssrqsv
       61 kegdymchvy vradnlaavl isdheyprrv ahtlitkvmd efslkypqst wdtlsettid
      121 faqlnvylak yqnpneadal tkmqndldet kiilhntlea vlqrgekldd lvskseglsa
      181 qskafyktar ktnsccslap
//
LOCUS       XP_034184796             215 aa            linear   INV 03-SEP-2025
DEFINITION  chromatin modification-related protein EAF6/MEAF6 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184796
VERSION     XP_034184796.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328905.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="chromatin modification-related protein
                     EAF6/MEAF6 isoform X1"
                     /calculated_mol_wt=23381
     Region          12..91
                     /region_name="NuA4"
                     /note="Histone acetyltransferase subunit NuA4; pfam09340"
                     /db_xref="CDD:462763"
     Region          <109..>203
                     /region_name="ser_rich_anae_1"
                     /note="serine-rich protein; NF033849"
                     /db_xref="CDD:468206"
     CDS             1..215
                     /gene="Eaf6"
                     /coded_by="XM_034328905.2:568..1215"
                     /db_xref="GeneID:117606456"
ORIGIN      
        1 mstkssvdlr aelaelvkrk aeiadtlanl erqiyafegs yledtqlygn iirgwdryla
       61 snkntnskad krnrkfkeae rlfskssits maavsglven tqekidryse sesqignsgs
      121 edncslvnsh gttqnssssn gnkessgknh slsgqynqng sltssteisg qfanghasng
      181 slehvslken tgkepsknks gnsakknsnk rsrnr
//
LOCUS       XP_076545735             174 aa            linear   INV 03-SEP-2025
DEFINITION  chromatin modification-related protein EAF6/MEAF6 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545735
VERSION     XP_076545735.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689620.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..174
                     /product="chromatin modification-related protein
                     EAF6/MEAF6 isoform X2"
                     /calculated_mol_wt=19519
     Region          12..91
                     /region_name="NuA4"
                     /note="Histone acetyltransferase subunit NuA4; pfam09340"
                     /db_xref="CDD:462763"
     CDS             1..174
                     /gene="Eaf6"
                     /coded_by="XM_076689620.1:571..1095"
                     /db_xref="GeneID:117606456"
ORIGIN      
        1 mstkssvdlr aelaelvkrk aeiadtlanl erqiyafegs yledtqlygn iirgwdryla
       61 snkntnskad krnrkfkeae rlfskssits maltdtanrs hrlatavakt iaallilmeq
      121 privvaamvi rnqvektily lasiirtdls hlpprfldnl qmdmratvvs smsp
//
LOCUS       XP_034184798             211 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane emp24 domain-containing protein bai [Osmia lignaria
            lignaria].
ACCESSION   XP_034184798
VERSION     XP_034184798.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328907.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..211
                     /product="transmembrane emp24 domain-containing protein
                     bai"
                     /calculated_mol_wt=24462
     Region          17..205
                     /region_name="EMP24_GP25L"
                     /note="emp24/gp25L/p24 family/GOLD; pfam01105"
                     /db_xref="CDD:426051"
     CDS             1..211
                     /gene="bai"
                     /coded_by="XM_034328907.2:183..818"
                     /db_xref="GeneID:117606457"
ORIGIN      
        1 mknvifllvt liahvscirf ylepnsmkcl keevqanvlv ageyevsatp svkteytird
       61 skghilsrke diphgrllkf sfvteiydtf eicfksyaiq psfsgnikqi kqeihlitkr
      121 gieaknyegi geaaklkpse velkrledls yaivqdfarm rkneeemrdt neatntrvly
      181 fsifsmcwll vlstwqvlyl rrffkakkli e
//
LOCUS       XP_034184799             209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606458 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184799
VERSION     XP_034184799.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328908.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="uncharacterized protein LOC117606458 isoform X1"
                     /calculated_mol_wt=22467
     Region          125..177
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..209
                     /gene="LOC117606458"
                     /coded_by="XM_034328908.2:739..1368"
                     /db_xref="GeneID:117606458"
ORIGIN      
        1 mhwhtlediv qenllaacav vilccilklt iagvlqqrfp capvdaitgn gaavpvacal
       61 ksigskvepa lptyvkvsap iayrplpkts slvyiappif ktasavvate tksenqpefa
      121 aypkysfnyg vldgytgdsk saweerdgdt vkgeysvvea dgsirtvtyt addhngfnav
      181 vtrneppkns rqevnvkrfl pvtilpdsh
//
LOCUS       XP_076545736             209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606458 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545736
VERSION     XP_076545736.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689621.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="uncharacterized protein LOC117606458 isoform X1"
                     /calculated_mol_wt=22467
     Region          125..177
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..209
                     /gene="LOC117606458"
                     /coded_by="XM_076689621.1:116..745"
                     /db_xref="GeneID:117606458"
ORIGIN      
        1 mhwhtlediv qenllaacav vilccilklt iagvlqqrfp capvdaitgn gaavpvacal
       61 ksigskvepa lptyvkvsap iayrplpkts slvyiappif ktasavvate tksenqpefa
      121 aypkysfnyg vldgytgdsk saweerdgdt vkgeysvvea dgsirtvtyt addhngfnav
      181 vtrneppkns rqevnvkrfl pvtilpdsh
//
LOCUS       XP_034184800             202 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606458 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184800
VERSION     XP_034184800.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328909.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="uncharacterized protein LOC117606458 isoform X2"
                     /calculated_mol_wt=21697
     Region          118..170
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..202
                     /gene="LOC117606458"
                     /coded_by="XM_034328909.2:689..1297"
                     /db_xref="GeneID:117606458"
ORIGIN      
        1 mrlqfsksvs nkvvilccil kltiagvlqq rfpcapvdai tgngaavpva calksigskv
       61 epalptyvkv sapiayrplp ktsslvyiap pifktasavv atetksenqp efaaypkysf
      121 nygvldgytg dsksaweerd gdtvkgeysv veadgsirtv tytaddhngf navvtrnepp
      181 knsrqevnvk rflpvtilpd sh
//
LOCUS       XP_034184802             189 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 10 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184802
VERSION     XP_034184802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328911.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="anaphase-promoting complex subunit 10 isoform
                     X1"
                     /calculated_mol_wt=21408
     Region          6..187
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(53,80,87,89,152)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..189
                     /gene="APC10"
                     /coded_by="XM_034328911.2:117..686"
                     /db_xref="GeneID:117606461"
ORIGIN      
        1 mtnktgnage idpvqeelag rvhevgnhai wslssckpgf gvdqlrddit etywqsdgql
       61 phlvniqfkr kttirdiciy tdykldesyt psrisiragt nfndlqevev mdlnepsgwv
      121 iipikdindr pirtfmiqia visnhqngrd thmrqikvhs ptqdvlgppa syipgqfltn
      181 eflryatvr
//
LOCUS       XP_034184803             186 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 10 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184803
VERSION     XP_034184803.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328912.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..186
                     /product="anaphase-promoting complex subunit 10 isoform
                     X2"
                     /calculated_mol_wt=21166
     Region          3..184
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(50,77,84,86,149)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..186
                     /gene="APC10"
                     /coded_by="XM_034328912.2:116..676"
                     /db_xref="GeneID:117606461"
ORIGIN      
        1 mtnktgeidp vqeelagrvh evgnhaiwsl ssckpgfgvd qlrdditety wqsdgqlphl
       61 vniqfkrktt irdiciytdy kldesytpsr isiragtnfn dlqevevmdl nepsgwviip
      121 ikdindrpir tfmiqiavis nhqngrdthm rqikvhsptq dvlgppasyi pgqfltnefl
      181 ryatvr
//
LOCUS       XP_034184804             186 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 10 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184804
VERSION     XP_034184804.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328913.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..186
                     /product="anaphase-promoting complex subunit 10 isoform
                     X3"
                     /calculated_mol_wt=21109
     Region          3..184
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(50,77,84,86,149)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..186
                     /gene="APC10"
                     /coded_by="XM_034328913.2:114..674"
                     /db_xref="GeneID:117606461"
ORIGIN      
        1 mtnktgnadp vqeelagrvh evgnhaiwsl ssckpgfgvd qlrdditety wqsdgqlphl
       61 vniqfkrktt irdiciytdy kldesytpsr isiragtnfn dlqevevmdl nepsgwviip
      121 ikdindrpir tfmiqiavis nhqngrdthm rqikvhsptq dvlgppasyi pgqfltnefl
      181 ryatvr
//
LOCUS       XP_034184806             183 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 10 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184806
VERSION     XP_034184806.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328915.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..183
                     /product="anaphase-promoting complex subunit 10 isoform
                     X4"
                     /calculated_mol_wt=20867
     Region          2..181
                     /region_name="ANAPC10"
                     /note="Anaphase-promoting complex, subunit 10 (APC10);
                     pfam03256"
                     /db_xref="CDD:367420"
     Site            order(47,74,81,83,146)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     CDS             1..183
                     /gene="APC10"
                     /coded_by="XM_034328915.2:98..649"
                     /db_xref="GeneID:117606461"
ORIGIN      
        1 mtnktdpvqe elagrvhevg nhaiwslssc kpgfgvdqlr dditetywqs dgqlphlvni
       61 qfkrkttird iciytdykld esytpsrisi ragtnfndlq evevmdlnep sgwviipikd
      121 indrpirtfm iqiavisnhq ngrdthmrqi kvhsptqdvl gppasyipgq fltneflrya
      181 tvr
//
LOCUS       XP_034184807             180 aa            linear   INV 03-SEP-2025
DEFINITION  60S ribosome subunit biogenesis protein NIP7 homolog [Osmia
            lignaria lignaria].
ACCESSION   XP_034184807
VERSION     XP_034184807.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328916.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184807.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..180
                     /product="60S ribosome subunit biogenesis protein NIP7
                     homolog"
                     /calculated_mol_wt=20352
     Region          4..86
                     /region_name="Nip7_N_euk"
                     /note="N-terminal domain of eukaryotic 60S ribosome
                     subunit biogenesis protein Nip7 and similar proteins;
                     cd21146"
                     /db_xref="CDD:409283"
     Site            order(18..19,83..84,86)
                     /site_type="other"
                     /note="PUA domain interface [polypeptide binding]"
                     /db_xref="CDD:409283"
     Region          95..172
                     /region_name="PUA_Nip7-like"
                     /note="PUA RNA binding domain of ribosome assembly factor
                     Nip7 and similar proteins; cd21151"
                     /db_xref="CDD:409293"
     Site            order(100,106,108..110,112..114,163..166)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409293"
     CDS             1..180
                     /gene="LOC117606462"
                     /coded_by="XM_034328916.2:157..699"
                     /db_xref="GeneID:117606462"
ORIGIN      
        1 mkrlteeqtk lvlekltkyi ggnvkllidr pdgiycfrlr kdrvyyvsek ilplasmiss
       61 dhlisfgtcf gkftksgkfr lhitalyhla pyaqykiwlk psaeqqflyg hhvskgglsr
      121 itentpqyqg vvifstnnip lgfgvtakst mdckyadpta tvcfhqtdig eyirsedsli
//
LOCUS       XP_034184811             134 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 242 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184811
VERSION     XP_034184811.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328920.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..134
                     /product="transmembrane protein 242 isoform X1"
                     /calculated_mol_wt=14494
     Region          2..104
                     /region_name="DUF1358"
                     /note="Protein of unknown function (DUF1358); pfam07096"
                     /db_xref="CDD:429291"
     CDS             1..134
                     /gene="LOC117606464"
                     /coded_by="XM_034328920.2:195..599"
                     /db_xref="GeneID:117606464"
ORIGIN      
        1 mtsnedvkkk kdklygtlfl agiagistmv gfgtalastr kqdaknfeag lyesgaalat
       61 kalirgslwa iggcgllffg iwklsgakta eefrlkagsi lprvpknnpp qsrtefenlt
      121 dflryvseew gkek
//
LOCUS       XP_034184812             121 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 242 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184812
VERSION     XP_034184812.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328921.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..121
                     /product="transmembrane protein 242 isoform X2"
                     /calculated_mol_wt=12915
     Region          3..91
                     /region_name="DUF1358"
                     /note="Protein of unknown function (DUF1358); pfam07096"
                     /db_xref="CDD:429291"
     CDS             1..121
                     /gene="LOC117606464"
                     /coded_by="XM_034328921.2:253..618"
                     /db_xref="GeneID:117606464"
ORIGIN      
        1 mvgtlflagi agistmvgfg talastrkqd aknfeaglye sgaalatkal irgslwaigg
       61 cgllffgiwk lsgaktaeef rlkagsilpr vpknnppqsr tefenltdfl ryvseewgke
      121 k
//
LOCUS       XP_034184814             108 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerases I and III subunit AC2 l(2)37Cg [Osmia lignaria
            lignaria].
ACCESSION   XP_034184814
VERSION     XP_034184814.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328923.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..108
                     /product="RNA polymerases I and III subunit AC2 l(2)37Cg"
                     /calculated_mol_wt=11905
     Region          7..90
                     /region_name="RNAP_I_III_AC19"
                     /note="AC19 subunit of Eukaryotic RNA polymerase (RNAP) I
                     and RNAP III; cd07029"
                     /db_xref="CDD:132907"
     Site            order(21,26..28,30,35,49,76..77,82..84,86..88)
                     /site_type="other"
                     /note="AC19 - AC40 heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:132907"
     Site            order(26,51..52)
                     /site_type="other"
                     /note="AC19 - A135(C128) interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:132907"
     Site            order(33,44,46..48,50..52)
                     /site_type="other"
                     /note="AC19 - A190(C160) interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:132907"
     Site            43
                     /site_type="other"
                     /note="AC19 - RPB8 interaction site [polypeptide binding]"
                     /db_xref="CDD:132907"
     CDS             1..108
                     /gene="l(2)37Cg"
                     /coded_by="XM_034328923.2:123..449"
                     /db_xref="GeneID:117606466"
ORIGIN      
        1 mgrlaevase hsseqnktfv fmnegytlgn alvtvisqnp dvefcacfvn hpaegniylr
       61 iqakkgkavd vlrkglqdfe kicdytletf ngayeqfkss kdtsvdst
//
LOCUS       XP_034184815              91 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L37-1 [Osmia lignaria lignaria].
ACCESSION   XP_034184815
VERSION     XP_034184815.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328924.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..91
                     /product="ribosomal protein L37-1"
                     /calculated_mol_wt=10585
     Region          1..81
                     /region_name="PTZ00073"
                     /note="60S ribosomal protein L37; Provisional"
                     /db_xref="CDD:240257"
     CDS             1..91
                     /gene="RpL37-1"
                     /coded_by="XM_034328924.2:139..414"
                     /db_xref="GeneID:117606467"
ORIGIN      
        1 mtkgtssfgk rrnkthtlcr rcgrssyhiq ksqcaqcgyp qkkmrsynws ikakrrkttg
       61 tgrmrylkiv rrkfkngfre glpkpktvqs k
//
LOCUS       XP_076545737             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545737
VERSION     XP_076545737.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689622.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689622.1:2556..3935"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545738             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545738
VERSION     XP_076545738.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689623.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689623.1:2949..4328"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545739             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545739
VERSION     XP_076545739.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689624.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689624.1:2748..4127"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545740             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545740
VERSION     XP_076545740.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689625.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689625.1:2797..4176"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545741             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545741
VERSION     XP_076545741.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689626.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689626.1:2790..4169"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545742             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545742
VERSION     XP_076545742.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689627.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689627.1:2728..4107"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_034184756             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184756
VERSION     XP_034184756.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184756.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_034328865.2:2601..3980"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545743             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545743
VERSION     XP_076545743.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689628.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689628.1:2900..4279"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545744             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545744
VERSION     XP_076545744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689629.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689629.1:2794..4173"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545745             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545745
VERSION     XP_076545745.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689630.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689630.1:2671..4050"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545746             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545746
VERSION     XP_076545746.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689631.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689631.1:2884..4263"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545747             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545747
VERSION     XP_076545747.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689632.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689632.1:2570..3949"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545748             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545748
VERSION     XP_076545748.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689633.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689633.1:2629..4008"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_076545749             459 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545749
VERSION     XP_076545749.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689634.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X1"
                     /calculated_mol_wt=52862
     Region          36..458
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..459
                     /gene="TyrRS-m"
                     /coded_by="XM_076689634.1:2717..4096"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 msrilrlrlf tknksfnnrr lhtakyvlda enrqlyedif pdiyvneika lwrkppqcvy
       61 agfdptaesl hvgnllvlin llhwqrcghr viillggatg ligdpsyrks erveieeiil
      121 kenlnsiken ivnilrnhqk yfnkgrknll pvqilnniew ykdknvlsfi keigkyfrmg
      181 pmlgrasvqs rlqsengmsf teftyplfqg ydwlhlqqty ncnfqiggqd qmgnivsgyd
      241 lvsryikkhv ygmtlplvts eggkkfgkst gnavwlspsk sssfqlyqyf vrtedadvek
      301 flhfftflpi ryineiveeh fkkpelrtaq kilaekvtll vhgedglvaa krasavlyen
      361 sleslqrmna delvqlldga tvvqvfaepg invyelamka kcfktshdar riiegggfyi
      421 nyqritntee vivpgihils nnisllrvgk rtyhiirwl
//
LOCUS       XP_034184758             384 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine--tRNA ligase, mitochondrial isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184758
VERSION     XP_034184758.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328867.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184758.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="tyrosine--tRNA ligase, mitochondrial isoform X2"
                     /calculated_mol_wt=44118
     Region          22..383
                     /region_name="TyrS"
                     /note="Tyrosyl-tRNA synthetase [Translation, ribosomal
                     structure and biogenesis]; COG0162"
                     /db_xref="CDD:439932"
     CDS             1..384
                     /gene="TyrRS-m"
                     /coded_by="XM_034328867.2:81..1235"
                     /db_xref="GeneID:117606437"
ORIGIN      
        1 meettavclr wirsncrkft rgatgligdp syrkservei eeiilkenln sikenivnil
       61 rnhqkyfnkg rknllpvqil nniewykdkn vlsfikeigk yfrmgpmlgr asvqsrlqse
      121 ngmsftefty plfqgydwlh lqqtyncnfq iggqdqmgni vsgydlvsry ikkhvygmtl
      181 plvtseggkk fgkstgnavw lspsksssfq lyqyfvrted advekflhff tflpiryine
      241 iveehfkkpe lrtaqkilae kvtllvhged glvaakrasa vlyenslesl qrmnadelvq
      301 lldgatvvqv faepginvye lamkakcfkt shdarriieg ggfyinyqri tnteevivpg
      361 ihilsnnisl lrvgkrtyhi irwl
//
LOCUS       XP_034184767             350 aa            linear   INV 03-SEP-2025
DEFINITION  tweedleT [Osmia lignaria lignaria].
ACCESSION   XP_034184767
VERSION     XP_034184767.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328876.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="tweedleT"
                     /calculated_mol_wt=31394
     Region          156..252
                     /region_name="DUF243"
                     /note="Domain of unknown function (DUF243); pfam03103"
                     /db_xref="CDD:460806"
     CDS             1..350
                     /gene="TwdlT"
                     /coded_by="XM_034328876.2:1..1053"
                     /db_xref="GeneID:117606441"
ORIGIN      
        1 mgrdasgara gikgapwhlw sispliqgta atmrafmivv laasamarpe agysysqpss
       61 sygapggggs tgfgggvggg lgglgglggl gggggggfgg gggggggfgg gfgggvgggi
      121 gggfgggagg gigggfggga gggggggggf gggtliqkhi yvhvpppeap edrparpiaq
      181 ppppqkhyki ifikaptppt ptapvipalp qqdeqktliy vlvkkpeeap eislptiapt
      241 qpskpevyfi kyktqkevts gggggggggg ggggigggig ggigggvggg igggigggig
      301 ggigggiggg iggggigggi ggggldghgg sgpsgpstsy gapsasggpy
//
LOCUS       XP_076545750             404 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305562 [Osmia lignaria lignaria].
ACCESSION   XP_076545750
VERSION     XP_076545750.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689635.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="uncharacterized protein LOC143305562"
                     /calculated_mol_wt=46332
     Region          <309..339
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     CDS             1..404
                     /gene="LOC143305562"
                     /coded_by="XM_076689635.1:104..1318"
                     /db_xref="GeneID:143305562"
ORIGIN      
        1 mtasgpaaqe nvlrqlldrm qrmeeginqq lttlgeenqn rakeiqniae qlklvtlted
       61 astisritrr sggdqdnqqe demhtarqpp qnstirpnel mkavdairti etlrgrddvg
      121 vedfiksvry arsncsektl llklilieki tenakrsiry tsietyeely evlrqnvsip
      181 ntvsscrnkl qqirqgamet vqsynlrfrq qlneliyavq nkhqnpisrr iaieeeetea
      241 trtyvlnlrr digmlvipsk pkllieaqtm aadmelwtrd anramerrqv psripppkqq
      301 gqrpntssdr pnhsiplkct kcgrlghtsd kcyarnfqva nqgkippprv nnlnfqedkv
      361 tettsestap penyykfaae fqecresedy swtqeqests ssdq
//
LOCUS       XP_076545751             179 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611107 [Osmia lignaria lignaria].
ACCESSION   XP_076545751
VERSION     XP_076545751.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689636.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..179
                     /product="uncharacterized protein LOC117611107"
                     /calculated_mol_wt=19658
     CDS             1..179
                     /gene="LOC117611107"
                     /coded_by="XM_076689636.1:535..1074"
                     /db_xref="GeneID:117611107"
ORIGIN      
        1 micslhmrga kamqilplpg tagcipankh lvrqpsagwl aacirpgrfn qtivkradrv
       61 vlprcgwkkc sqsstgmalr vceklaggwv lagpqyttnc swkectrtnl ygykhcspei
      121 tsngwniqgg cwkksseirc lqvewprrfc tvaptvsgyd svglflldcs ipttrdheg
//
LOCUS       XP_034185312            5543 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606667 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185312
VERSION     XP_034185312.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329421.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185312.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..5543
                     /product="uncharacterized protein LOC117606667 isoform X1"
                     /calculated_mol_wt=627606
     Region          <121..335
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          449..470
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(454,456,458,460..461,464..465,469,495,497,501..502,
                     505..506,510,523,525,527,529..530,533..534)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          489..511
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          518..535
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <1402..2260
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <2379..2806
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <3772..>3978
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          <4641..5443
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <5170..5437
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..5543
                     /gene="LOC117606667"
                     /coded_by="XM_034329421.2:545..17176"
                     /db_xref="GeneID:117606667"
ORIGIN      
        1 mrertpsadd nprrappapl reaapadvtg sktssepvrr gpnfspatta sstsasspsl
       61 cvdarmvast hnldeltvsh ptsassdrqq qqekhgpayr saqtaqdkrs rlsnvinnlr
      121 kkvpdsrngd sprkeeddrn svernletle kyvmtvlngv ikdeeeedge vgrkkeekgk
      181 dpekmteeqe dedskgpaak fepskpnesr teaavfsskq pekpeakepl ghrksetres
      241 dvlqkeekvs svskeptdgf reenqalkve aaedkcssre tkpeddsvqe strtdaqeee
      301 rkenrslgti imerlsehqt eekgsieaak eqarefvpen velrnvcrdl lndllnginq
      361 lideksqate dsrpvegsve eskdsrspdl sttslhcslp ldkvasvlqn cqsnelvapq
      421 spssssssss sqgskspskp tsptvrhlcl ycdrkflsis lrqrhtervh qqgggrrser
      481 nsrrpsqncq ycsdkcaesl eslfqhmvgt hgdkyyacvq cstryltrea laghmsenha
      541 drnlqiqekt kepssspcke lgnqsprdrp dplspkerrs desdhehrae sillkpmism
      601 kdnfsnpgsp efdssfyssv scnirenllh hldgklqsss saiattsliv dsklqlqqqq
      661 tyyehsanqi qfpidislta atpvyskdya neeyennsey aqkpgkrnrs hprrvsfeky
      721 nfprkydgke qwtcsikdls kfdistqlsl rkkqqllker ytlsrlhqis lmspsgttdi
      781 aqdeqdlels speesksvee asggkkekli esmvegteam sprstefsse fenflrlrkw
      841 dektsneaak kqevvyaelt gewsrpriyi cgacatrhvt lkemedhkai shpnvwcshf
      901 efsgdqrely khlflpgkni ptakaraaml aekvctkcsk ncttlselhr hmlecggdqa
      961 wllglfgngk kkckwrpfgs rsrrrrqrgm krniqnsqtp rvntpkekqp tgprvrpsdr
     1021 esiqkmlanl ppkratrkvl qdstmrtqgr lrnvqtrtrp rmvgdnstas rmsrnkavlr
     1081 nkllknaksi qrnrcridni saviesvvrn yhpdktsddd kdavdgyidk etkeksidel
     1141 naelvneksn rtkvgrgprv lgkrrnvrtk dgatrgldqp rkgnlkarar gmlknpldpk
     1201 aegsseslka pkgvrvspkn vekeaevigp easpveaksp nflsknksst qttpkrkrpv
     1261 dpvaslnasi kaksqlrtqd gkfaknpnkd pfspvkssen kspvfgrqls kerineispe
     1321 tsvtrsklrt vsrnrnndqp pkrttrlssd sdkmptlepv vqiltnsedy teatndlpil
     1381 spvtpsssle kasrgknlig kvgarktmeg enteageavq veerdhkqrg rraksdlrnl
     1441 eknmtgkgeg gkvkeklkrk saparmlnve ekktvkniee tslkkelkqk keegtrtldt
     1501 rsnwktrtfd eepiklenvp fevkkligad nkddlmnklv ltsltstskr csrqsmtrvk
     1561 fgqtrckirk dtelekkads gsmsdastkk ekfearrksl rnterkfers rtvkeekfdq
     1621 ankiceavne vkasirgrrs sslsndskst mntgqvypsd kaeestvqns drrqtrgslm
     1681 nsseeqndvg evsqkkiast gkrrgrvrti ievsekknve adknsteptd kscpidqnea
     1741 klreesivkd kqkdedkepl kcpdvsetnk intnrlqvet sssvdsgken tletpapvqk
     1801 lkvgrprksw gakrertskr slnnvigilt egmnipaetq qsvplapqpa vdianpeeis
     1861 gssiqqasdd skpvnedkse kdsqkqiter agtpvqnpee escqpkaset seedtsvenc
     1921 kedssenskp skstkeelpt ddiildlsrr kqkgkgsfle kivskiakqk dallegevgs
     1981 lldtaadelt sildevgpsl seiaensvek ntrteaeqnv qgsdehstcr nlenkdtkae
     2041 geekaletsa tlkqskeeke nnegnidret svdkkpsddq lrpeekntes spdtskevin
     2101 skeiepqitp eeeckveifk edpnkdtkis lkpeesekls vsdqqssnek vskihkhpnk
     2161 eghrrprgrk pavknktvdl indseeseee dktelqnksk ivksvfgrvf gsektekvke
     2221 slndwvskse edsdlsranf vstssnvtna eatsearned knlkkhsvtp svpsttrkkp
     2281 grkpkdssss dkisqefsss yfvpvppnks rhckkiaeer ifrsfflgie qkktnyglrn
     2341 qnksdlsikd sdygsfsnde dfckmvsled tlqeeadpks rdaktedeam sseglskefc
     2401 neksshkela eesdskqeig psqlnpeyee naffkeqkes ticdaeigke fscpepspvl
     2461 neaqlltvqn iseekknqea vseetkikrk skkrslegns pkknkrksve pvdnseenli
     2521 pdeldladim elidkskqvp stkdnfeddl fveeriqcgk rktisedrvs knsdskmsqd
     2581 trvaeestei akklkvdsee kapdkkdete vernsiedse tardednssl llkisgkrks
     2641 yvasadhcqi pespvksssk rrsihdekss esskedalsr lesvegtesa gseitseiin
     2701 eeqiesssii nnnveecqss vnetlditer seireekpal enaenqqqsk edliknlpet
     2761 kptepvkspi tvkvlkkrgr kkknpvdiqt kvinqensql ppvnvdqvpr rslrtsryne
     2821 dpeernkisn daiksqtasp sleqfkvpev tevlqltkkr tykrrsttde hldgqnssps
     2881 vesemssaky setkslneke eqpsvhqkdf lenkqcsskg shqlglerss ssgsidlsqt
     2941 nrrrsskrkq lssedlsgqk nlnlraesvd nlsetsctse ssyfrkkrft krkkefqerr
     3001 edvqtdtsit dpesmdsekp adrrqslrrr akenisfeys afdlvdyfgi pmdmedspqe
     3061 dtvensvnqe pdstteelvq eptannisnq epeeistsed kstedtrkse veeeddskge
     3121 dkaddkleel pqsqddfqtp kkraagnfvv vhkktgeili vekrkkltke aarffcdvca
     3181 tsftrksslk khnqsqshlv qvtkfrkdkt lmeckenled tcsigqndvs sdenrsttdd
     3241 vkkdqkenfl dslydqtdlk svedesssyt gpadqeflmd shqriledel ldeeickite
     3301 nmshdeyvlt dhvspgpest stpikveikk teevgkkkkh erkkekakkk nlsneqvtld
     3361 ssetegpids smmnlldtsm smetsklsds dseysfllan slactlnkfr ekysqnagrt
     3421 eesqdgkdlt elkkdleqqe rtgysesnid gkaenlngkd vpkenhster lslkliinkr
     3481 slenfkeqdt pldrldtlak eneekpedpl dsfgepikei nqlepeidql nldmylgssk
     3541 qvenrksten iedfdtfkvq slgedladrq nsmvtenemq nesvketrhg kskkgkgrkh
     3601 qeeiessddt rrtskskdnq snqisegtng qkstdnsktl eyndenpell lydsqlestg
     3661 ikvnnvisps kveakneees delktiedqd srklqhelns lekvvlrhst eeqqlnqeti
     3721 dsknknssfn sldededsss ssladkplsq lllrtnlkvd tgkkllidsd delrpeekna
     3781 edspdtntst dnqecndkev snseqmepqv sseeecegei pkmetedtkt llepenevps
     3841 skdvddkesd dmdednnqks shgsnekvsk thkhsskegh rrskgkkapa rskiadltsd
     3901 segsededkt elqnkskivk svfgrvfgge kadkvkevln dwvsksedds dasrsdfrst
     3961 tpnelnaeet learneekrh lvtsvsstik kkpgrkpkds ssidrtkekn plsenreelr
     4021 gnrhsksrns sgnrkkpeee asdyyflvlp pnksrhskki aeerisrafd gvrmfeeflg
     4081 gdqkegnygl rsqskpdlti kdsgygsfny dedsskmvcs eepleeeqkl lkkrkslssk
     4141 tsksktdsdd wkdlvmemrs lksskeptde ednsvkdipe qeplssrlsa dlksrscssl
     4201 apssvrnrsp smdrnsqstr dtdvdeeded igrgrisplf vretpgssie sssnseneed
     4261 denvlgnest vrkrsssefs gekivirsps sthkgevvti aptdaiedna ldvpqeieta
     4321 kprqgkvlnf deelfveccs rlkattenel rgakkikldh negyhrkdeq qqglrgprdr
     4381 wrdvesqnsl gsllesvnqf nefqllgeem ystrerdypk ragrnlrseh ssrsaspdms
     4441 radnlgyeds ldvafehnnk lrdkiqqrmr esenliastf gkssndiahh nknneqdlrn
     4501 syshlqdqgq vsgaslsnrs ysdavhirnl qeqqnaakmh ldsgfknkmn sslgglldka
     4561 lsnllhsngk hdhngstpmk vlaelacara ptsttglpis qelvqpakts pnkesipdpc
     4621 pvtvpkdiqk kprnpikelf erkkemnerk qqekskteaa lrelnvhrqr ktkkmkkhqd
     4681 fplirrgehg gglgerkkrr dgferkeefv sdrikdvydf deeesqiepn lgsvmsyrsr
     4741 pgyevsclrt kevdvaglms kaigesleng kagdalssrl esmidrkfke lekfapktkg
     4801 alksfqsedq rqitgpmdef verkqlkskr pveqslkhsk lkkrnknpkk ktrnawyend
     4861 ssdeyrttav kpedigvgis ksqrtcskgk qnifaelyts sesefehdgv nyeskkqrra
     4921 kkgakkvaes edlkqnqesv nkyssenedr enddwrdqdl kddvkhesdh kksesemsdh
     4981 plviderkdg edqrnsdeet etqyernyem ddlyredssi vdsdvedsvt teaqnvpdes
     5041 rvknsnslqe kingktncir delipleeal dmldqadnea seanynvdrn vnsledgdpv
     5101 itaepdiset fnpveesavk spslleeqdq eleqeeepdn dllslpekls snekpqkesd
     5161 nlplhvflsr kvqeskkrke qqlkkmqeeq erilmdfqpt rrqrkcaigk qgllaeisss
     5221 deeisprdrr sndksdhdkp rkqkreskek rkeryiekkh eqmiakeqka ieeeilrevg
     5281 kkkdslamnp qncksdlsda hdpeklesme dkidqevmqk kkhqakekqk kqskvdeein
     5341 vkhnnegldd pegncnksil aesnhedtnn qespvkqkrh sksptkpkrt skgdakvask
     5401 rnrnansdks rnradskgsr dkerrssngk hdsddeelkt tkswnkveeg vgvaigrrkr
     5461 aaanqlyyws sssdeeemle vvpvveeeed drqeqhgwiv gdshkrmitm lamekqlkek
     5521 rrrsedefep grakskkhrn sts
//
LOCUS       XP_034185313            5543 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606667 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185313
VERSION     XP_034185313.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329422.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185313.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..5543
                     /product="uncharacterized protein LOC117606667 isoform X1"
                     /calculated_mol_wt=627606
     Region          <121..335
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          449..470
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(454,456,458,460..461,464..465,469,495,497,501..502,
                     505..506,510,523,525,527,529..530,533..534)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          489..511
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          518..535
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <1402..2260
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <2379..2806
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <3772..>3978
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          <4641..5443
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <5170..5437
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..5543
                     /gene="LOC117606667"
                     /coded_by="XM_034329422.2:545..17176"
                     /db_xref="GeneID:117606667"
ORIGIN      
        1 mrertpsadd nprrappapl reaapadvtg sktssepvrr gpnfspatta sstsasspsl
       61 cvdarmvast hnldeltvsh ptsassdrqq qqekhgpayr saqtaqdkrs rlsnvinnlr
      121 kkvpdsrngd sprkeeddrn svernletle kyvmtvlngv ikdeeeedge vgrkkeekgk
      181 dpekmteeqe dedskgpaak fepskpnesr teaavfsskq pekpeakepl ghrksetres
      241 dvlqkeekvs svskeptdgf reenqalkve aaedkcssre tkpeddsvqe strtdaqeee
      301 rkenrslgti imerlsehqt eekgsieaak eqarefvpen velrnvcrdl lndllnginq
      361 lideksqate dsrpvegsve eskdsrspdl sttslhcslp ldkvasvlqn cqsnelvapq
      421 spssssssss sqgskspskp tsptvrhlcl ycdrkflsis lrqrhtervh qqgggrrser
      481 nsrrpsqncq ycsdkcaesl eslfqhmvgt hgdkyyacvq cstryltrea laghmsenha
      541 drnlqiqekt kepssspcke lgnqsprdrp dplspkerrs desdhehrae sillkpmism
      601 kdnfsnpgsp efdssfyssv scnirenllh hldgklqsss saiattsliv dsklqlqqqq
      661 tyyehsanqi qfpidislta atpvyskdya neeyennsey aqkpgkrnrs hprrvsfeky
      721 nfprkydgke qwtcsikdls kfdistqlsl rkkqqllker ytlsrlhqis lmspsgttdi
      781 aqdeqdlels speesksvee asggkkekli esmvegteam sprstefsse fenflrlrkw
      841 dektsneaak kqevvyaelt gewsrpriyi cgacatrhvt lkemedhkai shpnvwcshf
      901 efsgdqrely khlflpgkni ptakaraaml aekvctkcsk ncttlselhr hmlecggdqa
      961 wllglfgngk kkckwrpfgs rsrrrrqrgm krniqnsqtp rvntpkekqp tgprvrpsdr
     1021 esiqkmlanl ppkratrkvl qdstmrtqgr lrnvqtrtrp rmvgdnstas rmsrnkavlr
     1081 nkllknaksi qrnrcridni saviesvvrn yhpdktsddd kdavdgyidk etkeksidel
     1141 naelvneksn rtkvgrgprv lgkrrnvrtk dgatrgldqp rkgnlkarar gmlknpldpk
     1201 aegsseslka pkgvrvspkn vekeaevigp easpveaksp nflsknksst qttpkrkrpv
     1261 dpvaslnasi kaksqlrtqd gkfaknpnkd pfspvkssen kspvfgrqls kerineispe
     1321 tsvtrsklrt vsrnrnndqp pkrttrlssd sdkmptlepv vqiltnsedy teatndlpil
     1381 spvtpsssle kasrgknlig kvgarktmeg enteageavq veerdhkqrg rraksdlrnl
     1441 eknmtgkgeg gkvkeklkrk saparmlnve ekktvkniee tslkkelkqk keegtrtldt
     1501 rsnwktrtfd eepiklenvp fevkkligad nkddlmnklv ltsltstskr csrqsmtrvk
     1561 fgqtrckirk dtelekkads gsmsdastkk ekfearrksl rnterkfers rtvkeekfdq
     1621 ankiceavne vkasirgrrs sslsndskst mntgqvypsd kaeestvqns drrqtrgslm
     1681 nsseeqndvg evsqkkiast gkrrgrvrti ievsekknve adknsteptd kscpidqnea
     1741 klreesivkd kqkdedkepl kcpdvsetnk intnrlqvet sssvdsgken tletpapvqk
     1801 lkvgrprksw gakrertskr slnnvigilt egmnipaetq qsvplapqpa vdianpeeis
     1861 gssiqqasdd skpvnedkse kdsqkqiter agtpvqnpee escqpkaset seedtsvenc
     1921 kedssenskp skstkeelpt ddiildlsrr kqkgkgsfle kivskiakqk dallegevgs
     1981 lldtaadelt sildevgpsl seiaensvek ntrteaeqnv qgsdehstcr nlenkdtkae
     2041 geekaletsa tlkqskeeke nnegnidret svdkkpsddq lrpeekntes spdtskevin
     2101 skeiepqitp eeeckveifk edpnkdtkis lkpeesekls vsdqqssnek vskihkhpnk
     2161 eghrrprgrk pavknktvdl indseeseee dktelqnksk ivksvfgrvf gsektekvke
     2221 slndwvskse edsdlsranf vstssnvtna eatsearned knlkkhsvtp svpsttrkkp
     2281 grkpkdssss dkisqefsss yfvpvppnks rhckkiaeer ifrsfflgie qkktnyglrn
     2341 qnksdlsikd sdygsfsnde dfckmvsled tlqeeadpks rdaktedeam sseglskefc
     2401 neksshkela eesdskqeig psqlnpeyee naffkeqkes ticdaeigke fscpepspvl
     2461 neaqlltvqn iseekknqea vseetkikrk skkrslegns pkknkrksve pvdnseenli
     2521 pdeldladim elidkskqvp stkdnfeddl fveeriqcgk rktisedrvs knsdskmsqd
     2581 trvaeestei akklkvdsee kapdkkdete vernsiedse tardednssl llkisgkrks
     2641 yvasadhcqi pespvksssk rrsihdekss esskedalsr lesvegtesa gseitseiin
     2701 eeqiesssii nnnveecqss vnetlditer seireekpal enaenqqqsk edliknlpet
     2761 kptepvkspi tvkvlkkrgr kkknpvdiqt kvinqensql ppvnvdqvpr rslrtsryne
     2821 dpeernkisn daiksqtasp sleqfkvpev tevlqltkkr tykrrsttde hldgqnssps
     2881 vesemssaky setkslneke eqpsvhqkdf lenkqcsskg shqlglerss ssgsidlsqt
     2941 nrrrsskrkq lssedlsgqk nlnlraesvd nlsetsctse ssyfrkkrft krkkefqerr
     3001 edvqtdtsit dpesmdsekp adrrqslrrr akenisfeys afdlvdyfgi pmdmedspqe
     3061 dtvensvnqe pdstteelvq eptannisnq epeeistsed kstedtrkse veeeddskge
     3121 dkaddkleel pqsqddfqtp kkraagnfvv vhkktgeili vekrkkltke aarffcdvca
     3181 tsftrksslk khnqsqshlv qvtkfrkdkt lmeckenled tcsigqndvs sdenrsttdd
     3241 vkkdqkenfl dslydqtdlk svedesssyt gpadqeflmd shqriledel ldeeickite
     3301 nmshdeyvlt dhvspgpest stpikveikk teevgkkkkh erkkekakkk nlsneqvtld
     3361 ssetegpids smmnlldtsm smetsklsds dseysfllan slactlnkfr ekysqnagrt
     3421 eesqdgkdlt elkkdleqqe rtgysesnid gkaenlngkd vpkenhster lslkliinkr
     3481 slenfkeqdt pldrldtlak eneekpedpl dsfgepikei nqlepeidql nldmylgssk
     3541 qvenrksten iedfdtfkvq slgedladrq nsmvtenemq nesvketrhg kskkgkgrkh
     3601 qeeiessddt rrtskskdnq snqisegtng qkstdnsktl eyndenpell lydsqlestg
     3661 ikvnnvisps kveakneees delktiedqd srklqhelns lekvvlrhst eeqqlnqeti
     3721 dsknknssfn sldededsss ssladkplsq lllrtnlkvd tgkkllidsd delrpeekna
     3781 edspdtntst dnqecndkev snseqmepqv sseeecegei pkmetedtkt llepenevps
     3841 skdvddkesd dmdednnqks shgsnekvsk thkhsskegh rrskgkkapa rskiadltsd
     3901 segsededkt elqnkskivk svfgrvfgge kadkvkevln dwvsksedds dasrsdfrst
     3961 tpnelnaeet learneekrh lvtsvsstik kkpgrkpkds ssidrtkekn plsenreelr
     4021 gnrhsksrns sgnrkkpeee asdyyflvlp pnksrhskki aeerisrafd gvrmfeeflg
     4081 gdqkegnygl rsqskpdlti kdsgygsfny dedsskmvcs eepleeeqkl lkkrkslssk
     4141 tsksktdsdd wkdlvmemrs lksskeptde ednsvkdipe qeplssrlsa dlksrscssl
     4201 apssvrnrsp smdrnsqstr dtdvdeeded igrgrisplf vretpgssie sssnseneed
     4261 denvlgnest vrkrsssefs gekivirsps sthkgevvti aptdaiedna ldvpqeieta
     4321 kprqgkvlnf deelfveccs rlkattenel rgakkikldh negyhrkdeq qqglrgprdr
     4381 wrdvesqnsl gsllesvnqf nefqllgeem ystrerdypk ragrnlrseh ssrsaspdms
     4441 radnlgyeds ldvafehnnk lrdkiqqrmr esenliastf gkssndiahh nknneqdlrn
     4501 syshlqdqgq vsgaslsnrs ysdavhirnl qeqqnaakmh ldsgfknkmn sslgglldka
     4561 lsnllhsngk hdhngstpmk vlaelacara ptsttglpis qelvqpakts pnkesipdpc
     4621 pvtvpkdiqk kprnpikelf erkkemnerk qqekskteaa lrelnvhrqr ktkkmkkhqd
     4681 fplirrgehg gglgerkkrr dgferkeefv sdrikdvydf deeesqiepn lgsvmsyrsr
     4741 pgyevsclrt kevdvaglms kaigesleng kagdalssrl esmidrkfke lekfapktkg
     4801 alksfqsedq rqitgpmdef verkqlkskr pveqslkhsk lkkrnknpkk ktrnawyend
     4861 ssdeyrttav kpedigvgis ksqrtcskgk qnifaelyts sesefehdgv nyeskkqrra
     4921 kkgakkvaes edlkqnqesv nkyssenedr enddwrdqdl kddvkhesdh kksesemsdh
     4981 plviderkdg edqrnsdeet etqyernyem ddlyredssi vdsdvedsvt teaqnvpdes
     5041 rvknsnslqe kingktncir delipleeal dmldqadnea seanynvdrn vnsledgdpv
     5101 itaepdiset fnpveesavk spslleeqdq eleqeeepdn dllslpekls snekpqkesd
     5161 nlplhvflsr kvqeskkrke qqlkkmqeeq erilmdfqpt rrqrkcaigk qgllaeisss
     5221 deeisprdrr sndksdhdkp rkqkreskek rkeryiekkh eqmiakeqka ieeeilrevg
     5281 kkkdslamnp qncksdlsda hdpeklesme dkidqevmqk kkhqakekqk kqskvdeein
     5341 vkhnnegldd pegncnksil aesnhedtnn qespvkqkrh sksptkpkrt skgdakvask
     5401 rnrnansdks rnradskgsr dkerrssngk hdsddeelkt tkswnkveeg vgvaigrrkr
     5461 aaanqlyyws sssdeeemle vvpvveeeed drqeqhgwiv gdshkrmitm lamekqlkek
     5521 rrrsedefep grakskkhrn sts
//
LOCUS       XP_034185314            5538 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606667 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185314
VERSION     XP_034185314.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329423.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185314.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..5538
                     /product="uncharacterized protein LOC117606667 isoform X2"
                     /calculated_mol_wt=626940
     Region          <121..335
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          449..470
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(454,456,458,460..461,464..465,469,495,497,501..502,
                     505..506,510,523,525,527,529..530,533..534)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          489..511
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          518..535
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <1402..2260
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <2379..2806
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <3772..>3978
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          <4636..5438
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <5165..5432
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..5538
                     /gene="LOC117606667"
                     /coded_by="XM_034329423.2:545..17161"
                     /db_xref="GeneID:117606667"
ORIGIN      
        1 mrertpsadd nprrappapl reaapadvtg sktssepvrr gpnfspatta sstsasspsl
       61 cvdarmvast hnldeltvsh ptsassdrqq qqekhgpayr saqtaqdkrs rlsnvinnlr
      121 kkvpdsrngd sprkeeddrn svernletle kyvmtvlngv ikdeeeedge vgrkkeekgk
      181 dpekmteeqe dedskgpaak fepskpnesr teaavfsskq pekpeakepl ghrksetres
      241 dvlqkeekvs svskeptdgf reenqalkve aaedkcssre tkpeddsvqe strtdaqeee
      301 rkenrslgti imerlsehqt eekgsieaak eqarefvpen velrnvcrdl lndllnginq
      361 lideksqate dsrpvegsve eskdsrspdl sttslhcslp ldkvasvlqn cqsnelvapq
      421 spssssssss sqgskspskp tsptvrhlcl ycdrkflsis lrqrhtervh qqgggrrser
      481 nsrrpsqncq ycsdkcaesl eslfqhmvgt hgdkyyacvq cstryltrea laghmsenha
      541 drnlqiqekt kepssspcke lgnqsprdrp dplspkerrs desdhehrae sillkpmism
      601 kdnfsnpgsp efdssfyssv scnirenllh hldgklqsss saiattsliv dsklqlqqqq
      661 tyyehsanqi qfpidislta atpvyskdya neeyennsey aqkpgkrnrs hprrvsfeky
      721 nfprkydgke qwtcsikdls kfdistqlsl rkkqqllker ytlsrlhqis lmspsgttdi
      781 aqdeqdlels speesksvee asggkkekli esmvegteam sprstefsse fenflrlrkw
      841 dektsneaak kqevvyaelt gewsrpriyi cgacatrhvt lkemedhkai shpnvwcshf
      901 efsgdqrely khlflpgkni ptakaraaml aekvctkcsk ncttlselhr hmlecggdqa
      961 wllglfgngk kkckwrpfgs rsrrrrqrgm krniqnsqtp rvntpkekqp tgprvrpsdr
     1021 esiqkmlanl ppkratrkvl qdstmrtqgr lrnvqtrtrp rmvgdnstas rmsrnkavlr
     1081 nkllknaksi qrnrcridni saviesvvrn yhpdktsddd kdavdgyidk etkeksidel
     1141 naelvneksn rtkvgrgprv lgkrrnvrtk dgatrgldqp rkgnlkarar gmlknpldpk
     1201 aegsseslka pkgvrvspkn vekeaevigp easpveaksp nflsknksst qttpkrkrpv
     1261 dpvaslnasi kaksqlrtqd gkfaknpnkd pfspvkssen kspvfgrqls kerineispe
     1321 tsvtrsklrt vsrnrnndqp pkrttrlssd sdkmptlepv vqiltnsedy teatndlpil
     1381 spvtpsssle kasrgknlig kvgarktmeg enteageavq veerdhkqrg rraksdlrnl
     1441 eknmtgkgeg gkvkeklkrk saparmlnve ekktvkniee tslkkelkqk keegtrtldt
     1501 rsnwktrtfd eepiklenvp fevkkligad nkddlmnklv ltsltstskr csrqsmtrvk
     1561 fgqtrckirk dtelekkads gsmsdastkk ekfearrksl rnterkfers rtvkeekfdq
     1621 ankiceavne vkasirgrrs sslsndskst mntgqvypsd kaeestvqns drrqtrgslm
     1681 nsseeqndvg evsqkkiast gkrrgrvrti ievsekknve adknsteptd kscpidqnea
     1741 klreesivkd kqkdedkepl kcpdvsetnk intnrlqvet sssvdsgken tletpapvqk
     1801 lkvgrprksw gakrertskr slnnvigilt egmnipaetq qsvplapqpa vdianpeeis
     1861 gssiqqasdd skpvnedkse kdsqkqiter agtpvqnpee escqpkaset seedtsvenc
     1921 kedssenskp skstkeelpt ddiildlsrr kqkgkgsfle kivskiakqk dallegevgs
     1981 lldtaadelt sildevgpsl seiaensvek ntrteaeqnv qgsdehstcr nlenkdtkae
     2041 geekaletsa tlkqskeeke nnegnidret svdkkpsddq lrpeekntes spdtskevin
     2101 skeiepqitp eeeckveifk edpnkdtkis lkpeesekls vsdqqssnek vskihkhpnk
     2161 eghrrprgrk pavknktvdl indseeseee dktelqnksk ivksvfgrvf gsektekvke
     2221 slndwvskse edsdlsranf vstssnvtna eatsearned knlkkhsvtp svpsttrkkp
     2281 grkpkdssss dkisqefsss yfvpvppnks rhckkiaeer ifrsfflgie qkktnyglrn
     2341 qnksdlsikd sdygsfsnde dfckmvsled tlqeeadpks rdaktedeam sseglskefc
     2401 neksshkela eesdskqeig psqlnpeyee naffkeqkes ticdaeigke fscpepspvl
     2461 neaqlltvqn iseekknqea vseetkikrk skkrslegns pkknkrksve pvdnseenli
     2521 pdeldladim elidkskqvp stkdnfeddl fveeriqcgk rktisedrvs knsdskmsqd
     2581 trvaeestei akklkvdsee kapdkkdete vernsiedse tardednssl llkisgkrks
     2641 yvasadhcqi pespvksssk rrsihdekss esskedalsr lesvegtesa gseitseiin
     2701 eeqiesssii nnnveecqss vnetlditer seireekpal enaenqqqsk edliknlpet
     2761 kptepvkspi tvkvlkkrgr kkknpvdiqt kvinqensql ppvnvdqvpr rslrtsryne
     2821 dpeernkisn daiksqtasp sleqfkvpev tevlqltkkr tykrrsttde hldgqnssps
     2881 vesemssaky setkslneke eqpsvhqkdf lenkqcsskg shqlglerss ssgsidlsqt
     2941 nrrrsskrkq lssedlsgqk nlnlraesvd nlsetsctse ssyfrkkrft krkkefqerr
     3001 edvqtdtsit dpesmdsekp adrrqslrrr akenisfeys afdlvdyfgi pmdmedspqe
     3061 dtvensvnqe pdstteelvq eptannisnq epeeistsed kstedtrkse veeeddskge
     3121 dkaddkleel pqsqddfqtp kkraagnfvv vhkktgeili vekrkkltke aarffcdvca
     3181 tsftrksslk khnqsqshlv qvtkfrkdkt lmeckenled tcsigqndvs sdenrsttdd
     3241 vkkdqkenfl dslydqtdlk svedesssyt gpadqeflmd shqriledel ldeeickite
     3301 nmshdeyvlt dhvspgpest stpikveikk teevgkkkkh erkkekakkk nlsneqvtld
     3361 ssetegpids smmnlldtsm smetsklsds dseysfllan slactlnkfr ekysqnagrt
     3421 eesqdgkdlt elkkdleqqe rtgysesnid gkaenlngkd vpkenhster lslkliinkr
     3481 slenfkeqdt pldrldtlak eneekpedpl dsfgepikei nqlepeidql nldmylgssk
     3541 qvenrksten iedfdtfkvq slgedladrq nsmvtenemq nesvketrhg kskkgkgrkh
     3601 qeeiessddt rrtskskdnq snqisegtng qkstdnsktl eyndenpell lydsqlestg
     3661 ikvnnvisps kveakneees delktiedqd srklqhelns lekvvlrhst eeqqlnqeti
     3721 dsknknssfn sldededsss ssladkplsq lllrtnlkvd tgkkllidsd delrpeekna
     3781 edspdtntst dnqecndkev snseqmepqv sseeecegei pkmetedtkt llepenevps
     3841 skdvddkesd dmdednnqks shgsnekvsk thkhsskegh rrskgkkapa rskiadltsd
     3901 segsededkt elqnkskivk svfgrvfgge kadkvkevln dwvsksedds dasrsdfrst
     3961 tpnelnaeet learneekrh lvtsvsstik kkpgrkpkds ssidrtkekn plsenreelr
     4021 gnrhsksrns sgnrkkpeee asdyyflvlp pnksrhskki aeerisrafd gvrmfeeflg
     4081 gdqkegnygl rsqskpdlti kdsgygsfny dedsskmvcs eepleeeqkl lkkrkslssk
     4141 tsksktdsdd wkdlvmemrs lksskeptde ednsvkdipe qeplssrlsa dlksrscssl
     4201 apssvrnrsp smdrnsqstr dtdvdeeded igrgrisplf vretpgssie sssnseneed
     4261 denvlgnest vrkrsssefs gekivirsps sthkgevvti aptdaiedna ldvpqeieta
     4321 kprqgkvlnf deelfveccs rlkattenel rgakkikldh negyhrkdeq qqglrgprdr
     4381 wrdvesqnsl gsllesvnql lgeemystre rdypkragrn lrsehssrsa spdmsradnl
     4441 gyedsldvaf ehnnklrdki qqrmresenl iastfgkssn diahhnknne qdlrnsyshl
     4501 qdqgqvsgas lsnrsysdav hirnlqeqqn aakmhldsgf knkmnsslgg lldkalsnll
     4561 hsngkhdhng stpmkvlael acaraptstt glpisqelvq paktspnkes ipdpcpvtvp
     4621 kdiqkkprnp ikelferkke mnerkqqeks kteaalreln vhrqrktkkm kkhqdfplir
     4681 rgehggglge rkkrrdgfer keefvsdrik dvydfdeees qiepnlgsvm syrsrpgyev
     4741 sclrtkevdv aglmskaige slengkagda lssrlesmid rkfkelekfa pktkgalksf
     4801 qsedqrqitg pmdefverkq lkskrpveqs lkhsklkkrn knpkkktrna wyendssdey
     4861 rttavkpedi gvgisksqrt cskgkqnifa elytssesef ehdgvnyesk kqrrakkgak
     4921 kvaesedlkq nqesvnkyss enedrenddw rdqdlkddvk hesdhkkses emsdhplvid
     4981 erkdgedqrn sdeetetqye rnyemddlyr edssivdsdv edsvtteaqn vpdesrvkns
     5041 nslqekingk tncirdelip leealdmldq adneaseany nvdrnvnsle dgdpvitaep
     5101 disetfnpve esavkspsll eeqdqeleqe eepdndllsl peklssnekp qkesdnlplh
     5161 vflsrkvqes kkrkeqqlkk mqeeqerilm dfqptrrqrk caigkqglla eisssdeeis
     5221 prdrrsndks dhdkprkqkr eskekrkery iekkheqmia keqkaieeei lrevgkkkds
     5281 lamnpqncks dlsdahdpek lesmedkidq evmqkkkhqa kekqkkqskv deeinvkhnn
     5341 eglddpegnc nksilaesnh edtnnqespv kqkrhskspt kpkrtskgda kvaskrnrna
     5401 nsdksrnrad skgsrdkerr ssngkhdsdd eelkttkswn kveegvgvai grrkraaanq
     5461 lyywssssde eemlevvpvv eeeeddrqeq hgwivgdshk rmitmlamek qlkekrrrse
     5521 defepgraks kkhrnsts
//
LOCUS       XP_076545752            4660 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606667 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545752
VERSION     XP_076545752.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689637.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4660
                     /product="uncharacterized protein LOC117606667 isoform X3"
                     /calculated_mol_wt=528280
     Region          <519..1377
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <1496..1923
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <2889..>3095
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          <4287..4554
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..4660
                     /gene="LOC117606667"
                     /coded_by="XM_076689637.1:128..14110"
                     /db_xref="GeneID:117606667"
ORIGIN      
        1 medhkaishp nvwcshfefs gdqrelykhl flpgknipta karaamlaek vctkcsknct
       61 tlselhrhml ecggdqawll glfgngkkkc kwrpfgsrsr rrrqrgmkrn iqnsqtprvn
      121 tpkekqptgp rvrpsdresi qkmlanlppk ratrkvlqds tmrtqgrlrn vqtrtrprmv
      181 gdnstasrms rnkavlrnkl lknaksiqrn rcridnisav iesvvrnyhp dktsdddkda
      241 vdgyidketk eksidelnae lvneksnrtk vgrgprvlgk rrnvrtkdga trgldqprkg
      301 nlkarargml knpldpkaeg sseslkapkg vrvspknvek eaevigpeas pveakspnfl
      361 sknksstqtt pkrkrpvdpv aslnasikak sqlrtqdgkf aknpnkdpfs pvkssenksp
      421 vfgrqlsker ineispetsv trsklrtvsr nrnndqppkr ttrlssdsdk mptlepvvqi
      481 ltnsedytea tndlpilspv tpssslekas rgknligkvg arktmegent eageavqvee
      541 rdhkqrgrra ksdlrnlekn mtgkgeggkv keklkrksap armlnveekk tvknieetsl
      601 kkelkqkkee gtrtldtrsn wktrtfdeep iklenvpfev kkligadnkd dlmnklvlts
      661 ltstskrcsr qsmtrvkfgq trckirkdte lekkadsgsm sdastkkekf earrkslrnt
      721 erkfersrtv keekfdqank iceavnevka sirgrrsssl sndskstmnt gqvypsdkae
      781 estvqnsdrr qtrgslmnss eeqndvgevs qkkiastgkr rgrvrtiiev sekknveadk
      841 nsteptdksc pidqneaklr eesivkdkqk dedkeplkcp dvsetnkint nrlqvetsss
      901 vdsgkentle tpapvqklkv grprkswgak rertskrsln nvigiltegm nipaetqqsv
      961 plapqpavdi anpeeisgss iqqasddskp vnedksekds qkqiteragt pvqnpeeesc
     1021 qpkasetsee dtsvencked ssenskpsks tkeelptddi ildlsrrkqk gkgsflekiv
     1081 skiakqkdal legevgslld taadeltsil devgpslsei aensvekntr teaeqnvqgs
     1141 dehstcrnle nkdtkaegee kaletsatlk qskeekenne gnidretsvd kkpsddqlrp
     1201 eekntesspd tskevinske iepqitpeee ckveifkedp nkdtkislkp eeseklsvsd
     1261 qqssnekvsk ihkhpnkegh rrprgrkpav knktvdlind seeseeedkt elqnkskivk
     1321 svfgrvfgse ktekvkesln dwvskseeds dlsranfvst ssnvtnaeat searnedknl
     1381 kkhsvtpsvp sttrkkpgrk pkdssssdki sqefsssyfv pvppnksrhc kkiaeerifr
     1441 sfflgieqkk tnyglrnqnk sdlsikdsdy gsfsndedfc kmvsledtlq eeadpksrda
     1501 ktedeamsse glskefcnek sshkelaees dskqeigpsq lnpeyeenaf fkeqkestic
     1561 daeigkefsc pepspvlnea qlltvqnise ekknqeavse etkikrkskk rslegnspkk
     1621 nkrksvepvd nseenlipde ldladimeli dkskqvpstk dnfeddlfve eriqcgkrkt
     1681 isedrvskns dskmsqdtrv aeesteiakk lkvdseekap dkkdetever nsiedsetar
     1741 dednsslllk isgkrksyva sadhcqipes pvkssskrrs ihdekssess kedalsrles
     1801 vegtesagse itseiineeq iesssiinnn veecqssvne tlditersei reekpalena
     1861 enqqqskedl iknlpetkpt epvkspitvk vlkkrgrkkk npvdiqtkvi nqensqlppv
     1921 nvdqvprrsl rtsrynedpe ernkisndai ksqtaspsle qfkvpevtev lqltkkrtyk
     1981 rrsttdehld gqnsspsves emssakyset kslnekeeqp svhqkdflen kqcsskgshq
     2041 lglerssssg sidlsqtnrr rsskrkqlss edlsgqknln lraesvdnls etsctsessy
     2101 frkkrftkrk kefqerredv qtdtsitdpe smdsekpadr rqslrrrake nisfeysafd
     2161 lvdyfgipmd medspqedtv ensvnqepds tteelvqept annisnqepe eistsedkst
     2221 edtrksevee eddskgedka ddkleelpqs qddfqtpkkr aagnfvvvhk ktgeilivek
     2281 rkkltkeaar ffcdvcatsf trksslkkhn qsqshlvqvt kfrkdktlme ckenledtcs
     2341 igqndvssde nrsttddvkk dqkenfldsl ydqtdlksve desssytgpa dqeflmdshq
     2401 riledellde eickitenms hdeyvltdhv spgpeststp ikveikktee vgkkkkherk
     2461 kekakkknls neqvtldsse tegpidssmm nlldtsmsme tsklsdsdse ysfllansla
     2521 ctlnkfreky sqnagrtees qdgkdltelk kdleqqertg ysesnidgka enlngkdvpk
     2581 enhsterlsl kliinkrsle nfkeqdtpld rldtlakene ekpedpldsf gepikeinql
     2641 epeidqlnld mylgsskqve nrkstenied fdtfkvqslg edladrqnsm vtenemqnes
     2701 vketrhgksk kgkgrkhqee iessddtrrt skskdnqsnq isegtngqks tdnsktleyn
     2761 denpelllyd sqlestgikv nnvispskve akneeesdel ktiedqdsrk lqhelnslek
     2821 vvlrhsteeq qlnqetidsk nknssfnsld ededsssssl adkplsqlll rtnlkvdtgk
     2881 kllidsddel rpeeknaeds pdtntstdnq ecndkevsns eqmepqvsse eecegeipkm
     2941 etedtktlle penevpsskd vddkesddmd ednnqksshg snekvskthk hsskeghrrs
     3001 kgkkaparsk iadltsdseg sededktelq nkskivksvf grvfggekad kvkevlndwv
     3061 skseddsdas rsdfrsttpn elnaeetlea rneekrhlvt svsstikkkp grkpkdsssi
     3121 drtkeknpls enreelrgnr hsksrnssgn rkkpeeeasd yyflvlppnk srhskkiaee
     3181 risrafdgvr mfeeflggdq kegnyglrsq skpdltikds gygsfnyded sskmvcseep
     3241 leeeqkllkk rkslssktsk sktdsddwkd lvmemrslks skeptdeedn svkdipeqep
     3301 lssrlsadlk srscsslaps svrnrspsmd rnsqstrdtd vdeededigr grisplfvre
     3361 tpgssiesss nseneedden vlgnestvrk rsssefsgek ivirspssth kgevvtiapt
     3421 daiednaldv pqeietakpr qgkvlnfdee lfveccsrlk attenelrga kkikldhneg
     3481 yhrkdeqqqg lrgprdrwrd vesqnslgsl lesvnqfnef qllgeemyst rerdypkrag
     3541 rnlrsehssr saspdmsrad nlgyedsldv afehnnklrd kiqqrmrese nliastfgks
     3601 sndiahhnkn neqdlrnsys hlqdqgqvsg aslsnrsysd avhirnlqeq qnaakmhlds
     3661 gfknkmnssl gglldkalsn llhsngkhdh ngstpmkvla elacarapts ttglpisqel
     3721 vqpaktspnk esipdpcpvt vpkdiqkkpr npikelferk kemnerkqqe kskteaalre
     3781 lnvhrqrktk kmkkhqdfpl irrgehgggl gerkkrrdgf erkeefvsdr ikdvydfdee
     3841 esqiepnlgs vmsyrsrpgy evsclrtkev dvaglmskai geslengkag dalssrlesm
     3901 idrkfkelek fapktkgalk sfqsedqrqi tgpmdefver kqlkskrpve qslkhsklkk
     3961 rnknpkkktr nawyendssd eyrttavkpe digvgisksq rtcskgkqni faelytsses
     4021 efehdgvnye skkqrrakkg akkvaesedl kqnqesvnky ssenedrend dwrdqdlkdd
     4081 vkhesdhkks esemsdhplv iderkdgedq rnsdeetetq yernyemddl yredssivds
     4141 dvedsvttea qnvpdesrvk nsnslqekin gktncirdel ipleealdml dqadneasea
     4201 nynvdrnvns ledgdpvita epdisetfnp veesavksps lleeqdqele qeeepdndll
     4261 slpeklssne kpqkesdnlp lhvflsrkvq eskkrkeqql kkmqeeqeri lmdfqptrrq
     4321 rkcaigkqgl laeisssdee isprdrrsnd ksdhdkprkq kreskekrke ryiekkheqm
     4381 iakeqkaiee eilrevgkkk dslamnpqnc ksdlsdahdp eklesmedki dqevmqkkkh
     4441 qakekqkkqs kvdeeinvkh nneglddpeg ncnksilaes nhedtnnqes pvkqkrhsks
     4501 ptkpkrtskg dakvaskrnr nansdksrnr adskgsrdke rrssngkhds ddeelkttks
     4561 wnkveegvgv aigrrkraaa nqlyywssss deeemlevvp vveeeeddrq eqhgwivgds
     4621 hkrmitmlam ekqlkekrrr sedefepgra kskkhrnsts
//
LOCUS       XP_034185318             974 aa            linear   INV 03-SEP-2025
DEFINITION  opa1 mitochondrial dynamin like GTPase [Osmia lignaria lignaria].
ACCESSION   XP_034185318
VERSION     XP_034185318.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329427.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..974
                     /product="opa1 mitochondrial dynamin like GTPase"
                     /calculated_mol_wt=113272
     Region          201..>326
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     Region          301..575
                     /region_name="DLP_1"
                     /note="Dynamin_like protein family includes dynamins and
                     Mx proteins; cd08771"
                     /db_xref="CDD:206738"
     Site            309..316
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206738"
     Site            order(310,312..317,330..331,337,412,482,484..485,516..520,
                     523)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206738"
     Site            order(311..312,416,429,454,456,458..461,463..464,485,
                     487..490,519..521)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:206738"
     Site            337
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206738"
     Site            341..343
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206738"
     Site            412..415
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206738"
     Site            order(414..415,445..446)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206738"
     Site            481..484
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206738"
     Site            516..518
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206738"
     Region          631..974
                     /region_name="OPA1_C"
                     /note="Dynamin-like GTPase OPA1 C-terminal; pfam19434"
                     /db_xref="CDD:466080"
     CDS             1..974
                     /gene="Opa1"
                     /coded_by="XM_034329427.2:244..3168"
                     /db_xref="GeneID:117606668"
ORIGIN      
        1 mkqvicrkig driilvskts rypvpivstr nlmsgrynri yrpllasphv ryfgnpnrty
       61 amfigrvlrg alkirylllg gavgggvtlq kkyeqlkevm pdmgwlddil pdekrwknih
      121 nkllsakgil aekmefdpri kefgeakyrq yrewfnqrld daikaaevnq nqavdssdsi
      181 fdatsailyr lyseskdqir tnsvafarpl pdeniqedrk kaqtsqerin amqeevmqtq
      241 lryqrelerl erenkelrkq mllrgnqkln nkkikkslid mysdvldels dydstysttd
      301 hlprvvvvgd qssgktsvle miaqarifpr gggemmtrap vkvtlsegpy hiaqfkdssr
      361 efdltkesel aelrrevelr mknsvkngkt vsqdvismtv kgpglqrmvl vdlpgiistv
      421 tvdmaedtrd airqmtqqhm snpnaiilci qdgsvdaers nvtdlvaqmd ptgkrtifvl
      481 tkvdlaeenl tnpdrlrkil sgklfpmkal gyfavvtgrg kqddsiqtir dyeeqffrts
      541 klfkdglvms gqvttrnlsl avaecfwkmv retveqqadv fkatrfnlet ewknnfprlr
      601 qldrdelfek argeildeiv nlsqvspkhw eetllniiwd kvsthvfeni ylpaaqsgnp
      661 gtfnttvdik lrqwpeehlp arsvesgwec lqmefnhfmn qaklspdhdd ifdnlknavv
      721 tealkrhtwe ekasemlrvi qlntvedksv ndkrewdqav rfletavkek lqnielilre
      781 mlgpgrkerw lywqsqsdeq rqrsavknel dkilyadkkh sptltqdeit tvrknlqrng
      841 leidnefire twhpvyrryf lqqslakayd ckkgyylyht ghesevdcnd vvlfwriqqv
      901 lkvtanalrq qimnrearrl dkeikevled ysqdneikkq lltgrrvtla eelervrqiq
      961 ekleefiqal kkek
//
LOCUS       XP_034185319             718 aa            linear   INV 03-SEP-2025
DEFINITION  BBSome complex member BBS7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185319
VERSION     XP_034185319.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329428.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..718
                     /product="BBSome complex member BBS7 isoform X1"
                     /calculated_mol_wt=79814
     CDS             1..718
                     /gene="LOC117606669"
                     /coded_by="XM_034329428.2:1..2157"
                     /db_xref="GeneID:117606669"
ORIGIN      
        1 mtlplsridy ttvgvtsrgt mkifpasehr qtqkfavadh dgilyvygik kgelqlsfks
       61 lpgprinkvv lggptrdrif iaqdstvkgy tkkgklflef dtnlidpits lyvlgpnlaa
      121 cardlyhryh dckdadsyla geilydvill padatfiyai lacgdcairv lhgtktpavl
      181 rlpsvptvls mcrerdaesk ehmlvgtmdg kvgllilesn ktlriswiln mtgsevtsld
      241 tyelqdgldi ligrqdgtie vytfpeedtl sllryrcnan esitsvrggi igaagypevl
      301 vttysgkifg lttrppglle adqndglmkl kaeiqllqek lneekeatnf tvdplaplil
      361 svnhriilnr edasyslsve ldtpidnili qsdtpielld vesnsavvsl stcnpregnf
      421 vlatyrcqvn tnhlemklrt iegqsgtlqi yvtsqvqpkc crrisipvca lslhvrlhen
      481 dgtevhergp fnelkvvgsf tiaemhawls lalldvperp hleegeavlt yissfigtvl
      541 kckykkgsav flgenvssii ilrdvltrea tkrkikldvf cdvaegsvsk vlelilprln
      601 gaheltrkvk ilnaleewel kenpkdnlcs eyqelmqket eiksqmakds eilerlqavi
      661 tdlyvdwera krsrritskd aaeklsaale srdlvqllqi fintnntvpa paslssim
//
LOCUS       XP_034185320             689 aa            linear   INV 03-SEP-2025
DEFINITION  BBSome complex member BBS7 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185320
VERSION     XP_034185320.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329429.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..689
                     /product="BBSome complex member BBS7 isoform X2"
                     /calculated_mol_wt=76508
     CDS             1..689
                     /gene="LOC117606669"
                     /coded_by="XM_034329429.2:61..2130"
                     /db_xref="GeneID:117606669"
ORIGIN      
        1 mtlplsridy ttvgvtsrgi kkgelqlsfk slpgprinkv vlggptrdri fiaqdstvkg
       61 ytkkgklfle fdtnlidpit slyvlgpnla acardlyhry hdckdadsyl ageilydvil
      121 lpadatfiya ilacgdcair vlhgtktpav lrlpsvptvl smcrerdaes kehmlvgtmd
      181 gkvglliles nktlriswil nmtgsevtsl dtyelqdgld iligrqdgti evytfpeedt
      241 lsllryrcna nesitsvrgg iigaagypev lvttysgkif glttrppgll eadqndglmk
      301 lkaeiqllqe klneekeatn ftvdplapli lsvnhriiln redasyslsv eldtpidnil
      361 iqsdtpiell dvesnsavvs lstcnpregn fvlatyrcqv ntnhlemklr tiegqsgtlq
      421 iyvtsqvqpk ccrrisipvc alslhvrlhe ndgtevherg pfnelkvvgs ftiaemhawl
      481 slalldvper phleegeavl tyissfigtv lkckykkgsa vflgenvssi iilrdvltre
      541 atkrkikldv fcdvaegsvs kvlelilprl ngaheltrkv kilnaleewe lkenpkdnlc
      601 seyqelmqke teiksqmakd seilerlqav itdlyvdwer akrsrritsk daaeklsaal
      661 esrdlvqllq ifintnntvp apaslssim
//
LOCUS       XP_034185321             648 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606670 [Osmia lignaria lignaria].
ACCESSION   XP_034185321
VERSION     XP_034185321.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329430.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..648
                     /product="uncharacterized protein LOC117606670"
                     /calculated_mol_wt=73520
     Region          <385..543
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          386..406
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          414..434
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          442..463
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          471..491
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(476,478,480,482..483,486..487,490,504,506,510..511,
                     514..515,518,532,534,536,538..539,542..543,546)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          499..519
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          527..547
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..648
                     /gene="LOC117606670"
                     /coded_by="XM_034329430.2:244..2190"
                     /db_xref="GeneID:117606670"
ORIGIN      
        1 mevtteeaqa vllegmegaq yitiqradge plsksvpllt lngelipegm vvdminagvg
       61 tdygtatqyy eaddllqhel teedrrlaaa lvavqlqqqq kqqqlhsqsq hedstlpdvs
      121 hletltpvnt ysiqtvpepn appvyptlqp fkrithnvkq efinvkseyd vdvsaessed
      181 atpssgpkrs lphkkriprn fkqqtkrnst rkdssrlnsd ginaesstdt qahvfkcelc
      241 sskfssqlkf fehlkvhyep vkqetriaqa tntnitisvp etvqslentv ieefsepedl
      301 megirgvvee tgahiddete splstvnnes plwtitdvte hvhrdhvkpa tvneqtlhnk
      361 ektdipqtmk kkkilkarks ndelmcsqcd rtfhhknslv yhmrshsger phqcevcgks
      421 ffapsalkvh krlhsgdkpy kceecgrhfr qwgdlkyhst sihseqkqyq ceycgkdfar
      481 kyslivhrri htgeknyrce fcnktfrass ylqnhrriht gekphqcavc gkpfrvrsdm
      541 krhmhthsrg rtdrplnrvi sgksiddend kgsqaktiqd lvrdlkleve atgvveivpp
      601 ddnpesilph aggqtleytv tastdgnsdr dpleavvrtt dnmqyffm
//
LOCUS       XP_034185323             420 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 184C [Osmia lignaria lignaria].
ACCESSION   XP_034185323
VERSION     XP_034185323.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329432.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..420
                     /product="transmembrane protein 184C"
                     /calculated_mol_wt=47968
     Region          48..315
                     /region_name="Solute_trans_a"
                     /note="Organic solute transporter Ostalpha; pfam03619"
                     /db_xref="CDD:460991"
     CDS             1..420
                     /gene="LOC117606671"
                     /coded_by="XM_034329432.2:231..1493"
                     /db_xref="GeneID:117606671"
ORIGIN      
        1 masicrrwrl wilpvltcly alliiilvpi llansikngf rkqdqgalvg gafvllalpi
       61 afyeivqhmi yytqprlqky iirilwmvpi yavnawlglv ypegsiyvds lrecyeayvi
      121 ynfmmyllay ldadrqlehr lemspqvhhm fplcclpdwe mgrefvhmck hgilqytavr
      181 pittlmsfic elngvygege frtdvafpym ialnnlsqfv amyclvlfyr anaealkpmk
      241 pigkflcika vvffsffqgv ivallvyfdv issifntndm ddirnisskl qdfliciemf
      301 laavahhysf tykpfvnlaq gqawwdafra mwdvsdvhnd ikehlgvvgs slsrrirgrs
      361 ayqqawgsat ertsllpeas vtttrsapac sfyntgeidq ndapvdlvpd vqdttnavkt
//
LOCUS       XP_034185324             420 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 184C [Osmia lignaria lignaria].
ACCESSION   XP_034185324
VERSION     XP_034185324.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329433.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..420
                     /product="transmembrane protein 184C"
                     /calculated_mol_wt=47968
     Region          48..315
                     /region_name="Solute_trans_a"
                     /note="Organic solute transporter Ostalpha; pfam03619"
                     /db_xref="CDD:460991"
     CDS             1..420
                     /gene="LOC117606671"
                     /coded_by="XM_034329433.2:322..1584"
                     /db_xref="GeneID:117606671"
ORIGIN      
        1 masicrrwrl wilpvltcly alliiilvpi llansikngf rkqdqgalvg gafvllalpi
       61 afyeivqhmi yytqprlqky iirilwmvpi yavnawlglv ypegsiyvds lrecyeayvi
      121 ynfmmyllay ldadrqlehr lemspqvhhm fplcclpdwe mgrefvhmck hgilqytavr
      181 pittlmsfic elngvygege frtdvafpym ialnnlsqfv amyclvlfyr anaealkpmk
      241 pigkflcika vvffsffqgv ivallvyfdv issifntndm ddirnisskl qdfliciemf
      301 laavahhysf tykpfvnlaq gqawwdafra mwdvsdvhnd ikehlgvvgs slsrrirgrs
      361 ayqqawgsat ertsllpeas vtttrsapac sfyntgeidq ndapvdlvpd vqdttnavkt
//
LOCUS       XP_034185325             420 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 184C [Osmia lignaria lignaria].
ACCESSION   XP_034185325
VERSION     XP_034185325.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..420
                     /product="transmembrane protein 184C"
                     /calculated_mol_wt=47968
     Region          48..315
                     /region_name="Solute_trans_a"
                     /note="Organic solute transporter Ostalpha; pfam03619"
                     /db_xref="CDD:460991"
     CDS             1..420
                     /gene="LOC117606671"
                     /coded_by="XM_034329434.2:326..1588"
                     /db_xref="GeneID:117606671"
ORIGIN      
        1 masicrrwrl wilpvltcly alliiilvpi llansikngf rkqdqgalvg gafvllalpi
       61 afyeivqhmi yytqprlqky iirilwmvpi yavnawlglv ypegsiyvds lrecyeayvi
      121 ynfmmyllay ldadrqlehr lemspqvhhm fplcclpdwe mgrefvhmck hgilqytavr
      181 pittlmsfic elngvygege frtdvafpym ialnnlsqfv amyclvlfyr anaealkpmk
      241 pigkflcika vvffsffqgv ivallvyfdv issifntndm ddirnisskl qdfliciemf
      301 laavahhysf tykpfvnlaq gqawwdafra mwdvsdvhnd ikehlgvvgs slsrrirgrs
      361 ayqqawgsat ertsllpeas vtttrsapac sfyntgeidq ndapvdlvpd vqdttnavkt
//
LOCUS       XP_034185326             199 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606672 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185326
VERSION     XP_034185326.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329435.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="uncharacterized protein LOC117606672 isoform X1"
                     /calculated_mol_wt=22268
     Region          123..175
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..199
                     /gene="LOC117606672"
                     /coded_by="XM_034329435.2:128..727"
                     /db_xref="GeneID:117606672"
ORIGIN      
        1 mfylqitilc mamvhvicdl ppgtrrntyl ppeptkgyny dtpsipfprp sttrppfsst
       61 qrypspkptt kfpprptpse gypppgprpt pefpdygsst rptgntitps ghdhhhhepg
      121 mpfdfnyavk edafgndysh naisdgdivr geyrvqlpdg rlqivrytad wkhgfsaqvs
      181 ydgnprfdvp rptsnfagy
//
LOCUS       XP_034185327             199 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606672 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185327
VERSION     XP_034185327.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329436.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="uncharacterized protein LOC117606672 isoform X1"
                     /calculated_mol_wt=22268
     Region          123..175
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..199
                     /gene="LOC117606672"
                     /coded_by="XM_034329436.2:153..752"
                     /db_xref="GeneID:117606672"
ORIGIN      
        1 mfylqitilc mamvhvicdl ppgtrrntyl ppeptkgyny dtpsipfprp sttrppfsst
       61 qrypspkptt kfpprptpse gypppgprpt pefpdygsst rptgntitps ghdhhhhepg
      121 mpfdfnyavk edafgndysh naisdgdivr geyrvqlpdg rlqivrytad wkhgfsaqvs
      181 ydgnprfdvp rptsnfagy
//
LOCUS       XP_034185328             199 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606672 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185328
VERSION     XP_034185328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="uncharacterized protein LOC117606672 isoform X1"
                     /calculated_mol_wt=22268
     Region          123..175
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..199
                     /gene="LOC117606672"
                     /coded_by="XM_034329437.2:149..748"
                     /db_xref="GeneID:117606672"
ORIGIN      
        1 mfylqitilc mamvhvicdl ppgtrrntyl ppeptkgyny dtpsipfprp sttrppfsst
       61 qrypspkptt kfpprptpse gypppgprpt pefpdygsst rptgntitps ghdhhhhepg
      121 mpfdfnyavk edafgndysh naisdgdivr geyrvqlpdg rlqivrytad wkhgfsaqvs
      181 ydgnprfdvp rptsnfagy
//
LOCUS       XP_034185329             196 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606672 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185329
VERSION     XP_034185329.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329438.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="uncharacterized protein LOC117606672 isoform X2"
                     /calculated_mol_wt=21773
     Region          120..172
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..196
                     /gene="LOC117606672"
                     /coded_by="XM_034329438.2:402..992"
                     /db_xref="GeneID:117606672"
ORIGIN      
        1 mgitilcmam vhvicdlppg trrntylppe ptkgynydtp sipfprpstt rppfsstqry
       61 pspkpttkfp prptpsegyp ppgprptpef pdygsstrpt gntitpsghd hhhhepgmpf
      121 dfnyavkeda fgndyshnai sdgdivrgey rvqlpdgrlq ivrytadwkh gfsaqvsydg
      181 nprfdvprpt snfagy
//
LOCUS       XP_034185361            1409 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606690 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185361
VERSION     XP_034185361.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329470.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185361.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1409
                     /product="uncharacterized protein LOC117606690 isoform X1"
                     /calculated_mol_wt=161825
     Region          598..763
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..1409
                     /gene="LOC117606690"
                     /coded_by="XM_034329470.2:348..4577"
                     /db_xref="GeneID:117606690"
ORIGIN      
        1 mdhnlfrdyc kaensddeea nfdlqsrlya eiyygpkdie smdvkpviks etvstdneal
       61 qrkesiittp npsdntkkdn ydtnhrhsen sstiidnksn ikaivtptqt epvyggtgsq
      121 nnlsissikt lehsignigs ikndadsmmc hnvknaktsl dhqnvktern vaepevetes
      181 keqlslklln pktpvqsscn eekkevtcte rnsnensdni ltkyefakli inklylrkyv
      241 dleerlqeie kekemekekd kerekeeclk qaelkereea elkereeael kereeaklke
      301 seekrkqqqt rnstnvvdea dtdsrykvqt knekpqktpd cceeivllse tdsnsedsil
      361 evpippkplp pvinlndsde ssesssdsds nvdpfivehk ndtskitddk hksdkssvin
      421 tvtedimlnc tevqkcassi eeireasksv qsqpisnetq lgilyypfsd dentqdihlp
      481 sistansnvv yerdknesin faedvplsnf vnvspkests nkrrrdetdn etgnnaeqne
      541 ntgneskrtk lspkkppnnd knkkqtweey ffaplsdnlk afynesrgqe nfdireiqnk
      601 msrdprlwti ldedlipdla rqqrywnskc nycqfeghkr rncpeltkpp rchmcgsegh
      661 tesrcpekmc ltcgkkqgtf rktcescrtl ycnmcnavgh kstecpdlwr rfhqttqnce
      721 ieipesisdv mkppdllycc nctkrghdss tcneyrwsqh fptpayvsny vdgpkcenap
      781 pavssksedv iplaaktkkh kymsflqgqe nldgcfvvys ygafyikepk geevkrnlvg
      841 dqihasqltn flkgrivpaf ldqlkkiikf eikiyydsqr slmirirsmv nvapnlcdif
      901 vfwlklsded khlmiytdfp yskrkmanfl ktklnalrkd ldhpeslhqq ikqlkeeipn
      961 vrdprervsm skqlmslqek ftrafhampk qsaevkrlrk cikylntstq hqvemslyfn
     1021 imyvynkifl prtltnaelt rflkkyqrsv ntqirhnqky mekkneeckk qkkqkpnpye
     1081 sfvqkitnan eskgktntcn itdtppkpsa yqtfvqeirn ynelksktna cnindaaqst
     1141 kssekhnkqt stvisttveh pvvtdtvqyd nvtegsignt rvenfnmean inesstvypi
     1201 erislprdhl rrkdsevidt vknystapss kprpmvtrsm skdtavrpnl itipientgs
     1261 lmkhskkkhs eieppqnsge ksseqtsdss kvmdtsyseq ddaansdana tkkkkskksk
     1321 kaktdlddsl qvtedqatds dgnmvnkaqt videalafsl phmnkaaeev qkrisnnnlk
     1381 qehinvlqrl inlekehqqy vssfcnylq
//
LOCUS       XP_034185362            1400 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606690 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185362
VERSION     XP_034185362.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329471.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185362.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1400
                     /product="uncharacterized protein LOC117606690 isoform X2"
                     /calculated_mol_wt=160672
     Region          <613..754
                     /region_name="AIR1"
                     /note="Arginine methyltransferase-interacting protein,
                     contains RING Zn-finger [Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5082"
                     /db_xref="CDD:227414"
     CDS             1..1400
                     /gene="LOC117606690"
                     /coded_by="XM_034329471.2:348..4550"
                     /db_xref="GeneID:117606690"
ORIGIN      
        1 mdhnlfrdyc kaensddeea nfdlqsrlya eiyygpkdie smdvkpviks etvstdneal
       61 qrkesiittp npsdntkkdn ydtnhrhsen sstiidnksn ikaivtptqt epvyggtgsq
      121 nnlsissikt lehsignigs ikndadsmmc hnvknaktsl dhqnvktern vaepevetes
      181 keqlslklln pktpvqsscn eekkevtcte rnsnensdni ltkyefakli inklylrkyv
      241 dleerlqeie kekemekekd kerekeeclk qaelkereea elkereeael kereeaklke
      301 seekrkqqqt rnstnvvdea dtdsrykvqt knekpqktpd cceeivllse tdsnsedsil
      361 evpippkplp pvinlndsde ssesssdsds nvdpfivehk ndtskitddk hksdkssvin
      421 tvtedimlnc tevqkcassi eeireasksv qsqpisnetq lgilyypfsd dentqdihlp
      481 sistansnvv yerdknesin faedvplsnf vnvspkests nkrrrdetdn etgnnaeqne
      541 ntgneskrtk lspkkppnnd knkkqtweey ffaplsdnlk afynesrgqe nfdireiqnk
      601 msnedlipdl arqqrywnsk cnycqfeghk rrncpeltkp prchmcgseg htesrcpekm
      661 cltcgkkqgt frktcescrt lycnmcnavg hkstecpdlw rrfhqttqnc eieipesisd
      721 vmkppdllyc cnctkrghds stcneyrwsq hfptpayvsn yvdgpkcena ppavssksed
      781 viplaaktkk hkymsflqgq enldgcfvvy sygafyikep kgeevkrnlv gdqihasqlt
      841 nflkgrivpa fldqlkkiik feikiyydsq rslmirirsm vnvapnlcdi fvfwlklsde
      901 dkhlmiytdf pyskrkmanf lktklnalrk dldhpeslhq qikqlkeeip nvrdprervs
      961 mskqlmslqe kftrafhamp kqsaevkrlr kcikylntst qhqvemslyf nimyvynkif
     1021 lprtltnael trflkkyqrs vntqirhnqk ymekkneeck kqkkqkpnpy esfvqkitna
     1081 neskgktntc nitdtppkps ayqtfvqeir nynelksktn acnindaaqs tkssekhnkq
     1141 tstvisttve hpvvtdtvqy dnvtegsign trvenfnmea ninesstvyp ierislprdh
     1201 lrrkdsevid tvknystaps skprpmvtrs mskdtavrpn litipientg slmkhskkkh
     1261 seieppqnsg eksseqtsds skvmdtsyse qddaansdan atkkkkskks kkaktdldds
     1321 lqvtedqatd sdgnmvnkaq tvidealafs lphmnkaaee vqkrisnnnl kqehinvlqr
     1381 linlekehqq yvssfcnylq
//
LOCUS       XP_034185363             592 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606691 [Osmia lignaria lignaria].
ACCESSION   XP_034185363
VERSION     XP_034185363.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329472.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185363.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..592
                     /product="uncharacterized protein LOC117606691"
                     /calculated_mol_wt=64678
     CDS             1..592
                     /gene="LOC117606691"
                     /coded_by="XM_034329472.2:576..2354"
                     /db_xref="GeneID:117606691"
ORIGIN      
        1 mspasqggve veryigscli ssnirggsqk iagkkqepvi rgksrscygg qnfansteqc
       61 qvqygaggwt gaplismtss prrcnqqqqq qqqqqqqqsq qvcdqqvsvh lhknplsrla
      121 ihtqgaplil agrfhnrgkv htngtanavg ntvcgnaavn syvhntgntg tmnagvrcpp
      181 arpqrsmels kkqqgnpkse naaagnsian idslsiasde ssgsnnsens lpriikprkr
      241 rkkdrkpann vaistdvakh eeqqhqsggs vasseqsnvv alkpyvpacy epryeavpah
      301 lrshrrpglg regasmygms mqemvdcrqr yahrhaetkv lddnrnvvmp gqmrailpkd
      361 ytrhngppry lhedgvkdgl gscqcrycdp saliwdvdqn cyspfltpsp stefcpshys
      421 qmplflsrps lncpeqnidr lfnvdaarlq ekdscgsngv vlrrswsdpt syfseeiaap
      481 srdvgvigdr gqaesgkrrt lwrgdtigvp tgnvgsatat gmnlaspkgl evsteiitsp
      541 nghrdleikf ysaspnaapt eeadededfs diwsyheskl qqdfrtllqa ee
//
LOCUS       XP_034185364             553 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185364
VERSION     XP_034185364.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329473.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X1"
                     /calculated_mol_wt=62896
     Region          <42..>142
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          85..107
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          108..130
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          131..142
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          195..539
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,
                     489..490,530)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,489,530)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,415,417,490,529..530)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(199,244,529)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(364,417,530)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(415,417,530)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..553
                     /gene="twin"
                     /coded_by="XM_034329473.2:202..1863"
                     /db_xref="GeneID:117606692"
ORIGIN      
        1 msrnhkdkye nsnqvlpilp rrahtfmste dansgkksyw peleitgsir nlspnlwqlt
       61 hltalylndn slqripseig rlvnlraldl ssnklrslpa elgdliylre lllnqnylrv
      121 lpyelgklfq lqvlglqgnp lskevmalyg epsgthklls ymldnlqvta nhppqrpwip
      181 ltrpnrtrpt ciftvmcynv lcdkyatrqm ygycpswald weyrkkgild eirhyaadii
      241 slqevetdqf ynfflpelkq dgydgifspk sraktmaend rkyvdgcaif yrtakftlik
      301 ehlvefnqla manaegsdnm lnrvmpkdni glaallrtke aawdnglpsd paqvqqpilv
      361 ctahihwdpe fcdvkliqtm mlsnelrsil dqagqsfrpg hkpdssnvql llcgdfnslp
      421 dsgvieflts grvaadhrdf kdlayksclq kisgcdkpne fthsfklasa ysedimpytn
      481 ytfefkgiid yifyskqsmv plgllgplsa dwfkdhkvvg cphphvpsdh fpllvelemt
      541 ptvgtsngli srr
//
LOCUS       XP_034185365             553 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185365
VERSION     XP_034185365.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329474.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X1"
                     /calculated_mol_wt=62896
     Region          <42..>142
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          85..107
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          108..130
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          131..142
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          195..539
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,
                     489..490,530)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,489,530)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,415,417,490,529..530)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(199,244,529)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(364,417,530)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(415,417,530)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..553
                     /gene="twin"
                     /coded_by="XM_034329474.2:160..1821"
                     /db_xref="GeneID:117606692"
ORIGIN      
        1 msrnhkdkye nsnqvlpilp rrahtfmste dansgkksyw peleitgsir nlspnlwqlt
       61 hltalylndn slqripseig rlvnlraldl ssnklrslpa elgdliylre lllnqnylrv
      121 lpyelgklfq lqvlglqgnp lskevmalyg epsgthklls ymldnlqvta nhppqrpwip
      181 ltrpnrtrpt ciftvmcynv lcdkyatrqm ygycpswald weyrkkgild eirhyaadii
      241 slqevetdqf ynfflpelkq dgydgifspk sraktmaend rkyvdgcaif yrtakftlik
      301 ehlvefnqla manaegsdnm lnrvmpkdni glaallrtke aawdnglpsd paqvqqpilv
      361 ctahihwdpe fcdvkliqtm mlsnelrsil dqagqsfrpg hkpdssnvql llcgdfnslp
      421 dsgvieflts grvaadhrdf kdlayksclq kisgcdkpne fthsfklasa ysedimpytn
      481 ytfefkgiid yifyskqsmv plgllgplsa dwfkdhkvvg cphphvpsdh fpllvelemt
      541 ptvgtsngli srr
//
LOCUS       XP_034185366             553 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185366
VERSION     XP_034185366.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329475.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X1"
                     /calculated_mol_wt=62896
     Region          <42..>142
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          85..107
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          108..130
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          131..142
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          195..539
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,
                     489..490,530)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,489,530)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,415,417,490,529..530)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(199,244,529)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(364,417,530)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(415,417,530)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..553
                     /gene="twin"
                     /coded_by="XM_034329475.2:136..1797"
                     /db_xref="GeneID:117606692"
ORIGIN      
        1 msrnhkdkye nsnqvlpilp rrahtfmste dansgkksyw peleitgsir nlspnlwqlt
       61 hltalylndn slqripseig rlvnlraldl ssnklrslpa elgdliylre lllnqnylrv
      121 lpyelgklfq lqvlglqgnp lskevmalyg epsgthklls ymldnlqvta nhppqrpwip
      181 ltrpnrtrpt ciftvmcynv lcdkyatrqm ygycpswald weyrkkgild eirhyaadii
      241 slqevetdqf ynfflpelkq dgydgifspk sraktmaend rkyvdgcaif yrtakftlik
      301 ehlvefnqla manaegsdnm lnrvmpkdni glaallrtke aawdnglpsd paqvqqpilv
      361 ctahihwdpe fcdvkliqtm mlsnelrsil dqagqsfrpg hkpdssnvql llcgdfnslp
      421 dsgvieflts grvaadhrdf kdlayksclq kisgcdkpne fthsfklasa ysedimpytn
      481 ytfefkgiid yifyskqsmv plgllgplsa dwfkdhkvvg cphphvpsdh fpllvelemt
      541 ptvgtsngli srr
//
LOCUS       XP_034185367             533 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034185367
VERSION     XP_034185367.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329476.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X2"
                     /calculated_mol_wt=60663
     Region          <42..>142
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          85..107
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          108..130
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          131..142
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          195..528
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,
                     489..490)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(199,244,364,367,369,375,415,417,419,480,485,489)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     CDS             1..533
                     /gene="twin"
                     /coded_by="XM_034329476.1:204..1805"
                     /db_xref="GeneID:117606692"
ORIGIN      
        1 msrnhkdkye nsnqvlpilp rrahtfmste dansgkksyw peleitgsir nlspnlwqlt
       61 hltalylndn slqripseig rlvnlraldl ssnklrslpa elgdliylre lllnqnylrv
      121 lpyelgklfq lqvlglqgnp lskevmalyg epsgthklls ymldnlqvta nhppqrpwip
      181 ltrpnrtrpt ciftvmcynv lcdkyatrqm ygycpswald weyrkkgild eirhyaadii
      241 slqevetdqf ynfflpelkq dgydgifspk sraktmaend rkyvdgcaif yrtakftlik
      301 ehlvefnqla manaegsdnm lnrvmpkdni glaallrtke aawdnglpsd paqvqqpilv
      361 ctahihwdpe fcdvkliqtm mlsnelrsil dqagqsfrpg hkpdssnvql llcgdfnslp
      421 dsgvieflts grvaadhrdf kdlayksclq kisgcdkpne fthsfklasa ysedimpytn
      481 ytfefkgiid yifyskqsmv plgllgplsa dwfkdhkvvg cphphvpsef vgv
//
LOCUS       XP_034185368             423 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034185368
VERSION     XP_034185368.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329477.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..423
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X3"
                     /calculated_mol_wt=47699
     Region          65..409
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(69,114,234,237,239,245,285,287,289,350,355,359..360,
                     400)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(69,114,234,237,239,245,285,287,289,350,355,359,400)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(69,114,234,285,287,360,399..400)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(69,114,399)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(234,287,400)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(285,287,400)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..423
                     /gene="twin"
                     /coded_by="XM_034329477.2:214..1485"
                     /db_xref="GeneID:117606692"
ORIGIN      
        1 meevmavtvi sgesltpfva asssyilrfl sisavakita nhppqrpwip ltrpnrtrpt
       61 ciftvmcynv lcdkyatrqm ygycpswald weyrkkgild eirhyaadii slqevetdqf
      121 ynfflpelkq dgydgifspk sraktmaend rkyvdgcaif yrtakftlik ehlvefnqla
      181 manaegsdnm lnrvmpkdni glaallrtke aawdnglpsd paqvqqpilv ctahihwdpe
      241 fcdvkliqtm mlsnelrsil dqagqsfrpg hkpdssnvql llcgdfnslp dsgvieflts
      301 grvaadhrdf kdlayksclq kisgcdkpne fthsfklasa ysedimpytn ytfefkgiid
      361 yifyskqsmv plgllgplsa dwfkdhkvvg cphphvpsdh fpllvelemt ptvgtsngli
      421 srr
//
LOCUS       XP_034185369             358 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034185369
VERSION     XP_034185369.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329478.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X4"
                     /calculated_mol_wt=40545
     Region          1..344
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(4,49,169,172,174,180,220,222,224,285,290,294..295,
                     335)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(4,49,169,172,174,180,220,222,224,285,290,294,335)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(4,49,169,220,222,295,334..335)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(4,49,334)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(169,222,335)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(220,222,335)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..358
                     /gene="twin"
                     /coded_by="XM_034329478.2:454..1530"
                     /db_xref="GeneID:117606692"
ORIGIN      
        1 mcynvlcdky atrqmygycp swaldweyrk kgildeirhy aadiislqev etdqfynffl
       61 pelkqdgydg ifspksrakt maendrkyvd gcaifyrtak ftlikehlve fnqlamanae
      121 gsdnmlnrvm pkdniglaal lrtkeaawdn glpsdpaqvq qpilvctahi hwdpefcdvk
      181 liqtmmlsne lrsildqagq sfrpghkpds snvqlllcgd fnslpdsgvi efltsgrvaa
      241 dhrdfkdlay ksclqkisgc dkpnefthsf klasaysedi mpytnytfef kgiidyifys
      301 kqsmvplgll gplsadwfkd hkvvgcphph vpsdhfpllv elemtptvgt snglisrr
//
LOCUS       XP_076545754             358 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 6-like twin isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_076545754
VERSION     XP_076545754.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689639.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="CCR4-NOT transcription complex subunit 6-like
                     twin isoform X4"
                     /calculated_mol_wt=40545
     Region          1..344
                     /region_name="Deadenylase_CCR4"
                     /note="C-terminal deadenylase domain of CCR4 and related
                     domains; cd09097"
                     /db_xref="CDD:197331"
     Site            order(4,49,169,172,174,180,220,222,224,285,290,294..295,
                     335)
                     /site_type="active"
                     /db_xref="CDD:197331"
     Site            order(4,49,169,172,174,180,220,222,224,285,290,294,335)
                     /site_type="other"
                     /note="poly(A) RNA binding site [nucleotide binding]"
                     /db_xref="CDD:197331"
     Site            order(4,49,169,220,222,295,334..335)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(4,49,334)
                     /site_type="other"
                     /note="Mg binding site A [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(169,222,335)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(220,222,335)
                     /site_type="other"
                     /note="Mg binding site B [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..358
                     /gene="twin"
                     /coded_by="XM_076689639.1:103..1179"
                     /db_xref="GeneID:117606692"
ORIGIN      
        1 mcynvlcdky atrqmygycp swaldweyrk kgildeirhy aadiislqev etdqfynffl
       61 pelkqdgydg ifspksrakt maendrkyvd gcaifyrtak ftlikehlve fnqlamanae
      121 gsdnmlnrvm pkdniglaal lrtkeaawdn glpsdpaqvq qpilvctahi hwdpefcdvk
      181 liqtmmlsne lrsildqagq sfrpghkpds snvqlllcgd fnslpdsgvi efltsgrvaa
      241 dhrdfkdlay ksclqkisgc dkpnefthsf klasaysedi mpytnytfef kgiidyifys
      301 kqsmvplgll gplsadwfkd hkvvgcphph vpsdhfpllv elemtptvgt snglisrr
//
LOCUS       XP_034184821             274 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184821
VERSION     XP_034184821.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328930.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..274
                     /product="tetraspanin 26A isoform X1"
                     /calculated_mol_wt=30750
     Region          18..191
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          115..238
                     /region_name="TM4SF9_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF9_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03159"
                     /db_xref="CDD:239412"
     Site            order(116,121,125,127..128,131,145,148..149,153..154)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239412"
     CDS             1..274
                     /gene="Tsp26A"
                     /coded_by="XM_034328930.2:462..1286"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mpavrkyrrd tsevscclky vifgfnvmfw wlglgimavg vwawtekdtf nnlsrltnva
       61 ldpafililv gtvtfiigft gcvgalrent cllaayaifl allllmeiav gvlgfvfkdw
      121 iksqatggfq afiihyredp dqqnlidwiq edwlqccgie gpkdwdrnny fncsssdigs
      181 reacgvpfsc ckrkpneiik nkqcgydvrk psynfevakv iyekgciqag eewvqrnlls
      241 iatgavgtaf aqilgicfaq nlradifaqk akwh
//
LOCUS       XP_034184822             272 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184822
VERSION     XP_034184822.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328931.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="tetraspanin 26A isoform X2"
                     /calculated_mol_wt=30615
     Region          18..191
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          115..236
                     /region_name="TM4SF9_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF9_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03159"
                     /db_xref="CDD:239412"
     Site            order(116,121,125,127..128,131,145,148..149,153..154)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239412"
     CDS             1..272
                     /gene="Tsp26A"
                     /coded_by="XM_034328931.2:467..1285"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mpavrkyrrd tsevscclky vifgfnvmfw wlglgimavg vwawtekdtf nnlsrltnva
       61 ldpafililv gtvtfiigft gcvgalrent cllaayaifl allllmeiav gvlgfvfkdw
      121 iksqatggfq afiihyredp dqqnlidwiq edwlqccgie gpkdwdrnny fncsssdigs
      181 reacgvpfsc ckrkpneiik nkqcgydvrk psytgersiy ergclragee wlelnlvpva
      241 gtvvstmvlq ilgicfaqnl radifaqkak wh
//
LOCUS       XP_034184823             254 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034184823
VERSION     XP_034184823.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328932.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="tetraspanin 26A isoform X3"
                     /calculated_mol_wt=28156
     Region          12..242
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          95..218
                     /region_name="TM4SF9_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF9_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03159"
                     /db_xref="CDD:239412"
     Site            order(96,101,105,107..108,111,125,128..129,133..134)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239412"
     CDS             1..254
                     /gene="Tsp26A"
                     /coded_by="XM_034328932.2:664..1428"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mnsstnkntg wlglgimavg vwawtekdtf nnlsrltnva ldpafililv gtvtfiigft
       61 gcvgalrent cllaayaifl allllmeiav gvlgfvfkdw iksqatggfq afiihyredp
      121 dqqnlidwiq edwlqccgie gpkdwdrnny fncsssdigs reacgvpfsc ckrkpneiik
      181 nkqcgydvrk psynfevakv iyekgciqag eewvqrnlls iatgavgtaf aqilgicfaq
      241 nlradifaqk akwh
//
LOCUS       XP_076545755             238 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076545755
VERSION     XP_076545755.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689640.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..238
                     /product="tetraspanin 26A isoform X4"
                     /calculated_mol_wt=26481
     Region          1..226
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          79..202
                     /region_name="TM4SF9_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF9_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03159"
                     /db_xref="CDD:239412"
     Site            order(80,85,89,91..92,95,109,112..113,117..118)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239412"
     CDS             1..238
                     /gene="Tsp26A"
                     /coded_by="XM_076689640.1:536..1252"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mavgvwawte kdtfnnlsrl tnvaldpafi lilvgtvtfi igftgcvgal rentcllaay
       61 aiflallllm eiavgvlgfv fkdwiksqat ggfqafiihy redpdqqnli dwiqedwlqc
      121 cgiegpkdwd rnnyfncsss digsreacgv pfscckrkpn eiiknkqcgy dvrkpsynfe
      181 vakviyekgc iqageewvqr nllsiatgav gtafaqilgi cfaqnlradi faqkakwh
//
LOCUS       XP_076545756             238 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076545756
VERSION     XP_076545756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689641.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..238
                     /product="tetraspanin 26A isoform X4"
                     /calculated_mol_wt=26481
     Region          1..226
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          79..202
                     /region_name="TM4SF9_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF9_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03159"
                     /db_xref="CDD:239412"
     Site            order(80,85,89,91..92,95,109,112..113,117..118)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239412"
     CDS             1..238
                     /gene="Tsp26A"
                     /coded_by="XM_076689641.1:325..1041"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mavgvwawte kdtfnnlsrl tnvaldpafi lilvgtvtfi igftgcvgal rentcllaay
       61 aiflallllm eiavgvlgfv fkdwiksqat ggfqafiihy redpdqqnli dwiqedwlqc
      121 cgiegpkdwd rnnyfncsss digsreacgv pfscckrkpn eiiknkqcgy dvrkpsynfe
      181 vakviyekgc iqageewvqr nllsiatgav gtafaqilgi cfaqnlradi faqkakwh
//
LOCUS       XP_034184825             235 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034184825
VERSION     XP_034184825.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328934.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="tetraspanin 26A isoform X5"
                     /calculated_mol_wt=26555
     Region          18..191
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          115..>210
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cl02781"
                     /db_xref="CDD:351888"
     Site            order(116,121,125,127..128,131,145,148..149,153..154)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239401"
     CDS             1..235
                     /gene="Tsp26A"
                     /coded_by="XM_034328934.2:465..1172"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mpavrkyrrd tsevscclky vifgfnvmfw wlglgimavg vwawtekdtf nnlsrltnva
       61 ldpafililv gtvtfiigft gcvgalrent cllaayaifl allllmeiav gvlgfvfkdw
      121 iksqatggfq afiihyredp dqqnlidwiq edwlqccgie gpkdwdrnny fncsssdigs
      181 reacgvpfsc ckrkpneiik nkqcgydvrk psyilgicfa qnlradifaq kakwh
//
LOCUS       XP_076545757             221 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076545757
VERSION     XP_076545757.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689642.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="tetraspanin 26A isoform X6"
                     /calculated_mol_wt=24929
     Region          18..191
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          115..221
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cl02781"
                     /db_xref="CDD:351888"
     Site            order(116,121,125,127..128,131,145,148..149,153..154)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239401"
     CDS             1..221
                     /gene="Tsp26A"
                     /coded_by="XM_076689642.1:465..1130"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mpavrkyrrd tsevscclky vifgfnvmfw wlglgimavg vwawtekdtf nnlsrltnva
       61 ldpafililv gtvtfiigft gcvgalrent cllaayaifl allllmeiav gvlgfvfkdw
      121 iksqatggfq afiihyredp dqqnlidwiq edwlqccgie gpkdwdrnny fncsssdigs
      181 reacgvpfsc ckrkpneiik nkqcgydvrk psyknikkkg c
//
LOCUS       XP_034184826             311 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 26A isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034184826
VERSION     XP_034184826.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328935.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="tetraspanin 26A isoform X7"
                     /calculated_mol_wt=34810
     Region          18..191
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          115..236
                     /region_name="TM4SF9_like_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL), TM4SF9_like subfamily.
                     Tetraspanins are trans-membrane proteins with 4
                     trans-membrane segments. Both the N- and C-termini lie on
                     the intracellular side of the membrane. This alignment...;
                     cd03159"
                     /db_xref="CDD:239412"
     Site            order(116,121,125,127..128,131,145,148..149,153..154)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239412"
     CDS             1..311
                     /gene="Tsp26A"
                     /coded_by="XM_034328935.2:467..1402"
                     /db_xref="GeneID:117606474"
ORIGIN      
        1 mpavrkyrrd tsevscclky vifgfnvmfw wlglgimavg vwawtekdtf nnlsrltnva
       61 ldpafililv gtvtfiigft gcvgalrent cllaayaifl allllmeiav gvlgfvfkdw
      121 iksqatggfq afiihyredp dqqnlidwiq edwlqccgie gpkdwdrnny fncsssdigs
      181 reacgvpfsc ckrkpneiik nkqcgydvrk psytgersiy ergclragee wlelnlvpva
      241 gtvvstmvlq nfevakviye kgciqageew vqrnllsiat gavgtafaqi lgicfaqnlr
      301 adifaqkakw h
//
LOCUS       XP_034185372             261 aa            linear   INV 03-SEP-2025
DEFINITION  yip1 domain-containing 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185372
VERSION     XP_034185372.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329481.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..261
                     /product="yip1 domain-containing 1"
                     /calculated_mol_wt=28351
     Region          <94..261
                     /region_name="YIP1"
                     /note="Rab GTPase interacting factor, Golgi membrane
                     protein [Intracellular trafficking and secretion];
                     cl46908"
                     /db_xref="CDD:481248"
     CDS             1..261
                     /gene="Yip1d1"
                     /coded_by="XM_034329481.2:137..922"
                     /db_xref="GeneID:117606693"
ORIGIN      
        1 msdyrnqqny wgqaapppny nfdssgfgqp nqqfefqtyn dqqsvnyssy tqksfldpte
       61 svytgdmfgq dnfpkgpagf gdteeeppll eelginpdri lrktlavlnp fhrsgqidda
      121 syllqdsdla gpvancllla aflllagska hfgyvyglam tscilmyill slmtstsnit
      181 lssvasvlgy cllpvvalag fsvfsslqga aglvlavltv twatlsasrl fctmsgeknq
      241 rlliaypcvl lygvftlivi f
//
LOCUS       XP_034185374             170 aa            linear   INV 03-SEP-2025
DEFINITION  small ribonucleoprotein particle U1 subunit C [Osmia lignaria
            lignaria].
ACCESSION   XP_034185374
VERSION     XP_034185374.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329483.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..170
                     /product="small ribonucleoprotein particle U1 subunit C"
                     /calculated_mol_wt=18004
     Region          1..38
                     /region_name="zf-U1"
                     /note="U1 zinc finger; pfam06220"
                     /db_xref="CDD:368798"
     CDS             1..170
                     /gene="snRNP-U1-C"
                     /coded_by="XM_034329483.2:165..677"
                     /db_xref="GeneID:117606695"
ORIGIN      
        1 mpkyycdycd tylthdspsv rkthcqgrkh kdnvkyfyqk wmeeqaqhli dattaafkag
       61 kiasnpfaan kgaaippppn lgprpgvppq gppgmmpppg mhpggpmgpg gpmmmgphgp
      121 mppmmgmrpp mmgpmgpmgp mmgpmgpmgp mrppmngppp pmggpppmkk
//
LOCUS       XP_034185375             166 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein S24 [Osmia lignaria lignaria].
ACCESSION   XP_034185375
VERSION     XP_034185375.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329484.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="mitochondrial ribosomal protein S24"
                     /calculated_mol_wt=19262
     Region          32..166
                     /region_name="MRP-S24"
                     /note="Mitochondrial ribosome subunit S24; pfam14955"
                     /db_xref="CDD:464401"
     CDS             1..166
                     /gene="mRpS24"
                     /coded_by="XM_034329484.2:143..643"
                     /db_xref="GeneID:117606696"
ORIGIN      
        1 mslvsyirpv nillnqnvit rrclhasavl nktqaghyrp ktrvkqplty emanppyqia
       61 arkswnswnt ssikdgnris etaiedmfir sfmqgtwhgf flseiiikrq hnviriagii
      121 irrltpikiy fligyteell sywlhcpvkl elvstenkdd viykii
//
LOCUS       XP_034190219             353 aa            linear   INV 03-SEP-2025
DEFINITION  serine protease inhibitor dipetalogastin-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034190219
VERSION     XP_034190219.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334328.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190219.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="serine protease inhibitor dipetalogastin-like"
                     /calculated_mol_wt=39245
     Region          44..78
                     /region_name="KAZAL_FS"
                     /note="Kazal type serine protease inhibitors and
                     follistatin-like domains. Kazal inhibitors inhibit serine
                     proteases, such as, trypsin, chyomotrypsin, avian
                     ovomucoids, and elastases. The inhibitory domain has one
                     reactive site peptide bond, which serves the...; cd00104"
                     /db_xref="CDD:238052"
     Region          91..127
                     /region_name="KAZAL_FS"
                     /note="Kazal type serine protease inhibitors and
                     follistatin-like domains. Kazal inhibitors inhibit serine
                     proteases, such as, trypsin, chyomotrypsin, avian
                     ovomucoids, and elastases. The inhibitory domain has one
                     reactive site peptide bond, which serves the...; cl00097"
                     /db_xref="CDD:412159"
     Region          144..178
                     /region_name="KAZAL_FS"
                     /note="Kazal type serine protease inhibitors and
                     follistatin-like domains. Kazal inhibitors inhibit serine
                     proteases, such as, trypsin, chyomotrypsin, avian
                     ovomucoids, and elastases. The inhibitory domain has one
                     reactive site peptide bond, which serves the...; cd00104"
                     /db_xref="CDD:238052"
     Region          <202..236
                     /region_name="KAZAL_FS"
                     /note="Kazal type serine protease inhibitors and
                     follistatin-like domains. Kazal inhibitors inhibit serine
                     proteases, such as, trypsin, chyomotrypsin, avian
                     ovomucoids, and elastases. The inhibitory domain has one
                     reactive site peptide bond, which serves the...; cl00097"
                     /db_xref="CDD:412159"
     Region          <265..299
                     /region_name="KAZAL"
                     /note="Kazal type serine protease inhibitors; smart00280"
                     /db_xref="CDD:197624"
     CDS             1..353
                     /gene="LOC117608756"
                     /coded_by="XM_034334328.2:5..1066"
                     /db_xref="GeneID:117608756"
ORIGIN      
        1 mryilvllgi vcsfkdgwtl swsdkvifyq dceifknpdl yggpicgtds etyanslyld
       61 cmnyqtfqnv gtlhsgsclp mdeyckanlf ykpvcgsdgn tytnlesllc vnykrsqnvt
      121 vtskeacenl dhcyrhgitf ygtnpvcgsn gftyqnaaql kclqrrntdl kilydggctt
      181 rdvynvyghs ekvceiaktr yewnpvcasd gitysnpfef lcyeadmnql vsdgecgtst
      241 qnscaqlela dtasknssea ftpedevcgs dgvtyqsthh lqcyssrnky lsvkhsgpcs
      301 gpddspcgni peedlrlpvc gsdsfsyvsp ealwcaklrf pvkqlmfvhd gpc
//
LOCUS       XP_034190359             243 aa            linear   INV 03-SEP-2025
DEFINITION  48 related 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190359
VERSION     XP_034190359.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334468.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="48 related 1 isoform X1"
                     /calculated_mol_wt=26902
     Region          112..167
                     /region_name="bHLH_TS_PTF1A"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     pancreas transcription factor 1 subunit alpha (PTF1A) and
                     similar proteins; cd11417"
                     /db_xref="CDD:381423"
     Site            order(113,117,119..121,123,128,146..149)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381423"
     Site            order(126..127,130..131,133..134,150,153,157,159..160,164,
                     166..167)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381423"
     CDS             1..243
                     /gene="Fer1"
                     /coded_by="XM_034334468.2:355..1086"
                     /db_xref="GeneID:117608828"
ORIGIN      
        1 mysmdnleld mmnrqymyea hsflgnpaip alpphcgapt tatlpsipsq ltshpagstg
       61 stsgseiyly denssdnesa yssdqenhar ergqsnrrng tsgksprqav qqrqaanmre
      121 rrrmqninda feglrahipt lpyekrlskv dtlklaigyi kflnelvrad kgndpltgng
      181 glsrcsgrdd skkvivrgse gnpfifhsls wsrksdispn gtmyakvwtp edprtsknes
      241 tfe
//
LOCUS       XP_034190360             242 aa            linear   INV 03-SEP-2025
DEFINITION  48 related 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190360
VERSION     XP_034190360.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334469.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="48 related 1 isoform X2"
                     /calculated_mol_wt=26845
     Region          112..167
                     /region_name="bHLH_TS_PTF1A"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     pancreas transcription factor 1 subunit alpha (PTF1A) and
                     similar proteins; cd11417"
                     /db_xref="CDD:381423"
     Site            order(113,117,119..121,123,128,146..149)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381423"
     Site            order(126..127,130..131,133..134,150,153,157,159..160,164,
                     166..167)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381423"
     CDS             1..242
                     /gene="Fer1"
                     /coded_by="XM_034334469.2:355..1083"
                     /db_xref="GeneID:117608828"
ORIGIN      
        1 mysmdnleld mmnrqymyea hsflgnpaip alpphcgapt tatlpsipsq ltshpagstg
       61 stsgseiyly denssdnesa yssdqenhar ergqsnrrng tsgksprqav qqrqaanmre
      121 rrrmqninda feglrahipt lpyekrlskv dtlklaigyi kflnelvrad kgndpltgng
      181 glsrcsgrdd skkvivrseg npfifhslsw srksdispng tmyakvwtpe dprtsknest
      241 fe
//
LOCUS       XP_034194575             117 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610854 [Osmia lignaria lignaria].
ACCESSION   XP_034194575
VERSION     XP_034194575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..117
                     /product="uncharacterized protein LOC117610854"
                     /calculated_mol_wt=13738
     Region          <1..>32
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..117
                     /gene="LOC117610854"
                     /coded_by="XM_034338684.2:761..1114"
                     /db_xref="GeneID:117610854"
ORIGIN      
        1 mnadnferwi qekvlpnipe ntvivmdnvs yhsvqinkpp tkyatktaml nwliknnipc
       61 ndqmrryelh elieknkpqn kifkideiik ahghttlrlp pytyvrvkph ridfgsn
//
LOCUS       XP_076545758             409 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305563 [Osmia lignaria lignaria].
ACCESSION   XP_076545758
VERSION     XP_076545758.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689643.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="uncharacterized protein LOC143305563"
                     /calculated_mol_wt=45500
     Region          65..>194
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     Region          368..408
                     /region_name="PHD_TCF19_like"
                     /note="PHD finger found in Transcription factor 19
                     (TCF-19), Lysine-specific demethylase KDM5A and KDM5B, and
                     other similar proteins; cd15517"
                     /db_xref="CDD:276992"
     CDS             1..409
                     /gene="LOC143305563"
                     /coded_by="XM_076689643.1:1846..3075"
                     /db_xref="GeneID:143305563"
ORIGIN      
        1 mlqsaaeidl plfflantfe fkfiantifn vdesgfstvq kslqkvvark gkrqvgtits
       61 gergvlttmv cagsaagfyv lpmiifkrkk fnndlkigal pgsivtlsdt gyinsqlfve
      121 wlnhlknhvn cnknkkvlll ldghtthskn ldacifardn givllqlpgh ttrrlqpldv
      181 affgplqkyf tqaqetflrq ykgdkisqtq iskllneayg raatvaiaes gfrasgiwpv
      241 nrhifedhrf apfdslqqqh ssntaippsf ndsgssssdf gvdyvpqkkk sktfkdilhe
      301 isppptpnsq sgpssprvgr iaqkavvitn sdnilelnhi nrkrmnkakp pnvgdkkqkv
      361 qiktasfcii ceetkeeemi qcikcltwvh tkcagvspri kkyfcfnca
//
LOCUS       XP_076545759             127 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610850 [Osmia lignaria lignaria].
ACCESSION   XP_076545759
VERSION     XP_076545759.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689644.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..127
                     /product="uncharacterized protein LOC117610850"
                     /calculated_mol_wt=14689
     CDS             1..127
                     /gene="LOC117610850"
                     /coded_by="XM_076689644.1:53..436"
                     /db_xref="GeneID:117610850"
ORIGIN      
        1 mtefqnided syrevvnyle yvggfnlkea vtlsfketvs dclltsyswl grednnlalc
       61 etrivhaihd avlqnkhfpr psrkdvaehl kaalrtaker vrskrrgprn pqhetvdnfw
      121 taeeqsr
//
LOCUS       XP_076545760             421 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305564 [Osmia lignaria lignaria].
ACCESSION   XP_076545760
VERSION     XP_076545760.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689645.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="uncharacterized protein LOC143305564"
                     /calculated_mol_wt=47875
     CDS             1..421
                     /gene="LOC143305564"
                     /coded_by="XM_076689645.1:369..1634"
                     /db_xref="GeneID:143305564"
ORIGIN      
        1 meeghtfwsa lpredclktk ydvlyrgpvt kltsnnretl ytiettdisf alaakgdikt
       61 cnriltkteh pkliiyertr nnilfdndaq pskeilnldi ltytnskfvy vkkhfraqiq
      121 nlyldilser cklerrtiyn slsiasispd efaynlmekp gyiariagev vhiikctpke
      181 vqlfhtdkcy tqlpvtsgnq trfltskthi lmksgtqiec ntiisqyykl nnnwialtpt
      241 pkkaqnpgil kpntniswey keinslassg iysqkdlqel qqhimfplek sallntiarg
      301 mlgeesvqgg linnlftent lshiaentwn kfvekfikfg sissaiimil iiihicklii
      361 dtiirgytlh tlygwsiqil gaifssityl lvlgnsvfpl ydtrlesdgg atasrllrtn
      421 h
//
LOCUS       XP_034190698            1560 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608993 [Osmia lignaria lignaria].
ACCESSION   XP_034190698
VERSION     XP_034190698.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334807.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1560
                     /product="uncharacterized protein LOC117608993"
                     /calculated_mol_wt=175465
     Region          1115..>1276
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     CDS             1..1560
                     /gene="LOC117608993"
                     /coded_by="XM_034334807.2:85..4767"
                     /db_xref="GeneID:117608993"
ORIGIN      
        1 msinvtvngn hlnirrgrmv lsdldqikkn erdrrrrlrl eqvrqqskei sdrllerakn
       61 iakeelkkle tndklnlrqi hnekimevqq kyqedmadig qahisatlep dynaimkekd
      121 rqnklaalkr gkeaikqmkn iqqesvqqqq heerlrqvre venlrstmia klpqktisek
      181 dtvrddaqee eskqhkpakk kvkkfiskks pgkitksyik vvgsdlrths sllksktknd
      241 svveqrskts ldatpsveqt vcitqlennr hsgeevlnas tkknkttryn pedyaqatsn
      301 stnsdsissf sddssyfsdn neqstktrts kyikcpaaak vqlydhskhq sntydkpvgv
      361 vekihtwsep saidlaqtie qaetvethll esrkynaqkr gedaalrekv rrdyqtlmqn
      421 lnqlaseerk lkasqveryp ksthtqehrr ilrdqhkkkl nhavrsllse ecleqcvsqp
      481 merqitlppr vsngnvdvhg tweepccskr adakkisrkk ceeeeesree qildmlkkve
      541 rqkrlllqef gvdlpndifn asmkplfeke ksvqtesqen anvpkpsspe irvinvpqdk
      601 ndkkgksdsp iktaeiavqt ttgdkrsive dkgtqveltq edkdkaksdt etvtkhyple
      661 pkitvithet dnessssvtt navidadkps skatpkktkr svktskrvps ktfqkssstr
      721 iskaspslkk cpksrypkka ntedessnri kmyvdengln ikvqpppvte vavdvstqss
      781 qlyspsiqeq stskphwiqs rsrikirdfs dtstsfaspp pikprdilea ltnnisilel
      841 ldssvnesmk rlkgdispvs tpetpsprtm rlpsniphpe kitkllksrg lqtnesnsls
      901 svrnscstst dlsdelpsnq kpaalpkfpv slefclcnnp ncnqmhakfd eirsyalknc
      961 pqilqryedl qttcaeriis ltnliekvrn eqkgmefsii taddtslmql pspkfltndl
     1021 qsvrklveni eavhnqlakt liesqkiiks kavkdegnqt kekqivivek sntpkkvtdp
     1081 veskgvlkdk vkpkiirdek vnirldrfql qaepgtstvk sgfrlvskhn eeemieklsk
     1141 eileqsksfn nnvmvtkevs ssrkhsiets tdvtdskdna smnksndrvt aqystkeqkk
     1201 nkdfipiltd ipkvtkavdt ttytngrskp pvslfsgpyr aeiessghel stiiefdtpd
     1261 tinksqnnvr spsstkrvae tqvikstdia kpgenvhlln lrnqrlekec nssikkfssv
     1321 vgtqkikeve ifsdspktsk stggkdviqe tsnkklqynm anqeslqmee svgtdppank
     1381 hyinnkdkit stssnsfsel sgisqiastp sstilkyass peemetalkk lglgwaittl
     1441 kktreasals sssnsdvtpi ntakrispvk kqfdnnyglp disdvssisi keasksteqa
     1501 vllkgrtstp klqnsnsnsi gsnstntnvs enfqepnngl iipnisltkt ksniktlenv
//
LOCUS       XP_034190702             775 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 reductase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190702
VERSION     XP_034190702.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334811.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190702.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..775
                     /product="cytochrome P450 reductase isoform X1"
                     /calculated_mol_wt=88109
     Region          158..295
                     /region_name="Flavodoxin_1"
                     /note="pfam00258"
                     /db_xref="CDD:425562"
     Region          353..774
                     /region_name="CYPOR"
                     /note="NADPH cytochrome p450 reductase (CYPOR) serves as
                     an electron donor in several oxygenase systems and is a
                     component of nitric oxide synthases and methionine
                     synthase reductases. CYPOR transfers two electrons from
                     NADPH to the heme of cytochrome p450 via...; cd06204"
                     /db_xref="CDD:99801"
     Site            order(375,551,553,608..609,640..641,669..670,675,677,679,
                     729,733,736)
                     /site_type="other"
                     /note="NADP binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(396,531..534,549..551,565..568,774)
                     /site_type="other"
                     /note="FAD binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(531,533..534)
                     /site_type="other"
                     /note="FAD binding motif [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(534,703,772,774)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:99801"
     Site            order(565,568,571)
                     /site_type="other"
                     /note="phosphate binding motif [ion binding]"
                     /db_xref="CDD:99801"
     Site            order(604,608..611,613)
                     /site_type="other"
                     /note="beta-alpha-beta structure motif"
                     /db_xref="CDD:99801"
     CDS             1..775
                     /gene="Cpr"
                     /coded_by="XM_034334811.2:16..2343"
                     /db_xref="GeneID:117608994"
ORIGIN      
        1 myaefhrscr sevgtltiha asvnraprrl ahrrgvnvlv rqlhcdnfdg thflpidiyt
       61 ltagatqpka irmagspvle seeraevlde plfstldivl lsalllaalw wlmrrnkqet
      121 ytpaaksysi qptmfptvqp sensfikklk tsgrslvvfy gsqtgtaeef agrlakegir
      181 yrmkgmvadp eecdmeelih lktipnslav fclatygegd ptdnamefvd wlkngdadln
      241 gltyavfglg nktyehynev alyvdhrleq lgatrvfelg mgdddanied dfitwkdkfw
      301 pavcdffgie gagedvsirq ykltehvdlp aeriytgeia rlhsfknqra pfdaknpyla
      361 pvkvnrelhg ptserscmhi efdidgskmr yetgdhlavy pvnnteqvnk igekcgvdld
      421 tvftltntde estkkhpfpc pcsyrtalth ylditsnprt hvlkelaeyc tdpkdkeklk
      481 lmtstsaegk aayhqwvvqe nrnivhiled iptlkpaldh lceilprlqc ryysissspk
      541 lhptsihita vvveyktptg rinrgvttsw lkekhpsdpp cyvpifvrks qfrlptrtst
      601 piilvgpgtg iapfrgfiqe rdlakkegke vgdtilyfgc rkreedflyr eeleeyvkng
      661 slilhtafsr dqpekvyvth llennkdelw rvigkqnghi yvcglvyklm vliithtfvy
      721 ihlfifhsda knmardvhni llkvvmekgn maeldaadyi kkmdsqkrys sdvws
//
LOCUS       XP_076545761             751 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 reductase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545761
VERSION     XP_076545761.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689646.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..751
                     /product="cytochrome P450 reductase isoform X2"
                     /calculated_mol_wt=85178
     Region          158..295
                     /region_name="Flavodoxin_1"
                     /note="pfam00258"
                     /db_xref="CDD:425562"
     Region          353..750
                     /region_name="CYPOR"
                     /note="NADPH cytochrome p450 reductase (CYPOR) serves as
                     an electron donor in several oxygenase systems and is a
                     component of nitric oxide synthases and methionine
                     synthase reductases. CYPOR transfers two electrons from
                     NADPH to the heme of cytochrome p450 via...; cd06204"
                     /db_xref="CDD:99801"
     Site            order(375,551,553,608..609,640..641,669..670,675,677,679,
                     705,709,712)
                     /site_type="other"
                     /note="NADP binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(396,531..534,549..551,565..568,750)
                     /site_type="other"
                     /note="FAD binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(531,533..534)
                     /site_type="other"
                     /note="FAD binding motif [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(534,703,748,750)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:99801"
     Site            order(565,568,571)
                     /site_type="other"
                     /note="phosphate binding motif [ion binding]"
                     /db_xref="CDD:99801"
     Site            order(604,608..611,613)
                     /site_type="other"
                     /note="beta-alpha-beta structure motif"
                     /db_xref="CDD:99801"
     CDS             1..751
                     /gene="Cpr"
                     /coded_by="XM_076689646.1:16..2271"
                     /db_xref="GeneID:117608994"
ORIGIN      
        1 myaefhrscr sevgtltiha asvnraprrl ahrrgvnvlv rqlhcdnfdg thflpidiyt
       61 ltagatqpka irmagspvle seeraevlde plfstldivl lsalllaalw wlmrrnkqet
      121 ytpaaksysi qptmfptvqp sensfikklk tsgrslvvfy gsqtgtaeef agrlakegir
      181 yrmkgmvadp eecdmeelih lktipnslav fclatygegd ptdnamefvd wlkngdadln
      241 gltyavfglg nktyehynev alyvdhrleq lgatrvfelg mgdddanied dfitwkdkfw
      301 pavcdffgie gagedvsirq ykltehvdlp aeriytgeia rlhsfknqra pfdaknpyla
      361 pvkvnrelhg ptserscmhi efdidgskmr yetgdhlavy pvnnteqvnk igekcgvdld
      421 tvftltntde estkkhpfpc pcsyrtalth ylditsnprt hvlkelaeyc tdpkdkeklk
      481 lmtstsaegk aayhqwvvqe nrnivhiled iptlkpaldh lceilprlqc ryysissspk
      541 lhptsihita vvveyktptg rinrgvttsw lkekhpsdpp cyvpifvrks qfrlptrtst
      601 piilvgpgtg iapfrgfiqe rdlakkegke vgdtilyfgc rkreedflyr eeleeyvkng
      661 slilhtafsr dqpekvyvth llennkdelw rvigkqnghi yvcgdaknma rdvhnillkv
      721 vmekgnmael daadyikkmd sqkryssdvw s
//
LOCUS       XP_034190700             718 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 reductase isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190700
VERSION     XP_034190700.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334809.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..718
                     /product="cytochrome P450 reductase isoform X3"
                     /calculated_mol_wt=81564
     Region          101..238
                     /region_name="Flavodoxin_1"
                     /note="pfam00258"
                     /db_xref="CDD:425562"
     Region          296..717
                     /region_name="CYPOR"
                     /note="NADPH cytochrome p450 reductase (CYPOR) serves as
                     an electron donor in several oxygenase systems and is a
                     component of nitric oxide synthases and methionine
                     synthase reductases. CYPOR transfers two electrons from
                     NADPH to the heme of cytochrome p450 via...; cd06204"
                     /db_xref="CDD:99801"
     Site            order(318,494,496,551..552,583..584,612..613,618,620,622,
                     672,676,679)
                     /site_type="other"
                     /note="NADP binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(339,474..477,492..494,508..511,717)
                     /site_type="other"
                     /note="FAD binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(474,476..477)
                     /site_type="other"
                     /note="FAD binding motif [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(477,646,715,717)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:99801"
     Site            order(508,511,514)
                     /site_type="other"
                     /note="phosphate binding motif [ion binding]"
                     /db_xref="CDD:99801"
     Site            order(547,551..554,556)
                     /site_type="other"
                     /note="beta-alpha-beta structure motif"
                     /db_xref="CDD:99801"
     CDS             1..718
                     /gene="Cpr"
                     /coded_by="XM_034334809.2:164..2320"
                     /db_xref="GeneID:117608994"
ORIGIN      
        1 melptagatq pkairmagsp vleseeraev ldeplfstld ivllsallla alwwlmrrnk
       61 qetytpaaks ysiqptmfpt vqpsensfik klktsgrslv vfygsqtgta eefagrlake
      121 giryrmkgmv adpeecdmee lihlktipns lavfclatyg egdptdname fvdwlkngda
      181 dlngltyavf glgnktyehy nevalyvdhr leqlgatrvf elgmgdddan ieddfitwkd
      241 kfwpavcdff giegagedvs irqykltehv dlpaeriytg eiarlhsfkn qrapfdaknp
      301 ylapvkvnre lhgptsersc mhiefdidgs kmryetgdhl avypvnnteq vnkigekcgv
      361 dldtvftltn tdeestkkhp fpcpcsyrta lthylditsn prthvlkela eyctdpkdke
      421 klklmtstsa egkaayhqwv vqenrnivhi lediptlkpa ldhlceilpr lqcryysiss
      481 spklhptsih itavvveykt ptgrinrgvt tswlkekhps dppcyvpifv rksqfrlptr
      541 tstpiilvgp gtgiapfrgf iqerdlakke gkevgdtily fgcrkreedf lyreeleeyv
      601 kngslilhta fsrdqpekvy vthllennkd elwrvigkqn ghiyvcglvy klmvliitht
      661 fvyihlfifh sdaknmardv hnillkvvme kgnmaeldaa dyikkmdsqk ryssdvws
//
LOCUS       XP_034190703             694 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 reductase isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034190703
VERSION     XP_034190703.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334812.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..694
                     /product="cytochrome P450 reductase isoform X4"
                     /calculated_mol_wt=78634
     Region          101..238
                     /region_name="Flavodoxin_1"
                     /note="pfam00258"
                     /db_xref="CDD:425562"
     Region          296..693
                     /region_name="CYPOR"
                     /note="NADPH cytochrome p450 reductase (CYPOR) serves as
                     an electron donor in several oxygenase systems and is a
                     component of nitric oxide synthases and methionine
                     synthase reductases. CYPOR transfers two electrons from
                     NADPH to the heme of cytochrome p450 via...; cd06204"
                     /db_xref="CDD:99801"
     Site            order(318,494,496,551..552,583..584,612..613,618,620,622,
                     648,652,655)
                     /site_type="other"
                     /note="NADP binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(339,474..477,492..494,508..511,693)
                     /site_type="other"
                     /note="FAD binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(474,476..477)
                     /site_type="other"
                     /note="FAD binding motif [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(477,646,691,693)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:99801"
     Site            order(508,511,514)
                     /site_type="other"
                     /note="phosphate binding motif [ion binding]"
                     /db_xref="CDD:99801"
     Site            order(547,551..554,556)
                     /site_type="other"
                     /note="beta-alpha-beta structure motif"
                     /db_xref="CDD:99801"
     CDS             1..694
                     /gene="Cpr"
                     /coded_by="XM_034334812.2:160..2244"
                     /db_xref="GeneID:117608994"
ORIGIN      
        1 melptagatq pkairmagsp vleseeraev ldeplfstld ivllsallla alwwlmrrnk
       61 qetytpaaks ysiqptmfpt vqpsensfik klktsgrslv vfygsqtgta eefagrlake
      121 giryrmkgmv adpeecdmee lihlktipns lavfclatyg egdptdname fvdwlkngda
      181 dlngltyavf glgnktyehy nevalyvdhr leqlgatrvf elgmgdddan ieddfitwkd
      241 kfwpavcdff giegagedvs irqykltehv dlpaeriytg eiarlhsfkn qrapfdaknp
      301 ylapvkvnre lhgptsersc mhiefdidgs kmryetgdhl avypvnnteq vnkigekcgv
      361 dldtvftltn tdeestkkhp fpcpcsyrta lthylditsn prthvlkela eyctdpkdke
      421 klklmtstsa egkaayhqwv vqenrnivhi lediptlkpa ldhlceilpr lqcryysiss
      481 spklhptsih itavvveykt ptgrinrgvt tswlkekhps dppcyvpifv rksqfrlptr
      541 tstpiilvgp gtgiapfrgf iqerdlakke gkevgdtily fgcrkreedf lyreeleeyv
      601 kngslilhta fsrdqpekvy vthllennkd elwrvigkqn ghiyvcgdak nmardvhnil
      661 lkvvmekgnm aeldaadyik kmdsqkryss dvws
//
LOCUS       XP_076545762             541 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 reductase isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076545762
VERSION     XP_076545762.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689647.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..541
                     /product="cytochrome P450 reductase isoform X5"
                     /calculated_mol_wt=62108
     Region          <33..85
                     /region_name="Flavodoxin_1"
                     /note="pfam00258"
                     /db_xref="CDD:425562"
     Region          143..540
                     /region_name="CYPOR"
                     /note="NADPH cytochrome p450 reductase (CYPOR) serves as
                     an electron donor in several oxygenase systems and is a
                     component of nitric oxide synthases and methionine
                     synthase reductases. CYPOR transfers two electrons from
                     NADPH to the heme of cytochrome p450 via...; cd06204"
                     /db_xref="CDD:99801"
     Site            order(165,341,343,398..399,430..431,459..460,465,467,469,
                     495,499,502)
                     /site_type="other"
                     /note="NADP binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(186,321..324,339..341,355..358,540)
                     /site_type="other"
                     /note="FAD binding pocket [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(321,323..324)
                     /site_type="other"
                     /note="FAD binding motif [chemical binding]"
                     /db_xref="CDD:99801"
     Site            order(324,493,538,540)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:99801"
     Site            order(355,358,361)
                     /site_type="other"
                     /note="phosphate binding motif [ion binding]"
                     /db_xref="CDD:99801"
     Site            order(394,398..401,403)
                     /site_type="other"
                     /note="beta-alpha-beta structure motif"
                     /db_xref="CDD:99801"
     CDS             1..541
                     /gene="Cpr"
                     /coded_by="XM_076689647.1:16..1641"
                     /db_xref="GeneID:117608994"
ORIGIN      
        1 mkrfsnilrv ilkcynyesa iqrrfltlsl aprfevfglg nktyehynev alyvdhrleq
       61 lgatrvfelg mgdddanied dfitwkdkfw pavcdffgie gagedvsirq ykltehvdlp
      121 aeriytgeia rlhsfknqra pfdaknpyla pvkvnrelhg ptserscmhi efdidgskmr
      181 yetgdhlavy pvnnteqvnk igekcgvdld tvftltntde estkkhpfpc pcsyrtalth
      241 ylditsnprt hvlkelaeyc tdpkdkeklk lmtstsaegk aayhqwvvqe nrnivhiled
      301 iptlkpaldh lceilprlqc ryysissspk lhptsihita vvveyktptg rinrgvttsw
      361 lkekhpsdpp cyvpifvrks qfrlptrtst piilvgpgtg iapfrgfiqe rdlakkegke
      421 vgdtilyfgc rkreedflyr eeleeyvkng slilhtafsr dqpekvyvth llennkdelw
      481 rvigkqnghi yvcgdaknma rdvhnillkv vmekgnmael daadyikkmd sqkryssdvw
      541 s
//
LOCUS       XP_034190576             439 aa            linear   INV 03-SEP-2025
DEFINITION  amphiphysin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190576
VERSION     XP_034190576.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334685.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..439
                     /product="amphiphysin isoform X1"
                     /calculated_mol_wt=49634
     Region          26..255
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(45,48..49,53,55..56,59..60,62..63,91..92,94..95,
                     98..99,101..102,105,113,116..117,211,214..215,217,222,
                     225..226,228..229,232..233,236..237,239..240,243..244,
                     246..247)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          376..439
                     /region_name="SH3_Amphiphysin"
                     /note="Src Homology 3 domain of Amphiphysin and related
                     domains; cd11790"
                     /db_xref="CDD:212724"
     Site            order(387..393,412,414..417,432,434..435)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212724"
     CDS             1..439
                     /gene="Amph"
                     /coded_by="XM_034334685.2:166..1485"
                     /db_xref="GeneID:117608927"
ORIGIN      
        1 maeskgalia ktvqkhagra kekflqnlgk vdrtaddifd ehlqnfmrqq naanrlqkef
       61 nnyircvrgf pvcpsvclsd llwskyavqa asktlmdsln eiyesqwtgh dllyvqaqnl
      121 dmlwqdfthk ladqvlvpln tyqsqfpemr kkidkrgrkl vdydsqrhnf qslqcnprkr
      181 delkvsrgke mleeakrtye qlnselhdel palydsrvlf lvtnlqtlfa aeqvfhtesa
      241 kvyseleaiv dklanesqrg sytlkkntep qspkspnans sliintqpaq nnispavdes
      301 apaarntspt tqlngnansn ansndnslnn qdaspqstva askrveelyd ipvdveyeng
      361 tdfsvgattd nlppgvlyrv katykyrred adelsfdvgd iirvveyddp eeqeqgwlmg
      421 ikegtnekgm fpanftkpl
//
LOCUS       XP_034190577             427 aa            linear   INV 03-SEP-2025
DEFINITION  amphiphysin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190577
VERSION     XP_034190577.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334686.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="amphiphysin isoform X2"
                     /calculated_mol_wt=48277
     Region          26..255
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(45,48..49,53,55..56,59..60,62..63,91..92,94..95,
                     98..99,101..102,105,113,116..117,211,214..215,217,222,
                     225..226,228..229,232..233,236..237,239..240,243..244,
                     246..247)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          364..427
                     /region_name="SH3_Amphiphysin"
                     /note="Src Homology 3 domain of Amphiphysin and related
                     domains; cd11790"
                     /db_xref="CDD:212724"
     Site            order(375..381,400,402..405,420,422..423)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212724"
     CDS             1..427
                     /gene="Amph"
                     /coded_by="XM_034334686.2:165..1448"
                     /db_xref="GeneID:117608927"
ORIGIN      
        1 maeskgalia ktvqkhagra kekflqnlgk vdrtaddifd ehlqnfmrqq naanrlqkef
       61 nnyircvrgf pvcpsvclsd llwskyavqa asktlmdsln eiyesqwtgh dllyvqaqnl
      121 dmlwqdfthk ladqvlvpln tyqsqfpemr kkidkrgrkl vdydsqrhnf qslqcnprkr
      181 delkvsrgke mleeakrtye qlnselhdel palydsrvlf lvtnlqtlfa aeqvfhtesa
      241 kvyseleaiv dklanesqrg sytlkkntep qspkspnans sliintqpaq nnispavdes
      301 apaarntspt tqlngnansn ansndnslnn qdaspqstva askrveelyd ipvgattdnl
      361 ppgvlyrvka tykyrredad elsfdvgdii rvveyddpee qeqgwlmgik egtnekgmfp
      421 anftkpl
//
LOCUS       XP_034190578             421 aa            linear   INV 03-SEP-2025
DEFINITION  amphiphysin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190578
VERSION     XP_034190578.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="amphiphysin isoform X3"
                     /calculated_mol_wt=47637
     Region          26..237
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(45,48..49,53,55..56,59..60,62..63,73..74,76..77,
                     80..81,83..84,87,95,98..99,193,196..197,199,204,207..208,
                     210..211,214..215,218..219,221..222,225..226,228..229)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          358..421
                     /region_name="SH3_Amphiphysin"
                     /note="Src Homology 3 domain of Amphiphysin and related
                     domains; cd11790"
                     /db_xref="CDD:212724"
     Site            order(369..375,394,396..399,414,416..417)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212724"
     CDS             1..421
                     /gene="Amph"
                     /coded_by="XM_034334687.2:164..1429"
                     /db_xref="GeneID:117608927"
ORIGIN      
        1 maeskgalia ktvqkhagra kekflqnlgk vdrtaddifd ehlqnfmrqq naanrlqkef
       61 nnyircvrav qaasktlmds lneiyesqwt ghdllyvqaq nldmlwqdft hkladqvlvp
      121 lntyqsqfpe mrkkidkrgr klvdydsqrh nfqslqcnpr krdelkvsrg kemleeakrt
      181 yeqlnselhd elpalydsrv lflvtnlqtl faaeqvfhte sakvyselea ivdklanesq
      241 rgsytlkknt epqspkspna nssliintqp aqnnispavd esapaarnts pttqlngnan
      301 snansndnsl nnqdaspqst vaaskrveel ydipvdveye ngtdfsvgat tdnlppgvly
      361 rvkatykyrr edadelsfdv gdiirvveyd dpeeqeqgwl mgikegtnek gmfpanftkp
      421 l
//
LOCUS       XP_034190579             409 aa            linear   INV 03-SEP-2025
DEFINITION  amphiphysin isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034190579
VERSION     XP_034190579.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334688.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="amphiphysin isoform X4"
                     /calculated_mol_wt=46281
     Region          26..237
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(45,48..49,53,55..56,59..60,62..63,73..74,76..77,
                     80..81,83..84,87,95,98..99,193,196..197,199,204,207..208,
                     210..211,214..215,218..219,221..222,225..226,228..229)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          346..409
                     /region_name="SH3_Amphiphysin"
                     /note="Src Homology 3 domain of Amphiphysin and related
                     domains; cd11790"
                     /db_xref="CDD:212724"
     Site            order(357..363,382,384..387,402,404..405)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212724"
     CDS             1..409
                     /gene="Amph"
                     /coded_by="XM_034334688.2:162..1391"
                     /db_xref="GeneID:117608927"
ORIGIN      
        1 maeskgalia ktvqkhagra kekflqnlgk vdrtaddifd ehlqnfmrqq naanrlqkef
       61 nnyircvrav qaasktlmds lneiyesqwt ghdllyvqaq nldmlwqdft hkladqvlvp
      121 lntyqsqfpe mrkkidkrgr klvdydsqrh nfqslqcnpr krdelkvsrg kemleeakrt
      181 yeqlnselhd elpalydsrv lflvtnlqtl faaeqvfhte sakvyselea ivdklanesq
      241 rgsytlkknt epqspkspna nssliintqp aqnnispavd esapaarnts pttqlngnan
      301 snansndnsl nnqdaspqst vaaskrveel ydipvgattd nlppgvlyrv katykyrred
      361 adelsfdvgd iirvveyddp eeqeqgwlmg ikegtnekgm fpanftkpl
//
LOCUS       XP_034190580             381 aa            linear   INV 03-SEP-2025
DEFINITION  amphiphysin isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034190580
VERSION     XP_034190580.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334689.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..381
                     /product="amphiphysin isoform X5"
                     /calculated_mol_wt=43621
     Region          26..255
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(45,48..49,53,55..56,59..60,62..63,91..92,94..95,
                     98..99,101..102,105,113,116..117,211,214..215,217,222,
                     225..226,228..229,232..233,236..237,239..240,243..244,
                     246..247)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          318..381
                     /region_name="SH3_Amphiphysin"
                     /note="Src Homology 3 domain of Amphiphysin and related
                     domains; cd11790"
                     /db_xref="CDD:212724"
     Site            order(329..335,354,356..359,374,376..377)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212724"
     CDS             1..381
                     /gene="Amph"
                     /coded_by="XM_034334689.2:166..1311"
                     /db_xref="GeneID:117608927"
ORIGIN      
        1 maeskgalia ktvqkhagra kekflqnlgk vdrtaddifd ehlqnfmrqq naanrlqkef
       61 nnyircvrgf pvcpsvclsd llwskyavqa asktlmdsln eiyesqwtgh dllyvqaqnl
      121 dmlwqdfthk ladqvlvpln tyqsqfpemr kkidkrgrkl vdydsqrhnf qslqcnprkr
      181 delkvsrgke mleeakrtye qlnselhdel palydsrvlf lvtnlqtlfa aeqvfhtesa
      241 kvyseleaiv dklanesqrg sytlkkntep qspkspnans sliintqpaq nnispdveye
      301 ngtdfsvgat tdnlppgvly rvkatykyrr edadelsfdv gdiirvveyd dpeeqeqgwl
      361 mgikegtnek gmfpanftkp l
//
LOCUS       XP_034190581             369 aa            linear   INV 03-SEP-2025
DEFINITION  amphiphysin isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034190581
VERSION     XP_034190581.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334690.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="amphiphysin isoform X6"
                     /calculated_mol_wt=42265
     Region          26..255
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(45,48..49,53,55..56,59..60,62..63,91..92,94..95,
                     98..99,101..102,105,113,116..117,211,214..215,217,222,
                     225..226,228..229,232..233,236..237,239..240,243..244,
                     246..247)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          306..369
                     /region_name="SH3_Amphiphysin"
                     /note="Src Homology 3 domain of Amphiphysin and related
                     domains; cd11790"
                     /db_xref="CDD:212724"
     Site            order(317..323,342,344..347,362,364..365)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212724"
     CDS             1..369
                     /gene="Amph"
                     /coded_by="XM_034334690.2:165..1274"
                     /db_xref="GeneID:117608927"
ORIGIN      
        1 maeskgalia ktvqkhagra kekflqnlgk vdrtaddifd ehlqnfmrqq naanrlqkef
       61 nnyircvrgf pvcpsvclsd llwskyavqa asktlmdsln eiyesqwtgh dllyvqaqnl
      121 dmlwqdfthk ladqvlvpln tyqsqfpemr kkidkrgrkl vdydsqrhnf qslqcnprkr
      181 delkvsrgke mleeakrtye qlnselhdel palydsrvlf lvtnlqtlfa aeqvfhtesa
      241 kvyseleaiv dklanesqrg sytlkkntep qspkspnans sliintqpaq nnispgattd
      301 nlppgvlyrv katykyrred adelsfdvgd iirvveyddp eeqeqgwlmg ikegtnekgm
      361 fpanftkpl
//
LOCUS       XP_076545763             351 aa            linear   INV 03-SEP-2025
DEFINITION  amphiphysin isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076545763
VERSION     XP_076545763.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689648.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..351
                     /product="amphiphysin isoform X7"
                     /calculated_mol_wt=40268
     Region          26..237
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(45,48..49,53,55..56,59..60,62..63,73..74,76..77,
                     80..81,83..84,87,95,98..99,193,196..197,199,204,207..208,
                     210..211,214..215,218..219,221..222,225..226,228..229)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153222"
     Region          288..351
                     /region_name="SH3_Amphiphysin"
                     /note="Src Homology 3 domain of Amphiphysin and related
                     domains; cd11790"
                     /db_xref="CDD:212724"
     Site            order(299..305,324,326..329,344,346..347)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212724"
     CDS             1..351
                     /gene="Amph"
                     /coded_by="XM_076689648.1:30..1085"
                     /db_xref="GeneID:117608927"
ORIGIN      
        1 maeskgalia ktvqkhagra kekflqnlgk vdrtaddifd ehlqnfmrqq naanrlqkef
       61 nnyircvrav qaasktlmds lneiyesqwt ghdllyvqaq nldmlwqdft hkladqvlvp
      121 lntyqsqfpe mrkkidkrgr klvdydsqrh nfqslqcnpr krdelkvsrg kemleeakrt
      181 yeqlnselhd elpalydsrv lflvtnlqtl faaeqvfhte sakvyselea ivdklanesq
      241 rgsytlkknt epqspkspna nssliintqp aqnnispgat tdnlppgvly rvkatykyrr
      301 edadelsfdv gdiirvveyd dpeeqeqgwl mgikegtnek gmfpanftkp l
//
LOCUS       XP_076545764             438 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608926 [Osmia lignaria lignaria].
ACCESSION   XP_076545764
VERSION     XP_076545764.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689649.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..438
                     /product="uncharacterized protein LOC117608926"
                     /calculated_mol_wt=49816
     Region          32..138
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..438
                     /gene="LOC117608926"
                     /coded_by="XM_076689649.1:286..1602"
                     /db_xref="GeneID:117608926"
ORIGIN      
        1 mvestdicvl qwagharyit ekfsellarq alvdvtlice eqklcvhklv lasnstyfeh
       61 lkkpclmdil fttceehlfi vkeilqqdlg qepiiflkdl nfeilkamve fmycgettvp
      121 yrllssllta tktfkvkelt nvvnammtsh venfeaniht dkkvieskii rnkyngsdyv
      181 hvkcnelekd kcflvcnesn sdvnlsensp lennavtdpp eeelehmlye esqdfetnad
      241 qsnklfsmtg skleenknst lfvnckskev sngysddrmi nsntlctnda lkpilyeqcc
      301 dlsdiddcee nsmslsqscl qqiekdfaqy kfeatdvpsk igkcvkvyth krrksideik
      361 tkftdvnnii qspsstdcid lldepstndi pvtlltsldm esaeyiisln teniqsivga
      421 tlteqhtlnt gnrrdyeq
//
LOCUS       XP_034190704             272 aa            linear   INV 03-SEP-2025
DEFINITION  ras-related protein Rab-34 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190704
VERSION     XP_034190704.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334813.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="ras-related protein Rab-34 isoform X1"
                     /calculated_mol_wt=31209
     Region          58..223
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            64..71
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(66..72,115,170..171,173,202..204)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            89
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            93..95
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            112..115
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(114..115,131..132)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            170..173
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            202..204
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..272
                     /gene="LOC117608995"
                     /coded_by="XM_034334813.2:141..959"
                     /db_xref="GeneID:117608995"
ORIGIN      
        1 mletrsavyk mievareerq innwpppfss eitlyqernf htvvrrscmr kcltlkiska
       61 ivigdvsvgk sclinrfchk ifdnnykati gvdfeverfd ilgipfhlqi wdtagqerfk
      121 siaasyyrga nvimivfdla nlvslghcqq wfneaaqcnt epyyvfligt krdllsnqvy
      181 eiiekraaem arklnaefwa vsartgdgvs elfaraavls fqsmvlnece smkpesinig
      241 sdlitlnpdk ertkrksrrn rcfdctnlvi ey
//
LOCUS       XP_034190705             253 aa            linear   INV 03-SEP-2025
DEFINITION  ras-related protein Rab-36 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190705
VERSION     XP_034190705.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334814.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="ras-related protein Rab-36 isoform X2"
                     /calculated_mol_wt=28918
     Region          39..204
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            45..52
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(47..53,96,151..152,154,183..185)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            70
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            74..76
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            93..96
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(95..96,112..113)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            151..154
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            183..185
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..253
                     /gene="LOC117608995"
                     /coded_by="XM_034334814.2:90..851"
                     /db_xref="GeneID:117608995"
ORIGIN      
        1 minnwpppfs seitlyqern fhtvvrrscm rkcltlkisk aivigdvsvg ksclinrfch
       61 kifdnnykat igvdfeverf dilgipfhlq iwdtagqerf ksiaasyyrg anvimivfdl
      121 anlvslghcq qwfneaaqcn tepyyvflig tkrdllsnqv yeiiekraae marklnaefw
      181 avsartgdgv selfaraavl sfqsmvlnec esmkpesini gsdlitlnpd kertkrksrr
      241 nrcfdctnlv iey
//
LOCUS       XP_076545765             263 aa            linear   INV 03-SEP-2025
DEFINITION  acidic leucine-rich nuclear phosphoprotein 32 mapmodulin isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_076545765
VERSION     XP_076545765.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689650.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="acidic leucine-rich nuclear phosphoprotein 32
                     mapmodulin isoform X1"
                     /calculated_mol_wt=29279
     Region          3..145
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          21..43
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          44..65
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          66..88
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          114..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..263
                     /gene="Mapmodulin"
                     /coded_by="XM_076689650.1:1145..1936"
                     /db_xref="GeneID:117608928"
ORIGIN      
        1 mllislrpsw tsplchqltr ikelvldncr sthivgltde fvaleslsli nvgltslkgf
       61 pklsnlkkle lsdnrisggl nflhsspklt hlnlsgnkik dletlqplke fknlksldlf
      121 nnevtnvdny reklfslips lrcldgfdtd dcevddsege ddevngnedg dgdaneedse
      181 ealetilvsd eeddedfddg gvgletvykd gledesdedd flcdedeedd dddneedeqd
      241 eeeessparg kkrkhedggd sgn
//
LOCUS       XP_034190582             263 aa            linear   INV 03-SEP-2025
DEFINITION  acidic leucine-rich nuclear phosphoprotein 32 mapmodulin isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190582
VERSION     XP_034190582.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334691.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="acidic leucine-rich nuclear phosphoprotein 32
                     mapmodulin isoform X1"
                     /calculated_mol_wt=29279
     Region          3..145
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          21..43
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          44..65
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          66..88
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          114..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..263
                     /gene="Mapmodulin"
                     /coded_by="XM_034334691.2:1059..1850"
                     /db_xref="GeneID:117608928"
ORIGIN      
        1 mllislrpsw tsplchqltr ikelvldncr sthivgltde fvaleslsli nvgltslkgf
       61 pklsnlkkle lsdnrisggl nflhsspklt hlnlsgnkik dletlqplke fknlksldlf
      121 nnevtnvdny reklfslips lrcldgfdtd dcevddsege ddevngnedg dgdaneedse
      181 ealetilvsd eeddedfddg gvgletvykd gledesdedd flcdedeedd dddneedeqd
      241 eeeessparg kkrkhedggd sgn
//
LOCUS       XP_034190583             261 aa            linear   INV 03-SEP-2025
DEFINITION  acidic leucine-rich nuclear phosphoprotein 32 mapmodulin isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190583
VERSION     XP_034190583.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334692.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..261
                     /product="acidic leucine-rich nuclear phosphoprotein 32
                     mapmodulin isoform X2"
                     /calculated_mol_wt=29094
     Region          3..145
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          21..43
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          44..65
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          66..88
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          114..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..261
                     /gene="Mapmodulin"
                     /coded_by="XM_034334692.2:1145..1930"
                     /db_xref="GeneID:117608928"
ORIGIN      
        1 mllislrpsw tsplchqltr ikelvldncr sthivgltde fvaleslsli nvgltslkgf
       61 pklsnlkkle lsdnrisggl nflhsspklt hlnlsgnkik dletlqplke fknlksldlf
      121 nnevtnvdny reklfslips lrcldgfdtd dcevddsege ddevngnedg dgdaneedse
      181 eetilvsdee ddedfddggv gletvykdgl edesdeddfl cdedeedddd dneedeqdee
      241 eesspargkk rkhedggdsg n
//
LOCUS       XP_034190584             259 aa            linear   INV 03-SEP-2025
DEFINITION  acidic leucine-rich nuclear phosphoprotein 32 mapmodulin isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034190584
VERSION     XP_034190584.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334693.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..259
                     /product="acidic leucine-rich nuclear phosphoprotein 32
                     mapmodulin isoform X3"
                     /calculated_mol_wt=28852
     Region          17..39
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          40..61
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          <53..141
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          85..109
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          110..123
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..259
                     /gene="Mapmodulin"
                     /coded_by="XM_034334693.2:397..1176"
                     /db_xref="GeneID:117608928"
ORIGIN      
        1 mekrillekr grnpgeikel vldncrsthi vgltdefval eslslinvgl tslkgfpkls
       61 nlkklelsdn risgglnflh sspklthlnl sgnkikdlet lqplkefknl ksldlfnnev
      121 tnvdnyrekl fslipslrcl dgfdtddcev ddsegeddev ngnedgdgda needseeale
      181 tilvsdeedd edfddggvgl etvykdgled esdeddflcd edeedddddn eedeqdeeee
      241 sspargkkrk hedggdsgn
//
LOCUS       XP_034190585             257 aa            linear   INV 03-SEP-2025
DEFINITION  acidic leucine-rich nuclear phosphoprotein 32 mapmodulin isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034190585
VERSION     XP_034190585.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334694.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..257
                     /product="acidic leucine-rich nuclear phosphoprotein 32
                     mapmodulin isoform X4"
                     /calculated_mol_wt=28638
     Region          3..145
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          21..43
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          44..65
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          66..88
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          114..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..257
                     /gene="Mapmodulin"
                     /coded_by="XM_034334694.2:1140..1913"
                     /db_xref="GeneID:117608928"
ORIGIN      
        1 mllislrpsw tsplchqltr ikelvldncr sthivgltde fvaleslsli nvgltslkgf
       61 pklsnlkkle lsdnrisggl nflhsspklt hlnlsgnkik dletlqplke fknlksldlf
      121 nnevtnvdny reklfslips lrcldgfdtd dcevddsege ddevngnedg dgdaneedse
      181 evsdeedded fddggvglet vykdgledes deddflcded eedddddnee deqdeeeess
      241 pargkkrkhe dggdsgn
//
LOCUS       XP_076545766             257 aa            linear   INV 03-SEP-2025
DEFINITION  acidic leucine-rich nuclear phosphoprotein 32 mapmodulin isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_076545766
VERSION     XP_076545766.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689651.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..257
                     /product="acidic leucine-rich nuclear phosphoprotein 32
                     mapmodulin isoform X5"
                     /calculated_mol_wt=28668
     Region          17..39
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          40..61
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          <53..141
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          85..109
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          110..123
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..257
                     /gene="Mapmodulin"
                     /coded_by="XM_076689651.1:397..1170"
                     /db_xref="GeneID:117608928"
ORIGIN      
        1 mekrillekr grnpgeikel vldncrsthi vgltdefval eslslinvgl tslkgfpkls
       61 nlkklelsdn risgglnflh sspklthlnl sgnkikdlet lqplkefknl ksldlfnnev
      121 tnvdnyrekl fslipslrcl dgfdtddcev ddsegeddev ngnedgdgda needseeeti
      181 lvsdeedded fddggvglet vykdgledes deddflcded eedddddnee deqdeeeess
      241 pargkkrkhe dggdsgn
//
LOCUS       XP_034190586             253 aa            linear   INV 03-SEP-2025
DEFINITION  acidic leucine-rich nuclear phosphoprotein 32 mapmodulin isoform X6
            [Osmia lignaria lignaria].
ACCESSION   XP_034190586
VERSION     XP_034190586.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334695.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="acidic leucine-rich nuclear phosphoprotein 32
                     mapmodulin isoform X6"
                     /calculated_mol_wt=28211
     Region          17..39
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          40..61
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          <53..141
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          62..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          85..109
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          110..123
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..253
                     /gene="Mapmodulin"
                     /coded_by="XM_034334695.2:389..1150"
                     /db_xref="GeneID:117608928"
ORIGIN      
        1 mekrillekr grnpgeikel vldncrsthi vgltdefval eslslinvgl tslkgfpkls
       61 nlkklelsdn risgglnflh sspklthlnl sgnkikdlet lqplkefknl ksldlfnnev
      121 tnvdnyrekl fslipslrcl dgfdtddcev ddsegeddev ngnedgdgda needseevsd
      181 eeddedfddg gvgletvykd gledesdedd flcdedeedd dddneedeqd eeeessparg
      241 kkrkhedggd sgn
//
LOCUS       XP_076545767             463 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117610829 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545767
VERSION     XP_076545767.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689652.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..463
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117610829"
                     /calculated_mol_wt=53923
     Region          93..164
                     /region_name="HTH_Tnp_Tc5"
                     /note="Tc5 transposase DNA-binding domain; cl12115"
                     /db_xref="CDD:472278"
     CDS             1..463
                     /gene="LOC117610829"
                     /coded_by="XM_076689652.1:1521..2912"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:117610829"
ORIGIN      
        1 maeeveyfss neeeeqkltp ptlekeyvps cknissldrv pfdiklkivm tvnkhptwsf
       61 yglqtrfkrh lrhktdtarf rkdiikggty mdkmeiikkn vydqfietrr reqlvtrrql
      121 qqwamdaamq frdavnedgs nfqfttsasw itnflkvyri snrrvvryvs nkeitspeei
      181 mksaiqfqnl iqsipndyds dyiintdqtg ceyrinvart ythtgqktve lfigdlnkit
      241 hsytaqyslt ksgkllpkvf vclqesgdaf gvrvkaevne ilklcknviv vcsksgklss
      301 dlykkylekv ikpyvgnnpf lmivdswggg ggggghknih myneifvnse nrptcnlqii
      361 ppkctpicqp cdvyfyrqvk ilnkriqnys qhimadrekk elntrydais iqslihylls
      421 aplfkkmiqf awyasklene rdifynvnql cfppdihnkk mym
//
LOCUS       XP_034185373             192 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606694 [Osmia lignaria lignaria].
ACCESSION   XP_034185373
VERSION     XP_034185373.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329482.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..192
                     /product="uncharacterized protein LOC117606694"
                     /calculated_mol_wt=21723
     Region          96..158
                     /region_name="zf-LITAF-like"
                     /note="LITAF-like zinc ribbon domain; pfam10601"
                     /db_xref="CDD:463165"
     CDS             1..192
                     /gene="LOC117606694"
                     /coded_by="XM_034329482.2:121..699"
                     /db_xref="GeneID:117606694"
ORIGIN      
        1 micgtqpyva twcpvcdkrv sdkgrdtygl lahirefhpk hsnqfftqek esrtvskqtt
       61 skgslrtgct nrkseprkvy atrvdtwksh nekklcprck keavptlhar gdklttshig
      121 alcllgcwpl cfvplmmkra kkvrmicplc gymygsyqyk ntglapcntd sedsqarlss
      181 pprsfrlkvt ke
//
LOCUS       XP_076545768             740 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305566 [Osmia lignaria lignaria].
ACCESSION   XP_076545768
VERSION     XP_076545768.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689653.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..740
                     /product="uncharacterized protein LOC143305566"
                     /calculated_mol_wt=84538
     Region          496..683
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(552,586,596,598)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..740
                     /gene="LOC143305566"
                     /coded_by="XM_076689653.1:66..2288"
                     /db_xref="GeneID:143305566"
ORIGIN      
        1 mcnhvecvsc lqnsdntmdi nrsavsgtmm tgggynqlee ilsgmnvscm skriyekcqd
       61 eiidafelaa qreiedagkl ekelaiargd mlpgcnipci pvvadgswmk rsyrggdyns
      121 lsgvgaivgy htkkvlyinv knkfciicqt aakkkeearq hrcfknwgrn qnstgmesaa
      181 ivdgfkcsie mhgliysilv adgdssvykk ildndpykey mvhvrkiect nhllrnfsrk
      241 ineiatkgrf kelraivken alrlrtgvkk aaeyrfkekv tladqiknlk tdmknvashv
      301 fgehqecqrl ayfcnkysdp nrvnyvpqlk ecgiyqsiee amqplfpqae sllyglnnna
      361 vesfnniiak figgerinfg rkgsyqgrvs aavlhfnsre sfsrlstamn keptaivqnt
      421 eqrrkhalqm aakykqnaka cssryekqsc dkdygptaek pdmeeavfss ecerhmltll
      481 qwqtmrhkie aetrdqakse kwiayrkkll tashfgkvcr lrkntprgnt iksimypqiq
      541 ylrsleygre neanalrqle temgihilpc glfidavvpy lgatpdslvd gdtivevkcp
      601 esakdlspae aieqlpairr ifrgdemnki hhyyyqvqgq lhitnrqcgl fclwtkkgik
      661 itkvqrddrf watemeqklk rfyedsmlpe iidsrfnrqq pirepayikq snntctstqe
      721 stcddvhknq nctdectqfi
//
LOCUS       XP_076545769             135 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610951 [Osmia lignaria lignaria].
ACCESSION   XP_076545769
VERSION     XP_076545769.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689654.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..135
                     /product="uncharacterized protein LOC117610951"
                     /calculated_mol_wt=15366
     CDS             1..135
                     /gene="LOC117610951"
                     /coded_by="XM_076689654.1:1347..1754"
                     /db_xref="GeneID:117610951"
ORIGIN      
        1 miatceilkv khvpcgahsi qldvnhailk tkpsslttsr dtdvitlfes desenlsshn
       61 evsvyiilkk ckrittffhs spkksaelkk elnlrqmevt tlvqsvytrw nssyemlrhi
      121 hktldcnnii llikk
//
LOCUS       XP_076545770             135 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610951 [Osmia lignaria lignaria].
ACCESSION   XP_076545770
VERSION     XP_076545770.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689655.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..135
                     /product="uncharacterized protein LOC117610951"
                     /calculated_mol_wt=15366
     CDS             1..135
                     /gene="LOC117610951"
                     /coded_by="XM_076689655.1:1290..1697"
                     /db_xref="GeneID:117610951"
ORIGIN      
        1 miatceilkv khvpcgahsi qldvnhailk tkpsslttsr dtdvitlfes desenlsshn
       61 evsvyiilkk ckrittffhs spkksaelkk elnlrqmevt tlvqsvytrw nssyemlrhi
      121 hktldcnnii llikk
//
LOCUS       XP_076545771             458 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117611086 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545771
VERSION     XP_076545771.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689656.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..458
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117611086"
                     /calculated_mol_wt=53638
     Region          93..164
                     /region_name="HTH_Tnp_Tc5"
                     /note="Tc5 transposase DNA-binding domain; cl12115"
                     /db_xref="CDD:472278"
     CDS             1..458
                     /gene="LOC117611086"
                     /coded_by="XM_076689656.1:1366..2742"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 3 bases in 2 codons"
                     /db_xref="GeneID:117611086"
ORIGIN      
        1 maeeveyfss neeeeqkltp ptlekeyvps cknissldrv pfdiklkivm tvnkhptwsf
       61 yglqtrfkrh lrhktdtarf rkdiikggty mdkmeiikkn vydqfietrr reqlvtrrql
      121 qqwamdaamq frdavnedgs nfqfttsasw itnflkvyri snrrvvryvs nkeitspeei
      181 mksaiqfqnl iqsipndyds dyiintdqtg ceyrinvart ythtgqktve lfigdlnkit
      241 hsytaqyslt ksgkllpkvf vclqesgdaf gvrvkaevne ilklcknviv vcsksgklss
      301 dlykkylekv ikpyvgnnpf lmivdswggg hknihmynei fvnsenrptc nlqiippkct
      361 picqpcdvyf yrqvkilnkr iqnysqhima drekkelntr ydaisiqsli hyllsaplfk
      421 kmiqfawyas klenerdify nvnqlcfppd ihnkkmym
//
LOCUS       XP_076545772             532 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305567 [Osmia lignaria lignaria].
ACCESSION   XP_076545772
VERSION     XP_076545772.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689657.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..532
                     /product="uncharacterized protein LOC143305567"
                     /calculated_mol_wt=59814
     CDS             1..532
                     /gene="LOC143305567"
                     /coded_by="XM_076689657.1:299..1897"
                     /db_xref="GeneID:143305567"
ORIGIN      
        1 mdkkskrgsn frpdrqrkrk aprnrysmeq dtsstsasaa klksskddei iiennygyci
       61 leffsvftal saliicaeck knikffrtap rglgskiavq cscdgvqyin sspfinkafe
      121 inrrivvvmr llgvgregin ifcsmmdicq glaintyysc lenlqiasra vyehiiskav
      181 aeekelteas epntdpthlt vsgdgtwkkr gfsslfgvtt lvgkyskkvi dtvlkssfcq
      241 gcnlwrhkkn edvaayndwy eehedsctin hrgspgkmev daviemfers vekhhakyak
      301 yigdgdaktf kgiididpyh gdpvvikkec vghvqkrmga rlrkikkdnk giggkgpgkl
      361 tdklidelsl yyglairrnp esvddmrnev watyyhksst denpqhkycp pgesswckwq
      421 kavsegilqd fvhenpplse kvlelikpiy enlssenllt rclgsetqnn nesfnsliwt
      481 fapkhihags dtieianhia vcifnegflp vlkildtmsi kigpeshafa sk
//
LOCUS       XP_076545773              79 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305568 [Osmia lignaria lignaria].
ACCESSION   XP_076545773
VERSION     XP_076545773.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689658.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..79
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..79
                     /product="uncharacterized protein LOC143305568"
                     /calculated_mol_wt=9332
     CDS             1..79
                     /gene="LOC143305568"
                     /coded_by="XM_076689658.1:3566..3805"
                     /db_xref="GeneID:143305568"
ORIGIN      
        1 mevprlegia flaiakyypi impafqltlt eeaalllamc rerqkrrrmy esrwaatcte
       61 vdlfdlrylf geeidpdww
//
LOCUS       XP_076545774             448 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305570 [Osmia lignaria lignaria].
ACCESSION   XP_076545774
VERSION     XP_076545774.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689659.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..448
                     /product="uncharacterized protein LOC143305570"
                     /calculated_mol_wt=51666
     Region          204..391
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(260,294,304,306)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..448
                     /gene="LOC143305570"
                     /coded_by="XM_076689659.1:479..1825"
                     /db_xref="GeneID:143305570"
ORIGIN      
        1 mknvashvfg ehqecqrlay fcnkysdpnr vnyvpqlkec giyqsieeam qplfpqaesl
       61 lyglnnnave sfnniiakfi ggerinfgrk gsyqgrvsaa vlhfnsresf srlstamnke
      121 ptaivqnteq rrkhalqmaa kykqnakacs sryekqscdk dygptaekpd meeavfssec
      181 erhmltllqw qtmrhkieae trdqaksekw iayrkkllta shfgkvcrlr kntprgntik
      241 simypqiqyl rsleygrene analrqlete mgihilpcgl fidavvpylg atpdslvdgd
      301 tivevkcpes akdlspaeai eqlpairrif rgdemnkihh yyyqvqgqlh itnrqcglfc
      361 lwtkkgikit kvqrddrfwa temeqklkrf yedsmlpeii dsrfnrqqpi repayikqsn
      421 ntctstqest cddvhknqnc tdectqfi
//
LOCUS       XP_034185627             873 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606808 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185627
VERSION     XP_034185627.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329736.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..873
                     /product="uncharacterized protein LOC117606808 isoform X1"
                     /calculated_mol_wt=99249
     Region          69..104
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <71..224
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          111..145
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..873
                     /gene="LOC117606808"
                     /coded_by="XM_034329736.2:302..2923"
                     /db_xref="GeneID:117606808"
ORIGIN      
        1 mekvkkrlel lhsnqsqnil rnlmlrdfgf ctlhksgtre lesiaernlv lkdhkelqcd
       61 lpgvprttfm ivfspdgtkm asthgnhnvy ineiatgkni ktlsghprtp wciafhpsss
      121 hilasgclgg qvrvwnlngg ckvwnsenlt cisslafhps akllviathn eihfwdwsqs
      181 tpfavvttnr ekvryvsfdn lggklitaie nisridrlrr hydgnisrpt whsvnlnilr
      241 sirscrlrry yrrcqilnny eirtvrnsnn hemvqccrti pterlrtnss tnnplldtvt
      301 vesynkdfis rnttfnqndn vtfqlhttdd grtnnyenid ernsnsntet nstvlkynin
      361 netinsklld tkeidnilns dsldqlerkg eekrinpeal siqssvrafv stekkdndee
      421 ttelnvnynd tplksissis lnstnetvnn fqvllgtdqk edsntddkkf ysqcqpstsk
      481 gftsltnncn gnfmksvitn gseskenvkl ttqlqrnkll qqlcesllen irqsigssnv
      541 dsmlsvrnns drnstfpata dgvdrvescl pnilnfidkv snnsaprtsq ilnarkkyys
      601 thdetnsngl ftnttnsscs clhhseises rtgiphshyf ecinnsrpri tatrryndnt
      661 dfngptqkfq syrvqawdfs ngempditns eknivvrdch ifndssidis sdgkllaayc
      721 nttigiyslq wetlgermys terykssisv sisptqqhll vgstksigiy klvneesenq
      781 tevpldindi nyrnlcdwkp ysilwplplr ylnisdlnik yltkkgsmvl vrellqndqe
      841 vdrykainci gwapqpgqgl vyatntgqln ily
//
LOCUS       XP_034185628             856 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606808 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185628
VERSION     XP_034185628.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329737.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..856
                     /product="uncharacterized protein LOC117606808 isoform X2"
                     /calculated_mol_wt=97228
     Region          69..104
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <71..224
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          111..145
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..856
                     /gene="LOC117606808"
                     /coded_by="XM_034329737.2:303..2873"
                     /db_xref="GeneID:117606808"
ORIGIN      
        1 mekvkkrlel lhsnqsqnil rnlmlrdfgf ctlhksgtre lesiaernlv lkdhkelqcd
       61 lpgvprttfm ivfspdgtkm asthgnhnvy ineiatgkni ktlsghprtp wciafhpsss
      121 hilasgclgg qvrvwnlngg ckvwnsenlt cisslafhps akllviathn eihfwdwsqs
      181 tpfavvttnr ekvryvsfdn lggklitaie nisridrlrr hydgnisrpt whsvnlnilr
      241 sirscrlrry yrrcqilnny eirtvrnsnn hemvqccrti pterlrtnss tnnplldtvt
      301 vesynkdfis rnttfnqndn vtfqlhttdd grtnnyenid ernsnsntet nstvlkynin
      361 netinsklld tkeidnilns dsldqlerkg eekrinpeal siqssvrafv stekkdndee
      421 ttelnvnynd tplksissis lnstnetvnn fqvllgtdqk edsntddkkf ysqcqpstsk
      481 gftsltnncn gnfmksvitn gseskenvkl ttqlqrnkll qqlcesllen irqsigssnv
      541 dsmlsvrnns drnstfpata dgvdrvescl pnilnfidkv snnsaprtsq ilnarkkyys
      601 thdetnsngl ftnttnsscs clhhseises rtgiphshyf ecinnsrpri tatrryndnt
      661 dfngptqkfq syrvqawdfs ngempditns eknivvrdch ifndssidis sdgkllaayc
      721 nttigiyslq wetlgermys terykssisv sisptqqhll vgstksigiy klvneesenq
      781 tevpldindi nyrnlcdwkp ysilwplpls mvlvrellqn dqevdrykai ncigwapqpg
      841 qglvyatntg qlnily
//
LOCUS       XP_034185629             851 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606808 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185629
VERSION     XP_034185629.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329738.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..851
                     /product="uncharacterized protein LOC117606808 isoform X3"
                     /calculated_mol_wt=96698
     Region          69..104
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <71..224
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          111..145
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..851
                     /gene="LOC117606808"
                     /coded_by="XM_034329738.2:303..2858"
                     /db_xref="GeneID:117606808"
ORIGIN      
        1 mekvkkrlel lhsnqsqnil rnlmlrdfgf ctlhksgtre lesiaernlv lkdhkelqcd
       61 lpgvprttfm ivfspdgtkm asthgnhnvy ineiatgkni ktlsghprtp wciafhpsss
      121 hilasgclgg qvrvwnlngg ckvwnsenlt cisslafhps akllviathn eihfwdwsqs
      181 tpfavvttnr ekvryvsfdn lggklitaie nisridrlrr hydgnisrpt whsvnlnilr
      241 sirscrlrry yrrcqilnny eirtvrnsnn hemvqccrti pterlrtnss tnnplldtvt
      301 vesynkdfis rnttfnqndn vtfqlhttdd grtnnyenid ernsnsntet nstvlkynin
      361 netinsklld tkeidnilns dsldqlerkg eekrinpeal siqssvrafv stekkdndee
      421 ttelnvnynd tplksissis lnstnetvnn fqvllgtdqk edsntddkkf ysqcqpstsk
      481 gftsltnncn gnfmksvitn gseskenvkl ttqlqrnkll qqlcesllen irqsigssnv
      541 dsmlsvrnns drnstfpata dgvdrvescl pnilnfidkv snnsaprtsq ilnarkkyys
      601 thdetnsngl ftnttnsscs clhhseises rtgiphshyf ecinnsrpri tatrryndnt
      661 dfngptqkfq syrvqawdfs ngempditns eknivvrdch ifndssidis sdgkllaayc
      721 nttigiyslq wetlgermys terykssisv sisptqqhll vgstksigiy klvneesenq
      781 tevpldindi nyrnlcdwkp ysilwplplr ellqndqevd rykaincigw apqpgqglvy
      841 atntgqlnil y
//
LOCUS       XP_034185630             629 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606808 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185630
VERSION     XP_034185630.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329739.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="uncharacterized protein LOC117606808 isoform X4"
                     /calculated_mol_wt=70874
     Region          69..104
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <71..224
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          111..145
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..629
                     /gene="LOC117606808"
                     /coded_by="XM_034329739.2:293..2182"
                     /db_xref="GeneID:117606808"
ORIGIN      
        1 mekvkkrlel lhsnqsqnil rnlmlrdfgf ctlhksgtre lesiaernlv lkdhkelqcd
       61 lpgvprttfm ivfspdgtkm asthgnhnvy ineiatgkni ktlsghprtp wciafhpsss
      121 hilasgclgg qvrvwnlngg ckvwnsenlt cisslafhps akllviathn eihfwdwsqs
      181 tpfavvttnr ekvryvsfdn lggklitaie nisridrlrr hydgnissqc qpstskgfts
      241 ltnncngnfm ksvitngses kenvklttql qrnkllqqlc esllenirqs igssnvdsml
      301 svrnnsdrns tfpatadgvd rvesclpnil nfidkvsnns aprtsqilna rkkyysthde
      361 tnsnglftnt tnsscsclhh seisesrtgi phshyfecin nsrpritatr ryndntdfng
      421 ptqkfqsyrv qawdfsngem pditnsekni vvrdchifnd ssidissdgk llaaycntti
      481 giyslqwetl germystery kssisvsisp tqqhllvgst ksigiyklvn eesenqtevp
      541 ldindinyrn lcdwkpysil wplplrylni sdlnikyltk kgsmvlvrel lqndqevdry
      601 kaincigwap qpgqglvyat ntgqlnily
//
LOCUS       XP_034185685             725 aa            linear   INV 03-SEP-2025
DEFINITION  macoilin-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185685
VERSION     XP_034185685.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329794.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="macoilin-1 isoform X1"
                     /calculated_mol_wt=83324
     Region          2..663
                     /region_name="Macoilin"
                     /note="Macoilin family; pfam09726"
                     /db_xref="CDD:462859"
     CDS             1..725
                     /gene="LOC117606827"
                     /coded_by="XM_034329794.2:141..2318"
                     /db_xref="GeneID:117606827"
ORIGIN      
        1 mkrrnvecgk lrrplkrnkl tegiygstll ylkflllwam viladfilef rfeflwpfwl
       61 llrsvydsfk yqglafsvff icialtsdmi cfffipvhwl ffaastyvwv qyvwhtdkgv
      121 clptvmlwll flyieaavrl rdlrhmpfhl dlcrpfaahc igypvvtlgf gfksyigyrm
      181 rqrkqkdvak enefylqllq qalpleqqts ttmqpiqqvq sqthittsle qhvktqsnkl
      241 nsqcnpspek srgrnnnnsv etgvqngnlh igsqitpqnq nvttknnhrk sldkgekqee
      301 qhnkhnvtnv sqsekndrrf ihtngntiaq sdiqfierig svndfdanev ekdkfiksgs
      361 cghatmksqt ngsvagkwnn ikenrepsst vntqrerktr qmkstvdnit eqqkqqddyc
      421 qrllmcrrle adikrlksdl qssrqleqel rsqintlqng erqakgdiqq lqhdndqlqs
      481 klhglvtarq ldkqtmssle kriaeerkqr taceaslvse rrarraaeea rsaipppppp
      541 lirqectdtc ktrrsqmeqd lknlrrelkt kdercivlek dvtrckenhk eseillgaln
      601 vlqdktahle dslsaetrik ldlfsalgea krqleiresl irsqekeiem lktkiaqdla
      661 vmpqdtfgpa ptcatsklrl snevrvtgtk irsnespcpg ctvsnldpna taytpknsli
      721 astea
//
LOCUS       XP_034185686             724 aa            linear   INV 03-SEP-2025
DEFINITION  macoilin-1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185686
VERSION     XP_034185686.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329795.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..724
                     /product="macoilin-1 isoform X2"
                     /calculated_mol_wt=83168
     Region          2..662
                     /region_name="Macoilin"
                     /note="Macoilin family; pfam09726"
                     /db_xref="CDD:462859"
     CDS             1..724
                     /gene="LOC117606827"
                     /coded_by="XM_034329795.2:141..2315"
                     /db_xref="GeneID:117606827"
ORIGIN      
        1 mkrrnvecgk lrrplkrnkl tegiygstll ylkflllwam viladfilef rfeflwpfwl
       61 llrsvydsfk yqglafsvff icialtsdmi cfffipvhwl ffaastyvwv qyvwhtdkgv
      121 clptvmlwll flyieaavrl rdlrhmpfhl dlcrpfaahc igypvvtlgf gfksyigyrm
      181 rqrkqkdvak enefylqllq qalpleqqts ttmqpiqqvq sqthittsle qhvktqsnkl
      241 nsqcnpspek srgnnnnsve tgvqngnlhi gsqitpqnqn vttknnhrks ldkgekqeeq
      301 hnkhnvtnvs qsekndrrfi htngntiaqs diqfierigs vndfdaneve kdkfiksgsc
      361 ghatmksqtn gsvagkwnni kenrepsstv ntqrerktrq mkstvdnite qqkqqddycq
      421 rllmcrrlea dikrlksdlq ssrqleqelr sqintlqnge rqakgdiqql qhdndqlqsk
      481 lhglvtarql dkqtmsslek riaeerkqrt aceaslvser rarraaeear saippppppl
      541 irqectdtck trrsqmeqdl knlrrelktk dercivlekd vtrckenhke seillgalnv
      601 lqdktahled slsaetrikl dlfsalgeak rqleiresli rsqekeieml ktkiaqdlav
      661 mpqdtfgpap tcatsklrls nevrvtgtki rsnespcpgc tvsnldpnat aytpknslia
      721 stea
//
LOCUS       XP_034185688             719 aa            linear   INV 03-SEP-2025
DEFINITION  macoilin-1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185688
VERSION     XP_034185688.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329797.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..719
                     /product="macoilin-1 isoform X3"
                     /calculated_mol_wt=82551
     Region          2..657
                     /region_name="Macoilin"
                     /note="Macoilin family; pfam09726"
                     /db_xref="CDD:462859"
     CDS             1..719
                     /gene="LOC117606827"
                     /coded_by="XM_034329797.2:143..2302"
                     /db_xref="GeneID:117606827"
ORIGIN      
        1 mkrrnvecgk lrrplkrnkl tegiygstll ylkflllwam viladfilef rfeflwpfwl
       61 llrsvydsfk yqglafsvff icialtsdmi cfffipvhwl ffaastyvwv qyvwhtdkgv
      121 clptvmlwll flyieaavrl rdlrhmpfhl dlcrpfaahc igypvvtlgf gfksyigyrm
      181 rqrkqkdvak enefylqllq qalpleqqts ttmqpiqqvq sqthittsle qhvktqsnkl
      241 nsqcnpspek srgrnnnnsv etgvqngnlh igsqitpqnq nvttknnhrk sldkgekqee
      301 qhnkhnvtnv sqsekndrrf ihtngntiaq sdiqfierig svndfdanev ekdkfiksgs
      361 cghatmksqt ngsvagkwnn ikenrepsst vntqrerktr qmkstvdnit eqqkqqddyc
      421 qrleadikrl ksdlqssrql eqelrsqint lqngerqakg diqqlqhdnd qlqsklhglv
      481 tarqldkqtm sslekriaee rkqrtaceas lvserrarra aeearsaipp pppplirqec
      541 tdtcktrrsq meqdlknlrr elktkderci vlekdvtrck enhkeseill galnvlqdkt
      601 ahledslsae trikldlfsa lgeakrqlei reslirsqek eiemlktkia qdlavmpqdt
      661 fgpaptcats klrlsnevrv tgtkirsnes pcpgctvsnl dpnataytpk nsliastea
//
LOCUS       XP_034185631             514 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-glucose pyrophosphorylase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185631
VERSION     XP_034185631.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329740.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="UDP-glucose pyrophosphorylase isoform X1"
                     /calculated_mol_wt=57998
     Region          62..479
                     /region_name="UDPGP"
                     /note="UTP--glucose-1-phosphate uridylyltransferase;
                     pfam01704"
                     /db_xref="CDD:460300"
     CDS             1..514
                     /gene="UGP"
                     /coded_by="XM_034329740.2:859..2403"
                     /db_xref="GeneID:117606809"
ORIGIN      
        1 mhdltkldgg dggakllmkg rdtqafrert krdalielqr eleklegtat tgevkeevhr
       61 qfegfthlfk rflqeegpsl ewdriqklpd dairdynslp mpegeevral lnklivikln
      121 gglgtsmgch gpksviavrn gltfldltvq qieylnktyn anvplilmds fntdddtqri
      181 irkykgidvd iytfnqscyp rinrdsllpi akhcdiaddi eawyppghgd fyesfrnsgl
      241 lkkflkegrd ycfisnidnl gatvdfkilk llldktetsp leflmevtdk tradvkggtl
      301 ikyedklrll eiaqvpkdha ddfksvktfk ffntnnlwik lsaiervlek nslnmeiivn
      361 nktfsnglni iqletavgaa mksfegsigi nvprsrflpv kktsdlmlvm snlytlrngs
      421 lvmspqrmfp ttpliklgdn hfskvkeflt rfpsipdlle ldhltvsgdv tfgkgvtlkg
      481 tviiianhge ridlpsgtil enkivsgnlr ildh
//
LOCUS       XP_076545775             514 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-glucose pyrophosphorylase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545775
VERSION     XP_076545775.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689660.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="UDP-glucose pyrophosphorylase isoform X1"
                     /calculated_mol_wt=57998
     Region          62..479
                     /region_name="UDPGP"
                     /note="UTP--glucose-1-phosphate uridylyltransferase;
                     pfam01704"
                     /db_xref="CDD:460300"
     CDS             1..514
                     /gene="UGP"
                     /coded_by="XM_076689660.1:326..1870"
                     /db_xref="GeneID:117606809"
ORIGIN      
        1 mhdltkldgg dggakllmkg rdtqafrert krdalielqr eleklegtat tgevkeevhr
       61 qfegfthlfk rflqeegpsl ewdriqklpd dairdynslp mpegeevral lnklivikln
      121 gglgtsmgch gpksviavrn gltfldltvq qieylnktyn anvplilmds fntdddtqri
      181 irkykgidvd iytfnqscyp rinrdsllpi akhcdiaddi eawyppghgd fyesfrnsgl
      241 lkkflkegrd ycfisnidnl gatvdfkilk llldktetsp leflmevtdk tradvkggtl
      301 ikyedklrll eiaqvpkdha ddfksvktfk ffntnnlwik lsaiervlek nslnmeiivn
      361 nktfsnglni iqletavgaa mksfegsigi nvprsrflpv kktsdlmlvm snlytlrngs
      421 lvmspqrmfp ttpliklgdn hfskvkeflt rfpsipdlle ldhltvsgdv tfgkgvtlkg
      481 tviiianhge ridlpsgtil enkivsgnlr ildh
//
LOCUS       XP_034185632             510 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-glucose pyrophosphorylase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185632
VERSION     XP_034185632.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329741.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="UDP-glucose pyrophosphorylase isoform X2"
                     /calculated_mol_wt=57766
     Region          58..475
                     /region_name="UDPGP"
                     /note="UTP--glucose-1-phosphate uridylyltransferase;
                     pfam01704"
                     /db_xref="CDD:460300"
     CDS             1..510
                     /gene="UGP"
                     /coded_by="XM_034329741.2:119..1651"
                     /db_xref="GeneID:117606809"
ORIGIN      
        1 mlqvddrkar ghqrspsdtq afrertkrda lielqrelek legtattgev keevhrqfeg
       61 fthlfkrflq eegpslewdr iqklpddair dynslpmpeg eevrallnkl iviklngglg
      121 tsmgchgpks viavrngltf ldltvqqiey lnktynanvp lilmdsfntd ddtqriirky
      181 kgidvdiytf nqscyprinr dsllpiakhc diaddieawy ppghgdfyes frnsgllkkf
      241 lkegrdycfi snidnlgatv dfkilkllld ktetsplefl mevtdktrad vkggtlikye
      301 dklrlleiaq vpkdhaddfk svktfkffnt nnlwiklsai ervleknsln meiivnnktf
      361 snglniiqle tavgaamksf egsiginvpr srflpvkkts dlmlvmsnly tlrngslvms
      421 pqrmfpttpl iklgdnhfsk vkefltrfps ipdlleldhl tvsgdvtfgk gvtlkgtvii
      481 ianhgeridl psgtilenki vsgnlrildh
//
LOCUS       XP_034185634             414 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-glucose pyrophosphorylase isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185634
VERSION     XP_034185634.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329743.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="UDP-glucose pyrophosphorylase isoform X3"
                     /calculated_mol_wt=46417
     Region          2..379
                     /region_name="UDPGP"
                     /note="UTP--glucose-1-phosphate uridylyltransferase;
                     pfam01704"
                     /db_xref="CDD:460300"
     CDS             1..414
                     /gene="UGP"
                     /coded_by="XM_034329743.2:956..2200"
                     /db_xref="GeneID:117606809"
ORIGIN      
        1 mpegeevral lnklivikln gglgtsmgch gpksviavrn gltfldltvq qieylnktyn
       61 anvplilmds fntdddtqri irkykgidvd iytfnqscyp rinrdsllpi akhcdiaddi
      121 eawyppghgd fyesfrnsgl lkkflkegrd ycfisnidnl gatvdfkilk llldktetsp
      181 leflmevtdk tradvkggtl ikyedklrll eiaqvpkdha ddfksvktfk ffntnnlwik
      241 lsaiervlek nslnmeiivn nktfsnglni iqletavgaa mksfegsigi nvprsrflpv
      301 kktsdlmlvm snlytlrngs lvmspqrmfp ttpliklgdn hfskvkeflt rfpsipdlle
      361 ldhltvsgdv tfgkgvtlkg tviiianhge ridlpsgtil enkivsgnlr ildh
//
LOCUS       XP_034185690             423 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4A [Osmia lignaria
            lignaria].
ACCESSION   XP_034185690
VERSION     XP_034185690.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329799.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..423
                     /product="eukaryotic translation initiation factor 4A"
                     /calculated_mol_wt=48080
     Region          38..423
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(52,70,72,77,96..102)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350804"
     Site            order(53,63,66,71)
                     /site_type="other"
                     /note="eIF4G interface [polypeptide binding]"
                     /db_xref="CDD:350804"
     CDS             1..423
                     /gene="eIF4A"
                     /coded_by="XM_034329799.2:136..1407"
                     /db_xref="GeneID:117606828"
ORIGIN      
        1 mshtserrnd dqwagdskng pseseqqtyn gppgmdpdgi iesnwdvvvd nfdemnlkee
       61 llrgiyaygf ekpsaiqqra ilpcikghdv iaqaqsgtgk tatfsisilq qidtsikecq
      121 alilaptrel aqqiqkvvia lgdfmhaech aciggtnvre dmrkldqgvh ivvgtpgrvy
      181 dmisrralra nsiklfvlde ademlsrgfk eqihdvfkll pqevqvills atmpsdvldv
      241 skcfmrnpir ilvkkeeltl egikqffvfv ereewkfetl cdlydtlsit qavifcntrr
      301 kvdwltdnmr nrdftvsamh gdmeqkerdl imkqfrtgss rvlittdlla rgidvqqvsl
      361 vinydlpsnr enyihrigrg grfgrkgvai nfvteedkrt lkdieqfynt hidempmnva
      421 dli
//
LOCUS       XP_034185635             387 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606810 [Osmia lignaria lignaria].
ACCESSION   XP_034185635
VERSION     XP_034185635.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329744.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..387
                     /product="uncharacterized protein LOC117606810"
                     /calculated_mol_wt=44822
     Region          10..84
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); pfam07776"
                     /db_xref="CDD:462262"
     Region          122..142
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(127,129,131,133..134,137..138,141,155,157,161..162,
                     165..166,169,183,185,187,189..190,193..194)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          134..159
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          150..170
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          178..195
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          195..>337
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          220..240
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(225,227,229,231..232,235..236,239,253,255..257,
                     259..260,264)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          233..257
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          248..265
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..387
                     /gene="LOC117606810"
                     /coded_by="XM_034329744.2:134..1297"
                     /db_xref="GeneID:117606810"
ORIGIN      
        1 mmlhktnikl crlcgkekqq gtdlladkvk gtvlmsiink yfskevinis nsdafskyvc
       61 idceqkiyvf defylmvanv qkqlaapsle idfaedmlcq lkvnetvpks tlnkdgirkn
      121 vcpicaksfr cqahlirhkr ihtgqrpfvc nickmsfnqq eilmkhkerh egkkefrcan
      181 chqsfrykvs lkshmisfhm dmeqsvnnqn lptennsfmc secgkqfvtk yklqrhsrch
      241 tgerpyhctf clktfsqtgn lkvhqvkyhq isasltdlrr eaqydnqivd cdvpaelnnf
      301 htinmseiel qntinetins tqqsssyvtk iyenslyide eietildrdl gqleqnkyst
      361 nlqdklslcl kqpetpelln sllyddg
//
LOCUS       XP_034185691             350 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein sting [Osmia lignaria lignaria].
ACCESSION   XP_034185691
VERSION     XP_034185691.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329800.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185691.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="transmembrane protein sting"
                     /calculated_mol_wt=40295
     Region          84..347
                     /region_name="STING_C"
                     /note="C-terminal domain of STING; cl17092"
                     /db_xref="CDD:473064"
     Site            order(154,156..158,160..161,163..164,168,274,277..278,
                     310..311)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:213389"
     Site            order(161..162,165..166,242,244,267,270..271,274)
                     /site_type="other"
                     /note="cyclic di-GMP binding site [chemical binding]"
                     /db_xref="CDD:213389"
     CDS             1..350
                     /gene="Sting"
                     /coded_by="XM_034329800.2:401..1453"
                     /db_xref="GeneID:117606829"
ORIGIN      
        1 mdnkiklsli sctisgilik myfqatndns nltsskklfy iilcinaivv smnillrsyp
       61 riknahsnty ntdftnlmnn nlknyistsi fsiqlmiyvi ltvinyenly nyiqkfevli
      121 scmlilsalv qiidiidqdk espfdinsmq gldygtgmay syyygylrlv lpttgslnkg
      181 lieklenied ennirvavhk llilipessy vppdlkeasy kwmesartle eeirdragvk
      241 nriyrnslyk ifpdgdrnst pvyvpvegat plvtfgevqk hahpetkvys kyrkdvikrf
      301 heklkeliss dpecadkcel iyyndydeng mkvnvakvil drlqrsetyt
//
LOCUS       XP_034185636             338 aa            linear   INV 03-SEP-2025
DEFINITION  decapping enzyme, scavenger [Osmia lignaria lignaria].
ACCESSION   XP_034185636
VERSION     XP_034185636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="decapping enzyme, scavenger"
                     /calculated_mol_wt=38822
     Region          43..325
                     /region_name="COG5075"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227407"
     CDS             1..338
                     /gene="Dcps"
                     /coded_by="XM_034329745.2:287..1303"
                     /db_xref="GeneID:117606811"
ORIGIN      
        1 maelasdnnv decplakkak ldtenssent ksihendvtl pafdltkvlq tncmrkqiwv
       61 qgtfegydgp aiitlekqnf vddekalkef fskdtafqkl ysntiygnye cfpakkytgi
      121 namvihpats khidkftkke lymidetyel yqkitvpyie sssfstewih nilehkaeqd
      181 kivyedrnek tgfvlvndlk wdgqlntlkl ialplqkiks irelnashlp llknirdagi
      241 aaiakkfkls asqlriylhy qpsyyylhvh faylmydapg ifvekahlls mvirnielms
      301 dyytkavlsy vvaegdplyi kykeegtite lnskttev
//
LOCUS       XP_034185637             338 aa            linear   INV 03-SEP-2025
DEFINITION  decapping enzyme, scavenger [Osmia lignaria lignaria].
ACCESSION   XP_034185637
VERSION     XP_034185637.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329746.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="decapping enzyme, scavenger"
                     /calculated_mol_wt=38822
     Region          43..325
                     /region_name="COG5075"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227407"
     CDS             1..338
                     /gene="Dcps"
                     /coded_by="XM_034329746.2:266..1282"
                     /db_xref="GeneID:117606811"
ORIGIN      
        1 maelasdnnv decplakkak ldtenssent ksihendvtl pafdltkvlq tncmrkqiwv
       61 qgtfegydgp aiitlekqnf vddekalkef fskdtafqkl ysntiygnye cfpakkytgi
      121 namvihpats khidkftkke lymidetyel yqkitvpyie sssfstewih nilehkaeqd
      181 kivyedrnek tgfvlvndlk wdgqlntlkl ialplqkiks irelnashlp llknirdagi
      241 aaiakkfkls asqlriylhy qpsyyylhvh faylmydapg ifvekahlls mvirnielms
      301 dyytkavlsy vvaegdplyi kykeegtite lnskttev
//
LOCUS       XP_034185692             115 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4E binding protein thor
            [Osmia lignaria lignaria].
ACCESSION   XP_034185692
VERSION     XP_034185692.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329801.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="eukaryotic translation initiation factor 4E
                     binding protein thor"
                     /calculated_mol_wt=12459
     Region          11..115
                     /region_name="eIF_4EBP"
                     /note="Eukaryotic translation initiation factor 4E binding
                     protein (EIF4EBP); pfam05456"
                     /db_xref="CDD:461656"
     CDS             1..115
                     /gene="Thor"
                     /coded_by="XM_034329801.2:483..830"
                     /db_xref="GeneID:117606830"
ORIGIN      
        1 msaspiakqa tqsqnipskr ivindssqlp adysstpggt lfsttpggtr ivyereflmn
       61 lrnspisrtp prnmpsfate llknastnvv tsakpqinkd tpiieesaeq femdm
//
LOCUS       XP_034185693             115 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4E binding protein thor
            [Osmia lignaria lignaria].
ACCESSION   XP_034185693
VERSION     XP_034185693.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329802.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="eukaryotic translation initiation factor 4E
                     binding protein thor"
                     /calculated_mol_wt=12459
     Region          11..115
                     /region_name="eIF_4EBP"
                     /note="Eukaryotic translation initiation factor 4E binding
                     protein (EIF4EBP); pfam05456"
                     /db_xref="CDD:461656"
     CDS             1..115
                     /gene="Thor"
                     /coded_by="XM_034329802.2:534..881"
                     /db_xref="GeneID:117606830"
ORIGIN      
        1 msaspiakqa tqsqnipskr ivindssqlp adysstpggt lfsttpggtr ivyereflmn
       61 lrnspisrtp prnmpsfate llknastnvv tsakpqinkd tpiieesaeq femdm
//
LOCUS       XP_034185694             115 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4E binding protein thor
            [Osmia lignaria lignaria].
ACCESSION   XP_034185694
VERSION     XP_034185694.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329803.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="eukaryotic translation initiation factor 4E
                     binding protein thor"
                     /calculated_mol_wt=12459
     Region          11..115
                     /region_name="eIF_4EBP"
                     /note="Eukaryotic translation initiation factor 4E binding
                     protein (EIF4EBP); pfam05456"
                     /db_xref="CDD:461656"
     CDS             1..115
                     /gene="Thor"
                     /coded_by="XM_034329803.2:370..717"
                     /db_xref="GeneID:117606830"
ORIGIN      
        1 msaspiakqa tqsqnipskr ivindssqlp adysstpggt lfsttpggtr ivyereflmn
       61 lrnspisrtp prnmpsfate llknastnvv tsakpqinkd tpiieesaeq femdm
//
LOCUS       XP_034185695             115 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4E binding protein thor
            [Osmia lignaria lignaria].
ACCESSION   XP_034185695
VERSION     XP_034185695.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329804.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="eukaryotic translation initiation factor 4E
                     binding protein thor"
                     /calculated_mol_wt=12459
     Region          11..115
                     /region_name="eIF_4EBP"
                     /note="Eukaryotic translation initiation factor 4E binding
                     protein (EIF4EBP); pfam05456"
                     /db_xref="CDD:461656"
     CDS             1..115
                     /gene="Thor"
                     /coded_by="XM_034329804.2:440..787"
                     /db_xref="GeneID:117606830"
ORIGIN      
        1 msaspiakqa tqsqnipskr ivindssqlp adysstpggt lfsttpggtr ivyereflmn
       61 lrnspisrtp prnmpsfate llknastnvv tsakpqinkd tpiieesaeq femdm
//
LOCUS       XP_034185696             115 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4E binding protein thor
            [Osmia lignaria lignaria].
ACCESSION   XP_034185696
VERSION     XP_034185696.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329805.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="eukaryotic translation initiation factor 4E
                     binding protein thor"
                     /calculated_mol_wt=12459
     Region          11..115
                     /region_name="eIF_4EBP"
                     /note="Eukaryotic translation initiation factor 4E binding
                     protein (EIF4EBP); pfam05456"
                     /db_xref="CDD:461656"
     CDS             1..115
                     /gene="Thor"
                     /coded_by="XM_034329805.2:342..689"
                     /db_xref="GeneID:117606830"
ORIGIN      
        1 msaspiakqa tqsqnipskr ivindssqlp adysstpggt lfsttpggtr ivyereflmn
       61 lrnspisrtp prnmpsfate llknastnvv tsakpqinkd tpiieesaeq femdm
//
LOCUS       XP_034190558             701 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608911 [Osmia lignaria lignaria].
ACCESSION   XP_034190558
VERSION     XP_034190558.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334667.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190558.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..701
                     /product="uncharacterized protein LOC117608911"
                     /calculated_mol_wt=79124
     Region          <319..567
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..701
                     /gene="LOC117608911"
                     /coded_by="XM_034334667.2:221..2326"
                     /db_xref="GeneID:117608911"
ORIGIN      
        1 mihskatavl mrlkemdrky kikhkeekrt hslvstessl entpksnnsk lvhegneias
       61 kvsaeskmem qgsgtnnvns sdedvhskks pkisprtrte vslsveiegn irsasrtees
      121 lrtvsnlesd lktnsqirdd vllsedsrrh geessaatif skrsaapeti sevvksrneg
      181 ksnttnpedd ttideviris derseiisel snpidkdeda snvssmdeqk lqyendtfee
      241 asnssgssss telqlkksse elagetvisg vkeikitshk qaeiiqekvi ndekdkeiie
      301 liapkiiksn secdvgldee lsnyvrtten vdevlpinll kpskqtttgk khvrksrkqr
      361 kssnenteek tedlsvvsdd herstkvnnn edallkenss smivksasne gkivketlkv
      421 kkeslsneqt pveieeeanr epetissekl ltetpkktda tstlrelnkd ainaivrrhr
      481 iqtinkitdk pryknyktvv klpsaesevp enmeknesks lenirtdssh nqrvrgrrak
      541 vkrvskaska rkgsyqgkve ekqskfecrq srhlrkqaaa lrmqqeredi rnyllelect
      601 rlefgpgemt satippfkpl efpkisafkk peledsnlpt dgivalqeki ltirqwlkdq
      661 yflyrdyskl aqtvnakyip asledakkti rqlqsaties k
//
LOCUS       XP_034190559             468 aa            linear   INV 03-SEP-2025
DEFINITION  DNA damage inducible 1 homolog rngo isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190559
VERSION     XP_034190559.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334668.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..468
                     /product="DNA damage inducible 1 homolog rngo isoform X1"
                     /calculated_mol_wt=51959
     Region          1..72
                     /region_name="Ubl_Ddi1_like"
                     /note="ubiquitin-like (Ubl) domain found in the eukaryotic
                     Ddi1 family; cd01796"
                     /db_xref="CDD:340494"
     Region          213..336
                     /region_name="RP_DDI"
                     /note="retropepsin-like domain of DNA damage inducible
                     protein; cd05479"
                     /db_xref="CDD:133146"
     Site            245..247
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133146"
     Site            245
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133146"
     Region          427..455
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     CDS             1..468
                     /gene="rngo"
                     /coded_by="XM_034334668.2:300..1706"
                     /db_xref="GeneID:117608912"
ORIGIN      
        1 mkvtvttlsd difvldvied melssfkalc eiecnvpthe mmiafnglpl mddkkslkdh
       61 giqdgdvvil qhmhqsgadl nlhpfngaip mldfstirvp gapsssrqpg psasaiariq
      121 npsseddpei irqmvlanpd qlallsqnnp elasalisgn letfstvlke qikfreerea
      181 qrlrmmnadp fdtetqrfia edirqkniea nmeaameynp etfgsvvmly inckvngfpv
      241 kafidtgaqs timsdacaer chlmrlvdsr wagiahgvgt qriigrihmv qiqigndhlt
      301 tsftvlaeqs mdmllgldml krhqccidlk tnvltigttg aetpflaege lpewlrlsgy
      361 sdmsmydied dkkgdkkgpq rseedssrdv kkqrrqnedq asnnasgtss rigaiettvf
      421 ahdpfteetv keieslgfnr vqviaelrkf ngdkakatds lftksirl
//
LOCUS       XP_034190560             404 aa            linear   INV 03-SEP-2025
DEFINITION  DNA damage inducible 1 homolog rngo isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190560
VERSION     XP_034190560.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334669.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="DNA damage inducible 1 homolog rngo isoform X2"
                     /calculated_mol_wt=44861
     Region          1..72
                     /region_name="Ubl_Ddi1_like"
                     /note="ubiquitin-like (Ubl) domain found in the eukaryotic
                     Ddi1 family; cd01796"
                     /db_xref="CDD:340494"
     Region          213..336
                     /region_name="RP_DDI"
                     /note="retropepsin-like domain of DNA damage inducible
                     protein; cd05479"
                     /db_xref="CDD:133146"
     Site            245..247
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133146"
     Site            245
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133146"
     CDS             1..404
                     /gene="rngo"
                     /coded_by="XM_034334669.2:303..1517"
                     /db_xref="GeneID:117608912"
ORIGIN      
        1 mkvtvttlsd difvldvied melssfkalc eiecnvpthe mmiafnglpl mddkkslkdh
       61 giqdgdvvil qhmhqsgadl nlhpfngaip mldfstirvp gapsssrqpg psasaiariq
      121 npsseddpei irqmvlanpd qlallsqnnp elasalisgn letfstvlke qikfreerea
      181 qrlrmmnadp fdtetqrfia edirqkniea nmeaameynp etfgsvvmly inckvngfpv
      241 kafidtgaqs timsdacaer chlmrlvdsr wagiahgvgt qriigrihmv qiqigndhlt
      301 tsftvlaeqs mdmllgldml krhqccidlk tnvltigttg aetpflaege lpewlrlsgy
      361 sdmsmydied dkkgdkkgpq rseedssrdv kkqrrqngkg sskq
//
LOCUS       XP_034190561             378 aa            linear   INV 03-SEP-2025
DEFINITION  DNA damage inducible 1 homolog rngo isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190561
VERSION     XP_034190561.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334670.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..378
                     /product="DNA damage inducible 1 homolog rngo isoform X3"
                     /calculated_mol_wt=41998
     Region          123..246
                     /region_name="RP_DDI"
                     /note="retropepsin-like domain of DNA damage inducible
                     protein; cd05479"
                     /db_xref="CDD:133146"
     Site            155..157
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133146"
     Site            155
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133146"
     Region          337..365
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     CDS             1..378
                     /gene="rngo"
                     /coded_by="XM_034334670.2:152..1288"
                     /db_xref="GeneID:117608912"
ORIGIN      
        1 mldfstirvp gapsssrqpg psasaiariq npsseddpei irqmvlanpd qlallsqnnp
       61 elasalisgn letfstvlke qikfreerea qrlrmmnadp fdtetqrfia edirqkniea
      121 nmeaameynp etfgsvvmly inckvngfpv kafidtgaqs timsdacaer chlmrlvdsr
      181 wagiahgvgt qriigrihmv qiqigndhlt tsftvlaeqs mdmllgldml krhqccidlk
      241 tnvltigttg aetpflaege lpewlrlsgy sdmsmydied dkkgdkkgpq rseedssrdv
      301 kkqrrqnedq asnnasgtss rigaiettvf ahdpfteetv keieslgfnr vqviaelrkf
      361 ngdkakatds lftksirl
//
LOCUS       XP_034190562             403 aa            linear   INV 03-SEP-2025
DEFINITION  calreticulin [Osmia lignaria lignaria].
ACCESSION   XP_034190562
VERSION     XP_034190562.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334671.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="calreticulin"
                     /calculated_mol_wt=46927
     Region          22..331
                     /region_name="Calreticulin"
                     /note="Calreticulin family; pfam00262"
                     /db_xref="CDD:459737"
     CDS             1..403
                     /gene="Calr"
                     /coded_by="XM_034334671.2:78..1289"
                     /db_xref="GeneID:117608913"
ORIGIN      
        1 mrsfcsflll lavtvvtaet yfeekfldds weknwvyseh pgkefgkfvl shgkfwndre
       61 ndkgiqtsqd arfyalsrkf kpfsnkdkpl viqfsvkheq nidcgggyvk vfdcsldqkd
      121 mhgetpyeim fgpdicgpgt kkvhvifsyk gknllinkdi rckddiythl ytlivspdnt
      181 ykvlidneev esgeleadwd flppkkikdp sqskpddwdd kptipdpddk kpedwdkpeh
      241 ipdpeatkpe dwddemdgew eapmidnpey kgewkpkqid npnykgpwih peidnpeytp
      301 dpelykrdei caigfdlwqv ksgtifdnvl iaddpevark fgeevwkaty egekkmkeal
      361 deeerkqrek eskenednkd deededgdee dntvpdaeeh del
//
LOCUS       XP_034190564             391 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolaminephosphotransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190564
VERSION     XP_034190564.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334673.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..391
                     /product="ethanolaminephosphotransferase 1"
                     /calculated_mol_wt=44942
     Region          11..391
                     /region_name="PRK10832"
                     /note="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase; cl00453"
                     /db_xref="CDD:469774"
     CDS             1..391
                     /gene="LOC117608914"
                     /coded_by="XM_034334673.2:633..1808"
                     /db_xref="GeneID:117608914"
ORIGIN      
        1 myqklklevq ylteqhltgf enykynsvdt splsvyimhp fwnkvvqycp kwvapnvltf
       61 sgflftvfnf tmfasydyyf yassddkpqy lpiprwvfal gafnvfmayt ldgidgkqar
      121 rtqtsgplge lfdhgldswt tmlitvcmfs vfgrtdhsvs plrmyfilwn vfinfylthw
      181 ekyntgvlyl pwgydlsmvg ttivfiitsi gghrawkfvl pggisvgvmf elvlyvtaiv
      241 sslpvvlwni yksyrdktgk mrtfldairp liplavffvi stiwvihsps niiekdpriv
      301 fliigtifsn iccrlivsqm sntacemlsw ilfpiafaaa fsfilpstdl vvayivaila
      361 llahihygtc vvrqmcrhfr ihtfrikdra d
//
LOCUS       XP_034190565             388 aa            linear   INV 03-SEP-2025
DEFINITION  inositol 2-dehydrogenase [Osmia lignaria lignaria].
ACCESSION   XP_034190565
VERSION     XP_034190565.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334674.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190565.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..388
                     /product="inositol 2-dehydrogenase"
                     /calculated_mol_wt=43627
     Region          43..375
                     /region_name="myo_inos_iolG"
                     /note="inositol 2-dehydrogenase; TIGR04380"
                     /db_xref="CDD:275173"
     CDS             1..388
                     /gene="LOC117608915"
                     /coded_by="XM_034334674.2:168..1334"
                     /db_xref="GeneID:117608915"
ORIGIN      
        1 matakfkeas pytkpqptpp pqdylyqkym edialknear hktiklalfg vgragtihls
       61 nvvanprvkl lyvvddvesk weairqhwhl ddvtflnikq sdkvyndpnv davivaspty
      121 thetivrkal eakkavfcek pvaedrvntm kcyetakkvg kplfcafnrr fdpsyssvke
      181 rvrkgevghv htiktvsrds plptldylkl sggifhdcmv hdldmitwvl geypvkvavq
      241 asanipeikq igdfdtvtvs lyfpsgtlgm idlsrnsnyg ydqrlevfgp kgmikadneq
      301 pmhcvntqyg lqgsnsppiw ysfpsrfmng yrrelehfvd ivlgmdessv laketlavsk
      361 lasaceesar sgkmveikwa vnelpevl
//
LOCUS       XP_076545776             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545776
VERSION     XP_076545776.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689661.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689661.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545777             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545777
VERSION     XP_076545777.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689662.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689662.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545778             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545778
VERSION     XP_076545778.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689663.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689663.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545779             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545779
VERSION     XP_076545779.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689664.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689664.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545780             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545780
VERSION     XP_076545780.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689665.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689665.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545781             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545781
VERSION     XP_076545781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689666.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689666.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545782             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545782
VERSION     XP_076545782.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689667.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689667.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545783             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545783
VERSION     XP_076545783.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689668.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689668.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545784             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545784
VERSION     XP_076545784.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689669.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689669.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545785             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545785
VERSION     XP_076545785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689670.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689670.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545786             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545786
VERSION     XP_076545786.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689671.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689671.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545787             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545787
VERSION     XP_076545787.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689672.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689672.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545788             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545788
VERSION     XP_076545788.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689673.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689673.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545789             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545789
VERSION     XP_076545789.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689674.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689674.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_076545790             295 aa            linear   INV 03-SEP-2025
DEFINITION  putative enoyl-CoA hydratase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_076545790
VERSION     XP_076545790.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689675.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="putative enoyl-CoA hydratase, mitochondrial"
                     /calculated_mol_wt=31754
     Region          37..294
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(63,65,97,101..105,142,144..146,168..169,172)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(103,146)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(130,134,155..158,170..173,179,181..183,185..186,
                     191..192,194..195,197..198,201,212,215,230,233..234)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..295
                     /gene="LOC117608920"
                     /coded_by="XM_076689675.1:140..1027"
                     /db_xref="GeneID:117608920"
ORIGIN      
        1 mattrvgqil ftkslqigrk pqyfasnvkf yccqvsnyey ikvetvgekk nvglvtlnrp
       61 kalnalcdkl mtevtdavsk fdnndgigai vltgsekafa agadikemqn qtysqtirgn
      121 fltswdgvsk aikpviaavn gyalgggcel ammcdiiyag dkakfgqpei aigtipgagg
      181 tqrltraigk skamemvltg nqitaeeaek nglvskvlpa gqlvaeaikl aekiashsqi
      241 tvamakecvn tayettlkeg lhfekrmfhg tfattdrkeg mtafvekrpp kftne
//
LOCUS       XP_034190567             217 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608918 [Osmia lignaria lignaria].
ACCESSION   XP_034190567
VERSION     XP_034190567.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334676.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="uncharacterized protein LOC117608918"
                     /calculated_mol_wt=24394
     CDS             1..217
                     /gene="LOC117608918"
                     /coded_by="XM_034334676.2:1..654"
                     /db_xref="GeneID:117608918"
ORIGIN      
        1 marqqtadrn iphlrdifgf gskwentrip aeekaaghds avsvgstsge eespknskkk
       61 ksrrrdnskt ltesdvkhle rhlsmkktir kkimrdlqqa fvedpnefrv ddippeqlka
      121 einiqslsfg tpsqkqgrtr gnaentfldm lragglekns tdgdrdsghg gsptretsnv
      181 qdtddessyp ydtpvatpsk ksgnfwrrft mksrnkr
//
LOCUS       XP_034190568             215 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117608919 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190568
VERSION     XP_034190568.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334677.2
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190568.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117608919"
                     /calculated_mol_wt=23910
     Region          65..>168
                     /region_name="ZP"
                     /note="Zona pellucida (ZP) domain; smart00241"
                     /db_xref="CDD:214579"
     CDS             1..215
                     /gene="LOC117608919"
                     /coded_by="XM_034334677.2:1..648"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:117608919"
ORIGIN      
        1 mnlnspsvhg rssgnfyvmr lplhallffi faleqrlaee iwlqdlsmdm eigastegyd
       61 qvslhcgaek mtvnlktsen ftgviytqgs fysrqapcfl dpahggnftl nipfdqcdte
      121 nsddkykntl viqhddelit pgdaafvlec dfskptyvtv saelnepdkr lvrssislvd
      181 adpardktkr tacvksdtne vvfvpnsilk lndel
//
LOCUS       XP_034190332              94 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil-helix-coiled-coil-helix domain-containing protein 7
            [Osmia lignaria lignaria].
ACCESSION   XP_034190332
VERSION     XP_034190332.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334441.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..94
                     /product="coiled-coil-helix-coiled-coil-helix
                     domain-containing protein 7"
                     /calculated_mol_wt=11328
     Region          21..>63
                     /region_name="Cyt_c_Oxidase_VIb"
                     /note="Cytochrome c oxidase subunit VIb. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cl02794"
                     /db_xref="CDD:445926"
     CDS             1..94
                     /gene="LOC117608812"
                     /coded_by="XM_034334441.2:158..442"
                     /db_xref="GeneID:117608812"
ORIGIN      
        1 mnkfneraek nlnarkqftr nqelenpcwk ehnislkcld knnydyekct lqfenyktck
       61 sfwkhvvtdr tirniqpalp lpedrekvka qyfs
//
LOCUS       XP_076545791              72 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome c oxidase subunit 7C [Osmia lignaria lignaria].
ACCESSION   XP_076545791
VERSION     XP_076545791.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689676.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..72
                     /product="cytochrome c oxidase subunit 7C"
                     /calculated_mol_wt=8116
     Region          28..72
                     /region_name="Cyt_c_Oxidase_VIIc"
                     /note="Cytochrome c oxidase subunit VIIc. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cl02797"
                     /db_xref="CDD:445929"
     Site            order(28..30,32,34..35,37,40..41,44,54,57..59,62..63,65,
                     68..69,72)
                     /site_type="other"
                     /note="Subunit VIIc/I interface [polypeptide binding]"
                     /db_xref="CDD:238469"
     Site            order(44,59,63,67)
                     /site_type="other"
                     /note="Subunit VIIc/VIIIb interface [polypeptide binding]"
                     /db_xref="CDD:238469"
     Site            order(68,72)
                     /site_type="other"
                     /note="Subunit VIIc/VIIa interface [polypeptide binding]"
                     /db_xref="CDD:238469"
     CDS             1..72
                     /gene="COX7C"
                     /coded_by="XM_076689676.1:75..293"
                     /db_xref="GeneID:143305571"
ORIGIN      
        1 msapraafqq clrsfrtsai rrsghdepeg fpgsnlpfsi hghrykllay fiaffgvpfa
       61 lpffvvrhhl lk
//
LOCUS       XP_034185376            1652 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606699 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185376
VERSION     XP_034185376.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185376.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1652
                     /product="uncharacterized protein LOC117606699 isoform X1"
                     /calculated_mol_wt=185993
     Region          46..108
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          <597..872
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          1458..>1573
                     /region_name="PRK11907"
                     /note="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /db_xref="CDD:237019"
     Region          1586..1641
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..1652
                     /gene="LOC117606699"
                     /coded_by="XM_034329485.2:698..5656"
                     /db_xref="GeneID:117606699"
ORIGIN      
        1 mnlalhgfll hvflsilnlq hgfvlcsage pgyldfdnlp etnfscqgkv iggyyadvea
       61 gcqmfhvcti gqkdlvsdei mdikflclng tvfdqetrvc ervdevdcsk serfynlnle
      121 lygnnavtlr lkpyslhens eddvdpsdhl ddrqrttsar pttttttstt skpnkpsttp
      181 ssnsyshptg ypqhfqpqpp fpqvhtsqsk slyddknggy hhqyifhnge rnnnqqatsy
      241 qlfsnqgvss ttvqtpqvhq irfsstpspq iihnepstva plfhtpssti qtllssngns
      301 pslinpifhs hgivstteqf sihsnnprdt seyrdngeqs irpleavqst nkgkvsklti
      361 spvptpseps rsgqtkssqs sqqqrisssf lptptteett arsfyptprs stkssessqs
      421 sseqvtqhih vlppvqipql kphqitinlp ppdiqrivqn pssllpsqsr vivtakasvs
      481 desgrplntt qlvtlplpti pasyddykeg desfdpfyrd vpkirnsrra yggvrrgriv
      541 krsrrsvgqt knsmvsfvyg getnrrhdiq tvnaaekeri kdrvtveddn mdfksikesl
      601 mrfrdilfge eftdetlrsi fedspsqnks sntkakgkkk esledlepra sknfkareye
      661 ddtdegsenk knkaksaski dtkisdaeey idiedneesk sdskngnied penekeeqse
      721 vsydyndetd ekvestttee peldiiildp neylrlksse qqstmnpvee elsttivpvl
      781 stsvqsielk kdsdflkdpk kpkdqdstpe inkqpkvned deekkpeqev kkekpkrkss
      841 rirsggkvss rkketksesd kpvessddki rnqkvettts aadseseqid khifdepesq
      901 tskeidtrav gnhnthtksl qggkdqgtgk enrkgdiney etlekqpnkl ekdqekvdkh
      961 kkevedeees tistldedkp eedeekvees kpndesettt eentslektd ykeessiehe
     1021 sveeelttif ssedveegsd emvksshhek skkfkdssre vslrkkekkr kneeeeeaei
     1081 esqeystvks eeinsketsm eyhdsteasf estheinade eypesyedst plselsseei
     1141 pssreevaee peeeewasdq evstegvlkf tdelpkekke dtsegkksse svdnvghdyv
     1201 ddnyepenyk erdssdsnsl snekmnketm sdeksesktg kpeedkeeev kdelqkdqas
     1261 eiedpkesst eeadlqttts ttttttsttt ttttttvrpt ppklfkpisa rksynyippt
     1321 ttpnpviikp rlsllnpkpa kppksyndla pkpvirkfpl ltrksattms tttirgedst
     1381 eipemtstev sststevsks eenvleskld pvsqpvsepp kdhaplknkn dqdsspseqk
     1441 sptnlkkeed edftpypvar lstmatllee ilkdtestti eaentttaks ftlidveits
     1501 ttaapvssts teadhettta ksltkdeasi apstkstyqe vteasplpet stmpettstp
     1561 seevesttmr lltrkmvglr rnqggfncle kemyrfygdn rdcrlfhycs pgftsrqvld
     1621 frfvceegta fdeetqscrh nvrnkrcpdr qw
//
LOCUS       XP_034185378            1648 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606699 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185378
VERSION     XP_034185378.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329487.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185378.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1648
                     /product="uncharacterized protein LOC117606699 isoform X2"
                     /calculated_mol_wt=185578
     Region          46..104
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          <593..868
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          1454..>1569
                     /region_name="PRK11907"
                     /note="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /db_xref="CDD:237019"
     Region          1582..1637
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..1648
                     /gene="LOC117606699"
                     /coded_by="XM_034329487.2:698..5644"
                     /db_xref="GeneID:117606699"
ORIGIN      
        1 mnlalhgfll hvflsilnlq hgfvlcsage pgyldfdnlp etnfscqgkv iggyyadvea
       61 gcqmfhvcti gqkdeimdik flclngtvfd qetrvcervd evdcskserf ynlnlelygn
      121 navtlrlkpy slhenseddv dpsdhlddrq rttsarpttt tttsttskpn kpsttpssns
      181 yshptgypqh fqpqppfpqv htsqskslyd dknggyhhqy ifhngernnn qqatsyqlfs
      241 nqgvssttvq tpqvhqirfs stpspqiihn epstvaplfh tpsstiqtll ssngnspsli
      301 npifhshgiv stteqfsihs nnprdtseyr dngeqsirpl eavqstnkgk vskltispvp
      361 tpsepsrsgq tkssqssqqq risssflptp tteettarsf yptprsstks sessqssseq
      421 vtqhihvlpp vqipqlkphq itinlpppdi qrivqnpssl lpsqsrvivt akasvsdesg
      481 rplnttqlvt lplptipasy ddykegdesf dpfyrdvpki rnsrrayggv rrgrivkrsr
      541 rsvgqtknsm vsfvyggetn rrhdiqtvna aekerikdrv tveddnmdfk sikeslmrfr
      601 dilfgeeftd etlrsifeds psqnkssntk akgkkkesle dleprasknf kareyeddtd
      661 egsenkknka ksaskidtki sdaeeyidie dneesksdsk ngniedpene keeqsevsyd
      721 yndetdekve sttteepeld iiildpneyl rlksseqqst mnpveeelst tivpvlstsv
      781 qsielkkdsd flkdpkkpkd qdstpeinkq pkvneddeek kpeqevkkek pkrkssrirs
      841 ggkvssrkke tksesdkpve ssddkirnqk vetttsaads eseqidkhif depesqtske
      901 idtravgnhn thtkslqggk dqgtgkenrk gdineyetle kqpnklekdq ekvdkhkkev
      961 edeeestist ldedkpeede ekveeskpnd esettteent slektdykee ssiehesvee
     1021 elttifssed veegsdemvk sshhekskkf kdssrevslr kkekkrknee eeeaeiesqe
     1081 ystvkseein sketsmeyhd steasfesth einadeeype syedstplse lsseeipssr
     1141 eevaeepeee ewasdqevst egvlkftdel pkekkedtse gkkssesvdn vghdyvddny
     1201 epenykerds sdsnslsnek mnketmsdek sesktgkpee dkeeevkdel qkdqaseied
     1261 pkessteead lqtttstttt ttsttttttt ttvrptppkl fkpisarksy nyipptttpn
     1321 pviikprlsl lnpkpakppk syndlapkpv irkfplltrk sattmsttti rgedsteipe
     1381 mtstevssts tevskseenv leskldpvsq pvseppkdha plknkndqds spseqksptn
     1441 lkkeededft pypvarlstm atlleeilkd testtieaen tttaksftli dveitsttaa
     1501 pvsststead hetttakslt kdeasiapst kstyqevtea splpetstmp ettstpseev
     1561 esttmrlltr kmvglrrnqg gfnclekemy rfygdnrdcr lfhycspgft srqvldfrfv
     1621 ceegtafdee tqscrhnvrn krcpdrqw
//
LOCUS       XP_034185379            1647 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606699 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185379
VERSION     XP_034185379.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329488.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185379.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1647
                     /product="uncharacterized protein LOC117606699 isoform X3"
                     /calculated_mol_wt=185335
     Region          46..108
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          <592..867
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          1453..>1568
                     /region_name="PRK11907"
                     /note="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /db_xref="CDD:237019"
     Region          1581..1636
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..1647
                     /gene="LOC117606699"
                     /coded_by="XM_034329488.2:698..5641"
                     /db_xref="GeneID:117606699"
ORIGIN      
        1 mnlalhgfll hvflsilnlq hgfvlcsage pgyldfdnlp etnfscqgkv iggyyadvea
       61 gcqmfhvcti gqkdlvsdei mdikflclng tvfdqetrvc ervdevdcsk serfynlnle
      121 lygnnavtls lhenseddvd psdhlddrqr ttsarptttt ttsttskpnk psttpssnsy
      181 shptgypqhf qpqppfpqvh tsqskslydd knggyhhqyi fhngernnnq qatsyqlfsn
      241 qgvssttvqt pqvhqirfss tpspqiihne pstvaplfht psstiqtlls sngnspslin
      301 pifhshgivs tteqfsihsn nprdtseyrd ngeqsirple avqstnkgkv skltispvpt
      361 psepsrsgqt kssqssqqqr isssflptpt teettarsfy ptprsstkss essqssseqv
      421 tqhihvlppv qipqlkphqi tinlpppdiq rivqnpssll psqsrvivta kasvsdesgr
      481 plnttqlvtl plptipasyd dykegdesfd pfyrdvpkir nsrrayggvr rgrivkrsrr
      541 svgqtknsmv sfvyggetnr rhdiqtvnaa ekerikdrvt veddnmdfks ikeslmrfrd
      601 ilfgeeftde tlrsifedsp sqnkssntka kgkkkesled leprasknfk areyeddtde
      661 gsenkknkak saskidtkis daeeyidied neesksdskn gniedpenek eeqsevsydy
      721 ndetdekves ttteepeldi iildpneylr lksseqqstm npveeelstt ivpvlstsvq
      781 sielkkdsdf lkdpkkpkdq dstpeinkqp kvneddeekk peqevkkekp krkssrirsg
      841 gkvssrkket ksesdkpves sddkirnqkv etttsaadse seqidkhifd epesqtskei
      901 dtravgnhnt htkslqggkd qgtgkenrkg dineyetlek qpnklekdqe kvdkhkkeve
      961 deeestistl dedkpeedee kveeskpnde settteents lektdykees siehesveee
     1021 lttifssedv eegsdemvks shhekskkfk dssrevslrk kekkrkneee eeaeiesqey
     1081 stvkseeins ketsmeyhds teasfesthe inadeeypes yedstplsel sseeipssre
     1141 evaeepeeee wasdqevste gvlkftdelp kekkedtseg kkssesvdnv ghdyvddnye
     1201 penykerdss dsnslsnekm nketmsdeks esktgkpeed keeevkdelq kdqaseiedp
     1261 kessteeadl qtttsttttt tstttttttt tvrptppklf kpisarksyn yipptttpnp
     1321 viikprlsll npkpakppks yndlapkpvi rkfplltrks attmstttir gedsteipem
     1381 tstevsstst evskseenvl eskldpvsqp vseppkdhap lknkndqdss pseqksptnl
     1441 kkeededftp ypvarlstma tlleeilkdt esttieaent ttaksftlid veitsttaap
     1501 vsststeadh etttaksltk deasiapstk styqevteas plpetstmpe ttstpseeve
     1561 sttmrlltrk mvglrrnqgg fnclekemyr fygdnrdcrl fhycspgfts rqvldfrfvc
     1621 eegtafdeet qscrhnvrnk rcpdrqw
//
LOCUS       XP_034185380            1643 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606699 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185380
VERSION     XP_034185380.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185380.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1643
                     /product="uncharacterized protein LOC117606699 isoform X4"
                     /calculated_mol_wt=184920
     Region          46..104
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          <588..863
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          1449..>1564
                     /region_name="PRK11907"
                     /note="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /db_xref="CDD:237019"
     Region          1577..1632
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..1643
                     /gene="LOC117606699"
                     /coded_by="XM_034329489.2:698..5629"
                     /db_xref="GeneID:117606699"
ORIGIN      
        1 mnlalhgfll hvflsilnlq hgfvlcsage pgyldfdnlp etnfscqgkv iggyyadvea
       61 gcqmfhvcti gqkdeimdik flclngtvfd qetrvcervd evdcskserf ynlnlelygn
      121 navtlslhen seddvdpsdh lddrqrttsa rpttttttst tskpnkpstt pssnsyshpt
      181 gypqhfqpqp pfpqvhtsqs kslyddkngg yhhqyifhng ernnnqqats yqlfsnqgvs
      241 sttvqtpqvh qirfsstpsp qiihnepstv aplfhtpsst iqtllssngn spslinpifh
      301 shgivstteq fsihsnnprd tseyrdngeq sirpleavqs tnkgkvsklt ispvptpsep
      361 srsgqtkssq ssqqqrisss flptptteet tarsfyptpr sstkssessq ssseqvtqhi
      421 hvlppvqipq lkphqitinl pppdiqrivq npssllpsqs rvivtakasv sdesgrplnt
      481 tqlvtlplpt ipasyddyke gdesfdpfyr dvpkirnsrr ayggvrrgri vkrsrrsvgq
      541 tknsmvsfvy ggetnrrhdi qtvnaaeker ikdrvtvedd nmdfksikes lmrfrdilfg
      601 eeftdetlrs ifedspsqnk ssntkakgkk kesledlepr asknfkarey eddtdegsen
      661 kknkaksask idtkisdaee yidiednees ksdskngnie dpenekeeqs evsydyndet
      721 dekvesttte epeldiiild pneylrlkss eqqstmnpve eelsttivpv lstsvqsiel
      781 kkdsdflkdp kkpkdqdstp einkqpkvne ddeekkpeqe vkkekpkrks srirsggkvs
      841 srkketkses dkpvessddk irnqkvettt saadseseqi dkhifdepes qtskeidtra
      901 vgnhnthtks lqggkdqgtg kenrkgdine yetlekqpnk lekdqekvdk hkkevedeee
      961 stistldedk peedeekvee skpndesett teentslekt dykeessieh esveeeltti
     1021 fssedveegs demvksshhe kskkfkdssr evslrkkekk rkneeeeeae iesqeystvk
     1081 seeinskets meyhdsteas festheinad eeypesyeds tplselssee ipssreevae
     1141 epeeeewasd qevstegvlk ftdelpkekk edtsegkkss esvdnvghdy vddnyepeny
     1201 kerdssdsns lsnekmnket msdekseskt gkpeedkeee vkdelqkdqa seiedpkess
     1261 teeadlqttt sttttttstt tttttttvrp tppklfkpis arksynyipp tttpnpviik
     1321 prlsllnpkp akppksyndl apkpvirkfp lltrksattm stttirgeds teipemtste
     1381 vsststevsk seenvleskl dpvsqpvsep pkdhaplknk ndqdsspseq ksptnlkkee
     1441 dedftpypva rlstmatlle eilkdtestt ieaentttak sftlidveit sttaapvsst
     1501 steadhettt aksltkdeas iapstkstyq evteasplpe tstmpettst pseevesttm
     1561 rlltrkmvgl rrnqggfncl ekemyrfygd nrdcrlfhyc spgftsrqvl dfrfvceegt
     1621 afdeetqscr hnvrnkrcpd rqw
//
LOCUS       XP_034185381            1642 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606699 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185381
VERSION     XP_034185381.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329490.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185381.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1642
                     /product="uncharacterized protein LOC117606699 isoform X5"
                     /calculated_mol_wt=184754
     Region          46..108
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          <587..862
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     Region          1448..>1563
                     /region_name="PRK11907"
                     /note="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /db_xref="CDD:237019"
     Region          1576..1631
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..1642
                     /gene="LOC117606699"
                     /coded_by="XM_034329490.2:698..5626"
                     /db_xref="GeneID:117606699"
ORIGIN      
        1 mnlalhgfll hvflsilnlq hgfvlcsage pgyldfdnlp etnfscqgkv iggyyadvea
       61 gcqmfhvcti gqkdlvsdei mdikflclng tvfdqetrvc ervdevdcsk serfynlnle
      121 lygnnavtls eddvdpsdhl ddrqrttsar pttttttstt skpnkpsttp ssnsyshptg
      181 ypqhfqpqpp fpqvhtsqsk slyddknggy hhqyifhnge rnnnqqatsy qlfsnqgvss
      241 ttvqtpqvhq irfsstpspq iihnepstva plfhtpssti qtllssngns pslinpifhs
      301 hgivstteqf sihsnnprdt seyrdngeqs irpleavqst nkgkvsklti spvptpseps
      361 rsgqtkssqs sqqqrisssf lptptteett arsfyptprs stkssessqs sseqvtqhih
      421 vlppvqipql kphqitinlp ppdiqrivqn pssllpsqsr vivtakasvs desgrplntt
      481 qlvtlplpti pasyddykeg desfdpfyrd vpkirnsrra yggvrrgriv krsrrsvgqt
      541 knsmvsfvyg getnrrhdiq tvnaaekeri kdrvtveddn mdfksikesl mrfrdilfge
      601 eftdetlrsi fedspsqnks sntkakgkkk esledlepra sknfkareye ddtdegsenk
      661 knkaksaski dtkisdaeey idiedneesk sdskngnied penekeeqse vsydyndetd
      721 ekvestttee peldiiildp neylrlksse qqstmnpvee elsttivpvl stsvqsielk
      781 kdsdflkdpk kpkdqdstpe inkqpkvned deekkpeqev kkekpkrkss rirsggkvss
      841 rkketksesd kpvessddki rnqkvettts aadseseqid khifdepesq tskeidtrav
      901 gnhnthtksl qggkdqgtgk enrkgdiney etlekqpnkl ekdqekvdkh kkevedeees
      961 tistldedkp eedeekvees kpndesettt eentslektd ykeessiehe sveeelttif
     1021 ssedveegsd emvksshhek skkfkdssre vslrkkekkr kneeeeeaei esqeystvks
     1081 eeinsketsm eyhdsteasf estheinade eypesyedst plselsseei pssreevaee
     1141 peeeewasdq evstegvlkf tdelpkekke dtsegkksse svdnvghdyv ddnyepenyk
     1201 erdssdsnsl snekmnketm sdeksesktg kpeedkeeev kdelqkdqas eiedpkesst
     1261 eeadlqttts ttttttsttt ttttttvrpt ppklfkpisa rksynyippt ttpnpviikp
     1321 rlsllnpkpa kppksyndla pkpvirkfpl ltrksattms tttirgedst eipemtstev
     1381 sststevsks eenvleskld pvsqpvsepp kdhaplknkn dqdsspseqk sptnlkkeed
     1441 edftpypvar lstmatllee ilkdtestti eaentttaks ftlidveits ttaapvssts
     1501 teadhettta ksltkdeasi apstkstyqe vteasplpet stmpettstp seevesttmr
     1561 lltrkmvglr rnqggfncle kemyrfygdn rdcrlfhycs pgftsrqvld frfvceegta
     1621 fdeetqscrh nvrnkrcpdr qw
//
LOCUS       XP_034185384             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185384
VERSION     XP_034185384.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329493.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329493.2:637..2370"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185382             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185382
VERSION     XP_034185382.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329491.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329491.2:378..2111"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185388             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185388
VERSION     XP_034185388.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329497.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329497.2:372..2105"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185386             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185386
VERSION     XP_034185386.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329495.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329495.2:513..2246"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185385             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185385
VERSION     XP_034185385.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329494.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329494.2:494..2227"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185389             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185389
VERSION     XP_034185389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329498.2:547..2280"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185390             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185390
VERSION     XP_034185390.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329499.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329499.2:521..2254"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_076545792             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545792
VERSION     XP_076545792.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689677.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_076689677.1:554..2287"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185392             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185392
VERSION     XP_034185392.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329501.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329501.2:412..2145"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_076545793             575 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545793
VERSION     XP_076545793.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689678.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="uncharacterized protein LOC117606700 isoform X2"
                     /calculated_mol_wt=64268
     CDS             1..575
                     /gene="LOC117606700"
                     /coded_by="XM_076689678.1:638..2365"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fidpmtkqcp nlgryeivsv lhshagenpe eilgvegepn
      421 manaaevqdv rvtstpypgr crygsvrrld igcktpdrme fatscsdesp seywchgtwi
      481 endtaylvas tdvgryclvy sasaaatgsr elsvtghlas cprpshrhlv swqvnltsya
      541 qcgdistats wtfrisasvy vllvlgiifg ryiar
//
LOCUS       XP_034185394             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606700 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185394
VERSION     XP_034185394.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329503.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117606700 isoform X1"
                     /calculated_mol_wt=64440
     CDS             1..577
                     /gene="LOC117606700"
                     /coded_by="XM_034329503.2:258..1991"
                     /db_xref="GeneID:117606700"
ORIGIN      
        1 mkwplvltcf llphscilga shagcefpaq wnghwfqsgn qglvtvngsm mtskgrciet
       61 kddkflihdt evkcyrcivm hmkhpnvlqy ketyctiesp kpslpsmcse ltsdailisl
      121 frtgaapmpc pfkgplefty shgegecnsp lstaetctqe srlllryqac anvlssesvd
      181 veleclatwk egsthnlvar lhsarktnde dsyrcfiyeq tsnnswnlaq sadasctgli
      241 svkeaaktfk mkqknspnrc typswvvank swvaldpits rlqaspynlt ildrketrlv
      301 chsvvpihdr hyhhpfnpdk emqvqyvaka tldctngyvc lmfhrrddhv iemqlsewsq
      361 qpddvcnsst fnshstpytt fiatdpmtkq cpnlgryeiv svlhshagen peeilgvege
      421 pnmanaaevq dvrvtstpyp grcrygsvrr ldigcktpdr mefatscsde spseywchgt
      481 wiendtaylv astdvgrycl vysasaaatg srelsvtghl ascprpshrh lvswqvnlts
      541 yaqcgdista tswtfrisas vyvllvlgii fgryiar
//
LOCUS       XP_034185395             410 aa            linear   INV 03-SEP-2025
DEFINITION  quinone oxidoreductase-like protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034185395
VERSION     XP_034185395.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329504.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="quinone oxidoreductase-like protein 2"
                     /calculated_mol_wt=44963
     Region          58..406
                     /region_name="MDR"
                     /note="Medium chain reductase/dehydrogenase
                     (MDR)/zinc-dependent alcohol dehydrogenase-like family;
                     cl16912"
                     /db_xref="CDD:450120"
     Site            order(94..96,99,180,184,205..208,210..211,230..231,235,
                     250,274..275,322..323,345..347)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176178"
     CDS             1..410
                     /gene="LOC117606701"
                     /coded_by="XM_034329504.2:100..1332"
                     /db_xref="GeneID:117606701"
ORIGIN      
        1 msvvvgrril thfcknhlkk lstrtrilgf tseakeksln ledakpgipa seagkilaav
       61 lkkfnesliv enlespkmlq tnevlidvny calnasdvll sknlytfept lpmvlgyelv
      121 gklvqvgeea kkkgykigdk vialnkdryg glaeqcvaei advwkipsei ksldaiglld
      181 dyitaliale kkvslqehdi mfvnvgvsst glaavdlatn vfkaqvisvc atedgaalar
      241 ekgvlasfkf kdrtllkqie evaadkdika ifddadgkyl kkilscftdi ykpdatikdl
      301 srddsfavvl hhlsregrai iagtatiksn deskdqegsf svtglnlref rkanpetyrq
      361 agddileffe eglikptcvl nvgllkinda lefivnkklp gkvvidiknr
//
LOCUS       XP_034185396             364 aa            linear   INV 03-SEP-2025
DEFINITION  MYG1 exonuclease isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185396
VERSION     XP_034185396.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185396.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="MYG1 exonuclease isoform X1"
                     /calculated_mol_wt=41464
     Region          41..358
                     /region_name="UPF0160"
                     /note="Uncharacterized protein family (UPF0160);
                     pfam03690"
                     /db_xref="CDD:461014"
     CDS             1..364
                     /gene="LOC117606702"
                     /coded_by="XM_034329505.2:137..1231"
                     /db_xref="GeneID:117606702"
ORIGIN      
        1 mvrgisktvl qyfsnaitsp ghltytkvks ryitimteni kigthdgcfh cdealacfml
       61 ktlpqykdai iirsrdkgil dtcdivvdvg geynpskhry dhhmrdfkes vstvikkpgy
      121 dwdiklssag liychfgyei ikhlfpqlsd nvveiifkri ydklikeidg idngipmyke
      181 eplyrivtdl ssrvdflnvk wndkdtnvda rfvkameltg qdflqhinya vnvwlpakai
      241 vqeaiakrfe vdpsgeiivl sqcvpwaehi feiekemnvq pplkylifkd nnyrircvpv
      301 kpgsfvtrlf lpeawaglrg eeleracgip gaefvhsvrf igghstlega vtmarkslel
      361 gkki
//
LOCUS       XP_034185398             329 aa            linear   INV 03-SEP-2025
DEFINITION  MYG1 exonuclease isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185398
VERSION     XP_034185398.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329507.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185398.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="MYG1 exonuclease isoform X2"
                     /calculated_mol_wt=37508
     Region          6..323
                     /region_name="UPF0160"
                     /note="Uncharacterized protein family (UPF0160);
                     pfam03690"
                     /db_xref="CDD:461014"
     CDS             1..329
                     /gene="LOC117606702"
                     /coded_by="XM_034329507.2:169..1158"
                     /db_xref="GeneID:117606702"
ORIGIN      
        1 mtenikigth dgcfhcdeal acfmlktlpq ykdaiiirsr dkgildtcdi vvdvggeynp
       61 skhrydhhmr dfkesvstvi kkpgydwdik lssagliych fgyeiikhlf pqlsdnvvei
      121 ifkriydkli keidgidngi pmykeeplyr ivtdlssrvd flnvkwndkd tnvdarfvka
      181 meltgqdflq hinyavnvwl pakaivqeai akrfevdpsg eiivlsqcvp waehifeiek
      241 emnvqpplky lifkdnnyri rcvpvkpgsf vtrlflpeaw aglrgeeler acgipgaefv
      301 hsvrfigghs tlegavtmar kslelgkki
//
LOCUS       XP_034185399             329 aa            linear   INV 03-SEP-2025
DEFINITION  MYG1 exonuclease isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185399
VERSION     XP_034185399.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329508.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185399.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="MYG1 exonuclease isoform X2"
                     /calculated_mol_wt=37508
     Region          6..323
                     /region_name="UPF0160"
                     /note="Uncharacterized protein family (UPF0160);
                     pfam03690"
                     /db_xref="CDD:461014"
     CDS             1..329
                     /gene="LOC117606702"
                     /coded_by="XM_034329508.2:173..1162"
                     /db_xref="GeneID:117606702"
ORIGIN      
        1 mtenikigth dgcfhcdeal acfmlktlpq ykdaiiirsr dkgildtcdi vvdvggeynp
       61 skhrydhhmr dfkesvstvi kkpgydwdik lssagliych fgyeiikhlf pqlsdnvvei
      121 ifkriydkli keidgidngi pmykeeplyr ivtdlssrvd flnvkwndkd tnvdarfvka
      181 meltgqdflq hinyavnvwl pakaivqeai akrfevdpsg eiivlsqcvp waehifeiek
      241 emnvqpplky lifkdnnyri rcvpvkpgsf vtrlflpeaw aglrgeeler acgipgaefv
      301 hsvrfigghs tlegavtmar kslelgkki
//
LOCUS       XP_034185400             327 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606703 [Osmia lignaria lignaria].
ACCESSION   XP_034185400
VERSION     XP_034185400.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329509.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="uncharacterized protein LOC117606703"
                     /calculated_mol_wt=37160
     Region          12..133
                     /region_name="DUF4743"
                     /note="Domain of unknown function (DUF4743); pfam15916"
                     /db_xref="CDD:406365"
     Region          128..279
                     /region_name="NUDIX_Tnr3_like"
                     /note="thiamine diphosphokinase Tnr3 from
                     Schizosaccharomyces pombe and similar proteins; cd03676"
                     /db_xref="CDD:467544"
     CDS             1..327
                     /gene="LOC117606703"
                     /coded_by="XM_034329509.2:284..1267"
                     /db_xref="GeneID:117606703"
ORIGIN      
        1 mtdsdkkpms rllklankfn cfylsgfhag ecrafvvdgq qvglvrpdvm kellnhpqvf
       61 qvhpeyvqln pafrdysers arveevlrew ragekfvtlr gwreecyevr aqfntpplfk
      121 mdrsatclfg irkygvding yvmdpvkgls iwlqkrspnk qtwpgywdnm vsgglsvgyg
      181 inetaikeag eeasipnnli aklksagcvs lffeserglf pntefvydle lppdfvpsnn
      241 dgevetfell pvnecleril sshfkttsvp valdflirhg yitaenepnf ieivellhvp
      301 lqtmynqsqk kckissngea iesleri
//
LOCUS       XP_034185401             327 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606703 [Osmia lignaria lignaria].
ACCESSION   XP_034185401
VERSION     XP_034185401.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329510.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="uncharacterized protein LOC117606703"
                     /calculated_mol_wt=37160
     Region          12..133
                     /region_name="DUF4743"
                     /note="Domain of unknown function (DUF4743); pfam15916"
                     /db_xref="CDD:406365"
     Region          128..279
                     /region_name="NUDIX_Tnr3_like"
                     /note="thiamine diphosphokinase Tnr3 from
                     Schizosaccharomyces pombe and similar proteins; cd03676"
                     /db_xref="CDD:467544"
     CDS             1..327
                     /gene="LOC117606703"
                     /coded_by="XM_034329510.2:164..1147"
                     /db_xref="GeneID:117606703"
ORIGIN      
        1 mtdsdkkpms rllklankfn cfylsgfhag ecrafvvdgq qvglvrpdvm kellnhpqvf
       61 qvhpeyvqln pafrdysers arveevlrew ragekfvtlr gwreecyevr aqfntpplfk
      121 mdrsatclfg irkygvding yvmdpvkgls iwlqkrspnk qtwpgywdnm vsgglsvgyg
      181 inetaikeag eeasipnnli aklksagcvs lffeserglf pntefvydle lppdfvpsnn
      241 dgevetfell pvnecleril sshfkttsvp valdflirhg yitaenepnf ieivellhvp
      301 lqtmynqsqk kckissngea iesleri
//
LOCUS       XP_034185403             321 aa            linear   INV 03-SEP-2025
DEFINITION  putative rRNA-processing protein EBP2 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034185403
VERSION     XP_034185403.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329512.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="putative rRNA-processing protein EBP2 homolog"
                     /calculated_mol_wt=36952
     Region          56..312
                     /region_name="Ebp2"
                     /note="Eukaryotic rRNA processing protein EBP2; pfam05890"
                     /db_xref="CDD:461770"
     CDS             1..321
                     /gene="LOC117606704"
                     /coded_by="XM_034329512.2:148..1113"
                     /db_xref="GeneID:117606704"
ORIGIN      
        1 mkvknasnnv eeasessesd seynssdeel reayakgllk pglnvvveqt kkqhkncvnl
       61 mkekleaikl klpwverldl tnapaplape lalqmqeqev rrakqlkgnk kipqydpaed
      121 pvlndfrret mfhrqaqgav mdgiarlkkl gipttrpedy faemaksdth mqkvrqnlmk
      181 kqtitqrsek irqlrqqrkv gkqmqieatl kkhaekrklm eevkkyrkgm rqdldfldek
      241 kkpqkkpinp riaakrkmkd vkfgfggkkr gskkntrnss advseykrpa khgkdlkkkg
      301 ggkvkqrlgk grrvqmkakr k
//
LOCUS       XP_034185404             277 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606705 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185404
VERSION     XP_034185404.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329513.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..277
                     /product="uncharacterized protein LOC117606705 isoform X1"
                     /calculated_mol_wt=28046
     CDS             1..277
                     /gene="LOC117606705"
                     /coded_by="XM_034329513.2:81..914"
                     /db_xref="GeneID:117606705"
ORIGIN      
        1 mgpvvlllag lisgalakpg ilsgqliatl tpgasigadg rvldtqevav araehaaahi
       61 nerlkladea arssdgnlal vkqsslllas prilvpgapi gpdgrvldtp evavaraaha
      121 aaqvnerinl aneaarsaaa dlnrhtfrqv vpvvtngili rgatigpdgr vqdtqevaaa
      181 raahaaaqin erinlaneav rsgaivtgps lavplvvpra pigpdgrvqd tpevavakaa
      241 hatahlnekl nlatesaksa dvlavagpaf aygrlvy
//
LOCUS       XP_034185405             273 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606705 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185405
VERSION     XP_034185405.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329514.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="uncharacterized protein LOC117606705 isoform X2"
                     /calculated_mol_wt=27504
     CDS             1..273
                     /gene="LOC117606705"
                     /coded_by="XM_034329514.2:81..902"
                     /db_xref="GeneID:117606705"
ORIGIN      
        1 mgpvvlllag lisgalakpg ilsgqliatl tpgasigadg rvldtqevav araehaaahi
       61 nerlkladea arssdgnlal vkqsslllas prilvpgapi gpdgrvldtp evavaraaha
      121 aaqvnerinl aneaarsaaa dlnrqvvpvv tngilirgat igpdgrvqdt qevaaaraah
      181 aaaqinerin laneavrsga ivtgpslavp lvvprapigp dgrvqdtpev avakaahata
      241 hlneklnlat esaksadvla vagpafaygr lvy
//
LOCUS       XP_034185212             274 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606623 [Osmia lignaria lignaria].
ACCESSION   XP_034185212
VERSION     XP_034185212.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329321.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185212.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..274
                     /product="uncharacterized protein LOC117606623"
                     /calculated_mol_wt=29083
     Region          <82..>252
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     CDS             1..274
                     /gene="LOC117606623"
                     /coded_by="XM_034329321.2:143..967"
                     /db_xref="GeneID:117606623"
ORIGIN      
        1 mkglvilval vasckagfld apsyhepqir pqavpqrfap papvgqdgnv idtpevaeak
       61 aahfaefara aaraaeqlkn qpqnaqeynp rgssqsynyp saqptaatyv rqpsyqssap
      121 iyqsapvpap mytqpnpvay qpqytgntny vgqqnyappv kptpfvpapl addgtvidtp
      181 evaalkaarl aelaeaeara ykfaqdfkpe aqgqvyggpp agpspvqyna gqynapagrv
      241 ypasgpsytg pqasfskpaf qpqnyqqqql vgay
//
LOCUS       XP_076545794             167 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305573 [Osmia lignaria lignaria].
ACCESSION   XP_076545794
VERSION     XP_076545794.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689679.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..167
                     /product="uncharacterized protein LOC143305573"
                     /calculated_mol_wt=17267
     CDS             1..167
                     /gene="LOC143305573"
                     /coded_by="XM_076689679.1:148..651"
                     /db_xref="GeneID:143305573"
ORIGIN      
        1 mntliifsti lavalarpgl llspqvatlt spvaiarivh gapigldgrv vdtpevayak
       61 aehaaahine ritlaneaik sadviaytsp vitsnvepit iaklvpsapv gpdgrvvdtp
      121 evayakaeha aahinekigh iaeagksgdf pvvistyssp liqkyil
//
LOCUS       XP_034185211             166 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606622 [Osmia lignaria lignaria].
ACCESSION   XP_034185211
VERSION     XP_034185211.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329320.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185211.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="uncharacterized protein LOC117606622"
                     /calculated_mol_wt=16976
     CDS             1..166
                     /gene="LOC117606622"
                     /coded_by="XM_034329320.2:44..544"
                     /db_xref="GeneID:117606622"
ORIGIN      
        1 mirylvllfv tlhvvccapq wyggghpgay gghaapaplg pdgrvvdtpe vaqlkaahla
       61 aladanarap kglgpagpyp gpagsyapgn yaahysgppa plgpdgrvvd tpevqqakav
      121 hfslysaeaq raptgpagpa apvphnpswn sgaynpswne nswnqy
//
LOCUS       XP_076545795             141 aa            linear   INV 03-SEP-2025
DEFINITION  cuticular protein 92F [Osmia lignaria lignaria].
ACCESSION   XP_076545795
VERSION     XP_076545795.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689680.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..141
                     /product="cuticular protein 92F"
                     /calculated_mol_wt=14917
     CDS             1..141
                     /gene="Cpr92F"
                     /coded_by="XM_076689680.1:490..915"
                     /db_xref="GeneID:143305574"
ORIGIN      
        1 mrlllpfscf llsvtaipty gtypvggiyh gppaplaqdg rvidtpevah akaahlaaha
       61 ieaakasslg ysdypdgkye eyvspaqnvy hgppaplahd grvidtpeva hakaahlaah
      121 adqlskivkq sygdaypqvh w
//
LOCUS       XP_034184897            1968 aa            linear   INV 03-SEP-2025
DEFINITION  endoribonuclease Dcr-1 [Osmia lignaria lignaria].
ACCESSION   XP_034184897
VERSION     XP_034184897.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329006.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184897.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1968
                     /product="endoribonuclease Dcr-1"
                     /calculated_mol_wt=226828
     Region          14..205
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(38..44,141)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     Region          236..340
                     /region_name="Dicer_PBD"
                     /note="Partner-binding domain of the endoribonuclease
                     Dicer; cd15903"
                     /db_xref="CDD:277191"
     Site            order(241..242,245,247..248,318..319,322..323,326,
                     329..330,333)
                     /site_type="other"
                     /note="Trbp binding interface [polypeptide binding]"
                     /db_xref="CDD:277191"
     Region          428..554
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            542
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350171"
     Region          619..711
                     /region_name="Dicer_dimer"
                     /note="Dicer dimerization domain; pfam03368"
                     /db_xref="CDD:460900"
     Region          884..1003
                     /region_name="PAZ_dicer_like"
                     /note="PAZ domain, dicer_like subfamily. Dicer is an RNAse
                     involved in cleaving dsRNA in the RNA interference
                     pathway. It generates dsRNAs which are approximately 20 bp
                     long (siRNAs), which in turn target hydrolysis of
                     homologous RNAs. PAZ domains are named...; cd02843"
                     /db_xref="CDD:239209"
     Site            order(944,957,963,967,985,993,995)
                     /site_type="other"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]"
                     /db_xref="CDD:239209"
     Region          1417..>1507
                     /region_name="RIBOc"
                     /note="Ribonuclease III family; smart00535"
                     /db_xref="CDD:197778"
     Site            order(1435..1436,1439..1440,1443,1446..1447,1450..1451,
                     1453..1455,1465)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238333"
     Region          1695..1941
                     /region_name="Rnc"
                     /note="dsRNA-specific ribonuclease [Transcription];
                     COG0571"
                     /db_xref="CDD:440336"
     CDS             1..1968
                     /gene="Dcr-1"
                     /coded_by="XM_034329006.2:297..6203"
                     /db_xref="GeneID:117606504"
ORIGIN      
        1 mafplndqiy tksftpreyq velfyaarnk niivclgkty eqtfivikli qefaannrrl
       61 lseggkrsly iltdeekcti kssyiqqltd lkvllcstcs sgefeetfet shvlvltset
      121 ctellaekki lphqinlviv dechkfindk klkfilqtfl scsnvpriig lavplfnltq
      181 eagrlgleie kveatfkcev etasdilsil ryspkakeyv veysngekge lhstirnyil
      241 haieflrdhr hdpteiynee fyediqkipd ptekpfemmq dflhiletlg pwcadraala
      301 llilteklki ktpyerhyll lnmvasvfik iralcdnafe alsekervyk yttpkvhrll
      361 qilktytpyy tkdnnnshnk ingenssqrl tvnnrensir dkfnqtkkan eenhkkpfkf
      421 qrhmrgatdp dllcgvifvd kgfvakvlfy llneisahde dleflsplyt ierntddvsy
      481 skdleiehkk qeevlkkfri hecnllists ileegidipk cnfvmrydfp ktyqsyvqck
      541 sraraidalh vllvpketsk eciwqlaqyh yiektllikc snnepseeee nvadmyaami
      601 phykplsgdd apkvtfnsai slvnrycakl psdtftrltp ewsiepvivd nmpmyicslr
      661 lpinspvkya vssypmpnra marrmaalql cidlhrknei ddnllpigke nfkakpedae
      721 vpalpdeiki dfsearpgtt krrqyyykkt analtdcrpi vgvpsylyhi nmvlscplpe
      781 eqntrgrriy ppeesaigfg iitlkeipkl cpfpiytrsg evhvklklsk nivilnetqi
      841 ekiatflnyt ftnvlrlqqy lmlfdpnase nsymivpvkl dtdsdvtvdw efleciynnr
      901 ttvptkvpee erkdfkfdal kyhdavimpw yrnqdqpqyf yvaeictnln pkssfpghdy
      961 stfeeyyfkk ygiqiqnldq plldvdhtsa rlnfltpryv nrkgvalpts seetkrakre
     1021 nleqkqilva elcaihpfpa slwrqavclp cilyrinall lanqircqva qminlgqenl
     1081 asdfewpald fgwslaevlk kskesekakm akndttqlns aintidnkyq nesekkinnl
     1141 nkldmedlie keyefqmend nmtekeleis ndedeleigt wsndmamnti dcetndlksf
     1201 pvtvlpnnfn wsdvrygspa cesdydgyvs ddiysdgfvd ssdesedenr glrisymgen
     1261 vaeavedekq iskqeinkki ldlleteknl dtlwsytdeq eesliakhrq thyeftkire
     1321 ceimengsfi scdkeivikr knsfnsqled nssdhkykvh vqsivnlftt evldnyntlq
     1381 likqksnnsk ftyhpsnnlf sfdfqpelen hpgpspslil qaltmsnand ginlerleti
     1441 gdsflkyait tylyctydni hegklshlrs kqvsnlnlyr lgrqkmlges miatkfephd
     1501 nwlppcyyvp keleqalies gipstlwnqa diptlqavnp neitqlvret eqklgimkde
     1561 lvrneamlsn sldnlrcfip ynlitqhsip dksiadcvea ligayliacg prgallfmaw
     1621 lgihvlpvee vgivqetepe eripgstpyv kgtnekgett waqirygkle epqnpllryi
     1681 pdpeqelqmm ldgyeeleks igykfndisy llqafthasy qpnkltdcyq rleflgdavl
     1741 dylitrhlye darqhspgal tdlrsalvnn tifaslavrc gfhkyfrhls pglsvvinrf
     1801 vriqeenghs iseeyylige ekceeaedve vpkalgdvfe slagaiylds gmsldavwsv
     1861 yyvimkneie qfstnvpksp irellelepe takfgkpekl adgrrvrvtv dvfgkgsfkg
     1921 igrnyriakc taakcalkkl kqmqnyprdk driselkhek srerlfkc
//
LOCUS       XP_034185600            1642 aa            linear   INV 03-SEP-2025
DEFINITION  NHS actin remodeling regulator GUK-holder isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185600
VERSION     XP_034185600.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329709.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185600.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1642
                     /product="NHS actin remodeling regulator GUK-holder
                     isoform X1"
                     /calculated_mol_wt=179151
     CDS             1..1642
                     /gene="gukh"
                     /coded_by="XM_034329709.2:405..5333"
                     /db_xref="GeneID:117606799"
ORIGIN      
        1 mpfvlrrvep rflcrghvpa dptpqgwpvg aeleavanga ltaslkqlas lltiaedifa
       61 eltaelttva drssnlrhrl dkveerlagv dpkkipvpes dictfaarke nfhmvnkpst
      121 slftlntrpk alrelyeaaa ivavrsldsl rrdgssmdmf lctpvlgkkk rdqsldiesr
      181 ipaviedlrr wtstealgdv tvppdcmsri lgdtttddav dhklpspeeq vqaialkfpa
      241 eivavdvsgr gfermsmrrr sllsgpesqe tavkrrsrpr rprgkrrnti agtdqkeirq
      301 aiggetigee aeetmsnits rahrsastdi lgsskkespn ekkshfntlk awgmsrlkli
      361 spksspqqdq qestcstter seqyegqsrk aeetniyerv tprrrkdksh erkpssssss
      421 gkstssipvs vpsterqpsv klresaaqrr errkgssrde pphsssgnws assesgrasi
      481 gsettttthq prsttttsvg tsstslshvr rfkrrdtsts ssvtsegtlt pdiiqditvv
      541 pfsddgetss vyscdtegyy tsfhvdsglk tlreedpvpq spltksndvs sdptspiven
      601 eyelfgrgst stttssagtv ctalmapppp erkssltvva mvhgpnqngg espdsghnts
      661 sspvesassp actgrscsef eysessdleg cerierirsk taintsrips mcvitppgsd
      721 dehaidldqt skknenrkvd lqaagsvlms atvgtskmev ingqdknlya tiqvlpsptn
      781 ekavsgqast kisplsgvlg klrgvlpsrk hmpksavqdq pnadsgdyvt iadvrnnndk
      841 rqdstatpvq ssvtyansdi fkkdaeyvsl selpkkpdss lerkrqgarv tldsegkvvy
      901 ssdslkrrkg ahttfnpgpf vregvspnps pllhratpnv ravtkssdav dgptpkstpp
      961 tspqlgkvii raarsperaa spdyvrtpsm vvgptspttp hrqvrgayvn vqddedntll
     1021 aidsapphgi vqpppyispp kpegcqkdhm iqdilkeppa sqphcekqqn ygptqkiyth
     1081 dprqsfydnn nqlsnyndhr qiiqmrnarn qqyyvsnkdg nhqfytlpsr rpqrdveppr
     1141 svtpditrgl rrgslsamhm larhgqktil eqdsksygsq velsnrtsen vetenrlmqg
     1201 qqqppivdvr nrfatplglt algyrffass pirdpvmksp sadalkhnpi spirhtcdns
     1261 fksstptgrt mptvaerlal tannnhpssm pnsgrstpvq tssgrstptn lilsptkstm
     1321 sneelfaaih kskkrlnikd dsenqspygs tnslakmapg srsswsseaq kpqaaapnas
     1381 spspatsrld fkrlllqqsv ktgptrlsaa eqlklsrqqc qqqqssptip qttplakvls
     1441 prsvwrfqtp rtdvlsstii edtaaeekam kpspenispv srlsarrqld lcsdlpdylr
     1501 iskenntrvp sasaeasdsk pgehvrttne srlvvsaagq nvsvqsvpvs ysqnpttspt
     1561 vqysanspql ttscqqaisa fesrrisnql araqflastp ttnqtqntcv kkvfrarsvs
     1621 pqnpgsqnva rspsaptlet al
//
LOCUS       XP_034185601            1619 aa            linear   INV 03-SEP-2025
DEFINITION  NHS actin remodeling regulator GUK-holder isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185601
VERSION     XP_034185601.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329710.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185601.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1619
                     /product="NHS actin remodeling regulator GUK-holder
                     isoform X2"
                     /calculated_mol_wt=176398
     CDS             1..1619
                     /gene="gukh"
                     /coded_by="XM_034329710.2:405..5264"
                     /db_xref="GeneID:117606799"
ORIGIN      
        1 mpfvlrrvep rflcrghvpa dptpqgwpvg aeleavanga ltaslkqlas lltiaedifa
       61 eltaelttva drssnlrhrl dkveerlagv dpkkipvpes dictfaarke nfhmvnkpst
      121 slftlntrpk alrelyeaaa ivavrsldsl rrdgssmdmf lctpvlgkkk rdqsldiesr
      181 ipaviedlrr wtstealgdv tvppdcmsri lgdtttddav dhklpspeeq vqaialkfpa
      241 eivavdvsgr gfermsmrrr sllsgpesqe tavkrrsrpr rprgkrrnti agtdqkeirq
      301 aiggetigee aeetmsnits rahrsastdi lgsskkespn ekkshfntlk awgmsrlkli
      361 spksspqqdq qestcstter rrkdksherk pssssssgks tssipvsvps terqpsvklr
      421 esaaqrrerr kgssrdepph sssgnwsass esgrasigse ttttthqprs ttttsvgtss
      481 tslshvrrfk rrdtstsssv tsegtltpdi iqditvvpfs ddgetssvys cdtegyytsf
      541 hvdsglktlr eedpvpqspl tksndvssdp tspiveneye lfgrgststt tssagtvcta
      601 lmapppperk ssltvvamvh gpnqnggesp dsghntsssp vesasspact grscsefeys
      661 essdlegcer ierirsktai ntsripsmcv itppgsddeh aidldqtskk nenrkvdlqa
      721 agsvlmsatv gtskmeving qdknlyatiq vlpsptneka vsgqastkis plsgvlgklr
      781 gvlpsrkhmp ksavqdqpna dsgdyvtiad vrnnndkrqd statpvqssv tyansdifkk
      841 daeyvslsel pkkpdssler krqgarvtld segkvvyssd slkrrkgaht tfnpgpfvre
      901 gvspnpspll hratpnvrav tkssdavdgp tpkstpptsp qlgkviiraa rsperaaspd
      961 yvrtpsmvvg ptspttphrq vrgayvnvqd dedntllaid sapphgivqp ppyisppkpe
     1021 gcqkdhmiqd ilkeppasqp hcekqqnygp tqkiythdpr qsfydnnnql snyndhrqii
     1081 qmrnarnqqy yvsnkdgnhq fytlpsrrpq rdvepprsvt pditrglrrg slsamhmlar
     1141 hgqktileqd sksygsqvel snrtsenvet enrlmqgqqq ppivdvrnrf atplgltalg
     1201 yrffasspir dpvmkspsad alkhnpispi rhtcdnsfks stptgrtmpt vaerlaltan
     1261 nnhpssmpns grstpvqtss grstptnlil sptkstmsne elfaaihksk krlnikddse
     1321 nqspygstns lakmapgsrs swsseaqkpq aaapnassps patsrldfkr lllqqsvktg
     1381 ptrlsaaeql klsrqqcqqq qssptipqtt plakvlsprs vwrfqtprtd vlsstiiedt
     1441 aaeekamkps penispvsrl sarrqldlcs dlpdylrisk enntrvpsas aeasdskpge
     1501 hvrttnesrl vvsaagqnvs vqsvpvsysq npttsptvqy sanspqltts cqqaisafes
     1561 rrisnqlara qflastpttn qtqntcvkkv frarsvspqn pgsqnvarsp saptletal
//
LOCUS       XP_034185602            1617 aa            linear   INV 03-SEP-2025
DEFINITION  NHS actin remodeling regulator GUK-holder isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185602
VERSION     XP_034185602.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185602.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1617
                     /product="NHS actin remodeling regulator GUK-holder
                     isoform X3"
                     /calculated_mol_wt=176086
     CDS             1..1617
                     /gene="gukh"
                     /coded_by="XM_034329711.2:405..5258"
                     /db_xref="GeneID:117606799"
ORIGIN      
        1 mpfvlrrvep rflcrghvpa dptpqgwpvg aeleavanga ltaslkqlas lltiaedifa
       61 eltaelttva drssnlrhrl dkveerlagv dpkkipvpes dictfaarke nfhmvnkpst
      121 slftlntrpk alrelyeaaa ivavrsldsl rrdgssmdmf lctpvlgkkk rdqsldiesr
      181 ipaviedlrr wtstealgdv tvppdcmsri lgdtttddav dhklpspeeq vqaialkfpa
      241 eivavdvsgr gfermsmrrr sllsgpesqe tavkrrsrpr rprgkrrnti agtdqkeirq
      301 aiggetigee aeetmsnits rahrsastdi lgsskkespn ekkshfntlk awgmsrlkli
      361 spksspqqdq qestcstter kdksherkps sssssgksts sipvsvpste rqpsvklres
      421 aaqrrerrkg ssrdepphss sgnwsasses grasigsett ttthqprstt ttsvgtssts
      481 lshvrrfkrr dtstsssvts egtltpdiiq ditvvpfsdd getssvyscd tegyytsfhv
      541 dsglktlree dpvpqspltk sndvssdpts piveneyelf grgststtts sagtvctalm
      601 apppperkss ltvvamvhgp nqnggespds ghntssspve sasspactgr scsefeyses
      661 sdlegcerie rirsktaint sripsmcvit ppgsddehai dldqtskkne nrkvdlqaag
      721 svlmsatvgt skmevingqd knlyatiqvl psptnekavs gqastkispl sgvlgklrgv
      781 lpsrkhmpks avqdqpnads gdyvtiadvr nnndkrqdst atpvqssvty ansdifkkda
      841 eyvslselpk kpdsslerkr qgarvtldse gkvvyssdsl krrkgahttf npgpfvregv
      901 spnpspllhr atpnvravtk ssdavdgptp kstpptspql gkviiraars peraaspdyv
      961 rtpsmvvgpt spttphrqvr gayvnvqdde dntllaidsa pphgivqppp yisppkpegc
     1021 qkdhmiqdil keppasqphc ekqqnygptq kiythdprqs fydnnnqlsn yndhrqiiqm
     1081 rnarnqqyyv snkdgnhqfy tlpsrrpqrd vepprsvtpd itrglrrgsl samhmlarhg
     1141 qktileqdsk sygsqvelsn rtsenveten rlmqgqqqpp ivdvrnrfat plgltalgyr
     1201 ffasspirdp vmkspsadal khnpispirh tcdnsfksst ptgrtmptva erlaltannn
     1261 hpssmpnsgr stpvqtssgr stptnlilsp tkstmsneel faaihkskkr lnikddsenq
     1321 spygstnsla kmapgsrssw sseaqkpqaa apnasspspa tsrldfkrll lqqsvktgpt
     1381 rlsaaeqlkl srqqcqqqqs sptipqttpl akvlsprsvw rfqtprtdvl sstiiedtaa
     1441 eekamkpspe nispvsrlsa rrqldlcsdl pdylrisken ntrvpsasae asdskpgehv
     1501 rttnesrlvv saagqnvsvq svpvsysqnp ttsptvqysa nspqlttscq qaisafesrr
     1561 isnqlaraqf lastpttnqt qntcvkkvfr arsvspqnpg sqnvarspsa ptletal
//
LOCUS       XP_076545796            1462 aa            linear   INV 03-SEP-2025
DEFINITION  NHS actin remodeling regulator GUK-holder isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545796
VERSION     XP_076545796.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689681.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1462
                     /product="NHS actin remodeling regulator GUK-holder
                     isoform X4"
                     /calculated_mol_wt=159247
     CDS             1..1462
                     /gene="gukh"
                     /coded_by="XM_076689681.1:819..5207"
                     /db_xref="GeneID:117606799"
ORIGIN      
        1 mpaviedlrr wtstealgdv tvppdcmsri lgdtttddav dhklpspeeq vqaialkfpa
       61 eivavdvsgr gfermsmrrr sllsgpesqe tavkrrsrpr rprgkrrnti agtdqkeirq
      121 aiggetigee aeetmsnits rahrsastdi lgsskkespn ekkshfntlk awgmsrlkli
      181 spksspqqdq qestcstter seqyegqsrk aeetniyerv tprrrkdksh erkpssssss
      241 gkstssipvs vpsterqpsv klresaaqrr errkgssrde pphsssgnws assesgrasi
      301 gsettttthq prsttttsvg tsstslshvr rfkrrdtsts ssvtsegtlt pdiiqditvv
      361 pfsddgetss vyscdtegyy tsfhvdsglk tlreedpvpq spltksndvs sdptspiven
      421 eyelfgrgst stttssagtv ctalmapppp erkssltvva mvhgpnqngg espdsghnts
      481 sspvesassp actgrscsef eysessdleg cerierirsk taintsrips mcvitppgsd
      541 dehaidldqt skknenrkvd lqaagsvlms atvgtskmev ingqdknlya tiqvlpsptn
      601 ekavsgqast kisplsgvlg klrgvlpsrk hmpksavqdq pnadsgdyvt iadvrnnndk
      661 rqdstatpvq ssvtyansdi fkkdaeyvsl selpkkpdss lerkrqgarv tldsegkvvy
      721 ssdslkrrkg ahttfnpgpf vregvspnps pllhratpnv ravtkssdav dgptpkstpp
      781 tspqlgkvii raarsperaa spdyvrtpsm vvgptspttp hrqvrgayvn vqddedntll
      841 aidsapphgi vqpppyispp kpegcqkdhm iqdilkeppa sqphcekqqn ygptqkiyth
      901 dprqsfydnn nqlsnyndhr qiiqmrnarn qqyyvsnkdg nhqfytlpsr rpqrdveppr
      961 svtpditrgl rrgslsamhm larhgqktil eqdsksygsq velsnrtsen vetenrlmqg
     1021 qqqppivdvr nrfatplglt algyrffass pirdpvmksp sadalkhnpi spirhtcdns
     1081 fksstptgrt mptvaerlal tannnhpssm pnsgrstpvq tssgrstptn lilsptkstm
     1141 sneelfaaih kskkrlnikd dsenqspygs tnslakmapg srsswsseaq kpqaaapnas
     1201 spspatsrld fkrlllqqsv ktgptrlsaa eqlklsrqqc qqqqssptip qttplakvls
     1261 prsvwrfqtp rtdvlsstii edtaaeekam kpspenispv srlsarrqld lcsdlpdylr
     1321 iskenntrvp sasaeasdsk pgehvrttne srlvvsaagq nvsvqsvpvs ysqnpttspt
     1381 vqysanspql ttscqqaisa fesrrisnql araqflastp ttnqtqntcv kkvfrarsvs
     1441 pqnpgsqnva rspsaptlet al
//
LOCUS       XP_034184944            1164 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase hopscotch isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184944
VERSION     XP_034184944.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329053.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184944.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1164
                     /product="tyrosine-protein kinase hopscotch isoform X1"
                     /calculated_mol_wt=132880
     Region          13..192
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          201..303
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          303..398
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(370,396)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     Region          485..713
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          884..1157
                     /region_name="TyrKc"
                     /note="Tyrosine kinase, catalytic domain; smart00219"
                     /db_xref="CDD:197581"
     Site            order(890..894,898,917,919,964..967,970..971,1011,
                     1015..1016,1018,1029,1047..1051,1060,1095)
                     /site_type="active"
                     /db_xref="CDD:270623"
     Site            order(890..891,893..894,898,917,919,964..967,970..971,
                     1015..1016,1018,1029)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270623"
     Site            order(1011,1015,1047..1051,1060,1095)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270623"
     Site            order(1028..1035,1047..1053)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270623"
     CDS             1..1164
                     /gene="hop"
                     /coded_by="XM_034329053.2:173..3667"
                     /db_xref="GeneID:117606520"
ORIGIN      
        1 mesdrhaiiy vaneekelty siaygqtied lsikickslg igpvarhlfa vrnhytklwy
       61 slahrleakd knkfdfrlrf kpsslyrlkq idenaydyyf qqaradvmdn kvpdivyerh
      121 kqeliglgvs dmyrvmlekk vpretvesey rkyipkecik rhaffikkpi hnalrkisgh
      181 danyvkeqyl kqfqcmapny pyeeyealmd rgfqnppakv slrvnvnevk yyeanatswt
      241 tlcaiedlcf vsirqdntve isrkngipsy lkfskstflm sfvsaldgyy rlavkwtfnl
      301 cgeivtpsle rlyklkchgp vgqdfsyakl lekrankpgc yllrecgtey dtyyldgynk
      361 egklfskkie ektlsnefti vgspnrynsl aqcvasfqds egqsyltecl ppseydkspl
      421 llcapesavs evaadeeiva svletgprcv pqnqleiykp flkitrnhhy sptvlhraiw
      481 rltkgkklev alkilkqeee snytkeflel agkwsqlrsg tlvrlygltv tpsigmllel
      541 vrhgtldeyl rssssqtvkt vdmveatacl atalwhleen gvvhgnikcn kllvhahtnn
      601 sfvvkladpg lfvyketdih wlppetytnp elarysfqad vwavgttiwq ifargatlle
      661 lqnvvvmkkf yesgkrlpip sgcptevyrl mrecwgdvng srkqpqaimr dinqilyqvy
      721 nsrrshayat afpklfnsel kkhspdnsdi sddnksinse srasslltdn tslpwndmdd
      781 stqslining tsvgsledls aylswssntr rdseggsvsq nnvpnmncie hstqtlrirh
      841 agdldevirr neqggeedsd srrssndslg qmrgiyeldi dcnvilqgri gqgfygevyr
      901 gtleretgkd teqqqvaikk lktkaleadi rdfereiaim ktlkhpnvve ilgvisepev
      961 clvmeyvkhg slqsyllihk qelthkrllg faldiatgme ylgsksivhr dlaarnilva
     1021 denkvkisdf glaqvtgknd yyilqtdrgl pikwyapesi rdgkfstrsd vwsfgvtmye
     1081 tfgygeeprl pgldssierl qneqekgste llaalergtr lpcpstcpqa iyiklmypcw
     1141 hlqshqrpdf atlcndirdl laqy
//
LOCUS       XP_034184945            1145 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase hopscotch isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184945
VERSION     XP_034184945.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329054.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184945.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1145
                     /product="tyrosine-protein kinase hopscotch isoform X2"
                     /calculated_mol_wt=130788
     Region          13..192
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          201..303
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          303..398
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(370,396)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     Region          476..676
                     /region_name="PK_Tyr_Ser-Thr"
                     /note="Protein tyrosine and serine/threonine kinase;
                     pfam07714"
                     /db_xref="CDD:462242"
     Region          865..1138
                     /region_name="TyrKc"
                     /note="Tyrosine kinase, catalytic domain; smart00219"
                     /db_xref="CDD:197581"
     Site            order(871..875,879,898,900,945..948,951..952,992,996..997,
                     999,1010,1028..1032,1041,1076)
                     /site_type="active"
                     /db_xref="CDD:270623"
     Site            order(871..872,874..875,879,898,900,945..948,951..952,
                     996..997,999,1010)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270623"
     Site            order(992,996,1028..1032,1041,1076)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270623"
     Site            order(1009..1016,1028..1034)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270623"
     CDS             1..1145
                     /gene="hop"
                     /coded_by="XM_034329054.2:173..3610"
                     /db_xref="GeneID:117606520"
ORIGIN      
        1 mesdrhaiiy vaneekelty siaygqtied lsikickslg igpvarhlfa vrnhytklwy
       61 slahrleakd knkfdfrlrf kpsslyrlkq idenaydyyf qqaradvmdn kvpdivyerh
      121 kqeliglgvs dmyrvmlekk vpretvesey rkyipkecik rhaffikkpi hnalrkisgh
      181 danyvkeqyl kqfqcmapny pyeeyealmd rgfqnppakv slrvnvnevk yyeanatswt
      241 tlcaiedlcf vsirqdntve isrkngipsy lkfskstflm sfvsaldgyy rlavkwtfnl
      301 cgeivtpsle rlyklkchgp vgqdfsyakl lekrankpgc yllrecgtey dtyyldgynk
      361 egklfskkie ektlsnefti vgspnrynsl aqcvasfqds egqsyltecl ppseydkspl
      421 llcapesavs evaadeeiva svletgprcv pqnqleiykp flkitrnhhy sptvlhraiw
      481 rltkgkklev alkilkqeee snytkeflel agkwsqlrsg tlvrlygltv tpsigmllel
      541 vrhgtldeyl rssssqtvkt vdmveatacl atalwhleen gvvhvkladp glfvyketdi
      601 hwlppetytn pelarysfqa dvwavgttiw qifargatll elqnvvvmkk fyesgkrlpi
      661 psgcptevyr lmrecwgdvn gsrkqpqaim rdinqilyqv ynsrrshaya tafpklfnse
      721 lkkhspdnsd isddnksins esrasslltd ntslpwndmd dstqslinin gtsvgsledl
      781 saylswssnt rrdseggsvs qnnvpnmnci ehstqtlrir hagdldevir rneqggeeds
      841 dsrrssndsl gqmrgiyeld idcnvilqgr igqgfygevy rgtleretgk dteqqqvaik
      901 klktkalead irdfereiai mktlkhpnvv eilgvisepe vclvmeyvkh gslqsyllih
      961 kqelthkrll gfaldiatgm eylgsksivh rdlaarnilv adenkvkisd fglaqvtgkn
     1021 dyyilqtdrg lpikwyapes irdgkfstrs dvwsfgvtmy etfgygeepr lpgldssier
     1081 lqneqekgst ellaalergt rlpcpstcpq aiyiklmypc whlqshqrpd fatlcndird
     1141 llaqy
//
LOCUS       XP_034184947             132 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S30 [Osmia lignaria lignaria].
ACCESSION   XP_034184947
VERSION     XP_034184947.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329056.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..132
                     /product="ribosomal protein S30"
                     /calculated_mol_wt=14471
     Region          1..71
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          72..129
                     /region_name="Ribosomal_S30"
                     /note="Ribosomal protein S30; pfam04758"
                     /db_xref="CDD:398432"
     CDS             1..132
                     /gene="RpS30"
                     /coded_by="XM_034329056.2:4..402"
                     /db_xref="GeneID:117606521"
ORIGIN      
        1 mqlqirgeht tvvdshenet igelkkrive lnaaantgfn lycsgfllqn davigeltsn
       61 vleltvslpg gkvhgslara gkvkaqtpkv ekqekskkkt grakrriqyn rrfvnvvqtf
      121 grrrgpnans ns
//
LOCUS       XP_076545797            1393 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545797
VERSION     XP_076545797.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689682.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1393
                     /product="rho GTPase-activating protein 100F isoform X1"
                     /calculated_mol_wt=151999
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1393
                     /gene="LOC117608958"
                     /coded_by="XM_076689682.1:5752..9933"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksvrkiss vasalprvtq qaehnnsqhq mqqhqqqqqq qqqvphqmqg
     1261 tlgrtglpwq qqhslhqqpp tstaavlaas trppppvkpr qvivsspgsp sseeseaspe
     1321 pinkslmggl glekgnegvt tnsagpgteq itpsssvdte egntphqeeg ekgvegdaea
     1381 sdddrhqhvp kth
//
LOCUS       XP_076545798            1393 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545798
VERSION     XP_076545798.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689683.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1393
                     /product="rho GTPase-activating protein 100F isoform X1"
                     /calculated_mol_wt=151999
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1393
                     /gene="LOC117608958"
                     /coded_by="XM_076689683.1:5752..9933"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksvrkiss vasalprvtq qaehnnsqhq mqqhqqqqqq qqqvphqmqg
     1261 tlgrtglpwq qqhslhqqpp tstaavlaas trppppvkpr qvivsspgsp sseeseaspe
     1321 pinkslmggl glekgnegvt tnsagpgteq itpsssvdte egntphqeeg ekgvegdaea
     1381 sdddrhqhvp kth
//
LOCUS       XP_076545799            1393 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545799
VERSION     XP_076545799.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689684.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1393
                     /product="rho GTPase-activating protein 100F isoform X1"
                     /calculated_mol_wt=151999
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1393
                     /gene="LOC117608958"
                     /coded_by="XM_076689684.1:5752..9933"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksvrkiss vasalprvtq qaehnnsqhq mqqhqqqqqq qqqvphqmqg
     1261 tlgrtglpwq qqhslhqqpp tstaavlaas trppppvkpr qvivsspgsp sseeseaspe
     1321 pinkslmggl glekgnegvt tnsagpgteq itpsssvdte egntphqeeg ekgvegdaea
     1381 sdddrhqhvp kth
//
LOCUS       XP_076545800            1334 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545800
VERSION     XP_076545800.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689685.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1334
                     /product="rho GTPase-activating protein 100F isoform X2"
                     /calculated_mol_wt=145410
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1334
                     /gene="LOC117608958"
                     /coded_by="XM_076689685.1:5754..9758"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksdssedf fsrfsasssy atsraqqqst pnaaasaaat aaatgaasna
     1261 gnigtnraal aaatltssta pnfnsrgsrg lhsssttgqa steqaqiags tkpltaqrrr
     1321 flhryyryyr fhsr
//
LOCUS       XP_076545801            1324 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545801
VERSION     XP_076545801.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689686.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1324
                     /product="rho GTPase-activating protein 100F isoform X3"
                     /calculated_mol_wt=143917
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1324
                     /gene="LOC117608958"
                     /coded_by="XM_076689686.1:5754..9728"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksdssedf fsrfsasssy atsraqqqst pnaaasaaat aaatgaasna
     1261 gnigtnraal aaatltssta pnfnsrgsrg lhsssttgqa steqaqiags tkpltaqrrr
     1321 flhr
//
LOCUS       XP_076545802            1312 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545802
VERSION     XP_076545802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689687.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1312
                     /product="rho GTPase-activating protein 100F isoform X4"
                     /calculated_mol_wt=143829
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1312
                     /gene="LOC117608958"
                     /coded_by="XM_076689687.1:5754..9692"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksvrkiss vasalprvtq qaehnnsqhq mqqhqqqqqq qqqvphqmqg
     1261 tlgrtglpwq qqhslhqqpp tstaavlaas trppppvkpr qskrklpalp nr
//
LOCUS       XP_076545803            1311 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545803
VERSION     XP_076545803.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689688.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1311
                     /product="rho GTPase-activating protein 100F isoform X5"
                     /calculated_mol_wt=142334
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1311
                     /gene="LOC117608958"
                     /coded_by="XM_076689688.1:5753..9688"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksdssedf fsrfsasssy atsraqqqst pnaaasaaat aaatgaasna
     1261 gnigtnraal aaatltssta pnfnsrgsrg lhsssttgqa stgdslvsrf t
//
LOCUS       XP_076545804            1311 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545804
VERSION     XP_076545804.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689689.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1311
                     /product="rho GTPase-activating protein 100F isoform X6"
                     /calculated_mol_wt=142281
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1311
                     /gene="LOC117608958"
                     /coded_by="XM_076689689.1:5754..9689"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksdssedf fsrfsasssy atsraqqqst pnaaasaaat aaatgaasna
     1261 gnigtnraal aaatltssta pnfnsrgsrg lhsssttgqa stdvplsslt p
//
LOCUS       XP_076545805            1307 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase-activating protein 100F isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545805
VERSION     XP_076545805.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689690.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1307
                     /product="rho GTPase-activating protein 100F isoform X7"
                     /calculated_mol_wt=143350
     Region          199..281
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(210..216,230,233,263,267,270..271)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     Region          851..959
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     Region          1005..1211
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1060,1100,1104,1174,1177..1178,1200)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1060
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1307
                     /gene="LOC117608958"
                     /coded_by="XM_076689690.1:5754..9677"
                     /db_xref="GeneID:117608958"
ORIGIN      
        1 mdtgtgtklt siglrtdgei dkrkeerswt qetalergae gskgeelaag fsedtsnkrg
       61 cksaaqvwms llslcdrmcg stawlvlftc lhgegrgevt disaspgrqa panqlrpkep
      121 ppmviqgdfr kvsgisteif kqietvendh dastaaalea verrgemvvr vieprqmgrq
      181 aseaakkfia mqdpkhpihf veiikrpgqt lglyiregng vdrndgvfis rialetavyn
      241 sgclkvgdei lavnlvdvth mslddvviim siprrlvlat rhgqhqpvsh srqaehkapp
      301 vvvikrelne desdhatsnh vrdgnrrrgd gremlpsrsr lgltglgssq dlgssngdly
      361 ynsrpeghws yqpppppvit hqpkpsttqh fqpyergypk tleslaekdf tkvhsfytgp
      421 vmpsnggrrm stgagmqsvg srlssqtqss hygsygqhtg sgrimprsgs dqhlprvdyt
      481 sittparhtl lrsslksgts alryntrygt qgdsttsaqr qgqfgtltrr hrpsldyasd
      541 teatcssspk sayyyyrhnm nnpsqssavs hlatlsrsqi gqsssglrsn slprsgrtlp
      601 qqpglrsgls tvasglidqe dsdgalsape lpsirrdrgr ipsspsvfts deyrawlsrt
      661 pstsalyeqi rattarppry tysaenihaa anqgeygsyg gyrplsstld rlstrsasaq
      721 qvnlanlras taisstchrg ttnprpasva ssartsltsq kpslsssatq ratsvrrirn
      781 lldlestrsi ptptptrtqd qrlldinpae flkykiekpp tvgtpsstss llsslgetss
      841 gdlaggisgl lwvhllagrg lrsttsssaa ttpstpsgqp nlascglrdl ycvlecdrvh
      901 kartvvrtgd lmfdwdetfe ldlvgnrqld llvyswdpqy rhklcykgsv hlasllkesp
      961 lqqlavkvep rgtiylrlry idaqqtfrrr glpvislatr vaplfgvdld tvvsresktg
     1021 gvpggvstal amgvpnvpii vwrcveever rgldiiglyr lcgsatkkri lreafernar
     1081 svnlspdnvp dinvitgvlk dylrelpepl ftkclyqmmv dalavclpdd pqgsaklmfs
     1141 ildclpkvnr ctliylldhl amvvsqcnkm spaslavcfg pvlmlhseen gppldfqqpi
     1201 avlkylleiw pvksvrkiss vasalprvtq qaehnnsqhq mqqhqqqqqq qqqvphqmqg
     1261 tlgrtglpwq qqhslhqqpp tstaavlaas trppppvkpr qtfhflh
//
LOCUS       XP_034190651            1024 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608959 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190651
VERSION     XP_034190651.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190651.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1024
                     /product="uncharacterized protein LOC117608959 isoform X1"
                     /calculated_mol_wt=115292
     Region          115..141
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <216..>369
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     Region          645..674
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     CDS             1..1024
                     /gene="LOC117608959"
                     /coded_by="XM_034334760.2:312..3386"
                     /db_xref="GeneID:117608959"
ORIGIN      
        1 mkrrqrrpvl elnqshsgsh geyvqnkrrq lsndycgqnv panslanilc hynsdsdtee
       61 skkdvckldd qvnnffkeiq liapkqpvsd esavnpavns ntcqtnhvnq qalmwrecld
      121 essgypyywh ietnevtwem pdelrylknn vksssimkql qeshwvdfss vtyqqsqeni
      181 pegmiprevv arnrnryicn esakkreaqs dqevantsdt mdndsddgki emitsygsde
      241 sdsdtpddnq sknkapsvte ststgstvka knietypstt pviqpqlptv pqnsapdyen
      301 kdtveykkpe vsldlkpted tetkylkaqv kqsvtqetee ktnndnqtak esfvkkssnk
      361 hntdveldfr vslvpgyded sdaeeepetk qerkalfpip ltdnvkestp lpskivldes
      421 qtissivlin sadnessser kstqdqedeq vsekpeckee pavkaeedvq ktnkfldnls
      481 grskffqrkk riafdvtpsk tktvdesest kteaetqree snscdepitd hrehndttse
      541 nttenaanfk depnntleea nvssetsnes kedegevnll sqivleklkf lsegkpsvsp
      601 vqmmsiqlqt lciaweggyl eksylrrwls dtshelerle qdaappgwlc qwdrshkryy
      661 yqntttgvtq wtypdtgiag gaeemeicst pppteqeemi lpveeegvkl kskklqesde
      721 tsqdtaaprn sdieappppq ishpsppppp kiyaedlkrd krkqdkcndk ldskkqrpre
      781 egtiitevkl edsvapshss allspqptsl vnvtnaeplp pgvdlteapy eipatalkri
      841 iygavqpqgs alydatardp tvpilshpaa ivqehylqyp ayhqhlhapa alvlphkhsv
      901 qpaiqlipdy tpiytnhkii ekppvktake slvsaldsfy ndiarientg tekapqrpdt
      961 tiaepssvpa eetkleteqe plpaeiavke rkrkkarigi skkhkemssm vakwqkvqqn
     1021 fent
//
LOCUS       XP_034190652            1023 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608959 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190652
VERSION     XP_034190652.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334761.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190652.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1023
                     /product="uncharacterized protein LOC117608959 isoform X2"
                     /calculated_mol_wt=115193
     Region          115..141
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <216..>369
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     Region          645..674
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     CDS             1..1023
                     /gene="LOC117608959"
                     /coded_by="XM_034334761.2:314..3385"
                     /db_xref="GeneID:117608959"
ORIGIN      
        1 mkrrqrrpvl elnqshsgsh geyvqnkrrq lsndycgqnv panslanilc hynsdsdtee
       61 skkdvckldd qvnnffkeiq liapkqpvsd esavnpavns ntcqtnhvnq qalmwrecld
      121 essgypyywh ietnevtwem pdelrylknn vksssimkql qeshwvdfss vtyqqsqeni
      181 pegmiprevv arnrnryicn esakkreaqs dqevantsdt mdndsddgki emitsygsde
      241 sdsdtpddnq sknkapsvte ststgstvka knietypstt pviqpqlptv pqnsapdyen
      301 kdtveykkpe vsldlkpted tetkylkaqv kqsvtqetee ktnndnqtak esfvkkssnk
      361 hntdveldfr vslvpgyded sdaeeepetk qerkalfpip ltdnvkestp lpskivldes
      421 qtissivlin sadnessser kstqdqedeq vsekpeckee pavkaeedvq ktnkfldnls
      481 grskffqrkk riafdvtpsk tktvdesest kteaetqree snscdepitd hrehndttse
      541 nttenaanfk depnntleea nvssetsnes kedegevnll sqivleklkf lsegkpsvsp
      601 vqmmsiqlqt lciaweggyl eksylrrwls dtshelerle qdaappgwlc qwdrshkryy
      661 yqntttgvtq wtypdtgiag gaeemeicst pppteqeemi lpeeegvklk skklqesdet
      721 sqdtaaprns dieappppqi shpspppppk iyaedlkrdk rkqdkcndkl dskkqrpree
      781 gtiitevkle dsvapshssa llspqptslv nvtnaeplpp gvdlteapye ipatalkrii
      841 ygavqpqgsa lydatardpt vpilshpaai vqehylqypa yhqhlhapaa lvlphkhsvq
      901 paiqlipdyt piytnhkiie kppvktakes lvsaldsfyn diarientgt ekapqrpdtt
      961 iaepssvpae etkleteqep lpaeiavker krkkarigis kkhkemssmv akwqkvqqnf
     1021 ent
//
LOCUS       XP_034190239             633 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family F member 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190239
VERSION     XP_034190239.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334348.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="ATP-binding cassette sub-family F member 2"
                     /calculated_mol_wt=71529
     Region          95..613
                     /region_name="Uup"
                     /note="ATPase components of ABC transporters with
                     duplicated ATPase domains [General function prediction
                     only]; COG0488"
                     /db_xref="CDD:440254"
     CDS             1..633
                     /gene="LOC117608766"
                     /coded_by="XM_034334348.2:157..2058"
                     /db_xref="GeneID:117608766"
ORIGIN      
        1 mpsdakkkqq qkkkeaakar qsgkkpaqtp qtkgaengke gspgvgviqn gtngtpisae
       61 ealclklead arlnaearsc tgslashprs rdikisnfsi tfhgcellqd tmlelncgrr
      121 ygllglngsg kstllsvlgn revpipdqid ifhltrempa snktalecvm evdeerirle
      181 klaeelvece eedaqeqlmd vyerledmta dtaearaahi lhglgftakm qrtptkdfsg
      241 gwrmrialar alyvkphlll ldeptnhldl dacvwleeel ktykrilvii shsqdflngi
      301 ctniihvnkk qlkyysgnye afvktrmell enqakqynwe qdqiahmkty iarfghgsak
      361 larqaqskek tlakmvaqgl tekvvndkvl nfyfpscgti pppvimvqnv sfrynedspw
      421 iyknlefgid ldtrialvgp ngagkstllk llygdlvpts gmirknshlr igryhqhlhe
      481 lldldmspld ymmkafpdvk ereemrkiig rygltgrqqv cpirqlsdgq rcrvvfawla
      541 wqvphlllld eptnhldmet idaladaind fdggmvlvsh dfrlinqvae eiwvcengav
      601 tkwsgnildy kehlknkvln dnqkrqkdlh rsk
//
LOCUS       XP_034190242             233 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-conjugating enzyme E2 J2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190242
VERSION     XP_034190242.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334351.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="ubiquitin-conjugating enzyme E2 J2 isoform X1"
                     /calculated_mol_wt=26492
     Region          11..145
                     /region_name="UBCc_UBE2J"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 J1, J2 and
                     related proteins; cd23799"
                     /db_xref="CDD:467419"
     CDS             1..233
                     /gene="LOC117608767"
                     /coded_by="XM_034334351.2:774..1475"
                     /db_xref="GeneID:117608767"
ORIGIN      
        1 mnrttnsata rlkqdylrlk kdpvpyvlae pvpsnilewy ishyvvkgpe ktpyeggfyh
       61 gklifpgefp fqppsiymtt pngrfkvntr lclsisdfhp dtwnpawsvs tiltgllsfm
      121 ieksptlgsi nttdyekkql aaqsleynlk dkmfcelfpe tvevikaele hrkelekqar
      181 hqstasevss lirdqlqreq splhsaltnl iviigfaafa ytvkyvlrsi ame
//
LOCUS       XP_034190243             230 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-conjugating enzyme E2 J2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190243
VERSION     XP_034190243.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334352.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="ubiquitin-conjugating enzyme E2 J2 isoform X2"
                     /calculated_mol_wt=26128
     Region          11..142
                     /region_name="UBCc_UBE2J"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 J1, J2 and
                     related proteins; cd23799"
                     /db_xref="CDD:467419"
     CDS             1..230
                     /gene="LOC117608767"
                     /coded_by="XM_034334352.2:774..1466"
                     /db_xref="GeneID:117608767"
ORIGIN      
        1 mnrttnsata rlkqdylrlk kdpvpyvlae pvpsnilewh yvvkgpektp yeggfyhgkl
       61 ifpgefpfqp psiymttpng rfkvntrlcl sisdfhpdtw npawsvstil tgllsfmiek
      121 sptlgsintt dyekkqlaaq sleynlkdkm fcelfpetve vikaelehrk elekqarhqs
      181 tasevsslir dqlqreqspl hsaltnlivi igfaafaytv kyvlrsiame
//
LOCUS       XP_034190244             225 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 98 [Osmia lignaria lignaria].
ACCESSION   XP_034190244
VERSION     XP_034190244.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334353.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..225
                     /product="transmembrane protein 98"
                     /calculated_mol_wt=23611
     Region          <64..170
                     /region_name="GCIP"
                     /note="Grap2 and cyclin-D-interacting; pfam13324"
                     /db_xref="CDD:463844"
     CDS             1..225
                     /gene="Tmem98"
                     /coded_by="XM_034334353.2:160..837"
                     /db_xref="GeneID:117608768"
ORIGIN      
        1 mmsspvsisn tgttagaigm etvvavalga laavflgafl vllvickrqr cyynqkdspd
       61 lssdllegek fsglgldawe sdewlagaer wvddatglap lciavlrsch alassltaia
      121 gtvnsntvpl eivdvarrip prvddvvrsl yppldarlle arvaalvlav thlalvtkhg
      181 vsksharkla fidqalsdmd shlsilrnaa laqeiacsvp astpv
//
LOCUS       XP_076545806            1719 aa            linear   INV 03-SEP-2025
DEFINITION  neuralized E3 ubiquitin protein ligase 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545806
VERSION     XP_076545806.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689691.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1719
                     /product="neuralized E3 ubiquitin protein ligase 4 isoform
                     X1"
                     /calculated_mol_wt=189551
     Region          13..172
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          238..400
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          435..596
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          621..783
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          821..984
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          1057..1215
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          1368..>1485
                     /region_name="NosD"
                     /note="Periplasmic copper-binding protein (NosD);
                     pfam05048"
                     /db_xref="CDD:428281"
     CDS             1..1719
                     /gene="Neurl4"
                     /coded_by="XM_076689691.1:252..5411"
                     /db_xref="GeneID:117606627"
ORIGIN      
        1 mstvgaqqiv dmfhqrcghr vtltnnncta irdfleynyg lvlsaeplkd dqlfevridk
       61 klkswsgsie igvtecdpei ielparatnl cqgtwimtns givhdgvrtv etygmnlnal
      121 eagstlgvmr tsnhelifyi ngisqdvavs niperifavv dmygdcvqvt itypqiaaal
      181 csepkdevei nndyalgeas nssttnlvan lnvnlnvnvn vnlpknpnpa avredrlrfh
      241 ervgslvkls nnartaerrr pldefnngvv mthrplrdne lfevridrll hkwsgsievg
      301 vtmhsptale fpatmtnmrs gttmmsgcgi lvngkgtcre ygefnldelr egdrvgmirr
      361 sngnlhylin gldqgiaakv pagvwgvidl ygmtvkvtiv drdereeqnl vtrrntlhlq
      421 glnedeeepp drlmfhsccg thvevinngr tahrpnvmdd fnngvvltsr plkpnelfev
      481 rldkivtkwa gsieigvtth sptelefpft mtnvrsgtwm mtengvmhng ttiidqygqn
      541 ldrlqvgdrv gvmrkdnatl hfyvngadqg aaamnvperv ygvidlygqa aqativdntd
      601 fyspttnnss fsnttlysdl rfhhihgkna kiinngltal rpralgefne aivianrplr
      661 dgemfevtid kmvdrwtgai eagvtlirpd elefpstmtd idhdtwmlsg snvmrdgvtl
      721 tnnyacdldk lvegnrigmm rcsdsslhyy ldgvdqgpac tglpphvypv idlygqcvqv
      781 tivlperrdp mtqqylpsen stsqqptsvi qlqaqteimh kfhesvglsi qlnsdrtvat
      841 rcreysnavl lsetplenne ifeitiqeva rewsgclkig vmsnetgnwl tsmnlvpgig
      901 sippdawylt gnevrhngyv lcmnycpsle wlrvgdkigm krthegnlkf yingedmgia
      961 asnipemvys vvelfgstva vnitsskqqn tavspnaslr lqdslellld pmppvlrnda
     1021 gmdtsadvse gklvndvtlt ptqttphlvg esdwsyefhe nhgrniqlet ktiarrvasy
     1081 nqgvvmssrp likgkpfsvk ieklnerwvs nilcgvtcis pekanfplta lgfkkhswii
     1141 csdwishngi rvktkygaal enlqsnsivg lfidednrlr liingvdqgv aatdlppyvy
     1201 avfdlygqce qisivgnnve pfsstsidnt vtsvecirtk ledaensrek adlecheken
     1261 vvaatssdls sstspsvpsq cssnvknddt veyqgtcnng svhlmnnien kampnnsdsn
     1321 nlesssgmsn datsrtvknk nydnsnqlnn saisnsqsen snnrnectnv einnatnini
     1381 kngtanstsn itnmntnann vinesiasns qgvnlnathq nnasinmlss sqntlsngas
     1441 dmsntvssgf neirtnvawn ntvsvnnsig gnttisgqnt asihnapslq llqisslpst
     1501 platntvpsk kceylkacmr lkkslvlpde ffslddvlcy csacykvegd gavckkgepp
     1561 aefavpigwt rfplrqsina nqipqgttdk whvafygirl dairlildtg elmtkeqldv
     1621 snltmnikae dqnpqvtfsp sikyaaseef trkypyidtq snkklnasta fqllvrpgsy
     1681 tispggkdgn dpqfesskws tkeagatvim alliqldgf
//
LOCUS       XP_076545807            1662 aa            linear   INV 03-SEP-2025
DEFINITION  neuralized E3 ubiquitin protein ligase 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545807
VERSION     XP_076545807.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689692.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1662
                     /product="neuralized E3 ubiquitin protein ligase 4 isoform
                     X2"
                     /calculated_mol_wt=183515
     Region          13..172
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          238..400
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          435..596
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          621..783
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          821..984
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          1057..1215
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          1368..>1485
                     /region_name="NosD"
                     /note="Periplasmic copper-binding protein (NosD);
                     pfam05048"
                     /db_xref="CDD:428281"
     CDS             1..1662
                     /gene="Neurl4"
                     /coded_by="XM_076689692.1:252..5240"
                     /db_xref="GeneID:117606627"
ORIGIN      
        1 mstvgaqqiv dmfhqrcghr vtltnnncta irdfleynyg lvlsaeplkd dqlfevridk
       61 klkswsgsie igvtecdpei ielparatnl cqgtwimtns givhdgvrtv etygmnlnal
      121 eagstlgvmr tsnhelifyi ngisqdvavs niperifavv dmygdcvqvt itypqiaaal
      181 csepkdevei nndyalgeas nssttnlvan lnvnlnvnvn vnlpknpnpa avredrlrfh
      241 ervgslvkls nnartaerrr pldefnngvv mthrplrdne lfevridrll hkwsgsievg
      301 vtmhsptale fpatmtnmrs gttmmsgcgi lvngkgtcre ygefnldelr egdrvgmirr
      361 sngnlhylin gldqgiaakv pagvwgvidl ygmtvkvtiv drdereeqnl vtrrntlhlq
      421 glnedeeepp drlmfhsccg thvevinngr tahrpnvmdd fnngvvltsr plkpnelfev
      481 rldkivtkwa gsieigvtth sptelefpft mtnvrsgtwm mtengvmhng ttiidqygqn
      541 ldrlqvgdrv gvmrkdnatl hfyvngadqg aaamnvperv ygvidlygqa aqativdntd
      601 fyspttnnss fsnttlysdl rfhhihgkna kiinngltal rpralgefne aivianrplr
      661 dgemfevtid kmvdrwtgai eagvtlirpd elefpstmtd idhdtwmlsg snvmrdgvtl
      721 tnnyacdldk lvegnrigmm rcsdsslhyy ldgvdqgpac tglpphvypv idlygqcvqv
      781 tivlperrdp mtqqylpsen stsqqptsvi qlqaqteimh kfhesvglsi qlnsdrtvat
      841 rcreysnavl lsetplenne ifeitiqeva rewsgclkig vmsnetgnwl tsmnlvpgig
      901 sippdawylt gnevrhngyv lcmnycpsle wlrvgdkigm krthegnlkf yingedmgia
      961 asnipemvys vvelfgstva vnitsskqqn tavspnaslr lqdslellld pmppvlrnda
     1021 gmdtsadvse gklvndvtlt ptqttphlvg esdwsyefhe nhgrniqlet ktiarrvasy
     1081 nqgvvmssrp likgkpfsvk ieklnerwvs nilcgvtcis pekanfplta lgfkkhswii
     1141 csdwishngi rvktkygaal enlqsnsivg lfidednrlr liingvdqgv aatdlppyvy
     1201 avfdlygqce qisivgnnve pfsstsidnt vtsvecirtk ledaensrek adlecheken
     1261 vvaatssdls sstspsvpsq cssnvknddt veyqgtcnng svhlmnnien kampnnsdsn
     1321 nlesssgmsn datsrtvknk nydnsnqlnn saisnsqsen snnrnectnv einnatnini
     1381 kngtanstsn itnmntnann vinesiasns qgvnlnathq nnasinmlss sqntlsngas
     1441 dmsntvssgf neirtnvawn ntvsvnnsig gnttisgqnt asihnapslq llqisslpst
     1501 platntvpsk kceylkacmr lkkslvlpde ffslddvlcy csacykvegd gavckkgepp
     1561 aefavpigwt rfplrqsina nqipqgttdk whvafygirl dairlildtg elmtkeqldv
     1621 snltmnikae dqnpqvtfsp sikyaaseef trkypkarfv hd
//
LOCUS       XP_076545808            1454 aa            linear   INV 03-SEP-2025
DEFINITION  GIGYF family protein Gyf isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545808
VERSION     XP_076545808.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689693.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1454
                     /product="GIGYF family protein Gyf isoform X1"
                     /calculated_mol_wt=164455
     Region          381..520
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          603..647
                     /region_name="GYF"
                     /note="GYF domain; pfam02213"
                     /db_xref="CDD:460496"
     Site            614..630
                     /site_type="active"
                     /note="proline binding motif [active]"
                     /db_xref="CDD:238027"
     Site            order(624,629..630)
                     /site_type="active"
                     /note="proline interaction residues [active]"
                     /db_xref="CDD:238027"
     Region          <908..>966
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     CDS             1..1454
                     /gene="LOC143305575"
                     /coded_by="XM_076689693.1:91..4455"
                     /db_xref="GeneID:143305575"
ORIGIN      
        1 mvtrktlllv tfrcvrytnd lrmrfipkkn hqpywcvvsl ssviskianl imtdsmkfgp
       61 ewlrnlsgdn cnnsgggggs tslttpryql aehrygreem lilfdrnckp pepltsfssl
      121 yvektqlpla liqmtedetr mwnggitntv grgrggsvdr gvrgrngrgs lytshysrgv
      181 gfddsgdgvr idsqpfqarn rtfdrsqser gwserngate pgewngstsp rkelnrgasg
      241 gslmegnwrr hrgggedddg wrkwgrsswr eggsmdrdrl drneegeesr nsggrwehrg
      301 thrtthdssh hppprtariw esnhhdnnhd nlpewatenp gesgsfdasg afhggmysdd
      361 dedgvisagg nqetrtrrvs egsaantrsg nkhlaytssq aqtanrnpnn gtstitkers
      421 kslhlldnkd epvekekisn spvkpsvatt vntptktsss vslessqnks nvatsteqsd
      481 vkkippndse nnkspgktqt kvdeaetktd pmatgkkqip lrtepqkpkh slfklacqkl
      541 nsdidntkkk teddfdrmke eadalvaklm adeenhrekt ssvptsignq sstvpstngq
      601 ekwfyrdpqg evqgpflase maewckagyf taglmvrrtc deryttlgdl mkmcgripft
      661 pgppipplkl tdqvippvpn avptgisalp ktgiedplll lqyqqmrllq nqqlllrqmr
      721 tsaiaklsqs ehwatlspme qnqlifqyvf qdsemsevpi stnpfvshlp sqpsnpvmql
      781 ftqmqqaktq peahlpstpr sttpahppam dpiqqliqqm ggmqnvsgiq qsnissapsa
      841 tqednpiksl lrqlnvntng hpqaahidtv wpqpppqinp qfnaqnwlaq vgpipavppg
      901 qlptslwdlh tkeikteqqi ldeqnlrlqe drkkeelrkq eelqrqaeee sakrkkeeqa
      961 kqaeeakrkd eerkkkeeek krkdeekrkq eeerkkkeek rrkeeekkre ekrkqeeeil
     1021 rkkqeeekrk keelkkleek qkkeeerrkk leeeqrkqee ermrkeeear kqaeaeeqar
     1081 raeqrrreae alrklqersk apwaqaprap aataapaasl aeiqrlerek kaeeqrfqqm
     1141 mqqqlaqqka teaaqealat dsskrlqfkw aekatsstkp lqvkslaqiq qeeqeritkv
     1201 kqqererqek agqkesanvl qnagiwgtas qclnwansga snvgqawsnn sgtsgfwdep
     1261 tpvksssatk qsakqtavik spsgnqqqqq snkanksknk reeelvkklf eqntaktddf
     1321 tqwcnkalsg lqvsvdiptf vgflrdiesa yevkeyvrdy lgdnkqslef akqflekrsk
     1381 wrsaqrpqae addlckpapa vnpnapaefq evkgkskkpk kgkmykvdnr ilgfsvtaap
     1441 drinvgdrdy gegv
//
LOCUS       XP_076545809            1453 aa            linear   INV 03-SEP-2025
DEFINITION  GIGYF family protein Gyf isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545809
VERSION     XP_076545809.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689694.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1453
                     /product="GIGYF family protein Gyf isoform X2"
                     /calculated_mol_wt=164327
     Region          381..520
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          603..647
                     /region_name="GYF"
                     /note="GYF domain; pfam02213"
                     /db_xref="CDD:460496"
     Site            614..630
                     /site_type="active"
                     /note="proline binding motif [active]"
                     /db_xref="CDD:238027"
     Site            order(624,629..630)
                     /site_type="active"
                     /note="proline interaction residues [active]"
                     /db_xref="CDD:238027"
     Region          <908..>966
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     CDS             1..1453
                     /gene="LOC143305575"
                     /coded_by="XM_076689694.1:92..4453"
                     /db_xref="GeneID:143305575"
ORIGIN      
        1 mvtrktlllv tfrcvrytnd lrmrfipkkn hqpywcvvsl ssviskianl imtdsmkfgp
       61 ewlrnlsgdn cnnsgggggs tslttpryql aehrygreem lilfdrnckp pepltsfssl
      121 yvektqlpla liqmtedetr mwnggitntv grgrggsvdr gvrgrngrgs lytshysrgv
      181 gfddsgdgvr idsqpfqarn rtfdrsqser gwserngate pgewngstsp rkelnrgasg
      241 gslmegnwrr hrgggedddg wrkwgrsswr eggsmdrdrl drneegeesr nsggrwehrg
      301 thrtthdssh hppprtariw esnhhdnnhd nlpewatenp gesgsfdasg afhggmysdd
      361 dedgvisagg nqetrtrrvs egsaantrsg nkhlaytssq aqtanrnpnn gtstitkers
      421 kslhlldnkd epvekekisn spvkpsvatt vntptktsss vslessqnks nvatsteqsd
      481 vkkippndse nnkspgktqt kvdeaetktd pmatgkkqip lrtepqkpkh slfklacqkl
      541 nsdidntkkk teddfdrmke eadalvaklm adeenhrekt ssvptsignq sstvpstngq
      601 ekwfyrdpqg evqgpflase maewckagyf taglmvrrtc deryttlgdl mkmcgripft
      661 pgppipplkl tdqvippvpn avptgisalp ktgiedplll lqyqqmrllq nqqlllrqmr
      721 tsaiaklsqs ehwatlspme qnqlifqyvf qdsemsevpi stnpfvshlp sqpsnpvmql
      781 ftqmqqaktq peahlpstpr sttpahppam dpiqqliqqm ggmqnvsgiq qsnissapsa
      841 tqednpiksl lrqlnvntng hpqaahidtv wpqpppqinp qfnaqnwlaq vgpipavppg
      901 qlptslwdlh tkeikteqqi ldeqnlrlqe drkkeelrkq eelqrqaeee sakrkkeeqa
      961 kqaeeakrkd eerkkkeeek krkdeekrkq eeerkkkeek rrkeeekkre ekrkqeeeil
     1021 rkkqeeekrk keelkkleek qkkeeerrkk leeeqrkqee ermrkeeear kqaeaeeqar
     1081 raeqrrreae alrklqersk apwaqaprap aataapaasl aeiqrlerek kaeeqrfqqm
     1141 mqqqlaqqka teaaqealat dsskrlqfkw aekatsstkp lqvkslaqiq qeeqeritkv
     1201 kqererqeka gqkesanvlq nagiwgtasq clnwansgas nvgqawsnns gtsgfwdept
     1261 pvksssatkq sakqtaviks psgnqqqqqs nkanksknkr eeelvkklfe qntaktddft
     1321 qwcnkalsgl qvsvdiptfv gflrdiesay evkeyvrdyl gdnkqslefa kqflekrskw
     1381 rsaqrpqaea ddlckpapav npnapaefqe vkgkskkpkk gkmykvdnri lgfsvtaapd
     1441 rinvgdrdyg egv
//
LOCUS       XP_076545810            1452 aa            linear   INV 03-SEP-2025
DEFINITION  GIGYF family protein Gyf isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545810
VERSION     XP_076545810.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689695.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1452
                     /product="GIGYF family protein Gyf isoform X3"
                     /calculated_mol_wt=164228
     Region          381..520
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          603..647
                     /region_name="GYF"
                     /note="GYF domain; pfam02213"
                     /db_xref="CDD:460496"
     Site            614..630
                     /site_type="active"
                     /note="proline binding motif [active]"
                     /db_xref="CDD:238027"
     Site            order(624,629..630)
                     /site_type="active"
                     /note="proline interaction residues [active]"
                     /db_xref="CDD:238027"
     Region          <908..>966
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     CDS             1..1452
                     /gene="LOC143305575"
                     /coded_by="XM_076689695.1:92..4450"
                     /db_xref="GeneID:143305575"
ORIGIN      
        1 mvtrktlllv tfrcvrytnd lrmrfipkkn hqpywcvvsl ssviskianl imtdsmkfgp
       61 ewlrnlsgdn cnnsgggggs tslttpryql aehrygreem lilfdrnckp pepltsfssl
      121 yvektqlpla liqmtedetr mwnggitntv grgrggsvdr gvrgrngrgs lytshysrgv
      181 gfddsgdgvr idsqpfqarn rtfdrsqser gwserngate pgewngstsp rkelnrgasg
      241 gslmegnwrr hrgggedddg wrkwgrsswr eggsmdrdrl drneegeesr nsggrwehrg
      301 thrtthdssh hppprtariw esnhhdnnhd nlpewatenp gesgsfdasg afhggmysdd
      361 dedgvisagg nqetrtrrvs egsaantrsg nkhlaytssq aqtanrnpnn gtstitkers
      421 kslhlldnkd epvekekisn spvkpsvatt vntptktsss vslessqnks nvatsteqsd
      481 vkkippndse nnkspgktqt kvdeaetktd pmatgkkqip lrtepqkpkh slfklacqkl
      541 nsdidntkkk teddfdrmke eadalvaklm adeenhrekt ssvptsignq sstvpstngq
      601 ekwfyrdpqg evqgpflase maewckagyf taglmvrrtc deryttlgdl mkmcgripft
      661 pgppipplkl tdqvippvpn avptgisalp ktgiedplll lqyqqmrllq nqqlllrqmr
      721 tsaiaklsqs ehwatlspme qnqlifqyvf qdsemsevpi stnpfvshlp sqpsnpvmql
      781 ftqmqqaktq peahlpstpr sttpahppam dpiqqliqqm ggmqnvsgiq qsnissapsa
      841 tqednpiksl lrqlnvntng hpqaahidtv wpqpppqinp qfnaqnwlaq vgpipavppg
      901 qlptslwdlh tkeikteqqi ldeqnlrlqe drkkeelrkq eelqrqaeee sakrkkeeqa
      961 kqaeeakrkd eerkkkeeek krkdeekrkq eeerkkkeek rrkeeekkre ekrkqeeeil
     1021 rkkqeeekrk keelkkleek qkkeeerrkk leeeqrkqee ermrkeeear kqaeaeeqar
     1081 raeqrrreae alrklqersk apwaqaprap aataapaasl aeiqrlerek kaeeqrfqqm
     1141 mqqqlaqqka teaaqealat dsskrlqfkw aekatsstkp lqvkslaqiq qeeqeritkq
     1201 qererqekag qkesanvlqn agiwgtasqc lnwansgasn vgqawsnnsg tsgfwdeptp
     1261 vksssatkqs akqtaviksp sgnqqqqqsn kanksknkre eelvkklfeq ntaktddftq
     1321 wcnkalsglq vsvdiptfvg flrdiesaye vkeyvrdylg dnkqslefak qflekrskwr
     1381 saqrpqaead dlckpapavn pnapaefqev kgkskkpkkg kmykvdnril gfsvtaapdr
     1441 invgdrdyge gv
//
LOCUS       XP_076545811            1451 aa            linear   INV 03-SEP-2025
DEFINITION  GIGYF family protein Gyf isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076545811
VERSION     XP_076545811.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689696.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1451
                     /product="GIGYF family protein Gyf isoform X4"
                     /calculated_mol_wt=164099
     Region          381..520
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          603..647
                     /region_name="GYF"
                     /note="GYF domain; pfam02213"
                     /db_xref="CDD:460496"
     Site            614..630
                     /site_type="active"
                     /note="proline binding motif [active]"
                     /db_xref="CDD:238027"
     Site            order(624,629..630)
                     /site_type="active"
                     /note="proline interaction residues [active]"
                     /db_xref="CDD:238027"
     Region          <908..>966
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     CDS             1..1451
                     /gene="LOC143305575"
                     /coded_by="XM_076689696.1:92..4447"
                     /db_xref="GeneID:143305575"
ORIGIN      
        1 mvtrktlllv tfrcvrytnd lrmrfipkkn hqpywcvvsl ssviskianl imtdsmkfgp
       61 ewlrnlsgdn cnnsgggggs tslttpryql aehrygreem lilfdrnckp pepltsfssl
      121 yvektqlpla liqmtedetr mwnggitntv grgrggsvdr gvrgrngrgs lytshysrgv
      181 gfddsgdgvr idsqpfqarn rtfdrsqser gwserngate pgewngstsp rkelnrgasg
      241 gslmegnwrr hrgggedddg wrkwgrsswr eggsmdrdrl drneegeesr nsggrwehrg
      301 thrtthdssh hppprtariw esnhhdnnhd nlpewatenp gesgsfdasg afhggmysdd
      361 dedgvisagg nqetrtrrvs egsaantrsg nkhlaytssq aqtanrnpnn gtstitkers
      421 kslhlldnkd epvekekisn spvkpsvatt vntptktsss vslessqnks nvatsteqsd
      481 vkkippndse nnkspgktqt kvdeaetktd pmatgkkqip lrtepqkpkh slfklacqkl
      541 nsdidntkkk teddfdrmke eadalvaklm adeenhrekt ssvptsignq sstvpstngq
      601 ekwfyrdpqg evqgpflase maewckagyf taglmvrrtc deryttlgdl mkmcgripft
      661 pgppipplkl tdqvippvpn avptgisalp ktgiedplll lqyqqmrllq nqqlllrqmr
      721 tsaiaklsqs ehwatlspme qnqlifqyvf qdsemsevpi stnpfvshlp sqpsnpvmql
      781 ftqmqqaktq peahlpstpr sttpahppam dpiqqliqqm ggmqnvsgiq qsnissapsa
      841 tqednpiksl lrqlnvntng hpqaahidtv wpqpppqinp qfnaqnwlaq vgpipavppg
      901 qlptslwdlh tkeikteqqi ldeqnlrlqe drkkeelrkq eelqrqaeee sakrkkeeqa
      961 kqaeeakrkd eerkkkeeek krkdeekrkq eeerkkkeek rrkeeekkre ekrkqeeeil
     1021 rkkqeeekrk keelkkleek qkkeeerrkk leeeqrkqee ermrkeeear kqaeaeeqar
     1081 raeqrrreae alrklqersk apwaqaprap aataapaasl aeiqrlerek kaeeqrfqqm
     1141 mqqqlaqqka teaaqealat dsskrlqfkw aekatsstkp lqvkslaqiq qeeqeritkq
     1201 ererqekagq kesanvlqna giwgtasqcl nwansgasnv gqawsnnsgt sgfwdeptpv
     1261 ksssatkqsa kqtaviksps gnqqqqqsnk anksknkree elvkklfeqn taktddftqw
     1321 cnkalsglqv svdiptfvgf lrdiesayev keyvrdylgd nkqslefakq flekrskwrs
     1381 aqrpqaeadd lckpapavnp napaefqevk gkskkpkkgk mykvdnrilg fsvtaapdri
     1441 nvgdrdygeg v
//
LOCUS       XP_076545812            1422 aa            linear   INV 03-SEP-2025
DEFINITION  GIGYF family protein Gyf isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076545812
VERSION     XP_076545812.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689697.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1422
                     /product="GIGYF family protein Gyf isoform X5"
                     /calculated_mol_wt=160546
     Region          349..488
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          571..615
                     /region_name="GYF"
                     /note="GYF domain; pfam02213"
                     /db_xref="CDD:460496"
     Site            582..598
                     /site_type="active"
                     /note="proline binding motif [active]"
                     /db_xref="CDD:238027"
     Site            order(592,597..598)
                     /site_type="active"
                     /note="proline interaction residues [active]"
                     /db_xref="CDD:238027"
     Region          <876..>934
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     CDS             1..1422
                     /gene="LOC143305575"
                     /coded_by="XM_076689697.1:573..4841"
                     /db_xref="GeneID:143305575"
ORIGIN      
        1 mlintcslln fhratanlim tdsmkfgpew lrnlsgdncn nsgggggsts lttpryqlae
       61 hrygreemli lfdrnckppe pltsfsslyv ektqlplali qmtedetrmw nggitntvgr
      121 grggsvdrgv rgrngrgsly tshysrgvgf ddsgdgvrid sqpfqarnrt fdrsqsergw
      181 serngatepg ewngstsprk elnrgasggs lmegnwrrhr gggedddgwr kwgrsswreg
      241 gsmdrdrldr neegeesrns ggrwehrgth rtthdsshhp pprtariwes nhhdnnhdnl
      301 pewatenpge sgsfdasgaf hggmysddde dgvisaggnq etrtrrvseg saantrsgnk
      361 hlaytssqaq tanrnpnngt stitkersks lhlldnkdep vekekisnsp vkpsvattvn
      421 tptktsssvs lessqnksnv atsteqsdvk kippndsenn kspgktqtkv deaetktdpm
      481 atgkkqiplr tepqkpkhsl fklacqklns didntkkkte ddfdrmkeea dalvaklmad
      541 eenhrektss vptsignqss tvpstngqek wfyrdpqgev qgpflasema ewckagyfta
      601 glmvrrtcde ryttlgdlmk mcgripftpg ppipplkltd qvippvpnav ptgisalpkt
      661 giedpllllq yqqmrllqnq qlllrqmrts aiaklsqseh watlspmeqn qlifqyvfqd
      721 semsevpist npfvshlpsq psnpvmqlft qmqqaktqpe ahlpstprst tpahppamdp
      781 iqqliqqmgg mqnvsgiqqs nissapsatq ednpiksllr qlnvntnghp qaahidtvwp
      841 qpppqinpqf naqnwlaqvg pipavppgql ptslwdlhtk eikteqqild eqnlrlqedr
      901 kkeelrkqee lqrqaeeesa krkkeeqakq aeeakrkdee rkkkeeekkr kdeekrkqee
      961 erkkkeekrr keeekkreek rkqeeeilrk kqeeekrkke elkkleekqk keeerrkkle
     1021 eeqrkqeeer mrkeeearkq aeaeeqarra eqrrreaeal rklqerskap waqaprapaa
     1081 taapaaslae iqrlerekka eeqrfqqmmq qqlaqqkate aaqealatds skrlqfkwae
     1141 katsstkplq vkslaqiqqe eqeritkvkq qererqekag qkesanvlqn agiwgtasqc
     1201 lnwansgasn vgqawsnnsg tsgfwdeptp vksssatkqs akqtaviksp sgnqqqqqsn
     1261 kanksknkre eelvkklfeq ntaktddftq wcnkalsglq vsvdiptfvg flrdiesaye
     1321 vkeyvrdylg dnkqslefak qflekrskwr saqrpqaead dlckpapavn pnapaefqev
     1381 kgkskkpkkg kmykvdnril gfsvtaapdr invgdrdyge gv
//
LOCUS       XP_076545813            1403 aa            linear   INV 03-SEP-2025
DEFINITION  GIGYF family protein Gyf isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076545813
VERSION     XP_076545813.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689698.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1403
                     /product="GIGYF family protein Gyf isoform X6"
                     /calculated_mol_wt=158418
     Region          330..469
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          552..596
                     /region_name="GYF"
                     /note="GYF domain; pfam02213"
                     /db_xref="CDD:460496"
     Site            563..579
                     /site_type="active"
                     /note="proline binding motif [active]"
                     /db_xref="CDD:238027"
     Site            order(573,578..579)
                     /site_type="active"
                     /note="proline interaction residues [active]"
                     /db_xref="CDD:238027"
     Region          <857..>915
                     /region_name="tolA"
                     /note="cell envelope integrity inner membrane protein
                     TolA; Provisional; PRK09510"
                     /db_xref="CDD:236545"
     CDS             1..1403
                     /gene="LOC143305575"
                     /coded_by="XM_076689698.1:91..4302"
                     /db_xref="GeneID:143305575"
ORIGIN      
        1 mtdsmkfgpe wlrnlsgdnc nnsgggggst slttpryqla ehrygreeml ilfdrnckpp
       61 epltsfssly vektqlplal iqmtedetrm wnggitntvg rgrggsvdrg vrgrngrgsl
      121 ytshysrgvg fddsgdgvri dsqpfqarnr tfdrsqserg wserngatep gewngstspr
      181 kelnrgasgg slmegnwrrh rgggedddgw rkwgrsswre ggsmdrdrld rneegeesrn
      241 sggrwehrgt hrtthdsshh ppprtariwe snhhdnnhdn lpewatenpg esgsfdasga
      301 fhggmysddd edgvisaggn qetrtrrvse gsaantrsgn khlaytssqa qtanrnpnng
      361 tstitkersk slhlldnkde pvekekisns pvkpsvattv ntptktsssv slessqnksn
      421 vatsteqsdv kkippndsen nkspgktqtk vdeaetktdp matgkkqipl rtepqkpkhs
      481 lfklacqkln sdidntkkkt eddfdrmkee adalvaklma deenhrekts svptsignqs
      541 stvpstngqe kwfyrdpqge vqgpflasem aewckagyft aglmvrrtcd eryttlgdlm
      601 kmcgripftp gppipplklt dqvippvpna vptgisalpk tgiedpllll qyqqmrllqn
      661 qqlllrqmrt saiaklsqse hwatlspmeq nqlifqyvfq dsemsevpis tnpfvshlps
      721 qpsnpvmqlf tqmqqaktqp eahlpstprs ttpahppamd piqqliqqmg gmqnvsgiqq
      781 snissapsat qednpiksll rqlnvntngh pqaahidtvw pqpppqinpq fnaqnwlaqv
      841 gpipavppgq lptslwdlht keikteqqil deqnlrlqed rkkeelrkqe elqrqaeees
      901 akrkkeeqak qaeeakrkde erkkkeeekk rkdeekrkqe eerkkkeekr rkeeekkree
      961 krkqeeeilr kkqeeekrkk eelkkleekq kkeeerrkkl eeeqrkqeee rmrkeeeark
     1021 qaeaeeqarr aeqrrreaea lrklqerska pwaqaprapa ataapaasla eiqrlerekk
     1081 aeeqrfqqmm qqqlaqqkat eaaqealatd sskrlqfkwa ekatsstkpl qvkslaqiqq
     1141 eeqeritkvk qqererqeka gqkesanvlq nagiwgtasq clnwansgas nvgqawsnns
     1201 gtsgfwdept pvksssatkq sakqtaviks psgnqqqqqs nkanksknkr eeelvkklfe
     1261 qntaktddft qwcnkalsgl qvsvdiptfv gflrdiesay evkeyvrdyl gdnkqslefa
     1321 kqflekrskw rsaqrpqaea ddlckpapav npnapaefqe vkgkskkpkk gkmykvdnri
     1381 lgfsvtaapd rinvgdrdyg egv
//
LOCUS       XP_034185408             736 aa            linear   INV 03-SEP-2025
DEFINITION  procollagen lysyl hydroxylase [Osmia lignaria lignaria].
ACCESSION   XP_034185408
VERSION     XP_034185408.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329517.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185408.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="procollagen lysyl hydroxylase"
                     /calculated_mol_wt=84754
     Region          31..263
                     /region_name="GT_LH"
                     /note="catalytic glycosyltransferase (GT) domain found in
                     the lysyl hydroxylase (LH) family; cl45890"
                     /db_xref="CDD:459235"
     Site            order(38..40,71,86,109..110,112,250,252)
                     /site_type="other"
                     /note="UDP binding site [chemical binding]"
                     /db_xref="CDD:438557"
     Site            order(109,112,250)
                     /site_type="other"
                     /note="Mn binding site [ion binding]"
                     /db_xref="CDD:438557"
     Region          571..734
                     /region_name="P4Hc"
                     /note="Prolyl 4-hydroxylase alpha subunit homologues;
                     smart00702"
                     /db_xref="CDD:214780"
     CDS             1..736
                     /gene="Plod"
                     /coded_by="XM_034329517.2:204..2414"
                     /db_xref="GeneID:117606709"
ORIGIN      
        1 mrpknvgcci lwslfltyhv vsetppstdt ndvlvvtvat netdgykryl rsvevygfrd
       61 nlkvlgmgnp wlggkniktg rgggykinll keaiekyknd dqkiiiftds ydviflagls
      121 eiiakfkstd arvlfsaegs cwpdrslatk ypavsggkrf lnsggfigya sdvhailtys
      181 plknedddql fytmayldek lreqhkikld hkseifqnly gavadvelkf egaktslvnt
      241 vynteplilh gngysklsln slgnylanaw speegcitcw egtmeldktl pdtypiilia
      301 vfierptpfm eeffqkiydq aypksklhlf ihntvdyhqg viddflmkfe keyrsskvil
      361 pkdsisesda rnlamdycll kkcsgyftvd slahldheyt lkylvdqhrg iiapllvrpy
      421 kmwsnfwgai mddgfyarsf dymeivkner rglwnvpfin tcylinatli snketrpsye
      481 egdldtdmaf aysnrersif myvsnradfg hlvdpdsydv tlthpdlyqi fdnkldwekk
      541 ylhvnyslnf qpeqipiqpc pdvywfpvvn ekftkslidv meafgkwsdg snndprltgg
      601 yenvptrdih mnqvkfephw lhilkeyirp lqehvftgyf hdppralmnf vvryrpdeqp
      661 slkphhdsst ytvnialnkv gvdyegggcr firyncsvtd tkpgwmlmhp grlthyhegl
      721 rvtsgtryim isfvdp
//
LOCUS       XP_034185409             735 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606710 [Osmia lignaria lignaria].
ACCESSION   XP_034185409
VERSION     XP_034185409.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329518.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185409.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..735
                     /product="uncharacterized protein LOC117606710"
                     /calculated_mol_wt=85069
     Region          5..53
                     /region_name="HTH_48"
                     /note="HTH domain in Mos1 transposase; pfam17906"
                     /db_xref="CDD:465558"
     Region          41..316
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     Region          42..108
                     /region_name="HTH_32"
                     /note="Homeodomain-like domain; pfam13565"
                     /db_xref="CDD:463923"
     Region          <457..686
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          532..609
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(537,555..556,559,578..579,581..582,585,592,601..603,
                     605)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410788"
     CDS             1..735
                     /gene="LOC117606710"
                     /coded_by="XM_034329518.2:1..2208"
                     /db_xref="GeneID:117606710"
ORIGIN      
        1 mevsrenfra mifydfkigl nkiqscarlr aafgdsspst atiyrwfkef qngresllde
       61 dragrpvtav tdenvfavkq mleednraty aqiqatlrig saavrtilhd rlrtrklcsr
      121 wvphhltneq krarvkwcqe mlqrfdngcs nevsniittd eswiyqyend lktewiipge
      181 tfpmkgrrig kkmvcsffca gghietitle dqsngdakwy ttvclaqvfd kfrkkrlkte
      241 dqkeiilhhn tllhsfnetv aflnklgvsv lahppdspdl apsdfflfpk vkdsvrgqrf
      301 etsetavaay keflnsipke ewrvefdkwf drmkscircr geycekiycn fdsdlspisp
      361 iprteiqqts nnerrnisfe egvkkekidv eednalpenl ndkehqnkkt dgnkdedenk
      421 ievkkeqvne nedtmigqye mvlvkmeenf edeqetsksv mpesasprct kvpetngmse
      481 irarslkmlq nliqtsqkks cnnnsqinse atsgindnes dqsdndtrsy lkppypyhcl
      541 iamalknsps gtattteiyd fmrrhfpyfk ttpdrwkdsi thylwfnknf vkclgyehqq
      601 kgcrwtfapg eaammdeklq klskedrlai ekamvhpehl dllergemky wqstcsknad
      661 eesssnddvk eesiqdraat nsainsvnkd svkkesvnct krqrrrnmyq rgverpnvpl
      721 llrgsisncc sinie
//
LOCUS       XP_034185411             460 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class A isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185411
VERSION     XP_034185411.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329520.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class A isoform X1"
                     /calculated_mol_wt=52105
     Region          28..415
                     /region_name="GT4_PIG-A-like"
                     /note="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit A and similar
                     proteins; cd03796"
                     /db_xref="CDD:340827"
     Site            order(39,223..225,287,309)
                     /site_type="other"
                     /note="putative ADP-binding pocket [chemical binding]"
                     /db_xref="CDD:340827"
     CDS             1..460
                     /gene="PIG-A"
                     /coded_by="XM_034329520.2:150..1532"
                     /db_xref="GeneID:117606711"
ORIGIN      
        1 makdaffsvi cckkltdier viknmksmvs dffypnmggv eehifnlsqc llgfghkvvv
       61 lthsygdrig vrymtnglkv yyipvkvfyn qcvlptmics ipliryifir eeiqivhghs
      121 afsalahegm ligrlmglkt tftdhslfgf adasailtnk fleislsdcn hcicvshtgk
      181 entvlranvl kekvsvipna vdttlfmpdi akrddnfiti vivsrlvyrk gvdllariip
      241 dicsrydnvq fliagdgpkr wlieevrern llqhrvtllg slehsqvrhv lnkghiflnt
      301 slteaycmai veaaacglqv vstkvggipe vlpedliylv eptvqalieg lefaikdyre
      361 gntkcpfemn krislfynwf nitkrteivy dlvkkeskmn lgeqlanyvr sgvlayllvv
      421 slcyiilqil eivvprkyid iakdyieinv iqsqgerked
//
LOCUS       XP_076545814             457 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class A isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_076545814
VERSION     XP_076545814.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689699.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class A isoform X2"
                     /calculated_mol_wt=51792
     Region          28..412
                     /region_name="GT4_PIG-A-like"
                     /note="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit A and similar
                     proteins; cd03796"
                     /db_xref="CDD:340827"
     Site            order(39,220..222,284,306)
                     /site_type="other"
                     /note="putative ADP-binding pocket [chemical binding]"
                     /db_xref="CDD:340827"
     CDS             1..457
                     /gene="PIG-A"
                     /coded_by="XM_076689699.1:155..1528"
                     /db_xref="GeneID:117606711"
ORIGIN      
        1 makdaffsvi cckkltdier viknmksmvs dffypnmggv eehifnlsqc llgfghkvvv
       61 lthsygdrig vrymtnglkv yyipvkvfyn qcvlptmics ipliryifir eeiqivhghs
      121 afsalahegm ligrlmglkt tftdhslfgf adasailtnk fleislsdcn hcicvshtgk
      181 entvlranvl kekvsvipna vdttlfmpdi akrddnfiiv srlvyrkgvd llariipdic
      241 srydnvqfli agdgpkrwli eevrernllq hrvtllgsle hsqvrhvlnk ghiflntslt
      301 eaycmaivea aacglqvvst kvggipevlp edliylvept vqalieglef aikdyregnt
      361 kcpfemnkri slfynwfnit krteivydlv kkeskmnlge qlanyvrsgv layllvvslc
      421 yiilqileiv vprkyidiak dyieinviqs qgerked
//
LOCUS       XP_034185413             442 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class A isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034185413
VERSION     XP_034185413.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329522.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..442
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class A isoform X3"
                     /calculated_mol_wt=50046
     Region          8..397
                     /region_name="GT4_PIG-A-like"
                     /note="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit A and similar
                     proteins; cd03796"
                     /db_xref="CDD:340827"
     Site            order(21,205..207,269,291)
                     /site_type="other"
                     /note="putative ADP-binding pocket [chemical binding]"
                     /db_xref="CDD:340827"
     CDS             1..442
                     /gene="PIG-A"
                     /coded_by="XM_034329522.2:431..1759"
                     /db_xref="GeneID:117606711"
ORIGIN      
        1 mstlkhkicm vsdffypnmg gveehifnls qcllgfghkv vvlthsygdr igvrymtngl
       61 kvyyipvkvf ynqcvlptmi csipliryif ireeiqivhg hsafsalahe gmligrlmgl
      121 kttftdhslf gfadasailt nkfleislsd cnhcicvsht gkentvlran vlkekvsvip
      181 navdttlfmp diakrddnfi tivivsrlvy rkgvdllari ipdicsrydn vqfliagdgp
      241 krwlieevre rnllqhrvtl lgslehsqvr hvlnkghifl ntslteaycm aiveaaacgl
      301 qvvstkvggi pevlpedliy lveptvqali eglefaikdy regntkcpfe mnkrislfyn
      361 wfnitkrtei vydlvkkesk mnlgeqlany vrsgvlayll vvslcyiilq ileivvprky
      421 idiakdyiei nviqsqgerk ed
//
LOCUS       XP_034185414             433 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class A isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034185414
VERSION     XP_034185414.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329523.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class A isoform X4"
                     /calculated_mol_wt=49003
     Region          1..388
                     /region_name="GT4_PIG-A-like"
                     /note="phosphatidylinositol
                     N-acetylglucosaminyltransferase subunit A and similar
                     proteins; cd03796"
                     /db_xref="CDD:340827"
     Site            order(12,196..198,260,282)
                     /site_type="other"
                     /note="putative ADP-binding pocket [chemical binding]"
                     /db_xref="CDD:340827"
     CDS             1..433
                     /gene="PIG-A"
                     /coded_by="XM_034329523.2:421..1722"
                     /db_xref="GeneID:117606711"
ORIGIN      
        1 mvsdffypnm ggveehifnl sqcllgfghk vvvlthsygd rigvrymtng lkvyyipvkv
       61 fynqcvlptm icsipliryi fireeiqivh ghsafsalah egmligrlmg lkttftdhsl
      121 fgfadasail tnkfleisls dcnhcicvsh tgkentvlra nvlkekvsvi pnavdttlfm
      181 pdiakrddnf itivivsrlv yrkgvdllar iipdicsryd nvqfliagdg pkrwlieevr
      241 ernllqhrvt llgslehsqv rhvlnkghif lntslteayc maiveaaacg lqvvstkvgg
      301 ipevlpedli ylveptvqal ieglefaikd yregntkcpf emnkrislfy nwfnitkrte
      361 ivydlvkkes kmnlgeqlan yvrsgvlayl lvvslcyiil qileivvprk yidiakdyie
      421 inviqsqger ked
//
LOCUS       XP_034185415             414 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 37A [Osmia lignaria lignaria].
ACCESSION   XP_034185415
VERSION     XP_034185415.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329524.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="vacuolar protein sorting 37A"
                     /calculated_mol_wt=47393
     Region          <53..116
                     /region_name="UEV_TSG101-like"
                     /note="ubiquitin E2 variant (UEV) domain of TSG101 and
                     related proteins; cd11685"
                     /db_xref="CDD:467408"
     Region          254..399
                     /region_name="Mod_r"
                     /note="Modifier of rudimentary (Mod(r)) protein;
                     pfam07200"
                     /db_xref="CDD:462117"
     CDS             1..414
                     /gene="Vsp37A"
                     /coded_by="XM_034329524.2:55..1299"
                     /db_xref="GeneID:117606712"
ORIGIN      
        1 misrifrgen envavkrkrq idtlkifndn vvelredvey qvqfnagdrr maivvslspe
       61 fpmekpvlrv sppishpwcn ehsevtsapg llnftvhsdl grvvqaiire fsknppqlle
      121 dvspvkshrd lqgrnspsys lqqypevpts ynsyyttqyp qyssvsants iynynhtnsg
      181 tsyvtdshtk fgspsqhsay iatsrsssih ntdpsrynst ylnshysnan yrqqslpsqa
      241 qtkprqsvaf pelngltnee lkrlsedddr ldefldnhsn lkdiniaide aidwvqktae
      301 anvakepelr elqsdvankv qtvtvlkary dqliqrynkl sevftpdhik eclkkaades
      361 heesekiaed flnrkidver flstyiecrk lgqarrtkee klahqlnelk ragy
//
LOCUS       XP_034185416             346 aa            linear   INV 03-SEP-2025
DEFINITION  guanine nucleotide-binding protein subunit beta-2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185416
VERSION     XP_034185416.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329525.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="guanine nucleotide-binding protein subunit
                     beta-2"
                     /calculated_mol_wt=37753
     Region          55..345
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(58,76,80,86..87,99..100,118,122,128..129,147..148,
                     165,169,175..176,189,206,211,217..218,230..231,249,253,
                     259..260,274..275,292,297,303..304,316..317,335,339,345)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          64..99
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          105..147
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          152..187
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          195..229
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          235..271
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          279..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          321..345
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..346
                     /gene="Gbeta76C"
                     /coded_by="XM_034329525.2:157..1197"
                     /db_xref="GeneID:117606713"
ORIGIN      
        1 mgkddaetva lkkeledlin kckedqkkqq dttleeacsg aadapkvkls tkkllkghin
       61 kvnsvhysgd srhcvtgsld gkliiwdswt gnkvqviplr sawvmsvafa psgnfvacgg
      121 mdnmctiydv nnrdatgsak ivrellgyeg flsscrffed kkiitgsgdm kiciwdlean
      181 kkttdfcgha gdvvsislsp dgntyvtgsv drtcklwdvr eetahqtffg headvnsvcf
      241 hpsgqafvta sedktarlwd lrsdqqlatf kppsatpgyt scglsfsgrf ifcgsddnti
      301 hiwdtlknqh ngvlsghenr vtslsvagng maiascswdq nvriwv
//
LOCUS       XP_034185417             160 aa            linear   INV 03-SEP-2025
DEFINITION  immunoglobulin domain-containing protein oig-4-like isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034185417
VERSION     XP_034185417.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329526.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..160
                     /product="immunoglobulin domain-containing protein
                     oig-4-like isoform X1"
                     /calculated_mol_wt=18331
     Region          73..142
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..160
                     /gene="LOC117606714"
                     /coded_by="XM_034329526.2:191..673"
                     /db_xref="GeneID:117606714"
ORIGIN      
        1 msrsmvifll ivlllnsqet larrgrargk srsrvqiglp itgkyrdpes dqyynnhnga
       61 killsshfdl eyvlghkivf lcvargnprp hitwfkdgae iynhlylhvh ewqegpdqvk
      121 skleidpatq mdagvyecta dnmysidrrs fktdfsiafd
//
LOCUS       XP_034185418             274 aa            linear   INV 03-SEP-2025
DEFINITION  putative oxidoreductase PXDNL isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185418
VERSION     XP_034185418.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329527.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..274
                     /product="putative oxidoreductase PXDNL isoform X2"
                     /calculated_mol_wt=31271
     Region          79..>113
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          82..86
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          95..99
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          187..256
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..274
                     /gene="LOC117606714"
                     /coded_by="XM_034329527.2:1..825"
                     /db_xref="GeneID:117606714"
ORIGIN      
        1 mtrcrllylf lftalligtd aakgggargg grrgggrvyk srmpiliphr npasanyyen
       61 kdgarivkss hfeldymlgr kitffcmatg fprpeitwlk dgielyhhkf fqivmsrsmv
      121 ifllivllln sqetlarrgr argksrsrvq iglpitgkyr dpesdqyynn hngakillss
      181 hfdleyvlgh kivflcvarg nprphitwfk dgaeiynhly lhvhewqegp dqvkskleid
      241 patqmdagvy ectadnmysi drrsfktdfs iafd
//
LOCUS       XP_034185419             127 aa            linear   INV 03-SEP-2025
DEFINITION  transcription initiation factor TFIID subunit 10 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185419
VERSION     XP_034185419.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329528.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..127
                     /product="transcription initiation factor TFIID subunit
                     10"
                     /calculated_mol_wt=14118
     Region          24..118
                     /region_name="HFD_TAF10"
                     /note="histone-fold domain found in transcription
                     initiation factor TFIID subunit 10 (TAF10) and similar
                     proteins; cd07982"
                     /db_xref="CDD:467026"
     Site            order(25..26,29..30,32..33,36..41,44..45,47..49,52..55,58,
                     60..61,64..65,67..74,76..78,80..81,84,101..106,109,113,
                     116)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467026"
     Site            order(47,50..55,58,60,72,82,86..87)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467026"
     CDS             1..127
                     /gene="LOC117606715"
                     /coded_by="XM_034329528.2:240..623"
                     /db_xref="GeneID:117606715"
ORIGIN      
        1 maedfgrdtp pmytgneetk tagqplsdfl lqledytptv pdaisehylh tagfnttdpr
       61 ivrlvslaaq kfiseianda lqhcktrgan qntktkgkdr rytltmedlt pavaeygiiv
      121 kkphyfv
//
LOCUS       XP_076545815             106 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611352 [Osmia lignaria lignaria].
ACCESSION   XP_076545815
VERSION     XP_076545815.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689700.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="uncharacterized protein LOC117611352"
                     /calculated_mol_wt=11684
     CDS             1..106
                     /gene="LOC117611352"
                     /coded_by="XM_076689700.1:622..942"
                     /db_xref="GeneID:117611352"
ORIGIN      
        1 mkvnqgkrsr rptrrggvkq raralikrqi qlvqkeletr fppsgpsshp gepcyspvse
       61 asticlgprs pqlidlteep elesvnvaet neipaeilai ifnlqe
//
LOCUS       XP_034190246             862 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 7 member 14 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190246
VERSION     XP_034190246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..862
                     /product="solute carrier family 7 member 14 isoform X1"
                     /calculated_mol_wt=93647
     Region          104..765
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..862
                     /gene="LOC117608770"
                     /coded_by="XM_034334355.2:1..2589"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mqhcifprfs lflitntnln knllpevcln cgwlsvkdyq alkfsvikkf rqinekmkfe
       61 lpmdrekgle lfgkfirtkn veslqgeqrk ggpeplhptd pkqklqkclt tldltslgvg
      121 scvgtgmylv agmvarsvag pgvvfsfiia aiasifsgac yaefgvrvph ttgsaymysy
      181 vtvgeliafi igwnmvleyl igtsacacal sacldalaeg avsgaiansv gtifgrppdf
      241 lafvitllmm llmaagvkks lvfnnvlnai nlavwvfvmt agmfyvdsan wrehngflpy
      301 gwsgvftgaa tcfyafigfd iiattgeeat npkrsiplai vssliiilva yvtssmvltl
      361 ivpydevdqd salvemfgqv gaykckyiva vgalagltvs mfgsmfpmpr ivyamaqdgl
      421 ifrslsqvwp atgtpalatl tsglcaalaa lliqlevlve mmsigtllay tlvstcvlil
      481 ryqphstnlv ellppslrtp crsptkenqg ngqvnygkel rpdqlttaln tvqaaqselt
      541 aansgqrimv rrvrrcnsss pdsddtygae edevglgkdd qylvsdrten kfygsvhaaa
      601 aasscgsthq ypgntpiigp plnylqrrlq aaqylcpaif pwvdrgpate asgryvmklv
      661 gllyllivif dlivvcgmgn mgtfttiimf aflfaiigvl laisrkpqnr nsmmfmtpgl
      721 pfvpaiavtv niylifklsi ltlvrftvwm vlgfimyfyy gikhssleea saaetqeenv
      781 tagnieltvt dpqrqpqthp spytttdhsi yegqqldafg qpvfgstnfg gtpnqrqett
      841 tassatgpal fvahesfpsw dd
//
LOCUS       XP_034190247             842 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 7 member 14 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190247
VERSION     XP_034190247.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334356.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..842
                     /product="solute carrier family 7 member 14 isoform X2"
                     /calculated_mol_wt=91405
     Region          104..745
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..842
                     /gene="LOC117608770"
                     /coded_by="XM_034334356.2:1..2529"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mqhcifprfs lflitntnln knllpevcln cgwlsvkdyq alkfsvikkf rqinekmkfe
       61 lpmdrekgle lfgkfirtkn veslqgeqrk ggpeplhptd pkqklqkclt tldltslgvg
      121 scvgtgmylv agmvarsvag pgvvfsfiia aiasifsgac yaefgvrvph ttgsaymysy
      181 vtvgeliafi igwnmvleyl igtsacacal sacldalaeg avsgaiansv gtifgrppdf
      241 lafvitllmm llmaagvkks lvfnnvlnai nlavwvfvmt agmfyvdsan wrehngflpy
      301 gwsgvftgaa tcfyafigfd iiattgeeat npkrsiplai vssliiilva yvtssmvltl
      361 ivpydevdqd salvemfgqv gaykckyiva vgalagltvs mfgsmfpmpr ivyamaqdgl
      421 ifrslsqvwp atgtpalatl tsglcaalaa lliqlevlve mmsigtllay tlvstcvlil
      481 ryqphstnlv ellppslrtp crsptkenqg ngqaaqselt aansgqrimv rrvrrcnsss
      541 pdsddtygae edevglgkdd qylvsdrten kfygsvhaaa aasscgsthq ypgntpiigp
      601 plnylqrrlq aaqylcpaif pwvdrgpate asgryvmklv gllyllivif dlivvcgmgn
      661 mgtfttiimf aflfaiigvl laisrkpqnr nsmmfmtpgl pfvpaiavtv niylifklsi
      721 ltlvrftvwm vlgfimyfyy gikhssleea saaetqeenv tagnieltvt dpqrqpqthp
      781 spytttdhsi yegqqldafg qpvfgstnfg gtpnqrqett tassatgpal fvahesfpsw
      841 dd
//
LOCUS       XP_034190249             806 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 7 member 14 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190249
VERSION     XP_034190249.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334358.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..806
                     /product="solute carrier family 7 member 14 isoform X3"
                     /calculated_mol_wt=87019
     Region          48..709
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..806
                     /gene="LOC117608770"
                     /coded_by="XM_034334358.2:793..3213"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mkfelpmdre kglelfgkfi rtknveslqg eqrkggpepl hptdpkqklq kclttldlts
       61 lgvgscvgtg mylvagmvar svagpgvvfs fiiaaiasif sgacyaefgv rvphttgsay
      121 mysyvtvgel iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr
      181 ppdflafvit llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng
      241 flpygwsgvf tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm
      301 vltlivpyde vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama
      361 qdglifrsls qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc
      421 vlilryqphs tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq
      481 seltaansgq rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv
      541 haaaaasscg sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv
      601 mklvgllyll ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm
      661 tpglpfvpai avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq
      721 eenvtagnie ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr
      781 qetttassat gpalfvahes fpswdd
//
LOCUS       XP_034190250             736 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190250
VERSION     XP_034190250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334359.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="putative cationic amino acid transporter isoform
                     X4"
                     /calculated_mol_wt=79322
     Region          1..639
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..736
                     /gene="LOC117608770"
                     /coded_by="XM_034334359.2:737..2947"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mylvagmvar svagpgvvfs fiiaaiasif sgacyaefgv rvphttgsay mysyvtvgel
       61 iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr ppdflafvit
      121 llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng flpygwsgvf
      181 tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm vltlivpyde
      241 vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama qdglifrsls
      301 qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc vlilryqphs
      361 tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq seltaansgq
      421 rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv haaaaasscg
      481 sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv mklvgllyll
      541 ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm tpglpfvpai
      601 avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq eenvtagnie
      661 ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr qetttassat
      721 gpalfvahes fpswdd
//
LOCUS       XP_034190252             736 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190252
VERSION     XP_034190252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334361.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="putative cationic amino acid transporter isoform
                     X4"
                     /calculated_mol_wt=79322
     Region          1..639
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..736
                     /gene="LOC117608770"
                     /coded_by="XM_034334361.2:1159..3369"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mylvagmvar svagpgvvfs fiiaaiasif sgacyaefgv rvphttgsay mysyvtvgel
       61 iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr ppdflafvit
      121 llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng flpygwsgvf
      181 tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm vltlivpyde
      241 vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama qdglifrsls
      301 qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc vlilryqphs
      361 tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq seltaansgq
      421 rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv haaaaasscg
      481 sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv mklvgllyll
      541 ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm tpglpfvpai
      601 avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq eenvtagnie
      661 ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr qetttassat
      721 gpalfvahes fpswdd
//
LOCUS       XP_034190251             736 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190251
VERSION     XP_034190251.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334360.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="putative cationic amino acid transporter isoform
                     X4"
                     /calculated_mol_wt=79322
     Region          1..639
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..736
                     /gene="LOC117608770"
                     /coded_by="XM_034334360.2:459..2669"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mylvagmvar svagpgvvfs fiiaaiasif sgacyaefgv rvphttgsay mysyvtvgel
       61 iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr ppdflafvit
      121 llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng flpygwsgvf
      181 tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm vltlivpyde
      241 vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama qdglifrsls
      301 qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc vlilryqphs
      361 tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq seltaansgq
      421 rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv haaaaasscg
      481 sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv mklvgllyll
      541 ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm tpglpfvpai
      601 avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq eenvtagnie
      661 ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr qetttassat
      721 gpalfvahes fpswdd
//
LOCUS       XP_076545816             686 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545816
VERSION     XP_076545816.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689701.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..686
                     /product="putative cationic amino acid transporter isoform
                     X5"
                     /calculated_mol_wt=74226
     Region          1..589
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..686
                     /gene="LOC117608770"
                     /coded_by="XM_076689701.1:394..2454"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mysyvtvgel iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr
       61 ppdflafvit llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng
      121 flpygwsgvf tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm
      181 vltlivpyde vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama
      241 qdglifrsls qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc
      301 vlilryqphs tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq
      361 seltaansgq rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv
      421 haaaaasscg sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv
      481 mklvgllyll ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm
      541 tpglpfvpai avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq
      601 eenvtagnie ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr
      661 qetttassat gpalfvahes fpswdd
//
LOCUS       XP_076545817             686 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545817
VERSION     XP_076545817.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689702.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..686
                     /product="putative cationic amino acid transporter isoform
                     X5"
                     /calculated_mol_wt=74226
     Region          1..589
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..686
                     /gene="LOC117608770"
                     /coded_by="XM_076689702.1:290..2350"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mysyvtvgel iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr
       61 ppdflafvit llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng
      121 flpygwsgvf tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm
      181 vltlivpyde vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama
      241 qdglifrsls qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc
      301 vlilryqphs tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq
      361 seltaansgq rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv
      421 haaaaasscg sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv
      481 mklvgllyll ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm
      541 tpglpfvpai avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq
      601 eenvtagnie ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr
      661 qetttassat gpalfvahes fpswdd
//
LOCUS       XP_076545818             686 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545818
VERSION     XP_076545818.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689703.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..686
                     /product="putative cationic amino acid transporter isoform
                     X5"
                     /calculated_mol_wt=74226
     Region          1..589
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..686
                     /gene="LOC117608770"
                     /coded_by="XM_076689703.1:133..2193"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mysyvtvgel iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr
       61 ppdflafvit llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng
      121 flpygwsgvf tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm
      181 vltlivpyde vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama
      241 qdglifrsls qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc
      301 vlilryqphs tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq
      361 seltaansgq rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv
      421 haaaaasscg sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv
      481 mklvgllyll ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm
      541 tpglpfvpai avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq
      601 eenvtagnie ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr
      661 qetttassat gpalfvahes fpswdd
//
LOCUS       XP_076545819             686 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545819
VERSION     XP_076545819.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689704.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..686
                     /product="putative cationic amino acid transporter isoform
                     X5"
                     /calculated_mol_wt=74226
     Region          1..589
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..686
                     /gene="LOC117608770"
                     /coded_by="XM_076689704.1:359..2419"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mysyvtvgel iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr
       61 ppdflafvit llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng
      121 flpygwsgvf tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm
      181 vltlivpyde vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama
      241 qdglifrsls qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc
      301 vlilryqphs tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq
      361 seltaansgq rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv
      421 haaaaasscg sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv
      481 mklvgllyll ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm
      541 tpglpfvpai avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq
      601 eenvtagnie ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr
      661 qetttassat gpalfvahes fpswdd
//
LOCUS       XP_076545820             686 aa            linear   INV 03-SEP-2025
DEFINITION  putative cationic amino acid transporter isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545820
VERSION     XP_076545820.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689705.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..686
                     /product="putative cationic amino acid transporter isoform
                     X5"
                     /calculated_mol_wt=74226
     Region          1..589
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..686
                     /gene="LOC117608770"
                     /coded_by="XM_076689705.1:153..2213"
                     /db_xref="GeneID:117608770"
ORIGIN      
        1 mysyvtvgel iafiigwnmv leyligtsac acalsaclda laegavsgai ansvgtifgr
       61 ppdflafvit llmmllmaag vkkslvfnnv lnainlavwv fvmtagmfyv dsanwrehng
      121 flpygwsgvf tgaatcfyaf igfdiiattg eeatnpkrsi plaivsslii ilvayvtssm
      181 vltlivpyde vdqdsalvem fgqvgaykck yivavgalag ltvsmfgsmf pmprivyama
      241 qdglifrsls qvwpatgtpa latltsglca alaalliqle vlvemmsigt llaytlvstc
      301 vlilryqphs tnlvellpps lrtpcrsptk enqgngqvny gkelrpdqlt talntvqaaq
      361 seltaansgq rimvrrvrrc nssspdsddt ygaeedevgl gkddqylvsd rtenkfygsv
      421 haaaaasscg sthqypgntp iigpplnylq rrlqaaqylc paifpwvdrg pateasgryv
      481 mklvgllyll ivifdlivvc gmgnmgtftt iimfaflfai igvllaisrk pqnrnsmmfm
      541 tpglpfvpai avtvniylif klsiltlvrf tvwmvlgfim yfyygikhss leeasaaetq
      601 eenvtagnie ltvtdpqrqp qthpspyttt dhsiyegqql dafgqpvfgs tnfggtpnqr
      661 qetttassat gpalfvahes fpswdd
//
LOCUS       XP_034190601             669 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase OSR1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190601
VERSION     XP_034190601.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334710.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..669
                     /product="serine/threonine-protein kinase OSR1 isoform X1"
                     /calculated_mol_wt=73418
     Region          180..456
                     /region_name="STKc_OSR1_SPAK"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Oxidative stress response kinase and Ste20-related proline
                     alanine-rich kinase; cd06610"
                     /db_xref="CDD:270787"
     Site            order(188..192,196,209,211,241,257..260,263..264,267,311,
                     313..316,318,328..329,332,351..354,356,384,393)
                     /site_type="active"
                     /db_xref="CDD:270787"
     Site            order(188..192,196,209,211,241,257..260,263..264,267,313,
                     315..316,318,329)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270787"
     Site            order(191..192,311,313..315,332,351..354,356,384,393)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270787"
     Site            order(312..313,337..339,352..365,367..369,373,376..377,
                     380..381,384,390,397,400..402,404..405,439,441..442,444)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270787"
     Site            order(328..341,348..356)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270787"
     Region          562..632
                     /region_name="OSR1_C"
                     /note="Oxidative-stress-responsive kinase 1 C-terminal
                     domain; pfam12202"
                     /db_xref="CDD:463491"
     CDS             1..669
                     /gene="LOC117608941"
                     /coded_by="XM_034334710.2:64..2073"
                     /db_xref="GeneID:117608941"
ORIGIN      
        1 mrcervnaid maadlyangs vkstsgriag smrqrktgtl gaaapistke erlkfqdnrf
       61 lttfvlgrnr kiapdvlpsc spgifrqksf rassppksas avtknpaatn nrdracadlt
      121 sprdlktsns ssrskssgvl llargtrgts kharlgtvla nkmassssnt vvqgwpntkd
      181 dyelkevigv gatavvhaaf ciprqekcai krinlekwnt smdellkeiq amsscnhenv
      241 vtyytsfvvk eelwlvlrll eggslldiik hktrttnckh gvfdeatiat vlrevlkgle
      301 yfhsngqihr dikagnillg edgtvqiadf gvsawlatgr dlsrqkvrht fvgtpcwmap
      361 evmeqdhgyd fkadiwslgi taiemasgta pyhkyppmkv lmltlqndpp tldtaaddkd
      421 qykaygktfr kmivdclqkd ptkrptatel lkhpffkkak dkkylqqtlv aigpsletrv
      481 qkaskrqpgt sgrlhrtvtg ewvwsseeed sdassadegk ealpvntien psseeeeeev
      541 yglvksvpsk lvvqatdqnv pinlvlrlrr peshftleen sinqkrelnd irfefavgvd
      601 saegiaaelv gaglvdgkdi vviaanlqkl idsagqlrtv tfslnsghaa nevpddkali
      661 gfaqisitd
//
LOCUS       XP_034190602             656 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase OSR1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190602
VERSION     XP_034190602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..656
                     /product="serine/threonine-protein kinase OSR1 isoform X2"
                     /calculated_mol_wt=71877
     Region          180..456
                     /region_name="STKc_OSR1_SPAK"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Oxidative stress response kinase and Ste20-related proline
                     alanine-rich kinase; cd06610"
                     /db_xref="CDD:270787"
     Site            order(188..192,196,209,211,241,257..260,263..264,267,311,
                     313..316,318,328..329,332,351..354,356,384,393)
                     /site_type="active"
                     /db_xref="CDD:270787"
     Site            order(188..192,196,209,211,241,257..260,263..264,267,313,
                     315..316,318,329)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270787"
     Site            order(191..192,311,313..315,332,351..354,356,384,393)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270787"
     Site            order(312..313,337..339,352..365,367..369,373,376..377,
                     380..381,384,390,397,400..402,404..405,439,441..442,444)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270787"
     Site            order(328..341,348..356)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270787"
     Region          562..619
                     /region_name="OSR1_C"
                     /note="Oxidative-stress-responsive kinase 1 C-terminal
                     domain; pfam12202"
                     /db_xref="CDD:463491"
     CDS             1..656
                     /gene="LOC117608941"
                     /coded_by="XM_034334711.2:64..2034"
                     /db_xref="GeneID:117608941"
ORIGIN      
        1 mrcervnaid maadlyangs vkstsgriag smrqrktgtl gaaapistke erlkfqdnrf
       61 lttfvlgrnr kiapdvlpsc spgifrqksf rassppksas avtknpaatn nrdracadlt
      121 sprdlktsns ssrskssgvl llargtrgts kharlgtvla nkmassssnt vvqgwpntkd
      181 dyelkevigv gatavvhaaf ciprqekcai krinlekwnt smdellkeiq amsscnhenv
      241 vtyytsfvvk eelwlvlrll eggslldiik hktrttnckh gvfdeatiat vlrevlkgle
      301 yfhsngqihr dikagnillg edgtvqiadf gvsawlatgr dlsrqkvrht fvgtpcwmap
      361 evmeqdhgyd fkadiwslgi taiemasgta pyhkyppmkv lmltlqndpp tldtaaddkd
      421 qykaygktfr kmivdclqkd ptkrptatel lkhpffkkak dkkylqqtlv aigpsletrv
      481 qkaskrqpgt sgrlhrtvtg ewvwsseeed sdassadegk ealpvntien psseeeeeev
      541 yglvksvpsk lvvqatdqnv pinlvlrlrn qkrelndirf efavgvdsae giaaelvgag
      601 lvdgkdivvi aanlqklids agqlrtvtfs lnsghaanev pddkaligfa qisitd
//
LOCUS       XP_076545821             655 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase OSR1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545821
VERSION     XP_076545821.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689706.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..655
                     /product="serine/threonine-protein kinase OSR1 isoform X3"
                     /calculated_mol_wt=72233
     Region          163..442
                     /region_name="STKc_OSR1_SPAK"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Oxidative stress response kinase and Ste20-related proline
                     alanine-rich kinase; cd06610"
                     /db_xref="CDD:270787"
     Site            order(171..175,179,192,194,224,240..243,246..247,250,294,
                     296..299,301,311..312,315,334..337,339,370,379)
                     /site_type="active"
                     /db_xref="CDD:270787"
     Site            order(171..175,179,192,194,224,240..243,246..247,250,296,
                     298..299,301,312)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270787"
     Site            order(174..175,294,296..298,315,334..337,339,370,379)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270787"
     Site            order(295..296,320..322,335..348,353..355,359,362..363,
                     366..367,370,376,383,386..388,390..391,425,427..428,430)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270787"
     Site            order(311..324,331..339)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270787"
     Region          548..618
                     /region_name="OSR1_C"
                     /note="Oxidative-stress-responsive kinase 1 C-terminal
                     domain; pfam12202"
                     /db_xref="CDD:463491"
     CDS             1..655
                     /gene="LOC117608941"
                     /coded_by="XM_076689706.1:153..2120"
                     /db_xref="GeneID:117608941"
ORIGIN      
        1 mirflefykt iagsmrqrkt gtlgaaapis tkeerlkfqd nrflttfvlg rnrkiapdvl
       61 pscspgifrq ksfrassppk sasavtknpa atnnrdraca dltsprdlkt snsssrskss
      121 gvlllargtr gtskharlgt vlankmasss sntvvqgwpn tkddyelkev igvgatavvh
      181 aafciprqek caikrinlek wntsmdellk eiqamsscnh envvtyytsf vvkeelwlvl
      241 rlleggslld iikhktrttn ckhgvfdeat iatvlrevlk gleyfhsngq ihrdikagni
      301 llgedgtvqi adfgvsawla tgrdlsrqkv rhtfvgtpcw mapevmeqvr edhgydfkad
      361 iwslgitaie masgtapyhk yppmkvlmlt lqndpptldt aaddkdqyka ygktfrkmiv
      421 dclqkdptkr ptatellkhp ffkkakdkky lqqtlvaigp sletrvqkas krqpgtsgrl
      481 hrtvtgewvw sseeedsdas sadegkealp vntienpsse eeeeevyglv ksvpsklvvq
      541 atdqnvpinl vlrlrrpesh ftleensinq krelndirfe favgvdsaeg iaaelvgagl
      601 vdgkdivvia anlqklidsa gqlrtvtfsl nsghaanevp ddkaligfaq isitd
//
LOCUS       XP_076545823             652 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase OSR1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545823
VERSION     XP_076545823.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689708.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..652
                     /product="serine/threonine-protein kinase OSR1 isoform X4"
                     /calculated_mol_wt=71753
     Region          160..439
                     /region_name="STKc_OSR1_SPAK"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Oxidative stress response kinase and Ste20-related proline
                     alanine-rich kinase; cd06610"
                     /db_xref="CDD:270787"
     Site            order(168..172,176,189,191,221,237..240,243..244,247,291,
                     293..296,298,308..309,312,331..334,336,367,376)
                     /site_type="active"
                     /db_xref="CDD:270787"
     Site            order(168..172,176,189,191,221,237..240,243..244,247,293,
                     295..296,298,309)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270787"
     Site            order(171..172,291,293..295,312,331..334,336,367,376)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270787"
     Site            order(292..293,317..319,332..345,350..352,356,359..360,
                     363..364,367,373,380,383..385,387..388,422,424..425,427)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270787"
     Site            order(308..321,328..336)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270787"
     Region          545..615
                     /region_name="OSR1_C"
                     /note="Oxidative-stress-responsive kinase 1 C-terminal
                     domain; pfam12202"
                     /db_xref="CDD:463491"
     CDS             1..652
                     /gene="LOC117608941"
                     /coded_by="XM_076689708.1:155..2113"
                     /db_xref="GeneID:117608941"
ORIGIN      
        1 mclllfqiag smrqrktgtl gaaapistke erlkfqdnrf lttfvlgrnr kiapdvlpsc
       61 spgifrqksf rassppksas avtknpaatn nrdracadlt sprdlktsns ssrskssgvl
      121 llargtrgts kharlgtvla nkmassssnt vvqgwpntkd dyelkevigv gatavvhaaf
      181 ciprqekcai krinlekwnt smdellkeiq amsscnhenv vtyytsfvvk eelwlvlrll
      241 eggslldiik hktrttnckh gvfdeatiat vlrevlkgle yfhsngqihr dikagnillg
      301 edgtvqiadf gvsawlatgr dlsrqkvrht fvgtpcwmap evmeqvredh gydfkadiws
      361 lgitaiemas gtapyhkypp mkvlmltlqn dpptldtaad dkdqykaygk tfrkmivdcl
      421 qkdptkrpta tellkhpffk kakdkkylqq tlvaigpsle trvqkaskrq pgtsgrlhrt
      481 vtgewvwsse eedsdassad egkealpvnt ienpsseeee eevyglvksv psklvvqatd
      541 qnvpinlvlr lrrpeshftl eensinqkre lndirfefav gvdsaegiaa elvgaglvdg
      601 kdivviaanl qklidsagql rtvtfslnsg haanevpddk aligfaqisi td
//
LOCUS       XP_034190606             654 aa            linear   INV 03-SEP-2025
DEFINITION  dihydrouridine synthase 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190606
VERSION     XP_034190606.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334715.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190606.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..654
                     /product="dihydrouridine synthase 3 isoform X1"
                     /calculated_mol_wt=74936
     Region          308..539
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(312..314,338,367,395,437,467,499,501,524..525)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(367,398..399,437,439,466..467,469..470,500..501,525)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(398,439,467,469)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(399,437,466,470,500..501)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     CDS             1..654
                     /gene="Dus3"
                     /coded_by="XM_034334715.2:43..2007"
                     /db_xref="GeneID:117608942"
ORIGIN      
        1 mfllekvakl aeitndrsvs iryimmaeys ekctedcsfd ysedkqkgvc vikeeyiigd
       61 hirllseecl tktdkekvst sttnsdedvs hepplkklkg ddkreklrgq nkarpppfka
      121 qrelnlcpwi adqavgepek kcdnkrctfl hdrvqylkik padigdecyl fnitgrcprg
      181 aacrmgskhl ledglnivdk ekeeefkklp pstknqltkh lqiqlrkhky nfskaekvvk
      241 anepsrkkle sakqieqssk vtepqirnds ndknsendtt eqklgpveds dlikcrnvek
      301 kkidwedkil lsplttvgnl pfrrickeyg aditcgemal aprilkgahe ewalvkrhes
      361 edifgvqlcg nnpgvltrca qllnqeidvd fidlnlgcpi dliyrqgggs gmlnrlnvle
      421 tvvksvnqim dipltiksrt gvyidkpvah nlmpkfsewg vsmitvhgrs reqrytklad
      481 wdyiekcakv aqpvpvfgng dilsfddyqr vrdtytsvsg itigrgalik pwifteikek
      541 klidissner feilkkytny glehwgsdtr gvettrrfml ewisflhryv pvgilerppq
      601 rinerppfyr grdemetlma ssncadwikl selllgkvpe gfrflpkhka nswk
//
LOCUS       XP_076545824             651 aa            linear   INV 03-SEP-2025
DEFINITION  dihydrouridine synthase 3 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545824
VERSION     XP_076545824.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689709.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..651
                     /product="dihydrouridine synthase 3 isoform X2"
                     /calculated_mol_wt=74575
     Region          305..536
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(309..311,335,364,392,434,464,496,498,521..522)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(364,395..396,434,436,463..464,466..467,497..498,522)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(395,436,464,466)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(396,434,463,467,497..498)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     CDS             1..651
                     /gene="Dus3"
                     /coded_by="XM_076689709.1:16..1971"
                     /db_xref="GeneID:117608942"
ORIGIN      
        1 mlrvaklaei tndrsvsiry immaeysekc tedcsfdyse dkqkgvcvik eeyiigdhir
       61 llseecltkt dkekvststt nsdedvshep plkklkgddk reklrgqnka rpppfkaqre
      121 lnlcpwiadq avgepekkcd nkrctflhdr vqylkikpad igdecylfni tgrcprgaac
      181 rmgskhlled glnivdkeke eefkklppst knqltkhlqi qlrkhkynfs kaekvvkane
      241 psrkklesak qieqsskvte pqirndsndk nsendtteqk lgpvedsdli kcrnvekkki
      301 dwedkillsp lttvgnlpfr rickeygadi tcgemalapr ilkgaheewa lvkrhesedi
      361 fgvqlcgnnp gvltrcaqll nqeidvdfid lnlgcpidli yrqgggsgml nrlnvletvv
      421 ksvnqimdip ltiksrtgvy idkpvahnlm pkfsewgvsm itvhgrsreq rytkladwdy
      481 iekcakvaqp vpvfgngdil sfddyqrvrd tytsvsgiti grgalikpwi fteikekkli
      541 dissnerfei lkkytnygle hwgsdtrgve ttrrfmlewi sflhryvpvg ilerppqrin
      601 erppfyrgrd emetlmassn cadwiklsel llgkvpegfr flpkhkansw k
//
LOCUS       XP_034190607             613 aa            linear   INV 03-SEP-2025
DEFINITION  sodium-coupled monocarboxylate transporter 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190607
VERSION     XP_034190607.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334716.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190607.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..613
                     /product="sodium-coupled monocarboxylate transporter 1"
                     /calculated_mol_wt=66762
     Region          43..562
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(93,96,378,381..382)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..613
                     /gene="LOC117608943"
                     /coded_by="XM_034334716.2:203..2044"
                     /db_xref="GeneID:117608943"
ORIGIN      
        1 mrsrilvlfv flavssaeqr psdsaeekcl anhptflhyf swpdytvlaa mlliscligt
       61 fygffakkqe tsqdfllggs smgtfpmams laasfitaie llgnpaemye qgtqfwmtcv
      121 afilvvpits ylylpvymkl rltssyeyln lrfdrhcrll asslymlqmi lytsvavyap
      181 alalshvtgl ntyiavtlvy vvcifyasqg gmkavimtdt fqaavllgsl flivgyglsw
      241 eggissvwka neesgrmeff nlnpnptirh sfwsvaiggt vywtsmfcsn qasvqkylsv
      301 esigqartal wvsavgmiii ytvnfltgmv lystykdcdp llagyisgqd qllplyvmnf
      361 mgglngvpgl fvagifaasl gtvasalnsl aaitcedifq glfkielpar kgaiyarwis
      421 iffgvlsfal vfvverlgsv lqvalsfngm vggitlglfs lgmfvpwana kgaisgaits
      481 liivlwiglg aqiavlngqi hldskpvstv acpcmsnttt ivpihphdtg ddgiffiyki
      541 sylwysaigs iltilvgalv sfatgfqnpa didqdllspp iaslfnmqtk prtnnvqgit
      601 nfalelddek ivk
//
LOCUS       XP_034190608             596 aa            linear   INV 03-SEP-2025
DEFINITION  putative sodium-dependent multivitamin transporter [Osmia lignaria
            lignaria].
ACCESSION   XP_034190608
VERSION     XP_034190608.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334717.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190608.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="putative sodium-dependent multivitamin
                     transporter"
                     /calculated_mol_wt=65618
     Region          9..523
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(58,61,343,346..347)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..596
                     /gene="LOC117608944"
                     /coded_by="XM_034334717.2:863..2653"
                     /db_xref="GeneID:117608944"
ORIGIN      
        1 mektlqwedy lvivatlcis igigiyyrfs ggrqktmeey fvasrsmsim pvgvalvvsf
       61 msaitllgvs aenytygtqf vvinlsylig tplvcygflp vffklqatsa yeylekrfgi
      121 kartmasfvy wlqlllysgv vlyapalale attgisktgs iiiiglvcaf yssiggikav
      181 litdvfqgll mfvsvfiiiv taaneaggld kiweiaqagn riefdsisid ptvrhtwwsl
      241 nigglftfls lygvnqvqvq rlltvrdika aqkalwlawp ilsilsittc fsglaiysky
      301 yncdpllqhk isstdmlmpy yvmdtmsdkp glaglfiagi fsaglstisa alnslaavtl
      361 edylkpvyrk ccakefsatt sttlakilaf lygilsiala flaqllggvl qagltifgvv
      421 ggpllglftl gmltetasei gsvtgatisl allfwtafgq prptpptlpt sdagctnati
      481 lssetqfsqt sndssyfyly risymwycpl gfvltfvigl llsvcinrmf kkpkveldtn
      541 lffpmvgeri rrrreriles seddvpkdnt aqrkyvftik ptndqenmde ynitrv
//
LOCUS       XP_034190609             596 aa            linear   INV 03-SEP-2025
DEFINITION  putative sodium-dependent multivitamin transporter [Osmia lignaria
            lignaria].
ACCESSION   XP_034190609
VERSION     XP_034190609.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334718.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190609.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="putative sodium-dependent multivitamin
                     transporter"
                     /calculated_mol_wt=65618
     Region          9..523
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(58,61,343,346..347)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..596
                     /gene="LOC117608944"
                     /coded_by="XM_034334718.2:737..2527"
                     /db_xref="GeneID:117608944"
ORIGIN      
        1 mektlqwedy lvivatlcis igigiyyrfs ggrqktmeey fvasrsmsim pvgvalvvsf
       61 msaitllgvs aenytygtqf vvinlsylig tplvcygflp vffklqatsa yeylekrfgi
      121 kartmasfvy wlqlllysgv vlyapalale attgisktgs iiiiglvcaf yssiggikav
      181 litdvfqgll mfvsvfiiiv taaneaggld kiweiaqagn riefdsisid ptvrhtwwsl
      241 nigglftfls lygvnqvqvq rlltvrdika aqkalwlawp ilsilsittc fsglaiysky
      301 yncdpllqhk isstdmlmpy yvmdtmsdkp glaglfiagi fsaglstisa alnslaavtl
      361 edylkpvyrk ccakefsatt sttlakilaf lygilsiala flaqllggvl qagltifgvv
      421 ggpllglftl gmltetasei gsvtgatisl allfwtafgq prptpptlpt sdagctnati
      481 lssetqfsqt sndssyfyly risymwycpl gfvltfvigl llsvcinrmf kkpkveldtn
      541 lffpmvgeri rrrreriles seddvpkdnt aqrkyvftik ptndqenmde ynitrv
//
LOCUS       XP_034190610             596 aa            linear   INV 03-SEP-2025
DEFINITION  putative sodium-dependent multivitamin transporter [Osmia lignaria
            lignaria].
ACCESSION   XP_034190610
VERSION     XP_034190610.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334719.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190610.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="putative sodium-dependent multivitamin
                     transporter"
                     /calculated_mol_wt=65618
     Region          9..523
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(58,61,343,346..347)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..596
                     /gene="LOC117608944"
                     /coded_by="XM_034334719.2:672..2462"
                     /db_xref="GeneID:117608944"
ORIGIN      
        1 mektlqwedy lvivatlcis igigiyyrfs ggrqktmeey fvasrsmsim pvgvalvvsf
       61 msaitllgvs aenytygtqf vvinlsylig tplvcygflp vffklqatsa yeylekrfgi
      121 kartmasfvy wlqlllysgv vlyapalale attgisktgs iiiiglvcaf yssiggikav
      181 litdvfqgll mfvsvfiiiv taaneaggld kiweiaqagn riefdsisid ptvrhtwwsl
      241 nigglftfls lygvnqvqvq rlltvrdika aqkalwlawp ilsilsittc fsglaiysky
      301 yncdpllqhk isstdmlmpy yvmdtmsdkp glaglfiagi fsaglstisa alnslaavtl
      361 edylkpvyrk ccakefsatt sttlakilaf lygilsiala flaqllggvl qagltifgvv
      421 ggpllglftl gmltetasei gsvtgatisl allfwtafgq prptpptlpt sdagctnati
      481 lssetqfsqt sndssyfyly risymwycpl gfvltfvigl llsvcinrmf kkpkveldtn
      541 lffpmvgeri rrrreriles seddvpkdnt aqrkyvftik ptndqenmde ynitrv
//
LOCUS       XP_034190611             596 aa            linear   INV 03-SEP-2025
DEFINITION  putative sodium-dependent multivitamin transporter [Osmia lignaria
            lignaria].
ACCESSION   XP_034190611
VERSION     XP_034190611.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334720.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190611.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="putative sodium-dependent multivitamin
                     transporter"
                     /calculated_mol_wt=65618
     Region          9..523
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(58,61,343,346..347)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..596
                     /gene="LOC117608944"
                     /coded_by="XM_034334720.2:126..1916"
                     /db_xref="GeneID:117608944"
ORIGIN      
        1 mektlqwedy lvivatlcis igigiyyrfs ggrqktmeey fvasrsmsim pvgvalvvsf
       61 msaitllgvs aenytygtqf vvinlsylig tplvcygflp vffklqatsa yeylekrfgi
      121 kartmasfvy wlqlllysgv vlyapalale attgisktgs iiiiglvcaf yssiggikav
      181 litdvfqgll mfvsvfiiiv taaneaggld kiweiaqagn riefdsisid ptvrhtwwsl
      241 nigglftfls lygvnqvqvq rlltvrdika aqkalwlawp ilsilsittc fsglaiysky
      301 yncdpllqhk isstdmlmpy yvmdtmsdkp glaglfiagi fsaglstisa alnslaavtl
      361 edylkpvyrk ccakefsatt sttlakilaf lygilsiala flaqllggvl qagltifgvv
      421 ggpllglftl gmltetasei gsvtgatisl allfwtafgq prptpptlpt sdagctnati
      481 lssetqfsqt sndssyfyly risymwycpl gfvltfvigl llsvcinrmf kkpkveldtn
      541 lffpmvgeri rrrreriles seddvpkdnt aqrkyvftik ptndqenmde ynitrv
//
LOCUS       XP_034190614             463 aa            linear   INV 03-SEP-2025
DEFINITION  pigment-dispersing factor receptor [Osmia lignaria lignaria].
ACCESSION   XP_034190614
VERSION     XP_034190614.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334723.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190614.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..463
                     /product="pigment-dispersing factor receptor"
                     /calculated_mol_wt=53065
     Region          31..97
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          126..397
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          126..150
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320389"
     Region          159..180
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320389"
     Region          205..227
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320389"
     Region          246..262
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320389"
     Region          285..308
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320389"
     Region          333..355
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320389"
     Region          363..388
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320389"
     CDS             1..463
                     /gene="Pdfr"
                     /coded_by="XM_034334723.2:1019..2410"
                     /db_xref="GeneID:117608945"
ORIGIN      
        1 mdasnaignn trdfcvarsk nfvppddelw ckpvwdsllc wpptkastti kqrcpfedgf
       61 dttksvekkc gyngrwegqn gtshedshhg wanyttclip emlrlhgkvy tntiegnmkm
      121 eivektrile fvglsislva llaslaifcr frslrntrtr ihknlfvamv vqvlirltvy
      181 idieiqrkkt ygiqrgignt pvlceasyvl leyaktsmfm wmfieglflh nmvtvtvfqe
      241 tsyyrmyrfi gwgcpvvmtl iwaiitafyy hpksrfsrcw sgynlssyfw ilegprfavi
      301 llnflfllni vrvlvvklrq shtseieqvl kavraavvll pllgitnvlc midaplhnvk
      361 kfavwsysty flqsfqglfi atlycflnge vrlaldktis vylslrgtdl qprrqstfsg
      421 cqpqrvasvi emeeeemrps swirlccrgg ntpppdrpae tav
//
LOCUS       XP_034190622             367 aa            linear   INV 03-SEP-2025
DEFINITION  stromal cell derived factor mayday [Osmia lignaria lignaria].
ACCESSION   XP_034190622
VERSION     XP_034190622.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334731.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..367
                     /product="stromal cell derived factor mayday"
                     /calculated_mol_wt=42850
     Region          93..352
                     /region_name="EFh_CREC"
                     /note="EF-hand, calcium binding motif, found in CREC-EF
                     hand family; cl25354"
                     /db_xref="CDD:330175"
     Region          107..136
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320021"
     Region          146..176
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320021"
     Region          203..232
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320021"
     Region          240..269
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320021"
     Region          287..316
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320021"
     Region          323..352
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320021"
     CDS             1..367
                     /gene="myd"
                     /coded_by="XM_034334731.2:471..1574"
                     /db_xref="GeneID:117608948"
ORIGIN      
        1 mylsyfinki tcksdlrcfr flrwtilvpl iiylfllfak ynrnvplksl vspatakdee
       61 gsmiedlfve levvtaprdk eldrrrevdr sikeveeaen renprslled ifqradtdqd
      121 qlldiqelar wihmkitehi scamrenigl ftaidnnpqn gevsweeyha yflrshgfpe
      181 syvsshdkkh sdmsrtlkes imrdrarwae aarndperla ldeflafthp esshrallqm
      241 vedlfekfdr dgdeqltede fsdlpaegvg ldlkedrhea vggsedrrke frhlidknkn
      301 gkadraellm yidprnprha iqeaqhlinl sdtnldgkln lseilskmdl flgskmvdte
      361 ksfhdef
//
LOCUS       XP_034190626             208 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608950 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190626
VERSION     XP_034190626.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334735.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190626.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="uncharacterized protein LOC117608950 isoform X1"
                     /calculated_mol_wt=24468
     CDS             1..208
                     /gene="LOC117608950"
                     /coded_by="XM_034334735.2:31..657"
                     /db_xref="GeneID:117608950"
ORIGIN      
        1 mnegntfykt penesfsttv geehpsktdg tgrhllaama vlgdcsynvy kkcnrsylqm
       61 reinrlhlpl aqdmaylpee dyieervrke eqsfdrnnrn llpeyrslce tvtkkvqldd
      121 seyeyqpphy heiycksysl idnierpvnf skqkcvepsy hcvqrsrrll lmrrrwdgec
      181 wepfskqias gcdcmwpvsv lgditvhy
//
LOCUS       XP_034190628             190 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608950 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190628
VERSION     XP_034190628.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334737.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190628.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..190
                     /product="uncharacterized protein LOC117608950 isoform X2"
                     /calculated_mol_wt=22633
     CDS             1..190
                     /gene="LOC117608950"
                     /coded_by="XM_034334737.2:551..1123"
                     /db_xref="GeneID:117608950"
ORIGIN      
        1 maskelstyr yqrssrtlaa mavlgdcsyn vykkcnrsyl qmreinrlhl plaqdmaylp
       61 eedyieervr keeqsfdrnn rnllpeyrsl cetvtkkvql ddseyeyqpp hyheiycksy
      121 slidnierpv nfskqkcvep syhcvqrsrr lllmrrrwdg ecwepfskqi asgcdcmwpv
      181 svlgditvhy
//
LOCUS       XP_034190630             182 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608950 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190630
VERSION     XP_034190630.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334739.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190630.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..182
                     /product="uncharacterized protein LOC117608950 isoform X3"
                     /calculated_mol_wt=21654
     CDS             1..182
                     /gene="LOC117608950"
                     /coded_by="XM_034334739.2:242..790"
                     /db_xref="GeneID:117608950"
ORIGIN      
        1 myrlgylgll aamavlgdcs ynvykkcnrs ylqmreinrl hlplaqdmay lpeedyieer
       61 vrkeeqsfdr nnrnllpeyr slcetvtkkv qlddseyeyq pphyheiyck syslidnier
      121 pvnfskqkcv epsyhcvqrs rrlllmrrrw dgecwepfsk qiasgcdcmw pvsvlgditv
      181 hy
//
LOCUS       XP_076545825             453 aa            linear   INV 03-SEP-2025
DEFINITION  trace amine-associated receptor 1 [Osmia lignaria lignaria].
ACCESSION   XP_076545825
VERSION     XP_076545825.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689710.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="trace amine-associated receptor 1"
                     /calculated_mol_wt=50580
     Region          90..364
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          112..138
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(134,137..138,150..155,157..158,161,211,213..217,240,
                     243..245,247..249,251..252,320,323..324,326..327,330,
                     338..339,341..343,346,349..350)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          150..180
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410626"
     Region          193..217
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410626"
     Region          240..265
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          296..330
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     Region          339..364
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..453
                     /gene="LOC117610861"
                     /coded_by="XM_076689710.1:606..1967"
                     /db_xref="GeneID:117610861"
ORIGIN      
        1 mksrrdlrvs aqprarherl vwgrrsdtcf ntrhanqeem ehkqtillpp alsnsscwqr
       61 dtseaiwtvp gpaellraal illlslgilc anllvicvin skryskyiha qprylltsla
      121 sndfaigllv tpfgflpavy gcwpygevvc qiqallrgal tqqsavilic maidryvcml
      181 hphhyhkhas kkyymrqgcv tvmsttwias maifgalvlp rggyyfnnsg llacdpffar
      241 aslrilaccc fyfpttmvlm ycygsafhvn klrlkrvvcv ntaevvnggh idrlmaqerr
      301 lstaasrtma amslgfivmv tpwtiqevva actgskpppf ldflatwlal snsfwnpfly
      361 wllnnhfrri srellytkif ckakslgpkq hccatstggl dvcsmvgvdl sglercsidr
      421 ysmarcssvs lvnapaalpw eightlhses ppt
//
LOCUS       XP_076545826             446 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611102 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545826
VERSION     XP_076545826.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689711.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="uncharacterized protein LOC117611102 isoform X1"
                     /calculated_mol_wt=52351
     Region          48..392
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     Region          <234..395
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..446
                     /gene="LOC117611102"
                     /coded_by="XM_076689711.1:386..1726"
                     /db_xref="GeneID:117611102"
ORIGIN      
        1 merkqgqtir searniirhi irkcdeesrk nemtyllpqa tlrasyytgv svkticrirk
       61 edaecdenep lptpgakrpr gedkkfycsl edqatirsii ydfyvtkkim ptgpklltai
      121 rqlidfpwel hslyrllrrm gfewrkssns ikrvlverps ivacrskylk aieyyrkqnr
      181 piiyidetwv ddlcfhkclh sketlemvnt tssserlvvv hagwkegfik gaelifkthi
      241 ttgdpeglmt aenfekwive kllpnippks ivvmdnvpyh saqlnkpptk yskkadmvrw
      301 lvenniphtq dmrkyqllel iekskkcrkt fkvdemlklh nhnalrlppy myyelnpidl
      361 awtqikrklr eknlfvlssd dletftveai nevtqidwqn fcehieklee eywqkdglve
      421 ditdslelqp ggsddsdsde stdfed
//
LOCUS       XP_034194909             446 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611102 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194909
VERSION     XP_034194909.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339018.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194909.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="uncharacterized protein LOC117611102 isoform X1"
                     /calculated_mol_wt=52351
     Region          48..392
                     /region_name="transpos_IS630"
                     /note="IS630 family transposase; NF033545"
                     /db_xref="CDD:468076"
     Region          <234..395
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..446
                     /gene="LOC117611102"
                     /coded_by="XM_034339018.2:386..1726"
                     /db_xref="GeneID:117611102"
ORIGIN      
        1 merkqgqtir searniirhi irkcdeesrk nemtyllpqa tlrasyytgv svkticrirk
       61 edaecdenep lptpgakrpr gedkkfycsl edqatirsii ydfyvtkkim ptgpklltai
      121 rqlidfpwel hslyrllrrm gfewrkssns ikrvlverps ivacrskylk aieyyrkqnr
      181 piiyidetwv ddlcfhkclh sketlemvnt tssserlvvv hagwkegfik gaelifkthi
      241 ttgdpeglmt aenfekwive kllpnippks ivvmdnvpyh saqlnkpptk yskkadmvrw
      301 lvenniphtq dmrkyqllel iekskkcrkt fkvdemlklh nhnalrlppy myyelnpidl
      361 awtqikrklr eknlfvlssd dletftveai nevtqidwqn fcehieklee eywqkdglve
      421 ditdslelqp ggsddsdsde stdfed
//
LOCUS       XP_076545827             140 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611102 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545827
VERSION     XP_076545827.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689712.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="uncharacterized protein LOC117611102 isoform X2"
                     /calculated_mol_wt=16116
     CDS             1..140
                     /gene="LOC117611102"
                     /coded_by="XM_076689712.1:297..719"
                     /db_xref="GeneID:117611102"
ORIGIN      
        1 myiisvytyi stcdsavlmw kiervkyiny tkvqravtkc ltwswyiadn mqffvfgifv
       61 lilsssyyyt alslsiatif lsmlvhayit ydtgyiptle ftricssenh srlshrgpig
      121 kyfislavfl ylpipyvpsg
//
LOCUS       XP_076545828             123 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611102 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545828
VERSION     XP_076545828.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689713.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="uncharacterized protein LOC117611102 isoform X3"
                     /calculated_mol_wt=14203
     CDS             1..123
                     /gene="LOC117611102"
                     /coded_by="XM_076689713.1:297..668"
                     /db_xref="GeneID:117611102"
ORIGIN      
        1 myiisvytyi stcdsavlmw kiervkyiny tkvqravtkc ltwswyiadn mqffvfgifv
       61 lilsssyyyt alslsiatif lsmlvhayit ydtgyiptle ftricssenh srlshrgpig
      121 rsl
//
LOCUS       XP_076545829             102 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611102 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545829
VERSION     XP_076545829.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689714.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="uncharacterized protein LOC117611102 isoform X4"
                     /calculated_mol_wt=11586
     CDS             1..102
                     /gene="LOC117611102"
                     /coded_by="XM_076689714.1:1337..1645"
                     /db_xref="GeneID:117611102"
ORIGIN      
        1 mcltwswyia dnmqffvfgi fvlilsssyy ytalslsiat iflsmlvhay itydtgyipt
       61 leftricsse nhsrlshrgp igkyfislav flylpipyvp sg
//
LOCUS       XP_034194573             184 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610853 [Osmia lignaria lignaria].
ACCESSION   XP_034194573
VERSION     XP_034194573.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338682.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..184
                     /product="uncharacterized protein LOC117610853"
                     /calculated_mol_wt=21294
     CDS             1..184
                     /gene="LOC117610853"
                     /coded_by="XM_034338682.2:837..1391"
                     /db_xref="GeneID:117610853"
ORIGIN      
        1 mvdtytkdcr mmlvenreaa tlipiikenv iasseihtdc crrvrvrvss rttwsvsnkk
       61 ksirkvrsif akddhyksvq kseggtfrye vqtanrialq tdrnrpvdls eykstgtvts
      121 pnkkrprmnv lektskdail girrkvhefw frreiprmkk mleqtgkkfn kastklfipn
      181 pget
//
LOCUS       XP_034185519             574 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box, sub-group O isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185519
VERSION     XP_034185519.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329628.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..574
                     /product="forkhead box, sub-group O isoform X1"
                     /calculated_mol_wt=62241
     Region          112..191
                     /region_name="FH_FOXO"
                     /note="Forkhead (FH) domain found in the Forkhead box
                     protein O (FOXO) subfamily; cd20032"
                     /db_xref="CDD:410806"
     Region          114..>339
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Site            order(115..116,134,138,162..163,165..166,169,176,185..186,
                     188)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410806"
     Region          482..516
                     /region_name="FOXO-TAD"
                     /note="Transactivation domain of FOXO protein family;
                     pfam16676"
                     /db_xref="CDD:435506"
     CDS             1..574
                     /gene="foxo"
                     /coded_by="XM_034329628.2:1128..2852"
                     /db_xref="GeneID:117606754"
ORIGIN      
        1 mlavkveyte etnmmdsvpv saagsktglq pqqgvpydle sgfepqtrar shtwplprpe
       61 eyiegnvvps vpaskegveg vesgtpsqhg slgqgtgllp vkknssrrna wgnhsyadli
      121 tqaitsapde rltlsqiyew mmqnipyfre kgesnssagw knsirhnlsl hsrfmrvqne
      181 gtgksswwmi nrdakpgkss rrraitmets kfekrrgrar kkiealrngg lqadatpsps
      241 nsvnegldlf pdsplqpgsg fqlspdfrpr assnasscgr lspipsipgk pewtspytps
      301 yspeqlagsl aeamklesyq myhhtsppnh tqqtgpppsy yetqyqrsns lttgsssfal
      361 qptpqsanqq rcpihglqpc acqmnlspva gmspsyqqse psptalgnsq qqtlqymmqt
      421 qqcqqsqqqq qqqqqqqqqq qqsqtegtgs sppqtptpcg ptpstmmgql mgalnnssll
      481 ddlniniesl hggfdcnvee vikhelsmdg tldfnfqqgv mgtaaiqvsd snvgqngvvt
      541 qnnvvvgtta gnapagvyvs snaatpaapp swvh
//
LOCUS       XP_076545830             400 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box, sub-group O isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545830
VERSION     XP_076545830.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689715.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="forkhead box, sub-group O isoform X2"
                     /calculated_mol_wt=43058
     Region          <1..19
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Region          308..342
                     /region_name="FOXO-TAD"
                     /note="Transactivation domain of FOXO protein family;
                     pfam16676"
                     /db_xref="CDD:435506"
     CDS             1..400
                     /gene="foxo"
                     /coded_by="XM_076689715.1:172..1374"
                     /db_xref="GeneID:117606754"
ORIGIN      
        1 mrvqnegtgk sswwminrda kpgkssrrra itmetskfek rrgrarkkie alrngglqad
       61 atpspsnsvn egldlfpdsp lqpgsgfqls pdfrprassn asscgrlspi psipgkpewt
      121 spytpsyspe qlagslaeam klesyqmyhh tsppnhtqqt gpppsyyetq yqrsnslttg
      181 sssfalqptp qsanqqrcpi hglqpcacqm nlspvagmsp syqqsepspt algnsqqqtl
      241 qymmqtqqcq qsqqqqqqqq qqqqqqqqsq tegtgssppq tptpcgptps tmmgqlmgal
      301 nnssllddln inieslhggf dcnveevikh elsmdgtldf nfqqgvmgta aiqvsdsnvg
      361 qngvvtqnnv vvgttagnap agvyvssnaa tpaappswvh
//
LOCUS       XP_076545831             400 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box, sub-group O isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545831
VERSION     XP_076545831.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689716.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="forkhead box, sub-group O isoform X2"
                     /calculated_mol_wt=43058
     Region          <1..19
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Region          308..342
                     /region_name="FOXO-TAD"
                     /note="Transactivation domain of FOXO protein family;
                     pfam16676"
                     /db_xref="CDD:435506"
     CDS             1..400
                     /gene="foxo"
                     /coded_by="XM_076689716.1:160..1362"
                     /db_xref="GeneID:117606754"
ORIGIN      
        1 mrvqnegtgk sswwminrda kpgkssrrra itmetskfek rrgrarkkie alrngglqad
       61 atpspsnsvn egldlfpdsp lqpgsgfqls pdfrprassn asscgrlspi psipgkpewt
      121 spytpsyspe qlagslaeam klesyqmyhh tsppnhtqqt gpppsyyetq yqrsnslttg
      181 sssfalqptp qsanqqrcpi hglqpcacqm nlspvagmsp syqqsepspt algnsqqqtl
      241 qymmqtqqcq qsqqqqqqqq qqqqqqqqsq tegtgssppq tptpcgptps tmmgqlmgal
      301 nnssllddln inieslhggf dcnveevikh elsmdgtldf nfqqgvmgta aiqvsdsnvg
      361 qngvvtqnnv vvgttagnap agvyvssnaa tpaappswvh
//
LOCUS       XP_076545832             400 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box, sub-group O isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545832
VERSION     XP_076545832.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689717.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="forkhead box, sub-group O isoform X2"
                     /calculated_mol_wt=43058
     Region          <1..19
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Region          308..342
                     /region_name="FOXO-TAD"
                     /note="Transactivation domain of FOXO protein family;
                     pfam16676"
                     /db_xref="CDD:435506"
     CDS             1..400
                     /gene="foxo"
                     /coded_by="XM_076689717.1:117..1319"
                     /db_xref="GeneID:117606754"
ORIGIN      
        1 mrvqnegtgk sswwminrda kpgkssrrra itmetskfek rrgrarkkie alrngglqad
       61 atpspsnsvn egldlfpdsp lqpgsgfqls pdfrprassn asscgrlspi psipgkpewt
      121 spytpsyspe qlagslaeam klesyqmyhh tsppnhtqqt gpppsyyetq yqrsnslttg
      181 sssfalqptp qsanqqrcpi hglqpcacqm nlspvagmsp syqqsepspt algnsqqqtl
      241 qymmqtqqcq qsqqqqqqqq qqqqqqqqsq tegtgssppq tptpcgptps tmmgqlmgal
      301 nnssllddln inieslhggf dcnveevikh elsmdgtldf nfqqgvmgta aiqvsdsnvg
      361 qngvvtqnnv vvgttagnap agvyvssnaa tpaappswvh
//
LOCUS       XP_076545833             356 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305578 [Osmia lignaria lignaria].
ACCESSION   XP_076545833
VERSION     XP_076545833.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689718.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..356
                     /product="uncharacterized protein LOC143305578"
                     /calculated_mol_wt=39902
     CDS             1..356
                     /gene="LOC143305578"
                     /coded_by="XM_076689718.1:161..1231"
                     /db_xref="GeneID:143305578"
ORIGIN      
        1 mkvkycvqvf sqsvgsvmtl msrnkvksvc gtremplegv dtarfihfmn difdsvnssr
       61 plsvvdince rsniwdvgin vfrsmtfikr kfadkerplv vhnwirtlqn fkflttllgn
      121 lgfshfigrn fnqdavenff gqirqhgarn tnpscttfhg yyrtlllnsy sqlvsadtnc
      181 eseesanfli slkkylspap aasmidnsif lnynvsaaat vltvvqyvsr rvlkknttck
      241 fclalvfein vsegssmhva crslitqvvs iclanmpqia htpnliatlk lcisehvnfk
      301 ltcsehgnsl ttyvihqcii yvvknyigrv nrvingseep assdplitra lhvsrk
//
LOCUS       XP_076545834             104 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305580 [Osmia lignaria lignaria].
ACCESSION   XP_076545834
VERSION     XP_076545834.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689719.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..104
                     /product="uncharacterized protein LOC143305580"
                     /calculated_mol_wt=11134
     CDS             1..104
                     /gene="LOC143305580"
                     /coded_by="XM_076689719.1:120..434"
                     /db_xref="GeneID:143305580"
ORIGIN      
        1 mwkrklqanr twrtllpstc glrassnsvq cseftdptvt savsgclers gpklrlsvnd
       61 slpskqvhsw mngkndaair dnavgegrga arkvqgaarv apri
//
LOCUS       XP_076545835             440 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305581 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545835
VERSION     XP_076545835.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689720.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..440
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305581"
                     /calculated_mol_wt=50129
     CDS             1..440
                     /gene="LOC143305581"
                     /coded_by="XM_076689720.1:1..1323"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:143305581"
ORIGIN      
        1 mrllgigrdg inlfcglmdm fsglskhaye rivehirdss kiiflklskk aveeekrene
       61 kreihlnnlk vsgdgswkkr gftslygvtt liayhigkvi dlvvkssych gctfyknken
      121 tqeyqdwive henectanhe gsagkmevda vvemfqrsee cfgvryqnyi gdgdsktfkr
      181 ilnvnlygdk fqvtknecig hvkkpmgtrl rnvkkeqklg gkgkltnkli kdltnyygla
      241 ikrnensveg mkksimatyy hlcstnenpr yencptgeds xckwevaqaq ntnynphfil
      301 msknistiyk dlskedlltr clgghsqnpn esynsticrf apkhlhsrle iveisaylaa
      361 yisnegfsav llimqtldli igrqsrdlai krdedrlkrq erqslnatke arlacrqhql
      421 eenalyeete gilygpgiad
//
LOCUS       XP_034185520             547 aa            linear   INV 03-SEP-2025
DEFINITION  chaperonin containing TCP1 subunit 7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185520
VERSION     XP_034185520.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329629.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185520.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..547
                     /product="chaperonin containing TCP1 subunit 7 isoform X1"
                     /calculated_mol_wt=60314
     Region          9..528
                     /region_name="TCP1_eta"
                     /note="TCP-1 (CTT or eukaryotic type II) chaperonin
                     family, eta subunit. Chaperonins are involved in
                     productive folding of proteins. They share a common
                     general morphology, a double toroid of 2 stacked rings. In
                     contrast to bacterial group I chaperonins (GroEL);
                     cd03340"
                     /db_xref="CDD:239456"
     Site            order(17,21,49..52,54,72,74,78,82,85,383,519..525)
                     /site_type="other"
                     /note="ring oligomerisation interface [polypeptide
                     binding]"
                     /db_xref="CDD:239456"
     Site            order(44..46,96,100,165,395,413,453,496,498)
                     /site_type="other"
                     /note="ATP/Mg binding site [chemical binding]"
                     /db_xref="CDD:239456"
     Site            order(433,451,460,462..463,466)
                     /site_type="active"
                     /note="stacking interactions [active]"
                     /db_xref="CDD:239456"
     CDS             1..547
                     /gene="CCT7"
                     /coded_by="XM_034329629.2:122..1765"
                     /db_xref="GeneID:117606755"
ORIGIN      
        1 mgkymrqpqi illkegtdal qgkqqlisni nacqvvvdav rttlgprgmd klivdqngka
       61 tisndgatil kllevihpaa ktlvdiaksq daevgdgtts vvllageflk qmkpfieegv
      121 hprimikalr ialqvavnki navsvkidks skeklmelle ecaatsmssk lihqqkkffs
      181 rmvvkavmml ddmlplnmig ikkvsggale dselvkgvaf kktfsyagfe mqpkkyqdck
      241 iallnielel kaerdnaeir vdnvaeyqki vdaewqilyn kldkihqsga qvvlsklpig
      301 dvatqyfadr dmfcagrvpe edlrrtlkac ggnilttvhd ikdsdlgrce mfeerqigge
      361 rfnffyegsr aktctfilrg gaeqfleete rslhdaimvv rrlfktnayv agggaiemel
      421 sktlrdysri vagkeqllig aiaralevip rqlcdnagfd atsilnklrq kqhkgmhtyg
      481 vdintedigd nmaafvwepa vvkinaltaa seaaclilsv detiknpkcg qdngpqapmg
      541 rgmgrpm
//
LOCUS       XP_034185521             542 aa            linear   INV 03-SEP-2025
DEFINITION  chaperonin containing TCP1 subunit 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185521
VERSION     XP_034185521.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329630.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185521.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="chaperonin containing TCP1 subunit 7 isoform X2"
                     /calculated_mol_wt=59679
     Region          4..523
                     /region_name="TCP1_eta"
                     /note="TCP-1 (CTT or eukaryotic type II) chaperonin
                     family, eta subunit. Chaperonins are involved in
                     productive folding of proteins. They share a common
                     general morphology, a double toroid of 2 stacked rings. In
                     contrast to bacterial group I chaperonins (GroEL);
                     cd03340"
                     /db_xref="CDD:239456"
     Site            order(12,16,44..47,49,67,69,73,77,80,378,514..520)
                     /site_type="other"
                     /note="ring oligomerisation interface [polypeptide
                     binding]"
                     /db_xref="CDD:239456"
     Site            order(39..41,91,95,160,390,408,448,491,493)
                     /site_type="other"
                     /note="ATP/Mg binding site [chemical binding]"
                     /db_xref="CDD:239456"
     Site            order(428,446,455,457..458,461)
                     /site_type="active"
                     /note="stacking interactions [active]"
                     /db_xref="CDD:239456"
     CDS             1..542
                     /gene="CCT7"
                     /coded_by="XM_034329630.2:156..1784"
                     /db_xref="GeneID:117606755"
ORIGIN      
        1 mqpqiillke gtdalqgkqq lisninacqv vvdavrttlg prgmdklivd qngkatisnd
       61 gatilkllev ihpaaktlvd iaksqdaevg dgttsvvlla geflkqmkpf ieegvhprim
      121 ikalrialqv avnkinavsv kidksskekl melleecaat smssklihqq kkffsrmvvk
      181 avmmlddmlp lnmigikkvs ggaledselv kgvafkktfs yagfemqpkk yqdckialln
      241 ielelkaerd naeirvdnva eyqkivdaew qilynkldki hqsgaqvvls klpigdvatq
      301 yfadrdmfca grvpeedlrr tlkacggnil ttvhdikdsd lgrcemfeer qiggerfnff
      361 yegsraktct filrggaeqf leeterslhd aimvvrrlfk tnayvaggga iemelsktlr
      421 dysrivagke qlligaiara leviprqlcd nagfdatsil nklrqkqhkg mhtygvdint
      481 edigdnmaaf vwepavvkin altaaseaac lilsvdetik npkcgqdngp qapmgrgmgr
      541 pm
//
LOCUS       XP_076545836             530 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606762 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545836
VERSION     XP_076545836.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689721.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..530
                     /product="uncharacterized protein LOC117606762 isoform X1"
                     /calculated_mol_wt=59067
     Region          2..54
                     /region_name="SH3_CD2AP-like_1"
                     /note="First Src Homology 3 domain (SH3A) of
                     CD2-associated protein and similar proteins; cd11873"
                     /db_xref="CDD:212806"
     Site            order(7,9,12..13,15..16,32..35,46,48,50..51)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212806"
     Region          94..144
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(98,100,103,107,125..126,139,141..142)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212807"
     Region          163..216
                     /region_name="SH3_CD2AP-like_3"
                     /note="Third Src Homology 3 domain (SH3C) of
                     CD2-associated protein and similar proteins; cd11875"
                     /db_xref="CDD:212808"
     Site            order(166..173,175..176,180..181,194..197,207..208,210,
                     212..213,215)
                     /site_type="other"
                     /note="ubiquitin interaction site [polypeptide binding]"
                     /db_xref="CDD:212808"
     Site            order(167,169,172,176,195..197,208,210,212..213)
                     /site_type="other"
                     /note="putative peptide ligand binding site [polypeptide
                     binding]"
                     /db_xref="CDD:212808"
     CDS             1..530
                     /gene="LOC117606762"
                     /coded_by="XM_076689721.1:204..1796"
                     /db_xref="GeneID:117606762"
ORIGIN      
        1 meaiveynye aqepdeltlk kgdiiteiev ltggwwegtl rdkrgmfpdn fvkvlgpvss
       61 gtvssietaa segvnnnkse dvplrnggsr rrfckvlfdy epcnkdeltl ipqdtieflg
      121 evedgwwrgr lkgkvgvfps nfvtrpvyee pdkhkhqnkk elykvifpyk aanddeltln
      181 egdiitllsk easdegwwvg eingriglfp dnfvevvnva qdqtdheqrq etsvksaarh
      241 shvpkknekv erekvrrsld vqsvhaeink kaistsasss tsstspgige snedrktisy
      301 siisslkrfl gdvgtnngne ntnislgeel dvveriegap lshltasrak aprrrppssq
      361 rlrrytagpt itttttptpe dnlangngdt iskqlrekep eglaakarrk apwvtelkln
      421 hiekrkigvv dhvdiselkk kwnysrvspt tnvtkaikqk dtspksnass qpeqtspttg
      481 tpayvpydvf nqllervasl eekqamlqea lteileqfvv tislinnvtn
//
LOCUS       XP_076545837             510 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606762 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545837
VERSION     XP_076545837.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689722.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="uncharacterized protein LOC117606762 isoform X2"
                     /calculated_mol_wt=56758
     Region          2..54
                     /region_name="SH3_CD2AP-like_1"
                     /note="First Src Homology 3 domain (SH3A) of
                     CD2-associated protein and similar proteins; cd11873"
                     /db_xref="CDD:212806"
     Site            order(7,9,12..13,15..16,32..35,46,48,50..51)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212806"
     Region          94..144
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(98,100,103,107,125..126,139,141..142)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212807"
     Region          163..216
                     /region_name="SH3_CD2AP-like_3"
                     /note="Third Src Homology 3 domain (SH3C) of
                     CD2-associated protein and similar proteins; cd11875"
                     /db_xref="CDD:212808"
     Site            order(166..173,175..176,180..181,194..197,207..208,210,
                     212..213,215)
                     /site_type="other"
                     /note="ubiquitin interaction site [polypeptide binding]"
                     /db_xref="CDD:212808"
     Site            order(167,169,172,176,195..197,208,210,212..213)
                     /site_type="other"
                     /note="putative peptide ligand binding site [polypeptide
                     binding]"
                     /db_xref="CDD:212808"
     CDS             1..510
                     /gene="LOC117606762"
                     /coded_by="XM_076689722.1:204..1736"
                     /db_xref="GeneID:117606762"
ORIGIN      
        1 meaiveynye aqepdeltlk kgdiiteiev ltggwwegtl rdkrgmfpdn fvkvlgpvss
       61 gtvssietaa segvnnnkse dvplrnggsr rrfckvlfdy epcnkdeltl ipqdtieflg
      121 evedgwwrgr lkgkvgvfps nfvtrpvyee pdkhkhqnkk elykvifpyk aanddeltln
      181 egdiitllsk easdegwwvg eingriglfp dnfvevvnva qdqtdheqrq etsvksaarh
      241 shvpkknekv erekvrrsld vqsvhaeink kaistsasss tsstspgige sdvgtnngne
      301 ntnislgeel dvveriegap lshltasrak aprrrppssq rlrrytagpt itttttptpe
      361 dnlangngdt iskqlrekep eglaakarrk apwvtelkln hiekrkigvv dhvdiselkk
      421 kwnysrvspt tnvtkaikqk dtspksnass qpeqtspttg tpayvpydvf nqllervasl
      481 eekqamlqea lteileqfvv tislinnvtn
//
LOCUS       XP_076545838             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606762 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545838
VERSION     XP_076545838.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689723.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC117606762 isoform X3"
                     /calculated_mol_wt=54406
     Region          2..54
                     /region_name="SH3_CD2AP-like_1"
                     /note="First Src Homology 3 domain (SH3A) of
                     CD2-associated protein and similar proteins; cd11873"
                     /db_xref="CDD:212806"
     Site            order(7,9,12..13,15..16,32..35,46,48,50..51)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212806"
     Region          94..144
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(98,100,103,107,125..126,139,141..142)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212807"
     Region          163..216
                     /region_name="SH3_CD2AP-like_3"
                     /note="Third Src Homology 3 domain (SH3C) of
                     CD2-associated protein and similar proteins; cd11875"
                     /db_xref="CDD:212808"
     Site            order(166..173,175..176,180..181,194..197,207..208,210,
                     212..213,215)
                     /site_type="other"
                     /note="ubiquitin interaction site [polypeptide binding]"
                     /db_xref="CDD:212808"
     Site            order(167,169,172,176,195..197,208,210,212..213)
                     /site_type="other"
                     /note="putative peptide ligand binding site [polypeptide
                     binding]"
                     /db_xref="CDD:212808"
     CDS             1..486
                     /gene="LOC117606762"
                     /coded_by="XM_076689723.1:204..1664"
                     /db_xref="GeneID:117606762"
ORIGIN      
        1 meaiveynye aqepdeltlk kgdiiteiev ltggwwegtl rdkrgmfpdn fvkvlgpvss
       61 gtvssietaa segvnnnkse dvplrnggsr rrfckvlfdy epcnkdeltl ipqdtieflg
      121 evedgwwrgr lkgkvgvfps nfvtrpvyee pdkhkhqnkk elykvifpyk aanddeltln
      181 egdiitllsk easdegwwvg eingriglfp dnfvevvnva qdqtdheqrq etsvksaarh
      241 shvpkknekv erekvrrsld vqsvhagdvg tnngnentni slgeeldvve riegaplshl
      301 tasrakaprr rppssqrlrr ytagptittt ttptpednla ngngdtiskq lrekepegla
      361 akarrkapwv telklnhiek rkigvvdhvd iselkkkwny srvspttnvt kaikqkdtsp
      421 ksnassqpeq tspttgtpay vpydvfnqll ervasleekq amlqealtei leqfvvtisl
      481 innvtn
//
LOCUS       XP_076545839             412 aa            linear   INV 03-SEP-2025
DEFINITION  phospholipase B1, membrane-associated-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545839
VERSION     XP_076545839.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689724.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..412
                     /product="phospholipase B1, membrane-associated-like
                     isoform X1"
                     /calculated_mol_wt=46559
     Region          83..376
                     /region_name="Phospholipase_B_like"
                     /note="Phospholipase-B_like. This subgroup of the
                     SGNH-family of lipolytic enzymes may have both esterase
                     and phospholipase-A/lysophospholipase activity. It's
                     members may be involved in the conversion of
                     phosphatidylcholine to fatty acids and...; cd01824"
                     /db_xref="CDD:238862"
     Site            order(101,152,212,351,354)
                     /site_type="active"
                     /db_xref="CDD:238862"
     Site            order(101,351,354)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238862"
     Site            order(101,152,212)
                     /site_type="active"
                     /note="oxyanion hole [active]"
                     /db_xref="CDD:238862"
     CDS             1..412
                     /gene="LOC143305582"
                     /coded_by="XM_076689724.1:128..1366"
                     /db_xref="GeneID:143305582"
ORIGIN      
        1 mirmclllql cvliasqrtp ldspenlfvl rnlrkwilqn fgrtdnargf svedrrqiqd
       61 qlppnvpfpc nvtggrsstv pnsvhrlrpg didviaalgd sltagfgifa tdilqiaven
      121 rgvaasiggq gnwrtfltlp nilkefnpnl igyalgdslt sdpasqlnia esgamsrdlp
      181 fmakylinri kkdprinink hwkfitilig sndicneice vsslssflea hrmnlikalr
      241 ifrdnlprtl vsvnvtpnlh tlvdsikgkp slkcyfltqi ecpclfglqy edklseyynv
      301 lhrwqdleee vayypefnrk dftviaspal kklilptaed gfgdmryfsv dcfhvsqrtn
      361 alygnglwnn llepvgnktn yclpeyerfl cptpkkpflm trensfktnk is
//
LOCUS       XP_076545840             390 aa            linear   INV 03-SEP-2025
DEFINITION  phospholipase B1, membrane-associated-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545840
VERSION     XP_076545840.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689725.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="phospholipase B1, membrane-associated-like
                     isoform X2"
                     /calculated_mol_wt=43997
     Region          83..355
                     /region_name="Phospholipase_B_like"
                     /note="Phospholipase-B_like. This subgroup of the
                     SGNH-family of lipolytic enzymes may have both esterase
                     and phospholipase-A/lysophospholipase activity. It's
                     members may be involved in the conversion of
                     phosphatidylcholine to fatty acids and...; cd01824"
                     /db_xref="CDD:238862"
     Site            order(101,152,212,351,354)
                     /site_type="active"
                     /db_xref="CDD:238862"
     Site            order(101,351,354)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238862"
     Site            order(101,152,212)
                     /site_type="active"
                     /note="oxyanion hole [active]"
                     /db_xref="CDD:238862"
     CDS             1..390
                     /gene="LOC143305582"
                     /coded_by="XM_076689725.1:132..1304"
                     /db_xref="GeneID:143305582"
ORIGIN      
        1 mirmclllql cvliasqrtp ldspenlfvl rnlrkwilqn fgrtdnargf svedrrqiqd
       61 qlppnvpfpc nvtggrsstv pnsvhrlrpg didviaalgd sltagfgifa tdilqiaven
      121 rgvaasiggq gnwrtfltlp nilkefnpnl igyalgdslt sdpasqlnia esgamsrdlp
      181 fmakylinri kkdprinink hwkfitilig sndicneice vsslssflea hrmnlikalr
      241 ifrdnlprtl vsvnvtpnlh tlvdsikgkp slkcyfltqi ecpclfglqy edklseyynv
      301 lhrwqdleee vayypefnrk dftviaspal kklilptaed gfgdmryfsv dcfhmvmvcg
      361 tiywsqlvtk pitafqstkd syalhqrshf
//
LOCUS       XP_076545841             388 aa            linear   INV 03-SEP-2025
DEFINITION  phospholipase B1, membrane-associated-like isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545841
VERSION     XP_076545841.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689726.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..388
                     /product="phospholipase B1, membrane-associated-like
                     isoform X3"
                     /calculated_mol_wt=43815
     Region          59..352
                     /region_name="Phospholipase_B_like"
                     /note="Phospholipase-B_like. This subgroup of the
                     SGNH-family of lipolytic enzymes may have both esterase
                     and phospholipase-A/lysophospholipase activity. It's
                     members may be involved in the conversion of
                     phosphatidylcholine to fatty acids and...; cd01824"
                     /db_xref="CDD:238862"
     Site            order(77,128,188,327,330)
                     /site_type="active"
                     /db_xref="CDD:238862"
     Site            order(77,327,330)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238862"
     Site            order(77,128,188)
                     /site_type="active"
                     /note="oxyanion hole [active]"
                     /db_xref="CDD:238862"
     CDS             1..388
                     /gene="LOC143305582"
                     /coded_by="XM_076689726.1:89..1255"
                     /db_xref="GeneID:143305582"
ORIGIN      
        1 mvfllrwcqc necrrlsgrt dnargfsved rrqiqdqlpp nvpfpcnvtg grsstvpnsv
       61 hrlrpgdidv iaalgdslta gfgifatdil qiavenrgva asiggqgnwr tfltlpnilk
      121 efnpnligya lgdsltsdpa sqlniaesga msrdlpfmak ylinrikkdp rininkhwkf
      181 itiligsndi cneicevssl ssfleahrmn likalrifrd nlprtlvsvn vtpnlhtlvd
      241 sikgkpslkc yfltqiecpc lfglqyedkl seyynvlhrw qdleeevayy pefnrkdftv
      301 iaspalkkli lptaedgfgd mryfsvdcfh vsqrtnalyg nglwnnllep vgnktnyclp
      361 eyerflcptp kkpflmtren sfktnkis
//
LOCUS       XP_034185522             312 aa            linear   INV 03-SEP-2025
DEFINITION  thiamine pyrophosphokinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185522
VERSION     XP_034185522.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329631.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185522.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="thiamine pyrophosphokinase 1 isoform X1"
                     /calculated_mol_wt=35217
     Region          59..296
                     /region_name="TPK"
                     /note="Thiamine pyrophosphokinase; cd07995"
                     /db_xref="CDD:153431"
     Site            order(63..65,87..89,121,123..124,146..151,177,179..181,
                     184,188,259,261,273..276)
                     /site_type="active"
                     /db_xref="CDD:153431"
     Site            order(89,97,100,146..148,152,178,180..187,189..190,215,
                     217,239,241,243,245..246,272,274..275,296)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:153431"
     Site            order(121,146..149,259,261,273..276)
                     /site_type="other"
                     /note="thiamine binding site [chemical binding]"
                     /db_xref="CDD:153431"
     CDS             1..312
                     /gene="LOC117606756"
                     /coded_by="XM_034329631.2:75..1013"
                     /db_xref="GeneID:117606756"
ORIGIN      
        1 mhvlkrsisk lsfvanavrq llflrkyqss qamqhdsdsn rtvwnpsqif kcpthykyav
       61 vilnrplywk hdsvlriwek aqinvtvdgg thawlhylee qgidvfngkh neyipnlitg
      121 dmdscsplil erlqsigsrv ietinqdytd ytkaliqlgq yaqkeninln giyvfvdssg
      181 rldhiienln tlhksdklig qyipiipviq iasnsltwil kpgfhtiiip kilvqsnswc
      241 gllpiggpvn citttglkwn lnratlqfgg lisssntydd csevtintds svvwtmgiea
      301 ldeelnckkc ch
//
LOCUS       XP_034185523             478 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606756 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185523
VERSION     XP_034185523.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329632.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185523.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="uncharacterized protein LOC117606756 isoform X2"
                     /calculated_mol_wt=54115
     Region          88..>177
                     /region_name="PRKCSH-like"
                     /note="Glucosidase II beta subunit-like; pfam12999"
                     /db_xref="CDD:372423"
     Region          225..462
                     /region_name="TPK"
                     /note="Thiamine pyrophosphokinase; cd07995"
                     /db_xref="CDD:153431"
     Site            order(229..231,253..255,287,289..290,312..317,343,
                     345..347,350,354,425,427,439..442)
                     /site_type="active"
                     /db_xref="CDD:153431"
     Site            order(255,263,266,312..314,318,344,346..353,355..356,381,
                     383,405,407,409,411..412,438,440..441,462)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:153431"
     Site            order(287,312..315,425,427,439..442)
                     /site_type="other"
                     /note="thiamine binding site [chemical binding]"
                     /db_xref="CDD:153431"
     CDS             1..478
                     /gene="LOC117606756"
                     /coded_by="XM_034329632.2:741..2177"
                     /db_xref="GeneID:117606756"
ORIGIN      
        1 mkteiswkrr llrkklkytl icvfiaaiif vihqlvyfke lnqaivgkli sgsvrtsnyi
       61 vtgkvhskwn dlshskllrd kqgktvslrg trdqdiskyl pnaqgefvcf tskdkidffk
      121 indnycdcpl dgsdepgtna cnngvfhcei stlhfpvkip sykvndgycd ccdgsdewde
      181 vilpysnnvl rkyqssqamq hdsdsnrtvw npsqifkcpt hykyavviln rplywkhdsv
      241 lriwekaqin vtvdggthaw lhyleeqgid vfngkhneyi pnlitgdmds csplilerlq
      301 sigsrvieti nqdytdytka liqlgqyaqk eninlngiyv fvdssgrldh iienlntlhk
      361 sdkligqyip iipviqiasn sltwilkpgf htiiipkilv qsnswcgllp iggpvncitt
      421 tglkwnlnra tlqfggliss sntyddcsev tintdssvvw tmgiealdee lnckkcch
//
LOCUS       XP_034185524             288 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial protein C2orf69 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034185524
VERSION     XP_034185524.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329633.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185524.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="mitochondrial protein C2orf69 homolog"
                     /calculated_mol_wt=33140
     Region          10..259
                     /region_name="C2orf69"
                     /note="pfam10561"
                     /db_xref="CDD:463147"
     CDS             1..288
                     /gene="LOC117606757"
                     /coded_by="XM_034329633.2:13..879"
                     /db_xref="GeneID:117606757"
ORIGIN      
        1 msskiwvwkk vpgivgrcnd viysrpktpp sqdlllyfgg dvqdiqenme qhsdskkyie
       61 wnlqntahil stnfpkkhii virpsriyvt mhalfscfdn fvpgneygip sylpthnglk
      121 hlhelvkscl dhiktcsgse dpslfsieki kltlmgfskg cvvlnqllhe fhyyqrkskp
      181 dveinnftkl iesiwwldgg hagtkdtwit dksilesfak lrinvhvhvt pyqvqdssrp
      241 wnreeenrfs ttlknigvpi krelhftdkp rslllhfnvi kavgnctq
//
LOCUS       XP_034185526             275 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606758 [Osmia lignaria lignaria].
ACCESSION   XP_034185526
VERSION     XP_034185526.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329635.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="uncharacterized protein LOC117606758"
                     /calculated_mol_wt=31806
     CDS             1..275
                     /gene="LOC117606758"
                     /coded_by="XM_034329635.2:844..1671"
                     /db_xref="GeneID:117606758"
ORIGIN      
        1 mgftkifkrm lyanfascst kaigilaplt ipplhiafly yfwqeyardv dkqycscscw
       61 dtvfkgsyes giasykhmyf natsntlkiw vtivigvitf yevvkhltwl afqnrlrlcm
      121 milfstaifs hyytwwvymn ywndefysqw yhqlffsite listflvvhl adnknpvthr
      181 kafgiaaiai lhivaggwdq fvanvvrgeg yahqvvrdlg fmipdilhva iplwaikwkn
      241 iynlpgsakr dpsirrdlay mlgmiciglc icail
//
LOCUS       XP_034185527             263 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606759 [Osmia lignaria lignaria].
ACCESSION   XP_034185527
VERSION     XP_034185527.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329636.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="uncharacterized protein LOC117606759"
                     /calculated_mol_wt=29573
     Region          145..260
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(232,234,244,246..249,252..254)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..263
                     /gene="LOC117606759"
                     /coded_by="XM_034329636.2:107..898"
                     /db_xref="GeneID:117606759"
ORIGIN      
        1 mdstdivlrf liallsinli tcnpccqins yklpsyklmk snarlnqlil srtmvlnvre
       61 cmkfatakka lafnygletd hhdelrhnks ikktqkrcea lqcpevhnlt mlardknyny
      121 ystyptslal gsnytlacip rtgvfvfsnn slnysqaqaa cqkmnaslah viseertngf
      181 akyisqntpt fvglsnhdrk slwknefgep lscfnyrawg ggepshskgc vtlllqskse
      241 lspfwkvvsc nstlpficei ppm
//
LOCUS       XP_076545842             208 aa            linear   INV 03-SEP-2025
DEFINITION  ras-related protein Rab-8A-like [Osmia lignaria lignaria].
ACCESSION   XP_076545842
VERSION     XP_076545842.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689727.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="ras-related protein Rab-8A-like"
                     /calculated_mol_wt=23527
     Region          9..166
                     /region_name="Rab"
                     /note="Ras-related in brain (Rab) family of small
                     guanosine triphosphatases (GTPases); cd00154"
                     /db_xref="CDD:206640"
     Site            9..10
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206640"
     Site            15..22
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206640"
     Site            order(17..23,33,40,66,121..122,124,151..153)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206640"
     Site            23..33
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206640"
     Site            order(33,40..46)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206640"
     Site            40
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206640"
     Site            order(41,43..45,60,62,69..70,73,77,82)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206640"
     Site            order(41..42,44,62..63,70,74..76)
                     /site_type="active"
                     /note="GDI interaction site [active]"
                     /db_xref="CDD:206640"
     Site            41..45
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206640"
     Site            order(42..49,56,58)
                     /site_type="other"
                     /note="GEF interaction site [polypeptide binding]"
                     /db_xref="CDD:206640"
     Site            58..62
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206640"
     Site            63..66
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206640"
     Site            order(66,68..71,73..78)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206640"
     Site            order(69..71,73..74)
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206640"
     Site            77..78
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206640"
     Site            86..91
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206640"
     Site            114..118
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206640"
     Site            121..124
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206640"
     Site            151..153
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206640"
     CDS             1..208
                     /gene="LOC143305583"
                     /coded_by="XM_076689727.1:339..965"
                     /db_xref="GeneID:143305583"
ORIGIN      
        1 maldfaatyk vlvlgdsnvg ktcivhrycd eryydtyist igidfkqkii nldgtpiklq
       61 iwdtagqerf rtlttayyrg amgillmydv tslesfnhls ywlrniqena spdvvkvlaa
      121 nkcdatahra vdaergqkia enfdmpffev sckeniniee afltlarrir eqrgrraslf
      181 easeregads vikaqspsqq gdawrctc
//
LOCUS       XP_034185529             189 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate kinase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185529
VERSION     XP_034185529.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329638.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="adenylate kinase 1"
                     /calculated_mol_wt=20790
     Region          2..187
                     /region_name="aden_kin_iso1"
                     /note="adenylate kinase, isozyme 1 subfamily; TIGR01360"
                     /db_xref="CDD:130427"
     CDS             1..189
                     /gene="Ak1"
                     /coded_by="XM_034329638.2:256..825"
                     /db_xref="GeneID:117606760"
ORIGIN      
        1 mktvwiiggp gcgkgtqcer iiakygfyhi ssgdllreev asgsprgasl qelmskglfv
       61 ptdivldlir ermqkakceg atktgflidg ypreldqgil feknvcpvdl iiffdvanet
      121 lmkrllgraa vskraddnee tikkrieifn ikngeivehy kdkvvrinae gsvdaiflea
      181 skaldglla
//
LOCUS       XP_034185530             189 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate kinase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185530
VERSION     XP_034185530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329639.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="adenylate kinase 1"
                     /calculated_mol_wt=20790
     Region          2..187
                     /region_name="aden_kin_iso1"
                     /note="adenylate kinase, isozyme 1 subfamily; TIGR01360"
                     /db_xref="CDD:130427"
     CDS             1..189
                     /gene="Ak1"
                     /coded_by="XM_034329639.2:239..808"
                     /db_xref="GeneID:117606760"
ORIGIN      
        1 mktvwiiggp gcgkgtqcer iiakygfyhi ssgdllreev asgsprgasl qelmskglfv
       61 ptdivldlir ermqkakceg atktgflidg ypreldqgil feknvcpvdl iiffdvanet
      121 lmkrllgraa vskraddnee tikkrieifn ikngeivehy kdkvvrinae gsvdaiflea
      181 skaldglla
//
LOCUS       XP_034185531             189 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate kinase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185531
VERSION     XP_034185531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329640.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="adenylate kinase 1"
                     /calculated_mol_wt=20790
     Region          2..187
                     /region_name="aden_kin_iso1"
                     /note="adenylate kinase, isozyme 1 subfamily; TIGR01360"
                     /db_xref="CDD:130427"
     CDS             1..189
                     /gene="Ak1"
                     /coded_by="XM_034329640.2:237..806"
                     /db_xref="GeneID:117606760"
ORIGIN      
        1 mktvwiiggp gcgkgtqcer iiakygfyhi ssgdllreev asgsprgasl qelmskglfv
       61 ptdivldlir ermqkakceg atktgflidg ypreldqgil feknvcpvdl iiffdvanet
      121 lmkrllgraa vskraddnee tikkrieifn ikngeivehy kdkvvrinae gsvdaiflea
      181 skaldglla
//
LOCUS       XP_034185532             184 aa            linear   INV 03-SEP-2025
DEFINITION  muscle-specific protein 20 transgelin [Osmia lignaria lignaria].
ACCESSION   XP_034185532
VERSION     XP_034185532.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329641.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..184
                     /product="muscle-specific protein 20 transgelin"
                     /calculated_mol_wt=20399
     Region          14..120
                     /region_name="CH_dMP20-like"
                     /note="calponin homology (CH) domain found in Drosophila
                     melanogaster muscle-specific protein 20 (dMP20) and
                     similar domains; cd21207"
                     /db_xref="CDD:409056"
     Site            order(19,23,74,76..77,80..81,83,93..101,107,109..110,
                     112..113,116..117,120)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409056"
     Region          157..180
                     /region_name="Calponin"
                     /note="Calponin family repeat; pfam00402"
                     /db_xref="CDD:459803"
     CDS             1..184
                     /gene="Mp20"
                     /coded_by="XM_034329641.2:132..686"
                     /db_xref="GeneID:117606761"
ORIGIN      
        1 malerqvrak iagkrnpeqe keaqewiesi lgrkfpqgel fedvikdgqv lcelmnkvsp
       61 gsvpkinstg gqfkmmenin mfqkalkdyg vddvdvfqtv dlwekkdiaq vvttlfalgr
      121 ttykhpewkg pylgpkpade ckrefteeql ragesviglq agsnkgatqs gqsigatrki
      181 llgk
//
LOCUS       XP_034185210             414 aa            linear   INV 03-SEP-2025
DEFINITION  phospholipase B1, membrane-associated [Osmia lignaria lignaria].
ACCESSION   XP_034185210
VERSION     XP_034185210.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329319.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185210.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="phospholipase B1, membrane-associated"
                     /calculated_mol_wt=46177
     Region          78..369
                     /region_name="Phospholipase_B_like"
                     /note="Phospholipase-B_like. This subgroup of the
                     SGNH-family of lipolytic enzymes may have both esterase
                     and phospholipase-A/lysophospholipase activity. It's
                     members may be involved in the conversion of
                     phosphatidylcholine to fatty acids and...; cd01824"
                     /db_xref="CDD:238862"
     Site            order(96,147,207,344,347)
                     /site_type="active"
                     /db_xref="CDD:238862"
     Site            order(96,344,347)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238862"
     Site            order(96,147,207)
                     /site_type="active"
                     /note="oxyanion hole [active]"
                     /db_xref="CDD:238862"
     CDS             1..414
                     /gene="LOC117606621"
                     /coded_by="XM_034329319.2:1..1245"
                     /db_xref="GeneID:117606621"
ORIGIN      
        1 mkikkiclll ilvvlpvvrg simdllrrvf qlgkndvere elkypvmysg irsqpqvpdy
       61 vpfpcnvrag rspivpdnvh rlrpgdidiv gglgdslvag sgaleefaig tfieargvsw
      121 cvggqgdwrr fltvpnilke fnpnlvgyst gtgefistka klniafpvaa tedalhqari
      181 lvhrikrnpr invrqqwkli tiffgandic saqcfspiqf splryalhlq rvldflkial
      241 prtlvnlvpv idvtvsirip rsamcnilhp lycacmhrgn rpdiaaskms hlyqqavetl
      301 vhsgrydnsp dftvvlqpfi kvfnapnadp skappldpsl vtydcfhfsq kghalganll
      361 wnnmlepvgn ktdqgvpeif erilcpseqt pyiftnvnsr hfrltgsqdg tmdr
//
LOCUS       XP_034185500             681 aa            linear   INV 03-SEP-2025
DEFINITION  hexamerin 70a [Osmia lignaria lignaria].
ACCESSION   XP_034185500
VERSION     XP_034185500.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329609.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185500.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..681
                     /product="hexamerin 70a"
                     /calculated_mol_wt=81435
     Region          32..154
                     /region_name="Hemocyanin_N"
                     /note="Hemocyanin, all-alpha domain; pfam03722"
                     /db_xref="CDD:461025"
     Region          160..417
                     /region_name="Hemocyanin_M"
                     /note="Hemocyanin, copper containing domain; pfam00372"
                     /db_xref="CDD:459787"
     Region          437..670
                     /region_name="Hemocyanin_C"
                     /note="Hemocyanin, ig-like domain; pfam03723"
                     /db_xref="CDD:461026"
     CDS             1..681
                     /gene="Hex70a"
                     /coded_by="XM_034329609.2:1..2046"
                     /db_xref="GeneID:117606747"
ORIGIN      
        1 mllltriwvl gllafslvsa eyfttktadk dfllkqkkiy nllyhisqpn vvnvqlyneg
       61 qawniensis lyrnpdavke fmymykngml prgemfspyy pkllkemval frlfyyandf
      121 dtfyntalwa riyvnegqfy falcnavirr pdtkyiqlpp pyemypyayf nsevlekahh
      181 akvfgkldsq ktgpyetyii panysgwyln reynfenkln yftedvglna yyfffrqecp
      241 fwlssnefdf lqyrgeeyly ghkqllnryy lerlsndlpk iedfdwqkpf yagyyptmty
      301 hngmpypqrp dwsnfpvyky kyiqdimdme sritaaidsg filrndskwy niyqedglnm
      361 lgniiegngd synrdfygsi dtlgrkilgy nlepanpyqi fpsaletfss smrdpafyrl
      421 ykriyryyyr ykmrqepytr deivypelki esfvvdkltt yfdqydtsis ngllvedqre
      481 aetslikirq yrlnhkpfnf hitvnsekpm kaafriflgp kydshhklve ipdslkyfye
      541 idnwivdlta gqnkigrnsq dcffvipdqe psevfykkvl kaldgaesft ynervfgfpe
      601 rlllpkgkke gmpfqfflyv spvttevpyt sriwgsykfd krpfgfpldk plydfkydgp
      661 nmmfkniliy hkdefemglt y
//
LOCUS       XP_034185501             679 aa            linear   INV 03-SEP-2025
DEFINITION  hexamerin 70c [Osmia lignaria lignaria].
ACCESSION   XP_034185501
VERSION     XP_034185501.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329610.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185501.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..679
                     /product="hexamerin 70c"
                     /calculated_mol_wt=79772
     Region          32..155
                     /region_name="Hemocyanin_N"
                     /note="Hemocyanin, all-alpha domain; pfam03722"
                     /db_xref="CDD:461025"
     Region          162..423
                     /region_name="Hemocyanin_M"
                     /note="Hemocyanin, copper containing domain; pfam00372"
                     /db_xref="CDD:459787"
     Region          433..669
                     /region_name="Hemocyanin_C"
                     /note="Hemocyanin, ig-like domain; pfam03723"
                     /db_xref="CDD:461026"
     CDS             1..679
                     /gene="Hex70c"
                     /coded_by="XM_034329610.2:90..2129"
                     /db_xref="GeneID:117606748"
ORIGIN      
        1 mlkvalllal ggmlcvaega klsgthtadk nfldkqkkly nillyirqnd lkdtdwyntg
       61 mhydisenmd nyknqtvvkq flwmykrgmf lsrgaisnqy nheqryemql lfdlfytakd
      121 fetfyktacf arifmndgmf tmafttavny rsdcqcvrlp miseiypnlf fesrviqqah
      181 ylkmirgtev qertyiipan ysskymypyv dyeykldyyi edadlnayyy yfrmaspiwl
      241 nnnkrkeicg ecyffihkql marynlerrs ndisriasfd wnkpvyplyf snlmysngva
      301 vpkrhkyasl pfhkynylte vftlenrimd aidsgylidt egrkidiytp gglnmlanvi
      361 egngascnrr yygmydavvr nilgfsfdkp nqnvsipsal emhatnmrdp afymlysrim
      421 sfffrykknq pqyteeelvl pgvefefvsn vdklytyfdk cdtvinnava vdtftngkkf
      481 vmkarrpcln yqpfmynfti nsktkmdatl siflgpaydd vkddmvylkk fyyqfmqmdq
      541 fnvtlvpgpn niqrsssess lttsnmmead yfysklnkai sgsepfvyse edrsfpehlt
      601 lprgrtggmk ykmffflstv ngndptmcln paadkfpqkk slgfpldrpm pdtwnysipn
      661 mffkdvyiye ipkgegmny
//
LOCUS       XP_034185502             622 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 43 [Osmia lignaria lignaria].
ACCESSION   XP_034185502
VERSION     XP_034185502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..622
                     /product="WD repeat-containing protein 43"
                     /calculated_mol_wt=68443
     Region          <8..229
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          8..44
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          50..112
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          117..153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          159..192
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          435..536
                     /region_name="Utp12"
                     /note="Dip2/Utp12 Family; pfam04003"
                     /db_xref="CDD:461125"
     CDS             1..622
                     /gene="LOC117606749"
                     /coded_by="XM_034329611.2:1..1869"
                     /db_xref="GeneID:117606749"
ORIGIN      
        1 mattanyafs pdgeyfahcg ndgklkiwdt gtgrlkqeyv snfhlsspcc vltwlyvnsr
       61 stntsplpwk krrrksvaee psqksmvamg stngkvtlyd tttssvtsql dghsstvtav
      121 twsenvglft agddhhiihw nlqengvkck wksgkgktts ltvsedgkgl lsgervvkww
      181 dlstkqviki ftghanqvtc lcavkiplgs nyvisgaygd gclsvwalde qrndraavas
      241 lalqddaisv svnvsesshv vvlvvtrsgq ahifqyqpng rtkplkpsln iavasdisqk
      301 dsiqqipild akltedqkll laygthinpv fekvtpdfsn kvqclvrsds kknkdrkeei
      361 tkikstvieg nveylapgiv etttkrtkss sgsqlllkdr lenlslgtes sptgkitstg
      421 ssraqlllqg lnskdktils nvfliknesi irntitklpi qaigpllkel tillqgktyt
      481 skiavrwlet llmvhashfl sqadlvdlfg pilnlidakl alltelsklk grvslvtgqi
      541 aqiveeqdkd itenclmyqd sdtsdeddav ededlesesd enweemsnqe dqenqddqee
      601 qnededvkst nsdsdeeksm ss
//
LOCUS       XP_034185505             516 aa            linear   INV 03-SEP-2025
DEFINITION  protein TAPT1 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185505
VERSION     XP_034185505.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329614.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="protein TAPT1 homolog isoform X1"
                     /calculated_mol_wt=58926
     Region          146..454
                     /region_name="DUF747"
                     /note="Eukaryotic membrane protein family; pfam05346"
                     /db_xref="CDD:398815"
     CDS             1..516
                     /gene="LOC117606750"
                     /coded_by="XM_034329614.2:262..1812"
                     /db_xref="GeneID:117606750"
ORIGIN      
        1 mnidenwqnf tekkcirfks tkksdsqsnk iqdvksqfsf ekrrqkgqgm slsqflrtel
       61 trgyqlende erfsarreki ysfmkiprev ekfmtygflq cadsflfvyt flplrfmmal
      121 wtvitrplwy clrkernnlk vgerhlrpae icdllkgivv iscwaatwkv dtsmmyhlvk
      181 sqsviklyif ynmlevgdrl fsafgqdtid allwtatepr sktqtgsqhl gtlphllfav
      241 tyvllhsilv lfqattlnva inssnkallt immsnnfvel kgsvfkkfdk nnlfqlscad
      301 vrerfhlmml llavnlqtmk eyawkaerla vllpdcimll laevlvdwvk hafitrfnel
      361 rstvyrgyti slaydmaqtr qetafsdpsd lvarrmgfip lplgvaigrv lcttitpaak
      421 paniilllla ylilvalril islvvlgkac dliashtkes evvslkhsat stdvknpnla
      481 tamfsnssis lnnvclneav lkeevdneki skevid
//
LOCUS       XP_034185503             516 aa            linear   INV 03-SEP-2025
DEFINITION  protein TAPT1 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185503
VERSION     XP_034185503.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329612.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="protein TAPT1 homolog isoform X1"
                     /calculated_mol_wt=58926
     Region          146..454
                     /region_name="DUF747"
                     /note="Eukaryotic membrane protein family; pfam05346"
                     /db_xref="CDD:398815"
     CDS             1..516
                     /gene="LOC117606750"
                     /coded_by="XM_034329612.2:202..1752"
                     /db_xref="GeneID:117606750"
ORIGIN      
        1 mnidenwqnf tekkcirfks tkksdsqsnk iqdvksqfsf ekrrqkgqgm slsqflrtel
       61 trgyqlende erfsarreki ysfmkiprev ekfmtygflq cadsflfvyt flplrfmmal
      121 wtvitrplwy clrkernnlk vgerhlrpae icdllkgivv iscwaatwkv dtsmmyhlvk
      181 sqsviklyif ynmlevgdrl fsafgqdtid allwtatepr sktqtgsqhl gtlphllfav
      241 tyvllhsilv lfqattlnva inssnkallt immsnnfvel kgsvfkkfdk nnlfqlscad
      301 vrerfhlmml llavnlqtmk eyawkaerla vllpdcimll laevlvdwvk hafitrfnel
      361 rstvyrgyti slaydmaqtr qetafsdpsd lvarrmgfip lplgvaigrv lcttitpaak
      421 paniilllla ylilvalril islvvlgkac dliashtkes evvslkhsat stdvknpnla
      481 tamfsnssis lnnvclneav lkeevdneki skevid
//
LOCUS       XP_034185504             516 aa            linear   INV 03-SEP-2025
DEFINITION  protein TAPT1 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185504
VERSION     XP_034185504.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329613.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="protein TAPT1 homolog isoform X1"
                     /calculated_mol_wt=58926
     Region          146..454
                     /region_name="DUF747"
                     /note="Eukaryotic membrane protein family; pfam05346"
                     /db_xref="CDD:398815"
     CDS             1..516
                     /gene="LOC117606750"
                     /coded_by="XM_034329613.2:196..1746"
                     /db_xref="GeneID:117606750"
ORIGIN      
        1 mnidenwqnf tekkcirfks tkksdsqsnk iqdvksqfsf ekrrqkgqgm slsqflrtel
       61 trgyqlende erfsarreki ysfmkiprev ekfmtygflq cadsflfvyt flplrfmmal
      121 wtvitrplwy clrkernnlk vgerhlrpae icdllkgivv iscwaatwkv dtsmmyhlvk
      181 sqsviklyif ynmlevgdrl fsafgqdtid allwtatepr sktqtgsqhl gtlphllfav
      241 tyvllhsilv lfqattlnva inssnkallt immsnnfvel kgsvfkkfdk nnlfqlscad
      301 vrerfhlmml llavnlqtmk eyawkaerla vllpdcimll laevlvdwvk hafitrfnel
      361 rstvyrgyti slaydmaqtr qetafsdpsd lvarrmgfip lplgvaigrv lcttitpaak
      421 paniilllla ylilvalril islvvlgkac dliashtkes evvslkhsat stdvknpnla
      481 tamfsnssis lnnvclneav lkeevdneki skevid
//
LOCUS       XP_034185506             467 aa            linear   INV 03-SEP-2025
DEFINITION  protein TAPT1 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185506
VERSION     XP_034185506.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329615.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="protein TAPT1 homolog isoform X2"
                     /calculated_mol_wt=53060
     Region          97..405
                     /region_name="DUF747"
                     /note="Eukaryotic membrane protein family; pfam05346"
                     /db_xref="CDD:398815"
     CDS             1..467
                     /gene="LOC117606750"
                     /coded_by="XM_034329615.2:163..1566"
                     /db_xref="GeneID:117606750"
ORIGIN      
        1 mslsqflrte ltrgyqlend eerfsarrek iysfmkipre vekfmtygfl qcadsflfvy
       61 tflplrfmma lwtvitrplw yclrkernnl kvgerhlrpa eicdllkgiv viscwaatwk
      121 vdtsmmyhlv ksqsviklyi fynmlevgdr lfsafgqdti dallwtatep rsktqtgsqh
      181 lgtlphllfa vtyvllhsil vlfqattlnv ainssnkall timmsnnfve lkgsvfkkfd
      241 knnlfqlsca dvrerfhlmm lllavnlqtm keyawkaerl avllpdciml llaevlvdwv
      301 khafitrfne lrstvyrgyt islaydmaqt rqetafsdps dlvarrmgfi plplgvaigr
      361 vlcttitpaa kpaniillll aylilvalri lislvvlgka cdliashtke sevvslkhsa
      421 tstdvknpnl atamfsnssi slnnvclnea vlkeevdnek iskevid
//
LOCUS       XP_034185508             467 aa            linear   INV 03-SEP-2025
DEFINITION  protein TAPT1 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185508
VERSION     XP_034185508.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329617.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="protein TAPT1 homolog isoform X2"
                     /calculated_mol_wt=53060
     Region          97..405
                     /region_name="DUF747"
                     /note="Eukaryotic membrane protein family; pfam05346"
                     /db_xref="CDD:398815"
     CDS             1..467
                     /gene="LOC117606750"
                     /coded_by="XM_034329617.2:26..1429"
                     /db_xref="GeneID:117606750"
ORIGIN      
        1 mslsqflrte ltrgyqlend eerfsarrek iysfmkipre vekfmtygfl qcadsflfvy
       61 tflplrfmma lwtvitrplw yclrkernnl kvgerhlrpa eicdllkgiv viscwaatwk
      121 vdtsmmyhlv ksqsviklyi fynmlevgdr lfsafgqdti dallwtatep rsktqtgsqh
      181 lgtlphllfa vtyvllhsil vlfqattlnv ainssnkall timmsnnfve lkgsvfkkfd
      241 knnlfqlsca dvrerfhlmm lllavnlqtm keyawkaerl avllpdciml llaevlvdwv
      301 khafitrfne lrstvyrgyt islaydmaqt rqetafsdps dlvarrmgfi plplgvaigr
      361 vlcttitpaa kpaniillll aylilvalri lislvvlgka cdliashtke sevvslkhsa
      421 tstdvknpnl atamfsnssi slnnvclnea vlkeevdnek iskevid
//
LOCUS       XP_034185509             338 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase EEF2KMT isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185509
VERSION     XP_034185509.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329618.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185509.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="protein-lysine N-methyltransferase EEF2KMT
                     isoform X1"
                     /calculated_mol_wt=39361
     Region          137..304
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..338
                     /gene="LOC117606751"
                     /coded_by="XM_034329618.2:221..1237"
                     /db_xref="GeneID:117606751"
ORIGIN      
        1 mdynsldins lvkyflccsp intmnahise hnidwvtefe rqqeilektl nselikkypi
       61 kksyqraflk wlmtmiekkd giihesiyit ycnlvsssfd esnhyrhfii dnentdcitm
      121 lestniiseg ttglcswqga ielsnwcles knefcdkvil elgcgvgltg lciikkcfpk
      181 qyiftdchet vlqmlsenvk lnllhneeii ksqtradrlk levkynctnv kimelkweni
      241 dkyinedwii pniiigsdil ydvdsfnalv lglkiflsfn ntyaiiaati rnadtilqfl
      301 qqleihnlsf eeysvskrrv hmqftnlpik ilkifqkr
//
LOCUS       XP_034185510             315 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase EEF2KMT isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185510
VERSION     XP_034185510.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329619.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="protein-lysine N-methyltransferase EEF2KMT
                     isoform X2"
                     /calculated_mol_wt=36725
     Region          114..281
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..315
                     /gene="LOC117606751"
                     /coded_by="XM_034329619.2:212..1159"
                     /db_xref="GeneID:117606751"
ORIGIN      
        1 mnahisehni dwvteferqq eilektlnse likkypikks yqraflkwlm tmiekkdgii
       61 hesiyitycn lvsssfdesn hyrhfiidne ntdcitmles tniisegttg lcswqgaiel
      121 snwcleskne fcdkvilelg cgvgltglci ikkcfpkqyi ftdchetvlq mlsenvklnl
      181 lhneeiiksq tradrlklev kynctnvkim elkwenidky inedwiipni iigsdilydv
      241 dsfnalvlgl kiflsfnnty aiiaatirna dtilqflqql eihnlsfeey svskrrvhmq
      301 ftnlpikilk ifqkr
//
LOCUS       XP_034185511             334 aa            linear   INV 03-SEP-2025
DEFINITION  geranylgeranyl transferase type-2 subunit beta [Osmia lignaria
            lignaria].
ACCESSION   XP_034185511
VERSION     XP_034185511.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329620.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..334
                     /product="geranylgeranyl transferase type-2 subunit beta"
                     /calculated_mol_wt=37452
     Region          17..300
                     /region_name="GGTase-II"
                     /note="Geranylgeranyltransferase type II (GGTase-II)_like
                     proteins containing the protein prenyltransferase (PTase)
                     domain, beta subunit (alpha 6 - alpha 6 barrel fold).
                     GGTase-IIs are a subgroup of the protein prenyltransferase
                     family of lipid-modifying...; cd02894"
                     /db_xref="CDD:239224"
     Site            order(40,43..44,47,51,93,95..96,98..99,143,146..147,189,
                     191,195,237,239..240,243,279,288..289)
                     /site_type="active"
                     /note="active site cavity [active]"
                     /db_xref="CDD:239224"
     Site            order(43..44,47,95..96,143,237,279)
                     /site_type="active"
                     /note="peptide binding pocket [active]"
                     /db_xref="CDD:239224"
     Site            order(47,50,143,146,189,191,195,231,234,240,243,288)
                     /site_type="lipid-binding"
                     /note="lipid binding pocket [chemical binding]"
                     /db_xref="CDD:239224"
     Site            order(138,176,186..187,192,216,219,221..222,231..233)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:239224"
     Site            order(237,239,289)
                     /site_type="other"
                     /note="Zn2+ binding site [ion binding]"
                     /db_xref="CDD:239224"
     CDS             1..334
                     /gene="betaggt-II"
                     /coded_by="XM_034329620.2:253..1257"
                     /db_xref="GeneID:117606752"
ORIGIN      
        1 mpmskhdiev ptsipdllle khtnfllsyg tdkdeyiycm tehmrmsgmy wgltaldlmg
       61 kleqtnrnev lefiaqcqte sggiaaslqh dphilytlsa vqilciydal dtidiervik
      121 yvkerqqpdg sftgdiwgev dmrfsfcava tlsllhrlda idvdkavefv mkcmnfdggf
      181 gskpgaesha gmiycsigll sitgnlhlvd adqlgwwlce rqlssgglng rpeklpdvcy
      241 swwvlsalti lgrlhwvnke qlvkfvlacq daesggfsdr pgdvadpfht lfgltalsll
      301 ntdyplkkin ptycmpeyii qrlqlkpsrl dqin
//
LOCUS       XP_034185514             156 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606753 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185514
VERSION     XP_034185514.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329623.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..156
                     /product="uncharacterized protein LOC117606753 isoform X1"
                     /calculated_mol_wt=18200
     CDS             1..156
                     /gene="LOC117606753"
                     /coded_by="XM_034329623.2:589..1059"
                     /db_xref="GeneID:117606753"
ORIGIN      
        1 mnlpistsdf fvtasdcgel ltrvkldieq lesamqhhid dylkevidtn vktdadnkkq
       61 nidfdkhmel wmndlkqqli nlqffgraat lrifvyytny ldilrapikk shhqpyklpv
      121 yniefpkneq tkptlllkdl sklkrkkclp kistcn
//
LOCUS       XP_034185512             156 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606753 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185512
VERSION     XP_034185512.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329621.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..156
                     /product="uncharacterized protein LOC117606753 isoform X1"
                     /calculated_mol_wt=18200
     CDS             1..156
                     /gene="LOC117606753"
                     /coded_by="XM_034329621.2:420..890"
                     /db_xref="GeneID:117606753"
ORIGIN      
        1 mnlpistsdf fvtasdcgel ltrvkldieq lesamqhhid dylkevidtn vktdadnkkq
       61 nidfdkhmel wmndlkqqli nlqffgraat lrifvyytny ldilrapikk shhqpyklpv
      121 yniefpkneq tkptlllkdl sklkrkkclp kistcn
//
LOCUS       XP_034185513             156 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606753 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185513
VERSION     XP_034185513.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329622.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..156
                     /product="uncharacterized protein LOC117606753 isoform X1"
                     /calculated_mol_wt=18200
     CDS             1..156
                     /gene="LOC117606753"
                     /coded_by="XM_034329622.2:528..998"
                     /db_xref="GeneID:117606753"
ORIGIN      
        1 mnlpistsdf fvtasdcgel ltrvkldieq lesamqhhid dylkevidtn vktdadnkkq
       61 nidfdkhmel wmndlkqqli nlqffgraat lrifvyytny ldilrapikk shhqpyklpv
      121 yniefpkneq tkptlllkdl sklkrkkclp kistcn
//
LOCUS       XP_076545843              89 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606753 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545843
VERSION     XP_076545843.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689728.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="uncharacterized protein LOC117606753 isoform X2"
                     /calculated_mol_wt=10205
     CDS             1..89
                     /gene="LOC117606753"
                     /coded_by="XM_076689728.1:589..858"
                     /db_xref="GeneID:117606753"
ORIGIN      
        1 mnlpistsdf fvtasdcgel ltrvkldieq lesamqhhid dylkevidtn vktdadnkkq
       61 nidfdkhmel wmndlkqqli nlqffgcda
//
LOCUS       XP_034185515              89 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606753 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185515
VERSION     XP_034185515.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329624.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="uncharacterized protein LOC117606753 isoform X3"
                     /calculated_mol_wt=10672
     CDS             1..89
                     /gene="LOC117606753"
                     /coded_by="XM_034329624.2:75..344"
                     /db_xref="GeneID:117606753"
ORIGIN      
        1 mlnfqmvfqw gkngnllfip fqvlrsvsrf rkhqldlfrn mkriylygkf lydrrrfhcc
       61 ngqclellfq gkisyslsvp lindenisf
//
LOCUS       XP_034185518              80 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606753 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185518
VERSION     XP_034185518.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329627.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..80
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..80
                     /product="uncharacterized protein LOC117606753 isoform X4"
                     /calculated_mol_wt=9635
     CDS             1..80
                     /gene="LOC117606753"
                     /coded_by="XM_034329627.2:75..317"
                     /db_xref="GeneID:117606753"
ORIGIN      
        1 mlnfqmvfqw gkngnllfip fqvlrsvsrf rkhqldlfrn mkriylygkf lydrrrfhcc
       61 ngekqmivtl iamsenksvi
//
LOCUS       XP_076545844              66 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606753 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545844
VERSION     XP_076545844.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689729.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..66
                     /product="uncharacterized protein LOC117606753 isoform X5"
                     /calculated_mol_wt=8093
     CDS             1..66
                     /gene="LOC117606753"
                     /coded_by="XM_076689729.1:75..275"
                     /db_xref="GeneID:117606753"
ORIGIN      
        1 mlnfqmvfqw gkngnllfip fqvlrsvsrf rkhqldlfrn mkriylygkf lydrrrfhcc
       61 nekgcn
//
LOCUS       XP_034194833             106 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611052 [Osmia lignaria lignaria].
ACCESSION   XP_034194833
VERSION     XP_034194833.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338942.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="uncharacterized protein LOC117611052"
                     /calculated_mol_wt=11684
     CDS             1..106
                     /gene="LOC117611052"
                     /coded_by="XM_034338942.2:95..415"
                     /db_xref="GeneID:117611052"
ORIGIN      
        1 mkvnqgkrsr rptrrggvkq raralikrqi qlvqkeletr fppsgpsshp gepcyspvse
       61 asticlgprs pqlidlteep elesvnvaet neipaeilai ifnlqe
//
LOCUS       XP_076545845            1132 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609781 [Osmia lignaria lignaria].
ACCESSION   XP_076545845
VERSION     XP_076545845.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689730.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1132
                     /product="uncharacterized protein LOC117609781"
                     /calculated_mol_wt=130073
     Region          672..862
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(728,762,772,774)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..1132
                     /gene="LOC117609781"
                     /coded_by="XM_076689730.1:10596..13994"
                     /db_xref="GeneID:117609781"
ORIGIN      
        1 mkidaavvfe ekynvplksf gktvkddsqt dmsdknpsfl pnnvkdvgri lsenalcenn
       61 dvgrilsena lrkndetvvv qcectserlt vqnvldnsdd nfvvgvghfd vgrisdnvpe
      121 grrvvdvhff wneihrtfdn hargiechfr dwkmigsrqh gcltqfffkc qmcnyedtvw
      181 sepmhsditn vneamttatv tvgigsaqle elcaalnmpc maettyrryr eklvdefmks
      241 amhtmqaage eerrlayenn evvdgipyit viadgswmkr sygtnynsls gvgailgfrt
      301 gkvlfigirn kycticdiae rkakspkhhk cyknydryas sssmesdaia egfncsiqmh
      361 gliyrtvvad gdsnvyhtiv nnrpyreqkv tvrkvectnh llrnlckklr yvaektqtkg
      421 qrargyvayr niikknilni rravvlavna rkqekgpqhi karelqkdil sipshifgeh
      481 kecnsrnhtc mpdvnraekn yvpslklfgl ysevekairf lscysdsllm nvtnnpaetf
      541 nsiickeigg krinfgargs ynarvagavt qyntqqvlte mhknmcktvp plvkrleerr
      601 qkkvaktkqf rmtygktrkl kpvqgtdkyy gpnsqrpdlp sdmllqlrke hfqkladnak
      661 nrtiietntr eqndsdlwrt lkeemltasn fgavcrmrqt tscaamvkni lyppfvdtaa
      721 mkygrdkehi arkdvanamk trirtcglfi drdipylgas pdglidddgl lelkcpqsae
      781 nltatdaiet irhlrnifdk rdiqemnrkh qyfyqvqgql hitqrkycif aiwtpqsihm
      841 ihvnrddafw tnhmepfltr fyeecmlpei ldsrhnrhmp irnpeyilra kedasiqkss
      901 rkitkkrtye nmiqnsveas nmtveavnse cvivshsdqe ekfidddvgy yiletavssv
      961 tdvqknvlpv qsklndelld rflrvvreta pfetqsvlyl qftkfikpcc sdksvqiigg
     1021 ncsdhwrsiy fdgrklhvyd slpgsaydkl veeekkyial rfphirkndi lfervqaqpd
     1081 cyscgvyaaa yittiilggn pcsenyshdi qrlrchfvni iennrlspfp te
//
LOCUS       XP_076545846            1132 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609781 [Osmia lignaria lignaria].
ACCESSION   XP_076545846
VERSION     XP_076545846.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689731.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1132
                     /product="uncharacterized protein LOC117609781"
                     /calculated_mol_wt=130073
     Region          672..862
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(728,762,772,774)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..1132
                     /gene="LOC117609781"
                     /coded_by="XM_076689731.1:396..3794"
                     /db_xref="GeneID:117609781"
ORIGIN      
        1 mkidaavvfe ekynvplksf gktvkddsqt dmsdknpsfl pnnvkdvgri lsenalcenn
       61 dvgrilsena lrkndetvvv qcectserlt vqnvldnsdd nfvvgvghfd vgrisdnvpe
      121 grrvvdvhff wneihrtfdn hargiechfr dwkmigsrqh gcltqfffkc qmcnyedtvw
      181 sepmhsditn vneamttatv tvgigsaqle elcaalnmpc maettyrryr eklvdefmks
      241 amhtmqaage eerrlayenn evvdgipyit viadgswmkr sygtnynsls gvgailgfrt
      301 gkvlfigirn kycticdiae rkakspkhhk cyknydryas sssmesdaia egfncsiqmh
      361 gliyrtvvad gdsnvyhtiv nnrpyreqkv tvrkvectnh llrnlckklr yvaektqtkg
      421 qrargyvayr niikknilni rravvlavna rkqekgpqhi karelqkdil sipshifgeh
      481 kecnsrnhtc mpdvnraekn yvpslklfgl ysevekairf lscysdsllm nvtnnpaetf
      541 nsiickeigg krinfgargs ynarvagavt qyntqqvlte mhknmcktvp plvkrleerr
      601 qkkvaktkqf rmtygktrkl kpvqgtdkyy gpnsqrpdlp sdmllqlrke hfqkladnak
      661 nrtiietntr eqndsdlwrt lkeemltasn fgavcrmrqt tscaamvkni lyppfvdtaa
      721 mkygrdkehi arkdvanamk trirtcglfi drdipylgas pdglidddgl lelkcpqsae
      781 nltatdaiet irhlrnifdk rdiqemnrkh qyfyqvqgql hitqrkycif aiwtpqsihm
      841 ihvnrddafw tnhmepfltr fyeecmlpei ldsrhnrhmp irnpeyilra kedasiqkss
      901 rkitkkrtye nmiqnsveas nmtveavnse cvivshsdqe ekfidddvgy yiletavssv
      961 tdvqknvlpv qsklndelld rflrvvreta pfetqsvlyl qftkfikpcc sdksvqiigg
     1021 ncsdhwrsiy fdgrklhvyd slpgsaydkl veeekkyial rfphirkndi lfervqaqpd
     1081 cyscgvyaaa yittiilggn pcsenyshdi qrlrchfvni iennrlspfp te
//
LOCUS       XP_076545847             679 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305586 [Osmia lignaria lignaria].
ACCESSION   XP_076545847
VERSION     XP_076545847.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689732.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..679
                     /product="uncharacterized protein LOC143305586"
                     /calculated_mol_wt=78693
     CDS             1..679
                     /gene="LOC143305586"
                     /coded_by="XM_076689732.1:12565..14604"
                     /db_xref="GeneID:143305586"
ORIGIN      
        1 miyivvnlis rrrlvdlmfd redlqfsdkl eyiesyitty dnyssenvqd irkrfsyiks
       61 elkrrwseaq ntkdrfykkn nnwlngtfel pvvtvrsgrp sksfeessdr tkrrktavlr
      121 sttntdelvf saqmklqssg krsastilke itnsperakk yktaysifqh rsnetlqlss
      181 lkalsmfvea glskhqylii rksnhlfyps ynkllkekln cypqkesyqv tstcaevnlq
      241 dllnhtatrl ltylehvlin ldedecksls liskwgcdgs qqshfkqafd nefesdenif
      301 qssfvplqln calsgkklvw qnptpsspry crpirirfvk etfdvitsei dyirnainsl
      361 qptkltlfgr dfsiqhkmil tmvdgkicna itqtlstrrc yicgatstef ndltklqvkd
      421 inpdslefgl silharirlf esllhlsykv tvkkwiirkt dvqqkkiied rklniqkefk
      481 sklgllvdip kpgfgntnng ntsrkffanp dlcaeitgld rdliyrfavi lelissghki
      541 ntkkfdtytl dtaklyinly swypmtptmh killhgsiii ehallpigql seeaaearnk
      601 hfrlyrqnys rkfsrescnl dvinrlllts dplltgirkm prkrtkqflk etiemflpae
      661 tprtaedsss deqlsdgdl
//
LOCUS       XP_076545848             302 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117609776 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545848
VERSION     XP_076545848.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689733.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..302
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117609776"
                     /calculated_mol_wt=34174
     CDS             1..302
                     /gene="LOC117609776"
                     /coded_by="XM_076689733.1:147..1055"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:117609776"
ORIGIN      
        1 mevdaiiemf srsdekfgvk ygnyigdgds ktfkaimdvn pygdnfqvtk necighvekr
       61 mgsrlrnikk tknlggkgkl tdalirklta yyglairrni dsvenmkkai masyyhlcst
      121 kenprheycp vgndswckwq kaliatganl dliehpaplh pdvqkhilpi yadlseenll
      181 erclgghtqn nnesfnstvw rfapkhlhsg ikiieiaayl aaglfnegya svlrtmsaln
      241 ivigkqakty adkideqrii rqerrtsltt kearktrreq rmeknqlyee tegilygagi
      301 ad
//
LOCUS       XP_034185535            1324 aa            linear   INV 03-SEP-2025
DEFINITION  SREBP cleavage activating protein [Osmia lignaria lignaria].
ACCESSION   XP_034185535
VERSION     XP_034185535.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329644.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1324
                     /product="SREBP cleavage activating protein"
                     /calculated_mol_wt=149380
     Region          <9..>432
                     /region_name="2A060601"
                     /note="Niemann-Pick C type protein family; TIGR00917"
                     /db_xref="CDD:273337"
     Region          292..432
                     /region_name="Sterol-sensing"
                     /note="Sterol-sensing domain of SREBP cleavage-activation;
                     pfam12349"
                     /db_xref="CDD:463544"
     Region          <762..>803
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          944..1231
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(953..954,979,983,988,1000..1001,1016,1020,
                     1026..1027,1040,1056,1061,1080,1109,1120..1121,1137,1141,
                     1147..1148,1160..1161,1184,1190..1191,1203..1204,1220,
                     1224,1230..1231)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          998..1040
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1045..1074
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1082..1116
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <1119..1289
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1166..1203
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1208..1242
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1248..1283
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..1324
                     /gene="SCAP"
                     /coded_by="XM_034329644.2:588..4562"
                     /db_xref="GeneID:117606765"
ORIGIN      
        1 mfrslpdrva glyyahglfc sshpvavisl aisivmlccy plvnlpmpgn apravinhti
       61 vpenstenhv fyvqqvvlrv gvipwteelt lmdafrgply eifnlleiiq nyqhpetlkt
      121 lgqvclhvea vkrngkksdv lpeynclvls panlwqrsie lyaqdtnlin tiysyqnlqk
      181 gkislaeimf gmnlketgtk rypsrlrqri lqyavtiylk dydpefirgl khrlktyyhl
      241 hqvgdndtyd rvdetlhily pgefnysdff plmmtffalf fyvyfsvrki elikskigia
      301 fsatvtviss lsmtvgvcff fgltlslsgk evfpylviiv glenvliltk svvstpthld
      361 vkirvaqals kegwsitknl ltevtiltig lftfvpaiqe fcifaivglv ndfflqmvff
      421 stilaidikr telssetskf hlpnipttrk qqftttitnr kpnifrsksh prlnglatgp
      481 tnviapntqn thtlgkipkr lrlvhfwart rifqrafmvw mvvwismiiy nsgivehvih
      541 lsetlkpesd idgytvdrsh alnnyvelnt mkplsipssa vapdhlnkqn dltnniteel
      601 nklrpvdfpp wnrlslyhws silsmynisa aggritilpa vkishavspq lakqisnpnd
      661 vqhfqwqsla taaldpldfs dmelptrseg rgfnadapfi psspmeifla avlclisviv
      721 vaytmvvlyr cicsrnyaew raswyqpeka hdsatqlvle alplvleght qeveciatds
      781 ntiastclag hirvwdatsg eqlvhidrkq ffsspkknln hvtsdmdelm sdyesgspps
      841 rgemegnnsf glyssattvp yrksspglyn ihrgqntnrn hsmgntfdyd yqinefgver
      901 rknvrrslds yydlpdlkst invkfstmkr sslqqnyehg fdfgdhykql feehnrside
      961 lqksesfeql yipngkvnss glvdsissls tdrtvqimhs vspiwcmdyq enlivvgcan
     1021 gslefwegtt grfkclfddg sglgisavkf vgsrvvaakl ngsidflqle sysegqqidw
     1081 gftsyrrthv rtgsagspmd innimqsedl rcmkigshka hqqaitvlds vggrvltgsq
     1141 dhtlkvyrle dqlplytlhg hcgpisclfi drmspmtsgs gsqdgllcvw dlqtgtcmys
     1201 iqahdgavaa itcsvsyvis igtderlcvw erfqghllha lpahrsaysl qlvmlthhll
     1261 itsnqgsliv wdvrtgepvr evrlghkdsc ifvkqmlvlr dsvvcdfgrq lrivrfplvs
     1321 dkld
//
LOCUS       XP_034185543            1102 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glycine receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185543
VERSION     XP_034185543.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329652.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185543.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1102
                     /product="metabotropic glycine receptor isoform X1"
                     /calculated_mol_wt=124598
     Region          324..505
                     /region_name="PDC1_MCP_like"
                     /note="first PDC (PhoQ/DcuS/CitA) domain of
                     methyl-accepting chemotaxis proteins and similar domains;
                     cd12913"
                     /db_xref="CDD:350338"
     Region          622..871
                     /region_name="7tmC_GPR158-like"
                     /note="orphan GPR158 and similar proteins, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15293"
                     /db_xref="CDD:320420"
     Region          622..647
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320420"
     Site            order(625,628..629,632..633,635..636,670,674,677..678,686,
                     690)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:320420"
     Region          659..680
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320420"
     Site            order(679,691..692,695..696,699,773,778,781..782,785,819,
                     822..823,826,830,842..843,846,849,853)
                     /site_type="other"
                     /note="putative allosteric modulator binding site
                     [chemical binding]"
                     /db_xref="CDD:320420"
     Region          689..713
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320420"
     Region          732..752
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320420"
     Region          774..800
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320420"
     Region          808..831
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320420"
     Region          840..865
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320420"
     CDS             1..1102
                     /gene="LOC117606766"
                     /coded_by="XM_034329652.2:481..3789"
                     /db_xref="GeneID:117606766"
ORIGIN      
        1 msvpchwyvl vlvtwspvla dfadqeaqie rpkellrhqq nhlpsfierr lvdrpemqea
       61 telsvhlddr lpdenqsenq psrrgilets ierpitqirh srsrshfarq akldepdseg
      121 tnkddplekg gvvrsdrseg lvilakdlrs sgtiderstv dldklltrls eaakegksrn
      181 tpvrhkgdaf kklkkviarn rsrvlgnkta gnmtdvyghi vhgapdrnel ddveiaiied
      241 ydlygdndsg rnnseredye dyggkgdqdr pskepvhvdi vtrflriien qhllgencta
      301 gtdlnlgegi vdqyaqerfk leanlavnra nmltrlwkya pevmlsseyl lhasilsmve
      361 fdedifaagn cydklqyrdh wlycpfahrl qdqdgilvkd laieykyltn ssewfyiark
      421 naerviannd qfsrgfhtyt lnesthtere edeiltvrye dgrwskpyyd cgggniwmlt
      481 ytvpffgyvn dtyffkgtsg ididlrrldi dqcplppgst qlnifaasdk ckkrttecia
      541 ipglgfrrgs yrcvckrgfy ypdtkstsry yngtvieeey eklmmseesq ydvdgvfecl
      601 pcaegcesce ddspcvvsln wlmrtailil eccviaclpa valftwkygn vkvvraaspv
      661 llrvivlgaf fiycttivmy prpnvitctv rvwlreigfs ltygalmlkt wrisvifrvk
      721 sakavkitdg sllkrlgviv mifsvflsir tlvappvviv artaddlkay lcrtdwwdhs
      781 fttlevmfli wgirlcivvr ktpsefnesr fismaiynef llsvflnvsm lflqspanpd
      841 llyviffcht qltvtlllcl ifgskayvvf rsggkddskh fgatgkflgk ssrpagtsnq
      901 tnsislqqgn ftdesdgate efrrfinqle vlkeknvllg nhhlvnklaa mqeaanraet
      961 qvstiqaiss smrlndlngs taiaetevge pyksdekggd tkekrcqaci ekngikskdq
     1021 gsskdaevlv sieskkarrt ndvavstsgk atttedigte tkldekesdk kdksksksgh
     1081 arthaivinl ddksrfseev tv
//
LOCUS       XP_034185544            1079 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glycine receptor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185544
VERSION     XP_034185544.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329653.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185544.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1079
                     /product="metabotropic glycine receptor isoform X2"
                     /calculated_mol_wt=122108
     Region          324..505
                     /region_name="PDC1_MCP_like"
                     /note="first PDC (PhoQ/DcuS/CitA) domain of
                     methyl-accepting chemotaxis proteins and similar domains;
                     cd12913"
                     /db_xref="CDD:350338"
     Region          622..848
                     /region_name="7tmC_GPR158-like"
                     /note="orphan GPR158 and similar proteins, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15293"
                     /db_xref="CDD:320420"
     Region          622..647
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320420"
     Site            order(656,668..669,672..673,676,750,755,758..759,762,796,
                     799..800,803,807,819..820,823,826,830)
                     /site_type="other"
                     /note="putative allosteric modulator binding site
                     [chemical binding]"
                     /db_xref="CDD:320420"
     Region          666..690
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320420"
     Region          709..729
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320420"
     Region          751..777
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320420"
     Region          785..808
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320420"
     Region          817..842
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320420"
     CDS             1..1079
                     /gene="LOC117606766"
                     /coded_by="XM_034329653.2:483..3722"
                     /db_xref="GeneID:117606766"
ORIGIN      
        1 msvpchwyvl vlvtwspvla dfadqeaqie rpkellrhqq nhlpsfierr lvdrpemqea
       61 telsvhlddr lpdenqsenq psrrgilets ierpitqirh srsrshfarq akldepdseg
      121 tnkddplekg gvvrsdrseg lvilakdlrs sgtiderstv dldklltrls eaakegksrn
      181 tpvrhkgdaf kklkkviarn rsrvlgnkta gnmtdvyghi vhgapdrnel ddveiaiied
      241 ydlygdndsg rnnseredye dyggkgdqdr pskepvhvdi vtrflriien qhllgencta
      301 gtdlnlgegi vdqyaqerfk leanlavnra nmltrlwkya pevmlsseyl lhasilsmve
      361 fdedifaagn cydklqyrdh wlycpfahrl qdqdgilvkd laieykyltn ssewfyiark
      421 naerviannd qfsrgfhtyt lnesthtere edeiltvrye dgrwskpyyd cgggniwmlt
      481 ytvpffgyvn dtyffkgtsg ididlrrldi dqcplppgst qlnifaasdk ckkrttecia
      541 ipglgfrrgs yrcvckrgfy ypdtkstsry yngtvieeey eklmmseesq ydvdgvfecl
      601 pcaegcesce ddspcvvsln wlmrtailil eccviaclpa valftwkygn vktivmyprp
      661 nvitctvrvw lreigfslty galmlktwri svifrvksak avkitdgsll krlgvivmif
      721 svflsirtlv appvvivart addlkaylcr tdwwdhsftt levmfliwgi rlcivvrktp
      781 sefnesrfis maiyneflls vflnvsmlfl qspanpdlly viffchtqlt vtlllclifg
      841 skayvvfrsg gkddskhfga tgkflgkssr pagtsnqtns islqqgnftd esdgateefr
      901 rfinqlevlk eknvllgnhh lvnklaamqe aanraetqvs tiqaisssmr lndlngstai
      961 aetevgepyk sdekggdtke krcqaciekn gikskdqgss kdaevlvsie skkarrtndv
     1021 avstsgkatt tedigtetkl dekesdkkdk sksksghart haivinlddk srfseevtv
//
LOCUS       XP_034185545             517 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1 [Osmia lignaria lignaria].
ACCESSION   XP_034185545
VERSION     XP_034185545.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329654.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185545.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..517
                     /product="facilitated trehalose transporter Tret1"
                     /calculated_mol_wt=56724
     Region          41..475
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(52..53,56..57,60,95,145..146,148..150,153,173,
                     176..177,180,286,289..290,293..295,298,325,329,391..392,
                     396,400,416,419..420,423..424,427)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..517
                     /gene="LOC117606767"
                     /coded_by="XM_034329654.2:1355..2908"
                     /db_xref="GeneID:117606767"
ORIGIN      
        1 mplsvnnnss ndektspveq ngnsesfsis klrqalpqcc avsaknllmi tfgstlgfst
       61 ilipelqken seipvtteel twisslnlfl vpigcfvsgp isqylgrkkt mmlttipfia
      121 awiiyyyats agmlfialam tgltggllea pvmtyvaevt qphlrgmlsa tssmsiilgi
      181 ftqmlggklg nwrtvslvnl ayplicfval clvpespywl aakgrtreae qalcwlrgwv
      241 spaqvqsefr iicqdvhkpa eskekiwksf skrtfympfl lvtcaffiga fggtitlqtf
      301 avmifvklna pieeytaavf lglaelvgtl icvlaihfvg krvlnfvsig gtglsfclaa
      361 iygylndsqv idvekytwmp ttlmigaafl shagirllpw vlagevfpvk vrssatgaag
      421 smgyiftsta nkvflymvng mslagtflfy alinfvggcl lyfilpeteg rtlkeieehf
      481 agvqnlknrp nkeevqfkek waatnpaiiy ddneskl
//
LOCUS       XP_034185546             274 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185546
VERSION     XP_034185546.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329655.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..274
                     /product="RAS oncogene family member Rab3 isoform X1"
                     /calculated_mol_wt=31146
     Region          72..236
                     /region_name="Rab3"
                     /note="Rab GTPase family 3 contains Rab3A, Rab3B, Rab3C
                     and Rab3D; cd01865"
                     /db_xref="CDD:206657"
     Site            order(72,105,107..109,124,126,133..134,137,141,143..146,
                     175..177,233,236)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206657"
     Site            72..74
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206657"
     Site            79..86
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206657"
     Site            order(81..87,97..98,101,103..104,130,185..186,188..189,
                     215..217)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206657"
     Site            order(87..98,101..103)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206657"
     Site            order(97,102..110)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206657"
     Site            order(102,106..113,120,122)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206657"
     Site            104
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206657"
     Site            order(105..106,108,126..127,134,136,138..140)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206657"
     Site            105..109
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206657"
     Site            122..126
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206657"
     Site            127..130
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206657"
     Site            order(130,132..142)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206657"
     Site            133..138
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206657"
     Site            141..145
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206657"
     Site            150..155
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206657"
     Site            173..182
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206657"
     Site            185..188
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206657"
     Site            215..217
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206657"
     Site            231..236
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206657"
     CDS             1..274
                     /gene="Rab3"
                     /coded_by="XM_034329655.2:113..937"
                     /db_xref="GeneID:117606768"
ORIGIN      
        1 mrrfylktee kaqrdeaqnq rgpavkdsvl emrtrmsrlm siveitpqsr lpmagqdpkm
       61 qkeaadqnfd ymfklliign ssvgktsflf ryaddsftsa fvstvgidfk vktvfrhdkr
      121 vklqiwdtag qeryrtitta yyrgamgfil myditneesf nsvqdwvtqi ktyscdnaqv
      181 ilvgnkcdme dervisferg kqladqlgvq ffetsakeni niktvfeqlv diicdkmses
      241 ldndptllag gmgqakgqrl tdqpanqssd ncnc
//
LOCUS       XP_034185547             233 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185547
VERSION     XP_034185547.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329656.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="RAS oncogene family member Rab3 isoform X2"
                     /calculated_mol_wt=26418
     Region          31..195
                     /region_name="Rab3"
                     /note="Rab GTPase family 3 contains Rab3A, Rab3B, Rab3C
                     and Rab3D; cd01865"
                     /db_xref="CDD:206657"
     Site            order(31,64,66..68,83,85,92..93,96,100,102..105,134..136,
                     192,195)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206657"
     Site            31..33
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206657"
     Site            38..45
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206657"
     Site            order(40..46,56..57,60,62..63,89,144..145,147..148,
                     174..176)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206657"
     Site            order(46..57,60..62)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206657"
     Site            order(56,61..69)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206657"
     Site            order(61,65..72,79,81)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206657"
     Site            63
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206657"
     Site            order(64..65,67,85..86,93,95,97..99)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206657"
     Site            64..68
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206657"
     Site            81..85
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206657"
     Site            86..89
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206657"
     Site            order(89,91..101)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206657"
     Site            92..97
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206657"
     Site            100..104
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206657"
     Site            109..114
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206657"
     Site            132..141
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206657"
     Site            144..147
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206657"
     Site            174..176
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206657"
     Site            190..195
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206657"
     CDS             1..233
                     /gene="Rab3"
                     /coded_by="XM_034329656.2:210..911"
                     /db_xref="GeneID:117606768"
ORIGIN      
        1 megnthrfws wmagqdpkmq keaadqnfdy mfklliigns svgktsflfr yaddsftsaf
       61 vstvgidfkv ktvfrhdkrv klqiwdtagq eryrtittay yrgamgfilm yditneesfn
      121 svqdwvtqik tyscdnaqvi lvgnkcdmed ervisfergk qladqlgvqf fetsakenin
      181 iktvfeqlvd iicdkmsesl dndptllagg mgqakgqrlt dqpanqssdn cnc
//
LOCUS       XP_034185548             222 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab3 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185548
VERSION     XP_034185548.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329657.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="RAS oncogene family member Rab3 isoform X3"
                     /calculated_mol_wt=24985
     Region          20..184
                     /region_name="Rab3"
                     /note="Rab GTPase family 3 contains Rab3A, Rab3B, Rab3C
                     and Rab3D; cd01865"
                     /db_xref="CDD:206657"
     Site            order(20,53,55..57,72,74,81..82,85,89,91..94,123..125,181,
                     184)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206657"
     Site            20..22
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206657"
     Site            27..34
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206657"
     Site            order(29..35,45..46,49,51..52,78,133..134,136..137,
                     163..165)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206657"
     Site            order(35..46,49..51)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206657"
     Site            order(45,50..58)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206657"
     Site            order(50,54..61,68,70)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206657"
     Site            52
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206657"
     Site            order(53..54,56,74..75,82,84,86..88)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206657"
     Site            53..57
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206657"
     Site            70..74
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206657"
     Site            75..78
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206657"
     Site            order(78,80..90)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206657"
     Site            81..86
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206657"
     Site            89..93
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206657"
     Site            98..103
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206657"
     Site            121..130
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206657"
     Site            133..136
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206657"
     Site            163..165
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206657"
     Site            179..184
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206657"
     CDS             1..222
                     /gene="Rab3"
                     /coded_by="XM_034329657.2:146..814"
                     /db_xref="GeneID:117606768"
ORIGIN      
        1 magqdpkmqk eaadqnfdym fklliignss vgktsflfry addsftsafv stvgidfkvk
       61 tvfrhdkrvk lqiwdtagqe ryrtittayy rgamgfilmy ditneesfns vqdwvtqikt
      121 yscdnaqvil vgnkcdmede rvisfergkq ladqlgvqff etsakenini ktvfeqlvdi
      181 icdkmsesld ndptllaggm gqakgqrltd qpanqssdnc nc
//
LOCUS       XP_034185549             109 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606769 [Osmia lignaria lignaria].
ACCESSION   XP_034185549
VERSION     XP_034185549.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329658.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..109
                     /product="uncharacterized protein LOC117606769"
                     /calculated_mol_wt=12718
     CDS             1..109
                     /gene="LOC117606769"
                     /coded_by="XM_034329658.2:554..883"
                     /db_xref="GeneID:117606769"
ORIGIN      
        1 mshssfhnsk dsfvrerqil epeassedhi vkrsqsgrqi kelnlnlrkc qsvdgsvkkl
       61 hkrrrrvrpr slkvpqvpek ivnivnilqh enflevvdrf fkpkvtkqp
//
LOCUS       XP_034194832             106 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611051 [Osmia lignaria lignaria].
ACCESSION   XP_034194832
VERSION     XP_034194832.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338941.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="uncharacterized protein LOC117611051"
                     /calculated_mol_wt=11684
     CDS             1..106
                     /gene="LOC117611051"
                     /coded_by="XM_034338941.2:95..415"
                     /db_xref="GeneID:117611051"
ORIGIN      
        1 mkvnqgkrsr rptrrggvkq raralikrqi qlvqkeletr fppsgpsshp gepcyspvse
       61 asticlgprs pqlidlteep elesvnvaet neipaeilai ifnlqe
//
LOCUS       XP_034185568            1769 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain containing 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185568
VERSION     XP_034185568.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329677.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1769
                     /product="SET domain containing 2 isoform X1"
                     /calculated_mol_wt=201975
     Region          762..815
                     /region_name="AWS"
                     /note="associated with SET domains; smart00570"
                     /db_xref="CDD:197795"
     Region          815..956
                     /region_name="SET_SETD2"
                     /note="SET domain (including post-SET domain) found in SET
                     domain-containing protein 2 (SETD2) and similar proteins;
                     cd19172"
                     /db_xref="CDD:380949"
     Site            order(826..828,845,855,859,863,868..874,891..895,902..904,
                     919,930..934,936..940,942..946,955)
                     /site_type="active"
                     /db_xref="CDD:380949"
     Site            order(826..828,868..871,891..895,932,942..946,955)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380949"
     Site            order(845,855,859,870..874,902..904,919,930..934)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380949"
     Site            order(897,944,946,951)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380949"
     Region          1482..1511
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          1674..1762
                     /region_name="SRI"
                     /note="SRI (Set2 Rpb1 interacting) domain; pfam08236"
                     /db_xref="CDD:462404"
     CDS             1..1769
                     /gene="Set2"
                     /coded_by="XM_034329677.2:532..5841"
                     /db_xref="GeneID:117606783"
ORIGIN      
        1 mtrgihgikm arrrktemks svkssikyvp vrkssrqiak kqseekkvng eilqisylnn
       61 sedgrsatey pnqvlpkknq nsvrkhnkhk ngsnsnvlai neeyaystts qykkdssesd
      121 sqdgvdiiid qseelspedv qqvvvgnpsk eiltevdsne ieiwpedvie etiiceemev
      181 kddvkdnnys lkeednamvk evllvnnpsl ekknfleytv iqnensaasm liecdsints
      241 snecccdven kmcalhkskt dnsisglcte vpiicidknn eytrnsktil ndqeelqvnn
      301 gtsvkkshen lskdefckid nklaemqine sskeiqklvn dlsnienims plqitdniek
      361 vipmsvddes sssildkmeq lhqsnnsasl ldtidkntie lnksdscndk vectnqnvlk
      421 lkvlqdkkne kiqrkpmnsq qineeygdse adskldgedt klssssdnsn dtllsmnnck
      481 lteldpkcnn ssndidkrse frsrsgstdt tgsesgsnss gvrrssrirs iglmkqrsrg
      541 rglvtkpnid vskpivqper ekiinnncsi snevkidntt epqsdkennv nktantfdsv
      601 tslatncptt gydsdsskpv kvksrwrrss elemggsstg avsaltigsl lpnattnise
      661 tgssstalkf ttgsltdisc sdsgsgstvt ntqsnvdteq tketlitntt nivqipktvg
      721 akiflpmvpe ikdremeerl sqfeylhenl ylterytnke tkrmvcdcfl tdeeiergel
      781 gcgedclnrl lmiecgprci vgdrctnkrf qnceyakcev frtekkgfgl raivdmlage
      841 fimeyvgevv dpkdfrrrak eyskdknkhy yfmalksdqi idatmkgnvs rfinhscdpn
      901 setqkwtvng elrigffnkk fiaageeitf dyhfqrygke aqkcfceapn crgwigetpe
      961 eekekiekke krdkdmrkkk gekkqadyme dedleeeidk lclgglknra htltlsrlmv
     1021 rsrelehrtr llrliqsgeq pcrrlfldyh glrliwsyvm dvstndseea qqfrlevlkt
     1081 lntlpipnkt mlmdskiynv vekwsrrlyf spsgdspedd qekvkgncee lqsnydvnql
     1141 tdtekqenni enseikldng drnlipelas sllaewsnlk evfripkker ieqmkehere
     1201 adrgyreele keekrgtsyd rhrsdrygrn etekrgdrrr grespeseht rtkdkrveer
     1261 sslvpiprmt kyerrqlfal kvakeeeerq rrqqqeswqd hetrclalsi dphttamvdp
     1321 qtgypvfynp sigqwqqypt qdseisqqct piyvggaqav aghsqsgivs qnshvlppsi
     1381 pseissgmss sipsgvppvi ytisqtpvfn qtttayslls hchqqypegq lslpnsivhs
     1441 gtsvtiqsqs lyehiekqqq sdqqfqqstf rqaqpevppi dlppkwksat dargrtyyyh
     1501 vkerisqwlp pppdhigvqp dssstsesse essssnedee divdednned srneelslnn
     1561 ilveksldgs khnackksaf hltgntgllp egkkrreglv qeriisprre edrmdhkthk
     1621 gikeklrrqk erakfkehvd kmrrhrrnnk skshsrhsls klqspstdls tmserkikdt
     1681 frinmanvmv hflnpyrknd ckqgritnte dfkhlarklt hfvlakelkh cksvdelqcn
     1741 envkhkakdf vrkymskfga vyqkstded
//
LOCUS       XP_034185567            1769 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain containing 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185567
VERSION     XP_034185567.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329676.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1769
                     /product="SET domain containing 2 isoform X1"
                     /calculated_mol_wt=201975
     Region          762..815
                     /region_name="AWS"
                     /note="associated with SET domains; smart00570"
                     /db_xref="CDD:197795"
     Region          815..956
                     /region_name="SET_SETD2"
                     /note="SET domain (including post-SET domain) found in SET
                     domain-containing protein 2 (SETD2) and similar proteins;
                     cd19172"
                     /db_xref="CDD:380949"
     Site            order(826..828,845,855,859,863,868..874,891..895,902..904,
                     919,930..934,936..940,942..946,955)
                     /site_type="active"
                     /db_xref="CDD:380949"
     Site            order(826..828,868..871,891..895,932,942..946,955)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380949"
     Site            order(845,855,859,870..874,902..904,919,930..934)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380949"
     Site            order(897,944,946,951)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380949"
     Region          1482..1511
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          1674..1762
                     /region_name="SRI"
                     /note="SRI (Set2 Rpb1 interacting) domain; pfam08236"
                     /db_xref="CDD:462404"
     CDS             1..1769
                     /gene="Set2"
                     /coded_by="XM_034329676.2:427..5736"
                     /db_xref="GeneID:117606783"
ORIGIN      
        1 mtrgihgikm arrrktemks svkssikyvp vrkssrqiak kqseekkvng eilqisylnn
       61 sedgrsatey pnqvlpkknq nsvrkhnkhk ngsnsnvlai neeyaystts qykkdssesd
      121 sqdgvdiiid qseelspedv qqvvvgnpsk eiltevdsne ieiwpedvie etiiceemev
      181 kddvkdnnys lkeednamvk evllvnnpsl ekknfleytv iqnensaasm liecdsints
      241 snecccdven kmcalhkskt dnsisglcte vpiicidknn eytrnsktil ndqeelqvnn
      301 gtsvkkshen lskdefckid nklaemqine sskeiqklvn dlsnienims plqitdniek
      361 vipmsvddes sssildkmeq lhqsnnsasl ldtidkntie lnksdscndk vectnqnvlk
      421 lkvlqdkkne kiqrkpmnsq qineeygdse adskldgedt klssssdnsn dtllsmnnck
      481 lteldpkcnn ssndidkrse frsrsgstdt tgsesgsnss gvrrssrirs iglmkqrsrg
      541 rglvtkpnid vskpivqper ekiinnncsi snevkidntt epqsdkennv nktantfdsv
      601 tslatncptt gydsdsskpv kvksrwrrss elemggsstg avsaltigsl lpnattnise
      661 tgssstalkf ttgsltdisc sdsgsgstvt ntqsnvdteq tketlitntt nivqipktvg
      721 akiflpmvpe ikdremeerl sqfeylhenl ylterytnke tkrmvcdcfl tdeeiergel
      781 gcgedclnrl lmiecgprci vgdrctnkrf qnceyakcev frtekkgfgl raivdmlage
      841 fimeyvgevv dpkdfrrrak eyskdknkhy yfmalksdqi idatmkgnvs rfinhscdpn
      901 setqkwtvng elrigffnkk fiaageeitf dyhfqrygke aqkcfceapn crgwigetpe
      961 eekekiekke krdkdmrkkk gekkqadyme dedleeeidk lclgglknra htltlsrlmv
     1021 rsrelehrtr llrliqsgeq pcrrlfldyh glrliwsyvm dvstndseea qqfrlevlkt
     1081 lntlpipnkt mlmdskiynv vekwsrrlyf spsgdspedd qekvkgncee lqsnydvnql
     1141 tdtekqenni enseikldng drnlipelas sllaewsnlk evfripkker ieqmkehere
     1201 adrgyreele keekrgtsyd rhrsdrygrn etekrgdrrr grespeseht rtkdkrveer
     1261 sslvpiprmt kyerrqlfal kvakeeeerq rrqqqeswqd hetrclalsi dphttamvdp
     1321 qtgypvfynp sigqwqqypt qdseisqqct piyvggaqav aghsqsgivs qnshvlppsi
     1381 pseissgmss sipsgvppvi ytisqtpvfn qtttayslls hchqqypegq lslpnsivhs
     1441 gtsvtiqsqs lyehiekqqq sdqqfqqstf rqaqpevppi dlppkwksat dargrtyyyh
     1501 vkerisqwlp pppdhigvqp dssstsesse essssnedee divdednned srneelslnn
     1561 ilveksldgs khnackksaf hltgntgllp egkkrreglv qeriisprre edrmdhkthk
     1621 gikeklrrqk erakfkehvd kmrrhrrnnk skshsrhsls klqspstdls tmserkikdt
     1681 frinmanvmv hflnpyrknd ckqgritnte dfkhlarklt hfvlakelkh cksvdelqcn
     1741 envkhkakdf vrkymskfga vyqkstded
//
LOCUS       XP_034185569            1760 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain containing 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185569
VERSION     XP_034185569.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329678.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1760
                     /product="SET domain containing 2 isoform X2"
                     /calculated_mol_wt=200981
     Region          753..806
                     /region_name="AWS"
                     /note="associated with SET domains; smart00570"
                     /db_xref="CDD:197795"
     Region          806..947
                     /region_name="SET_SETD2"
                     /note="SET domain (including post-SET domain) found in SET
                     domain-containing protein 2 (SETD2) and similar proteins;
                     cd19172"
                     /db_xref="CDD:380949"
     Site            order(817..819,836,846,850,854,859..865,882..886,893..895,
                     910,921..925,927..931,933..937,946)
                     /site_type="active"
                     /db_xref="CDD:380949"
     Site            order(817..819,859..862,882..886,923,933..937,946)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380949"
     Site            order(836,846,850,861..865,893..895,910,921..925)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380949"
     Site            order(888,935,937,942)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380949"
     Region          1473..1502
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          1665..1753
                     /region_name="SRI"
                     /note="SRI (Set2 Rpb1 interacting) domain; pfam08236"
                     /db_xref="CDD:462404"
     CDS             1..1760
                     /gene="Set2"
                     /coded_by="XM_034329678.2:177..5459"
                     /db_xref="GeneID:117606783"
ORIGIN      
        1 marrrktemk ssvkssikyv pvrkssrqia kkqseekkvn geilqisyln nsedgrsate
       61 ypnqvlpkkn qnsvrkhnkh kngsnsnvla ineeyaystt sqykkdsses dsqdgvdiii
      121 dqseelsped vqqvvvgnps keiltevdsn eieiwpedvi eetiiceeme vkddvkdnny
      181 slkeednamv kevllvnnps lekknfleyt viqnensaas mliecdsint ssnecccdve
      241 nkmcalhksk tdnsisglct evpiicidkn neytrnskti lndqeelqvn ngtsvkkshe
      301 nlskdefcki dnklaemqin esskeiqklv ndlsnienim splqitdnie kvipmsvdde
      361 ssssildkme qlhqsnnsas lldtidknti elnksdscnd kvectnqnvl klkvlqdkkn
      421 ekiqrkpmns qqineeygds eadskldged tklssssdns ndtllsmnnc klteldpkcn
      481 nssndidkrs efrsrsgstd ttgsesgsns sgvrrssrir siglmkqrsr grglvtkpni
      541 dvskpivqpe rekiinnncs isnevkidnt tepqsdkenn vnktantfds vtslatncpt
      601 tgydsdsskp vkvksrwrrs selemggsst gavsaltigs llpnattnis etgssstalk
      661 fttgsltdis csdsgsgstv tntqsnvdte qtketlitnt tnivqipktv gakiflpmvp
      721 eikdremeer lsqfeylhen lylterytnk etkrmvcdcf ltdeeierge lgcgedclnr
      781 llmiecgprc ivgdrctnkr fqnceyakce vfrtekkgfg lraivdmlag efimeyvgev
      841 vdpkdfrrra keyskdknkh yyfmalksdq iidatmkgnv srfinhscdp nsetqkwtvn
      901 gelrigffnk kfiaageeit fdyhfqrygk eaqkcfceap ncrgwigetp eeekekiekk
      961 ekrdkdmrkk kgekkqadym ededleeeid klclgglknr ahtltlsrlm vrsrelehrt
     1021 rllrliqsge qpcrrlfldy hglrliwsyv mdvstndsee aqqfrlevlk tlntlpipnk
     1081 tmlmdskiyn vvekwsrrly fspsgdsped dqekvkgnce elqsnydvnq ltdtekqenn
     1141 ienseikldn gdrnlipela ssllaewsnl kevfripkke rieqmkeher eadrgyreel
     1201 ekeekrgtsy drhrsdrygr netekrgdrr rgrespeseh trtkdkrvee rsslvpiprm
     1261 tkyerrqlfa lkvakeeeer qrrqqqeswq dhetrclals idphttamvd pqtgypvfyn
     1321 psigqwqqyp tqdseisqqc tpiyvggaqa vaghsqsgiv sqnshvlpps ipseissgms
     1381 ssipsgvppv iytisqtpvf nqtttaysll shchqqypeg qlslpnsivh sgtsvtiqsq
     1441 slyehiekqq qsdqqfqqst frqaqpevpp idlppkwksa tdargrtyyy hvkerisqwl
     1501 ppppdhigvq pdssstsess eessssnede edivdednne dsrneelsln nilveksldg
     1561 skhnackksa fhltgntgll pegkkrregl vqeriisprr eedrmdhkth kgikeklrrq
     1621 kerakfkehv dkmrrhrrnn kskshsrhsl sklqspstdl stmserkikd tfrinmanvm
     1681 vhflnpyrkn dckqgritnt edfkhlarkl thfvlakelk hcksvdelqc nenvkhkakd
     1741 fvrkymskfg avyqkstded
//
LOCUS       XP_034185570            1760 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain containing 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185570
VERSION     XP_034185570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329679.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1760
                     /product="SET domain containing 2 isoform X2"
                     /calculated_mol_wt=200981
     Region          753..806
                     /region_name="AWS"
                     /note="associated with SET domains; smart00570"
                     /db_xref="CDD:197795"
     Region          806..947
                     /region_name="SET_SETD2"
                     /note="SET domain (including post-SET domain) found in SET
                     domain-containing protein 2 (SETD2) and similar proteins;
                     cd19172"
                     /db_xref="CDD:380949"
     Site            order(817..819,836,846,850,854,859..865,882..886,893..895,
                     910,921..925,927..931,933..937,946)
                     /site_type="active"
                     /db_xref="CDD:380949"
     Site            order(817..819,859..862,882..886,923,933..937,946)
                     /site_type="other"
                     /note="SAM binding site"
                     /db_xref="CDD:380949"
     Site            order(836,846,850,861..865,893..895,910,921..925)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380949"
     Site            order(888,935,937,942)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380949"
     Region          1473..1502
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          1665..1753
                     /region_name="SRI"
                     /note="SRI (Set2 Rpb1 interacting) domain; pfam08236"
                     /db_xref="CDD:462404"
     CDS             1..1760
                     /gene="Set2"
                     /coded_by="XM_034329679.2:165..5447"
                     /db_xref="GeneID:117606783"
ORIGIN      
        1 marrrktemk ssvkssikyv pvrkssrqia kkqseekkvn geilqisyln nsedgrsate
       61 ypnqvlpkkn qnsvrkhnkh kngsnsnvla ineeyaystt sqykkdsses dsqdgvdiii
      121 dqseelsped vqqvvvgnps keiltevdsn eieiwpedvi eetiiceeme vkddvkdnny
      181 slkeednamv kevllvnnps lekknfleyt viqnensaas mliecdsint ssnecccdve
      241 nkmcalhksk tdnsisglct evpiicidkn neytrnskti lndqeelqvn ngtsvkkshe
      301 nlskdefcki dnklaemqin esskeiqklv ndlsnienim splqitdnie kvipmsvdde
      361 ssssildkme qlhqsnnsas lldtidknti elnksdscnd kvectnqnvl klkvlqdkkn
      421 ekiqrkpmns qqineeygds eadskldged tklssssdns ndtllsmnnc klteldpkcn
      481 nssndidkrs efrsrsgstd ttgsesgsns sgvrrssrir siglmkqrsr grglvtkpni
      541 dvskpivqpe rekiinnncs isnevkidnt tepqsdkenn vnktantfds vtslatncpt
      601 tgydsdsskp vkvksrwrrs selemggsst gavsaltigs llpnattnis etgssstalk
      661 fttgsltdis csdsgsgstv tntqsnvdte qtketlitnt tnivqipktv gakiflpmvp
      721 eikdremeer lsqfeylhen lylterytnk etkrmvcdcf ltdeeierge lgcgedclnr
      781 llmiecgprc ivgdrctnkr fqnceyakce vfrtekkgfg lraivdmlag efimeyvgev
      841 vdpkdfrrra keyskdknkh yyfmalksdq iidatmkgnv srfinhscdp nsetqkwtvn
      901 gelrigffnk kfiaageeit fdyhfqrygk eaqkcfceap ncrgwigetp eeekekiekk
      961 ekrdkdmrkk kgekkqadym ededleeeid klclgglknr ahtltlsrlm vrsrelehrt
     1021 rllrliqsge qpcrrlfldy hglrliwsyv mdvstndsee aqqfrlevlk tlntlpipnk
     1081 tmlmdskiyn vvekwsrrly fspsgdsped dqekvkgnce elqsnydvnq ltdtekqenn
     1141 ienseikldn gdrnlipela ssllaewsnl kevfripkke rieqmkeher eadrgyreel
     1201 ekeekrgtsy drhrsdrygr netekrgdrr rgrespeseh trtkdkrvee rsslvpiprm
     1261 tkyerrqlfa lkvakeeeer qrrqqqeswq dhetrclals idphttamvd pqtgypvfyn
     1321 psigqwqqyp tqdseisqqc tpiyvggaqa vaghsqsgiv sqnshvlpps ipseissgms
     1381 ssipsgvppv iytisqtpvf nqtttaysll shchqqypeg qlslpnsivh sgtsvtiqsq
     1441 slyehiekqq qsdqqfqqst frqaqpevpp idlppkwksa tdargrtyyy hvkerisqwl
     1501 ppppdhigvq pdssstsess eessssnede edivdednne dsrneelsln nilveksldg
     1561 skhnackksa fhltgntgll pegkkrregl vqeriisprr eedrmdhkth kgikeklrrq
     1621 kerakfkehv dkmrrhrrnn kskshsrhsl sklqspstdl stmserkikd tfrinmanvm
     1681 vhflnpyrkn dckqgritnt edfkhlarkl thfvlakelk hcksvdelqc nenvkhkakd
     1741 fvrkymskfg avyqkstded
//
LOCUS       XP_034185572            1047 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase N [Osmia lignaria lignaria].
ACCESSION   XP_034185572
VERSION     XP_034185572.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329681.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185572.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1047
                     /product="aminopeptidase N"
                     /calculated_mol_wt=120592
     Region          109..627
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     Region          704..1003
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..1047
                     /gene="LOC117606784"
                     /coded_by="XM_034329681.2:864..4007"
                     /db_xref="GeneID:117606784"
ORIGIN      
        1 msrgsisswq dygrrssqgi pqfsggpvef mtsdgmeykr gdgwflsytk iagivvvfvi
       61 gvvaagflgw yinslpkkkp ydtldllnde iedidsvset lispsvnplk yrlqltpiin
      121 insvsgivgr viiefrvnda tsklslnarn isvenyklll ldpekgkeaf rrrldndeak
      181 yssqennrtl avramysven ddlvtvsdne ttlfekntet ssinndsfry pnqnkdpnls
      241 dsnsfseiei teyriddekd ihtihlanql kegiyfleid yqivinenaf fitnccssee
      301 erwfmgtklk rsgarylfpv fddtlhksif svsvihfres rvlsnmplrt lrdtsnvsvv
      361 idtfdesppl sphnlaflmg hiesigvtfa gnsdvvvtfw gesnrqsqgi yvfdkfnqav
      421 tnlidvfsmp yplpkldvvg lpsgidenma npgliemkqs lfyatenssm alktnalrvl
      481 vdlleqqwts eftnanrtda wifegsmiyf qqeivrkids lsidffvgdi qshsmeidey
      541 sisrplhenv nhrslhsldd eyakgaclir mlhgaindta frngykklis rwkyntteva
      601 dflnfmqeea sglpsemsle eamnswifqg gyplitvirn yekgsailyq eqftldrple
      661 siskfwhvpl dyiqedgdws sptkiwfqse pkikldninl neswilfnvn ktgyyrvhyd
      721 ernwkllkmv lqdnhelfpp etrasliddv fslaavglmk yeavfdfiky mqtkerhylp
      781 wtvlmrhtfr lnrllyetta fsdfqvfmik fvsplynevg sslkegsqlp lvavkmacmf
      841 ehpqclnwsn nvfenikndt elyeivpshv retfyctvar ygtrrewsyf teritltede
      901 eekkrllsay acfqapwilq silneivhee lfqedevavi lksfprnpaa aqaasrfvra
      961 nwheitqrfp gsysllrtfv lsmingvtte qdledlqlfr ennyesmkga rygaalvean
     1021 gnfgtlwlks flpefekllk eevidea
//
LOCUS       XP_034185573            1047 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase N [Osmia lignaria lignaria].
ACCESSION   XP_034185573
VERSION     XP_034185573.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329682.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185573.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1047
                     /product="aminopeptidase N"
                     /calculated_mol_wt=120592
     Region          109..627
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     Region          704..1003
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..1047
                     /gene="LOC117606784"
                     /coded_by="XM_034329682.2:496..3639"
                     /db_xref="GeneID:117606784"
ORIGIN      
        1 msrgsisswq dygrrssqgi pqfsggpvef mtsdgmeykr gdgwflsytk iagivvvfvi
       61 gvvaagflgw yinslpkkkp ydtldllnde iedidsvset lispsvnplk yrlqltpiin
      121 insvsgivgr viiefrvnda tsklslnarn isvenyklll ldpekgkeaf rrrldndeak
      181 yssqennrtl avramysven ddlvtvsdne ttlfekntet ssinndsfry pnqnkdpnls
      241 dsnsfseiei teyriddekd ihtihlanql kegiyfleid yqivinenaf fitnccssee
      301 erwfmgtklk rsgarylfpv fddtlhksif svsvihfres rvlsnmplrt lrdtsnvsvv
      361 idtfdesppl sphnlaflmg hiesigvtfa gnsdvvvtfw gesnrqsqgi yvfdkfnqav
      421 tnlidvfsmp yplpkldvvg lpsgidenma npgliemkqs lfyatenssm alktnalrvl
      481 vdlleqqwts eftnanrtda wifegsmiyf qqeivrkids lsidffvgdi qshsmeidey
      541 sisrplhenv nhrslhsldd eyakgaclir mlhgaindta frngykklis rwkyntteva
      601 dflnfmqeea sglpsemsle eamnswifqg gyplitvirn yekgsailyq eqftldrple
      661 siskfwhvpl dyiqedgdws sptkiwfqse pkikldninl neswilfnvn ktgyyrvhyd
      721 ernwkllkmv lqdnhelfpp etrasliddv fslaavglmk yeavfdfiky mqtkerhylp
      781 wtvlmrhtfr lnrllyetta fsdfqvfmik fvsplynevg sslkegsqlp lvavkmacmf
      841 ehpqclnwsn nvfenikndt elyeivpshv retfyctvar ygtrrewsyf teritltede
      901 eekkrllsay acfqapwilq silneivhee lfqedevavi lksfprnpaa aqaasrfvra
      961 nwheitqrfp gsysllrtfv lsmingvtte qdledlqlfr ennyesmkga rygaalvean
     1021 gnfgtlwlks flpefekllk eevidea
//
LOCUS       XP_034185574             966 aa            linear   INV 03-SEP-2025
DEFINITION  aminopeptidase N [Osmia lignaria lignaria].
ACCESSION   XP_034185574
VERSION     XP_034185574.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329683.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..966
                     /product="aminopeptidase N"
                     /calculated_mol_wt=112258
     Region          69..512
                     /region_name="GluZincin"
                     /note="Gluzincin Peptidase family (thermolysin-like
                     proteinases, TLPs) which includes peptidases M1, M2, M3,
                     M4, M13, M32 and M36 (fungalysins); cl14813"
                     /db_xref="CDD:472708"
     Region          595..897
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..966
                     /gene="LOC117606785"
                     /coded_by="XM_034329683.2:108..3008"
                     /db_xref="GeneID:117606785"
ORIGIN      
        1 mrlrtcwtri akrtmrylaa itvlflilal phyasaspav tsrfkrsmdl neleyhrilh
       61 rlpqevvats yhlelqpfvg ndkfngrvri nvtwtdtsdt itlnadpdih iskysvrite
      121 aaseerekgl pmmdvnvarv tlpdwqkpwy tihleqmlkk gsscevdisf vgnltsnvhc
      181 glyrkeypdh pyvatyfrpn rahqvfpcmd eppykatfkl svlrpvnmta lsntplelta
      241 asdnqnfvwd hfettpkmst yqlallvsdf tsispneein emdgrrveik vwapkkhlds
      301 lehvpdkivw imnylqeyfn ssivlpkldi valpwlhdnp tdnwglmffg tnhlnspsiw
      361 tvtyeliyqw igqyitpfsw sdapvskaln sflasmttvd inpdemegrw pmtmlysfyy
      421 efgkykpfsk aagitneata sktelvfrmf nillgkemfr kgvrnfirqn seenlrtffa
      481 ddiyshlndi anetnilpeg rtvssiaglw itrdrlplvt virdyetktm tlsqkvnlre
      541 apraatpkvs yqwdipivmm seeklefhkp yplwlpkgyk pknltvpdit dedhfiivnp
      601 eeigmfpvny dscnwkmliq ylrgpnreki pvltrakllh dswnlaysge lcfgialnmt
      661 lflqeerchv vwepifmmid hidrriegse vypkfqayir tllkplymel keneqageps
      721 wksrmrgmak nflcragyep ciiearnqyq rwlrgedqda rnsandficp llkwgteeew
      781 efglqrimnv skyermdlks lagctkdank ierllnvtil eqnenfsdsd iqlifvtlsg
      841 gaagyrtlfn flidnwdtvk qrfedkhylw ssiielatst ystqqgvdlv eklyeshrdh
      901 vsknlpiqka ftadrkqwte knlpvidawl senlpkeele atqsstattm vmpvkhlnil
      961 lnhlkl
//
LOCUS       XP_034185575             570 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185575
VERSION     XP_034185575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="nicotinic acetylcholine receptor alpha4 isoform
                     X1"
                     /calculated_mol_wt=64887
     Region          14..538
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          29..247
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Site            order(65,67,73..74,81,103,105,114..115,117,119..120,124,
                     126..127,131..133,135,145,147,149,153,175,244)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          154..168
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     CDS             1..570
                     /gene="nAChRalpha4"
                     /coded_by="XM_034329684.2:291..2003"
                     /db_xref="GeneID:117606786"
ORIGIN      
        1 mmppiigetl rvwflsalvv hgavagnpda krlyddllsn ynklvrpvvn tsdvlrvcik
       61 lklsqlidvn lknqimttnl wveqswydyk lrwepkeygg vhmlhvpsdh iwrpdivlyn
      121 nadgnfevtl atkatiyhqg lvewkppaiy kssceidvey fpfdeqtcvl kfgswtydgf
      181 kvdlrhmdek sgsnvvdvgv dlsefymsve wdilevpavr nekfytccde pylditfnit
      241 mrrktlfytv niiipcmgis fltvltfylp sdsgekvtls isilislhvf fllvveiipp
      301 tslvvpllgk ylifamilvs isicvtvvvl nvhfrspqth kmapwvkrvf ihilprllvm
      361 rrpqykfean ryssgrvlmr tvrgkektcy ypyhpstqen seehltpkrf hsraaskedl
      421 spssladgar fggsclihgp plpplplhte cedlsvsgea gieevkspvl rsppafshsr
      481 cppeihksci cvrfiaehtk mledstkvke dwkyvamvld rlflwiftla vlvgtagiil
      541 qaptlyddrv pidkkfsefa tttvvncppq
//
LOCUS       XP_076545849             570 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545849
VERSION     XP_076545849.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689734.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="nicotinic acetylcholine receptor alpha4 isoform
                     X1"
                     /calculated_mol_wt=64887
     Region          14..538
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          29..247
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Site            order(65,67,73..74,81,103,105,114..115,117,119..120,124,
                     126..127,131..133,135,145,147,149,153,175,244)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          154..168
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     CDS             1..570
                     /gene="nAChRalpha4"
                     /coded_by="XM_076689734.1:291..2003"
                     /db_xref="GeneID:117606786"
ORIGIN      
        1 mmppiigetl rvwflsalvv hgavagnpda krlyddllsn ynklvrpvvn tsdvlrvcik
       61 lklsqlidvn lknqimttnl wveqswydyk lrwepkeygg vhmlhvpsdh iwrpdivlyn
      121 nadgnfevtl atkatiyhqg lvewkppaiy kssceidvey fpfdeqtcvl kfgswtydgf
      181 kvdlrhmdek sgsnvvdvgv dlsefymsve wdilevpavr nekfytccde pylditfnit
      241 mrrktlfytv niiipcmgis fltvltfylp sdsgekvtls isilislhvf fllvveiipp
      301 tslvvpllgk ylifamilvs isicvtvvvl nvhfrspqth kmapwvkrvf ihilprllvm
      361 rrpqykfean ryssgrvlmr tvrgkektcy ypyhpstqen seehltpkrf hsraaskedl
      421 spssladgar fggsclihgp plpplplhte cedlsvsgea gieevkspvl rsppafshsr
      481 cppeihksci cvrfiaehtk mledstkvke dwkyvamvld rlflwiftla vlvgtagiil
      541 qaptlyddrv pidkkfsefa tttvvncppq
//
LOCUS       XP_076545850             349 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545850
VERSION     XP_076545850.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689735.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..349
                     /product="nicotinic acetylcholine receptor alpha4 isoform
                     X2"
                     /calculated_mol_wt=40097
     Region          29..247
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Site            order(65,67,73..74,81,103,105,114..115,117,119..120,124,
                     126..127,131..133,135,145,147,149,153,175,244)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          154..168
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     Region          246..>322
                     /region_name="LGIC_TM_nAChR"
                     /note="transmembrane domain of nicotinic acetylcholine
                     receptor (nAChR); cd19064"
                     /db_xref="CDD:349866"
     Region          246..270
                     /region_name="TM1 helix"
                     /note="TM1 helix [structural motif]"
                     /db_xref="CDD:349866"
     Region          278..299
                     /region_name="TM2 helix"
                     /note="TM2 helix [structural motif]"
                     /db_xref="CDD:349866"
     CDS             1..349
                     /gene="nAChRalpha4"
                     /coded_by="XM_076689735.1:292..1341"
                     /db_xref="GeneID:117606786"
ORIGIN      
        1 mmppiigetl rvwflsalvv hgavagnpda krlyddllsn ynklvrpvvn tsdvlrvcik
       61 lklsqlidvn lknqimttnl wveqswydyk lrwepkeygg vhmlhvpsdh iwrpdivlyn
      121 nadgnfevtl atkatiyhqg lvewkppaiy kssceidvey fpfdeqtcvl kfgswtydgf
      181 kvdlrhmdek sgsnvvdvgv dlsefymsve wdilevpavr nekfytccde pylditfnit
      241 mrrktlfytv niiipcmgis fltvltfylp sdsgekvtls isilislhvf fllvveiipp
      301 tslvvpllgk ylifamilvs iswqdwkflp aggrrrkcqe tsvcqprir
//
LOCUS       XP_076545851             349 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545851
VERSION     XP_076545851.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689736.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..349
                     /product="nicotinic acetylcholine receptor alpha4 isoform
                     X2"
                     /calculated_mol_wt=40097
     Region          29..247
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Site            order(65,67,73..74,81,103,105,114..115,117,119..120,124,
                     126..127,131..133,135,145,147,149,153,175,244)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          154..168
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     Region          246..>322
                     /region_name="LGIC_TM_nAChR"
                     /note="transmembrane domain of nicotinic acetylcholine
                     receptor (nAChR); cd19064"
                     /db_xref="CDD:349866"
     Region          246..270
                     /region_name="TM1 helix"
                     /note="TM1 helix [structural motif]"
                     /db_xref="CDD:349866"
     Region          278..299
                     /region_name="TM2 helix"
                     /note="TM2 helix [structural motif]"
                     /db_xref="CDD:349866"
     CDS             1..349
                     /gene="nAChRalpha4"
                     /coded_by="XM_076689736.1:292..1341"
                     /db_xref="GeneID:117606786"
ORIGIN      
        1 mmppiigetl rvwflsalvv hgavagnpda krlyddllsn ynklvrpvvn tsdvlrvcik
       61 lklsqlidvn lknqimttnl wveqswydyk lrwepkeygg vhmlhvpsdh iwrpdivlyn
      121 nadgnfevtl atkatiyhqg lvewkppaiy kssceidvey fpfdeqtcvl kfgswtydgf
      181 kvdlrhmdek sgsnvvdvgv dlsefymsve wdilevpavr nekfytccde pylditfnit
      241 mrrktlfytv niiipcmgis fltvltfylp sdsgekvtls isilislhvf fllvveiipp
      301 tslvvpllgk ylifamilvs iswqdwkflp aggrrrkcqe tsvcqprir
//
LOCUS       XP_076545852             384 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha4 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545852
VERSION     XP_076545852.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689737.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="nicotinic acetylcholine receptor alpha4 isoform
                     X3"
                     /calculated_mol_wt=43315
     Region          <1..61
                     /region_name="LGIC_ECD"
                     /note="extracellular domain (ECD) of Cys-loop
                     neurotransmitter-gated ion channels (also known as
                     ligand-gated ion channel (LGIC)); cl28912"
                     /db_xref="CDD:475126"
     Region          66..352
                     /region_name="Neur_chan_memb"
                     /note="Neurotransmitter-gated ion-channel transmembrane
                     region; pfam02932"
                     /db_xref="CDD:460753"
     CDS             1..384
                     /gene="nAChRalpha4"
                     /coded_by="XM_076689737.1:30..1184"
                     /db_xref="GeneID:117606786"
ORIGIN      
        1 mdeksgsnvv dvgvdlsefy msvewdilev pavrnekfyt ccdepyldit fnitmrrktl
       61 fytvniiipc mgisfltvlt fylpsdsgek vtlsisilis lhvffllvve iipptslvvp
      121 llgkylifam ilvsisicvt vvvlnvhfrs pqthkmapwv krvfihilpr llvmrrpqyk
      181 feanryssgr vlmrtvrgke ktcyypyhps tqenseehlt pkrfhsraas kedlspssla
      241 dgarfggscl ihgpplpplp lhtecedlsv sgeagieevk spvlrsppaf shsrcppeih
      301 kscicvrfia ehtkmledst kvkedwkyva mvldrlflwi ftlavlvgta giilqaptly
      361 ddrvpidkkf sefatttvvn cppq
//
LOCUS       XP_034185578             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606787 [Osmia lignaria lignaria].
ACCESSION   XP_034185578
VERSION     XP_034185578.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC117606787"
                     /calculated_mol_wt=56505
     Region          65..134
                     /region_name="DUF4792"
                     /note="Domain of unknown function (DUF4792); pfam16040"
                     /db_xref="CDD:464988"
     Region          379..487
                     /region_name="DUF4793"
                     /note="Domain of unknown function (DUF4793); pfam16041"
                     /db_xref="CDD:464989"
     CDS             1..490
                     /gene="LOC117606787"
                     /coded_by="XM_034329687.2:133..1605"
                     /db_xref="GeneID:117606787"
ORIGIN      
        1 mrgikrilvf ctlttivpil flitplylrh nfygnvayav tdsdileitd gistvfcseh
       61 vlqmnrtfna fqmahkpeit sykkhirlkk nmtlpddtle ywgfyllegs svalsvcsrf
      121 egasilvvkg ernlrtcgml ehnneeiven iflpeakqqv kvtfksdtes isnnsiiqvd
      181 hndtlnnttn kffrnitskl eenhtdknat nvhsainsnt lsneeeklrk lvwdtkmymq
      241 qhmkspkepi vhnarklrha kkkqykkqik ekeknmlegr rrtrrdieng skvkekeifk
      301 krtkrnqdlv kpflldqgvk hggnavknlt ngdddssvss fenelfkcyg gsilisqefp
      361 pseqctnvty llngkhmqat hnviengyyy yifysdndiv sndiyalfdi ykpifhyeni
      421 tkscinqtkc sfsinllssd rviveiptkd gleyeiddis lllsvchprm evyvifpiav
      481 lffilacafm
//
LOCUS       XP_034185580             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606787 [Osmia lignaria lignaria].
ACCESSION   XP_034185580
VERSION     XP_034185580.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329689.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC117606787"
                     /calculated_mol_wt=56505
     Region          65..134
                     /region_name="DUF4792"
                     /note="Domain of unknown function (DUF4792); pfam16040"
                     /db_xref="CDD:464988"
     Region          379..487
                     /region_name="DUF4793"
                     /note="Domain of unknown function (DUF4793); pfam16041"
                     /db_xref="CDD:464989"
     CDS             1..490
                     /gene="LOC117606787"
                     /coded_by="XM_034329689.2:140..1612"
                     /db_xref="GeneID:117606787"
ORIGIN      
        1 mrgikrilvf ctlttivpil flitplylrh nfygnvayav tdsdileitd gistvfcseh
       61 vlqmnrtfna fqmahkpeit sykkhirlkk nmtlpddtle ywgfyllegs svalsvcsrf
      121 egasilvvkg ernlrtcgml ehnneeiven iflpeakqqv kvtfksdtes isnnsiiqvd
      181 hndtlnnttn kffrnitskl eenhtdknat nvhsainsnt lsneeeklrk lvwdtkmymq
      241 qhmkspkepi vhnarklrha kkkqykkqik ekeknmlegr rrtrrdieng skvkekeifk
      301 krtkrnqdlv kpflldqgvk hggnavknlt ngdddssvss fenelfkcyg gsilisqefp
      361 pseqctnvty llngkhmqat hnviengyyy yifysdndiv sndiyalfdi ykpifhyeni
      421 tkscinqtkc sfsinllssd rviveiptkd gleyeiddis lllsvchprm evyvifpiav
      481 lffilacafm
//
LOCUS       XP_034185581             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606787 [Osmia lignaria lignaria].
ACCESSION   XP_034185581
VERSION     XP_034185581.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329690.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC117606787"
                     /calculated_mol_wt=56505
     Region          65..134
                     /region_name="DUF4792"
                     /note="Domain of unknown function (DUF4792); pfam16040"
                     /db_xref="CDD:464988"
     Region          379..487
                     /region_name="DUF4793"
                     /note="Domain of unknown function (DUF4793); pfam16041"
                     /db_xref="CDD:464989"
     CDS             1..490
                     /gene="LOC117606787"
                     /coded_by="XM_034329690.2:149..1621"
                     /db_xref="GeneID:117606787"
ORIGIN      
        1 mrgikrilvf ctlttivpil flitplylrh nfygnvayav tdsdileitd gistvfcseh
       61 vlqmnrtfna fqmahkpeit sykkhirlkk nmtlpddtle ywgfyllegs svalsvcsrf
      121 egasilvvkg ernlrtcgml ehnneeiven iflpeakqqv kvtfksdtes isnnsiiqvd
      181 hndtlnnttn kffrnitskl eenhtdknat nvhsainsnt lsneeeklrk lvwdtkmymq
      241 qhmkspkepi vhnarklrha kkkqykkqik ekeknmlegr rrtrrdieng skvkekeifk
      301 krtkrnqdlv kpflldqgvk hggnavknlt ngdddssvss fenelfkcyg gsilisqefp
      361 pseqctnvty llngkhmqat hnviengyyy yifysdndiv sndiyalfdi ykpifhyeni
      421 tkscinqtkc sfsinllssd rviveiptkd gleyeiddis lllsvchprm evyvifpiav
      481 lffilacafm
//
LOCUS       XP_034185582             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606787 [Osmia lignaria lignaria].
ACCESSION   XP_034185582
VERSION     XP_034185582.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329691.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC117606787"
                     /calculated_mol_wt=56505
     Region          65..134
                     /region_name="DUF4792"
                     /note="Domain of unknown function (DUF4792); pfam16040"
                     /db_xref="CDD:464988"
     Region          379..487
                     /region_name="DUF4793"
                     /note="Domain of unknown function (DUF4793); pfam16041"
                     /db_xref="CDD:464989"
     CDS             1..490
                     /gene="LOC117606787"
                     /coded_by="XM_034329691.2:151..1623"
                     /db_xref="GeneID:117606787"
ORIGIN      
        1 mrgikrilvf ctlttivpil flitplylrh nfygnvayav tdsdileitd gistvfcseh
       61 vlqmnrtfna fqmahkpeit sykkhirlkk nmtlpddtle ywgfyllegs svalsvcsrf
      121 egasilvvkg ernlrtcgml ehnneeiven iflpeakqqv kvtfksdtes isnnsiiqvd
      181 hndtlnnttn kffrnitskl eenhtdknat nvhsainsnt lsneeeklrk lvwdtkmymq
      241 qhmkspkepi vhnarklrha kkkqykkqik ekeknmlegr rrtrrdieng skvkekeifk
      301 krtkrnqdlv kpflldqgvk hggnavknlt ngdddssvss fenelfkcyg gsilisqefp
      361 pseqctnvty llngkhmqat hnviengyyy yifysdndiv sndiyalfdi ykpifhyeni
      421 tkscinqtkc sfsinllssd rviveiptkd gleyeiddis lllsvchprm evyvifpiav
      481 lffilacafm
//
LOCUS       XP_034185583             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606787 [Osmia lignaria lignaria].
ACCESSION   XP_034185583
VERSION     XP_034185583.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329692.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC117606787"
                     /calculated_mol_wt=56505
     Region          65..134
                     /region_name="DUF4792"
                     /note="Domain of unknown function (DUF4792); pfam16040"
                     /db_xref="CDD:464988"
     Region          379..487
                     /region_name="DUF4793"
                     /note="Domain of unknown function (DUF4793); pfam16041"
                     /db_xref="CDD:464989"
     CDS             1..490
                     /gene="LOC117606787"
                     /coded_by="XM_034329692.2:137..1609"
                     /db_xref="GeneID:117606787"
ORIGIN      
        1 mrgikrilvf ctlttivpil flitplylrh nfygnvayav tdsdileitd gistvfcseh
       61 vlqmnrtfna fqmahkpeit sykkhirlkk nmtlpddtle ywgfyllegs svalsvcsrf
      121 egasilvvkg ernlrtcgml ehnneeiven iflpeakqqv kvtfksdtes isnnsiiqvd
      181 hndtlnnttn kffrnitskl eenhtdknat nvhsainsnt lsneeeklrk lvwdtkmymq
      241 qhmkspkepi vhnarklrha kkkqykkqik ekeknmlegr rrtrrdieng skvkekeifk
      301 krtkrnqdlv kpflldqgvk hggnavknlt ngdddssvss fenelfkcyg gsilisqefp
      361 pseqctnvty llngkhmqat hnviengyyy yifysdndiv sndiyalfdi ykpifhyeni
      421 tkscinqtkc sfsinllssd rviveiptkd gleyeiddis lllsvchprm evyvifpiav
      481 lffilacafm
//
LOCUS       XP_034185584             353 aa            linear   INV 03-SEP-2025
DEFINITION  activating signal cointegrator 1 complex subunit 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185584
VERSION     XP_034185584.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329693.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185584.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="activating signal cointegrator 1 complex subunit
                     1"
                     /calculated_mol_wt=40646
     Region          64..129
                     /region_name="KH-I_ASCC1"
                     /note="type I K homology (KH) RNA-binding domain found in
                     activating signal cointegrator 1 complex subunit 1 (ASCC1)
                     and similar proteins; cd22419"
                     /db_xref="CDD:411847"
     Site            order(73,75..77,79..83,86..87,90..91,96..99)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411847"
     Region          139..352
                     /region_name="AKAP7_NLS"
                     /note="AKAP7 2'5' RNA ligase-like domain; pfam10469"
                     /db_xref="CDD:402204"
     CDS             1..353
                     /gene="LOC117606788"
                     /coded_by="XM_034329693.2:203..1264"
                     /db_xref="GeneID:117606788"
ORIGIN      
        1 mnvlkpeltw vdgrcyrlsa ntewsnarnl spyvednchl dyndyedehc dsnieivpyg
       61 gsrfkhtfhv aktffpiiig skntvrkrle tetkttiqip kvgqdgdivi vgsyrkgiit
      121 arhridllme arrkkipith fisiplnegh iimkfnmfks dvlthfekts rgidkmlfqt
      181 psklhltivg ltllddtern qaikalnyce dhivkpmmek hqqiriclqg tdimnddpse
      241 tnvlyakvid pdnvlqeman kilnhfsnig limplndtvk lhvtlmntrf mlddeqreqk
      301 pkrslntfda teimkahkdt flgetilkei hlsqrhtiss ngyyqataki tlh
//
LOCUS       XP_076545853             285 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606789 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545853
VERSION     XP_076545853.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689738.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="uncharacterized protein LOC117606789 isoform X1"
                     /calculated_mol_wt=32830
     CDS             1..285
                     /gene="LOC117606789"
                     /coded_by="XM_076689738.1:2973..3830"
                     /db_xref="GeneID:117606789"
ORIGIN      
        1 mdnvdggesl dnleydtnlv sraenlrrvl gelrnalrie rlnlhqeiem tilqkfierg
       61 nyrslsdgtr rnflmanvar npmkfskfhv tippmrdptr vnnlaylgss myqkiidelq
      121 delnlleqrl saatscsykr kiydleaics adlnrlksrf vrslqpfgnt ihwigqlspr
      181 rikptscrct tkkppmegas lrntsyvrsl ailrsksadt ssdhqswknh iylqrysdse
      241 myrnvykpav slensqilys sdsesnwkms ksittifpkv srvms
//
LOCUS       XP_076545854             278 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606789 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545854
VERSION     XP_076545854.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689739.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="uncharacterized protein LOC117606789 isoform X2"
                     /calculated_mol_wt=32052
     CDS             1..278
                     /gene="LOC117606789"
                     /coded_by="XM_076689739.1:2970..3806"
                     /db_xref="GeneID:117606789"
ORIGIN      
        1 mdnvdggesl dnleydtnlv sraenlrrvl gelrnalrie rlnlhqeiem tilqkfierd
       61 gtrrnflman varnpmkfsk fhvtippmrd ptrvnnlayl gssmyqkiid elqdelnlle
      121 qrlsaatscs ykrkiydlea icsadlnrlk srfvrslqpf gntihwigql sprrikptsc
      181 rcttkkppme gaslrntsyv rslailrsks adtssdhqsw knhiylqrys dsemyrnvyk
      241 pavslensqi lyssdsesnw kmsksittif pkvsrvms
//
LOCUS       XP_034185585             278 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606789 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185585
VERSION     XP_034185585.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329694.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="uncharacterized protein LOC117606789 isoform X3"
                     /calculated_mol_wt=32035
     CDS             1..278
                     /gene="LOC117606789"
                     /coded_by="XM_034329694.2:2971..3807"
                     /db_xref="GeneID:117606789"
ORIGIN      
        1 mdnvdggesl dnleydtnlv sraenlrrvl gelrnalrie rlnlhqeiem tilqkfierg
       61 nyrslsdgtr rnflmanvar npmkfskfhd ptrvnnlayl gssmyqkiid elqdelnlle
      121 qrlsaatscs ykrkiydlea icsadlnrlk srfvrslqpf gntihwigql sprrikptsc
      181 rcttkkppme gaslrntsyv rslailrsks adtssdhqsw knhiylqrys dsemyrnvyk
      241 pavslensqi lyssdsesnw kmsksittif pkvsrvms
//
LOCUS       XP_034185586             271 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606789 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185586
VERSION     XP_034185586.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329695.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..271
                     /product="uncharacterized protein LOC117606789 isoform X4"
                     /calculated_mol_wt=31257
     CDS             1..271
                     /gene="LOC117606789"
                     /coded_by="XM_034329695.2:2966..3781"
                     /db_xref="GeneID:117606789"
ORIGIN      
        1 mdnvdggesl dnleydtnlv sraenlrrvl gelrnalrie rlnlhqeiem tilqkfierd
       61 gtrrnflman varnpmkfsk fhdptrvnnl aylgssmyqk iidelqdeln lleqrlsaat
      121 scsykrkiyd leaicsadln rlksrfvrsl qpfgntihwi gqlsprrikp tscrcttkkp
      181 pmegaslrnt syvrslailr sksadtssdh qswknhiylq rysdsemyrn vykpavslen
      241 sqilyssdse snwkmsksit tifpkvsrvm s
//
LOCUS       XP_034185551             615 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate decarboxylase-like protein black [Osmia lignaria
            lignaria].
ACCESSION   XP_034185551
VERSION     XP_034185551.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185551.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..615
                     /product="glutamate decarboxylase-like protein black"
                     /calculated_mol_wt=69782
     Region          170..614
                     /region_name="GadA"
                     /note="Glutamate or tyrosine decarboxylase or a related
                     PLP-dependent protein [Amino acid transport and
                     metabolism]; COG0076"
                     /db_xref="CDD:439846"
     CDS             1..615
                     /gene="black"
                     /coded_by="XM_034329660.2:121..1968"
                     /db_xref="GeneID:117606773"
ORIGIN      
        1 mpclqnymih rlrrkfpnfi kvllarsvkt rrqerktsrv prnlseqlsr rstsgpdrre
       61 pqcatmpane dtltespeqa vsqirnnflr kvcdsglqnn larsslgded cqngcpnsis
      121 ngdvqedfyy qslpvrqlhe kfmksfvdll leeaifkgts renqvvewme paalqsaidl
      181 klsdqgvsqk dllslasnvi kysvktghpr fvnqlyssvd pygllgqwlt dalnpsvyty
      241 evspvfslme eellremrki vgwkdgrgeg ifcpggsian gyainlarhh rfpqlkelgl
      301 csggklivft sqdahysvkk lsaflgigts nvyevktdsr gkmcvsdlee qirrafdega
      361 tplmvsatag ttvlgaydpl rdlasickky nlwfhvdaaw gggalmsrrh rylldgiela
      421 dsvtwnphkl laapqqcstl llrhegllqa ahglkasylf qpdkfydtsf dsgdkhvqcg
      481 rradvfkfwf mwkakgtrgl ekhvdrvfdl agyfteyirh regfelvlep ectnvcfwyv
      541 ppskrhlqge elskvlqktg ptvkermvkr gsmlityqpl relpnffrlv lqnsgltegd
      601 mrffaeeier landl
//
LOCUS       XP_034185552             542 aa            linear   INV 03-SEP-2025
DEFINITION  alanine aminotransferase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185552
VERSION     XP_034185552.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329661.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185552.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="alanine aminotransferase 1 isoform X1"
                     /calculated_mol_wt=60377
     Region          66..542
                     /region_name="PTZ00377"
                     /note="alanine aminotransferase; Provisional"
                     /db_xref="CDD:240391"
     CDS             1..542
                     /gene="LOC117606774"
                     /coded_by="XM_034329661.2:1..1629"
                     /db_xref="GeneID:117606774"
ORIGIN      
        1 mpqarwwnpv igsgrlrtwe rlsayagsgp tggrtsarlt vtrpvvqrsa vdrtlrrgma
       61 nsapgrkvlt ednvftnlrk meyavrgpll lraleiekel qkgakkpfke vikanvgdah
      121 amgqrpitfl rqvltltvsp nllddptype dvkerartll cgckggsvgs ysesagieiv
      181 rrhvaeyiqn rdglpsdyrn iilsngasdg iksflklfne rldgkpsgvm ipipqyplys
      241 atlaefglaq vgyylneenk wgldiseldr alnesrrtcn prvlvvinpg nptgqvltrs
      301 niediiqfay knhlfllade vyqdnvydkd safhsfkkvm lemgepysqm elasymsvsk
      361 gymgecgirg gyaeiinmdp kvmaillksi samlcptvlg qvvmdvvvnp pkpkepsypl
      421 fikekeetlr slaersqlvv dtlnsipgfk vnpamgamyv fpqidlpkka ieaakakgqh
      481 pdvfyafkll ettgicvipg sgfgqrpgty hfrttilpqk dtikamldsl kqfhlrflee
      541 yk
//
LOCUS       XP_034185553             441 aa            linear   INV 03-SEP-2025
DEFINITION  alanine aminotransferase 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185553
VERSION     XP_034185553.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329662.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="alanine aminotransferase 2 isoform X2"
                     /calculated_mol_wt=49050
     Region          3..441
                     /region_name="PTZ00377"
                     /note="alanine aminotransferase; Provisional"
                     /db_xref="CDD:240391"
     CDS             1..441
                     /gene="LOC117606774"
                     /coded_by="XM_034329662.2:289..1614"
                     /db_xref="GeneID:117606774"
ORIGIN      
        1 mgakkpfkev ikanvgdaha mgqrpitflr qvltltvspn llddptyped vkerartllc
       61 gckggsvgsy sesagieivr rhvaeyiqnr dglpsdyrni ilsngasdgi ksflklfner
      121 ldgkpsgvmi pipqyplysa tlaefglaqv gyylneenkw gldiseldra lnesrrtcnp
      181 rvlvvinpgn ptgqvltrsn iediiqfayk nhlflladev yqdnvydkds afhsfkkvml
      241 emgepysqme lasymsvskg ymgecgirgg yaeiinmdpk vmaillksis amlcptvlgq
      301 vvmdvvvnpp kpkepsyplf ikekeetlrs laersqlvvd tlnsipgfkv npamgamyvf
      361 pqidlpkkai eaakakgqhp dvfyafklle ttgicvipgs gfgqrpgtyh frttilpqkd
      421 tikamldslk qfhlrfleey k
//
LOCUS       XP_034185556             411 aa            linear   INV 03-SEP-2025
DEFINITION  unconventional prefoldin RPB5 interactor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185556
VERSION     XP_034185556.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329665.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185556.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="unconventional prefoldin RPB5 interactor isoform
                     X1"
                     /calculated_mol_wt=47427
     Region          16..137
                     /region_name="Prefoldin_URI1"
                     /note="RNA polymerase II subunit 5-mediating protein
                     homolog; cd23159"
                     /db_xref="CDD:467475"
     Region          16..53
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467475"
     Site            order(42,45..46,49..50,52..53,59..66,68..77,80..84,88..93,
                     102)
                     /site_type="other"
                     /note="putative prefoldin oligomer interface [polypeptide
                     binding]"
                     /db_xref="CDD:467475"
     Region          96..136
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467475"
     Region          150..>210
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     CDS             1..411
                     /gene="uri"
                     /coded_by="XM_034329665.2:310..1545"
                     /db_xref="GeneID:117606775"
ORIGIN      
        1 mltmnyetdv rkfqqalldd allkgiqrne eqckvwrsyk kghekvaetl qtfqkdlhvn
       61 cmvpigkral mkgrlihtne vltflgdgyf akysatgaaa lcqrriqkae emlknlster
      121 dlyetrmmvl egdlfedcag teivehwnee qidewrkkhr ekekeyhqkl skvrqeerkk
      181 ieteedlfnr ldqleieeel adeldrleee gyelfgeelg egeyeyeten sseseqdssr
      241 ekdehilesv keskqnvksg tvksvsfaet dssikkdekl ekaedtksed vegseedilh
      301 iefshsssnp viksdgdsie spadiykifa kpksilkrsp ndvipvqaap pdysteedee
      361 eeeesnkpsa yetvvkdike rdtaapttda sktakdtrpi skfkrdrqlk q
//
LOCUS       XP_076545855             410 aa            linear   INV 03-SEP-2025
DEFINITION  unconventional prefoldin RPB5 interactor isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545855
VERSION     XP_076545855.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689740.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="unconventional prefoldin RPB5 interactor isoform
                     X2"
                     /calculated_mol_wt=47329
     Region          16..136
                     /region_name="Prefoldin_URI1"
                     /note="RNA polymerase II subunit 5-mediating protein
                     homolog; cd23159"
                     /db_xref="CDD:467475"
     Region          16..53
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467475"
     Site            order(42,45..46,49..50,52..53,59..66,68..77,80..84,88..93,
                     102)
                     /site_type="other"
                     /note="putative prefoldin oligomer interface [polypeptide
                     binding]"
                     /db_xref="CDD:467475"
     Region          96..135
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467475"
     Region          149..>209
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     CDS             1..410
                     /gene="uri"
                     /coded_by="XM_076689740.1:313..1545"
                     /db_xref="GeneID:117606775"
ORIGIN      
        1 mltmnyetdv rkfqqalldd allkgiqrne eqckvwrsyk kghekvaetl qtfqkdlhvn
       61 cmvpigkral mkgrlihtne vltflgdgyf akysatgaaa lcqrriqtee mlknlsterd
      121 lyetrmmvle gdlfedcagt eivehwneeq idewrkkhre kekeyhqkls kvrqeerkki
      181 eteedlfnrl dqleieeela deldrleeeg yelfgeelge geyeyetens seseqdssre
      241 kdehilesvk eskqnvksgt vksvsfaetd ssikkdekle kaedtksedv egseedilhi
      301 efshsssnpv iksdgdsies padiykifak pksilkrspn dvipvqaapp dysteedeee
      361 eeesnkpsay etvvkdiker dtaapttdas ktakdtrpis kfkrdrqlkq
//
LOCUS       XP_076545856             350 aa            linear   INV 03-SEP-2025
DEFINITION  unconventional prefoldin RPB5 interactor isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545856
VERSION     XP_076545856.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689741.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="unconventional prefoldin RPB5 interactor isoform
                     X3"
                     /calculated_mol_wt=40173
     Region          <1..76
                     /region_name="Prefoldin_URI1"
                     /note="RNA polymerase II subunit 5-mediating protein
                     homolog; cd23159"
                     /db_xref="CDD:467475"
     Region          35..75
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467475"
     Region          89..>149
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     CDS             1..350
                     /gene="uri"
                     /coded_by="XM_076689741.1:249..1301"
                     /db_xref="GeneID:117606775"
ORIGIN      
        1 mvpigkralm kgrlihtnev ltflgdgyfa kysatgaaal cqrriqkaee mlknlsterd
       61 lyetrmmvle gdlfedcagt eivehwneeq idewrkkhre kekeyhqkls kvrqeerkki
      121 eteedlfnrl dqleieeela deldrleeeg yelfgeelge geyeyetens seseqdssre
      181 kdehilesvk eskqnvksgt vksvsfaetd ssikkdekle kaedtksedv egseedilhi
      241 efshsssnpv iksdgdsies padiykifak pksilkrspn dvipvqaapp dysteedeee
      301 eeesnkpsay etvvkdiker dtaapttdas ktakdtrpis kfkrdrqlkq
//
LOCUS       XP_034185559             243 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606776 [Osmia lignaria lignaria].
ACCESSION   XP_034185559
VERSION     XP_034185559.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329668.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185559.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="uncharacterized protein LOC117606776"
                     /calculated_mol_wt=26887
     Region          79..131
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..243
                     /gene="LOC117606776"
                     /coded_by="XM_034329668.2:129..860"
                     /db_xref="GeneID:117606776"
ORIGIN      
        1 mdrvvrqlvl avmvtigivq cdpplntqyg vpgnyagsnn nnnnnndnnn hgstgtndfg
       61 nhhydnggdn dyvdqpmsye fgyavkdqat gndfgrrets dgetvrgeyr vqlpdgrtqi
      121 vtytadwrtg fhadvryegt atypdqyntq nngynnnnny nnnnqaggqn fgyqgnnnnn
      181 nnapavyngg nnggynyqpp sgnfnsgnny qyqfgsnsld nnynnrnnyn trvpsttygp
      241 afk
//
LOCUS       XP_034185560             217 aa            linear   INV 03-SEP-2025
DEFINITION  C-type lectin 5 [Osmia lignaria lignaria].
ACCESSION   XP_034185560
VERSION     XP_034185560.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329669.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="C-type lectin 5"
                     /calculated_mol_wt=24812
     Region          61..199
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(169,173,178,184,186..189,192..194)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..217
                     /gene="CTL5"
                     /coded_by="XM_034329669.2:152..805"
                     /db_xref="GeneID:117606777"
ORIGIN      
        1 mrtiwlmllf vgvavhaqrr lalpdprsca nrvrhatyrd argvahsyff swehqptrsl
       61 evdwldarni crrhcmdavs letpqenefi kqrlargnvr fiwtsgrkcn fngcdrpdlq
      121 pqningwfws gsgakigptt qrnsgdwsnt ggygqpqpdn reaaqgndes clsilnnfyn
      181 dgikwhdvac hhrkpfvced sdellnfvls rnpgirl
//
LOCUS       XP_076545857             217 aa            linear   INV 03-SEP-2025
DEFINITION  C-type lectin 5 [Osmia lignaria lignaria].
ACCESSION   XP_076545857
VERSION     XP_076545857.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689742.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="C-type lectin 5"
                     /calculated_mol_wt=24812
     Region          61..199
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(169,173,178,184,186..189,192..194)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..217
                     /gene="CTL5"
                     /coded_by="XM_076689742.1:145..798"
                     /db_xref="GeneID:117606777"
ORIGIN      
        1 mrtiwlmllf vgvavhaqrr lalpdprsca nrvrhatyrd argvahsyff swehqptrsl
       61 evdwldarni crrhcmdavs letpqenefi kqrlargnvr fiwtsgrkcn fngcdrpdlq
      121 pqningwfws gsgakigptt qrnsgdwsnt ggygqpqpdn reaaqgndes clsilnnfyn
      181 dgikwhdvac hhrkpfvced sdellnfvls rnpgirl
//
LOCUS       XP_034185561             171 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic iron-sulfur assembly component galla-1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185561
VERSION     XP_034185561.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329670.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="cytosolic iron-sulfur assembly component
                     galla-1"
                     /calculated_mol_wt=19303
     Region          <31..167
                     /region_name="COG5133"
                     /note="Uncharacterized conserved protein [Function
                     unknown]; cl00941"
                     /db_xref="CDD:470001"
     CDS             1..171
                     /gene="galla-1"
                     /coded_by="XM_034329670.2:130..645"
                     /db_xref="GeneID:117606778"
ORIGIN      
        1 mlsffrkisi ggkikdglpt gdemevtegc pdareqtlvs qsdtelkesv ydllrtikdp
       61 ekpqtleqld vvyedcveis rhtpkgisvi riefnptvph cslatliglc irvklerhlv
      121 alfkldiyik qgahsteqei nkqindkeri aaamenpnlr elvekciqee e
//
LOCUS       XP_034185562             134 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606779 [Osmia lignaria lignaria].
ACCESSION   XP_034185562
VERSION     XP_034185562.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329671.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..134
                     /product="uncharacterized protein LOC117606779"
                     /calculated_mol_wt=14029
     Region          47..133
                     /region_name="BRI3"
                     /note="Brain protein I3; pfam10164"
                     /db_xref="CDD:431102"
     CDS             1..134
                     /gene="LOC117606779"
                     /coded_by="XM_034329671.2:838..1242"
                     /db_xref="GeneID:117606779"
ORIGIN      
        1 mekqplnpga akpstisapt apkstsswep ppsynsatrg aestswpppp gyypstgttt
       61 hsnyvpsygs tqstavimpe iilvggcpac rvgvmeddft clgllcailf fpfgiiccll
      121 lktrrcsncg ayfg
//
LOCUS       XP_076545858             134 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606779 [Osmia lignaria lignaria].
ACCESSION   XP_076545858
VERSION     XP_076545858.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689743.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..134
                     /product="uncharacterized protein LOC117606779"
                     /calculated_mol_wt=14029
     Region          47..133
                     /region_name="BRI3"
                     /note="Brain protein I3; pfam10164"
                     /db_xref="CDD:431102"
     CDS             1..134
                     /gene="LOC117606779"
                     /coded_by="XM_076689743.1:766..1170"
                     /db_xref="GeneID:117606779"
ORIGIN      
        1 mekqplnpga akpstisapt apkstsswep ppsynsatrg aestswpppp gyypstgttt
       61 hsnyvpsygs tqstavimpe iilvggcpac rvgvmeddft clgllcailf fpfgiiccll
      121 lktrrcsncg ayfg
//
LOCUS       XP_034185564             123 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L35 [Osmia lignaria lignaria].
ACCESSION   XP_034185564
VERSION     XP_034185564.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329673.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="ribosomal protein L35"
                     /calculated_mol_wt=14339
     Region          8..63
                     /region_name="Ribosomal_L29"
                     /note="Ribosomal L29 protein; pfam00831"
                     /db_xref="CDD:459954"
     CDS             1..123
                     /gene="RpL35"
                     /coded_by="XM_034329673.1:109..480"
                     /db_xref="GeneID:117606780"
ORIGIN      
        1 mgkikctelr tkdkkelikq leelkteltn lrvakvtgga asklskirvv rkaiarvyii
       61 mhqkqkenlr llyknkkykp ldlrpkktra irraltpyqa nrktlkevrk rsafpprkya
      121 ikv
//
LOCUS       XP_034185565             123 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L35 [Osmia lignaria lignaria].
ACCESSION   XP_034185565
VERSION     XP_034185565.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329674.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="ribosomal protein L35"
                     /calculated_mol_wt=14339
     Region          8..63
                     /region_name="Ribosomal_L29"
                     /note="Ribosomal L29 protein; pfam00831"
                     /db_xref="CDD:459954"
     CDS             1..123
                     /gene="RpL35"
                     /coded_by="XM_034329674.2:109..480"
                     /db_xref="GeneID:117606780"
ORIGIN      
        1 mgkikctelr tkdkkelikq leelkteltn lrvakvtgga asklskirvv rkaiarvyii
       61 mhqkqkenlr llyknkkykp ldlrpkktra irraltpyqa nrktlkevrk rsafpprkya
      121 ikv
//
LOCUS       XP_034185566             123 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L35 [Osmia lignaria lignaria].
ACCESSION   XP_034185566
VERSION     XP_034185566.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329675.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="ribosomal protein L35"
                     /calculated_mol_wt=14339
     Region          8..63
                     /region_name="Ribosomal_L29"
                     /note="Ribosomal L29 protein; pfam00831"
                     /db_xref="CDD:459954"
     CDS             1..123
                     /gene="RpL35"
                     /coded_by="XM_034329675.2:109..480"
                     /db_xref="GeneID:117606780"
ORIGIN      
        1 mgkikctelr tkdkkelikq leelkteltn lrvakvtgga asklskirvv rkaiarvyii
       61 mhqkqkenlr llyknkkykp ldlrpkktra irraltpyqa nrktlkevrk rsafpprkya
      121 ikv
//
LOCUS       XP_076545859             123 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L35 [Osmia lignaria lignaria].
ACCESSION   XP_076545859
VERSION     XP_076545859.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689744.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="ribosomal protein L35"
                     /calculated_mol_wt=14339
     Region          8..63
                     /region_name="Ribosomal_L29"
                     /note="Ribosomal L29 protein; pfam00831"
                     /db_xref="CDD:459954"
     CDS             1..123
                     /gene="RpL35"
                     /coded_by="XM_076689744.1:109..480"
                     /db_xref="GeneID:117606780"
ORIGIN      
        1 mgkikctelr tkdkkelikq leelkteltn lrvakvtgga asklskirvv rkaiarvyii
       61 mhqkqkenlr llyknkkykp ldlrpkktra irraltpyqa nrktlkevrk rsafpprkya
      121 ikv
//
LOCUS       XP_076545860             125 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305590 [Osmia lignaria lignaria].
ACCESSION   XP_076545860
VERSION     XP_076545860.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689745.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="uncharacterized protein LOC143305590"
                     /calculated_mol_wt=14122
     CDS             1..125
                     /gene="LOC143305590"
                     /coded_by="XM_076689745.1:55..432"
                     /db_xref="GeneID:143305590"
ORIGIN      
        1 mrfsrkresc rndgmsrlsa revsqarlll rgragekspv vrerqktqip vpllqilpgs
       61 lvssigdwri lddlrrfpgq grlqvqqfly iwkignavne apekisetlp lqgvsredqg
      121 vsenr
//
LOCUS       XP_034190589            1050 aa            linear   INV 03-SEP-2025
DEFINITION  coiled coil domain containing protein Alhambra isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190589
VERSION     XP_034190589.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334698.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1050
                     /product="coiled coil domain containing protein Alhambra
                     isoform X1"
                     /calculated_mol_wt=110733
     Region          7..54
                     /region_name="PHD_AF10_AF17"
                     /note="PHD finger found in protein AF-10 and AF-17;
                     cd15574"
                     /db_xref="CDD:277049"
     Site            order(7,21..25,31,49)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277049"
     Region          65..180
                     /region_name="ePHD_AF10_like"
                     /note="Extended PHD finger found in protein AF-10 and
                     AF-17; cd15672"
                     /db_xref="CDD:277142"
     Site            order(119,136..140,146,175)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277142"
     Region          794..857
                     /region_name="CC_AF10"
                     /note="coiled coil domain of ALL1-Fused gene from
                     chromosome 10 protein (AF10) and similar proteins;
                     cd20901"
                     /db_xref="CDD:411015"
     Site            order(822,825..826,829..830,832..833,836..837,840,
                     843..844,846..847,850)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411015"
     Region          <860..>995
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     CDS             1..1050
                     /gene="Alh"
                     /coded_by="XM_034334698.2:538..3690"
                     /db_xref="GeneID:117608932"
ORIGIN      
        1 mkemlggccv csdergwten plvycdgqgc tvavhqacyg ivtvptgpwy crkcesqers
       61 arvrcelcps rdgalkrtdq agwahvvcal yipevrfgnv ttmepiilql ipserfsksc
      121 yiceeqgrgs ranvgacmqc nktgcrqqfh vtcaqalgll ceeagnyldn vkycgycqhh
      181 ysklkkggnv ktippykpip adngssdssp ekeaetpiks sssgkrksss sksttnsknk
      241 sntspslein gvaddksssg rntpkdnttn sskfttsnfv etivtqsesv fghdgttstt
      301 avaaatiksg snsnsghksg gqtksnssss nktsshskkr ktgartptvi gnensnqsvs
      361 seasgsvvva vssivqqavs snniqttite atslstttep ekskklkves piqssntpvt
      421 teatttpvim tvtqsqssti aqsptttsns ivvsvpltat slsntvqsvv tsaptlyqql
      481 qqsiittaan mearanavsn serfvneeap akrsrsqssd kqdksrkpkr gssnsqpala
      541 qaqvpsqphq qqqqqqsqps assvvtsvss savvttskrg grsnhqtppp aitvapipsi
      601 iqgptlgtgh fssvnsgstn lmgptvkesp psspgsesms sngpgrrkrk sasaasttpt
      661 psasstptlt hpkeekdikl fqngvsaphm lgnqlnptst maqkmsdtls qeleahsift
      721 easnsttnlv gpqlhsrvia sirsnqtgap ptsfssvfnt nsntnsnmni nsnasasvni
      781 agtgalgggt ipqtldqlle rqweqgsqfl meqaqhfdia sllsclhqlr aenlrleehv
      841 nsllqrrdhl lavnarlaip lttqpsahpv nnihpnvnps hpnvphpevp pgaaapvprv
      901 nreprvnnty mphssasnhs aglenglppd psppaaasls qyqhrtslva pqpsptirhs
      961 pagsgyhqgq psnivspapt gnsgvvrnss vtpdpvrgvp rsvpqsssny mpsmyqptvs
     1021 sltsnqvgnv hnlhshgpsp pshgppgkps
//
LOCUS       XP_034190590            1007 aa            linear   INV 03-SEP-2025
DEFINITION  coiled coil domain containing protein Alhambra isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190590
VERSION     XP_034190590.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334699.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1007
                     /product="coiled coil domain containing protein Alhambra
                     isoform X2"
                     /calculated_mol_wt=106429
     Region          7..54
                     /region_name="PHD_AF10_AF17"
                     /note="PHD finger found in protein AF-10 and AF-17;
                     cd15574"
                     /db_xref="CDD:277049"
     Site            order(7,21..25,31,49)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277049"
     Region          65..180
                     /region_name="ePHD_AF10_like"
                     /note="Extended PHD finger found in protein AF-10 and
                     AF-17; cd15672"
                     /db_xref="CDD:277142"
     Site            order(119,136..140,146,175)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277142"
     Region          794..857
                     /region_name="CC_AF10"
                     /note="coiled coil domain of ALL1-Fused gene from
                     chromosome 10 protein (AF10) and similar proteins;
                     cd20901"
                     /db_xref="CDD:411015"
     Site            order(822,825..826,829..830,832..833,836..837,840,
                     843..844,846..847,850)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411015"
     Region          855..>926
                     /region_name="PHA01929"
                     /note="putative scaffolding protein"
                     /db_xref="CDD:177328"
     CDS             1..1007
                     /gene="Alh"
                     /coded_by="XM_034334699.2:538..3561"
                     /db_xref="GeneID:117608932"
ORIGIN      
        1 mkemlggccv csdergwten plvycdgqgc tvavhqacyg ivtvptgpwy crkcesqers
       61 arvrcelcps rdgalkrtdq agwahvvcal yipevrfgnv ttmepiilql ipserfsksc
      121 yiceeqgrgs ranvgacmqc nktgcrqqfh vtcaqalgll ceeagnyldn vkycgycqhh
      181 ysklkkggnv ktippykpip adngssdssp ekeaetpiks sssgkrksss sksttnsknk
      241 sntspslein gvaddksssg rntpkdnttn sskfttsnfv etivtqsesv fghdgttstt
      301 avaaatiksg snsnsghksg gqtksnssss nktsshskkr ktgartptvi gnensnqsvs
      361 seasgsvvva vssivqqavs snniqttite atslstttep ekskklkves piqssntpvt
      421 teatttpvim tvtqsqssti aqsptttsns ivvsvpltat slsntvqsvv tsaptlyqql
      481 qqsiittaan mearanavsn serfvneeap akrsrsqssd kqdksrkpkr gssnsqpala
      541 qaqvpsqphq qqqqqqsqps assvvtsvss savvttskrg grsnhqtppp aitvapipsi
      601 iqgptlgtgh fssvnsgstn lmgptvkesp psspgsesms sngpgrrkrk sasaasttpt
      661 psasstptlt hpkeekdikl fqngvsaphm lgnqlnptst maqkmsdtls qeleahsift
      721 easnsttnlv gpqlhsrvia sirsnqtgap ptsfssvfnt nsntnsnmni nsnasasvni
      781 agtgalgggt ipqtldqlle rqweqgsqfl meqaqhfdia sllsclhqlr aenlrleehv
      841 nsllqrrdhl lavnarlaip lttqpsahpv nnihpnvnps hpnvphpevp pgaaapvprv
      901 nreprvnnty mphssasnhs aglenglppd psppaaasls qyqhrtslva pqpsptirsv
      961 pqsssnymps myqptvsslt snqvgnvhnl hshgpsppsh gppgkps
//
LOCUS       XP_034190591             993 aa            linear   INV 03-SEP-2025
DEFINITION  coiled coil domain containing protein Alhambra isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190591
VERSION     XP_034190591.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334700.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..993
                     /product="coiled coil domain containing protein Alhambra
                     isoform X3"
                     /calculated_mol_wt=104738
     Region          7..54
                     /region_name="PHD_AF10_AF17"
                     /note="PHD finger found in protein AF-10 and AF-17;
                     cd15574"
                     /db_xref="CDD:277049"
     Site            order(7,21..25,31,49)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277049"
     Region          65..180
                     /region_name="ePHD_AF10_like"
                     /note="Extended PHD finger found in protein AF-10 and
                     AF-17; cd15672"
                     /db_xref="CDD:277142"
     Site            order(119,136..140,146,175)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277142"
     Region          737..800
                     /region_name="CC_AF10"
                     /note="coiled coil domain of ALL1-Fused gene from
                     chromosome 10 protein (AF10) and similar proteins;
                     cd20901"
                     /db_xref="CDD:411015"
     Site            order(765,768..769,772..773,775..776,779..780,783,
                     786..787,789..790,793)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411015"
     Region          <803..>938
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     CDS             1..993
                     /gene="Alh"
                     /coded_by="XM_034334700.2:538..3519"
                     /db_xref="GeneID:117608932"
ORIGIN      
        1 mkemlggccv csdergwten plvycdgqgc tvavhqacyg ivtvptgpwy crkcesqers
       61 arvrcelcps rdgalkrtdq agwahvvcal yipevrfgnv ttmepiilql ipserfsksc
      121 yiceeqgrgs ranvgacmqc nktgcrqqfh vtcaqalgll ceeagnyldn vkycgycqhh
      181 ysklkkggnv ktippykpip adngssdssp ekeaetpiks sssetivtqs esvfghdgtt
      241 sttavaaati ksgsnsnsgh ksggqtksns sssnktsshs kkrktgartp tvignensnq
      301 svsseasgsv vvavssivqq avssnniqtt iteatslstt tepekskklk vespiqssnt
      361 pvtteatttp vimtvtqsqs stiaqspttt snsivvsvpl tatslsntvq svvtsaptly
      421 qqlqqsiitt aanmearana vsnserfvne eapakrsrsq ssdkqdksrk pkrgssnsqp
      481 alaqaqvpsq phqqqqqqqs qpsassvvts vsssavvtts krggrsnhqt pppaitvapi
      541 psiiqgptlg tghfssvnsg stnlmgptvk esppsspgse smssngpgrr krksasaast
      601 tptpsasstp tlthpkeekd iklfqngvsa phmlgnqlnp tstmaqkmsd tlsqeleahs
      661 ifteasnstt nlvgpqlhsr viasirsnqt gapptsfssv fntnsntnsn mninsnasas
      721 vniagtgalg ggtipqtldq llerqweqgs qflmeqaqhf diasllsclh qlraenlrle
      781 ehvnsllqrr dhllavnarl aiplttqpsa hpvnnihpnv npshpnvphp evppgaaapv
      841 prvnreprvn ntymphssas nhsaglengl ppdpsppaaa slsqyqhrts lvapqpspti
      901 rhspagsgyh qgqpsnivsp aptgnsgvvr nssvtpdpvr gvprsvpqss snympsmyqp
      961 tvssltsnqv gnvhnlhshg psppshgppg kps
//
LOCUS       XP_076545861             948 aa            linear   INV 03-SEP-2025
DEFINITION  coiled coil domain containing protein Alhambra isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545861
VERSION     XP_076545861.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689746.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..948
                     /product="coiled coil domain containing protein Alhambra
                     isoform X4"
                     /calculated_mol_wt=99472
     Region          <1..78
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(17,35..38,44,73)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:277142"
     Region          692..755
                     /region_name="CC_AF10"
                     /note="coiled coil domain of ALL1-Fused gene from
                     chromosome 10 protein (AF10) and similar proteins;
                     cd20901"
                     /db_xref="CDD:411015"
     Site            order(720,723..724,727..728,730..731,734..735,738,
                     741..742,744..745,748)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411015"
     Region          <758..>893
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     CDS             1..948
                     /gene="Alh"
                     /coded_by="XM_076689746.1:215..3061"
                     /db_xref="GeneID:117608932"
ORIGIN      
        1 mepiilqlip serfskscyi ceeqgrgsra nvgacmqcnk tgcrqqfhvt caqalgllce
       61 eagnyldnvk ycgycqhhys klkkggnvkt ippykpipad ngssdsspek eaetpiksss
      121 sgkrkssssk sttnsknksn tspsleingv addksssgrn tpkdnttnss kfttsnfvet
      181 ivtqsesvfg hdgttsttav aaatiksgsn snsghksggq tksnssssnk tsshskkrkt
      241 gartptvign ensnqsvsse asgsvvvavs sivqqavssn niqttiteat slstttepek
      301 skklkvespi qssntpvtte atttpvimtv tqsqsstiaq sptttsnsiv vsvpltatsl
      361 sntvqsvvts aptlyqqlqq siittaanme aranavsnse rfvneeapak rsrsqssdkq
      421 dksrkpkrgs snsqpalaqa qvpsqphqqq qqqqsqpsas svvtsvsssa vvttskrggr
      481 snhqtpppai tvapipsiiq gptlgtghfs svnsgstnlm gptvkespps spgsesmssn
      541 gpgrrkrksa saasttptps asstptlthp keekdiklfq ngvsaphmlg nqlnptstma
      601 qkmsdtlsqe leahsiftea snsttnlvgp qlhsrviasi rsnqtgappt sfssvfntns
      661 ntnsnmnins nasasvniag tgalgggtip qtldqllerq weqgsqflme qaqhfdiasl
      721 lsclhqlrae nlrleehvns llqrrdhlla vnarlaiplt tqpsahpvnn ihpnvnpshp
      781 nvphpevppg aaapvprvnr eprvnntymp hssasnhsag lenglppdps ppaaaslsqy
      841 qhrtslvapq psptirhspa gsgyhqgqps nivspaptgn sgvvrnssvt pdpvrgvprs
      901 vpqsssnymp smyqptvssl tsnqvgnvhn lhshgpspps hgppgkps
//
LOCUS       XP_034190596             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608934 [Osmia lignaria lignaria].
ACCESSION   XP_034190596
VERSION     XP_034190596.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334705.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190596.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC117608934"
                     /calculated_mol_wt=54435
     CDS             1..486
                     /gene="LOC117608934"
                     /coded_by="XM_034334705.2:296..1756"
                     /db_xref="GeneID:117608934"
ORIGIN      
        1 mvddeeqeel rctgsdveie eytdteespy vtyqatdklf dtdragwqkf sfiatllivv
       61 vsvtfliiay plylnsistv snaytgllft alsstcllgi iwfiiervsp ppalipnsmk
      121 ikiprcalvk isliyafsgi vttlaldqnr vlchlqdpik gitlvfslvy yfffcrkmms
      181 lqrifsstti ivglfisvdy glcdefrcrg revsphaasi reswgaravw tfvyvgalaa
      241 famfftlleg hytteqqnmc hvmanqqnsf lytvsrlvss rdirrrgsee eggrllhvtd
      301 pdptvkpkhf pkppiletlf yihliaffai ltmswvntlp gigrgyspve lyrtvehglt
      361 chfkntescs nvsihgwtfl iaytvfsisv lnflsmcesa vftvaaatvs lplsgiwwsi
      421 ykmdisvhgg siswspgvtg elicallglp vvllglgllv rshfrdtqps yltmqppevq
      481 cehsqr
//
LOCUS       XP_034190597             260 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608935 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190597
VERSION     XP_034190597.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334706.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190597.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="uncharacterized protein LOC117608935 isoform X1"
                     /calculated_mol_wt=28898
     CDS             1..260
                     /gene="LOC117608935"
                     /coded_by="XM_034334706.2:155..937"
                     /db_xref="GeneID:117608935"
ORIGIN      
        1 mrtrnrmppy rvwgsiedgs iefesltddt legainvire sffvyesvcr gvdllsepga
       61 skeleklcld aakdgisvva idvnsgevig vafnkmqvaq nsaeksafei fsenckyqss
      121 kalvdfmidv dsrinlfkhy ntdcifeimf latltskqkr rigellvsss melakelkke
      181 kpvktpvtid gdntlqnkda ipslisaimt ssysqkiask cgfedlikvp yekfsfkgks
      241 fsdrigdehp scvlvakrlq
//
LOCUS       XP_076545862             260 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608935 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545862
VERSION     XP_076545862.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689747.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="uncharacterized protein LOC117608935 isoform X1"
                     /calculated_mol_wt=28898
     CDS             1..260
                     /gene="LOC117608935"
                     /coded_by="XM_076689747.1:155..937"
                     /db_xref="GeneID:117608935"
ORIGIN      
        1 mrtrnrmppy rvwgsiedgs iefesltddt legainvire sffvyesvcr gvdllsepga
       61 skeleklcld aakdgisvva idvnsgevig vafnkmqvaq nsaeksafei fsenckyqss
      121 kalvdfmidv dsrinlfkhy ntdcifeimf latltskqkr rigellvsss melakelkke
      181 kpvktpvtid gdntlqnkda ipslisaimt ssysqkiask cgfedlikvp yekfsfkgks
      241 fsdrigdehp scvlvakrlq
//
LOCUS       XP_034190598             254 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608935 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190598
VERSION     XP_034190598.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334707.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190598.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="uncharacterized protein LOC117608935 isoform X2"
                     /calculated_mol_wt=28083
     CDS             1..254
                     /gene="LOC117608935"
                     /coded_by="XM_034334707.2:154..918"
                     /db_xref="GeneID:117608935"
ORIGIN      
        1 mppyrvwgsi edgsiefesl tddtlegain viresffvye svcrgvdlls epgaskelek
       61 lcldaakdgi svvaidvnsg evigvafnkm qvaqnsaeks afeifsenck yqsskalvdf
      121 midvdsrinl fkhyntdcif eimflatlts kqkrrigell vsssmelake lkkekpvktp
      181 vtidgdntlq nkdaipslis aimtssysqk iaskcgfedl ikvpyekfsf kgksfsdrig
      241 dehpscvlva krlq
//
LOCUS       XP_034190600             243 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608937 [Osmia lignaria lignaria].
ACCESSION   XP_034190600
VERSION     XP_034190600.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334709.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="uncharacterized protein LOC117608937"
                     /calculated_mol_wt=27402
     Region          <124..>163
                     /region_name="Acetyltransf_1"
                     /note="Acetyltransferase (GNAT) family; pfam00583"
                     /db_xref="CDD:395465"
     Region          <130..214
                     /region_name="RimL"
                     /note="Protein N-acetyltransferase, RimJ/RimL family
                     [Translation, ribosomal structure and biogenesis,
                     Posttranslational modification, protein turnover,
                     chaperones]; COG1670"
                     /db_xref="CDD:441276"
     Site            order(139..141,151..152)
                     /site_type="other"
                     /note="Coenzyme A binding pocket [chemical binding]"
                     /db_xref="CDD:173926"
     CDS             1..243
                     /gene="LOC117608937"
                     /coded_by="XM_034334709.2:169..900"
                     /db_xref="GeneID:117608937"
ORIGIN      
        1 mslssneqle fklltrdkvq ealdiqtetm kqeclaigfg mfeevgaaee mrmvfteivk
       61 dgstimavdr etdqlaavaf nkiharpreg ekdqleifmk enlkhracqe lvnfldsiqm
      121 nvdifknyna ngaielfylg tdpryqgrgi gsqmvqkcie fgrgllngtm krssidgsig
      181 neqvlpefiy gvfasnysqr iaetigfevl hevryddysf agkklsemig dvhrsarlqg
      241 lkl
//
LOCUS       XP_034190599             236 aa            linear   INV 03-SEP-2025
DEFINITION  arylalkylamine N-acetyltransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190599
VERSION     XP_034190599.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334708.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190599.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="arylalkylamine N-acetyltransferase 1"
                     /calculated_mol_wt=26380
     Region          103..>173
                     /region_name="MnaT"
                     /note="L-amino acid N-acyltransferase MnaT [Amino acid
                     transport and metabolism]; COG1247"
                     /db_xref="CDD:440860"
     Region          143..>173
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     Region          <148..212
                     /region_name="Acetyltransf_10"
                     /note="Acetyltransferase (GNAT) domain; pfam13673"
                     /db_xref="CDD:463953"
     CDS             1..236
                     /gene="LOC117608936"
                     /coded_by="XM_034334708.2:409..1119"
                     /db_xref="GeneID:117608936"
ORIGIN      
        1 melsndlcnf kwatsshlpe ilsflyenfd keetmltslr dnntltaede ksmrmdherl
       61 iraifafspc liavekslkk iigvnlmivs rnskfddkad gvsaafannp pttklmkqyf
      121 nylsviseka dlfdkfpnar vavefyavai dknyrrrgls kalmaagisf akdtvqdagf
      181 ifgvytslys kkaaetlglk svmdvdllty kdadgqpifq dtpphnivsv mvlqla
//
LOCUS       XP_034190682            1735 aa            linear   INV 03-SEP-2025
DEFINITION  intraflagellar transport protein Oseg2 [Osmia lignaria lignaria].
ACCESSION   XP_034190682
VERSION     XP_034190682.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334791.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1735
                     /product="intraflagellar transport protein Oseg2"
                     /calculated_mol_wt=193063
     Region          <16..304
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          19..60
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          70..108
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          115..185
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          201..233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          242..281
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <454..568
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          <862..1414
                     /region_name="COG3899"
                     /note="Predicted ATPase [General function prediction
                     only]"
                     /db_xref="CDD:443106"
     CDS             1..1735
                     /gene="Oseg2"
                     /coded_by="XM_034334791.2:102..5309"
                     /db_xref="GeneID:117608978"
ORIGIN      
        1 mllkylgnvm spyeyenrvv sivwspnnlk lavassdrci ylfddkcvkr dkfstkpvds
       61 kfgkksyvik aiafspdstk iavgqtdcmi yvykigeqwg ekkvicnkfr qsssvtcliw
      121 ltegpiivgl vdgkvraalv ksqkaqtlyt adsttialas nirgtgflss hadgniikyh
      181 itddgsheps gricthtvpa yalawpqshi laagcdrrvt fydsrgklvk nfdysrenek
      241 eiavaccsps gqsvaigswd kirildwspr rsiweeantk slpnfytvta iswrrdgsrl
      301 lvgslcgave qfetvlkrtv irgshevayv gpsqvvirpl negnrpviir sqtgneiedv
      361 kvlgradnnv vartantlll adielnlise ipwddksnge kfffeyprvc lifcsgelti
      421 veygnneplg svrteavnph vvsvrinerq ipgapdnkrl aylldprtvr itdlitgitv
      481 amishdvrvd wlelsetghr llsrdkrarl wlsdelggri llltgvsfvs wvlgsdvvva
      541 qtgqtlavwy nvdapevatl ipvrgdavdi vredgrssvm veevggkvay lldepliefg
      601 talhdndfgr allfleelad rpqaeamwen varnamatrq llvaarcyaa lgdvacsrfl
      661 knvvqigeky seetgndpls spdcwaklai lngelktaea iyleqneldk aldmyqsywh
      721 wedalmlaqn rgylglqelr drhltwlles gqaaraaail entnprravk lyldarrpgr
      781 aarlvladde llndksivne ivrvlkatdl melagellek tsepqeaikc ysqagvfara
      841 velarevdpt svvdlerdwg khlasaghyd aainhfieag etalaldaai narqwrkglq
      901 ivqviehdnp tirkqceklg eyfasigeks laeklfirag darravevhv qsgnwsrahe
      961 vaqaymtpge anevlskhav dlceagdlkh aeelylaigq ydsaismykk agrrpdmirl
     1021 vakyrsdlld athvhlakel dasgkpreae ehylaagdwk aaiaayrsan mwedalrvak
     1081 qnagdnaaqq vallwartla pelaarllir lnyldsclql aceaglfdwa leiskygsae
     1141 qkkevhyrya maledagrfs eaekefikae rmmeavqmyi hnrdweaaed vaqsinqdvv
     1201 aqvliarane aaeaqdysla etlllraykp emiiehykka gmwsealrvc reylpsqean
     1261 lrrelgqksa slaganalee akkwlevgev raaldilvld pqaprpslir aadillhqad
     1321 petaaqvggd lgarlfavge halaaqvflq adrlkdaida lasvgewera kriarelapd
     1381 iepyleekyk eamlrdgqid rlveidaeag levlankgqw nqvfetassq gsqvlhkyva
     1441 qravqlmksn aslealqlyv rygtppvpqn fnlylqlses vlnseayhey kylallrtvl
     1501 ldlwnslkss edaprlkfer lleathysav kqgcrdysvl sglvlkasit llrytdiila
     1561 drayyeagte artaglnsea fvflnhfldl eecieegdst vmdvddlavt dfpvevplpe
     1621 slslsaqqre earewvlavs mdqkveqvlp adhrgvyvgs ltspssgaga lqgciltgyp
     1681 vrgpiirfae gkraadrddw tkmintarqa pqdsvlndil afiqewcgav pnysf
//
LOCUS       XP_034190684            1694 aa            linear   INV 03-SEP-2025
DEFINITION  ADP ribosylation factor guanine nucleotide exchange factor Sec71
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190684
VERSION     XP_034190684.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334793.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1694
                     /product="ADP ribosylation factor guanine nucleotide
                     exchange factor Sec71 isoform X1"
                     /calculated_mol_wt=191292
     Region          10..1627
                     /region_name="PLN03076"
                     /note="ARF guanine nucleotide exchange factor (ARF-GEF);
                     Provisional"
                     /db_xref="CDD:215560"
     CDS             1..1694
                     /gene="Sec71"
                     /coded_by="XM_034334793.2:407..5491"
                     /db_xref="GeneID:117608979"
ORIGIN      
        1 mqdnskemfi iralekilad rdvkrshlsq lrkscetald nlrneikegt nvqvstalpq
       61 prsdsyvisa ekyflpfela cqsksprivv taldclqkli ayghltgnvp dstepnklli
      121 vriveticgc fmgpqtdegv qlqiikallt vmtsqhvevh egtvlltirt vysvylasrn
      181 lvnqttarat ltqminvifa rmetqaeeen vrsevehsat tvntctsgel etetvnheeh
      241 stesnqepql ivrgiledvv nsvvpedpai avtvtseeas ldqvpidens deavaesdnm
      301 vrakfthvlq kdaflvfral cklsmkplpd gtpdpkshql rskilslqll lgilqnagpv
      361 lrsnemfvia ikqylcvals kngvssvpev felslalfla llarfkmhlk mqievffkei
      421 fmniletsss sfehkwmvih altricadaq svvdiyvnyd cdlsaanlfe rlvndlskia
      481 qgrqalelga spnqeksmri rgleclvsil kcmvewsrdl yvnpsvpadq qipseppdpp
      541 vepplprygs agslssanss lvgnkeipds peqyevqkqq kevwetgidi fnrkpskgvq
      601 ylqeqgllgn ssedvarwlh mderldktai gdflgdhnhn qvmysyidqm nfadrdlvta
      661 lryflegfrl pgeaqkidrl mekfasryce cnpnnglfts adtayvlgfs iimlttdlhs
      721 pqvknkmtke qyiklnrris dnedlpeeyl skiydeiagn eikmksnpnr pgkqvissek
      781 krrllwnmem evistaaknl mesvshvqap fttakhlehv rpmfkiawtp flaafsvglq
      841 dcddpeiasl cldgircair iacifhmtle rdayvqalar ftlltanspi temkaknidt
      901 iktlitvaht dgnylgsswl dvvkcisqle laqligtgvr pqllgppskp hfpsplvnfn
      961 lahnnshqnn slnlssldps vkesigetss qsvvvavdri ftgstrldgd aivefvkalc
     1021 qvsleelshp tqprmfsltk iveisyynmg rirlqwsriw qvigdhfdrv gcsprqdiaf
     1081 favdslrqla tkfiekgefa nfrfqkdflr pfehimkknr spvirdmvvr cvaqivhsqa
     1141 pnirsgwkni fsvfhhaasd rdeavvelaf smtgkiinel yaedfsimvd sfqdavkcls
     1201 efacnasfpe tsmeairlir scasyidanp nlfaegmmdd sgmvseedra wvrgwfpllf
     1261 elscivsrck ldvrtraltv lfdvvkthga sfkphwwkdl fqvlfrifdn mklpeqhtek
     1321 aewmtttcnh alyaivdvfs qfydtlgpll leqlysqllw cvqqdneqla rsgtnclenl
     1381 visngikfde qtwektcscv ldifestlps allswkplsp nkesdldvit getdnhvgil
     1441 krsnssqslt nsesgknklf yallikcvvq leliqtidni vfypatsrke dqenlalaqa
     1501 dmfngkssel gvragadqqk eeqgmycalt tmhllqlvec llkshrfaks fnsnheqrnv
     1561 lwkagfrgnv kpnllkqetq slacalrilf kmysdeahra dwskvesrlv evacealeyf
     1621 lalsneahrd awtpilllll trilkmsdnr favhasscyp llcevmcfdl kpelrsvlrr
     1681 fflrigpvfr itqq
//
LOCUS       XP_034190685            1667 aa            linear   INV 03-SEP-2025
DEFINITION  ADP ribosylation factor guanine nucleotide exchange factor Sec71
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190685
VERSION     XP_034190685.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334794.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1667
                     /product="ADP ribosylation factor guanine nucleotide
                     exchange factor Sec71 isoform X2"
                     /calculated_mol_wt=188094
     Region          10..1600
                     /region_name="PLN03076"
                     /note="ARF guanine nucleotide exchange factor (ARF-GEF);
                     Provisional"
                     /db_xref="CDD:215560"
     CDS             1..1667
                     /gene="Sec71"
                     /coded_by="XM_034334794.2:407..5410"
                     /db_xref="GeneID:117608979"
ORIGIN      
        1 mqdnskemfi iralekilad rdvkrshlsq lrkscetald nlrneikegt nvqvstalpq
       61 prsdsyvisa ekyflpfela cqsksprivv taldclqkli ayghltgnvp dstepnklli
      121 vriveticgc fmgpqtdegv qlqiikallt vmtsqhvevh egtvlltirt vysvylasrn
      181 lvnqttarat ltqminvifa rmetqaeeen vrsevehsat tvntctsgel etetvnheeh
      241 stesnqepql ivrgiledvv nsvvpedpai avtvtseeas ldqvpidens deavaesdnm
      301 vrakfthvlq kdaflvfral cklsmkplpd gtpdpkshql rskilslqll lgilqnagpv
      361 lrsnemfvia ikqylcvals kngvssvpev felslalfla llarfkmhlk mqievffkei
      421 fmniletsss sfehkwmvih altricadaq svvdiyvnyd cdlsaanlfe rlvndlskia
      481 qgrqalelga spnqeksmri rgleclvsil kcmvewsrdl yvnpsvpadq qipseppdpp
      541 vepplprygs agslssanss lvgnkeipds peqyevqkqq kevwetgidi fnrkpskgvq
      601 ylqeqgllgn ssedvarwlh mderldktai vtalryfleg frlpgeaqki drlmekfasr
      661 ycecnpnngl ftsadtayvl gfsiimlttd lhspqvknkm tkeqyiklnr risdnedlpe
      721 eylskiydei agneikmksn pnrpgkqvis sekkrrllwn memevistaa knlmesvshv
      781 qapfttakhl ehvrpmfkia wtpflaafsv glqdcddpei aslcldgirc airiacifhm
      841 tlerdayvqa larftlltan spitemkakn idtiktlitv ahtdgnylgs swldvvkcis
      901 qlelaqligt gvrpqllgpp skphfpsplv nfnlahnnsh qnnslnlssl dpsvkesige
      961 tssqsvvvav driftgstrl dgdaivefvk alcqvsleel shptqprmfs ltkiveisyy
     1021 nmgrirlqws riwqvigdhf drvgcsprqd iaffavdslr qlatkfiekg efanfrfqkd
     1081 flrpfehimk knrspvirdm vvrcvaqivh sqapnirsgw knifsvfhha asdrdeavve
     1141 lafsmtgkii nelyaedfsi mvdsfqdavk clsefacnas fpetsmeair lirscasyid
     1201 anpnlfaegm mddsgmvsee drawvrgwfp llfelscivs rckldvrtra ltvlfdvvkt
     1261 hgasfkphww kdlfqvlfri fdnmklpeqh tekaewmttt cnhalyaivd vfsqfydtlg
     1321 pllleqlysq llwcvqqdne qlarsgtncl enlvisngik fdeqtwektc scvldifest
     1381 lpsallswkp lspnkesdld vitgetdnhv gilkrsnssq sltnsesgkn klfyallikc
     1441 vvqleliqti dnivfypats rkedqenlal aqadmfngks selgvragad qqkeeqgmyc
     1501 alttmhllql vecllkshrf aksfnsnheq rnvlwkagfr gnvkpnllkq etqslacalr
     1561 ilfkmysdea hradwskves rlvevaceal eyflalsnea hrdawtpill llltrilkms
     1621 dnrfavhass cypllcevmc fdlkpelrsv lrrfflrigp vfritqq
//
LOCUS       XP_034190255             422 aa            linear   INV 03-SEP-2025
DEFINITION  protein CLP1 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034190255
VERSION     XP_034190255.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334364.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190255.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="protein CLP1 homolog"
                     /calculated_mol_wt=46513
     Region          10..420
                     /region_name="CLP1"
                     /note="Predicted GTPase subunit of the pre-mRNA cleavage
                     complex [Translation, ribosomal structure and biogenesis];
                     COG5623"
                     /db_xref="CDD:227910"
     Region          118..303
                     /region_name="CLP1_P"
                     /note="mRNA cleavage and polyadenylation factor CLP1
                     P-loop; pfam16575"
                     /db_xref="CDD:406878"
     CDS             1..422
                     /gene="cbc"
                     /coded_by="XM_034334364.2:193..1461"
                     /db_xref="GeneID:117608775"
ORIGIN      
        1 mtdekaqtqe fkldpdcelr fevesknekv tlelktglae vfgtelvkgk kyeftagakv
       61 avftwqgctv elvgktdvsy vaketpmgly lnchaamerl renaekddtr gpitmvvgpc
      121 dvgkstlcrl llnyavrmgr rpifvdldvg qghiaipgtv gallverpss ivegfsqqap
      181 lvfhfghksp nanvalynll vtrlaevcsd rlqankkarv sgivintcgw vkgdgykllt
      241 haaqafevda ilvldqerly nelvrdmpdf vkvvfvpksg gvversqaqr veardqgvre
      301 yfygsrtply phsfevkwse arlykigapv lpascmplgm kaednltklv avtpgpnllh
      361 hllsvsfads peddvvqtnv agfvcvtnvd verqtftvls pqprplpntv lllsdiqfmd
      421 sh
//
LOCUS       XP_034190686             334 aa            linear   INV 03-SEP-2025
DEFINITION  corticotropin releasing hormone binding protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034190686
VERSION     XP_034190686.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334795.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190686.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..334
                     /product="corticotropin releasing hormone binding protein"
                     /calculated_mol_wt=37290
     Region          26..319
                     /region_name="CRF-BP"
                     /note="Corticotropin-releasing factor binding protein
                     (CRF-BP); pfam05428"
                     /db_xref="CDD:461650"
     CDS             1..334
                     /gene="crh-BP"
                     /coded_by="XM_034334795.2:18..1022"
                     /db_xref="GeneID:117608980"
ORIGIN      
        1 mslvgsdrrl trlllvvvgl snvvsavikg ntdyqqyqqq fpgerfskdf yrtdfknglf
       61 ntyrrfkpvt dcmfvtsepg sfiytsesnd ekvcgiyfla gpdekvevnf isfdvpcehh
      121 glvsvvdgwe lngevfpsei dhrlplkqrs tefcgknvgm krtfvssqnv aviqyripka
      181 gkgftllarf iknprpcnvl atrisepftl rnygrrinct yvalypgnvq vialevgvsn
      241 flgsvrttet gtlqkceets pndrvviggs ygldtskvqi vdsicgidsk pdyhelveyg
      301 vtsvrlissg ffensvtvei qplkddlfdt nisi
//
LOCUS       XP_034190257             209 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab8 [Osmia lignaria lignaria].
ACCESSION   XP_034190257
VERSION     XP_034190257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="RAS oncogene family member Rab8"
                     /calculated_mol_wt=23654
     Region          6..172
                     /region_name="Rab8_Rab10_Rab13_like"
                     /note="Rab GTPase families 8, 10, 13 (Rab8, Rab10, Rab13);
                     cd01867"
                     /db_xref="CDD:206659"
     Site            6..10
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206659"
     Site            15..22
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206659"
     Site            order(17..23,33..36,39..40,66,121..122,124..125,152..153)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206659"
     Site            order(23..35,37..39)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206659"
     Site            order(33,38..46)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206659"
     Site            order(36,38,42..49,56,58)
                     /site_type="other"
                     /note="GEF interaction site [polypeptide binding]"
                     /db_xref="CDD:206659"
     Site            40
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206659"
     Site            order(41,43..45,60,62,69..70,73,77,79..82,169)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206659"
     Site            order(41..42,44,62..63,70,72,74..76)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206659"
     Site            41..45
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206659"
     Site            58..62
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206659"
     Site            63..66
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206659"
     Site            order(66,68..78)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206659"
     Site            69..74
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206659"
     Site            77..81
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206659"
     Site            86..91
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206659"
     Site            109..118
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206659"
     Site            121..124
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206659"
     Site            151..153
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206659"
     Site            167..172
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206659"
     CDS             1..209
                     /gene="Rab8"
                     /coded_by="XM_034334366.2:376..1005"
                     /db_xref="GeneID:117608776"
ORIGIN      
        1 maktydylfk llligdsgvg ktcvlfrfse dafnttfist igidfkirti eldgkkiklq
       61 iwdtagqerf rtittayyrg amgimlvydv tneksfenik nwirnieena sadvekmllg
      121 nkceltekrq vskergeqla veygikfmet sakssinvee afytlardik akmekkleas
      181 nppkggghql kasepqrkpp swlarcsil
//
LOCUS       XP_076545863            2896 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545863
VERSION     XP_076545863.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689748.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2896
                     /product="uncharacterized protein LOC117606476 isoform X1"
                     /calculated_mol_wt=316433
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2896
                     /gene="LOC117606476"
                     /coded_by="XM_076689748.1:2410..11100"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs katapvpaer tvvgsasvig
     2461 ggphaleppt ahqqrpenal dalldelqtf srpssqlghv qtsvrgeapa gavttnvgtp
     2521 arapcisdig rkgsidsssg tviaalaptg aaanvaggtl rrlhsypsss dtdtsppiar
     2581 lqvslqdqqp slptlpqgfv pgqkppvper naellqlata rrvppppppr tssrsplasp
     2641 tspqlpprnh scnlqtgnat lrrppargtl pikegkppma lpaeavsald arpsllhnng
     2701 stgsqtlavl stsnsssces vnsqeglqie kerqeqldqr hqellrkqka lqeqyarlqq
     2761 lqrntagltt vppappdllk ktgsesnlla kmglglsaas sgsltslagk piapipqeat
     2821 raeqpgdqrn tasvnsstsi riattatast esngadqvpn saaisngaas attapvtatp
     2881 sttttttski yetdil
//
LOCUS       XP_076545864            2896 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545864
VERSION     XP_076545864.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689749.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2896
                     /product="uncharacterized protein LOC117606476 isoform X1"
                     /calculated_mol_wt=316433
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2896
                     /gene="LOC117606476"
                     /coded_by="XM_076689749.1:908..9598"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs katapvpaer tvvgsasvig
     2461 ggphaleppt ahqqrpenal dalldelqtf srpssqlghv qtsvrgeapa gavttnvgtp
     2521 arapcisdig rkgsidsssg tviaalaptg aaanvaggtl rrlhsypsss dtdtsppiar
     2581 lqvslqdqqp slptlpqgfv pgqkppvper naellqlata rrvppppppr tssrsplasp
     2641 tspqlpprnh scnlqtgnat lrrppargtl pikegkppma lpaeavsald arpsllhnng
     2701 stgsqtlavl stsnsssces vnsqeglqie kerqeqldqr hqellrkqka lqeqyarlqq
     2761 lqrntagltt vppappdllk ktgsesnlla kmglglsaas sgsltslagk piapipqeat
     2821 raeqpgdqrn tasvnsstsi riattatast esngadqvpn saaisngaas attapvtatp
     2881 sttttttski yetdil
//
LOCUS       XP_076545865            2896 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545865
VERSION     XP_076545865.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689750.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2896
                     /product="uncharacterized protein LOC117606476 isoform X1"
                     /calculated_mol_wt=316433
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2896
                     /gene="LOC117606476"
                     /coded_by="XM_076689750.1:174..8864"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs katapvpaer tvvgsasvig
     2461 ggphaleppt ahqqrpenal dalldelqtf srpssqlghv qtsvrgeapa gavttnvgtp
     2521 arapcisdig rkgsidsssg tviaalaptg aaanvaggtl rrlhsypsss dtdtsppiar
     2581 lqvslqdqqp slptlpqgfv pgqkppvper naellqlata rrvppppppr tssrsplasp
     2641 tspqlpprnh scnlqtgnat lrrppargtl pikegkppma lpaeavsald arpsllhnng
     2701 stgsqtlavl stsnsssces vnsqeglqie kerqeqldqr hqellrkqka lqeqyarlqq
     2761 lqrntagltt vppappdllk ktgsesnlla kmglglsaas sgsltslagk piapipqeat
     2821 raeqpgdqrn tasvnsstsi riattatast esngadqvpn saaisngaas attapvtatp
     2881 sttttttski yetdil
//
LOCUS       XP_076545866            2891 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545866
VERSION     XP_076545866.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689751.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2891
                     /product="uncharacterized protein LOC117606476 isoform X2"
                     /calculated_mol_wt=315842
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2891
                     /gene="LOC117606476"
                     /coded_by="XM_076689751.1:2409..11084"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 ssktvadlkv rfpslqegmk gllssememv vraekflkee perlesalkr ckkltstlvt
     2401 lkrlasvqeq rlpnaatsvd aeetppitpt saqhskatap vpaertvvgs asvigggpha
     2461 lepptahqqr penaldalld elqtfsrpss qlghvqtsvr geapagavtt nvgtparapc
     2521 isdigrkgsi dsssgtviaa laptgaaanv aggtlrrlhs ypsssdtdts ppiarlqvsl
     2581 qdqqpslptl pqgfvpgqkp pvpernaell qlatarrvpp pppprtssrs plasptspql
     2641 pprnhscnlq tgnatlrrpp argtlpikeg kppmalpaea vsaldarpsl lhnngstgsq
     2701 tlavlstsns sscesvnsqe glqiekerqe qldqrhqell rkqkalqeqy arlqqlqrnt
     2761 aglttvppap pdllkktgse snllakmglg lsaassgslt slagkpiapi pqeatraeqp
     2821 gdqrntasvn sstsiriatt atastesnga dqvpnsaais ngaasattap vtatpstttt
     2881 ttskiyetdi l
//
LOCUS       XP_076545867            2889 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545867
VERSION     XP_076545867.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689752.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2889
                     /product="uncharacterized protein LOC117606476 isoform X3"
                     /calculated_mol_wt=315750
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2889
                     /gene="LOC117606476"
                     /coded_by="XM_076689752.1:2410..11079"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpd dsylrtdvkp pklgkdksvs feksvsfsdd ppdmnspkqh
     2161 spqhaadtkp tkpaiksstl prmssqerdr hkptpppkpa alvagqyvyr dlaltpemyn
     2221 qlrglqkkak dlrqevrnlr rmsqaqahti retildtfit iramllscgd aawdaekirl
     2281 sreedlyrqe mlrlvkdlte lentveelrg nvinrktrvn msdvenmali msksslsrfs
     2341 ktvadlkvrf pslqegmkgl lssememvvr aekflkeepe rlesalkrck kltstlvtlk
     2401 rlasvqeqrl pnaatsvdae etppitptsa qhskatapvp aertvvgsas vigggphale
     2461 pptahqqrpe naldalldel qtfsrpssql ghvqtsvrge apagavttnv gtparapcis
     2521 digrkgsids ssgtviaala ptgaaanvag gtlrrlhsyp sssdtdtspp iarlqvslqd
     2581 qqpslptlpq gfvpgqkppv pernaellql atarrvpppp pprtssrspl asptspqlpp
     2641 rnhscnlqtg natlrrppar gtlpikegkp pmalpaeavs aldarpsllh nngstgsqtl
     2701 avlstsnsss cesvnsqegl qiekerqeql dqrhqellrk qkalqeqyar lqqlqrntag
     2761 lttvppappd llkktgsesn llakmglgls aassgsltsl agkpiapipq eatraeqpgd
     2821 qrntasvnss tsiriattat astesngadq vpnsaaisng aasattapvt atpstttttt
     2881 skiyetdil
//
LOCUS       XP_076545868            2878 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545868
VERSION     XP_076545868.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689753.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2878
                     /product="uncharacterized protein LOC117606476 isoform X4"
                     /calculated_mol_wt=314672
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2878
                     /gene="LOC117606476"
                     /coded_by="XM_076689753.1:2410..11046"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pvsqpavtdd sylrtdvkpp klgkdksvsf eksvsfsddp pdmnspkqhs pqhaadtkpt
     2161 kpaiksstlp rmssqerdrh kptpppkpaa lvagqyvyrd laltpemynq lrglqkkakd
     2221 lrqevrnlrr msqaqahtir etildtfiti ramllscgda awdaekirls reedlyrqem
     2281 lrlvkdltel entveelrgn vinrktrvnm sdvenmalim sksslsrfsk tvadlkvrfp
     2341 slqegmkgll ssememvvra ekflkeeper lesalkrckk ltstlvtlkr lasvqeqrlp
     2401 naatsvdaee tppitptsaq hskatapvpa ertvvgsasv igggphalep ptahqqrpen
     2461 aldalldelq tfsrpssqlg hvqtsvrgea pagavttnvg tparapcisd igrkgsidss
     2521 sgtviaalap tgaaanvagg tlrrlhsyps ssdtdtsppi arlqvslqdq qpslptlpqg
     2581 fvpgqkppvp ernaellqla tarrvppppp prtssrspla sptspqlppr nhscnlqtgn
     2641 atlrrpparg tlpikegkpp malpaeavsa ldarpsllhn ngstgsqtla vlstsnsssc
     2701 esvnsqeglq iekerqeqld qrhqellrkq kalqeqyarl qqlqrntagl ttvppappdl
     2761 lkktgsesnl lakmglglsa assgsltsla gkpiapipqe atraeqpgdq rntasvnsst
     2821 siriattata stesngadqv pnsaaisnga asattapvta tpstttttts kiyetdil
//
LOCUS       XP_076545869            2875 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545869
VERSION     XP_076545869.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689754.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2875
                     /product="uncharacterized protein LOC117606476 isoform X5"
                     /calculated_mol_wt=314206
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2875
                     /gene="LOC117606476"
                     /coded_by="XM_076689754.1:2410..11037"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haerdrhkpt pppkpaalva gqyvyrdlal tpemynqlrg lqkkakdlrq
     2221 evrnlrrmsq aqahtireti ldtfitiram llscgdaawd aekirlsree dlyrqemlrl
     2281 vkdltelent veelrgnvin rktrvnmsdv enmalimsks slsrfsktva dlkvrfpslq
     2341 egmkgllsse memvvraekf lkeeperles alkrckklts tlvtlkrlas vqeqrlpnaa
     2401 tsvdaeetpp itptsaqhsk atapvpaert vvgsasvigg gphaleppta hqqrpenald
     2461 alldelqtfs rpssqlghvq tsvrgeapag avttnvgtpa rapcisdigr kgsidsssgt
     2521 viaalaptga aanvaggtlr rlhsypsssd tdtsppiarl qvslqdqqps lptlpqgfvp
     2581 gqkppvpern aellqlatar rvpppppprt ssrsplaspt spqlpprnhs cnlqtgnatl
     2641 rrppargtlp ikegkppmal paeavsalda rpsllhnngs tgsqtlavls tsnssscesv
     2701 nsqeglqiek erqeqldqrh qellrkqkal qeqyarlqql qrntaglttv ppappdllkk
     2761 tgsesnllak mglglsaass gsltslagkp iapipqeatr aeqpgdqrnt asvnsstsir
     2821 iattataste sngadqvpns aaisngaasa ttapvtatps ttttttskiy etdil
//
LOCUS       XP_076545870            2871 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545870
VERSION     XP_076545870.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689755.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2871
                     /product="uncharacterized protein LOC117606476 isoform X6"
                     /calculated_mol_wt=313989
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2871
                     /gene="LOC117606476"
                     /coded_by="XM_076689755.1:2410..11025"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pddsylrtdv kppklgkdks vsfeksvsfs ddppdmnspk qhspqhaadt kptkpaikss
     2161 tlprmssqer drhkptpppk paalvagqyv yrdlaltpem ynqlrglqkk akdlrqevrn
     2221 lrrmsqaqah tiretildtf itiramllsc gdaawdaeki rlsreedlyr qemlrlvkdl
     2281 telentveel rgnvinrktr vnmsdvenma limsksslsr fsktvadlkv rfpslqegmk
     2341 gllssememv vraekflkee perlesalkr ckkltstlvt lkrlasvqeq rlpnaatsvd
     2401 aeetppitpt saqhskatap vpaertvvgs asvigggpha lepptahqqr penaldalld
     2461 elqtfsrpss qlghvqtsvr geapagavtt nvgtparapc isdigrkgsi dsssgtviaa
     2521 laptgaaanv aggtlrrlhs ypsssdtdts ppiarlqvsl qdqqpslptl pqgfvpgqkp
     2581 pvpernaell qlatarrvpp pppprtssrs plasptspql pprnhscnlq tgnatlrrpp
     2641 argtlpikeg kppmalpaea vsaldarpsl lhnngstgsq tlavlstsns sscesvnsqe
     2701 glqiekerqe qldqrhqell rkqkalqeqy arlqqlqrnt aglttvppap pdllkktgse
     2761 snllakmglg lsaassgslt slagkpiapi pqeatraeqp gdqrntasvn sstsiriatt
     2821 atastesnga dqvpnsaais ngaasattap vtatpstttt ttskiyetdi l
//
LOCUS       XP_076545871            2866 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545871
VERSION     XP_076545871.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689756.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2866
                     /product="uncharacterized protein LOC117606476 isoform X7"
                     /calculated_mol_wt=313398
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2866
                     /gene="LOC117606476"
                     /coded_by="XM_076689756.1:2408..11008"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pddsylrtdv kppklgkdks vsfeksvsfs ddppdmnspk qhspqhaadt kptkpaikss
     2161 tlprmssqer drhkptpppk paalvagqyv yrdlaltpem ynqlrglqkk akdlrqevrn
     2221 lrrmsqaqah tiretildtf itiramllsc gdaawdaeki rlsreedlyr qemlrlvkdl
     2281 telentveel rgnvinrktr vnmsdvenma limskssktv adlkvrfpsl qegmkgllss
     2341 ememvvraek flkeeperle salkrckklt stlvtlkrla svqeqrlpna atsvdaeetp
     2401 pitptsaqhs katapvpaer tvvgsasvig ggphaleppt ahqqrpenal dalldelqtf
     2461 srpssqlghv qtsvrgeapa gavttnvgtp arapcisdig rkgsidsssg tviaalaptg
     2521 aaanvaggtl rrlhsypsss dtdtsppiar lqvslqdqqp slptlpqgfv pgqkppvper
     2581 naellqlata rrvppppppr tssrsplasp tspqlpprnh scnlqtgnat lrrppargtl
     2641 pikegkppma lpaeavsald arpsllhnng stgsqtlavl stsnsssces vnsqeglqie
     2701 kerqeqldqr hqellrkqka lqeqyarlqq lqrntagltt vppappdllk ktgsesnlla
     2761 kmglglsaas sgsltslagk piapipqeat raeqpgdqrn tasvnsstsi riattatast
     2821 esngadqvpn saaisngaas attapvtatp sttttttski yetdil
//
LOCUS       XP_076545872            2855 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545872
VERSION     XP_076545872.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689757.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2855
                     /product="uncharacterized protein LOC117606476 isoform X8"
                     /calculated_mol_wt=312175
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2855
                     /gene="LOC117606476"
                     /coded_by="XM_076689757.1:2409..10976"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pdksvsfeks vsfsddppdm nspkqhspqh aadtkptkpa iksstlprms sqerdrhkpt
     2161 pppkpaalva gqyvyrdlal tpemynqlrg lqkkakdlrq evrnlrrmsq aqahtireti
     2221 ldtfitiram llscgdaawd aekirlsree dlyrqemlrl vkdltelent veelrgnvin
     2281 rktrvnmsdv enmalimsks slsrfsktva dlkvrfpslq egmkgllsse memvvraekf
     2341 lkeeperles alkrckklts tlvtlkrlas vqeqrlpnaa tsvdaeetpp itptsaqhsk
     2401 atapvpaert vvgsasvigg gphaleppta hqqrpenald alldelqtfs rpssqlghvq
     2461 tsvrgeapag avttnvgtpa rapcisdigr kgsidsssgt viaalaptga aanvaggtlr
     2521 rlhsypsssd tdtsppiarl qvslqdqqps lptlpqgfvp gqkppvpern aellqlatar
     2581 rvpppppprt ssrsplaspt spqlpprnhs cnlqtgnatl rrppargtlp ikegkppmal
     2641 paeavsalda rpsllhnngs tgsqtlavls tsnssscesv nsqeglqiek erqeqldqrh
     2701 qellrkqkal qeqyarlqql qrntaglttv ppappdllkk tgsesnllak mglglsaass
     2761 gsltslagkp iapipqeatr aeqpgdqrnt asvnsstsir iattataste sngadqvpns
     2821 aaisngaasa ttapvtatps ttttttskiy etdil
//
LOCUS       XP_076545873            2854 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545873
VERSION     XP_076545873.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689758.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2854
                     /product="uncharacterized protein LOC117606476 isoform X9"
                     /calculated_mol_wt=312282
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2854
                     /gene="LOC117606476"
                     /coded_by="XM_076689758.1:2410..10974"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpdrlhg esgymssper
     1921 gggvgsgtgr yppgpysags syedpyysqy sgtvtpvide easdtellee syslygvkpp
     1981 grppsgpprs pfppgapppl ppggqsydat rirvehmerq lanltglvqk althaphtsp
     2041 sprdylqvpa grdpyargpg agdefdkhss sssaslpvsq pavtddsylr tdvkppklgk
     2101 dksvsfeksv sfsddppdmn spkqhspqha adtkptkpai ksstlprmss qerdrhkptp
     2161 ppkpaalvag qyvyrdlalt pemynqlrgl qkkakdlrqe vrnlrrmsqa qahtiretil
     2221 dtfitiraml lscgdaawda ekirlsreed lyrqemlrlv kdltelentv eelrgnvinr
     2281 ktrvnmsdve nmalimskss lsrfsktvad lkvrfpslqe gmkgllssem emvvraekfl
     2341 keeperlesa lkrckkltst lvtlkrlasv qeqrlpnaat svdaeetppi tptsaqhska
     2401 tapvpaertv vgsasviggg phalepptah qqrpenalda lldelqtfsr pssqlghvqt
     2461 svrgeapaga vttnvgtpar apcisdigrk gsidsssgtv iaalaptgaa anvaggtlrr
     2521 lhsypsssdt dtsppiarlq vslqdqqpsl ptlpqgfvpg qkppvperna ellqlatarr
     2581 vpppppprts srsplaspts pqlpprnhsc nlqtgnatlr rppargtlpi kegkppmalp
     2641 aeavsaldar psllhnngst gsqtlavlst snssscesvn sqeglqieke rqeqldqrhq
     2701 ellrkqkalq eqyarlqqlq rntaglttvp pappdllkkt gsesnllakm glglsaassg
     2761 sltslagkpi apipqeatra eqpgdqrnta svnsstsiri attatastes ngadqvpnsa
     2821 aisngaasat tapvtatpst tttttskiye tdil
//
LOCUS       XP_076545874            2848 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545874
VERSION     XP_076545874.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689759.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2848
                     /product="uncharacterized protein LOC117606476 isoform
                     X10"
                     /calculated_mol_wt=311489
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2848
                     /gene="LOC117606476"
                     /coded_by="XM_076689759.1:2409..10955"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deearirveh merqlanltg lvqkalthap htspsprdyl
     2041 qvpagrdpya rgpgagdefd khsssssasl pvsqpavtdd sylrtdvkpp klgkdksvsf
     2101 eksvsfsddp pdmnspkqhs pqhaadtkpt kpaiksstlp rmssqerdrh kptpppkpaa
     2161 lvagqyvyrd laltpemynq lrglqkkakd lrqevrnlrr msqaqahtir etildtfiti
     2221 ramllscgda awdaekirls reedlyrqem lrlvkdltel entveelrgn vinrktrvnm
     2281 sdvenmalim sksslsrfsk tvadlkvrfp slqegmkgll ssememvvra ekflkeeper
     2341 lesalkrckk ltstlvtlkr lasvqeqrlp naatsvdaee tppitptsaq hskatapvpa
     2401 ertvvgsasv igggphalep ptahqqrpen aldalldelq tfsrpssqlg hvqtsvrgea
     2461 pagavttnvg tparapcisd igrkgsidss sgtviaalap tgaaanvagg tlrrlhsyps
     2521 ssdtdtsppi arlqvslqdq qpslptlpqg fvpgqkppvp ernaellqla tarrvppppp
     2581 prtssrspla sptspqlppr nhscnlqtgn atlrrpparg tlpikegkpp malpaeavsa
     2641 ldarpsllhn ngstgsqtla vlstsnsssc esvnsqeglq iekerqeqld qrhqellrkq
     2701 kalqeqyarl qqlqrntagl ttvppappdl lkktgsesnl lakmglglsa assgsltsla
     2761 gkpiapipqe atraeqpgdq rntasvnsst siriattata stesngadqv pnsaaisnga
     2821 asattapvta tpstttttts kiyetdil
//
LOCUS       XP_076545875            2834 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X11 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545875
VERSION     XP_076545875.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689760.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2834
                     /product="uncharacterized protein LOC117606476 isoform
                     X11"
                     /calculated_mol_wt=309948
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2834
                     /gene="LOC117606476"
                     /coded_by="XM_076689760.1:2409..10913"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pdksvsfeks vsfsddppdm nspkqhspqh aerdrhkptp ppkpaalvag qyvyrdlalt
     2161 pemynqlrgl qkkakdlrqe vrnlrrmsqa qahtiretil dtfitiraml lscgdaawda
     2221 ekirlsreed lyrqemlrlv kdltelentv eelrgnvinr ktrvnmsdve nmalimskss
     2281 lsrfsktvad lkvrfpslqe gmkgllssem emvvraekfl keeperlesa lkrckkltst
     2341 lvtlkrlasv qeqrlpnaat svdaeetppi tptsaqhska tapvpaertv vgsasviggg
     2401 phalepptah qqrpenalda lldelqtfsr pssqlghvqt svrgeapaga vttnvgtpar
     2461 apcisdigrk gsidsssgtv iaalaptgaa anvaggtlrr lhsypsssdt dtsppiarlq
     2521 vslqdqqpsl ptlpqgfvpg qkppvperna ellqlatarr vpppppprts srsplaspts
     2581 pqlpprnhsc nlqtgnatlr rppargtlpi kegkppmalp aeavsaldar psllhnngst
     2641 gsqtlavlst snssscesvn sqeglqieke rqeqldqrhq ellrkqkalq eqyarlqqlq
     2701 rntaglttvp pappdllkkt gsesnllakm glglsaassg sltslagkpi apipqeatra
     2761 eqpgdqrnta svnsstsiri attatastes ngadqvpnsa aisngaasat tapvtatpst
     2821 tttttskiye tdil
//
LOCUS       XP_076545876            2804 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X12 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545876
VERSION     XP_076545876.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689761.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2804
                     /product="uncharacterized protein LOC117606476 isoform
                     X12"
                     /calculated_mol_wt=306637
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2804
                     /gene="LOC117606476"
                     /coded_by="XM_076689761.1:2410..10824"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 perdrhkptp ppkpaalvag qyvyrdlalt pemynqlrgl qkkakdlrqe vrnlrrmsqa
     2161 qahtiretil dtfitiraml lscgdaawda ekirlsreed lyrqemlrlv kdltelentv
     2221 eelrgnvinr ktrvnmsdve nmalimskss lsrfsktvad lkvrfpslqe gmkgllssem
     2281 emvvraekfl keeperlesa lkrckkltst lvtlkrlasv qeqrlpnaat svdaeetppi
     2341 tptsaqhska tapvpaertv vgsasviggg phalepptah qqrpenalda lldelqtfsr
     2401 pssqlghvqt svrgeapaga vttnvgtpar apcisdigrk gsidsssgtv iaalaptgaa
     2461 anvaggtlrr lhsypsssdt dtsppiarlq vslqdqqpsl ptlpqgfvpg qkppvperna
     2521 ellqlatarr vpppppprts srsplaspts pqlpprnhsc nlqtgnatlr rppargtlpi
     2581 kegkppmalp aeavsaldar psllhnngst gsqtlavlst snssscesvn sqeglqieke
     2641 rqeqldqrhq ellrkqkalq eqyarlqqlq rntaglttvp pappdllkkt gsesnllakm
     2701 glglsaassg sltslagkpi apipqeatra eqpgdqrnta svnsstsiri attatastes
     2761 ngadqvpnsa aisngaasat tapvtatpst tttttskiye tdil
//
LOCUS       XP_076545877            2794 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X13 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545877
VERSION     XP_076545877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689762.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2794
                     /product="uncharacterized protein LOC117606476 isoform
                     X13"
                     /calculated_mol_wt=306065
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2794
                     /gene="LOC117606476"
                     /coded_by="XM_076689762.1:2406..10790"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpdrlhg esgymssper
     1921 gggvgsgtgr yppgpysags syedpyysqy sgtvtpvide earirvehme rqlanltglv
     1981 qkalthapht spsprdylqv pagrdpyarg pgagdefdkh sssssaslpd dsylrtdvkp
     2041 pklgkdksvs feksvsfsdd ppdmnspkqh spqhaadtkp tkpaiksstl prmssqerdr
     2101 hkptpppkpa alvagqyvyr dlaltpemyn qlrglqkkak dlrqevrnlr rmsqaqahti
     2161 retildtfit iramllscgd aawdaekirl sreedlyrqe mlrlvkdlte lentveelrg
     2221 nvinrktrvn msdvenmali mskssktvad lkvrfpslqe gmkgllssem emvvraekfl
     2281 keeperlesa lkrckkltst lvtlkrlasv qeqrlpnaat svdaeetppi tptsaqhska
     2341 tapvpaertv vgsasviggg phalepptah qqrpenalda lldelqtfsr pssqlghvqt
     2401 svrgeapaga vttnvgtpar apcisdigrk gsidsssgtv iaalaptgaa anvaggtlrr
     2461 lhsypsssdt dtsppiarlq vslqdqqpsl ptlpqgfvpg qkppvperna ellqlatarr
     2521 vpppppprts srsplaspts pqlpprnhsc nlqtgnatlr rppargtlpi kegkppmalp
     2581 aeavsaldar psllhnngst gsqtlavlst snssscesvn sqeglqieke rqeqldqrhq
     2641 ellrkqkalq eqyarlqqlq rntaglttvp pappdllkkt gsesnllakm glglsaassg
     2701 sltslagkpi apipqeatra eqpgdqrnta svnsstsiri attatastes ngadqvpnsa
     2761 aisngaasat tapvtatpst tttttskiye tdil
//
LOCUS       XP_034184848            2608 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X14 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184848
VERSION     XP_034184848.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328957.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184848.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2608
                     /product="uncharacterized protein LOC117606476 isoform
                     X14"
                     /calculated_mol_wt=286097
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>2135
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; smart00806"
                     /db_xref="CDD:214826"
     CDS             1..2608
                     /gene="LOC117606476"
                     /coded_by="XM_034328957.2:2409..10235"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttperdrh kptpppkpaa
     1921 lvagqyvyrd laltpemynq lrglqkkakd lrqevrnlrr msqaqahtir etildtfiti
     1981 ramllscgda awdaekirls reedlyrqem lrlvkdltel entveelrgn vinrktrvnm
     2041 sdvenmalim sksslsrfsk tvadlkvrfp slqegmkgll ssememvvra ekflkeeper
     2101 lesalkrckk ltstlvtlkr lasvqeqrlp naatsvdaee tppitptsaq hskatapvpa
     2161 ertvvgsasv igggphalep ptahqqrpen aldalldelq tfsrpssqlg hvqtsvrgea
     2221 pagavttnvg tparapcisd igrkgsidss sgtviaalap tgaaanvagg tlrrlhsyps
     2281 ssdtdtsppi arlqvslqdq qpslptlpqg fvpgqkppvp ernaellqla tarrvppppp
     2341 prtssrspla sptspqlppr nhscnlqtgn atlrrpparg tlpikegkpp malpaeavsa
     2401 ldarpsllhn ngstgsqtla vlstsnsssc esvnsqeglq iekerqeqld qrhqellrkq
     2461 kalqeqyarl qqlqrntagl ttvppappdl lkktgsesnl lakmglglsa assgsltsla
     2521 gkpiapipqe atraeqpgdq rntasvnsst siriattata stesngadqv pnsaaisnga
     2581 asattapvta tpstttttts kiyetdil
//
LOCUS       XP_076545878            2516 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X15 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545878
VERSION     XP_076545878.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689763.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2516
                     /product="uncharacterized protein LOC117606476 isoform
                     X15"
                     /calculated_mol_wt=277872
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2516
                     /gene="LOC117606476"
                     /coded_by="XM_076689763.1:2411..9961"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs kygwkwstnf keaiqtshdf
     2461 lerrsspgld tappsepare kvgprrdald apapcrpkes srdlassdtl vpihfl
//
LOCUS       XP_076545879            2501 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X16 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545879
VERSION     XP_076545879.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689764.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2501
                     /product="uncharacterized protein LOC117606476 isoform
                     X16"
                     /calculated_mol_wt=276190
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2501
                     /gene="LOC117606476"
                     /coded_by="XM_076689764.1:2411..9916"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs kygwkwstnf keaiqtsheq
     2461 lplcqrnals wgqrassgeg ptlsshpppi ssvrrthstl c
//
LOCUS       XP_076545880            2496 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X17 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545880
VERSION     XP_076545880.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689765.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2496
                     /product="uncharacterized protein LOC117606476 isoform
                     X17"
                     /calculated_mol_wt=275599
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2496
                     /gene="LOC117606476"
                     /coded_by="XM_076689765.1:2409..9899"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 ssktvadlkv rfpslqegmk gllssememv vraekflkee perlesalkr ckkltstlvt
     2401 lkrlasvqeq rlpnaatsvd aeetppitpt saqhskygwk wstnfkeaiq tsheqlplcq
     2461 rnalswgqra ssgegptlss hpppissvrr thstlc
//
LOCUS       XP_076545881            2465 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X18 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545881
VERSION     XP_076545881.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689766.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2465
                     /product="uncharacterized protein LOC117606476 isoform
                     X18"
                     /calculated_mol_wt=272286
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2465
                     /gene="LOC117606476"
                     /coded_by="XM_076689766.1:2410..9807"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs kitriasdag yvnekkkrne
     2461 keknl
//
LOCUS       XP_076545882            2462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X19 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545882
VERSION     XP_076545882.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689767.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2462
                     /product="uncharacterized protein LOC117606476 isoform
                     X19"
                     /calculated_mol_wt=271771
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2462
                     /gene="LOC117606476"
                     /coded_by="XM_076689767.1:2410..9798"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs kltntgqqsm pnvkepqtst
     2461 dl
//
LOCUS       XP_076545883            2462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X19 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545883
VERSION     XP_076545883.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689768.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2462
                     /product="uncharacterized protein LOC117606476 isoform
                     X19"
                     /calculated_mol_wt=271771
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2462
                     /gene="LOC117606476"
                     /coded_by="XM_076689768.1:2410..9798"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs kltntgqqsm pnvkepqtst
     2461 dl
//
LOCUS       XP_076545884            2462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X19 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545884
VERSION     XP_076545884.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689769.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2462
                     /product="uncharacterized protein LOC117606476 isoform
                     X19"
                     /calculated_mol_wt=271771
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2462
                     /gene="LOC117606476"
                     /coded_by="XM_076689769.1:2410..9798"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs kltntgqqsm pnvkepqtst
     2461 dl
//
LOCUS       XP_076545885            2459 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606476 isoform X20 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545885
VERSION     XP_076545885.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689770.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2459
                     /product="uncharacterized protein LOC117606476 isoform
                     X20"
                     /calculated_mol_wt=271680
     Region          9..>348
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1061..1523
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1724..>1801
                     /region_name="AIP3"
                     /note="Actin interacting protein 3; pfam03915"
                     /db_xref="CDD:427585"
     CDS             1..2459
                     /gene="LOC117606476"
                     /coded_by="XM_076689770.1:2410..9789"
                     /db_xref="GeneID:117606476"
ORIGIN      
        1 megedvgddk lskkeepglr vlsvdkvtve kivfesssgs revlltknet srklsiksel
       61 srsedegrkn raslpedila anskgaiekr ikpikltkgp sigsivvdvp skleinesns
      121 kkaeekakde dnqedrrene gteakdrlek srsnikvlqe nlttkidant veryrrklqe
      181 lrencahkll dsnanekfte vafdeeflqk hnldrrlkie edrspegele qesdelltkd
      241 tatakpsysk kvsevekrws geflenrltr rssesskssy veefgsvsse esvagdrdsp
      301 rferekrnte veesgresfg rqtseevlad stvstldsds cledrikale eeesvdrkde
      361 veddegedld vvrrrrrskl layeradrwn eslkrakvss gcstdsidst rsrkdgyasl
      421 vrelameaaa gtsrvkkeek qkrklglrrl lpgffspkds rkdykkkkes kerrrhedrh
      481 faryqqngny trspdtmnln edikrnvkld nslngsiiee rldeikrelf pdqcpitstp
      541 dhlardeeqs llrdrsgaar aygidsslss iapddrwner sghlgispdi rkfeqrqkre
      601 efdqryggqq lerkhslqes nppnrlcffq rnhgpsgris appserylvr praihpidrp
      661 lpaiprsrvd ssnyenypee dnqprpigye rsgaytadka eysnkstlye negspgglil
      721 ksvsaqvkit rqpignqnqk raplvgrspk ylssgssqks gdygdssctp nssqksefsp
      781 tssksgeyyl nsprnsgspn drdfdeenps sregiyenen enspsrsstr caderiydet
      841 psspskppir spmddsldkt dsspgrgcsk ggrpmsprtn adkqsidget gktgartvps
      901 rrsqpneqil iaspkreiay ecriprplne srlcavespv rmmntphrlt gvtpleprnq
      961 dpsgnreppa skmieddypg gggglaeiar pepvyghhrn qpvagqnggs qaederrkdc
     1021 ddrvawvqgr atspatspkr neatsgerne spvtenhrqg qnqppsrspr svaplllqea
     1081 qrpriagrek lygtagpgrv dpprvlqpqa qqrqprsptp rslteplylr qipestygqr
     1141 enaavgplsp skqetrqhle afywqqktle asrktvapps ananprqigr kidlpevrea
     1201 vywqqlkkld eeqqrriyeq nlmeesscck agpkipsars vsqrqvsvps pagamnlslt
     1261 igygepgpwa gnvcrskgnp sgkpplmqsq kgpnqpvliv rpqqairerr eaampskpvd
     1321 ssrrpetqrs ksasphfhrg dapqivprkl dapsiyeeev aavndvegks apppifkrgs
     1381 liggesaeyg tgggakrvsf snqsttvgrd lpsgswptkh gtapepptrr hrsedsvsdt
     1441 dsvflhqerr daaagpcpdv eydadrplpp lpndvapanp sgsgstprsn aygevrwnpp
     1501 rpdprraisp hrmlrpkeee wnsdgkgrqs ndiggsgrge geagsneipg grrggggggg
     1561 igvggggsvl gvgggggggg sggggpggrs gsvgggrsrd eprrhtlsgd hqpslhhqqf
     1621 naaqqlhplh phhlppphgq ygtpptrhtt mdlemgtksr qrksplprgy pppsstmlld
     1681 edpgimseve tsstgfrrgg kqrsslpvvr npsktlerpl glvflqyrne tkrallpnei
     1741 tsidtvkalf vrsfpkqltm eyldsphvkv yihdsnkdmf yeledlrshl rdirdrsvlr
     1801 lfestdgvtg msgplgipgt gaglpphwed qsyfsepefd seyqhqhihk sktaknstsg
     1861 ssgyyvggss tlprggpvmr ayspaassvv gpsstptqpk plttpggggv ppakplrsyq
     1921 cgksplgslg gsarfsrdss vslysiadrl hgesgymssp ergggvgsgt gryppgpysa
     1981 gssyedpyys qysgtvtpvi deeasdtell eesyslygvk ppgrppsgpp rspfppgapp
     2041 plppggqsyd atrirvehme rqlanltglv qkalthapht spsprdylqv pagrdpyarg
     2101 pgagdefdkh sssssaslpv sqpavtddsy lrtdvkppkl gkdksvsfek svsfsddppd
     2161 mnspkqhspq haadtkptkp aiksstlprm ssqerdrhkp tpppkpaalv agqyvyrdla
     2221 ltpemynqlr glqkkakdlr qevrnlrrms qaqahtiret ildtfitira mllscgdaaw
     2281 daekirlsre edlyrqemlr lvkdltelen tveelrgnvi nrktrvnmsd venmalimsk
     2341 sslsrfsktv adlkvrfpsl qegmkgllss ememvvraek flkeeperle salkrckklt
     2401 stlvtlkrla svqeqrlpna atsvdaeetp pitptsaqhs kygwkwstnf keaiqtshd
//
LOCUS       XP_034184859             993 aa            linear   INV 03-SEP-2025
DEFINITION  glycine dehydrogenase (decarboxylating), mitochondrial [Osmia
            lignaria lignaria].
ACCESSION   XP_034184859
VERSION     XP_034184859.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328968.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..993
                     /product="glycine dehydrogenase (decarboxylating),
                     mitochondrial"
                     /calculated_mol_wt=110126
     Region          40..993
                     /region_name="PLN02414"
                     /note="glycine dehydrogenase (decarboxylating)"
                     /db_xref="CDD:178035"
     CDS             1..993
                     /gene="LOC117606477"
                     /coded_by="XM_034328968.2:112..3093"
                     /db_xref="GeneID:117606477"
ORIGIN      
        1 msytlkmtkc sklfnrsnvi qqtrgltlsn lrngkaeqfl sqqdefqvrh igpreheqlq
       61 mlktigfesl eeltktaipa kilykeelki eqpvteyell kkvtkisekn dvwrsyigmg
      121 yhnccvphti mrnifenpgw ttqytpyqpe isqgrlegll nyqtmicdlt gmevanasll
      181 degtaaaeal alayrsnkrk klfvsdkvhp qtisviatra tslglnfeig dvqqvdtsak
      241 diagillqyp dttgsiytfe divkrahadg tlvcaatdll alavlkppse fgvdvcvgts
      301 qrfgvplgyg gphagffacr qklvrlmpgr migvtkdasg rdayrlalqt reqhirrdka
      361 tsnictaqal lanmsamyav yhgpegirni ankvhvltla lakglenagn kieneyffdt
      421 ikvlpktpik tiqenanafk vnfryyndgv gisldettsi qnvndiykif sidttveevc
      481 qeescmnksl skshfartsp ylqhpvfnsh qsetrivrym kslenkdvsl vhsmiplgsc
      541 tmklnsttem mpcslrgfte ihpfvpieqa rgyqqlftel eqdlcaitgy dnisfqpnsg
      601 aqgeyaglra iqcyhesngt khrqvclipi sahgtnpasa qmagmqvkpi fvqkdgsvdt
      661 vhltemidky retlsclmit ypstngvfee tisdicsmvh eaggqvyldg anmnaqvglc
      721 rpgdygsdvs hlnlhktfci phggggpgmg pigvkrhltp flpshpvidc lgngnngikq
      781 sgavsaapfg ssailpiswa yikmmgpkgl rkatqvailn anymskrlek yyktlykgkt
      841 glvahefild irefkktani eaadiakrlm dygfhaptms wpvagtlmie ptesedkkel
      901 drfcdalifi rkeisdieng kldnlnnplk maphtqeqvi sttwdrgysr elaafpapfv
      961 kggnkiwpsv griddiygdm nlfctcppvs ldq
//
LOCUS       XP_034184860             966 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606478 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184860
VERSION     XP_034184860.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328969.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184860.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..966
                     /product="uncharacterized protein LOC117606478 isoform X1"
                     /calculated_mol_wt=104481
     Region          <688..949
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..966
                     /gene="LOC117606478"
                     /coded_by="XM_034328969.2:423..3323"
                     /db_xref="GeneID:117606478"
ORIGIN      
        1 mwlkvallit cviactladd esrggavsva dfvtshklpe dvarpldqde edddvvdver
       61 kvvgkdgkns kkvlpkfesf dkkllepvrr fdfgdddkgn takrqvrvel enfgdtgvlq
      121 edgirrtdaf rsrvddqrds vipgvrvsne ypttmlpilt tpretsgfdf vpvniirndg
      181 ketsfrernf gdfsdvslvp agtnsriqvk kgpngkdyey eyvyyyydee denkagtads
      241 avtnsydvps rstsaprrgs sssnrnkyss gersstvepa snevipnrng sgngnrgrql
      301 aetedvseer lptntrfppr srsnhntata ttepsrsrgn rprpgldlvd sssfrthqeg
      361 pefpqvlpkg pvrflgvtpn eeseertstr grrpqrvqep apveevvedv papttvrrrp
      421 vaseldesnr sqplrssean dekeeeslpi tekqtskekq tssekgeeye etdsssvsps
      481 tpasiatetp tteypsssmd kvaldlyafv qqgqnnlvda sssepsadss sdgttlsnee
      541 adttdltavt dqsattianl eltttvlpts tpeltttlas ttsttttttt tttttepptt
      601 ttsqstigrg kfrrpgigga aaprnrfksn gsgsttttea paeptqrtrv rfgsngsngn
      661 ggfkrnrpgh kqpveedavq kestssvhae rpaannnrnr frgsggtrsi gstttsapsn
      721 gagstasgps gvsrpsfnkl ninrrrgrpt ttapntsees qetgketaqe avtapskstv
      781 rarlpttgar pvikhgarvn lrqkpgqstt ttsttpaave npeessveep agegteeeth
      841 eapaetsptp arsttvnpln klrnrnrlqv hpktttrspv pavsrrspll prrkvteppv
      901 aqtssqeeav seevdvsget epteptsget staastkhee trglggllap rrritprrpg
      961 qiisre
//
LOCUS       XP_034184861             936 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606478 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184861
VERSION     XP_034184861.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328970.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184861.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..936
                     /product="uncharacterized protein LOC117606478 isoform X2"
                     /calculated_mol_wt=101013
     Region          <658..919
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..936
                     /gene="LOC117606478"
                     /coded_by="XM_034328970.2:423..3233"
                     /db_xref="GeneID:117606478"
ORIGIN      
        1 mwlkvallit cviactladd esrggavsva dfvtshklpe dvarpldqde edddvvdver
       61 kvvgkdgkns kkvlpkfesf dkkllepvrr fdfgdddkgn takrqvrvel enfgdtgvlq
      121 edgirrtdaf rsrvddqrds vipgvrvsne ypttmlpilt tpretsgfdf vpvniirndg
      181 ketsfrernf gdfsdvslvp agtnsriqvk kgpngkdyey estsaprrgs sssnrnkyss
      241 gersstvepa snevipnrng sgngnrgrql aetedvseer lptntrfppr srsnhntata
      301 ttepsrsrgn rprpgldlvd sssfrthqeg pefpqvlpkg pvrflgvtpn eeseertstr
      361 grrpqrvqep apveevvedv papttvrrrp vaseldesnr sqplrssean dekeeeslpi
      421 tekqtskekq tssekgeeye etdsssvsps tpasiatetp tteypsssmd kvaldlyafv
      481 qqgqnnlvda sssepsadss sdgttlsnee adttdltavt dqsattianl eltttvlpts
      541 tpeltttlas ttsttttttt tttttepptt ttsqstigrg kfrrpgigga aaprnrfksn
      601 gsgsttttea paeptqrtrv rfgsngsngn ggfkrnrpgh kqpveedavq kestssvhae
      661 rpaannnrnr frgsggtrsi gstttsapsn gagstasgps gvsrpsfnkl ninrrrgrpt
      721 ttapntsees qetgketaqe avtapskstv rarlpttgar pvikhgarvn lrqkpgqstt
      781 ttsttpaave npeessveep agegteeeth eapaetsptp arsttvnpln klrnrnrlqv
      841 hpktttrspv pavsrrspll prrkvteppv aqtssqeeav seevdvsget epteptsget
      901 staastkhee trglggllap rrritprrpg qiisre
//
LOCUS       XP_034185444             965 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185444
VERSION     XP_034185444.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329553.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..965
                     /product="uncharacterized protein LOC117606727 isoform X1"
                     /calculated_mol_wt=110160
     Site            order(45..48,50..51,53..55,68..71,75,77..78,102..103,105,
                     131,133)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411024"
     Region          65..278
                     /region_name="DCAF15_WD40"
                     /note="DDB1-and CUL4-substrate receptor 15, WD repeat;
                     pfam14939"
                     /db_xref="CDD:464387"
     Site            order(157,175,177..178,186,247,249..250,252)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411024"
     Site            249..254
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411024"
     Region          <302..>551
                     /region_name="PLN03188"
                     /note="kinesin-12 family protein; Provisional"
                     /db_xref="CDD:215621"
     Region          <460..566
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          721..954
                     /region_name="DCAF15-CTD"
                     /note="C-terminal domain of DDB1- and CUL4-associated
                     factor 15; cd20913"
                     /db_xref="CDD:411023"
     Site            order(822,909,912..913,916)
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411023"
     Site            order(825,827,834,864,875..879,881,918..919,925..926)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411023"
     Site            order(902..903,905..906,909..910,913..914,917,920,929,
                     948..949)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411023"
     CDS             1..965
                     /gene="LOC117606727"
                     /coded_by="XM_034329553.2:1..2898"
                     /db_xref="GeneID:117606727"
ORIGIN      
        1 madasssdtt sdccnewfsd itsekseyii vgqkpclshr rskrhflqkl flreirgcls
       61 ahnyaseerl fatvpswrhl pllhvipksa leaghivmgl tqcgqfllty tytmdmsgnt
      121 slykyllhww aftpnhvark vaevtlfgny tiyrelsivi sqwplerskl vihglctnwl
      181 hlqptdrayl tittvpslen ckdclkvaas yeeegeelaa nwdgcvrcnc lqhgltvhtt
      241 yevispyprf ratvclnywn hvvvntgnfl hvlrvdldip ksknqktcdk qdtvvtdtvp
      301 ldmsdveeld ytkvveryec sdeklctpec aiqspiiehd yleepiklnd rlnrdslnts
      361 iinqsdcvcd inksadpttv kscecmdvse ckcrtaspis rteqqaisvr dkilqdfced
      421 msqelnigsd litlvkhpsc sprstpqrlp sdlkamttws spiltppsdi lktrnsessq
      481 ksirtfqrst spqpgasedn nltsvnsplh svsvspssss rlmsppvtrs frhpsprkrs
      541 slhspppivn stqsrtrpth klileaekay eftdeaqetc eklssfrkrr ladkkyefcd
      601 etedaenivp fkhirdqfkh rgcsihqips sptmspaltn gkkhwvdsdq setddlnmsq
      661 dqngvndltt vdsteknvlr plnqnqlpng cisrersgkh cfnssplvpk inlqyptikc
      721 tahfkrsyie lddemisvit dvedeetggy vsyqcvlpmh vhgsgyvqmq misnskaekl
      781 ivpcvsinql sfdietfshh iadwicmrfk krywhcsdyd ieiidvcals gdiicllimk
      841 iqaseinnqt qcsqerkqye vgckftwnid tsqyritdvl pmeevkpesw kpncidvpnf
      901 rtplwnptrr lapklrekiq qpyahtvrfl hnemtlmges itrlydldnl vefyitpmpn
      961 ypsie
//
LOCUS       XP_034185445             868 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606727 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185445
VERSION     XP_034185445.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329554.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..868
                     /product="uncharacterized protein LOC117606727 isoform X2"
                     /calculated_mol_wt=99123
     Region          1..181
                     /region_name="DCAF15_WD40"
                     /note="DDB1-and CUL4-substrate receptor 15, WD repeat;
                     pfam14939"
                     /db_xref="CDD:464387"
     Site            order(60,78,80..81,89,150,152..153,155)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411024"
     Site            152..157
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411024"
     Region          <205..>454
                     /region_name="PLN03188"
                     /note="kinesin-12 family protein; Provisional"
                     /db_xref="CDD:215621"
     Region          <363..469
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          624..857
                     /region_name="DCAF15-CTD"
                     /note="C-terminal domain of DDB1- and CUL4-associated
                     factor 15; cd20913"
                     /db_xref="CDD:411023"
     Site            order(725,812,815..816,819)
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411023"
     Site            order(728,730,737,767,778..782,784,821..822,828..829)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411023"
     Site            order(805..806,808..809,812..813,816..817,820,823,832,
                     851..852)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411023"
     CDS             1..868
                     /gene="LOC117606727"
                     /coded_by="XM_034329554.2:402..3008"
                     /db_xref="GeneID:117606727"
ORIGIN      
        1 mgltqcgqfl ltytytmdms gntslykyll hwwaftpnhv arkvaevtlf gnytiyrels
       61 ivisqwpler sklvihglct nwlhlqptdr ayltittvps lenckdclkv aasyeeegee
      121 laanwdgcvr cnclqhgltv httyevispy prfratvcln ywnhvvvntg nflhvlrvdl
      181 dipksknqkt cdkqdtvvtd tvpldmsdve eldytkvver yecsdeklct pecaiqspii
      241 ehdyleepik lndrlnrdsl ntsiinqsdc vcdinksadp ttvkscecmd vseckcrtas
      301 pisrteqqai svrdkilqdf cedmsqelni gsdlitlvkh pscsprstpq rlpsdlkamt
      361 twsspiltpp sdilktrnse ssqksirtfq rstspqpgas ednnltsvns plhsvsvsps
      421 sssrlmsppv trsfrhpspr krsslhsppp ivnstqsrtr pthklileae kayeftdeaq
      481 etceklssfr krrladkkye fcdetedaen ivpfkhirdq fkhrgcsihq ipssptmspa
      541 ltngkkhwvd sdqsetddln msqdqngvnd lttvdstekn vlrplnqnql pngcisrers
      601 gkhcfnsspl vpkinlqypt ikctahfkrs yielddemis vitdvedeet ggyvsyqcvl
      661 pmhvhgsgyv qmqmisnska eklivpcvsi nqlsfdietf shhiadwicm rfkkrywhcs
      721 dydieiidvc alsgdiicll imkiqasein nqtqcsqerk qyevgckftw nidtsqyrit
      781 dvlpmeevkp eswkpncidv pnfrtplwnp trrlapklre kiqqpyahtv rflhnemtlm
      841 gesitrlydl dnlvefyitp mpnypsie
//
LOCUS       XP_034185446             868 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606727 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185446
VERSION     XP_034185446.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329555.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..868
                     /product="uncharacterized protein LOC117606727 isoform X2"
                     /calculated_mol_wt=99123
     Region          1..181
                     /region_name="DCAF15_WD40"
                     /note="DDB1-and CUL4-substrate receptor 15, WD repeat;
                     pfam14939"
                     /db_xref="CDD:464387"
     Site            order(60,78,80..81,89,150,152..153,155)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411024"
     Site            152..157
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411024"
     Region          <205..>454
                     /region_name="PLN03188"
                     /note="kinesin-12 family protein; Provisional"
                     /db_xref="CDD:215621"
     Region          <363..469
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          624..857
                     /region_name="DCAF15-CTD"
                     /note="C-terminal domain of DDB1- and CUL4-associated
                     factor 15; cd20913"
                     /db_xref="CDD:411023"
     Site            order(725,812,815..816,819)
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411023"
     Site            order(728,730,737,767,778..782,784,821..822,828..829)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411023"
     Site            order(805..806,808..809,812..813,816..817,820,823,832,
                     851..852)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411023"
     CDS             1..868
                     /gene="LOC117606727"
                     /coded_by="XM_034329555.2:265..2871"
                     /db_xref="GeneID:117606727"
ORIGIN      
        1 mgltqcgqfl ltytytmdms gntslykyll hwwaftpnhv arkvaevtlf gnytiyrels
       61 ivisqwpler sklvihglct nwlhlqptdr ayltittvps lenckdclkv aasyeeegee
      121 laanwdgcvr cnclqhgltv httyevispy prfratvcln ywnhvvvntg nflhvlrvdl
      181 dipksknqkt cdkqdtvvtd tvpldmsdve eldytkvver yecsdeklct pecaiqspii
      241 ehdyleepik lndrlnrdsl ntsiinqsdc vcdinksadp ttvkscecmd vseckcrtas
      301 pisrteqqai svrdkilqdf cedmsqelni gsdlitlvkh pscsprstpq rlpsdlkamt
      361 twsspiltpp sdilktrnse ssqksirtfq rstspqpgas ednnltsvns plhsvsvsps
      421 sssrlmsppv trsfrhpspr krsslhsppp ivnstqsrtr pthklileae kayeftdeaq
      481 etceklssfr krrladkkye fcdetedaen ivpfkhirdq fkhrgcsihq ipssptmspa
      541 ltngkkhwvd sdqsetddln msqdqngvnd lttvdstekn vlrplnqnql pngcisrers
      601 gkhcfnsspl vpkinlqypt ikctahfkrs yielddemis vitdvedeet ggyvsyqcvl
      661 pmhvhgsgyv qmqmisnska eklivpcvsi nqlsfdietf shhiadwicm rfkkrywhcs
      721 dydieiidvc alsgdiicll imkiqasein nqtqcsqerk qyevgckftw nidtsqyrit
      781 dvlpmeevkp eswkpncidv pnfrtplwnp trrlapklre kiqqpyahtv rflhnemtlm
      841 gesitrlydl dnlvefyitp mpnypsie
//
LOCUS       XP_076545886             987 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606727 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545886
VERSION     XP_076545886.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689771.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..987
                     /product="uncharacterized protein LOC117606727 isoform X3"
                     /calculated_mol_wt=112704
     Site            order(45..48,50..51,53..55,68..71,75,77..78,102..103,105,
                     131,133)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411024"
     Region          65..278
                     /region_name="DCAF15_WD40"
                     /note="DDB1-and CUL4-substrate receptor 15, WD repeat;
                     pfam14939"
                     /db_xref="CDD:464387"
     Site            order(157,175,177..178,186,247,249..250,252)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:411024"
     Site            249..254
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411024"
     Region          <302..>551
                     /region_name="PLN03188"
                     /note="kinesin-12 family protein; Provisional"
                     /db_xref="CDD:215621"
     Region          <460..566
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     Region          721..933
                     /region_name="DCAF15-CTD"
                     /note="C-terminal domain of DDB1- and CUL4-associated
                     factor 15; cd20913"
                     /db_xref="CDD:411023"
     Site            order(822,909,912..913,916)
                     /site_type="other"
                     /note="aryl sulfonamide binding site [chemical binding]"
                     /db_xref="CDD:411023"
     Site            order(825,827,834,864,875..879,881,918..919,925..926)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411023"
     CDS             1..987
                     /gene="LOC117606727"
                     /coded_by="XM_076689771.1:7..2970"
                     /db_xref="GeneID:117606727"
ORIGIN      
        1 madasssdtt sdccnewfsd itsekseyii vgqkpclshr rskrhflqkl flreirgcls
       61 ahnyaseerl fatvpswrhl pllhvipksa leaghivmgl tqcgqfllty tytmdmsgnt
      121 slykyllhww aftpnhvark vaevtlfgny tiyrelsivi sqwplerskl vihglctnwl
      181 hlqptdrayl tittvpslen ckdclkvaas yeeegeelaa nwdgcvrcnc lqhgltvhtt
      241 yevispyprf ratvclnywn hvvvntgnfl hvlrvdldip ksknqktcdk qdtvvtdtvp
      301 ldmsdveeld ytkvveryec sdeklctpec aiqspiiehd yleepiklnd rlnrdslnts
      361 iinqsdcvcd inksadpttv kscecmdvse ckcrtaspis rteqqaisvr dkilqdfced
      421 msqelnigsd litlvkhpsc sprstpqrlp sdlkamttws spiltppsdi lktrnsessq
      481 ksirtfqrst spqpgasedn nltsvnsplh svsvspssss rlmsppvtrs frhpsprkrs
      541 slhspppivn stqsrtrpth klileaekay eftdeaqetc eklssfrkrr ladkkyefcd
      601 etedaenivp fkhirdqfkh rgcsihqips sptmspaltn gkkhwvdsdq setddlnmsq
      661 dqngvndltt vdsteknvlr plnqnqlpng cisrersgkh cfnssplvpk inlqyptikc
      721 tahfkrsyie lddemisvit dvedeetggy vsyqcvlpmh vhgsgyvqmq misnskaekl
      781 ivpcvsinql sfdietfshh iadwicmrfk krywhcsdyd ieiidvcals gdiicllimk
      841 iqaseinnqt qcsqerkqye vgckftwnid tsqyritdvl pmeevkpesw kpncidvpnf
      901 rtplwnptrr lapklrekiq qpyahtvrfl hnemtlmgec feenvnlilt ritysiifsg
      961 esitrlydld nlvefyitpm pnypsie
//
LOCUS       XP_034185447             892 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit C [Osmia
            lignaria lignaria].
ACCESSION   XP_034185447
VERSION     XP_034185447.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329556.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185447.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..892
                     /product="eukaryotic translation initiation factor 3
                     subunit C"
                     /calculated_mol_wt=104077
     Region          29..675
                     /region_name="eIF-3c_N"
                     /note="Eukaryotic translation initiation factor 3 subunit
                     8 N-terminus; pfam05470"
                     /db_xref="CDD:428482"
     Region          748..826
                     /region_name="PINT"
                     /note="motif in proteasome subunits, Int-6, Nip-1 and
                     TRIP-15; smart00088"
                     /db_xref="CDD:214509"
     CDS             1..892
                     /gene="eIF3c"
                     /coded_by="XM_034329556.2:98..2776"
                     /db_xref="GeneID:117606728"
ORIGIN      
        1 msrffatgsd sesdtaseee qiqktptaaf tfsdeeeetk rvvrstkekr yeeianlikl
       61 irnykkikdm ssmlssfedl mrayqkalpv iakeeggqtp rfyirclvem edfinevwed
      121 rdgrknmskn nsksltslrq klrkynkdfe edivrfrenp dqdddeeeek peevvqseee
      181 ddsavafkka gseasepdis kfkksvvdge dasesesdwg sssdsestss edegqyqnir
      241 erfikraadk edeedkvkrk eqrkerkeke rkkkkdeddg egewetvkgg vaipsekpkm
      301 fakdaeinva avlkklseii aargkkrtdr reqiellhel qsiadahnlg pavavkikfs
      361 ivssifdynp kvsdamkpey wskilerite mldmllstkd miiaevisee geeyetppyk
      421 ihgcvltlve rldeefikll kecdphsney verlkdekqv saiidkvqey lerqgtvsel
      481 crvylrkieh lyykfdpnvl kqkegelpad ivtsvqvmek lckyvyahdg tdrlrtrail
      541 shiyhhalhd nwfqardlil mshlqetiqh sdpatqilyn rtiahlglca frhanikdah
      601 nclvdlmvtg kvkellaqgl lpqrqhersk eqekiekqrq mpfhmhinle llecvylvsa
      661 mlieipymaa hefdarrrmi sktfyqqlrs serqslvgpp esmrehvvaa akamrngnws
      721 acnnfiinek mnakvwdlfy qadkvrdmlt rlikeealrt ylftyshvyd sismpklaem
      781 fqlkrpvvhs iiskmiinee lmaslddpte tvvmhrseps rlqslalqlt dkvnnfvdsn
      841 erifemkqgn ffsrgnqgnf rdrqnynrqg qdwgrqrrdr drdrnreenr ny
//
LOCUS       XP_034185448             881 aa            linear   INV 03-SEP-2025
DEFINITION  regulatory transcription factor Rfx isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185448
VERSION     XP_034185448.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329557.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="regulatory transcription factor Rfx isoform X1"
                     /calculated_mol_wt=95369
     Region          <65..>288
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          386..463
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     CDS             1..881
                     /gene="Rfx"
                     /coded_by="XM_034329557.2:187..2832"
                     /db_xref="GeneID:117606729"
ORIGIN      
        1 mdwpeddeyp svfskrysim maeesslpii yyrsqnslsn sslhkrggnn ggdtvgverg
       61 egviamtttg aqyalaastr annsggssts vgvepkpsvv vvttssssgs gsgsvaqsqs
      121 argqqlitvv tspdpsspnl qpiqllvqdp letaadssng stgtpahvta qvvvntthsg
      181 qhtlvdsdaa stsagtivgg sphfitvtgt sdspsyaslt tavvsgepea vgsesvshpt
      241 yvqyvegdgs tyiptngqmt ypvyavgeag amytpassqy ytpastpvty aqvtgqgsns
      301 ttgqllsqgn gtyliqqsvv dgdptthali saaaaraspq tenaesvvsg gggaylisgn
      361 sggatvtved aaaaaanmth atrvsqatvh wllenyetad gvslprstly nhylrhcsen
      421 kldpvnaasf gklirsvflg lrtrrlgtrg nskyhyygir vkpssplvml nedgtprqqq
      481 nansqtkrfk fvnqkqdaty ennthsntni ssntsppqyh qylgeasgai pefpeiivgh
      541 gsslpedctl edidtfrsiy rehceaflda vlnfefatve slwrefwrsq dnnngdecee
      601 ekylsktkly qickcsevqd firkvdytfy qnlvevlmpn vlrpipsslt qsirnfakgl
      661 eswltsamad cpeemtqikl tavsafaqtl rrytslnhla qaaravlqns sqinqmladl
      721 nrvdfhnvqe qaswvcqcdy amvqrleadf kvtlqqqnsl edwaiwlksv vtqvlkpfee
      781 kptfakaarq fllkwsfyss mvirdltlrs aasfgsfhli rllydeymfy liehqvavat
      841 gttpiavmgd ksqtcslvsa fgatsngdts ntcqpkrmkl s
//
LOCUS       XP_034185449             877 aa            linear   INV 03-SEP-2025
DEFINITION  regulatory transcription factor Rfx isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185449
VERSION     XP_034185449.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329558.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..877
                     /product="regulatory transcription factor Rfx isoform X2"
                     /calculated_mol_wt=94916
     Region          118..>299
                     /region_name="Chi1"
                     /note="Chitinase [Carbohydrate transport and metabolism];
                     COG3469"
                     /db_xref="CDD:442692"
     Region          382..459
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     CDS             1..877
                     /gene="Rfx"
                     /coded_by="XM_034329558.2:187..2820"
                     /db_xref="GeneID:117606729"
ORIGIN      
        1 mdwpeddeyp svfskrysim maeesslpii yyrsqnslsn sslhkrggnn ggdtvgverg
       61 egviamtttg aqyalaastr annsggssts vgvepkpsvv vvttssssgs gsgsvaqsqs
      121 argqqlitvv tspdpsspnl qpiqdpleta adssngstgt pahvtaqvvv ntthsgqhtl
      181 vdsdaastsa gtivggsphf itvtgtsdsp syaslttavv sgepeavgse svshptyvqy
      241 vegdgstyip tngqmtypvy avgeagamyt passqyytpa stpvtyaqvt gqgsnsttgq
      301 llsqgngtyl iqqsvvdgdp tthalisaaa araspqtena esvvsgggga ylisgnsgga
      361 tvtvedaaaa aanmthatrv sqatvhwlle nyetadgvsl prstlynhyl rhcsenkldp
      421 vnaasfgkli rsvflglrtr rlgtrgnsky hyygirvkps splvmlnedg tprqqqnans
      481 qtkrfkfvnq kqdatyennt hsntnissnt sppqyhqylg easgaipefp eiivghgssl
      541 pedctledid tfrsiyrehc eafldavlnf efatveslwr efwrsqdnnn gdeceeekyl
      601 sktklyqick csevqdfirk vdytfyqnlv evlmpnvlrp ipssltqsir nfakgleswl
      661 tsamadcpee mtqikltavs afaqtlrryt slnhlaqaar avlqnssqin qmladlnrvd
      721 fhnvqeqasw vcqcdyamvq rleadfkvtl qqqnsledwa iwlksvvtqv lkpfeekptf
      781 akaarqfllk wsfyssmvir dltlrsaasf gsfhlirlly deymfylieh qvavatgttp
      841 iavmgdksqt cslvsafgat sngdtsntcq pkrmkls
//
LOCUS       XP_034185450             866 aa            linear   INV 03-SEP-2025
DEFINITION  regulatory transcription factor Rfx isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185450
VERSION     XP_034185450.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329559.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..866
                     /product="regulatory transcription factor Rfx isoform X3"
                     /calculated_mol_wt=93931
     Region          152..313
                     /region_name="RFX1_trans_act"
                     /note="RFX1 transcription activation region; pfam04589"
                     /db_xref="CDD:461362"
     Region          371..448
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     CDS             1..866
                     /gene="Rfx"
                     /coded_by="XM_034329559.2:185..2785"
                     /db_xref="GeneID:117606729"
ORIGIN      
        1 mdwpeddeyp svfskrysim maeesslpii yyrsqnslsn sslhkrggnn ggdtvgverg
       61 egviamtttg aqyalaastr annsggssts vgvepkpsvv vvttssssgs gsgsvaqsqs
      121 argqqlitvv tspdpsspnl qpiqllvqdp letaadssng stgtpahvta qvvvntthsg
      181 qhtlvdsdaa stsagtivgg sphfitvtvs gepeavgses vshptyvqyv egdgstyipt
      241 ngqmtypvya vgeagamytp assqyytpas tpvtyaqvtg qgsnsttgql lsqgngtyli
      301 qqsvvdgdpt thalisaaaa raspqtenae svvsggggay lisgnsggat vtvedaaaaa
      361 anmthatrvs qatvhwllen yetadgvslp rstlynhylr hcsenkldpv naasfgklir
      421 svflglrtrr lgtrgnskyh yygirvkpss plvmlnedgt prqqqnansq tkrfkfvnqk
      481 qdatyennth sntnissnts ppqyhqylge asgaipefpe iivghgsslp edctledidt
      541 frsiyrehce afldavlnfe fatveslwre fwrsqdnnng deceeekyls ktklyqickc
      601 sevqdfirkv dytfyqnlve vlmpnvlrpi pssltqsirn fakgleswlt samadcpeem
      661 tqikltavsa faqtlrryts lnhlaqaara vlqnssqinq mladlnrvdf hnvqeqaswv
      721 cqcdyamvqr leadfkvtlq qqnsledwai wlksvvtqvl kpfeekptfa kaarqfllkw
      781 sfyssmvird ltlrsaasfg sfhlirllyd eymfyliehq vavatgttpi avmgdksqtc
      841 slvsafgats ngdtsntcqp krmkls
//
LOCUS       XP_076545887             845 aa            linear   INV 03-SEP-2025
DEFINITION  regulatory transcription factor Rfx isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545887
VERSION     XP_076545887.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689772.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..845
                     /product="regulatory transcription factor Rfx isoform X4"
                     /calculated_mol_wt=91083
     Region          <29..>252
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          350..427
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     CDS             1..845
                     /gene="Rfx"
                     /coded_by="XM_076689772.1:668..3205"
                     /db_xref="GeneID:117606729"
ORIGIN      
        1 mktdsslhkr ggnnggdtvg vergegviam tttgaqyala astrannsgg sstsvgvepk
       61 psvvvvttss ssgsgsgsva qsqsargqql itvvtspdps spnlqpiqll vqdpletaad
      121 ssngstgtpa hvtaqvvvnt thsgqhtlvd sdaastsagt ivggsphfit vtgtsdspsy
      181 aslttavvsg epeavgsesv shptyvqyve gdgstyiptn gqmtypvyav geagamytpa
      241 ssqyytpast pvtyaqvtgq gsnsttgqll sqgngtyliq qsvvdgdptt halisaaaar
      301 aspqtenaes vvsggggayl isgnsggatv tvedaaaaaa nmthatrvsq atvhwlleny
      361 etadgvslpr stlynhylrh csenkldpvn aasfgklirs vflglrtrrl gtrgnskyhy
      421 ygirvkpssp lvmlnedgtp rqqqnansqt krfkfvnqkq datyennths ntnissntsp
      481 pqyhqylgea sgaipefpei ivghgsslpe dctledidtf rsiyrehcea fldavlnfef
      541 atveslwref wrsqdnnngd eceeekylsk tklyqickcs evqdfirkvd ytfyqnlvev
      601 lmpnvlrpip ssltqsirnf akgleswlts amadcpeemt qikltavsaf aqtlrrytsl
      661 nhlaqaarav lqnssqinqm ladlnrvdfh nvqeqaswvc qcdyamvqrl eadfkvtlqq
      721 qnsledwaiw lksvvtqvlk pfeekptfak aarqfllkws fyssmvirdl tlrsaasfgs
      781 fhlirllyde ymfyliehqv avatgttpia vmgdksqtcs lvsafgatsn gdtsntcqpk
      841 rmkls
//
LOCUS       XP_034185455             837 aa            linear   INV 03-SEP-2025
DEFINITION  regulatory transcription factor Rfx isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185455
VERSION     XP_034185455.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329564.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..837
                     /product="regulatory transcription factor Rfx isoform X5"
                     /calculated_mol_wt=91279
     Region          <65..>288
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          344..419
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     CDS             1..837
                     /gene="Rfx"
                     /coded_by="XM_034329564.2:187..2700"
                     /db_xref="GeneID:117606729"
ORIGIN      
        1 mdwpeddeyp svfskrysim maeesslpii yyrsqnslsn sslhkrggnn ggdtvgverg
       61 egviamtttg aqyalaastr annsggssts vgvepkpsvv vvttssssgs gsgsvaqsqs
      121 argqqlitvv tspdpsspnl qpiqllvqdp letaadssng stgtpahvta qvvvntthsg
      181 qhtlvdsdaa stsagtivgg sphfitvtgt sdspsyaslt tavvsgepea vgsesvshpt
      241 yvqyvegdgs tyiptngqmt ypvyavgeag amytpassqy ytpastpvty aqvtgqgsns
      301 ttgqllsqgn gtyliqqsvv dgdptthali saaaaraspq tenavhwlle nyetadgvsl
      361 prstlynhyl rhcsenkldp vnaasfgkli rsvflglrtr rlgtrgnsky hyygirvkps
      421 splvmlnedg tprqqqnans qtkrfkfvnq kqdatyennt hsntnissnt sppqyhqylg
      481 easgaipefp eiivghgssl pedctledid tfrsiyrehc eafldavlnf efatveslwr
      541 efwrsqdnnn gdeceeekyl sktklyqick csevqdfirk vdytfyqnlv evlmpnvlrp
      601 ipssltqsir nfakgleswl tsamadcpee mtqikltavs afaqtlrryt slnhlaqaar
      661 avlqnssqin qmladlnrvd fhnvqeqasw vcqcdyamvq rleadfkvtl qqqnsledwa
      721 iwlksvvtqv lkpfeekptf akaarqfllk wsfyssmvir dltlrsaasf gsfhlirlly
      781 deymfylieh qvavatgttp iavmgdksqt cslvsafgat sngdtsntcq pkrmkls
//
LOCUS       XP_034185456             816 aa            linear   INV 03-SEP-2025
DEFINITION  regulatory transcription factor Rfx isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185456
VERSION     XP_034185456.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329565.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..816
                     /product="regulatory transcription factor Rfx isoform X6"
                     /calculated_mol_wt=88158
     Region          <11..>223
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          321..398
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     CDS             1..816
                     /gene="Rfx"
                     /coded_by="XM_034329565.2:205..2655"
                     /db_xref="GeneID:117606729"
ORIGIN      
        1 mtttgaqyal aastrannsg gsstsvgvep kpsvvvvtts sssgsgsgsv aqsqsargqq
       61 litvvtspdp sspnlqpiql lvqdpletaa dssngstgtp ahvtaqvvvn tthsgqhtlv
      121 dsdaastsag tivggsphfi tvtgtsdsps yaslttavvs gepeavgses vshptyvqyv
      181 egdgstyipt ngqmtypvya vgeagamytp assqyytpas tpvtyaqvtg qgsnsttgql
      241 lsqgngtyli qqsvvdgdpt thalisaaaa raspqtenae svvsggggay lisgnsggat
      301 vtvedaaaaa anmthatrvs qatvhwllen yetadgvslp rstlynhylr hcsenkldpv
      361 naasfgklir svflglrtrr lgtrgnskyh yygirvkpss plvmlnedgt prqqqnansq
      421 tkrfkfvnqk qdatyennth sntnissnts ppqyhqylge asgaipefpe iivghgsslp
      481 edctledidt frsiyrehce afldavlnfe fatveslwre fwrsqdnnng deceeekyls
      541 ktklyqickc sevqdfirkv dytfyqnlve vlmpnvlrpi pssltqsirn fakgleswlt
      601 samadcpeem tqikltavsa faqtlrryts lnhlaqaara vlqnssqinq mladlnrvdf
      661 hnvqeqaswv cqcdyamvqr leadfkvtlq qqnsledwai wlksvvtqvl kpfeekptfa
      721 kaarqfllkw sfyssmvird ltlrsaasfg sfhlirllyd eymfyliehq vavatgttpi
      781 avmgdksqtc slvsafgats ngdtsntcqp krmkls
//
LOCUS       XP_076545888             859 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related protein 9 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545888
VERSION     XP_076545888.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689773.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..859
                     /product="autophagy-related protein 9 isoform X1"
                     /calculated_mol_wt=98141
     Region          67..559
                     /region_name="ATG9"
                     /note="Autophagy protein ATG9; pfam04109"
                     /db_xref="CDD:461177"
     CDS             1..859
                     /gene="Atg9"
                     /coded_by="XM_076689773.1:637..3216"
                     /db_xref="GeneID:117606480"
ORIGIN      
        1 mkretvitcv esnrraktmt tvldgsyqrm epyegeeeed gedddeheet pqesgvmihv
       61 vpegnkarwn hvedldsfft rmyhyhqkhg facmmlqeaf elgqfifvva fstflfhcid
      121 ysvlfknkvt nstshktsis dailstgect asvglvtwic ilvagffwil raikvlyhft
      181 qfwdiklffn talkiddsdl dnltwheiqk rvrevqkeqe mcihkrelte ldiyhrilrf
      241 knymiamink sllpvrlkvp iigevifmtr glkynmelll fsdlsypfyi ggpwspfenn
      301 whlkedykkl nkrqelarsl skhilwvgia nfmlcplill wqilysffny geiikrepgt
      361 lgtrmwslyg rlylrhfnel dhelnarlnr ayhpaskyms iftspimtvl aknvafvags
      421 vlavlliltv ydedvltveh vlttitilga lvagarafip denlvwcpet lltavlahth
      481 yrpdswrgha htqttraqva qlfqyravhl leelispllt pfilcfhmrq raldivdfyr
      541 nftievtgvg dvcsfaqmdv rkhgnpmwqt vtqspiedra attgydnqry gmepeklqip
      601 msnqytqaen gktelslihf tltnpewkpp shaenfvtal rerakkevhg vhgeinplla
      661 slsslsglgp gyndivsnii rstmvnqvlg gastsnvfan qpatstaftg gvhessifrs
      721 dflsyaavrr glskaegpih ndkgflyglq qgmsnqslga svfgsthemp tdfsvpvelt
      781 aadmslstly lhelhhrqvq rrgyqematr siwqqspvqe latlpkvrqe raplllhqds
      841 sirsnrdsay tanthldni
//
LOCUS       XP_076545889             859 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related protein 9 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545889
VERSION     XP_076545889.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689774.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..859
                     /product="autophagy-related protein 9 isoform X1"
                     /calculated_mol_wt=98141
     Region          67..559
                     /region_name="ATG9"
                     /note="Autophagy protein ATG9; pfam04109"
                     /db_xref="CDD:461177"
     CDS             1..859
                     /gene="Atg9"
                     /coded_by="XM_076689774.1:550..3129"
                     /db_xref="GeneID:117606480"
ORIGIN      
        1 mkretvitcv esnrraktmt tvldgsyqrm epyegeeeed gedddeheet pqesgvmihv
       61 vpegnkarwn hvedldsfft rmyhyhqkhg facmmlqeaf elgqfifvva fstflfhcid
      121 ysvlfknkvt nstshktsis dailstgect asvglvtwic ilvagffwil raikvlyhft
      181 qfwdiklffn talkiddsdl dnltwheiqk rvrevqkeqe mcihkrelte ldiyhrilrf
      241 knymiamink sllpvrlkvp iigevifmtr glkynmelll fsdlsypfyi ggpwspfenn
      301 whlkedykkl nkrqelarsl skhilwvgia nfmlcplill wqilysffny geiikrepgt
      361 lgtrmwslyg rlylrhfnel dhelnarlnr ayhpaskyms iftspimtvl aknvafvags
      421 vlavlliltv ydedvltveh vlttitilga lvagarafip denlvwcpet lltavlahth
      481 yrpdswrgha htqttraqva qlfqyravhl leelispllt pfilcfhmrq raldivdfyr
      541 nftievtgvg dvcsfaqmdv rkhgnpmwqt vtqspiedra attgydnqry gmepeklqip
      601 msnqytqaen gktelslihf tltnpewkpp shaenfvtal rerakkevhg vhgeinplla
      661 slsslsglgp gyndivsnii rstmvnqvlg gastsnvfan qpatstaftg gvhessifrs
      721 dflsyaavrr glskaegpih ndkgflyglq qgmsnqslga svfgsthemp tdfsvpvelt
      781 aadmslstly lhelhhrqvq rrgyqematr siwqqspvqe latlpkvrqe raplllhqds
      841 sirsnrdsay tanthldni
//
LOCUS       XP_076545890             859 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related protein 9 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545890
VERSION     XP_076545890.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689775.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..859
                     /product="autophagy-related protein 9 isoform X1"
                     /calculated_mol_wt=98141
     Region          67..559
                     /region_name="ATG9"
                     /note="Autophagy protein ATG9; pfam04109"
                     /db_xref="CDD:461177"
     CDS             1..859
                     /gene="Atg9"
                     /coded_by="XM_076689775.1:197..2776"
                     /db_xref="GeneID:117606480"
ORIGIN      
        1 mkretvitcv esnrraktmt tvldgsyqrm epyegeeeed gedddeheet pqesgvmihv
       61 vpegnkarwn hvedldsfft rmyhyhqkhg facmmlqeaf elgqfifvva fstflfhcid
      121 ysvlfknkvt nstshktsis dailstgect asvglvtwic ilvagffwil raikvlyhft
      181 qfwdiklffn talkiddsdl dnltwheiqk rvrevqkeqe mcihkrelte ldiyhrilrf
      241 knymiamink sllpvrlkvp iigevifmtr glkynmelll fsdlsypfyi ggpwspfenn
      301 whlkedykkl nkrqelarsl skhilwvgia nfmlcplill wqilysffny geiikrepgt
      361 lgtrmwslyg rlylrhfnel dhelnarlnr ayhpaskyms iftspimtvl aknvafvags
      421 vlavlliltv ydedvltveh vlttitilga lvagarafip denlvwcpet lltavlahth
      481 yrpdswrgha htqttraqva qlfqyravhl leelispllt pfilcfhmrq raldivdfyr
      541 nftievtgvg dvcsfaqmdv rkhgnpmwqt vtqspiedra attgydnqry gmepeklqip
      601 msnqytqaen gktelslihf tltnpewkpp shaenfvtal rerakkevhg vhgeinplla
      661 slsslsglgp gyndivsnii rstmvnqvlg gastsnvfan qpatstaftg gvhessifrs
      721 dflsyaavrr glskaegpih ndkgflyglq qgmsnqslga svfgsthemp tdfsvpvelt
      781 aadmslstly lhelhhrqvq rrgyqematr siwqqspvqe latlpkvrqe raplllhqds
      841 sirsnrdsay tanthldni
//
LOCUS       XP_034184864             850 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related protein 9 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184864
VERSION     XP_034184864.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328973.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..850
                     /product="autophagy-related protein 9 isoform X2"
                     /calculated_mol_wt=97184
     Region          67..550
                     /region_name="ATG9"
                     /note="Autophagy protein ATG9; pfam04109"
                     /db_xref="CDD:461177"
     CDS             1..850
                     /gene="Atg9"
                     /coded_by="XM_034328973.2:637..3189"
                     /db_xref="GeneID:117606480"
ORIGIN      
        1 mkretvitcv esnrraktmt tvldgsyqrm epyegeeeed gedddeheet pqesgvmihv
       61 vpegnkarwn hvedldsfft rmyhyhqkhg facmmlqeaf elgqfifvva fstflfhcid
      121 ysvlfknkvt nstshktsis dailstgect asvglvtwic ilvagffwil raikvlyhft
      181 qfwdiklffn talkiddsdl dnltwheiqk rvrevqkeqe mcihkrelte ldiyhrilrf
      241 knymiamink sllpvrlkvp iigevifmtr glkynmelll fwgpwspfen nwhlkedykk
      301 lnkrqelars lskhilwvgi anfmlcplil lwqilysffn ygeiikrepg tlgtrmwsly
      361 grlylrhfne ldhelnarln rayhpaskym siftspimtv laknvafvag svlavllilt
      421 vydedvltve hvlttitilg alvagarafi pdenlvwcpe tlltavlaht hyrpdswrgh
      481 ahtqttraqv aqlfqyravh lleelispll tpfilcfhmr qraldivdfy rnftievtgv
      541 gdvcsfaqmd vrkhgnpmwq tvtqspiedr aattgydnqr ygmepeklqi pmsnqytqae
      601 ngktelslih ftltnpewkp pshaenfvta lrerakkevh gvhgeinpll aslsslsglg
      661 pgyndivsni irstmvnqvl ggastsnvfa nqpatstaft ggvhessifr sdflsyaavr
      721 rglskaegpi hndkgflygl qqgmsnqslg asvfgsthem ptdfsvpvel taadmslstl
      781 ylhelhhrqv qrrgyqemat rsiwqqspvq elatlpkvrq eraplllhqd ssirsnrdsa
      841 ytanthldni
//
LOCUS       XP_076545891             841 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related protein 9 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545891
VERSION     XP_076545891.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689776.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..841
                     /product="autophagy-related protein 9 isoform X3"
                     /calculated_mol_wt=96036
     Region          49..541
                     /region_name="ATG9"
                     /note="Autophagy protein ATG9; pfam04109"
                     /db_xref="CDD:461177"
     CDS             1..841
                     /gene="Atg9"
                     /coded_by="XM_076689776.1:144..2669"
                     /db_xref="GeneID:117606480"
ORIGIN      
        1 mttvldgsyq rmepyegeee edgedddehe etpqesgvmi hvvpegnkar wnhvedldsf
       61 ftrmyhyhqk hgfacmmlqe afelgqfifv vafstflfhc idysvlfknk vtnstshkts
      121 isdailstge ctasvglvtw icilvagffw ilraikvlyh ftqfwdiklf fntalkidds
      181 dldnltwhei qkrvrevqke qemcihkrel teldiyhril rfknymiami nksllpvrlk
      241 vpiigevifm trglkynmel llfsdlsypf yiggpwspfe nnwhlkedyk klnkrqelar
      301 slskhilwvg ianfmlcpli llwqilysff nygeiikrep gtlgtrmwsl ygrlylrhfn
      361 eldhelnarl nrayhpasky msiftspimt vlaknvafva gsvlavllil tvydedvltv
      421 ehvlttitil galvagaraf ipdenlvwcp etlltavlah thyrpdswrg hahtqttraq
      481 vaqlfqyrav hlleelispl ltpfilcfhm rqraldivdf yrnftievtg vgdvcsfaqm
      541 dvrkhgnpmw qtvtqspied raattgydnq rygmepeklq ipmsnqytqa engktelsli
      601 hftltnpewk ppshaenfvt alrerakkev hgvhgeinpl laslsslsgl gpgyndivsn
      661 iirstmvnqv lggastsnvf anqpatstaf tggvhessif rsdflsyaav rrglskaegp
      721 ihndkgflyg lqqgmsnqsl gasvfgsthe mptdfsvpve ltaadmslst lylhelhhrq
      781 vqrrgyqema trsiwqqspv qelatlpkvr qeraplllhq dssirsnrds aytanthldn
      841 i
//
LOCUS       XP_076545892             841 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related protein 9 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545892
VERSION     XP_076545892.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689777.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..841
                     /product="autophagy-related protein 9 isoform X3"
                     /calculated_mol_wt=96036
     Region          49..541
                     /region_name="ATG9"
                     /note="Autophagy protein ATG9; pfam04109"
                     /db_xref="CDD:461177"
     CDS             1..841
                     /gene="Atg9"
                     /coded_by="XM_076689777.1:582..3107"
                     /db_xref="GeneID:117606480"
ORIGIN      
        1 mttvldgsyq rmepyegeee edgedddehe etpqesgvmi hvvpegnkar wnhvedldsf
       61 ftrmyhyhqk hgfacmmlqe afelgqfifv vafstflfhc idysvlfknk vtnstshkts
      121 isdailstge ctasvglvtw icilvagffw ilraikvlyh ftqfwdiklf fntalkidds
      181 dldnltwhei qkrvrevqke qemcihkrel teldiyhril rfknymiami nksllpvrlk
      241 vpiigevifm trglkynmel llfsdlsypf yiggpwspfe nnwhlkedyk klnkrqelar
      301 slskhilwvg ianfmlcpli llwqilysff nygeiikrep gtlgtrmwsl ygrlylrhfn
      361 eldhelnarl nrayhpasky msiftspimt vlaknvafva gsvlavllil tvydedvltv
      421 ehvlttitil galvagaraf ipdenlvwcp etlltavlah thyrpdswrg hahtqttraq
      481 vaqlfqyrav hlleelispl ltpfilcfhm rqraldivdf yrnftievtg vgdvcsfaqm
      541 dvrkhgnpmw qtvtqspied raattgydnq rygmepeklq ipmsnqytqa engktelsli
      601 hftltnpewk ppshaenfvt alrerakkev hgvhgeinpl laslsslsgl gpgyndivsn
      661 iirstmvnqv lggastsnvf anqpatstaf tggvhessif rsdflsyaav rrglskaegp
      721 ihndkgflyg lqqgmsnqsl gasvfgsthe mptdfsvpve ltaadmslst lylhelhhrq
      781 vqrrgyqema trsiwqqspv qelatlpkvr qeraplllhq dssirsnrds aytanthldn
      841 i
//
LOCUS       XP_076545893             841 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related protein 9 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545893
VERSION     XP_076545893.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689778.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..841
                     /product="autophagy-related protein 9 isoform X3"
                     /calculated_mol_wt=96036
     Region          49..541
                     /region_name="ATG9"
                     /note="Autophagy protein ATG9; pfam04109"
                     /db_xref="CDD:461177"
     CDS             1..841
                     /gene="Atg9"
                     /coded_by="XM_076689778.1:138..2663"
                     /db_xref="GeneID:117606480"
ORIGIN      
        1 mttvldgsyq rmepyegeee edgedddehe etpqesgvmi hvvpegnkar wnhvedldsf
       61 ftrmyhyhqk hgfacmmlqe afelgqfifv vafstflfhc idysvlfknk vtnstshkts
      121 isdailstge ctasvglvtw icilvagffw ilraikvlyh ftqfwdiklf fntalkidds
      181 dldnltwhei qkrvrevqke qemcihkrel teldiyhril rfknymiami nksllpvrlk
      241 vpiigevifm trglkynmel llfsdlsypf yiggpwspfe nnwhlkedyk klnkrqelar
      301 slskhilwvg ianfmlcpli llwqilysff nygeiikrep gtlgtrmwsl ygrlylrhfn
      361 eldhelnarl nrayhpasky msiftspimt vlaknvafva gsvlavllil tvydedvltv
      421 ehvlttitil galvagaraf ipdenlvwcp etlltavlah thyrpdswrg hahtqttraq
      481 vaqlfqyrav hlleelispl ltpfilcfhm rqraldivdf yrnftievtg vgdvcsfaqm
      541 dvrkhgnpmw qtvtqspied raattgydnq rygmepeklq ipmsnqytqa engktelsli
      601 hftltnpewk ppshaenfvt alrerakkev hgvhgeinpl laslsslsgl gpgyndivsn
      661 iirstmvnqv lggastsnvf anqpatstaf tggvhessif rsdflsyaav rrglskaegp
      721 ihndkgflyg lqqgmsnqsl gasvfgsthe mptdfsvpve ltaadmslst lylhelhhrq
      781 vqrrgyqema trsiwqqspv qelatlpkvr qeraplllhq dssirsnrds aytanthldn
      841 i
//
LOCUS       XP_034184674             723 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606403 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184674
VERSION     XP_034184674.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328783.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184674.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..723
                     /product="uncharacterized protein LOC117606403 isoform X1"
                     /calculated_mol_wt=74630
     Region          494..538
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(495,498,516,519)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(504..505,507,516..526)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     Region          551..601
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(552,555,573,576)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(561..562,564,573..583,599,601)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     CDS             1..723
                     /gene="LOC117606403"
                     /coded_by="XM_034328783.2:812..2983"
                     /db_xref="GeneID:117606403"
ORIGIN      
        1 mdmssessaa rwyepprstl eppsggagva gvvgnptdyr gyyphagpta hhpaaaaahy
       61 ahtrmsssgv tsgpvsqvcr phfhssvlhp wlsgsgadts ktpwgfptst gstggtvndd
      121 kpqsplgslp ptsgslsshg gagaghhlfs fpptppkdat pdsitastts stnnnnsssa
      181 nnnnnngsnp lsglggggas seyqaavaha avmgafmhhq dalggsgass cdvkpsvmlg
      241 hhhhgapspp hqqhngttng gnggngtngg aggtgqvkqr egnnqsgnvs gsqqasaaqq
      301 qqqqqqqqqq qqqqqqqqqq qqqqqqqeaa yqgnnvaggg gggggassps cresyaaaaa
      361 aaaaaaaaaa aasnthhats gsqydpatsa ynmyqhlqyp stthhhhhhh lhgassssss
      421 sashnphngt avsgifghha aagagnatgv agvnvdskll vghthgntps spsaqqqqqq
      481 kprnksrtsa egrecvncga tstplwrrdg tghylcnacg lyykmngqnr plikpkrrls
      541 lqsaarragt scancktatt tlwrrnqage pvcnacglyy klhnvnrplt mkkegiqtrn
      601 rklsskskkk kaggclglgg vmgdmikasg hldldnkpfh sgfgspmsts qhhhtmhpam
      661 qhymyhtgvg vagglhqgfa ppapppihph shshshshhm tslqglqlaa ttnamtgwrs
      721 eyt
//
LOCUS       XP_034184675             723 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606403 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184675
VERSION     XP_034184675.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328784.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184675.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..723
                     /product="uncharacterized protein LOC117606403 isoform X1"
                     /calculated_mol_wt=74630
     Region          494..538
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(495,498,516,519)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(504..505,507,516..526)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     Region          551..601
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(552,555,573,576)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(561..562,564,573..583,599,601)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     CDS             1..723
                     /gene="LOC117606403"
                     /coded_by="XM_034328784.2:1076..3247"
                     /db_xref="GeneID:117606403"
ORIGIN      
        1 mdmssessaa rwyepprstl eppsggagva gvvgnptdyr gyyphagpta hhpaaaaahy
       61 ahtrmsssgv tsgpvsqvcr phfhssvlhp wlsgsgadts ktpwgfptst gstggtvndd
      121 kpqsplgslp ptsgslsshg gagaghhlfs fpptppkdat pdsitastts stnnnnsssa
      181 nnnnnngsnp lsglggggas seyqaavaha avmgafmhhq dalggsgass cdvkpsvmlg
      241 hhhhgapspp hqqhngttng gnggngtngg aggtgqvkqr egnnqsgnvs gsqqasaaqq
      301 qqqqqqqqqq qqqqqqqqqq qqqqqqqeaa yqgnnvaggg gggggassps cresyaaaaa
      361 aaaaaaaaaa aasnthhats gsqydpatsa ynmyqhlqyp stthhhhhhh lhgassssss
      421 sashnphngt avsgifghha aagagnatgv agvnvdskll vghthgntps spsaqqqqqq
      481 kprnksrtsa egrecvncga tstplwrrdg tghylcnacg lyykmngqnr plikpkrrls
      541 lqsaarragt scancktatt tlwrrnqage pvcnacglyy klhnvnrplt mkkegiqtrn
      601 rklsskskkk kaggclglgg vmgdmikasg hldldnkpfh sgfgspmsts qhhhtmhpam
      661 qhymyhtgvg vagglhqgfa ppapppihph shshshshhm tslqglqlaa ttnamtgwrs
      721 eyt
//
LOCUS       XP_076545894             723 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606403 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545894
VERSION     XP_076545894.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689779.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..723
                     /product="uncharacterized protein LOC117606403 isoform X1"
                     /calculated_mol_wt=74630
     Region          494..538
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(495,498,516,519)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(504..505,507,516..526)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     Region          551..601
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(552,555,573,576)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(561..562,564,573..583,599,601)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     CDS             1..723
                     /gene="LOC117606403"
                     /coded_by="XM_076689779.1:325..2496"
                     /db_xref="GeneID:117606403"
ORIGIN      
        1 mdmssessaa rwyepprstl eppsggagva gvvgnptdyr gyyphagpta hhpaaaaahy
       61 ahtrmsssgv tsgpvsqvcr phfhssvlhp wlsgsgadts ktpwgfptst gstggtvndd
      121 kpqsplgslp ptsgslsshg gagaghhlfs fpptppkdat pdsitastts stnnnnsssa
      181 nnnnnngsnp lsglggggas seyqaavaha avmgafmhhq dalggsgass cdvkpsvmlg
      241 hhhhgapspp hqqhngttng gnggngtngg aggtgqvkqr egnnqsgnvs gsqqasaaqq
      301 qqqqqqqqqq qqqqqqqqqq qqqqqqqeaa yqgnnvaggg gggggassps cresyaaaaa
      361 aaaaaaaaaa aasnthhats gsqydpatsa ynmyqhlqyp stthhhhhhh lhgassssss
      421 sashnphngt avsgifghha aagagnatgv agvnvdskll vghthgntps spsaqqqqqq
      481 kprnksrtsa egrecvncga tstplwrrdg tghylcnacg lyykmngqnr plikpkrrls
      541 lqsaarragt scancktatt tlwrrnqage pvcnacglyy klhnvnrplt mkkegiqtrn
      601 rklsskskkk kaggclglgg vmgdmikasg hldldnkpfh sgfgspmsts qhhhtmhpam
      661 qhymyhtgvg vagglhqgfa ppapppihph shshshshhm tslqglqlaa ttnamtgwrs
      721 eyt
//
LOCUS       XP_034184676             723 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606403 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184676
VERSION     XP_034184676.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328785.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184676.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..723
                     /product="uncharacterized protein LOC117606403 isoform X1"
                     /calculated_mol_wt=74630
     Region          494..538
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(495,498,516,519)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(504..505,507,516..526)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     Region          551..601
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(552,555,573,576)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(561..562,564,573..583,599,601)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     CDS             1..723
                     /gene="LOC117606403"
                     /coded_by="XM_034328785.2:597..2768"
                     /db_xref="GeneID:117606403"
ORIGIN      
        1 mdmssessaa rwyepprstl eppsggagva gvvgnptdyr gyyphagpta hhpaaaaahy
       61 ahtrmsssgv tsgpvsqvcr phfhssvlhp wlsgsgadts ktpwgfptst gstggtvndd
      121 kpqsplgslp ptsgslsshg gagaghhlfs fpptppkdat pdsitastts stnnnnsssa
      181 nnnnnngsnp lsglggggas seyqaavaha avmgafmhhq dalggsgass cdvkpsvmlg
      241 hhhhgapspp hqqhngttng gnggngtngg aggtgqvkqr egnnqsgnvs gsqqasaaqq
      301 qqqqqqqqqq qqqqqqqqqq qqqqqqqeaa yqgnnvaggg gggggassps cresyaaaaa
      361 aaaaaaaaaa aasnthhats gsqydpatsa ynmyqhlqyp stthhhhhhh lhgassssss
      421 sashnphngt avsgifghha aagagnatgv agvnvdskll vghthgntps spsaqqqqqq
      481 kprnksrtsa egrecvncga tstplwrrdg tghylcnacg lyykmngqnr plikpkrrls
      541 lqsaarragt scancktatt tlwrrnqage pvcnacglyy klhnvnrplt mkkegiqtrn
      601 rklsskskkk kaggclglgg vmgdmikasg hldldnkpfh sgfgspmsts qhhhtmhpam
      661 qhymyhtgvg vagglhqgfa ppapppihph shshshshhm tslqglqlaa ttnamtgwrs
      721 eyt
//
LOCUS       XP_034184677             723 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606403 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184677
VERSION     XP_034184677.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328786.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184677.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..723
                     /product="uncharacterized protein LOC117606403 isoform X1"
                     /calculated_mol_wt=74630
     Region          494..538
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(495,498,516,519)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(504..505,507,516..526)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     Region          551..601
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(552,555,573,576)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(561..562,564,573..583,599,601)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     CDS             1..723
                     /gene="LOC117606403"
                     /coded_by="XM_034328786.2:592..2763"
                     /db_xref="GeneID:117606403"
ORIGIN      
        1 mdmssessaa rwyepprstl eppsggagva gvvgnptdyr gyyphagpta hhpaaaaahy
       61 ahtrmsssgv tsgpvsqvcr phfhssvlhp wlsgsgadts ktpwgfptst gstggtvndd
      121 kpqsplgslp ptsgslsshg gagaghhlfs fpptppkdat pdsitastts stnnnnsssa
      181 nnnnnngsnp lsglggggas seyqaavaha avmgafmhhq dalggsgass cdvkpsvmlg
      241 hhhhgapspp hqqhngttng gnggngtngg aggtgqvkqr egnnqsgnvs gsqqasaaqq
      301 qqqqqqqqqq qqqqqqqqqq qqqqqqqeaa yqgnnvaggg gggggassps cresyaaaaa
      361 aaaaaaaaaa aasnthhats gsqydpatsa ynmyqhlqyp stthhhhhhh lhgassssss
      421 sashnphngt avsgifghha aagagnatgv agvnvdskll vghthgntps spsaqqqqqq
      481 kprnksrtsa egrecvncga tstplwrrdg tghylcnacg lyykmngqnr plikpkrrls
      541 lqsaarragt scancktatt tlwrrnqage pvcnacglyy klhnvnrplt mkkegiqtrn
      601 rklsskskkk kaggclglgg vmgdmikasg hldldnkpfh sgfgspmsts qhhhtmhpam
      661 qhymyhtgvg vagglhqgfa ppapppihph shshshshhm tslqglqlaa ttnamtgwrs
      721 eyt
//
LOCUS       XP_034184678             715 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606403 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184678
VERSION     XP_034184678.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328787.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184678.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..715
                     /product="uncharacterized protein LOC117606403 isoform X2"
                     /calculated_mol_wt=73689
     Region          494..538
                     /region_name="ZnF_GATA"
                     /note="Zinc finger DNA binding domain; binds specifically
                     to DNA consensus sequence [AT]GATA[AG] promoter elements;
                     a subset of family members may also bind protein;
                     zinc-finger consensus topology is C-X(2)-C-X(17)-C-X(2)-C;
                     cd00202"
                     /db_xref="CDD:238123"
     Site            order(495,498,516,519)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238123"
     Site            order(504..505,507,516..526)
                     /site_type="DNA binding"
                     /note="DNA-binding region [nucleotide binding]"
                     /db_xref="CDD:238123"
     Region          547..>585
                     /region_name="ZnF_GATA"
                     /note="zinc finger binding to DNA consensus sequence
                     [AT]GATA[AG]; smart00401"
                     /db_xref="CDD:214648"
     CDS             1..715
                     /gene="LOC117606403"
                     /coded_by="XM_034328787.2:813..2960"
                     /db_xref="GeneID:117606403"
ORIGIN      
        1 mdmssessaa rwyepprstl eppsggagva gvvgnptdyr gyyphagpta hhpaaaaahy
       61 ahtrmsssgv tsgpvsqvcr phfhssvlhp wlsgsgadts ktpwgfptst gstggtvndd
      121 kpqsplgslp ptsgslsshg gagaghhlfs fpptppkdat pdsitastts stnnnnsssa
      181 nnnnnngsnp lsglggggas seyqaavaha avmgafmhhq dalggsgass cdvkpsvmlg
      241 hhhhgapspp hqqhngttng gnggngtngg aggtgqvkqr egnnqsgnvs gsqqasaaqq
      301 qqqqqqqqqq qqqqqqqqqq qqqqqqqeaa yqgnnvaggg gggggassps cresyaaaaa
      361 aaaaaaaaaa aasnthhats gsqydpatsa ynmyqhlqyp stthhhhhhh lhgassssss
      421 sashnphngt avsgifghha aagagnatgv agvnvdskll vghthgntps spsaqqqqqq
      481 kprnksrtsa egrecvncga tstplwrrdg tghylcnacg lyykmngqnr plikpkrrls
      541 lqsaarragt scancktatt tlwrrnqage pvcnacglyy klhnkegiqt rnrklssksk
      601 kkkaggclgl ggvmgdmika sghldldnkp fhsgfgspms tsqhhhtmhp amqhymyhtg
      661 vgvagglhqg fappapppih phshshshsh hmtslqglql aattnamtgw rseyt
//
LOCUS       XP_034185459             569 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 33B [Osmia lignaria lignaria].
ACCESSION   XP_034185459
VERSION     XP_034185459.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329568.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185459.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..569
                     /product="vacuolar protein sorting 33B"
                     /calculated_mol_wt=63804
     Region          31..565
                     /region_name="Sec1"
                     /note="Sec1 family; pfam00995"
                     /db_xref="CDD:460020"
     CDS             1..569
                     /gene="Vps33B"
                     /coded_by="XM_034329568.2:215..1924"
                     /db_xref="GeneID:117606731"
ORIGIN      
        1 mditlddrln vlqqisqrkf veildsipgt kdlvieqrlm kildsfigvs vlkrygveki
       61 ykmeqglkls nsqhvflvss dlitckrvld qiqseislni rpclqpshhi lvapfvptvl
      121 nsiieeegls glvtlqtlcw efirldgnil slenpmftdl yyhkdttllp alahclwslq
      181 liigapkltv slgkysqqvl niiesmkqcl gssnieneig alilmdrncd latslltpvt
      241 yagllqevvd vnvgtgvlgk sqtkldpdkd qiygevrdtp csdvfpilhg kakslkseqd
      301 aiqtmklsem eryvttrlqk trdltrqlaf hisacqliad tlgsdfqtlq kiekcvleck
      361 erkecfnhie ryiddhplrt lrllcllsia sdgltqselh svqklhlhah gykhiplfyk
      421 lqsigllkcr aenilyklpn wtsewnsnaq klkllpsask qaeqkgrscp syvfnnayip
      481 liaqilnaiv nqdkepknle eianlsnsvi sgqrgpllpk mvviciiggi tyaeitacrf
      541 iekstgirlv lasdsvitgn kmlekvqei
//
LOCUS       XP_076545895             569 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 33B [Osmia lignaria lignaria].
ACCESSION   XP_076545895
VERSION     XP_076545895.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689780.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..569
                     /product="vacuolar protein sorting 33B"
                     /calculated_mol_wt=63804
     Region          31..565
                     /region_name="Sec1"
                     /note="Sec1 family; pfam00995"
                     /db_xref="CDD:460020"
     CDS             1..569
                     /gene="Vps33B"
                     /coded_by="XM_076689780.1:210..1919"
                     /db_xref="GeneID:117606731"
ORIGIN      
        1 mditlddrln vlqqisqrkf veildsipgt kdlvieqrlm kildsfigvs vlkrygveki
       61 ykmeqglkls nsqhvflvss dlitckrvld qiqseislni rpclqpshhi lvapfvptvl
      121 nsiieeegls glvtlqtlcw efirldgnil slenpmftdl yyhkdttllp alahclwslq
      181 liigapkltv slgkysqqvl niiesmkqcl gssnieneig alilmdrncd latslltpvt
      241 yagllqevvd vnvgtgvlgk sqtkldpdkd qiygevrdtp csdvfpilhg kakslkseqd
      301 aiqtmklsem eryvttrlqk trdltrqlaf hisacqliad tlgsdfqtlq kiekcvleck
      361 erkecfnhie ryiddhplrt lrllcllsia sdgltqselh svqklhlhah gykhiplfyk
      421 lqsigllkcr aenilyklpn wtsewnsnaq klkllpsask qaeqkgrscp syvfnnayip
      481 liaqilnaiv nqdkepknle eianlsnsvi sgqrgpllpk mvviciiggi tyaeitacrf
      541 iekstgirlv lasdsvitgn kmlekvqei
//
LOCUS       XP_034184870             567 aa            linear   INV 03-SEP-2025
DEFINITION  actin-related protein 8 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184870
VERSION     XP_034184870.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..567
                     /product="actin-related protein 8 isoform X1"
                     /calculated_mol_wt=63829
     Region          21..562
                     /region_name="ASKHA_NBD_Arp8-like"
                     /note="nucleotide-binding domain (NBD) of the
                     actin-related protein 8 (Arp8)-like subfamily; cd10206"
                     /db_xref="CDD:466812"
     CDS             1..567
                     /gene="Arp8"
                     /coded_by="XM_034328979.2:251..1954"
                     /db_xref="GeneID:117606482"
ORIGIN      
        1 mpvfqescae qiqaqtiiii hpgsmylrmg rasdlnpcti lnavarrrlp ggleykdsll
       61 ppavprtkel tqameesrlq vshtlqsclq sdgrrryatp pqqiaafnrr snpeisspcs
      121 vewiktdrdv vvgddilsln pddnfnihfp ykrgelnvhs gpggslrsvm adlktiweyv
      181 ltekmdiplr dlkhyravli ipdiynrqyl kelttlmlce igfgacfllq dhvaatfgag
      241 lgyacvvdvg dqktsvscve dgishrntrv rmdygggdit qtffwllqkc afpykscdps
      301 nkldalllnq lkkdfchvdl nvcgsqektf vvrkpkqqte kytlqvgdec lvaplslfqp
      361 elfkvtgthn vhiqkrstgd pedphdenyl retsrrgmke nleqtsempe eataptaage
      421 eevvvdavds apialsnrdl daprdfvvgp qqllgldhav lqsidrcpte dlkrkmyscv
      481 lvvgsgmkfq gigmwlhnri slqipymyra eqldiitqpk emdpgmtawk gaailsclep
      541 aqelwigrqe werngvrilr erapfmw
//
LOCUS       XP_034184871             494 aa            linear   INV 03-SEP-2025
DEFINITION  actin-related protein 8 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184871
VERSION     XP_034184871.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="actin-related protein 8 isoform X2"
                     /calculated_mol_wt=55770
     Region          1..489
                     /region_name="ASKHA_NBD_Arp8-like"
                     /note="nucleotide-binding domain (NBD) of the
                     actin-related protein 8 (Arp8)-like subfamily; cd10206"
                     /db_xref="CDD:466812"
     CDS             1..494
                     /gene="Arp8"
                     /coded_by="XM_034328980.2:157..1641"
                     /db_xref="GeneID:117606482"
ORIGIN      
        1 meesrlqvsh tlqsclqsdg rrryatppqq iaafnrrsnp eisspcsvew iktdrdvvvg
       61 ddilslnpdd nfnihfpykr gelnvhsgpg gslrsvmadl ktiweyvlte kmdiplrdlk
      121 hyravliipd iynrqylkel ttlmlceigf gacfllqdhv aatfgaglgy acvvdvgdqk
      181 tsvscvedgi shrntrvrmd ygggditqtf fwllqkcafp ykscdpsnkl dalllnqlkk
      241 dfchvdlnvc gsqektfvvr kpkqqtekyt lqvgdeclva plslfqpelf kvtgthnvhi
      301 qkrstgdped phdenylret srrgmkenle qtsempeeat aptaageeev vvdavdsapi
      361 alsnrdldap rdfvvgpqql lgldhavlqs idrcptedlk rkmyscvlvv gsgmkfqgig
      421 mwlhnrislq ipymyraeql diitqpkemd pgmtawkgaa ilsclepaqe lwigrqewer
      481 ngvrilrera pfmw
//
LOCUS       XP_034184872             512 aa            linear   INV 03-SEP-2025
DEFINITION  pentraxin-related protein b6 [Osmia lignaria lignaria].
ACCESSION   XP_034184872
VERSION     XP_034184872.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328981.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184872.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="pentraxin-related protein b6"
                     /calculated_mol_wt=56834
     Region          66..>190
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     CDS             1..512
                     /gene="b6"
                     /coded_by="XM_034328981.2:232..1770"
                     /db_xref="GeneID:117606483"
ORIGIN      
        1 mtaivlllcg fawaaqgist swrpsfpvsv ssvhpqtsgv fggydpeadk ctlykvsmnq
       61 elyfeyiqyk telpdmkeft lcmwsrfhnh sndhplfsya vgdqprgils wvantarssy
      121 fmmnvnghnl yrlnyplrln kwyhscqswn grtgewqiwv ndervgrgfn nrlvghvikg
      181 ggiaitgqeq rqlgggfleg egaptgsggl lgeitmvqly evaltagkah kdhkhhhahh
      241 yehetgnntp rpirppvtgp plpqnpyltg gqinhqvkln pgapvqivqg gvtlrhpali
      301 prdpppqpfp panpssapaa ftlqssqffg nlqsavspps ngiggnfpdg syhnlfkrek
      361 nsvekegeek sstkkrqied tsiehsdgkv tfvpreetdk aeelekrgse lkkrelvqlg
      421 dglivddryi sqgldndyfa gltnfglqlp kyenkddere paeaevrmim dvcdgcaeep
      481 fskalvmawr svpkklysga lhlpatplck vf
//
LOCUS       XP_034184873             511 aa            linear   INV 03-SEP-2025
DEFINITION  hydroxypyruvate reductase [Osmia lignaria lignaria].
ACCESSION   XP_034184873
VERSION     XP_034184873.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328982.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184873.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="hydroxypyruvate reductase"
                     /calculated_mol_wt=54347
     Region          8..311
                     /region_name="PGDH_4"
                     /note="Phosphoglycerate dehydrogenases, NAD-binding and
                     catalytic domains; cd12173"
                     /db_xref="CDD:240650"
     Site            order(53..54,74,76..77,101,235,285,288,294)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:240650"
     Site            order(77,101,105,151,153..155,173..176,205..207,212,
                     233..235,259..260,285,287..288)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:240650"
     Site            order(103..104,107..108,110..111,114..115,118,120..121,
                     123..124,127..128,130..132,134,137..143,145,162,165..166,
                     261,264..266,274..278,280..287,289..292,296)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:240650"
     Region          336..440
                     /region_name="PGDH_inter"
                     /note="D-3-phosphoglycerate dehydrogenase intervening
                     domain; pfam19304"
                     /db_xref="CDD:437136"
     CDS             1..511
                     /gene="LOC117606484"
                     /coded_by="XM_034328982.2:162..1697"
                     /db_xref="GeneID:117606484"
ORIGIN      
        1 mstsfgsvli sdpvhascge llarygipvt tkhklskeel ikelqnhdal ivrsetkvns
       61 evfaccpklr vvgragtgvd nidleaatrk gvvvlntpgg nsisaceltc alisalarnv
      121 aqaaqslkeg rwdrklysgf elsgktlavl gmgrigreva rrmqafgmrv lafdplltge
      181 daiqldvekl tleeiwplad yitvhtplip qtrnlinatt lakckkgvrv vnvarggivd
      241 eqalldalks ghcggaaldv fieeppknpv tlqliahpnv vatphlgast aeaqervave
      301 iaqqfialag ksteyavtgi vnapilstam tdenepwiel skklgqlaar llkgklnvtv
      361 rsqtigcgmq ekafvhtavl lgvlsgqtkn glnlinaptl aremgieveg shvsaegkav
      421 lvqveghrik gtvrdkepll lslddasfan gialgdsvcl ykgnrvqdla qivsafsaks
      481 vnvqsvnang nwiivqsdrd tsipvngies f
//
LOCUS       XP_034184874             511 aa            linear   INV 03-SEP-2025
DEFINITION  hydroxypyruvate reductase [Osmia lignaria lignaria].
ACCESSION   XP_034184874
VERSION     XP_034184874.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328983.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184874.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="hydroxypyruvate reductase"
                     /calculated_mol_wt=54347
     Region          8..311
                     /region_name="PGDH_4"
                     /note="Phosphoglycerate dehydrogenases, NAD-binding and
                     catalytic domains; cd12173"
                     /db_xref="CDD:240650"
     Site            order(53..54,74,76..77,101,235,285,288,294)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:240650"
     Site            order(77,101,105,151,153..155,173..176,205..207,212,
                     233..235,259..260,285,287..288)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:240650"
     Site            order(103..104,107..108,110..111,114..115,118,120..121,
                     123..124,127..128,130..132,134,137..143,145,162,165..166,
                     261,264..266,274..278,280..287,289..292,296)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:240650"
     Region          336..440
                     /region_name="PGDH_inter"
                     /note="D-3-phosphoglycerate dehydrogenase intervening
                     domain; pfam19304"
                     /db_xref="CDD:437136"
     CDS             1..511
                     /gene="LOC117606484"
                     /coded_by="XM_034328983.2:152..1687"
                     /db_xref="GeneID:117606484"
ORIGIN      
        1 mstsfgsvli sdpvhascge llarygipvt tkhklskeel ikelqnhdal ivrsetkvns
       61 evfaccpklr vvgragtgvd nidleaatrk gvvvlntpgg nsisaceltc alisalarnv
      121 aqaaqslkeg rwdrklysgf elsgktlavl gmgrigreva rrmqafgmrv lafdplltge
      181 daiqldvekl tleeiwplad yitvhtplip qtrnlinatt lakckkgvrv vnvarggivd
      241 eqalldalks ghcggaaldv fieeppknpv tlqliahpnv vatphlgast aeaqervave
      301 iaqqfialag ksteyavtgi vnapilstam tdenepwiel skklgqlaar llkgklnvtv
      361 rsqtigcgmq ekafvhtavl lgvlsgqtkn glnlinaptl aremgieveg shvsaegkav
      421 lvqveghrik gtvrdkepll lslddasfan gialgdsvcl ykgnrvqdla qivsafsaks
      481 vnvqsvnang nwiivqsdrd tsipvngies f
//
LOCUS       XP_034185462             476 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-30 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185462
VERSION     XP_034185462.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329571.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="sorting nexin-30 isoform X1"
                     /calculated_mol_wt=54505
     Region          82..197
                     /region_name="PX_SNX7_30_like"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexins 7 and 30; cd06860"
                     /db_xref="CDD:132770"
     Site            order(123..125,149..150,167)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132770"
     Region          221..454
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(241,244..245,249,251..252,255..256,258..259,
                     269..270,272..273,276..277,279..280,283,294,297..298,418,
                     421..422,424,429,432..433,435..436,439..440,443..444,
                     446..447,450..451,453..454)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     CDS             1..476
                     /gene="LOC117606733"
                     /coded_by="XM_034329571.2:67..1497"
                     /db_xref="GeneID:117606733"
ORIGIN      
        1 mmtssetdve klgennaedt ailevstmav ggvikqesrd tdflsncsts vegsviasps
       61 vdsfstlpeq eisdfqadsr dlqvkvdnpq khletletyi tfrittkstr pefdegeyvv
      121 rrryndfiwl rqklvdsypt hiippmpgkh tllaqldrys kefiiarmkl lhiflnrvvn
      181 hpilscdknl hifltskpae flihrknrgn vlvkmtdslq niastytmkq rhfefeqire
      241 yctalsekla tidkinhriq kdrqdyllel hqlhpiftlw atsepelapi llaiakaiec
      301 navahqklle nvpneereyi syidavkgal srrdsmqiey emtiedlakk rvekdqqlmg
      361 ivsgnlstqn wggslwkaes rdeklerlgq aiprlakqae llqdrvecan enlrsdlqrw
      421 nvekqmdlkn mlismadrqi rhyqqcmnaw eetlaglkld gtggdvnvgs sikipi
//
LOCUS       XP_034185464             476 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-30 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185464
VERSION     XP_034185464.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329573.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="sorting nexin-30 isoform X1"
                     /calculated_mol_wt=54505
     Region          82..197
                     /region_name="PX_SNX7_30_like"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexins 7 and 30; cd06860"
                     /db_xref="CDD:132770"
     Site            order(123..125,149..150,167)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132770"
     Region          221..454
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(241,244..245,249,251..252,255..256,258..259,
                     269..270,272..273,276..277,279..280,283,294,297..298,418,
                     421..422,424,429,432..433,435..436,439..440,443..444,
                     446..447,450..451,453..454)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     CDS             1..476
                     /gene="LOC117606733"
                     /coded_by="XM_034329573.2:137..1567"
                     /db_xref="GeneID:117606733"
ORIGIN      
        1 mmtssetdve klgennaedt ailevstmav ggvikqesrd tdflsncsts vegsviasps
       61 vdsfstlpeq eisdfqadsr dlqvkvdnpq khletletyi tfrittkstr pefdegeyvv
      121 rrryndfiwl rqklvdsypt hiippmpgkh tllaqldrys kefiiarmkl lhiflnrvvn
      181 hpilscdknl hifltskpae flihrknrgn vlvkmtdslq niastytmkq rhfefeqire
      241 yctalsekla tidkinhriq kdrqdyllel hqlhpiftlw atsepelapi llaiakaiec
      301 navahqklle nvpneereyi syidavkgal srrdsmqiey emtiedlakk rvekdqqlmg
      361 ivsgnlstqn wggslwkaes rdeklerlgq aiprlakqae llqdrvecan enlrsdlqrw
      421 nvekqmdlkn mlismadrqi rhyqqcmnaw eetlaglkld gtggdvnvgs sikipi
//
LOCUS       XP_034185465             475 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-30 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185465
VERSION     XP_034185465.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="sorting nexin-30 isoform X2"
                     /calculated_mol_wt=54377
     Region          82..197
                     /region_name="PX_SNX7_30_like"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexins 7 and 30; cd06860"
                     /db_xref="CDD:132770"
     Site            order(123..125,149..150,167)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132770"
     Region          221..453
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(241,244..245,249,251..252,255..256,258..259,
                     269..270,272..273,276..277,279..280,283,294,297..298,417,
                     420..421,423,428,431..432,434..435,438..439,442..443,
                     445..446,449..450,452..453)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     CDS             1..475
                     /gene="LOC117606733"
                     /coded_by="XM_034329574.2:67..1494"
                     /db_xref="GeneID:117606733"
ORIGIN      
        1 mmtssetdve klgennaedt ailevstmav ggvikqesrd tdflsncsts vegsviasps
       61 vdsfstlpeq eisdfqadsr dlqvkvdnpq khletletyi tfrittkstr pefdegeyvv
      121 rrryndfiwl rqklvdsypt hiippmpgkh tllaqldrys kefiiarmkl lhiflnrvvn
      181 hpilscdknl hifltskpae flihrknrgn vlvkmtdslq niastytmkq rhfefeqire
      241 yctalsekla tidkinhriq kdrqdyllel hqlhpiftlw atsepelapi llaiakaiec
      301 navahqklle nvpneereyi syidavkgal srrdsmqiey emtiedlakk rvekdqlmgi
      361 vsgnlstqnw ggslwkaesr deklerlgqa iprlakqael lqdrvecane nlrsdlqrwn
      421 vekqmdlknm lismadrqir hyqqcmnawe etlaglkldg tggdvnvgss ikipi
//
LOCUS       XP_034185466             445 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin-30 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185466
VERSION     XP_034185466.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329575.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="sorting nexin-30 isoform X3"
                     /calculated_mol_wt=51319
     Region          51..166
                     /region_name="PX_SNX7_30_like"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexins 7 and 30; cd06860"
                     /db_xref="CDD:132770"
     Site            order(92..94,118..119,136)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132770"
     Region          190..423
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(210,213..214,218,220..221,224..225,227..228,
                     238..239,241..242,245..246,248..249,252,263,266..267,387,
                     390..391,393,398,401..402,404..405,408..409,412..413,
                     415..416,419..420,422..423)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     CDS             1..445
                     /gene="LOC117606733"
                     /coded_by="XM_034329575.2:61..1398"
                     /db_xref="GeneID:117606733"
ORIGIN      
        1 mmtssetdve klgennaedt aileiaspsv dsfstlpeqe isdfqadsrd lqvkvdnpqk
       61 hletletyit frittkstrp efdegeyvvr rryndfiwlr qklvdsypth iippmpgkht
      121 llaqldrysk efiiarmkll hiflnrvvnh pilscdknlh ifltskpaef lihrknrgnv
      181 lvkmtdslqn iastytmkqr hfefeqirey ctalseklat idkinhriqk drqdyllelh
      241 qlhpiftlwa tsepelapil laiakaiecn avahqkllen vpneereyis yidavkgals
      301 rrdsmqieye mtiedlakkr vekdqqlmgi vsgnlstqnw ggslwkaesr deklerlgqa
      361 iprlakqael lqdrvecane nlrsdlqrwn vekqmdlknm lismadrqir hyqqcmnawe
      421 etlaglkldg tggdvnvgss ikipi
//
LOCUS       XP_034184875             456 aa            linear   INV 03-SEP-2025
DEFINITION  ruvB-like helicase pontin [Osmia lignaria lignaria].
ACCESSION   XP_034184875
VERSION     XP_034184875.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328984.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..456
                     /product="ruvB-like helicase pontin"
                     /calculated_mol_wt=49926
     Region          14..368
                     /region_name="TIP49"
                     /note="TIP49 P-loop domain; pfam06068"
                     /db_xref="CDD:399217"
     Region          374..439
                     /region_name="TIP49_C"
                     /note="TIP49 AAA-lid domain; pfam17856"
                     /db_xref="CDD:436097"
     CDS             1..456
                     /gene="pont"
                     /coded_by="XM_034328984.2:117..1487"
                     /db_xref="GeneID:117606485"
ORIGIN      
        1 mkieevksta ktqrisahth ikglgldeng iaiqsaaglv gqemareaag vvvdmikskk
       61 magravllag ppgtgktaia laiaqelgnk vpfcpmvgse vysseikkte vlmenfrrai
      121 glriketkev yegevteltp vetenpmggy gktvshvvig lktakgtkql kldpsiyesl
      181 qkekvetgdv iyveansgav krqgrsdnfa tefdleaeey vplpkgdvhk kkeviqdvtl
      241 hdldvanakp qggqdvmsmm gqlmkpkkte itdklrkein kvvnkyidqg vaelvpgvlf
      301 idevhmldie tftylhrale saiapivifa tnrgrcvirg tedivsphgi pldlldrlli
      361 irtlpysrse ieqivklrat teglqiedea lsnlgelgtk ttlryvvqll tpaaltakvn
      421 ertvikkedv eevaslflda kssakiltqn kdkfmk
//
LOCUS       XP_034184876             445 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606486 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184876
VERSION     XP_034184876.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328985.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="uncharacterized protein LOC117606486 isoform X1"
                     /calculated_mol_wt=49836
     Region          226..280
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(226..229,232..233,240,252..253,256,259..260,267,271,
                     274,278)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..445
                     /gene="LOC117606486"
                     /coded_by="XM_034328985.2:157..1494"
                     /db_xref="GeneID:117606486"
ORIGIN      
        1 mmhtnirkpa ksektprrkk lteqqsdamd mfvtpkrqkn ddlevtgisr sgrvrkkssk
       61 lvdfespddf adnkykrqka qqlqsqqfld rydsqhispn qsiqhgsggr qrrnsnsstg
      121 qerikteils dneeqssese sddpsglned erysidsgsd ddvdslmidd reagfrklep
      181 pgqetpsqan slymlekckk kliikdgkii grmkaqrkdk gktrftayml wakeirqell
      241 eqcpymdfaa iskrlgelwa tvpnlekynw rrrakrlaak phslpaskde pvwkmpppas
      301 rkkfinkign gkeqkpatsk rtiqlglpsv vgnvpvspps nrtgkdlvne piigtgmykv
      361 vgtqpidvaa hlkllgeslt iigerlkehd gqiavsgsls vlldsllcal gplicltqqi
      421 petngakhet lsqmldniay lmpgl
//
LOCUS       XP_034184877             443 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606486 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184877
VERSION     XP_034184877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328986.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..443
                     /product="uncharacterized protein LOC117606486 isoform X2"
                     /calculated_mol_wt=49608
     Region          224..278
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(224..227,230..231,238,250..251,254,257..258,265,269,
                     272,276)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..443
                     /gene="LOC117606486"
                     /coded_by="XM_034328986.1:157..1488"
                     /db_xref="GeneID:117606486"
ORIGIN      
        1 mmhtnirkpa ksektprrkk lteqqsdamd mfvtpkrqkn devtgisrsg rvrkkssklv
       61 dfespddfad nkykrqkaqq lqsqqfldry dsqhispnqs iqhgsggrqr rnsnsstgqe
      121 rikteilsdn eeqssesesd dpsglneder ysidsgsddd vdslmiddre agfrkleppg
      181 qetpsqansl ymlekckkkl iikdgkiigr mkaqrkdkgk trftaymlwa keirqelleq
      241 cpymdfaais krlgelwatv pnlekynwrr rakrlaakph slpaskdepv wkmpppasrk
      301 kfinkigngk eqkpatskrt iqlglpsvvg nvpvsppsnr tgkdlvnepi igtgmykvvg
      361 tqpidvaahl kllgesltii gerlkehdgq iavsgslsvl ldsllcalgp licltqqipe
      421 tngakhetls qmldniaylm pgl
//
LOCUS       XP_034184878             422 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606486 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184878
VERSION     XP_034184878.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328987.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="uncharacterized protein LOC117606486 isoform X3"
                     /calculated_mol_wt=47445
     Region          <200..>315
                     /region_name="NHP6B"
                     /note="Chromatin-associated proteins containing the HMG
                     domain [Chromatin structure and dynamics]; COG5648"
                     /db_xref="CDD:227935"
     Region          226..280
                     /region_name="HMG-box_SF"
                     /note="high mobility group (HMG)-box domain superfamily;
                     cd00084"
                     /db_xref="CDD:438789"
     Site            order(226..229,232..233,240,252..253,256,259..260,267,271,
                     274,278)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438789"
     CDS             1..422
                     /gene="LOC117606486"
                     /coded_by="XM_034328987.2:157..1425"
                     /db_xref="GeneID:117606486"
ORIGIN      
        1 mmhtnirkpa ksektprrkk lteqqsdamd mfvtpkrqkn ddlevtgisr sgrvrkkssk
       61 lvdfespddf adnkykrqka qqlqsqqfld rydsqhispn qsiqhgsggr qrrnsnsstg
      121 qerikteils dneeqssese sddpsglned erysidsgsd ddvdslmidd reagfrklep
      181 pgqetpsqan slymlekckk kliikdgkii grmkaqrkdk gktrftayml wakeirqell
      241 eqcpymdfaa iskrlgelwa tvpnlekynw rrrakrlaak phslpaskde pvwkmpppas
      301 rkkfinkign vpvsppsnrt gkdlvnepii gtgmykvvgt qpidvaahlk llgesltiig
      361 erlkehdgqi avsgslsvll dsllcalgpl icltqqipet ngakhetlsq mldniaylmp
      421 gl
//
LOCUS       XP_034184869             569 aa            linear   INV 03-SEP-2025
DEFINITION  sodium-independent sulfate anion transporter [Osmia lignaria
            lignaria].
ACCESSION   XP_034184869
VERSION     XP_034184869.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328978.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184869.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..569
                     /product="sodium-independent sulfate anion transporter"
                     /calculated_mol_wt=62076
     Region          38..551
                     /region_name="SUL1"
                     /note="Sulfate permease or related transporter, MFS
                     superfamily [Inorganic ion transport and metabolism];
                     COG0659"
                     /db_xref="CDD:440424"
     CDS             1..569
                     /gene="LOC117606481"
                     /coded_by="XM_034328978.2:175..1884"
                     /db_xref="GeneID:117606481"
ORIGIN      
        1 mlledtrrtf gakmarcgkt dcneytkrrl pilswltsyk lawlpqdala gftvgltaip
       61 qgiaygvvag lspeyglyss fmasfvyivf gsctsitigp taimatmtqs lvlkygsdia
      121 vlltflkgci itslgllhlg fllnfislpv itgftvaasi niaasqfkal lgipgrsddf
      181 mdslisvfsk ldeiryqdtl lgiatiamlv lfknlpgrrt gnwmqkaawt vtlarnalvv
      241 iagtvmayvf finnqtpfvl tgtmgeglpp fgpppfiiaa nnrtydllet vvamgptlaf
      301 vpivsaiehm aiakafakgk sldatqemfa lglcnmfgsf vrsmpitgsf trtavnyssg
      361 vkttfgglft gclvllaagl ltstfrfipk atlagvvica myymldfkay relfnarkld
      421 falmmvtllf svflklewgi iigiilnlii llyfstrstv qtetqqlgdk tviriipeet
      481 vtfpaaenfr ahimelsedk lcsdvvldcq nlkridvtva nnlkllandl rlrgqtircv
      541 ncrdnvqavl kaiapdlqcg ssndenlyq
//
LOCUS       XP_034184880             351 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein S22 [Osmia lignaria lignaria].
ACCESSION   XP_034184880
VERSION     XP_034184880.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328989.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184880.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..351
                     /product="mitochondrial ribosomal protein S22"
                     /calculated_mol_wt=41044
     Region          41..282
                     /region_name="MRP-S22"
                     /note="Mitochondrial 28S ribosomal protein S22; pfam10245"
                     /db_xref="CDD:431167"
     CDS             1..351
                     /gene="mRpS22"
                     /coded_by="XM_034328989.2:45..1100"
                     /db_xref="GeneID:117606488"
ORIGIN      
        1 mmsnklnfll rqfyrrpawn crfcstippt agnekdlapm ffdervqqll vtltrinyer
       61 ifaprrdgtp ltsptykfmt deelkeakle vekkargriq mppvvnprdd tdlvlskdya
      121 iqgfdntkyv ftdisfggtn rhrvivvrep sgilrrantd erhrmnqvyf pikdrelhtp
      181 kmftnphfse llekeefefi ldraclqydp dhseyhritk avysrvqewk kfdalrstrh
      241 fgplvfhlas ekniealled vikngkieeg aalvelyhcl hpeaqsagkp tqndielirl
      301 yaeldssdry gieraletye hiqaekkqlq erieqghhva eetdqsdqpk t
//
LOCUS       XP_034184881             326 aa            linear   INV 03-SEP-2025
DEFINITION  adhesion G protein-coupled receptor D2 [Osmia lignaria lignaria].
ACCESSION   XP_034184881
VERSION     XP_034184881.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328990.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184881.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="adhesion G protein-coupled receptor D2"
                     /calculated_mol_wt=36468
     Region          44..>186
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     Site            order(82,165,167,170,175)
                     /site_type="active"
                     /note="calcium mediated ligand binding site [active]"
                     /db_xref="CDD:238086"
     CDS             1..326
                     /gene="LOC117606489"
                     /coded_by="XM_034328990.2:160..1140"
                     /db_xref="GeneID:117606489"
ORIGIN      
        1 mkmsrnnfll lllglaicpc sggqkqretp mhkaqltqkg yiqflrwqlp vpriteftfc
       61 lwiqsddlth phsifsyskn ererlvrawi sprgrsvhle iggkqviast teidenrwyh
      121 lclswenqvg lyglwingql wargqseeta ghaipsggdi vlgqeytdfd kgleegvegs
      181 vlgfnlllas afdplardyq hreasssssy anaplfarip vklpqpgfdy aartlpvsnq
      241 prarryadsf qlpltnrtag ykdkvigsnk eplglqlvkl syvrceigrg spfigghlml
      301 iswsrtpvrv fggailknvk secgnf
//
LOCUS       XP_034184882             311 aa            linear   INV 03-SEP-2025
DEFINITION  U3 small nucleolar ribonucleoprotein IMP4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184882
VERSION     XP_034184882.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328991.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="U3 small nucleolar ribonucleoprotein IMP4"
                     /calculated_mol_wt=36401
     Region          97..264
                     /region_name="Brix"
                     /note="The Brix domain is found in a number of eukaryotic
                     proteins; smart00879"
                     /db_xref="CDD:214879"
     CDS             1..311
                     /gene="LOC117606490"
                     /coded_by="XM_034328991.2:195..1130"
                     /db_xref="GeneID:117606490"
ORIGIN      
        1 mlrrqarlrr eylyrksvqd klkniqekke klkrsleenv pihpdlrkda lniqqkldwe
       61 dagpemamam gtemggtlgs heddeyrwag vedpkivitt srdpssrlkm fakelrlifp
      121 nsqrmnrgny emkqlvhacr andvtdfiiv hehrgvpdsl iichlpygpt ayftmsdvvm
      181 rhdipnigtm seqyphlifh nfktklgtrv msilkylfpv pkedskriit fanhddyisf
      241 rhhtykkihg kdiemtevgp rfqlklyeik lgtldaesaa dvewalrpym ntthkrrfls
      301 dedgwqqedn i
//
LOCUS       XP_034184883             241 aa            linear   INV 03-SEP-2025
DEFINITION  cation-dependent mannose-6-phosphate receptor [Osmia lignaria
            lignaria].
ACCESSION   XP_034184883
VERSION     XP_034184883.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328992.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="cation-dependent mannose-6-phosphate receptor"
                     /calculated_mol_wt=26551
     Region          47..234
                     /region_name="ATG27"
                     /note="Autophagy-related protein 27; cl39051"
                     /db_xref="CDD:476860"
     CDS             1..241
                     /gene="LOC117606492"
                     /coded_by="XM_034328992.2:76..801"
                     /db_xref="GeneID:117606492"
ORIGIN      
        1 mfgpawnliv cvcfftlfce qtssectqlt pcscmfpdgh gydlsplrkl splqtindsy
       61 tfyfhpctnk plsvnvtsec fkgdgvslca qhgnttinlg rvedthieaa sdqvkpslii
      121 hhenytssvt lfccdsctss hiimetkign sefrlllvsp yackvsirpk glsigsilvl
      181 nffiitgvyf iggamalkfl rgatgwemvp nhtfwgelss lvkdgiaftf sccridsydr
      241 i
//
LOCUS       XP_034184884             227 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606493 [Osmia lignaria lignaria].
ACCESSION   XP_034184884
VERSION     XP_034184884.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184884.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..227
                     /product="uncharacterized protein LOC117606493"
                     /calculated_mol_wt=26085
     CDS             1..227
                     /gene="LOC117606493"
                     /coded_by="XM_034328993.2:256..939"
                     /db_xref="GeneID:117606493"
ORIGIN      
        1 meftledtle vlkdtqaerq reiddlmnii lnnnpvalnn asnpedvrkk qqnfyrslly
       61 hvsreedndl pivrtpdmhi qvlseleeev easqklldym kaelseined iscleskqqg
      121 lekmkegyav taetkagtty mkeqvithrm fkevksdlsa vvdvifpknp efkyfladlv
      181 lsyskggnel yvdvtpdvly fvnfligani avyhrndknk vrltepl
//
LOCUS       XP_034184885             215 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606494 [Osmia lignaria lignaria].
ACCESSION   XP_034184885
VERSION     XP_034184885.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="uncharacterized protein LOC117606494"
                     /calculated_mol_wt=23107
     CDS             1..215
                     /gene="LOC117606494"
                     /coded_by="XM_034328994.2:453..1100"
                     /db_xref="GeneID:117606494"
ORIGIN      
        1 mklalfalva igylvavvrs veeeydyeee paapvtppps rsnsprlggl lssrgrvnvp
       61 rktsaaaatq sttakavepa veaeeeadee yeenqeqeeq tpttttessk kvrggvrpfr
      121 snqdllaalk rrraqvgpss shrdssatqs atesttaksk gattsrnksn ngaseskssg
      181 rgrfgatrgs kpvqeeveet qreevqvkpk pyrrg
//
LOCUS       XP_034184887             170 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606495 [Osmia lignaria lignaria].
ACCESSION   XP_034184887
VERSION     XP_034184887.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328996.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..170
                     /product="uncharacterized protein LOC117606495"
                     /calculated_mol_wt=19780
     CDS             1..170
                     /gene="LOC117606495"
                     /coded_by="XM_034328996.2:123..635"
                     /db_xref="GeneID:117606495"
ORIGIN      
        1 mgaygknmnt hqdwrnsltd sssgdavkyn lrcnrnisek mtkkdfvydi rppiqstrkl
       61 eeikqpivls lqlrkkensd skvpqyavis erdkhagsgd afnlseqrsl rfecnfcheq
      121 iatllslsth vkfhrrkyck ycywipmdne tmeehfesih ridsivttsv
//
LOCUS       XP_034184879             418 aa            linear   INV 03-SEP-2025
DEFINITION  trissin receptor-like [Osmia lignaria lignaria].
ACCESSION   XP_034184879
VERSION     XP_034184879.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..418
                     /product="trissin receptor-like"
                     /calculated_mol_wt=47204
     Region          46..344
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          47..71
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320140"
     Region          80..102
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320140"
     Region          118..140
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320140"
     Region          163..179
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320140"
     Region          209..232
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320140"
     Region          275..297
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320140"
     Region          313..338
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320140"
     CDS             1..418
                     /gene="LOC117606487"
                     /coded_by="XM_034328988.2:858..2114"
                     /db_xref="GeneID:117606487"
ORIGIN      
        1 mnyqstrhtv myeipgnetv egnetllsnv slneeyifdr tdvkvifitl ysivfvccff
       61 gnllvilvvt fsrrlrsitn fflanlavad fcvgifcvyq tltnylmnsw llgdflckvy
      121 mfvhalsyta sililvvvcv erylaivhpi kcrsvltrrr lqlvigvvwm iaaiyasprf
      181 fyvetmrnrl itgdvdiicl anikkhnkav ldavnlvfly lvplalmchl ysrlavglwk
      241 ssatlsgaas narngkshri qtssrnvlra rrgvirmlia vvmmfavcnl pqqariawgy
      301 wdsnydrtsd fstlltlstf lisyvnscln pllyaflsrn fqkgtrelfn cygkrrkrsg
      361 agssalamgc trgdtstrqe nsmnaniaqa slirtssgqd asrtsrtffr qttceksm
//
LOCUS       XP_034192452             652 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609839 [Osmia lignaria lignaria].
ACCESSION   XP_034192452
VERSION     XP_034192452.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336561.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192452.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..652
                     /product="uncharacterized protein LOC117609839"
                     /calculated_mol_wt=74819
     Region          <228..619
                     /region_name="RAG1"
                     /note="Recombination-activation protein 1 (RAG1),
                     recombinase; pfam12940"
                     /db_xref="CDD:315595"
     CDS             1..652
                     /gene="LOC117609839"
                     /coded_by="XM_034336561.2:440..2398"
                     /db_xref="GeneID:117609839"
ORIGIN      
        1 mkqdirkllh dlkvkwqqvh rmeerffnkn rewldvfvvf tkeeeiaets tkkdsgrpss
       61 dfstssertk rrrtegirsk tnveelcyat qmnlraagql daakvlqdmt vspskaqkyr
      121 kcvqsisent ftgdsalsll ielkfsrsqy qlqrsaclqn nckvfpsykk vqeakhncyp
      181 pksemtiten cgeiklqall dhtaerillt qidvikslsp eqvqnmilic kwgcdgssgq
      241 stykrkfsdd sgsqsdanvf ftslvplqll stneennkiv iwknprpssp rfcrpikikf
      301 lhettegtvk etddikrqes elttfktifd gkcisvvyql altmidgkvc naitgtlstq
      361 rcylcgatsk dfndidavlq ktvnvtnlqf glstlhawir ffecclhlsy kldlqkwqsr
      421 needklqver rkrniqegfr vklglivdkp kpgygstndg ntarrffens svsapitgvd
      481 edlikrfhti lqtiscgyei nlpkfqqytl ntarkfvhly pwyymptsvh kllmhgteii
      541 dssilpigqm sedaqeschk yfkrfredfs rkcsrtknme dvllrmlvss dpfvssqrql
      601 pkkklrslss eaiellippk vrnvtsdtie ninipqsstd ensdnstddd ey
//
LOCUS       XP_034192335             105 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609777 [Osmia lignaria lignaria].
ACCESSION   XP_034192335
VERSION     XP_034192335.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336444.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192335.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="uncharacterized protein LOC117609777"
                     /calculated_mol_wt=11508
     CDS             1..105
                     /gene="LOC117609777"
                     /coded_by="XM_034336444.2:1..318"
                     /db_xref="GeneID:117609777"
ORIGIN      
        1 mycftrdfsy edtanevydv gkdedilstn klllfaddsg ssrlqnaggv neagsaivvq
       61 idvakfgkrk ynrgrivdgh wvlgmvdtst ndcgmvvvqd grrqh
//
LOCUS       XP_076545896             240 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305596 [Osmia lignaria lignaria].
ACCESSION   XP_076545896
VERSION     XP_076545896.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689781.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 11% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="uncharacterized protein LOC143305596"
                     /calculated_mol_wt=27828
     Region          <65..240
                     /region_name="COG3676"
                     /note="Transposase and inactivated derivatives [Mobilome:
                     prophages, transposons]"
                     /db_xref="CDD:442892"
     CDS             1..240
                     /gene="LOC143305596"
                     /coded_by="XM_076689781.1:582..1304"
                     /db_xref="GeneID:143305596"
ORIGIN      
        1 miityafawk fsfgqtqhec siidgqtvss etiidrfsfc revctialdk kyetegllgg
       61 pgiiveidec kigrrkfekg rfreglwilg tldiylkifr iqvrlgrmie rggnkyrlei
      121 cpnnkrdett llaliqkhia igseihtdcw kgyqnlnrhg yihktvnhse nfvdpeigay
      181 tqniesswrs lrarlsrggi hqddlidhlc eylwryevkk nggdpfhhll edikyvyngn
//
LOCUS       XP_076545897             333 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305597 [Osmia lignaria lignaria].
ACCESSION   XP_076545897
VERSION     XP_076545897.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689782.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="uncharacterized protein LOC143305597"
                     /calculated_mol_wt=37138
     CDS             1..333
                     /gene="LOC143305597"
                     /coded_by="XM_076689782.1:1..1002"
                     /db_xref="GeneID:143305597"
ORIGIN      
        1 mplarsptrv sdrtasgsdl pvadpsrmek sestsaaage vnavarvklp pfwkensllw
       61 fsqveiafal srivsddtkf rnvvlvldas vlrvvadvil sppetgkyds lrrrilesfd
      121 essesklrrl lrghsmgdek pssylqrlrn laggqcsesi lktlfleqmp enfrvilais
      181 egmdlgklvl qadklfeivs pgvaavadah psnvdivaat sktstdlseq lseilsrlrr
      241 leardagrdd rfrrrdrksn rpeerrarsk shdglcrlha kygadarhcf ppckwtnqgr
      301 renyspqclr rqtfqglssv asssetrhqa sif
//
LOCUS       XP_076545898             196 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305599 [Osmia lignaria lignaria].
ACCESSION   XP_076545898
VERSION     XP_076545898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689783.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="uncharacterized protein LOC143305599"
                     /calculated_mol_wt=22761
     Region          3..155
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     CDS             1..196
                     /gene="LOC143305599"
                     /coded_by="XM_076689783.1:1..591"
                     /db_xref="GeneID:143305599"
ORIGIN      
        1 mdtlffsdea wfhlngyins qnsrtwsttn pheffeqplh sskvgvwcai srkrivgpff
       61 fertvtsevy eqiiiqfiss lepnerhcwf qqdgapahta astmqllhef fgdqiisrgl
      121 wpprspdltp pdfflwgtlk cqvyannprt leelkeniqk sileitedtl kkvfenmrrr
      181 isiclaqngg hfqhll
//
LOCUS       XP_076545899             176 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611087 [Osmia lignaria lignaria].
ACCESSION   XP_076545899
VERSION     XP_076545899.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689784.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..176
                     /product="uncharacterized protein LOC117611087"
                     /calculated_mol_wt=19877
     CDS             1..176
                     /gene="LOC117611087"
                     /coded_by="XM_076689784.1:1..531"
                     /db_xref="GeneID:117611087"
ORIGIN      
        1 maskmektdn vedkkaedae pllqlqqllq qqslqmqqnq telatlrqqv qelgerqhag
       61 lipqpqhqpk hqpphqsphq pphqppqpqh qpthptyhsp laglygpeqp tayrseqrlf
      121 rqrrrearrl wrqstcrdgc grgrgrsycp csgpcgcthn cgagrsscsc ccgrht
//
LOCUS       XP_076545900             515 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305600 [Osmia lignaria lignaria].
ACCESSION   XP_076545900
VERSION     XP_076545900.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689785.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="uncharacterized protein LOC143305600"
                     /calculated_mol_wt=60693
     CDS             1..515
                     /gene="LOC143305600"
                     /coded_by="XM_076689785.1:1..1548"
                     /db_xref="GeneID:143305600"
ORIGIN      
        1 mredevvrvi ryveliilya nkqcqkygth qhhyqmirtr lrllgrflns ikeidsrien
       61 fadvfqpryy datidtllkr vkdflkllse nygisinrai aeiqtqnnrr kkvifpttdd
      121 ikkldsflks krsqyyqklr ekfsyeiwkk laettllslq lfnrrrpgei erlyledfrs
      181 yrsidqmadk etyntlsqeg kalarryvrf eiggklgrav pvlihvdlld clnliiqhrs
      241 sagvpsqney lfglpssdkd knrylracdl mrkysiqcga eitsslrgtq lrkhiatkci
      301 nlnltdgqvt dlanfmghhk kihkdiyrqp vletdivkis kilqtaqgln eseeesdsne
      361 dfsiqstsqd rsnasnrlhk ksvmthstpr rwsnmertvt lsafpeflsn gkipsgkelq
      421 kliddhkqtm mnmmlqlrqq lteqqqqhqr qqellhqqvh alqqqlqqlv qqqqqqrpqq
      481 qrqpqqqqhe tnkedakplr rkyhrshqtn ntveq
//
LOCUS       XP_076545901             138 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305601 [Osmia lignaria lignaria].
ACCESSION   XP_076545901
VERSION     XP_076545901.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689786.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 32% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..138
                     /product="uncharacterized protein LOC143305601"
                     /calculated_mol_wt=15808
     Region          66..138
                     /region_name="Dimer_Tnp_hAT"
                     /note="hAT family C-terminal dimerization region;
                     pfam05699"
                     /db_xref="CDD:399013"
     CDS             1..138
                     /gene="LOC143305601"
                     /coded_by="XM_076689786.1:1..417"
                     /db_xref="GeneID:143305601"
ORIGIN      
        1 mqfknhllal teqkfkdlef npvfaistml dlrfkklhfq salaassait kintlirnde
       61 ealnlelrqy inlpvisrye nslkhweivk saypslckla mkylsvvats vpserlfsra
      121 rtikaenrsr lsgehlnq
//
LOCUS       XP_034192177             163 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609673 [Osmia lignaria lignaria].
ACCESSION   XP_034192177
VERSION     XP_034192177.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336286.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192177.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..163
                     /product="uncharacterized protein LOC117609673"
                     /calculated_mol_wt=18828
     Region          <5..133
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..163
                     /gene="LOC117609673"
                     /coded_by="XM_034336286.2:1..492"
                     /db_xref="GeneID:117609673"
ORIGIN      
        1 mtgdyhgqmn alnfekwine kllpnipshs iivmdnapyh tvqinkppta ygkksdmikw
       61 leenniqhaa tmkkyqlvel iqrnksldkt ykvdelfkrh ghnvlrlppy mcelnpiela
      121 wakveriire nntstltaie ltktekainv vstedwvgfc khv
//
LOCUS       XP_076545902             559 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305602 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545902
VERSION     XP_076545902.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689787.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 50% of CDS bases
            frameshifts :: corrected 3 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..559
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305602"
                     /calculated_mol_wt=61532
     Region          235..384
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..559
                     /gene="LOC143305602"
                     /coded_by="XM_076689787.1:1..1680"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     deleted 3 bases in 2 codons"
                     /db_xref="GeneID:143305602"
ORIGIN      
        1 meatertqtr ttpegasrpa tdpqplprkg atldfkttsp sspddataek sppgaicrra
       61 rrlqptpvtt edpsapstpi tveeeatner vwvrpwinrr nslgmssrsl lkelavedka
      121 eyfntlrmte esfnillekv tekxqrkdth irsaipakik lqtilyflat gcslrtlqhl
      181 frlgkstise fipevceavs nslkdfikvp denewkcien gfrkewnfpg ccgaidgkhv
      241 aikapadcgs lyykqsnsiv lmavvdhnyc fkyidvgcng risdggvfrh cnlyqalesg
      301 mppsnhflvg dnafpmkeyl lkpfpdcrlt vkqrvfnyrl srarrvvena fgilasrfri
      361 fekpmpfcpd kvsimvktcy tlhnwlrvsa pqhvhsvdae nfesggliqg swrssqpplg
      421 llpldgedkk peeaepllqp qqllqqqslq mqqnktelat lrqqiqelkg iqavipatpq
      481 rgknsgcsgn aaerqggcgg kqcavtvaaa aavtapaaar adarrtaara avavlaaadg
      541 tlevsttiis vyvimtkii
//
LOCUS       XP_076545904             171 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305603 [Osmia lignaria lignaria].
ACCESSION   XP_076545904
VERSION     XP_076545904.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689789.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 26% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="uncharacterized protein LOC143305603"
                     /calculated_mol_wt=18908
     Region          <68..170
                     /region_name="transpos_IS1595"
                     /note="IS1595 family transposase; NF033547"
                     /db_xref="CDD:468078"
     CDS             1..171
                     /gene="LOC143305603"
                     /coded_by="XM_076689789.1:3..518"
                     /db_xref="GeneID:143305603"
ORIGIN      
        1 mycftsdfsy evtatevsdv gedeevlttd tiaawfrycr emivdhvckm qagkpklgai
       61 deggsaivvq ideakfgkrk ynrgriidgh wvlgmvdtst ndcrmvvvqd reaatlipli
      121 kenvaagsei htdslrsymg laaerfihkm vnlsvefvap dgthtqqiea n
//
LOCUS       XP_076545905             131 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305604 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545905
VERSION     XP_076545905.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689790.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 20% of CDS bases
            frameshifts :: corrected 2 indels
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..131
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305604"
                     /calculated_mol_wt=14495
     CDS             1..131
                     /gene="LOC143305604"
                     /coded_by="XM_076689790.1:1..396"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     deleted 1 base in 1 codon"
                     /db_xref="GeneID:143305604"
ORIGIN      
        1 mvaefvkndd ryniaemrlv kaslssresy gdsaigyvcl rrelsxlhke gkicpehrvk
       61 skryavtlvv neadetiqev ncedcavsqg gckpalaflm wlhcknscpq dksgdlqpqp
      121 kpqtepllql q
//
LOCUS       XP_076545906             315 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305605 [Osmia lignaria lignaria].
ACCESSION   XP_076545906
VERSION     XP_076545906.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689791.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 21% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="uncharacterized protein LOC143305605"
                     /calculated_mol_wt=35220
     Region          204..>268
                     /region_name="YqaJ"
                     /note="YqaJ-like viral recombinase domain; cl09232"
                     /db_xref="CDD:447697"
     Region          249..>304
                     /region_name="PDDEXK_nuclease-like"
                     /note="PDDEXK family nucleases; cl40440"
                     /db_xref="CDD:477358"
     CDS             1..315
                     /gene="LOC143305605"
                     /coded_by="XM_076689791.1:1..948"
                     /db_xref="GeneID:143305605"
ORIGIN      
        1 mvepgfvkad stnlpnidvf mvaefvkndd rynaaeirlv kaslssresy gdiaigyicl
       61 rressictik gkicpehrvk skryavtlvv neadesiqev ncedcaasqg gckhalaflm
      121 wlhrkseqrs ptevecywnk pmlgtvgttk kfveatelck eeipstvlpd nstflenilk
      181 eaeekqidsq lsrhnfqlle recyslslhq lviaflkngg sgandftqfa tskmtaqlis
      241 kveietrqqs esslwyelry gritasvmhe aahckttdgs ftnkilgaak kfnsaltrrg
      301 knlensiqnl kqagk
//
LOCUS       XP_076545907             271 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305606 [Osmia lignaria lignaria].
ACCESSION   XP_076545907
VERSION     XP_076545907.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689792.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..271
                     /product="uncharacterized protein LOC143305606"
                     /calculated_mol_wt=29640
     CDS             1..271
                     /gene="LOC143305606"
                     /coded_by="XM_076689792.1:1..816"
                     /db_xref="GeneID:143305606"
ORIGIN      
        1 mtdkekmeag taaevnasdf rsvklptfwk ddpklwfaml ekeflaygvr sdvvkcasvl
       61 rhldgniirv vadiisapec edsyrrikea lierlassee tqlrqlltgi elngrkptel
      121 lremqllaga kvsasvlqtl wlqrlptrvq ellavvddaa ldklaaladk mierssfpev
      181 aaitesepnt hrgtapqdpe aaaeadslrd vappaeeali vtaklrdqii vfiigslesd
      241 hlnalspaag qnrtrvrsrk tkqvvasgdg c
//
LOCUS       XP_034194886             236 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117611098 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194886
VERSION     XP_034194886.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338995.2
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194886.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 1% of CDS bases
            frameshifts          :: corrected 3 indels
            internal stop codons :: corrected 2 genomic stop codons
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117611098"
                     /calculated_mol_wt=26946
     Region          5..98
                     /region_name="THAP"
                     /note="THAP domain; pfam05485"
                     /db_xref="CDD:461662"
     CDS             1..236
                     /gene="LOC117611098"
                     /coded_by="XM_034338995.2:1..711"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 2 codons;
                     deleted 1 base in 1 codon; substituted 2 bases at 2
                     genomic stop codons"
                     /db_xref="GeneID:117611098"
ORIGIN      
        1 mprrcwfpnc kgnydstlkd nnytsifllp kdeelrkkwt aaiprknxtp tkysavcslh
       61 fsetdixryq iilsptgvle nvllrcprli anavlsifpn spsyltkhvl pqrkdpaerq
      121 erviynndnl vqqfiesdli ssftdlvnty kekliiancw nikixkfnfy mlvsnndsdf
      181 ltvttqivia ddmtlqicee gnklsfndlk wvlpidkkls rwsxlqnilt rykvkv
//
LOCUS       XP_034194533             375 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610812 [Osmia lignaria lignaria].
ACCESSION   XP_034194533
VERSION     XP_034194533.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338642.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..375
                     /product="uncharacterized protein LOC117610812"
                     /calculated_mol_wt=41973
     Region          3..199
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(11,83,116,118,166,191..192)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197311"
     Site            order(11,191)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197311"
     Site            order(83,118,192)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..375
                     /gene="LOC117610812"
                     /coded_by="XM_034338642.1:1..1128"
                     /db_xref="GeneID:117610812"
ORIGIN      
        1 mgqtditliq kpwihkervg glntgcdsly ydatstrpra cifvkkkiaa lklnefctad
       61 lsavkvklnl sgntselvvc saylpydsed ppptrelral idhcavnglh lvigcdansh
      121 htvwgstntn grgtallayf attsleilni gssptfvtsc rqevidltlg sakvtqvikh
      181 wqvrdentls nhrlitfelt gdrdggtgea yrnpkatnwa lykedlkgrl krncqrprti
      241 geieqaveyl ssaitqayea acpariryss krvpcwnkkl dqarkdtrkl lnkamkante
      301 pawdkykksq qaykklirts ertawktfck etealpvvar lrkvftigps prldrlrlpn
      361 ggltnnhkes lehli
//
LOCUS       XP_034195203             244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611378 [Osmia lignaria lignaria].
ACCESSION   XP_034195203
VERSION     XP_034195203.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339312.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..244
                     /product="uncharacterized protein LOC117611378"
                     /calculated_mol_wt=27508
     Region          164..>244
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..244
                     /gene="LOC117611378"
                     /coded_by="XM_034339312.1:1..735"
                     /db_xref="GeneID:117611378"
ORIGIN      
        1 mligsgatls llsiglinls rdkcdlvlqk tqsgwvvagg lnneegnsvl tcnlanlseq
       61 lakfwvtena nmkssktsee slcevyyven tvrdangryv vrlpfrnsdl dlsnsrsqal
      121 rrfhfvrrrl esnpdlksey hkviqehidm ghmslvrnet angyymphha vikassattk
      181 vrgvfdasak mdkgislndt lmvdptiqps lfeqlmrfrs hvyvltadie kmyrqvlvhp
      241 edrq
//
LOCUS       XP_076545908             304 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305608 [Osmia lignaria lignaria].
ACCESSION   XP_076545908
VERSION     XP_076545908.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689793.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="uncharacterized protein LOC143305608"
                     /calculated_mol_wt=33924
     CDS             1..304
                     /gene="LOC143305608"
                     /coded_by="XM_076689793.1:1..915"
                     /db_xref="GeneID:143305608"
ORIGIN      
        1 mplarsptrv sdhhtasgsd lpvaeptrme kseststaag evnavsrvkl ppfwkenpll
       61 wfsqvetafa lsrivsddtk frnvvlvlda svlpvvadvi lsppetgkyd slrrrilesf
      121 dessesklrr llrghsmgde kpssylqrlr nlaggqcses ilktlfleqm penfrvilai
      181 segmdlgkla lqadklfeiv spslaavada hpsnvdivaa tskistdlse qlseilsrlr
      241 rleardagrd drfrrrdrks srpeerrtrs ksrdglcrlh akygadarhc fppcnwtsqg
      301 rren
//
LOCUS       XP_076545909             201 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305609 [Osmia lignaria lignaria].
ACCESSION   XP_076545909
VERSION     XP_076545909.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689794.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 79% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="uncharacterized protein LOC143305609"
                     /calculated_mol_wt=21982
     CDS             1..201
                     /gene="LOC143305609"
                     /coded_by="XM_076689794.1:1..606"
                     /db_xref="GeneID:143305609"
ORIGIN      
        1 maestnngda sagraprvpp yisgdpalwf vqletyfafs ryaddetqfr lvimnvtgdv
       61 lmavrdlatn ppatgkcpdn llrtlflerl pekvrtilav svnadlsmla eqadriceqf
      121 gvleptreac aiarppstla asselargtc aqarslqftw qksvttqggs kdglcyvhrk
      181 ygdrarhcha pcswtsrqae n
//
LOCUS       XP_076545910             338 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305610 [Osmia lignaria lignaria].
ACCESSION   XP_076545910
VERSION     XP_076545910.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689795.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 40% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="uncharacterized protein LOC143305610"
                     /calculated_mol_wt=38544
     CDS             1..338
                     /gene="LOC143305610"
                     /coded_by="XM_076689795.1:1..1017"
                     /db_xref="GeneID:143305610"
ORIGIN      
        1 msgdkkkkgs rsrsrernrk kqktderfek ienqinnltk visqlaelnk aadgknlnqs
       61 adknldkenv dtsnltqpqe aeepldiast edreipankt pinevmleml gehpdtkkei
      121 pikineelki rwqrwmqegy pedkkknile typrkddfyt eapkvnieig pvmteialkr
      181 dehfaetqnc igsaisalga avsmileepe dgidqealmk ylcdtgkllt dvfhqqskar
      241 rafitplmnk svkptvdatk adewlygkkf aeqvkeakti npksrdqgns kyppakykqv
      301 gnqqkksvir fksrpqrtny sttkassrta shrlpprk
//
LOCUS       XP_076545912             828 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger MYM-type protein 1-like [Osmia lignaria lignaria].
ACCESSION   XP_076545912
VERSION     XP_076545912.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689797.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="zinc finger MYM-type protein 1-like"
                     /calculated_mol_wt=95843
     Region          <26..141
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          161..248
                     /region_name="ZnF_TTF"
                     /note="zinc finger in transposases and transcription
                     factors; smart00597"
                     /db_xref="CDD:214739"
     Region          212..447
                     /region_name="DUF4371"
                     /note="Domain of unknown function (DUF4371); cl46273"
                     /db_xref="CDD:480613"
     Region          691..821
                     /region_name="RNase_HI_RT_Ty1"
                     /note="Ty1/Copia family of RNase HI in long-term repeat
                     retroelements; cd09272"
                     /db_xref="CDD:260004"
     Site            order(691..693,728..730,733,766,792,819)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260004"
     CDS             1..828
                     /gene="LOC143305611"
                     /coded_by="XM_076689797.1:68..2554"
                     /db_xref="GeneID:143305611"
ORIGIN      
        1 masrdrdrgr kyisgaeklk rkkqrdgyik qqtnnimkyv iettghsrqp nedeehlamy
       61 qipleegkqq lntsvkeeqe etnetemden knnrttsdce threkdiedm eieeqkeenr
      121 nfdtedpstw piertqqtvn livqhgpvqv nqfnfpknkn krsfsatyft rilangqrka
      181 rrwliysksk dkvycfackl fsitdvslgt dgfcdwshls qrikmheqsa vhlqainkwl
      241 qteqnfrtgn lidkefekll kletarceqv fqritaiily laqhnlafrg ssdtldtphn
      301 gnflgliqlv akfdsvlndh lnrtakkghh ylshsiqnel isamankvqr viidaikrak
      361 yysilldctp dmnhqeqlsv vlryvnitdd dvdieehfik feivhdttgk glsetiilil
      421 dqlgikisdc rgqgydngan mrgiyqgvqa nilrenhkaf yvpcgshnln lllgdiakss
      481 tqalnffgvl hkiytifvas tsrwdvlkkh vekvtpkpls etrwecrfds vravrfqvrq
      541 frnalneian nasdnklkie ilstvrkine fefiasliiw yeilskiniv skvlqnqkmq
      601 idlatkhlkg lnvflaeyrn agfseamita nevakeldia pdfkssyrgk krklfdyegq
      661 desstisdee yfrihyffki idtalvsfkn sdwggdvqdr kstsgyltkv fgntvrwvir
      721 kqrsvalsst eaelvalcya vqegmwikrl lkdlnvivds mtmyednqgc ialirnpenn
      781 rrvkhidlky nfiheevknn tikieylnsk rqqadiltkg lqvsnfvn
//
LOCUS       XP_076545913            1245 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305612 [Osmia lignaria lignaria].
ACCESSION   XP_076545913
VERSION     XP_076545913.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689798.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="uncharacterized protein LOC143305612"
                     /calculated_mol_wt=141484
     Region          <67..162
                     /region_name="RNase_H_like"
                     /note="Ribonuclease H-like superfamily, including RNase H,
                     HI, HII, HIII, and RNase-like domain IV of spliceosomal
                     protein Prp8; cl14782"
                     /db_xref="CDD:449355"
     Region          462..715
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(545..550,604..605,634,636..637,711..712)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(545..550,604,636)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            605
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          937..1082
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(940..943,975..977,980,1005,1061,1079)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..1245
                     /gene="LOC143305612"
                     /coded_by="XM_076689798.1:1..3738"
                     /db_xref="GeneID:143305612"
ORIGIN      
        1 mgyrittpin vmnteagimd lefraevlle rfvmkhrikg rgvvyealar tlvvrekeps
       61 eewdpwavkk arlnwgrerv liltdsasal kklegfarrt gdnpwiqeii eeimileeey
      121 kgnegveqgs ripgnrlgfa wipshvgieg ngradmkage atngeevnrv nemmgstrkf
      181 kidkinwdsf vtasgeeara lmenwnaegw enfniddrcr dlteaiikii rkaggsvtge
      241 gsnkkdvrgk akpknvkgqm wwdndcneak kdrrkavklf irtpsqanwi nlrevgkrmk
      301 klieekksra wddfassidc ytssreawqk ikqikngigk tgcanlneke insleeremg
      361 kifaydlegt qdkegqgele tpvqirerey vsqgqagiyd gfeerdlqgv ldsvrassap
      421 gedgisylll rkldsgmkkc llklynwiwe nscipqmwkr viikflekpg kkalrpisli
      481 ncmskilerl vkerlcewvg kegivdhrqs gfmkgrstmd nlvalvtdik agfkarketv
      541 aafmdvksay dlvnhnklka llkerncper vagfivnwlk drsatcvrkf legvnglikr
      601 glpqgsvlsp llyniytaei ckgmerwgvr vllyaddivv yitgedidel reklegavga
      661 llenlkelgl glaegkskvv vfskardrnr gfgefrvgee rlegeaqapf lgitldrals
      721 fskhielvsn kvrkkldilr fitgikkgvk pstmliffkg lirsvieygl flffwenera
      781 lnkimrlhna gvriamgyri ttpinvmnte agimdlesra eallerfvmk hrikgrgvvy
      841 ealartlvvr eedpseewdp wvrawrkaeg vaervmnnnt grevlnkrrd fvegggitve
      901 wevgkrrkee dypdeqlike ikdkiwprcd nkgvieiytd gskrgltevv gagvvtkgts
      961 gewseeswsl nrvtsvfsae tfallqavkk arlnwgrerv liltdsasal kklegfprrt
     1021 gdnpwiqeii eeimileeey kgnegvgqgs rspgnrlgfa wipshvgieg neradmkage
     1081 atngeevfey gvverdvmry imegawvrnh erakrkgevk gakyfsienn nagarkpwfd
     1141 sikgmnrgdl vrlsriranh fnlgeslfrk nlvkekgcic gaelesiehv vwecdrfies
     1201 reelnnflia kgynegadvq emliggnlgv lkrisrfldk lgkvi
//
LOCUS       XP_034190385             114 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608841, partial [Osmia lignaria
            lignaria].
ACCESSION   XP_034190385
VERSION     XP_034190385.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334494.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190385.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..114
                     /product="uncharacterized protein LOC117608841"
     CDS             1..114
                     /gene="LOC117608841"
                     /coded_by="XM_034334494.2:<1..345"
                     /db_xref="GeneID:117608841"
ORIGIN      
        1 csgkcsrscn ksqqlqqelq qqsqqqqsqp qqlqlqlkmq qlrmqinmlr qpkpyprhar
       61 ytpqpqpqly qqqaedtasr smqqrqlrrc rrelkrlqre gmprggksyr tggp
//
LOCUS       XP_076545914             725 aa            linear   INV 03-SEP-2025
DEFINITION  serine protease ea [Osmia lignaria lignaria].
ACCESSION   XP_076545914
VERSION     XP_076545914.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689799.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="serine protease ea"
                     /calculated_mol_wt=81707
     Region          48..>166
                     /region_name="Sds3"
                     /note="Sds3-like; pfam08598"
                     /db_xref="CDD:430099"
     Region          372..424
                     /region_name="CLIP"
                     /note="Regulatory CLIP domain of proteinases; pfam12032"
                     /db_xref="CDD:463440"
     Region          467..719
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            467
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(511,578,670)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(664,694,696)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..725
                     /gene="LOC117608791"
                     /coded_by="XM_076689799.1:281..2458"
                     /db_xref="GeneID:117608791"
ORIGIN      
        1 msyqdspfst mygqddydid edndeyledd rdaeeqdesd edteeasetd lgkseeytei
       61 keqvyqdkla slkkqlqqlk dgthpeynrk lkrleaqyke rlrlniiyrd yltewverdy
      121 ilekktavke feekkidlke nlltsmeekr kmiesdrhtl eltgdsmevk pvmtrklrrr
      181 pndpvpekie krrkpppaql nylldekeie sdlkaisrgk vltavrkpav lphyntvnmp
      241 sqhipptsdt amietriedg kllherrwfh rgqpvyvegk dltrfaanis aigteaiwvk
      301 kvsdgskvri yisqlsrgki sikrrsllpq rsvslaalgn premiklsil ctfyhvlvlg
      361 lvfaqnegqs nctsphgvvg wciglrqcpq lltilqtspl kpeaieflrq sqcgfdgndp
      421 rvccpiqnen pdlsggadga kdanlqfdls nntllpsdcg rdlsqrivgg ertdidefpw
      481 malliystpn gerascggal inhryvltaa hclrgknipi swslakvrlg eydtitnpdc
      541 ikddenskvc addaitvgie kqiahenyrp lsrdqkydia llrlnrdipf tnyikpiclp
      601 sndavgnklf vagwgkteng fssniklkvs vplvskeqce qtystrrvtl gfgqicaggv
      661 idkdsctgds ggplmcvekg sdgtgrwtav givsfgptpc gfpgwpvvyt rvidfipwil
      721 sklea
//
LOCUS       XP_076545915            1180 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608989 [Osmia lignaria lignaria].
ACCESSION   XP_076545915
VERSION     XP_076545915.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689800.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1180
                     /product="uncharacterized protein LOC117608989"
                     /calculated_mol_wt=131899
     Region          77..167
                     /region_name="Nuc_deoxyri_tr2"
                     /note="Nucleoside 2-deoxyribosyltransferase like;
                     pfam15891"
                     /db_xref="CDD:464926"
     Region          <652..800
                     /region_name="ArsB_NhaD_permease"
                     /note="Anion permease ArsB/NhaD. These permeases have been
                     shown to translocate sodium, arsenate, antimonite, sulfate
                     and organic anions across biological membranes in all
                     three kingdoms of life. A typical anion permease contains
                     8-13 transmembrane helices...; cl21473"
                     /db_xref="CDD:473875"
     Region          730..1168
                     /region_name="CitT"
                     /note="Di- and tricarboxylate antiporter [Carbohydrate
                     transport and metabolism]; COG0471"
                     /db_xref="CDD:440239"
     CDS             1..1180
                     /gene="LOC117608989"
                     /coded_by="XM_076689800.1:933..4475"
                     /db_xref="GeneID:117608989"
ORIGIN      
        1 mlslvnwtqt ykewrlaedc tiigfhayim lqefyifiie lallcnkgta vsmsasrplh
       61 cdgkdnnsks gsnrmayevf lggscnpttw rseiaiptlq slgityynpq vsqwgpelia
      121 qeyeakqtak vllfvidnqt rnsagiieaa qlaatrresl ivviypyrqg qtilgetvsi
      181 heyydlmngl lvlqylmerq ripifesvsv alnctskvlr eeinvqdlht ddgirsikis
      241 fgqngmdtvt lreifksmdt ndsgsinlee awialqssvk cnvsvsdlln vvnksetyrt
      301 liedgyrtkr dptqlrinfe qfcalaaess wrtksngvsc eseipsvwsi lcrkaskflr
      361 raivqpfnrf ldwtnslvae sekrdlyvgv igkdqfwlet naapsiesmg ltlyrsssan
      421 eynvkvlpqe lqkmknsrli llivpqhsrg itimalaahl iglraklvlc vqtlpdgciv
      481 sgeklteqar kdynrgrmyl sdyatregvp vfqniaealq haiqlvqrir wrrarwtgqq
      541 ekdkveeeqk eeakietkke rerakergdr litrgptpcv pfretnewcr rvrasvksgp
      601 rfgkglpirr gemssrqpdd pypeiieppg rrradsfgkl lvrfvsiywk slmivlwpli
      661 llplviveat eaiamrclyv vgimaifwmt evlplpvtgl ipivlyplmg imstgdtcac
      721 ymndttmmfi gsmviaivie nsglhmrval liiktigcsh rrltlglffv tmflsmwisn
      781 taatammlpi vetvllelea qglgdmfvse envedntepv kkptnvtmvy ylvaayassl
      841 ggigtlvgsg tnltlkgffe krfpdsagis ltswmlysvp pmllmsfltw lwlqvmymgl
      901 frpkskdala idigsqgerv aaavidkkyk elgsvtwhet tvgvlfvtvv llwffrspgf
      961 vrgwptyitn lqvkdstaav cvsilffilp skldflrsfd sdpanrptkc spamitwkmi
     1021 hqkmhwslil vlgggfaisa gsttsrlssi ignaliglqs inplailfiv cafaetatel
     1081 tsnvavanii lpvlaemcva lrlhplylml patlccsfsf hlpvgtppna ivtaaghikt
     1141 rdfaiagigp svitlivtal afstwgtfif nlsefpgwat
//
LOCUS       XP_034190691             239 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608986 [Osmia lignaria lignaria].
ACCESSION   XP_034190691
VERSION     XP_034190691.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334800.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="uncharacterized protein LOC117608986"
                     /calculated_mol_wt=27519
     CDS             1..239
                     /gene="LOC117608986"
                     /coded_by="XM_034334800.2:845..1564"
                     /db_xref="GeneID:117608986"
ORIGIN      
        1 mtvvyshicd rfqetannff spvkddgfmm vrskgnvkvr lpyrectmel rsmtkrpspy
       61 thmnnlnfmn lhddstirrk qgpnkvrveg edsppiiirs gcamfprfrt fgetctytmq
      121 kfrcqilnws stncgphgnr clytlkepkp dennviravh fapnlktmet mriafeeinn
      181 tctatgests newfrvfddg tnycdlhnlv tetgfdkssk flemtnnaic tqytmaicq
//
LOCUS       XP_034185221             650 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606630 [Osmia lignaria lignaria].
ACCESSION   XP_034185221
VERSION     XP_034185221.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329330.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185221.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..650
                     /product="uncharacterized protein LOC117606630"
                     /calculated_mol_wt=71218
     Region          43..299
                     /region_name="SGL"
                     /note="SMP-30/Gluconolactonase/LRE-like region; pfam08450"
                     /db_xref="CDD:462480"
     Region          351..610
                     /region_name="SGL"
                     /note="SMP-30/Gluconolactonase/LRE-like region; pfam08450"
                     /db_xref="CDD:462480"
     CDS             1..650
                     /gene="LOC117606630"
                     /coded_by="XM_034329330.2:72..2024"
                     /db_xref="GeneID:117606630"
ORIGIN      
        1 mqniicnlls fglffaafvh grslidkkke liiepvvgpf khsegphwdh htqklyfvdi
       61 vaqkicrfsp iskhvaciyi dkgpvgvavp vegepdklva gvatkfvlvn wdgqknyiks
      121 pletlavvdt drngtrwndg kvdssgrfwg gtigpevndv vvpeqaafyr idselkpkke
      181 ltvtnsngla wnlqddtlyy idtptrqvaa fdfepisgti snksivfdlq khnisglpdg
      241 mtidtngnlw ialyngggvi nvdprngkvi rfvklpvskv tsamfggpnl dvlyvttssr
      301 glnleekikq pyagyvfavh glgvhgftan sfklwkmsqv tvkplagpye lgegphwehv
      361 sqklyfvdif aqkiyrfdpv tqtitsafle ngpvsfvipv agttnkflss tgtdivllsw
      421 dgeknltkca tqtlctadss rtetrwndgk tdaagrfwgg tmgfakdgvf panvgslyvm
      481 nnnlalkkev tpvtisngla wssnndtmyy idsptrqvva ydynfqtgai snkrvvfdlq
      541 knnisgipdg mtidtdgnlw valfggggvi qinpktsell rfikingakn itsvafggpe
      601 fdtlyvtsat ielkenelke qpyagylfsi tglgvrgspa ssfklphklt
//
LOCUS       XP_076545916             576 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606366 [Osmia lignaria lignaria].
ACCESSION   XP_076545916
VERSION     XP_076545916.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689801.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 37% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..576
                     /product="uncharacterized protein LOC117606366"
                     /calculated_mol_wt=61027
     Region          <168..570
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..576
                     /gene="LOC117606366"
                     /coded_by="XM_076689801.1:26..1756"
                     /db_xref="GeneID:117606366"
ORIGIN      
        1 mkvavyqrhe dlgkhlrlhh hgasrrwvcg vcfftddsay alkkvrkhha dshannavpr
       61 aagartsapa grvsggapsv ggdsaeerps idvalpettq rpspspleaa apttrggala
      121 rakktttstn srttvaakps napkrkpvit stvtgrnllt gwaikktaat sttrksgtps
      181 spadgrppst gspatpptvd lttppagtga akrtsggsvs mrrpsggast tltppikeke
      241 kspgtkrrst ratteppqpg ppqrtaarkv tpavtyaevt rgtstvtttm ttrrmarats
      301 lppvprndkd ggasttqapp tmpvtatctf atagtltltt tsvyskpvav vtvatrrsgg
      361 gisprglkek iqedtvlles apttraratr attepprtpp rakqtkkrrs ppadmspatv
      421 sdtatpttpe erpatrrstr aktlspgstq ekrrrdgttt lpspagrpgr rrilpaiaea
      481 spvvtaapik ekgkkkekeq edeekgewrd ftprirrrrv grreeegett npsprtqarq
      541 pprlhdegae eevvrvprvv evsvapeqgp psdkvk
//
LOCUS       XP_034184890             515 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 26 [Osmia lignaria lignaria].
ACCESSION   XP_034184890
VERSION     XP_034184890.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328999.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184890.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="transmembrane protein 26"
                     /calculated_mol_wt=59938
     Region          4..299
                     /region_name="Tmem26"
                     /note="Transmembrane protein 26; pfam09772"
                     /db_xref="CDD:430813"
     CDS             1..515
                     /gene="LOC117606497"
                     /coded_by="XM_034328999.2:1..1548"
                     /db_xref="GeneID:117606497"
ORIGIN      
        1 makflatika iitrlvfaah gfiaiwqvtt fkknplfwyl scpilllffe giftlsiken
       61 qewkwfcpsv flylssvvpa iwlleldkvd rrlrafeanv nisenfdltn laaedlkhle
      121 kafgvniylp diristetwv tlieqflmli liigrwmlpk gdltrdqlsq lllvyigtaa
      181 diieffdsfk edriarepvl vyltlgiwaw slmqftvvlt atksrksrls sgsavkkrvh
      241 tetsccsidv wgialnmllq dgpflafrli liihyhivsy mnifftcknt lvillqlyrl
      301 yvvqtenrnk rkkkpetsni siisrgdmyk hvkgrkvsve rrrkrrrdnd veanysqsee
      361 tteeddqfds sprntrpkrk trqdidysts ssgkqgklkr ieikkkssrk ndskreiste
      421 eekrrrkvdr kpstkgdrnf sestrlkskq ddssksssrs rkckdnseee stteevagkt
      481 vteesesepe rkryinystl iyqqsnlnil fkili
//
LOCUS       XP_034185214             464 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606624 [Osmia lignaria lignaria].
ACCESSION   XP_034185214
VERSION     XP_034185214.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329323.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185214.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="uncharacterized protein LOC117606624"
                     /calculated_mol_wt=53165
     Region          337..387
                     /region_name="DD_R_PKA_DPY30-like"
                     /note="dimerization/docking (D/D) domains found in the
                     cAMP-dependent protein kinase regulatory subunit (PRKAR)
                     and the DPY30/SDC1-like family; cl02594"
                     /db_xref="CDD:445844"
     CDS             1..464
                     /gene="LOC117606624"
                     /coded_by="XM_034329323.2:132..1526"
                     /db_xref="GeneID:117606624"
ORIGIN      
        1 mkqiattvhe ginkyskreg tdsiislsts sstssslpsr etlnrssldl iltnrgvqly
       61 gtwsktkllt fltdrlkqci hvhrnfpyda innerlqmti ssglystsvl kkleengmsm
      121 ysilkamklg lislncceln cfknfnvvsy ilefkemwsk kfndelilnl kpmgftesec
      181 wniivhgimf qnlnilyrlt lsgidvpgil nwhsvinlts peiiqflkhi giakdiiqlv
      241 kkygiteete qmlidigfde mkeqlsnmee vvcdykltvm esstsslekl qdtckttdlk
      301 stssstdtsn yfqqyaclch llaenktvae ltaqrndylr qnimvplswa maktlefkpt
      361 dplhytayql lrwahnvset eknalqqlia latiemdrkl tekirleeee elirnlnekn
      421 tkntvckdsk qfekyarksl kkyetcefpe mcssckinvs ntsv
//
LOCUS       XP_076545917             381 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606706 [Osmia lignaria lignaria].
ACCESSION   XP_076545917
VERSION     XP_076545917.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689802.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..381
                     /product="uncharacterized protein LOC117606706"
                     /calculated_mol_wt=41524
     CDS             1..381
                     /gene="LOC117606706"
                     /coded_by="XM_076689802.1:35..1180"
                     /db_xref="GeneID:117606706"
ORIGIN      
        1 mrllivlatl atlavaeiye drqyyqyrgp paplnndgmv mdtpevahar aahlamhaet
       61 vdrlrkaqnd yemyennems ylsppmmdep mmpkrqrqrm fppsahderm mgtpemaepk
      121 nsyltthara askasefyef dvfdgqknlv yvtpvrvayk hapsvgyrgp fapigpdgrv
      181 vdtpevmrar eahmkahara iamstqndly iiltafcala ladpgylvay shlgyplayd
      241 grvldtpeva qakaahlatq ayeaarntlg yvhvpalfrv yphaitsgap igadgrvvdt
      301 pevaeakaah ltahaletak klglhpygal aytsvpyayg fgygaplgpd grvvdtpeva
      361 kakaahlaah aqeaaknagk f
//
LOCUS       XP_034185150             461 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606591 [Osmia lignaria lignaria].
ACCESSION   XP_034185150
VERSION     XP_034185150.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329259.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185150.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..461
                     /product="uncharacterized protein LOC117606591"
                     /calculated_mol_wt=50961
     CDS             1..461
                     /gene="LOC117606591"
                     /coded_by="XM_034329259.2:1..1386"
                     /db_xref="GeneID:117606591"
ORIGIN      
        1 mhlnacflcl flcvalieag papktiekks laedvkaesk kadlkieeag edkdrskkst
       61 fcvqigseas qpaqvswken qmgvqnipma lhtqsqpiqt lnlqpvaqtl pqasvmmpqq
      121 mvqppihtlq ivqsppqpca papapavnii qsvpqtkvik tiqkpkpkpk ptgeeqkpmr
      181 ieqipeplpe pqetltvlpv aptchehlml vsepeqpqma tyvqvpsmvp ctnpmhsfln
      241 pcscqqnlam fsesgiepma mkmlpiayss tyarpplimp ydtnafshvv vndnvdvkvg
      301 pqarthnhik vkyphhihvr pelsqqtiyq nmyvqgsegg ipssyasrdl vdnayatkgv
      361 tinafpqtfr dansmhqagy qfvedslgnq dnasnnptfs eaddlmqsnk aprqiesnql
      421 lpentvetkq ddgramlidg rrnarsnkek tkeiekkskt n
//
LOCUS       XP_034190502             897 aa            linear   INV 03-SEP-2025
DEFINITION  bromodomain-containing protein 140 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190502
VERSION     XP_034190502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..897
                     /product="bromodomain-containing protein 140 isoform X1"
                     /calculated_mol_wt=100719
     Region          52..590
                     /region_name="COG5141"
                     /note="PHD zinc finger-containing protein [General
                     function prediction only]"
                     /db_xref="CDD:227470"
     Region          <557..628
                     /region_name="Bromodomain"
                     /note="Bromodomains are found in many chromatin-associated
                     proteins and in nuclear histone acetyltransferases. They
                     interact specifically with acetylated lysine; cl02556"
                     /db_xref="CDD:445827"
     Region          772..882
                     /region_name="PWWP_BRPF"
                     /note="PWWP domain found in the bromodomain and PHD
                     finger-containing (BRPF) protein family; cd05839"
                     /db_xref="CDD:438964"
     Site            order(785,788,807..809,830..831,833,835..839)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438964"
     Site            786..789
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438964"
     CDS             1..897
                     /gene="Br140"
                     /coded_by="XM_034334611.2:360..3053"
                     /db_xref="GeneID:117608888"
ORIGIN      
        1 mntpqsnkkk grsriginne snspantetl ssqesskfvl vnpigedank tfkigitdsi
       61 piislegnsf dekttqkghn ipsdkdkeek nilsslpvam vkeidgyeeq lgeaeplpns
      121 yirfmersge eldgeveydl deedtawlsi vnerrlasgl npplepdtfe llmdrlekes
      181 yfqqqsnggg giaadedavc cicmdgecqn snailfcdmc nlavhqdcyg vpyipegqwl
      241 crrclqspsr avdcvlcpnr ggafkqtdrp atwahvvcal wipevrfant vflepidsie
      301 sipaarwrlt ccvckrrgsg aciqcyksnc yaafhvtcaq qaglcmrmrt vqptngepml
      361 vqktayceah tpsdyqpstn padarrraia nkksssapvi siptipperi keiaslaegl
      421 pkrsqliqrl iaywtlkrqy rngvpllrrl qsshpqsrpp tlgdnsspap dselrgelyr
      481 qlkywqclrq dlerarllce lvrkreklkk elfkvkekcl wfelrplesv lrtlleaikl
      541 kdvndvfgqp vntkevpdyl eivshpmdls tmqakierqe ydtigafead fnlmvsncla
      601 ynrkdtmfyr agtkmkeqgg alieqarkdy peldpvnese qtgvvksrkr dranrsrgea
      661 esqsgekeig gggvnrrtav lftrkarera srsnqtfald ddkkkqsdsf kvyrsgtmds
      721 dvgegesqss scsscstsrs ttpdleeekq rqeadeakke leskqeaasn glealqlvwa
      781 kcrgypwypa liidpntprg tvhkgvpipa ppddvlalav nykepvflvl ffdtkrtwqw
      841 lpgekleklg vsqeldeakl iesrkpadrk avkkayqeal hyrkqthnts lnagsas
//
LOCUS       XP_034190504             798 aa            linear   INV 03-SEP-2025
DEFINITION  bromodomain-containing protein 140 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190504
VERSION     XP_034190504.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334613.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..798
                     /product="bromodomain-containing protein 140 isoform X2"
                     /calculated_mol_wt=89979
     Region          37..491
                     /region_name="COG5141"
                     /note="PHD zinc finger-containing protein [General
                     function prediction only]"
                     /db_xref="CDD:227470"
     Region          <458..529
                     /region_name="Bromodomain"
                     /note="Bromodomains are found in many chromatin-associated
                     proteins and in nuclear histone acetyltransferases. They
                     interact specifically with acetylated lysine; cl02556"
                     /db_xref="CDD:445827"
     Region          673..783
                     /region_name="PWWP_BRPF"
                     /note="PWWP domain found in the bromodomain and PHD
                     finger-containing (BRPF) protein family; cd05839"
                     /db_xref="CDD:438964"
     Site            order(686,689,708..710,731..732,734,736..740)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438964"
     Site            687..690
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438964"
     CDS             1..798
                     /gene="Br140"
                     /coded_by="XM_034334613.2:481..2877"
                     /db_xref="GeneID:117608888"
ORIGIN      
        1 mvkeidgyee qlgeaeplpn syirfmersg eeldgeveyd ldeedtawls ivnerrlasg
       61 lnpplepdtf ellmdrleke syfqqqsngg ggiaadedav ccicmdgecq nsnailfcdm
      121 cnlavhqdcy gvpyipegqw lcrrclqsps ravdcvlcpn rggafkqtdr patwahvvca
      181 lwipevrfan tvflepidsi esipaarwrl tccvckrrgs gaciqcyksn cyaafhvtca
      241 qqaglcmrmr tvqptngepm lvqktaycea htpsdyqpst npadarrrai ankksssapv
      301 isiptipper ikeiaslaeg lpkrsqliqr liaywtlkrq yrngvpllrr lqsshpqsrp
      361 ptlgdnsspa pdselrgely rqlkywqclr qdlerarllc elvrkreklk kelfkvkekc
      421 lwfelrples vlrtlleaik lkdvndvfgq pvntkevpdy leivshpmdl stmqakierq
      481 eydtigafea dfnlmvsncl aynrkdtmfy ragtkmkeqg galieqarkd ypeldpvnes
      541 eqtgvvksrk rdranrsrge aesqsgekei ggggvnrrta vlftrkarer asrsnqtfal
      601 dddkkkqsds fkvyrsgtmd sdvgegesqs sscsscstsr sttpdleeek qrqeadeakk
      661 eleskqeaas nglealqlvw akcrgypwyp aliidpntpr gtvhkgvpip appddvlala
      721 vnykepvflv lffdtkrtwq wlpgeklekl gvsqeldeak liesrkpadr kavkkayqea
      781 lhyrkqthnt slnagsas
//
LOCUS       XP_076545918             771 aa            linear   INV 03-SEP-2025
DEFINITION  bromodomain-containing protein 140 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545918
VERSION     XP_076545918.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689803.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..771
                     /product="bromodomain-containing protein 140 isoform X3"
                     /calculated_mol_wt=87017
     Region          3..464
                     /region_name="COG5141"
                     /note="PHD zinc finger-containing protein [General
                     function prediction only]"
                     /db_xref="CDD:227470"
     Region          <431..502
                     /region_name="Bromodomain"
                     /note="Bromodomains are found in many chromatin-associated
                     proteins and in nuclear histone acetyltransferases. They
                     interact specifically with acetylated lysine; cl02556"
                     /db_xref="CDD:445827"
     Region          646..756
                     /region_name="PWWP_BRPF"
                     /note="PWWP domain found in the bromodomain and PHD
                     finger-containing (BRPF) protein family; cd05839"
                     /db_xref="CDD:438964"
     Site            order(659,662,681..683,704..705,707,709..713)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438964"
     Site            660..663
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438964"
     CDS             1..771
                     /gene="Br140"
                     /coded_by="XM_076689803.1:108..2423"
                     /db_xref="GeneID:117608888"
ORIGIN      
        1 mvynfikrev eydldeedta wlsivnerrl asglnpplep dtfellmdrl ekesyfqqqs
       61 nggggiaade davccicmdg ecqnsnailf cdmcnlavhq dcygvpyipe gqwlcrrclq
      121 spsravdcvl cpnrggafkq tdrpatwahv vcalwipevr fantvflepi dsiesipaar
      181 wrltccvckr rgsgaciqcy ksncyaafhv tcaqqaglcm rmrtvqptng epmlvqktay
      241 ceahtpsdyq pstnpadarr raiankksss apvisiptip perikeiasl aeglpkrsql
      301 iqrliaywtl krqyrngvpl lrrlqsshpq srpptlgdns spapdselrg elyrqlkywq
      361 clrqdlerar llcelvrkre klkkelfkvk ekclwfelrp lesvlrtlle aiklkdvndv
      421 fgqpvntkev pdyleivshp mdlstmqaki erqeydtiga feadfnlmvs nclaynrkdt
      481 mfyragtkmk eqggalieqa rkdypeldpv neseqtgvvk srkrdranrs rgeaesqsge
      541 keiggggvnr rtavlftrka rerasrsnqt faldddkkkq sdsfkvyrsg tmdsdvgege
      601 sqssscsscs tsrsttpdle eekqrqeade akkeleskqe aasnglealq lvwakcrgyp
      661 wypaliidpn tprgtvhkgv pipappddvl alavnykepv flvlffdtkr twqwlpgekl
      721 eklgvsqeld eakliesrkp adrkavkkay qealhyrkqt hntslnagsa s
//
LOCUS       XP_034190505             842 aa            linear   INV 03-SEP-2025
DEFINITION  adenomatous polyposis coli protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190505
VERSION     XP_034190505.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334614.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190505.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..842
                     /product="adenomatous polyposis coli protein isoform X1"
                     /calculated_mol_wt=92882
     Region          101..128
                     /region_name="APC_r"
                     /note="APC repeat; pfam05923"
                     /db_xref="CDD:461781"
     Region          399..422
                     /region_name="APC_r"
                     /note="APC repeat; pfam05923"
                     /db_xref="CDD:461781"
     CDS             1..842
                     /gene="LOC117608889"
                     /coded_by="XM_034334614.2:165..2693"
                     /db_xref="GeneID:117608889"
ORIGIN      
        1 merstgrrye detteqpvdy skkyrerktg genhsienst rtvrsckkey rerdskvdlf
       61 gdyaetdldq ptdyslryae ddtdddekqs peyfpgsvqe dtiktycteg tpyetpfnfs
      121 tatsmsdlrl edtkesvdsq kkhnkkvhdh grkedlcfer alslncneeq sllvekelkt
      181 sktsqtrkps cpspekliny yqagtpdgfs ranslnslss attqrnnvtg iiskvsstds
      241 sekadnvsdr aersgiasnm nglrisdetd spiegksntr vvdkegkmvt fsrqdyyaeq
      301 tplmfsrcss lgslsgfeqh sihddrssvi sdfsrrtsgv vspselpdsp tqtvplsprd
      361 hksqctdfis kipeeavrps vrhlsyskcp vprgsvfedd iatfkeestp iefstatsls
      421 sltiddelki tnsskmendl kknsndlgkd vketssveki nnvetekdqe qvsdgdedde
      481 dmlaacismg iqnnryrqsf ktstvqksvq pessnmlgrc qrapalnrle ssipvtsvdt
      541 pataaktnkt gievvaapdt vhvyctedtp adispvgsqs nlsalsmpsv qedvekieea
      601 kpssevecqr ndlsdessnl sgedekilhe ciqsgipkvr qitppltsci pfaqkteavs
      661 qrfnicgtps sspvesgnrn palrkplcrt slendnelsd dspnhsddea ilseciraam
      721 pkvrlnissl isqslpqtmd kskvtnsvkv ssstasyrhp eykekkhqpk kpvtfednls
      781 lseeeediil aqciksgmpk alngslnssl thgrrvagta srgvasgsfl tptalssera
      841 pi
//
LOCUS       XP_034190506             826 aa            linear   INV 03-SEP-2025
DEFINITION  adenomatous polyposis coli protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190506
VERSION     XP_034190506.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334615.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190506.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..826
                     /product="adenomatous polyposis coli protein isoform X2"
                     /calculated_mol_wt=91128
     Region          101..128
                     /region_name="APC_r"
                     /note="APC repeat; pfam05923"
                     /db_xref="CDD:461781"
     Region          399..422
                     /region_name="APC_r"
                     /note="APC repeat; pfam05923"
                     /db_xref="CDD:461781"
     CDS             1..826
                     /gene="LOC117608889"
                     /coded_by="XM_034334615.2:185..2665"
                     /db_xref="GeneID:117608889"
ORIGIN      
        1 merstgrrye detteqpvdy skkyrerktg genhsienst rtvrsckkey rerdskvdlf
       61 gdyaetdldq ptdyslryae ddtdddekqs peyfpgsvqe dtiktycteg tpyetpfnfs
      121 tatsmsdlrl edtkesvdsq kkhnkkvhdh grkedlcfer alslncneeq sllvekelkt
      181 sktsqtrkps cpspekliny yqagtpdgfs ranslnslss attqrnnvtg iiskvsstds
      241 sekadnvsdr aersgiasnm nglrisdetd spiegksntr vvdkegkmvt fsrqdyyaeq
      301 tplmfsrcss lgslsgfeqh sihddrssvi sdfsrrtsgv vspselpdsp tqtvplsprd
      361 hksqctdfis kipeeavrps vrhlsyskcp vprgsvfedd iatfkeestp iefstatsls
      421 sltiddelki tnsskmendl kknsndlgkd vketssveki nnvetekdqe qvsdgdedde
      481 dmlaacismg iqnnryrqsf ktstvqksvq pessnmlgrc qrapalnrle ssipvtsvdt
      541 pataaktnkt gievvaapdt vhvyctedtp adispvgsqs nlsalsmpsv qedvekieea
      601 kpssevecqr ndlsdessnl sgedekilhe ciqsgipkkt eavsqrfnic gtpssspves
      661 gnrnpalrkp lcrtslendn elsddspnhs ddeailseci raampkvrln isslisqslp
      721 qtmdkskvtn svkvssstas yrhpeykekk hqpkkpvtfe dnlslseeee diilaqciks
      781 gmpkalngsl nsslthgrrv agtasrgvas gsfltptals serapi
//
LOCUS       XP_034190364             716 aa            linear   INV 03-SEP-2025
DEFINITION  mast/stem cell growth factor receptor kita [Osmia lignaria
            lignaria].
ACCESSION   XP_034190364
VERSION     XP_034190364.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334473.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..716
                     /product="mast/stem cell growth factor receptor kita"
                     /calculated_mol_wt=81154
     Region          372..690
                     /region_name="PTKc"
                     /note="Catalytic domain of Protein Tyrosine Kinases;
                     cd00192"
                     /db_xref="CDD:270623"
     Site            order(374..378,382,396,398,449..452,455..456,557,561..562,
                     564,575,593..597,606,640)
                     /site_type="active"
                     /db_xref="CDD:270623"
     Site            order(374..375,377..378,382,396,398,449..452,455..456,
                     561..562,564,575)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270623"
     Site            order(557,561,593..597,606,640)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270623"
     Site            order(574..581,593..599)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270623"
     CDS             1..716
                     /gene="LOC117608831"
                     /coded_by="XM_034334473.2:1..2151"
                     /db_xref="GeneID:117608831"
ORIGIN      
        1 mntrirfsfa cvifislylc deayrkidfv epvrnlsvka vqderytnsr daykylklni
       61 swlpansnrs psyysiiitt vpnmekpnae cpegssfytv hnnkqfnvll petneledlp
      121 dvhiqpgcsy kievianprt khminipqvl ytvpecigdt cscfiakspv pkvnviqkgy
      181 rmvvnwtapn asdvsfylis igvsrstskk glpiynnvtk igevpagttt flwnlsstek
      241 ytkvkggykv iinavnnhdc igrggsfiin stlfnvktni mnhnvwiilc gvlsgcilfg
      301 vlslilypkh dflsfgnktn arirriyryk shwaeailqk dnilyglygs egvhkeetdk
      361 lqvpfknikl irelgagqfg kvylgclndk nntliavkms qenilspkle iqqqflkeie
      421 imrmagthpn lvdllgyciq pngsicille ymqggdllty lhtfqgkeks dkpmqvneiv
      481 lerllkpnvs etlyasicpi vpnnmnankl gqytnitgse eeehwigqme eyqflkfatd
      541 iaagmehlel kgithrdlaa rnillsadlt vkisdfglsr ngiymirnie eksrrlpirw
      601 mspeairdrt fssksdvwsy givlweistl gafpysnvqd drvlryiihe ngrlekpddv
      661 plciynlmcs cwaaepedrp nftqllselk tttaslnslr sisnpcyals finent
//
LOCUS       XP_034190365             706 aa            linear   INV 03-SEP-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034190365
VERSION     XP_034190365.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334474.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190365.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..706
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=81226
     Region          49..173
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          311..705
                     /region_name="OafA"
                     /note="Peptidoglycan/LPS O-acetylase OafA/YrhL, contains
                     acyltransferase and SGNH-hydrolase domains [Cell
                     wall/membrane/envelope biogenesis]; COG1835"
                     /db_xref="CDD:441440"
     CDS             1..706
                     /gene="LOC117608833"
                     /coded_by="XM_034334474.2:757..2877"
                     /db_xref="GeneID:117608833"
ORIGIN      
        1 mkykrmkiil lactcvtina lkpepealmr nlpgyaittg tqqlnstrcr remqifrdav
       61 dngvlwslrm ldasgqptsg yisgnnywvg nrqeceflsq krqfplsekt irnnsiyrnp
      121 aeefppfeln ffvahlnqng tvqyhisvpm etiivlglcl pascseyevg tmlkkvfddr
      181 lllvgqlyst dfelmkisnl kddhawllsg kiitilfilt vlfgttivat lydiliyqkr
      241 lqkktddddd dddggtielk nntvekliyi eedavpsepk aenriakili cfsaysnmkq
      301 ifkldnssnd lkafhgmkvi gmiwimfghv iyytlnvign kaedyiilsn iesqilangt
      361 fvvdtfffis gfllvlgfpk klqrnennas fsrnvmifir aiikkyirvt pmyliiills
      421 iinftwiekf slmyfhepmh elcskywwrn ilyinnlfew ddmcltwswy lannmqffvf
      481 gifvlilsss yyytalslsi atmflcilvh ayitydigyi ptfdiqletl tsiyilpwmr
      541 ippfivgmgt avflakcnyk lhlsrkwlas cwflsvlcnc tilfgmsnrn vsltvsvfye
      601 tfsrfawslg iawlviacat nnggivnqfl slkywtpfsr vtfcayllnp fliqlmgmfs
      661 nypfnvevlt tdvygiaicv vtyfvafgls amievpttif lrtlst
//
LOCUS       XP_034190366             702 aa            linear   INV 03-SEP-2025
DEFINITION  nose resistant to fluoxetine protein 6-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034190366
VERSION     XP_034190366.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334475.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190366.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..702
                     /product="nose resistant to fluoxetine protein 6-like"
                     /calculated_mol_wt=80563
     Region          49..173
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          307..686
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..702
                     /gene="LOC117608834"
                     /coded_by="XM_034334475.2:3277..5385"
                     /db_xref="GeneID:117608834"
ORIGIN      
        1 mkykrmkiil lactcvtina lkpepealmg nspgyaittg iqnlnstrcr remqifrdav
       61 dngvlwsfrm ldasgqptsg yisgnnywvg nrqeceflsq krqfplsekk irnnsiyrnp
      121 aeefppfeln ffgahlsqng tvqyhmrvpi deiivlglcl pascseyevg tmlkkvfddr
      181 lllvgqlyst dfelmkisnl kddhawllsg kiimilfilt ilfgttivat lydiliyqkr
      241 lqkktddddd gtiefknnte ekseyneeda vpsepkaenr iakilicfsa ysnmkeifkl
      301 dnssndlkaf hgmkvigmiw liffhviyyt lnvtgnkaes aiattnielq iiangtfpvd
      361 tfffisgflv vfgftkklqk nennasfsrn vmifiravik kyirftptyl iiillaiinf
      421 twiekfslmd fnepmhelcs kywwrnilyi nnlfewndmc ltwswyiann mqffvfgtfv
      481 lilsssyyyt alslsmatmf lsmlvhayit ydigyiptld mlletftsmy irpwmrippy
      541 ivgmgtavfl akcnyklhls rkalavcwfl svlcictilf gitnrnvslt vsvlyetfsr
      601 fawslgiawl viacatnngg ivnqflslny wipfsrvtfc ayllnpfliq lmgmfsnypf
      661 nvevlttgay giaicvvtff vafglsamie vpttiflrtl tt
//
LOCUS       XP_034190370             641 aa            linear   INV 03-SEP-2025
DEFINITION  ezrin/radixin/moesin family protein merlin isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190370
VERSION     XP_034190370.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334479.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="ezrin/radixin/moesin family protein merlin
                     isoform X1"
                     /calculated_mol_wt=74698
     Region          19..218
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          212..342
                     /region_name="FERM_C_ERM"
                     /note="FERM domain C-lobe/F3 of the ERM family; cd13194"
                     /db_xref="CDD:270015"
     Site            order(254..255,257..263,272,327,330..331,334)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(254,257..264,272,327,330..331,334)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(290,328)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270015"
     Site            327..342
                     /site_type="other"
                     /note="actin binding site 2 [polypeptide binding]"
                     /db_xref="CDD:270015"
     Region          376..495
                     /region_name="ERM_helical"
                     /note="Ezrin/radixin/moesin, alpha-helical domain;
                     pfam20492"
                     /db_xref="CDD:466641"
     Region          565..641
                     /region_name="ERM_C"
                     /note="Ezrin/radixin/moesin family C terminal; pfam00769"
                     /db_xref="CDD:459932"
     CDS             1..641
                     /gene="Mer"
                     /coded_by="XM_034334479.2:293..2218"
                     /db_xref="GeneID:117608836"
ORIGIN      
        1 mkskmppfrr kksgksfpvk vctldaelef slewrstgrd lfdlvcrtig lretwyfglq
       61 yedakgfisw lkldkkvqdq cisqqpttpf mflakfypea vaeelvqevt qhlfflqvkq
      121 ailsmdiycp peasvllasy avqakygdyd evsyrpgmla sedllpqrvi dqyqmtpemw
      181 edrikiwyad hrgmsrdeae meylkiaqdl dmygvnyfpi snkketdlwl gvtalglniy
      241 ekenklapkt tftwseirhi sfddkkfvik pvektspnfv ffsqkvrmnk lvkkqsdvgs
      301 wvkglialrl ddhnsdkaah ilfvkildlc ignhdlfmrr rkpdsmevqq mkaqakeeks
      361 rrqiernkla rekqlreaae rekaameqrl lqyqeeirla nealrrseet adllaeksrv
      421 aeeeamllsq kaseaeqeit rirlnnmkte eekvhlerkt reaellterl vqeserraae
      481 aeklkdellr ariaekeake klleflsrna ytatiapvpn lfpstqvlps dlqadlqtlq
      541 ldteplptdl tsydliadgd vdqlsleiek ervdyweksk hlqeqlrelr teievmkvge
      601 kqceldqlhe eqvrlgenky stlkkvksgs tkarvaffee l
//
LOCUS       XP_034190371             641 aa            linear   INV 03-SEP-2025
DEFINITION  ezrin/radixin/moesin family protein merlin isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190371
VERSION     XP_034190371.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334480.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="ezrin/radixin/moesin family protein merlin
                     isoform X1"
                     /calculated_mol_wt=74698
     Region          19..218
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          212..342
                     /region_name="FERM_C_ERM"
                     /note="FERM domain C-lobe/F3 of the ERM family; cd13194"
                     /db_xref="CDD:270015"
     Site            order(254..255,257..263,272,327,330..331,334)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(254,257..264,272,327,330..331,334)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(290,328)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270015"
     Site            327..342
                     /site_type="other"
                     /note="actin binding site 2 [polypeptide binding]"
                     /db_xref="CDD:270015"
     Region          376..495
                     /region_name="ERM_helical"
                     /note="Ezrin/radixin/moesin, alpha-helical domain;
                     pfam20492"
                     /db_xref="CDD:466641"
     Region          565..641
                     /region_name="ERM_C"
                     /note="Ezrin/radixin/moesin family C terminal; pfam00769"
                     /db_xref="CDD:459932"
     CDS             1..641
                     /gene="Mer"
                     /coded_by="XM_034334480.2:360..2285"
                     /db_xref="GeneID:117608836"
ORIGIN      
        1 mkskmppfrr kksgksfpvk vctldaelef slewrstgrd lfdlvcrtig lretwyfglq
       61 yedakgfisw lkldkkvqdq cisqqpttpf mflakfypea vaeelvqevt qhlfflqvkq
      121 ailsmdiycp peasvllasy avqakygdyd evsyrpgmla sedllpqrvi dqyqmtpemw
      181 edrikiwyad hrgmsrdeae meylkiaqdl dmygvnyfpi snkketdlwl gvtalglniy
      241 ekenklapkt tftwseirhi sfddkkfvik pvektspnfv ffsqkvrmnk lvkkqsdvgs
      301 wvkglialrl ddhnsdkaah ilfvkildlc ignhdlfmrr rkpdsmevqq mkaqakeeks
      361 rrqiernkla rekqlreaae rekaameqrl lqyqeeirla nealrrseet adllaeksrv
      421 aeeeamllsq kaseaeqeit rirlnnmkte eekvhlerkt reaellterl vqeserraae
      481 aeklkdellr ariaekeake klleflsrna ytatiapvpn lfpstqvlps dlqadlqtlq
      541 ldteplptdl tsydliadgd vdqlsleiek ervdyweksk hlqeqlrelr teievmkvge
      601 kqceldqlhe eqvrlgenky stlkkvksgs tkarvaffee l
//
LOCUS       XP_034190372             607 aa            linear   INV 03-SEP-2025
DEFINITION  ezrin/radixin/moesin family protein merlin isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190372
VERSION     XP_034190372.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334481.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..607
                     /product="ezrin/radixin/moesin family protein merlin
                     isoform X2"
                     /calculated_mol_wt=70926
     Region          19..218
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          212..308
                     /region_name="FERM_C_ERM"
                     /note="FERM domain C-lobe/F3 of the ERM family; cd13194"
                     /db_xref="CDD:270015"
     Site            order(254..255,257..263,272,293,296..297,300)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(254,257..264,272,293,296..297,300)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270015"
     Site            order(290,294)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270015"
     Site            293..308
                     /site_type="other"
                     /note="actin binding site 2 [polypeptide binding]"
                     /db_xref="CDD:270015"
     Region          342..461
                     /region_name="ERM_helical"
                     /note="Ezrin/radixin/moesin, alpha-helical domain;
                     pfam20492"
                     /db_xref="CDD:466641"
     Region          531..607
                     /region_name="ERM_C"
                     /note="Ezrin/radixin/moesin family C terminal; pfam00769"
                     /db_xref="CDD:459932"
     CDS             1..607
                     /gene="Mer"
                     /coded_by="XM_034334481.2:291..2114"
                     /db_xref="GeneID:117608836"
ORIGIN      
        1 mkskmppfrr kksgksfpvk vctldaelef slewrstgrd lfdlvcrtig lretwyfglq
       61 yedakgfisw lkldkkvqdq cisqqpttpf mflakfypea vaeelvqevt qhlfflqvkq
      121 ailsmdiycp peasvllasy avqakygdyd evsyrpgmla sedllpqrvi dqyqmtpemw
      181 edrikiwyad hrgmsrdeae meylkiaqdl dmygvnyfpi snkketdlwl gvtalglniy
      241 ekenklapkt tftwseirhi sfddkkfvik pvektspnfv ffsqkvrmnk lildlcignh
      301 dlfmrrrkpd smevqqmkaq akeeksrrqi ernklarekq lreaaereka ameqrllqyq
      361 eeirlaneal rrseetadll aeksrvaeee amllsqkase aeqeitrirl nnmkteeekv
      421 hlerktreae llterlvqes erraaeaekl kdellraria ekeakeklle flsrnaytat
      481 iapvpnlfps tqvlpsdlqa dlqtlqldte plptdltsyd liadgdvdql sleiekervd
      541 ywekskhlqe qlrelrteie vmkvgekqce ldqlheeqvr lgenkystlk kvksgstkar
      601 vaffeel
//
LOCUS       XP_034190361             464 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional adaptor 3 [Osmia lignaria lignaria].
ACCESSION   XP_034190361
VERSION     XP_034190361.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334470.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="transcriptional adaptor 3"
                     /calculated_mol_wt=52239
     Region          324..433
                     /region_name="Ada3"
                     /note="Histone acetyltransferases subunit 3; pfam10198"
                     /db_xref="CDD:462993"
     CDS             1..464
                     /gene="Ada3"
                     /coded_by="XM_034334470.2:599..1993"
                     /db_xref="GeneID:117608829"
ORIGIN      
        1 msgkgkqssk kaivkvresg ksstlvqsst lnsdtstest ettlslpilk iadnsrllpr
       61 yssilqrtae egvgmedldt lqlelemlls svvvrhrmlq eeianlssae errdkrsksg
      121 kglslldkkv reekfkpkev naktqsplpa klfkqkaias tnsqvipnvh eisriegsks
      181 espklllpkn dtpnkfwasv dpyctdimpd diklleelia thsdvsdfkk ipplgrhysl
      241 mwahsdllqe edaanpnrdk kknrsdvsll vakndrkahs iagpltqrlv salleenvyv
      301 annnadnklf rdsdppvlrd ltiqnsinle lrmhkelveq gilepdaqkk cqeddeilae
      361 ikrcqqelta lsnhnvtqlk rllnlaqees krqalkrkis tadneviehy kklmlskqrk
      421 vpltkkeqdk awaclreren lldqlnmlph nnvgepigfg tttn
//
LOCUS       XP_034190507             393 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting-associated protein YL-1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190507
VERSION     XP_034190507.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334616.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="vacuolar protein sorting-associated protein
                     YL-1"
                     /calculated_mol_wt=45766
     Region          5..205
                     /region_name="YL1"
                     /note="YL1 nuclear protein; pfam05764"
                     /db_xref="CDD:461734"
     Region          312..340
                     /region_name="YL1_C"
                     /note="YL1 nuclear protein C-terminal domain; pfam08265"
                     /db_xref="CDD:462412"
     CDS             1..393
                     /gene="YL-1"
                     /coded_by="XM_034334616.2:352..1533"
                     /db_xref="GeneID:117608890"
ORIGIN      
        1 masnrerran agnkmaklln eeeeddfykt tyggfdeveq dhdymeedea edevdsdfsi
       61 dendepisdt eqegpkkkrr llvtkaykep kvqsqlaske kkvrqprqki fmesierksi
      121 rrstaaksaa tqrrlkerne dqkrktrtkr ydtykptqee lleealeteq invkslekyq
      181 klenekkntr tvrkaqtgpm iryqslampv mvlsevnlnk eedkinidgd dekstsenve
      241 nedtdeatqe sdvkpenevk eipkkdsstk skcryeqtkg yyertfitfe neqlfsaafk
      301 kptvqrpptk alcaitrlpa kyldpmtqlp yknvqtfrll reayyqqlea rtdindtsqs
      361 pellrwiewr qkscgggqrn tvrlepasaa mps
//
LOCUS       XP_034190508             383 aa            linear   INV 03-SEP-2025
DEFINITION  beta-ureidopropionase pyd3 [Osmia lignaria lignaria].
ACCESSION   XP_034190508
VERSION     XP_034190508.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334617.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="beta-ureidopropionase pyd3"
                     /calculated_mol_wt=43297
     Region          10..369
                     /region_name="nitrilase"
                     /note="Nitrilase superfamily, including nitrile- or
                     amide-hydrolyzing enzymes and amide-condensing enzymes;
                     cl11424"
                     /db_xref="CDD:448250"
     Site            order(117,194,198,205,231..232,234..235,256)
                     /site_type="active"
                     /db_xref="CDD:143587"
     Site            order(117,194,231)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:143587"
     Site            order(195..198,204,211..213,232,234..238,241..242,
                     268..269,271..273,275..276,313..314,356..359)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143587"
     CDS             1..383
                     /gene="pyd3"
                     /coded_by="XM_034334617.2:113..1264"
                     /db_xref="GeneID:117608891"
ORIGIN      
        1 mskpmfdqtl eeileknlpe reltevkrll ygrelqplnl pkwtgtqkfd vqgyvmgggv
       61 teqlrppriv rvgaiqnsiv lptteplqkq rnalhlkiqk yvdyaascgv nilclqemwa
      121 mpfafctrek ypwcemaeda engptiilmc dlaqrhgmvi icsvlerdta ngdilwntsv
      181 vidsdgkilg khrknhiprv gdfnestyym egntghpvfd tsfgrvaini cygrhhpqnw
      241 mmfglngaei vfnpsattsh tseplwsiea rnaaiansyy tcainrvgte tfpneftsgd
      301 gapahrdfgh fygssyitsp dgtrtpglsr ckdgllvcel dlnlcrqmkd fwclrmtqrl
      361 dlyateltdf vkknvklyek tsq
//
LOCUS       XP_034190373             379 aa            linear   INV 03-SEP-2025
DEFINITION  nonsense-mediated mRNA decay factor SMG9 [Osmia lignaria lignaria].
ACCESSION   XP_034190373
VERSION     XP_034190373.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334482.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..379
                     /product="nonsense-mediated mRNA decay factor SMG9"
                     /calculated_mol_wt=43171
     Region          106..>232
                     /region_name="Smg8_Smg9"
                     /note="pfam10220"
                     /db_xref="CDD:463005"
     CDS             1..379
                     /gene="LOC117608837"
                     /coded_by="XM_034334482.2:278..1417"
                     /db_xref="GeneID:117608837"
ORIGIN      
        1 mmsitpkdse vkevdgrgti kvidrptfil ktregenrav spsqrngsnk ketdtstnvp
       61 skvqdsvrti lpmclemttc vkfmdenmql cenpleflyd qqdflvvgcl gaqgvgksti
      121 mslltssyvp dvfpvqdish hesganctsg idffvtknrv iyldtqpils gsridysasy
      181 dqkksttdfv ntetnlelqs lqfaaflfsv chviifvqdw fidpnlvrfl qtaemlkpss
      241 tsnmdqdyve yyphivflhn kaeledftpn tiekvkefys kvfsssrlqi nsgldmsnhs
      301 meaglnlffi pritnyeept mhynekklie klktkihgvn rnpmtpstlt eknwyhyvsr
      361 vmeaikkshl sseygrlmp
//
LOCUS       XP_034190509             370 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial glutathione transporter SLC25A40 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190509
VERSION     XP_034190509.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334618.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..370
                     /product="mitochondrial glutathione transporter SLC25A40"
                     /calculated_mol_wt=41660
     Region          19..142
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          156..245
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          253..347
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..370
                     /gene="LOC117608892"
                     /coded_by="XM_034334618.2:413..1525"
                     /db_xref="GeneID:117608892"
ORIGIN      
        1 mnklsitipp nldmddprfr vrpyqqvvaa ctgafitsmf vtpldvvkir lqtqqkamls
       61 nkcflycngl mdhlcpclng rgpewarvng kfngtidalv kisrnegils lwsglsptlv
      121 lavpatiayf vsyeqlrlyf kdiynkkikh ntmggkeqpf wipmlagaia riwaatlvsp
      181 lelirtkmqs qklnyseitq alktvvkysg vfglwmglss tllrdvpfsa iywlnyetik
      241 dkfggpqqtf tfnltsgaia gsiaafltip fdvvkthrqi emgekeiysd kpgrssrtwn
      301 iinkiysqng ikglftglip rvikvapaca imiatfehgk rffvmynanq ivqydendiq
      361 llqhkrdste
//
LOCUS       XP_034190510             370 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial glutathione transporter SLC25A40 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190510
VERSION     XP_034190510.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334619.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..370
                     /product="mitochondrial glutathione transporter SLC25A40"
                     /calculated_mol_wt=41660
     Region          19..142
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          156..245
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          253..347
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..370
                     /gene="LOC117608892"
                     /coded_by="XM_034334619.2:316..1428"
                     /db_xref="GeneID:117608892"
ORIGIN      
        1 mnklsitipp nldmddprfr vrpyqqvvaa ctgafitsmf vtpldvvkir lqtqqkamls
       61 nkcflycngl mdhlcpclng rgpewarvng kfngtidalv kisrnegils lwsglsptlv
      121 lavpatiayf vsyeqlrlyf kdiynkkikh ntmggkeqpf wipmlagaia riwaatlvsp
      181 lelirtkmqs qklnyseitq alktvvkysg vfglwmglss tllrdvpfsa iywlnyetik
      241 dkfggpqqtf tfnltsgaia gsiaafltip fdvvkthrqi emgekeiysd kpgrssrtwn
      301 iinkiysqng ikglftglip rvikvapaca imiatfehgk rffvmynanq ivqydendiq
      361 llqhkrdste
//
LOCUS       XP_076545919             370 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial glutathione transporter SLC25A40 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545919
VERSION     XP_076545919.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689804.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..370
                     /product="mitochondrial glutathione transporter SLC25A40"
                     /calculated_mol_wt=41660
     Region          19..142
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          156..245
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          253..347
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..370
                     /gene="LOC117608892"
                     /coded_by="XM_076689804.1:288..1400"
                     /db_xref="GeneID:117608892"
ORIGIN      
        1 mnklsitipp nldmddprfr vrpyqqvvaa ctgafitsmf vtpldvvkir lqtqqkamls
       61 nkcflycngl mdhlcpclng rgpewarvng kfngtidalv kisrnegils lwsglsptlv
      121 lavpatiayf vsyeqlrlyf kdiynkkikh ntmggkeqpf wipmlagaia riwaatlvsp
      181 lelirtkmqs qklnyseitq alktvvkysg vfglwmglss tllrdvpfsa iywlnyetik
      241 dkfggpqqtf tfnltsgaia gsiaafltip fdvvkthrqi emgekeiysd kpgrssrtwn
      301 iinkiysqng ikglftglip rvikvapaca imiatfehgk rffvmynanq ivqydendiq
      361 llqhkrdste
//
LOCUS       XP_034190511             370 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial glutathione transporter SLC25A40 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190511
VERSION     XP_034190511.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334620.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..370
                     /product="mitochondrial glutathione transporter SLC25A40"
                     /calculated_mol_wt=41660
     Region          19..142
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          156..245
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          253..347
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..370
                     /gene="LOC117608892"
                     /coded_by="XM_034334620.2:456..1568"
                     /db_xref="GeneID:117608892"
ORIGIN      
        1 mnklsitipp nldmddprfr vrpyqqvvaa ctgafitsmf vtpldvvkir lqtqqkamls
       61 nkcflycngl mdhlcpclng rgpewarvng kfngtidalv kisrnegils lwsglsptlv
      121 lavpatiayf vsyeqlrlyf kdiynkkikh ntmggkeqpf wipmlagaia riwaatlvsp
      181 lelirtkmqs qklnyseitq alktvvkysg vfglwmglss tllrdvpfsa iywlnyetik
      241 dkfggpqqtf tfnltsgaia gsiaafltip fdvvkthrqi emgekeiysd kpgrssrtwn
      301 iinkiysqng ikglftglip rvikvapaca imiatfehgk rffvmynanq ivqydendiq
      361 llqhkrdste
//
LOCUS       XP_076545920             330 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 12 [Osmia lignaria lignaria].
ACCESSION   XP_076545920
VERSION     XP_076545920.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689805.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="peroxisomal biogenesis factor 12"
                     /calculated_mol_wt=38165
     Region          24..249
                     /region_name="Pex2_Pex12"
                     /note="Pex2 / Pex12 amino terminal region; pfam04757"
                     /db_xref="CDD:398431"
     Region          275..328
                     /region_name="mRING_PEX12"
                     /note="Modified RING finger found in peroxin-12 (PEX12)
                     and similar proteins; cd16451"
                     /db_xref="CDD:438115"
     Site            order(277,280,298,301)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:438115"
     CDS             1..330
                     /gene="Pex12"
                     /coded_by="XM_076689805.1:106..1098"
                     /db_xref="GeneID:117608838"
ORIGIN      
        1 maekgayltg taftkpsife iiaqesfast vepafkkils fivsfnhgry ehvlkwadec
       61 ylifnvilqr yylkkhsasf seafyslkri aienssnqre lskqqkgisl illvlfpylk
      121 nkffqlsqry klekidgylp qtkweklyri fivkgytvtf mlyefmvlyn yvlyisgksv
      181 ftspllrlls isltytepkm visvsdllrk iknnsfgisd gldifqrvvt tslefgaffl
      241 qflswwnqeh yftnlmslpv ppppvipeia kkykgmcpic cktprvhtvl svsgyafcyq
      301 cilsvirksg kcpvtnypak eddlirlyld
//
LOCUS       XP_034190376             330 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 12 [Osmia lignaria lignaria].
ACCESSION   XP_034190376
VERSION     XP_034190376.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="peroxisomal biogenesis factor 12"
                     /calculated_mol_wt=38165
     Region          24..249
                     /region_name="Pex2_Pex12"
                     /note="Pex2 / Pex12 amino terminal region; pfam04757"
                     /db_xref="CDD:398431"
     Region          275..328
                     /region_name="mRING_PEX12"
                     /note="Modified RING finger found in peroxin-12 (PEX12)
                     and similar proteins; cd16451"
                     /db_xref="CDD:438115"
     Site            order(277,280,298,301)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:438115"
     CDS             1..330
                     /gene="Pex12"
                     /coded_by="XM_034334485.2:165..1157"
                     /db_xref="GeneID:117608838"
ORIGIN      
        1 maekgayltg taftkpsife iiaqesfast vepafkkils fivsfnhgry ehvlkwadec
       61 ylifnvilqr yylkkhsasf seafyslkri aienssnqre lskqqkgisl illvlfpylk
      121 nkffqlsqry klekidgylp qtkweklyri fivkgytvtf mlyefmvlyn yvlyisgksv
      181 ftspllrlls isltytepkm visvsdllrk iknnsfgisd gldifqrvvt tslefgaffl
      241 qflswwnqeh yftnlmslpv ppppvipeia kkykgmcpic cktprvhtvl svsgyafcyq
      301 cilsvirksg kcpvtnypak eddlirlyld
//
LOCUS       XP_034190375             330 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 12 [Osmia lignaria lignaria].
ACCESSION   XP_034190375
VERSION     XP_034190375.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334484.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="peroxisomal biogenesis factor 12"
                     /calculated_mol_wt=38165
     Region          24..249
                     /region_name="Pex2_Pex12"
                     /note="Pex2 / Pex12 amino terminal region; pfam04757"
                     /db_xref="CDD:398431"
     Region          275..328
                     /region_name="mRING_PEX12"
                     /note="Modified RING finger found in peroxin-12 (PEX12)
                     and similar proteins; cd16451"
                     /db_xref="CDD:438115"
     Site            order(277,280,298,301)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:438115"
     CDS             1..330
                     /gene="Pex12"
                     /coded_by="XM_034334484.2:480..1472"
                     /db_xref="GeneID:117608838"
ORIGIN      
        1 maekgayltg taftkpsife iiaqesfast vepafkkils fivsfnhgry ehvlkwadec
       61 ylifnvilqr yylkkhsasf seafyslkri aienssnqre lskqqkgisl illvlfpylk
      121 nkffqlsqry klekidgylp qtkweklyri fivkgytvtf mlyefmvlyn yvlyisgksv
      181 ftspllrlls isltytepkm visvsdllrk iknnsfgisd gldifqrvvt tslefgaffl
      241 qflswwnqeh yftnlmslpv ppppvipeia kkykgmcpic cktprvhtvl svsgyafcyq
      301 cilsvirksg kcpvtnypak eddlirlyld
//
LOCUS       XP_034190377             330 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 12 [Osmia lignaria lignaria].
ACCESSION   XP_034190377
VERSION     XP_034190377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="peroxisomal biogenesis factor 12"
                     /calculated_mol_wt=38165
     Region          24..249
                     /region_name="Pex2_Pex12"
                     /note="Pex2 / Pex12 amino terminal region; pfam04757"
                     /db_xref="CDD:398431"
     Region          275..328
                     /region_name="mRING_PEX12"
                     /note="Modified RING finger found in peroxin-12 (PEX12)
                     and similar proteins; cd16451"
                     /db_xref="CDD:438115"
     Site            order(277,280,298,301)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:438115"
     CDS             1..330
                     /gene="Pex12"
                     /coded_by="XM_034334486.2:476..1468"
                     /db_xref="GeneID:117608838"
ORIGIN      
        1 maekgayltg taftkpsife iiaqesfast vepafkkils fivsfnhgry ehvlkwadec
       61 ylifnvilqr yylkkhsasf seafyslkri aienssnqre lskqqkgisl illvlfpylk
      121 nkffqlsqry klekidgylp qtkweklyri fivkgytvtf mlyefmvlyn yvlyisgksv
      181 ftspllrlls isltytepkm visvsdllrk iknnsfgisd gldifqrvvt tslefgaffl
      241 qflswwnqeh yftnlmslpv ppppvipeia kkykgmcpic cktprvhtvl svsgyafcyq
      301 cilsvirksg kcpvtnypak eddlirlyld
//
LOCUS       XP_034190378             330 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 12 [Osmia lignaria lignaria].
ACCESSION   XP_034190378
VERSION     XP_034190378.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334487.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="peroxisomal biogenesis factor 12"
                     /calculated_mol_wt=38165
     Region          24..249
                     /region_name="Pex2_Pex12"
                     /note="Pex2 / Pex12 amino terminal region; pfam04757"
                     /db_xref="CDD:398431"
     Region          275..328
                     /region_name="mRING_PEX12"
                     /note="Modified RING finger found in peroxin-12 (PEX12)
                     and similar proteins; cd16451"
                     /db_xref="CDD:438115"
     Site            order(277,280,298,301)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:438115"
     CDS             1..330
                     /gene="Pex12"
                     /coded_by="XM_034334487.2:163..1155"
                     /db_xref="GeneID:117608838"
ORIGIN      
        1 maekgayltg taftkpsife iiaqesfast vepafkkils fivsfnhgry ehvlkwadec
       61 ylifnvilqr yylkkhsasf seafyslkri aienssnqre lskqqkgisl illvlfpylk
      121 nkffqlsqry klekidgylp qtkweklyri fivkgytvtf mlyefmvlyn yvlyisgksv
      181 ftspllrlls isltytepkm visvsdllrk iknnsfgisd gldifqrvvt tslefgaffl
      241 qflswwnqeh yftnlmslpv ppppvipeia kkykgmcpic cktprvhtvl svsgyafcyq
      301 cilsvirksg kcpvtnypak eddlirlyld
//
LOCUS       XP_034190374             330 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 12 [Osmia lignaria lignaria].
ACCESSION   XP_034190374
VERSION     XP_034190374.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334483.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="peroxisomal biogenesis factor 12"
                     /calculated_mol_wt=38165
     Region          24..249
                     /region_name="Pex2_Pex12"
                     /note="Pex2 / Pex12 amino terminal region; pfam04757"
                     /db_xref="CDD:398431"
     Region          275..328
                     /region_name="mRING_PEX12"
                     /note="Modified RING finger found in peroxin-12 (PEX12)
                     and similar proteins; cd16451"
                     /db_xref="CDD:438115"
     Site            order(277,280,298,301)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:438115"
     CDS             1..330
                     /gene="Pex12"
                     /coded_by="XM_034334483.2:165..1157"
                     /db_xref="GeneID:117608838"
ORIGIN      
        1 maekgayltg taftkpsife iiaqesfast vepafkkils fivsfnhgry ehvlkwadec
       61 ylifnvilqr yylkkhsasf seafyslkri aienssnqre lskqqkgisl illvlfpylk
      121 nkffqlsqry klekidgylp qtkweklyri fivkgytvtf mlyefmvlyn yvlyisgksv
      181 ftspllrlls isltytepkm visvsdllrk iknnsfgisd gldifqrvvt tslefgaffl
      241 qflswwnqeh yftnlmslpv ppppvipeia kkykgmcpic cktprvhtvl svsgyafcyq
      301 cilsvirksg kcpvtnypak eddlirlyld
//
LOCUS       XP_034190379             330 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal biogenesis factor 12 [Osmia lignaria lignaria].
ACCESSION   XP_034190379
VERSION     XP_034190379.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334488.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="peroxisomal biogenesis factor 12"
                     /calculated_mol_wt=38165
     Region          24..249
                     /region_name="Pex2_Pex12"
                     /note="Pex2 / Pex12 amino terminal region; pfam04757"
                     /db_xref="CDD:398431"
     Region          275..328
                     /region_name="mRING_PEX12"
                     /note="Modified RING finger found in peroxin-12 (PEX12)
                     and similar proteins; cd16451"
                     /db_xref="CDD:438115"
     Site            order(277,280,298,301)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:438115"
     CDS             1..330
                     /gene="Pex12"
                     /coded_by="XM_034334488.2:43..1035"
                     /db_xref="GeneID:117608838"
ORIGIN      
        1 maekgayltg taftkpsife iiaqesfast vepafkkils fivsfnhgry ehvlkwadec
       61 ylifnvilqr yylkkhsasf seafyslkri aienssnqre lskqqkgisl illvlfpylk
      121 nkffqlsqry klekidgylp qtkweklyri fivkgytvtf mlyefmvlyn yvlyisgksv
      181 ftspllrlls isltytepkm visvsdllrk iknnsfgisd gldifqrvvt tslefgaffl
      241 qflswwnqeh yftnlmslpv ppppvipeia kkykgmcpic cktprvhtvl svsgyafcyq
      301 cilsvirksg kcpvtnypak eddlirlyld
//
LOCUS       XP_034190516             215 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab11 [Osmia lignaria lignaria].
ACCESSION   XP_034190516
VERSION     XP_034190516.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334625.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="RAS oncogene family member Rab11"
                     /calculated_mol_wt=24206
     Region          9..173
                     /region_name="Rab11_like"
                     /note="Rab GTPase family 11 (Rab11)-like includes Rab11a,
                     Rab11b, and Rab25; cd01868"
                     /db_xref="CDD:206660"
     Site            9..13
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206660"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206660"
     Site            order(20..26,37,40,43,69,124..125,127,154..156)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206660"
     Site            order(26..37,39..42)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206660"
     Site            order(36,41..49)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206660"
     Site            order(41,45..52,59,61)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206660"
     Site            43
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206660"
     Site            order(44,46..48,63,65,72..73,76,80,82..85,172)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206660"
     Site            order(44..45,47,65..66,73,75,77..79)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206660"
     Site            44..48
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206660"
     Site            61..65
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206660"
     Site            66..69
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206660"
     Site            order(69,71..81)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206660"
     Site            72..77
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206660"
     Site            80..84
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206660"
     Site            89..94
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206660"
     Site            117..121
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206660"
     Site            124..127
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206660"
     Site            154..156
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206660"
     Site            170..173
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206660"
     CDS             1..215
                     /gene="Rab11"
                     /coded_by="XM_034334625.2:288..935"
                     /db_xref="GeneID:117608894"
ORIGIN      
        1 mgtrddeydy lfkvvligds gvgksnllsr ftrnefnles kstigvefat rsiqvdgkti
       61 kaqiwdtagq eryraitsay yrgavgallv ydiakhltye nverwlrelr dhadqnivim
      121 lvgnksdlrh lravptdeak afaernglsf ietsaldstn vetafqnilt eiyrivsqkq
      181 irdppegdti rpqnvepidv sptmnsegmr kpccq
//
LOCUS       XP_034190517             215 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab11 [Osmia lignaria lignaria].
ACCESSION   XP_034190517
VERSION     XP_034190517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334626.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="RAS oncogene family member Rab11"
                     /calculated_mol_wt=24206
     Region          9..173
                     /region_name="Rab11_like"
                     /note="Rab GTPase family 11 (Rab11)-like includes Rab11a,
                     Rab11b, and Rab25; cd01868"
                     /db_xref="CDD:206660"
     Site            9..13
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206660"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206660"
     Site            order(20..26,37,40,43,69,124..125,127,154..156)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206660"
     Site            order(26..37,39..42)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206660"
     Site            order(36,41..49)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206660"
     Site            order(41,45..52,59,61)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206660"
     Site            43
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206660"
     Site            order(44,46..48,63,65,72..73,76,80,82..85,172)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206660"
     Site            order(44..45,47,65..66,73,75,77..79)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206660"
     Site            44..48
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206660"
     Site            61..65
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206660"
     Site            66..69
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206660"
     Site            order(69,71..81)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206660"
     Site            72..77
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206660"
     Site            80..84
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206660"
     Site            89..94
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206660"
     Site            117..121
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206660"
     Site            124..127
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206660"
     Site            154..156
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206660"
     Site            170..173
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206660"
     CDS             1..215
                     /gene="Rab11"
                     /coded_by="XM_034334626.2:289..936"
                     /db_xref="GeneID:117608894"
ORIGIN      
        1 mgtrddeydy lfkvvligds gvgksnllsr ftrnefnles kstigvefat rsiqvdgkti
       61 kaqiwdtagq eryraitsay yrgavgallv ydiakhltye nverwlrelr dhadqnivim
      121 lvgnksdlrh lravptdeak afaernglsf ietsaldstn vetafqnilt eiyrivsqkq
      181 irdppegdti rpqnvepidv sptmnsegmr kpccq
//
LOCUS       XP_076545921             215 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab11 [Osmia lignaria lignaria].
ACCESSION   XP_076545921
VERSION     XP_076545921.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689806.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="RAS oncogene family member Rab11"
                     /calculated_mol_wt=24206
     Region          9..173
                     /region_name="Rab11_like"
                     /note="Rab GTPase family 11 (Rab11)-like includes Rab11a,
                     Rab11b, and Rab25; cd01868"
                     /db_xref="CDD:206660"
     Site            9..13
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206660"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206660"
     Site            order(20..26,37,40,43,69,124..125,127,154..156)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206660"
     Site            order(26..37,39..42)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206660"
     Site            order(36,41..49)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206660"
     Site            order(41,45..52,59,61)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206660"
     Site            43
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206660"
     Site            order(44,46..48,63,65,72..73,76,80,82..85,172)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206660"
     Site            order(44..45,47,65..66,73,75,77..79)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206660"
     Site            44..48
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206660"
     Site            61..65
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206660"
     Site            66..69
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206660"
     Site            order(69,71..81)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206660"
     Site            72..77
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206660"
     Site            80..84
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206660"
     Site            89..94
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206660"
     Site            117..121
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206660"
     Site            124..127
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206660"
     Site            154..156
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206660"
     Site            170..173
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206660"
     CDS             1..215
                     /gene="Rab11"
                     /coded_by="XM_076689806.1:289..936"
                     /db_xref="GeneID:117608894"
ORIGIN      
        1 mgtrddeydy lfkvvligds gvgksnllsr ftrnefnles kstigvefat rsiqvdgkti
       61 kaqiwdtagq eryraitsay yrgavgallv ydiakhltye nverwlrelr dhadqnivim
      121 lvgnksdlrh lravptdeak afaernglsf ietsaldstn vetafqnilt eiyrivsqkq
      181 irdppegdti rpqnvepidv sptmnsegmr kpccq
//
LOCUS       XP_034190518             201 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger AN1-type doctor no [Osmia lignaria lignaria].
ACCESSION   XP_034190518
VERSION     XP_034190518.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334627.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="zinc finger AN1-type doctor no"
                     /calculated_mol_wt=21443
     Region          11..33
                     /region_name="zf-A20"
                     /note="A20-like zinc finger; pfam01754"
                     /db_xref="CDD:460313"
     Region          142..179
                     /region_name="ZnF_AN1"
                     /note="AN1-like Zinc finger; smart00154"
                     /db_xref="CDD:197545"
     CDS             1..201
                     /gene="drn"
                     /coded_by="XM_034334627.2:450..1055"
                     /db_xref="GeneID:117608895"
ORIGIN      
        1 meresnpmqa lcrsgcgfyg spatdglcsl cykenlkkkq qppvsastvp psqtvsgnag
       61 tlqggfgspa atgttaqpti ptipqpttdl pnpkeinred qesevsissg avaegsvssg
      121 daddcfdgke tdketkkkkn rcavcrkkvg ltgfecrcgg lfcsvhrysd khdckfdyre
      181 mgaqeirrnn pvvvgekvqk i
//
LOCUS       XP_034190519             201 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger AN1-type doctor no [Osmia lignaria lignaria].
ACCESSION   XP_034190519
VERSION     XP_034190519.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334628.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="zinc finger AN1-type doctor no"
                     /calculated_mol_wt=21443
     Region          11..33
                     /region_name="zf-A20"
                     /note="A20-like zinc finger; pfam01754"
                     /db_xref="CDD:460313"
     Region          142..179
                     /region_name="ZnF_AN1"
                     /note="AN1-like Zinc finger; smart00154"
                     /db_xref="CDD:197545"
     CDS             1..201
                     /gene="drn"
                     /coded_by="XM_034334628.2:399..1004"
                     /db_xref="GeneID:117608895"
ORIGIN      
        1 meresnpmqa lcrsgcgfyg spatdglcsl cykenlkkkq qppvsastvp psqtvsgnag
       61 tlqggfgspa atgttaqpti ptipqpttdl pnpkeinred qesevsissg avaegsvssg
      121 daddcfdgke tdketkkkkn rcavcrkkvg ltgfecrcgg lfcsvhrysd khdckfdyre
      181 mgaqeirrnn pvvvgekvqk i
//
LOCUS       XP_034190520             201 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger AN1-type doctor no [Osmia lignaria lignaria].
ACCESSION   XP_034190520
VERSION     XP_034190520.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334629.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="zinc finger AN1-type doctor no"
                     /calculated_mol_wt=21443
     Region          11..33
                     /region_name="zf-A20"
                     /note="A20-like zinc finger; pfam01754"
                     /db_xref="CDD:460313"
     Region          142..179
                     /region_name="ZnF_AN1"
                     /note="AN1-like Zinc finger; smart00154"
                     /db_xref="CDD:197545"
     CDS             1..201
                     /gene="drn"
                     /coded_by="XM_034334629.2:533..1138"
                     /db_xref="GeneID:117608895"
ORIGIN      
        1 meresnpmqa lcrsgcgfyg spatdglcsl cykenlkkkq qppvsastvp psqtvsgnag
       61 tlqggfgspa atgttaqpti ptipqpttdl pnpkeinred qesevsissg avaegsvssg
      121 daddcfdgke tdketkkkkn rcavcrkkvg ltgfecrcgg lfcsvhrysd khdckfdyre
      181 mgaqeirrnn pvvvgekvqk i
//
LOCUS       XP_034190380             200 aa            linear   INV 03-SEP-2025
DEFINITION  negative cofactor 2beta isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190380
VERSION     XP_034190380.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..200
                     /product="negative cofactor 2beta isoform X1"
                     /calculated_mol_wt=22889
     Region          44..170
                     /region_name="HFD_Dr1"
                     /note="histone-fold domain found in protein Dr1 and
                     similar proteins; cd22905"
                     /db_xref="CDD:467030"
     Site            order(45,47..50,53,57..58,60..62,66..68,71,74..75,78..79,
                     81..84,87..88,91,101..104,107,119,122,126,130)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467030"
     Site            order(48,50..52,99..101,131,137..138)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467030"
     Site            order(151,154..155,157..158,161..162,165..166,169)
                     /site_type="other"
                     /note="TBP binding site [polypeptide binding]"
                     /db_xref="CDD:467030"
     CDS             1..200
                     /gene="NC2beta"
                     /coded_by="XM_034334489.2:48..650"
                     /db_xref="GeneID:117608840"
ORIGIN      
        1 mhendqqtev mddnnkrtde nyrkseenkk keemasatis pteddeltlp rasinkmike
       61 ilphvrvane srelilncct efihllssea neicnqqqkk tinaehvlqa leklgfgdys
      121 aeaeavlrdc kavaakrrrq strlenlgip eeellrqqqe lfakareeqa vaeqqqwqql
      181 qavaqmasmq qadseqedys
//
LOCUS       XP_034190381             190 aa            linear   INV 03-SEP-2025
DEFINITION  negative cofactor 2beta isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190381
VERSION     XP_034190381.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334490.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..190
                     /product="negative cofactor 2beta isoform X2"
                     /calculated_mol_wt=21677
     Region          34..160
                     /region_name="HFD_Dr1"
                     /note="histone-fold domain found in protein Dr1 and
                     similar proteins; cd22905"
                     /db_xref="CDD:467030"
     Site            order(35,37..40,43,47..48,50..52,56..58,61,64..65,68..69,
                     71..74,77..78,81,91..94,97,109,112,116,120)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467030"
     Site            order(38,40..42,89..91,121,127..128)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467030"
     Site            order(141,144..145,147..148,151..152,155..156,159)
                     /site_type="other"
                     /note="TBP binding site [polypeptide binding]"
                     /db_xref="CDD:467030"
     CDS             1..190
                     /gene="NC2beta"
                     /coded_by="XM_034334490.2:79..651"
                     /db_xref="GeneID:117608840"
ORIGIN      
        1 mddnnkrtde nyrkseenkk keemasatis pteddeltlp rasinkmike ilphvrvane
       61 srelilncct efihllssea neicnqqqkk tinaehvlqa leklgfgdys aeaeavlrdc
      121 kavaakrrrq strlenlgip eeellrqqqe lfakareeqa vaeqqqwqql qavaqmasmq
      181 qadseqedys
//
LOCUS       XP_034190362             143 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase, subunit C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190362
VERSION     XP_034190362.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334471.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="ATP synthase, subunit C isoform X1"
                     /calculated_mol_wt=14865
     Region          69..143
                     /region_name="ATP9"
                     /note="ATP synthase F0 subunit 9; Provisional; MTH00222"
                     /db_xref="CDD:164765"
     CDS             1..143
                     /gene="ATPsynC"
                     /coded_by="XM_034334471.2:202..633"
                     /db_xref="GeneID:117608830"
ORIGIN      
        1 mfactrfiap iarstlvsgt ksyirplssa vvshsqsvqq nqiqspvsvs plmqssivrs
       61 fqtstisrdi dsaakfigag aatvgvagsg agigsvfgsl iigyarnpsl kqqlfsyail
      121 gfalseamgl fclmmaflll faf
//
LOCUS       XP_034190363             140 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase, subunit C isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190363
VERSION     XP_034190363.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334472.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="ATP synthase, subunit C isoform X2"
                     /calculated_mol_wt=14580
     Region          66..140
                     /region_name="ATP9"
                     /note="ATP synthase F0 subunit 9; Provisional; MTH00222"
                     /db_xref="CDD:164765"
     CDS             1..140
                     /gene="ATPsynC"
                     /coded_by="XM_034334472.2:202..624"
                     /db_xref="GeneID:117608830"
ORIGIN      
        1 mfactrfiap iarstlvsgt ksyirplssa vvshsqsvqq nqiqspsplm qssivrsfqt
       61 stisrdidsa akfigagaat vgvagsgagi gsvfgsliig yarnpslkqq lfsyailgfa
      121 lseamglfcl mmaflllfaf
//
LOCUS       XP_034190522             142 aa            linear   INV 03-SEP-2025
DEFINITION  small ribonucleoprotein particle protein SmD3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190522
VERSION     XP_034190522.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334631.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..142
                     /product="small ribonucleoprotein particle protein SmD3"
                     /calculated_mol_wt=15333
     Region          6..75
                     /region_name="Sm_D3"
                     /note="Sm protein D3; cd01721"
                     /db_xref="CDD:212468"
     Site            order(18..24,26..38,40..44)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212468"
     Site            order(21..24,26,28,34..35,40,42,60..70)
                     /site_type="other"
                     /note="putative hexamer interface [polypeptide binding]"
                     /db_xref="CDD:212468"
     Site            order(26,28,36,39,42,50,58,60..73)
                     /site_type="other"
                     /note="heptamer interface [polypeptide binding]"
                     /db_xref="CDD:212468"
     Site            order(38,40,51,64..66)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212468"
     Site            60..71
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212468"
     CDS             1..142
                     /gene="SmD3"
                     /coded_by="XM_034334631.2:555..983"
                     /db_xref="GeneID:117608896"
ORIGIN      
        1 msigvpikvl heaeghivtc etntgevyrg klieaednmn cqmqnitvty rdgrevelen
       61 vyirgskirf lilpdmlkna pmfkrpggkg sgtagrgksa ilraqargrg rgqnqrgrgt
      121 gsapwlnqqn qpggsqtgrg rg
//
LOCUS       XP_034185587            1234 aa            linear   INV 03-SEP-2025
DEFINITION  myTH4 and RhoGAP_KIAA1688 domain-containing protein RhoGAP93B
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185587
VERSION     XP_034185587.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329696.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185587.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1234
                     /product="myTH4 and RhoGAP_KIAA1688 domain-containing
                     protein RhoGAP93B isoform X1"
                     /calculated_mol_wt=140523
     Region          48..74
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          856..1009
                     /region_name="MyTH4"
                     /note="MyTH4 domain; cl02480"
                     /db_xref="CDD:470587"
     Region          1032..1223
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1076,1113,1117,1187,1190..1191,1215)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1076
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1234
                     /gene="RhoGAP93B"
                     /coded_by="XM_034329696.2:130..3834"
                     /db_xref="GeneID:117606792"
ORIGIN      
        1 masdgrmewv eiieprtkeh myanlttgec vwdpppgvpv kktdnnqwwe lfdqntsrfy
       61 yynatsqktv whrpsdcdii plaklqtlkq ntepasssgi dsqksgeprr kesvstqtqt
      121 psisrltrfn hqensnlsss lqsgnvnktr tssvgvdlqt sppspspssr rhhhhhhhhn
      181 nrhhrhhevp tarrqhnhsq dsgrssdssv shsrtslest gyrlldsphr hhhrpggqvp
      241 gqaqssprqh sgtlearghk sgtlesvknq ksvplgeppp lglslststp ifkkksfvdt
      301 qresrtgnle leknkngres srgsygpeps tspyrdslme srifrqempp yrppevqlsh
      361 syksqgekyg slvessnryh rdreremhhr ervnsldktn tsafypssmh prnmnkqdnt
      421 gsigrrhhgc svslseanvr dmygamlaer nepskslvrg asvlsnaskq rsievverer
      481 ererererer ererekerer ererererer ererererer ererdrekey rrntacnnsl
      541 dcvsqplars ysfvqqqkqq qklqqlhqqq qqqsrrrdrd ddsmheryli sqsheqprqr
      601 lssntsssns snsssnsavg eetegltegd dskkkrsngn pspppspfyg nllsdadhll
      661 plqhyilqqa klsgcykfgd pllveegdds ldeeggrgeg iggradddsd dqfaddeaas
      721 nqgdsssqey ledhyadlgn ydtaglatyy ntadtltrpq vrppsppiia sqaeiresvt
      781 tarqpplpap ttitsvpsvg tgvdntdide arrddsaggg diekyaqdnl nlncgrpkgl
      841 rllfrkkfsv rdilswskdp ipqpmlvvvd gekllkreac nlfrlvqvym gdrkanvemt
      901 ldsvamdivn tafskpplrd elyvqicrqt tenprkeslr rgwelmavcl afvppsatfe
      961 pylegymnrh rdsnfqfsev akwpihvqvs hyatvacrrl qrigahgkrq prkatiediv
     1021 qariqifras mfgatlsevm alqrdrfpnr elpwiqttlt rqvlarggil tegifrvsad
     1081 adevsilksy ldrfeddnsp asqdahapas llklwvrely eplipdsfyt ecvsmrhddv
     1141 easavnvaal vdrlpdlnrr vlchlirflq ifarpevvar tkmdannlam vmapnilrct
     1201 sqdprvilen arkemafvrt liesletawv ddlh
//
LOCUS       XP_034185588            1228 aa            linear   INV 03-SEP-2025
DEFINITION  myTH4 and RhoGAP_KIAA1688 domain-containing protein RhoGAP93B
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185588
VERSION     XP_034185588.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329697.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185588.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1228
                     /product="myTH4 and RhoGAP_KIAA1688 domain-containing
                     protein RhoGAP93B isoform X2"
                     /calculated_mol_wt=139906
     Region          42..68
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          850..1003
                     /region_name="MyTH4"
                     /note="MyTH4 domain; cl02480"
                     /db_xref="CDD:470587"
     Region          1026..1217
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1070,1107,1111,1181,1184..1185,1209)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1070
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1228
                     /gene="RhoGAP93B"
                     /coded_by="XM_034329697.2:615..4301"
                     /db_xref="GeneID:117606792"
ORIGIN      
        1 mewveiiepr tkehmyanlt tgecvwdppp gvpvkktdnn qwwelfdqnt srfyyynats
       61 qktvwhrpsd cdiiplaklq tlkqntepas ssgidsqksg eprrkesvst qtqtpsisrl
      121 trfnhqensn lssslqsgnv nktrtssvgv dlqtsppsps pssrrhhhhh hhhnnrhhrh
      181 hevptarrqh nhsqdsgrss dssvshsrts lestgyrlld sphrhhhrpg gqvpgqaqss
      241 prqhsgtlea rghksgtles vknqksvplg eppplglsls tstpifkkks fvdtqresrt
      301 gnleleknkn gressrgsyg pepstspyrd slmesrifrq emppyrppev qlshsyksqg
      361 ekygslvess nryhrdrere mhhrervnsl dktntsafyp ssmhprnmnk qdntgsigrr
      421 hhgcsvslse anvrdmygam laernepsks lvrgasvlsn askqrsievv ererererer
      481 ererererek ererererer ererererer ererererdr ekeyrrntac nnsldcvsqp
      541 larsysfvqq qkqqqklqql hqqqqqqsrr rdrdddsmhe rylisqsheq prqrlssnts
      601 ssnssnsssn savgeetegl tegddskkkr sngnpsppps pfygnllsda dhllplqhyi
      661 lqqaklsgcy kfgdpllvee gddsldeegg rgegiggrad ddsddqfadd eaasnqgdss
      721 sqeyledhya dlgnydtagl atyyntadtl trpqvrppsp piiasqaeir esvttarqpp
      781 lpapttitsv psvgtgvdnt didearrdds agggdiekya qdnlnlncgr pkglrllfrk
      841 kfsvrdilsw skdpipqpml vvvdgekllk reacnlfrlv qvymgdrkan vemtldsvam
      901 divntafskp plrdelyvqi crqttenprk eslrrgwelm avclafvpps atfepylegy
      961 mnrhrdsnfq fsevakwpih vqvshyatva crrlqrigah gkrqprkati edivqariqi
     1021 frasmfgatl sevmalqrdr fpnrelpwiq ttltrqvlar ggiltegifr vsadadevsi
     1081 lksyldrfed dnspasqdah apasllklwv relyeplipd sfytecvsmr hddveasavn
     1141 vaalvdrlpd lnrrvlchli rflqifarpe vvartkmdan nlamvmapni lrctsqdprv
     1201 ilenarkema fvrtliesle tawvddlh
//
LOCUS       XP_034185589            1228 aa            linear   INV 03-SEP-2025
DEFINITION  myTH4 and RhoGAP_KIAA1688 domain-containing protein RhoGAP93B
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185589
VERSION     XP_034185589.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329698.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185589.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1228
                     /product="myTH4 and RhoGAP_KIAA1688 domain-containing
                     protein RhoGAP93B isoform X2"
                     /calculated_mol_wt=139906
     Region          42..68
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          850..1003
                     /region_name="MyTH4"
                     /note="MyTH4 domain; cl02480"
                     /db_xref="CDD:470587"
     Region          1026..1217
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1070,1107,1111,1181,1184..1185,1209)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1070
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1228
                     /gene="RhoGAP93B"
                     /coded_by="XM_034329698.2:152..3838"
                     /db_xref="GeneID:117606792"
ORIGIN      
        1 mewveiiepr tkehmyanlt tgecvwdppp gvpvkktdnn qwwelfdqnt srfyyynats
       61 qktvwhrpsd cdiiplaklq tlkqntepas ssgidsqksg eprrkesvst qtqtpsisrl
      121 trfnhqensn lssslqsgnv nktrtssvgv dlqtsppsps pssrrhhhhh hhhnnrhhrh
      181 hevptarrqh nhsqdsgrss dssvshsrts lestgyrlld sphrhhhrpg gqvpgqaqss
      241 prqhsgtlea rghksgtles vknqksvplg eppplglsls tstpifkkks fvdtqresrt
      301 gnleleknkn gressrgsyg pepstspyrd slmesrifrq emppyrppev qlshsyksqg
      361 ekygslvess nryhrdrere mhhrervnsl dktntsafyp ssmhprnmnk qdntgsigrr
      421 hhgcsvslse anvrdmygam laernepsks lvrgasvlsn askqrsievv ererererer
      481 ererererek ererererer ererererer ererererdr ekeyrrntac nnsldcvsqp
      541 larsysfvqq qkqqqklqql hqqqqqqsrr rdrdddsmhe rylisqsheq prqrlssnts
      601 ssnssnsssn savgeetegl tegddskkkr sngnpsppps pfygnllsda dhllplqhyi
      661 lqqaklsgcy kfgdpllvee gddsldeegg rgegiggrad ddsddqfadd eaasnqgdss
      721 sqeyledhya dlgnydtagl atyyntadtl trpqvrppsp piiasqaeir esvttarqpp
      781 lpapttitsv psvgtgvdnt didearrdds agggdiekya qdnlnlncgr pkglrllfrk
      841 kfsvrdilsw skdpipqpml vvvdgekllk reacnlfrlv qvymgdrkan vemtldsvam
      901 divntafskp plrdelyvqi crqttenprk eslrrgwelm avclafvpps atfepylegy
      961 mnrhrdsnfq fsevakwpih vqvshyatva crrlqrigah gkrqprkati edivqariqi
     1021 frasmfgatl sevmalqrdr fpnrelpwiq ttltrqvlar ggiltegifr vsadadevsi
     1081 lksyldrfed dnspasqdah apasllklwv relyeplipd sfytecvsmr hddveasavn
     1141 vaalvdrlpd lnrrvlchli rflqifarpe vvartkmdan nlamvmapni lrctsqdprv
     1201 ilenarkema fvrtliesle tawvddlh
//
LOCUS       XP_034185590            1199 aa            linear   INV 03-SEP-2025
DEFINITION  myTH4 and RhoGAP_KIAA1688 domain-containing protein RhoGAP93B
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185590
VERSION     XP_034185590.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329699.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185590.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1199
                     /product="myTH4 and RhoGAP_KIAA1688 domain-containing
                     protein RhoGAP93B isoform X3"
                     /calculated_mol_wt=136587
     Region          48..74
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          821..974
                     /region_name="MyTH4"
                     /note="MyTH4 domain; cl02480"
                     /db_xref="CDD:470587"
     Region          997..1188
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1041,1078,1082,1152,1155..1156,1180)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            1041
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..1199
                     /gene="RhoGAP93B"
                     /coded_by="XM_034329699.2:130..3729"
                     /db_xref="GeneID:117606792"
ORIGIN      
        1 masdgrmewv eiieprtkeh myanlttgec vwdpppgvpv kktdnnqwwe lfdqntsrfy
       61 yynatsqktv whrpsdcdii plaklqtlkq ntepasssgi dsqksgeprr kesvstqtqt
      121 psisrltrfn hqensnlsss lqsgnvnktr tssvgvdlqt sppspspssr rhhhhhhhhn
      181 nrhhrhhevp tarrqhnhsq dsgrssdssv shsrtslest gyrlldsphr hhhrpggqvp
      241 gqaqssprqh sgtlearghk sgtlesvknq ksvplgeppp lgressrgsy gpepstspyr
      301 dslmesrifr qemppyrppe vqlshsyksq gekygslves snryhrdrer emhhrervns
      361 ldktntsafy pssmhprnmn kqdntgsigr rhhgcsvsls eanvrdmyga mlaernepsk
      421 slvrgasvls naskqrsiev verererere rerererere kerererere rerererere
      481 rererererd rekeyrrnta cnnsldcvsq plarsysfvq qqkqqqklqq lhqqqqqqsr
      541 rrdrdddsmh erylisqshe qprqrlssnt sssnssnsss nsavgeeteg ltegddskkk
      601 rsngnpsppp spfygnllsd adhllplqhy ilqqaklsgc ykfgdpllve egddsldeeg
      661 grgegiggra dddsddqfad deaasnqgds ssqeyledhy adlgnydtag latyyntadt
      721 ltrpqvrpps ppiiasqaei resvttarqp plpapttits vpsvgtgvdn tdidearrdd
      781 sagggdieky aqdnlnlncg rpkglrllfr kkfsvrdils wskdpipqpm lvvvdgekll
      841 kreacnlfrl vqvymgdrka nvemtldsva mdivntafsk pplrdelyvq icrqttenpr
      901 keslrrgwel mavclafvpp satfepyleg ymnrhrdsnf qfsevakwpi hvqvshyatv
      961 acrrlqriga hgkrqprkat iedivqariq ifrasmfgat lsevmalqrd rfpnrelpwi
     1021 qttltrqvla rggiltegif rvsadadevs ilksyldrfe ddnspasqda hapasllklw
     1081 vrelyeplip dsfytecvsm rhddveasav nvaalvdrlp dlnrrvlchl irflqifarp
     1141 evvartkmda nnlamvmapn ilrctsqdpr vilenarkem afvrtliesl etawvddlh
//
LOCUS       XP_076545922             559 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic triacylglycerol lipase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545922
VERSION     XP_076545922.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689807.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..559
                     /product="pancreatic triacylglycerol lipase isoform X1"
                     /calculated_mol_wt=62327
     Region          90..408
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            207..211
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(209,233,327)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(296,314,324..325)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..559
                     /gene="LOC117606793"
                     /coded_by="XM_076689807.1:521..2200"
                     /db_xref="GeneID:117606793"
ORIGIN      
        1 mmrwkkqgvt egegasilll valapvicaa gildpwqwar sdrievnipw lpfenetrcy
       61 helgclnitr swyhlihrpl nvfplprevi ntrfilytke nptegqvliv ardksikrsn
      121 fspkkktkfi ihgfidtpls nwvkemryel lkhddynviv vdwaggslpl ytqatantrl
      181 vgleiahlik hlqtnygldp ndvhlighsl gahtagyage klggiigrit gldpaepyfq
      241 gmpshlrldy tdaklvdvih tdgksifllg lpgygmsqpc ghldfypnng keqpgctdls
      301 ettpslpltl ikegleeasr vlvacnhvra iklfiesins kcqyvahecs syasflkgec
      361 fscksnnsls cgvmgyhadt spalvrrqam gqdvlsllgs kfffttgked pycrrhyrit
      421 inlarpptae swvqgfmkvt lyaengiird vdltpsgymk lehgstvrmv vaagpsgeig
      481 kikrvelswa ydmdvlqprs lcffwcndrl yvnsvtvdvm elpgrgkrea dftsklcsas
      541 rheyaeiasg staafvdnc
//
LOCUS       XP_076545923             558 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic triacylglycerol lipase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545923
VERSION     XP_076545923.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689808.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..558
                     /product="pancreatic triacylglycerol lipase isoform X2"
                     /calculated_mol_wt=62196
     Region          89..407
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            206..210
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(208,232,326)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(295,313,323..324)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..558
                     /gene="LOC117606793"
                     /coded_by="XM_076689808.1:134..1810"
                     /db_xref="GeneID:117606793"
ORIGIN      
        1 mrwkkqgvte gegasilllv alapvicaag ildpwqwars drievnipwl pfenetrcyh
       61 elgclnitrs wyhlihrpln vfplprevin trfilytken ptegqvliva rdksikrsnf
      121 spkkktkfii hgfidtplsn wvkemryell khddynvivv dwaggslply tqatantrlv
      181 gleiahlikh lqtnygldpn dvhlighslg ahtagyagek lggiigritg ldpaepyfqg
      241 mpshlrldyt daklvdviht dgksifllgl pgygmsqpcg hldfypnngk eqpgctdlse
      301 ttpslpltli kegleeasrv lvacnhvrai klfiesinsk cqyvahecss yasflkgecf
      361 scksnnslsc gvmgyhadts palvrrqamg qdvlsllgsk fffttgkedp ycrrhyriti
      421 nlarpptaes wvqgfmkvtl yaengiirdv dltpsgymkl ehgstvrmvv aagpsgeigk
      481 ikrvelsway dmdvlqprsl cffwcndrly vnsvtvdvme lpgrgkread ftsklcsasr
      541 heyaeiasgs taafvdnc
//
LOCUS       XP_076545924             558 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic triacylglycerol lipase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545924
VERSION     XP_076545924.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689809.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..558
                     /product="pancreatic triacylglycerol lipase isoform X2"
                     /calculated_mol_wt=62196
     Region          89..407
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            206..210
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(208,232,326)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(295,313,323..324)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..558
                     /gene="LOC117606793"
                     /coded_by="XM_076689809.1:170..1846"
                     /db_xref="GeneID:117606793"
ORIGIN      
        1 mrwkkqgvte gegasilllv alapvicaag ildpwqwars drievnipwl pfenetrcyh
       61 elgclnitrs wyhlihrpln vfplprevin trfilytken ptegqvliva rdksikrsnf
      121 spkkktkfii hgfidtplsn wvkemryell khddynvivv dwaggslply tqatantrlv
      181 gleiahlikh lqtnygldpn dvhlighslg ahtagyagek lggiigritg ldpaepyfqg
      241 mpshlrldyt daklvdviht dgksifllgl pgygmsqpcg hldfypnngk eqpgctdlse
      301 ttpslpltli kegleeasrv lvacnhvrai klfiesinsk cqyvahecss yasflkgecf
      361 scksnnslsc gvmgyhadts palvrrqamg qdvlsllgsk fffttgkedp ycrrhyriti
      421 nlarpptaes wvqgfmkvtl yaengiirdv dltpsgymkl ehgstvrmvv aagpsgeigk
      481 ikrvelsway dmdvlqprsl cffwcndrly vnsvtvdvme lpgrgkread ftsklcsasr
      541 heyaeiasgs taafvdnc
//
LOCUS       XP_034185592             556 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic triacylglycerol lipase isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185592
VERSION     XP_034185592.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329701.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185592.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..556
                     /product="pancreatic triacylglycerol lipase isoform X3"
                     /calculated_mol_wt=62060
     Region          90..405
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            207..211
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(209,233,324)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(293,311,321..322)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..556
                     /gene="LOC117606793"
                     /coded_by="XM_034329701.2:516..2186"
                     /db_xref="GeneID:117606793"
ORIGIN      
        1 mmrwkkqgvt egegasilll valapvicaa gildpwqwar sdrievnipw lpfenetrcy
       61 helgclnitr swyhlihrpl nvfplprevi ntrfilytke nptegqvliv ardksikrsn
      121 fspkkktkfi ihgfidtpls nwvkemryel lkhddynviv vdwaggslpl ytqatantrl
      181 vgleiahlik hlqtnygldp ndvhlighsl gahtagyage klggiigrit gldpaepyfq
      241 gmpshlrldy tdaklvdvih tdgksifllg ygmsqpcghl dfypnngkeq pgctdlsett
      301 pslpltlike gleeasrvlv acnhvraikl fiesinskcq yvahecssya sflkgecfsc
      361 ksnnslscgv mgyhadtspa lvrrqamgqd vlsllgskff fttgkedpyc rrhyritinl
      421 arpptaeswv qgfmkvtlya engiirdvdl tpsgymkleh gstvrmvvaa gpsgeigkik
      481 rvelswaydm dvlqprslcf fwcndrlyvn svtvdvmelp grgkreadft sklcsasrhe
      541 yaeiasgsta afvdnc
//
LOCUS       XP_034185595             538 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic lipase-related protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034185595
VERSION     XP_034185595.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329704.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..538
                     /product="pancreatic lipase-related protein 2"
                     /calculated_mol_wt=60116
     Region          63..393
                     /region_name="Lipase"
                     /note="pfam00151"
                     /db_xref="CDD:395099"
     CDS             1..538
                     /gene="LOC117606794"
                     /coded_by="XM_034329704.2:908..2524"
                     /db_xref="GeneID:117606794"
ORIGIN      
        1 mfvnetmlil aytanmmatl pekagsqavd nniekqnitt ttlspeeken qfdlqdqwym
       61 wrcfepygcf yigspwsgen rpvstfparp dtinpryily trdqtepqel kidkfetihq
      121 aplrknsnly livhgfldng dktwvlrtmk elllredsnv vivnwiggag ppytqavant
      181 rlvgamtarl ayqlievgsv npakmhcigh slgahtcgyv gytlrqryey nlgritgldp
      241 aephfsntst mvrldptdat fvtaihtdcn pfisgglgit qpvahidfyp nggrnqpgcn
      301 egvlnsitle rgsffrgikr flgcnhirsy eyfiesvntd cpflavpcss wdkfqegvcf
      361 dcvnqycprf gldaqpgnyh asvylmtgss kpfckghykv tinisktnes ldhggevgtf
      421 virvidendk ksekiplspq skyyepgsth tvvlagevvg kpeaieiswe yqasvfnplt
      481 wrllhtprvy idsltiysle sshgitvcpd atktlianep kilttkncqn adsnlisa
//
LOCUS       XP_076545925             538 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic lipase-related protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_076545925
VERSION     XP_076545925.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689810.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..538
                     /product="pancreatic lipase-related protein 2"
                     /calculated_mol_wt=60116
     Region          63..393
                     /region_name="Lipase"
                     /note="pfam00151"
                     /db_xref="CDD:395099"
     CDS             1..538
                     /gene="LOC117606794"
                     /coded_by="XM_076689810.1:883..2499"
                     /db_xref="GeneID:117606794"
ORIGIN      
        1 mfvnetmlil aytanmmatl pekagsqavd nniekqnitt ttlspeeken qfdlqdqwym
       61 wrcfepygcf yigspwsgen rpvstfparp dtinpryily trdqtepqel kidkfetihq
      121 aplrknsnly livhgfldng dktwvlrtmk elllredsnv vivnwiggag ppytqavant
      181 rlvgamtarl ayqlievgsv npakmhcigh slgahtcgyv gytlrqryey nlgritgldp
      241 aephfsntst mvrldptdat fvtaihtdcn pfisgglgit qpvahidfyp nggrnqpgcn
      301 egvlnsitle rgsffrgikr flgcnhirsy eyfiesvntd cpflavpcss wdkfqegvcf
      361 dcvnqycprf gldaqpgnyh asvylmtgss kpfckghykv tinisktnes ldhggevgtf
      421 virvidendk ksekiplspq skyyepgsth tvvlagevvg kpeaieiswe yqasvfnplt
      481 wrllhtprvy idsltiysle sshgitvcpd atktlianep kilttkncqn adsnlisa
//
LOCUS       XP_076545926             486 aa            linear   INV 03-SEP-2025
DEFINITION  protein RCC2 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545926
VERSION     XP_076545926.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689811.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="protein RCC2 homolog isoform X1"
                     /calculated_mol_wt=52324
     Region          121..464
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     CDS             1..486
                     /gene="LOC117606797"
                     /coded_by="XM_076689811.1:490..1950"
                     /db_xref="GeneID:117606797"
ORIGIN      
        1 msakrksltt kkgrgksrkv eydeedisse hdsdvddaet gsnadgedaa eddltdvsti
       61 pelpppeggw gkpgtllmcg ltnwdmagrk appkgvkvnv grslwtphtf knlngsrvrl
      121 vasgpaashs iivtednrcl afgrndkgql gvgdtkrrde ptevtslkgh tviaascgrn
      181 htlfltsrgi vfaagdnklg qcgvgksdac ivsatkvkys gppivkvgcg aefsmildik
      241 gglhsfgspe ygalghntdg kyfitntkma fhfekvpkri vlyverakdg hvtpldcvei
      301 tdfscghyht vaidsknraf swgfggigrl ghneqrdelv prlikflepp nrgvravhcg
      361 stysiainvh gsalmfgqtk rtgeanmypk piqdlsgwei rcvgcsqtsv vvaaddsvia
      421 wgasptfgel gfgemrksst tpmevkaleg lyitavtcgl shtlmicrde seeekvkink
      481 lqvysv
//
LOCUS       XP_076545927             409 aa            linear   INV 03-SEP-2025
DEFINITION  protein RCC2 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545927
VERSION     XP_076545927.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689812.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="protein RCC2 homolog isoform X2"
                     /calculated_mol_wt=44108
     Region          44..387
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     CDS             1..409
                     /gene="LOC117606797"
                     /coded_by="XM_076689812.1:351..1580"
                     /db_xref="GeneID:117606797"
ORIGIN      
        1 mcgltnwdma grkappkgvk vnvgrslwtp htfknlngsr vrlvasgpaa shsiivtedn
       61 rclafgrndk gqlgvgdtkr rdeptevtsl kghtviaasc grnhtlflts rgivfaagdn
      121 klgqcgvgks dacivsatkv kysgppivkv gcgaefsmil dikgglhsfg speygalghn
      181 tdgkyfitnt kmafhfekvp krivlyvera kdghvtpldc veitdfscgh yhtvaidskn
      241 rafswgfggi grlghneqrd elvprlikfl eppnrgvrav hcgstysiai nvhgsalmfg
      301 qtkrtgeanm ypkpiqdlsg weircvgcsq tsvvvaadds viawgasptf gelgfgemrk
      361 ssttpmevka leglyitavt cglshtlmic rdeseeekvk inklqvysv
//
LOCUS       XP_076545928             409 aa            linear   INV 03-SEP-2025
DEFINITION  protein RCC2 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545928
VERSION     XP_076545928.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689813.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="protein RCC2 homolog isoform X2"
                     /calculated_mol_wt=44108
     Region          44..387
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     CDS             1..409
                     /gene="LOC117606797"
                     /coded_by="XM_076689813.1:306..1535"
                     /db_xref="GeneID:117606797"
ORIGIN      
        1 mcgltnwdma grkappkgvk vnvgrslwtp htfknlngsr vrlvasgpaa shsiivtedn
       61 rclafgrndk gqlgvgdtkr rdeptevtsl kghtviaasc grnhtlflts rgivfaagdn
      121 klgqcgvgks dacivsatkv kysgppivkv gcgaefsmil dikgglhsfg speygalghn
      181 tdgkyfitnt kmafhfekvp krivlyvera kdghvtpldc veitdfscgh yhtvaidskn
      241 rafswgfggi grlghneqrd elvprlikfl eppnrgvrav hcgstysiai nvhgsalmfg
      301 qtkrtgeanm ypkpiqdlsg weircvgcsq tsvvvaadds viawgasptf gelgfgemrk
      361 ssttpmevka leglyitavt cglshtlmic rdeseeekvk inklqvysv
//
LOCUS       XP_076545929             409 aa            linear   INV 03-SEP-2025
DEFINITION  protein RCC2 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545929
VERSION     XP_076545929.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689814.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="protein RCC2 homolog isoform X2"
                     /calculated_mol_wt=44108
     Region          44..387
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     CDS             1..409
                     /gene="LOC117606797"
                     /coded_by="XM_076689814.1:294..1523"
                     /db_xref="GeneID:117606797"
ORIGIN      
        1 mcgltnwdma grkappkgvk vnvgrslwtp htfknlngsr vrlvasgpaa shsiivtedn
       61 rclafgrndk gqlgvgdtkr rdeptevtsl kghtviaasc grnhtlflts rgivfaagdn
      121 klgqcgvgks dacivsatkv kysgppivkv gcgaefsmil dikgglhsfg speygalghn
      181 tdgkyfitnt kmafhfekvp krivlyvera kdghvtpldc veitdfscgh yhtvaidskn
      241 rafswgfggi grlghneqrd elvprlikfl eppnrgvrav hcgstysiai nvhgsalmfg
      301 qtkrtgeanm ypkpiqdlsg weircvgcsq tsvvvaadds viawgasptf gelgfgemrk
      361 ssttpmevka leglyitavt cglshtlmic rdeseeekvk inklqvysv
//
LOCUS       XP_076545930             249 aa            linear   INV 03-SEP-2025
DEFINITION  RNA transcription, translation and transport factor protein-like
            [Osmia lignaria lignaria].
ACCESSION   XP_076545930
VERSION     XP_076545930.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689815.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..249
                     /product="RNA transcription, translation and transport
                     factor protein-like"
                     /calculated_mol_wt=28916
     Region          8..248
                     /region_name="RLL"
                     /note="RNA transcription, translation and transport factor
                     protein; pfam10036"
                     /db_xref="CDD:401864"
     CDS             1..249
                     /gene="LOC143305613"
                     /coded_by="XM_076689815.1:119..868"
                     /db_xref="GeneID:143305613"
ORIGIN      
        1 mfkrrlkalg yvewekvnvn dpqhfrkvvi wledqkirqy siedrkplrn lqsenwpkvf
       61 akycedvkcp isnkpldqle wlvghaiwle aennveeysq nvkemklkmk eeakvpqlrs
      121 knpldnldfy snefktgtya lakllriprh pnhlvtlkac sklvqkrlns eclrnpnski
      181 irgkpfpimd inagfqlnkp tlenaakila llyiqdirnl qtkineaivr vqsitanpkt
      241 dtklgkvgk
//
LOCUS       XP_034185597             223 aa            linear   INV 03-SEP-2025
DEFINITION  ras-related protein Rab-18-B [Osmia lignaria lignaria].
ACCESSION   XP_034185597
VERSION     XP_034185597.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329706.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="ras-related protein Rab-18-B"
                     /calculated_mol_wt=25213
     Region          11..171
                     /region_name="Rab18"
                     /note="Rab GTPase family 18 (Rab18); cd01863"
                     /db_xref="CDD:206656"
     Site            11..12
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206656"
     Site            17..24
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206656"
     Site            order(19..25,35..36,42,68,124..125,127..128,153..155)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206656"
     Site            order(25..36,40..41)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206656"
     Site            order(35,40..48)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206656"
     Site            order(40,44..51,58,60)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206656"
     Site            42
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206656"
     Site            order(43,45..47,62,64,71..72,75,79,81..84,171)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206656"
     Site            order(43..44,46,64..65,72,74,76..78)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206656"
     Site            43..47
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206656"
     Site            60..64
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206656"
     Site            65..68
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206656"
     Site            order(68,70..80)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206656"
     Site            71..76
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206656"
     Site            79..83
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206656"
     Site            88..93
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206656"
     Site            116..121
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206656"
     Site            124..127
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206656"
     Site            153..155
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206656"
     Site            169..171
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206656"
     CDS             1..223
                     /gene="LOC117606795"
                     /coded_by="XM_034329706.2:293..964"
                     /db_xref="GeneID:117606795"
ORIGIN      
        1 mdmdqdvltv lkllmigesn vgkssiilrf tedefyenmq stvgmdyktk qvtidgntvk
       61 laiwdtagqe rfrtltpsyy rdgqgailvy dvtdrvtfvk letwlnelnt ycnktdivkm
      121 vvgnkidlpn revsteeglq farrhqtlyi essaktadgi kccfeelvqk iiqtpglwdr
      181 qpflkaygng smggarhrgq rgiqlandsq phepysncyc slv
//
LOCUS       XP_034185598             209 aa            linear   INV 03-SEP-2025
DEFINITION  AN1-type zinc finger protein 2A [Osmia lignaria lignaria].
ACCESSION   XP_034185598
VERSION     XP_034185598.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329707.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="AN1-type zinc finger protein 2A"
                     /calculated_mol_wt=23555
     Region          10..46
                     /region_name="zf-AN1"
                     /note="AN1-like Zinc finger; pfam01428"
                     /db_xref="CDD:460208"
     Region          100..136
                     /region_name="zf-AN1"
                     /note="AN1-like Zinc finger; pfam01428"
                     /db_xref="CDD:460208"
     Region          171..186
                     /region_name="UIM"
                     /note="Ubiquitin interaction motif; pfam02809"
                     /db_xref="CDD:460703"
     CDS             1..209
                     /gene="LOC117606796"
                     /coded_by="XM_034329707.2:379..1008"
                     /db_xref="GeneID:117606796"
ORIGIN      
        1 mefpnlgehc sekscnrldf lpvkcdacsg ifcaehmsyt dhscpsaykk diqvpvcplc
       61 ntpvptkrgd ppdiavgqhi dnecqselrk prqkifsnrc sskgckikei vqircsdcgk
      121 nfclkhrhpt dhacvgqeea trrkrldald nnvkvnrrng eifktyqgsm sedealaral
      181 qaslqeedta rrrtletvps enrdrcrls
//
LOCUS       XP_034190633            1387 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal RNA Processing 5 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190633
VERSION     XP_034190633.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334742.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190633.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1387
                     /product="ribosomal RNA Processing 5 isoform X1"
                     /calculated_mol_wt=156199
     Region          60..155
                     /region_name="S1_Rrp5_repeat_hs1_sc1"
                     /note="Rrp5 is a trans-acting factor important for
                     biogenesis of both the 40S and 60S eukaryotic ribosomal
                     subunits. Rrp5 has two distinct regions, an N-terminal
                     region containing tandemly repeated S1 RNA-binding domains
                     (12 S1 repeats in Saccharomyces...; cd05693"
                     /db_xref="CDD:240198"
     Site            order(71,79,89,91)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:240198"
     Region          163..233
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(174,182,193,195)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          252..309
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(260,268,278,280)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          329..398
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Region          <413..583
                     /region_name="rpsA"
                     /note="30S ribosomal protein S1; Reviewed; PRK06299"
                     /db_xref="CDD:235775"
     Region          482..930
                     /region_name="rpsA"
                     /note="ribosomal protein S1; TIGR00717"
                     /db_xref="CDD:273232"
     Region          1113..1386
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          1209..1236
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1242..1270
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(1243,1246..1247,1250..1251,1253,1279,1282..1283,
                     1286..1287,1289..1290,1313,1316..1317,1320..1321,1324)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          1277..1307
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1312..1339
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..1387
                     /gene="Rrp5"
                     /coded_by="XM_034334742.2:335..4498"
                     /db_xref="GeneID:117608953"
ORIGIN      
        1 mtlksfprgg kkpldkkssd sqlfknperh nkkkqrsgks dkeeenfvat taerlsylti
       61 seglvvlgcv seateydlii slpggligra qvadisesyt nllqnlinte daqqnefrsl
      121 pelyscgdyv vcyvksiqpq ekwqialsld prlinqnvdv ayledgsrml ctissiedhg
      181 yvvdtgvnnv rafipapknn dkslypgkql lctieeietn entstiklst kqkhaavqnd
      241 teiksldslm pgtkfelsvk kvlsnglyvs fgsshigyin qlylneplsk yiagmeitgt
      301 mlyilptvkf gyfsllikkr rnvdaiqpgh viddattlfr esggialqlt kngtkgfvpl
      361 krtevnydqi iekfvpgtkh kcrvlsyswm damyictmeh slleqkyfsv sdfkpgdvvn
      421 vritsinaes gfvnvqvgki ngqvapehvs degasalkkl ktdkevearv ldvdtarkkv
      481 hftfkksllt snlpvlsdik dakrgsryhg tvvqiskggl lvkffgnvkg wvprtalnte
      541 tfegnwnysi gqtilvlids vnenlgkiil svptkekkkk envftigerv egtiiesstq
      601 gvylrisknd gqgvstgflp aghmspctet atllasksip gdtlsalvfa tvpsliltrt
      661 fltqekyrsf eqlkigdcip csvkdiepng mrvilpvagc tpfgyvsysn vsnfhllqvh
      721 qilfakvisi nrkekqvnlt lslkklfdgl pdlhskimta vdtltlhfnk ltelarnpfy
      781 snrpissvll gqkvtgkvdi itadglvvkl ennllgivsk nhysgnrkvg dtvsgtvmwk
      841 nyvhelvelt mipavmksin eqqdkhreip qdklirgkil mvtnwfvliv lkghgkgsla
      901 ampvkrhvnd vrpdlspyiv hskircyivs npnesdvmpi cmvksafeek lnietkpaan
      961 klkrkkmnse seevpakkvk ksekaapdvk plnkkkkaka ekeenseeid epsknvkqkr
     1021 dkklkkikqd vkppiniitd dydkddekpr ipecgffwdd kpnldlldkq sssdsedete
     1081 kqpkqkkkkl saaenremer qkereirere evlasnqlpk svdqfdrlvl aspdssiiwl
     1141 qymayhlqst eidkaravar kavktisfre enerlnvwna wlnleskfgt peslnsvfqe
     1201 avrsndalkv yshmltvhle agrqfeleki intmigkfkq npqvwidcga allkmglkdk
     1261 srhimqralq slpasehvnl marfatlenk lgdkeraqtl feqilssypk rvdiwscyvd
     1321 slvksndidi arkileravv qtlpprkmrs lfkkfisfee qhgtqedisr vqqmaaeyve
     1381 kqcnqdd
//
LOCUS       XP_034190634            1386 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal RNA Processing 5 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190634
VERSION     XP_034190634.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334743.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190634.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1386
                     /product="ribosomal RNA Processing 5 isoform X2"
                     /calculated_mol_wt=156071
     Region          59..154
                     /region_name="S1_Rrp5_repeat_hs1_sc1"
                     /note="Rrp5 is a trans-acting factor important for
                     biogenesis of both the 40S and 60S eukaryotic ribosomal
                     subunits. Rrp5 has two distinct regions, an N-terminal
                     region containing tandemly repeated S1 RNA-binding domains
                     (12 S1 repeats in Saccharomyces...; cd05693"
                     /db_xref="CDD:240198"
     Site            order(70,78,88,90)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:240198"
     Region          162..232
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(173,181,192,194)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          251..308
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(259,267,277,279)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:238094"
     Region          328..397
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Region          <412..582
                     /region_name="rpsA"
                     /note="30S ribosomal protein S1; Reviewed; PRK06299"
                     /db_xref="CDD:235775"
     Region          481..929
                     /region_name="rpsA"
                     /note="ribosomal protein S1; TIGR00717"
                     /db_xref="CDD:273232"
     Region          1112..1385
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          1208..1235
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1241..1269
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(1242,1245..1246,1249..1250,1252,1278,1281..1282,
                     1285..1286,1288..1289,1312,1315..1316,1319..1320,1323)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          1276..1306
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          1311..1338
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..1386
                     /gene="Rrp5"
                     /coded_by="XM_034334743.2:333..4493"
                     /db_xref="GeneID:117608953"
ORIGIN      
        1 mtlksfprgg kkpldkkssd slfknperhn kkkqrsgksd keeenfvatt aerlsyltis
       61 eglvvlgcvs eateydliis lpggligraq vadisesytn llqnlinted aqqnefrslp
      121 elyscgdyvv cyvksiqpqe kwqialsldp rlinqnvdva yledgsrmlc tissiedhgy
      181 vvdtgvnnvr afipapknnd kslypgkqll ctieeietne ntstiklstk qkhaavqndt
      241 eiksldslmp gtkfelsvkk vlsnglyvsf gsshigyinq lylneplsky iagmeitgtm
      301 lyilptvkfg yfsllikkrr nvdaiqpghv iddattlfre sggialqltk ngtkgfvplk
      361 rtevnydqii ekfvpgtkhk crvlsyswmd amyictmehs lleqkyfsvs dfkpgdvvnv
      421 ritsinaesg fvnvqvgkin gqvapehvsd egasalkklk tdkevearvl dvdtarkkvh
      481 ftfkksllts nlpvlsdikd akrgsryhgt vvqiskggll vkffgnvkgw vprtalntet
      541 fegnwnysig qtilvlidsv nenlgkiils vptkekkkke nvftigerve gtiiesstqg
      601 vylriskndg qgvstgflpa ghmspcteta tllasksipg dtlsalvfat vpsliltrtf
      661 ltqekyrsfe qlkigdcipc svkdiepngm rvilpvagct pfgyvsysnv snfhllqvhq
      721 ilfakvisin rkekqvnltl slkklfdglp dlhskimtav dtltlhfnkl telarnpfys
      781 nrpissvllg qkvtgkvdii tadglvvkle nnllgivskn hysgnrkvgd tvsgtvmwkn
      841 yvhelveltm ipavmksine qqdkhreipq dklirgkilm vtnwfvlivl kghgkgslaa
      901 mpvkrhvndv rpdlspyivh skircyivsn pnesdvmpic mvksafeekl nietkpaank
      961 lkrkkmnses eevpakkvkk sekaapdvkp lnkkkkakae keenseeide psknvkqkrd
     1021 kklkkikqdv kppiniitdd ydkddekpri pecgffwddk pnldlldkqs ssdsedetek
     1081 qpkqkkkkls aaenremerq kereireree vlasnqlpks vdqfdrlvla spdssiiwlq
     1141 ymayhlqste idkaravark avktisfree nerlnvwnaw lnleskfgtp eslnsvfqea
     1201 vrsndalkvy shmltvhlea grqfelekii ntmigkfkqn pqvwidcgaa llkmglkdks
     1261 rhimqralqs lpasehvnlm arfatlenkl gdkeraqtlf eqilssypkr vdiwscyvds
     1321 lvksndidia rkileravvq tlpprkmrsl fkkfisfeeq hgtqedisrv qqmaaeyvek
     1381 qcnqdd
//
LOCUS       XP_034190635             940 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608954 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190635
VERSION     XP_034190635.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334744.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190635.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..940
                     /product="uncharacterized protein LOC117608954 isoform X1"
                     /calculated_mol_wt=102057
     Region          602..931
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(605,610,892,910)
                     /site_type="active"
                     /db_xref="CDD:239139"
     CDS             1..940
                     /gene="LOC117608954"
                     /coded_by="XM_034334744.2:263..3085"
                     /db_xref="GeneID:117608954"
ORIGIN      
        1 msyhrggqag avavsystsp maphspsrrc ssgssgtsss ainsstskln tyrstgsssi
       61 ldrptnfyss sgssgyrsny tseyrrsycp sgsfysstmt gtsirrnyms eysrsrcspa
      121 rsvsssaysn tgsgntgtnv tvnstsssgg ryglstssss ssipitgssr drvqelssss
      181 atrhdtsvsr sdlsryspag yiptirqtss ntagshhhqw rhhstgsslt elfggderei
      241 ersvrdrpds slssssssss aatggtlwsr skrkplssst vltsghvrad saaspgevti
      301 dergsvrdid edddednddd ddddedntdd dqhnnngngs nsnvedacgy ginnndnddd
      361 ddedgdsnln nrhhhqrvgl lhrtdevspa krnllspvpg gaggadfigv kldlltnlat
      421 ntnnatelli nnngqdkvvd dveieaesev irgidgaaas idatasflqg grtagdpeil
      481 edggdhhqqq qqqrnastsy didqtgfggg ttppvlqngv agrpsgtyge dpsvpstsrr
      541 pdghfsgsan tasnltsspt apssahqnly rgvadqfegs ptnksarnrl qahrdercgl
      601 nglrnigntc fmnsviqcls ntrplleyll neqhmadint ttssmkgali kafsqvihel
      661 wevggdhvvn ttalksqiqr faprfmgysq qdaqeflryl leglhedvnr vtvkpqpiht
      721 dipemftdsq kaveswkryl rsedsmvvdv fvgqlrsslr ctscdhvsvt ldpfwdlslp
      781 iparngtvkl sqclehftre evldgdekpt cskcqmrrkc tksfsiqkfp kilvihlkrf
      841 spmeryrgkl nvmvdfpltg ldlsafaapr vpgctynlyg vanhsgttys ghytayckhp
      901 ysgewheynd srvsvvpars vvsseayvlf yeqqphsshl
//
LOCUS       XP_034190636             940 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608954 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190636
VERSION     XP_034190636.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190636.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..940
                     /product="uncharacterized protein LOC117608954 isoform X1"
                     /calculated_mol_wt=102057
     Region          602..931
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(605,610,892,910)
                     /site_type="active"
                     /db_xref="CDD:239139"
     CDS             1..940
                     /gene="LOC117608954"
                     /coded_by="XM_034334745.2:470..3292"
                     /db_xref="GeneID:117608954"
ORIGIN      
        1 msyhrggqag avavsystsp maphspsrrc ssgssgtsss ainsstskln tyrstgsssi
       61 ldrptnfyss sgssgyrsny tseyrrsycp sgsfysstmt gtsirrnyms eysrsrcspa
      121 rsvsssaysn tgsgntgtnv tvnstsssgg ryglstssss ssipitgssr drvqelssss
      181 atrhdtsvsr sdlsryspag yiptirqtss ntagshhhqw rhhstgsslt elfggderei
      241 ersvrdrpds slssssssss aatggtlwsr skrkplssst vltsghvrad saaspgevti
      301 dergsvrdid edddednddd ddddedntdd dqhnnngngs nsnvedacgy ginnndnddd
      361 ddedgdsnln nrhhhqrvgl lhrtdevspa krnllspvpg gaggadfigv kldlltnlat
      421 ntnnatelli nnngqdkvvd dveieaesev irgidgaaas idatasflqg grtagdpeil
      481 edggdhhqqq qqqrnastsy didqtgfggg ttppvlqngv agrpsgtyge dpsvpstsrr
      541 pdghfsgsan tasnltsspt apssahqnly rgvadqfegs ptnksarnrl qahrdercgl
      601 nglrnigntc fmnsviqcls ntrplleyll neqhmadint ttssmkgali kafsqvihel
      661 wevggdhvvn ttalksqiqr faprfmgysq qdaqeflryl leglhedvnr vtvkpqpiht
      721 dipemftdsq kaveswkryl rsedsmvvdv fvgqlrsslr ctscdhvsvt ldpfwdlslp
      781 iparngtvkl sqclehftre evldgdekpt cskcqmrrkc tksfsiqkfp kilvihlkrf
      841 spmeryrgkl nvmvdfpltg ldlsafaapr vpgctynlyg vanhsgttys ghytayckhp
      901 ysgewheynd srvsvvpars vvsseayvlf yeqqphsshl
//
LOCUS       XP_034190637             912 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608954 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190637
VERSION     XP_034190637.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334746.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190637.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..912
                     /product="uncharacterized protein LOC117608954 isoform X2"
                     /calculated_mol_wt=98849
     Region          574..903
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Site            order(577,582,864,882)
                     /site_type="active"
                     /db_xref="CDD:239139"
     CDS             1..912
                     /gene="LOC117608954"
                     /coded_by="XM_034334746.2:261..2999"
                     /db_xref="GeneID:117608954"
ORIGIN      
        1 msyhrggqag avavsystsp maphspsrrc ssgssgtsss ainsstskln tyrstgsssi
       61 ldrptnfyss sgssgyrsny tseyrrsycp sgrsvsssay sntgsgntgt nvtvnstsss
      121 ggryglstss ssssipitgs srdrvqelss ssatrhdtsv srsdlsrysp agyiptirqt
      181 ssntagshhh qwrhhstgss ltelfggder eiersvrdrp dsslssssss ssaatggtlw
      241 srskrkplss stvltsghvr adsaaspgev tidergsvrd idedddednd ddddddednt
      301 dddqhnnngn gsnsnvedac gyginnndnd ddddedgdsn lnnrhhhqrv gllhrtdevs
      361 pakrnllspv pggaggadfi gvkldlltnl atntnnatel linnngqdkv vddveieaes
      421 evirgidgaa asidatasfl qggrtagdpe iledggdhhq qqqqqrnast sydidqtgfg
      481 ggttppvlqn gvagrpsgty gedpsvpsts rrpdghfsgs antasnltss ptapssahqn
      541 lyrgvadqfe gsptnksarn rlqahrderc glnglrnign tcfmnsviqc lsntrplley
      601 llneqhmadi ntttssmkga likafsqvih elwevggdhv vnttalksqi qrfaprfmgy
      661 sqqdaqeflr ylleglhedv nrvtvkpqpi htdipemftd sqkaveswkr ylrsedsmvv
      721 dvfvgqlrss lrctscdhvs vtldpfwdls lpiparngtv klsqclehft reevldgdek
      781 ptcskcqmrr kctksfsiqk fpkilvihlk rfspmeryrg klnvmvdfpl tgldlsafaa
      841 prvpgctynl ygvanhsgtt ysghytayck hpysgewhey ndsrvsvvpa rsvvsseayv
      901 lfyeqqphss hl
//
LOCUS       XP_034185605             929 aa            linear   INV 03-SEP-2025
DEFINITION  SH3 and cysteine-rich domain-containing protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185605
VERSION     XP_034185605.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329714.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..929
                     /product="SH3 and cysteine-rich domain-containing protein
                     isoform X1"
                     /calculated_mol_wt=103354
     Region          113..187
                     /region_name="FCH"
                     /note="Fes/CIP4, and EFC/F-BAR homology domain; pfam00611"
                     /db_xref="CDD:459868"
     Region          546..597
                     /region_name="C1_Stac"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in the SH3 and cysteine-rich domain-containing
                     protein (Stac) family; cd20817"
                     /db_xref="CDD:410367"
     Region          803..853
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(807,809,812,816,834..835,849,851..852)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     CDS             1..929
                     /gene="Stacl"
                     /coded_by="XM_034329714.2:720..3509"
                     /db_xref="GeneID:117606800"
ORIGIN      
        1 myviypadel lriyenqvrd pspvltsdrr lscqvtvipv dgnneqkpkg rkkkwrergq
       61 trkrrtgrne arshevakgn rrdkhtrtmq nsralaedal nplkdlvqpg lneplkqhna
      121 salklvqtvs efaqelgaam enhsamvrrl vdefrgrsgd mrvdsggmrr vwenllrqve
      181 adavahldla avlqqqlsrp tleasfhrkl qsrkvfahre ayeqvvakte eklqrarady
      241 krayatfltn dgggeqelkr ayfeahnayi lqlratnait eryqahclpg llgeiaevye
      301 elcglackcv agiseaaaer ageqakryqa vakeaqviap lndlqvlars laatatpskk
      361 psrrlfvapg ppeqvpmeri nqvpplrdel aptgtstlpl medlrrehdt lsqeisrlqd
      421 aldalvrmqr ksaesnlytk vaelqedism krfelgeaql ylaavqaqke lfgggeagqp
      481 dgvssrkmsn gstgstkhkw lkafkslkts spttppstdk gkqamyhavs tvvamsrksl
      541 esegghqwye ntyrkitpcd acgqvlrghs rqgvrcrrck anahtdcinl vqpamcpsla
      601 pkksggipll rrqktqaqvd etpaaehnvd aiyqvlkqag eirgnstnsp ptpptadhpp
      661 apsstrassh pcssstsaph spqrrrerln lrmkslslds pegtahvrhr prdyyttgqk
      721 estpprhsds gsinrlspcs pgqghavhkh vrgairmssm elpdeneksy sststspcps
      781 phtnnqnhmn ppgkrvlppn nlyvvlynfe arhrdeldlk agykvtvldk sekdwwkgkc
      841 lggrvgyfps ayvmrvesgq ktlqvtrnlq ltdqitllrd qiviqvgeev dgvamvrcaa
      901 dvrsadhtgk egevlyketl cplkylqev
//
LOCUS       XP_034185606             912 aa            linear   INV 03-SEP-2025
DEFINITION  SH3 and cysteine-rich domain-containing protein isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185606
VERSION     XP_034185606.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329715.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..912
                     /product="SH3 and cysteine-rich domain-containing protein
                     isoform X2"
                     /calculated_mol_wt=101536
     Region          113..187
                     /region_name="FCH"
                     /note="Fes/CIP4, and EFC/F-BAR homology domain; pfam00611"
                     /db_xref="CDD:459868"
     Region          529..580
                     /region_name="C1_Stac"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in the SH3 and cysteine-rich domain-containing
                     protein (Stac) family; cd20817"
                     /db_xref="CDD:410367"
     Region          786..836
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(790,792,795,799,817..818,832,834..835)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     CDS             1..912
                     /gene="Stacl"
                     /coded_by="XM_034329715.2:719..3457"
                     /db_xref="GeneID:117606800"
ORIGIN      
        1 myviypadel lriyenqvrd pspvltsdrr lscqvtvipv dgnneqkpkg rkkkwrergq
       61 trkrrtgrne arshevakgn rrdkhtrtmq nsralaedal nplkdlvqpg lneplkqhna
      121 salklvqtvs efaqelgaam enhsamvrrl vdefrgrsgd mrvdsggmrr vwenllrqve
      181 adavahldla avlqqqlsrp tleasfhrkl qsrkvfahre ayeqvvakte eklqrarady
      241 krayatfltn dgggeqelkr ayfeahnayi lqlratnait eryqahclpg llgeiaevye
      301 elcglackcv agiseaaaer ageqakryqa vakeaqviap lndlqvlars laatatpskk
      361 psrrlfvapg ppeqvpmeri nqvpplrdel aptgtstlpl medlrrehdt lsqeisrlqd
      421 aldalvrmqr ksaesnlytk vaelqedism krfelgeaql ylaavqaqke lfgggeagqp
      481 dgvssrkmsn gstgstkhkw lkafkslkts spttppstdk kslesegghq wyentyrkit
      541 pcdacgqvlr ghsrqgvrcr rckanahtdc inlvqpamcp slapkksggi pllrrqktqa
      601 qvdetpaaeh nvdaiyqvlk qageirgnst nspptpptad hppapsstra sshpcsssts
      661 aphspqrrre rlnlrmksls ldspegtahv rhrprdyytt gqkestpprh sdsgsinrls
      721 pcspgqghav hkhvrgairm ssmelpdene ksysststsp cpsphtnnqn hmnppgkrvl
      781 ppnnlyvvly nfearhrdel dlkagykvtv ldksekdwwk gkclggrvgy fpsayvmrve
      841 sgqktlqvtr nlqltdqitl lrdqiviqvg eevdgvamvr caadvrsadh tgkegevlyk
      901 etlcplkylq ev
//
LOCUS       XP_034185607             879 aa            linear   INV 03-SEP-2025
DEFINITION  SH3 and cysteine-rich domain-containing protein isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185607
VERSION     XP_034185607.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329716.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..879
                     /product="SH3 and cysteine-rich domain-containing protein
                     isoform X3"
                     /calculated_mol_wt=98285
     Region          113..187
                     /region_name="FCH"
                     /note="Fes/CIP4, and EFC/F-BAR homology domain; pfam00611"
                     /db_xref="CDD:459868"
     Region          546..597
                     /region_name="C1_Stac"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in the SH3 and cysteine-rich domain-containing
                     protein (Stac) family; cd20817"
                     /db_xref="CDD:410367"
     Region          753..803
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(757,759,762,766,784..785,799,801..802)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     CDS             1..879
                     /gene="Stacl"
                     /coded_by="XM_034329716.2:719..3358"
                     /db_xref="GeneID:117606800"
ORIGIN      
        1 myviypadel lriyenqvrd pspvltsdrr lscqvtvipv dgnneqkpkg rkkkwrergq
       61 trkrrtgrne arshevakgn rrdkhtrtmq nsralaedal nplkdlvqpg lneplkqhna
      121 salklvqtvs efaqelgaam enhsamvrrl vdefrgrsgd mrvdsggmrr vwenllrqve
      181 adavahldla avlqqqlsrp tleasfhrkl qsrkvfahre ayeqvvakte eklqrarady
      241 krayatfltn dgggeqelkr ayfeahnayi lqlratnait eryqahclpg llgeiaevye
      301 elcglackcv agiseaaaer ageqakryqa vakeaqviap lndlqvlars laatatpskk
      361 psrrlfvapg ppeqvpmeri nqvpplrdel aptgtstlpl medlrrehdt lsqeisrlqd
      421 aldalvrmqr ksaesnlytk vaelqedism krfelgeaql ylaavqaqke lfgggeagqp
      481 dgvssrkmsn gstgstkhkw lkafkslkts spttppstdk gkqamyhavs tvvamsrksl
      541 esegghqwye ntyrkitpcd acgqvlrghs rqgvrcrrck anahtdcinl vqpamcpsla
      601 pkksggipll rrqktqaqvd etpaaehtph spqrrrerln lrmkslslds pegtahvrhr
      661 prdyyttgqk estpprhsds gsinrlspcs pgqghavhkh vrgairmssm elpdeneksy
      721 sststspcps phtnnqnhmn ppgkrvlppn nlyvvlynfe arhrdeldlk agykvtvldk
      781 sekdwwkgkc lggrvgyfps ayvmrvesgq ktlqvtrnlq ltdqitllrd qiviqvgeev
      841 dgvamvrcaa dvrsadhtgk egevlyketl cplkylqev
//
LOCUS       XP_076545931             790 aa            linear   INV 03-SEP-2025
DEFINITION  SH3 and cysteine-rich domain-containing protein isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545931
VERSION     XP_076545931.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689816.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..790
                     /product="SH3 and cysteine-rich domain-containing protein
                     isoform X4"
                     /calculated_mol_wt=87491
     Region          407..458
                     /region_name="C1_Stac"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in the SH3 and cysteine-rich domain-containing
                     protein (Stac) family; cd20817"
                     /db_xref="CDD:410367"
     Region          664..714
                     /region_name="SH3"
                     /note="Src homology 3 domains; smart00326"
                     /db_xref="CDD:214620"
     Site            order(668,670,673,677,695..696,710,712..713)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     CDS             1..790
                     /gene="Stacl"
                     /coded_by="XM_076689816.1:322..2694"
                     /db_xref="GeneID:117606800"
ORIGIN      
        1 menhsamvrr lvdefrgrsg dmrvdsggmr rvwenllrqv eadavahldl aavlqqqlsr
       61 ptleasfhrk lqsrkvfahr eayeqvvakt eeklqrarad ykrayatflt ndgggeqelk
      121 rayfeahnay ilqlratnai teryqahclp gllgeiaevy eelcglackc vagiseaaae
      181 rageqakryq avakeaqvia plndlqvlar slaatatpsk kpsrrlfvap gppeqvpmer
      241 inqvpplrde laptgtstlp lmedlrrehd tlsqeisrlq daldalvrmq rksaesnlyt
      301 kvaelqedis mkrfelgeaq lylaavqaqk elfgggeagq pdgvssrkms ngstgstkhk
      361 wlkafkslkt sspttppstd kgkqamyhav stvvamsrks lesegghqwy entyrkitpc
      421 dacgqvlrgh srqgvrcrrc kanahtdcin lvqpamcpsl apkksggipl lrrqktqaqv
      481 detpaaehnv daiyqvlkqa geirgnstns pptpptadhp papsstrass hpcssstsap
      541 hspqrrrerl nlrmkslsld spegtahvrh rprdyyttgq kestpprhsd sgsinrlspc
      601 spgqghavhk hvrgairmss melpdeneks ysststspcp sphtnnqnhm nppgkrvlpp
      661 nnlyvvlynf earhrdeldl kagykvtvld ksekdwwkgk clggrvgyfp sayvmrvesg
      721 qktlqvtrnl qltdqitllr dqiviqvgee vdgvamvrca advrsadhtg kegevlyket
      781 lcplkylqev
//
LOCUS       XP_034185608             440 aa            linear   INV 03-SEP-2025
DEFINITION  imaginal disc growth factor 4 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185608
VERSION     XP_034185608.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329717.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185608.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..440
                     /product="imaginal disc growth factor 4 isoform X1"
                     /calculated_mol_wt=48751
     Region          27..440
                     /region_name="GH18_IDGF"
                     /note="The IDGF's (imaginal disc growth factors) are a
                     family of growth factors identified in insects that
                     include at least five members, some of which are encoded
                     by genes in a tight cluster. The IDGF's have an
                     eight-stranded alpha/beta barrel fold and are...; cd02873"
                     /db_xref="CDD:119352"
     Site            order(31,64,112,152,154,156,246..247,306,414)
                     /site_type="other"
                     /note="carbohydrate binding site [chemical binding]"
                     /db_xref="CDD:119352"
     CDS             1..440
                     /gene="Idgf4"
                     /coded_by="XM_034329717.2:169..1491"
                     /db_xref="GeneID:117606801"
ORIGIN      
        1 mkvliftaia vfcvqsvltv dvhehnkvvc ywnstaferq gpgkfqledv rsalslcthl
       61 iygfagiksd tfevvplnpn ldtgagysyy klatqlkksf pelkvylsig gnadpedeth
      121 kylvvtetse arskfinsvn rlfndydfdg idlawqfppv kvkkqrgtfg slwhgikktf
      181 gygkfkddke dehrdgftil vrdlkaqlrp rmkaltiavl phinnsvyfd arllapnida
      241 vhlftfdqkt pernpqeady papiyesygr giednvdvpt sinsssrywl ehgtpgskiv
      301 vgiptyartw kltadsqisg vpplvaegpg aegphtnmpg vlsyaevcsr ltesavgrlr
      361 rvgdpskkyg syayqpydea tgndgiwvgy edpdtagnka ayvkakglgg vaiydlsldd
      421 frgvcigdkf pivrgakykl
//
LOCUS       XP_034185609             440 aa            linear   INV 03-SEP-2025
DEFINITION  imaginal disc growth factor 4 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185609
VERSION     XP_034185609.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329718.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185609.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..440
                     /product="imaginal disc growth factor 4 isoform X1"
                     /calculated_mol_wt=48751
     Region          27..440
                     /region_name="GH18_IDGF"
                     /note="The IDGF's (imaginal disc growth factors) are a
                     family of growth factors identified in insects that
                     include at least five members, some of which are encoded
                     by genes in a tight cluster. The IDGF's have an
                     eight-stranded alpha/beta barrel fold and are...; cd02873"
                     /db_xref="CDD:119352"
     Site            order(31,64,112,152,154,156,246..247,306,414)
                     /site_type="other"
                     /note="carbohydrate binding site [chemical binding]"
                     /db_xref="CDD:119352"
     CDS             1..440
                     /gene="Idgf4"
                     /coded_by="XM_034329718.2:31..1353"
                     /db_xref="GeneID:117606801"
ORIGIN      
        1 mkvliftaia vfcvqsvltv dvhehnkvvc ywnstaferq gpgkfqledv rsalslcthl
       61 iygfagiksd tfevvplnpn ldtgagysyy klatqlkksf pelkvylsig gnadpedeth
      121 kylvvtetse arskfinsvn rlfndydfdg idlawqfppv kvkkqrgtfg slwhgikktf
      181 gygkfkddke dehrdgftil vrdlkaqlrp rmkaltiavl phinnsvyfd arllapnida
      241 vhlftfdqkt pernpqeady papiyesygr giednvdvpt sinsssrywl ehgtpgskiv
      301 vgiptyartw kltadsqisg vpplvaegpg aegphtnmpg vlsyaevcsr ltesavgrlr
      361 rvgdpskkyg syayqpydea tgndgiwvgy edpdtagnka ayvkakglgg vaiydlsldd
      421 frgvcigdkf pivrgakykl
//
LOCUS       XP_034185610             434 aa            linear   INV 03-SEP-2025
DEFINITION  imaginal disc growth factor 4 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185610
VERSION     XP_034185610.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329719.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185610.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..434
                     /product="imaginal disc growth factor 4 isoform X2"
                     /calculated_mol_wt=48176
     Region          27..434
                     /region_name="GH18_IDGF"
                     /note="The IDGF's (imaginal disc growth factors) are a
                     family of growth factors identified in insects that
                     include at least five members, some of which are encoded
                     by genes in a tight cluster. The IDGF's have an
                     eight-stranded alpha/beta barrel fold and are...; cd02873"
                     /db_xref="CDD:119352"
     Site            order(31,64,112,152,154,156,246..247,300,408)
                     /site_type="other"
                     /note="carbohydrate binding site [chemical binding]"
                     /db_xref="CDD:119352"
     CDS             1..434
                     /gene="Idgf4"
                     /coded_by="XM_034329719.2:169..1473"
                     /db_xref="GeneID:117606801"
ORIGIN      
        1 mkvliftaia vfcvqsvltv dvhehnkvvc ywnstaferq gpgkfqledv rsalslcthl
       61 iygfagiksd tfevvplnpn ldtgagysyy klatqlkksf pelkvylsig gnadpedeth
      121 kylvvtetse arskfinsvn rlfndydfdg idlawqfppv kvkkqrgtfg slwhgikktf
      181 gygkfkddke dehrdgftil vrdlkaqlrp rmkaltiavl phinnsvyfd arllapnida
      241 vhlftfdqkt pernpqeady papiyesygr giednvdvpt rywlehgtpg skivvgipty
      301 artwkltads qisgvpplva egpgaegpht nmpgvlsyae vcsrltesav grlrrvgdps
      361 kkygsyayqp ydeatgndgi wvgyedpdta gnkaayvkak glggvaiydl slddfrgvci
      421 gdkfpivrga kykl
//
LOCUS       XP_034185611             190 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606802 [Osmia lignaria lignaria].
ACCESSION   XP_034185611
VERSION     XP_034185611.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329720.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..190
                     /product="uncharacterized protein LOC117606802"
                     /calculated_mol_wt=21960
     CDS             1..190
                     /gene="LOC117606802"
                     /coded_by="XM_034329720.2:171..743"
                     /db_xref="GeneID:117606802"
ORIGIN      
        1 mklvkssknn nlipksgmnn yqmtksisst ltkentkala rldenrplkn lqstnlarsl
       61 knqplpltpa kkrsslarpv rvydsfsqpr klmstnyafq nlqkttsest yeedeheetg
      121 wpirlrleqm leltlpqtkr krkkktsmml mkqqqsnkha ckdterllrv lsvnnvdqdn
      181 rynvtrcsvm
//
LOCUS       XP_034190671             432 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190671
VERSION     XP_034190671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..432
                     /product="G protein alpha q subunit isoform X1"
                     /calculated_mol_wt=50460
     Region          34..426
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,242,245,311..312,314,403..404)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,245,253,
                     257)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,239,241,245..247,249,252..253,255..258)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,251,254..255,258)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            242..245
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            243..259
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            311..314
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(395..406,408..411)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            403..405
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..432
                     /gene="Galphaq"
                     /coded_by="XM_034334780.2:2024..3322"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylmeidr vaapnylpte qdilrvrvpt
      181 tgiieypfdl eeirfsylsd leriekpdfl pteqdilrar apttgiieyp fdldsiifrm
      241 vdvggqrser rkwihcfenv tsiiflvals eydqilfese nenrmeeska lfktiitypw
      301 fqhssvilfl nkkdlleeki myshlvdyfp eydgpkqqdv parefilkvy lsanpdpdrm
      361 cyshftaatg pqrnastare filrmfvdln pdtekiiysh ftcatdteni rfvfaavkdt
      421 ilqsnlkeyn lv
//
LOCUS       XP_034190672             396 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190672
VERSION     XP_034190672.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334781.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="G protein alpha q subunit isoform X2"
                     /calculated_mol_wt=46410
     Region          34..390
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,242,245,311..312,314,367..368)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,245,253,
                     257)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,239,241,245..247,249,252..253,255..258)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,251,254..255,258)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            242..245
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            243..259
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            311..314
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(359..370,372..375)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            367..369
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..396
                     /gene="Galphaq"
                     /coded_by="XM_034334781.2:2011..3201"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylmeidr vaapnylpte qdilrvrvpt
      181 tgiieypfdl eeirfsylsd leriekpdfl pteqdilrar apttgiieyp fdldsiifrm
      241 vdvggqrser rkwihcfenv tsiiflvals eydqilfese nenrmeeska lfktiitypw
      301 fqhssvilfl nkkdlleeki myshlvdyfp eydgpqrnas tarefilrmf vdlnpdteki
      361 iyshftcatd tenirfvfaa vkdtilqsnl keynlv
//
LOCUS       XP_034190673             396 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190673
VERSION     XP_034190673.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334782.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="G protein alpha q subunit isoform X3"
                     /calculated_mol_wt=46235
     Region          34..390
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,242,245,311..312,314,367..368)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,245,253,
                     257)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,239,241,245..247,249,252..253,255..258)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,251,254..255,258)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            242..245
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            243..259
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            311..314
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(359..370,372..375)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            367..369
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..396
                     /gene="Galphaq"
                     /coded_by="XM_034334782.2:2030..3220"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylmeidr vaapnylpte qdilrvrvpt
      181 tgiieypfdl eeirfsylsd leriekpdfl pteqdilrar apttgiieyp fdldsiifrm
      241 vdvggqrser rkwihcfenv tsiiflvals eydqilfese nenrmeeska lfktiitypw
      301 fqhssvilfl nkkdlleeki myshlvdyfp eydgpkqqdv parefilkvy lsanpdpdrm
      361 cyshftaatd teniklvfca vkdtimqsal kefnla
//
LOCUS       XP_034190674             389 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034190674
VERSION     XP_034190674.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334783.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="G protein alpha q subunit isoform X4"
                     /calculated_mol_wt=45393
     Region          34..383
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,199,202,268..269,271,360..361)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,202,210,
                     214)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,196,198,202..204,206,209..210,212..215)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,208,211..212,215)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            199..202
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            200..216
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            268..271
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(352..363,365..368)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            360..362
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..389
                     /gene="Galphaq"
                     /coded_by="XM_034334783.2:2015..3184"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylsdler iekpdflpte qdilrarapt
      181 tgiieypfdl dsiifrmvdv ggqrserrkw ihcfenvtsi iflvalseyd qilfesenen
      241 rmeeskalfk tiitypwfqh ssvilflnkk dlleekimys hlvdyfpeyd gpkqqdvpar
      301 efilkvylsa npdpdrmcys hftaatgpqr nastarefil rmfvdlnpdt ekiiyshftc
      361 atdtenirfv faavkdtilq snlkeynlv
//
LOCUS       XP_034190675             389 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034190675
VERSION     XP_034190675.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334784.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="G protein alpha q subunit isoform X5"
                     /calculated_mol_wt=45478
     Region          34..383
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,199,202,268..269,271,360..361)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,202,210,
                     214)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,196,198,202..204,206,209..210,212..215)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,208,211..212,215)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            199..202
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            200..216
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            268..271
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(352..363,365..368)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            360..362
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..389
                     /gene="Galphaq"
                     /coded_by="XM_034334784.2:2019..3188"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylmeidr vaapnylpte qdilrvrvpt
      181 tgiieypfdl eeirfrmvdv ggqrserrkw ihcfenvtsi iflvalseyd qilfesenen
      241 rmeeskalfk tiitypwfqh ssvilflnkk dlleekimys hlvdyfpeyd gpkqqdvpar
      301 efilkvylsa npdpdrmcys hftaatgpqr nastarefil rmfvdlnpdt ekiiyshftc
      361 atdtenirfv faavkdtilq snlkeynlv
//
LOCUS       XP_034190677             353 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034190677
VERSION     XP_034190677.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334786.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="G protein alpha q subunit isoform X6"
                     /calculated_mol_wt=41343
     Region          34..347
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,199,202,268..269,271,324..325)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,202,210,
                     214)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,196,198,202..204,206,209..210,212..215)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,208,211..212,215)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            199..202
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            200..216
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            268..271
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(316..327,329..332)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            324..326
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..353
                     /gene="Galphaq"
                     /coded_by="XM_034334786.2:1994..3055"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylsdler iekpdflpte qdilrarapt
      181 tgiieypfdl dsiifrmvdv ggqrserrkw ihcfenvtsi iflvalseyd qilfesenen
      241 rmeeskalfk tiitypwfqh ssvilflnkk dlleekimys hlvdyfpeyd gpqrnastar
      301 efilrmfvdl npdtekiiys hftcatdten irfvfaavkd tilqsnlkey nlv
//
LOCUS       XP_034190676             353 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034190676
VERSION     XP_034190676.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334785.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="G protein alpha q subunit isoform X7"
                     /calculated_mol_wt=41429
     Region          34..347
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,199,202,268..269,271,324..325)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,202,210,
                     214)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,196,198,202..204,206,209..210,212..215)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,208,211..212,215)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            199..202
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            200..216
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            268..271
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(316..327,329..332)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            324..326
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..353
                     /gene="Galphaq"
                     /coded_by="XM_034334785.2:2000..3061"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylmeidr vaapnylpte qdilrvrvpt
      181 tgiieypfdl eeirfrmvdv ggqrserrkw ihcfenvtsi iflvalseyd qilfesenen
      241 rmeeskalfk tiitypwfqh ssvilflnkk dlleekimys hlvdyfpeyd gpqrnastar
      301 efilrmfvdl npdtekiiys hftcatdten irfvfaavkd tilqsnlkey nlv
//
LOCUS       XP_034190678             353 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034190678
VERSION     XP_034190678.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334787.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="G protein alpha q subunit isoform X8"
                     /calculated_mol_wt=41168
     Region          34..347
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,199,202,268..269,271,324..325)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,202,210,
                     214)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,196,198,202..204,206,209..210,212..215)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,208,211..212,215)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            199..202
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            200..216
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            268..271
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(316..327,329..332)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            324..326
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..353
                     /gene="Galphaq"
                     /coded_by="XM_034334787.2:2019..3080"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylsdler iekpdflpte qdilrarapt
      181 tgiieypfdl dsiifrmvdv ggqrserrkw ihcfenvtsi iflvalseyd qilfesenen
      241 rmeeskalfk tiitypwfqh ssvilflnkk dlleekimys hlvdyfpeyd gpkqqdvpar
      301 efilkvylsa npdpdrmcys hftaatdten iklvfcavkd timqsalkef nla
//
LOCUS       XP_034190679             353 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034190679
VERSION     XP_034190679.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334788.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="G protein alpha q subunit isoform X9"
                     /calculated_mol_wt=41253
     Region          34..347
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,199,202,268..269,271,324..325)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,202,210,
                     214)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,196,198,202..204,206,209..210,212..215)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,208,211..212,215)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            199..202
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            200..216
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            268..271
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(316..327,329..332)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            324..326
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..353
                     /gene="Galphaq"
                     /coded_by="XM_034334788.2:2024..3085"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylmeidr vaapnylpte qdilrvrvpt
      181 tgiieypfdl eeirfrmvdv ggqrserrkw ihcfenvtsi iflvalseyd qilfesenen
      241 rmeeskalfk tiitypwfqh ssvilflnkk dlleekimys hlvdyfpeyd gpkqqdvpar
      301 efilkvylsa npdpdrmcys hftaatdten iklvfcavkd timqsalkef nla
//
LOCUS       XP_034190680             396 aa            linear   INV 03-SEP-2025
DEFINITION  G protein alpha q subunit isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_034190680
VERSION     XP_034190680.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334789.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="G protein alpha q subunit isoform X10"
                     /calculated_mol_wt=46311
     Region          34..390
                     /region_name="G-alpha"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); cd00066"
                     /db_xref="CDD:206639"
     Site            order(40,45..47,180,242,245,311..312,314,367..368)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206639"
     Site            40..47
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206639"
     Site            order(41..43,71,75,79,82,85..86,104,107,148,177,245,253,
                     257)
                     /site_type="active"
                     /note="GoLoco binding site [active]"
                     /db_xref="CDD:206639"
     Site            175..183
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206639"
     Site            180
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206639"
     Site            order(181,183,185,239,241,245..247,249,252..253,255..258)
                     /site_type="other"
                     /note="beta - gamma complex interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            order(183,251,254..255,258)
                     /site_type="other"
                     /note="adenylyl cyclase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206639"
     Site            242..245
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206639"
     Site            243..259
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206639"
     Site            311..314
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206639"
     Site            order(359..370,372..375)
                     /site_type="active"
                     /note="putative receptor binding site [active]"
                     /db_xref="CDD:206639"
     Site            367..369
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206639"
     CDS             1..396
                     /gene="Galphaq"
                     /coded_by="XM_034334789.2:2021..3211"
                     /db_xref="GeneID:117608975"
ORIGIN      
        1 macclseeak eqkrinqeie rqlrkdkrda rrelkllllg tgesgkstfi kqmriihgsg
       61 ysdddkrgfi klvyqnifma mqsmiramel lkiqygessn iekaelvrsv dfetvtafes
      121 pyveaikdlw adagiqecyd rrreyqltds akyylmeidr vaapnylpte qdilrvrvpt
      181 tgiieypfdl eeirfsylsd leriekpdfl pteqdilrar apttgiieyp fdldsiifrm
      241 vdvggqrser rkwihcfenv tsiiflvals eydqilfese nenrmeeska lfktiitypw
      301 fqhssvilfl nkkdlleeki myshlvdyfp eydgpqrnas tarefilrmf vdlnpdteki
      361 iyshftcatd teniklvfca vkdtimqsal kefnla
//
LOCUS       XP_076545932            1606 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545932
VERSION     XP_076545932.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689817.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1606
                     /product="uncharacterized protein LOC117608969 isoform X1"
                     /calculated_mol_wt=178129
     Region          171..>226
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..1606
                     /gene="LOC117608969"
                     /coded_by="XM_076689817.1:401..5221"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 mlclkkrrty sftqgvctel prrtkednrr yqgslnmait tlprknrtre erpsrmiltv
       61 tedtpadmla gsmellvqlp rdhhvqtqrv tvqrstpmmd llvqiatahk laassytlqa
      121 igergmvlph qpntpigald alqvkllpkq gtfvprkakq anqpfettfr lqvhlprnql
      181 yvsrvspkmn lgeildevcr eknldknkye lrhpanleet ldlsltlqdy hlqevtlyak
      241 qgrtlgsals tqdimalqrq eerrrqqakq gvfsfykksk esslstdslg grsasparsd
      301 etgrsssplq pparpqrkrr paprppvpaq aegteessgd sskdkvvish srnssdssgy
      361 heasvlsdnp estgrlpetl prrnkvptet prklaqtsqs skslgnlatv pgtlshgiss
      421 tsisstglrk kraappppvt rplssaissq glerivdsee sltsdmdpsk ppsdisassk
      481 ansdieerpk assdigvtti ptklsstvdf tksdcpnada elkssksdie trhrsgfvnv
      541 kssntvagnp tpiedapvda rvsrkrvgep vplpkprkaa prpvvpkrkl apsfppsntl
      601 ssgsdnverl lnagtnpsid vsstdassev vqsstnfeek letasncsts esevfltpgv
      661 dqseatfdyn svilkeneve sltavqtdnk eeevcsskek nienpstakg ssnqekklca
      721 vistipkcev lnalnvdntk tqnkekegik dpkrtriprt asksndfetn slerqrrhlt
      781 sqleditfal nlnvasnvgl lsdrsekfqg nvqqleekqn ncsqgvgggk shpemnasdk
      841 efsetdvllq kvsdtlsnsl pvsnekaste tkskinipkd tdqknkdids adssnstnam
      901 nltansqqdt sdyvsaaded lsiaeweyql pappsafrds sspvfdnfde itpsseitkd
      961 skikepefsg tndpkdslke hhpkkqeske tlkkeathek vfkrvessmk kevinelenk
     1021 isslpqtakd vdsrrassns sapkvapvdn tlsnftitty trqknlnife eveqqsrkkn
     1081 tderfvrsfa tlsrnqdrdl tdfssgtlqe kkqepkinrt etlqrwqtnn eksniqrsks
     1141 ylsvcdkakf qggtcedddk nsaelemdag mkkatsinsl nstavrsnek fsrwrdnilk
     1201 rqeeptkesq lqsvqvlksi lpqlksaqqa eenvskelnn tvlqektrpe tttsnehsme
     1261 lnvvstrdsq aapsnkkpef kklpsetsak rytysgppai slgswsdrpn invqikkdtd
     1321 ykfgasksnr tvvdingske etngsnmedt vksqrskfei adrrepnelt kklithttas
     1381 gfkkpvlnkv nsesktvsdk pvvtgvelkk sflenkqdds vidttpmnfk elskafdqev
     1441 ylrpkpkrin vnrrsdlqle rdsskqngha nsdqcstngf qdhskvkrft tivgmqpqnq
     1501 tnlafrnain gkcnppmpvv kgfkisnvki egsqenqnnl lkerskelng dsqppkpptm
     1561 pvitgvtlks trpksmpvqm dsrdmllesi rnfggrenlk naaerc
//
LOCUS       XP_034190660            1606 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190660
VERSION     XP_034190660.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190660.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1606
                     /product="uncharacterized protein LOC117608969 isoform X1"
                     /calculated_mol_wt=178129
     Region          171..>226
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..1606
                     /gene="LOC117608969"
                     /coded_by="XM_034334769.2:335..5155"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 mlclkkrrty sftqgvctel prrtkednrr yqgslnmait tlprknrtre erpsrmiltv
       61 tedtpadmla gsmellvqlp rdhhvqtqrv tvqrstpmmd llvqiatahk laassytlqa
      121 igergmvlph qpntpigald alqvkllpkq gtfvprkakq anqpfettfr lqvhlprnql
      181 yvsrvspkmn lgeildevcr eknldknkye lrhpanleet ldlsltlqdy hlqevtlyak
      241 qgrtlgsals tqdimalqrq eerrrqqakq gvfsfykksk esslstdslg grsasparsd
      301 etgrsssplq pparpqrkrr paprppvpaq aegteessgd sskdkvvish srnssdssgy
      361 heasvlsdnp estgrlpetl prrnkvptet prklaqtsqs skslgnlatv pgtlshgiss
      421 tsisstglrk kraappppvt rplssaissq glerivdsee sltsdmdpsk ppsdisassk
      481 ansdieerpk assdigvtti ptklsstvdf tksdcpnada elkssksdie trhrsgfvnv
      541 kssntvagnp tpiedapvda rvsrkrvgep vplpkprkaa prpvvpkrkl apsfppsntl
      601 ssgsdnverl lnagtnpsid vsstdassev vqsstnfeek letasncsts esevfltpgv
      661 dqseatfdyn svilkeneve sltavqtdnk eeevcsskek nienpstakg ssnqekklca
      721 vistipkcev lnalnvdntk tqnkekegik dpkrtriprt asksndfetn slerqrrhlt
      781 sqleditfal nlnvasnvgl lsdrsekfqg nvqqleekqn ncsqgvgggk shpemnasdk
      841 efsetdvllq kvsdtlsnsl pvsnekaste tkskinipkd tdqknkdids adssnstnam
      901 nltansqqdt sdyvsaaded lsiaeweyql pappsafrds sspvfdnfde itpsseitkd
      961 skikepefsg tndpkdslke hhpkkqeske tlkkeathek vfkrvessmk kevinelenk
     1021 isslpqtakd vdsrrassns sapkvapvdn tlsnftitty trqknlnife eveqqsrkkn
     1081 tderfvrsfa tlsrnqdrdl tdfssgtlqe kkqepkinrt etlqrwqtnn eksniqrsks
     1141 ylsvcdkakf qggtcedddk nsaelemdag mkkatsinsl nstavrsnek fsrwrdnilk
     1201 rqeeptkesq lqsvqvlksi lpqlksaqqa eenvskelnn tvlqektrpe tttsnehsme
     1261 lnvvstrdsq aapsnkkpef kklpsetsak rytysgppai slgswsdrpn invqikkdtd
     1321 ykfgasksnr tvvdingske etngsnmedt vksqrskfei adrrepnelt kklithttas
     1381 gfkkpvlnkv nsesktvsdk pvvtgvelkk sflenkqdds vidttpmnfk elskafdqev
     1441 ylrpkpkrin vnrrsdlqle rdsskqngha nsdqcstngf qdhskvkrft tivgmqpqnq
     1501 tnlafrnain gkcnppmpvv kgfkisnvki egsqenqnnl lkerskelng dsqppkpptm
     1561 pvitgvtlks trpksmpvqm dsrdmllesi rnfggrenlk naaerc
//
LOCUS       XP_034190661            1576 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190661
VERSION     XP_034190661.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190661.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1576
                     /product="uncharacterized protein LOC117608969 isoform X2"
                     /calculated_mol_wt=174530
     Region          141..>196
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..1576
                     /gene="LOC117608969"
                     /coded_by="XM_034334770.2:141..4871"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 mgikmgtkir rfirrlgtre erpsrmiltv tedtpadmla gsmellvqlp rdhhvqtqrv
       61 tvqrstpmmd llvqiatahk laassytlqa igergmvlph qpntpigald alqvkllpkq
      121 gtfvprkakq anqpfettfr lqvhlprnql yvsrvspkmn lgeildevcr eknldknkye
      181 lrhpanleet ldlsltlqdy hlqevtlyak qgrtlgsals tqdimalqrq eerrrqqakq
      241 gvfsfykksk esslstdslg grsasparsd etgrsssplq pparpqrkrr paprppvpaq
      301 aegteessgd sskdkvvish srnssdssgy heasvlsdnp estgrlpetl prrnkvptet
      361 prklaqtsqs skslgnlatv pgtlshgiss tsisstglrk kraappppvt rplssaissq
      421 glerivdsee sltsdmdpsk ppsdisassk ansdieerpk assdigvtti ptklsstvdf
      481 tksdcpnada elkssksdie trhrsgfvnv kssntvagnp tpiedapvda rvsrkrvgep
      541 vplpkprkaa prpvvpkrkl apsfppsntl ssgsdnverl lnagtnpsid vsstdassev
      601 vqsstnfeek letasncsts esevfltpgv dqseatfdyn svilkeneve sltavqtdnk
      661 eeevcsskek nienpstakg ssnqekklca vistipkcev lnalnvdntk tqnkekegik
      721 dpkrtriprt asksndfetn slerqrrhlt sqleditfal nlnvasnvgl lsdrsekfqg
      781 nvqqleekqn ncsqgvgggk shpemnasdk efsetdvllq kvsdtlsnsl pvsnekaste
      841 tkskinipkd tdqknkdids adssnstnam nltansqqdt sdyvsaaded lsiaeweyql
      901 pappsafrds sspvfdnfde itpsseitkd skikepefsg tndpkdslke hhpkkqeske
      961 tlkkeathek vfkrvessmk kevinelenk isslpqtakd vdsrrassns sapkvapvdn
     1021 tlsnftitty trqknlnife eveqqsrkkn tderfvrsfa tlsrnqdrdl tdfssgtlqe
     1081 kkqepkinrt etlqrwqtnn eksniqrsks ylsvcdkakf qggtcedddk nsaelemdag
     1141 mkkatsinsl nstavrsnek fsrwrdnilk rqeeptkesq lqsvqvlksi lpqlksaqqa
     1201 eenvskelnn tvlqektrpe tttsnehsme lnvvstrdsq aapsnkkpef kklpsetsak
     1261 rytysgppai slgswsdrpn invqikkdtd ykfgasksnr tvvdingske etngsnmedt
     1321 vksqrskfei adrrepnelt kklithttas gfkkpvlnkv nsesktvsdk pvvtgvelkk
     1381 sflenkqdds vidttpmnfk elskafdqev ylrpkpkrin vnrrsdlqle rdsskqngha
     1441 nsdqcstngf qdhskvkrft tivgmqpqnq tnlafrnain gkcnppmpvv kgfkisnvki
     1501 egsqenqnnl lkerskelng dsqppkpptm pvitgvtlks trpksmpvqm dsrdmllesi
     1561 rnfggrenlk naaerc
//
LOCUS       XP_034190663            1567 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190663
VERSION     XP_034190663.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334772.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190663.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1567
                     /product="uncharacterized protein LOC117608969 isoform X3"
                     /calculated_mol_wt=173535
     Region          132..>187
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..1567
                     /gene="LOC117608969"
                     /coded_by="XM_034334772.2:16..4719"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 mqiprhtrtr eerpsrmilt vtedtpadml agsmellvql prdhhvqtqr vtvqrstpmm
       61 dllvqiatah klaassytlq aigergmvlp hqpntpigal dalqvkllpk qgtfvprkak
      121 qanqpfettf rlqvhlprnq lyvsrvspkm nlgeildevc reknldknky elrhpanlee
      181 tldlsltlqd yhlqevtlya kqgrtlgsal stqdimalqr qeerrrqqak qgvfsfykks
      241 kesslstdsl ggrsaspars detgrssspl qpparpqrkr rpaprppvpa qaegteessg
      301 dsskdkvvis hsrnssdssg yheasvlsdn pestgrlpet lprrnkvpte tprklaqtsq
      361 sskslgnlat vpgtlshgis stsisstglr kkraappppv trplssaiss qglerivdse
      421 esltsdmdps kppsdisass kansdieerp kassdigvtt iptklsstvd ftksdcpnad
      481 aelkssksdi etrhrsgfvn vkssntvagn ptpiedapvd arvsrkrvge pvplpkprka
      541 aprpvvpkrk lapsfppsnt lssgsdnver llnagtnpsi dvsstdasse vvqsstnfee
      601 kletasncst sesevfltpg vdqseatfdy nsvilkenev esltavqtdn keeevcsske
      661 knienpstak gssnqekklc avistipkce vlnalnvdnt ktqnkekegi kdpkrtripr
      721 tasksndfet nslerqrrhl tsqleditfa lnlnvasnvg llsdrsekfq gnvqqleekq
      781 nncsqgvggg kshpemnasd kefsetdvll qkvsdtlsns lpvsnekast etkskinipk
      841 dtdqknkdid sadssnstna mnltansqqd tsdyvsaade dlsiaeweyq lpappsafrd
      901 ssspvfdnfd eitpsseitk dskikepefs gtndpkdslk ehhpkkqesk etlkkeathe
      961 kvfkrvessm kkevinelen kisslpqtak dvdsrrassn ssapkvapvd ntlsnftitt
     1021 ytrqknlnif eeveqqsrkk ntderfvrsf atlsrnqdrd ltdfssgtlq ekkqepkinr
     1081 tetlqrwqtn neksniqrsk sylsvcdkak fqggtceddd knsaelemda gmkkatsins
     1141 lnstavrsne kfsrwrdnil krqeeptkes qlqsvqvlks ilpqlksaqq aeenvskeln
     1201 ntvlqektrp etttsnehsm elnvvstrds qaapsnkkpe fkklpsetsa krytysgppa
     1261 islgswsdrp ninvqikkdt dykfgasksn rtvvdingsk eetngsnmed tvksqrskfe
     1321 iadrrepnel tkklithtta sgfkkpvlnk vnsesktvsd kpvvtgvelk ksflenkqdd
     1381 svidttpmnf kelskafdqe vylrpkpkri nvnrrsdlql erdsskqngh ansdqcstng
     1441 fqdhskvkrf ttivgmqpqn qtnlafrnai ngkcnppmpv vkgfkisnvk iegsqenqnn
     1501 llkerskeln gdsqppkppt mpvitgvtlk strpksmpvq mdsrdmlles irnfggrenl
     1561 knaaerc
//
LOCUS       XP_034190664            1551 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190664
VERSION     XP_034190664.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190664.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1551
                     /product="uncharacterized protein LOC117608969 isoform X4"
                     /calculated_mol_wt=171503
     Region          116..>171
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..1551
                     /gene="LOC117608969"
                     /coded_by="XM_034334773.2:379..5034"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 miltvtedtp admlagsmel lvqlprdhhv qtqrvtvqrs tpmmdllvqi atahklaass
       61 ytlqaigerg mvlphqpntp igaldalqvk llpkqgtfvp rkakqanqpf ettfrlqvhl
      121 prnqlyvsrv spkmnlgeil devcreknld knkyelrhpa nleetldlsl tlqdyhlqev
      181 tlyakqgrtl gsalstqdim alqrqeerrr qqakqgvfsf ykkskessls tdslggrsas
      241 parsdetgrs ssplqpparp qrkrrpaprp pvpaqaegte essgdsskdk vvishsrnss
      301 dssgyheasv lsdnpestgr lpetlprrnk vptetprkla qtsqsskslg nlatvpgtls
      361 hgisstsiss tglrkkraap pppvtrplss aissqgleri vdseesltsd mdpskppsdi
      421 sasskansdi eerpkassdi gvttiptkls stvdftksdc pnadaelkss ksdietrhrs
      481 gfvnvkssnt vagnptpied apvdarvsrk rvgepvplpk prkaaprpvv pkrklapsfp
      541 psntlssgsd nverllnagt npsidvsstd assevvqsst nfeekletas ncstsesevf
      601 ltpgvdqsea tfdynsvilk enevesltav qtdnkeeevc sskeknienp stakgssnqe
      661 kklcavisti pkcevlnaln vdntktqnke kegikdpkrt riprtasksn dfetnslerq
      721 rrhltsqled itfalnlnva snvgllsdrs ekfqgnvqql eekqnncsqg vgggkshpem
      781 nasdkefset dvllqkvsdt lsnslpvsne kastetkski nipkdtdqkn kdidsadssn
      841 stnamnltan sqqdtsdyvs aadedlsiae weyqlpapps afrdssspvf dnfdeitpss
      901 eitkdskike pefsgtndpk dslkehhpkk qesketlkke athekvfkrv essmkkevin
      961 elenkisslp qtakdvdsrr assnssapkv apvdntlsnf tittytrqkn lnifeeveqq
     1021 srkkntderf vrsfatlsrn qdrdltdfss gtlqekkqep kinrtetlqr wqtnneksni
     1081 qrsksylsvc dkakfqggtc edddknsael emdagmkkat sinslnstav rsnekfsrwr
     1141 dnilkrqeep tkesqlqsvq vlksilpqlk saqqaeenvs kelnntvlqe ktrpetttsn
     1201 ehsmelnvvs trdsqaapsn kkpefkklps etsakrytys gppaislgsw sdrpninvqi
     1261 kkdtdykfga sksnrtvvdi ngskeetngs nmedtvksqr skfeiadrre pneltkklit
     1321 httasgfkkp vlnkvnsesk tvsdkpvvtg velkksflen kqddsvidtt pmnfkelska
     1381 fdqevylrpk pkrinvnrrs dlqlerdssk qnghansdqc stngfqdhsk vkrfttivgm
     1441 qpqnqtnlaf rnaingkcnp pmpvvkgfki snvkiegsqe nqnnllkers kelngdsqpp
     1501 kpptmpvitg vtlkstrpks mpvqmdsrdm llesirnfgg renlknaaer c
//
LOCUS       XP_034190665            1429 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190665
VERSION     XP_034190665.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334774.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190665.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1429
                     /product="uncharacterized protein LOC117608969 isoform X5"
                     /calculated_mol_wt=157685
     CDS             1..1429
                     /gene="LOC117608969"
                     /coded_by="XM_034334774.2:441..4730"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 mprvtfillv laeastlall syaflgprri slrafttpnl eetldlsltl qdyhlqevtl
       61 yakqgrtlgs alstqdimal qrqeerrrqq akqgvfsfyk kskesslstd slggrsaspa
      121 rsdetgrsss plqpparpqr krrpaprppv paqaegtees sgdsskdkvv ishsrnssds
      181 sgyheasvls dnpestgrlp etlprrnkvp tetprklaqt sqsskslgnl atvpgtlshg
      241 isstsisstg lrkkraappp pvtrplssai ssqglerivd seesltsdmd pskppsdisa
      301 sskansdiee rpkassdigv ttiptklsst vdftksdcpn adaelkssks dietrhrsgf
      361 vnvkssntva gnptpiedap vdarvsrkrv gepvplpkpr kaaprpvvpk rklapsfpps
      421 ntlssgsdnv erllnagtnp sidvsstdas sevvqsstnf eekletasnc stsesevflt
      481 pgvdqseatf dynsvilken evesltavqt dnkeeevcss keknienpst akgssnqekk
      541 lcavistipk cevlnalnvd ntktqnkeke gikdpkrtri prtasksndf etnslerqrr
      601 hltsqledit falnlnvasn vgllsdrsek fqgnvqqlee kqnncsqgvg ggkshpemna
      661 sdkefsetdv llqkvsdtls nslpvsneka stetkskini pkdtdqknkd idsadssnst
      721 namnltansq qdtsdyvsaa dedlsiaewe yqlpappsaf rdssspvfdn fdeitpssei
      781 tkdskikepe fsgtndpkds lkehhpkkqe sketlkkeat hekvfkrves smkkevinel
      841 enkisslpqt akdvdsrras snssapkvap vdntlsnfti ttytrqknln ifeeveqqsr
      901 kkntderfvr sfatlsrnqd rdltdfssgt lqekkqepki nrtetlqrwq tnneksniqr
      961 sksylsvcdk akfqggtced ddknsaelem dagmkkatsi nslnstavrs nekfsrwrdn
     1021 ilkrqeeptk esqlqsvqvl ksilpqlksa qqaeenvske lnntvlqekt rpetttsneh
     1081 smelnvvstr dsqaapsnkk pefkklpset sakrytysgp paislgswsd rpninvqikk
     1141 dtdykfgask snrtvvding skeetngsnm edtvksqrsk feiadrrepn eltkklitht
     1201 tasgfkkpvl nkvnsesktv sdkpvvtgve lkksflenkq ddsvidttpm nfkelskafd
     1261 qevylrpkpk rinvnrrsdl qlerdsskqn ghansdqcst ngfqdhskvk rfttivgmqp
     1321 qnqtnlafrn aingkcnppm pvvkgfkisn vkiegsqenq nnllkerske lngdsqppkp
     1381 ptmpvitgvt lkstrpksmp vqmdsrdmll esirnfggre nlknaaerc
//
LOCUS       XP_034190666            1352 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190666
VERSION     XP_034190666.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334775.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190666.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1352
                     /product="uncharacterized protein LOC117608969 isoform X6"
                     /calculated_mol_wt=149135
     CDS             1..1352
                     /gene="LOC117608969"
                     /coded_by="XM_034334775.2:549..4607"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 malqrqeerr rqqakqgvfs fykkskessl stdslggrsa sparsdetgr sssplqppar
       61 pqrkrrpapr ppvpaqaegt eessgdsskd kvvishsrns sdssgyheas vlsdnpestg
      121 rlpetlprrn kvptetprkl aqtsqssksl gnlatvpgtl shgisstsis stglrkkraa
      181 ppppvtrpls saissqgler ivdseeslts dmdpskppsd isasskansd ieerpkassd
      241 igvttiptkl sstvdftksd cpnadaelks sksdietrhr sgfvnvkssn tvagnptpie
      301 dapvdarvsr krvgepvplp kprkaaprpv vpkrklapsf ppsntlssgs dnverllnag
      361 tnpsidvsst dassevvqss tnfeekleta sncstsesev fltpgvdqse atfdynsvil
      421 keneveslta vqtdnkeeev csskeknien pstakgssnq ekklcavist ipkcevlnal
      481 nvdntktqnk ekegikdpkr triprtasks ndfetnsler qrrhltsqle ditfalnlnv
      541 asnvgllsdr sekfqgnvqq leekqnncsq gvgggkshpe mnasdkefse tdvllqkvsd
      601 tlsnslpvsn ekastetksk inipkdtdqk nkdidsadss nstnamnlta nsqqdtsdyv
      661 saadedlsia eweyqlpapp safrdssspv fdnfdeitps seitkdskik epefsgtndp
      721 kdslkehhpk kqesketlkk eathekvfkr vessmkkevi nelenkissl pqtakdvdsr
      781 rassnssapk vapvdntlsn ftittytrqk nlnifeeveq qsrkkntder fvrsfatlsr
      841 nqdrdltdfs sgtlqekkqe pkinrtetlq rwqtnneksn iqrsksylsv cdkakfqggt
      901 cedddknsae lemdagmkka tsinslnsta vrsnekfsrw rdnilkrqee ptkesqlqsv
      961 qvlksilpql ksaqqaeenv skelnntvlq ektrpettts nehsmelnvv strdsqaaps
     1021 nkkpefkklp setsakryty sgppaislgs wsdrpninvq ikkdtdykfg asksnrtvvd
     1081 ingskeetng snmedtvksq rskfeiadrr epneltkkli thttasgfkk pvlnkvnses
     1141 ktvsdkpvvt gvelkksfle nkqddsvidt tpmnfkelsk afdqevylrp kpkrinvnrr
     1201 sdlqlerdss kqnghansdq cstngfqdhs kvkrfttivg mqpqnqtnla frnaingkcn
     1261 ppmpvvkgfk isnvkiegsq enqnnllker skelngdsqp pkpptmpvit gvtlkstrpk
     1321 smpvqmdsrd mllesirnfg grenlknaae rc
//
LOCUS       XP_034190667            1334 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608969 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190667
VERSION     XP_034190667.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190667.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1334
                     /product="uncharacterized protein LOC117608969 isoform X7"
                     /calculated_mol_wt=148585
     Region          171..>226
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     CDS             1..1334
                     /gene="LOC117608969"
                     /coded_by="XM_034334776.2:393..4397"
                     /db_xref="GeneID:117608969"
ORIGIN      
        1 mlclkkrrty sftqgvctel prrtkednrr yqgslnmait tlprknrtre erpsrmiltv
       61 tedtpadmla gsmellvqlp rdhhvqtqrv tvqrstpmmd llvqiatahk laassytlqa
      121 igergmvlph qpntpigald alqvkllpkq gtfvprkakq anqpfettfr lqvhlprnql
      181 yvsrvspkmn lgeildevcr eknldknkye lrhpanleet ldlsltlqdy hlqevtlyak
      241 qgrtlgsals tqdimalqrq eerrrqqakq gvfsfykksk esslstdslg grsasparsd
      301 etgrsssplq pparpqrkrr paprppvpaq aegteessgd sskdkvvish srnssdssgy
      361 heasvlsdnp estgrlpetl prrnkvptet prklaqtsqs skslgnlatv pgtlshgiss
      421 tsisstglrk kraappppvt rplssaissq glerivdsee sltsdmdpsk ppsdisassk
      481 ansdieerpk assdigvtti ptklsstvdf tksdcpnada elkssksdie trhrsgfvnv
      541 kssntvagnp tpiedapvda rvsrkrdkef setdvllqkv sdtlsnslpv snekastetk
      601 skinipkdtd qknkdidsad ssnstnamnl tansqqdtsd yvsaadedls iaeweyqlpa
      661 ppsafrdsss pvfdnfdeit psseitkdsk ikepefsgtn dpkdslkehh pkkqesketl
      721 kkeathekvf krvessmkke vinelenkis slpqtakdvd srrassnssa pkvapvdntl
      781 snftittytr qknlnifeev eqqsrkkntd erfvrsfatl srnqdrdltd fssgtlqekk
      841 qepkinrtet lqrwqtnnek sniqrsksyl svcdkakfqg gtcedddkns aelemdagmk
      901 katsinslns tavrsnekfs rwrdnilkrq eeptkesqlq svqvlksilp qlksaqqaee
      961 nvskelnntv lqektrpett tsnehsmeln vvstrdsqaa psnkkpefkk lpsetsakry
     1021 tysgppaisl gswsdrpnin vqikkdtdyk fgasksnrtv vdingskeet ngsnmedtvk
     1081 sqrskfeiad rrepneltkk lithttasgf kkpvlnkvns esktvsdkpv vtgvelkksf
     1141 lenkqddsvi dttpmnfkel skafdqevyl rpkpkrinvn rrsdlqlerd sskqnghans
     1201 dqcstngfqd hskvkrftti vgmqpqnqtn lafrnaingk cnppmpvvkg fkisnvkieg
     1261 sqenqnnllk erskelngds qppkpptmpv itgvtlkstr pksmpvqmds rdmllesirn
     1321 fggrenlkna aerc
//
LOCUS       XP_034190668             356 aa            linear   INV 03-SEP-2025
DEFINITION  xyloside xylosyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034190668
VERSION     XP_034190668.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334777.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..356
                     /product="xyloside xylosyltransferase"
                     /calculated_mol_wt=40568
     Region          83..>319
                     /region_name="Glyco_tranf_GTA_type"
                     /note="Glycosyltransferase family A (GT-A) includes
                     diverse families of glycosyl transferases with a common
                     GT-A type structural fold; cl11394"
                     /db_xref="CDD:472172"
     CDS             1..356
                     /gene="Xxylt"
                     /coded_by="XM_034334777.2:277..1347"
                     /db_xref="GeneID:117608971"
ORIGIN      
        1 mvllrkvlin ltllavllvl fycyqtsngi hwvlhtyeqe nisttesnng entssatfva
       61 sekmyynvwc iftkvasnsp mkrkfeifad sllrlssvdi aihvitddds kqvaeqvlkg
      121 illstgkfmk vqyydvhela lqlediisvm sphfsskpgt yysdalffls lglhriapae
      181 qnvaamfdad tkfrkdikel feefnsfgee alfglapelt pvyrhvlyly rnkhpdtlfg
      241 epassggypg ynsgvvlfnl nklrnssiyn kivkresvns itekyhfkgh lgdqdfytll
      301 gmerpeliht idcgwnrqlc twwrdrgyad vfgshskcds etklwhgncn tpipdd
//
LOCUS       XP_034190670             186 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor
            3 [Osmia lignaria lignaria].
ACCESSION   XP_034190670
VERSION     XP_034190670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334779.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..186
                     /product="NADH dehydrogenase [ubiquinone] 1 alpha
                     subcomplex assembly factor 3"
                     /calculated_mol_wt=20627
     Region          47..161
                     /region_name="Mth938-like"
                     /note="Mth938-like domain. The members of this family
                     include: Mth938, 2P1, Xcr35, Rpa2829, and several
                     uncharacterized sequences. Mth938 is a hypothetical
                     protein encoded by the Methanobacterium
                     thermoautotrophicum (Mth) genome. This protein
                     crystallizes as a...; cl00182"
                     /db_xref="CDD:469644"
     CDS             1..186
                     /gene="LOC117608972"
                     /coded_by="XM_034334779.2:97..657"
                     /db_xref="GeneID:117608972"
ORIGIN      
        1 mvlnnkllti nqllqnvrrf hcskvkkaye gsgkstmhii nvagpklfit kcssigfqls
       61 dnsmvvgpla ifartilcwn iacaknisea nlslftalep kldvlilgle tkydqktlna
      121 ikkitsekni nteilpvdqa cgiynflcee gryvaaglip plpeethfyk klttnlkslq
      181 kdklie
//
LOCUS       XP_034185350             821 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185350
VERSION     XP_034185350.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329459.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..821
                     /product="ATP-binding cassette sub-family G member 5"
                     /calculated_mol_wt=90841
     Region          244..>648
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..821
                     /gene="LOC117606679"
                     /coded_by="XM_034329459.2:617..3082"
                     /db_xref="GeneID:117606679"
ORIGIN      
        1 mgseaweler rysvpgaldt rgleppased lhawsiyrqn lnsdftdsal gsaeksplpy
       61 gnfqlrdstv qsilrhpryg pksplasnsy tylkfglprv lppssrnrdg ssgydsseeg
      121 rrvshagtsg hgttsamrsa rsdpdfrhvh avprehahsq ltvsrenprl rsaseanllq
      181 ggaknnrrhs iapidpgygv heprghgilg pesyalplrp vpclqhphlq lrgveasgsd
      241 glpllrgitl eagatevlai mattekegkl ivetiagrkr ikrgdillng rsvssrslrs
      301 rvaylstecs lspgltaqqt lsfymllrgg pntskleaea ilqelgleat khclvnsltt
      361 searrlalac rllqdshila ldrpthgldi fdafflvdyl rqwanrgqrl vvltlhppty
      421 eiltmvskva ltsggrimys gprrdmlpyf alaefpcppf knpsdyyldl vtlddlsaea
      481 mlessqridh laelartrlp plcdpgppga lppsisspni ftqiyalllr tliysqpwtl
      541 trllrkivis aslsillgai fwdvagdsnl hlrdrvgfhy aslgilfwpl tlmaicevad
      601 crpnverdik dglygrfiyi fmellcgils wcivyliyla pgyamsglhl vpdenltslw
      661 nylgigllyl ilqhlicilf ahtckwiyla slfagivige mtlaggvtlh lenlpgwyqk
      721 ispmqwslsl llpllhqtdt mnkltnckpk qiqrqdiivq aaceppdgal alyeialnrl
      781 nvqgelwlgi giaivsvliv lvflcirytt pkrlrsapnk p
//
LOCUS       XP_034185352             625 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185352
VERSION     XP_034185352.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329461.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..625
                     /product="ATP-binding cassette sub-family G member 5"
                     /calculated_mol_wt=68482
     Region          24..620
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     Region          34..231
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            52..59
                     /site_type="other"
                     /note="Walker A/P-loop"
                     /db_xref="CDD:213179"
     Site            order(55..56,58..60,98,172..173,206)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:213179"
     Site            95..98
                     /site_type="other"
                     /note="Q-loop/lid"
                     /db_xref="CDD:213179"
     Site            148..157
                     /site_type="other"
                     /note="ABC transporter signature motif"
                     /db_xref="CDD:213179"
     Site            168..173
                     /site_type="other"
                     /note="Walker B"
                     /db_xref="CDD:213179"
     Site            176..179
                     /site_type="other"
                     /note="D-loop"
                     /db_xref="CDD:213179"
     Site            202..208
                     /site_type="other"
                     /note="H-loop/switch region"
                     /db_xref="CDD:213179"
     CDS             1..625
                     /gene="LOC117606681"
                     /coded_by="XM_034329461.2:206..2083"
                     /db_xref="GeneID:117606681"
ORIGIN      
        1 mipsdctldi snvfhstavv iegsclskse ptavlrdvsa rvhggevlai lgskgsgkra
       61 lldviagrae getrgrvtln nnlltpelfr rhggyvahrc hllpsltvrq tltyatwlan
      121 lsnrdarvrq tladlalsqv anrpvndltk peyrrlmlgv qlakdptlll ldeptwdtdp
      181 lntylivsml wsyatrrgsi vvltmetprs dvlpfvsrvt llclgavvys gptrsmldyf
      241 tyigfpcpel enplmyylcl stvdrrsrdr flesnqqisv lvekfkaega vymkeapqvp
      301 pnvkdaalgi mhkglgrgik pgcfstllal ylrgmaatfg fnksglghfa arllllpfsv
      361 almsilyshs tpvqsriflq tgglmfnvlt lfyvagiast allfpgyrar yyqekregly
      421 ggamfltaya llslplsfis tmitigiltp ileldlsswa yacgvlwasy vaaeqvtvav
      481 lmvvgkpitg aitvlymtlv slviasgair slkglpywla avstalpary aslalnqlai
      541 dvpeslnlhy nesfacpgip elcrypdgrs ylierftreg enisevlnvd lnllislafa
      601 vglsilnsvl yllplparvk akfre
//
LOCUS       XP_034185353             619 aa            linear   INV 03-SEP-2025
DEFINITION  kelch like ECH associated protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185353
VERSION     XP_034185353.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329462.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..619
                     /product="kelch like ECH associated protein 1"
                     /calculated_mol_wt=69668
     Region          59..580
                     /region_name="PHA03098"
                     /note="kelch-like protein; Provisional"
                     /db_xref="CDD:222983"
     Region          350..397
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          401..444
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          448..492
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          495..537
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          542..585
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     CDS             1..619
                     /gene="Keap1"
                     /coded_by="XM_034329462.2:1..1860"
                     /db_xref="GeneID:117606682"
ORIGIN      
        1 mdeedcfikk eapgsfksen vasnssfmtd dkgleftekh gdlgdmtfrm anyikdvmkm
       61 mfimrshhml tdvvlevgse lfyahkvila aaspyfkamf tgglkesemt rvklqgvspt
      121 smarllyfmy tgqvrvteit vcsllsaatm fqvsnvidac cvflerqldp tnaigianfa
      181 eqhgcqslyq kanqfivqhf sqicqeeefl qlsaiqlitl vrkdelnvqe erevynavlk
      241 wvkyneearg pkmehilhav rcqyltpnfl reqmkncdvl kkvpacreyl aqifkdltlh
      301 kkpivkertp ntrrviyiag gflkhsldvl egynvdektw tqhaklivpr sglggaflkg
      361 mfyavggrnn spektydsdw vdrynpvmdq wrtcspmsmp rhrvgvavmd gllyavggsa
      421 gaeyhnsvec ydpehdawsn vkpmhikrlg vgvavvnrll yaiggfdgvd rlnsvecyhp
      481 endewtmvss mkcsrsgagv anlgqyiyvv ggydgtrqln sverydtekd twefvssvti
      541 arsalsvtvl dgklyamggy dgehflsive iydpakdtwe qgvpmtsgrs ghasavsyhq
      601 cpihcdhldh nislekpps
//
LOCUS       XP_034185354             542 aa            linear   INV 03-SEP-2025
DEFINITION  succinyl-CoA:3-ketoacid CoA transferase [Osmia lignaria lignaria].
ACCESSION   XP_034185354
VERSION     XP_034185354.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="succinyl-CoA:3-ketoacid CoA transferase"
                     /calculated_mol_wt=58702
     Region          61..298
                     /region_name="AtoD"
                     /note="Acyl CoA:acetate/3-ketoacid CoA transferase, alpha
                     subunit [Lipid transport and metabolism]; COG1788"
                     /db_xref="CDD:441394"
     Region          319..526
                     /region_name="SugarP_isomerase"
                     /note="Sugar Phosphate Isomerase family; includes type A
                     ribose 5-phosphate isomerase (RPI_A),
                     glucosamine-6-phosphate (GlcN6P) deaminase, and
                     6-phosphogluconolactonase (6PGL). RPI catalyzes the
                     reversible conversion of ribose-5-phosphate to
                     ribulose...; cl00339"
                     /db_xref="CDD:469729"
     CDS             1..542
                     /gene="SCOT"
                     /coded_by="XM_034329463.2:202..1830"
                     /db_xref="GeneID:117606683"
ORIGIN      
        1 mifainragc lllrvstgsr cnyknfklsr icsvnfsikt dipevkeept vpppppvgeg
       61 kvfrnveevi sdmpngakll vggfglcgip enliaailkk gvkdltvvsn nagvedfglg
      121 lllkqqrikr missyvgenp efeklylsgk leveltpqgt laervragga gipafytpta
      181 fgtivqegnv ivkydekgna eisgeprnva qfngrnyvme taitgdyalv kawkadkagn
      241 lmfrktarnf npvmckaakv aiaeveeive igeidpdhvh lpgiyvdriv kgtkyekrve
      301 krytkqnmkp kkvtpagkar eriirraale fkngmyanlg igipmlacny ipknvkvilq
      361 sengilglgp fpdenevdpd linagketvt vlpgasffss desfamirgg hvnltmlgam
      421 evsengdlan wmipgtmvkg mggamdlvsa astkvvvtme hkarngspki lkqctlpvtg
      481 qqcvdliitd mavfeviqge glklieiapn idisevvsst gcefsvaddl kemgqveltd
      541 cq
//
LOCUS       XP_034185356             365 aa            linear   INV 03-SEP-2025
DEFINITION  glucoside xylosyltransferase 1 shams [Osmia lignaria lignaria].
ACCESSION   XP_034185356
VERSION     XP_034185356.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329465.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="glucoside xylosyltransferase 1 shams"
                     /calculated_mol_wt=42267
     Region          57..350
                     /region_name="Glyco_tranf_GTA_type"
                     /note="Glycosyltransferase family A (GT-A) includes
                     diverse families of glycosyl transferases with a common
                     GT-A type structural fold; cl11394"
                     /db_xref="CDD:472172"
     Site            order(62,64,91,161,163)
                     /site_type="active"
                     /db_xref="CDD:132997"
     CDS             1..365
                     /gene="shams"
                     /coded_by="XM_034329465.2:418..1515"
                     /db_xref="GeneID:117606684"
ORIGIN      
        1 mksvyklffl clifvaliil lnltnfsnki ynaqtkeaai lpipakkilp ppitgevtic
       61 vvvcgdrlde sltmlksalv ftrrplqfii laetniipaf nekltewkli snktfdfvvk
      121 pitfpegsda amwkklfkpc aaqrlflptv lndtdavlyv dtdtlflapp etiwdefkkm
      181 nssqlaalsp ehedpntgwy nrfakhpyyg klgvnsgvml mnltrmrefr wieyvipihk
      241 eyklkitwgd qdiiniifhy hpeklyvysc rynyrpdhcm ympvctkaek egalvlhgsr
      301 gtfhsqkqpp fraiyqamee yqlntdpydy llmpmrsyla lehksncgkv wkvfiiqpel
      361 hlgqq
//
LOCUS       XP_034185357             344 aa            linear   INV 03-SEP-2025
DEFINITION  PDZ domain-containing protein GIPC-like protein kermit [Osmia
            lignaria lignaria].
ACCESSION   XP_034185357
VERSION     XP_034185357.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329466.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185357.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..344
                     /product="PDZ domain-containing protein GIPC-like protein
                     kermit"
                     /calculated_mol_wt=37822
     Region          137..225
                     /region_name="PDZ_GIPC"
                     /note="PDZ domain of GIPC family proteins; cd06707"
                     /db_xref="CDD:467191"
     Site            order(151..157,167,170,200,204,207..208)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467191"
     Region          274..335
                     /region_name="GH2_GIPC"
                     /note="GIPC-homology 2 (GH2) domain found in the GIPC
                     family; cd21180"
                     /db_xref="CDD:409666"
     Site            order(282,285..290,321)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:409666"
     Site            order(283..287,289,291..292,320..322,324,326)
                     /site_type="other"
                     /note="myosin VI binding site [polypeptide binding]"
                     /db_xref="CDD:409666"
     CDS             1..344
                     /gene="kermit"
                     /coded_by="XM_034329466.2:531..1565"
                     /db_xref="GeneID:117606685"
ORIGIN      
        1 mplfkaraap rsptsdkems nggshygncq qngnkneqee sdnngtydqk ngaqeqrrss
       61 rvsrsplvfh cqlahgsptg lisgfsnvre lyqkiaecyd lpageilfct lnthkldmte
      121 llggqigvgd fifahkkgrp keielvktdd slgltitdng agysfikrik egsvidrikv
      181 ieigdhleki datslvgkrh yevarmlkei pkgttftlrl veplkngfag igprgelkkg
      241 kksgygsgke tlrfkadgsa qiiekvddta sigiqkinvi lesfmgiydt elatqiwela
      301 egkdnsmefa eaidnsdlee fgftddfvie lwgavtdara grhr
//
LOCUS       XP_076545933            1469 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase-like isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545933
VERSION     XP_076545933.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689818.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1469
                     /product="activated Cdc42 kinase-like isoform X1"
                     /calculated_mol_wt=165550
     Region          12..72
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          126..386
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266,287..291,300,334)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(248,252,287..291,300,334)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(265..275,277..284,287..293)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          391..439
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     CDS             1..1469
                     /gene="Ack-like"
                     /coded_by="XM_076689818.1:436..4845"
                     /db_xref="GeneID:117608967"
ORIGIN      
        1 mdtqggmsrn tgpglyeflm eaelqqyypg irgdlkvqtt aqlkyvteed lnaigmskpe
       61 mrrlkkyfqk hfpqnylskf kkmllpkree ptmggltmlp eerqdrppir vpnkhmipad
      121 aiivnkelgt gefgvvqqgv wtndgeriqv aikclsrerm qnnpieflke aaimhaidhe
      181 hivrlygvvl dtnslmlvte laplrsllec lkepslrasf pvlslcdfav qiadgmqyle
      241 vkrlihrdla arnilvfskn kvkisdfgls ralgvgkdyy qtnfnvnlkl piawcapeci
      301 sylkftsasd vwaygvtlwe mfsygfqpwa altghqilea idepnfqrle qpeccpkdyf
      361 almqqcwqhe pskrpkfsel inllpdlkpe qvqavqdste tgqlvyrqgd vitvldkgss
      421 ntlwkgvlnn gktgffnpah tiaylgsnlp snkpgeftrg dgknafssqr rkirtdmiss
      481 pqgdlkhtgh vgldgayfgd igflggkyph lprqvvtpyk pqedvtdnss qalnqdarst
      541 emnrellrdn rnaqqdkhen lwsdassevc hmansskqsv slnvagnnds lgadheyhei
      601 sdeenqdspl rfdktlnfdf gpsllaemdq mfrslgsspp ppppvhplst ehessnvrne
      661 lreiqakqsn kkkqatvspi nvkpisaadq ktlysaiama qeltarsmtd lehppesprt
      721 paspsrrrkf sfklphqhsp kpdrrhfsee aasipdiqwl csslqslsst vssieslgap
      781 stlklplwdk asaefcfaks relltkptaw tsymdiefda hildnaamrq nlvkstefle
      841 ngnkkgnync etmtdtsdkl nvlqqngkvp plsiensnfd fsrdqkrvst syvdryfeqs
      901 kyfdddgals cttektpyfg eekmdkydki nnleqpnrpg cysniqdgsa nkhsqqlsnk
      961 ygncdqqlnd vqceetsfdl mkdkpvnfef prskpvkfds srdkvanidl gtrpkisssf
     1021 sdsskmnipe fpkkidvskn rggkvafasr nsnqepksvi ygstdsiktn lkagesensr
     1081 anmeavrini qsfrkieqnq nghdgfpfvi snnplfiaes ekkespvyss seslrvnfqr
     1141 lrrksdaean nqvelnskll aekyqrevsg lesvknylds kkgqglnlgl gkltqnmiql
     1201 gkniaqnsrn iettaskslv tnmrnldisg qsqtplrsln iptqkpkhld lniplqcqsq
     1261 ismklnlmqk snppssptih qhileppkly qndntrkeva ktdvllekms ttttnmyrhr
     1321 tsslsenrkp pmknirrsfh ptndssedsd sishsetdir srlrykrrhk kvphnlrlnf
     1381 knkghflhpe sargfssiel cgkspppsts flnslsppfs srnnllswpe sapsssltft
     1441 snsdldsdts seypeftsdi ltfppspap
//
LOCUS       XP_076545934            1464 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase-like isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545934
VERSION     XP_076545934.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689819.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1464
                     /product="activated Cdc42 kinase-like isoform X2"
                     /calculated_mol_wt=165039
     Region          12..72
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          126..386
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266,287..291,300,334)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(248,252,287..291,300,334)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(265..275,277..284,287..293)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          391..439
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     CDS             1..1464
                     /gene="Ack-like"
                     /coded_by="XM_076689819.1:436..4830"
                     /db_xref="GeneID:117608967"
ORIGIN      
        1 mdtqggmsrn tgpglyeflm eaelqqyypg irgdlkvqtt aqlkyvteed lnaigmskpe
       61 mrrlkkyfqk hfpqnylskf kkmllpkree ptmggltmlp eerqdrppir vpnkhmipad
      121 aiivnkelgt gefgvvqqgv wtndgeriqv aikclsrerm qnnpieflke aaimhaidhe
      181 hivrlygvvl dtnslmlvte laplrsllec lkepslrasf pvlslcdfav qiadgmqyle
      241 vkrlihrdla arnilvfskn kvkisdfgls ralgvgkdyy qtnfnvnlkl piawcapeci
      301 sylkftsasd vwaygvtlwe mfsygfqpwa altghqilea idepnfqrle qpeccpkdyf
      361 almqqcwqhe pskrpkfsel inllpdlkpe qvqavqdste tgqlvyrqgd vitvldkgss
      421 ntlwkgvlnn gktgffnpah tiaylgsnlp snkpgeftrg dgknafssqr rkirtdmiss
      481 pqgdlkhtgh vgldgayfgd igflggkyph lprqvvtpyk pqedvtdnss qalnqdarst
      541 emnrellrdn rnaqqdkhen lwsdassevc hmansskqsv slnvagnnds lgadheyhei
      601 sdeenqdspl rfdktlnfdf gpsllaemdq mfrslgsspp ppppvhplst ehessnvrne
      661 lreiqakqsn kkkqatvkpi saadqktlys aiamaqelta rsmtdlehpp esprtpasps
      721 rrrkfsfklp hqhspkpdrr hfseeaasip diqwlcsslq slsstvssie slgapstlkl
      781 plwdkasaef cfaksrellt kptawtsymd iefdahildn aamrqnlvks teflengnkk
      841 gnyncetmtd tsdklnvlqq ngkvpplsie nsnfdfsrdq krvstsyvdr yfeqskyfdd
      901 dgalscttek tpyfgeekmd kydkinnleq pnrpgcysni qdgsankhsq qlsnkygncd
      961 qqlndvqcee tsfdlmkdkp vnfefprskp vkfdssrdkv anidlgtrpk isssfsdssk
     1021 mnipefpkki dvsknrggkv afasrnsnqe pksviygstd siktnlkage sensranmea
     1081 vriniqsfrk ieqnqnghdg fpfvisnnpl fiaesekkes pvyssseslr vnfqrlrrks
     1141 daeannqvel nskllaekyq revsglesvk nyldskkgqg lnlglgkltq nmiqlgknia
     1201 qnsrnietta skslvtnmrn ldisgqsqtp lrslniptqk pkhldlnipl qcqsqismkl
     1261 nlmqksnpps sptihqhile ppklyqndnt rkevaktdvl lekmsttttn myrhrtssls
     1321 enrkppmkni rrsfhptnds sedsdsishs etdirsrlry krrhkkvphn lrlnfknkgh
     1381 flhpesargf ssielcgksp ppstsflnsl sppfssrnnl lswpesapss sltftsnsdl
     1441 dsdtsseype ftsdiltfpp spap
//
LOCUS       XP_076545935            1463 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase-like isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545935
VERSION     XP_076545935.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689820.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1463
                     /product="activated Cdc42 kinase-like isoform X3"
                     /calculated_mol_wt=164960
     Region          6..66
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          120..380
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(122..124,130,145,147,193..196,200,242,246..247,249,
                     260,281..285,294,328)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(122..124,130,145,147,193..196,200,242,246..247,249,
                     260)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(242,246,281..285,294,328)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(259..269,271..278,281..287)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          385..433
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     CDS             1..1463
                     /gene="Ack-like"
                     /coded_by="XM_076689820.1:619..5010"
                     /db_xref="GeneID:117608967"
ORIGIN      
        1 msrntgpgly eflmeaelqq yypgirgdlk vqttaqlkyv teedlnaigm skpemrrlkk
       61 yfqkhfpqny lskfkkmllp kreeptmggl tmlpeerqdr ppirvpnkhm ipadaiivnk
      121 elgtgefgvv qqgvwtndge riqvaikcls rermqnnpie flkeaaimha idhehivrly
      181 gvvldtnslm lvtelaplrs lleclkepsl rasfpvlslc dfavqiadgm qylevkrlih
      241 rdlaarnilv fsknkvkisd fglsralgvg kdyyqtnfnv nlklpiawca pecisylkft
      301 sasdvwaygv tlwemfsygf qpwaaltghq ileaidepnf qrleqpeccp kdyfalmqqc
      361 wqhepskrpk fselinllpd lkpeqvqavq dstetgqlvy rqgdvitvld kgssntlwkg
      421 vlnngktgff npahtiaylg snlpsnkpge ftrgdgknaf ssqrrkirtd misspqgdlk
      481 htghvgldga yfgdigflgg kyphlprqvv tpykpqedvt dnssqalnqd arstemnrel
      541 lrdnrnaqqd khenlwsdas sevchmanss kqsvslnvag nndslgadhe yheisdeenq
      601 dsplrfdktl nfdfgpslla emdqmfrslg ssppppppvh plstehessn vrnelreiqa
      661 kqsnkkkqat vspinvkpis aadqktlysa iamaqeltar smtdlehppe sprtpaspsr
      721 rrkfsfklph qhspkpdrrh fseeaasipd iqwlcsslqs lsstvssies lgapstlklp
      781 lwdkasaefc faksrelltk ptawtsymdi efdahildna amrqnlvkst eflengnkkg
      841 nyncetmtdt sdklnvlqqn gkvpplsien snfdfsrdqk rvstsyvdry feqskyfddd
      901 galscttekt pyfgeekmdk ydkinnleqp nrpgcysniq dgsankhsqq lsnkygncdq
      961 qlndvqceet sfdlmkdkpv nfefprskpv kfdssrdkva nidlgtrpki sssfsdsskm
     1021 nipefpkkid vsknrggkva fasrnsnqep ksviygstds iktnlkages ensranmeav
     1081 riniqsfrki eqnqnghdgf pfvisnnplf iaesekkesp vyssseslrv nfqrlrrksd
     1141 aeannqveln skllaekyqr evsglesvkn yldskkgqgl nlglgkltqn miqlgkniaq
     1201 nsrniettas kslvtnmrnl disgqsqtpl rslniptqkp khldlniplq cqsqismkln
     1261 lmqksnppss ptihqhilep pklyqndntr kevaktdvll ekmsttttnm yrhrtsslse
     1321 nrkppmknir rsfhptndss edsdsishse tdirsrlryk rrhkkvphnl rlnfknkghf
     1381 lhpesargfs sielcgkspp pstsflnsls ppfssrnnll swpesapsss ltftsnsdld
     1441 sdtsseypef tsdiltfpps pap
//
LOCUS       XP_076545936            1443 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase-like isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076545936
VERSION     XP_076545936.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689821.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1443
                     /product="activated Cdc42 kinase-like isoform X4"
                     /calculated_mol_wt=162745
     Region          12..72
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          126..386
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266,287..291,300,334)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(248,252,287..291,300,334)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(265..275,277..284,287..293)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          391..439
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     CDS             1..1443
                     /gene="Ack-like"
                     /coded_by="XM_076689821.1:436..4767"
                     /db_xref="GeneID:117608967"
ORIGIN      
        1 mdtqggmsrn tgpglyeflm eaelqqyypg irgdlkvqtt aqlkyvteed lnaigmskpe
       61 mrrlkkyfqk hfpqnylskf kkmllpkree ptmggltmlp eerqdrppir vpnkhmipad
      121 aiivnkelgt gefgvvqqgv wtndgeriqv aikclsrerm qnnpieflke aaimhaidhe
      181 hivrlygvvl dtnslmlvte laplrsllec lkepslrasf pvlslcdfav qiadgmqyle
      241 vkrlihrdla arnilvfskn kvkisdfgls ralgvgkdyy qtnfnvnlkl piawcapeci
      301 sylkftsasd vwaygvtlwe mfsygfqpwa altghqilea idepnfqrle qpeccpkdyf
      361 almqqcwqhe pskrpkfsel inllpdlkpe qvqavqdste tgqlvyrqgd vitvldkgss
      421 ntlwkgvlnn gktgffnpah tiaylgsnlp snkpgeftrg dgknafssqr rkirtdmiss
      481 pqgdlkhtgh vgldgayfgd igflggkyph lprqvvtpyk pqedvtdnss qalnqdarst
      541 emnrellrdn rnaqqdkhag nndslgadhe yheisdeenq dsplrfdktl nfdfgpslla
      601 emdqmfrslg ssppppppvh plstehessn vrnelreiqa kqsnkkkqat vspinvkpis
      661 aadqktlysa iamaqeltar smtdlehppe sprtpaspsr rrkfsfklph qhspkpdrrh
      721 fseeaasipd iqwlcsslqs lsstvssies lgapstlklp lwdkasaefc faksrelltk
      781 ptawtsymdi efdahildna amrqnlvkst eflengnkkg nyncetmtdt sdklnvlqqn
      841 gkvpplsien snfdfsrdqk rvstsyvdry feqskyfddd galscttekt pyfgeekmdk
      901 ydkinnleqp nrpgcysniq dgsankhsqq lsnkygncdq qlndvqceet sfdlmkdkpv
      961 nfefprskpv kfdssrdkva nidlgtrpki sssfsdsskm nipefpkkid vsknrggkva
     1021 fasrnsnqep ksviygstds iktnlkages ensranmeav riniqsfrki eqnqnghdgf
     1081 pfvisnnplf iaesekkesp vyssseslrv nfqrlrrksd aeannqveln skllaekyqr
     1141 evsglesvkn yldskkgqgl nlglgkltqn miqlgkniaq nsrniettas kslvtnmrnl
     1201 disgqsqtpl rslniptqkp khldlniplq cqsqismkln lmqksnppss ptihqhilep
     1261 pklyqndntr kevaktdvll ekmsttttnm yrhrtsslse nrkppmknir rsfhptndss
     1321 edsdsishse tdirsrlryk rrhkkvphnl rlnfknkghf lhpesargfs sielcgkspp
     1381 pstsflnsls ppfssrnnll swpesapsss ltftsnsdld sdtsseypef tsdiltfpps
     1441 pap
//
LOCUS       XP_076545937            1387 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase-like isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076545937
VERSION     XP_076545937.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689822.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1387
                     /product="activated Cdc42 kinase-like isoform X5"
                     /calculated_mol_wt=156381
     Region          12..72
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          126..386
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266,287..291,300,334)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(248,252,287..291,300,334)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(265..275,277..284,287..293)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          391..439
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     CDS             1..1387
                     /gene="Ack-like"
                     /coded_by="XM_076689822.1:436..4599"
                     /db_xref="GeneID:117608967"
ORIGIN      
        1 mdtqggmsrn tgpglyeflm eaelqqyypg irgdlkvqtt aqlkyvteed lnaigmskpe
       61 mrrlkkyfqk hfpqnylskf kkmllpkree ptmggltmlp eerqdrppir vpnkhmipad
      121 aiivnkelgt gefgvvqqgv wtndgeriqv aikclsrerm qnnpieflke aaimhaidhe
      181 hivrlygvvl dtnslmlvte laplrsllec lkepslrasf pvlslcdfav qiadgmqyle
      241 vkrlihrdla arnilvfskn kvkisdfgls ralgvgkdyy qtnfnvnlkl piawcapeci
      301 sylkftsasd vwaygvtlwe mfsygfqpwa altghqilea idepnfqrle qpeccpkdyf
      361 almqqcwqhe pskrpkfsel inllpdlkpe qvqavqdste tgqlvyrqgd vitvldkgss
      421 ntlwkgvlnn gktgffnpah tiaylgsnlp snkpgeftrg dgknafssqr rkirtdmiss
      481 pqgdlkhtgh vgldgayfgd igflggkyph lprqvvtpyk pqedvtdnss qalnqdarst
      541 emnrellrdn rnaqqdkhen lwsdassevc hmansskqsv slnvagnnds lgadheyhei
      601 sdeenqdspl rfdktlnfdf gpsllaemdq mfrslgsspp ppppvhplst ehessnvrne
      661 lreiqakqsn kkkqatwlcs slqslsstvs sieslgapst lklplwdkas aefcfaksre
      721 lltkptawts ymdiefdahi ldnaamrqnl vkstefleng nkkgnyncet mtdtsdklnv
      781 lqqngkvppl siensnfdfs rdqkrvstsy vdryfeqsky fdddgalsct tektpyfgee
      841 kmdkydkinn leqpnrpgcy sniqdgsank hsqqlsnkyg ncdqqlndvq ceetsfdlmk
      901 dkpvnfefpr skpvkfdssr dkvanidlgt rpkisssfsd sskmnipefp kkidvsknrg
      961 gkvafasrns nqepksviyg stdsiktnlk agesensran meavriniqs frkieqnqng
     1021 hdgfpfvisn nplfiaesek kespvyssse slrvnfqrlr rksdaeannq velnskllae
     1081 kyqrevsgle svknyldskk gqglnlglgk ltqnmiqlgk niaqnsrnie ttaskslvtn
     1141 mrnldisgqs qtplrslnip tqkpkhldln iplqcqsqis mklnlmqksn ppssptihqh
     1201 ileppklyqn dntrkevakt dvllekmstt ttnmyrhrts slsenrkppm knirrsfhpt
     1261 ndssedsdsi shsetdirsr lrykrrhkkv phnlrlnfkn kghflhpesa rgfssielcg
     1321 kspppstsfl nslsppfssr nnllswpesa psssltftsn sdldsdtsse ypeftsdilt
     1381 fppspap
//
LOCUS       XP_076545938            1286 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase-like isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076545938
VERSION     XP_076545938.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689823.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1286
                     /product="activated Cdc42 kinase-like isoform X6"
                     /calculated_mol_wt=143323
     Region          12..72
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          126..386
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266,287..291,300,334)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(248,252,287..291,300,334)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(265..275,277..284,287..293)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          391..439
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     CDS             1..1286
                     /gene="Ack-like"
                     /coded_by="XM_076689823.1:436..4296"
                     /db_xref="GeneID:117608967"
ORIGIN      
        1 mdtqggmsrn tgpglyeflm eaelqqyypg irgdlkvqtt aqlkyvteed lnaigmskpe
       61 mrrlkkyfqk hfpqnylskf kkmllpkree ptmggltmlp eerqdrppir vpnkhmipad
      121 aiivnkelgt gefgvvqqgv wtndgeriqv aikclsrerm qnnpieflke aaimhaidhe
      181 hivrlygvvl dtnslmlvte laplrsllec lkepslrasf pvlslcdfav qiadgmqyle
      241 vkrlihrdla arnilvfskn kvkisdfgls ralgvgkdyy qtnfnvnlkl piawcapeci
      301 sylkftsasd vwaygvtlwe mfsygfqpwa altghqilea idepnfqrle qpeccpkdyf
      361 almqqcwqhe pskrpkfsel inllpdlkpe qvqavqdste tgqlvyrqgd vitvldkgss
      421 ntlwkgvlnn gktgffnpah tiaylgsnlp snkpgeftrg dgknafssqr rkirtdmiss
      481 pqgdlkhtgh vgldgayfgd igflggkyph lprqvvtpyk pqedvtdnss qalnqdarst
      541 emnrellrdn rnaqqdkhen lwsdassevc hmansskqsv slnvagnnds lgadheyhei
      601 sdeenqdspl rfdktlnfdf gpsllaemdq mfrslgsspp ppppvhplst ehessnvrne
      661 lreiqakqsn kkkqatvspi nvkpisaadq ktlysaiama qeltarsmtd lehppesprt
      721 paspsrrrkf sfklphqhsp kpdrrhfsee aasipdiqat lsdeakevyn slvetpsiea
      781 apdtnplrml rsgvpivrpr irgnkhatlg htvshqedls setfhfdayn sgsktlpkir
      841 apppphappp lpqtrhlerh hsaqeiennq nqsnvdenpi plpprdrskt lqpksslprh
      901 qrkhpliipg ggvtrtlakm avttppiedq vdgslqsass svassslqed yssetsvnsp
      961 eefeqridse laaldtlpad enklhrfsvi sedllefsdn lidcdtsdnl vdcdtseyrq
     1021 nsqesrsdaq stdsdrqqrk isveskcsfr ssaskciqes etgeasrnst ptgssrseds
     1081 vvsgkaepkl qtsnartpsv qrpsnytmqy dygeypeggs qsrtskqpae vaeskesstv
     1141 dsndsfhssg ltstydrlsn eklsahtsdl srqsdhvsce dllefacdgp narrtrgprn
     1201 gkqsdevrim mkvlhehstp esciaalnvt dwdvlaaikl erlqgllkke nnfvglqdck
     1261 vmlnqcggdv vkaaallrst ddtaav
//
LOCUS       XP_076545939            1281 aa            linear   INV 03-SEP-2025
DEFINITION  activated Cdc42 kinase-like isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076545939
VERSION     XP_076545939.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689824.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1281
                     /product="activated Cdc42 kinase-like isoform X7"
                     /calculated_mol_wt=142812
     Region          12..72
                     /region_name="SAM_TNK-like"
                     /note="SAM domain of TNK(ACK)-like non-receptor
                     tyrosine-protein kinases; cd09539"
                     /db_xref="CDD:188938"
     Region          126..386
                     /region_name="PTKc_Ack_like"
                     /note="Catalytic domain of the Protein Tyrosine Kinase,
                     Activated Cdc42-associated kinase; cd05040"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266,287..291,300,334)
                     /site_type="active"
                     /db_xref="CDD:270636"
     Site            order(128..130,136,151,153,199..202,206,248,252..253,255,
                     266)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270636"
     Site            order(248,252,287..291,300,334)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270636"
     Site            order(265..275,277..284,287..293)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270636"
     Region          391..439
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     CDS             1..1281
                     /gene="Ack-like"
                     /coded_by="XM_076689824.1:436..4281"
                     /db_xref="GeneID:117608967"
ORIGIN      
        1 mdtqggmsrn tgpglyeflm eaelqqyypg irgdlkvqtt aqlkyvteed lnaigmskpe
       61 mrrlkkyfqk hfpqnylskf kkmllpkree ptmggltmlp eerqdrppir vpnkhmipad
      121 aiivnkelgt gefgvvqqgv wtndgeriqv aikclsrerm qnnpieflke aaimhaidhe
      181 hivrlygvvl dtnslmlvte laplrsllec lkepslrasf pvlslcdfav qiadgmqyle
      241 vkrlihrdla arnilvfskn kvkisdfgls ralgvgkdyy qtnfnvnlkl piawcapeci
      301 sylkftsasd vwaygvtlwe mfsygfqpwa altghqilea idepnfqrle qpeccpkdyf
      361 almqqcwqhe pskrpkfsel inllpdlkpe qvqavqdste tgqlvyrqgd vitvldkgss
      421 ntlwkgvlnn gktgffnpah tiaylgsnlp snkpgeftrg dgknafssqr rkirtdmiss
      481 pqgdlkhtgh vgldgayfgd igflggkyph lprqvvtpyk pqedvtdnss qalnqdarst
      541 emnrellrdn rnaqqdkhen lwsdassevc hmansskqsv slnvagnnds lgadheyhei
      601 sdeenqdspl rfdktlnfdf gpsllaemdq mfrslgsspp ppppvhplst ehessnvrne
      661 lreiqakqsn kkkqatvkpi saadqktlys aiamaqelta rsmtdlehpp esprtpasps
      721 rrrkfsfklp hqhspkpdrr hfseeaasip diqatlsdea kevynslvet psieaapdtn
      781 plrmlrsgvp ivrprirgnk hatlghtvsh qedlssetfh fdaynsgskt lpkirapppp
      841 happplpqtr hlerhhsaqe iennqnqsnv denpiplppr drsktlqpks slprhqrkhp
      901 liipgggvtr tlakmavttp piedqvdgsl qsasssvass slqedysset svnspeefeq
      961 ridselaald tlpadenklh rfsvisedll efsdnlidcd tsdnlvdcdt seyrqnsqes
     1021 rsdaqstdsd rqqrkisves kcsfrssask ciqesetgea srnstptgss rsedsvvsgk
     1081 aepklqtsna rtpsvqrpsn ytmqydygey peggsqsrts kqpaevaesk esstvdsnds
     1141 fhssgltsty drlsneklsa htsdlsrqsd hvscedllef acdgpnarrt rgprngkqsd
     1201 evrimmkvlh ehstpescia alnvtdwdvl aaiklerlqg llkkennfvg lqdckvmlnq
     1261 cggdvvkaaa llrstddtaa v
//
LOCUS       XP_034190653             350 aa            linear   INV 03-SEP-2025
DEFINITION  twinfilin actin binding protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190653
VERSION     XP_034190653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334762.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="twinfilin actin binding protein isoform X1"
                     /calculated_mol_wt=39381
     Region          4..143
                     /region_name="ADF_Twf-N_like"
                     /note="N-terminal ADF domain of twinfilin and related
                     proteins; cd11285"
                     /db_xref="CDD:200441"
     Site            5..6
                     /site_type="other"
                     /note="putative G-actin interface [polypeptide binding]"
                     /db_xref="CDD:200441"
     Region          177..311
                     /region_name="ADF_Twf-C_like"
                     /note="C-terminal ADF domain of twinfilin and related
                     proteins; cd11284"
                     /db_xref="CDD:200440"
     Site            order(177..181,211,258,260,263,267..268,270..272,274..275,
                     277,294,296,298)
                     /site_type="other"
                     /note="G-actin binding interface [polypeptide binding]"
                     /db_xref="CDD:200440"
     Site            order(268,299,302)
                     /site_type="other"
                     /note="putative F-actin interface [polypeptide binding]"
                     /db_xref="CDD:200440"
     CDS             1..350
                     /gene="twf"
                     /coded_by="XM_034334762.2:130..1182"
                     /db_xref="GeneID:117608965"
ORIGIN      
        1 mshqtgikan dalkklfakc rdgkirvlkv sieneelapa afskpankwq ddydkmikpl
       61 ivenqpayil yrldtkspds gydwlfisws pdtapvrqkm lyastkatlk qefgtasike
      121 elhgtvpedi tlegyhkykr ndaapapltt aeeelaelkk ttvttdysve trhqtlsgva
      181 fpvtdeakqa itdlgkgihe yvqlkidlee ekihlvtacd vsldklptkv psdsaryhly
      241 nfkhthegdy mecivfiysm pgyscsiker mlyssckapl ldliqslgvs itkkleiddg
      301 kelteeffqe elhpkislhq pkfakpkgpp grgakrltkv qdigtseqei
//
LOCUS       XP_034190654             348 aa            linear   INV 03-SEP-2025
DEFINITION  twinfilin actin binding protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190654
VERSION     XP_034190654.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334763.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..348
                     /product="twinfilin actin binding protein isoform X2"
                     /calculated_mol_wt=38838
     Region          4..143
                     /region_name="ADF_Twf-N_like"
                     /note="N-terminal ADF domain of twinfilin and related
                     proteins; cd11285"
                     /db_xref="CDD:200441"
     Site            5..6
                     /site_type="other"
                     /note="putative G-actin interface [polypeptide binding]"
                     /db_xref="CDD:200441"
     Region          177..303
                     /region_name="ADF_Twf-C_like"
                     /note="C-terminal ADF domain of twinfilin and related
                     proteins; cd11284"
                     /db_xref="CDD:200440"
     Site            order(177..181,211,258,260,263,267..268,270..272,274..275,
                     277,294,296,298)
                     /site_type="other"
                     /note="G-actin binding interface [polypeptide binding]"
                     /db_xref="CDD:200440"
     Site            order(268,299,302)
                     /site_type="other"
                     /note="putative F-actin interface [polypeptide binding]"
                     /db_xref="CDD:200440"
     CDS             1..348
                     /gene="twf"
                     /coded_by="XM_034334763.2:131..1177"
                     /db_xref="GeneID:117608965"
ORIGIN      
        1 mshqtgikan dalkklfakc rdgkirvlkv sieneelapa afskpankwq ddydkmikpl
       61 ivenqpayil yrldtkspds gydwlfisws pdtapvrqkm lyastkatlk qefgtasike
      121 elhgtvpedi tlegyhkykr ndaapapltt aeeelaelkk ttvttdysve trhqtlsgva
      181 fpvtdeakqa itdlgkgihe yvqlkidlee ekihlvtacd vsldklptkv psdsaryhly
      241 nfkhthegdy mecivfiysm pgyscsiker mlyssckapl ldliqslgvs itkklevnsg
      301 eeladmledp ptiketaait patpstpyap tqsipekksk qkrscvls
//
LOCUS       XP_034190655             304 aa            linear   INV 03-SEP-2025
DEFINITION  twinfilin actin binding protein isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190655
VERSION     XP_034190655.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334764.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="twinfilin actin binding protein isoform X3"
                     /calculated_mol_wt=34224
     Region          4..143
                     /region_name="ADF_Twf-N_like"
                     /note="N-terminal ADF domain of twinfilin and related
                     proteins; cd11285"
                     /db_xref="CDD:200441"
     Site            5..6
                     /site_type="other"
                     /note="putative G-actin interface [polypeptide binding]"
                     /db_xref="CDD:200441"
     Region          177..304
                     /region_name="ADF_Twf-C_like"
                     /note="C-terminal ADF domain of twinfilin and related
                     proteins; cd11284"
                     /db_xref="CDD:200440"
     Site            order(177..181,211,258,260,263,267..268,270..272,274..275,
                     277,294,297)
                     /site_type="other"
                     /note="G-actin binding interface [polypeptide binding]"
                     /db_xref="CDD:200440"
     Site            order(268,298,301)
                     /site_type="other"
                     /note="putative F-actin interface [polypeptide binding]"
                     /db_xref="CDD:200440"
     CDS             1..304
                     /gene="twf"
                     /coded_by="XM_034334764.2:130..1044"
                     /db_xref="GeneID:117608965"
ORIGIN      
        1 mshqtgikan dalkklfakc rdgkirvlkv sieneelapa afskpankwq ddydkmikpl
       61 ivenqpayil yrldtkspds gydwlfisws pdtapvrqkm lyastkatlk qefgtasike
      121 elhgtvpedi tlegyhkykr ndaapapltt aeeelaelkk ttvttdysve trhqtlsgva
      181 fpvtdeakqa itdlgkgihe yvqlkidlee ekihlvtacd vsldklptkv psdsaryhly
      241 nfkhthegdy mecivfiysm pgyscsiker mlyssckapl ldliqslgvs itkkmykkss
      301 eltv
//
LOCUS       XP_034190657             258 aa            linear   INV 03-SEP-2025
DEFINITION  RPII140-upstream gene protein [Osmia lignaria lignaria].
ACCESSION   XP_034190657
VERSION     XP_034190657.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334766.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..258
                     /product="RPII140-upstream gene protein"
                     /calculated_mol_wt=28050
     Region          64..182
                     /region_name="Tim17"
                     /note="Tim17/Tim22/Tim23/Pmp24 family; pfam02466"
                     /db_xref="CDD:460567"
     Region          <135..258
                     /region_name="TIM22"
                     /note="Mitochondrial import inner membrane translocase,
                     subunit TIM22 [Posttranslational modification, protein
                     turnover, chaperones]; cl02381"
                     /db_xref="CDD:470560"
     CDS             1..258
                     /gene="140up"
                     /coded_by="XM_034334766.2:156..932"
                     /db_xref="GeneID:117608966"
ORIGIN      
        1 mlrlainrgv apvallpffg gpsqndavpe dklaeitpmk vgmdkvkdff idengsttke
       61 lqsvinttaa gmvvgfitgg varsrfvten fiadnqatif knhyeakkll hykvavaick
      121 gglpfslklg tfcflfsgta aalqvykgkf dpwnhvigga iagavyktnm glkgavagcv
      181 lgtilgaisg svasiilyit gtemgdlyka snkwintrqk iileqrqfln eehkqfkqay
      241 ddhqnmrkam ygdekqeq
//
LOCUS       XP_034190658             258 aa            linear   INV 03-SEP-2025
DEFINITION  RPII140-upstream gene protein [Osmia lignaria lignaria].
ACCESSION   XP_034190658
VERSION     XP_034190658.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334767.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..258
                     /product="RPII140-upstream gene protein"
                     /calculated_mol_wt=28050
     Region          64..182
                     /region_name="Tim17"
                     /note="Tim17/Tim22/Tim23/Pmp24 family; pfam02466"
                     /db_xref="CDD:460567"
     Region          <135..258
                     /region_name="TIM22"
                     /note="Mitochondrial import inner membrane translocase,
                     subunit TIM22 [Posttranslational modification, protein
                     turnover, chaperones]; cl02381"
                     /db_xref="CDD:470560"
     CDS             1..258
                     /gene="140up"
                     /coded_by="XM_034334767.2:235..1011"
                     /db_xref="GeneID:117608966"
ORIGIN      
        1 mlrlainrgv apvallpffg gpsqndavpe dklaeitpmk vgmdkvkdff idengsttke
       61 lqsvinttaa gmvvgfitgg varsrfvten fiadnqatif knhyeakkll hykvavaick
      121 gglpfslklg tfcflfsgta aalqvykgkf dpwnhvigga iagavyktnm glkgavagcv
      181 lgtilgaisg svasiilyit gtemgdlyka snkwintrqk iileqrqfln eehkqfkqay
      241 ddhqnmrkam ygdekqeq
//
LOCUS       XP_034190687             624 aa            linear   INV 03-SEP-2025
DEFINITION  acetylcholinesterase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034190687
VERSION     XP_034190687.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334796.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="acetylcholinesterase 2"
                     /calculated_mol_wt=70214
     Region          33..572
                     /region_name="COesterase"
                     /note="Carboxylesterase family; pfam00135"
                     /db_xref="CDD:395084"
     Site            order(161..163,247..249,252,402,406..407,446,492,495)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(248,377,491)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..624
                     /gene="AChE-2"
                     /coded_by="XM_034334796.2:514..2388"
                     /db_xref="GeneID:117608983"
ORIGIN      
        1 mtdklpalll lilvctgsar anvtaqrsnv hndplvvett sglvrgfart vldkevhify
       61 gipfakppig plrfrkplpi epwhgvlnas alpnscyqer yeyfpgfpge emwnpntnis
      121 edclylniwv peknhrlrhk gsessnngek nglpmlvwiy gggfmsgtat ldvydadima
      181 avsnviiasm qyrvgafgfl ylnkhftnse eapgnmglwd qalalrwlrd naaafggdpd
      241 litifgesag gssvslhlis pvtrglvrrg ilqsgtlnap wsymtgekan evarvlvddc
      301 gcnstmlqen parvmacmrs vdaktisvqq wnsywgilgf psaptidgif lpkhpldllr
      361 eadfkdteil ignnknegty filydfidff ekdqasfler dkflniinti fknmsqierd
      421 aitfqytdwe evnngytyqk mvadvvgdyf ficpsihfaq lfadrgmkvy yyfftqrtst
      481 nlwgewmgvm hgdeveyvfg hpmntslmys dkernlsyrm iqyfskfays gkptneesew
      541 ppysrdqpqy fifdaertgl gkgprttsca fwneflprlq gvpdpiqeac nsaiassvsa
      601 gaenlrntla imvlssilaf srvi
//
LOCUS       XP_034185638             494 aa            linear   INV 03-SEP-2025
DEFINITION  muscle LIM protein Mlp84B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185638
VERSION     XP_034185638.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329747.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="muscle LIM protein Mlp84B isoform X1"
                     /calculated_mol_wt=53412
     Region          10..63
                     /region_name="LIM1_MLP84B_like"
                     /note="The LIM domain of Mlp84B and Mlp60A; cd09404"
                     /db_xref="CDD:188788"
     Site            order(11,14,32,35,38,41,59,62)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          117..170
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(117,120,138,141,144,147,149,166,169)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          219..272
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(219,222,240,243,246,249,251,268,271)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          322..375
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(322,325,343,346,349,352,354,371,374)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          420..473
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(420,423,441,444,447,450,452,469,472)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..494
                     /gene="LOC117606813"
                     /coded_by="XM_034329747.2:171..1655"
                     /db_xref="GeneID:117606813"
ORIGIN      
        1 mpfkpvehpk cpkcgksvya aeervagglk whkmcfkcgl cgklldstnc sehegelfck
       61 vchgrkfgpk gygfgggagc lsmdqgehlk sseelsrgsn avlesraiak apegegcprc
      121 ggyvyaaeqm largrayhks cykckvcqrs ldstlhcdgp dreiycracy pkkfgprgig
      181 hagvmwiglq cdiedegeaa prttvidtai ikappgkgcp rcggvvfaae qvlakgrewh
      241 rkcykchdct ktldsiiacd gpdkdvyckt cygkkwgphg ygfacgsgfl qtdglteeei
      301 sasrpfynpd ttsikapagq gcprcggmvf aaeqqlakgt mwhkkcfnca echrpldsml
      361 acdgpdkeih crscysklfg pkgfgfghap tlvstngdha psyidskpqv gqkrndghgc
      421 arcgypvyaa eqmisknrvw hkrcfscaec hrsldstnln dgpdgdiycr gcygrnfgpk
      481 gvgfgmgagt ltma
//
LOCUS       XP_034185639             493 aa            linear   INV 03-SEP-2025
DEFINITION  muscle LIM protein Mlp84B isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185639
VERSION     XP_034185639.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329748.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="muscle LIM protein Mlp84B isoform X2"
                     /calculated_mol_wt=53166
     Region          10..63
                     /region_name="LIM1_MLP84B_like"
                     /note="The LIM domain of Mlp84B and Mlp60A; cd09404"
                     /db_xref="CDD:188788"
     Site            order(11,14,32,35,38,41,59,62)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          117..170
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(117,120,138,141,144,147,149,166,169)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          218..271
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(218,221,239,242,245,248,250,267,270)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          321..374
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(321,324,342,345,348,351,353,370,373)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          419..472
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(419,422,440,443,446,449,451,468,471)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..493
                     /gene="LOC117606813"
                     /coded_by="XM_034329748.2:171..1652"
                     /db_xref="GeneID:117606813"
ORIGIN      
        1 mpfkpvehpk cpkcgksvya aeervagglk whkmcfkcgl cgklldstnc sehegelfck
       61 vchgrkfgpk gygfgggagc lsmdqgehlk sseelsrgsn avlesraiak apegegcprc
      121 ggyvyaaeqm largrqwhre cfkcancnkr ldsvnccegp dkdiyckvcy gkrfgpkgyg
      181 ygqgggalqs dcyangeaap rttvidtaii kappgkgcpr cggvvfaaeq vlakgrewhr
      241 kcykchdctk tldsiiacdg pdkdvycktc ygkkwgphgy gfacgsgflq tdglteeeis
      301 asrpfynpdt tsikapagqg cprcggmvfa aeqqlakgtm whkkcfncae chrpldsmla
      361 cdgpdkeihc rscysklfgp kgfgfghapt lvstngdhap syidskpqvg qkrndghgca
      421 rcgypvyaae qmisknrvwh krcfscaech rsldstnlnd gpdgdiycrg cygrnfgpkg
      481 vgfgmgagtl tma
//
LOCUS       XP_034185640             488 aa            linear   INV 03-SEP-2025
DEFINITION  muscle LIM protein Mlp84B isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185640
VERSION     XP_034185640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329749.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..488
                     /product="muscle LIM protein Mlp84B isoform X3"
                     /calculated_mol_wt=53979
     Region          111..164
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(111,114,132,135,138,141,143,160,163)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          213..266
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(213,216,234,237,240,243,245,262,265)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          316..369
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(316,319,337,340,343,346,348,365,368)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          414..467
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(414,417,435,438,441,444,446,463,466)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..488
                     /gene="LOC117606813"
                     /coded_by="XM_034329749.2:1725..3191"
                     /db_xref="GeneID:117606813"
ORIGIN      
        1 mdeparpsvn lhpvspkpwt pfvkkestsn rkkkrkeyym ytlnyprqpr rvranrrpie
       61 qymlslyhep artprdkgny rvtkskwels rgsnavlesr aiakapegeg cprcggyvya
      121 aeqmlargra yhkscykckv cqrsldstlh cdgpdreiyc racypkkfgp rgighagvmw
      181 iglqcdiede geaaprttvi dtaiikappg kgcprcggvv faaeqvlakg rewhrkcykc
      241 hdctktldsi iacdgpdkdv ycktcygkkw gphgygfacg sgflqtdglt eeeisasrpf
      301 ynpdttsika pagqgcprcg gmvfaaeqql akgtmwhkkc fncaechrpl dsmlacdgpd
      361 keihcrscys klfgpkgfgf ghaptlvstn gdhapsyids kpqvgqkrnd ghgcarcgyp
      421 vyaaeqmisk nrvwhkrcfs caechrslds tnlndgpdgd iycrgcygrn fgpkgvgfgm
      481 gagtltma
//
LOCUS       XP_034185642             487 aa            linear   INV 03-SEP-2025
DEFINITION  muscle LIM protein Mlp84B isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185642
VERSION     XP_034185642.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329751.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..487
                     /product="muscle LIM protein Mlp84B isoform X4"
                     /calculated_mol_wt=53732
     Region          111..164
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(111,114,132,135,138,141,143,160,163)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          212..265
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(212,215,233,236,239,242,244,261,264)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          315..368
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(315,318,336,339,342,345,347,364,367)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     Region          413..466
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(413,416,434,437,440,443,445,462,465)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..487
                     /gene="LOC117606813"
                     /coded_by="XM_034329751.2:1725..3188"
                     /db_xref="GeneID:117606813"
ORIGIN      
        1 mdeparpsvn lhpvspkpwt pfvkkestsn rkkkrkeyym ytlnyprqpr rvranrrpie
       61 qymlslyhep artprdkgny rvtkskwels rgsnavlesr aiakapegeg cprcggyvya
      121 aeqmlargrq whrecfkcan cnkrldsvnc cegpdkdiyc kvcygkrfgp kgygygqggg
      181 alqsdcyang eaaprttvid taiikappgk gcprcggvvf aaeqvlakgr ewhrkcykch
      241 dctktldsii acdgpdkdvy cktcygkkwg phgygfacgs gflqtdglte eeisasrpfy
      301 npdttsikap agqgcprcgg mvfaaeqqla kgtmwhkkcf ncaechrpld smlacdgpdk
      361 eihcrscysk lfgpkgfgfg haptlvstng dhapsyidsk pqvgqkrndg hgcarcgypv
      421 yaaeqmiskn rvwhkrcfsc aechrsldst nlndgpdgdi ycrgcygrnf gpkgvgfgmg
      481 agtltma
//
LOCUS       XP_034185643             221 aa            linear   INV 03-SEP-2025
DEFINITION  muscle LIM protein Mlp84B isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034185643
VERSION     XP_034185643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329752.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="muscle LIM protein Mlp84B isoform X5"
                     /calculated_mol_wt=24132
     Region          10..63
                     /region_name="LIM1_MLP84B_like"
                     /note="The LIM domain of Mlp84B and Mlp60A; cd09404"
                     /db_xref="CDD:188788"
     Site            order(11,14,32,35,38,41,59,62)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          117..170
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(117,120,138,141,144,147,149,166,169)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..221
                     /gene="LOC117606813"
                     /coded_by="XM_034329752.2:171..836"
                     /db_xref="GeneID:117606813"
ORIGIN      
        1 mpfkpvehpk cpkcgksvya aeervagglk whkmcfkcgl cgklldstnc sehegelfck
       61 vchgrkfgpk gygfgggagc lsmdqgehlk sseelsrgsn avlesraiak apegegcprc
      121 ggyvyaaeqm largrqwhre cfkcancnkr ldsvnccegp dkdiyckvcy gkrfgpkgyg
      181 ygqgggalqs dcyangllse eirsarywpc wgyvdraavr y
//
LOCUS       XP_034185644             175 aa            linear   INV 03-SEP-2025
DEFINITION  muscle LIM protein 1 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034185644
VERSION     XP_034185644.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329753.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="muscle LIM protein 1 isoform X6"
                     /calculated_mol_wt=19020
     Region          10..63
                     /region_name="LIM1_MLP84B_like"
                     /note="The LIM domain of Mlp84B and Mlp60A; cd09404"
                     /db_xref="CDD:188788"
     Site            order(11,14,32,35,38,41,59,62)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          117..168
                     /region_name="LIM_CRP_like"
                     /note="The LIM domains of Cysteine Rich Protein (CRP)
                     family; cd09326"
                     /db_xref="CDD:188712"
     Site            order(117,120,138,141,144,147,149,166)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..175
                     /gene="LOC117606813"
                     /coded_by="XM_034329753.2:171..698"
                     /db_xref="GeneID:117606813"
ORIGIN      
        1 mpfkpvehpk cpkcgksvya aeervagglk whkmcfkcgl cgklldstnc sehegelfck
       61 vchgrkfgpk gygfgggagc lsmdqgehlk sseelsrgsn avlesraiak apegegcprc
      121 ggyvyaaeqm largrayhks cykckvcqrs ldstlhcdgp dreiycrvrr lpvpp
//
LOCUS       XP_034185645             172 aa            linear   INV 03-SEP-2025
DEFINITION  muscle LIM protein 1 isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034185645
VERSION     XP_034185645.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329754.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..172
                     /product="muscle LIM protein 1 isoform X7"
                     /calculated_mol_wt=18358
     Region          10..63
                     /region_name="LIM1_MLP84B_like"
                     /note="The LIM domain of Mlp84B and Mlp60A; cd09404"
                     /db_xref="CDD:188788"
     Site            order(11,14,32,35,38,41,59,62)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          117..>135
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     CDS             1..172
                     /gene="LOC117606813"
                     /coded_by="XM_034329754.2:171..689"
                     /db_xref="GeneID:117606813"
ORIGIN      
        1 mpfkpvehpk cpkcgksvya aeervagglk whkmcfkcgl cgklldstnc sehegelfck
       61 vchgrkfgpk gygfgggagc lsmdqgehlk sseelsrgsn avlesraiak apegegcprc
      121 ggyvyaaeqm largrlairr nsvrevlaml glcgsgcsai lrtrvrrlpv pp
//
LOCUS       XP_034185646             313 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606814 [Osmia lignaria lignaria].
ACCESSION   XP_034185646
VERSION     XP_034185646.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329755.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..313
                     /product="uncharacterized protein LOC117606814"
                     /calculated_mol_wt=33052
     Region          139..195
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..313
                     /gene="LOC117606814"
                     /coded_by="XM_034329755.2:455..1396"
                     /db_xref="GeneID:117606814"
ORIGIN      
        1 mdysylnqaa aaaaagfeas scalaasemq cgygdlsscs qmsqaayryt aaraagypgn
       61 agtastgsst plgahhtths hahahhgaha tpcsvmaars qevhrhaasi fpsainlqsg
      121 lgykaysghd glaekrkqrr irttftsaql kelerafqet hypdiytree iamkidltea
      181 rvqvwfqnrr akfrkqerla qqksnsqsgi gvdsgasspv agnvkaegrs prspatppgs
      241 snsvlssnev kpitnpnlvs vkhssdtsge gtspntrgsn sgggtwgscs prpfsalppy
      301 lldplplkta nly
//
LOCUS       XP_034185616             925 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase 2C [Osmia lignaria lignaria].
ACCESSION   XP_034185616
VERSION     XP_034185616.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329725.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="protein phosphatase 2C"
                     /calculated_mol_wt=102256
     Region          10..328
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(20,24..25,49..51,257,323)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..925
                     /gene="Pp2C1"
                     /coded_by="XM_034329725.2:295..3072"
                     /db_xref="GeneID:117606805"
ORIGIN      
        1 mplsigvnlr vtghcnqggr kymedmfsva fqstpddkdl eyaffgifdg hgggeaatfa
       61 kehlmnvivk qknfwsdrde dvlrairdgy vnthyamwre ldkwprtasg lpstagttas
      121 iafirkgkiy ighvgdsgii lgyqvdgdpq wraraltkdh kpesgpemvr iresggkvvs
      181 ksgvprvvwn rprighkgpv rrsthideip flavarslgd lwsynseldt fvvspepdvk
      241 vvaidveshr clifgtdglw nmltpqaava ivqaadrhne khliasqqtc ngqadnvqlw
      301 inpskslvdr alerwsskrl radntsvvtl mldppgpsrs evilsqkecg lphhsaptlt
      361 atpvsenpek ltpspvvrsv prpspiithn vdlkrpdflp gtedsreprs dnpllvnkvd
      421 enfdlthesk dlmhenvenn piessiqvse tisngivdnl etsiaefekd stevierpdr
      481 eqtekldkqe dgidevthen esseskdrdd tnvknvihqs afsserndid sstcvssneq
      541 nsdfitesna neneiksdvl nkprhkqstl fsgprteirn grrghtlnah srntivdsgs
      601 legktyvnsr fklrstkvsv tgntveesvr nsasktrssa vgnkrrhsai sqsvvntsde
      661 scvlqessvk rrtrsedrrn pvdendpanq stkdassnls wpntesnitr stngattvpv
      721 qdrlslntfq rnlgkkatpv kairrpslkg llkqlwaasr garewdqgks nrttsscter
      781 rinrtmsiig spdtvsqrwl rsdtiaaatp vktlrsrnve inghtisaql vhqygmvkqs
      841 rlslpsklkq ssnngylqss rvrssslssg ikqnnngtgn gacstinpts tgcntssgma
      901 krvkspynps arslttrsri krlgk
//
LOCUS       XP_034185617             498 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185617
VERSION     XP_034185617.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329726.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="beta-1,4-glucuronyltransferase 1"
                     /calculated_mol_wt=55891
     Region          167..491
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..498
                     /gene="LOC117606806"
                     /coded_by="XM_034329726.2:634..2130"
                     /db_xref="GeneID:117606806"
ORIGIN      
        1 msmfsilrrn walrvsvvln icvllyvcah fgssgpwiee pptnwaaplq sdtfgsvelv
       61 ngtqkgatss alpatkdasg kavhgvnsts lartrlpttk ssletvdnrg rnqtvnsslp
      121 sarptmslkd ailcneksmn prraqrgdyw vlynyvpmsm avrcwesvty tthadytfld
      181 nlepllerwr apisiamhap gtdfaatlda ikysrncgst lisqlvtfhv ffsskhvpkv
      241 vppsekvvsd tyncslgppw vnvtfskmyk nekkllypvn vgrniaresa atyyvcasdi
      301 elypspnlpa kflemirkrd qpslykpnpk vfvlsifevd eksqppnnkt qlsqmlkagt
      361 aipfhkklcs schnvprske wqeapetegl hvfhvgkrtg sfvhwepifi gtnheplyde
      421 rlswegksdk mpqgyalcvl dydflildna flihrpgiki fkrdphreml taktnslikk
      481 iivpelkily gtrkgcav
//
LOCUS       XP_034185618             498 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034185618
VERSION     XP_034185618.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329727.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="beta-1,4-glucuronyltransferase 1"
                     /calculated_mol_wt=55891
     Region          167..491
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..498
                     /gene="LOC117606806"
                     /coded_by="XM_034329727.2:702..2198"
                     /db_xref="GeneID:117606806"
ORIGIN      
        1 msmfsilrrn walrvsvvln icvllyvcah fgssgpwiee pptnwaaplq sdtfgsvelv
       61 ngtqkgatss alpatkdasg kavhgvnsts lartrlpttk ssletvdnrg rnqtvnsslp
      121 sarptmslkd ailcneksmn prraqrgdyw vlynyvpmsm avrcwesvty tthadytfld
      181 nlepllerwr apisiamhap gtdfaatlda ikysrncgst lisqlvtfhv ffsskhvpkv
      241 vppsekvvsd tyncslgppw vnvtfskmyk nekkllypvn vgrniaresa atyyvcasdi
      301 elypspnlpa kflemirkrd qpslykpnpk vfvlsifevd eksqppnnkt qlsqmlkagt
      361 aipfhkklcs schnvprske wqeapetegl hvfhvgkrtg sfvhwepifi gtnheplyde
      421 rlswegksdk mpqgyalcvl dydflildna flihrpgiki fkrdphreml taktnslikk
      481 iivpelkily gtrkgcav
//
LOCUS       XP_034190417             618 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 12 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190417
VERSION     XP_034190417.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334526.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190417.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..618
                     /product="monocarboxylate transporter 12 isoform X1"
                     /calculated_mol_wt=67050
     Region          71..605
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(82..83,86..87,90,125,176..177,179..181,184,203,
                     206..207,210,436,439..440,443..445,448,475,479,530..531,
                     535,539,555,558..559,562..563,566)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     Region          <259..>413
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..618
                     /gene="LOC117608850"
                     /coded_by="XM_034334526.2:1191..3047"
                     /db_xref="GeneID:117608850"
ORIGIN      
        1 mkippagfps lsfslenhkl vsrrtvlsfr tgsrknfage fdqlystvtl saimtmksvk
       61 tklvppdggw gwvvlssalv vnflipgtvk sfgvlfvefl hvfkasstaa swmpalcyfl
      121 ynslgplssi lstrysykvv tiiggafaac gmmssyfans vsylyvsygl mvgigagltf
      181 pptvyivtsy ferlrgfang lcisgsaigt ivlppflqyl ldcfgyrgav limgaltlnt
      241 lvcgllyhpv eehmisvpie kgidnealti desivdkkkq tetqisydda gsekekqckd
      301 etiiddskvd hcskdskedt senekdtssi kdkdsnikeg dalndqvvsq ressaseidp
      361 tcdklkseks dlnnqlkdtk kdekicessk kdfgelseks tsktslskve ekksrchffd
      421 lsvlrdpiyl vilisnstsa isntnfmill psyaisqgfd knssalllsv vsaldlvgri
      481 sgaslsdidf vpkyyyfvgg lgtsgialal lpmatsyaml sffcalfgls sgmyigittv
      541 iladmlgtek lsssygislf vngvlqlvgp picgvvfeav gsykpiflsf giililgtal
      601 wavvplinrn nkknlqiv
//
LOCUS       XP_034190416             618 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 12 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190416
VERSION     XP_034190416.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334525.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190416.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..618
                     /product="monocarboxylate transporter 12 isoform X1"
                     /calculated_mol_wt=67050
     Region          71..605
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(82..83,86..87,90,125,176..177,179..181,184,203,
                     206..207,210,436,439..440,443..445,448,475,479,530..531,
                     535,539,555,558..559,562..563,566)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     Region          <259..>413
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..618
                     /gene="LOC117608850"
                     /coded_by="XM_034334525.2:744..2600"
                     /db_xref="GeneID:117608850"
ORIGIN      
        1 mkippagfps lsfslenhkl vsrrtvlsfr tgsrknfage fdqlystvtl saimtmksvk
       61 tklvppdggw gwvvlssalv vnflipgtvk sfgvlfvefl hvfkasstaa swmpalcyfl
      121 ynslgplssi lstrysykvv tiiggafaac gmmssyfans vsylyvsygl mvgigagltf
      181 pptvyivtsy ferlrgfang lcisgsaigt ivlppflqyl ldcfgyrgav limgaltlnt
      241 lvcgllyhpv eehmisvpie kgidnealti desivdkkkq tetqisydda gsekekqckd
      301 etiiddskvd hcskdskedt senekdtssi kdkdsnikeg dalndqvvsq ressaseidp
      361 tcdklkseks dlnnqlkdtk kdekicessk kdfgelseks tsktslskve ekksrchffd
      421 lsvlrdpiyl vilisnstsa isntnfmill psyaisqgfd knssalllsv vsaldlvgri
      481 sgaslsdidf vpkyyyfvgg lgtsgialal lpmatsyaml sffcalfgls sgmyigittv
      541 iladmlgtek lsssygislf vngvlqlvgp picgvvfeav gsykpiflsf giililgtal
      601 wavvplinrn nkknlqiv
//
LOCUS       XP_034190418             565 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 12 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190418
VERSION     XP_034190418.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334527.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..565
                     /product="monocarboxylate transporter 12 isoform X2"
                     /calculated_mol_wt=61164
     Region          18..552
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(29..30,33..34,37,72,123..124,126..128,131,150,
                     153..154,157,383,386..387,390..392,395,422,426,477..478,
                     482,486,502,505..506,509..510,513)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     Region          <206..>360
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..565
                     /gene="LOC117608850"
                     /coded_by="XM_034334527.2:661..2358"
                     /db_xref="GeneID:117608850"
ORIGIN      
        1 mtmksvktkl vppdggwgwv vlssalvvnf lipgtvksfg vlfveflhvf kasstaaswm
       61 palcyflyns lgplssilst rysykvvtii ggafaacgmm ssyfansvsy lyvsyglmvg
      121 igagltfppt vyivtsyfer lrgfanglci sgsaigtivl ppflqylldc fgyrgavlim
      181 galtlntlvc gllyhpveeh misvpiekgi dnealtides ivdkkkqtet qisyddagse
      241 kekqckdeti iddskvdhcs kdskedtsen ekdtssikdk dsnikegdal ndqvvsqres
      301 saseidptcd klkseksdln nqlkdtkkde kicesskkdf gelsekstsk tslskveekk
      361 srchffdlsv lrdpiylvil isnstsaisn tnfmillpsy aisqgfdkns salllsvvsa
      421 ldlvgrisga slsdidfvpk yyyfvgglgt sgialallpm atsyamlsff calfglssgm
      481 yigittvila dmlgteklss sygislfvng vlqlvgppic gvvfeavgsy kpiflsfgii
      541 lilgtalwav vplinrnnkk nlqiv
//
LOCUS       XP_034190422             549 aa            linear   INV 03-SEP-2025
DEFINITION  regulator of G-protein signaling 7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190422
VERSION     XP_034190422.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334531.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..549
                     /product="regulator of G-protein signaling 7 isoform X1"
                     /calculated_mol_wt=64075
     Region          76..162
                     /region_name="DEP_RGS7-like"
                     /note="DEP (Dishevelled, Egl-10, and Pleckstrin) domain
                     found in RGS (regulator of G-protein signaling) proteins
                     of the subfamily R7. This subgroup contains RGS7, RGS6,
                     RGS9 and RGS11. They share a common domain architecture,
                     containing, beside the RGS domain; cd04450"
                     /db_xref="CDD:239897"
     Region          159..260
                     /region_name="RGS_DHEX"
                     /note="Regulator of G-protein signalling DHEX domain;
                     pfam18148"
                     /db_xref="CDD:375589"
     Region          298..357
                     /region_name="GGL"
                     /note="G protein gamma subunit-like motifs; smart00224"
                     /db_xref="CDD:128520"
     Site            order(298,301,305,308,312,317,319,322..323,326..327,330,
                     339..340,349)
                     /site_type="other"
                     /note="beta subunit binding site [polypeptide binding]"
                     /db_xref="CDD:238024"
     Region          370..490
                     /region_name="RGS_R7-like"
                     /note="Regulator of G protein signaling (RGS) domain found
                     in the R7 subfamily of proteins; cd08705"
                     /db_xref="CDD:188660"
     Site            order(383,414..418,459..461,464,471,475)
                     /site_type="other"
                     /note="G-beta-5 interaction site"
                     /db_xref="CDD:188660"
     Site            order(398..400,402..403,436..438,440..442,444..446,469,
                     472..473,475..477,481)
                     /site_type="other"
                     /note="G-alpha interaction site"
                     /db_xref="CDD:188660"
     CDS             1..549
                     /gene="RSG7"
                     /coded_by="XM_034334531.2:722..2371"
                     /db_xref="GeneID:117608852"
ORIGIN      
        1 mvtmnseksa crrkqnensd iggtterlrn rdtqinqynn qndeavdksp ssptyhitas
       61 cvedtpnflv yqkmedivek msdevtgvpv ktvknfmtkt psvftgtdli swmmktmvid
      121 dqealhvahl mashgyffpi ddhcltvknd ntfyrfqtpy fwpsncwepe ntdyavylck
      181 rtmqnktrle ladyeaenla klqkmfsrkw efifmqaeaq skvdkkrdkl erkvldsqer
      241 afwdvyrpmp gcvntteldi kkacrtykpi srsnkhlqlg lgstqisphs esyaafsvea
      301 lnkeiemlks rldrtnikvs kvsealiayf eqyveydpff iqpeltnpwi sdnsemweqe
      361 klakevstrr vkrwafslqe llqdpvgreq firfldkefs genlkfweav qelkalpqke
      421 vqtkvneiwn eylgsdascp invdsqsyev tkknlqkadr wcfdiaaahv yhlmksdsys
      481 rylrsemykd ylngskkkaf fslllirllt aallptlyvs lscfllsttn kftnflfhyp
      541 nvtilffvl
//
LOCUS       XP_034190423             549 aa            linear   INV 03-SEP-2025
DEFINITION  regulator of G-protein signaling 7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190423
VERSION     XP_034190423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..549
                     /product="regulator of G-protein signaling 7 isoform X1"
                     /calculated_mol_wt=64075
     Region          76..162
                     /region_name="DEP_RGS7-like"
                     /note="DEP (Dishevelled, Egl-10, and Pleckstrin) domain
                     found in RGS (regulator of G-protein signaling) proteins
                     of the subfamily R7. This subgroup contains RGS7, RGS6,
                     RGS9 and RGS11. They share a common domain architecture,
                     containing, beside the RGS domain; cd04450"
                     /db_xref="CDD:239897"
     Region          159..260
                     /region_name="RGS_DHEX"
                     /note="Regulator of G-protein signalling DHEX domain;
                     pfam18148"
                     /db_xref="CDD:375589"
     Region          298..357
                     /region_name="GGL"
                     /note="G protein gamma subunit-like motifs; smart00224"
                     /db_xref="CDD:128520"
     Site            order(298,301,305,308,312,317,319,322..323,326..327,330,
                     339..340,349)
                     /site_type="other"
                     /note="beta subunit binding site [polypeptide binding]"
                     /db_xref="CDD:238024"
     Region          370..490
                     /region_name="RGS_R7-like"
                     /note="Regulator of G protein signaling (RGS) domain found
                     in the R7 subfamily of proteins; cd08705"
                     /db_xref="CDD:188660"
     Site            order(383,414..418,459..461,464,471,475)
                     /site_type="other"
                     /note="G-beta-5 interaction site"
                     /db_xref="CDD:188660"
     Site            order(398..400,402..403,436..438,440..442,444..446,469,
                     472..473,475..477,481)
                     /site_type="other"
                     /note="G-alpha interaction site"
                     /db_xref="CDD:188660"
     CDS             1..549
                     /gene="RSG7"
                     /coded_by="XM_034334532.2:125..1774"
                     /db_xref="GeneID:117608852"
ORIGIN      
        1 mvtmnseksa crrkqnensd iggtterlrn rdtqinqynn qndeavdksp ssptyhitas
       61 cvedtpnflv yqkmedivek msdevtgvpv ktvknfmtkt psvftgtdli swmmktmvid
      121 dqealhvahl mashgyffpi ddhcltvknd ntfyrfqtpy fwpsncwepe ntdyavylck
      181 rtmqnktrle ladyeaenla klqkmfsrkw efifmqaeaq skvdkkrdkl erkvldsqer
      241 afwdvyrpmp gcvntteldi kkacrtykpi srsnkhlqlg lgstqisphs esyaafsvea
      301 lnkeiemlks rldrtnikvs kvsealiayf eqyveydpff iqpeltnpwi sdnsemweqe
      361 klakevstrr vkrwafslqe llqdpvgreq firfldkefs genlkfweav qelkalpqke
      421 vqtkvneiwn eylgsdascp invdsqsyev tkknlqkadr wcfdiaaahv yhlmksdsys
      481 rylrsemykd ylngskkkaf fslllirllt aallptlyvs lscfllsttn kftnflfhyp
      541 nvtilffvl
//
LOCUS       XP_034190424             548 aa            linear   INV 03-SEP-2025
DEFINITION  regulator of G-protein signaling 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190424
VERSION     XP_034190424.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334533.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..548
                     /product="regulator of G-protein signaling 7 isoform X2"
                     /calculated_mol_wt=63946
     Region          76..161
                     /region_name="DEP_RGS7-like"
                     /note="DEP (Dishevelled, Egl-10, and Pleckstrin) domain
                     found in RGS (regulator of G-protein signaling) proteins
                     of the subfamily R7. This subgroup contains RGS7, RGS6,
                     RGS9 and RGS11. They share a common domain architecture,
                     containing, beside the RGS domain; cd04450"
                     /db_xref="CDD:239897"
     Region          158..259
                     /region_name="RGS_DHEX"
                     /note="Regulator of G-protein signalling DHEX domain;
                     pfam18148"
                     /db_xref="CDD:375589"
     Region          297..356
                     /region_name="GGL"
                     /note="G protein gamma subunit-like motifs; smart00224"
                     /db_xref="CDD:128520"
     Site            order(297,300,304,307,311,316,318,321..322,325..326,329,
                     338..339,348)
                     /site_type="other"
                     /note="beta subunit binding site [polypeptide binding]"
                     /db_xref="CDD:238024"
     Region          369..489
                     /region_name="RGS_R7-like"
                     /note="Regulator of G protein signaling (RGS) domain found
                     in the R7 subfamily of proteins; cd08705"
                     /db_xref="CDD:188660"
     Site            order(382,413..417,458..460,463,470,474)
                     /site_type="other"
                     /note="G-beta-5 interaction site"
                     /db_xref="CDD:188660"
     Site            order(397..399,401..402,435..437,439..441,443..445,468,
                     471..472,474..476,480)
                     /site_type="other"
                     /note="G-alpha interaction site"
                     /db_xref="CDD:188660"
     CDS             1..548
                     /gene="RSG7"
                     /coded_by="XM_034334533.2:723..2369"
                     /db_xref="GeneID:117608852"
ORIGIN      
        1 mvtmnseksa crrkqnensd iggtterlrn rdtqinqynn qndeavdksp ssptyhitas
       61 cvedtpnflv yqkmedivek msdevtgvpv ktvknfmtkt psvftgtdli swmmktmvid
      121 dqalhvahlm ashgyffpid dhcltvkndn tfyrfqtpyf wpsncwepen tdyavylckr
      181 tmqnktrlel adyeaenlak lqkmfsrkwe fifmqaeaqs kvdkkrdkle rkvldsqera
      241 fwdvyrpmpg cvntteldik kacrtykpis rsnkhlqlgl gstqisphse syaafsveal
      301 nkeiemlksr ldrtnikvsk vsealiayfe qyveydpffi qpeltnpwis dnsemweqek
      361 lakevstrrv krwafslqel lqdpvgreqf irfldkefsg enlkfweavq elkalpqkev
      421 qtkvneiwne ylgsdascpi nvdsqsyevt kknlqkadrw cfdiaaahvy hlmksdsysr
      481 ylrsemykdy lngskkkaff slllirllta allptlyvsl scfllsttnk ftnflfhypn
      541 vtilffvl
//
LOCUS       XP_034190425             519 aa            linear   INV 03-SEP-2025
DEFINITION  regulator of G-protein signaling 7 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190425
VERSION     XP_034190425.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334534.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="regulator of G-protein signaling 7 isoform X3"
                     /calculated_mol_wt=60615
     Region          76..162
                     /region_name="DEP_RGS7-like"
                     /note="DEP (Dishevelled, Egl-10, and Pleckstrin) domain
                     found in RGS (regulator of G-protein signaling) proteins
                     of the subfamily R7. This subgroup contains RGS7, RGS6,
                     RGS9 and RGS11. They share a common domain architecture,
                     containing, beside the RGS domain; cd04450"
                     /db_xref="CDD:239897"
     Region          159..260
                     /region_name="RGS_DHEX"
                     /note="Regulator of G-protein signalling DHEX domain;
                     pfam18148"
                     /db_xref="CDD:375589"
     Region          298..357
                     /region_name="GGL"
                     /note="G protein gamma subunit-like motifs; smart00224"
                     /db_xref="CDD:128520"
     Site            order(298,301,305,308,312,317,319,322..323,326..327,330,
                     339..340,349)
                     /site_type="other"
                     /note="beta subunit binding site [polypeptide binding]"
                     /db_xref="CDD:238024"
     Region          370..490
                     /region_name="RGS_R7-like"
                     /note="Regulator of G protein signaling (RGS) domain found
                     in the R7 subfamily of proteins; cd08705"
                     /db_xref="CDD:188660"
     Site            order(383,414..418,459..461,464,471,475)
                     /site_type="other"
                     /note="G-beta-5 interaction site"
                     /db_xref="CDD:188660"
     Site            order(398..400,402..403,436..438,440..442,444..446,469,
                     472..473,475..477,481)
                     /site_type="other"
                     /note="G-alpha interaction site"
                     /db_xref="CDD:188660"
     CDS             1..519
                     /gene="RSG7"
                     /coded_by="XM_034334534.2:721..2280"
                     /db_xref="GeneID:117608852"
ORIGIN      
        1 mvtmnseksa crrkqnensd iggtterlrn rdtqinqynn qndeavdksp ssptyhitas
       61 cvedtpnflv yqkmedivek msdevtgvpv ktvknfmtkt psvftgtdli swmmktmvid
      121 dqealhvahl mashgyffpi ddhcltvknd ntfyrfqtpy fwpsncwepe ntdyavylck
      181 rtmqnktrle ladyeaenla klqkmfsrkw efifmqaeaq skvdkkrdkl erkvldsqer
      241 afwdvyrpmp gcvntteldi kkacrtykpi srsnkhlqlg lgstqisphs esyaafsvea
      301 lnkeiemlks rldrtnikvs kvsealiayf eqyveydpff iqpeltnpwi sdnsemweqe
      361 klakevstrr vkrwafslqe llqdpvgreq firfldkefs genlkfweav qelkalpqke
      421 vqtkvneiwn eylgsdascp invdsqsyev tkknlqkadr wcfdiaaahv yhlmksdsys
      481 rylrsemykd ylngskkkaf fslllirllt aallptlrr
//
LOCUS       XP_034190426             519 aa            linear   INV 03-SEP-2025
DEFINITION  regulator of G-protein signaling 7 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190426
VERSION     XP_034190426.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334535.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="regulator of G-protein signaling 7 isoform X4"
                     /calculated_mol_wt=60442
     Region          76..162
                     /region_name="DEP_RGS7-like"
                     /note="DEP (Dishevelled, Egl-10, and Pleckstrin) domain
                     found in RGS (regulator of G-protein signaling) proteins
                     of the subfamily R7. This subgroup contains RGS7, RGS6,
                     RGS9 and RGS11. They share a common domain architecture,
                     containing, beside the RGS domain; cd04450"
                     /db_xref="CDD:239897"
     Region          159..260
                     /region_name="RGS_DHEX"
                     /note="Regulator of G-protein signalling DHEX domain;
                     pfam18148"
                     /db_xref="CDD:375589"
     Region          298..357
                     /region_name="GGL"
                     /note="G protein gamma subunit-like motifs; smart00224"
                     /db_xref="CDD:128520"
     Site            order(298,301,305,308,312,317,319,322..323,326..327,330,
                     339..340,349)
                     /site_type="other"
                     /note="beta subunit binding site [polypeptide binding]"
                     /db_xref="CDD:238024"
     Region          370..490
                     /region_name="RGS_R7-like"
                     /note="Regulator of G protein signaling (RGS) domain found
                     in the R7 subfamily of proteins; cd08705"
                     /db_xref="CDD:188660"
     Site            order(383,414..418,459..461,464,471,475)
                     /site_type="other"
                     /note="G-beta-5 interaction site"
                     /db_xref="CDD:188660"
     Site            order(398..400,402..403,436..438,440..442,444..446,469,
                     472..473,475..477,481)
                     /site_type="other"
                     /note="G-alpha interaction site"
                     /db_xref="CDD:188660"
     CDS             1..519
                     /gene="RSG7"
                     /coded_by="XM_034334535.2:721..2280"
                     /db_xref="GeneID:117608852"
ORIGIN      
        1 mvtmnseksa crrkqnensd iggtterlrn rdtqinqynn qndeavdksp ssptyhitas
       61 cvedtpnflv yqkmedivek msdevtgvpv ktvknfmtkt psvftgtdli swmmktmvid
      121 dqealhvahl mashgyffpi ddhcltvknd ntfyrfqtpy fwpsncwepe ntdyavylck
      181 rtmqnktrle ladyeaenla klqkmfsrkw efifmqaeaq skvdkkrdkl erkvldsqer
      241 afwdvyrpmp gcvntteldi kkacrtykpi srsnkhlqlg lgstqisphs esyaafsvea
      301 lnkeiemlks rldrtnikvs kvsealiayf eqyveydpff iqpeltnpwi sdnsemweqe
      361 klakevstrr vkrwafslqe llqdpvgreq firfldkefs genlkfweav qelkalpqke
      421 vqtkvneiwn eylgsdascp invdsqsyev tkknlqkadr wcfdiaaahv yhlmksdsys
      481 rylrsemykd ylngskkkts vkgirsivsf sgrrdtats
//
LOCUS       XP_034190427             540 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 26 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190427
VERSION     XP_034190427.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334536.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="mediator complex subunit 26 isoform X1"
                     /calculated_mol_wt=60067
     Region          9..>153
                     /region_name="TFSII"
                     /note="transcription elongation factor S-II; TIGR01385"
                     /db_xref="CDD:273592"
     Region          30..81
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          87..>226
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..540
                     /gene="MED26"
                     /coded_by="XM_034334536.2:215..1837"
                     /db_xref="GeneID:117608853"
ORIGIN      
        1 mqryctelte rllksldkey nvidmgavvd vitalektai tkevleitrl gkyinelrrk
       61 tnndalakra kdlvrrwrdm vlpsahstpq ptpadtappa lngakphspa lrclkpqspa
      121 lrgvvvppqs pllrdttplr vlspvlsvhs dhshspnass nkqsitvtsn hrtssdvppt
      181 lgsssqhhpm eavprthssn krlrkddfkd qpsyqhhdsd siteseslvk kqrlngenis
      241 gnlnlqvpsp tfkerisdqf messtdpdds gpkkrgrkkg sksmkkppvl edrvkeklas
      301 isrnpklktt qelladlrar gtnssvnssa lpsqsvessg medilrsnne qvskflrcpq
      361 nnvshrntvs easpesrlkp tenchdvpsk ceesgvvyke ksvatfqksq seqckeltve
      421 eilaklppid pssidwsecd tettedqnst eypprqvtae dierlhsqcv eglngnfqpk
      481 lssltscnei qdtnegvsen ngvnnvysqn geefrewhqm larpsydgqi lhilpyviid
//
LOCUS       XP_034190428             530 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 26 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190428
VERSION     XP_034190428.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334537.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..530
                     /product="mediator complex subunit 26 isoform X2"
                     /calculated_mol_wt=58690
     Region          16..>143
                     /region_name="TFSII"
                     /note="transcription elongation factor S-II; TIGR01385"
                     /db_xref="CDD:273592"
     Region          20..71
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          77..>216
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..530
                     /gene="MED26"
                     /coded_by="XM_034334537.2:16..1608"
                     /db_xref="GeneID:117608853"
ORIGIN      
        1 mhglrlggtw evidmgavvd vitalektai tkevleitrl gkyinelrrk tnndalakra
       61 kdlvrrwrdm vlpsahstpq ptpadtappa lngakphspa lrclkpqspa lrgvvvppqs
      121 pllrdttplr vlspvlsvhs dhshspnass nkqsitvtsn hrtssdvppt lgsssqhhpm
      181 eavprthssn krlrkddfkd qpsyqhhdsd siteseslvk kqrlngenis gnlnlqvpsp
      241 tfkerisdqf messtdpdds gpkkrgrkkg sksmkkppvl edrvkeklas isrnpklktt
      301 qelladlrar gtnssvnssa lpsqsvessg medilrsnne qvskflrcpq nnvshrntvs
      361 easpesrlkp tenchdvpsk ceesgvvyke ksvatfqksq seqckeltve eilaklppid
      421 pssidwsecd tettedqnst eypprqvtae dierlhsqcv eglngnfqpk lssltscnei
      481 qdtnegvsen ngvnnvysqn geefrewhqm larpsydgqi lhilpyviid
//
LOCUS       XP_034190433             516 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 26 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190433
VERSION     XP_034190433.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334542.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="mediator complex subunit 26 isoform X3"
                     /calculated_mol_wt=57124
     Region          2..>129
                     /region_name="TFSII"
                     /note="transcription elongation factor S-II; TIGR01385"
                     /db_xref="CDD:273592"
     Region          6..57
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          63..>202
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..516
                     /gene="MED26"
                     /coded_by="XM_034334542.2:429..1979"
                     /db_xref="GeneID:117608853"
ORIGIN      
        1 mgavvdvita lektaitkev leitrlgkyi nelrrktnnd alakrakdlv rrwrdmvlps
       61 ahstpqptpa dtappalnga kphspalrcl kpqspalrgv vvppqspllr dttplrvlsp
      121 vlsvhsdhsh spnassnkqs itvtsnhrts sdvpptlgss sqhhpmeavp rthssnkrlr
      181 kddfkdqpsy qhhdsdsite seslvkkqrl ngenisgnln lqvpsptfke risdqfmess
      241 tdpddsgpkk rgrkkgsksm kkppvledrv keklasisrn pklkttqell adlrargtns
      301 svnssalpsq svessgmedi lrsnneqvsk flrcpqnnvs hrntvseasp esrlkptenc
      361 hdvpskcees gvvykeksva tfqksqseqc keltveeila klppidpssi dwsecdtett
      421 edqnsteypp rqvtaedier lhsqcvegln gnfqpklssl tscneiqdtn egvsenngvn
      481 nvysqngeef rewhqmlarp sydgqilhil pyviid
//
LOCUS       XP_034190430             516 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 26 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190430
VERSION     XP_034190430.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334539.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="mediator complex subunit 26 isoform X3"
                     /calculated_mol_wt=57124
     Region          2..>129
                     /region_name="TFSII"
                     /note="transcription elongation factor S-II; TIGR01385"
                     /db_xref="CDD:273592"
     Region          6..57
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          63..>202
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..516
                     /gene="MED26"
                     /coded_by="XM_034334539.2:212..1762"
                     /db_xref="GeneID:117608853"
ORIGIN      
        1 mgavvdvita lektaitkev leitrlgkyi nelrrktnnd alakrakdlv rrwrdmvlps
       61 ahstpqptpa dtappalnga kphspalrcl kpqspalrgv vvppqspllr dttplrvlsp
      121 vlsvhsdhsh spnassnkqs itvtsnhrts sdvpptlgss sqhhpmeavp rthssnkrlr
      181 kddfkdqpsy qhhdsdsite seslvkkqrl ngenisgnln lqvpsptfke risdqfmess
      241 tdpddsgpkk rgrkkgsksm kkppvledrv keklasisrn pklkttqell adlrargtns
      301 svnssalpsq svessgmedi lrsnneqvsk flrcpqnnvs hrntvseasp esrlkptenc
      361 hdvpskcees gvvykeksva tfqksqseqc keltveeila klppidpssi dwsecdtett
      421 edqnsteypp rqvtaedier lhsqcvegln gnfqpklssl tscneiqdtn egvsenngvn
      481 nvysqngeef rewhqmlarp sydgqilhil pyviid
//
LOCUS       XP_034190429             516 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 26 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190429
VERSION     XP_034190429.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334538.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="mediator complex subunit 26 isoform X3"
                     /calculated_mol_wt=57124
     Region          2..>129
                     /region_name="TFSII"
                     /note="transcription elongation factor S-II; TIGR01385"
                     /db_xref="CDD:273592"
     Region          6..57
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          63..>202
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..516
                     /gene="MED26"
                     /coded_by="XM_034334538.2:146..1696"
                     /db_xref="GeneID:117608853"
ORIGIN      
        1 mgavvdvita lektaitkev leitrlgkyi nelrrktnnd alakrakdlv rrwrdmvlps
       61 ahstpqptpa dtappalnga kphspalrcl kpqspalrgv vvppqspllr dttplrvlsp
      121 vlsvhsdhsh spnassnkqs itvtsnhrts sdvpptlgss sqhhpmeavp rthssnkrlr
      181 kddfkdqpsy qhhdsdsite seslvkkqrl ngenisgnln lqvpsptfke risdqfmess
      241 tdpddsgpkk rgrkkgsksm kkppvledrv keklasisrn pklkttqell adlrargtns
      301 svnssalpsq svessgmedi lrsnneqvsk flrcpqnnvs hrntvseasp esrlkptenc
      361 hdvpskcees gvvykeksva tfqksqseqc keltveeila klppidpssi dwsecdtett
      421 edqnsteypp rqvtaedier lhsqcvegln gnfqpklssl tscneiqdtn egvsenngvn
      481 nvysqngeef rewhqmlarp sydgqilhil pyviid
//
LOCUS       XP_034190432             516 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 26 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190432
VERSION     XP_034190432.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334541.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="mediator complex subunit 26 isoform X3"
                     /calculated_mol_wt=57124
     Region          2..>129
                     /region_name="TFSII"
                     /note="transcription elongation factor S-II; TIGR01385"
                     /db_xref="CDD:273592"
     Region          6..57
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          63..>202
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..516
                     /gene="MED26"
                     /coded_by="XM_034334541.2:171..1721"
                     /db_xref="GeneID:117608853"
ORIGIN      
        1 mgavvdvita lektaitkev leitrlgkyi nelrrktnnd alakrakdlv rrwrdmvlps
       61 ahstpqptpa dtappalnga kphspalrcl kpqspalrgv vvppqspllr dttplrvlsp
      121 vlsvhsdhsh spnassnkqs itvtsnhrts sdvpptlgss sqhhpmeavp rthssnkrlr
      181 kddfkdqpsy qhhdsdsite seslvkkqrl ngenisgnln lqvpsptfke risdqfmess
      241 tdpddsgpkk rgrkkgsksm kkppvledrv keklasisrn pklkttqell adlrargtns
      301 svnssalpsq svessgmedi lrsnneqvsk flrcpqnnvs hrntvseasp esrlkptenc
      361 hdvpskcees gvvykeksva tfqksqseqc keltveeila klppidpssi dwsecdtett
      421 edqnsteypp rqvtaedier lhsqcvegln gnfqpklssl tscneiqdtn egvsenngvn
      481 nvysqngeef rewhqmlarp sydgqilhil pyviid
//
LOCUS       XP_034190434             516 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 26 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190434
VERSION     XP_034190434.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334543.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="mediator complex subunit 26 isoform X3"
                     /calculated_mol_wt=57124
     Region          2..>129
                     /region_name="TFSII"
                     /note="transcription elongation factor S-II; TIGR01385"
                     /db_xref="CDD:273592"
     Region          6..57
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          63..>202
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..516
                     /gene="MED26"
                     /coded_by="XM_034334543.2:102..1652"
                     /db_xref="GeneID:117608853"
ORIGIN      
        1 mgavvdvita lektaitkev leitrlgkyi nelrrktnnd alakrakdlv rrwrdmvlps
       61 ahstpqptpa dtappalnga kphspalrcl kpqspalrgv vvppqspllr dttplrvlsp
      121 vlsvhsdhsh spnassnkqs itvtsnhrts sdvpptlgss sqhhpmeavp rthssnkrlr
      181 kddfkdqpsy qhhdsdsite seslvkkqrl ngenisgnln lqvpsptfke risdqfmess
      241 tdpddsgpkk rgrkkgsksm kkppvledrv keklasisrn pklkttqell adlrargtns
      301 svnssalpsq svessgmedi lrsnneqvsk flrcpqnnvs hrntvseasp esrlkptenc
      361 hdvpskcees gvvykeksva tfqksqseqc keltveeila klppidpssi dwsecdtett
      421 edqnsteypp rqvtaedier lhsqcvegln gnfqpklssl tscneiqdtn egvsenngvn
      481 nvysqngeef rewhqmlarp sydgqilhil pyviid
//
LOCUS       XP_034190490             199 aa            linear   INV 03-SEP-2025
DEFINITION  ras-like protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034190490
VERSION     XP_034190490.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334599.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="ras-like protein 2"
                     /calculated_mol_wt=22768
     Region          12..175
                     /region_name="M_R_Ras_like"
                     /note="R-Ras2/TC21, M-Ras/R-Ras3; cd04145"
                     /db_xref="CDD:133345"
     Site            20..27
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133345"
     Site            order(21..22,69..70)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:133345"
     Site            order(23..28,40..41,126..127,129,156..157)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133345"
     Site            order(27..28,42,44,50..51,64..65,67,69..70,158)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:133345"
     Site            order(43..44,48..51)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:133345"
     Site            43..50
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133345"
     Site            45
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133345"
     Site            67..70
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133345"
     Site            69..87
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133345"
     Site            126..129
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133345"
     Site            156..158
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133345"
     CDS             1..199
                     /gene="Ras64B"
                     /coded_by="XM_034334599.2:321..920"
                     /db_xref="GeneID:117608873"
ORIGIN      
        1 mskpgdkqsy aqtyklvvvg gggvgksait iqfiqsyfvt dydptiedsy tkqcviddip
       61 akldildtag qeefsamreq ymrsgegfll vfsvtdhssf deilkfhrqi lrvkdrdefp
      121 mlmvgnkadl dyqrivsvee aqnmarqlki pyiecsaklr mnvdqafhel vrivrkfqls
      181 erppldlnhk knkkkccml
//
LOCUS       XP_034185349            1161 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin-G-associated kinase [Osmia lignaria lignaria].
ACCESSION   XP_034185349
VERSION     XP_034185349.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329458.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185349.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1161
                     /product="cyclin-G-associated kinase"
                     /calculated_mol_wt=127723
     Region          32..311
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(39..42,45,47,60,62,92,114..117,165,169..170,172,
                     182..183)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          403..573
                     /region_name="PTP_DSP_cys"
                     /note="cys-based protein tyrosine phosphatase and
                     dual-specificity phosphatase superfamily; cl28904"
                     /db_xref="CDD:475123"
     Region          579..700
                     /region_name="PTEN_C2"
                     /note="C2 domain of PTEN tumour-suppressor protein;
                     pfam10409"
                     /db_xref="CDD:463081"
     Region          <1107..1152
                     /region_name="DnaJ"
                     /note="DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock
                     protein 40) proteins are highly conserved and play crucial
                     roles in protein translation, folding, unfolding,
                     translocation, and degradation. They act primarily by
                     stimulating the ATPase activity of Hsp70s; cd06257"
                     /db_xref="CDD:99751"
     Site            order(1123..1125,1137,1140..1141,1144..1145)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..1161
                     /gene="aux"
                     /coded_by="XM_034329458.2:17..3502"
                     /db_xref="GeneID:117606678"
ORIGIN      
        1 msdylrsalg ylngatntne yvgqvleinn vklrvnrlla eggwalvfav edvntgkeya
       61 lkrliavdee anksivqeie tlkrlsghpn viqylyaqrl eredrkgyey llvtelcpgg
      121 tvadilrsls tntltlaqic kvayqatrav hhmhsqqpep fvhrdiklen fligtdglvk
      181 lcdfgststq qilpnpswna qrratledqm akyttpmyra pemmdtwnne pigppvdcws
      241 lgcilyslit lrhpfpegnk laivngkysp lpanprlacl hdivkgcldv spvrrlttsm
      301 ilerlaaiae snnfdprepp kievpaktpp pprpvpppst pappprpspp vnvppprpap
      361 vqtavakvpv aqstglfssl kggagsilrn lkdtsskvmh tvqqsmarte ldasyltsri
      421 limpypadgi esayranhve dvraflqarh pppariqlyn lsrgrpnvtr lpgrhidcsf
      481 ayatsdshap llsalyqicq diyrylnadf nhvvvlyctd gyrasatvac tlliyaralt
      541 tpeetislft trrcqppqlq pselrsinym sllssgvqph tkplalrslm iqpvplftra
      601 kdgsrpyidi ysngalvfst krpeyedmrl fgmmegkvsl algnatvrgd vtvvifharq
      661 qlgriigiki asvhfhtgyi pltentltfk kkdlddapei ggkfhvllni mvaeenskla
      721 rvpapweaev ttslipdalf gstlemeetl enfrtaqpee ipkevsatpl aeepsnvsvq
      781 eeeaepienv eeemkeeavp leadllnlgm pdtstlnspq ppvnpgldif ssssqnesdl
      841 lggfgvfpqe etkstvpkis dpaqndllfg hdqptkndnt nnnlnmffeq nqsnskqqvn
      901 dllfdplggt sannflggmp ssksstvkss tteesfprns svpnfaaqsk dpfanlassl
      961 gagltsswng tprnsntpqs aspapastpi hsspnthrsn vasgennmad tgassnkstk
     1021 gdafedllgs qgynffssrk aekdspktin qmrkveaakt mdpdrlkiae wtegkkgnlr
     1081 allcsmhtvl wpeadrwqrc emhqlvsaad vkkayrkacl avhpdkqagt aneniaklif
     1141 melnnawstf endasqqnlf s
//
LOCUS       XP_034185351             702 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 7 [Osmia lignaria lignaria].
ACCESSION   XP_034185351
VERSION     XP_034185351.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329460.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185351.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..702
                     /product="autophagy-related 7"
                     /calculated_mol_wt=78582
     Region          7..699
                     /region_name="E1_like_apg7"
                     /note="E1-like protein-activating enzyme Gsa7p/Apg7p;
                     TIGR01381"
                     /db_xref="CDD:273590"
     CDS             1..702
                     /gene="Atg7"
                     /coded_by="XM_034329460.2:606..2714"
                     /db_xref="GeneID:117606680"
ORIGIN      
        1 msnfvkfatl rsttdctfwa kfaelkidkf kldekavinl wgiyslesln eentnplimd
       61 ctsfnedtet fshnssvvcl ghmintntfe afrkidpekl idsigkdiih siqdgsilqk
      121 pwklslflvv aysdlkkyrf hywvahptpl klpemyyeen pkpinqeftn nsddlcrgfl
      181 rldsrsknyf tvliskedkm sivdlatgin tlemnkvdsn gsqeykelyf afydpcttaq
      241 pgwplrnllc lllwhcptyc ftkiikvisi rgnkaqnsiv fklktkeyed skkirnyvfg
      301 cslvgwerna agkmgptivd lshtmdpakl sdrainlnlk lmkwrlvpdl dlekvsnlrc
      361 lllgagtlgc avsrvllgwg vnsitfvdss nvshsntvrq slythedavk hkhkahaard
      421 allnirpsmn aegvvlhipm pghvvgesmm astiksleql eelveksdvv fllldsrear
      481 wlptvlcaak nkiainaalg fdsytvqrhg prnltvpssp dldtkhpcgs dlgcyfcndv
      541 tqpgnsqtdr tldqqctvsr pglsqiaagl avellaavtq hpqgieagal vgnsrdsitq
      601 ndtelvgllg cvphtirgsl wnydtqltit hrftactacs vpvimeyknr glsfvldacn
      661 ipnyleklsg lelilkrpdl edlcyaldni sddeeekkdd tp
//
LOCUS       XP_034185358             189 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606686 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185358
VERSION     XP_034185358.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329467.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="uncharacterized protein LOC117606686 isoform X1"
                     /calculated_mol_wt=20795
     Region          161..176
                     /region_name="TDU"
                     /note="Short repeats in human TONDU, fly vestigial and
                     other proteins; smart00711"
                     /db_xref="CDD:197839"
     CDS             1..189
                     /gene="LOC117606686"
                     /coded_by="XM_034329467.2:2961..3530"
                     /db_xref="GeneID:117606686"
ORIGIN      
        1 metknllivs dewrsegmev pskrrclwqp wlntdkpgee rpkatnlhrk pssawrkerr
       61 rdpiqseald msaarvhhhh hsrpsvivpt tpqpgttied tavtptpslt psaataggqr
      121 tgirtvggvs dpaidehfkr slgleeyaav fnatttdkea glsvddhfak algetwtrlq
      181 atsrskdst
//
LOCUS       XP_034185359             170 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606686 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185359
VERSION     XP_034185359.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329468.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..170
                     /product="uncharacterized protein LOC117606686 isoform X2"
                     /calculated_mol_wt=18243
     Region          142..157
                     /region_name="TDU"
                     /note="Short repeats in human TONDU, fly vestigial and
                     other proteins; smart00711"
                     /db_xref="CDD:197839"
     CDS             1..170
                     /gene="LOC117606686"
                     /coded_by="XM_034329468.2:386..898"
                     /db_xref="GeneID:117606686"
ORIGIN      
        1 msavesaldv lsraatmvqe erpkatnlhr kpssawrker rrdpiqseal dmsaarvhhh
       61 hhsrpsvivp ttpqpgttie dtavtptpsl tpsaataggq rtgirtvggv sdpaidehfk
      121 rslgleeyaa vfnatttdke aglsvddhfa kalgetwtrl qatsrskdst
//
LOCUS       XP_034190386            5175 aa            linear   INV 03-SEP-2025
DEFINITION  midasin [Osmia lignaria lignaria].
ACCESSION   XP_034190386
VERSION     XP_034190386.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334495.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190386.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..5175
                     /product="midasin"
                     /calculated_mol_wt=594580
     Region          281..398
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Region          432..535
                     /region_name="AAA_lid_7"
                     /note="Midasin AAA lid domain; pfam17867"
                     /db_xref="CDD:465540"
     Region          604..835
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Region          848..950
                     /region_name="AAA_lid_5"
                     /note="Midasin AAA lid domain; pfam17865"
                     /db_xref="CDD:407722"
     Region          1016..1152
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            1021..1028
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(1022..1029,1088,1133)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            1084..1089
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     Site            1147
                     /site_type="other"
                     /note="arginine finger"
                     /db_xref="CDD:99707"
     Region          1164..1268
                     /region_name="AAA_lid_7"
                     /note="Midasin AAA lid domain; pfam17867"
                     /db_xref="CDD:465540"
     Region          1325..1470
                     /region_name="AAA_5"
                     /note="AAA domain (dynein-related subfamily); pfam07728"
                     /db_xref="CDD:400191"
     Site            1330..1337
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(1331..1338,1402,1452)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            1398..1403
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     Site            1469
                     /site_type="other"
                     /note="arginine finger"
                     /db_xref="CDD:99707"
     Region          1653..1792
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            1658..1665
                     /site_type="other"
                     /note="Walker A motif"
                     /db_xref="CDD:99707"
     Site            order(1659..1666,1728)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:99707"
     Site            1724..1729
                     /site_type="other"
                     /note="Walker B motif"
                     /db_xref="CDD:99707"
     Region          1804..1903
                     /region_name="AAA_lid_7"
                     /note="Midasin AAA lid domain; pfam17867"
                     /db_xref="CDD:465540"
     Region          1968..2089
                     /region_name="AAA"
                     /note="ATPases associated with a variety of cellular
                     activities; smart00382"
                     /db_xref="CDD:214640"
     Region          <2125..2214
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Region          <4295..4827
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          4910..5170
                     /region_name="vWA_midasin"
                     /note="VWA_Midasin: Midasin is a member of the AAA ATPase
                     family. The proteins of this family are unified by their
                     common archetectural organization that is based upon a
                     conserved ATPase domain. The AAA domain of midasin
                     contains six tandem AAA protomers. The...; cd01460"
                     /db_xref="CDD:238737"
     Site            order(4976,4978,4980,5047,5078)
                     /site_type="active"
                     /note="metal ion dependent adhesion site (MIDAS) [active]"
                     /db_xref="CDD:238737"
     CDS             1..5175
                     /gene="LOC117608842"
                     /coded_by="XM_034334495.2:103..15630"
                     /db_xref="GeneID:117608842"
ORIGIN      
        1 mllqnlmtfi krkesyaqrl srfqvgqels kddckkviyi lreflmdlkc tediaecfpq
       61 llpalvssci stdtisfndg dsvhklnsvv lgkllnksrd llafvmqyfd vnpapfecve
      121 idpnrrnkrk vvsvvevsdy dlvtacydil csapthfkyi wnwskffkyl thdnesvkwv
      181 amnciaivlg msesccndfl pqnhqlcieh nkvhensvka tftkeiddif ddlpsvvsig
      241 gillplikry ksknqlslvp apsarrnlqn lvfavssnkc vclqgvvgsg kttlveylaq
      301 ttghdnrnfa kvqlgdqtds kmllgmyrct dipgeflwqp gilteavlsg kwllledidc
      361 aptdvisvvg qlvenktlsv pgyrdaihvk sgfqlfvtrr ltsgglpkpl qmsmpsqkhf
      421 scvnveplsk eelvtlvqti fpllntvatr ivdvfllfsv gnheddivns mrnarqistr
      481 dlikwcsrav vdfdvsspts alkvfqdald vfccsvpdqe qrlnsaisva hklgivktka
      541 eyfcqvhkps mnfhkgyliv graklkmkkc drvkleckns nfsytrpsic lleriascvi
      601 qkepvllvge tgtgktscvq ylaqitshkl vvinmnqqse stdllggykp vdlkllifpi
      661 reefeilfrs yfaiepnlkf lnhvgrcfee rkwktltslm vhstvaalnr lrasaknqte
      721 aelvkkwekm aekleklrsq vhsqyslafs fvegslvkal kngdwvllde inlataetle
      781 clsgllegsc gslsllergd kepikrhkdf aifacmnpat dvgkkdlpvg lrnrftefyv
      841 delteksdll llvnsylkdl nlppakiesi vkfylnvrke aetnlldgtg hkphyslrtl
      901 cralsvsaqn pcanalrsly eafclsfltq ldsksyplvq kmivktllgn ravkaiigsp
      961 iprtqsgpad hficfegywi pkgdlepevp edyiltetvk qnlkdlvrvv sigkmpvllq
     1021 gdtsvgktsl itylakasgh tcvrinnheh tdlqeyvgty vadssgklvf kegilvtamr
     1081 kghwiildel nlapsdvlea lnrvlddnre lfipeiqqav kahdnfmlfa tqnppglygg
     1141 rkvlsrafrn rfvelhfdei pakelqvilh krckmpktyc rqiinvmtel qirrkstatf
     1201 agkkgfitlr dlfrwaeryr lapdlgttly dwsqhladeg ylvlsskvrr peecleiiev
     1261 lkkhlrrdvd pdtlftlske tssvtrpilk sllskkipgf khvvwtyqmr kmavllskac
     1321 efnepvllig etgggkttvc qllsvirrqk lsivnchmht easdflgslr pvrehkenrk
     1381 lfewvdgplv kamksggffl vdeisladds vlerlnslle perklliaeq ssaeenatvt
     1441 ahenfifvgt mnpggdygkk elspalrnrf teiwcegsas fddlqsimih nlreefkesv
     1501 sagiieflkw lqateigkkl vvsvrdiltw vnfinccknm eigdafyhga alsyidglgs
     1561 gltavenskn lkdfkksslk fiehqvqttl ksklllnceg fdvedredef ginpfyvkkg
     1621 nekissnigf afkspttrmn tlkvlralql qkpillegsp gvgktslvsa lakasghrll
     1681 rlnlsdqtdi sdlfgadlpv eggkpgefsw tdgpflramk ngdwilldel nlasqsvleg
     1741 lnacldhrge vyipelgmtf kvkpgtrlfg cqnplrqgga rrglpksfln rftqvfvdtl
     1801 eqkdlkfilk tqfpelpeql ldkmiqfnee issqagitwa hqgspwemnl rdvtrwceit
     1861 maaftrnskk synpgngakl iyvdrmrtne dknnvieiyn rifsptdypl pdpnfpvhvt
     1921 kdevilgdei lnrsdfsiye dddllllreq kavlrslaqc vnmnwmsilv ggsgtgkssl
     1981 vhllanlagq klksivinsa mdtteilggf eqtdydrhfe elldrtetll igilrnnlmk
     2041 ehldlvayyh slldnvrhls igdekrmett mstetklflr ktvelsklvs amkafgtfce
     2101 levqeiekkl tdlsiavekd kclnaggkfe wvnsvlvkcl qngtwllldh vnlcspaild
     2161 rlngllepng altigekgvd engnvftvka hrnfrlflim dprygeisra mrnrgveisi
     2221 ptpqhvlpcn pldvasllnn ygirklehqk livkihesly dkqyklnemv qvasftsqqv
     2281 akglpfedsl rnsyreicgt fdsrskkeal rkideilsen raeqvsfsyd ldpltlricd
     2341 lrrnaglavv rqqgfllqsa vekfknsskn idlqdlfdkp pivnamaflk nllldfyert
     2401 slddlsirri wncnifsknq lnrfevlnec lskeveslrp lfeknsaldi plnlvepstl
     2461 vnnlaivlyf rliifncqae enfqrnknvi ylkeyssavc lgklasnlkn eplilkfiel
     2521 ieqsakvidm vfrvdnlsin dkkyielrkd lkwfqrfynl gtielvdksk giffdlreis
     2581 sslkvhykwl nkffkklfql ldvkrcnssv ekkltnfskk flnlmvdadk frkirkkvkk
     2641 llpipsphfs edsmsfhenl ravlkkleiv eedlvdlskt rlkivsvgle enltarervi
     2701 nicihnfknd etnktedlse vdklgakite lkelddnsia tinnsteiei wpiyefffml
     2761 ftyrfqrklc eeirgnleqn vgriyeetvf rkfeevptip ihlialiksl nsdeilskrr
     2821 isllpelfss teefsersfa vknsdlllhw ydhretdced slsifqpnfi dkatlcnlif
     2881 elfltntgnr keminfgnyk nqkkllktiq tllwknslvl nsnefsistn dsvllefyin
     2941 yyttainkvt dsfaltlnee eqrdlnnrff epikllleik enlknennvl ergkgwlifg
     3001 hlqififhdl gsidpvqkva lkskymkeni sdlerlmcie nlqctilgin lskerkqsrv
     3061 drlkelsnem ksfedfhavr ptltfqnlre eienfsknig sfesifkhvt hlndtielec
     3121 dkkesfnqir eklqeaviwk kslerfsgkl eknflngfpd lvspmifais nlhhgisifi
     3181 helsnmqart snsdicynll rfpvtgvcqq nyiniaklcs seklifdlse dkssksiqky
     3241 rfkmaniale elenfsllav drsqsfwgel nkillkiril wkdqeerree emkekqslfr
     3301 tkfeskeede ldfrkifpgy qqdflelaem fpgyqqdfpe lqripetvqn eddsfrelis
     3361 ikdarrvqei hwnilrnser penslkpsfi kplaerfemf gstirtypen hssklicsln
     3421 vlisqkstfd nrksytevyd fyrdsnipei edcqplfdgl agrirdllke wpenpilnsi
     3481 kliidrifsf svdsslsrfl vgaellltki qqweenarsd vslsgfiesf gekilrwrkl
     3541 eisrwkdsle vledhlktda skwwfflfdl vdqylaedie esekegelkd sqttenvske
     3601 kiienlgrfl tespliefet rlelvymfyc hvsyfketgk qkelsaifwn iyfyytqfln
     3661 dvqnkikvlk epiakklkdt ikitrwndis ywsvrntaek trrtlhkfvk efqkglgenv
     3721 asylslkgkv gtaetftrre lgpseflmdl egkklkiekl tqkarqycey iiiksnylei
     3781 rkdietfied sieesnrlkn mevdktltkg kqqsqlksml qqkkmalany fktlsrlgis
     3841 yrigvltwkn rkeeinnfaa tpldlkeiqr ftliarkkte nvekglieew sscdkyyyes
     3901 liklnflnai ldsgksdigm qnaercrgls ihlvllahrq ketiadffqh flpfriqisn
     3961 lalmledski csqiskemer inipkqkslq iimsnfqell ealqmsmeqi lvylescpme
     4021 slaeenldln etaipvlken nicrnsitwm qnslnlikti tnelhswmmi skrveksgqi
     4081 flyhqrhidf lqtgyqkvte ikeklvdlki tftvdhpiae nlnflidkid egnnllkstl
     4141 atvveetnep kimeeyeekl dvlitqillv iqkkiqgnqa sseetidsqk edfeenlvte
     4201 klieslkkni snlrlnkisq ifgellqiif esdvesankc vrllvqhlpl leqytlflhy
     4261 ylheqvasfr ltckflylql nvfldlarng fcqpentdas eddqeggtae ggmglaegeg
     4321 qkdvsekies edqledtkgp neeekdedkr dmkeeekgie msenfdshlq nlekgededk
     4381 dkeeeedldk emgetdenae kldeeiwkde deeeennden eddkkeekgs gektgqeels
     4441 akdgdenkne neeqerdseg qrkeeekkei nefeepevdd dqidpyhgkh qpeiepesfd
     4501 lpeemnldee mkednpenee npfdidqmke tkvpeekede eaekqedlne deqqdssade
     4561 ddqekeedsn mepdkrrder drnveedkke enmeeekele edkaqasida gskevdasqe
     4621 veskkdgsrd avesqlnnen vetdstdnia ddkkdkgtgq sqtekreghs gskqeenapv
     4681 sgeeksnpvk krrkpgesde nrsllddevq psikkmktap trqeldnnee kennevgges
     4741 kvyehikste kfddyvfdaa teeqvrkqas nmddkleeee eeeddikmhd dtvadnqeei
     4801 nkehpekvne skkdkstnar gersaddveq mevggevege vvettrvlrg nestvhtkls
     4861 eievydafvd niaekrseie nmlgqwsrep ssieaanaws sisaltesaa relseklrlv
     4921 leptltsrlk gdyrtgrrin mrkvipyias efrkdkiwlr rtkpskrdyq ivlaiddsss
     4981 madnhskela feslsligka mtylevgqla vinfgervni lhpfgenftd esgarliqem
     5041 kfeqkktmig qlldftidmf tsqssssdna kllailsdgr gifsegiekv taavrrskll
     5101 nvflvfiivd nplnkdsild irmpifengk llgirsymdn fpfpfyiilr dldslpivls
     5161 dalrqwfeiv gridt
//
LOCUS       XP_076545940             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608848 [Osmia lignaria lignaria].
ACCESSION   XP_076545940
VERSION     XP_076545940.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689825.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117608848"
                     /calculated_mol_wt=84771
     CDS             1..736
                     /gene="LOC117608848"
                     /coded_by="XM_076689825.1:8159..10369"
                     /db_xref="GeneID:117608848"
ORIGIN      
        1 miefeaecle edpeareffe tlnrsrkplk danlkdeeag gpsmiveesk eksrefvapp
       61 sevameqgkg gtpgkkkgnk kkestdhsvk stanvrigle esrkvrkraa eeeteineeg
      121 skrdkkegee fvvdnsplag akrattpvrq vgtplkdkte getiegiaek daiplgrktn
      181 rriergegnq fyveelrske ymitmvlgre ctskfkrnni vkiykilgsr srflrglkmv
      241 gfnkaeitfs dmaaanailk maeqegagfe aflperkktr kgviteweds iedledfimp
      301 gqgefkferm kkkvvkdgks vwtegvailv tvkgdrlptd lkigaghval rifpyveqvk
      361 qcykcfaygh vqmqcrslkk clvclgrehg acdktpvcln cggnhtslsk tcwihqrekl
      421 irkvmayknv ayatakgiva sqlgenwdee dlgrdagsrd ypmlptrrte wweakevpme
      481 delellkeak kkvegdrvgi rrwesfsenm rrkqaggrsr grgrlrleey avedrsprtq
      541 gvtinvanrf dslqqeevet gqkeaeyvfg eglpveraik rmskmeqraq eriigqlekk
      601 rdeeflsdlm klikekgyeq evermlndnk redqrtedee yaywnttrhr pnwdipipek
      661 treklrrrke kyeaqkaake aqeqtledia vrahqqygwt keranqeddy rrgrledlmi
      721 riegrvcgnr etfnde
//
LOCUS       XP_076545942             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608848 [Osmia lignaria lignaria].
ACCESSION   XP_076545942
VERSION     XP_076545942.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689827.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117608848"
                     /calculated_mol_wt=84771
     CDS             1..736
                     /gene="LOC117608848"
                     /coded_by="XM_076689827.1:8141..10351"
                     /db_xref="GeneID:117608848"
ORIGIN      
        1 miefeaecle edpeareffe tlnrsrkplk danlkdeeag gpsmiveesk eksrefvapp
       61 sevameqgkg gtpgkkkgnk kkestdhsvk stanvrigle esrkvrkraa eeeteineeg
      121 skrdkkegee fvvdnsplag akrattpvrq vgtplkdkte getiegiaek daiplgrktn
      181 rriergegnq fyveelrske ymitmvlgre ctskfkrnni vkiykilgsr srflrglkmv
      241 gfnkaeitfs dmaaanailk maeqegagfe aflperkktr kgviteweds iedledfimp
      301 gqgefkferm kkkvvkdgks vwtegvailv tvkgdrlptd lkigaghval rifpyveqvk
      361 qcykcfaygh vqmqcrslkk clvclgrehg acdktpvcln cggnhtslsk tcwihqrekl
      421 irkvmayknv ayatakgiva sqlgenwdee dlgrdagsrd ypmlptrrte wweakevpme
      481 delellkeak kkvegdrvgi rrwesfsenm rrkqaggrsr grgrlrleey avedrsprtq
      541 gvtinvanrf dslqqeevet gqkeaeyvfg eglpveraik rmskmeqraq eriigqlekk
      601 rdeeflsdlm klikekgyeq evermlndnk redqrtedee yaywnttrhr pnwdipipek
      661 treklrrrke kyeaqkaake aqeqtledia vrahqqygwt keranqeddy rrgrledlmi
      721 riegrvcgnr etfnde
//
LOCUS       XP_076545943             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608848 [Osmia lignaria lignaria].
ACCESSION   XP_076545943
VERSION     XP_076545943.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689828.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117608848"
                     /calculated_mol_wt=84771
     CDS             1..736
                     /gene="LOC117608848"
                     /coded_by="XM_076689828.1:8182..10392"
                     /db_xref="GeneID:117608848"
ORIGIN      
        1 miefeaecle edpeareffe tlnrsrkplk danlkdeeag gpsmiveesk eksrefvapp
       61 sevameqgkg gtpgkkkgnk kkestdhsvk stanvrigle esrkvrkraa eeeteineeg
      121 skrdkkegee fvvdnsplag akrattpvrq vgtplkdkte getiegiaek daiplgrktn
      181 rriergegnq fyveelrske ymitmvlgre ctskfkrnni vkiykilgsr srflrglkmv
      241 gfnkaeitfs dmaaanailk maeqegagfe aflperkktr kgviteweds iedledfimp
      301 gqgefkferm kkkvvkdgks vwtegvailv tvkgdrlptd lkigaghval rifpyveqvk
      361 qcykcfaygh vqmqcrslkk clvclgrehg acdktpvcln cggnhtslsk tcwihqrekl
      421 irkvmayknv ayatakgiva sqlgenwdee dlgrdagsrd ypmlptrrte wweakevpme
      481 delellkeak kkvegdrvgi rrwesfsenm rrkqaggrsr grgrlrleey avedrsprtq
      541 gvtinvanrf dslqqeevet gqkeaeyvfg eglpveraik rmskmeqraq eriigqlekk
      601 rdeeflsdlm klikekgyeq evermlndnk redqrtedee yaywnttrhr pnwdipipek
      661 treklrrrke kyeaqkaake aqeqtledia vrahqqygwt keranqeddy rrgrledlmi
      721 riegrvcgnr etfnde
//
LOCUS       XP_076545944             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608848 [Osmia lignaria lignaria].
ACCESSION   XP_076545944
VERSION     XP_076545944.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689829.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117608848"
                     /calculated_mol_wt=84771
     CDS             1..736
                     /gene="LOC117608848"
                     /coded_by="XM_076689829.1:6338..8548"
                     /db_xref="GeneID:117608848"
ORIGIN      
        1 miefeaecle edpeareffe tlnrsrkplk danlkdeeag gpsmiveesk eksrefvapp
       61 sevameqgkg gtpgkkkgnk kkestdhsvk stanvrigle esrkvrkraa eeeteineeg
      121 skrdkkegee fvvdnsplag akrattpvrq vgtplkdkte getiegiaek daiplgrktn
      181 rriergegnq fyveelrske ymitmvlgre ctskfkrnni vkiykilgsr srflrglkmv
      241 gfnkaeitfs dmaaanailk maeqegagfe aflperkktr kgviteweds iedledfimp
      301 gqgefkferm kkkvvkdgks vwtegvailv tvkgdrlptd lkigaghval rifpyveqvk
      361 qcykcfaygh vqmqcrslkk clvclgrehg acdktpvcln cggnhtslsk tcwihqrekl
      421 irkvmayknv ayatakgiva sqlgenwdee dlgrdagsrd ypmlptrrte wweakevpme
      481 delellkeak kkvegdrvgi rrwesfsenm rrkqaggrsr grgrlrleey avedrsprtq
      541 gvtinvanrf dslqqeevet gqkeaeyvfg eglpveraik rmskmeqraq eriigqlekk
      601 rdeeflsdlm klikekgyeq evermlndnk redqrtedee yaywnttrhr pnwdipipek
      661 treklrrrke kyeaqkaake aqeqtledia vrahqqygwt keranqeddy rrgrledlmi
      721 riegrvcgnr etfnde
//
LOCUS       XP_076545945             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608848 [Osmia lignaria lignaria].
ACCESSION   XP_076545945
VERSION     XP_076545945.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689830.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117608848"
                     /calculated_mol_wt=84771
     CDS             1..736
                     /gene="LOC117608848"
                     /coded_by="XM_076689830.1:8189..10399"
                     /db_xref="GeneID:117608848"
ORIGIN      
        1 miefeaecle edpeareffe tlnrsrkplk danlkdeeag gpsmiveesk eksrefvapp
       61 sevameqgkg gtpgkkkgnk kkestdhsvk stanvrigle esrkvrkraa eeeteineeg
      121 skrdkkegee fvvdnsplag akrattpvrq vgtplkdkte getiegiaek daiplgrktn
      181 rriergegnq fyveelrske ymitmvlgre ctskfkrnni vkiykilgsr srflrglkmv
      241 gfnkaeitfs dmaaanailk maeqegagfe aflperkktr kgviteweds iedledfimp
      301 gqgefkferm kkkvvkdgks vwtegvailv tvkgdrlptd lkigaghval rifpyveqvk
      361 qcykcfaygh vqmqcrslkk clvclgrehg acdktpvcln cggnhtslsk tcwihqrekl
      421 irkvmayknv ayatakgiva sqlgenwdee dlgrdagsrd ypmlptrrte wweakevpme
      481 delellkeak kkvegdrvgi rrwesfsenm rrkqaggrsr grgrlrleey avedrsprtq
      541 gvtinvanrf dslqqeevet gqkeaeyvfg eglpveraik rmskmeqraq eriigqlekk
      601 rdeeflsdlm klikekgyeq evermlndnk redqrtedee yaywnttrhr pnwdipipek
      661 treklrrrke kyeaqkaake aqeqtledia vrahqqygwt keranqeddy rrgrledlmi
      721 riegrvcgnr etfnde
//
LOCUS       XP_076545946             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608848 [Osmia lignaria lignaria].
ACCESSION   XP_076545946
VERSION     XP_076545946.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689831.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117608848"
                     /calculated_mol_wt=84771
     CDS             1..736
                     /gene="LOC117608848"
                     /coded_by="XM_076689831.1:7441..9651"
                     /db_xref="GeneID:117608848"
ORIGIN      
        1 miefeaecle edpeareffe tlnrsrkplk danlkdeeag gpsmiveesk eksrefvapp
       61 sevameqgkg gtpgkkkgnk kkestdhsvk stanvrigle esrkvrkraa eeeteineeg
      121 skrdkkegee fvvdnsplag akrattpvrq vgtplkdkte getiegiaek daiplgrktn
      181 rriergegnq fyveelrske ymitmvlgre ctskfkrnni vkiykilgsr srflrglkmv
      241 gfnkaeitfs dmaaanailk maeqegagfe aflperkktr kgviteweds iedledfimp
      301 gqgefkferm kkkvvkdgks vwtegvailv tvkgdrlptd lkigaghval rifpyveqvk
      361 qcykcfaygh vqmqcrslkk clvclgrehg acdktpvcln cggnhtslsk tcwihqrekl
      421 irkvmayknv ayatakgiva sqlgenwdee dlgrdagsrd ypmlptrrte wweakevpme
      481 delellkeak kkvegdrvgi rrwesfsenm rrkqaggrsr grgrlrleey avedrsprtq
      541 gvtinvanrf dslqqeevet gqkeaeyvfg eglpveraik rmskmeqraq eriigqlekk
      601 rdeeflsdlm klikekgyeq evermlndnk redqrtedee yaywnttrhr pnwdipipek
      661 treklrrrke kyeaqkaake aqeqtledia vrahqqygwt keranqeddy rrgrledlmi
      721 riegrvcgnr etfnde
//
LOCUS       XP_076545947             736 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608848 [Osmia lignaria lignaria].
ACCESSION   XP_076545947
VERSION     XP_076545947.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689832.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="uncharacterized protein LOC117608848"
                     /calculated_mol_wt=84771
     CDS             1..736
                     /gene="LOC117608848"
                     /coded_by="XM_076689832.1:2725..4935"
                     /db_xref="GeneID:117608848"
ORIGIN      
        1 miefeaecle edpeareffe tlnrsrkplk danlkdeeag gpsmiveesk eksrefvapp
       61 sevameqgkg gtpgkkkgnk kkestdhsvk stanvrigle esrkvrkraa eeeteineeg
      121 skrdkkegee fvvdnsplag akrattpvrq vgtplkdkte getiegiaek daiplgrktn
      181 rriergegnq fyveelrske ymitmvlgre ctskfkrnni vkiykilgsr srflrglkmv
      241 gfnkaeitfs dmaaanailk maeqegagfe aflperkktr kgviteweds iedledfimp
      301 gqgefkferm kkkvvkdgks vwtegvailv tvkgdrlptd lkigaghval rifpyveqvk
      361 qcykcfaygh vqmqcrslkk clvclgrehg acdktpvcln cggnhtslsk tcwihqrekl
      421 irkvmayknv ayatakgiva sqlgenwdee dlgrdagsrd ypmlptrrte wweakevpme
      481 delellkeak kkvegdrvgi rrwesfsenm rrkqaggrsr grgrlrleey avedrsprtq
      541 gvtinvanrf dslqqeevet gqkeaeyvfg eglpveraik rmskmeqraq eriigqlekk
      601 rdeeflsdlm klikekgyeq evermlndnk redqrtedee yaywnttrhr pnwdipipek
      661 treklrrrke kyeaqkaake aqeqtledia vrahqqygwt keranqeddy rrgrledlmi
      721 riegrvcgnr etfnde
//
LOCUS       XP_034190436             496 aa            linear   INV 03-SEP-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190436
VERSION     XP_034190436.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334545.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="hepatocyte nuclear factor 4 isoform X1"
                     /calculated_mol_wt=54664
     Region          72..147
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(72,75,89,92,108,114,124,127)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(78,82..84,90..91,93,95,97..98,101,121..122,125,128,
                     141..143,145)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(109,122..123,125)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          167..403
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(204,210,214,248,252,255,265..266,294,384..385,388)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(219,223,233..234,236..237,240..241,398..399,
                     402..403)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(301,322,328,342..343,346..347,350..351,361..362,
                     364..366,368..369,371..373,375..376,379)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..496
                     /gene="Hnf4"
                     /coded_by="XM_034334545.2:119..1609"
                     /db_xref="GeneID:117608855"
ORIGIN      
        1 mclcyfcalt rddstlddyh elfetkvnrk mrfddqlfev fdadlsmgag aaggsvlglp
       61 gsgvggvlsq hcaicgdrat gkhygaascd gckgffrrsv rknhlytcrf srncvvdkdk
      121 rnqcrycrlr kcfkagmkke avqnerdris crrpsyeeqt sngsglsvvs llqaemlsrq
      181 vgaalelgsp gndidlstkq ianindvcds mkqqllilve wakyipafse ltlddqvall
      241 rahagehlll gvarrsmqlk dvlllgnnci itkncpvisl stegrnqdld iskvgirvmd
      301 elvkplnevq iddtefaclk aivffdpnak glsepqrikq lryqiqinle dyisdrqyds
      361 rgrfgeillt lpalqsiswq mieqiqfvrl fgvahidnll qemllggata eingaatpip
      421 isnapgsyvs snespssplt panapplspq dhmlaggspv milrdltpiq sqedvtsatg
      481 frmfkqepsl esestf
//
LOCUS       XP_034190437             490 aa            linear   INV 03-SEP-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190437
VERSION     XP_034190437.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334546.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="hepatocyte nuclear factor 4 isoform X2"
                     /calculated_mol_wt=54063
     Region          72..147
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(72,75,89,92,108,114,124,127)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(78,82..84,90..91,93,95,97..98,101,121..122,125,128,
                     141..143,145)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(109,122..123,125)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          167..397
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(204,210,214,248,252,255,265..266,288,378..379,382)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(219,223,233..234,236..237,240..241,392..393,
                     396..397)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(295,316,322,336..337,340..341,344..345,355..356,
                     358..360,362..363,365..367,369..370,373)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..490
                     /gene="Hnf4"
                     /coded_by="XM_034334546.2:118..1590"
                     /db_xref="GeneID:117608855"
ORIGIN      
        1 mclcyfcalt rddstlddyh elfetkvnrk mrfddqlfev fdadlsmgag aaggsvlglp
       61 gsgvggvlsq hcaicgdrat gkhygaascd gckgffrrsv rknhlytcrf srncvvdkdk
      121 rnqcrycrlr kcfkagmkke avqnerdris crrpsyeeqt sngsglsvvs llqaemlsrq
      181 vgaalelgsp gndidlstkq ianindvcds mkqqllilve wakyipafse ltlddqvall
      241 rahagehlll gvarrsmqlk dvlllgnnci itkncpegrn qdldiskvgi rvmdelvkpl
      301 nevqiddtef aclkaivffd pnakglsepq rikqlryqiq inledyisdr qydsrgrfge
      361 illtlpalqs iswqmieqiq fvrlfgvahi dnllqemllg gataeingaa tpipisnapg
      421 syvssnesps spltpanapp lspqdhmlag gspvmilrdl tpiqsqedvt satgfrmfkq
      481 epslesestf
//
LOCUS       XP_034190438             476 aa            linear   INV 03-SEP-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190438
VERSION     XP_034190438.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334547.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190438.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="hepatocyte nuclear factor 4 isoform X3"
                     /calculated_mol_wt=51857
     Region          52..127
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(52,55,69,72,88,94,104,107)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(58,62..64,70..71,73,75,77..78,81,101..102,105,108,
                     121..123,125)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(89,102..103,105)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          147..383
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(184,190,194,228,232,235,245..246,274,364..365,368)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(199,203,213..214,216..217,220..221,378..379,
                     382..383)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(281,302,308,322..323,326..327,330..331,341..342,
                     344..346,348..349,351..353,355..356,359)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..476
                     /gene="Hnf4"
                     /coded_by="XM_034334547.2:417..1847"
                     /db_xref="GeneID:117608855"
ORIGIN      
        1 meerpenneg psvnssssst ddadlsmgag aaggsvlglp gsgvggvlsq hcaicgdrat
       61 gkhygaascd gckgffrrsv rknhlytcrf srncvvdkdk rnqcrycrlr kcfkagmkke
      121 avqnerdris crrpsyeeqt sngsglsvvs llqaemlsrq vgaalelgsp gndidlstkq
      181 ianindvcds mkqqllilve wakyipafse ltlddqvall rahagehlll gvarrsmqlk
      241 dvlllgnnci itkncpvisl stegrnqdld iskvgirvmd elvkplnevq iddtefaclk
      301 aivffdpnak glsepqrikq lryqiqinle dyisdrqyds rgrfgeillt lpalqsiswq
      361 mieqiqfvrl fgvahidnll qemllggata eingaatpip isnapgsyvs snespssplt
      421 panapplspq dhmlaggspv milrdltpiq sqedvtsatg frmfkqepsl esestf
//
LOCUS       XP_034190439             459 aa            linear   INV 03-SEP-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034190439
VERSION     XP_034190439.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334548.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="hepatocyte nuclear factor 4 isoform X4"
                     /calculated_mol_wt=49994
     Region          35..110
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(35,38,52,55,71,77,87,90)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(41,45..47,53..54,56,58,60..61,64,84..85,88,91,
                     104..106,108)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(72,85..86,88)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          130..366
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(167,173,177,211,215,218,228..229,257,347..348,351)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(182,186,196..197,199..200,203..204,361..362,
                     365..366)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(264,285,291,305..306,309..310,313..314,324..325,
                     327..329,331..332,334..336,338..339,342)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..459
                     /gene="Hnf4"
                     /coded_by="XM_034334548.2:344..1723"
                     /db_xref="GeneID:117608855"
ORIGIN      
        1 mgladadlsm gagaaggsvl glpgsgvggv lsqhcaicgd ratgkhygaa scdgckgffr
       61 rsvrknhlyt crfsrncvvd kdkrnqcryc rlrkcfkagm kkeavqnerd riscrrpsye
      121 eqtsngsgls vvsllqaeml srqvgaalel gspgndidls tkqianindv cdsmkqqlli
      181 lvewakyipa fseltlddqv allrahageh lllgvarrsm qlkdvlllgn nciitkncpv
      241 islstegrnq dldiskvgir vmdelvkpln evqiddtefa clkaivffdp nakglsepqr
      301 ikqlryqiqi nledyisdrq ydsrgrfgei lltlpalqsi swqmieqiqf vrlfgvahid
      361 nllqemllgg ataeingaat pipisnapgs yvssnespss pltpanappl spqdhmlagg
      421 spvmilrdlt piqsqedvts atgfrmfkqe pslesestf
//
LOCUS       XP_034190440             457 aa            linear   INV 03-SEP-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034190440
VERSION     XP_034190440.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334549.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="hepatocyte nuclear factor 4 isoform X5"
                     /calculated_mol_wt=49851
     Region          33..108
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(33,36,50,53,69,75,85,88)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(39,43..45,51..52,54,56,58..59,62,82..83,86,89,
                     102..104,106)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(70,83..84,86)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          128..364
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(165,171,175,209,213,216,226..227,255,345..346,349)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(180,184,194..195,197..198,201..202,359..360,
                     363..364)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(262,283,289,303..304,307..308,311..312,322..323,
                     325..327,329..330,332..334,336..337,340)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..457
                     /gene="Hnf4"
                     /coded_by="XM_034334549.2:16..1389"
                     /db_xref="GeneID:117608855"
ORIGIN      
        1 mvnadlsmga gaaggsvlgl pgsgvggvls qhcaicgdra tgkhygaasc dgckgffrrs
       61 vrknhlytcr fsrncvvdkd krnqcrycrl rkcfkagmkk eavqnerdri scrrpsyeeq
      121 tsngsglsvv sllqaemlsr qvgaalelgs pgndidlstk qianindvcd smkqqllilv
      181 ewakyipafs eltlddqval lrahagehll lgvarrsmql kdvlllgnnc iitkncpvis
      241 lstegrnqdl diskvgirvm delvkplnev qiddtefacl kaivffdpna kglsepqrik
      301 qlryqiqinl edyisdrqyd srgrfgeill tlpalqsisw qmieqiqfvr lfgvahidnl
      361 lqemllggat aeingaatpi pisnapgsyv ssnespsspl tpanapplsp qdhmlaggsp
      421 vmilrdltpi qsqedvtsat gfrmfkqeps lesestf
//
LOCUS       XP_034190442             450 aa            linear   INV 03-SEP-2025
DEFINITION  hepatocyte nuclear factor 4 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034190442
VERSION     XP_034190442.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334551.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="hepatocyte nuclear factor 4 isoform X6"
                     /calculated_mol_wt=49120
     Region          26..101
                     /region_name="NR_DBD_HNF4A"
                     /note="DNA-binding domain of heptocyte nuclear factor 4
                     (HNF4) is composed of two C4-type zinc fingers; cd06960"
                     /db_xref="CDD:143518"
     Site            order(26,29,43,46,62,68,78,81)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143518"
     Site            order(32,36..38,44..45,47,49,51..52,55,75..76,79,82,
                     95..97,99)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143518"
     Site            order(63,76..77,79)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:143518"
     Region          121..357
                     /region_name="NR_LBD_HNF4_like"
                     /note="The ligand binding domain of heptocyte nuclear
                     factor 4, which is explosively expanded in nematodes;
                     cd06931"
                     /db_xref="CDD:132729"
     Site            order(158,164,168,202,206,209,219..220,248,338..339,342)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132729"
     Site            order(173,177,187..188,190..191,194..195,352..353,
                     356..357)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132729"
     Site            order(255,276,282,296..297,300..301,304..305,315..316,
                     318..320,322..323,325..327,329..330,333)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:132729"
     CDS             1..450
                     /gene="Hnf4"
                     /coded_by="XM_034334551.2:67..1419"
                     /db_xref="GeneID:117608855"
ORIGIN      
        1 mgagaaggsv lglpgsgvgg vlsqhcaicg dratgkhyga ascdgckgff rrsvrknhly
       61 tcrfsrncvv dkdkrnqcry crlrkcfkag mkkeavqner driscrrpsy eeqtsngsgl
      121 svvsllqaem lsrqvgaale lgspgndidl stkqianind vcdsmkqqll ilvewakyip
      181 afseltlddq vallrahage hlllgvarrs mqlkdvlllg nnciitkncp vislstegrn
      241 qdldiskvgi rvmdelvkpl nevqiddtef aclkaivffd pnakglsepq rikqlryqiq
      301 inledyisdr qydsrgrfge illtlpalqs iswqmieqiq fvrlfgvahi dnllqemllg
      361 gataeingaa tpipisnapg syvssnesps spltpanapp lspqdhmlag gspvmilrdl
      421 tpiqsqedvt satgfrmfkq epslesestf
//
LOCUS       XP_034190443             495 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608856 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190443
VERSION     XP_034190443.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334552.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="uncharacterized protein LOC117608856 isoform X1"
                     /calculated_mol_wt=53803
     Region          35..345
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          35..60
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410626"
     Region          67..91
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(90..91,101..106,108..109,112,163,165..169,199,
                     202..204,206..208,210..211,307,310..311,313..314,317,
                     323..324,326..328,331,334..335)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          150..169
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410626"
     Region          199..242
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          268..317
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     Region          324..345
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..495
                     /gene="LOC117608856"
                     /coded_by="XM_034334552.2:3..1490"
                     /db_xref="GeneID:117608856"
ORIGIN      
        1 mamiganlrf gigasgnvss natkvfqchp ggateatlif slgalgmgan fllmaliltk
       61 rqlrrwsqgl lfhqamvdca raaillplgs silncqpmtk cslvetafll lvtvstvnml
      121 ttvlndspif petdeeadlt apllmdspqc vlfgtfmiwf asitinlgpt flsgalaant
      181 esghnapscp lvhgpfrhyi lnvlwiainm icvaltlihl rklhkdltka nveavkvall
      241 tilvnttgnq qtnavsenst sangtpkrsg aaeehrkmrn ylkrmeregv qrvkmflvit
      301 aaygifwgpl ffvtlvdhps ignptgyevt lhiayvhafv npvlflslhr glrqglsesf
      361 cgcceviasw ilgpsmpien iqppryqepi davpsppepp lppattcpdl tdvallkppl
      421 pptgkhlldd tvivppdpwt lvsrskstss vynedvsrrs silipppltm ndtksmkrff
      481 vynsesrksv hgsqv
//
LOCUS       XP_034190444             487 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608856 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190444
VERSION     XP_034190444.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334553.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..487
                     /product="uncharacterized protein LOC117608856 isoform X2"
                     /calculated_mol_wt=52661
     Region          35..345
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          35..60
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410626"
     Region          67..91
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(90..91,101..106,108..109,112,163,165..169,199,
                     202..204,206..208,210..211,307,310..311,313..314,317,
                     323..324,326..328,331,334..335)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          150..169
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410626"
     Region          199..242
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          268..317
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     Region          324..345
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..487
                     /gene="LOC117608856"
                     /coded_by="XM_034334553.2:3..1466"
                     /db_xref="GeneID:117608856"
ORIGIN      
        1 mamiganlrf gigasgnvss natkvfqchp ggateatlif slgalgmgan fllmaliltk
       61 rqlrrwsqgl lfhqamvdca raaillplgs silncqpmtk cslvetafll lvtvstvnml
      121 ttvlndspif petdeeadlt apllmdspqc vlfgtfmiwf asitinlgpt flsgalaant
      181 esghnapscp lvhgpfrhyi lnvlwiainm icvaltlihl rklhkdltka nveavkvall
      241 tilvnttgnq qtnavsenst sangtpkrsg aaeehrkmrn ylkrmeregv qrvkmflvit
      301 aaygifwgpl ffvtlvdhps ignptgyevt lhiayvhafv npvlflslhr glrqglsesf
      361 cgcceviasw ilgpsmpien iqppryqepi davpsppepp lppattcpdl tdvallkppl
      421 pptgkhlldd tvivppdpwt lvsrskstss vynedvsrrs silipppltm ndtkvnvspt
      481 ssdlpse
//
LOCUS       XP_034190445             462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608856 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190445
VERSION     XP_034190445.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334554.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="uncharacterized protein LOC117608856 isoform X3"
                     /calculated_mol_wt=50449
     Region          35..312
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          35..60
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410626"
     Region          67..91
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(90..91,101..106,108..109,112,163,165..169,194,
                     197..199,201..203,205..206,274,277..278,280..281,284,
                     290..291,293..295,298,301..302)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          150..169
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410626"
     Region          194..219
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          244..284
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     Region          291..312
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..462
                     /gene="LOC117608856"
                     /coded_by="XM_034334554.2:3..1391"
                     /db_xref="GeneID:117608856"
ORIGIN      
        1 mamiganlrf gigasgnvss natkvfqchp ggateatlif slgalgmgan fllmaliltk
       61 rqlrrwsqgl lfhqamvdca raaillplgs silncqpmtk cslvetafll lvtvstvnml
      121 ttvlndspif petdeeadlt apllmdspqc vlfgtfmiwf asitinlgpt flsgalaant
      181 esghnapscp lvhgpfrhyi lnvlwiainm icvaltlihl rklhkdltka nvearsgaae
      241 ehrkmrnylk rmeregvqrv kmflvitaay gifwgplffv tlvdhpsign ptgyevtlhi
      301 ayvhafvnpv lflslhrglr qglsesfcgc ceviaswilg psmpieniqp pryqepidav
      361 psppepplpp attcpdltdv allkpplppt gkhllddtvi vppdpwtlvs rskstssvyn
      421 edvsrrssil ipppltmndt ksmkrffvyn sesrksvhgs qv
//
LOCUS       XP_034190446             430 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608856 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190446
VERSION     XP_034190446.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334555.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="uncharacterized protein LOC117608856 isoform X4"
                     /calculated_mol_wt=46477
     Region          35..345
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          35..60
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410626"
     Region          67..91
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(90..91,101..106,108..109,112,163,165..169,199,
                     202..204,206..208,210..211,307,310..311,313..314,317,
                     323..324,326..328,331,334..335)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          150..169
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410626"
     Region          199..242
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          287..317
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     Region          324..345
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..430
                     /gene="LOC117608856"
                     /coded_by="XM_034334555.2:3..1295"
                     /db_xref="GeneID:117608856"
ORIGIN      
        1 mamiganlrf gigasgnvss natkvfqchp ggateatlif slgalgmgan fllmaliltk
       61 rqlrrwsqgl lfhqamvdca raaillplgs silncqpmtk cslvetafll lvtvstvnml
      121 ttvlndspif petdeeadlt apllmdspqc vlfgtfmiwf asitinlgpt flsgalaant
      181 esghnapscp lvhgpfrhyi lnvlwiainm icvaltlihl rklhkdltka nveavkvall
      241 tilvnttgnq qtnavsenst sangtpkrsg aaeehrkmrn ylkrmeregv qrvkmflvit
      301 aaygifwgpl ffvtlvdhps ignptgyevt lhiayvhafv npvlflslhr glrqglsesf
      361 cgcceviasw ilgpsmpien iqppryqepi davpsppepp lppattcpdl tdvallkppl
      421 pptgkhllln
//
LOCUS       XP_034190447             487 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608857 [Osmia lignaria lignaria].
ACCESSION   XP_034190447
VERSION     XP_034190447.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334556.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190447.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..487
                     /product="uncharacterized protein LOC117608857"
                     /calculated_mol_wt=56694
     Region          <35..402
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..487
                     /gene="LOC117608857"
                     /coded_by="XM_034334556.2:1..1464"
                     /db_xref="GeneID:117608857"
ORIGIN      
        1 mqrakrkkvk gkgkgkgkgk ktkkvkkrcl neretlsyeq eilennrqla rlrsrneele
       61 eeaevaegkl rqleedrsdv iahlkrnlee kiqeakelne rltaleelrk delaaykkke
      121 eameleyrnm enmlsaevkl aagklnaled wrlarldlmv kyekqeeema eqeerhkeal
      181 yeaeksvvig katmqkemnk rlqilseelr ksttlrmaei mhrairenva lnreldnmlk
      241 inkelesvst errkteetlr lksqlfeaes kitlkkvlkq rdvihkiand fqdlliryte
      301 aekyntrfag lqkilddiie gtnekkdkqi qalkkdiama mnyrqhllae iqekekqves
      361 lkgilnrvks lldealqvte dainkqqcid cigekllhsl ydilqtenli nvieytpsei
      421 enidcqyrtg ylgfveplkp ckdcgkqete sevedseeki sledleqnvp scllsvisss
      481 itshkee
//
LOCUS       XP_034190448             473 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside diphosphate phosphatase ENTPD5 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190448
VERSION     XP_034190448.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334557.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="nucleoside diphosphate phosphatase ENTPD5
                     isoform X1"
                     /calculated_mol_wt=52905
     Region          81..469
                     /region_name="ASKHA_NBD_NTPDase5-like"
                     /note="nucleotide-binding domain (NBD) of the
                     ectonucleoside triphosphate diphosphohydrolase 5
                     (NTPDase5)-like subfamily; cd24046"
                     /db_xref="CDD:466896"
     CDS             1..473
                     /gene="LOC117608858"
                     /coded_by="XM_034334557.2:113..1534"
                     /db_xref="GeneID:117608858"
ORIGIN      
        1 mcfnnqqfsy lrtenedife aeskprfkkg qpgkryfvvv allgilflgy iaiaydlkpv
       61 rlgskaidtl afsfnlqkpf yvvvidagst gsralafsfh esilggnlvl deelytetkp
      121 glsayserpk daakslivll dkvrsviprs ewqhtpltmr ataglrllpg hkaqeilqec
      181 rkmfeesgfl lsknsisime grdegifswf tvnflserfn thnpgstvaa ldlgggstqv
      241 tfspnpqqek kldghiysvn ifnhnmsvyt hsylgmglma arkeiltygm nldnvnpktv
      301 iqsecinpiv stewsyggvn yiikgpvngt hklvktqnfa ggeedrpivn fpeclkiiek
      361 yvgriknkpe glkdheiyaf syyfdratev glvdpffgga iqvsaflkha keacdypntd
      421 qpficldltf iyillrdgfg lepttklyly kkinghelsw algaafniiq ngl
//
LOCUS       XP_034190450             470 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside diphosphate phosphatase ENTPD5 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190450
VERSION     XP_034190450.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334559.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="nucleoside diphosphate phosphatase ENTPD5
                     isoform X2"
                     /calculated_mol_wt=52579
     Region          78..466
                     /region_name="ASKHA_NBD_NTPDase5-like"
                     /note="nucleotide-binding domain (NBD) of the
                     ectonucleoside triphosphate diphosphohydrolase 5
                     (NTPDase5)-like subfamily; cd24046"
                     /db_xref="CDD:466896"
     CDS             1..470
                     /gene="LOC117608858"
                     /coded_by="XM_034334559.2:160..1572"
                     /db_xref="GeneID:117608858"
ORIGIN      
        1 mevrhrklrt enedifeaes kprfkkgqpg kryfvvvall gilflgyiai aydlkpvrlg
       61 skaidtlafs fnlqkpfyvv vidagstgsr alafsfhesi lggnlvldee lytetkpgls
      121 ayserpkdaa kslivlldkv rsviprsewq htpltmrata glrllpghka qeilqecrkm
      181 feesgfllsk nsisimegrd egifswftvn flserfnthn pgstvaaldl gggstqvtfs
      241 pnpqqekkld ghiysvnifn hnmsvythsy lgmglmaark eiltygmnld nvnpktviqs
      301 ecinpivste wsyggvnyii kgpvngthkl vktqnfagge edrpivnfpe clkiiekyvg
      361 riknkpeglk dheiyafsyy fdratevglv dpffggaiqv saflkhakea cdypntdqpf
      421 icldltfiyi llrdgfglep ttklylykki nghelswalg aafniiqngl
//
LOCUS       XP_034190452             470 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside diphosphate phosphatase ENTPD5 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190452
VERSION     XP_034190452.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334561.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="nucleoside diphosphate phosphatase ENTPD5
                     isoform X2"
                     /calculated_mol_wt=52579
     Region          78..466
                     /region_name="ASKHA_NBD_NTPDase5-like"
                     /note="nucleotide-binding domain (NBD) of the
                     ectonucleoside triphosphate diphosphohydrolase 5
                     (NTPDase5)-like subfamily; cd24046"
                     /db_xref="CDD:466896"
     CDS             1..470
                     /gene="LOC117608858"
                     /coded_by="XM_034334561.2:169..1581"
                     /db_xref="GeneID:117608858"
ORIGIN      
        1 mevrhrklrt enedifeaes kprfkkgqpg kryfvvvall gilflgyiai aydlkpvrlg
       61 skaidtlafs fnlqkpfyvv vidagstgsr alafsfhesi lggnlvldee lytetkpgls
      121 ayserpkdaa kslivlldkv rsviprsewq htpltmrata glrllpghka qeilqecrkm
      181 feesgfllsk nsisimegrd egifswftvn flserfnthn pgstvaaldl gggstqvtfs
      241 pnpqqekkld ghiysvnifn hnmsvythsy lgmglmaark eiltygmnld nvnpktviqs
      301 ecinpivste wsyggvnyii kgpvngthkl vktqnfagge edrpivnfpe clkiiekyvg
      361 riknkpeglk dheiyafsyy fdratevglv dpffggaiqv saflkhakea cdypntdqpf
      421 icldltfiyi llrdgfglep ttklylykki nghelswalg aafniiqngl
//
LOCUS       XP_076545950             470 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside diphosphate phosphatase ENTPD5 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545950
VERSION     XP_076545950.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689835.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="nucleoside diphosphate phosphatase ENTPD5
                     isoform X2"
                     /calculated_mol_wt=52579
     Region          78..466
                     /region_name="ASKHA_NBD_NTPDase5-like"
                     /note="nucleotide-binding domain (NBD) of the
                     ectonucleoside triphosphate diphosphohydrolase 5
                     (NTPDase5)-like subfamily; cd24046"
                     /db_xref="CDD:466896"
     CDS             1..470
                     /gene="LOC117608858"
                     /coded_by="XM_076689835.1:280..1692"
                     /db_xref="GeneID:117608858"
ORIGIN      
        1 mevrhrklrt enedifeaes kprfkkgqpg kryfvvvall gilflgyiai aydlkpvrlg
       61 skaidtlafs fnlqkpfyvv vidagstgsr alafsfhesi lggnlvldee lytetkpgls
      121 ayserpkdaa kslivlldkv rsviprsewq htpltmrata glrllpghka qeilqecrkm
      181 feesgfllsk nsisimegrd egifswftvn flserfnthn pgstvaaldl gggstqvtfs
      241 pnpqqekkld ghiysvnifn hnmsvythsy lgmglmaark eiltygmnld nvnpktviqs
      301 ecinpivste wsyggvnyii kgpvngthkl vktqnfagge edrpivnfpe clkiiekyvg
      361 riknkpeglk dheiyafsyy fdratevglv dpffggaiqv saflkhakea cdypntdqpf
      421 icldltfiyi llrdgfglep ttklylykki nghelswalg aafniiqngl
//
LOCUS       XP_034190453             468 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside diphosphate phosphatase ENTPD5 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190453
VERSION     XP_034190453.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334562.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..468
                     /product="nucleoside diphosphate phosphatase ENTPD5
                     isoform X3"
                     /calculated_mol_wt=52274
     Region          76..464
                     /region_name="ASKHA_NBD_NTPDase5-like"
                     /note="nucleotide-binding domain (NBD) of the
                     ectonucleoside triphosphate diphosphohydrolase 5
                     (NTPDase5)-like subfamily; cd24046"
                     /db_xref="CDD:466896"
     CDS             1..468
                     /gene="LOC117608858"
                     /coded_by="XM_034334562.2:144..1550"
                     /db_xref="GeneID:117608858"
ORIGIN      
        1 mqyiplrten edifeaeskp rfkkgqpgkr yfvvvallgi lflgyiaiay dlkpvrlgsk
       61 aidtlafsfn lqkpfyvvvi dagstgsral afsfhesilg gnlvldeely tetkpglsay
      121 serpkdaaks livlldkvrs viprsewqht pltmratagl rllpghkaqe ilqecrkmfe
      181 esgfllskns isimegrdeg ifswftvnfl serfnthnpg stvaaldlgg gstqvtfspn
      241 pqqekkldgh iysvnifnhn msvythsylg mglmaarkei ltygmnldnv npktviqsec
      301 inpivstews yggvnyiikg pvngthklvk tqnfaggeed rpivnfpecl kiiekyvgri
      361 knkpeglkdh eiyafsyyfd ratevglvdp ffggaiqvsa flkhakeacd ypntdqpfic
      421 ldltfiyill rdgfgleptt klylykking helswalgaa fniiqngl
//
LOCUS       XP_034190466             303 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190466
VERSION     XP_034190466.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334575.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X1"
                     /calculated_mol_wt=34013
     Region          23..110
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          115..205
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          212..301
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..303
                     /gene="LOC117608865"
                     /coded_by="XM_034334575.2:189..1100"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mahqeiihhq psllkeaaiq igaggfagfv easimhpmdl iktrfqlqvk lkqydavyyt
       61 gigdcmrkmy kneglaafwk giippvvmet pkravkffsy eqykkllsnn tseqmvhfca
      121 gyltgvtegi lvnpfevvkv qmqsnrkrin espstfavtr hiiskqgfgl nglnkglsat
      181 vmrnsvfnaf yfgiyhsivp klqkhteyvs efftkftigf isgtvascmn ipfdvaksri
      241 qgpqgdtlyk gtlqtiylvy hregfralyk gllpkvlrlg pggaimlivy ekikvyftak
      301 fgd
//
LOCUS       XP_034190467             303 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190467
VERSION     XP_034190467.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334576.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X1"
                     /calculated_mol_wt=34013
     Region          23..110
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          115..205
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          212..301
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..303
                     /gene="LOC117608865"
                     /coded_by="XM_034334576.2:247..1158"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mahqeiihhq psllkeaaiq igaggfagfv easimhpmdl iktrfqlqvk lkqydavyyt
       61 gigdcmrkmy kneglaafwk giippvvmet pkravkffsy eqykkllsnn tseqmvhfca
      121 gyltgvtegi lvnpfevvkv qmqsnrkrin espstfavtr hiiskqgfgl nglnkglsat
      181 vmrnsvfnaf yfgiyhsivp klqkhteyvs efftkftigf isgtvascmn ipfdvaksri
      241 qgpqgdtlyk gtlqtiylvy hregfralyk gllpkvlrlg pggaimlivy ekikvyftak
      301 fgd
//
LOCUS       XP_034190469             303 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190469
VERSION     XP_034190469.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334578.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X1"
                     /calculated_mol_wt=34013
     Region          23..110
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          115..205
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          212..301
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..303
                     /gene="LOC117608865"
                     /coded_by="XM_034334578.1:236..1147"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mahqeiihhq psllkeaaiq igaggfagfv easimhpmdl iktrfqlqvk lkqydavyyt
       61 gigdcmrkmy kneglaafwk giippvvmet pkravkffsy eqykkllsnn tseqmvhfca
      121 gyltgvtegi lvnpfevvkv qmqsnrkrin espstfavtr hiiskqgfgl nglnkglsat
      181 vmrnsvfnaf yfgiyhsivp klqkhteyvs efftkftigf isgtvascmn ipfdvaksri
      241 qgpqgdtlyk gtlqtiylvy hregfralyk gllpkvlrlg pggaimlivy ekikvyftak
      301 fgd
//
LOCUS       XP_034190470             303 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190470
VERSION     XP_034190470.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334579.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X1"
                     /calculated_mol_wt=34013
     Region          23..110
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          115..205
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          212..301
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..303
                     /gene="LOC117608865"
                     /coded_by="XM_034334579.2:247..1158"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mahqeiihhq psllkeaaiq igaggfagfv easimhpmdl iktrfqlqvk lkqydavyyt
       61 gigdcmrkmy kneglaafwk giippvvmet pkravkffsy eqykkllsnn tseqmvhfca
      121 gyltgvtegi lvnpfevvkv qmqsnrkrin espstfavtr hiiskqgfgl nglnkglsat
      181 vmrnsvfnaf yfgiyhsivp klqkhteyvs efftkftigf isgtvascmn ipfdvaksri
      241 qgpqgdtlyk gtlqtiylvy hregfralyk gllpkvlrlg pggaimlivy ekikvyftak
      301 fgd
//
LOCUS       XP_034190471             303 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190471
VERSION     XP_034190471.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334580.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X1"
                     /calculated_mol_wt=34013
     Region          23..110
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          115..205
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          212..301
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..303
                     /gene="LOC117608865"
                     /coded_by="XM_034334580.1:251..1162"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mahqeiihhq psllkeaaiq igaggfagfv easimhpmdl iktrfqlqvk lkqydavyyt
       61 gigdcmrkmy kneglaafwk giippvvmet pkravkffsy eqykkllsnn tseqmvhfca
      121 gyltgvtegi lvnpfevvkv qmqsnrkrin espstfavtr hiiskqgfgl nglnkglsat
      181 vmrnsvfnaf yfgiyhsivp klqkhteyvs efftkftigf isgtvascmn ipfdvaksri
      241 qgpqgdtlyk gtlqtiylvy hregfralyk gllpkvlrlg pggaimlivy ekikvyftak
      301 fgd
//
LOCUS       XP_034190472             303 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190472
VERSION     XP_034190472.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334581.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X1"
                     /calculated_mol_wt=34013
     Region          23..110
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          115..205
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          212..301
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..303
                     /gene="LOC117608865"
                     /coded_by="XM_034334581.2:242..1153"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mahqeiihhq psllkeaaiq igaggfagfv easimhpmdl iktrfqlqvk lkqydavyyt
       61 gigdcmrkmy kneglaafwk giippvvmet pkravkffsy eqykkllsnn tseqmvhfca
      121 gyltgvtegi lvnpfevvkv qmqsnrkrin espstfavtr hiiskqgfgl nglnkglsat
      181 vmrnsvfnaf yfgiyhsivp klqkhteyvs efftkftigf isgtvascmn ipfdvaksri
      241 qgpqgdtlyk gtlqtiylvy hregfralyk gllpkvlrlg pggaimlivy ekikvyftak
      301 fgd
//
LOCUS       XP_076545951             238 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545951
VERSION     XP_076545951.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689836.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..238
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X2"
                     /calculated_mol_wt=26801
     Region          1..229
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     Region          147..233
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..238
                     /gene="LOC117608865"
                     /coded_by="XM_076689836.1:187..903"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mrkmyknegl aafwkgiipp vvmetpkrav kffsyeqykk llsnntseqm vhfcagyltg
       61 vtegilvnpf evvkvqmqsn rkrinespst favtrhiisk qgfglnglnk glsatvmrns
      121 vfnafyfgiy hsivpklqkh teyvsefftk ftigfisgtv ascmnipfdv aksriqgpqg
      181 dtlykgtlqt iylvyhregf ralykgllpk vlrlgpggai mlivyekikv yftakfgd
//
LOCUS       XP_034190473             238 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial 2-oxodicarboxylate carrier isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190473
VERSION     XP_034190473.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334582.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..238
                     /product="mitochondrial 2-oxodicarboxylate carrier isoform
                     X2"
                     /calculated_mol_wt=26801
     Region          1..229
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     Region          147..233
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..238
                     /gene="LOC117608865"
                     /coded_by="XM_034334582.1:149..865"
                     /db_xref="GeneID:117608865"
ORIGIN      
        1 mrkmyknegl aafwkgiipp vvmetpkrav kffsyeqykk llsnntseqm vhfcagyltg
       61 vtegilvnpf evvkvqmqsn rkrinespst favtrhiisk qgfglnglnk glsatvmrns
      121 vfnafyfgiy hsivpklqkh teyvsefftk ftigfisgtv ascmnipfdv aksriqgpqg
      181 dtlykgtlqt iylvyhregf ralykgllpk vlrlgpggai mlivyekikv yftakfgd
//
LOCUS       XP_034190488             207 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608871 [Osmia lignaria lignaria].
ACCESSION   XP_034190488
VERSION     XP_034190488.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334597.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..207
                     /product="uncharacterized protein LOC117608871"
                     /calculated_mol_wt=23665
     CDS             1..207
                     /gene="LOC117608871"
                     /coded_by="XM_034334597.2:351..974"
                     /db_xref="GeneID:117608871"
ORIGIN      
        1 mphkksqgrg kkikekekek keiknddqyf ediididenv tkdfnflvna pvseddhfvf
       61 esekswdvdi skyseyftld lntlaaaies ipfnenvdid tkyftndqlt niynnaelgk
      121 rrynkilsnl etpitnnpkn eesqdsveds aeeldfllsl qepvnepsni lkslpgaynt
      181 dsklvskpgt stkpmdlekw ldsvldd
//
LOCUS       XP_034190489             202 aa            linear   INV 03-SEP-2025
DEFINITION  DNA replication complex GINS protein PSF3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190489
VERSION     XP_034190489.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334598.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="DNA replication complex GINS protein PSF3"
                     /calculated_mol_wt=23134
     Region          11..76
                     /region_name="GINS_B_Psf3"
                     /note="beta-strand (B) domain of GINS complex protein
                     Psf3; cd21693"
                     /db_xref="CDD:412029"
     Site            order(11..12,14,16..18,20..21,32..35,58..59,62..63,70)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:412029"
     Region          70..177
                     /region_name="GINS_A_psf3"
                     /note="Alpha-helical domain of GINS complex protein Psf3
                     (partner of Sld5 3); cd11713"
                     /db_xref="CDD:212551"
     Site            order(105,108,129,133,137..138,153,156,158,160..170)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:212551"
     CDS             1..202
                     /gene="Psf3"
                     /coded_by="XM_034334598.2:35..643"
                     /db_xref="GeneID:117608872"
ORIGIN      
        1 malsqsylpn yfaindilct eerltcttev elprlgfldc ssqtanlkig skvefplwla
       61 lslknlrnpi vsvdipkiyk egyreilkad adavvlskwn pffyelgmhv rklndrsseq
      121 itdsllqtfk srlrlvmdwa qnpisdptlg kqlprlerel flsgrkakvr lsewlqkgtn
      181 silpsevtan lkkrkrtnye ld
//
LOCUS       XP_076545952             369 aa            linear   INV 03-SEP-2025
DEFINITION  uridine phosphorylase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545952
VERSION     XP_076545952.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689837.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="uridine phosphorylase 1 isoform X1"
                     /calculated_mol_wt=41771
     Region          60..332
                     /region_name="UP_hUPP-like"
                     /note="uridine phosphorylases similar to a human UPP1 and
                     UPP2; cd17763"
                     /db_xref="CDD:350163"
     Site            order(63..64,88,123..124,139,142..143,145..146,149..150,
                     152..153,190..191,198..202,207,240..245,247,249..256,258,
                     260,264,278,286,289..290)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350163"
     Site            order(87..89,92,123,139,167..172,242,246,248,276..279,302,
                     310)
                     /site_type="active"
                     /db_xref="CDD:350163"
     Site            order(87..89,92,123,167..170)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:350163"
     CDS             1..369
                     /gene="LOC117608815"
                     /coded_by="XM_076689837.1:241..1350"
                     /db_xref="GeneID:117608815"
ORIGIN      
        1 mptctcekrm fneenhvcqm kppveageye eetqeydpav rytdgsvrlr npnielmdqd
       61 ilyhlalgsg shdlvemfgd vkfvcmggtp krmeqfahyi mkeighklpa gttlldisqy
      121 syrysmykvg pvlsishgmg ipsvgillhe viklmyhakv kdpiffrigt cggigieggt
      181 vviseeavdg mlkpyldlpv lgkivrrpsk ldrqlahdlk alahrddpyd tvigktmcty
      241 dfyegqgrmd gafceftend kmeylnklhk agvvniemes lsfaalthha giksavvcvt
      301 lidrfkgdqv lapkevldew qmrpqqlisr yitrylqrkg rlsleghgsm fvksprrfkl
      361 vqqesenyd
//
LOCUS       XP_076545953             341 aa            linear   INV 03-SEP-2025
DEFINITION  uridine phosphorylase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545953
VERSION     XP_076545953.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689838.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uridine phosphorylase 1 isoform X2"
                     /calculated_mol_wt=38470
     Region          32..304
                     /region_name="UP_hUPP-like"
                     /note="uridine phosphorylases similar to a human UPP1 and
                     UPP2; cd17763"
                     /db_xref="CDD:350163"
     Site            order(35..36,60,95..96,111,114..115,117..118,121..122,
                     124..125,162..163,170..174,179,212..217,219,221..228,230,
                     232,236,250,258,261..262)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350163"
     Site            order(59..61,64,95,111,139..144,214,218,220,248..251,274,
                     282)
                     /site_type="active"
                     /db_xref="CDD:350163"
     Site            order(59..61,64,95,139..142)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:350163"
     CDS             1..341
                     /gene="LOC117608815"
                     /coded_by="XM_076689838.1:178..1203"
                     /db_xref="GeneID:117608815"
ORIGIN      
        1 msllledeel ldeytdgsvr lrnpnielmd qdilyhlalg sgshdlvemf gdvkfvcmgg
       61 tpkrmeqfah yimkeighkl pagttlldis qysyrysmyk vgpvlsishg mgipsvgill
      121 heviklmyha kvkdpiffri gtcggigieg gtvviseeav dgmlkpyldl pvlgkivrrp
      181 skldrqlahd lkalahrddp ydtvigktmc tydfyegqgr mdgafcefte ndkmeylnkl
      241 hkagvvniem eslsfaalth hagiksavvc vtlidrfkgd qvlapkevld ewqmrpqqli
      301 sryitrylqr kgrlsleghg smfvksprrf klvqqeseny d
//
LOCUS       XP_076545954             313 aa            linear   INV 03-SEP-2025
DEFINITION  uridine phosphorylase 2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545954
VERSION     XP_076545954.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689839.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..313
                     /product="uridine phosphorylase 2 isoform X3"
                     /calculated_mol_wt=35210
     Region          4..276
                     /region_name="UP_hUPP-like"
                     /note="uridine phosphorylases similar to a human UPP1 and
                     UPP2; cd17763"
                     /db_xref="CDD:350163"
     Site            order(7..8,32,67..68,83,86..87,89..90,93..94,96..97,
                     134..135,142..146,151,184..189,191,193..200,202,204,208,
                     222,230,233..234)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:350163"
     Site            order(31..33,36,67,83,111..116,186,190,192,220..223,246,
                     254)
                     /site_type="active"
                     /db_xref="CDD:350163"
     Site            order(31..33,36,67,111..114)
                     /site_type="other"
                     /note="phosphate binding site [ion binding]"
                     /db_xref="CDD:350163"
     CDS             1..313
                     /gene="LOC117608815"
                     /coded_by="XM_076689839.1:181..1122"
                     /db_xref="GeneID:117608815"
ORIGIN      
        1 mdqdilyhla lgsgshdlve mfgdvkfvcm ggtpkrmeqf ahyimkeigh klpagttlld
       61 isqysyrysm ykvgpvlsis hgmgipsvgi llheviklmy hakvkdpiff rigtcggigi
      121 eggtvvisee avdgmlkpyl dlpvlgkivr rpskldrqla hdlkalahrd dpydtvigkt
      181 mctydfyegq grmdgafcef tendkmeyln klhkagvvni emeslsfaal thhagiksav
      241 vcvtlidrfk gdqvlapkev ldewqmrpqq lisryitryl qrkgrlsleg hgsmfvkspr
      301 rfklvqqese nyd
//
LOCUS       XP_034194912            2134 aa            linear   INV 03-SEP-2025
DEFINITION  U5 small nuclear ribonucleoprotein l(3)72Ab [Osmia lignaria
            lignaria].
ACCESSION   XP_034194912
VERSION     XP_034194912.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194912.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2134
                     /product="U5 small nuclear ribonucleoprotein l(3)72Ab"
                     /calculated_mol_wt=242325
     Region          256..361
                     /region_name="Helicase_PWI"
                     /note="N-terminal helicase PWI domain; pfam18149"
                     /db_xref="CDD:436309"
     Region          458..671
                     /region_name="DEXHc_Brr2_1"
                     /note="N-terminal DEXH-box helicase domain of spliceosomal
                     Brr2 RNA helicase; cd18019"
                     /db_xref="CDD:350777"
     Region          470..1014
                     /region_name="BRR2"
                     /note="Replicative superfamily II helicase [Replication,
                     recombination and repair]; COG1204"
                     /db_xref="CDD:440817"
     Site            order(474,476,479,500..504,609..610)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350777"
     Site            order(538..540,566..570,572..573,583,585..586,589,593,618)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350777"
     Region          975..1279
                     /region_name="Sec63"
                     /note="Sec63 Brl domain; pfam02889"
                     /db_xref="CDD:460740"
     Region          1290..1845
                     /region_name="BRR2"
                     /note="Replicative superfamily II helicase [Replication,
                     recombination and repair]; COG1204"
                     /db_xref="CDD:440817"
     Region          1319..1509
                     /region_name="DEXHc_Brr2_2"
                     /note="C-terminal D[D/E]X[H/Q]-box helicase domain of
                     spliceosomal Brr2 RNA helicase; cd18021"
                     /db_xref="CDD:350779"
     Site            order(1319,1321..1323,1326,1345..1351)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350779"
     Region          1806..2119
                     /region_name="SEC63"
                     /note="Domain of unknown function in Sec63p, Brr2p and
                     other proteins; smart00611"
                     /db_xref="CDD:214744"
     CDS             1..2134
                     /gene="l(3)72Ab"
                     /coded_by="XM_034339021.2:176..6580"
                     /db_xref="GeneID:117611112"
ORIGIN      
        1 madaaarqlq yeykansnlv lqadvrlier rsrdeatgev mslvgkldgt rmgdraqrtk
       61 pgkaeerkvk rqkrdeaqyd farmkgatll segvdemvgi vyrpktqetr qtyevllsfi
      121 qealgdqprd ilcgaadevl avlkndrlke kekkketell lgtlaeerfa llvnlgkkit
      181 dfgsdekgtt neenidetyg invqfeesse eddedvygev renddegdeg eeanddraih
      241 aenlggaeem kkekplhpld idaywlqrrl sriyddamis qaraaevlav lkdagddrdc
      301 enqlvlllgy dcfdfikqlk kyrhtiayct mlassqsese rqkirnkmnd dptlakilrq
      361 ldtgkgddda detmearaqr krreenedtg gpggqvqgtr nlidledlvf aqgshfmank
      421 rcqlpdgsfr kqrkgyeevh vpalkpkpfa eneklhpidq lpkyvqpafe gfktlnriqs
      481 rlhqaalesd enlllcaptg agktnvallc mmreigkhin adgtinadef kiiyvapmrs
      541 lvqemvgnfs krlstynltv seltgdhqlt reqiaatqvi vctpekwdii trkggektft
      601 slvrliiide ihllhdergp vlealvarti rniettqedv rliglsatlp nyqdvaaflr
      661 ikpetglfyf dnsfrpvale qqyigvtekk alkrfqvmne ivyektmeha grnqvlvfvh
      721 srketgktar airdmclekd tlgqflregs asmevlrtea eqvknqelkd llpygfaihh
      781 agmtrvdrtl vedlfadrhi qvlvstatla wgvnlpahtv iikgtqvynp ekgrwvelsa
      841 ldvlqmlgra grpqydtkge gilitnhsel qyylsllnqq lpiesqlisk msdmlnaeiv
      901 lgtiqnirda vtwlgytyly irmlrcpnly sighdklked pllelhradl ihsaavgldr
      961 sglikydrks gnfqatelgr iashyycthd tmsiynqllk rtlseielfr vfslssefkh
     1021 invreeekle lqklmervpi pvkesieeps akvnvllqay isqlklegfa lmsdmvfvtq
     1081 sasrlmraif eivlfrgwaq ladkclslck midrrmwqsm splrqfrkmp eeivkkiekk
     1141 nfpwerlydl gpneigelir vpklgktihk yihqfpklel sthiqpitrs tlrveltitp
     1201 dfqwdekvhg aseafwilve dvdsevilhh eyfllkakya tdehlikffv pvfeplppqy
     1261 flrvvsnrwi gaetqlpvsf rhlilpeknl pptelldlqp lpitalrnsk feslyadkfp
     1321 qfnpiqtqvf navynsddnv fvgaptgsgk ttiaefavlr lltqnpegrc vymvskeala
     1381 eliyvdwtik fnqqlgrkvv lltgetgtdl kllakgqiii ttadkwdvls rrwkqrknvq
     1441 niqlfivdel qliggeegpv levacsrary issqldkptr iialsaslad akdaaqwlga
     1501 paaatfnfhp svrpvplelh vqginithna srlaamakpv ynavlryash kpviafvptr
     1561 rqarltaidl ltftaaegqp srffhaeead ikpfldrmtd ktlketlsqg vaylheglsa
     1621 ddrrlveqlf dsgaiqiava trdlcwglsi tshlvivmdt qcyngkthay edypitdvlq
     1681 mvaranrple dddakcvllc qsskkdffkk flneplpves hldhrlhdhf naeivtktie
     1741 nkqdavdylt wtflyrrltq npnyyglqgv thrhlsdhls elvestlsdl eqakcvaved
     1801 emdtlplnlg miaayyyiny atielfslsl nnktkirgll eiisaaaeye tvpvrqreen
     1861 llrslaarlp hapqaprmad phvkaqlllq ahlsriqlgp elqkdtelvl skairliqac
     1921 vdvlsssgwl apavaamela qmvtqamwsk dsylkqlphf saetikrctd kgvetvfdvm
     1981 eledddrnrl lqlseaqmad vakfcnrypn iemsyevqdk dklrsggavn vivqlerede
     2041 vtgpvvapff pqkreegwwv vigdpktnsl lsikrltlqq kakvkldfva papgqhsytl
     2101 yfmsdaylgc dqeykftinv geyesdvssg sesd
//
LOCUS       XP_034194913            2134 aa            linear   INV 03-SEP-2025
DEFINITION  U5 small nuclear ribonucleoprotein l(3)72Ab [Osmia lignaria
            lignaria].
ACCESSION   XP_034194913
VERSION     XP_034194913.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339022.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194913.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2134
                     /product="U5 small nuclear ribonucleoprotein l(3)72Ab"
                     /calculated_mol_wt=242325
     Region          256..361
                     /region_name="Helicase_PWI"
                     /note="N-terminal helicase PWI domain; pfam18149"
                     /db_xref="CDD:436309"
     Region          458..671
                     /region_name="DEXHc_Brr2_1"
                     /note="N-terminal DEXH-box helicase domain of spliceosomal
                     Brr2 RNA helicase; cd18019"
                     /db_xref="CDD:350777"
     Region          470..1014
                     /region_name="BRR2"
                     /note="Replicative superfamily II helicase [Replication,
                     recombination and repair]; COG1204"
                     /db_xref="CDD:440817"
     Site            order(474,476,479,500..504,609..610)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350777"
     Site            order(538..540,566..570,572..573,583,585..586,589,593,618)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350777"
     Region          975..1279
                     /region_name="Sec63"
                     /note="Sec63 Brl domain; pfam02889"
                     /db_xref="CDD:460740"
     Region          1290..1845
                     /region_name="BRR2"
                     /note="Replicative superfamily II helicase [Replication,
                     recombination and repair]; COG1204"
                     /db_xref="CDD:440817"
     Region          1319..1509
                     /region_name="DEXHc_Brr2_2"
                     /note="C-terminal D[D/E]X[H/Q]-box helicase domain of
                     spliceosomal Brr2 RNA helicase; cd18021"
                     /db_xref="CDD:350779"
     Site            order(1319,1321..1323,1326,1345..1351)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350779"
     Region          1806..2119
                     /region_name="SEC63"
                     /note="Domain of unknown function in Sec63p, Brr2p and
                     other proteins; smart00611"
                     /db_xref="CDD:214744"
     CDS             1..2134
                     /gene="l(3)72Ab"
                     /coded_by="XM_034339022.2:151..6555"
                     /db_xref="GeneID:117611112"
ORIGIN      
        1 madaaarqlq yeykansnlv lqadvrlier rsrdeatgev mslvgkldgt rmgdraqrtk
       61 pgkaeerkvk rqkrdeaqyd farmkgatll segvdemvgi vyrpktqetr qtyevllsfi
      121 qealgdqprd ilcgaadevl avlkndrlke kekkketell lgtlaeerfa llvnlgkkit
      181 dfgsdekgtt neenidetyg invqfeesse eddedvygev renddegdeg eeanddraih
      241 aenlggaeem kkekplhpld idaywlqrrl sriyddamis qaraaevlav lkdagddrdc
      301 enqlvlllgy dcfdfikqlk kyrhtiayct mlassqsese rqkirnkmnd dptlakilrq
      361 ldtgkgddda detmearaqr krreenedtg gpggqvqgtr nlidledlvf aqgshfmank
      421 rcqlpdgsfr kqrkgyeevh vpalkpkpfa eneklhpidq lpkyvqpafe gfktlnriqs
      481 rlhqaalesd enlllcaptg agktnvallc mmreigkhin adgtinadef kiiyvapmrs
      541 lvqemvgnfs krlstynltv seltgdhqlt reqiaatqvi vctpekwdii trkggektft
      601 slvrliiide ihllhdergp vlealvarti rniettqedv rliglsatlp nyqdvaaflr
      661 ikpetglfyf dnsfrpvale qqyigvtekk alkrfqvmne ivyektmeha grnqvlvfvh
      721 srketgktar airdmclekd tlgqflregs asmevlrtea eqvknqelkd llpygfaihh
      781 agmtrvdrtl vedlfadrhi qvlvstatla wgvnlpahtv iikgtqvynp ekgrwvelsa
      841 ldvlqmlgra grpqydtkge gilitnhsel qyylsllnqq lpiesqlisk msdmlnaeiv
      901 lgtiqnirda vtwlgytyly irmlrcpnly sighdklked pllelhradl ihsaavgldr
      961 sglikydrks gnfqatelgr iashyycthd tmsiynqllk rtlseielfr vfslssefkh
     1021 invreeekle lqklmervpi pvkesieeps akvnvllqay isqlklegfa lmsdmvfvtq
     1081 sasrlmraif eivlfrgwaq ladkclslck midrrmwqsm splrqfrkmp eeivkkiekk
     1141 nfpwerlydl gpneigelir vpklgktihk yihqfpklel sthiqpitrs tlrveltitp
     1201 dfqwdekvhg aseafwilve dvdsevilhh eyfllkakya tdehlikffv pvfeplppqy
     1261 flrvvsnrwi gaetqlpvsf rhlilpeknl pptelldlqp lpitalrnsk feslyadkfp
     1321 qfnpiqtqvf navynsddnv fvgaptgsgk ttiaefavlr lltqnpegrc vymvskeala
     1381 eliyvdwtik fnqqlgrkvv lltgetgtdl kllakgqiii ttadkwdvls rrwkqrknvq
     1441 niqlfivdel qliggeegpv levacsrary issqldkptr iialsaslad akdaaqwlga
     1501 paaatfnfhp svrpvplelh vqginithna srlaamakpv ynavlryash kpviafvptr
     1561 rqarltaidl ltftaaegqp srffhaeead ikpfldrmtd ktlketlsqg vaylheglsa
     1621 ddrrlveqlf dsgaiqiava trdlcwglsi tshlvivmdt qcyngkthay edypitdvlq
     1681 mvaranrple dddakcvllc qsskkdffkk flneplpves hldhrlhdhf naeivtktie
     1741 nkqdavdylt wtflyrrltq npnyyglqgv thrhlsdhls elvestlsdl eqakcvaved
     1801 emdtlplnlg miaayyyiny atielfslsl nnktkirgll eiisaaaeye tvpvrqreen
     1861 llrslaarlp hapqaprmad phvkaqlllq ahlsriqlgp elqkdtelvl skairliqac
     1921 vdvlsssgwl apavaamela qmvtqamwsk dsylkqlphf saetikrctd kgvetvfdvm
     1981 eledddrnrl lqlseaqmad vakfcnrypn iemsyevqdk dklrsggavn vivqlerede
     2041 vtgpvvapff pqkreegwwv vigdpktnsl lsikrltlqq kakvkldfva papgqhsytl
     2101 yfmsdaylgc dqeykftinv geyesdvssg sesd
//
LOCUS       XP_034194914             248 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: kinesin-like protein KIF11-A [Osmia lignaria
            lignaria].
ACCESSION   XP_034194914
VERSION     XP_034194914.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339023.2
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194914.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="LOW QUALITY PROTEIN: kinesin-like protein
                     KIF11-A"
                     /calculated_mol_wt=27295
     Region          <1..247
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     CDS             1..248
                     /gene="LOC117611114"
                     /coded_by="XM_034339023.2:91..837"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 1 codon"
                     /db_xref="GeneID:117611114"
ORIGIN      
        1 meeinsdrnl lcqsdssagv iprslshlfd kldlldytir vsflelyned lfdllssndd
       61 gskirlceda skkgaviihg leevtihnks evykilekes grrqtavtxm nsqssrsqtv
      121 fsitvlmken tsdgkevlkt gklnlinlag seyvgrssav gkkareagsv nqslltlgrv
      181 itvvverapy ilyreskltk llqeslddri kasiiaiisp aainldetls tldyayrakn
      241 itnrpkii
//
LOCUS       XP_034194915             237 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117611115 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194915
VERSION     XP_034194915.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339024.2
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194915.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117611115"
                     /calculated_mol_wt=26649
     Region          29..194
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..237
                     /gene="LOC117611115"
                     /coded_by="XM_034339024.2:1662..2375"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 1 codon"
                     /db_xref="GeneID:117611115"
ORIGIN      
        1 mevpteldfk niakdyyniw nfpsclgsid gkhikikclp hsgsxfynyk qffsivlqgv
       61 vdanykfmni dvggygkqsd ggtlrsstff lymsdgrlti pndtalpnse inapyvfigd
      121 evypllknll kpysgqqlna hkeynfnarl srarrtveca fgiifskwri lskaietsvt
      181 vaddivkaic vlhnaiidke gtehhlrnls farelsvrhd fdrptqvaqn istdprv
//
LOCUS       XP_076545956             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611116 [Osmia lignaria lignaria].
ACCESSION   XP_076545956
VERSION     XP_076545956.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689841.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC117611116"
                     /calculated_mol_wt=16810
     CDS             1..150
                     /gene="LOC117611116"
                     /coded_by="XM_076689841.1:1..453"
                     /db_xref="GeneID:117611116"
ORIGIN      
        1 mqalitldsq ksalsllkma sicaehfdkn clvkagllsl rlivkpnsvp mifphanner
       61 pemisthnyp aislqeqsse mpaltennsv ilssletpst srkrirypgd isenvldkmt
      121 pkrlkasvrq fresckkkdr rikclqsqqt
//
LOCUS       XP_076545957             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611116 [Osmia lignaria lignaria].
ACCESSION   XP_076545957
VERSION     XP_076545957.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689842.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC117611116"
                     /calculated_mol_wt=16810
     CDS             1..150
                     /gene="LOC117611116"
                     /coded_by="XM_076689842.1:1..453"
                     /db_xref="GeneID:117611116"
ORIGIN      
        1 mqalitldsq ksalsllkma sicaehfdkn clvkagllsl rlivkpnsvp mifphanner
       61 pemisthnyp aislqeqsse mpaltennsv ilssletpst srkrirypgd isenvldkmt
      121 pkrlkasvrq fresckkkdr rikclqsqqt
//
LOCUS       XP_076545958             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611116 [Osmia lignaria lignaria].
ACCESSION   XP_076545958
VERSION     XP_076545958.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689843.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC117611116"
                     /calculated_mol_wt=16810
     CDS             1..150
                     /gene="LOC117611116"
                     /coded_by="XM_076689843.1:1..453"
                     /db_xref="GeneID:117611116"
ORIGIN      
        1 mqalitldsq ksalsllkma sicaehfdkn clvkagllsl rlivkpnsvp mifphanner
       61 pemisthnyp aislqeqsse mpaltennsv ilssletpst srkrirypgd isenvldkmt
      121 pkrlkasvrq fresckkkdr rikclqsqqt
//
LOCUS       XP_076545959             150 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611116 [Osmia lignaria lignaria].
ACCESSION   XP_076545959
VERSION     XP_076545959.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689844.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..150
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..150
                     /product="uncharacterized protein LOC117611116"
                     /calculated_mol_wt=16810
     CDS             1..150
                     /gene="LOC117611116"
                     /coded_by="XM_076689844.1:1..453"
                     /db_xref="GeneID:117611116"
ORIGIN      
        1 mqalitldsq ksalsllkma sicaehfdkn clvkagllsl rlivkpnsvp mifphanner
       61 pemisthnyp aislqeqsse mpaltennsv ilssletpst srkrirypgd isenvldkmt
      121 pkrlkasvrq fresckkkdr rikclqsqqt
//
LOCUS       XP_076545960             133 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305619 [Osmia lignaria lignaria].
ACCESSION   XP_076545960
VERSION     XP_076545960.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689845.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..133
                     /product="uncharacterized protein LOC143305619"
                     /calculated_mol_wt=15251
     Region          <27..96
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..133
                     /gene="LOC143305619"
                     /coded_by="XM_076689845.1:247..648"
                     /db_xref="GeneID:143305619"
ORIGIN      
        1 mclilvpgah yilqmarsvq ieekyvvdey asehnktilr lppyhcelnp ielawasvkn
       61 yvksrnteyn ipqvkqllte giegvtpemw kncirhtigv eqklmhmdrf sqdtlempti
      121 dsdvdmsdtd vsd
//
LOCUS       XP_076545961             138 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305620 [Osmia
            lignaria lignaria].
ACCESSION   XP_076545961
VERSION     XP_076545961.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689846.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..138
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305620"
                     /calculated_mol_wt=16107
     CDS             1..138
                     /gene="LOC143305620"
                     /coded_by="XM_076689846.1:96..512"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:143305620"
ORIGIN      
        1 masrdrdrgr kyisgaeklk rkkqrdgyik qqtnnimkyv iatighsrqp nedeehlamy
       61 qipleegkqq lntpvkeeqe etnetemden enngqraivk liekktlkiw klkhkkkktg
      121 istlkilarg qlkehnkq
//
LOCUS       XP_034185000             533 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor IIFalpha [Osmia lignaria lignaria].
ACCESSION   XP_034185000
VERSION     XP_034185000.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329109.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="transcription factor IIFalpha"
                     /calculated_mol_wt=60397
     Region          14..529
                     /region_name="TFIIF_alpha"
                     /note="Transcription initiation factor IIF, alpha subunit
                     (TFIIF-alpha); pfam05793"
                     /db_xref="CDD:310411"
     CDS             1..533
                     /gene="TfIIFalpha"
                     /coded_by="XM_034329109.2:297..1898"
                     /db_xref="GeneID:117606546"
ORIGIN      
        1 mttpvapkvp vapsvqefsi rvpknnkkkh nvmrfnatln vdfskwsqvk merennmkey
       61 kgveeempkf gagsefgrda reearrkkfg itsrkykped qpwilksggk tgkkfkgire
      121 ggvsenaayy vfthasdgai eafplhewyn fqpiqrykal saeeaeqefs rrnkvmnyfs
      181 lmlrkrlrnd eegaddeemi eggkgkkpgk kekelkisem dewmdsdddd snsnddetkk
      241 nseeeedlkk kkkakansqk kkkknasdde afeesddgde egrecdyisd ssdseseleq
      301 qkeiksvvee dalrkllasd ededdeeqad kkdgdedkde ddenkekedk dkdktskdad
      361 kkkdkkkkkk lakkkdlkkl gsgkdsssdf ssdsgsdsda vkekdnkksn sahssrsatp
      421 tpagisdsmk rkqsgspdls qskklklens nlvpltayip gasesgised avrrylmrkp
      481 mtttellqkf kskktgltse qlvnvmtqil kkinptkqti knkmylsikt qsn
//
LOCUS       XP_034184999             528 aa            linear   INV 03-SEP-2025
DEFINITION  anosmin-1 Kallmann syndrome 1 [Osmia lignaria lignaria].
ACCESSION   XP_034184999
VERSION     XP_034184999.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329108.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184999.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..528
                     /product="anosmin-1 Kallmann syndrome 1"
                     /calculated_mol_wt=61199
     Region          101..146
                     /region_name="WAP"
                     /note="WAP-type (Whey Acidic Protein) 'four-disulfide
                     core'; pfam00095"
                     /db_xref="CDD:459672"
     Region          <153..>352
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     CDS             1..528
                     /gene="Kal1"
                     /coded_by="XM_034329108.2:1..1587"
                     /db_xref="GeneID:117606545"
ORIGIN      
        1 mgygrfrrcl pplltygsps pppmtlpvgs cytvksrvhr slpavlkkww lwwlllpgcl
       61 aswtkyieey dsirvarcda ycgsnypekc menclasnyt kpgycpdkas mslfeavclv
      121 actddsrcpd lakccrhdcg vtcmhplgld nrtdlppipe nlqirqqknn lvlltwhnsk
      181 gqclnsssld knirylveer hllgpryles rlsswtvrhv stkshatlre rlktghwyqf
      241 riaainengs rgysqpsrpf ktreprnpke pqnltlsdar lvggklritl rwvkpasdvp
      301 itfykvfwsr lvhgptndsi lvyhrtvlkd ktcyelknle lkcqyflqvq avalygsrrl
      361 tsrkaskvfn stdymayanv grrsrrcery qtglhvrrmt chceevkvrl vwptnrdvka
      421 ynvswreesc sssqenhaah rkkvswkvvq tphldiwrlr sectynvnvr nifddessdk
      481 hgtsieffat gcqkeydqke kkyskdkike cknkslrskr yftglsli
//
LOCUS       XP_034185005             394 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606549 [Osmia lignaria lignaria].
ACCESSION   XP_034185005
VERSION     XP_034185005.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329114.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..394
                     /product="uncharacterized protein LOC117606549"
                     /calculated_mol_wt=45170
     Region          <181..384
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..394
                     /gene="LOC117606549"
                     /coded_by="XM_034329114.2:503..1687"
                     /db_xref="GeneID:117606549"
ORIGIN      
        1 mnfltlsrln afiniisgnm lpvtskktnl pinlkiywvi vwmiqlsylt acilgihyvp
       61 tenslkdstv amavtveait lsvymnsrkn llrqlieqln etlkegdeml rtitmetvkp
      121 lqkvlkmyti gsigsvniwi llqfvkmtrk efyyvdyrvp avfsaepfsv nvflaggmlv
      181 cmgstyaiak kvsldlymih lvrlmtaqyk ylrirfamil rhnlknvedd vwqsvsweee
      241 qkvkqemrml tchyesiikm atilkkvlap nigvlyinnv frfsflsiml masatemeky
      301 limsysigal iqlymlcfsi qelfeasiav addafyekwc vydtsllhat aimtfgskle
      361 cklssvrsid ltlptfmsil nqaysvcllf lkar
//
LOCUS       XP_034185006             341 aa            linear   INV 03-SEP-2025
DEFINITION  TWiK family of potassium channels protein 7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185006
VERSION     XP_034185006.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329115.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="TWiK family of potassium channels protein 7"
                     /calculated_mol_wt=38313
     Region          <138..194
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          225..299
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     CDS             1..341
                     /gene="LOC117606550"
                     /coded_by="XM_034329115.2:153..1178"
                     /db_xref="GeneID:117606550"
ORIGIN      
        1 mtrfpirssl rsrdsnstst dpdprerikd ccrklvafmc tqvgvgglvv gyaiigafgf
       61 tsiesqaqip savcsrdsir kqyadelwls tasnqtvnif dkttfftvsn qilrryqdnf
      121 tgnygkencs glkpsdlwsf paammfclsv ftmigygtlv pqtpwgkavt viyavmgipl
      181 yvlyflnmgk vlaqtfrwly twlhectgkr kpgqritvps taclwvifgy vligsiifae
      241 wegwdyldsa yfcvtslcki gmgdlvpgwt hgdltadsqt kliinfvyvl lgmgliamcy
      301 dlmkedvyvk arelkeqfiq ivdaahyqle tccrshisdt k
//
LOCUS       XP_034185007             321 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase subunit gamma-2, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034185007
VERSION     XP_034185007.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329116.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="DNA polymerase subunit gamma-2, mitochondrial"
                     /calculated_mol_wt=37262
     Region          203..317
                     /region_name="HGTP_anticodon"
                     /note="HGTP anticodon binding domain, as found at the
                     C-terminus of histidyl, glycyl, threonyl and prolyl tRNA
                     synthetases, which are classified as a group of class II
                     aminoacyl-tRNA synthetases (aaRS). In aaRSs, the anticodon
                     binding domain is responsible for...; cl00266"
                     /db_xref="CDD:469699"
     Site            order(234..235,278,286,296,298)
                     /site_type="active"
                     /note="anticodon binding site [active]"
                     /db_xref="CDD:238379"
     CDS             1..321
                     /gene="DNApol-gamma35"
                     /coded_by="XM_034329116.2:1..966"
                     /db_xref="GeneID:117606551"
ORIGIN      
        1 mnitrvlkel sphfislteq glmygpqgkm ivrnleehwf lhcvtmspyn iflsdeysdt
       61 ldfitktats dipfglarvk sckdswnrdi ltrfklnlhk iaeiaifnnd tetkdlfhtv
      121 qkqrkiwwrr laqypsrfvl tevkkaknhd linieaqfpf gnitverith ymdiqklfpq
      181 idnkqvseqq miehsvsldw gclallcdty diskktkmhi hsklaphkia frikrpsnda
      241 nvqnndlnrf vlylnnmlkt kgmntiltts eqiidtclvp fivlvdetsl engiiyvmdr
      301 sttlsesvhi tdlvnhismr c
//
LOCUS       XP_076545963             298 aa            linear   INV 03-SEP-2025
DEFINITION  carbonic anhydrase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076545963
VERSION     XP_076545963.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689848.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="carbonic anhydrase 1 isoform X1"
                     /calculated_mol_wt=33424
     Region          25..264
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; smart01057"
                     /db_xref="CDD:215000"
     Site            order(82,108,110,112,116,129,213)
                     /site_type="active"
                     /db_xref="CDD:238200"
     Site            order(110,112,129)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238200"
     CDS             1..298
                     /gene="LOC117606552"
                     /coded_by="XM_076689848.1:264..1160"
                     /db_xref="GeneID:117606552"
ORIGIN      
        1 mglqyfiflf tffysglyta tgadwsysge hgpkhwpglc ttgkkqspin ivtedavktd
       61 lgelkfikys fafagdvtnn ghsvkiqirp apirleganl pnsyileemh fhwpaehtvd
      121 vnrdvlevhf vhysnkyhnt smaaqydkgi avvaslfkls iednmdimpv inltelvskd
      181 vgkstrikgt mvlpswflpk dhttyyhydg slttpgcqes vmwfilsekl siseaqlnvf
      241 knvgtengtl cfnyrptqpl egrtvyhhle gysaastlps nllltclsll lckllhsk
//
LOCUS       XP_076545964             295 aa            linear   INV 03-SEP-2025
DEFINITION  carbonic anhydrase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545964
VERSION     XP_076545964.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689849.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="carbonic anhydrase 1 isoform X2"
                     /calculated_mol_wt=32819
     Region          22..261
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; smart01057"
                     /db_xref="CDD:215000"
     Site            order(79,105,107,109,113,126,210)
                     /site_type="active"
                     /db_xref="CDD:238200"
     Site            order(107,109,126)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238200"
     CDS             1..295
                     /gene="LOC117606552"
                     /coded_by="XM_076689849.1:323..1210"
                     /db_xref="GeneID:117606552"
ORIGIN      
        1 mkslciivvt viglytatga dwsysgehgp khwpglcttg kkqspinivt edavktdlge
       61 lkfikysfaf agdvtnnghs vkiqirpapi rleganlpns yileemhfhw paehtvdvnr
      121 dvlevhfvhy snkyhntsma aqydkgiavv aslfklsied nmdimpvinl telvskdvgk
      181 strikgtmvl pswflpkdht tyyhydgslt tpgcqesvmw filseklsis eaqlnvfknv
      241 gtengtlcfn yrptqplegr tvyhhlegys aastlpsnll ltclslllck llhsk
//
LOCUS       XP_076545965             241 aa            linear   INV 03-SEP-2025
DEFINITION  carbonic anhydrase 6 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545965
VERSION     XP_076545965.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689850.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="carbonic anhydrase 6 isoform X3"
                     /calculated_mol_wt=27012
     Region          25..236
                     /region_name="Carb_anhydrase"
                     /note="Eukaryotic-type carbonic anhydrase; smart01057"
                     /db_xref="CDD:215000"
     Site            order(82,108,110,112,116,129,213)
                     /site_type="active"
                     /db_xref="CDD:238200"
     Site            order(110,112,129)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:238200"
     CDS             1..241
                     /gene="LOC117606552"
                     /coded_by="XM_076689850.1:267..992"
                     /db_xref="GeneID:117606552"
ORIGIN      
        1 mglqyfiflf tffysglyta tgadwsysge hgpkhwpglc ttgkkqspin ivtedavktd
       61 lgelkfikys fafagdvtnn ghsvkiqirp apirleganl pnsyileemh fhwpaehtvd
      121 vnrdvlevhf vhysnkyhnt smaaqydkgi avvaslfkls iednmdimpv inltelvskd
      181 vgkstrikgt mvlpswflpk dhttyyhydg slttpgcqes vmwfilsekl siseaqtnsa
      241 s
//
LOCUS       XP_034185027             269 aa            linear   INV 03-SEP-2025
DEFINITION  buttonless [Osmia lignaria lignaria].
ACCESSION   XP_034185027
VERSION     XP_034185027.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329136.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="buttonless"
                     /calculated_mol_wt=31118
     Region          198..254
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..269
                     /gene="btn"
                     /coded_by="XM_034329136.2:1932..2741"
                     /db_xref="GeneID:117606565"
ORIGIN      
        1 mswfedtess nqrssenilg tsptssilps hqnsyfagnw dsswnlkcpw nnfsnscnst
       61 plinplnepn ltpsctgnfq ygdsnrnyki yrleqcshqn gecniierst dkmdfdsseq
      121 slnficektq drqitttttt tvfraweips fqdaegkten eikccswqdp indsfekeac
      181 smerehetsk rlvgdkprke rtaftkqqvr hlecefahsn yltrlrryei avaldlterq
      241 vkvwfqnrrm kwkrtkaglg ngseklnvs
//
LOCUS       XP_034185028             269 aa            linear   INV 03-SEP-2025
DEFINITION  buttonless [Osmia lignaria lignaria].
ACCESSION   XP_034185028
VERSION     XP_034185028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="buttonless"
                     /calculated_mol_wt=31118
     Region          198..254
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..269
                     /gene="btn"
                     /coded_by="XM_034329137.2:57..866"
                     /db_xref="GeneID:117606565"
ORIGIN      
        1 mswfedtess nqrssenilg tsptssilps hqnsyfagnw dsswnlkcpw nnfsnscnst
       61 plinplnepn ltpsctgnfq ygdsnrnyki yrleqcshqn gecniierst dkmdfdsseq
      121 slnficektq drqitttttt tvfraweips fqdaegkten eikccswqdp indsfekeac
      181 smerehetsk rlvgdkprke rtaftkqqvr hlecefahsn yltrlrryei avaldlterq
      241 vkvwfqnrrm kwkrtkaglg ngseklnvs
//
LOCUS       XP_034185029             237 aa            linear   INV 03-SEP-2025
DEFINITION  MKI67 FHA domain-interacting nucleolar phosphoprotein-like [Osmia
            lignaria lignaria].
ACCESSION   XP_034185029
VERSION     XP_034185029.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="MKI67 FHA domain-interacting nucleolar
                     phosphoprotein-like"
                     /calculated_mol_wt=27531
     Region          85..158
                     /region_name="RRM_NIFK_like"
                     /note="RNA recognition motif in nucleolar protein
                     interacting with the FHA domain of pKI-67 (NIFK) and
                     similar proteins; cd12307"
                     /db_xref="CDD:409748"
     CDS             1..237
                     /gene="LOC117606567"
                     /coded_by="XM_034329138.2:75..788"
                     /db_xref="GeneID:117606567"
ORIGIN      
        1 mkikkrsnai rippikkaik virkssdgee estvskaiqn vkkvfekrna ekpkllssrk
       61 iikkrkvfvp vkekkvrerk strgivylgh iphgfyeeqm teyfkqfgqv trvrvtrsrn
      121 tgrsrgygyv efmhpdvaki aaetmnnylm cgrllkatyi ppekqhiryf agkpwteety
      181 pklrnrssvt eirnkdidht khssfvqstk dklsnlerkl kgkgfdlkft pvddtks
//
LOCUS       XP_034185016             212 aa            linear   INV 03-SEP-2025
DEFINITION  ER lumen protein-retaining receptor [Osmia lignaria lignaria].
ACCESSION   XP_034185016
VERSION     XP_034185016.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329125.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..212
                     /product="ER lumen protein-retaining receptor"
                     /calculated_mol_wt=24397
     Region          28..169
                     /region_name="ER_lumen_recept"
                     /note="ER lumen protein retaining receptor; pfam00810"
                     /db_xref="CDD:459945"
     CDS             1..212
                     /gene="KdelR"
                     /coded_by="XM_034329125.2:129..767"
                     /db_xref="GeneID:117606558"
ORIGIN      
        1 mnifrllgdl shllaiiill lkiwktrsca gisgksqilf aivyttryld littyisayn
       61 tfmkvifiat sfaivflmyv kfkatydhnh dtfrieflil ptlalallin heftvvevlw
      121 tfsiylesva ilpqlflvsk tgeaesitsh ylfalgsyrg lyllnwvyry yaehhydlia
      181 ivaglvqtvl ycdffylyit kvlkgkklql pa
//
LOCUS       XP_076545966            1099 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-mannosidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_076545966
VERSION     XP_076545966.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689851.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1099
                     /product="alpha-mannosidase 2"
                     /calculated_mol_wt=126236
     Region          82..1093
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     CDS             1..1099
                     /gene="LOC117608784"
                     /coded_by="XM_076689851.1:1180..4479"
                     /db_xref="GeneID:117608784"
ORIGIN      
        1 mykilrrgss rvlvvcavll slllclyyvs qaqgpstgps aailneelry drgltsitpd
       61 yiespdakvs ldtcpiivpr nvdidtqmef ekfdfqpswm rsreywddsf earyaelrkd
      121 ptrpplkvil vphshtdpgw lktfeqyfhs strnilnnmv sklqqwpnmt fiwsevsfls
      181 lwwesahptk kmvvkrlvkd grlemttggw vmtdeatshi yamldqlieg hqwlktnldv
      241 ipesgwsvdp fghggtipyf lkvsgasgtv iqrihyawkq wfakkqygdf vwmqpwdqtg
      301 eadmlthnqp fdiynikhsc gphphvclnf dfrkirgeyt eysvraveit pnnvkqmael
      361 lleqysrtgs lfthnvvlmp lgddfrydha iewdqqytny kilmdyinsr kdeynaeivf
      421 gtpkdyfrei rkrmndfptl kgdffvysdi fsegrpayws gyfttrpymk ildreleanl
      481 rsaeilytia lnvakqsgkd fmlyetyfek lvkarrnlgl fqhhdaitgt sksfvmkdya
      541 lklfesisdt tslqsfaiqs lagtttkpns vyvlaesdrd syeklpkkip igvngqdtkk
      601 ivlfnplaqp rqevislkvt sykikvldpq knpipyqiap vmnatsithd vyvllfmvel
      661 kplaiatyhi qqidkvpmea istvycsrcg kdsvfpmkpl qvgdvqlenq rmkllfdgqt
      721 gflkrvtkka tgkimqctiq faaypsaqfh sgaylfmpdp nlrdtdkdvl eaytphqkiy
      781 ivsgnlssrl tveygkllth hvaiyhrdgg lgeaiyvrni vdfetppknr etemfmrlqt
      841 dilngdppef ytdlnghqmi krtkierigi egnyfpittm ayiedtnhrl tllvnhcqga
      901 asyqpgwlev mldrrtlydd srgmgeglld nrrtvikhwl lleditgekd kyskpslfan
      961 hlsnalnypv nifvvdgtdg diamvpevrl lsqsfpcdvh llnlrtnhdq klphfpvnna
     1021 lmvlhrqgys csvgvditlk hcpltekipq gtsffklanl nitktsltgt ktmhrlrngl
     1081 qdvslqpmev etfnvnfvq
//
LOCUS       XP_076545967            1099 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-mannosidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_076545967
VERSION     XP_076545967.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689852.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1099
                     /product="alpha-mannosidase 2"
                     /calculated_mol_wt=126236
     Region          82..1093
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     CDS             1..1099
                     /gene="LOC117608784"
                     /coded_by="XM_076689852.1:970..4269"
                     /db_xref="GeneID:117608784"
ORIGIN      
        1 mykilrrgss rvlvvcavll slllclyyvs qaqgpstgps aailneelry drgltsitpd
       61 yiespdakvs ldtcpiivpr nvdidtqmef ekfdfqpswm rsreywddsf earyaelrkd
      121 ptrpplkvil vphshtdpgw lktfeqyfhs strnilnnmv sklqqwpnmt fiwsevsfls
      181 lwwesahptk kmvvkrlvkd grlemttggw vmtdeatshi yamldqlieg hqwlktnldv
      241 ipesgwsvdp fghggtipyf lkvsgasgtv iqrihyawkq wfakkqygdf vwmqpwdqtg
      301 eadmlthnqp fdiynikhsc gphphvclnf dfrkirgeyt eysvraveit pnnvkqmael
      361 lleqysrtgs lfthnvvlmp lgddfrydha iewdqqytny kilmdyinsr kdeynaeivf
      421 gtpkdyfrei rkrmndfptl kgdffvysdi fsegrpayws gyfttrpymk ildreleanl
      481 rsaeilytia lnvakqsgkd fmlyetyfek lvkarrnlgl fqhhdaitgt sksfvmkdya
      541 lklfesisdt tslqsfaiqs lagtttkpns vyvlaesdrd syeklpkkip igvngqdtkk
      601 ivlfnplaqp rqevislkvt sykikvldpq knpipyqiap vmnatsithd vyvllfmvel
      661 kplaiatyhi qqidkvpmea istvycsrcg kdsvfpmkpl qvgdvqlenq rmkllfdgqt
      721 gflkrvtkka tgkimqctiq faaypsaqfh sgaylfmpdp nlrdtdkdvl eaytphqkiy
      781 ivsgnlssrl tveygkllth hvaiyhrdgg lgeaiyvrni vdfetppknr etemfmrlqt
      841 dilngdppef ytdlnghqmi krtkierigi egnyfpittm ayiedtnhrl tllvnhcqga
      901 asyqpgwlev mldrrtlydd srgmgeglld nrrtvikhwl lleditgekd kyskpslfan
      961 hlsnalnypv nifvvdgtdg diamvpevrl lsqsfpcdvh llnlrtnhdq klphfpvnna
     1021 lmvlhrqgys csvgvditlk hcpltekipq gtsffklanl nitktsltgt ktmhrlrngl
     1081 qdvslqpmev etfnvnfvq
//
LOCUS       XP_076545968            1099 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-mannosidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_076545968
VERSION     XP_076545968.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689853.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1099
                     /product="alpha-mannosidase 2"
                     /calculated_mol_wt=126236
     Region          82..1093
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     CDS             1..1099
                     /gene="LOC117608784"
                     /coded_by="XM_076689853.1:876..4175"
                     /db_xref="GeneID:117608784"
ORIGIN      
        1 mykilrrgss rvlvvcavll slllclyyvs qaqgpstgps aailneelry drgltsitpd
       61 yiespdakvs ldtcpiivpr nvdidtqmef ekfdfqpswm rsreywddsf earyaelrkd
      121 ptrpplkvil vphshtdpgw lktfeqyfhs strnilnnmv sklqqwpnmt fiwsevsfls
      181 lwwesahptk kmvvkrlvkd grlemttggw vmtdeatshi yamldqlieg hqwlktnldv
      241 ipesgwsvdp fghggtipyf lkvsgasgtv iqrihyawkq wfakkqygdf vwmqpwdqtg
      301 eadmlthnqp fdiynikhsc gphphvclnf dfrkirgeyt eysvraveit pnnvkqmael
      361 lleqysrtgs lfthnvvlmp lgddfrydha iewdqqytny kilmdyinsr kdeynaeivf
      421 gtpkdyfrei rkrmndfptl kgdffvysdi fsegrpayws gyfttrpymk ildreleanl
      481 rsaeilytia lnvakqsgkd fmlyetyfek lvkarrnlgl fqhhdaitgt sksfvmkdya
      541 lklfesisdt tslqsfaiqs lagtttkpns vyvlaesdrd syeklpkkip igvngqdtkk
      601 ivlfnplaqp rqevislkvt sykikvldpq knpipyqiap vmnatsithd vyvllfmvel
      661 kplaiatyhi qqidkvpmea istvycsrcg kdsvfpmkpl qvgdvqlenq rmkllfdgqt
      721 gflkrvtkka tgkimqctiq faaypsaqfh sgaylfmpdp nlrdtdkdvl eaytphqkiy
      781 ivsgnlssrl tveygkllth hvaiyhrdgg lgeaiyvrni vdfetppknr etemfmrlqt
      841 dilngdppef ytdlnghqmi krtkierigi egnyfpittm ayiedtnhrl tllvnhcqga
      901 asyqpgwlev mldrrtlydd srgmgeglld nrrtvikhwl lleditgekd kyskpslfan
      961 hlsnalnypv nifvvdgtdg diamvpevrl lsqsfpcdvh llnlrtnhdq klphfpvnna
     1021 lmvlhrqgys csvgvditlk hcpltekipq gtsffklanl nitktsltgt ktmhrlrngl
     1081 qdvslqpmev etfnvnfvq
//
LOCUS       XP_076545969            1099 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-mannosidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_076545969
VERSION     XP_076545969.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689854.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1099
                     /product="alpha-mannosidase 2"
                     /calculated_mol_wt=126236
     Region          82..1093
                     /region_name="PLN02701"
                     /note="alpha-mannosidase"
                     /db_xref="CDD:178304"
     CDS             1..1099
                     /gene="LOC117608784"
                     /coded_by="XM_076689854.1:887..4186"
                     /db_xref="GeneID:117608784"
ORIGIN      
        1 mykilrrgss rvlvvcavll slllclyyvs qaqgpstgps aailneelry drgltsitpd
       61 yiespdakvs ldtcpiivpr nvdidtqmef ekfdfqpswm rsreywddsf earyaelrkd
      121 ptrpplkvil vphshtdpgw lktfeqyfhs strnilnnmv sklqqwpnmt fiwsevsfls
      181 lwwesahptk kmvvkrlvkd grlemttggw vmtdeatshi yamldqlieg hqwlktnldv
      241 ipesgwsvdp fghggtipyf lkvsgasgtv iqrihyawkq wfakkqygdf vwmqpwdqtg
      301 eadmlthnqp fdiynikhsc gphphvclnf dfrkirgeyt eysvraveit pnnvkqmael
      361 lleqysrtgs lfthnvvlmp lgddfrydha iewdqqytny kilmdyinsr kdeynaeivf
      421 gtpkdyfrei rkrmndfptl kgdffvysdi fsegrpayws gyfttrpymk ildreleanl
      481 rsaeilytia lnvakqsgkd fmlyetyfek lvkarrnlgl fqhhdaitgt sksfvmkdya
      541 lklfesisdt tslqsfaiqs lagtttkpns vyvlaesdrd syeklpkkip igvngqdtkk
      601 ivlfnplaqp rqevislkvt sykikvldpq knpipyqiap vmnatsithd vyvllfmvel
      661 kplaiatyhi qqidkvpmea istvycsrcg kdsvfpmkpl qvgdvqlenq rmkllfdgqt
      721 gflkrvtkka tgkimqctiq faaypsaqfh sgaylfmpdp nlrdtdkdvl eaytphqkiy
      781 ivsgnlssrl tveygkllth hvaiyhrdgg lgeaiyvrni vdfetppknr etemfmrlqt
      841 dilngdppef ytdlnghqmi krtkierigi egnyfpittm ayiedtnhrl tllvnhcqga
      901 asyqpgwlev mldrrtlydd srgmgeglld nrrtvikhwl lleditgekd kyskpslfan
      961 hlsnalnypv nifvvdgtdg diamvpevrl lsqsfpcdvh llnlrtnhdq klphfpvnna
     1021 lmvlhrqgys csvgvditlk hcpltekipq gtsffklanl nitktsltgt ktmhrlrngl
     1081 qdvslqpmev etfnvnfvq
//
LOCUS       XP_034190207            1606 aa            linear   INV 03-SEP-2025
DEFINITION  5'-3' exoribonuclease pacman [Osmia lignaria lignaria].
ACCESSION   XP_034190207
VERSION     XP_034190207.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334316.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190207.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1606
                     /product="5'-3' exoribonuclease pacman"
                     /calculated_mol_wt=185157
     Region          1..727
                     /region_name="XRN1"
                     /note="5'-3' exonuclease [Replication, recombination and
                     repair]; COG5049"
                     /db_xref="CDD:227382"
     Region          643..825
                     /region_name="XRN1_D1"
                     /note="Exoribonuclease Xrn1 D1 domain; pfam18332"
                     /db_xref="CDD:436418"
     Region          837..920
                     /region_name="XRN1_D2_D3"
                     /note="Exoribonuclease Xrn1 D2/D3 domain; pfam18334"
                     /db_xref="CDD:436419"
     Region          1087..1158
                     /region_name="SH3_12"
                     /note="Xrn1 SH3-like domain; pfam18129"
                     /db_xref="CDD:465661"
     CDS             1..1606
                     /gene="pcm"
                     /coded_by="XM_034334316.2:174..4994"
                     /db_xref="GeneID:117608750"
ORIGIN      
        1 mgipkfyryi serypclnet lkdyqipefd nlyldvngii hgcshpndsd vsfrisehvi
       61 fenifryiel lfrmiqprkl ffiaidgvap rakinqqrgr rfrsakdaei leakaraqgi
      121 eipkeerfds naitpgtefm dkvnkhlryl vtykistdkq wqkckvilsg sevpgegehk
      181 imdyirymka qpdydihtkh clygldadli mlglctheih ftllreeivy gkkqtklltp
      241 eetkfclfhl sllreyinhe fssmreklpf pydlekiidd wilmgflvgn dfipnlpnld
      301 imhgalsily qvymdvlptl egyineagtl kldrfekfme rlscfdilkf sehyadlkyl
      361 esktgrplte tdkpnhkkse gneqnlspkk mqdkefcall raavdmslgn edelgddesd
      421 setytmefiq hkrdyyinkl kyedinedfl rsqaegyvta lqwnlhyyyn gccswtwyyp
      481 hhyapyisdi kdfkdlkfef efgepflpfe qllavlppys kkllppafqs lltdeqspii
      541 dyypvefetd lngkrqewea vvlipfidst rlqdamapyy vkltpdeqar nkhgsmycyt
      601 hstqsmgrye avsehfhdfv stanvmaihr ediivprekl vkglcngvqv elhypgfptl
      661 qyiehtatle kakvkvfqrq skedsmilhi tppaeldltk iasellgktv fvnwphitea
      721 qvtavsncdt klslinpkmn yfsenvnree ikgplvsqwn lirkhinety mgrlgievgd
      781 inilvyvrsl igyryvirhg wlclekqwsd tqaahpyqtv vkdisvhsap aspykniesv
      841 fesgctffml gqpyygarge vldptvyrkt grvtvfvlik pepslekikq vqadlrtkym
      901 qgsiaakrlg isslllskit gtiyvkktpd efqqddtkyn iglnlkfnkk neeipgytrk
      961 engqwlyssk vidlirmymv kcpdlferla qninndffle eklfnkdtce mndimawlke
     1021 qlqgvenrac gvealdpsiv ktieetidpf lkaqknikhk pvvmnvkphl lfkpdlhlrk
     1081 ippdpkaetf lldriccike nftvplgykg tvigiqkmen fidtmydvvf dkifkdglsi
     1141 sgctegrgyr laptdfinis ygermkhska gtpevntesi dssnhapsqa aqscanasaf
     1201 asyekqtnls stspkipstm kptsefqalw nelhkkqpni tppqapmdpk vkqvnkgfpd
     1261 rtphdpsefl kamlkisdgn tqqstqqntq cripkpplkn taiqvenktl dtpplvqqlf
     1321 dharqterie dgkssiwycl qlveyyqfrr egmprytylt dkdtnfirah irlpdgrvfv
     1381 gdlcadhgea agnvarkvye elkvvnvlgk sprlpiphpf gpgrqniswl pnvlmppmvp
     1441 tpdystesrl ppyfrkstpk pqhnqvyqqa vpkqmnqynk hhqpqsklnh nevkteikdp
     1501 rsfvplqaqk ksrniankqt dnkdankcak dnpqsvmqqq kkelptkttk vevtttiips
     1561 ekkeqaqsnl hkvqntqnvi kpkksrvaak iftpskiqen hsndtt
//
LOCUS       XP_034190209             932 aa            linear   INV 03-SEP-2025
DEFINITION  importin-13-like protein cdm isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190209
VERSION     XP_034190209.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334318.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..932
                     /product="importin-13-like protein cdm isoform X1"
                     /calculated_mol_wt=104817
     Region          25..88
                     /region_name="IBN_N"
                     /note="Importin-beta N-terminal domain; smart00913"
                     /db_xref="CDD:197981"
     Region          <400..427
                     /region_name="Importin_rep"
                     /note="Importin 13 repeat; pfam18773"
                     /db_xref="CDD:436727"
     Region          798..869
                     /region_name="Importin_rep_3"
                     /note="Importin 13 repeat; pfam18806"
                     /db_xref="CDD:465873"
     CDS             1..932
                     /gene="cdm"
                     /coded_by="XM_034334318.2:451..3249"
                     /db_xref="GeneID:117608751"
ORIGIN      
        1 mdyatvidqa vkqfyaegnn evhswllrvq tspeawtfvw elldssksme aqfygattlh
       61 akiskqwnev pkneypalqe rllnfmkqpn tpkvvlsklc qalacfvant talenegkdk
      121 nivdelmgml sydsfpmlel llltlsvlpa eierryevrk pklyeflfng wcktawllqq
      181 vfsmcnpnsq dcdhelhlla lectlcwqkv gqlpleptgl iyphlliaaa yyapsrelcg
      241 eetvkgwevv qeclniivth yelkkrpqtl wewaqslvtm arqysgkyfc eiltaigeah
      301 sriflhalve genetqkwta ktlielllec seqegrypvd etrscipfgf wfalqddlst
      361 ldqpvenral qtlkpvyarl aqallrkstl psspneagda eerelfrryr qdvadtldyc
      421 yrvlgqdllv llgqrlsqtl dnsdkwtdve stlhafeala dsvgteeshy ipalmdlils
      481 hipydlypge vlacacstmg ayaewigehp dpwlervlri vtlgltrgsv tapfasmalk
      541 dlarecgqql tpfagtvlnt ieqtlpnvtp gcaeglrmmy aagkllntlp tvdeqlahld
      601 atlgmciiki rellkqpcfv argavinqlk mvtmffstle gsigkavldg llpifsqiva
      661 hpewgqdnft leemyicaqk sltsllhpgv darplltilr tsyktwphpt aldllrqlvl
      721 lfgrdpdnvi gpvfaelssi tlsgvracrs vrgnlsdwae lmeaylglla qickkntgml
      781 lkipdqipdm lqcgidcltl petgtvkaag nflthaimqs phlqtfiqpi gqqlvsvilq
      841 ciggevprnn lephaevlla lnktclewta qwlriafdkh salfsvsqpq keafvrnvlr
      901 erinkrmsdl lkdfslqnla sanklhittc kq
//
LOCUS       XP_034190210             806 aa            linear   INV 03-SEP-2025
DEFINITION  importin-13-like protein cdm isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190210
VERSION     XP_034190210.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334319.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..806
                     /product="importin-13-like protein cdm isoform X2"
                     /calculated_mol_wt=90437
     Region          <274..301
                     /region_name="Importin_rep"
                     /note="Importin 13 repeat; pfam18773"
                     /db_xref="CDD:436727"
     Region          672..743
                     /region_name="Importin_rep_3"
                     /note="Importin 13 repeat; pfam18806"
                     /db_xref="CDD:465873"
     CDS             1..806
                     /gene="cdm"
                     /coded_by="XM_034334319.2:194..2614"
                     /db_xref="GeneID:117608751"
ORIGIN      
        1 mgmlsydsfp mlelllltls vlpaeierry evrkpklyef lfngwcktaw llqqvfsmcn
       61 pnsqdcdhel hllalectlc wqkvgqlple ptgliyphll iaaayyapsr elcgeetvkg
      121 wevvqeclni ivthyelkkr pqtlwewaqs lvtmarqysg kyfceiltai geahsriflh
      181 alvegenetq kwtaktliel llecseqegr ypvdetrsci pfgfwfalqd dlstldqpve
      241 nralqtlkpv yarlaqallr kstlpsspne agdaeerelf rryrqdvadt ldycyrvlgq
      301 dllvllgqrl sqtldnsdkw tdvestlhaf ealadsvgte eshyipalmd lilshipydl
      361 ypgevlacac stmgayaewi gehpdpwler vlrivtlglt rgsvtapfas malkdlarec
      421 gqqltpfagt vlntieqtlp nvtpgcaegl rmmyaagkll ntlptvdeql ahldatlgmc
      481 iikirellkq pcfvargavi nqlkmvtmff stlegsigka vldgllpifs qivahpewgq
      541 dnftleemyi caqksltsll hpgvdarpll tilrtsyktw phptaldllr qlvllfgrdp
      601 dnvigpvfae lssitlsgvr acrsvrgnls dwaelmeayl gllaqickkn tgmllkipdq
      661 ipdmlqcgid cltlpetgtv kaagnfltha imqsphlqtf iqpigqqlvs vilqciggev
      721 prnnlephae vllalnktcl ewtaqwlria fdkhsalfsv sqpqkeafvr nvlrerinkr
      781 msdllkdfsl qnlasanklh ittckq
//
LOCUS       XP_034190211             189 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein C1orf50 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034190211
VERSION     XP_034190211.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334320.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="uncharacterized protein C1orf50 homolog"
                     /calculated_mol_wt=21212
     Region          17..171
                     /region_name="DUF2452"
                     /note="Protein of unknown function (DUF2452); pfam10504"
                     /db_xref="CDD:463120"
     CDS             1..189
                     /gene="LOC117608752"
                     /coded_by="XM_034334320.2:171..740"
                     /db_xref="GeneID:117608752"
ORIGIN      
        1 mciytimdns ddmcskvslv ernvepqglp lndpeavakt sqqdlvalal eiqkadsfik
       61 anacsklqvi adqikslkkq aenilletdw nmklhhvacn fvkhpghvyh lyqretgqly
      121 fsmlspeewg ssgpsqiykg ayrleqdhtw tplhltdkkn relamlaklf sthpttasvv
      181 ksidlnvdt
//
LOCUS       XP_076545971             189 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein C1orf50 homolog [Osmia lignaria lignaria].
ACCESSION   XP_076545971
VERSION     XP_076545971.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689856.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="uncharacterized protein C1orf50 homolog"
                     /calculated_mol_wt=21212
     Region          17..171
                     /region_name="DUF2452"
                     /note="Protein of unknown function (DUF2452); pfam10504"
                     /db_xref="CDD:463120"
     CDS             1..189
                     /gene="LOC117608752"
                     /coded_by="XM_076689856.1:171..740"
                     /db_xref="GeneID:117608752"
ORIGIN      
        1 mciytimdns ddmcskvslv ernvepqglp lndpeavakt sqqdlvalal eiqkadsfik
       61 anacsklqvi adqikslkkq aenilletdw nmklhhvacn fvkhpghvyh lyqretgqly
      121 fsmlspeewg ssgpsqiykg ayrleqdhtw tplhltdkkn relamlaklf sthpttasvv
      181 ksidlnvdt
//
LOCUS       XP_076545972             189 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein C1orf50 homolog [Osmia lignaria lignaria].
ACCESSION   XP_076545972
VERSION     XP_076545972.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689857.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="uncharacterized protein C1orf50 homolog"
                     /calculated_mol_wt=21212
     Region          17..171
                     /region_name="DUF2452"
                     /note="Protein of unknown function (DUF2452); pfam10504"
                     /db_xref="CDD:463120"
     CDS             1..189
                     /gene="LOC117608752"
                     /coded_by="XM_076689857.1:171..740"
                     /db_xref="GeneID:117608752"
ORIGIN      
        1 mciytimdns ddmcskvslv ernvepqglp lndpeavakt sqqdlvalal eiqkadsfik
       61 anacsklqvi adqikslkkq aenilletdw nmklhhvacn fvkhpghvyh lyqretgqly
      121 fsmlspeewg ssgpsqiykg ayrleqdhtw tplhltdkkn relamlaklf sthpttasvv
      181 ksidlnvdt
//
LOCUS       XP_034184645            2086 aa            linear   INV 03-SEP-2025
DEFINITION  AT hook containing transcription factor 1 homolog isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184645
VERSION     XP_034184645.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328754.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184645.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2086
                     /product="AT hook containing transcription factor 1
                     homolog isoform X1"
                     /calculated_mol_wt=237372
     Region          725..954
                     /region_name="ELYS"
                     /note="Nuclear pore complex assembly; pfam13934"
                     /db_xref="CDD:464050"
     Region          <1335..1963
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..2086
                     /gene="Elys"
                     /coded_by="XM_034328754.2:301..6561"
                     /db_xref="GeneID:117606395"
ORIGIN      
        1 mreleevnce irnvieiqqf mtsylsnieg dgtgslngsq icngvddvyg gflndaayaw
       61 lgygchliil ntksgencss wtfrgritcv cqfpahsgel plllvgldne atrtkdsmgl
      121 lcvfdcttsr vlrairmpag veqvcivsgg aeweefndkr pdniltqmdg iacvvlrnlh
      181 hlmidlqrss weipessvlm devspaeief ittkdsfhrn qtnknkhmac nllnrrieth
      241 igfnredfes nvildekltt svisstkigc lisgclgrvi iwqndgsvgw isspvdetmv
      301 vtqlallept ddprpfyylw vvfqddtfkv ppllrmyall ferkysdkrt nfyfnleaep
      361 clkfefkldt adrvvslsti eretnldqte sefrrseesl llisttnrtl lfdlnqwyke
      421 qmpqtineck npnsilacyd tkhrpsssnr dkiisstyvl rslqefpnnn lslseeifyp
      481 nslslewiel sltkltfwlt rgvqnellre ialtgpiilt qpsemfhkcl svglipfnte
      541 vsfssdqnvq rdmilslcle qrwttflikc akewsdgsaa ymypnflkwg iqrassikli
      601 adrlcvplfd qsgnsigesd vktlrfcyqq leclsnvvak lpfetsslmk qrkalkrvsm
      661 yfqvllwfyd vgllpetqsl eegslpisls lkipypfekl ynlykekres irddnetden
      721 vrvffideli arecpalnlq wekeagdtnt ngnypppslq sllrsyltdc dqaesdeiec
      781 khqiiiyllm dlamllqgsy pgvdqlikyp ssfklspsli kltqafwlld hedyhgfldm
      841 mtgqlvrdsd vkpwhhklvl rtlirnnqhk lalmylrikk pplssvqeqs tliglsvehg
      901 liqsafhhrp qshyaqlltc ffqacktynk lsdilhlald seeeemfvkf ledtksedtr
      961 llyylqrcry meanngrsfd trnlhtdmln aynatlpdvv krfsmnmgkt sfdtglepry
     1021 pkpmshtkns qqitniyetm irkaketcpr gersfipfvt apctslrlnd drinincvvs
     1081 pkivqksrkr sfdrtmedee dsgmrtpdrt krrklldege aalnaafstp lvkrkvsssr
     1141 dtpsetphsi lkirqlirns tsptigslqg dtvdgteser krkinrqirf sisqskknna
     1201 hdetnaeedg skvnasegsd evslsptine ksltestvls dssytcknvy tprprpslrr
     1261 tylqtptesi nesrkrisan snlsnssnfv encsklasea tprlsrtcte lfsssihssa
     1321 vlspnssfev eislkrslen nsqllkfskq qnnynsinst vlrnssrsee qykveednev
     1381 qctenrsest eklnfqfged nrsredvlsk crngdlskar tleadneend heeefehfsn
     1441 atenksalsg skerwsvgrk lqnqsrqene nlnngehfgd itedessksg dedkdkekdg
     1501 sqkiqmkgdn mydasnitdd esdssteiie skrnsiaisn kqenvdklsd kkkytrddet
     1561 ieksicldnn nssyretvek avhdsqksvn lkddvntsnl dnesldtsqn itprvtrsrr
     1621 assiakeini splnsptkvi gktprsrras slmkevlias iapadeevrq tassgseess
     1681 pkrgrrrats vtkdvsekve deevkapvvr rstrraasvq kelsesnkpl aknskvslss
     1741 epimedekek lqteikktka rrgsvserkp gtpsksmkes klneekgeem nlkatrkhgs
     1801 svpkeamsai rtrrsssvvk edipedvell ekimkerhvv daavnspaan trsrrlsiqs
     1861 vpeeleevls vpskeratks katkqsaqts rqrratsvep yqaeskritr strgkdilke
     1921 viveeevpqm dspgkaeasg vkkvptkrkr tssttvttqe dkettprsrr grktknqgin
     1981 dqflfsqpdk pgdspldeka igevpkyvfs pphtrskaia pdyrsrmknf ipilnedeie
     2041 eeekeeerqs lqksskykkt gqytrvtthr yrtkfvlpkr skstsd
//
LOCUS       XP_034184646            2086 aa            linear   INV 03-SEP-2025
DEFINITION  AT hook containing transcription factor 1 homolog isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184646
VERSION     XP_034184646.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328755.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184646.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2086
                     /product="AT hook containing transcription factor 1
                     homolog isoform X1"
                     /calculated_mol_wt=237372
     Region          725..954
                     /region_name="ELYS"
                     /note="Nuclear pore complex assembly; pfam13934"
                     /db_xref="CDD:464050"
     Region          <1335..1963
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..2086
                     /gene="Elys"
                     /coded_by="XM_034328755.2:291..6551"
                     /db_xref="GeneID:117606395"
ORIGIN      
        1 mreleevnce irnvieiqqf mtsylsnieg dgtgslngsq icngvddvyg gflndaayaw
       61 lgygchliil ntksgencss wtfrgritcv cqfpahsgel plllvgldne atrtkdsmgl
      121 lcvfdcttsr vlrairmpag veqvcivsgg aeweefndkr pdniltqmdg iacvvlrnlh
      181 hlmidlqrss weipessvlm devspaeief ittkdsfhrn qtnknkhmac nllnrrieth
      241 igfnredfes nvildekltt svisstkigc lisgclgrvi iwqndgsvgw isspvdetmv
      301 vtqlallept ddprpfyylw vvfqddtfkv ppllrmyall ferkysdkrt nfyfnleaep
      361 clkfefkldt adrvvslsti eretnldqte sefrrseesl llisttnrtl lfdlnqwyke
      421 qmpqtineck npnsilacyd tkhrpsssnr dkiisstyvl rslqefpnnn lslseeifyp
      481 nslslewiel sltkltfwlt rgvqnellre ialtgpiilt qpsemfhkcl svglipfnte
      541 vsfssdqnvq rdmilslcle qrwttflikc akewsdgsaa ymypnflkwg iqrassikli
      601 adrlcvplfd qsgnsigesd vktlrfcyqq leclsnvvak lpfetsslmk qrkalkrvsm
      661 yfqvllwfyd vgllpetqsl eegslpisls lkipypfekl ynlykekres irddnetden
      721 vrvffideli arecpalnlq wekeagdtnt ngnypppslq sllrsyltdc dqaesdeiec
      781 khqiiiyllm dlamllqgsy pgvdqlikyp ssfklspsli kltqafwlld hedyhgfldm
      841 mtgqlvrdsd vkpwhhklvl rtlirnnqhk lalmylrikk pplssvqeqs tliglsvehg
      901 liqsafhhrp qshyaqlltc ffqacktynk lsdilhlald seeeemfvkf ledtksedtr
      961 llyylqrcry meanngrsfd trnlhtdmln aynatlpdvv krfsmnmgkt sfdtglepry
     1021 pkpmshtkns qqitniyetm irkaketcpr gersfipfvt apctslrlnd drinincvvs
     1081 pkivqksrkr sfdrtmedee dsgmrtpdrt krrklldege aalnaafstp lvkrkvsssr
     1141 dtpsetphsi lkirqlirns tsptigslqg dtvdgteser krkinrqirf sisqskknna
     1201 hdetnaeedg skvnasegsd evslsptine ksltestvls dssytcknvy tprprpslrr
     1261 tylqtptesi nesrkrisan snlsnssnfv encsklasea tprlsrtcte lfsssihssa
     1321 vlspnssfev eislkrslen nsqllkfskq qnnynsinst vlrnssrsee qykveednev
     1381 qctenrsest eklnfqfged nrsredvlsk crngdlskar tleadneend heeefehfsn
     1441 atenksalsg skerwsvgrk lqnqsrqene nlnngehfgd itedessksg dedkdkekdg
     1501 sqkiqmkgdn mydasnitdd esdssteiie skrnsiaisn kqenvdklsd kkkytrddet
     1561 ieksicldnn nssyretvek avhdsqksvn lkddvntsnl dnesldtsqn itprvtrsrr
     1621 assiakeini splnsptkvi gktprsrras slmkevlias iapadeevrq tassgseess
     1681 pkrgrrrats vtkdvsekve deevkapvvr rstrraasvq kelsesnkpl aknskvslss
     1741 epimedekek lqteikktka rrgsvserkp gtpsksmkes klneekgeem nlkatrkhgs
     1801 svpkeamsai rtrrsssvvk edipedvell ekimkerhvv daavnspaan trsrrlsiqs
     1861 vpeeleevls vpskeratks katkqsaqts rqrratsvep yqaeskritr strgkdilke
     1921 viveeevpqm dspgkaeasg vkkvptkrkr tssttvttqe dkettprsrr grktknqgin
     1981 dqflfsqpdk pgdspldeka igevpkyvfs pphtrskaia pdyrsrmknf ipilnedeie
     2041 eeekeeerqs lqksskykkt gqytrvtthr yrtkfvlpkr skstsd
//
LOCUS       XP_034184647            2085 aa            linear   INV 03-SEP-2025
DEFINITION  AT hook containing transcription factor 1 homolog isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184647
VERSION     XP_034184647.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328756.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184647.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2085
                     /product="AT hook containing transcription factor 1
                     homolog isoform X2"
                     /calculated_mol_wt=237285
     Region          725..954
                     /region_name="ELYS"
                     /note="Nuclear pore complex assembly; pfam13934"
                     /db_xref="CDD:464050"
     Region          <1335..1963
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..2085
                     /gene="Elys"
                     /coded_by="XM_034328756.2:301..6558"
                     /db_xref="GeneID:117606395"
ORIGIN      
        1 mreleevnce irnvieiqqf mtsylsnieg dgtgslngsq icngvddvyg gflndaayaw
       61 lgygchliil ntksgencss wtfrgritcv cqfpahsgel plllvgldne atrtkdsmgl
      121 lcvfdcttsr vlrairmpag veqvcivsgg aeweefndkr pdniltqmdg iacvvlrnlh
      181 hlmidlqrss weipessvlm devspaeief ittkdsfhrn qtnknkhmac nllnrrieth
      241 igfnredfes nvildekltt svisstkigc lisgclgrvi iwqndgsvgw isspvdetmv
      301 vtqlallept ddprpfyylw vvfqddtfkv ppllrmyall ferkysdkrt nfyfnleaep
      361 clkfefkldt adrvvslsti eretnldqte sefrrseesl llisttnrtl lfdlnqwyke
      421 qmpqtineck npnsilacyd tkhrpsssnr dkiisstyvl rslqefpnnn lslseeifyp
      481 nslslewiel sltkltfwlt rgvqnellre ialtgpiilt qpsemfhkcl svglipfnte
      541 vsfssdqnvq rdmilslcle qrwttflikc akewsdgsaa ymypnflkwg iqrassikli
      601 adrlcvplfd qsgnsigesd vktlrfcyqq leclsnvvak lpfetsslmk qrkalkrvsm
      661 yfqvllwfyd vgllpetqsl eegslpisls lkipypfekl ynlykekres irddnetden
      721 vrvffideli arecpalnlq wekeagdtnt ngnypppslq sllrsyltdc dqaesdeiec
      781 khqiiiyllm dlamllqgsy pgvdqlikyp ssfklspsli kltqafwlld hedyhgfldm
      841 mtgqlvrdsd vkpwhhklvl rtlirnnqhk lalmylrikk pplssvqeqs tliglsvehg
      901 liqsafhhrp qshyaqlltc ffqacktynk lsdilhlald seeeemfvkf ledtksedtr
      961 llyylqrcry meanngrsfd trnlhtdmln aynatlpdvv krfsmnmgkt sfdtglepry
     1021 pkpmshtkns qqitniyetm irkaketcpr gersfipfvt apctslrlnd drinincvvs
     1081 pkivqksrkr sfdrtmedee dsgmrtpdrt krrklldege aalnaafstp lvkrkvsssr
     1141 dtpsetphsi lkirqlirns tsptigslqg dtvdgteser krkinrqirf sisqskknna
     1201 hdetnaeedg skvnasegsd evslsptine ksltestvls dssytcknvy tprprpslrr
     1261 tylqtptesi nesrkrisan snlsnssnfv encsklasea tprlsrtcte lfsssihssa
     1321 vlspnssfev eislkrslen nsqllkfskq qnnynsinst vlrnssrsee qykveednev
     1381 qctenrsest eklnfqfged nrsredvlsk crngdlskar tleadneend heeefehfsn
     1441 atenksalsg skerwsvgrk lqnqsrqene nlnngehfgd itedessksg dedkdkekdg
     1501 sqkiqmkgdn mydasnitdd esdssteiie skrnsiaisn kqenvdklsd kkkytrddet
     1561 ieksicldnn nssyretvek avhdsqksvn lkddvntsnl dnesldtsqn itprvtrsrr
     1621 assiakeini splnsptkvi gktprsrras slmkevlias iapadeevrq tassgseess
     1681 pkrgrrrats vtkdvsekve deevkapvvr rstrraasvq kelsesnkpl aknskvslss
     1741 epimedekek lqteikktka rrgsvserkp gtpsksmkes klneekgeem nlkatrkhgs
     1801 svpkeamsai rtrrsssvvk edipedvell ekimkerhvv daavnspaan trsrrlsiqs
     1861 vpeeleevls vpskeratks katkqsaqts rqrratsvep yqaeskritr strgkdilke
     1921 viveeevpqm dspgkaeasg vkkvptkrkr tssttvttqe dkettprsrr grktknqgin
     1981 dqflfsqpdk pgdspldeka igevpkyvfs pphtrskaia pdyrrmknfi pilnedeiee
     2041 eekeeerqsl qksskykktg qytrvtthry rtkfvlpkrs kstsd
//
LOCUS       XP_034184649            2025 aa            linear   INV 03-SEP-2025
DEFINITION  AT hook containing transcription factor 1 homolog isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184649
VERSION     XP_034184649.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328758.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184649.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2025
                     /product="AT hook containing transcription factor 1
                     homolog isoform X3"
                     /calculated_mol_wt=229950
     Region          725..954
                     /region_name="ELYS"
                     /note="Nuclear pore complex assembly; pfam13934"
                     /db_xref="CDD:464050"
     Region          <1335..1963
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..2025
                     /gene="Elys"
                     /coded_by="XM_034328758.2:301..6378"
                     /db_xref="GeneID:117606395"
ORIGIN      
        1 mreleevnce irnvieiqqf mtsylsnieg dgtgslngsq icngvddvyg gflndaayaw
       61 lgygchliil ntksgencss wtfrgritcv cqfpahsgel plllvgldne atrtkdsmgl
      121 lcvfdcttsr vlrairmpag veqvcivsgg aeweefndkr pdniltqmdg iacvvlrnlh
      181 hlmidlqrss weipessvlm devspaeief ittkdsfhrn qtnknkhmac nllnrrieth
      241 igfnredfes nvildekltt svisstkigc lisgclgrvi iwqndgsvgw isspvdetmv
      301 vtqlallept ddprpfyylw vvfqddtfkv ppllrmyall ferkysdkrt nfyfnleaep
      361 clkfefkldt adrvvslsti eretnldqte sefrrseesl llisttnrtl lfdlnqwyke
      421 qmpqtineck npnsilacyd tkhrpsssnr dkiisstyvl rslqefpnnn lslseeifyp
      481 nslslewiel sltkltfwlt rgvqnellre ialtgpiilt qpsemfhkcl svglipfnte
      541 vsfssdqnvq rdmilslcle qrwttflikc akewsdgsaa ymypnflkwg iqrassikli
      601 adrlcvplfd qsgnsigesd vktlrfcyqq leclsnvvak lpfetsslmk qrkalkrvsm
      661 yfqvllwfyd vgllpetqsl eegslpisls lkipypfekl ynlykekres irddnetden
      721 vrvffideli arecpalnlq wekeagdtnt ngnypppslq sllrsyltdc dqaesdeiec
      781 khqiiiyllm dlamllqgsy pgvdqlikyp ssfklspsli kltqafwlld hedyhgfldm
      841 mtgqlvrdsd vkpwhhklvl rtlirnnqhk lalmylrikk pplssvqeqs tliglsvehg
      901 liqsafhhrp qshyaqlltc ffqacktynk lsdilhlald seeeemfvkf ledtksedtr
      961 llyylqrcry meanngrsfd trnlhtdmln aynatlpdvv krfsmnmgkt sfdtglepry
     1021 pkpmshtkns qqitniyetm irkaketcpr gersfipfvt apctslrlnd drinincvvs
     1081 pkivqksrkr sfdrtmedee dsgmrtpdrt krrklldege aalnaafstp lvkrkvsssr
     1141 dtpsetphsi lkirqlirns tsptigslqg dtvdgteser krkinrqirf sisqskknna
     1201 hdetnaeedg skvnasegsd evslsptine ksltestvls dssytcknvy tprprpslrr
     1261 tylqtptesi nesrkrisan snlsnssnfv encsklasea tprlsrtcte lfsssihssa
     1321 vlspnssfev eislkrslen nsqllkfskq qnnynsinst vlrnssrsee qykveednev
     1381 qctenrsest eklnfqfged nrsredvlsk crngdlskar tleadneend heeefehfsn
     1441 atenksalsg skerwsvgrk lqnqsrqene nlnngehfgd itedessksg dedkdkekdg
     1501 sqkiqmkgdn mydasnitdd esdssteiie skrnsiaisn kqenvdklsd kkkytrddet
     1561 ieksicldnn nssyretvek avhdsqksvn lkddvntsnl dnesldtsqn itprvtrsrr
     1621 assiakeini splnsptkvi gktprsrras slmkevlias iapadeevrq tassgseess
     1681 pkrgrrrats vtkdvsekve deevkapvvr rstrraasvq kelsesnkpl aknskvslss
     1741 epimedekek lqteikktka rrgsvserkp gtpsksmkes klneekgeem nlkatrkhgs
     1801 svpkeamsai rtrrsssvvk edipedvell ekimkerhvv daavnspaan trsrrlsiqs
     1861 vpeeleevls vpskeratks katkqsaqts rqrratsvep yqaeskritr strgkdilke
     1921 viveeevpqm dspgkaeasg vkkvptkrkr tssttvttqe dkettprsrr grktknqgin
     1981 dqflfsqpdk pgdspldeka igevpkyvfs pphtrskaia pdype
//
LOCUS       XP_034184650            2024 aa            linear   INV 03-SEP-2025
DEFINITION  AT hook containing transcription factor 1 homolog isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184650
VERSION     XP_034184650.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328759.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184650.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2024
                     /product="AT hook containing transcription factor 1
                     homolog isoform X4"
                     /calculated_mol_wt=229851
     Region          725..954
                     /region_name="ELYS"
                     /note="Nuclear pore complex assembly; pfam13934"
                     /db_xref="CDD:464050"
     Region          <1335..1963
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..2024
                     /gene="Elys"
                     /coded_by="XM_034328759.2:301..6375"
                     /db_xref="GeneID:117606395"
ORIGIN      
        1 mreleevnce irnvieiqqf mtsylsnieg dgtgslngsq icngvddvyg gflndaayaw
       61 lgygchliil ntksgencss wtfrgritcv cqfpahsgel plllvgldne atrtkdsmgl
      121 lcvfdcttsr vlrairmpag veqvcivsgg aeweefndkr pdniltqmdg iacvvlrnlh
      181 hlmidlqrss weipessvlm devspaeief ittkdsfhrn qtnknkhmac nllnrrieth
      241 igfnredfes nvildekltt svisstkigc lisgclgrvi iwqndgsvgw isspvdetmv
      301 vtqlallept ddprpfyylw vvfqddtfkv ppllrmyall ferkysdkrt nfyfnleaep
      361 clkfefkldt adrvvslsti eretnldqte sefrrseesl llisttnrtl lfdlnqwyke
      421 qmpqtineck npnsilacyd tkhrpsssnr dkiisstyvl rslqefpnnn lslseeifyp
      481 nslslewiel sltkltfwlt rgvqnellre ialtgpiilt qpsemfhkcl svglipfnte
      541 vsfssdqnvq rdmilslcle qrwttflikc akewsdgsaa ymypnflkwg iqrassikli
      601 adrlcvplfd qsgnsigesd vktlrfcyqq leclsnvvak lpfetsslmk qrkalkrvsm
      661 yfqvllwfyd vgllpetqsl eegslpisls lkipypfekl ynlykekres irddnetden
      721 vrvffideli arecpalnlq wekeagdtnt ngnypppslq sllrsyltdc dqaesdeiec
      781 khqiiiyllm dlamllqgsy pgvdqlikyp ssfklspsli kltqafwlld hedyhgfldm
      841 mtgqlvrdsd vkpwhhklvl rtlirnnqhk lalmylrikk pplssvqeqs tliglsvehg
      901 liqsafhhrp qshyaqlltc ffqacktynk lsdilhlald seeeemfvkf ledtksedtr
      961 llyylqrcry meanngrsfd trnlhtdmln aynatlpdvv krfsmnmgkt sfdtglepry
     1021 pkpmshtkns qqitniyetm irkaketcpr gersfipfvt apctslrlnd drinincvvs
     1081 pkivqksrkr sfdrtmedee dsgmrtpdrt krrklldege aalnaafstp lvkrkvsssr
     1141 dtpsetphsi lkirqlirns tsptigslqg dtvdgteser krkinrqirf sisqskknna
     1201 hdetnaeedg skvnasegsd evslsptine ksltestvls dssytcknvy tprprpslrr
     1261 tylqtptesi nesrkrisan snlsnssnfv encsklasea tprlsrtcte lfsssihssa
     1321 vlspnssfev eislkrslen nsqllkfskq qnnynsinst vlrnssrsee qykveednev
     1381 qctenrsest eklnfqfged nrsredvlsk crngdlskar tleadneend heeefehfsn
     1441 atenksalsg skerwsvgrk lqnqsrqene nlnngehfgd itedessksg dedkdkekdg
     1501 sqkiqmkgdn mydasnitdd esdssteiie skrnsiaisn kqenvdklsd kkkytrddet
     1561 ieksicldnn nssyretvek avhdsqksvn lkddvntsnl dnesldtsqn itprvtrsrr
     1621 assiakeini splnsptkvi gktprsrras slmkevlias iapadeevrq tassgseess
     1681 pkrgrrrats vtkdvsekve deevkapvvr rstrraasvq kelsesnkpl aknskvslss
     1741 epimedekek lqteikktka rrgsvserkp gtpsksmkes klneekgeem nlkatrkhgs
     1801 svpkeamsai rtrrsssvvk edipedvell ekimkerhvv daavnspaan trsrrlsiqs
     1861 vpeeleevls vpskeratks katkqsaqts rqrratsvep yqaeskritr strgkdilke
     1921 viveeevpqm dspgkaeasg vkkvptkrkr tssttvttqe dkettprsrr grktknqgin
     1981 dqflfsqpdk pgdspldeka igevpkyvfs pphtrskaia pdyq
//
LOCUS       XP_034184652             709 aa            linear   INV 03-SEP-2025
DEFINITION  exocyst complex component Exo84 [Osmia lignaria lignaria].
ACCESSION   XP_034184652
VERSION     XP_034184652.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328761.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..709
                     /product="exocyst complex component Exo84"
                     /calculated_mol_wt=79501
     Region          9..95
                     /region_name="Vps51"
                     /note="Vps51/Vps67; pfam08700"
                     /db_xref="CDD:462568"
     Region          143..258
                     /region_name="PH_RalBD_exo84"
                     /note="Exocyst complex 84-kDa subunit Ral-binding
                     domain/Pleckstrin Homology (PH) domain; cd01226"
                     /db_xref="CDD:269933"
     Site            order(143..184,187..190,195..258)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:269933"
     Site            order(160..161,163,203..212,214,222,224..226,248,251,255,
                     258)
                     /site_type="other"
                     /note="RalA binding site [polypeptide binding]"
                     /db_xref="CDD:269933"
     Region          303..510
                     /region_name="Exo84_C"
                     /note="Exocyst component 84 C-terminal; pfam16528"
                     /db_xref="CDD:465161"
     CDS             1..709
                     /gene="Exo84"
                     /coded_by="XM_034328761.2:312..2441"
                     /db_xref="GeneID:117606396"
ORIGIN      
        1 madslakvft gedfnpekfv kelsaqcvga delrqqraki qelanntsaq lkrnvyqnym
       61 qfietakeis hlesemyqls qllseqrsll ttlgsarttg vifeessesq lenlndstsk
      121 eeeqrqkltq llenvegams laetpgrvcl hegslleldp fegtplkrvh aylfndilmi
      181 aswlangnrr gppkykmqav ynleslaivn irdlgtvkla fkllafsdtr vfqcatatsk
      241 kdwldkceqa kkmklaedns sgaadydkrs keektvpsrs msldsntlgi deedaeyhep
      301 ppewmlevae dldsciaqrh feeaysller akaylkdsqv tpllleiqsk vndrgkslvd
      361 vltkelessv eakslqgggl rsarrvvkll iqlnrsaqac hlylrlcsav lkarlkrvkk
      421 egtispyvkq lsaiafsniv eiakeflkif pqstnctsgl vvwcsqevkh ltthltkqif
      481 tpqvslstlv eciaavrshc dqltqlgmdf ryqldgqlrs plakaiqdag ekyvdivkvh
      541 vaedtwrptn letnknlqkl lselddlgvt vpssyltnnc wiqlsnntlt fsklyvslle
      601 dclsvatpel matidgvlvs vmraqvqhvc gsltnpklkq erqlvhdnas yirdvvimrg
      661 lelyksatnq tfkkllalke qivfdssspt kpksaprtsi pkystteyi
//
LOCUS       XP_034184653             348 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606397 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184653
VERSION     XP_034184653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328762.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..348
                     /product="uncharacterized protein LOC117606397 isoform X1"
                     /calculated_mol_wt=40525
     Region          16..153
                     /region_name="CCDC50_N"
                     /note="Coiled-coil domain-containing protein 50
                     N-terminus; pfam15295"
                     /db_xref="CDD:464621"
     Region          <85..>224
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..348
                     /gene="LOC117606397"
                     /coded_by="XM_034328762.2:143..1189"
                     /db_xref="GeneID:117606397"
ORIGIN      
        1 maksvlssdt lpkagrvnev crewlvhedg alayrlqdee ikehytgnkv rnaqvredlp
       61 rarveqelet lryqsyvqqh vscfreerda lvakqialsl ereerkkere lqeqmrlqlr
      121 lddeaaqlei nrhiqeekde elarklqlee eekavdthdg pvneqllldq kiameaqdae
      181 larmlqeker mkarkarqra kqrklerqql qelddqnpse kpqrpdkldl kngqsksrvq
      241 htqnyphysd peeiqtldes venmrevpnv aaiidptyng nlhrntssss sstvspthal
      301 ptpppelmnd dapcympiqg qrrnqiqsps qtheekhkrr vkdsckqq
//
LOCUS       XP_034184654             343 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606397 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184654
VERSION     XP_034184654.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328763.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..343
                     /product="uncharacterized protein LOC117606397 isoform X2"
                     /calculated_mol_wt=39924
     Region          16..148
                     /region_name="CCDC50_N"
                     /note="Coiled-coil domain-containing protein 50
                     N-terminus; pfam15295"
                     /db_xref="CDD:464621"
     CDS             1..343
                     /gene="LOC117606397"
                     /coded_by="XM_034328763.2:141..1172"
                     /db_xref="GeneID:117606397"
ORIGIN      
        1 maksvlssdt lpkagrvnev crewlvhedg alayrlqdee ikehytgnkv rnaqvredlp
       61 rarveqelet lryqsyvqqq eerdalvakq ialslereer kkerelqeqm rlqlrlddea
      121 aqleinrhiq eekdeelark lqleeeekav dthdgpvneq llldqkiame aqdaelarml
      181 qekermkark arqrakqrkl erqqlqeldd qnpsekpqrp dkldlkngqs ksrvqhtqny
      241 physdpeeiq tldesvenmr evpnvaaiid ptyngnlhrn tsssssstvs pthalptppp
      301 elmnddapcy mpiqgqrrnq iqspsqthee khkrrvkdsc kqq
//
LOCUS       XP_034184655             232 aa            linear   INV 03-SEP-2025
DEFINITION  sperm-associated antigen 7 [Osmia lignaria lignaria].
ACCESSION   XP_034184655
VERSION     XP_034184655.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328764.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..232
                     /product="sperm-associated antigen 7"
                     /calculated_mol_wt=27117
     Region          47..107
                     /region_name="R3H"
                     /note="R3H domain. The name of the R3H domain comes from
                     the characteristic spacing of the most conserved arginine
                     and histidine residues. R3H domains are found in proteins
                     together with ATPase domains, SF1 helicase domains, SF2
                     DEAH helicase domains, Cys-rich...; cl00297"
                     /db_xref="CDD:469711"
     Site            order(76,80)
                     /site_type="other"
                     /note="RxxxH motif"
                     /db_xref="CDD:100064"
     CDS             1..232
                     /gene="LOC117606398"
                     /coded_by="XM_034328764.2:124..822"
                     /db_xref="GeneID:117606398"
ORIGIN      
        1 mdllgsilns mdkpptvsdk qktlmkkqke eyqkyqraea erlksfrgkv edkinkflqd
       61 dnakeykfpp mdqihrsiih dvaevanvwa ysfgeegidr hivifkreha psedqlnvlr
      121 rgeewneeva nklveererr akeeieiaks kkrkdnftpn syykdkyqhl igkeaaleaa
      181 rkteanssyg cvpsenkkdq rsieqtladi rakkrkleet kdseckdkii kq
//
LOCUS       XP_034184640             786 aa            linear   INV 03-SEP-2025
DEFINITION  phospholipase A2 activator protein [Osmia lignaria lignaria].
ACCESSION   XP_034184640
VERSION     XP_034184640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328749.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..786
                     /product="phospholipase A2 activator protein"
                     /calculated_mol_wt=86610
     Region          21..311
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(28,45,49,55..56,72..73,96,100,106..107,119..120,137,
                     142,148..149,163,179,184,190,202..203,220,224,230..231,
                     243..244,261,266,272..273,283..284,301,305,311)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          34..72
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          78..119
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          124..161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          168..201
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          207..242
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          248..282
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          288..311
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          359..471
                     /region_name="PFU"
                     /note="PFU (PLAA family ubiquitin binding); pfam09070"
                     /db_xref="CDD:462670"
     Region          522..766
                     /region_name="PUL"
                     /note="PUL domain; pfam08324"
                     /db_xref="CDD:462428"
     CDS             1..786
                     /gene="Plap"
                     /coded_by="XM_034328749.2:218..2578"
                     /db_xref="GeneID:117606392"
ORIGIN      
        1 msllrashdy qntmakpcyk lrtslfghts dvravatfad gtivstsrde tarvwkpsgs
       61 gkdyehtttl kghsnfvtsv cvinpsernp tgfvitgshd ktihiyvpdq tepinviksh
      121 qdtvcklstg kkegiflsss wdmsakvwnl sdlskpqlnl lghtaavwcv idlssglyvt
      181 gsadklviiw tsdgsvqhkl tghtdcvrdi sainnneflt candatvrhw naslgtclgt
      241 ycghenyiys ivalengtni ftcgedrtlr iwnntelnqt itlptqsvwc iallpdgdvi
      301 agssdcvvri ftcnpehyad aetlkefeqe vasnklnaql elggikvkdl pdakallqpg
      361 qrdgqtkivn dgdairaysw sqneqrwiki gdvmgasggs aatsgkqlyn gkeydyvfsv
      421 diqdgvpplk lpynndqdpw hvaqkflldn glsqllldqv anfiiknsrp apvvkadaqy
      481 adpftggsry ipqattdtvp qecakpatin ssdtsltpsy iphikylkle qanlpailek
      541 lrelnskqsd plkvsneqle clvklagnka seqlrgdaln tlmvllnwsd dvlfpvldit
      601 rlvvlckdin dvlctdellq ivknhirnda lpsnqmlvfr llanmfayek geklcinykd
      661 eilrsildlq slgsknnqva istfilnltv alnkysdtvg ktqclnvifs vlprlnesea
      721 vfrtlvalgt llctttdqkd rdhlikavrq seaalnilkt msetkistnt snklancskq
      781 iislii
//
LOCUS       XP_034184641             399 aa            linear   INV 03-SEP-2025
DEFINITION  mitogen-activated protein kinase phosphatase 3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184641
VERSION     XP_034184641.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328750.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="mitogen-activated protein kinase phosphatase 3"
                     /calculated_mol_wt=43917
     Region          13..145
                     /region_name="DSP_MapKP"
                     /note="N-terminal regulatory rhodanese domain of dual
                     specificity phosphatases (DSP), such as Mapk Phosphatase.
                     This domain is believed to determine substrate specificity
                     by binding the substrate, such as ERK2, and activating the
                     C-terminal catalytic domain by...; cd01446"
                     /db_xref="CDD:238723"
     Site            59..61
                     /site_type="active"
                     /note="putative substrate binding residues [active]"
                     /db_xref="CDD:238723"
     Region          202..338
                     /region_name="DSP_MKP_classII"
                     /note="dual specificity phosphatase domain of class II
                     mitogen-activated protein kinase phosphatase; cd14566"
                     /db_xref="CDD:350414"
     CDS             1..399
                     /gene="Mkp3"
                     /coded_by="XM_034328750.2:1391..2590"
                     /db_xref="GeneID:117606393"
ORIGIN      
        1 mpggnvteee fvdpewlfre lrsrdgpskl lildcrahsd fseahirgsv plaipsimlr
       61 rlaagkvdll stircldlrn rvevflcgde nsrgtfvlig dstdpaghqg etiqvltrrl
      121 rssggcvail mngfeafrnr ypewcegsqt tgegppgqds ndgelmglrs lristpptrs
      181 ysdsdsdstc dsvgpeedkd fpveilphly lgnaansgdr ealarhriqy ilnvtpdlpn
      241 vfesagsmky mqipisdhws qnlasffpqa iqfieearss dkgvlvhcla gvsrsvtitv
      301 aylmhkcsls lndafnlvrs rksnvapnfh fmeqlhsfek elrdrgdrnr gndqrcigac
      361 rpggpcncpa psflspidlg lspdsgiefd rwasstpae
//
LOCUS       XP_034184643             123 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L31 [Osmia lignaria lignaria].
ACCESSION   XP_034184643
VERSION     XP_034184643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328752.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="ribosomal protein L31"
                     /calculated_mol_wt=14126
     Region          15..96
                     /region_name="Ribosomal_L31e"
                     /note="Ribosomal protein L31e; pfam01198"
                     /db_xref="CDD:460108"
     CDS             1..123
                     /gene="RpL31"
                     /coded_by="XM_034328752.1:131..502"
                     /db_xref="GeneID:117606394"
ORIGIN      
        1 makstekkgk sainevvtre ytvnlhkrlh svgfkkrapr aikeirkfae kqmgtpdvri
       61 dtrlnkqlws kgirnvpfrv rvrlsrrrnd dedsanklyt lvtyipvatf kglqtenvda
      121 sqd
//
LOCUS       XP_034184644             123 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L31 [Osmia lignaria lignaria].
ACCESSION   XP_034184644
VERSION     XP_034184644.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328753.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="ribosomal protein L31"
                     /calculated_mol_wt=14126
     Region          15..96
                     /region_name="Ribosomal_L31e"
                     /note="Ribosomal protein L31e; pfam01198"
                     /db_xref="CDD:460108"
     CDS             1..123
                     /gene="RpL31"
                     /coded_by="XM_034328753.2:113..484"
                     /db_xref="GeneID:117606394"
ORIGIN      
        1 makstekkgk sainevvtre ytvnlhkrlh svgfkkrapr aikeirkfae kqmgtpdvri
       61 dtrlnkqlws kgirnvpfrv rvrlsrrrnd dedsanklyt lvtyipvatf kglqtenvda
      121 sqd
//
LOCUS       XP_034190193            1200 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent RNA helicase pea [Osmia lignaria lignaria].
ACCESSION   XP_034190193
VERSION     XP_034190193.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334302.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1200
                     /product="ATP-dependent RNA helicase pea"
                     /calculated_mol_wt=136971
     Region          7..74
                     /region_name="GH2-like_DHX8"
                     /note="GIPC-homology 2 (GH2)-like domain found in DEAH box
                     protein 8 (DHX8) and similar proteins; cd21691"
                     /db_xref="CDD:409668"
     Region          247..325
                     /region_name="S1_DHX8_helicase"
                     /note="The N-terminal S1 domain of human ATP-dependent RNA
                     helicase DHX8, a DEAH (Asp-Glu-Ala-His) box polypeptide.
                     The DEAH-box RNA helicases are thought to play key roles
                     in pre-mRNA splicing and DHX8 facilitates nuclear export
                     of spliced mRNA by releasing...; cd05684"
                     /db_xref="CDD:240189"
     Site            order(255,263,276,278)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:240189"
     Region          536..>1161
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     CDS             1..1200
                     /gene="pea"
                     /coded_by="XM_034334302.2:216..3818"
                     /db_xref="GeneID:117608745"
ORIGIN      
        1 mdeveklehl slvskictel enhlglndkd laefiihlae knntfdkfkk vlvengaefs
       61 esfmanllri iqhmkpstqa lektlksask qdelaqkfpa lampneelpp vennelkddd
      121 ivnsvmasle elapskksgd skkdiqpsdn ndkkskhsrr srsrdkrrhr srssnrkdkr
      181 hrsrsrsrsr drsrsrdrrh rrsrdhrrsr srdrksrdhr dkhfgrhgsd rsrsrsveei
      241 sidpevgkiy sgkvanivpf gcfvqleglk rrweglvhis qlrregrvan asdvvsrgqk
      301 vlvkvlnigg qkvslsmkdv dqetgrdlnp vvavakpeed ekhlrnpdrp tsllelqgnw
      361 dedetysrkr vqrlsspekw eikqmlaasc idrnelpefd metgilpred deeedveiel
      421 veeeppflhg hgralgdlsp vrivknpdgs laqaammqsa lakerreqkm lqreqemdsv
      481 ptglnknwid plpeaesrtl aanmrgiglq tqdlpewkkh viggkkssfg kktnltlleq
      541 rqslpiyklr ddlvkavtdn qilivigetg sgkttqitqy laeagftarg kigctqprrv
      601 aamsvakrva eefgcrlgqe vgytirfedc tgpetnikym tdgmllrecl mdldlktysv
      661 imldeahert ihtdvlfgll kqavgrrpdl klivtsatld avkfsqyffe apiftipgrt
      721 fevevmytke petdyldaal itvmqihlre ppgdillflt gqeeidtace ilyermkslg
      781 pdvpeliilp vysalpsemq trifepappg srkvviatni aetsltidgi yyvvdpgfvk
      841 qkvynsktgm dslivtpisq aaakqragra grtgpgkcyr lyterayrde mlptpvpeiq
      901 rtnlattvlq lktmgindll hfdfmdappv eslimalesl hslsaldneg lltrlgrrma
      961 efplepnlsk mlimsvhlqc sdeiltivsm lsvqnvfyrp kdkqaladqk kakfnqpegd
     1021 hltllavyns wrnnkfsnaw cyenfvqirt lkraqdvrkq llgimdrhkl dvvsagkntv
     1081 riqkavcsgf frnaakkdpq egyrtlvdsq vvyihpssal fnrqpewviy helvqttkey
     1141 mrevttidpk wlvefapaff kfsdptklsk fkknqrlepl ynkyeepnaw risrvrrrrn
//
LOCUS       XP_034190195             714 aa            linear   INV 03-SEP-2025
DEFINITION  targeting protein for Xklp2 homolog isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190195
VERSION     XP_034190195.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334304.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190195.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..714
                     /product="targeting protein for Xklp2 homolog isoform X1"
                     /calculated_mol_wt=81733
     Region          305..>372
                     /region_name="TPX2_importin"
                     /note="Cell cycle regulated microtubule associated
                     protein; pfam12214"
                     /db_xref="CDD:463495"
     Region          <439..>679
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          623..696
                     /region_name="TPX2"
                     /note="Targeting protein for Xklp2 (TPX2) domain;
                     pfam06886"
                     /db_xref="CDD:462029"
     CDS             1..714
                     /gene="LOC117608746"
                     /coded_by="XM_034334304.2:269..2413"
                     /db_xref="GeneID:117608746"
ORIGIN      
        1 mmdnwcapqw adltrspqvl sdtyfevehe vheplitfkp nakssvpckk enitelsvae
       61 tnfddslepv ekpctsvayf iphegkkqsd deidkeknln namkdlklnk ktekihqaws
      121 ipapeltsaf kksavshkak paperfrina yttgvknqvs gksclknnqn idvhkkkent
      181 qhtalvkemd kpiielngds pkcvksnvsk erlsykivsg rrqsnvhktk krslskpypr
      241 vltcqyrrqs lkkyrrcsnq fismaeaisr fqngtpqrfr tisnknfqag smmklkqqtl
      301 klthpvsptl rskkrtrpit ilnqqerekl eleemkkhqi kanpvpinil kgpcvlkkvp
      361 kkpatvteef rltktryttg phlhsqsaid nrennckssl pitrstsasn vsikkdnast
      421 essgknikpl pigfvarnkq yqtkkeerlk nlqvqetnki knefharpap kftkpinpvk
      481 eqsekkrivp cpfsfekrdk slakkkeelv khkqkqnkei avfhanpapt fkpvlvhgls
      541 kesiqskekt aggtkklvsr qtkscfdqen kqpnvivsvn aikkgmkqca qefdkkqsan
      601 tvkvnnavnv ktekpkialr tkfelntekr akertefeek mrkkeeqlka kkeaeeknkm
      661 lreksertel rrmtevkarp mpiykpltil kstkppsspr spawasrnki rnvs
//
LOCUS       XP_034190196             714 aa            linear   INV 03-SEP-2025
DEFINITION  targeting protein for Xklp2 homolog isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190196
VERSION     XP_034190196.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334305.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190196.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..714
                     /product="targeting protein for Xklp2 homolog isoform X1"
                     /calculated_mol_wt=81733
     Region          305..>372
                     /region_name="TPX2_importin"
                     /note="Cell cycle regulated microtubule associated
                     protein; pfam12214"
                     /db_xref="CDD:463495"
     Region          <439..>679
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          623..696
                     /region_name="TPX2"
                     /note="Targeting protein for Xklp2 (TPX2) domain;
                     pfam06886"
                     /db_xref="CDD:462029"
     CDS             1..714
                     /gene="LOC117608746"
                     /coded_by="XM_034334305.2:105..2249"
                     /db_xref="GeneID:117608746"
ORIGIN      
        1 mmdnwcapqw adltrspqvl sdtyfevehe vheplitfkp nakssvpckk enitelsvae
       61 tnfddslepv ekpctsvayf iphegkkqsd deidkeknln namkdlklnk ktekihqaws
      121 ipapeltsaf kksavshkak paperfrina yttgvknqvs gksclknnqn idvhkkkent
      181 qhtalvkemd kpiielngds pkcvksnvsk erlsykivsg rrqsnvhktk krslskpypr
      241 vltcqyrrqs lkkyrrcsnq fismaeaisr fqngtpqrfr tisnknfqag smmklkqqtl
      301 klthpvsptl rskkrtrpit ilnqqerekl eleemkkhqi kanpvpinil kgpcvlkkvp
      361 kkpatvteef rltktryttg phlhsqsaid nrennckssl pitrstsasn vsikkdnast
      421 essgknikpl pigfvarnkq yqtkkeerlk nlqvqetnki knefharpap kftkpinpvk
      481 eqsekkrivp cpfsfekrdk slakkkeelv khkqkqnkei avfhanpapt fkpvlvhgls
      541 kesiqskekt aggtkklvsr qtkscfdqen kqpnvivsvn aikkgmkqca qefdkkqsan
      601 tvkvnnavnv ktekpkialr tkfelntekr akertefeek mrkkeeqlka kkeaeeknkm
      661 lreksertel rrmtevkarp mpiykpltil kstkppsspr spawasrnki rnvs
//
LOCUS       XP_034190197             616 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608746 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190197
VERSION     XP_034190197.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334306.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190197.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..616
                     /product="uncharacterized protein LOC117608746 isoform X2"
                     /calculated_mol_wt=70197
     Region          305..>372
                     /region_name="TPX2_importin"
                     /note="Cell cycle regulated microtubule associated
                     protein; pfam12214"
                     /db_xref="CDD:463495"
     CDS             1..616
                     /gene="LOC117608746"
                     /coded_by="XM_034334306.2:272..2122"
                     /db_xref="GeneID:117608746"
ORIGIN      
        1 mmdnwcapqw adltrspqvl sdtyfevehe vheplitfkp nakssvpckk enitelsvae
       61 tnfddslepv ekpctsvayf iphegkkqsd deidkeknln namkdlklnk ktekihqaws
      121 ipapeltsaf kksavshkak paperfrina yttgvknqvs gksclknnqn idvhkkkent
      181 qhtalvkemd kpiielngds pkcvksnvsk erlsykivsg rrqsnvhktk krslskpypr
      241 vltcqyrrqs lkkyrrcsnq fismaeaisr fqngtpqrfr tisnknfqag smmklkqqtl
      301 klthpvsptl rskkrtrpit ilnqqerekl eleemkkhqi kanpvpinil kgpcvlkkvp
      361 kkpatvteef rltktryttg phlhsqsaid nrennckssl pitrstsasn vsikkdnast
      421 essgknikpl pigfvarnkq yqtkkeerlk nlqvqetnki knefharpap kftkpinpvk
      481 eqsekkrivp cpfsfekrdk slakkkeelv khkqkqnkei avfhanpapt fkpvlvhgls
      541 kesiqskekt aggtkklvsr qtkscfdqen kqpnvivsvn aikkgmkqca qefdkkqsan
      601 tvkkkeqrse pslkkk
//
LOCUS       XP_034190198             431 aa            linear   INV 03-SEP-2025
DEFINITION  dnaJ homolog subfamily C member 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190198
VERSION     XP_034190198.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334307.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..431
                     /product="dnaJ homolog subfamily C member 1"
                     /calculated_mol_wt=50164
     Region          41..102
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(69..71,79,82..83,86..87)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          294..339
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(294,326..327,329..330,332..334,336..338)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          374..421
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(374,408..409,411..412,414..416,418..420)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     CDS             1..431
                     /gene="LOC117608747"
                     /coded_by="XM_034334307.2:1..1296"
                     /db_xref="GeneID:117608747"
ORIGIN      
        1 mrvslllifr llyffnvyqf tnawdneele vfdvveevnq nfyevlgvpq aanaseikka
       61 frrlslqlhp dknpaedaeq qfrklvavyd vlkdpgkrqk ydnvlvnglp nwrsavyyyr
      121 hvrkmgllel siilfliitv gqyvvawgay fekrytyeqv lgsklqkmqk knkkgkmdvp
      181 dladilekip tptiwntlpf qfprwiiaft lsipdvvrvm yslleerkrr kkleeeeata
      241 qeteqlppep tsrarrrraa ftpqersgnn ikeasrkdqt shvyqkpalf gglwtdddil
      301 eliklvkkyp sgtperwdki adamnrtvae vthmakkikd eglkpgaste evvieerprk
      361 tktraesvdn vsdwsqeqqr aleaalikyp kgmsvdrwek iancvegktk decqaryrql
      421 velikkkqet q
//
LOCUS       XP_034190199             402 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190199
VERSION     XP_034190199.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334308.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X1"
                     /calculated_mol_wt=46441
     Region          46..327
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          307..>402
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..402
                     /gene="LOC117608749"
                     /coded_by="XM_034334308.2:552..1760"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mesaitgrrr ttarhsaedq aldqiakeae arlaarrqar aeareirmre lerqqkeaee
       61 nadrvydmca advnrtmrvt pdsvrtsrll tnsnnfqssr rssedsleda glsrdlrlel
      121 kefeekfrka mianaqldne kssyayqvdl lkdkleelee tvaqlrrelr eknreseqlk
      181 rvsqrlkdel dichaqller dtliqenglv iiddeesene dndiengprp rrrvlvstea
      241 aellqnagkg sldvrlrkfa sekkelqdei rhlrleleet rnrirseksp gsvlgclses
      301 edvqreankl ladykfklqk aeqdmstlqa tvarlesqvi ryksaaease kaedelkvek
      361 rklqrevret qgrveeleta nshlqrrldk lknaksallk el
//
LOCUS       XP_034190200             402 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190200
VERSION     XP_034190200.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334309.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X1"
                     /calculated_mol_wt=46441
     Region          46..327
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          307..>402
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..402
                     /gene="LOC117608749"
                     /coded_by="XM_034334309.2:536..1744"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mesaitgrrr ttarhsaedq aldqiakeae arlaarrqar aeareirmre lerqqkeaee
       61 nadrvydmca advnrtmrvt pdsvrtsrll tnsnnfqssr rssedsleda glsrdlrlel
      121 kefeekfrka mianaqldne kssyayqvdl lkdkleelee tvaqlrrelr eknreseqlk
      181 rvsqrlkdel dichaqller dtliqenglv iiddeesene dndiengprp rrrvlvstea
      241 aellqnagkg sldvrlrkfa sekkelqdei rhlrleleet rnrirseksp gsvlgclses
      301 edvqreankl ladykfklqk aeqdmstlqa tvarlesqvi ryksaaease kaedelkvek
      361 rklqrevret qgrveeleta nshlqrrldk lknaksallk el
//
LOCUS       XP_034190201             402 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190201
VERSION     XP_034190201.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334310.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X1"
                     /calculated_mol_wt=46441
     Region          46..327
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          307..>402
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..402
                     /gene="LOC117608749"
                     /coded_by="XM_034334310.2:251..1459"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mesaitgrrr ttarhsaedq aldqiakeae arlaarrqar aeareirmre lerqqkeaee
       61 nadrvydmca advnrtmrvt pdsvrtsrll tnsnnfqssr rssedsleda glsrdlrlel
      121 kefeekfrka mianaqldne kssyayqvdl lkdkleelee tvaqlrrelr eknreseqlk
      181 rvsqrlkdel dichaqller dtliqenglv iiddeesene dndiengprp rrrvlvstea
      241 aellqnagkg sldvrlrkfa sekkelqdei rhlrleleet rnrirseksp gsvlgclses
      301 edvqreankl ladykfklqk aeqdmstlqa tvarlesqvi ryksaaease kaedelkvek
      361 rklqrevret qgrveeleta nshlqrrldk lknaksallk el
//
LOCUS       XP_034190202             402 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190202
VERSION     XP_034190202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X1"
                     /calculated_mol_wt=46441
     Region          46..327
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          307..>402
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..402
                     /gene="LOC117608749"
                     /coded_by="XM_034334311.2:239..1447"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mesaitgrrr ttarhsaedq aldqiakeae arlaarrqar aeareirmre lerqqkeaee
       61 nadrvydmca advnrtmrvt pdsvrtsrll tnsnnfqssr rssedsleda glsrdlrlel
      121 kefeekfrka mianaqldne kssyayqvdl lkdkleelee tvaqlrrelr eknreseqlk
      181 rvsqrlkdel dichaqller dtliqenglv iiddeesene dndiengprp rrrvlvstea
      241 aellqnagkg sldvrlrkfa sekkelqdei rhlrleleet rnrirseksp gsvlgclses
      301 edvqreankl ladykfklqk aeqdmstlqa tvarlesqvi ryksaaease kaedelkvek
      361 rklqrevret qgrveeleta nshlqrrldk lknaksallk el
//
LOCUS       XP_034190203             355 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190203
VERSION     XP_034190203.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334312.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X2"
                     /calculated_mol_wt=41140
     Region          1..280
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          260..>355
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..355
                     /gene="LOC117608749"
                     /coded_by="XM_034334312.2:256..1323"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mrelerqqke aeenadrvyd mcaadvnrtm rvtpdsvrts rlltnsnnfq ssrrssedsl
       61 edaglsrdlr lelkefeekf rkamianaql dnekssyayq vdllkdklee leetvaqlrr
      121 elreknrese qlkrvsqrlk deldichaql lerdtliqen glviiddees enedndieng
      181 prprrrvlvs teaaellqna gkgsldvrlr kfasekkelq deirhlrlel eetrnrirse
      241 kspgsvlgcl sesedvqrea nklladykfk lqkaeqdmst lqatvarles qviryksaae
      301 asekaedelk vekrklqrev retqgrveel etanshlqrr ldklknaksa llkel
//
LOCUS       XP_034190204             355 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190204
VERSION     XP_034190204.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334313.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X2"
                     /calculated_mol_wt=41140
     Region          1..280
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          260..>355
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..355
                     /gene="LOC117608749"
                     /coded_by="XM_034334313.2:504..1571"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mrelerqqke aeenadrvyd mcaadvnrtm rvtpdsvrts rlltnsnnfq ssrrssedsl
       61 edaglsrdlr lelkefeekf rkamianaql dnekssyayq vdllkdklee leetvaqlrr
      121 elreknrese qlkrvsqrlk deldichaql lerdtliqen glviiddees enedndieng
      181 prprrrvlvs teaaellqna gkgsldvrlr kfasekkelq deirhlrlel eetrnrirse
      241 kspgsvlgcl sesedvqrea nklladykfk lqkaeqdmst lqatvarles qviryksaae
      301 asekaedelk vekrklqrev retqgrveel etanshlqrr ldklknaksa llkel
//
LOCUS       XP_034190205             355 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190205
VERSION     XP_034190205.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334314.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X2"
                     /calculated_mol_wt=41140
     Region          1..280
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          260..>355
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..355
                     /gene="LOC117608749"
                     /coded_by="XM_034334314.2:146..1213"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mrelerqqke aeenadrvyd mcaadvnrtm rvtpdsvrts rlltnsnnfq ssrrssedsl
       61 edaglsrdlr lelkefeekf rkamianaql dnekssyayq vdllkdklee leetvaqlrr
      121 elreknrese qlkrvsqrlk deldichaql lerdtliqen glviiddees enedndieng
      181 prprrrvlvs teaaellqna gkgsldvrlr kfasekkelq deirhlrlel eetrnrirse
      241 kspgsvlgcl sesedvqrea nklladykfk lqkaeqdmst lqatvarles qviryksaae
      301 asekaedelk vekrklqrev retqgrveel etanshlqrr ldklknaksa llkel
//
LOCUS       XP_034190206             355 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat flightless-interacting protein 2 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190206
VERSION     XP_034190206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334315.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135039) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="8"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="leucine-rich repeat flightless-interacting
                     protein 2 isoform X2"
                     /calculated_mol_wt=41140
     Region          1..280
                     /region_name="LRRFIP"
                     /note="LRRFIP family; pfam09738"
                     /db_xref="CDD:462869"
     Region          260..>355
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..355
                     /gene="LOC117608749"
                     /coded_by="XM_034334315.2:307..1374"
                     /db_xref="GeneID:117608749"
ORIGIN      
        1 mrelerqqke aeenadrvyd mcaadvnrtm rvtpdsvrts rlltnsnnfq ssrrssedsl
       61 edaglsrdlr lelkefeekf rkamianaql dnekssyayq vdllkdklee leetvaqlrr
      121 elreknrese qlkrvsqrlk deldichaql lerdtliqen glviiddees enedndieng
      181 prprrrvlvs teaaellqna gkgsldvrlr kfasekkelq deirhlrlel eetrnrirse
      241 kspgsvlgcl sesedvqrea nklladykfk lqkaeqdmst lqatvarles qviryksaae
      301 asekaedelk vekrklqrev retqgrveel etanshlqrr ldklknaksa llkel
//
LOCUS       XP_034189080            1338 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein 164 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189080
VERSION     XP_034189080.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333189.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189080.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1338
                     /product="centrosomal protein 164 isoform X1"
                     /calculated_mol_wt=153502
     Region          57..87
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <571..>823
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          610..>1140
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1338
                     /gene="Cep164"
                     /coded_by="XM_034333189.2:304..4320"
                     /db_xref="GeneID:117608123"
ORIGIN      
        1 msisqdsaat ilgrevfdet shpsneevld yarrlgidpd aephllvlar eglmaalpkg
       61 wkpcyhease swyyyqastg tttwehpldp vykqlvkqar agntrqmsve edskttakdl
      121 esheeatlpk etssketikh tfkmnpsttr iptklaplkk ldklegvrrk dsssqdrqhs
      181 rrdsdskten platdrasrd ytnlrfqdpk fyecpkvlea mapiaatvta atttitsptl
      241 eldlkevlkr seslsprhek dweqlsskfs seeniididk lsittlarse rsekfdkekh
      301 psqfsqqkel tlsgggtmfl ksnrsrdttp sqeggklddf rtvaisdegc nivgdrlksi
      361 lrekqseddd rpvdeerksv rfdiekevdi kftysgsedd wdseseeqnv risavvsnka
      421 tgsilfsqrs ssqslddenk edsesklded trkssssetp rnskvvgkrf vvqnvteneh
      481 rtqmakenlr rdslsressl dyvpsnkfmk iknidlvsrs etdcsvvkss dsedirkskp
      541 ktvkpkksai rfskdrqtdd ddtsdlsrvn qdleqsrrer lkeehsknld etrtglskdh
      601 qrdiemlkke fedkleqtkk eleenfveqk qqlqknlser leelkremae keeqeiqkli
      661 aemdkarlen lkkvkaelev cyekerqeil anlktelder rkellelrnq emgklenehe
      721 rdlgeeklak lnefeltkqh sekiealkke lekefddlrt elriqqreki tkitedhekc
      781 laeilrdfri dedltrkmyk qrleeihadf srdaekeark qteralqqds iefekmrcek
      841 rllldkymal kekymklkne vrlaverrsr rkegyttase terststrtr tdktesseqn
      901 tplrntrpsl attsakaqet aiakeqteeq hkpdesnvqs vqkvqnsgfq kgaaigaaai
      961 pktaavries ddtttasetn anmlmkkkkn fskkatstar astagnnnnn nlenpvenir
     1021 kqlekledlg dqlpsnetay tlrypfqdka lantsselef frhrihverd svrrarealr
     1081 hqenafhgrq kawkqrsara tleqlvqeer elsemevslh rtrsllgekv ihlrhleqsl
     1141 drvasakrne neatstrnde ltlsdmssas sgfsstdlgt dtfidkpdhy qesteiiasl
     1201 eslnseirei wgvlnkrldt nasppptfmy sylrwlrfhh laaqsnsvqg lenfpsgtfg
     1261 tpniqsnils qltatqpptt ttqniiaqyg pnsgfttsvg tvdkpisnlm ertwnlrdwl
     1321 rqacaentdl ispgqatl
//
LOCUS       XP_034189978            1331 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein 164 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034189978
VERSION     XP_034189978.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334087.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189978.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1331
                     /product="centrosomal protein 164 isoform X2"
                     /calculated_mol_wt=152757
     Region          57..87
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <571..>823
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          610..>1140
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1331
                     /gene="Cep164"
                     /coded_by="XM_034334087.2:303..4298"
                     /db_xref="GeneID:117608123"
ORIGIN      
        1 msisqdsaat ilgrevfdet shpsneevld yarrlgidpd aephllvlar eglmaalpkg
       61 wkpcyhease swyyyqastg tttwehpldp vykqlvkqar agntrqmsve edskttakdl
      121 esheeatlpk etssketikh tfkmnpsttr iptklaplkk ldklegvrrk dsssqdrqhs
      181 rrdsdskten platdrasrd ytnlrfqdpk fyecpkvlea mapiaatvta atttitsptl
      241 eldlkevlkr seslsprhek dweqlsskfs seeniididk lsittlarse rsekfdkekh
      301 psqfsqqkel tlsgggtmfl ksnrsrdttp sqeggklddf rtvaisdegc nivgdrlksi
      361 lrekqseddd rpvdeerksv rfdiekevdi kftysgsedd wdseseeqnv risavvsnka
      421 tgsilfsqrs ssqslddenk edsesklded trkssssetp rnskvvgkrf vvqnvteneh
      481 rtqmakenlr rdslsressl dyvpsnkfmk iknidlvsrs etdcsvvkss dsedirkskp
      541 ktvkpkksai rfskdrqtdd ddtsdlsrvn qdleqsrrer lkeehsknld etrtglskdh
      601 qrdiemlkke fedkleqtkk eleenfveqk qqlqknlser leelkremae keeqeiqkli
      661 aemdkarlen lkkvkaelev cyekerqeil anlktelder rkellelrnq emgklenehe
      721 rdlgeeklak lnefeltkqh sekiealkke lekefddlrt elriqqreki tkitedhekc
      781 laeilrdfri dedltrkmyk qrleeihadf srdaekeark qteralqqds iefekmrcek
      841 rllldkymal kekymklkne vrlaverrsr rkegyttase terststrtr tdktesseqn
      901 tplrntrpsl attsakaqet aiakeqteeq hkpdesnvqs vqkvqnsgfq kgaaigaaai
      961 pktaavries ddtttasetn anmlmkkkkn fskkatstar astagnnnnn nlenpvenir
     1021 kqlekledlg dqlpsnetay tlrypfqdka lantsselef frhrihverd svrrarealr
     1081 hqenafhgrq kawkqrsara tleqlvqeer elsemevslh rtrsllgekv ihlrhleqsl
     1141 drvasakrne neatstrnde ltlsdmssas sgfsstdlgt dtfidkpdhy qesteiiasl
     1201 eslnseirei wgvlnkrldt nasppptfmy sylrwlrfhh laaqsnsvqg tfgtpniqsn
     1261 ilsqltatqp pttttqniia qygpnsgftt svgtvdkpis nlmertwnlr dwlrqacaen
     1321 tdlispgqat l
//
LOCUS       XP_034190808            1195 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein 164 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190808
VERSION     XP_034190808.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334917.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190808.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1195
                     /product="centrosomal protein 164 isoform X3"
                     /calculated_mol_wt=137381
     Region          <428..>680
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          476..>997
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1195
                     /gene="Cep164"
                     /coded_by="XM_034334917.2:874..4461"
                     /db_xref="GeneID:117608123"
ORIGIN      
        1 mnpsttript klaplkkldk legvrrkdss sqdrqhsrrd sdsktenpla tdrasrdytn
       61 lrfqdpkfye cpkvleamap iaatvtaatt titsptleld lkevlkrses lsprhekdwe
      121 qlsskfssee niididklsi ttlarserse kfdkekhpsq fsqqkeltls gggtmflksn
      181 rsrdttpsqe ggklddfrtv aisdegcniv gdrlksilre kqsedddrpv deerksvrfd
      241 iekevdikft ysgseddwds eseeqnvris avvsnkatgs ilfsqrsssq slddenkeds
      301 eskldedtrk ssssetprns kvvgkrfvvq nvtenehrtq makenlrrds lsressldyv
      361 psnkfmkikn idlvsrsetd csvvkssdse dirkskpktv kpkksairfs kdrqtddddt
      421 sdlsrvnqdl eqsrrerlke ehsknldetr tglskdhqrd iemlkkefed kleqtkkele
      481 enfveqkqql qknlserlee lkremaekee qeiqkliaem dkarlenlkk vkaelevcye
      541 kerqeilanl ktelderrke llelrnqemg kleneherdl geeklaklne feltkqhsek
      601 iealkkelek efddlrtelr iqqrekitki tedhekclae ilrdfrided ltrkmykqrl
      661 eeihadfsrd aekearkqte ralqqdsief ekmrcekrll ldkymalkek ymklknevrl
      721 averrsrrke gyttaseter ststrtrtdk tesseqntpl rntrpslatt sakaqetaia
      781 keqteeqhkp desnvqsvqk vqnsgfqkga aigaaaipkt aavriesddt ttasetnanm
      841 lmkkkknfsk katstarast agnnnnnnle npvenirkql ekledlgdql psnetaytlr
      901 ypfqdkalan tsseleffrh rihverdsvr rarealrhqe nafhgrqkaw kqrsaratle
      961 qlvqeerels emevslhrtr sllgekvihl rhleqsldrv asakrnenea tstrndeltl
     1021 sdmssassgf sstdlgtdtf idkpdhyqes teiiaslesl nseireiwgv lnkrldtnas
     1081 ppptfmysyl rwlrfhhlaa qsnsvqglen fpsgtfgtpn iqsnilsqlt atqppttttq
     1141 niiaqygpns gfttsvgtvd kpisnlmert wnlrdwlrqa caentdlisp gqatl
//
LOCUS       XP_034191660            1124 aa            linear   INV 03-SEP-2025
DEFINITION  centrosomal protein 164 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034191660
VERSION     XP_034191660.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191660.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1124
                     /product="centrosomal protein 164 isoform X4"
                     /calculated_mol_wt=130002
     Region          57..87
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <571..>823
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     CDS             1..1124
                     /gene="Cep164"
                     /coded_by="XM_034335769.2:304..3678"
                     /db_xref="GeneID:117608123"
ORIGIN      
        1 msisqdsaat ilgrevfdet shpsneevld yarrlgidpd aephllvlar eglmaalpkg
       61 wkpcyhease swyyyqastg tttwehpldp vykqlvkqar agntrqmsve edskttakdl
      121 esheeatlpk etssketikh tfkmnpsttr iptklaplkk ldklegvrrk dsssqdrqhs
      181 rrdsdskten platdrasrd ytnlrfqdpk fyecpkvlea mapiaatvta atttitsptl
      241 eldlkevlkr seslsprhek dweqlsskfs seeniididk lsittlarse rsekfdkekh
      301 psqfsqqkel tlsgggtmfl ksnrsrdttp sqeggklddf rtvaisdegc nivgdrlksi
      361 lrekqseddd rpvdeerksv rfdiekevdi kftysgsedd wdseseeqnv risavvsnka
      421 tgsilfsqrs ssqslddenk edsesklded trkssssetp rnskvvgkrf vvqnvteneh
      481 rtqmakenlr rdslsressl dyvpsnkfmk iknidlvsrs etdcsvvkss dsedirkskp
      541 ktvkpkksai rfskdrqtdd ddtsdlsrvn qdleqsrrer lkeehsknld etrtglskdh
      601 qrdiemlkke fedkleqtkk eleenfveqk qqlqknlser leelkremae keeqeiqkli
      661 aemdkarlen lkkvkaelev cyekerqeil anlktelder rkellelrnq emgklenehe
      721 rdlgeeklak lnefeltkqh sekiealkke lekefddlrt elriqqreki tkitedhekc
      781 laeilrdfri dedltrkmyk qrleeihadf srdaekeark qteralqqds iefekmrcek
      841 rllldkymal kekymklkne vrlaverrsr rkegyttase terststrtr tdktesseqn
      901 tplrntrpsl attsakaqet aiakeqteeq hkpdesnvqs vqkvqnsgfq kgaaigaaai
      961 pktaavries ddtttasetn anmlmkkkkn fskkatstar astagnnnnn nlenpvenir
     1021 kqlekledlg dqlpsnetay tlrypfqdka lantsselef frhrihverd svrrarealr
     1081 hqenafhgrq kawkqrsara tleqlvqilc kffsrrnenf rkwr
//
LOCUS       XP_034192784             587 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 12a5, mitochondrial isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192784
VERSION     XP_034192784.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192784.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..587
                     /product="putative cytochrome P450 12a5, mitochondrial
                     isoform X1"
                     /calculated_mol_wt=67600
     Region          152..580
                     /region_name="CYP24A1-like"
                     /note="cytochrome P450 family 24 subfamily A, polypeptide
                     1 and similar cytochrome P450s; cd11054"
                     /db_xref="CDD:410677"
     Site            order(191,197,213,268..269,272,310,312..313,341,394,
                     397..398,401..402,463..468,571..572)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410677"
     Site            order(191,212..213,220,224,395,398..399,402..403,406,458,
                     463..464,467,469,527..530,533..537,541)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410677"
     CDS             1..587
                     /gene="LOC117609814"
                     /coded_by="XM_034336893.2:170..1933"
                     /db_xref="GeneID:117609814"
ORIGIN      
        1 mqrllpkfts isrrcsvmkl skplatndsq kcldlnasdi lsapitqsqt tsvmnesltr
       61 tnsiplldqs aaevstatfe ittsknesqi igdrsaqpfe eipgpavlki wekywkfvpl
      121 fgtqlfssll inrftqgrls wnrnvtplky lfneygcivr ingplsgdiv mihrpehiae
      181 vfkqegdtpl rsgidilqhy rlnyrkyrla gafslqgsew lelrekveqe feqiasnffd
      241 ridaicdeli nriykirnrq nevpatfhed llrwgmecfc nltfnkhlgf lesagynsts
      301 etsrivnamm vahkymsrce tgfqvwrffl tpfarklfda cdvldgvigk yvrhaqnklr
      361 srspqdvegg spvlekflln egirpddlct llmdmmilgv qatvnseafl lyylarnprt
      421 qrkiydeivs vlpssdspft ektvrnmpyl kaclqeslrl rpavpyitrl lpktislhgy
      481 tipkgtfvim anqitsqree nfedpekfrp erwlnskked tdfsylpfgy garsclgknm
      541 aeakmmllta klirqfriey dyadikssfm mvnvpnkplr frfvdrn
//
LOCUS       XP_034193629             572 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 12a5, mitochondrial isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034193629
VERSION     XP_034193629.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337738.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193629.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="putative cytochrome P450 12a5, mitochondrial
                     isoform X2"
                     /calculated_mol_wt=65893
     Region          137..565
                     /region_name="CYP24A1-like"
                     /note="cytochrome P450 family 24 subfamily A, polypeptide
                     1 and similar cytochrome P450s; cd11054"
                     /db_xref="CDD:410677"
     Site            order(176,182,198,253..254,257,295,297..298,326,379,
                     382..383,386..387,448..453,556..557)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410677"
     Site            order(176,197..198,205,209,380,383..384,387..388,391,443,
                     448..449,452,454,512..515,518..522,526)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410677"
     CDS             1..572
                     /gene="LOC117609814"
                     /coded_by="XM_034337738.2:170..1888"
                     /db_xref="GeneID:117609814"
ORIGIN      
        1 mqrllpkfts isrrcsvmkl skplatndsq kcldlnasdi lsapitqsqt tsvmnesltr
       61 tnsiplldqs aaevstatfe ittsknesqi igdrsaqpfe eipgpavlki wekywkfvpl
      121 fgrlswnrnv tplkylfney gcivringpl sgdivmihrp ehiaevfkqe gdtplrsgid
      181 ilqhyrlnyr kyrlagafsl qgsewlelre kveqefeqia snffdridai cdelinriyk
      241 irnrqnevpa tfhedllrwg mecfcnltfn khlgflesag ynstsetsri vnammvahky
      301 msrcetgfqv wrffltpfar klfdacdvld gvigkyvrha qnklrsrspq dveggspvle
      361 kfllnegirp ddlctllmdm milgvqatvn seafllyyla rnprtqrkiy deivsvlpss
      421 dspftektvr nmpylkaclq eslrlrpavp yitrllpkti slhgytipkg tfvimanqit
      481 sqreenfedp ekfrperwln skkedtdfsy lpfgygarsc lgknmaeakm mlltaklirq
      541 frieydyadi kssfmmvnvp nkplrfrfvd rn
//
LOCUS       XP_034195595             373 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor 49b-like [Osmia lignaria lignaria].
ACCESSION   XP_034195595
VERSION     XP_034195595.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339704.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="odorant receptor 49b-like"
                     /calculated_mol_wt=42017
     Region          64..361
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..373
                     /gene="LOC117611589"
                     /coded_by="XM_034339704.2:53..1174"
                     /db_xref="GeneID:117611589"
ORIGIN      
        1 mirnlrevln ilelagtisc twpldpntkr wkkvaynirw tfafidivvv silllikmyy
       61 irhdiveltk aisllsaitd gtlnllfckl nasklqvlia kvrqhldran edenkqiqvy
      121 vdrykvvlsv vgmsyiings sfnflpfytd qvlpiecwyp fsietpalyc vayvleaccv
      181 vptalaifid ykvmsllfyi aarlnvlgek lkqvdnyeql ancieehqei lgfledtrla
      241 vkallfkvvv tmgatvisag fpllymddpm sdlqtvyslg sfvvigsghv yllaapaedl
      301 kesgsqfats vteiqwigqs kgfgtsvvim mqrsqkpcii plglipglsm eyftqfltti
      361 ysyfmamrtm iea
//
LOCUS       XP_034195514             373 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor 49b-like [Osmia lignaria lignaria].
ACCESSION   XP_034195514
VERSION     XP_034195514.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339623.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="odorant receptor 49b-like"
                     /calculated_mol_wt=42017
     Region          64..361
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..373
                     /gene="LOC117611589"
                     /coded_by="XM_034339623.2:53..1174"
                     /db_xref="GeneID:117611589"
ORIGIN      
        1 mirnlrevln ilelagtisc twpldpntkr wkkvaynirw tfafidivvv silllikmyy
       61 irhdiveltk aisllsaitd gtlnllfckl nasklqvlia kvrqhldran edenkqiqvy
      121 vdrykvvlsv vgmsyiings sfnflpfytd qvlpiecwyp fsietpalyc vayvleaccv
      181 vptalaifid ykvmsllfyi aarlnvlgek lkqvdnyeql ancieehqei lgfledtrla
      241 vkallfkvvv tmgatvisag fpllymddpm sdlqtvyslg sfvvigsghv yllaapaedl
      301 kesgsqfats vteiqwigqs kgfgtsvvim mqrsqkpcii plglipglsm eyftqfltti
      361 ysyfmamrtm iea
//
LOCUS       XP_034195733             373 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor 13a-like [Osmia lignaria lignaria].
ACCESSION   XP_034195733
VERSION     XP_034195733.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339842.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195733.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="odorant receptor 13a-like"
                     /calculated_mol_wt=42498
     Region          64..361
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..373
                     /gene="LOC117611698"
                     /coded_by="XM_034339842.2:28..1149"
                     /db_xref="GeneID:117611698"
ORIGIN      
        1 mianlreild vielvgtilc twpidpnask cksillniyw mfavfsvgst ilcllittyy
       61 frhdlilltk ttcfisalse tlldliickm sskklqvlie kvrkyldttg kdennmiqvy
      121 vkryktffst mavayfingv sfffmplytn qhlpleswlp fstdtvmkyg ivyiiqayss
      181 mhtalsifvd fkiisllcft aarldilnak lkqgnsyemm vnyikehqei lrfledtkaa
      241 vqallfktni tmgsvviaaa fpllymknpm sepqvvyqfv cmvlagcghv ylisapsddl
      301 kessiqfams vtdiqwigkq knmttsvlim lqrsqkpcli plglipplsm eyfsqfltti
      361 ysyfmairtm ies
//
LOCUS       XP_076545974             298 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305628 [Osmia lignaria lignaria].
ACCESSION   XP_076545974
VERSION     XP_076545974.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689859.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="uncharacterized protein LOC143305628"
                     /calculated_mol_wt=34237
     Region          77..>280
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..298
                     /gene="LOC143305628"
                     /coded_by="XM_076689859.1:234..1130"
                     /db_xref="GeneID:143305628"
ORIGIN      
        1 meavctqngq yinmilnlrk vfdllelagr lsctwpedss tskwanvfrn vrwiftlism
       61 ivllislalg vyhhrnnflf lmktmseisa iinvifdlil sklhstelqt liqtirkhle
      121 taseyenhvi eryldrykef ytalvaayia sgifftvapl flsqnlpmdg wipfstesfa
      181 iycivyfves ycilqaalif vinvmivilf cfiaarldil gtklnqvncr ellvicvkeh
      241 qeiikffddt edavqgllfk mnvligmsvi satfpmlyvs laspinycyc ewiifdii
//
LOCUS       XP_076545975             269 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor Or2 [Osmia lignaria lignaria].
ACCESSION   XP_076545975
VERSION     XP_076545975.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689860.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="odorant receptor Or2"
                     /calculated_mol_wt=30893
     Region          1..242
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..269
                     /gene="LOC143305629"
                     /coded_by="XM_076689860.1:1..810"
                     /db_xref="GeneID:143305629"
ORIGIN      
        1 meefckratp serivlqryv drykyihgvy tlwcflttvf visgplyspq tfpthaiypf
       61 sverqplkcf iflhqslvgf qvssgmatdt qvalllryaa arsellgmql haaksgreln
      121 acikehsell rytrkvcesv rflvlatvat tiiavifgsl nlvtdqplpl kilyamivfs
      181 asvelfmyaw padnlmrmsa riatdayntn wfesdlamqr kiyfiirrsq kleiiringi
      241 vprlsllyya kylykstsyf aalriivek
//
LOCUS       XP_034195869             232 aa            linear   INV 03-SEP-2025
DEFINITION  H2.0-like homeobox protein [Osmia lignaria lignaria].
ACCESSION   XP_034195869
VERSION     XP_034195869.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339978.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..232
                     /product="H2.0-like homeobox protein"
                     /calculated_mol_wt=26221
     Region          124..184
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..232
                     /gene="LOC117611744"
                     /coded_by="XM_034339978.2:189..887"
                     /db_xref="GeneID:117611744"
ORIGIN      
        1 mnevntrmvd dtncskqlkf gverilsdef stkrtdilrp lpvqfstlrc pcpgacvrce
       61 alrlcptfty aqtaingnyg sgnhgsgive nypniyrptp lrpvprvsis sastptsqse
      121 sntrrkrsws ravfsslqrk glerrfslqk yitkpdrrql aatlgltdaq vkvwfqnrrm
      181 kwrhtkeses ssltnpdsqk essrklikes aknantektv sddedieiev dv
//
LOCUS       XP_076545976             198 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305228 [Osmia lignaria lignaria].
ACCESSION   XP_076545976
VERSION     XP_076545976.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689861.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..198
                     /product="uncharacterized protein LOC143305228"
                     /calculated_mol_wt=22612
     CDS             1..198
                     /gene="LOC143305228"
                     /coded_by="XM_076689861.1:423..1019"
                     /db_xref="GeneID:143305228"
ORIGIN      
        1 mtkrnfspii giftalllfy lktisanrts fyleevrsls elpmtelihi ksslideedf
       61 ttddeheeea ssrtlsvslp vaqplkrmnk ksirryedhy dekgkdskis kifqlsvtal
      121 sflafggyll tliittirqn sgtngngnvi vlsnlqglqn ynrprrnvri ldpaenefem
      181 eklyrgmiml srtyaayn
//
LOCUS       XP_034194733              85 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor 46a-like [Osmia lignaria lignaria].
ACCESSION   XP_034194733
VERSION     XP_034194733.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338842.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194733.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..85
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..85
                     /product="odorant receptor 46a-like"
                     /calculated_mol_wt=9810
     Region          <8..58
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..85
                     /gene="LOC117610772"
                     /coded_by="XM_034338842.2:11..268"
                     /db_xref="GeneID:117610772"
ORIGIN      
        1 myilsraadd lressirfsi sinniqwigk srkmtnivli mmqrcqkpfl vsmggffpal
       61 sleyysqflt tissyfmamr ttmnf
//
LOCUS       XP_034196577             376 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612021 [Osmia lignaria lignaria].
ACCESSION   XP_034196577
VERSION     XP_034196577.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340686.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196577.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="uncharacterized protein LOC117612021"
                     /calculated_mol_wt=43318
     Region          70..346
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..376
                     /gene="LOC117612021"
                     /coded_by="XM_034340686.2:68..1198"
                     /db_xref="GeneID:117612021"
ORIGIN      
        1 mlknitpeka iaftrysval tccwplfsha trfqlirfri srsvvllnaf llflpliysl
       61 yinyvhndhv gfsksiclfi avsqvllntf vcivqqdrfw elivemkmcc neakpyerqv
      121 yqtyvdkyst fyavsaiwfy ltasiivigt lfipqpfptn aeypfpvdyq plksilflhq
      181 sflvfqcsah vcinmlcall mlfaaarfei lkmelrevks fnelvdcvkk yyiarryakk
      241 vssasrfial itvimcgtvl vfcgvnfier qpftviiqfm slagtgliev fmvawpadhl
      301 ldmsensvrg iyesrwylqs tsmqknilim llpqtpvaik ilyiiptlsl nyycsfvsnv
      361 lslftvlrat iqnddi
//
LOCUS       XP_034196705             525 aa            linear   INV 03-SEP-2025
DEFINITION  target of Myb1 membrane trafficking protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196705
VERSION     XP_034196705.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340814.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="target of Myb1 membrane trafficking protein
                     isoform X1"
                     /calculated_mol_wt=58204
     Region          14..151
                     /region_name="VHS_Tom1_like"
                     /note="VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily;
                     cd03565"
                     /db_xref="CDD:340768"
     Site            order(32..33,36,40,83..84)
                     /site_type="other"
                     /note="putative intraprotein/interprotein binding site
                     [polypeptide binding]"
                     /db_xref="CDD:340768"
     Site            order(50,54,57..58)
                     /site_type="other"
                     /note="putative membrane binding residues"
                     /db_xref="CDD:340768"
     Region          216..308
                     /region_name="GAT_TOM1_like"
                     /note="canonical GAT domain found in target of myb protein
                     1 (Tom1) protein family; cd14233"
                     /db_xref="CDD:410581"
     Site            order(224..225,228..229,231..232,235..236,245,258,
                     261..262)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:410581"
     CDS             1..525
                     /gene="LOC117612087"
                     /coded_by="XM_034340814.2:248..1825"
                     /db_xref="GeneID:117612087"
ORIGIN      
        1 msffgvnvnp fstpvgqkie qatdgslpse nwtlnmeicd iinetedgpr daikaikrrl
       61 nqaagknyti vmytltvlet cvkncgkrfh tlacsrefiq elvkligpkn epptavqekv
      121 lsliqtwadt frhqphtqgv vqvyqelkvk giqfpmtdld amapiitper svpeseqsii
      181 nvptteqqsi tpatlqvqqp pnqssgqliq lneqqmaklq seldvvqgnm rvlsemlaff
      241 tssdqnnsqq pdpadfellt elhstckamq ervvdligkl ahdemtaell rindelnnlf
      301 lrysrytknk avaastilaq tighpqnmds assnkkeepd slidlsdetd tlekkvtgmd
      361 iadnadknri erkekkegdn defdmfaqsr nttyettkns gskyednleq vsggslstai
      421 lnrsnpkypq qtastvastt attslnrese fnemaawldh tpgahgdqes ltsseferfl
      481 aeraaaaeal ptlpattatt gttnsensni qrqinkdqdk slfal
//
LOCUS       XP_034170806             508 aa            linear   INV 03-SEP-2025
DEFINITION  TOM1-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034170806
VERSION     XP_034170806.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034314915.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="TOM1-like protein 2 isoform X2"
                     /calculated_mol_wt=56159
     Region          14..151
                     /region_name="VHS_Tom1_like"
                     /note="VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily;
                     cd03565"
                     /db_xref="CDD:340768"
     Site            order(32..33,36,40,83..84)
                     /site_type="other"
                     /note="putative intraprotein/interprotein binding site
                     [polypeptide binding]"
                     /db_xref="CDD:340768"
     Site            order(50,54,57..58)
                     /site_type="other"
                     /note="putative membrane binding residues"
                     /db_xref="CDD:340768"
     Region          216..308
                     /region_name="GAT_TOM1_like"
                     /note="canonical GAT domain found in target of myb protein
                     1 (Tom1) protein family; cd14233"
                     /db_xref="CDD:410581"
     Site            order(224..225,228..229,231..232,235..236,245,258,
                     261..262)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:410581"
     CDS             1..508
                     /gene="LOC117612087"
                     /coded_by="XM_034314915.2:245..1771"
                     /db_xref="GeneID:117612087"
ORIGIN      
        1 msffgvnvnp fstpvgqkie qatdgslpse nwtlnmeicd iinetedgpr daikaikrrl
       61 nqaagknyti vmytltvlet cvkncgkrfh tlacsrefiq elvkligpkn epptavqekv
      121 lsliqtwadt frhqphtqgv vqvyqelkvk giqfpmtdld amapiitper svpeseqsii
      181 nvptteqqsi tpatlqvqqp pnqssgqliq lneqqmaklq seldvvqgnm rvlsemlaff
      241 tssdqnnsqq pdpadfellt elhstckamq ervvdligkl ahdemtaell rindelnnlf
      301 lrysrytknk avaastilaq tighpqnmds assnkkeepd slidlsdetd tlekkvtgmd
      361 iadnadknri erkekkegdn defdmfaqsr nttyettkns gskyednleq vsggslstai
      421 lnrsnpkypq qtastvastt attspgahgd qesltssefe rflaeraaaa ealptlpatt
      481 attgttnsen sniqrqinkd qdkslfal
//
LOCUS       XP_034170814             491 aa            linear   INV 03-SEP-2025
DEFINITION  TOM1-like protein 2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034170814
VERSION     XP_034170814.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034314923.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..491
                     /product="TOM1-like protein 2 isoform X3"
                     /calculated_mol_wt=54824
     Region          14..151
                     /region_name="VHS_Tom1_like"
                     /note="VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily;
                     cd03565"
                     /db_xref="CDD:340768"
     Site            order(32..33,36,40,83..84)
                     /site_type="other"
                     /note="putative intraprotein/interprotein binding site
                     [polypeptide binding]"
                     /db_xref="CDD:340768"
     Site            order(50,54,57..58)
                     /site_type="other"
                     /note="putative membrane binding residues"
                     /db_xref="CDD:340768"
     Region          216..308
                     /region_name="GAT_TOM1_like"
                     /note="canonical GAT domain found in target of myb protein
                     1 (Tom1) protein family; cd14233"
                     /db_xref="CDD:410581"
     Site            order(224..225,228..229,231..232,235..236,245,258,
                     261..262)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:410581"
     CDS             1..491
                     /gene="LOC117612087"
                     /coded_by="XM_034314923.2:252..1727"
                     /db_xref="GeneID:117612087"
ORIGIN      
        1 msffgvnvnp fstpvgqkie qatdgslpse nwtlnmeicd iinetedgpr daikaikrrl
       61 nqaagknyti vmytltvlet cvkncgkrfh tlacsrefiq elvkligpkn epptavqekv
      121 lsliqtwadt frhqphtqgv vqvyqelkvk giqfpmtdld amapiitper svpeseqsii
      181 nvptteqqsi tpatlqvqqp pnqssgqliq lneqqmaklq seldvvqgnm rvlsemlaff
      241 tssdqnnsqq pdpadfellt elhstckamq ervvdligkl ahdemtaell rindelnnlf
      301 lrysrytknk avaastilaq tighpqnmds assnkkeepd slidlsdetd tlekkvtgmd
      361 iadnadknri erkekkegdn defdmfaqsr nttyettkns gskyednleq lnresefnem
      421 aawldhtpga hgdqesltss eferflaera aaaealptlp attattgttn sensniqrqi
      481 nkdqdkslfa l
//
LOCUS       XP_034170824             474 aa            linear   INV 03-SEP-2025
DEFINITION  TOM1-like protein 2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034170824
VERSION     XP_034170824.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034314933.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="TOM1-like protein 2 isoform X4"
                     /calculated_mol_wt=52779
     Region          14..151
                     /region_name="VHS_Tom1_like"
                     /note="VHS (Vps27/Hrs/STAM) domain of Tom1 subfamily;
                     cd03565"
                     /db_xref="CDD:340768"
     Site            order(32..33,36,40,83..84)
                     /site_type="other"
                     /note="putative intraprotein/interprotein binding site
                     [polypeptide binding]"
                     /db_xref="CDD:340768"
     Site            order(50,54,57..58)
                     /site_type="other"
                     /note="putative membrane binding residues"
                     /db_xref="CDD:340768"
     Region          216..308
                     /region_name="GAT_TOM1_like"
                     /note="canonical GAT domain found in target of myb protein
                     1 (Tom1) protein family; cd14233"
                     /db_xref="CDD:410581"
     Site            order(224..225,228..229,231..232,235..236,245,258,
                     261..262)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:410581"
     CDS             1..474
                     /gene="LOC117612087"
                     /coded_by="XM_034314933.2:250..1674"
                     /db_xref="GeneID:117612087"
ORIGIN      
        1 msffgvnvnp fstpvgqkie qatdgslpse nwtlnmeicd iinetedgpr daikaikrrl
       61 nqaagknyti vmytltvlet cvkncgkrfh tlacsrefiq elvkligpkn epptavqekv
      121 lsliqtwadt frhqphtqgv vqvyqelkvk giqfpmtdld amapiitper svpeseqsii
      181 nvptteqqsi tpatlqvqqp pnqssgqliq lneqqmaklq seldvvqgnm rvlsemlaff
      241 tssdqnnsqq pdpadfellt elhstckamq ervvdligkl ahdemtaell rindelnnlf
      301 lrysrytknk avaastilaq tighpqnmds assnkkeepd slidlsdetd tlekkvtgmd
      361 iadnadknri erkekkegdn defdmfaqsr nttyettkns gskyednleq pgahgdqesl
      421 tsseferfla eraaaaealp tlpattattg ttnsensniq rqinkdqdks lfal
//
LOCUS       XP_034178925             445 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178925
VERSION     XP_034178925.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="DNA polymerase delta subunit 2 isoform X1"
                     /calculated_mol_wt=49956
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..442
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..445
                     /gene="LOC117603651"
                     /coded_by="XM_034323034.2:95..1432"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftaeeg qmvrlicipk
      421 fsetqtvvlv dlenlkswpv sfain
//
LOCUS       XP_034179011             445 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179011
VERSION     XP_034179011.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323120.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="DNA polymerase delta subunit 2 isoform X1"
                     /calculated_mol_wt=49956
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..442
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..445
                     /gene="LOC117603651"
                     /coded_by="XM_034323120.2:95..1432"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftaeeg qmvrlicipk
      421 fsetqtvvlv dlenlkswpv sfain
//
LOCUS       XP_034179093             445 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179093
VERSION     XP_034179093.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323202.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="DNA polymerase delta subunit 2 isoform X1"
                     /calculated_mol_wt=49956
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..442
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..445
                     /gene="LOC117603651"
                     /coded_by="XM_034323202.2:95..1432"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftaeeg qmvrlicipk
      421 fsetqtvvlv dlenlkswpv sfain
//
LOCUS       XP_034179173             444 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179173
VERSION     XP_034179173.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323282.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..444
                     /product="DNA polymerase delta subunit 2 isoform X2"
                     /calculated_mol_wt=49885
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..441
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..444
                     /gene="LOC117603651"
                     /coded_by="XM_034323282.2:130..1464"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklfteegq mvrlicipkf
      421 setqtvvlvd lenlkswpvs fain
//
LOCUS       XP_034179252             422 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179252
VERSION     XP_034179252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323361.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="DNA polymerase delta subunit 2 isoform X3"
                     /calculated_mol_wt=47484
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..407
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..422
                     /gene="LOC117603651"
                     /coded_by="XM_034323361.2:95..1363"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftdffg ifdsdqlliy
      421 iq
//
LOCUS       XP_034179340             422 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179340
VERSION     XP_034179340.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323449.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="DNA polymerase delta subunit 2 isoform X3"
                     /calculated_mol_wt=47484
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..407
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..422
                     /gene="LOC117603651"
                     /coded_by="XM_034323449.2:95..1363"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftdffg ifdsdqlliy
      421 iq
//
LOCUS       XP_076545977             422 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545977
VERSION     XP_076545977.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689862.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="DNA polymerase delta subunit 2 isoform X3"
                     /calculated_mol_wt=47484
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..407
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..422
                     /gene="LOC117603651"
                     /coded_by="XM_076689862.1:95..1363"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftdffg ifdsdqlliy
      421 iq
//
LOCUS       XP_034179423             410 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179423
VERSION     XP_034179423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="DNA polymerase delta subunit 2 isoform X4"
                     /calculated_mol_wt=46019
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..406
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..410
                     /gene="LOC117603651"
                     /coded_by="XM_034323532.2:95..1327"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftvtsy
//
LOCUS       XP_034179506             409 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179506
VERSION     XP_034179506.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323615.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="DNA polymerase delta subunit 2 isoform X5"
                     /calculated_mol_wt=45944
     Region          32..162
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          183..406
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(189,191,232,286..287,333,396)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(189,191,232,286,333,396)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..409
                     /gene="LOC117603651"
                     /coded_by="XM_034323615.2:95..1324"
                     /db_xref="GeneID:117603651"
ORIGIN      
        1 mevdpplvyr rksfefqdfg kfkivsrdfk kqycntyiar laalrkhliq kaklkwaqhe
       61 ivtlaelser neskncilig tlykhqelkp silrelgkel qlvtqpartn yasskdilyl
      121 edevlrikld gnhvnvkdvv tgvicavlgr elengafsvi dwclpgccpk pstcnqlssr
      181 kgkilimsgl dlvnntesls lsllsewitg migckrnqee vasivciiia gniirgsvei
      241 hnhkgyfenk theesvfkkt amathkldnf lcpivqncpv ilmpgefdpt chtipqqplh
      301 pcilpqcarf ksfygatnpw igsinsciva gssgqpitdi mkvadianvs pltwlkrtld
      361 wqhyaptgpd tiptypcdev dpfvmtecpd vyfagnmdky dtklftviy
//
LOCUS       XP_034179615             411 aa            linear   INV 03-SEP-2025
DEFINITION  diphthamide biosynthesis 1 [Osmia lignaria lignaria].
ACCESSION   XP_034179615
VERSION     XP_034179615.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323724.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="diphthamide biosynthesis 1"
                     /calculated_mol_wt=45958
     Region          73..371
                     /region_name="Diphthamide_syn"
                     /note="Putative diphthamide synthesis protein; pfam01866"
                     /db_xref="CDD:460365"
     CDS             1..411
                     /gene="Dph1"
                     /coded_by="XM_034323724.2:302..1537"
                     /db_xref="GeneID:117604026"
ORIGIN      
        1 msegcdsvvv vkakpvrkvf kapfkinkip eeilndqlln aaiaalpsny nfeihksiwr
       61 ikeakvkrva lqmpegllmy attiadiiee fteaetvimg dvtygaccid dytakaldad
      121 flihyghscl ipidqtvgik vlyvfvniki dtlhcieclq atlpittkia lvstiqfvnt
      181 lqavalemrk ngyeisipqs kplspgeilg ctapqircvd aviyvgdgrf hleaamianp
      241 klqafrydpy ekkmtqefyn heemlktrlt atehakessr fglilgtlgr qgninvlrni
      301 enkinslgke yviillseif pdkiklfkgi dafiqvacpr lsidwgtafe kpfltpyega
      361 valkmvnhng ekpypmdfya sislgpwtpn ykecdlekqi dtccgkckdk t
//
LOCUS       XP_034179831             152 aa            linear   INV 03-SEP-2025
DEFINITION  class E basic helix-loop-helix protein 23 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179831
VERSION     XP_034179831.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323940.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179831.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..152
                     /product="class E basic helix-loop-helix protein 23"
                     /calculated_mol_wt=17571
     Region          55..114
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(63..64,66,71,91..92)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381555"
     Site            order(69..70,73..74,76..77,81,92..93,96..97,100,102..103,
                     106..107)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381555"
     CDS             1..152
                     /gene="LOC117604092"
                     /coded_by="XM_034323940.2:424..882"
                     /db_xref="GeneID:117604092"
ORIGIN      
        1 mtskimngdk wkgkwpkqqs qanktvrrni rrneksglkl akssrgttpr ertlrrlesn
       61 erermrmhsl ndafqslrev iphvtkerrl skietltlak nyivaltdvi cemrneekat
      121 dqrievsesq essknmnicl nmnipipsks cs
//
LOCUS       XP_034179957             149 aa            linear   INV 03-SEP-2025
DEFINITION  MAPK regulated corepressor interacting protein 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179957
VERSION     XP_034179957.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324066.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="MAPK regulated corepressor interacting protein
                     2"
                     /calculated_mol_wt=17148
     Region          47..146
                     /region_name="FAM195"
                     /note="FAM195 family; pfam14799"
                     /db_xref="CDD:464321"
     CDS             1..149
                     /gene="LOC117604185"
                     /coded_by="XM_034324066.2:184..633"
                     /db_xref="GeneID:117604185"
ORIGIN      
        1 mytvskgpsk ivaktrrgis qnlerletlr dltrkadpdd dhestrhvpk pvfhmngksk
       61 lssqrhqiqe vitpqheeli kfvyeswnqv ntrqrsessd gsdcsepssp nsivyyndge
      121 pndtlqdfkp fdleswwgkr lfnnitksl
//
LOCUS       XP_034180070            1021 aa            linear   INV 03-SEP-2025
DEFINITION  folliculin [Osmia lignaria lignaria].
ACCESSION   XP_034180070
VERSION     XP_034180070.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324179.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180070.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1021
                     /product="folliculin"
                     /calculated_mol_wt=114301
     Region          1..567
                     /region_name="PRK14479"
                     /note="dihydroxyacetone kinase; Provisional; cl29418"
                     /db_xref="CDD:475197"
     Region          602..761
                     /region_name="Folliculin"
                     /note="Vesicle coat protein involved in Golgi to plasma
                     membrane transport; pfam11704"
                     /db_xref="CDD:463323"
     Region          828..1018
                     /region_name="Folliculin_C"
                     /note="Folliculin C-terminal domain; pfam16692"
                     /db_xref="CDD:465236"
     CDS             1..1021
                     /gene="BHD"
                     /coded_by="XM_034324179.2:143..3208"
                     /db_xref="GeneID:117604261"
ORIGIN      
        1 mstlihdytn eiigksllgm tklhnglivl encntvlhkn ytnmtgkvkl isggkvenel
       61 tytgfvgpgm ltaailgntf cappandilk vieevgfdhs sgilliiqnd teyiinftaa
      121 klqaeakgyi vkiitiksts hycgsttqeq dfspiifiyk iagamseegn nineiysvcn
      181 sianngemtl lkaeyerkmc keyelemfsn lakqilepvi nilnsgsegq snicemktfh
      241 tgheiaiiin nfghnqietn tfilelvkqv etyglktkri yvkslmtdvs gfhicllnls
      301 ftkvlikyld fptyapawpk vltfetmnik nddttqlits ekyckgints diniqgpiis
      361 yetaqlflsv ismaceaiia ctnqlnkmdq qfgngdcgts larganaiqh aiqenkilgt
      421 nvcvtftqis qiiertvggv qgglyslffh niaktfseye ndkkltadmw lyalgkakei
      481 ieqleitsin dgtllhvlte vendlenaln rnmnpldafa ivvrtaenfa intvhtqssh
      541 ikrykelkyp npqahavgiw mraayevtnq nivedgqyec egcqsignvk ylsnehetrt
      601 sflsaqqslt qdiwcllkha cfrslscevh pgkegvcyfg deyrghvlsh tftlkdaqar
      661 gfrkwcsfiv fmrdkqflln mwpflvdnlk evirelqdfa ekkynaeeak cpqrivrlsa
      721 nngsgsynns nkqprtlisi tnekhvfiri hmwlvwilsa garhfveifp msllddelny
      781 nlehqieted gftlvnaklp vnlnlnsneq evtseflevs gksttvilrd lrivlgkeqf
      841 rqllyssltg vqilvrgpni qrleslygls slipracrrv ktqateymdp nkcnfigvdt
      901 svavpvpcan vcrldivsse hkienssshv vrwtgtlplk lptlltkiek sldneklgns
      961 tlkahfaalq eewaniakvv hamrgrghsg dlsglmlslg agphdkklld awsmglppnp
     1021 a
//
LOCUS       XP_034175124            1907 aa            linear   INV 03-SEP-2025
DEFINITION  histone acetyltransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034175124
VERSION     XP_034175124.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319233.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175124.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1907
                     /product="histone acetyltransferase 1"
                     /calculated_mol_wt=210623
     Region          356..381
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(373..374,377,380..381,419..420,423,426..427,430,
                     460..461,464,467..468,471,500..501,504,507..508,548..549,
                     552)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          402..430
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          442..472
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          484..508
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          528..554
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          606..1096
                     /region_name="DUF3535"
                     /note="Domain of unknown function (DUF3535); pfam12054"
                     /db_xref="CDD:463447"
     Region          <1268..1828
                     /region_name="HepA"
                     /note="Superfamily II DNA or RNA helicase, SNF2 family
                     [Transcription, Replication, recombination, and repair];
                     COG0553"
                     /db_xref="CDD:440319"
     CDS             1..1907
                     /gene="Hel89B"
                     /coded_by="XM_034319233.2:237..5960"
                     /db_xref="GeneID:117601876"
ORIGIN      
        1 mtsrldrlfi lletgtnavt kraaaqqlge aqrlhphdlh hllarvstll kspqwdtrvs
       61 aaqavqaila qvpawdpqsi kketctsdge akrpsnklnl edfnmekvla rsshltgseg
      121 seydliatdg eqmslsnqee kliaaklglh prlmgvdtse lftaedltpv vlpptsnaqn
      181 kvsvnetlkq pgglsrremn rarrkarqsv skqrsrepdd hrsnedhhnn snaqspintg
      241 epfnkkikle evisseigvt csaqnestns vpdstgcwpd svidwplesf aenlcqdlfs
      301 qkweirhgaa talrelvklh gkgagksrdq tadqmeenhh qwiidaalrl lcvlgldrfg
      361 dfvsdqvvap vretcaqalg sllllvpnqk ddknenkgii gilsvmlkll ehdewearhg
      421 allalkylla vrddllddil prvfpaimqg lsdpvddvga aaasalipva salprllkps
      481 eleaivirlw ellreqddla aacnsfmgll aailslptar tcitpqplsq vlprlwpfls
      541 hssssvrkat lqtlqtltge dgahnenkre rwgdggglvl qealrhvfqr vliehvtaiq
      601 dvaervwdnl vvqsdlelll haacplvstw lclamqpehv pfnpnllmti stlnkgiknn
      661 qiidicdgqs dtsnnggnnt vtgnvksvse lkvyiggiet vaqsirksnv vqarcrasrm
      721 lgllshyvvq papgvtytpd ipspaicyak vllahlnsrs alqrtvaglt mshwatindm
      781 kppaipdilr drllgclnec vyydeiaasf trllhdsrdy iatlkhyklp vpietdasgv
      841 mtldqiasla gkpiselcai snatgsggpa gnisgntstv klkpkliesl eerrkalevg
      901 aadtaaqqls ynvmsmaala gaatmlhclp ptpqplnplv kplmeaikre eneelqklaa
      961 khlsylvdlc vnrkpspnak istnlctflc sdieftprvn cntdsdlfdg iltlsnrqkh
     1021 aervaynrga nsglgggrgp grpptteipl eellaceepe akaartrrrg atfaltaiat
     1081 lfgvqlptrl phlwelilpn ilreedkitg gknsqeevnq lifalqvlei mapsldkall
     1141 ppaleclscl ckllahpyka vrhmasrcia vlatldtekv vvhitrsvip lleatggekv
     1201 ysmtvtapse vdsirqgaae altclvenlg vnivpyavlf mvpllgrmsd qnqavrlacs
     1261 atfatlvqll pldpgaianl pdlvekkaqe rrfleqllnp rsipdtelpi pvaaelrsyq
     1321 rqglnwlnfl nryrlhgvlc ddmglgktlq tlcilaldhh rnphappslv vcpptltghw
     1381 vyeadkffkt kdlsvlqyag tppereklrp kvtyhrlvva sydivrkdie yfetrqwnyc
     1441 vldeghvikn gktksakatk rlhanhrlil sgtpvqndvl elwslfdflm pgflgtekqf
     1501 aakysrpila crepkagpke qeggalamea lhrqvlpfll rrnkedvlqd lppkitqdyy
     1561 cdlsplqrtl yedfrtrhsa tllsstscts sasdphgghv fealrylrnv cnhpklvlnp
     1621 rhplyqnicn tlkqqkstla didhgaklpa lkqllldcgi gqpqqqqsrc nsvatgatqd
     1681 nqppqqqqlv sqhralifcq lkamldivek dllrthlptv tylrldgsip atqrhsvvar
     1741 fnadpsidvl llttqvgglg lnltgadtvi fvehdwnpmk dlqamdrahr igqkkvvnvy
     1801 rlitrstvee kimglqkfkl ltantviste nasletmatd qlldlfslen nkgkrsdtqe
     1861 davskisnvp gvsrsvleil pelweqqqyd deydfdsfls tlksdnq
//
LOCUS       XP_034175206            1907 aa            linear   INV 03-SEP-2025
DEFINITION  histone acetyltransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034175206
VERSION     XP_034175206.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319315.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175206.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1907
                     /product="histone acetyltransferase 1"
                     /calculated_mol_wt=210623
     Region          356..381
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(373..374,377,380..381,419..420,423,426..427,430,
                     460..461,464,467..468,471,500..501,504,507..508,548..549,
                     552)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          402..430
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          442..472
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          484..508
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          528..554
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          606..1096
                     /region_name="DUF3535"
                     /note="Domain of unknown function (DUF3535); pfam12054"
                     /db_xref="CDD:463447"
     Region          <1268..1828
                     /region_name="HepA"
                     /note="Superfamily II DNA or RNA helicase, SNF2 family
                     [Transcription, Replication, recombination, and repair];
                     COG0553"
                     /db_xref="CDD:440319"
     CDS             1..1907
                     /gene="Hel89B"
                     /coded_by="XM_034319315.2:219..5942"
                     /db_xref="GeneID:117601876"
ORIGIN      
        1 mtsrldrlfi lletgtnavt kraaaqqlge aqrlhphdlh hllarvstll kspqwdtrvs
       61 aaqavqaila qvpawdpqsi kketctsdge akrpsnklnl edfnmekvla rsshltgseg
      121 seydliatdg eqmslsnqee kliaaklglh prlmgvdtse lftaedltpv vlpptsnaqn
      181 kvsvnetlkq pgglsrremn rarrkarqsv skqrsrepdd hrsnedhhnn snaqspintg
      241 epfnkkikle evisseigvt csaqnestns vpdstgcwpd svidwplesf aenlcqdlfs
      301 qkweirhgaa talrelvklh gkgagksrdq tadqmeenhh qwiidaalrl lcvlgldrfg
      361 dfvsdqvvap vretcaqalg sllllvpnqk ddknenkgii gilsvmlkll ehdewearhg
      421 allalkylla vrddllddil prvfpaimqg lsdpvddvga aaasalipva salprllkps
      481 eleaivirlw ellreqddla aacnsfmgll aailslptar tcitpqplsq vlprlwpfls
      541 hssssvrkat lqtlqtltge dgahnenkre rwgdggglvl qealrhvfqr vliehvtaiq
      601 dvaervwdnl vvqsdlelll haacplvstw lclamqpehv pfnpnllmti stlnkgiknn
      661 qiidicdgqs dtsnnggnnt vtgnvksvse lkvyiggiet vaqsirksnv vqarcrasrm
      721 lgllshyvvq papgvtytpd ipspaicyak vllahlnsrs alqrtvaglt mshwatindm
      781 kppaipdilr drllgclnec vyydeiaasf trllhdsrdy iatlkhyklp vpietdasgv
      841 mtldqiasla gkpiselcai snatgsggpa gnisgntstv klkpkliesl eerrkalevg
      901 aadtaaqqls ynvmsmaala gaatmlhclp ptpqplnplv kplmeaikre eneelqklaa
      961 khlsylvdlc vnrkpspnak istnlctflc sdieftprvn cntdsdlfdg iltlsnrqkh
     1021 aervaynrga nsglgggrgp grpptteipl eellaceepe akaartrrrg atfaltaiat
     1081 lfgvqlptrl phlwelilpn ilreedkitg gknsqeevnq lifalqvlei mapsldkall
     1141 ppaleclscl ckllahpyka vrhmasrcia vlatldtekv vvhitrsvip lleatggekv
     1201 ysmtvtapse vdsirqgaae altclvenlg vnivpyavlf mvpllgrmsd qnqavrlacs
     1261 atfatlvqll pldpgaianl pdlvekkaqe rrfleqllnp rsipdtelpi pvaaelrsyq
     1321 rqglnwlnfl nryrlhgvlc ddmglgktlq tlcilaldhh rnphappslv vcpptltghw
     1381 vyeadkffkt kdlsvlqyag tppereklrp kvtyhrlvva sydivrkdie yfetrqwnyc
     1441 vldeghvikn gktksakatk rlhanhrlil sgtpvqndvl elwslfdflm pgflgtekqf
     1501 aakysrpila crepkagpke qeggalamea lhrqvlpfll rrnkedvlqd lppkitqdyy
     1561 cdlsplqrtl yedfrtrhsa tllsstscts sasdphgghv fealrylrnv cnhpklvlnp
     1621 rhplyqnicn tlkqqkstla didhgaklpa lkqllldcgi gqpqqqqsrc nsvatgatqd
     1681 nqppqqqqlv sqhralifcq lkamldivek dllrthlptv tylrldgsip atqrhsvvar
     1741 fnadpsidvl llttqvgglg lnltgadtvi fvehdwnpmk dlqamdrahr igqkkvvnvy
     1801 rlitrstvee kimglqkfkl ltantviste nasletmatd qlldlfslen nkgkrsdtqe
     1861 davskisnvp gvsrsvleil pelweqqqyd deydfdsfls tlksdnq
//
LOCUS       XP_034170834             634 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear pore complex protein Nup75 [Osmia lignaria lignaria].
ACCESSION   XP_034170834
VERSION     XP_034170834.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034314943.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034170834.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..634
                     /product="nuclear pore complex protein Nup75"
                     /calculated_mol_wt=71720
     Region          50..598
                     /region_name="Nucleopor_Nup85"
                     /note="Nup85 Nucleoporin; pfam07575"
                     /db_xref="CDD:400113"
     CDS             1..634
                     /gene="Nup75"
                     /coded_by="XM_034314943.2:231..2135"
                     /db_xref="GeneID:117600021"
ORIGIN      
        1 maetdnppiv gtlsisknvg iagswinsnk isiygskrln kechekydpt vhilrpevil
       61 fspqlrklvn esngvflsiq kielgsgdvr pellkhskqy rsilracies lqdicgksls
      121 dkkeslenfl tifyqiecvw hlteilyidv vpgdivlpql lewirfhfpa relialkila
      181 qktigadleh pnywetvigc alhgkidlvr allalhskad hpafvmaeni lktmplynvy
      241 ggysinefmm rwkhwqldls sninnkafvs dtnlemimkl ivgeqdvlwq fssytdawye
      301 llaaklfyts pcckqpelsl hanniskrwq ankpsdnaih aliesnlhqv ikeiqhmgdn
      361 gwfaahlvnl lytcgklkil dkdqievtsq lheslileyg ntlmghhslw qcgasylihc
      421 psqglarlei llqslpmgse arvnkiidia rdnkmdhivt smckiqgiks iqqgrlgnal
      481 awalkaqdss fttyiadqfl khyaesgkle crdllenlgf cmmasdrltf lgkycefhqm
      541 ygigefkeaa sllvsllvsn ltpkyfwsil lsdaipllea kdvilsssdc fellrcaeah
      601 gddpkfqnei eifrlavarn laralslegc qveh
//
LOCUS       XP_034175325             610 aa            linear   INV 03-SEP-2025
DEFINITION  alkyldihydroxyacetonephosphate synthase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175325
VERSION     XP_034175325.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175325.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="alkyldihydroxyacetonephosphate synthase isoform
                     X1"
                     /calculated_mol_wt=68346
     Region          141..604
                     /region_name="GlcD"
                     /note="FAD/FMN-containing lactate dehydrogenase/glycolate
                     oxidase [Energy production and conversion]; COG0277"
                     /db_xref="CDD:440046"
     CDS             1..610
                     /gene="Agps"
                     /coded_by="XM_034319434.2:225..2057"
                     /db_xref="GeneID:117601992"
ORIGIN      
        1 mssnpkngan envenlngpi cvqsvvpker qdllkwngwg ykdsefrine kniieftgdr
       61 ypignkelpy ftqwvkdmfg vdlnkkqpsq pmptnlpepi islelleaiq elkieystkg
      121 idrlvrahgh tlrdiyllrh gsfkrvpdiv vwpkchedvv kiiklcahyg svcipfgggt
      181 svsgaascpe nerrtiilld tsqmnrilwl drenliicce tgiigqdler qlrlqgltsg
      241 hepdsyefss lggwvatras gmkknrygni edlvvrvrmv tgriddpeit lergtlvpra
      301 scgpdfdhvi lgsegtlgvi tevvlkirpi pkivkygsiv fpnfqtgvsa lrqvakdrcq
      361 pasirlmdne qfhfgqalkp epgwgglivq glkqayitri krfkfdqmcv atllfegdta
      421 advaaqekkv yniakryngv pagesngerg yvltfviayi rdmglnyyvl aesfetsvaw
      481 nrtvflcrnv ksrvarecya rgvehyfisc ritqtydsgc cvyfylainy nklsnpveay
      541 esiehiarqe ilanggslsh hhgvgklrsc fypnavgevg vslyraakah ldphnifaag
      601 nldprcvskl
//
LOCUS       XP_034175417             581 aa            linear   INV 03-SEP-2025
DEFINITION  alkyldihydroxyacetonephosphate synthase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175417
VERSION     XP_034175417.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319526.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="alkyldihydroxyacetonephosphate synthase isoform
                     X2"
                     /calculated_mol_wt=65269
     Region          112..575
                     /region_name="GlcD"
                     /note="FAD/FMN-containing lactate dehydrogenase/glycolate
                     oxidase [Energy production and conversion]; COG0277"
                     /db_xref="CDD:440046"
     CDS             1..581
                     /gene="Agps"
                     /coded_by="XM_034319526.2:211..1956"
                     /db_xref="GeneID:117601992"
ORIGIN      
        1 mqdllkwngw gykdsefrin ekniieftgd rypignkelp yftqwvkdmf gvdlnkkqps
       61 qpmptnlpep iislelleai qelkieystk gidrlvrahg htlrdiyllr hgsfkrvpdi
      121 vvwpkchedv vkiiklcahy gsvcipfggg tsvsgaascp enerrtiill dtsqmnrilw
      181 ldrenliicc etgiigqdle rqlrlqglts ghepdsyefs slggwvatra sgmkknrygn
      241 iedlvvrvrm vtgriddpei tlergtlvpr ascgpdfdhv ilgsegtlgv itevvlkirp
      301 ipkivkygsi vfpnfqtgvs alrqvakdrc qpasirlmdn eqfhfgqalk pepgwggliv
      361 qglkqayitr ikrfkfdqmc vatllfegdt aadvaaqekk vyniakryng vpagesnger
      421 gyvltfviay irdmglnyyv laesfetsva wnrtvflcrn vksrvarecy argvehyfis
      481 critqtydsg ccvyfylain ynklsnpvea yesiehiarq eilanggsls hhhgvgklrs
      541 cfypnavgev gvslyraaka hldphnifaa gnldprcvsk l
//
LOCUS       XP_034170844             124 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase, subunit F [Osmia lignaria lignaria].
ACCESSION   XP_034170844
VERSION     XP_034170844.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034314953.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="ATP synthase, subunit F"
                     /calculated_mol_wt=14165
     Region          20..119
                     /region_name="WRW"
                     /note="Mitochondrial F1F0-ATP synthase, subunit f;
                     pfam10206"
                     /db_xref="CDD:431134"
     CDS             1..124
                     /gene="ATPsynF"
                     /coded_by="XM_034314953.2:117..491"
                     /db_xref="GeneID:117600030"
ORIGIN      
        1 matpptdpsv vnpitkwvri gdypvgydpa khgpynpsry ygpadtpfge lkikeipawi
       61 grrnltpqgv aglfsralwr wqmtwvlprk sspapifqff iagstlfyll nylrlrghrs
      121 ykyh
//
LOCUS       XP_034186212             635 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607063 [Osmia lignaria lignaria].
ACCESSION   XP_034186212
VERSION     XP_034186212.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330321.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186212.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..635
                     /product="uncharacterized protein LOC117607063"
                     /calculated_mol_wt=68160
     CDS             1..635
                     /gene="LOC117607063"
                     /coded_by="XM_034330321.2:74..1981"
                     /db_xref="GeneID:117607063"
ORIGIN      
        1 mkfllitcll atsllcvyga eetedkpeit klelvelgeg esdddaedsd vqrkrdsgys
       61 ykrpdnfala srsrfqvgqs ghrgrivnrh paqinrpitk ygppgyqnss pmrpvshgqq
      121 hrdkfqfhgh fgqqhsnnfd grpsglleqg vpspirqsdf aepnpistqn nepfathsan
      181 ylppqnqklp gfgtvqafss ahaaaqnips ndqgqsanfq sqnlvqsqgq isdaalfltq
      241 naqaiqqlyg qppneqdfap vngdqflghs nqvhngqfqn festsqspqn fpgqlpsyas
      301 gtlsaqetle qiqslekdrl ivqlqralas qaqnadasgr yaenhpsfvq sqdllaslgq
      361 rmkihglnaq pttvtfgsgn tafnqspflp gttispgfpl syglsttaqp pttttptttt
      421 slqppqatkg dgtsqpgssl pappsapavp vyggfvptli tgtsflsnvp sygptffapg
      481 avtpvqpsgs spthfglpip adhgqkptgt tptsvspttp stpstsrpgl pssppvntvp
      541 vplhpvvtpl hpvapllppv tpshlhpvha pstghptygl qtplinplly kpikpvypfy
      601 yyqnlayqph klalptypws yaptyaqakp aqiwk
//
LOCUS       XP_034179993             478 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604244 [Osmia lignaria lignaria].
ACCESSION   XP_034179993
VERSION     XP_034179993.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324102.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="uncharacterized protein LOC117604244"
                     /calculated_mol_wt=53595
     CDS             1..478
                     /gene="LOC117604244"
                     /coded_by="XM_034324102.2:95..1531"
                     /db_xref="GeneID:117604244"
ORIGIN      
        1 mellllflla ptilvqgdgk sndpndepfl piypvypynp klikrgtdre tatqlspely
       61 apkvdakdsy sasytanypr dpyypnynpy pkvssssgys yystipysyq ttpyntytsy
      121 sspysasppt yasipysssy pnlyyqhpyy ypghysqslf ppppppplpl pgtdytgdpy
      181 sdqgsteknr dkngdkryrd sdvnqepvgn qfvdggnyis gnpkdldsqp styktsspqn
      241 qlnqdpqikn lpiplpktty rvisvagqpv gpdyplpsty vkaqqleelv shtwakllaq
      301 nlqqqnaqna eqdatkvvan qndqgnnsqr llaylvnpsi lgklnvaqtv gqlvqapttr
      361 lknikypplt pgvytavekp ekdqteseye nyensssqga qdydasssqn dkqpqsyend
      421 sarsvsyqnq nfvtvetprr ynyqysnynp sqtitqqqsq qyknnlddsn fgaktkng
//
LOCUS       XP_034186334             510 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117607119 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186334
VERSION     XP_034186334.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330443.2
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186334.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117607119"
                     /calculated_mol_wt=49756
     CDS             1..510
                     /gene="LOC117607119"
                     /coded_by="XM_034330443.2:16..1548"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 1 codon"
                     /db_xref="GeneID:117607119"
ORIGIN      
        1 mpsettisln ilgsylttrn ieqedxlftr nmrsliilvl avvaistvss retrgemrvl
       61 rplakgkrgv sdysggyssg gssysspihs sgssgysgas lggyslghss piqslggglg
      121 hgsvgyslgs ggsslghgls lqglslgghy gqpsysgggg sialfspssk tgpvtfglhg
      181 gglssgssgs sgysspiyat gvhglssyss ggssggislh pamlgsshga tfslpaasss
      241 shsipiqsis qsssypvsgg llidsgslgk sssysqggss lgslhgssgg sgysipissg
      301 shgisslssn sggsvsyslp vssgshgiss lsshsggsgg yslpvssgss nshisyssgs
      361 sdassyslpa ssvsyssgss gsnyipsssd gssyasggss sysspsssys nsgssysspi
      421 ssysspsssy sspsssyssp svtysgssgg ysqsysspss sygtpaeshg sysnlspryv
      481 gyasgksyds snsasnkydt isysspngky
//
LOCUS       XP_034186469             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607178 [Osmia lignaria lignaria].
ACCESSION   XP_034186469
VERSION     XP_034186469.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330578.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117607178"
                     /calculated_mol_wt=34314
     Region          46..>257
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(50,52..53,55,74..76,156..158,205..206,208,223,227,
                     253..256)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(180,208,223,227)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..320
                     /gene="LOC117607178"
                     /coded_by="XM_034330578.2:245..1207"
                     /db_xref="GeneID:117607178"
ORIGIN      
        1 mdaetgaila lqilalcsiv aallaylmrq srnatdeyet rnkrhvlvts cdtcvglqia
       61 ialyevgykv fvgvldpsgn spsmkilrai eqqkekedep gdtgrgnpqd pevrargqiv
      121 pleldstred slracldavr aklpagedgl wavvhtggla lpgvierqps sawesmlrhn
      181 lvaplrtarm fipllrvkrg rivllgdstt sygtkagtgl vafsasrkav egaaealkse
      241 lqssgvdvvl lkpppvnpli lysspvlkts dvesgivsse gtwtapmsth aiqnslipal
      301 ttpcprtsyd mvdkpklfcr
//
LOCUS       XP_034194517             298 aa            linear   INV 03-SEP-2025
DEFINITION  zwei Ig domain protein zig-8 [Osmia lignaria lignaria].
ACCESSION   XP_034194517
VERSION     XP_034194517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338626.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="zwei Ig domain protein zig-8"
                     /calculated_mol_wt=32874
     Region          58..149
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          76..80
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          89..94
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          131..135
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          145..150
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          157..160
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          175..263
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          185..189
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          200..204
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          226..236
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          246..251
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..298
                     /gene="LOC117610745"
                     /coded_by="XM_034338626.2:101..997"
                     /db_xref="GeneID:117610745"
ORIGIN      
        1 malrwqarls iaailfmcte dtlslgdrti dmdrhvsqsr prnqllvdps svkakaeptf
       61 dysqntnvta ligktayltc rvrnvgdktv swvrnrdihi ltagsytyts dqrfqalhkk
      121 ntgqnsewse wtlcikwaqe rdqglyecqi stipvkshqf rlnvvvptat ilggpdlyvg
      181 agstinltcv ihfsseppay ifwyynkqvl sydsprggis vitekggdvt tswlliqtaq
      241 psdsgeysck psnantasik vhvlngerpe amqtgtagpi lssscllvsl iilyissv
//
LOCUS       XP_034186698             282 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA N(3)-cytidine methyltransferase METTL6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186698
VERSION     XP_034186698.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330807.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="tRNA N(3)-cytidine methyltransferase METTL6"
                     /calculated_mol_wt=33166
     Region          94..195
                     /region_name="Methyltransf_12"
                     /note="Methyltransferase domain; pfam08242"
                     /db_xref="CDD:400515"
     Site            order(96..102,121..122,146..148,167)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..282
                     /gene="LOC117607261"
                     /coded_by="XM_034330807.2:174..1022"
                     /db_xref="GeneID:117607261"
ORIGIN      
        1 msyekttesi dgnmeyvghv akrltseeie kmqaqnsrlv sefqanqlek dakkhwdlfy
       61 krndtrffkd rhwttrefne llglsskedr nvllevgcgv gnfvyplved glkfrkifac
      121 dlspravelm knhmlyhpek mnifqtditm encfynvdcp vnvatlifvl saihpdkfrk
      181 vvknlynilh nggvvlfrdy glydmaqlrf kpghkisdnf ymrqdgtrty yfsmeeiskl
      241 fehagfktlt cdyiqrrtvn lkekidvpri fvqgkfekqi qv
//
LOCUS       XP_076545979             145 aa            linear   INV 03-SEP-2025
DEFINITION  adult-specific cuticular protein ACP-22-like [Osmia lignaria
            lignaria].
ACCESSION   XP_076545979
VERSION     XP_076545979.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689864.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..145
                     /product="adult-specific cuticular protein ACP-22-like"
                     /calculated_mol_wt=15692
     Region          65..115
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..145
                     /gene="LOC117604222"
                     /coded_by="XM_076689864.1:313..750"
                     /db_xref="GeneID:117604222"
ORIGIN      
        1 mapmeystrf vliatvlmma svqdgsagha hsfahfhgpv egpaeeitfh gkhgyhhgyv
       61 ahpkydfsyg vqdhhtgdhh sqkehrdgkn vageytvkep ggnvrtvkyh adphggffay
      121 vhnsngndhd gyggghghdg gsgyy
//
LOCUS       XP_034186827              90 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607322 [Osmia lignaria lignaria].
ACCESSION   XP_034186827
VERSION     XP_034186827.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330936.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..90
                     /product="uncharacterized protein LOC117607322"
                     /calculated_mol_wt=9752
     CDS             1..90
                     /gene="LOC117607322"
                     /coded_by="XM_034330936.2:142..414"
                     /db_xref="GeneID:117607322"
ORIGIN      
        1 mkcyiaiall alfavafaae qpaesekiep vvsaepqdap rdkrglllgy tapvaytsya
       61 apvaysasys vpyayrtyps ypyyssyylg
//
LOCUS       XP_034191202             451 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609238 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191202
VERSION     XP_034191202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..451
                     /product="uncharacterized protein LOC117609238 isoform X1"
                     /calculated_mol_wt=48418
     CDS             1..451
                     /gene="LOC117609238"
                     /coded_by="XM_034335311.2:2190..3545"
                     /db_xref="GeneID:117609238"
ORIGIN      
        1 mqagnhsmed mvtvassntp stntvhpsvn dmdwfagvae eellyytgvg ndvdslwavi
       61 gsfvpppprp pylpeeavtt dglttcdlcs wawrndrhsf sldgtleapg elgwvltlvi
      121 vslvsagiga vvmvtllhcr rlkstggras ccsvgvedsn vqeagatagg ggsgggvavi
      181 pdrppleldk lppyhdvaps pghnvwswlg srrgggtpgv vttplslpqh rramnlpven
      241 hythmqtdea lyaeldsqaa syasdlqlqm rgsstygtts ggqddeyhel daarlhrhyg
      301 gsngdgslrg dtlrtrhskr lqepdyemye ngpstpvppg qlqhhhhhhh hhnhhhhhqd
      361 lriggsrnge hpsyqntayt gsdaepdgpt lssapssayy sdlssnstnq qiptatssts
      421 ntnlhlnpqg lessqyrlaa inessvpsdy i
//
LOCUS       XP_034191211             415 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609238 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191211
VERSION     XP_034191211.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335320.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..415
                     /product="uncharacterized protein LOC117609238 isoform X2"
                     /calculated_mol_wt=44543
     CDS             1..415
                     /gene="LOC117609238"
                     /coded_by="XM_034335320.2:2191..3438"
                     /db_xref="GeneID:117609238"
ORIGIN      
        1 mqagnhsmed mvtvassntp stntvhpsvn dmdwfagvae eellyytgvg ndvdslwavi
       61 gsfvpppprp pylpeeavtt dglttcdlcs wawrndrhsf sldgtleapg elgwvltlvi
      121 vslvsagiga vvmvtllhcr rlkstggras ccsvgvedsn vqeagatagg ggsgggvavi
      181 pdrppleldk lppyhdvaps pvenhythmq tdealyaeld sqaasyasdl qlqmrgssty
      241 gttsggqdde yheldaarlh rhyggsngdg slrgdtlrtr hskrlqepdy emyengpstp
      301 vppgqlqhhh hhhhhhnhhh hhqdlriggs rngehpsyqn taytgsdaep dgptlssaps
      361 sayysdlssn stnqqiptat sstsntnlhl npqglessqy rlaainessv psdyi
//
LOCUS       XP_034191224             372 aa            linear   INV 03-SEP-2025
DEFINITION  L-lactate dehydrogenase [Osmia lignaria lignaria].
ACCESSION   XP_034191224
VERSION     XP_034191224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335333.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..372
                     /product="L-lactate dehydrogenase"
                     /calculated_mol_wt=40204
     Region          50..353
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(61..63,84..85,127..128,145,165..167,190,194,222,281)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:133419"
     Site            order(65,69,89,93,95..96,98..100,197..198,200..201,207,
                     283,287)
                     /site_type="other"
                     /note="LDH/MDH dimer interface [polypeptide binding]"
                     /db_xref="CDD:133419"
     Site            order(167,198,222)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:133419"
     CDS             1..372
                     /gene="LOC117609246"
                     /coded_by="XM_034335333.2:331..1449"
                     /db_xref="GeneID:117609246"
ORIGIN      
        1 meeeevqtap kndanepadr dscddedapf ptttknalit tqpgpcdayp hrvkvsivgv
       61 gkigiacaia ilmkrlasev wlidsdanka saeaediqhv gvflgsplvt gtsdfimvke
      121 savviittge tepgeepnvk rnlkafksfi pgvakfacks vlliatqptd vmsyiawkls
      181 gfpsnrvvgt gtmidcarfq dlvsrrlnva rssvscmtvg ahgnmavpiw ssihvggmkl
      241 rdinqrmgep ddpekwyeia envknmgkel eqkkgsccwg valstveivd aivrntkvvl
      301 pasthilsca hgtdkdvyms vpcvlgregv yctvrqklte qeksavqtca dkirsalrec
      361 gilqesaedv de
//
LOCUS       XP_034191237             345 aa            linear   INV 03-SEP-2025
DEFINITION  L-lactate dehydrogenase A-like 6A [Osmia lignaria lignaria].
ACCESSION   XP_034191237
VERSION     XP_034191237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335346.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..345
                     /product="L-lactate dehydrogenase A-like 6A"
                     /calculated_mol_wt=37411
     Region          30..339
                     /region_name="LDH_1"
                     /note="A subgroup of L-lactate dehydrogenases; cd05293"
                     /db_xref="CDD:133429"
     Site            order(30,32,190,193,195,212,217,274..276,279,307..308,311,
                     313..315)
                     /site_type="other"
                     /note="tetramer (dimer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:133429"
     Site            order(41..43,64,66,69,107..111,145,147,170,174,202,257,
                     261)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:133429"
     Site            order(45,50,72..73,76,78..79,178,180..181,187,253..254,
                     257..260)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:133429"
     Site            order(112,118,147,178,202,247,257)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:133429"
     CDS             1..345
                     /gene="LOC117609252"
                     /coded_by="XM_034335346.2:244..1281"
                     /db_xref="GeneID:117609252"
ORIGIN      
        1 mavpkkklpg tspntatfll tkqpedyvdd hrvkivivgs gytgvavgia ilfkrlasel
       61 vfidanedla kaeaedighv aaflgdpkii gtkdyslare aavcvitvgd kstneeasle
      121 qnlnvfkevi pnvckyapns vllivtspvd ilsyaafkls gfppqrvvgl gtfldscrfq
      181 yfiaqklgls assvqasvig engsssvpiw stvtvrgikl kdinkdigtk tdpeswgdlh
      241 skvidsdsdl iarkgyrcwg igicaaevvd aivrntcici tvstyvkgcr hglekdvyms
      301 lpcvvgregi qtllrhqytp eeqdlmeasc rtiyetqksi lqmle
//
LOCUS       XP_034191251              86 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609259 [Osmia lignaria lignaria].
ACCESSION   XP_034191251
VERSION     XP_034191251.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335360.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..86
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..86
                     /product="uncharacterized protein LOC117609259"
                     /calculated_mol_wt=9375
     CDS             1..86
                     /gene="LOC117609259"
                     /coded_by="XM_034335360.2:272..532"
                     /db_xref="GeneID:117609259"
ORIGIN      
        1 mnslaegatf dsivryvlkh mkitharagy lvmevlkagi avgrikrtpr gtyilasekp
       61 gtiahrirep rfsddsnddv ssdess
//
LOCUS       XP_034172002             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600532 [Osmia lignaria lignaria].
ACCESSION   XP_034172002
VERSION     XP_034172002.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316111.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172002.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC117600532"
                     /calculated_mol_wt=82914
     Region          12..86
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); pfam07776"
                     /db_xref="CDD:462262"
     Region          <284..>407
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     Region          443..482
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          488..510
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          490..510
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(495,497,499,501..502,505..506,509,523,525,529..530,
                     533..534,537,551,553,555,557..558,561..562,565)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          502..527
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          514..>606
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          518..538
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          544..566
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          546..566
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          574..594
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..729
                     /gene="LOC117600532"
                     /coded_by="XM_034316111.2:196..2385"
                     /db_xref="GeneID:117600532"
ORIGIN      
        1 msgyrrvnyy elcrlctsse gnkmnifree grrrqlqtki qtclpiqvle edslpkivch
       61 ecvkklenfi efretvvnae gmlesyftsl rfsedflkeg kvyvkstekd rsatpdneml
      121 ksmekvtssa gnqlvnndqq iqhqqpvvvs nintsniqii sgvqarvqqy kcamqmqpgg
      181 maaavveata ethysyqqqt sqqvspqqsn etqnqindqq spnsqiqqvq sqiqnhnpin
      241 qqiqpqrqis qqpnqtqvsq qqqqqpqnqs qitqqiqqlq rqqreyekqh rqlqqiekqq
      301 vqissqqefp vkqesqtqvv hqnnnivlkv dsnpkqnqqi iqkvqsetqk degtvqnvqs
      361 yttvqgapev flnlgfkdtp lvtqtvreeg kefkdynksd dsmsrnsigq iseflriksg
      421 peptslitvn pqvisqthrd stckgcgkif ssisqlnshs csvqnllset sdgsaikedn
      481 lqtstnsfsc dvcgkpfkrk ehlyqhrklh tgerpyvctt ctkafsrkeh lvrhsvshtg
      541 ekmhdcemcg ksfsrkdnlh khrkthgvsg pyicevcgks fvvkhyysmh laahassaia
      601 ddvncqvplp fkcdichkgf svkqsldthk lrhrpknrnn nnpaqnaang hqpqqqqqqq
      661 qqqpqqqqqt qqsnttnnvn ivtnsgnlns tngqpnsgst vemqsviern eqsngsfnnq
      721 yhsnmqefq
//
LOCUS       XP_034172181             129 aa            linear   INV 03-SEP-2025
DEFINITION  wurmchen 1 transmembrane protein [Osmia lignaria lignaria].
ACCESSION   XP_034172181
VERSION     XP_034172181.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316290.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..129
                     /product="wurmchen 1 transmembrane protein"
                     /calculated_mol_wt=15309
     CDS             1..129
                     /gene="wrm1"
                     /coded_by="XM_034316290.2:238..627"
                     /db_xref="GeneID:117600628"
ORIGIN      
        1 msvlderedy lknpffvkhe ysdftpfyvt vticslifaf lcilnigfcw csrhrsywqs
       61 phtgnrwiqp lftvlphktp pldlselepg rvfesqekqe vlqyhnpksy raapqtqeym
      121 emqkresei
//
LOCUS       XP_034172190             129 aa            linear   INV 03-SEP-2025
DEFINITION  wurmchen 1 transmembrane protein [Osmia lignaria lignaria].
ACCESSION   XP_034172190
VERSION     XP_034172190.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316299.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..129
                     /product="wurmchen 1 transmembrane protein"
                     /calculated_mol_wt=15309
     CDS             1..129
                     /gene="wrm1"
                     /coded_by="XM_034316299.2:237..626"
                     /db_xref="GeneID:117600628"
ORIGIN      
        1 msvlderedy lknpffvkhe ysdftpfyvt vticslifaf lcilnigfcw csrhrsywqs
       61 phtgnrwiqp lftvlphktp pldlselepg rvfesqekqe vlqyhnpksy raapqtqeym
      121 emqkresei
//
LOCUS       XP_034172254              59 aa            linear   INV 03-SEP-2025
DEFINITION  wurmchen 2 transmembrane micropeptide [Osmia lignaria lignaria].
ACCESSION   XP_034172254
VERSION     XP_034172254.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316363.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..59
                     /product="wurmchen 2 transmembrane micropeptide"
                     /calculated_mol_wt=6696
     CDS             1..59
                     /gene="wrm2"
                     /coded_by="XM_034316363.2:1..180"
                     /db_xref="GeneID:117600651"
ORIGIN      
        1 mglhifwpat tcltlfvvci imiilkygsr icklrhtplp sdqeweekay srklsvsma
//
LOCUS       XP_034172099             405 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA:m(4)X modification enzyme TRM13 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034172099
VERSION     XP_034172099.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316208.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172099.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="tRNA:m(4)X modification enzyme TRM13 homolog"
                     /calculated_mol_wt=45890
     Region          3..31
                     /region_name="zf-TRM13_CCCH"
                     /note="CCCH zinc finger in TRM13 protein; pfam11722"
                     /db_xref="CDD:463331"
     Region          49..73
                     /region_name="zf-U11-48K"
                     /note="U11-48K-like CHHC zinc finger; pfam05253"
                     /db_xref="CDD:461603"
     Region          156..400
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..405
                     /gene="LOC117600599"
                     /coded_by="XM_034316208.2:122..1339"
                     /db_xref="GeneID:117600599"
ORIGIN      
        1 menhcmyfvk rkkrycrmtv kqgnkycgeh qeyslestns kdnesvdkri kcpldsthtc
       61 fesklskhlk vcnakrlmda qpsfivkgvn idktcekpqh iplselneli vnavidkvta
      121 ayeklpqfle vvrthkvlsd kindesygkt ikkhmlqnss llshledagl vqdntcfief
      181 gagkgkltyw lgqmiknkkn scivlidrss hrhksdnklk neqsplvikr iradiadlkl
      241 neiteiqkfe ckvgiakhlc gtatdltvsc lvqamqndpk cnvtgliiaf cchhrceyas
      301 yvgknylkqc gftpneftil csiaswatcg yglsnitkts nncrdtrtil tsserenigr
      361 kvkallnwgr leylksvgfq snliyytttd vslenmciva trnqi
//
LOCUS       XP_076545980             543 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600658 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545980
VERSION     XP_076545980.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689865.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..543
                     /product="uncharacterized protein LOC117600658 isoform X1"
                     /calculated_mol_wt=59987
     Region          33..133
                     /region_name="EVH1_WASP-like"
                     /note="WASP family proteins EVH1 domain; cd01205"
                     /db_xref="CDD:269916"
     Site            order(33,35,40,44,61,63,70,72,74,87,99,107,113)
                     /site_type="other"
                     /note="WAS mutation hotspots"
                     /db_xref="CDD:269916"
     Site            order(42,44,52,102,104)
                     /site_type="other"
                     /note="proline-rich peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269916"
     Region          <324..>372
                     /region_name="PLN02983"
                     /note="biotin carboxyl carrier protein of acetyl-CoA
                     carboxylase"
                     /db_xref="CDD:215533"
     Region          443..468
                     /region_name="WH2_WAVE"
                     /note="Wiskott Aldrich syndrome homology region 2 (WH2
                     motif) found in Wiskott-Aldrich Syndrome Protein Family
                     members 1 (WASP1 or WAVE1), 2 (WASP2 or WAVE2) and 3
                     (WASP3 or WAVE3); cd22057"
                     /db_xref="CDD:409200"
     Site            order(444..445,447..449,451..453,458..461)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409200"
     Region          458..461
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409200"
     Region          470..541
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(475..476,478..480,482..484,489..492)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..543
                     /gene="LOC117600658"
                     /coded_by="XM_076689865.1:257..1888"
                     /db_xref="GeneID:117600658"
ORIGIN      
        1 mksgtmdkhr gstllkleen eqvfqlignr cqclaagviq lyltepplhk ewikkttgii
       61 tlirdnprrs fflrlyclqr kamlwehevy namdykaplt yfhtfeaedc maafnfaset
      121 davtlrnill gklnakrqrr qqrrskiege gqhgatlpwr nqsntspaaf stgstsnlln
      181 gtpatidvnr sassssiykt kkkkrdkakk kltkddigpp snfrhvmgwn snnkgmdlep
      241 ldlhlkifld kvgvsehqfr nqdtrnfiyd fieknggmsa iredisssis kgntqqqqsq
      301 qqqhqplqqq qqqppvappr qeypppvpar tiphqtrsap plppsrlnap stppnaplta
      361 ppvhrtlpsr pppvsltaap slppppppap papippsrnn stapvppppp lpnlintneg
      421 tvntsatnnn nhsnnnnnee sadprsmlmq sirsgttlrk vdkpetkpap avggprnall
      481 eqirqgvelr pvanevkskt tpayqgglas alsralaers saihsesdds tsetseddde
      541 wdd
//
LOCUS       XP_076545981             461 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600658 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545981
VERSION     XP_076545981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689866.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..461
                     /product="uncharacterized protein LOC117600658 isoform X2"
                     /calculated_mol_wt=50444
     Region          <1..51
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          361..386
                     /region_name="WH2_WAVE"
                     /note="Wiskott Aldrich syndrome homology region 2 (WH2
                     motif) found in Wiskott-Aldrich Syndrome Protein Family
                     members 1 (WASP1 or WAVE1), 2 (WASP2 or WAVE2) and 3
                     (WASP3 or WAVE3); cd22057"
                     /db_xref="CDD:409200"
     Site            order(362..363,365..367,369..371,376..379)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409200"
     Region          376..379
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409200"
     Region          388..459
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(393..394,396..398,400..402,407..410)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..461
                     /gene="LOC117600658"
                     /coded_by="XM_076689866.1:545..1930"
                     /db_xref="GeneID:117600658"
ORIGIN      
        1 mlwehevyna mdykapltyf htfeaedcma afnfasetda vtlrnillgk lnakrqrrqq
       61 rrskiegegq hgatlpwrnq sntspaafst gstsnllngt patidvnrsa ssssiyktkk
      121 kkrdkakkkl tkddigppsn frhvmgwnsn nkgmdlepld lhlkifldkv gvsehqfrnq
      181 dtrnfiydfi eknggmsair edisssiskg ntqqqqsqqq qhqplqqqqq qppvapprqe
      241 ypppvparti phqtrsappl ppsrlnapst ppnapltapp vhrtlpsrpp pvsltaapsl
      301 ppppppappa pippsrnnst apvppppplp nlintnegtv ntsatnnnnh snnnnneesa
      361 dprsmlmqsi rsgttlrkvd kpetkpapav ggprnalleq irqgvelrpv anevkskttp
      421 ayqgglasal sralaerssa ihsesddsts etsedddewd d
//
LOCUS       XP_076545982             461 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600658 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545982
VERSION     XP_076545982.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689867.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..461
                     /product="uncharacterized protein LOC117600658 isoform X2"
                     /calculated_mol_wt=50444
     Region          <1..51
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          361..386
                     /region_name="WH2_WAVE"
                     /note="Wiskott Aldrich syndrome homology region 2 (WH2
                     motif) found in Wiskott-Aldrich Syndrome Protein Family
                     members 1 (WASP1 or WAVE1), 2 (WASP2 or WAVE2) and 3
                     (WASP3 or WAVE3); cd22057"
                     /db_xref="CDD:409200"
     Site            order(362..363,365..367,369..371,376..379)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409200"
     Region          376..379
                     /region_name="actin-binding sequence"
                     /note="actin-binding sequence [structural motif]"
                     /db_xref="CDD:409200"
     Region          388..459
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(393..394,396..398,400..402,407..410)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..461
                     /gene="LOC117600658"
                     /coded_by="XM_076689867.1:275..1660"
                     /db_xref="GeneID:117600658"
ORIGIN      
        1 mlwehevyna mdykapltyf htfeaedcma afnfasetda vtlrnillgk lnakrqrrqq
       61 rrskiegegq hgatlpwrnq sntspaafst gstsnllngt patidvnrsa ssssiyktkk
      121 kkrdkakkkl tkddigppsn frhvmgwnsn nkgmdlepld lhlkifldkv gvsehqfrnq
      181 dtrnfiydfi eknggmsair edisssiskg ntqqqqsqqq qhqplqqqqq qppvapprqe
      241 ypppvparti phqtrsappl ppsrlnapst ppnapltapp vhrtlpsrpp pvsltaapsl
      301 ppppppappa pippsrnnst apvppppplp nlintnegtv ntsatnnnnh snnnnneesa
      361 dprsmlmqsi rsgttlrkvd kpetkpapav ggprnalleq irqgvelrpv anevkskttp
      421 ayqgglasal sralaerssa ihsesddsts etsedddewd d
//
LOCUS       XP_034172314             161 aa            linear   INV 03-SEP-2025
DEFINITION  ninjurin-A [Osmia lignaria lignaria].
ACCESSION   XP_034172314
VERSION     XP_034172314.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316423.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172314.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="ninjurin-A"
                     /calculated_mol_wt=17743
     Region          42..142
                     /region_name="Ninjurin"
                     /note="pfam04923"
                     /db_xref="CDD:461482"
     CDS             1..161
                     /gene="LOC117600680"
                     /coded_by="XM_034316423.2:1..486"
                     /db_xref="GeneID:117600680"
ORIGIN      
        1 meeekeidpk levqmieevv ikdsvpsvdt tdkksqaykq sntyaakktv aqgmmdvali
       61 tananqlryl ieyqrysptf liilsliiis lmlqiavgvs lifkgrfdik gqskslnarr
      121 innyvvvgvf litiinvfia afsisvpsts pqliqtigng v
//
LOCUS       XP_076545983             413 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305633 [Osmia lignaria lignaria].
ACCESSION   XP_076545983
VERSION     XP_076545983.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689868.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..413
                     /product="uncharacterized protein LOC143305633"
                     /calculated_mol_wt=46664
     Region          <87..>265
                     /region_name="Baculo_F"
                     /note="Baculovirus F protein; pfam12259"
                     /db_xref="CDD:371998"
     CDS             1..413
                     /gene="LOC143305633"
                     /coded_by="XM_076689868.1:236..1477"
                     /db_xref="GeneID:143305633"
ORIGIN      
        1 mmalsrfvhg ieisldhyik taetylavir avksgevphe lldrsisrri isdirntqrn
       61 fdlpippghq slkeltgstt vnaiiyqgri iivlqlplsd gttyhlyrty swpipqrlgq
      121 dttasgyiqp kspylaisnd hrfyfeades yinnchktpy thlcplgrpl seiasstace
      181 vtlllrptth afktcevrlq pqhtpfwahl rtmqawlysl ekpesvqlic ngmkddiiti
      241 egtgifqlra gciaktthet ligmqhlesl eqyfyqpnll lnisefspqi hrykeikplt
      301 mpskasgvst piddsltdie aklaemdthh rsrnlttnvl hgglaylail tiailvyctr
      361 httkkiwitk tcqkrrtepe nqstppeqpp tttppsiaqq plpplpqqra lvn
//
LOCUS       XP_034180533             288 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604310 [Osmia lignaria lignaria].
ACCESSION   XP_034180533
VERSION     XP_034180533.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324642.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="uncharacterized protein LOC117604310"
                     /calculated_mol_wt=32056
     Region          31..131
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     CDS             1..288
                     /gene="LOC117604310"
                     /coded_by="XM_034324642.2:525..1391"
                     /db_xref="GeneID:117604310"
ORIGIN      
        1 mtrhlwlsvt lvflvmsgas clrdvslevv pevvqrgqka ilrchydlek aplyslkwyr
       61 gnsefyrftp seepttmifn itgihvdidn snksqvtlrd idfavsgtfs ceitadaptf
      121 stasgeknlt vvslpegtpi ivsererydp gdtlrancsl ppsepaahls ftlnnvpvds
      181 ytkhqpqqyp qqkwerndap apradqlenl qwieirvklq pfhysngqln lrcsaqipgi
      241 ysaisevqlg aglrepvper vtsdngseas tstclmiltv ctvqlllr
//
LOCUS       XP_034192561             481 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609912 [Osmia lignaria lignaria].
ACCESSION   XP_034192561
VERSION     XP_034192561.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336670.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192561.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="uncharacterized protein LOC117609912"
                     /calculated_mol_wt=54228
     Region          169..316
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..481
                     /gene="LOC117609912"
                     /coded_by="XM_034336670.2:3175..4620"
                     /db_xref="GeneID:117609912"
ORIGIN      
        1 maqllrttdk wseiqsddsq etlevselnp sprtssvlhs hatraslpat lpielqlkvr
       61 tqmgrltsiq dvheavikns gdlsmeelad lkaqieealk afnqehayle vvwptsmahh
      121 eyfeanchlq mqracsavkr fiakekasll nlrstqqlet lpvislpkft gsytdwppff
      181 elfssmiltn trlqnvekfy ylkrclegep aqlisnlplt daslqsaleq lrtryenkrl
      241 liqahldqll alpatkhhsa rllnqlltaa ienrnavlnl vdpnqladci lvhqvssrld
      301 gatkkmwess leatneyptf dqltqfatsr vrilerlead qnksaqpqsk qapppeqpaq
      361 parirrtish qavpraspye acdccgrghy ivacsqfrrm sikdrfmcvq lnrlcynclg
      421 rhplkacktt recklcstrh htmlhgseva fmttstqppa shpkqragss qaysftspga
      481 r
//
LOCUS       XP_034192562             400 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192562
VERSION     XP_034192562.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336671.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="neurotrimin isoform X1"
                     /calculated_mol_wt=44688
     Region          35..125
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          44..48
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          57..61
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          92..96
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          106..111
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          118..121
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          129..219
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          148..152
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          161..165
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          184..188
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          198..203
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          212..215
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          234..316
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          240..244
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          253..257
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          282..287
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          297..302
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     CDS             1..400
                     /gene="LOC117609913"
                     /coded_by="XM_034336671.2:17..1219"
                     /db_xref="GeneID:117609913"
ORIGIN      
        1 mlpilaipvf civawvsaln dplsdlprfg kplnnltvsv greaiftciv enlgpykvaw
       61 lrvdtqtilt ianhvitknh riavthsghr awslhirdtk etdrgwymcq vntdpmssnt
      121 gflevvvppd ildyptstdm vvregsnvtl rcaatgtpep tvtwrreagg titlsnwqev
      181 gqgpeleitr vtrlhmgpyl cvasngvppa vskrivlivh fqpmvwienq lvgayegqrl
      241 tlechseayp rpitywtrpt netiandnny evetipkgye itmrltikav rpqdfgsfrc
      301 vatnslgetd gkiklyridr ppttrrpntr rdskkswkmd hsyekktagm kdesmlkgld
      361 nddeqidfqs atastflhrh lfanlgitia tailagglst
//
LOCUS       XP_034192563             389 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192563
VERSION     XP_034192563.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336672.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="neurotrimin isoform X2"
                     /calculated_mol_wt=43685
     Region          24..114
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          33..37
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          46..50
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          81..85
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          95..100
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          107..110
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          118..208
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          137..141
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          150..154
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          173..177
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          187..192
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          201..204
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          223..305
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          229..233
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          242..246
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          271..276
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          286..291
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     CDS             1..389
                     /gene="LOC117609913"
                     /coded_by="XM_034336672.2:164..1333"
                     /db_xref="GeneID:117609913"
ORIGIN      
        1 mktdvknffh vltdlprfgk plnnltvsvg reaiftcive nlgpykvawl rvdtqtilti
       61 anhvitknhr iavthsghra wslhirdtke tdrgwymcqv ntdpmssntg flevvvppdi
      121 ldyptstdmv vregsnvtlr caatgtpept vtwrreaggt itlsnwqevg qgpeleitrv
      181 trlhmgpylc vasngvppav skrivlivhf qpmvwienql vgayegqrlt lechseaypr
      241 pitywtrptn etiandnnye vetipkgyei tmrltikavr pqdfgsfrcv atnslgetdg
      301 kiklyridrp pttrrpntrr dskkswkmdh syekktagmk desmlkgldn ddeqidfqsa
      361 tastflhrhl fanlgitiat ailagglst
//
LOCUS       XP_034192565             253 aa            linear   INV 03-SEP-2025
DEFINITION  chymotrypsin-2-like isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192565
VERSION     XP_034192565.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336674.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192565.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="chymotrypsin-2-like isoform X1"
                     /calculated_mol_wt=27748
     Region          26..251
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            26
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(68,114,209)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(203,224,226)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..253
                     /gene="LOC117609915"
                     /coded_by="XM_034336674.2:176..937"
                     /db_xref="GeneID:117609915"
ORIGIN      
        1 mllttlslft llslsnagil rfdprivnge nakvgeipyq vslqgkdssf hfcggsilne
       61 nyvitaascv yyttaatikv iagtinltap ksehyvkeii inekynktds wkndiallkv
      121 ekpfvkseqi afvqlpsknq vvkandtavv sgwgrreggp apvhlqrvni liadqeycky
      181 sykdirkvyd sqvcayypsv kkgicngdsg gplivngelt glvswsrgca lteypsvytr
      241 vsaqidwiks hav
//
LOCUS       XP_034192566             203 aa            linear   INV 03-SEP-2025
DEFINITION  mite allergen Eur m 3-like isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192566
VERSION     XP_034192566.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336675.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192566.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..203
                     /product="mite allergen Eur m 3-like isoform X2"
                     /calculated_mol_wt=22041
     Region          26..201
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            26
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(68,114,159)
                     /site_type="active"
                     /db_xref="CDD:238113"
     CDS             1..203
                     /gene="LOC117609915"
                     /coded_by="XM_034336675.2:205..816"
                     /db_xref="GeneID:117609915"
ORIGIN      
        1 mllttlslft llslsnagil rfdprivnge nakvgeipyq vslqgkdssf hfcggsilne
       61 nyvitaascv yyttaatikv iagtinltap ksehyvkeii inekynktds wkndiallkv
      121 ekpfvkseqi afvqlpsknq vvkandtavv sgwgrrgdsg gplivngelt glvswsrgca
      181 lteypsvytr vsaqidwiks hav
//
LOCUS       XP_034192568             113 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609916 [Osmia lignaria lignaria].
ACCESSION   XP_034192568
VERSION     XP_034192568.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336677.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..113
                     /product="uncharacterized protein LOC117609916"
                     /calculated_mol_wt=13458
     CDS             1..113
                     /gene="LOC117609916"
                     /coded_by="XM_034336677.2:4792..5133"
                     /db_xref="GeneID:117609916"
ORIGIN      
        1 mprrrervaa aimlllnkkk krsvwvrqwi srrpefgcfh nllrelqied gqqyfnfirm
       61 tavdveilmq kvghiikked trmrdsispk erliltlrfl asgesyhslm hhi
//
LOCUS       XP_034173836             826 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601298 [Osmia lignaria lignaria].
ACCESSION   XP_034173836
VERSION     XP_034173836.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317945.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173836.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..826
                     /product="uncharacterized protein LOC117601298"
                     /calculated_mol_wt=92154
     Region          631..729
                     /region_name="SH2_SOCS7"
                     /note="Src homology 2 (SH2) domain found in suppressor of
                     cytokine signaling (SOCS) proteins; cd10388"
                     /db_xref="CDD:198251"
     Site            order(649,667,669,677,690)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198251"
     Site            order(700,702,718)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198251"
     Region          752..800
                     /region_name="SOCS_SOCS7"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS7-like proteins. Together with CIS1, the CIS/SOCS
                     family of proteins is characterized by the presence of a
                     C-terminal SOCS box and a central SH2 domain. SOCS7 is
                     important in the functioning of neuronal...; cd03741"
                     /db_xref="CDD:239710"
     Site            order(753..758,764,771,777,782)
                     /site_type="other"
                     /note="putative elongin B/C interaction [polypeptide
                     binding]"
                     /db_xref="CDD:239710"
     CDS             1..826
                     /gene="LOC117601298"
                     /coded_by="XM_034317945.2:864..3344"
                     /db_xref="GeneID:117601298"
ORIGIN      
        1 mdsppscsls atgplsdlgs sgiggsissd svrahlaiem qesdvesksl sqdsfdysdg
       61 mcgenfntlk kgpgwtdadg klevevvdva ikpppefqds psppnsesss apilsyaqlm
      121 rqkvpqlidd yaetlvqsti eealiiscri swpegriapa tnhipvvtrs vynskrlwat
      181 dlltpsscqg attlitpynt lnrpnsrcsl assrlssshn sintsglshk addssfitsa
      241 mshdvlttse isdmynvpfd sdiytvpidv vrplhylrtp qrpkrhrhhr krrrnvsass
      301 qseleahiqq arhycgkpra ithviksqrl lsdvccneag ngkrhsvpgt sgrhasrtsn
      361 ghmhnigepi hmtlhevrqy lqtlyssssd ssenkikrdk vpishtpvhl ampkgisvnn
      421 nnrysnphtd samdipisnk nsnglihnnn nnnnnnnnnn ngnvkkhkkn sfvsikhkkv
      481 keephkekvd serekmkkgq rnfslnlkqt lcnifrfrrl gspdhfvekr nsivqgeive
      541 eeegvdsdqh annnnttsev dpsvqklpff kralpplpks nghvggveqa edalttmvsk
      601 cesptsippq mpppapkvee eptedtsmdf aasiekvkdy gwywgpisge aaekilsnep
      661 dgsfivrdss ddhyifslsf rlnscvrhvr iehdqgnfsf gsctkfksht ivdfienave
      721 hsrsgrylff lhrrpvlgpm rvqllhpvsr fkqvqslqhm crfvilkivr rdliptlplp
      781 rrlidylsmp hyyseqlaeq ddraespvss stgelekicf tpqgls
//
LOCUS       XP_034173912             826 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601298 [Osmia lignaria lignaria].
ACCESSION   XP_034173912
VERSION     XP_034173912.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173912.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..826
                     /product="uncharacterized protein LOC117601298"
                     /calculated_mol_wt=92154
     Region          631..729
                     /region_name="SH2_SOCS7"
                     /note="Src homology 2 (SH2) domain found in suppressor of
                     cytokine signaling (SOCS) proteins; cd10388"
                     /db_xref="CDD:198251"
     Site            order(649,667,669,677,690)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198251"
     Site            order(700,702,718)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198251"
     Region          752..800
                     /region_name="SOCS_SOCS7"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS7-like proteins. Together with CIS1, the CIS/SOCS
                     family of proteins is characterized by the presence of a
                     C-terminal SOCS box and a central SH2 domain. SOCS7 is
                     important in the functioning of neuronal...; cd03741"
                     /db_xref="CDD:239710"
     Site            order(753..758,764,771,777,782)
                     /site_type="other"
                     /note="putative elongin B/C interaction [polypeptide
                     binding]"
                     /db_xref="CDD:239710"
     CDS             1..826
                     /gene="LOC117601298"
                     /coded_by="XM_034318021.2:660..3140"
                     /db_xref="GeneID:117601298"
ORIGIN      
        1 mdsppscsls atgplsdlgs sgiggsissd svrahlaiem qesdvesksl sqdsfdysdg
       61 mcgenfntlk kgpgwtdadg klevevvdva ikpppefqds psppnsesss apilsyaqlm
      121 rqkvpqlidd yaetlvqsti eealiiscri swpegriapa tnhipvvtrs vynskrlwat
      181 dlltpsscqg attlitpynt lnrpnsrcsl assrlssshn sintsglshk addssfitsa
      241 mshdvlttse isdmynvpfd sdiytvpidv vrplhylrtp qrpkrhrhhr krrrnvsass
      301 qseleahiqq arhycgkpra ithviksqrl lsdvccneag ngkrhsvpgt sgrhasrtsn
      361 ghmhnigepi hmtlhevrqy lqtlyssssd ssenkikrdk vpishtpvhl ampkgisvnn
      421 nnrysnphtd samdipisnk nsnglihnnn nnnnnnnnnn ngnvkkhkkn sfvsikhkkv
      481 keephkekvd serekmkkgq rnfslnlkqt lcnifrfrrl gspdhfvekr nsivqgeive
      541 eeegvdsdqh annnnttsev dpsvqklpff kralpplpks nghvggveqa edalttmvsk
      601 cesptsippq mpppapkvee eptedtsmdf aasiekvkdy gwywgpisge aaekilsnep
      661 dgsfivrdss ddhyifslsf rlnscvrhvr iehdqgnfsf gsctkfksht ivdfienave
      721 hsrsgrylff lhrrpvlgpm rvqllhpvsr fkqvqslqhm crfvilkivr rdliptlplp
      781 rrlidylsmp hyyseqlaeq ddraespvss stgelekicf tpqgls
//
LOCUS       XP_034173989             826 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601298 [Osmia lignaria lignaria].
ACCESSION   XP_034173989
VERSION     XP_034173989.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173989.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..826
                     /product="uncharacterized protein LOC117601298"
                     /calculated_mol_wt=92154
     Region          631..729
                     /region_name="SH2_SOCS7"
                     /note="Src homology 2 (SH2) domain found in suppressor of
                     cytokine signaling (SOCS) proteins; cd10388"
                     /db_xref="CDD:198251"
     Site            order(649,667,669,677,690)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198251"
     Site            order(700,702,718)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198251"
     Region          752..800
                     /region_name="SOCS_SOCS7"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS7-like proteins. Together with CIS1, the CIS/SOCS
                     family of proteins is characterized by the presence of a
                     C-terminal SOCS box and a central SH2 domain. SOCS7 is
                     important in the functioning of neuronal...; cd03741"
                     /db_xref="CDD:239710"
     Site            order(753..758,764,771,777,782)
                     /site_type="other"
                     /note="putative elongin B/C interaction [polypeptide
                     binding]"
                     /db_xref="CDD:239710"
     CDS             1..826
                     /gene="LOC117601298"
                     /coded_by="XM_034318098.2:366..2846"
                     /db_xref="GeneID:117601298"
ORIGIN      
        1 mdsppscsls atgplsdlgs sgiggsissd svrahlaiem qesdvesksl sqdsfdysdg
       61 mcgenfntlk kgpgwtdadg klevevvdva ikpppefqds psppnsesss apilsyaqlm
      121 rqkvpqlidd yaetlvqsti eealiiscri swpegriapa tnhipvvtrs vynskrlwat
      181 dlltpsscqg attlitpynt lnrpnsrcsl assrlssshn sintsglshk addssfitsa
      241 mshdvlttse isdmynvpfd sdiytvpidv vrplhylrtp qrpkrhrhhr krrrnvsass
      301 qseleahiqq arhycgkpra ithviksqrl lsdvccneag ngkrhsvpgt sgrhasrtsn
      361 ghmhnigepi hmtlhevrqy lqtlyssssd ssenkikrdk vpishtpvhl ampkgisvnn
      421 nnrysnphtd samdipisnk nsnglihnnn nnnnnnnnnn ngnvkkhkkn sfvsikhkkv
      481 keephkekvd serekmkkgq rnfslnlkqt lcnifrfrrl gspdhfvekr nsivqgeive
      541 eeegvdsdqh annnnttsev dpsvqklpff kralpplpks nghvggveqa edalttmvsk
      601 cesptsippq mpppapkvee eptedtsmdf aasiekvkdy gwywgpisge aaekilsnep
      661 dgsfivrdss ddhyifslsf rlnscvrhvr iehdqgnfsf gsctkfksht ivdfienave
      721 hsrsgrylff lhrrpvlgpm rvqllhpvsr fkqvqslqhm crfvilkivr rdliptlplp
      781 rrlidylsmp hyyseqlaeq ddraespvss stgelekicf tpqgls
//
LOCUS       XP_034174147             354 aa            linear   INV 03-SEP-2025
DEFINITION  S-adenosylmethionine sensor upstream of TORC1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174147
VERSION     XP_034174147.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318256.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174147.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..354
                     /product="S-adenosylmethionine sensor upstream of TORC1
                     isoform X1"
                     /calculated_mol_wt=41192
     Region          <145..297
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..354
                     /gene="Samtor"
                     /coded_by="XM_034318256.2:11..1075"
                     /db_xref="GeneID:117601474"
ORIGIN      
        1 mateehkyla gvikkthatl rkeaqdhgpe iawnrhitrk dvlqeyahsm qklattywmk
       61 nnlntessth sriewikfqc qeyflnggse kynnreesir ikintnnles tevpihynss
      121 ienkespipk yldnntdffa qkialldvgs cynpfgsfni fevtaidlyg ipdkvlgcdf
      181 lnvhigkeki lsdnkeevll lpqnsfnvvv fslfleylpc pkqryicckk aydllqvggi
      241 lfiitpdskh vhanaklmks wryvlsklgf mrikyeklrh mhcivfrkci fkdvatrwan
      301 lqnlssddpl yfdstaiyip qdfrnesdei khqeqeeyds deitsmfgel pfdp
//
LOCUS       XP_034174242             327 aa            linear   INV 03-SEP-2025
DEFINITION  S-adenosylmethionine sensor upstream of TORC1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174242
VERSION     XP_034174242.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318351.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174242.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="S-adenosylmethionine sensor upstream of TORC1
                     isoform X2"
                     /calculated_mol_wt=37796
     Region          <161..247
                     /region_name="UbiG"
                     /note="2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,
                     4-benzoquinol methylase [Coenzyme transport and
                     metabolism]; COG2227"
                     /db_xref="CDD:441829"
     CDS             1..327
                     /gene="Samtor"
                     /coded_by="XM_034318351.2:11..994"
                     /db_xref="GeneID:117601474"
ORIGIN      
        1 mateehkyla gvikkthatl rkeaqdhgpe iawnrhitrk dvlqeyahsm qklattywmk
       61 nnlntessth sriewikfqc qeyflnggse kynnreesir ikintnnles tevpihynss
      121 ienkespipk yldnntdffa qkialldvgs cynpfgsfni fevtaidlyg ipdkvlgcdf
      181 lnvhigkeki lsdnkeevll lpqnsfnvvv fslfleylpc pkqryicckk aydllqvggi
      241 lfiitpdskh vhanaklmks wryvlnvatr wanlqnlssd dplyfdstai yipqdfrnes
      301 deikhqeqee ydsdeitsmf gelpfdp
//
LOCUS       XP_034174372             171 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin conjugating enzyme 7 [Osmia lignaria lignaria].
ACCESSION   XP_034174372
VERSION     XP_034174372.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318481.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="ubiquitin conjugating enzyme 7"
                     /calculated_mol_wt=18908
     Region          10..167
                     /region_name="UBCc_UBE2G2"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 G2 and related
                     proteins; cd23796"
                     /db_xref="CDD:467416"
     CDS             1..171
                     /gene="Ubc7"
                     /coded_by="XM_034318481.2:87..602"
                     /db_xref="GeneID:117601562"
ORIGIN      
        1 miktmagsal rrlmaeykql tlnppegiia gpineenffe wealitgpeg tcfeggvfpa
       61 klifppdypl sppkmqftce mfhpniyadg rvcisilhap gddpmgyess aerwspvqsv
      121 ekillsvvsm laepndesga nvdaakmwre nrpeferiaq klvrktlgip p
//
LOCUS       XP_034174511             143 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L28 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174511
VERSION     XP_034174511.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318620.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="ribosomal protein L28 isoform X1"
                     /calculated_mol_wt=16249
     Region          10..127
                     /region_name="Ribosomal_L28e"
                     /note="Ribosomal L28e protein family; pfam01778"
                     /db_xref="CDD:460323"
     CDS             1..143
                     /gene="RpL28"
                     /coded_by="XM_034318620.2:20..451"
                     /db_xref="GeneID:117601601"
ORIGIN      
        1 mipfkmsshl nwmiirnnna fllkkrnisk pfstepnnlt nlssyrysgl ihrksvgivd
       61 tpdkkgftvv ykkprslkkp akstvrctmk agarrslhkl krlltknkyr vdlskaalrr
      121 asavlrsqkp lpakktrttk kad
//
LOCUS       XP_034174762             143 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L28 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174762
VERSION     XP_034174762.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318871.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="ribosomal protein L28 isoform X1"
                     /calculated_mol_wt=16249
     Region          10..127
                     /region_name="Ribosomal_L28e"
                     /note="Ribosomal L28e protein family; pfam01778"
                     /db_xref="CDD:460323"
     CDS             1..143
                     /gene="RpL28"
                     /coded_by="XM_034318871.2:20..451"
                     /db_xref="GeneID:117601601"
ORIGIN      
        1 mipfkmsshl nwmiirnnna fllkkrnisk pfstepnnlt nlssyrysgl ihrksvgivd
       61 tpdkkgftvv ykkprslkkp akstvrctmk agarrslhkl krlltknkyr vdlskaalrr
      121 asavlrsqkp lpakktrttk kad
//
LOCUS       XP_034174596             143 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L28 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174596
VERSION     XP_034174596.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318705.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="ribosomal protein L28 isoform X1"
                     /calculated_mol_wt=16249
     Region          10..127
                     /region_name="Ribosomal_L28e"
                     /note="Ribosomal L28e protein family; pfam01778"
                     /db_xref="CDD:460323"
     CDS             1..143
                     /gene="RpL28"
                     /coded_by="XM_034318705.2:20..451"
                     /db_xref="GeneID:117601601"
ORIGIN      
        1 mipfkmsshl nwmiirnnna fllkkrnisk pfstepnnlt nlssyrysgl ihrksvgivd
       61 tpdkkgftvv ykkprslkkp akstvrctmk agarrslhkl krlltknkyr vdlskaalrr
      121 asavlrsqkp lpakktrttk kad
//
LOCUS       XP_034174834             143 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L28 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174834
VERSION     XP_034174834.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318943.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="ribosomal protein L28 isoform X1"
                     /calculated_mol_wt=16249
     Region          10..127
                     /region_name="Ribosomal_L28e"
                     /note="Ribosomal L28e protein family; pfam01778"
                     /db_xref="CDD:460323"
     CDS             1..143
                     /gene="RpL28"
                     /coded_by="XM_034318943.2:20..451"
                     /db_xref="GeneID:117601601"
ORIGIN      
        1 mipfkmsshl nwmiirnnna fllkkrnisk pfstepnnlt nlssyrysgl ihrksvgivd
       61 tpdkkgftvv ykkprslkkp akstvrctmk agarrslhkl krlltknkyr vdlskaalrr
      121 asavlrsqkp lpakktrttk kad
//
LOCUS       XP_034174898             143 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L28 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174898
VERSION     XP_034174898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319007.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="ribosomal protein L28 isoform X1"
                     /calculated_mol_wt=16249
     Region          10..127
                     /region_name="Ribosomal_L28e"
                     /note="Ribosomal L28e protein family; pfam01778"
                     /db_xref="CDD:460323"
     CDS             1..143
                     /gene="RpL28"
                     /coded_by="XM_034319007.2:20..451"
                     /db_xref="GeneID:117601601"
ORIGIN      
        1 mipfkmsshl nwmiirnnna fllkkrnisk pfstepnnlt nlssyrysgl ihrksvgivd
       61 tpdkkgftvv ykkprslkkp akstvrctmk agarrslhkl krlltknkyr vdlskaalrr
      121 asavlrsqkp lpakktrttk kad
//
LOCUS       XP_034174985             138 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L28 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034174985
VERSION     XP_034174985.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319094.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..138
                     /product="ribosomal protein L28 isoform X2"
                     /calculated_mol_wt=15633
     Region          5..122
                     /region_name="Ribosomal_L28e"
                     /note="Ribosomal L28e protein family; pfam01778"
                     /db_xref="CDD:460323"
     CDS             1..138
                     /gene="RpL28"
                     /coded_by="XM_034319094.2:153..569"
                     /db_xref="GeneID:117601601"
ORIGIN      
        1 msshlnwmii rnnnafllkk rniskpfste pnnltnlssy rysglihrks vgivdtpdkk
       61 gftvvykkpr slkkpakstv rctmkagarr slhklkrllt knkyrvdlsk aalrrasavl
      121 rsqkplpakk trttkkad
//
LOCUS       XP_034173219            2460 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat kinase [Osmia lignaria lignaria].
ACCESSION   XP_034173219
VERSION     XP_034173219.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317328.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173219.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2460
                     /product="leucine-rich repeat kinase"
                     /calculated_mol_wt=277934
     Region          8..275
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          16..44
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          47..78
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(48,52..53,56..58,60..61,65,68,78,80,84,88..89,
                     92..94,96..97,101,104,113,115,117,121..122,125..127,
                     129..130,134)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          80..113
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          179..489
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(188,192..193,196..198,200..201,205,208,219,221,
                     225..226,229..231,233..234,238,241,251,253,255,259..260,
                     263..265,267..268,272,275)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          188..219
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          221..251
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          307..379
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..432
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          434..461
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <573..>685
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          573..595
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          596..623
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          624..646
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          647..669
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          670..683
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          733..765
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <766..>998
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          766..789
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          790..812
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          888..938
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          914..935
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          939..961
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          962..986
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          1030..1229
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            1037..1044
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206741"
     Site            order(1039..1045,1119,1174..1175,1177,1215..1217)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206741"
     Site            1087
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206741"
     Site            1091..1093
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206741"
     Site            1116..1119
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206741"
     Site            order(1118..1119,1135..1136)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206741"
     Site            1174..1177
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206741"
     Site            1215..1217
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206741"
     Region          1251..>1408
                     /region_name="COR"
                     /note="C-terminal of Roc, COR, domain; pfam16095"
                     /db_xref="CDD:406489"
     Region          1793..2086
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(1793..1796,1799,1801,1819,1821,1878,1892..1895,1942,
                     1946..1947,1949,1970..1971)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..2460
                     /gene="Lrrk"
                     /coded_by="XM_034317328.2:906..8288"
                     /db_xref="GeneID:117601044"
ORIGIN      
        1 medytpided flgrllhqaa lwdnaelled llrgehaqyi nsqdswgrtp lhaaaitens
       61 rcldvllsag anpnipcgpr ghyrtplhvc aehghnsnie kflkfnanlm iqdnngntpl
      121 dvaeknkqda cinllkvaae kyelaklath aslraiciqg divaarniiq gltadvesii
      181 nmapngantl lfiacemghk divrllldhg adcrihpvtk ycplyiacyn gkveivelll
      241 rhfpkqvqsl tvekwlpiha aainghhmii dlllkfeypq htyqrykdss gefeyempfd
      301 intqdvtgqn vlyissmlgn ikivelllny rvkarkikee emgvtqplpi skrkissgiq
      361 rlmsslnfrs ktfdkkddnm icplnldlyc nnatetalha avkgkhtdvv talllagadp
      421 nllvkvpydq ndsernyssa livacqqrdi kiadlllkhg avdntckaik iaaqnrdepl
      481 takllsikay pdceykinkk amtectqtsh fsalssfgnv tystifpntp tminwhdqqc
      541 hlsqirsqwl tdavlhvnkk lsaknndlvl yaitridish nsitsipsti fylqslrcln
      601 maqnkidtls aeaknakdkl cpvleemylq dnrleqlpef imnlpsleim dvsnnklqcl
      661 pdnlwrapkl kelnasfnll kdlpsqvsqn isrksqrddc lnnspssrsl aaqfaisred
      721 slefesftki anaqiidmer phvwtksvqv sekisddneg gvrsvlasln lahnlfnsip
      781 valpclavnl vrlnmaynsl rsmshitsyp aslkqldlsn nqisrwpslp qvdgadlteq
      841 antacycpat nvqspivpgn nrqtatslrd vvlmsvcthr rhlrlenlrt lilannmlnr
      901 iqltsvddge mpnfeeesid kdtkitysgs klyllfpnvs mldvsnnklk dipqniyeln
      961 nlsvlnissn pdivelppqm gllsrlwnln tqgcrlqepl ktmieskkyk tmdvvgylks
     1021 iledakpyar lklmivgiqg igktslleql rqegevpnkk kasehwakrm gnkninakta
     1081 rgttistvgv digdwiyekk vrgqsshgpv yfrtwdfggq reyyathqyf lskrslylvv
     1141 writdgfkgv seifqwlvni qsrapnspvi ivgthydisy ehsealqqyi rdkfinvvda
     1201 ekcglpkvme tievscktrh nikmlcnliy dvvfslrspg skellleqkv pasylaledv
     1261 viqlahdkkl sgadpvlkad qyyaavnnel qklhrsfrdp aelhqatlfl hengiilhyd
     1321 datlkdlyfl dpqwlcdmla hvvtireinp farsgimkld diqhvfksst issidtqgyi
     1381 vsllnkfeva ltwdyrtlli psllpteedi lrnnqmikip vktrgwhvrs nkitspmmtf
     1441 qnfsgdkltt ecvltsrsqp dcsvtrlllm syfpsgfwsr litriladda iveivmsfla
     1501 pfkdfvddni ftsllntqae wvlwqtgiel ryanitllrl kevnynlknv pydyrqfkfk
     1561 lkqdgiwctv dlknsailei wfpvdtlvik qpimsdsvee epmgyqaivv eprpesmpql
     1621 lalivdhidi lledwyptlg trfvhtsegk llvtrlipcp rcllnngene neindsdrla
     1681 ediqryyink erqsqdsyks dgdsgvgyds ltssrmpsle ghpdvakqns nsesilaysw
     1741 mveecilsay snkpiscpkh fdiplshvap diifmdlgsk hliksedikl gkllgrgafg
     1801 fvfkgvcrlp gtyqkvdvai kmlqpvppgs nskqsavlay kaaqskwdrd plqyackayc
     1861 tarqelnill tlrhinivpl igivisplal vldlapegal dsvlknyrrs gakldpytlq
     1921 aiilqvakav eylhqqhviy rdlksenvlv wqmplpfqnh pehpvhvkva dygisrltlp
     1981 tgakgfggte gfmapeiiky ngeeeytekv dsfsfgmfiy elitlrqpfe gheavkecil
     2041 eggrppltyr etfhpcyald lmvicwaqnp kerptasqiv siasapefth lidvillter
     2101 tqvtaitmtn ktieeningd eiwfghnnge vdmllgtqkg wlqhvrietp iipyamcgvd
     2161 gyiwigdntg qvhvylgsnf gcvasynlep nhsieskvig liyleqlkqf avalhsgktf
     2221 llsnsftqmk kmeimtniqd niktyslaav hkkkrilelw vgqsfgrisi yvlkdsslid
     2281 tvqichvsge vavknlfaty llsaensils ytypgcivyq wdvdtrqiin kldmsklvpc
     2341 seslksisie enlstekcqv taletcrnql yigttwgciv vaecnslrpi tvfrpfegkv
     2401 cqiisfksmd ekklvlatvg qgyrslisry tdfpidnlds ddlkhsmyal lwrsehwsaa
//
LOCUS       XP_034173444             305 aa            linear   INV 03-SEP-2025
DEFINITION  deoxyhypusine hydroxylase nero [Osmia lignaria lignaria].
ACCESSION   XP_034173444
VERSION     XP_034173444.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317553.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..305
                     /product="deoxyhypusine hydroxylase nero"
                     /calculated_mol_wt=34149
     Region          <26..131
                     /region_name="HEAT"
                     /note="HEAT repeat [General function prediction only];
                     COG1413"
                     /db_xref="CDD:441023"
     Region          41..69
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          71..103
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          104..130
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          164..283
                     /region_name="HEAT"
                     /note="HEAT repeat [General function prediction only];
                     COG1413"
                     /db_xref="CDD:441023"
     Region          164..190
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(182..183,186,189..190,213..214,217,220..221,224,
                     246..247,250,253..254,257,277..278,281,284..285)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          199..224
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          259..285
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..305
                     /gene="nero"
                     /coded_by="XM_034317553.2:240..1157"
                     /db_xref="GeneID:117601125"
ORIGIN      
        1 mleisesqis aighvlndqt rplkerfral ftlkniggvk aieqinncfk desallkhel
       61 ayclgqmqdt raipvlieil kdvkhepmvr heagealgai gdlsiipvle eyskdcvpev
      121 aetcqlalhr lqwlksnnsk dlmnsqrssy msvdpappae itdieklksi llnenaslfe
      181 ryqamfslrn ihtpesilal ceglnagsal frheiafvlg qlqeeisvpy latslenmee
      241 nemvrhecae algsiatsdc fdilnkyind nqrvvresci ialdmceyen ssefqyantl
      301 tkvna
//
LOCUS       XP_034173578             162 aa            linear   INV 03-SEP-2025
DEFINITION  oxidized purine nucleoside triphosphate hydrolase [Osmia lignaria
            lignaria].
ACCESSION   XP_034173578
VERSION     XP_034173578.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..162
                     /product="oxidized purine nucleoside triphosphate
                     hydrolase"
                     /calculated_mol_wt=18986
     Region          6..139
                     /region_name="NUDIX_MTH1_Nudt1"
                     /note="MutT homolog-1 (MTH1); cd03427"
                     /db_xref="CDD:467533"
     CDS             1..162
                     /gene="LOC117601192"
                     /coded_by="XM_034317687.2:378..866"
                     /db_xref="GeneID:117601192"
ORIGIN      
        1 mtakqifslv fvrnttnill gfkkrgfgqd kwngfggkve pgetivqgai relkeecgls
       61 tqdlkrvgil efefegndsl levhvfetyn yqeketesee mrpkwfnlkd ipfkqmwpdd
      121 eywfpymlrg elfkgyflyr gqdliikhni etlkelpamd vp
//
LOCUS       XP_034173709             143 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein S23 [Osmia lignaria lignaria].
ACCESSION   XP_034173709
VERSION     XP_034173709.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317818.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173709.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="mitochondrial ribosomal protein S23"
                     /calculated_mol_wt=16857
     Region          2..121
                     /region_name="MRP-S23"
                     /note="Mitochondrial ribosomal protein S23; pfam10484"
                     /db_xref="CDD:463108"
     CDS             1..143
                     /gene="mRpS23"
                     /coded_by="XM_034317818.2:146..577"
                     /db_xref="GeneID:117601245"
ORIGIN      
        1 mahsrterig tifsrvtalw kskaiteesl plwyniyktf ppkyeprfdr papqksvrni
       61 fyeedivrak fqkdvtlpai dlksdklsqt qlfyiiyanl krisskekee diyikvleiy
      121 ervfgtsvkr renkssdddt kki
//
LOCUS       XP_034187721            1380 aa            linear   INV 03-SEP-2025
DEFINITION  DNA repair protein rad50 [Osmia lignaria lignaria].
ACCESSION   XP_034187721
VERSION     XP_034187721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331830.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1380
                     /product="DNA repair protein rad50"
                     /calculated_mol_wt=160541
     Region          2..1310
                     /region_name="rad50"
                     /note="TIGR00606"
                     /db_xref="CDD:129694"
     CDS             1..1380
                     /gene="rad50"
                     /coded_by="XM_034331830.2:317..4459"
                     /db_xref="GeneID:117607753"
ORIGIN      
        1 msrirrlsir girnfgdese eseirfsrpl tlilgpngtg kttiiealkf atcgefppgs
       61 drgkffihdp altttssvrg vikaeisdav gnvfticrti eysksntsmk fktldtalsr
      121 iskitkevvs itnrctnvdt eltlamgvsk pildyvifch qedlnwpfqd gkklkekfde
      181 ifdsarynka lenimkyikd lrqriiilkg qkencsyivk eledketkle dhkkrvknik
      241 ikieeinedl epmsqklnem eqlesdykdl lseekrkkae yqmskeqsqr lqknisqlfk
      301 gslekleeey ksydtnlkkk adeikelets lkgivkeess isklladerv tngslkqqik
      361 eqerkiasrn kllnealsaw dlenvdsdvs eievialskr lheklrelns klgenklqre
      421 ekekeiqkev dtlrseylti dsqknvkeke lietreelnk vktemsqiga aanklnsies
      481 klenvkrkiq elnevmdvda akeevldktk lrnemeirln aidediasll kqsslqaele
      541 lnksallske keieklkekh eqklmkllnv tklpesklkn kldtvqkqlm deiekinqei
      601 qkaehqstts ettithmnhe lqkkkkemes dklkiaticy yknfddvllm qsrkvkdlqd
      661 krgiyahqga aykeymrqlk etnpccplch rdfdkreavv allkdmekem esypsrlkec
      721 eielrteqek ydkmlqlkpv venivrleet eletmksnie tyenklihsq tfvielkekk
      781 sepenklamy neimsdimlw dnyideiykl kqiinniqkr mtdagiqtky sleeaqakre
      841 elkaslknls keietlqtki nthgekinna reeqntlhae klkiesnmqk lkqlkdkree
      901 lfskeislgs lldqlrkkva aaeselnaav helekskken weklendrkl steadrrlad
      961 ltkaqeetdl fiyrkipksi esseskitky qksidellek kndiesrvnk lkqdincqei
     1021 rkrelydnil llknqevtkk lekecsiiev klsnihypri veerkqlqnr eqtllrqknm
     1081 ikgnqeeler avkecteelr kdiyrqarkn yktkcieltv leetiinlne yskvldaami
     1141 eyheermatv nriikemwrl vytgtdttsi eirtdategi gsgkrtynyk lvqtkhghei
     1201 dmkgrcsagq kvlasiiirl alaetfcknc gvlaldeptt nldqenadsl asalamvvkl
     1261 rsqhqknfql ivishdekfl lklaelnnnk gfhelyrkyn gytairhcqv qnrdhaissv
     1321 ikeeessded adessaqikd nsqsgtglhe kgalqkrkrn idnednnerf skkryvlnlt
//
LOCUS       XP_034187747             207 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab7 [Osmia lignaria lignaria].
ACCESSION   XP_034187747
VERSION     XP_034187747.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331856.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..207
                     /product="RAS oncogene family member Rab7"
                     /calculated_mol_wt=23099
     Region          9..179
                     /region_name="Rab7"
                     /note="Rab GTPase family 7 (Rab7); cd01862"
                     /db_xref="CDD:206655"
     Site            9..10
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206655"
     Site            15..22
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206655"
     Site            order(17..23,33,35,40,66,125..126,128,155..157)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206655"
     Site            order(17,42,44..45,63,67,69..72,75..76,79)
                     /site_type="active"
                     /note="GDI interaction site [active]"
                     /db_xref="CDD:206655"
     Site            order(23..34,36..39)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206655"
     Site            order(33,38..46)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206655"
     Site            order(38,42..49,56,58)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206655"
     Site            40
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206655"
     Site            order(41,43..45,60,62,69..70,73,77,79..82,173)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206655"
     Site            41..45
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206655"
     Site            58..62
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206655"
     Site            63..66
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206655"
     Site            order(66,68..78)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206655"
     Site            69..74
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206655"
     Site            77..81
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206655"
     Site            86..91
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206655"
     Site            117..122
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206655"
     Site            125..128
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206655"
     Site            155..157
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206655"
     Site            171..179
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206655"
     CDS             1..207
                     /gene="Rab7"
                     /coded_by="XM_034331856.2:147..770"
                     /db_xref="GeneID:117607764"
ORIGIN      
        1 masrkklllk viilgdsgvg ktslmnqyvs kkfsnqykat igadfltkev rvddrivtmq
       61 iwdtagqerf qslgvafyrg adccvlvfdv sapssfksld swrdefliqa sprdpdnfpf
      121 vvlgnkvdle sravsskraq qwcqsknnip yfetsakeai nveqafqtia knalaqesev
      181 elynefpdqi kltndqrnng kgdscac
//
LOCUS       XP_034187754             207 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab7 [Osmia lignaria lignaria].
ACCESSION   XP_034187754
VERSION     XP_034187754.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331863.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..207
                     /product="RAS oncogene family member Rab7"
                     /calculated_mol_wt=23099
     Region          9..179
                     /region_name="Rab7"
                     /note="Rab GTPase family 7 (Rab7); cd01862"
                     /db_xref="CDD:206655"
     Site            9..10
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:206655"
     Site            15..22
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206655"
     Site            order(17..23,33,35,40,66,125..126,128,155..157)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206655"
     Site            order(17,42,44..45,63,67,69..72,75..76,79)
                     /site_type="active"
                     /note="GDI interaction site [active]"
                     /db_xref="CDD:206655"
     Site            order(23..34,36..39)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:206655"
     Site            order(33,38..46)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206655"
     Site            order(38,42..49,56,58)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206655"
     Site            40
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206655"
     Site            order(41,43..45,60,62,69..70,73,77,79..82,173)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206655"
     Site            41..45
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:206655"
     Site            58..62
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:206655"
     Site            63..66
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206655"
     Site            order(66,68..78)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206655"
     Site            69..74
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:206655"
     Site            77..81
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:206655"
     Site            86..91
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:206655"
     Site            117..122
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:206655"
     Site            125..128
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206655"
     Site            155..157
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206655"
     Site            171..179
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:206655"
     CDS             1..207
                     /gene="Rab7"
                     /coded_by="XM_034331863.2:432..1055"
                     /db_xref="GeneID:117607764"
ORIGIN      
        1 masrkklllk viilgdsgvg ktslmnqyvs kkfsnqykat igadfltkev rvddrivtmq
       61 iwdtagqerf qslgvafyrg adccvlvfdv sapssfksld swrdefliqa sprdpdnfpf
      121 vvlgnkvdle sravsskraq qwcqsknnip yfetsakeai nveqafqtia knalaqesev
      181 elynefpdqi kltndqrnng kgdscac
//
LOCUS       XP_034172870             732 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600895 [Osmia lignaria lignaria].
ACCESSION   XP_034172870
VERSION     XP_034172870.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172870.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..732
                     /product="uncharacterized protein LOC117600895"
                     /calculated_mol_wt=82465
     Region          <326..424
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..732
                     /gene="LOC117600895"
                     /coded_by="XM_034316979.2:153..2351"
                     /db_xref="GeneID:117600895"
ORIGIN      
        1 mekdqpdsla tvavvsekmp hpphsnyaps evyssaeppp aymrpkstav qitriaavtl
       61 vtmsvilgsf ilaaswvqar asctpesiaa mqaelrlqqq qqqqasasyq qgeflkhlqp
      121 ealiqdttel keaqqnlaaq tpvkkevmpd tkdiklqked ennsksdses sdhddddddy
      181 dddefppvhi klplqfdfde iagtliqear srvscvverr radqvmdgan nnvenntdsr
      241 yqrlsgerva ilcesgnphq eqqeqemltp iiiplgtvqv plqsqepnpg yhqyqihtqy
      301 qvpdmqqlpl sdprglnhip pgvlppelrn lpqvtmemrp prvgpqapmm gpqnpmmgpq
      361 npmmgpqnpa mgpqnsapgp qveirripie lhhypvnpir gmrppmpqep qqvpmmyhqv
      421 eqapgafnqe qgpammpppa pqaeaqiheq rviphmlipq teqrppqvpp qvqpqmqpqp
      481 qaqpqpepqp aqvterqrsp fqgipfeirr iiqqvpveik niiqhitgeg rpvpvqvpeg
      541 arrenvqefk pfpegaqpip lglplpievr nllehgsies hqrsdasseq qeaksysgqe
      601 edseeernfp vpaeirniih qvvegnafpv prfslslkpv esleipvear aevsdsqmae
      661 qdqerqpeqq hvthmmiqqq hqpqqtqqqp qqeqeeesrp hyvqprsvrs ipeafthrre
      721 krvrrcacdc ac
//
LOCUS       XP_034173000             324 aa            linear   INV 03-SEP-2025
DEFINITION  nth-like DNA glycosylase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034173000
VERSION     XP_034173000.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317109.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173000.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..324
                     /product="nth-like DNA glycosylase 1"
                     /calculated_mol_wt=36986
     Region          135..315
                     /region_name="Nth"
                     /note="Endonuclease III [Replication, recombination and
                     repair]; COG0177"
                     /db_xref="CDD:439947"
     CDS             1..324
                     /gene="Nthl1"
                     /coded_by="XM_034317109.2:229..1203"
                     /db_xref="GeneID:117600951"
ORIGIN      
        1 mskklkldhl ttskclrsgt kagstdssst tskyfdnkda lpkkvekkkl spikikneee
       61 qnvikvesnd ikvedskhev ikeestqken nwmppnweii wnnvkemrkh etapvdemgc
      121 hkcadpeata kvaryqslva lmlssqtkdq vthaamqrln aygctpeimk dipddvlqkl
      181 iypvgfrkrk aeyikrtari lvdkyngdip rtlkelcelp gvgpkmshic mqiawgevsg
      241 igvdthvhri cnrlewvkkp tktpeetria leqwlpkdlw neinhllvgf gqeiclprfp
      301 kcseclnkdi cpfgkkniik kcdf
//
LOCUS       XP_076545985             536 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545985
VERSION     XP_076545985.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689870.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..536
                     /product="facilitated trehalose transporter Tret1 isoform
                     X1"
                     /calculated_mol_wt=58687
     Region          90..516
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(103..104,107..108,111,143,193..194,196..198,201,221,
                     224..225,228,328,331..332,335..337,340,367,371,432..433,
                     437,441,457,460..461,464..465,468)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..536
                     /gene="LOC117603295"
                     /coded_by="XM_076689870.1:53..1663"
                     /db_xref="GeneID:117603295"
ORIGIN      
        1 mrsrgvcagv ecallgqssn grggaaaclf sccnvasfqa pilwrrrgdh glaesatmte
       61 nviepvviee kavekektee cksrlhlnqi iasfiatltl lscglcigwp tiflhgkesd
      121 vpltpdnrtl vaiavligac agpilsglli drigrkwfly mtttpilagw iliffakkwl
      181 llfmgrlvag lsvgaiytvv piytgeitep rvrgaasgim avmlnlgymf tygvgpilgr
      241 knlavinlip vivfgltfvw ipespyyylk kqkqkdaels ltwlrgklnn ekeieeiktf
      301 ikseekggvk elavptnrka flilmllfag qqlsgllaie sytrvllpki dfdvdirvtl
      361 liissltlvt slittlvvdk igrkvvflis aygaticllv vgvycylngk vaklneltwv
      421 plivillhsi vfsfglgsvp aimsselfpm nvkswaitfa nifsfllali vagcyelvid
      481 afgyyiifwa lgileliiti tltvimpets rksfieiqqt lkgnsnvpsa dktnsp
//
LOCUS       XP_034178302             479 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178302
VERSION     XP_034178302.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322411.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178302.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="facilitated trehalose transporter Tret1 isoform
                     X2"
                     /calculated_mol_wt=52834
     Region          33..459
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(46..47,50..51,54,86,136..137,139..141,144,164,
                     167..168,171,271,274..275,278..280,283,310,314,375..376,
                     380,384,400,403..404,407..408,411)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..479
                     /gene="LOC117603295"
                     /coded_by="XM_034322411.2:147..1586"
                     /db_xref="GeneID:117603295"
ORIGIN      
        1 mtenviepvv ieekavekek teecksrlhl nqiiasfiat ltllscglci gwptiflhgk
       61 esdvpltpdn rtlvaiavli gacagpilsg llidrigrkw flymtttpil agwiliffak
      121 kwlllfmgrl vaglsvgaiy tvvpiytgei teprvrgaas gimavmlnlg ymftygvgpi
      181 lgrknlavin lipvivfglt fvwipespyy ylkkqkqkda elsltwlrgk lnnekeieei
      241 ktfikseekg gvkelavptn rkaflilmll fagqqlsgll aiesytrvll pkidfdvdir
      301 vtlliisslt lvtslittlv vdkigrkvvf lisaygatic llvvgvycyl ngkvaklnel
      361 twvplivill hsivfsfglg svpaimssel fpmnvkswai tfanifsfll alivagcyel
      421 vidafgyyii fwalgileli ititltvimp etsrksfiei qqtlkgnsnv psadktnsp
//
LOCUS       XP_034177884             506 aa            linear   INV 03-SEP-2025
DEFINITION  signal recognition particle 54k [Osmia lignaria lignaria].
ACCESSION   XP_034177884
VERSION     XP_034177884.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..506
                     /product="signal recognition particle 54k"
                     /calculated_mol_wt=55493
     Region          2..428
                     /region_name="SRP54_euk"
                     /note="signal recognition particle protein SRP54;
                     TIGR01425"
                     /db_xref="CDD:273615"
     CDS             1..506
                     /gene="Srp54k"
                     /coded_by="XM_034321993.2:261..1781"
                     /db_xref="GeneID:117603129"
ORIGIN      
        1 mvladlgrki tsalrslsna tvineevlns mlkeicaall eadvnirlvk klrenvrlvi
       61 dfddmaggln krrmiqsavf kelvklidpg vkayqpikgr sniimfvglq gsgktttctk
      121 layhylkknw kaclvcadtf ragaydqikq natkaripfy gsytevdpva iaqdgvemfk
      181 kegyeiiivd tsgrhkqees lfeemlqvan aiqpdniifv mdatigqace aqakafkerv
      241 nvgsiiitkl dghakgggal savaatqspv ifvgtgehid dlepfktkpf iskllgmgdi
      301 eglidkvnel nlddneelle kikhgqftlr dmyeqfqnim kmgpfsqimg mipgfsqdfm
      361 skgteqesva rlkrlmtimd smndaeldnr dgaklfskqp grivrvaqgs gvtekevkdl
      421 itqytkfaav vkkmggikgl fkagdmsknv nstqmaklnh qmakmmdprv lhqmggmpgl
      481 qnimkqlqqg aagglgnlmg gfggks
//
LOCUS       XP_034178103             462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603189 [Osmia lignaria lignaria].
ACCESSION   XP_034178103
VERSION     XP_034178103.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322212.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="uncharacterized protein LOC117603189"
                     /calculated_mol_wt=54377
     CDS             1..462
                     /gene="LOC117603189"
                     /coded_by="XM_034322212.1:85..1473"
                     /db_xref="GeneID:117603189"
ORIGIN      
        1 mkfactiyil ivvfaenkek flvrciahfe eselvhlsen lnrleclqlv kamydltpan
       61 iedersnyev lyqgsnhlst kskeclaele hwnnnfptnt kpngrsamem tlrwlgrpdl
      121 akyvrenhrt vnymdtenyd kadtlefpgh rvsrrhtpkk dkhssktgkk kkktkkrghr
      181 ehpqnsgkkd kgpqkavaat aghatahrsr elnktdnqsq nekdyveqhr siccsilcvl
      241 fflilclvav yflhrrnrlk argtrfkydn svkkenkdtl tdrlewndet vcscsdvedg
      301 ytgkcmmcgg nyqtyhtipr rndshdsmkd sksmpvqkir kkrkrqrfsf lprnfhdrkr
      361 gkdqedrdrk ktlekslmkk rekvsstcyg dyeqclcckc nlsytermlr ekleerrelh
      421 klrakkrkee krrkremqka erhanplfsl gkklaavksv pe
//
LOCUS       XP_034178437             386 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603389 [Osmia lignaria lignaria].
ACCESSION   XP_034178437
VERSION     XP_034178437.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322546.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178437.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="uncharacterized protein LOC117603389"
                     /calculated_mol_wt=45422
     Region          33..163
                     /region_name="Mb-like"
                     /note="myoglobin-like; M family globin domain; cd01040"
                     /db_xref="CDD:381254"
     Site            order(64..65,82,85..86,89,111,114..115,120,124..125,128)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:381254"
     CDS             1..386
                     /gene="LOC117603389"
                     /coded_by="XM_034322546.2:72..1232"
                     /db_xref="GeneID:117603389"
ORIGIN      
        1 mgnipyrllm hlrvrkdhda ispvdykdfe tmkriwqvie tdssyygfaf ctvifknhpq
       61 yakyfedesi pefiqeakvk kkfsiicdml calfvdyhdn psqreyllgy iamvhknmgl
      121 tlkdlenflg dlmetlvmel pnimteecvs vqtkyfhnlt nillirmdeh ekrftkmlns
      181 sdtikkcctw ctgneprviy gfplkywlyk kryweyrkav wvaveadmlr dfqkgsaghr
      241 fkrlffdhkt tetrkvhrkr rtvlasnsse sdiivgrkes fgqmsrksse drsrrisnsp
      301 ykaekdpfrs selefdqdvt yndntenmri vaavvpskve inrkdedmet lssliasgsd
      361 lksqkatsnh tarerkrirn siirln
//
LOCUS       XP_034178562             286 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603454 [Osmia lignaria lignaria].
ACCESSION   XP_034178562
VERSION     XP_034178562.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322671.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="uncharacterized protein LOC117603454"
                     /calculated_mol_wt=32942
     CDS             1..286
                     /gene="LOC117603454"
                     /coded_by="XM_034322671.2:71..931"
                     /db_xref="GeneID:117603454"
ORIGIN      
        1 mnlrivitna lflfslhlnc ifsqiiaeid vpqlqllaan lkpeecvrlv fldshsqlsd
       61 vqiqkltreq scfrrlvkwi cqlrtvtrnt yaivnnfler igrrdliacl aefnmkssra
      121 pkvirnvept dyfedyegit vttskpkdlq vtkttnqysn qtiekisgqt tikmslrtvg
      181 iilvtivllt ccctlfirnr lahlwnkirg kkkkgklipi gtedisrsyf vrkphtkrkr
      241 psyelvhaat qnnivavpke peeppptgcy kcykkkrkks tkrphr
//
LOCUS       XP_034178685             248 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603514 [Osmia lignaria lignaria].
ACCESSION   XP_034178685
VERSION     XP_034178685.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322794.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="uncharacterized protein LOC117603514"
                     /calculated_mol_wt=28594
     CDS             1..248
                     /gene="LOC117603514"
                     /coded_by="XM_034322794.2:105..851"
                     /db_xref="GeneID:117603514"
ORIGIN      
        1 mmkellmmhk wlyfiiilsr ykvlatmdvn ldelkylaar lnpfecrrli aalhyttyel
       61 pntlaaaern vddeipcirh llhwnsspae gkgktheaia hrlrqinrsd ladwlsksaf
      121 mqlgkdlgra vvkafdelsk eetepshpvt lepiqtyree edpwvqidtv lmaillgllg
      181 tlltlicati fkkirdhlrk nkykklaqke tqneeakmee krikyvtess sgitdnvhpd
      241 setetdfn
//
LOCUS       XP_034178816             170 aa            linear   INV 03-SEP-2025
DEFINITION  globin 1 [Osmia lignaria lignaria].
ACCESSION   XP_034178816
VERSION     XP_034178816.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322925.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..170
                     /product="globin 1"
                     /calculated_mol_wt=18940
     Region          31..164
                     /region_name="Mb-like"
                     /note="myoglobin-like; M family globin domain; cd01040"
                     /db_xref="CDD:381254"
     Site            order(63..64,84,87..88,91,112,115..116,121,125..126,129)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:381254"
     CDS             1..170
                     /gene="Glob1"
                     /coded_by="XM_034322925.2:167..679"
                     /db_xref="GeneID:117603564"
ORIGIN      
        1 mgtflrllgi ssddnridqa tgltekqkkl vqntwsvirk devrsgvlvm ttffkkypay
       61 qqhfaafkdi pldalpdnkr fqahcasiia alnnvidslh dpglmeaslt slaerhkkrg
      121 qtkeefenlk gvvlqvlgqa lgrqftpevs eawsktldva fgkiyqvfas
//
LOCUS       XP_034181051             781 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181051
VERSION     XP_034181051.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325160.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="protein zyg-11 homolog B isoform X1"
                     /calculated_mol_wt=87728
     Region          87..>260
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..781
                     /gene="LOC117604496"
                     /coded_by="XM_034325160.2:255..2600"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttcnqqvnf sinsnnidil
       61 getiarasnd gssgsarrls frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr
      121 lrhvqlkdas tlsakglkvl kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg
      181 sfmdcsrycv vvtlsklral htlnvsetef nkhgleivve dlpllesldi sctkvdditp
      241 lkkcknrlkt lnmynlkine sanlisvlle lnelrnldis derdmhalns vlgpakpkit
      301 dflkavhcmp yltsldlsgk gdidpkdlke fikahpylrf lglvstdacf ddslinpsne
      361 dyrpglvvsg tatesqilea lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm
      421 reypqevrvq vpctaclynl tkgefaimih psilkqvvel tmiamesypt nyrlqmntll
      481 tlcsdrilqd vafdkyrcar lvlnslltfn epsmnrmsva icsllaakis tvetsllgae
      541 plymskllqm vkskmesksv ditmkftlsa lwnltdesvt tcevfldegg melclkmles
      601 fqdessvetk vlgllnniae vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll
      661 sdgprawttm itvptrdgll eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql
      721 wavwaihnvc tknpkrycsm lireggveil kylesthteq tmkpnlqslc qsilktleln
      781 s
//
LOCUS       XP_034180534             781 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180534
VERSION     XP_034180534.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324643.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="protein zyg-11 homolog B isoform X1"
                     /calculated_mol_wt=87728
     Region          87..>260
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..781
                     /gene="LOC117604496"
                     /coded_by="XM_034324643.2:258..2603"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttcnqqvnf sinsnnidil
       61 getiarasnd gssgsarrls frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr
      121 lrhvqlkdas tlsakglkvl kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg
      181 sfmdcsrycv vvtlsklral htlnvsetef nkhgleivve dlpllesldi sctkvdditp
      241 lkkcknrlkt lnmynlkine sanlisvlle lnelrnldis derdmhalns vlgpakpkit
      301 dflkavhcmp yltsldlsgk gdidpkdlke fikahpylrf lglvstdacf ddslinpsne
      361 dyrpglvvsg tatesqilea lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm
      421 reypqevrvq vpctaclynl tkgefaimih psilkqvvel tmiamesypt nyrlqmntll
      481 tlcsdrilqd vafdkyrcar lvlnslltfn epsmnrmsva icsllaakis tvetsllgae
      541 plymskllqm vkskmesksv ditmkftlsa lwnltdesvt tcevfldegg melclkmles
      601 fqdessvetk vlgllnniae vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll
      661 sdgprawttm itvptrdgll eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql
      721 wavwaihnvc tknpkrycsm lireggveil kylesthteq tmkpnlqslc qsilktleln
      781 s
//
LOCUS       XP_034180629             781 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180629
VERSION     XP_034180629.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324738.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="protein zyg-11 homolog B isoform X1"
                     /calculated_mol_wt=87728
     Region          87..>260
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..781
                     /gene="LOC117604496"
                     /coded_by="XM_034324738.2:255..2600"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttcnqqvnf sinsnnidil
       61 getiarasnd gssgsarrls frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr
      121 lrhvqlkdas tlsakglkvl kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg
      181 sfmdcsrycv vvtlsklral htlnvsetef nkhgleivve dlpllesldi sctkvdditp
      241 lkkcknrlkt lnmynlkine sanlisvlle lnelrnldis derdmhalns vlgpakpkit
      301 dflkavhcmp yltsldlsgk gdidpkdlke fikahpylrf lglvstdacf ddslinpsne
      361 dyrpglvvsg tatesqilea lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm
      421 reypqevrvq vpctaclynl tkgefaimih psilkqvvel tmiamesypt nyrlqmntll
      481 tlcsdrilqd vafdkyrcar lvlnslltfn epsmnrmsva icsllaakis tvetsllgae
      541 plymskllqm vkskmesksv ditmkftlsa lwnltdesvt tcevfldegg melclkmles
      601 fqdessvetk vlgllnniae vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll
      661 sdgprawttm itvptrdgll eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql
      721 wavwaihnvc tknpkrycsm lireggveil kylesthteq tmkpnlqslc qsilktleln
      781 s
//
LOCUS       XP_034180716             781 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180716
VERSION     XP_034180716.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324825.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="protein zyg-11 homolog B isoform X1"
                     /calculated_mol_wt=87728
     Region          87..>260
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..781
                     /gene="LOC117604496"
                     /coded_by="XM_034324825.2:249..2594"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttcnqqvnf sinsnnidil
       61 getiarasnd gssgsarrls frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr
      121 lrhvqlkdas tlsakglkvl kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg
      181 sfmdcsrycv vvtlsklral htlnvsetef nkhgleivve dlpllesldi sctkvdditp
      241 lkkcknrlkt lnmynlkine sanlisvlle lnelrnldis derdmhalns vlgpakpkit
      301 dflkavhcmp yltsldlsgk gdidpkdlke fikahpylrf lglvstdacf ddslinpsne
      361 dyrpglvvsg tatesqilea lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm
      421 reypqevrvq vpctaclynl tkgefaimih psilkqvvel tmiamesypt nyrlqmntll
      481 tlcsdrilqd vafdkyrcar lvlnslltfn epsmnrmsva icsllaakis tvetsllgae
      541 plymskllqm vkskmesksv ditmkftlsa lwnltdesvt tcevfldegg melclkmles
      601 fqdessvetk vlgllnniae vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll
      661 sdgprawttm itvptrdgll eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql
      721 wavwaihnvc tknpkrycsm lireggveil kylesthteq tmkpnlqslc qsilktleln
      781 s
//
LOCUS       XP_034180802             781 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180802
VERSION     XP_034180802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324911.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="protein zyg-11 homolog B isoform X1"
                     /calculated_mol_wt=87728
     Region          87..>260
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..781
                     /gene="LOC117604496"
                     /coded_by="XM_034324911.2:258..2603"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttcnqqvnf sinsnnidil
       61 getiarasnd gssgsarrls frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr
      121 lrhvqlkdas tlsakglkvl kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg
      181 sfmdcsrycv vvtlsklral htlnvsetef nkhgleivve dlpllesldi sctkvdditp
      241 lkkcknrlkt lnmynlkine sanlisvlle lnelrnldis derdmhalns vlgpakpkit
      301 dflkavhcmp yltsldlsgk gdidpkdlke fikahpylrf lglvstdacf ddslinpsne
      361 dyrpglvvsg tatesqilea lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm
      421 reypqevrvq vpctaclynl tkgefaimih psilkqvvel tmiamesypt nyrlqmntll
      481 tlcsdrilqd vafdkyrcar lvlnslltfn epsmnrmsva icsllaakis tvetsllgae
      541 plymskllqm vkskmesksv ditmkftlsa lwnltdesvt tcevfldegg melclkmles
      601 fqdessvetk vlgllnniae vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll
      661 sdgprawttm itvptrdgll eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql
      721 wavwaihnvc tknpkrycsm lireggveil kylesthteq tmkpnlqslc qsilktleln
      781 s
//
LOCUS       XP_034180879             781 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180879
VERSION     XP_034180879.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="protein zyg-11 homolog B isoform X1"
                     /calculated_mol_wt=87728
     Region          87..>260
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..781
                     /gene="LOC117604496"
                     /coded_by="XM_034324988.2:230..2575"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttcnqqvnf sinsnnidil
       61 getiarasnd gssgsarrls frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr
      121 lrhvqlkdas tlsakglkvl kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg
      181 sfmdcsrycv vvtlsklral htlnvsetef nkhgleivve dlpllesldi sctkvdditp
      241 lkkcknrlkt lnmynlkine sanlisvlle lnelrnldis derdmhalns vlgpakpkit
      301 dflkavhcmp yltsldlsgk gdidpkdlke fikahpylrf lglvstdacf ddslinpsne
      361 dyrpglvvsg tatesqilea lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm
      421 reypqevrvq vpctaclynl tkgefaimih psilkqvvel tmiamesypt nyrlqmntll
      481 tlcsdrilqd vafdkyrcar lvlnslltfn epsmnrmsva icsllaakis tvetsllgae
      541 plymskllqm vkskmesksv ditmkftlsa lwnltdesvt tcevfldegg melclkmles
      601 fqdessvetk vlgllnniae vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll
      661 sdgprawttm itvptrdgll eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql
      721 wavwaihnvc tknpkrycsm lireggveil kylesthteq tmkpnlqslc qsilktleln
      781 s
//
LOCUS       XP_034180963             781 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180963
VERSION     XP_034180963.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325072.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="protein zyg-11 homolog B isoform X1"
                     /calculated_mol_wt=87728
     Region          87..>260
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..781
                     /gene="LOC117604496"
                     /coded_by="XM_034325072.2:238..2583"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttcnqqvnf sinsnnidil
       61 getiarasnd gssgsarrls frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr
      121 lrhvqlkdas tlsakglkvl kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg
      181 sfmdcsrycv vvtlsklral htlnvsetef nkhgleivve dlpllesldi sctkvdditp
      241 lkkcknrlkt lnmynlkine sanlisvlle lnelrnldis derdmhalns vlgpakpkit
      301 dflkavhcmp yltsldlsgk gdidpkdlke fikahpylrf lglvstdacf ddslinpsne
      361 dyrpglvvsg tatesqilea lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm
      421 reypqevrvq vpctaclynl tkgefaimih psilkqvvel tmiamesypt nyrlqmntll
      481 tlcsdrilqd vafdkyrcar lvlnslltfn epsmnrmsva icsllaakis tvetsllgae
      541 plymskllqm vkskmesksv ditmkftlsa lwnltdesvt tcevfldegg melclkmles
      601 fqdessvetk vlgllnniae vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll
      661 sdgprawttm itvptrdgll eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql
      721 wavwaihnvc tknpkrycsm lireggveil kylesthteq tmkpnlqslc qsilktleln
      781 s
//
LOCUS       XP_034181138             761 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181138
VERSION     XP_034181138.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325247.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..761
                     /product="protein zyg-11 homolog B isoform X2"
                     /calculated_mol_wt=85543
     Region          67..>240
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..761
                     /gene="LOC117604496"
                     /coded_by="XM_034325247.2:255..2540"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mcesprsllk icidficnnv ldlcevhlgd tgehspnlld tttctrasnd gssgsarrls
       61 frypdvflpa eiseqllsnl cekktlsdlt itlfdskstr lrhvqlkdas tlsakglkvl
      121 kqhkvvdlvv nglkitvndl isclgdwslq nlrslsvakg sfmdcsrycv vvtlsklral
      181 htlnvsetef nkhgleivve dlpllesldi sctkvdditp lkkcknrlkt lnmynlkine
      241 sanlisvlle lnelrnldis derdmhalns vlgpakpkit dflkavhcmp yltsldlsgk
      301 gdidpkdlke fikahpylrf lglvstdacf ddslinpsne dyrpglvvsg tatesqilea
      361 lrrytcrpfy lekclfnlfr lthnfleprv dviklilpgm reypqevrvq vpctaclynl
      421 tkgefaimih psilkqvvel tmiamesypt nyrlqmntll tlcsdrilqd vafdkyrcar
      481 lvlnslltfn epsmnrmsva icsllaakis tvetsllgae plymskllqm vkskmesksv
      541 ditmkftlsa lwnltdesvt tcevfldegg melclkmles fqdessvetk vlgllnniae
      601 vvhlrprlmq pkfismlsvl ltsahidvsy faagiaahll sdgprawttm itvptrdgll
      661 eqlaytvthw qtpqgemvay rsfhpffpll rcsdaypvql wavwaihnvc tknpkrycsm
      721 lireggveil kylesthteq tmkpnlqslc qsilktleln s
//
LOCUS       XP_034181224             757 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034181224
VERSION     XP_034181224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325333.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..757
                     /product="protein zyg-11 homolog B isoform X3"
                     /calculated_mol_wt=85019
     Region          63..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..757
                     /gene="LOC117604496"
                     /coded_by="XM_034325333.2:255..2528"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mlhlgdtgeh spnlldtttc nqqvnfsins nnidilgeti arasndgssg sarrlsfryp
       61 dvflpaeise qllsnlcekk tlsdltitlf dskstrlrhv qlkdastlsa kglkvlkqhk
      121 vvdlvvnglk itvndliscl gdwslqnlrs lsvakgsfmd csrycvvvtl sklralhtln
      181 vsetefnkhg leivvedlpl lesldisctk vdditplkkc knrlktlnmy nlkinesanl
      241 isvllelnel rnldisderd mhalnsvlgp akpkitdflk avhcmpylts ldlsgkgdid
      301 pkdlkefika hpylrflglv stdacfddsl inpsnedyrp glvvsgtate sqilealrry
      361 tcrpfylekc lfnlfrlthn fleprvdvik lilpgmreyp qevrvqvpct aclynltkge
      421 faimihpsil kqvveltmia mesyptnyrl qmntlltlcs drilqdvafd kyrcarlvln
      481 slltfnepsm nrmsvaicsl laakistvet sllgaeplym skllqmvksk mesksvditm
      541 kftlsalwnl tdesvttcev fldeggmelc lkmlesfqde ssvetkvlgl lnniaevvhl
      601 rprlmqpkfi smlsvlltsa hidvsyfaag iaahllsdgp rawttmitvp trdglleqla
      661 ytvthwqtpq gemvayrsfh pffpllrcsd aypvqlwavw aihnvctknp krycsmlire
      721 ggveilkyle sthteqtmkp nlqslcqsil ktlelns
//
LOCUS       XP_034181304             737 aa            linear   INV 03-SEP-2025
DEFINITION  protein zyg-11 homolog B isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034181304
VERSION     XP_034181304.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325413.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..737
                     /product="protein zyg-11 homolog B isoform X4"
                     /calculated_mol_wt=82834
     Region          43..>216
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     CDS             1..737
                     /gene="LOC117604496"
                     /coded_by="XM_034325413.2:255..2468"
                     /db_xref="GeneID:117604496"
ORIGIN      
        1 mlhlgdtgeh spnlldtttc trasndgssg sarrlsfryp dvflpaeise qllsnlcekk
       61 tlsdltitlf dskstrlrhv qlkdastlsa kglkvlkqhk vvdlvvnglk itvndliscl
      121 gdwslqnlrs lsvakgsfmd csrycvvvtl sklralhtln vsetefnkhg leivvedlpl
      181 lesldisctk vdditplkkc knrlktlnmy nlkinesanl isvllelnel rnldisderd
      241 mhalnsvlgp akpkitdflk avhcmpylts ldlsgkgdid pkdlkefika hpylrflglv
      301 stdacfddsl inpsnedyrp glvvsgtate sqilealrry tcrpfylekc lfnlfrlthn
      361 fleprvdvik lilpgmreyp qevrvqvpct aclynltkge faimihpsil kqvveltmia
      421 mesyptnyrl qmntlltlcs drilqdvafd kyrcarlvln slltfnepsm nrmsvaicsl
      481 laakistvet sllgaeplym skllqmvksk mesksvditm kftlsalwnl tdesvttcev
      541 fldeggmelc lkmlesfqde ssvetkvlgl lnniaevvhl rprlmqpkfi smlsvlltsa
      601 hidvsyfaag iaahllsdgp rawttmitvp trdglleqla ytvthwqtpq gemvayrsfh
      661 pffpllrcsd aypvqlwavw aihnvctknp krycsmlire ggveilkyle sthteqtmkp
      721 nlqslcqsil ktlelns
//
LOCUS       XP_034181434             531 aa            linear   INV 03-SEP-2025
DEFINITION  PP2C-like domain-containing protein CG9801 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181434
VERSION     XP_034181434.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325543.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..531
                     /product="PP2C-like domain-containing protein CG9801"
                     /calculated_mol_wt=58521
     Region          177..363
                     /region_name="PTC1"
                     /note="Serine/threonine protein phosphatase PrpC [Signal
                     transduction mechanisms]; COG0631"
                     /db_xref="CDD:440396"
     CDS             1..531
                     /gene="LOC117604905"
                     /coded_by="XM_034325543.2:309..1904"
                     /db_xref="GeneID:117604905"
ORIGIN      
        1 mpslrkkvag fmrqlsisnl agavenidqg eqtlrsprsl tqdfasgcfi tryldgldtk
       61 qegpailhgr npeelpirql rnfhedrgil aahtgpdngl ttvnlqqrhl sindididyi
      121 dmldqneqyr ntstittpti agvvdwfnph nmaygiattl yeknptnntn ngepiadcfg
      181 ivarsdsail aladgvnwgt kasiaarsav hgsmeylnka lfsppinnem tttkdvfial
      241 lrsfhaahsl ilqeqgmltt ltvcavlplp nsdsntgqqn ymactcnvgd slayvysrkt
      301 gvreitrgsh dihcmrdmrd algalgpvdg snpelnnltl amtevekgdi ifltsdgisd
      361 nfdpvvgkfa ilpchntvle snvkdhtegr sktrrksaen lrhtesgnsn knksnlpvve
      421 ayqrheltll rmedllrrgv sgegppcnsa khlcellldf avritaakrr iledtdlyya
      481 qnkdgqliql skqeqrsrri ktldkvsmip gkldhatvva yvvgsidsdy n
//
LOCUS       XP_034181520             531 aa            linear   INV 03-SEP-2025
DEFINITION  PP2C-like domain-containing protein CG9801 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181520
VERSION     XP_034181520.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325629.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..531
                     /product="PP2C-like domain-containing protein CG9801"
                     /calculated_mol_wt=58521
     Region          177..363
                     /region_name="PTC1"
                     /note="Serine/threonine protein phosphatase PrpC [Signal
                     transduction mechanisms]; COG0631"
                     /db_xref="CDD:440396"
     CDS             1..531
                     /gene="LOC117604905"
                     /coded_by="XM_034325629.2:242..1837"
                     /db_xref="GeneID:117604905"
ORIGIN      
        1 mpslrkkvag fmrqlsisnl agavenidqg eqtlrsprsl tqdfasgcfi tryldgldtk
       61 qegpailhgr npeelpirql rnfhedrgil aahtgpdngl ttvnlqqrhl sindididyi
      121 dmldqneqyr ntstittpti agvvdwfnph nmaygiattl yeknptnntn ngepiadcfg
      181 ivarsdsail aladgvnwgt kasiaarsav hgsmeylnka lfsppinnem tttkdvfial
      241 lrsfhaahsl ilqeqgmltt ltvcavlplp nsdsntgqqn ymactcnvgd slayvysrkt
      301 gvreitrgsh dihcmrdmrd algalgpvdg snpelnnltl amtevekgdi ifltsdgisd
      361 nfdpvvgkfa ilpchntvle snvkdhtegr sktrrksaen lrhtesgnsn knksnlpvve
      421 ayqrheltll rmedllrrgv sgegppcnsa khlcellldf avritaakrr iledtdlyya
      481 qnkdgqliql skqeqrsrri ktldkvsmip gkldhatvva yvvgsidsdy n
//
LOCUS       XP_034181608             531 aa            linear   INV 03-SEP-2025
DEFINITION  PP2C-like domain-containing protein CG9801 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181608
VERSION     XP_034181608.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325717.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..531
                     /product="PP2C-like domain-containing protein CG9801"
                     /calculated_mol_wt=58521
     Region          177..363
                     /region_name="PTC1"
                     /note="Serine/threonine protein phosphatase PrpC [Signal
                     transduction mechanisms]; COG0631"
                     /db_xref="CDD:440396"
     CDS             1..531
                     /gene="LOC117604905"
                     /coded_by="XM_034325717.2:273..1868"
                     /db_xref="GeneID:117604905"
ORIGIN      
        1 mpslrkkvag fmrqlsisnl agavenidqg eqtlrsprsl tqdfasgcfi tryldgldtk
       61 qegpailhgr npeelpirql rnfhedrgil aahtgpdngl ttvnlqqrhl sindididyi
      121 dmldqneqyr ntstittpti agvvdwfnph nmaygiattl yeknptnntn ngepiadcfg
      181 ivarsdsail aladgvnwgt kasiaarsav hgsmeylnka lfsppinnem tttkdvfial
      241 lrsfhaahsl ilqeqgmltt ltvcavlplp nsdsntgqqn ymactcnvgd slayvysrkt
      301 gvreitrgsh dihcmrdmrd algalgpvdg snpelnnltl amtevekgdi ifltsdgisd
      361 nfdpvvgkfa ilpchntvle snvkdhtegr sktrrksaen lrhtesgnsn knksnlpvve
      421 ayqrheltll rmedllrrgv sgegppcnsa khlcellldf avritaakrr iledtdlyya
      481 qnkdgqliql skqeqrsrri ktldkvsmip gkldhatvva yvvgsidsdy n
//
LOCUS       XP_034181744             228 aa            linear   INV 03-SEP-2025
DEFINITION  poly(A) binding protein nuclear 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181744
VERSION     XP_034181744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="poly(A) binding protein nuclear 1 isoform X1"
                     /calculated_mol_wt=25576
     Region          101..176
                     /region_name="RRM_II_PABPN1"
                     /note="RNA recognition motif in type II
                     polyadenylate-binding protein 2 (PABP-2) and similar
                     proteins; cd12550"
                     /db_xref="CDD:409966"
     CDS             1..228
                     /gene="Pabp2"
                     /coded_by="XM_034325853.2:156..842"
                     /db_xref="GeneID:117605031"
ORIGIN      
        1 msesdllatd hidgldglen gqdgdaimqc dgvkrelnea niddpeleai karvremeee
       61 aeklkqlqse vdkqmnmgsp pgitsplnms ledkrevdnr siyvgnvdyg ataeeleqhf
      121 hgcgsvnrvt ilcnkfdghp kgfayiefae rdsvqtamam desmfrgrqi kvmpkrtnrp
      181 glsvtnrgpr gtrgyrgvar giirgsayfg yrptrrprsy krgyympy
//
LOCUS       XP_034181837             228 aa            linear   INV 03-SEP-2025
DEFINITION  poly(A) binding protein nuclear 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181837
VERSION     XP_034181837.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325946.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="poly(A) binding protein nuclear 1 isoform X1"
                     /calculated_mol_wt=25576
     Region          101..176
                     /region_name="RRM_II_PABPN1"
                     /note="RNA recognition motif in type II
                     polyadenylate-binding protein 2 (PABP-2) and similar
                     proteins; cd12550"
                     /db_xref="CDD:409966"
     CDS             1..228
                     /gene="Pabp2"
                     /coded_by="XM_034325946.2:158..844"
                     /db_xref="GeneID:117605031"
ORIGIN      
        1 msesdllatd hidgldglen gqdgdaimqc dgvkrelnea niddpeleai karvremeee
       61 aeklkqlqse vdkqmnmgsp pgitsplnms ledkrevdnr siyvgnvdyg ataeeleqhf
      121 hgcgsvnrvt ilcnkfdghp kgfayiefae rdsvqtamam desmfrgrqi kvmpkrtnrp
      181 glsvtnrgpr gtrgyrgvar giirgsayfg yrptrrprsy krgyympy
//
LOCUS       XP_076545986             219 aa            linear   INV 03-SEP-2025
DEFINITION  poly(A) binding protein nuclear 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545986
VERSION     XP_076545986.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689871.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..219
                     /product="poly(A) binding protein nuclear 1 isoform X2"
                     /calculated_mol_wt=24379
     Region          101..176
                     /region_name="RRM_II_PABPN1"
                     /note="RNA recognition motif in type II
                     polyadenylate-binding protein 2 (PABP-2) and similar
                     proteins; cd12550"
                     /db_xref="CDD:409966"
     CDS             1..219
                     /gene="Pabp2"
                     /coded_by="XM_076689871.1:156..815"
                     /db_xref="GeneID:117605031"
ORIGIN      
        1 msesdllatd hidgldglen gqdgdaimqc dgvkrelnea niddpeleai karvremeee
       61 aeklkqlqse vdkqmnmgsp pgitsplnms ledkrevdnr siyvgnvdyg ataeeleqhf
      121 hgcgsvnrvt ilcnkfdghp kgfayiefae rdsvqtamam desmfrgrqi kvmpkrtnrp
      181 glsvtnrgpr gtrgyrgvar giirgsayfg yrptrrprl
//
LOCUS       XP_034179809             430 aa            linear   INV 03-SEP-2025
DEFINITION  Golgi reassembly-stacking protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034179809
VERSION     XP_034179809.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323918.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179809.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="Golgi reassembly-stacking protein 2"
                     /calculated_mol_wt=46182
     Region          <14..100
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Region          69..203
                     /region_name="GRASP55_65"
                     /note="GRASP55/65 PDZ-like domain; pfam04495"
                     /db_xref="CDD:427981"
     CDS             1..430
                     /gene="Grasp65"
                     /coded_by="XM_034323918.2:167..1459"
                     /db_xref="GeneID:117604133"
ORIGIN      
        1 mgsshsieip gggtegyhvl kvqegspgqk aglqaffdfi vaigntrlnq dndtlkellk
       61 ngvdkklsmt vyssktqsvr qtvivpsltw ggqgllgvsv rfcsfegsne nvwhvlevhp
      121 sspaelaglr pftdyiinad svlhesedlf tlieahesrp lklyvynted dscrdvtitp
      181 nhnwggegsl gcgigygylh ripirnvqeh ksnnsytsii ksvapsqvtt smtytggsnv
      241 vtnippgfsi ppnyissqec ttkselettv plqttytsst qtyttpqlnp atvggttttn
      301 fiphvsnqsn intthandsl sqntmpnipg mptipplptf itnttplnet tgivgipedy
      361 ttvqggispl pksnvsqshi eisllgmppi ivstslpqst sfyssvlqqn eltnvttvpt
      421 tvsptipptq
//
LOCUS       XP_034179821             420 aa            linear   INV 03-SEP-2025
DEFINITION  DNA primase large subunit isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034179821
VERSION     XP_034179821.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323930.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179821.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..420
                     /product="DNA primase large subunit isoform X1"
                     /calculated_mol_wt=49371
     Region          2..416
                     /region_name="PriL"
                     /note="Archaeal/eukaryotic core primase: Large subunit,
                     PriL; cl11970"
                     /db_xref="CDD:416393"
     Site            order(152..153,156,160,165..170)
                     /site_type="other"
                     /note="PriL-PriS interface [polypeptide binding]"
                     /db_xref="CDD:143473"
     CDS             1..420
                     /gene="LOC117604140"
                     /coded_by="XM_034323930.2:123..1385"
                     /db_xref="GeneID:117604140"
ORIGIN      
        1 mfyiqapsgq vslhvlekcv mvrleylkll yegkadefnd nfeyllensv ydkvghftlr
       61 llastsqdly dywiarkilf fktrlnhilp rqlyrllrsi lcqlrlfpnk eklinrtlid
      121 lcnlflkpsv fkhlvsksnt encrlveakv ryelvpdlir krkldlnagf avvhcskwke
      181 vilslfktyi tkelmlmktn aqctinqdir ldylyqkvhc kifqnshihg riskdnidie
      241 aknfplcmqh lhmklrnvhr lshyarwhys lflkdggmhl edainywkee yskphscssi
      301 cshnwqsnek kfiysirhly glegsrknyn spscklmsvn isspmyeggc pfkhfdintl
      361 qnllclslpn dqvtkllkti sphnpqnsca qffkllsqtn ndniiigspl qyylkminqv
//
LOCUS       XP_076545987             400 aa            linear   INV 03-SEP-2025
DEFINITION  DNA primase large subunit isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076545987
VERSION     XP_076545987.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689872.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="DNA primase large subunit isoform X2"
                     /calculated_mol_wt=47139
     Region          3..396
                     /region_name="PriL"
                     /note="Archaeal/eukaryotic core primase: Large subunit,
                     PriL; cl11970"
                     /db_xref="CDD:416393"
     Site            order(132..133,136,140,145..150)
                     /site_type="other"
                     /note="PriL-PriS interface [polypeptide binding]"
                     /db_xref="CDD:143473"
     CDS             1..400
                     /gene="LOC117604140"
                     /coded_by="XM_076689872.1:228..1430"
                     /db_xref="GeneID:117604140"
ORIGIN      
        1 mvrleylkll yegkadefnd nfeyllensv ydkvghftlr llastsqdly dywiarkilf
       61 fktrlnhilp rqlyrllrsi lcqlrlfpnk eklinrtlid lcnlflkpsv fkhlvsksnt
      121 encrlveakv ryelvpdlir krkldlnagf avvhcskwke vilslfktyi tkelmlmktn
      181 aqctinqdir ldylyqkvhc kifqnshihg riskdnidie aknfplcmqh lhmklrnvhr
      241 lshyarwhys lflkdggmhl edainywkee yskphscssi cshnwqsnek kfiysirhly
      301 glegsrknyn spscklmsvn isspmyeggc pfkhfdintl qnllclslpn dqvtkllkti
      361 sphnpqnsca qffkllsqtn ndniiigspl qyylkminqv
//
LOCUS       XP_034179836             365 aa            linear   INV 03-SEP-2025
DEFINITION  UV excision repair protein Rad23 [Osmia lignaria lignaria].
ACCESSION   XP_034179836
VERSION     XP_034179836.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323945.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179836.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="UV excision repair protein Rad23"
                     /calculated_mol_wt=39535
     Region          1..364
                     /region_name="rad23"
                     /note="UV excision repair protein Rad23; TIGR00601"
                     /db_xref="CDD:273167"
     CDS             1..365
                     /gene="Rad23"
                     /coded_by="XM_034323945.2:173..1270"
                     /db_xref="GeneID:117604145"
ORIGIN      
        1 miitlknlqq qtftveidps qtvkdlkqki etqkgfpaeh qkliyagkil tddhplaeyn
       61 idekkfivvm vtklktgngh taaeeehtst vetkeesstt ssvpqpssnp tvqgasnpas
      121 tvqeqsggst atgcaggqae sallmgedyn tmvnnimdmg yereqveqal rasfnnpdra
      181 veylvtgipa qlfedlpedq leaqeqfqeh gqhplaflrm qpqfqqmrqv iqqnpqllna
      241 vlqqigqtnp allqlisqnq eafvrmlnep vettgstggr ttpvsasnva patapgglsg
      301 apaagmgtgs dvetsiiqvt pqdkeaierl kalgfpehlv vqayfacekn enlaanflls
      361 qnldd
//
LOCUS       XP_034179905             160 aa            linear   INV 03-SEP-2025
DEFINITION  magnesium-dependent phosphatase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179905
VERSION     XP_034179905.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324014.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..160
                     /product="magnesium-dependent phosphatase 1 isoform X1"
                     /calculated_mol_wt=18561
     Region          3..160
                     /region_name="Acid_PPase"
                     /note="Acid Phosphatase; pfam12689"
                     /db_xref="CDD:372256"
     Site            order(11..15,67..68,98,125..126,129..130)
                     /site_type="active"
                     /db_xref="CDD:319804"
     CDS             1..160
                     /gene="LOC117604179"
                     /coded_by="XM_034324014.2:512..994"
                     /db_xref="GeneID:117604179"
ORIGIN      
        1 msrkpkllvf dldytlwpfw vdthvtppfr kkgndvvdah gqliryykdv peilkslsak
       61 yelgvasrts eiqgakqlln lfnwdkyfky keiypgskvt hfskihlfri qkasgidykd
      121 mmffddeqrn ivdvsklgvk cilvkndlth avvenalnnf
//
LOCUS       XP_034179914             160 aa            linear   INV 03-SEP-2025
DEFINITION  magnesium-dependent phosphatase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179914
VERSION     XP_034179914.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324023.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..160
                     /product="magnesium-dependent phosphatase 1 isoform X1"
                     /calculated_mol_wt=18561
     Region          3..160
                     /region_name="Acid_PPase"
                     /note="Acid Phosphatase; pfam12689"
                     /db_xref="CDD:372256"
     Site            order(11..15,67..68,98,125..126,129..130)
                     /site_type="active"
                     /db_xref="CDD:319804"
     CDS             1..160
                     /gene="LOC117604179"
                     /coded_by="XM_034324023.2:486..968"
                     /db_xref="GeneID:117604179"
ORIGIN      
        1 msrkpkllvf dldytlwpfw vdthvtppfr kkgndvvdah gqliryykdv peilkslsak
       61 yelgvasrts eiqgakqlln lfnwdkyfky keiypgskvt hfskihlfri qkasgidykd
      121 mmffddeqrn ivdvsklgvk cilvkndlth avvenalnnf
//
LOCUS       XP_034179923             155 aa            linear   INV 03-SEP-2025
DEFINITION  magnesium-dependent phosphatase 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179923
VERSION     XP_034179923.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324032.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="magnesium-dependent phosphatase 1 isoform X2"
                     /calculated_mol_wt=17894
     Region          3..155
                     /region_name="Acid_PPase"
                     /note="Acid Phosphatase; pfam12689"
                     /db_xref="CDD:372256"
     Site            order(11..15,67..68,98,120..121,124..125)
                     /site_type="active"
                     /db_xref="CDD:319804"
     CDS             1..155
                     /gene="LOC117604179"
                     /coded_by="XM_034324032.2:512..979"
                     /db_xref="GeneID:117604179"
ORIGIN      
        1 msrkpkllvf dldytlwpfw vdthvtppfr kkgndvvdah gqliryykdv peilkslsak
       61 yelgvasrts eiqgakqlln lfnwdkyfky keiypgskvt hfskiqkasg idykdmmffd
      121 deqrnivdvs klgvkcilvk ndlthavven alnnf
//
LOCUS       XP_034179935             151 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit NP15.6
            [Osmia lignaria lignaria].
ACCESSION   XP_034179935
VERSION     XP_034179935.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324044.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="NADH dehydrogenase [ubiquinone] 1 beta
                     subcomplex subunit NP15.6"
                     /calculated_mol_wt=17734
     Region          <44..129
                     /region_name="ESSS"
                     /note="ESSS subunit of NADH:ubiquinone oxidoreductase
                     (complex I); pfam10183"
                     /db_xref="CDD:462984"
     CDS             1..151
                     /gene="NP15.6"
                     /coded_by="XM_034324044.2:174..629"
                     /db_xref="GeneID:117604193"
ORIGIN      
        1 mknmstllrl vrsqgirnra tcltfrdnkv ynvthrnsss avkqeevdke epykvkrtwr
       61 sygfdfedek ldrhfmhqtl fvvisicfvg gsfiiaympd hklkdwaqre ayiqlryree
      121 nglplidpnv vdpskftlps eeelddmeiv i
//
LOCUS       XP_034179948              95 aa            linear   INV 03-SEP-2025
DEFINITION  dolichyl-phosphate mannosyltransferase subunit 3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179948
VERSION     XP_034179948.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324057.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..95
                     /product="dolichyl-phosphate mannosyltransferase subunit
                     3"
                     /calculated_mol_wt=10960
     Region          1..90
                     /region_name="DPM3"
                     /note="Dolichol-phosphate mannosyltransferase subunit 3
                     (DPM3); pfam08285"
                     /db_xref="CDD:462416"
     CDS             1..95
                     /gene="Dpm3"
                     /coded_by="XM_034324057.2:950..1237"
                     /db_xref="GeneID:117604201"
ORIGIN      
        1 mtklmewlsf atlffgiwia iitgnidsff itewqqvilf fplivlflcg lyaaviilyr
       61 vftfnnceda afelqeqiee akkdlqsrgv ilknk
//
LOCUS       XP_034179958              95 aa            linear   INV 03-SEP-2025
DEFINITION  dolichyl-phosphate mannosyltransferase subunit 3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179958
VERSION     XP_034179958.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324067.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..95
                     /product="dolichyl-phosphate mannosyltransferase subunit
                     3"
                     /calculated_mol_wt=10960
     Region          1..90
                     /region_name="DPM3"
                     /note="Dolichol-phosphate mannosyltransferase subunit 3
                     (DPM3); pfam08285"
                     /db_xref="CDD:462416"
     CDS             1..95
                     /gene="Dpm3"
                     /coded_by="XM_034324067.2:970..1257"
                     /db_xref="GeneID:117604201"
ORIGIN      
        1 mtklmewlsf atlffgiwia iitgnidsff itewqqvilf fplivlflcg lyaaviilyr
       61 vftfnnceda afelqeqiee akkdlqsrgv ilknk
//
LOCUS       XP_034182125            1104 aa            linear   INV 03-SEP-2025
DEFINITION  1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034182125
VERSION     XP_034182125.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326234.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1104
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase isoform X1"
                     /calculated_mol_wt=126785
     Region          17..143
                     /region_name="PH_PLC_beta"
                     /note="Phospholipase C-beta (PLC-beta) pleckstrin homology
                     (PH) domain; cd13361"
                     /db_xref="CDD:270167"
     Site            order(22,24,38,55,117)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270167"
     Site            order(25,27,35,37,39,54..55)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270167"
     Region          148..300
                     /region_name="EFh_PI-PLC"
                     /note="EF-hand motif found in eukaryotic
                     phosphoinositide-specific phospholipase C (PI-PLC, EC
                     3.1.4.11) isozymes; cl28895"
                     /db_xref="CDD:333715"
     Region          148..177
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          183..211
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          221..250
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          267..300
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          312..660
                     /region_name="PI-PLCc_beta"
                     /note="Catalytic domain of metazoan
                     phosphoinositide-specific phospholipase C-beta; cd08591"
                     /db_xref="CDD:176533"
     Site            order(328..329,358,360,375,409,461,463,586,613,615)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:176533"
     Site            order(328,375)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176533"
     Site            order(329,358,360,409)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:176533"
     Region          692..814
                     /region_name="C2_PLC_like"
                     /note="C2 domain present in Phosphoinositide-specific
                     phospholipases C (PLC); cd00275"
                     /db_xref="CDD:175974"
     Site            order(711,737,768,770,773,775)
                     /site_type="other"
                     /note="Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:175974"
     Region          <865..>1082
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          874..918
                     /region_name="DUF1154"
                     /note="Protein of unknown function (DUF1154); pfam06631"
                     /db_xref="CDD:461969"
     CDS             1..1104
                     /gene="LOC117605186"
                     /coded_by="XM_034326234.2:403..3717"
                     /db_xref="GeneID:117605186"
ORIGIN      
        1 mtkrfefnwq ievplplrqg cmfdrwseek dntdlelncm fkvdeygffi ywqsegkdgd
       61 vielcqvsdi ragglpkdpk lydklltkhg eqldekslti csgvdytnin yqhvvcpdpv
      121 takvwldgvr sithnvkann vcpltclkkh wmrlrmlvdp ngkvpvkvva rtfasgktek
      181 lvyqcladlg lpsgkndvie pddftfdafy alyhkicprn dieelfqsit qgkantinld
      241 qlvmflnekq rdptlneily plydekrale iikdyeqnet arnqktmtkd gfirylmsde
      301 napvfldrld vymdmdqpla hyyinsshnt ylsgrqfggk ssvemyrqvl lagcrcveld
      361 cwdgkgedee piithgkamc tdilfkdviy avrdtafvts dypvilsfen hcskkqqykl
      421 akycdeilgd lllkeplkey plepgvplpp psmlkrkili knkrlkpeve kqelelfrqg
      481 qfviedevke dasappvvaa vveqqsvkee vsttesvass ttvtqqqsgs saglgdtlel
      541 avvqpytgst tnvhpwlssm vnyaqpikfp gfdvaeqkni hhnmssfaet aglnclktqa
      601 iefvnynkrq msriypkgtr adssnympqv fwnagcqmvs lnfqtadlpm qlnqgkfeyn
      661 gssgyllkpd fmrradrsfd pfaespvdgv iatqcsvqvi agqflsdkkv gtyvevemyg
      721 lptdtirkef rtrvvpangl npvyneepfl frkvvlpdla alrfavydes ngkllgqriv
      781 pldglqsgyr hislrteanf pmslpmlfcn ieikiyvpdg yeglmdalta praykkpent
      841 aqnkmrglgi eesdskgnsd smsahrevak preemkfdpi tveslrqekg fqkvlrkqqk
      901 eleslkkrhq kekltvqkqh cvaiekiikg knkaelsrdp tvrravseqt vqwsrladrq
      961 rreerdlmrs hleerrntlr klcvaaqvaq qkqltarher eikemnarqa rlsvesmrev
     1021 mndktlktrg ikegrlrekq qnntkkfmee rriaqliqnr ekeklkvihe kqleelqkdm
     1081 naimdmykne emeyelgpkt efyv
//
LOCUS       XP_034182211            1102 aa            linear   INV 03-SEP-2025
DEFINITION  1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182211
VERSION     XP_034182211.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326320.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1102
                     /product="1-phosphatidylinositol 4,5-bisphosphate
                     phosphodiesterase isoform X2"
                     /calculated_mol_wt=126557
     Region          17..143
                     /region_name="PH_PLC_beta"
                     /note="Phospholipase C-beta (PLC-beta) pleckstrin homology
                     (PH) domain; cd13361"
                     /db_xref="CDD:270167"
     Site            order(22,24,38,55,117)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270167"
     Site            order(25,27,35,37,39,54..55)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270167"
     Region          148..300
                     /region_name="EFh_PI-PLC"
                     /note="EF-hand motif found in eukaryotic
                     phosphoinositide-specific phospholipase C (PI-PLC, EC
                     3.1.4.11) isozymes; cl28895"
                     /db_xref="CDD:333715"
     Region          148..177
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          183..211
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          221..250
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          267..300
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320042"
     Region          312..658
                     /region_name="PI-PLCc_beta"
                     /note="Catalytic domain of metazoan
                     phosphoinositide-specific phospholipase C-beta; cd08591"
                     /db_xref="CDD:176533"
     Site            order(328..329,358,360,375,409,461,463,584,611,613)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:176533"
     Site            order(328,375)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176533"
     Site            order(329,358,360,409)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:176533"
     Region          690..812
                     /region_name="C2_PLC_like"
                     /note="C2 domain present in Phosphoinositide-specific
                     phospholipases C (PLC); cd00275"
                     /db_xref="CDD:175974"
     Site            order(709,735,766,768,771,773)
                     /site_type="other"
                     /note="Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:175974"
     Region          <863..>1080
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          872..916
                     /region_name="DUF1154"
                     /note="Protein of unknown function (DUF1154); pfam06631"
                     /db_xref="CDD:461969"
     CDS             1..1102
                     /gene="LOC117605186"
                     /coded_by="XM_034326320.2:403..3711"
                     /db_xref="GeneID:117605186"
ORIGIN      
        1 mtkrfefnwq ievplplrqg cmfdrwseek dntdlelncm fkvdeygffi ywqsegkdgd
       61 vielcqvsdi ragglpkdpk lydklltkhg eqldekslti csgvdytnin yqhvvcpdpv
      121 takvwldgvr sithnvkann vcpltclkkh wmrlrmlvdp ngkvpvkvva rtfasgktek
      181 lvyqcladlg lpsgkndvie pddftfdafy alyhkicprn dieelfqsit qgkantinld
      241 qlvmflnekq rdptlneily plydekrale iikdyeqnet arnqktmtkd gfirylmsde
      301 napvfldrld vymdmdqpla hyyinsshnt ylsgrqfggk ssvemyrqvl lagcrcveld
      361 cwdgkgedee piithgkamc tdilfkdviy avrdtafvts dypvilsfen hcskkqqykl
      421 akycdeilgd lllkeplkey plepgvplpp psmlkrkili knkrlkpeve kqelelfrqg
      481 qfviedevke dasappvvaa vveqqseevs ttesvasstt vtqqqsgssa glgdtlelav
      541 vqpytgsttn vhpwlssmvn yaqpikfpgf dvaeqknihh nmssfaetag lnclktqaie
      601 fvnynkrqms riypkgtrad ssnympqvfw nagcqmvsln fqtadlpmql nqgkfeyngs
      661 sgyllkpdfm rradrsfdpf aespvdgvia tqcsvqviag qflsdkkvgt yvevemyglp
      721 tdtirkefrt rvvpanglnp vyneepflfr kvvlpdlaal rfavydesng kllgqrivpl
      781 dglqsgyrhi slrteanfpm slpmlfcnie ikiyvpdgye glmdaltapr aykkpentaq
      841 nkmrglgiee sdskgnsdsm sahrevakpr eemkfdpitv eslrqekgfq kvlrkqqkel
      901 eslkkrhqke kltvqkqhcv aiekiikgkn kaelsrdptv rravseqtvq wsrladrqrr
      961 eerdlmrshl eerrntlrkl cvaaqvaqqk qltarherei kemnarqarl svesmrevmn
     1021 dktlktrgik egrlrekqqn ntkkfmeerr iaqliqnrek eklkvihekq leelqkdmna
     1081 imdmykneem eyelgpktef yv
//
LOCUS       XP_034182575             205 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605377 [Osmia lignaria lignaria].
ACCESSION   XP_034182575
VERSION     XP_034182575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..205
                     /product="uncharacterized protein LOC117605377"
                     /calculated_mol_wt=23688
     CDS             1..205
                     /gene="LOC117605377"
                     /coded_by="XM_034326684.2:38..655"
                     /db_xref="GeneID:117605377"
ORIGIN      
        1 mfrllipcli wlssvrseta mnmhmpdgmm eekspmeawm kpkresyhnp cppvyshpis
       61 yspppqiylk paiqyipkpm ityvkpappp vilkptpapv ivkpvvqpkv vypiyqkpmi
      121 syapiyhkpm yyapivqkvm yespkvylkk ydlppvvhkp qisypvqyqh pkvvhvqpqy
      181 nyvkvaqpvv hpqpiyhsai kpwcp
//
LOCUS       XP_034182705             149 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605429 [Osmia lignaria lignaria].
ACCESSION   XP_034182705
VERSION     XP_034182705.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326814.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="uncharacterized protein LOC117605429"
                     /calculated_mol_wt=16822
     CDS             1..149
                     /gene="LOC117605429"
                     /coded_by="XM_034326814.2:54..503"
                     /db_xref="GeneID:117605429"
ORIGIN      
        1 mnqiilligi avfagtvsas hydedhgsst yeekskpvei piykkyaipi phpvpvevpq
       61 kieipipqpq qvpieiphpy pvevvkhvei pvekpepviv ekhvpfvvek pypvyvekkf
      121 pipvakpypv hvpiykhvfh ysskgkgwh
//
LOCUS       XP_076545988             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605494 [Osmia lignaria lignaria].
ACCESSION   XP_076545988
VERSION     XP_076545988.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689873.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117605494"
                     /calculated_mol_wt=12642
     CDS             1..116
                     /gene="LOC117605494"
                     /coded_by="XM_076689873.1:164..514"
                     /db_xref="GeneID:117605494"
ORIGIN      
        1 mvavvcegtr iprisesffp gfqgahhghg ghryqhgqld nygpvqfpgd hrapygnldi
       61 gnefyghqga igghanvadh faggpfnvag nhggfnphqa hprnyerynh ahdydy
//
LOCUS       XP_034181574             563 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine hydroxylase ple [Osmia lignaria lignaria].
ACCESSION   XP_034181574
VERSION     XP_034181574.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325683.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..563
                     /product="tyrosine hydroxylase ple"
                     /calculated_mol_wt=63911
     Region          115..559
                     /region_name="Tyr_3_monoox"
                     /note="tyrosine 3-monooxygenase, tetrameric; TIGR01269"
                     /db_xref="CDD:130336"
     CDS             1..563
                     /gene="ple"
                     /coded_by="XM_034325683.2:372..2063"
                     /db_xref="GeneID:117604993"
ORIGIN      
        1 mmavaaaqkn remfaikksy siengyparr rslvddarfe tlvvkqtkqs vleearqrtn
       61 dsitdqdhle teryasssdd eqaetlacaa khgeakaevs ssesdgrpde dynddaglte
      121 eevvlaktia ecpenenavq kaalvlrlre gisslarilk tienfkgtvt hvesrpskke
      181 glqfdvlvkv dmtrqsllql irnlrqsaal dgvtlladns vsikdpwfpr haseldncnh
      241 lmtkyepdld mnhpgfadke yrarrkiiae iafaykygda ipsipytete ndtwsrvfnt
      301 vvdlvpkyac veyqrvfkkl qeekifeahr ipqlqevsdf lkkstgftlr paaglltard
      361 flsslafrvf qstqyirhin spyhtpepdc ihellghmpl ladpsfaqfs qeiglaslga
      421 sdeeieklst iywftvefgl ckegqevkay gagllsayge llhalsdkce hrafepttta
      481 lqkyqdqeyq piyyvaesfe dakekfrrwv atmsrpfevr ydphtqrvev ldsvdrlenl
      541 vsqlntemth ltnaidkikr sfa
//
LOCUS       XP_034181588             543 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604998 [Osmia lignaria lignaria].
ACCESSION   XP_034181588
VERSION     XP_034181588.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325697.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181588.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..543
                     /product="uncharacterized protein LOC117604998"
                     /calculated_mol_wt=62141
     CDS             1..543
                     /gene="LOC117604998"
                     /coded_by="XM_034325697.2:149..1780"
                     /db_xref="GeneID:117604998"
ORIGIN      
        1 mkfllsvlia hvlqcvasrs nsdsyqedsf svfgwisylk rlslitlmis gvvlyyvpes
       61 rpharkicrv lidltadglk avlstredeq iyekikskyh rvkyeqslaa qkrvsrevqd
      121 dedfelmqkn qgkrakmfvd gdgllignre kkkinmkqck ehrhsrqrlr gsfvkdisge
      181 sstevtkpvy fysdkledls kyfknslyga krpseasven yallisgspq qmamldekyd
      241 kddvvivqdp yvkleyrecg tstdklssia easvleqegp trdyndhpte pgmesyvkdv
      301 dlnsipevds rfsvnksmyq tseyqcggec cdsgcsdilg ssadtknvlk ssrsmlsskl
      361 hkkieennen lqasnytkyn heggrfmkls nlnsrkvdfr kedkidhshq wqenghqyes
      421 tcrnvrrkis rtnnplkssc cndvsyftdr sgeaddeish lgervtvkhd ldekpqreny
      481 lykrnsddsi fdhicnefet epilknrvtp aliravnwif ggcpearkvg kegspettge
      541 wll
//
LOCUS       XP_034187175             918 aa            linear   INV 03-SEP-2025
DEFINITION  transport and golgi organization 6 [Osmia lignaria lignaria].
ACCESSION   XP_034187175
VERSION     XP_034187175.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331284.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..918
                     /product="transport and golgi organization 6"
                     /calculated_mol_wt=104525
     Region          656..772
                     /region_name="RTP1_C1"
                     /note="Required for nuclear transport of RNA pol II
                     C-terminus 1; pfam10363"
                     /db_xref="CDD:463059"
     Site            order(756..757,760,763..764,797..798,801,804..805,808,
                     839..840,843,850..851,854,885..886,889,892..893)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          780..808
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          818..855
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          863..895
                     /region_name="RTP1_C2"
                     /note="Required for nuclear transport of RNA pol II
                     C-terminus 2; pfam10304"
                     /db_xref="CDD:431206"
     Region          863..893
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..918
                     /gene="Tango6"
                     /coded_by="XM_034331284.2:249..3005"
                     /db_xref="GeneID:117607488"
ORIGIN      
        1 mdhyevlfql ttidksddeg aedfntrlqq ilqkvqpmlt qhdtkfitns iryqyvwtii
       61 hellnikasn svndeslfsv kqfkalkvsi elitamgifp gllpgvginm aklcprvtql
      121 peeklsvshk yerlqfsvrs lmelyndltf rpailaqigp llaallqvcy aplmkpktse
      181 sskqkntset efemteelyn klkndqeyft rlfnkflsec pvstsmrelm vilgvpeapa
      241 wlrfrtrqhl vqllmqpngv islvaavced vldlgehwnk ldtvsrlivv pppknsdkyy
      301 esicsqllni ltstqvkhaa aiaiccital yeynreifdk nimdvicdpl ivnansvpks
      361 esevekcies ltkcfttiet klpvrflmkv alplfclynn vrqsacvlky kikqlilhll
      421 yedslrddlf adflghgstk dfgkhlaskf gptggieitg idetlkyeef adtlldltst
      481 tkqlsnklfh yllkflaqlm lskyrkksek llrteddvve eigkqlaavk llsnladist
      541 vqetqlehph elfcfvetlf nsyikytsnm skendceilc mglmliklii nerkqgldwe
      601 mfksfvesld kfsrskipkq lasltkevie liktqdrlkq khyqdlsvdy kssdkfeeal
      661 kdltdsllpv rahglmtltk lienadscti nrreiilqlf kenlkhedsf iyltainglc
      721 tfavaypqiv ietlvqeyid mpqrisigel taetrmklge ilvkvtrglg emvsvyknll
      781 ingflcatrd pdslvrsssl sclgelckvl gfrlgdtvle vmycitciik sdkapecrra
      841 avmtstlllr gigkdtlttl gkdlvdlyrg lkylrdndqd pvlrlhahla leelncivqd
      901 flfsppklek killlnss
//
LOCUS       XP_034187308             322 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607550 [Osmia lignaria lignaria].
ACCESSION   XP_034187308
VERSION     XP_034187308.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331417.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="uncharacterized protein LOC117607550"
                     /calculated_mol_wt=36746
     CDS             1..322
                     /gene="LOC117607550"
                     /coded_by="XM_034331417.2:278..1246"
                     /db_xref="GeneID:117607550"
ORIGIN      
        1 msdqlqtsnn vqesavsnsq assscsikrk cyklffscfk npykgkskeg ttnydsnnyd
       61 yiveklvikr vpfifsgilt naseirndnf ikgiqkplik mntsetqsil ycsedlnent
      121 ipvaivnksm kvdsntvisn einnkltlst nydariieli ntltslhpdl kecekwikdp
      181 ccsnihtpks ilarnteqit pinnkefgda ksiiksknkq kiyyskqrty lnefqdndke
      241 pfiqsdqgif hslkslttff dpnqlnrens neklkqiksn sqhnqlalkn eskvlgesii
      301 nnnntldtal rwkitvkhfk sq
//
LOCUS       XP_034187439             193 aa            linear   INV 03-SEP-2025
DEFINITION  ras-like GTP-binding protein RhoL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187439
VERSION     XP_034187439.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331548.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..193
                     /product="ras-like GTP-binding protein RhoL isoform X1"
                     /calculated_mol_wt=22043
     Site            order(19,49..50,52..53,55,57,66,70..75,81,84)
                     /site_type="other"
                     /note="GEF (guanine nucleotide exchange factor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Region          23..184
                     /region_name="RHO"
                     /note="Rho (Ras homology) subfamily of Ras-like small
                     GTPases; smart00174"
                     /db_xref="CDD:197554"
     Site            24..31
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206641"
     Site            order(27..32,49,71..72,130,132,164..165)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206641"
     Site            order(48,50..51,75,81,84)
                     /site_type="other"
                     /note="GAP (GTPase-activating protein) interaction site
                     [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            order(49..50,73,81,83..84)
                     /site_type="other"
                     /note="GDI (guanine nucleotide dissociation inhibitor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            49
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206641"
     Site            50..54
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206641"
     Site            order(51,81,84)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206641"
     Site            71..74
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206641"
     Site            order(73..75,81..84,89..91)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206641"
     Site            129..132
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206641"
     Site            163..165
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206641"
     CDS             1..193
                     /gene="LOC117607608"
                     /coded_by="XM_034331548.2:365..946"
                     /db_xref="GeneID:117607608"
ORIGIN      
        1 mcnnnkenkk tlsrhrpikv ttigdgtvgk tcmlityttn efpseyvptv fdnyagtiyi
       61 dgqefdmtlw dtagqedyer irplsypntd cflicfsvns htsyenvank whpeikhhcp
      121 ntpivlvgtk gdlrnvenvd titlkeckkm kkkikaykyv ecsalkrenl eevfveaira
      181 vlkkpsnklc ctf
//
LOCUS       XP_034187522             193 aa            linear   INV 03-SEP-2025
DEFINITION  ras-like GTP-binding protein RhoL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187522
VERSION     XP_034187522.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331631.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..193
                     /product="ras-like GTP-binding protein RhoL isoform X1"
                     /calculated_mol_wt=22043
     Site            order(19,49..50,52..53,55,57,66,70..75,81,84)
                     /site_type="other"
                     /note="GEF (guanine nucleotide exchange factor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Region          23..184
                     /region_name="RHO"
                     /note="Rho (Ras homology) subfamily of Ras-like small
                     GTPases; smart00174"
                     /db_xref="CDD:197554"
     Site            24..31
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206641"
     Site            order(27..32,49,71..72,130,132,164..165)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206641"
     Site            order(48,50..51,75,81,84)
                     /site_type="other"
                     /note="GAP (GTPase-activating protein) interaction site
                     [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            order(49..50,73,81,83..84)
                     /site_type="other"
                     /note="GDI (guanine nucleotide dissociation inhibitor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            49
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206641"
     Site            50..54
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206641"
     Site            order(51,81,84)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206641"
     Site            71..74
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206641"
     Site            order(73..75,81..84,89..91)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206641"
     Site            129..132
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206641"
     Site            163..165
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206641"
     CDS             1..193
                     /gene="LOC117607608"
                     /coded_by="XM_034331631.2:441..1022"
                     /db_xref="GeneID:117607608"
ORIGIN      
        1 mcnnnkenkk tlsrhrpikv ttigdgtvgk tcmlityttn efpseyvptv fdnyagtiyi
       61 dgqefdmtlw dtagqedyer irplsypntd cflicfsvns htsyenvank whpeikhhcp
      121 ntpivlvgtk gdlrnvenvd titlkeckkm kkkikaykyv ecsalkrenl eevfveaira
      181 vlkkpsnklc ctf
//
LOCUS       XP_076545989             161 aa            linear   INV 03-SEP-2025
DEFINITION  ras-like GTP-binding protein RhoL isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545989
VERSION     XP_076545989.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689874.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="ras-like GTP-binding protein RhoL isoform X2"
                     /calculated_mol_wt=18516
     Region          1..152
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            order(16,18..19,43,49,52)
                     /site_type="other"
                     /note="GAP (GTPase-activating protein) interaction site
                     [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            order(17..18,20..21,23,25,34,38..43,49,52)
                     /site_type="other"
                     /note="GEF (guanine nucleotide exchange factor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            order(17..18,41,49,51..52)
                     /site_type="other"
                     /note="GDI (guanine nucleotide dissociation inhibitor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            17
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206641"
     Site            18..22
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206641"
     Site            order(19,49,52)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206641"
     Site            39..42
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206641"
     Site            order(41..43,49..52,57..59)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206641"
     Site            97..100
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206641"
     Site            131..133
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206641"
     CDS             1..161
                     /gene="LOC117607608"
                     /coded_by="XM_076689874.1:683..1168"
                     /db_xref="GeneID:117607608"
ORIGIN      
        1 mlityttnef pseyvptvfd nyagtiyidg qefdmtlwdt agqedyerir plsypntdcf
       61 licfsvnsht syenvankwh peikhhcpnt pivlvgtkgd lrnvenvdti tlkeckkmkk
      121 kikaykyvec salkrenlee vfveairavl kkpsnklcct f
//
LOCUS       XP_034187784             161 aa            linear   INV 03-SEP-2025
DEFINITION  ras-like GTP-binding protein RhoL isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187784
VERSION     XP_034187784.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="ras-like GTP-binding protein RhoL isoform X2"
                     /calculated_mol_wt=18516
     Region          1..152
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            order(16,18..19,43,49,52)
                     /site_type="other"
                     /note="GAP (GTPase-activating protein) interaction site
                     [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            order(17..18,20..21,23,25,34,38..43,49,52)
                     /site_type="other"
                     /note="GEF (guanine nucleotide exchange factor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            order(17..18,41,49,51..52)
                     /site_type="other"
                     /note="GDI (guanine nucleotide dissociation inhibitor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:206641"
     Site            17
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206641"
     Site            18..22
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206641"
     Site            order(19,49,52)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206641"
     Site            39..42
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206641"
     Site            order(41..43,49..52,57..59)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206641"
     Site            97..100
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206641"
     Site            131..133
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206641"
     CDS             1..161
                     /gene="LOC117607608"
                     /coded_by="XM_034331893.2:291..776"
                     /db_xref="GeneID:117607608"
ORIGIN      
        1 mlityttnef pseyvptvfd nyagtiyidg qefdmtlwdt agqedyerir plsypntdcf
       61 licfsvnsht syenvankwh peikhhcpnt pivlvgtkgd lrnvenvdti tlkeckkmkk
      121 kikaykyvec salkrenlee vfveairavl kkpsnklcct f
//
LOCUS       XP_034187907             166 aa            linear   INV 03-SEP-2025
DEFINITION  cap binding protein 20 [Osmia lignaria lignaria].
ACCESSION   XP_034187907
VERSION     XP_034187907.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332016.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="cap binding protein 20"
                     /calculated_mol_wt=18852
     Region          41..118
                     /region_name="RRM_NCBP2"
                     /note="RNA recognition motif (RRM) found in nuclear
                     cap-binding protein subunit 2 (CBP20) and similar
                     proteins; cd12240"
                     /db_xref="CDD:409686"
     Site            order(42,82,84,111,113..115)
                     /site_type="other"
                     /note="chemical substrate binding site [chemical binding]"
                     /db_xref="CDD:409686"
     Site            order(49,52..54,56..58,61..62,64,66,68,88,95,98..99,
                     101..104,106..107)
                     /site_type="other"
                     /note="subunit interface [polypeptide binding]"
                     /db_xref="CDD:409686"
     CDS             1..166
                     /gene="Cbp20"
                     /coded_by="XM_034332016.2:156..656"
                     /db_xref="GeneID:117607825"
ORIGIN      
        1 maaiktnpss spsvelssyr dqhfkgsrae qdrllrnstt lyvgnlsfyt teeqiyelfs
       61 kcgdirriim gldkykktpc gfcfveyyqr gdaencmryi ngtrlddrii rtdwdagfie
      121 grqygrgktg gqvrdeyrsd fdsgrggygk iiqqkvtpls dgafgr
//
LOCUS       XP_034185994             933 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 31 [Osmia lignaria lignaria].
ACCESSION   XP_034185994
VERSION     XP_034185994.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330103.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..933
                     /product="ubiquitin carboxyl-terminal hydrolase 31"
                     /calculated_mol_wt=105649
     Region          <166..783
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     CDS             1..933
                     /gene="LOC117606963"
                     /coded_by="XM_034330103.2:185..2986"
                     /db_xref="GeneID:117606963"
ORIGIN      
        1 mdgekasavm ksvsegelgv eevklseskt rdpasrlkrt ftlprnpfqs atrmsrrrpk
       61 qnrdskdssg ttsakernnm qqhqqqvdtm qqnmgrlhgs qsypdrpgvk kvfrrpswkk
      121 finkmvqhms nvatqshkts hrddlgssag dirvppprpa hippdrvpgv iglrnhgntc
      181 fmnavlqcls htdilaeyfv ldqykvdlsr rnklnskkyg tkgeiteqla lllkaiwscq
      241 ydpemstafk svvdkygsqy rgnlqhdaqe fllwlldkvh edlnqatkkk ykiiknsfgr
      301 pddivaaetl anhvrcnnsf vhavfqaqfr ssltcprchr qsntfdpflc vsvpvpqnhr
      361 qmnlfvnvly tsqqprqvki gvsvnqaanv relreilasd tgidenhmll teihdegfhr
      421 tfsdcqplsv itendplyci elpqlkepae qayillvwin vlakgdlrqr fgspytmqvs
      481 retsyedlqk lllkemhsvl addvltssqs pglfnirvad paatpiqdeh pcidpcvehp
      541 lyteqieqal alcaddsgpq hvklilewde atkcniiqdd tdqieehasv kqlktnselg
      601 gavtleecfd lytraeilga edawhcpycn kkqevvkklg lwslpdilvi hlkrfrqqsk
      661 qrstskltml vdfplygfdm tphlahngvq thnnvgslgg lgwspwkkpr prqqnipkyd
      721 envydlyaic nhhgqdlqgg hytafcrnpy dtqwycfddt rveavndtnl itnaaymlfy
      781 qrrgltnnsg nssaastssa gsgldhwvsk mppfyfnnkt nsnatnnnqs ksqevlcqdk
      841 iveeknmtnf nrgcrnyatl qpkkrnvate tdigqidhys ddeapcrrew aspkpvrkms
      901 sitltphvpp ppstvihsas sdpdttiihe stl
//
LOCUS       XP_034184141            2343 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606142 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184141
VERSION     XP_034184141.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328250.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2343
                     /product="uncharacterized protein LOC117606142 isoform X1"
                     /calculated_mol_wt=250598
     Region          11..>377
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          <484..861
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1991..2092
                     /region_name="GLTSCR1"
                     /note="Conserved region of unknown function on GLTSCR
                     protein; pfam15249"
                     /db_xref="CDD:464589"
     CDS             1..2343
                     /gene="LOC117606142"
                     /coded_by="XM_034328250.2:946..7977"
                     /db_xref="GeneID:117606142"
ORIGIN      
        1 mqqpqsrpll gdsvssttsp tarrsnpvav lthqqlqqlq qlqaqnssgq sltfalqqas
       61 nnpqdqgngs ttgnhivyvn qlnhlnqgti npsgtgmglp patptfgvgl nmglplslln
      121 tsltsltsnv ittsnpllnl glpsintglt tinpslnhln ainsnltpni gsssinngls
      181 glgqdlnsin tginrlntln stnissglss vntginnvnp nltslnsnin qnltslstnf
      241 tspssgvsgl tainptinan ltttsinsgi tqglnrpvna lttgltqtsl nssstqfpfq
      301 aiqsiqsiap hivgsniahv pssaisqhpn snvstisqis nsgtltsssi ftstssepih
      361 stttnvnhgs nvnlttniph lgtmhsnlsn vstsnvqhis asnepnmpln hssslsssqs
      421 tgfqpqrqys qginpglsss vtltgssqps nvastintvk smqniqsqni sspsspfsip
      481 lkspasniap ptpspspnrl llrspasnsi qsnrnspspv gtstsnnfni qmqspmqspm
      541 svsqiqspvl spyppaksph llsgnnslnn rspapggspg ppvvrpntpi lqqgmqvlqi
      601 ihgtpqgyqs tptqlvtrth lignqqiqia atkpakqppq ilpkppnqhp atgsqqkqqr
      661 vtttitnqvt qqtqpqlvla gpqpnpttat miptaqglll nqvlpstgpv ivqqqpggvq
      721 lilrtpasaq qqthtaqltq qqlvrvvtaq gmqlqgitpt ffavpngfpp aaspvirhtp
      781 sspapsansg tgnpiviqna mvqspqpqis qvgssantsn tpcsqtvveq tllpppkkka
      841 kkkkkakrkd deppkldlas imkisgigdd ddifdtdltt esdvpcqvqv esntgqnlgq
      901 lssqltipns saqnliqvpa pnttntqssv sqtfnshlvt qlqmpvqnti qgqlrfavge
      961 dgrvvlhrtp elnqsemdqa taqalirslt qgggqnspii shllgqaqtt tqstqstvlq
     1021 rqkfvkspss ssvstvtttv tpagsqsvtc atspqhvqic sksnnqqksv nvpqesnpvl
     1081 nvcvqsnlas lqtldmqask sinvqsltpi sktnqssnss lpqlnsnvlt sdvlsskpsh
     1141 atfsvqnsrl antsqassnq qnsnvstqks sqvrltsvtt nvrqnlnkis qggvhvsksl
     1201 pnsltiqgeh nitknnqnpq esitlqqetp iyqhnqqqqv taqtvqtqnv qhvfeqnlqs
     1261 sgsnaqhnsv nmlqqqsscn tmqhdtmnvl qhssiqqnti nvlqqnaagn vqnieqnlqp
     1321 gisdvaqaqq ntsmqqqnts avlhntsvqq nvnvqqqkvv aantfssvna hqfesqnavn
     1381 amqqglstqq nnqhqnivit nappsntiqq nesqkvenqs ttmlnsaann ilnnapkitp
     1441 eilnalsnln pndqlliana ngqmqvisqq llqqflagql natnqvqnqn qtqkivigep
     1501 dannqnlqgv qintptsqii vnssggapti qnniqnivvq aapsqmpqhi qiqnsnfpsq
     1561 fidnlnqqqi rnlgtsqpkk tkivkktktv vtsqnagntq vtktvtvtrp tmistntsnf
     1621 qkadnsskan tvtsqstnpv ksessqivtn gpvlppspgg hlsipsngqm vqrvqtiqlp
     1681 aqkqqllkni qsqiqailar ksgsqpdqtv ltklyqeqsk ilasgkvvst tthsvssgse
     1741 tsfnvnavst iasnlmsqns yknqtsavqt qtqtsttvla sqtlnsntpt tvqnntpsny
     1801 mnsltipqnv qgqqcvqnnq nvhqthtkqh kiyqnhgnqi lspssanmqi fsppitsitt
     1861 vqsnaqqltt vqtqqtgmqq stqcqtdpld ikpnipissp vkqelsspiq vqvqsgspag
     1921 qvasprmigk gpqriqspvn tnsnlnkqsv spgsnssplv sprqavkrpa tspicrqinr
     1981 sdlleqqlki dqngainpdv ntpflskrda ckrlvryhcl neqvlsskdl akadeifeet
     2041 akhllskfns mmnkytylll mesmrevrts elmmidrtfv aeeqsilnrl reqeakvnee
     2101 fkkeekplvp kvepglaeed klsssltnvs vkreleddfp sdemeckpvi ksasctsgdy
     2161 dewleiqkel gvypsttdsf kcknsnnsgn nsasavavtr ssktertran gecrtnvpga
     2221 svsgvqhtvi kdnceqdntp vferrwscap dlerdlleda dsldglalhs qtqcpgslvt
     2281 tesgngnehd ditaqvqsai dsilnlkkrp tntlpgsngn sasqsseskd tvldqavrsi
     2341 lgs
//
LOCUS       XP_034184226            2343 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606142 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184226
VERSION     XP_034184226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328335.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2343
                     /product="uncharacterized protein LOC117606142 isoform X1"
                     /calculated_mol_wt=250598
     Region          11..>377
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          <484..861
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1991..2092
                     /region_name="GLTSCR1"
                     /note="Conserved region of unknown function on GLTSCR
                     protein; pfam15249"
                     /db_xref="CDD:464589"
     CDS             1..2343
                     /gene="LOC117606142"
                     /coded_by="XM_034328335.2:972..8003"
                     /db_xref="GeneID:117606142"
ORIGIN      
        1 mqqpqsrpll gdsvssttsp tarrsnpvav lthqqlqqlq qlqaqnssgq sltfalqqas
       61 nnpqdqgngs ttgnhivyvn qlnhlnqgti npsgtgmglp patptfgvgl nmglplslln
      121 tsltsltsnv ittsnpllnl glpsintglt tinpslnhln ainsnltpni gsssinngls
      181 glgqdlnsin tginrlntln stnissglss vntginnvnp nltslnsnin qnltslstnf
      241 tspssgvsgl tainptinan ltttsinsgi tqglnrpvna lttgltqtsl nssstqfpfq
      301 aiqsiqsiap hivgsniahv pssaisqhpn snvstisqis nsgtltsssi ftstssepih
      361 stttnvnhgs nvnlttniph lgtmhsnlsn vstsnvqhis asnepnmpln hssslsssqs
      421 tgfqpqrqys qginpglsss vtltgssqps nvastintvk smqniqsqni sspsspfsip
      481 lkspasniap ptpspspnrl llrspasnsi qsnrnspspv gtstsnnfni qmqspmqspm
      541 svsqiqspvl spyppaksph llsgnnslnn rspapggspg ppvvrpntpi lqqgmqvlqi
      601 ihgtpqgyqs tptqlvtrth lignqqiqia atkpakqppq ilpkppnqhp atgsqqkqqr
      661 vtttitnqvt qqtqpqlvla gpqpnpttat miptaqglll nqvlpstgpv ivqqqpggvq
      721 lilrtpasaq qqthtaqltq qqlvrvvtaq gmqlqgitpt ffavpngfpp aaspvirhtp
      781 sspapsansg tgnpiviqna mvqspqpqis qvgssantsn tpcsqtvveq tllpppkkka
      841 kkkkkakrkd deppkldlas imkisgigdd ddifdtdltt esdvpcqvqv esntgqnlgq
      901 lssqltipns saqnliqvpa pnttntqssv sqtfnshlvt qlqmpvqnti qgqlrfavge
      961 dgrvvlhrtp elnqsemdqa taqalirslt qgggqnspii shllgqaqtt tqstqstvlq
     1021 rqkfvkspss ssvstvtttv tpagsqsvtc atspqhvqic sksnnqqksv nvpqesnpvl
     1081 nvcvqsnlas lqtldmqask sinvqsltpi sktnqssnss lpqlnsnvlt sdvlsskpsh
     1141 atfsvqnsrl antsqassnq qnsnvstqks sqvrltsvtt nvrqnlnkis qggvhvsksl
     1201 pnsltiqgeh nitknnqnpq esitlqqetp iyqhnqqqqv taqtvqtqnv qhvfeqnlqs
     1261 sgsnaqhnsv nmlqqqsscn tmqhdtmnvl qhssiqqnti nvlqqnaagn vqnieqnlqp
     1321 gisdvaqaqq ntsmqqqnts avlhntsvqq nvnvqqqkvv aantfssvna hqfesqnavn
     1381 amqqglstqq nnqhqnivit nappsntiqq nesqkvenqs ttmlnsaann ilnnapkitp
     1441 eilnalsnln pndqlliana ngqmqvisqq llqqflagql natnqvqnqn qtqkivigep
     1501 dannqnlqgv qintptsqii vnssggapti qnniqnivvq aapsqmpqhi qiqnsnfpsq
     1561 fidnlnqqqi rnlgtsqpkk tkivkktktv vtsqnagntq vtktvtvtrp tmistntsnf
     1621 qkadnsskan tvtsqstnpv ksessqivtn gpvlppspgg hlsipsngqm vqrvqtiqlp
     1681 aqkqqllkni qsqiqailar ksgsqpdqtv ltklyqeqsk ilasgkvvst tthsvssgse
     1741 tsfnvnavst iasnlmsqns yknqtsavqt qtqtsttvla sqtlnsntpt tvqnntpsny
     1801 mnsltipqnv qgqqcvqnnq nvhqthtkqh kiyqnhgnqi lspssanmqi fsppitsitt
     1861 vqsnaqqltt vqtqqtgmqq stqcqtdpld ikpnipissp vkqelsspiq vqvqsgspag
     1921 qvasprmigk gpqriqspvn tnsnlnkqsv spgsnssplv sprqavkrpa tspicrqinr
     1981 sdlleqqlki dqngainpdv ntpflskrda ckrlvryhcl neqvlsskdl akadeifeet
     2041 akhllskfns mmnkytylll mesmrevrts elmmidrtfv aeeqsilnrl reqeakvnee
     2101 fkkeekplvp kvepglaeed klsssltnvs vkreleddfp sdemeckpvi ksasctsgdy
     2161 dewleiqkel gvypsttdsf kcknsnnsgn nsasavavtr ssktertran gecrtnvpga
     2221 svsgvqhtvi kdnceqdntp vferrwscap dlerdlleda dsldglalhs qtqcpgslvt
     2281 tesgngnehd ditaqvqsai dsilnlkkrp tntlpgsngn sasqsseskd tvldqavrsi
     2341 lgs
//
LOCUS       XP_034184321            2343 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606142 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184321
VERSION     XP_034184321.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328430.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2343
                     /product="uncharacterized protein LOC117606142 isoform X1"
                     /calculated_mol_wt=250598
     Region          11..>377
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          <484..861
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1991..2092
                     /region_name="GLTSCR1"
                     /note="Conserved region of unknown function on GLTSCR
                     protein; pfam15249"
                     /db_xref="CDD:464589"
     CDS             1..2343
                     /gene="LOC117606142"
                     /coded_by="XM_034328430.2:1030..8061"
                     /db_xref="GeneID:117606142"
ORIGIN      
        1 mqqpqsrpll gdsvssttsp tarrsnpvav lthqqlqqlq qlqaqnssgq sltfalqqas
       61 nnpqdqgngs ttgnhivyvn qlnhlnqgti npsgtgmglp patptfgvgl nmglplslln
      121 tsltsltsnv ittsnpllnl glpsintglt tinpslnhln ainsnltpni gsssinngls
      181 glgqdlnsin tginrlntln stnissglss vntginnvnp nltslnsnin qnltslstnf
      241 tspssgvsgl tainptinan ltttsinsgi tqglnrpvna lttgltqtsl nssstqfpfq
      301 aiqsiqsiap hivgsniahv pssaisqhpn snvstisqis nsgtltsssi ftstssepih
      361 stttnvnhgs nvnlttniph lgtmhsnlsn vstsnvqhis asnepnmpln hssslsssqs
      421 tgfqpqrqys qginpglsss vtltgssqps nvastintvk smqniqsqni sspsspfsip
      481 lkspasniap ptpspspnrl llrspasnsi qsnrnspspv gtstsnnfni qmqspmqspm
      541 svsqiqspvl spyppaksph llsgnnslnn rspapggspg ppvvrpntpi lqqgmqvlqi
      601 ihgtpqgyqs tptqlvtrth lignqqiqia atkpakqppq ilpkppnqhp atgsqqkqqr
      661 vtttitnqvt qqtqpqlvla gpqpnpttat miptaqglll nqvlpstgpv ivqqqpggvq
      721 lilrtpasaq qqthtaqltq qqlvrvvtaq gmqlqgitpt ffavpngfpp aaspvirhtp
      781 sspapsansg tgnpiviqna mvqspqpqis qvgssantsn tpcsqtvveq tllpppkkka
      841 kkkkkakrkd deppkldlas imkisgigdd ddifdtdltt esdvpcqvqv esntgqnlgq
      901 lssqltipns saqnliqvpa pnttntqssv sqtfnshlvt qlqmpvqnti qgqlrfavge
      961 dgrvvlhrtp elnqsemdqa taqalirslt qgggqnspii shllgqaqtt tqstqstvlq
     1021 rqkfvkspss ssvstvtttv tpagsqsvtc atspqhvqic sksnnqqksv nvpqesnpvl
     1081 nvcvqsnlas lqtldmqask sinvqsltpi sktnqssnss lpqlnsnvlt sdvlsskpsh
     1141 atfsvqnsrl antsqassnq qnsnvstqks sqvrltsvtt nvrqnlnkis qggvhvsksl
     1201 pnsltiqgeh nitknnqnpq esitlqqetp iyqhnqqqqv taqtvqtqnv qhvfeqnlqs
     1261 sgsnaqhnsv nmlqqqsscn tmqhdtmnvl qhssiqqnti nvlqqnaagn vqnieqnlqp
     1321 gisdvaqaqq ntsmqqqnts avlhntsvqq nvnvqqqkvv aantfssvna hqfesqnavn
     1381 amqqglstqq nnqhqnivit nappsntiqq nesqkvenqs ttmlnsaann ilnnapkitp
     1441 eilnalsnln pndqlliana ngqmqvisqq llqqflagql natnqvqnqn qtqkivigep
     1501 dannqnlqgv qintptsqii vnssggapti qnniqnivvq aapsqmpqhi qiqnsnfpsq
     1561 fidnlnqqqi rnlgtsqpkk tkivkktktv vtsqnagntq vtktvtvtrp tmistntsnf
     1621 qkadnsskan tvtsqstnpv ksessqivtn gpvlppspgg hlsipsngqm vqrvqtiqlp
     1681 aqkqqllkni qsqiqailar ksgsqpdqtv ltklyqeqsk ilasgkvvst tthsvssgse
     1741 tsfnvnavst iasnlmsqns yknqtsavqt qtqtsttvla sqtlnsntpt tvqnntpsny
     1801 mnsltipqnv qgqqcvqnnq nvhqthtkqh kiyqnhgnqi lspssanmqi fsppitsitt
     1861 vqsnaqqltt vqtqqtgmqq stqcqtdpld ikpnipissp vkqelsspiq vqvqsgspag
     1921 qvasprmigk gpqriqspvn tnsnlnkqsv spgsnssplv sprqavkrpa tspicrqinr
     1981 sdlleqqlki dqngainpdv ntpflskrda ckrlvryhcl neqvlsskdl akadeifeet
     2041 akhllskfns mmnkytylll mesmrevrts elmmidrtfv aeeqsilnrl reqeakvnee
     2101 fkkeekplvp kvepglaeed klsssltnvs vkreleddfp sdemeckpvi ksasctsgdy
     2161 dewleiqkel gvypsttdsf kcknsnnsgn nsasavavtr ssktertran gecrtnvpga
     2221 svsgvqhtvi kdnceqdntp vferrwscap dlerdlleda dsldglalhs qtqcpgslvt
     2281 tesgngnehd ditaqvqsai dsilnlkkrp tntlpgsngn sasqsseskd tvldqavrsi
     2341 lgs
//
LOCUS       XP_034184491            2308 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606142 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184491
VERSION     XP_034184491.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328600.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2308
                     /product="uncharacterized protein LOC117606142 isoform X2"
                     /calculated_mol_wt=246966
     Region          11..>377
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          <484..826
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1956..2057
                     /region_name="GLTSCR1"
                     /note="Conserved region of unknown function on GLTSCR
                     protein; pfam15249"
                     /db_xref="CDD:464589"
     CDS             1..2308
                     /gene="LOC117606142"
                     /coded_by="XM_034328600.2:946..7872"
                     /db_xref="GeneID:117606142"
ORIGIN      
        1 mqqpqsrpll gdsvssttsp tarrsnpvav lthqqlqqlq qlqaqnssgq sltfalqqas
       61 nnpqdqgngs ttgnhivyvn qlnhlnqgti npsgtgmglp patptfgvgl nmglplslln
      121 tsltsltsnv ittsnpllnl glpsintglt tinpslnhln ainsnltpni gsssinngls
      181 glgqdlnsin tginrlntln stnissglss vntginnvnp nltslnsnin qnltslstnf
      241 tspssgvsgl tainptinan ltttsinsgi tqglnrpvna lttgltqtsl nssstqfpfq
      301 aiqsiqsiap hivgsniahv pssaisqhpn snvstisqis nsgtltsssi ftstssepih
      361 stttnvnhgs nvnlttniph lgtmhsnlsn vstsnvqhis asnepnmpln hssslsssqs
      421 tgfqpqrqys qginpglsss vtltgssqps nvastintvk smqniqsqni sspsspfsip
      481 lkspasniap ptpspspnrl llrspasnsi qsnrnspspv gtstsnnfni qmqspmqspm
      541 svsqiqspvl spyppaksph llsgnnslnn rspapggspg ppvvrpntpi lqqgmqvlqi
      601 ihgtpqgyqs tptqlvtrth lignqqiqia atkpakqppq ilpkppnqhp atgsqqkqqr
      661 vtttitnqvt qqtqpqlvla gpqpnpttat miptaqglll nqltqqqlvr vvtaqgmqlq
      721 gitptffavp ngfppaaspv irhtpsspap sansgtgnpi viqnamvqsp qpqisqvgss
      781 antsntpcsq tvveqtllpp pkkkakkkkk akrkddeppk ldlasimkis gigddddifd
      841 tdlttesdvp cqvqvesntg qnlgqlssql tipnssaqnl iqvpapnttn tqssvsqtfn
      901 shlvtqlqmp vqntiqgqlr favgedgrvv lhrtpelnqs emdqataqal irsltqgggq
      961 nspiishllg qaqtttqstq stvlqrqkfv kspssssvst vtttvtpags qsvtcatspq
     1021 hvqicsksnn qqksvnvpqe snpvlnvcvq snlaslqtld mqasksinvq sltpisktnq
     1081 ssnsslpqln snvltsdvls skpshatfsv qnsrlantsq assnqqnsnv stqkssqvrl
     1141 tsvttnvrqn lnkisqggvh vskslpnslt iqgehnitkn nqnpqesitl qqetpiyqhn
     1201 qqqqvtaqtv qtqnvqhvfe qnlqssgsna qhnsvnmlqq qsscntmqhd tmnvlqhssi
     1261 qqntinvlqq naagnvqnie qnlqpgisdv aqaqqntsmq qqntsavlhn tsvqqnvnvq
     1321 qqkvvaantf ssvnahqfes qnavnamqqg lstqqnnqhq nivitnapps ntiqqnesqk
     1381 venqsttmln saannilnna pkitpeilna lsnlnpndql lianangqmq visqqllqqf
     1441 lagqlnatnq vqnqnqtqki vigepdannq nlqgvqintp tsqiivnssg gaptiqnniq
     1501 nivvqaapsq mpqhiqiqns nfpsqfidnl nqqqirnlgt sqpkktkivk ktktvvtsqn
     1561 agntqvtktv tvtrptmist ntsnfqkadn sskantvtsq stnpvksess qivtngpvlp
     1621 pspgghlsip sngqmvqrvq tiqlpaqkqq llkniqsqiq ailarksgsq pdqtvltkly
     1681 qeqskilasg kvvsttthsv ssgsetsfnv navstiasnl msqnsyknqt savqtqtqts
     1741 ttvlasqtln sntpttvqnn tpsnymnslt ipqnvqgqqc vqnnqnvhqt htkqhkiyqn
     1801 hgnqilspss anmqifsppi tsittvqsna qqlttvqtqq tgmqqstqcq tdpldikpni
     1861 pisspvkqel sspiqvqvqs gspagqvasp rmigkgpqri qspvntnsnl nkqsvspgsn
     1921 ssplvsprqa vkrpatspic rqinrsdlle qqlkidqnga inpdvntpfl skrdackrlv
     1981 ryhclneqvl sskdlakade ifeetakhll skfnsmmnky tylllmesmr evrtselmmi
     2041 drtfvaeeqs ilnrlreqea kvneefkkee kplvpkvepg laeedklsss ltnvsvkrel
     2101 eddfpsdeme ckpviksasc tsgdydewle iqkelgvyps ttdsfkckns nnsgnnsasa
     2161 vavtrsskte rtrangecrt nvpgasvsgv qhtvikdnce qdntpvferr wscapdlerd
     2221 lledadsldg lalhsqtqcp gslvttesgn gnehdditaq vqsaidsiln lkkrptntlp
     2281 gsngnsasqs seskdtvldq avrsilgs
//
LOCUS       XP_034178089            1278 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603242 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178089
VERSION     XP_034178089.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178089.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1278
                     /product="uncharacterized protein LOC117603242 isoform X1"
                     /calculated_mol_wt=143769
     Region          8..127
                     /region_name="CID_RPRD_like"
                     /note="CID (CTD-Interacting Domain) of Regulation of
                     nuclear pre-mRNA domain-containing proteins; cd16981"
                     /db_xref="CDD:340778"
     Site            order(20..22,25,63,66..67,70..71,105,109)
                     /site_type="other"
                     /note="CTD binding site [polypeptide binding]"
                     /db_xref="CDD:340778"
     Region          <160..>269
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     CDS             1..1278
                     /gene="LOC117603242"
                     /coded_by="XM_034322198.2:522..4358"
                     /db_xref="GeneID:117603242"
ORIGIN      
        1 matsdfdvel ferrlhtlkd sqesiqglsa wclerrqhhk kivatwlqvl kkvkvehrlt
       61 lfylandviq yskrknfefv eswgttlqra ttmvrdekvk hrilrifkiw dqrqvydeef
      121 ladlsglisa apkkkldpqp svppeefqaa llistmrsca tleqatdarl rdlresnidi
      181 enaeelcasl kdrrrvedae kevdlavrnv enyvraleae irertqvlel leqadqfyet
      241 qrgevkivtn ayrnfgsrvk nlkkkldell ptlvspipsp dinapspspd sdielpgden
      301 qtqnqlgmid vappsmygsy sheydpvpvp apelsqgnds gdftnnfssf mggnmdfgnm
      361 rnifnersst ptgmsqpynd sieakpievi nmrpskness gvdfnissfl ktvlpssdgt
      421 qdsggipglg ldipesqves pqrstyrhsp vlgqhpvtpv isrmmgnqsa pigvpncqis
      481 hstplpvrnl saeshtpspy ssqnsqsnit vpfdgptnnn tvnplppppl pppifldden
      541 cynklppkfp twtppsepik epakweekgl gvfagknsmn ptwpgecdek pkvwmdgdpd
      601 rwnsnndstw vgpvrrknei lsetpesppi yeksgftdpv eyddsqpqes llstagdvdh
      661 rvipipmtie nqlpyrlmka advdhrnlis ltgspanhnv ndasmnvlsn annlwstgdq
      721 dyrqhmqpgd ivesvdmems ddetdskpkg rvlvdvrsqd rdmrvnnqva ssqhmdmdmr
      781 miplpptgql pgshgqmmqd vhmmhqgppp ppppppqfhp gqpfhqdqtd frrnpstdfh
      841 paqsnfhqnr pppnflqnqq dfspqefhqm hqnqpdfpqq dfeyrdnhnl rsnqeflgep
      901 qmrgrysqss ehqhrdmpfh rndrggrggr gfprnrrdry sddhkqrnmq nnrksrsqdh
      961 hrsspeilpn srplllqapd tvvildeegm piglptekei lanlehptdt eqddncqsms
     1021 qqarklshgt pnscdleqqq pvyssgsdlv heheseppsm dpdeqqpnqi nilpaltdpk
     1081 dsqrnqyvpv aeyeeqvese pvisdktcdg nesledpgqe mnfteqshht tdqldelvgt
     1141 aasndlkrts tngnfdeddn vssckkrmtm sngpqtates dsglngpipq asgdhsavyd
     1201 fdapvgpnfr prlggpvpfp pwrgglprgr gfrggprapw mdrgprgpsl gnfiprglkr
     1261 ggqfrgngfr grgrgnsw
//
LOCUS       XP_034178097            1272 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603242 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178097
VERSION     XP_034178097.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322206.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178097.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1272
                     /product="uncharacterized protein LOC117603242 isoform X2"
                     /calculated_mol_wt=143224
     Region          8..127
                     /region_name="CID_RPRD_like"
                     /note="CID (CTD-Interacting Domain) of Regulation of
                     nuclear pre-mRNA domain-containing proteins; cd16981"
                     /db_xref="CDD:340778"
     Site            order(20..22,25,63,66..67,70..71,105,109)
                     /site_type="other"
                     /note="CTD binding site [polypeptide binding]"
                     /db_xref="CDD:340778"
     Region          <160..>269
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     CDS             1..1272
                     /gene="LOC117603242"
                     /coded_by="XM_034322206.2:522..4340"
                     /db_xref="GeneID:117603242"
ORIGIN      
        1 matsdfdvel ferrlhtlkd sqesiqglsa wclerrqhhk kivatwlqvl kkvkvehrlt
       61 lfylandviq yskrknfefv eswgttlqra ttmvrdekvk hrilrifkiw dqrqvydeef
      121 ladlsglisa apkkkldpqp svppeefqaa llistmrsca tleqatdarl rdlresnidi
      181 enaeelcasl kdrrrvedae kevdlavrnv enyvraleae irertqvlel leqadqfyet
      241 qrgevkivtn ayrnfgsrvk nlkkkldell ptlvspipsp dinapspspd sdielpgden
      301 qtqnqlgmid vappsmygsy sheydpvpvp apelsqgnds gdftnnfssf mggnmdfgnm
      361 rnifnersst ptgmsqpynd sieakpievi nmrpskness gvdfnissfl ktvlpssdgt
      421 qdsggipglg ldipesqves pqrstyrhsp vlgqhpvtpv isrmmgnqsa pigvpncqis
      481 hstplpvrnl saeshtpspy ssqnsqsnit vpfdgptnnn tvnplppppl pppifldden
      541 cynklppkfp twtppsepik epakweekgk nsmnptwpge cdekpkvwmd gdpdrwnsnn
      601 dstwvgpvrr kneilsetpe sppiyeksgf tdpveyddsq pqesllstag dvdhrvipip
      661 mtienqlpyr lmkaadvdhr nlisltgspa nhnvndasmn vlsnannlws tgdqdyrqhm
      721 qpgdivesvd memsddetds kpkgrvlvdv rsqdrdmrvn nqvassqhmd mdmrmiplpp
      781 tgqlpgshgq mmqdvhmmhq gppppppppp qfhpgqpfhq dqtdfrrnps tdfhpaqsnf
      841 hqnrpppnfl qnqqdfspqe fhqmhqnqpd fpqqdfeyrd nhnlrsnqef lgepqmrgry
      901 sqssehqhrd mpfhrndrgg rggrgfprnr rdrysddhkq rnmqnnrksr sqdhhrsspe
      961 ilpnsrplll qapdtvvild eegmpiglpt ekeilanleh ptdteqddnc qsmsqqarkl
     1021 shgtpnscdl eqqqpvyssg sdlvhehese ppsmdpdeqq pnqinilpal tdpkdsqrnq
     1081 yvpvaeyeeq vesepvisdk tcdgnesled pgqemnfteq shhttdqlde lvgtaasndl
     1141 krtstngnfd eddnvssckk rmtmsngpqt atesdsglng pipqasgdhs avydfdapvg
     1201 pnfrprlggp vpfppwrggl prgrgfrggp rapwmdrgpr gpslgnfipr glkrggqfrg
     1261 ngfrgrgrgn sw
//
LOCUS       XP_034184616            1141 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-associated protein-like lingerer isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184616
VERSION     XP_034184616.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328725.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184616.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1141
                     /product="ubiquitin-associated protein-like lingerer
                     isoform X1"
                     /calculated_mol_wt=120304
     Region          66..103
                     /region_name="UBA_UBP2_like"
                     /note="UBA domain found in ubiquitin-associated protein 2
                     (UBAP-2) like proteins; cd14277"
                     /db_xref="CDD:270463"
     Region          <118..>183
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          409..441
                     /region_name="DUF3697"
                     /note="Ubiquitin-associated protein 2; pfam12478"
                     /db_xref="CDD:372135"
     Region          569..>1016
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          590..>802
                     /region_name="Chi1"
                     /note="Chitinase [Carbohydrate transport and metabolism];
                     COG3469"
                     /db_xref="CDD:442692"
     CDS             1..1141
                     /gene="lig"
                     /coded_by="XM_034328725.2:211..3636"
                     /db_xref="GeneID:117606345"
ORIGIN      
        1 msssnksvss gargtnknks qqhgksdhqt kssdsskhek aqpntvqmrh aqiidtrmgs
       61 gedpvlkeri kqvmdmtrrs edevvmalhd sdgdldravn dllegvktew evkkkkprqp
      121 sgskqnmdts ggqdesgdwd errnqrgggp prmrgranhd nrgwrgrenk enernmedgv
      181 rdgsyggnrr grggpgrpgr ggrggsrglg prtfanrndp sstssahsfn rpidtwtgne
      241 eqqqgvqepk mdawnnldst edwdneeytg sladtkvftp stsvaepppp ieepktqdlq
      301 tvqsnqnvnl sllqqeelpk lseippiqqq tliqqtqqqq svlslptmql sqqpnaisgg
      361 vltaaqtqyl tqltqqtses lkaagqttfs ssitnqpqrp tkqrprvppp skipstavem
      421 pgdavnsgig fldvqfgale fgsdsssldg sanekynsas stipgvdvts ttnavntant
      481 nnsldiettq ssttpfntts qmlsnsenlp vssehsinaq tftgrgnstg qslditkqdf
      541 tsqvspgnaa tygttttyqs qkssfqapta tpstynayst naqsaqssfq tasgtsansy
      601 satatvtqas ynsstsfpqs ntsnfsqasp sasatsgynq ptttnqvyqs atgyvpatts
      661 qyqaqsvatn tvsnsstgyq tsgyqgsssf qstpqayqqs gttftspitq tsaayqsaaq
      721 svygtygtya sqpqtashnh klnnattkds qydsntttsn sslatttapt lglsstsvns
      781 sqtkvtnsna vpkstssgvv tgssgtgnmt gggaagsmtp mlshqyimgq gvpyafqqpm
      841 ysyeelqlmq qriphmpttg yydaalgyqt tgpatslggg rgdalsgvqg vqgvqgvqga
      901 ytsisdarfa rndsnaspvp stmsqqtatq hqqpmmnptl pptyayfayg ggimpggfqy
      961 gtpaiypqia tagnagtnsg aysakpggyg sgyganasyd alasagpsae ykgagnnyts
     1021 tqtgktgtst gntntggssa tdisatmyak shvalskvns ydkqtfhsat pppfgltgsq
     1081 naglpggygt phlfiptmph qlhqplhqdg gsstgqrsnt ssqnkaqakp gyspsywtgn
     1141 n
//
LOCUS       XP_034184627            1132 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-associated protein-like lingerer isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184627
VERSION     XP_034184627.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328736.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184627.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1132
                     /product="ubiquitin-associated protein-like lingerer
                     isoform X2"
                     /calculated_mol_wt=119287
     Region          66..103
                     /region_name="UBA_UBP2_like"
                     /note="UBA domain found in ubiquitin-associated protein 2
                     (UBAP-2) like proteins; cd14277"
                     /db_xref="CDD:270463"
     Region          <118..>183
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          400..432
                     /region_name="DUF3697"
                     /note="Ubiquitin-associated protein 2; pfam12478"
                     /db_xref="CDD:372135"
     Region          560..>1007
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          581..>793
                     /region_name="Chi1"
                     /note="Chitinase [Carbohydrate transport and metabolism];
                     COG3469"
                     /db_xref="CDD:442692"
     CDS             1..1132
                     /gene="lig"
                     /coded_by="XM_034328736.2:211..3609"
                     /db_xref="GeneID:117606345"
ORIGIN      
        1 msssnksvss gargtnknks qqhgksdhqt kssdsskhek aqpntvqmrh aqiidtrmgs
       61 gedpvlkeri kqvmdmtrrs edevvmalhd sdgdldravn dllegvktew evkkkkprqp
      121 sgskqnmdts ggqdesgdwd errnqrgggp prmrgranhd nrgwrgrenk enernmedgv
      181 rdgsyggnrr grggpgrpgr ggrggsrglg prtfanrndp sstssahsfn rpidtwtgne
      241 eqqqgvqepk medwdneeyt gsladtkvft pstsvaeppp pieepktqdl qtvqsnqnvn
      301 lsllqqeelp klseippiqq qtliqqtqqq qsvlslptmq lsqqpnaisg gvltaaqtqy
      361 ltqltqqtse slkaagqttf sssitnqpqr ptkqrprvpp pskipstave mpgdavnsgi
      421 gfldvqfgal efgsdsssld gsanekynsa sstipgvdvt sttnavntan tnnsldiett
      481 qssttpfntt sqmlsnsenl pvssehsina qtftgrgnst gqslditkqd ftsqvspgna
      541 atygttttyq sqkssfqapt atpstynays tnaqsaqssf qtasgtsans ysatatvtqa
      601 synsstsfpq sntsnfsqas psasatsgyn qptttnqvyq satgyvpatt sqyqaqsvat
      661 ntvsnsstgy qtsgyqgsss fqstpqayqq sgttftspit qtsaayqsaa qsvygtygty
      721 asqpqtashn hklnnattkd sqydsnttts nsslatttap tlglsstsvn ssqtkvtnsn
      781 avpkstssgv vtgssgtgnm tgggaagsmt pmlshqyimg qgvpyafqqp mysyeelqlm
      841 qqriphmptt gyydaalgyq ttgpatslgg grgdalsgvq gvqgvqgvqg aytsisdarf
      901 arndsnaspv pstmsqqtat qhqqpmmnpt lpptyayfay gggimpggfq ygtpaiypqi
      961 atagnagtns gaysakpggy gsgyganasy dalasagpsa eykgagnnyt stqtgktgts
     1021 tgntntggss atdisatmya kshvalskvn sydkqtfhsa tpppfgltgs qnaglpggyg
     1081 tphlfiptmp hqlhqplhqd ggsstgqrsn tssqnkaqak pgyspsywtg nn
//
LOCUS       XP_034184631            1120 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-associated protein-like lingerer isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184631
VERSION     XP_034184631.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328740.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184631.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1120
                     /product="ubiquitin-associated protein-like lingerer
                     isoform X3"
                     /calculated_mol_wt=118045
     Region          66..103
                     /region_name="UBA_UBP2_like"
                     /note="UBA domain found in ubiquitin-associated protein 2
                     (UBAP-2) like proteins; cd14277"
                     /db_xref="CDD:270463"
     Region          <118..>181
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          388..420
                     /region_name="DUF3697"
                     /note="Ubiquitin-associated protein 2; pfam12478"
                     /db_xref="CDD:372135"
     Region          548..>995
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          569..>781
                     /region_name="Chi1"
                     /note="Chitinase [Carbohydrate transport and metabolism];
                     COG3469"
                     /db_xref="CDD:442692"
     CDS             1..1120
                     /gene="lig"
                     /coded_by="XM_034328740.2:211..3573"
                     /db_xref="GeneID:117606345"
ORIGIN      
        1 msssnksvss gargtnknks qqhgksdhqt kssdsskhek aqpntvqmrh aqiidtrmgs
       61 gedpvlkeri kqvmdmtrrs edevvmalhd sdgdldravn dllegvktew evkkkkprqp
      121 sgskqnmdts ggqdesgdwd errnqrgggp prmrgranhd nrgwrgrenk enernmedgv
      181 rdgsyggnrr grggpgrpgr ggrggsrglg prtfanrnee qqqgvqepkm dawnnldste
      241 dwdneeytgs ladtkvftps tsvaeppppi eepktqdlqt vqsnqnvnls llqqeelpkl
      301 seippiqqqt liqqtqqqqs vlslptmqls qqpnaisggv ltaaqtqylt qltqqtsesl
      361 kaagqttfss sitnqpqrpt kqrprvppps kipstavemp gdavnsgigf ldvqfgalef
      421 gsdsssldgs anekynsass tipgvdvtst tnavntantn nsldiettqs sttpfnttsq
      481 mlsnsenlpv ssehsinaqt ftgrgnstgq slditkqdft sqvspgnaat ygttttyqsq
      541 kssfqaptat pstynaystn aqsaqssfqt asgtsansys atatvtqasy nsstsfpqsn
      601 tsnfsqasps asatsgynqp tttnqvyqsa tgyvpattsq yqaqsvatnt vsnsstgyqt
      661 sgyqgsssfq stpqayqqsg ttftspitqt saayqsaaqs vygtygtyas qpqtashnhk
      721 lnnattkdsq ydsntttsns slatttaptl glsstsvnss qtkvtnsnav pkstssgvvt
      781 gssgtgnmtg ggaagsmtpm lshqyimgqg vpyafqqpmy syeelqlmqq riphmpttgy
      841 ydaalgyqtt gpatslgggr gdalsgvqgv qgvqgvqgay tsisdarfar ndsnaspvps
      901 tmsqqtatqh qqpmmnptlp ptyayfaygg gimpggfqyg tpaiypqiat agnagtnsga
      961 ysakpggygs gyganasyda lasagpsaey kgagnnytst qtgktgtstg ntntggssat
     1021 disatmyaks hvalskvnsy dkqtfhsatp ppfgltgsqn aglpggygtp hlfiptmphq
     1081 lhqplhqdgg sstgqrsnts sqnkaqakpg yspsywtgnn
//
LOCUS       XP_034184637            1111 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-associated protein-like lingerer isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184637
VERSION     XP_034184637.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328746.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184637.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1111
                     /product="ubiquitin-associated protein-like lingerer
                     isoform X4"
                     /calculated_mol_wt=117028
     Region          66..103
                     /region_name="UBA_UBP2_like"
                     /note="UBA domain found in ubiquitin-associated protein 2
                     (UBAP-2) like proteins; cd14277"
                     /db_xref="CDD:270463"
     Region          <118..>179
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          379..411
                     /region_name="DUF3697"
                     /note="Ubiquitin-associated protein 2; pfam12478"
                     /db_xref="CDD:372135"
     Region          539..>986
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          560..>772
                     /region_name="Chi1"
                     /note="Chitinase [Carbohydrate transport and metabolism];
                     COG3469"
                     /db_xref="CDD:442692"
     CDS             1..1111
                     /gene="lig"
                     /coded_by="XM_034328746.2:211..3546"
                     /db_xref="GeneID:117606345"
ORIGIN      
        1 msssnksvss gargtnknks qqhgksdhqt kssdsskhek aqpntvqmrh aqiidtrmgs
       61 gedpvlkeri kqvmdmtrrs edevvmalhd sdgdldravn dllegvktew evkkkkprqp
      121 sgskqnmdts ggqdesgdwd errnqrgggp prmrgranhd nrgwrgrenk enernmedgv
      181 rdgsyggnrr grggpgrpgr ggrggsrglg prtfanrnee qqqgvqepkm edwdneeytg
      241 sladtkvftp stsvaepppp ieepktqdlq tvqsnqnvnl sllqqeelpk lseippiqqq
      301 tliqqtqqqq svlslptmql sqqpnaisgg vltaaqtqyl tqltqqtses lkaagqttfs
      361 ssitnqpqrp tkqrprvppp skipstavem pgdavnsgig fldvqfgale fgsdsssldg
      421 sanekynsas stipgvdvts ttnavntant nnsldiettq ssttpfntts qmlsnsenlp
      481 vssehsinaq tftgrgnstg qslditkqdf tsqvspgnaa tygttttyqs qkssfqapta
      541 tpstynayst naqsaqssfq tasgtsansy satatvtqas ynsstsfpqs ntsnfsqasp
      601 sasatsgynq ptttnqvyqs atgyvpatts qyqaqsvatn tvsnsstgyq tsgyqgsssf
      661 qstpqayqqs gttftspitq tsaayqsaaq svygtygtya sqpqtashnh klnnattkds
      721 qydsntttsn sslatttapt lglsstsvns sqtkvtnsna vpkstssgvv tgssgtgnmt
      781 gggaagsmtp mlshqyimgq gvpyafqqpm ysyeelqlmq qriphmpttg yydaalgyqt
      841 tgpatslggg rgdalsgvqg vqgvqgvqga ytsisdarfa rndsnaspvp stmsqqtatq
      901 hqqpmmnptl pptyayfayg ggimpggfqy gtpaiypqia tagnagtnsg aysakpggyg
      961 sgyganasyd alasagpsae ykgagnnyts tqtgktgtst gntntggssa tdisatmyak
     1021 shvalskvns ydkqtfhsat pppfgltgsq naglpggygt phlfiptmph qlhqplhqdg
     1081 gsstgqrsnt ssqnkaqakp gyspsywtgn n
//
LOCUS       XP_034184639            1050 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor pathway substrate 15 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034184639
VERSION     XP_034184639.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328748.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1050
                     /product="epidermal growth factor receptor pathway
                     substrate 15 isoform X1"
                     /calculated_mol_wt=116094
     Region          18..101
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          127..221
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          267..362
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <381..>603
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1050
                     /gene="Eps-15"
                     /coded_by="XM_034328748.2:264..3416"
                     /db_xref="GeneID:117606379"
ORIGIN      
        1 maalpsptqv agshtdiyea yynqvdpngc gqigameaar flkksqlsdd vlskiwdmad
       61 pqsrglldks glfvalklca laqagrdism snlsmelppp kmgdipityt knvvnalpvi
      121 tpvstgdwsi kpserakydq lfdslqptng yipgnkvkgv lidsklpldt lgtiwdladm
      181 dkdgmldrhe fvvamhlvyk alekyaipsv lppelmppgk rkdsipvsks paalgiitta
      241 pppipplpnv ssvksmpsmd tartnvqwvv ssedqiaaek lflqadldmd gyvsgieikd
      301 vflqsglrqt vladiwglcd tcqtgklnke qfalamwlik qklrgidppa sltsdmipps
      361 mrkpsdtive nnnisgysnp eldmiskdia elvrerqtme qdiaqkeadi kikngeiksl
      421 qseldtlaat lkqlenqkge aqkrlndlka qkaevdkdls eveqkiheeq kkvdklrqqa
      481 eeqesvlraq eeelnskrqe leglkqeeqq leqqqnksrd qlneltknlq dtqlqicqak
      541 akithlqeqq rqmsdaialy dsalaagdas lvpdtslqfn peiedleydk athkedgvqe
      601 kktdsfsnan gttldgfeqd pfasskaqea ftattpdpfg safpsqntgg ftsdpfsafd
      661 nsnvikqdpf dpfgdskrtd tkavtgtatt kdpfgddpfa nlhaptrpes pspalppkka
      721 kqppprpapp rptqgptgpl raapapptps ptpdpfanan tdpfsaqqgv idnnnstsft
      781 sstgfadfan fdskcssisv wpypippqsh qflpeptlnr tapprptsra htvypklpdf
      841 tedpfrdyry edpfniadpf addaedlnan ksketgkvdp fgygttsipp rdnsfsksfd
      901 tdfsntfplt rnkidkdnak fdadfmkafs ddnrniktie sdvfsntkak tvdideafsa
      961 kseksskkhg qdfthykskt nfndkkskne tgkiwngnnt plslteeeqf vwasqqslkt
     1021 eeerrrrkqq eeaelalale lskqeksgkl
//
LOCUS       XP_034184642            1031 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor pathway substrate 15 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034184642
VERSION     XP_034184642.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328751.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1031
                     /product="epidermal growth factor receptor pathway
                     substrate 15 isoform X2"
                     /calculated_mol_wt=113926
     Region          18..101
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          127..221
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          267..362
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <381..>603
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1031
                     /gene="Eps-15"
                     /coded_by="XM_034328751.2:263..3358"
                     /db_xref="GeneID:117606379"
ORIGIN      
        1 maalpsptqv agshtdiyea yynqvdpngc gqigameaar flkksqlsdd vlskiwdmad
       61 pqsrglldks glfvalklca laqagrdism snlsmelppp kmgdipityt knvvnalpvi
      121 tpvstgdwsi kpserakydq lfdslqptng yipgnkvkgv lidsklpldt lgtiwdladm
      181 dkdgmldrhe fvvamhlvyk alekyaipsv lppelmppgk rkdsipvsks paalgiitta
      241 pppipplpnv ssvksmpsmd tartnvqwvv ssedqiaaek lflqadldmd gyvsgieikd
      301 vflqsglrqt vladiwglcd tcqtgklnke qfalamwlik qklrgidppa sltsdmipps
      361 mrkpsdtive nnnisgysnp eldmiskdia elvrerqtme qdiaqkeadi kikngeiksl
      421 qseldtlaat lkqlenqkge aqkrlndlka qkaevdkdls eveqkiheeq kkvdklrqqa
      481 eeqesvlraq eeelnskrqe leglkqeeqq leqqqnksrd qlneltknlq dtqlqicqak
      541 akithlqeqq rqmsdaialy dsalaagdas lvpdtslqfn peiedleydk athkedgvqe
      601 kktdsfsnan gttldgfeqd pfasskaqea ftattpdpfg safpsqntgg ftsdpfsafd
      661 nsnvikqdpf dpfgdskrtd tkavtgtatt kdpfgddpfa nlhaptrpes pspalppkka
      721 kqppprpapp rptqgptgpl raapapptps ptpdpfanan tdpfsaqqgv idnnnstsft
      781 sstgfadfan fdskpeptln rtapprptsr ahtvypklpd ftedpfrdyr yedpfniadp
      841 faddaedlna nksketgkvd pfgygttsip prdnsfsksf dtdfsntfpl trnkidkdna
      901 kfdadfmkaf sddnrnikti esdvfsntka ktvdideafs akseksskkh gqdfthyksk
      961 tnfndkkskn etgkiwngnn tplslteeeq fvwasqqslk teeerrrrkq qeeaelalal
     1021 elskqeksgk l
//
LOCUS       XP_034184651            1029 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor pathway substrate 15 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034184651
VERSION     XP_034184651.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1029
                     /product="epidermal growth factor receptor pathway
                     substrate 15 isoform X3"
                     /calculated_mol_wt=113601
     Region          18..101
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          127..221
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          267..362
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <379..>582
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1029
                     /gene="Eps-15"
                     /coded_by="XM_034328760.2:264..3353"
                     /db_xref="GeneID:117606379"
ORIGIN      
        1 maalpsptqv agshtdiyea yynqvdpngc gqigameaar flkksqlsdd vlskiwdmad
       61 pqsrglldks glfvalklca laqagrdism snlsmelppp kmgdipityt knvvnalpvi
      121 tpvstgdwsi kpserakydq lfdslqptng yipgnkvkgv lidsklpldt lgtiwdladm
      181 dkdgmldrhe fvvamhlvyk alekyaipsv lppelmppgk rkdsipvsks paalgiitta
      241 pppipplpnv ssvksmpsmd tartnvqwvv ssedqiaaek lflqadldmd gyvsgieikd
      301 vflqsglrqt vladiwglcd tcqtgklnke qfalamwlik qklrgidppa sltsdmipps
      361 mrkpsdtive nnnisgysnp eldmiskdia elvrerqtme qdiaqkeadi kikngeiksl
      421 qseldtlaat lkqlenqkge aqkrlndlka qvdklrqqae eqesvlraqe eelnskrqel
      481 eglkqeeqql eqqqnksrdq lneltknlqd tqlqicqaka kithlqeqqr qmsdaialyd
      541 salaagdasl vpdtslqfnp eiedleydka thkedgvqek ktdsfsnang ttldgfeqdp
      601 fasskaqeaf tattpdpfgs afpsqntggf tsdpfsafdn snvikqdpfd pfgdskrtdt
      661 kavtgtattk dpfgddpfan lhaptrpesp spalppkkak qppprpappr ptqgptgplr
      721 aapapptpsp tpdpfanant dpfsaqqgvi dnnnstsfts stgfadfanf dskcssisvw
      781 pypippqshq flpeptlnrt apprptsrah tvypklpdft edpfrdyrye dpfniadpfa
      841 ddaedlnank sketgkvdpf gygttsippr dnsfsksfdt dfsntfpltr nkidkdnakf
      901 dadfmkafsd dnrnikties dvfsntkakt vdideafsak seksskkhgq dfthyksktn
      961 fndkksknet gkiwngnntp lslteeeqfv wasqqslkte eerrrrkqqe eaelalalel
     1021 skqeksgkl
//
LOCUS       XP_034184661             813 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor pathway substrate 15 isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034184661
VERSION     XP_034184661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..813
                     /product="epidermal growth factor receptor pathway
                     substrate 15 isoform X4"
                     /calculated_mol_wt=88970
     Region          18..101
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          127..221
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          267..362
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <381..>603
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..813
                     /gene="Eps-15"
                     /coded_by="XM_034328770.2:264..2705"
                     /db_xref="GeneID:117606379"
ORIGIN      
        1 maalpsptqv agshtdiyea yynqvdpngc gqigameaar flkksqlsdd vlskiwdmad
       61 pqsrglldks glfvalklca laqagrdism snlsmelppp kmgdipityt knvvnalpvi
      121 tpvstgdwsi kpserakydq lfdslqptng yipgnkvkgv lidsklpldt lgtiwdladm
      181 dkdgmldrhe fvvamhlvyk alekyaipsv lppelmppgk rkdsipvsks paalgiitta
      241 pppipplpnv ssvksmpsmd tartnvqwvv ssedqiaaek lflqadldmd gyvsgieikd
      301 vflqsglrqt vladiwglcd tcqtgklnke qfalamwlik qklrgidppa sltsdmipps
      361 mrkpsdtive nnnisgysnp eldmiskdia elvrerqtme qdiaqkeadi kikngeiksl
      421 qseldtlaat lkqlenqkge aqkrlndlka qkaevdkdls eveqkiheeq kkvdklrqqa
      481 eeqesvlraq eeelnskrqe leglkqeeqq leqqqnksrd qlneltknlq dtqlqicqak
      541 akithlqeqq rqmsdaialy dsalaagdas lvpdtslqfn peiedleydk athkedgvqe
      601 kktdsfsnan gttldgfeqd pfasskaqea ftattpdpfg safpsqntgg ftsdpfsafd
      661 nsnvikqdpf dpfgdskrtd tkavtgtatt kdpfgddpfa nlhaptrpes pspalppkka
      721 kqppprpapp rptqgptgpl raapapptps ptpdpfanan tdpfsaqqgv idnnnstsft
      781 sstgfadfan fdskcssisv wpypippqsh qfl
//
LOCUS       XP_034184671             794 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor pathway substrate 15 isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034184671
VERSION     XP_034184671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..794
                     /product="epidermal growth factor receptor pathway
                     substrate 15 isoform X5"
                     /calculated_mol_wt=86802
     Region          18..101
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          127..221
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          267..362
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <381..>603
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..794
                     /gene="Eps-15"
                     /coded_by="XM_034328780.2:264..2648"
                     /db_xref="GeneID:117606379"
ORIGIN      
        1 maalpsptqv agshtdiyea yynqvdpngc gqigameaar flkksqlsdd vlskiwdmad
       61 pqsrglldks glfvalklca laqagrdism snlsmelppp kmgdipityt knvvnalpvi
      121 tpvstgdwsi kpserakydq lfdslqptng yipgnkvkgv lidsklpldt lgtiwdladm
      181 dkdgmldrhe fvvamhlvyk alekyaipsv lppelmppgk rkdsipvsks paalgiitta
      241 pppipplpnv ssvksmpsmd tartnvqwvv ssedqiaaek lflqadldmd gyvsgieikd
      301 vflqsglrqt vladiwglcd tcqtgklnke qfalamwlik qklrgidppa sltsdmipps
      361 mrkpsdtive nnnisgysnp eldmiskdia elvrerqtme qdiaqkeadi kikngeiksl
      421 qseldtlaat lkqlenqkge aqkrlndlka qkaevdkdls eveqkiheeq kkvdklrqqa
      481 eeqesvlraq eeelnskrqe leglkqeeqq leqqqnksrd qlneltknlq dtqlqicqak
      541 akithlqeqq rqmsdaialy dsalaagdas lvpdtslqfn peiedleydk athkedgvqe
      601 kktdsfsnan gttldgfeqd pfasskaqea ftattpdpfg safpsqntgg ftsdpfsafd
      661 nsnvikqdpf dpfgdskrtd tkavtgtatt kdpfgddpfa nlhaptrpes pspalppkka
      721 kqppprpapp rptqgptgpl raapapptps ptpdpfanan tdpfsaqqgv idnnnstsft
      781 sstgfadfan fdsk
//
LOCUS       XP_034178110            1019 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034178110
VERSION     XP_034178110.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178110.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1019
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X1"
                     /calculated_mol_wt=117802
     Region          446..563
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(467,516,518,524)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          603..724
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(619,625,671,673,679)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <882..964
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..1019
                     /gene="Mctp"
                     /coded_by="XM_034322219.2:1282..4341"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 mkkseaepsr pqrhqkpsgt pnehhkdpik epdnhpsted dvksleqecl miprsgsgsw
       61 kkisdiaaee tstdsdhstk slkltkisee shenkwkkfw ehhrdrfkhk aeeelskrsf
      121 devtaevdai frrairsmrs drseyrrgsn idldeasvdk tlseeaqcsr rdgfnarfke
      181 nklreillnd sgtieeknse eeietvrgtd eekekdtlrl ldvsesnflv eltdeekgsq
      241 eirgdpssrr wsslenlrgk mddarekssg tkieglsiek pvrikekktm gllrsslekk
      301 mgsvermled hmekiiegnv enrlwllpie swiverckhs vshgvrqdsi dvdskveekk
      361 skseeesfef kyvaaehede epkiesrleq tkksevkved pkhhdlfdkl kdnvkekmed
      421 ihryfqrtnr ladvnrrlks qiwssvvtiv lveaknllpm didglsdpyv kfrlgtekyk
      481 skvvhktlnp vwleqfdlhl yedpylgqel evtvwdrdks hqddlmgrtv idlatleret
      541 thrlwrdled gsgsiflllt isgttaseti sdlaaheetp rereqlyhry slrnsfqrlr
      601 dvghltvkvf raqglaaadl ggksdpfcvl elvnarlqtq teyktlapnw qkiftfnvkd
      661 insvlevtvy dedrdhkvef lgkvaipllk irngekrwya lkdkklrgra kgnspqille
      721 mnvvwnvvrg cvqtlnpkek kymepeikfk rqvflrnvlr lkaiivivid igkyvqscwe
      781 weskmrsiva lvffilgcyy fepymfpgaa llillryyll ygdgsglnqw isgqvsaitg
      841 tplshhtssh yhdeidegat tpgddddddd dkdkeekksl kerlqaiqev tqtvqnsigy
      901 iaslcervkn lfnftvpyls yvtmlltilg vavlyfiplr ylilvwgvnk fsrkifrphs
      961 vpnnevldli srvpddeell nyrelkplpt adcekastsg spgpsnltrr eqrkrqkaa
//
LOCUS       XP_034178117            1019 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034178117
VERSION     XP_034178117.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322226.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178117.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1019
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X1"
                     /calculated_mol_wt=117802
     Region          446..563
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(467,516,518,524)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          603..724
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(619,625,671,673,679)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <882..964
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..1019
                     /gene="Mctp"
                     /coded_by="XM_034322226.2:986..4045"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 mkkseaepsr pqrhqkpsgt pnehhkdpik epdnhpsted dvksleqecl miprsgsgsw
       61 kkisdiaaee tstdsdhstk slkltkisee shenkwkkfw ehhrdrfkhk aeeelskrsf
      121 devtaevdai frrairsmrs drseyrrgsn idldeasvdk tlseeaqcsr rdgfnarfke
      181 nklreillnd sgtieeknse eeietvrgtd eekekdtlrl ldvsesnflv eltdeekgsq
      241 eirgdpssrr wsslenlrgk mddarekssg tkieglsiek pvrikekktm gllrsslekk
      301 mgsvermled hmekiiegnv enrlwllpie swiverckhs vshgvrqdsi dvdskveekk
      361 skseeesfef kyvaaehede epkiesrleq tkksevkved pkhhdlfdkl kdnvkekmed
      421 ihryfqrtnr ladvnrrlks qiwssvvtiv lveaknllpm didglsdpyv kfrlgtekyk
      481 skvvhktlnp vwleqfdlhl yedpylgqel evtvwdrdks hqddlmgrtv idlatleret
      541 thrlwrdled gsgsiflllt isgttaseti sdlaaheetp rereqlyhry slrnsfqrlr
      601 dvghltvkvf raqglaaadl ggksdpfcvl elvnarlqtq teyktlapnw qkiftfnvkd
      661 insvlevtvy dedrdhkvef lgkvaipllk irngekrwya lkdkklrgra kgnspqille
      721 mnvvwnvvrg cvqtlnpkek kymepeikfk rqvflrnvlr lkaiivivid igkyvqscwe
      781 weskmrsiva lvffilgcyy fepymfpgaa llillryyll ygdgsglnqw isgqvsaitg
      841 tplshhtssh yhdeidegat tpgddddddd dkdkeekksl kerlqaiqev tqtvqnsigy
      901 iaslcervkn lfnftvpyls yvtmlltilg vavlyfiplr ylilvwgvnk fsrkifrphs
      961 vpnnevldli srvpddeell nyrelkplpt adcekastsg spgpsnltrr eqrkrqkaa
//
LOCUS       XP_034178126            1019 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034178126
VERSION     XP_034178126.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178126.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1019
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X1"
                     /calculated_mol_wt=117802
     Region          446..563
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(467,516,518,524)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          603..724
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(619,625,671,673,679)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <882..964
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..1019
                     /gene="Mctp"
                     /coded_by="XM_034322235.2:375..3434"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 mkkseaepsr pqrhqkpsgt pnehhkdpik epdnhpsted dvksleqecl miprsgsgsw
       61 kkisdiaaee tstdsdhstk slkltkisee shenkwkkfw ehhrdrfkhk aeeelskrsf
      121 devtaevdai frrairsmrs drseyrrgsn idldeasvdk tlseeaqcsr rdgfnarfke
      181 nklreillnd sgtieeknse eeietvrgtd eekekdtlrl ldvsesnflv eltdeekgsq
      241 eirgdpssrr wsslenlrgk mddarekssg tkieglsiek pvrikekktm gllrsslekk
      301 mgsvermled hmekiiegnv enrlwllpie swiverckhs vshgvrqdsi dvdskveekk
      361 skseeesfef kyvaaehede epkiesrleq tkksevkved pkhhdlfdkl kdnvkekmed
      421 ihryfqrtnr ladvnrrlks qiwssvvtiv lveaknllpm didglsdpyv kfrlgtekyk
      481 skvvhktlnp vwleqfdlhl yedpylgqel evtvwdrdks hqddlmgrtv idlatleret
      541 thrlwrdled gsgsiflllt isgttaseti sdlaaheetp rereqlyhry slrnsfqrlr
      601 dvghltvkvf raqglaaadl ggksdpfcvl elvnarlqtq teyktlapnw qkiftfnvkd
      661 insvlevtvy dedrdhkvef lgkvaipllk irngekrwya lkdkklrgra kgnspqille
      721 mnvvwnvvrg cvqtlnpkek kymepeikfk rqvflrnvlr lkaiivivid igkyvqscwe
      781 weskmrsiva lvffilgcyy fepymfpgaa llillryyll ygdgsglnqw isgqvsaitg
      841 tplshhtssh yhdeidegat tpgddddddd dkdkeekksl kerlqaiqev tqtvqnsigy
      901 iaslcervkn lfnftvpyls yvtmlltilg vavlyfiplr ylilvwgvnk fsrkifrphs
      961 vpnnevldli srvpddeell nyrelkplpt adcekastsg spgpsnltrr eqrkrqkaa
//
LOCUS       XP_034178134            1004 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034178134
VERSION     XP_034178134.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322243.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178134.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1004
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X2"
                     /calculated_mol_wt=116225
     Region          446..563
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(467,516,518,524)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          603..724
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(619,625,671,673,679)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <867..949
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..1004
                     /gene="Mctp"
                     /coded_by="XM_034322243.2:1282..4296"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 mkkseaepsr pqrhqkpsgt pnehhkdpik epdnhpsted dvksleqecl miprsgsgsw
       61 kkisdiaaee tstdsdhstk slkltkisee shenkwkkfw ehhrdrfkhk aeeelskrsf
      121 devtaevdai frrairsmrs drseyrrgsn idldeasvdk tlseeaqcsr rdgfnarfke
      181 nklreillnd sgtieeknse eeietvrgtd eekekdtlrl ldvsesnflv eltdeekgsq
      241 eirgdpssrr wsslenlrgk mddarekssg tkieglsiek pvrikekktm gllrsslekk
      301 mgsvermled hmekiiegnv enrlwllpie swiverckhs vshgvrqdsi dvdskveekk
      361 skseeesfef kyvaaehede epkiesrleq tkksevkved pkhhdlfdkl kdnvkekmed
      421 ihryfqrtnr ladvnrrlks qiwssvvtiv lveaknllpm didglsdpyv kfrlgtekyk
      481 skvvhktlnp vwleqfdlhl yedpylgqel evtvwdrdks hqddlmgrtv idlatleret
      541 thrlwrdled gsgsiflllt isgttaseti sdlaaheetp rereqlyhry slrnsfqrlr
      601 dvghltvkvf raqglaaadl ggksdpfcvl elvnarlqtq teyktlapnw qkiftfnvkd
      661 insvlevtvy dedrdhkvef lgkvaipllk irngekrwya lkdkklrgra kgnspqille
      721 mnvvwnvvrg cvqtlnpkek kymepeikfk rqvflrnvlr lkaiivivid igkyvqscwe
      781 weskmrsiva lvffilgcyy fepymfpgaa llillryylv saitgtplsh htsshyhdei
      841 degattpgdd ddddddkdke ekkslkerlq aiqevtqtvq nsigyiaslc ervknlfnft
      901 vpylsyvtml ltilgvavly fiplrylilv wgvnkfsrki frphsvpnne vldlisrvpd
      961 deellnyrel kplptadcek astsgspgps nltrreqrkr qkaa
//
LOCUS       XP_076545990             951 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076545990
VERSION     XP_076545990.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689875.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..951
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X3"
                     /calculated_mol_wt=108665
     Region          226..347
                     /region_name="C2A_MCTP_PRT"
                     /note="C2 domain first repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd04042"
                     /db_xref="CDD:176007"
     Site            order(241,247,294,296,302)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176007"
     Region          378..495
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(399,448,450,456)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          535..656
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(551,557,603,605,611)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <814..896
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..951
                     /gene="Mctp"
                     /coded_by="XM_076689875.1:5702..8557"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 msksvellag sedseqitvd nsrveersrl nlngsrqlsk satelrnndn sspsrrgeqg
       61 essvvhhhrh hrhatsvaqr thtffatlks rwtrsrsker kkskdagnll sqdvpyrsgg
      121 vesdyaadys sehsksssat qsparhclnh pesplvrggr snvvtraeds pgkcsdtrsk
      181 gslsfqasrd sheeargsis qddsafilee tarrrelalr qhaffqlrlh irrgaslvam
      241 drcgasdpyv kvksagrllh ksrtvhrdln pvwdesvtlp iedpfqpltf kvfdydwglq
      301 ddfmgeaqld ltqielgqpq dvvlelkdha rpkqhlgeiy lsvtlwprnq qekeqyfqrt
      361 nrladvnrrl ksqiwssvvt ivlveaknll pmdidglsdp yvkfrlgtek ykskvvhktl
      421 npvwleqfdl hlyedpylgq elevtvwdrd kshqddlmgr tvidlatler etthrlwrdl
      481 edgsgsifll ltisgttase tisdlaahee tprereqlyh ryslrnsfqr lrdvghltvk
      541 vfraqglaaa dlggksdpfc vlelvnarlq tqteyktlap nwqkiftfnv kdinsvlevt
      601 vydedrdhkv eflgkvaipl lkirngekrw yalkdkklrg rakgnspqil lemnvvwnvv
      661 rgcvqtlnpk ekkymepeik fkrqvflrnv lrlkaiiviv idigkyvqsc weweskmrsi
      721 valvffilgc yyfepymfpg aallillryy llygdgsgln qwisgqvsai tgtplshhts
      781 shyhdeideg attpgddddd dddkdkeekk slkerlqaiq evtqtvqnsi gyiaslcerv
      841 knlfnftvpy lsyvtmllti lgvavlyfip lrylilvwgv nkfsrkifrp hsvpnnevld
      901 lisrvpddee llnyrelkpl ptadcekast sgspgpsnlt rreqrkrqka a
//
LOCUS       XP_076545991             951 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076545991
VERSION     XP_076545991.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689876.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..951
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X3"
                     /calculated_mol_wt=108665
     Region          226..347
                     /region_name="C2A_MCTP_PRT"
                     /note="C2 domain first repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd04042"
                     /db_xref="CDD:176007"
     Site            order(241,247,294,296,302)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176007"
     Region          378..495
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(399,448,450,456)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          535..656
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(551,557,603,605,611)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <814..896
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..951
                     /gene="Mctp"
                     /coded_by="XM_076689876.1:6129..8984"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 msksvellag sedseqitvd nsrveersrl nlngsrqlsk satelrnndn sspsrrgeqg
       61 essvvhhhrh hrhatsvaqr thtffatlks rwtrsrsker kkskdagnll sqdvpyrsgg
      121 vesdyaadys sehsksssat qsparhclnh pesplvrggr snvvtraeds pgkcsdtrsk
      181 gslsfqasrd sheeargsis qddsafilee tarrrelalr qhaffqlrlh irrgaslvam
      241 drcgasdpyv kvksagrllh ksrtvhrdln pvwdesvtlp iedpfqpltf kvfdydwglq
      301 ddfmgeaqld ltqielgqpq dvvlelkdha rpkqhlgeiy lsvtlwprnq qekeqyfqrt
      361 nrladvnrrl ksqiwssvvt ivlveaknll pmdidglsdp yvkfrlgtek ykskvvhktl
      421 npvwleqfdl hlyedpylgq elevtvwdrd kshqddlmgr tvidlatler etthrlwrdl
      481 edgsgsifll ltisgttase tisdlaahee tprereqlyh ryslrnsfqr lrdvghltvk
      541 vfraqglaaa dlggksdpfc vlelvnarlq tqteyktlap nwqkiftfnv kdinsvlevt
      601 vydedrdhkv eflgkvaipl lkirngekrw yalkdkklrg rakgnspqil lemnvvwnvv
      661 rgcvqtlnpk ekkymepeik fkrqvflrnv lrlkaiiviv idigkyvqsc weweskmrsi
      721 valvffilgc yyfepymfpg aallillryy llygdgsgln qwisgqvsai tgtplshhts
      781 shyhdeideg attpgddddd dddkdkeekk slkerlqaiq evtqtvqnsi gyiaslcerv
      841 knlfnftvpy lsyvtmllti lgvavlyfip lrylilvwgv nkfsrkifrp hsvpnnevld
      901 lisrvpddee llnyrelkpl ptadcekast sgspgpsnlt rreqrkrqka a
//
LOCUS       XP_076545992             951 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076545992
VERSION     XP_076545992.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689877.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..951
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X3"
                     /calculated_mol_wt=108665
     Region          226..347
                     /region_name="C2A_MCTP_PRT"
                     /note="C2 domain first repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd04042"
                     /db_xref="CDD:176007"
     Site            order(241,247,294,296,302)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176007"
     Region          378..495
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(399,448,450,456)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          535..656
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(551,557,603,605,611)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <814..896
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..951
                     /gene="Mctp"
                     /coded_by="XM_076689877.1:1447..4302"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 msksvellag sedseqitvd nsrveersrl nlngsrqlsk satelrnndn sspsrrgeqg
       61 essvvhhhrh hrhatsvaqr thtffatlks rwtrsrsker kkskdagnll sqdvpyrsgg
      121 vesdyaadys sehsksssat qsparhclnh pesplvrggr snvvtraeds pgkcsdtrsk
      181 gslsfqasrd sheeargsis qddsafilee tarrrelalr qhaffqlrlh irrgaslvam
      241 drcgasdpyv kvksagrllh ksrtvhrdln pvwdesvtlp iedpfqpltf kvfdydwglq
      301 ddfmgeaqld ltqielgqpq dvvlelkdha rpkqhlgeiy lsvtlwprnq qekeqyfqrt
      361 nrladvnrrl ksqiwssvvt ivlveaknll pmdidglsdp yvkfrlgtek ykskvvhktl
      421 npvwleqfdl hlyedpylgq elevtvwdrd kshqddlmgr tvidlatler etthrlwrdl
      481 edgsgsifll ltisgttase tisdlaahee tprereqlyh ryslrnsfqr lrdvghltvk
      541 vfraqglaaa dlggksdpfc vlelvnarlq tqteyktlap nwqkiftfnv kdinsvlevt
      601 vydedrdhkv eflgkvaipl lkirngekrw yalkdkklrg rakgnspqil lemnvvwnvv
      661 rgcvqtlnpk ekkymepeik fkrqvflrnv lrlkaiiviv idigkyvqsc weweskmrsi
      721 valvffilgc yyfepymfpg aallillryy llygdgsgln qwisgqvsai tgtplshhts
      781 shyhdeideg attpgddddd dddkdkeekk slkerlqaiq evtqtvqnsi gyiaslcerv
      841 knlfnftvpy lsyvtmllti lgvavlyfip lrylilvwgv nkfsrkifrp hsvpnnevld
      901 lisrvpddee llnyrelkpl ptadcekast sgspgpsnlt rreqrkrqka a
//
LOCUS       XP_076545993             936 aa            linear   INV 03-SEP-2025
DEFINITION  multiple C2 domain and transmembrane region protein isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_076545993
VERSION     XP_076545993.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689878.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..936
                     /product="multiple C2 domain and transmembrane region
                     protein isoform X4"
                     /calculated_mol_wt=107089
     Region          226..347
                     /region_name="C2A_MCTP_PRT"
                     /note="C2 domain first repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd04042"
                     /db_xref="CDD:176007"
     Site            order(241,247,294,296,302)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176007"
     Region          378..495
                     /region_name="C2B_MCTP_PRT"
                     /note="C2 domain second repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08376"
                     /db_xref="CDD:176022"
     Site            order(399,448,450,456)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176022"
     Region          535..656
                     /region_name="C2C_MCTP_PRT"
                     /note="C2 domain third repeat found in Multiple C2 domain
                     and Transmembrane region Proteins (MCTP); cd08377"
                     /db_xref="CDD:176023"
     Site            order(551,557,603,605,611)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176023"
     Region          <799..881
                     /region_name="PRT_C"
                     /note="Plant phosphoribosyltransferase C-terminal;
                     pfam08372"
                     /db_xref="CDD:337028"
     CDS             1..936
                     /gene="Mctp"
                     /coded_by="XM_076689878.1:6149..8959"
                     /db_xref="GeneID:117603251"
ORIGIN      
        1 msksvellag sedseqitvd nsrveersrl nlngsrqlsk satelrnndn sspsrrgeqg
       61 essvvhhhrh hrhatsvaqr thtffatlks rwtrsrsker kkskdagnll sqdvpyrsgg
      121 vesdyaadys sehsksssat qsparhclnh pesplvrggr snvvtraeds pgkcsdtrsk
      181 gslsfqasrd sheeargsis qddsafilee tarrrelalr qhaffqlrlh irrgaslvam
      241 drcgasdpyv kvksagrllh ksrtvhrdln pvwdesvtlp iedpfqpltf kvfdydwglq
      301 ddfmgeaqld ltqielgqpq dvvlelkdha rpkqhlgeiy lsvtlwprnq qekeqyfqrt
      361 nrladvnrrl ksqiwssvvt ivlveaknll pmdidglsdp yvkfrlgtek ykskvvhktl
      421 npvwleqfdl hlyedpylgq elevtvwdrd kshqddlmgr tvidlatler etthrlwrdl
      481 edgsgsifll ltisgttase tisdlaahee tprereqlyh ryslrnsfqr lrdvghltvk
      541 vfraqglaaa dlggksdpfc vlelvnarlq tqteyktlap nwqkiftfnv kdinsvlevt
      601 vydedrdhkv eflgkvaipl lkirngekrw yalkdkklrg rakgnspqil lemnvvwnvv
      661 rgcvqtlnpk ekkymepeik fkrqvflrnv lrlkaiiviv idigkyvqsc weweskmrsi
      721 valvffilgc yyfepymfpg aallillryy lvsaitgtpl shhtsshyhd eidegattpg
      781 ddddddddkd keekkslker lqaiqevtqt vqnsigyias lcervknlfn ftvpylsyvt
      841 mlltilgvav lyfiplryli lvwgvnkfsr kifrphsvpn nevldlisrv pddeellnyr
      901 elkplptadc ekastsgspg psnltrreqr krqkaa
//
LOCUS       XP_034184682             538 aa            linear   INV 03-SEP-2025
DEFINITION  sterol carrier protein X-related thiolase [Osmia lignaria
            lignaria].
ACCESSION   XP_034184682
VERSION     XP_034184682.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328791.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184682.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..538
                     /product="sterol carrier protein X-related thiolase"
                     /calculated_mol_wt=58493
     Region          1..392
                     /region_name="PRK08256"
                     /note="lipid-transfer protein; Provisional"
                     /db_xref="CDD:181327"
     Region          434..525
                     /region_name="SCP2"
                     /note="SCP-2 sterol transfer family; pfam02036"
                     /db_xref="CDD:460423"
     CDS             1..538
                     /gene="ScpX"
                     /coded_by="XM_034328791.2:217..1833"
                     /db_xref="GeneID:117606406"
ORIGIN      
        1 mkvyvvgvgm tkfekpgkrd dfdypdmake avtkalqdar ipyesvkaac vgyvygdstc
       61 gqralyqvgl tgcpiynvnn ncstgstall mgkqiiengs vdcvialgfe kmergslmsk
      121 yldrtnpmdk hvelmseiag mddspvtaql fgnagiehmk kygtkpehfa kvayknhlhs
      181 innpysqfqe kytleqimks pkvfgpltkl qccptsdgsa avvlanedfv hkhklesqav
      241 eivgmemvtd tpstfseksc ikligydmtk iaaeklftqt pykpsdvnvv elhdcfstne
      301 lityealglc ppgqggklvd agdntyggky vinpsgglis kghplgatgl aqctelcwql
      361 rglagkrqvp nvklalqhni glggavvval yrmgfpdsaq satkkavngt pdpnsfkana
      421 lfkvlevamq ddqdnliekv rgvygikitk gangeegfwi vdaktgkgkv eyfgttkpdv
      481 iiaindndiv dfisgklnpq kaffqgkiki qgnmgmamkl idlqkraakk iellrskl
//
LOCUS       XP_034184695             472 aa            linear   INV 03-SEP-2025
DEFINITION  Ig-like V-type domain-containing protein FAM187A [Osmia lignaria
            lignaria].
ACCESSION   XP_034184695
VERSION     XP_034184695.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328804.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184695.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..472
                     /product="Ig-like V-type domain-containing protein
                     FAM187A"
                     /calculated_mol_wt=54678
     Region          69..149
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..472
                     /gene="LOC117606414"
                     /coded_by="XM_034328804.2:92..1510"
                     /db_xref="GeneID:117606414"
ORIGIN      
        1 mnllktysel rkkinkilnk pkenellldi nefeyprane tyreihkqlw qeyynclrka
       61 ytniesgktm hpealiameg tlvtlqcqic ispietyann niewyfnnnd snetstiiee
      121 ldnilispne keliihniks eqagqywckl gdmlstsyyv sidtnseeik ivypntapnk
      181 phaipkktia eynlnvyttw tkwspcskcn mvgkkvrygy ctissnvvtk etkrqitkde
      241 qnempeditq nevlnegvnd kirtilrlfr nklpckskhv pkelldisdi ndrktemmvr
      301 yckikcsqnt ifevrdkqgn viesannsag iysmiqgipv psppvirtti yqkydkkail
      361 icpgslntni pitwnvnnkf lnptviqdqs qgriyinpqm hiifkslkfe dtnvyscwqk
      421 dkiigvikln vtgeielqtn ysvimvggil iiivfmmvfw rafqgrkrft vh
//
LOCUS       XP_034184718             393 aa            linear   INV 03-SEP-2025
DEFINITION  PCI domain-containing protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184718
VERSION     XP_034184718.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328827.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="PCI domain-containing protein 2 isoform X1"
                     /calculated_mol_wt=45417
     Region          <132..387
                     /region_name="PINT"
                     /note="motif in proteasome subunits, Int-6, Nip-1 and
                     TRIP-15; cl02111"
                     /db_xref="CDD:470471"
     CDS             1..393
                     /gene="PCID2"
                     /coded_by="XM_034328827.2:226..1407"
                     /db_xref="GeneID:117606418"
ORIGIN      
        1 mdsylmqvrr mwlnqdgdpl admlslrhgh isipqigset amtktmehis aplddlvlyh
       61 lktiaamnkd drlamynyqs savqsltkil qmqkeenwml pvmnvmclel rllavcvenm
      121 ktnknmkpge ilekcadclm gcfrvcacdn rsseddtkrw gmlalvnqll kiyfrinklh
      181 lcrpliraie sspykahfal aqqitykffv grkamfdsdy kaadeyltya fehchkqstk
      241 nkrliltylv pvkmllgymp khsllekynl mefgelmeav kkgdlcnlek vmekhetffi
      301 gagiylivek lkiiayrnlf kkvylvlnih qipiqdllsa lqmhelndmd mdevecivan
      361 liyegkikgy ishqhkklvi skqnpfppls tip
//
LOCUS       XP_034184709             393 aa            linear   INV 03-SEP-2025
DEFINITION  PCI domain-containing protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184709
VERSION     XP_034184709.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328818.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="PCI domain-containing protein 2 isoform X1"
                     /calculated_mol_wt=45417
     Region          <132..387
                     /region_name="PINT"
                     /note="motif in proteasome subunits, Int-6, Nip-1 and
                     TRIP-15; cl02111"
                     /db_xref="CDD:470471"
     CDS             1..393
                     /gene="PCID2"
                     /coded_by="XM_034328818.2:270..1451"
                     /db_xref="GeneID:117606418"
ORIGIN      
        1 mdsylmqvrr mwlnqdgdpl admlslrhgh isipqigset amtktmehis aplddlvlyh
       61 lktiaamnkd drlamynyqs savqsltkil qmqkeenwml pvmnvmclel rllavcvenm
      121 ktnknmkpge ilekcadclm gcfrvcacdn rsseddtkrw gmlalvnqll kiyfrinklh
      181 lcrpliraie sspykahfal aqqitykffv grkamfdsdy kaadeyltya fehchkqstk
      241 nkrliltylv pvkmllgymp khsllekynl mefgelmeav kkgdlcnlek vmekhetffi
      301 gagiylivek lkiiayrnlf kkvylvlnih qipiqdllsa lqmhelndmd mdevecivan
      361 liyegkikgy ishqhkklvi skqnpfppls tip
//
LOCUS       XP_034184727             327 aa            linear   INV 03-SEP-2025
DEFINITION  PCI domain-containing protein 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184727
VERSION     XP_034184727.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328836.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="PCI domain-containing protein 2 isoform X2"
                     /calculated_mol_wt=37956
     Region          <66..321
                     /region_name="PINT"
                     /note="motif in proteasome subunits, Int-6, Nip-1 and
                     TRIP-15; cl02111"
                     /db_xref="CDD:470471"
     CDS             1..327
                     /gene="PCID2"
                     /coded_by="XM_034328836.2:135..1118"
                     /db_xref="GeneID:117606418"
ORIGIN      
        1 mnkddrlamy nyqssavqsl tkilqmqkee nwmlpvmnvm clelrllavc venmktnknm
       61 kpgeilekca dclmgcfrvc acdnrssedd tkrwgmlalv nqllkiyfri nklhlcrpli
      121 raiesspyka hfalaqqity kffvgrkamf dsdykaadey ltyafehchk qstknkrlil
      181 tylvpvkmll gympkhslle kynlmefgel meavkkgdlc nlekvmekhe tffigagiyl
      241 iveklkiiay rnlfkkvylv lnihqipiqd llsalqmhel ndmdmdevec ivanliyegk
      301 ikgyishqhk klviskqnpf pplstip
//
LOCUS       XP_076545994            2253 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603273 [Osmia lignaria lignaria].
ACCESSION   XP_076545994
VERSION     XP_076545994.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689879.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2253
                     /product="uncharacterized protein LOC117603273"
                     /calculated_mol_wt=254743
     Region          <273..>525
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..2253
                     /gene="LOC117603273"
                     /coded_by="XM_076689879.1:5471..12232"
                     /db_xref="GeneID:117603273"
ORIGIN      
        1 mivqrclsfa vpiclilsie fgtiegerws rqvsnsewip lanpratqtq ieqnnptgll
       61 asgsaqvpqv pqlslppplq qqyqeqllql qktqesiqkl lllqqqlktq qqllqsqtfl
      121 psgfssqeed kqlrqsgltn sqtlpdvase dlvpplpase alppvfpaqn ffqqrprppp
      181 tpskqdlpqe flnlsgqefk sgqllpqgqn lgnvgqiden qrqgakhlge tlpqgvnaqd
      241 eeeevqlvyv padtlaqrgq pkrgrgrkqy phrqqqqhhr dnqgvdtsai apdqdaftrq
      301 llqqiqieek aqffneerlk eiarlneeqr elernarlqe eamrreqelq rqreaekkrk
      361 eleeiekqre lerirearek qrleeleqrr laeirakeek rraeeaarrr eaeeaarrre
      421 aeeaarqrei eeaarqreae eaarqrever laalerkkeq emqralkqqq llekqreeea
      481 rvsrlrnenq eppaqalpli kdqqqqfhrq hltetskpvk srirgrtrqr phhqdqqnyr
      541 ettttpapsp nqpplsvymg shssktanvk vsdvlkilkd aktiavldtv gpdtpqvfvg
      601 ptnldpptgy akfdlpylss idhnrverkv dklpffvapl sfdpppgysk ipfpaphigs
      661 vvvntldntd pkdsdnpnps ptpliepnsy ldglnsgsds ttplyepqtt igytqehsst
      721 pkyeqtyssa pptgysgsrf rfrqyysdsk pasvvttsyy ddqrpttrky kyyednvrtt
      781 dnplintrve tsvsqqeels ysttprikde sistqdpstk aqdiaaqlal inqelaqqre
      841 tqryntagqf nrynsqsttg lghsfdgeia sgdindvrgp igptqynlpa elpaisphlp
      901 glvnslldkn egklktnapa tttttttttt tttqrtpttt yrprsrqrsr vastrprttt
      961 qtpstrtnvd rsrrpynrsr srfttttedy resatyeatk sriaettvry steqrrptsr
     1021 tqkhrgrdka sqqaealqnq niqqsyenvp hgdsaspats ntflqptapd snlrqadlss
     1081 ntpenypsna isttenypsp lpanqaspli sedysrsqny pqnlpqdphy rggydqpale
     1141 qsgletapvn gfnyqtqsrd tfeqeivqrp kdyqldgkae nsdielatta sprlgaqeeq
     1201 ryssvyevnv pqtqaeyglt gqnnlhrles ekiqpgaadp dnpvfillpe nkqedyelsv
     1261 ssteapisdi tteatttstt pviirqrvrg rvgsrvhhes svqtrnrgsq deyvrfnvvn
     1321 qestrtspsr qrgrsrsrtq nhgsqvetdg neyikihvaq qqkqvatttp lppttttttt
     1381 tttttvrpte edidygfirp psfrpvhpvd nrfqapvtfr pqlsehllqa phqsllpnde
     1441 tamelsppqt qllktrqkyq sapnrrlpgk attlpppttt ietiasterv pvatllpdds
     1501 vyterpkprt deskqsrirs rvrrpgrkrv sssstttesv leahnelpld enyprirpqs
     1561 tsteqahqqn lyddnydggp qfppvrnpes qqfyetssen ypaefvlnfg nypsqpvqre
     1621 eydqtqlase lprrysqatg akttsedsrs ttvdiygaes qwstkltrts fqpsfavnrv
     1681 hgeakndrew fhesskdsnv pdiitaapev ssvtlvvssd dkkseeteed eeesmlmgtt
     1741 ifgrktntqd htegmnasdt ttetpstimr erelkrdpqs wlmdrvesst eqrgdidfek
     1801 sqkrigdggv vkkgtrrrrv rvrvrpavdd fvtaesqhfn salngifrdq ykynsvresk
     1861 hsassnsdkf dqqdfeslkn ndpakstste vpeaawtmpp aittpmsipd dteettteeq
     1921 ittlpttnqp edttitqssy edfvsrftes sdtvetsdep kkkkdnwrdk svrvtlnlgs
     1981 iadamkendv nklsdgsnea trdeklnkve kessvenees neenddhpkn hrakwsevry
     2041 pssldhpqas gwkyerktgg ksssipglvt kqegdnsvkt lsdyvqaifd tmksteeett
     2101 iaeaerpees ttlptpfdne fvesqerttt kdvedkttar atldeeqqtt rnvdvngkve
     2161 stepttnidl enattsekvt tsqesvtsss seystdlptt tpprttpsts nstetilgki
     2221 lrtstttkvs hmteicyrgr cvmtrpsrev rlr
//
LOCUS       XP_034178164            2244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603279 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178164
VERSION     XP_034178164.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322273.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178164.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2244
                     /product="uncharacterized protein LOC117603279 isoform X1"
                     /calculated_mol_wt=251925
     Region          1442..1560
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          1566..1629
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          <1813..2090
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..2244
                     /gene="LOC117603279"
                     /coded_by="XM_034322273.2:265..6999"
                     /db_xref="GeneID:117603279"
ORIGIN      
        1 maggrgnnkq sgnldrihqw lychqlyasv klelplskif kkhqckeesr ivtkslrdlh
       61 dnilsiftds lycdfniiyg hniirtnqci iktraphfyk vlkpyfmrin nhidciidkl
      121 eifqhiesfv rllytnsnls kaeqslveyi lsdsklidts saikisdane ryvdyasadr
      181 pltsikstrn vreyapadvs ptlsdmadsg letgsvsphe nttsensstd feelqvteyn
      241 stnddidkir arrvnnkdqk lvidnehyns snynstchlk dlvesglmec asndhsatcs
      301 sfdaeqpqld ssssstenvp kakphtdeif qyenqvlsdp fyesdcgsde nesfefidlg
      361 nasslstdae rtsakedste klqdenlcqs nysqadnaqs envndgasnd ttkqksssnt
      421 snyyfidast lndeievpas nvvknhcfdd kpqsytsytf kyvtsksndf vdeqcykftp
      481 lkatnlqtgh ekvaefqrel klteylepla dtqkvkrtds isedkinlnt etnkeslvve
      541 ikeadsgesa hpspcpdnnt ninndrhvas rtnnpsdsva vyteketdeq tnnnnnnnnn
      601 nsvedtrrps lirrntfeld sndeklsvlr qeyerrqgnl vfqnaipqys ghrvdgdscf
      661 ippeepsipm nssvlkkftm evqvssdpqy hpvpylsldn tkyesdhtpp dadsflenkp
      721 ntvypvtksa sdeividhha eldndssncs nslpvtlsya iendrtdtmk kikskcdeta
      781 piisggvsts dyskptdspt vrrktestpi vsggsvimne pdlrikptrm sssmtawvvd
      841 msdcnkndpk ssnvhtgmsq sfstsecmkr parkisnhdk hgslgffvnl kdmdkkpaae
      901 qnqptekkeq ngcsksycef yvdmsnates lklkkpeiee padkceksne gndkknifsm
      961 fidlsdppkn pesnvqlshr rsfsaftdkq teaksddsns sknssitare dqssteqkcv
     1021 rekakpsvfm yiesdspvvr rrtlstsrpa fkrhswnvdk tqsvnnngqv akelmfrkeh
     1081 krahslsvdr gdlkkfqgkp nsstnslsdq tkpesipqkn pkllqegnnv tnmdtssedc
     1141 feydvrdtpp nshveiinee lrvsikehey telktertte slnvgdvkay edeysetsgw
     1201 ektctesteg qtrksetfdi ssgsgpspds dnhdyelsel lngevpnmdr sqvvpavgsk
     1261 isethkslne tikkieselk npdyvqteat ydnhnaiskk servagnnlk ttssnfvrls
     1321 dldktpvash sadilcvked rstyrvrnni setswienkl lisrtngsvr ppprkfpsim
     1381 stslppkqks pleeltgeyd gegiisesdl ssmqssmgrs gaegsteete tsslagsrpy
     1441 nrlgedllrm fleeinpdvt idvagrrira hkcilssrcq yfaailsggw iesagnvisl
     1501 qgysydavhf alchiysges nipdsisive latladmlcl eglkeiigyt lkvkychlfh
     1561 kpcqicavgv lecmplaaay gldevyrksl rwvtrhfvri wpckafatlp kelmakcyhq
     1621 hivhmstdnv lqtmmdcdkl latlpnvrwa epvfrmvsnl letsvkflsd nfsgvlgnen
     1681 fqslgreltw nisrlednfl aaaerlppdq acksysklhk mlasvqqdea qekfkwgplf
     1741 idflkkiqsr vekclvrdaa raartttwlk mdlelrrriq elaclvilpr etikrqpknp
     1801 nfikeprpts srsmtnrsld lkrvkmvise hnhktlkqma vvqqqqtkkv fnkpksdple
     1861 rkmqndkqit ttetnrpksw pnklevksry leprnksvpk etvqppqqek vmvqqrrkim
     1921 isssdssrts spamkratdk kplakiklpi kkdvkalssd sltesnatrt nnnkkdsisk
     1981 scgitrpesp sfkqknteig lsvdslaesk nkpavvkkkt nkmdtsmstd slmtemtttp
     2041 ksntsnklsp tlgkaankaq vfdkgakkss pptqqrsplt alrrprksle sstaasrsra
     2101 aaisayhlrr nlldaaktpd vpskslnnvs ykttstrpvs qstvnhpsvt rekkesvlnq
     2161 qgsespskrs spkssgtnki skptvvnkri tgnskascde ksknkchnge vpkqptvgsr
     2221 sgtflkdept ilkkadikss qint
//
LOCUS       XP_034178179            2244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603279 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178179
VERSION     XP_034178179.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322288.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178179.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2244
                     /product="uncharacterized protein LOC117603279 isoform X1"
                     /calculated_mol_wt=251925
     Region          1442..1560
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          1566..1629
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          <1813..2090
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..2244
                     /gene="LOC117603279"
                     /coded_by="XM_034322288.2:374..7108"
                     /db_xref="GeneID:117603279"
ORIGIN      
        1 maggrgnnkq sgnldrihqw lychqlyasv klelplskif kkhqckeesr ivtkslrdlh
       61 dnilsiftds lycdfniiyg hniirtnqci iktraphfyk vlkpyfmrin nhidciidkl
      121 eifqhiesfv rllytnsnls kaeqslveyi lsdsklidts saikisdane ryvdyasadr
      181 pltsikstrn vreyapadvs ptlsdmadsg letgsvsphe nttsensstd feelqvteyn
      241 stnddidkir arrvnnkdqk lvidnehyns snynstchlk dlvesglmec asndhsatcs
      301 sfdaeqpqld ssssstenvp kakphtdeif qyenqvlsdp fyesdcgsde nesfefidlg
      361 nasslstdae rtsakedste klqdenlcqs nysqadnaqs envndgasnd ttkqksssnt
      421 snyyfidast lndeievpas nvvknhcfdd kpqsytsytf kyvtsksndf vdeqcykftp
      481 lkatnlqtgh ekvaefqrel klteylepla dtqkvkrtds isedkinlnt etnkeslvve
      541 ikeadsgesa hpspcpdnnt ninndrhvas rtnnpsdsva vyteketdeq tnnnnnnnnn
      601 nsvedtrrps lirrntfeld sndeklsvlr qeyerrqgnl vfqnaipqys ghrvdgdscf
      661 ippeepsipm nssvlkkftm evqvssdpqy hpvpylsldn tkyesdhtpp dadsflenkp
      721 ntvypvtksa sdeividhha eldndssncs nslpvtlsya iendrtdtmk kikskcdeta
      781 piisggvsts dyskptdspt vrrktestpi vsggsvimne pdlrikptrm sssmtawvvd
      841 msdcnkndpk ssnvhtgmsq sfstsecmkr parkisnhdk hgslgffvnl kdmdkkpaae
      901 qnqptekkeq ngcsksycef yvdmsnates lklkkpeiee padkceksne gndkknifsm
      961 fidlsdppkn pesnvqlshr rsfsaftdkq teaksddsns sknssitare dqssteqkcv
     1021 rekakpsvfm yiesdspvvr rrtlstsrpa fkrhswnvdk tqsvnnngqv akelmfrkeh
     1081 krahslsvdr gdlkkfqgkp nsstnslsdq tkpesipqkn pkllqegnnv tnmdtssedc
     1141 feydvrdtpp nshveiinee lrvsikehey telktertte slnvgdvkay edeysetsgw
     1201 ektctesteg qtrksetfdi ssgsgpspds dnhdyelsel lngevpnmdr sqvvpavgsk
     1261 isethkslne tikkieselk npdyvqteat ydnhnaiskk servagnnlk ttssnfvrls
     1321 dldktpvash sadilcvked rstyrvrnni setswienkl lisrtngsvr ppprkfpsim
     1381 stslppkqks pleeltgeyd gegiisesdl ssmqssmgrs gaegsteete tsslagsrpy
     1441 nrlgedllrm fleeinpdvt idvagrrira hkcilssrcq yfaailsggw iesagnvisl
     1501 qgysydavhf alchiysges nipdsisive latladmlcl eglkeiigyt lkvkychlfh
     1561 kpcqicavgv lecmplaaay gldevyrksl rwvtrhfvri wpckafatlp kelmakcyhq
     1621 hivhmstdnv lqtmmdcdkl latlpnvrwa epvfrmvsnl letsvkflsd nfsgvlgnen
     1681 fqslgreltw nisrlednfl aaaerlppdq acksysklhk mlasvqqdea qekfkwgplf
     1741 idflkkiqsr vekclvrdaa raartttwlk mdlelrrriq elaclvilpr etikrqpknp
     1801 nfikeprpts srsmtnrsld lkrvkmvise hnhktlkqma vvqqqqtkkv fnkpksdple
     1861 rkmqndkqit ttetnrpksw pnklevksry leprnksvpk etvqppqqek vmvqqrrkim
     1921 isssdssrts spamkratdk kplakiklpi kkdvkalssd sltesnatrt nnnkkdsisk
     1981 scgitrpesp sfkqknteig lsvdslaesk nkpavvkkkt nkmdtsmstd slmtemtttp
     2041 ksntsnklsp tlgkaankaq vfdkgakkss pptqqrsplt alrrprksle sstaasrsra
     2101 aaisayhlrr nlldaaktpd vpskslnnvs ykttstrpvs qstvnhpsvt rekkesvlnq
     2161 qgsespskrs spkssgtnki skptvvnkri tgnskascde ksknkchnge vpkqptvgsr
     2221 sgtflkdept ilkkadikss qint
//
LOCUS       XP_034178173            2244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603279 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178173
VERSION     XP_034178173.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322282.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178173.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2244
                     /product="uncharacterized protein LOC117603279 isoform X1"
                     /calculated_mol_wt=251925
     Region          1442..1560
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          1566..1629
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          <1813..2090
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..2244
                     /gene="LOC117603279"
                     /coded_by="XM_034322282.2:255..6989"
                     /db_xref="GeneID:117603279"
ORIGIN      
        1 maggrgnnkq sgnldrihqw lychqlyasv klelplskif kkhqckeesr ivtkslrdlh
       61 dnilsiftds lycdfniiyg hniirtnqci iktraphfyk vlkpyfmrin nhidciidkl
      121 eifqhiesfv rllytnsnls kaeqslveyi lsdsklidts saikisdane ryvdyasadr
      181 pltsikstrn vreyapadvs ptlsdmadsg letgsvsphe nttsensstd feelqvteyn
      241 stnddidkir arrvnnkdqk lvidnehyns snynstchlk dlvesglmec asndhsatcs
      301 sfdaeqpqld ssssstenvp kakphtdeif qyenqvlsdp fyesdcgsde nesfefidlg
      361 nasslstdae rtsakedste klqdenlcqs nysqadnaqs envndgasnd ttkqksssnt
      421 snyyfidast lndeievpas nvvknhcfdd kpqsytsytf kyvtsksndf vdeqcykftp
      481 lkatnlqtgh ekvaefqrel klteylepla dtqkvkrtds isedkinlnt etnkeslvve
      541 ikeadsgesa hpspcpdnnt ninndrhvas rtnnpsdsva vyteketdeq tnnnnnnnnn
      601 nsvedtrrps lirrntfeld sndeklsvlr qeyerrqgnl vfqnaipqys ghrvdgdscf
      661 ippeepsipm nssvlkkftm evqvssdpqy hpvpylsldn tkyesdhtpp dadsflenkp
      721 ntvypvtksa sdeividhha eldndssncs nslpvtlsya iendrtdtmk kikskcdeta
      781 piisggvsts dyskptdspt vrrktestpi vsggsvimne pdlrikptrm sssmtawvvd
      841 msdcnkndpk ssnvhtgmsq sfstsecmkr parkisnhdk hgslgffvnl kdmdkkpaae
      901 qnqptekkeq ngcsksycef yvdmsnates lklkkpeiee padkceksne gndkknifsm
      961 fidlsdppkn pesnvqlshr rsfsaftdkq teaksddsns sknssitare dqssteqkcv
     1021 rekakpsvfm yiesdspvvr rrtlstsrpa fkrhswnvdk tqsvnnngqv akelmfrkeh
     1081 krahslsvdr gdlkkfqgkp nsstnslsdq tkpesipqkn pkllqegnnv tnmdtssedc
     1141 feydvrdtpp nshveiinee lrvsikehey telktertte slnvgdvkay edeysetsgw
     1201 ektctesteg qtrksetfdi ssgsgpspds dnhdyelsel lngevpnmdr sqvvpavgsk
     1261 isethkslne tikkieselk npdyvqteat ydnhnaiskk servagnnlk ttssnfvrls
     1321 dldktpvash sadilcvked rstyrvrnni setswienkl lisrtngsvr ppprkfpsim
     1381 stslppkqks pleeltgeyd gegiisesdl ssmqssmgrs gaegsteete tsslagsrpy
     1441 nrlgedllrm fleeinpdvt idvagrrira hkcilssrcq yfaailsggw iesagnvisl
     1501 qgysydavhf alchiysges nipdsisive latladmlcl eglkeiigyt lkvkychlfh
     1561 kpcqicavgv lecmplaaay gldevyrksl rwvtrhfvri wpckafatlp kelmakcyhq
     1621 hivhmstdnv lqtmmdcdkl latlpnvrwa epvfrmvsnl letsvkflsd nfsgvlgnen
     1681 fqslgreltw nisrlednfl aaaerlppdq acksysklhk mlasvqqdea qekfkwgplf
     1741 idflkkiqsr vekclvrdaa raartttwlk mdlelrrriq elaclvilpr etikrqpknp
     1801 nfikeprpts srsmtnrsld lkrvkmvise hnhktlkqma vvqqqqtkkv fnkpksdple
     1861 rkmqndkqit ttetnrpksw pnklevksry leprnksvpk etvqppqqek vmvqqrrkim
     1921 isssdssrts spamkratdk kplakiklpi kkdvkalssd sltesnatrt nnnkkdsisk
     1981 scgitrpesp sfkqknteig lsvdslaesk nkpavvkkkt nkmdtsmstd slmtemtttp
     2041 ksntsnklsp tlgkaankaq vfdkgakkss pptqqrsplt alrrprksle sstaasrsra
     2101 aaisayhlrr nlldaaktpd vpskslnnvs ykttstrpvs qstvnhpsvt rekkesvlnq
     2161 qgsespskrs spkssgtnki skptvvnkri tgnskascde ksknkchnge vpkqptvgsr
     2221 sgtflkdept ilkkadikss qint
//
LOCUS       XP_034178184            2244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603279 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178184
VERSION     XP_034178184.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322293.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178184.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2244
                     /product="uncharacterized protein LOC117603279 isoform X1"
                     /calculated_mol_wt=251925
     Region          1442..1560
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          1566..1629
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          <1813..2090
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..2244
                     /gene="LOC117603279"
                     /coded_by="XM_034322293.2:168..6902"
                     /db_xref="GeneID:117603279"
ORIGIN      
        1 maggrgnnkq sgnldrihqw lychqlyasv klelplskif kkhqckeesr ivtkslrdlh
       61 dnilsiftds lycdfniiyg hniirtnqci iktraphfyk vlkpyfmrin nhidciidkl
      121 eifqhiesfv rllytnsnls kaeqslveyi lsdsklidts saikisdane ryvdyasadr
      181 pltsikstrn vreyapadvs ptlsdmadsg letgsvsphe nttsensstd feelqvteyn
      241 stnddidkir arrvnnkdqk lvidnehyns snynstchlk dlvesglmec asndhsatcs
      301 sfdaeqpqld ssssstenvp kakphtdeif qyenqvlsdp fyesdcgsde nesfefidlg
      361 nasslstdae rtsakedste klqdenlcqs nysqadnaqs envndgasnd ttkqksssnt
      421 snyyfidast lndeievpas nvvknhcfdd kpqsytsytf kyvtsksndf vdeqcykftp
      481 lkatnlqtgh ekvaefqrel klteylepla dtqkvkrtds isedkinlnt etnkeslvve
      541 ikeadsgesa hpspcpdnnt ninndrhvas rtnnpsdsva vyteketdeq tnnnnnnnnn
      601 nsvedtrrps lirrntfeld sndeklsvlr qeyerrqgnl vfqnaipqys ghrvdgdscf
      661 ippeepsipm nssvlkkftm evqvssdpqy hpvpylsldn tkyesdhtpp dadsflenkp
      721 ntvypvtksa sdeividhha eldndssncs nslpvtlsya iendrtdtmk kikskcdeta
      781 piisggvsts dyskptdspt vrrktestpi vsggsvimne pdlrikptrm sssmtawvvd
      841 msdcnkndpk ssnvhtgmsq sfstsecmkr parkisnhdk hgslgffvnl kdmdkkpaae
      901 qnqptekkeq ngcsksycef yvdmsnates lklkkpeiee padkceksne gndkknifsm
      961 fidlsdppkn pesnvqlshr rsfsaftdkq teaksddsns sknssitare dqssteqkcv
     1021 rekakpsvfm yiesdspvvr rrtlstsrpa fkrhswnvdk tqsvnnngqv akelmfrkeh
     1081 krahslsvdr gdlkkfqgkp nsstnslsdq tkpesipqkn pkllqegnnv tnmdtssedc
     1141 feydvrdtpp nshveiinee lrvsikehey telktertte slnvgdvkay edeysetsgw
     1201 ektctesteg qtrksetfdi ssgsgpspds dnhdyelsel lngevpnmdr sqvvpavgsk
     1261 isethkslne tikkieselk npdyvqteat ydnhnaiskk servagnnlk ttssnfvrls
     1321 dldktpvash sadilcvked rstyrvrnni setswienkl lisrtngsvr ppprkfpsim
     1381 stslppkqks pleeltgeyd gegiisesdl ssmqssmgrs gaegsteete tsslagsrpy
     1441 nrlgedllrm fleeinpdvt idvagrrira hkcilssrcq yfaailsggw iesagnvisl
     1501 qgysydavhf alchiysges nipdsisive latladmlcl eglkeiigyt lkvkychlfh
     1561 kpcqicavgv lecmplaaay gldevyrksl rwvtrhfvri wpckafatlp kelmakcyhq
     1621 hivhmstdnv lqtmmdcdkl latlpnvrwa epvfrmvsnl letsvkflsd nfsgvlgnen
     1681 fqslgreltw nisrlednfl aaaerlppdq acksysklhk mlasvqqdea qekfkwgplf
     1741 idflkkiqsr vekclvrdaa raartttwlk mdlelrrriq elaclvilpr etikrqpknp
     1801 nfikeprpts srsmtnrsld lkrvkmvise hnhktlkqma vvqqqqtkkv fnkpksdple
     1861 rkmqndkqit ttetnrpksw pnklevksry leprnksvpk etvqppqqek vmvqqrrkim
     1921 isssdssrts spamkratdk kplakiklpi kkdvkalssd sltesnatrt nnnkkdsisk
     1981 scgitrpesp sfkqknteig lsvdslaesk nkpavvkkkt nkmdtsmstd slmtemtttp
     2041 ksntsnklsp tlgkaankaq vfdkgakkss pptqqrsplt alrrprksle sstaasrsra
     2101 aaisayhlrr nlldaaktpd vpskslnnvs ykttstrpvs qstvnhpsvt rekkesvlnq
     2161 qgsespskrs spkssgtnki skptvvnkri tgnskascde ksknkchnge vpkqptvgsr
     2221 sgtflkdept ilkkadikss qint
//
LOCUS       XP_034178193            2243 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603279 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178193
VERSION     XP_034178193.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322302.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178193.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2243
                     /product="uncharacterized protein LOC117603279 isoform X2"
                     /calculated_mol_wt=251796
     Region          1441..1559
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          1565..1628
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          <1812..2089
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..2243
                     /gene="LOC117603279"
                     /coded_by="XM_034322302.2:265..6996"
                     /db_xref="GeneID:117603279"
ORIGIN      
        1 maggrgnnkq sgnldrihqw lychqlyasv klelplskif kkhqckeesr ivtkslrdlh
       61 dnilsiftds lycdfniiyg hniirtnqci iktraphfyk vlkpyfmrin nhidciidkl
      121 eifqhiesfv rllytnsnls kaeqslveyi lsdsklidts saikisdane ryvdyasadr
      181 pltsikstrn vreyapadvs ptlsdmadsg letgsvsphe nttsensstd feelqvteyn
      241 stnddidkir arrvnnkdqk lvidnehyns snynstchlk dlvesglmec asndhsatcs
      301 sfdaeqpqld ssssstenvp kakphtdeif qyenqvlsdp fyesdcgsde nesfefidlg
      361 nasslstdae rtsakedste klqdenlcqs nysqadnaqs envndgasnd ttkqksssnt
      421 snyyfidast lndeievpas nvvknhcfdd kpqsytsytf kyvtsksndf vdeqcykftp
      481 lkatnlqtgh ekvaefqrel klteylepla dtqkvkrtds isedkinlnt etnkeslvve
      541 ikeadsgesa hpspcpdnnt ninndrhvas rtnnpsdsva vyteketdeq tnnnnnnnnn
      601 nsvedtrrps lirrntfeld sndeklsvlr qeyerrqgnl vfqnaipqys ghrvdgdscf
      661 ippeepsipm nssvlkkftm evqvssdpqy hpvpylsldn tkyesdhtpp dadsflenkp
      721 ntvypvtksa sdeividhha eldndssncs nslpvtlsya iendrtdtmk kikskcdeta
      781 piisggvsts dyskptdspt vrrktestpi vsggsvimne pdlrikptrm sssmtawvvd
      841 msdcnkndpk ssnvhtgmsq sfstsecmkr parkisnhdk hgslgffvnl kdmdkkpaae
      901 qnqptekkeq ngcsksycef yvdmsnates lklkkpeiee padkceksne gndkknifsm
      961 fidlsdppkn pesnvqlshr rsfsaftdkq teaksddsns sknssitare dqssteqkcv
     1021 rekakpsvfm yiesdspvvr rrtlstsrpa fkrhswnvdk tqsvnnngqv akelmfrkeh
     1081 krahslsvdr gdlkkfqgkp nsstnslsdq tkpesipqkn pkllqegnnv tnmdtssedc
     1141 feydvrdtpp nshveiinee lrvsikehey telktertte slnvgdvkay edeysetsgw
     1201 ektctesteg qtrksetfdi ssgsgpspds dnhdyelsel lngevpnmdr sqvvpavgsk
     1261 isethkslne tikkieselk npdyvqteat ydnhnaiskk servagnnlk ttssnfvrls
     1321 dldktpvash sadilcvked rstyrvrnni setswienkl lisrtngsvr ppprkfpsim
     1381 stslppkqks pleeltgeyd gegiisesdl ssmqssmgrs gagsteetet sslagsrpyn
     1441 rlgedllrmf leeinpdvti dvagrrirah kcilssrcqy faailsggwi esagnvislq
     1501 gysydavhfa lchiysgesn ipdsisivel atladmlcle glkeiigytl kvkychlfhk
     1561 pcqicavgvl ecmplaaayg ldevyrkslr wvtrhfvriw pckafatlpk elmakcyhqh
     1621 ivhmstdnvl qtmmdcdkll atlpnvrwae pvfrmvsnll etsvkflsdn fsgvlgnenf
     1681 qslgreltwn isrlednfla aaerlppdqa cksysklhkm lasvqqdeaq ekfkwgplfi
     1741 dflkkiqsrv ekclvrdaar aartttwlkm dlelrrriqe laclvilpre tikrqpknpn
     1801 fikeprptss rsmtnrsldl krvkmviseh nhktlkqmav vqqqqtkkvf nkpksdpler
     1861 kmqndkqitt tetnrpkswp nklevksryl eprnksvpke tvqppqqekv mvqqrrkimi
     1921 sssdssrtss pamkratdkk plakiklpik kdvkalssds ltesnatrtn nnkkdsisks
     1981 cgitrpesps fkqknteigl svdslaeskn kpavvkkktn kmdtsmstds lmtemtttpk
     2041 sntsnklspt lgkaankaqv fdkgakkssp ptqqrsplta lrrprksles staasrsraa
     2101 aisayhlrrn lldaaktpdv pskslnnvsy kttstrpvsq stvnhpsvtr ekkesvlnqq
     2161 gsespskrss pkssgtnkis kptvvnkrit gnskascdek sknkchngev pkqptvgsrs
     2221 gtflkdepti lkkadikssq int
//
LOCUS       XP_034178202            2178 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603279 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178202
VERSION     XP_034178202.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178202.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2178
                     /product="uncharacterized protein LOC117603279 isoform X3"
                     /calculated_mol_wt=243978
     Region          1376..1494
                     /region_name="BTB2_POZ_BTBD8"
                     /note="second BTB (Broad-Complex, Tramtrack and Bric a
                     brac)/POZ (poxvirus and zinc finger) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18286"
                     /db_xref="CDD:349595"
     Region          1500..1563
                     /region_name="BACK_BTBD8"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing protein 8 (BTBD8); cd18490"
                     /db_xref="CDD:350565"
     Region          <1747..2024
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..2178
                     /gene="LOC117603279"
                     /coded_by="XM_034322311.2:264..6800"
                     /db_xref="GeneID:117603279"
ORIGIN      
        1 maggrgnnkq sgnldrihqw lychqlyasv klelplskif kkhqckeesr ivtkslrdlh
       61 dnilrllytn snlskaeqsl veyilsdskl idtssaikis daneryvdya sadrpltsik
      121 strnvreyap advsptlsdm adsgletgsv sphenttsen sstdfeelqv teynstnddi
      181 dkirarrvnn kdqklvidne hynssnynst chlkdlvesg lmecasndhs atcssfdaeq
      241 pqldssssst envpkakpht deifqyenqv lsdpfyesdc gsdenesfef idlgnassls
      301 tdaertsake dsteklqden lcqsnysqad naqsenvndg asndttkqks ssntsnyyfi
      361 dastlndeie vpasnvvknh cfddkpqsyt sytfkyvtsk sndfvdeqcy kftplkatnl
      421 qtghekvaef qrelklteyl epladtqkvk rtdsisedki nlntetnkes lvveikeads
      481 gesahpspcp dnntninndr hvasrtnnps dsvavyteke tdeqtnnnnn nnnnnsvedt
      541 rrpslirrnt feldsndekl svlrqeyerr qgnlvfqnai pqysghrvdg dscfippeep
      601 sipmnssvlk kftmevqvss dpqyhpvpyl sldntkyesd htppdadsfl enkpntvypv
      661 tksasdeivi dhhaeldnds sncsnslpvt lsyaiendrt dtmkkikskc detapiisgg
      721 vstsdyskpt dsptvrrkte stpivsggsv imnepdlrik ptrmsssmta wvvdmsdcnk
      781 ndpkssnvht gmsqsfstse cmkrparkis nhdkhgslgf fvnlkdmdkk paaeqnqpte
      841 kkeqngcsks ycefyvdmsn ateslklkkp eieepadkce ksnegndkkn ifsmfidlsd
      901 ppknpesnvq lshrrsfsaf tdkqteaksd dsnssknssi taredqsste qkcvrekakp
      961 svfmyiesds pvvrrrtlst srpafkrhsw nvdktqsvnn ngqvakelmf rkehkrahsl
     1021 svdrgdlkkf qgkpnsstns lsdqtkpesi pqknpkllqe gnnvtnmdts sedcfeydvr
     1081 dtppnshvei ineelrvsik eheytelkte rtteslnvgd vkayedeyse tsgwektcte
     1141 stegqtrkse tfdissgsgp spdsdnhdye lsellngevp nmdrsqvvpa vgskisethk
     1201 slnetikkie selknpdyvq teatydnhna iskkservag nnlkttssnf vrlsdldktp
     1261 vashsadilc vkedrstyrv rnnisetswi enkllisrtn gsvrppprkf psimstslpp
     1321 kqkspleelt geydgegiis esdlssmqss mgrsgaegst eetetsslag srpynrlged
     1381 llrmfleein pdvtidvagr rirahkcils srcqyfaail sggwiesagn vislqgysyd
     1441 avhfalchiy sgesnipdsi sivelatlad mlcleglkei igytlkvkyc hlfhkpcqic
     1501 avgvlecmpl aaaygldevy rkslrwvtrh fvriwpckaf atlpkelmak cyhqhivhms
     1561 tdnvlqtmmd cdkllatlpn vrwaepvfrm vsnlletsvk flsdnfsgvl gnenfqslgr
     1621 eltwnisrle dnflaaaerl ppdqacksys klhkmlasvq qdeaqekfkw gplfidflkk
     1681 iqsrvekclv rdaaraartt twlkmdlelr rriqelaclv ilpretikrq pknpnfikep
     1741 rptssrsmtn rsldlkrvkm visehnhktl kqmavvqqqq tkkvfnkpks dplerkmqnd
     1801 kqitttetnr pkswpnklev ksryleprnk svpketvqpp qqekvmvqqr rkimisssds
     1861 srtsspamkr atdkkplaki klpikkdvka lssdsltesn atrtnnnkkd siskscgitr
     1921 pespsfkqkn teiglsvdsl aesknkpavv kkktnkmdts mstdslmtem tttpksntsn
     1981 klsptlgkaa nkaqvfdkga kksspptqqr spltalrrpr kslesstaas rsraaaisay
     2041 hlrrnlldaa ktpdvpsksl nnvsykttst rpvsqstvnh psvtrekkes vlnqqgsesp
     2101 skrsspkssg tnkiskptvv nkritgnska scdeksknkc hngevpkqpt vgsrsgtflk
     2161 deptilkkad ikssqint
//
LOCUS       XP_034178217            1128 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 8 white walker isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034178217
VERSION     XP_034178217.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322326.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1128
                     /product="anoctamin 8 white walker isoform X1"
                     /calculated_mol_wt=125618
     Region          269..751
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     Region          999..>1089
                     /region_name="CAF-1_p150"
                     /note="Chromatin assembly factor 1 complex p150 subunit,
                     N-terminal; pfam11600"
                     /db_xref="CDD:402959"
     CDS             1..1128
                     /gene="wwk"
                     /coded_by="XM_034322326.2:46..3432"
                     /db_xref="GeneID:117603311"
ORIGIN      
        1 mpggegspin lldhrtstts gecansdede rttatasstm ttvsggdglr rrkvihaake
       61 tldkasrllr rkipctghlm tprrlwiqkv ptqecdvvmm fpsgasdetl mwllgrlrag
      121 tpglvvhvrh hassdsysfy ltapfsvllk aaeevhlpkt lrqefggglk efvgtesscf
      181 egsddearff ttqerqslvl hllhtlragp qdlhslsglk mvegqaiipk cisagiisqv
      241 fplhelpale klqrtwvraf lspqplddic kyfgvkitmy fawlghytta livpaavgai
      301 ywvgiigrnq avedvayvlf svfnviwatv yletwkrrga elayrwgtld qrddllvepr
      361 plftgtleis hvtgrlepty prwkrnmfry fvsvpviaac lffvfivmil sfqiqdwwda
      421 rlesrgygfw lsyvpkvlla vvialmdeay fkvavwlndm enyrldteye nhliykvalf
      481 qfvnsflslf yiafylqdqe rlkeqlaall iarqvignlk esavpylieq lrlarlsfel
      541 fgalspsear sppgeenade nqkdaedkse rsesgkstqp rnvsqaeles slyrvghptn
      601 sllsdvtfkv stkkydgafs ehlemlsqlg yvclfssafp laamaallgn llelrgdafk
      661 lcfvlqrpfg rrvsnigtwq nameamglva ivvncaligl sgqvqrmfpe msatqtilli
      721 valehimlgi rfiiicaipd iphwvatema kveflrreav rrlsstpspe qqpatvigrf
      781 vvspadgese eqqlltdrtg dattsslpdt ptlastthtp sspptpsaas vprisetpsa
      841 aqtpgsagss digspfltdn gmgsvrdlhn sprcsipgge rgrrsrewla tepeasggsd
      901 hyshhltigp hggvdwvrrl glepgarkss dseiggsgtv sgsgdeltlh rstdcivske
      961 lasssdsdll rsappwtvah rnqkfrfspe rerereraek lqyqqhqrdi qdhkqqaaek
     1021 ekesgalsds sktissqeee kptkedreak ktrvkqslmk rarsvaifsl klkerrarea
     1081 elkakeaeke arwqqpqscv ggelscipie klisvddiaa melrrlnh
//
LOCUS       XP_034178226            1123 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 8 white walker isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034178226
VERSION     XP_034178226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322335.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1123
                     /product="anoctamin 8 white walker isoform X2"
                     /calculated_mol_wt=125074
     Region          269..746
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     Region          994..>1084
                     /region_name="CAF-1_p150"
                     /note="Chromatin assembly factor 1 complex p150 subunit,
                     N-terminal; pfam11600"
                     /db_xref="CDD:402959"
     CDS             1..1123
                     /gene="wwk"
                     /coded_by="XM_034322335.2:46..3417"
                     /db_xref="GeneID:117603311"
ORIGIN      
        1 mpggegspin lldhrtstts gecansdede rttatasstm ttvsggdglr rrkvihaake
       61 tldkasrllr rkipctghlm tprrlwiqkv ptqecdvvmm fpsgasdetl mwllgrlrag
      121 tpglvvhvrh hassdsysfy ltapfsvllk aaeevhlpkt lrqefggglk efvgtesscf
      181 egsddearff ttqerqslvl hllhtlragp qdlhslsglk mvegqaiipk cisagiisqv
      241 fplhelpale klqrtwvraf lspqplddic kyfgvkitmy fawlghytta livpaavgai
      301 ywvgiigrnq avedvayvlf svfnviwatv yletwkrrga elayrwgtld qrddllvepr
      361 plftgtleis hvtgrlepty prwkrnmfry fvsvpviaac lffvfivmil sfqiqdwwda
      421 rlesrgygfw lsyvpkvlla vvialmdeay fkvavwlndm enyrldteye nhliykvalf
      481 qfvnsflslf yiafylqdqe rlkeqlaall iarqvignlk esavpylieq lrlarlsfel
      541 fgalspsear sppgeenade nqkdaedkse rsesgkstqp rnvsqaeles slyrvghptn
      601 sllsdvtfky dgafsehlem lsqlgyvclf ssafplaama allgnllelr gdafklcfvl
      661 qrpfgrrvsn igtwqnamea mglvaivvnc aliglsgqvq rmfpemsatq tillivaleh
      721 imlgirfiii caipdiphwv atemakvefl rreavrrlss tpspeqqpat vigrfvvspa
      781 dgeseeqqll tdrtgdatts slpdtptlas tthtpssppt psaasvpris etpsaaqtpg
      841 sagssdigsp fltdngmgsv rdlhnsprcs ipggergrrs rewlatepea sggsdhyshh
      901 ltigphggvd wvrrlglepg arkssdseig gsgtvsgsgd eltlhrstdc ivskelasss
      961 dsdllrsapp wtvahrnqkf rfspererer eraeklqyqq hqrdiqdhkq qaaekekesg
     1021 alsdssktis sqeeekptke dreakktrvk qslmkrarsv aifslklker rareaelkak
     1081 eaekearwqq pqscvggels cipieklisv ddiaamelrr lnh
//
LOCUS       XP_076545995            1121 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 8 white walker isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076545995
VERSION     XP_076545995.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689880.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1121
                     /product="anoctamin 8 white walker isoform X3"
                     /calculated_mol_wt=124875
     Region          269..751
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     Region          992..>1082
                     /region_name="CAF-1_p150"
                     /note="Chromatin assembly factor 1 complex p150 subunit,
                     N-terminal; pfam11600"
                     /db_xref="CDD:402959"
     CDS             1..1121
                     /gene="wwk"
                     /coded_by="XM_076689880.1:46..3411"
                     /db_xref="GeneID:117603311"
ORIGIN      
        1 mpggegspin lldhrtstts gecansdede rttatasstm ttvsggdglr rrkvihaake
       61 tldkasrllr rkipctghlm tprrlwiqkv ptqecdvvmm fpsgasdetl mwllgrlrag
      121 tpglvvhvrh hassdsysfy ltapfsvllk aaeevhlpkt lrqefggglk efvgtesscf
      181 egsddearff ttqerqslvl hllhtlragp qdlhslsglk mvegqaiipk cisagiisqv
      241 fplhelpale klqrtwvraf lspqplddic kyfgvkitmy fawlghytta livpaavgai
      301 ywvgiigrnq avedvayvlf svfnviwatv yletwkrrga elayrwgtld qrddllvepr
      361 plftgtleis hvtgrlepty prwkrnmfry fvsvpviaac lffvfivmil sfqiqdwwda
      421 rlesrgygfw lsyvpkvlla vvialmdeay fkvavwlndm enyrldteye nhliykvalf
      481 qfvnsflslf yiafylqdqe rlkeqlaall iarqvignlk esavpylieq lrlarlsfel
      541 fgalspsear sppgeenade nqkdaedkse rsesgkstqp rnvsqaeles slyrvghptn
      601 sllsdvtfkv stkkydgafs ehlemlsqlg yvclfssafp laamaallgn llelrgdafk
      661 lcfvlqrpfg rrvsnigtwq nameamglva ivvncaligl sgqvqrmfpe msatqtilli
      721 valehimlgi rfiiicaipd iphwvatema kveflrreav rrlsstpspe qqpatviadg
      781 eseeqqlltd rtgdattssl pdtptlastt htpsspptps aasvpriset psaaqtpgsa
      841 gssdigspfl tdngmgsvrd lhnsprcsip ggergrrsre wlatepeasg gsdhyshhlt
      901 igphggvdwv rrlglepgar kssdseiggs gtvsgsgdel tlhrstdciv skelasssds
      961 dllrsappwt vahrnqkfrf sperererer aeklqyqqhq rdiqdhkqqa aekekesgal
     1021 sdssktissq eeekptkedr eakktrvkqs lmkrarsvai fslklkerra reaelkakea
     1081 ekearwqqpq scvggelsci pieklisvdd iaamelrrln h
//
LOCUS       XP_076545996            1120 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 8 white walker isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076545996
VERSION     XP_076545996.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689881.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1120
                     /product="anoctamin 8 white walker isoform X4"
                     /calculated_mol_wt=124804
     Region          269..751
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     Region          991..>1081
                     /region_name="CAF-1_p150"
                     /note="Chromatin assembly factor 1 complex p150 subunit,
                     N-terminal; pfam11600"
                     /db_xref="CDD:402959"
     CDS             1..1120
                     /gene="wwk"
                     /coded_by="XM_076689881.1:46..3408"
                     /db_xref="GeneID:117603311"
ORIGIN      
        1 mpggegspin lldhrtstts gecansdede rttatasstm ttvsggdglr rrkvihaake
       61 tldkasrllr rkipctghlm tprrlwiqkv ptqecdvvmm fpsgasdetl mwllgrlrag
      121 tpglvvhvrh hassdsysfy ltapfsvllk aaeevhlpkt lrqefggglk efvgtesscf
      181 egsddearff ttqerqslvl hllhtlragp qdlhslsglk mvegqaiipk cisagiisqv
      241 fplhelpale klqrtwvraf lspqplddic kyfgvkitmy fawlghytta livpaavgai
      301 ywvgiigrnq avedvayvlf svfnviwatv yletwkrrga elayrwgtld qrddllvepr
      361 plftgtleis hvtgrlepty prwkrnmfry fvsvpviaac lffvfivmil sfqiqdwwda
      421 rlesrgygfw lsyvpkvlla vvialmdeay fkvavwlndm enyrldteye nhliykvalf
      481 qfvnsflslf yiafylqdqe rlkeqlaall iarqvignlk esavpylieq lrlarlsfel
      541 fgalspsear sppgeenade nqkdaedkse rsesgkstqp rnvsqaeles slyrvghptn
      601 sllsdvtfkv stkkydgafs ehlemlsqlg yvclfssafp laamaallgn llelrgdafk
      661 lcfvlqrpfg rrvsnigtwq nameamglva ivvncaligl sgqvqrmfpe msatqtilli
      721 valehimlgi rfiiicaipd iphwvatema kveflrreav rrlsstpspe qqpatvidge
      781 seeqqlltdr tgdattsslp dtptlastth tpsspptpsa asvprisetp saaqtpgsag
      841 ssdigspflt dngmgsvrdl hnsprcsipg gergrrsrew latepeasgg sdhyshhlti
      901 gphggvdwvr rlglepgark ssdseiggsg tvsgsgdelt lhrstdcivs kelasssdsd
      961 llrsappwtv ahrnqkfrfs perererera eklqyqqhqr diqdhkqqaa ekekesgals
     1021 dssktissqe eekptkedre akktrvkqsl mkrarsvaif slklkerrar eaelkakeae
     1081 kearwqqpqs cvggelscip ieklisvddi aamelrrlnh
//
LOCUS       XP_034178236            1108 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 8 white walker isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034178236
VERSION     XP_034178236.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322345.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1108
                     /product="anoctamin 8 white walker isoform X5"
                     /calculated_mol_wt=123477
     Region          269..731
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     Region          979..>1069
                     /region_name="CAF-1_p150"
                     /note="Chromatin assembly factor 1 complex p150 subunit,
                     N-terminal; pfam11600"
                     /db_xref="CDD:402959"
     CDS             1..1108
                     /gene="wwk"
                     /coded_by="XM_034322345.2:46..3372"
                     /db_xref="GeneID:117603311"
ORIGIN      
        1 mpggegspin lldhrtstts gecansdede rttatasstm ttvsggdglr rrkvihaake
       61 tldkasrllr rkipctghlm tprrlwiqkv ptqecdvvmm fpsgasdetl mwllgrlrag
      121 tpglvvhvrh hassdsysfy ltapfsvllk aaeevhlpkt lrqefggglk efvgtesscf
      181 egsddearff ttqerqslvl hllhtlragp qdlhslsglk mvegqaiipk cisagiisqv
      241 fplhelpale klqrtwvraf lspqplddic kyfgvkitmy fawlghytta livpaavgai
      301 ywvgiigrnq avedvayvlf svfnviwatv yletwkrrga elayrwgtld qrddllvepr
      361 plftgtleis hvtgrlepty prwkrnmfry fvsvpviaac lffvfivmil sfqiqdwwda
      421 rlesrgygfw lsyvpkvlla vvialmdeay fkvavwlndm enyrldteye nhliykvalf
      481 qfvnsflslf yiafylqdqe rlkeqlaall iarqvignlk esavpylieq lrlarlsfel
      541 fgalspsear sppgeenade nqkdaedkse rsesgkstqp rnvsqaeles slyrydgafs
      601 ehlemlsqlg yvclfssafp laamaallgn llelrgdafk lcfvlqrpfg rrvsnigtwq
      661 nameamglva ivvncaligl sgqvqrmfpe msatqtilli valehimlgi rfiiicaipd
      721 iphwvatema kveflrreav rrlsstpspe qqpatvigrf vvspadgese eqqlltdrtg
      781 dattsslpdt ptlastthtp sspptpsaas vprisetpsa aqtpgsagss digspfltdn
      841 gmgsvrdlhn sprcsipgge rgrrsrewla tepeasggsd hyshhltigp hggvdwvrrl
      901 glepgarkss dseiggsgtv sgsgdeltlh rstdcivske lasssdsdll rsappwtvah
      961 rnqkfrfspe rerereraek lqyqqhqrdi qdhkqqaaek ekesgalsds sktissqeee
     1021 kptkedreak ktrvkqslmk rarsvaifsl klkerrarea elkakeaeke arwqqpqscv
     1081 ggelscipie klisvddiaa melrrlnh
//
LOCUS       XP_034178246             594 aa            linear   INV 03-SEP-2025
DEFINITION  mau2 sister chromatid cohesion factor [Osmia lignaria lignaria].
ACCESSION   XP_034178246
VERSION     XP_034178246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..594
                     /product="mau2 sister chromatid cohesion factor"
                     /calculated_mol_wt=67238
     Region          8..538
                     /region_name="Cohesin_load"
                     /note="Cohesin loading factor; pfam10345"
                     /db_xref="CDD:463053"
     Region          48..77
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(49,52..53,56..57,59,91,94..95,98..99,101..102,125,
                     128..129,136..137,140)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          89..119
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          365..393
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(366,369..370,373..374,376,403,406..407,410..411,
                     413..414,434,437..438,443..444,447)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          398..428
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          433..470
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..594
                     /gene="Mau2"
                     /coded_by="XM_034322355.2:440..2224"
                     /db_xref="GeneID:117603325"
ORIGIN      
        1 massqdawyl sllglaenfr tsnppniksc iqclqavfnf kppprveart hlqlgnillt
       61 htknidlars hleqawrlsq nintfddvkf eaasvvaely eqqqqpnlsk pilrkaiels
      121 qhnvywhcrl ifqlaqihas ekdfvaassl lavgvdyshi snasytrvlf llsrcmllli
      181 dkkftevhpl lnsaghhven wqgsphqkey lkvyflvlqv chylmagqvk svkpclkqlq
      241 qsiqtimsps wpadevvtgs nigdmfiwmp kdhlyvlvyl vtvmhsmqag ymdkaqkytd
      301 kaltqieklk ivdnkpilsv fqlmllehiv mcrlvmgnks valaeisqac qlcrrqprll
      361 qghrpqlhal lglyamsmnc meaaeaqfta alrtsqerel wtfanlnlai vylrtkreae
      421 lgallerinp eslpshshsl raaayyvqgl qaffgaryne akrylretlk mansedlnrl
      481 tscslvllgh iflslgnsre smnmvtpamq laskipdvhv qlwatailkd lyricgdpnr
      541 eseayqmhct fsqtllkdhf qstqmgehsl iqwtdgpvpa lpsnpppsts qail
//
LOCUS       XP_034178262             229 aa            linear   INV 03-SEP-2025
DEFINITION  STING ER exit protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034178262
VERSION     XP_034178262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..229
                     /product="STING ER exit protein isoform X1"
                     /calculated_mol_wt=26224
     CDS             1..229
                     /gene="LOC117603335"
                     /coded_by="XM_034322371.2:143..832"
                     /db_xref="GeneID:117603335"
ORIGIN      
        1 mpkvvsrsii csdtkdqeey seekplhiyy clcgqmtlil dcaieklplr krdgarvidg
       61 skhahkmtce qdevvylkrt egiekqyrqk ckkcinynly mylhrcglfl yykhdqgtnv
      121 vfivkgavik ssgegpmtdi ynqvaiekpk kimvtkhtkn mgkfssvtvs tideeedeie
      181 arevadsyan nariiekqle rkgmnkrkam pppeadpkra rprgtlldv
//
LOCUS       XP_034178271             217 aa            linear   INV 03-SEP-2025
DEFINITION  STING ER exit protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034178271
VERSION     XP_034178271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322380.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="STING ER exit protein isoform X2"
                     /calculated_mol_wt=24640
     CDS             1..217
                     /gene="LOC117603335"
                     /coded_by="XM_034322380.2:143..796"
                     /db_xref="GeneID:117603335"
ORIGIN      
        1 mpkvvsrsii csdtkdqeey seekplhiyy clcgqmtlil dcaieklplr krdgarvidg
       61 skhahkmtce qdevvylkrt egiekqyrqk ckkcglflyy khdqgtnvvf ivkgavikss
      121 gegpmtdiyn qvaiekpkki mvtkhtknmg kfssvtvsti deeedeiear evadsyanna
      181 riiekqlerk gmnkrkampp peadpkrarp rgtlldv
//
LOCUS       XP_034178284             224 aa            linear   INV 03-SEP-2025
DEFINITION  NADH-quinone oxidoreductase subunit B 2 [Osmia lignaria lignaria].
ACCESSION   XP_034178284
VERSION     XP_034178284.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322393.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..224
                     /product="NADH-quinone oxidoreductase subunit B 2"
                     /calculated_mol_wt=24805
     Region          56..224
                     /region_name="PRK06411"
                     /note="NADH-quinone oxidoreductase subunit NuoB"
                     /db_xref="CDD:235797"
     CDS             1..224
                     /gene="LOC117603349"
                     /coded_by="XM_034322393.2:136..810"
                     /db_xref="GeneID:117603349"
ORIGIN      
        1 mfrsimfspt inqglaslir nnialsgspg slvcqakqit tdvaaqqnqv svpgkvekqp
       61 yspfqntsnm aeyavarldd llnwgrkgsi wpltfglacc avemmhiaap rydmdrygvv
      121 frasprqadv iivagtltnk mapalrriyd qmpeprwvis mgscangggy yhysysvvrg
      181 cdriipvdiy vpgcpptaea lmygilqlqk kvkrmqtvqa wyrk
//
LOCUS       XP_034174281            1921 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174281
VERSION     XP_034174281.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318390.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1921
                     /product="TATA-box binding protein associated factor 1
                     isoform X1"
                     /calculated_mol_wt=219513
     Region          4..60
                     /region_name="TBP-binding"
                     /note="TATA box-binding protein binding; pfam09247"
                     /db_xref="CDD:462723"
     Region          592..1057
                     /region_name="DUF3591"
                     /note="Protein of unknown function (DUF3591); pfam12157"
                     /db_xref="CDD:463476"
     Region          1347..1379
                     /region_name="zf-CCHC_6"
                     /note="Zinc knuckle; pfam15288"
                     /db_xref="CDD:464616"
     Region          1467..1578
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1491,1496,1499,1538,1542,1548)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     Region          1589..1700
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1614,1619,1622,1661,1665,1671)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     CDS             1..1921
                     /gene="Taf1"
                     /coded_by="XM_034318390.2:290..6055"
                     /db_xref="GeneID:117601535"
ORIGIN      
        1 madseeendk dimsginmtg flfgniddng qleddildpe akqhlaslnr lglssfiqem
       61 mfndnsiegq kesndksked ieaidekein yiekspnald fsdineladd ereesnkdtf
      121 gekaekenad ydaddeevvn ksdtqlmppp pipeekealt aeeaeaarqr kletplasml
      181 pskyanvdvt elfpdfrank vlrfsrlfgp gkpsslpqiw rgvkkrrkkk rhhdirdsds
      241 gsdqdekkpk fkgwimqygt dptpdmcctd denkllmpve dkqhvgktge tgengdmgpk
      301 vadwrfgpaq lwydmlqvpe tgdgfnygfk llekpeesnn kdtkdirdtt dtldttdimd
      361 iqdtkdikds kdnseefsdd aflmvsqlhw eddviwngdd ikhkvlqkln sknnaagwvp
      421 ssgnrtaqaf sqpgkgapvp vasnvrlats qittplhmqs qknkmnmnkg nqqqareeny
      481 ddtwysifpv eneelvyglw eeeviwdpen mnkipkpkil tldpndeniv lgipddidpa
      541 lhhkdngpqp kvkiphphvk ksklllgkag vinvleedtp ppppkspdrd pfnisndtyy
      601 mprssettlr lkvgggnliq hstpvvelra pfvqthmgqm rlrnfhrppl rrfshgplah
      661 pgphsvlplv khikkkskqr eqeriasggg dvffmrtaed ltgkdgelvl vefseehppl
      721 mnqvgmcskv knyykrkagk dqgpqkykyg etayahtspf lgiltpgqsi qavennmyra
      781 piyehkipet dfliirtrqq yyirevdaif vagqecplye vpgpnskran nfvrdflqvf
      841 iyrlfwksrd tprrirmddi kkafpshses sirkrlklca dfkrtgmdsn wwvikpdfrl
      901 pteeeiramv speqccsyfs miaaeqrlkd agygekflft pqddddeemq lkmddevkva
      961 pwnttrayiq amkgkcllql agpadptgcg egfsyvrvpn kptiskeeqe aqpkrtvtgt
     1021 dadlrrlsln nakallrkfg vpeeeikkls rwevidvvrt lstekakage egmtkfsrgn
     1081 rfsiaehqer ykeecqrifd lqnrvlssne vlstdegess eedssdieem gknienmlsn
     1141 kktstqlsle reeqqrhelr kmlmgevheq dkkskekkkd deedspvnny naqqgrvlki
     1201 yrtfrnpegk eftrielvrk pavidtyiki rnskdetfik qfatldeaqk eemkrekrri
     1261 qeqlrrikrn qerermlggp mtgnnissnn ifdrscnntp tttssnsilp fcnsfqsisp
     1321 askhpkpets pskrkkpklk pdlklkcgac gnvghmrtnk acplyqnsit tapvnvamte
     1381 eqeeeiekql ntddqdlvnv dgtkvklssk likhaeemkr rtlllkvpke avnskkrrra
     1441 tgddhcdylk rqqrpanrrr tdpvvvmstm lesilnemrd lpdvqpflfp vnakavpdyy
     1501 kiiqrpmdlq tirenlrlkk yqsreeflad vnqivenstl ynglkssltv aakrmletcv
     1561 erlgekedrl mrlekainpl lddndqvalt fildnvvnnk lksmaeawpf lkpvnkklvk
     1621 dyytvikrpm dletiskkvs ahkyhsrhef vrdieqilen ctiyngkesp ftqkaelvvk
     1681 icketldeyd ehltqlenni llvqkrameq edydpswlgp deenytivep efrgsqtssp
     1741 enpfgksnvd dfdfvdvegd tegdgsrsan skkkdvleed lqfssedefd evpfgtdeqs
     1801 enaemeplel nevreggvvl adddsqqaae amvqlgnvgf ymadqqllqq desmdvdpny
     1861 dpsdfllagl pareekgenk iqddlavses dddaessaqq kqktpqqisq pdddvggdlw
     1921 f
//
LOCUS       XP_034174287            1918 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174287
VERSION     XP_034174287.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318396.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1918
                     /product="TATA-box binding protein associated factor 1
                     isoform X2"
                     /calculated_mol_wt=219173
     Region          4..60
                     /region_name="TBP-binding"
                     /note="TATA box-binding protein binding; pfam09247"
                     /db_xref="CDD:462723"
     Region          589..1054
                     /region_name="DUF3591"
                     /note="Protein of unknown function (DUF3591); pfam12157"
                     /db_xref="CDD:463476"
     Region          1344..1376
                     /region_name="zf-CCHC_6"
                     /note="Zinc knuckle; pfam15288"
                     /db_xref="CDD:464616"
     Region          1464..1575
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1488,1493,1496,1535,1539,1545)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     Region          1586..1697
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1611,1616,1619,1658,1662,1668)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     CDS             1..1918
                     /gene="Taf1"
                     /coded_by="XM_034318396.2:290..6046"
                     /db_xref="GeneID:117601535"
ORIGIN      
        1 madseeendk dimsginmtg flfgniddng qleddildpe akqhlaslnr lglssfiqem
       61 mfndnsiegq kesndksked ieaidekein yiekspnald fsdineladd ereesnkdtf
      121 gekaekenad ydaddeevvn ksdtqlmppp pipeekealt aeeaeaarqr kletplasml
      181 pskyanvdvt elfpdfrank vlrfsrlfgp gkpsslpqiw rgvkkrrkkk rhhdirdsds
      241 gsdqdekkpk fkgwimqygt dptpdmcctd denkllmpve dkqhvgktge tgengdmgpk
      301 vadwrfgpaq lwydmlqvpe tgdgfnygfk llekpeesnn kdtkdirdtt dtldttdimd
      361 iqdtkdikds kdnseefsdd aflmvsqlhw eddviwngdd ikhkklnskn naagwvpssg
      421 nrtaqafsqp gkgapvpvas nvrlatsqit tplhmqsqkn kmnmnkgnqq qareenyddt
      481 wysifpvene elvyglweee viwdpenmnk ipkpkiltld pndenivlgi pddidpalhh
      541 kdngpqpkvk iphphvkksk lllgkagvin vleedtpppp pkspdrdpfn isndtyympr
      601 ssettlrlkv gggnliqhst pvvelrapfv qthmgqmrlr nfhrpplrrf shgplahpgp
      661 hsvlplvkhi kkkskqreqe riasgggdvf fmrtaedltg kdgelvlvef seehpplmnq
      721 vgmcskvkny ykrkagkdqg pqkykygeta yahtspflgi ltpgqsiqav ennmyrapiy
      781 ehkipetdfl iirtrqqyyi revdaifvag qecplyevpg pnskrannfv rdflqvfiyr
      841 lfwksrdtpr rirmddikka fpshsessir krlklcadfk rtgmdsnwwv ikpdfrlpte
      901 eeiramvspe qccsyfsmia aeqrlkdagy gekflftpqd dddeemqlkm ddevkvapwn
      961 ttrayiqamk gkcllqlagp adptgcgegf syvrvpnkpt iskeeqeaqp krtvtgtdad
     1021 lrrlslnnak allrkfgvpe eeikklsrwe vidvvrtlst ekakageegm tkfsrgnrfs
     1081 iaehqeryke ecqrifdlqn rvlssnevls tdegesseed ssdieemgkn ienmlsnkkt
     1141 stqlsleree qqrhelrkml mgevheqdkk skekkkddee dspvnnynaq qgrvlkiyrt
     1201 frnpegkeft rielvrkpav idtyikirns kdetfikqfa tldeaqkeem krekrriqeq
     1261 lrrikrnqer ermlggpmtg nnissnnifd rscnntpttt ssnsilpfcn sfqsispask
     1321 hpkpetspsk rkkpklkpdl klkcgacgnv ghmrtnkacp lyqnsittap vnvamteeqe
     1381 eeiekqlntd dqdlvnvdgt kvklssklik haeemkrrtl llkvpkeavn skkrrratgd
     1441 dhcdylkrqq rpanrrrtdp vvvmstmles ilnemrdlpd vqpflfpvna kavpdyykii
     1501 qrpmdlqtir enlrlkkyqs reefladvnq ivenstlyng lkssltvaak rmletcverl
     1561 gekedrlmrl ekainplldd ndqvaltfil dnvvnnklks maeawpflkp vnkklvkdyy
     1621 tvikrpmdle tiskkvsahk yhsrhefvrd ieqilencti yngkespftq kaelvvkick
     1681 etldeydehl tqlennillv qkrameqedy dpswlgpdee nytivepefr gsqtsspenp
     1741 fgksnvddfd fvdvegdteg dgsrsanskk kdvleedlqf ssedefdevp fgtdeqsena
     1801 emeplelnev reggvvladd dsqqaaeamv qlgnvgfyma dqqllqqdes mdvdpnydps
     1861 dfllaglpar eekgenkiqd dlavsesddd aessaqqkqk tpqqisqpdd dvggdlwf
//
LOCUS       XP_034174297            1914 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 1 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174297
VERSION     XP_034174297.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318406.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1914
                     /product="TATA-box binding protein associated factor 1
                     isoform X3"
                     /calculated_mol_wt=218699
     Region          4..60
                     /region_name="TBP-binding"
                     /note="TATA box-binding protein binding; pfam09247"
                     /db_xref="CDD:462723"
     Region          592..1057
                     /region_name="DUF3591"
                     /note="Protein of unknown function (DUF3591); pfam12157"
                     /db_xref="CDD:463476"
     Region          1347..1379
                     /region_name="zf-CCHC_6"
                     /note="Zinc knuckle; pfam15288"
                     /db_xref="CDD:464616"
     Region          1467..1578
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1491,1496,1499,1538,1542,1548)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     Region          1589..1700
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1614,1619,1622,1661,1665,1671)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     CDS             1..1914
                     /gene="Taf1"
                     /coded_by="XM_034318406.2:290..6034"
                     /db_xref="GeneID:117601535"
ORIGIN      
        1 madseeendk dimsginmtg flfgniddng qleddildpe akqhlaslnr lglssfiqem
       61 mfndnsiegq kesndksked ieaidekein yiekspnald fsdineladd ereesnkdtf
      121 gekaekenad ydaddeevvn ksdtqlmppp pipeekealt aeeaeaarqr kletplasml
      181 pskyanvdvt elfpdfrank vlrfsrlfgp gkpsslpqiw rgvkkrrkkk rhhdirdsds
      241 gsdqdekkpk fkgwimqygt dptpdmcctd denkllmpve dkqhvgktge tgengdmgpk
      301 vadwrfgpaq lwydmlqvpe tgdgfnygfk llekpeesnn kdtkdirdtt dtldttdimd
      361 iqdtkdikds kdnseefsdd aflmvsqlhw eddviwngdd ikhkvlqkln sknnaagwvp
      421 ssgnrtaqaf sqpgkgapvp vasnvrlats qittplhmqs qknkmnmnkg nqqqareeny
      481 ddtwysifpv eneelvyglw eeeviwdpen mnkipkpkil tldpndeniv lgipddidpa
      541 lhhkdngpqp kvkiphphvk ksklllgkag vinvleedtp ppppkspdrd pfnisndtyy
      601 mprssettlr lkvgggnliq hstpvvelra pfvqthmgqm rlrnfhrppl rrfshgplah
      661 pgphsvlplv khikkkskqr eqeriasggg dvffmrtaed ltgkdgelvl vefseehppl
      721 mnqvgmcskv knyykrkagk dqgpqkykyg etayahtspf lgiltpgqsi qavennmyra
      781 piyehkipet dfliirtrqq yyirevdaif vagqecplye vpgpnskran nfvrdflqvf
      841 iyrlfwksrd tprrirmddi kkafpshses sirkrlklca dfkrtgmdsn wwvikpdfrl
      901 pteeeiramv speqccsyfs miaaeqrlkd agygekflft pqddddeemq lkmddevkva
      961 pwnttrayiq amkgkcllql agpadptgcg egfsyvrvpn kptiskeeqe aqpkrtvtgt
     1021 dadlrrlsln nakallrkfg vpeeeikkls rwevidvvrt lstekakage egmtkfsrgn
     1081 rfsiaehqer ykeecqrifd lqnrvlssne vlstdegess eedssdieem gknienmlsn
     1141 kktstqlsle reeqqrhelr kmlmgevheq dkkskekkkd deedspvnny naqqgrvlki
     1201 yrtfrnpegk eftrielvrk pavidtyiki rnskdetfik qfatldeaqk eemkrekrri
     1261 qeqlrrikrn qerermlggp mtgnnissnn ifdrscnntp tttssnsilp fcnsfqsisp
     1321 askhpkpets pskrkkpklk pdlklkcgac gnvghmrtnk acplyqnsit tapvnvamte
     1381 eqeeeiekql ntddqdlvnv dgtkvklssk likhaeemkr rtlllkvpke avnskkrrra
     1441 tgddhcdylk rqqrpanrrr tdpvvvmstm lesilnemrd lpdvqpflfp vnakavpdyy
     1501 kiiqrpmdlq tirenlrlkk yqsreeflad vnqivenstl ynglkssltv aakrmletcv
     1561 erlgekedrl mrlekainpl lddndqvalt fildnvvnnk lksmaeawpf lkpvnkklvk
     1621 dyytvikrpm dletiskkvs ahkyhsrhef vrdieqilen ctiyngkesp ftqkaelvvk
     1681 icketldeyd ehltqlenni llvqkrameq edydpswlgp deenytisqt sspenpfgks
     1741 nvddfdfvdv egdtegdgsr sanskkkdvl eedlqfssed efdevpfgtd eqsenaemep
     1801 lelnevregg vvladddsqq aaeamvqlgn vgfymadqql lqqdesmdvd pnydpsdfll
     1861 aglpareekg enkiqddlav sesdddaess aqqkqktpqq isqpdddvgg dlwf
//
LOCUS       XP_034174306            1906 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 1 isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174306
VERSION     XP_034174306.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318415.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1906
                     /product="TATA-box binding protein associated factor 1
                     isoform X4"
                     /calculated_mol_wt=217819
     Region          4..60
                     /region_name="TBP-binding"
                     /note="TATA box-binding protein binding; pfam09247"
                     /db_xref="CDD:462723"
     Region          592..1057
                     /region_name="DUF3591"
                     /note="Protein of unknown function (DUF3591); pfam12157"
                     /db_xref="CDD:463476"
     Region          1347..1379
                     /region_name="zf-CCHC_6"
                     /note="Zinc knuckle; pfam15288"
                     /db_xref="CDD:464616"
     Region          1467..1578
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1491,1496,1499,1538,1542,1548)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     Region          1589..1700
                     /region_name="Bromo_TFIID"
                     /note="Bromodomain, TFIID-like subfamily. Human TAFII250
                     (or TAF250) is the largest subunit of TFIID, a large
                     multi-domain complex, which initiates the assembly of the
                     transcription machinery. TAFII250 contains two
                     bromodomains that specifically bind to...; cd05511"
                     /db_xref="CDD:99943"
     Site            order(1614,1619,1622,1661,1665,1671)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99943"
     CDS             1..1906
                     /gene="Taf1"
                     /coded_by="XM_034318415.2:290..6010"
                     /db_xref="GeneID:117601535"
ORIGIN      
        1 madseeendk dimsginmtg flfgniddng qleddildpe akqhlaslnr lglssfiqem
       61 mfndnsiegq kesndksked ieaidekein yiekspnald fsdineladd ereesnkdtf
      121 gekaekenad ydaddeevvn ksdtqlmppp pipeekealt aeeaeaarqr kletplasml
      181 pskyanvdvt elfpdfrank vlrfsrlfgp gkpsslpqiw rgvkkrrkkk rhhdirdsds
      241 gsdqdekkpk fkgwimqygt dptpdmcctd denkllmpve dkqhvgktge tgengdmgpk
      301 vadwrfgpaq lwydmlqvpe tgdgfnygfk llekpeesnn kdtkdirdtt dtldttdimd
      361 iqdtkdikds kdnseefsdd aflmvsqlhw eddviwngdd ikhkvlqkln sknnaagwvp
      421 ssgnrtaqaf sqpgkgapvp vasnvrlats qittplhmqs qknkmnmnkg nqqqareeny
      481 ddtwysifpv eneelvyglw eeeviwdpen mnkipkpkil tldpndeniv lgipddidpa
      541 lhhkdngpqp kvkiphphvk ksklllgkag vinvleedtp ppppkspdrd pfnisndtyy
      601 mprssettlr lkvgggnliq hstpvvelra pfvqthmgqm rlrnfhrppl rrfshgplah
      661 pgphsvlplv khikkkskqr eqeriasggg dvffmrtaed ltgkdgelvl vefseehppl
      721 mnqvgmcskv knyykrkagk dqgpqkykyg etayahtspf lgiltpgqsi qavennmyra
      781 piyehkipet dfliirtrqq yyirevdaif vagqecplye vpgpnskran nfvrdflqvf
      841 iyrlfwksrd tprrirmddi kkafpshses sirkrlklca dfkrtgmdsn wwvikpdfrl
      901 pteeeiramv speqccsyfs miaaeqrlkd agygekflft pqddddeemq lkmddevkva
      961 pwnttrayiq amkgkcllql agpadptgcg egfsyvrvpn kptiskeeqe aqpkrtvtgt
     1021 dadlrrlsln nakallrkfg vpeeeikkls rwevidvvrt lstekakage egmtkfsrgn
     1081 rfsiaehqer ykeecqrifd lqnrvlssne vlstdegess eedssdieem gknienmlsn
     1141 kktstqlsle reeqqrhelr kmlmgevheq dkkskekkkd deedspvnny naqqgrvlki
     1201 yrtfrnpegk eftrielvrk pavidtyiki rnskdetfik qfatldeaqk eemkrekrri
     1261 qeqlrrikrn qerermlggp mtgnnissnn ifdrscnntp tttssnsilp fcnsfqsisp
     1321 askhpkpets pskrkkpklk pdlklkcgac gnvghmrtnk acplyqnsit tapvnvamte
     1381 eqeeeiekql ntddqdlvnv dgtkvklssk likhaeemkr rtlllkvpke avnskkrrra
     1441 tgddhcdylk rqqrpanrrr tdpvvvmstm lesilnemrd lpdvqpflfp vnakavpdyy
     1501 kiiqrpmdlq tirenlrlkk yqsreeflad vnqivenstl ynglkssltv aakrmletcv
     1561 erlgekedrl mrlekainpl lddndqvalt fildnvvnnk lksmaeawpf lkpvnkklvk
     1621 dyytvikrpm dletiskkvs ahkyhsrhef vrdieqilen ctiyngkesp ftqkaelvvk
     1681 icketldeyd ehltqlenni llvqkrameq edydpswlgp deenytivep efrgsqtssp
     1741 enpfgksnvd dfdfvdvegd tegdgsrsan skkkdvleed lqfssedefd evpfgtdeqs
     1801 enaemeplel nevreggvvl adddsqqaae amvqldesmd vdpnydpsdf llaglparee
     1861 kgenkiqddl avsesdddae ssaqqkqktp qqisqpdddv ggdlwf
//
LOCUS       XP_076545997             909 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305634 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545997
VERSION     XP_076545997.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689882.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..909
                     /product="uncharacterized protein LOC143305634 isoform X1"
                     /calculated_mol_wt=105419
     Region          522..605
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <836..894
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          843..864
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          872..888
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..909
                     /gene="LOC143305634"
                     /coded_by="XM_076689882.1:73..2802"
                     /db_xref="GeneID:143305634"
ORIGIN      
        1 mitdadlktk mgyikeicsi pthslrdqdk ilsniihvtr nalqdqlliy npdniilctl
       61 klffnykenv svtdnvcewk rrkkrrlake cynsllqvyv pekskeilea iismiyedkl
      121 wefesfcfei mcdlleygis gallihaiwd rievaninme dtrkiirily elldvydwpd
      181 nydtvmvier ilnlfyisit srqyspnsya ilkkglevcl inivkhvvnd hlliivqymc
      241 swavekgmtn eiilefgstl eytaymhevt lfektltpkv fpllmqmias kskitsllgn
      301 rviqylldrq dnrmnfdtpk iffedtqfdl kigkclkedk lfcklhreil hdsllksiin
      361 hctsrmnlet tycticliat evpcgftaaa lvclimnlqd ltlkeskrnt evsyhlhatv
      421 isimsllcwi hkakvfyeyv nkimmeraqw aphlnppiqs qysfaahhil wnkpelffvd
      481 wearyliisr eimsgeqfsl vwnsfprnls sglytlltde qlvdvtlaae gqilrahkli
      541 lsvcstyfre lfkensckhp ivilkdvnyr dlsamlhfmy qgevnikqed iasflkvaet
      601 lqikglttet eekleealsk naqnldkifn aeqnnsvrsd lnihsfekak qnqqsqgqei
      661 sesnfiekds nvnnvccqqh ctsdssyniq dihmtrsdcs rgiepecdee epldctadis
      721 hvqskhepld ytldidaetg yktylpnepf hkpeenvhrk dfvpdmqlvp ysqnvqsqsf
      781 glnnmtgleg efpyelgchn kgrrtvkgis nnslplettl rvvselgptl rmergkvirm
      841 yscpwclrhf trkenlklhv ryihgplesl tcefcgnkyk nsnslrvhsy lyhnpkrdkh
      901 skplvdgga
//
LOCUS       XP_076545998             644 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305634 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545998
VERSION     XP_076545998.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689883.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..644
                     /product="uncharacterized protein LOC143305634 isoform X2"
                     /calculated_mol_wt=74227
     Region          257..340
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <571..629
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          578..599
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          607..623
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..644
                     /gene="LOC143305634"
                     /coded_by="XM_076689883.1:176..2110"
                     /db_xref="GeneID:143305634"
ORIGIN      
        1 mhevtlfekt ltpkvfpllm qmiaskskit sllgnrviqy lldrqdnrmn fdtpkiffed
       61 tqfdlkigkc lkedklfckl hreilhdsll ksiinhctsr mnlettycti cliatevpcg
      121 ftaaalvcli mnlqdltlke skrntevsyh lhatvisims llcwihkakv fyeyvnkimm
      181 eraqwaphln ppiqsqysfa ahhilwnkpe lffvdweary liisreimsg eqfslvwnsf
      241 prnlssglyt lltdeqlvdv tlaaegqilr ahklilsvcs tyfrelfken sckhpivilk
      301 dvnyrdlsam lhfmyqgevn ikqediasfl kvaetlqikg ltteteekle ealsknaqnl
      361 dkifnaeqnn svrsdlnihs fekakqnqqs qgqeisesnf iekdsnvnnv ccqqhctsds
      421 syniqdihmt rsdcsrgiep ecdeeepldc tadishvqsk hepldytldi daetgyktyl
      481 pnepfhkpee nvhrkdfvpd mqlvpysqnv qsqsfglnnm tglegefpye lgchnkgrrt
      541 vkgisnnslp lettlrvvse lgptlrmerg kvirmyscpw clrhftrken lklhvryihg
      601 plesltcefc gnkyknsnsl rvhsylyhnp krdkhskplv dgga
//
LOCUS       XP_076545999             596 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305634 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076545999
VERSION     XP_076545999.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689884.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="uncharacterized protein LOC143305634 isoform X3"
                     /calculated_mol_wt=68627
     Region          209..292
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <523..581
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          530..551
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          559..575
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..596
                     /gene="LOC143305634"
                     /coded_by="XM_076689884.1:60..1850"
                     /db_xref="GeneID:143305634"
ORIGIN      
        1 mnfdtpkiff edtqfdlkig kclkedklfc klhreilhds llksiinhct srmnlettyc
       61 ticliatevp cgftaaalvc limnlqdltl keskrntevs yhlhatvisi msllcwihka
      121 kvfyeyvnki mmeraqwaph lnppiqsqys faahhilwnk pelffvdwea ryliisreim
      181 sgeqfslvwn sfprnlssgl ytlltdeqlv dvtlaaegqi lrahklilsv cstyfrelfk
      241 ensckhpivi lkdvnyrdls amlhfmyqge vnikqedias flkvaetlqi kgltteteek
      301 leealsknaq nldkifnaeq nnsvrsdlni hsfekakqnq qsqgqeises nfiekdsnvn
      361 nvccqqhcts dssyniqdih mtrsdcsrgi epecdeeepl dctadishvq skhepldytl
      421 didaetgykt ylpnepfhkp eenvhrkdfv pdmqlvpysq nvqsqsfgln nmtglegefp
      481 yelgchnkgr rtvkgisnns lplettlrvv selgptlrme rgkvirmysc pwclrhftrk
      541 enlklhvryi hgplesltce fcgnkyknsn slrvhsylyh npkrdkhskp lvdgga
//
LOCUS       XP_076546000             519 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546000
VERSION     XP_076546000.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689885.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="tyrosine-protein kinase Src42A isoform X1"
                     /calculated_mol_wt=59490
     Region          72..123
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(77,79,82..83,86,103..105,118,120,122..123)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          131..226
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(142,162,164..167,172,183,185,218)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(142,162,183,185)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(184,212)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          244..509
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(259,261..263,267,279,281,325..328,332,372,376..377,
                     379,390,407..411,420,454)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(259,262,267,279,281,325..328,332,372,374,377,379,
                     390)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(273,275,307,310,312,326,351,355,383,503)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(361,364..365,428,490..492,494,496..497,500,504,
                     507..508)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(372,376,407..411,420,454)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(389..398,399..413)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..519
                     /gene="Src42A"
                     /coded_by="XM_076689885.1:119..1678"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mhsktfrngi lqrffsryfs sptdtekekp drignpniea vasqaspvpt pppdpirpmp
       61 qipdadvpta kifvalydyd artdedlsfr kgehleilnd tqgdwwlars krtrqegyip
      121 snyvaklksi eaepwyfrki krieaekkll lpendhgafl irdsesrhnd yslsvrdgdt
      181 vkhyrirqld eggffiarrt tfrnlqdlve hyskdadglc vnlckpcvqv ekpvteglsh
      241 rtrdqweidr sslkfvrklg qgqfgevweg swnnttavai ktlkpgtmdp kdflaeaqim
      301 kklrhakliq lyavctleep iyiitelmrn gslleylqgk gkslnlqrli dmaaqiaagm
      361 aylesqnyih rdlaarnvlv aelnvvkiad fglarliked eyearvgarf pikwtapeaa
      421 nyskfsiksd vwsfgillte lvtygripyp gmtnaevlhq vehgyrmpcp pgcptalydi
      481 mlecwnkdpm krptfetlqw kledfftmeg seykeasay
//
LOCUS       XP_076546001             519 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546001
VERSION     XP_076546001.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689886.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="tyrosine-protein kinase Src42A isoform X1"
                     /calculated_mol_wt=59490
     Region          72..123
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(77,79,82..83,86,103..105,118,120,122..123)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          131..226
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(142,162,164..167,172,183,185,218)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(142,162,183,185)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(184,212)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          244..509
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(259,261..263,267,279,281,325..328,332,372,376..377,
                     379,390,407..411,420,454)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(259,262,267,279,281,325..328,332,372,374,377,379,
                     390)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(273,275,307,310,312,326,351,355,383,503)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(361,364..365,428,490..492,494,496..497,500,504,
                     507..508)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(372,376,407..411,420,454)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(389..398,399..413)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..519
                     /gene="Src42A"
                     /coded_by="XM_076689886.1:119..1678"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mhsktfrngi lqrffsryfs sptdtekekp drignpniea vasqaspvpt pppdpirpmp
       61 qipdadvpta kifvalydyd artdedlsfr kgehleilnd tqgdwwlars krtrqegyip
      121 snyvaklksi eaepwyfrki krieaekkll lpendhgafl irdsesrhnd yslsvrdgdt
      181 vkhyrirqld eggffiarrt tfrnlqdlve hyskdadglc vnlckpcvqv ekpvteglsh
      241 rtrdqweidr sslkfvrklg qgqfgevweg swnnttavai ktlkpgtmdp kdflaeaqim
      301 kklrhakliq lyavctleep iyiitelmrn gslleylqgk gkslnlqrli dmaaqiaagm
      361 aylesqnyih rdlaarnvlv aelnvvkiad fglarliked eyearvgarf pikwtapeaa
      421 nyskfsiksd vwsfgillte lvtygripyp gmtnaevlhq vehgyrmpcp pgcptalydi
      481 mlecwnkdpm krptfetlqw kledfftmeg seykeasay
//
LOCUS       XP_076546002             519 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546002
VERSION     XP_076546002.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689887.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="tyrosine-protein kinase Src42A isoform X1"
                     /calculated_mol_wt=59490
     Region          72..123
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(77,79,82..83,86,103..105,118,120,122..123)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          131..226
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(142,162,164..167,172,183,185,218)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(142,162,183,185)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(184,212)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          244..509
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(259,261..263,267,279,281,325..328,332,372,376..377,
                     379,390,407..411,420,454)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(259,262,267,279,281,325..328,332,372,374,377,379,
                     390)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(273,275,307,310,312,326,351,355,383,503)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(361,364..365,428,490..492,494,496..497,500,504,
                     507..508)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(372,376,407..411,420,454)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(389..398,399..413)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..519
                     /gene="Src42A"
                     /coded_by="XM_076689887.1:119..1678"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mhsktfrngi lqrffsryfs sptdtekekp drignpniea vasqaspvpt pppdpirpmp
       61 qipdadvpta kifvalydyd artdedlsfr kgehleilnd tqgdwwlars krtrqegyip
      121 snyvaklksi eaepwyfrki krieaekkll lpendhgafl irdsesrhnd yslsvrdgdt
      181 vkhyrirqld eggffiarrt tfrnlqdlve hyskdadglc vnlckpcvqv ekpvteglsh
      241 rtrdqweidr sslkfvrklg qgqfgevweg swnnttavai ktlkpgtmdp kdflaeaqim
      301 kklrhakliq lyavctleep iyiitelmrn gslleylqgk gkslnlqrli dmaaqiaagm
      361 aylesqnyih rdlaarnvlv aelnvvkiad fglarliked eyearvgarf pikwtapeaa
      421 nyskfsiksd vwsfgillte lvtygripyp gmtnaevlhq vehgyrmpcp pgcptalydi
      481 mlecwnkdpm krptfetlqw kledfftmeg seykeasay
//
LOCUS       XP_034190850             519 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190850
VERSION     XP_034190850.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334959.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190850.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="tyrosine-protein kinase Src42A isoform X1"
                     /calculated_mol_wt=59490
     Region          72..123
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(77,79,82..83,86,103..105,118,120,122..123)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          131..226
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(142,162,164..167,172,183,185,218)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(142,162,183,185)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(184,212)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          244..509
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(259,261..263,267,279,281,325..328,332,372,376..377,
                     379,390,407..411,420,454)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(259,262,267,279,281,325..328,332,372,374,377,379,
                     390)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(273,275,307,310,312,326,351,355,383,503)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(361,364..365,428,490..492,494,496..497,500,504,
                     507..508)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(372,376,407..411,420,454)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(389..398,399..413)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..519
                     /gene="Src42A"
                     /coded_by="XM_034334959.2:119..1678"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mhsktfrngi lqrffsryfs sptdtekekp drignpniea vasqaspvpt pppdpirpmp
       61 qipdadvpta kifvalydyd artdedlsfr kgehleilnd tqgdwwlars krtrqegyip
      121 snyvaklksi eaepwyfrki krieaekkll lpendhgafl irdsesrhnd yslsvrdgdt
      181 vkhyrirqld eggffiarrt tfrnlqdlve hyskdadglc vnlckpcvqv ekpvteglsh
      241 rtrdqweidr sslkfvrklg qgqfgevweg swnnttavai ktlkpgtmdp kdflaeaqim
      301 kklrhakliq lyavctleep iyiitelmrn gslleylqgk gkslnlqrli dmaaqiaagm
      361 aylesqnyih rdlaarnvlv aelnvvkiad fglarliked eyearvgarf pikwtapeaa
      421 nyskfsiksd vwsfgillte lvtygripyp gmtnaevlhq vehgyrmpcp pgcptalydi
      481 mlecwnkdpm krptfetlqw kledfftmeg seykeasay
//
LOCUS       XP_034191019             519 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191019
VERSION     XP_034191019.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335128.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191019.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="tyrosine-protein kinase Src42A isoform X1"
                     /calculated_mol_wt=59490
     Region          72..123
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(77,79,82..83,86,103..105,118,120,122..123)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          131..226
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(142,162,164..167,172,183,185,218)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(142,162,183,185)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(184,212)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          244..509
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(259,261..263,267,279,281,325..328,332,372,376..377,
                     379,390,407..411,420,454)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(259,262,267,279,281,325..328,332,372,374,377,379,
                     390)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(273,275,307,310,312,326,351,355,383,503)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(361,364..365,428,490..492,494,496..497,500,504,
                     507..508)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(372,376,407..411,420,454)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(389..398,399..413)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..519
                     /gene="Src42A"
                     /coded_by="XM_034335128.2:119..1678"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mhsktfrngi lqrffsryfs sptdtekekp drignpniea vasqaspvpt pppdpirpmp
       61 qipdadvpta kifvalydyd artdedlsfr kgehleilnd tqgdwwlars krtrqegyip
      121 snyvaklksi eaepwyfrki krieaekkll lpendhgafl irdsesrhnd yslsvrdgdt
      181 vkhyrirqld eggffiarrt tfrnlqdlve hyskdadglc vnlckpcvqv ekpvteglsh
      241 rtrdqweidr sslkfvrklg qgqfgevweg swnnttavai ktlkpgtmdp kdflaeaqim
      301 kklrhakliq lyavctleep iyiitelmrn gslleylqgk gkslnlqrli dmaaqiaagm
      361 aylesqnyih rdlaarnvlv aelnvvkiad fglarliked eyearvgarf pikwtapeaa
      421 nyskfsiksd vwsfgillte lvtygripyp gmtnaevlhq vehgyrmpcp pgcptalydi
      481 mlecwnkdpm krptfetlqw kledfftmeg seykeasay
//
LOCUS       XP_034190540             519 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190540
VERSION     XP_034190540.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334649.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190540.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="tyrosine-protein kinase Src42A isoform X1"
                     /calculated_mol_wt=59490
     Region          72..123
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(77,79,82..83,86,103..105,118,120,122..123)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          131..226
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(142,162,164..167,172,183,185,218)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(142,162,183,185)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(184,212)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          244..509
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(259,261..263,267,279,281,325..328,332,372,376..377,
                     379,390,407..411,420,454)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(259,262,267,279,281,325..328,332,372,374,377,379,
                     390)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(273,275,307,310,312,326,351,355,383,503)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(361,364..365,428,490..492,494,496..497,500,504,
                     507..508)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(372,376,407..411,420,454)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(389..398,399..413)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..519
                     /gene="Src42A"
                     /coded_by="XM_034334649.2:119..1678"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mhsktfrngi lqrffsryfs sptdtekekp drignpniea vasqaspvpt pppdpirpmp
       61 qipdadvpta kifvalydyd artdedlsfr kgehleilnd tqgdwwlars krtrqegyip
      121 snyvaklksi eaepwyfrki krieaekkll lpendhgafl irdsesrhnd yslsvrdgdt
      181 vkhyrirqld eggffiarrt tfrnlqdlve hyskdadglc vnlckpcvqv ekpvteglsh
      241 rtrdqweidr sslkfvrklg qgqfgevweg swnnttavai ktlkpgtmdp kdflaeaqim
      301 kklrhakliq lyavctleep iyiitelmrn gslleylqgk gkslnlqrli dmaaqiaagm
      361 aylesqnyih rdlaarnvlv aelnvvkiad fglarliked eyearvgarf pikwtapeaa
      421 nyskfsiksd vwsfgillte lvtygripyp gmtnaevlhq vehgyrmpcp pgcptalydi
      481 mlecwnkdpm krptfetlqw kledfftmeg seykeasay
//
LOCUS       XP_076546003             512 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546003
VERSION     XP_076546003.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689888.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="tyrosine-protein kinase Src42A isoform X2"
                     /calculated_mol_wt=58359
     Region          65..116
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(70,72,75..76,79,96..98,111,113,115..116)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          124..219
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(135,155,157..160,165,176,178,211)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(135,155,176,178)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(177,205)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          237..502
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(252,254..256,260,272,274,318..321,325,365,369..370,
                     372,383,400..404,413,447)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(252,255,260,272,274,318..321,325,365,367,370,372,
                     383)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(266,268,300,303,305,319,344,348,376,496)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(354,357..358,421,483..485,487,489..490,493,497,
                     500..501)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(365,369,400..404,413,447)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(382..391,392..406)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..512
                     /gene="Src42A"
                     /coded_by="XM_076689888.1:16..1554"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mgvftspsvs hensptdtek ekpdrignpn ieavasqasp vptpppdpir pmpqipdadv
       61 ptakifvaly dydartdedl sfrkgehlei lndtqgdwwl arskrtrqeg yipsnyvakl
      121 ksieaepwyf rkikrieaek klllpendhg aflirdsesr hndyslsvrd gdtvkhyrir
      181 qldeggffia rrttfrnlqd lvehyskdad glcvnlckpc vqvekpvteg lshrtrdqwe
      241 idrsslkfvr klgqgqfgev wegswnntta vaiktlkpgt mdpkdflaea qimkklrhak
      301 liqlyavctl eepiyiitel mrngslleyl qgkgkslnlq rlidmaaqia agmaylesqn
      361 yihrdlaarn vlvaelnvvk iadfglarli kedeyearvg arfpikwtap eaanyskfsi
      421 ksdvwsfgil ltelvtygri pypgmtnaev lhqvehgyrm pcppgcptal ydimlecwnk
      481 dpmkrptfet lqwkledfft megseykeas ay
//
LOCUS       XP_034190618             505 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190618
VERSION     XP_034190618.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334727.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="tyrosine-protein kinase Src42A isoform X3"
                     /calculated_mol_wt=57625
     Region          58..109
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(63,65,68..69,72,89..91,104,106,108..109)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          117..212
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(128,148,150..153,158,169,171,204)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(128,148,169,171)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(170,198)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          230..495
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(245,247..249,253,265,267,311..314,318,358,362..363,
                     365,376,393..397,406,440)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(245,248,253,265,267,311..314,318,358,360,363,365,
                     376)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(259,261,293,296,298,312,337,341,369,489)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(347,350..351,414,476..478,480,482..483,486,490,
                     493..494)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(358,362,393..397,406,440)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(375..384,385..399)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..505
                     /gene="Src42A"
                     /coded_by="XM_034334727.2:810..2327"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mgncfssptd tekekpdrig npnieavasq aspvptpppd pirpmpqipd advptakifv
       61 alydydartd edlsfrkgeh leilndtqgd wwlarskrtr qegyipsnyv aklksieaep
      121 wyfrkikrie aekklllpen dhgaflirds esrhndysls vrdgdtvkhy rirqldeggf
      181 fiarrttfrn lqdlvehysk dadglcvnlc kpcvqvekpv teglshrtrd qweidrsslk
      241 fvrklgqgqf gevwegswnn ttavaiktlk pgtmdpkdfl aeaqimkklr hakliqlyav
      301 ctleepiyii telmrngsll eylqgkgksl nlqrlidmaa qiaagmayle sqnyihrdla
      361 arnvlvaeln vvkiadfgla rlikedeyea rvgarfpikw tapeaanysk fsiksdvwsf
      421 gilltelvty gripypgmtn aevlhqvehg yrmpcppgcp talydimlec wnkdpmkrpt
      481 fetlqwkled fftmegseyk easay
//
LOCUS       XP_034190694             505 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190694
VERSION     XP_034190694.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334803.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="tyrosine-protein kinase Src42A isoform X3"
                     /calculated_mol_wt=57625
     Region          58..109
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(63,65,68..69,72,89..91,104,106,108..109)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          117..212
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(128,148,150..153,158,169,171,204)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(128,148,169,171)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(170,198)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          230..495
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(245,247..249,253,265,267,311..314,318,358,362..363,
                     365,376,393..397,406,440)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(245,248,253,265,267,311..314,318,358,360,363,365,
                     376)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(259,261,293,296,298,312,337,341,369,489)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(347,350..351,414,476..478,480,482..483,486,490,
                     493..494)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(358,362,393..397,406,440)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(375..384,385..399)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..505
                     /gene="Src42A"
                     /coded_by="XM_034334803.2:829..2346"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mgncfssptd tekekpdrig npnieavasq aspvptpppd pirpmpqipd advptakifv
       61 alydydartd edlsfrkgeh leilndtqgd wwlarskrtr qegyipsnyv aklksieaep
      121 wyfrkikrie aekklllpen dhgaflirds esrhndysls vrdgdtvkhy rirqldeggf
      181 fiarrttfrn lqdlvehysk dadglcvnlc kpcvqvekpv teglshrtrd qweidrsslk
      241 fvrklgqgqf gevwegswnn ttavaiktlk pgtmdpkdfl aeaqimkklr hakliqlyav
      301 ctleepiyii telmrngsll eylqgkgksl nlqrlidmaa qiaagmayle sqnyihrdla
      361 arnvlvaeln vvkiadfgla rlikedeyea rvgarfpikw tapeaanysk fsiksdvwsf
      421 gilltelvty gripypgmtn aevlhqvehg yrmpcppgcp talydimlec wnkdpmkrpt
      481 fetlqwkled fftmegseyk easay
//
LOCUS       XP_034190767             505 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190767
VERSION     XP_034190767.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334876.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="tyrosine-protein kinase Src42A isoform X3"
                     /calculated_mol_wt=57625
     Region          58..109
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(63,65,68..69,72,89..91,104,106,108..109)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          117..212
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(128,148,150..153,158,169,171,204)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(128,148,169,171)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(170,198)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          230..495
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(245,247..249,253,265,267,311..314,318,358,362..363,
                     365,376,393..397,406,440)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(245,248,253,265,267,311..314,318,358,360,363,365,
                     376)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(259,261,293,296,298,312,337,341,369,489)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(347,350..351,414,476..478,480,482..483,486,490,
                     493..494)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(358,362,393..397,406,440)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(375..384,385..399)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..505
                     /gene="Src42A"
                     /coded_by="XM_034334876.2:439..1956"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mgncfssptd tekekpdrig npnieavasq aspvptpppd pirpmpqipd advptakifv
       61 alydydartd edlsfrkgeh leilndtqgd wwlarskrtr qegyipsnyv aklksieaep
      121 wyfrkikrie aekklllpen dhgaflirds esrhndysls vrdgdtvkhy rirqldeggf
      181 fiarrttfrn lqdlvehysk dadglcvnlc kpcvqvekpv teglshrtrd qweidrsslk
      241 fvrklgqgqf gevwegswnn ttavaiktlk pgtmdpkdfl aeaqimkklr hakliqlyav
      301 ctleepiyii telmrngsll eylqgkgksl nlqrlidmaa qiaagmayle sqnyihrdla
      361 arnvlvaeln vvkiadfgla rlikedeyea rvgarfpikw tapeaanysk fsiksdvwsf
      421 gilltelvty gripypgmtn aevlhqvehg yrmpcppgcp talydimlec wnkdpmkrpt
      481 fetlqwkled fftmegseyk easay
//
LOCUS       XP_034190929             505 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190929
VERSION     XP_034190929.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335038.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="tyrosine-protein kinase Src42A isoform X3"
                     /calculated_mol_wt=57625
     Region          58..109
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(63,65,68..69,72,89..91,104,106,108..109)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          117..212
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(128,148,150..153,158,169,171,204)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(128,148,169,171)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(170,198)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          230..495
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(245,247..249,253,265,267,311..314,318,358,362..363,
                     365,376,393..397,406,440)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(245,248,253,265,267,311..314,318,358,360,363,365,
                     376)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(259,261,293,296,298,312,337,341,369,489)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(347,350..351,414,476..478,480,482..483,486,490,
                     493..494)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(358,362,393..397,406,440)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(375..384,385..399)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..505
                     /gene="Src42A"
                     /coded_by="XM_034335038.2:513..2030"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mgncfssptd tekekpdrig npnieavasq aspvptpppd pirpmpqipd advptakifv
       61 alydydartd edlsfrkgeh leilndtqgd wwlarskrtr qegyipsnyv aklksieaep
      121 wyfrkikrie aekklllpen dhgaflirds esrhndysls vrdgdtvkhy rirqldeggf
      181 fiarrttfrn lqdlvehysk dadglcvnlc kpcvqvekpv teglshrtrd qweidrsslk
      241 fvrklgqgqf gevwegswnn ttavaiktlk pgtmdpkdfl aeaqimkklr hakliqlyav
      301 ctleepiyii telmrngsll eylqgkgksl nlqrlidmaa qiaagmayle sqnyihrdla
      361 arnvlvaeln vvkiadfgla rlikedeyea rvgarfpikw tapeaanysk fsiksdvwsf
      421 gilltelvty gripypgmtn aevlhqvehg yrmpcppgcp talydimlec wnkdpmkrpt
      481 fetlqwkled fftmegseyk easay
//
LOCUS       XP_076546004             461 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase Src42A isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546004
VERSION     XP_076546004.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689889.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..461
                     /product="tyrosine-protein kinase Src42A isoform X4"
                     /calculated_mol_wt=52984
     Region          14..65
                     /region_name="SH3_Src_like"
                     /note="Src homology 3 domain of Src kinase-like Protein
                     Tyrosine Kinases; cd11845"
                     /db_xref="CDD:212779"
     Site            order(19,21,24..25,28,45..47,60,62,64..65)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212779"
     Region          73..168
                     /region_name="SH2_Src_Src42"
                     /note="Src homology 2 (SH2) domain found in the Src
                     oncogene at 42A (Src42); cd10370"
                     /db_xref="CDD:198233"
     Site            order(84,104,106..109,114,125,127,160)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198233"
     Site            order(84,104,125,127)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198233"
     Site            order(126,154)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198233"
     Region          186..451
                     /region_name="PTKc_Frk_like"
                     /note="Catalytic domain of Fyn-related kinase-like Protein
                     Tyrosine Kinases; cd05068"
                     /db_xref="CDD:270653"
     Site            order(201,203..205,209,221,223,267..270,274,314,318..319,
                     321,332,349..353,362,396)
                     /site_type="active"
                     /db_xref="CDD:270653"
     Site            order(201,204,209,221,223,267..270,274,314,316,319,321,
                     332)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270653"
     Site            order(215,217,249,252,254,268,293,297,325,445)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(303,306..307,370,432..434,436,438..439,442,446,
                     449..450)
                     /site_type="other"
                     /note="CSK binding interface [polypeptide binding]"
                     /db_xref="CDD:270653"
     Site            order(314,318,349..353,362,396)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270653"
     Site            order(331..340,341..355)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270653"
     CDS             1..461
                     /gene="Src42A"
                     /coded_by="XM_076689889.1:502..1887"
                     /db_xref="GeneID:117608881"
ORIGIN      
        1 mpqipdadvp takifvalyd ydartdedls frkgehleil ndtqgdwwla rskrtrqegy
       61 ipsnyvaklk sieaepwyfr kikrieaekk lllpendhga flirdsesrh ndyslsvrdg
      121 dtvkhyrirq ldeggffiar rttfrnlqdl vehyskdadg lcvnlckpcv qvekpvtegl
      181 shrtrdqwei drsslkfvrk lgqgqfgevw egswnnttav aiktlkpgtm dpkdflaeaq
      241 imkklrhakl iqlyavctle epiyiitelm rngslleylq gkgkslnlqr lidmaaqiaa
      301 gmaylesqny ihrdlaarnv lvaelnvvki adfglarlik edeyearvga rfpikwtape
      361 aanyskfsik sdvwsfgill telvtygrip ypgmtnaevl hqvehgyrmp cppgcptaly
      421 dimlecwnkd pmkrptfetl qwkledfftm egseykeasa y
//
LOCUS       XP_034190241             514 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 14 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190241
VERSION     XP_034190241.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334350.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190241.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="ubiquitin specific protease 14 isoform X1"
                     /calculated_mol_wt=58157
     Region          22..92
                     /region_name="Ubl_USP14_like"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     carboxyl-terminal hydrolase 14 (USP14) and similar
                     proteins; cd16104"
                     /db_xref="CDD:340521"
     Region          123..496
                     /region_name="Peptidase_C19A"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyse bonds involving the carboxyl...; cd02657"
                     /db_xref="CDD:239122"
     Site            order(126,131,450,467)
                     /site_type="active"
                     /db_xref="CDD:239122"
     CDS             1..514
                     /gene="Usp14"
                     /coded_by="XM_034334350.2:239..1783"
                     /db_xref="GeneID:117608748"
ORIGIN      
        1 mvvlfytlsv icidhshqft hvkvkwgkel fpnvevntde epmlfkaqlf altgvqperq
       61 kvmlkgmtlk dddwgniklk dgitvlmmgs keedvpvept ekplfledmn eselataldl
      121 pagltnlgnt cylnatvqcl ktvpelrdal knfsgglaas vssslvpaqs itaalrdlyn
      181 smdkgsslpp vvllqmmhsa fprfaekseh grfqqqdane cwtelirmlq qklpakenpv
      241 tsedngqtyk prslieqyfg gtfdtelkci ecedepptkg keeflqlscf istevkymhs
      301 glrnkmqeql tkmsptlgrd amytktskis rlpayltiqf vrfyykekea inakilkdvk
      361 fplefdafel cgselqsklt pmrekfkvye dslleeshsm kdkkdkvdnd kkkykqeafw
      421 fandigsnns gyytlqavlt hrgrssssgh yvawvrqksd twlkcddetv savtseevlk
      481 lsgggdwhca yvllygpkil evpdtdnned svtk
//
LOCUS       XP_034190320             498 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 14 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190320
VERSION     XP_034190320.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334429.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="ubiquitin specific protease 14 isoform X2"
                     /calculated_mol_wt=56304
     Region          4..76
                     /region_name="Ubl_USP14_like"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     carboxyl-terminal hydrolase 14 (USP14) and similar
                     proteins; cd16104"
                     /db_xref="CDD:340521"
     Region          107..480
                     /region_name="Peptidase_C19A"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyse bonds involving the carboxyl...; cd02657"
                     /db_xref="CDD:239122"
     Site            order(110,115,434,451)
                     /site_type="active"
                     /db_xref="CDD:239122"
     CDS             1..498
                     /gene="Usp14"
                     /coded_by="XM_034334429.2:144..1640"
                     /db_xref="GeneID:117608748"
ORIGIN      
        1 mpqysikvkw gkelfpnvev ntdeepmlfk aqlfaltgvq perqkvmlkg mtlkdddwgn
       61 iklkdgitvl mmgskeedvp veptekplfl edmneselat aldlpagltn lgntcylnat
      121 vqclktvpel rdalknfsgg laasvssslv paqsitaalr dlynsmdkgs slppvvllqm
      181 mhsafprfae ksehgrfqqq danecwteli rmlqqklpak enpvtsedng qtykprslie
      241 qyfggtfdte lkciecedep ptkgkeeflq lscfistevk ymhsglrnkm qeqltkmspt
      301 lgrdamytkt skisrlpayl tiqfvrfyyk ekeainakil kdvkfplefd afelcgselq
      361 skltpmrekf kvyedsllee shsmkdkkdk vdndkkkykq eafwfandig snnsgyytlq
      421 avlthrgrss ssghyvawvr qksdtwlkcd detvsavtse evlklsgggd whcayvllyg
      481 pkilevpdtd nnedsvtk
//
LOCUS       XP_034190406             433 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 14 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190406
VERSION     XP_034190406.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334515.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190406.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="ubiquitin specific protease 14 isoform X3"
                     /calculated_mol_wt=49497
     Region          22..92
                     /region_name="Ubl_USP14_like"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     carboxyl-terminal hydrolase 14 (USP14) and similar
                     proteins; cd16104"
                     /db_xref="CDD:340521"
     Region          123..380
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     CDS             1..433
                     /gene="Usp14"
                     /coded_by="XM_034334515.2:509..1810"
                     /db_xref="GeneID:117608748"
ORIGIN      
        1 mvvlfytlsv icidhshqft hvkvkwgkel fpnvevntde epmlfkaqlf altgvqperq
       61 kvmlkgmtlk dddwgniklk dgitvlmmgs keedvpvept ekplfledmn eselataldl
      121 pagltnlgnt cylnatvqcl ktvpelrdal knfsgglaas vssslvpaqs itaalrdlyn
      181 smdkgsslpp vvllqmmhsa fprfaekseh grfqqqdane cwtelirmlq qklpakenpv
      241 tsedngqtyk prslieqyfg gtfdtelkci ecedepptkg keeflqlscf istevkymhs
      301 glrnkmqeql tkmsptlgrd amytktskis rlpayltiqf vrfyykekea inakilkdvk
      361 fplefdafel cgselqsklt pmrekfkvye dslleeshsm kdkkdkvdnd kkkykqeafw
      421 fandycnfry rvk
//
LOCUS       XP_034173695             233 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601255 [Osmia lignaria lignaria].
ACCESSION   XP_034173695
VERSION     XP_034173695.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317804.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="uncharacterized protein LOC117601255"
                     /calculated_mol_wt=27032
     Region          44..186
                     /region_name="Ins_allergen_rp"
                     /note="Insect allergen related repeat, nitrile-specifier
                     detoxification; pfam06757"
                     /db_xref="CDD:462003"
     CDS             1..233
                     /gene="LOC117601255"
                     /coded_by="XM_034317804.2:470..1171"
                     /db_xref="GeneID:117601255"
ORIGIN      
        1 mnphimiasv matasvalig flaykigfrs ndsfnlplln ddssleedlr dilafvpfne
       61 vkeivekymk ydaqigdtvs fvsdqkrfiv refqrmpqln klivslrqdg lnvdswyekv
      121 rsfwktsprf vrndpsiakg gltvminttl ntipldelhe fllqkvkysr sfrrflqmlk
      181 skdfrdfcka vednavlhhh yfwakesgle itfaielfte lhtyftqtll lqg
//
LOCUS       XP_034173714             233 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601255 [Osmia lignaria lignaria].
ACCESSION   XP_034173714
VERSION     XP_034173714.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317823.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="uncharacterized protein LOC117601255"
                     /calculated_mol_wt=27032
     Region          44..186
                     /region_name="Ins_allergen_rp"
                     /note="Insect allergen related repeat, nitrile-specifier
                     detoxification; pfam06757"
                     /db_xref="CDD:462003"
     CDS             1..233
                     /gene="LOC117601255"
                     /coded_by="XM_034317823.2:466..1167"
                     /db_xref="GeneID:117601255"
ORIGIN      
        1 mnphimiasv matasvalig flaykigfrs ndsfnlplln ddssleedlr dilafvpfne
       61 vkeivekymk ydaqigdtvs fvsdqkrfiv refqrmpqln klivslrqdg lnvdswyekv
      121 rsfwktsprf vrndpsiakg gltvminttl ntipldelhe fllqkvkysr sfrrflqmlk
      181 skdfrdfcka vednavlhhh yfwakesgle itfaielfte lhtyftqtll lqg
//
LOCUS       XP_034173704             233 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601255 [Osmia lignaria lignaria].
ACCESSION   XP_034173704
VERSION     XP_034173704.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317813.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="uncharacterized protein LOC117601255"
                     /calculated_mol_wt=27032
     Region          44..186
                     /region_name="Ins_allergen_rp"
                     /note="Insect allergen related repeat, nitrile-specifier
                     detoxification; pfam06757"
                     /db_xref="CDD:462003"
     CDS             1..233
                     /gene="LOC117601255"
                     /coded_by="XM_034317813.2:450..1151"
                     /db_xref="GeneID:117601255"
ORIGIN      
        1 mnphimiasv matasvalig flaykigfrs ndsfnlplln ddssleedlr dilafvpfne
       61 vkeivekymk ydaqigdtvs fvsdqkrfiv refqrmpqln klivslrqdg lnvdswyekv
      121 rsfwktsprf vrndpsiakg gltvminttl ntipldelhe fllqkvkysr sfrrflqmlk
      181 skdfrdfcka vednavlhhh yfwakesgle itfaielfte lhtyftqtll lqg
//
LOCUS       XP_034173810             216 aa            linear   INV 03-SEP-2025
DEFINITION  protein G12-like [Osmia lignaria lignaria].
ACCESSION   XP_034173810
VERSION     XP_034173810.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..216
                     /product="protein G12-like"
                     /calculated_mol_wt=24186
     Region          29..197
                     /region_name="Ins_allergen_rp"
                     /note="Insect allergen related repeat, nitrile-specifier
                     detoxification; pfam06757"
                     /db_xref="CDD:462003"
     CDS             1..216
                     /gene="LOC117601305"
                     /coded_by="XM_034317919.2:162..812"
                     /db_xref="GeneID:117601305"
ORIGIN      
        1 mkftlailav lavvgqlnay nipstgsgel akdfqdilnl ipldelidit rtyaaqdkdf
       61 qlmtnllksp dskefitfve gapefkklma yaqnnglniy sllnkyneil nippfkplag
      121 pqraitggls gyvqdilelv pvdemldmqk dkmenskafk glineitsae flnfynsvlq
      181 seilsklenr aervgvdkes fhhyypifli lapfnk
//
LOCUS       XP_034178383             863 aa            linear   INV 03-SEP-2025
DEFINITION  chromosome transmission fidelity protein 18 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034178383
VERSION     XP_034178383.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322492.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178383.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..863
                     /product="chromosome transmission fidelity protein 18
                     homolog"
                     /calculated_mol_wt=98579
     Region          253..>531
                     /region_name="PRK04195"
                     /note="replication factor C large subunit; Provisional"
                     /db_xref="CDD:235250"
     Region          281..>348
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     CDS             1..863
                     /gene="cutlet"
                     /coded_by="XM_034322492.2:404..2995"
                     /db_xref="GeneID:117603383"
ORIGIN      
        1 mdsefpdpde efdlvhedey ellkeieele ekklaeskrn ktvqekslqk nesntsitnv
       61 skelntpipn ntqrnlctps tskiiecnsn aeidntkkrg ydeifgdisd iletnefggf
      121 eepkekkprw depqdiikav ldarekfhef srgvsgkknh ikdtresisl kvprwnfiav
      181 trphdaqriy vrvksdekni ikrnttsisn llsipysqlk aeaeeiiien vkrascense
      241 vsvietnend elwvdkyrpr syiellsdes vnrhllhwik lwdkvvfnrn yvpskkeklq
      301 stfgnrkfik nevfeevdss gfpirrvvll tgppglgktt lahlaakhag ynvveinasd
      361 ernpdafrqv llastqmkav mgndprpncl vldeidgapa asidllikfv qgklttkskk
      421 dktksnkksn ichrpvicic nepytpslra lraialiipv pevslarlad rlmkisqreq
      481 lkvnphalmk lveisgcdir sclgalqymg girlgdnlsf alkdsrkglf dswkqiltip
      541 vnkngilpis ervrlvlktv qqgeteklaq gifhnypevc neklnyistc lqwfqfydei
      601 cssvghlqnw simpytnyaf vtwhlyfska rtvrlsypsi vyeaqqksek sigiltsvhr
      661 nsgcdstvla vdiapflpdl lsprlrtisg hlhsnkekdd iarivnvllq fglsftqers
      721 ldgiynykld pnifeiaifp gckqhrslay svkqiivqel daerlrrsan aikvitnstq
      781 nenkniseqn vttkpvgsev aideqtktke ivykdffgrv ikvnpdkqkt tnailmekyp
      841 vwykykqgys navrrfikmk dfl
//
LOCUS       XP_034178397             642 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603390 [Osmia lignaria lignaria].
ACCESSION   XP_034178397
VERSION     XP_034178397.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322506.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178397.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..642
                     /product="uncharacterized protein LOC117603390"
                     /calculated_mol_wt=74083
     Region          462..606
                     /region_name="WS_DGAT_C"
                     /note="WS/DGAT C-terminal domain; pfam06974"
                     /db_xref="CDD:462057"
     CDS             1..642
                     /gene="LOC117603390"
                     /coded_by="XM_034322506.2:252..2180"
                     /db_xref="GeneID:117603390"
ORIGIN      
        1 mskiyqvllt fytilfspli vlllacsyly rrivnvilri qlkekfagfl ngtdcvwaie
       61 epsalsvnni lliiekdarh snikflesfr qmaktrilss pleklfcrrk qkygyyywer
      121 cdeidlkkrv rwleyertsc dgscdniysg hlkrvlwnvc nqplpenhta sweiligkcc
      181 prssyhylrr meeritskik ipvlfrvhhs lgdgmsllkl freaiidkep iqetylqlen
      241 asfmpmsdet kkpnittipl kaylngdksl qcsfqkdivs asmpfihfgv awqfvkssrg
      301 rmdvavkrfq nitvedlkre tkafvkkhvd nlrvlflkiv ckkvkkclrm triiidapac
      361 liqqafrsmd ksalhgaqls geklvsywle ddfkntidqk lftkiqniki itgarfgdvi
      421 laalsvslhk yflrmnepip skisvvlpmk ieewnenlpl qnnfsvgvlp lcvsqingeq
      481 lpdpaensqi lerledikka ndelrnsldy tvnflvmkyl sallpdkflr pilkshstmv
      541 fsnlvgprev kllghslkni vfwipnrsft gigfslltyr gylhlsliad kaliqnekal
      601 trilentvse idnlydkltl sffskklhrs mstpmkkgvg vl
//
LOCUS       XP_034178451             443 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603401 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178451
VERSION     XP_034178451.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322560.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178451.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..443
                     /product="uncharacterized protein LOC117603401 isoform X1"
                     /calculated_mol_wt=50399
     CDS             1..443
                     /gene="LOC117603401"
                     /coded_by="XM_034322560.2:305..1636"
                     /db_xref="GeneID:117603401"
ORIGIN      
        1 msarsqrgvh ettikrfptp rvnqpnrapk paitydydaa vsqpdvpdfl nfkkagrgvm
       61 vsgvplkkra lktrlrcrim srknfqkrsq tfrprsvvqr hakvpdrryt kkpiassdvs
      121 eeetdvleke nvtdngtnid dssvyrkkda knvykdgnet iesigdnqit grkekherpd
      181 dcdsvtaksp vlqgksskip rakttafpvq elhsqtkktg nkvknstcln ctsincapnv
      241 evlereveri ankiqgrsce qdvvrfeefq nvakmwnkee ssarkgeeet eddrgltstd
      301 dspaiskind rrvatilrsl klncpckscs nheplsssrq eddsdlksts pdksrkkrwp
      361 lkhrkyldts tedvdassdd neendkkkkr nrlkakvplm qqvktssiin tspkistrrt
      421 sfsflntlfd ivfwpylflk tnr
//
LOCUS       XP_034178424             443 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603401 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178424
VERSION     XP_034178424.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322533.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178424.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..443
                     /product="uncharacterized protein LOC117603401 isoform X1"
                     /calculated_mol_wt=50399
     CDS             1..443
                     /gene="LOC117603401"
                     /coded_by="XM_034322533.2:284..1615"
                     /db_xref="GeneID:117603401"
ORIGIN      
        1 msarsqrgvh ettikrfptp rvnqpnrapk paitydydaa vsqpdvpdfl nfkkagrgvm
       61 vsgvplkkra lktrlrcrim srknfqkrsq tfrprsvvqr hakvpdrryt kkpiassdvs
      121 eeetdvleke nvtdngtnid dssvyrkkda knvykdgnet iesigdnqit grkekherpd
      181 dcdsvtaksp vlqgksskip rakttafpvq elhsqtkktg nkvknstcln ctsincapnv
      241 evlereveri ankiqgrsce qdvvrfeefq nvakmwnkee ssarkgeeet eddrgltstd
      301 dspaiskind rrvatilrsl klncpckscs nheplsssrq eddsdlksts pdksrkkrwp
      361 lkhrkyldts tedvdassdd neendkkkkr nrlkakvplm qqvktssiin tspkistrrt
      421 sfsflntlfd ivfwpylflk tnr
//
LOCUS       XP_034178432             442 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603401 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178432
VERSION     XP_034178432.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322541.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178432.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..442
                     /product="uncharacterized protein LOC117603401 isoform X2"
                     /calculated_mol_wt=50271
     CDS             1..442
                     /gene="LOC117603401"
                     /coded_by="XM_034322541.2:305..1633"
                     /db_xref="GeneID:117603401"
ORIGIN      
        1 msarsqrgvh ettikrfptp rvnqpnrapk paitydydaa vsqpdvpdfl nfkkagrgvm
       61 vsgvplkkra lktrlrcrim srknfqkrsq tfrprsvvqr hakvpdrryt kkpiassdvs
      121 eeetdvleke nvtdngtnid dssvyrkkda knvykdgnet iesigdnqit grkekherpd
      181 dcdsvtaksp vlqgksskip rakttafpvq elhstkktgn kvknstclnc tsincapnve
      241 vlereveria nkiqgrsceq dvvrfeefqn vakmwnkees sarkgeeete ddrgltstdd
      301 spaiskindr rvatilrslk lncpckscsn heplsssrqe ddsdlkstsp dksrkkrwpl
      361 khrkyldtst edvdassddn eendkkkkrn rlkakvplmq qvktssiint spkistrrts
      421 fsflntlfdi vfwpylflkt nr
//
LOCUS       XP_034178442             417 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603401 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178442
VERSION     XP_034178442.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322551.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178442.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..417
                     /product="uncharacterized protein LOC117603401 isoform X3"
                     /calculated_mol_wt=47330
     CDS             1..417
                     /gene="LOC117603401"
                     /coded_by="XM_034322551.2:305..1558"
                     /db_xref="GeneID:117603401"
ORIGIN      
        1 msarsqrgvh ettikrfptp rvnqpnrapk paitydydaa vsqpdvpdfl nfkkagrgvm
       61 krsqtfrprs vvqrhakvpd rrytkkpias sdvseeetdv lekenvtdng tniddssvyr
      121 kkdaknvykd gnetiesigd nqitgrkekh erpddcdsvt akspvlqgks skipraktta
      181 fpvqelhsqt kktgnkvkns tclnctsinc apnvevlere veriankiqg rsceqdvvrf
      241 eefqnvakmw nkeessarkg eeeteddrgl tstddspais kindrrvati lrslklncpc
      301 kscsnhepls ssrqeddsdl kstspdksrk krwplkhrky ldtstedvda ssddneendk
      361 kkkrnrlkak vplmqqvkts siintspkis trrtsfsfln tlfdivfwpy lflktnr
//
LOCUS       XP_034178465             307 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal Membrane Protein 34 [Osmia lignaria lignaria].
ACCESSION   XP_034178465
VERSION     XP_034178465.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="peroxisomal Membrane Protein 34"
                     /calculated_mol_wt=34295
     Region          11..96
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          105..195
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          204..300
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..307
                     /gene="PMP34"
                     /coded_by="XM_034322574.2:447..1370"
                     /db_xref="GeneID:117603415"
ORIGIN      
        1 mgggaqntgi ftyetlvhai sgaagsvvam atffpldtvr srlqleedre skstlatire
       61 lvdkegvctl yrgmvpvlqs lcasnfvyfy tfhglkmlra rrnqtagndl lfasiagiin
      121 vlsttplwvv ntrlkmrgvg qvqernnney ntlydglmhi wkyeglsklw sgtlpslmlv
      181 tnpaiqfmty etlkrkvtas lrnsqppawi ffaigavaka istaltypiq lvqtklrhgy
      241 kypnlppdag tleiifyilk kqgigalykg meakllqtil taalmflaye kisrfvfril
      301 lhrpilr
//
LOCUS       XP_076546005             307 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal Membrane Protein 34 [Osmia lignaria lignaria].
ACCESSION   XP_076546005
VERSION     XP_076546005.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689890.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="peroxisomal Membrane Protein 34"
                     /calculated_mol_wt=34295
     Region          11..96
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          105..195
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          204..300
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..307
                     /gene="PMP34"
                     /coded_by="XM_076689890.1:611..1534"
                     /db_xref="GeneID:117603415"
ORIGIN      
        1 mgggaqntgi ftyetlvhai sgaagsvvam atffpldtvr srlqleedre skstlatire
       61 lvdkegvctl yrgmvpvlqs lcasnfvyfy tfhglkmlra rrnqtagndl lfasiagiin
      121 vlsttplwvv ntrlkmrgvg qvqernnney ntlydglmhi wkyeglsklw sgtlpslmlv
      181 tnpaiqfmty etlkrkvtas lrnsqppawi ffaigavaka istaltypiq lvqtklrhgy
      241 kypnlppdag tleiifyilk kqgigalykg meakllqtil taalmflaye kisrfvfril
      301 lhrpilr
//
LOCUS       XP_076546006             307 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal Membrane Protein 34 [Osmia lignaria lignaria].
ACCESSION   XP_076546006
VERSION     XP_076546006.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689891.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="peroxisomal Membrane Protein 34"
                     /calculated_mol_wt=34295
     Region          11..96
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          105..195
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          204..300
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..307
                     /gene="PMP34"
                     /coded_by="XM_076689891.1:608..1531"
                     /db_xref="GeneID:117603415"
ORIGIN      
        1 mgggaqntgi ftyetlvhai sgaagsvvam atffpldtvr srlqleedre skstlatire
       61 lvdkegvctl yrgmvpvlqs lcasnfvyfy tfhglkmlra rrnqtagndl lfasiagiin
      121 vlsttplwvv ntrlkmrgvg qvqernnney ntlydglmhi wkyeglsklw sgtlpslmlv
      181 tnpaiqfmty etlkrkvtas lrnsqppawi ffaigavaka istaltypiq lvqtklrhgy
      241 kypnlppdag tleiifyilk kqgigalykg meakllqtil taalmflaye kisrfvfril
      301 lhrpilr
//
LOCUS       XP_076546007             307 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal Membrane Protein 34 [Osmia lignaria lignaria].
ACCESSION   XP_076546007
VERSION     XP_076546007.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689892.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="peroxisomal Membrane Protein 34"
                     /calculated_mol_wt=34295
     Region          11..96
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          105..195
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          204..300
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..307
                     /gene="PMP34"
                     /coded_by="XM_076689892.1:606..1529"
                     /db_xref="GeneID:117603415"
ORIGIN      
        1 mgggaqntgi ftyetlvhai sgaagsvvam atffpldtvr srlqleedre skstlatire
       61 lvdkegvctl yrgmvpvlqs lcasnfvyfy tfhglkmlra rrnqtagndl lfasiagiin
      121 vlsttplwvv ntrlkmrgvg qvqernnney ntlydglmhi wkyeglsklw sgtlpslmlv
      181 tnpaiqfmty etlkrkvtas lrnsqppawi ffaigavaka istaltypiq lvqtklrhgy
      241 kypnlppdag tleiifyilk kqgigalykg meakllqtil taalmflaye kisrfvfril
      301 lhrpilr
//
LOCUS       XP_034178501             163 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603431 [Osmia lignaria lignaria].
ACCESSION   XP_034178501
VERSION     XP_034178501.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322610.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..163
                     /product="uncharacterized protein LOC117603431"
                     /calculated_mol_wt=18390
     CDS             1..163
                     /gene="LOC117603431"
                     /coded_by="XM_034322610.2:226..717"
                     /db_xref="GeneID:117603431"
ORIGIN      
        1 mqtslskqai gcigkctsgl npeeldqitd ninktlahpq greifkkylv qrdlrdnlec
       61 lelyetccdf idqekscshs kkesslesli envtrvkema edldgvpqid mallerfnea
      121 lnsdsrtall gvlvdtrdrs rdhlrrahvg fkkyasepcp mtk
//
LOCUS       XP_034178524             260 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603455 [Osmia lignaria lignaria].
ACCESSION   XP_034178524
VERSION     XP_034178524.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322633.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178524.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="uncharacterized protein LOC117603455"
                     /calculated_mol_wt=29923
     CDS             1..260
                     /gene="LOC117603455"
                     /coded_by="XM_034322633.2:1..783"
                     /db_xref="GeneID:117603455"
ORIGIN      
        1 myqtsvensc trkisiatee yvmkindird tvssleeslm vsrsleaerw llysnlktkn
       61 kvamddlkka ndhnknmlnl frqsvtdirl gsqtalpesl krevqrnwhq kydtllkqne
      121 qlkeevitkn avikekalei dnlqqklltl gdrlvernea venickkyln lkkrkdeqei
      181 llrgsietlq dalknknsvt gnksaemslm pskdallare rrrsdrlaye nsllrvllqe
      241 akrgyqvsgs ssvisfektt
//
LOCUS       XP_034192046            1300 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 8 [Osmia lignaria lignaria].
ACCESSION   XP_034192046
VERSION     XP_034192046.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336155.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192046.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1300
                     /product="vacuolar protein sorting 8"
                     /calculated_mol_wt=144416
     Region          <165..235
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          169..205
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          578..761
                     /region_name="Vps8"
                     /note="Golgi CORVET complex core vacuolar protein 8;
                     pfam12816"
                     /db_xref="CDD:463718"
     Region          952..1067
                     /region_name="CLH"
                     /note="Clathrin heavy chain repeat homology; smart00299"
                     /db_xref="CDD:128594"
     Region          1209..1246
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(1209,1212,1228,1230,1233,1236,1243,1246)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438348"
     CDS             1..1300
                     /gene="Vps8"
                     /coded_by="XM_034336155.2:93..3995"
                     /db_xref="GeneID:117609581"
ORIGIN      
        1 maenglgsda gsqeylatei inldieeldn teyaipavee lpslesilte pdcaslsgtd
       61 ndfgltpgek lgssettsvg shlslnslnk snktsqstss gtilrhvilk gissqivsas
      121 ekvnaglasa vaaggnmlvv gtshglilgf dssqtlrwcd qearhqgsvs alcfnydgsr
      181 vlagfarghi lmidssngkv lrtltdvhpl dtavlhvkft dspkvalcsd sggsvfelif
      241 trvmgvrgcd srclfsgsrg evctleplll nhlpshplkn ytlvamatls kvivvcirpr
      301 mrvvlthpls gapiappqls wqlvviqaad asrvidpvla larddvvhfy qvcteagsri
      361 klfplrrmtf pytisnlrwl nprslavldt qerlhlvdvr aqdnletldm srvrisyass
      421 hfkglstggn vskamalage racyntvvtf gtqllllgtk slhvvcirtw terlrhltiq
      481 rrfpealalg lsfyqdkgka vvglrgskqr rkqiardkvc qvliqymeel nhccsdetse
      541 feivttcvdy ciqlentdll fgklwdlvse seglkasylh alesplldgs lrprlpplia
      601 qqlvtlydqe ekidslqaii vlldvdcldi hqvttvcrqr clwealihlq ttalgdftap
      661 ihqlvpilhn lltnlkdglt rdsiqlgnal lvyascclag rgfprdelpe gmsqraktdv
      721 lrallsqhss landterqyp ylrtllqfda kgfldviaia fqepeftsem glrqrqrlid
      781 illnivmpst plsprnpdyi ndehrnlvli fianevaent vnlepsmlnk mieilctdss
      841 mgsskelnad kedailgllr skkirnisdn tllnlaeran ftrvaellys aredwisvck
      901 cmishssrhr dvwvwlknlp anslqsvima nvealvgina sqfailvath mpnrvdeile
      961 klesipnlqy allealyqiv qykeeditfe lktelfekyl elmcelrpdr viehiqgfhg
     1021 crlnealkiv qktnhkdaea vmleklgnyq dafdilikef qnhlelycqn kslentsiht
     1081 aiqlaglcrr sagnldwmpl veiilqthsn skdekvekls gkllritlef lsgttalstv
     1141 leqilkhpla tsgtigdirq llsgvlthsr yeqtlvetta rlvslelhka lkkslrdagr
     1201 acasasiica icrhllsqct dyiivfscgh gfhsecleep kccykclntk gwaslvtntv
     1261 yttksfhetk qilppefmrn kdlslrlapp tslpdlegif
//
LOCUS       XP_076546009             881 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific peptidase 36 [Osmia lignaria lignaria].
ACCESSION   XP_076546009
VERSION     XP_076546009.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689894.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="ubiquitin specific peptidase 36"
                     /calculated_mol_wt=98280
     Region          123..429
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(128,133,391,408)
                     /site_type="active"
                     /db_xref="CDD:239072"
     CDS             1..881
                     /gene="scny"
                     /coded_by="XM_076689894.1:502..3147"
                     /db_xref="GeneID:117609667"
ORIGIN      
        1 mpavsicdpv aatlcrslet nqpkssddls sclvnnasri lqseiqykev ddyqttildq
       61 lkakyivlsl pnsenldgkt kdgtncikrn ektskgpslp epsvvlyppk kvslgwketf
      121 pvgagmynvg ntcylnstlq alfhipalvn wllsdshhts kceqndggge cltcavaktl
      181 qfshqksgca ikpfyiynkl klicrtmvpg qqedaheflr yllegmekay larhkaskld
      241 syskettpin qifggyirte vkclqcrhvs ttfqhfqdll vdirkantld ealtsyfsre
      301 qldnndykce ackkrvpatk qfslerppkv lcvqlkrfsv lggkiskhig fkqtidmgpy
      361 lwrepgeavq pltyklmsiv thmgpsvncg hytavakvst gqyysfddsc vrpislnnvl
      421 stnayimife meqqtptpvq tqqtvklngt mtvnntlssp gspknktvap kpstsglisl
      481 ngslngtsvn kdnteksets lqastnksav lqftsqkstn figpqlpqkl qdksqprlim
      541 hikngkilng nslvpydgss eeedvnsivp lknhtsssta pktntstsat ngtpknspik
      601 nnlpktvfss kevqkftska nggnnvsqnq ssitqcakaq ngssysttpi myqngklean
      661 gktessstnk skwhhsvrak tgqdekvitk aansnmkgwq vskdtfspts taapngwsvt
      721 dkedvnkvnq nsttpsvaal rdfngtnrsd tvshllkmsh rgygsssvvn wngnrthldr
      781 evdndrreer krhfntddee idrgrvkkvk dhrtyedrsn tdynpfqeyq nsktwnrasg
      841 ggyyrkhyyn tssytrsrhg rnyrpqhyry hnhshghwqr g
//
LOCUS       XP_034192188             881 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific peptidase 36 [Osmia lignaria lignaria].
ACCESSION   XP_034192188
VERSION     XP_034192188.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336297.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="ubiquitin specific peptidase 36"
                     /calculated_mol_wt=98280
     Region          123..429
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(128,133,391,408)
                     /site_type="active"
                     /db_xref="CDD:239072"
     CDS             1..881
                     /gene="scny"
                     /coded_by="XM_034336297.2:399..3044"
                     /db_xref="GeneID:117609667"
ORIGIN      
        1 mpavsicdpv aatlcrslet nqpkssddls sclvnnasri lqseiqykev ddyqttildq
       61 lkakyivlsl pnsenldgkt kdgtncikrn ektskgpslp epsvvlyppk kvslgwketf
      121 pvgagmynvg ntcylnstlq alfhipalvn wllsdshhts kceqndggge cltcavaktl
      181 qfshqksgca ikpfyiynkl klicrtmvpg qqedaheflr yllegmekay larhkaskld
      241 syskettpin qifggyirte vkclqcrhvs ttfqhfqdll vdirkantld ealtsyfsre
      301 qldnndykce ackkrvpatk qfslerppkv lcvqlkrfsv lggkiskhig fkqtidmgpy
      361 lwrepgeavq pltyklmsiv thmgpsvncg hytavakvst gqyysfddsc vrpislnnvl
      421 stnayimife meqqtptpvq tqqtvklngt mtvnntlssp gspknktvap kpstsglisl
      481 ngslngtsvn kdnteksets lqastnksav lqftsqkstn figpqlpqkl qdksqprlim
      541 hikngkilng nslvpydgss eeedvnsivp lknhtsssta pktntstsat ngtpknspik
      601 nnlpktvfss kevqkftska nggnnvsqnq ssitqcakaq ngssysttpi myqngklean
      661 gktessstnk skwhhsvrak tgqdekvitk aansnmkgwq vskdtfspts taapngwsvt
      721 dkedvnkvnq nsttpsvaal rdfngtnrsd tvshllkmsh rgygsssvvn wngnrthldr
      781 evdndrreer krhfntddee idrgrvkkvk dhrtyedrsn tdynpfqeyq nsktwnrasg
      841 ggyyrkhyyn tssytrsrhg rnyrpqhyry hnhshghwqr g
//
LOCUS       XP_076546010             881 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific peptidase 36 [Osmia lignaria lignaria].
ACCESSION   XP_076546010
VERSION     XP_076546010.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689895.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="ubiquitin specific peptidase 36"
                     /calculated_mol_wt=98280
     Region          123..429
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(128,133,391,408)
                     /site_type="active"
                     /db_xref="CDD:239072"
     CDS             1..881
                     /gene="scny"
                     /coded_by="XM_076689895.1:436..3081"
                     /db_xref="GeneID:117609667"
ORIGIN      
        1 mpavsicdpv aatlcrslet nqpkssddls sclvnnasri lqseiqykev ddyqttildq
       61 lkakyivlsl pnsenldgkt kdgtncikrn ektskgpslp epsvvlyppk kvslgwketf
      121 pvgagmynvg ntcylnstlq alfhipalvn wllsdshhts kceqndggge cltcavaktl
      181 qfshqksgca ikpfyiynkl klicrtmvpg qqedaheflr yllegmekay larhkaskld
      241 syskettpin qifggyirte vkclqcrhvs ttfqhfqdll vdirkantld ealtsyfsre
      301 qldnndykce ackkrvpatk qfslerppkv lcvqlkrfsv lggkiskhig fkqtidmgpy
      361 lwrepgeavq pltyklmsiv thmgpsvncg hytavakvst gqyysfddsc vrpislnnvl
      421 stnayimife meqqtptpvq tqqtvklngt mtvnntlssp gspknktvap kpstsglisl
      481 ngslngtsvn kdnteksets lqastnksav lqftsqkstn figpqlpqkl qdksqprlim
      541 hikngkilng nslvpydgss eeedvnsivp lknhtsssta pktntstsat ngtpknspik
      601 nnlpktvfss kevqkftska nggnnvsqnq ssitqcakaq ngssysttpi myqngklean
      661 gktessstnk skwhhsvrak tgqdekvitk aansnmkgwq vskdtfspts taapngwsvt
      721 dkedvnkvnq nsttpsvaal rdfngtnrsd tvshllkmsh rgygsssvvn wngnrthldr
      781 evdndrreer krhfntddee idrgrvkkvk dhrtyedrsn tdynpfqeyq nsktwnrasg
      841 ggyyrkhyyn tssytrsrhg rnyrpqhyry hnhshghwqr g
//
LOCUS       XP_034192250             881 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific peptidase 36 [Osmia lignaria lignaria].
ACCESSION   XP_034192250
VERSION     XP_034192250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336359.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="ubiquitin specific peptidase 36"
                     /calculated_mol_wt=98280
     Region          123..429
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(128,133,391,408)
                     /site_type="active"
                     /db_xref="CDD:239072"
     CDS             1..881
                     /gene="scny"
                     /coded_by="XM_034336359.2:520..3165"
                     /db_xref="GeneID:117609667"
ORIGIN      
        1 mpavsicdpv aatlcrslet nqpkssddls sclvnnasri lqseiqykev ddyqttildq
       61 lkakyivlsl pnsenldgkt kdgtncikrn ektskgpslp epsvvlyppk kvslgwketf
      121 pvgagmynvg ntcylnstlq alfhipalvn wllsdshhts kceqndggge cltcavaktl
      181 qfshqksgca ikpfyiynkl klicrtmvpg qqedaheflr yllegmekay larhkaskld
      241 syskettpin qifggyirte vkclqcrhvs ttfqhfqdll vdirkantld ealtsyfsre
      301 qldnndykce ackkrvpatk qfslerppkv lcvqlkrfsv lggkiskhig fkqtidmgpy
      361 lwrepgeavq pltyklmsiv thmgpsvncg hytavakvst gqyysfddsc vrpislnnvl
      421 stnayimife meqqtptpvq tqqtvklngt mtvnntlssp gspknktvap kpstsglisl
      481 ngslngtsvn kdnteksets lqastnksav lqftsqkstn figpqlpqkl qdksqprlim
      541 hikngkilng nslvpydgss eeedvnsivp lknhtsssta pktntstsat ngtpknspik
      601 nnlpktvfss kevqkftska nggnnvsqnq ssitqcakaq ngssysttpi myqngklean
      661 gktessstnk skwhhsvrak tgqdekvitk aansnmkgwq vskdtfspts taapngwsvt
      721 dkedvnkvnq nsttpsvaal rdfngtnrsd tvshllkmsh rgygsssvvn wngnrthldr
      781 evdndrreer krhfntddee idrgrvkkvk dhrtyedrsn tdynpfqeyq nsktwnrasg
      841 ggyyrkhyyn tssytrsrhg rnyrpqhyry hnhshghwqr g
//
LOCUS       XP_034192319             881 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific peptidase 36 [Osmia lignaria lignaria].
ACCESSION   XP_034192319
VERSION     XP_034192319.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336428.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="ubiquitin specific peptidase 36"
                     /calculated_mol_wt=98280
     Region          123..429
                     /region_name="Peptidase_C19"
                     /note="Peptidase C19 contains ubiquitinyl hydrolases. They
                     are intracellular peptidases that remove ubiquitin
                     molecules from polyubiquinated peptides by cleavage of
                     isopeptide bonds. They hydrolyse bonds involving the
                     carboxyl group of the C-terminal Gly...; cl02553"
                     /db_xref="CDD:470612"
     Site            order(128,133,391,408)
                     /site_type="active"
                     /db_xref="CDD:239072"
     CDS             1..881
                     /gene="scny"
                     /coded_by="XM_034336428.2:220..2865"
                     /db_xref="GeneID:117609667"
ORIGIN      
        1 mpavsicdpv aatlcrslet nqpkssddls sclvnnasri lqseiqykev ddyqttildq
       61 lkakyivlsl pnsenldgkt kdgtncikrn ektskgpslp epsvvlyppk kvslgwketf
      121 pvgagmynvg ntcylnstlq alfhipalvn wllsdshhts kceqndggge cltcavaktl
      181 qfshqksgca ikpfyiynkl klicrtmvpg qqedaheflr yllegmekay larhkaskld
      241 syskettpin qifggyirte vkclqcrhvs ttfqhfqdll vdirkantld ealtsyfsre
      301 qldnndykce ackkrvpatk qfslerppkv lcvqlkrfsv lggkiskhig fkqtidmgpy
      361 lwrepgeavq pltyklmsiv thmgpsvncg hytavakvst gqyysfddsc vrpislnnvl
      421 stnayimife meqqtptpvq tqqtvklngt mtvnntlssp gspknktvap kpstsglisl
      481 ngslngtsvn kdnteksets lqastnksav lqftsqkstn figpqlpqkl qdksqprlim
      541 hikngkilng nslvpydgss eeedvnsivp lknhtsssta pktntstsat ngtpknspik
      601 nnlpktvfss kevqkftska nggnnvsqnq ssitqcakaq ngssysttpi myqngklean
      661 gktessstnk skwhhsvrak tgqdekvitk aansnmkgwq vskdtfspts taapngwsvt
      721 dkedvnkvnq nsttpsvaal rdfngtnrsd tvshllkmsh rgygsssvvn wngnrthldr
      781 evdndrreer krhfntddee idrgrvkkvk dhrtyedrsn tdynpfqeyq nsktwnrasg
      841 ggyyrkhyyn tssytrsrhg rnyrpqhyry hnhshghwqr g
//
LOCUS       XP_034192490             258 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609837 [Osmia lignaria lignaria].
ACCESSION   XP_034192490
VERSION     XP_034192490.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336599.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..258
                     /product="uncharacterized protein LOC117609837"
                     /calculated_mol_wt=29805
     CDS             1..258
                     /gene="LOC117609837"
                     /coded_by="XM_034336599.2:1356..2132"
                     /db_xref="GeneID:117609837"
ORIGIN      
        1 melaikkeel pcnelmktle rnvailkien hynlkigyel etkltwynnv srgladklds
       61 lwklaeahhs sgneikqfad qishswtsvn rqicdkyski qmasrtlhgv plsvildkvk
      121 keiillkksl kinqsiynke yirkgyqlit eseelihslk kcdsklqqyl sitnitphli
      181 kletaidkyv tnvellpaqk sflsdlsifs lviklltgel ldheyidpny vlmenvpkkp
      241 vfiikntkrk tinsyypt
//
LOCUS       XP_034191786             722 aa            linear   INV 03-SEP-2025
DEFINITION  heat shock protein 90 [Osmia lignaria lignaria].
ACCESSION   XP_034191786
VERSION     XP_034191786.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335895.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..722
                     /product="heat shock protein 90"
                     /calculated_mol_wt=83137
     Region          14..722
                     /region_name="HSP90"
                     /note="Hsp90 protein; cl46693"
                     /db_xref="CDD:481033"
     CDS             1..722
                     /gene="Hsp90"
                     /coded_by="XM_034335895.2:194..2362"
                     /db_xref="GeneID:117609470"
ORIGIN      
        1 mpddvmmsea gevetfafqa eiaqlmslii ntfysnkeif irelisnasd aldkiryesl
       61 tdpskldtck elfikiipnk ndrtltimds gigmtkadlv nnlgtiaksg tkafmealqa
      121 gadismigqf gvgfysaylv adkvvviskh nddeqyvwes saggsftvrp dngerigrgt
      181 kiilhikedq teyleeskik eivkkhsqfi gypiklvvek erdkelsede eeepakeget
      241 edekpkiedv gedededkpk dekkkkkkti kekytedeel nktkpiwtrn pdditqeeyg
      301 efyksltndw edhlavkhfs vegqlefral lfiprrapfd lfenkkrknn iklyvrrvfi
      361 mdncedlipe ylnfikgvvd sedlplnisr emlqqnkilk virknlvkkc lelfeelaed
      421 kenykkcyeq fsknlklgih edsqnrkkla ellryhtsas gdemcslkdy vgrmkenqkh
      481 iyyitgeske qvanssfver vkkrgfeviy mtepideyvv qqlkefdgkq lvsvtkegle
      541 lpedeedkkk readkvkfen lckvmkdild kkvekvvvsn rlvdspcciv tsqygwtanm
      601 erimkaqalr dtstmgymaa kkhleinpdh pimenlrqka eadkhdksvk dlvmllfeta
      661 llssgfaled pqvhasriyr miklglgfde edappvedek mdtevppleg daeeasrmee
      721 vd
//
LOCUS       XP_034182781           24068 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034182781
VERSION     XP_034182781.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182781.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24068
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..24068
                     /product="sallimus isoform X1"
                     /calculated_mol_wt=2719445
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16500..>16652
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22306..22358
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22311,22313,22319..22320,22337..22339,22352,22354,
                     22356..22357)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22394..22484
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22411..22415
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22424..22428
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22450..22454
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22464..22469
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22477..22480
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22518..22611
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22535..22539
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22548..22552
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22573..22581
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22591..22596
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22604..22607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22619..22705
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22636..22640
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22649..22653
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22671..22675
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22685..22690
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22698..22701
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22739..22829
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22756..22760
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22769..22773
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22794..22798
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22809..22814
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22822..22825
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22842..22919
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22852..22856
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22863..22867
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22888..22892
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22902..22907
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22923..23008
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22940..22944
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22952..22956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22978..22982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22992..22997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23001..23004
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23013..23097
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23030..23034
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23042..23046
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23067..23071
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23081..23086
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23090..23093
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23203..23283
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23211..23215
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23224..23228
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23249..23253
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23263..23268
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23276..23279
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23287..23378
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23287,23352,23367)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23368..23369,23371..23372)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23389..23475
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23406..23410
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23419..23423
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23441..23445
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23455..23460
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23468..23471
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23499..23569
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23499..23502
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23511..23515
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23535..23539
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23549..23554
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23562..23565
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23573..23665
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23573,23639,23654)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23643..23958
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23655..23656,23658..23659)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..24068
                     /gene="sls"
                     /coded_by="XM_034326890.2:755..72961"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    13501 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    13561 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    13621 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    13681 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    13741 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    13801 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    13861 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    13921 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    13981 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    14041 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    14101 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    14161 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    14221 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    14281 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    14341 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    14401 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    14461 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    14521 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    14581 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    14641 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    14701 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    14761 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    14821 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    14881 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    14941 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    15001 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    15061 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    15121 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    15181 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    15241 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    15301 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    15361 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    15421 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    15481 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    15541 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    15601 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    15661 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    15721 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    15781 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    15841 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    15901 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    15961 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    16021 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    16081 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    16141 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    16201 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    16261 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    16321 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    16381 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    16441 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    16501 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    16561 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    16621 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    16681 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    16741 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    16801 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivetteapaq qhhititqht
    16861 tkhdlpqqqs iesdteviee yttkfrrgst edataavktk ktivrkkpkt tgeedivvvi
    16921 eeelddikpk ipsipkkqfm pieevttvsd veeivsarid eaeeivpkee ekvevtitev
    16981 qdtdkkkkvt kkqfvrrrgk trqvteeitv eeegkapett vvtkpveiie edieerpkie
    17041 keeyvveevp eektiplkpr kkltptvqqv eeqvtvteei tkegkprktt kkkvitrkgk
    17101 eqqvtevvtv eeqgkapits vtegpveevv eeiiqklpap kyakpsevee vreqvtvtee
    17161 vtrlgkpkkt tkkkiikrkg keqqvtevvt veeegkaptt tvtegpveev vieeikelpi
    17221 pefvkpteve evreqvtvte eltkmgkpkk tikkkiikrk gkeqqvtevv tveeqgkapv
    17281 ttvkegpvee vveetitpll apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    17341 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    17401 tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt vtegpveevv detvkalpap
    17461 eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    17521 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    17581 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    17641 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    17701 reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv tevvtveeqg kapvttvneg
    17761 pveevvdetv kalpapeyae pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    17821 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    17881 kktikkkiik kkgkeqqvte lvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    17941 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp
    18001 veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv
    18061 tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk
    18121 kttkkkiikr kgkeqqvtei vtveeegkap kttvmegpve evvieeiqel pipefvkpte
    18181 veevreqvtv teevtrlgkp kkttkkkiik rkgkeqqvte ivtveeqgka pvttvtegpv
    18241 eevvdetiks lpapeyakps eveevreqvt vtqevtklgk pkkttkkkiv krkgkeqqvt
    18301 eivtveeegk apkttvtegp veevveeevk pllapefvkp seveevheqv tvtqevtreg
    18361 kpmktikkki ikkkgkaqqv tevvtveeeg kvpvttvkeg pveevvdetv kalpapkyak
    18421 pseveevreq vtvtqeitkl gkpkkttkrk vikrkgkeqq vtevvtveee gkapettvte
    18481 gpveevleee ikplpgpefv kpseveevre qvtvteevtr egkpkktikk kiikkkgkeq
    18541 qvteivtvee qgkapvtsvt egpveevvde tikqlpapky akpseveevr eqvtvtqemt
    18601 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttm tegpveevve eeikplpale
    18661 fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk eqqitevvtv eeqgkapvtt
    18721 vtkapveeiv detikelpal efakpsevee vreqvtvtke vtkegkpkkt ikrkvikkkg
    18781 keqqvteivt veeegkapkt titegpveev veeeikplpa lefvkpseve evreqvtvte
    18841 evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv ttvqegpvee videtikslp
    18901 apeyakpsev eeireqvtvt eeltklgkpk kttkkkitkr kgpeqqitei vtveeegkap
    18961 vttvtegpve evvkevlkpl pvpkvvkpse veeireqvtv teemtkegkp kkiikkkvik
    19021 rkgreqtvqe vvtveekgka pvttvtvgpt eeiveetvkt lpapeyakps eveevreqvt
    19081 vtqevtkegk pkkttkkkvi krkgkeqqvt evvtveeqgk apvttvtegp veevveellk
    19141 pliapeyvkp seveevreqv tvteeltkeg kpkkvikkkv ikrrgreqqv kevvtveeqg
    19201 kapvttvteg pteeiveeti kalpapedak pseveevreq vtvtqevtke gkpkktikkk
    19261 vtkrkgkeqq vtevvtveek gkapvttvte gpveeiveet vkalpapelv kptevtevpe
    19321 eltvtevvtk egktkkivkk kvpkpkkegi eapeeapeev pveetkkiik lqrlkvskfe
    19381 stkldiqets lgkpqfaqik lrktpvakrp skkdekfpri llrsriiaie wppspknlkv
    19441 dtfepielqn gilsrnvdea aalpkpkkkk ikriekeiak lekldqefee lkkelplekl
    19501 eepileekpe rkvkkkkkpe dkakegvkpq lmrisikeqk pikpkiaepa tplfaqiklk
    19561 kapakpkkkp eeevskfpki llrsritahe wppsvkypai svlepnyiqn gilsrtmeea
    19621 lklkkvkhkk mklpekelte lekldqefee lkkvplekve eitpyerkpk pekpeeekpk
    19681 klvigkgkrr paeeedletv qlkkipekip sapeeaekvp kkkdevdkpk kekeekeype
    19741 lkpfepydie apeveleere kpeypepekp kekkvpgkkp kkerktpaqe teavpivpgv
    19801 pkpkepeeee eikrriperp ppteepekik lkpwkkgekp eeeekkpedy ikfvpkdddi
    19861 vetvevtttv eetpdkkqrk ikkkktitkp kdekpkiiee ttieeegfep vvtveefdvd
    19921 kyeavpvesv ekaapveeik eeivtseiit eegtkktvrk krvtkkregk ervteevtve
    19981 eegkepitat ielhedtise iheqakhkrp tkpkadekla edvtektveq qeiieevkev
    20041 kptkkvikke kpvkakdevt eevveeiiie epekqeakpi veeeetviit ehdvrkapkk
    20101 kkevvikeee eifetietpt hkiirkkirp mkkgekevvv eeiiqkpree tvemeeqqiv
    20161 evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk akptveeekk kvkvtkvdtk
    20221 kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii eeviqktiee akameeqeiv
    20281 evietptkkv ikkktttiee gkapeekvee iviekpkevi eeeeeiieti etptkrivrk
    20341 kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv ikkkkatike gkapeeevee
    20401 iviekpikek akkkilpkeg veitevitet sveelieeel kpeeakpsee veeekvkite
    20461 vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp tvtekegiev tevisevape
    20521 klpeeakpkk kkateikete ekvitkkvkv keapkeavpg eeeqpaeevd elvpekpkkt
    20581 kakkevtpqd sivttevvpi tkeeelevek peeekatvve ekeekakvee kkvtvkkapk
    20641 kklkpteaee eqveevveei tpeepkkrka kkavipkeei qttevipgit aeeipeeekp
    20701 eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge vieekpkvke ipeekpkeev
    20761 pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee vkpeeekpev kpeeekpkee
    20821 kpkkkkpaek kpkiseeerp edkveeipek kpeevktree iipkdtvqtt evipetvaee
    20881 iptkkpkerk avpveekeek veitevpvek apekkeekvp eekpteaape kpkeakvmkk
    20941 kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke kkplpeslqk lvpqkierke
    21001 mkptklkyse paetvqfasi klkkvavpkk keadetkfpk imlrsrmtfv gdyppelqip
    21061 kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev tqlekldlef eelkkvplek
    21121 iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe kvklkkvpek kpeeveeplk
    21181 kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek yepeeyekpe epapepvpkp
    21241 yekepkkkpd qeveqiplkk gkpkspepde epevkfript kekpeeepeq itlkpwkkek
    21301 peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk ekpkeekeee vtvkkpeeek
    21361 peevaeevtl kkepekpvee apaevtikkp vveekeveee etitevilkk kpekpeevve
    21421 eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi kkpkkksipe iteevteeit
    21481 lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv eekkeeevpe evtlkpkkpe
    21541 ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee aadeitlkkp vvvekeeeei
    21601 teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape evteeiaikk pkkkkpvkee
    21661 aadevtikkp vveekkeeet peevtlkprk pkekpvpeev keevtlkkpv peekekvpee
    21721 vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit eeitlgkkkk pekkpvpeei
    21781 teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea adevtikkpv vvekeeeeap
    21841 aeitlkrrpk ekpveeitee vteeitikkp tpeekekape evsekvevkk pkkkkpvvee
    21901 aadevtikkv vveekkeeea peevtlkprp kkksvteeev aevtltkpkp ieeekkpees
    21961 eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti rkkkkpekkp kvteeveeta
    22021 vtikkarape kpteeeeitt dvqikkkkpq kkveevaade ltikkieeve qpeeeeevqe
    22081 ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei tetenvtfrp rttktkedve
    22141 qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet iriiqevedd sgpiieeiie
    22201 sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie egdvklklkr erkysieead
    22261 eslalklksk rrvstydeee atlsitreed iseeveieyv vrdgdtmfsi csyvaetdea
    22321 inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln evhytlylqr klhekidklp
    22381 vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv prpqitwfrq taiikpspdf
    22441 qmyydddnva tliirevfpe dagtftcvak naagfasstt eliveaplsd hgsdltgpsr
    22501 kslsressla dilegipptf srkpkakyvd egedvilecr lvavpepeit wyykdtqitt
    22561 keniiiates dmhmycsvik itkvqkkqeg kytivarnre geatieipmk vktgkseppe
    22621 ileplqsyvv regetvvlst qivgnpapkv twfkngkplk gltpkqdghv ntltliqpqv
    22681 sdtgeysvva tndlgtaetk atltveinlk pslainfqqh tfehfsvtke ipsgapeppl
    22741 fterfqeltv pekgtfklva kvtgnpvpev twlrnnkple kspnitetyd genivleirn
    22801 ansevdtgdy kcvasnpvgk ashgarvtvd vdkvtftktl ekevvvdeyk tleltcetsh
    22861 tvstkwwhnd keisgmdhre iieegrvhkl vikktnptde gtykctvknq stssnvtvka
    22921 tkpefvkklq dcevkerdva ileveitsqt vdvkwykdge qlgpgkekle fvkegtvrkl
    22981 lirstsvhde geytcslldq ectaevtvie lppeiitkmq dvtiargera tfdieltkgd
    23041 alvrwfkdgq elqfsehvrl sidgkrqklk iydtesedag vyscevgqqk ssakltveep
    23101 avefitklpd vtlvplnsda vfvielsqdv pvtwmrktqv ikespkytii degkvkkliv
    23161 kkcttedise ytamvanvkt ssrlrvevie tppkispdtp rkykvrkded vdivvkfsgt
    23221 pkpedewtvn grvvtkskra iptideesav ltirkiqeed vgdytlklvn svgetsiein
    23281 vvivqvpsap gapeplettn dsvtlhwkkp dsdgnspive yilehqekte ttwtkiteti
    23341 tetthkvtkl ttnkeytfrv tavneagpge tsptspyvki skpstaeppv ileplksivi
    23401 glgetvtlsc viggtpapei twlrndeife dtsityenhi tkytirette tssakftvka
    23461 knnvgsaett celkiqespk iiydenltnq klpmngqwki eiqitgfpkp evtwtkngke
    23521 vdekrvsihr eertstvsis slvredtaty makaenqags ssvdlhlkvi dkpskpqgpv
    23581 vfkeirqdrv tlewrppadd ggielekyti ekcepgktwv kvvdidkeve sfcvhklqqn
    23641 aeykfriiar navgaseple sdtvkmrtsf eppgaprgpl evsgmtktsf tikwqppend
    23701 ggtpitdyii eikeeskktw qkigstkhet thfsvsdlkt dvpynfrita knsvgsgppy
    23761 vaeepiapgr rikitkltes smyallgifp ptkivisktp psspqhvhvt nitsksatls
    23821 wsppastggt eltgyviekr pligkgarwt kvvtldattl qycvenlkes eflfrifaen
    23881 nqglsvpans epvtlkthas vpspptaple irqiaantvv iswgrpdsdg gaplegykva
    23941 irdtkrtmwm evarvnteiq klnirdlqen hmylirifar neiglsdple seepfkiipa
    24001 seltvvepia eatekgetas isfstentss wlrehnmdad ihsyararll rqdeyffriw
    24061 hyakklfe
//
LOCUS       XP_034182790           24067 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182790
VERSION     XP_034182790.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326899.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182790.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24067
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..24067
                     /product="sallimus isoform X2"
                     /calculated_mol_wt=2719317
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16500..>16652
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22305..22357
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22310,22312,22318..22319,22336..22338,22351,22353,
                     22355..22356)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22393..22483
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22410..22414
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22423..22427
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22449..22453
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22463..22468
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22476..22479
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22517..22610
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22534..22538
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22547..22551
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22572..22580
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22590..22595
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22603..22606
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22618..22704
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22635..22639
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22648..22652
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22670..22674
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22684..22689
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22697..22700
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22738..22828
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22755..22759
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22768..22772
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22793..22797
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22808..22813
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22821..22824
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22841..22918
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22851..22855
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22862..22866
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22887..22891
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22901..22906
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22922..23007
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22939..22943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22951..22955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22977..22981
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22991..22996
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23000..23003
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23012..23096
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23029..23033
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23041..23045
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23066..23070
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23080..23085
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23089..23092
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23202..23282
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23210..23214
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23223..23227
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23248..23252
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23262..23267
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23275..23278
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23286..23377
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23286,23351,23366)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23367..23368,23370..23371)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23388..23474
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23405..23409
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23418..23422
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23440..23444
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23454..23459
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23467..23470
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23498..23568
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23498..23501
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23510..23514
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23534..23538
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23548..23553
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23561..23564
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23572..23664
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23572,23638,23653)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23642..23957
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23654..23655,23657..23658)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..24067
                     /gene="sls"
                     /coded_by="XM_034326899.2:755..72958"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    13501 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    13561 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    13621 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    13681 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    13741 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    13801 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    13861 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    13921 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    13981 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    14041 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    14101 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    14161 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    14221 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    14281 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    14341 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    14401 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    14461 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    14521 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    14581 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    14641 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    14701 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    14761 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    14821 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    14881 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    14941 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    15001 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    15061 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    15121 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    15181 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    15241 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    15301 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    15361 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    15421 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    15481 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    15541 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    15601 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    15661 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    15721 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    15781 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    15841 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    15901 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    15961 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    16021 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    16081 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    16141 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    16201 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    16261 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    16321 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    16381 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    16441 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    16501 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    16561 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    16621 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    16681 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    16741 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    16801 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivetteapaq qhhititqht
    16861 tkhdlpqqqs iesdteviee yttkfrrgst edataavktk ktivrkkpkt tgeedivvvi
    16921 eeelddikpk ipsipkkqfm pieevttvsd veeivsarid eaeeivpkee ekvevtitev
    16981 qdtdkkkkvt kkqfvrrrgk trqvteeitv eeegkapett vvtkpveiie edieerpkie
    17041 keeyvveevp eektiplkpr kkltptvqqv eeqvtvteei tkegkprktt kkkvitrkgk
    17101 eqqvtevvtv eeqgkapits vtegpveevv eeiiqklpap kyakpsevee vreqvtvtee
    17161 vtrlgkpkkt tkkkiikrkg keqqvtevvt veeegkaptt tvtegpveev vieeikelpi
    17221 pefvkpteve evreqvtvte eltkmgkpkk tikkkiikrk gkeqqvtevv tveeqgkapv
    17281 ttvkegpvee vveetitpll apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    17341 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    17401 tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt vtegpveevv detvkalpap
    17461 eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    17521 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    17581 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    17641 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    17701 reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv tevvtveeqg kapvttvneg
    17761 pveevvdetv kalpapeyae pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    17821 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    17881 kktikkkiik kkgkeqqvte lvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    17941 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp
    18001 veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv
    18061 tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk
    18121 kttkkkiikr kgkeqqvtei vtveeegkap kttvmegpve evvieeiqel pipefvkpte
    18181 veevreqvtv teevtrlgkp kkttkkkiik rkgkeqqvte ivtveeqgka pvttvtegpv
    18241 eevvdetiks lpapeyakps eveevreqvt vtqevtklgk pkkttkkkiv krkgkeqqvt
    18301 eivtveeegk apkttvtegp veevveeevk pllapefvkp seveevheqv tvtqevtreg
    18361 kpmktikkki ikkkgkaqqv tevvtveeeg kvpvttvkeg pveevvdetv kalpapkyak
    18421 pseveevreq vtvtqeitkl gkpkkttkrk vikrkgkeqq vtevvtveee gkapettvte
    18481 gpveevleee ikplpgpefv kpseveevre qvtvteevtr egkpkktikk kiikkkgkeq
    18541 qvteivtvee qgkapvtsvt egpveevvde tikqlpapky akpseveevr eqvtvtqemt
    18601 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttm tegpveevve eeikplpale
    18661 fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk eqqitevvtv eeqgkapvtt
    18721 vtkapveeiv detikelpal efakpsevee vreqvtvtke vtkegkpkkt ikrkvikkkg
    18781 keqqvteivt veeegkapkt titegpveev veeeikplpa lefvkpseve evreqvtvte
    18841 evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv ttvqegpvee videtikslp
    18901 apeyakpsev eeireqvtvt eeltklgkpk kttkkkitkr kgpeqqitei vtveeegkap
    18961 vttvtegpve evvkevlkpl pvpkvvkpse veeireqvtv teemtkegkp kkiikkkvik
    19021 rkgreqtvqe vvtveekgka pvttvtvgpt eeiveetvkt lpapeyakps eveevreqvt
    19081 vtqevtkegk pkkttkkkvi krkgkeqqvt evvtveeqgk apvttvtegp veevveellk
    19141 pliapeyvkp seveevreqv tvteeltkeg kpkkvikkkv ikrrgreqqv kevvtveeqg
    19201 kapvttvteg pteeiveeti kalpapedak pseveevreq vtvtqevtke gkpkktikkk
    19261 vtkrkgkeqq vtevvtveek gkapvttvte gpveeiveet vkalpapelv kptevtevpe
    19321 eltvtevvtk egktkkivkk kvpkpkkegi eapeeapeev pveetkkiik lqrlkvskfe
    19381 stkldiqets lgkpqfaqik lrktpvakrp skkdekfpri llrsriiaie wppspknlkv
    19441 dtfepielqn gilsrnvdea aalpkpkkkk ikriekeiak lekldqefee lkkelplekl
    19501 eepileekpe rkvkkkkkpe dkakegvkpq lmrisikeqk pikpkiaepa tplfaqiklk
    19561 kapakpkkkp eeevskfpki llrsritahe wppsvkypai svlepnyiqn gilsrtmeea
    19621 lklkkvkhkk mklpekelte lekldqefee lkkvplekve eitpyerkpk pekpeeekpk
    19681 klvigkgkrr paeeedletv qlkkipekip sapeeaekvp kkkdevdkpk kekeekeype
    19741 lkpfepydie apeveleere kpeypepekp kekkvpgkkp kkerktpaqe teavpivpgv
    19801 pkpkepeeee eikrriperp ppteepekik lkpwkkgekp eeeekkpedy ikfvpkdddi
    19861 vetvevtttv eetpdkkqrk ikkkktitkp kdekpkiiee ttieeegfep vvtveefdvd
    19921 kyeavpvesv ekaapveeik eeivtseiit eegtkktvrk krvtkkregk ervteevtve
    19981 eegkepitat ielhedtise iheqakhkrp tkpkadekla edvtektveq eiieevkevk
    20041 ptkkvikkek pvkakdevte evveeiiiee pekqeakpiv eeeetviite hdvrkapkkk
    20101 kevvikeeee ifetietpth kiirkkirpm kkgekevvve eiiqkpreet vemeeqqive
    20161 vvetptkkiv kkkkevvkdd gvpeelveei iiekpqkkka kptveeekkk vkvtkvdtkk
    20221 apekpeevve eeeeiietie tptkkiirkk pkdkkdkiie eviqktieea kameeqeive
    20281 vietptkkvi kkktttieeg kapeekveei viekpkevie eeeeiietie tptkrivrkk
    20341 pkdkkdkive eviqktieea ktmkeqeiie vietptktvi kkkkatikeg kapeeeveei
    20401 viekpikeka kkkilpkegv eitevitets veelieeelk peeakpseev eeekvkitev
    20461 aikkvpkkkk pskaveaeek eevveeikpe kpkeekakpt vtekegievt evisevapek
    20521 lpeeakpkkk kateiketee kvitkkvkvk eapkeavpge eeqpaeevde lvpekpkktk
    20581 akkevtpqds ivttevvpit keeelevekp eeekatvvee keekakveek kvtvkkapkk
    20641 klkpteaeee qveevveeit peepkkrkak kavipkeeiq ttevipgita eeipeeekpe
    20701 eekavpkeee kveeeakvte vsvkkkerkk kekpvtngev ieekpkvkei peekpkeevp
    20761 qvpeekekpe eekpkevkpk ekkpkeekpk kpieekpeev kpeeekpevk peeekpkeek
    20821 pkkkkpaekk pkiseeerpe dkveeipekk peevktreei ipkdtvqtte vipetvaeei
    20881 ptkkpkerka vpveekeekv eitevpveka pekkeekvpe ekpteaapek pkeakvmkkk
    20941 kkkpakkdei eewvepeyer pvlepmpeki vwepkkkkek kplpeslqkl vpqkierkem
    21001 kptklkysep aetvqfasik lkkvavpkkk eadetkfpki mlrsrmtfvg dyppelqipk
    21061 ityleenpvq tgilsrntee alkvlkrkvk kvklsekevt qlekldlefe elkkvpleki
    21121 edksvyerqp kkpaekpeep kkvpigkgkv peeekvepek vklkkvpekk peeveeplkk
    21181 pkkpepeeev kpekkkkpkv klehdelkpl dfdrpeleky epeeyekpee papepvpkpy
    21241 ekepkkkpdq eveqiplkkg kpkspepdee pevkfriptk ekpeeepeqi tlkpwkkekp
    21301 eeekekeipk keeekvpkpp keepedvtvq rkaktkkpke kpkeekeeev tvkkpeeekp
    21361 eevaeevtlk kepekpveea paevtikkpv veekeveeee titevilkkk pekpeevvee
    21421 avvkkpkkkk pvkeeevvee vtvtkvvtee ekeeekvvik kpkkksipei teevteeitl
    21481 kkpeekapee vteeikkpkk kkpvkeevae eitikkpvve ekkeeevpee vtlkpkkpee
    21541 kpvieevtee itlkkpvpee vteevaikkp kkkkpveeea adeitlkkpv vvekeeeeit
    21601 eevtlqkkkk pekkpvpeei seeitlkkpv pkeeekapee vteeiaikkp kkkkpvkeea
    21661 adevtikkpv veekkeeetp eevtlkprkp kekpvpeevk eevtlkkpvp eekekvpeev
    21721 teeiaikkpk kkkpveeeaa devtikkpvv vekeeeeite eitlgkkkkp ekkpvpeeit
    21781 eeitlkkpvp keeekapeev tekievkkpk kkkpveeeaa devtikkpvv vekeeeeapa
    21841 eitlkrrpke kpveeiteev teeitikkpt peekekapee vsekvevkkp kkkkpvveea
    21901 adevtikkvv veekkeeeap eevtlkprpk kksvteeeva evtltkpkpi eeekkpeese
    21961 ltikkkpkkk pvieeeaaev tikkpvpvep deapeeitir kkkkpekkpk vteeveetav
    22021 tikkarapek pteeeeittd vqikkkkpqk kveevaadel tikkieeveq peeeeevqef
    22081 tfkrkppklp pkpieeiyed vtlrklrpkr ksrpdikeit etenvtfrpr ttktkedveq
    22141 efkislntye eedismsgkv rlkpkkkplt yseetgeeti riiqevedds gpiieeiies
    22201 deegkdeysi eeldtdemrl plrrkkkkvp kpykvediee gdvklklkre rkysieeade
    22261 slalklkskr rvstydeeea tlsitreedi seeveieyvv rdgdtmfsic syvaetdeai
    22321 nlvegervyv idhtnqdwwf vkkhlteekg wvpaqyllne vhytlylqrk lhekidklpv
    22381 fekpapgdks sapkfveklq pihtpdgyti qfecqvegvp rpqitwfrqt aiikpspdfq
    22441 myydddnvat liirevfped agtftcvakn aagfasstte liveaplsdh gsdltgpsrk
    22501 slsresslad ilegipptfs rkpkakyvde gedvilecrl vavpepeitw yykdtqittk
    22561 eniiiatesd mhmycsviki tkvqkkqegk ytivarnreg eatieipmkv ktgkseppei
    22621 leplqsyvvr egetvvlstq ivgnpapkvt wfkngkplkg ltpkqdghvn tltliqpqvs
    22681 dtgeysvvat ndlgtaetka tltveinlkp slainfqqht fehfsvtkei psgapepplf
    22741 terfqeltvp ekgtfklvak vtgnpvpevt wlrnnkplek spnitetydg enivleirna
    22801 nsevdtgdyk cvasnpvgka shgarvtvdv dkvtftktle kevvvdeykt leltcetsht
    22861 vstkwwhndk eisgmdhrei ieegrvhklv ikktnptdeg tykctvknqs tssnvtvkat
    22921 kpefvkklqd cevkerdvai leveitsqtv dvkwykdgeq lgpgkeklef vkegtvrkll
    22981 irstsvhdeg eytcslldqe ctaevtviel ppeiitkmqd vtiargerat fdieltkgda
    23041 lvrwfkdgqe lqfsehvrls idgkrqklki ydtesedagv yscevgqqks sakltveepa
    23101 vefitklpdv tlvplnsdav fvielsqdvp vtwmrktqvi kespkytiid egkvkklivk
    23161 kcttedisey tamvanvkts srlrveviet ppkispdtpr kykvrkdedv divvkfsgtp
    23221 kpedewtvng rvvtkskrai ptideesavl tirkiqeedv gdytlklvns vgetsieinv
    23281 vivqvpsapg apeplettnd svtlhwkkpd sdgnspivey ilehqektet twtkitetit
    23341 etthkvtklt tnkeytfrvt avneagpget sptspyvkis kpstaeppvi leplksivig
    23401 lgetvtlscv iggtpapeit wlrndeifed tsityenhit kytirettet ssakftvkak
    23461 nnvgsaettc elkiqespki iydenltnqk lpmngqwkie iqitgfpkpe vtwtkngkev
    23521 dekrvsihre ertstvsiss lvredtatym akaenqagss svdlhlkvid kpskpqgpvv
    23581 fkeirqdrvt lewrppaddg gielekytie kcepgktwvk vvdidkeves fcvhklqqna
    23641 eykfriiarn avgaseples dtvkmrtsfe ppgaprgple vsgmtktsft ikwqppendg
    23701 gtpitdyiie ikeeskktwq kigstkhett hfsvsdlktd vpynfritak nsvgsgppyv
    23761 aeepiapgrr ikitkltess myallgifpp tkivisktpp sspqhvhvtn itsksatlsw
    23821 sppastggte ltgyviekrp ligkgarwtk vvtldattlq ycvenlkese flfrifaenn
    23881 qglsvpanse pvtlkthasv pspptaplei rqiaantvvi swgrpdsdgg aplegykvai
    23941 rdtkrtmwme varvnteiqk lnirdlqenh mylirifarn eiglsdples eepfkiipas
    24001 eltvvepiae atekgetasi sfstentssw lrehnmdadi hsyararllr qdeyffriwh
    24061 yakklfe
//
LOCUS       XP_034182800           24067 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034182800
VERSION     XP_034182800.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326909.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182800.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24067
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..24067
                     /product="sallimus isoform X3"
                     /calculated_mol_wt=2719388
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3177..3266
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3194..3198
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3207..3211
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3229..3236
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3246..3251
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3259..3262
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3273..3363
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3290..3294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3303..3307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3329..3333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3343..3348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3356..3359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3998..4269
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4984..5073
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5001..5005
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5014..5018
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5039..5043
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5053..5058
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5066..5069
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5230..5317
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5247..5251
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5260..5264
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5287..5291
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5301..5306
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5427..5516
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5444..5448
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5457..5461
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5482..5486
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5496..5501
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5509..5512
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5552..5642
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5569..5573
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5582..5586
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5608..5612
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5622..5627
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5635..5638
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5651..5741
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5668..5672
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5681..5685
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5707..5711
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5721..5726
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5734..5737
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5798..5889
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5815..5819
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5830..5834
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5856..5859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5869..5874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5882..5885
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5931..6023
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5949..5953
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5964..5968
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5989..5993
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6003..6008
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6016..6019
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6070..6162
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6088..6092
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6103..6107
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6128..6132
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6142..6147
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6155..6158
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6204..6296
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6222..6226
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6237..6241
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6263..6267
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6276..6281
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6289..6292
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6338..6430
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6356..6360
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6371..6375
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6396..6400
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6410..6415
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6423..6426
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6472..6563
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6489..6493
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6504..6508
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6530..6533
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6543..6548
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6556..6559
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6605..6695
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6623..6627
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6638..6642
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6663..6667
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6677..6682
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6690..6693
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6739..6830
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6756..6760
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6767..6775
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6796..6800
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6810..6815
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6823..6826
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6872..6963
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6889..6893
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6904..6908
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6929..6933
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6943..6948
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6956..6959
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7005..7097
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7023..7027
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7038..7042
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7063..7067
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7077..7082
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7090..7093
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7139..7231
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7157..7161
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7170..7176
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7197..7201
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7211..7216
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7224..7227
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7271..7363
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7289..7293
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7302..7308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7329..7333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7343..7348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7356..7359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7404..7496
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7422..7426
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7435..7441
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7462..7466
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7476..7481
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7489..7492
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7538..7630
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7556..7560
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7571..7575
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7596..7600
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7610..7615
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7623..7626
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7670..7762
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7703..7707
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7728..7732
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7742..7747
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7804..7896
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7822..7826
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7837..7841
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7862..7866
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7876..7881
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7889..7892
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7936..8026
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7953..7957
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7968..7972
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7993..7997
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8007..8012
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8020..8023
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8103..8163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8107..8111
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8132..8136
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8146..8151
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8160..8163
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8211..8302
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8244..8248
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8269..8273
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8283..8288
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8312..8403
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8330..8334
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8345..8349
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8367..8374
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8384..8389
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8397..8400
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8445..8536
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8463..8467
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8478..8482
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8500..8504
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8517..8522
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8530..8533
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8583..8674
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8616..8620
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8641..8645
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8655..8660
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8721..8812
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8738..8742
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8753..8757
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8778..8782
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8792..8797
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8805..8808
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8858..8948
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8875..8879
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8890..8894
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8915..8919
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8929..8934
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8942..8945
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8991..9080
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9008..9012
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9023..9027
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9048..9052
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9062..9067
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9075..9078
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9134..9224
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9152..9156
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9167..9171
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9189..9193
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9206..9211
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9219..9222
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9272..9360
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9289..9293
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9302..9306
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9326..9330
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9340..9345
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9353..9356
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9595..9685
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9612..9616
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9625..9629
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9650..9654
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9664..9669
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9677..9680
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9699..9788
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9716..9720
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9729..9733
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9754..9758
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9768..9773
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9781..9784
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9809..9898
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9826..9830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9839..9843
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9864..9868
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9878..9883
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9891..9894
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9910..9997
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9924..9928
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9938..9942
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9963..9967
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9977..9982
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9990..9993
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10114..10767
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10820..10973
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12248..12337
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12265..12269
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12278..12282
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12303..12307
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12317..12322
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12347..12438
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12364..12368
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12377..12381
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12404..12408
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12418..12423
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12431..12434
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12453..12542
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12470..12474
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12483..12487
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12508..12512
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12522..12527
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12537..12540
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12555..12641
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12572..12576
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12585..12589
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12610..12614
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12624..12629
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12637..12640
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12658..12748
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12675..12679
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12688..12692
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12714..12718
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12728..12733
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12741..12744
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12796..12882
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12815..12819
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12828..12832
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12854..12858
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12868..12873
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12920..13011
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12937..12941
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12950..12954
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12977..12981
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12991..12996
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13004..13007
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16499..>16651
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22305..22357
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22310,22312,22318..22319,22336..22338,22351,22353,
                     22355..22356)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22393..22483
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22410..22414
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22423..22427
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22449..22453
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22463..22468
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22476..22479
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22517..22610
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22534..22538
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22547..22551
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22572..22580
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22590..22595
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22603..22606
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22618..22704
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22635..22639
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22648..22652
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22670..22674
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22684..22689
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22697..22700
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22738..22828
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22755..22759
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22768..22772
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22793..22797
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22808..22813
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22821..22824
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22841..22918
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22851..22855
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22862..22866
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22887..22891
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22901..22906
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22922..23007
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22939..22943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22951..22955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22977..22981
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22991..22996
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23000..23003
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23012..23096
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23029..23033
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23041..23045
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23066..23070
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23080..23085
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23089..23092
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23202..23282
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23210..23214
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23223..23227
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23248..23252
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23262..23267
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23275..23278
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23286..23377
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23286,23351,23366)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23367..23368,23370..23371)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23388..23474
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23405..23409
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23418..23422
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23440..23444
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23454..23459
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23467..23470
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23498..23568
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23498..23501
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23510..23514
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23534..23538
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23548..23553
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23561..23564
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23572..23664
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23572,23638,23653)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23642..23957
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23654..23655,23657..23658)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..24067
                     /gene="sls"
                     /coded_by="XM_034326909.2:755..72958"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei gsevqrdkke vveekvyelv eripgdemha ssssdkhfsa ekseiggenk
     3061 vltkmsetit esstthktlk keyisssmss stgapgqept svcvkstvhs teqsssadga
     3121 ppvvqsykve ehekiiqdqp geirqertvv vsqdeegikr dikatqipkp vrkstaprfv
     3181 spvtgmivdq gsdivlegii dgfpqpvviw skngqelktk dsmtityshn hvrlelknvn
     3241 vkdagrytct asnevgsass tadlvvkkti fppvfgrrlq aqlvkrgdrv nmeveitgtp
     3301 eptvswfkdd ipikerppel rikqqgncym limekaakeh agkymvratn aggeaqsiad
     3361 favfeptpdt mvevhktviy dvqdkklmqs dekkaevpss khltseqvst tkiqpaatik
     3421 ttptamstsm aqmsrtveki kepdtelcrs ekisstvesh qmerkseqky hmklehkmps
     3481 ivepktegkt vikevireev rssepkstli eekienenie tsriakkdal sffesmtret
     3541 etipkgpkem iklvddadgh evkvgkltkn yekstsfqev kkpevkppel lttkkavhei
     3601 ftkleqgsip srgidnklfd fpyepykptm etkktvveet issgssslqs smtmskmesk
     3661 reskmeskss ellsegfnlv pepppeigym pkpeevkkkr pevsikakql qesfdktlsp
     3721 idapiggvki fpspspkile tpkvappsfs mppppfqlek keiveevcik kdvhekrdpq
     3781 plvrpispsr pwssssdvet rsqmstdlse yrchsaassh qeilrssspr psadglamek
     3841 swakkcadst rkswppqedt pikykqewqm pgedyktssk efkreveetp eggikktsme
     3901 ssstterrsw sskqetmdkm skrapsppki qpiiynaeti kvdhtvnkie ekslyekyis
     3961 ecdvkksqks ekleeysskk wtgaedlkap glvrsletpk kptpplyrpv epiptdqiil
     4021 epgpppeigy ipapvvrekk ieqiektlev slehkpakip pggirtippa pkkeeppalp
     4081 pkdvritppp lpakqptpve plepfpfkps psptiktpkv avpaptpskf vkgsfgvesd
     4141 yesdmdshmk akwrpyesdn eepryrrvqp pvpkqprprs tepeplppsk fevpsestgl
     4201 prpvltkeqq ekmykktttf krqeketkqq kyqvvqpppv elkpgsppiy vqpatksptp
     4261 ksppakkpes pkfkvktfqq esgymadtde plqqkssvas lsksfgkqes sssmsshmes
     4321 rtsysesrse yfesksydsr qqqqkkesfa psapssvkqp pqqrssfvek sygssavtss
     4381 prfmsaketl yttdqshkkf eapkkilpps pspsrfvkse fresdyesdy dgripplwkp
     4441 rgyesddqsf rtvkpnlagp gkpkvqredt srlieeastr aqkaktfekr eervatkpkt
     4501 vllpgsppei ayappqpqpt yyearsgipf hnaigtetkk tvrmdesten srrivtveqt
     4561 srvikfgdnq ksatdfdsfd sssaqkqkth ykvptpkkfv qgqfresdye sdidtrikpk
     4621 wappesdsee pryrkvqppt tkttrsssap vrqehvvspm efdtgptvlr kqtsmsqlrd
     4681 erkfdgqvkr qassrqeetl kpgsppefgy isrsdvkkaa nhvasrhmsd mtssfkskte
     4741 kfvtdiqsdl kqgkpilkqp vdgrasdade prtyreesrl sehgtkqidp dtgliyfkyd
     4801 fgyefgivlp gegkktvsst srsvqgqrra sdievpivhe fttrkengfa krsassssta
     4861 rqgkpagkfs gsktvkwept seseysesed irnarkrhse ggntmtapsl vipcspspsr
     4921 wdhttpspls lspslpslsp ryssatgpps nvdsagspwp ttngvasske viqpyleilp
     4981 kkaplfitpl rdiavvsgqt arfecivqae pqpnilwskd griienstsy eihyrngvcr
     5041 ltivrafped agtyactatn slgstvtsat llvpgvpspf ygtyrdqnls ersaesppky
     5101 valrdlflpq esdvktassn rqqqhnsstf eyawsvnrsy aggryreken ateecveits
     5161 rnpiarrley sdfqandsfr dvadfcfdre ystcnpsias prntvqgeiv mqttptvpaf
     5221 tgrpgdpspp vfeqifknar faqggnaife grvrgnpkpt vtwthkgapl leswkirmsy
     5281 ddktgavtlq inqigpgdeg eytcsaknqy geaicsvyiq pegfgpppqq qiggyrkeya
     5341 qtfqsteqks qtgsqsfqqr syqqtidkrs yvngtstsie dfkvdtfeyr llretefres
     5401 itrrfvgesd vqistvvdrs lgpvappqis qkprnsklie gsdavftaki sgnpkprltw
     5461 fkngqrires qrvemshsnq qatlrirval pedsghytll senpqgctvs saylaiessd
     5521 qvdqayqtpr esvktpqves tgesdsgkvl ppnfvrtctd rdategkmtr fdcrvtgrpy
     5581 pevtwyingq qvandlthki lvnesgnnsl mitnvsrada gvvtcvarnk agetsfqcnl
     5641 nviekeqvva pkfverfttt nvkegepvvf maravgtpvp ritwqkdgvp itpgpdvris
     5701 tdgsgastld ipcaklsdaa wyqctaqnva gstatrarlf vetpkgaape prrlnlprpt
     5761 kviepepapg peviylrhve rakpylpppe edrvyppprf iiplrdvhqi eggrihfear
     5821 iepvgdptmr vewyvngral dassratsif rfgfisldli siilqdsgey vcrvvsstgv
     5881 aesratlsvt pratieqasq hpdslqyiqq ledyskyqrq esveeissqr pafirplqdl
     5941 gelqegrnah feaqltpvsd ptmkvewykd grpitassri ttifnfgyvs lnilhlraed
     6001 agsytvravn rlgeavssas lrvyartsvt tdlgipeqqr yieaaeelea yqqamhqkyv
     6061 qeqpeptspp efkspikdqn sireggfahf earlepvgds dlrvewlkdg rpveassrit
     6121 tffnfgyval tikyvtihdv giytcraynr ageahttaql sviskndviy dsqhptglqk
     6181 iqtledssry srqvqeetqv tqaprflgtl kgtnkivegq rahfearvep qsdltmtiew
     6241 yhngkaitaa nriqtyhdfg yvaidilqvr pedagtytvv arnalgearl satmvvetrs
     6301 svdtssmhrt syektqrlee skfvepqyhi eeiskskpif vqplsdpkpv segknihlec
     6361 rlepmgdptm rvewfqngrp vtvgsrfrty ydfgfvaldi vhstildsge ytvratnhlg
     6421 tahtsacvrv igksdivtet qheqsleqiq mledaaryrk tqqeevtvmq apqftrplhn
     6481 ietveltnvh lecrlqpvgd atmkvewfvn grpvktghrf rpsyefdyva ldilgvyped
     6541 sgvytcqarn qlgeavtscs vrvhakkdll lesqhpegle riqyledasr ykrhelvdev
     6601 vkvkprfvta pknqenlheg qhahfeckle pvtdsnlkve wykngrpvti ghrfrpihdf
     6661 gyvaldvidl iaedsgtytc ravnlvgsde vscslscrst aqvltdtkne qgleqihyle
     6721 drskyqrred veetttqapi fttslnnvei kegqrahfec rlipvsdatm kvewfhnnkp
     6781 vkagsrfiet nsfgfvaldi myaypedsgt ytcrakniig eaitsatanv hskksiytet
     6841 qneetlqrlh hledtsryqr kttteeivtq apvftmpikd lkvaenqaah fearvipvgd
     6901 sklkvewlrn gvpiaasnrv ttmhdfgyva lnmkyvnped sgtytcravn dlgeavtsat
     6961 lfvqskaalq fesqhesals klqaledtsr yqrheeeeiv vkekpsftvq lngptnlveg
     7021 qsahyecrie pypdpnmkve wfhngkplst ghryrttcdf gfasldvltv yaedsgtytc
     7081 katnklgsaq ssikldvksr asiiretqhe salkkiqyle ddsrykrvdq edliiaekpk
     7141 fgrplknieh lpegksahle atltpvndpt mvvewfrdgr pipqghkfkt tydfgyvald
     7201 ilyaypedsg tymckarnav geavttcvis vdskqglfld tldaqrlqki reletvevkq
     7261 evekevvhqk pvfltplnnl dhlkegehah lecrvepind pnlkiewfvn gvavktghrf
     7321 rkthdfgyva ldilytyped sgtymckatn lageavntct ikvgsrrsil ldtqhpdgle
     7381 kireleaqgh parleveepp vtpprfvtel rgttevyegq tahfecqiep lhdanlrief
     7441 fhngkplpsa srfhvtfdfg yvaldightv pedageysvr ainalgqcvs sielrviprd
     7501 niildsqrpe gldkirelea qqpwkrpdip epqtrqrpvf tqplqnidai pegqtahfec
     7561 rlipvgdptl kvewfrneip letssritkv hdfgyvsldi nhvreedegv ymcrasnplg
     7621 eavttasmki kskasiqldt qhpeaqrkia qleadkpsrp eepekvfdkp iftqlltgpt
     7681 elwegqmary ecrvvpvgda slrfewying velkmgsrfh vshdfgyvtl dilkviteds
     7741 gvytckaink ageavssisl kvkarsaida dsiqpdawqk iqlkeaemnk vpemfvdttp
     7801 qqapvftkhl esydklvegq hvyleaqvep radpnlrvew fkngislqtg trlrstfdfg
     7861 lvtlsinglr pddsaiytck atnllgeavs tcslkivdrh wllgdtlhpd alpkidaleq
     7921 phvtsaeqpe piyeepvfit hlnnvecveg dnvhfecnve pskdptmkie wfingkplpt
     7981 garfkstydf gyvalditha yeedsgvviv katnskgsaq tsgtlkctsk qsiylqtqhp
     8041 qgeaglekvk eaedaylsky rrpdegpehe ypkpiwtvpl qpefklgefe plhlegqvep
     8101 kddpnlkiew yfngkvleha srfkmtsdfg fvtldltdvy erdsgiytck aynkageaft
     8161 sttiycstke niiersqhpk gkegleaiqd leeslkrqeg appeseeghp pvftsqfenl
     8221 tnlsegeiah feasltptgd qtmvvewfyn gkvleashri rtvyafgmvv levlgtkied
     8281 sgtyscratn kwgqaeisve lqcvdkskgq kpkftthiqs leglkdgqsa hfectlipvg
     8341 dpqmkvewfh ngqplrhssr fkmvsdfgfv vmdiagvmah dtgeyvckas nkygedftka
     8401 tlkcfgksgv yldslqpdsl arirelesfg geqpttpttp vaeppkfitq ivditrlveg
     8461 qsahfearlt pvndpdlkve wyyngkklph ghryrtfhdf givildilyc yeensgvyec
     8521 rafnkygedt tkatlkcfsk aslilesqlp kgmeggleki qtledsmirt kdekvveerg
     8581 kapmftvpls nidglreges ahfearlipt ddpklkvewf wngkplrtgs rfrtfcdfgf
     8641 vileispiyp edsgeyscra tneygeavtt ctmkctgkrs iilesqlpkg mestidkiae
     8701 leglgnepgq vtpeddtgkp pefvttpsdl tltenslahf ecrltpinds smrvewfhng
     8761 kpllagsrik tihdfgfvil evancyqrds glytckatnr hgeatvnckl qvrgrqgiil
     8821 epqlptnfkt gtesiqklee alykrdeilt eeetpnppkf tvelkdieve egapshfdcr
     8881 vepvgdstmr idwfhngrsf atgsrvhqin dfgfisldms ytyardsgey vcratnkwgs
     8941 attkatitck skktidfdsq lpsgmsgekl kelergpvse ppveeaprqp prfithiqsa
     9001 tvdesepvrf ecrvepkddp nlriewyrng kmipaghryr tmydmgfvsm dilyvypeds
     9061 geyvckaind lgedttrasl sckklpsiil qnqvpkgmkk sealmqmeat ikkytsevhl
     9121 teddlydadk kqpprfvtqi ldqtelvemn sskfecqlap vgdpnmkvew ffngkplphk
     9181 nrftpiydfg yvamnfgwvy pedsgeylcr atnlygmdet ravirtagkp giiyesqlpk
     9241 gmksiekire meaawqivpe eegeeekvra pptfvskpep vtveegdcsr fccrvtghpr
     9301 prvmwiingh tvvngsrykl tydgmyhldi pktrqydhgk veviarssvg esrtettltv
     9361 kprsddyrgv lknsprpwyd ygltqyqter qntelekvfd erhhnvsqgi eiatehlgqk
     9421 vfkepetdwq ksvkskknee yynklmtlee eqvlketrlr esshqfaipg ekvvadsvak
     9481 gmaqkyeetl eekkdektqe tvqppprplk kphttevdid vermkvtgky ppgpsestvh
     9541 grevhvakqk qtqkevkgdl eitrkitate ttevehkakt qervvqgptk pakppvftkk
     9601 iqpcrafeqe qarfevefdg dplptvkwyr edfpiqnsad lqiytfstks vlivrqvfme
     9661 dsgvfsviae nrggkakcsa nlvveerrrq pgrggvipps flstiqntsv ttgqlarfda
     9721 kvtgtkpldv ywlkngkkit adiryktlee dntytlllle tvpedsgkye cvainkagea
     9781 rcdaectvrg pqspaksakp ttpgvekapa vleplkdqti regtsvafsc risgkpvptv
     9841 qwkkgdkvik pskyfqmske gdfctlrise afpedegvyk ciaknpagdv ttsanlrvla
     9901 pdaadvlpkl tplkdqivse gqpaqfktqv tpskpkptiq wyregalipq spdfqmiheg
     9961 nnavlliatt yeedtgtftc rattsagtve tsaklivkkr tgayyevpae tgpgartgdk
    10021 pfvslgtgkd vilepipldk geleiplkpg detlpwrkgr vhhrprladt tpwrktkpks
    10081 rdtsldklqa tesqikpwte etvtlkktpq qareipkekl eevelkpaki ekkeirrpsl
    10141 etvdlkpftk ettketkiee kltrreeidk eiyeeeedsr flktsrkvde tvqrkkdevk
    10201 pwteekvtlk kskpekkelp ketletvelk pskigkgevr kpslekvdlk pvpttvtekv
    10261 sekvtektey leqedtslld vsrteevkkk rkekveekpe kpeqakpwte ekitlkkskp
    10321 vvkeieketl etvelkpski gkaelrkpsl ekvdlkpvpt tvtekvteki tekteyleqe
    10381 dtslldvsrt eevkkmrkek veekpeqakp wteekitlkk akhlpkeiek etvetvelkp
    10441 skitkseirk ssvekvdlkt vtketkeitq ktdyreeedt tllevtkdtq vkktrresip
    10501 vekvegpkpw teekvtlkka kpekkevpke kpetvelkps rpqkadiekp sledvdlkpi
    10561 pkkvtkekte kdkyieeedt sllevtkdte tttkvekkvk rreevekkpe tkpwtaekit
    10621 lkkakveqke irresieevt lkptktekrq vtketlekvd lqkvtdktqf veeedttlmk
    10681 vdkvekveee rvdkmatwrr drkpkekapy heeedksild vkrdeestek rkevpvpwar
    10741 gkkkptekap yheeedtsll dvkkdeesie kqkevpvpwa rgkkkpkeek pelvpqqvqp
    10801 eekpeeeetl ektqvpwlrd kkvarkiper keepvediks vtlkpvrrer apeekekpee
    10861 vtlkpvkrip reeeekeevq lkpipkrkpe ekpqeeiilk pfqkpkpeve eqekpklkpf
    10921 ekakpevpeq ekvqlkpfkr ekpevpekek velkpfkrek peetlvpqkv pekpeeeqps
    10981 eveevswrrk pkkkeaiipe kpvekveevk vvedkvikkq eetiedfksf tlkptkrgkk
    11041 leekeekeev tlkpvkrlpk eeetkaeikl kpvekkveek eakplkpema ptvpeeekpt
    11101 evsevpwkrg kkkpvekrve evtteekkve eeekevsels wrkpqkekvk evkkeqeved
    11161 ftavmlkptr kekkpeekee keeitlkpiq kvqkeeekpe avtlkpvkve kpeavekeev
    11221 peektelpwr rgkkpakkiv pqeevkpeei gkpeervpev keevpkkpkv ekpeeeklev
    11281 klkpvkrlek peeakpeevt lkpvkrlpke eeekeevklk pikrekpeee kpevklkpfk
    11341 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpidrkk peeekpevkl kpikrlekpe
    11401 eekpeevalk pvkrlpkeee ekeeiklkpi krekpeeekp evklkpfkrl ekpeeekpee
    11461 itlkpvkrrp keeeekeevk lkpikkekpe eekpeiklkp ikrlekpeee kpeevtlkpv
    11521 krlpkeeeek eevklkpikk ekpeeekpev klkpfkrlek peeekpeeat lkpvkrlpke
    11581 eeekeevklk pikkekpeee kpevklkpvr rlekpeeekp eevtlkpvkr lpkeeedkee
    11641 vklkpikkek peeekpevkl kpvkrlpkee eekeevklkp ikkekpeeek pevklkpvkr
    11701 lekpeeekpe evtlkpvkrl pkeeeekeev klkpirkekp eeekpeeikl kpvkrldkpe
    11761 eakpeevtlk pvkrlpkeee ereevklkpv kkekpeeekp eeiklkpvkr lekpeetkpe
    11821 evslkpvpkv vpekdeekpe eeapetsilp wrrgkktkkv vdfheevtvv pidqkkivse
    11881 vhekettive qkveakevie kkvvveevkp evveevtevs evswrkprkp kkpveekevv
    11941 elkpvekhee ivekveekit lkkvkkevpe veekkeiqlk pvpkkpkeee vpekitlkpv
    12001 kkekpeekpk eeeipelkpv klekpekvee teekeevmve itrkkpkkkp eevpvekeev
    12061 veteiteite ekpeeisvlp wrrpkkvtek kieveeikpe kvedkeveev tettevswrk
    12121 qrkpkkpiee eekpekvtlk pvkrekpeek pkeeipelkp iklekpekve eteekeevkv
    12181 evtrrrpkkk peeiqreeie vetkevvddv evekiekqei qiedrkrkrk qrtqvdisii
    12241 dkdkkiaprf iqklqpviae pestakftct vfgspvpeis wyrneqelha sekyimtvye
    12301 ttasleitkv keedagmysc rasnpagvat stvnlvifek eeegiaphfs tpikplmvee
    12361 hkpallecvv tgspvpevkw yrgeqelkpk kgreitfnpe tgeaklhile pteedetiyr
    12421 vravnkfgra ecranllinn vvkvskpevl rapkitrplp alvaergkpl tlsadfeskp
    12481 tpevkwfrng velapseknv iniyentael fipevtkkdg gkyeirvqnp vgearssgsv
    12541 tvkdrpdktd evkaprfiep lqpqivaege vvimetkves yptasfqwfh etrplestpq
    12601 vrivtqenks ilmikeikse yagtytcrae nvggsvtcta tinlletpwe etvelvsptf
    12661 vkrlspvrvm dgesanltcv vqgkptpkve wyhdskpike gkeitivqdt egvcslaite
    12721 vfpedageyt cravnpvgea vcttslivea yeyvpdseia ssivatsltt gqsgseedll
    12781 spketplfdt dteesapeii kklpqliptk dgeltrlevk vkgkpkpegk wykqgveivs
    12841 sqefqieefe dgtsvltiae typddtgeiv feahnplgvs ttmtylsveg ivgtkeyrkp
    12901 ewvthmeemq ealratqtvp rfiqeitdvy vkegdtavfe csysgnptpd vvwykndkml
    12961 mstqnlkvri ydddkktvlt ikhateeddg ayvckitset glatskaklh vtdvtdekkf
    13021 yeeeeeveeq tievkpkekp qkkklrkake vkekvkterv kvdkkevrke klvpkeqpee
    13081 kkivdieetq ilestdiiee kptevsqakk ivpiqepvlt eataslkkid dekpkdmvpk
    13141 peerakrvie eregivvvse vttedmapdf tveskvdraq itseslqtvt vsevhtetsv
    13201 qevrraeakq rkakktvvte eeifeerpke vvveekpkkk kktekvkvre evtieeiver
    13261 qreniareve eimemlhake fgpgeaplre latigylvrq gvsvneines lyrtenfpal
    13321 ktpeaqnalv qlvereghgp litqvlteet ttdesvvaat vgfrafmrmi elqhvtveev
    13381 lthfapeafr prawevteat eveteervte rvdvvrktev hfmerrderk avrtqdirdi
    13441 keyedtrqth ttlrarkdrk preetkedrd egvqvveiee iedteqekmk kkrkdveeie
    13501 eeietvivet dskgrlaqkk kkrddleeke dveeeeeiie kvrkelkpkr pkigrdevel
    13561 eeieefdvte dksmpsimvn vpsqrhqvvp ldrtteqapg kpelekaklt vdtitalteh
    13621 ivpvqekeis disrikpier kaslsispve pysttettvq astgefadtf kptsyeatpg
    13681 iipsegvqvs etlvsdagvt slsvskqeig rtaevsvtlq eattvyetmv rekevptedf
    13741 vtpqtvkaed tilpqlslsv yevheglted kleplktvat kprvnvtave plvveevrae
    13801 dkpgkyypel ivptevatqs visqrqrvte emhapekege yipgrlppsq vaqigisygn
    13861 etavvqqnvv qesegvfise kktetfeatt nvtvlegvsv stvqtqdtet sltieetkka
    13921 vadlnviemt pavtvetvps ekekeyqpgd kpdtktaets issleigsiq stvvqesegv
    13981 yrpdekptka laetsirpee hvmiseiqta dypsdfreel kyvqesgtvs vqlteakmiq
    14041 etlthdreer meemvkpeer mvdtsydvnr svevfqttsv ekeadlrvfe mpeshrgktv
    14101 pthpvvslev emtqpednlg evkkevpssg takvehvslq etivgetvva enlapvekdk
    14161 apetkvaeia mneveslhtt mvvvsekete ytqvsdvkga yastefttqv apmleevrsh
    14221 spteeylpdq kptsgiaqps hvplesitva vqelaekedl yktdvrpdqk tanvaltelt
    14281 sisipevmqv itassaetlk ketkmkeekg rveveelsst iisevisnet edvfttkatp
    14341 kdqkadfnis greaaettqv tamteaedla vkrpeeqkgk pqvdeltplt vshvetgeae
    14401 kelptaevpk ektaqpnifg rdvaetmqvl tvtsaeelaa pkapegqkgk pqveeltpls
    14461 vsqvvsqeae salpapeapt erkaqpdmtg rdiaetmevl tmtsadilae dvkpteqkgm
    14521 pgleefvpls vsqtvsteke dvlpspeapt tktaqpsltg rdiaetmqvv mmtsekelit
    14581 alapethkgi pnveeltplt vsqtvsheae dvfektaaps evtakpafsg revaqtsemm
    14641 ivtnvgelek qvlpdeqkgq aqldelmplt vsqvisgeve eglpeaekpk ektaqpgilg
    14701 rdiaettqvl tvtsaeelaa pkapegqkgk pqveelaplt vsqvvseeae gilpapeapt
    14761 erkaqpdmtg rdiaqtmevl tmtsvdtfte dkkpdeqkgh peleefvpls vtqtvsneme
    14821 dvlpspeaps tkiaqpsltg rdvaetmqil tmtseeelit aqtpeqhkgk pnveeltplt
    14881 vsqtvsqeae eilekaaapt evtakpafsg revaqtseim ivtnvgelek qvlpdeqkgq
    14941 pqldeltplt isqiisgeve eglpeaekpk ektaqpgilg rdiaettqvl tvtsaeelea
    15001 pkapegqkgk pqveelaplt vsqvvsqeae gilpvpeapt erkaqadltg rdvaemmevl
    15061 tmasvdtlte dkkpaeqkgv pgleefvpls vsqtvsteke evlpspeapt tkvaeasltg
    15121 rdiaetmqvv tmtseeelis arvpeqhkgi fnveeltslt vsqtishete eilekaaalg
    15181 evtakpaftg revaqtsetl ivtnvgelek qilpeeqkgk prvdemtsit vsqvvsgeve
    15241 erlpeaekpk ektaqpsmlg rdiaettqvl tmtsaeelaa pkapeeqkgk pqveelaplt
    15301 vsqvvsqeae gvlpvpeapt erqaqpdltg rdvaqitvvm smasadvmid dkkpeeqkgv
    15361 pgveefvpls vsqtvsteke dvlpspeapt tkmaqpsltg rdiaetmqvv tmtseeelit
    15421 alapethkgk pnveeltslt vsqtisqete vileksaaps eatakpaftg reiaqtseil
    15481 avlnvgelek hvlpeeqkgk pkleeltplt vsqiisgeae eglptaevpk ektaepsmlg
    15541 rdiaettqvl tmasaeelma pkapeeqkgk pqveelaplt isqvvsqeae gvlpvpeapt
    15601 erkaqpdltg rdvaqttvvm smanvdimie ekkpeeqrgi pgleefvpls vsqtvsteke
    15661 dvlpspevpt tkmaqpsltg hdvaetlqvl tmvseeqltt kapeeqkgkp nveeltsltv
    15721 sqtvsheaed ilekavapse atakpaflgr evaetsqvlt vttvgelqkd vlpeeqkgkp
    15781 qleelmpltv sqivsgeaee rlpeaekpke ktaepsmlgr diaettqvlt masaeellap
    15841 kvpeeqkgkp qveelapltv sqvvsqeaeg tlpipeapte rtaqpdltgr dvaetmevlt
    15901 masldtlied kkpaaqtgap gleefiplsv sqmvsteked vlpspevpte kmaqpsligr
    15961 dvaetmqvlt misamelttg hapdqqkgvp nveeltsltv sqtvshetee ilekavapst
    16021 vtakaalsgr evaetsqvlt vsnveefeks ilpegqkgkp rldeltsitv sqvlsnelee
    16081 tlpspekpse kiaqpqmigr evaeqtevlt vtnveelkkl ekpegqtgkp dveemsfitv
    16141 sevfstemek empspevpke rtalpqlssi evaettevlt misaeellks kapeeqkvqp
    16201 kldeltpltv shvvseeaee alptaeipke ktaqpsllgr dvaetsqvlt masaeellaa
    16261 kvpeeqkgkp qieelapltv sqvvsqeaee alpsaevpve reaqpsmtsr dvaqtmlvts
    16321 msnvdimaes kipeeqkgvp gleefvslsv sqtvsteked vlpspevpte kmaqpsligr
    16381 dvaettqilt misatelttd rapeqqkgvp nveeltsltv sqavshetee ilekleapgt
    16441 vtakaalsgr evaetsqvlt vsnveefeks vlpeeqkgkp rldeltsitv sqvvsnelee
    16501 tlpspekpse kvaqpqmigr evaektevlt vsnveemqkl ekpegqkgip dveemsfitv
    16561 sevvsteaek elpapeapke rkalpqlsgi evaeksevlt mtmteefakp kapeeqkgkp
    16621 qleellslsv sevalgevek glptpdkpte etakpsllgi qvaeksqvia ssitgelqes
    16681 ikpdtkqiip eqipfesieq sqtltqeres tfvvekttts ataevsfrvs eslevtqvta
    16741 tekeskevik gvakeasaqa dvierkvalk tevqpedmas efkvkkpegk takgvdeiqq
    16801 gvivtehlta hefeselpes vlpfaklarv siqadhlehi vetteapaqq hhititqhtt
    16861 khdlpqqqsi esdtevieey ttkfrrgste dataavktkk tivrkkpktt geedivvvie
    16921 eelddikpki psipkkqfmp ieevttvsdv eeivsaride aeeivpkeee kvevtitevq
    16981 dtdkkkkvtk kqfvrrrgkt rqvteeitve eegkapettv vtkpveiiee dieerpkiek
    17041 eeyvveevpe ektiplkprk kltptvqqve eqvtvteeit kegkprkttk kkvitrkgke
    17101 qqvtevvtve eqgkapitsv tegpveevve eiiqklpapk yakpseveev reqvtvteev
    17161 trlgkpkktt kkkiikrkgk eqqvtevvtv eeegkapttt vtegpveevv ieeikelpip
    17221 efvkptevee vreqvtvtee ltkmgkpkkt ikkkiikrkg keqqvtevvt veeqgkapvt
    17281 tvkegpveev veetitplla peyakpseve evreqvtvtq evtklgkpkk ttkkkiikrk
    17341 gkeqqvteiv tveeegkapk ttvtegpvee iieeeikpef ikpreveevr eqvtvtqevt
    17401 regkpkktik kkiikkkgke qqvtevvtve eqgkapvttv tegpveevvd etvkalpape
    17461 yakpseveev reqvtvtqev tklgkpkktt kkkiikrkgk eqqvteivtv eeegkapktt
    17521 vtegpveeii eeeikpefik preveevreq vtvtqevtre gkpkktikkk iikkkgkeeq
    17581 vtevvtveeq gkapvttvne gpveevvdet vkalpapeya epseveevre qvtvtqevtk
    17641 lgkpkkttkk kiikrkgkeq qvteivtvee egkapkttvt egpveeiiee eikpefikpr
    17701 eveevreqvt vtqevtregk pkktikkkii kkkgkeeqvt evvtveeqgk apvttvnegp
    17761 veevvdetvk alpapeyaep seveevreqv tvtqevtklg kpkkttkkki ikrkgkeqqv
    17821 teivtveeeg kapkttvteg pveeiieeei kpefikprev eevreqvtvt qevtregkpk
    17881 ktikkkiikk kgkeqqvtel vtveeqgkap vttvtegpve evvdetvkal papeyakpse
    17941 veevreqvtv tqevtklgkp kkttkkkiik rkgkeqqvte ivtveeegka pkttvtegpv
    18001 eeiieeeikp efikprevee vreqvtvtqe vtregkpkkt ikkkiikkkg keqqvtevvt
    18061 veeqgkapvt tvtegpveev vdetvkalpa peyakpseve evreqvtvtq evtklgkpkk
    18121 ttkkkiikrk gkeqqvteiv tveeegkapk ttvmegpvee vvieeiqelp ipefvkptev
    18181 eevreqvtvt eevtrlgkpk kttkkkiikr kgkeqqvtei vtveeqgkap vttvtegpve
    18241 evvdetiksl papeyakpse veevreqvtv tqevtklgkp kkttkkkivk rkgkeqqvte
    18301 ivtveeegka pkttvtegpv eevveeevkp llapefvkps eveevheqvt vtqevtregk
    18361 pmktikkkii kkkgkaqqvt evvtveeegk vpvttvkegp veevvdetvk alpapkyakp
    18421 seveevreqv tvtqeitklg kpkkttkrkv ikrkgkeqqv tevvtveeeg kapettvteg
    18481 pveevleeei kplpgpefvk pseveevreq vtvteevtre gkpkktikkk iikkkgkeqq
    18541 vteivtveeq gkapvtsvte gpveevvdet ikqlpapkya kpseveevre qvtvtqemtk
    18601 lgkpkkttkk kiikrkgkeq qvteivtvee egkapkttmt egpveevvee eikplpalef
    18661 vkpseveevr eqvtvteevt regkpkktik kkiikkkgke qqitevvtve eqgkapvttv
    18721 tkapveeivd etikelpale fakpseveev reqvtvtkev tkegkpkkti krkvikkkgk
    18781 eqqvteivtv eeegkapktt itegpveevv eeeikplpal efvkpsevee vreqvtvtee
    18841 vtregkpkkt ikkkiikkkg keqqvtevvt veeqgkapvt tvqegpveev idetikslpa
    18901 peyakpseve eireqvtvte eltklgkpkk ttkkkitkrk gpeqqiteiv tveeegkapv
    18961 ttvtegpvee vvkevlkplp vpkvvkpsev eeireqvtvt eemtkegkpk kiikkkvikr
    19021 kgreqtvqev vtveekgkap vttvtvgpte eiveetvktl papeyakpse veevreqvtv
    19081 tqevtkegkp kkttkkkvik rkgkeqqvte vvtveeqgka pvttvtegpv eevveellkp
    19141 liapeyvkps eveevreqvt vteeltkegk pkkvikkkvi krrgreqqvk evvtveeqgk
    19201 apvttvtegp teeiveetik alpapedakp seveevreqv tvtqevtkeg kpkktikkkv
    19261 tkrkgkeqqv tevvtveekg kapvttvteg pveeiveetv kalpapelvk ptevtevpee
    19321 ltvtevvtke gktkkivkkk vpkpkkegie apeeapeevp veetkkiikl qrlkvskfes
    19381 tkldiqetsl gkpqfaqikl rktpvakrps kkdekfpril lrsriiaiew ppspknlkvd
    19441 tfepielqng ilsrnvdeaa alpkpkkkki kriekeiakl ekldqefeel kkelplekle
    19501 epileekper kvkkkkkped kakegvkpql mrisikeqkp ikpkiaepat plfaqiklkk
    19561 apakpkkkpe eevskfpkil lrsritahew ppsvkypais vlepnyiqng ilsrtmeeal
    19621 klkkvkhkkm klpekeltel ekldqefeel kkvplekvee itpyerkpkp ekpeeekpkk
    19681 lvigkgkrrp aeeedletvq lkkipekips apeeaekvpk kkdevdkpkk ekeekeypel
    19741 kpfepydiea peveleerek peypepekpk ekkvpgkkpk kerktpaqet eavpivpgvp
    19801 kpkepeeeee ikrriperpp pteepekikl kpwkkgekpe eeekkpedyi kfvpkdddiv
    19861 etvevtttve etpdkkqrki kkkktitkpk dekpkiieet tieeegfepv vtveefdvdk
    19921 yeavpvesve kaapveeike eivtseiite egtkktvrkk rvtkkregke rvteevtvee
    19981 egkepitati elhedtisei heqakhkrpt kpkadeklae dvtektveqq eiieevkevk
    20041 ptkkvikkek pvkakdevte evveeiiiee pekqeakpiv eeeetviite hdvrkapkkk
    20101 kevvikeeee ifetietpth kiirkkirpm kkgekevvve eiiqkpreet vemeeqqive
    20161 vvetptkkiv kkkkevvkdd gvpeelveei iiekpqkkka kptveeekkk vkvtkvdtkk
    20221 apekpeevve eeeeiietie tptkkiirkk pkdkkdkiie eviqktieea kameeqeive
    20281 vietptkkvi kkktttieeg kapeekveei viekpkevie eeeeiietie tptkrivrkk
    20341 pkdkkdkive eviqktieea ktmkeqeiie vietptktvi kkkkatikeg kapeeeveei
    20401 viekpikeka kkkilpkegv eitevitets veelieeelk peeakpseev eeekvkitev
    20461 aikkvpkkkk pskaveaeek eevveeikpe kpkeekakpt vtekegievt evisevapek
    20521 lpeeakpkkk kateiketee kvitkkvkvk eapkeavpge eeqpaeevde lvpekpkktk
    20581 akkevtpqds ivttevvpit keeelevekp eeekatvvee keekakveek kvtvkkapkk
    20641 klkpteaeee qveevveeit peepkkrkak kavipkeeiq ttevipgita eeipeeekpe
    20701 eekavpkeee kveeeakvte vsvkkkerkk kekpvtngev ieekpkvkei peekpkeevp
    20761 qvpeekekpe eekpkevkpk ekkpkeekpk kpieekpeev kpeeekpevk peeekpkeek
    20821 pkkkkpaekk pkiseeerpe dkveeipekk peevktreei ipkdtvqtte vipetvaeei
    20881 ptkkpkerka vpveekeekv eitevpveka pekkeekvpe ekpteaapek pkeakvmkkk
    20941 kkkpakkdei eewvepeyer pvlepmpeki vwepkkkkek kplpeslqkl vpqkierkem
    21001 kptklkysep aetvqfasik lkkvavpkkk eadetkfpki mlrsrmtfvg dyppelqipk
    21061 ityleenpvq tgilsrntee alkvlkrkvk kvklsekevt qlekldlefe elkkvpleki
    21121 edksvyerqp kkpaekpeep kkvpigkgkv peeekvepek vklkkvpekk peeveeplkk
    21181 pkkpepeeev kpekkkkpkv klehdelkpl dfdrpeleky epeeyekpee papepvpkpy
    21241 ekepkkkpdq eveqiplkkg kpkspepdee pevkfriptk ekpeeepeqi tlkpwkkekp
    21301 eeekekeipk keeekvpkpp keepedvtvq rkaktkkpke kpkeekeeev tvkkpeeekp
    21361 eevaeevtlk kepekpveea paevtikkpv veekeveeee titevilkkk pekpeevvee
    21421 avvkkpkkkk pvkeeevvee vtvtkvvtee ekeeekvvik kpkkksipei teevteeitl
    21481 kkpeekapee vteeikkpkk kkpvkeevae eitikkpvve ekkeeevpee vtlkpkkpee
    21541 kpvieevtee itlkkpvpee vteevaikkp kkkkpveeea adeitlkkpv vvekeeeeit
    21601 eevtlqkkkk pekkpvpeei seeitlkkpv pkeeekapee vteeiaikkp kkkkpvkeea
    21661 adevtikkpv veekkeeetp eevtlkprkp kekpvpeevk eevtlkkpvp eekekvpeev
    21721 teeiaikkpk kkkpveeeaa devtikkpvv vekeeeeite eitlgkkkkp ekkpvpeeit
    21781 eeitlkkpvp keeekapeev tekievkkpk kkkpveeeaa devtikkpvv vekeeeeapa
    21841 eitlkrrpke kpveeiteev teeitikkpt peekekapee vsekvevkkp kkkkpvveea
    21901 adevtikkvv veekkeeeap eevtlkprpk kksvteeeva evtltkpkpi eeekkpeese
    21961 ltikkkpkkk pvieeeaaev tikkpvpvep deapeeitir kkkkpekkpk vteeveetav
    22021 tikkarapek pteeeeittd vqikkkkpqk kveevaadel tikkieeveq peeeeevqef
    22081 tfkrkppklp pkpieeiyed vtlrklrpkr ksrpdikeit etenvtfrpr ttktkedveq
    22141 efkislntye eedismsgkv rlkpkkkplt yseetgeeti riiqevedds gpiieeiies
    22201 deegkdeysi eeldtdemrl plrrkkkkvp kpykvediee gdvklklkre rkysieeade
    22261 slalklkskr rvstydeeea tlsitreedi seeveieyvv rdgdtmfsic syvaetdeai
    22321 nlvegervyv idhtnqdwwf vkkhlteekg wvpaqyllne vhytlylqrk lhekidklpv
    22381 fekpapgdks sapkfveklq pihtpdgyti qfecqvegvp rpqitwfrqt aiikpspdfq
    22441 myydddnvat liirevfped agtftcvakn aagfasstte liveaplsdh gsdltgpsrk
    22501 slsresslad ilegipptfs rkpkakyvde gedvilecrl vavpepeitw yykdtqittk
    22561 eniiiatesd mhmycsviki tkvqkkqegk ytivarnreg eatieipmkv ktgkseppei
    22621 leplqsyvvr egetvvlstq ivgnpapkvt wfkngkplkg ltpkqdghvn tltliqpqvs
    22681 dtgeysvvat ndlgtaetka tltveinlkp slainfqqht fehfsvtkei psgapepplf
    22741 terfqeltvp ekgtfklvak vtgnpvpevt wlrnnkplek spnitetydg enivleirna
    22801 nsevdtgdyk cvasnpvgka shgarvtvdv dkvtftktle kevvvdeykt leltcetsht
    22861 vstkwwhndk eisgmdhrei ieegrvhklv ikktnptdeg tykctvknqs tssnvtvkat
    22921 kpefvkklqd cevkerdvai leveitsqtv dvkwykdgeq lgpgkeklef vkegtvrkll
    22981 irstsvhdeg eytcslldqe ctaevtviel ppeiitkmqd vtiargerat fdieltkgda
    23041 lvrwfkdgqe lqfsehvrls idgkrqklki ydtesedagv yscevgqqks sakltveepa
    23101 vefitklpdv tlvplnsdav fvielsqdvp vtwmrktqvi kespkytiid egkvkklivk
    23161 kcttedisey tamvanvkts srlrveviet ppkispdtpr kykvrkdedv divvkfsgtp
    23221 kpedewtvng rvvtkskrai ptideesavl tirkiqeedv gdytlklvns vgetsieinv
    23281 vivqvpsapg apeplettnd svtlhwkkpd sdgnspivey ilehqektet twtkitetit
    23341 etthkvtklt tnkeytfrvt avneagpget sptspyvkis kpstaeppvi leplksivig
    23401 lgetvtlscv iggtpapeit wlrndeifed tsityenhit kytirettet ssakftvkak
    23461 nnvgsaettc elkiqespki iydenltnqk lpmngqwkie iqitgfpkpe vtwtkngkev
    23521 dekrvsihre ertstvsiss lvredtatym akaenqagss svdlhlkvid kpskpqgpvv
    23581 fkeirqdrvt lewrppaddg gielekytie kcepgktwvk vvdidkeves fcvhklqqna
    23641 eykfriiarn avgaseples dtvkmrtsfe ppgaprgple vsgmtktsft ikwqppendg
    23701 gtpitdyiie ikeeskktwq kigstkhett hfsvsdlktd vpynfritak nsvgsgppyv
    23761 aeepiapgrr ikitkltess myallgifpp tkivisktpp sspqhvhvtn itsksatlsw
    23821 sppastggte ltgyviekrp ligkgarwtk vvtldattlq ycvenlkese flfrifaenn
    23881 qglsvpanse pvtlkthasv pspptaplei rqiaantvvi swgrpdsdgg aplegykvai
    23941 rdtkrtmwme varvnteiqk lnirdlqenh mylirifarn eiglsdples eepfkiipas
    24001 eltvvepiae atekgetasi sfstentssw lrehnmdadi hsyararllr qdeyffriwh
    24061 yakklfe
//
LOCUS       XP_034182810           24050 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034182810
VERSION     XP_034182810.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182810.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24050
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..24050
                     /product="sallimus isoform X4"
                     /calculated_mol_wt=2717178
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16482..>16634
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22288..22340
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22293,22295,22301..22302,22319..22321,22334,22336,
                     22338..22339)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22376..22466
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22393..22397
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22406..22410
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22432..22436
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22446..22451
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22459..22462
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22500..22593
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22517..22521
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22530..22534
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22555..22563
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22573..22578
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22586..22589
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22601..22687
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22618..22622
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22631..22635
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22653..22657
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22667..22672
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22680..22683
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22721..22811
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22738..22742
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22751..22755
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22776..22780
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22791..22796
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22804..22807
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22824..22901
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22834..22838
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22845..22849
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22870..22874
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22884..22889
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22905..22990
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22922..22926
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22934..22938
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22960..22964
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22974..22979
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22983..22986
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22995..23079
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23012..23016
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23024..23028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23049..23053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23063..23068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23072..23075
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23185..23265
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23193..23197
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23206..23210
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23231..23235
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23245..23250
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23258..23261
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23269..23360
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23269,23334,23349)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23350..23351,23353..23354)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23371..23457
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23388..23392
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23401..23405
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23423..23427
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23437..23442
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23450..23453
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23481..23551
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23481..23484
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23493..23497
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23517..23521
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23531..23536
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23544..23547
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23555..23647
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23555,23621,23636)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23625..23940
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23637..23638,23640..23641)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..24050
                     /gene="sls"
                     /coded_by="XM_034326919.2:755..72907"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmeyedtr qthttlrark
    13441 drkpreetke drdegvqvve ieeiedteqe kmkkkrkdve eieeeietvi vetdskgrla
    13501 qkkkkrddle ekedveeeee iiekvrkelk pkrpkigrde veleeieefd vtedksmpsi
    13561 mvnvpsqrhq vvpldrtteq apgkpeleka kltvdtital tehivpvqek eisdisrikp
    13621 ierkaslsis pvepysttet tvqastgefa dtfkptsyea tpgiipsegv qvsetlvsda
    13681 gvtslsvskq eigrtaevsv tlqeattvye tmvrekevpt edfvtpqtvk aedtilpqls
    13741 lsvyevhegl tedkleplkt vatkprvnvt aveplvveev raedkpgkyy pelivpteva
    13801 tqsvisqrqr vteemhapek egeyipgrlp psqvaqigis ygnetavvqq nvvqesegvf
    13861 isekktetfe attnvtvleg vsvstvqtqd tetsltieet kkavadlnvi emtpavtvet
    13921 vpsekekeyq pgdkpdtkta etsissleig siqstvvqes egvyrpdekp tkalaetsir
    13981 peehvmisei qtadypsdfr eelkyvqesg tvsvqlteak miqetlthdr eermeemvkp
    14041 eermvdtsyd vnrsvevfqt tsvekeadlr vfempeshrg ktvpthpvvs levemtqped
    14101 nlgevkkevp ssgtakvehv slqetivget vvaenlapve kdkapetkva eiamnevesl
    14161 httmvvvsek eteytqvsdv kgayasteft tqvapmleev rshspteeyl pdqkptsgia
    14221 qpshvplesi tvavqelaek edlyktdvrp dqktanvalt eltsisipev mqvitassae
    14281 tlkketkmke ekgrveveel sstiisevis netedvfttk atpkdqkadf nisgreaaet
    14341 tqvtamteae dlavkrpeeq kgkpqvdelt pltvshvetg eaekelptae vpkektaqpn
    14401 ifgrdvaetm qvltvtsaee laapkapegq kgkpqveelt plsvsqvvsq eaesalpape
    14461 apterkaqpd mtgrdiaetm evltmtsadi laedvkpteq kgmpgleefv plsvsqtvst
    14521 ekedvlpspe apttktaqps ltgrdiaetm qvvmmtseke litalapeth kgipnveelt
    14581 pltvsqtvsh eaedvfekta apsevtakpa fsgrevaqts emmivtnvge lekqvlpdeq
    14641 kgqaqldelm pltvsqvisg eveeglpeae kpkektaqpg ilgrdiaett qvltvtsaee
    14701 laapkapegq kgkpqveela pltvsqvvse eaegilpape apterkaqpd mtgrdiaqtm
    14761 evltmtsvdt ftedkkpdeq kghpeleefv plsvtqtvsn emedvlpspe apstkiaqps
    14821 ltgrdvaetm qiltmtseee litaqtpeqh kgkpnveelt pltvsqtvsq eaeeilekaa
    14881 aptevtakpa fsgrevaqts eimivtnvge lekqvlpdeq kgqpqldelt pltisqiisg
    14941 eveeglpeae kpkektaqpg ilgrdiaett qvltvtsaee leapkapegq kgkpqveela
    15001 pltvsqvvsq eaegilpvpe apterkaqad ltgrdvaemm evltmasvdt ltedkkpaeq
    15061 kgvpgleefv plsvsqtvst ekeevlpspe apttkvaeas ltgrdiaetm qvvtmtseee
    15121 lisarvpeqh kgifnveelt sltvsqtish eteeilekaa algevtakpa ftgrevaqts
    15181 etlivtnvge lekqilpeeq kgkprvdemt sitvsqvvsg eveerlpeae kpkektaqps
    15241 mlgrdiaett qvltmtsaee laapkapeeq kgkpqveela pltvsqvvsq eaegvlpvpe
    15301 apterqaqpd ltgrdvaqit vvmsmasadv middkkpeeq kgvpgveefv plsvsqtvst
    15361 ekedvlpspe apttkmaqps ltgrdiaetm qvvtmtseee litalapeth kgkpnveelt
    15421 sltvsqtisq etevileksa apseatakpa ftgreiaqts eilavlnvge lekhvlpeeq
    15481 kgkpkleelt pltvsqiisg eaeeglptae vpkektaeps mlgrdiaett qvltmasaee
    15541 lmapkapeeq kgkpqveela pltisqvvsq eaegvlpvpe apterkaqpd ltgrdvaqtt
    15601 vvmsmanvdi mieekkpeeq rgipgleefv plsvsqtvst ekedvlpspe vpttkmaqps
    15661 ltghdvaetl qvltmvseeq lttkapeeqk gkpnveelts ltvsqtvshe aedilekava
    15721 pseatakpaf lgrevaetsq vltvttvgel qkdvlpeeqk gkpqleelmp ltvsqivsge
    15781 aeerlpeaek pkektaepsm lgrdiaettq vltmasaeel lapkvpeeqk gkpqveelap
    15841 ltvsqvvsqe aegtlpipea ptertaqpdl tgrdvaetme vltmasldtl iedkkpaaqt
    15901 gapgleefip lsvsqmvste kedvlpspev ptekmaqpsl igrdvaetmq vltmisamel
    15961 ttghapdqqk gvpnveelts ltvsqtvshe teeilekava pstvtakaal sgrevaetsq
    16021 vltvsnveef eksilpegqk gkprldelts itvsqvlsne leetlpspek psekiaqpqm
    16081 igrevaeqte vltvtnveel kklekpegqt gkpdveemsf itvsevfste mekempspev
    16141 pkertalpql ssievaette vltmisaeel lkskapeeqk vqpkldeltp ltvshvvsee
    16201 aeealptaei pkektaqpsl lgrdvaetsq vltmasaeel laakvpeeqk gkpqieelap
    16261 ltvsqvvsqe aeealpsaev pvereaqpsm tsrdvaqtml vtsmsnvdim aeskipeeqk
    16321 gvpgleefvs lsvsqtvste kedvlpspev ptekmaqpsl igrdvaettq iltmisatel
    16381 ttdrapeqqk gvpnveelts ltvsqavshe teeileklea pgtvtakaal sgrevaetsq
    16441 vltvsnveef eksvlpeeqk gkprldelts itvsqvvsne leetlpspek psekvaqpqm
    16501 igrevaekte vltvsnveem qklekpegqk gipdveemsf itvsevvste aekelpapea
    16561 pkerkalpql sgievaekse vltmtmteef akpkapeeqk gkpqleells lsvsevalge
    16621 vekglptpdk pteetakpsl lgiqvaeksq viassitgel qesikpdtkq iipeqipfes
    16681 ieqsqtltqe restfvvekt ttsataevsf rvseslevtq vtatekeske vikgvakeas
    16741 aqadvierkv alktevqped masefkvkkp egktakgvde iqqgvivteh ltahefesel
    16801 pesvlpfakl arvsiqadhl ehivetteap aqqhhititq httkhdlpqq qsiesdtevi
    16861 eeyttkfrrg stedataavk tkktivrkkp kttgeedivv vieeelddik pkipsipkkq
    16921 fmpieevttv sdveeivsar ideaeeivpk eeekvevtit evqdtdkkkk vtkkqfvrrr
    16981 gktrqvteei tveeegkape ttvvtkpvei ieedieerpk iekeeyvvee vpeektiplk
    17041 prkkltptvq qveeqvtvte eitkegkprk ttkkkvitrk gkeqqvtevv tveeqgkapi
    17101 tsvtegpvee vveeiiqklp apkyakpsev eevreqvtvt eevtrlgkpk kttkkkiikr
    17161 kgkeqqvtev vtveeegkap tttvtegpve evvieeikel pipefvkpte veevreqvtv
    17221 teeltkmgkp kktikkkiik rkgkeqqvte vvtveeqgka pvttvkegpv eevveetitp
    17281 llapeyakps eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk
    17341 apkttvtegp veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk
    17401 gkeqqvtevv tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt
    17461 qevtklgkpk kttkkkiikr kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe
    17521 fikpreveev reqvtvtqev tregkpkkti kkkiikkkgk eeqvtevvtv eeqgkapvtt
    17581 vnegpveevv detvkalpap eyaepsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg
    17641 keqqvteivt veeegkapkt tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr
    17701 egkpkktikk kiikkkgkee qvtevvtvee qgkapvttvn egpveevvde tvkalpapey
    17761 aepseveevr eqvtvtqevt klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv
    17821 tegpveeiie eeikpefikp reveevreqv tvtqevtreg kpkktikkki ikkkgkeqqv
    17881 telvtveeqg kapvttvteg pveevvdetv kalpapeyak pseveevreq vtvtqevtkl
    17941 gkpkkttkkk iikrkgkeqq vteivtveee gkapkttvte gpveeiieee ikpefikpre
    18001 veevreqvtv tqevtregkp kktikkkiik kkgkeqqvte vvtveeqgka pvttvtegpv
    18061 eevvdetvka lpapeyakps eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt
    18121 eivtveeegk apkttvmegp veevvieeiq elpipefvkp teveevreqv tvteevtrlg
    18181 kpkkttkkki ikrkgkeqqv teivtveeqg kapvttvteg pveevvdeti kslpapeyak
    18241 pseveevreq vtvtqevtkl gkpkkttkkk ivkrkgkeqq vteivtveee gkapkttvte
    18301 gpveevveee vkpllapefv kpseveevhe qvtvtqevtr egkpmktikk kiikkkgkaq
    18361 qvtevvtvee egkvpvttvk egpveevvde tvkalpapky akpseveevr eqvtvtqeit
    18421 klgkpkkttk rkvikrkgke qqvtevvtve eegkapettv tegpveevle eeikplpgpe
    18481 fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk eqqvteivtv eeqgkapvts
    18541 vtegpveevv detikqlpap kyakpsevee vreqvtvtqe mtklgkpkkt tkkkiikrkg
    18601 keqqvteivt veeegkapkt tmtegpveev veeeikplpa lefvkpseve evreqvtvte
    18661 evtregkpkk tikkkiikkk gkeqqitevv tveeqgkapv ttvtkapvee ivdetikelp
    18721 alefakpsev eevreqvtvt kevtkegkpk ktikrkvikk kgkeqqvtei vtveeegkap
    18781 kttitegpve evveeeikpl palefvkpse veevreqvtv teevtregkp kktikkkiik
    18841 kkgkeqqvte vvtveeqgka pvttvqegpv eevidetiks lpapeyakps eveeireqvt
    18901 vteeltklgk pkkttkkkit krkgpeqqit eivtveeegk apvttvtegp veevvkevlk
    18961 plpvpkvvkp seveeireqv tvteemtkeg kpkkiikkkv ikrkgreqtv qevvtveekg
    19021 kapvttvtvg pteeiveetv ktlpapeyak pseveevreq vtvtqevtke gkpkkttkkk
    19081 vikrkgkeqq vtevvtveeq gkapvttvte gpveevveel lkpliapeyv kpseveevre
    19141 qvtvteeltk egkpkkvikk kvikrrgreq qvkevvtvee qgkapvttvt egpteeivee
    19201 tikalpaped akpseveevr eqvtvtqevt kegkpkktik kkvtkrkgke qqvtevvtve
    19261 ekgkapvttv tegpveeive etvkalpape lvkptevtev peeltvtevv tkegktkkiv
    19321 kkkvpkpkke gieapeeape evpveetkki iklqrlkvsk festkldiqe tslgkpqfaq
    19381 iklrktpvak rpskkdekfp rillrsriia iewppspknl kvdtfepiel qngilsrnvd
    19441 eaaalpkpkk kkikriekei aklekldqef eelkkelple kleepileek perkvkkkkk
    19501 pedkakegvk pqlmrisike qkpikpkiae patplfaqik lkkapakpkk kpeeevskfp
    19561 killrsrita hewppsvkyp aisvlepnyi qngilsrtme ealklkkvkh kkmklpekel
    19621 telekldqef eelkkvplek veeitpyerk pkpekpeeek pkklvigkgk rrpaeeedle
    19681 tvqlkkipek ipsapeeaek vpkkkdevdk pkkekeekey pelkpfepyd ieapevelee
    19741 rekpeypepe kpkekkvpgk kpkkerktpa qeteavpivp gvpkpkepee eeeikrripe
    19801 rpppteepek iklkpwkkge kpeeeekkpe dyikfvpkdd divetvevtt tveetpdkkq
    19861 rkikkkktit kpkdekpkii eettieeegf epvvtveefd vdkyeavpve svekaapvee
    19921 ikeeivtsei iteegtkktv rkkrvtkkre gkervteevt veeegkepit atielhedti
    19981 seiheqakhk rptkpkadek laedvtektv eqqeiieevk evkptkkvik kekpvkakde
    20041 vteevveeii ieepekqeak piveeeetvi itehdvrkap kkkkevvike eeeifetiet
    20101 pthkiirkki rpmkkgekev vveeiiqkpr eetvemeeqq ivevvetptk kivkkkkevv
    20161 kddgvpeelv eeiiiekpqk kkakptveee kkkvkvtkvd tkkapekpee vveeeeeiie
    20221 tietptkkii rkkpkdkkdk iieeviqkti eeakameeqe ivevietptk kvikkkttti
    20281 eegkapeekv eeiviekpke vieeeeeiie tietptkriv rkkpkdkkdk iveeviqkti
    20341 eeaktmkeqe iievietptk tvikkkkati kegkapeeev eeiviekpik ekakkkilpk
    20401 egveitevit etsveeliee elkpeeakps eeveeekvki tevaikkvpk kkkpskavea
    20461 eekeevveei kpekpkeeka kptvtekegi evteviseva peklpeeakp kkkkateike
    20521 teekvitkkv kvkeapkeav pgeeeqpaee vdelvpekpk ktkakkevtp qdsivttevv
    20581 pitkeeelev ekpeeekatv veekeekakv eekkvtvkka pkkklkptea eeeqveevve
    20641 eitpeepkkr kakkavipke eiqttevipg itaeeipeee kpeeekavpk eeekveeeak
    20701 vtevsvkkke rkkkekpvtn gevieekpkv keipeekpke evpqvpeeke kpeeekpkev
    20761 kpkekkpkee kpkkpieekp eevkpeeekp evkpeeekpk eekpkkkkpa ekkpkiseee
    20821 rpedkveeip ekkpeevktr eeiipkdtvq ttevipetva eeiptkkpke rkavpveeke
    20881 ekveitevpv ekapekkeek vpeekpteaa pekpkeakvm kkkkkkpakk deieewvepe
    20941 yerpvlepmp ekivwepkkk kekkplpesl qklvpqkier kemkptklky sepaetvqfa
    21001 siklkkvavp kkkeadetkf pkimlrsrmt fvgdyppelq ipkityleen pvqtgilsrn
    21061 teealkvlkr kvkkvklsek evtqlekldl efeelkkvpl ekiedksvye rqpkkpaekp
    21121 eepkkvpigk gkvpeeekve pekvklkkvp ekkpeeveep lkkpkkpepe eevkpekkkk
    21181 pkvklehdel kpldfdrpel ekyepeeyek peepapepvp kpyekepkkk pdqeveqipl
    21241 kkgkpkspep deepevkfri ptkekpeeep eqitlkpwkk ekpeeekeke ipkkeeekvp
    21301 kppkeepedv tvqrkaktkk pkekpkeeke eevtvkkpee ekpeevaeev tlkkepekpv
    21361 eeapaevtik kpvveekeve eeetitevil kkkpekpeev veeavvkkpk kkkpvkeeev
    21421 veevtvtkvv teeekeeekv vikkpkkksi peiteevtee itlkkpeeka peevteeikk
    21481 pkkkkpvkee vaeeitikkp vveekkeeev peevtlkpkk peekpvieev teeitlkkpv
    21541 peevteevai kkpkkkkpve eeaadeitlk kpvvvekeee eiteevtlqk kkkpekkpvp
    21601 eeiseeitlk kpvpkeeeka peevteeiai kkpkkkkpvk eeaadevtik kpvveekkee
    21661 etpeevtlkp rkpkekpvpe evkeevtlkk pvpeekekvp eevteeiaik kpkkkkpvee
    21721 eaadevtikk pvvvekeeee iteeitlgkk kkpekkpvpe eiteeitlkk pvpkeeekap
    21781 eevtekievk kpkkkkpvee eaadevtikk pvvvekeeee apaeitlkrr pkekpveeit
    21841 eevteeitik kptpeekeka peevsekvev kkpkkkkpvv eeaadevtik kvvveekkee
    21901 eapeevtlkp rpkkksvtee evaevtltkp kpieeekkpe eseltikkkp kkkpvieeea
    21961 aevtikkpvp vepdeapeei tirkkkkpek kpkvteevee tavtikkara pekpteeeei
    22021 ttdvqikkkk pqkkveevaa deltikkiee veqpeeeeev qeftfkrkpp klppkpieei
    22081 yedvtlrklr pkrksrpdik eitetenvtf rprttktked veqefkisln tyeeedisms
    22141 gkvrlkpkkk pltyseetge etiriiqeve ddsgpiieei iesdeegkde ysieeldtde
    22201 mrlplrrkkk kvpkpykved ieegdvklkl krerkysiee adeslalklk skrrvstyde
    22261 eeatlsitre ediseeveie yvvrdgdtmf sicsyvaetd eainlveger vyvidhtnqd
    22321 wwfvkkhlte ekgwvpaqyl lnevhytlyl qrklhekidk lpvfekpapg dkssapkfve
    22381 klqpihtpdg ytiqfecqve gvprpqitwf rqtaiikpsp dfqmyydddn vatliirevf
    22441 pedagtftcv aknaagfass tteliveapl sdhgsdltgp srkslsress ladilegipp
    22501 tfsrkpkaky vdegedvile crlvavpepe itwyykdtqi ttkeniiiat esdmhmycsv
    22561 ikitkvqkkq egkytivarn regeatieip mkvktgksep peileplqsy vvregetvvl
    22621 stqivgnpap kvtwfkngkp lkgltpkqdg hvntltliqp qvsdtgeysv vatndlgtae
    22681 tkatltvein lkpslainfq qhtfehfsvt keipsgapep plfterfqel tvpekgtfkl
    22741 vakvtgnpvp evtwlrnnkp lekspnitet ydgenivlei rnansevdtg dykcvasnpv
    22801 gkashgarvt vdvdkvtftk tlekevvvde yktleltcet shtvstkwwh ndkeisgmdh
    22861 reiieegrvh klvikktnpt degtykctvk nqstssnvtv katkpefvkk lqdcevkerd
    22921 vaileveits qtvdvkwykd geqlgpgkek lefvkegtvr kllirstsvh degeytcsll
    22981 dqectaevtv ielppeiitk mqdvtiarge ratfdieltk gdalvrwfkd gqelqfsehv
    23041 rlsidgkrqk lkiydtesed agvyscevgq qkssakltve epavefitkl pdvtlvplns
    23101 davfvielsq dvpvtwmrkt qvikespkyt iidegkvkkl ivkkcttedi seytamvanv
    23161 ktssrlrvev ietppkispd tprkykvrkd edvdivvkfs gtpkpedewt vngrvvtksk
    23221 raiptidees avltirkiqe edvgdytlkl vnsvgetsie invvivqvps apgapeplet
    23281 tndsvtlhwk kpdsdgnspi veyilehqek tettwtkite titetthkvt klttnkeytf
    23341 rvtavneagp getsptspyv kiskpstaep pvileplksi viglgetvtl scviggtpap
    23401 eitwlrndei fedtsityen hitkytiret tetssakftv kaknnvgsae ttcelkiqes
    23461 pkiiydenlt nqklpmngqw kieiqitgfp kpevtwtkng kevdekrvsi hreertstvs
    23521 isslvredta tymakaenqa gsssvdlhlk vidkpskpqg pvvfkeirqd rvtlewrppa
    23581 ddggieleky tiekcepgkt wvkvvdidke vesfcvhklq qnaeykfrii arnavgasep
    23641 lesdtvkmrt sfeppgaprg plevsgmtkt sftikwqppe ndggtpitdy iieikeeskk
    23701 twqkigstkh etthfsvsdl ktdvpynfri taknsvgsgp pyvaeepiap grrikitklt
    23761 essmyallgi fpptkivisk tppsspqhvh vtnitsksat lswsppastg gteltgyvie
    23821 krpligkgar wtkvvtldat tlqycvenlk eseflfrifa ennqglsvpa nsepvtlkth
    23881 asvpspptap leirqiaant vviswgrpds dggaplegyk vairdtkrtm wmevarvnte
    23941 iqklnirdlq enhmylirif arneiglsdp leseepfkii paseltvvep iaeatekget
    24001 asisfstent sswlrehnmd adihsyarar llrqdeyffr iwhyakklfe
//
LOCUS       XP_034182819           24045 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034182819
VERSION     XP_034182819.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326928.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182819.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24045
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..24045
                     /product="sallimus isoform X5"
                     /calculated_mol_wt=2716762
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16500..>16652
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22306..22358
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22311,22313,22319..22320,22337..22339,22352,22354,
                     22356..22357)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22394..22484
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22411..22415
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22424..22428
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22450..22454
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22464..22469
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22477..22480
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22518..22611
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22535..22539
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22548..22552
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22573..22581
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22591..22596
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22604..22607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22619..22705
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22636..22640
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22649..22653
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22671..22675
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22685..22690
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22698..22701
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22716..22806
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22733..22737
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22746..22750
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22771..22775
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22786..22791
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22799..22802
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22819..22896
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22829..22833
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22840..22844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22865..22869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22879..22884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22900..22985
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22917..22921
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22929..22933
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22955..22959
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22969..22974
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22978..22981
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22990..23074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23007..23011
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23019..23023
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23044..23048
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23058..23063
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23067..23070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23180..23260
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23188..23192
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23201..23205
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23226..23230
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23240..23245
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23253..23256
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23264..23355
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23264,23329,23344)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23345..23346,23348..23349)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23366..23452
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23383..23387
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23396..23400
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23418..23422
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23432..23437
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23445..23448
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23476..23546
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23476..23479
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23488..23492
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23512..23516
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23526..23531
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23539..23542
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23550..23642
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23550,23616,23631)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23620..23935
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23632..23633,23635..23636)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..24045
                     /gene="sls"
                     /coded_by="XM_034326928.2:755..72892"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    13501 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    13561 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    13621 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    13681 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    13741 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    13801 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    13861 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    13921 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    13981 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    14041 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    14101 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    14161 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    14221 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    14281 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    14341 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    14401 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    14461 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    14521 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    14581 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    14641 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    14701 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    14761 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    14821 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    14881 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    14941 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    15001 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    15061 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    15121 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    15181 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    15241 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    15301 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    15361 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    15421 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    15481 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    15541 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    15601 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    15661 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    15721 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    15781 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    15841 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    15901 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    15961 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    16021 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    16081 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    16141 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    16201 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    16261 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    16321 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    16381 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    16441 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    16501 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    16561 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    16621 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    16681 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    16741 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    16801 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivetteapaq qhhititqht
    16861 tkhdlpqqqs iesdteviee yttkfrrgst edataavktk ktivrkkpkt tgeedivvvi
    16921 eeelddikpk ipsipkkqfm pieevttvsd veeivsarid eaeeivpkee ekvevtitev
    16981 qdtdkkkkvt kkqfvrrrgk trqvteeitv eeegkapett vvtkpveiie edieerpkie
    17041 keeyvveevp eektiplkpr kkltptvqqv eeqvtvteei tkegkprktt kkkvitrkgk
    17101 eqqvtevvtv eeqgkapits vtegpveevv eeiiqklpap kyakpsevee vreqvtvtee
    17161 vtrlgkpkkt tkkkiikrkg keqqvtevvt veeegkaptt tvtegpveev vieeikelpi
    17221 pefvkpteve evreqvtvte eltkmgkpkk tikkkiikrk gkeqqvtevv tveeqgkapv
    17281 ttvkegpvee vveetitpll apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    17341 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    17401 tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt vtegpveevv detvkalpap
    17461 eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    17521 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    17581 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    17641 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    17701 reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv tevvtveeqg kapvttvneg
    17761 pveevvdetv kalpapeyae pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    17821 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    17881 kktikkkiik kkgkeqqvte lvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    17941 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp
    18001 veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv
    18061 tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk
    18121 kttkkkiikr kgkeqqvtei vtveeegkap kttvmegpve evvieeiqel pipefvkpte
    18181 veevreqvtv teevtrlgkp kkttkkkiik rkgkeqqvte ivtveeqgka pvttvtegpv
    18241 eevvdetiks lpapeyakps eveevreqvt vtqevtklgk pkkttkkkiv krkgkeqqvt
    18301 eivtveeegk apkttvtegp veevveeevk pllapefvkp seveevheqv tvtqevtreg
    18361 kpmktikkki ikkkgkaqqv tevvtveeeg kvpvttvkeg pveevvdetv kalpapkyak
    18421 pseveevreq vtvtqeitkl gkpkkttkrk vikrkgkeqq vtevvtveee gkapettvte
    18481 gpveevleee ikplpgpefv kpseveevre qvtvteevtr egkpkktikk kiikkkgkeq
    18541 qvteivtvee qgkapvtsvt egpveevvde tikqlpapky akpseveevr eqvtvtqemt
    18601 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttm tegpveevve eeikplpale
    18661 fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk eqqitevvtv eeqgkapvtt
    18721 vtkapveeiv detikelpal efakpsevee vreqvtvtke vtkegkpkkt ikrkvikkkg
    18781 keqqvteivt veeegkapkt titegpveev veeeikplpa lefvkpseve evreqvtvte
    18841 evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv ttvqegpvee videtikslp
    18901 apeyakpsev eeireqvtvt eeltklgkpk kttkkkitkr kgpeqqitei vtveeegkap
    18961 vttvtegpve evvkevlkpl pvpkvvkpse veeireqvtv teemtkegkp kkiikkkvik
    19021 rkgreqtvqe vvtveekgka pvttvtvgpt eeiveetvkt lpapeyakps eveevreqvt
    19081 vtqevtkegk pkkttkkkvi krkgkeqqvt evvtveeqgk apvttvtegp veevveellk
    19141 pliapeyvkp seveevreqv tvteeltkeg kpkkvikkkv ikrrgreqqv kevvtveeqg
    19201 kapvttvteg pteeiveeti kalpapedak pseveevreq vtvtqevtke gkpkktikkk
    19261 vtkrkgkeqq vtevvtveek gkapvttvte gpveeiveet vkalpapelv kptevtevpe
    19321 eltvtevvtk egktkkivkk kvpkpkkegi eapeeapeev pveetkkiik lqrlkvskfe
    19381 stkldiqets lgkpqfaqik lrktpvakrp skkdekfpri llrsriiaie wppspknlkv
    19441 dtfepielqn gilsrnvdea aalpkpkkkk ikriekeiak lekldqefee lkkelplekl
    19501 eepileekpe rkvkkkkkpe dkakegvkpq lmrisikeqk pikpkiaepa tplfaqiklk
    19561 kapakpkkkp eeevskfpki llrsritahe wppsvkypai svlepnyiqn gilsrtmeea
    19621 lklkkvkhkk mklpekelte lekldqefee lkkvplekve eitpyerkpk pekpeeekpk
    19681 klvigkgkrr paeeedletv qlkkipekip sapeeaekvp kkkdevdkpk kekeekeype
    19741 lkpfepydie apeveleere kpeypepekp kekkvpgkkp kkerktpaqe teavpivpgv
    19801 pkpkepeeee eikrriperp ppteepekik lkpwkkgekp eeeekkpedy ikfvpkdddi
    19861 vetvevtttv eetpdkkqrk ikkkktitkp kdekpkiiee ttieeegfep vvtveefdvd
    19921 kyeavpvesv ekaapveeik eeivtseiit eegtkktvrk krvtkkregk ervteevtve
    19981 eegkepitat ielhedtise iheqakhkrp tkpkadekla edvtektveq qeiieevkev
    20041 kptkkvikke kpvkakdevt eevveeiiie epekqeakpi veeeetviit ehdvrkapkk
    20101 kkevvikeee eifetietpt hkiirkkirp mkkgekevvv eeiiqkpree tvemeeqqiv
    20161 evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk akptveeekk kvkvtkvdtk
    20221 kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii eeviqktiee akameeqeiv
    20281 evietptkkv ikkktttiee gkapeekvee iviekpkevi eeeeeiieti etptkrivrk
    20341 kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv ikkkkatike gkapeeevee
    20401 iviekpikek akkkilpkeg veitevitet sveelieeel kpeeakpsee veeekvkite
    20461 vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp tvtekegiev tevisevape
    20521 klpeeakpkk kkateikete ekvitkkvkv keapkeavpg eeeqpaeevd elvpekpkkt
    20581 kakkevtpqd sivttevvpi tkeeelevek peeekatvve ekeekakvee kkvtvkkapk
    20641 kklkpteaee eqveevveei tpeepkkrka kkavipkeei qttevipgit aeeipeeekp
    20701 eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge vieekpkvke ipeekpkeev
    20761 pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee vkpeeekpev kpeeekpkee
    20821 kpkkkkpaek kpkiseeerp edkveeipek kpeevktree iipkdtvqtt evipetvaee
    20881 iptkkpkerk avpveekeek veitevpvek apekkeekvp eekpteaape kpkeakvmkk
    20941 kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke kkplpeslqk lvpqkierke
    21001 mkptklkyse paetvqfasi klkkvavpkk keadetkfpk imlrsrmtfv gdyppelqip
    21061 kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev tqlekldlef eelkkvplek
    21121 iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe kvklkkvpek kpeeveeplk
    21181 kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek yepeeyekpe epapepvpkp
    21241 yekepkkkpd qeveqiplkk gkpkspepde epevkfript kekpeeepeq itlkpwkkek
    21301 peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk ekpkeekeee vtvkkpeeek
    21361 peevaeevtl kkepekpvee apaevtikkp vveekeveee etitevilkk kpekpeevve
    21421 eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi kkpkkksipe iteevteeit
    21481 lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv eekkeeevpe evtlkpkkpe
    21541 ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee aadeitlkkp vvvekeeeei
    21601 teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape evteeiaikk pkkkkpvkee
    21661 aadevtikkp vveekkeeet peevtlkprk pkekpvpeev keevtlkkpv peekekvpee
    21721 vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit eeitlgkkkk pekkpvpeei
    21781 teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea adevtikkpv vvekeeeeap
    21841 aeitlkrrpk ekpveeitee vteeitikkp tpeekekape evsekvevkk pkkkkpvvee
    21901 aadevtikkv vveekkeeea peevtlkprp kkksvteeev aevtltkpkp ieeekkpees
    21961 eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti rkkkkpekkp kvteeveeta
    22021 vtikkarape kpteeeeitt dvqikkkkpq kkveevaade ltikkieeve qpeeeeevqe
    22081 ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei tetenvtfrp rttktkedve
    22141 qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet iriiqevedd sgpiieeiie
    22201 sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie egdvklklkr erkysieead
    22261 eslalklksk rrvstydeee atlsitreed iseeveieyv vrdgdtmfsi csyvaetdea
    22321 inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln evhytlylqr klhekidklp
    22381 vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv prpqitwfrq taiikpspdf
    22441 qmyydddnva tliirevfpe dagtftcvak naagfasstt eliveaplsd hgsdltgpsr
    22501 kslsressla dilegipptf srkpkakyvd egedvilecr lvavpepeit wyykdtqitt
    22561 keniiiates dmhmycsvik itkvqkkqeg kytivarnre geatieipmk vktgkseppe
    22621 ileplqsyvv regetvvlst qivgnpapkv twfkngkplk gltpkqdghv ntltliqpqv
    22681 sdtgeysvva tndlgtaetk atltvekips gapepplfte rfqeltvpek gtfklvakvt
    22741 gnpvpevtwl rnnkpleksp nitetydgen ivleirnans evdtgdykcv asnpvgkash
    22801 garvtvdvdk vtftktleke vvvdeyktle ltcetshtvs tkwwhndkei sgmdhreiie
    22861 egrvhklvik ktnptdegty kctvknqsts snvtvkatkp efvkklqdce vkerdvaile
    22921 veitsqtvdv kwykdgeqlg pgkeklefvk egtvrkllir stsvhdegey tcslldqect
    22981 aevtvielpp eiitkmqdvt iargeratfd ieltkgdalv rwfkdgqelq fsehvrlsid
    23041 gkrqklkiyd tesedagvys cevgqqkssa kltveepave fitklpdvtl vplnsdavfv
    23101 ielsqdvpvt wmrktqvike spkytiideg kvkklivkkc ttediseyta mvanvktssr
    23161 lrvevietpp kispdtprky kvrkdedvdi vvkfsgtpkp edewtvngrv vtkskraipt
    23221 ideesavlti rkiqeedvgd ytlklvnsvg etsieinvvi vqvpsapgap eplettndsv
    23281 tlhwkkpdsd gnspiveyil ehqektettw tkitetitet thkvtklttn keytfrvtav
    23341 neagpgetsp tspyvkiskp staeppvile plksiviglg etvtlscvig gtpapeitwl
    23401 rndeifedts ityenhitky tirettetss akftvkaknn vgsaettcel kiqespkiiy
    23461 denltnqklp mngqwkieiq itgfpkpevt wtkngkevde krvsihreer tstvsisslv
    23521 redtatymak aenqagsssv dlhlkvidkp skpqgpvvfk eirqdrvtle wrppaddggi
    23581 elekytiekc epgktwvkvv didkevesfc vhklqqnaey kfriiarnav gaseplesdt
    23641 vkmrtsfepp gaprgplevs gmtktsftik wqppendggt pitdyiieik eeskktwqki
    23701 gstkhetthf svsdlktdvp ynfritakns vgsgppyvae epiapgrrik itkltessmy
    23761 allgifpptk ivisktppss pqhvhvtnit sksatlswsp pastggtelt gyviekrpli
    23821 gkgarwtkvv tldattlqyc venlkesefl frifaennqg lsvpansepv tlkthasvps
    23881 pptapleirq iaantvvisw grpdsdggap legykvaird tkrtmwmeva rvnteiqkln
    23941 irdlqenhmy lirifarnei glsdplesee pfkiipasel tvvepiaeat ekgetasisf
    24001 stentsswlr ehnmdadihs yararllrqd eyffriwhya kklfe
//
LOCUS       XP_034182828           24042 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034182828
VERSION     XP_034182828.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326937.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182828.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24042
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..24042
                     /product="sallimus isoform X6"
                     /calculated_mol_wt=2716583
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16500..>16652
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22306..22358
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22311,22313,22319..22320,22337..22339,22352,22354,
                     22356..22357)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22394..22484
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22411..22415
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22424..22428
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22450..22454
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22464..22469
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22477..22480
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22518..22611
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22535..22539
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22548..22552
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22573..22581
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22591..22596
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22604..22607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22619..22705
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22636..22640
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22649..22653
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22671..22675
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22685..22690
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22698..22701
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22739..22829
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22756..22760
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22769..22773
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22794..22798
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22809..22814
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22822..22825
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22842..22919
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22852..22856
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22863..22867
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22888..22892
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22902..22907
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22923..23008
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22940..22944
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22952..22956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22978..22982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22992..22997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23001..23004
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23013..23097
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23030..23034
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23042..23046
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23067..23071
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23081..23086
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23090..23093
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23203..23283
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23211..23215
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23224..23228
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23249..23253
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23263..23268
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23276..23279
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23287..23378
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23287,23352,23367)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23368..23369,23371..23372)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23389..23475
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23406..23410
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23419..23423
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23441..23445
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23455..23460
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23468..23471
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23499..23569
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23499..23502
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23511..23515
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23535..23539
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23549..23554
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23562..23565
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23573..23665
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23573,23639,23654)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23643..23932
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23655..23656,23658..23659)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..24042
                     /gene="sls"
                     /coded_by="XM_034326937.2:755..72883"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    13501 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    13561 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    13621 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    13681 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    13741 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    13801 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    13861 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    13921 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    13981 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    14041 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    14101 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    14161 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    14221 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    14281 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    14341 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    14401 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    14461 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    14521 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    14581 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    14641 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    14701 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    14761 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    14821 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    14881 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    14941 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    15001 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    15061 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    15121 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    15181 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    15241 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    15301 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    15361 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    15421 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    15481 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    15541 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    15601 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    15661 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    15721 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    15781 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    15841 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    15901 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    15961 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    16021 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    16081 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    16141 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    16201 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    16261 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    16321 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    16381 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    16441 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    16501 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    16561 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    16621 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    16681 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    16741 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    16801 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivetteapaq qhhititqht
    16861 tkhdlpqqqs iesdteviee yttkfrrgst edataavktk ktivrkkpkt tgeedivvvi
    16921 eeelddikpk ipsipkkqfm pieevttvsd veeivsarid eaeeivpkee ekvevtitev
    16981 qdtdkkkkvt kkqfvrrrgk trqvteeitv eeegkapett vvtkpveiie edieerpkie
    17041 keeyvveevp eektiplkpr kkltptvqqv eeqvtvteei tkegkprktt kkkvitrkgk
    17101 eqqvtevvtv eeqgkapits vtegpveevv eeiiqklpap kyakpsevee vreqvtvtee
    17161 vtrlgkpkkt tkkkiikrkg keqqvtevvt veeegkaptt tvtegpveev vieeikelpi
    17221 pefvkpteve evreqvtvte eltkmgkpkk tikkkiikrk gkeqqvtevv tveeqgkapv
    17281 ttvkegpvee vveetitpll apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    17341 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    17401 tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt vtegpveevv detvkalpap
    17461 eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    17521 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    17581 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    17641 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    17701 reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv tevvtveeqg kapvttvneg
    17761 pveevvdetv kalpapeyae pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    17821 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    17881 kktikkkiik kkgkeqqvte lvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    17941 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp
    18001 veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv
    18061 tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk
    18121 kttkkkiikr kgkeqqvtei vtveeegkap kttvmegpve evvieeiqel pipefvkpte
    18181 veevreqvtv teevtrlgkp kkttkkkiik rkgkeqqvte ivtveeqgka pvttvtegpv
    18241 eevvdetiks lpapeyakps eveevreqvt vtqevtklgk pkkttkkkiv krkgkeqqvt
    18301 eivtveeegk apkttvtegp veevveeevk pllapefvkp seveevheqv tvtqevtreg
    18361 kpmktikkki ikkkgkaqqv tevvtveeeg kvpvttvkeg pveevvdetv kalpapkyak
    18421 pseveevreq vtvtqeitkl gkpkkttkrk vikrkgkeqq vtevvtveee gkapettvte
    18481 gpveevleee ikplpgpefv kpseveevre qvtvteevtr egkpkktikk kiikkkgkeq
    18541 qvteivtvee qgkapvtsvt egpveevvde tikqlpapky akpseveevr eqvtvtqemt
    18601 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttm tegpveevve eeikplpale
    18661 fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk eqqitevvtv eeqgkapvtt
    18721 vtkapveeiv detikelpal efakpsevee vreqvtvtke vtkegkpkkt ikrkvikkkg
    18781 keqqvteivt veeegkapkt titegpveev veeeikplpa lefvkpseve evreqvtvte
    18841 evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv ttvqegpvee videtikslp
    18901 apeyakpsev eeireqvtvt eeltklgkpk kttkkkitkr kgpeqqitei vtveeegkap
    18961 vttvtegpve evvkevlkpl pvpkvvkpse veeireqvtv teemtkegkp kkiikkkvik
    19021 rkgreqtvqe vvtveekgka pvttvtvgpt eeiveetvkt lpapeyakps eveevreqvt
    19081 vtqevtkegk pkkttkkkvi krkgkeqqvt evvtveeqgk apvttvtegp veevveellk
    19141 pliapeyvkp seveevreqv tvteeltkeg kpkkvikkkv ikrrgreqqv kevvtveeqg
    19201 kapvttvteg pteeiveeti kalpapedak pseveevreq vtvtqevtke gkpkktikkk
    19261 vtkrkgkeqq vtevvtveek gkapvttvte gpveeiveet vkalpapelv kptevtevpe
    19321 eltvtevvtk egktkkivkk kvpkpkkegi eapeeapeev pveetkkiik lqrlkvskfe
    19381 stkldiqets lgkpqfaqik lrktpvakrp skkdekfpri llrsriiaie wppspknlkv
    19441 dtfepielqn gilsrnvdea aalpkpkkkk ikriekeiak lekldqefee lkkelplekl
    19501 eepileekpe rkvkkkkkpe dkakegvkpq lmrisikeqk pikpkiaepa tplfaqiklk
    19561 kapakpkkkp eeevskfpki llrsritahe wppsvkypai svlepnyiqn gilsrtmeea
    19621 lklkkvkhkk mklpekelte lekldqefee lkkvplekve eitpyerkpk pekpeeekpk
    19681 klvigkgkrr paeeedletv qlkkipekip sapeeaekvp kkkdevdkpk kekeekeype
    19741 lkpfepydie apeveleere kpeypepekp kekkvpgkkp kkerktpaqe teavpivpgv
    19801 pkpkepeeee eikrriperp ppteepekik lkpwkkgekp eeeekkpedy ikfvpkdddi
    19861 vetvevtttv eetpdkkqrk ikkkktitkp kdekpkiiee ttieeegfep vvtveefdvd
    19921 kyeavpvesv ekaapveeik eeivtseiit eegtkktvrk krvtkkregk ervteevtve
    19981 eegkepitat ielhedtise iheqakhkrp tkpkadekla edvtektveq qeiieevkev
    20041 kptkkvikke kpvkakdevt eevveeiiie epekqeakpi veeeetviit ehdvrkapkk
    20101 kkevvikeee eifetietpt hkiirkkirp mkkgekevvv eeiiqkpree tvemeeqqiv
    20161 evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk akptveeekk kvkvtkvdtk
    20221 kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii eeviqktiee akameeqeiv
    20281 evietptkkv ikkktttiee gkapeekvee iviekpkevi eeeeeiieti etptkrivrk
    20341 kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv ikkkkatike gkapeeevee
    20401 iviekpikek akkkilpkeg veitevitet sveelieeel kpeeakpsee veeekvkite
    20461 vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp tvtekegiev tevisevape
    20521 klpeeakpkk kkateikete ekvitkkvkv keapkeavpg eeeqpaeevd elvpekpkkt
    20581 kakkevtpqd sivttevvpi tkeeelevek peeekatvve ekeekakvee kkvtvkkapk
    20641 kklkpteaee eqveevveei tpeepkkrka kkavipkeei qttevipgit aeeipeeekp
    20701 eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge vieekpkvke ipeekpkeev
    20761 pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee vkpeeekpev kpeeekpkee
    20821 kpkkkkpaek kpkiseeerp edkveeipek kpeevktree iipkdtvqtt evipetvaee
    20881 iptkkpkerk avpveekeek veitevpvek apekkeekvp eekpteaape kpkeakvmkk
    20941 kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke kkplpeslqk lvpqkierke
    21001 mkptklkyse paetvqfasi klkkvavpkk keadetkfpk imlrsrmtfv gdyppelqip
    21061 kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev tqlekldlef eelkkvplek
    21121 iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe kvklkkvpek kpeeveeplk
    21181 kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek yepeeyekpe epapepvpkp
    21241 yekepkkkpd qeveqiplkk gkpkspepde epevkfript kekpeeepeq itlkpwkkek
    21301 peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk ekpkeekeee vtvkkpeeek
    21361 peevaeevtl kkepekpvee apaevtikkp vveekeveee etitevilkk kpekpeevve
    21421 eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi kkpkkksipe iteevteeit
    21481 lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv eekkeeevpe evtlkpkkpe
    21541 ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee aadeitlkkp vvvekeeeei
    21601 teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape evteeiaikk pkkkkpvkee
    21661 aadevtikkp vveekkeeet peevtlkprk pkekpvpeev keevtlkkpv peekekvpee
    21721 vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit eeitlgkkkk pekkpvpeei
    21781 teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea adevtikkpv vvekeeeeap
    21841 aeitlkrrpk ekpveeitee vteeitikkp tpeekekape evsekvevkk pkkkkpvvee
    21901 aadevtikkv vveekkeeea peevtlkprp kkksvteeev aevtltkpkp ieeekkpees
    21961 eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti rkkkkpekkp kvteeveeta
    22021 vtikkarape kpteeeeitt dvqikkkkpq kkveevaade ltikkieeve qpeeeeevqe
    22081 ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei tetenvtfrp rttktkedve
    22141 qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet iriiqevedd sgpiieeiie
    22201 sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie egdvklklkr erkysieead
    22261 eslalklksk rrvstydeee atlsitreed iseeveieyv vrdgdtmfsi csyvaetdea
    22321 inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln evhytlylqr klhekidklp
    22381 vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv prpqitwfrq taiikpspdf
    22441 qmyydddnva tliirevfpe dagtftcvak naagfasstt eliveaplsd hgsdltgpsr
    22501 kslsressla dilegipptf srkpkakyvd egedvilecr lvavpepeit wyykdtqitt
    22561 keniiiates dmhmycsvik itkvqkkqeg kytivarnre geatieipmk vktgkseppe
    22621 ileplqsyvv regetvvlst qivgnpapkv twfkngkplk gltpkqdghv ntltliqpqv
    22681 sdtgeysvva tndlgtaetk atltveinlk pslainfqqh tfehfsvtke ipsgapeppl
    22741 fterfqeltv pekgtfklva kvtgnpvpev twlrnnkple kspnitetyd genivleirn
    22801 ansevdtgdy kcvasnpvgk ashgarvtvd vdkvtftktl ekevvvdeyk tleltcetsh
    22861 tvstkwwhnd keisgmdhre iieegrvhkl vikktnptde gtykctvknq stssnvtvka
    22921 tkpefvkklq dcevkerdva ileveitsqt vdvkwykdge qlgpgkekle fvkegtvrkl
    22981 lirstsvhde geytcslldq ectaevtvie lppeiitkmq dvtiargera tfdieltkgd
    23041 alvrwfkdgq elqfsehvrl sidgkrqklk iydtesedag vyscevgqqk ssakltveep
    23101 avefitklpd vtlvplnsda vfvielsqdv pvtwmrktqv ikespkytii degkvkkliv
    23161 kkcttedise ytamvanvkt ssrlrvevie tppkispdtp rkykvrkded vdivvkfsgt
    23221 pkpedewtvn grvvtkskra iptideesav ltirkiqeed vgdytlklvn svgetsiein
    23281 vvivqvpsap gapeplettn dsvtlhwkkp dsdgnspive yilehqekte ttwtkiteti
    23341 tetthkvtkl ttnkeytfrv tavneagpge tsptspyvki skpstaeppv ileplksivi
    23401 glgetvtlsc viggtpapei twlrndeife dtsityenhi tkytirette tssakftvka
    23461 knnvgsaett celkiqespk iiydenltnq klpmngqwki eiqitgfpkp evtwtkngke
    23521 vdekrvsihr eertstvsis slvredtaty makaenqags ssvdlhlkvi dkpskpqgpv
    23581 vfkeirqdrv tlewrppadd ggielekyti ekcepgktwv kvvdidkeve sfcvhklqqn
    23641 aeykfriiar navgaseple sdtvkmrtsf eppgaprgpl evsgmtktsf tikwqppend
    23701 ggtpitdyii eikeeskktw qkigstkhet thfsvsdlkt dvpynfrita knsvgsgppy
    23761 vaeepiapgr ritppsspqh vhvtnitsks atlswsppas tggteltgyv iekrpligkg
    23821 arwtkvvtld attlqycven lkeseflfri faennqglsv pansepvtlk thasvpsppt
    23881 apleirqiaa ntvviswgrp dsdggapleg ykvairdtkr tmwmevarvn teiqklnird
    23941 lqenhmylir ifarneigls dpleseepfk iipaseltvv epiaeatekg etasisfste
    24001 ntsswlrehn mdadihsyar arllrqdeyf friwhyakkl fe
//
LOCUS       XP_076546011           24019 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546011
VERSION     XP_076546011.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689896.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24019
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..24019
                     /product="sallimus isoform X7"
                     /calculated_mol_wt=2713900
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16500..>16652
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22306..22358
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22311,22313,22319..22320,22337..22339,22352,22354,
                     22356..22357)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22394..22484
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22411..22415
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22424..22428
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22450..22454
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22464..22469
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22477..22480
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22518..22611
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22535..22539
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22548..22552
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22573..22581
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22591..22596
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22604..22607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22619..22705
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22636..22640
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22649..22653
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22671..22675
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22685..22690
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22698..22701
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22716..22806
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22733..22737
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22746..22750
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22771..22775
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22786..22791
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22799..22802
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22819..22896
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22829..22833
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22840..22844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22865..22869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22879..22884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22900..22985
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22917..22921
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22929..22933
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22955..22959
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22969..22974
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22978..22981
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22990..23074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23007..23011
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23019..23023
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23044..23048
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23058..23063
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23067..23070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23180..23260
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23188..23192
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23201..23205
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23226..23230
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23240..23245
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23253..23256
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23264..23355
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23264,23329,23344)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23345..23346,23348..23349)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23366..23452
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23383..23387
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23396..23400
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23418..23422
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23432..23437
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23445..23448
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23476..23546
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23476..23479
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23488..23492
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23512..23516
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23526..23531
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23539..23542
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23550..23642
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23550,23616,23631)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23620..23909
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23632..23633,23635..23636)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..24019
                     /gene="sls"
                     /coded_by="XM_076689896.1:755..72814"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    13501 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    13561 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    13621 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    13681 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    13741 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    13801 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    13861 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    13921 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    13981 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    14041 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    14101 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    14161 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    14221 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    14281 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    14341 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    14401 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    14461 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    14521 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    14581 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    14641 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    14701 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    14761 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    14821 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    14881 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    14941 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    15001 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    15061 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    15121 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    15181 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    15241 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    15301 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    15361 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    15421 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    15481 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    15541 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    15601 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    15661 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    15721 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    15781 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    15841 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    15901 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    15961 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    16021 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    16081 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    16141 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    16201 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    16261 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    16321 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    16381 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    16441 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    16501 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    16561 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    16621 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    16681 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    16741 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    16801 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivetteapaq qhhititqht
    16861 tkhdlpqqqs iesdteviee yttkfrrgst edataavktk ktivrkkpkt tgeedivvvi
    16921 eeelddikpk ipsipkkqfm pieevttvsd veeivsarid eaeeivpkee ekvevtitev
    16981 qdtdkkkkvt kkqfvrrrgk trqvteeitv eeegkapett vvtkpveiie edieerpkie
    17041 keeyvveevp eektiplkpr kkltptvqqv eeqvtvteei tkegkprktt kkkvitrkgk
    17101 eqqvtevvtv eeqgkapits vtegpveevv eeiiqklpap kyakpsevee vreqvtvtee
    17161 vtrlgkpkkt tkkkiikrkg keqqvtevvt veeegkaptt tvtegpveev vieeikelpi
    17221 pefvkpteve evreqvtvte eltkmgkpkk tikkkiikrk gkeqqvtevv tveeqgkapv
    17281 ttvkegpvee vveetitpll apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    17341 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    17401 tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt vtegpveevv detvkalpap
    17461 eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    17521 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    17581 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    17641 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    17701 reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv tevvtveeqg kapvttvneg
    17761 pveevvdetv kalpapeyae pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    17821 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    17881 kktikkkiik kkgkeqqvte lvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    17941 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp
    18001 veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv
    18061 tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk
    18121 kttkkkiikr kgkeqqvtei vtveeegkap kttvmegpve evvieeiqel pipefvkpte
    18181 veevreqvtv teevtrlgkp kkttkkkiik rkgkeqqvte ivtveeqgka pvttvtegpv
    18241 eevvdetiks lpapeyakps eveevreqvt vtqevtklgk pkkttkkkiv krkgkeqqvt
    18301 eivtveeegk apkttvtegp veevveeevk pllapefvkp seveevheqv tvtqevtreg
    18361 kpmktikkki ikkkgkaqqv tevvtveeeg kvpvttvkeg pveevvdetv kalpapkyak
    18421 pseveevreq vtvtqeitkl gkpkkttkrk vikrkgkeqq vtevvtveee gkapettvte
    18481 gpveevleee ikplpgpefv kpseveevre qvtvteevtr egkpkktikk kiikkkgkeq
    18541 qvteivtvee qgkapvtsvt egpveevvde tikqlpapky akpseveevr eqvtvtqemt
    18601 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttm tegpveevve eeikplpale
    18661 fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk eqqitevvtv eeqgkapvtt
    18721 vtkapveeiv detikelpal efakpsevee vreqvtvtke vtkegkpkkt ikrkvikkkg
    18781 keqqvteivt veeegkapkt titegpveev veeeikplpa lefvkpseve evreqvtvte
    18841 evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv ttvqegpvee videtikslp
    18901 apeyakpsev eeireqvtvt eeltklgkpk kttkkkitkr kgpeqqitei vtveeegkap
    18961 vttvtegpve evvkevlkpl pvpkvvkpse veeireqvtv teemtkegkp kkiikkkvik
    19021 rkgreqtvqe vvtveekgka pvttvtvgpt eeiveetvkt lpapeyakps eveevreqvt
    19081 vtqevtkegk pkkttkkkvi krkgkeqqvt evvtveeqgk apvttvtegp veevveellk
    19141 pliapeyvkp seveevreqv tvteeltkeg kpkkvikkkv ikrrgreqqv kevvtveeqg
    19201 kapvttvteg pteeiveeti kalpapedak pseveevreq vtvtqevtke gkpkktikkk
    19261 vtkrkgkeqq vtevvtveek gkapvttvte gpveeiveet vkalpapelv kptevtevpe
    19321 eltvtevvtk egktkkivkk kvpkpkkegi eapeeapeev pveetkkiik lqrlkvskfe
    19381 stkldiqets lgkpqfaqik lrktpvakrp skkdekfpri llrsriiaie wppspknlkv
    19441 dtfepielqn gilsrnvdea aalpkpkkkk ikriekeiak lekldqefee lkkelplekl
    19501 eepileekpe rkvkkkkkpe dkakegvkpq lmrisikeqk pikpkiaepa tplfaqiklk
    19561 kapakpkkkp eeevskfpki llrsritahe wppsvkypai svlepnyiqn gilsrtmeea
    19621 lklkkvkhkk mklpekelte lekldqefee lkkvplekve eitpyerkpk pekpeeekpk
    19681 klvigkgkrr paeeedletv qlkkipekip sapeeaekvp kkkdevdkpk kekeekeype
    19741 lkpfepydie apeveleere kpeypepekp kekkvpgkkp kkerktpaqe teavpivpgv
    19801 pkpkepeeee eikrriperp ppteepekik lkpwkkgekp eeeekkpedy ikfvpkdddi
    19861 vetvevtttv eetpdkkqrk ikkkktitkp kdekpkiiee ttieeegfep vvtveefdvd
    19921 kyeavpvesv ekaapveeik eeivtseiit eegtkktvrk krvtkkregk ervteevtve
    19981 eegkepitat ielhedtise iheqakhkrp tkpkadekla edvtektveq qeiieevkev
    20041 kptkkvikke kpvkakdevt eevveeiiie epekqeakpi veeeetviit ehdvrkapkk
    20101 kkevvikeee eifetietpt hkiirkkirp mkkgekevvv eeiiqkpree tvemeeqqiv
    20161 evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk akptveeekk kvkvtkvdtk
    20221 kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii eeviqktiee akameeqeiv
    20281 evietptkkv ikkktttiee gkapeekvee iviekpkevi eeeeeiieti etptkrivrk
    20341 kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv ikkkkatike gkapeeevee
    20401 iviekpikek akkkilpkeg veitevitet sveelieeel kpeeakpsee veeekvkite
    20461 vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp tvtekegiev tevisevape
    20521 klpeeakpkk kkateikete ekvitkkvkv keapkeavpg eeeqpaeevd elvpekpkkt
    20581 kakkevtpqd sivttevvpi tkeeelevek peeekatvve ekeekakvee kkvtvkkapk
    20641 kklkpteaee eqveevveei tpeepkkrka kkavipkeei qttevipgit aeeipeeekp
    20701 eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge vieekpkvke ipeekpkeev
    20761 pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee vkpeeekpev kpeeekpkee
    20821 kpkkkkpaek kpkiseeerp edkveeipek kpeevktree iipkdtvqtt evipetvaee
    20881 iptkkpkerk avpveekeek veitevpvek apekkeekvp eekpteaape kpkeakvmkk
    20941 kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke kkplpeslqk lvpqkierke
    21001 mkptklkyse paetvqfasi klkkvavpkk keadetkfpk imlrsrmtfv gdyppelqip
    21061 kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev tqlekldlef eelkkvplek
    21121 iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe kvklkkvpek kpeeveeplk
    21181 kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek yepeeyekpe epapepvpkp
    21241 yekepkkkpd qeveqiplkk gkpkspepde epevkfript kekpeeepeq itlkpwkkek
    21301 peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk ekpkeekeee vtvkkpeeek
    21361 peevaeevtl kkepekpvee apaevtikkp vveekeveee etitevilkk kpekpeevve
    21421 eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi kkpkkksipe iteevteeit
    21481 lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv eekkeeevpe evtlkpkkpe
    21541 ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee aadeitlkkp vvvekeeeei
    21601 teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape evteeiaikk pkkkkpvkee
    21661 aadevtikkp vveekkeeet peevtlkprk pkekpvpeev keevtlkkpv peekekvpee
    21721 vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit eeitlgkkkk pekkpvpeei
    21781 teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea adevtikkpv vvekeeeeap
    21841 aeitlkrrpk ekpveeitee vteeitikkp tpeekekape evsekvevkk pkkkkpvvee
    21901 aadevtikkv vveekkeeea peevtlkprp kkksvteeev aevtltkpkp ieeekkpees
    21961 eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti rkkkkpekkp kvteeveeta
    22021 vtikkarape kpteeeeitt dvqikkkkpq kkveevaade ltikkieeve qpeeeeevqe
    22081 ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei tetenvtfrp rttktkedve
    22141 qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet iriiqevedd sgpiieeiie
    22201 sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie egdvklklkr erkysieead
    22261 eslalklksk rrvstydeee atlsitreed iseeveieyv vrdgdtmfsi csyvaetdea
    22321 inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln evhytlylqr klhekidklp
    22381 vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv prpqitwfrq taiikpspdf
    22441 qmyydddnva tliirevfpe dagtftcvak naagfasstt eliveaplsd hgsdltgpsr
    22501 kslsressla dilegipptf srkpkakyvd egedvilecr lvavpepeit wyykdtqitt
    22561 keniiiates dmhmycsvik itkvqkkqeg kytivarnre geatieipmk vktgkseppe
    22621 ileplqsyvv regetvvlst qivgnpapkv twfkngkplk gltpkqdghv ntltliqpqv
    22681 sdtgeysvva tndlgtaetk atltvekips gapepplfte rfqeltvpek gtfklvakvt
    22741 gnpvpevtwl rnnkpleksp nitetydgen ivleirnans evdtgdykcv asnpvgkash
    22801 garvtvdvdk vtftktleke vvvdeyktle ltcetshtvs tkwwhndkei sgmdhreiie
    22861 egrvhklvik ktnptdegty kctvknqsts snvtvkatkp efvkklqdce vkerdvaile
    22921 veitsqtvdv kwykdgeqlg pgkeklefvk egtvrkllir stsvhdegey tcslldqect
    22981 aevtvielpp eiitkmqdvt iargeratfd ieltkgdalv rwfkdgqelq fsehvrlsid
    23041 gkrqklkiyd tesedagvys cevgqqkssa kltveepave fitklpdvtl vplnsdavfv
    23101 ielsqdvpvt wmrktqvike spkytiideg kvkklivkkc ttediseyta mvanvktssr
    23161 lrvevietpp kispdtprky kvrkdedvdi vvkfsgtpkp edewtvngrv vtkskraipt
    23221 ideesavlti rkiqeedvgd ytlklvnsvg etsieinvvi vqvpsapgap eplettndsv
    23281 tlhwkkpdsd gnspiveyil ehqektettw tkitetitet thkvtklttn keytfrvtav
    23341 neagpgetsp tspyvkiskp staeppvile plksiviglg etvtlscvig gtpapeitwl
    23401 rndeifedts ityenhitky tirettetss akftvkaknn vgsaettcel kiqespkiiy
    23461 denltnqklp mngqwkieiq itgfpkpevt wtkngkevde krvsihreer tstvsisslv
    23521 redtatymak aenqagsssv dlhlkvidkp skpqgpvvfk eirqdrvtle wrppaddggi
    23581 elekytiekc epgktwvkvv didkevesfc vhklqqnaey kfriiarnav gaseplesdt
    23641 vkmrtsfepp gaprgplevs gmtktsftik wqppendggt pitdyiieik eeskktwqki
    23701 gstkhetthf svsdlktdvp ynfritakns vgsgppyvae epiapgrrit ppsspqhvhv
    23761 tnitsksatl swsppastgg teltgyviek rpligkgarw tkvvtldatt lqycvenlke
    23821 seflfrifae nnqglsvpan sepvtlktha svpspptapl eirqiaantv viswgrpdsd
    23881 ggaplegykv airdtkrtmw mevarvntei qklnirdlqe nhmylirifa rneiglsdpl
    23941 eseepfkiip aseltvvepi aeatekgeta sisfstents swlrehnmda dihsyararl
    24001 lrqdeyffri whyakklfe
//
LOCUS       XP_034182837           23807 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034182837
VERSION     XP_034182837.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326946.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182837.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..23807
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..23807
                     /product="sallimus isoform X8"
                     /calculated_mol_wt=2689805
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4272
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4724..4813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4741..4745
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4754..4758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4779..4783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4793..4798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4806..4809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4970..5057
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4987..4991
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5000..5004
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5027..5031
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5041..5046
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5167..5256
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5184..5188
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5197..5201
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5222..5226
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5236..5241
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5249..5252
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5292..5382
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5309..5313
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5322..5326
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5348..5352
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5362..5367
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5375..5378
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5391..5481
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5408..5412
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5421..5425
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5447..5451
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5461..5466
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5474..5477
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5538..5629
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5555..5559
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5570..5574
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5596..5599
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5614
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5622..5625
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5671..5763
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5689..5693
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5704..5708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5729..5733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5743..5748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5756..5759
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5810..5902
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5828..5832
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5843..5847
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5868..5872
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5882..5887
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5895..5898
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5944..6036
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5962..5966
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5977..5981
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6003..6007
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6016..6021
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6029..6032
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6078..6170
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6096..6100
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6111..6115
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6136..6140
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6150..6155
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6163..6166
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6212..6303
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6229..6233
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6244..6248
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6270..6273
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6283..6288
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6296..6299
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6345..6435
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6363..6367
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6378..6382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6403..6407
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6417..6422
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6430..6433
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6479..6570
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6496..6500
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6507..6515
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6536..6540
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6550..6555
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6563..6566
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6612..6703
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6629..6633
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6644..6648
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6669..6673
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6683..6688
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6696..6699
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6745..6837
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6763..6767
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6778..6782
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6803..6807
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6817..6822
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6830..6833
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6879..6971
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6897..6901
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6910..6916
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6937..6941
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6951..6956
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6964..6967
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7011..7103
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7029..7033
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7042..7048
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7069..7073
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7083..7088
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7096..7099
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7144..7236
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7162..7166
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7175..7181
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7202..7206
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7216..7221
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7229..7232
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7278..7370
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7296..7300
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7311..7315
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7336..7340
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7350..7355
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7363..7366
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7410..7502
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7443..7447
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7468..7472
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7482..7487
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7544..7636
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7562..7566
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7577..7581
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7602..7606
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7616..7621
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7629..7632
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7676..7766
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7693..7697
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7708..7712
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7733..7737
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7747..7752
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7760..7763
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7843..7903
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7847..7851
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7872..7876
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7886..7891
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7900..7903
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7951..8042
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7984..7988
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8009..8013
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8023..8028
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8052..8143
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8070..8074
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8085..8089
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8107..8114
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8124..8129
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8137..8140
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8185..8276
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8203..8207
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8218..8222
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8240..8244
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8257..8262
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8273
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8323..8414
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8356..8360
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8381..8385
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8395..8400
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8461..8552
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8478..8482
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8493..8497
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8522
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8532..8537
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8545..8548
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8598..8688
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8615..8619
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8630..8634
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8655..8659
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8669..8674
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8682..8685
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8731..8820
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8748..8752
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8763..8767
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8788..8792
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8802..8807
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8815..8818
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8874..8964
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8892..8896
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8907..8911
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8929..8933
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8946..8951
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8959..8962
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9012..9100
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9029..9033
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9042..9046
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9066..9070
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9080..9085
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9093..9096
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9335..9425
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9352..9356
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9365..9369
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9390..9394
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9404..9409
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9417..9420
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9439..9528
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9456..9460
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9469..9473
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9494..9498
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9508..9513
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9521..9524
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9549..9638
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9566..9570
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9579..9583
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9604..9608
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9618..9623
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9631..9634
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9650..9737
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9664..9668
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9682
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9703..9707
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9717..9722
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9733
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <9854..10507
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10560..10713
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          11988..12077
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12005..12009
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12018..12022
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12043..12047
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12057..12062
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12087..12178
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12104..12108
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12117..12121
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12144..12148
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12158..12163
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12171..12174
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12193..12282
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12210..12214
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12223..12227
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12248..12252
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12262..12267
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12277..12280
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12295..12381
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12312..12316
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12325..12329
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12350..12354
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12364..12369
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12377..12380
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12398..12488
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12415..12419
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12428..12432
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12458
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12468..12473
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12481..12484
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12536..12622
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12555..12559
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12568..12572
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12594..12598
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12608..12613
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12660..12751
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12677..12681
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12690..12694
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12717..12721
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12731..12736
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12744..12747
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16239..>16391
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          22045..22097
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(22050,22052,22058..22059,22076..22078,22091,22093,
                     22095..22096)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          22133..22223
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22150..22154
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22163..22167
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22189..22193
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22203..22208
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22216..22219
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22257..22350
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22274..22278
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22287..22291
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22312..22320
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22330..22335
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22343..22346
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22358..22444
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22375..22379
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22388..22392
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22410..22414
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22424..22429
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22437..22440
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22478..22568
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22495..22499
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22508..22512
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22533..22537
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22548..22553
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22561..22564
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22581..22658
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22591..22595
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22602..22606
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22627..22631
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22641..22646
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22662..22747
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22679..22683
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22691..22695
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22717..22721
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22731..22736
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22740..22743
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22752..22836
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22769..22773
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22781..22785
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22806..22810
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22820..22825
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22829..22832
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22942..23022
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22950..22954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22963..22967
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22988..22992
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23002..23007
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23015..23018
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23026..23117
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23026,23091,23106)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(23107..23108,23110..23111)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          23128..23214
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          23145..23149
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23158..23162
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23180..23184
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23194..23199
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23207..23210
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23238..23308
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23238..23241
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23250..23254
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23274..23278
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23288..23293
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23301..23304
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23312..23404
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23312,23378,23393)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23382..23697
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23394..23395,23397..23398)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..23807
                     /gene="sls"
                     /coded_by="XM_034326946.2:755..72178"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pdvasrhmsd mtssfkskte kfvtdiqsdl kqgkpilkqp
     4501 vdgrasdade prtyreesrl sehgtkqidp dtgliyfkyd fgyefgivlp gegkktvsst
     4561 srsvqgqrra sdievpivhe fttrkengfa krsassssta rqgkpagkfs gsktvkwept
     4621 seseysesed irnarkrhse ggntmtapsl vipcspspsr wdhttpspls lspslpslsp
     4681 ryssatgpps nvdsagspwp ttngvasske viqpyleilp kkaplfitpl rdiavvsgqt
     4741 arfecivqae pqpnilwskd griienstsy eihyrngvcr ltivrafped agtyactatn
     4801 slgstvtsat llvpgvpspf ygtyrdqnls ersaesppky valrdlflpq esdvktassn
     4861 rqqqhnsstf eyawsvnrsy aggryreken ateecveits rnpiarrley sdfqandsfr
     4921 dvadfcfdre ystcnpsias prntvqgeiv mqttptvpaf tgrpgdpspp vfeqifknar
     4981 faqggnaife grvrgnpkpt vtwthkgapl leswkirmsy ddktgavtlq inqigpgdeg
     5041 eytcsaknqy geaicsvyiq pegfgpppqq qiggyrkeya qtfqsteqks qtgsqsfqqr
     5101 syqqtidkrs yvngtstsie dfkvdtfeyr llretefres itrrfvgesd vqistvvdrs
     5161 lgpvappqis qkprnsklie gsdavftaki sgnpkprltw fkngqrires qrvemshsnq
     5221 qatlrirval pedsghytll senpqgctvs saylaiessd qvdqayqtpr esvktpqves
     5281 tgesdsgkvl ppnfvrtctd rdategkmtr fdcrvtgrpy pevtwyingq qvandlthki
     5341 lvnesgnnsl mitnvsrada gvvtcvarnk agetsfqcnl nviekeqvva pkfverfttt
     5401 nvkegepvvf maravgtpvp ritwqkdgvp itpgpdvris tdgsgastld ipcaklsdaa
     5461 wyqctaqnva gstatrarlf vetpkgaape prrlnlprpt kviepepapg peviylrhve
     5521 rakpylpppe edrvyppprf iiplrdvhqi eggrihfear iepvgdptmr vewyvngral
     5581 dassratsif rfgfisldli siilqdsgey vcrvvsstgv aesratlsvt pratieqasq
     5641 hpdslqyiqq ledyskyqrq esveeissqr pafirplqdl gelqegrnah feaqltpvsd
     5701 ptmkvewykd grpitassri ttifnfgyvs lnilhlraed agsytvravn rlgeavssas
     5761 lrvyartsvt tdlgipeqqr yieaaeelea yqqamhqkyv qeqpeptspp efkspikdqn
     5821 sireggfahf earlepvgds dlrvewlkdg rpveassrit tffnfgyval tikyvtihdv
     5881 giytcraynr ageahttaql sviskndviy dsqhptglqk iqtledssry srqvqeetqv
     5941 tqaprflgtl kgtnkivegq rahfearvep qsdltmtiew yhngkaitaa nriqtyhdfg
     6001 yvaidilqvr pedagtytvv arnalgearl satmvvetrs svdtssmhrt syektqrlee
     6061 skfvepqyhi eeiskskpif vqplsdpkpv segknihlec rlepmgdptm rvewfqngrp
     6121 vtvgsrfrty ydfgfvaldi vhstildsge ytvratnhlg tahtsacvrv igksdivtet
     6181 qheqsleqiq mledaaryrk tqqeevtvmq apqftrplhn ietveltnvh lecrlqpvgd
     6241 atmkvewfvn grpvktghrf rpsyefdyva ldilgvyped sgvytcqarn qlgeavtscs
     6301 vrvhakkdll lesqhpegle riqyledasr ykrhelvdev vkvkprfvta pknqenlheg
     6361 qhahfeckle pvtdsnlkve wykngrpvti ghrfrpihdf gyvaldvidl iaedsgtytc
     6421 ravnlvgsde vscslscrst aqvltdtkne qgleqihyle drskyqrred veetttqapi
     6481 fttslnnvei kegqrahfec rlipvsdatm kvewfhnnkp vkagsrfiet nsfgfvaldi
     6541 myaypedsgt ytcrakniig eaitsatanv hskksiytet qneetlqrlh hledtsryqr
     6601 kttteeivtq apvftmpikd lkvaenqaah fearvipvgd sklkvewlrn gvpiaasnrv
     6661 ttmhdfgyva lnmkyvnped sgtytcravn dlgeavtsat lfvqskaalq fesqhesals
     6721 klqaledtsr yqrheeeeiv vkekpsftvq lngptnlveg qsahyecrie pypdpnmkve
     6781 wfhngkplst ghryrttcdf gfasldvltv yaedsgtytc katnklgsaq ssikldvksr
     6841 asiiretqhe salkkiqyle ddsrykrvdq edliiaekpk fgrplknieh lpegksahle
     6901 atltpvndpt mvvewfrdgr pipqghkfkt tydfgyvald ilyaypedsg tymckarnav
     6961 geavttcvis vdskqglfld tldaqrlqki reletvevkq evekevvhqk pvfltplnnl
     7021 dhlkegehah lecrvepind pnlkiewfvn gvavktghrf rkthdfgyva ldilytyped
     7081 sgtymckatn lageavntct ikvgsrrsil ldtqhpdgle kireleaqgh parleveepp
     7141 vtpprfvtel rgttevyegq tahfecqiep lhdanlrief fhngkplpsa srfhvtfdfg
     7201 yvaldightv pedageysvr ainalgqcvs sielrviprd niildsqrpe gldkirelea
     7261 qqpwkrpdip epqtrqrpvf tqplqnidai pegqtahfec rlipvgdptl kvewfrneip
     7321 letssritkv hdfgyvsldi nhvreedegv ymcrasnplg eavttasmki kskasiqldt
     7381 qhpeaqrkia qleadkpsrp eepekvfdkp iftqlltgpt elwegqmary ecrvvpvgda
     7441 slrfewying velkmgsrfh vshdfgyvtl dilkviteds gvytckaink ageavssisl
     7501 kvkarsaida dsiqpdawqk iqlkeaemnk vpemfvdttp qqapvftkhl esydklvegq
     7561 hvyleaqvep radpnlrvew fkngislqtg trlrstfdfg lvtlsinglr pddsaiytck
     7621 atnllgeavs tcslkivdrh wllgdtlhpd alpkidaleq phvtsaeqpe piyeepvfit
     7681 hlnnvecveg dnvhfecnve pskdptmkie wfingkplpt garfkstydf gyvalditha
     7741 yeedsgvviv katnskgsaq tsgtlkctsk qsiylqtqhp qgeaglekvk eaedaylsky
     7801 rrpdegpehe ypkpiwtvpl qpefklgefe plhlegqvep kddpnlkiew yfngkvleha
     7861 srfkmtsdfg fvtldltdvy erdsgiytck aynkageaft sttiycstke niiersqhpk
     7921 gkegleaiqd leeslkrqeg appeseeghp pvftsqfenl tnlsegeiah feasltptgd
     7981 qtmvvewfyn gkvleashri rtvyafgmvv levlgtkied sgtyscratn kwgqaeisve
     8041 lqcvdkskgq kpkftthiqs leglkdgqsa hfectlipvg dpqmkvewfh ngqplrhssr
     8101 fkmvsdfgfv vmdiagvmah dtgeyvckas nkygedftka tlkcfgksgv yldslqpdsl
     8161 arirelesfg geqpttpttp vaeppkfitq ivditrlveg qsahfearlt pvndpdlkve
     8221 wyyngkklph ghryrtfhdf givildilyc yeensgvyec rafnkygedt tkatlkcfsk
     8281 aslilesqlp kgmeggleki qtledsmirt kdekvveerg kapmftvpls nidglreges
     8341 ahfearlipt ddpklkvewf wngkplrtgs rfrtfcdfgf vileispiyp edsgeyscra
     8401 tneygeavtt ctmkctgkrs iilesqlpkg mestidkiae leglgnepgq vtpeddtgkp
     8461 pefvttpsdl tltenslahf ecrltpinds smrvewfhng kpllagsrik tihdfgfvil
     8521 evancyqrds glytckatnr hgeatvnckl qvrgrqgiil epqlptnfkt gtesiqklee
     8581 alykrdeilt eeetpnppkf tvelkdieve egapshfdcr vepvgdstmr idwfhngrsf
     8641 atgsrvhqin dfgfisldms ytyardsgey vcratnkwgs attkatitck skktidfdsq
     8701 lpsgmsgekl kelergpvse ppveeaprqp prfithiqsa tvdesepvrf ecrvepkddp
     8761 nlriewyrng kmipaghryr tmydmgfvsm dilyvypeds geyvckaind lgedttrasl
     8821 sckklpsiil qnqvpkgmkk sealmqmeat ikkytsevhl teddlydadk kqpprfvtqi
     8881 ldqtelvemn sskfecqlap vgdpnmkvew ffngkplphk nrftpiydfg yvamnfgwvy
     8941 pedsgeylcr atnlygmdet ravirtagkp giiyesqlpk gmksiekire meaawqivpe
     9001 eegeeekvra pptfvskpep vtveegdcsr fccrvtghpr prvmwiingh tvvngsrykl
     9061 tydgmyhldi pktrqydhgk veviarssvg esrtettltv kprsddyrgv lknsprpwyd
     9121 ygltqyqter qntelekvfd erhhnvsqgi eiatehlgqk vfkepetdwq ksvkskknee
     9181 yynklmtlee eqvlketrlr esshqfaipg ekvvadsvak gmaqkyeetl eekkdektqe
     9241 tvqppprplk kphttevdid vermkvtgky ppgpsestvh grevhvakqk qtqkevkgdl
     9301 eitrkitate ttevehkakt qervvqgptk pakppvftkk iqpcrafeqe qarfevefdg
     9361 dplptvkwyr edfpiqnsad lqiytfstks vlivrqvfme dsgvfsviae nrggkakcsa
     9421 nlvveerrrq pgrggvipps flstiqntsv ttgqlarfda kvtgtkpldv ywlkngkkit
     9481 adiryktlee dntytlllle tvpedsgkye cvainkagea rcdaectvrg pqspaksakp
     9541 ttpgvekapa vleplkdqti regtsvafsc risgkpvptv qwkkgdkvik pskyfqmske
     9601 gdfctlrise afpedegvyk ciaknpagdv ttsanlrvla pdaadvlpkl tplkdqivse
     9661 gqpaqfktqv tpskpkptiq wyregalipq spdfqmiheg nnavlliatt yeedtgtftc
     9721 rattsagtve tsaklivkkr tgayyevpae tgpgartgdk pfvslgtgkd vilepipldk
     9781 geleiplkpg detlpwrkgr vhhrprladt tpwrktkpks rdtsldklqa tesqikpwte
     9841 etvtlkktpq qareipkekl eevelkpaki ekkeirrpsl etvdlkpftk ettketkiee
     9901 kltrreeidk eiyeeeedsr flktsrkvde tvqrkkdevk pwteekvtlk kskpekkelp
     9961 ketletvelk pskigkgevr kpslekvdlk pvpttvtekv sekvtektey leqedtslld
    10021 vsrteevkkk rkekveekpe kpeqakpwte ekitlkkskp vvkeieketl etvelkpski
    10081 gkaelrkpsl ekvdlkpvpt tvtekvteki tekteyleqe dtslldvsrt eevkkmrkek
    10141 veekpeqakp wteekitlkk akhlpkeiek etvetvelkp skitkseirk ssvekvdlkt
    10201 vtketkeitq ktdyreeedt tllevtkdtq vkktrresip vekvegpkpw teekvtlkka
    10261 kpekkevpke kpetvelkps rpqkadiekp sledvdlkpi pkkvtkekte kdkyieeedt
    10321 sllevtkdte tttkvekkvk rreevekkpe tkpwtaekit lkkakveqke irresieevt
    10381 lkptktekrq vtketlekvd lqkvtdktqf veeedttlmk vdkvekveee rvdkmatwrr
    10441 drkpkekapy heeedksild vkrdeestek rkevpvpwar gkkkptekap yheeedtsll
    10501 dvkkdeesie kqkevpvpwa rgkkkpkeek pelvpqqvqp eekpeeeetl ektqvpwlrd
    10561 kkvarkiper keepvediks vtlkpvrrer apeekekpee vtlkpvkrip reeeekeevq
    10621 lkpipkrkpe ekpqeeiilk pfqkpkpeve eqekpklkpf ekakpevpeq ekvqlkpfkr
    10681 ekpevpekek velkpfkrek peetlvpqkv pekpeeeqps eveevswrrk pkkkeaiipe
    10741 kpvekveevk vvedkvikkq eetiedfksf tlkptkrgkk leekeekeev tlkpvkrlpk
    10801 eeetkaeikl kpvekkveek eakplkpema ptvpeeekpt evsevpwkrg kkkpvekrve
    10861 evtteekkve eeekevsels wrkpqkekvk evkkeqeved ftavmlkptr kekkpeekee
    10921 keeitlkpiq kvqkeeekpe avtlkpvkve kpeavekeev peektelpwr rgkkpakkiv
    10981 pqeevkpeei gkpeervpev keevpkkpkv ekpeeeklev klkpvkrlek peeakpeevt
    11041 lkpvkrlpke eeekeevklk pikrekpeee kpevklkpfk rlekpeeekp eevtlkpvkr
    11101 lpkeeeekee vklkpidrkk peeekpevkl kpikrlekpe eekpeevalk pvkrlpkeee
    11161 ekeeiklkpi krekpeeekp evklkpfkrl ekpeeekpee itlkpvkrrp keeeekeevk
    11221 lkpikkekpe eekpeiklkp ikrlekpeee kpeevtlkpv krlpkeeeek eevklkpikk
    11281 ekpeeekpev klkpfkrlek peeekpeeat lkpvkrlpke eeekeevklk pikkekpeee
    11341 kpevklkpvr rlekpeeekp eevtlkpvkr lpkeeedkee vklkpikkek peeekpevkl
    11401 kpvkrlpkee eekeevklkp ikkekpeeek pevklkpvkr lekpeeekpe evtlkpvkrl
    11461 pkeeeekeev klkpirkekp eeekpeeikl kpvkrldkpe eakpeevtlk pvkrlpkeee
    11521 ereevklkpv kkekpeeekp eeiklkpvkr lekpeetkpe evslkpvpkv vpekdeekpe
    11581 eeapetsilp wrrgkktkkv vdfheevtvv pidqkkivse vhekettive qkveakevie
    11641 kkvvveevkp evveevtevs evswrkprkp kkpveekevv elkpvekhee ivekveekit
    11701 lkkvkkevpe veekkeiqlk pvpkkpkeee vpekitlkpv kkekpeekpk eeeipelkpv
    11761 klekpekvee teekeevmve itrkkpkkkp eevpvekeev veteiteite ekpeeisvlp
    11821 wrrpkkvtek kieveeikpe kvedkeveev tettevswrk qrkpkkpiee eekpekvtlk
    11881 pvkrekpeek pkeeipelkp iklekpekve eteekeevkv evtrrrpkkk peeiqreeie
    11941 vetkevvddv evekiekqei qiedrkrkrk qrtqvdisii dkdkkiaprf iqklqpviae
    12001 pestakftct vfgspvpeis wyrneqelha sekyimtvye ttasleitkv keedagmysc
    12061 rasnpagvat stvnlvifek eeegiaphfs tpikplmvee hkpallecvv tgspvpevkw
    12121 yrgeqelkpk kgreitfnpe tgeaklhile pteedetiyr vravnkfgra ecranllinn
    12181 vvkvskpevl rapkitrplp alvaergkpl tlsadfeskp tpevkwfrng velapseknv
    12241 iniyentael fipevtkkdg gkyeirvqnp vgearssgsv tvkdrpdktd evkaprfiep
    12301 lqpqivaege vvimetkves yptasfqwfh etrplestpq vrivtqenks ilmikeikse
    12361 yagtytcrae nvggsvtcta tinlletpwe etvelvsptf vkrlspvrvm dgesanltcv
    12421 vqgkptpkve wyhdskpike gkeitivqdt egvcslaite vfpedageyt cravnpvgea
    12481 vcttslivea yeyvpdseia ssivatsltt gqsgseedll spketplfdt dteesapeii
    12541 kklpqliptk dgeltrlevk vkgkpkpegk wykqgveivs sqefqieefe dgtsvltiae
    12601 typddtgeiv feahnplgvs ttmtylsveg ivgtkeyrkp ewvthmeemq ealratqtvp
    12661 rfiqeitdvy vkegdtavfe csysgnptpd vvwykndkml mstqnlkvri ydddkktvlt
    12721 ikhateeddg ayvckitset glatskaklh vtdvtdekkf yeeeeeveeq tievkpkekp
    12781 qkkklrkake vkekvkterv kvdkkevrke klvpkeqpee kkivdieetq ilestdiiee
    12841 kptevsqakk ivpiqepvlt eataslkkid dekpkdmvpk peerakrvie eregivvvse
    12901 vttedmapdf tveskvdraq itseslqtvt vsevhtetsv qevrraeakq rkakktvvte
    12961 eeifeerpke vvveekpkkk kktekvkvre evtieeiver qreniareve eimemlhake
    13021 fgpgeaplre latigylvrq gvsvneines lyrtenfpal ktpeaqnalv qlvereghgp
    13081 litqvlteet ttdesvvaat vgfrafmrmi elqhvtveev lthfapeafr prawevteat
    13141 eveteervte rvdvvrktev hfmerrderk avrtqdirdi keyedtrqth ttlrarkdrk
    13201 preetkedrd egvqvveiee iedteqekmk kkrkdveeie eeietvivet dskgrlaqkk
    13261 kkrddleeke dveeeeeiie kvrkelkpkr pkigrdevel eeieefdvte dksmpsimvn
    13321 vpsqrhqvvp ldrtteqapg kpelekaklt vdtitalteh ivpvqekeis disrikpier
    13381 kaslsispve pysttettvq astgefadtf kptsyeatpg iipsegvqvs etlvsdagvt
    13441 slsvskqeig rtaevsvtlq eattvyetmv rekevptedf vtpqtvkaed tilpqlslsv
    13501 yevheglted kleplktvat kprvnvtave plvveevrae dkpgkyypel ivptevatqs
    13561 visqrqrvte emhapekege yipgrlppsq vaqigisygn etavvqqnvv qesegvfise
    13621 kktetfeatt nvtvlegvsv stvqtqdtet sltieetkka vadlnviemt pavtvetvps
    13681 ekekeyqpgd kpdtktaets issleigsiq stvvqesegv yrpdekptka laetsirpee
    13741 hvmiseiqta dypsdfreel kyvqesgtvs vqlteakmiq etlthdreer meemvkpeer
    13801 mvdtsydvnr svevfqttsv ekeadlrvfe mpeshrgktv pthpvvslev emtqpednlg
    13861 evkkevpssg takvehvslq etivgetvva enlapvekdk apetkvaeia mneveslhtt
    13921 mvvvsekete ytqvsdvkga yastefttqv apmleevrsh spteeylpdq kptsgiaqps
    13981 hvplesitva vqelaekedl yktdvrpdqk tanvaltelt sisipevmqv itassaetlk
    14041 ketkmkeekg rveveelsst iisevisnet edvfttkatp kdqkadfnis greaaettqv
    14101 tamteaedla vkrpeeqkgk pqvdeltplt vshvetgeae kelptaevpk ektaqpnifg
    14161 rdvaetmqvl tvtsaeelaa pkapegqkgk pqveeltpls vsqvvsqeae salpapeapt
    14221 erkaqpdmtg rdiaetmevl tmtsadilae dvkpteqkgm pgleefvpls vsqtvsteke
    14281 dvlpspeapt tktaqpsltg rdiaetmqvv mmtsekelit alapethkgi pnveeltplt
    14341 vsqtvsheae dvfektaaps evtakpafsg revaqtsemm ivtnvgelek qvlpdeqkgq
    14401 aqldelmplt vsqvisgeve eglpeaekpk ektaqpgilg rdiaettqvl tvtsaeelaa
    14461 pkapegqkgk pqveelaplt vsqvvseeae gilpapeapt erkaqpdmtg rdiaqtmevl
    14521 tmtsvdtfte dkkpdeqkgh peleefvpls vtqtvsneme dvlpspeaps tkiaqpsltg
    14581 rdvaetmqil tmtseeelit aqtpeqhkgk pnveeltplt vsqtvsqeae eilekaaapt
    14641 evtakpafsg revaqtseim ivtnvgelek qvlpdeqkgq pqldeltplt isqiisgeve
    14701 eglpeaekpk ektaqpgilg rdiaettqvl tvtsaeelea pkapegqkgk pqveelaplt
    14761 vsqvvsqeae gilpvpeapt erkaqadltg rdvaemmevl tmasvdtlte dkkpaeqkgv
    14821 pgleefvpls vsqtvsteke evlpspeapt tkvaeasltg rdiaetmqvv tmtseeelis
    14881 arvpeqhkgi fnveeltslt vsqtishete eilekaaalg evtakpaftg revaqtsetl
    14941 ivtnvgelek qilpeeqkgk prvdemtsit vsqvvsgeve erlpeaekpk ektaqpsmlg
    15001 rdiaettqvl tmtsaeelaa pkapeeqkgk pqveelaplt vsqvvsqeae gvlpvpeapt
    15061 erqaqpdltg rdvaqitvvm smasadvmid dkkpeeqkgv pgveefvpls vsqtvsteke
    15121 dvlpspeapt tkmaqpsltg rdiaetmqvv tmtseeelit alapethkgk pnveeltslt
    15181 vsqtisqete vileksaaps eatakpaftg reiaqtseil avlnvgelek hvlpeeqkgk
    15241 pkleeltplt vsqiisgeae eglptaevpk ektaepsmlg rdiaettqvl tmasaeelma
    15301 pkapeeqkgk pqveelaplt isqvvsqeae gvlpvpeapt erkaqpdltg rdvaqttvvm
    15361 smanvdimie ekkpeeqrgi pgleefvpls vsqtvsteke dvlpspevpt tkmaqpsltg
    15421 hdvaetlqvl tmvseeqltt kapeeqkgkp nveeltsltv sqtvsheaed ilekavapse
    15481 atakpaflgr evaetsqvlt vttvgelqkd vlpeeqkgkp qleelmpltv sqivsgeaee
    15541 rlpeaekpke ktaepsmlgr diaettqvlt masaeellap kvpeeqkgkp qveelapltv
    15601 sqvvsqeaeg tlpipeapte rtaqpdltgr dvaetmevlt masldtlied kkpaaqtgap
    15661 gleefiplsv sqmvsteked vlpspevpte kmaqpsligr dvaetmqvlt misamelttg
    15721 hapdqqkgvp nveeltsltv sqtvshetee ilekavapst vtakaalsgr evaetsqvlt
    15781 vsnveefeks ilpegqkgkp rldeltsitv sqvlsnelee tlpspekpse kiaqpqmigr
    15841 evaeqtevlt vtnveelkkl ekpegqtgkp dveemsfitv sevfstemek empspevpke
    15901 rtalpqlssi evaettevlt misaeellks kapeeqkvqp kldeltpltv shvvseeaee
    15961 alptaeipke ktaqpsllgr dvaetsqvlt masaeellaa kvpeeqkgkp qieelapltv
    16021 sqvvsqeaee alpsaevpve reaqpsmtsr dvaqtmlvts msnvdimaes kipeeqkgvp
    16081 gleefvslsv sqtvsteked vlpspevpte kmaqpsligr dvaettqilt misatelttd
    16141 rapeqqkgvp nveeltsltv sqavshetee ilekleapgt vtakaalsgr evaetsqvlt
    16201 vsnveefeks vlpeeqkgkp rldeltsitv sqvvsnelee tlpspekpse kvaqpqmigr
    16261 evaektevlt vsnveemqkl ekpegqkgip dveemsfitv sevvsteaek elpapeapke
    16321 rkalpqlsgi evaeksevlt mtmteefakp kapeeqkgkp qleellslsv sevalgevek
    16381 glptpdkpte etakpsllgi qvaeksqvia ssitgelqes ikpdtkqiip eqipfesieq
    16441 sqtltqeres tfvvekttts ataevsfrvs eslevtqvta tekeskevik gvakeasaqa
    16501 dvierkvalk tevqpedmas efkvkkpegk takgvdeiqq gvivtehlta hefeselpes
    16561 vlpfaklarv siqadhlehi vetteapaqq hhititqhtt khdlpqqqsi esdtevieey
    16621 ttkfrrgste dataavktkk tivrkkpktt geedivvvie eelddikpki psipkkqfmp
    16681 ieevttvsdv eeivsaride aeeivpkeee kvevtitevq dtdkkkkvtk kqfvrrrgkt
    16741 rqvteeitve eegkapettv vtkpveiiee dieerpkiek eeyvveevpe ektiplkprk
    16801 kltptvqqve eqvtvteeit kegkprkttk kkvitrkgke qqvtevvtve eqgkapitsv
    16861 tegpveevve eiiqklpapk yakpseveev reqvtvteev trlgkpkktt kkkiikrkgk
    16921 eqqvtevvtv eeegkapttt vtegpveevv ieeikelpip efvkptevee vreqvtvtee
    16981 ltkmgkpkkt ikkkiikrkg keqqvtevvt veeqgkapvt tvkegpveev veetitplla
    17041 peyakpseve evreqvtvtq evtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk
    17101 ttvtegpvee iieeeikpef ikpreveevr eqvtvtqevt regkpkktik kkiikkkgke
    17161 qqvtevvtve eqgkapvttv tegpveevvd etvkalpape yakpseveev reqvtvtqev
    17221 tklgkpkktt kkkiikrkgk eqqvteivtv eeegkapktt vtegpveeii eeeikpefik
    17281 preveevreq vtvtqevtre gkpkktikkk iikkkgkeeq vtevvtveeq gkapvttvne
    17341 gpveevvdet vkalpapeya epseveevre qvtvtqevtk lgkpkkttkk kiikrkgkeq
    17401 qvteivtvee egkapkttvt egpveeiiee eikpefikpr eveevreqvt vtqevtregk
    17461 pkktikkkii kkkgkeeqvt evvtveeqgk apvttvnegp veevvdetvk alpapeyaep
    17521 seveevreqv tvtqevtklg kpkkttkkki ikrkgkeqqv teivtveeeg kapkttvteg
    17581 pveeiieeei kpefikprev eevreqvtvt qevtregkpk ktikkkiikk kgkeqqvtel
    17641 vtveeqgkap vttvtegpve evvdetvkal papeyakpse veevreqvtv tqevtklgkp
    17701 kkttkkkiik rkgkeqqvte ivtveeegka pkttvtegpv eeiieeeikp efikprevee
    17761 vreqvtvtqe vtregkpkkt ikkkiikkkg keqqvtevvt veeqgkapvt tvtegpveev
    17821 vdetvkalpa peyakpseve evreqvtvtq evtklgkpkk ttkkkiikrk gkeqqvteiv
    17881 tveeegkapk ttvmegpvee vvieeiqelp ipefvkptev eevreqvtvt eevtrlgkpk
    17941 kttkkkiikr kgkeqqvtei vtveeqgkap vttvtegpve evvdetiksl papeyakpse
    18001 veevreqvtv tqevtklgkp kkttkkkivk rkgkeqqvte ivtveeegka pkttvtegpv
    18061 eevveeevkp llapefvkps eveevheqvt vtqevtregk pmktikkkii kkkgkaqqvt
    18121 evvtveeegk vpvttvkegp veevvdetvk alpapkyakp seveevreqv tvtqeitklg
    18181 kpkkttkrkv ikrkgkeqqv tevvtveeeg kapettvteg pveevleeei kplpgpefvk
    18241 pseveevreq vtvteevtre gkpkktikkk iikkkgkeqq vteivtveeq gkapvtsvte
    18301 gpveevvdet ikqlpapkya kpseveevre qvtvtqemtk lgkpkkttkk kiikrkgkeq
    18361 qvteivtvee egkapkttmt egpveevvee eikplpalef vkpseveevr eqvtvteevt
    18421 regkpkktik kkiikkkgke qqitevvtve eqgkapvttv tkapveeivd etikelpale
    18481 fakpseveev reqvtvtkev tkegkpkkti krkvikkkgk eqqvteivtv eeegkapktt
    18541 itegpveevv eeeikplpal efvkpsevee vreqvtvtee vtregkpkkt ikkkiikkkg
    18601 keqqvtevvt veeqgkapvt tvqegpveev idetikslpa peyakpseve eireqvtvte
    18661 eltklgkpkk ttkkkitkrk gpeqqiteiv tveeegkapv ttvtegpvee vvkevlkplp
    18721 vpkvvkpsev eeireqvtvt eemtkegkpk kiikkkvikr kgreqtvqev vtveekgkap
    18781 vttvtvgpte eiveetvktl papeyakpse veevreqvtv tqevtkegkp kkttkkkvik
    18841 rkgkeqqvte vvtveeqgka pvttvtegpv eevveellkp liapeyvkps eveevreqvt
    18901 vteeltkegk pkkvikkkvi krrgreqqvk evvtveeqgk apvttvtegp teeiveetik
    18961 alpapedakp seveevreqv tvtqevtkeg kpkktikkkv tkrkgkeqqv tevvtveekg
    19021 kapvttvteg pveeiveetv kalpapelvk ptevtevpee ltvtevvtke gktkkivkkk
    19081 vpkpkkegie apeeapeevp veetkkiikl qrlkvskfes tkldiqetsl gkpqfaqikl
    19141 rktpvakrps kkdekfpril lrsriiaiew ppspknlkvd tfepielqng ilsrnvdeaa
    19201 alpkpkkkki kriekeiakl ekldqefeel kkelplekle epileekper kvkkkkkped
    19261 kakegvkpql mrisikeqkp ikpkiaepat plfaqiklkk apakpkkkpe eevskfpkil
    19321 lrsritahew ppsvkypais vlepnyiqng ilsrtmeeal klkkvkhkkm klpekeltel
    19381 ekldqefeel kkvplekvee itpyerkpkp ekpeeekpkk lvigkgkrrp aeeedletvq
    19441 lkkipekips apeeaekvpk kkdevdkpkk ekeekeypel kpfepydiea peveleerek
    19501 peypepekpk ekkvpgkkpk kerktpaqet eavpivpgvp kpkepeeeee ikrriperpp
    19561 pteepekikl kpwkkgekpe eeekkpedyi kfvpkdddiv etvevtttve etpdkkqrki
    19621 kkkktitkpk dekpkiieet tieeegfepv vtveefdvdk yeavpvesve kaapveeike
    19681 eivtseiite egtkktvrkk rvtkkregke rvteevtvee egkepitati elhedtisei
    19741 heqakhkrpt kpkadeklae dvtektveqq eiieevkevk ptkkvikkek pvkakdevte
    19801 evveeiiiee pekqeakpiv eeeetviite hdvrkapkkk kevvikeeee ifetietpth
    19861 kiirkkirpm kkgekevvve eiiqkpreet vemeeqqive vvetptkkiv kkkkevvkdd
    19921 gvpeelveei iiekpqkkka kptveeekkk vkvtkvdtkk apekpeevve eeeeiietie
    19981 tptkkiirkk pkdkkdkiie eviqktieea kameeqeive vietptkkvi kkktttieeg
    20041 kapeekveei viekpkevie eeeeiietie tptkrivrkk pkdkkdkive eviqktieea
    20101 ktmkeqeiie vietptktvi kkkkatikeg kapeeeveei viekpikeka kkkilpkegv
    20161 eitevitets veelieeelk peeakpseev eeekvkitev aikkvpkkkk pskaveaeek
    20221 eevveeikpe kpkeekakpt vtekegievt evisevapek lpeeakpkkk kateiketee
    20281 kvitkkvkvk eapkeavpge eeqpaeevde lvpekpkktk akkevtpqds ivttevvpit
    20341 keeelevekp eeekatvvee keekakveek kvtvkkapkk klkpteaeee qveevveeit
    20401 peepkkrkak kavipkeeiq ttevipgita eeipeeekpe eekavpkeee kveeeakvte
    20461 vsvkkkerkk kekpvtngev ieekpkvkei peekpkeevp qvpeekekpe eekpkevkpk
    20521 ekkpkeekpk kpieekpeev kpeeekpevk peeekpkeek pkkkkpaekk pkiseeerpe
    20581 dkveeipekk peevktreei ipkdtvqtte vipetvaeei ptkkpkerka vpveekeekv
    20641 eitevpveka pekkeekvpe ekpteaapek pkeakvmkkk kkkpakkdei eewvepeyer
    20701 pvlepmpeki vwepkkkkek kplpeslqkl vpqkierkem kptklkysep aetvqfasik
    20761 lkkvavpkkk eadetkfpki mlrsrmtfvg dyppelqipk ityleenpvq tgilsrntee
    20821 alkvlkrkvk kvklsekevt qlekldlefe elkkvpleki edksvyerqp kkpaekpeep
    20881 kkvpigkgkv peeekvepek vklkkvpekk peeveeplkk pkkpepeeev kpekkkkpkv
    20941 klehdelkpl dfdrpeleky epeeyekpee papepvpkpy ekepkkkpdq eveqiplkkg
    21001 kpkspepdee pevkfriptk ekpeeepeqi tlkpwkkekp eeekekeipk keeekvpkpp
    21061 keepedvtvq rkaktkkpke kpkeekeeev tvkkpeeekp eevaeevtlk kepekpveea
    21121 paevtikkpv veekeveeee titevilkkk pekpeevvee avvkkpkkkk pvkeeevvee
    21181 vtvtkvvtee ekeeekvvik kpkkksipei teevteeitl kkpeekapee vteeikkpkk
    21241 kkpvkeevae eitikkpvve ekkeeevpee vtlkpkkpee kpvieevtee itlkkpvpee
    21301 vteevaikkp kkkkpveeea adeitlkkpv vvekeeeeit eevtlqkkkk pekkpvpeei
    21361 seeitlkkpv pkeeekapee vteeiaikkp kkkkpvkeea adevtikkpv veekkeeetp
    21421 eevtlkprkp kekpvpeevk eevtlkkpvp eekekvpeev teeiaikkpk kkkpveeeaa
    21481 devtikkpvv vekeeeeite eitlgkkkkp ekkpvpeeit eeitlkkpvp keeekapeev
    21541 tekievkkpk kkkpveeeaa devtikkpvv vekeeeeapa eitlkrrpke kpveeiteev
    21601 teeitikkpt peekekapee vsekvevkkp kkkkpvveea adevtikkvv veekkeeeap
    21661 eevtlkprpk kksvteeeva evtltkpkpi eeekkpeese ltikkkpkkk pvieeeaaev
    21721 tikkpvpvep deapeeitir kkkkpekkpk vteeveetav tikkarapek pteeeeittd
    21781 vqikkkkpqk kveevaadel tikkieeveq peeeeevqef tfkrkppklp pkpieeiyed
    21841 vtlrklrpkr ksrpdikeit etenvtfrpr ttktkedveq efkislntye eedismsgkv
    21901 rlkpkkkplt yseetgeeti riiqevedds gpiieeiies deegkdeysi eeldtdemrl
    21961 plrrkkkkvp kpykvediee gdvklklkre rkysieeade slalklkskr rvstydeeea
    22021 tlsitreedi seeveieyvv rdgdtmfsic syvaetdeai nlvegervyv idhtnqdwwf
    22081 vkkhlteekg wvpaqyllne vhytlylqrk lhekidklpv fekpapgdks sapkfveklq
    22141 pihtpdgyti qfecqvegvp rpqitwfrqt aiikpspdfq myydddnvat liirevfped
    22201 agtftcvakn aagfasstte liveaplsdh gsdltgpsrk slsresslad ilegipptfs
    22261 rkpkakyvde gedvilecrl vavpepeitw yykdtqittk eniiiatesd mhmycsviki
    22321 tkvqkkqegk ytivarnreg eatieipmkv ktgkseppei leplqsyvvr egetvvlstq
    22381 ivgnpapkvt wfkngkplkg ltpkqdghvn tltliqpqvs dtgeysvvat ndlgtaetka
    22441 tltveinlkp slainfqqht fehfsvtkei psgapepplf terfqeltvp ekgtfklvak
    22501 vtgnpvpevt wlrnnkplek spnitetydg enivleirna nsevdtgdyk cvasnpvgka
    22561 shgarvtvdv dkvtftktle kevvvdeykt leltcetsht vstkwwhndk eisgmdhrei
    22621 ieegrvhklv ikktnptdeg tykctvknqs tssnvtvkat kpefvkklqd cevkerdvai
    22681 leveitsqtv dvkwykdgeq lgpgkeklef vkegtvrkll irstsvhdeg eytcslldqe
    22741 ctaevtviel ppeiitkmqd vtiargerat fdieltkgda lvrwfkdgqe lqfsehvrls
    22801 idgkrqklki ydtesedagv yscevgqqks sakltveepa vefitklpdv tlvplnsdav
    22861 fvielsqdvp vtwmrktqvi kespkytiid egkvkklivk kcttedisey tamvanvkts
    22921 srlrveviet ppkispdtpr kykvrkdedv divvkfsgtp kpedewtvng rvvtkskrai
    22981 ptideesavl tirkiqeedv gdytlklvns vgetsieinv vivqvpsapg apeplettnd
    23041 svtlhwkkpd sdgnspivey ilehqektet twtkitetit etthkvtklt tnkeytfrvt
    23101 avneagpget sptspyvkis kpstaeppvi leplksivig lgetvtlscv iggtpapeit
    23161 wlrndeifed tsityenhit kytirettet ssakftvkak nnvgsaettc elkiqespki
    23221 iydenltnqk lpmngqwkie iqitgfpkpe vtwtkngkev dekrvsihre ertstvsiss
    23281 lvredtatym akaenqagss svdlhlkvid kpskpqgpvv fkeirqdrvt lewrppaddg
    23341 gielekytie kcepgktwvk vvdidkeves fcvhklqqna eykfriiarn avgaseples
    23401 dtvkmrtsfe ppgaprgple vsgmtktsft ikwqppendg gtpitdyiie ikeeskktwq
    23461 kigstkhett hfsvsdlktd vpynfritak nsvgsgppyv aeepiapgrr ikitkltess
    23521 myallgifpp tkivisktpp sspqhvhvtn itsksatlsw sppastggte ltgyviekrp
    23581 ligkgarwtk vvtldattlq ycvenlkese flfrifaenn qglsvpanse pvtlkthasv
    23641 pspptaplei rqiaantvvi swgrpdsdgg aplegykvai rdtkrtmwme varvnteiqk
    23701 lnirdlqenh mylirifarn eiglsdples eepfkiipas eltvvepiae atekgetasi
    23761 sfstentssw lrehnmdadi hsyararllr qdeyffriwh yakklfe
//
LOCUS       XP_034182853           23573 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034182853
VERSION     XP_034182853.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326962.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182853.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..23573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..23573
                     /product="sallimus isoform X9"
                     /calculated_mol_wt=2664230
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4272
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4490..4579
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4507..4511
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4520..4524
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4545..4549
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4559..4564
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4572..4575
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4736..4823
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4753..4757
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4766..4770
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4793..4797
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4807..4812
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4933..5022
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4950..4954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4963..4967
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4988..4992
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5002..5007
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5018
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5058..5148
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5075..5079
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5088..5092
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5114..5118
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5128..5133
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5141..5144
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5157..5247
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5174..5178
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5187..5191
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5213..5217
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5227..5232
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5240..5243
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5304..5395
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5321..5325
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5336..5340
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5362..5365
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5375..5380
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5388..5391
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5437..5529
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5455..5459
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5470..5474
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5495..5499
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5509..5514
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5522..5525
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5576..5668
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5594..5598
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5634..5638
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5648..5653
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5661..5664
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5710..5802
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5728..5732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5743..5747
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5769..5773
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5782..5787
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5795..5798
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5844..5936
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5862..5866
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5877..5881
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5902..5906
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5916..5921
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5929..5932
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5978..6069
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5995..5999
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6010..6014
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6036..6039
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6049..6054
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6062..6065
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6111..6201
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6129..6133
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6144..6148
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6169..6173
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6183..6188
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6196..6199
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6245..6336
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6262..6266
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6273..6281
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6302..6306
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6316..6321
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6329..6332
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6378..6469
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6395..6399
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6410..6414
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6435..6439
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6449..6454
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6462..6465
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6511..6603
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6529..6533
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6548
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6569..6573
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6583..6588
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6596..6599
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6645..6737
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6663..6667
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6676..6682
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6703..6707
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6717..6722
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6730..6733
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6777..6869
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6795..6799
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6808..6814
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6835..6839
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6849..6854
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6862..6865
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6910..7002
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6928..6932
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6941..6947
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6968..6972
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6982..6987
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6995..6998
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7044..7136
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7062..7066
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7077..7081
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7102..7106
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7116..7121
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7129..7132
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7176..7268
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7209..7213
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7234..7238
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7248..7253
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7310..7402
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7328..7332
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7343..7347
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7368..7372
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7382..7387
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7395..7398
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7442..7532
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7459..7463
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7474..7478
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7499..7503
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7513..7518
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7526..7529
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7609..7669
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7613..7617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7638..7642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7652..7657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7666..7669
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7717..7808
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7750..7754
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7775..7779
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7789..7794
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7818..7909
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7836..7840
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7851..7855
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7873..7880
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7895
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7903..7906
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7951..8042
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7969..7973
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7984..7988
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8006..8010
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8023..8028
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8036..8039
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8089..8180
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8122..8126
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8151
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8166
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8227..8318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8244..8248
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8259..8263
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8288
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8298..8303
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8311..8314
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8364..8454
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8381..8385
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8396..8400
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8421..8425
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8435..8440
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8448..8451
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8497..8586
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8514..8518
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8529..8533
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8554..8558
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8568..8573
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8581..8584
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8640..8730
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8658..8662
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8673..8677
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8695..8699
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8712..8717
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8725..8728
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8778..8866
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8795..8799
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8808..8812
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8832..8836
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8846..8851
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8862
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9101..9191
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9118..9122
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9131..9135
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9156..9160
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9170..9175
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9183..9186
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9205..9294
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9222..9226
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9235..9239
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9260..9264
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9274..9279
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9287..9290
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9315..9404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9332..9336
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9345..9349
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9370..9374
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9384..9389
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9397..9400
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9416..9503
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9430..9434
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9444..9448
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9469..9473
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9483..9488
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9496..9499
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <9620..10273
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10326..10479
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          11754..11843
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11771..11775
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11784..11788
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11809..11813
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11823..11828
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11853..11944
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11870..11874
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11883..11887
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11910..11914
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11924..11929
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11937..11940
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11959..12048
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11976..11980
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11989..11993
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12014..12018
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12028..12033
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12043..12046
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12061..12147
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12078..12082
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12091..12095
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12116..12120
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12130..12135
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12143..12146
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12164..12254
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12181..12185
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12194..12198
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12220..12224
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12234..12239
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12247..12250
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12302..12388
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12321..12325
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12334..12338
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12360..12364
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12374..12379
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12426..12517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12443..12447
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12456..12460
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12483..12487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12497..12502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12510..12513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16005..>16157
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          21811..21863
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(21816,21818,21824..21825,21842..21844,21857,21859,
                     21861..21862)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          21899..21989
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21916..21920
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21929..21933
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21955..21959
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21969..21974
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21982..21985
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22023..22116
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22040..22044
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22053..22057
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22078..22086
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22096..22101
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22109..22112
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22124..22210
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22141..22145
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22154..22158
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22176..22180
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22190..22195
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22203..22206
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22244..22334
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22261..22265
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22274..22278
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22299..22303
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22314..22319
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22327..22330
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22347..22424
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22357..22361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22368..22372
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22393..22397
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22407..22412
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22428..22513
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22445..22449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22457..22461
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22483..22487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22497..22502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22506..22509
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22518..22602
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22535..22539
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22547..22551
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22572..22576
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22586..22591
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22595..22598
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22708..22788
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22716..22720
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22729..22733
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22754..22758
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22768..22773
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22781..22784
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22792..22883
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22792,22857,22872)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(22873..22874,22876..22877)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          22894..22980
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22911..22915
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22924..22928
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22946..22950
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22960..22965
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22973..22976
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23004..23074
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          23004..23007
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          23016..23020
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          23040..23044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          23054..23059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          23067..23070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          23078..23170
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(23078,23144,23159)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <23148..23463
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(23160..23161,23163..23164)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..23573
                     /gene="sls"
                     /coded_by="XM_034326962.2:755..71476"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgspwpttng vasskeviqp yleilpkkap lfitplrdia
     4501 vvsgqtarfe civqaepqpn ilwskdgrii enstsyeihy rngvcrltiv rafpedagty
     4561 actatnslgs tvtsatllvp gvpspfygty rdqnlsersa esppkyvalr dlflpqesdv
     4621 ktassnrqqq hnsstfeyaw svnrsyaggr yrekenatee cveitsrnpi arrleysdfq
     4681 andsfrdvad fcfdreystc npsiasprnt vqgeivmqtt ptvpaftgrp gdpsppvfeq
     4741 ifknarfaqg gnaifegrvr gnpkptvtwt hkgapllesw kirmsyddkt gavtlqinqi
     4801 gpgdegeytc saknqygeai csvyiqpegf gpppqqqigg yrkeyaqtfq steqksqtgs
     4861 qsfqqrsyqq tidkrsyvng tstsiedfkv dtfeyrllre tefresitrr fvgesdvqis
     4921 tvvdrslgpv appqisqkpr nskliegsda vftakisgnp kprltwfkng qriresqrve
     4981 mshsnqqatl rirvalpeds ghytllsenp qgctvssayl aiessdqvdq ayqtpresvk
     5041 tpqvestges dsgkvlppnf vrtctdrdat egkmtrfdcr vtgrpypevt wyingqqvan
     5101 dlthkilvne sgnnslmitn vsradagvvt cvarnkaget sfqcnlnvie keqvvapkfv
     5161 erftttnvke gepvvfmara vgtpvpritw qkdgvpitpg pdvristdgs gastldipca
     5221 klsdaawyqc taqnvagsta trarlfvetp kgaapeprrl nlprptkvie pepapgpevi
     5281 ylrhverakp ylpppeedrv yppprfiipl rdvhqieggr ihfeariepv gdptmrvewy
     5341 vngraldass ratsifrfgf isldlisiil qdsgeyvcrv vsstgvaesr atlsvtprat
     5401 ieqasqhpds lqyiqqledy skyqrqesve eissqrpafi rplqdlgelq egrnahfeaq
     5461 ltpvsdptmk vewykdgrpi tassrittif nfgyvslnil hlraedagsy tvravnrlge
     5521 avssaslrvy artsvttdlg ipeqqryiea aeeleayqqa mhqkyvqeqp eptsppefks
     5581 pikdqnsire ggfahfearl epvgdsdlrv ewlkdgrpve assrittffn fgyvaltiky
     5641 vtihdvgiyt craynragea httaqlsvis kndviydsqh ptglqkiqtl edssrysrqv
     5701 qeetqvtqap rflgtlkgtn kivegqrahf earvepqsdl tmtiewyhng kaitaanriq
     5761 tyhdfgyvai dilqvrpeda gtytvvarna lgearlsatm vvetrssvdt ssmhrtsyek
     5821 tqrleeskfv epqyhieeis kskpifvqpl sdpkpvsegk nihlecrlep mgdptmrvew
     5881 fqngrpvtvg srfrtyydfg fvaldivhst ildsgeytvr atnhlgtaht sacvrvigks
     5941 divtetqheq sleqiqmled aaryrktqqe evtvmqapqf trplhnietv eltnvhlecr
     6001 lqpvgdatmk vewfvngrpv ktghrfrpsy efdyvaldil gvypedsgvy tcqarnqlge
     6061 avtscsvrvh akkdlllesq hpegleriqy ledasrykrh elvdevvkvk prfvtapknq
     6121 enlhegqhah fecklepvtd snlkvewykn grpvtighrf rpihdfgyva ldvidliaed
     6181 sgtytcravn lvgsdevscs lscrstaqvl tdtkneqgle qihyledrsk yqrredveet
     6241 ttqapiftts lnnveikegq rahfecrlip vsdatmkvew fhnnkpvkag srfietnsfg
     6301 fvaldimyay pedsgtytcr akniigeait satanvhskk siytetqnee tlqrlhhled
     6361 tsryqrkttt eeivtqapvf tmpikdlkva enqaahfear vipvgdsklk vewlrngvpi
     6421 aasnrvttmh dfgyvalnmk yvnpedsgty tcravndlge avtsatlfvq skaalqfesq
     6481 hesalsklqa ledtsryqrh eeeeivvkek psftvqlngp tnlvegqsah yecriepypd
     6541 pnmkvewfhn gkplstghry rttcdfgfas ldvltvyaed sgtytckatn klgsaqssik
     6601 ldvksrasii retqhesalk kiqyleddsr ykrvdqedli iaekpkfgrp lkniehlpeg
     6661 ksahleatlt pvndptmvve wfrdgrpipq ghkfkttydf gyvaldilya ypedsgtymc
     6721 karnavgeav ttcvisvdsk qglfldtlda qrlqkirele tvevkqevek evvhqkpvfl
     6781 tplnnldhlk egehahlecr vepindpnlk iewfvngvav ktghrfrkth dfgyvaldil
     6841 ytypedsgty mckatnlage avntctikvg srrsilldtq hpdglekire leaqghparl
     6901 eveeppvtpp rfvtelrgtt evyegqtahf ecqieplhda nlrieffhng kplpsasrfh
     6961 vtfdfgyval dightvpeda geysvraina lgqcvssiel rviprdniil dsqrpegldk
     7021 ireleaqqpw krpdipepqt rqrpvftqpl qnidaipegq tahfecrlip vgdptlkvew
     7081 frneiplets sritkvhdfg yvsldinhvr eedegvymcr asnplgeavt tasmkikska
     7141 siqldtqhpe aqrkiaqlea dkpsrpeepe kvfdkpiftq lltgptelwe gqmaryecrv
     7201 vpvgdaslrf ewyingvelk mgsrfhvshd fgyvtldilk vitedsgvyt ckainkagea
     7261 vssislkvka rsaidadsiq pdawqkiqlk eaemnkvpem fvdttpqqap vftkhlesyd
     7321 klvegqhvyl eaqvepradp nlrvewfkng islqtgtrlr stfdfglvtl singlrpdds
     7381 aiytckatnl lgeavstcsl kivdrhwllg dtlhpdalpk idaleqphvt saeqpepiye
     7441 epvfithlnn vecvegdnvh fecnvepskd ptmkiewfin gkplptgarf kstydfgyva
     7501 ldithayeed sgvvivkatn skgsaqtsgt lkctskqsiy lqtqhpqgea glekvkeaed
     7561 aylskyrrpd egpeheypkp iwtvplqpef klgefeplhl egqvepkddp nlkiewyfng
     7621 kvlehasrfk mtsdfgfvtl dltdvyerds giytckaynk ageaftstti ycstkeniie
     7681 rsqhpkgkeg leaiqdlees lkrqegappe seeghppvft sqfenltnls egeiahfeas
     7741 ltptgdqtmv vewfyngkvl eashrirtvy afgmvvlevl gtkiedsgty scratnkwgq
     7801 aeisvelqcv dkskgqkpkf tthiqslegl kdgqsahfec tlipvgdpqm kvewfhngqp
     7861 lrhssrfkmv sdfgfvvmdi agvmahdtge yvckasnkyg edftkatlkc fgksgvylds
     7921 lqpdslarir elesfggeqp ttpttpvaep pkfitqivdi trlvegqsah fearltpvnd
     7981 pdlkvewyyn gkklphghry rtfhdfgivi ldilycyeen sgvyecrafn kygedttkat
     8041 lkcfskasli lesqlpkgme gglekiqtle dsmirtkdek vveergkapm ftvplsnidg
     8101 lregesahfe arliptddpk lkvewfwngk plrtgsrfrt fcdfgfvile ispiypedsg
     8161 eyscratney geavttctmk ctgkrsiile sqlpkgmest idkiaelegl gnepgqvtpe
     8221 ddtgkppefv ttpsdltlte nslahfecrl tpindssmrv ewfhngkpll agsriktihd
     8281 fgfvilevan cyqrdsglyt ckatnrhgea tvncklqvrg rqgiilepql ptnfktgtes
     8341 iqkleealyk rdeilteeet pnppkftvel kdieveegap shfdcrvepv gdstmridwf
     8401 hngrsfatgs rvhqindfgf isldmsytya rdsgeyvcra tnkwgsattk atitckskkt
     8461 idfdsqlpsg msgeklkele rgpvseppve eaprqpprfi thiqsatvde sepvrfecrv
     8521 epkddpnlri ewyrngkmip aghryrtmyd mgfvsmdily vypedsgeyv ckaindlged
     8581 ttraslsckk lpsiilqnqv pkgmkkseal mqmeatikky tsevhltedd lydadkkqpp
     8641 rfvtqildqt elvemnsskf ecqlapvgdp nmkvewffng kplphknrft piydfgyvam
     8701 nfgwvypeds geylcratnl ygmdetravi rtagkpgiiy esqlpkgmks iekiremeaa
     8761 wqivpeeege eekvrapptf vskpepvtve egdcsrfccr vtghprprvm wiinghtvvn
     8821 gsrykltydg myhldipktr qydhgkvevi arssvgesrt ettltvkprs ddyrgvlkns
     8881 prpwydyglt qyqterqnte lekvfderhh nvsqgieiat ehlgqkvfke petdwqksvk
     8941 skkneeyynk lmtleeeqvl ketrlressh qfaipgekvv adsvakgmaq kyeetleekk
     9001 dektqetvqp pprplkkpht tevdidverm kvtgkyppgp sestvhgrev hvakqkqtqk
     9061 evkgdleitr kitatettev ehkaktqerv vqgptkpakp pvftkkiqpc rafeqeqarf
     9121 evefdgdplp tvkwyredfp iqnsadlqiy tfstksvliv rqvfmedsgv fsviaenrgg
     9181 kakcsanlvv eerrrqpgrg gvippsflst iqntsvttgq larfdakvtg tkpldvywlk
     9241 ngkkitadir yktleednty tlllletvpe dsgkyecvai nkagearcda ectvrgpqsp
     9301 aksakpttpg vekapavlep lkdqtiregt svafscrisg kpvptvqwkk gdkvikpsky
     9361 fqmskegdfc tlriseafpe degvykciak npagdvttsa nlrvlapdaa dvlpkltplk
     9421 dqivsegqpa qfktqvtpsk pkptiqwyre galipqspdf qmihegnnav lliattyeed
     9481 tgtftcratt sagtvetsak livkkrtgay yevpaetgpg artgdkpfvs lgtgkdvile
     9541 pipldkgele iplkpgdetl pwrkgrvhhr prladttpwr ktkpksrdts ldklqatesq
     9601 ikpwteetvt lkktpqqare ipkekleeve lkpakiekke irrpsletvd lkpftkettk
     9661 etkieekltr reeidkeiye eeedsrflkt srkvdetvqr kkdevkpwte ekvtlkkskp
     9721 ekkelpketl etvelkpski gkgevrkpsl ekvdlkpvpt tvtekvsekv tekteyleqe
     9781 dtslldvsrt eevkkkrkek veekpekpeq akpwteekit lkkskpvvke ieketletve
     9841 lkpskigkae lrkpslekvd lkpvpttvte kvtekitekt eyleqedtsl ldvsrteevk
     9901 kmrkekveek peqakpwtee kitlkkakhl pkeieketve tvelkpskit kseirkssve
     9961 kvdlktvtke tkeitqktdy reeedttlle vtkdtqvkkt rresipvekv egpkpwteek
    10021 vtlkkakpek kevpkekpet velkpsrpqk adiekpsled vdlkpipkkv tkektekdky
    10081 ieeedtslle vtkdtetttk vekkvkrree vekkpetkpw taekitlkka kveqkeirre
    10141 sieevtlkpt ktekrqvtke tlekvdlqkv tdktqfveee dttlmkvdkv ekveeervdk
    10201 matwrrdrkp kekapyheee dksildvkrd eestekrkev pvpwargkkk ptekapyhee
    10261 edtslldvkk deesiekqke vpvpwargkk kpkeekpelv pqqvqpeekp eeeetlektq
    10321 vpwlrdkkva rkiperkeep vediksvtlk pvrrerapee kekpeevtlk pvkripreee
    10381 ekeevqlkpi pkrkpeekpq eeiilkpfqk pkpeveeqek pklkpfekak pevpeqekvq
    10441 lkpfkrekpe vpekekvelk pfkrekpeet lvpqkvpekp eeeqpsevee vswrrkpkkk
    10501 eaiipekpve kveevkvved kvikkqeeti edfksftlkp tkrgkkleek eekeevtlkp
    10561 vkrlpkeeet kaeiklkpve kkveekeakp lkpemaptvp eeekptevse vpwkrgkkkp
    10621 vekrveevtt eekkveeeek evselswrkp qkekvkevkk eqevedftav mlkptrkekk
    10681 peekeekeei tlkpiqkvqk eeekpeavtl kpvkvekpea vekeevpeek telpwrrgkk
    10741 pakkivpqee vkpeeigkpe ervpevkeev pkkpkvekpe eeklevklkp vkrlekpeea
    10801 kpeevtlkpv krlpkeeeek eevklkpikr ekpeeekpev klkpfkrlek peeekpeevt
    10861 lkpvkrlpke eeekeevklk pidrkkpeee kpevklkpik rlekpeeekp eevalkpvkr
    10921 lpkeeeekee iklkpikrek peeekpevkl kpfkrlekpe eekpeeitlk pvkrrpkeee
    10981 ekeevklkpi kkekpeeekp eiklkpikrl ekpeeekpee vtlkpvkrlp keeeekeevk
    11041 lkpikkekpe eekpevklkp fkrlekpeee kpeeatlkpv krlpkeeeek eevklkpikk
    11101 ekpeeekpev klkpvrrlek peeekpeevt lkpvkrlpke eedkeevklk pikkekpeee
    11161 kpevklkpvk rlpkeeeeke evklkpikke kpeeekpevk lkpvkrlekp eeekpeevtl
    11221 kpvkrlpkee eekeevklkp irkekpeeek peeiklkpvk rldkpeeakp eevtlkpvkr
    11281 lpkeeeeree vklkpvkkek peeekpeeik lkpvkrlekp eetkpeevsl kpvpkvvpek
    11341 deekpeeeap etsilpwrrg kktkkvvdfh eevtvvpidq kkivsevhek ettiveqkve
    11401 akeviekkvv veevkpevve evtevsevsw rkprkpkkpv eekevvelkp vekheeivek
    11461 veekitlkkv kkevpeveek keiqlkpvpk kpkeeevpek itlkpvkkek peekpkeeei
    11521 pelkpvklek pekveeteek eevmveitrk kpkkkpeevp vekeevvete iteiteekpe
    11581 eisvlpwrrp kkvtekkiev eeikpekved keveevtett evswrkqrkp kkpieeeekp
    11641 ekvtlkpvkr ekpeekpkee ipelkpikle kpekveetee keevkvevtr rrpkkkpeei
    11701 qreeievetk evvddvevek iekqeiqied rkrkrkqrtq vdisiidkdk kiaprfiqkl
    11761 qpviaepest akftctvfgs pvpeiswyrn eqelhaseky imtvyettas leitkvkeed
    11821 agmyscrasn pagvatstvn lvifekeeeg iaphfstpik plmveehkpa llecvvtgsp
    11881 vpevkwyrge qelkpkkgre itfnpetgea klhileptee detiyrvrav nkfgraecra
    11941 nllinnvvkv skpevlrapk itrplpalva ergkpltlsa dfeskptpev kwfrngvela
    12001 pseknviniy entaelfipe vtkkdggkye irvqnpvgea rssgsvtvkd rpdktdevka
    12061 prfieplqpq ivaegevvim etkvesypta sfqwfhetrp lestpqvriv tqenksilmi
    12121 keikseyagt ytcraenvgg svtctatinl letpweetve lvsptfvkrl spvrvmdges
    12181 anltcvvqgk ptpkvewyhd skpikegkei tivqdtegvc slaitevfpe dageytcrav
    12241 npvgeavctt sliveayeyv pdseiassiv atslttgqsg seedllspke tplfdtdtee
    12301 sapeiikklp qliptkdgel trlevkvkgk pkpegkwykq gveivssqef qieefedgts
    12361 vltiaetypd dtgeivfeah nplgvsttmt ylsvegivgt keyrkpewvt hmeemqealr
    12421 atqtvprfiq eitdvyvkeg dtavfecsys gnptpdvvwy kndkmlmstq nlkvriyddd
    12481 kktvltikha teeddgayvc kitsetglat skaklhvtdv tdekkfyeee eeveeqtiev
    12541 kpkekpqkkk lrkakevkek vktervkvdk kevrkeklvp keqpeekkiv dieetqiles
    12601 tdiieekpte vsqakkivpi qepvlteata slkkiddekp kdmvpkpeer akrvieereg
    12661 ivvvsevtte dmapdftves kvdraqitse slqtvtvsev htetsvqevr raeakqrkak
    12721 ktvvteeeif eerpkevvve ekpkkkkkte kvkvreevti eeiverqren iareveeime
    12781 mlhakefgpg eaplrelati gylvrqgvsv neineslyrt enfpalktpe aqnalvqlve
    12841 reghgplitq vlteetttde svvaatvgfr afmrmielqh vtveevlthf apeafrpraw
    12901 evteatevet eervtervdv vrktevhfme rrderkavrt qdirdikeye dtrqthttlr
    12961 arkdrkpree tkedrdegvq vveieeiedt eqekmkkkrk dveeieeeie tvivetdskg
    13021 rlaqkkkkrd dleekedvee eeeiiekvrk elkpkrpkig rdeveleeie efdvtedksm
    13081 psimvnvpsq rhqvvpldrt teqapgkpel ekakltvdti taltehivpv qekeisdisr
    13141 ikpierkasl sispvepyst tettvqastg efadtfkpts yeatpgiips egvqvsetlv
    13201 sdagvtslsv skqeigrtae vsvtlqeatt vyetmvreke vptedfvtpq tvkaedtilp
    13261 qlslsvyevh egltedklep lktvatkprv nvtaveplvv eevraedkpg kyypelivpt
    13321 evatqsvisq rqrvteemha pekegeyipg rlppsqvaqi gisygnetav vqqnvvqese
    13381 gvfisekkte tfeattnvtv legvsvstvq tqdtetslti eetkkavadl nviemtpavt
    13441 vetvpsekek eyqpgdkpdt ktaetsissl eigsiqstvv qesegvyrpd ekptkalaet
    13501 sirpeehvmi seiqtadyps dfreelkyvq esgtvsvqlt eakmiqetlt hdreermeem
    13561 vkpeermvdt sydvnrsvev fqttsvekea dlrvfempes hrgktvpthp vvslevemtq
    13621 pednlgevkk evpssgtakv ehvslqetiv getvvaenla pvekdkapet kvaeiamnev
    13681 eslhttmvvv seketeytqv sdvkgayast efttqvapml eevrshspte eylpdqkpts
    13741 giaqpshvpl esitvavqel aekedlyktd vrpdqktanv alteltsisi pevmqvitas
    13801 saetlkketk mkeekgrvev eelsstiise visnetedvf ttkatpkdqk adfnisgrea
    13861 aettqvtamt eaedlavkrp eeqkgkpqvd eltpltvshv etgeaekelp taevpkekta
    13921 qpnifgrdva etmqvltvts aeelaapkap egqkgkpqve eltplsvsqv vsqeaesalp
    13981 apeapterka qpdmtgrdia etmevltmts adilaedvkp teqkgmpgle efvplsvsqt
    14041 vstekedvlp speapttkta qpsltgrdia etmqvvmmts ekelitalap ethkgipnve
    14101 eltpltvsqt vsheaedvfe ktaapsevta kpafsgreva qtsemmivtn vgelekqvlp
    14161 deqkgqaqld elmpltvsqv isgeveeglp eaekpkekta qpgilgrdia ettqvltvts
    14221 aeelaapkap egqkgkpqve elapltvsqv vseeaegilp apeapterka qpdmtgrdia
    14281 qtmevltmts vdtftedkkp deqkghpele efvplsvtqt vsnemedvlp speapstkia
    14341 qpsltgrdva etmqiltmts eeelitaqtp eqhkgkpnve eltpltvsqt vsqeaeeile
    14401 kaaaptevta kpafsgreva qtseimivtn vgelekqvlp deqkgqpqld eltpltisqi
    14461 isgeveeglp eaekpkekta qpgilgrdia ettqvltvts aeeleapkap egqkgkpqve
    14521 elapltvsqv vsqeaegilp vpeapterka qadltgrdva emmevltmas vdtltedkkp
    14581 aeqkgvpgle efvplsvsqt vstekeevlp speapttkva easltgrdia etmqvvtmts
    14641 eeelisarvp eqhkgifnve eltsltvsqt isheteeile kaaalgevta kpaftgreva
    14701 qtsetlivtn vgelekqilp eeqkgkprvd emtsitvsqv vsgeveerlp eaekpkekta
    14761 qpsmlgrdia ettqvltmts aeelaapkap eeqkgkpqve elapltvsqv vsqeaegvlp
    14821 vpeapterqa qpdltgrdva qitvvmsmas advmiddkkp eeqkgvpgve efvplsvsqt
    14881 vstekedvlp speapttkma qpsltgrdia etmqvvtmts eeelitalap ethkgkpnve
    14941 eltsltvsqt isqetevile ksaapseata kpaftgreia qtseilavln vgelekhvlp
    15001 eeqkgkpkle eltpltvsqi isgeaeeglp taevpkekta epsmlgrdia ettqvltmas
    15061 aeelmapkap eeqkgkpqve elapltisqv vsqeaegvlp vpeapterka qpdltgrdva
    15121 qttvvmsman vdimieekkp eeqrgipgle efvplsvsqt vstekedvlp spevpttkma
    15181 qpsltghdva etlqvltmvs eeqlttkape eqkgkpnvee ltsltvsqtv sheaedilek
    15241 avapseatak paflgrevae tsqvltvttv gelqkdvlpe eqkgkpqlee lmpltvsqiv
    15301 sgeaeerlpe aekpkektae psmlgrdiae ttqvltmasa eellapkvpe eqkgkpqvee
    15361 lapltvsqvv sqeaegtlpi peaptertaq pdltgrdvae tmevltmasl dtliedkkpa
    15421 aqtgapglee fiplsvsqmv stekedvlps pevptekmaq psligrdvae tmqvltmisa
    15481 melttghapd qqkgvpnvee ltsltvsqtv sheteeilek avapstvtak aalsgrevae
    15541 tsqvltvsnv eefeksilpe gqkgkprlde ltsitvsqvl sneleetlps pekpsekiaq
    15601 pqmigrevae qtevltvtnv eelkklekpe gqtgkpdvee msfitvsevf stemekemps
    15661 pevpkertal pqlssievae ttevltmisa eellkskape eqkvqpklde ltpltvshvv
    15721 seeaeealpt aeipkektaq psllgrdvae tsqvltmasa eellaakvpe eqkgkpqiee
    15781 lapltvsqvv sqeaeealps aevpvereaq psmtsrdvaq tmlvtsmsnv dimaeskipe
    15841 eqkgvpglee fvslsvsqtv stekedvlps pevptekmaq psligrdvae ttqiltmisa
    15901 telttdrape qqkgvpnvee ltsltvsqav sheteeilek leapgtvtak aalsgrevae
    15961 tsqvltvsnv eefeksvlpe eqkgkprlde ltsitvsqvv sneleetlps pekpsekvaq
    16021 pqmigrevae ktevltvsnv eemqklekpe gqkgipdvee msfitvsevv steaekelpa
    16081 peapkerkal pqlsgievae ksevltmtmt eefakpkape eqkgkpqlee llslsvseva
    16141 lgevekglpt pdkpteetak psllgiqvae ksqviassit gelqesikpd tkqiipeqip
    16201 fesieqsqtl tqerestfvv ektttsatae vsfrvsesle vtqvtateke skevikgvak
    16261 easaqadvie rkvalktevq pedmasefkv kkpegktakg vdeiqqgviv tehltahefe
    16321 selpesvlpf aklarvsiqa dhlehivett eapaqqhhit itqhttkhdl pqqqsiesdt
    16381 evieeyttkf rrgstedata avktkktivr kkpkttgeed ivvvieeeld dikpkipsip
    16441 kkqfmpieev ttvsdveeiv sarideaeei vpkeeekvev titevqdtdk kkkvtkkqfv
    16501 rrrgktrqvt eeitveeegk apettvvtkp veiieediee rpkiekeeyv veevpeekti
    16561 plkprkkltp tvqqveeqvt vteeitkegk prkttkkkvi trkgkeqqvt evvtveeqgk
    16621 apitsvtegp veevveeiiq klpapkyakp seveevreqv tvteevtrlg kpkkttkkki
    16681 ikrkgkeqqv tevvtveeeg kaptttvteg pveevvieei kelpipefvk pteveevreq
    16741 vtvteeltkm gkpkktikkk iikrkgkeqq vtevvtveeq gkapvttvke gpveevveet
    16801 itpllapeya kpseveevre qvtvtqevtk lgkpkkttkk kiikrkgkeq qvteivtvee
    16861 egkapkttvt egpveeiiee eikpefikpr eveevreqvt vtqevtregk pkktikkkii
    16921 kkkgkeqqvt evvtveeqgk apvttvtegp veevvdetvk alpapeyakp seveevreqv
    16981 tvtqevtklg kpkkttkkki ikrkgkeqqv teivtveeeg kapkttvteg pveeiieeei
    17041 kpefikprev eevreqvtvt qevtregkpk ktikkkiikk kgkeeqvtev vtveeqgkap
    17101 vttvnegpve evvdetvkal papeyaepse veevreqvtv tqevtklgkp kkttkkkiik
    17161 rkgkeqqvte ivtveeegka pkttvtegpv eeiieeeikp efikprevee vreqvtvtqe
    17221 vtregkpkkt ikkkiikkkg keeqvtevvt veeqgkapvt tvnegpveev vdetvkalpa
    17281 peyaepseve evreqvtvtq evtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk
    17341 ttvtegpvee iieeeikpef ikpreveevr eqvtvtqevt regkpkktik kkiikkkgke
    17401 qqvtelvtve eqgkapvttv tegpveevvd etvkalpape yakpseveev reqvtvtqev
    17461 tklgkpkktt kkkiikrkgk eqqvteivtv eeegkapktt vtegpveeii eeeikpefik
    17521 preveevreq vtvtqevtre gkpkktikkk iikkkgkeqq vtevvtveeq gkapvttvte
    17581 gpveevvdet vkalpapeya kpseveevre qvtvtqevtk lgkpkkttkk kiikrkgkeq
    17641 qvteivtvee egkapkttvm egpveevvie eiqelpipef vkpteveevr eqvtvteevt
    17701 rlgkpkkttk kkiikrkgke qqvteivtve eqgkapvttv tegpveevvd etikslpape
    17761 yakpseveev reqvtvtqev tklgkpkktt kkkivkrkgk eqqvteivtv eeegkapktt
    17821 vtegpveevv eeevkpllap efvkpsevee vheqvtvtqe vtregkpmkt ikkkiikkkg
    17881 kaqqvtevvt veeegkvpvt tvkegpveev vdetvkalpa pkyakpseve evreqvtvtq
    17941 eitklgkpkk ttkrkvikrk gkeqqvtevv tveeegkape ttvtegpvee vleeeikplp
    18001 gpefvkpsev eevreqvtvt eevtregkpk ktikkkiikk kgkeqqvtei vtveeqgkap
    18061 vtsvtegpve evvdetikql papkyakpse veevreqvtv tqemtklgkp kkttkkkiik
    18121 rkgkeqqvte ivtveeegka pkttmtegpv eevveeeikp lpalefvkps eveevreqvt
    18181 vteevtregk pkktikkkii kkkgkeqqit evvtveeqgk apvttvtkap veeivdetik
    18241 elpalefakp seveevreqv tvtkevtkeg kpkktikrkv ikkkgkeqqv teivtveeeg
    18301 kapkttiteg pveevveeei kplpalefvk pseveevreq vtvteevtre gkpkktikkk
    18361 iikkkgkeqq vtevvtveeq gkapvttvqe gpveevidet ikslpapeya kpseveeire
    18421 qvtvteeltk lgkpkkttkk kitkrkgpeq qiteivtvee egkapvttvt egpveevvke
    18481 vlkplpvpkv vkpseveeir eqvtvteemt kegkpkkiik kkvikrkgre qtvqevvtve
    18541 ekgkapvttv tvgpteeive etvktlpape yakpseveev reqvtvtqev tkegkpkktt
    18601 kkkvikrkgk eqqvtevvtv eeqgkapvtt vtegpveevv eellkpliap eyvkpsevee
    18661 vreqvtvtee ltkegkpkkv ikkkvikrrg reqqvkevvt veeqgkapvt tvtegpteei
    18721 veetikalpa pedakpseve evreqvtvtq evtkegkpkk tikkkvtkrk gkeqqvtevv
    18781 tveekgkapv ttvtegpvee iveetvkalp apelvkptev tevpeeltvt evvtkegktk
    18841 kivkkkvpkp kkegieapee apeevpveet kkiiklqrlk vskfestkld iqetslgkpq
    18901 faqiklrktp vakrpskkde kfprillrsr iiaiewppsp knlkvdtfep ielqngilsr
    18961 nvdeaaalpk pkkkkikrie keiaklekld qefeelkkel plekleepil eekperkvkk
    19021 kkkpedkake gvkpqlmris ikeqkpikpk iaepatplfa qiklkkapak pkkkpeeevs
    19081 kfpkillrsr itahewppsv kypaisvlep nyiqngilsr tmeealklkk vkhkkmklpe
    19141 keltelekld qefeelkkvp lekveeitpy erkpkpekpe eekpkklvig kgkrrpaeee
    19201 dletvqlkki pekipsapee aekvpkkkde vdkpkkekee keypelkpfe pydieapeve
    19261 leerekpeyp epekpkekkv pgkkpkkerk tpaqeteavp ivpgvpkpke peeeeeikrr
    19321 iperppptee pekiklkpwk kgekpeeeek kpedyikfvp kdddivetve vtttveetpd
    19381 kkqrkikkkk titkpkdekp kiieettiee egfepvvtve efdvdkyeav pvesvekaap
    19441 veeikeeivt seiiteegtk ktvrkkrvtk kregkervte evtveeegke pitatielhe
    19501 dtiseiheqa khkrptkpka deklaedvte ktveqqeiie evkevkptkk vikkekpvka
    19561 kdevteevve eiiieepekq eakpiveeee tviitehdvr kapkkkkevv ikeeeeifet
    19621 ietpthkiir kkirpmkkge kevvveeiiq kpreetveme eqqivevvet ptkkivkkkk
    19681 evvkddgvpe elveeiiiek pqkkkakptv eeekkkvkvt kvdtkkapek peevveeeee
    19741 iietietptk kiirkkpkdk kdkiieeviq ktieeakame eqeiveviet ptkkvikkkt
    19801 ttieegkape ekveeiviek pkevieeeee iietietptk rivrkkpkdk kdkiveeviq
    19861 ktieeaktmk eqeiieviet ptktvikkkk atikegkape eeveeiviek pikekakkki
    19921 lpkegveite vitetsveel ieeelkpeea kpseeveeek vkitevaikk vpkkkkpska
    19981 veaeekeevv eeikpekpke ekakptvtek egievtevis evapeklpee akpkkkkate
    20041 iketeekvit kkvkvkeapk eavpgeeeqp aeevdelvpe kpkktkakke vtpqdsivtt
    20101 evvpitkeee levekpeeek atvveekeek akveekkvtv kkapkkklkp teaeeeqvee
    20161 vveeitpeep kkrkakkavi pkeeiqttev ipgitaeeip eeekpeeeka vpkeeekvee
    20221 eakvtevsvk kkerkkkekp vtngevieek pkvkeipeek pkeevpqvpe ekekpeeekp
    20281 kevkpkekkp keekpkkpie ekpeevkpee ekpevkpeee kpkeekpkkk kpaekkpkis
    20341 eeerpedkve eipekkpeev ktreeiipkd tvqttevipe tvaeeiptkk pkerkavpve
    20401 ekeekveite vpvekapekk eekvpeekpt eaapekpkea kvmkkkkkkp akkdeieewv
    20461 epeyerpvle pmpekivwep kkkkekkplp eslqklvpqk ierkemkptk lkysepaetv
    20521 qfasiklkkv avpkkkeade tkfpkimlrs rmtfvgdypp elqipkityl eenpvqtgil
    20581 srnteealkv lkrkvkkvkl sekevtqlek ldlefeelkk vplekiedks vyerqpkkpa
    20641 ekpeepkkvp igkgkvpeee kvepekvklk kvpekkpeev eeplkkpkkp epeeevkpek
    20701 kkkpkvkleh delkpldfdr pelekyepee yekpeepape pvpkpyekep kkkpdqeveq
    20761 iplkkgkpks pepdeepevk friptkekpe eepeqitlkp wkkekpeeek ekeipkkeee
    20821 kvpkppkeep edvtvqrkak tkkpkekpke ekeeevtvkk peeekpeeva eevtlkkepe
    20881 kpveeapaev tikkpvveek eveeeetite vilkkkpekp eevveeavvk kpkkkkpvke
    20941 eevveevtvt kvvteeekee ekvvikkpkk ksipeiteev teeitlkkpe ekapeevtee
    21001 ikkpkkkkpv keevaeeiti kkpvveekke eevpeevtlk pkkpeekpvi eevteeitlk
    21061 kpvpeevtee vaikkpkkkk pveeeaadei tlkkpvvvek eeeeiteevt lqkkkkpekk
    21121 pvpeeiseei tlkkpvpkee ekapeevtee iaikkpkkkk pvkeeaadev tikkpvveek
    21181 keeetpeevt lkprkpkekp vpeevkeevt lkkpvpeeke kvpeevteei aikkpkkkkp
    21241 veeeaadevt ikkpvvveke eeeiteeitl gkkkkpekkp vpeeiteeit lkkpvpkeee
    21301 kapeevteki evkkpkkkkp veeeaadevt ikkpvvveke eeeapaeitl krrpkekpve
    21361 eiteevteei tikkptpeek ekapeevsek vevkkpkkkk pvveeaadev tikkvvveek
    21421 keeeapeevt lkprpkkksv teeevaevtl tkpkpieeek kpeeseltik kkpkkkpvie
    21481 eeaaevtikk pvpvepdeap eeitirkkkk pekkpkvtee veetavtikk arapekptee
    21541 eeittdvqik kkkpqkkvee vaadeltikk ieeveqpeee eevqeftfkr kppklppkpi
    21601 eeiyedvtlr klrpkrksrp dikeiteten vtfrprttkt kedveqefki slntyeeedi
    21661 smsgkvrlkp kkkpltysee tgeetiriiq eveddsgpii eeiiesdeeg kdeysieeld
    21721 tdemrlplrr kkkkvpkpyk vedieegdvk lklkrerkys ieeadeslal klkskrrvst
    21781 ydeeeatlsi treediseev eieyvvrdgd tmfsicsyva etdeainlve gervyvidht
    21841 nqdwwfvkkh lteekgwvpa qyllnevhyt lylqrklhek idklpvfekp apgdkssapk
    21901 fveklqpiht pdgytiqfec qvegvprpqi twfrqtaiik pspdfqmyyd ddnvatliir
    21961 evfpedagtf tcvaknaagf assttelive aplsdhgsdl tgpsrkslsr essladileg
    22021 ipptfsrkpk akyvdegedv ilecrlvavp epeitwyykd tqittkenii iatesdmhmy
    22081 csvikitkvq kkqegkytiv arnregeati eipmkvktgk seppeilepl qsyvvreget
    22141 vvlstqivgn papkvtwfkn gkplkgltpk qdghvntltl iqpqvsdtge ysvvatndlg
    22201 taetkatltv einlkpslai nfqqhtfehf svtkeipsga pepplfterf qeltvpekgt
    22261 fklvakvtgn pvpevtwlrn nkplekspni tetydgeniv leirnansev dtgdykcvas
    22321 npvgkashga rvtvdvdkvt ftktlekevv vdeyktlelt cetshtvstk wwhndkeisg
    22381 mdhreiieeg rvhklvikkt nptdegtykc tvknqstssn vtvkatkpef vkklqdcevk
    22441 erdvaileve itsqtvdvkw ykdgeqlgpg keklefvkeg tvrkllirst svhdegeytc
    22501 slldqectae vtvielppei itkmqdvtia rgeratfdie ltkgdalvrw fkdgqelqfs
    22561 ehvrlsidgk rqklkiydte sedagvysce vgqqkssakl tveepavefi tklpdvtlvp
    22621 lnsdavfvie lsqdvpvtwm rktqvikesp kytiidegkv kklivkkctt ediseytamv
    22681 anvktssrlr vevietppki spdtprkykv rkdedvdivv kfsgtpkped ewtvngrvvt
    22741 kskraiptid eesavltirk iqeedvgdyt lklvnsvget sieinvvivq vpsapgapep
    22801 lettndsvtl hwkkpdsdgn spiveyileh qektettwtk itetitetth kvtklttnke
    22861 ytfrvtavne agpgetspts pyvkiskpst aeppvilepl ksiviglget vtlscviggt
    22921 papeitwlrn deifedtsit yenhitkyti rettetssak ftvkaknnvg saettcelki
    22981 qespkiiyde nltnqklpmn gqwkieiqit gfpkpevtwt kngkevdekr vsihreerts
    23041 tvsisslvre dtatymakae nqagsssvdl hlkvidkpsk pqgpvvfkei rqdrvtlewr
    23101 ppaddggiel ekytiekcep gktwvkvvdi dkevesfcvh klqqnaeykf riiarnavga
    23161 seplesdtvk mrtsfeppga prgplevsgm tktsftikwq ppendggtpi tdyiieikee
    23221 skktwqkigs tkhetthfsv sdlktdvpyn fritaknsvg sgppyvaeep iapgrrikit
    23281 kltessmyal lgifpptkiv isktppsspq hvhvtnitsk satlswsppa stggteltgy
    23341 viekrpligk garwtkvvtl dattlqycve nlkeseflfr ifaennqgls vpansepvtl
    23401 kthasvpspp tapleirqia antvviswgr pdsdggaple gykvairdtk rtmwmevarv
    23461 nteiqklnir dlqenhmyli rifarneigl sdpleseepf kiipaseltv vepiaeatek
    23521 getasisfst entsswlreh nmdadihsya rarllrqdey ffriwhyakk lfe
//
LOCUS       XP_034182863           23386 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_034182863
VERSION     XP_034182863.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326972.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182863.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..23386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..23386
                     /product="sallimus isoform X10"
                     /calculated_mol_wt=2641062
     Region          470..694
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            573..578
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <875..1529
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1122..1432
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1701..1790
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1718..1722
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1731..1735
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1756..1760
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1770..1775
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1783..1786
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1802..1891
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1819..1823
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1832..1836
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1857..1861
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1871..1876
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1884..1887
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1901..1990
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1918..1922
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1931..1935
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1956..1960
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1970..1975
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1983..1986
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2013..2102
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2030..2034
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2043..2047
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2068..2072
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2082..2087
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2095..2098
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2128..2218
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2145..2149
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2158..2162
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2184..2188
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2198..2203
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2211..2214
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2240..2328
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2257..2261
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2270..2274
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2294..2298
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2308..2313
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2321..2324
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2496..2585
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2513..2517
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2526..2530
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2548..2555
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2565..2570
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2578..2581
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2592..2682
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2609..2613
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2622..2626
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2648..2652
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2662..2667
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2675..2678
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3317..3588
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4303..4392
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4320..4324
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4333..4337
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4358..4362
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4372..4377
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4385..4388
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4549..4636
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4566..4570
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4579..4583
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4606..4610
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4620..4625
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4746..4835
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4763..4767
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4776..4780
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4801..4805
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4815..4820
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4828..4831
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4871..4961
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4888..4892
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4901..4905
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4927..4931
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4941..4946
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4954..4957
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4970..5060
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4987..4991
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5000..5004
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5026..5030
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5045
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5053..5056
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5117..5208
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5134..5138
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5149..5153
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5175..5178
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5188..5193
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5201..5204
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5250..5342
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5268..5272
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5283..5287
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5308..5312
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5322..5327
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5335..5338
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5389..5481
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5407..5411
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5422..5426
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5447..5451
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5461..5466
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5474..5477
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5523..5615
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5541..5545
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5556..5560
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5582..5586
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5595..5600
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5608..5611
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5657..5749
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5675..5679
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5690..5694
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5715..5719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5729..5734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5742..5745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5791..5882
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5808..5812
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5823..5827
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5849..5852
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5862..5867
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5875..5878
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5924..6014
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5942..5946
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5957..5961
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5982..5986
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5996..6001
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6009..6012
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6058..6149
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6075..6079
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6086..6094
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6115..6119
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6134
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6142..6145
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6191..6282
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6208..6212
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6223..6227
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6248..6252
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6262..6267
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6275..6278
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6324..6416
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6342..6346
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6357..6361
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6382..6386
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6396..6401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6409..6412
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6458..6550
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6476..6480
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6489..6495
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6516..6520
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6530..6535
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6543..6546
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6590..6682
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6608..6612
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6621..6627
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6648..6652
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6662..6667
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6675..6678
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6723..6815
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6741..6745
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6754..6760
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6781..6785
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6795..6800
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6808..6811
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6857..6949
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6875..6879
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6890..6894
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6915..6919
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6929..6934
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6942..6945
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6989..7081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7022..7026
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7047..7051
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7061..7066
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7123..7215
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7141..7145
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7156..7160
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7181..7185
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7195..7200
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7208..7211
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7255..7345
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7272..7276
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7287..7291
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7312..7316
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7326..7331
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7339..7342
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7422..7482
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7426..7430
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7451..7455
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7465..7470
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7479..7482
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7530..7621
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7563..7567
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7588..7592
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7602..7607
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7631..7722
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7649..7653
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7664..7668
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7686..7693
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7703..7708
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7716..7719
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7764..7855
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7782..7786
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7797..7801
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7819..7823
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7836..7841
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7849..7852
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7902..7993
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7935..7939
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7960..7964
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7974..7979
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8040..8131
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8057..8061
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8072..8076
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8097..8101
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8111..8116
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8124..8127
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8177..8267
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8194..8198
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8209..8213
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8234..8238
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8248..8253
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8261..8264
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8310..8399
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8327..8331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8342..8346
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8367..8371
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8381..8386
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8394..8397
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8453..8543
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8471..8475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8486..8490
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8508..8512
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8525..8530
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8538..8541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8591..8679
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8608..8612
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8621..8625
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8645..8649
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8659..8664
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8672..8675
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8914..9004
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8931..8935
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8944..8948
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8969..8973
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8983..8988
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8996..8999
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9018..9107
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9035..9039
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9048..9052
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9073..9077
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9087..9092
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9100..9103
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9128..9217
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9145..9149
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9158..9162
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9183..9187
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9197..9202
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9210..9213
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9229..9316
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9243..9247
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9257..9261
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9282..9286
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9296..9301
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9309..9312
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <9433..10086
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10139..10292
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          11567..11656
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11584..11588
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11597..11601
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11622..11626
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11636..11641
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11666..11757
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11683..11687
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11696..11700
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11723..11727
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11737..11742
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11750..11753
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11772..11861
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11789..11793
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11802..11806
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11827..11831
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11841..11846
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11856..11859
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11874..11960
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11891..11895
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11904..11908
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11929..11933
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11943..11948
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11956..11959
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11977..12067
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11994..11998
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12007..12011
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12033..12037
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12047..12052
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12060..12063
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12115..12201
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12134..12138
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12147..12151
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12173..12177
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12187..12192
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12239..12330
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12256..12260
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12269..12273
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12296..12300
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12310..12315
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12323..12326
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <15818..>15970
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          21624..21676
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(21629,21631,21637..21638,21655..21657,21670,21672,
                     21674..21675)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          21712..21802
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21729..21733
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21742..21746
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21768..21772
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21782..21787
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21795..21798
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21836..21929
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21853..21857
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21866..21870
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21891..21899
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21909..21914
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21922..21925
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21937..22023
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21954..21958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21967..21971
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21989..21993
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22003..22008
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22016..22019
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22057..22147
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22074..22078
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22087..22091
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22112..22116
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22127..22132
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22140..22143
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22160..22237
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          22170..22174
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22181..22185
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22206..22210
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22220..22225
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22241..22326
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22258..22262
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22270..22274
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22296..22300
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22310..22315
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22319..22322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22331..22415
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22348..22352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22360..22364
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22385..22389
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22399..22404
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22408..22411
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22521..22601
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22529..22533
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22542..22546
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22567..22571
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22581..22586
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22594..22597
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22605..22696
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22605,22670,22685)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(22686..22687,22689..22690)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          22707..22793
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22724..22728
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22737..22741
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22759..22763
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22773..22778
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22786..22789
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22817..22887
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22817..22820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22829..22833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22853..22857
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22867..22872
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22880..22883
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22891..22983
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22891,22957,22972)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <22961..23276
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(22973..22974,22976..22977)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..23386
                     /gene="sls"
                     /coded_by="XM_034326972.2:162..70322"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mseaedasgk gsnltaveai sarlaevtrl aekldarlce agartrpvpm sssamsstss
       61 lfsssnqptt tslaatsvqs qpisagissi tsmplictse stvpfgtkgv ctvlatkssv
      121 asskpverst spvqvnggis ggtgeavags epskpekkiv eedtremgse iyrdasedki
      181 ddsqeeklke eiedimeksc egsladgsni edyvtatecs itptarsrse sfvttpecee
      241 laatvaatsa sdicwnieep kfissnaavs sslikqeeas kgqkversna smesfkpdiy
      301 etngerqvek lvsmekrvde ksgkvvkevt etttlrvshd trlgiasykv isnttqdhre
      361 kvdvkeikdi erivspdvhe ktnglhhdte tgsktdsytr rdelstktdd tertikftki
      421 cevsqeekpr vvsgdtskdm tlflkeytrh hedsgiemsp tkkeetlecq dflenarksg
      481 ewlrlkvlev qpeltklgas veeatelssa hdevllrlqs kqspveellr qadqlisnqr
      541 praevyaama etlgqawrdv nellerrkqi ldcnvlfqcr aeecresmra lemacndtll
      601 pieieavknf lskihdlrkn mlealmgalq egknlldrlk eianegtlds rpdriklead
      661 havlkvekwl eelhdrrrli easfrsrktq leqclalall atdlrdleei lndriaalss
      721 scdqlgdsas saelllfelk klqaeakefq drsikitkst erlvssghfa geqateqaya
      781 ilgaaadyvn dldqyeslln ravaffdsar saltkldqle iqlvttehpq ystrlarfha
      841 qtvatiedvt akplaegyal ldvtgrgapg aegvkrtved lenrkirlme rctahekenl
      901 eisrmintfl ekhdelrkwl msipeaflqg hqdmgsdvpm aedfcrlhrq lsndlerrtd
      961 evehlefeil pirekleesq klelkskvee lqrswsktke lvanridlgs vylqfhmvad
     1021 eltrevesie selksnadvl derkveelgr rwkdlqplyv kltgsgkafl daaikiddpy
     1081 ldvpraclcv qtllekfanr qltvteswek wrtsieilre rrieherkie estrtlewvs
     1141 kfseqlypvi tsqsaettmi lkdlagskqr ilpelnkavs eldsrikgin tlaqkgevhv
     1201 deeilkrlhq mhedlrktar dyeillesli sifesieeve qkietlkiqv ervtslkkif
     1261 eveaafseid atkwsisdrl ghiklrieda ivrikqqepp gagvkdiekl rraldsltsd
     1321 feifwsqttt kveehrrtct fiedleridg elknlnehlk nvdarmgenl qtakatsasf
     1381 vqfektvtil eqriekfvqt teesikiltp qivddisllk erwrnlkkrm eetkkrmnlc
     1441 idyfvlleea kewnregskl lvviarkatm vkapkdatdl lheienylkp geevqerrie
     1501 klkelstivf gterlpqfne vivenrqmld sftvvsselr tlvqnlknae dlqeklriek
     1561 qeadeklqaa kvemaaaaaa reeaenarki aeklaaetle kaatevktlk eereaqtmsa
     1621 ppsfsvsaqt ekiektdesy vqetvtsavt tkeihilqkt eiektvpliq repspgkiiv
     1681 teeiheksek iekddvppla peftiplnda tvqegekfsf qcnlvghplp evvwykdgis
     1741 ilnnpdyltt yvrdvctlti eetfaedsak ytcrafniag saetsatltv ketapeeqps
     1801 ppvfvkelvp staiegsshq idcvvegnpl ptvqwfkndv nidnspdyvi tynngeavlk
     1861 feevlpedka iymckatnrl gqastsasln vqrtecttek pqfvtplsna mgragqrvkl
     1921 eceargkpmp tltwyhdgrs veetmnlkiq tdggrsslii seafakdagc ymvvakndag
     1981 eatvscnvsv kgrlphetsd sefacsdmep vvpkiqmplk dlrvqegrsv rldcvivgqp
     2041 epeviwyhdd qpvkesadfq llfqgdkctl liheafldda glykvvains ggeassqctl
     2101 tvtpvsvthe lektrtevee tvtagsppkf vklptdslia egenaifeca vvgepkpelk
     2161 wfsdsgeise seririqrke dgtsilrils aipedkgnyv vkafnahgea kafarlvvrs
     2221 lgdfrrkeef vqmeekliap tfkerfedrk vpdgvstkfe civvgkpspk iqwlfndrpv
     2281 hgkdflvsvs gdrqvltipe tgsthvgtis cvaenaagka tctarleigg sevqrdkkev
     2341 veekvyelve ripgdemhas sssdkhfsae kseiggenkv ltkmsetite sstthktlkk
     2401 eyisssmsss tgapgqepts vcvkstvhst eqsssadgap pvvqsykvee hekiiqdqpg
     2461 eirqertvvv sqdeegikrd ikatqipkpv rkstaprfvs pvtgmivdqg sdivlegiid
     2521 gfpqpvviws kngqelktkd smtityshnh vrlelknvnv kdagrytcta snevgsasst
     2581 adlvvkktif ppvfgrrlqa qlvkrgdrvn meveitgtpe ptvswfkddi pikerppelr
     2641 ikqqgncyml imekaakeha gkymvratna ggeaqsiadf avfeptpdtm vevhktviyd
     2701 vqdkklmqsd ekkaevpssk hltseqvstt kiqpaatikt tptamstsma qmsrtvekik
     2761 epdtelcrse kisstveshq merkseqkyh mklehkmpsi vepktegktv ikevireevr
     2821 ssepkstlie ekieneniet sriakkdals ffesmtrete tipkgpkemi klvddadghe
     2881 vkvgkltkny ekstsfqevk kpevkppell ttkkavheif tkleqgsips rgidnklfdf
     2941 pyepykptme tkktvveeti ssgssslqss mtmskmeskr eskmesksse llsegfnlvp
     3001 epppeigymp kpeevkkkrp evsikakqlq esfdktlspi dapiggvkif pspspkilet
     3061 pkvappsfsm ppppfqlekk eiveevcikk dvhekrdpqp lvrpispsrp wssssdvetr
     3121 sqmstdlsey rchsaasshq eilrsssprp sadglameks wakkcadstr kswppqedtp
     3181 ikykqewqmp gedyktsske fkreveetpe ggikktsmes sstterrsws skqetmdkms
     3241 krapsppkiq piiynaetik vdhtvnkiee kslyekyise cdvkksqkse kleeysskkw
     3301 tgaedlkapg lvrsletpkk ptpplyrpve piptdqiile pgpppeigyi papvvrekki
     3361 eqiektlevs lehkpakipp ggirtippap kkeeppalpp kdvritpppl pakqptpvep
     3421 lepfpfkpsp sptiktpkva vpaptpskfv kgsfgvesdy esdmdshmka kwrpyesdne
     3481 epryrrvqpp vpkqprprst epeplppskf evpsestglp rpvltkeqqe kmykktttfk
     3541 rqeketkqqk yqvvqpppve lkpgsppiyv qpatksptpk sppakkpesp kfkvktfqqe
     3601 sgymadtdep lqqkssvasl sksfgkqess ssmsshmesr tsysesrsey fesksydsrq
     3661 qqqkkesfap sapssvkqpp qqrssfveks ygssavtssp rfmsaketly ttdqshkkfe
     3721 apkkilppsp spsrfvksef resdyesdyd gripplwkpr gyesddqsfr tvkpnlagpg
     3781 kpkvqredts rlieeastra qkaktfekre ervatkpktv llpgsppeia yappqpqpty
     3841 yearsgipfh naigtetkkt vrmdestens rrivtveqts rvikfgdnqk satdfdsfds
     3901 ssaqkqkthy kvptpkkfvq gqfresdyes didtrikpkw appesdseep ryrkvqpptt
     3961 kttrsssapv rqehvvspme fdtgptvlrk qtsmsqlrde rkfdgqvkrq assrqeetlk
     4021 pgsppefgyi srsdvkkaan hvasrhmsdm tssfksktek fvtdiqsdlk qgkpilkqpv
     4081 dgrasdadep rtyreesrls ehgtkqidpd tgliyfkydf gyefgivlpg egkktvssts
     4141 rsvqgqrras dievpivhef ttrkengfak rsasssstar qgkpagkfsg sktvkwepts
     4201 eseysesedi rnarkrhseg gntmtapslv ipcspspsrw dhttpsplsl spslpslspr
     4261 yssatgppsn vdsagspwpt tngvasskev iqpyleilpk kaplfitplr diavvsgqta
     4321 rfecivqaep qpnilwskdg riienstsye ihyrngvcrl tivrafpeda gtyactatns
     4381 lgstvtsatl lvpgvpspfy gtyrdqnlse rsaesppkyv alrdlflpqe sdvktassnr
     4441 qqqhnsstfe yawsvnrsya ggryrekena teecveitsr npiarrleys dfqandsfrd
     4501 vadfcfdrey stcnpsiasp rntvqgeivm qttptvpaft grpgdpsppv feqifknarf
     4561 aqggnaifeg rvrgnpkptv twthkgapll eswkirmsyd dktgavtlqi nqigpgdege
     4621 ytcsaknqyg eaicsvyiqp egfgpppqqq iggyrkeyaq tfqsteqksq tgsqsfqqrs
     4681 yqqtidkrsy vngtstsied fkvdtfeyrl lretefresi trrfvgesdv qistvvdrsl
     4741 gpvappqisq kprnsklieg sdavftakis gnpkprltwf kngqriresq rvemshsnqq
     4801 atlrirvalp edsghytlls enpqgctvss aylaiessdq vdqayqtpre svktpqvest
     4861 gesdsgkvlp pnfvrtctdr dategkmtrf dcrvtgrpyp evtwyingqq vandlthkil
     4921 vnesgnnslm itnvsradag vvtcvarnka getsfqcnln viekeqvvap kfverftttn
     4981 vkegepvvfm aravgtpvpr itwqkdgvpi tpgpdvrist dgsgastldi pcaklsdaaw
     5041 yqctaqnvag statrarlfv etpkgaapep rrlnlprptk viepepapgp eviylrhver
     5101 akpylpppee drvyppprfi iplrdvhqie ggrihfeari epvgdptmrv ewyvngrald
     5161 assratsifr fgfisldlis iilqdsgeyv crvvsstgva esratlsvtp ratieqasqh
     5221 pdslqyiqql edyskyqrqe sveeissqrp afirplqdlg elqegrnahf eaqltpvsdp
     5281 tmkvewykdg rpitassrit tifnfgyvsl nilhlraeda gsytvravnr lgeavssasl
     5341 rvyartsvtt dlgipeqqry ieaaeeleay qqamhqkyvq eqpeptsppe fkspikdqns
     5401 ireggfahfe arlepvgdsd lrvewlkdgr pveassritt ffnfgyvalt ikyvtihdvg
     5461 iytcraynra geahttaqls viskndviyd sqhptglqki qtledssrys rqvqeetqvt
     5521 qaprflgtlk gtnkivegqr ahfearvepq sdltmtiewy hngkaitaan riqtyhdfgy
     5581 vaidilqvrp edagtytvva rnalgearls atmvvetrss vdtssmhrts yektqrlees
     5641 kfvepqyhie eiskskpifv qplsdpkpvs egknihlecr lepmgdptmr vewfqngrpv
     5701 tvgsrfrtyy dfgfvaldiv hstildsgey tvratnhlgt ahtsacvrvi gksdivtetq
     5761 heqsleqiqm ledaaryrkt qqeevtvmqa pqftrplhni etveltnvhl ecrlqpvgda
     5821 tmkvewfvng rpvktghrfr psyefdyval dilgvypeds gvytcqarnq lgeavtscsv
     5881 rvhakkdlll esqhpegler iqyledasry krhelvdevv kvkprfvtap knqenlhegq
     5941 hahfecklep vtdsnlkvew ykngrpvtig hrfrpihdfg yvaldvidli aedsgtytcr
     6001 avnlvgsdev scslscrsta qvltdtkneq gleqihyled rskyqrredv eetttqapif
     6061 ttslnnveik egqrahfecr lipvsdatmk vewfhnnkpv kagsrfietn sfgfvaldim
     6121 yaypedsgty tcrakniige aitsatanvh skksiytetq neetlqrlhh ledtsryqrk
     6181 ttteeivtqa pvftmpikdl kvaenqaahf earvipvgds klkvewlrng vpiaasnrvt
     6241 tmhdfgyval nmkyvnpeds gtytcravnd lgeavtsatl fvqskaalqf esqhesalsk
     6301 lqaledtsry qrheeeeivv kekpsftvql ngptnlvegq sahyecriep ypdpnmkvew
     6361 fhngkplstg hryrttcdfg fasldvltvy aedsgtytck atnklgsaqs sikldvksra
     6421 siiretqhes alkkiqyled dsrykrvdqe dliiaekpkf grplkniehl pegksahlea
     6481 tltpvndptm vvewfrdgrp ipqghkfktt ydfgyvaldi lyaypedsgt ymckarnavg
     6541 eavttcvisv dskqglfldt ldaqrlqkir eletvevkqe vekevvhqkp vfltplnnld
     6601 hlkegehahl ecrvepindp nlkiewfvng vavktghrfr kthdfgyval dilytypeds
     6661 gtymckatnl ageavntcti kvgsrrsill dtqhpdglek ireleaqghp arleveeppv
     6721 tpprfvtelr gttevyegqt ahfecqiepl hdanlrieff hngkplpsas rfhvtfdfgy
     6781 valdightvp edageysvra inalgqcvss ielrviprdn iildsqrpeg ldkireleaq
     6841 qpwkrpdipe pqtrqrpvft qplqnidaip egqtahfecr lipvgdptlk vewfrneipl
     6901 etssritkvh dfgyvsldin hvreedegvy mcrasnplge avttasmkik skasiqldtq
     6961 hpeaqrkiaq leadkpsrpe epekvfdkpi ftqlltgpte lwegqmarye crvvpvgdas
     7021 lrfewyingv elkmgsrfhv shdfgyvtld ilkvitedsg vytckainka geavssislk
     7081 vkarsaidad siqpdawqki qlkeaemnkv pemfvdttpq qapvftkhle sydklvegqh
     7141 vyleaqvepr adpnlrvewf kngislqtgt rlrstfdfgl vtlsinglrp ddsaiytcka
     7201 tnllgeavst cslkivdrhw llgdtlhpda lpkidaleqp hvtsaeqpep iyeepvfith
     7261 lnnvecvegd nvhfecnvep skdptmkiew fingkplptg arfkstydfg yvaldithay
     7321 eedsgvvivk atnskgsaqt sgtlkctskq siylqtqhpq geaglekvke aedaylskyr
     7381 rpdegpehey pkpiwtvplq pefklgefep lhlegqvepk ddpnlkiewy fngkvlehas
     7441 rfkmtsdfgf vtldltdvye rdsgiytcka ynkageafts ttiycstken iiersqhpkg
     7501 kegleaiqdl eeslkrqega ppeseeghpp vftsqfenlt nlsegeiahf easltptgdq
     7561 tmvvewfyng kvleashrir tvyafgmvvl evlgtkieds gtyscratnk wgqaeisvel
     7621 qcvdkskgqk pkftthiqsl eglkdgqsah fectlipvgd pqmkvewfhn gqplrhssrf
     7681 kmvsdfgfvv mdiagvmahd tgeyvckasn kygedftkat lkcfgksgvy ldslqpdsla
     7741 rirelesfgg eqpttpttpv aeppkfitqi vditrlvegq sahfearltp vndpdlkvew
     7801 yyngkklphg hryrtfhdfg ivildilycy eensgvyecr afnkygedtt katlkcfska
     7861 slilesqlpk gmegglekiq tledsmirtk dekvveergk apmftvplsn idglregesa
     7921 hfearliptd dpklkvewfw ngkplrtgsr frtfcdfgfv ileispiype dsgeyscrat
     7981 neygeavttc tmkctgkrsi ilesqlpkgm estidkiael eglgnepgqv tpeddtgkpp
     8041 efvttpsdlt ltenslahfe crltpindss mrvewfhngk pllagsrikt ihdfgfvile
     8101 vancyqrdsg lytckatnrh geatvncklq vrgrqgiile pqlptnfktg tesiqkleea
     8161 lykrdeilte eetpnppkft velkdievee gapshfdcrv epvgdstmri dwfhngrsfa
     8221 tgsrvhqind fgfisldmsy tyardsgeyv cratnkwgsa ttkatitcks kktidfdsql
     8281 psgmsgeklk elergpvsep pveeaprqpp rfithiqsat vdesepvrfe crvepkddpn
     8341 lriewyrngk mipaghryrt mydmgfvsmd ilyvypedsg eyvckaindl gedttrasls
     8401 ckklpsiilq nqvpkgmkks ealmqmeati kkytsevhlt eddlydadkk qpprfvtqil
     8461 dqtelvemns skfecqlapv gdpnmkvewf fngkplphkn rftpiydfgy vamnfgwvyp
     8521 edsgeylcra tnlygmdetr avirtagkpg iiyesqlpkg mksiekirem eaawqivpee
     8581 egeeekvrap ptfvskpepv tveegdcsrf ccrvtghprp rvmwiinght vvngsryklt
     8641 ydgmyhldip ktrqydhgkv eviarssvge srtettltvk prsddyrgvl knsprpwydy
     8701 gltqyqterq ntelekvfde rhhnvsqgie iatehlgqkv fkepetdwqk svkskkneey
     8761 ynklmtleee qvlketrlre sshqfaipge kvvadsvakg maqkyeetle ekkdektqet
     8821 vqppprplkk phttevdidv ermkvtgkyp pgpsestvhg revhvakqkq tqkevkgdle
     8881 itrkitatet tevehkaktq ervvqgptkp akppvftkki qpcrafeqeq arfevefdgd
     8941 plptvkwyre dfpiqnsadl qiytfstksv livrqvfmed sgvfsviaen rggkakcsan
     9001 lvveerrrqp grggvippsf lstiqntsvt tgqlarfdak vtgtkpldvy wlkngkkita
     9061 diryktleed ntytllllet vpedsgkyec vainkagear cdaectvrgp qspaksakpt
     9121 tpgvekapav leplkdqtir egtsvafscr isgkpvptvq wkkgdkvikp skyfqmskeg
     9181 dfctlrisea fpedegvykc iaknpagdvt tsanlrvlap daadvlpklt plkdqivseg
     9241 qpaqfktqvt pskpkptiqw yregalipqs pdfqmihegn navlliatty eedtgtftcr
     9301 attsagtvet saklivkkrt gayyevpaet gpgartgdkp fvslgtgkdv ilepipldkg
     9361 eleiplkpgd etlpwrkgrv hhrprladtt pwrktkpksr dtsldklqat esqikpwtee
     9421 tvtlkktpqq areipkekle evelkpakie kkeirrpsle tvdlkpftke ttketkieek
     9481 ltrreeidke iyeeeedsrf lktsrkvdet vqrkkdevkp wteekvtlkk skpekkelpk
     9541 etletvelkp skigkgevrk pslekvdlkp vpttvtekvs ekvtekteyl eqedtslldv
     9601 srteevkkkr kekveekpek peqakpwtee kitlkkskpv vkeieketle tvelkpskig
     9661 kaelrkpsle kvdlkpvptt vtekvtekit ekteyleqed tslldvsrte evkkmrkekv
     9721 eekpeqakpw teekitlkka khlpkeieke tvetvelkps kitkseirks svekvdlktv
     9781 tketkeitqk tdyreeedtt llevtkdtqv kktrresipv ekvegpkpwt eekvtlkkak
     9841 pekkevpkek petvelkpsr pqkadiekps ledvdlkpip kkvtkektek dkyieeedts
     9901 llevtkdtet ttkvekkvkr reevekkpet kpwtaekitl kkakveqkei rresieevtl
     9961 kptktekrqv tketlekvdl qkvtdktqfv eeedttlmkv dkvekveeer vdkmatwrrd
    10021 rkpkekapyh eeedksildv krdeestekr kevpvpwarg kkkptekapy heeedtslld
    10081 vkkdeesiek qkevpvpwar gkkkpkeekp elvpqqvqpe ekpeeeetle ktqvpwlrdk
    10141 kvarkiperk eepvediksv tlkpvrrera peekekpeev tlkpvkripr eeeekeevql
    10201 kpipkrkpee kpqeeiilkp fqkpkpevee qekpklkpfe kakpevpeqe kvqlkpfkre
    10261 kpevpekekv elkpfkrekp eetlvpqkvp ekpeeeqpse veevswrrkp kkkeaiipek
    10321 pvekveevkv vedkvikkqe etiedfksft lkptkrgkkl eekeekeevt lkpvkrlpke
    10381 eetkaeiklk pvekkveeke akplkpemap tvpeeekpte vsevpwkrgk kkpvekrvee
    10441 vtteekkvee eekevselsw rkpqkekvke vkkeqevedf tavmlkptrk ekkpeekeek
    10501 eeitlkpiqk vqkeeekpea vtlkpvkvek peavekeevp eektelpwrr gkkpakkivp
    10561 qeevkpeeig kpeervpevk eevpkkpkve kpeeeklevk lkpvkrlekp eeakpeevtl
    10621 kpvkrlpkee eekeevklkp ikrekpeeek pevklkpfkr lekpeeekpe evtlkpvkrl
    10681 pkeeeekeev klkpidrkkp eeekpevklk pikrlekpee ekpeevalkp vkrlpkeeee
    10741 keeiklkpik rekpeeekpe vklkpfkrle kpeeekpeei tlkpvkrrpk eeeekeevkl
    10801 kpikkekpee ekpeiklkpi krlekpeeek peevtlkpvk rlpkeeeeke evklkpikke
    10861 kpeeekpevk lkpfkrlekp eeekpeeatl kpvkrlpkee eekeevklkp ikkekpeeek
    10921 pevklkpvrr lekpeeekpe evtlkpvkrl pkeeedkeev klkpikkekp eeekpevklk
    10981 pvkrlpkeee ekeevklkpi kkekpeeekp evklkpvkrl ekpeeekpee vtlkpvkrlp
    11041 keeeekeevk lkpirkekpe eekpeeiklk pvkrldkpee akpeevtlkp vkrlpkeeee
    11101 reevklkpvk kekpeeekpe eiklkpvkrl ekpeetkpee vslkpvpkvv pekdeekpee
    11161 eapetsilpw rrgkktkkvv dfheevtvvp idqkkivsev hekettiveq kveakeviek
    11221 kvvveevkpe vveevtevse vswrkprkpk kpveekevve lkpvekheei vekveekitl
    11281 kkvkkevpev eekkeiqlkp vpkkpkeeev pekitlkpvk kekpeekpke eeipelkpvk
    11341 lekpekveet eekeevmvei trkkpkkkpe evpvekeevv eteiteitee kpeeisvlpw
    11401 rrpkkvtekk ieveeikpek vedkeveevt ettevswrkq rkpkkpieee ekpekvtlkp
    11461 vkrekpeekp keeipelkpi klekpekvee teekeevkve vtrrrpkkkp eeiqreeiev
    11521 etkevvddve vekiekqeiq iedrkrkrkq rtqvdisiid kdkkiaprfi qklqpviaep
    11581 estakftctv fgspvpeisw yrneqelhas ekyimtvyet tasleitkvk eedagmyscr
    11641 asnpagvats tvnlvifeke eegiaphfst pikplmveeh kpallecvvt gspvpevkwy
    11701 rgeqelkpkk greitfnpet geaklhilep teedetiyrv ravnkfgrae cranllinnv
    11761 vkvskpevlr apkitrplpa lvaergkplt lsadfeskpt pevkwfrngv elapseknvi
    11821 niyentaelf ipevtkkdgg kyeirvqnpv gearssgsvt vkdrpdktde vkaprfiepl
    11881 qpqivaegev vimetkvesy ptasfqwfhe trplestpqv rivtqenksi lmikeiksey
    11941 agtytcraen vggsvtctat inlletpwee tvelvsptfv krlspvrvmd gesanltcvv
    12001 qgkptpkvew yhdskpikeg keitivqdte gvcslaitev fpedageytc ravnpvgeav
    12061 cttsliveay eyvpdseias sivatslttg qsgseedlls pketplfdtd teesapeiik
    12121 klpqliptkd geltrlevkv kgkpkpegkw ykqgveivss qefqieefed gtsvltiaet
    12181 ypddtgeivf eahnplgvst tmtylsvegi vgtkeyrkpe wvthmeemqe alratqtvpr
    12241 fiqeitdvyv kegdtavfec sysgnptpdv vwykndkmlm stqnlkvriy dddkktvlti
    12301 khateeddga yvckitsetg latskaklhv tdvtdekkfy eeeeeveeqt ievkpkekpq
    12361 kkklrkakev kekvktervk vdkkevrkek lvpkeqpeek kivdieetqi lestdiieek
    12421 ptevsqakki vpiqepvlte ataslkkidd ekpkdmvpkp eerakrviee regivvvsev
    12481 ttedmapdft veskvdraqi tseslqtvtv sevhtetsvq evrraeakqr kakktvvtee
    12541 eifeerpkev vveekpkkkk ktekvkvree vtieeiverq reniarevee imemlhakef
    12601 gpgeaplrel atigylvrqg vsvneinesl yrtenfpalk tpeaqnalvq lvereghgpl
    12661 itqvlteett tdesvvaatv gfrafmrmie lqhvtveevl thfapeafrp rawevteate
    12721 veteervter vdvvrktevh fmerrderka vrtqdirdik eyedtrqtht tlrarkdrkp
    12781 reetkedrde gvqvveieei edteqekmkk krkdveeiee eietvivetd skgrlaqkkk
    12841 krddleeked veeeeeiiek vrkelkpkrp kigrdevele eieefdvted ksmpsimvnv
    12901 psqrhqvvpl drtteqapgk pelekakltv dtitaltehi vpvqekeisd isrikpierk
    12961 aslsispvep ysttettvqa stgefadtfk ptsyeatpgi ipsegvqvse tlvsdagvts
    13021 lsvskqeigr taevsvtlqe attvyetmvr ekevptedfv tpqtvkaedt ilpqlslsvy
    13081 evhegltedk leplktvatk prvnvtavep lvveevraed kpgkyypeli vptevatqsv
    13141 isqrqrvtee mhapekegey ipgrlppsqv aqigisygne tavvqqnvvq esegvfisek
    13201 ktetfeattn vtvlegvsvs tvqtqdtets ltieetkkav adlnviemtp avtvetvpse
    13261 kekeyqpgdk pdtktaetsi ssleigsiqs tvvqesegvy rpdekptkal aetsirpeeh
    13321 vmiseiqtad ypsdfreelk yvqesgtvsv qlteakmiqe tlthdreerm eemvkpeerm
    13381 vdtsydvnrs vevfqttsve keadlrvfem peshrgktvp thpvvsleve mtqpednlge
    13441 vkkevpssgt akvehvslqe tivgetvvae nlapvekdka petkvaeiam neveslhttm
    13501 vvvseketey tqvsdvkgay astefttqva pmleevrshs pteeylpdqk ptsgiaqpsh
    13561 vplesitvav qelaekedly ktdvrpdqkt anvaltelts isipevmqvi tassaetlkk
    13621 etkmkeekgr veveelssti isevisnete dvfttkatpk dqkadfnisg reaaettqvt
    13681 amteaedlav krpeeqkgkp qvdeltpltv shvetgeaek elptaevpke ktaqpnifgr
    13741 dvaetmqvlt vtsaeelaap kapegqkgkp qveeltplsv sqvvsqeaes alpapeapte
    13801 rkaqpdmtgr diaetmevlt mtsadilaed vkpteqkgmp gleefvplsv sqtvsteked
    13861 vlpspeaptt ktaqpsltgr diaetmqvvm mtsekelita lapethkgip nveeltpltv
    13921 sqtvsheaed vfektaapse vtakpafsgr evaqtsemmi vtnvgelekq vlpdeqkgqa
    13981 qldelmpltv sqvisgevee glpeaekpke ktaqpgilgr diaettqvlt vtsaeelaap
    14041 kapegqkgkp qveelapltv sqvvseeaeg ilpapeapte rkaqpdmtgr diaqtmevlt
    14101 mtsvdtfted kkpdeqkghp eleefvplsv tqtvsnemed vlpspeapst kiaqpsltgr
    14161 dvaetmqilt mtseeelita qtpeqhkgkp nveeltpltv sqtvsqeaee ilekaaapte
    14221 vtakpafsgr evaqtseimi vtnvgelekq vlpdeqkgqp qldeltplti sqiisgevee
    14281 glpeaekpke ktaqpgilgr diaettqvlt vtsaeeleap kapegqkgkp qveelapltv
    14341 sqvvsqeaeg ilpvpeapte rkaqadltgr dvaemmevlt masvdtlted kkpaeqkgvp
    14401 gleefvplsv sqtvstekee vlpspeaptt kvaeasltgr diaetmqvvt mtseeelisa
    14461 rvpeqhkgif nveeltsltv sqtishetee ilekaaalge vtakpaftgr evaqtsetli
    14521 vtnvgelekq ilpeeqkgkp rvdemtsitv sqvvsgevee rlpeaekpke ktaqpsmlgr
    14581 diaettqvlt mtsaeelaap kapeeqkgkp qveelapltv sqvvsqeaeg vlpvpeapte
    14641 rqaqpdltgr dvaqitvvms masadvmidd kkpeeqkgvp gveefvplsv sqtvsteked
    14701 vlpspeaptt kmaqpsltgr diaetmqvvt mtseeelita lapethkgkp nveeltsltv
    14761 sqtisqetev ileksaapse atakpaftgr eiaqtseila vlnvgelekh vlpeeqkgkp
    14821 kleeltpltv sqiisgeaee glptaevpke ktaepsmlgr diaettqvlt masaeelmap
    14881 kapeeqkgkp qveelaplti sqvvsqeaeg vlpvpeapte rkaqpdltgr dvaqttvvms
    14941 manvdimiee kkpeeqrgip gleefvplsv sqtvsteked vlpspevptt kmaqpsltgh
    15001 dvaetlqvlt mvseeqlttk apeeqkgkpn veeltsltvs qtvsheaedi lekavapsea
    15061 takpaflgre vaetsqvltv ttvgelqkdv lpeeqkgkpq leelmpltvs qivsgeaeer
    15121 lpeaekpkek taepsmlgrd iaettqvltm asaeellapk vpeeqkgkpq veelapltvs
    15181 qvvsqeaegt lpipeapter taqpdltgrd vaetmevltm asldtliedk kpaaqtgapg
    15241 leefiplsvs qmvstekedv lpspevptek maqpsligrd vaetmqvltm isamelttgh
    15301 apdqqkgvpn veeltsltvs qtvsheteei lekavapstv takaalsgre vaetsqvltv
    15361 snveefeksi lpegqkgkpr ldeltsitvs qvlsneleet lpspekpsek iaqpqmigre
    15421 vaeqtevltv tnveelkkle kpegqtgkpd veemsfitvs evfstemeke mpspevpker
    15481 talpqlssie vaettevltm isaeellksk apeeqkvqpk ldeltpltvs hvvseeaeea
    15541 lptaeipkek taqpsllgrd vaetsqvltm asaeellaak vpeeqkgkpq ieelapltvs
    15601 qvvsqeaeea lpsaevpver eaqpsmtsrd vaqtmlvtsm snvdimaesk ipeeqkgvpg
    15661 leefvslsvs qtvstekedv lpspevptek maqpsligrd vaettqiltm isatelttdr
    15721 apeqqkgvpn veeltsltvs qavsheteei lekleapgtv takaalsgre vaetsqvltv
    15781 snveefeksv lpeeqkgkpr ldeltsitvs qvvsneleet lpspekpsek vaqpqmigre
    15841 vaektevltv snveemqkle kpegqkgipd veemsfitvs evvsteaeke lpapeapker
    15901 kalpqlsgie vaeksevltm tmteefakpk apeeqkgkpq leellslsvs evalgevekg
    15961 lptpdkptee takpsllgiq vaeksqvias sitgelqesi kpdtkqiipe qipfesieqs
    16021 qtltqerest fvvektttsa taevsfrvse slevtqvtat ekeskevikg vakeasaqad
    16081 vierkvalkt evqpedmase fkvkkpegkt akgvdeiqqg vivtehltah efeselpesv
    16141 lpfaklarvs iqadhlehiv etteapaqqh hititqhttk hdlpqqqsie sdtevieeyt
    16201 tkfrrgsted ataavktkkt ivrkkpkttg eedivvviee elddikpkip sipkkqfmpi
    16261 eevttvsdve eivsaridea eeivpkeeek vevtitevqd tdkkkkvtkk qfvrrrgktr
    16321 qvteeitvee egkapettvv tkpveiieed ieerpkieke eyvveevpee ktiplkprkk
    16381 ltptvqqvee qvtvteeitk egkprkttkk kvitrkgkeq qvtevvtvee qgkapitsvt
    16441 egpveevvee iiqklpapky akpseveevr eqvtvteevt rlgkpkkttk kkiikrkgke
    16501 qqvtevvtve eegkaptttv tegpveevvi eeikelpipe fvkpteveev reqvtvteel
    16561 tkmgkpkkti kkkiikrkgk eqqvtevvtv eeqgkapvtt vkegpveevv eetitpllap
    16621 eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    16681 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkeq
    16741 qvtevvtvee qgkapvttvt egpveevvde tvkalpapey akpseveevr eqvtvtqevt
    16801 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    16861 reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv tevvtveeqg kapvttvneg
    16921 pveevvdetv kalpapeyae pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    16981 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    17041 kktikkkiik kkgkeeqvte vvtveeqgka pvttvnegpv eevvdetvka lpapeyaeps
    17101 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp
    17161 veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtelv
    17221 tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk
    17281 kttkkkiikr kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev
    17341 reqvtvtqev tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt vtegpveevv
    17401 detvkalpap eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt
    17461 veeegkapkt tvmegpveev vieeiqelpi pefvkpteve evreqvtvte evtrlgkpkk
    17521 ttkkkiikrk gkeqqvteiv tveeqgkapv ttvtegpvee vvdetikslp apeyakpsev
    17581 eevreqvtvt qevtklgkpk kttkkkivkr kgkeqqvtei vtveeegkap kttvtegpve
    17641 evveeevkpl lapefvkpse veevheqvtv tqevtregkp mktikkkiik kkgkaqqvte
    17701 vvtveeegkv pvttvkegpv eevvdetvka lpapkyakps eveevreqvt vtqeitklgk
    17761 pkkttkrkvi krkgkeqqvt evvtveeegk apettvtegp veevleeeik plpgpefvkp
    17821 seveevreqv tvteevtreg kpkktikkki ikkkgkeqqv teivtveeqg kapvtsvteg
    17881 pveevvdeti kqlpapkyak pseveevreq vtvtqemtkl gkpkkttkkk iikrkgkeqq
    17941 vteivtveee gkapkttmte gpveevveee ikplpalefv kpseveevre qvtvteevtr
    18001 egkpkktikk kiikkkgkeq qitevvtvee qgkapvttvt kapveeivde tikelpalef
    18061 akpseveevr eqvtvtkevt kegkpkktik rkvikkkgke qqvteivtve eegkapktti
    18121 tegpveevve eeikplpale fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk
    18181 eqqvtevvtv eeqgkapvtt vqegpveevi detikslpap eyakpsevee ireqvtvtee
    18241 ltklgkpkkt tkkkitkrkg peqqiteivt veeegkapvt tvtegpveev vkevlkplpv
    18301 pkvvkpseve eireqvtvte emtkegkpkk iikkkvikrk greqtvqevv tveekgkapv
    18361 ttvtvgptee iveetvktlp apeyakpsev eevreqvtvt qevtkegkpk kttkkkvikr
    18421 kgkeqqvtev vtveeqgkap vttvtegpve evveellkpl iapeyvkpse veevreqvtv
    18481 teeltkegkp kkvikkkvik rrgreqqvke vvtveeqgka pvttvtegpt eeiveetika
    18541 lpapedakps eveevreqvt vtqevtkegk pkktikkkvt krkgkeqqvt evvtveekgk
    18601 apvttvtegp veeiveetvk alpapelvkp tevtevpeel tvtevvtkeg ktkkivkkkv
    18661 pkpkkegiea peeapeevpv eetkkiiklq rlkvskfest kldiqetslg kpqfaqiklr
    18721 ktpvakrpsk kdekfprill rsriiaiewp pspknlkvdt fepielqngi lsrnvdeaaa
    18781 lpkpkkkkik riekeiakle kldqefeelk kelpleklee pileekperk vkkkkkpedk
    18841 akegvkpqlm risikeqkpi kpkiaepatp lfaqiklkka pakpkkkpee evskfpkill
    18901 rsritahewp psvkypaisv lepnyiqngi lsrtmeealk lkkvkhkkmk lpekeltele
    18961 kldqefeelk kvplekveei tpyerkpkpe kpeeekpkkl vigkgkrrpa eeedletvql
    19021 kkipekipsa peeaekvpkk kdevdkpkke keekeypelk pfepydieap eveleerekp
    19081 eypepekpke kkvpgkkpkk erktpaqete avpivpgvpk pkepeeeeei krriperppp
    19141 teepekiklk pwkkgekpee eekkpedyik fvpkdddive tvevtttvee tpdkkqrkik
    19201 kkktitkpkd ekpkiieett ieeegfepvv tveefdvdky eavpvesvek aapveeikee
    19261 ivtseiitee gtkktvrkkr vtkkregker vteevtveee gkepitatie lhedtiseih
    19321 eqakhkrptk pkadeklaed vtektveqqe iieevkevkp tkkvikkekp vkakdevtee
    19381 vveeiiieep ekqeakpive eeetviiteh dvrkapkkkk evvikeeeei fetietpthk
    19441 iirkkirpmk kgekevvvee iiqkpreetv emeeqqivev vetptkkivk kkkevvkddg
    19501 vpeelveeii iekpqkkkak ptveeekkkv kvtkvdtkka pekpeevvee eeeiietiet
    19561 ptkkiirkkp kdkkdkiiee viqktieeak ameeqeivev ietptkkvik kktttieegk
    19621 apeekveeiv iekpkeviee eeeiietiet ptkrivrkkp kdkkdkivee viqktieeak
    19681 tmkeqeiiev ietptktvik kkkatikegk apeeeveeiv iekpikekak kkilpkegve
    19741 itevitetsv eelieeelkp eeakpseeve eekvkiteva ikkvpkkkkp skaveaeeke
    19801 evveeikpek pkeekakptv tekegievte visevapekl peeakpkkkk ateiketeek
    19861 vitkkvkvke apkeavpgee eqpaeevdel vpekpkktka kkevtpqdsi vttevvpitk
    19921 eeelevekpe eekatvveek eekakveekk vtvkkapkkk lkpteaeeeq veevveeitp
    19981 eepkkrkakk avipkeeiqt tevipgitae eipeeekpee ekavpkeeek veeeakvtev
    20041 svkkkerkkk ekpvtngevi eekpkvkeip eekpkeevpq vpeekekpee ekpkevkpke
    20101 kkpkeekpkk pieekpeevk peeekpevkp eeekpkeekp kkkkpaekkp kiseeerped
    20161 kveeipekkp eevktreeii pkdtvqttev ipetvaeeip tkkpkerkav pveekeekve
    20221 itevpvekap ekkeekvpee kpteaapekp keakvmkkkk kkpakkdeie ewvepeyerp
    20281 vlepmpekiv wepkkkkekk plpeslqklv pqkierkemk ptklkysepa etvqfasikl
    20341 kkvavpkkke adetkfpkim lrsrmtfvgd yppelqipki tyleenpvqt gilsrnteea
    20401 lkvlkrkvkk vklsekevtq lekldlefee lkkvplekie dksvyerqpk kpaekpeepk
    20461 kvpigkgkvp eeekvepekv klkkvpekkp eeveeplkkp kkpepeeevk pekkkkpkvk
    20521 lehdelkpld fdrpelekye peeyekpeep apepvpkpye kepkkkpdqe veqiplkkgk
    20581 pkspepdeep evkfriptke kpeeepeqit lkpwkkekpe eekekeipkk eeekvpkppk
    20641 eepedvtvqr kaktkkpkek pkeekeeevt vkkpeeekpe evaeevtlkk epekpveeap
    20701 aevtikkpvv eekeveeeet itevilkkkp ekpeevveea vvkkpkkkkp vkeeevveev
    20761 tvtkvvteee keeekvvikk pkkksipeit eevteeitlk kpeekapeev teeikkpkkk
    20821 kpvkeevaee itikkpvvee kkeeevpeev tlkpkkpeek pvieevteei tlkkpvpeev
    20881 teevaikkpk kkkpveeeaa deitlkkpvv vekeeeeite evtlqkkkkp ekkpvpeeis
    20941 eeitlkkpvp keeekapeev teeiaikkpk kkkpvkeeaa devtikkpvv eekkeeetpe
    21001 evtlkprkpk ekpvpeevke evtlkkpvpe ekekvpeevt eeiaikkpkk kkpveeeaad
    21061 evtikkpvvv ekeeeeitee itlgkkkkpe kkpvpeeite eitlkkpvpk eeekapeevt
    21121 ekievkkpkk kkpveeeaad evtikkpvvv ekeeeeapae itlkrrpkek pveeiteevt
    21181 eeitikkptp eekekapeev sekvevkkpk kkkpvveeaa devtikkvvv eekkeeeape
    21241 evtlkprpkk ksvteeevae vtltkpkpie eekkpeesel tikkkpkkkp vieeeaaevt
    21301 ikkpvpvepd eapeeitirk kkkpekkpkv teeveetavt ikkarapekp teeeeittdv
    21361 qikkkkpqkk veevaadelt ikkieeveqp eeeeevqeft fkrkppklpp kpieeiyedv
    21421 tlrklrpkrk srpdikeite tenvtfrprt tktkedveqe fkislntyee edismsgkvr
    21481 lkpkkkplty seetgeetir iiqeveddsg piieeiiesd eegkdeysie eldtdemrlp
    21541 lrrkkkkvpk pykvedieeg dvklklkrer kysieeades lalklkskrr vstydeeeat
    21601 lsitreedis eeveieyvvr dgdtmfsics yvaetdeain lvegervyvi dhtnqdwwfv
    21661 kkhlteekgw vpaqyllnev hytlylqrkl hekidklpvf ekpapgdkss apkfveklqp
    21721 ihtpdgytiq fecqvegvpr pqitwfrqta iikpspdfqm yydddnvatl iirevfpeda
    21781 gtftcvakna agfassttel iveaplsdhg sdltgpsrks lsressladi legipptfsr
    21841 kpkakyvdeg edvilecrlv avpepeitwy ykdtqittke niiiatesdm hmycsvikit
    21901 kvqkkqegky tivarnrege atieipmkvk tgkseppeil eplqsyvvre getvvlstqi
    21961 vgnpapkvtw fkngkplkgl tpkqdghvnt ltliqpqvsd tgeysvvatn dlgtaetkat
    22021 ltveinlkps lainfqqhtf ehfsvtkeip sgapepplft erfqeltvpe kgtfklvakv
    22081 tgnpvpevtw lrnnkpleks pnitetydge nivleirnan sevdtgdykc vasnpvgkas
    22141 hgarvtvdvd kvtftktlek evvvdeyktl eltcetshtv stkwwhndke isgmdhreii
    22201 eegrvhklvi kktnptdegt ykctvknqst ssnvtvkatk pefvkklqdc evkerdvail
    22261 eveitsqtvd vkwykdgeql gpgkeklefv kegtvrklli rstsvhdege ytcslldqec
    22321 taevtvielp peiitkmqdv tiargeratf dieltkgdal vrwfkdgqel qfsehvrlsi
    22381 dgkrqklkiy dtesedagvy scevgqqkss akltveepav efitklpdvt lvplnsdavf
    22441 vielsqdvpv twmrktqvik espkytiide gkvkklivkk cttediseyt amvanvktss
    22501 rlrvevietp pkispdtprk ykvrkdedvd ivvkfsgtpk pedewtvngr vvtkskraip
    22561 tideesavlt irkiqeedvg dytlklvnsv getsieinvv ivqvpsapga peplettnds
    22621 vtlhwkkpds dgnspiveyi lehqektett wtkitetite tthkvtkltt nkeytfrvta
    22681 vneagpgets ptspyvkisk pstaeppvil eplksivigl getvtlscvi ggtpapeitw
    22741 lrndeifedt sityenhitk ytirettets sakftvkakn nvgsaettce lkiqespkii
    22801 ydenltnqkl pmngqwkiei qitgfpkpev twtkngkevd ekrvsihree rtstvsissl
    22861 vredtatyma kaenqagsss vdlhlkvidk pskpqgpvvf keirqdrvtl ewrppaddgg
    22921 ielekytiek cepgktwvkv vdidkevesf cvhklqqnae ykfriiarna vgaseplesd
    22981 tvkmrtsfep pgaprgplev sgmtktsfti kwqppendgg tpitdyiiei keeskktwqk
    23041 igstkhetth fsvsdlktdv pynfritakn svgsgppyva eepiapgrri kitkltessm
    23101 yallgifppt kivisktpps spqhvhvtni tsksatlsws ppastggtel tgyviekrpl
    23161 igkgarwtkv vtldattlqy cvenlkesef lfrifaennq glsvpansep vtlkthasvp
    23221 spptapleir qiaantvvis wgrpdsdgga plegykvair dtkrtmwmev arvnteiqkl
    23281 nirdlqenhm ylirifarne iglsdplese epfkiipase ltvvepiaea tekgetasis
    23341 fstentsswl rehnmdadih syararllrq deyffriwhy akklfe
//
LOCUS       XP_034182870           23070 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_034182870
VERSION     XP_034182870.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182870.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..23070
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..23070
                     /product="sallimus isoform X11"
                     /calculated_mol_wt=2607072
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3987..4076
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4004..4008
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4017..4021
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4042..4046
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4056..4061
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4069..4072
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4233..4320
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4250..4254
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4263..4267
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4290..4294
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4304..4309
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4430..4519
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4447..4451
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4460..4464
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4485..4489
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4499..4504
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4512..4515
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4555..4645
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4572..4576
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4585..4589
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4611..4615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4625..4630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4638..4641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4654..4744
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4671..4675
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4684..4688
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4710..4714
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4724..4729
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4737..4740
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4801..4892
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4818..4822
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4833..4837
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4859..4862
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4872..4877
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4885..4888
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4934..5026
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4952..4956
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4967..4971
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4992..4996
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5006..5011
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5019..5022
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5073..5165
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5091..5095
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5106..5110
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5131..5135
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5145..5150
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5158..5161
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5207..5299
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5225..5229
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5240..5244
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5266..5270
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5279..5284
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5292..5295
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5341..5433
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5359..5363
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5374..5378
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5399..5403
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5413..5418
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5426..5429
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5475..5566
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5492..5496
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5507..5511
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5533..5536
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5546..5551
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5559..5562
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5608..5698
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5626..5630
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5641..5645
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5666..5670
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5680..5685
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5693..5696
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5742..5833
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5759..5763
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5770..5778
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5803
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5813..5818
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5826..5829
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5875..5966
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5892..5896
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5907..5911
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..5936
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5946..5951
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5959..5962
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6008..6100
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6026..6030
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6041..6045
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6066..6070
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6080..6085
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6093..6096
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6142..6234
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6160..6164
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6173..6179
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6200..6204
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6214..6219
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6227..6230
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6274..6366
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6292..6296
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6305..6311
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6332..6336
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6346..6351
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6359..6362
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6407..6499
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6425..6429
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6438..6444
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6465..6469
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6479..6484
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6492..6495
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6541..6633
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6559..6563
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6574..6578
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6599..6603
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6613..6618
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6626..6629
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6673..6765
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6706..6710
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6731..6735
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6745..6750
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6807..6899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6825..6829
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6840..6844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6865..6869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6879..6884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6892..6895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6939..7029
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6956..6960
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6971..6975
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6996..7000
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7010..7015
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7023..7026
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7106..7166
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7110..7114
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7135..7139
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7149..7154
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7163..7166
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7214..7305
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7247..7251
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7276
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7286..7291
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7315..7406
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7333..7337
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7348..7352
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7370..7377
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7387..7392
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7400..7403
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7448..7539
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7466..7470
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7481..7485
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7503..7507
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7520..7525
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7533..7536
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7586..7677
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7619..7623
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7644..7648
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7658..7663
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7724..7815
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7741..7745
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7756..7760
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7781..7785
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7795..7800
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7808..7811
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7861..7951
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7878..7882
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7893..7897
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7918..7922
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7932..7937
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7945..7948
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..8083
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8011..8015
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8026..8030
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8051..8055
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8065..8070
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8078..8081
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8137..8227
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8155..8159
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8170..8174
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8192..8196
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8209..8214
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8222..8225
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8275..8363
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8292..8296
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8305..8309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8329..8333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8343..8348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8356..8359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8598..8688
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8615..8619
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8628..8632
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8653..8657
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8667..8672
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8680..8683
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8702..8791
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8719..8723
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8732..8736
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8757..8761
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8771..8776
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8784..8787
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8812..8901
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8829..8833
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8842..8846
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8867..8871
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8881..8886
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8894..8897
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8913..9000
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8927..8931
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8941..8945
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8966..8970
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8980..8985
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8993..8996
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <9117..9770
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <9823..9976
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          11251..11340
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11268..11272
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11281..11285
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11306..11310
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11320..11325
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11350..11441
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11367..11371
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11380..11384
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11407..11411
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11421..11426
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11434..11437
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11456..11545
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11473..11477
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11486..11490
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11511..11515
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11525..11530
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11540..11543
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11558..11644
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11575..11579
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11588..11592
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11613..11617
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11627..11632
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11640..11643
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11661..11751
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11678..11682
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11691..11695
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11717..11721
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11731..11736
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11744..11747
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11799..11885
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          11818..11822
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11831..11835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11857..11861
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11871..11876
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11923..12014
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11940..11944
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11953..11957
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11980..11984
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11994..11999
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12007..12010
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <15502..>15654
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          21308..21360
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(21313,21315,21321..21322,21339..21341,21354,21356,
                     21358..21359)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          21396..21486
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21413..21417
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21426..21430
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21452..21456
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21466..21471
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21479..21482
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21520..21613
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21537..21541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21550..21554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21575..21583
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21593..21598
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21606..21609
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21621..21707
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21638..21642
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21651..21655
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21673..21677
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21687..21692
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21700..21703
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21741..21831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21758..21762
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21771..21775
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21796..21800
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21811..21816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21824..21827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21844..21921
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          21854..21858
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21865..21869
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21890..21894
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21904..21909
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21925..22010
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21942..21946
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21954..21958
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21980..21984
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21994..21999
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22003..22006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22015..22099
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22032..22036
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22044..22048
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22069..22073
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22083..22088
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22092..22095
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22205..22285
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22213..22217
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22226..22230
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22251..22255
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22265..22270
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22278..22281
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22289..22380
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22289,22354,22369)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(22370..22371,22373..22374)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          22391..22477
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22408..22412
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22421..22425
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22443..22447
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22457..22462
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22470..22473
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22501..22571
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22501..22504
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22513..22517
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22537..22541
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22551..22556
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22564..22567
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22575..22667
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22575,22641,22656)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <22645..22960
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(22657..22658,22660..22661)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..23070
                     /gene="sls"
                     /coded_by="XM_034326979.2:756..69968"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq etlyttdqsh kkfeapkkil ppspspsrfv
     3421 ksefresdye sdydgrippl wkprgyesdd qsfrtvkpnl agpgkpkvqr edtsrlieea
     3481 straqkaktf ekreervatk pktvllpgsp peiayappqp qptyyearsg ipfhnaigte
     3541 tkktvrmdes tensrrivtv eqtsrvikfg dnqksatdfd sfdsssaqkq kthykvptpk
     3601 kfvqgqfres dyesdidtri kpkwappesd seepryrkvq ppttkttrss sapvrqehvv
     3661 spmefdtgpt vlrkqtsmsq lrderkfdgq vkrqassrqe etlkpgsppe fgyisrsdvk
     3721 kaanhvasrh msdmtssfks ktekfvtdiq sdlkqgkpil kqpvdgrasd adeprtyree
     3781 srlsehgtkq idpdtgliyf kydfgyefgi vlpgegkktv sstsrsvqgq rrasdievpi
     3841 vhefttrken gfakrsasss starqgkpag kfsgsktvkw eptseseyse sedirnarkr
     3901 hseggntmta pslvipcsps psrwdhttps plslspslps lspryssatg ppsnvdsags
     3961 pwpttngvas skeviqpyle ilpkkaplfi tplrdiavvs gqtarfeciv qaepqpnilw
     4021 skdgriiens tsyeihyrng vcrltivraf pedagtyact atnslgstvt satllvpgvp
     4081 spfygtyrdq nlsersaesp pkyvalrdlf lpqesdvkta ssnrqqqhns stfeyawsvn
     4141 rsyaggryre kenateecve itsrnpiarr leysdfqand sfrdvadfcf dreystcnps
     4201 iasprntvqg eivmqttptv paftgrpgdp sppvfeqifk narfaqggna ifegrvrgnp
     4261 kptvtwthkg aplleswkir msyddktgav tlqinqigpg degeytcsak nqygeaicsv
     4321 yiqpegfgpp pqqqiggyrk eyaqtfqste qksqtgsqsf qqrsyqqtid krsyvngtst
     4381 siedfkvdtf eyrllretef resitrrfvg esdvqistvv drslgpvapp qisqkprnsk
     4441 liegsdavft akisgnpkpr ltwfkngqri resqrvemsh snqqatlrir valpedsghy
     4501 tllsenpqgc tvssaylaie ssdqvdqayq tpresvktpq vestgesdsg kvlppnfvrt
     4561 ctdrdategk mtrfdcrvtg rpypevtwyi ngqqvandlt hkilvnesgn nslmitnvsr
     4621 adagvvtcva rnkagetsfq cnlnviekeq vvapkfverf tttnvkegep vvfmaravgt
     4681 pvpritwqkd gvpitpgpdv ristdgsgas tldipcakls daawyqctaq nvagstatra
     4741 rlfvetpkga apeprrlnlp rptkviepep apgpeviylr hverakpylp ppeedrvypp
     4801 prfiiplrdv hqieggrihf eariepvgdp tmrvewyvng raldassrat sifrfgfisl
     4861 dlisiilqds geyvcrvvss tgvaesratl svtpratieq asqhpdslqy iqqledysky
     4921 qrqesveeis sqrpafirpl qdlgelqegr nahfeaqltp vsdptmkvew ykdgrpitas
     4981 srittifnfg yvslnilhlr aedagsytvr avnrlgeavs saslrvyart svttdlgipe
     5041 qqryieaaee leayqqamhq kyvqeqpept sppefkspik dqnsireggf ahfearlepv
     5101 gdsdlrvewl kdgrpveass rittffnfgy valtikyvti hdvgiytcra ynrageahtt
     5161 aqlsvisknd viydsqhptg lqkiqtleds srysrqvqee tqvtqaprfl gtlkgtnkiv
     5221 egqrahfear vepqsdltmt iewyhngkai taanriqtyh dfgyvaidil qvrpedagty
     5281 tvvarnalge arlsatmvve trssvdtssm hrtsyektqr leeskfvepq yhieeisksk
     5341 pifvqplsdp kpvsegknih lecrlepmgd ptmrvewfqn grpvtvgsrf rtyydfgfva
     5401 ldivhstild sgeytvratn hlgtahtsac vrvigksdiv tetqheqsle qiqmledaar
     5461 yrktqqeevt vmqapqftrp lhnietvelt nvhlecrlqp vgdatmkvew fvngrpvktg
     5521 hrfrpsyefd yvaldilgvy pedsgvytcq arnqlgeavt scsvrvhakk dlllesqhpe
     5581 gleriqyled asrykrhelv devvkvkprf vtapknqenl hegqhahfec klepvtdsnl
     5641 kvewykngrp vtighrfrpi hdfgyvaldv idliaedsgt ytcravnlvg sdevscslsc
     5701 rstaqvltdt kneqgleqih yledrskyqr redveetttq apifttslnn veikegqrah
     5761 fecrlipvsd atmkvewfhn nkpvkagsrf ietnsfgfva ldimyayped sgtytcrakn
     5821 iigeaitsat anvhskksiy tetqneetlq rlhhledtsr yqrkttteei vtqapvftmp
     5881 ikdlkvaenq aahfearvip vgdsklkvew lrngvpiaas nrvttmhdfg yvalnmkyvn
     5941 pedsgtytcr avndlgeavt satlfvqska alqfesqhes alsklqaled tsryqrheee
     6001 eivvkekpsf tvqlngptnl vegqsahyec riepypdpnm kvewfhngkp lstghryrtt
     6061 cdfgfasldv ltvyaedsgt ytckatnklg saqssikldv ksrasiiret qhesalkkiq
     6121 yleddsrykr vdqedliiae kpkfgrplkn iehlpegksa hleatltpvn dptmvvewfr
     6181 dgrpipqghk fkttydfgyv aldilyaype dsgtymckar navgeavttc visvdskqgl
     6241 fldtldaqrl qkireletve vkqevekevv hqkpvfltpl nnldhlkege hahlecrvep
     6301 indpnlkiew fvngvavktg hrfrkthdfg yvaldilyty pedsgtymck atnlageavn
     6361 tctikvgsrr silldtqhpd glekirelea qghparleve eppvtpprfv telrgttevy
     6421 egqtahfecq ieplhdanlr ieffhngkpl psasrfhvtf dfgyvaldig htvpedagey
     6481 svrainalgq cvssielrvi prdniildsq rpegldkire leaqqpwkrp dipepqtrqr
     6541 pvftqplqni daipegqtah fecrlipvgd ptlkvewfrn eipletssri tkvhdfgyvs
     6601 ldinhvreed egvymcrasn plgeavttas mkikskasiq ldtqhpeaqr kiaqleadkp
     6661 srpeepekvf dkpiftqllt gptelwegqm aryecrvvpv gdaslrfewy ingvelkmgs
     6721 rfhvshdfgy vtldilkvit edsgvytcka inkageavss islkvkarsa idadsiqpda
     6781 wqkiqlkeae mnkvpemfvd ttpqqapvft khlesydklv egqhvyleaq vepradpnlr
     6841 vewfkngisl qtgtrlrstf dfglvtlsin glrpddsaiy tckatnllge avstcslkiv
     6901 drhwllgdtl hpdalpkida leqphvtsae qpepiyeepv fithlnnvec vegdnvhfec
     6961 nvepskdptm kiewfingkp lptgarfkst ydfgyvaldi thayeedsgv vivkatnskg
     7021 saqtsgtlkc tskqsiylqt qhpqgeagle kvkeaedayl skyrrpdegp eheypkpiwt
     7081 vplqpefklg efeplhlegq vepkddpnlk iewyfngkvl ehasrfkmts dfgfvtldlt
     7141 dvyerdsgiy tckaynkage aftsttiycs tkeniiersq hpkgkeglea iqdleeslkr
     7201 qegappesee ghppvftsqf enltnlsege iahfeasltp tgdqtmvvew fyngkvleas
     7261 hrirtvyafg mvvlevlgtk iedsgtyscr atnkwgqaei svelqcvdks kgqkpkftth
     7321 iqsleglkdg qsahfectli pvgdpqmkve wfhngqplrh ssrfkmvsdf gfvvmdiagv
     7381 mahdtgeyvc kasnkygedf tkatlkcfgk sgvyldslqp dslarirele sfggeqpttp
     7441 ttpvaeppkf itqivditrl vegqsahfea rltpvndpdl kvewyyngkk lphghryrtf
     7501 hdfgivildi lycyeensgv yecrafnkyg edttkatlkc fskasliles qlpkgmeggl
     7561 ekiqtledsm irtkdekvve ergkapmftv plsnidglre gesahfearl iptddpklkv
     7621 ewfwngkplr tgsrfrtfcd fgfvileisp iypedsgeys cratneygea vttctmkctg
     7681 krsiilesql pkgmestidk iaeleglgne pgqvtpeddt gkppefvttp sdltltensl
     7741 ahfecrltpi ndssmrvewf hngkpllags riktihdfgf vilevancyq rdsglytcka
     7801 tnrhgeatvn cklqvrgrqg iilepqlptn fktgtesiqk leealykrde ilteeetpnp
     7861 pkftvelkdi eveegapshf dcrvepvgds tmridwfhng rsfatgsrvh qindfgfisl
     7921 dmsytyards geyvcratnk wgsattkati tckskktidf dsqlpsgmsg eklkelergp
     7981 vseppveeap rqpprfithi qsatvdesep vrfecrvepk ddpnlriewy rngkmipagh
     8041 ryrtmydmgf vsmdilyvyp edsgeyvcka indlgedttr aslsckklps iilqnqvpkg
     8101 mkksealmqm eatikkytse vhlteddlyd adkkqpprfv tqildqtelv emnsskfecq
     8161 lapvgdpnmk vewffngkpl phknrftpiy dfgyvamnfg wvypedsgey lcratnlygm
     8221 detravirta gkpgiiyesq lpkgmksiek iremeaawqi vpeeegeeek vrapptfvsk
     8281 pepvtveegd csrfccrvtg hprprvmwii nghtvvngsr ykltydgmyh ldipktrqyd
     8341 hgkveviars svgesrtett ltvkprsddy rgvlknsprp wydygltqyq terqntelek
     8401 vfderhhnvs qgieiatehl gqkvfkepet dwqksvkskk neeyynklmt leeeqvlket
     8461 rlresshqfa ipgekvvads vakgmaqkye etleekkdek tqetvqpppr plkkphttev
     8521 didvermkvt gkyppgpses tvhgrevhva kqkqtqkevk gdleitrkit atettevehk
     8581 aktqervvqg ptkpakppvf tkkiqpcraf eqeqarfeve fdgdplptvk wyredfpiqn
     8641 sadlqiytfs tksvlivrqv fmedsgvfsv iaenrggkak csanlvveer rrqpgrggvi
     8701 ppsflstiqn tsvttgqlar fdakvtgtkp ldvywlkngk kitadirykt leedntytll
     8761 lletvpedsg kyecvainka gearcdaect vrgpqspaks akpttpgvek apavleplkd
     8821 qtiregtsva fscrisgkpv ptvqwkkgdk vikpskyfqm skegdfctlr iseafpedeg
     8881 vykciaknpa gdvttsanlr vlapdaadvl pkltplkdqi vsegqpaqfk tqvtpskpkp
     8941 tiqwyregal ipqspdfqmi hegnnavlli attyeedtgt ftcrattsag tvetsakliv
     9001 kkrtgayyev paetgpgart gdkpfvslgt gkdvilepip ldkgeleipl kpgdetlpwr
     9061 kgrvhhrprl adttpwrktk pksrdtsldk lqatesqikp wteetvtlkk tpqqareipk
     9121 ekleevelkp akiekkeirr psletvdlkp ftkettketk ieekltrree idkeiyeeee
     9181 dsrflktsrk vdetvqrkkd evkpwteekv tlkkskpekk elpketletv elkpskigkg
     9241 evrkpslekv dlkpvpttvt ekvsekvtek teyleqedts lldvsrteev kkkrkekvee
     9301 kpekpeqakp wteekitlkk skpvvkeiek etletvelkp skigkaelrk pslekvdlkp
     9361 vpttvtekvt ekitekteyl eqedtslldv srteevkkmr kekveekpeq akpwteekit
     9421 lkkakhlpke ieketvetve lkpskitkse irkssvekvd lktvtketke itqktdyree
     9481 edttllevtk dtqvkktrre sipvekvegp kpwteekvtl kkakpekkev pkekpetvel
     9541 kpsrpqkadi ekpsledvdl kpipkkvtke ktekdkyiee edtsllevtk dtetttkvek
     9601 kvkrreevek kpetkpwtae kitlkkakve qkeirresie evtlkptkte krqvtketle
     9661 kvdlqkvtdk tqfveeedtt lmkvdkvekv eeervdkmat wrrdrkpkek apyheeedks
     9721 ildvkrdees tekrkevpvp wargkkkpte kapyheeedt slldvkkdee siekqkevpv
     9781 pwargkkkpk eekpelvpqq vqpeekpeee etlektqvpw lrdkkvarki perkeepved
     9841 iksvtlkpvr rerapeekek peevtlkpvk ripreeeeke evqlkpipkr kpeekpqeei
     9901 ilkpfqkpkp eveeqekpkl kpfekakpev peqekvqlkp fkrekpevpe kekvelkpfk
     9961 rekpeetlvp qkvpekpeee qpseveevsw rrkpkkkeai ipekpvekve evkvvedkvi
    10021 kkqeetiedf ksftlkptkr gkkleekeek eevtlkpvkr lpkeeetkae iklkpvekkv
    10081 eekeakplkp emaptvpeee kptevsevpw krgkkkpvek rveevtteek kveeeekevs
    10141 elswrkpqke kvkevkkeqe vedftavmlk ptrkekkpee keekeeitlk piqkvqkeee
    10201 kpeavtlkpv kvekpeavek eevpeektel pwrrgkkpak kivpqeevkp eeigkpeerv
    10261 pevkeevpkk pkvekpeeek levklkpvkr lekpeeakpe evtlkpvkrl pkeeeekeev
    10321 klkpikrekp eeekpevklk pfkrlekpee ekpeevtlkp vkrlpkeeee keevklkpid
    10381 rkkpeeekpe vklkpikrle kpeeekpeev alkpvkrlpk eeeekeeikl kpikrekpee
    10441 ekpevklkpf krlekpeeek peeitlkpvk rrpkeeeeke evklkpikke kpeeekpeik
    10501 lkpikrlekp eeekpeevtl kpvkrlpkee eekeevklkp ikkekpeeek pevklkpfkr
    10561 lekpeeekpe eatlkpvkrl pkeeeekeev klkpikkekp eeekpevklk pvrrlekpee
    10621 ekpeevtlkp vkrlpkeeed keevklkpik kekpeeekpe vklkpvkrlp keeeekeevk
    10681 lkpikkekpe eekpevklkp vkrlekpeee kpeevtlkpv krlpkeeeek eevklkpirk
    10741 ekpeeekpee iklkpvkrld kpeeakpeev tlkpvkrlpk eeeereevkl kpvkkekpee
    10801 ekpeeiklkp vkrlekpeet kpeevslkpv pkvvpekdee kpeeeapets ilpwrrgkkt
    10861 kkvvdfheev tvvpidqkki vsevhekett iveqkveake viekkvvvee vkpevveevt
    10921 evsevswrkp rkpkkpveek evvelkpvek heeivekvee kitlkkvkke vpeveekkei
    10981 qlkpvpkkpk eeevpekitl kpvkkekpee kpkeeeipel kpvklekpek veeteekeev
    11041 mveitrkkpk kkpeevpvek eevveteite iteekpeeis vlpwrrpkkv tekkieveei
    11101 kpekvedkev eevtettevs wrkqrkpkkp ieeeekpekv tlkpvkrekp eekpkeeipe
    11161 lkpiklekpe kveeteekee vkvevtrrrp kkkpeeiqre eievetkevv ddvevekiek
    11221 qeiqiedrkr krkqrtqvdi siidkdkkia prfiqklqpv iaepestakf tctvfgspvp
    11281 eiswyrneqe lhasekyimt vyettaslei tkvkeedagm yscrasnpag vatstvnlvi
    11341 fekeeegiap hfstpikplm veehkpalle cvvtgspvpe vkwyrgeqel kpkkgreitf
    11401 npetgeaklh ilepteedet iyrvravnkf graecranll innvvkvskp evlrapkitr
    11461 plpalvaerg kpltlsadfe skptpevkwf rngvelapse knviniyent aelfipevtk
    11521 kdggkyeirv qnpvgearss gsvtvkdrpd ktdevkaprf ieplqpqiva egevvimetk
    11581 vesyptasfq wfhetrples tpqvrivtqe nksilmikei kseyagtytc raenvggsvt
    11641 ctatinllet pweetvelvs ptfvkrlspv rvmdgesanl tcvvqgkptp kvewyhdskp
    11701 ikegkeitiv qdtegvcsla itevfpedag eytcravnpv geavcttsli veayeyvpds
    11761 eiassivats lttgqsgsee dllspketpl fdtdteesap eiikklpqli ptkdgeltrl
    11821 evkvkgkpkp egkwykqgve ivssqefqie efedgtsvlt iaetypddtg eivfeahnpl
    11881 gvsttmtyls vegivgtkey rkpewvthme emqealratq tvprfiqeit dvyvkegdta
    11941 vfecsysgnp tpdvvwyknd kmlmstqnlk vriydddkkt vltikhatee ddgayvckit
    12001 setglatska klhvtdvtde kkfyeeeeev eeqtievkpk ekpqkkklrk akevkekvkt
    12061 ervkvdkkev rkeklvpkeq peekkivdie etqilestdi ieekptevsq akkivpiqep
    12121 vlteataslk kiddekpkdm vpkpeerakr vieeregivv vsevttedma pdftveskvd
    12181 raqitseslq tvtvsevhte tsvqevrrae akqrkakktv vteeeifeer pkevvveekp
    12241 kkkkktekvk vreevtieei verqreniar eveeimemlh akefgpgeap lrelatigyl
    12301 vrqgvsvnei neslyrtenf palktpeaqn alvqlvereg hgplitqvlt eetttdesvv
    12361 aatvgfrafm rmielqhvtv eevlthfape afrprawevt eateveteer vtervdvvrk
    12421 tevhfmerrd erkavrtqdi rdikeyedtr qthttlrark drkpreetke drdegvqvve
    12481 ieeiedteqe kmkkkrkdve eieeeietvi vetdskgrla qkkkkrddle ekedveeeee
    12541 iiekvrkelk pkrpkigrde veleeieefd vtedksmpsi mvnvpsqrhq vvpldrtteq
    12601 apgkpeleka kltvdtital tehivpvqek eisdisrikp ierkaslsis pvepysttet
    12661 tvqastgefa dtfkptsyea tpgiipsegv qvsetlvsda gvtslsvskq eigrtaevsv
    12721 tlqeattvye tmvrekevpt edfvtpqtvk aedtilpqls lsvyevhegl tedkleplkt
    12781 vatkprvnvt aveplvveev raedkpgkyy pelivpteva tqsvisqrqr vteemhapek
    12841 egeyipgrlp psqvaqigis ygnetavvqq nvvqesegvf isekktetfe attnvtvleg
    12901 vsvstvqtqd tetsltieet kkavadlnvi emtpavtvet vpsekekeyq pgdkpdtkta
    12961 etsissleig siqstvvqes egvyrpdekp tkalaetsir peehvmisei qtadypsdfr
    13021 eelkyvqesg tvsvqlteak miqetlthdr eermeemvkp eermvdtsyd vnrsvevfqt
    13081 tsvekeadlr vfempeshrg ktvpthpvvs levemtqped nlgevkkevp ssgtakvehv
    13141 slqetivget vvaenlapve kdkapetkva eiamnevesl httmvvvsek eteytqvsdv
    13201 kgayasteft tqvapmleev rshspteeyl pdqkptsgia qpshvplesi tvavqelaek
    13261 edlyktdvrp dqktanvalt eltsisipev mqvitassae tlkketkmke ekgrveveel
    13321 sstiisevis netedvfttk atpkdqkadf nisgreaaet tqvtamteae dlavkrpeeq
    13381 kgkpqvdelt pltvshvetg eaekelptae vpkektaqpn ifgrdvaetm qvltvtsaee
    13441 laapkapegq kgkpqveelt plsvsqvvsq eaesalpape apterkaqpd mtgrdiaetm
    13501 evltmtsadi laedvkpteq kgmpgleefv plsvsqtvst ekedvlpspe apttktaqps
    13561 ltgrdiaetm qvvmmtseke litalapeth kgipnveelt pltvsqtvsh eaedvfekta
    13621 apsevtakpa fsgrevaqts emmivtnvge lekqvlpdeq kgqaqldelm pltvsqvisg
    13681 eveeglpeae kpkektaqpg ilgrdiaett qvltvtsaee laapkapegq kgkpqveela
    13741 pltvsqvvse eaegilpape apterkaqpd mtgrdiaqtm evltmtsvdt ftedkkpdeq
    13801 kghpeleefv plsvtqtvsn emedvlpspe apstkiaqps ltgrdvaetm qiltmtseee
    13861 litaqtpeqh kgkpnveelt pltvsqtvsq eaeeilekaa aptevtakpa fsgrevaqts
    13921 eimivtnvge lekqvlpdeq kgqpqldelt pltisqiisg eveeglpeae kpkektaqpg
    13981 ilgrdiaett qvltvtsaee leapkapegq kgkpqveela pltvsqvvsq eaegilpvpe
    14041 apterkaqad ltgrdvaemm evltmasvdt ltedkkpaeq kgvpgleefv plsvsqtvst
    14101 ekeevlpspe apttkvaeas ltgrdiaetm qvvtmtseee lisarvpeqh kgifnveelt
    14161 sltvsqtish eteeilekaa algevtakpa ftgrevaqts etlivtnvge lekqilpeeq
    14221 kgkprvdemt sitvsqvvsg eveerlpeae kpkektaqps mlgrdiaett qvltmtsaee
    14281 laapkapeeq kgkpqveela pltvsqvvsq eaegvlpvpe apterqaqpd ltgrdvaqit
    14341 vvmsmasadv middkkpeeq kgvpgveefv plsvsqtvst ekedvlpspe apttkmaqps
    14401 ltgrdiaetm qvvtmtseee litalapeth kgkpnveelt sltvsqtisq etevileksa
    14461 apseatakpa ftgreiaqts eilavlnvge lekhvlpeeq kgkpkleelt pltvsqiisg
    14521 eaeeglptae vpkektaeps mlgrdiaett qvltmasaee lmapkapeeq kgkpqveela
    14581 pltisqvvsq eaegvlpvpe apterkaqpd ltgrdvaqtt vvmsmanvdi mieekkpeeq
    14641 rgipgleefv plsvsqtvst ekedvlpspe vpttkmaqps ltghdvaetl qvltmvseeq
    14701 lttkapeeqk gkpnveelts ltvsqtvshe aedilekava pseatakpaf lgrevaetsq
    14761 vltvttvgel qkdvlpeeqk gkpqleelmp ltvsqivsge aeerlpeaek pkektaepsm
    14821 lgrdiaettq vltmasaeel lapkvpeeqk gkpqveelap ltvsqvvsqe aegtlpipea
    14881 ptertaqpdl tgrdvaetme vltmasldtl iedkkpaaqt gapgleefip lsvsqmvste
    14941 kedvlpspev ptekmaqpsl igrdvaetmq vltmisamel ttghapdqqk gvpnveelts
    15001 ltvsqtvshe teeilekava pstvtakaal sgrevaetsq vltvsnveef eksilpegqk
    15061 gkprldelts itvsqvlsne leetlpspek psekiaqpqm igrevaeqte vltvtnveel
    15121 kklekpegqt gkpdveemsf itvsevfste mekempspev pkertalpql ssievaette
    15181 vltmisaeel lkskapeeqk vqpkldeltp ltvshvvsee aeealptaei pkektaqpsl
    15241 lgrdvaetsq vltmasaeel laakvpeeqk gkpqieelap ltvsqvvsqe aeealpsaev
    15301 pvereaqpsm tsrdvaqtml vtsmsnvdim aeskipeeqk gvpgleefvs lsvsqtvste
    15361 kedvlpspev ptekmaqpsl igrdvaettq iltmisatel ttdrapeqqk gvpnveelts
    15421 ltvsqavshe teeileklea pgtvtakaal sgrevaetsq vltvsnveef eksvlpeeqk
    15481 gkprldelts itvsqvvsne leetlpspek psekvaqpqm igrevaekte vltvsnveem
    15541 qklekpegqk gipdveemsf itvsevvste aekelpapea pkerkalpql sgievaekse
    15601 vltmtmteef akpkapeeqk gkpqleells lsvsevalge vekglptpdk pteetakpsl
    15661 lgiqvaeksq viassitgel qesikpdtkq iipeqipfes ieqsqtltqe restfvvekt
    15721 ttsataevsf rvseslevtq vtatekeske vikgvakeas aqadvierkv alktevqped
    15781 masefkvkkp egktakgvde iqqgvivteh ltahefesel pesvlpfakl arvsiqadhl
    15841 ehivetteap aqqhhititq httkhdlpqq qsiesdtevi eeyttkfrrg stedataavk
    15901 tkktivrkkp kttgeedivv vieeelddik pkipsipkkq fmpieevttv sdveeivsar
    15961 ideaeeivpk eeekvevtit evqdtdkkkk vtkkqfvrrr gktrqvteei tveeegkape
    16021 ttvvtkpvei ieedieerpk iekeeyvvee vpeektiplk prkkltptvq qveeqvtvte
    16081 eitkegkprk ttkkkvitrk gkeqqvtevv tveeqgkapi tsvtegpvee vveeiiqklp
    16141 apkyakpsev eevreqvtvt eevtrlgkpk kttkkkiikr kgkeqqvtev vtveeegkap
    16201 tttvtegpve evvieeikel pipefvkpte veevreqvtv teeltkmgkp kktikkkiik
    16261 rkgkeqqvte vvtveeqgka pvttvkegpv eevveetitp llapeyakps eveevreqvt
    16321 vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp veeiieeeik
    16381 pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv
    16441 ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    16501 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    16561 tregkpkkti kkkiikkkgk eeqvtevvtv eeqgkapvtt vnegpveevv detvkalpap
    16621 eyaepsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    16681 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    16741 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    16801 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    16861 reveevreqv tvtqevtreg kpkktikkki ikkkgkeqqv telvtveeqg kapvttvteg
    16921 pveevvdetv kalpapeyak pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    16981 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    17041 kktikkkiik kkgkeqqvte vvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    17101 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvmegp
    17161 veevvieeiq elpipefvkp teveevreqv tvteevtrlg kpkkttkkki ikrkgkeqqv
    17221 teivtveeqg kapvttvteg pveevvdeti kslpapeyak pseveevreq vtvtqevtkl
    17281 gkpkkttkkk ivkrkgkeqq vteivtveee gkapkttvte gpveevveee vkpllapefv
    17341 kpseveevhe qvtvtqevtr egkpmktikk kiikkkgkaq qvtevvtvee egkvpvttvk
    17401 egpveevvde tvkalpapky akpseveevr eqvtvtqeit klgkpkkttk rkvikrkgke
    17461 qqvtevvtve eegkapettv tegpveevle eeikplpgpe fvkpseveev reqvtvteev
    17521 tregkpkkti kkkiikkkgk eqqvteivtv eeqgkapvts vtegpveevv detikqlpap
    17581 kyakpsevee vreqvtvtqe mtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    17641 tmtegpveev veeeikplpa lefvkpseve evreqvtvte evtregkpkk tikkkiikkk
    17701 gkeqqitevv tveeqgkapv ttvtkapvee ivdetikelp alefakpsev eevreqvtvt
    17761 kevtkegkpk ktikrkvikk kgkeqqvtei vtveeegkap kttitegpve evveeeikpl
    17821 palefvkpse veevreqvtv teevtregkp kktikkkiik kkgkeqqvte vvtveeqgka
    17881 pvttvqegpv eevidetiks lpapeyakps eveeireqvt vteeltklgk pkkttkkkit
    17941 krkgpeqqit eivtveeegk apvttvtegp veevvkevlk plpvpkvvkp seveeireqv
    18001 tvteemtkeg kpkkiikkkv ikrkgreqtv qevvtveekg kapvttvtvg pteeiveetv
    18061 ktlpapeyak pseveevreq vtvtqevtke gkpkkttkkk vikrkgkeqq vtevvtveeq
    18121 gkapvttvte gpveevveel lkpliapeyv kpseveevre qvtvteeltk egkpkkvikk
    18181 kvikrrgreq qvkevvtvee qgkapvttvt egpteeivee tikalpaped akpseveevr
    18241 eqvtvtqevt kegkpkktik kkvtkrkgke qqvtevvtve ekgkapvttv tegpveeive
    18301 etvkalpape lvkptevtev peeltvtevv tkegktkkiv kkkvpkpkke gieapeeape
    18361 evpveetkki iklqrlkvsk festkldiqe tslgkpqfaq iklrktpvak rpskkdekfp
    18421 rillrsriia iewppspknl kvdtfepiel qngilsrnvd eaaalpkpkk kkikriekei
    18481 aklekldqef eelkkelple kleepileek perkvkkkkk pedkakegvk pqlmrisike
    18541 qkpikpkiae patplfaqik lkkapakpkk kpeeevskfp killrsrita hewppsvkyp
    18601 aisvlepnyi qngilsrtme ealklkkvkh kkmklpekel telekldqef eelkkvplek
    18661 veeitpyerk pkpekpeeek pkklvigkgk rrpaeeedle tvqlkkipek ipsapeeaek
    18721 vpkkkdevdk pkkekeekey pelkpfepyd ieapevelee rekpeypepe kpkekkvpgk
    18781 kpkkerktpa qeteavpivp gvpkpkepee eeeikrripe rpppteepek iklkpwkkge
    18841 kpeeeekkpe dyikfvpkdd divetvevtt tveetpdkkq rkikkkktit kpkdekpkii
    18901 eettieeegf epvvtveefd vdkyeavpve svekaapvee ikeeivtsei iteegtkktv
    18961 rkkrvtkkre gkervteevt veeegkepit atielhedti seiheqakhk rptkpkadek
    19021 laedvtektv eqqeiieevk evkptkkvik kekpvkakde vteevveeii ieepekqeak
    19081 piveeeetvi itehdvrkap kkkkevvike eeeifetiet pthkiirkki rpmkkgekev
    19141 vveeiiqkpr eetvemeeqq ivevvetptk kivkkkkevv kddgvpeelv eeiiiekpqk
    19201 kkakptveee kkkvkvtkvd tkkapekpee vveeeeeiie tietptkkii rkkpkdkkdk
    19261 iieeviqkti eeakameeqe ivevietptk kvikkkttti eegkapeekv eeiviekpke
    19321 vieeeeeiie tietptkriv rkkpkdkkdk iveeviqkti eeaktmkeqe iievietptk
    19381 tvikkkkati kegkapeeev eeiviekpik ekakkkilpk egveitevit etsveeliee
    19441 elkpeeakps eeveeekvki tevaikkvpk kkkpskavea eekeevveei kpekpkeeka
    19501 kptvtekegi evteviseva peklpeeakp kkkkateike teekvitkkv kvkeapkeav
    19561 pgeeeqpaee vdelvpekpk ktkakkevtp qdsivttevv pitkeeelev ekpeeekatv
    19621 veekeekakv eekkvtvkka pkkklkptea eeeqveevve eitpeepkkr kakkavipke
    19681 eiqttevipg itaeeipeee kpeeekavpk eeekveeeak vtevsvkkke rkkkekpvtn
    19741 gevieekpkv keipeekpke evpqvpeeke kpeeekpkev kpkekkpkee kpkkpieekp
    19801 eevkpeeekp evkpeeekpk eekpkkkkpa ekkpkiseee rpedkveeip ekkpeevktr
    19861 eeiipkdtvq ttevipetva eeiptkkpke rkavpveeke ekveitevpv ekapekkeek
    19921 vpeekpteaa pekpkeakvm kkkkkkpakk deieewvepe yerpvlepmp ekivwepkkk
    19981 kekkplpesl qklvpqkier kemkptklky sepaetvqfa siklkkvavp kkkeadetkf
    20041 pkimlrsrmt fvgdyppelq ipkityleen pvqtgilsrn teealkvlkr kvkkvklsek
    20101 evtqlekldl efeelkkvpl ekiedksvye rqpkkpaekp eepkkvpigk gkvpeeekve
    20161 pekvklkkvp ekkpeeveep lkkpkkpepe eevkpekkkk pkvklehdel kpldfdrpel
    20221 ekyepeeyek peepapepvp kpyekepkkk pdqeveqipl kkgkpkspep deepevkfri
    20281 ptkekpeeep eqitlkpwkk ekpeeekeke ipkkeeekvp kppkeepedv tvqrkaktkk
    20341 pkekpkeeke eevtvkkpee ekpeevaeev tlkkepekpv eeapaevtik kpvveekeve
    20401 eeetitevil kkkpekpeev veeavvkkpk kkkpvkeeev veevtvtkvv teeekeeekv
    20461 vikkpkkksi peiteevtee itlkkpeeka peevteeikk pkkkkpvkee vaeeitikkp
    20521 vveekkeeev peevtlkpkk peekpvieev teeitlkkpv peevteevai kkpkkkkpve
    20581 eeaadeitlk kpvvvekeee eiteevtlqk kkkpekkpvp eeiseeitlk kpvpkeeeka
    20641 peevteeiai kkpkkkkpvk eeaadevtik kpvveekkee etpeevtlkp rkpkekpvpe
    20701 evkeevtlkk pvpeekekvp eevteeiaik kpkkkkpvee eaadevtikk pvvvekeeee
    20761 iteeitlgkk kkpekkpvpe eiteeitlkk pvpkeeekap eevtekievk kpkkkkpvee
    20821 eaadevtikk pvvvekeeee apaeitlkrr pkekpveeit eevteeitik kptpeekeka
    20881 peevsekvev kkpkkkkpvv eeaadevtik kvvveekkee eapeevtlkp rpkkksvtee
    20941 evaevtltkp kpieeekkpe eseltikkkp kkkpvieeea aevtikkpvp vepdeapeei
    21001 tirkkkkpek kpkvteevee tavtikkara pekpteeeei ttdvqikkkk pqkkveevaa
    21061 deltikkiee veqpeeeeev qeftfkrkpp klppkpieei yedvtlrklr pkrksrpdik
    21121 eitetenvtf rprttktked veqefkisln tyeeedisms gkvrlkpkkk pltyseetge
    21181 etiriiqeve ddsgpiieei iesdeegkde ysieeldtde mrlplrrkkk kvpkpykved
    21241 ieegdvklkl krerkysiee adeslalklk skrrvstyde eeatlsitre ediseeveie
    21301 yvvrdgdtmf sicsyvaetd eainlveger vyvidhtnqd wwfvkkhlte ekgwvpaqyl
    21361 lnevhytlyl qrklhekidk lpvfekpapg dkssapkfve klqpihtpdg ytiqfecqve
    21421 gvprpqitwf rqtaiikpsp dfqmyydddn vatliirevf pedagtftcv aknaagfass
    21481 tteliveapl sdhgsdltgp srkslsress ladilegipp tfsrkpkaky vdegedvile
    21541 crlvavpepe itwyykdtqi ttkeniiiat esdmhmycsv ikitkvqkkq egkytivarn
    21601 regeatieip mkvktgksep peileplqsy vvregetvvl stqivgnpap kvtwfkngkp
    21661 lkgltpkqdg hvntltliqp qvsdtgeysv vatndlgtae tkatltvein lkpslainfq
    21721 qhtfehfsvt keipsgapep plfterfqel tvpekgtfkl vakvtgnpvp evtwlrnnkp
    21781 lekspnitet ydgenivlei rnansevdtg dykcvasnpv gkashgarvt vdvdkvtftk
    21841 tlekevvvde yktleltcet shtvstkwwh ndkeisgmdh reiieegrvh klvikktnpt
    21901 degtykctvk nqstssnvtv katkpefvkk lqdcevkerd vaileveits qtvdvkwykd
    21961 geqlgpgkek lefvkegtvr kllirstsvh degeytcsll dqectaevtv ielppeiitk
    22021 mqdvtiarge ratfdieltk gdalvrwfkd gqelqfsehv rlsidgkrqk lkiydtesed
    22081 agvyscevgq qkssakltve epavefitkl pdvtlvplns davfvielsq dvpvtwmrkt
    22141 qvikespkyt iidegkvkkl ivkkcttedi seytamvanv ktssrlrvev ietppkispd
    22201 tprkykvrkd edvdivvkfs gtpkpedewt vngrvvtksk raiptidees avltirkiqe
    22261 edvgdytlkl vnsvgetsie invvivqvps apgapeplet tndsvtlhwk kpdsdgnspi
    22321 veyilehqek tettwtkite titetthkvt klttnkeytf rvtavneagp getsptspyv
    22381 kiskpstaep pvileplksi viglgetvtl scviggtpap eitwlrndei fedtsityen
    22441 hitkytiret tetssakftv kaknnvgsae ttcelkiqes pkiiydenlt nqklpmngqw
    22501 kieiqitgfp kpevtwtkng kevdekrvsi hreertstvs isslvredta tymakaenqa
    22561 gsssvdlhlk vidkpskpqg pvvfkeirqd rvtlewrppa ddggieleky tiekcepgkt
    22621 wvkvvdidke vesfcvhklq qnaeykfrii arnavgasep lesdtvkmrt sfeppgaprg
    22681 plevsgmtkt sftikwqppe ndggtpitdy iieikeeskk twqkigstkh etthfsvsdl
    22741 ktdvpynfri taknsvgsgp pyvaeepiap grrikitklt essmyallgi fpptkivisk
    22801 tppsspqhvh vtnitsksat lswsppastg gteltgyvie krpligkgar wtkvvtldat
    22861 tlqycvenlk eseflfrifa ennqglsvpa nsepvtlkth asvpspptap leirqiaant
    22921 vviswgrpds dggaplegyk vairdtkrtm wmevarvnte iqklnirdlq enhmylirif
    22981 arneiglsdp leseepfkii paseltvvep iaeatekget asisfstent sswlrehnmd
    23041 adihsyarar llrqdeyffr iwhyakklfe
//
LOCUS       XP_034182871           22969 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X12 [Osmia lignaria lignaria].
ACCESSION   XP_034182871
VERSION     XP_034182871.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182871.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..22969
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..22969
                     /product="sallimus isoform X12"
                     /calculated_mol_wt=2595687
     Region          78..277
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            156..161
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <458..1112
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <705..1015
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1284..1373
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1301..1305
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1314..1318
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1339..1343
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1353..1358
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1366..1369
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1385..1474
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1402..1406
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1415..1419
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1440..1444
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1454..1459
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1467..1470
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1484..1573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1501..1505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1514..1518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1539..1543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1553..1558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1566..1569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1596..1685
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1613..1617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1626..1630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1651..1655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1665..1670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1678..1681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1711..1801
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1728..1732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1741..1745
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1767..1771
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1781..1786
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1794..1797
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1823..1911
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1840..1844
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1853..1857
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1877..1881
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1891..1896
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1904..1907
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2079..2168
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2096..2100
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2109..2113
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2131..2138
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2148..2153
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2161..2164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2175..2265
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2192..2196
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2205..2209
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2231..2235
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2245..2250
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2258..2261
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <2900..3173
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          3886..3975
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3903..3907
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3916..3920
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3941..3945
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3955..3960
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3968..3971
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4132..4219
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4149..4153
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4162..4166
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4189..4193
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4203..4208
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4329..4418
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4346..4350
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4359..4363
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4384..4388
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4398..4403
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4411..4414
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4454..4544
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4471..4475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4484..4488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4510..4514
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4524..4529
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4537..4540
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4553..4643
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4570..4574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4583..4587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4609..4613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4623..4628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4636..4639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4700..4791
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4717..4721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4732..4736
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4758..4761
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4771..4776
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4784..4787
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4833..4925
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4851..4855
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4866..4870
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4891..4895
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4905..4910
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4918..4921
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4972..5064
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4990..4994
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5005..5009
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5030..5034
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5044..5049
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5057..5060
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5106..5198
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5124..5128
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5139..5143
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5165..5169
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5178..5183
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5191..5194
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5240..5332
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5258..5262
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5273..5277
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5298..5302
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5312..5317
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5325..5328
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5374..5465
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5391..5395
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5406..5410
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5432..5435
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5445..5450
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5461
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5507..5597
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5525..5529
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5540..5544
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5565..5569
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5579..5584
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5592..5595
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5641..5732
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5658..5662
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5669..5677
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5698..5702
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5712..5717
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5725..5728
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5774..5865
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5791..5795
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5806..5810
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5845..5850
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5858..5861
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5907..5999
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5925..5929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5940..5944
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5979..5984
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5992..5995
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6041..6133
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6059..6063
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6072..6078
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6099..6103
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6113..6118
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6126..6129
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6173..6265
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6191..6195
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6204..6210
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6231..6235
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6245..6250
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6258..6261
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6306..6398
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6324..6328
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6337..6343
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6364..6368
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6378..6383
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6391..6394
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6440..6532
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6458..6462
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6498..6502
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6512..6517
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6525..6528
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6572..6664
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6605..6609
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6630..6634
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6644..6649
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6706..6798
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6724..6728
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6739..6743
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6764..6768
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6778..6783
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6791..6794
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6838..6928
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6855..6859
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6870..6874
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6895..6899
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6909..6914
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6922..6925
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7005..7065
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7009..7013
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7034..7038
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7048..7053
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7062..7065
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7113..7204
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7146..7150
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7175
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7185..7190
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7214..7305
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7232..7236
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7247..7251
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7269..7276
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7286..7291
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7299..7302
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7347..7438
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7365..7369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7380..7384
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7402..7406
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7419..7424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7432..7435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7485..7576
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7518..7522
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7543..7547
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7557..7562
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7623..7714
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7640..7644
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7655..7659
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7680..7684
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7694..7699
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7707..7710
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7760..7850
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7777..7781
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7792..7796
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7817..7821
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7831..7836
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7844..7847
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7893..7982
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7910..7914
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7925..7929
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7950..7954
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7964..7969
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7977..7980
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8036..8126
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8054..8058
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8069..8073
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8091..8095
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8108..8113
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8121..8124
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8174..8262
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8191..8195
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8204..8208
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8228..8232
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8242..8247
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8255..8258
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8497..8587
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8514..8518
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8527..8531
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8552..8556
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8566..8571
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8579..8582
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8601..8690
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8618..8622
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8631..8635
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8660
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8670..8675
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8683..8686
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8711..8800
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8728..8732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8741..8745
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8766..8770
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8780..8785
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8796
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8812..8899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8826..8830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8840..8844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8865..8869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8879..8884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8892..8895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <9016..9669
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <9722..9875
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          11150..11239
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11167..11171
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11180..11184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11205..11209
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11219..11224
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11249..11340
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11266..11270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11279..11283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11306..11310
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11320..11325
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11333..11336
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11355..11444
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11372..11376
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11385..11389
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11410..11414
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11424..11429
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11439..11442
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11457..11543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11474..11478
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11487..11491
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11512..11516
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11526..11531
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11539..11542
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11560..11650
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11577..11581
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11590..11594
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11616..11620
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11630..11635
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11643..11646
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11698..11784
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          11717..11721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11730..11734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11756..11760
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11770..11775
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11822..11913
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11839..11843
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11852..11856
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11879..11883
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11893..11898
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11906..11909
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <15401..>15553
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          21207..21259
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(21212,21214,21220..21221,21238..21240,21253,21255,
                     21257..21258)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          21295..21385
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21312..21316
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21325..21329
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21351..21355
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21365..21370
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21378..21381
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21419..21512
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21436..21440
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21449..21453
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21474..21482
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21492..21497
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21505..21508
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21520..21606
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21537..21541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21550..21554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21572..21576
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21586..21591
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21599..21602
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21640..21730
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21657..21661
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21670..21674
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21695..21699
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21710..21715
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21723..21726
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21743..21820
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          21753..21757
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21764..21768
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21789..21793
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21803..21808
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21824..21909
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21841..21845
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21853..21857
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21879..21883
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21893..21898
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21902..21905
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21914..21998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21931..21935
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21943..21947
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21968..21972
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21982..21987
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21991..21994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22104..22184
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22112..22116
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22125..22129
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22150..22154
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22164..22169
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22177..22180
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22188..22279
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22188,22253,22268)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(22269..22270,22272..22273)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          22290..22376
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22307..22311
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22320..22324
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22342..22346
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22356..22361
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22369..22372
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22400..22470
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22400..22403
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22412..22416
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22436..22440
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22450..22455
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22463..22466
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22474..22566
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22474,22540,22555)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <22544..22859
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(22556..22557,22559..22560)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..22969
                     /gene="sls"
                     /coded_by="XM_034326980.2:205..69114"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mvdghdsmet tensegcrek esrtvvesne dapqqttspt ttistiavqs getrlviall
       61 qsgewlrlkv levqpeltkl gasveeatel ssahdevllr lqskqspvee llrqadqlis
      121 nqrpraevya amaetlgqaw rdvnellerr kqildcnvlf qcraeecres mralemacnd
      181 tllpieieav knflskihdl rknmlealmg alqegknlld rlkeianegt ldsrpdrikl
      241 eadhavlkve kwleelhdrr rlieasfrsr ktqleqclal allatdlrdl eeilndriaa
      301 lssscdqlgd sassaelllf elkklqaeak efqdrsikit ksterlvssg hfageqateq
      361 ayailgaaad yvndldqyes llnravaffd sarsaltkld qleiqlvtte hpqystrlar
      421 fhaqtvatie dvtakplaeg yalldvtgrg apgaegvkrt vedlenrkir lmerctahek
      481 enleisrmin tflekhdelr kwlmsipeaf lqghqdmgsd vpmaedfcrl hrqlsndler
      541 rtdevehlef eilpirekle esqklelksk veelqrswsk tkelvanrid lgsvylqfhm
      601 vadeltreve sieselksna dvlderkvee lgrrwkdlqp lyvkltgsgk afldaaikid
      661 dpyldvprac lcvqtllekf anrqltvtes wekwrtsiei lrerrieher kieestrtle
      721 wvskfseqly pvitsqsaet tmilkdlags kqrilpelnk avseldsrik gintlaqkge
      781 vhvdeeilkr lhqmhedlrk tardyeille slisifesie eveqkietlk iqvervtslk
      841 kifeveaafs eidatkwsis drlghiklri edaivrikqq eppgagvkdi eklrraldsl
      901 tsdfeifwsq tttkveehrr tctfiedler idgelknlne hlknvdarmg enlqtakats
      961 asfvqfektv tileqriekf vqtteesiki ltpqivddis llkerwrnlk krmeetkkrm
     1021 nlcidyfvll eeakewnreg skllvviark atmvkapkda tdllheieny lkpgeevqer
     1081 rieklkelst ivfgterlpq fnevivenrq mldsftvvss elrtlvqnlk naedlqeklr
     1141 iekqeadekl qaakvemaaa aaareeaena rkiaeklaae tlekaatevk tlkeereaqt
     1201 msappsfsvs aqtekiektd esyvqetvts avttkeihil qkteiektvp liqrepspgk
     1261 iivteeihek sekiekddvp plapeftipl ndatvqegek fsfqcnlvgh plpevvwykd
     1321 gisilnnpdy lttyvrdvct ltieetfaed sakytcrafn iagsaetsat ltvketapee
     1381 qpsppvfvke lvpstaiegs shqidcvveg nplptvqwfk ndvnidnspd yvitynngea
     1441 vlkfeevlpe dkaiymckat nrlgqastsa slnvqrtect tekpqfvtpl snamgragqr
     1501 vkleceargk pmptltwyhd grsveetmnl kiqtdggrss liiseafakd agcymvvakn
     1561 dageatvscn vsvkgrlphe tsdsefacsd mepvvpkiqm plkdlrvqeg rsvrldcviv
     1621 gqpepeviwy hddqpvkesa dfqllfqgdk ctlliheafl ddaglykvva insggeassq
     1681 ctltvtpvsv thelektrte veetvtagsp pkfvklptds liaegenaif ecavvgepkp
     1741 elkwfsdsge iseseririq rkedgtsilr ilsaipedkg nyvvkafnah geakafarlv
     1801 vrslgdfrrk eefvqmeekl iaptfkerfe drkvpdgvst kfecivvgkp spkiqwlfnd
     1861 rpvhgkdflv svsgdrqvlt ipetgsthvg tiscvaenaa gkatctarle iggsevqrdk
     1921 kevveekvye lveripgdem hassssdkhf saekseigge nkvltkmset itesstthkt
     1981 lkkeyisssm ssstgapgqe ptsvcvkstv hsteqsssad gappvvqsyk veehekiiqd
     2041 qpgeirqert vvvsqdeegi krdikatqip kpvrkstapr fvspvtgmiv dqgsdivleg
     2101 iidgfpqpvv iwskngqelk tkdsmtitys hnhvrlelkn vnvkdagryt ctasnevgsa
     2161 sstadlvvkk tifppvfgrr lqaqlvkrgd rvnmeveitg tpeptvswfk ddipikerpp
     2221 elrikqqgnc ymlimekaak ehagkymvra tnaggeaqsi adfavfeptp dtmvevhktv
     2281 iydvqdkklm qsdekkaevp sskhltseqv sttkiqpaat ikttptamst smaqmsrtve
     2341 kikepdtelc rsekisstve shqmerkseq kyhmklehkm psivepkteg ktvikevire
     2401 evrssepkst lieekienen ietsriakkd alsffesmtr etetipkgpk emiklvddad
     2461 ghevkvgklt knyekstsfq evkkpevkpp ellttkkavh eiftkleqgs ipsrgidnkl
     2521 fdfpyepykp tmetkktvve etissgsssl qssmtmskme skreskmesk ssellsegfn
     2581 lvpepppeig ympkpeevkk krpevsikak qlqesfdktl spidapiggv kifpspspki
     2641 letpkvapps fsmppppfql ekkeiveevc ikkdvhekrd pqplvrpisp srpwssssdv
     2701 etrsqmstdl seyrchsaas shqeilrsss prpsadglam ekswakkcad strkswppqe
     2761 dtpikykqew qmpgedykts skefkrevee tpeggikkts messstterr swsskqetmd
     2821 kmskrapspp kiqpiiynae tikvdhtvnk ieekslyeky isecdvkksq ksekleeyss
     2881 kkwtgaedlk apglvrslet pkkptpplyr pvepiptdqi ilepgpppei gyipapvvre
     2941 kkieqiektl evslehkpak ippggirtip papkkeeppa lppkdvritp pplpakqptp
     3001 veplepfpfk pspsptiktp kvavpaptps kfvkgsfgve sdyesdmdsh mkakwrpyes
     3061 dneepryrrv qppvpkqprp rstepeplpp skfevpsest glprpvltke qqekmykktt
     3121 tfkrqeketk qqkyqvvqpp pvelkpgspp iyvqpatksp tpksppakkp espkfkvktf
     3181 qqesgymadt deplqqkssv aslsksfgkq essssmsshm esrtsysesr seyfesksyd
     3241 srqqqqkkes fapsapssvk qppqqrssfv eksygssavt ssprfmsake tlyttdqshk
     3301 kfeapkkilp pspspsrfvk sefresdyes dydgripplw kprgyesddq sfrtvkpnla
     3361 gpgkpkvqre dtsrlieeas traqkaktfe kreervatkp ktvllpgspp eiayappqpq
     3421 ptyyearsgi pfhnaigtet kktvrmdest ensrrivtve qtsrvikfgd nqksatdfds
     3481 fdsssaqkqk thykvptpkk fvqgqfresd yesdidtrik pkwappesds eepryrkvqp
     3541 pttkttrsss apvrqehvvs pmefdtgptv lrkqtsmsql rderkfdgqv krqassrqee
     3601 tlkpgsppef gyisrsdvkk aanhvasrhm sdmtssfksk tekfvtdiqs dlkqgkpilk
     3661 qpvdgrasda deprtyrees rlsehgtkqi dpdtgliyfk ydfgyefgiv lpgegkktvs
     3721 stsrsvqgqr rasdievpiv hefttrkeng fakrsassss tarqgkpagk fsgsktvkwe
     3781 ptseseyses edirnarkrh seggntmtap slvipcspsp srwdhttpsp lslspslpsl
     3841 spryssatgp psnvdsagsp wpttngvass keviqpylei lpkkaplfit plrdiavvsg
     3901 qtarfecivq aepqpnilws kdgriienst syeihyrngv crltivrafp edagtyacta
     3961 tnslgstvts atllvpgvps pfygtyrdqn lsersaespp kyvalrdlfl pqesdvktas
     4021 snrqqqhnss tfeyawsvnr syaggryrek enateecvei tsrnpiarrl eysdfqands
     4081 frdvadfcfd reystcnpsi asprntvqge ivmqttptvp aftgrpgdps ppvfeqifkn
     4141 arfaqggnai fegrvrgnpk ptvtwthkga plleswkirm syddktgavt lqinqigpgd
     4201 egeytcsakn qygeaicsvy iqpegfgppp qqqiggyrke yaqtfqsteq ksqtgsqsfq
     4261 qrsyqqtidk rsyvngtsts iedfkvdtfe yrllretefr esitrrfvge sdvqistvvd
     4321 rslgpvappq isqkprnskl iegsdavfta kisgnpkprl twfkngqrir esqrvemshs
     4381 nqqatlrirv alpedsghyt llsenpqgct vssaylaies sdqvdqayqt presvktpqv
     4441 estgesdsgk vlppnfvrtc tdrdategkm trfdcrvtgr pypevtwyin gqqvandlth
     4501 kilvnesgnn slmitnvsra dagvvtcvar nkagetsfqc nlnviekeqv vapkfverft
     4561 ttnvkegepv vfmaravgtp vpritwqkdg vpitpgpdvr istdgsgast ldipcaklsd
     4621 aawyqctaqn vagstatrar lfvetpkgaa peprrlnlpr ptkviepepa pgpeviylrh
     4681 verakpylpp peedrvyppp rfiiplrdvh qieggrihfe ariepvgdpt mrvewyvngr
     4741 aldassrats ifrfgfisld lisiilqdsg eyvcrvvsst gvaesratls vtpratieqa
     4801 sqhpdslqyi qqledyskyq rqesveeiss qrpafirplq dlgelqegrn ahfeaqltpv
     4861 sdptmkvewy kdgrpitass rittifnfgy vslnilhlra edagsytvra vnrlgeavss
     4921 aslrvyarts vttdlgipeq qryieaaeel eayqqamhqk yvqeqpepts ppefkspikd
     4981 qnsireggfa hfearlepvg dsdlrvewlk dgrpveassr ittffnfgyv altikyvtih
     5041 dvgiytcray nrageahtta qlsviskndv iydsqhptgl qkiqtledss rysrqvqeet
     5101 qvtqaprflg tlkgtnkive gqrahfearv epqsdltmti ewyhngkait aanriqtyhd
     5161 fgyvaidilq vrpedagtyt vvarnalgea rlsatmvvet rssvdtssmh rtsyektqrl
     5221 eeskfvepqy hieeiskskp ifvqplsdpk pvsegknihl ecrlepmgdp tmrvewfqng
     5281 rpvtvgsrfr tyydfgfval divhstilds geytvratnh lgtahtsacv rvigksdivt
     5341 etqheqsleq iqmledaary rktqqeevtv mqapqftrpl hnietveltn vhlecrlqpv
     5401 gdatmkvewf vngrpvktgh rfrpsyefdy valdilgvyp edsgvytcqa rnqlgeavts
     5461 csvrvhakkd lllesqhpeg leriqyleda srykrhelvd evvkvkprfv tapknqenlh
     5521 egqhahfeck lepvtdsnlk vewykngrpv tighrfrpih dfgyvaldvi dliaedsgty
     5581 tcravnlvgs devscslscr staqvltdtk neqgleqihy ledrskyqrr edveetttqa
     5641 pifttslnnv eikegqrahf ecrlipvsda tmkvewfhnn kpvkagsrfi etnsfgfval
     5701 dimyaypeds gtytcrakni igeaitsata nvhskksiyt etqneetlqr lhhledtsry
     5761 qrkttteeiv tqapvftmpi kdlkvaenqa ahfearvipv gdsklkvewl rngvpiaasn
     5821 rvttmhdfgy valnmkyvnp edsgtytcra vndlgeavts atlfvqskaa lqfesqhesa
     5881 lsklqaledt sryqrheeee ivvkekpsft vqlngptnlv egqsahyecr iepypdpnmk
     5941 vewfhngkpl stghryrttc dfgfasldvl tvyaedsgty tckatnklgs aqssikldvk
     6001 srasiiretq hesalkkiqy leddsrykrv dqedliiaek pkfgrplkni ehlpegksah
     6061 leatltpvnd ptmvvewfrd grpipqghkf kttydfgyva ldilyayped sgtymckarn
     6121 avgeavttcv isvdskqglf ldtldaqrlq kireletvev kqevekevvh qkpvfltpln
     6181 nldhlkegeh ahlecrvepi ndpnlkiewf vngvavktgh rfrkthdfgy valdilytyp
     6241 edsgtymcka tnlageavnt ctikvgsrrs illdtqhpdg lekireleaq ghparlevee
     6301 ppvtpprfvt elrgttevye gqtahfecqi eplhdanlri effhngkplp sasrfhvtfd
     6361 fgyvaldigh tvpedageys vrainalgqc vssielrvip rdniildsqr pegldkirel
     6421 eaqqpwkrpd ipepqtrqrp vftqplqnid aipegqtahf ecrlipvgdp tlkvewfrne
     6481 ipletssrit kvhdfgyvsl dinhvreede gvymcrasnp lgeavttasm kikskasiql
     6541 dtqhpeaqrk iaqleadkps rpeepekvfd kpiftqlltg ptelwegqma ryecrvvpvg
     6601 daslrfewyi ngvelkmgsr fhvshdfgyv tldilkvite dsgvytckai nkageavssi
     6661 slkvkarsai dadsiqpdaw qkiqlkeaem nkvpemfvdt tpqqapvftk hlesydklve
     6721 gqhvyleaqv epradpnlrv ewfkngislq tgtrlrstfd fglvtlsing lrpddsaiyt
     6781 ckatnllgea vstcslkivd rhwllgdtlh pdalpkidal eqphvtsaeq pepiyeepvf
     6841 ithlnnvecv egdnvhfecn vepskdptmk iewfingkpl ptgarfksty dfgyvaldit
     6901 hayeedsgvv ivkatnskgs aqtsgtlkct skqsiylqtq hpqgeaglek vkeaedayls
     6961 kyrrpdegpe heypkpiwtv plqpefklge feplhlegqv epkddpnlki ewyfngkvle
     7021 hasrfkmtsd fgfvtldltd vyerdsgiyt ckaynkagea ftsttiycst keniiersqh
     7081 pkgkegleai qdleeslkrq egappeseeg hppvftsqfe nltnlsegei ahfeasltpt
     7141 gdqtmvvewf yngkvleash rirtvyafgm vvlevlgtki edsgtyscra tnkwgqaeis
     7201 velqcvdksk gqkpkftthi qsleglkdgq sahfectlip vgdpqmkvew fhngqplrhs
     7261 srfkmvsdfg fvvmdiagvm ahdtgeyvck asnkygedft katlkcfgks gvyldslqpd
     7321 slarireles fggeqpttpt tpvaeppkfi tqivditrlv egqsahfear ltpvndpdlk
     7381 vewyyngkkl phghryrtfh dfgivildil ycyeensgvy ecrafnkyge dttkatlkcf
     7441 skaslilesq lpkgmeggle kiqtledsmi rtkdekvvee rgkapmftvp lsnidglreg
     7501 esahfearli ptddpklkve wfwngkplrt gsrfrtfcdf gfvileispi ypedsgeysc
     7561 ratneygeav ttctmkctgk rsiilesqlp kgmestidki aeleglgnep gqvtpeddtg
     7621 kppefvttps dltltensla hfecrltpin dssmrvewfh ngkpllagsr iktihdfgfv
     7681 ilevancyqr dsglytckat nrhgeatvnc klqvrgrqgi ilepqlptnf ktgtesiqkl
     7741 eealykrdei lteeetpnpp kftvelkdie veegapshfd crvepvgdst mridwfhngr
     7801 sfatgsrvhq indfgfisld msytyardsg eyvcratnkw gsattkatit ckskktidfd
     7861 sqlpsgmsge klkelergpv seppveeapr qpprfithiq satvdesepv rfecrvepkd
     7921 dpnlriewyr ngkmipaghr yrtmydmgfv smdilyvype dsgeyvckai ndlgedttra
     7981 slsckklpsi ilqnqvpkgm kksealmqme atikkytsev hlteddlyda dkkqpprfvt
     8041 qildqtelve mnsskfecql apvgdpnmkv ewffngkplp hknrftpiyd fgyvamnfgw
     8101 vypedsgeyl cratnlygmd etravirtag kpgiiyesql pkgmksieki remeaawqiv
     8161 peeegeeekv rapptfvskp epvtveegdc srfccrvtgh prprvmwiin ghtvvngsry
     8221 kltydgmyhl dipktrqydh gkveviarss vgesrtettl tvkprsddyr gvlknsprpw
     8281 ydygltqyqt erqntelekv fderhhnvsq gieiatehlg qkvfkepetd wqksvkskkn
     8341 eeyynklmtl eeeqvlketr lresshqfai pgekvvadsv akgmaqkyee tleekkdekt
     8401 qetvqppprp lkkphttevd idvermkvtg kyppgpsest vhgrevhvak qkqtqkevkg
     8461 dleitrkita tettevehka ktqervvqgp tkpakppvft kkiqpcrafe qeqarfevef
     8521 dgdplptvkw yredfpiqns adlqiytfst ksvlivrqvf medsgvfsvi aenrggkakc
     8581 sanlvveerr rqpgrggvip psflstiqnt svttgqlarf dakvtgtkpl dvywlkngkk
     8641 itadiryktl eedntytlll letvpedsgk yecvainkag earcdaectv rgpqspaksa
     8701 kpttpgveka pavleplkdq tiregtsvaf scrisgkpvp tvqwkkgdkv ikpskyfqms
     8761 kegdfctlri seafpedegv ykciaknpag dvttsanlrv lapdaadvlp kltplkdqiv
     8821 segqpaqfkt qvtpskpkpt iqwyregali pqspdfqmih egnnavllia ttyeedtgtf
     8881 tcrattsagt vetsaklivk krtgayyevp aetgpgartg dkpfvslgtg kdvilepipl
     8941 dkgeleiplk pgdetlpwrk grvhhrprla dttpwrktkp ksrdtsldkl qatesqikpw
     9001 teetvtlkkt pqqareipke kleevelkpa kiekkeirrp sletvdlkpf tkettketki
     9061 eekltrreei dkeiyeeeed srflktsrkv detvqrkkde vkpwteekvt lkkskpekke
     9121 lpketletve lkpskigkge vrkpslekvd lkpvpttvte kvsekvtekt eyleqedtsl
     9181 ldvsrteevk kkrkekveek pekpeqakpw teekitlkks kpvvkeieke tletvelkps
     9241 kigkaelrkp slekvdlkpv pttvtekvte kitekteyle qedtslldvs rteevkkmrk
     9301 ekveekpeqa kpwteekitl kkakhlpkei eketvetvel kpskitksei rkssvekvdl
     9361 ktvtketkei tqktdyreee dttllevtkd tqvkktrres ipvekvegpk pwteekvtlk
     9421 kakpekkevp kekpetvelk psrpqkadie kpsledvdlk pipkkvtkek tekdkyieee
     9481 dtsllevtkd tetttkvekk vkrreevekk petkpwtaek itlkkakveq keirresiee
     9541 vtlkptktek rqvtketlek vdlqkvtdkt qfveeedttl mkvdkvekve eervdkmatw
     9601 rrdrkpkeka pyheeedksi ldvkrdeest ekrkevpvpw argkkkptek apyheeedts
     9661 lldvkkdees iekqkevpvp wargkkkpke ekpelvpqqv qpeekpeeee tlektqvpwl
     9721 rdkkvarkip erkeepvedi ksvtlkpvrr erapeekekp eevtlkpvkr ipreeeekee
     9781 vqlkpipkrk peekpqeeii lkpfqkpkpe veeqekpklk pfekakpevp eqekvqlkpf
     9841 krekpevpek ekvelkpfkr ekpeetlvpq kvpekpeeeq pseveevswr rkpkkkeaii
     9901 pekpvekvee vkvvedkvik kqeetiedfk sftlkptkrg kkleekeeke evtlkpvkrl
     9961 pkeeetkaei klkpvekkve ekeakplkpe maptvpeeek ptevsevpwk rgkkkpvekr
    10021 veevtteekk veeeekevse lswrkpqkek vkevkkeqev edftavmlkp trkekkpeek
    10081 eekeeitlkp iqkvqkeeek peavtlkpvk vekpeaveke evpeektelp wrrgkkpakk
    10141 ivpqeevkpe eigkpeervp evkeevpkkp kvekpeeekl evklkpvkrl ekpeeakpee
    10201 vtlkpvkrlp keeeekeevk lkpikrekpe eekpevklkp fkrlekpeee kpeevtlkpv
    10261 krlpkeeeek eevklkpidr kkpeeekpev klkpikrlek peeekpeeva lkpvkrlpke
    10321 eeekeeiklk pikrekpeee kpevklkpfk rlekpeeekp eeitlkpvkr rpkeeeekee
    10381 vklkpikkek peeekpeikl kpikrlekpe eekpeevtlk pvkrlpkeee ekeevklkpi
    10441 kkekpeeekp evklkpfkrl ekpeeekpee atlkpvkrlp keeeekeevk lkpikkekpe
    10501 eekpevklkp vrrlekpeee kpeevtlkpv krlpkeeedk eevklkpikk ekpeeekpev
    10561 klkpvkrlpk eeeekeevkl kpikkekpee ekpevklkpv krlekpeeek peevtlkpvk
    10621 rlpkeeeeke evklkpirke kpeeekpeei klkpvkrldk peeakpeevt lkpvkrlpke
    10681 eeereevklk pvkkekpeee kpeeiklkpv krlekpeetk peevslkpvp kvvpekdeek
    10741 peeeapetsi lpwrrgkktk kvvdfheevt vvpidqkkiv sevheketti veqkveakev
    10801 iekkvvveev kpevveevte vsevswrkpr kpkkpveeke vvelkpvekh eeivekveek
    10861 itlkkvkkev peveekkeiq lkpvpkkpke eevpekitlk pvkkekpeek pkeeeipelk
    10921 pvklekpekv eeteekeevm veitrkkpkk kpeevpveke evveteitei teekpeeisv
    10981 lpwrrpkkvt ekkieveeik pekvedkeve evtettevsw rkqrkpkkpi eeeekpekvt
    11041 lkpvkrekpe ekpkeeipel kpiklekpek veeteekeev kvevtrrrpk kkpeeiqree
    11101 ievetkevvd dvevekiekq eiqiedrkrk rkqrtqvdis iidkdkkiap rfiqklqpvi
    11161 aepestakft ctvfgspvpe iswyrneqel hasekyimtv yettasleit kvkeedagmy
    11221 scrasnpagv atstvnlvif ekeeegiaph fstpikplmv eehkpallec vvtgspvpev
    11281 kwyrgeqelk pkkgreitfn petgeaklhi lepteedeti yrvravnkfg raecranlli
    11341 nnvvkvskpe vlrapkitrp lpalvaergk pltlsadfes kptpevkwfr ngvelapsek
    11401 nviniyenta elfipevtkk dggkyeirvq npvgearssg svtvkdrpdk tdevkaprfi
    11461 eplqpqivae gevvimetkv esyptasfqw fhetrplest pqvrivtqen ksilmikeik
    11521 seyagtytcr aenvggsvtc tatinlletp weetvelvsp tfvkrlspvr vmdgesanlt
    11581 cvvqgkptpk vewyhdskpi kegkeitivq dtegvcslai tevfpedage ytcravnpvg
    11641 eavcttsliv eayeyvpdse iassivatsl ttgqsgseed llspketplf dtdteesape
    11701 iikklpqlip tkdgeltrle vkvkgkpkpe gkwykqgvei vssqefqiee fedgtsvlti
    11761 aetypddtge ivfeahnplg vsttmtylsv egivgtkeyr kpewvthmee mqealratqt
    11821 vprfiqeitd vyvkegdtav fecsysgnpt pdvvwykndk mlmstqnlkv riydddkktv
    11881 ltikhateed dgayvckits etglatskak lhvtdvtdek kfyeeeeeve eqtievkpke
    11941 kpqkkklrka kevkekvkte rvkvdkkevr keklvpkeqp eekkivdiee tqilestdii
    12001 eekptevsqa kkivpiqepv lteataslkk iddekpkdmv pkpeerakrv ieeregivvv
    12061 sevttedmap dftveskvdr aqitseslqt vtvsevhtet svqevrraea kqrkakktvv
    12121 teeeifeerp kevvveekpk kkkktekvkv reevtieeiv erqreniare veeimemlha
    12181 kefgpgeapl relatigylv rqgvsvnein eslyrtenfp alktpeaqna lvqlveregh
    12241 gplitqvlte etttdesvva atvgfrafmr mielqhvtve evlthfapea frprawevte
    12301 ateveteerv tervdvvrkt evhfmerrde rkavrtqdir dikeyedtrq thttlrarkd
    12361 rkpreetked rdegvqvvei eeiedteqek mkkkrkdvee ieeeietviv etdskgrlaq
    12421 kkkkrddlee kedveeeeei iekvrkelkp krpkigrdev eleeieefdv tedksmpsim
    12481 vnvpsqrhqv vpldrtteqa pgkpelekak ltvdtitalt ehivpvqeke isdisrikpi
    12541 erkaslsisp vepysttett vqastgefad tfkptsyeat pgiipsegvq vsetlvsdag
    12601 vtslsvskqe igrtaevsvt lqeattvyet mvrekevpte dfvtpqtvka edtilpqlsl
    12661 svyevheglt edkleplktv atkprvnvta veplvveevr aedkpgkyyp elivptevat
    12721 qsvisqrqrv teemhapeke geyipgrlpp sqvaqigisy gnetavvqqn vvqesegvfi
    12781 sekktetfea ttnvtvlegv svstvqtqdt etsltieetk kavadlnvie mtpavtvetv
    12841 psekekeyqp gdkpdtktae tsissleigs iqstvvqese gvyrpdekpt kalaetsirp
    12901 eehvmiseiq tadypsdfre elkyvqesgt vsvqlteakm iqetlthdre ermeemvkpe
    12961 ermvdtsydv nrsvevfqtt svekeadlrv fempeshrgk tvpthpvvsl evemtqpedn
    13021 lgevkkevps sgtakvehvs lqetivgetv vaenlapvek dkapetkvae iamneveslh
    13081 ttmvvvseke teytqvsdvk gayasteftt qvapmleevr shspteeylp dqkptsgiaq
    13141 pshvplesit vavqelaeke dlyktdvrpd qktanvalte ltsisipevm qvitassaet
    13201 lkketkmkee kgrveveels stiisevisn etedvfttka tpkdqkadfn isgreaaett
    13261 qvtamteaed lavkrpeeqk gkpqvdeltp ltvshvetge aekelptaev pkektaqpni
    13321 fgrdvaetmq vltvtsaeel aapkapegqk gkpqveeltp lsvsqvvsqe aesalpapea
    13381 pterkaqpdm tgrdiaetme vltmtsadil aedvkpteqk gmpgleefvp lsvsqtvste
    13441 kedvlpspea pttktaqpsl tgrdiaetmq vvmmtsekel italapethk gipnveeltp
    13501 ltvsqtvshe aedvfektaa psevtakpaf sgrevaqtse mmivtnvgel ekqvlpdeqk
    13561 gqaqldelmp ltvsqvisge veeglpeaek pkektaqpgi lgrdiaettq vltvtsaeel
    13621 aapkapegqk gkpqveelap ltvsqvvsee aegilpapea pterkaqpdm tgrdiaqtme
    13681 vltmtsvdtf tedkkpdeqk ghpeleefvp lsvtqtvsne medvlpspea pstkiaqpsl
    13741 tgrdvaetmq iltmtseeel itaqtpeqhk gkpnveeltp ltvsqtvsqe aeeilekaaa
    13801 ptevtakpaf sgrevaqtse imivtnvgel ekqvlpdeqk gqpqldeltp ltisqiisge
    13861 veeglpeaek pkektaqpgi lgrdiaettq vltvtsaeel eapkapegqk gkpqveelap
    13921 ltvsqvvsqe aegilpvpea pterkaqadl tgrdvaemme vltmasvdtl tedkkpaeqk
    13981 gvpgleefvp lsvsqtvste keevlpspea pttkvaeasl tgrdiaetmq vvtmtseeel
    14041 isarvpeqhk gifnveelts ltvsqtishe teeilekaaa lgevtakpaf tgrevaqtse
    14101 tlivtnvgel ekqilpeeqk gkprvdemts itvsqvvsge veerlpeaek pkektaqpsm
    14161 lgrdiaettq vltmtsaeel aapkapeeqk gkpqveelap ltvsqvvsqe aegvlpvpea
    14221 pterqaqpdl tgrdvaqitv vmsmasadvm iddkkpeeqk gvpgveefvp lsvsqtvste
    14281 kedvlpspea pttkmaqpsl tgrdiaetmq vvtmtseeel italapethk gkpnveelts
    14341 ltvsqtisqe tevileksaa pseatakpaf tgreiaqtse ilavlnvgel ekhvlpeeqk
    14401 gkpkleeltp ltvsqiisge aeeglptaev pkektaepsm lgrdiaettq vltmasaeel
    14461 mapkapeeqk gkpqveelap ltisqvvsqe aegvlpvpea pterkaqpdl tgrdvaqttv
    14521 vmsmanvdim ieekkpeeqr gipgleefvp lsvsqtvste kedvlpspev pttkmaqpsl
    14581 tghdvaetlq vltmvseeql ttkapeeqkg kpnveeltsl tvsqtvshea edilekavap
    14641 seatakpafl grevaetsqv ltvttvgelq kdvlpeeqkg kpqleelmpl tvsqivsgea
    14701 eerlpeaekp kektaepsml grdiaettqv ltmasaeell apkvpeeqkg kpqveelapl
    14761 tvsqvvsqea egtlpipeap tertaqpdlt grdvaetmev ltmasldtli edkkpaaqtg
    14821 apgleefipl svsqmvstek edvlpspevp tekmaqpsli grdvaetmqv ltmisamelt
    14881 tghapdqqkg vpnveeltsl tvsqtvshet eeilekavap stvtakaals grevaetsqv
    14941 ltvsnveefe ksilpegqkg kprldeltsi tvsqvlsnel eetlpspekp sekiaqpqmi
    15001 grevaeqtev ltvtnveelk klekpegqtg kpdveemsfi tvsevfstem ekempspevp
    15061 kertalpqls sievaettev ltmisaeell kskapeeqkv qpkldeltpl tvshvvseea
    15121 eealptaeip kektaqpsll grdvaetsqv ltmasaeell aakvpeeqkg kpqieelapl
    15181 tvsqvvsqea eealpsaevp vereaqpsmt srdvaqtmlv tsmsnvdima eskipeeqkg
    15241 vpgleefvsl svsqtvstek edvlpspevp tekmaqpsli grdvaettqi ltmisatelt
    15301 tdrapeqqkg vpnveeltsl tvsqavshet eeilekleap gtvtakaals grevaetsqv
    15361 ltvsnveefe ksvlpeeqkg kprldeltsi tvsqvvsnel eetlpspekp sekvaqpqmi
    15421 grevaektev ltvsnveemq klekpegqkg ipdveemsfi tvsevvstea ekelpapeap
    15481 kerkalpqls gievaeksev ltmtmteefa kpkapeeqkg kpqleellsl svsevalgev
    15541 ekglptpdkp teetakpsll giqvaeksqv iassitgelq esikpdtkqi ipeqipfesi
    15601 eqsqtltqer estfvvektt tsataevsfr vseslevtqv tatekeskev ikgvakeasa
    15661 qadvierkva lktevqpedm asefkvkkpe gktakgvdei qqgvivtehl tahefeselp
    15721 esvlpfakla rvsiqadhle hivetteapa qqhhititqh ttkhdlpqqq siesdtevie
    15781 eyttkfrrgs tedataavkt kktivrkkpk ttgeedivvv ieeelddikp kipsipkkqf
    15841 mpieevttvs dveeivsari deaeeivpke eekvevtite vqdtdkkkkv tkkqfvrrrg
    15901 ktrqvteeit veeegkapet tvvtkpveii eedieerpki ekeeyvveev peektiplkp
    15961 rkkltptvqq veeqvtvtee itkegkprkt tkkkvitrkg keqqvtevvt veeqgkapit
    16021 svtegpveev veeiiqklpa pkyakpseve evreqvtvte evtrlgkpkk ttkkkiikrk
    16081 gkeqqvtevv tveeegkapt ttvtegpvee vvieeikelp ipefvkptev eevreqvtvt
    16141 eeltkmgkpk ktikkkiikr kgkeqqvtev vtveeqgkap vttvkegpve evveetitpl
    16201 lapeyakpse veevreqvtv tqevtklgkp kkttkkkiik rkgkeqqvte ivtveeegka
    16261 pkttvtegpv eeiieeeikp efikprevee vreqvtvtqe vtregkpkkt ikkkiikkkg
    16321 keqqvtevvt veeqgkapvt tvtegpveev vdetvkalpa peyakpseve evreqvtvtq
    16381 evtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk ttvtegpvee iieeeikpef
    16441 ikpreveevr eqvtvtqevt regkpkktik kkiikkkgke eqvtevvtve eqgkapvttv
    16501 negpveevvd etvkalpape yaepseveev reqvtvtqev tklgkpkktt kkkiikrkgk
    16561 eqqvteivtv eeegkapktt vtegpveeii eeeikpefik preveevreq vtvtqevtre
    16621 gkpkktikkk iikkkgkeeq vtevvtveeq gkapvttvne gpveevvdet vkalpapeya
    16681 epseveevre qvtvtqevtk lgkpkkttkk kiikrkgkeq qvteivtvee egkapkttvt
    16741 egpveeiiee eikpefikpr eveevreqvt vtqevtregk pkktikkkii kkkgkeqqvt
    16801 elvtveeqgk apvttvtegp veevvdetvk alpapeyakp seveevreqv tvtqevtklg
    16861 kpkkttkkki ikrkgkeqqv teivtveeeg kapkttvteg pveeiieeei kpefikprev
    16921 eevreqvtvt qevtregkpk ktikkkiikk kgkeqqvtev vtveeqgkap vttvtegpve
    16981 evvdetvkal papeyakpse veevreqvtv tqevtklgkp kkttkkkiik rkgkeqqvte
    17041 ivtveeegka pkttvmegpv eevvieeiqe lpipefvkpt eveevreqvt vteevtrlgk
    17101 pkkttkkkii krkgkeqqvt eivtveeqgk apvttvtegp veevvdetik slpapeyakp
    17161 seveevreqv tvtqevtklg kpkkttkkki vkrkgkeqqv teivtveeeg kapkttvteg
    17221 pveevveeev kpllapefvk pseveevheq vtvtqevtre gkpmktikkk iikkkgkaqq
    17281 vtevvtveee gkvpvttvke gpveevvdet vkalpapkya kpseveevre qvtvtqeitk
    17341 lgkpkkttkr kvikrkgkeq qvtevvtvee egkapettvt egpveevlee eikplpgpef
    17401 vkpseveevr eqvtvteevt regkpkktik kkiikkkgke qqvteivtve eqgkapvtsv
    17461 tegpveevvd etikqlpapk yakpseveev reqvtvtqem tklgkpkktt kkkiikrkgk
    17521 eqqvteivtv eeegkapktt mtegpveevv eeeikplpal efvkpsevee vreqvtvtee
    17581 vtregkpkkt ikkkiikkkg keqqitevvt veeqgkapvt tvtkapveei vdetikelpa
    17641 lefakpseve evreqvtvtk evtkegkpkk tikrkvikkk gkeqqvteiv tveeegkapk
    17701 ttitegpvee vveeeikplp alefvkpsev eevreqvtvt eevtregkpk ktikkkiikk
    17761 kgkeqqvtev vtveeqgkap vttvqegpve evidetiksl papeyakpse veeireqvtv
    17821 teeltklgkp kkttkkkitk rkgpeqqite ivtveeegka pvttvtegpv eevvkevlkp
    17881 lpvpkvvkps eveeireqvt vteemtkegk pkkiikkkvi krkgreqtvq evvtveekgk
    17941 apvttvtvgp teeiveetvk tlpapeyakp seveevreqv tvtqevtkeg kpkkttkkkv
    18001 ikrkgkeqqv tevvtveeqg kapvttvteg pveevveell kpliapeyvk pseveevreq
    18061 vtvteeltke gkpkkvikkk vikrrgreqq vkevvtveeq gkapvttvte gpteeiveet
    18121 ikalpapeda kpseveevre qvtvtqevtk egkpkktikk kvtkrkgkeq qvtevvtvee
    18181 kgkapvttvt egpveeivee tvkalpapel vkptevtevp eeltvtevvt kegktkkivk
    18241 kkvpkpkkeg ieapeeapee vpveetkkii klqrlkvskf estkldiqet slgkpqfaqi
    18301 klrktpvakr pskkdekfpr illrsriiai ewppspknlk vdtfepielq ngilsrnvde
    18361 aaalpkpkkk kikriekeia klekldqefe elkkelplek leepileekp erkvkkkkkp
    18421 edkakegvkp qlmrisikeq kpikpkiaep atplfaqikl kkapakpkkk peeevskfpk
    18481 illrsritah ewppsvkypa isvlepnyiq ngilsrtmee alklkkvkhk kmklpekelt
    18541 elekldqefe elkkvplekv eeitpyerkp kpekpeeekp kklvigkgkr rpaeeedlet
    18601 vqlkkipeki psapeeaekv pkkkdevdkp kkekeekeyp elkpfepydi eapeveleer
    18661 ekpeypepek pkekkvpgkk pkkerktpaq eteavpivpg vpkpkepeee eeikrriper
    18721 pppteepeki klkpwkkgek peeeekkped yikfvpkddd ivetvevttt veetpdkkqr
    18781 kikkkktitk pkdekpkiie ettieeegfe pvvtveefdv dkyeavpves vekaapveei
    18841 keeivtseii teegtkktvr kkrvtkkreg kervteevtv eeegkepita tielhedtis
    18901 eiheqakhkr ptkpkadekl aedvtektve qqeiieevke vkptkkvikk ekpvkakdev
    18961 teevveeiii eepekqeakp iveeeetvii tehdvrkapk kkkevvikee eeifetietp
    19021 thkiirkkir pmkkgekevv veeiiqkpre etvemeeqqi vevvetptkk ivkkkkevvk
    19081 ddgvpeelve eiiiekpqkk kakptveeek kkvkvtkvdt kkapekpeev veeeeeiiet
    19141 ietptkkiir kkpkdkkdki ieeviqktie eakameeqei vevietptkk vikkktttie
    19201 egkapeekve eiviekpkev ieeeeeiiet ietptkrivr kkpkdkkdki veeviqktie
    19261 eaktmkeqei ievietptkt vikkkkatik egkapeeeve eiviekpike kakkkilpke
    19321 gveitevite tsveelieee lkpeeakpse eveeekvkit evaikkvpkk kkpskaveae
    19381 ekeevveeik pekpkeekak ptvtekegie vtevisevap eklpeeakpk kkkateiket
    19441 eekvitkkvk vkeapkeavp geeeqpaeev delvpekpkk tkakkevtpq dsivttevvp
    19501 itkeeeleve kpeeekatvv eekeekakve ekkvtvkkap kkklkpteae eeqveevvee
    19561 itpeepkkrk akkavipkee iqttevipgi taeeipeeek peeekavpke eekveeeakv
    19621 tevsvkkker kkkekpvtng evieekpkvk eipeekpkee vpqvpeekek peeekpkevk
    19681 pkekkpkeek pkkpieekpe evkpeeekpe vkpeeekpke ekpkkkkpae kkpkiseeer
    19741 pedkveeipe kkpeevktre eiipkdtvqt tevipetvae eiptkkpker kavpveekee
    19801 kveitevpve kapekkeekv peekpteaap ekpkeakvmk kkkkkpakkd eieewvepey
    19861 erpvlepmpe kivwepkkkk ekkplpeslq klvpqkierk emkptklkys epaetvqfas
    19921 iklkkvavpk kkeadetkfp kimlrsrmtf vgdyppelqi pkityleenp vqtgilsrnt
    19981 eealkvlkrk vkkvklseke vtqlekldle feelkkvple kiedksvyer qpkkpaekpe
    20041 epkkvpigkg kvpeeekvep ekvklkkvpe kkpeeveepl kkpkkpepee evkpekkkkp
    20101 kvklehdelk pldfdrpele kyepeeyekp eepapepvpk pyekepkkkp dqeveqiplk
    20161 kgkpkspepd eepevkfrip tkekpeeepe qitlkpwkke kpeeekekei pkkeeekvpk
    20221 ppkeepedvt vqrkaktkkp kekpkeekee evtvkkpeee kpeevaeevt lkkepekpve
    20281 eapaevtikk pvveekevee eetitevilk kkpekpeevv eeavvkkpkk kkpvkeeevv
    20341 eevtvtkvvt eeekeeekvv ikkpkkksip eiteevteei tlkkpeekap eevteeikkp
    20401 kkkkpvkeev aeeitikkpv veekkeeevp eevtlkpkkp eekpvieevt eeitlkkpvp
    20461 eevteevaik kpkkkkpvee eaadeitlkk pvvvekeeee iteevtlqkk kkpekkpvpe
    20521 eiseeitlkk pvpkeeekap eevteeiaik kpkkkkpvke eaadevtikk pvveekkeee
    20581 tpeevtlkpr kpkekpvpee vkeevtlkkp vpeekekvpe evteeiaikk pkkkkpveee
    20641 aadevtikkp vvvekeeeei teeitlgkkk kpekkpvpee iteeitlkkp vpkeeekape
    20701 evtekievkk pkkkkpveee aadevtikkp vvvekeeeea paeitlkrrp kekpveeite
    20761 evteeitikk ptpeekekap eevsekvevk kpkkkkpvve eaadevtikk vvveekkeee
    20821 apeevtlkpr pkkksvteee vaevtltkpk pieeekkpee seltikkkpk kkpvieeeaa
    20881 evtikkpvpv epdeapeeit irkkkkpekk pkvteeveet avtikkarap ekpteeeeit
    20941 tdvqikkkkp qkkveevaad eltikkieev eqpeeeeevq eftfkrkppk lppkpieeiy
    21001 edvtlrklrp krksrpdike itetenvtfr prttktkedv eqefkislnt yeeedismsg
    21061 kvrlkpkkkp ltyseetgee tiriiqeved dsgpiieeii esdeegkdey sieeldtdem
    21121 rlplrrkkkk vpkpykvedi eegdvklklk rerkysieea deslalklks krrvstydee
    21181 eatlsitree diseeveiey vvrdgdtmfs icsyvaetde ainlvegerv yvidhtnqdw
    21241 wfvkkhltee kgwvpaqyll nevhytlylq rklhekidkl pvfekpapgd kssapkfvek
    21301 lqpihtpdgy tiqfecqveg vprpqitwfr qtaiikpspd fqmyydddnv atliirevfp
    21361 edagtftcva knaagfasst teliveapls dhgsdltgps rkslsressl adilegippt
    21421 fsrkpkakyv degedvilec rlvavpepei twyykdtqit tkeniiiate sdmhmycsvi
    21481 kitkvqkkqe gkytivarnr egeatieipm kvktgksepp eileplqsyv vregetvvls
    21541 tqivgnpapk vtwfkngkpl kgltpkqdgh vntltliqpq vsdtgeysvv atndlgtaet
    21601 katltveinl kpslainfqq htfehfsvtk eipsgapepp lfterfqelt vpekgtfklv
    21661 akvtgnpvpe vtwlrnnkpl ekspnitety dgenivleir nansevdtgd ykcvasnpvg
    21721 kashgarvtv dvdkvtftkt lekevvvdey ktleltcets htvstkwwhn dkeisgmdhr
    21781 eiieegrvhk lvikktnptd egtykctvkn qstssnvtvk atkpefvkkl qdcevkerdv
    21841 aileveitsq tvdvkwykdg eqlgpgkekl efvkegtvrk llirstsvhd egeytcslld
    21901 qectaevtvi elppeiitkm qdvtiarger atfdieltkg dalvrwfkdg qelqfsehvr
    21961 lsidgkrqkl kiydteseda gvyscevgqq kssakltvee pavefitklp dvtlvplnsd
    22021 avfvielsqd vpvtwmrktq vikespkyti idegkvkkli vkkcttedis eytamvanvk
    22081 tssrlrvevi etppkispdt prkykvrkde dvdivvkfsg tpkpedewtv ngrvvtkskr
    22141 aiptideesa vltirkiqee dvgdytlklv nsvgetsiei nvvivqvpsa pgapeplett
    22201 ndsvtlhwkk pdsdgnspiv eyilehqekt ettwtkitet itetthkvtk lttnkeytfr
    22261 vtavneagpg etsptspyvk iskpstaepp vileplksiv iglgetvtls cviggtpape
    22321 itwlrndeif edtsityenh itkytirett etssakftvk aknnvgsaet tcelkiqesp
    22381 kiiydenltn qklpmngqwk ieiqitgfpk pevtwtkngk evdekrvsih reertstvsi
    22441 sslvredtat ymakaenqag sssvdlhlkv idkpskpqgp vvfkeirqdr vtlewrppad
    22501 dggielekyt iekcepgktw vkvvdidkev esfcvhklqq naeykfriia rnavgasepl
    22561 esdtvkmrts feppgaprgp levsgmtkts ftikwqppen dggtpitdyi ieikeeskkt
    22621 wqkigstkhe tthfsvsdlk tdvpynfrit aknsvgsgpp yvaeepiapg rrikitklte
    22681 ssmyallgif pptkiviskt ppsspqhvhv tnitsksatl swsppastgg teltgyviek
    22741 rpligkgarw tkvvtldatt lqycvenlke seflfrifae nnqglsvpan sepvtlktha
    22801 svpspptapl eirqiaantv viswgrpdsd ggaplegykv airdtkrtmw mevarvntei
    22861 qklnirdlqe nhmylirifa rneiglsdpl eseepfkiip aseltvvepi aeatekgeta
    22921 sisfstents swlrehnmda dihsyararl lrqdeyffri whyakklfe
//
LOCUS       XP_076546012           22969 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X12 [Osmia lignaria lignaria].
ACCESSION   XP_076546012
VERSION     XP_076546012.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689897.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..22969
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..22969
                     /product="sallimus isoform X12"
                     /calculated_mol_wt=2595687
     Region          78..277
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            156..161
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <458..1112
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <705..1015
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1284..1373
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1301..1305
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1314..1318
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1339..1343
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1353..1358
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1366..1369
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1385..1474
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1402..1406
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1415..1419
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1440..1444
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1454..1459
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1467..1470
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1484..1573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1501..1505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1514..1518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1539..1543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1553..1558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1566..1569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1596..1685
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1613..1617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1626..1630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1651..1655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1665..1670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1678..1681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1711..1801
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1728..1732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1741..1745
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1767..1771
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1781..1786
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1794..1797
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1823..1911
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1840..1844
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1853..1857
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1877..1881
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1891..1896
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1904..1907
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2079..2168
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2096..2100
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2109..2113
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2131..2138
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2148..2153
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2161..2164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2175..2265
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2192..2196
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2205..2209
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2231..2235
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2245..2250
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2258..2261
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <2900..3173
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          3886..3975
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3903..3907
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3916..3920
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3941..3945
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3955..3960
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3968..3971
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4132..4219
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4149..4153
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4162..4166
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4189..4193
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4203..4208
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4329..4418
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4346..4350
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4359..4363
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4384..4388
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4398..4403
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4411..4414
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4454..4544
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4471..4475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4484..4488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4510..4514
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4524..4529
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4537..4540
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4553..4643
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4570..4574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4583..4587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4609..4613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4623..4628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4636..4639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4700..4791
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4717..4721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4732..4736
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4758..4761
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4771..4776
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4784..4787
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4833..4925
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4851..4855
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4866..4870
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4891..4895
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4905..4910
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4918..4921
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4972..5064
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4990..4994
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5005..5009
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5030..5034
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5044..5049
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5057..5060
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5106..5198
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5124..5128
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5139..5143
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5165..5169
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5178..5183
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5191..5194
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5240..5332
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5258..5262
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5273..5277
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5298..5302
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5312..5317
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5325..5328
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5374..5465
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5391..5395
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5406..5410
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5432..5435
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5445..5450
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5461
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5507..5597
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5525..5529
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5540..5544
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5565..5569
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5579..5584
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5592..5595
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5641..5732
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5658..5662
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5669..5677
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5698..5702
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5712..5717
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5725..5728
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5774..5865
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5791..5795
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5806..5810
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5845..5850
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5858..5861
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5907..5999
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5925..5929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5940..5944
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5979..5984
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5992..5995
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6041..6133
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6059..6063
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6072..6078
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6099..6103
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6113..6118
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6126..6129
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6173..6265
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6191..6195
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6204..6210
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6231..6235
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6245..6250
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6258..6261
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6306..6398
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6324..6328
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6337..6343
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6364..6368
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6378..6383
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6391..6394
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6440..6532
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6458..6462
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6498..6502
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6512..6517
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6525..6528
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6572..6664
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6605..6609
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6630..6634
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6644..6649
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6706..6798
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6724..6728
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6739..6743
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6764..6768
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6778..6783
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6791..6794
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6838..6928
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6855..6859
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6870..6874
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6895..6899
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6909..6914
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6922..6925
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7005..7065
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7009..7013
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7034..7038
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7048..7053
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7062..7065
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7113..7204
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7146..7150
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7175
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7185..7190
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7214..7305
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7232..7236
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7247..7251
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7269..7276
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7286..7291
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7299..7302
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7347..7438
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7365..7369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7380..7384
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7402..7406
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7419..7424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7432..7435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7485..7576
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7518..7522
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7543..7547
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7557..7562
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7623..7714
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7640..7644
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7655..7659
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7680..7684
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7694..7699
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7707..7710
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7760..7850
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7777..7781
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7792..7796
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7817..7821
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7831..7836
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7844..7847
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7893..7982
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7910..7914
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7925..7929
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7950..7954
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7964..7969
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7977..7980
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8036..8126
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8054..8058
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8069..8073
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8091..8095
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8108..8113
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8121..8124
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8174..8262
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8191..8195
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8204..8208
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8228..8232
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8242..8247
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8255..8258
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8497..8587
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8514..8518
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8527..8531
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8552..8556
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8566..8571
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8579..8582
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8601..8690
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8618..8622
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8631..8635
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8660
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8670..8675
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8683..8686
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8711..8800
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8728..8732
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8741..8745
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8766..8770
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8780..8785
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8796
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8812..8899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8826..8830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8840..8844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8865..8869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8879..8884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8892..8895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <9016..9669
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <9722..9875
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          11150..11239
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11167..11171
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11180..11184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11205..11209
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11219..11224
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11249..11340
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11266..11270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11279..11283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11306..11310
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11320..11325
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11333..11336
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11355..11444
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11372..11376
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11385..11389
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11410..11414
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11424..11429
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11439..11442
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11457..11543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11474..11478
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11487..11491
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11512..11516
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11526..11531
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11539..11542
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11560..11650
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11577..11581
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11590..11594
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11616..11620
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11630..11635
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11643..11646
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          11698..11784
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          11717..11721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11730..11734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11756..11760
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11770..11775
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11822..11913
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          11839..11843
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          11852..11856
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          11879..11883
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          11893..11898
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          11906..11909
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <15401..>15553
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          21207..21259
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(21212,21214,21220..21221,21238..21240,21253,21255,
                     21257..21258)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          21295..21385
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21312..21316
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21325..21329
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21351..21355
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21365..21370
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21378..21381
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21419..21512
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21436..21440
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21449..21453
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21474..21482
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21492..21497
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21505..21508
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21520..21606
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21537..21541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21550..21554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21572..21576
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21586..21591
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21599..21602
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21640..21730
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21657..21661
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21670..21674
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21695..21699
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21710..21715
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21723..21726
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21743..21820
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          21753..21757
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21764..21768
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21789..21793
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21803..21808
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21824..21909
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21841..21845
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21853..21857
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21879..21883
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21893..21898
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21902..21905
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21914..21998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21931..21935
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21943..21947
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21968..21972
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21982..21987
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21991..21994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22104..22184
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22112..22116
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22125..22129
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22150..22154
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22164..22169
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22177..22180
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22188..22279
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22188,22253,22268)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(22269..22270,22272..22273)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          22290..22376
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          22307..22311
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22320..22324
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22342..22346
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22356..22361
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22369..22372
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22400..22470
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          22400..22403
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          22412..22416
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          22436..22440
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          22450..22455
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          22463..22466
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          22474..22566
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(22474,22540,22555)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <22544..22859
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(22556..22557,22559..22560)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..22969
                     /gene="sls"
                     /coded_by="XM_076689897.1:60..68969"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mvdghdsmet tensegcrek esrtvvesne dapqqttspt ttistiavqs getrlviall
       61 qsgewlrlkv levqpeltkl gasveeatel ssahdevllr lqskqspvee llrqadqlis
      121 nqrpraevya amaetlgqaw rdvnellerr kqildcnvlf qcraeecres mralemacnd
      181 tllpieieav knflskihdl rknmlealmg alqegknlld rlkeianegt ldsrpdrikl
      241 eadhavlkve kwleelhdrr rlieasfrsr ktqleqclal allatdlrdl eeilndriaa
      301 lssscdqlgd sassaelllf elkklqaeak efqdrsikit ksterlvssg hfageqateq
      361 ayailgaaad yvndldqyes llnravaffd sarsaltkld qleiqlvtte hpqystrlar
      421 fhaqtvatie dvtakplaeg yalldvtgrg apgaegvkrt vedlenrkir lmerctahek
      481 enleisrmin tflekhdelr kwlmsipeaf lqghqdmgsd vpmaedfcrl hrqlsndler
      541 rtdevehlef eilpirekle esqklelksk veelqrswsk tkelvanrid lgsvylqfhm
      601 vadeltreve sieselksna dvlderkvee lgrrwkdlqp lyvkltgsgk afldaaikid
      661 dpyldvprac lcvqtllekf anrqltvtes wekwrtsiei lrerrieher kieestrtle
      721 wvskfseqly pvitsqsaet tmilkdlags kqrilpelnk avseldsrik gintlaqkge
      781 vhvdeeilkr lhqmhedlrk tardyeille slisifesie eveqkietlk iqvervtslk
      841 kifeveaafs eidatkwsis drlghiklri edaivrikqq eppgagvkdi eklrraldsl
      901 tsdfeifwsq tttkveehrr tctfiedler idgelknlne hlknvdarmg enlqtakats
      961 asfvqfektv tileqriekf vqtteesiki ltpqivddis llkerwrnlk krmeetkkrm
     1021 nlcidyfvll eeakewnreg skllvviark atmvkapkda tdllheieny lkpgeevqer
     1081 rieklkelst ivfgterlpq fnevivenrq mldsftvvss elrtlvqnlk naedlqeklr
     1141 iekqeadekl qaakvemaaa aaareeaena rkiaeklaae tlekaatevk tlkeereaqt
     1201 msappsfsvs aqtekiektd esyvqetvts avttkeihil qkteiektvp liqrepspgk
     1261 iivteeihek sekiekddvp plapeftipl ndatvqegek fsfqcnlvgh plpevvwykd
     1321 gisilnnpdy lttyvrdvct ltieetfaed sakytcrafn iagsaetsat ltvketapee
     1381 qpsppvfvke lvpstaiegs shqidcvveg nplptvqwfk ndvnidnspd yvitynngea
     1441 vlkfeevlpe dkaiymckat nrlgqastsa slnvqrtect tekpqfvtpl snamgragqr
     1501 vkleceargk pmptltwyhd grsveetmnl kiqtdggrss liiseafakd agcymvvakn
     1561 dageatvscn vsvkgrlphe tsdsefacsd mepvvpkiqm plkdlrvqeg rsvrldcviv
     1621 gqpepeviwy hddqpvkesa dfqllfqgdk ctlliheafl ddaglykvva insggeassq
     1681 ctltvtpvsv thelektrte veetvtagsp pkfvklptds liaegenaif ecavvgepkp
     1741 elkwfsdsge iseseririq rkedgtsilr ilsaipedkg nyvvkafnah geakafarlv
     1801 vrslgdfrrk eefvqmeekl iaptfkerfe drkvpdgvst kfecivvgkp spkiqwlfnd
     1861 rpvhgkdflv svsgdrqvlt ipetgsthvg tiscvaenaa gkatctarle iggsevqrdk
     1921 kevveekvye lveripgdem hassssdkhf saekseigge nkvltkmset itesstthkt
     1981 lkkeyisssm ssstgapgqe ptsvcvkstv hsteqsssad gappvvqsyk veehekiiqd
     2041 qpgeirqert vvvsqdeegi krdikatqip kpvrkstapr fvspvtgmiv dqgsdivleg
     2101 iidgfpqpvv iwskngqelk tkdsmtitys hnhvrlelkn vnvkdagryt ctasnevgsa
     2161 sstadlvvkk tifppvfgrr lqaqlvkrgd rvnmeveitg tpeptvswfk ddipikerpp
     2221 elrikqqgnc ymlimekaak ehagkymvra tnaggeaqsi adfavfeptp dtmvevhktv
     2281 iydvqdkklm qsdekkaevp sskhltseqv sttkiqpaat ikttptamst smaqmsrtve
     2341 kikepdtelc rsekisstve shqmerkseq kyhmklehkm psivepkteg ktvikevire
     2401 evrssepkst lieekienen ietsriakkd alsffesmtr etetipkgpk emiklvddad
     2461 ghevkvgklt knyekstsfq evkkpevkpp ellttkkavh eiftkleqgs ipsrgidnkl
     2521 fdfpyepykp tmetkktvve etissgsssl qssmtmskme skreskmesk ssellsegfn
     2581 lvpepppeig ympkpeevkk krpevsikak qlqesfdktl spidapiggv kifpspspki
     2641 letpkvapps fsmppppfql ekkeiveevc ikkdvhekrd pqplvrpisp srpwssssdv
     2701 etrsqmstdl seyrchsaas shqeilrsss prpsadglam ekswakkcad strkswppqe
     2761 dtpikykqew qmpgedykts skefkrevee tpeggikkts messstterr swsskqetmd
     2821 kmskrapspp kiqpiiynae tikvdhtvnk ieekslyeky isecdvkksq ksekleeyss
     2881 kkwtgaedlk apglvrslet pkkptpplyr pvepiptdqi ilepgpppei gyipapvvre
     2941 kkieqiektl evslehkpak ippggirtip papkkeeppa lppkdvritp pplpakqptp
     3001 veplepfpfk pspsptiktp kvavpaptps kfvkgsfgve sdyesdmdsh mkakwrpyes
     3061 dneepryrrv qppvpkqprp rstepeplpp skfevpsest glprpvltke qqekmykktt
     3121 tfkrqeketk qqkyqvvqpp pvelkpgspp iyvqpatksp tpksppakkp espkfkvktf
     3181 qqesgymadt deplqqkssv aslsksfgkq essssmsshm esrtsysesr seyfesksyd
     3241 srqqqqkkes fapsapssvk qppqqrssfv eksygssavt ssprfmsake tlyttdqshk
     3301 kfeapkkilp pspspsrfvk sefresdyes dydgripplw kprgyesddq sfrtvkpnla
     3361 gpgkpkvqre dtsrlieeas traqkaktfe kreervatkp ktvllpgspp eiayappqpq
     3421 ptyyearsgi pfhnaigtet kktvrmdest ensrrivtve qtsrvikfgd nqksatdfds
     3481 fdsssaqkqk thykvptpkk fvqgqfresd yesdidtrik pkwappesds eepryrkvqp
     3541 pttkttrsss apvrqehvvs pmefdtgptv lrkqtsmsql rderkfdgqv krqassrqee
     3601 tlkpgsppef gyisrsdvkk aanhvasrhm sdmtssfksk tekfvtdiqs dlkqgkpilk
     3661 qpvdgrasda deprtyrees rlsehgtkqi dpdtgliyfk ydfgyefgiv lpgegkktvs
     3721 stsrsvqgqr rasdievpiv hefttrkeng fakrsassss tarqgkpagk fsgsktvkwe
     3781 ptseseyses edirnarkrh seggntmtap slvipcspsp srwdhttpsp lslspslpsl
     3841 spryssatgp psnvdsagsp wpttngvass keviqpylei lpkkaplfit plrdiavvsg
     3901 qtarfecivq aepqpnilws kdgriienst syeihyrngv crltivrafp edagtyacta
     3961 tnslgstvts atllvpgvps pfygtyrdqn lsersaespp kyvalrdlfl pqesdvktas
     4021 snrqqqhnss tfeyawsvnr syaggryrek enateecvei tsrnpiarrl eysdfqands
     4081 frdvadfcfd reystcnpsi asprntvqge ivmqttptvp aftgrpgdps ppvfeqifkn
     4141 arfaqggnai fegrvrgnpk ptvtwthkga plleswkirm syddktgavt lqinqigpgd
     4201 egeytcsakn qygeaicsvy iqpegfgppp qqqiggyrke yaqtfqsteq ksqtgsqsfq
     4261 qrsyqqtidk rsyvngtsts iedfkvdtfe yrllretefr esitrrfvge sdvqistvvd
     4321 rslgpvappq isqkprnskl iegsdavfta kisgnpkprl twfkngqrir esqrvemshs
     4381 nqqatlrirv alpedsghyt llsenpqgct vssaylaies sdqvdqayqt presvktpqv
     4441 estgesdsgk vlppnfvrtc tdrdategkm trfdcrvtgr pypevtwyin gqqvandlth
     4501 kilvnesgnn slmitnvsra dagvvtcvar nkagetsfqc nlnviekeqv vapkfverft
     4561 ttnvkegepv vfmaravgtp vpritwqkdg vpitpgpdvr istdgsgast ldipcaklsd
     4621 aawyqctaqn vagstatrar lfvetpkgaa peprrlnlpr ptkviepepa pgpeviylrh
     4681 verakpylpp peedrvyppp rfiiplrdvh qieggrihfe ariepvgdpt mrvewyvngr
     4741 aldassrats ifrfgfisld lisiilqdsg eyvcrvvsst gvaesratls vtpratieqa
     4801 sqhpdslqyi qqledyskyq rqesveeiss qrpafirplq dlgelqegrn ahfeaqltpv
     4861 sdptmkvewy kdgrpitass rittifnfgy vslnilhlra edagsytvra vnrlgeavss
     4921 aslrvyarts vttdlgipeq qryieaaeel eayqqamhqk yvqeqpepts ppefkspikd
     4981 qnsireggfa hfearlepvg dsdlrvewlk dgrpveassr ittffnfgyv altikyvtih
     5041 dvgiytcray nrageahtta qlsviskndv iydsqhptgl qkiqtledss rysrqvqeet
     5101 qvtqaprflg tlkgtnkive gqrahfearv epqsdltmti ewyhngkait aanriqtyhd
     5161 fgyvaidilq vrpedagtyt vvarnalgea rlsatmvvet rssvdtssmh rtsyektqrl
     5221 eeskfvepqy hieeiskskp ifvqplsdpk pvsegknihl ecrlepmgdp tmrvewfqng
     5281 rpvtvgsrfr tyydfgfval divhstilds geytvratnh lgtahtsacv rvigksdivt
     5341 etqheqsleq iqmledaary rktqqeevtv mqapqftrpl hnietveltn vhlecrlqpv
     5401 gdatmkvewf vngrpvktgh rfrpsyefdy valdilgvyp edsgvytcqa rnqlgeavts
     5461 csvrvhakkd lllesqhpeg leriqyleda srykrhelvd evvkvkprfv tapknqenlh
     5521 egqhahfeck lepvtdsnlk vewykngrpv tighrfrpih dfgyvaldvi dliaedsgty
     5581 tcravnlvgs devscslscr staqvltdtk neqgleqihy ledrskyqrr edveetttqa
     5641 pifttslnnv eikegqrahf ecrlipvsda tmkvewfhnn kpvkagsrfi etnsfgfval
     5701 dimyaypeds gtytcrakni igeaitsata nvhskksiyt etqneetlqr lhhledtsry
     5761 qrkttteeiv tqapvftmpi kdlkvaenqa ahfearvipv gdsklkvewl rngvpiaasn
     5821 rvttmhdfgy valnmkyvnp edsgtytcra vndlgeavts atlfvqskaa lqfesqhesa
     5881 lsklqaledt sryqrheeee ivvkekpsft vqlngptnlv egqsahyecr iepypdpnmk
     5941 vewfhngkpl stghryrttc dfgfasldvl tvyaedsgty tckatnklgs aqssikldvk
     6001 srasiiretq hesalkkiqy leddsrykrv dqedliiaek pkfgrplkni ehlpegksah
     6061 leatltpvnd ptmvvewfrd grpipqghkf kttydfgyva ldilyayped sgtymckarn
     6121 avgeavttcv isvdskqglf ldtldaqrlq kireletvev kqevekevvh qkpvfltpln
     6181 nldhlkegeh ahlecrvepi ndpnlkiewf vngvavktgh rfrkthdfgy valdilytyp
     6241 edsgtymcka tnlageavnt ctikvgsrrs illdtqhpdg lekireleaq ghparlevee
     6301 ppvtpprfvt elrgttevye gqtahfecqi eplhdanlri effhngkplp sasrfhvtfd
     6361 fgyvaldigh tvpedageys vrainalgqc vssielrvip rdniildsqr pegldkirel
     6421 eaqqpwkrpd ipepqtrqrp vftqplqnid aipegqtahf ecrlipvgdp tlkvewfrne
     6481 ipletssrit kvhdfgyvsl dinhvreede gvymcrasnp lgeavttasm kikskasiql
     6541 dtqhpeaqrk iaqleadkps rpeepekvfd kpiftqlltg ptelwegqma ryecrvvpvg
     6601 daslrfewyi ngvelkmgsr fhvshdfgyv tldilkvite dsgvytckai nkageavssi
     6661 slkvkarsai dadsiqpdaw qkiqlkeaem nkvpemfvdt tpqqapvftk hlesydklve
     6721 gqhvyleaqv epradpnlrv ewfkngislq tgtrlrstfd fglvtlsing lrpddsaiyt
     6781 ckatnllgea vstcslkivd rhwllgdtlh pdalpkidal eqphvtsaeq pepiyeepvf
     6841 ithlnnvecv egdnvhfecn vepskdptmk iewfingkpl ptgarfksty dfgyvaldit
     6901 hayeedsgvv ivkatnskgs aqtsgtlkct skqsiylqtq hpqgeaglek vkeaedayls
     6961 kyrrpdegpe heypkpiwtv plqpefklge feplhlegqv epkddpnlki ewyfngkvle
     7021 hasrfkmtsd fgfvtldltd vyerdsgiyt ckaynkagea ftsttiycst keniiersqh
     7081 pkgkegleai qdleeslkrq egappeseeg hppvftsqfe nltnlsegei ahfeasltpt
     7141 gdqtmvvewf yngkvleash rirtvyafgm vvlevlgtki edsgtyscra tnkwgqaeis
     7201 velqcvdksk gqkpkftthi qsleglkdgq sahfectlip vgdpqmkvew fhngqplrhs
     7261 srfkmvsdfg fvvmdiagvm ahdtgeyvck asnkygedft katlkcfgks gvyldslqpd
     7321 slarireles fggeqpttpt tpvaeppkfi tqivditrlv egqsahfear ltpvndpdlk
     7381 vewyyngkkl phghryrtfh dfgivildil ycyeensgvy ecrafnkyge dttkatlkcf
     7441 skaslilesq lpkgmeggle kiqtledsmi rtkdekvvee rgkapmftvp lsnidglreg
     7501 esahfearli ptddpklkve wfwngkplrt gsrfrtfcdf gfvileispi ypedsgeysc
     7561 ratneygeav ttctmkctgk rsiilesqlp kgmestidki aeleglgnep gqvtpeddtg
     7621 kppefvttps dltltensla hfecrltpin dssmrvewfh ngkpllagsr iktihdfgfv
     7681 ilevancyqr dsglytckat nrhgeatvnc klqvrgrqgi ilepqlptnf ktgtesiqkl
     7741 eealykrdei lteeetpnpp kftvelkdie veegapshfd crvepvgdst mridwfhngr
     7801 sfatgsrvhq indfgfisld msytyardsg eyvcratnkw gsattkatit ckskktidfd
     7861 sqlpsgmsge klkelergpv seppveeapr qpprfithiq satvdesepv rfecrvepkd
     7921 dpnlriewyr ngkmipaghr yrtmydmgfv smdilyvype dsgeyvckai ndlgedttra
     7981 slsckklpsi ilqnqvpkgm kksealmqme atikkytsev hlteddlyda dkkqpprfvt
     8041 qildqtelve mnsskfecql apvgdpnmkv ewffngkplp hknrftpiyd fgyvamnfgw
     8101 vypedsgeyl cratnlygmd etravirtag kpgiiyesql pkgmksieki remeaawqiv
     8161 peeegeeekv rapptfvskp epvtveegdc srfccrvtgh prprvmwiin ghtvvngsry
     8221 kltydgmyhl dipktrqydh gkveviarss vgesrtettl tvkprsddyr gvlknsprpw
     8281 ydygltqyqt erqntelekv fderhhnvsq gieiatehlg qkvfkepetd wqksvkskkn
     8341 eeyynklmtl eeeqvlketr lresshqfai pgekvvadsv akgmaqkyee tleekkdekt
     8401 qetvqppprp lkkphttevd idvermkvtg kyppgpsest vhgrevhvak qkqtqkevkg
     8461 dleitrkita tettevehka ktqervvqgp tkpakppvft kkiqpcrafe qeqarfevef
     8521 dgdplptvkw yredfpiqns adlqiytfst ksvlivrqvf medsgvfsvi aenrggkakc
     8581 sanlvveerr rqpgrggvip psflstiqnt svttgqlarf dakvtgtkpl dvywlkngkk
     8641 itadiryktl eedntytlll letvpedsgk yecvainkag earcdaectv rgpqspaksa
     8701 kpttpgveka pavleplkdq tiregtsvaf scrisgkpvp tvqwkkgdkv ikpskyfqms
     8761 kegdfctlri seafpedegv ykciaknpag dvttsanlrv lapdaadvlp kltplkdqiv
     8821 segqpaqfkt qvtpskpkpt iqwyregali pqspdfqmih egnnavllia ttyeedtgtf
     8881 tcrattsagt vetsaklivk krtgayyevp aetgpgartg dkpfvslgtg kdvilepipl
     8941 dkgeleiplk pgdetlpwrk grvhhrprla dttpwrktkp ksrdtsldkl qatesqikpw
     9001 teetvtlkkt pqqareipke kleevelkpa kiekkeirrp sletvdlkpf tkettketki
     9061 eekltrreei dkeiyeeeed srflktsrkv detvqrkkde vkpwteekvt lkkskpekke
     9121 lpketletve lkpskigkge vrkpslekvd lkpvpttvte kvsekvtekt eyleqedtsl
     9181 ldvsrteevk kkrkekveek pekpeqakpw teekitlkks kpvvkeieke tletvelkps
     9241 kigkaelrkp slekvdlkpv pttvtekvte kitekteyle qedtslldvs rteevkkmrk
     9301 ekveekpeqa kpwteekitl kkakhlpkei eketvetvel kpskitksei rkssvekvdl
     9361 ktvtketkei tqktdyreee dttllevtkd tqvkktrres ipvekvegpk pwteekvtlk
     9421 kakpekkevp kekpetvelk psrpqkadie kpsledvdlk pipkkvtkek tekdkyieee
     9481 dtsllevtkd tetttkvekk vkrreevekk petkpwtaek itlkkakveq keirresiee
     9541 vtlkptktek rqvtketlek vdlqkvtdkt qfveeedttl mkvdkvekve eervdkmatw
     9601 rrdrkpkeka pyheeedksi ldvkrdeest ekrkevpvpw argkkkptek apyheeedts
     9661 lldvkkdees iekqkevpvp wargkkkpke ekpelvpqqv qpeekpeeee tlektqvpwl
     9721 rdkkvarkip erkeepvedi ksvtlkpvrr erapeekekp eevtlkpvkr ipreeeekee
     9781 vqlkpipkrk peekpqeeii lkpfqkpkpe veeqekpklk pfekakpevp eqekvqlkpf
     9841 krekpevpek ekvelkpfkr ekpeetlvpq kvpekpeeeq pseveevswr rkpkkkeaii
     9901 pekpvekvee vkvvedkvik kqeetiedfk sftlkptkrg kkleekeeke evtlkpvkrl
     9961 pkeeetkaei klkpvekkve ekeakplkpe maptvpeeek ptevsevpwk rgkkkpvekr
    10021 veevtteekk veeeekevse lswrkpqkek vkevkkeqev edftavmlkp trkekkpeek
    10081 eekeeitlkp iqkvqkeeek peavtlkpvk vekpeaveke evpeektelp wrrgkkpakk
    10141 ivpqeevkpe eigkpeervp evkeevpkkp kvekpeeekl evklkpvkrl ekpeeakpee
    10201 vtlkpvkrlp keeeekeevk lkpikrekpe eekpevklkp fkrlekpeee kpeevtlkpv
    10261 krlpkeeeek eevklkpidr kkpeeekpev klkpikrlek peeekpeeva lkpvkrlpke
    10321 eeekeeiklk pikrekpeee kpevklkpfk rlekpeeekp eeitlkpvkr rpkeeeekee
    10381 vklkpikkek peeekpeikl kpikrlekpe eekpeevtlk pvkrlpkeee ekeevklkpi
    10441 kkekpeeekp evklkpfkrl ekpeeekpee atlkpvkrlp keeeekeevk lkpikkekpe
    10501 eekpevklkp vrrlekpeee kpeevtlkpv krlpkeeedk eevklkpikk ekpeeekpev
    10561 klkpvkrlpk eeeekeevkl kpikkekpee ekpevklkpv krlekpeeek peevtlkpvk
    10621 rlpkeeeeke evklkpirke kpeeekpeei klkpvkrldk peeakpeevt lkpvkrlpke
    10681 eeereevklk pvkkekpeee kpeeiklkpv krlekpeetk peevslkpvp kvvpekdeek
    10741 peeeapetsi lpwrrgkktk kvvdfheevt vvpidqkkiv sevheketti veqkveakev
    10801 iekkvvveev kpevveevte vsevswrkpr kpkkpveeke vvelkpvekh eeivekveek
    10861 itlkkvkkev peveekkeiq lkpvpkkpke eevpekitlk pvkkekpeek pkeeeipelk
    10921 pvklekpekv eeteekeevm veitrkkpkk kpeevpveke evveteitei teekpeeisv
    10981 lpwrrpkkvt ekkieveeik pekvedkeve evtettevsw rkqrkpkkpi eeeekpekvt
    11041 lkpvkrekpe ekpkeeipel kpiklekpek veeteekeev kvevtrrrpk kkpeeiqree
    11101 ievetkevvd dvevekiekq eiqiedrkrk rkqrtqvdis iidkdkkiap rfiqklqpvi
    11161 aepestakft ctvfgspvpe iswyrneqel hasekyimtv yettasleit kvkeedagmy
    11221 scrasnpagv atstvnlvif ekeeegiaph fstpikplmv eehkpallec vvtgspvpev
    11281 kwyrgeqelk pkkgreitfn petgeaklhi lepteedeti yrvravnkfg raecranlli
    11341 nnvvkvskpe vlrapkitrp lpalvaergk pltlsadfes kptpevkwfr ngvelapsek
    11401 nviniyenta elfipevtkk dggkyeirvq npvgearssg svtvkdrpdk tdevkaprfi
    11461 eplqpqivae gevvimetkv esyptasfqw fhetrplest pqvrivtqen ksilmikeik
    11521 seyagtytcr aenvggsvtc tatinlletp weetvelvsp tfvkrlspvr vmdgesanlt
    11581 cvvqgkptpk vewyhdskpi kegkeitivq dtegvcslai tevfpedage ytcravnpvg
    11641 eavcttsliv eayeyvpdse iassivatsl ttgqsgseed llspketplf dtdteesape
    11701 iikklpqlip tkdgeltrle vkvkgkpkpe gkwykqgvei vssqefqiee fedgtsvlti
    11761 aetypddtge ivfeahnplg vsttmtylsv egivgtkeyr kpewvthmee mqealratqt
    11821 vprfiqeitd vyvkegdtav fecsysgnpt pdvvwykndk mlmstqnlkv riydddkktv
    11881 ltikhateed dgayvckits etglatskak lhvtdvtdek kfyeeeeeve eqtievkpke
    11941 kpqkkklrka kevkekvkte rvkvdkkevr keklvpkeqp eekkivdiee tqilestdii
    12001 eekptevsqa kkivpiqepv lteataslkk iddekpkdmv pkpeerakrv ieeregivvv
    12061 sevttedmap dftveskvdr aqitseslqt vtvsevhtet svqevrraea kqrkakktvv
    12121 teeeifeerp kevvveekpk kkkktekvkv reevtieeiv erqreniare veeimemlha
    12181 kefgpgeapl relatigylv rqgvsvnein eslyrtenfp alktpeaqna lvqlveregh
    12241 gplitqvlte etttdesvva atvgfrafmr mielqhvtve evlthfapea frprawevte
    12301 ateveteerv tervdvvrkt evhfmerrde rkavrtqdir dikeyedtrq thttlrarkd
    12361 rkpreetked rdegvqvvei eeiedteqek mkkkrkdvee ieeeietviv etdskgrlaq
    12421 kkkkrddlee kedveeeeei iekvrkelkp krpkigrdev eleeieefdv tedksmpsim
    12481 vnvpsqrhqv vpldrtteqa pgkpelekak ltvdtitalt ehivpvqeke isdisrikpi
    12541 erkaslsisp vepysttett vqastgefad tfkptsyeat pgiipsegvq vsetlvsdag
    12601 vtslsvskqe igrtaevsvt lqeattvyet mvrekevpte dfvtpqtvka edtilpqlsl
    12661 svyevheglt edkleplktv atkprvnvta veplvveevr aedkpgkyyp elivptevat
    12721 qsvisqrqrv teemhapeke geyipgrlpp sqvaqigisy gnetavvqqn vvqesegvfi
    12781 sekktetfea ttnvtvlegv svstvqtqdt etsltieetk kavadlnvie mtpavtvetv
    12841 psekekeyqp gdkpdtktae tsissleigs iqstvvqese gvyrpdekpt kalaetsirp
    12901 eehvmiseiq tadypsdfre elkyvqesgt vsvqlteakm iqetlthdre ermeemvkpe
    12961 ermvdtsydv nrsvevfqtt svekeadlrv fempeshrgk tvpthpvvsl evemtqpedn
    13021 lgevkkevps sgtakvehvs lqetivgetv vaenlapvek dkapetkvae iamneveslh
    13081 ttmvvvseke teytqvsdvk gayasteftt qvapmleevr shspteeylp dqkptsgiaq
    13141 pshvplesit vavqelaeke dlyktdvrpd qktanvalte ltsisipevm qvitassaet
    13201 lkketkmkee kgrveveels stiisevisn etedvfttka tpkdqkadfn isgreaaett
    13261 qvtamteaed lavkrpeeqk gkpqvdeltp ltvshvetge aekelptaev pkektaqpni
    13321 fgrdvaetmq vltvtsaeel aapkapegqk gkpqveeltp lsvsqvvsqe aesalpapea
    13381 pterkaqpdm tgrdiaetme vltmtsadil aedvkpteqk gmpgleefvp lsvsqtvste
    13441 kedvlpspea pttktaqpsl tgrdiaetmq vvmmtsekel italapethk gipnveeltp
    13501 ltvsqtvshe aedvfektaa psevtakpaf sgrevaqtse mmivtnvgel ekqvlpdeqk
    13561 gqaqldelmp ltvsqvisge veeglpeaek pkektaqpgi lgrdiaettq vltvtsaeel
    13621 aapkapegqk gkpqveelap ltvsqvvsee aegilpapea pterkaqpdm tgrdiaqtme
    13681 vltmtsvdtf tedkkpdeqk ghpeleefvp lsvtqtvsne medvlpspea pstkiaqpsl
    13741 tgrdvaetmq iltmtseeel itaqtpeqhk gkpnveeltp ltvsqtvsqe aeeilekaaa
    13801 ptevtakpaf sgrevaqtse imivtnvgel ekqvlpdeqk gqpqldeltp ltisqiisge
    13861 veeglpeaek pkektaqpgi lgrdiaettq vltvtsaeel eapkapegqk gkpqveelap
    13921 ltvsqvvsqe aegilpvpea pterkaqadl tgrdvaemme vltmasvdtl tedkkpaeqk
    13981 gvpgleefvp lsvsqtvste keevlpspea pttkvaeasl tgrdiaetmq vvtmtseeel
    14041 isarvpeqhk gifnveelts ltvsqtishe teeilekaaa lgevtakpaf tgrevaqtse
    14101 tlivtnvgel ekqilpeeqk gkprvdemts itvsqvvsge veerlpeaek pkektaqpsm
    14161 lgrdiaettq vltmtsaeel aapkapeeqk gkpqveelap ltvsqvvsqe aegvlpvpea
    14221 pterqaqpdl tgrdvaqitv vmsmasadvm iddkkpeeqk gvpgveefvp lsvsqtvste
    14281 kedvlpspea pttkmaqpsl tgrdiaetmq vvtmtseeel italapethk gkpnveelts
    14341 ltvsqtisqe tevileksaa pseatakpaf tgreiaqtse ilavlnvgel ekhvlpeeqk
    14401 gkpkleeltp ltvsqiisge aeeglptaev pkektaepsm lgrdiaettq vltmasaeel
    14461 mapkapeeqk gkpqveelap ltisqvvsqe aegvlpvpea pterkaqpdl tgrdvaqttv
    14521 vmsmanvdim ieekkpeeqr gipgleefvp lsvsqtvste kedvlpspev pttkmaqpsl
    14581 tghdvaetlq vltmvseeql ttkapeeqkg kpnveeltsl tvsqtvshea edilekavap
    14641 seatakpafl grevaetsqv ltvttvgelq kdvlpeeqkg kpqleelmpl tvsqivsgea
    14701 eerlpeaekp kektaepsml grdiaettqv ltmasaeell apkvpeeqkg kpqveelapl
    14761 tvsqvvsqea egtlpipeap tertaqpdlt grdvaetmev ltmasldtli edkkpaaqtg
    14821 apgleefipl svsqmvstek edvlpspevp tekmaqpsli grdvaetmqv ltmisamelt
    14881 tghapdqqkg vpnveeltsl tvsqtvshet eeilekavap stvtakaals grevaetsqv
    14941 ltvsnveefe ksilpegqkg kprldeltsi tvsqvlsnel eetlpspekp sekiaqpqmi
    15001 grevaeqtev ltvtnveelk klekpegqtg kpdveemsfi tvsevfstem ekempspevp
    15061 kertalpqls sievaettev ltmisaeell kskapeeqkv qpkldeltpl tvshvvseea
    15121 eealptaeip kektaqpsll grdvaetsqv ltmasaeell aakvpeeqkg kpqieelapl
    15181 tvsqvvsqea eealpsaevp vereaqpsmt srdvaqtmlv tsmsnvdima eskipeeqkg
    15241 vpgleefvsl svsqtvstek edvlpspevp tekmaqpsli grdvaettqi ltmisatelt
    15301 tdrapeqqkg vpnveeltsl tvsqavshet eeilekleap gtvtakaals grevaetsqv
    15361 ltvsnveefe ksvlpeeqkg kprldeltsi tvsqvvsnel eetlpspekp sekvaqpqmi
    15421 grevaektev ltvsnveemq klekpegqkg ipdveemsfi tvsevvstea ekelpapeap
    15481 kerkalpqls gievaeksev ltmtmteefa kpkapeeqkg kpqleellsl svsevalgev
    15541 ekglptpdkp teetakpsll giqvaeksqv iassitgelq esikpdtkqi ipeqipfesi
    15601 eqsqtltqer estfvvektt tsataevsfr vseslevtqv tatekeskev ikgvakeasa
    15661 qadvierkva lktevqpedm asefkvkkpe gktakgvdei qqgvivtehl tahefeselp
    15721 esvlpfakla rvsiqadhle hivetteapa qqhhititqh ttkhdlpqqq siesdtevie
    15781 eyttkfrrgs tedataavkt kktivrkkpk ttgeedivvv ieeelddikp kipsipkkqf
    15841 mpieevttvs dveeivsari deaeeivpke eekvevtite vqdtdkkkkv tkkqfvrrrg
    15901 ktrqvteeit veeegkapet tvvtkpveii eedieerpki ekeeyvveev peektiplkp
    15961 rkkltptvqq veeqvtvtee itkegkprkt tkkkvitrkg keqqvtevvt veeqgkapit
    16021 svtegpveev veeiiqklpa pkyakpseve evreqvtvte evtrlgkpkk ttkkkiikrk
    16081 gkeqqvtevv tveeegkapt ttvtegpvee vvieeikelp ipefvkptev eevreqvtvt
    16141 eeltkmgkpk ktikkkiikr kgkeqqvtev vtveeqgkap vttvkegpve evveetitpl
    16201 lapeyakpse veevreqvtv tqevtklgkp kkttkkkiik rkgkeqqvte ivtveeegka
    16261 pkttvtegpv eeiieeeikp efikprevee vreqvtvtqe vtregkpkkt ikkkiikkkg
    16321 keqqvtevvt veeqgkapvt tvtegpveev vdetvkalpa peyakpseve evreqvtvtq
    16381 evtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk ttvtegpvee iieeeikpef
    16441 ikpreveevr eqvtvtqevt regkpkktik kkiikkkgke eqvtevvtve eqgkapvttv
    16501 negpveevvd etvkalpape yaepseveev reqvtvtqev tklgkpkktt kkkiikrkgk
    16561 eqqvteivtv eeegkapktt vtegpveeii eeeikpefik preveevreq vtvtqevtre
    16621 gkpkktikkk iikkkgkeeq vtevvtveeq gkapvttvne gpveevvdet vkalpapeya
    16681 epseveevre qvtvtqevtk lgkpkkttkk kiikrkgkeq qvteivtvee egkapkttvt
    16741 egpveeiiee eikpefikpr eveevreqvt vtqevtregk pkktikkkii kkkgkeqqvt
    16801 elvtveeqgk apvttvtegp veevvdetvk alpapeyakp seveevreqv tvtqevtklg
    16861 kpkkttkkki ikrkgkeqqv teivtveeeg kapkttvteg pveeiieeei kpefikprev
    16921 eevreqvtvt qevtregkpk ktikkkiikk kgkeqqvtev vtveeqgkap vttvtegpve
    16981 evvdetvkal papeyakpse veevreqvtv tqevtklgkp kkttkkkiik rkgkeqqvte
    17041 ivtveeegka pkttvmegpv eevvieeiqe lpipefvkpt eveevreqvt vteevtrlgk
    17101 pkkttkkkii krkgkeqqvt eivtveeqgk apvttvtegp veevvdetik slpapeyakp
    17161 seveevreqv tvtqevtklg kpkkttkkki vkrkgkeqqv teivtveeeg kapkttvteg
    17221 pveevveeev kpllapefvk pseveevheq vtvtqevtre gkpmktikkk iikkkgkaqq
    17281 vtevvtveee gkvpvttvke gpveevvdet vkalpapkya kpseveevre qvtvtqeitk
    17341 lgkpkkttkr kvikrkgkeq qvtevvtvee egkapettvt egpveevlee eikplpgpef
    17401 vkpseveevr eqvtvteevt regkpkktik kkiikkkgke qqvteivtve eqgkapvtsv
    17461 tegpveevvd etikqlpapk yakpseveev reqvtvtqem tklgkpkktt kkkiikrkgk
    17521 eqqvteivtv eeegkapktt mtegpveevv eeeikplpal efvkpsevee vreqvtvtee
    17581 vtregkpkkt ikkkiikkkg keqqitevvt veeqgkapvt tvtkapveei vdetikelpa
    17641 lefakpseve evreqvtvtk evtkegkpkk tikrkvikkk gkeqqvteiv tveeegkapk
    17701 ttitegpvee vveeeikplp alefvkpsev eevreqvtvt eevtregkpk ktikkkiikk
    17761 kgkeqqvtev vtveeqgkap vttvqegpve evidetiksl papeyakpse veeireqvtv
    17821 teeltklgkp kkttkkkitk rkgpeqqite ivtveeegka pvttvtegpv eevvkevlkp
    17881 lpvpkvvkps eveeireqvt vteemtkegk pkkiikkkvi krkgreqtvq evvtveekgk
    17941 apvttvtvgp teeiveetvk tlpapeyakp seveevreqv tvtqevtkeg kpkkttkkkv
    18001 ikrkgkeqqv tevvtveeqg kapvttvteg pveevveell kpliapeyvk pseveevreq
    18061 vtvteeltke gkpkkvikkk vikrrgreqq vkevvtveeq gkapvttvte gpteeiveet
    18121 ikalpapeda kpseveevre qvtvtqevtk egkpkktikk kvtkrkgkeq qvtevvtvee
    18181 kgkapvttvt egpveeivee tvkalpapel vkptevtevp eeltvtevvt kegktkkivk
    18241 kkvpkpkkeg ieapeeapee vpveetkkii klqrlkvskf estkldiqet slgkpqfaqi
    18301 klrktpvakr pskkdekfpr illrsriiai ewppspknlk vdtfepielq ngilsrnvde
    18361 aaalpkpkkk kikriekeia klekldqefe elkkelplek leepileekp erkvkkkkkp
    18421 edkakegvkp qlmrisikeq kpikpkiaep atplfaqikl kkapakpkkk peeevskfpk
    18481 illrsritah ewppsvkypa isvlepnyiq ngilsrtmee alklkkvkhk kmklpekelt
    18541 elekldqefe elkkvplekv eeitpyerkp kpekpeeekp kklvigkgkr rpaeeedlet
    18601 vqlkkipeki psapeeaekv pkkkdevdkp kkekeekeyp elkpfepydi eapeveleer
    18661 ekpeypepek pkekkvpgkk pkkerktpaq eteavpivpg vpkpkepeee eeikrriper
    18721 pppteepeki klkpwkkgek peeeekkped yikfvpkddd ivetvevttt veetpdkkqr
    18781 kikkkktitk pkdekpkiie ettieeegfe pvvtveefdv dkyeavpves vekaapveei
    18841 keeivtseii teegtkktvr kkrvtkkreg kervteevtv eeegkepita tielhedtis
    18901 eiheqakhkr ptkpkadekl aedvtektve qqeiieevke vkptkkvikk ekpvkakdev
    18961 teevveeiii eepekqeakp iveeeetvii tehdvrkapk kkkevvikee eeifetietp
    19021 thkiirkkir pmkkgekevv veeiiqkpre etvemeeqqi vevvetptkk ivkkkkevvk
    19081 ddgvpeelve eiiiekpqkk kakptveeek kkvkvtkvdt kkapekpeev veeeeeiiet
    19141 ietptkkiir kkpkdkkdki ieeviqktie eakameeqei vevietptkk vikkktttie
    19201 egkapeekve eiviekpkev ieeeeeiiet ietptkrivr kkpkdkkdki veeviqktie
    19261 eaktmkeqei ievietptkt vikkkkatik egkapeeeve eiviekpike kakkkilpke
    19321 gveitevite tsveelieee lkpeeakpse eveeekvkit evaikkvpkk kkpskaveae
    19381 ekeevveeik pekpkeekak ptvtekegie vtevisevap eklpeeakpk kkkateiket
    19441 eekvitkkvk vkeapkeavp geeeqpaeev delvpekpkk tkakkevtpq dsivttevvp
    19501 itkeeeleve kpeeekatvv eekeekakve ekkvtvkkap kkklkpteae eeqveevvee
    19561 itpeepkkrk akkavipkee iqttevipgi taeeipeeek peeekavpke eekveeeakv
    19621 tevsvkkker kkkekpvtng evieekpkvk eipeekpkee vpqvpeekek peeekpkevk
    19681 pkekkpkeek pkkpieekpe evkpeeekpe vkpeeekpke ekpkkkkpae kkpkiseeer
    19741 pedkveeipe kkpeevktre eiipkdtvqt tevipetvae eiptkkpker kavpveekee
    19801 kveitevpve kapekkeekv peekpteaap ekpkeakvmk kkkkkpakkd eieewvepey
    19861 erpvlepmpe kivwepkkkk ekkplpeslq klvpqkierk emkptklkys epaetvqfas
    19921 iklkkvavpk kkeadetkfp kimlrsrmtf vgdyppelqi pkityleenp vqtgilsrnt
    19981 eealkvlkrk vkkvklseke vtqlekldle feelkkvple kiedksvyer qpkkpaekpe
    20041 epkkvpigkg kvpeeekvep ekvklkkvpe kkpeeveepl kkpkkpepee evkpekkkkp
    20101 kvklehdelk pldfdrpele kyepeeyekp eepapepvpk pyekepkkkp dqeveqiplk
    20161 kgkpkspepd eepevkfrip tkekpeeepe qitlkpwkke kpeeekekei pkkeeekvpk
    20221 ppkeepedvt vqrkaktkkp kekpkeekee evtvkkpeee kpeevaeevt lkkepekpve
    20281 eapaevtikk pvveekevee eetitevilk kkpekpeevv eeavvkkpkk kkpvkeeevv
    20341 eevtvtkvvt eeekeeekvv ikkpkkksip eiteevteei tlkkpeekap eevteeikkp
    20401 kkkkpvkeev aeeitikkpv veekkeeevp eevtlkpkkp eekpvieevt eeitlkkpvp
    20461 eevteevaik kpkkkkpvee eaadeitlkk pvvvekeeee iteevtlqkk kkpekkpvpe
    20521 eiseeitlkk pvpkeeekap eevteeiaik kpkkkkpvke eaadevtikk pvveekkeee
    20581 tpeevtlkpr kpkekpvpee vkeevtlkkp vpeekekvpe evteeiaikk pkkkkpveee
    20641 aadevtikkp vvvekeeeei teeitlgkkk kpekkpvpee iteeitlkkp vpkeeekape
    20701 evtekievkk pkkkkpveee aadevtikkp vvvekeeeea paeitlkrrp kekpveeite
    20761 evteeitikk ptpeekekap eevsekvevk kpkkkkpvve eaadevtikk vvveekkeee
    20821 apeevtlkpr pkkksvteee vaevtltkpk pieeekkpee seltikkkpk kkpvieeeaa
    20881 evtikkpvpv epdeapeeit irkkkkpekk pkvteeveet avtikkarap ekpteeeeit
    20941 tdvqikkkkp qkkveevaad eltikkieev eqpeeeeevq eftfkrkppk lppkpieeiy
    21001 edvtlrklrp krksrpdike itetenvtfr prttktkedv eqefkislnt yeeedismsg
    21061 kvrlkpkkkp ltyseetgee tiriiqeved dsgpiieeii esdeegkdey sieeldtdem
    21121 rlplrrkkkk vpkpykvedi eegdvklklk rerkysieea deslalklks krrvstydee
    21181 eatlsitree diseeveiey vvrdgdtmfs icsyvaetde ainlvegerv yvidhtnqdw
    21241 wfvkkhltee kgwvpaqyll nevhytlylq rklhekidkl pvfekpapgd kssapkfvek
    21301 lqpihtpdgy tiqfecqveg vprpqitwfr qtaiikpspd fqmyydddnv atliirevfp
    21361 edagtftcva knaagfasst teliveapls dhgsdltgps rkslsressl adilegippt
    21421 fsrkpkakyv degedvilec rlvavpepei twyykdtqit tkeniiiate sdmhmycsvi
    21481 kitkvqkkqe gkytivarnr egeatieipm kvktgksepp eileplqsyv vregetvvls
    21541 tqivgnpapk vtwfkngkpl kgltpkqdgh vntltliqpq vsdtgeysvv atndlgtaet
    21601 katltveinl kpslainfqq htfehfsvtk eipsgapepp lfterfqelt vpekgtfklv
    21661 akvtgnpvpe vtwlrnnkpl ekspnitety dgenivleir nansevdtgd ykcvasnpvg
    21721 kashgarvtv dvdkvtftkt lekevvvdey ktleltcets htvstkwwhn dkeisgmdhr
    21781 eiieegrvhk lvikktnptd egtykctvkn qstssnvtvk atkpefvkkl qdcevkerdv
    21841 aileveitsq tvdvkwykdg eqlgpgkekl efvkegtvrk llirstsvhd egeytcslld
    21901 qectaevtvi elppeiitkm qdvtiarger atfdieltkg dalvrwfkdg qelqfsehvr
    21961 lsidgkrqkl kiydteseda gvyscevgqq kssakltvee pavefitklp dvtlvplnsd
    22021 avfvielsqd vpvtwmrktq vikespkyti idegkvkkli vkkcttedis eytamvanvk
    22081 tssrlrvevi etppkispdt prkykvrkde dvdivvkfsg tpkpedewtv ngrvvtkskr
    22141 aiptideesa vltirkiqee dvgdytlklv nsvgetsiei nvvivqvpsa pgapeplett
    22201 ndsvtlhwkk pdsdgnspiv eyilehqekt ettwtkitet itetthkvtk lttnkeytfr
    22261 vtavneagpg etsptspyvk iskpstaepp vileplksiv iglgetvtls cviggtpape
    22321 itwlrndeif edtsityenh itkytirett etssakftvk aknnvgsaet tcelkiqesp
    22381 kiiydenltn qklpmngqwk ieiqitgfpk pevtwtkngk evdekrvsih reertstvsi
    22441 sslvredtat ymakaenqag sssvdlhlkv idkpskpqgp vvfkeirqdr vtlewrppad
    22501 dggielekyt iekcepgktw vkvvdidkev esfcvhklqq naeykfriia rnavgasepl
    22561 esdtvkmrts feppgaprgp levsgmtkts ftikwqppen dggtpitdyi ieikeeskkt
    22621 wqkigstkhe tthfsvsdlk tdvpynfrit aknsvgsgpp yvaeepiapg rrikitklte
    22681 ssmyallgif pptkiviskt ppsspqhvhv tnitsksatl swsppastgg teltgyviek
    22741 rpligkgarw tkvvtldatt lqycvenlke seflfrifae nnqglsvpan sepvtlktha
    22801 svpspptapl eirqiaantv viswgrpdsd ggaplegykv airdtkrtmw mevarvntei
    22861 qklnirdlqe nhmylirifa rneiglsdpl eseepfkiip aseltvvepi aeatekgeta
    22921 sisfstents swlrehnmda dihsyararl lrqdeyffri whyakklfe
//
LOCUS       XP_034182876           21728 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X13 [Osmia lignaria lignaria].
ACCESSION   XP_034182876
VERSION     XP_034182876.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326985.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182876.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..21728
                     /product="sallimus isoform X13"
                     /calculated_mol_wt=2442961
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          10008..10099
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          10025..10029
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          10038..10042
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          10065..10069
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          10079..10084
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          10092..10095
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          10114..10203
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          10131..10135
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          10144..10148
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          10169..10173
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          10183..10188
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          10198..10201
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          10216..10302
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          10233..10237
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          10246..10250
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          10271..10275
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          10285..10290
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          10298..10301
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          10319..10409
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          10336..10340
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          10349..10353
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          10375..10379
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          10389..10394
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          10402..10405
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          10457..10543
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          10476..10480
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          10489..10493
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          10515..10519
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          10529..10534
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          10581..10672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          10598..10602
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          10611..10615
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          10638..10642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          10652..10657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          10665..10668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <14160..>14312
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          19966..20018
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(19971,19973,19979..19980,19997..19999,20012,20014,
                     20016..20017)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          20054..20144
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20071..20075
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20084..20088
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20110..20114
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20124..20129
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20137..20140
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20178..20271
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20195..20199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20208..20212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20233..20241
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20251..20256
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20264..20267
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20279..20365
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20296..20300
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20309..20313
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20331..20335
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20345..20350
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20358..20361
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20399..20489
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20416..20420
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20429..20433
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20454..20458
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20469..20474
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20482..20485
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20502..20579
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          20512..20516
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20523..20527
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20548..20552
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20562..20567
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20583..20668
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20600..20604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20612..20616
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20638..20642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20652..20657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20661..20664
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20673..20757
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20690..20694
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20702..20706
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20727..20731
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20741..20746
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20750..20753
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20863..20943
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20871..20875
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20884..20888
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20909..20913
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20923..20928
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20936..20939
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20947..21038
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20947,21012,21027)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(21028..21029,21031..21032)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          21049..21135
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          21066..21070
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21079..21083
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21101..21105
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21115..21120
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21128..21131
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21159..21229
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          21159..21162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          21171..21175
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          21195..21199
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          21209..21214
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          21222..21225
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          21233..21325
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(21233,21299,21314)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <21303..21618
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(21315..21316,21318..21319)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..21728
                     /gene="sls"
                     /coded_by="XM_034326985.2:753..65939"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
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     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk keeegiaphf stpikplmve
    10021 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    10081 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    10141 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    10201 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    10261 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    10321 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    10381 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    10441 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    10501 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    10561 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    10621 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    10681 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    10741 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    10801 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    10861 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    10921 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    10981 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    11041 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    11101 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    11161 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    11221 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    11281 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    11341 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    11401 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    11461 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    11521 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    11581 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    11641 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    11701 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    11761 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    11821 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    11881 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    11941 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    12001 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    12061 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    12121 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    12181 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    12241 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    12301 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    12361 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    12421 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    12481 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    12541 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    12601 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    12661 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    12721 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    12781 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    12841 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    12901 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    12961 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    13021 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    13081 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    13141 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    13201 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    13261 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    13321 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    13381 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    13441 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    13501 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    13561 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    13621 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    13681 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    13741 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    13801 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    13861 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    13921 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    13981 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    14041 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    14101 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    14161 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    14221 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    14281 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    14341 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    14401 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    14461 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivetteapaq qhhititqht
    14521 tkhdlpqqqs iesdteviee yttkfrrgst edataavktk ktivrkkpkt tgeedivvvi
    14581 eeelddikpk ipsipkkqfm pieevttvsd veeivsarid eaeeivpkee ekvevtitev
    14641 qdtdkkkkvt kkqfvrrrgk trqvteeitv eeegkapett vvtkpveiie edieerpkie
    14701 keeyvveevp eektiplkpr kkltptvqqv eeqvtvteei tkegkprktt kkkvitrkgk
    14761 eqqvtevvtv eeqgkapits vtegpveevv eeiiqklpap kyakpsevee vreqvtvtee
    14821 vtrlgkpkkt tkkkiikrkg keqqvtevvt veeegkaptt tvtegpveev vieeikelpi
    14881 pefvkpteve evreqvtvte eltkmgkpkk tikkkiikrk gkeqqvtevv tveeqgkapv
    14941 ttvkegpvee vveetitpll apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    15001 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    15061 tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt vtegpveevv detvkalpap
    15121 eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    15181 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    15241 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    15301 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    15361 reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv tevvtveeqg kapvttvneg
    15421 pveevvdetv kalpapeyae pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    15481 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    15541 kktikkkiik kkgkeqqvte lvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    15601 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp
    15661 veeiieeeik pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv
    15721 tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk
    15781 kttkkkiikr kgkeqqvtei vtveeegkap kttvmegpve evvieeiqel pipefvkpte
    15841 veevreqvtv teevtrlgkp kkttkkkiik rkgkeqqvte ivtveeqgka pvttvtegpv
    15901 eevvdetiks lpapeyakps eveevreqvt vtqevtklgk pkkttkkkiv krkgkeqqvt
    15961 eivtveeegk apkttvtegp veevveeevk pllapefvkp seveevheqv tvtqevtreg
    16021 kpmktikkki ikkkgkaqqv tevvtveeeg kvpvttvkeg pveevvdetv kalpapkyak
    16081 pseveevreq vtvtqeitkl gkpkkttkrk vikrkgkeqq vtevvtveee gkapettvte
    16141 gpveevleee ikplpgpefv kpseveevre qvtvteevtr egkpkktikk kiikkkgkeq
    16201 qvteivtvee qgkapvtsvt egpveevvde tikqlpapky akpseveevr eqvtvtqemt
    16261 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttm tegpveevve eeikplpale
    16321 fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk eqqitevvtv eeqgkapvtt
    16381 vtkapveeiv detikelpal efakpsevee vreqvtvtke vtkegkpkkt ikrkvikkkg
    16441 keqqvteivt veeegkapkt titegpveev veeeikplpa lefvkpseve evreqvtvte
    16501 evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv ttvqegpvee videtikslp
    16561 apeyakpsev eeireqvtvt eeltklgkpk kttkkkitkr kgpeqqitei vtveeegkap
    16621 vttvtegpve evvkevlkpl pvpkvvkpse veeireqvtv teemtkegkp kkiikkkvik
    16681 rkgreqtvqe vvtveekgka pvttvtvgpt eeiveetvkt lpapeyakps eveevreqvt
    16741 vtqevtkegk pkkttkkkvi krkgkeqqvt evvtveeqgk apvttvtegp veevveellk
    16801 pliapeyvkp seveevreqv tvteeltkeg kpkkvikkkv ikrrgreqqv kevvtveeqg
    16861 kapvttvteg pteeiveeti kalpapedak pseveevreq vtvtqevtke gkpkktikkk
    16921 vtkrkgkeqq vtevvtveek gkapvttvte gpveeiveet vkalpapelv kptevtevpe
    16981 eltvtevvtk egktkkivkk kvpkpkkegi eapeeapeev pveetkkiik lqrlkvskfe
    17041 stkldiqets lgkpqfaqik lrktpvakrp skkdekfpri llrsriiaie wppspknlkv
    17101 dtfepielqn gilsrnvdea aalpkpkkkk ikriekeiak lekldqefee lkkelplekl
    17161 eepileekpe rkvkkkkkpe dkakegvkpq lmrisikeqk pikpkiaepa tplfaqiklk
    17221 kapakpkkkp eeevskfpki llrsritahe wppsvkypai svlepnyiqn gilsrtmeea
    17281 lklkkvkhkk mklpekelte lekldqefee lkkvplekve eitpyerkpk pekpeeekpk
    17341 klvigkgkrr paeeedletv qlkkipekip sapeeaekvp kkkdevdkpk kekeekeype
    17401 lkpfepydie apeveleere kpeypepekp kekkvpgkkp kkerktpaqe teavpivpgv
    17461 pkpkepeeee eikrriperp ppteepekik lkpwkkgekp eeeekkpedy ikfvpkdddi
    17521 vetvevtttv eetpdkkqrk ikkkktitkp kdekpkiiee ttieeegfep vvtveefdvd
    17581 kyeavpvesv ekaapveeik eeivtseiit eegtkktvrk krvtkkregk ervteevtve
    17641 eegkepitat ielhedtise iheqakhkrp tkpkadekla edvtektveq qeiieevkev
    17701 kptkkvikke kpvkakdevt eevveeiiie epekqeakpi veeeetviit ehdvrkapkk
    17761 kkevvikeee eifetietpt hkiirkkirp mkkgekevvv eeiiqkpree tvemeeqqiv
    17821 evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk akptveeekk kvkvtkvdtk
    17881 kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii eeviqktiee akameeqeiv
    17941 evietptkkv ikkktttiee gkapeekvee iviekpkevi eeeeeiieti etptkrivrk
    18001 kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv ikkkkatike gkapeeevee
    18061 iviekpikek akkkilpkeg veitevitet sveelieeel kpeeakpsee veeekvkite
    18121 vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp tvtekegiev tevisevape
    18181 klpeeakpkk kkateikete ekvitkkvkv keapkeavpg eeeqpaeevd elvpekpkkt
    18241 kakkevtpqd sivttevvpi tkeeelevek peeekatvve ekeekakvee kkvtvkkapk
    18301 kklkpteaee eqveevveei tpeepkkrka kkavipkeei qttevipgit aeeipeeekp
    18361 eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge vieekpkvke ipeekpkeev
    18421 pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee vkpeeekpev kpeeekpkee
    18481 kpkkkkpaek kpkiseeerp edkveeipek kpeevktree iipkdtvqtt evipetvaee
    18541 iptkkpkerk avpveekeek veitevpvek apekkeekvp eekpteaape kpkeakvmkk
    18601 kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke kkplpeslqk lvpqkierke
    18661 mkptklkyse paetvqfasi klkkvavpkk keadetkfpk imlrsrmtfv gdyppelqip
    18721 kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev tqlekldlef eelkkvplek
    18781 iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe kvklkkvpek kpeeveeplk
    18841 kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek yepeeyekpe epapepvpkp
    18901 yekepkkkpd qeveqiplkk gkpkspepde epevkfript kekpeeepeq itlkpwkkek
    18961 peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk ekpkeekeee vtvkkpeeek
    19021 peevaeevtl kkepekpvee apaevtikkp vveekeveee etitevilkk kpekpeevve
    19081 eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi kkpkkksipe iteevteeit
    19141 lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv eekkeeevpe evtlkpkkpe
    19201 ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee aadeitlkkp vvvekeeeei
    19261 teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape evteeiaikk pkkkkpvkee
    19321 aadevtikkp vveekkeeet peevtlkprk pkekpvpeev keevtlkkpv peekekvpee
    19381 vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit eeitlgkkkk pekkpvpeei
    19441 teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea adevtikkpv vvekeeeeap
    19501 aeitlkrrpk ekpveeitee vteeitikkp tpeekekape evsekvevkk pkkkkpvvee
    19561 aadevtikkv vveekkeeea peevtlkprp kkksvteeev aevtltkpkp ieeekkpees
    19621 eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti rkkkkpekkp kvteeveeta
    19681 vtikkarape kpteeeeitt dvqikkkkpq kkveevaade ltikkieeve qpeeeeevqe
    19741 ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei tetenvtfrp rttktkedve
    19801 qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet iriiqevedd sgpiieeiie
    19861 sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie egdvklklkr erkysieead
    19921 eslalklksk rrvstydeee atlsitreed iseeveieyv vrdgdtmfsi csyvaetdea
    19981 inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln evhytlylqr klhekidklp
    20041 vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv prpqitwfrq taiikpspdf
    20101 qmyydddnva tliirevfpe dagtftcvak naagfasstt eliveaplsd hgsdltgpsr
    20161 kslsressla dilegipptf srkpkakyvd egedvilecr lvavpepeit wyykdtqitt
    20221 keniiiates dmhmycsvik itkvqkkqeg kytivarnre geatieipmk vktgkseppe
    20281 ileplqsyvv regetvvlst qivgnpapkv twfkngkplk gltpkqdghv ntltliqpqv
    20341 sdtgeysvva tndlgtaetk atltveinlk pslainfqqh tfehfsvtke ipsgapeppl
    20401 fterfqeltv pekgtfklva kvtgnpvpev twlrnnkple kspnitetyd genivleirn
    20461 ansevdtgdy kcvasnpvgk ashgarvtvd vdkvtftktl ekevvvdeyk tleltcetsh
    20521 tvstkwwhnd keisgmdhre iieegrvhkl vikktnptde gtykctvknq stssnvtvka
    20581 tkpefvkklq dcevkerdva ileveitsqt vdvkwykdge qlgpgkekle fvkegtvrkl
    20641 lirstsvhde geytcslldq ectaevtvie lppeiitkmq dvtiargera tfdieltkgd
    20701 alvrwfkdgq elqfsehvrl sidgkrqklk iydtesedag vyscevgqqk ssakltveep
    20761 avefitklpd vtlvplnsda vfvielsqdv pvtwmrktqv ikespkytii degkvkkliv
    20821 kkcttedise ytamvanvkt ssrlrvevie tppkispdtp rkykvrkded vdivvkfsgt
    20881 pkpedewtvn grvvtkskra iptideesav ltirkiqeed vgdytlklvn svgetsiein
    20941 vvivqvpsap gapeplettn dsvtlhwkkp dsdgnspive yilehqekte ttwtkiteti
    21001 tetthkvtkl ttnkeytfrv tavneagpge tsptspyvki skpstaeppv ileplksivi
    21061 glgetvtlsc viggtpapei twlrndeife dtsityenhi tkytirette tssakftvka
    21121 knnvgsaett celkiqespk iiydenltnq klpmngqwki eiqitgfpkp evtwtkngke
    21181 vdekrvsihr eertstvsis slvredtaty makaenqags ssvdlhlkvi dkpskpqgpv
    21241 vfkeirqdrv tlewrppadd ggielekyti ekcepgktwv kvvdidkeve sfcvhklqqn
    21301 aeykfriiar navgaseple sdtvkmrtsf eppgaprgpl evsgmtktsf tikwqppend
    21361 ggtpitdyii eikeeskktw qkigstkhet thfsvsdlkt dvpynfrita knsvgsgppy
    21421 vaeepiapgr rikitkltes smyallgifp ptkivisktp psspqhvhvt nitsksatls
    21481 wsppastggt eltgyviekr pligkgarwt kvvtldattl qycvenlkes eflfrifaen
    21541 nqglsvpans epvtlkthas vpspptaple irqiaantvv iswgrpdsdg gaplegykva
    21601 irdtkrtmwm evarvnteiq klnirdlqen hmylirifar neiglsdple seepfkiipa
    21661 seltvvepia eatekgetas isfstentss wlrehnmdad ihsyararll rqdeyffriw
    21721 hyakklfe
//
LOCUS       XP_034182884           21030 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X14 [Osmia lignaria lignaria].
ACCESSION   XP_034182884
VERSION     XP_034182884.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182884.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21030
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..21030
                     /product="sallimus isoform X14"
                     /calculated_mol_wt=2376500
     Region          140..229
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          157..161
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          170..174
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          192..199
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          209..214
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          222..225
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          236..326
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          253..257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          266..270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          292..296
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          306..311
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <961..1232
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1947..2036
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1964..1968
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1977..1981
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2002..2006
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2016..2021
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2029..2032
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2193..2280
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2210..2214
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2223..2227
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2250..2254
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2264..2269
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2390..2479
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2407..2411
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2420..2424
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2445..2449
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2459..2464
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2472..2475
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2515..2605
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2532..2536
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2545..2549
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2571..2575
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2585..2590
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2598..2601
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2614..2704
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2631..2635
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2644..2648
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2670..2674
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2684..2689
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2697..2700
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2761..2852
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2778..2782
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2793..2797
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2819..2822
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2832..2837
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2845..2848
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2894..2986
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2912..2916
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2927..2931
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2966..2971
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2979..2982
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3125
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3051..3055
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3066..3070
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3091..3095
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3105..3110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3118..3121
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3167..3259
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3185..3189
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3200..3204
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3226..3230
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3239..3244
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3252..3255
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3301..3393
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3319..3323
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3334..3338
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3359..3363
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3373..3378
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3386..3389
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3435..3526
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3452..3456
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3467..3471
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3493..3496
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3506..3511
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3519..3522
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3568..3658
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3586..3590
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3601..3605
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3626..3630
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3640..3645
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3653..3656
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3702..3793
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3719..3723
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3730..3738
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3759..3763
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3773..3778
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3786..3789
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3835..3926
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3852..3856
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3867..3871
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3892..3896
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3906..3911
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3919..3922
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3968..4060
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3986..3990
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4001..4005
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4026..4030
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4040..4045
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4053..4056
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4102..4194
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4120..4124
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4133..4139
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4160..4164
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4174..4179
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4187..4190
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4234..4326
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4252..4256
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4265..4271
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4292..4296
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4306..4311
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4319..4322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4367..4459
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4385..4389
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4398..4404
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4425..4429
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4439..4444
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4452..4455
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4501..4593
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4519..4523
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4534..4538
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4559..4563
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4573..4578
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4586..4589
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4633..4725
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4666..4670
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4691..4695
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4705..4710
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4767..4859
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4785..4789
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4800..4804
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4825..4829
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4839..4844
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4852..4855
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4899..4989
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4916..4920
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4931..4935
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4956..4960
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4970..4975
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4983..4986
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <5066..5126
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5070..5074
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5095..5099
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5109..5114
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5123..5126
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5174..5265
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5207..5211
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5232..5236
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5246..5251
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5275..5366
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5293..5297
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5308..5312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5330..5337
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5347..5352
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5360..5363
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5408..5499
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5426..5430
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5441..5445
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5463..5467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5480..5485
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5493..5496
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5546..5637
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5579..5583
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5604..5608
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5618..5623
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5684..5775
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5701..5705
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5716..5720
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5741..5745
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5755..5760
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5768..5771
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5821..5911
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5838..5842
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5853..5857
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5878..5882
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5892..5897
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5905..5908
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5954..6043
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5971..5975
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5986..5990
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6011..6015
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6025..6030
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6038..6041
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6097..6187
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6115..6119
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6130..6134
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6152..6156
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6169..6174
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6182..6185
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6235..6323
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6252..6256
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6265..6269
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6289..6293
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6303..6308
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6316..6319
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6558..6648
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6575..6579
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6588..6592
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6613..6617
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6627..6632
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6640..6643
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6662..6751
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6679..6683
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6692..6696
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6717..6721
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6731..6736
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6744..6747
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6772..6861
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6789..6793
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6802..6806
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6827..6831
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6841..6846
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6854..6857
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6960
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6887..6891
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6901..6905
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6926..6930
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6940..6945
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6953..6956
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7037..7734
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          7544..>7953
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          9211..9300
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9228..9232
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9241..9245
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9266..9270
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9280..9285
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9310..9401
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9327..9331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9340..9344
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9367..9371
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9381..9386
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9394..9397
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9416..9505
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9433..9437
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9446..9450
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9471..9475
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9485..9490
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9500..9503
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9518..9604
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9535..9539
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9548..9552
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9573..9577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9587..9592
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9600..9603
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9621..9711
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9638..9642
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9677..9681
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9691..9696
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9704..9707
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9759..9845
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9778..9782
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9791..9795
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9817..9821
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9831..9836
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9883..9974
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9900..9904
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9913..9917
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9940..9944
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9954..9959
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9967..9970
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <13462..>13614
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          19268..19320
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(19273,19275,19281..19282,19299..19301,19314,19316,
                     19318..19319)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          19356..19446
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19373..19377
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19386..19390
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19412..19416
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19426..19431
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19439..19442
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19480..19573
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19497..19501
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19510..19514
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19535..19543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19553..19558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19566..19569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19581..19667
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19598..19602
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19611..19615
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19633..19637
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19647..19652
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19660..19663
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19701..19791
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19718..19722
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19731..19735
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19756..19760
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19771..19776
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19784..19787
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19804..19881
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          19814..19818
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19825..19829
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19850..19854
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19864..19869
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19885..19970
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19902..19906
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19914..19918
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19940..19944
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19954..19959
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19963..19966
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19975..20059
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19992..19996
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20004..20008
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20029..20033
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20043..20048
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20052..20055
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20165..20245
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20173..20177
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20186..20190
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20211..20215
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20225..20230
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20238..20241
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20249..20340
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20249,20314,20329)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(20330..20331,20333..20334)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          20351..20437
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20368..20372
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20381..20385
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20403..20407
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20417..20422
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20430..20433
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20461..20531
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20461..20464
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20473..20477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20497..20501
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20511..20516
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20524..20527
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20535..20627
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20535,20601,20616)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <20605..20920
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(20617..20618,20620..20621)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..21030
                     /gene="sls"
                     /coded_by="XM_034326993.2:414..63506"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mhassssdkh fsaekseigg enkvltkmse titesstthk tlkkeyisss mssstgapgq
       61 eptsvcvkst vhsteqsssa dgappvvqsy kveehekiiq dqpgeirqer tvvvsqdeeg
      121 ikrdikatqi pkpvrkstap rfvspvtgmi vdqgsdivle giidgfpqpv viwskngqel
      181 ktkdsmtity shnhvrlelk nvnvkdagry tctasnevgs asstadlvvk ktifppvfgr
      241 rlqaqlvkrg drvnmeveit gtpeptvswf kddipikerp pelrikqqgn cymlimekaa
      301 kehagkymvr atnaggeaqs iadfavfept pdtmvevhkt viydvqdkkl mqsdekkaev
      361 psskhltseq vsttkiqpaa tikttptams tsmaqmsrtv ekikepdtel crsekisstv
      421 eshqmerkse qkyhmklehk mpsivepkte gktvikevir eevrssepks tlieekiene
      481 nietsriakk dalsffesmt retetipkgp kemiklvdda dghevkvgkl tknyekstsf
      541 qevkkpevkp pellttkkav heiftkleqg sipsrgidnk lfdfpyepyk ptmetkktvv
      601 eetissgsss lqssmtmskm eskreskmes kssellsegf nlvpepppei gympkpeevk
      661 kkrpevsika kqlqesfdkt lspidapigg vkifpspspk iletpkvapp sfsmppppfq
      721 lekkeiveev cikkdvhekr dpqplvrpis psrpwssssd vetrsqmstd lseyrchsaa
      781 sshqeilrss sprpsadgla mekswakkca dstrkswppq edtpikykqe wqmpgedykt
      841 sskefkreve etpeggikkt smessstter rswsskqetm dkmskrapsp pkiqpiiyna
      901 etikvdhtvn kieekslyek yisecdvkks qksekleeys skkwtgaedl kapglvrsle
      961 tpkkptpply rpvepiptdq iilepgpppe igyipapvvr ekkieqiekt levslehkpa
     1021 kippggirti ppapkkeepp alppkdvrit ppplpakqpt pveplepfpf kpspsptikt
     1081 pkvavpaptp skfvkgsfgv esdyesdmds hmkakwrpye sdneepryrr vqppvpkqpr
     1141 prstepeplp pskfevpses tglprpvltk eqqekmykkt ttfkrqeket kqqkyqvvqp
     1201 ppvelkpgsp piyvqpatks ptpksppakk pespkfkvkt fqqesgymad tdeplqqkss
     1261 vaslsksfgk qessssmssh mesrtsyses rseyfesksy dsrqqqqkke sfapsapssv
     1321 kqppqqrssf veksygssav tssprfmsak etlyttdqsh kkfeapkkil ppspspsrfv
     1381 ksefresdye sdydgrippl wkprgyesdd qsfrtvkpnl agpgkpkvqr edtsrlieea
     1441 straqkaktf ekreervatk pktvllpgsp peiayappqp qptyyearsg ipfhnaigte
     1501 tkktvrmdes tensrrivtv eqtsrvikfg dnqksatdfd sfdsssaqkq kthykvptpk
     1561 kfvqgqfres dyesdidtri kpkwappesd seepryrkvq ppttkttrss sapvrqehvv
     1621 spmefdtgpt vlrkqtsmsq lrderkfdgq vkrqassrqe etlkpgsppe fgyisrsdvk
     1681 kaanhvasrh msdmtssfks ktekfvtdiq sdlkqgkpil kqpvdgrasd adeprtyree
     1741 srlsehgtkq idpdtgliyf kydfgyefgi vlpgegkktv sstsrsvqgq rrasdievpi
     1801 vhefttrken gfakrsasss starqgkpag kfsgsktvkw eptseseyse sedirnarkr
     1861 hseggntmta pslvipcsps psrwdhttps plslspslps lspryssatg ppsnvdsags
     1921 pwpttngvas skeviqpyle ilpkkaplfi tplrdiavvs gqtarfeciv qaepqpnilw
     1981 skdgriiens tsyeihyrng vcrltivraf pedagtyact atnslgstvt satllvpgvp
     2041 spfygtyrdq nlsersaesp pkyvalrdlf lpqesdvkta ssnrqqqhns stfeyawsvn
     2101 rsyaggryre kenateecve itsrnpiarr leysdfqand sfrdvadfcf dreystcnps
     2161 iasprntvqg eivmqttptv paftgrpgdp sppvfeqifk narfaqggna ifegrvrgnp
     2221 kptvtwthkg aplleswkir msyddktgav tlqinqigpg degeytcsak nqygeaicsv
     2281 yiqpegfgpp pqqqiggyrk eyaqtfqste qksqtgsqsf qqrsyqqtid krsyvngtst
     2341 siedfkvdtf eyrllretef resitrrfvg esdvqistvv drslgpvapp qisqkprnsk
     2401 liegsdavft akisgnpkpr ltwfkngqri resqrvemsh snqqatlrir valpedsghy
     2461 tllsenpqgc tvssaylaie ssdqvdqayq tpresvktpq vestgesdsg kvlppnfvrt
     2521 ctdrdategk mtrfdcrvtg rpypevtwyi ngqqvandlt hkilvnesgn nslmitnvsr
     2581 adagvvtcva rnkagetsfq cnlnviekeq vvapkfverf tttnvkegep vvfmaravgt
     2641 pvpritwqkd gvpitpgpdv ristdgsgas tldipcakls daawyqctaq nvagstatra
     2701 rlfvetpkga apeprrlnlp rptkviepep apgpeviylr hverakpylp ppeedrvypp
     2761 prfiiplrdv hqieggrihf eariepvgdp tmrvewyvng raldassrat sifrfgfisl
     2821 dlisiilqds geyvcrvvss tgvaesratl svtpratieq asqhpdslqy iqqledysky
     2881 qrqesveeis sqrpafirpl qdlgelqegr nahfeaqltp vsdptmkvew ykdgrpitas
     2941 srittifnfg yvslnilhlr aedagsytvr avnrlgeavs saslrvyart svttdlgipe
     3001 qqryieaaee leayqqamhq kyvqeqpept sppefkspik dqnsireggf ahfearlepv
     3061 gdsdlrvewl kdgrpveass rittffnfgy valtikyvti hdvgiytcra ynrageahtt
     3121 aqlsvisknd viydsqhptg lqkiqtleds srysrqvqee tqvtqaprfl gtlkgtnkiv
     3181 egqrahfear vepqsdltmt iewyhngkai taanriqtyh dfgyvaidil qvrpedagty
     3241 tvvarnalge arlsatmvve trssvdtssm hrtsyektqr leeskfvepq yhieeisksk
     3301 pifvqplsdp kpvsegknih lecrlepmgd ptmrvewfqn grpvtvgsrf rtyydfgfva
     3361 ldivhstild sgeytvratn hlgtahtsac vrvigksdiv tetqheqsle qiqmledaar
     3421 yrktqqeevt vmqapqftrp lhnietvelt nvhlecrlqp vgdatmkvew fvngrpvktg
     3481 hrfrpsyefd yvaldilgvy pedsgvytcq arnqlgeavt scsvrvhakk dlllesqhpe
     3541 gleriqyled asrykrhelv devvkvkprf vtapknqenl hegqhahfec klepvtdsnl
     3601 kvewykngrp vtighrfrpi hdfgyvaldv idliaedsgt ytcravnlvg sdevscslsc
     3661 rstaqvltdt kneqgleqih yledrskyqr redveetttq apifttslnn veikegqrah
     3721 fecrlipvsd atmkvewfhn nkpvkagsrf ietnsfgfva ldimyayped sgtytcrakn
     3781 iigeaitsat anvhskksiy tetqneetlq rlhhledtsr yqrkttteei vtqapvftmp
     3841 ikdlkvaenq aahfearvip vgdsklkvew lrngvpiaas nrvttmhdfg yvalnmkyvn
     3901 pedsgtytcr avndlgeavt satlfvqska alqfesqhes alsklqaled tsryqrheee
     3961 eivvkekpsf tvqlngptnl vegqsahyec riepypdpnm kvewfhngkp lstghryrtt
     4021 cdfgfasldv ltvyaedsgt ytckatnklg saqssikldv ksrasiiret qhesalkkiq
     4081 yleddsrykr vdqedliiae kpkfgrplkn iehlpegksa hleatltpvn dptmvvewfr
     4141 dgrpipqghk fkttydfgyv aldilyaype dsgtymckar navgeavttc visvdskqgl
     4201 fldtldaqrl qkireletve vkqevekevv hqkpvfltpl nnldhlkege hahlecrvep
     4261 indpnlkiew fvngvavktg hrfrkthdfg yvaldilyty pedsgtymck atnlageavn
     4321 tctikvgsrr silldtqhpd glekirelea qghparleve eppvtpprfv telrgttevy
     4381 egqtahfecq ieplhdanlr ieffhngkpl psasrfhvtf dfgyvaldig htvpedagey
     4441 svrainalgq cvssielrvi prdniildsq rpegldkire leaqqpwkrp dipepqtrqr
     4501 pvftqplqni daipegqtah fecrlipvgd ptlkvewfrn eipletssri tkvhdfgyvs
     4561 ldinhvreed egvymcrasn plgeavttas mkikskasiq ldtqhpeaqr kiaqleadkp
     4621 srpeepekvf dkpiftqllt gptelwegqm aryecrvvpv gdaslrfewy ingvelkmgs
     4681 rfhvshdfgy vtldilkvit edsgvytcka inkageavss islkvkarsa idadsiqpda
     4741 wqkiqlkeae mnkvpemfvd ttpqqapvft khlesydklv egqhvyleaq vepradpnlr
     4801 vewfkngisl qtgtrlrstf dfglvtlsin glrpddsaiy tckatnllge avstcslkiv
     4861 drhwllgdtl hpdalpkida leqphvtsae qpepiyeepv fithlnnvec vegdnvhfec
     4921 nvepskdptm kiewfingkp lptgarfkst ydfgyvaldi thayeedsgv vivkatnskg
     4981 saqtsgtlkc tskqsiylqt qhpqgeagle kvkeaedayl skyrrpdegp eheypkpiwt
     5041 vplqpefklg efeplhlegq vepkddpnlk iewyfngkvl ehasrfkmts dfgfvtldlt
     5101 dvyerdsgiy tckaynkage aftsttiycs tkeniiersq hpkgkeglea iqdleeslkr
     5161 qegappesee ghppvftsqf enltnlsege iahfeasltp tgdqtmvvew fyngkvleas
     5221 hrirtvyafg mvvlevlgtk iedsgtyscr atnkwgqaei svelqcvdks kgqkpkftth
     5281 iqsleglkdg qsahfectli pvgdpqmkve wfhngqplrh ssrfkmvsdf gfvvmdiagv
     5341 mahdtgeyvc kasnkygedf tkatlkcfgk sgvyldslqp dslarirele sfggeqpttp
     5401 ttpvaeppkf itqivditrl vegqsahfea rltpvndpdl kvewyyngkk lphghryrtf
     5461 hdfgivildi lycyeensgv yecrafnkyg edttkatlkc fskasliles qlpkgmeggl
     5521 ekiqtledsm irtkdekvve ergkapmftv plsnidglre gesahfearl iptddpklkv
     5581 ewfwngkplr tgsrfrtfcd fgfvileisp iypedsgeys cratneygea vttctmkctg
     5641 krsiilesql pkgmestidk iaeleglgne pgqvtpeddt gkppefvttp sdltltensl
     5701 ahfecrltpi ndssmrvewf hngkpllags riktihdfgf vilevancyq rdsglytcka
     5761 tnrhgeatvn cklqvrgrqg iilepqlptn fktgtesiqk leealykrde ilteeetpnp
     5821 pkftvelkdi eveegapshf dcrvepvgds tmridwfhng rsfatgsrvh qindfgfisl
     5881 dmsytyards geyvcratnk wgsattkati tckskktidf dsqlpsgmsg eklkelergp
     5941 vseppveeap rqpprfithi qsatvdesep vrfecrvepk ddpnlriewy rngkmipagh
     6001 ryrtmydmgf vsmdilyvyp edsgeyvcka indlgedttr aslsckklps iilqnqvpkg
     6061 mkksealmqm eatikkytse vhlteddlyd adkkqpprfv tqildqtelv emnsskfecq
     6121 lapvgdpnmk vewffngkpl phknrftpiy dfgyvamnfg wvypedsgey lcratnlygm
     6181 detravirta gkpgiiyesq lpkgmksiek iremeaawqi vpeeegeeek vrapptfvsk
     6241 pepvtveegd csrfccrvtg hprprvmwii nghtvvngsr ykltydgmyh ldipktrqyd
     6301 hgkveviars svgesrtett ltvkprsddy rgvlknsprp wydygltqyq terqntelek
     6361 vfderhhnvs qgieiatehl gqkvfkepet dwqksvkskk neeyynklmt leeeqvlket
     6421 rlresshqfa ipgekvvads vakgmaqkye etleekkdek tqetvqpppr plkkphttev
     6481 didvermkvt gkyppgpses tvhgrevhva kqkqtqkevk gdleitrkit atettevehk
     6541 aktqervvqg ptkpakppvf tkkiqpcraf eqeqarfeve fdgdplptvk wyredfpiqn
     6601 sadlqiytfs tksvlivrqv fmedsgvfsv iaenrggkak csanlvveer rrqpgrggvi
     6661 ppsflstiqn tsvttgqlar fdakvtgtkp ldvywlkngk kitadirykt leedntytll
     6721 lletvpedsg kyecvainka gearcdaect vrgpqspaks akpttpgvek apavleplkd
     6781 qtiregtsva fscrisgkpv ptvqwkkgdk vikpskyfqm skegdfctlr iseafpedeg
     6841 vykciaknpa gdvttsanlr vlapdaadvl pkltplkdqi vsegqpaqfk tqvtpskpkp
     6901 tiqwyregal ipqspdfqmi hegnnavlli attyeedtgt ftcrattsag tvetsakliv
     6961 kkrtgayyev paetgpgart gdkpfvslgt gkdvilepip ldkgeleipl kpgdetlpwr
     7021 kgrvhhrprl adttpwrktk pksrdtsldk lqatesqikp wteetvtlkk tpqqareipk
     7081 ekleevelkp akiekkeirr psletvdlkp ftkettketk ieekltrree idkeiyeeee
     7141 dsrflktsrk vdetvqrkkd evkpwteekv tlkkskpekk elpketletv elkpskigkg
     7201 evrkpslekv dlkpvpttvt ekvsekvtek teyleqedts lldvsrteev kkkrkekvee
     7261 kpekpeqakp wteekitlkk skpvvkeiek etletvelkp skigkaelrk pslekvdlkp
     7321 vpttvtekvt ekitekteyl eqedtslldv srteevkkmr kekveekpeq akpwteekit
     7381 lkkakhlpke ieketvetve lkpskitkse irkssvekvd lktvtketke itqktdyree
     7441 edttllevtk dtqvkktrre sipvekvegp kpwteekvtl kkakpekkev pkekpetvel
     7501 kpsrpqkadi ekpsledvdl kpipkkvtke ktekdkyiee edtsllevtk dtetttkvek
     7561 kvkrreevek kpetkpwtae kitlkkakve qkeirresie evtlkptkte krqvtketle
     7621 kvdlqkvtdk tqfveeedtt lmkvdkvekv eeervdkmat wrrdrkpkek apyheeedks
     7681 ildvkrdees tekrkevpvp wargkkkpte kapyheeedt slldvkkdee siekqkevpv
     7741 pwargkkkpk eekpelvpqq vqpeekpeee etlektqvpw lrdkkvarki perkeepved
     7801 iksvtlkpvr rerapeekek peevtlkpvk ripreeeeke evqlkpipkr kpeekpqeei
     7861 ilkpfqkpkp eveeqekpkl kpfekakpev peqekvqlkp fkrekpevpe kekvelkpfk
     7921 rekpeetlvp qkvpekpeee qpseveevsw rrkpkkkeai ipekpvekve evkvvedkvi
     7981 kkqeetiedf ksftlkptkr gkkleekeek eevtlkpvkr lpkeeetkae iklkpvekkv
     8041 eekeakplkp emaptvpeee kptevsevpw krgkkkpvek rveevtteek kveeeekevs
     8101 elswrkpqke kvkevkkeqe vedftavmlk ptrkekkpee keekeeitlk piqkvqkeee
     8161 kpeavtlkpv kvekpeavek eevpeektel pwrrgkkpak kivpqeevkp eeigkpeerv
     8221 pevkeevpkk pkvekpeeek levklkpvkr lekpeeakpe evtlkpvkrl pkeeeekeev
     8281 klkpikrekp eeekpevklk pfkrlekpee ekpeevtlkp vkrlpkeeee keevklkpid
     8341 rkkpeeekpe vklkpikrle kpeeekpeev alkpvkrlpk eeeekeeikl kpikrekpee
     8401 ekpevklkpf krlekpeeek peeitlkpvk rrpkeeeeke evklkpikke kpeeekpeik
     8461 lkpikrlekp eeekpeevtl kpvkrlpkee eekeevklkp ikkekpeeek pevklkpfkr
     8521 lekpeeekpe eatlkpvkrl pkeeeekeev klkpikkekp eeekpevklk pvrrlekpee
     8581 ekpeevtlkp vkrlpkeeed keevklkpik kekpeeekpe vklkpvkrlp keeeekeevk
     8641 lkpikkekpe eekpevklkp vkrlekpeee kpeevtlkpv krlpkeeeek eevklkpirk
     8701 ekpeeekpee iklkpvkrld kpeeakpeev tlkpvkrlpk eeeereevkl kpvkkekpee
     8761 ekpeeiklkp vkrlekpeet kpeevslkpv pkvvpekdee kpeeeapets ilpwrrgkkt
     8821 kkvvdfheev tvvpidqkki vsevhekett iveqkveake viekkvvvee vkpevveevt
     8881 evsevswrkp rkpkkpveek evvelkpvek heeivekvee kitlkkvkke vpeveekkei
     8941 qlkpvpkkpk eeevpekitl kpvkkekpee kpkeeeipel kpvklekpek veeteekeev
     9001 mveitrkkpk kkpeevpvek eevveteite iteekpeeis vlpwrrpkkv tekkieveei
     9061 kpekvedkev eevtettevs wrkqrkpkkp ieeeekpekv tlkpvkrekp eekpkeeipe
     9121 lkpiklekpe kveeteekee vkvevtrrrp kkkpeeiqre eievetkevv ddvevekiek
     9181 qeiqiedrkr krkqrtqvdi siidkdkkia prfiqklqpv iaepestakf tctvfgspvp
     9241 eiswyrneqe lhasekyimt vyettaslei tkvkeedagm yscrasnpag vatstvnlvi
     9301 fekeeegiap hfstpikplm veehkpalle cvvtgspvpe vkwyrgeqel kpkkgreitf
     9361 npetgeaklh ilepteedet iyrvravnkf graecranll innvvkvskp evlrapkitr
     9421 plpalvaerg kpltlsadfe skptpevkwf rngvelapse knviniyent aelfipevtk
     9481 kdggkyeirv qnpvgearss gsvtvkdrpd ktdevkaprf ieplqpqiva egevvimetk
     9541 vesyptasfq wfhetrples tpqvrivtqe nksilmikei kseyagtytc raenvggsvt
     9601 ctatinllet pweetvelvs ptfvkrlspv rvmdgesanl tcvvqgkptp kvewyhdskp
     9661 ikegkeitiv qdtegvcsla itevfpedag eytcravnpv geavcttsli veayeyvpds
     9721 eiassivats lttgqsgsee dllspketpl fdtdteesap eiikklpqli ptkdgeltrl
     9781 evkvkgkpkp egkwykqgve ivssqefqie efedgtsvlt iaetypddtg eivfeahnpl
     9841 gvsttmtyls vegivgtkey rkpewvthme emqealratq tvprfiqeit dvyvkegdta
     9901 vfecsysgnp tpdvvwyknd kmlmstqnlk vriydddkkt vltikhatee ddgayvckit
     9961 setglatska klhvtdvtde kkfyeeeeev eeqtievkpk ekpqkkklrk akevkekvkt
    10021 ervkvdkkev rkeklvpkeq peekkivdie etqilestdi ieekptevsq akkivpiqep
    10081 vlteataslk kiddekpkdm vpkpeerakr vieeregivv vsevttedma pdftveskvd
    10141 raqitseslq tvtvsevhte tsvqevrrae akqrkakktv vteeeifeer pkevvveekp
    10201 kkkkktekvk vreevtieei verqreniar eveeimemlh akefgpgeap lrelatigyl
    10261 vrqgvsvnei neslyrtenf palktpeaqn alvqlvereg hgplitqvlt eetttdesvv
    10321 aatvgfrafm rmielqhvtv eevlthfape afrprawevt eateveteer vtervdvvrk
    10381 tevhfmerrd erkavrtqdi rdikeyedtr qthttlrark drkpreetke drdegvqvve
    10441 ieeiedteqe kmkkkrkdve eieeeietvi vetdskgrla qkkkkrddle ekedveeeee
    10501 iiekvrkelk pkrpkigrde veleeieefd vtedksmpsi mvnvpsqrhq vvpldrtteq
    10561 apgkpeleka kltvdtital tehivpvqek eisdisrikp ierkaslsis pvepysttet
    10621 tvqastgefa dtfkptsyea tpgiipsegv qvsetlvsda gvtslsvskq eigrtaevsv
    10681 tlqeattvye tmvrekevpt edfvtpqtvk aedtilpqls lsvyevhegl tedkleplkt
    10741 vatkprvnvt aveplvveev raedkpgkyy pelivpteva tqsvisqrqr vteemhapek
    10801 egeyipgrlp psqvaqigis ygnetavvqq nvvqesegvf isekktetfe attnvtvleg
    10861 vsvstvqtqd tetsltieet kkavadlnvi emtpavtvet vpsekekeyq pgdkpdtkta
    10921 etsissleig siqstvvqes egvyrpdekp tkalaetsir peehvmisei qtadypsdfr
    10981 eelkyvqesg tvsvqlteak miqetlthdr eermeemvkp eermvdtsyd vnrsvevfqt
    11041 tsvekeadlr vfempeshrg ktvpthpvvs levemtqped nlgevkkevp ssgtakvehv
    11101 slqetivget vvaenlapve kdkapetkva eiamnevesl httmvvvsek eteytqvsdv
    11161 kgayasteft tqvapmleev rshspteeyl pdqkptsgia qpshvplesi tvavqelaek
    11221 edlyktdvrp dqktanvalt eltsisipev mqvitassae tlkketkmke ekgrveveel
    11281 sstiisevis netedvfttk atpkdqkadf nisgreaaet tqvtamteae dlavkrpeeq
    11341 kgkpqvdelt pltvshvetg eaekelptae vpkektaqpn ifgrdvaetm qvltvtsaee
    11401 laapkapegq kgkpqveelt plsvsqvvsq eaesalpape apterkaqpd mtgrdiaetm
    11461 evltmtsadi laedvkpteq kgmpgleefv plsvsqtvst ekedvlpspe apttktaqps
    11521 ltgrdiaetm qvvmmtseke litalapeth kgipnveelt pltvsqtvsh eaedvfekta
    11581 apsevtakpa fsgrevaqts emmivtnvge lekqvlpdeq kgqaqldelm pltvsqvisg
    11641 eveeglpeae kpkektaqpg ilgrdiaett qvltvtsaee laapkapegq kgkpqveela
    11701 pltvsqvvse eaegilpape apterkaqpd mtgrdiaqtm evltmtsvdt ftedkkpdeq
    11761 kghpeleefv plsvtqtvsn emedvlpspe apstkiaqps ltgrdvaetm qiltmtseee
    11821 litaqtpeqh kgkpnveelt pltvsqtvsq eaeeilekaa aptevtakpa fsgrevaqts
    11881 eimivtnvge lekqvlpdeq kgqpqldelt pltisqiisg eveeglpeae kpkektaqpg
    11941 ilgrdiaett qvltvtsaee leapkapegq kgkpqveela pltvsqvvsq eaegilpvpe
    12001 apterkaqad ltgrdvaemm evltmasvdt ltedkkpaeq kgvpgleefv plsvsqtvst
    12061 ekeevlpspe apttkvaeas ltgrdiaetm qvvtmtseee lisarvpeqh kgifnveelt
    12121 sltvsqtish eteeilekaa algevtakpa ftgrevaqts etlivtnvge lekqilpeeq
    12181 kgkprvdemt sitvsqvvsg eveerlpeae kpkektaqps mlgrdiaett qvltmtsaee
    12241 laapkapeeq kgkpqveela pltvsqvvsq eaegvlpvpe apterqaqpd ltgrdvaqit
    12301 vvmsmasadv middkkpeeq kgvpgveefv plsvsqtvst ekedvlpspe apttkmaqps
    12361 ltgrdiaetm qvvtmtseee litalapeth kgkpnveelt sltvsqtisq etevileksa
    12421 apseatakpa ftgreiaqts eilavlnvge lekhvlpeeq kgkpkleelt pltvsqiisg
    12481 eaeeglptae vpkektaeps mlgrdiaett qvltmasaee lmapkapeeq kgkpqveela
    12541 pltisqvvsq eaegvlpvpe apterkaqpd ltgrdvaqtt vvmsmanvdi mieekkpeeq
    12601 rgipgleefv plsvsqtvst ekedvlpspe vpttkmaqps ltghdvaetl qvltmvseeq
    12661 lttkapeeqk gkpnveelts ltvsqtvshe aedilekava pseatakpaf lgrevaetsq
    12721 vltvttvgel qkdvlpeeqk gkpqleelmp ltvsqivsge aeerlpeaek pkektaepsm
    12781 lgrdiaettq vltmasaeel lapkvpeeqk gkpqveelap ltvsqvvsqe aegtlpipea
    12841 ptertaqpdl tgrdvaetme vltmasldtl iedkkpaaqt gapgleefip lsvsqmvste
    12901 kedvlpspev ptekmaqpsl igrdvaetmq vltmisamel ttghapdqqk gvpnveelts
    12961 ltvsqtvshe teeilekava pstvtakaal sgrevaetsq vltvsnveef eksilpegqk
    13021 gkprldelts itvsqvlsne leetlpspek psekiaqpqm igrevaeqte vltvtnveel
    13081 kklekpegqt gkpdveemsf itvsevfste mekempspev pkertalpql ssievaette
    13141 vltmisaeel lkskapeeqk vqpkldeltp ltvshvvsee aeealptaei pkektaqpsl
    13201 lgrdvaetsq vltmasaeel laakvpeeqk gkpqieelap ltvsqvvsqe aeealpsaev
    13261 pvereaqpsm tsrdvaqtml vtsmsnvdim aeskipeeqk gvpgleefvs lsvsqtvste
    13321 kedvlpspev ptekmaqpsl igrdvaettq iltmisatel ttdrapeqqk gvpnveelts
    13381 ltvsqavshe teeileklea pgtvtakaal sgrevaetsq vltvsnveef eksvlpeeqk
    13441 gkprldelts itvsqvvsne leetlpspek psekvaqpqm igrevaekte vltvsnveem
    13501 qklekpegqk gipdveemsf itvsevvste aekelpapea pkerkalpql sgievaekse
    13561 vltmtmteef akpkapeeqk gkpqleells lsvsevalge vekglptpdk pteetakpsl
    13621 lgiqvaeksq viassitgel qesikpdtkq iipeqipfes ieqsqtltqe restfvvekt
    13681 ttsataevsf rvseslevtq vtatekeske vikgvakeas aqadvierkv alktevqped
    13741 masefkvkkp egktakgvde iqqgvivteh ltahefesel pesvlpfakl arvsiqadhl
    13801 ehivetteap aqqhhititq httkhdlpqq qsiesdtevi eeyttkfrrg stedataavk
    13861 tkktivrkkp kttgeedivv vieeelddik pkipsipkkq fmpieevttv sdveeivsar
    13921 ideaeeivpk eeekvevtit evqdtdkkkk vtkkqfvrrr gktrqvteei tveeegkape
    13981 ttvvtkpvei ieedieerpk iekeeyvvee vpeektiplk prkkltptvq qveeqvtvte
    14041 eitkegkprk ttkkkvitrk gkeqqvtevv tveeqgkapi tsvtegpvee vveeiiqklp
    14101 apkyakpsev eevreqvtvt eevtrlgkpk kttkkkiikr kgkeqqvtev vtveeegkap
    14161 tttvtegpve evvieeikel pipefvkpte veevreqvtv teeltkmgkp kktikkkiik
    14221 rkgkeqqvte vvtveeqgka pvttvkegpv eevveetitp llapeyakps eveevreqvt
    14281 vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp veeiieeeik
    14341 pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv
    14401 ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    14461 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    14521 tregkpkkti kkkiikkkgk eeqvtevvtv eeqgkapvtt vnegpveevv detvkalpap
    14581 eyaepsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    14641 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    14701 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    14761 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    14821 reveevreqv tvtqevtreg kpkktikkki ikkkgkeqqv telvtveeqg kapvttvteg
    14881 pveevvdetv kalpapeyak pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    14941 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    15001 kktikkkiik kkgkeqqvte vvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    15061 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvmegp
    15121 veevvieeiq elpipefvkp teveevreqv tvteevtrlg kpkkttkkki ikrkgkeqqv
    15181 teivtveeqg kapvttvteg pveevvdeti kslpapeyak pseveevreq vtvtqevtkl
    15241 gkpkkttkkk ivkrkgkeqq vteivtveee gkapkttvte gpveevveee vkpllapefv
    15301 kpseveevhe qvtvtqevtr egkpmktikk kiikkkgkaq qvtevvtvee egkvpvttvk
    15361 egpveevvde tvkalpapky akpseveevr eqvtvtqeit klgkpkkttk rkvikrkgke
    15421 qqvtevvtve eegkapettv tegpveevle eeikplpgpe fvkpseveev reqvtvteev
    15481 tregkpkkti kkkiikkkgk eqqvteivtv eeqgkapvts vtegpveevv detikqlpap
    15541 kyakpsevee vreqvtvtqe mtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    15601 tmtegpveev veeeikplpa lefvkpseve evreqvtvte evtregkpkk tikkkiikkk
    15661 gkeqqitevv tveeqgkapv ttvtkapvee ivdetikelp alefakpsev eevreqvtvt
    15721 kevtkegkpk ktikrkvikk kgkeqqvtei vtveeegkap kttitegpve evveeeikpl
    15781 palefvkpse veevreqvtv teevtregkp kktikkkiik kkgkeqqvte vvtveeqgka
    15841 pvttvqegpv eevidetiks lpapeyakps eveeireqvt vteeltklgk pkkttkkkit
    15901 krkgpeqqit eivtveeegk apvttvtegp veevvkevlk plpvpkvvkp seveeireqv
    15961 tvteemtkeg kpkkiikkkv ikrkgreqtv qevvtveekg kapvttvtvg pteeiveetv
    16021 ktlpapeyak pseveevreq vtvtqevtke gkpkkttkkk vikrkgkeqq vtevvtveeq
    16081 gkapvttvte gpveevveel lkpliapeyv kpseveevre qvtvteeltk egkpkkvikk
    16141 kvikrrgreq qvkevvtvee qgkapvttvt egpteeivee tikalpaped akpseveevr
    16201 eqvtvtqevt kegkpkktik kkvtkrkgke qqvtevvtve ekgkapvttv tegpveeive
    16261 etvkalpape lvkptevtev peeltvtevv tkegktkkiv kkkvpkpkke gieapeeape
    16321 evpveetkki iklqrlkvsk festkldiqe tslgkpqfaq iklrktpvak rpskkdekfp
    16381 rillrsriia iewppspknl kvdtfepiel qngilsrnvd eaaalpkpkk kkikriekei
    16441 aklekldqef eelkkelple kleepileek perkvkkkkk pedkakegvk pqlmrisike
    16501 qkpikpkiae patplfaqik lkkapakpkk kpeeevskfp killrsrita hewppsvkyp
    16561 aisvlepnyi qngilsrtme ealklkkvkh kkmklpekel telekldqef eelkkvplek
    16621 veeitpyerk pkpekpeeek pkklvigkgk rrpaeeedle tvqlkkipek ipsapeeaek
    16681 vpkkkdevdk pkkekeekey pelkpfepyd ieapevelee rekpeypepe kpkekkvpgk
    16741 kpkkerktpa qeteavpivp gvpkpkepee eeeikrripe rpppteepek iklkpwkkge
    16801 kpeeeekkpe dyikfvpkdd divetvevtt tveetpdkkq rkikkkktit kpkdekpkii
    16861 eettieeegf epvvtveefd vdkyeavpve svekaapvee ikeeivtsei iteegtkktv
    16921 rkkrvtkkre gkervteevt veeegkepit atielhedti seiheqakhk rptkpkadek
    16981 laedvtektv eqqeiieevk evkptkkvik kekpvkakde vteevveeii ieepekqeak
    17041 piveeeetvi itehdvrkap kkkkevvike eeeifetiet pthkiirkki rpmkkgekev
    17101 vveeiiqkpr eetvemeeqq ivevvetptk kivkkkkevv kddgvpeelv eeiiiekpqk
    17161 kkakptveee kkkvkvtkvd tkkapekpee vveeeeeiie tietptkkii rkkpkdkkdk
    17221 iieeviqkti eeakameeqe ivevietptk kvikkkttti eegkapeekv eeiviekpke
    17281 vieeeeeiie tietptkriv rkkpkdkkdk iveeviqkti eeaktmkeqe iievietptk
    17341 tvikkkkati kegkapeeev eeiviekpik ekakkkilpk egveitevit etsveeliee
    17401 elkpeeakps eeveeekvki tevaikkvpk kkkpskavea eekeevveei kpekpkeeka
    17461 kptvtekegi evteviseva peklpeeakp kkkkateike teekvitkkv kvkeapkeav
    17521 pgeeeqpaee vdelvpekpk ktkakkevtp qdsivttevv pitkeeelev ekpeeekatv
    17581 veekeekakv eekkvtvkka pkkklkptea eeeqveevve eitpeepkkr kakkavipke
    17641 eiqttevipg itaeeipeee kpeeekavpk eeekveeeak vtevsvkkke rkkkekpvtn
    17701 gevieekpkv keipeekpke evpqvpeeke kpeeekpkev kpkekkpkee kpkkpieekp
    17761 eevkpeeekp evkpeeekpk eekpkkkkpa ekkpkiseee rpedkveeip ekkpeevktr
    17821 eeiipkdtvq ttevipetva eeiptkkpke rkavpveeke ekveitevpv ekapekkeek
    17881 vpeekpteaa pekpkeakvm kkkkkkpakk deieewvepe yerpvlepmp ekivwepkkk
    17941 kekkplpesl qklvpqkier kemkptklky sepaetvqfa siklkkvavp kkkeadetkf
    18001 pkimlrsrmt fvgdyppelq ipkityleen pvqtgilsrn teealkvlkr kvkkvklsek
    18061 evtqlekldl efeelkkvpl ekiedksvye rqpkkpaekp eepkkvpigk gkvpeeekve
    18121 pekvklkkvp ekkpeeveep lkkpkkpepe eevkpekkkk pkvklehdel kpldfdrpel
    18181 ekyepeeyek peepapepvp kpyekepkkk pdqeveqipl kkgkpkspep deepevkfri
    18241 ptkekpeeep eqitlkpwkk ekpeeekeke ipkkeeekvp kppkeepedv tvqrkaktkk
    18301 pkekpkeeke eevtvkkpee ekpeevaeev tlkkepekpv eeapaevtik kpvveekeve
    18361 eeetitevil kkkpekpeev veeavvkkpk kkkpvkeeev veevtvtkvv teeekeeekv
    18421 vikkpkkksi peiteevtee itlkkpeeka peevteeikk pkkkkpvkee vaeeitikkp
    18481 vveekkeeev peevtlkpkk peekpvieev teeitlkkpv peevteevai kkpkkkkpve
    18541 eeaadeitlk kpvvvekeee eiteevtlqk kkkpekkpvp eeiseeitlk kpvpkeeeka
    18601 peevteeiai kkpkkkkpvk eeaadevtik kpvveekkee etpeevtlkp rkpkekpvpe
    18661 evkeevtlkk pvpeekekvp eevteeiaik kpkkkkpvee eaadevtikk pvvvekeeee
    18721 iteeitlgkk kkpekkpvpe eiteeitlkk pvpkeeekap eevtekievk kpkkkkpvee
    18781 eaadevtikk pvvvekeeee apaeitlkrr pkekpveeit eevteeitik kptpeekeka
    18841 peevsekvev kkpkkkkpvv eeaadevtik kvvveekkee eapeevtlkp rpkkksvtee
    18901 evaevtltkp kpieeekkpe eseltikkkp kkkpvieeea aevtikkpvp vepdeapeei
    18961 tirkkkkpek kpkvteevee tavtikkara pekpteeeei ttdvqikkkk pqkkveevaa
    19021 deltikkiee veqpeeeeev qeftfkrkpp klppkpieei yedvtlrklr pkrksrpdik
    19081 eitetenvtf rprttktked veqefkisln tyeeedisms gkvrlkpkkk pltyseetge
    19141 etiriiqeve ddsgpiieei iesdeegkde ysieeldtde mrlplrrkkk kvpkpykved
    19201 ieegdvklkl krerkysiee adeslalklk skrrvstyde eeatlsitre ediseeveie
    19261 yvvrdgdtmf sicsyvaetd eainlveger vyvidhtnqd wwfvkkhlte ekgwvpaqyl
    19321 lnevhytlyl qrklhekidk lpvfekpapg dkssapkfve klqpihtpdg ytiqfecqve
    19381 gvprpqitwf rqtaiikpsp dfqmyydddn vatliirevf pedagtftcv aknaagfass
    19441 tteliveapl sdhgsdltgp srkslsress ladilegipp tfsrkpkaky vdegedvile
    19501 crlvavpepe itwyykdtqi ttkeniiiat esdmhmycsv ikitkvqkkq egkytivarn
    19561 regeatieip mkvktgksep peileplqsy vvregetvvl stqivgnpap kvtwfkngkp
    19621 lkgltpkqdg hvntltliqp qvsdtgeysv vatndlgtae tkatltvein lkpslainfq
    19681 qhtfehfsvt keipsgapep plfterfqel tvpekgtfkl vakvtgnpvp evtwlrnnkp
    19741 lekspnitet ydgenivlei rnansevdtg dykcvasnpv gkashgarvt vdvdkvtftk
    19801 tlekevvvde yktleltcet shtvstkwwh ndkeisgmdh reiieegrvh klvikktnpt
    19861 degtykctvk nqstssnvtv katkpefvkk lqdcevkerd vaileveits qtvdvkwykd
    19921 geqlgpgkek lefvkegtvr kllirstsvh degeytcsll dqectaevtv ielppeiitk
    19981 mqdvtiarge ratfdieltk gdalvrwfkd gqelqfsehv rlsidgkrqk lkiydtesed
    20041 agvyscevgq qkssakltve epavefitkl pdvtlvplns davfvielsq dvpvtwmrkt
    20101 qvikespkyt iidegkvkkl ivkkcttedi seytamvanv ktssrlrvev ietppkispd
    20161 tprkykvrkd edvdivvkfs gtpkpedewt vngrvvtksk raiptidees avltirkiqe
    20221 edvgdytlkl vnsvgetsie invvivqvps apgapeplet tndsvtlhwk kpdsdgnspi
    20281 veyilehqek tettwtkite titetthkvt klttnkeytf rvtavneagp getsptspyv
    20341 kiskpstaep pvileplksi viglgetvtl scviggtpap eitwlrndei fedtsityen
    20401 hitkytiret tetssakftv kaknnvgsae ttcelkiqes pkiiydenlt nqklpmngqw
    20461 kieiqitgfp kpevtwtkng kevdekrvsi hreertstvs isslvredta tymakaenqa
    20521 gsssvdlhlk vidkpskpqg pvvfkeirqd rvtlewrppa ddggieleky tiekcepgkt
    20581 wvkvvdidke vesfcvhklq qnaeykfrii arnavgasep lesdtvkmrt sfeppgaprg
    20641 plevsgmtkt sftikwqppe ndggtpitdy iieikeeskk twqkigstkh etthfsvsdl
    20701 ktdvpynfri taknsvgsgp pyvaeepiap grrikitklt essmyallgi fpptkivisk
    20761 tppsspqhvh vtnitsksat lswsppastg gteltgyvie krpligkgar wtkvvtldat
    20821 tlqycvenlk eseflfrifa ennqglsvpa nsepvtlkth asvpspptap leirqiaant
    20881 vviswgrpds dggaplegyk vairdtkrtm wmevarvnte iqklnirdlq enhmylirif
    20941 arneiglsdp leseepfkii paseltvvep iaeatekget asisfstent sswlrehnmd
    21001 adihsyarar llrqdeyffr iwhyakklfe
//
LOCUS       XP_034182893           21030 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X14 [Osmia lignaria lignaria].
ACCESSION   XP_034182893
VERSION     XP_034182893.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327002.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182893.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21030
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..21030
                     /product="sallimus isoform X14"
                     /calculated_mol_wt=2376500
     Region          140..229
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          157..161
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          170..174
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          192..199
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          209..214
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          222..225
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          236..326
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          253..257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          266..270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          292..296
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          306..311
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <961..1232
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          1947..2036
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1964..1968
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1977..1981
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2002..2006
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2016..2021
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2029..2032
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2193..2280
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2210..2214
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2223..2227
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2250..2254
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2264..2269
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2390..2479
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2407..2411
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2420..2424
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2445..2449
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2459..2464
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2472..2475
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2515..2605
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2532..2536
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2545..2549
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2571..2575
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2585..2590
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2598..2601
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2614..2704
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2631..2635
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2644..2648
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2670..2674
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2684..2689
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2697..2700
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2761..2852
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2778..2782
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2793..2797
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2819..2822
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2832..2837
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2845..2848
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2894..2986
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2912..2916
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2927..2931
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2966..2971
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2979..2982
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3033..3125
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3051..3055
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3066..3070
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3091..3095
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3105..3110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3118..3121
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3167..3259
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3185..3189
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3200..3204
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3226..3230
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3239..3244
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3252..3255
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3301..3393
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3319..3323
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3334..3338
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3359..3363
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3373..3378
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3386..3389
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3435..3526
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3452..3456
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3467..3471
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3493..3496
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3506..3511
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3519..3522
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3568..3658
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3586..3590
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3601..3605
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3626..3630
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3640..3645
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3653..3656
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3702..3793
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3719..3723
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3730..3738
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3759..3763
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3773..3778
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3786..3789
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3835..3926
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3852..3856
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3867..3871
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3892..3896
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3906..3911
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3919..3922
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3968..4060
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3986..3990
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4001..4005
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4026..4030
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4040..4045
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4053..4056
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4102..4194
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4120..4124
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4133..4139
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4160..4164
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4174..4179
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4187..4190
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4234..4326
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4252..4256
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4265..4271
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4292..4296
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4306..4311
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4319..4322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4367..4459
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4385..4389
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4398..4404
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4425..4429
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4439..4444
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4452..4455
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4501..4593
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4519..4523
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4534..4538
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4559..4563
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4573..4578
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4586..4589
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4633..4725
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4666..4670
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4691..4695
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4705..4710
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4767..4859
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4785..4789
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4800..4804
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4825..4829
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4839..4844
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4852..4855
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4899..4989
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4916..4920
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4931..4935
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4956..4960
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4970..4975
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4983..4986
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <5066..5126
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5070..5074
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5095..5099
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5109..5114
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5123..5126
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5174..5265
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5207..5211
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5232..5236
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5246..5251
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5275..5366
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5293..5297
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5308..5312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5330..5337
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5347..5352
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5360..5363
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5408..5499
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5426..5430
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5441..5445
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5463..5467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5480..5485
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5493..5496
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5546..5637
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5579..5583
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5604..5608
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5618..5623
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5684..5775
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5701..5705
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5716..5720
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5741..5745
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5755..5760
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5768..5771
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5821..5911
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5838..5842
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5853..5857
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5878..5882
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5892..5897
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5905..5908
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5954..6043
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5971..5975
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5986..5990
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6011..6015
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6025..6030
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6038..6041
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6097..6187
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6115..6119
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6130..6134
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6152..6156
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6169..6174
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6182..6185
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6235..6323
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6252..6256
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6265..6269
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6289..6293
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6303..6308
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6316..6319
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6558..6648
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6575..6579
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6588..6592
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6613..6617
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6627..6632
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6640..6643
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6662..6751
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6679..6683
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6692..6696
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6717..6721
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6731..6736
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6744..6747
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6772..6861
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6789..6793
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6802..6806
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6827..6831
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6841..6846
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6854..6857
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6960
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6887..6891
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6901..6905
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6926..6930
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6940..6945
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6953..6956
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <7037..7734
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          7544..>7953
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          9211..9300
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9228..9232
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9241..9245
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9266..9270
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9280..9285
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9310..9401
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9327..9331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9340..9344
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9367..9371
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9381..9386
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9394..9397
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9416..9505
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9433..9437
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9446..9450
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9471..9475
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9485..9490
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9500..9503
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9518..9604
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9535..9539
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9548..9552
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9573..9577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9587..9592
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9600..9603
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9621..9711
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9638..9642
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9677..9681
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9691..9696
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9704..9707
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9759..9845
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9778..9782
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9791..9795
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9817..9821
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9831..9836
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9883..9974
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9900..9904
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9913..9917
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9940..9944
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9954..9959
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9967..9970
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <13462..>13614
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          19268..19320
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(19273,19275,19281..19282,19299..19301,19314,19316,
                     19318..19319)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          19356..19446
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19373..19377
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19386..19390
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19412..19416
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19426..19431
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19439..19442
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19480..19573
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19497..19501
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19510..19514
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19535..19543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19553..19558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19566..19569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19581..19667
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19598..19602
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19611..19615
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19633..19637
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19647..19652
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19660..19663
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19701..19791
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19718..19722
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19731..19735
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19756..19760
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19771..19776
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19784..19787
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19804..19881
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          19814..19818
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19825..19829
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19850..19854
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19864..19869
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19885..19970
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19902..19906
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19914..19918
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19940..19944
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19954..19959
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19963..19966
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19975..20059
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19992..19996
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20004..20008
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20029..20033
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20043..20048
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20052..20055
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20165..20245
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20173..20177
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20186..20190
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20211..20215
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20225..20230
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20238..20241
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20249..20340
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20249,20314,20329)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(20330..20331,20333..20334)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          20351..20437
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20368..20372
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20381..20385
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20403..20407
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20417..20422
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20430..20433
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20461..20531
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20461..20464
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20473..20477
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20497..20501
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20511..20516
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20524..20527
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20535..20627
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20535,20601,20616)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <20605..20920
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(20617..20618,20620..20621)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..21030
                     /gene="sls"
                     /coded_by="XM_034327002.2:253..63345"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mhassssdkh fsaekseigg enkvltkmse titesstthk tlkkeyisss mssstgapgq
       61 eptsvcvkst vhsteqsssa dgappvvqsy kveehekiiq dqpgeirqer tvvvsqdeeg
      121 ikrdikatqi pkpvrkstap rfvspvtgmi vdqgsdivle giidgfpqpv viwskngqel
      181 ktkdsmtity shnhvrlelk nvnvkdagry tctasnevgs asstadlvvk ktifppvfgr
      241 rlqaqlvkrg drvnmeveit gtpeptvswf kddipikerp pelrikqqgn cymlimekaa
      301 kehagkymvr atnaggeaqs iadfavfept pdtmvevhkt viydvqdkkl mqsdekkaev
      361 psskhltseq vsttkiqpaa tikttptams tsmaqmsrtv ekikepdtel crsekisstv
      421 eshqmerkse qkyhmklehk mpsivepkte gktvikevir eevrssepks tlieekiene
      481 nietsriakk dalsffesmt retetipkgp kemiklvdda dghevkvgkl tknyekstsf
      541 qevkkpevkp pellttkkav heiftkleqg sipsrgidnk lfdfpyepyk ptmetkktvv
      601 eetissgsss lqssmtmskm eskreskmes kssellsegf nlvpepppei gympkpeevk
      661 kkrpevsika kqlqesfdkt lspidapigg vkifpspspk iletpkvapp sfsmppppfq
      721 lekkeiveev cikkdvhekr dpqplvrpis psrpwssssd vetrsqmstd lseyrchsaa
      781 sshqeilrss sprpsadgla mekswakkca dstrkswppq edtpikykqe wqmpgedykt
      841 sskefkreve etpeggikkt smessstter rswsskqetm dkmskrapsp pkiqpiiyna
      901 etikvdhtvn kieekslyek yisecdvkks qksekleeys skkwtgaedl kapglvrsle
      961 tpkkptpply rpvepiptdq iilepgpppe igyipapvvr ekkieqiekt levslehkpa
     1021 kippggirti ppapkkeepp alppkdvrit ppplpakqpt pveplepfpf kpspsptikt
     1081 pkvavpaptp skfvkgsfgv esdyesdmds hmkakwrpye sdneepryrr vqppvpkqpr
     1141 prstepeplp pskfevpses tglprpvltk eqqekmykkt ttfkrqeket kqqkyqvvqp
     1201 ppvelkpgsp piyvqpatks ptpksppakk pespkfkvkt fqqesgymad tdeplqqkss
     1261 vaslsksfgk qessssmssh mesrtsyses rseyfesksy dsrqqqqkke sfapsapssv
     1321 kqppqqrssf veksygssav tssprfmsak etlyttdqsh kkfeapkkil ppspspsrfv
     1381 ksefresdye sdydgrippl wkprgyesdd qsfrtvkpnl agpgkpkvqr edtsrlieea
     1441 straqkaktf ekreervatk pktvllpgsp peiayappqp qptyyearsg ipfhnaigte
     1501 tkktvrmdes tensrrivtv eqtsrvikfg dnqksatdfd sfdsssaqkq kthykvptpk
     1561 kfvqgqfres dyesdidtri kpkwappesd seepryrkvq ppttkttrss sapvrqehvv
     1621 spmefdtgpt vlrkqtsmsq lrderkfdgq vkrqassrqe etlkpgsppe fgyisrsdvk
     1681 kaanhvasrh msdmtssfks ktekfvtdiq sdlkqgkpil kqpvdgrasd adeprtyree
     1741 srlsehgtkq idpdtgliyf kydfgyefgi vlpgegkktv sstsrsvqgq rrasdievpi
     1801 vhefttrken gfakrsasss starqgkpag kfsgsktvkw eptseseyse sedirnarkr
     1861 hseggntmta pslvipcsps psrwdhttps plslspslps lspryssatg ppsnvdsags
     1921 pwpttngvas skeviqpyle ilpkkaplfi tplrdiavvs gqtarfeciv qaepqpnilw
     1981 skdgriiens tsyeihyrng vcrltivraf pedagtyact atnslgstvt satllvpgvp
     2041 spfygtyrdq nlsersaesp pkyvalrdlf lpqesdvkta ssnrqqqhns stfeyawsvn
     2101 rsyaggryre kenateecve itsrnpiarr leysdfqand sfrdvadfcf dreystcnps
     2161 iasprntvqg eivmqttptv paftgrpgdp sppvfeqifk narfaqggna ifegrvrgnp
     2221 kptvtwthkg aplleswkir msyddktgav tlqinqigpg degeytcsak nqygeaicsv
     2281 yiqpegfgpp pqqqiggyrk eyaqtfqste qksqtgsqsf qqrsyqqtid krsyvngtst
     2341 siedfkvdtf eyrllretef resitrrfvg esdvqistvv drslgpvapp qisqkprnsk
     2401 liegsdavft akisgnpkpr ltwfkngqri resqrvemsh snqqatlrir valpedsghy
     2461 tllsenpqgc tvssaylaie ssdqvdqayq tpresvktpq vestgesdsg kvlppnfvrt
     2521 ctdrdategk mtrfdcrvtg rpypevtwyi ngqqvandlt hkilvnesgn nslmitnvsr
     2581 adagvvtcva rnkagetsfq cnlnviekeq vvapkfverf tttnvkegep vvfmaravgt
     2641 pvpritwqkd gvpitpgpdv ristdgsgas tldipcakls daawyqctaq nvagstatra
     2701 rlfvetpkga apeprrlnlp rptkviepep apgpeviylr hverakpylp ppeedrvypp
     2761 prfiiplrdv hqieggrihf eariepvgdp tmrvewyvng raldassrat sifrfgfisl
     2821 dlisiilqds geyvcrvvss tgvaesratl svtpratieq asqhpdslqy iqqledysky
     2881 qrqesveeis sqrpafirpl qdlgelqegr nahfeaqltp vsdptmkvew ykdgrpitas
     2941 srittifnfg yvslnilhlr aedagsytvr avnrlgeavs saslrvyart svttdlgipe
     3001 qqryieaaee leayqqamhq kyvqeqpept sppefkspik dqnsireggf ahfearlepv
     3061 gdsdlrvewl kdgrpveass rittffnfgy valtikyvti hdvgiytcra ynrageahtt
     3121 aqlsvisknd viydsqhptg lqkiqtleds srysrqvqee tqvtqaprfl gtlkgtnkiv
     3181 egqrahfear vepqsdltmt iewyhngkai taanriqtyh dfgyvaidil qvrpedagty
     3241 tvvarnalge arlsatmvve trssvdtssm hrtsyektqr leeskfvepq yhieeisksk
     3301 pifvqplsdp kpvsegknih lecrlepmgd ptmrvewfqn grpvtvgsrf rtyydfgfva
     3361 ldivhstild sgeytvratn hlgtahtsac vrvigksdiv tetqheqsle qiqmledaar
     3421 yrktqqeevt vmqapqftrp lhnietvelt nvhlecrlqp vgdatmkvew fvngrpvktg
     3481 hrfrpsyefd yvaldilgvy pedsgvytcq arnqlgeavt scsvrvhakk dlllesqhpe
     3541 gleriqyled asrykrhelv devvkvkprf vtapknqenl hegqhahfec klepvtdsnl
     3601 kvewykngrp vtighrfrpi hdfgyvaldv idliaedsgt ytcravnlvg sdevscslsc
     3661 rstaqvltdt kneqgleqih yledrskyqr redveetttq apifttslnn veikegqrah
     3721 fecrlipvsd atmkvewfhn nkpvkagsrf ietnsfgfva ldimyayped sgtytcrakn
     3781 iigeaitsat anvhskksiy tetqneetlq rlhhledtsr yqrkttteei vtqapvftmp
     3841 ikdlkvaenq aahfearvip vgdsklkvew lrngvpiaas nrvttmhdfg yvalnmkyvn
     3901 pedsgtytcr avndlgeavt satlfvqska alqfesqhes alsklqaled tsryqrheee
     3961 eivvkekpsf tvqlngptnl vegqsahyec riepypdpnm kvewfhngkp lstghryrtt
     4021 cdfgfasldv ltvyaedsgt ytckatnklg saqssikldv ksrasiiret qhesalkkiq
     4081 yleddsrykr vdqedliiae kpkfgrplkn iehlpegksa hleatltpvn dptmvvewfr
     4141 dgrpipqghk fkttydfgyv aldilyaype dsgtymckar navgeavttc visvdskqgl
     4201 fldtldaqrl qkireletve vkqevekevv hqkpvfltpl nnldhlkege hahlecrvep
     4261 indpnlkiew fvngvavktg hrfrkthdfg yvaldilyty pedsgtymck atnlageavn
     4321 tctikvgsrr silldtqhpd glekirelea qghparleve eppvtpprfv telrgttevy
     4381 egqtahfecq ieplhdanlr ieffhngkpl psasrfhvtf dfgyvaldig htvpedagey
     4441 svrainalgq cvssielrvi prdniildsq rpegldkire leaqqpwkrp dipepqtrqr
     4501 pvftqplqni daipegqtah fecrlipvgd ptlkvewfrn eipletssri tkvhdfgyvs
     4561 ldinhvreed egvymcrasn plgeavttas mkikskasiq ldtqhpeaqr kiaqleadkp
     4621 srpeepekvf dkpiftqllt gptelwegqm aryecrvvpv gdaslrfewy ingvelkmgs
     4681 rfhvshdfgy vtldilkvit edsgvytcka inkageavss islkvkarsa idadsiqpda
     4741 wqkiqlkeae mnkvpemfvd ttpqqapvft khlesydklv egqhvyleaq vepradpnlr
     4801 vewfkngisl qtgtrlrstf dfglvtlsin glrpddsaiy tckatnllge avstcslkiv
     4861 drhwllgdtl hpdalpkida leqphvtsae qpepiyeepv fithlnnvec vegdnvhfec
     4921 nvepskdptm kiewfingkp lptgarfkst ydfgyvaldi thayeedsgv vivkatnskg
     4981 saqtsgtlkc tskqsiylqt qhpqgeagle kvkeaedayl skyrrpdegp eheypkpiwt
     5041 vplqpefklg efeplhlegq vepkddpnlk iewyfngkvl ehasrfkmts dfgfvtldlt
     5101 dvyerdsgiy tckaynkage aftsttiycs tkeniiersq hpkgkeglea iqdleeslkr
     5161 qegappesee ghppvftsqf enltnlsege iahfeasltp tgdqtmvvew fyngkvleas
     5221 hrirtvyafg mvvlevlgtk iedsgtyscr atnkwgqaei svelqcvdks kgqkpkftth
     5281 iqsleglkdg qsahfectli pvgdpqmkve wfhngqplrh ssrfkmvsdf gfvvmdiagv
     5341 mahdtgeyvc kasnkygedf tkatlkcfgk sgvyldslqp dslarirele sfggeqpttp
     5401 ttpvaeppkf itqivditrl vegqsahfea rltpvndpdl kvewyyngkk lphghryrtf
     5461 hdfgivildi lycyeensgv yecrafnkyg edttkatlkc fskasliles qlpkgmeggl
     5521 ekiqtledsm irtkdekvve ergkapmftv plsnidglre gesahfearl iptddpklkv
     5581 ewfwngkplr tgsrfrtfcd fgfvileisp iypedsgeys cratneygea vttctmkctg
     5641 krsiilesql pkgmestidk iaeleglgne pgqvtpeddt gkppefvttp sdltltensl
     5701 ahfecrltpi ndssmrvewf hngkpllags riktihdfgf vilevancyq rdsglytcka
     5761 tnrhgeatvn cklqvrgrqg iilepqlptn fktgtesiqk leealykrde ilteeetpnp
     5821 pkftvelkdi eveegapshf dcrvepvgds tmridwfhng rsfatgsrvh qindfgfisl
     5881 dmsytyards geyvcratnk wgsattkati tckskktidf dsqlpsgmsg eklkelergp
     5941 vseppveeap rqpprfithi qsatvdesep vrfecrvepk ddpnlriewy rngkmipagh
     6001 ryrtmydmgf vsmdilyvyp edsgeyvcka indlgedttr aslsckklps iilqnqvpkg
     6061 mkksealmqm eatikkytse vhlteddlyd adkkqpprfv tqildqtelv emnsskfecq
     6121 lapvgdpnmk vewffngkpl phknrftpiy dfgyvamnfg wvypedsgey lcratnlygm
     6181 detravirta gkpgiiyesq lpkgmksiek iremeaawqi vpeeegeeek vrapptfvsk
     6241 pepvtveegd csrfccrvtg hprprvmwii nghtvvngsr ykltydgmyh ldipktrqyd
     6301 hgkveviars svgesrtett ltvkprsddy rgvlknsprp wydygltqyq terqntelek
     6361 vfderhhnvs qgieiatehl gqkvfkepet dwqksvkskk neeyynklmt leeeqvlket
     6421 rlresshqfa ipgekvvads vakgmaqkye etleekkdek tqetvqpppr plkkphttev
     6481 didvermkvt gkyppgpses tvhgrevhva kqkqtqkevk gdleitrkit atettevehk
     6541 aktqervvqg ptkpakppvf tkkiqpcraf eqeqarfeve fdgdplptvk wyredfpiqn
     6601 sadlqiytfs tksvlivrqv fmedsgvfsv iaenrggkak csanlvveer rrqpgrggvi
     6661 ppsflstiqn tsvttgqlar fdakvtgtkp ldvywlkngk kitadirykt leedntytll
     6721 lletvpedsg kyecvainka gearcdaect vrgpqspaks akpttpgvek apavleplkd
     6781 qtiregtsva fscrisgkpv ptvqwkkgdk vikpskyfqm skegdfctlr iseafpedeg
     6841 vykciaknpa gdvttsanlr vlapdaadvl pkltplkdqi vsegqpaqfk tqvtpskpkp
     6901 tiqwyregal ipqspdfqmi hegnnavlli attyeedtgt ftcrattsag tvetsakliv
     6961 kkrtgayyev paetgpgart gdkpfvslgt gkdvilepip ldkgeleipl kpgdetlpwr
     7021 kgrvhhrprl adttpwrktk pksrdtsldk lqatesqikp wteetvtlkk tpqqareipk
     7081 ekleevelkp akiekkeirr psletvdlkp ftkettketk ieekltrree idkeiyeeee
     7141 dsrflktsrk vdetvqrkkd evkpwteekv tlkkskpekk elpketletv elkpskigkg
     7201 evrkpslekv dlkpvpttvt ekvsekvtek teyleqedts lldvsrteev kkkrkekvee
     7261 kpekpeqakp wteekitlkk skpvvkeiek etletvelkp skigkaelrk pslekvdlkp
     7321 vpttvtekvt ekitekteyl eqedtslldv srteevkkmr kekveekpeq akpwteekit
     7381 lkkakhlpke ieketvetve lkpskitkse irkssvekvd lktvtketke itqktdyree
     7441 edttllevtk dtqvkktrre sipvekvegp kpwteekvtl kkakpekkev pkekpetvel
     7501 kpsrpqkadi ekpsledvdl kpipkkvtke ktekdkyiee edtsllevtk dtetttkvek
     7561 kvkrreevek kpetkpwtae kitlkkakve qkeirresie evtlkptkte krqvtketle
     7621 kvdlqkvtdk tqfveeedtt lmkvdkvekv eeervdkmat wrrdrkpkek apyheeedks
     7681 ildvkrdees tekrkevpvp wargkkkpte kapyheeedt slldvkkdee siekqkevpv
     7741 pwargkkkpk eekpelvpqq vqpeekpeee etlektqvpw lrdkkvarki perkeepved
     7801 iksvtlkpvr rerapeekek peevtlkpvk ripreeeeke evqlkpipkr kpeekpqeei
     7861 ilkpfqkpkp eveeqekpkl kpfekakpev peqekvqlkp fkrekpevpe kekvelkpfk
     7921 rekpeetlvp qkvpekpeee qpseveevsw rrkpkkkeai ipekpvekve evkvvedkvi
     7981 kkqeetiedf ksftlkptkr gkkleekeek eevtlkpvkr lpkeeetkae iklkpvekkv
     8041 eekeakplkp emaptvpeee kptevsevpw krgkkkpvek rveevtteek kveeeekevs
     8101 elswrkpqke kvkevkkeqe vedftavmlk ptrkekkpee keekeeitlk piqkvqkeee
     8161 kpeavtlkpv kvekpeavek eevpeektel pwrrgkkpak kivpqeevkp eeigkpeerv
     8221 pevkeevpkk pkvekpeeek levklkpvkr lekpeeakpe evtlkpvkrl pkeeeekeev
     8281 klkpikrekp eeekpevklk pfkrlekpee ekpeevtlkp vkrlpkeeee keevklkpid
     8341 rkkpeeekpe vklkpikrle kpeeekpeev alkpvkrlpk eeeekeeikl kpikrekpee
     8401 ekpevklkpf krlekpeeek peeitlkpvk rrpkeeeeke evklkpikke kpeeekpeik
     8461 lkpikrlekp eeekpeevtl kpvkrlpkee eekeevklkp ikkekpeeek pevklkpfkr
     8521 lekpeeekpe eatlkpvkrl pkeeeekeev klkpikkekp eeekpevklk pvrrlekpee
     8581 ekpeevtlkp vkrlpkeeed keevklkpik kekpeeekpe vklkpvkrlp keeeekeevk
     8641 lkpikkekpe eekpevklkp vkrlekpeee kpeevtlkpv krlpkeeeek eevklkpirk
     8701 ekpeeekpee iklkpvkrld kpeeakpeev tlkpvkrlpk eeeereevkl kpvkkekpee
     8761 ekpeeiklkp vkrlekpeet kpeevslkpv pkvvpekdee kpeeeapets ilpwrrgkkt
     8821 kkvvdfheev tvvpidqkki vsevhekett iveqkveake viekkvvvee vkpevveevt
     8881 evsevswrkp rkpkkpveek evvelkpvek heeivekvee kitlkkvkke vpeveekkei
     8941 qlkpvpkkpk eeevpekitl kpvkkekpee kpkeeeipel kpvklekpek veeteekeev
     9001 mveitrkkpk kkpeevpvek eevveteite iteekpeeis vlpwrrpkkv tekkieveei
     9061 kpekvedkev eevtettevs wrkqrkpkkp ieeeekpekv tlkpvkrekp eekpkeeipe
     9121 lkpiklekpe kveeteekee vkvevtrrrp kkkpeeiqre eievetkevv ddvevekiek
     9181 qeiqiedrkr krkqrtqvdi siidkdkkia prfiqklqpv iaepestakf tctvfgspvp
     9241 eiswyrneqe lhasekyimt vyettaslei tkvkeedagm yscrasnpag vatstvnlvi
     9301 fekeeegiap hfstpikplm veehkpalle cvvtgspvpe vkwyrgeqel kpkkgreitf
     9361 npetgeaklh ilepteedet iyrvravnkf graecranll innvvkvskp evlrapkitr
     9421 plpalvaerg kpltlsadfe skptpevkwf rngvelapse knviniyent aelfipevtk
     9481 kdggkyeirv qnpvgearss gsvtvkdrpd ktdevkaprf ieplqpqiva egevvimetk
     9541 vesyptasfq wfhetrples tpqvrivtqe nksilmikei kseyagtytc raenvggsvt
     9601 ctatinllet pweetvelvs ptfvkrlspv rvmdgesanl tcvvqgkptp kvewyhdskp
     9661 ikegkeitiv qdtegvcsla itevfpedag eytcravnpv geavcttsli veayeyvpds
     9721 eiassivats lttgqsgsee dllspketpl fdtdteesap eiikklpqli ptkdgeltrl
     9781 evkvkgkpkp egkwykqgve ivssqefqie efedgtsvlt iaetypddtg eivfeahnpl
     9841 gvsttmtyls vegivgtkey rkpewvthme emqealratq tvprfiqeit dvyvkegdta
     9901 vfecsysgnp tpdvvwyknd kmlmstqnlk vriydddkkt vltikhatee ddgayvckit
     9961 setglatska klhvtdvtde kkfyeeeeev eeqtievkpk ekpqkkklrk akevkekvkt
    10021 ervkvdkkev rkeklvpkeq peekkivdie etqilestdi ieekptevsq akkivpiqep
    10081 vlteataslk kiddekpkdm vpkpeerakr vieeregivv vsevttedma pdftveskvd
    10141 raqitseslq tvtvsevhte tsvqevrrae akqrkakktv vteeeifeer pkevvveekp
    10201 kkkkktekvk vreevtieei verqreniar eveeimemlh akefgpgeap lrelatigyl
    10261 vrqgvsvnei neslyrtenf palktpeaqn alvqlvereg hgplitqvlt eetttdesvv
    10321 aatvgfrafm rmielqhvtv eevlthfape afrprawevt eateveteer vtervdvvrk
    10381 tevhfmerrd erkavrtqdi rdikeyedtr qthttlrark drkpreetke drdegvqvve
    10441 ieeiedteqe kmkkkrkdve eieeeietvi vetdskgrla qkkkkrddle ekedveeeee
    10501 iiekvrkelk pkrpkigrde veleeieefd vtedksmpsi mvnvpsqrhq vvpldrtteq
    10561 apgkpeleka kltvdtital tehivpvqek eisdisrikp ierkaslsis pvepysttet
    10621 tvqastgefa dtfkptsyea tpgiipsegv qvsetlvsda gvtslsvskq eigrtaevsv
    10681 tlqeattvye tmvrekevpt edfvtpqtvk aedtilpqls lsvyevhegl tedkleplkt
    10741 vatkprvnvt aveplvveev raedkpgkyy pelivpteva tqsvisqrqr vteemhapek
    10801 egeyipgrlp psqvaqigis ygnetavvqq nvvqesegvf isekktetfe attnvtvleg
    10861 vsvstvqtqd tetsltieet kkavadlnvi emtpavtvet vpsekekeyq pgdkpdtkta
    10921 etsissleig siqstvvqes egvyrpdekp tkalaetsir peehvmisei qtadypsdfr
    10981 eelkyvqesg tvsvqlteak miqetlthdr eermeemvkp eermvdtsyd vnrsvevfqt
    11041 tsvekeadlr vfempeshrg ktvpthpvvs levemtqped nlgevkkevp ssgtakvehv
    11101 slqetivget vvaenlapve kdkapetkva eiamnevesl httmvvvsek eteytqvsdv
    11161 kgayasteft tqvapmleev rshspteeyl pdqkptsgia qpshvplesi tvavqelaek
    11221 edlyktdvrp dqktanvalt eltsisipev mqvitassae tlkketkmke ekgrveveel
    11281 sstiisevis netedvfttk atpkdqkadf nisgreaaet tqvtamteae dlavkrpeeq
    11341 kgkpqvdelt pltvshvetg eaekelptae vpkektaqpn ifgrdvaetm qvltvtsaee
    11401 laapkapegq kgkpqveelt plsvsqvvsq eaesalpape apterkaqpd mtgrdiaetm
    11461 evltmtsadi laedvkpteq kgmpgleefv plsvsqtvst ekedvlpspe apttktaqps
    11521 ltgrdiaetm qvvmmtseke litalapeth kgipnveelt pltvsqtvsh eaedvfekta
    11581 apsevtakpa fsgrevaqts emmivtnvge lekqvlpdeq kgqaqldelm pltvsqvisg
    11641 eveeglpeae kpkektaqpg ilgrdiaett qvltvtsaee laapkapegq kgkpqveela
    11701 pltvsqvvse eaegilpape apterkaqpd mtgrdiaqtm evltmtsvdt ftedkkpdeq
    11761 kghpeleefv plsvtqtvsn emedvlpspe apstkiaqps ltgrdvaetm qiltmtseee
    11821 litaqtpeqh kgkpnveelt pltvsqtvsq eaeeilekaa aptevtakpa fsgrevaqts
    11881 eimivtnvge lekqvlpdeq kgqpqldelt pltisqiisg eveeglpeae kpkektaqpg
    11941 ilgrdiaett qvltvtsaee leapkapegq kgkpqveela pltvsqvvsq eaegilpvpe
    12001 apterkaqad ltgrdvaemm evltmasvdt ltedkkpaeq kgvpgleefv plsvsqtvst
    12061 ekeevlpspe apttkvaeas ltgrdiaetm qvvtmtseee lisarvpeqh kgifnveelt
    12121 sltvsqtish eteeilekaa algevtakpa ftgrevaqts etlivtnvge lekqilpeeq
    12181 kgkprvdemt sitvsqvvsg eveerlpeae kpkektaqps mlgrdiaett qvltmtsaee
    12241 laapkapeeq kgkpqveela pltvsqvvsq eaegvlpvpe apterqaqpd ltgrdvaqit
    12301 vvmsmasadv middkkpeeq kgvpgveefv plsvsqtvst ekedvlpspe apttkmaqps
    12361 ltgrdiaetm qvvtmtseee litalapeth kgkpnveelt sltvsqtisq etevileksa
    12421 apseatakpa ftgreiaqts eilavlnvge lekhvlpeeq kgkpkleelt pltvsqiisg
    12481 eaeeglptae vpkektaeps mlgrdiaett qvltmasaee lmapkapeeq kgkpqveela
    12541 pltisqvvsq eaegvlpvpe apterkaqpd ltgrdvaqtt vvmsmanvdi mieekkpeeq
    12601 rgipgleefv plsvsqtvst ekedvlpspe vpttkmaqps ltghdvaetl qvltmvseeq
    12661 lttkapeeqk gkpnveelts ltvsqtvshe aedilekava pseatakpaf lgrevaetsq
    12721 vltvttvgel qkdvlpeeqk gkpqleelmp ltvsqivsge aeerlpeaek pkektaepsm
    12781 lgrdiaettq vltmasaeel lapkvpeeqk gkpqveelap ltvsqvvsqe aegtlpipea
    12841 ptertaqpdl tgrdvaetme vltmasldtl iedkkpaaqt gapgleefip lsvsqmvste
    12901 kedvlpspev ptekmaqpsl igrdvaetmq vltmisamel ttghapdqqk gvpnveelts
    12961 ltvsqtvshe teeilekava pstvtakaal sgrevaetsq vltvsnveef eksilpegqk
    13021 gkprldelts itvsqvlsne leetlpspek psekiaqpqm igrevaeqte vltvtnveel
    13081 kklekpegqt gkpdveemsf itvsevfste mekempspev pkertalpql ssievaette
    13141 vltmisaeel lkskapeeqk vqpkldeltp ltvshvvsee aeealptaei pkektaqpsl
    13201 lgrdvaetsq vltmasaeel laakvpeeqk gkpqieelap ltvsqvvsqe aeealpsaev
    13261 pvereaqpsm tsrdvaqtml vtsmsnvdim aeskipeeqk gvpgleefvs lsvsqtvste
    13321 kedvlpspev ptekmaqpsl igrdvaettq iltmisatel ttdrapeqqk gvpnveelts
    13381 ltvsqavshe teeileklea pgtvtakaal sgrevaetsq vltvsnveef eksvlpeeqk
    13441 gkprldelts itvsqvvsne leetlpspek psekvaqpqm igrevaekte vltvsnveem
    13501 qklekpegqk gipdveemsf itvsevvste aekelpapea pkerkalpql sgievaekse
    13561 vltmtmteef akpkapeeqk gkpqleells lsvsevalge vekglptpdk pteetakpsl
    13621 lgiqvaeksq viassitgel qesikpdtkq iipeqipfes ieqsqtltqe restfvvekt
    13681 ttsataevsf rvseslevtq vtatekeske vikgvakeas aqadvierkv alktevqped
    13741 masefkvkkp egktakgvde iqqgvivteh ltahefesel pesvlpfakl arvsiqadhl
    13801 ehivetteap aqqhhititq httkhdlpqq qsiesdtevi eeyttkfrrg stedataavk
    13861 tkktivrkkp kttgeedivv vieeelddik pkipsipkkq fmpieevttv sdveeivsar
    13921 ideaeeivpk eeekvevtit evqdtdkkkk vtkkqfvrrr gktrqvteei tveeegkape
    13981 ttvvtkpvei ieedieerpk iekeeyvvee vpeektiplk prkkltptvq qveeqvtvte
    14041 eitkegkprk ttkkkvitrk gkeqqvtevv tveeqgkapi tsvtegpvee vveeiiqklp
    14101 apkyakpsev eevreqvtvt eevtrlgkpk kttkkkiikr kgkeqqvtev vtveeegkap
    14161 tttvtegpve evvieeikel pipefvkpte veevreqvtv teeltkmgkp kktikkkiik
    14221 rkgkeqqvte vvtveeqgka pvttvkegpv eevveetitp llapeyakps eveevreqvt
    14281 vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvtegp veeiieeeik
    14341 pefikpreve evreqvtvtq evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv
    14401 ttvtegpvee vvdetvkalp apeyakpsev eevreqvtvt qevtklgkpk kttkkkiikr
    14461 kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe fikpreveev reqvtvtqev
    14521 tregkpkkti kkkiikkkgk eeqvtevvtv eeqgkapvtt vnegpveevv detvkalpap
    14581 eyaepsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    14641 tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr egkpkktikk kiikkkgkee
    14701 qvtevvtvee qgkapvttvn egpveevvde tvkalpapey aepseveevr eqvtvtqevt
    14761 klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv tegpveeiie eeikpefikp
    14821 reveevreqv tvtqevtreg kpkktikkki ikkkgkeqqv telvtveeqg kapvttvteg
    14881 pveevvdetv kalpapeyak pseveevreq vtvtqevtkl gkpkkttkkk iikrkgkeqq
    14941 vteivtveee gkapkttvte gpveeiieee ikpefikpre veevreqvtv tqevtregkp
    15001 kktikkkiik kkgkeqqvte vvtveeqgka pvttvtegpv eevvdetvka lpapeyakps
    15061 eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt eivtveeegk apkttvmegp
    15121 veevvieeiq elpipefvkp teveevreqv tvteevtrlg kpkkttkkki ikrkgkeqqv
    15181 teivtveeqg kapvttvteg pveevvdeti kslpapeyak pseveevreq vtvtqevtkl
    15241 gkpkkttkkk ivkrkgkeqq vteivtveee gkapkttvte gpveevveee vkpllapefv
    15301 kpseveevhe qvtvtqevtr egkpmktikk kiikkkgkaq qvtevvtvee egkvpvttvk
    15361 egpveevvde tvkalpapky akpseveevr eqvtvtqeit klgkpkkttk rkvikrkgke
    15421 qqvtevvtve eegkapettv tegpveevle eeikplpgpe fvkpseveev reqvtvteev
    15481 tregkpkkti kkkiikkkgk eqqvteivtv eeqgkapvts vtegpveevv detikqlpap
    15541 kyakpsevee vreqvtvtqe mtklgkpkkt tkkkiikrkg keqqvteivt veeegkapkt
    15601 tmtegpveev veeeikplpa lefvkpseve evreqvtvte evtregkpkk tikkkiikkk
    15661 gkeqqitevv tveeqgkapv ttvtkapvee ivdetikelp alefakpsev eevreqvtvt
    15721 kevtkegkpk ktikrkvikk kgkeqqvtei vtveeegkap kttitegpve evveeeikpl
    15781 palefvkpse veevreqvtv teevtregkp kktikkkiik kkgkeqqvte vvtveeqgka
    15841 pvttvqegpv eevidetiks lpapeyakps eveeireqvt vteeltklgk pkkttkkkit
    15901 krkgpeqqit eivtveeegk apvttvtegp veevvkevlk plpvpkvvkp seveeireqv
    15961 tvteemtkeg kpkkiikkkv ikrkgreqtv qevvtveekg kapvttvtvg pteeiveetv
    16021 ktlpapeyak pseveevreq vtvtqevtke gkpkkttkkk vikrkgkeqq vtevvtveeq
    16081 gkapvttvte gpveevveel lkpliapeyv kpseveevre qvtvteeltk egkpkkvikk
    16141 kvikrrgreq qvkevvtvee qgkapvttvt egpteeivee tikalpaped akpseveevr
    16201 eqvtvtqevt kegkpkktik kkvtkrkgke qqvtevvtve ekgkapvttv tegpveeive
    16261 etvkalpape lvkptevtev peeltvtevv tkegktkkiv kkkvpkpkke gieapeeape
    16321 evpveetkki iklqrlkvsk festkldiqe tslgkpqfaq iklrktpvak rpskkdekfp
    16381 rillrsriia iewppspknl kvdtfepiel qngilsrnvd eaaalpkpkk kkikriekei
    16441 aklekldqef eelkkelple kleepileek perkvkkkkk pedkakegvk pqlmrisike
    16501 qkpikpkiae patplfaqik lkkapakpkk kpeeevskfp killrsrita hewppsvkyp
    16561 aisvlepnyi qngilsrtme ealklkkvkh kkmklpekel telekldqef eelkkvplek
    16621 veeitpyerk pkpekpeeek pkklvigkgk rrpaeeedle tvqlkkipek ipsapeeaek
    16681 vpkkkdevdk pkkekeekey pelkpfepyd ieapevelee rekpeypepe kpkekkvpgk
    16741 kpkkerktpa qeteavpivp gvpkpkepee eeeikrripe rpppteepek iklkpwkkge
    16801 kpeeeekkpe dyikfvpkdd divetvevtt tveetpdkkq rkikkkktit kpkdekpkii
    16861 eettieeegf epvvtveefd vdkyeavpve svekaapvee ikeeivtsei iteegtkktv
    16921 rkkrvtkkre gkervteevt veeegkepit atielhedti seiheqakhk rptkpkadek
    16981 laedvtektv eqqeiieevk evkptkkvik kekpvkakde vteevveeii ieepekqeak
    17041 piveeeetvi itehdvrkap kkkkevvike eeeifetiet pthkiirkki rpmkkgekev
    17101 vveeiiqkpr eetvemeeqq ivevvetptk kivkkkkevv kddgvpeelv eeiiiekpqk
    17161 kkakptveee kkkvkvtkvd tkkapekpee vveeeeeiie tietptkkii rkkpkdkkdk
    17221 iieeviqkti eeakameeqe ivevietptk kvikkkttti eegkapeekv eeiviekpke
    17281 vieeeeeiie tietptkriv rkkpkdkkdk iveeviqkti eeaktmkeqe iievietptk
    17341 tvikkkkati kegkapeeev eeiviekpik ekakkkilpk egveitevit etsveeliee
    17401 elkpeeakps eeveeekvki tevaikkvpk kkkpskavea eekeevveei kpekpkeeka
    17461 kptvtekegi evteviseva peklpeeakp kkkkateike teekvitkkv kvkeapkeav
    17521 pgeeeqpaee vdelvpekpk ktkakkevtp qdsivttevv pitkeeelev ekpeeekatv
    17581 veekeekakv eekkvtvkka pkkklkptea eeeqveevve eitpeepkkr kakkavipke
    17641 eiqttevipg itaeeipeee kpeeekavpk eeekveeeak vtevsvkkke rkkkekpvtn
    17701 gevieekpkv keipeekpke evpqvpeeke kpeeekpkev kpkekkpkee kpkkpieekp
    17761 eevkpeeekp evkpeeekpk eekpkkkkpa ekkpkiseee rpedkveeip ekkpeevktr
    17821 eeiipkdtvq ttevipetva eeiptkkpke rkavpveeke ekveitevpv ekapekkeek
    17881 vpeekpteaa pekpkeakvm kkkkkkpakk deieewvepe yerpvlepmp ekivwepkkk
    17941 kekkplpesl qklvpqkier kemkptklky sepaetvqfa siklkkvavp kkkeadetkf
    18001 pkimlrsrmt fvgdyppelq ipkityleen pvqtgilsrn teealkvlkr kvkkvklsek
    18061 evtqlekldl efeelkkvpl ekiedksvye rqpkkpaekp eepkkvpigk gkvpeeekve
    18121 pekvklkkvp ekkpeeveep lkkpkkpepe eevkpekkkk pkvklehdel kpldfdrpel
    18181 ekyepeeyek peepapepvp kpyekepkkk pdqeveqipl kkgkpkspep deepevkfri
    18241 ptkekpeeep eqitlkpwkk ekpeeekeke ipkkeeekvp kppkeepedv tvqrkaktkk
    18301 pkekpkeeke eevtvkkpee ekpeevaeev tlkkepekpv eeapaevtik kpvveekeve
    18361 eeetitevil kkkpekpeev veeavvkkpk kkkpvkeeev veevtvtkvv teeekeeekv
    18421 vikkpkkksi peiteevtee itlkkpeeka peevteeikk pkkkkpvkee vaeeitikkp
    18481 vveekkeeev peevtlkpkk peekpvieev teeitlkkpv peevteevai kkpkkkkpve
    18541 eeaadeitlk kpvvvekeee eiteevtlqk kkkpekkpvp eeiseeitlk kpvpkeeeka
    18601 peevteeiai kkpkkkkpvk eeaadevtik kpvveekkee etpeevtlkp rkpkekpvpe
    18661 evkeevtlkk pvpeekekvp eevteeiaik kpkkkkpvee eaadevtikk pvvvekeeee
    18721 iteeitlgkk kkpekkpvpe eiteeitlkk pvpkeeekap eevtekievk kpkkkkpvee
    18781 eaadevtikk pvvvekeeee apaeitlkrr pkekpveeit eevteeitik kptpeekeka
    18841 peevsekvev kkpkkkkpvv eeaadevtik kvvveekkee eapeevtlkp rpkkksvtee
    18901 evaevtltkp kpieeekkpe eseltikkkp kkkpvieeea aevtikkpvp vepdeapeei
    18961 tirkkkkpek kpkvteevee tavtikkara pekpteeeei ttdvqikkkk pqkkveevaa
    19021 deltikkiee veqpeeeeev qeftfkrkpp klppkpieei yedvtlrklr pkrksrpdik
    19081 eitetenvtf rprttktked veqefkisln tyeeedisms gkvrlkpkkk pltyseetge
    19141 etiriiqeve ddsgpiieei iesdeegkde ysieeldtde mrlplrrkkk kvpkpykved
    19201 ieegdvklkl krerkysiee adeslalklk skrrvstyde eeatlsitre ediseeveie
    19261 yvvrdgdtmf sicsyvaetd eainlveger vyvidhtnqd wwfvkkhlte ekgwvpaqyl
    19321 lnevhytlyl qrklhekidk lpvfekpapg dkssapkfve klqpihtpdg ytiqfecqve
    19381 gvprpqitwf rqtaiikpsp dfqmyydddn vatliirevf pedagtftcv aknaagfass
    19441 tteliveapl sdhgsdltgp srkslsress ladilegipp tfsrkpkaky vdegedvile
    19501 crlvavpepe itwyykdtqi ttkeniiiat esdmhmycsv ikitkvqkkq egkytivarn
    19561 regeatieip mkvktgksep peileplqsy vvregetvvl stqivgnpap kvtwfkngkp
    19621 lkgltpkqdg hvntltliqp qvsdtgeysv vatndlgtae tkatltvein lkpslainfq
    19681 qhtfehfsvt keipsgapep plfterfqel tvpekgtfkl vakvtgnpvp evtwlrnnkp
    19741 lekspnitet ydgenivlei rnansevdtg dykcvasnpv gkashgarvt vdvdkvtftk
    19801 tlekevvvde yktleltcet shtvstkwwh ndkeisgmdh reiieegrvh klvikktnpt
    19861 degtykctvk nqstssnvtv katkpefvkk lqdcevkerd vaileveits qtvdvkwykd
    19921 geqlgpgkek lefvkegtvr kllirstsvh degeytcsll dqectaevtv ielppeiitk
    19981 mqdvtiarge ratfdieltk gdalvrwfkd gqelqfsehv rlsidgkrqk lkiydtesed
    20041 agvyscevgq qkssakltve epavefitkl pdvtlvplns davfvielsq dvpvtwmrkt
    20101 qvikespkyt iidegkvkkl ivkkcttedi seytamvanv ktssrlrvev ietppkispd
    20161 tprkykvrkd edvdivvkfs gtpkpedewt vngrvvtksk raiptidees avltirkiqe
    20221 edvgdytlkl vnsvgetsie invvivqvps apgapeplet tndsvtlhwk kpdsdgnspi
    20281 veyilehqek tettwtkite titetthkvt klttnkeytf rvtavneagp getsptspyv
    20341 kiskpstaep pvileplksi viglgetvtl scviggtpap eitwlrndei fedtsityen
    20401 hitkytiret tetssakftv kaknnvgsae ttcelkiqes pkiiydenlt nqklpmngqw
    20461 kieiqitgfp kpevtwtkng kevdekrvsi hreertstvs isslvredta tymakaenqa
    20521 gsssvdlhlk vidkpskpqg pvvfkeirqd rvtlewrppa ddggieleky tiekcepgkt
    20581 wvkvvdidke vesfcvhklq qnaeykfrii arnavgasep lesdtvkmrt sfeppgaprg
    20641 plevsgmtkt sftikwqppe ndggtpitdy iieikeeskk twqkigstkh etthfsvsdl
    20701 ktdvpynfri taknsvgsgp pyvaeepiap grrikitklt essmyallgi fpptkivisk
    20761 tppsspqhvh vtnitsksat lswsppastg gteltgyvie krpligkgar wtkvvtldat
    20821 tlqycvenlk eseflfrifa ennqglsvpa nsepvtlkth asvpspptap leirqiaant
    20881 vviswgrpds dggaplegyk vairdtkrtm wmevarvnte iqklnirdlq enhmylirif
    20941 arneiglsdp leseepfkii paseltvvep iaeatekget asisfstent sswlrehnmd
    21001 adihsyarar llrqdeyffr iwhyakklfe
//
LOCUS       XP_034182902           20889 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X15 [Osmia lignaria lignaria].
ACCESSION   XP_034182902
VERSION     XP_034182902.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327011.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182902.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20889
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..20889
                     /product="sallimus isoform X15"
                     /calculated_mol_wt=2359730
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1318..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4272
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16500..>16652
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          19127..19179
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(19132,19134,19140..19141,19158..19160,19173,19175,
                     19177..19178)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          19215..19305
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19232..19236
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19245..19249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19271..19275
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19285..19290
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19298..19301
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19339..19432
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19356..19360
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19369..19373
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19394..19402
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19412..19417
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19425..19428
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19440..19526
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19457..19461
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19470..19474
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19492..19496
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19506..19511
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19519..19522
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19560..19650
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19577..19581
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19590..19594
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19615..19619
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19630..19635
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19643..19646
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19663..19740
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          19673..19677
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19684..19688
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19709..19713
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19723..19728
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19744..19829
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19761..19765
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19773..19777
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19799..19803
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19813..19818
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19822..19825
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19834..19918
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19851..19855
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19863..19867
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19888..19892
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19902..19907
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19911..19914
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20024..20104
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20032..20036
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20045..20049
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20070..20074
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20084..20089
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20097..20100
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20108..20199
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20108,20173,20188)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(20189..20190,20192..20193)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          20210..20296
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20227..20231
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20240..20244
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20262..20266
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20276..20281
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20289..20292
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20320..20390
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20320..20323
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20332..20336
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20356..20360
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20370..20375
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20383..20386
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20394..20486
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20394,20460,20475)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <20464..20779
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(20476..20477,20479..20480)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..20889
                     /gene="sls"
                     /coded_by="XM_034327011.2:749..63418"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    13501 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    13561 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    13621 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    13681 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    13741 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    13801 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    13861 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    13921 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    13981 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    14041 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    14101 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    14161 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    14221 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    14281 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    14341 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    14401 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    14461 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    14521 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    14581 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    14641 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    14701 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    14761 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    14821 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    14881 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    14941 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    15001 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    15061 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    15121 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    15181 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    15241 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    15301 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    15361 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    15421 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    15481 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    15541 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    15601 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    15661 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    15721 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    15781 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    15841 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    15901 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    15961 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    16021 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    16081 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    16141 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    16201 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    16261 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    16321 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    16381 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    16441 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    16501 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    16561 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    16621 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    16681 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    16741 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    16801 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivdvtektve qqeiieevke
    16861 vkptkkvikk ekpvkakdev teevveeiii eepekqeakp iveeeetvii tehdvrkapk
    16921 kkkevvikee eeifetietp thkiirkkir pmkkgekevv veeiiqkpre etvemeeqqi
    16981 vevvetptkk ivkkkkevvk ddgvpeelve eiiiekpqkk kakptveeek kkvkvtkvdt
    17041 kkapekpeev veeeeeiiet ietptkkiir kkpkdkkdki ieeviqktie eakameeqei
    17101 vevietptkk vikkktttie egkapeekve eiviekpkev ieeeeeiiet ietptkrivr
    17161 kkpkdkkdki veeviqktie eaktmkeqei ievietptkt vikkkkatik egkapeeeve
    17221 eiviekpike kakkkilpke gveitevite tsveelieee lkpeeakpse eveeekvkit
    17281 evaikkvpkk kkpskaveae ekeevveeik pekpkeekak ptvtekegie vtevisevap
    17341 eklpeeakpk kkkateiket eekvitkkvk vkeapkeavp geeeqpaeev delvpekpkk
    17401 tkakkevtpq dsivttevvp itkeeeleve kpeeekatvv eekeekakve ekkvtvkkap
    17461 kkklkpteae eeqveevvee itpeepkkrk akkavipkee iqttevipgi taeeipeeek
    17521 peeekavpke eekveeeakv tevsvkkker kkkekpvtng evieekpkvk eipeekpkee
    17581 vpqvpeekek peeekpkevk pkekkpkeek pkkpieekpe evkpeeekpe vkpeeekpke
    17641 ekpkkkkpae kkpkiseeer pedkveeipe kkpeevktre eiipkdtvqt tevipetvae
    17701 eiptkkpker kavpveekee kveitevpve kapekkeekv peekpteaap ekpkeakvmk
    17761 kkkkkpakkd eieewvepey erpvlepmpe kivwepkkkk ekkplpeslq klvpqkierk
    17821 emkptklkys epaetvqfas iklkkvavpk kkeadetkfp kimlrsrmtf vgdyppelqi
    17881 pkityleenp vqtgilsrnt eealkvlkrk vkkvklseke vtqlekldle feelkkvple
    17941 kiedksvyer qpkkpaekpe epkkvpigkg kvpeeekvep ekvklkkvpe kkpeeveepl
    18001 kkpkkpepee evkpekkkkp kvklehdelk pldfdrpele kyepeeyekp eepapepvpk
    18061 pyekepkkkp dqeveqiplk kgkpkspepd eepevkfrip tkekpeeepe qitlkpwkke
    18121 kpeeekekei pkkeeekvpk ppkeepedvt vqrkaktkkp kekpkeekee evtvkkpeee
    18181 kpeevaeevt lkkepekpve eapaevtikk pvveekevee eetitevilk kkpekpeevv
    18241 eeavvkkpkk kkpvkeeevv eevtvtkvvt eeekeeekvv ikkpkkksip eiteevteei
    18301 tlkkpeekap eevteeikkp kkkkpvkeev aeeitikkpv veekkeeevp eevtlkpkkp
    18361 eekpvieevt eeitlkkpvp eevteevaik kpkkkkpvee eaadeitlkk pvvvekeeee
    18421 iteevtlqkk kkpekkpvpe eiseeitlkk pvpkeeekap eevteeiaik kpkkkkpvke
    18481 eaadevtikk pvveekkeee tpeevtlkpr kpkekpvpee vkeevtlkkp vpeekekvpe
    18541 evteeiaikk pkkkkpveee aadevtikkp vvvekeeeei teeitlgkkk kpekkpvpee
    18601 iteeitlkkp vpkeeekape evtekievkk pkkkkpveee aadevtikkp vvvekeeeea
    18661 paeitlkrrp kekpveeite evteeitikk ptpeekekap eevsekvevk kpkkkkpvve
    18721 eaadevtikk vvveekkeee apeevtlkpr pkkksvteee vaevtltkpk pieeekkpee
    18781 seltikkkpk kkpvieeeaa evtikkpvpv epdeapeeit irkkkkpekk pkvteeveet
    18841 avtikkarap ekpteeeeit tdvqikkkkp qkkveevaad eltikkieev eqpeeeeevq
    18901 eftfkrkppk lppkpieeiy edvtlrklrp krksrpdike itetenvtfr prttktkedv
    18961 eqefkislnt yeeedismsg kvrlkpkkkp ltyseetgee tiriiqeved dsgpiieeii
    19021 esdeegkdey sieeldtdem rlplrrkkkk vpkpykvedi eegdvklklk rerkysieea
    19081 deslalklks krrvstydee eatlsitree diseeveiey vvrdgdtmfs icsyvaetde
    19141 ainlvegerv yvidhtnqdw wfvkkhltee kgwvpaqyll nevhytlylq rklhekidkl
    19201 pvfekpapgd kssapkfvek lqpihtpdgy tiqfecqveg vprpqitwfr qtaiikpspd
    19261 fqmyydddnv atliirevfp edagtftcva knaagfasst teliveapls dhgsdltgps
    19321 rkslsressl adilegippt fsrkpkakyv degedvilec rlvavpepei twyykdtqit
    19381 tkeniiiate sdmhmycsvi kitkvqkkqe gkytivarnr egeatieipm kvktgksepp
    19441 eileplqsyv vregetvvls tqivgnpapk vtwfkngkpl kgltpkqdgh vntltliqpq
    19501 vsdtgeysvv atndlgtaet katltveinl kpslainfqq htfehfsvtk eipsgapepp
    19561 lfterfqelt vpekgtfklv akvtgnpvpe vtwlrnnkpl ekspnitety dgenivleir
    19621 nansevdtgd ykcvasnpvg kashgarvtv dvdkvtftkt lekevvvdey ktleltcets
    19681 htvstkwwhn dkeisgmdhr eiieegrvhk lvikktnptd egtykctvkn qstssnvtvk
    19741 atkpefvkkl qdcevkerdv aileveitsq tvdvkwykdg eqlgpgkekl efvkegtvrk
    19801 llirstsvhd egeytcslld qectaevtvi elppeiitkm qdvtiarger atfdieltkg
    19861 dalvrwfkdg qelqfsehvr lsidgkrqkl kiydteseda gvyscevgqq kssakltvee
    19921 pavefitklp dvtlvplnsd avfvielsqd vpvtwmrktq vikespkyti idegkvkkli
    19981 vkkcttedis eytamvanvk tssrlrvevi etppkispdt prkykvrkde dvdivvkfsg
    20041 tpkpedewtv ngrvvtkskr aiptideesa vltirkiqee dvgdytlklv nsvgetsiei
    20101 nvvivqvpsa pgapeplett ndsvtlhwkk pdsdgnspiv eyilehqekt ettwtkitet
    20161 itetthkvtk lttnkeytfr vtavneagpg etsptspyvk iskpstaepp vileplksiv
    20221 iglgetvtls cviggtpape itwlrndeif edtsityenh itkytirett etssakftvk
    20281 aknnvgsaet tcelkiqesp kiiydenltn qklpmngqwk ieiqitgfpk pevtwtkngk
    20341 evdekrvsih reertstvsi sslvredtat ymakaenqag sssvdlhlkv idkpskpqgp
    20401 vvfkeirqdr vtlewrppad dggielekyt iekcepgktw vkvvdidkev esfcvhklqq
    20461 naeykfriia rnavgasepl esdtvkmrts feppgaprgp levsgmtkts ftikwqppen
    20521 dggtpitdyi ieikeeskkt wqkigstkhe tthfsvsdlk tdvpynfrit aknsvgsgpp
    20581 yvaeepiapg rrikitklte ssmyallgif pptkiviskt ppsspqhvhv tnitsksatl
    20641 swsppastgg teltgyviek rpligkgarw tkvvtldatt lqycvenlke seflfrifae
    20701 nnqglsvpan sepvtlktha svpspptapl eirqiaantv viswgrpdsd ggaplegykv
    20761 airdtkrtmw mevarvntei qklnirdlqe nhmylirifa rneiglsdpl eseepfkiip
    20821 aseltvvepi aeatekgeta sisfstents swlrehnmda dihsyararl lrqdeyffri
    20881 whyakklfe
//
LOCUS       XP_034182913           20888 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X16 [Osmia lignaria lignaria].
ACCESSION   XP_034182913
VERSION     XP_034182913.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327022.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182913.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20888
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..20888
                     /product="sallimus isoform X16"
                     /calculated_mol_wt=2359602
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1318..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4272
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <16500..>16652
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          19126..19178
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(19131,19133,19139..19140,19157..19159,19172,19174,
                     19176..19177)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          19214..19304
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19231..19235
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19244..19248
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19270..19274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19284..19289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19297..19300
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19338..19431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19355..19359
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19368..19372
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19393..19401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19411..19416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19424..19427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19439..19525
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19456..19460
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19469..19473
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19491..19495
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19505..19510
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19518..19521
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19559..19649
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19576..19580
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19589..19593
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19614..19618
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19629..19634
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19642..19645
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19662..19739
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          19672..19676
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19683..19687
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19708..19712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19722..19727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19743..19828
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19760..19764
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19772..19776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19798..19802
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19812..19817
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19821..19824
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19833..19917
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19850..19854
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19862..19866
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19887..19891
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19901..19906
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19910..19913
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20023..20103
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20031..20035
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20044..20048
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20069..20073
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20083..20088
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20096..20099
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20107..20198
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20107,20172,20187)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(20188..20189,20191..20192)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          20209..20295
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20226..20230
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20239..20243
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20261..20265
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20275..20280
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20288..20291
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20319..20389
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20319..20322
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20331..20335
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20355..20359
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20369..20374
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20382..20385
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20393..20485
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20393,20459,20474)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <20463..20778
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(20475..20476,20478..20479)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..20888
                     /gene="sls"
                     /coded_by="XM_034327022.2:749..63415"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikeyedtrqt httlrarkdr kpreetkedr degvqvveie eiedteqekm kkkrkdveei
    13501 eeeietvive tdskgrlaqk kkkrddleek edveeeeeii ekvrkelkpk rpkigrdeve
    13561 leeieefdvt edksmpsimv nvpsqrhqvv pldrtteqap gkpelekakl tvdtitalte
    13621 hivpvqekei sdisrikpie rkaslsispv epysttettv qastgefadt fkptsyeatp
    13681 giipsegvqv setlvsdagv tslsvskqei grtaevsvtl qeattvyetm vrekevpted
    13741 fvtpqtvkae dtilpqlsls vyevheglte dkleplktva tkprvnvtav eplvveevra
    13801 edkpgkyype livptevatq svisqrqrvt eemhapekeg eyipgrlpps qvaqigisyg
    13861 netavvqqnv vqesegvfis ekktetfeat tnvtvlegvs vstvqtqdte tsltieetkk
    13921 avadlnviem tpavtvetvp sekekeyqpg dkpdtktaet sissleigsi qstvvqeseg
    13981 vyrpdekptk alaetsirpe ehvmiseiqt adypsdfree lkyvqesgtv svqlteakmi
    14041 qetlthdree rmeemvkpee rmvdtsydvn rsvevfqtts vekeadlrvf empeshrgkt
    14101 vpthpvvsle vemtqpednl gevkkevpss gtakvehvsl qetivgetvv aenlapvekd
    14161 kapetkvaei amneveslht tmvvvseket eytqvsdvkg ayastefttq vapmleevrs
    14221 hspteeylpd qkptsgiaqp shvplesitv avqelaeked lyktdvrpdq ktanvaltel
    14281 tsisipevmq vitassaetl kketkmkeek grveveelss tiisevisne tedvfttkat
    14341 pkdqkadfni sgreaaettq vtamteaedl avkrpeeqkg kpqvdeltpl tvshvetgea
    14401 ekelptaevp kektaqpnif grdvaetmqv ltvtsaeela apkapegqkg kpqveeltpl
    14461 svsqvvsqea esalpapeap terkaqpdmt grdiaetmev ltmtsadila edvkpteqkg
    14521 mpgleefvpl svsqtvstek edvlpspeap ttktaqpslt grdiaetmqv vmmtsekeli
    14581 talapethkg ipnveeltpl tvsqtvshea edvfektaap sevtakpafs grevaqtsem
    14641 mivtnvgele kqvlpdeqkg qaqldelmpl tvsqvisgev eeglpeaekp kektaqpgil
    14701 grdiaettqv ltvtsaeela apkapegqkg kpqveelapl tvsqvvseea egilpapeap
    14761 terkaqpdmt grdiaqtmev ltmtsvdtft edkkpdeqkg hpeleefvpl svtqtvsnem
    14821 edvlpspeap stkiaqpslt grdvaetmqi ltmtseeeli taqtpeqhkg kpnveeltpl
    14881 tvsqtvsqea eeilekaaap tevtakpafs grevaqtsei mivtnvgele kqvlpdeqkg
    14941 qpqldeltpl tisqiisgev eeglpeaekp kektaqpgil grdiaettqv ltvtsaeele
    15001 apkapegqkg kpqveelapl tvsqvvsqea egilpvpeap terkaqadlt grdvaemmev
    15061 ltmasvdtlt edkkpaeqkg vpgleefvpl svsqtvstek eevlpspeap ttkvaeaslt
    15121 grdiaetmqv vtmtseeeli sarvpeqhkg ifnveeltsl tvsqtishet eeilekaaal
    15181 gevtakpaft grevaqtset livtnvgele kqilpeeqkg kprvdemtsi tvsqvvsgev
    15241 eerlpeaekp kektaqpsml grdiaettqv ltmtsaeela apkapeeqkg kpqveelapl
    15301 tvsqvvsqea egvlpvpeap terqaqpdlt grdvaqitvv msmasadvmi ddkkpeeqkg
    15361 vpgveefvpl svsqtvstek edvlpspeap ttkmaqpslt grdiaetmqv vtmtseeeli
    15421 talapethkg kpnveeltsl tvsqtisqet evileksaap seatakpaft greiaqtsei
    15481 lavlnvgele khvlpeeqkg kpkleeltpl tvsqiisgea eeglptaevp kektaepsml
    15541 grdiaettqv ltmasaeelm apkapeeqkg kpqveelapl tisqvvsqea egvlpvpeap
    15601 terkaqpdlt grdvaqttvv msmanvdimi eekkpeeqrg ipgleefvpl svsqtvstek
    15661 edvlpspevp ttkmaqpslt ghdvaetlqv ltmvseeqlt tkapeeqkgk pnveeltslt
    15721 vsqtvsheae dilekavaps eatakpaflg revaetsqvl tvttvgelqk dvlpeeqkgk
    15781 pqleelmplt vsqivsgeae erlpeaekpk ektaepsmlg rdiaettqvl tmasaeella
    15841 pkvpeeqkgk pqveelaplt vsqvvsqeae gtlpipeapt ertaqpdltg rdvaetmevl
    15901 tmasldtlie dkkpaaqtga pgleefipls vsqmvsteke dvlpspevpt ekmaqpslig
    15961 rdvaetmqvl tmisameltt ghapdqqkgv pnveeltslt vsqtvshete eilekavaps
    16021 tvtakaalsg revaetsqvl tvsnveefek silpegqkgk prldeltsit vsqvlsnele
    16081 etlpspekps ekiaqpqmig revaeqtevl tvtnveelkk lekpegqtgk pdveemsfit
    16141 vsevfsteme kempspevpk ertalpqlss ievaettevl tmisaeellk skapeeqkvq
    16201 pkldeltplt vshvvseeae ealptaeipk ektaqpsllg rdvaetsqvl tmasaeella
    16261 akvpeeqkgk pqieelaplt vsqvvsqeae ealpsaevpv ereaqpsmts rdvaqtmlvt
    16321 smsnvdimae skipeeqkgv pgleefvsls vsqtvsteke dvlpspevpt ekmaqpslig
    16381 rdvaettqil tmisateltt drapeqqkgv pnveeltslt vsqavshete eilekleapg
    16441 tvtakaalsg revaetsqvl tvsnveefek svlpeeqkgk prldeltsit vsqvvsnele
    16501 etlpspekps ekvaqpqmig revaektevl tvsnveemqk lekpegqkgi pdveemsfit
    16561 vsevvsteae kelpapeapk erkalpqlsg ievaeksevl tmtmteefak pkapeeqkgk
    16621 pqleellsls vsevalgeve kglptpdkpt eetakpsllg iqvaeksqvi assitgelqe
    16681 sikpdtkqii peqipfesie qsqtltqere stfvvekttt sataevsfrv seslevtqvt
    16741 atekeskevi kgvakeasaq advierkval ktevqpedma sefkvkkpeg ktakgvdeiq
    16801 qgvivtehlt ahefeselpe svlpfaklar vsiqadhleh ivdvtektve qeiieevkev
    16861 kptkkvikke kpvkakdevt eevveeiiie epekqeakpi veeeetviit ehdvrkapkk
    16921 kkevvikeee eifetietpt hkiirkkirp mkkgekevvv eeiiqkpree tvemeeqqiv
    16981 evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk akptveeekk kvkvtkvdtk
    17041 kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii eeviqktiee akameeqeiv
    17101 evietptkkv ikkktttiee gkapeekvee iviekpkevi eeeeeiieti etptkrivrk
    17161 kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv ikkkkatike gkapeeevee
    17221 iviekpikek akkkilpkeg veitevitet sveelieeel kpeeakpsee veeekvkite
    17281 vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp tvtekegiev tevisevape
    17341 klpeeakpkk kkateikete ekvitkkvkv keapkeavpg eeeqpaeevd elvpekpkkt
    17401 kakkevtpqd sivttevvpi tkeeelevek peeekatvve ekeekakvee kkvtvkkapk
    17461 kklkpteaee eqveevveei tpeepkkrka kkavipkeei qttevipgit aeeipeeekp
    17521 eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge vieekpkvke ipeekpkeev
    17581 pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee vkpeeekpev kpeeekpkee
    17641 kpkkkkpaek kpkiseeerp edkveeipek kpeevktree iipkdtvqtt evipetvaee
    17701 iptkkpkerk avpveekeek veitevpvek apekkeekvp eekpteaape kpkeakvmkk
    17761 kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke kkplpeslqk lvpqkierke
    17821 mkptklkyse paetvqfasi klkkvavpkk keadetkfpk imlrsrmtfv gdyppelqip
    17881 kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev tqlekldlef eelkkvplek
    17941 iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe kvklkkvpek kpeeveeplk
    18001 kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek yepeeyekpe epapepvpkp
    18061 yekepkkkpd qeveqiplkk gkpkspepde epevkfript kekpeeepeq itlkpwkkek
    18121 peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk ekpkeekeee vtvkkpeeek
    18181 peevaeevtl kkepekpvee apaevtikkp vveekeveee etitevilkk kpekpeevve
    18241 eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi kkpkkksipe iteevteeit
    18301 lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv eekkeeevpe evtlkpkkpe
    18361 ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee aadeitlkkp vvvekeeeei
    18421 teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape evteeiaikk pkkkkpvkee
    18481 aadevtikkp vveekkeeet peevtlkprk pkekpvpeev keevtlkkpv peekekvpee
    18541 vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit eeitlgkkkk pekkpvpeei
    18601 teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea adevtikkpv vvekeeeeap
    18661 aeitlkrrpk ekpveeitee vteeitikkp tpeekekape evsekvevkk pkkkkpvvee
    18721 aadevtikkv vveekkeeea peevtlkprp kkksvteeev aevtltkpkp ieeekkpees
    18781 eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti rkkkkpekkp kvteeveeta
    18841 vtikkarape kpteeeeitt dvqikkkkpq kkveevaade ltikkieeve qpeeeeevqe
    18901 ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei tetenvtfrp rttktkedve
    18961 qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet iriiqevedd sgpiieeiie
    19021 sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie egdvklklkr erkysieead
    19081 eslalklksk rrvstydeee atlsitreed iseeveieyv vrdgdtmfsi csyvaetdea
    19141 inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln evhytlylqr klhekidklp
    19201 vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv prpqitwfrq taiikpspdf
    19261 qmyydddnva tliirevfpe dagtftcvak naagfasstt eliveaplsd hgsdltgpsr
    19321 kslsressla dilegipptf srkpkakyvd egedvilecr lvavpepeit wyykdtqitt
    19381 keniiiates dmhmycsvik itkvqkkqeg kytivarnre geatieipmk vktgkseppe
    19441 ileplqsyvv regetvvlst qivgnpapkv twfkngkplk gltpkqdghv ntltliqpqv
    19501 sdtgeysvva tndlgtaetk atltveinlk pslainfqqh tfehfsvtke ipsgapeppl
    19561 fterfqeltv pekgtfklva kvtgnpvpev twlrnnkple kspnitetyd genivleirn
    19621 ansevdtgdy kcvasnpvgk ashgarvtvd vdkvtftktl ekevvvdeyk tleltcetsh
    19681 tvstkwwhnd keisgmdhre iieegrvhkl vikktnptde gtykctvknq stssnvtvka
    19741 tkpefvkklq dcevkerdva ileveitsqt vdvkwykdge qlgpgkekle fvkegtvrkl
    19801 lirstsvhde geytcslldq ectaevtvie lppeiitkmq dvtiargera tfdieltkgd
    19861 alvrwfkdgq elqfsehvrl sidgkrqklk iydtesedag vyscevgqqk ssakltveep
    19921 avefitklpd vtlvplnsda vfvielsqdv pvtwmrktqv ikespkytii degkvkkliv
    19981 kkcttedise ytamvanvkt ssrlrvevie tppkispdtp rkykvrkded vdivvkfsgt
    20041 pkpedewtvn grvvtkskra iptideesav ltirkiqeed vgdytlklvn svgetsiein
    20101 vvivqvpsap gapeplettn dsvtlhwkkp dsdgnspive yilehqekte ttwtkiteti
    20161 tetthkvtkl ttnkeytfrv tavneagpge tsptspyvki skpstaeppv ileplksivi
    20221 glgetvtlsc viggtpapei twlrndeife dtsityenhi tkytirette tssakftvka
    20281 knnvgsaett celkiqespk iiydenltnq klpmngqwki eiqitgfpkp evtwtkngke
    20341 vdekrvsihr eertstvsis slvredtaty makaenqags ssvdlhlkvi dkpskpqgpv
    20401 vfkeirqdrv tlewrppadd ggielekyti ekcepgktwv kvvdidkeve sfcvhklqqn
    20461 aeykfriiar navgaseple sdtvkmrtsf eppgaprgpl evsgmtktsf tikwqppend
    20521 ggtpitdyii eikeeskktw qkigstkhet thfsvsdlkt dvpynfrita knsvgsgppy
    20581 vaeepiapgr rikitkltes smyallgifp ptkivisktp psspqhvhvt nitsksatls
    20641 wsppastggt eltgyviekr pligkgarwt kvvtldattl qycvenlkes eflfrifaen
    20701 nqglsvpans epvtlkthas vpspptaple irqiaantvv iswgrpdsdg gaplegykva
    20761 irdtkrtmwm evarvnteiq klnirdlqen hmylirifar neiglsdple seepfkiipa
    20821 seltvvepia eatekgetas isfstentss wlrehnmdad ihsyararll rqdeyffriw
    20881 hyakklfe
//
LOCUS       XP_034182923           20668 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X17 [Osmia lignaria lignaria].
ACCESSION   XP_034182923
VERSION     XP_034182923.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327032.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182923.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..20668
                     /product="sallimus isoform X17"
                     /calculated_mol_wt=2349230
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1318..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18906..18958
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(18911,18913,18919..18920,18937..18939,18952,18954,
                     18956..18957)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          18994..19084
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19011..19015
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19024..19028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19050..19054
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19064..19069
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19077..19080
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19118..19211
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19135..19139
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19148..19152
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19173..19181
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19191..19196
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19204..19207
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19219..19305
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19236..19240
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19249..19253
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19271..19275
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19285..19290
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19298..19301
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19339..19429
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19356..19360
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19369..19373
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19394..19398
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19409..19414
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19422..19425
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19442..19519
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          19452..19456
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19463..19467
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19488..19492
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19502..19507
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19523..19608
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19540..19544
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19552..19556
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19578..19582
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19592..19597
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19601..19604
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19613..19697
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19630..19634
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19642..19646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19667..19671
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19681..19686
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19690..19693
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19803..19883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19811..19815
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19824..19828
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19849..19853
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19863..19868
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19876..19879
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19887..19978
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(19887,19952,19967)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(19968..19969,19971..19972)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          19989..20075
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          20006..20010
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20019..20023
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20041..20045
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20055..20060
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20068..20071
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20099..20169
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          20099..20102
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          20111..20115
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20135..20139
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20149..20154
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20162..20165
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20173..20265
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20173,20239,20254)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <20243..20558
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(20255..20256,20258..20259)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..20668
                     /gene="sls"
                     /coded_by="XM_034327032.2:745..62751"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 iketteapaq qhhititqht tkhdlpqqqs iesdteviee yttkfrrgst edataavktk
    13501 ktivrkkpkt tgeedivvvi eeelddikpk ipsipkkqfm pieevttvsd veeivsarid
    13561 eaeeivpkee ekvevtitev qdtdkkkkvt kkqfvrrrgk trqvteeitv eeegkapett
    13621 vvtkpveiie edieerpkie keeyvveevp eektiplkpr kkltptvqqv eeqvtvteei
    13681 tkegkprktt kkkvitrkgk eqqvtevvtv eeqgkapits vtegpveevv eeiiqklpap
    13741 kyakpsevee vreqvtvtee vtrlgkpkkt tkkkiikrkg keqqvtevvt veeegkaptt
    13801 tvtegpveev vieeikelpi pefvkpteve evreqvtvte eltkmgkpkk tikkkiikrk
    13861 gkeqqvtevv tveeqgkapv ttvkegpvee vveetitpll apeyakpsev eevreqvtvt
    13921 qevtklgkpk kttkkkiikr kgkeqqvtei vtveeegkap kttvtegpve eiieeeikpe
    13981 fikpreveev reqvtvtqev tregkpkkti kkkiikkkgk eqqvtevvtv eeqgkapvtt
    14041 vtegpveevv detvkalpap eyakpsevee vreqvtvtqe vtklgkpkkt tkkkiikrkg
    14101 keqqvteivt veeegkapkt tvtegpveei ieeeikpefi kpreveevre qvtvtqevtr
    14161 egkpkktikk kiikkkgkee qvtevvtvee qgkapvttvn egpveevvde tvkalpapey
    14221 aepseveevr eqvtvtqevt klgkpkkttk kkiikrkgke qqvteivtve eegkapkttv
    14281 tegpveeiie eeikpefikp reveevreqv tvtqevtreg kpkktikkki ikkkgkeeqv
    14341 tevvtveeqg kapvttvneg pveevvdetv kalpapeyae pseveevreq vtvtqevtkl
    14401 gkpkkttkkk iikrkgkeqq vteivtveee gkapkttvte gpveeiieee ikpefikpre
    14461 veevreqvtv tqevtregkp kktikkkiik kkgkeqqvte lvtveeqgka pvttvtegpv
    14521 eevvdetvka lpapeyakps eveevreqvt vtqevtklgk pkkttkkkii krkgkeqqvt
    14581 eivtveeegk apkttvtegp veeiieeeik pefikpreve evreqvtvtq evtregkpkk
    14641 tikkkiikkk gkeqqvtevv tveeqgkapv ttvtegpvee vvdetvkalp apeyakpsev
    14701 eevreqvtvt qevtklgkpk kttkkkiikr kgkeqqvtei vtveeegkap kttvmegpve
    14761 evvieeiqel pipefvkpte veevreqvtv teevtrlgkp kkttkkkiik rkgkeqqvte
    14821 ivtveeqgka pvttvtegpv eevvdetiks lpapeyakps eveevreqvt vtqevtklgk
    14881 pkkttkkkiv krkgkeqqvt eivtveeegk apkttvtegp veevveeevk pllapefvkp
    14941 seveevheqv tvtqevtreg kpmktikkki ikkkgkaqqv tevvtveeeg kvpvttvkeg
    15001 pveevvdetv kalpapkyak pseveevreq vtvtqeitkl gkpkkttkrk vikrkgkeqq
    15061 vtevvtveee gkapettvte gpveevleee ikplpgpefv kpseveevre qvtvteevtr
    15121 egkpkktikk kiikkkgkeq qvteivtvee qgkapvtsvt egpveevvde tikqlpapky
    15181 akpseveevr eqvtvtqemt klgkpkkttk kkiikrkgke qqvteivtve eegkapkttm
    15241 tegpveevve eeikplpale fvkpseveev reqvtvteev tregkpkkti kkkiikkkgk
    15301 eqqitevvtv eeqgkapvtt vtkapveeiv detikelpal efakpsevee vreqvtvtke
    15361 vtkegkpkkt ikrkvikkkg keqqvteivt veeegkapkt titegpveev veeeikplpa
    15421 lefvkpseve evreqvtvte evtregkpkk tikkkiikkk gkeqqvtevv tveeqgkapv
    15481 ttvqegpvee videtikslp apeyakpsev eeireqvtvt eeltklgkpk kttkkkitkr
    15541 kgpeqqitei vtveeegkap vttvtegpve evvkevlkpl pvpkvvkpse veeireqvtv
    15601 teemtkegkp kkiikkkvik rkgreqtvqe vvtveekgka pvttvtvgpt eeiveetvkt
    15661 lpapeyakps eveevreqvt vtqevtkegk pkkttkkkvi krkgkeqqvt evvtveeqgk
    15721 apvttvtegp veevveellk pliapeyvkp seveevreqv tvteeltkeg kpkkvikkkv
    15781 ikrrgreqqv kevvtveeqg kapvttvteg pteeiveeti kalpapedak pseveevreq
    15841 vtvtqevtke gkpkktikkk vtkrkgkeqq vtevvtveek gkapvttvte gpveeiveet
    15901 vkalpapelv kptevtevpe eltvtevvtk egktkkivkk kvpkpkkegi eapeeapeev
    15961 pveetkkiik lqrlkvskfe stkldiqets lgkpqfaqik lrktpvakrp skkdekfpri
    16021 llrsriiaie wppspknlkv dtfepielqn gilsrnvdea aalpkpkkkk ikriekeiak
    16081 lekldqefee lkkelplekl eepileekpe rkvkkkkkpe dkakegvkpq lmrisikeqk
    16141 pikpkiaepa tplfaqiklk kapakpkkkp eeevskfpki llrsritahe wppsvkypai
    16201 svlepnyiqn gilsrtmeea lklkkvkhkk mklpekelte lekldqefee lkkvplekve
    16261 eitpyerkpk pekpeeekpk klvigkgkrr paeeedletv qlkkipekip sapeeaekvp
    16321 kkkdevdkpk kekeekeype lkpfepydie apeveleere kpeypepekp kekkvpgkkp
    16381 kkerktpaqe teavpivpgv pkpkepeeee eikrriperp ppteepekik lkpwkkgekp
    16441 eeeekkpedy ikfvpkdddi vetvevtttv eetpdkkqrk ikkkktitkp kdekpkiiee
    16501 ttieeegfep vvtveefdvd kyeavpvesv ekaapveeik eeivtseiit eegtkktvrk
    16561 krvtkkregk ervteevtve eegkepitat ielhedtise iheqakhkrp tkpkadekla
    16621 edvtektveq qeiieevkev kptkkvikke kpvkakdevt eevveeiiie epekqeakpi
    16681 veeeetviit ehdvrkapkk kkevvikeee eifetietpt hkiirkkirp mkkgekevvv
    16741 eeiiqkpree tvemeeqqiv evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk
    16801 akptveeekk kvkvtkvdtk kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii
    16861 eeviqktiee akameeqeiv evietptkkv ikkktttiee gkapeekvee iviekpkevi
    16921 eeeeeiieti etptkrivrk kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv
    16981 ikkkkatike gkapeeevee iviekpikek akkkilpkeg veitevitet sveelieeel
    17041 kpeeakpsee veeekvkite vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp
    17101 tvtekegiev tevisevape klpeeakpkk kkateikete ekvitkkvkv keapkeavpg
    17161 eeeqpaeevd elvpekpkkt kakkevtpqd sivttevvpi tkeeelevek peeekatvve
    17221 ekeekakvee kkvtvkkapk kklkpteaee eqveevveei tpeepkkrka kkavipkeei
    17281 qttevipgit aeeipeeekp eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge
    17341 vieekpkvke ipeekpkeev pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee
    17401 vkpeeekpev kpeeekpkee kpkkkkpaek kpkiseeerp edkveeipek kpeevktree
    17461 iipkdtvqtt evipetvaee iptkkpkerk avpveekeek veitevpvek apekkeekvp
    17521 eekpteaape kpkeakvmkk kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke
    17581 kkplpeslqk lvpqkierke mkptklkyse paetvqfasi klkkvavpkk keadetkfpk
    17641 imlrsrmtfv gdyppelqip kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev
    17701 tqlekldlef eelkkvplek iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe
    17761 kvklkkvpek kpeeveeplk kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek
    17821 yepeeyekpe epapepvpkp yekepkkkpd qeveqiplkk gkpkspepde epevkfript
    17881 kekpeeepeq itlkpwkkek peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk
    17941 ekpkeekeee vtvkkpeeek peevaeevtl kkepekpvee apaevtikkp vveekeveee
    18001 etitevilkk kpekpeevve eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi
    18061 kkpkkksipe iteevteeit lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv
    18121 eekkeeevpe evtlkpkkpe ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee
    18181 aadeitlkkp vvvekeeeei teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape
    18241 evteeiaikk pkkkkpvkee aadevtikkp vveekkeeet peevtlkprk pkekpvpeev
    18301 keevtlkkpv peekekvpee vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit
    18361 eeitlgkkkk pekkpvpeei teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea
    18421 adevtikkpv vvekeeeeap aeitlkrrpk ekpveeitee vteeitikkp tpeekekape
    18481 evsekvevkk pkkkkpvvee aadevtikkv vveekkeeea peevtlkprp kkksvteeev
    18541 aevtltkpkp ieeekkpees eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti
    18601 rkkkkpekkp kvteeveeta vtikkarape kpteeeeitt dvqikkkkpq kkveevaade
    18661 ltikkieeve qpeeeeevqe ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei
    18721 tetenvtfrp rttktkedve qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet
    18781 iriiqevedd sgpiieeiie sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie
    18841 egdvklklkr erkysieead eslalklksk rrvstydeee atlsitreed iseeveieyv
    18901 vrdgdtmfsi csyvaetdea inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln
    18961 evhytlylqr klhekidklp vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv
    19021 prpqitwfrq taiikpspdf qmyydddnva tliirevfpe dagtftcvak naagfasstt
    19081 eliveaplsd hgsdltgpsr kslsressla dilegipptf srkpkakyvd egedvilecr
    19141 lvavpepeit wyykdtqitt keniiiates dmhmycsvik itkvqkkqeg kytivarnre
    19201 geatieipmk vktgkseppe ileplqsyvv regetvvlst qivgnpapkv twfkngkplk
    19261 gltpkqdghv ntltliqpqv sdtgeysvva tndlgtaetk atltveinlk pslainfqqh
    19321 tfehfsvtke ipsgapeppl fterfqeltv pekgtfklva kvtgnpvpev twlrnnkple
    19381 kspnitetyd genivleirn ansevdtgdy kcvasnpvgk ashgarvtvd vdkvtftktl
    19441 ekevvvdeyk tleltcetsh tvstkwwhnd keisgmdhre iieegrvhkl vikktnptde
    19501 gtykctvknq stssnvtvka tkpefvkklq dcevkerdva ileveitsqt vdvkwykdge
    19561 qlgpgkekle fvkegtvrkl lirstsvhde geytcslldq ectaevtvie lppeiitkmq
    19621 dvtiargera tfdieltkgd alvrwfkdgq elqfsehvrl sidgkrqklk iydtesedag
    19681 vyscevgqqk ssakltveep avefitklpd vtlvplnsda vfvielsqdv pvtwmrktqv
    19741 ikespkytii degkvkkliv kkcttedise ytamvanvkt ssrlrvevie tppkispdtp
    19801 rkykvrkded vdivvkfsgt pkpedewtvn grvvtkskra iptideesav ltirkiqeed
    19861 vgdytlklvn svgetsiein vvivqvpsap gapeplettn dsvtlhwkkp dsdgnspive
    19921 yilehqekte ttwtkiteti tetthkvtkl ttnkeytfrv tavneagpge tsptspyvki
    19981 skpstaeppv ileplksivi glgetvtlsc viggtpapei twlrndeife dtsityenhi
    20041 tkytirette tssakftvka knnvgsaett celkiqespk iiydenltnq klpmngqwki
    20101 eiqitgfpkp evtwtkngke vdekrvsihr eertstvsis slvredtaty makaenqags
    20161 ssvdlhlkvi dkpskpqgpv vfkeirqdrv tlewrppadd ggielekyti ekcepgktwv
    20221 kvvdidkeve sfcvhklqqn aeykfriiar navgaseple sdtvkmrtsf eppgaprgpl
    20281 evsgmtktsf tikwqppend ggtpitdyii eikeeskktw qkigstkhet thfsvsdlkt
    20341 dvpynfrita knsvgsgppy vaeepiapgr rikitkltes smyallgifp ptkivisktp
    20401 psspqhvhvt nitsksatls wsppastggt eltgyviekr pligkgarwt kvvtldattl
    20461 qycvenlkes eflfrifaen nqglsvpans epvtlkthas vpspptaple irqiaantvv
    20521 iswgrpdsdg gaplegykva irdtkrtmwm evarvnteiq klnirdlqen hmylirifar
    20581 neiglsdple seepfkiipa seltvvepia eatekgetas isfstentss wlrehnmdad
    20641 ihsyararll rqdeyffriw hyakklfe
//
LOCUS       XP_034182932           20555 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X18 [Osmia lignaria lignaria].
ACCESSION   XP_034182932
VERSION     XP_034182932.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327041.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182932.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20555
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..20555
                     /product="sallimus isoform X18"
                     /calculated_mol_wt=2312209
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <1804..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8270..8274
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9408..9499
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9425..9429
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9438..9442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9465..9469
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9479..9484
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9492..9495
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <12987..>13139
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          18793..18845
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(18798,18800,18806..18807,18824..18826,18839,18841,
                     18843..18844)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          18881..18971
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          18898..18902
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          18911..18915
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          18937..18941
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          18951..18956
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          18964..18967
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19005..19098
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19022..19026
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19035..19039
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19060..19068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19078..19083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19091..19094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19106..19192
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19123..19127
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19136..19140
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19158..19162
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19172..19177
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19185..19188
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19226..19316
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19243..19247
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19256..19260
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19281..19285
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19296..19301
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19309..19312
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19329..19406
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          19339..19343
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19350..19354
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19375..19379
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19389..19394
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19410..19495
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19427..19431
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19439..19443
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19465..19469
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19479..19484
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19488..19491
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19500..19584
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19517..19521
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19529..19533
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19554..19558
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19568..19573
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19577..19580
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19690..19770
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19698..19702
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19711..19715
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19736..19740
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19750..19755
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19763..19766
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19774..19865
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(19774,19839,19854)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(19855..19856,19858..19859)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          19876..19962
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          19893..19897
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19906..19910
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          19928..19932
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          19942..19947
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          19955..19958
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          19986..20056
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          19986..19989
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          19998..20002
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          20022..20026
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          20036..20041
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          20049..20052
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          20060..20152
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(20060,20126,20141)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <20130..20445
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(20142..20143,20145..20146)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..20555
                     /gene="sls"
                     /coded_by="XM_034327041.2:758..62425"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprriv gtkeyrkpew vthmeemqea lratqtvprf iqeitdvyvk
     9421 egdtavfecs ysgnptpdvv wykndkmlms tqnlkvriyd ddkktvltik hateeddgay
     9481 vckitsetgl atskaklhvt dvtdekkfye eeeeveeqti evkpkekpqk kklrkakevk
     9541 ekvktervkv dkkevrkekl vpkeqpeekk ivdieetqil estdiieekp tevsqakkiv
     9601 piqepvltea taslkkidde kpkdmvpkpe erakrvieer egivvvsevt tedmapdftv
     9661 eskvdraqit seslqtvtvs evhtetsvqe vrraeakqrk akktvvteee ifeerpkevv
     9721 veekpkkkkk tekvkvreev tieeiverqr eniareveei memlhakefg pgeaplrela
     9781 tigylvrqgv svneinesly rtenfpalkt peaqnalvql vereghgpli tqvlteettt
     9841 desvvaatvg frafmrmiel qhvtveevlt hfapeafrpr awevteatev eteervterv
     9901 dvvrktevhf merrderkav rtqdirdike yedtrqthtt lrarkdrkpr eetkedrdeg
     9961 vqvveieeie dteqekmkkk rkdveeieee ietvivetds kgrlaqkkkk rddleekedv
    10021 eeeeeiiekv rkelkpkrpk igrdevelee ieefdvtedk smpsimvnvp sqrhqvvpld
    10081 rtteqapgkp elekakltvd titaltehiv pvqekeisdi srikpierka slsispvepy
    10141 sttettvqas tgefadtfkp tsyeatpgii psegvqvset lvsdagvtsl svskqeigrt
    10201 aevsvtlqea ttvyetmvre kevptedfvt pqtvkaedti lpqlslsvye vhegltedkl
    10261 eplktvatkp rvnvtavepl vveevraedk pgkyypeliv ptevatqsvi sqrqrvteem
    10321 hapekegeyi pgrlppsqva qigisygnet avvqqnvvqe segvfisekk tetfeattnv
    10381 tvlegvsvst vqtqdtetsl tieetkkava dlnviemtpa vtvetvpsek ekeyqpgdkp
    10441 dtktaetsis sleigsiqst vvqesegvyr pdekptkala etsirpeehv miseiqtady
    10501 psdfreelky vqesgtvsvq lteakmiqet lthdreerme emvkpeermv dtsydvnrsv
    10561 evfqttsvek eadlrvfemp eshrgktvpt hpvvslevem tqpednlgev kkevpssgta
    10621 kvehvslqet ivgetvvaen lapvekdkap etkvaeiamn eveslhttmv vvseketeyt
    10681 qvsdvkgaya stefttqvap mleevrshsp teeylpdqkp tsgiaqpshv plesitvavq
    10741 elaekedlyk tdvrpdqkta nvalteltsi sipevmqvit assaetlkke tkmkeekgrv
    10801 eveelsstii sevisneted vfttkatpkd qkadfnisgr eaaettqvta mteaedlavk
    10861 rpeeqkgkpq vdeltpltvs hvetgeaeke lptaevpkek taqpnifgrd vaetmqvltv
    10921 tsaeelaapk apegqkgkpq veeltplsvs qvvsqeaesa lpapeapter kaqpdmtgrd
    10981 iaetmevltm tsadilaedv kpteqkgmpg leefvplsvs qtvstekedv lpspeapttk
    11041 taqpsltgrd iaetmqvvmm tsekelital apethkgipn veeltpltvs qtvsheaedv
    11101 fektaapsev takpafsgre vaqtsemmiv tnvgelekqv lpdeqkgqaq ldelmpltvs
    11161 qvisgeveeg lpeaekpkek taqpgilgrd iaettqvltv tsaeelaapk apegqkgkpq
    11221 veelapltvs qvvseeaegi lpapeapter kaqpdmtgrd iaqtmevltm tsvdtftedk
    11281 kpdeqkghpe leefvplsvt qtvsnemedv lpspeapstk iaqpsltgrd vaetmqiltm
    11341 tseeelitaq tpeqhkgkpn veeltpltvs qtvsqeaeei lekaaaptev takpafsgre
    11401 vaqtseimiv tnvgelekqv lpdeqkgqpq ldeltpltis qiisgeveeg lpeaekpkek
    11461 taqpgilgrd iaettqvltv tsaeeleapk apegqkgkpq veelapltvs qvvsqeaegi
    11521 lpvpeapter kaqadltgrd vaemmevltm asvdtltedk kpaeqkgvpg leefvplsvs
    11581 qtvstekeev lpspeapttk vaeasltgrd iaetmqvvtm tseeelisar vpeqhkgifn
    11641 veeltsltvs qtisheteei lekaaalgev takpaftgre vaqtsetliv tnvgelekqi
    11701 lpeeqkgkpr vdemtsitvs qvvsgeveer lpeaekpkek taqpsmlgrd iaettqvltm
    11761 tsaeelaapk apeeqkgkpq veelapltvs qvvsqeaegv lpvpeapter qaqpdltgrd
    11821 vaqitvvmsm asadvmiddk kpeeqkgvpg veefvplsvs qtvstekedv lpspeapttk
    11881 maqpsltgrd iaetmqvvtm tseeelital apethkgkpn veeltsltvs qtisqetevi
    11941 leksaapsea takpaftgre iaqtseilav lnvgelekhv lpeeqkgkpk leeltpltvs
    12001 qiisgeaeeg lptaevpkek taepsmlgrd iaettqvltm asaeelmapk apeeqkgkpq
    12061 veelapltis qvvsqeaegv lpvpeapter kaqpdltgrd vaqttvvmsm anvdimieek
    12121 kpeeqrgipg leefvplsvs qtvstekedv lpspevpttk maqpsltghd vaetlqvltm
    12181 vseeqlttka peeqkgkpnv eeltsltvsq tvsheaedil ekavapseat akpaflgrev
    12241 aetsqvltvt tvgelqkdvl peeqkgkpql eelmpltvsq ivsgeaeerl peaekpkekt
    12301 aepsmlgrdi aettqvltma saeellapkv peeqkgkpqv eelapltvsq vvsqeaegtl
    12361 pipeaptert aqpdltgrdv aetmevltma sldtliedkk paaqtgapgl eefiplsvsq
    12421 mvstekedvl pspevptekm aqpsligrdv aetmqvltmi samelttgha pdqqkgvpnv
    12481 eeltsltvsq tvsheteeil ekavapstvt akaalsgrev aetsqvltvs nveefeksil
    12541 pegqkgkprl deltsitvsq vlsneleetl pspekpseki aqpqmigrev aeqtevltvt
    12601 nveelkklek pegqtgkpdv eemsfitvse vfstemekem pspevpkert alpqlssiev
    12661 aettevltmi saeellkska peeqkvqpkl deltpltvsh vvseeaeeal ptaeipkekt
    12721 aqpsllgrdv aetsqvltma saeellaakv peeqkgkpqi eelapltvsq vvsqeaeeal
    12781 psaevpvere aqpsmtsrdv aqtmlvtsms nvdimaeski peeqkgvpgl eefvslsvsq
    12841 tvstekedvl pspevptekm aqpsligrdv aettqiltmi satelttdra peqqkgvpnv
    12901 eeltsltvsq avsheteeil ekleapgtvt akaalsgrev aetsqvltvs nveefeksvl
    12961 peeqkgkprl deltsitvsq vvsneleetl pspekpsekv aqpqmigrev aektevltvs
    13021 nveemqklek pegqkgipdv eemsfitvse vvsteaekel papeapkerk alpqlsgiev
    13081 aeksevltmt mteefakpka peeqkgkpql eellslsvse valgevekgl ptpdkpteet
    13141 akpsllgiqv aeksqviass itgelqesik pdtkqiipeq ipfesieqsq tltqerestf
    13201 vvektttsat aevsfrvses levtqvtate keskevikgv akeasaqadv ierkvalkte
    13261 vqpedmasef kvkkpegkta kgvdeiqqgv ivtehltahe feselpesvl pfaklarvsi
    13321 qadhlehive tteapaqqhh ititqhttkh dlpqqqsies dtevieeytt kfrrgsteda
    13381 taavktkkti vrkkpkttge edivvvieee lddikpkips ipkkqfmpie evttvsdvee
    13441 ivsarideae eivpkeeekv evtitevqdt dkkkkvtkkq fvrrrgktrq vteeitveee
    13501 gkapettvvt kpveiieedi eerpkiekee yvveevpeek tiplkprkkl tptvqqveeq
    13561 vtvteeitke gkprkttkkk vitrkgkeqq vtevvtveeq gkapitsvte gpveevveei
    13621 iqklpapkya kpseveevre qvtvteevtr lgkpkkttkk kiikrkgkeq qvtevvtvee
    13681 egkaptttvt egpveevvie eikelpipef vkpteveevr eqvtvteelt kmgkpkktik
    13741 kkiikrkgke qqvtevvtve eqgkapvttv kegpveevve etitpllape yakpseveev
    13801 reqvtvtqev tklgkpkktt kkkiikrkgk eqqvteivtv eeegkapktt vtegpveeii
    13861 eeeikpefik preveevreq vtvtqevtre gkpkktikkk iikkkgkeqq vtevvtveeq
    13921 gkapvttvte gpveevvdet vkalpapeya kpseveevre qvtvtqevtk lgkpkkttkk
    13981 kiikrkgkeq qvteivtvee egkapkttvt egpveeiiee eikpefikpr eveevreqvt
    14041 vtqevtregk pkktikkkii kkkgkeeqvt evvtveeqgk apvttvnegp veevvdetvk
    14101 alpapeyaep seveevreqv tvtqevtklg kpkkttkkki ikrkgkeqqv teivtveeeg
    14161 kapkttvteg pveeiieeei kpefikprev eevreqvtvt qevtregkpk ktikkkiikk
    14221 kgkeeqvtev vtveeqgkap vttvnegpve evvdetvkal papeyaepse veevreqvtv
    14281 tqevtklgkp kkttkkkiik rkgkeqqvte ivtveeegka pkttvtegpv eeiieeeikp
    14341 efikprevee vreqvtvtqe vtregkpkkt ikkkiikkkg keqqvtelvt veeqgkapvt
    14401 tvtegpveev vdetvkalpa peyakpseve evreqvtvtq evtklgkpkk ttkkkiikrk
    14461 gkeqqvteiv tveeegkapk ttvtegpvee iieeeikpef ikpreveevr eqvtvtqevt
    14521 regkpkktik kkiikkkgke qqvtevvtve eqgkapvttv tegpveevvd etvkalpape
    14581 yakpseveev reqvtvtqev tklgkpkktt kkkiikrkgk eqqvteivtv eeegkapktt
    14641 vmegpveevv ieeiqelpip efvkptevee vreqvtvtee vtrlgkpkkt tkkkiikrkg
    14701 keqqvteivt veeqgkapvt tvtegpveev vdetikslpa peyakpseve evreqvtvtq
    14761 evtklgkpkk ttkkkivkrk gkeqqvteiv tveeegkapk ttvtegpvee vveeevkpll
    14821 apefvkpsev eevheqvtvt qevtregkpm ktikkkiikk kgkaqqvtev vtveeegkvp
    14881 vttvkegpve evvdetvkal papkyakpse veevreqvtv tqeitklgkp kkttkrkvik
    14941 rkgkeqqvte vvtveeegka pettvtegpv eevleeeikp lpgpefvkps eveevreqvt
    15001 vteevtregk pkktikkkii kkkgkeqqvt eivtveeqgk apvtsvtegp veevvdetik
    15061 qlpapkyakp seveevreqv tvtqemtklg kpkkttkkki ikrkgkeqqv teivtveeeg
    15121 kapkttmteg pveevveeei kplpalefvk pseveevreq vtvteevtre gkpkktikkk
    15181 iikkkgkeqq itevvtveeq gkapvttvtk apveeivdet ikelpalefa kpseveevre
    15241 qvtvtkevtk egkpkktikr kvikkkgkeq qvteivtvee egkapkttit egpveevvee
    15301 eikplpalef vkpseveevr eqvtvteevt regkpkktik kkiikkkgke qqvtevvtve
    15361 eqgkapvttv qegpveevid etikslpape yakpseveei reqvtvteel tklgkpkktt
    15421 kkkitkrkgp eqqiteivtv eeegkapvtt vtegpveevv kevlkplpvp kvvkpsevee
    15481 ireqvtvtee mtkegkpkki ikkkvikrkg reqtvqevvt veekgkapvt tvtvgpteei
    15541 veetvktlpa peyakpseve evreqvtvtq evtkegkpkk ttkkkvikrk gkeqqvtevv
    15601 tveeqgkapv ttvtegpvee vveellkpli apeyvkpsev eevreqvtvt eeltkegkpk
    15661 kvikkkvikr rgreqqvkev vtveeqgkap vttvtegpte eiveetikal papedakpse
    15721 veevreqvtv tqevtkegkp kktikkkvtk rkgkeqqvte vvtveekgka pvttvtegpv
    15781 eeiveetvka lpapelvkpt evtevpeelt vtevvtkegk tkkivkkkvp kpkkegieap
    15841 eeapeevpve etkkiiklqr lkvskfestk ldiqetslgk pqfaqiklrk tpvakrpskk
    15901 dekfprillr sriiaiewpp spknlkvdtf epielqngil srnvdeaaal pkpkkkkikr
    15961 iekeiaklek ldqefeelkk elplekleep ileekperkv kkkkkpedka kegvkpqlmr
    16021 isikeqkpik pkiaepatpl faqiklkkap akpkkkpeee vskfpkillr sritahewpp
    16081 svkypaisvl epnyiqngil srtmeealkl kkvkhkkmkl pekeltelek ldqefeelkk
    16141 vplekveeit pyerkpkpek peeekpkklv igkgkrrpae eedletvqlk kipekipsap
    16201 eeaekvpkkk devdkpkkek eekeypelkp fepydieape veleerekpe ypepekpkek
    16261 kvpgkkpkke rktpaqetea vpivpgvpkp kepeeeeeik rriperpppt eepekiklkp
    16321 wkkgekpeee ekkpedyikf vpkdddivet vevtttveet pdkkqrkikk kktitkpkde
    16381 kpkiieetti eeegfepvvt veefdvdkye avpvesveka apveeikeei vtseiiteeg
    16441 tkktvrkkrv tkkregkerv teevtveeeg kepitatiel hedtiseihe qakhkrptkp
    16501 kadeklaedv tektveqqei ieevkevkpt kkvikkekpv kakdevteev veeiiieepe
    16561 kqeakpivee eetviitehd vrkapkkkke vvikeeeeif etietpthki irkkirpmkk
    16621 gekevvveei iqkpreetve meeqqivevv etptkkivkk kkevvkddgv peelveeiii
    16681 ekpqkkkakp tveeekkkvk vtkvdtkkap ekpeevveee eeiietietp tkkiirkkpk
    16741 dkkdkiieev iqktieeaka meeqeivevi etptkkvikk ktttieegka peekveeivi
    16801 ekpkevieee eeiietietp tkrivrkkpk dkkdkiveev iqktieeakt mkeqeiievi
    16861 etptktvikk kkatikegka peeeveeivi ekpikekakk kilpkegvei tevitetsve
    16921 elieeelkpe eakpseevee ekvkitevai kkvpkkkkps kaveaeekee vveeikpekp
    16981 keekakptvt ekegievtev isevapeklp eeakpkkkka teiketeekv itkkvkvkea
    17041 pkeavpgeee qpaeevdelv pekpkktkak kevtpqdsiv ttevvpitke eelevekpee
    17101 ekatvveeke ekakveekkv tvkkapkkkl kpteaeeeqv eevveeitpe epkkrkakka
    17161 vipkeeiqtt evipgitaee ipeeekpeee kavpkeeekv eeeakvtevs vkkkerkkke
    17221 kpvtngevie ekpkvkeipe ekpkeevpqv peekekpeee kpkevkpkek kpkeekpkkp
    17281 ieekpeevkp eeekpevkpe eekpkeekpk kkkpaekkpk iseeerpedk veeipekkpe
    17341 evktreeiip kdtvqttevi petvaeeipt kkpkerkavp veekeekvei tevpvekape
    17401 kkeekvpeek pteaapekpk eakvmkkkkk kpakkdeiee wvepeyerpv lepmpekivw
    17461 epkkkkekkp lpeslqklvp qkierkemkp tklkysepae tvqfasiklk kvavpkkkea
    17521 detkfpkiml rsrmtfvgdy ppelqipkit yleenpvqtg ilsrnteeal kvlkrkvkkv
    17581 klsekevtql ekldlefeel kkvplekied ksvyerqpkk paekpeepkk vpigkgkvpe
    17641 eekvepekvk lkkvpekkpe eveeplkkpk kpepeeevkp ekkkkpkvkl ehdelkpldf
    17701 drpelekyep eeyekpeepa pepvpkpyek epkkkpdqev eqiplkkgkp kspepdeepe
    17761 vkfriptkek peeepeqitl kpwkkekpee ekekeipkke eekvpkppke epedvtvqrk
    17821 aktkkpkekp keekeeevtv kkpeeekpee vaeevtlkke pekpveeapa evtikkpvve
    17881 ekeveeeeti tevilkkkpe kpeevveeav vkkpkkkkpv keeevveevt vtkvvteeek
    17941 eeekvvikkp kkksipeite evteeitlkk peekapeevt eeikkpkkkk pvkeevaeei
    18001 tikkpvveek keeevpeevt lkpkkpeekp vieevteeit lkkpvpeevt eevaikkpkk
    18061 kkpveeeaad eitlkkpvvv ekeeeeitee vtlqkkkkpe kkpvpeeise eitlkkpvpk
    18121 eeekapeevt eeiaikkpkk kkpvkeeaad evtikkpvve ekkeeetpee vtlkprkpke
    18181 kpvpeevkee vtlkkpvpee kekvpeevte eiaikkpkkk kpveeeaade vtikkpvvve
    18241 keeeeiteei tlgkkkkpek kpvpeeitee itlkkpvpke eekapeevte kievkkpkkk
    18301 kpveeeaade vtikkpvvve keeeeapaei tlkrrpkekp veeiteevte eitikkptpe
    18361 ekekapeevs ekvevkkpkk kkpvveeaad evtikkvvve ekkeeeapee vtlkprpkkk
    18421 svteeevaev tltkpkpiee ekkpeeselt ikkkpkkkpv ieeeaaevti kkpvpvepde
    18481 apeeitirkk kkpekkpkvt eeveetavti kkarapekpt eeeeittdvq ikkkkpqkkv
    18541 eevaadelti kkieeveqpe eeeevqeftf krkppklppk pieeiyedvt lrklrpkrks
    18601 rpdikeitet envtfrprtt ktkedveqef kislntyeee dismsgkvrl kpkkkpltys
    18661 eetgeetiri iqeveddsgp iieeiiesde egkdeysiee ldtdemrlpl rrkkkkvpkp
    18721 ykvedieegd vklklkrerk ysieeadesl alklkskrrv stydeeeatl sitreedise
    18781 eveieyvvrd gdtmfsicsy vaetdeainl vegervyvid htnqdwwfvk khlteekgwv
    18841 paqyllnevh ytlylqrklh ekidklpvfe kpapgdkssa pkfveklqpi htpdgytiqf
    18901 ecqvegvprp qitwfrqtai ikpspdfqmy ydddnvatli irevfpedag tftcvaknaa
    18961 gfasstteli veaplsdhgs dltgpsrksl sressladil egipptfsrk pkakyvdege
    19021 dvilecrlva vpepeitwyy kdtqittken iiiatesdmh mycsvikitk vqkkqegkyt
    19081 ivarnregea tieipmkvkt gkseppeile plqsyvvreg etvvlstqiv gnpapkvtwf
    19141 kngkplkglt pkqdghvntl tliqpqvsdt geysvvatnd lgtaetkatl tveinlkpsl
    19201 ainfqqhtfe hfsvtkeips gapepplfte rfqeltvpek gtfklvakvt gnpvpevtwl
    19261 rnnkpleksp nitetydgen ivleirnans evdtgdykcv asnpvgkash garvtvdvdk
    19321 vtftktleke vvvdeyktle ltcetshtvs tkwwhndkei sgmdhreiie egrvhklvik
    19381 ktnptdegty kctvknqsts snvtvkatkp efvkklqdce vkerdvaile veitsqtvdv
    19441 kwykdgeqlg pgkeklefvk egtvrkllir stsvhdegey tcslldqect aevtvielpp
    19501 eiitkmqdvt iargeratfd ieltkgdalv rwfkdgqelq fsehvrlsid gkrqklkiyd
    19561 tesedagvys cevgqqkssa kltveepave fitklpdvtl vplnsdavfv ielsqdvpvt
    19621 wmrktqvike spkytiideg kvkklivkkc ttediseyta mvanvktssr lrvevietpp
    19681 kispdtprky kvrkdedvdi vvkfsgtpkp edewtvngrv vtkskraipt ideesavlti
    19741 rkiqeedvgd ytlklvnsvg etsieinvvi vqvpsapgap eplettndsv tlhwkkpdsd
    19801 gnspiveyil ehqektettw tkitetitet thkvtklttn keytfrvtav neagpgetsp
    19861 tspyvkiskp staeppvile plksiviglg etvtlscvig gtpapeitwl rndeifedts
    19921 ityenhitky tirettetss akftvkaknn vgsaettcel kiqespkiiy denltnqklp
    19981 mngqwkieiq itgfpkpevt wtkngkevde krvsihreer tstvsisslv redtatymak
    20041 aenqagsssv dlhlkvidkp skpqgpvvfk eirqdrvtle wrppaddggi elekytiekc
    20101 epgktwvkvv didkevesfc vhklqqnaey kfriiarnav gaseplesdt vkmrtsfepp
    20161 gaprgplevs gmtktsftik wqppendggt pitdyiieik eeskktwqki gstkhetthf
    20221 svsdlktdvp ynfritakns vgsgppyvae epiapgrrik itkltessmy allgifpptk
    20281 ivisktppss pqhvhvtnit sksatlswsp pastggtelt gyviekrpli gkgarwtkvv
    20341 tldattlqyc venlkesefl frifaennqg lsvpansepv tlkthasvps pptapleirq
    20401 iaantvvisw grpdsdggap legykvaird tkrtmwmeva rvnteiqkln irdlqenhmy
    20461 lirifarnei glsdplesee pfkiipasel tvvepiaeat ekgetasisf stentsswlr
    20521 ehnmdadihs yararllrqd eyffriwhya kklfe
//
LOCUS       XP_034182939           18911 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X19 [Osmia lignaria lignaria].
ACCESSION   XP_034182939
VERSION     XP_034182939.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327048.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182939.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18911
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..18911
                     /product="sallimus isoform X19"
                     /calculated_mol_wt=2139874
     Region          74..161
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          91..95
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          131..135
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          145..150
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          271..360
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          288..292
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          301..305
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          326..330
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          340..345
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          353..356
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..486
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          413..417
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          426..430
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          452..456
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          466..471
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          479..482
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          495..585
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          512..516
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          525..529
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          551..555
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          565..570
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          578..581
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..733
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          659..663
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          674..678
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          700..703
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          713..718
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          726..729
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          775..867
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          793..797
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          808..812
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          833..837
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          847..852
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          860..863
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          914..1006
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          932..936
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          947..951
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          972..976
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          986..991
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          999..1002
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1048..1140
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1066..1070
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1081..1085
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1107..1111
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1120..1125
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1133..1136
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1182..1274
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1200..1204
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1215..1219
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1240..1244
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1254..1259
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1267..1270
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1316..1407
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1333..1337
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1348..1352
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1374..1377
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1387..1392
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1400..1403
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1449..1539
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1467..1471
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1482..1486
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1507..1511
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1521..1526
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1534..1537
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1583..1674
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1600..1604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1611..1619
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1640..1644
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1654..1659
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1667..1670
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1716..1807
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1733..1737
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1748..1752
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1773..1777
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1787..1792
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1800..1803
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1849..1941
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1867..1871
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1882..1886
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1907..1911
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1921..1926
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1934..1937
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1983..2075
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2001..2005
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2014..2020
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2041..2045
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2055..2060
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2068..2071
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2115..2207
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2133..2137
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2146..2152
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2173..2177
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2187..2192
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2200..2203
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2248..2340
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2266..2270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2279..2285
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2306..2310
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2320..2325
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2333..2336
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2382..2474
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2415..2419
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2440..2444
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2454..2459
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2467..2470
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2606
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2547..2551
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2572..2576
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2586..2591
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2648..2740
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2666..2670
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2681..2685
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2706..2710
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2720..2725
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2733..2736
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2780..2870
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2797..2801
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2812..2816
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2837..2841
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2851..2856
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2864..2867
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <2947..3007
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2951..2955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3004..3007
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3055..3146
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3088..3092
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3113..3117
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3127..3132
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3156..3247
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3174..3178
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3189..3193
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3211..3218
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3228..3233
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3241..3244
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3289..3380
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3307..3311
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3322..3326
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3348
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3361..3366
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3374..3377
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3427..3518
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3460..3464
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3485..3489
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3499..3504
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3565..3656
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3582..3586
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3597..3601
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3622..3626
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3636..3641
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3649..3652
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3702..3792
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3719..3723
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3734..3738
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3759..3763
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3773..3778
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3786..3789
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3835..3924
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3852..3856
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3867..3871
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3892..3896
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3906..3911
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3919..3922
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3978..4068
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3996..4000
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4011..4015
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4033..4037
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4050..4055
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4063..4066
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4116..4204
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4133..4137
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4146..4150
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4170..4174
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4184..4189
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4197..4200
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4439..4529
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4456..4460
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4469..4473
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4494..4498
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4508..4513
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4521..4524
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4543..4632
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4560..4564
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4573..4577
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4598..4602
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4612..4617
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4625..4628
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4653..4742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4670..4674
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4683..4687
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4708..4712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4722..4727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4735..4738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4754..4841
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4768..4772
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4782..4786
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4807..4811
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4821..4826
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4834..4837
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <4918..5615
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          5425..>5834
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          7092..7181
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7109..7113
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7122..7126
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7147..7151
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7161..7166
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7191..7282
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7208..7212
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7221..7225
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7248..7252
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7262..7267
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7275..7278
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7297..7386
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7314..7318
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7327..7331
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7352..7356
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7366..7371
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7381..7384
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7399..7485
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7416..7420
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7429..7433
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7454..7458
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7468..7473
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7481..7484
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7502..7592
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7519..7523
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7532..7536
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7558..7562
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7577
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7585..7588
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7640..7726
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7659..7663
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7672..7676
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7698..7702
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7712..7717
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7764..7855
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7781..7785
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7794..7798
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7821..7825
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7835..7840
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7848..7851
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <11343..>11495
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          17149..17201
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(17154,17156,17162..17163,17180..17182,17195,17197,
                     17199..17200)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          17237..17327
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17254..17258
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17267..17271
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17293..17297
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17307..17312
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17320..17323
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17361..17454
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          17378..17382
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17391..17395
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17416..17424
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17434..17439
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17447..17450
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17462..17548
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17479..17483
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17492..17496
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17514..17518
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17528..17533
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17541..17544
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17582..17672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17599..17603
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17612..17616
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17637..17641
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17652..17657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17665..17668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17685..17762
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          17695..17699
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17706..17710
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17731..17735
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17745..17750
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17766..17851
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          17783..17787
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17795..17799
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17821..17825
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17835..17840
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17844..17847
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17856..17940
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17873..17877
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17885..17889
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17910..17914
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17924..17929
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17933..17936
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18046..18126
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          18054..18058
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          18067..18071
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          18092..18096
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          18106..18111
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          18119..18122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18130..18221
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(18130,18195,18210)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(18211..18212,18214..18215)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          18232..18318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          18249..18253
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          18262..18266
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          18284..18288
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          18298..18303
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          18311..18314
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18342..18412
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          18342..18345
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          18354..18358
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          18378..18382
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          18392..18397
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          18405..18408
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18416..18508
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(18416,18482,18497)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <18486..18801
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(18498..18499,18501..18502)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..18911
                     /gene="sls"
                     /coded_by="XM_034327048.2:537..57272"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mkrqtsqqqq qtmqqqqqtt tqqqqqqqyi qqrterqvtr qqitsqqrvq geivmqttpt
       61 vpaftgrpgd psppvfeqif knarfaqggn aifegrvrgn pkptvtwthk gaplleswki
      121 rmsyddktga vtlqinqigp gdegeytcsa knqygeaics vyiqpegfgp ppqqqiggyr
      181 keyaqtfqst eqksqtgsqs fqqrsyqqti dkrsyvngts tsiedfkvdt feyrllrete
      241 fresitrrfv gesdvqistv vdrslgpvap pqisqkprns kliegsdavf takisgnpkp
      301 rltwfkngqr iresqrvems hsnqqatlri rvalpedsgh ytllsenpqg ctvssaylai
      361 essdqvdqay qtpresvktp qvestgesds gkvlppnfvr tctdrdateg kmtrfdcrvt
      421 grpypevtwy ingqqvandl thkilvnesg nnslmitnvs radagvvtcv arnkagetsf
      481 qcnlnvieke qvvapkfver ftttnvkege pvvfmaravg tpvpritwqk dgvpitpgpd
      541 vristdgsga stldipcakl sdaawyqcta qnvagstatr arlfvetpkg aapeprrlnl
      601 prptkviepe papgpeviyl rhverakpyl pppeedrvyp pprfiiplrd vhqieggrih
      661 feariepvgd ptmrvewyvn graldassra tsifrfgfis ldlisiilqd sgeyvcrvvs
      721 stgvaesrat lsvtpratie qasqhpdslq yiqqledysk yqrqesveei ssqrpafirp
      781 lqdlgelqeg rnahfeaqlt pvsdptmkve wykdgrpita ssrittifnf gyvslnilhl
      841 raedagsytv ravnrlgeav ssaslrvyar tsvttdlgip eqqryieaae eleayqqamh
      901 qkyvqeqpep tsppefkspi kdqnsiregg fahfearlep vgdsdlrvew lkdgrpveas
      961 srittffnfg yvaltikyvt ihdvgiytcr aynrageaht taqlsviskn dviydsqhpt
     1021 glqkiqtled ssrysrqvqe etqvtqaprf lgtlkgtnki vegqrahfea rvepqsdltm
     1081 tiewyhngka itaanriqty hdfgyvaidi lqvrpedagt ytvvarnalg earlsatmvv
     1141 etrssvdtss mhrtsyektq rleeskfvep qyhieeisks kpifvqplsd pkpvsegkni
     1201 hlecrlepmg dptmrvewfq ngrpvtvgsr frtyydfgfv aldivhstil dsgeytvrat
     1261 nhlgtahtsa cvrvigksdi vtetqheqsl eqiqmledaa ryrktqqeev tvmqapqftr
     1321 plhnietvel tnvhlecrlq pvgdatmkve wfvngrpvkt ghrfrpsyef dyvaldilgv
     1381 ypedsgvytc qarnqlgeav tscsvrvhak kdlllesqhp egleriqyle dasrykrhel
     1441 vdevvkvkpr fvtapknqen lhegqhahfe cklepvtdsn lkvewykngr pvtighrfrp
     1501 ihdfgyvald vidliaedsg tytcravnlv gsdevscsls crstaqvltd tkneqgleqi
     1561 hyledrskyq rredveettt qapifttsln nveikegqra hfecrlipvs datmkvewfh
     1621 nnkpvkagsr fietnsfgfv aldimyaype dsgtytcrak niigeaitsa tanvhskksi
     1681 ytetqneetl qrlhhledts ryqrktttee ivtqapvftm pikdlkvaen qaahfearvi
     1741 pvgdsklkve wlrngvpiaa snrvttmhdf gyvalnmkyv npedsgtytc ravndlgeav
     1801 tsatlfvqsk aalqfesqhe salsklqale dtsryqrhee eeivvkekps ftvqlngptn
     1861 lvegqsahye criepypdpn mkvewfhngk plstghryrt tcdfgfasld vltvyaedsg
     1921 tytckatnkl gsaqssikld vksrasiire tqhesalkki qyleddsryk rvdqedliia
     1981 ekpkfgrplk niehlpegks ahleatltpv ndptmvvewf rdgrpipqgh kfkttydfgy
     2041 valdilyayp edsgtymcka rnavgeavtt cvisvdskqg lfldtldaqr lqkireletv
     2101 evkqevekev vhqkpvfltp lnnldhlkeg ehahlecrve pindpnlkie wfvngvavkt
     2161 ghrfrkthdf gyvaldilyt ypedsgtymc katnlageav ntctikvgsr rsilldtqhp
     2221 dglekirele aqghparlev eeppvtpprf vtelrgttev yegqtahfec qieplhdanl
     2281 rieffhngkp lpsasrfhvt fdfgyvaldi ghtvpedage ysvrainalg qcvssielrv
     2341 iprdniilds qrpegldkir eleaqqpwkr pdipepqtrq rpvftqplqn idaipegqta
     2401 hfecrlipvg dptlkvewfr neipletssr itkvhdfgyv sldinhvree degvymcras
     2461 nplgeavtta smkikskasi qldtqhpeaq rkiaqleadk psrpeepekv fdkpiftqll
     2521 tgptelwegq maryecrvvp vgdaslrfew yingvelkmg srfhvshdfg yvtldilkvi
     2581 tedsgvytck ainkageavs sislkvkars aidadsiqpd awqkiqlkea emnkvpemfv
     2641 dttpqqapvf tkhlesydkl vegqhvylea qvepradpnl rvewfkngis lqtgtrlrst
     2701 fdfglvtlsi nglrpddsai ytckatnllg eavstcslki vdrhwllgdt lhpdalpkid
     2761 aleqphvtsa eqpepiyeep vfithlnnve cvegdnvhfe cnvepskdpt mkiewfingk
     2821 plptgarfks tydfgyvald ithayeedsg vvivkatnsk gsaqtsgtlk ctskqsiylq
     2881 tqhpqgeagl ekvkeaeday lskyrrpdeg peheypkpiw tvplqpefkl gefeplhleg
     2941 qvepkddpnl kiewyfngkv lehasrfkmt sdfgfvtldl tdvyerdsgi ytckaynkag
     3001 eaftsttiyc stkeniiers qhpkgkegle aiqdleeslk rqegappese eghppvftsq
     3061 fenltnlseg eiahfeaslt ptgdqtmvve wfyngkvlea shrirtvyaf gmvvlevlgt
     3121 kiedsgtysc ratnkwgqae isvelqcvdk skgqkpkftt hiqsleglkd gqsahfectl
     3181 ipvgdpqmkv ewfhngqplr hssrfkmvsd fgfvvmdiag vmahdtgeyv ckasnkyged
     3241 ftkatlkcfg ksgvyldslq pdslarirel esfggeqptt pttpvaeppk fitqivditr
     3301 lvegqsahfe arltpvndpd lkvewyyngk klphghryrt fhdfgivild ilycyeensg
     3361 vyecrafnky gedttkatlk cfskaslile sqlpkgmegg lekiqtleds mirtkdekvv
     3421 eergkapmft vplsnidglr egesahfear liptddpklk vewfwngkpl rtgsrfrtfc
     3481 dfgfvileis piypedsgey scratneyge avttctmkct gkrsiilesq lpkgmestid
     3541 kiaeleglgn epgqvtpedd tgkppefvtt psdltltens lahfecrltp indssmrvew
     3601 fhngkpllag sriktihdfg fvilevancy qrdsglytck atnrhgeatv ncklqvrgrq
     3661 giilepqlpt nfktgtesiq kleealykrd eilteeetpn ppkftvelkd ieveegapsh
     3721 fdcrvepvgd stmridwfhn grsfatgsrv hqindfgfis ldmsytyard sgeyvcratn
     3781 kwgsattkat itckskktid fdsqlpsgms geklkelerg pvseppveea prqpprfith
     3841 iqsatvdese pvrfecrvep kddpnlriew yrngkmipag hryrtmydmg fvsmdilyvy
     3901 pedsgeyvck aindlgedtt raslsckklp siilqnqvpk gmkksealmq meatikkyts
     3961 evhlteddly dadkkqpprf vtqildqtel vemnsskfec qlapvgdpnm kvewffngkp
     4021 lphknrftpi ydfgyvamnf gwvypedsge ylcratnlyg mdetravirt agkpgiiyes
     4081 qlpkgmksie kiremeaawq ivpeeegeee kvrapptfvs kpepvtveeg dcsrfccrvt
     4141 ghprprvmwi inghtvvngs rykltydgmy hldipktrqy dhgkveviar ssvgesrtet
     4201 tltvkprsdd yrgvlknspr pwydygltqy qterqntele kvfderhhnv sqgieiateh
     4261 lgqkvfkepe tdwqksvksk kneeyynklm tleeeqvlke trlresshqf aipgekvvad
     4321 svakgmaqky eetleekkde ktqetvqppp rplkkphtte vdidvermkv tgkyppgpse
     4381 stvhgrevhv akqkqtqkev kgdleitrki tatetteveh kaktqervvq gptkpakppv
     4441 ftkkiqpcra feqeqarfev efdgdplptv kwyredfpiq nsadlqiytf stksvlivrq
     4501 vfmedsgvfs viaenrggka kcsanlvvee rrrqpgrggv ippsflstiq ntsvttgqla
     4561 rfdakvtgtk pldvywlkng kkitadiryk tleedntytl llletvpeds gkyecvaink
     4621 agearcdaec tvrgpqspak sakpttpgve kapavleplk dqtiregtsv afscrisgkp
     4681 vptvqwkkgd kvikpskyfq mskegdfctl riseafpede gvykciaknp agdvttsanl
     4741 rvlapdaadv lpkltplkdq ivsegqpaqf ktqvtpskpk ptiqwyrega lipqspdfqm
     4801 ihegnnavll iattyeedtg tftcrattsa gtvetsakli vkkrtgayye vpaetgpgar
     4861 tgdkpfvslg tgkdvilepi pldkgeleip lkpgdetlpw rkgrvhhrpr ladttpwrkt
     4921 kpksrdtsld klqatesqik pwteetvtlk ktpqqareip kekleevelk pakiekkeir
     4981 rpsletvdlk pftkettket kieekltrre eidkeiyeee edsrflktsr kvdetvqrkk
     5041 devkpwteek vtlkkskpek kelpketlet velkpskigk gevrkpslek vdlkpvpttv
     5101 tekvsekvte kteyleqedt slldvsrtee vkkkrkekve ekpekpeqak pwteekitlk
     5161 kskpvvkeie ketletvelk pskigkaelr kpslekvdlk pvpttvtekv tekitektey
     5221 leqedtslld vsrteevkkm rkekveekpe qakpwteeki tlkkakhlpk eieketvetv
     5281 elkpskitks eirkssvekv dlktvtketk eitqktdyre eedttllevt kdtqvkktrr
     5341 esipvekveg pkpwteekvt lkkakpekke vpkekpetve lkpsrpqkad iekpsledvd
     5401 lkpipkkvtk ektekdkyie eedtsllevt kdtetttkve kkvkrreeve kkpetkpwta
     5461 ekitlkkakv eqkeirresi eevtlkptkt ekrqvtketl ekvdlqkvtd ktqfveeedt
     5521 tlmkvdkvek veeervdkma twrrdrkpke kapyheeedk sildvkrdee stekrkevpv
     5581 pwargkkkpt ekapyheeed tslldvkkde esiekqkevp vpwargkkkp keekpelvpq
     5641 qvqpeekpee eetlektqvp wlrdkkvark iperkeepve diksvtlkpv rrerapeeke
     5701 kpeevtlkpv kripreeeek eevqlkpipk rkpeekpqee iilkpfqkpk peveeqekpk
     5761 lkpfekakpe vpeqekvqlk pfkrekpevp ekekvelkpf krekpeetlv pqkvpekpee
     5821 eqpseveevs wrrkpkkkea iipekpvekv eevkvvedkv ikkqeetied fksftlkptk
     5881 rgkkleekee keevtlkpvk rlpkeeetka eiklkpvekk veekeakplk pemaptvpee
     5941 ekptevsevp wkrgkkkpve krveevttee kkveeeekev selswrkpqk ekvkevkkeq
     6001 evedftavml kptrkekkpe ekeekeeitl kpiqkvqkee ekpeavtlkp vkvekpeave
     6061 keevpeekte lpwrrgkkpa kkivpqeevk peeigkpeer vpevkeevpk kpkvekpeee
     6121 klevklkpvk rlekpeeakp eevtlkpvkr lpkeeeekee vklkpikrek peeekpevkl
     6181 kpfkrlekpe eekpeevtlk pvkrlpkeee ekeevklkpi drkkpeeekp evklkpikrl
     6241 ekpeeekpee valkpvkrlp keeeekeeik lkpikrekpe eekpevklkp fkrlekpeee
     6301 kpeeitlkpv krrpkeeeek eevklkpikk ekpeeekpei klkpikrlek peeekpeevt
     6361 lkpvkrlpke eeekeevklk pikkekpeee kpevklkpfk rlekpeeekp eeatlkpvkr
     6421 lpkeeeekee vklkpikkek peeekpevkl kpvrrlekpe eekpeevtlk pvkrlpkeee
     6481 dkeevklkpi kkekpeeekp evklkpvkrl pkeeeekeev klkpikkekp eeekpevklk
     6541 pvkrlekpee ekpeevtlkp vkrlpkeeee keevklkpir kekpeeekpe eiklkpvkrl
     6601 dkpeeakpee vtlkpvkrlp keeeereevk lkpvkkekpe eekpeeiklk pvkrlekpee
     6661 tkpeevslkp vpkvvpekde ekpeeeapet silpwrrgkk tkkvvdfhee vtvvpidqkk
     6721 ivsevheket tiveqkveak eviekkvvve evkpevveev tevsevswrk prkpkkpvee
     6781 kevvelkpve kheeivekve ekitlkkvkk evpeveekke iqlkpvpkkp keeevpekit
     6841 lkpvkkekpe ekpkeeeipe lkpvklekpe kveeteekee vmveitrkkp kkkpeevpve
     6901 keevveteit eiteekpeei svlpwrrpkk vtekkievee ikpekvedke veevtettev
     6961 swrkqrkpkk pieeeekpek vtlkpvkrek peekpkeeip elkpiklekp ekveeteeke
     7021 evkvevtrrr pkkkpeeiqr eeievetkev vddvevekie kqeiqiedrk rkrkqrtqvd
     7081 isiidkdkki aprfiqklqp viaepestak ftctvfgspv peiswyrneq elhasekyim
     7141 tvyettasle itkvkeedag myscrasnpa gvatstvnlv ifekeeegia phfstpikpl
     7201 mveehkpall ecvvtgspvp evkwyrgeqe lkpkkgreit fnpetgeakl hilepteede
     7261 tiyrvravnk fgraecranl linnvvkvsk pevlrapkit rplpalvaer gkpltlsadf
     7321 eskptpevkw frngvelaps eknviniyen taelfipevt kkdggkyeir vqnpvgears
     7381 sgsvtvkdrp dktdevkapr fieplqpqiv aegevvimet kvesyptasf qwfhetrple
     7441 stpqvrivtq enksilmike ikseyagtyt craenvggsv tctatinlle tpweetvelv
     7501 sptfvkrlsp vrvmdgesan ltcvvqgkpt pkvewyhdsk pikegkeiti vqdtegvcsl
     7561 aitevfpeda geytcravnp vgeavcttsl iveayeyvpd seiassivat slttgqsgse
     7621 edllspketp lfdtdteesa peiikklpql iptkdgeltr levkvkgkpk pegkwykqgv
     7681 eivssqefqi eefedgtsvl tiaetypddt geivfeahnp lgvsttmtyl svegivgtke
     7741 yrkpewvthm eemqealrat qtvprfiqei tdvyvkegdt avfecsysgn ptpdvvwykn
     7801 dkmlmstqnl kvriydddkk tvltikhate eddgayvcki tsetglatsk aklhvtdvtd
     7861 ekkfyeeeee veeqtievkp kekpqkkklr kakevkekvk tervkvdkke vrkeklvpke
     7921 qpeekkivdi eetqilestd iieekptevs qakkivpiqe pvlteatasl kkiddekpkd
     7981 mvpkpeerak rvieeregiv vvsevttedm apdftveskv draqitsesl qtvtvsevht
     8041 etsvqevrra eakqrkakkt vvteeeifee rpkevvveek pkkkkktekv kvreevtiee
     8101 iverqrenia reveeimeml hakefgpgea plrelatigy lvrqgvsvne ineslyrten
     8161 fpalktpeaq nalvqlvere ghgplitqvl teetttdesv vaatvgfraf mrmielqhvt
     8221 veevlthfap eafrprawev teatevetee rvtervdvvr ktevhfmerr derkavrtqd
     8281 irdikeyedt rqthttlrar kdrkpreetk edrdegvqvv eieeiedteq ekmkkkrkdv
     8341 eeieeeietv ivetdskgrl aqkkkkrddl eekedveeee eiiekvrkel kpkrpkigrd
     8401 eveleeieef dvtedksmps imvnvpsqrh qvvpldrtte qapgkpelek akltvdtita
     8461 ltehivpvqe keisdisrik pierkaslsi spvepystte ttvqastgef adtfkptsye
     8521 atpgiipseg vqvsetlvsd agvtslsvsk qeigrtaevs vtlqeattvy etmvrekevp
     8581 tedfvtpqtv kaedtilpql slsvyevheg ltedkleplk tvatkprvnv taveplvvee
     8641 vraedkpgky ypelivptev atqsvisqrq rvteemhape kegeyipgrl ppsqvaqigi
     8701 sygnetavvq qnvvqesegv fisekktetf eattnvtvle gvsvstvqtq dtetsltiee
     8761 tkkavadlnv iemtpavtve tvpsekekey qpgdkpdtkt aetsisslei gsiqstvvqe
     8821 segvyrpdek ptkalaetsi rpeehvmise iqtadypsdf reelkyvqes gtvsvqltea
     8881 kmiqetlthd reermeemvk peermvdtsy dvnrsvevfq ttsvekeadl rvfempeshr
     8941 gktvpthpvv slevemtqpe dnlgevkkev pssgtakveh vslqetivge tvvaenlapv
     9001 ekdkapetkv aeiamneves lhttmvvvse keteytqvsd vkgayastef ttqvapmlee
     9061 vrshspteey lpdqkptsgi aqpshvples itvavqelae kedlyktdvr pdqktanval
     9121 teltsisipe vmqvitassa etlkketkmk eekgrvevee lsstiisevi snetedvftt
     9181 katpkdqkad fnisgreaae ttqvtamtea edlavkrpee qkgkpqvdel tpltvshvet
     9241 geaekelpta evpkektaqp nifgrdvaet mqvltvtsae elaapkapeg qkgkpqveel
     9301 tplsvsqvvs qeaesalpap eapterkaqp dmtgrdiaet mevltmtsad ilaedvkpte
     9361 qkgmpgleef vplsvsqtvs tekedvlpsp eapttktaqp sltgrdiaet mqvvmmtsek
     9421 elitalapet hkgipnveel tpltvsqtvs heaedvfekt aapsevtakp afsgrevaqt
     9481 semmivtnvg elekqvlpde qkgqaqldel mpltvsqvis geveeglpea ekpkektaqp
     9541 gilgrdiaet tqvltvtsae elaapkapeg qkgkpqveel apltvsqvvs eeaegilpap
     9601 eapterkaqp dmtgrdiaqt mevltmtsvd tftedkkpde qkghpeleef vplsvtqtvs
     9661 nemedvlpsp eapstkiaqp sltgrdvaet mqiltmtsee elitaqtpeq hkgkpnveel
     9721 tpltvsqtvs qeaeeileka aaptevtakp afsgrevaqt seimivtnvg elekqvlpde
     9781 qkgqpqldel tpltisqiis geveeglpea ekpkektaqp gilgrdiaet tqvltvtsae
     9841 eleapkapeg qkgkpqveel apltvsqvvs qeaegilpvp eapterkaqa dltgrdvaem
     9901 mevltmasvd tltedkkpae qkgvpgleef vplsvsqtvs tekeevlpsp eapttkvaea
     9961 sltgrdiaet mqvvtmtsee elisarvpeq hkgifnveel tsltvsqtis heteeileka
    10021 aalgevtakp aftgrevaqt setlivtnvg elekqilpee qkgkprvdem tsitvsqvvs
    10081 geveerlpea ekpkektaqp smlgrdiaet tqvltmtsae elaapkapee qkgkpqveel
    10141 apltvsqvvs qeaegvlpvp eapterqaqp dltgrdvaqi tvvmsmasad vmiddkkpee
    10201 qkgvpgveef vplsvsqtvs tekedvlpsp eapttkmaqp sltgrdiaet mqvvtmtsee
    10261 elitalapet hkgkpnveel tsltvsqtis qetevileks aapseatakp aftgreiaqt
    10321 seilavlnvg elekhvlpee qkgkpkleel tpltvsqiis geaeeglpta evpkektaep
    10381 smlgrdiaet tqvltmasae elmapkapee qkgkpqveel apltisqvvs qeaegvlpvp
    10441 eapterkaqp dltgrdvaqt tvvmsmanvd imieekkpee qrgipgleef vplsvsqtvs
    10501 tekedvlpsp evpttkmaqp sltghdvaet lqvltmvsee qlttkapeeq kgkpnveelt
    10561 sltvsqtvsh eaedilekav apseatakpa flgrevaets qvltvttvge lqkdvlpeeq
    10621 kgkpqleelm pltvsqivsg eaeerlpeae kpkektaeps mlgrdiaett qvltmasaee
    10681 llapkvpeeq kgkpqveela pltvsqvvsq eaegtlpipe aptertaqpd ltgrdvaetm
    10741 evltmasldt liedkkpaaq tgapgleefi plsvsqmvst ekedvlpspe vptekmaqps
    10801 ligrdvaetm qvltmisame lttghapdqq kgvpnveelt sltvsqtvsh eteeilekav
    10861 apstvtakaa lsgrevaets qvltvsnvee feksilpegq kgkprldelt sitvsqvlsn
    10921 eleetlpspe kpsekiaqpq migrevaeqt evltvtnvee lkklekpegq tgkpdveems
    10981 fitvsevfst emekempspe vpkertalpq lssievaett evltmisaee llkskapeeq
    11041 kvqpkldelt pltvshvvse eaeealptae ipkektaqps llgrdvaets qvltmasaee
    11101 llaakvpeeq kgkpqieela pltvsqvvsq eaeealpsae vpvereaqps mtsrdvaqtm
    11161 lvtsmsnvdi maeskipeeq kgvpgleefv slsvsqtvst ekedvlpspe vptekmaqps
    11221 ligrdvaett qiltmisate lttdrapeqq kgvpnveelt sltvsqavsh eteeilekle
    11281 apgtvtakaa lsgrevaets qvltvsnvee feksvlpeeq kgkprldelt sitvsqvvsn
    11341 eleetlpspe kpsekvaqpq migrevaekt evltvsnvee mqklekpegq kgipdveems
    11401 fitvsevvst eaekelpape apkerkalpq lsgievaeks evltmtmtee fakpkapeeq
    11461 kgkpqleell slsvsevalg evekglptpd kpteetakps llgiqvaeks qviassitge
    11521 lqesikpdtk qiipeqipfe sieqsqtltq erestfvvek tttsataevs frvseslevt
    11581 qvtatekesk evikgvakea saqadvierk valktevqpe dmasefkvkk pegktakgvd
    11641 eiqqgvivte hltahefese lpesvlpfak larvsiqadh lehivettea paqqhhitit
    11701 qhttkhdlpq qqsiesdtev ieeyttkfrr gstedataav ktkktivrkk pkttgeediv
    11761 vvieeelddi kpkipsipkk qfmpieevtt vsdveeivsa rideaeeivp keeekvevti
    11821 tevqdtdkkk kvtkkqfvrr rgktrqvtee itveeegkap ettvvtkpve iieedieerp
    11881 kiekeeyvve evpeektipl kprkkltptv qqveeqvtvt eeitkegkpr kttkkkvitr
    11941 kgkeqqvtev vtveeqgkap itsvtegpve evveeiiqkl papkyakpse veevreqvtv
    12001 teevtrlgkp kkttkkkiik rkgkeqqvte vvtveeegka ptttvtegpv eevvieeike
    12061 lpipefvkpt eveevreqvt vteeltkmgk pkktikkkii krkgkeqqvt evvtveeqgk
    12121 apvttvkegp veevveetit pllapeyakp seveevreqv tvtqevtklg kpkkttkkki
    12181 ikrkgkeqqv teivtveeeg kapkttvteg pveeiieeei kpefikprev eevreqvtvt
    12241 qevtregkpk ktikkkiikk kgkeqqvtev vtveeqgkap vttvtegpve evvdetvkal
    12301 papeyakpse veevreqvtv tqevtklgkp kkttkkkiik rkgkeqqvte ivtveeegka
    12361 pkttvtegpv eeiieeeikp efikprevee vreqvtvtqe vtregkpkkt ikkkiikkkg
    12421 keeqvtevvt veeqgkapvt tvnegpveev vdetvkalpa peyaepseve evreqvtvtq
    12481 evtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk ttvtegpvee iieeeikpef
    12541 ikpreveevr eqvtvtqevt regkpkktik kkiikkkgke eqvtevvtve eqgkapvttv
    12601 negpveevvd etvkalpape yaepseveev reqvtvtqev tklgkpkktt kkkiikrkgk
    12661 eqqvteivtv eeegkapktt vtegpveeii eeeikpefik preveevreq vtvtqevtre
    12721 gkpkktikkk iikkkgkeqq vtelvtveeq gkapvttvte gpveevvdet vkalpapeya
    12781 kpseveevre qvtvtqevtk lgkpkkttkk kiikrkgkeq qvteivtvee egkapkttvt
    12841 egpveeiiee eikpefikpr eveevreqvt vtqevtregk pkktikkkii kkkgkeqqvt
    12901 evvtveeqgk apvttvtegp veevvdetvk alpapeyakp seveevreqv tvtqevtklg
    12961 kpkkttkkki ikrkgkeqqv teivtveeeg kapkttvmeg pveevvieei qelpipefvk
    13021 pteveevreq vtvteevtrl gkpkkttkkk iikrkgkeqq vteivtveeq gkapvttvte
    13081 gpveevvdet ikslpapeya kpseveevre qvtvtqevtk lgkpkkttkk kivkrkgkeq
    13141 qvteivtvee egkapkttvt egpveevvee evkpllapef vkpseveevh eqvtvtqevt
    13201 regkpmktik kkiikkkgka qqvtevvtve eegkvpvttv kegpveevvd etvkalpapk
    13261 yakpseveev reqvtvtqei tklgkpkktt krkvikrkgk eqqvtevvtv eeegkapett
    13321 vtegpveevl eeeikplpgp efvkpsevee vreqvtvtee vtregkpkkt ikkkiikkkg
    13381 keqqvteivt veeqgkapvt svtegpveev vdetikqlpa pkyakpseve evreqvtvtq
    13441 emtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk ttmtegpvee vveeeikplp
    13501 alefvkpsev eevreqvtvt eevtregkpk ktikkkiikk kgkeqqitev vtveeqgkap
    13561 vttvtkapve eivdetikel palefakpse veevreqvtv tkevtkegkp kktikrkvik
    13621 kkgkeqqvte ivtveeegka pkttitegpv eevveeeikp lpalefvkps eveevreqvt
    13681 vteevtregk pkktikkkii kkkgkeqqvt evvtveeqgk apvttvqegp veevidetik
    13741 slpapeyakp seveeireqv tvteeltklg kpkkttkkki tkrkgpeqqi teivtveeeg
    13801 kapvttvteg pveevvkevl kplpvpkvvk pseveeireq vtvteemtke gkpkkiikkk
    13861 vikrkgreqt vqevvtveek gkapvttvtv gpteeiveet vktlpapeya kpseveevre
    13921 qvtvtqevtk egkpkkttkk kvikrkgkeq qvtevvtvee qgkapvttvt egpveevvee
    13981 llkpliapey vkpseveevr eqvtvteelt kegkpkkvik kkvikrrgre qqvkevvtve
    14041 eqgkapvttv tegpteeive etikalpape dakpseveev reqvtvtqev tkegkpkkti
    14101 kkkvtkrkgk eqqvtevvtv eekgkapvtt vtegpveeiv eetvkalpap elvkptevte
    14161 vpeeltvtev vtkegktkki vkkkvpkpkk egieapeeap eevpveetkk iiklqrlkvs
    14221 kfestkldiq etslgkpqfa qiklrktpva krpskkdekf prillrsrii aiewppspkn
    14281 lkvdtfepie lqngilsrnv deaaalpkpk kkkikrieke iaklekldqe feelkkelpl
    14341 ekleepilee kperkvkkkk kpedkakegv kpqlmrisik eqkpikpkia epatplfaqi
    14401 klkkapakpk kkpeeevskf pkillrsrit ahewppsvky paisvlepny iqngilsrtm
    14461 eealklkkvk hkkmklpeke ltelekldqe feelkkvple kveeitpyer kpkpekpeee
    14521 kpkklvigkg krrpaeeedl etvqlkkipe kipsapeeae kvpkkkdevd kpkkekeeke
    14581 ypelkpfepy dieapevele erekpeypep ekpkekkvpg kkpkkerktp aqeteavpiv
    14641 pgvpkpkepe eeeeikrrip erpppteepe kiklkpwkkg ekpeeeekkp edyikfvpkd
    14701 ddivetvevt ttveetpdkk qrkikkkkti tkpkdekpki ieettieeeg fepvvtveef
    14761 dvdkyeavpv esvekaapve eikeeivtse iiteegtkkt vrkkrvtkkr egkervteev
    14821 tveeegkepi tatielhedt iseiheqakh krptkpkade klaedvtekt veqqeiieev
    14881 kevkptkkvi kkekpvkakd evteevveei iieepekqea kpiveeeetv iitehdvrka
    14941 pkkkkevvik eeeeifetie tpthkiirkk irpmkkgeke vvveeiiqkp reetvemeeq
    15001 qivevvetpt kkivkkkkev vkddgvpeel veeiiiekpq kkkakptvee ekkkvkvtkv
    15061 dtkkapekpe evveeeeeii etietptkki irkkpkdkkd kiieeviqkt ieeakameeq
    15121 eivevietpt kkvikkkttt ieegkapeek veeiviekpk evieeeeeii etietptkri
    15181 vrkkpkdkkd kiveeviqkt ieeaktmkeq eiievietpt ktvikkkkat ikegkapeee
    15241 veeiviekpi kekakkkilp kegveitevi tetsveelie eelkpeeakp seeveeekvk
    15301 itevaikkvp kkkkpskave aeekeevvee ikpekpkeek akptvtekeg ievtevisev
    15361 apeklpeeak pkkkkateik eteekvitkk vkvkeapkea vpgeeeqpae evdelvpekp
    15421 kktkakkevt pqdsivttev vpitkeeele vekpeeekat vveekeekak veekkvtvkk
    15481 apkkklkpte aeeeqveevv eeitpeepkk rkakkavipk eeiqttevip gitaeeipee
    15541 ekpeeekavp keeekveeea kvtevsvkkk erkkkekpvt ngevieekpk vkeipeekpk
    15601 eevpqvpeek ekpeeekpke vkpkekkpke ekpkkpieek peevkpeeek pevkpeeekp
    15661 keekpkkkkp aekkpkisee erpedkveei pekkpeevkt reeiipkdtv qttevipetv
    15721 aeeiptkkpk erkavpveek eekveitevp vekapekkee kvpeekptea apekpkeakv
    15781 mkkkkkkpak kdeieewvep eyerpvlepm pekivwepkk kkekkplpes lqklvpqkie
    15841 rkemkptklk ysepaetvqf asiklkkvav pkkkeadetk fpkimlrsrm tfvgdyppel
    15901 qipkitylee npvqtgilsr nteealkvlk rkvkkvklse kevtqlekld lefeelkkvp
    15961 lekiedksvy erqpkkpaek peepkkvpig kgkvpeeekv epekvklkkv pekkpeevee
    16021 plkkpkkpep eeevkpekkk kpkvklehde lkpldfdrpe lekyepeeye kpeepapepv
    16081 pkpyekepkk kpdqeveqip lkkgkpkspe pdeepevkfr iptkekpeee peqitlkpwk
    16141 kekpeeekek eipkkeeekv pkppkeeped vtvqrkaktk kpkekpkeek eeevtvkkpe
    16201 eekpeevaee vtlkkepekp veeapaevti kkpvveekev eeeetitevi lkkkpekpee
    16261 vveeavvkkp kkkkpvkeee vveevtvtkv vteeekeeek vvikkpkkks ipeiteevte
    16321 eitlkkpeek apeevteeik kpkkkkpvke evaeeitikk pvveekkeee vpeevtlkpk
    16381 kpeekpviee vteeitlkkp vpeevteeva ikkpkkkkpv eeeaadeitl kkpvvvekee
    16441 eeiteevtlq kkkkpekkpv peeiseeitl kkpvpkeeek apeevteeia ikkpkkkkpv
    16501 keeaadevti kkpvveekke eetpeevtlk prkpkekpvp eevkeevtlk kpvpeekekv
    16561 peevteeiai kkpkkkkpve eeaadevtik kpvvvekeee eiteeitlgk kkkpekkpvp
    16621 eeiteeitlk kpvpkeeeka peevtekiev kkpkkkkpve eeaadevtik kpvvvekeee
    16681 eapaeitlkr rpkekpveei teevteeiti kkptpeekek apeevsekve vkkpkkkkpv
    16741 veeaadevti kkvvveekke eeapeevtlk prpkkksvte eevaevtltk pkpieeekkp
    16801 eeseltikkk pkkkpvieee aaevtikkpv pvepdeapee itirkkkkpe kkpkvteeve
    16861 etavtikkar apekpteeee ittdvqikkk kpqkkveeva adeltikkie eveqpeeeee
    16921 vqeftfkrkp pklppkpiee iyedvtlrkl rpkrksrpdi keitetenvt frprttktke
    16981 dveqefkisl ntyeeedism sgkvrlkpkk kpltyseetg eetiriiqev eddsgpiiee
    17041 iiesdeegkd eysieeldtd emrlplrrkk kkvpkpykve dieegdvklk lkrerkysie
    17101 eadeslalkl kskrrvstyd eeeatlsitr eediseevei eyvvrdgdtm fsicsyvaet
    17161 deainlvege rvyvidhtnq dwwfvkkhlt eekgwvpaqy llnevhytly lqrklhekid
    17221 klpvfekpap gdkssapkfv eklqpihtpd gytiqfecqv egvprpqitw frqtaiikps
    17281 pdfqmyyddd nvatliirev fpedagtftc vaknaagfas stteliveap lsdhgsdltg
    17341 psrkslsres sladilegip ptfsrkpkak yvdegedvil ecrlvavpep eitwyykdtq
    17401 ittkeniiia tesdmhmycs vikitkvqkk qegkytivar nregeatiei pmkvktgkse
    17461 ppeileplqs yvvregetvv lstqivgnpa pkvtwfkngk plkgltpkqd ghvntltliq
    17521 pqvsdtgeys vvatndlgta etkatltvei nlkpslainf qqhtfehfsv tkeipsgape
    17581 pplfterfqe ltvpekgtfk lvakvtgnpv pevtwlrnnk plekspnite tydgenivle
    17641 irnansevdt gdykcvasnp vgkashgarv tvdvdkvtft ktlekevvvd eyktleltce
    17701 tshtvstkww hndkeisgmd hreiieegrv hklvikktnp tdegtykctv knqstssnvt
    17761 vkatkpefvk klqdcevker dvaileveit sqtvdvkwyk dgeqlgpgke klefvkegtv
    17821 rkllirstsv hdegeytcsl ldqectaevt vielppeiit kmqdvtiarg eratfdielt
    17881 kgdalvrwfk dgqelqfseh vrlsidgkrq klkiydtese dagvyscevg qqkssakltv
    17941 eepavefitk lpdvtlvpln sdavfviels qdvpvtwmrk tqvikespky tiidegkvkk
    18001 livkkctted iseytamvan vktssrlrve vietppkisp dtprkykvrk dedvdivvkf
    18061 sgtpkpedew tvngrvvtks kraiptidee savltirkiq eedvgdytlk lvnsvgetsi
    18121 einvvivqvp sapgapeple ttndsvtlhw kkpdsdgnsp iveyilehqe ktettwtkit
    18181 etitetthkv tklttnkeyt frvtavneag pgetsptspy vkiskpstae ppvileplks
    18241 iviglgetvt lscviggtpa peitwlrnde ifedtsitye nhitkytire ttetssakft
    18301 vkaknnvgsa ettcelkiqe spkiiydenl tnqklpmngq wkieiqitgf pkpevtwtkn
    18361 gkevdekrvs ihreertstv sisslvredt atymakaenq agsssvdlhl kvidkpskpq
    18421 gpvvfkeirq drvtlewrpp addggielek ytiekcepgk twvkvvdidk evesfcvhkl
    18481 qqnaeykfri iarnavgase plesdtvkmr tsfeppgapr gplevsgmtk tsftikwqpp
    18541 endggtpitd yiieikeesk ktwqkigstk hetthfsvsd lktdvpynfr itaknsvgsg
    18601 ppyvaeepia pgrrikitkl tessmyallg ifpptkivis ktppsspqhv hvtnitsksa
    18661 tlswsppast ggteltgyvi ekrpligkga rwtkvvtlda ttlqycvenl keseflfrif
    18721 aennqglsvp ansepvtlkt hasvpsppta pleirqiaan tvviswgrpd sdggaplegy
    18781 kvairdtkrt mwmevarvnt eiqklnirdl qenhmyliri farneiglsd pleseepfki
    18841 ipaseltvve piaeatekge tasisfsten tsswlrehnm dadihsyara rllrqdeyff
    18901 riwhyakklf e
//
LOCUS       XP_034182949           17489 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X20 [Osmia lignaria lignaria].
ACCESSION   XP_034182949
VERSION     XP_034182949.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327058.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182949.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..17489
                     /product="sallimus isoform X20"
                     /calculated_mol_wt=1989515
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1318..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7689..7693
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7756..7759
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8230..8234
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8267..8271
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8297..8300
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          15727..15779
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(15732,15734,15740..15741,15758..15760,15773,15775,
                     15777..15778)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          15815..15905
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          15832..15836
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          15845..15849
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          15871..15875
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          15885..15890
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          15898..15901
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          15939..16032
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          15956..15960
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          15969..15973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          15994..16002
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16012..16017
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16025..16028
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16040..16126
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16057..16061
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16070..16074
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16092..16096
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16106..16111
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16119..16122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16160..16250
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16177..16181
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16190..16194
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16215..16219
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16230..16235
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16243..16246
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16263..16340
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          16273..16277
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16306..16313
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16323..16328
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16332..16335
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16344..16429
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16361..16365
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16373..16377
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16399..16403
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16413..16418
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16422..16425
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16434..16518
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16451..16455
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16463..16467
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16488..16492
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16502..16507
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16511..16514
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16624..16704
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16632..16636
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16645..16649
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16670..16674
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16684..16689
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16697..16700
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16708..16799
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(16708,16773,16788)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(16789..16790,16792..16793)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          16810..16896
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16827..16831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16840..16844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16862..16866
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16876..16881
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16889..16892
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16920..16990
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16920..16923
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16932..16936
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16956..16960
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16970..16975
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16983..16986
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16994..17086
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(16994,17060,17075)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <17064..17379
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(17076..17077,17079..17080)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..17489
                     /gene="sls"
                     /coded_by="XM_034327058.2:177..52646"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikdvtektve qqeiieevke vkptkkvikk ekpvkakdev teevveeiii eepekqeakp
    13501 iveeeetvii tehdvrkapk kkkevvikee eeifetietp thkiirkkir pmkkgekevv
    13561 veeiiqkpre etvemeeqqi vevvetptkk ivkkkkevvk ddgvpeelve eiiiekpqkk
    13621 kakptveeek kkvkvtkvdt kkapekpeev veeeeeiiet ietptkkiir kkpkdkkdki
    13681 ieeviqktie eakameeqei vevietptkk vikkktttie egkapeekve eiviekpkev
    13741 ieeeeeiiet ietptkrivr kkpkdkkdki veeviqktie eaktmkeqei ievietptkt
    13801 vikkkkatik egkapeeeve eiviekpike kakkkilpke gveitevite tsveelieee
    13861 lkpeeakpse eveeekvkit evaikkvpkk kkpskaveae ekeevveeik pekpkeekak
    13921 ptvtekegie vtevisevap eklpeeakpk kkkateiket eekvitkkvk vkeapkeavp
    13981 geeeqpaeev delvpekpkk tkakkevtpq dsivttevvp itkeeeleve kpeeekatvv
    14041 eekeekakve ekkvtvkkap kkklkpteae eeqveevvee itpeepkkrk akkavipkee
    14101 iqttevipgi taeeipeeek peeekavpke eekveeeakv tevsvkkker kkkekpvtng
    14161 evieekpkvk eipeekpkee vpqvpeekek peeekpkevk pkekkpkeek pkkpieekpe
    14221 evkpeeekpe vkpeeekpke ekpkkkkpae kkpkiseeer pedkveeipe kkpeevktre
    14281 eiipkdtvqt tevipetvae eiptkkpker kavpveekee kveitevpve kapekkeekv
    14341 peekpteaap ekpkeakvmk kkkkkpakkd eieewvepey erpvlepmpe kivwepkkkk
    14401 ekkplpeslq klvpqkierk emkptklkys epaetvqfas iklkkvavpk kkeadetkfp
    14461 kimlrsrmtf vgdyppelqi pkityleenp vqtgilsrnt eealkvlkrk vkkvklseke
    14521 vtqlekldle feelkkvple kiedksvyer qpkkpaekpe epkkvpigkg kvpeeekvep
    14581 ekvklkkvpe kkpeeveepl kkpkkpepee evkpekkkkp kvklehdelk pldfdrpele
    14641 kyepeeyekp eepapepvpk pyekepkkkp dqeveqiplk kgkpkspepd eepevkfrip
    14701 tkekpeeepe qitlkpwkke kpeeekekei pkkeeekvpk ppkeepedvt vqrkaktkkp
    14761 kekpkeekee evtvkkpeee kpeevaeevt lkkepekpve eapaevtikk pvveekevee
    14821 eetitevilk kkpekpeevv eeavvkkpkk kkpvkeeevv eevtvtkvvt eeekeeekvv
    14881 ikkpkkksip eiteevteei tlkkpeekap eevteeikkp kkkkpvkeev aeeitikkpv
    14941 veekkeeevp eevtlkpkkp eekpvieevt eeitlkkpvp eevteevaik kpkkkkpvee
    15001 eaadeitlkk pvvvekeeee iteevtlqkk kkpekkpvpe eiseeitlkk pvpkeeekap
    15061 eevteeiaik kpkkkkpvke eaadevtikk pvveekkeee tpeevtlkpr kpkekpvpee
    15121 vkeevtlkkp vpeekekvpe evteeiaikk pkkkkpveee aadevtikkp vvvekeeeei
    15181 teeitlgkkk kpekkpvpee iteeitlkkp vpkeeekape evtekievkk pkkkkpveee
    15241 aadevtikkp vvvekeeeea paeitlkrrp kekpveeite evteeitikk ptpeekekap
    15301 eevsekvevk kpkkkkpvve eaadevtikk vvveekkeee apeevtlkpr pkkksvteee
    15361 vaevtltkpk pieeekkpee seltikkkpk kkpvieeeaa evtikkpvpv epdeapeeit
    15421 irkkkkpekk pkvteeveet avtikkarap ekpteeeeit tdvqikkkkp qkkveevaad
    15481 eltikkieev eqpeeeeevq eftfkrkppk lppkpieeiy edvtlrklrp krksrpdike
    15541 itetenvtfr prttktkedv eqefkislnt yeeedismsg kvrlkpkkkp ltyseetgee
    15601 tiriiqeved dsgpiieeii esdeegkdey sieeldtdem rlplrrkkkk vpkpykvedi
    15661 eegdvklklk rerkysieea deslalklks krrvstydee eatlsitree diseeveiey
    15721 vvrdgdtmfs icsyvaetde ainlvegerv yvidhtnqdw wfvkkhltee kgwvpaqyll
    15781 nevhytlylq rklhekidkl pvfekpapgd kssapkfvek lqpihtpdgy tiqfecqveg
    15841 vprpqitwfr qtaiikpspd fqmyydddnv atliirevfp edagtftcva knaagfasst
    15901 teliveapls dhgsdltgps rkslsressl adilegippt fsrkpkakyv degedvilec
    15961 rlvavpepei twyykdtqit tkeniiiate sdmhmycsvi kitkvqkkqe gkytivarnr
    16021 egeatieipm kvktgksepp eileplqsyv vregetvvls tqivgnpapk vtwfkngkpl
    16081 kgltpkqdgh vntltliqpq vsdtgeysvv atndlgtaet katltveinl kpslainfqq
    16141 htfehfsvtk eipsgapepp lfterfqelt vpekgtfklv akvtgnpvpe vtwlrnnkpl
    16201 ekspnitety dgenivleir nansevdtgd ykcvasnpvg kashgarvtv dvdkvtftkt
    16261 lekevvvdey ktleltcets htvstkwwhn dkeisgmdhr eiieegrvhk lvikktnptd
    16321 egtykctvkn qstssnvtvk atkpefvkkl qdcevkerdv aileveitsq tvdvkwykdg
    16381 eqlgpgkekl efvkegtvrk llirstsvhd egeytcslld qectaevtvi elppeiitkm
    16441 qdvtiarger atfdieltkg dalvrwfkdg qelqfsehvr lsidgkrqkl kiydteseda
    16501 gvyscevgqq kssakltvee pavefitklp dvtlvplnsd avfvielsqd vpvtwmrktq
    16561 vikespkyti idegkvkkli vkkcttedis eytamvanvk tssrlrvevi etppkispdt
    16621 prkykvrkde dvdivvkfsg tpkpedewtv ngrvvtkskr aiptideesa vltirkiqee
    16681 dvgdytlklv nsvgetsiei nvvivqvpsa pgapeplett ndsvtlhwkk pdsdgnspiv
    16741 eyilehqekt ettwtkitet itetthkvtk lttnkeytfr vtavneagpg etsptspyvk
    16801 iskpstaepp vileplksiv iglgetvtls cviggtpape itwlrndeif edtsityenh
    16861 itkytirett etssakftvk aknnvgsaet tcelkiqesp kiiydenltn qklpmngqwk
    16921 ieiqitgfpk pevtwtkngk evdekrvsih reertstvsi sslvredtat ymakaenqag
    16981 sssvdlhlkv idkpskpqgp vvfkeirqdr vtlewrppad dggielekyt iekcepgktw
    17041 vkvvdidkev esfcvhklqq naeykfriia rnavgasepl esdtvkmrts feppgaprgp
    17101 levsgmtkts ftikwqppen dggtpitdyi ieikeeskkt wqkigstkhe tthfsvsdlk
    17161 tdvpynfrit aknsvgsgpp yvaeepiapg rrikitklte ssmyallgif pptkiviskt
    17221 ppsspqhvhv tnitsksatl swsppastgg teltgyviek rpligkgarw tkvvtldatt
    17281 lqycvenlke seflfrifae nnqglsvpan sepvtlktha svpspptapl eirqiaantv
    17341 viswgrpdsd ggaplegykv airdtkrtmw mevarvntei qklnirdlqe nhmylirifa
    17401 rneiglsdpl eseepfkiip aseltvvepi aeatekgeta sisfstents swlrehnmda
    17461 dihsyararl lrqdeyffri whyakklfe
//
LOCUS       XP_034182959           17488 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X21 [Osmia lignaria lignaria].
ACCESSION   XP_034182959
VERSION     XP_034182959.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327068.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182959.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..17488
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..17488
                     /product="sallimus isoform X21"
                     /calculated_mol_wt=1989387
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1318..2114
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6768..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6797..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7689..7693
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7756..7759
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8230..8234
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8267..8271
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8297..8300
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <10115..10768
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <10821..10974
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          12249..12338
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12266..12270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12279..12283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12304..12308
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12318..12323
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12348..12439
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12365..12369
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12378..12382
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12405..12409
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12419..12424
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12432..12435
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12454..12543
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12471..12475
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12484..12488
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12509..12513
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12523..12528
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12538..12541
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12556..12642
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12573..12577
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12586..12590
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12611..12615
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12625..12630
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12638..12641
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12659..12749
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12676..12680
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12689..12693
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12715..12719
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12729..12734
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12742..12745
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          12797..12883
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          12816..12820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12829..12833
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12855..12859
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12869..12874
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          12921..13012
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          12938..12942
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          12951..12955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          12978..12982
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          12992..12997
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          13005..13008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          15726..15778
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(15731,15733,15739..15740,15757..15759,15772,15774,
                     15776..15777)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          15814..15904
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          15831..15835
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          15844..15848
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          15870..15874
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          15884..15889
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          15897..15900
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          15938..16031
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          15955..15959
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          15968..15972
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          15993..16001
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16011..16016
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16024..16027
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16039..16125
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16056..16060
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16069..16073
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16091..16095
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16105..16110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16118..16121
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16159..16249
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16176..16180
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16189..16193
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16214..16218
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16229..16234
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16242..16245
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16262..16339
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          16272..16276
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16305..16312
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16322..16327
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16331..16334
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16343..16428
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16360..16364
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16372..16376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16398..16402
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16412..16417
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16421..16424
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16433..16517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16450..16454
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16462..16466
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16487..16491
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16501..16506
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16510..16513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16623..16703
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16631..16635
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16644..16648
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16669..16673
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16683..16688
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16696..16699
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16707..16798
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(16707,16772,16787)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(16788..16789,16791..16792)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          16809..16895
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          16826..16830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16839..16843
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16861..16865
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16875..16880
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16888..16891
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16919..16989
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          16919..16922
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          16931..16935
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          16955..16959
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          16969..16974
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          16982..16985
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          16993..17085
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(16993,17059,17074)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <17063..17378
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(17075..17076,17078..17079)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..17488
                     /gene="sls"
                     /coded_by="XM_034327068.2:177..52643"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk rtgayyevpa etgpgartgd
    10021 kpfvslgtgk dvilepipld kgeleiplkp gdetlpwrkg rvhhrprlad ttpwrktkpk
    10081 srdtsldklq atesqikpwt eetvtlkktp qqareipkek leevelkpak iekkeirrps
    10141 letvdlkpft kettketkie ekltrreeid keiyeeeeds rflktsrkvd etvqrkkdev
    10201 kpwteekvtl kkskpekkel pketletvel kpskigkgev rkpslekvdl kpvpttvtek
    10261 vsekvtekte yleqedtsll dvsrteevkk krkekveekp ekpeqakpwt eekitlkksk
    10321 pvvkeieket letvelkpsk igkaelrkps lekvdlkpvp ttvtekvtek itekteyleq
    10381 edtslldvsr teevkkmrke kveekpeqak pwteekitlk kakhlpkeie ketvetvelk
    10441 pskitkseir kssvekvdlk tvtketkeit qktdyreeed ttllevtkdt qvkktrresi
    10501 pvekvegpkp wteekvtlkk akpekkevpk ekpetvelkp srpqkadiek psledvdlkp
    10561 ipkkvtkekt ekdkyieeed tsllevtkdt etttkvekkv krreevekkp etkpwtaeki
    10621 tlkkakveqk eirresieev tlkptktekr qvtketlekv dlqkvtdktq fveeedttlm
    10681 kvdkvekvee ervdkmatwr rdrkpkekap yheeedksil dvkrdeeste krkevpvpwa
    10741 rgkkkpteka pyheeedtsl ldvkkdeesi ekqkevpvpw argkkkpkee kpelvpqqvq
    10801 peekpeeeet lektqvpwlr dkkvarkipe rkeepvedik svtlkpvrre rapeekekpe
    10861 evtlkpvkri preeeekeev qlkpipkrkp eekpqeeiil kpfqkpkpev eeqekpklkp
    10921 fekakpevpe qekvqlkpfk rekpevpeke kvelkpfkre kpeetlvpqk vpekpeeeqp
    10981 seveevswrr kpkkkeaiip ekpvekveev kvvedkvikk qeetiedfks ftlkptkrgk
    11041 kleekeekee vtlkpvkrlp keeetkaeik lkpvekkvee keakplkpem aptvpeeekp
    11101 tevsevpwkr gkkkpvekrv eevtteekkv eeeekevsel swrkpqkekv kevkkeqeve
    11161 dftavmlkpt rkekkpeeke ekeeitlkpi qkvqkeeekp eavtlkpvkv ekpeavekee
    11221 vpeektelpw rrgkkpakki vpqeevkpee igkpeervpe vkeevpkkpk vekpeeekle
    11281 vklkpvkrle kpeeakpeev tlkpvkrlpk eeeekeevkl kpikrekpee ekpevklkpf
    11341 krlekpeeek peevtlkpvk rlpkeeeeke evklkpidrk kpeeekpevk lkpikrlekp
    11401 eeekpeeval kpvkrlpkee eekeeiklkp ikrekpeeek pevklkpfkr lekpeeekpe
    11461 eitlkpvkrr pkeeeekeev klkpikkekp eeekpeiklk pikrlekpee ekpeevtlkp
    11521 vkrlpkeeee keevklkpik kekpeeekpe vklkpfkrle kpeeekpeea tlkpvkrlpk
    11581 eeeekeevkl kpikkekpee ekpevklkpv rrlekpeeek peevtlkpvk rlpkeeedke
    11641 evklkpikke kpeeekpevk lkpvkrlpke eeekeevklk pikkekpeee kpevklkpvk
    11701 rlekpeeekp eevtlkpvkr lpkeeeekee vklkpirkek peeekpeeik lkpvkrldkp
    11761 eeakpeevtl kpvkrlpkee eereevklkp vkkekpeeek peeiklkpvk rlekpeetkp
    11821 eevslkpvpk vvpekdeekp eeeapetsil pwrrgkktkk vvdfheevtv vpidqkkivs
    11881 evhekettiv eqkveakevi ekkvvveevk pevveevtev sevswrkprk pkkpveekev
    11941 velkpvekhe eivekveeki tlkkvkkevp eveekkeiql kpvpkkpkee evpekitlkp
    12001 vkkekpeekp keeeipelkp vklekpekve eteekeevmv eitrkkpkkk peevpvekee
    12061 vveteiteit eekpeeisvl pwrrpkkvte kkieveeikp ekvedkevee vtettevswr
    12121 kqrkpkkpie eeekpekvtl kpvkrekpee kpkeeipelk piklekpekv eeteekeevk
    12181 vevtrrrpkk kpeeiqreei evetkevvdd vevekiekqe iqiedrkrkr kqrtqvdisi
    12241 idkdkkiapr fiqklqpvia epestakftc tvfgspvpei swyrneqelh asekyimtvy
    12301 ettasleitk vkeedagmys crasnpagva tstvnlvife keeegiaphf stpikplmve
    12361 ehkpallecv vtgspvpevk wyrgeqelkp kkgreitfnp etgeaklhil epteedetiy
    12421 rvravnkfgr aecranllin nvvkvskpev lrapkitrpl palvaergkp ltlsadfesk
    12481 ptpevkwfrn gvelapsekn viniyentae lfipevtkkd ggkyeirvqn pvgearssgs
    12541 vtvkdrpdkt devkaprfie plqpqivaeg evvimetkve syptasfqwf hetrplestp
    12601 qvrivtqenk silmikeiks eyagtytcra envggsvtct atinlletpw eetvelvspt
    12661 fvkrlspvrv mdgesanltc vvqgkptpkv ewyhdskpik egkeitivqd tegvcslait
    12721 evfpedagey tcravnpvge avcttslive ayeyvpdsei assivatslt tgqsgseedl
    12781 lspketplfd tdteesapei ikklpqlipt kdgeltrlev kvkgkpkpeg kwykqgveiv
    12841 ssqefqieef edgtsvltia etypddtgei vfeahnplgv sttmtylsve givgtkeyrk
    12901 pewvthmeem qealratqtv prfiqeitdv yvkegdtavf ecsysgnptp dvvwykndkm
    12961 lmstqnlkvr iydddkktvl tikhateedd gayvckitse tglatskakl hvtdvtdekk
    13021 fyeeeeevee qtievkpkek pqkkklrkak evkekvkter vkvdkkevrk eklvpkeqpe
    13081 ekkivdieet qilestdiie ekptevsqak kivpiqepvl teataslkki ddekpkdmvp
    13141 kpeerakrvi eeregivvvs evttedmapd ftveskvdra qitseslqtv tvsevhtets
    13201 vqevrraeak qrkakktvvt eeeifeerpk evvveekpkk kkktekvkvr eevtieeive
    13261 rqreniarev eeimemlhak efgpgeaplr elatigylvr qgvsvneine slyrtenfpa
    13321 lktpeaqnal vqlvereghg plitqvltee tttdesvvaa tvgfrafmrm ielqhvtvee
    13381 vlthfapeaf rprawevtea teveteervt ervdvvrkte vhfmerrder kavrtqdird
    13441 ikdvtektve qeiieevkev kptkkvikke kpvkakdevt eevveeiiie epekqeakpi
    13501 veeeetviit ehdvrkapkk kkevvikeee eifetietpt hkiirkkirp mkkgekevvv
    13561 eeiiqkpree tvemeeqqiv evvetptkki vkkkkevvkd dgvpeelvee iiiekpqkkk
    13621 akptveeekk kvkvtkvdtk kapekpeevv eeeeeiieti etptkkiirk kpkdkkdkii
    13681 eeviqktiee akameeqeiv evietptkkv ikkktttiee gkapeekvee iviekpkevi
    13741 eeeeeiieti etptkrivrk kpkdkkdkiv eeviqktiee aktmkeqeii evietptktv
    13801 ikkkkatike gkapeeevee iviekpikek akkkilpkeg veitevitet sveelieeel
    13861 kpeeakpsee veeekvkite vaikkvpkkk kpskaveaee keevveeikp ekpkeekakp
    13921 tvtekegiev tevisevape klpeeakpkk kkateikete ekvitkkvkv keapkeavpg
    13981 eeeqpaeevd elvpekpkkt kakkevtpqd sivttevvpi tkeeelevek peeekatvve
    14041 ekeekakvee kkvtvkkapk kklkpteaee eqveevveei tpeepkkrka kkavipkeei
    14101 qttevipgit aeeipeeekp eeekavpkee ekveeeakvt evsvkkkerk kkekpvtnge
    14161 vieekpkvke ipeekpkeev pqvpeekekp eeekpkevkp kekkpkeekp kkpieekpee
    14221 vkpeeekpev kpeeekpkee kpkkkkpaek kpkiseeerp edkveeipek kpeevktree
    14281 iipkdtvqtt evipetvaee iptkkpkerk avpveekeek veitevpvek apekkeekvp
    14341 eekpteaape kpkeakvmkk kkkkpakkde ieewvepeye rpvlepmpek ivwepkkkke
    14401 kkplpeslqk lvpqkierke mkptklkyse paetvqfasi klkkvavpkk keadetkfpk
    14461 imlrsrmtfv gdyppelqip kityleenpv qtgilsrnte ealkvlkrkv kkvklsekev
    14521 tqlekldlef eelkkvplek iedksvyerq pkkpaekpee pkkvpigkgk vpeeekvepe
    14581 kvklkkvpek kpeeveeplk kpkkpepeee vkpekkkkpk vklehdelkp ldfdrpelek
    14641 yepeeyekpe epapepvpkp yekepkkkpd qeveqiplkk gkpkspepde epevkfript
    14701 kekpeeepeq itlkpwkkek peeekekeip kkeeekvpkp pkeepedvtv qrkaktkkpk
    14761 ekpkeekeee vtvkkpeeek peevaeevtl kkepekpvee apaevtikkp vveekeveee
    14821 etitevilkk kpekpeevve eavvkkpkkk kpvkeeevve evtvtkvvte eekeeekvvi
    14881 kkpkkksipe iteevteeit lkkpeekape evteeikkpk kkkpvkeeva eeitikkpvv
    14941 eekkeeevpe evtlkpkkpe ekpvieevte eitlkkpvpe evteevaikk pkkkkpveee
    15001 aadeitlkkp vvvekeeeei teevtlqkkk kpekkpvpee iseeitlkkp vpkeeekape
    15061 evteeiaikk pkkkkpvkee aadevtikkp vveekkeeet peevtlkprk pkekpvpeev
    15121 keevtlkkpv peekekvpee vteeiaikkp kkkkpveeea adevtikkpv vvekeeeeit
    15181 eeitlgkkkk pekkpvpeei teeitlkkpv pkeeekapee vtekievkkp kkkkpveeea
    15241 adevtikkpv vvekeeeeap aeitlkrrpk ekpveeitee vteeitikkp tpeekekape
    15301 evsekvevkk pkkkkpvvee aadevtikkv vveekkeeea peevtlkprp kkksvteeev
    15361 aevtltkpkp ieeekkpees eltikkkpkk kpvieeeaae vtikkpvpve pdeapeeiti
    15421 rkkkkpekkp kvteeveeta vtikkarape kpteeeeitt dvqikkkkpq kkveevaade
    15481 ltikkieeve qpeeeeevqe ftfkrkppkl ppkpieeiye dvtlrklrpk rksrpdikei
    15541 tetenvtfrp rttktkedve qefkislnty eeedismsgk vrlkpkkkpl tyseetgeet
    15601 iriiqevedd sgpiieeiie sdeegkdeys ieeldtdemr lplrrkkkkv pkpykvedie
    15661 egdvklklkr erkysieead eslalklksk rrvstydeee atlsitreed iseeveieyv
    15721 vrdgdtmfsi csyvaetdea inlvegervy vidhtnqdww fvkkhlteek gwvpaqylln
    15781 evhytlylqr klhekidklp vfekpapgdk ssapkfvekl qpihtpdgyt iqfecqvegv
    15841 prpqitwfrq taiikpspdf qmyydddnva tliirevfpe dagtftcvak naagfasstt
    15901 eliveaplsd hgsdltgpsr kslsressla dilegipptf srkpkakyvd egedvilecr
    15961 lvavpepeit wyykdtqitt keniiiates dmhmycsvik itkvqkkqeg kytivarnre
    16021 geatieipmk vktgkseppe ileplqsyvv regetvvlst qivgnpapkv twfkngkplk
    16081 gltpkqdghv ntltliqpqv sdtgeysvva tndlgtaetk atltveinlk pslainfqqh
    16141 tfehfsvtke ipsgapeppl fterfqeltv pekgtfklva kvtgnpvpev twlrnnkple
    16201 kspnitetyd genivleirn ansevdtgdy kcvasnpvgk ashgarvtvd vdkvtftktl
    16261 ekevvvdeyk tleltcetsh tvstkwwhnd keisgmdhre iieegrvhkl vikktnptde
    16321 gtykctvknq stssnvtvka tkpefvkklq dcevkerdva ileveitsqt vdvkwykdge
    16381 qlgpgkekle fvkegtvrkl lirstsvhde geytcslldq ectaevtvie lppeiitkmq
    16441 dvtiargera tfdieltkgd alvrwfkdgq elqfsehvrl sidgkrqklk iydtesedag
    16501 vyscevgqqk ssakltveep avefitklpd vtlvplnsda vfvielsqdv pvtwmrktqv
    16561 ikespkytii degkvkkliv kkcttedise ytamvanvkt ssrlrvevie tppkispdtp
    16621 rkykvrkded vdivvkfsgt pkpedewtvn grvvtkskra iptideesav ltirkiqeed
    16681 vgdytlklvn svgetsiein vvivqvpsap gapeplettn dsvtlhwkkp dsdgnspive
    16741 yilehqekte ttwtkiteti tetthkvtkl ttnkeytfrv tavneagpge tsptspyvki
    16801 skpstaeppv ileplksivi glgetvtlsc viggtpapei twlrndeife dtsityenhi
    16861 tkytirette tssakftvka knnvgsaett celkiqespk iiydenltnq klpmngqwki
    16921 eiqitgfpkp evtwtkngke vdekrvsihr eertstvsis slvredtaty makaenqags
    16981 ssvdlhlkvi dkpskpqgpv vfkeirqdrv tlewrppadd ggielekyti ekcepgktwv
    17041 kvvdidkeve sfcvhklqqn aeykfriiar navgaseple sdtvkmrtsf eppgaprgpl
    17101 evsgmtktsf tikwqppend ggtpitdyii eikeeskktw qkigstkhet thfsvsdlkt
    17161 dvpynfrita knsvgsgppy vaeepiapgr rikitkltes smyallgifp ptkivisktp
    17221 psspqhvhvt nitsksatls wsppastggt eltgyviekr pligkgarwt kvvtldattl
    17281 qycvenlkes eflfrifaen nqglsvpans epvtlkthas vpspptaple irqiaantvv
    17341 iswgrpdsdg gaplegykva irdtkrtmwm evarvnteiq klnirdlqen hmylirifar
    17401 neiglsdple seepfkiipa seltvvepia eatekgetas isfstentss wlrehnmdad
    17461 ihsyararll rqdeyffriw hyakklfe
//
LOCUS       XP_034182986           10006 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X22 [Osmia lignaria lignaria].
ACCESSION   XP_034182986
VERSION     XP_034182986.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327095.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182986.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..10006
                     /product="sallimus isoform X22"
                     /calculated_mol_wt=1126114
     Region          416..600
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cl02488"
                     /db_xref="CDD:413338"
     Site            495..500
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1152..1376
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            1255..1260
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          <1557..2211
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          2383..2472
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2413..2417
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2438..2442
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2452..2457
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2465..2468
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2484..2573
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2501..2505
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2518
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2539..2543
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2553..2558
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2566..2569
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2583..2672
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2600..2604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2613..2617
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2638..2642
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2652..2657
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2665..2668
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2695..2784
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2712..2716
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2725..2729
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2750..2754
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2764..2769
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2777..2780
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2810..2900
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2827..2831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2840..2844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2866..2870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2880..2885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2893..2896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2922..3010
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2939..2943
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2952..2956
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3003..3006
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3178..3267
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3195..3199
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3208..3212
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3230..3237
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3247..3252
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3260..3263
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3274..3364
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3291..3295
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3304..3308
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3330..3334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3357..3360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3801..>4494
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <3999..4270
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          4985..5074
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5002..5006
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5015..5019
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5040..5044
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5054..5059
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5067..5070
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5231..5318
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5248..5252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5261..5265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5288..5292
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5302..5307
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5428..5517
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5445..5449
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5458..5462
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5483..5487
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5497..5502
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5510..5513
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5553..5643
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5570..5574
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5583..5587
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5609..5613
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5623..5628
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5636..5639
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5652..5742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          5669..5673
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5682..5686
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5708..5712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5722..5727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5735..5738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5799..5890
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5816..5820
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5831..5835
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5857..5860
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          5870..5875
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          5883..5886
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          5932..6024
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          5950..5954
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          5965..5969
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          5990..5994
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6004..6009
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6017..6020
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6071..6163
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6089..6093
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6104..6108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6129..6133
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6143..6148
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6156..6159
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6205..6297
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6223..6227
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6238..6242
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6264..6268
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6277..6282
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6290..6293
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6339..6431
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6357..6361
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6372..6376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6397..6401
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6411..6416
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6424..6427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6473..6564
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6490..6494
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6505..6509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6531..6534
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6544..6549
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6557..6560
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6606..6696
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6624..6628
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6639..6643
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6664..6668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6678..6683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6691..6694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6740..6831
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6757..6761
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6772..6776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6793..6801
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6811..6816
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6824..6827
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6873..6964
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6890..6894
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6909
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..6934
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6944..6949
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6957..6960
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7006..7098
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7024..7028
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7039..7043
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7064..7068
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7083
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7094
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7140..7232
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7158..7162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7171..7177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7198..7202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7212..7217
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7228
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7272..7364
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7290..7294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7303..7309
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7330..7334
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7344..7349
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7357..7360
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7405..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7423..7427
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7436..7442
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7463..7467
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7477..7482
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7490..7493
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7539..7631
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7557..7561
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7576
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7601
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7611..7616
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7624..7627
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7763
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7689..7693
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7704..7708
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7729..7733
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7743..7748
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7756..7759
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7805..7897
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7823..7827
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7838..7842
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7863..7867
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7877..7882
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7890..7893
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7937..8027
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7954..7958
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7969..7973
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7994..7998
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8008..8013
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8021..8024
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <8104..8164
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8108..8112
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8133..8137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8147..8152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8161..8164
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8212..8303
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8230..8234
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8245..8249
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8267..8271
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8284..8289
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8297..8300
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8313..8404
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8331..8335
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8346..8350
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8368..8375
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8385..8390
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8398..8401
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8446..8537
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8464..8468
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8479..8483
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8501..8505
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8518..8523
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8531..8534
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8584..8675
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8602..8606
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8617..8621
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8642..8646
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8656..8661
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8722..8813
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8739..8743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8754..8758
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8779..8783
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8793..8798
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8806..8809
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8859..8949
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8876..8880
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8891..8895
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8916..8920
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8930..8935
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8943..8946
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8992..9081
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9009..9013
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9024..9028
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9049..9053
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9063..9068
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9076..9079
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9135..9225
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9153..9157
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9168..9172
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9190..9194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9207..9212
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9220..9223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9273..9361
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9290..9294
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9303..9307
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9327..9331
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9341..9346
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9354..9357
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9596..9686
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          9613..9617
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9626..9630
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9651..9655
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9665..9670
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9678..9681
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9700..9789
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9717..9721
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9730..9734
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9755..9759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9769..9774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9782..9785
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9810..9899
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9827..9831
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9840..9844
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9865..9869
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9879..9884
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9892..9895
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          9911..9998
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          9925..9929
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          9939..9943
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          9964..9968
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          9978..9983
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          9991..9994
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..10006
                     /gene="sls"
                     /coded_by="XM_034327095.2:129..30149"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mptksveave ilealeskla yipggrdpeg rplivvnvps elqpntkpwl ealiayflsi
       61 fseetkkagl vlivdarrga wrvarscirl smllfgsfaa svivlrpdgf wdkrvdsctk
      121 shkegeptyl tltrlhlhvd faqlpyelgg nklydhaewi qkrvkiedyc keasdlvskm
      181 tnlhqrlisl dgfrmsesdd dlesywgmss elisiarhil qsgrnlvssm sretakdild
      241 ttrrihelid sielkqvdie nswsemernl dtarvigdle kgvsrvtdwi lgpadamlns
      301 ccqvgfdvss seelrrrhee lelecretyg ryaellhkid slpstrlsed lksqrdfmdf
      361 vcrsfanrle rrrnvlitsq rffrlvseyf dktsevfdkl vmgnrthdfs qatanlmkle
      421 qsqailetle relvkegekl sdilsmpvkd algrdvqvdy gedivnvrdi ldatnarkni
      481 fndsvelqkl slkqialiyt yendaeqavk wlndlyevll knhmeigcna ieiqsqkkeh
      541 qsfqetakgt ydygcqlvng arvlrlscrl plesnaslfs rlrqawkqlr svgqeqltrl
      601 rvcavfhrsv edhcsklrdl ieavgllrrs sriedlaaes raraeirdil gnreklllev
      661 grmvrlgrll rtrlkeplch qrmseaedas gkgsnltave aisarlaevt rlaekldarl
      721 ceagartrpv pmsssamsst sslfsssnqp tttslaatsv qsqpisagis sitsmplict
      781 sestvpfgtk gvctvlatks svasskpver stspvqvngg isggtgeava gsepskpekk
      841 iveedtremg seiyrdased kiddsqeekl keeiedimek scegsladgs niedyvtate
      901 csitptarsr sesfvttpec eelaatvaat sasdicwnie epkfissnaa vssslikqee
      961 askgqkvers nasmesfkpd iyetngerqv eklvsmekrv deksgkvvke vtetttlrvs
     1021 hdtrlgiasy kvisnttqdh rekvdvkeik dierivspdv hektnglhhd tetgsktdsy
     1081 trrdelstkt ddtertikft kicevsqeek prvvsgdtsk dmtlflkeyt rhhedsgiem
     1141 sptkkeetle cqdflenark sgewlrlkvl evqpeltklg asveeatels sahdevllrl
     1201 qskqspveel lrqadqlisn qrpraevyaa maetlgqawr dvnellerrk qildcnvlfq
     1261 craeecresm ralemacndt llpieieavk nflskihdlr knmlealmga lqegknlldr
     1321 lkeianegtl dsrpdrikle adhavlkvek wleelhdrrr lieasfrsrk tqleqclala
     1381 llatdlrdle eilndriaal ssscdqlgds assaelllfe lkklqaeake fqdrsikitk
     1441 sterlvssgh fageqateqa yailgaaady vndldqyesl lnravaffds arsaltkldq
     1501 leiqlvtteh pqystrlarf haqtvatied vtakplaegy alldvtgrga pgaegvkrtv
     1561 edlenrkirl merctaheke nleisrmint flekhdelrk wlmsipeafl qghqdmgsdv
     1621 pmaedfcrlh rqlsndlerr tdevehlefe ilpireklee sqklelkskv eelqrswskt
     1681 kelvanridl gsvylqfhmv adeltreves ieselksnad vlderkveel grrwkdlqpl
     1741 yvkltgsgka fldaaikidd pyldvpracl cvqtllekfa nrqltvtesw ekwrtsieil
     1801 rerrieherk ieestrtlew vskfseqlyp vitsqsaett milkdlagsk qrilpelnka
     1861 vseldsrikg intlaqkgev hvdeeilkrl hqmhedlrkt ardyeilles lisifesiee
     1921 veqkietlki qvervtslkk ifeveaafse idatkwsisd rlghiklrie daivrikqqe
     1981 ppgagvkdie klrraldslt sdfeifwsqt ttkveehrrt ctfiedleri dgelknlneh
     2041 lknvdarmge nlqtakatsa sfvqfektvt ileqriekfv qtteesikil tpqivddisl
     2101 lkerwrnlkk rmeetkkrmn lcidyfvlle eakewnregs kllvviarka tmvkapkdat
     2161 dllheienyl kpgeevqerr ieklkelsti vfgterlpqf nevivenrqm ldsftvvsse
     2221 lrtlvqnlkn aedlqeklri ekqeadeklq aakvemaaaa aareeaenar kiaeklaaet
     2281 lekaatevkt lkeereaqtm sappsfsvsa qtekiektde syvqetvtsa vttkeihilq
     2341 kteiektvpl iqrepspgki ivteeiheks ekiekddvpp lapeftipln datvqegekf
     2401 sfqcnlvghp lpevvwykdg isilnnpdyl ttyvrdvctl tieetfaeds akytcrafni
     2461 agsaetsatl tvketapeeq psppvfvkel vpstaiegss hqidcvvegn plptvqwfkn
     2521 dvnidnspdy vitynngeav lkfeevlped kaiymckatn rlgqastsas lnvqrtectt
     2581 ekpqfvtpls namgragqrv kleceargkp mptltwyhdg rsveetmnlk iqtdggrssl
     2641 iiseafakda gcymvvaknd ageatvscnv svkgrlphet sdsefacsdm epvvpkiqmp
     2701 lkdlrvqegr svrldcvivg qpepeviwyh ddqpvkesad fqllfqgdkc tlliheafld
     2761 daglykvvai nsggeassqc tltvtpvsvt helektrtev eetvtagspp kfvklptdsl
     2821 iaegenaife cavvgepkpe lkwfsdsgei seseririqr kedgtsilri lsaipedkgn
     2881 yvvkafnahg eakafarlvv rslgdfrrke efvqmeekli aptfkerfed rkvpdgvstk
     2941 fecivvgkps pkiqwlfndr pvhgkdflvs vsgdrqvlti petgsthvgt iscvaenaag
     3001 katctarlei ggsevqrdkk evveekvyel veripgdemh assssdkhfs aekseiggen
     3061 kvltkmseti tesstthktl kkeyisssms sstgapgqep tsvcvkstvh steqsssadg
     3121 appvvqsykv eehekiiqdq pgeirqertv vvsqdeegik rdikatqipk pvrkstaprf
     3181 vspvtgmivd qgsdivlegi idgfpqpvvi wskngqelkt kdsmtitysh nhvrlelknv
     3241 nvkdagrytc tasnevgsas stadlvvkkt ifppvfgrrl qaqlvkrgdr vnmeveitgt
     3301 peptvswfkd dipikerppe lrikqqgncy mlimekaake hagkymvrat naggeaqsia
     3361 dfavfeptpd tmvevhktvi ydvqdkklmq sdekkaevps skhltseqvs ttkiqpaati
     3421 kttptamsts maqmsrtvek ikepdtelcr sekisstves hqmerkseqk yhmklehkmp
     3481 sivepktegk tvikeviree vrssepkstl ieekieneni etsriakkda lsffesmtre
     3541 tetipkgpke miklvddadg hevkvgkltk nyekstsfqe vkkpevkppe llttkkavhe
     3601 iftkleqgsi psrgidnklf dfpyepykpt metkktvvee tissgssslq ssmtmskmes
     3661 kreskmesks sellsegfnl vpepppeigy mpkpeevkkk rpevsikakq lqesfdktls
     3721 pidapiggvk ifpspspkil etpkvappsf smppppfqle kkeiveevci kkdvhekrdp
     3781 qplvrpisps rpwssssdve trsqmstdls eyrchsaass hqeilrsssp rpsadglame
     3841 kswakkcads trkswppqed tpikykqewq mpgedyktss kefkreveet peggikktsm
     3901 essstterrs wsskqetmdk mskrapsppk iqpiiynaet ikvdhtvnki eekslyekyi
     3961 secdvkksqk sekleeyssk kwtgaedlka pglvrsletp kkptpplyrp vepiptdqii
     4021 lepgpppeig yipapvvrek kieqiektle vslehkpaki ppggirtipp apkkeeppal
     4081 ppkdvritpp plpakqptpv eplepfpfkp spsptiktpk vavpaptpsk fvkgsfgves
     4141 dyesdmdshm kakwrpyesd neepryrrvq ppvpkqprpr stepeplpps kfevpsestg
     4201 lprpvltkeq qekmykkttt fkrqeketkq qkyqvvqppp velkpgsppi yvqpatkspt
     4261 pksppakkpe spkfkvktfq qesgymadtd eplqqkssva slsksfgkqe ssssmsshme
     4321 srtsysesrs eyfesksyds rqqqqkkesf apsapssvkq ppqqrssfve ksygssavts
     4381 sprfmsaket lyttdqshkk feapkkilpp spspsrfvks efresdyesd ydgripplwk
     4441 prgyesddqs frtvkpnlag pgkpkvqred tsrlieeast raqkaktfek reervatkpk
     4501 tvllpgsppe iayappqpqp tyyearsgip fhnaigtetk ktvrmdeste nsrrivtveq
     4561 tsrvikfgdn qksatdfdsf dsssaqkqkt hykvptpkkf vqgqfresdy esdidtrikp
     4621 kwappesdse epryrkvqpp ttkttrsssa pvrqehvvsp mefdtgptvl rkqtsmsqlr
     4681 derkfdgqvk rqassrqeet lkpgsppefg yisrsdvkka anhvasrhms dmtssfkskt
     4741 ekfvtdiqsd lkqgkpilkq pvdgrasdad eprtyreesr lsehgtkqid pdtgliyfky
     4801 dfgyefgivl pgegkktvss tsrsvqgqrr asdievpivh efttrkengf akrsasssst
     4861 arqgkpagkf sgsktvkwep tseseysese dirnarkrhs eggntmtaps lvipcspsps
     4921 rwdhttpspl slspslpsls pryssatgpp snvdsagspw pttngvassk eviqpyleil
     4981 pkkaplfitp lrdiavvsgq tarfecivqa epqpnilwsk dgriiensts yeihyrngvc
     5041 rltivrafpe dagtyactat nslgstvtsa tllvpgvpsp fygtyrdqnl sersaesppk
     5101 yvalrdlflp qesdvktass nrqqqhnsst feyawsvnrs yaggryreke nateecveit
     5161 srnpiarrle ysdfqandsf rdvadfcfdr eystcnpsia sprntvqgei vmqttptvpa
     5221 ftgrpgdpsp pvfeqifkna rfaqggnaif egrvrgnpkp tvtwthkgap lleswkirms
     5281 yddktgavtl qinqigpgde geytcsaknq ygeaicsvyi qpegfgpppq qqiggyrkey
     5341 aqtfqsteqk sqtgsqsfqq rsyqqtidkr syvngtstsi edfkvdtfey rllretefre
     5401 sitrrfvges dvqistvvdr slgpvappqi sqkprnskli egsdavftak isgnpkprlt
     5461 wfkngqrire sqrvemshsn qqatlrirva lpedsghytl lsenpqgctv ssaylaiess
     5521 dqvdqayqtp resvktpqve stgesdsgkv lppnfvrtct drdategkmt rfdcrvtgrp
     5581 ypevtwying qqvandlthk ilvnesgnns lmitnvsrad agvvtcvarn kagetsfqcn
     5641 lnviekeqvv apkfverftt tnvkegepvv fmaravgtpv pritwqkdgv pitpgpdvri
     5701 stdgsgastl dipcaklsda awyqctaqnv agstatrarl fvetpkgaap eprrlnlprp
     5761 tkviepepap gpeviylrhv erakpylppp eedrvypppr fiiplrdvhq ieggrihfea
     5821 riepvgdptm rvewyvngra ldassratsi frfgfisldl isiilqdsge yvcrvvsstg
     5881 vaesratlsv tpratieqas qhpdslqyiq qledyskyqr qesveeissq rpafirplqd
     5941 lgelqegrna hfeaqltpvs dptmkvewyk dgrpitassr ittifnfgyv slnilhlrae
     6001 dagsytvrav nrlgeavssa slrvyartsv ttdlgipeqq ryieaaeele ayqqamhqky
     6061 vqeqpeptsp pefkspikdq nsireggfah fearlepvgd sdlrvewlkd grpveassri
     6121 ttffnfgyva ltikyvtihd vgiytcrayn rageahttaq lsviskndvi ydsqhptglq
     6181 kiqtledssr ysrqvqeetq vtqaprflgt lkgtnkiveg qrahfearve pqsdltmtie
     6241 wyhngkaita anriqtyhdf gyvaidilqv rpedagtytv varnalgear lsatmvvetr
     6301 ssvdtssmhr tsyektqrle eskfvepqyh ieeiskskpi fvqplsdpkp vsegknihle
     6361 crlepmgdpt mrvewfqngr pvtvgsrfrt yydfgfvald ivhstildsg eytvratnhl
     6421 gtahtsacvr vigksdivte tqheqsleqi qmledaaryr ktqqeevtvm qapqftrplh
     6481 nietveltnv hlecrlqpvg datmkvewfv ngrpvktghr frpsyefdyv aldilgvype
     6541 dsgvytcqar nqlgeavtsc svrvhakkdl llesqhpegl eriqyledas rykrhelvde
     6601 vvkvkprfvt apknqenlhe gqhahfeckl epvtdsnlkv ewykngrpvt ighrfrpihd
     6661 fgyvaldvid liaedsgtyt cravnlvgsd evscslscrs taqvltdtkn eqgleqihyl
     6721 edrskyqrre dveetttqap ifttslnnve ikegqrahfe crlipvsdat mkvewfhnnk
     6781 pvkagsrfie tnsfgfvald imyaypedsg tytcraknii geaitsatan vhskksiyte
     6841 tqneetlqrl hhledtsryq rkttteeivt qapvftmpik dlkvaenqaa hfearvipvg
     6901 dsklkvewlr ngvpiaasnr vttmhdfgyv alnmkyvnpe dsgtytcrav ndlgeavtsa
     6961 tlfvqskaal qfesqhesal sklqaledts ryqrheeeei vvkekpsftv qlngptnlve
     7021 gqsahyecri epypdpnmkv ewfhngkpls tghryrttcd fgfasldvlt vyaedsgtyt
     7081 ckatnklgsa qssikldvks rasiiretqh esalkkiqyl eddsrykrvd qedliiaekp
     7141 kfgrplknie hlpegksahl eatltpvndp tmvvewfrdg rpipqghkfk ttydfgyval
     7201 dilyaypeds gtymckarna vgeavttcvi svdskqglfl dtldaqrlqk ireletvevk
     7261 qevekevvhq kpvfltplnn ldhlkegeha hlecrvepin dpnlkiewfv ngvavktghr
     7321 frkthdfgyv aldilytype dsgtymckat nlageavntc tikvgsrrsi lldtqhpdgl
     7381 ekireleaqg hparleveep pvtpprfvte lrgttevyeg qtahfecqie plhdanlrie
     7441 ffhngkplps asrfhvtfdf gyvaldight vpedageysv rainalgqcv ssielrvipr
     7501 dniildsqrp egldkirele aqqpwkrpdi pepqtrqrpv ftqplqnida ipegqtahfe
     7561 crlipvgdpt lkvewfrnei pletssritk vhdfgyvsld inhvreedeg vymcrasnpl
     7621 geavttasmk ikskasiqld tqhpeaqrki aqleadkpsr peepekvfdk piftqlltgp
     7681 telwegqmar yecrvvpvgd aslrfewyin gvelkmgsrf hvshdfgyvt ldilkvited
     7741 sgvytckain kageavssis lkvkarsaid adsiqpdawq kiqlkeaemn kvpemfvdtt
     7801 pqqapvftkh lesydklveg qhvyleaqve pradpnlrve wfkngislqt gtrlrstfdf
     7861 glvtlsingl rpddsaiytc katnllgeav stcslkivdr hwllgdtlhp dalpkidale
     7921 qphvtsaeqp epiyeepvfi thlnnvecve gdnvhfecnv epskdptmki ewfingkplp
     7981 tgarfkstyd fgyvaldith ayeedsgvvi vkatnskgsa qtsgtlkcts kqsiylqtqh
     8041 pqgeaglekv keaedaylsk yrrpdegpeh eypkpiwtvp lqpefklgef eplhlegqve
     8101 pkddpnlkie wyfngkvleh asrfkmtsdf gfvtldltdv yerdsgiytc kaynkageaf
     8161 tsttiycstk eniiersqhp kgkegleaiq dleeslkrqe gappeseegh ppvftsqfen
     8221 ltnlsegeia hfeasltptg dqtmvvewfy ngkvleashr irtvyafgmv vlevlgtkie
     8281 dsgtyscrat nkwgqaeisv elqcvdkskg qkpkftthiq sleglkdgqs ahfectlipv
     8341 gdpqmkvewf hngqplrhss rfkmvsdfgf vvmdiagvma hdtgeyvcka snkygedftk
     8401 atlkcfgksg vyldslqpds larirelesf ggeqpttptt pvaeppkfit qivditrlve
     8461 gqsahfearl tpvndpdlkv ewyyngkklp hghryrtfhd fgivildily cyeensgvye
     8521 crafnkyged ttkatlkcfs kaslilesql pkgmegglek iqtledsmir tkdekvveer
     8581 gkapmftvpl snidglrege sahfearlip tddpklkvew fwngkplrtg srfrtfcdfg
     8641 fvileispiy pedsgeyscr atneygeavt tctmkctgkr siilesqlpk gmestidkia
     8701 eleglgnepg qvtpeddtgk ppefvttpsd ltltenslah fecrltpind ssmrvewfhn
     8761 gkpllagsri ktihdfgfvi levancyqrd sglytckatn rhgeatvnck lqvrgrqgii
     8821 lepqlptnfk tgtesiqkle ealykrdeil teeetpnppk ftvelkdiev eegapshfdc
     8881 rvepvgdstm ridwfhngrs fatgsrvhqi ndfgfisldm sytyardsge yvcratnkwg
     8941 sattkatitc kskktidfds qlpsgmsgek lkelergpvs eppveeaprq pprfithiqs
     9001 atvdesepvr fecrvepkdd pnlriewyrn gkmipaghry rtmydmgfvs mdilyvyped
     9061 sgeyvckain dlgedttras lsckklpsii lqnqvpkgmk ksealmqmea tikkytsevh
     9121 lteddlydad kkqpprfvtq ildqtelvem nsskfecqla pvgdpnmkve wffngkplph
     9181 knrftpiydf gyvamnfgwv ypedsgeylc ratnlygmde travirtagk pgiiyesqlp
     9241 kgmksiekir emeaawqivp eeegeeekvr apptfvskpe pvtveegdcs rfccrvtghp
     9301 rprvmwiing htvvngsryk ltydgmyhld ipktrqydhg kveviarssv gesrtettlt
     9361 vkprsddyrg vlknsprpwy dygltqyqte rqntelekvf derhhnvsqg ieiatehlgq
     9421 kvfkepetdw qksvkskkne eyynklmtle eeqvlketrl resshqfaip gekvvadsva
     9481 kgmaqkyeet leekkdektq etvqppprpl kkphttevdi dvermkvtgk yppgpsestv
     9541 hgrevhvakq kqtqkevkgd leitrkitat ettevehkak tqervvqgpt kpakppvftk
     9601 kiqpcrafeq eqarfevefd gdplptvkwy redfpiqnsa dlqiytfstk svlivrqvfm
     9661 edsgvfsvia enrggkakcs anlvveerrr qpgrggvipp sflstiqnts vttgqlarfd
     9721 akvtgtkpld vywlkngkki tadiryktle edntytllll etvpedsgky ecvainkage
     9781 arcdaectvr gpqspaksak pttpgvekap avleplkdqt iregtsvafs crisgkpvpt
     9841 vqwkkgdkvi kpskyfqmsk egdfctlris eafpedegvy kciaknpagd vttsanlrvl
     9901 apdaadvlpk ltplkdqivs egqpaqfktq vtpskpkpti qwyregalip qspdfqmihe
     9961 gnnavlliat tyeedtgtft crattsagtv etsaklivkk calllk
//
LOCUS       XP_076546014           18862 aa            linear   INV 03-SEP-2025
DEFINITION  sallimus isoform X23 [Osmia lignaria lignaria].
ACCESSION   XP_076546014
VERSION     XP_076546014.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689899.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18862
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..18862
                     /product="sallimus isoform X23"
                     /calculated_mol_wt=2134329
     Region          74..161
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          91..95
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..108
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          131..135
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          145..150
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          271..360
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          288..292
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          301..305
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          326..330
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          340..345
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          353..356
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..486
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          413..417
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          426..430
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          452..456
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          466..471
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          479..482
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          495..585
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          512..516
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          525..529
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          551..555
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          565..570
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          578..581
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..733
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          659..663
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          674..678
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          700..703
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          713..718
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          726..729
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          775..867
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          793..797
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          808..812
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          833..837
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          847..852
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          860..863
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          914..1006
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          932..936
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          947..951
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          972..976
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          986..991
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          999..1002
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1048..1140
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1066..1070
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1081..1085
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1107..1111
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1120..1125
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1133..1136
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1182..1274
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1200..1204
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1215..1219
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1240..1244
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1254..1259
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1267..1270
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1316..1407
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1333..1337
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1348..1352
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1374..1377
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1387..1392
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1400..1403
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1449..1539
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          1467..1471
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1482..1486
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1507..1511
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1521..1526
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1534..1537
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1583..1674
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1600..1604
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1611..1619
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1640..1644
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1654..1659
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1667..1670
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1716..1807
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1733..1737
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1748..1752
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1773..1777
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1787..1792
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1800..1803
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1849..1941
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          1867..1871
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1882..1886
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          1907..1911
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1921..1926
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          1934..1937
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          1983..2075
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2001..2005
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2014..2020
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2041..2045
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2055..2060
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2068..2071
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2115..2207
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2133..2137
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2146..2152
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2173..2177
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2187..2192
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2200..2203
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2248..2340
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2266..2270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2279..2285
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2306..2310
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2320..2325
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2333..2336
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2382..2474
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2400..2404
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2415..2419
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2440..2444
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2454..2459
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2467..2470
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2514..2606
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2547..2551
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2572..2576
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2586..2591
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2648..2740
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2666..2670
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2681..2685
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2706..2710
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2720..2725
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2733..2736
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          2780..2870
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          2797..2801
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          2812..2816
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2837..2841
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2851..2856
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2864..2867
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <2947..3007
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2951..2955
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2976..2980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2990..2995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3004..3007
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3055..3146
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3088..3092
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3113..3117
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3127..3132
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3156..3247
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3174..3178
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3189..3193
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3211..3218
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3228..3233
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3241..3244
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3289..3380
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3307..3311
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3322..3326
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3344..3348
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3361..3366
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3374..3377
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3427..3518
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3460..3464
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3485..3489
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3499..3504
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3565..3656
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3582..3586
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3597..3601
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3622..3626
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3636..3641
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3649..3652
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3702..3792
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3719..3723
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3734..3738
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3759..3763
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3773..3778
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3786..3789
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3835..3924
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          3852..3856
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          3867..3871
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          3892..3896
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          3906..3911
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          3919..3922
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          3978..4068
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          3996..4000
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4011..4015
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4033..4037
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4050..4055
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4063..4066
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4116..4204
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4133..4137
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4146..4150
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4170..4174
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4184..4189
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4197..4200
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4439..4529
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          4456..4460
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4469..4473
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4494..4498
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4508..4513
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4521..4524
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4543..4632
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4560..4564
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4573..4577
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4598..4602
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4612..4617
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4625..4628
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4653..4742
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4670..4674
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4683..4687
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4708..4712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4722..4727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4735..4738
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          4754..4841
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          4768..4772
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          4782..4786
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          4807..4811
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          4821..4826
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          4834..4837
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <4918..5615
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          5425..>5834
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          7092..7181
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7109..7113
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7122..7126
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7147..7151
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7161..7166
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7191..7282
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7208..7212
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7221..7225
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7248..7252
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7262..7267
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7275..7278
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7297..7386
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7314..7318
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7327..7331
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7352..7356
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7366..7371
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7381..7384
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7399..7485
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7416..7420
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7429..7433
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7454..7458
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7468..7473
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7481..7484
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7502..7592
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7519..7523
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7532..7536
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7558..7562
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7572..7577
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7585..7588
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7640..7726
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7659..7663
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7672..7676
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7698..7702
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7712..7717
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7764..7855
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7781..7785
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7794..7798
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7821..7825
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7835..7840
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7848..7851
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <11343..>11495
                     /region_name="PspC_subgroup_1"
                     /note="pneumococcal surface protein PspC, choline-binding
                     form; NF033838"
                     /db_xref="CDD:468201"
     Region          17149..17201
                     /region_name="SH3_p47phox_like"
                     /note="Src homology 3 domains of the p47phox subunit of
                     NADPH oxidase and similar domains; cd11856"
                     /db_xref="CDD:212790"
     Site            order(17154,17156,17162..17163,17180..17182,17195,17197,
                     17199..17200)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212790"
     Region          17237..17327
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17254..17258
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17267..17271
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17293..17297
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17307..17312
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17320..17323
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17361..17454
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          17378..17382
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17391..17395
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17416..17424
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17434..17439
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17447..17450
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17462..17548
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17479..17483
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17492..17496
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17514..17518
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17528..17533
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17541..17544
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17559..17649
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17576..17580
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17589..17593
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17614..17618
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17629..17634
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17642..17645
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17662..17739
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          17672..17676
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17683..17687
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17708..17712
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17722..17727
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17743..17828
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          17760..17764
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17772..17776
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17798..17802
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17812..17817
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17821..17824
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          17833..17917
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          17850..17854
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          17862..17866
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          17887..17891
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          17901..17906
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          17910..17913
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18023..18103
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          18031..18035
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          18044..18048
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          18069..18073
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          18083..18088
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          18096..18099
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18107..18198
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(18107,18172,18187)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(18188..18189,18191..18192)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          18209..18295
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          18226..18230
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          18239..18243
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          18261..18265
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          18275..18280
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          18288..18291
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18319..18389
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          18319..18322
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          18331..18335
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          18355..18359
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          18369..18374
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          18382..18385
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          18393..18485
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(18393,18459,18474)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <18463..18752
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(18475..18476,18478..18479)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     CDS             1..18862
                     /gene="sls"
                     /coded_by="XM_076689899.1:537..57125"
                     /db_xref="GeneID:117605489"
ORIGIN      
        1 mkrqtsqqqq qtmqqqqqtt tqqqqqqqyi qqrterqvtr qqitsqqrvq geivmqttpt
       61 vpaftgrpgd psppvfeqif knarfaqggn aifegrvrgn pkptvtwthk gaplleswki
      121 rmsyddktga vtlqinqigp gdegeytcsa knqygeaics vyiqpegfgp ppqqqiggyr
      181 keyaqtfqst eqksqtgsqs fqqrsyqqti dkrsyvngts tsiedfkvdt feyrllrete
      241 fresitrrfv gesdvqistv vdrslgpvap pqisqkprns kliegsdavf takisgnpkp
      301 rltwfkngqr iresqrvems hsnqqatlri rvalpedsgh ytllsenpqg ctvssaylai
      361 essdqvdqay qtpresvktp qvestgesds gkvlppnfvr tctdrdateg kmtrfdcrvt
      421 grpypevtwy ingqqvandl thkilvnesg nnslmitnvs radagvvtcv arnkagetsf
      481 qcnlnvieke qvvapkfver ftttnvkege pvvfmaravg tpvpritwqk dgvpitpgpd
      541 vristdgsga stldipcakl sdaawyqcta qnvagstatr arlfvetpkg aapeprrlnl
      601 prptkviepe papgpeviyl rhverakpyl pppeedrvyp pprfiiplrd vhqieggrih
      661 feariepvgd ptmrvewyvn graldassra tsifrfgfis ldlisiilqd sgeyvcrvvs
      721 stgvaesrat lsvtpratie qasqhpdslq yiqqledysk yqrqesveei ssqrpafirp
      781 lqdlgelqeg rnahfeaqlt pvsdptmkve wykdgrpita ssrittifnf gyvslnilhl
      841 raedagsytv ravnrlgeav ssaslrvyar tsvttdlgip eqqryieaae eleayqqamh
      901 qkyvqeqpep tsppefkspi kdqnsiregg fahfearlep vgdsdlrvew lkdgrpveas
      961 srittffnfg yvaltikyvt ihdvgiytcr aynrageaht taqlsviskn dviydsqhpt
     1021 glqkiqtled ssrysrqvqe etqvtqaprf lgtlkgtnki vegqrahfea rvepqsdltm
     1081 tiewyhngka itaanriqty hdfgyvaidi lqvrpedagt ytvvarnalg earlsatmvv
     1141 etrssvdtss mhrtsyektq rleeskfvep qyhieeisks kpifvqplsd pkpvsegkni
     1201 hlecrlepmg dptmrvewfq ngrpvtvgsr frtyydfgfv aldivhstil dsgeytvrat
     1261 nhlgtahtsa cvrvigksdi vtetqheqsl eqiqmledaa ryrktqqeev tvmqapqftr
     1321 plhnietvel tnvhlecrlq pvgdatmkve wfvngrpvkt ghrfrpsyef dyvaldilgv
     1381 ypedsgvytc qarnqlgeav tscsvrvhak kdlllesqhp egleriqyle dasrykrhel
     1441 vdevvkvkpr fvtapknqen lhegqhahfe cklepvtdsn lkvewykngr pvtighrfrp
     1501 ihdfgyvald vidliaedsg tytcravnlv gsdevscsls crstaqvltd tkneqgleqi
     1561 hyledrskyq rredveettt qapifttsln nveikegqra hfecrlipvs datmkvewfh
     1621 nnkpvkagsr fietnsfgfv aldimyaype dsgtytcrak niigeaitsa tanvhskksi
     1681 ytetqneetl qrlhhledts ryqrktttee ivtqapvftm pikdlkvaen qaahfearvi
     1741 pvgdsklkve wlrngvpiaa snrvttmhdf gyvalnmkyv npedsgtytc ravndlgeav
     1801 tsatlfvqsk aalqfesqhe salsklqale dtsryqrhee eeivvkekps ftvqlngptn
     1861 lvegqsahye criepypdpn mkvewfhngk plstghryrt tcdfgfasld vltvyaedsg
     1921 tytckatnkl gsaqssikld vksrasiire tqhesalkki qyleddsryk rvdqedliia
     1981 ekpkfgrplk niehlpegks ahleatltpv ndptmvvewf rdgrpipqgh kfkttydfgy
     2041 valdilyayp edsgtymcka rnavgeavtt cvisvdskqg lfldtldaqr lqkireletv
     2101 evkqevekev vhqkpvfltp lnnldhlkeg ehahlecrve pindpnlkie wfvngvavkt
     2161 ghrfrkthdf gyvaldilyt ypedsgtymc katnlageav ntctikvgsr rsilldtqhp
     2221 dglekirele aqghparlev eeppvtpprf vtelrgttev yegqtahfec qieplhdanl
     2281 rieffhngkp lpsasrfhvt fdfgyvaldi ghtvpedage ysvrainalg qcvssielrv
     2341 iprdniilds qrpegldkir eleaqqpwkr pdipepqtrq rpvftqplqn idaipegqta
     2401 hfecrlipvg dptlkvewfr neipletssr itkvhdfgyv sldinhvree degvymcras
     2461 nplgeavtta smkikskasi qldtqhpeaq rkiaqleadk psrpeepekv fdkpiftqll
     2521 tgptelwegq maryecrvvp vgdaslrfew yingvelkmg srfhvshdfg yvtldilkvi
     2581 tedsgvytck ainkageavs sislkvkars aidadsiqpd awqkiqlkea emnkvpemfv
     2641 dttpqqapvf tkhlesydkl vegqhvylea qvepradpnl rvewfkngis lqtgtrlrst
     2701 fdfglvtlsi nglrpddsai ytckatnllg eavstcslki vdrhwllgdt lhpdalpkid
     2761 aleqphvtsa eqpepiyeep vfithlnnve cvegdnvhfe cnvepskdpt mkiewfingk
     2821 plptgarfks tydfgyvald ithayeedsg vvivkatnsk gsaqtsgtlk ctskqsiylq
     2881 tqhpqgeagl ekvkeaeday lskyrrpdeg peheypkpiw tvplqpefkl gefeplhleg
     2941 qvepkddpnl kiewyfngkv lehasrfkmt sdfgfvtldl tdvyerdsgi ytckaynkag
     3001 eaftsttiyc stkeniiers qhpkgkegle aiqdleeslk rqegappese eghppvftsq
     3061 fenltnlseg eiahfeaslt ptgdqtmvve wfyngkvlea shrirtvyaf gmvvlevlgt
     3121 kiedsgtysc ratnkwgqae isvelqcvdk skgqkpkftt hiqsleglkd gqsahfectl
     3181 ipvgdpqmkv ewfhngqplr hssrfkmvsd fgfvvmdiag vmahdtgeyv ckasnkyged
     3241 ftkatlkcfg ksgvyldslq pdslarirel esfggeqptt pttpvaeppk fitqivditr
     3301 lvegqsahfe arltpvndpd lkvewyyngk klphghryrt fhdfgivild ilycyeensg
     3361 vyecrafnky gedttkatlk cfskaslile sqlpkgmegg lekiqtleds mirtkdekvv
     3421 eergkapmft vplsnidglr egesahfear liptddpklk vewfwngkpl rtgsrfrtfc
     3481 dfgfvileis piypedsgey scratneyge avttctmkct gkrsiilesq lpkgmestid
     3541 kiaeleglgn epgqvtpedd tgkppefvtt psdltltens lahfecrltp indssmrvew
     3601 fhngkpllag sriktihdfg fvilevancy qrdsglytck atnrhgeatv ncklqvrgrq
     3661 giilepqlpt nfktgtesiq kleealykrd eilteeetpn ppkftvelkd ieveegapsh
     3721 fdcrvepvgd stmridwfhn grsfatgsrv hqindfgfis ldmsytyard sgeyvcratn
     3781 kwgsattkat itckskktid fdsqlpsgms geklkelerg pvseppveea prqpprfith
     3841 iqsatvdese pvrfecrvep kddpnlriew yrngkmipag hryrtmydmg fvsmdilyvy
     3901 pedsgeyvck aindlgedtt raslsckklp siilqnqvpk gmkksealmq meatikkyts
     3961 evhlteddly dadkkqpprf vtqildqtel vemnsskfec qlapvgdpnm kvewffngkp
     4021 lphknrftpi ydfgyvamnf gwvypedsge ylcratnlyg mdetravirt agkpgiiyes
     4081 qlpkgmksie kiremeaawq ivpeeegeee kvrapptfvs kpepvtveeg dcsrfccrvt
     4141 ghprprvmwi inghtvvngs rykltydgmy hldipktrqy dhgkveviar ssvgesrtet
     4201 tltvkprsdd yrgvlknspr pwydygltqy qterqntele kvfderhhnv sqgieiateh
     4261 lgqkvfkepe tdwqksvksk kneeyynklm tleeeqvlke trlresshqf aipgekvvad
     4321 svakgmaqky eetleekkde ktqetvqppp rplkkphtte vdidvermkv tgkyppgpse
     4381 stvhgrevhv akqkqtqkev kgdleitrki tatetteveh kaktqervvq gptkpakppv
     4441 ftkkiqpcra feqeqarfev efdgdplptv kwyredfpiq nsadlqiytf stksvlivrq
     4501 vfmedsgvfs viaenrggka kcsanlvvee rrrqpgrggv ippsflstiq ntsvttgqla
     4561 rfdakvtgtk pldvywlkng kkitadiryk tleedntytl llletvpeds gkyecvaink
     4621 agearcdaec tvrgpqspak sakpttpgve kapavleplk dqtiregtsv afscrisgkp
     4681 vptvqwkkgd kvikpskyfq mskegdfctl riseafpede gvykciaknp agdvttsanl
     4741 rvlapdaadv lpkltplkdq ivsegqpaqf ktqvtpskpk ptiqwyrega lipqspdfqm
     4801 ihegnnavll iattyeedtg tftcrattsa gtvetsakli vkkrtgayye vpaetgpgar
     4861 tgdkpfvslg tgkdvilepi pldkgeleip lkpgdetlpw rkgrvhhrpr ladttpwrkt
     4921 kpksrdtsld klqatesqik pwteetvtlk ktpqqareip kekleevelk pakiekkeir
     4981 rpsletvdlk pftkettket kieekltrre eidkeiyeee edsrflktsr kvdetvqrkk
     5041 devkpwteek vtlkkskpek kelpketlet velkpskigk gevrkpslek vdlkpvpttv
     5101 tekvsekvte kteyleqedt slldvsrtee vkkkrkekve ekpekpeqak pwteekitlk
     5161 kskpvvkeie ketletvelk pskigkaelr kpslekvdlk pvpttvtekv tekitektey
     5221 leqedtslld vsrteevkkm rkekveekpe qakpwteeki tlkkakhlpk eieketvetv
     5281 elkpskitks eirkssvekv dlktvtketk eitqktdyre eedttllevt kdtqvkktrr
     5341 esipvekveg pkpwteekvt lkkakpekke vpkekpetve lkpsrpqkad iekpsledvd
     5401 lkpipkkvtk ektekdkyie eedtsllevt kdtetttkve kkvkrreeve kkpetkpwta
     5461 ekitlkkakv eqkeirresi eevtlkptkt ekrqvtketl ekvdlqkvtd ktqfveeedt
     5521 tlmkvdkvek veeervdkma twrrdrkpke kapyheeedk sildvkrdee stekrkevpv
     5581 pwargkkkpt ekapyheeed tslldvkkde esiekqkevp vpwargkkkp keekpelvpq
     5641 qvqpeekpee eetlektqvp wlrdkkvark iperkeepve diksvtlkpv rrerapeeke
     5701 kpeevtlkpv kripreeeek eevqlkpipk rkpeekpqee iilkpfqkpk peveeqekpk
     5761 lkpfekakpe vpeqekvqlk pfkrekpevp ekekvelkpf krekpeetlv pqkvpekpee
     5821 eqpseveevs wrrkpkkkea iipekpvekv eevkvvedkv ikkqeetied fksftlkptk
     5881 rgkkleekee keevtlkpvk rlpkeeetka eiklkpvekk veekeakplk pemaptvpee
     5941 ekptevsevp wkrgkkkpve krveevttee kkveeeekev selswrkpqk ekvkevkkeq
     6001 evedftavml kptrkekkpe ekeekeeitl kpiqkvqkee ekpeavtlkp vkvekpeave
     6061 keevpeekte lpwrrgkkpa kkivpqeevk peeigkpeer vpevkeevpk kpkvekpeee
     6121 klevklkpvk rlekpeeakp eevtlkpvkr lpkeeeekee vklkpikrek peeekpevkl
     6181 kpfkrlekpe eekpeevtlk pvkrlpkeee ekeevklkpi drkkpeeekp evklkpikrl
     6241 ekpeeekpee valkpvkrlp keeeekeeik lkpikrekpe eekpevklkp fkrlekpeee
     6301 kpeeitlkpv krrpkeeeek eevklkpikk ekpeeekpei klkpikrlek peeekpeevt
     6361 lkpvkrlpke eeekeevklk pikkekpeee kpevklkpfk rlekpeeekp eeatlkpvkr
     6421 lpkeeeekee vklkpikkek peeekpevkl kpvrrlekpe eekpeevtlk pvkrlpkeee
     6481 dkeevklkpi kkekpeeekp evklkpvkrl pkeeeekeev klkpikkekp eeekpevklk
     6541 pvkrlekpee ekpeevtlkp vkrlpkeeee keevklkpir kekpeeekpe eiklkpvkrl
     6601 dkpeeakpee vtlkpvkrlp keeeereevk lkpvkkekpe eekpeeiklk pvkrlekpee
     6661 tkpeevslkp vpkvvpekde ekpeeeapet silpwrrgkk tkkvvdfhee vtvvpidqkk
     6721 ivsevheket tiveqkveak eviekkvvve evkpevveev tevsevswrk prkpkkpvee
     6781 kevvelkpve kheeivekve ekitlkkvkk evpeveekke iqlkpvpkkp keeevpekit
     6841 lkpvkkekpe ekpkeeeipe lkpvklekpe kveeteekee vmveitrkkp kkkpeevpve
     6901 keevveteit eiteekpeei svlpwrrpkk vtekkievee ikpekvedke veevtettev
     6961 swrkqrkpkk pieeeekpek vtlkpvkrek peekpkeeip elkpiklekp ekveeteeke
     7021 evkvevtrrr pkkkpeeiqr eeievetkev vddvevekie kqeiqiedrk rkrkqrtqvd
     7081 isiidkdkki aprfiqklqp viaepestak ftctvfgspv peiswyrneq elhasekyim
     7141 tvyettasle itkvkeedag myscrasnpa gvatstvnlv ifekeeegia phfstpikpl
     7201 mveehkpall ecvvtgspvp evkwyrgeqe lkpkkgreit fnpetgeakl hilepteede
     7261 tiyrvravnk fgraecranl linnvvkvsk pevlrapkit rplpalvaer gkpltlsadf
     7321 eskptpevkw frngvelaps eknviniyen taelfipevt kkdggkyeir vqnpvgears
     7381 sgsvtvkdrp dktdevkapr fieplqpqiv aegevvimet kvesyptasf qwfhetrple
     7441 stpqvrivtq enksilmike ikseyagtyt craenvggsv tctatinlle tpweetvelv
     7501 sptfvkrlsp vrvmdgesan ltcvvqgkpt pkvewyhdsk pikegkeiti vqdtegvcsl
     7561 aitevfpeda geytcravnp vgeavcttsl iveayeyvpd seiassivat slttgqsgse
     7621 edllspketp lfdtdteesa peiikklpql iptkdgeltr levkvkgkpk pegkwykqgv
     7681 eivssqefqi eefedgtsvl tiaetypddt geivfeahnp lgvsttmtyl svegivgtke
     7741 yrkpewvthm eemqealrat qtvprfiqei tdvyvkegdt avfecsysgn ptpdvvwykn
     7801 dkmlmstqnl kvriydddkk tvltikhate eddgayvcki tsetglatsk aklhvtdvtd
     7861 ekkfyeeeee veeqtievkp kekpqkkklr kakevkekvk tervkvdkke vrkeklvpke
     7921 qpeekkivdi eetqilestd iieekptevs qakkivpiqe pvlteatasl kkiddekpkd
     7981 mvpkpeerak rvieeregiv vvsevttedm apdftveskv draqitsesl qtvtvsevht
     8041 etsvqevrra eakqrkakkt vvteeeifee rpkevvveek pkkkkktekv kvreevtiee
     8101 iverqrenia reveeimeml hakefgpgea plrelatigy lvrqgvsvne ineslyrten
     8161 fpalktpeaq nalvqlvere ghgplitqvl teetttdesv vaatvgfraf mrmielqhvt
     8221 veevlthfap eafrprawev teatevetee rvtervdvvr ktevhfmerr derkavrtqd
     8281 irdikeyedt rqthttlrar kdrkpreetk edrdegvqvv eieeiedteq ekmkkkrkdv
     8341 eeieeeietv ivetdskgrl aqkkkkrddl eekedveeee eiiekvrkel kpkrpkigrd
     8401 eveleeieef dvtedksmps imvnvpsqrh qvvpldrtte qapgkpelek akltvdtita
     8461 ltehivpvqe keisdisrik pierkaslsi spvepystte ttvqastgef adtfkptsye
     8521 atpgiipseg vqvsetlvsd agvtslsvsk qeigrtaevs vtlqeattvy etmvrekevp
     8581 tedfvtpqtv kaedtilpql slsvyevheg ltedkleplk tvatkprvnv taveplvvee
     8641 vraedkpgky ypelivptev atqsvisqrq rvteemhape kegeyipgrl ppsqvaqigi
     8701 sygnetavvq qnvvqesegv fisekktetf eattnvtvle gvsvstvqtq dtetsltiee
     8761 tkkavadlnv iemtpavtve tvpsekekey qpgdkpdtkt aetsisslei gsiqstvvqe
     8821 segvyrpdek ptkalaetsi rpeehvmise iqtadypsdf reelkyvqes gtvsvqltea
     8881 kmiqetlthd reermeemvk peermvdtsy dvnrsvevfq ttsvekeadl rvfempeshr
     8941 gktvpthpvv slevemtqpe dnlgevkkev pssgtakveh vslqetivge tvvaenlapv
     9001 ekdkapetkv aeiamneves lhttmvvvse keteytqvsd vkgayastef ttqvapmlee
     9061 vrshspteey lpdqkptsgi aqpshvples itvavqelae kedlyktdvr pdqktanval
     9121 teltsisipe vmqvitassa etlkketkmk eekgrvevee lsstiisevi snetedvftt
     9181 katpkdqkad fnisgreaae ttqvtamtea edlavkrpee qkgkpqvdel tpltvshvet
     9241 geaekelpta evpkektaqp nifgrdvaet mqvltvtsae elaapkapeg qkgkpqveel
     9301 tplsvsqvvs qeaesalpap eapterkaqp dmtgrdiaet mevltmtsad ilaedvkpte
     9361 qkgmpgleef vplsvsqtvs tekedvlpsp eapttktaqp sltgrdiaet mqvvmmtsek
     9421 elitalapet hkgipnveel tpltvsqtvs heaedvfekt aapsevtakp afsgrevaqt
     9481 semmivtnvg elekqvlpde qkgqaqldel mpltvsqvis geveeglpea ekpkektaqp
     9541 gilgrdiaet tqvltvtsae elaapkapeg qkgkpqveel apltvsqvvs eeaegilpap
     9601 eapterkaqp dmtgrdiaqt mevltmtsvd tftedkkpde qkghpeleef vplsvtqtvs
     9661 nemedvlpsp eapstkiaqp sltgrdvaet mqiltmtsee elitaqtpeq hkgkpnveel
     9721 tpltvsqtvs qeaeeileka aaptevtakp afsgrevaqt seimivtnvg elekqvlpde
     9781 qkgqpqldel tpltisqiis geveeglpea ekpkektaqp gilgrdiaet tqvltvtsae
     9841 eleapkapeg qkgkpqveel apltvsqvvs qeaegilpvp eapterkaqa dltgrdvaem
     9901 mevltmasvd tltedkkpae qkgvpgleef vplsvsqtvs tekeevlpsp eapttkvaea
     9961 sltgrdiaet mqvvtmtsee elisarvpeq hkgifnveel tsltvsqtis heteeileka
    10021 aalgevtakp aftgrevaqt setlivtnvg elekqilpee qkgkprvdem tsitvsqvvs
    10081 geveerlpea ekpkektaqp smlgrdiaet tqvltmtsae elaapkapee qkgkpqveel
    10141 apltvsqvvs qeaegvlpvp eapterqaqp dltgrdvaqi tvvmsmasad vmiddkkpee
    10201 qkgvpgveef vplsvsqtvs tekedvlpsp eapttkmaqp sltgrdiaet mqvvtmtsee
    10261 elitalapet hkgkpnveel tsltvsqtis qetevileks aapseatakp aftgreiaqt
    10321 seilavlnvg elekhvlpee qkgkpkleel tpltvsqiis geaeeglpta evpkektaep
    10381 smlgrdiaet tqvltmasae elmapkapee qkgkpqveel apltisqvvs qeaegvlpvp
    10441 eapterkaqp dltgrdvaqt tvvmsmanvd imieekkpee qrgipgleef vplsvsqtvs
    10501 tekedvlpsp evpttkmaqp sltghdvaet lqvltmvsee qlttkapeeq kgkpnveelt
    10561 sltvsqtvsh eaedilekav apseatakpa flgrevaets qvltvttvge lqkdvlpeeq
    10621 kgkpqleelm pltvsqivsg eaeerlpeae kpkektaeps mlgrdiaett qvltmasaee
    10681 llapkvpeeq kgkpqveela pltvsqvvsq eaegtlpipe aptertaqpd ltgrdvaetm
    10741 evltmasldt liedkkpaaq tgapgleefi plsvsqmvst ekedvlpspe vptekmaqps
    10801 ligrdvaetm qvltmisame lttghapdqq kgvpnveelt sltvsqtvsh eteeilekav
    10861 apstvtakaa lsgrevaets qvltvsnvee feksilpegq kgkprldelt sitvsqvlsn
    10921 eleetlpspe kpsekiaqpq migrevaeqt evltvtnvee lkklekpegq tgkpdveems
    10981 fitvsevfst emekempspe vpkertalpq lssievaett evltmisaee llkskapeeq
    11041 kvqpkldelt pltvshvvse eaeealptae ipkektaqps llgrdvaets qvltmasaee
    11101 llaakvpeeq kgkpqieela pltvsqvvsq eaeealpsae vpvereaqps mtsrdvaqtm
    11161 lvtsmsnvdi maeskipeeq kgvpgleefv slsvsqtvst ekedvlpspe vptekmaqps
    11221 ligrdvaett qiltmisate lttdrapeqq kgvpnveelt sltvsqavsh eteeilekle
    11281 apgtvtakaa lsgrevaets qvltvsnvee feksvlpeeq kgkprldelt sitvsqvvsn
    11341 eleetlpspe kpsekvaqpq migrevaekt evltvsnvee mqklekpegq kgipdveems
    11401 fitvsevvst eaekelpape apkerkalpq lsgievaeks evltmtmtee fakpkapeeq
    11461 kgkpqleell slsvsevalg evekglptpd kpteetakps llgiqvaeks qviassitge
    11521 lqesikpdtk qiipeqipfe sieqsqtltq erestfvvek tttsataevs frvseslevt
    11581 qvtatekesk evikgvakea saqadvierk valktevqpe dmasefkvkk pegktakgvd
    11641 eiqqgvivte hltahefese lpesvlpfak larvsiqadh lehivettea paqqhhitit
    11701 qhttkhdlpq qqsiesdtev ieeyttkfrr gstedataav ktkktivrkk pkttgeediv
    11761 vvieeelddi kpkipsipkk qfmpieevtt vsdveeivsa rideaeeivp keeekvevti
    11821 tevqdtdkkk kvtkkqfvrr rgktrqvtee itveeegkap ettvvtkpve iieedieerp
    11881 kiekeeyvve evpeektipl kprkkltptv qqveeqvtvt eeitkegkpr kttkkkvitr
    11941 kgkeqqvtev vtveeqgkap itsvtegpve evveeiiqkl papkyakpse veevreqvtv
    12001 teevtrlgkp kkttkkkiik rkgkeqqvte vvtveeegka ptttvtegpv eevvieeike
    12061 lpipefvkpt eveevreqvt vteeltkmgk pkktikkkii krkgkeqqvt evvtveeqgk
    12121 apvttvkegp veevveetit pllapeyakp seveevreqv tvtqevtklg kpkkttkkki
    12181 ikrkgkeqqv teivtveeeg kapkttvteg pveeiieeei kpefikprev eevreqvtvt
    12241 qevtregkpk ktikkkiikk kgkeqqvtev vtveeqgkap vttvtegpve evvdetvkal
    12301 papeyakpse veevreqvtv tqevtklgkp kkttkkkiik rkgkeqqvte ivtveeegka
    12361 pkttvtegpv eeiieeeikp efikprevee vreqvtvtqe vtregkpkkt ikkkiikkkg
    12421 keeqvtevvt veeqgkapvt tvnegpveev vdetvkalpa peyaepseve evreqvtvtq
    12481 evtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk ttvtegpvee iieeeikpef
    12541 ikpreveevr eqvtvtqevt regkpkktik kkiikkkgke eqvtevvtve eqgkapvttv
    12601 negpveevvd etvkalpape yaepseveev reqvtvtqev tklgkpkktt kkkiikrkgk
    12661 eqqvteivtv eeegkapktt vtegpveeii eeeikpefik preveevreq vtvtqevtre
    12721 gkpkktikkk iikkkgkeqq vtelvtveeq gkapvttvte gpveevvdet vkalpapeya
    12781 kpseveevre qvtvtqevtk lgkpkkttkk kiikrkgkeq qvteivtvee egkapkttvt
    12841 egpveeiiee eikpefikpr eveevreqvt vtqevtregk pkktikkkii kkkgkeqqvt
    12901 evvtveeqgk apvttvtegp veevvdetvk alpapeyakp seveevreqv tvtqevtklg
    12961 kpkkttkkki ikrkgkeqqv teivtveeeg kapkttvmeg pveevvieei qelpipefvk
    13021 pteveevreq vtvteevtrl gkpkkttkkk iikrkgkeqq vteivtveeq gkapvttvte
    13081 gpveevvdet ikslpapeya kpseveevre qvtvtqevtk lgkpkkttkk kivkrkgkeq
    13141 qvteivtvee egkapkttvt egpveevvee evkpllapef vkpseveevh eqvtvtqevt
    13201 regkpmktik kkiikkkgka qqvtevvtve eegkvpvttv kegpveevvd etvkalpapk
    13261 yakpseveev reqvtvtqei tklgkpkktt krkvikrkgk eqqvtevvtv eeegkapett
    13321 vtegpveevl eeeikplpgp efvkpsevee vreqvtvtee vtregkpkkt ikkkiikkkg
    13381 keqqvteivt veeqgkapvt svtegpveev vdetikqlpa pkyakpseve evreqvtvtq
    13441 emtklgkpkk ttkkkiikrk gkeqqvteiv tveeegkapk ttmtegpvee vveeeikplp
    13501 alefvkpsev eevreqvtvt eevtregkpk ktikkkiikk kgkeqqitev vtveeqgkap
    13561 vttvtkapve eivdetikel palefakpse veevreqvtv tkevtkegkp kktikrkvik
    13621 kkgkeqqvte ivtveeegka pkttitegpv eevveeeikp lpalefvkps eveevreqvt
    13681 vteevtregk pkktikkkii kkkgkeqqvt evvtveeqgk apvttvqegp veevidetik
    13741 slpapeyakp seveeireqv tvteeltklg kpkkttkkki tkrkgpeqqi teivtveeeg
    13801 kapvttvteg pveevvkevl kplpvpkvvk pseveeireq vtvteemtke gkpkkiikkk
    13861 vikrkgreqt vqevvtveek gkapvttvtv gpteeiveet vktlpapeya kpseveevre
    13921 qvtvtqevtk egkpkkttkk kvikrkgkeq qvtevvtvee qgkapvttvt egpveevvee
    13981 llkpliapey vkpseveevr eqvtvteelt kegkpkkvik kkvikrrgre qqvkevvtve
    14041 eqgkapvttv tegpteeive etikalpape dakpseveev reqvtvtqev tkegkpkkti
    14101 kkkvtkrkgk eqqvtevvtv eekgkapvtt vtegpveeiv eetvkalpap elvkptevte
    14161 vpeeltvtev vtkegktkki vkkkvpkpkk egieapeeap eevpveetkk iiklqrlkvs
    14221 kfestkldiq etslgkpqfa qiklrktpva krpskkdekf prillrsrii aiewppspkn
    14281 lkvdtfepie lqngilsrnv deaaalpkpk kkkikrieke iaklekldqe feelkkelpl
    14341 ekleepilee kperkvkkkk kpedkakegv kpqlmrisik eqkpikpkia epatplfaqi
    14401 klkkapakpk kkpeeevskf pkillrsrit ahewppsvky paisvlepny iqngilsrtm
    14461 eealklkkvk hkkmklpeke ltelekldqe feelkkvple kveeitpyer kpkpekpeee
    14521 kpkklvigkg krrpaeeedl etvqlkkipe kipsapeeae kvpkkkdevd kpkkekeeke
    14581 ypelkpfepy dieapevele erekpeypep ekpkekkvpg kkpkkerktp aqeteavpiv
    14641 pgvpkpkepe eeeeikrrip erpppteepe kiklkpwkkg ekpeeeekkp edyikfvpkd
    14701 ddivetvevt ttveetpdkk qrkikkkkti tkpkdekpki ieettieeeg fepvvtveef
    14761 dvdkyeavpv esvekaapve eikeeivtse iiteegtkkt vrkkrvtkkr egkervteev
    14821 tveeegkepi tatielhedt iseiheqakh krptkpkade klaedvtekt veqqeiieev
    14881 kevkptkkvi kkekpvkakd evteevveei iieepekqea kpiveeeetv iitehdvrka
    14941 pkkkkevvik eeeeifetie tpthkiirkk irpmkkgeke vvveeiiqkp reetvemeeq
    15001 qivevvetpt kkivkkkkev vkddgvpeel veeiiiekpq kkkakptvee ekkkvkvtkv
    15061 dtkkapekpe evveeeeeii etietptkki irkkpkdkkd kiieeviqkt ieeakameeq
    15121 eivevietpt kkvikkkttt ieegkapeek veeiviekpk evieeeeeii etietptkri
    15181 vrkkpkdkkd kiveeviqkt ieeaktmkeq eiievietpt ktvikkkkat ikegkapeee
    15241 veeiviekpi kekakkkilp kegveitevi tetsveelie eelkpeeakp seeveeekvk
    15301 itevaikkvp kkkkpskave aeekeevvee ikpekpkeek akptvtekeg ievtevisev
    15361 apeklpeeak pkkkkateik eteekvitkk vkvkeapkea vpgeeeqpae evdelvpekp
    15421 kktkakkevt pqdsivttev vpitkeeele vekpeeekat vveekeekak veekkvtvkk
    15481 apkkklkpte aeeeqveevv eeitpeepkk rkakkavipk eeiqttevip gitaeeipee
    15541 ekpeeekavp keeekveeea kvtevsvkkk erkkkekpvt ngevieekpk vkeipeekpk
    15601 eevpqvpeek ekpeeekpke vkpkekkpke ekpkkpieek peevkpeeek pevkpeeekp
    15661 keekpkkkkp aekkpkisee erpedkveei pekkpeevkt reeiipkdtv qttevipetv
    15721 aeeiptkkpk erkavpveek eekveitevp vekapekkee kvpeekptea apekpkeakv
    15781 mkkkkkkpak kdeieewvep eyerpvlepm pekivwepkk kkekkplpes lqklvpqkie
    15841 rkemkptklk ysepaetvqf asiklkkvav pkkkeadetk fpkimlrsrm tfvgdyppel
    15901 qipkitylee npvqtgilsr nteealkvlk rkvkkvklse kevtqlekld lefeelkkvp
    15961 lekiedksvy erqpkkpaek peepkkvpig kgkvpeeekv epekvklkkv pekkpeevee
    16021 plkkpkkpep eeevkpekkk kpkvklehde lkpldfdrpe lekyepeeye kpeepapepv
    16081 pkpyekepkk kpdqeveqip lkkgkpkspe pdeepevkfr iptkekpeee peqitlkpwk
    16141 kekpeeekek eipkkeeekv pkppkeeped vtvqrkaktk kpkekpkeek eeevtvkkpe
    16201 eekpeevaee vtlkkepekp veeapaevti kkpvveekev eeeetitevi lkkkpekpee
    16261 vveeavvkkp kkkkpvkeee vveevtvtkv vteeekeeek vvikkpkkks ipeiteevte
    16321 eitlkkpeek apeevteeik kpkkkkpvke evaeeitikk pvveekkeee vpeevtlkpk
    16381 kpeekpviee vteeitlkkp vpeevteeva ikkpkkkkpv eeeaadeitl kkpvvvekee
    16441 eeiteevtlq kkkkpekkpv peeiseeitl kkpvpkeeek apeevteeia ikkpkkkkpv
    16501 keeaadevti kkpvveekke eetpeevtlk prkpkekpvp eevkeevtlk kpvpeekekv
    16561 peevteeiai kkpkkkkpve eeaadevtik kpvvvekeee eiteeitlgk kkkpekkpvp
    16621 eeiteeitlk kpvpkeeeka peevtekiev kkpkkkkpve eeaadevtik kpvvvekeee
    16681 eapaeitlkr rpkekpveei teevteeiti kkptpeekek apeevsekve vkkpkkkkpv
    16741 veeaadevti kkvvveekke eeapeevtlk prpkkksvte eevaevtltk pkpieeekkp
    16801 eeseltikkk pkkkpvieee aaevtikkpv pvepdeapee itirkkkkpe kkpkvteeve
    16861 etavtikkar apekpteeee ittdvqikkk kpqkkveeva adeltikkie eveqpeeeee
    16921 vqeftfkrkp pklppkpiee iyedvtlrkl rpkrksrpdi keitetenvt frprttktke
    16981 dveqefkisl ntyeeedism sgkvrlkpkk kpltyseetg eetiriiqev eddsgpiiee
    17041 iiesdeegkd eysieeldtd emrlplrrkk kkvpkpykve dieegdvklk lkrerkysie
    17101 eadeslalkl kskrrvstyd eeeatlsitr eediseevei eyvvrdgdtm fsicsyvaet
    17161 deainlvege rvyvidhtnq dwwfvkkhlt eekgwvpaqy llnevhytly lqrklhekid
    17221 klpvfekpap gdkssapkfv eklqpihtpd gytiqfecqv egvprpqitw frqtaiikps
    17281 pdfqmyyddd nvatliirev fpedagtftc vaknaagfas stteliveap lsdhgsdltg
    17341 psrkslsres sladilegip ptfsrkpkak yvdegedvil ecrlvavpep eitwyykdtq
    17401 ittkeniiia tesdmhmycs vikitkvqkk qegkytivar nregeatiei pmkvktgkse
    17461 ppeileplqs yvvregetvv lstqivgnpa pkvtwfkngk plkgltpkqd ghvntltliq
    17521 pqvsdtgeys vvatndlgta etkatltvek ipsgapeppl fterfqeltv pekgtfklva
    17581 kvtgnpvpev twlrnnkple kspnitetyd genivleirn ansevdtgdy kcvasnpvgk
    17641 ashgarvtvd vdkvtftktl ekevvvdeyk tleltcetsh tvstkwwhnd keisgmdhre
    17701 iieegrvhkl vikktnptde gtykctvknq stssnvtvka tkpefvkklq dcevkerdva
    17761 ileveitsqt vdvkwykdge qlgpgkekle fvkegtvrkl lirstsvhde geytcslldq
    17821 ectaevtvie lppeiitkmq dvtiargera tfdieltkgd alvrwfkdgq elqfsehvrl
    17881 sidgkrqklk iydtesedag vyscevgqqk ssakltveep avefitklpd vtlvplnsda
    17941 vfvielsqdv pvtwmrktqv ikespkytii degkvkkliv kkcttedise ytamvanvkt
    18001 ssrlrvevie tppkispdtp rkykvrkded vdivvkfsgt pkpedewtvn grvvtkskra
    18061 iptideesav ltirkiqeed vgdytlklvn svgetsiein vvivqvpsap gapeplettn
    18121 dsvtlhwkkp dsdgnspive yilehqekte ttwtkiteti tetthkvtkl ttnkeytfrv
    18181 tavneagpge tsptspyvki skpstaeppv ileplksivi glgetvtlsc viggtpapei
    18241 twlrndeife dtsityenhi tkytirette tssakftvka knnvgsaett celkiqespk
    18301 iiydenltnq klpmngqwki eiqitgfpkp evtwtkngke vdekrvsihr eertstvsis
    18361 slvredtaty makaenqags ssvdlhlkvi dkpskpqgpv vfkeirqdrv tlewrppadd
    18421 ggielekyti ekcepgktwv kvvdidkeve sfcvhklqqn aeykfriiar navgaseple
    18481 sdtvkmrtsf eppgaprgpl evsgmtktsf tikwqppend ggtpitdyii eikeeskktw
    18541 qkigstkhet thfsvsdlkt dvpynfrita knsvgsgppy vaeepiapgr ritppsspqh
    18601 vhvtnitsks atlswsppas tggteltgyv iekrpligkg arwtkvvtld attlqycven
    18661 lkeseflfri faennqglsv pansepvtlk thasvpsppt apleirqiaa ntvviswgrp
    18721 dsdggapleg ykvairdtkr tmwmevarvn teiqklnird lqenhmylir ifarneigls
    18781 dpleseepfk iipaseltvv epiaeatekg etasisfste ntsswlrehn mdadihsyar
    18841 arllrqdeyf friwhyakkl fe
//
LOCUS       XP_034183027            9587 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605608 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183027
VERSION     XP_034183027.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327136.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183027.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..9587
                     /product="uncharacterized protein LOC117605608 isoform X1"
                     /calculated_mol_wt=1089640
     Region          24..111
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          122..211
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          139..143
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          152..156
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          177..181
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          191..196
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          204..207
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          220..299
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          236..240
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          249..253
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          265..269
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          279..284
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          292..295
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          322..399
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          339..343
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          353..357
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          379..383
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          393..398
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          423..498
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          439..443
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          452..456
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          468..472
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          478..483
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          491..494
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          520..609
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          537..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          550..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          576..580
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          590..595
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          603..606
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          619..696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          635..639
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          648..652
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          664..668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          678..683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          691..694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <2174..>2305
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <2593..3231
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2931..3801
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          3812..4597
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          4412..5192
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          6830..6920
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6847..6851
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6860..6864
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6886..6890
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6900..6905
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6913..6916
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..7007
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          7047..7133
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7064..7068
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7077..7081
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7103..7107
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7117..7122
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7130..7133
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7160..7240
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7168..7172
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7181..7185
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7206..7210
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7220..7225
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7233..7236
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7282..7371
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(7282,7350,7365)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(7366..7367,7369..7370)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          7392..7473
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          7403..7407
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7416..7420
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7439..7443
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7453..7458
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7466..7469
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7500..7590
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7517..7521
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7530..7534
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7556..7560
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7570..7575
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7583..7586
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7600..7691
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7618..7622
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7631..7635
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7657..7661
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7676
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7684..7687
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7722..7814
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7739..7743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7752..7756
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7780..7784
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7794..7799
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7807..7812
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7839..7929
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7856..7860
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7870..7874
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7895..7899
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7909..7914
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7999..8088
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8016..8020
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8029..8033
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8054..8058
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8068..8073
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8081..8084
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8112..8201
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8142..8146
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8167..8171
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8181..8186
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8215..8301
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8233..8237
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8246..8250
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8272..8276
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8286..8291
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8317..8407
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8334..8338
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8347..8351
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8372..8376
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8386..8391
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8401..8404
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8624..8704
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8640..8644
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8653..8657
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8678..8682
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8692..8697
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8716..8812
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(8716,8786,8801)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(8802..8803,8805..8806)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          8856..9105
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(8856..8860,8862,8864,8877,8879,8908,8924..8925,8927,
                     8931,8933,8971,8973,8975..8976,8978,8990..8991,8994,
                     9009..9012)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(8856..8860,8862,8864,8877,8879,8908,8924..8925,8927,
                     8931,8975..8976,8978,8990..8991)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(8860,8931,8933,8971,8973,8975,8994,9009..9012)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(8990..8998,9001..9012)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..9587
                     /gene="LOC117605608"
                     /coded_by="XM_034327136.2:210..28973"
                     /db_xref="GeneID:117605608"
ORIGIN      
        1 mtltdqtaka kgpprwldpd qvlsvqgrtg edvvfepklr wsdvfdelrf eliredvpip
       61 sdryrvqmrg nvvqltlkqs qkddtghyal vatrlgqgfd kgsskkihls vdepiseegd
      121 ppvflrrltd lavkvgtrtr flveirsstt pkitwhrnae pvhagsrfsf vhegnfycvd
      181 vapvtvedeg qwtcmaenps grssctsrlt vivpkaykrp efveelrall tetgtvslec
      241 kvvgvptpvl rwfkddkeik agdvfaltan pddptslgiy tceavncmgi syssskvhvv
      301 gkgsregslk padsmtpsgp lpvfsrilqd eccrigdtlr lscqvqvppw pktitwynke
      361 grieatekyh vmedglggys ievnpveamd egewkcvass tedmkqfttc yvamsipkny
      421 rkprfmeslk avlteeglvs feckvvgfpt pllrwfkdgq elkpgdvyql tgtnslgsyc
      481 ciarncmgea kstaeltied iqnqlneeer mqllstnqpp sfikglrsce arineefhft
      541 vqvsispvps lawyrddvpv eenekyqiak enlgtchldv rklefvdqae wkcvasndfg
      601 hsvtscflkl iipkhykkpk fleslrails degavnleck vigvpqpilk wykdgvelkp
      661 gdihriisgq dgtcclgtyt ceatncmgtv sssasllgfe dkfpvrkeak evqspnghel
      721 arnlslstih eertsqlydt pqtdhsvtld drgevsfsfd gkevsvslye tpdlteeeal
      781 qivemyadql sehvtehnvi elppmrfvke ssnsgnllme avvidvspdy fvsaedgddl
      841 rteadfedas ilddvtrvfs sperdsrgsl krsamysled ddkppsrppr kkstssskse
      901 kseksqkmes esfhsaqkde pfspvspmsp vsslkqddsd tfadalssar lsisenqvqk
      961 lyeaghdrkr slsadktags siddgiggds sfdsvtgvpk kkkhrkkkhk kekgsseess
     1021 igseidgkrr rsrsasksdl hqriesinkt eepyvedvpd ksserkpssd sdkslsksnk
     1081 erlldnmrkm kdpvlvvrdt lvnidqldak lnseseedck vivteniikp iqnlceevss
     1141 veakalksag drsltqnvri slleaiggpt eellrgmeli grqenvknrk tdviailesl
     1201 tdpvdeilmg itkieheltg rakgerpial irmtsavskl denirkvltv dkratrlvss
     1261 ledilstldf flndvsvdps kgtietvdtv vveslsrsve dvaralggfs snvqvskvid
     1321 elltptrelk akldtlksal etyetkgils ehrtkliesl qesvgqsiqe iqkvkekstt
     1381 eeqkapadqa kqdilkevrl tlhealksye niagtldprl stvfdrvdem yrrlrtpqkr
     1441 digietlmnl egplyslqca ltsmaivens emvfkllepa ifqlkltits leesslkpvm
     1501 emlqsiektf sgttneyeet issrsvqdep qtltdrildp lidvqsalsa alqtiedvds
     1561 vkngatspqk ilasseiaac lielrqcvsd tahtamtlre detlnslidl keplldlhla
     1621 ltsedrsfqe lpvirkasvp leklnvvvst vlrhcdsqes ikavsevskm leeiekqlpk
     1681 tvtrlidaee akrhylegmn idenlsnvhf alssvlerqe knypsrvssf tncieelrqn
     1741 lgssavaian lknpfdeeiv eqvyrlketl lnlqrslltq ehdveeeqil kdlispiekl
     1801 knivqsivds daksdavlpv lelleeiekd tpliakevsk kmaqkeaekl ipkkkkqktp
     1861 tfeladkisr sldpmkhwls ttseesiked eesglsstvs elkkdvtkia ietsyseppt
     1921 desliealid lqeplmrlrn aismyhepad lntleglgrp mkyllqtimd vlrdhaeeea
     1981 lrpivdivee ienqipisik ealykkelke mvksmtreet gattsqetiq eeivpqelpq
     2041 evqvhtsqet tadgtlesst vlpeaqfses vieqttieei ssqksdqvit mieevpkqkv
     2101 avdeakkkge eeaiqlvlsl sktlenvqmd vmgiledfeq pttgtttlpi shlansleel
     2161 rktifivgak tgdyaksvhs yeetveetis ilkslqqpln nlqevlresh qhgarellil
     2221 nhliqplnai eeeiinrsie klnhdsklie ncqpvlhlln dikqsvpvvv kdiysrqell
     2281 eslrgilkpl knieermkil enetegtler dvakilvnpl eylleaisvt srelqfldqr
     2341 reivieirnl veplveflsc lsvvqssrks lvpeaallde rrnvilkave dlqrqttiil
     2401 eripnlegas lfekplislk katasvqkqi gktdyskrps svdftlqynl acsfdnlrst
     2461 istveenade svrqvisksl ealqkqials qthfiqtseq pvdeeavveg flyptnqlqs
     2521 alnvlkeqid qktiasvsie svrllqalan stsdlassls mhrvqlirng asegsslvet
     2581 lsavvdvlqs vkdsikaiek vviekegagk vqevenrsvs mdmpmdmpit itqietivde
     2641 vieiskeeie svltierlsp drdqqrteev ksdkmkaitm edesvkkedd tkdksvemmd
     2701 krkeemvepv ekaeissekm edkkekleev eememltekv eelakkqtde eakisekveq
     2761 lgeqeekake veisvekvde ltkkeadqvm kipevaekle vkeekmeaqe kevetekvel
     2821 akketdekaq qlekqnetve eqikkeekkv kvsteemeel stketeskve kleekkkqie
     2881 eeqkvkilse kvdeltqkkt dekvekleek vekveeqvke tekgeaaatk eeelvtkead
     2941 ektkkleeev kmssekqevs vkkdekveek eedtikilse kteelavkee nvqalaeeae
     3001 kltkaeekie vspegtekal ikeektevli gkaeeaikpe ksevssteks ekllqkeekv
     3061 etliekkeei getsktekhk aeltkevvaa ekkeekikgt ekleepsnqe atagtsekpe
     3121 kltkieekvg eaekkekiie kseeaikkee tqalpskvee likkeeitqt ssskaeestk
     3181 eegktveiqr ttnsiitvqq plkeltnlvs saleeplssk seeekrkire ltaliqilyd
     3241 lratvssvqn saspssipel eehllhllns lrtldrstek ilritekelt palkenvmvs
     3301 lqlllepldv lhrefsslqe tavqssayvs lsinvrnvte tisqlmeair ktselkrvki
     3361 veeirvlaam kkeesqdiee ttakpleeli eaimvsqehm kpdaspfgsv spsveptesl
     3421 eqqkleeemm leanlvekiv spvknlrelv akiekqklee aetldlpakq ttsilntilh
     3481 pleelersln issvqqivqt eetvketkqs vqssqsvpvg avleelkrsi atiqeevtla
     3541 svkeasktes gvlfikevek slenlkfsla tvqkvvatdt esveelttqq rtmavetfvk
     3601 svtdlgekcl aivsrpktkv dtaeglqkeq leevlemmvq piqvlrests eietqraeei
     3661 qhmdqternv iealkplvhp leqleqllct avhqdssikt etaeemketg ssveklkltp
     3721 vledlqksia vveqqmalqa kevdtssvkv essvaelies plknlkstma aiqnleteet
     3781 eemtseertt alkafarcle eigqvcsias sqqkmettll qqpqvaklef eqqvleaisg
     3841 piqvlretvs kideetqeae allvsetkea anvlhtlvqp leqlekclsv aveqtsiage
     3901 esakemkdlp asmklnvepv lqelqksiat iqeqvsfetg meksadklet svakaieepl
     3961 eklkasvaav eeivckteqt qnlsesekss ilksfaksve eisqhcvami sqkavpktle
     4021 ksqvaileea isplkalrea idkiedenlq eaekldtlea kelekslkil veplqklets
     4081 llstveqavl tqleeteemk dsvpsaekld ispvveelqk cvatvqeqvk lqtataspee
     4141 kavlvqaaek sleelssslt viqsivatvs neeekvsdmd kmtavqqfak svheleekcl
     4201 vsqeqvevke takeiegida kametivapv halretilki qeenleevgt lrteemklqv
     4261 lsplvhplee lerclitavq qesgqklett eeikkeslek vvlkpileel kqnivivqkh
     4321 lamepessfk leavknitea ieelyttata vqqvvaisse eaeemktska salesfaksm
     4381 eqleerclav inqqkadkie kpekqqgklv daqvlqkitd tihvlresls qieeeklqea
     4441 kelkitkeee eaglklnvli eplnvlekal mtaveeskiv teettqelkk teiaveklnl
     4501 qpvleelqks iavvqqgtsk tavsvdesdl mkmaekslee mklsmaavqe lvshqaqeik
     4561 aeekleafak smeeagenll avakqqelkk atlrieeppa lkvsedfvkt vvtpiaelht
     4621 tivkidketk lskekekeea viflsmvqpl kkleesflsa vqeeikqeaa eeirpaipvl
     4681 qklpiqstlk elnknvaeiq eqlvlaketl trtgstedva viktlersls nlrtssaivq
     4741 qlaaieepgq qildvenvsa lqafaiavee fkkscaaiis rpavmssiig kvhleeitkk
     4801 easstetilv pvkilqesic tieelklqet gildtseikk pttvlselia plqqleqsfm
     4861 aaiqgehvie haaesfetdk asfektalkp ileefqksia ivqehmilee gaqslsdaee
     4921 aslkmmaqtv tdlktsvaai qqitetvpem anelskgesv aafetfaksl hdlveraati
     4981 dhqqiilepa adtisedass lktwadived spvqalepmi vdqgtiesps eialsiseee
     5041 aqvlkglakp lselreclav vveerksmda nemafamser edlsllktva qpllelknaa
     5101 vsvileqtav esageksfee kpetnlnpli esleelchsi aliedqmlve saedrtidev
     5161 slltalaepl mtlqrsvcvl eervlspdve plpeesnwin eclavplqei eksiaeirec
     5221 cvvepeeqsr mvtldwsvve klvepvevir stiigmedtv aeemkkctim knliqplsev
     5281 hesfmllkdr tdltvaeeea sidaivdsls nleksisfie eqtaekvedv sssakddrlv
     5341 igtlteslmq lkesimtvee sptvylenle qplkelqsal esvsnirrek aekektkkiv
     5401 sleeatrvst rlknsikein dsiesidnki esfkgllgie vqienealks lakpleglkg
     5461 giekilekee vegstvdslr klrnaigiir qqsadkplae sfhfetvdiv gmlrpltpcl
     5521 heletctekv etawkensag eglveletpi snlmdtinil rekllrldiv wvteeekkkk
     5581 earkkeeeka eklkqeeeeh kkkeeaeklk qeeeerkkka eaerlqreee erkkkeeaer
     5641 lkqeeeerkk keeterlkqe eeerqrkeet erlkqeeekr ekkeepeklk qeaeehrkke
     5701 eaerlkqeee ckkkeeaekl kqaeeegikr eeeerkqeee klrqegeark kkeeeekikq
     5761 eeetrkikek eeklkqeeee rkkkedeekl rqeeearkrk eerlkleeer kkkeegeklq
     5821 leqerkkkee edklkqaeee rkkkeeeerq wkekeeklkq eeetlkqeee arkkkeeqer
     5881 lkqqeekrkk keeednlkre eeerknkeka eklkqkeekl kreeeerkkk eeqdrlkqqe
     5941 eerkkkeeee klkleeqerk kreeaeklkq eeelkkkqea eklkqeeerk kkeeaeklka
     6001 eeerkkkeea eklkqeeerk kkeeaeklka eeerkkkeea eklkqeeerk kkeeaeklka
     6061 eeerkkkeea eklkqeeerk kkqeaeklka eeerkkkeea eklkqkeerk kkeeaeklka
     6121 eeerekkeea eklkqeeerk kkeeaeklka ekelkkeeea eklkqeeerk kkeeaeklka
     6181 eeerkkkeea eklkqeeerk kkeeaeklka ekelkkeeea eklkqeeerk kkeeaeklka
     6241 eeerkkkeea eklkqeeerk kkeeaeklka eeelkkkdea vkqkedkkhk kkeaeklete
     6301 ekhkrkeeae kqkeeedrkk keaeqlkeqe erkkreeaek lkqeqenrkl eeeskkkkee
     6361 qerkqkeqae klkqeeaeis kkaesdtlkk keeklkrkke elkqqreede rkekeeaekr
     6421 rkeqeqrkke reerakkeee erlkreeeer krkeekakqk keeenrrkae eaerlkkeqe
     6481 keeqrreetk rrreeqekqi rqetekirka eeerlkkede trerrrkerd lrrqeeeekl
     6541 hkeeeertkr eeerrrrrre aerqwredee airrketerl errraedrqn reemerlkre
     6601 deerrrrrde drqtrheetv kalkeehdrl rkrqeeetsr lrkrrqeqir ddswsrmrqs
     6661 esdqllkkmt ddsfrlgtsk tfssdldfhr dksykydsgf esalssasrs yswrdsltsl
     6721 trrrvddfid yklrdtsmdr yyfdtggyyr rrrkrddrit rarsisllky ddystgdsdt
     6781 titpaspils rysrtktrsr ndlddrgsnl slylppikde itprekgkkp sfctrltnrt
     6841 vgvgmrtrlt ctvlghpepr vywtkdgekl etisnkyrir fdngmayfel healpedsgl
     6901 ytcvaenvhg iastesalkv ypdfqpalsp ptftrsikdt yrpsdnelil ecrvrghpip
     6961 liswlkdgci lqgdrykqcy ldddiyrlei aapnskdngr ytcramndlr teeishvvqf
     7021 nerdrrvtsk heklyddhfs ieafrrprfs nyltdysvpt ggtvalqvev kgvpapevrw
     7081 fcgekkepvs ipkaktftes gvhtlvlpev tesergtymc rainayghvd siaivdvisp
     7141 sgsdsgkpam fvsrpvdksi tvtsgedvsv sfrfsgvpkp kiiwmkglkd itdgprshke
     7201 viddyvrltl krvvpsdegt ycillknryg cdrsffslkv kqrarsltpd wstlssrtet
     7261 gslhgisses rddemsyvkn vpgpissepv vvdggknwls lswgkaerrg papviayrvd
     7321 awllgsdgga rwvelgmtpi nafdafnlkp ggeykfqvtp rnrygwgesv tmtnsvrvse
     7381 tvdlpeftki lpgqlkileg ttvkleceir vdshvdvkwy resteidpry sifyngsrcs
     7441 ltiekvqesd ngryvceasn lvgkassfar vlvvddpkii eadaklksrl lgddtedrpp
     7501 eftmrirdrr vqatypvrlt cqvtgqpvpv vtwykndtei rqderhifwd ddsnfhtlei
     7561 thstledsgc ymvtaknang svscrcilvv dkgirayiap eflcgldaay tvklggdlrm
     7621 taqieaypsv gvvwhrdgnr lrpsrrtvmt lnhdgtvels lakvtardag vycctatnev
     7681 ghaetstrvs iigtetegeq tsldgvptvt lassdipysk eplfvtkpls teaiegdavi
     7741 iscevvgdpk pevmwlrdfl kpdyyrdaaq frlvgegpqy rleipyakld ftgtysviar
     7801 nchgeakavi slqiyakgqg keekmkksgv thgnvltlpv itrelrdlrc cdgdavtlec
     7861 kvqatpeapl vrwerggkil qisgdfssef dgetarlsiq hvypedegey tcvayndlgk
     7921 aftsaclivd vpegkenvlc qrltrpagll sagstprstp rstpirslsp avshgrelrs
     7981 pqllprsrsm lrrpkisppk fyavphnrvv eegetvrfqc avvghptpwv kwdknglavt
     8041 pttrisiker ddvkileive vtqedaglyr viaendygri easarleiin rqllgpvsrt
     8101 irtrsasprt ypsfgrslld ttsringrly ldcsirgtps ptptwfrngr plersnrikr
     8161 yfdgktakve iskvkasdag eytcvatnvl gstkntcqvi vldphdpsts dknapkflqs
     8221 lpdesivmeg hcyelqtrlt gtppfsvtwl kndreipdnd cckyiiygdg gialrlsnvc
     8281 sedageytcl vrnnfgeasc nglfavqdyk gvpklapqft ktplsvitsk getacfcarv
     8341 qcgkpmeiaw tingkdvken prckieqddn vsilriqdvs ardvgdirct asvigkgpsi
     8401 sctaqlqlnr pvrniedsas kcnnaqaeir ngrpppkpgn ftrnnslrkt ardvpslsqn
     8461 qrnespmrsr ssslplrtsp qrsspaptrr ntpsplpdtr kasenklmkk ernfkkleri
     8521 qkdekvplss kdeeatnkpp ddkprlknse ddeekcsmsl nsdfskteng rkrsqdngnk
     8581 hddtpiicme caveepdeas kkdqetvtka ekvdsvrkef laasiikvpa dvtvftgnrv
     8641 vlrvtyrghp eprvrwfrag reltpnkkta itygggvscl isddvtadna gkyevsvene
     8701 fgkdrrcfsv avegppdppa gipsvccspg tvtinwrssp ydggctvtgy tvemshpgen
     8761 twtriaescl slsltapasg tnapipgeky rfrvraenih gvsepgdese fvripkegem
     8821 clqddeeefe aafearlvem edgqlfnnry evleelgkgr ygvvkrvver ssstsfaakf
     8881 vrtikskdke qvreeikimn mlrhpkllll aaayesprei vmvteyisgg elfervvadd
     8941 ftlterdsil fmrqicegve ymhqnnvvhl dlkpenimcr trtshqikli dfglaqtlkp
     9001 dtpirvlfgt pefippeiis yepigtesdm wsvgvicyvl ltglspfmgd ndaetfanit
     9061 radydledqa fdaisndakd fisgllvkrk elrmsarqcl ehpwmaqhae amsrialpte
     9121 klkkfivrrk wqktgnaira lgrmailsan srrsptgtae ssptserpnp idsprpinfd
     9181 vsptteskkh diddrtekkk stgesdvqer nvysfcykte vnilpeetld ssereearvs
     9241 sdldeaslkn epawdrvrsc srgngseeiq etdtseeaqk pdeahleepv pmekieeete
     9301 eiitrcryee ritikeetlf vqnfepernt lniesystsm tscneedysn niseevtssi
     9361 nketdvpean kvktirkiee krideeiset ekvskekidq klrrvfrgds rdsgigdcis
     9421 nlssssqqvn elgissikee etdndnnngn arsvskkveq fenrkifvek mtdnemtqen
     9481 sdvsdpkkct kgsievdtls dvtkinrqhe avneiedprl ligrvrskfv ptgnvsrtak
     9541 lfekeatask spanvpqvpq rtypavpvva gkpqneriqk afafwnk
//
LOCUS       XP_034183034            9562 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605608 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183034
VERSION     XP_034183034.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183034.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..9562
                     /product="uncharacterized protein LOC117605608 isoform X2"
                     /calculated_mol_wt=1086276
     Region          97..186
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          114..118
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          127..131
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          152..156
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          166..171
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          179..182
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          195..274
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          211..215
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          224..228
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          240..244
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          254..259
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          267..270
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..374
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          314..318
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          328..332
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          354..358
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          368..373
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          398..473
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          414..418
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          427..431
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          443..447
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          453..458
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          466..469
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          495..584
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          512..516
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          525..529
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          551..555
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          565..570
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          578..581
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          594..671
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          610..614
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          623..627
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          639..643
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          653..658
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          666..669
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <2149..>2280
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <2568..3206
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2906..3776
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          3787..4572
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          4387..5167
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          6805..6895
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6822..6826
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6835..6839
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6861..6865
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6875..6880
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6888..6891
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6905..6982
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          7022..7108
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7039..7043
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7052..7056
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7078..7082
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7092..7097
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7105..7108
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7135..7215
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7143..7147
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7156..7160
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7181..7185
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7195..7200
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7208..7211
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7257..7346
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(7257,7325,7340)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(7341..7342,7344..7345)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          7367..7448
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          7378..7382
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7391..7395
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7414..7418
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7428..7433
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7441..7444
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7475..7565
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7492..7496
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7505..7509
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7531..7535
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7545..7550
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7558..7561
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7575..7666
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7593..7597
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7606..7610
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7632..7636
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7646..7651
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7659..7662
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7697..7789
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7714..7718
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7727..7731
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7755..7759
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7769..7774
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7782..7787
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7814..7904
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7831..7835
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7845..7849
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7870..7874
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7884..7889
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7974..8063
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7991..7995
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8004..8008
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8029..8033
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8043..8048
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8056..8059
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8087..8176
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8117..8121
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8142..8146
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8156..8161
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8190..8276
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8208..8212
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8221..8225
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8247..8251
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8261..8266
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8292..8382
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8309..8313
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8322..8326
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8347..8351
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8361..8366
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8376..8379
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8599..8679
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8615..8619
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8628..8632
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8653..8657
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8667..8672
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8691..8787
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(8691,8761,8776)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(8777..8778,8780..8781)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          8831..9080
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(8831..8835,8837,8839,8852,8854,8883,8899..8900,8902,
                     8906,8908,8946,8948,8950..8951,8953,8965..8966,8969,
                     8984..8987)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(8831..8835,8837,8839,8852,8854,8883,8899..8900,8902,
                     8906,8950..8951,8953,8965..8966)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(8835,8906,8908,8946,8948,8950,8969,8984..8987)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(8965..8973,8976..8987)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..9562
                     /gene="LOC117605608"
                     /coded_by="XM_034327143.2:715..29403"
                     /db_xref="GeneID:117605608"
ORIGIN      
        1 mgsdcckcan gnkpaikgsf sdgsgygire dvpipsdryr vqmrgnvvql tlkqsqkddt
       61 ghyalvatrl gqgfdkgssk kihlsvdepi seegdppvfl rrltdlavkv gtrtrflvei
      121 rssttpkitw hrnaepvhag srfsfvhegn fycvdvapvt vedegqwtcm aenpsgrssc
      181 tsrltvivpk aykrpefvee lralltetgt vsleckvvgv ptpvlrwfkd dkeikagdvf
      241 altanpddpt slgiytceav ncmgisysss kvhvvgkgsr egslkpadsm tpsgplpvfs
      301 rilqdeccri gdtlrlscqv qvppwpktit wynkegriea tekyhvmedg lggysievnp
      361 veamdegewk cvasstedmk qfttcyvams ipknyrkprf meslkavlte eglvsfeckv
      421 vgfptpllrw fkdgqelkpg dvyqltgtns lgsycciarn cmgeakstae ltiediqnql
      481 neeermqlls tnqppsfikg lrscearine efhftvqvsi spvpslawyr ddvpveenek
      541 yqiakenlgt chldvrklef vdqaewkcva sndfghsvts cflkliipkh ykkpkflesl
      601 railsdegav nleckvigvp qpilkwykdg velkpgdihr iisgqdgtcc lgtytceatn
      661 cmgtvsssas llgfedkfpv rkeakevqsp nghelarnls lstiheerts qlydtpqtdh
      721 svtlddrgev sfsfdgkevs vslyetpdlt eeealqivem yadqlsehvt ehnvielppm
      781 rfvkessnsg nllmeavvid vspdyfvsae dgddlrtead fedasilddv trvfssperd
      841 srgslkrsam ysledddkpp srpprkksts ssksekseks qkmesesfhs aqkdepfspv
      901 spmspvsslk qddsdtfada lssarlsise nqvqklyeag hdrkrslsad ktagssiddg
      961 iggdssfdsv tgvpkkkkhr kkkhkkekgs seessigsei dgkrrrsrsa sksdlhqrie
     1021 sinkteepyv edvpdksser kpssdsdksl sksnkerlld nmrkmkdpvl vvrdtlvnid
     1081 qldaklnses eedckvivte niikpiqnlc eevssveaka lksagdrslt qnvrisllea
     1141 iggpteellr gmeligrqen vknrktdvia ilesltdpvd eilmgitkie heltgrakge
     1201 rpialirmts avskldenir kvltvdkrat rlvssledil stldfflndv svdpskgtie
     1261 tvdtvvvesl srsvedvara lggfssnvqv skvidelltp trelkakldt lksaletyet
     1321 kgilsehrtk lieslqesvg qsiqeiqkvk ekstteeqka padqakqdil kevrltlhea
     1381 lksyeniagt ldprlstvfd rvdemyrrlr tpqkrdigie tlmnlegply slqcaltsma
     1441 ivensemvfk llepaifqlk ltitsleess lkpvmemlqs iektfsgttn eyeetissrs
     1501 vqdepqtltd rildplidvq salsaalqti edvdsvknga tspqkilass eiaaclielr
     1561 qcvsdtahta mtlredetln slidlkepll dlhlaltsed rsfqelpvir kasvplekln
     1621 vvvstvlrhc dsqesikavs evskmleeie kqlpktvtrl idaeeakrhy legmnidenl
     1681 snvhfalssv lerqeknyps rvssftncie elrqnlgssa vaianlknpf deeiveqvyr
     1741 lketllnlqr slltqehdve eeqilkdlis pieklknivq sivdsdaksd avlpvlelle
     1801 eiekdtplia kevskkmaqk eaeklipkkk kqktptfela dkisrsldpm khwlsttsee
     1861 sikedeesgl sstvselkkd vtkiaietsy sepptdesli ealidlqepl mrlrnaismy
     1921 hepadlntle glgrpmkyll qtimdvlrdh aeeealrpiv diveeienqi pisikealyk
     1981 kelkemvksm treetgatts qetiqeeivp qelpqevqvh tsqettadgt lesstvlpea
     2041 qfsesvieqt tieeissqks dqvitmieev pkqkvavdea kkkgeeeaiq lvlslsktle
     2101 nvqmdvmgil edfeqpttgt ttlpishlan sleelrktif ivgaktgdya ksvhsyeetv
     2161 eetisilksl qqplnnlqev lreshqhgar ellilnhliq plnaieeeii nrsieklnhd
     2221 skliencqpv lhllndikqs vpvvvkdiys rqelleslrg ilkplkniee rmkilenete
     2281 gtlerdvaki lvnpleylle aisvtsrelq fldqrreivi eirnlveplv eflsclsvvq
     2341 ssrkslvpea allderrnvi lkavedlqrq ttiileripn legaslfekp lislkkatas
     2401 vqkqigktdy skrpssvdft lqynlacsfd nlrstistve enadesvrqv iskslealqk
     2461 qialsqthfi qtseqpvdee avvegflypt nqlqsalnvl keqidqktia svsiesvrll
     2521 qalanstsdl asslsmhrvq lirngasegs slvetlsavv dvlqsvkdsi kaiekvviek
     2581 egagkvqeve nrsvsmdmpm dmpititqie tivdevieis keeiesvlti erlspdrdqq
     2641 rteevksdkm kaitmedesv kkeddtkdks vemmdkrkee mvepvekaei ssekmedkke
     2701 kleeveemem ltekveelak kqtdeeakis ekveqlgeqe ekakeveisv ekvdeltkke
     2761 adqvmkipev aeklevkeek meaqekevet ekvelakket dekaqqlekq netveeqikk
     2821 eekkvkvste emeelstket eskvekleek kkqieeeqkv kilsekvdel tqkktdekve
     2881 kleekvekve eqvketekge aaatkeeelv tkeadektkk leeevkmsse kqevsvkkde
     2941 kveekeedti kilsekteel avkeenvqal aeeaekltka eekievspeg tekalikeek
     3001 tevligkaee aikpeksevs steksekllq keekvetlie kkeeigetsk tekhkaeltk
     3061 evvaaekkee kikgteklee psnqeatagt sekpekltki eekvgeaekk ekiiekseea
     3121 ikkeetqalp skveelikke eitqtssska eestkeegkt veiqrttnsi itvqqplkel
     3181 tnlvssalee plsskseeek rkireltali qilydlratv ssvqnsasps sipeleehll
     3241 hllnslrtld rstekilrit ekeltpalke nvmvslqlll epldvlhref sslqetavqs
     3301 sayvslsinv rnvtetisql meairktsel krvkiveeir vlaamkkees qdieettakp
     3361 leelieaimv sqehmkpdas pfgsvspsve ptesleqqkl eeemmleanl vekivspvkn
     3421 lrelvakiek qkleeaetld lpakqttsil ntilhpleel erslnissvq qivqteetvk
     3481 etkqsvqssq svpvgavlee lkrsiatiqe evtlasvkea sktesgvlfi kevekslenl
     3541 kfslatvqkv vatdtesvee lttqqrtmav etfvksvtdl gekclaivsr pktkvdtaeg
     3601 lqkeqleevl emmvqpiqvl restseietq raeeiqhmdq ternviealk plvhpleqle
     3661 qllctavhqd ssiktetaee mketgssvek lkltpvledl qksiavveqq malqakevdt
     3721 ssvkvessva eliesplknl kstmaaiqnl eteeteemts eerttalkaf arcleeigqv
     3781 csiassqqkm ettllqqpqv aklefeqqvl eaisgpiqvl retvskidee tqeaeallvs
     3841 etkeaanvlh tlvqpleqle kclsvaveqt siageesake mkdlpasmkl nvepvlqelq
     3901 ksiatiqeqv sfetgmeksa dkletsvaka ieepleklka svaaveeivc kteqtqnlse
     3961 sekssilksf aksveeisqh cvamisqkav pktleksqva ileeaisplk alreaidkie
     4021 denlqeaekl dtleakelek slkilveplq kletsllstv eqavltqlee teemkdsvps
     4081 aekldispvv eelqkcvatv qeqvklqtat aspeekavlv qaaeksleel sssltviqsi
     4141 vatvsneeek vsdmdkmtav qqfaksvhel eekclvsqeq vevketakei egidakamet
     4201 ivapvhalre tilkiqeenl eevgtlrtee mklqvlsplv hpleelercl itavqqesgq
     4261 kletteeikk eslekvvlkp ileelkqniv ivqkhlamep essfkleavk niteaieely
     4321 ttatavqqvv aisseeaeem ktskasales faksmeqlee rclavinqqk adkiekpekq
     4381 qgklvdaqvl qkitdtihvl reslsqieee klqeakelki tkeeeeaglk lnvlieplnv
     4441 lekalmtave eskivteett qelkkteiav eklnlqpvle elqksiavvq qgtsktavsv
     4501 desdlmkmae ksleemklsm aavqelvshq aqeikaeekl eafaksmeea genllavakq
     4561 qelkkatlri eeppalkvse dfvktvvtpi aelhttivki dketklskek ekeeavifls
     4621 mvqplkklee sflsavqeei kqeaaeeirp aipvlqklpi qstlkelnkn vaeiqeqlvl
     4681 aketltrtgs tedvaviktl erslsnlrts saivqqlaai eepgqqildv envsalqafa
     4741 iaveefkksc aaiisrpavm ssiigkvhle eitkkeasst etilvpvkil qesictieel
     4801 klqetgildt seikkpttvl seliaplqql eqsfmaaiqg ehviehaaes fetdkasfek
     4861 talkpileef qksiaivqeh mileegaqsl sdaeeaslkm maqtvtdlkt svaaiqqite
     4921 tvpemanels kgesvaafet fakslhdlve raatidhqqi ilepaadtis edasslktwa
     4981 divedspvqa lepmivdqgt iespseials iseeeaqvlk glakplselr eclavvveer
     5041 ksmdanemaf amseredlsl lktvaqplle lknaavsvil eqtavesage ksfeekpetn
     5101 lnplieslee lchsialied qmlvesaedr tidevsllta laeplmtlqr svcvleervl
     5161 spdveplpee snwineclav plqeieksia eireccvvep eeqsrmvtld wsvveklvep
     5221 vevirstiig medtvaeemk kctimknliq plsevhesfm llkdrtdltv aeeeasidai
     5281 vdslsnleks isfieeqtae kvedvsssak ddrlvigtlt eslmqlkesi mtveesptvy
     5341 lenleqplke lqsalesvsn irrekaekek tkkivsleea trvstrlkns ikeindsies
     5401 idnkiesfkg llgievqien ealkslakpl eglkggieki lekeevegst vdslrklrna
     5461 igiirqqsad kplaesfhfe tvdivgmlrp ltpclhelet ctekvetawk ensageglve
     5521 letpisnlmd tinilrekll rldivwvtee ekkkkearkk eeekaeklkq eeeehkkkee
     5581 aeklkqeeee rkkkaeaerl qreeeerkkk eeaerlkqee eerkkkeete rlkqeeeerq
     5641 rkeeterlkq eeekrekkee peklkqeaee hrkkeeaerl kqeeeckkke eaeklkqaee
     5701 egikreeeer kqeeeklrqe gearkkkeee ekikqeeetr kikekeeklk qeeeerkkke
     5761 deeklrqeee arkrkeerlk leeerkkkee geklqleqer kkkeeedklk qaeeerkkke
     5821 eeerqwkeke eklkqeeetl kqeeearkkk eeqerlkqqe ekrkkkeeed nlkreeeerk
     5881 nkekaeklkq keeklkreee erkkkeeqdr lkqqeeerkk keeeeklkle eqerkkreea
     5941 eklkqeeelk kkqeaeklkq eeerkkkeea eklkaeeerk kkeeaeklkq eeerkkkeea
     6001 eklkaeeerk kkeeaeklkq eeerkkkeea eklkaeeerk kkeeaeklkq eeerkkkqea
     6061 eklkaeeerk kkeeaeklkq keerkkkeea eklkaeeere kkeeaeklkq eeerkkkeea
     6121 eklkaekelk keeeaeklkq eeerkkkeea eklkaeeerk kkeeaeklkq eeerkkkeea
     6181 eklkaekelk keeeaeklkq eeerkkkeea eklkaeeerk kkeeaeklkq eeerkkkeea
     6241 eklkaeeelk kkdeavkqke dkkhkkkeae kleteekhkr keeaekqkee edrkkkeaeq
     6301 lkeqeerkkr eeaeklkqeq enrkleeesk kkkeeqerkq keqaeklkqe eaeiskkaes
     6361 dtlkkkeekl krkkeelkqq reederkeke eaekrrkeqe qrkkereera kkeeeerlkr
     6421 eeeerkrkee kakqkkeeen rrkaeeaerl kkeqekeeqr reetkrrree qekqirqete
     6481 kirkaeeerl kkedetrerr rkerdlrrqe eeeklhkeee ertkreeerr rrrreaerqw
     6541 redeeairrk eterlerrra edrqnreeme rlkredeerr rrrdedrqtr heetvkalke
     6601 ehdrlrkrqe eetsrlrkrr qeqirddsws rmrqsesdql lkkmtddsfr lgtsktfssd
     6661 ldfhrdksyk ydsgfesals sasrsyswrd sltsltrrrv ddfidyklrd tsmdryyfdt
     6721 ggyyrrrrkr ddritrarsi sllkyddyst gdsdttitpa spilsrysrt ktrsrndldd
     6781 rgsnlslylp pikdeitpre kgkkpsfctr ltnrtvgvgm rtrltctvlg hpeprvywtk
     6841 dgekletisn kyrirfdngm ayfelhealp edsglytcva envhgiaste salkvypdfq
     6901 palspptftr sikdtyrpsd nelilecrvr ghpipliswl kdgcilqgdr ykqcyldddi
     6961 yrleiaapns kdngrytcra mndlrteeis hvvqfnerdr rvtskhekly ddhfsieafr
     7021 rprfsnyltd ysvptggtva lqvevkgvpa pevrwfcgek kepvsipkak tftesgvhtl
     7081 vlpevteser gtymcraina yghvdsiaiv dvispsgsds gkpamfvsrp vdksitvtsg
     7141 edvsvsfrfs gvpkpkiiwm kglkditdgp rshkeviddy vrltlkrvvp sdegtycill
     7201 knrygcdrsf fslkvkqrar sltpdwstls srtetgslhg issesrddem syvknvpgpi
     7261 ssepvvvdgg knwlslswgk aerrgpapvi ayrvdawllg sdggarwvel gmtpinafda
     7321 fnlkpggeyk fqvtprnryg wgesvtmtns vrvsetvdlp eftkilpgql kilegttvkl
     7381 eceirvdshv dvkwyreste idprysifyn gsrcsltiek vqesdngryv ceasnlvgka
     7441 ssfarvlvvd dpkiieadak lksrllgddt edrppeftmr irdrrvqaty pvrltcqvtg
     7501 qpvpvvtwyk ndteirqder hifwdddsnf htleithstl edsgcymvta knangsvscr
     7561 cilvvdkgir ayiapeflcg ldaaytvklg gdlrmtaqie aypsvgvvwh rdgnrlrpsr
     7621 rtvmtlnhdg tvelslakvt ardagvycct atnevghaet strvsiigte tegeqtsldg
     7681 vptvtlassd ipyskeplfv tkplsteaie gdaviiscev vgdpkpevmw lrdflkpdyy
     7741 rdaaqfrlvg egpqyrleip yakldftgty sviarnchge akavislqiy akgqgkeekm
     7801 kksgvthgnv ltlpvitrel rdlrccdgda vtleckvqat peaplvrwer ggkilqisgd
     7861 fssefdgeta rlsiqhvype degeytcvay ndlgkaftsa clivdvpegk envlcqrltr
     7921 pagllsagst prstprstpi rslspavshg relrspqllp rsrsmlrrpk isppkfyavp
     7981 hnrvveeget vrfqcavvgh ptpwvkwdkn glavtpttri sikerddvki leivevtqed
     8041 aglyrviaen dygrieasar leiinrqllg pvsrtirtrs asprtypsfg rslldttsri
     8101 ngrlyldcsi rgtpsptptw frngrplers nrikryfdgk takveiskvk asdageytcv
     8161 atnvlgstkn tcqvivldph dpstsdknap kflqslpdes ivmeghcyel qtrltgtppf
     8221 svtwlkndre ipdndcckyi iygdggialr lsnvcsedag eytclvrnnf geascnglfa
     8281 vqdykgvpkl apqftktpls vitskgetac fcarvqcgkp meiawtingk dvkenprcki
     8341 eqddnvsilr iqdvsardvg dirctasvig kgpsisctaq lqlnrpvrni edsaskcnna
     8401 qaeirngrpp pkpgnftrnn slrktardvp slsqnqrnes pmrsrssslp lrtspqrssp
     8461 aptrrntpsp lpdtrkasen klmkkernfk kleriqkdek vplsskdeea tnkppddkpr
     8521 lknseddeek csmslnsdfs ktengrkrsq dngnkhddtp iicmecavee pdeaskkdqe
     8581 tvtkaekvds vrkeflaasi ikvpadvtvf tgnrvvlrvt yrghpeprvr wfragreltp
     8641 nkktaitygg gvsclisddv tadnagkyev svenefgkdr rcfsvavegp pdppagipsv
     8701 ccspgtvtin wrsspydggc tvtgytvems hpgentwtri aesclslslt apasgtnapi
     8761 pgekyrfrvr aenihgvsep gdesefvrip kegemclqdd eeefeaafea rlvemedgql
     8821 fnnryevlee lgkgrygvvk rvversssts faakfvrtik skdkeqvree ikimnmlrhp
     8881 kllllaaaye spreivmvte yisggelfer vvaddftlte rdsilfmrqi cegveymhqn
     8941 nvvhldlkpe nimcrtrtsh qiklidfgla qtlkpdtpir vlfgtpefip peiisyepig
     9001 tesdmwsvgv icyvlltgls pfmgdndaet fanitradyd ledqafdais ndakdfisgl
     9061 lvkrkelrms arqclehpwm aqhaeamsri alpteklkkf ivrrkwqktg nairalgrma
     9121 ilsansrrsp tgtaesspts erpnpidspr pinfdvsptt eskkhdiddr tekkkstges
     9181 dvqernvysf cyktevnilp eetldssere earvssdlde aslknepawd rvrscsrgng
     9241 seeiqetdts eeaqkpdeah leepvpmeki eeeteeiitr cryeeritik eetlfvqnfe
     9301 perntlnies ystsmtscne edysnnisee vtssinketd vpeankvkti rkieekride
     9361 eisetekvsk ekidqklrrv frgdsrdsgi gdcisnlsss sqqvnelgis sikeeetdnd
     9421 nnngnarsvs kkveqfenrk ifvekmtdne mtqensdvsd pkkctkgsie vdtlsdvtki
     9481 nrqheavnei edprlligrv rskfvptgnv srtaklfeke ataskspanv pqvpqrtypa
     9541 vpvvagkpqn eriqkafafw nk
//
LOCUS       XP_034183043            9142 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605608 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183043
VERSION     XP_034183043.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327152.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183043.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..9142
                     /product="uncharacterized protein LOC117605608 isoform X3"
                     /calculated_mol_wt=1039394
     Region          24..111
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          122..211
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          139..143
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          152..156
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          177..181
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          191..196
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          204..207
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          220..299
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          236..240
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          249..253
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          265..269
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          279..284
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          292..295
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          322..399
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          339..343
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          353..357
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          379..383
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          393..398
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          423..498
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          439..443
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          452..456
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          468..472
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          478..483
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          491..494
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          520..609
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          537..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          550..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          576..580
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          590..595
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          603..606
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          619..696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          635..639
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          648..652
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          664..668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          678..683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          691..694
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <2174..>2305
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <2593..3231
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2931..3801
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          3812..4597
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          4412..5192
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          6830..6920
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6847..6851
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6860..6864
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6886..6890
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6900..6905
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6913..6916
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6930..7007
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          7047..7133
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7064..7068
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7077..7081
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7103..7107
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7117..7122
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7130..7133
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7160..7240
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7168..7172
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7181..7185
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7206..7210
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7220..7225
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7233..7236
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7282..7371
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(7282,7350,7365)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(7366..7367,7369..7370)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          7392..7473
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          7403..7407
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7416..7420
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7439..7443
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7453..7458
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7466..7469
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7500..7590
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7517..7521
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7530..7534
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7556..7560
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7570..7575
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7583..7586
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7600..7691
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7618..7622
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7631..7635
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7657..7661
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7671..7676
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7684..7687
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7722..7814
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7739..7743
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7752..7756
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7780..7784
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7794..7799
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7807..7812
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7839..7929
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7856..7860
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7870..7874
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7895..7899
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7909..7914
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7999..8088
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8016..8020
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8029..8033
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8054..8058
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8068..8073
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8081..8084
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8112..8201
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          8142..8146
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8167..8171
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8181..8186
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8215..8301
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8233..8237
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8246..8250
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8272..8276
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8286..8291
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8317..8407
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8334..8338
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8347..8351
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8372..8376
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8386..8391
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8401..8404
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          8624..8704
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          8640..8644
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          8653..8657
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          8678..8682
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          8692..8697
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          8716..8812
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(8716,8786,8801)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(8802..8803,8805..8806)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          8856..9105
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(8856..8860,8862,8864,8877,8879,8908,8924..8925,8927,
                     8931,8933,8971,8973,8975..8976,8978,8990..8991,8994,
                     9009..9012)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(8856..8860,8862,8864,8877,8879,8908,8924..8925,8927,
                     8931,8975..8976,8978,8990..8991)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(8860,8931,8933,8971,8973,8975,8994,9009..9012)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(8990..8998,9001..9012)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..9142
                     /gene="LOC117605608"
                     /coded_by="XM_034327152.2:209..27637"
                     /db_xref="GeneID:117605608"
ORIGIN      
        1 mtltdqtaka kgpprwldpd qvlsvqgrtg edvvfepklr wsdvfdelrf eliredvpip
       61 sdryrvqmrg nvvqltlkqs qkddtghyal vatrlgqgfd kgsskkihls vdepiseegd
      121 ppvflrrltd lavkvgtrtr flveirsstt pkitwhrnae pvhagsrfsf vhegnfycvd
      181 vapvtvedeg qwtcmaenps grssctsrlt vivpkaykrp efveelrall tetgtvslec
      241 kvvgvptpvl rwfkddkeik agdvfaltan pddptslgiy tceavncmgi syssskvhvv
      301 gkgsregslk padsmtpsgp lpvfsrilqd eccrigdtlr lscqvqvppw pktitwynke
      361 grieatekyh vmedglggys ievnpveamd egewkcvass tedmkqfttc yvamsipkny
      421 rkprfmeslk avlteeglvs feckvvgfpt pllrwfkdgq elkpgdvyql tgtnslgsyc
      481 ciarncmgea kstaeltied iqnqlneeer mqllstnqpp sfikglrsce arineefhft
      541 vqvsispvps lawyrddvpv eenekyqiak enlgtchldv rklefvdqae wkcvasndfg
      601 hsvtscflkl iipkhykkpk fleslrails degavnleck vigvpqpilk wykdgvelkp
      661 gdihriisgq dgtcclgtyt ceatncmgtv sssasllgfe dkfpvrkeak evqspnghel
      721 arnlslstih eertsqlydt pqtdhsvtld drgevsfsfd gkevsvslye tpdlteeeal
      781 qivemyadql sehvtehnvi elppmrfvke ssnsgnllme avvidvspdy fvsaedgddl
      841 rteadfedas ilddvtrvfs sperdsrgsl krsamysled ddkppsrppr kkstssskse
      901 kseksqkmes esfhsaqkde pfspvspmsp vsslkqddsd tfadalssar lsisenqvqk
      961 lyeaghdrkr slsadktags siddgiggds sfdsvtgvpk kkkhrkkkhk kekgsseess
     1021 igseidgkrr rsrsasksdl hqriesinkt eepyvedvpd ksserkpssd sdkslsksnk
     1081 erlldnmrkm kdpvlvvrdt lvnidqldak lnseseedck vivteniikp iqnlceevss
     1141 veakalksag drsltqnvri slleaiggpt eellrgmeli grqenvknrk tdviailesl
     1201 tdpvdeilmg itkieheltg rakgerpial irmtsavskl denirkvltv dkratrlvss
     1261 ledilstldf flndvsvdps kgtietvdtv vveslsrsve dvaralggfs snvqvskvid
     1321 elltptrelk akldtlksal etyetkgils ehrtkliesl qesvgqsiqe iqkvkekstt
     1381 eeqkapadqa kqdilkevrl tlhealksye niagtldprl stvfdrvdem yrrlrtpqkr
     1441 digietlmnl egplyslqca ltsmaivens emvfkllepa ifqlkltits leesslkpvm
     1501 emlqsiektf sgttneyeet issrsvqdep qtltdrildp lidvqsalsa alqtiedvds
     1561 vkngatspqk ilasseiaac lielrqcvsd tahtamtlre detlnslidl keplldlhla
     1621 ltsedrsfqe lpvirkasvp leklnvvvst vlrhcdsqes ikavsevskm leeiekqlpk
     1681 tvtrlidaee akrhylegmn idenlsnvhf alssvlerqe knypsrvssf tncieelrqn
     1741 lgssavaian lknpfdeeiv eqvyrlketl lnlqrslltq ehdveeeqil kdlispiekl
     1801 knivqsivds daksdavlpv lelleeiekd tpliakevsk kmaqkeaekl ipkkkkqktp
     1861 tfeladkisr sldpmkhwls ttseesiked eesglsstvs elkkdvtkia ietsyseppt
     1921 desliealid lqeplmrlrn aismyhepad lntleglgrp mkyllqtimd vlrdhaeeea
     1981 lrpivdivee ienqipisik ealykkelke mvksmtreet gattsqetiq eeivpqelpq
     2041 evqvhtsqet tadgtlesst vlpeaqfses vieqttieei ssqksdqvit mieevpkqkv
     2101 avdeakkkge eeaiqlvlsl sktlenvqmd vmgiledfeq pttgtttlpi shlansleel
     2161 rktifivgak tgdyaksvhs yeetveetis ilkslqqpln nlqevlresh qhgarellil
     2221 nhliqplnai eeeiinrsie klnhdsklie ncqpvlhlln dikqsvpvvv kdiysrqell
     2281 eslrgilkpl knieermkil enetegtler dvakilvnpl eylleaisvt srelqfldqr
     2341 reivieirnl veplveflsc lsvvqssrks lvpeaallde rrnvilkave dlqrqttiil
     2401 eripnlegas lfekplislk katasvqkqi gktdyskrps svdftlqynl acsfdnlrst
     2461 istveenade svrqvisksl ealqkqials qthfiqtseq pvdeeavveg flyptnqlqs
     2521 alnvlkeqid qktiasvsie svrllqalan stsdlassls mhrvqlirng asegsslvet
     2581 lsavvdvlqs vkdsikaiek vviekegagk vqevenrsvs mdmpmdmpit itqietivde
     2641 vieiskeeie svltierlsp drdqqrteev ksdkmkaitm edesvkkedd tkdksvemmd
     2701 krkeemvepv ekaeissekm edkkekleev eememltekv eelakkqtde eakisekveq
     2761 lgeqeekake veisvekvde ltkkeadqvm kipevaekle vkeekmeaqe kevetekvel
     2821 akketdekaq qlekqnetve eqikkeekkv kvsteemeel stketeskve kleekkkqie
     2881 eeqkvkilse kvdeltqkkt dekvekleek vekveeqvke tekgeaaatk eeelvtkead
     2941 ektkkleeev kmssekqevs vkkdekveek eedtikilse kteelavkee nvqalaeeae
     3001 kltkaeekie vspegtekal ikeektevli gkaeeaikpe ksevssteks ekllqkeekv
     3061 etliekkeei getsktekhk aeltkevvaa ekkeekikgt ekleepsnqe atagtsekpe
     3121 kltkieekvg eaekkekiie kseeaikkee tqalpskvee likkeeitqt ssskaeestk
     3181 eegktveiqr ttnsiitvqq plkeltnlvs saleeplssk seeekrkire ltaliqilyd
     3241 lratvssvqn saspssipel eehllhllns lrtldrstek ilritekelt palkenvmvs
     3301 lqlllepldv lhrefsslqe tavqssayvs lsinvrnvte tisqlmeair ktselkrvki
     3361 veeirvlaam kkeesqdiee ttakpleeli eaimvsqehm kpdaspfgsv spsveptesl
     3421 eqqkleeemm leanlvekiv spvknlrelv akiekqklee aetldlpakq ttsilntilh
     3481 pleelersln issvqqivqt eetvketkqs vqssqsvpvg avleelkrsi atiqeevtla
     3541 svkeasktes gvlfikevek slenlkfsla tvqkvvatdt esveelttqq rtmavetfvk
     3601 svtdlgekcl aivsrpktkv dtaeglqkeq leevlemmvq piqvlrests eietqraeei
     3661 qhmdqternv iealkplvhp leqleqllct avhqdssikt etaeemketg ssveklkltp
     3721 vledlqksia vveqqmalqa kevdtssvkv essvaelies plknlkstma aiqnleteet
     3781 eemtseertt alkafarcle eigqvcsias sqqkmettll qqpqvaklef eqqvleaisg
     3841 piqvlretvs kideetqeae allvsetkea anvlhtlvqp leqlekclsv aveqtsiage
     3901 esakemkdlp asmklnvepv lqelqksiat iqeqvsfetg meksadklet svakaieepl
     3961 eklkasvaav eeivckteqt qnlsesekss ilksfaksve eisqhcvami sqkavpktle
     4021 ksqvaileea isplkalrea idkiedenlq eaekldtlea kelekslkil veplqklets
     4081 llstveqavl tqleeteemk dsvpsaekld ispvveelqk cvatvqeqvk lqtataspee
     4141 kavlvqaaek sleelssslt viqsivatvs neeekvsdmd kmtavqqfak svheleekcl
     4201 vsqeqvevke takeiegida kametivapv halretilki qeenleevgt lrteemklqv
     4261 lsplvhplee lerclitavq qesgqklett eeikkeslek vvlkpileel kqnivivqkh
     4321 lamepessfk leavknitea ieelyttata vqqvvaisse eaeemktska salesfaksm
     4381 eqleerclav inqqkadkie kpekqqgklv daqvlqkitd tihvlresls qieeeklqea
     4441 kelkitkeee eaglklnvli eplnvlekal mtaveeskiv teettqelkk teiaveklnl
     4501 qpvleelqks iavvqqgtsk tavsvdesdl mkmaekslee mklsmaavqe lvshqaqeik
     4561 aeekleafak smeeagenll avakqqelkk atlrieeppa lkvsedfvkt vvtpiaelht
     4621 tivkidketk lskekekeea viflsmvqpl kkleesflsa vqeeikqeaa eeirpaipvl
     4681 qklpiqstlk elnknvaeiq eqlvlaketl trtgstedva viktlersls nlrtssaivq
     4741 qlaaieepgq qildvenvsa lqafaiavee fkkscaaiis rpavmssiig kvhleeitkk
     4801 easstetilv pvkilqesic tieelklqet gildtseikk pttvlselia plqqleqsfm
     4861 aaiqgehvie haaesfetdk asfektalkp ileefqksia ivqehmilee gaqslsdaee
     4921 aslkmmaqtv tdlktsvaai qqitetvpem anelskgesv aafetfaksl hdlveraati
     4981 dhqqiilepa adtisedass lktwadived spvqalepmi vdqgtiesps eialsiseee
     5041 aqvlkglakp lselreclav vveerksmda nemafamser edlsllktva qpllelknaa
     5101 vsvileqtav esageksfee kpetnlnpli esleelchsi aliedqmlve saedrtidev
     5161 slltalaepl mtlqrsvcvl eervlspdve plpeesnwin eclavplqei eksiaeirec
     5221 cvvepeeqsr mvtldwsvve klvepvevir stiigmedtv aeemkkctim knliqplsev
     5281 hesfmllkdr tdltvaeeea sidaivdsls nleksisfie eqtaekvedv sssakddrlv
     5341 igtlteslmq lkesimtvee sptvylenle qplkelqsal esvsnirrek aekektkkiv
     5401 sleeatrvst rlknsikein dsiesidnki esfkgllgie vqienealks lakpleglkg
     5461 giekilekee vegstvdslr klrnaigiir qqsadkplae sfhfetvdiv gmlrpltpcl
     5521 heletctekv etawkensag eglveletpi snlmdtinil rekllrldiv wvteeekkkk
     5581 earkkeeeka eklkqeeeeh kkkeeaeklk qeeeerkkka eaerlqreee erkkkeeaer
     5641 lkqeeeerkk keeterlkqe eeerqrkeet erlkqeeekr ekkeepeklk qeaeehrkke
     5701 eaerlkqeee ckkkeeaekl kqaeeegikr eeeerkqeee klrqegeark kkeeeekikq
     5761 eeetrkikek eeklkqeeee rkkkedeekl rqeeearkrk eerlkleeer kkkeegeklq
     5821 leqerkkkee edklkqaeee rkkkeeeerq wkekeeklkq eeetlkqeee arkkkeeqer
     5881 lkqqeekrkk keeednlkre eeerknkeka eklkqkeekl kreeeerkkk eeqdrlkqqe
     5941 eerkkkeeee klkleeqerk kreeaeklkq eeelkkkqea eklkqeeerk kkeeaeklka
     6001 eeerkkkeea eklkqeeerk kkeeaeklka eeerkkkeea eklkqeeerk kkeeaeklka
     6061 eeerkkkeea eklkqeeerk kkqeaeklka eeerkkkeea eklkqkeerk kkeeaeklka
     6121 eeerekkeea eklkqeeerk kkeeaeklka ekelkkeeea eklkqeeerk kkeeaeklka
     6181 eeerkkkeea eklkqeeerk kkeeaeklka ekelkkeeea eklkqeeerk kkeeaeklka
     6241 eeerkkkeea eklkqeeerk kkeeaeklka eeelkkkdea vkqkedkkhk kkeaeklete
     6301 ekhkrkeeae kqkeeedrkk keaeqlkeqe erkkreeaek lkqeqenrkl eeeskkkkee
     6361 qerkqkeqae klkqeeaeis kkaesdtlkk keeklkrkke elkqqreede rkekeeaekr
     6421 rkeqeqrkke reerakkeee erlkreeeer krkeekakqk keeenrrkae eaerlkkeqe
     6481 keeqrreetk rrreeqekqi rqetekirka eeerlkkede trerrrkerd lrrqeeeekl
     6541 hkeeeertkr eeerrrrrre aerqwredee airrketerl errraedrqn reemerlkre
     6601 deerrrrrde drqtrheetv kalkeehdrl rkrqeeetsr lrkrrqeqir ddswsrmrqs
     6661 esdqllkkmt ddsfrlgtsk tfssdldfhr dksykydsgf esalssasrs yswrdsltsl
     6721 trrrvddfid yklrdtsmdr yyfdtggyyr rrrkrddrit rarsisllky ddystgdsdt
     6781 titpaspils rysrtktrsr ndlddrgsnl slylppikde itprekgkkp sfctrltnrt
     6841 vgvgmrtrlt ctvlghpepr vywtkdgekl etisnkyrir fdngmayfel healpedsgl
     6901 ytcvaenvhg iastesalkv ypdfqpalsp ptftrsikdt yrpsdnelil ecrvrghpip
     6961 liswlkdgci lqgdrykqcy ldddiyrlei aapnskdngr ytcramndlr teeishvvqf
     7021 nerdrrvtsk heklyddhfs ieafrrprfs nyltdysvpt ggtvalqvev kgvpapevrw
     7081 fcgekkepvs ipkaktftes gvhtlvlpev tesergtymc rainayghvd siaivdvisp
     7141 sgsdsgkpam fvsrpvdksi tvtsgedvsv sfrfsgvpkp kiiwmkglkd itdgprshke
     7201 viddyvrltl krvvpsdegt ycillknryg cdrsffslkv kqrarsltpd wstlssrtet
     7261 gslhgisses rddemsyvkn vpgpissepv vvdggknwls lswgkaerrg papviayrvd
     7321 awllgsdgga rwvelgmtpi nafdafnlkp ggeykfqvtp rnrygwgesv tmtnsvrvse
     7381 tvdlpeftki lpgqlkileg ttvkleceir vdshvdvkwy resteidpry sifyngsrcs
     7441 ltiekvqesd ngryvceasn lvgkassfar vlvvddpkii eadaklksrl lgddtedrpp
     7501 eftmrirdrr vqatypvrlt cqvtgqpvpv vtwykndtei rqderhifwd ddsnfhtlei
     7561 thstledsgc ymvtaknang svscrcilvv dkgirayiap eflcgldaay tvklggdlrm
     7621 taqieaypsv gvvwhrdgnr lrpsrrtvmt lnhdgtvels lakvtardag vycctatnev
     7681 ghaetstrvs iigtetegeq tsldgvptvt lassdipysk eplfvtkpls teaiegdavi
     7741 iscevvgdpk pevmwlrdfl kpdyyrdaaq frlvgegpqy rleipyakld ftgtysviar
     7801 nchgeakavi slqiyakgqg keekmkksgv thgnvltlpv itrelrdlrc cdgdavtlec
     7861 kvqatpeapl vrwerggkil qisgdfssef dgetarlsiq hvypedegey tcvayndlgk
     7921 aftsaclivd vpegkenvlc qrltrpagll sagstprstp rstpirslsp avshgrelrs
     7981 pqllprsrsm lrrpkisppk fyavphnrvv eegetvrfqc avvghptpwv kwdknglavt
     8041 pttrisiker ddvkileive vtqedaglyr viaendygri easarleiin rqllgpvsrt
     8101 irtrsasprt ypsfgrslld ttsringrly ldcsirgtps ptptwfrngr plersnrikr
     8161 yfdgktakve iskvkasdag eytcvatnvl gstkntcqvi vldphdpsts dknapkflqs
     8221 lpdesivmeg hcyelqtrlt gtppfsvtwl kndreipdnd cckyiiygdg gialrlsnvc
     8281 sedageytcl vrnnfgeasc nglfavqdyk gvpklapqft ktplsvitsk getacfcarv
     8341 qcgkpmeiaw tingkdvken prckieqddn vsilriqdvs ardvgdirct asvigkgpsi
     8401 sctaqlqlnr pvrniedsas kcnnaqaeir ngrpppkpgn ftrnnslrkt ardvpslsqn
     8461 qrnespmrsr ssslplrtsp qrsspaptrr ntpsplpdtr kasenklmkk ernfkkleri
     8521 qkdekvplss kdeeatnkpp ddkprlknse ddeekcsmsl nsdfskteng rkrsqdngnk
     8581 hddtpiicme caveepdeas kkdqetvtka ekvdsvrkef laasiikvpa dvtvftgnrv
     8641 vlrvtyrghp eprvrwfrag reltpnkkta itygggvscl isddvtadna gkyevsvene
     8701 fgkdrrcfsv avegppdppa gipsvccspg tvtinwrssp ydggctvtgy tvemshpgen
     8761 twtriaescl slsltapasg tnapipgeky rfrvraenih gvsepgdese fvripkegem
     8821 clqddeeefe aafearlvem edgqlfnnry evleelgkgr ygvvkrvver ssstsfaakf
     8881 vrtikskdke qvreeikimn mlrhpkllll aaayesprei vmvteyisgg elfervvadd
     8941 ftlterdsil fmrqicegve ymhqnnvvhl dlkpenimcr trtshqikli dfglaqtlkp
     9001 dtpirvlfgt pefippeiis yepigtesdm wsvgvicyvl ltglspfmgd ndaetfanit
     9061 radydledqa fdaisndakd fisgllvkrk elrmsarqcl ehpwmaqhae amsrialpte
     9121 klkkfivrrk wqrstklrtq gy
//
LOCUS       XP_076546015            8783 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605608 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546015
VERSION     XP_076546015.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689900.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..8783
                     /product="uncharacterized protein LOC117605608 isoform X4"
                     /calculated_mol_wt=999592
     Region          <1789..2427
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2127..2997
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          3008..3793
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          3608..4388
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          6026..6116
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6043..6047
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6056..6060
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6082..6086
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6096..6101
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6109..6112
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6126..6203
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          6243..6329
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6260..6264
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6273..6277
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6299..6303
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6313..6318
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6326..6329
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6356..6436
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6364..6368
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6377..6381
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6402..6406
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6416..6421
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6429..6432
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6478..6567
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(6478,6546,6561)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(6562..6563,6565..6566)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          6588..6669
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          6599..6603
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6612..6616
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6635..6639
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6649..6654
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6662..6665
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6696..6786
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          6713..6717
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6726..6730
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6752..6756
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6766..6771
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6779..6782
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6796..6887
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6814..6818
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6827..6831
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6853..6857
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6867..6872
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          6880..6883
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          6918..7010
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          6935..6939
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          6948..6952
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          6976..6980
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          6990..6995
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7003..7008
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7035..7125
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7052..7056
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7066..7070
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7091..7095
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7105..7110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7195..7284
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7212..7216
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7225..7229
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7250..7254
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7264..7269
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7277..7280
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7308..7397
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          7338..7342
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7363..7367
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7377..7382
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7411..7497
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7429..7433
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7442..7446
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7468..7472
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7482..7487
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7513..7603
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7530..7534
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7543..7547
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7568..7572
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7582..7587
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7597..7600
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          7820..7900
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          7836..7840
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          7849..7853
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          7874..7878
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          7888..7893
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          7912..8008
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(7912,7982,7997)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(7998..7999,8001..8002)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          8052..8301
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(8052..8056,8058,8060,8073,8075,8104,8120..8121,8123,
                     8127,8129,8167,8169,8171..8172,8174,8186..8187,8190,
                     8205..8208)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(8052..8056,8058,8060,8073,8075,8104,8120..8121,8123,
                     8127,8171..8172,8174,8186..8187)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(8056,8127,8129,8167,8169,8171,8190,8205..8208)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(8186..8194,8197..8208)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..8783
                     /gene="LOC117605608"
                     /coded_by="XM_076689900.1:190..26541"
                     /db_xref="GeneID:117605608"
ORIGIN      
        1 mrfvkessns gnllmeavvi dvspdyfvsa edgddlrtea dfedasildd vtrvfssper
       61 dsrgslkrsa mysledddkp psrpprkkst ssskseksek sqkmesesfh saqkdepfsp
      121 vspmspvssl kqddsdtfad alssarlsis enqvqklyea ghdrkrslsa dktagssidd
      181 giggdssfds vtgvpkkkkh rkkkhkkekg sseessigse idgkrrrsrs asksdlhqri
      241 esinkteepy vedvpdksse rkpssdsdks lsksnkerll dnmrkmkdpv lvvrdtlvni
      301 dqldaklnse seedckvivt eniikpiqnl ceevssveak alksagdrsl tqnvrislle
      361 aiggpteell rgmeligrqe nvknrktdvi ailesltdpv deilmgitki eheltgrakg
      421 erpialirmt savskldeni rkvltvdkra trlvssledi lstldfflnd vsvdpskgti
      481 etvdtvvves lsrsvedvar alggfssnvq vskvidellt ptrelkakld tlksaletye
      541 tkgilsehrt klieslqesv gqsiqeiqkv kekstteeqk apadqakqdi lkevrltlhe
      601 alksyeniag tldprlstvf drvdemyrrl rtpqkrdigi etlmnlegpl yslqcaltsm
      661 aivensemvf kllepaifql kltitslees slkpvmemlq siektfsgtt neyeetissr
      721 svqdepqtlt drildplidv qsalsaalqt iedvdsvkng atspqkilas seiaacliel
      781 rqcvsdtaht amtlredetl nslidlkepl ldlhlaltse drsfqelpvi rkasvplekl
      841 nvvvstvlrh cdsqesikav sevskmleei ekqlpktvtr lidaeeakrh ylegmniden
      901 lsnvhfalss vlerqeknyp srvssftnci eelrqnlgss avaianlknp fdeeiveqvy
      961 rlketllnlq rslltqehdv eeeqilkdli spieklkniv qsivdsdaks davlpvlell
     1021 eeiekdtpli akevskkmaq keaeklipkk kkqktptfel adkisrsldp mkhwlsttse
     1081 esikedeesg lsstvselkk dvtkiaiets ysepptdesl iealidlqep lmrlrnaism
     1141 yhepadlntl eglgrpmkyl lqtimdvlrd haeeealrpi vdiveeienq ipisikealy
     1201 kkelkemvks mtreetgatt sqetiqeeiv pqelpqevqv htsqettadg tlesstvlpe
     1261 aqfsesvieq ttieeissqk sdqvitmiee vpkqkvavde akkkgeeeai qlvlslsktl
     1321 envqmdvmgi ledfeqpttg tttlpishla nsleelrkti fivgaktgdy aksvhsyeet
     1381 veetisilks lqqplnnlqe vlreshqhga rellilnhli qplnaieeei inrsieklnh
     1441 dskliencqp vlhllndikq svpvvvkdiy srqelleslr gilkplknie ermkilenet
     1501 egtlerdvak ilvnpleyll eaisvtsrel qfldqrreiv ieirnlvepl veflsclsvv
     1561 qssrkslvpe aallderrnv ilkavedlqr qttiilerip nlegaslfek plislkkata
     1621 svqkqigktd yskrpssvdf tlqynlacsf dnlrstistv eenadesvrq viskslealq
     1681 kqialsqthf iqtseqpvde eavvegflyp tnqlqsalnv lkeqidqkti asvsiesvrl
     1741 lqalanstsd lasslsmhrv qlirngaseg sslvetlsav vdvlqsvkds ikaiekvvie
     1801 kegagkvqev enrsvsmdmp mdmpititqi etivdeviei skeeiesvlt ierlspdrdq
     1861 qrteevksdk mkaitmedes vkkeddtkdk svemmdkrke emvepvekae issekmedkk
     1921 ekleeveeme mltekveela kkqtdeeaki sekveqlgeq eekakeveis vekvdeltkk
     1981 eadqvmkipe vaeklevkee kmeaqekeve tekvelakke tdekaqqlek qnetveeqik
     2041 keekkvkvst eemeelstke teskveklee kkkqieeeqk vkilsekvde ltqkktdekv
     2101 ekleekvekv eeqvketekg eaaatkeeel vtkeadektk kleeevkmss ekqevsvkkd
     2161 ekveekeedt ikilsektee lavkeenvqa laeeaekltk aeekievspe gtekalikee
     2221 ktevligkae eaikpeksev ssteksekll qkeekvetli ekkeeigets ktekhkaelt
     2281 kevvaaekke ekikgtekle epsnqeatag tsekpekltk ieekvgeaek kekiieksee
     2341 aikkeetqal pskveelikk eeitqtsssk aeestkeegk tveiqrttns iitvqqplke
     2401 ltnlvssale eplsskseee krkireltal iqilydlrat vssvqnsasp ssipeleehl
     2461 lhllnslrtl drstekilri tekeltpalk envmvslqll lepldvlhre fsslqetavq
     2521 ssayvslsin vrnvtetisq lmeairktse lkrvkiveei rvlaamkkee sqdieettak
     2581 pleelieaim vsqehmkpda spfgsvspsv eptesleqqk leeemmlean lvekivspvk
     2641 nlrelvakie kqkleeaetl dlpakqttsi lntilhplee lerslnissv qqivqteetv
     2701 ketkqsvqss qsvpvgavle elkrsiatiq eevtlasvke asktesgvlf ikevekslen
     2761 lkfslatvqk vvatdtesve elttqqrtma vetfvksvtd lgekclaivs rpktkvdtae
     2821 glqkeqleev lemmvqpiqv lrestseiet qraeeiqhmd qternvieal kplvhpleql
     2881 eqllctavhq dssiktetae emketgssve klkltpvled lqksiavveq qmalqakevd
     2941 tssvkvessv aeliesplkn lkstmaaiqn leteeteemt seerttalka farcleeigq
     3001 vcsiassqqk mettllqqpq vaklefeqqv leaisgpiqv lretvskide etqeaeallv
     3061 setkeaanvl htlvqpleql ekclsvaveq tsiageesak emkdlpasmk lnvepvlqel
     3121 qksiatiqeq vsfetgmeks adkletsvak aieepleklk asvaaveeiv ckteqtqnls
     3181 esekssilks faksveeisq hcvamisqka vpktleksqv aileeaispl kalreaidki
     3241 edenlqeaek ldtleakele kslkilvepl qkletsllst veqavltqle eteemkdsvp
     3301 saekldispv veelqkcvat vqeqvklqta taspeekavl vqaaekslee lsssltviqs
     3361 ivatvsneee kvsdmdkmta vqqfaksvhe leekclvsqe qvevketake iegidakame
     3421 tivapvhalr etilkiqeen leevgtlrte emklqvlspl vhpleelerc litavqqesg
     3481 qkletteeik keslekvvlk pileelkqni vivqkhlame pessfkleav kniteaieel
     3541 yttatavqqv vaisseeaee mktskasale sfaksmeqle erclavinqq kadkiekpek
     3601 qqgklvdaqv lqkitdtihv lreslsqiee eklqeakelk itkeeeeagl klnvliepln
     3661 vlekalmtav eeskivteet tqelkkteia veklnlqpvl eelqksiavv qqgtsktavs
     3721 vdesdlmkma eksleemkls maavqelvsh qaqeikaeek leafaksmee agenllavak
     3781 qqelkkatlr ieeppalkvs edfvktvvtp iaelhttivk idketklske kekeeavifl
     3841 smvqplkkle esflsavqee ikqeaaeeir paipvlqklp iqstlkelnk nvaeiqeqlv
     3901 laketltrtg stedvavikt lerslsnlrt ssaivqqlaa ieepgqqild venvsalqaf
     3961 aiaveefkks caaiisrpav mssiigkvhl eeitkkeass tetilvpvki lqesictiee
     4021 lklqetgild tseikkpttv lseliaplqq leqsfmaaiq gehviehaae sfetdkasfe
     4081 ktalkpilee fqksiaivqe hmileegaqs lsdaeeaslk mmaqtvtdlk tsvaaiqqit
     4141 etvpemanel skgesvaafe tfakslhdlv eraatidhqq iilepaadti sedasslktw
     4201 adivedspvq alepmivdqg tiespseial siseeeaqvl kglakplsel reclavvvee
     4261 rksmdanema famseredls llktvaqpll elknaavsvi leqtavesag eksfeekpet
     4321 nlnpliesle elchsialie dqmlvesaed rtidevsllt alaeplmtlq rsvcvleerv
     4381 lspdveplpe esnwinecla vplqeieksi aeireccvve peeqsrmvtl dwsvveklve
     4441 pvevirstii gmedtvaeem kkctimknli qplsevhesf mllkdrtdlt vaeeeasida
     4501 ivdslsnlek sisfieeqta ekvedvsssa kddrlvigtl teslmqlkes imtveesptv
     4561 ylenleqplk elqsalesvs nirrekaeke ktkkivslee atrvstrlkn sikeindsie
     4621 sidnkiesfk gllgievqie nealkslakp leglkggiek ilekeevegs tvdslrklrn
     4681 aigiirqqsa dkplaesfhf etvdivgmlr pltpclhele tctekvetaw kensageglv
     4741 eletpisnlm dtinilrekl lrldivwvte eekkkkeark keeekaeklk qeeeehkkke
     4801 eaeklkqeee erkkkaeaer lqreeeerkk keeaerlkqe eeerkkkeet erlkqeeeer
     4861 qrkeeterlk qeeekrekke epeklkqeae ehrkkeeaer lkqeeeckkk eeaeklkqae
     4921 eegikreeee rkqeeeklrq egearkkkee eekikqeeet rkikekeekl kqeeeerkkk
     4981 edeeklrqee earkrkeerl kleeerkkke egeklqleqe rkkkeeedkl kqaeeerkkk
     5041 eeeerqwkek eeklkqeeet lkqeeearkk keeqerlkqq eekrkkkeee dnlkreeeer
     5101 knkekaeklk qkeeklkree eerkkkeeqd rlkqqeeerk kkeeeeklkl eeqerkkree
     5161 aeklkqeeel kkkqeaeklk qeeerkkkee aeklkaeeer kkkeeaeklk qeeerkkkee
     5221 aeklkaeeer kkkeeaeklk qeeerkkkee aeklkaeeer kkkeeaeklk qeeerkkkqe
     5281 aeklkaeeer kkkeeaeklk qkeerkkkee aeklkaeeer ekkeeaeklk qeeerkkkee
     5341 aeklkaekel kkeeeaeklk qeeerkkkee aeklkaeeer kkkeeaeklk qeeerkkkee
     5401 aeklkaekel kkeeeaeklk qeeerkkkee aeklkaeeer kkkeeaeklk qeeerkkkee
     5461 aeklkaeeel kkkdeavkqk edkkhkkkea ekleteekhk rkeeaekqke eedrkkkeae
     5521 qlkeqeerkk reeaeklkqe qenrkleees kkkkeeqerk qkeqaeklkq eeaeiskkae
     5581 sdtlkkkeek lkrkkeelkq qreederkek eeaekrrkeq eqrkkereer akkeeeerlk
     5641 reeeerkrke ekakqkkeee nrrkaeeaer lkkeqekeeq rreetkrrre eqekqirqet
     5701 ekirkaeeer lkkedetrer rrkerdlrrq eeeeklhkee eertkreeer rrrrreaerq
     5761 wredeeairr keterlerrr aedrqnreem erlkredeer rrrrdedrqt rheetvkalk
     5821 eehdrlrkrq eeetsrlrkr rqeqirddsw srmrqsesdq llkkmtddsf rlgtsktfss
     5881 dldfhrdksy kydsgfesal ssasrsyswr dsltsltrrr vddfidyklr dtsmdryyfd
     5941 tggyyrrrrk rddritrars isllkyddys tgdsdttitp aspilsrysr tktrsrndld
     6001 drgsnlslyl ppikdeitpr ekgkkpsfct rltnrtvgvg mrtrltctvl ghpeprvywt
     6061 kdgekletis nkyrirfdng mayfelheal pedsglytcv aenvhgiast esalkvypdf
     6121 qpalspptft rsikdtyrps dnelilecrv rghpiplisw lkdgcilqgd rykqcylddd
     6181 iyrleiaapn skdngrytcr amndlrteei shvvqfnerd rrvtskhekl yddhfsieaf
     6241 rrprfsnylt dysvptggtv alqvevkgvp apevrwfcge kkepvsipka ktftesgvht
     6301 lvlpevtese rgtymcrain ayghvdsiai vdvispsgsd sgkpamfvsr pvdksitvts
     6361 gedvsvsfrf sgvpkpkiiw mkglkditdg prshkevidd yvrltlkrvv psdegtycil
     6421 lknrygcdrs ffslkvkqra rsltpdwstl ssrtetgslh gissesrdde msyvknvpgp
     6481 issepvvvdg gknwlslswg kaerrgpapv iayrvdawll gsdggarwve lgmtpinafd
     6541 afnlkpggey kfqvtprnry gwgesvtmtn svrvsetvdl peftkilpgq lkilegttvk
     6601 leceirvdsh vdvkwyrest eidprysify ngsrcsltie kvqesdngry vceasnlvgk
     6661 assfarvlvv ddpkiieada klksrllgdd tedrppeftm rirdrrvqat ypvrltcqvt
     6721 gqpvpvvtwy kndteirqde rhifwdddsn fhtleithst ledsgcymvt aknangsvsc
     6781 rcilvvdkgi rayiapeflc gldaaytvkl ggdlrmtaqi eaypsvgvvw hrdgnrlrps
     6841 rrtvmtlnhd gtvelslakv tardagvycc tatnevghae tstrvsiigt etegeqtsld
     6901 gvptvtlass dipyskeplf vtkplsteai egdaviisce vvgdpkpevm wlrdflkpdy
     6961 yrdaaqfrlv gegpqyrlei pyakldftgt ysviarnchg eakavislqi yakgqgkeek
     7021 mkksgvthgn vltlpvitre lrdlrccdgd avtleckvqa tpeaplvrwe rggkilqisg
     7081 dfssefdget arlsiqhvyp edegeytcva yndlgkafts aclivdvpeg kenvlcqrlt
     7141 rpagllsags tprstprstp irslspavsh grelrspqll prsrsmlrrp kisppkfyav
     7201 phnrvveege tvrfqcavvg hptpwvkwdk nglavtpttr isikerddvk ileivevtqe
     7261 daglyrviae ndygrieasa rleiinrqll gpvsrtirtr sasprtypsf grslldttsr
     7321 ingrlyldcs irgtpsptpt wfrngrpler snrikryfdg ktakveiskv kasdageytc
     7381 vatnvlgstk ntcqvivldp hdpstsdkna pkflqslpde sivmeghcye lqtrltgtpp
     7441 fsvtwlkndr eipdndccky iiygdggial rlsnvcseda geytclvrnn fgeascnglf
     7501 avqdykgvpk lapqftktpl svitskgeta cfcarvqcgk pmeiawting kdvkenprck
     7561 ieqddnvsil riqdvsardv gdirctasvi gkgpsiscta qlqlnrpvrn iedsaskcnn
     7621 aqaeirngrp ppkpgnftrn nslrktardv pslsqnqrne spmrsrsssl plrtspqrss
     7681 paptrrntps plpdtrkase nklmkkernf kkleriqkde kvplsskdee atnkppddkp
     7741 rlknseddee kcsmslnsdf sktengrkrs qdngnkhddt piicmecave epdeaskkdq
     7801 etvtkaekvd svrkeflaas iikvpadvtv ftgnrvvlrv tyrghpeprv rwfragrelt
     7861 pnkktaityg ggvsclisdd vtadnagkye vsvenefgkd rrcfsvaveg ppdppagips
     7921 vccspgtvti nwrsspydgg ctvtgytvem shpgentwtr iaesclslsl tapasgtnap
     7981 ipgekyrfrv raenihgvse pgdesefvri pkegemclqd deeefeaafe arlvemedgq
     8041 lfnnryevle elgkgrygvv krvverssst sfaakfvrti kskdkeqvre eikimnmlrh
     8101 pkllllaaay espreivmvt eyisggelfe rvvaddftlt erdsilfmrq icegveymhq
     8161 nnvvhldlkp enimcrtrts hqiklidfgl aqtlkpdtpi rvlfgtpefi ppeiisyepi
     8221 gtesdmwsvg vicyvlltgl spfmgdndae tfanitrady dledqafdai sndakdfisg
     8281 llvkrkelrm sarqclehpw maqhaeamsr ialpteklkk fivrrkwqkt gnairalgrm
     8341 ailsansrrs ptgtaesspt serpnpidsp rpinfdvspt teskkhdidd rtekkkstge
     8401 sdvqernvys fcyktevnil peetldsser eearvssdld easlknepaw drvrscsrgn
     8461 gseeiqetdt seeaqkpdea hleepvpmek ieeeteeiit rcryeeriti keetlfvqnf
     8521 eperntlnie systsmtscn eedysnnise evtssinket dvpeankvkt irkieekrid
     8581 eeisetekvs kekidqklrr vfrgdsrdsg igdcisnlss ssqqvnelgi ssikeeetdn
     8641 dnnngnarsv skkveqfenr kifvekmtdn emtqensdvs dpkkctkgsi evdtlsdvtk
     8701 inrqheavne iedprlligr vrskfvptgn vsrtaklfek eataskspan vpqvpqrtyp
     8761 avpvvagkpq neriqkafaf wnk
//
LOCUS       XP_076546016            1426 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305236 [Osmia lignaria lignaria].
ACCESSION   XP_076546016
VERSION     XP_076546016.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689901.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1426
                     /product="uncharacterized protein LOC143305236"
                     /calculated_mol_wt=160894
     Region          32..129
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(32,102,117)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(118..119,121..122)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          140..227
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          157..161
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          170..174
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          195..199
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          209..214
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          222..225
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          251..325
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          253..257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          266..270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          291..295
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          305..310
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          318..321
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          329..419
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(329,393,408)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(409..410,412..413)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          <441..659
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..1426
                     /gene="LOC143305236"
                     /coded_by="XM_076689901.1:514..4794"
                     /db_xref="GeneID:143305236"
ORIGIN      
        1 mgnataksry sksslqvggt nrrprwegsg lpappgkpil ipgadesqpd vvairwersp
       61 snggsailgy lvehrrlgsq hwvrstptlc tfpeltlsgl epgwryqfrv raqnavglsr
      121 pseisdaltv tlqrsassap yfeqelkdtv vleneqaefa vrftgsplpk iswfkdgfei
      181 fssrrtriit dsgrsvllih qtalndegei kctatnragh tstkarlvle appkirlprq
      241 yedgllfeqd etirlkvsla grpspsviwy hdgeiiahdq rhifetmdge svlkipdakr
      301 vdrgeytvka vnklgedtss flvtvtdrpa apgkamvtmt lgrtvslswr epeddggcki
      361 gtyiveyyri gwdvwlkatt srqtkatlse liegseykfr vkaenpygvs epseesdvif
      421 ipdlkrgimt pslsgksqsh reirsrekre vsfavpaqrt rsltreesrn rdedddlrfg
      481 pgnrsssaqr ltgrpsrads rvtfaldtld nseapippar srdhstlkhe nrvenhsdrp
      541 svpvaapvnq skekptrvtv spppamdess rraifresrs rsvsiprers msplsmpkiq
      601 eqreeegslt ssrlrpslnl ilkkqqatvd epqpplvksn sldvdkpgsp ttpreddesv
      661 lhgssefmlv lypedqdqgk kyrdidgrrk tsdtrqaaee tedmedlipp pmslslpelf
      721 slehqvvetl reavsstell heramerfyk avaaeeasev gkrkiqlerk tgelastvet
      781 kaediedtqd rllslrrrms npavvpqnli twqpkknrrr sseadtmkts iklvtpslla
      841 diearsdpnl pndtetpvah pwaldkeeds atqlrrwhda nvplverine dksipwqsst
      901 nqesvsnrgv vpeslpilqq dtkqeemeee eeeesietse esseemssad sedlkllkar
      961 ilarqvleee dtyhpsgrpi lhiepepppi pphrvpilev spptptrtva pplsptnlvp
     1021 ksilkkpkee lapqnsfgrp vppekpirki ppqaaevakd eeiqpqvaae mirppvmses
     1081 dtdsmlsage aaknrriqak mrsnvseeev deddiearma vvdhyteivr ehsskfnyrs
     1141 seerklgdsv assrrssfse dqeqshlkdr leafatksnr qetreaqqvq lpkvkskkne
     1201 qvgqakrisg srgttpardt kpgekrisrp ssrnqspapr srnvsvergg assrsssktr
     1261 vrapsqdrrp psrtgsedrr yvreqsvdeg vrrtkrassr trsrssskdr nrpetpielk
     1321 kerlqrslds kkyrpsrrrf pagypevpwr esrlneerla ieakynvrnt vgyvtdltll
     1381 laavyvylfk ketlaipfia lllyrrvqhe irgkisggww wskrkq
//
LOCUS       XP_034183109            1029 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 4 kinase [Osmia lignaria lignaria].
ACCESSION   XP_034183109
VERSION     XP_034183109.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327218.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1029
                     /product="pre-mRNA processing factor 4 kinase"
                     /calculated_mol_wt=120712
     Region          <208..>668
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          218..>436
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          709..1025
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,754,774,790..793,796,798..799,
                     838,840,842..843,845,857,860,870,872..875,877,914)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,774,790..793,796,838,840,
                     842..843,845,857)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(754,798,838,840,860,870,872..875,877,914)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            856..877
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..1029
                     /gene="Prp4k"
                     /coded_by="XM_034327218.2:155..3244"
                     /db_xref="GeneID:117605656"
ORIGIN      
        1 mgtsdlecle skrdivgvds esdmlidqrk kkkrkhkhhk hkkdkliere dgrierqerk
       61 rhkkhkrpkk erevengsld dkssrviqkd igvngkvkns llevvstees edeklvdlds
      121 devdctiied didleelmkq kerlqaclvq ylsdesekee kepdqdevkv aetpdvilve
      181 ddsendnipp kkrarsksgs rerkkivkpe rrvvvdmtrd rrsreehkek rrdsdrrrds
      241 dkvrtkeevr redsrkddrq vrkpsdrhrd dsrkednkrr seddkrkdpv krdesrkreq
      301 dkreedrrrd sdhhnnsrsr tehksldksd ksrrdsrdrl enrerqssrd rhasrdrhvs
      361 rdrhgsrdrh asrdrhasrd rhasrdrpas rdrrsrdrpp srdrhsrdkr dsrerhtsre
      421 rhrdsrgsrd rndvrrdsrn herirdrsrs prrsrsplrs rldrdrndry khsrslsrsr
      481 rdrdkhardh drdrekngkr ergdkykdsl seglkadrsd tsseedikdi dieeeedeea
      541 iierrrkqre allkrlggpn edsnmsadin tvpatppses qsnvsqksie ipsnnnests
      601 eshtpplpek pksplikprk srfedppsee adkiedtkpa ekvkqddkqn akkanewdmf
      661 aeadnigdfn sptvegkrqg gpdnpsltdn wddaegyyrv rvgetldsry vvygytgqgv
      721 fsnvvrards argnldvavk iirnneimhk tglkeleilr klndadpedr fhclrlfrhf
      781 yhknhlcmvf eplamnlrev lkkygkdvgl hvkavrsytq qlflalkllk ranilhadik
      841 pdnilvsesk lvlklcdfgs ashaheneia pylvsrfyra peiilgtpyd fgidmwsvgc
      901 tiyelytgki mfsgktnnqm lkffmdlkgk ipnklirkgt fkdlhfdsnc nflyheidkv
      961 terakvvvms tipatrdlga elggnslppe qsrkvgqlkd llertlmlda gkritvnhal
     1021 ahpfiqeki
//
LOCUS       XP_034183126            1029 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 4 kinase [Osmia lignaria lignaria].
ACCESSION   XP_034183126
VERSION     XP_034183126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1029
                     /product="pre-mRNA processing factor 4 kinase"
                     /calculated_mol_wt=120712
     Region          <208..>668
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          218..>436
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          709..1025
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,754,774,790..793,796,798..799,
                     838,840,842..843,845,857,860,870,872..875,877,914)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,774,790..793,796,838,840,
                     842..843,845,857)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(754,798,838,840,860,870,872..875,877,914)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            856..877
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..1029
                     /gene="Prp4k"
                     /coded_by="XM_034327235.2:155..3244"
                     /db_xref="GeneID:117605656"
ORIGIN      
        1 mgtsdlecle skrdivgvds esdmlidqrk kkkrkhkhhk hkkdkliere dgrierqerk
       61 rhkkhkrpkk erevengsld dkssrviqkd igvngkvkns llevvstees edeklvdlds
      121 devdctiied didleelmkq kerlqaclvq ylsdesekee kepdqdevkv aetpdvilve
      181 ddsendnipp kkrarsksgs rerkkivkpe rrvvvdmtrd rrsreehkek rrdsdrrrds
      241 dkvrtkeevr redsrkddrq vrkpsdrhrd dsrkednkrr seddkrkdpv krdesrkreq
      301 dkreedrrrd sdhhnnsrsr tehksldksd ksrrdsrdrl enrerqssrd rhasrdrhvs
      361 rdrhgsrdrh asrdrhasrd rhasrdrpas rdrrsrdrpp srdrhsrdkr dsrerhtsre
      421 rhrdsrgsrd rndvrrdsrn herirdrsrs prrsrsplrs rldrdrndry khsrslsrsr
      481 rdrdkhardh drdrekngkr ergdkykdsl seglkadrsd tsseedikdi dieeeedeea
      541 iierrrkqre allkrlggpn edsnmsadin tvpatppses qsnvsqksie ipsnnnests
      601 eshtpplpek pksplikprk srfedppsee adkiedtkpa ekvkqddkqn akkanewdmf
      661 aeadnigdfn sptvegkrqg gpdnpsltdn wddaegyyrv rvgetldsry vvygytgqgv
      721 fsnvvrards argnldvavk iirnneimhk tglkeleilr klndadpedr fhclrlfrhf
      781 yhknhlcmvf eplamnlrev lkkygkdvgl hvkavrsytq qlflalkllk ranilhadik
      841 pdnilvsesk lvlklcdfgs ashaheneia pylvsrfyra peiilgtpyd fgidmwsvgc
      901 tiyelytgki mfsgktnnqm lkffmdlkgk ipnklirkgt fkdlhfdsnc nflyheidkv
      961 terakvvvms tipatrdlga elggnslppe qsrkvgqlkd llertlmlda gkritvnhal
     1021 ahpfiqeki
//
LOCUS       XP_034183135            1029 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 4 kinase [Osmia lignaria lignaria].
ACCESSION   XP_034183135
VERSION     XP_034183135.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327244.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1029
                     /product="pre-mRNA processing factor 4 kinase"
                     /calculated_mol_wt=120712
     Region          <208..>668
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          218..>436
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          709..1025
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,754,774,790..793,796,798..799,
                     838,840,842..843,845,857,860,870,872..875,877,914)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,774,790..793,796,838,840,
                     842..843,845,857)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(754,798,838,840,860,870,872..875,877,914)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            856..877
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..1029
                     /gene="Prp4k"
                     /coded_by="XM_034327244.2:155..3244"
                     /db_xref="GeneID:117605656"
ORIGIN      
        1 mgtsdlecle skrdivgvds esdmlidqrk kkkrkhkhhk hkkdkliere dgrierqerk
       61 rhkkhkrpkk erevengsld dkssrviqkd igvngkvkns llevvstees edeklvdlds
      121 devdctiied didleelmkq kerlqaclvq ylsdesekee kepdqdevkv aetpdvilve
      181 ddsendnipp kkrarsksgs rerkkivkpe rrvvvdmtrd rrsreehkek rrdsdrrrds
      241 dkvrtkeevr redsrkddrq vrkpsdrhrd dsrkednkrr seddkrkdpv krdesrkreq
      301 dkreedrrrd sdhhnnsrsr tehksldksd ksrrdsrdrl enrerqssrd rhasrdrhvs
      361 rdrhgsrdrh asrdrhasrd rhasrdrpas rdrrsrdrpp srdrhsrdkr dsrerhtsre
      421 rhrdsrgsrd rndvrrdsrn herirdrsrs prrsrsplrs rldrdrndry khsrslsrsr
      481 rdrdkhardh drdrekngkr ergdkykdsl seglkadrsd tsseedikdi dieeeedeea
      541 iierrrkqre allkrlggpn edsnmsadin tvpatppses qsnvsqksie ipsnnnests
      601 eshtpplpek pksplikprk srfedppsee adkiedtkpa ekvkqddkqn akkanewdmf
      661 aeadnigdfn sptvegkrqg gpdnpsltdn wddaegyyrv rvgetldsry vvygytgqgv
      721 fsnvvrards argnldvavk iirnneimhk tglkeleilr klndadpedr fhclrlfrhf
      781 yhknhlcmvf eplamnlrev lkkygkdvgl hvkavrsytq qlflalkllk ranilhadik
      841 pdnilvsesk lvlklcdfgs ashaheneia pylvsrfyra peiilgtpyd fgidmwsvgc
      901 tiyelytgki mfsgktnnqm lkffmdlkgk ipnklirkgt fkdlhfdsnc nflyheidkv
      961 terakvvvms tipatrdlga elggnslppe qsrkvgqlkd llertlmlda gkritvnhal
     1021 ahpfiqeki
//
LOCUS       XP_034183144            1029 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 4 kinase [Osmia lignaria lignaria].
ACCESSION   XP_034183144
VERSION     XP_034183144.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327253.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1029
                     /product="pre-mRNA processing factor 4 kinase"
                     /calculated_mol_wt=120712
     Region          <208..>668
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          218..>436
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          709..1025
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,754,774,790..793,796,798..799,
                     838,840,842..843,845,857,860,870,872..875,877,914)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,774,790..793,796,838,840,
                     842..843,845,857)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(754,798,838,840,860,870,872..875,877,914)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            856..877
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..1029
                     /gene="Prp4k"
                     /coded_by="XM_034327253.2:155..3244"
                     /db_xref="GeneID:117605656"
ORIGIN      
        1 mgtsdlecle skrdivgvds esdmlidqrk kkkrkhkhhk hkkdkliere dgrierqerk
       61 rhkkhkrpkk erevengsld dkssrviqkd igvngkvkns llevvstees edeklvdlds
      121 devdctiied didleelmkq kerlqaclvq ylsdesekee kepdqdevkv aetpdvilve
      181 ddsendnipp kkrarsksgs rerkkivkpe rrvvvdmtrd rrsreehkek rrdsdrrrds
      241 dkvrtkeevr redsrkddrq vrkpsdrhrd dsrkednkrr seddkrkdpv krdesrkreq
      301 dkreedrrrd sdhhnnsrsr tehksldksd ksrrdsrdrl enrerqssrd rhasrdrhvs
      361 rdrhgsrdrh asrdrhasrd rhasrdrpas rdrrsrdrpp srdrhsrdkr dsrerhtsre
      421 rhrdsrgsrd rndvrrdsrn herirdrsrs prrsrsplrs rldrdrndry khsrslsrsr
      481 rdrdkhardh drdrekngkr ergdkykdsl seglkadrsd tsseedikdi dieeeedeea
      541 iierrrkqre allkrlggpn edsnmsadin tvpatppses qsnvsqksie ipsnnnests
      601 eshtpplpek pksplikprk srfedppsee adkiedtkpa ekvkqddkqn akkanewdmf
      661 aeadnigdfn sptvegkrqg gpdnpsltdn wddaegyyrv rvgetldsry vvygytgqgv
      721 fsnvvrards argnldvavk iirnneimhk tglkeleilr klndadpedr fhclrlfrhf
      781 yhknhlcmvf eplamnlrev lkkygkdvgl hvkavrsytq qlflalkllk ranilhadik
      841 pdnilvsesk lvlklcdfgs ashaheneia pylvsrfyra peiilgtpyd fgidmwsvgc
      901 tiyelytgki mfsgktnnqm lkffmdlkgk ipnklirkgt fkdlhfdsnc nflyheidkv
      961 terakvvvms tipatrdlga elggnslppe qsrkvgqlkd llertlmlda gkritvnhal
     1021 ahpfiqeki
//
LOCUS       XP_034183194            1029 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 4 kinase [Osmia lignaria lignaria].
ACCESSION   XP_034183194
VERSION     XP_034183194.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327303.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1029
                     /product="pre-mRNA processing factor 4 kinase"
                     /calculated_mol_wt=120712
     Region          <208..>668
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          218..>436
                     /region_name="PRK12678"
                     /note="transcription termination factor Rho; Provisional"
                     /db_xref="CDD:237171"
     Region          709..1025
                     /region_name="STKc_PRP4"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Pre-mRNA-Processing factor 4; cd14135"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,754,774,790..793,796,798..799,
                     838,840,842..843,845,857,860,870,872..875,877,914)
                     /site_type="active"
                     /db_xref="CDD:271037"
     Site            order(716..720,724,738,740,774,790..793,796,838,840,
                     842..843,845,857)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271037"
     Site            order(754,798,838,840,860,870,872..875,877,914)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271037"
     Site            856..877
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271037"
     CDS             1..1029
                     /gene="Prp4k"
                     /coded_by="XM_034327303.2:155..3244"
                     /db_xref="GeneID:117605656"
ORIGIN      
        1 mgtsdlecle skrdivgvds esdmlidqrk kkkrkhkhhk hkkdkliere dgrierqerk
       61 rhkkhkrpkk erevengsld dkssrviqkd igvngkvkns llevvstees edeklvdlds
      121 devdctiied didleelmkq kerlqaclvq ylsdesekee kepdqdevkv aetpdvilve
      181 ddsendnipp kkrarsksgs rerkkivkpe rrvvvdmtrd rrsreehkek rrdsdrrrds
      241 dkvrtkeevr redsrkddrq vrkpsdrhrd dsrkednkrr seddkrkdpv krdesrkreq
      301 dkreedrrrd sdhhnnsrsr tehksldksd ksrrdsrdrl enrerqssrd rhasrdrhvs
      361 rdrhgsrdrh asrdrhasrd rhasrdrpas rdrrsrdrpp srdrhsrdkr dsrerhtsre
      421 rhrdsrgsrd rndvrrdsrn herirdrsrs prrsrsplrs rldrdrndry khsrslsrsr
      481 rdrdkhardh drdrekngkr ergdkykdsl seglkadrsd tsseedikdi dieeeedeea
      541 iierrrkqre allkrlggpn edsnmsadin tvpatppses qsnvsqksie ipsnnnests
      601 eshtpplpek pksplikprk srfedppsee adkiedtkpa ekvkqddkqn akkanewdmf
      661 aeadnigdfn sptvegkrqg gpdnpsltdn wddaegyyrv rvgetldsry vvygytgqgv
      721 fsnvvrards argnldvavk iirnneimhk tglkeleilr klndadpedr fhclrlfrhf
      781 yhknhlcmvf eplamnlrev lkkygkdvgl hvkavrsytq qlflalkllk ranilhadik
      841 pdnilvsesk lvlklcdfgs ashaheneia pylvsrfyra peiilgtpyd fgidmwsvgc
      901 tiyelytgki mfsgktnnqm lkffmdlkgk ipnklirkgt fkdlhfdsnc nflyheidkv
      961 terakvvvms tipatrdlga elggnslppe qsrkvgqlkd llertlmlda gkritvnhal
     1021 ahpfiqeki
//
LOCUS       XP_034183260             555 aa            linear   INV 03-SEP-2025
DEFINITION  esterase FE4 [Osmia lignaria lignaria].
ACCESSION   XP_034183260
VERSION     XP_034183260.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327369.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183260.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..555
                     /product="esterase FE4"
                     /calculated_mol_wt=61669
     Region          34..548
                     /region_name="COesterase"
                     /note="Carboxylesterase family; pfam00135"
                     /db_xref="CDD:395084"
     Site            order(138..140,218..220,223,380,384..385,415,468,471)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(219,348,467)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..555
                     /gene="LOC117605721"
                     /coded_by="XM_034327369.2:34..1701"
                     /db_xref="GeneID:117605721"
ORIGIN      
        1 miavrnrfaf dvhslcrirw svvklvkvaf tmneepivsv kegklrggiv kgvletsyia
       61 fkgipfaapp vgelrfkdpq ppapwsgikd tsesigysct qleelppynv igkedclyln
      121 vytnslnqsk pvmfwihgga yivgsgsyki trpdylltkd vvlvtanyrl gafgflnlgh
      181 svapgnqglk dlimalkwvk enianfggdp dnvtifgpsa ggalthflll sprarglfhk
      241 aimqsglvtc swttnqsrpe rgfklasilg kdstdpievv eflrkipaad iakstalilt
      301 teethsfhlp fapntdeiae dpvlplpide llstdvdipv magytshefi mffkdksqaa
      361 mniynqflpq hvknlaalrn lgpeeteell ktvkekyfnn epigednidk lvefigdvyf
      421 gypaklyaed rvkrtsapty lyrysyvgne ktatdllvkr lvtgashvde vaylfylplc
      481 ktenpdppap gsedrttmer mttmwsnfag tsnptpvhdd ciqttwepvt kdklcyldig
      541 delqlitfss hilns
//
LOCUS       XP_034183359             449 aa            linear   INV 03-SEP-2025
DEFINITION  integrin linked kinase [Osmia lignaria lignaria].
ACCESSION   XP_034183359
VERSION     XP_034183359.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327468.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="integrin linked kinase"
                     /calculated_mol_wt=50421
     Region          <8..192
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(33,35,39..40,43..45,47..48,52,55,64,66,68,72..73,
                     76..78,80..81,85,88,97,99,101,105..106,109..111,113..114,
                     118,121,130)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          33..64
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          66..97
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          99..130
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          196..447
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(198..201,204,206,217,219,250,266..269,317,321..322,
                     324,334..335)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..449
                     /gene="Ilk"
                     /coded_by="XM_034327468.2:367..1716"
                     /db_xref="GeneID:117605768"
ORIGIN      
        1 medifqwcre gnamqvrvwl ddtehdmnqg ddhgfsplhw cckeghlkla ellvsrgari
       61 natnrgddtp lhlasahghk eivqlllrnr advnvtnehg ntalhyacfw gdqavaeelv
      121 aagalvsian kdgdtpldka rghlakrlhd laveygqdlk kiqfkdqswl glktrsrdat
      181 lsrhkginma dlslhthlas tpsgetwrgr wqnndivaki lnirectari srdfneefpk
      241 lrifshpnvl pvlgcvnqpp qlatvsqyma rgslhrllhg gtgvvvdtar alrlaldvar
      301 amaflhgler qnrcrfqlns khimidedlt arvnmadskf sfqevgriye pawmspeals
      361 krpadinlea sdmwsfavll welatrevpf adlspmecgm kialedlrvs ippgisphla
      421 kliricmned pgkrpsfdmv vpildkmkr
//
LOCUS       XP_034183474             272 aa            linear   INV 03-SEP-2025
DEFINITION  inositol monophosphatase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034183474
VERSION     XP_034183474.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327583.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="inositol monophosphatase 1"
                     /calculated_mol_wt=29717
     Region          7..252
                     /region_name="IMPase"
                     /note="inositol monophosphatase and related domains. A
                     family of Mg++ dependent phosphatases, inhibited by
                     lithium, many of which may act on inositol monophosphate
                     substrate. They dephosphorylate inositol phosphate to
                     generate inositol, which may be recycled...; cd01639"
                     /db_xref="CDD:238817"
     Site            order(35,40,46,69..70,89..94,217..218)
                     /site_type="active"
                     /db_xref="CDD:238817"
     Site            order(39,41,95..96,98..103,155..157,159,161..162,171..172,
                     175,178..179,182,186..192,200,204..207)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238817"
     CDS             1..272
                     /gene="LOC117605832"
                     /coded_by="XM_034327583.2:171..989"
                     /db_xref="GeneID:117605832"
ORIGIN      
        1 msnineyyla vlrlvkeags ivrekinqpk damikscevd lvtewdqkve klliegissk
       61 fpdhkfigee esalgnkiel tdaptwiidp idgtmnfvhg lphtcisial linktpeigi
      121 vynpileqlf tacrgqgafl ngapiqvsge kelrkalvmm emgtsrdpek mkivlenann
      181 ltsqvhgirs lgsaalnmcm valggadisf eygihawdva agdiivreag gvcidpaggp
      241 fdvmnrrvlc astmelaqel akvliqyype rd
//
LOCUS       XP_076546018             635 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546018
VERSION     XP_076546018.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689903.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..635
                     /product="soluble guanylate cyclase 89Da isoform X1"
                     /calculated_mol_wt=72006
     Region          2..172
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          214..424
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          435..611
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(444,446..450,485,487..489,492,554..556,560..561,
                     564..565,601)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(446,489)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(462,465..466,469,473,485..486,542,545,555..558,561,
                     601)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..635
                     /gene="LOC117611006"
                     /coded_by="XM_076689903.1:713..2620"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 mygmllesvq hfvqvgnvty ksleygedvw lqivekaeck hivfntrqty pdelmtnlaa
       61 alvafngetv dnvmqffgkc fvrffsnlgy acmikatgry fcdflqsvdn ihmqmrftyp
      121 kmkspsmyit hidtegvilv yrstrkgfth ylmgqlfqia kelyeidlni kvlgssnnip
      181 gsrsvmvkfr idfdnreyia knnrmkvpls relppvsctf ilrlfpfgvv inkdmqilga
      241 gdkllqawgg sssirdkhit evfklrrpkg isftwgnvmy lhsvmfelel lrlnddmsss
      301 sniastssgl drrgsqgvrs illkgqmryi edidaiiflc splinsldel lnmglylndl
      361 nphglsrelv lagwqhcgrl emmferaeqr stelensyal ldrwknksde llysmipqtv
      421 adrlragvsp lstcesfesi tvlfcelcdf dystiegamd ivssmnavfs cfdslmdefn
      481 vykvetvgrv ymaasgapdr tehhaqniad fslqllkhvr slklpsgldi qirigihsgp
      541 avagvvgikv prycffgdtv ntasrmqtts epgrihisad tktllpqdry hvesrglvwv
      601 kgkgnmetyw lsdkqktnii sveviqpkrl litdi
//
LOCUS       XP_076546019             627 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546019
VERSION     XP_076546019.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689904.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="soluble guanylate cyclase 89Da isoform X2"
                     /calculated_mol_wt=71158
     Region          2..164
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          206..416
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          427..603
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(436,438..442,477,479..481,484,546..548,552..553,
                     556..557,593)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(438,481)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(454,457..458,461,465,477..478,534,537,547..550,553,
                     593)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..627
                     /gene="LOC117611006"
                     /coded_by="XM_076689904.1:711..2594"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 mygmllesvq hfvqleyged vwlqivekae ckhivfntrq typdelmtnl aaalvafnge
       61 tvdnvmqffg kcfvrffsnl gyacmikatg ryfcdflqsv dnihmqmrft ypkmkspsmy
      121 ithidtegvi lvyrstrkgf thylmgqlfq iakelyeidl nikvlgssnn ipgsrsvmvk
      181 fridfdnrey iaknnrmkvp lsrelppvsc tfilrlfpfg vvinkdmqil gagdkllqaw
      241 ggsssirdkh itevfklrrp kgisftwgnv mylhsvmfel ellrlnddms sssniastss
      301 gldrrgsqgv rsillkgqmr yiedidaiif lcsplinsld ellnmglyln dlnphglsre
      361 lvlagwqhcg rlemmferae qrstelensy alldrwknks dellysmipq tvadrlragv
      421 splstcesfe sitvlfcelc dfdystiega mdivssmnav fscfdslmde fnvykvetvg
      481 rvymaasgap drtehhaqni adfslqllkh vrslklpsgl diqirigihs gpavagvvgi
      541 kvprycffgd tvntasrmqt tsepgrihis adtktllpqd ryhvesrglv wvkgkgnmet
      601 ywlsdkqktn iisveviqpk rllitdi
//
LOCUS       XP_034194780             622 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194780
VERSION     XP_034194780.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338889.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..622
                     /product="soluble guanylate cyclase 89Da isoform X3"
                     /calculated_mol_wt=70654
     Region          11..159
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          201..411
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          422..598
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(431,433..437,472,474..476,479,541..543,547..548,
                     551..552,588)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(433,476)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(449,452..453,456,460,472..473,529,532,542..545,548,
                     588)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..622
                     /gene="LOC117611006"
                     /coded_by="XM_034338889.2:242..2110"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 myitflveyl eygedvwlqi vekaeckhiv fntrqtypde lmtnlaaalv afngetvdnv
       61 mqffgkcfvr ffsnlgyacm ikatgryfcd flqsvdnihm qmrftypkmk spsmyithid
      121 tegvilvyrs trkgfthylm gqlfqiakel yeidlnikvl gssnnipgsr svmvkfridf
      181 dnreyiaknn rmkvplsrel ppvsctfilr lfpfgvvink dmqilgagdk llqawggsss
      241 irdkhitevf klrrpkgisf twgnvmylhs vmfelellrl nddmssssni astssgldrr
      301 gsqgvrsill kgqmryiedi daiiflcspl insldellnm glylndlnph glsrelvlag
      361 wqhcgrlemm feraeqrste lensyalldr wknksdelly smipqtvadr lragvsplst
      421 cesfesitvl fcelcdfdys tiegamdivs smnavfscfd slmdefnvyk vetvgrvyma
      481 asgapdrteh haqniadfsl qllkhvrslk lpsgldiqir igihsgpava gvvgikvpry
      541 cffgdtvnta srmqttsepg rihisadtkt llpqdryhve srglvwvkgk gnmetywlsd
      601 kqktniisve viqpkrllit di
//
LOCUS       XP_034194782             581 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194782
VERSION     XP_034194782.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338891.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="soluble guanylate cyclase 89Da isoform X4"
                     /calculated_mol_wt=65644
     Region          <1..118
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          160..370
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          381..557
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(390,392..396,431,433..435,438,500..502,506..507,
                     510..511,547)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(392,435)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(408,411..412,415,419,431..432,488,491,501..504,507,
                     547)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..581
                     /gene="LOC117611006"
                     /coded_by="XM_034338891.2:441..2186"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 mtnlaaalva fngetvdnvm qffgkcfvrf fsnlgyacmi katgryfcdf lqsvdnihmq
       61 mrftypkmks psmyithidt egvilvyrst rkgfthylmg qlfqiakely eidlnikvlg
      121 ssnnipgsrs vmvkfridfd nreyiaknnr mkvplsrelp pvsctfilrl fpfgvvinkd
      181 mqilgagdkl lqawggsssi rdkhitevfk lrrpkgisft wgnvmylhsv mfelellrln
      241 ddmssssnia stssgldrrg sqgvrsillk gqmryiedid aiiflcspli nsldellnmg
      301 lylndlnphg lsrelvlagw qhcgrlemmf eraeqrstel ensyalldrw knksdellys
      361 mipqtvadrl ragvsplstc esfesitvlf celcdfdyst iegamdivss mnavfscfds
      421 lmdefnvykv etvgrvymaa sgapdrtehh aqniadfslq llkhvrslkl psgldiqiri
      481 gihsgpavag vvgikvpryc ffgdtvntas rmqttsepgr ihisadtktl lpqdryhves
      541 rglvwvkgkg nmetywlsdk qktniisvev iqpkrllitd i
//
LOCUS       XP_034194781             581 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194781
VERSION     XP_034194781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="soluble guanylate cyclase 89Da isoform X4"
                     /calculated_mol_wt=65644
     Region          <1..118
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          160..370
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          381..557
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(390,392..396,431,433..435,438,500..502,506..507,
                     510..511,547)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(392,435)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(408,411..412,415,419,431..432,488,491,501..504,507,
                     547)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..581
                     /gene="LOC117611006"
                     /coded_by="XM_034338890.2:171..1916"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 mtnlaaalva fngetvdnvm qffgkcfvrf fsnlgyacmi katgryfcdf lqsvdnihmq
       61 mrftypkmks psmyithidt egvilvyrst rkgfthylmg qlfqiakely eidlnikvlg
      121 ssnnipgsrs vmvkfridfd nreyiaknnr mkvplsrelp pvsctfilrl fpfgvvinkd
      181 mqilgagdkl lqawggsssi rdkhitevfk lrrpkgisft wgnvmylhsv mfelellrln
      241 ddmssssnia stssgldrrg sqgvrsillk gqmryiedid aiiflcspli nsldellnmg
      301 lylndlnphg lsrelvlagw qhcgrlemmf eraeqrstel ensyalldrw knksdellys
      361 mipqtvadrl ragvsplstc esfesitvlf celcdfdyst iegamdivss mnavfscfds
      421 lmdefnvykv etvgrvymaa sgapdrtehh aqniadfslq llkhvrslkl psgldiqiri
      481 gihsgpavag vvgikvpryc ffgdtvntas rmqttsepgr ihisadtktl lpqdryhves
      541 rglvwvkgkg nmetywlsdk qktniisvev iqpkrllitd i
//
LOCUS       XP_076546020             576 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546020
VERSION     XP_076546020.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689905.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..576
                     /product="soluble guanylate cyclase 89Da isoform X5"
                     /calculated_mol_wt=65467
     Region          <31..113
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          155..365
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          376..552
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(385,387..391,426,428..430,433,495..497,501..502,
                     505..506,542)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(387,430)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(403,406..407,410,414,426..427,483,486,496..499,502,
                     542)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..576
                     /gene="LOC117611006"
                     /coded_by="XM_076689905.1:665..2395"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 mytsfcevip rkqleqcmac ywrvystscr yacmikatgr yfcdflqsvd nihmqmrfty
       61 pkmkspsmyi thidtegvil vyrstrkgft hylmgqlfqi akelyeidln ikvlgssnni
      121 pgsrsvmvkf ridfdnreyi aknnrmkvpl srelppvsct filrlfpfgv vinkdmqilg
      181 agdkllqawg gsssirdkhi tevfklrrpk gisftwgnvm ylhsvmfele llrlnddmss
      241 ssniastssg ldrrgsqgvr sillkgqmry iedidaiifl csplinslde llnmglylnd
      301 lnphglsrel vlagwqhcgr lemmferaeq rstelensya lldrwknksd ellysmipqt
      361 vadrlragvs plstcesfes itvlfcelcd fdystiegam divssmnavf scfdslmdef
      421 nvykvetvgr vymaasgapd rtehhaqnia dfslqllkhv rslklpsgld iqirigihsg
      481 pavagvvgik vprycffgdt vntasrmqtt sepgrihisa dtktllpqdr yhvesrglvw
      541 vkgkgnmety wlsdkqktni isveviqpkr llitdi
//
LOCUS       XP_034194785             543 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194785
VERSION     XP_034194785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..543
                     /product="soluble guanylate cyclase 89Da isoform X6"
                     /calculated_mol_wt=61464
     Region          <1..80
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          122..332
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          343..519
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(352,354..358,393,395..397,400,462..464,468..469,
                     472..473,509)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(354,397)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(370,373..374,377,381,393..394,450,453,463..466,469,
                     509)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..543
                     /gene="LOC117611006"
                     /coded_by="XM_034338894.2:355..1986"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 mikatgryfc dflqsvdnih mqmrftypkm kspsmyithi dtegvilvyr strkgfthyl
       61 mgqlfqiake lyeidlnikv lgssnnipgs rsvmvkfrid fdnreyiakn nrmkvplsre
      121 lppvsctfil rlfpfgvvin kdmqilgagd kllqawggss sirdkhitev fklrrpkgis
      181 ftwgnvmylh svmfelellr lnddmssssn iastssgldr rgsqgvrsil lkgqmryied
      241 idaiiflcsp linsldelln mglylndlnp hglsrelvla gwqhcgrlem mferaeqrst
      301 elensyalld rwknksdell ysmipqtvad rlragvspls tcesfesitv lfcelcdfdy
      361 stiegamdiv ssmnavfscf dslmdefnvy kvetvgrvym aasgapdrte hhaqniadfs
      421 lqllkhvrsl klpsgldiqi rigihsgpav agvvgikvpr ycffgdtvnt asrmqttsep
      481 grihisadtk tllpqdryhv esrglvwvkg kgnmetywls dkqktniisv eviqpkrlli
      541 tdi
//
LOCUS       XP_076546021             543 aa            linear   INV 03-SEP-2025
DEFINITION  soluble guanylate cyclase 89Da isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546021
VERSION     XP_076546021.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689906.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..543
                     /product="soluble guanylate cyclase 89Da isoform X6"
                     /calculated_mol_wt=61464
     Region          <1..80
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          122..332
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          343..519
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(352,354..358,393,395..397,400,462..464,468..469,
                     472..473,509)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(354,397)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(370,373..374,377,381,393..394,450,453,463..466,469,
                     509)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..543
                     /gene="LOC117611006"
                     /coded_by="XM_076689906.1:180..1811"
                     /db_xref="GeneID:117611006"
ORIGIN      
        1 mikatgryfc dflqsvdnih mqmrftypkm kspsmyithi dtegvilvyr strkgfthyl
       61 mgqlfqiake lyeidlnikv lgssnnipgs rsvmvkfrid fdnreyiakn nrmkvplsre
      121 lppvsctfil rlfpfgvvin kdmqilgagd kllqawggss sirdkhitev fklrrpkgis
      181 ftwgnvmylh svmfelellr lnddmssssn iastssgldr rgsqgvrsil lkgqmryied
      241 idaiiflcsp linsldelln mglylndlnp hglsrelvla gwqhcgrlem mferaeqrst
      301 elensyalld rwknksdell ysmipqtvad rlragvspls tcesfesitv lfcelcdfdy
      361 stiegamdiv ssmnavfscf dslmdefnvy kvetvgrvym aasgapdrte hhaqniadfs
      421 lqllkhvrsl klpsgldiqi rigihsgpav agvvgikvpr ycffgdtvnt asrmqttsep
      481 grihisadtk tllpqdryhv esrglvwvkg kgnmetywls dkqktniisv eviqpkrlli
      541 tdi
//
LOCUS       XP_034194787             119 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611007 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194787
VERSION     XP_034194787.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338896.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194787.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..119
                     /product="uncharacterized protein LOC117611007 isoform X1"
                     /calculated_mol_wt=12940
     CDS             1..119
                     /gene="LOC117611007"
                     /coded_by="XM_034338896.2:73..432"
                     /db_xref="GeneID:117611007"
ORIGIN      
        1 mgtaqtvsnv ispqdsllal sgdlteadke nsdpslntgs qvqvpipavs htqtednltl
       61 nqppkldeev lrvlgtsvkt qeekmklhpd ltallvdwtt tdrskelket llpkypqsy
//
LOCUS       XP_076546022             100 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611007 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546022
VERSION     XP_076546022.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689907.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..100
                     /product="uncharacterized protein LOC117611007 isoform X2"
                     /calculated_mol_wt=11113
     CDS             1..100
                     /gene="LOC117611007"
                     /coded_by="XM_076689907.1:95..397"
                     /db_xref="GeneID:117611007"
ORIGIN      
        1 mrgdlteadk ensdpslntg sqvqvpipav shtqtednlt lnqppkldee vlrvlgtsvk
       61 tqeekmklhp dltallvdwt ttdrskelke tllpkypqsy
//
LOCUS       XP_034191232            1135 aa            linear   INV 03-SEP-2025
DEFINITION  disheveled-associated activator of morphogenesis-like protein
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191232
VERSION     XP_034191232.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335341.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1135
                     /product="disheveled-associated activator of
                     morphogenesis-like protein isoform X1"
                     /calculated_mol_wt=129330
     Region          90..281
                     /region_name="Drf_GBD"
                     /note="Diaphanous GTPase-binding Domain; pfam06371"
                     /db_xref="CDD:461886"
     Region          284..468
                     /region_name="Drf_FH3"
                     /note="Diaphanous FH3 Domain; pfam06367"
                     /db_xref="CDD:461885"
     Region          485..>615
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          647..1021
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     Region          <1008..1081
                     /region_name="BDP1"
                     /note="Transcription initiation factor TFIIIB, Bdp1
                     subunit [Transcription]; COG5118"
                     /db_xref="CDD:227448"
     CDS             1..1135
                     /gene="DAAM"
                     /coded_by="XM_034335341.2:181..3588"
                     /db_xref="GeneID:117609232"
ORIGIN      
        1 mspngqheni eshpeavqdd rsswffgrip scpvkspqpl kefvgrcqtm psrvkksfcg
       61 clqddeppei tycvveqtgt ltlqamtptl pmpaeeelnk mflelvdeld ltqtnrqavl
      121 alpankkwqi ycsrkgngtl engglrttdl sgdpedyinr lktiasspfs eegeeltnqm
      181 rqvealktal rtqphsfvlr fieldglnal lqvlgtmepe aansnlhtsv igclkalmnn
      241 sngrahvlah ptaintisqs lateniktki svleilgavc lvpgghrkvl eamlhfqqyh
      301 fertrfqsii ndldknfgiy kdnlslktai msfinavlny gpgqvtlefr lhlryellml
      361 giqpiieklr kyenetldrh ldffemvrne dekelarkfe kehvdtksat amfdlvrrkl
      421 shtaayphll sllqhclllp ldygsypqhw llfdrivqqi vlqsegnetg vtrnpdvapi
      481 einvkeivhl lakeeelvaa rkkaeelere nsdmstrlak keqeldlrtq ekedmeasla
      541 rvkerleket smhietkqri selqdnletl srqinnekse rkrleqlvas gslpddakat
      601 ikivedevle kveskpmppp ppppplappp ppclmpaapp pmkveiiknv pqpsnplksf
      661 nwskipeqkl qgtiwseldd tklynvmdle sidkifcayq kngvsaegsi edlrtlgknk
      721 ktmsvidsrr aqnctillsk lkmtdneitr tilsmdqqni lhidmveqll kyipsseeaa
      781 lldmhqkelq sradcflyqi skvphyeqrl rslhykkkfa asiaeltprm ravleasrqv
      841 arsrrlrkll elvlalgnyv nrgnargnac gfrlaslnrl vdtksscskg ttllhylvqi
      901 lesrfrevld ieedmphvrt aarvsmadlq kevanlkngl qdvqreiefh rgqsqvlqgd
      961 mflpamrdfq aqatcrlaea edlfqdmktr fdravrlfge dsagvqpdef fgifenflqa
     1021 laearqdven mrkkieeeer rakqeqelrk rtmerknsre gilnsislsk knevnsngqt
     1081 dnkgefddli salrtgdvfg ediakfkrsk rrpvtpsgqe srrhsahred srerh
//
LOCUS       XP_034191321            1121 aa            linear   INV 03-SEP-2025
DEFINITION  disheveled-associated activator of morphogenesis-like protein
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191321
VERSION     XP_034191321.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335430.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1121
                     /product="disheveled-associated activator of
                     morphogenesis-like protein isoform X2"
                     /calculated_mol_wt=127720
     Region          76..267
                     /region_name="Drf_GBD"
                     /note="Diaphanous GTPase-binding Domain; pfam06371"
                     /db_xref="CDD:461886"
     Region          270..454
                     /region_name="Drf_FH3"
                     /note="Diaphanous FH3 Domain; pfam06367"
                     /db_xref="CDD:461885"
     Region          471..>601
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          633..1007
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     Region          <994..1067
                     /region_name="BDP1"
                     /note="Transcription initiation factor TFIIIB, Bdp1
                     subunit [Transcription]; COG5118"
                     /db_xref="CDD:227448"
     CDS             1..1121
                     /gene="DAAM"
                     /coded_by="XM_034335430.2:601..3966"
                     /db_xref="GeneID:117609232"
ORIGIN      
        1 mdtvlekmgk lnlripscpv kspqplkefv grcqtmpsrv kksfcgclqd deppeitycv
       61 veqtgtltlq amtptlpmpa eeelnkmfle lvdeldltqt nrqavlalpa nkkwqiycsr
      121 kgngtlengg lrttdlsgdp edyinrlkti asspfseege eltnqmrqve alktalrtqp
      181 hsfvlrfiel dglnallqvl gtmepeaans nlhtsvigcl kalmnnsngr ahvlahptai
      241 ntisqslate niktkisvle ilgavclvpg ghrkvleaml hfqqyhfert rfqsiindld
      301 knfgiykdnl slktaimsfi navlnygpgq vtlefrlhlr yellmlgiqp iieklrkyen
      361 etldrhldff emvrnedeke larkfekehv dtksatamfd lvrrklshta ayphllsllq
      421 hclllpldyg sypqhwllfd rivqqivlqs egnetgvtrn pdvapieinv keivhllake
      481 eelvaarkka eelerensdm strlakkeqe ldlrtqeked measlarvke rleketsmhi
      541 etkqriselq dnletlsrqi nnekserkrl eqlvasgslp ddakatikiv edevlekves
      601 kpmppppppp plapppppcl mpaapppmkv eiiknvpqps nplksfnwsk ipeqklqgti
      661 wselddtkly nvmdlesidk ifcayqkngv saegsiedlr tlgknkktms vidsrraqnc
      721 tillsklkmt dneitrtils mdqqnilhid mveqllkyip sseeaalldm hqkelqsrad
      781 cflyqiskvp hyeqrlrslh ykkkfaasia eltprmravl easrqvarsr rlrkllelvl
      841 algnyvnrgn argnacgfrl aslnrlvdtk sscskgttll hylvqilesr frevldieed
      901 mphvrtaarv smadlqkeva nlknglqdvq reiefhrgqs qvlqgdmflp amrdfqaqat
      961 crlaeaedlf qdmktrfdra vrlfgedsag vqpdeffgif enflqalaea rqdvenmrkk
     1021 ieeeerrakq eqelrkrtme rknsregiln sislskknev nsngqtdnkg efddlisalr
     1081 tgdvfgedia kfkrskrrpv tpsgqesrrh sahredsrer h
//
LOCUS       XP_034191405            1086 aa            linear   INV 03-SEP-2025
DEFINITION  disheveled-associated activator of morphogenesis-like protein
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191405
VERSION     XP_034191405.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335514.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1086
                     /product="disheveled-associated activator of
                     morphogenesis-like protein isoform X3"
                     /calculated_mol_wt=123793
     Region          41..232
                     /region_name="Drf_GBD"
                     /note="Diaphanous GTPase-binding Domain; pfam06371"
                     /db_xref="CDD:461886"
     Region          235..419
                     /region_name="Drf_FH3"
                     /note="Diaphanous FH3 Domain; pfam06367"
                     /db_xref="CDD:461885"
     Region          436..>566
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          598..972
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     Region          <959..1032
                     /region_name="BDP1"
                     /note="Transcription initiation factor TFIIIB, Bdp1
                     subunit [Transcription]; COG5118"
                     /db_xref="CDD:227448"
     CDS             1..1086
                     /gene="DAAM"
                     /coded_by="XM_034335514.2:686..3946"
                     /db_xref="GeneID:117609232"
ORIGIN      
        1 mpsrvkksfc gclqddeppe itycvveqtg tltlqamtpt lpmpaeeeln kmflelvdel
       61 dltqtnrqav lalpankkwq iycsrkgngt lengglrttd lsgdpedyin rlktiasspf
      121 seegeeltnq mrqvealkta lrtqphsfvl rfieldglna llqvlgtmep eaansnlhts
      181 vigclkalmn nsngrahvla hptaintisq slateniktk isvleilgav clvpgghrkv
      241 leamlhfqqy hfertrfqsi indldknfgi ykdnlslkta imsfinavln ygpgqvtlef
      301 rlhlryellm lgiqpiiekl rkyenetldr hldffemvrn edekelarkf ekehvdtksa
      361 tamfdlvrrk lshtaayphl lsllqhclll pldygsypqh wllfdrivqq ivlqsegnet
      421 gvtrnpdvap ieinvkeivh llakeeelva arkkaeeler ensdmstrla kkeqeldlrt
      481 qekedmeasl arvkerleke tsmhietkqr iselqdnlet lsrqinneks erkrleqlva
      541 sgslpddaka tikivedevl ekveskpmpp pppppplapp pppclmpaap ppmkveiikn
      601 vpqpsnplks fnwskipeqk lqgtiwseld dtklynvmdl esidkifcay qkngvsaegs
      661 iedlrtlgkn kktmsvidsr raqnctills klkmtdneit rtilsmdqqn ilhidmveql
      721 lkyipsseea alldmhqkel qsradcflyq iskvphyeqr lrslhykkkf aasiaeltpr
      781 mravleasrq varsrrlrkl lelvlalgny vnrgnargna cgfrlaslnr lvdtksscsk
      841 gttllhylvq ilesrfrevl dieedmphvr taarvsmadl qkevanlkng lqdvqreief
      901 hrgqsqvlqg dmflpamrdf qaqatcrlae aedlfqdmkt rfdravrlfg edsagvqpde
      961 ffgifenflq alaearqdve nmrkkieeee rrakqeqelr krtmerknsr egilnsisls
     1021 kknevnsngq tdnkgefddl isalrtgdvf gediakfkrs krrpvtpsgq esrrhsahre
     1081 dsrerh
//
LOCUS       XP_034191494            1086 aa            linear   INV 03-SEP-2025
DEFINITION  disheveled-associated activator of morphogenesis-like protein
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191494
VERSION     XP_034191494.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335603.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1086
                     /product="disheveled-associated activator of
                     morphogenesis-like protein isoform X3"
                     /calculated_mol_wt=123793
     Region          41..232
                     /region_name="Drf_GBD"
                     /note="Diaphanous GTPase-binding Domain; pfam06371"
                     /db_xref="CDD:461886"
     Region          235..419
                     /region_name="Drf_FH3"
                     /note="Diaphanous FH3 Domain; pfam06367"
                     /db_xref="CDD:461885"
     Region          436..>566
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          598..972
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     Region          <959..1032
                     /region_name="BDP1"
                     /note="Transcription initiation factor TFIIIB, Bdp1
                     subunit [Transcription]; COG5118"
                     /db_xref="CDD:227448"
     CDS             1..1086
                     /gene="DAAM"
                     /coded_by="XM_034335603.2:996..4256"
                     /db_xref="GeneID:117609232"
ORIGIN      
        1 mpsrvkksfc gclqddeppe itycvveqtg tltlqamtpt lpmpaeeeln kmflelvdel
       61 dltqtnrqav lalpankkwq iycsrkgngt lengglrttd lsgdpedyin rlktiasspf
      121 seegeeltnq mrqvealkta lrtqphsfvl rfieldglna llqvlgtmep eaansnlhts
      181 vigclkalmn nsngrahvla hptaintisq slateniktk isvleilgav clvpgghrkv
      241 leamlhfqqy hfertrfqsi indldknfgi ykdnlslkta imsfinavln ygpgqvtlef
      301 rlhlryellm lgiqpiiekl rkyenetldr hldffemvrn edekelarkf ekehvdtksa
      361 tamfdlvrrk lshtaayphl lsllqhclll pldygsypqh wllfdrivqq ivlqsegnet
      421 gvtrnpdvap ieinvkeivh llakeeelva arkkaeeler ensdmstrla kkeqeldlrt
      481 qekedmeasl arvkerleke tsmhietkqr iselqdnlet lsrqinneks erkrleqlva
      541 sgslpddaka tikivedevl ekveskpmpp pppppplapp pppclmpaap ppmkveiikn
      601 vpqpsnplks fnwskipeqk lqgtiwseld dtklynvmdl esidkifcay qkngvsaegs
      661 iedlrtlgkn kktmsvidsr raqnctills klkmtdneit rtilsmdqqn ilhidmveql
      721 lkyipsseea alldmhqkel qsradcflyq iskvphyeqr lrslhykkkf aasiaeltpr
      781 mravleasrq varsrrlrkl lelvlalgny vnrgnargna cgfrlaslnr lvdtksscsk
      841 gttllhylvq ilesrfrevl dieedmphvr taarvsmadl qkevanlkng lqdvqreief
      901 hrgqsqvlqg dmflpamrdf qaqatcrlae aedlfqdmkt rfdravrlfg edsagvqpde
      961 ffgifenflq alaearqdve nmrkkieeee rrakqeqelr krtmerknsr egilnsisls
     1021 kknevnsngq tdnkgefddl isalrtgdvf gediakfkrs krrpvtpsgq esrrhsahre
     1081 dsrerh
//
LOCUS       XP_034191574            1086 aa            linear   INV 03-SEP-2025
DEFINITION  disheveled-associated activator of morphogenesis-like protein
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191574
VERSION     XP_034191574.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335683.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1086
                     /product="disheveled-associated activator of
                     morphogenesis-like protein isoform X3"
                     /calculated_mol_wt=123793
     Region          41..232
                     /region_name="Drf_GBD"
                     /note="Diaphanous GTPase-binding Domain; pfam06371"
                     /db_xref="CDD:461886"
     Region          235..419
                     /region_name="Drf_FH3"
                     /note="Diaphanous FH3 Domain; pfam06367"
                     /db_xref="CDD:461885"
     Region          436..>566
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          598..972
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     Region          <959..1032
                     /region_name="BDP1"
                     /note="Transcription initiation factor TFIIIB, Bdp1
                     subunit [Transcription]; COG5118"
                     /db_xref="CDD:227448"
     CDS             1..1086
                     /gene="DAAM"
                     /coded_by="XM_034335683.2:232..3492"
                     /db_xref="GeneID:117609232"
ORIGIN      
        1 mpsrvkksfc gclqddeppe itycvveqtg tltlqamtpt lpmpaeeeln kmflelvdel
       61 dltqtnrqav lalpankkwq iycsrkgngt lengglrttd lsgdpedyin rlktiasspf
      121 seegeeltnq mrqvealkta lrtqphsfvl rfieldglna llqvlgtmep eaansnlhts
      181 vigclkalmn nsngrahvla hptaintisq slateniktk isvleilgav clvpgghrkv
      241 leamlhfqqy hfertrfqsi indldknfgi ykdnlslkta imsfinavln ygpgqvtlef
      301 rlhlryellm lgiqpiiekl rkyenetldr hldffemvrn edekelarkf ekehvdtksa
      361 tamfdlvrrk lshtaayphl lsllqhclll pldygsypqh wllfdrivqq ivlqsegnet
      421 gvtrnpdvap ieinvkeivh llakeeelva arkkaeeler ensdmstrla kkeqeldlrt
      481 qekedmeasl arvkerleke tsmhietkqr iselqdnlet lsrqinneks erkrleqlva
      541 sgslpddaka tikivedevl ekveskpmpp pppppplapp pppclmpaap ppmkveiikn
      601 vpqpsnplks fnwskipeqk lqgtiwseld dtklynvmdl esidkifcay qkngvsaegs
      661 iedlrtlgkn kktmsvidsr raqnctills klkmtdneit rtilsmdqqn ilhidmveql
      721 lkyipsseea alldmhqkel qsradcflyq iskvphyeqr lrslhykkkf aasiaeltpr
      781 mravleasrq varsrrlrkl lelvlalgny vnrgnargna cgfrlaslnr lvdtksscsk
      841 gttllhylvq ilesrfrevl dieedmphvr taarvsmadl qkevanlkng lqdvqreief
      901 hrgqsqvlqg dmflpamrdf qaqatcrlae aedlfqdmkt rfdravrlfg edsagvqpde
      961 ffgifenflq alaearqdve nmrkkieeee rrakqeqelr krtmerknsr egilnsisls
     1021 kknevnsngq tdnkgefddl isalrtgdvf gediakfkrs krrpvtpsgq esrrhsahre
     1081 dsrerh
//
LOCUS       XP_034191661            1086 aa            linear   INV 03-SEP-2025
DEFINITION  disheveled-associated activator of morphogenesis-like protein
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191661
VERSION     XP_034191661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1086
                     /product="disheveled-associated activator of
                     morphogenesis-like protein isoform X3"
                     /calculated_mol_wt=123793
     Region          41..232
                     /region_name="Drf_GBD"
                     /note="Diaphanous GTPase-binding Domain; pfam06371"
                     /db_xref="CDD:461886"
     Region          235..419
                     /region_name="Drf_FH3"
                     /note="Diaphanous FH3 Domain; pfam06367"
                     /db_xref="CDD:461885"
     Region          436..>566
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          598..972
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     Region          <959..1032
                     /region_name="BDP1"
                     /note="Transcription initiation factor TFIIIB, Bdp1
                     subunit [Transcription]; COG5118"
                     /db_xref="CDD:227448"
     CDS             1..1086
                     /gene="DAAM"
                     /coded_by="XM_034335770.2:293..3553"
                     /db_xref="GeneID:117609232"
ORIGIN      
        1 mpsrvkksfc gclqddeppe itycvveqtg tltlqamtpt lpmpaeeeln kmflelvdel
       61 dltqtnrqav lalpankkwq iycsrkgngt lengglrttd lsgdpedyin rlktiasspf
      121 seegeeltnq mrqvealkta lrtqphsfvl rfieldglna llqvlgtmep eaansnlhts
      181 vigclkalmn nsngrahvla hptaintisq slateniktk isvleilgav clvpgghrkv
      241 leamlhfqqy hfertrfqsi indldknfgi ykdnlslkta imsfinavln ygpgqvtlef
      301 rlhlryellm lgiqpiiekl rkyenetldr hldffemvrn edekelarkf ekehvdtksa
      361 tamfdlvrrk lshtaayphl lsllqhclll pldygsypqh wllfdrivqq ivlqsegnet
      421 gvtrnpdvap ieinvkeivh llakeeelva arkkaeeler ensdmstrla kkeqeldlrt
      481 qekedmeasl arvkerleke tsmhietkqr iselqdnlet lsrqinneks erkrleqlva
      541 sgslpddaka tikivedevl ekveskpmpp pppppplapp pppclmpaap ppmkveiikn
      601 vpqpsnplks fnwskipeqk lqgtiwseld dtklynvmdl esidkifcay qkngvsaegs
      661 iedlrtlgkn kktmsvidsr raqnctills klkmtdneit rtilsmdqqn ilhidmveql
      721 lkyipsseea alldmhqkel qsradcflyq iskvphyeqr lrslhykkkf aasiaeltpr
      781 mravleasrq varsrrlrkl lelvlalgny vnrgnargna cgfrlaslnr lvdtksscsk
      841 gttllhylvq ilesrfrevl dieedmphvr taarvsmadl qkevanlkng lqdvqreief
      901 hrgqsqvlqg dmflpamrdf qaqatcrlae aedlfqdmkt rfdravrlfg edsagvqpde
      961 ffgifenflq alaearqdve nmrkkieeee rrakqeqelr krtmerknsr egilnsisls
     1021 kknevnsngq tdnkgefddl isalrtgdvf gediakfkrs krrpvtpsgq esrrhsahre
     1081 dsrerh
//
LOCUS       XP_076546023             870 aa            linear   INV 03-SEP-2025
DEFINITION  bestrophin family protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546023
VERSION     XP_076546023.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689908.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..870
                     /product="bestrophin family protein isoform X1"
                     /calculated_mol_wt=96973
     Region          37..331
                     /region_name="Bestrophin"
                     /note="RFP-TM, chloride channel; pfam01062"
                     /db_xref="CDD:460047"
     Region          530..>860
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..870
                     /gene="Best2"
                     /coded_by="XM_076689908.1:264..2876"
                     /db_xref="GeneID:117602598"
ORIGIN      
        1 mcrvldleps ieggegrksl gkdsanarer tkgepirwrg svykliwrel lvylfvyyli
       61 nltyryalne hqrvifekir yyfgnssesi pmsfvlgfyv slvvkrwweq ykllpwpdnl
      121 alfisaaipg ndergrlmrr nivryavlay vitlqrislr vkrrfptlqh ivdvglmmes
      181 ekkifemmnk kaamskywmp lvwatniinr arrealitsd qvvqtllvel sdirkrlgal
      241 igydtvcvpl vytqvvtlsl yayffsallg rqfiertdag ggkyeepdmy fpfftalqfc
      301 fyvgwlkvae vlinpfgedd ddielnwlid rhikagymiv demheehpel lkdqywdevv
      361 pkdlpytvas eqyrreepkg saehykvkes dalyanvmlg tqihnhvqhr kthqddmyad
      421 yesvdtplve rrknwlqrqi trmgsvrsss ttyssgggff srnrhnsvys spetgglpqt
      481 nnpnlkmsly drlvgrksvr sqrmgrqgtm tklnsvpvsl knrpriptpd vtkevvdreq
      541 rlalsatnav nigagvvgvi panghyqtdl pvvqvmlsti qeaegtpatg ksgaaalaqa
      601 vlsptltsag lvgpvtltpv tmsqltqlgl vtttsapmik psntasnvqa tltevnssee
      661 egsgssssrs gsitgqeers tplinqdrst pligeaasrl gssgssptfd sysdrspilm
      721 speklgymvt tvpivqdtkt vndprgrrsa slpgppllps redrsmslph spglqprenr
      781 aasvsnghep pnidrllvha nqdvrprtns ighdlcrpih riqdnsrkis svsctnatla
      841 gglgttptsa ttvaptqapv agskrgevyv
//
LOCUS       XP_076546024             867 aa            linear   INV 03-SEP-2025
DEFINITION  bestrophin family protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546024
VERSION     XP_076546024.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689909.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..867
                     /product="bestrophin family protein isoform X2"
                     /calculated_mol_wt=96631
     Region          37..331
                     /region_name="Bestrophin"
                     /note="RFP-TM, chloride channel; pfam01062"
                     /db_xref="CDD:460047"
     Region          502..>857
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..867
                     /gene="Best2"
                     /coded_by="XM_076689909.1:267..2870"
                     /db_xref="GeneID:117602598"
ORIGIN      
        1 mcrvldleps ieggegrksl gkdsanarer tkgepirwrg svykliwrel lvylfvyyli
       61 nltyryalne hqrvifekir yyfgnssesi pmsfvlgfyv slvvkrwweq ykllpwpdnl
      121 alfisaaipg ndergrlmrr nivryavlay vitlqrislr vkrrfptlqh ivdvglmmes
      181 ekkifemmnk kaamskywmp lvwatniinr arrealitsd qvvqtllvel sdirkrlgal
      241 igydtvcvpl vytqvvtlsl yayffsallg rqfiertdag ggkyeepdmy fpfftalqfc
      301 fyvgwlkvae vlinpfgedd ddielnwlid rhikagymiv demheehpel lkdqywdevv
      361 pkdlpytvas eqyrreepkg saehykvkes dalyanvmlg tqihnhvqhr kthqddmyad
      421 yesvdtplve rrknwlqrqi trmgsvrsss ttyssgggff srnrhnsvys spetgglpqt
      481 nnpnlkmsly drlvgrksvr sqrmgtmtkl nsvpvslknr priptpdvtk evvdreqrla
      541 lsatnavnig agvvgvipan ghyqtdlpvv qvmlstiqea egtpatgksg aaalaqavls
      601 ptltsaglvg pvtltpvtms qltqlglvtt tsapmikpsn tasnvqatlt evnsseeegs
      661 gssssrsgsi tgqeerstpl inqdrstpli geaasrlgss gssptfdsys drspilmspe
      721 klgymvttvp ivqdtktvnd prgrrsaslp gppllpsred rsmslphspg lqprenraas
      781 vsngheppni drllvhanqd vrprtnsigh dlcrpihriq dnsrkissvs ctnatlaggl
      841 gttptsattv aptqapvags krgevyv
//
LOCUS       XP_034176720             857 aa            linear   INV 03-SEP-2025
DEFINITION  bestrophin family protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034176720
VERSION     XP_034176720.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320829.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..857
                     /product="bestrophin family protein isoform X3"
                     /calculated_mol_wt=95623
     Region          13..318
                     /region_name="Bestrophin"
                     /note="RFP-TM, chloride channel; pfam01062"
                     /db_xref="CDD:460047"
     Region          517..>847
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..857
                     /gene="Best2"
                     /coded_by="XM_034320829.2:345..2918"
                     /db_xref="GeneID:117602598"
ORIGIN      
        1 mtisyasevp ngssfgcfwr ilikwrgsvy kliwrellvy lfvyylinlt yryalnehqr
       61 vifekiryyf gnssesipms fvlgfyvslv vkrwweqykl lpwpdnlalf isaaipgnde
      121 rgrlmrrniv ryavlayvit lqrislrvkr rfptlqhivd vglmmesekk ifemmnkkaa
      181 mskywmplvw atniinrarr ealitsdqvv qtllvelsdi rkrlgaligy dtvcvplvyt
      241 qvvtlslyay ffsallgrqf iertdagggk yeepdmyfpf ftalqfcfyv gwlkvaevli
      301 npfgeddddi elnwlidrhi kagymivdem heehpellkd qywdevvpkd lpytvaseqy
      361 rreepkgsae hykvkesdal yanvmlgtqi hnhvqhrkth qddmyadyes vdtplverrk
      421 nwlqrqitrm gsvrssstty ssgggffsrn rhnsvysspe tgglpqtnnp nlkmslydrl
      481 vgrksvrsqr mgrqgtmtkl nsvpvslknr priptpdvtk evvdreqrla lsatnavnig
      541 agvvgvipan ghyqtdlpvv qvmlstiqea egtpatgksg aaalaqavls ptltsaglvg
      601 pvtltpvtms qltqlglvtt tsapmikpsn tasnvqatlt evnsseeegs gssssrsgsi
      661 tgqeerstpl inqdrstpli geaasrlgss gssptfdsys drspilmspe klgymvttvp
      721 ivqdtktvnd prgrrsaslp gppllpsred rsmslphspg lqprenraas vsngheppni
      781 drllvhanqd vrprtnsigh dlcrpihriq dnsrkissvs ctnatlaggl gttptsattv
      841 aptqapvags krgevyv
//
LOCUS       XP_034176722             854 aa            linear   INV 03-SEP-2025
DEFINITION  bestrophin family protein isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034176722
VERSION     XP_034176722.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..854
                     /product="bestrophin family protein isoform X4"
                     /calculated_mol_wt=95282
     Region          13..318
                     /region_name="Bestrophin"
                     /note="RFP-TM, chloride channel; pfam01062"
                     /db_xref="CDD:460047"
     Region          489..>844
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..854
                     /gene="Best2"
                     /coded_by="XM_034320831.2:346..2910"
                     /db_xref="GeneID:117602598"
ORIGIN      
        1 mtisyasevp ngssfgcfwr ilikwrgsvy kliwrellvy lfvyylinlt yryalnehqr
       61 vifekiryyf gnssesipms fvlgfyvslv vkrwweqykl lpwpdnlalf isaaipgnde
      121 rgrlmrrniv ryavlayvit lqrislrvkr rfptlqhivd vglmmesekk ifemmnkkaa
      181 mskywmplvw atniinrarr ealitsdqvv qtllvelsdi rkrlgaligy dtvcvplvyt
      241 qvvtlslyay ffsallgrqf iertdagggk yeepdmyfpf ftalqfcfyv gwlkvaevli
      301 npfgeddddi elnwlidrhi kagymivdem heehpellkd qywdevvpkd lpytvaseqy
      361 rreepkgsae hykvkesdal yanvmlgtqi hnhvqhrkth qddmyadyes vdtplverrk
      421 nwlqrqitrm gsvrssstty ssgggffsrn rhnsvysspe tgglpqtnnp nlkmslydrl
      481 vgrksvrsqr mgtmtklnsv pvslknrpri ptpdvtkevv dreqrlalsa tnavnigagv
      541 vgvipanghy qtdlpvvqvm lstiqeaegt patgksgaaa laqavlsptl tsaglvgpvt
      601 ltpvtmsqlt qlglvtttsa pmikpsntas nvqatltevn sseeegsgss ssrsgsitgq
      661 eerstplinq drstpligea asrlgssgss ptfdsysdrs pilmspeklg ymvttvpivq
      721 dtktvndprg rrsaslpgpp llpsredrsm slphspglqp renraasvsn gheppnidrl
      781 lvhanqdvrp rtnsighdlc rpihriqdns rkissvsctn atlagglgtt ptsattvapt
      841 qapvagskrg evyv
//
LOCUS       XP_034176723             804 aa            linear   INV 03-SEP-2025
DEFINITION  bestrophin family protein isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034176723
VERSION     XP_034176723.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320832.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..804
                     /product="bestrophin family protein isoform X5"
                     /calculated_mol_wt=89008
     Region          14..265
                     /region_name="Bestrophin"
                     /note="RFP-TM, chloride channel; pfam01062"
                     /db_xref="CDD:460047"
     Region          464..>794
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..804
                     /gene="Best2"
                     /coded_by="XM_034320832.2:217..2631"
                     /db_xref="GeneID:117602598"
ORIGIN      
        1 mrststsgif ekiryyfgns sesipmsfvl gfyvslvvkr wweqykllpw pdnlalfisa
       61 aipgndergr lmrrnivrya vlayvitlqr islrvkrrfp tlqhivdvgl mmesekkife
      121 mmnkkaamsk ywmplvwatn iinrarreal itsdqvvqtl lvelsdirkr lgaligydtv
      181 cvplvytqvv tlslyayffs allgrqfier tdagggkyee pdmyfpffta lqfcfyvgwl
      241 kvaevlinpf geddddieln wlidrhikag ymivdemhee hpellkdqyw devvpkdlpy
      301 tvaseqyrre epkgsaehyk vkesdalyan vmlgtqihnh vqhrkthqdd myadyesvdt
      361 plverrknwl qrqitrmgsv rsssttyssg ggffsrnrhn svysspetgg lpqtnnpnlk
      421 mslydrlvgr ksvrsqrmgr qgtmtklnsv pvslknrpri ptpdvtkevv dreqrlalsa
      481 tnavnigagv vgvipanghy qtdlpvvqvm lstiqeaegt patgksgaaa laqavlsptl
      541 tsaglvgpvt ltpvtmsqlt qlglvtttsa pmikpsntas nvqatltevn sseeegsgss
      601 ssrsgsitgq eerstplinq drstpligea asrlgssgss ptfdsysdrs pilmspeklg
      661 ymvttvpivq dtktvndprg rrsaslpgpp llpsredrsm slphspglqp renraasvsn
      721 gheppnidrl lvhanqdvrp rtnsighdlc rpihriqdns rkissvsctn atlagglgtt
      781 ptsattvapt qapvagskrg evyv
//
LOCUS       XP_034176730             804 aa            linear   INV 03-SEP-2025
DEFINITION  bestrophin family protein isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034176730
VERSION     XP_034176730.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320839.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..804
                     /product="bestrophin family protein isoform X5"
                     /calculated_mol_wt=89008
     Region          14..265
                     /region_name="Bestrophin"
                     /note="RFP-TM, chloride channel; pfam01062"
                     /db_xref="CDD:460047"
     Region          464..>794
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..804
                     /gene="Best2"
                     /coded_by="XM_034320839.2:183..2597"
                     /db_xref="GeneID:117602598"
ORIGIN      
        1 mrststsgif ekiryyfgns sesipmsfvl gfyvslvvkr wweqykllpw pdnlalfisa
       61 aipgndergr lmrrnivrya vlayvitlqr islrvkrrfp tlqhivdvgl mmesekkife
      121 mmnkkaamsk ywmplvwatn iinrarreal itsdqvvqtl lvelsdirkr lgaligydtv
      181 cvplvytqvv tlslyayffs allgrqfier tdagggkyee pdmyfpffta lqfcfyvgwl
      241 kvaevlinpf geddddieln wlidrhikag ymivdemhee hpellkdqyw devvpkdlpy
      301 tvaseqyrre epkgsaehyk vkesdalyan vmlgtqihnh vqhrkthqdd myadyesvdt
      361 plverrknwl qrqitrmgsv rsssttyssg ggffsrnrhn svysspetgg lpqtnnpnlk
      421 mslydrlvgr ksvrsqrmgr qgtmtklnsv pvslknrpri ptpdvtkevv dreqrlalsa
      481 tnavnigagv vgvipanghy qtdlpvvqvm lstiqeaegt patgksgaaa laqavlsptl
      541 tsaglvgpvt ltpvtmsqlt qlglvtttsa pmikpsntas nvqatltevn sseeegsgss
      601 ssrsgsitgq eerstplinq drstpligea asrlgssgss ptfdsysdrs pilmspeklg
      661 ymvttvpivq dtktvndprg rrsaslpgpp llpsredrsm slphspglqp renraasvsn
      721 gheppnidrl lvhanqdvrp rtnsighdlc rpihriqdns rkissvsctn atlagglgtt
      781 ptsattvapt qapvagskrg evyv
//
LOCUS       XP_034192785             376 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 26 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192785
VERSION     XP_034192785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="vacuolar protein sorting 26 isoform X1"
                     /calculated_mol_wt=42406
     Region          6..280
                     /region_name="Vps26"
                     /note="Vacuolar protein sorting-associated protein 26;
                     pfam03643"
                     /db_xref="CDD:397622"
     CDS             1..376
                     /gene="Vps26"
                     /coded_by="XM_034336894.2:141..1271"
                     /db_xref="GeneID:117610005"
ORIGIN      
        1 msffgfgqsa dieitldgae trktadikse dgkkerhlly ydgetvsgki nislrkagkl
       61 ehqgvkvefi gqielyydrg nhheftnlvk elarpgelth ntvytfefan vekpfesytg
      121 snvrlryflk vtivrrlsdi vkelelvvht lssypdmnnp ikmevgiedc lhiefeynks
      181 kyhlkdvivg kiyfllvrik ikhmeiaiik rettgsgpht ftendtiaky eimdgapvrg
      241 esipirvfla gydlaptmrd inkkfsvryy lnlvlmdeed rryfkqqeit lwrkgeksrk
      301 sqtasphmps hlvsaetgfp qgaaqngpps appsiqsgql pagnitnaed apapiegmtr
      361 eegdgegeke vspesn
//
LOCUS       XP_076546025             374 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 26 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546025
VERSION     XP_076546025.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689910.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..374
                     /product="vacuolar protein sorting 26 isoform X2"
                     /calculated_mol_wt=42262
     Region          6..278
                     /region_name="Vps26"
                     /note="Vacuolar protein sorting-associated protein 26;
                     pfam03643"
                     /db_xref="CDD:397622"
     CDS             1..374
                     /gene="Vps26"
                     /coded_by="XM_076689910.1:141..1265"
                     /db_xref="GeneID:117610005"
ORIGIN      
        1 msffgfgqsa dieitldgae trktadikse dgkkerhlly ydgetvsgki nislrkagkl
       61 ehqgvkvefi gqielyydrg nhheftnlvk elarpgelth ntvytfefan vekpfesytg
      121 snvrlryflk vtivrrlsdi vkelelvvht lssypdmnnp ikmevgiedc lhiefeynks
      181 kyhlkdvivg kiyfllvrik ikhmeiaiik rettgphtft endtiakyei mdgapvrges
      241 ipirvflagy dlaptmrdin kkfsvryyln lvlmdeedrr yfkqqeitlw rkgeksrksq
      301 tasphmpshl vsaetgfpqg aaqngppsap psiqsgqlpa gnitnaedap apiegmtree
      361 gdgegekevs pesn
//
LOCUS       XP_034192914             287 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC3 [Osmia lignaria lignaria].
ACCESSION   XP_034192914
VERSION     XP_034192914.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337023.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192914.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="palmitoyltransferase ZDHHC3"
                     /calculated_mol_wt=32821
     Region          15..>178
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; cl19890"
                     /db_xref="CDD:418707"
     Region          115..242
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..287
                     /gene="LOC117610059"
                     /coded_by="XM_034337023.2:229..1092"
                     /db_xref="GeneID:117610059"
ORIGIN      
        1 mdydymsfhk ekdvhnkcyg grlwcikdic giicavltwl liiyaefvvm aviliptint
       61 lysslntaif qsltflafas hlrtmltdpg avpkgnatke mieqmgfrdg qvifkcpkcc
      121 sikpdrahhc svcqrcirkm dhhcpwvnnc vgennqkyfv lftfyiaams lqslflciqq
      181 fttcvrqewk ecstfsppat vvlllflafe allfaiftav mlgtqlqaiw ndetgieqlk
      241 keearwvrns rwksiqavfg rfslawfspf tsppkgktkl dsylysv
//
LOCUS       XP_076546026             287 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC3 [Osmia lignaria lignaria].
ACCESSION   XP_076546026
VERSION     XP_076546026.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689911.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="palmitoyltransferase ZDHHC3"
                     /calculated_mol_wt=32821
     Region          15..>178
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; cl19890"
                     /db_xref="CDD:418707"
     Region          115..242
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..287
                     /gene="LOC117610059"
                     /coded_by="XM_076689911.1:104..967"
                     /db_xref="GeneID:117610059"
ORIGIN      
        1 mdydymsfhk ekdvhnkcyg grlwcikdic giicavltwl liiyaefvvm aviliptint
       61 lysslntaif qsltflafas hlrtmltdpg avpkgnatke mieqmgfrdg qvifkcpkcc
      121 sikpdrahhc svcqrcirkm dhhcpwvnnc vgennqkyfv lftfyiaams lqslflciqq
      181 fttcvrqewk ecstfsppat vvlllflafe allfaiftav mlgtqlqaiw ndetgieqlk
      241 keearwvrns rwksiqavfg rfslawfspf tsppkgktkl dsylysv
//
LOCUS       XP_034193049              97 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610115 [Osmia lignaria lignaria].
ACCESSION   XP_034193049
VERSION     XP_034193049.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337158.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193049.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..97
                     /product="uncharacterized protein LOC117610115"
                     /calculated_mol_wt=10874
     CDS             1..97
                     /gene="LOC117610115"
                     /coded_by="XM_034337158.2:222..515"
                     /db_xref="GeneID:117610115"
ORIGIN      
        1 malnvftlfm nflvlisfaa ntlaydpndk slkdillpeg qfeafylkgt qdeeqnavrp
       61 ahlhgsfhqy knpalvgapn saaygfrfdg krrfnyn
//
LOCUS       XP_034196124             125 aa            linear   INV 03-SEP-2025
DEFINITION  ejaculatory bulb-specific protein 3-like [Osmia lignaria lignaria].
ACCESSION   XP_034196124
VERSION     XP_034196124.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340233.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="ejaculatory bulb-specific protein 3-like"
                     /calculated_mol_wt=14117
     Region          20..111
                     /region_name="OS-D"
                     /note="Insect pheromone-binding family, A10/OS-D;
                     pfam03392"
                     /db_xref="CDD:460908"
     CDS             1..125
                     /gene="LOC117611844"
                     /coded_by="XM_034340233.2:150..527"
                     /db_xref="GeneID:117611844"
ORIGIN      
        1 mkvavvflav lvcalaneky ttkydnidld qilksdrlln nyvnclldlg sctpdgkelk
       61 kslpdaland ctkcsekqkn gsekviryli nerpqvwdrl skkydptgqy ktkfqneatk
      121 rgikl
//
LOCUS       XP_034195442             485 aa            linear   INV 03-SEP-2025
DEFINITION  b(0,+)-type amino acid transporter 1 [Osmia lignaria lignaria].
ACCESSION   XP_034195442
VERSION     XP_034195442.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339551.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="b(0,+)-type amino acid transporter 1"
                     /calculated_mol_wt=53165
     Region          1..483
                     /region_name="2A0308"
                     /note="L-type amino acid transporter; TIGR00911"
                     /db_xref="CDD:273332"
     CDS             1..485
                     /gene="LOC117611574"
                     /coded_by="XM_034339551.2:530..1987"
                     /db_xref="GeneID:117611574"
ORIGIN      
        1 mrqheekeng rialkrelgl fsavsiivav migsgifvsp tsaleksgsv gfclivwitc
       61 gilsllgala faelstvvpr sgaeyayfie afsplhryag qipafvcswv yvvllrpaev
      121 avvmltfaey svqpfsnyle dlpeesmsrl kkfiavlalg lityinltsv klyvrvqnif
      181 tickivacvf vigggiwwls tghtqllekp frgtttslgd valafysglw aydgwtsaai
      241 vteeiqrpev nilrsiliav plitvlyvsm nlmymaalti pemvgarava vlwadkvlps
      301 wlsfaiplgv vlstfgcgls iqfgvsrlcy vagreghvpr vfsfvhiekm tpaaavavqg
      361 lltlvclllg niialiefas fltwvfygfa mlsliimrrt kpdaprpyav pivvpwlvlg
      421 iaiflavipi ihdpspkylf alifillgvg iyhvyvynka rstfavrlty liqalclvva
      481 pdked
//
LOCUS       XP_034195455             451 aa            linear   INV 03-SEP-2025
DEFINITION  queuine tRNA-ribosyltransferase accessory subunit 2 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034195455
VERSION     XP_034195455.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339564.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..451
                     /product="queuine tRNA-ribosyltransferase accessory
                     subunit 2 isoform X1"
                     /calculated_mol_wt=51411
     Region          71..436
                     /region_name="TGT"
                     /note="Queuine tRNA-ribosyltransferase; pfam01702"
                     /db_xref="CDD:460299"
     CDS             1..451
                     /gene="LOC117611578"
                     /coded_by="XM_034339564.2:704..2059"
                     /db_xref="GeneID:117611578"
ORIGIN      
        1 mseltakfml fflfnlcyif frnyafqilk citlqlqfsg kkinnnecmi lnisikfnmk
       61 fntnslkscv arigtltefq ripnasfetp laliytkggs vphltkdvfq mvtkdpqlls
      121 vsltstvsml esikdcnvnf sefvgmkeyl nfltthdpcy atpsgyhqpd siplwtkngk
      181 hmltpskymd iieafkpdly vafhdgdtni nsskrrvska vhrsmvffeq cmlrhstsiv
      241 lkssemlgai eggydieart vsanflkdkp vlgyiidglh nngpdvtniq tdqikkvvsh
      301 vinllppekl kvsvggwnpl tvldlvelgv dvfdtsypyi atqnaealtf lcdhdvcnnm
      361 ehvmsfkedr ykedfspics hcecvacknh traylhhlcn tremlsmvll mihnvhqyle
      421 ffkiiresir ngtldqcrkk inlkfkqtnn s
//
LOCUS       XP_076546027             403 aa            linear   INV 03-SEP-2025
DEFINITION  queuine tRNA-ribosyltransferase accessory subunit 2 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_076546027
VERSION     XP_076546027.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689912.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="queuine tRNA-ribosyltransferase accessory
                     subunit 2 isoform X2"
                     /calculated_mol_wt=45633
     Region          23..388
                     /region_name="TGT"
                     /note="Queuine tRNA-ribosyltransferase; pfam01702"
                     /db_xref="CDD:460299"
     CDS             1..403
                     /gene="LOC117611578"
                     /coded_by="XM_076689912.1:130..1341"
                     /db_xref="GeneID:117611578"
ORIGIN      
        1 milnisikfn mkfntnslks cvarigtlte fqripnasfe tplaliytkg gsvphltkdv
       61 fqmvtkdpql lsvsltstvs mlesikdcnv nfsefvgmke ylnfltthdp cyatpsgyhq
      121 pdsiplwtkn gkhmltpsky mdiieafkpd lyvafhdgdt ninsskrrvs kavhrsmvff
      181 eqcmlrhsts ivlkssemlg aieggydiea rtvsanflkd kpvlgyiidg lhnngpdvtn
      241 iqtdqikkvv shvinllppe klkvsvggwn pltvldlvel gvdvfdtsyp yiatqnaeal
      301 tflcdhdvcn nmehvmsfke drykedfspi cshcecvack nhtraylhhl cntremlsmv
      361 llmihnvhqy leffkiires irngtldqcr kkinlkfkqt nns
//
LOCUS       XP_034195462             393 aa            linear   INV 03-SEP-2025
DEFINITION  queuine tRNA-ribosyltransferase accessory subunit 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034195462
VERSION     XP_034195462.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339571.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="queuine tRNA-ribosyltransferase accessory
                     subunit 2 isoform X3"
                     /calculated_mol_wt=44458
     Region          13..378
                     /region_name="TGT"
                     /note="Queuine tRNA-ribosyltransferase; pfam01702"
                     /db_xref="CDD:460299"
     CDS             1..393
                     /gene="LOC117611578"
                     /coded_by="XM_034339571.2:238..1419"
                     /db_xref="GeneID:117611578"
ORIGIN      
        1 mkfntnslks cvarigtlte fqripnasfe tplaliytkg gsvphltkdv fqmvtkdpql
       61 lsvsltstvs mlesikdcnv nfsefvgmke ylnfltthdp cyatpsgyhq pdsiplwtkn
      121 gkhmltpsky mdiieafkpd lyvafhdgdt ninsskrrvs kavhrsmvff eqcmlrhsts
      181 ivlkssemlg aieggydiea rtvsanflkd kpvlgyiidg lhnngpdvtn iqtdqikkvv
      241 shvinllppe klkvsvggwn pltvldlvel gvdvfdtsyp yiatqnaeal tflcdhdvcn
      301 nmehvmsfke drykedfspi cshcecvack nhtraylhhl cntremlsmv llmihnvhqy
      361 leffkiires irngtldqcr kkinlkfkqt nns
//
LOCUS       XP_034195475             388 aa            linear   INV 03-SEP-2025
DEFINITION  ZZ-type zinc finger-containing protein 3 [Osmia lignaria lignaria].
ACCESSION   XP_034195475
VERSION     XP_034195475.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339584.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..388
                     /product="ZZ-type zinc finger-containing protein 3"
                     /calculated_mol_wt=44778
     Region          150..198
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(150,185..186,188..189,191..193,195..197)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          304..355
                     /region_name="ZZ_ZZZ3"
                     /note="Zinc finger, ZZ type. Zinc finger present in ZZZ3
                     (ZZ finger containing 3) and related proteins. The ZZ
                     motif coordinates two zinc ions and most likely
                     participates in ligand binding or molecular scaffolding;
                     cd02341"
                     /db_xref="CDD:239081"
     Site            order(306,309,321,324,331,334,346,350)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239081"
     Site            order(306,309,331,334)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239081"
     Site            order(307,318,320,329)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239081"
     Site            order(319,335,352,355)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239081"
     Site            order(321,324,346,350)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239081"
     CDS             1..388
                     /gene="LOC117611590"
                     /coded_by="XM_034339584.2:1..1167"
                     /db_xref="GeneID:117611590"
ORIGIN      
        1 medeekrcqd eepsefyfes dhlalkgnkd ytallktivi leaqrtqaie dldkllsirs
       61 kalkdpisfv aqiqngelpe lpgpqkiaei pyidwsqynv aapdmrmrpq trhghvlphv
      121 ptkteqengk ilvrgrafde skpetfnqlw tveeqrrlee llieyppeev emrrwtkian
      181 algnrtpkqv ssrvqkyfik llraglpipg rgpkmkldvk rgighrhqrn nyslfrrstf
      241 fphqdmsftm adeskeqpva eeseddagns tiddnpelrh iellrqvkae keensssvfk
      301 hvgykcsicg eepltgtrwh csecqngldl cgdcavlqle dekplhdilh rlipikppqy
      361 trsydldyfp qsfsnssyny ldpnflpe
//
LOCUS       XP_034195489             255 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin 16 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195489
VERSION     XP_034195489.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339598.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="sorting nexin 16 isoform X1"
                     /calculated_mol_wt=28604
     Region          77..187
                     /region_name="PX_SNX16"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexin 16; cd07276"
                     /db_xref="CDD:132809"
     Site            order(116..118,142..143,157)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132809"
     CDS             1..255
                     /gene="Snx16"
                     /coded_by="XM_034339598.2:208..975"
                     /db_xref="GeneID:117611596"
ORIGIN      
        1 mefvggkmst sesgvgcise vtlaiskarg sqsgnvrild spdsdgsghs nsftvqrfny
       61 pdndnanpgi lqppltsddl ripivgyeim eerarftvyk lrvelkngdc wfvfrrytdf
      121 vrllsqlrrq kipisqlslp rkkwlgdnfa psfleerirg lqafvnails splligtacv
      181 reffcldepp alsdtaeesr aifealedti yhlrqqlrer daalasetsl cnelrkklhq
      241 ilserqtcpk cganq
//
LOCUS       XP_034195497             248 aa            linear   INV 03-SEP-2025
DEFINITION  sorting nexin 16 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195497
VERSION     XP_034195497.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339606.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="sorting nexin 16 isoform X2"
                     /calculated_mol_wt=27855
     Region          70..180
                     /region_name="PX_SNX16"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Sorting Nexin 16; cd07276"
                     /db_xref="CDD:132809"
     Site            order(109..111,135..136,150)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132809"
     CDS             1..248
                     /gene="Snx16"
                     /coded_by="XM_034339606.2:247..993"
                     /db_xref="GeneID:117611596"
ORIGIN      
        1 mstsesgvgc isevtlaisk argsqsgnvr ildspdsdgs ghsnsftvqr fnypdndnan
       61 pgilqpplts ddlripivgy eimeerarft vyklrvelkn gdcwfvfrry tdfvrllsql
      121 rrqkipisql slprkkwlgd nfapsfleer irglqafvna ilssplligt acvreffcld
      181 eppalsdtae esraifeale dtiyhlrqql rerdaalase tslcnelrkk lhqilserqt
      241 cpkcganq
//
LOCUS       XP_034194187             684 aa            linear   INV 03-SEP-2025
DEFINITION  acetyl coenzyme A synthase [Osmia lignaria lignaria].
ACCESSION   XP_034194187
VERSION     XP_034194187.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338296.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194187.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="acetyl coenzyme A synthase"
                     /calculated_mol_wt=76796
     Region          22..670
                     /region_name="ACS"
                     /note="Acetyl-CoA synthetase (also known as acetate-CoA
                     ligase and acetyl-activating enzyme); cd05966"
                     /db_xref="CDD:341270"
     Site            order(168..170,196,199,201,342,347..348,370..372,396..397,
                     400,423..426,448..453,537,549,552,560..563,621,626..627)
                     /site_type="active"
                     /db_xref="CDD:341270"
     Site            order(168..170,196,199,201,342,348,370..372,396..397,400,
                     560..562,621,626)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341270"
     Site            order(298,301..306,308..309)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341270"
     Site            order(347..348,423..426,448..453,537,549,552,563)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341270"
     Site            order(347..348,423..424,451)
                     /site_type="other"
                     /note="acetate binding site [chemical binding]"
                     /db_xref="CDD:341270"
     CDS             1..684
                     /gene="AcCoAS"
                     /coded_by="XM_034338296.2:318..2372"
                     /db_xref="GeneID:117610643"
ORIGIN      
        1 madeiyyphp eiaknaycns meqyrkmhek svknpaefwg eiakqfywet pasedkfysy
       61 nfdlskgdvf ikwmegastn isfnlldknv ksgngdkiaf ywegndpddy srltyrklle
      121 evcrfanvlk skgvtkgdrv aiympmilel piamlactri gavhsvvfag yssdslaeri
      181 ldskakvlit tdgvwrgekl lllkaicdea lnkvkkhghe veycvvvahl rrltspqgvk
      241 adatgktngv ngnsdgngvd tpripwdddr dvwwhdemee aedrcypvwm aaedpvfily
      301 tsgstgkpkg vlhttagyli yaattfkyvf dyhpgdvywc tadvgwitgh tyvvygplan
      361 gatsvlfegt pfypsndryw vvvdkykvsq fytaptairs lmkfgdelvk khdlstlkvl
      421 gsvgepinse awlwfynlvg hgkcsisdtf wqtetgghvi tplpgatpmk pgsatfpffg
      481 vlpelldedg rliegegegf lvfrqpwpgm mrtlygnhqr fqntyfdrfh gfyctgdgar
      541 rdadgylwit griddmlnvs ghlmstaeve svltehslva eaavvskphp vkgqclycfv
      601 tpnegasfdk klqdelkkrv rerigpfaqp divqhapglp ktrsgkimrr ilrkiaagdr
      661 nvgdvstlad esivdllfql rpqv
//
LOCUS       XP_034194295             438 aa            linear   INV 03-SEP-2025
DEFINITION  enolase [Osmia lignaria lignaria].
ACCESSION   XP_034194295
VERSION     XP_034194295.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338404.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..438
                     /product="enolase"
                     /calculated_mol_wt=48284
     Region          5..420
                     /region_name="enolase"
                     /note="Enolase: Enolases are homodimeric enzymes that
                     catalyse the reversible dehydration of
                     2-phospho-D-glycerate to phosphoenolpyruvate as part of
                     the glycolytic and gluconeogenesis pathways. The reaction
                     is facilitated by the presence of metal ions; cd03313"
                     /db_xref="CDD:239429"
     Site            order(7,9..17,21,32,159..161,182..183,186..187,190..191,
                     207..208,215,218,379..381,404..406,408..409,412,415..416,
                     419)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239429"
     Site            order(40,247,297,322)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:239429"
     Site            order(158,211,347,375..377,398)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:239429"
     CDS             1..438
                     /gene="LOC117610697"
                     /coded_by="XM_034338404.2:1..1317"
                     /db_xref="GeneID:117610697"
ORIGIN      
        1 mpiqkvkarq ifdsrgdptl evdiitdvgl lracvpavvs psanqaqelr dgneamyhgk
       61 svfravdvvn niiapqllks rleacqqtei dsllnrldgt enksklgana ilgvsiacck
      121 agaakkglpv yryiaemaen selylpvpcf nmisggrhan nvipcqefli lpigaesfad
      181 amkmgtevyr vlerkiataq eiqlplpvsd dgaftplele edkealllld esireagydg
      241 rikialdmaa safykeggyd lqfkteesdp ddymeaealr dqyleyisey pnivsiedpf
      301 dqddwegwls madqeevqiv sddltamnid rieeaierss anaivlrmlq igtvtetinc
      361 arlarisnwg yvvsacqget ednfvadlav glsagqfksg apcrseriak ynqllrieee
      421 lgkdakyagq nfrnplak
//
LOCUS       XP_034183061            3612 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605632 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183061
VERSION     XP_034183061.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327170.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183061.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3612
                     /product="uncharacterized protein LOC117605632 isoform X1"
                     /calculated_mol_wt=416810
     Region          <131..>774
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          554..1456
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1310..2067
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1901..2730
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2788..>3455
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3612
                     /gene="LOC117605632"
                     /coded_by="XM_034327170.2:1..10839"
                     /db_xref="GeneID:117605632"
ORIGIN      
        1 mkwfllliii qfimwstsee ppgtmegdss esmvhakekc eqnknqlesl keimlknkqs
       61 lkkkeeevqe yarrlskiks raklsrrskg enlpckdvsh tseettsvdi seetiddisq
      121 aktakakstl lqkklaenrk vfekrnkeit etkraveekv eairqqleek ditamgfqkd
      181 qlpvapikpv missdimspk qiesieeken qiavlnkkil eleatvvdlq enlkekdsvi
      241 esktravtlm sadlskkgkt tldtledtkd emrtmqehfi lletslknkn eslleqlqgr
      301 dnkiaeleds ikgfeqqlsq sadadvsrst ldtlletkea mksmqenfvl iesslkaknd
      361 hlleqledye lklaeaneri fklesgigii kdptvddlqy kleklehsnk qlqdekyelq
      421 kniaelqdki lnlsvngnng almekdnriv elenlieelk qsnklleees kaelqqklad
      481 ltlkneeysn kiadleglvh eleeqksdia arlsdesslk gdekvakltk eleelnksmi
      541 kikaqhrski knlqkqlenv kkisdtnael vrlgnqvall eeekgnlqls lvdfdelkas
      601 agdwqervid leskvsaqtk eiemqieaia tlenqkldlm qelhiakqev ssleaenaes
      661 enlrvtaemk ivdleeqlea mnklqndnkl esssednhae llkqvealtq enaelysris
      721 kleekgtset gstesfeaiq eldksdllkk iedlmhknne ltlkvskyqe kenshtgste
      781 wletmnerdk nellkkveql tqentdlamk lsrmeekgss dtgstesfer ipehnesmtk
      841 ielltqenne lvikftklke qlentmeida klraeievlq qadkdkssel erltaelrdl
      901 aeekdrlqkq ieslivekne laalhtklee drsvseqetv dfaerskrsv dveaeideek
      961 dllekevkgl erlrestqdl qidelngrie ellkenervl sklseletyn dklkenltet
     1021 trekeelqsk indmsrdesa kemlefekkd vvpekeempq sdessqqtes mivaslerei
     1081 eeckkliaeq tglieemklk lankeeelee kgkqlteyea sgkkaeglen elkeifdtve
     1141 ewkhkciemq ekmekleagk asieegfkml qnenkillee nkekdaetdr lkqqlqdtvt
     1201 tfeskvqeql aaisekenei gnlkqiiedk dqelhakyte lqnkmiaids lqdecnnckm
     1261 liqernnvla smsdevanln namkskedei ytlrkhidel sekldeskpl kdyselleql
     1321 knkdmildel hcrinattke nsnlseklks lsqhnnniqs qlaekqrefv dmvaakehle
     1381 tliaetkkek deaegriwel qsvidnnmkf vddlqgelrg sykhieqlkl khtedvelqn
     1441 qrlenlieel nsktqecetl rdeleekerl vgrniteevk valearivdl aeklkeseek
     1501 iqsqlekmkk laanlkkktt vcqelegrvt eleekwttek dekeaknkqi qdveiamrek
     1561 dnritdleek ltrarnenad afknieelts dlsdskekit llteqieeme qeivklradl
     1621 esrtaelgie reskesiisd ydsykqsvir eneqkqveld evkekarels vrmqvmeaey
     1681 veqlasiknl taenglllsk qtqineklen vekeseerrl lieqmrrvtt eavtestqtt
     1741 eeenteeegk tegaeqcdrc kqcqtlvral elklqereae ienldnelan signfvrmnq
     1801 nsmrnrsled pyndllfqyn slissheevk akldeasken kqliekieql qsmnvtleet
     1861 vttteqsieg hredslnsdl akereereae llnlqreiqt lrervneleq qlnsqtisle
     1921 svedskkdlr qeieslksdr etndkliedl rnelegyrnq sgesvekpia kreatfdqqe
     1981 naprlfdask ifgsfasdsd lltdneakrl qalleekear csnltqemnh lqklmmeers
     2041 qmsqnysqcv eeleisrkel tttlsnlevl ertlsekdkq idalnlelqn lnlrmmeqqn
     2101 dtkesqleel rtslnstkea ldaiivereq rnallesykl qvnnleaelk nstdseliqe
     2161 lesrvscttk erdllqlqvn dlsrsmeelk ysmgveleki grerdeakem vanltraled
     2221 aykqsmtsti tdtstkestp leevprdqle pgspatkavv eeakqgtast plssevpfvd
     2281 dgtgwdaekl vdeeswgwda eeaqlvgehv taslipsmem qlrarvddle drikdmeker
     2341 drmleeskav qlrnakmikk lkeykvqves lqqqlktqks ssdfyeldsa ieeelkcqis
     2401 klekslsevr eelkntvaek ealtkrldvi vsanerymem kerqdmdmev lrirnkelsd
     2461 kiealdkklq sgvtaaedse vpraesvvsg eedkpvprer vrkrsvdped yeslckkyre
     2521 eiddlkeeme alasendllq hfleeqkvkm sslekkrtae enesiqivdd lnkriselqa
     2581 mlsksreeye llrkqyeqsl mdandqvtam rqntdflkee arekigklem evadlrkqle
     2641 ifessnaelq khlqevfqek ssleerltdl massekqlss mnasmaevtd llniriqeva
     2701 dlkqelqrqy veheeaktkl qetveglnqe lgekrqqles lkqslseken efvqqqsvet
     2761 vsalvsqatq elvqkhaiei eekekeiqsl lgklsaletv vneyssekqs ilarldeqqq
     2821 elqllrqdlt eknsslvafq tnltsilsek eqelfdrner idrltadnqs lletigelqr
     2881 rlyefettsi dtsgltsriq sletslleke sdlarcakef neyrailenn rieieqlrad
     2941 krdidelkse ltakaeqmnr lnveleatrk algetrqiln ektcllekcn raleekdses
     3001 savpmvesnq psasnivdgl plfrmtednh gfqqtidgmq aelekkqeei ehlkyvlnen
     3061 typgiiremq dtinclydek aelkssldal nekfeekake ieslteslka kslevvskeq
     3121 tgllsreras mqdqerivgl qnelhakeqe inemkyvlae kdsqlslqas mepqsddfel
     3181 remverltge vygkeqevqq lksiiaelqk evsrlqefer lseetkdaie klgdekeqar
     3241 leaeeylkrt leqketeide ikqrlsvenq rmldelqlrd rdvenlkkql eelstmeqtm
     3301 kdklqgkeed larvsndlae kerrlaelsi tkdaelhnln vqihekeari eelmvlsdee
     3361 ekqlkelrnv leareteins lkklledkvk efeliqnvlk kdvsvvgast sqqgetsdee
     3421 nkafasqeld lalymlhqrd vrceelthel mqlleerdtl qlrlsnairv neelrrmggg
     3481 sdvaprdeas tsvseepmve hpspsksegp veiakeaida pigedkeala lklsqlhtvs
     3541 htkdvrlrde relrhtqqms llahkdvlst lppeaaarlv nanytlsrdv qsqssvllnw
     3601 lwgkstpkvv hm
//
LOCUS       XP_034183069            3599 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605632 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183069
VERSION     XP_034183069.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327178.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183069.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3599
                     /product="uncharacterized protein LOC117605632 isoform X2"
                     /calculated_mol_wt=415150
     Region          <118..>761
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          541..1443
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1297..2054
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1888..2717
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2775..>3442
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3599
                     /gene="LOC117605632"
                     /coded_by="XM_034327178.2:262..11061"
                     /db_xref="GeneID:117605632"
ORIGIN      
        1 mwstseeppg tmegdssesm vhakekceqn knqleslkei mlknkqslkk keeevqeyar
       61 rlskiksrak lsrrskgenl pckdvshtse ettsvdisee tiddisqakt akakstllqk
      121 klaenrkvfe krnkeitetk raveekveai rqqleekdit amgfqkdqlp vapikpvmis
      181 sdimspkqie sieekenqia vlnkkilele atvvdlqenl kekdsviesk travtlmsad
      241 lskkgkttld tledtkdemr tmqehfille tslknknesl leqlqgrdnk iaeledsikg
      301 feqqlsqsad advsrstldt lletkeamks mqenfvlies slkakndhll eqledyelkl
      361 aeanerifkl esgigiikdp tvddlqykle klehsnkqlq dekyelqkni aelqdkilnl
      421 svngnngalm ekdnrivele nlieelkqsn klleeeskae lqqkladltl kneeysnkia
      481 dleglvhele eqksdiaarl sdesslkgde kvakltkele elnksmikik aqhrskiknl
      541 qkqlenvkki sdtnaelvrl gnqvalleee kgnlqlslvd fdelkasagd wqervidles
      601 kvsaqtkeie mqieaiatle nqkldlmqel hiakqevssl eaenaesenl rvtaemkivd
      661 leeqleamnk lqndnkless sednhaellk qvealtqena elysriskle ekgtsetgst
      721 esfeaiqeld ksdllkkied lmhknneltl kvskyqeken shtgstewle tmnerdknel
      781 lkkveqltqe ntdlamklsr meekgssdtg stesferipe hnesmtkiel ltqennelvi
      841 kftklkeqle ntmeidaklr aeievlqqad kdksselerl taelrdlaee kdrlqkqies
      901 liveknelaa lhtkleedrs vseqetvdfa erskrsvdve aeideekdll ekevkglerl
      961 restqdlqid elngrieell kenervlskl seletyndkl kenltettre keelqskind
     1021 msrdesakem lefekkdvvp ekeempqsde ssqqtesmiv aslereieec kkliaeqtgl
     1081 ieemklklan keeeleekgk qlteyeasgk kaeglenelk eifdtveewk hkciemqekm
     1141 ekleagkasi eegfkmlqne nkilleenke kdaetdrlkq qlqdtvttfe skvqeqlaai
     1201 sekeneignl kqiiedkdqe lhakytelqn kmiaidslqd ecnnckmliq ernnvlasms
     1261 devanlnnam kskedeiytl rkhidelsek ldeskplkdy selleqlknk dmildelhcr
     1321 inattkensn lseklkslsq hnnniqsqla ekqrefvdmv aakehletli aetkkekdea
     1381 egriwelqsv idnnmkfvdd lqgelrgsyk hieqlklkht edvelqnqrl enlieelnsk
     1441 tqecetlrde leekerlvgr niteevkval earivdlaek lkeseekiqs qlekmkklaa
     1501 nlkkkttvcq elegrvtele ekwttekdek eaknkqiqdv eiamrekdnr itdleekltr
     1561 arnenadafk nieeltsdls dskekitllt eqieemeqei vklradlesr taelgieres
     1621 kesiisdyds ykqsvirene qkqveldevk ekarelsvrm qvmeaeyveq lasiknltae
     1681 nglllskqtq ineklenvek eseerrllie qmrrvtteav testqtteee nteeegkteg
     1741 aeqcdrckqc qtlvralelk lqereaeien ldnelansig nfvrmnqnsm rnrsledpyn
     1801 dllfqynsli ssheevkakl deaskenkql iekieqlqsm nvtleetvtt teqsieghre
     1861 dslnsdlake reereaelln lqreiqtlre rvneleqqln sqtislesve dskkdlrqei
     1921 eslksdretn dkliedlrne legyrnqsge svekpiakre atfdqqenap rlfdaskifg
     1981 sfasdsdllt dneakrlqal leekearcsn ltqemnhlqk lmmeersqms qnysqcveel
     2041 eisrkelttt lsnlevlert lsekdkqida lnlelqnlnl rmmeqqndtk esqleelrts
     2101 lnstkealda iivereqrna llesyklqvn nleaelknst dseliqeles rvscttkerd
     2161 llqlqvndls rsmeelkysm gvelekigre rdeakemvan ltraledayk qsmtstitdt
     2221 stkestplee vprdqlepgs patkavveea kqgtastpls sevpfvddgt gwdaeklvde
     2281 eswgwdaeea qlvgehvtas lipsmemqlr arvddledri kdmekerdrm leeskavqlr
     2341 nakmikklke ykvqveslqq qlktqksssd fyeldsaiee elkcqiskle kslsevreel
     2401 kntvaekeal tkrldvivsa nerymemker qdmdmevlri rnkelsdkie aldkklqsgv
     2461 taaedsevpr aesvvsgeed kpvprervrk rsvdpedyes lckkyreeid dlkeemeala
     2521 sendllqhfl eeqkvkmssl ekkrtaeene siqivddlnk riselqamls ksreeyellr
     2581 kqyeqslmda ndqvtamrqn tdflkeeare kigklemeva dlrkqleife ssnaelqkhl
     2641 qevfqekssl eerltdlmas sekqlssmna smaevtdlln iriqevadlk qelqrqyveh
     2701 eeaktklqet veglnqelge krqqleslkq slsekenefv qqqsvetvsa lvsqatqelv
     2761 qkhaieieek ekeiqsllgk lsaletvvne yssekqsila rldeqqqelq llrqdltekn
     2821 sslvafqtnl tsilsekeqe lfdrneridr ltadnqslle tigelqrrly efettsidts
     2881 gltsriqsle tsllekesdl arcakefney railennrie ieqlradkrd idelkselta
     2941 kaeqmnrlnv eleatrkalg etrqilnekt cllekcnral eekdsessav pmvesnqpsa
     3001 snivdglplf rmtednhgfq qtidgmqael ekkqeeiehl kyvlnentyp giiremqdti
     3061 nclydekael kssldalnek feekakeies lteslkaksl evvskeqtgl lsrerasmqd
     3121 qerivglqne lhakeqeine mkyvlaekds qlslqasmep qsddfelrem verltgevyg
     3181 keqevqqlks iiaelqkevs rlqeferlse etkdaieklg dekeqarlea eeylkrtleq
     3241 keteideikq rlsvenqrml delqlrdrdv enlkkqleel stmeqtmkdk lqgkeedlar
     3301 vsndlaeker rlaelsitkd aelhnlnvqi hekearieel mvlsdeeekq lkelrnvlea
     3361 reteinslkk lledkvkefe liqnvlkkdv svvgastsqq getsdeenka fasqeldlal
     3421 ymlhqrdvrc eelthelmql leerdtlqlr lsnairvnee lrrmgggsdv aprdeastsv
     3481 seepmvehps psksegpvei akeaidapig edkealalkl sqlhtvshtk dvrlrderel
     3541 rhtqqmslla hkdvlstlpp eaaarlvnan ytlsrdvqsq ssvllnwlwg kstpkvvhm
//
LOCUS       XP_034183079            3585 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605632 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183079
VERSION     XP_034183079.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327188.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183079.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3585
                     /product="uncharacterized protein LOC117605632 isoform X3"
                     /calculated_mol_wt=413577
     Region          <118..>698
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          509..1429
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1283..2040
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1874..2703
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2761..>3428
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3585
                     /gene="LOC117605632"
                     /coded_by="XM_034327188.2:262..11019"
                     /db_xref="GeneID:117605632"
ORIGIN      
        1 mwstseeppg tmegdssesm vhakekceqn knqleslkei mlknkqslkk keeevqeyar
       61 rlskiksrak lsrrskgenl pckdvshtse ettsvdisee tiddisqakt akakstllqk
      121 klaenrkvfe krnkeitetk raveekveai rqqleekdit amgfqkdqlp vapikpvmis
      181 sdimspkqie sieekenqia vlnkkilele atvvdlqenl kekdsviesk travtlmsad
      241 lskkgkttld tledtkdemr tmqehfille tslknknesl leqlqgrdnk iaeledsikg
      301 feqqlsqsad advsrstldt lletkeamks mqenfvlies slkakndhll eqledyelkl
      361 aeanerifkl esgigiikdp tvddlqykle klehsnkqlq dekyelqkni aelqdkilnl
      421 svngnngalm ekdnrivele nlieelkqsn klleeeskae lqqkladltl kneeysnkia
      481 dleglvhele eqksdiaarl sdesslkgde kvakltkele elnksmikik aqhrskiknl
      541 qkqlenvkki sdtnaelvrl gnqvalleee kasagdwqer vidleskvsa qtkeiemqie
      601 aiatlenqkl dlmqelhiak qevssleaen aesenlrvta emkivdleeq leamnklqnd
      661 nklesssedn haellkqvea ltqenaelys riskleekgt setgstesfe aiqeldksdl
      721 lkkiedlmhk nneltlkvsk yqekenshtg stewletmne rdknellkkv eqltqentdl
      781 amklsrmeek gssdtgstes feripehnes mtkielltqe nnelvikftk lkeqlentme
      841 idaklraeie vlqqadkdks selerltael rdlaeekdrl qkqieslive knelaalhtk
      901 leedrsvseq etvdfaersk rsvdveaeid eekdllekev kglerlrest qdlqidelng
      961 rieellkene rvlsklsele tyndklkenl tettrekeel qskindmsrd esakemlefe
     1021 kkdvvpekee mpqsdessqq tesmivasle reieeckkli aeqtglieem klklankeee
     1081 leekgkqlte yeasgkkaeg lenelkeifd tveewkhkci emqekmekle agkasieegf
     1141 kmlqnenkil leenkekdae tdrlkqqlqd tvttfeskvq eqlaaiseke neignlkqii
     1201 edkdqelhak ytelqnkmia idslqdecnn ckmliqernn vlasmsdeva nlnnamkske
     1261 deiytlrkhi delsekldes kplkdysell eqlknkdmil delhcrinat tkensnlsek
     1321 lkslsqhnnn iqsqlaekqr efvdmvaake hletliaetk kekdeaegri welqsvidnn
     1381 mkfvddlqge lrgsykhieq lklkhtedve lqnqrlenli eelnsktqec etlrdeleek
     1441 erlvgrnite evkvaleari vdlaeklkes eekiqsqlek mkklaanlkk kttvcqeleg
     1501 rvteleekwt tekdekeakn kqiqdveiam rekdnritdl eekltrarne nadafkniee
     1561 ltsdlsdske kitllteqie emeqeivklr adlesrtael giereskesi isdydsykqs
     1621 vireneqkqv eldevkekar elsvrmqvme aeyveqlasi knltaengll lskqtqinek
     1681 lenvekesee rrllieqmrr vtteavtest qtteeentee egktegaeqc drckqcqtlv
     1741 ralelklqer eaeienldne lansignfvr mnqnsmrnrs ledpyndllf qynslisshe
     1801 evkakldeas kenkqlieki eqlqsmnvtl eetvttteqs ieghredsln sdlakereer
     1861 eaellnlqre iqtlrervne leqqlnsqti slesvedskk dlrqeieslk sdretndkli
     1921 edlrnelegy rnqsgesvek piakreatfd qqenaprlfd askifgsfas dsdlltdnea
     1981 krlqalleek earcsnltqe mnhlqklmme ersqmsqnys qcveeleisr keltttlsnl
     2041 evlertlsek dkqidalnle lqnlnlrmme qqndtkesql eelrtslnst kealdaiive
     2101 reqrnalles yklqvnnlea elknstdsel iqelesrvsc ttkerdllql qvndlsrsme
     2161 elkysmgvel ekigrerdea kemvanltra ledaykqsmt stitdtstke stpleevprd
     2221 qlepgspatk avveeakqgt astplssevp fvddgtgwda eklvdeeswg wdaeeaqlvg
     2281 ehvtaslips memqlrarvd dledrikdme kerdrmlees kavqlrnakm ikklkeykvq
     2341 veslqqqlkt qksssdfyel dsaieeelkc qiskleksls evreelkntv aekealtkrl
     2401 dvivsanery memkerqdmd mevlrirnke lsdkiealdk klqsgvtaae dsevpraesv
     2461 vsgeedkpvp rervrkrsvd pedyeslckk yreeiddlke emealasend llqhfleeqk
     2521 vkmsslekkr taeenesiqi vddlnkrise lqamlsksre eyellrkqye qslmdandqv
     2581 tamrqntdfl keearekigk lemevadlrk qleifessna elqkhlqevf qekssleerl
     2641 tdlmassekq lssmnasmae vtdllniriq evadlkqelq rqyveheeak tklqetvegl
     2701 nqelgekrqq leslkqslse kenefvqqqs vetvsalvsq atqelvqkha ieieekekei
     2761 qsllgklsal etvvneysse kqsilarlde qqqelqllrq dlteknsslv afqtnltsil
     2821 sekeqelfdr neridrltad nqslletige lqrrlyefet tsidtsglts riqsletsll
     2881 ekesdlarca kefneyrail ennrieieql radkrdidel kseltakaeq mnrlnvelea
     2941 trkalgetrq ilnektclle kcnraleekd sessavpmve snqpsasniv dglplfrmte
     3001 dnhgfqqtid gmqaelekkq eeiehlkyvl nentypgiir emqdtincly dekaelkssl
     3061 dalnekfeek akeiesltes lkakslevvs keqtgllsre rasmqdqeri vglqnelhak
     3121 eqeinemkyv laekdsqlsl qasmepqsdd felremverl tgevygkeqe vqqlksiiae
     3181 lqkevsrlqe ferlseetkd aieklgdeke qarleaeeyl krtleqkete ideikqrlsv
     3241 enqrmldelq lrdrdvenlk kqleelstme qtmkdklqgk eedlarvsnd laekerrlae
     3301 lsitkdaelh nlnvqiheke arieelmvls deeekqlkel rnvlearete inslkklled
     3361 kvkefeliqn vlkkdvsvvg astsqqgets deenkafasq eldlalymlh qrdvrceelt
     3421 helmqlleer dtlqlrlsna irvneelrrm gggsdvaprd eastsvseep mvehpspsks
     3481 egpveiakea idapigedke alalklsqlh tvshtkdvrl rderelrhtq qmsllahkdv
     3541 lstlppeaaa rlvnanytls rdvqsqssvl lnwlwgkstp kvvhm
//
LOCUS       XP_034183087            3556 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605632 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183087
VERSION     XP_034183087.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327196.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183087.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3556
                     /product="uncharacterized protein LOC117605632 isoform X4"
                     /calculated_mol_wt=410306
     Region          28..855
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          541..1400
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1254..2011
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1845..2674
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2732..>3399
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3556
                     /gene="LOC117605632"
                     /coded_by="XM_034327196.2:262..10932"
                     /db_xref="GeneID:117605632"
ORIGIN      
        1 mwstseeppg tmegdssesm vhakekceqn knqleslkei mlknkqslkk keeevqeyar
       61 rlskiksrak lsrrskgenl pckdvshtse ettsvdisee tiddisqakt akakstllqk
      121 klaenrkvfe krnkeitetk raveekveai rqqleekdit amgfqkdqlp vapikpvmis
      181 sdimspkqie sieekenqia vlnkkilele atvvdlqenl kekdsviesk travtlmsad
      241 lskkgkttld tledtkdemr tmqehfille tslknknesl leqlqgrdnk iaeledsikg
      301 feqqlsqsad advsrstldt lletkeamks mqenfvlies slkakndhll eqledyelkl
      361 aeanerifkl esgigiikdp tvddlqykle klehsnkqlq dekyelqkni aelqdkilnl
      421 svngnngalm ekdnrivele nlieelkqsn klleeeskae lqqkladltl kneeysnkia
      481 dleglvhele eqksdiaarl sdesslkgde kvakltkele elnksmikik aqhrskiknl
      541 qkqlenvkki sdtnaelvrl gnqvalleee kgnlqlslvd fdelkelhia kqevssleae
      601 naesenlrvt aemkivdlee qleamnklqn dnklesssed nhaellkqve altqenaely
      661 sriskleekg tsetgstesf eaiqeldksd llkkiedlmh knneltlkvs kyqekensht
      721 gstewletmn erdknellkk veqltqentd lamklsrmee kgssdtgste sferipehne
      781 smtkielltq ennelvikft klkeqlentm eidaklraei evlqqadkdk sselerltae
      841 lrdlaeekdr lqkqiesliv eknelaalht kleedrsvse qetvdfaers krsvdveaei
      901 deekdlleke vkglerlres tqdlqideln grieellken ervlsklsel etyndklken
      961 ltettrekee lqskindmsr desakemlef ekkdvvpeke empqsdessq qtesmivasl
     1021 ereieeckkl iaeqtgliee mklklankee eleekgkqlt eyeasgkkae glenelkeif
     1081 dtveewkhkc iemqekmekl eagkasieeg fkmlqnenki lleenkekda etdrlkqqlq
     1141 dtvttfeskv qeqlaaisek eneignlkqi iedkdqelha kytelqnkmi aidslqdecn
     1201 nckmliqern nvlasmsdev anlnnamksk edeiytlrkh idelseklde skplkdysel
     1261 leqlknkdmi ldelhcrina ttkensnlse klkslsqhnn niqsqlaekq refvdmvaak
     1321 ehletliaet kkekdeaegr iwelqsvidn nmkfvddlqg elrgsykhie qlklkhtedv
     1381 elqnqrlenl ieelnsktqe cetlrdelee kerlvgrnit eevkvalear ivdlaeklke
     1441 seekiqsqle kmkklaanlk kkttvcqele grvteleekw ttekdekeak nkqiqdveia
     1501 mrekdnritd leekltrarn enadafknie eltsdlsdsk ekitllteqi eemeqeivkl
     1561 radlesrtae lgiereskes iisdydsykq svireneqkq veldevkeka relsvrmqvm
     1621 eaeyveqlas iknltaengl llskqtqine klenvekese errllieqmr rvtteavtes
     1681 tqtteeente eegktegaeq cdrckqcqtl vralelklqe reaeienldn elansignfv
     1741 rmnqnsmrnr sledpyndll fqynslissh eevkakldea skenkqliek ieqlqsmnvt
     1801 leetvttteq sieghredsl nsdlakeree reaellnlqr eiqtlrervn eleqqlnsqt
     1861 islesvedsk kdlrqeiesl ksdretndkl iedlrneleg yrnqsgesve kpiakreatf
     1921 dqqenaprlf daskifgsfa sdsdlltdne akrlqallee kearcsnltq emnhlqklmm
     1981 eersqmsqny sqcveeleis rkeltttlsn levlertlse kdkqidalnl elqnlnlrmm
     2041 eqqndtkesq leelrtslns tkealdaiiv ereqrnalle syklqvnnle aelknstdse
     2101 liqelesrvs cttkerdllq lqvndlsrsm eelkysmgve lekigrerde akemvanltr
     2161 aledaykqsm tstitdtstk estpleevpr dqlepgspat kavveeakqg tastplssev
     2221 pfvddgtgwd aeklvdeesw gwdaeeaqlv gehvtaslip smemqlrarv ddledrikdm
     2281 ekerdrmlee skavqlrnak mikklkeykv qveslqqqlk tqksssdfye ldsaieeelk
     2341 cqiskleksl sevreelknt vaekealtkr ldvivsaner ymemkerqdm dmevlrirnk
     2401 elsdkieald kklqsgvtaa edsevpraes vvsgeedkpv prervrkrsv dpedyeslck
     2461 kyreeiddlk eemealasen dllqhfleeq kvkmsslekk rtaeenesiq ivddlnkris
     2521 elqamlsksr eeyellrkqy eqslmdandq vtamrqntdf lkeearekig klemevadlr
     2581 kqleifessn aelqkhlqev fqekssleer ltdlmassek qlssmnasma evtdllniri
     2641 qevadlkqel qrqyveheea ktklqetveg lnqelgekrq qleslkqsls ekenefvqqq
     2701 svetvsalvs qatqelvqkh aieieekeke iqsllgklsa letvvneyss ekqsilarld
     2761 eqqqelqllr qdlteknssl vafqtnltsi lsekeqelfd rneridrlta dnqslletig
     2821 elqrrlyefe ttsidtsglt sriqsletsl lekesdlarc akefneyrai lennrieieq
     2881 lradkrdide lkseltakae qmnrlnvele atrkalgetr qilnektcll ekcnraleek
     2941 dsessavpmv esnqpsasni vdglplfrmt ednhgfqqti dgmqaelekk qeeiehlkyv
     3001 lnentypgii remqdtincl ydekaelkss ldalnekfee kakeieslte slkakslevv
     3061 skeqtgllsr erasmqdqer ivglqnelha keqeinemky vlaekdsqls lqasmepqsd
     3121 dfelremver ltgevygkeq evqqlksiia elqkevsrlq eferlseetk daieklgdek
     3181 eqarleaeey lkrtleqket eideikqrls venqrmldel qlrdrdvenl kkqleelstm
     3241 eqtmkdklqg keedlarvsn dlaekerrla elsitkdael hnlnvqihek earieelmvl
     3301 sdeeekqlke lrnvlearet einslkklle dkvkefeliq nvlkkdvsvv gastsqqget
     3361 sdeenkafas qeldlalyml hqrdvrceel thelmqllee rdtlqlrlsn airvneelrr
     3421 mgggsdvapr deastsvsee pmvehpspsk segpveiake aidapigedk ealalklsql
     3481 htvshtkdvr lrderelrht qqmsllahkd vlstlppeaa arlvnanytl srdvqsqssv
     3541 llnwlwgkst pkvvhm
//
LOCUS       XP_034183095            3542 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605632 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183095
VERSION     XP_034183095.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327204.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183095.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3542
                     /product="uncharacterized protein LOC117605632 isoform X5"
                     /calculated_mol_wt=408734
     Region          <33..>655
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          534..1386
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1240..1997
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1831..2660
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          2718..>3385
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3542
                     /gene="LOC117605632"
                     /coded_by="XM_034327204.2:262..10890"
                     /db_xref="GeneID:117605632"
ORIGIN      
        1 mwstseeppg tmegdssesm vhakekceqn knqleslkei mlknkqslkk keeevqeyar
       61 rlskiksrak lsrrskgenl pckdvshtse ettsvdisee tiddisqakt akakstllqk
      121 klaenrkvfe krnkeitetk raveekveai rqqleekdit amgfqkdqlp vapikpvmis
      181 sdimspkqie sieekenqia vlnkkilele atvvdlqenl kekdsviesk travtlmsad
      241 lskkgkttld tledtkdemr tmqehfille tslknknesl leqlqgrdnk iaeledsikg
      301 feqqlsqsad advsrstldt lletkeamks mqenfvlies slkakndhll eqledyelkl
      361 aeanerifkl esgigiikdp tvddlqykle klehsnkqlq dekyelqkni aelqdkilnl
      421 svngnngalm ekdnrivele nlieelkqsn klleeeskae lqqkladltl kneeysnkia
      481 dleglvhele eqksdiaarl sdesslkgde kvakltkele elnksmikik aqhrskiknl
      541 qkqlenvkki sdtnaelvrl gnqvalleee kelhiakqev ssleaenaes enlrvtaemk
      601 ivdleeqlea mnklqndnkl esssednhae llkqvealtq enaelysris kleekgtset
      661 gstesfeaiq eldksdllkk iedlmhknne ltlkvskyqe kenshtgste wletmnerdk
      721 nellkkveql tqentdlamk lsrmeekgss dtgstesfer ipehnesmtk ielltqenne
      781 lvikftklke qlentmeida klraeievlq qadkdkssel erltaelrdl aeekdrlqkq
      841 ieslivekne laalhtklee drsvseqetv dfaerskrsv dveaeideek dllekevkgl
      901 erlrestqdl qidelngrie ellkenervl sklseletyn dklkenltet trekeelqsk
      961 indmsrdesa kemlefekkd vvpekeempq sdessqqtes mivaslerei eeckkliaeq
     1021 tglieemklk lankeeelee kgkqlteyea sgkkaeglen elkeifdtve ewkhkciemq
     1081 ekmekleagk asieegfkml qnenkillee nkekdaetdr lkqqlqdtvt tfeskvqeql
     1141 aaisekenei gnlkqiiedk dqelhakyte lqnkmiaids lqdecnnckm liqernnvla
     1201 smsdevanln namkskedei ytlrkhidel sekldeskpl kdyselleql knkdmildel
     1261 hcrinattke nsnlseklks lsqhnnniqs qlaekqrefv dmvaakehle tliaetkkek
     1321 deaegriwel qsvidnnmkf vddlqgelrg sykhieqlkl khtedvelqn qrlenlieel
     1381 nsktqecetl rdeleekerl vgrniteevk valearivdl aeklkeseek iqsqlekmkk
     1441 laanlkkktt vcqelegrvt eleekwttek dekeaknkqi qdveiamrek dnritdleek
     1501 ltrarnenad afknieelts dlsdskekit llteqieeme qeivklradl esrtaelgie
     1561 reskesiisd ydsykqsvir eneqkqveld evkekarels vrmqvmeaey veqlasiknl
     1621 taenglllsk qtqineklen vekeseerrl lieqmrrvtt eavtestqtt eeenteeegk
     1681 tegaeqcdrc kqcqtlvral elklqereae ienldnelan signfvrmnq nsmrnrsled
     1741 pyndllfqyn slissheevk akldeasken kqliekieql qsmnvtleet vttteqsieg
     1801 hredslnsdl akereereae llnlqreiqt lrervneleq qlnsqtisle svedskkdlr
     1861 qeieslksdr etndkliedl rnelegyrnq sgesvekpia kreatfdqqe naprlfdask
     1921 ifgsfasdsd lltdneakrl qalleekear csnltqemnh lqklmmeers qmsqnysqcv
     1981 eeleisrkel tttlsnlevl ertlsekdkq idalnlelqn lnlrmmeqqn dtkesqleel
     2041 rtslnstkea ldaiivereq rnallesykl qvnnleaelk nstdseliqe lesrvscttk
     2101 erdllqlqvn dlsrsmeelk ysmgveleki grerdeakem vanltraled aykqsmtsti
     2161 tdtstkestp leevprdqle pgspatkavv eeakqgtast plssevpfvd dgtgwdaekl
     2221 vdeeswgwda eeaqlvgehv taslipsmem qlrarvddle drikdmeker drmleeskav
     2281 qlrnakmikk lkeykvqves lqqqlktqks ssdfyeldsa ieeelkcqis klekslsevr
     2341 eelkntvaek ealtkrldvi vsanerymem kerqdmdmev lrirnkelsd kiealdkklq
     2401 sgvtaaedse vpraesvvsg eedkpvprer vrkrsvdped yeslckkyre eiddlkeeme
     2461 alasendllq hfleeqkvkm sslekkrtae enesiqivdd lnkriselqa mlsksreeye
     2521 llrkqyeqsl mdandqvtam rqntdflkee arekigklem evadlrkqle ifessnaelq
     2581 khlqevfqek ssleerltdl massekqlss mnasmaevtd llniriqeva dlkqelqrqy
     2641 veheeaktkl qetveglnqe lgekrqqles lkqslseken efvqqqsvet vsalvsqatq
     2701 elvqkhaiei eekekeiqsl lgklsaletv vneyssekqs ilarldeqqq elqllrqdlt
     2761 eknsslvafq tnltsilsek eqelfdrner idrltadnqs lletigelqr rlyefettsi
     2821 dtsgltsriq sletslleke sdlarcakef neyrailenn rieieqlrad krdidelkse
     2881 ltakaeqmnr lnveleatrk algetrqiln ektcllekcn raleekdses savpmvesnq
     2941 psasnivdgl plfrmtednh gfqqtidgmq aelekkqeei ehlkyvlnen typgiiremq
     3001 dtinclydek aelkssldal nekfeekake ieslteslka kslevvskeq tgllsreras
     3061 mqdqerivgl qnelhakeqe inemkyvlae kdsqlslqas mepqsddfel remverltge
     3121 vygkeqevqq lksiiaelqk evsrlqefer lseetkdaie klgdekeqar leaeeylkrt
     3181 leqketeide ikqrlsvenq rmldelqlrd rdvenlkkql eelstmeqtm kdklqgkeed
     3241 larvsndlae kerrlaelsi tkdaelhnln vqihekeari eelmvlsdee ekqlkelrnv
     3301 leareteins lkklledkvk efeliqnvlk kdvsvvgast sqqgetsdee nkafasqeld
     3361 lalymlhqrd vrceelthel mqlleerdtl qlrlsnairv neelrrmggg sdvaprdeas
     3421 tsvseepmve hpspsksegp veiakeaida pigedkeala lklsqlhtvs htkdvrlrde
     3481 relrhtqqms llahkdvlst lppeaaarlv nanytlsrdv qsqssvllnw lwgkstpkvv
     3541 hm
//
LOCUS       XP_034183207             985 aa            linear   INV 03-SEP-2025
DEFINITION  regulator of telomere elongation helicase 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183207
VERSION     XP_034183207.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327316.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..985
                     /product="regulator of telomere elongation helicase 1
                     isoform X1"
                     /calculated_mol_wt=112181
     Region          11..724
                     /region_name="rad3"
                     /note="DNA repair helicase (rad3); TIGR00604"
                     /db_xref="CDD:273169"
     Region          878..981
                     /region_name="harmonin_N_like"
                     /note="N-terminal protein-binding module of harmonin and
                     similar domains, also known as HHD (harmonin homology
                     domain); cl11968"
                     /db_xref="CDD:448372"
     Site            order(894,906,910,923,926,929..930,933)
                     /site_type="other"
                     /note="putative protein binding site [polypeptide
                     binding]"
                     /db_xref="CDD:259826"
     CDS             1..985
                     /gene="Rtel1"
                     /coded_by="XM_034327316.2:1..2958"
                     /db_xref="GeneID:117605698"
ORIGIN      
        1 mpevtinnvi vnfpfkpysv qeeymtkvie clqnnkngvl esptgtgktl cllcsslswl
       61 ltkkaqlqaq slvagvenpt fgghffkklk nelneatgns npatsfswta pkiiyasrth
      121 sqlsqamqel krtsykhvtv avlgsrdqlc ihpevskein alnkiymcqs kvksrtcfyy
      181 nnvetrkddp sfkqevldie dlvkaghknk ccpyflskel kqnadiifmp ynylldpksr
      241 rsqgidlqns vvlldeahni ekiceeaasl qicstdiamc ideltvvmey iakdieqnef
      301 smetsgnvqi dftaedlyil ktmfldleka idsiqivkre egdthpggyi fellekaqlt
      361 hgkeqvvien lekivlyltt tstspftrkg nalqkfsdvl rtvfshgnsl rykekikqcy
      421 kvhiqveeqk kskkdvwgtk kkvsknegkv isywcfspgy smqqlmeqgv qsvvltsgtl
      481 splkpfisel gipievqlen phivkgeqic vgvlsqgpdg yslnssfntr ndpkyimslg
      541 rtifnfscli phglliffps ypvmkkcree wqnvglwtki aerkpiyvep qykddflkii
      601 neyyerikdp shkgaifmav crgkvsegld fanangravm itglpfpplk dprvilkqry
      661 leeikrqdnd vlsgqqwyql easravnqai griirhkndy gaiilcdcrf enpsfkkqls
      721 awlrpyvknf nhfgsitkel rdffryanct lpqprnvslp atsasfnttv sqmskntskp
      781 ktdtkssved nfnidlyidk nekdkkpaea kkksffeila tdskpminfn tckleenqts
      841 celtksites vrkkrkiiml pvecneylsg pstlsdanie nvnaptnsqq snaetdinst
      901 dkkvlgkmyi kevkqslsqs nykifskiiq dynstnnfee llkvlqnlfp pnkgldhlfi
      961 gfkhflkkqh iaafevqvah lrdmq
//
LOCUS       XP_034183217             961 aa            linear   INV 03-SEP-2025
DEFINITION  regulator of telomere elongation helicase 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183217
VERSION     XP_034183217.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327326.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..961
                     /product="regulator of telomere elongation helicase 1
                     isoform X2"
                     /calculated_mol_wt=109341
     Region          1..700
                     /region_name="rad3"
                     /note="DNA repair helicase (rad3); TIGR00604"
                     /db_xref="CDD:273169"
     Region          854..957
                     /region_name="harmonin_N_like"
                     /note="N-terminal protein-binding module of harmonin and
                     similar domains, also known as HHD (harmonin homology
                     domain); cl11968"
                     /db_xref="CDD:448372"
     Site            order(870,882,886,899,902,905..906,909)
                     /site_type="other"
                     /note="putative protein binding site [polypeptide
                     binding]"
                     /db_xref="CDD:259826"
     CDS             1..961
                     /gene="Rtel1"
                     /coded_by="XM_034327326.2:118..3003"
                     /db_xref="GeneID:117605698"
ORIGIN      
        1 mtkvieclqn nkngvlespt gtgktlcllc sslswlltkk aqlqaqslva gvenptfggh
       61 ffkklkneln eatgnsnpat sfswtapkii yasrthsqls qamqelkrts ykhvtvavlg
      121 srdqlcihpe vskeinalnk iymcqskvks rtcfyynnve trkddpsfkq evldiedlvk
      181 aghknkccpy flskelkqna diifmpynyl ldpksrrsqg idlqnsvvll deahniekic
      241 eeaaslqics tdiamcidel tvvmeyiakd ieqnefsmet sgnvqidfta edlyilktmf
      301 ldlekaidsi qivkreegdt hpggyifell ekaqlthgke qvvienleki vlyltttsts
      361 pftrkgnalq kfsdvlrtvf shgnslryke kikqcykvhi qveeqkkskk dvwgtkkkvs
      421 knegkvisyw cfspgysmqq lmeqgvqsvv ltsgtlsplk pfiselgipi evqlenphiv
      481 kgeqicvgvl sqgpdgysln ssfntrndpk yimslgrtif nfscliphgl liffpsypvm
      541 kkcreewqnv glwtkiaerk piyvepqykd dflkiineyy erikdpshkg aifmavcrgk
      601 vsegldfana ngravmitgl pfpplkdprv ilkqryleei krqdndvlsg qqwyqleasr
      661 avnqaigrii rhkndygaii lcdcrfenps fkkqlsawlr pyvknfnhfg sitkelrdff
      721 ryanctlpqp rnvslpatsa sfnttvsqms kntskpktdt kssvednfni dlyidknekd
      781 kkpaeakkks ffeilatdsk pminfntckl eenqtscelt ksitesvrkk rkiimlpvec
      841 neylsgpstl sdanienvna ptnsqqsnae tdinstdkkv lgkmyikevk qslsqsnyki
      901 fskiiqdyns tnnfeellkv lqnlfppnkg ldhlfigfkh flkkqhiaaf evqvahlrdm
      961 q
//
LOCUS       XP_034183230             726 aa            linear   INV 03-SEP-2025
DEFINITION  arylsulfatase B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183230
VERSION     XP_034183230.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183230.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..726
                     /product="arylsulfatase B isoform X1"
                     /calculated_mol_wt=81472
     Region          91..469
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(99..100,138,192,194,286,349..350)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(99..100,138,192,194,286,350)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     CDS             1..726
                     /gene="LOC117605706"
                     /coded_by="XM_034327339.2:329..2509"
                     /db_xref="GeneID:117605706"
ORIGIN      
        1 menlswiikf attilpflhl fgfgmnvawi ttlltvvfll efllvanvdg iffpsllsss
       61 kvktnfpttg klgnedakve dgnrqdskek phiviiladd mgwndvsfhg sdqiptpnid
      121 alayngiiln nhyvpalctp srtalmtgkn pihlgmqhsv llpaeprglp lsekllpeyl
      181 keigyrthav gkwhlgyfrk eytptyrgfd shygywnglq dyythitqeq disdfrgfdm
      241 rrnltvawet qgkystdlft neavrlikeh dtnqpmflyl ahlaphkgnp nqllrasdkd
      301 iakfsyildp erriqaavis kldqsvgevi dalrnrgmlk nsivlfmsdn gaptegvlsn
      361 qgsnyplrgi kdspweggtr gvaaiwspli keprrvsnqm mfmadwfptl lsaagaerkh
      421 lgnidgfdlw palisdkisp rsdivlnidd isnysairrg dfkyvigqte tgrawlgatg
      481 nssegvspky dpfkvlyskv gvaisgvitv kqamelderr knvkngtnip lktdfqakil
      541 tadkllelrn narircnvke enkipcnpte apclfniekd pcemvnlaer rplilailer
      601 vltnyrltmv ppsnvpddpk anpllwnntw tswddpnpla lafkineyht rsgpaiavms
      661 iicglfivgv itlfalkcdk snsndtedhd yengqetavr tanydsdslt mskiiedakr
      721 dsedrn
//
LOCUS       XP_076546028             716 aa            linear   INV 03-SEP-2025
DEFINITION  arylsulfatase B isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546028
VERSION     XP_076546028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689913.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..716
                     /product="arylsulfatase B isoform X2"
                     /calculated_mol_wt=80098
     Region          81..459
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(89..90,128,182,184,276,339..340)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(89..90,128,182,184,276,340)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     CDS             1..716
                     /gene="LOC117605706"
                     /coded_by="XM_076689913.1:162..2312"
                     /db_xref="GeneID:117605706"
ORIGIN      
        1 mtiiiaitvs vgfgmnvawi ttlltvvfll efllvanvdg iffpsllsss kvktnfpttg
       61 klgnedakve dgnrqdskek phiviiladd mgwndvsfhg sdqiptpnid alayngiiln
      121 nhyvpalctp srtalmtgkn pihlgmqhsv llpaeprglp lsekllpeyl keigyrthav
      181 gkwhlgyfrk eytptyrgfd shygywnglq dyythitqeq disdfrgfdm rrnltvawet
      241 qgkystdlft neavrlikeh dtnqpmflyl ahlaphkgnp nqllrasdkd iakfsyildp
      301 erriqaavis kldqsvgevi dalrnrgmlk nsivlfmsdn gaptegvlsn qgsnyplrgi
      361 kdspweggtr gvaaiwspli keprrvsnqm mfmadwfptl lsaagaerkh lgnidgfdlw
      421 palisdkisp rsdivlnidd isnysairrg dfkyvigqte tgrawlgatg nssegvspky
      481 dpfkvlyskv gvaisgvitv kqamelderr knvkngtnip lktdfqakil tadkllelrn
      541 narircnvke enkipcnpte apclfniekd pcemvnlaer rplilailer vltnyrltmv
      601 ppsnvpddpk anpllwnntw tswddpnpla lafkineyht rsgpaiavms iicglfivgv
      661 itlfalkcdk snsndtedhd yengqetavr tanydsdslt mskiiedakr dsedrn
//
LOCUS       XP_034183239             702 aa            linear   INV 03-SEP-2025
DEFINITION  arylsulfatase B isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034183239
VERSION     XP_034183239.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327348.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183239.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..702
                     /product="arylsulfatase B isoform X3"
                     /calculated_mol_wt=78694
     Region          67..445
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(75..76,114,168,170,262,325..326)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(75..76,114,168,170,262,326)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     CDS             1..702
                     /gene="LOC117605706"
                     /coded_by="XM_034327348.2:165..2273"
                     /db_xref="GeneID:117605706"
ORIGIN      
        1 mnvawittll tvvfllefll vanvdgiffp sllssskvkt nfpttgklgn edakvedgnr
       61 qdskekphiv iiladdmgwn dvsfhgsdqi ptpnidalay ngiilnnhyv palctpsrta
      121 lmtgknpihl gmqhsvllpa eprglplsek llpeylkeig yrthavgkwh lgyfrkeytp
      181 tyrgfdshyg ywnglqdyyt hitqeqdisd frgfdmrrnl tvawetqgky stdlftneav
      241 rlikehdtnq pmflylahla phkgnpnqll rasdkdiakf syildperri qaaviskldq
      301 svgevidalr nrgmlknsiv lfmsdngapt egvlsnqgsn yplrgikdsp weggtrgvaa
      361 iwsplikepr rvsnqmmfma dwfptllsaa gaerkhlgni dgfdlwpali sdkisprsdi
      421 vlniddisny sairrgdfky vigqtetgra wlgatgnsse gvspkydpfk vlyskvgvai
      481 sgvitvkqam elderrknvk ngtniplktd fqakiltadk llelrnnari rcnvkeenki
      541 pcnpteapcl fniekdpcem vnlaerrpli lailervltn yrltmvppsn vpddpkanpl
      601 lwnntwtswd dpnplalafk ineyhtrsgp aiavmsiicg lfivgvitlf alkcdksnsn
      661 dtedhdyeng qetavrtany dsdsltmski iedakrdsed rn
//
LOCUS       XP_034183247             702 aa            linear   INV 03-SEP-2025
DEFINITION  arylsulfatase B isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034183247
VERSION     XP_034183247.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327356.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183247.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..702
                     /product="arylsulfatase B isoform X3"
                     /calculated_mol_wt=78694
     Region          67..445
                     /region_name="4-S"
                     /note="N-acetylgalactosamine 4-sulfatase, also called
                     arylsulftase B; cd16029"
                     /db_xref="CDD:293753"
     Site            order(75..76,114,168,170,262,325..326)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293753"
     Site            order(75..76,114,168,170,262,326)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293753"
     CDS             1..702
                     /gene="LOC117605706"
                     /coded_by="XM_034327356.2:38..2146"
                     /db_xref="GeneID:117605706"
ORIGIN      
        1 mnvawittll tvvfllefll vanvdgiffp sllssskvkt nfpttgklgn edakvedgnr
       61 qdskekphiv iiladdmgwn dvsfhgsdqi ptpnidalay ngiilnnhyv palctpsrta
      121 lmtgknpihl gmqhsvllpa eprglplsek llpeylkeig yrthavgkwh lgyfrkeytp
      181 tyrgfdshyg ywnglqdyyt hitqeqdisd frgfdmrrnl tvawetqgky stdlftneav
      241 rlikehdtnq pmflylahla phkgnpnqll rasdkdiakf syildperri qaaviskldq
      301 svgevidalr nrgmlknsiv lfmsdngapt egvlsnqgsn yplrgikdsp weggtrgvaa
      361 iwsplikepr rvsnqmmfma dwfptllsaa gaerkhlgni dgfdlwpali sdkisprsdi
      421 vlniddisny sairrgdfky vigqtetgra wlgatgnsse gvspkydpfk vlyskvgvai
      481 sgvitvkqam elderrknvk ngtniplktd fqakiltadk llelrnnari rcnvkeenki
      541 pcnpteapcl fniekdpcem vnlaerrpli lailervltn yrltmvppsn vpddpkanpl
      601 lwnntwtswd dpnplalafk ineyhtrsgp aiavmsiicg lfivgvitlf alkcdksnsn
      661 dtedhdyeng qetavrtany dsdsltmski iedakrdsed rn
//
LOCUS       XP_034183273             541 aa            linear   INV 03-SEP-2025
DEFINITION  threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034183273
VERSION     XP_034183273.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327382.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183273.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..541
                     /product="threonylcarbamoyladenosine tRNA
                     methylthiotransferase isoform X1"
                     /calculated_mol_wt=61272
     Region          62..488
                     /region_name="MiaB-like-B"
                     /note="MiaB-like tRNA modifying enzyme, archaeal-type;
                     TIGR01578"
                     /db_xref="CDD:273703"
     CDS             1..541
                     /gene="LOC117605727"
                     /coded_by="XM_034327382.2:252..1877"
                     /db_xref="GeneID:117605727"
ORIGIN      
        1 mlapcqdiie diedlissqd itpkeryssr knvtfrpkrm qlheelpqpp vfdsivpgtq
       61 tiyvktwgct hnnsdseyma gqlaaygyrl tedkhkadlw llnsctvksp sedhfrneie
      121 vgrkqgkhiv lagcvpqgap kssflqglsv igvqqidrvv evveetlkgn tvrflqqkke
      181 agkkvggasl rlpkvrrnpl ieiiaintgc lnqctycktk hargelgsyp peeiveratq
      241 afnegvcelw ltsedtgtyg rdigtslpel lwklievvpd gcmirlgmtn ppyilehlee
      301 mskilrhpkv ysflhvpvqs gsdqvladmk reytcadfem vvnflrervp gltiatdiic
      361 gfpteteqdf eetmklcerh kfpslfinqf fprpgtpaar mqkvptqdvk krtkrlseff
      421 qsyfpyddnr vgiiqdvlvt eishdkkhyv ghnksyeqil vplkegylgk mikvkilett
      481 kysmkgeptn egispsvkqc lpkgtvsgip iemkpskyri aailtifvav flrllwkfln
      541 v
//
LOCUS       XP_034183282             502 aa            linear   INV 03-SEP-2025
DEFINITION  threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034183282
VERSION     XP_034183282.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327391.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183282.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="threonylcarbamoyladenosine tRNA
                     methylthiotransferase isoform X2"
                     /calculated_mol_wt=56696
     Region          23..449
                     /region_name="MiaB-like-B"
                     /note="MiaB-like tRNA modifying enzyme, archaeal-type;
                     TIGR01578"
                     /db_xref="CDD:273703"
     CDS             1..502
                     /gene="LOC117605727"
                     /coded_by="XM_034327391.2:279..1787"
                     /db_xref="GeneID:117605727"
ORIGIN      
        1 mqlheelpqp pvfdsivpgt qtiyvktwgc thnnsdseym agqlaaygyr ltedkhkadl
       61 wllnsctvks psedhfrnei evgrkqgkhi vlagcvpqga pkssflqgls vigvqqidrv
      121 vevveetlkg ntvrflqqkk eagkkvggas lrlpkvrrnp lieiiaintg clnqctyckt
      181 khargelgsy ppeeiverat qafnegvcel wltsedtgty grdigtslpe llwklievvp
      241 dgcmirlgmt nppyilehle emskilrhpk vysflhvpvq sgsdqvladm kreytcadfe
      301 mvvnflrerv pgltiatdii cgfpteteqd feetmklcer hkfpslfinq ffprpgtpaa
      361 rmqkvptqdv kkrtkrlsef fqsyfpyddn rvgiiqdvlv teishdkkhy vghnksyeqi
      421 lvplkegylg kmikvkilet tkysmkgept negispsvkq clpkgtvsgi piemkpskyr
      481 iaailtifva vflrllwkfl nv
//
LOCUS       XP_034183294             507 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 4aa1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183294
VERSION     XP_034183294.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327403.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183294.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="putative cytochrome P450 4aa1 isoform X1"
                     /calculated_mol_wt=58957
     Region          77..499
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(108,125..126,133,137,144,310,313..314,317,378,382,
                     443..445,449..453,456..457,461)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(126,312..313,317,381,383)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..507
                     /gene="Cyp4aa1"
                     /coded_by="XM_034327403.2:1619..3142"
                     /db_xref="GeneID:117605740"
ORIGIN      
        1 mvvtaqqiwd qtpggvwtyl iilasvyily ilrtyirtvi fvsrlngpkt vpflgnarcv
       61 lkdnllhrla henqtygriv riwltglpyv iliepediqi vlssmkhtrk iffyklldnf
      121 lgkglitrdv dtwrthrkfl qpafhlhile rftstfaeca dhlmnqflnk nnqeinitsf
      181 indsvydils etvlginras rqsglimddd lpfrkgqiml lyrmvrpwll iewiyrltkh
      241 grqeekqrkd lfntcfkmmk enrdllrnkd invnetknik rtslleymve mneknpcftd
      301 ediveecctf mlagqdsvgt atamtiflla nhpewqekci eeldnifngs srlpamkdlk
      361 emrclemcik eslrlypsvp iiarilgedv kigkhvvpag cgvfispyst hrlphhfsdp
      421 eafkperfns ensekrhpya yipfsagprn cignkfamle mksmisailr kcrlesipgk
      481 kevrpkfrmt iraqgglwvk vvvrdqk
//
LOCUS       XP_034183313             504 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 4aa1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183313
VERSION     XP_034183313.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327422.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183313.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..504
                     /product="putative cytochrome P450 4aa1 isoform X2"
                     /calculated_mol_wt=58671
     Region          74..496
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(105,122..123,130,134,141,307,310..311,314,375,379,
                     440..442,446..450,453..454,458)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(123,309..310,314,378,380)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..504
                     /gene="Cyp4aa1"
                     /coded_by="XM_034327422.2:654..2168"
                     /db_xref="GeneID:117605740"
ORIGIN      
        1 mvlikwdqtp ggvwtyliil asvyilyilr tyirtvifvs rlngpktvpf lgnarcvlkd
       61 nllhrlahen qtygrivriw ltglpyvili epediqivls smkhtrkiff yklldnflgk
      121 glitrdvdtw rthrkflqpa fhlhilerft stfaecadhl mnqflnknnq einitsfind
      181 svydilsetv lginrasrqs glimdddlpf rkgqimllyr mvrpwlliew iyrltkhgrq
      241 eekqrkdlfn tcfkmmkenr dllrnkdinv netknikrts lleymvemne knpcftdedi
      301 veecctfmla gqdsvgtata mtifllanhp ewqekcieel dnifngssrl pamkdlkemr
      361 clemcikesl rlypsvpiia rilgedvkig khvvpagcgv fispysthrl phhfsdpeaf
      421 kperfnsens ekrhpyayip fsagprncig nkfamlemks misailrkcr lesipgkkev
      481 rpkfrmtira qgglwvkvvv rdqk
//
LOCUS       XP_034183321             504 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 4aa1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183321
VERSION     XP_034183321.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327430.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183321.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..504
                     /product="putative cytochrome P450 4aa1 isoform X2"
                     /calculated_mol_wt=58671
     Region          74..496
                     /region_name="CYP4"
                     /note="cytochrome P450 family 4; cd20628"
                     /db_xref="CDD:410721"
     Site            order(105,122..123,130,134,141,307,310..311,314,375,379,
                     440..442,446..450,453..454,458)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410721"
     Site            order(123,309..310,314,378,380)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410721"
     CDS             1..504
                     /gene="Cyp4aa1"
                     /coded_by="XM_034327430.2:114..1628"
                     /db_xref="GeneID:117605740"
ORIGIN      
        1 mvlikwdqtp ggvwtyliil asvyilyilr tyirtvifvs rlngpktvpf lgnarcvlkd
       61 nllhrlahen qtygrivriw ltglpyvili epediqivls smkhtrkiff yklldnflgk
      121 glitrdvdtw rthrkflqpa fhlhilerft stfaecadhl mnqflnknnq einitsfind
      181 svydilsetv lginrasrqs glimdddlpf rkgqimllyr mvrpwlliew iyrltkhgrq
      241 eekqrkdlfn tcfkmmkenr dllrnkdinv netknikrts lleymvemne knpcftdedi
      301 veecctfmla gqdsvgtata mtifllanhp ewqekcieel dnifngssrl pamkdlkemr
      361 clemcikesl rlypsvpiia rilgedvkig khvvpagcgv fispysthrl phhfsdpeaf
      421 kperfnsens ekrhpyayip fsagprncig nkfamlemks misailrkcr lesipgkkev
      481 rpkfrmtira qgglwvkvvv rdqk
//
LOCUS       XP_034183335             476 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605758 [Osmia lignaria lignaria].
ACCESSION   XP_034183335
VERSION     XP_034183335.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327444.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="uncharacterized protein LOC117605758"
                     /calculated_mol_wt=53422
     Region          277..320
                     /region_name="F-box_EMI"
                     /note="F-box domain found in the early mitotic inhibitor
                     (EMI) family of F-box proteins; cd22086"
                     /db_xref="CDD:438858"
     Site            order(283,286..287,290..291,295,297..298,302..304,306)
                     /site_type="other"
                     /note="putative Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438858"
     Region          401..449
                     /region_name="BRcat_RBR_EMI"
                     /note="BRcat domain found in early mitotic inhibitor (EMI)
                     subfamily of F-box proteins; cd20348"
                     /db_xref="CDD:439009"
     CDS             1..476
                     /gene="LOC117605758"
                     /coded_by="XM_034327444.2:539..1969"
                     /db_xref="GeneID:117605758"
ORIGIN      
        1 mslrnskhss hdsvdsgylt ltprsgaytp ilsgyrsrvs cisskrhprv rvdplqsera
       61 qrhrsklnyl lntsefeyta pesseresfs qetssfsddr pslsieqsgs eltnqsstec
      121 hefedgfrrr trrsykttvp itpastrlql lspppspavp spfiasepie kdmemklvnv
      181 pqstpqvtts prrktppptp riiisptcrk asptissadn qklsklsikd lsaiaeikpk
      241 rldfshrais valyrtratp dytgretvdi lallgeksnh wrivskilsy lgpqdlcsis
      301 mvsktwrric indsrantkr lthiilrqnv kenlklikks kmegniqtsp ksryarkgyl
      361 levqnllqaq gnqrppsspp vspskvkfhs fvkasrtlap wehllpcpkc sfpchvdgek
      421 nvgtcsrqgc smefctfcss rphtgpcktp llatptkrnk rlvvgsrqsk rnlrrl
//
LOCUS       XP_034183350             465 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605759 [Osmia lignaria lignaria].
ACCESSION   XP_034183350
VERSION     XP_034183350.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327459.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183350.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..465
                     /product="uncharacterized protein LOC117605759"
                     /calculated_mol_wt=51667
     Region          141..294
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          345..>398
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..465
                     /gene="LOC117605759"
                     /coded_by="XM_034327459.2:569..1966"
                     /db_xref="GeneID:117605759"
ORIGIN      
        1 mdssassrtv lrpmanlint psmkqsvcal ctsghfqypa krsfqrrqsa ilkglcfgtm
       61 gphllkncht pdrcgicqkh hhtssnlvks vsfsfpssns khisgipere ltvlnasrpl
      121 csssrpniir rtpvllatal iivvsengeh fharalldqg sevsfitell aemlgltrrf
      181 savpiigiga qhsdvargat sfqlvsrvde sftchvdali lpelttslpa alifrtqwsh
      241 leglsladpd fskpskidvi lgarvysqii reglrrgsgs apiaqqtslg wvlsgpidat
      301 lmepdrldes svhelqcspd ykslekatic dqqptskesh lgvllnrgpk lrmnltnilv
      361 rwrwyayevs tdiekiyhqt rdkeddrplq qnlwrendav sdsfqpyivi tsgdleersv
      421 mcsttdinct svvrdlvqry ssltkllrvt awcgrvgnrf rrlls
//
LOCUS       XP_034183382             411 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605779 [Osmia lignaria lignaria].
ACCESSION   XP_034183382
VERSION     XP_034183382.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327491.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="uncharacterized protein LOC117605779"
                     /calculated_mol_wt=47925
     Region          41..333
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..411
                     /gene="LOC117605779"
                     /coded_by="XM_034327491.2:384..1619"
                     /db_xref="GeneID:117605779"
ORIGIN      
        1 msnsdtelke vvlrdlepvl hqkidnklti enyttksllp pgenygssif svhvelknek
       61 tgkkeelqli akmvpptefq rrlfnssrtf mkeifmydti ipaynklele cgfkeselfd
      121 itakfygsrl ssqpgtdfdd navilmdnlk vkgyytgdrs kgydlehael tvkalarfha
      181 lgiatkikkp gifevfkmya kslqgesigg smfklilski kedpemnpyf dkcykiftev
      241 tidniwndil repwttiihs dfwvnnvmfr rnekgavddv kfidfqiyly gsplrdllff
      301 lfssveleit deeienlmdq yyvafiktle kmgcktdvft kegfkekmhe daerefvhlc
      361 fmvkiltlsv gdiddfnydk mqnvmieykg selfierlrr vvlsfvkrnw i
//
LOCUS       XP_034183394             363 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-tubulin N-acetyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034183394
VERSION     XP_034183394.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327503.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..363
                     /product="alpha-tubulin N-acetyltransferase"
                     /calculated_mol_wt=41018
     Region          8..187
                     /region_name="Acetyltransf_16"
                     /note="GNAT acetyltransferase, Mec-17; pfam05301"
                     /db_xref="CDD:461616"
     CDS             1..363
                     /gene="LOC117605786"
                     /coded_by="XM_034327503.2:811..1902"
                     /db_xref="GeneID:117605786"
ORIGIN      
        1 mefkfnvnkl lprkinkvth tlipedfrgd rrelhecqrq lsrilddmge asaraqglnk
       61 sitnslklrd tdhivyllvd neannelgsv vgllktgskn lfmfdeigah yqlkprcild
      121 fyvhesrqrm glgnilyqhm lseenirpvk laidrpsekf lafldkyygl skiipqnnkf
      181 vvfqgffdde rqdvrtnrys lpanatmdig asntmhnnfg knnssmngvp ysssvsrasf
      241 gryaaarppc smaniihnta vlgpsaertg eklstpaate skperprsls lyseeeqkqr
      301 tsemstvptp alpehqptpf vtilretekt eknikpspsc sqevagtnqh arldlkfyhs
      361 plw
//
LOCUS       XP_034183406             340 aa            linear   INV 03-SEP-2025
DEFINITION  fatty acid hydroxylase domain-containing protein 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183406
VERSION     XP_034183406.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327515.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="fatty acid hydroxylase domain-containing protein
                     2"
                     /calculated_mol_wt=39166
     Region          181..305
                     /region_name="FA_hydroxylase"
                     /note="Fatty acid hydroxylase; pfam04116"
                     /db_xref="CDD:397991"
     CDS             1..340
                     /gene="LOC117605792"
                     /coded_by="XM_034327515.2:243..1265"
                     /db_xref="GeneID:117605792"
ORIGIN      
        1 mmdvqsrykv iparsdgckn qtpptllssl rsvlfvigtv vigfaafcns ltwhlqrfwg
       61 asgdfwqaqw dkildklgdd pvtlwvygsl gltffvywlf ggiytildlt nrpaalrryk
      121 iqpgtnepvn tkdlckviaq vlfnqiivgl playvsyhfm ewrgyppvre lptfhwvlve
      181 iaihilceei gfyyshrflh srylykyihk qhhewtapia vtslychple higsnllppf
      241 lgvfivgshv atawlwfsla ilstlnahsg yhlpffpspe ahdfhhlkfn qcfgvlgvld
      301 rihgtdtqfr nsrayqrhvi mlslvpprea fpdqvkykfk
//
LOCUS       XP_034183418             299 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605798 [Osmia lignaria lignaria].
ACCESSION   XP_034183418
VERSION     XP_034183418.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327527.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183418.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..299
                     /product="uncharacterized protein LOC117605798"
                     /calculated_mol_wt=32619
     Region          <39..299
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..299
                     /gene="LOC117605798"
                     /coded_by="XM_034327527.2:269..1168"
                     /db_xref="GeneID:117605798"
ORIGIN      
        1 mglfvmqnfh giilfiwivs iltlsksdvv ktdgtaplts alnatsvhtl pkneyqtlps
       61 svnstsasvv ptktsnfqkn vatpsvageg gpisipsttq dmtsfhspnv skeentqnte
      121 nrkntkivsr kganemlntk qenvadvkvt tfndtklise sssaasiphn nekpidlntn
      181 alklnttiln ntlnaanitq emlsksttvt assvqehrpk ptatvvgpns dkrafishtk
      241 glhlgmskki nyvfpvivtl ialpilgaii fmiykqgrdc wdkrhyrrmd flidgmynd
//
LOCUS       XP_034183428             285 aa            linear   INV 03-SEP-2025
DEFINITION  glucosamine-6-phosphate isomerase Oscillin isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183428
VERSION     XP_034183428.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327537.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="glucosamine-6-phosphate isomerase Oscillin
                     isoform X1"
                     /calculated_mol_wt=32272
     Region          1..253
                     /region_name="SugarP_isomerase"
                     /note="Sugar Phosphate Isomerase family; includes type A
                     ribose 5-phosphate isomerase (RPI_A),
                     glucosamine-6-phosphate (GlcN6P) deaminase, and
                     6-phosphogluconolactonase (6PGL). RPI catalyzes the
                     reversible conversion of ribose-5-phosphate to
                     ribulose...; cl00339"
                     /db_xref="CDD:469729"
     Site            order(41..44,71..72,137..138,143,145,172,208)
                     /site_type="active"
                     /db_xref="CDD:238693"
     Site            order(150,152..153,161,216..222,230..232)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:238693"
     Site            order(151..152,158..161)
                     /site_type="active"
                     /note="allosteric site [active]"
                     /db_xref="CDD:238693"
     Site            order(163,166..176,180..182)
                     /site_type="active"
                     /note="active site lid [active]"
                     /db_xref="CDD:238693"
     Site            order(168,206,210,241,244..247)
                     /site_type="other"
                     /note="hexamer (dimer of trimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:238693"
     CDS             1..285
                     /gene="Oscillin"
                     /coded_by="XM_034327537.2:339..1196"
                     /db_xref="GeneID:117605808"
ORIGIN      
        1 mrlvicdtvd hvaewsakyv lkrindfqpn kdkyfvlglp tggtplgmyk klieyhkegk
       61 lsfqyvktfn mdeyvdlprd hpesyhyymy nnffkhidin pdnvhildgn alnlvqecdd
      121 fekkikdagg ielfiggigp dghiafnepg sslvsrtrvk tlaqdtlqan arffgndisk
      181 vpkqaltvgv gtvmdakevm ilitgshkaf alykaieegi nhmwtvsafq qhprtiivcd
      241 edatlelrvk tvkyfkalsa vhhklieedg daiprcsiqn ylskw
//
LOCUS       XP_034183434             285 aa            linear   INV 03-SEP-2025
DEFINITION  glucosamine-6-phosphate isomerase Oscillin isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183434
VERSION     XP_034183434.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327543.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="glucosamine-6-phosphate isomerase Oscillin
                     isoform X1"
                     /calculated_mol_wt=32272
     Region          1..253
                     /region_name="SugarP_isomerase"
                     /note="Sugar Phosphate Isomerase family; includes type A
                     ribose 5-phosphate isomerase (RPI_A),
                     glucosamine-6-phosphate (GlcN6P) deaminase, and
                     6-phosphogluconolactonase (6PGL). RPI catalyzes the
                     reversible conversion of ribose-5-phosphate to
                     ribulose...; cl00339"
                     /db_xref="CDD:469729"
     Site            order(41..44,71..72,137..138,143,145,172,208)
                     /site_type="active"
                     /db_xref="CDD:238693"
     Site            order(150,152..153,161,216..222,230..232)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:238693"
     Site            order(151..152,158..161)
                     /site_type="active"
                     /note="allosteric site [active]"
                     /db_xref="CDD:238693"
     Site            order(163,166..176,180..182)
                     /site_type="active"
                     /note="active site lid [active]"
                     /db_xref="CDD:238693"
     Site            order(168,206,210,241,244..247)
                     /site_type="other"
                     /note="hexamer (dimer of trimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:238693"
     CDS             1..285
                     /gene="Oscillin"
                     /coded_by="XM_034327543.2:184..1041"
                     /db_xref="GeneID:117605808"
ORIGIN      
        1 mrlvicdtvd hvaewsakyv lkrindfqpn kdkyfvlglp tggtplgmyk klieyhkegk
       61 lsfqyvktfn mdeyvdlprd hpesyhyymy nnffkhidin pdnvhildgn alnlvqecdd
      121 fekkikdagg ielfiggigp dghiafnepg sslvsrtrvk tlaqdtlqan arffgndisk
      181 vpkqaltvgv gtvmdakevm ilitgshkaf alykaieegi nhmwtvsafq qhprtiivcd
      241 edatlelrvk tvkyfkalsa vhhklieedg daiprcsiqn ylskw
//
LOCUS       XP_034183452             285 aa            linear   INV 03-SEP-2025
DEFINITION  glucosamine-6-phosphate isomerase Oscillin isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183452
VERSION     XP_034183452.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327561.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="glucosamine-6-phosphate isomerase Oscillin
                     isoform X1"
                     /calculated_mol_wt=32272
     Region          1..253
                     /region_name="SugarP_isomerase"
                     /note="Sugar Phosphate Isomerase family; includes type A
                     ribose 5-phosphate isomerase (RPI_A),
                     glucosamine-6-phosphate (GlcN6P) deaminase, and
                     6-phosphogluconolactonase (6PGL). RPI catalyzes the
                     reversible conversion of ribose-5-phosphate to
                     ribulose...; cl00339"
                     /db_xref="CDD:469729"
     Site            order(41..44,71..72,137..138,143,145,172,208)
                     /site_type="active"
                     /db_xref="CDD:238693"
     Site            order(150,152..153,161,216..222,230..232)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:238693"
     Site            order(151..152,158..161)
                     /site_type="active"
                     /note="allosteric site [active]"
                     /db_xref="CDD:238693"
     Site            order(163,166..176,180..182)
                     /site_type="active"
                     /note="active site lid [active]"
                     /db_xref="CDD:238693"
     Site            order(168,206,210,241,244..247)
                     /site_type="other"
                     /note="hexamer (dimer of trimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:238693"
     CDS             1..285
                     /gene="Oscillin"
                     /coded_by="XM_034327561.2:339..1196"
                     /db_xref="GeneID:117605808"
ORIGIN      
        1 mrlvicdtvd hvaewsakyv lkrindfqpn kdkyfvlglp tggtplgmyk klieyhkegk
       61 lsfqyvktfn mdeyvdlprd hpesyhyymy nnffkhidin pdnvhildgn alnlvqecdd
      121 fekkikdagg ielfiggigp dghiafnepg sslvsrtrvk tlaqdtlqan arffgndisk
      181 vpkqaltvgv gtvmdakevm ilitgshkaf alykaieegi nhmwtvsafq qhprtiivcd
      241 edatlelrvk tvkyfkalsa vhhklieedg daiprcsiqn ylskw
//
LOCUS       XP_034183443             285 aa            linear   INV 03-SEP-2025
DEFINITION  glucosamine-6-phosphate isomerase Oscillin isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183443
VERSION     XP_034183443.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327552.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="glucosamine-6-phosphate isomerase Oscillin
                     isoform X1"
                     /calculated_mol_wt=32272
     Region          1..253
                     /region_name="SugarP_isomerase"
                     /note="Sugar Phosphate Isomerase family; includes type A
                     ribose 5-phosphate isomerase (RPI_A),
                     glucosamine-6-phosphate (GlcN6P) deaminase, and
                     6-phosphogluconolactonase (6PGL). RPI catalyzes the
                     reversible conversion of ribose-5-phosphate to
                     ribulose...; cl00339"
                     /db_xref="CDD:469729"
     Site            order(41..44,71..72,137..138,143,145,172,208)
                     /site_type="active"
                     /db_xref="CDD:238693"
     Site            order(150,152..153,161,216..222,230..232)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:238693"
     Site            order(151..152,158..161)
                     /site_type="active"
                     /note="allosteric site [active]"
                     /db_xref="CDD:238693"
     Site            order(163,166..176,180..182)
                     /site_type="active"
                     /note="active site lid [active]"
                     /db_xref="CDD:238693"
     Site            order(168,206,210,241,244..247)
                     /site_type="other"
                     /note="hexamer (dimer of trimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:238693"
     CDS             1..285
                     /gene="Oscillin"
                     /coded_by="XM_034327552.2:400..1257"
                     /db_xref="GeneID:117605808"
ORIGIN      
        1 mrlvicdtvd hvaewsakyv lkrindfqpn kdkyfvlglp tggtplgmyk klieyhkegk
       61 lsfqyvktfn mdeyvdlprd hpesyhyymy nnffkhidin pdnvhildgn alnlvqecdd
      121 fekkikdagg ielfiggigp dghiafnepg sslvsrtrvk tlaqdtlqan arffgndisk
      181 vpkqaltvgv gtvmdakevm ilitgshkaf alykaieegi nhmwtvsafq qhprtiivcd
      241 edatlelrvk tvkyfkalsa vhhklieedg daiprcsiqn ylskw
//
LOCUS       XP_034183462             284 aa            linear   INV 03-SEP-2025
DEFINITION  glucosamine-6-phosphate isomerase Oscillin isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183462
VERSION     XP_034183462.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327571.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="glucosamine-6-phosphate isomerase Oscillin
                     isoform X2"
                     /calculated_mol_wt=32086
     Region          1..253
                     /region_name="SugarP_isomerase"
                     /note="Sugar Phosphate Isomerase family; includes type A
                     ribose 5-phosphate isomerase (RPI_A),
                     glucosamine-6-phosphate (GlcN6P) deaminase, and
                     6-phosphogluconolactonase (6PGL). RPI catalyzes the
                     reversible conversion of ribose-5-phosphate to
                     ribulose...; cl00339"
                     /db_xref="CDD:469729"
     Site            order(41..44,71..72,137..138,143,145,172,208)
                     /site_type="active"
                     /db_xref="CDD:238693"
     Site            order(150,152..153,161,216..222,230..232)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:238693"
     Site            order(151..152,158..161)
                     /site_type="active"
                     /note="allosteric site [active]"
                     /db_xref="CDD:238693"
     Site            order(163,166..176,180..182)
                     /site_type="active"
                     /note="active site lid [active]"
                     /db_xref="CDD:238693"
     Site            order(168,206,210,241,244..247)
                     /site_type="other"
                     /note="hexamer (dimer of trimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:238693"
     CDS             1..284
                     /gene="Oscillin"
                     /coded_by="XM_034327571.2:339..1193"
                     /db_xref="GeneID:117605808"
ORIGIN      
        1 mrlvicdtvd hvaewsakyv lkrindfqpn kdkyfvlglp tggtplgmyk klieyhkegk
       61 lsfqyvktfn mdeyvdlprd hpesyhyymy nnffkhidin pdnvhildgn alnlvqecdd
      121 fekkikdagg ielfiggigp dghiafnepg sslvsrtrvk tlaqdtlqan arffgndisk
      181 vpkqaltvgv gtvmdakevm ilitgshkaf alykaieegi nhmwtvsafq qhprtiivcd
      241 edatlelrvk tvkyfkalsa vhhklieedg daiprcsiqn ylsk
//
LOCUS       XP_034183486             256 aa            linear   INV 03-SEP-2025
DEFINITION  farnesol dehydrogenase-like [Osmia lignaria lignaria].
ACCESSION   XP_034183486
VERSION     XP_034183486.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327595.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="farnesol dehydrogenase-like"
                     /calculated_mol_wt=27463
     Region          1..239
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(13,15..16,18,37..39,89..91,140..142,158,162,
                     190..193)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(113,142,158,162)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..256
                     /gene="LOC117605840"
                     /coded_by="XM_034327595.2:1..771"
                     /db_xref="GeneID:117605840"
ORIGIN      
        1 mnqwcgkiaa vtgassgigk atsiallkhg vnvvglarrl etmkkipnev egikgkfypi
       61 qcdlskeedi ikafkfvetl ggadilvnna glgyvesite sstehmknvl dlnvlasaic
      121 areitqslrk rkargqiinv nsvlghnaal iskpcslyet skyaitgmse tlrkdmqqsk
      181 apikvtslsp givktdlsll qdaggetffd avshiysedi adgiiyvltt pphvqvnelt
      241 italhsgiip tekikl
//
LOCUS       XP_034183501             256 aa            linear   INV 03-SEP-2025
DEFINITION  farnesol dehydrogenase-like [Osmia lignaria lignaria].
ACCESSION   XP_034183501
VERSION     XP_034183501.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327610.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="farnesol dehydrogenase-like"
                     /calculated_mol_wt=27479
     Region          1..238
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(13,15..16,18,37..39,88..90,139..141,157,161,
                     189..192)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(112,141,157,161)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..256
                     /gene="LOC117605847"
                     /coded_by="XM_034327610.2:40..810"
                     /db_xref="GeneID:117605847"
ORIGIN      
        1 mdrwngkvai vtgassgige aiseglvkrg vkvvglarrv dklqeltskl gkdkfyfvqc
       61 dlrkeedllk afkwvekelg gadilvnnag tvqlvnvvds tteeyykvid tnliapaica
      121 retvqslrkr nasghiinin siaghfaesl htplgmyaas kygltalste lrheiiagnl
      181 sikvtsvspg lvntemvsky lpadkkipil qaqdvadaal yalgtpehve ipelmiiphg
      241 tpiggnadsp vtprnp
//
LOCUS       XP_034183512             255 aa            linear   INV 03-SEP-2025
DEFINITION  farnesol dehydrogenase-like [Osmia lignaria lignaria].
ACCESSION   XP_034183512
VERSION     XP_034183512.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327621.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183512.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="farnesol dehydrogenase-like"
                     /calculated_mol_wt=27349
     Region          1..238
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(13,15..16,18,37..39,88..90,139..141,157,161,
                     189..192)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187601"
     Site            order(112,141,157,161)
                     /site_type="active"
                     /db_xref="CDD:187601"
     CDS             1..255
                     /gene="LOC117605852"
                     /coded_by="XM_034327621.2:83..850"
                     /db_xref="GeneID:117605852"
ORIGIN      
        1 mnrwngkvai vtgassgige ataealvkhg ikvvglarrv dklkdlatkl gkdkfypiqc
       61 dlrkeddilk amkwvekelg gadilvnnag vaittpvids ateeyykvid tnliaptica
      121 revirclkkr ntaghvinin siaghfaesl hmplgmyaps kygltalgse lrheiivanl
      181 nikvtsispg avetemasgi ippnlgipvl kaediaaaii yavgtpehve ipeitimphr
      241 atigipttfd qmkkk
//
LOCUS       XP_034183524             255 aa            linear   INV 03-SEP-2025
DEFINITION  farnesol dehydrogenase-like [Osmia lignaria lignaria].
ACCESSION   XP_034183524
VERSION     XP_034183524.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327633.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="farnesol dehydrogenase-like"
                     /calculated_mol_wt=27578
     Region          1..238
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(13,15..16,18,37..39,88..90,139..141,157,161,
                     189..192)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(112,141,157,161)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..255
                     /gene="LOC117605856"
                     /coded_by="XM_034327633.2:295..1062"
                     /db_xref="GeneID:117605856"
ORIGIN      
        1 mdrwfgkvai vtgassgige tivealmkhg ikvvgiarsv nrlreltvkf gkdkfypmec
       61 dltkeedilk afkwiekelg gadilvnsag alikipiids ptenfhkvid tnliapaica
      121 retiqslkkr naaghiinin svcghfaesv hiplgiypas kygvtaltve lrheiiaanl
      181 nvkvtsispg vvktnmlkhn ipidlatptl qtadvaeaii yalqtpehae lieitlmphg
      241 lavgcpllpe lmkel
//
LOCUS       XP_034183538             217 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L10Ab [Osmia lignaria lignaria].
ACCESSION   XP_034183538
VERSION     XP_034183538.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327647.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="ribosomal protein L10Ab"
                     /calculated_mol_wt=24479
     Region          3..217
                     /region_name="Ribosomal_L1"
                     /note="Ribosomal protein L1. The L1 protein, located near
                     the E-site of the ribosome, forms part of the L1 stalk
                     along with 23S rRNA. In bacteria and archaea, L1 functions
                     both as a ribosomal protein that binds rRNA, and as a
                     translation repressor that binds...; cl00322"
                     /db_xref="CDD:469720"
     Site            order(26..28,31,33,35,37,162,164,166,210..211,213)
                     /site_type="other"
                     /note="mRNA/rRNA interface [nucleotide binding]"
                     /db_xref="CDD:238235"
     CDS             1..217
                     /gene="RpL10Ab"
                     /coded_by="XM_034327647.2:146..799"
                     /db_xref="GeneID:117605864"
ORIGIN      
        1 mtskvsrdtl yecvnsviqn sqdkkrkfle tvelqiglkn ydpqkdkrfs gtvklknipr
       61 pkmqvcvlgd qqhcdeakan nipymdaeal kklnknkklv kklakkydaf laseslikqi
      121 privgpglnk agkfpgllsh qesmvgkide vkatikfqmk kvlclsvavg hvemtpdelv
      181 qnvhlsinfl vsllkkhwqn vrslhikssm gppqrly
//
LOCUS       XP_034183550             212 aa            linear   INV 03-SEP-2025
DEFINITION  polyisoprenoid diphosphate/phosphate phosphohydrolase PLPP6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183550
VERSION     XP_034183550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327659.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..212
                     /product="polyisoprenoid diphosphate/phosphate
                     phosphohydrolase PLPP6"
                     /calculated_mol_wt=24087
     Region          45..184
                     /region_name="PAP2_containing_2_like"
                     /note="PAP2, subfamily similar to human
                     phosphatidic_acid_phosphatase_type_2_domain_containing_2.
                     PAP2 is a super-family of phosphatases and
                     haloperoxidases. This subgroup, which is specific to
                     eukaryota, lacks functional characterization and may act
                     as a...; cd03391"
                     /db_xref="CDD:239485"
     Site            order(94,101,120..122,158,164,168)
                     /site_type="active"
                     /db_xref="CDD:239485"
     CDS             1..212
                     /gene="LOC117605868"
                     /coded_by="XM_034327659.2:169..807"
                     /db_xref="GeneID:117605868"
ORIGIN      
        1 mgkhhkreip pmlknilaid vqlteklvkc ieklmplrql kihntaleis chgipwiasi
       61 lafiwildnk nlyqmqvnll fgllldiivi ailkaffrrr rpvptndpfs vgpdkysfps
      121 ghvsrsvlif yvfkylwpvs vicllpiaaw ifslamsrll mrrhhildic aglilgyaeg
      181 mllsflylda ktcaylvswl ndakvdgaed da
//
LOCUS       XP_034195518             649 aa            linear   INV 03-SEP-2025
DEFINITION  F-box and WD-40 domain protein 7 [Osmia lignaria lignaria].
ACCESSION   XP_034195518
VERSION     XP_034195518.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339627.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="F-box and WD-40 domain protein 7"
                     /calculated_mol_wt=72069
     Region          203..261
                     /region_name="F-box_FBXW7"
                     /note="F-box domain found in F-box/WD repeat-containing
                     protein 7 (FBXW7) and similar proteins; cd22133"
                     /db_xref="CDD:438905"
     Site            order(216..219,222..224,227,230..232,234..235,238..239,
                     241..248,250..251)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438905"
     Region          <307..595
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          326..361
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          367..401
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          406..440
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          447..480
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          486..520
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          528..560
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          569..591
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..649
                     /gene="ago"
                     /coded_by="XM_034339627.2:370..2319"
                     /db_xref="GeneID:117611614"
ORIGIN      
        1 msvpssssnk isngkpggts pspvegiegv pgpsslepmh sllpqdeese dygedgeeee
       61 ydsseeesei sdtgmfcdae cepdlesnnc slsttqsqsl tqqvhspilh tcvpldvvqp
      121 qrkrksetes slpckklnpe dnkshsmivk kldekgkhmr cviptkdnpp pemsnwllqf
      181 qrwsnaerml aidelierce ptqvrhmmqv iepqfqrdfi sllpkelals vlafleprdl
      241 lraaqtcrnw rfladdnllw kekcraagie dlkdlppvkr kncrnsgnss spwkqaymrq
      301 hnikmnwrtk pirtpkvlkg hddhvitclq fsgnrivsgs ddntlkvwsa itgkclrtlv
      361 ghtggvwssq msgttvisgs tdrtlkvwna etgqcihtly ghtstvrcmh lhgnkvvsgs
      421 rdatlrvwqv dtgeclhvlv ghlaavrcvq ydgklvvsga ydymvkvwnp ereeclhtlq
      481 ghtnrvyslq fdgvhvvsgs ldtsirvwev etgacrhtlm ghqsltsgme lrnnilvsgn
      541 adstvkvwdi vsghclqtls gpnkhqsavt clqfnshfvi tssddgtvkl wdvktgdfir
      601 nlvalesggs ggvvwriras dtklvcavgs rngteetkll vldfdvevk
//
LOCUS       XP_034195526             649 aa            linear   INV 03-SEP-2025
DEFINITION  F-box and WD-40 domain protein 7 [Osmia lignaria lignaria].
ACCESSION   XP_034195526
VERSION     XP_034195526.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339635.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="F-box and WD-40 domain protein 7"
                     /calculated_mol_wt=72069
     Region          203..261
                     /region_name="F-box_FBXW7"
                     /note="F-box domain found in F-box/WD repeat-containing
                     protein 7 (FBXW7) and similar proteins; cd22133"
                     /db_xref="CDD:438905"
     Site            order(216..219,222..224,227,230..232,234..235,238..239,
                     241..248,250..251)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438905"
     Region          <307..595
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          326..361
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          367..401
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          406..440
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          447..480
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          486..520
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          528..560
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          569..591
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..649
                     /gene="ago"
                     /coded_by="XM_034339635.2:455..2404"
                     /db_xref="GeneID:117611614"
ORIGIN      
        1 msvpssssnk isngkpggts pspvegiegv pgpsslepmh sllpqdeese dygedgeeee
       61 ydsseeesei sdtgmfcdae cepdlesnnc slsttqsqsl tqqvhspilh tcvpldvvqp
      121 qrkrksetes slpckklnpe dnkshsmivk kldekgkhmr cviptkdnpp pemsnwllqf
      181 qrwsnaerml aidelierce ptqvrhmmqv iepqfqrdfi sllpkelals vlafleprdl
      241 lraaqtcrnw rfladdnllw kekcraagie dlkdlppvkr kncrnsgnss spwkqaymrq
      301 hnikmnwrtk pirtpkvlkg hddhvitclq fsgnrivsgs ddntlkvwsa itgkclrtlv
      361 ghtggvwssq msgttvisgs tdrtlkvwna etgqcihtly ghtstvrcmh lhgnkvvsgs
      421 rdatlrvwqv dtgeclhvlv ghlaavrcvq ydgklvvsga ydymvkvwnp ereeclhtlq
      481 ghtnrvyslq fdgvhvvsgs ldtsirvwev etgacrhtlm ghqsltsgme lrnnilvsgn
      541 adstvkvwdi vsghclqtls gpnkhqsavt clqfnshfvi tssddgtvkl wdvktgdfir
      601 nlvalesggs ggvvwriras dtklvcavgs rngteetkll vldfdvevk
//
LOCUS       XP_034195533             649 aa            linear   INV 03-SEP-2025
DEFINITION  F-box and WD-40 domain protein 7 [Osmia lignaria lignaria].
ACCESSION   XP_034195533
VERSION     XP_034195533.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339642.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="F-box and WD-40 domain protein 7"
                     /calculated_mol_wt=72069
     Region          203..261
                     /region_name="F-box_FBXW7"
                     /note="F-box domain found in F-box/WD repeat-containing
                     protein 7 (FBXW7) and similar proteins; cd22133"
                     /db_xref="CDD:438905"
     Site            order(216..219,222..224,227,230..232,234..235,238..239,
                     241..248,250..251)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438905"
     Region          <307..595
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          326..361
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          367..401
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          406..440
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          447..480
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          486..520
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          528..560
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          569..591
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..649
                     /gene="ago"
                     /coded_by="XM_034339642.2:132..2081"
                     /db_xref="GeneID:117611614"
ORIGIN      
        1 msvpssssnk isngkpggts pspvegiegv pgpsslepmh sllpqdeese dygedgeeee
       61 ydsseeesei sdtgmfcdae cepdlesnnc slsttqsqsl tqqvhspilh tcvpldvvqp
      121 qrkrksetes slpckklnpe dnkshsmivk kldekgkhmr cviptkdnpp pemsnwllqf
      181 qrwsnaerml aidelierce ptqvrhmmqv iepqfqrdfi sllpkelals vlafleprdl
      241 lraaqtcrnw rfladdnllw kekcraagie dlkdlppvkr kncrnsgnss spwkqaymrq
      301 hnikmnwrtk pirtpkvlkg hddhvitclq fsgnrivsgs ddntlkvwsa itgkclrtlv
      361 ghtggvwssq msgttvisgs tdrtlkvwna etgqcihtly ghtstvrcmh lhgnkvvsgs
      421 rdatlrvwqv dtgeclhvlv ghlaavrcvq ydgklvvsga ydymvkvwnp ereeclhtlq
      481 ghtnrvyslq fdgvhvvsgs ldtsirvwev etgacrhtlm ghqsltsgme lrnnilvsgn
      541 adstvkvwdi vsghclqtls gpnkhqsavt clqfnshfvi tssddgtvkl wdvktgdfir
      601 nlvalesggs ggvvwriras dtklvcavgs rngteetkll vldfdvevk
//
LOCUS       XP_034192547             474 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192547
VERSION     XP_034192547.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336656.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="neurotrimin isoform X1"
                     /calculated_mol_wt=53941
     Region          87..180
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          97..101
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          110..114
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          145..149
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          159..164
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          171..174
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          182..273
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          201..205
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          214..218
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          238..242
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          252..257
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          266..269
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          288..376
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          294..298
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409390"
     Region          307..311
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409390"
     Region          330..348
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409390"
     Region          358..363
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409390"
     CDS             1..474
                     /gene="LOC117609900"
                     /coded_by="XM_034336656.2:81..1505"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 mtitihliil wttvivvaav hrsngvqthg lnldyrrihq dhgrrrqsrn leilgqisgh
       61 hhgtqyasms ehyknnsdlp rfaeeiqnvt vsrgrdalla civdnlrnyq vawvrvdtqt
      121 ilsihhkvit qnprisltyn dhrtwylhik kveevdrgwy mcqvntdpmr srqgylqvvv
      181 ppsintkets tdmvvregsn vtltckatgy pepyvmwrre dgkninyngv kvnvvdgevl
      241 hivkisrlhm gaylciasnd vpprvsqris lrvqfppmls iqnqlvgayi gqdvtlecht
      301 eaypssinyw ktergdmivs gnyhsivgdk yeavytddgy nkymslkirn vgpkdfgsyk
      361 cvaqnsiget dgdikldeip apsttsttpp pyyvtssmkk nsrngnkklr qrpidyevee
      421 wrdpdydrdy gppsmrppge lpvdalspgv shraqlvfhl lvsllsllfp airr
//
LOCUS       XP_034192548             467 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192548
VERSION     XP_034192548.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336657.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="neurotrimin isoform X2"
                     /calculated_mol_wt=53171
     Region          87..180
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          97..101
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          110..114
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          145..149
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          159..164
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          171..174
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          182..273
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          201..205
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          214..218
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          238..242
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          252..257
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          266..269
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          288..369
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          294..298
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:143207"
     Region          307..312
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:143207"
     Region          337..341
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:143207"
     Region          351..356
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:143207"
     Region          364..367
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:143207"
     CDS             1..467
                     /gene="LOC117609900"
                     /coded_by="XM_034336657.2:80..1483"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 mtitihliil wttvivvaav hrsngvqthg lnldyrrihq dhgrrrqsrn leilgqisgh
       61 hhgtqyasms ehyknnsdlp rfaeeiqnvt vsrgrdalla civdnlrnyq vawvrvdtqt
      121 ilsihhkvit qnprisltyn dhrtwylhik kveevdrgwy mcqvntdpmr srqgylqvvv
      181 ppsintkets tdmvvregsn vtltckatgy pepyvmwrre dgkninyngv kvnvvdgevl
      241 hivkisrlhm gaylciasnd vpprvsqris lrvqfppmls iqnqlvgayi gqdvtlecht
      301 eaypssinyw ktergdmivs gdkyeavytd dgynkymslk irnvgpkdfg sykcvaqnsi
      361 getdgdikld eipapsttst tpppyyvtss mkknsrngnk klrqrpidye veewrdpdyd
      421 rdygppsmrp pgelpvdals pgvshraqlv fhllvsllsl lfpairr
//
LOCUS       XP_034192549             456 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034192549
VERSION     XP_034192549.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336658.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192549.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..456
                     /product="neurotrimin isoform X3"
                     /calculated_mol_wt=51716
     Region          69..162
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          79..83
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          92..96
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          127..131
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          141..146
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          153..156
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          164..255
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          183..187
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          196..200
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          220..224
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          234..239
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          248..251
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          270..358
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          276..280
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          289..293
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          312..330
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          340..345
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..456
                     /gene="LOC117609900"
                     /coded_by="XM_034336658.2:54..1424"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 mllnrifvsa tyivaqflla cpafvegrqs rnleilgqis ghhhgtqyas msehyknnsd
       61 lprfaeeiqn vtvsrgrdal lacivdnlrn yqvawvrvdt qtilsihhkv itqnprislt
      121 yndhrtwylh ikkveevdrg wymcqvntdp mrsrqgylqv vvppsintke tstdmvvreg
      181 snvtltckat gypepyvmwr redgkninyn gvkvnvvdge vlhivkisrl hmgaylcias
      241 ndvpprvsqr islrvqfppm lsiqnqlvga yigqdvtlec hteaypssin ywktergdmi
      301 vsgnyhsivg dkyeavytdd gynkymslki rnvgpkdfgs ykcvaqnsig etdgdiklde
      361 ipapsttstt pppyyvtssm kknsrngnkk lrqrpidyev eewrdpdydr dygppsmrpp
      421 gelpvdalsp gvshraqlvf hllvsllsll fpairr
//
LOCUS       XP_076546031             451 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546031
VERSION     XP_076546031.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689916.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..451
                     /product="neurotrimin isoform X4"
                     /calculated_mol_wt=51072
     Region          87..180
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          97..101
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          110..114
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          145..149
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          159..164
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          171..174
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          182..273
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          201..205
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          214..218
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          238..242
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          252..257
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          266..269
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          288..376
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          294..298
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          307..311
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          330..348
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          358..363
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..451
                     /gene="LOC117609900"
                     /coded_by="XM_076689916.1:82..1437"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 mtitihliil wttvivvaav hrsngvqthg lnldyrrihq dhgrrrqsrn leilgqisgh
       61 hhgtqyasms ehyknnsdlp rfaeeiqnvt vsrgrdalla civdnlrnyq vawvrvdtqt
      121 ilsihhkvit qnprisltyn dhrtwylhik kveevdrgwy mcqvntdpmr srqgylqvvv
      181 ppsintkets tdmvvregsn vtltckatgy pepyvmwrre dgkninyngv kvnvvdgevl
      241 hivkisrlhm gaylciasnd vpprvsqris lrvqfppmls iqnqlvgayi gqdvtlecht
      301 eaypssinyw ktergdmivs gnyhsivgdk yeavytddgy nkymslkirn vgpkdfgsyk
      361 cvaqnsiget dgdikldeip apsttsttpp pyyvtssmkk nnydrdygpp smrppgelpv
      421 dalspgvshr aqlvfhllvs llsllfpair r
//
LOCUS       XP_034192550             444 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034192550
VERSION     XP_034192550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336659.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..444
                     /product="neurotrimin isoform X5"
                     /calculated_mol_wt=50455
     Region          87..180
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          97..101
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          110..114
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          145..149
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          159..164
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          171..174
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          182..273
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          201..205
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          214..218
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          238..242
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          252..257
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          266..269
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          288..376
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          294..298
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409390"
     Region          307..311
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409390"
     Region          330..348
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409390"
     Region          358..363
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409390"
     CDS             1..444
                     /gene="LOC117609900"
                     /coded_by="XM_034336659.2:82..1416"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 mtitihliil wttvivvaav hrsngvqthg lnldyrrihq dhgrrrqsrn leilgqisgh
       61 hhgtqyasms ehyknnsdlp rfaeeiqnvt vsrgrdalla civdnlrnyq vawvrvdtqt
      121 ilsihhkvit qnprisltyn dhrtwylhik kveevdrgwy mcqvntdpmr srqgylqvvv
      181 ppsintkets tdmvvregsn vtltckatgy pepyvmwrre dgkninyngv kvnvvdgevl
      241 hivkisrlhm gaylciasnd vpprvsqris lrvqfppmls iqnqlvgayi gqdvtlecht
      301 eaypssinyw ktergdmivs gnyhsivgdk yeavytddgy nkymslkirn vgpkdfgsyk
      361 cvaqnsiget dgdikldeip apsttsttpp pyyvtssmkk nsrngnkklr qrpidyevee
      421 wrdpaqgprs lnpilnakis tscn
//
LOCUS       XP_034192551             437 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034192551
VERSION     XP_034192551.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..437
                     /product="neurotrimin isoform X6"
                     /calculated_mol_wt=49868
     Region          87..180
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          97..101
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          110..114
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          145..149
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          159..164
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          171..174
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          182..273
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          201..205
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          214..218
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          238..242
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          252..257
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          266..269
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          288..376
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          294..298
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409390"
     Region          307..311
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409390"
     Region          330..348
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409390"
     Region          358..363
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409390"
     CDS             1..437
                     /gene="LOC117609900"
                     /coded_by="XM_034336660.2:81..1394"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 mtitihliil wttvivvaav hrsngvqthg lnldyrrihq dhgrrrqsrn leilgqisgh
       61 hhgtqyasms ehyknnsdlp rfaeeiqnvt vsrgrdalla civdnlrnyq vawvrvdtqt
      121 ilsihhkvit qnprisltyn dhrtwylhik kveevdrgwy mcqvntdpmr srqgylqvvv
      181 ppsintkets tdmvvregsn vtltckatgy pepyvmwrre dgkninyngv kvnvvdgevl
      241 hivkisrlhm gaylciasnd vpprvsqris lrvqfppmls iqnqlvgayi gqdvtlecht
      301 eaypssinyw ktergdmivs gnyhsivgdk yeavytddgy nkymslkirn vgpkdfgsyk
      361 cvaqnsiget dgdikldeip apsttsttpp pyyvtssmkk nsrngnkklr qrpidyevee
      421 wrdpdnnhhr gvigihq
//
LOCUS       XP_034192552             433 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034192552
VERSION     XP_034192552.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336661.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="neurotrimin isoform X7"
                     /calculated_mol_wt=49261
     Region          87..180
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          97..101
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          110..114
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          145..149
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          159..164
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          171..174
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          182..273
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          201..205
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          214..218
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          238..242
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          252..257
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          266..269
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          288..376
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          294..298
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409390"
     Region          307..311
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409390"
     Region          330..348
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409390"
     Region          358..363
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409390"
     CDS             1..433
                     /gene="LOC117609900"
                     /coded_by="XM_034336661.2:81..1382"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 mtitihliil wttvivvaav hrsngvqthg lnldyrrihq dhgrrrqsrn leilgqisgh
       61 hhgtqyasms ehyknnsdlp rfaeeiqnvt vsrgrdalla civdnlrnyq vawvrvdtqt
      121 ilsihhkvit qnprisltyn dhrtwylhik kveevdrgwy mcqvntdpmr srqgylqvvv
      181 ppsintkets tdmvvregsn vtltckatgy pepyvmwrre dgkninyngv kvnvvdgevl
      241 hivkisrlhm gaylciasnd vpprvsqris lrvqfppmls iqnqlvgayi gqdvtlecht
      301 eaypssinyw ktergdmivs gnyhsivgdk yeavytddgy nkymslkirn vgpkdfgsyk
      361 cvaqnsiget dgdikldeip apsttsttpp pyyvtssmkk nsrngnkklr qrpidyevee
      421 wrdpvtglit ssi
//
LOCUS       XP_034192553             406 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034192553
VERSION     XP_034192553.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336662.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="neurotrimin isoform X8"
                     /calculated_mol_wt=46175
     Region          19..112
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          29..33
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409498"
     Region          42..46
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409498"
     Region          77..81
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409498"
     Region          91..96
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409498"
     Region          103..106
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409498"
     Region          114..205
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          133..137
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409562"
     Region          146..150
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409562"
     Region          170..174
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409562"
     Region          184..189
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409562"
     Region          198..201
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409562"
     Region          220..308
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          226..230
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409390"
     Region          239..243
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409390"
     Region          262..280
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409390"
     Region          290..295
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409390"
     CDS             1..406
                     /gene="LOC117609900"
                     /coded_by="XM_034336662.2:423..1643"
                     /db_xref="GeneID:117609900"
ORIGIN      
        1 msehyknnsd lprfaeeiqn vtvsrgrdal lacivdnlrn yqvawvrvdt qtilsihhkv
       61 itqnprislt yndhrtwylh ikkveevdrg wymcqvntdp mrsrqgylqv vvppsintke
      121 tstdmvvreg snvtltckat gypepyvmwr redgkninyn gvkvnvvdge vlhivkisrl
      181 hmgaylcias ndvpprvsqr islrvqfppm lsiqnqlvga yigqdvtlec hteaypssin
      241 ywktergdmi vsgnyhsivg dkyeavytdd gynkymslki rnvgpkdfgs ykcvaqnsig
      301 etdgdiklde ipapsttstt pppyyvtssm kknsrngnkk lrqrpidyev eewrdpdydr
      361 dygppsmrpp gelpvdalsp gvshraqlvf hllvsllsll fpairr
//
LOCUS       XP_034192558             557 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609909 [Osmia lignaria lignaria].
ACCESSION   XP_034192558
VERSION     XP_034192558.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336667.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192558.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="uncharacterized protein LOC117609909"
                     /calculated_mol_wt=64753
     CDS             1..557
                     /gene="LOC117609909"
                     /coded_by="XM_034336667.2:1432..3105"
                     /db_xref="GeneID:117609909"
ORIGIN      
        1 mierlqlekd lstglknslt ariarfkslr aelhgsvkig egqrrtdaes hasssssliw
       61 edvesafhnr iitgiiinte hieprnfled arkivfqrla evfqnhscik vntvlygeft
      121 vnnksdvksf svrnkqlfst snlndwydky vvnstltlme efqerdsgwa iskilhlmln
      181 inkcnpmhag cytdipktik dkravvnvtv dddtcfvrsi vaalypvivn anrpssypny
      241 dtvlnlegia fpitlnqipk ferqndvsin vyfwdlnvkr caplqlttnk rfrhvnllml
      301 emlnrnvrhf awiknlsrlv snqlsayksk tyicdrclhy fyaeemllkh tkdcmkmndc
      361 aiilpssedk iikfdnfchr erhpfivyad fecmlkkvns ninqghsnay qqyeaysvey
      421 yfhcsynasv ceyraylgpe caewfvnele nlvrkiaplf ntivpmtnlt gderesymna
      481 thcyicekpf nidetkvrdh chftgryqep ahqgcnlnyt nkrfipivfh nlsgydahfv
      541 ikefvtiaqs nkfelfl
//
LOCUS       XP_034195574            1597 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611649 [Osmia lignaria lignaria].
ACCESSION   XP_034195574
VERSION     XP_034195574.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339683.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195574.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1597
                     /product="uncharacterized protein LOC117611649"
                     /calculated_mol_wt=180286
     CDS             1..1597
                     /gene="LOC117611649"
                     /coded_by="XM_034339683.2:262..5055"
                     /db_xref="GeneID:117611649"
ORIGIN      
        1 mdreqrgrgs rasstsslgr evrcgcqyyq sdsllperra llgrppsrtm qqppavrcse
       61 heyepiaaps pspkaasapv vritdtdvvp vadsddsidd rarlppeltr agelilpldg
      121 kiedtamear elvetgdtse eletpqqlqe rleerffnap tpgaesrtdg evntsqvdsl
      181 aleelplrrg arglphrlkt qagklrtrlk nikrptfalp erqraertrs dksrtekrps
      241 rmdrirssfs erprfnlpnr prfslpdrpk fhlperpkfn lknalartrr ssppnerstd
      301 stvgsrgkni fdfstyprif erksknksdy yatstpkdsr aqsaesatfp rprkgmvvsk
      361 rwtrrfettt ayidedrsga dtaaersppw rhpsreeprl slgfegdtri pweekerpee
      421 lqymdyeqes peiserhahp tseasrtedd sydreqtesn prlyqdqdaa eaylkgtkke
      481 eveegevdwr aeeriardef ravqrsrsle avlrareeeg fvmvdprryg vhktpseeee
      541 ydeemeeeee eeeeeeaeps saqsdreqqq ssgsscdrrr rgvieeidyd efflreggls
      601 qddtnlgkyl tseirdalra eqshilvgda smpappqrpt rsrslgkqke gsiesyeamp
      661 pvrpkrdpnr srrsesieeq fpertrtdsk hrivyqtege ppelpiepld divvvkpvrr
      721 kskssqrsrs qirtdtprpp spasvppvvp trrkrlrket pterrngela aicnghtewa
      781 atdsapekpl plpptdqlpd dqeaytaeei iatseenirm latrlreeey mepapvppkr
      841 rsrsrgtsva qdedrtshga eslpeigyve ediprddldf grdlsgyaii ekrdkpprpp
      901 pprrkkdkfa ttpkpvapkr pqraystlgp smsremnilt defetttlqp aesapyieid
      961 sddgehkdlr sdevlskmqg rplpapprpp rvrkdrsler sagavnqesi metttatqtd
     1021 plpddmviee evtqaklvva psvsgsqimv steripspsn astppvvppl pisqrmrree
     1081 etelpydtvs lkspsparem enlqdrhlsa phlgeriree nprvpsvhsa llssnepvri
     1141 snlevgdlrv drlnvsqiea gkivasevda lvitaselks mtgmlaegls psiikeliai
     1201 rshletvaas qaqqsqvrev rpieeeterk pgaeqtvqeg padeeksrpt eeqkvvkrev
     1261 vetkdkemsg trtqsiaran vedneipvth tlsvtqvltt qspsvvqpte dkslrifdse
     1321 sllslstakq anveesrasl ppssrttktd ddamksrfss drmsepsdeq lesmvpaefs
     1381 tlmtehrded kessperper ksrksrsrsp spirvsparq taspvrslpp aisvtpdtpd
     1441 nvalhgitse tqpqravisy tsyteevser esqressqsp ivpsfplggt hliafpasqi
     1501 psqfysltsq pvdseasvad sarqlirvlr lacmkamrhf vrytasmvge esrekireve
     1561 lticamlllv tglliflfss trtvthhhhw dyfnppk
//
LOCUS       XP_034195587            1290 aa            linear   INV 03-SEP-2025
DEFINITION  peroxidasin [Osmia lignaria lignaria].
ACCESSION   XP_034195587
VERSION     XP_034195587.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339696.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1290
                     /product="peroxidasin"
                     /calculated_mol_wt=146097
     Region          <56..>203
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          58..81
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          82..105
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          106..129
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          130..153
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          154..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          236..326
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          253..257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          266..270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          289..293
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          303..308
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          331..417
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          347..351
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          360..364
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          382..387
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          397..402
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          424..497
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          755..1194
                     /region_name="peroxidasin_like"
                     /note="Animal heme peroxidase domain of peroxidasin and
                     related proteins; cd09826"
                     /db_xref="CDD:188658"
     Site            order(794,1063,1066)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:188658"
     Site            order(798,802,804)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:188658"
     Site            order(868,871,958,961..962,965,968,993,1045,1052)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:188658"
     Site            order(922,925..926,930,957,961,963,1119,1123,1127..1128,
                     1131..1132,1135)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:188658"
     CDS             1..1290
                     /gene="Pxn"
                     /coded_by="XM_034339696.2:2031..5903"
                     /db_xref="GeneID:117611656"
ORIGIN      
        1 mrlqlivvsl ilvilrvswa vqsqngrtec phkcmcfgst vrcmfqklnr vprvptnttv
       61 ldlrfnniae lrpgsfynlp elhtlllndn rirhlpprif egapklrily lyknrleris
      121 pgafaglpkl eqlylhynhl reikkgtfnd lpslerlflh snmlhhipad afhnvgpmtr
      181 lrldsnalvc dcnlvwllhr lqskssemaa icqapvemkg rslttmspdd fhctkprimn
      241 gpedvevrlg gtitfacevi gdpapeikwm rdsnevspdg nryviqgdgt liisdaterd
      301 ngeyecvars emgytksrka raiitvspsl rftqlpesqt vqvgtdvyfk crvegqptpe
      361 iqwwrngqtl nvggrisied dgnllriiav kesdsaryvc qarnsngeae tsadlrvvdt
      421 syspprmtye phdmeaepga iievpcrveg vpkpviqwkk dgtavegerv risrggslyl
      481 ynvtaadtgr yecsamnqyg rataqalvrv rqpeatdvlv irafkdatee idrainntli
      541 slftgdssrv npfrlarfpd avgraaarpa evfertlvni rrminagisa nttnefryeq
      601 vltaeqvrei erlsgctghr hkqnctnmcy htkyrsidgs cnnlrhptwg ssytgfrrvl
      661 qpiyengfss pvgwekgrry ygypkpaarl isttlvathn vtsderithm vmqwgqfldh
      721 dldhslpsvs seswdgidck kscdnaapcf pmdvppgdpr vtnrrcidfi rtsavcgsga
      781 tsllwgsftp reqlnqltsy ldasqvygyd dalakdlrdf ttdhgllreg ptipghkpll
      841 pyangqfvdc rrnplessin cfvagdiran eqvgllamht iwlrehnria rslremnphw
      901 ngeklyqear kivgaemqhv tyqhwiphvf dgtaeellgp yrgydpnlda sisnvfataa
      961 lrfghtliqp rlqrlnssfq sipqgplklr daffspwrlv eeggvdplmr gmfgtaaklk
     1021 lpeenlntel teqlfhtaha valdlaamni qrgrdhalpg ylewrricnm shvetfedla
     1081 geihsarvrq klrelyghpg nidvwvggvl edqlpnakvg plfkcllleq frrtrngdrf
     1141 wyesptvfkp dqfaqikqts larilcdngd nidriqpnvf vlpegdnkfv scdeipyvdl
     1201 rvwsdccdgc eeqgntisrf rrsaakylpa qnnfvskdig rhdqteriky leitiqeteq
     1261 eckrvkdlae slsdriedlk slledvkklq
//
LOCUS       XP_034195596            1290 aa            linear   INV 03-SEP-2025
DEFINITION  peroxidasin [Osmia lignaria lignaria].
ACCESSION   XP_034195596
VERSION     XP_034195596.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339705.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1290
                     /product="peroxidasin"
                     /calculated_mol_wt=146097
     Region          <56..>203
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          58..81
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          82..105
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          106..129
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          130..153
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          154..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          236..326
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          253..257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          266..270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          289..293
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          303..308
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          331..417
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          347..351
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          360..364
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          382..387
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          397..402
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          424..497
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          755..1194
                     /region_name="peroxidasin_like"
                     /note="Animal heme peroxidase domain of peroxidasin and
                     related proteins; cd09826"
                     /db_xref="CDD:188658"
     Site            order(794,1063,1066)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:188658"
     Site            order(798,802,804)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:188658"
     Site            order(868,871,958,961..962,965,968,993,1045,1052)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:188658"
     Site            order(922,925..926,930,957,961,963,1119,1123,1127..1128,
                     1131..1132,1135)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:188658"
     CDS             1..1290
                     /gene="Pxn"
                     /coded_by="XM_034339705.2:2015..5887"
                     /db_xref="GeneID:117611656"
ORIGIN      
        1 mrlqlivvsl ilvilrvswa vqsqngrtec phkcmcfgst vrcmfqklnr vprvptnttv
       61 ldlrfnniae lrpgsfynlp elhtlllndn rirhlpprif egapklrily lyknrleris
      121 pgafaglpkl eqlylhynhl reikkgtfnd lpslerlflh snmlhhipad afhnvgpmtr
      181 lrldsnalvc dcnlvwllhr lqskssemaa icqapvemkg rslttmspdd fhctkprimn
      241 gpedvevrlg gtitfacevi gdpapeikwm rdsnevspdg nryviqgdgt liisdaterd
      301 ngeyecvars emgytksrka raiitvspsl rftqlpesqt vqvgtdvyfk crvegqptpe
      361 iqwwrngqtl nvggrisied dgnllriiav kesdsaryvc qarnsngeae tsadlrvvdt
      421 syspprmtye phdmeaepga iievpcrveg vpkpviqwkk dgtavegerv risrggslyl
      481 ynvtaadtgr yecsamnqyg rataqalvrv rqpeatdvlv irafkdatee idrainntli
      541 slftgdssrv npfrlarfpd avgraaarpa evfertlvni rrminagisa nttnefryeq
      601 vltaeqvrei erlsgctghr hkqnctnmcy htkyrsidgs cnnlrhptwg ssytgfrrvl
      661 qpiyengfss pvgwekgrry ygypkpaarl isttlvathn vtsderithm vmqwgqfldh
      721 dldhslpsvs seswdgidck kscdnaapcf pmdvppgdpr vtnrrcidfi rtsavcgsga
      781 tsllwgsftp reqlnqltsy ldasqvygyd dalakdlrdf ttdhgllreg ptipghkpll
      841 pyangqfvdc rrnplessin cfvagdiran eqvgllamht iwlrehnria rslremnphw
      901 ngeklyqear kivgaemqhv tyqhwiphvf dgtaeellgp yrgydpnlda sisnvfataa
      961 lrfghtliqp rlqrlnssfq sipqgplklr daffspwrlv eeggvdplmr gmfgtaaklk
     1021 lpeenlntel teqlfhtaha valdlaamni qrgrdhalpg ylewrricnm shvetfedla
     1081 geihsarvrq klrelyghpg nidvwvggvl edqlpnakvg plfkcllleq frrtrngdrf
     1141 wyesptvfkp dqfaqikqts larilcdngd nidriqpnvf vlpegdnkfv scdeipyvdl
     1201 rvwsdccdgc eeqgntisrf rrsaakylpa qnnfvskdig rhdqteriky leitiqeteq
     1261 eckrvkdlae slsdriedlk slledvkklq
//
LOCUS       XP_034195604            1290 aa            linear   INV 03-SEP-2025
DEFINITION  peroxidasin [Osmia lignaria lignaria].
ACCESSION   XP_034195604
VERSION     XP_034195604.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339713.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1290
                     /product="peroxidasin"
                     /calculated_mol_wt=146097
     Region          <56..>203
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          58..81
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          82..105
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          106..129
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          130..153
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          154..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          236..326
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          253..257
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          266..270
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          289..293
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          303..308
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..322
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          331..417
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          347..351
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          360..364
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          382..387
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          397..402
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          424..497
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          755..1194
                     /region_name="peroxidasin_like"
                     /note="Animal heme peroxidase domain of peroxidasin and
                     related proteins; cd09826"
                     /db_xref="CDD:188658"
     Site            order(794,1063,1066)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:188658"
     Site            order(798,802,804)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:188658"
     Site            order(868,871,958,961..962,965,968,993,1045,1052)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:188658"
     Site            order(922,925..926,930,957,961,963,1119,1123,1127..1128,
                     1131..1132,1135)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:188658"
     CDS             1..1290
                     /gene="Pxn"
                     /coded_by="XM_034339713.2:2094..5966"
                     /db_xref="GeneID:117611656"
ORIGIN      
        1 mrlqlivvsl ilvilrvswa vqsqngrtec phkcmcfgst vrcmfqklnr vprvptnttv
       61 ldlrfnniae lrpgsfynlp elhtlllndn rirhlpprif egapklrily lyknrleris
      121 pgafaglpkl eqlylhynhl reikkgtfnd lpslerlflh snmlhhipad afhnvgpmtr
      181 lrldsnalvc dcnlvwllhr lqskssemaa icqapvemkg rslttmspdd fhctkprimn
      241 gpedvevrlg gtitfacevi gdpapeikwm rdsnevspdg nryviqgdgt liisdaterd
      301 ngeyecvars emgytksrka raiitvspsl rftqlpesqt vqvgtdvyfk crvegqptpe
      361 iqwwrngqtl nvggrisied dgnllriiav kesdsaryvc qarnsngeae tsadlrvvdt
      421 syspprmtye phdmeaepga iievpcrveg vpkpviqwkk dgtavegerv risrggslyl
      481 ynvtaadtgr yecsamnqyg rataqalvrv rqpeatdvlv irafkdatee idrainntli
      541 slftgdssrv npfrlarfpd avgraaarpa evfertlvni rrminagisa nttnefryeq
      601 vltaeqvrei erlsgctghr hkqnctnmcy htkyrsidgs cnnlrhptwg ssytgfrrvl
      661 qpiyengfss pvgwekgrry ygypkpaarl isttlvathn vtsderithm vmqwgqfldh
      721 dldhslpsvs seswdgidck kscdnaapcf pmdvppgdpr vtnrrcidfi rtsavcgsga
      781 tsllwgsftp reqlnqltsy ldasqvygyd dalakdlrdf ttdhgllreg ptipghkpll
      841 pyangqfvdc rrnplessin cfvagdiran eqvgllamht iwlrehnria rslremnphw
      901 ngeklyqear kivgaemqhv tyqhwiphvf dgtaeellgp yrgydpnlda sisnvfataa
      961 lrfghtliqp rlqrlnssfq sipqgplklr daffspwrlv eeggvdplmr gmfgtaaklk
     1021 lpeenlntel teqlfhtaha valdlaamni qrgrdhalpg ylewrricnm shvetfedla
     1081 geihsarvrq klrelyghpg nidvwvggvl edqlpnakvg plfkcllleq frrtrngdrf
     1141 wyesptvfkp dqfaqikqts larilcdngd nidriqpnvf vlpegdnkfv scdeipyvdl
     1201 rvwsdccdgc eeqgntisrf rrsaakylpa qnnfvskdig rhdqteriky leitiqeteq
     1261 eckrvkdlae slsdriedlk slledvkklq
//
LOCUS       XP_034195617             885 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611673 [Osmia lignaria lignaria].
ACCESSION   XP_034195617
VERSION     XP_034195617.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339726.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..885
                     /product="uncharacterized protein LOC117611673"
                     /calculated_mol_wt=99643
     Region          2..75
                     /region_name="RRM1_RBM12_like"
                     /note="RNA recognition motif 1 (RRM1) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12510"
                     /db_xref="CDD:409932"
     Region          449..520
                     /region_name="RRM_hnRNPH_ESRPs_RBM12_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein (hnRNP) H protein family,
                     epithelial splicing regulatory proteins (ESRPs),
                     Drosophila RNA-binding protein Fusilli, RNA-binding
                     protein 12 (RBM12) and similar proteins; cd12254"
                     /db_xref="CDD:409699"
     Region          490..>885
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          652..713
                     /region_name="RRM_hnRNPH_ESRPs_RBM12_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein (hnRNP) H protein family,
                     epithelial splicing regulatory proteins (ESRPs),
                     Drosophila RNA-binding protein Fusilli, RNA-binding
                     protein 12 (RBM12) and similar proteins; cd12254"
                     /db_xref="CDD:409699"
     Region          809..883
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..885
                     /gene="LOC117611673"
                     /coded_by="XM_034339726.2:437..3094"
                     /db_xref="GeneID:117611673"
ORIGIN      
        1 msviirlqnl awsanaldir qffrglsipe ggvhivggel gdafiafstd edarqammhd
       61 ggkikemkik lllssrtemq kvieaarqqt lslqsfmqst pvavppvvqk pgsptdrrek
      121 ekdndsessk drkdrrdrsr srdrsrsrdr drdrdrrdrd rgrdrrrrdr srsrdrerdr
      181 rdrrrrrdrs rsrdrtrsrd rdakrnstge rrkdatdddn ndvvcvgqfh kdkpvpgakk
      241 plengiwevp pqtqmqaaml apgilgagva alsqdgeqrs mmisspvmgh psllqqsqfp
      301 inginsvglm qshvqtlght vgmnslsqni gnsslpnlts gvaslnrske pwnqneqsrm
      361 dqvrfpgrsg qfgtdiygsn gsinyrpgir pnnpflskvq elegrrnpha fqanssqfnf
      421 dndrqssrst tsnsrfssds ksssgsghcv evrnmplsat yndvrhafqg iyirkdglkl
      481 indnhgnrvg iayvkfskae gkelalntpr yvrgsevevl hleeslfdka vdsyspeker
      541 drgedgvedv rtstcilltd lpsftkemdi aklfhdwkin dlfitsskes gstqymayvq
      601 fariedakas vstslkigsk pvtataitee kfaqakrehe qsslnqtass dcvimrglpf
      661 qtidrdildf fsdigivphr ihmllnqsgk pagecfcefd ttdealrata knglpfgknv
      721 ptielvprak mletlgmvdp qvmetqqqhf pplqeqrprf sgpmnhlprf gnsafgprcp
      781 pglmgiprhl lgrhpgsmdy vegfgkpgcv lslenvpfka dineiieffg dfdvkrenvi
      841 rrynergmpt gdarvafasp seaqralrel khckmrdrti ymkla
//
LOCUS       XP_034195626             885 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611673 [Osmia lignaria lignaria].
ACCESSION   XP_034195626
VERSION     XP_034195626.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339735.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..885
                     /product="uncharacterized protein LOC117611673"
                     /calculated_mol_wt=99643
     Region          2..75
                     /region_name="RRM1_RBM12_like"
                     /note="RNA recognition motif 1 (RRM1) found in RNA-binding
                     protein RBM12, RBM12B and similar proteins; cd12510"
                     /db_xref="CDD:409932"
     Region          449..520
                     /region_name="RRM_hnRNPH_ESRPs_RBM12_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein (hnRNP) H protein family,
                     epithelial splicing regulatory proteins (ESRPs),
                     Drosophila RNA-binding protein Fusilli, RNA-binding
                     protein 12 (RBM12) and similar proteins; cd12254"
                     /db_xref="CDD:409699"
     Region          490..>885
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          652..713
                     /region_name="RRM_hnRNPH_ESRPs_RBM12_like"
                     /note="RNA recognition motif (RRM) found in heterogeneous
                     nuclear ribonucleoprotein (hnRNP) H protein family,
                     epithelial splicing regulatory proteins (ESRPs),
                     Drosophila RNA-binding protein Fusilli, RNA-binding
                     protein 12 (RBM12) and similar proteins; cd12254"
                     /db_xref="CDD:409699"
     Region          809..883
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..885
                     /gene="LOC117611673"
                     /coded_by="XM_034339735.2:427..3084"
                     /db_xref="GeneID:117611673"
ORIGIN      
        1 msviirlqnl awsanaldir qffrglsipe ggvhivggel gdafiafstd edarqammhd
       61 ggkikemkik lllssrtemq kvieaarqqt lslqsfmqst pvavppvvqk pgsptdrrek
      121 ekdndsessk drkdrrdrsr srdrsrsrdr drdrdrrdrd rgrdrrrrdr srsrdrerdr
      181 rdrrrrrdrs rsrdrtrsrd rdakrnstge rrkdatdddn ndvvcvgqfh kdkpvpgakk
      241 plengiwevp pqtqmqaaml apgilgagva alsqdgeqrs mmisspvmgh psllqqsqfp
      301 inginsvglm qshvqtlght vgmnslsqni gnsslpnlts gvaslnrske pwnqneqsrm
      361 dqvrfpgrsg qfgtdiygsn gsinyrpgir pnnpflskvq elegrrnpha fqanssqfnf
      421 dndrqssrst tsnsrfssds ksssgsghcv evrnmplsat yndvrhafqg iyirkdglkl
      481 indnhgnrvg iayvkfskae gkelalntpr yvrgsevevl hleeslfdka vdsyspeker
      541 drgedgvedv rtstcilltd lpsftkemdi aklfhdwkin dlfitsskes gstqymayvq
      601 fariedakas vstslkigsk pvtataitee kfaqakrehe qsslnqtass dcvimrglpf
      661 qtidrdildf fsdigivphr ihmllnqsgk pagecfcefd ttdealrata knglpfgknv
      721 ptielvprak mletlgmvdp qvmetqqqhf pplqeqrprf sgpmnhlprf gnsafgprcp
      781 pglmgiprhl lgrhpgsmdy vegfgkpgcv lslenvpfka dineiieffg dfdvkrenvi
      841 rrynergmpt gdarvafasp seaqralrel khckmrdrti ymkla
//
LOCUS       XP_034195681             636 aa            linear   INV 03-SEP-2025
DEFINITION  protein SCAI [Osmia lignaria lignaria].
ACCESSION   XP_034195681
VERSION     XP_034195681.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339790.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="protein SCAI"
                     /calculated_mol_wt=72483
     Region          89..588
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..636
                     /gene="LOC117611677"
                     /coded_by="XM_034339790.2:2276..4186"
                     /db_xref="GeneID:117611677"
ORIGIN      
        1 mvhggvedth gappdagwas idwvgaafvg vvvrvqeggg igrrhhagpp rpssegpssq
       61 wprsrftekm vmmagmddhe rkvvlefchl lekskqlfng lrdlpqyghk qwqgyfgrtf
      121 diytklwkfq qqhrtildtk yglkrwqige iaskigqlyy hyylrtsets ylheaysfya
      181 airgrayysr aakedrsdlm vkklryyarf ivvclllnrm klvrelvqel daqiadytst
      241 yepddqlewn lvldeikafv kaesavgvvh sdsnpvvlth rlgaqtsppv ertppmclsl
      301 qeilivgnca dqvkfselsm dmfrmlqtle reprddpnhl hdaspagrmp frpgpypgae
      361 ngaprrdnph kyllykptys qvqvflasgf kelpangall lylsadgcfs tvkhpeemgy
      421 dlggvstcsk rdpehgkrpt ggkephclyp gdlypftrkp lfvvvdsdns fvfqqipryf
      481 gqplmvlmsp qdtpptlrdv rhggslftlf lhaplaafcl icnigslavh hwercqryie
      541 rflieacqlv trsrcetsvl qffgddflrl llvryvfcdv vlnlhrsfrg rqqrprchpp
      601 lpdaevlehp tlhhlvldla acldcrdhfp dsnela
//
LOCUS       XP_034195636             636 aa            linear   INV 03-SEP-2025
DEFINITION  protein SCAI [Osmia lignaria lignaria].
ACCESSION   XP_034195636
VERSION     XP_034195636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="protein SCAI"
                     /calculated_mol_wt=72483
     Region          89..588
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..636
                     /gene="LOC117611677"
                     /coded_by="XM_034339745.2:2967..4877"
                     /db_xref="GeneID:117611677"
ORIGIN      
        1 mvhggvedth gappdagwas idwvgaafvg vvvrvqeggg igrrhhagpp rpssegpssq
       61 wprsrftekm vmmagmddhe rkvvlefchl lekskqlfng lrdlpqyghk qwqgyfgrtf
      121 diytklwkfq qqhrtildtk yglkrwqige iaskigqlyy hyylrtsets ylheaysfya
      181 airgrayysr aakedrsdlm vkklryyarf ivvclllnrm klvrelvqel daqiadytst
      241 yepddqlewn lvldeikafv kaesavgvvh sdsnpvvlth rlgaqtsppv ertppmclsl
      301 qeilivgnca dqvkfselsm dmfrmlqtle reprddpnhl hdaspagrmp frpgpypgae
      361 ngaprrdnph kyllykptys qvqvflasgf kelpangall lylsadgcfs tvkhpeemgy
      421 dlggvstcsk rdpehgkrpt ggkephclyp gdlypftrkp lfvvvdsdns fvfqqipryf
      481 gqplmvlmsp qdtpptlrdv rhggslftlf lhaplaafcl icnigslavh hwercqryie
      541 rflieacqlv trsrcetsvl qffgddflrl llvryvfcdv vlnlhrsfrg rqqrprchpp
      601 lpdaevlehp tlhhlvldla acldcrdhfp dsnela
//
LOCUS       XP_034195661             636 aa            linear   INV 03-SEP-2025
DEFINITION  protein SCAI [Osmia lignaria lignaria].
ACCESSION   XP_034195661
VERSION     XP_034195661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="protein SCAI"
                     /calculated_mol_wt=72483
     Region          89..588
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..636
                     /gene="LOC117611677"
                     /coded_by="XM_034339770.2:2892..4802"
                     /db_xref="GeneID:117611677"
ORIGIN      
        1 mvhggvedth gappdagwas idwvgaafvg vvvrvqeggg igrrhhagpp rpssegpssq
       61 wprsrftekm vmmagmddhe rkvvlefchl lekskqlfng lrdlpqyghk qwqgyfgrtf
      121 diytklwkfq qqhrtildtk yglkrwqige iaskigqlyy hyylrtsets ylheaysfya
      181 airgrayysr aakedrsdlm vkklryyarf ivvclllnrm klvrelvqel daqiadytst
      241 yepddqlewn lvldeikafv kaesavgvvh sdsnpvvlth rlgaqtsppv ertppmclsl
      301 qeilivgnca dqvkfselsm dmfrmlqtle reprddpnhl hdaspagrmp frpgpypgae
      361 ngaprrdnph kyllykptys qvqvflasgf kelpangall lylsadgcfs tvkhpeemgy
      421 dlggvstcsk rdpehgkrpt ggkephclyp gdlypftrkp lfvvvdsdns fvfqqipryf
      481 gqplmvlmsp qdtpptlrdv rhggslftlf lhaplaafcl icnigslavh hwercqryie
      541 rflieacqlv trsrcetsvl qffgddflrl llvryvfcdv vlnlhrsfrg rqqrprchpp
      601 lpdaevlehp tlhhlvldla acldcrdhfp dsnela
//
LOCUS       XP_034195670             636 aa            linear   INV 03-SEP-2025
DEFINITION  protein SCAI [Osmia lignaria lignaria].
ACCESSION   XP_034195670
VERSION     XP_034195670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339779.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="protein SCAI"
                     /calculated_mol_wt=72483
     Region          89..588
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..636
                     /gene="LOC117611677"
                     /coded_by="XM_034339779.2:2802..4712"
                     /db_xref="GeneID:117611677"
ORIGIN      
        1 mvhggvedth gappdagwas idwvgaafvg vvvrvqeggg igrrhhagpp rpssegpssq
       61 wprsrftekm vmmagmddhe rkvvlefchl lekskqlfng lrdlpqyghk qwqgyfgrtf
      121 diytklwkfq qqhrtildtk yglkrwqige iaskigqlyy hyylrtsets ylheaysfya
      181 airgrayysr aakedrsdlm vkklryyarf ivvclllnrm klvrelvqel daqiadytst
      241 yepddqlewn lvldeikafv kaesavgvvh sdsnpvvlth rlgaqtsppv ertppmclsl
      301 qeilivgnca dqvkfselsm dmfrmlqtle reprddpnhl hdaspagrmp frpgpypgae
      361 ngaprrdnph kyllykptys qvqvflasgf kelpangall lylsadgcfs tvkhpeemgy
      421 dlggvstcsk rdpehgkrpt ggkephclyp gdlypftrkp lfvvvdsdns fvfqqipryf
      481 gqplmvlmsp qdtpptlrdv rhggslftlf lhaplaafcl icnigslavh hwercqryie
      541 rflieacqlv trsrcetsvl qffgddflrl llvryvfcdv vlnlhrsfrg rqqrprchpp
      601 lpdaevlehp tlhhlvldla acldcrdhfp dsnela
//
LOCUS       XP_034195691             636 aa            linear   INV 03-SEP-2025
DEFINITION  protein SCAI [Osmia lignaria lignaria].
ACCESSION   XP_034195691
VERSION     XP_034195691.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339800.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="protein SCAI"
                     /calculated_mol_wt=72483
     Region          89..588
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..636
                     /gene="LOC117611677"
                     /coded_by="XM_034339800.2:2678..4588"
                     /db_xref="GeneID:117611677"
ORIGIN      
        1 mvhggvedth gappdagwas idwvgaafvg vvvrvqeggg igrrhhagpp rpssegpssq
       61 wprsrftekm vmmagmddhe rkvvlefchl lekskqlfng lrdlpqyghk qwqgyfgrtf
      121 diytklwkfq qqhrtildtk yglkrwqige iaskigqlyy hyylrtsets ylheaysfya
      181 airgrayysr aakedrsdlm vkklryyarf ivvclllnrm klvrelvqel daqiadytst
      241 yepddqlewn lvldeikafv kaesavgvvh sdsnpvvlth rlgaqtsppv ertppmclsl
      301 qeilivgnca dqvkfselsm dmfrmlqtle reprddpnhl hdaspagrmp frpgpypgae
      361 ngaprrdnph kyllykptys qvqvflasgf kelpangall lylsadgcfs tvkhpeemgy
      421 dlggvstcsk rdpehgkrpt ggkephclyp gdlypftrkp lfvvvdsdns fvfqqipryf
      481 gqplmvlmsp qdtpptlrdv rhggslftlf lhaplaafcl icnigslavh hwercqryie
      541 rflieacqlv trsrcetsvl qffgddflrl llvryvfcdv vlnlhrsfrg rqqrprchpp
      601 lpdaevlehp tlhhlvldla acldcrdhfp dsnela
//
LOCUS       XP_034195723             636 aa            linear   INV 03-SEP-2025
DEFINITION  protein SCAI [Osmia lignaria lignaria].
ACCESSION   XP_034195723
VERSION     XP_034195723.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339832.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="protein SCAI"
                     /calculated_mol_wt=72483
     Region          89..588
                     /region_name="SCAI"
                     /note="Protein SCAI; pfam12070"
                     /db_xref="CDD:463455"
     CDS             1..636
                     /gene="LOC117611677"
                     /coded_by="XM_034339832.2:1427..3337"
                     /db_xref="GeneID:117611677"
ORIGIN      
        1 mvhggvedth gappdagwas idwvgaafvg vvvrvqeggg igrrhhagpp rpssegpssq
       61 wprsrftekm vmmagmddhe rkvvlefchl lekskqlfng lrdlpqyghk qwqgyfgrtf
      121 diytklwkfq qqhrtildtk yglkrwqige iaskigqlyy hyylrtsets ylheaysfya
      181 airgrayysr aakedrsdlm vkklryyarf ivvclllnrm klvrelvqel daqiadytst
      241 yepddqlewn lvldeikafv kaesavgvvh sdsnpvvlth rlgaqtsppv ertppmclsl
      301 qeilivgnca dqvkfselsm dmfrmlqtle reprddpnhl hdaspagrmp frpgpypgae
      361 ngaprrdnph kyllykptys qvqvflasgf kelpangall lylsadgcfs tvkhpeemgy
      421 dlggvstcsk rdpehgkrpt ggkephclyp gdlypftrkp lfvvvdsdns fvfqqipryf
      481 gqplmvlmsp qdtpptlrdv rhggslftlf lhaplaafcl icnigslavh hwercqryie
      541 rflieacqlv trsrcetsvl qffgddflrl llvryvfcdv vlnlhrsfrg rqqrprchpp
      601 lpdaevlehp tlhhlvldla acldcrdhfp dsnela
//
LOCUS       XP_034195738             627 aa            linear   INV 03-SEP-2025
DEFINITION  carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase
            protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195738
VERSION     XP_034195738.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339847.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein isoform X1"
                     /calculated_mol_wt=70135
     Region          3..172
                     /region_name="PTB_CAPON-like"
                     /note="Carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein (CAPON) Phosphotyrosine-binding
                     (PTB) domain; cd01270"
                     /db_xref="CDD:269968"
     Site            order(36,123,143)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269968"
     Site            order(112..117,130,154,158)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269968"
     Region          270..>332
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..627
                     /gene="LOC117611706"
                     /coded_by="XM_034339847.2:973..2856"
                     /db_xref="GeneID:117611706"
ORIGIN      
        1 mpskkqynlv hndeydtrip lhseeafhrg ivfhakfigs mevprptsrv eivaamrrir
       61 yefkakgikk kkvtlevsvd glkvtlrkkk kkqqqwmden kiylmhhpiy rifyvshdsh
      121 dlkifsyiar dgssntfkcn vfksskksqa mrvvrtvgqa fevchklsin natedrerek
      181 ererdhenhr dvyedqdeip neqsqpspss vhkdisllgd tedsvaeqtt vpcllrshev
      241 pattastspi rqspsgmvts dcggllvgge ltalkheiql lrerleqqsq qtraavahar
      301 llqdqlaaet aarveaqart hqllmqnkel lehigalvgh lreqerissg hvtsqsqipg
      361 satmqqtttv pdlsnlgqcp rtggqsspwe ldppspycsy ppeslyagnl ntigiqgnst
      421 sadqlqfqtq llerlhnisp yqpqrspynt pspyamgpsl llppssrpsn saqlspnsms
      481 lrvsqpnsfs sspvmthkld sygvnpdiid skstfikplp ctgerntvhl vqeakgkqdr
      541 inphdeippi vldpppqgkr seatakqake nrngmnnkkn pvkqktlati lrtpgpppsr
      601 ttsarlpsrs elmsevqrtt warhttk
//
LOCUS       XP_034195747             627 aa            linear   INV 03-SEP-2025
DEFINITION  carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase
            protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195747
VERSION     XP_034195747.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339856.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein isoform X1"
                     /calculated_mol_wt=70135
     Region          3..172
                     /region_name="PTB_CAPON-like"
                     /note="Carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein (CAPON) Phosphotyrosine-binding
                     (PTB) domain; cd01270"
                     /db_xref="CDD:269968"
     Site            order(36,123,143)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269968"
     Site            order(112..117,130,154,158)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269968"
     Region          270..>332
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..627
                     /gene="LOC117611706"
                     /coded_by="XM_034339856.2:970..2853"
                     /db_xref="GeneID:117611706"
ORIGIN      
        1 mpskkqynlv hndeydtrip lhseeafhrg ivfhakfigs mevprptsrv eivaamrrir
       61 yefkakgikk kkvtlevsvd glkvtlrkkk kkqqqwmden kiylmhhpiy rifyvshdsh
      121 dlkifsyiar dgssntfkcn vfksskksqa mrvvrtvgqa fevchklsin natedrerek
      181 ererdhenhr dvyedqdeip neqsqpspss vhkdisllgd tedsvaeqtt vpcllrshev
      241 pattastspi rqspsgmvts dcggllvgge ltalkheiql lrerleqqsq qtraavahar
      301 llqdqlaaet aarveaqart hqllmqnkel lehigalvgh lreqerissg hvtsqsqipg
      361 satmqqtttv pdlsnlgqcp rtggqsspwe ldppspycsy ppeslyagnl ntigiqgnst
      421 sadqlqfqtq llerlhnisp yqpqrspynt pspyamgpsl llppssrpsn saqlspnsms
      481 lrvsqpnsfs sspvmthkld sygvnpdiid skstfikplp ctgerntvhl vqeakgkqdr
      541 inphdeippi vldpppqgkr seatakqake nrngmnnkkn pvkqktlati lrtpgpppsr
      601 ttsarlpsrs elmsevqrtt warhttk
//
LOCUS       XP_076546032             572 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611706 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546032
VERSION     XP_076546032.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689917.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="uncharacterized protein LOC117611706 isoform X2"
                     /calculated_mol_wt=62905
     Region          <93..117
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          <215..>295
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..572
                     /gene="LOC117611706"
                     /coded_by="XM_076689917.1:1..1719"
                     /db_xref="GeneID:117611706"
ORIGIN      
        1 mlknstkqvn sadasassas gpaspsavet tpkprkklsf kepeifnylk lrkpfgkakp
       61 pplrltrstg svdfnfdenp feeenddlee lesqamrvvr tvgqafevch klsinnated
      121 rerekererd henhrdvyed qdeipneqsq pspssvhkdi sllgdtedsv aeqttvpcll
      181 rshevpatta stspirqsps gmvtsdcggl lvggeltalk heiqllrerl eqqsqqtraa
      241 vaharllqdq laaetaarve aqarthqllm qnkellehig alvghlreqe rissghvtsq
      301 sqipgsatmq qtttvpdlsn lgqcprtggq sspweldpps pycsyppesl yagnlntigi
      361 qgnstsadql qfqtqllerl hnispyqpqr spyntpspya mgpslllpps srpsnsaqls
      421 pnsmslrvsq pnsfssspvm thkldsygvn pdiidskstf ikplpctger ntvhlvqeak
      481 gkqdrinphd eippivldpp pqgkrseata kqakenrngm nnkknpvkqk tlatilrtpg
      541 pppsrttsar lpsrselmse vqrttwarht tk
//
LOCUS       XP_034195772             536 aa            linear   INV 03-SEP-2025
DEFINITION  carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase
            protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195772
VERSION     XP_034195772.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339881.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..536
                     /product="carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein isoform X3"
                     /calculated_mol_wt=59925
     Region          3..172
                     /region_name="PTB_CAPON-like"
                     /note="Carboxyl-terminal PDZ ligand of neuronal nitric
                     oxide synthase protein (CAPON) Phosphotyrosine-binding
                     (PTB) domain; cd01270"
                     /db_xref="CDD:269968"
     Site            order(36,123,143)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269968"
     Site            order(112..117,130,154,158)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269968"
     Region          270..>332
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..536
                     /gene="LOC117611706"
                     /coded_by="XM_034339881.2:972..2582"
                     /db_xref="GeneID:117611706"
ORIGIN      
        1 mpskkqynlv hndeydtrip lhseeafhrg ivfhakfigs mevprptsrv eivaamrrir
       61 yefkakgikk kkvtlevsvd glkvtlrkkk kkqqqwmden kiylmhhpiy rifyvshdsh
      121 dlkifsyiar dgssntfkcn vfksskksqa mrvvrtvgqa fevchklsin natedrerek
      181 ererdhenhr dvyedqdeip neqsqpspss vhkdisllgd tedsvaeqtt vpcllrshev
      241 pattastspi rqspsgmvts dcggllvgge ltalkheiql lrerleqqsq qtraavahar
      301 llqdqlaaet aarveaqart hqllmqnkel lehigalvgh lreqerissg hvtsqsqipg
      361 satmqqtttv pdlsnlgqcp rtggqsspwe ldppspycsy ppeslyagnl ntigiqgnst
      421 sadqlqfqtq llerlhnisp yqpqrspynt pspyamgpsl llppssrpsn saqlspnsms
      481 lrvsqpnsfs sspvmthkld sygvnpdiid skstfikplp ctgerntvhl vqeakg
//
LOCUS       XP_076546033             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546033
VERSION     XP_076546033.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689918.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689918.1:1484..2662"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546034             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546034
VERSION     XP_076546034.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689919.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689919.1:1888..3066"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546035             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546035
VERSION     XP_076546035.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689920.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689920.1:1745..2923"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_034195870             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195870
VERSION     XP_034195870.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_034339979.2:1182..2360"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546036             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546036
VERSION     XP_076546036.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689921.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689921.1:1642..2820"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_034195877             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195877
VERSION     XP_034195877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_034339986.2:1529..2707"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546037             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546037
VERSION     XP_076546037.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689922.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689922.1:1829..3007"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546038             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546038
VERSION     XP_076546038.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689923.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689923.1:1497..2675"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546039             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546039
VERSION     XP_076546039.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689924.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689924.1:1268..2446"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546040             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546040
VERSION     XP_076546040.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689925.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689925.1:1240..2418"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_076546041             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611727 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546041
VERSION     XP_076546041.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689926.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117611727 isoform X1"
                     /calculated_mol_wt=42921
     CDS             1..392
                     /gene="LOC117611727"
                     /coded_by="XM_076689926.1:979..2157"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mpgevraqpl nsttysgssm mqpdeeiqya prsrpvsfyd nfkdvpmvtp cvtsalsadn
       61 lnqvrdnpsm atannnnnnn nnrvsnavsv gnvskiqstk vtgavstgni gkiyrsdkek
      121 elgrapsman clsttvpvnl aasqiagrht ptrnslrhsr mivlhrsghr pqkflpplvy
      181 hwrlgrclaa lqtilgvavt slslwlllwa phlpvadnpy wsgmplllsg sfgvcllccf
      241 kkeypgmspg fclastkvis vslailatvt csiacvfsai hlarlmrlec nparvlnatc
      301 vcrpredasn stetavrymd lncpevesil tillifsstc nalgalvagw ytylhwstrh
      361 krpkymqvrt sptsgnnfls srpiynpnln er
//
LOCUS       XP_034195907             346 aa            linear   INV 03-SEP-2025
DEFINITION  sarcospan isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195907
VERSION     XP_034195907.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340016.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="sarcospan isoform X2"
                     /calculated_mol_wt=37714
     CDS             1..346
                     /gene="LOC117611727"
                     /coded_by="XM_034340016.2:151..1191"
                     /db_xref="GeneID:117611727"
ORIGIN      
        1 mvtpcvtsal sadnlnqvrd npsmatannn nnnnnnrvsn avsvgnvski qstkvtgavs
       61 tgnigkiyrs dkekelgrap smanclsttv pvnlaasqia grhtptrnsl rhsrmivlhr
      121 sghrpqkflp plvyhwrlgr claalqtilg vavtslslwl llwaphlpva dnpywsgmpl
      181 llsgsfgvcl lccfkkeypg mspgfclast kvisvslail atvtcsiacv fsaihlarlm
      241 rlecnparvl natcvcrpre dasnstetav rymdlncpev esiltillif sstcnalgal
      301 vagwytylhw strhkrpkym qvrtsptsgn nflssrpiyn pnlner
//
LOCUS       XP_034195922             389 aa            linear   INV 03-SEP-2025
DEFINITION  mevalonate diphosphate decarboxylase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195922
VERSION     XP_034195922.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340031.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="mevalonate diphosphate decarboxylase isoform X1"
                     /calculated_mol_wt=43283
     Region          3..325
                     /region_name="MDD_C"
                     /note="Mevalonate 5-diphosphate decarboxylase C-terminal
                     domain; cl28577"
                     /db_xref="CDD:475042"
     CDS             1..389
                     /gene="Mvd"
                     /coded_by="XM_034340031.2:102..1271"
                     /db_xref="GeneID:117611770"
ORIGIN      
        1 mstvtclapv niavikywgk kneklilpln dsisttidte qlcakttvmi spdfkedciw
       61 lngqeedikn prlqncltei rkraggskql nqwkihicse nnfptaagla ssaagyaclv
      121 ttlaklyeve gdisaiarlg sgsacrstvg gfvrwymgse pngtdsivkq iapathwpem
      181 rililvvnds kkkvssaigm krtmetsell khrvkhvvpd ranrmqqaii ekdfktyaei
      241 tmkdsnqlha lnldtyppni ymndvshaiv elvhsyneav ndvkvaytfd agpnatlyll
      301 ekevpivmgi ldyffppsed vlieykkglp idtvkpsevn dllkkinvpk halgcfkyii
      361 htqigdgpky ledpkdhlln sqgwpinhv
//
LOCUS       XP_034195932             387 aa            linear   INV 03-SEP-2025
DEFINITION  mevalonate diphosphate decarboxylase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195932
VERSION     XP_034195932.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340041.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..387
                     /product="mevalonate diphosphate decarboxylase isoform X2"
                     /calculated_mol_wt=43070
     Region          3..325
                     /region_name="MDD_C"
                     /note="Mevalonate 5-diphosphate decarboxylase C-terminal
                     domain; cl28577"
                     /db_xref="CDD:475042"
     CDS             1..387
                     /gene="Mvd"
                     /coded_by="XM_034340041.2:100..1263"
                     /db_xref="GeneID:117611770"
ORIGIN      
        1 mstvtclapv niavikywgk kneklilpln dsisttidte qlcakttvmi spdfkedciw
       61 lngqeedikn prlqncltei rkraggskql nqwkihicse nnfptaagla ssaagyaclv
      121 ttlaklyeve gdisaiarlg sgsacrstvg gfvrwymgse pngtdsivkq iapathwpem
      181 rililvvnds kkkvssaigm krtmetsell khrvkhvvpd ranrmqqaii ekdfktyaei
      241 tmkdsnqlha lnldtyppni ymndvshaiv elvhsyneav ndvkvaytfd agpnatlyll
      301 ekevpivmgi ldyffppsed vlieykkglp idtvkpsedl lkkinvpkha lgcfkyiiht
      361 qigdgpkyle dpkdhllnsq gwpinhv
//
LOCUS       XP_034195954             330 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-binding protein D-ETS-3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195954
VERSION     XP_034195954.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340063.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="DNA-binding protein D-ETS-3 isoform X1"
                     /calculated_mol_wt=35008
     Region          159..244
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..330
                     /gene="Ets65A"
                     /coded_by="XM_034340063.2:1224..2216"
                     /db_xref="GeneID:117611779"
ORIGIN      
        1 mvnvdgvqia fyrghrrsds rgslvmydsa scsyagalel kgasgagaaa aaagvtaagv
       61 kqenwpyrgl tcgstfqrlk esvdkakqal adrggylsga fspppranps aisptasitd
      121 asssykggws hatspapgqd pyqmfgatss rlassgsgqi qlwqfllell sdssnaacit
      181 wegtngefkl tdpdevarrw gerkskpnmn ydklsralry yydknimtkv hgkryaykfd
      241 fqglaaatqp aaadpaayky qselfmsgyg haklnlmppp aasvsvsvpg glfqsassyw
      301 stspganlya ghhtasghvp phlgtyphya
//
LOCUS       XP_034195963             325 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-binding protein D-ETS-3 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195963
VERSION     XP_034195963.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340072.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..325
                     /product="DNA-binding protein D-ETS-3 isoform X2"
                     /calculated_mol_wt=34343
     Region          154..239
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..325
                     /gene="Ets65A"
                     /coded_by="XM_034340072.2:305..1282"
                     /db_xref="GeneID:117611779"
ORIGIN      
        1 mydsascsya galelkgasg agaaaaaagv taagvkqenw pyrgltcgst fqrlkesvdk
       61 akqaladrgg ylsgafsppp ranpsaispt asitdasssy kggwshatsp apgqgygssl
      121 sksaldthth lrqpdpyqmf gatssrlass gsgqiqlwqf llellsdssn aacitwegtn
      181 gefkltdpde varrwgerks kpnmnydkls ralryyydkn imtkvhgkry aykfdfqgla
      241 aatqpaaadp aaykyqself msgyghakln lmpppaasvs vsvpgglfqs assywstspg
      301 anlyaghhta sghvpphlgt yphya
//
LOCUS       XP_034195944             350 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-binding protein D-ETS-3 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195944
VERSION     XP_034195944.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340053.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="DNA-binding protein D-ETS-3 isoform X3"
                     /calculated_mol_wt=37145
     Region          179..264
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..350
                     /gene="Ets65A"
                     /coded_by="XM_034340053.2:67..1119"
                     /db_xref="GeneID:117611779"
ORIGIN      
        1 mvnvdgvqia fyrghrrsds rgslvmydsa scsyagalel kgasgagaaa aaagvtaagv
       61 kqenwpyrgl tcgstfqrlk esvdkakqal adrggylsga fspppranps aisptasitd
      121 asssykggws hatspapgqg ygsslsksal dththlrqpd pyqmfgatss rlassgsgqi
      181 qlwqfllell sdssnaacit wegtngefkl tdpdevarrw gerkskpnmn ydklsralry
      241 yydknimtkv hgkryaykfd fqglaaatqp aaadpaayky qselfmsgyg haklnlmppp
      301 aasvsvsvpg glfqsassyw stspganlya ghhtasghvp phlgtyphya
//
LOCUS       XP_034195985             275 aa            linear   INV 03-SEP-2025
DEFINITION  negative elongation factor E [Osmia lignaria lignaria].
ACCESSION   XP_034195985
VERSION     XP_034195985.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340094.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="negative elongation factor E"
                     /calculated_mol_wt=31362
     Region          157..231
                     /region_name="RRM_NELFE"
                     /note="RNA recognition motif in negative elongation factor
                     E (NELF-E) and similar proteins; cd12305"
                     /db_xref="CDD:409746"
     CDS             1..275
                     /gene="Nelf-E"
                     /coded_by="XM_034340094.2:119..946"
                     /db_xref="GeneID:117611795"
ORIGIN      
        1 mqnmvylhfp snlteeelml qakynklkrk kkalqdlksp kqeteripqt pkrptearda
       61 revakkliks gvitapktpk rpeqsfkrpr glvrklnste ktvssyqpfs atqmeeeete
      121 tarprvkdly dsfvsaqdte drtgsdtqsp skqetkprag ntifvcgyki sedflkkhfq
      181 tfgniinism eaeknrgfit fdkseaaera isemdgsmvs siqlkvslar rqpiiesind
      241 vtsssmwspi aanysqksvh kdrrdlkvye edlfv
//
LOCUS       XP_034195998             246 aa            linear   INV 03-SEP-2025
DEFINITION  pyrimidodiazepine synthase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195998
VERSION     XP_034195998.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340107.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195998.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..246
                     /product="pyrimidodiazepine synthase isoform X1"
                     /calculated_mol_wt=28437
     Region          2..90
                     /region_name="GST_N_Omega"
                     /note="GST_N family, Class Omega subfamily; GSTs are
                     cytosolic dimeric proteins involved in cellular
                     detoxification by catalyzing the conjugation of
                     glutathione (GSH) with a wide range of endogenous and
                     xenobiotic alkylating agents, including carcinogens;
                     cd03055"
                     /db_xref="CDD:239353"
     Site            order(4..5,25..27,29,32..33,35..37,39..40,51,53)
                     /site_type="other"
                     /note="C-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:239353"
     Site            order(28,30,55,67..69,81..82)
                     /site_type="other"
                     /note="GSH binding site (G-site) [chemical binding]"
                     /db_xref="CDD:239353"
     Site            28
                     /site_type="active"
                     /note="active site cysteine [active]"
                     /db_xref="CDD:239353"
     Site            order(67,80..81,83..84,87)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:239353"
     Region          106..229
                     /region_name="GST_C_Omega"
                     /note="C-terminal, alpha helical domain of Class Omega
                     Glutathione S-transferases; cd03184"
                     /db_xref="CDD:198293"
     Site            order(110..111,114..115,118,146)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:198293"
     Site            order(117,120..121,124..125,174,177,222)
                     /site_type="other"
                     /note="substrate binding pocket (H-site) [chemical
                     binding]"
                     /db_xref="CDD:198293"
     Site            order(166,169..170,173,176..177,211,215,225,229)
                     /site_type="other"
                     /note="N-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:198293"
     CDS             1..246
                     /gene="LOC117611799"
                     /coded_by="XM_034340107.2:178..918"
                     /db_xref="GeneID:117611799"
ORIGIN      
        1 mssthlssgs vappvvpgki rlysmrfcpy aqrihlvlda khipydvvyv nlthkpewli
       61 eksplgkvpc iefqsgetly esliiadcld esypqnklyp ddpvaraknk llisrfntvi
      121 nimyklyqnt sadrdvfnea ltelglfere ltlrktlffh gnspgmvdfm iwpwfersdi
      181 ikflrgdqft iprdrfkrlm ewksamkenp avqcsyldte vhakyiqsrq agtpqydfvv
      241 qatklm
//
LOCUS       XP_076546042             222 aa            linear   INV 03-SEP-2025
DEFINITION  pyrimidodiazepine synthase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546042
VERSION     XP_076546042.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689927.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="pyrimidodiazepine synthase isoform X2"
                     /calculated_mol_wt=25826
     Region          2..>65
                     /region_name="Thioredoxin_like"
                     /note="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold; cl00388"
                     /db_xref="CDD:469754"
     Region          82..205
                     /region_name="GST_C_Omega"
                     /note="C-terminal, alpha helical domain of Class Omega
                     Glutathione S-transferases; cd03184"
                     /db_xref="CDD:198293"
     Site            order(86..87,90..91,94,122)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:198293"
     Site            order(93,96..97,100..101,150,153,198)
                     /site_type="other"
                     /note="substrate binding pocket (H-site) [chemical
                     binding]"
                     /db_xref="CDD:198293"
     Site            order(142,145..146,149,152..153,187,191,201,205)
                     /site_type="other"
                     /note="N-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:198293"
     CDS             1..222
                     /gene="LOC117611799"
                     /coded_by="XM_076689927.1:185..853"
                     /db_xref="GeneID:117611799"
ORIGIN      
        1 mssthlssgs vappvvpgki rlysmrfcpy aqrihlvlda khipydvvyv nlthkpewli
       61 ekspldesyp qnklypddpv araknkllis rfntvinimy klyqntsadr dvfnealtel
      121 glfereltlr ktlffhgnsp gmvdfmiwpw fersdiikfl rgdqftiprd rfkrlmewks
      181 amkenpavqc syldtevhak yiqsrqagtp qydfvvqatk lm
//
LOCUS       XP_034196019             228 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196019
VERSION     XP_034196019.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340128.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="cleavage and polyadenylation specificity factor
                     subunit 5"
                     /calculated_mol_wt=25997
     Region          40..222
                     /region_name="NUDIX_Cfim25_Nudt21"
                     /note="25 kDa subunit of cleavage factor Im; cd18871"
                     /db_xref="CDD:467583"
     CDS             1..228
                     /gene="Cpsf5"
                     /coded_by="XM_034340128.2:140..826"
                     /db_xref="GeneID:117611810"
ORIGIN      
        1 mamattqvkg sgwprrasns sfegkvvqnq svtinrtvnl ypltnytfgt keplfekdps
       61 vparfqrmrd efdkigmrrs vegvllvheh glphvlllql gttffklpgg elnagedeve
      121 glkrlltetf grqdgvkqew viedtignww rpnfeppqyp yvpphitkpk ehkrlflvql
      181 qekalfavpk nyklvaaplf elydnsqgyg piisslpqsl crfnfiym
//
LOCUS       XP_034196033             142 aa            linear   INV 03-SEP-2025
DEFINITION  acyl-coenzyme A thioesterase 13 [Osmia lignaria lignaria].
ACCESSION   XP_034196033
VERSION     XP_034196033.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..142
                     /product="acyl-coenzyme A thioesterase 13"
                     /calculated_mol_wt=14933
     Region          27..137
                     /region_name="PaaI_thioesterase"
                     /note="PaaI_thioesterase is a tetrameric acyl-CoA
                     thioesterase with a hot dog fold and one of several
                     proteins responsible for phenylacetic acid (PA)
                     degradation in bacteria. Although orthologs of PaaI exist
                     in archaea and eukaryotes, their function has not...;
                     cd03443"
                     /db_xref="CDD:239527"
     Site            order(54,82,89..92)
                     /site_type="other"
                     /note="CoenzymeA binding site [chemical binding]"
                     /db_xref="CDD:239527"
     Site            order(55,57,60,83..87,89)
                     /site_type="other"
                     /note="subunit interaction site [polypeptide binding]"
                     /db_xref="CDD:239527"
     Site            order(56,64..65,68..69,82)
                     /site_type="active"
                     /note="PHB binding site [active]"
                     /db_xref="CDD:239527"
     CDS             1..142
                     /gene="LOC117611815"
                     /coded_by="XM_034340142.2:216..644"
                     /db_xref="GeneID:117611815"
ORIGIN      
        1 mcktklikav venlisktnf gqcmkkikii sagsgnckae lvisdehlnv ggtlhggfts
       61 tlidcistya vmtqgtgvpg vsvdlhvtfm kaafpgelit idaktvragk slaflavdvt
      121 knngidmvah grhtkfllqg kq
//
LOCUS       XP_034196048              98 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611817 [Osmia lignaria lignaria].
ACCESSION   XP_034196048
VERSION     XP_034196048.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340157.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..98
                     /product="uncharacterized protein LOC117611817"
                     /calculated_mol_wt=11428
     Region          7..88
                     /region_name="CCDC-167"
                     /note="Coiled-coil domain-containing protein 167;
                     pfam15188"
                     /db_xref="CDD:464553"
     CDS             1..98
                     /gene="LOC117611817"
                     /coded_by="XM_034340157.2:404..700"
                     /db_xref="GeneID:117611817"
ORIGIN      
        1 marnetimtk iqkvedtlkd smykvdvlek klrtklltie sreqletkle eikgvlkkne
       61 eklkhlrken tksfmvagsl vficfllygl ylmiygki
//
LOCUS       XP_034183281             950 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 [Osmia lignaria lignaria].
ACCESSION   XP_034183281
VERSION     XP_034183281.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327390.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183281.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..950
                     /product="discoidin domain-containing receptor 2"
                     /calculated_mol_wt=107396
     Region          66..217
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(106,137,145)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          648..937
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(660..663,666,668,692,694,724,740..743,796,800..801,
                     803,813..814)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..950
                     /gene="Ddr"
                     /coded_by="XM_034327390.2:640..3492"
                     /db_xref="GeneID:117605712"
ORIGIN      
        1 mwsgsstvsd eeerlvgvpm aqvtsnmpvl eprpdpaplr alligvflll ilprchcfdl
       61 gqctaalgme ngeipdedis assmydpslg pkharlrqdk gggawcpknm vtkegkeyle
      121 vnlhnprilt stktqgrfgn ghgveyteey fveywrpgfn kwvrwrnrrg mellagnnnp
      181 ysekeqvfdp aivatkirfi pytshmrmvc irvelygcpw teglvsysmp qgikrgsevd
      241 lsdrtydgke eggylsgglg qlvdgqkgpd nfrldvsgng kgyewvgwrn dtpsmlgrpv
      301 eitfefdysr nftaihlhmn nfftkdvqvf syakvylgag anqfngepvh fsyipdlvle
      361 qardvtiklh sragrflklq lyfaarwiml sevifesvis ewnntedeea rnksaivsat
      421 gspyqnnegp lqrdevkatf nkeenndnal pdkskepesk qfvglvigil ttvivmllaa
      481 imfifyrnrr lkaalapstf ydqhgdlkvs vqeegedkgp icpplpaqyh paayttttpq
      541 lhktitdysg itevqpvipl llntainltr pippvqeyps npppippppe kyyasteick
      601 nslpplppsp tpstpppmsa kasssmtsys pedmlteeed evpecildfp reklnivenl
      661 gcgyfgdvhv ceidrfpgyd evfrntssdl vivkslrpgs sdalriefqq eakrvarlad
      721 rnvarllgas leddpmcivl engeygdlnq ylqshiaets tvqtaktlsf gtlvymatqi
      781 asgmkhleem dfvhrdlatr nclvsrgytv kvsdlgsgrn ayaadyfrve grpplpirwm
      841 awesmlmgrh ttksdvwsfa vtlweiltfa reqpfeelpd hrivenatyf yqeddrriil
      901 plpkncpkdi yelmrecwhr ndvdrpnfre ihmflqrknl gykhadtndt
//
LOCUS       XP_034183368             950 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 [Osmia lignaria lignaria].
ACCESSION   XP_034183368
VERSION     XP_034183368.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327477.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183368.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..950
                     /product="discoidin domain-containing receptor 2"
                     /calculated_mol_wt=107396
     Region          66..217
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(106,137,145)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          648..937
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(660..663,666,668,692,694,724,740..743,796,800..801,
                     803,813..814)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..950
                     /gene="Ddr"
                     /coded_by="XM_034327477.2:506..3358"
                     /db_xref="GeneID:117605712"
ORIGIN      
        1 mwsgsstvsd eeerlvgvpm aqvtsnmpvl eprpdpaplr alligvflll ilprchcfdl
       61 gqctaalgme ngeipdedis assmydpslg pkharlrqdk gggawcpknm vtkegkeyle
      121 vnlhnprilt stktqgrfgn ghgveyteey fveywrpgfn kwvrwrnrrg mellagnnnp
      181 ysekeqvfdp aivatkirfi pytshmrmvc irvelygcpw teglvsysmp qgikrgsevd
      241 lsdrtydgke eggylsgglg qlvdgqkgpd nfrldvsgng kgyewvgwrn dtpsmlgrpv
      301 eitfefdysr nftaihlhmn nfftkdvqvf syakvylgag anqfngepvh fsyipdlvle
      361 qardvtiklh sragrflklq lyfaarwiml sevifesvis ewnntedeea rnksaivsat
      421 gspyqnnegp lqrdevkatf nkeenndnal pdkskepesk qfvglvigil ttvivmllaa
      481 imfifyrnrr lkaalapstf ydqhgdlkvs vqeegedkgp icpplpaqyh paayttttpq
      541 lhktitdysg itevqpvipl llntainltr pippvqeyps npppippppe kyyasteick
      601 nslpplppsp tpstpppmsa kasssmtsys pedmlteeed evpecildfp reklnivenl
      661 gcgyfgdvhv ceidrfpgyd evfrntssdl vivkslrpgs sdalriefqq eakrvarlad
      721 rnvarllgas leddpmcivl engeygdlnq ylqshiaets tvqtaktlsf gtlvymatqi
      781 asgmkhleem dfvhrdlatr nclvsrgytv kvsdlgsgrn ayaadyfrve grpplpirwm
      841 awesmlmgrh ttksdvwsfa vtlweiltfa reqpfeelpd hrivenatyf yqeddrriil
      901 plpkncpkdi yelmrecwhr ndvdrpnfre ihmflqrknl gykhadtndt
//
LOCUS       XP_034196137             922 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196137
VERSION     XP_034196137.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340246.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..922
                     /product="discoidin domain-containing receptor 2 isoform
                     X1"
                     /calculated_mol_wt=103982
     Region          37..187
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(76,107,115)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          623..911
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(635..638,641,643,665,667,697,713..716,772,776..777,
                     779,789..790)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     CDS             1..922
                     /gene="LOC117611851"
                     /coded_by="XM_034340246.2:470..3238"
                     /db_xref="GeneID:117611851"
ORIGIN      
        1 mktdclpiia kfwalltlfg ptrdvraldi rscnqslgme sgdipdsait asssyvtnvg
       61 prngrlrket aggawcpksq iergirewlq vdlsgphvit gvqsqgrydh grgqeyveey
      121 tleyrrpgfs ewrrykrwdn kevlagnsdt stvvshrlip pifasqiril phsehrrtvc
      181 lrielrgcrd tngvvsytip dsptpelsdi sydgkrqdnl ltdglgrlid gevgadnyrl
      241 dmgdgrgtgw iawmrdtfed dyvelvfefe vtwifeavhi ytnnyfsrdv qvfskadvwf
      301 sldgetyeee plsysyipdi vlenarnvsi vlherrakfl kihlyfaarw imisevtfdg
      361 tnpyenttee sasefsngei pmnpevdlnl qtitaagegq eylevligvl taiilllllv
      421 fviilllnrr qklqssptvl knpfgfainm kglllnltpg gmltetanhv spdipedgsm
      481 hesltmeqfn splvspqyks tyaivatses pkdlkdmngs eenvrldprp estigptscs
      541 ssptnsparh sqhyrtlqsy tsptaklnia assnhqrdvd qihskrwhta pkekhkipap
      601 vvswniapsm nkpykckeie ptniprqclr tteklgsrai geaivcetvg ledvvpdapr
      661 lvvarvptct sdiragstte qmrevrflss lsdpnvaril gvctmdpvpw tiieytelgd
      721 lahylqysvp ltgtlrpscn lkalsqscll ymgtqiasgm rflecknlvh kdlaarnclv
      781 grsytvkvtd iamcsdlykk dysdiggrpp apirwlpwes illdrytcss svwsfavtlw
      841 evmslarekp fqhltndqvi qnaehmyyga elqvylpkpt mcpeevyrmm cscwrrdets
      901 rptfkdiytf lksiiadyrp ga
//
LOCUS       XP_034196147             920 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196147
VERSION     XP_034196147.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340256.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..920
                     /product="discoidin domain-containing receptor 2 isoform
                     X2"
                     /calculated_mol_wt=103797
     Region          37..187
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(76,107,115)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          621..909
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(633..636,639,641,663,665,695,711..714,770,774..775,
                     777,787..788)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     CDS             1..920
                     /gene="LOC117611851"
                     /coded_by="XM_034340256.2:470..3232"
                     /db_xref="GeneID:117611851"
ORIGIN      
        1 mktdclpiia kfwalltlfg ptrdvraldi rscnqslgme sgdipdsait asssyvtnvg
       61 prngrlrket aggawcpksq iergirewlq vdlsgphvit gvqsqgrydh grgqeyveey
      121 tleyrrpgfs ewrrykrwdn kevlagnsdt stvvshrlip pifasqiril phsehrrtvc
      181 lrielrgcrd tngvvsytip dsptpelsdi sydgkrqdnl ltdglgrlid gevgadnyrl
      241 dmgdgrgtgw iawmrdtfed dyvelvfefe vtwifeavhi ytnnyfsrdv qvfskadvwf
      301 sldgetyeee plsysyipdi vlenarnvsi vlherrakfl kihlyfaarw imisevtfdg
      361 tnpyenttee sasefsngei pmnpevdlnl qttagegqey levligvlta iilllllvfv
      421 iilllnrrqk lqssptvlkn pfgfainmkg lllnltpggm ltetanhvsp dipedgsmhe
      481 sltmeqfnsp lvspqyksty aivatsespk dlkdmngsee nvrldprpes tigptscsss
      541 ptnsparhsq hyrtlqsyts ptaklniaas snhqrdvdqi hskrwhtapk ekhkipapvv
      601 swniapsmnk pykckeiept niprqclrtt eklgsraige aivcetvgle dvvpdaprlv
      661 varvptctsd iragstteqm revrflssls dpnvarilgv ctmdpvpwti ieytelgdla
      721 hylqysvplt gtlrpscnlk alsqscllym gtqiasgmrf lecknlvhkd laarnclvgr
      781 sytvkvtdia mcsdlykkdy sdiggrppap irwlpwesil ldrytcsssv wsfavtlwev
      841 mslarekpfq hltndqviqn aehmyygael qvylpkptmc peevyrmmcs cwrrdetsrp
      901 tfkdiytflk siiadyrpga
//
LOCUS       XP_076546044             781 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546044
VERSION     XP_076546044.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689929.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="discoidin domain-containing receptor 2 isoform
                     X3"
                     /calculated_mol_wt=88456
     Region          482..770
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(494..497,500,502,524,526,556,572..575,631,635..636,
                     638,648..649)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..781
                     /gene="LOC117611851"
                     /coded_by="XM_076689929.1:2620..4965"
                     /db_xref="GeneID:117611851"
ORIGIN      
        1 mhrtilfrls cmqikrgrys fnkrnrrsfl revrrklarp hrvtrltghg hgvvsytipd
       61 sptpelsdis ydgkrqdnll tdglgrlidg evgadnyrld mgdgrgtgwi awmrdtfedd
      121 yvelvfefev twifeavhiy tnnyfsrdvq vfskadvwfs ldgetyeeep lsysyipdiv
      181 lenarnvsiv lherrakflk ihlyfaarwi misevtfdgt npyenttees asefsngeip
      241 mnpevdlnlq titaagegqe ylevligvlt aiilllllvf viilllnrrq klqssptvlk
      301 npfgfainmk glllnltpgg mltetanhvs pdipedgsmh esltmeqfns plvspqykst
      361 yaivatsesp kdlkdmngse envrldprpe stigptscss sptnsparhs qhyrtlqsyt
      421 sptaklniaa ssnhqrdvdq ihskrwhtap kekhkipapv vswniapsmn kpykckeiep
      481 tniprqclrt teklgsraig eaivcetvgl edvvpdaprl vvarvptcts diragstteq
      541 mrevrflssl sdpnvarilg vctmdpvpwt iieytelgdl ahylqysvpl tgtlrpscnl
      601 kalsqsclly mgtqiasgmr flecknlvhk dlaarnclvg rsytvkvtdi amcsdlykkd
      661 ysdiggrppa pirwlpwesi lldrytcsss vwsfavtlwe vmslarekpf qhltndqviq
      721 naehmyygae lqvylpkptm cpeevyrmmc scwrrdetsr ptfkdiytfl ksiiadyrpg
      781 a
//
LOCUS       XP_034196158              97 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611862 [Osmia lignaria lignaria].
ACCESSION   XP_034196158
VERSION     XP_034196158.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340267.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..97
                     /product="uncharacterized protein LOC117611862"
                     /calculated_mol_wt=10404
     CDS             1..97
                     /gene="LOC117611862"
                     /coded_by="XM_034340267.2:89..382"
                     /db_xref="GeneID:117611862"
ORIGIN      
        1 mkfyltllil allaiihhpt aansvcpqen cldlskcddl vvggtcpqst dsccsivkme
       61 hrthcrhfgg ecldscaqtl qhtvvdcpad kvcctlv
//
LOCUS       XP_034196290             380 aa            linear   INV 03-SEP-2025
DEFINITION  L-lactate dehydrogenase [Osmia lignaria lignaria].
ACCESSION   XP_034196290
VERSION     XP_034196290.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340399.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196290.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..380
                     /product="L-lactate dehydrogenase"
                     /calculated_mol_wt=41881
     Region          66..377
                     /region_name="LDH_1"
                     /note="A subgroup of L-lactate dehydrogenases; cd05293"
                     /db_xref="CDD:133429"
     Site            order(66,68,229,232,234,251,256,313..315,318,345..346,349,
                     351..353)
                     /site_type="other"
                     /note="tetramer (dimer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:133429"
     Site            order(77..79,100,102,105,143..147,184,186,209,213,241,296,
                     300)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:133429"
     Site            order(81,86,108..109,112,114..115,217,219..220,226,
                     292..293,296..299)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:133429"
     Site            order(148,154,186,217,241,286,296)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:133429"
     CDS             1..380
                     /gene="LOC117611918"
                     /coded_by="XM_034340399.2:29..1171"
                     /db_xref="GeneID:117611918"
ORIGIN      
        1 mnrfrrtlpp tilkeifggn kekkkfmsad fgkdasagsd lqravsfvpp clkeellcky
       61 sepvrdcchk vtivgagmvg vaiansiifq risphvaivd afpkklegeg mdyshgsvyl
      121 gdpridydtd fcissnsrvv ilaagvrqvk gesrldlvkr nteilkniip tlvgyspnav
      181 flivsnpvdi lswvtwkvsg lpanriigsg thldsarfrf liaerleiap saihayivge
      241 hgdsqvplws gvnvagvqfr dilpniglet deerwneiak evvrlgptvr clkgysntai
      301 glstanivda ilsntqrvmp vstmiqghhe vchemflslp csigeqgvtn ivrmritehe
      361 kklfqtsakh vynvqkdmki
//
LOCUS       XP_034196302             332 aa            linear   INV 03-SEP-2025
DEFINITION  L-lactate dehydrogenase [Osmia lignaria lignaria].
ACCESSION   XP_034196302
VERSION     XP_034196302.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340411.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..332
                     /product="L-lactate dehydrogenase"
                     /calculated_mol_wt=35836
     Region          20..329
                     /region_name="LDH_1"
                     /note="A subgroup of L-lactate dehydrogenases; cd05293"
                     /db_xref="CDD:133429"
     Site            order(20,181,184,186,203,208,265..267,270,297..298,301,
                     303..305)
                     /site_type="other"
                     /note="tetramer (dimer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:133429"
     Site            order(29..31,52,54,57,95..99,136,138,161,165,193,248,252)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:133429"
     Site            order(33,38,60..61,64,66..67,169,171..172,178,244..245,
                     248..251)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:133429"
     Site            order(100,106,138,169,193,238,248)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:133429"
     CDS             1..332
                     /gene="LOC117611919"
                     /coded_by="XM_034340411.2:165..1163"
                     /db_xref="GeneID:117611919"
ORIGIN      
        1 mtsikarlls tvvqpistgr nkitvvgvgq vgmacaftll tnnvsseval vdvmadklkg
       61 emldlqhgsa flknakinas tdyastanss lcivtagarq regetrldlv qrntdifkgi
      121 ipqlvkyspn tillivsnpv diltyvawrl sglpknrvig sgtnldsarf rfllsqklnv
      181 aptschgwii gehgdtsvpv wsgvnvagvr lrdlnekvgt eedeehwsdl hrqvvdsaye
      241 viklkgytsw aiglsvanla samlrnsnqv havstmikgy hgidkevfls lpctlgeggv
      301 tcvvqqkltq delgnllssa namndvqkdl kf
//
LOCUS       XP_034196369             858 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatase 6 regulatory subunit 1-like protein fmt isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034196369
VERSION     XP_034196369.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340478.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196369.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..858
                     /product="phosphatase 6 regulatory subunit 1-like protein
                     fmt isoform X1"
                     /calculated_mol_wt=97269
     Region          128..508
                     /region_name="SAPS"
                     /note="SIT4 phosphatase-associated protein; pfam04499"
                     /db_xref="CDD:398279"
     CDS             1..858
                     /gene="fmt"
                     /coded_by="XM_034340478.2:338..2914"
                     /db_xref="GeneID:117611950"
ORIGIN      
        1 mfwannyvss phieallnke nvslydlmdq edilqecksq nkklveyltr sdvmeelvtl
       61 tteeppaeie erwrykhpni acelltcdvp tlnekladdd tllaklysfi dtdqplnpll
      121 asffsktigv lvarktdqnw ysyqytclkv ldflksrqtc vdlllhhlet saimdlvlkl
      181 vtqledtdmr qnilnwlhsq qlvqrlvkll ssnsesskha naaqllcdmi tvsrehqrts
      241 tkrtdpdpil ntlesadtvs llletiltge klessivggi qvlltllgqk annainegdi
      301 hgnsygdevt deqclrisna tlpyleqlhk llldppykpp vkttagvlec plgntrlhva
      361 klfttlmttd nvkiyeslve lgtfqtlldl ffkytwnnfl htqvqlclal aincdfkdtn
      421 nivynnifvk crlidrilea wdkndskqsa engirqgymg hlinianniv nqredsesld
      481 kflkdnlsse cldkwddlvn aklskinkth qifldemaqp fmtkssenpd eyssysqeef
      541 lqvqrveqfy tnyvgqhmts qfienfgfhd dqfndgddal hnsvvqlttl aftlseedld
      601 kredmfnkic qqkqkagled cstgvewgde geltfqtvvd krdwptkqqh ndsnssdede
      661 edpdhmhmei dltkfwgpte ppstgtalpe vnpwdvapse pvestgwanf dnfentvsme
      721 ntvivdnkvc deskertqdi tmdqnkieti sgneiaveai stndikieds ngsttsriqt
      781 ninhqntedn lysectadkn anpsenvdss enkgednvkp telvsatmqn ekssndhrvt
      841 csvknassea vlpptdak
//
LOCUS       XP_034196378             858 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatase 6 regulatory subunit 1-like protein fmt isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034196378
VERSION     XP_034196378.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340487.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196378.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..858
                     /product="phosphatase 6 regulatory subunit 1-like protein
                     fmt isoform X1"
                     /calculated_mol_wt=97269
     Region          128..508
                     /region_name="SAPS"
                     /note="SIT4 phosphatase-associated protein; pfam04499"
                     /db_xref="CDD:398279"
     CDS             1..858
                     /gene="fmt"
                     /coded_by="XM_034340487.2:338..2914"
                     /db_xref="GeneID:117611950"
ORIGIN      
        1 mfwannyvss phieallnke nvslydlmdq edilqecksq nkklveyltr sdvmeelvtl
       61 tteeppaeie erwrykhpni acelltcdvp tlnekladdd tllaklysfi dtdqplnpll
      121 asffsktigv lvarktdqnw ysyqytclkv ldflksrqtc vdlllhhlet saimdlvlkl
      181 vtqledtdmr qnilnwlhsq qlvqrlvkll ssnsesskha naaqllcdmi tvsrehqrts
      241 tkrtdpdpil ntlesadtvs llletiltge klessivggi qvlltllgqk annainegdi
      301 hgnsygdevt deqclrisna tlpyleqlhk llldppykpp vkttagvlec plgntrlhva
      361 klfttlmttd nvkiyeslve lgtfqtlldl ffkytwnnfl htqvqlclal aincdfkdtn
      421 nivynnifvk crlidrilea wdkndskqsa engirqgymg hlinianniv nqredsesld
      481 kflkdnlsse cldkwddlvn aklskinkth qifldemaqp fmtkssenpd eyssysqeef
      541 lqvqrveqfy tnyvgqhmts qfienfgfhd dqfndgddal hnsvvqlttl aftlseedld
      601 kredmfnkic qqkqkagled cstgvewgde geltfqtvvd krdwptkqqh ndsnssdede
      661 edpdhmhmei dltkfwgpte ppstgtalpe vnpwdvapse pvestgwanf dnfentvsme
      721 ntvivdnkvc deskertqdi tmdqnkieti sgneiaveai stndikieds ngsttsriqt
      781 ninhqntedn lysectadkn anpsenvdss enkgednvkp telvsatmqn ekssndhrvt
      841 csvknassea vlpptdak
//
LOCUS       XP_034196387             844 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatase 6 regulatory subunit 1-like protein fmt isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034196387
VERSION     XP_034196387.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196387.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="phosphatase 6 regulatory subunit 1-like protein
                     fmt isoform X2"
                     /calculated_mol_wt=95793
     Region          128..508
                     /region_name="SAPS"
                     /note="SIT4 phosphatase-associated protein; pfam04499"
                     /db_xref="CDD:398279"
     CDS             1..844
                     /gene="fmt"
                     /coded_by="XM_034340496.2:338..2872"
                     /db_xref="GeneID:117611950"
ORIGIN      
        1 mfwannyvss phieallnke nvslydlmdq edilqecksq nkklveyltr sdvmeelvtl
       61 tteeppaeie erwrykhpni acelltcdvp tlnekladdd tllaklysfi dtdqplnpll
      121 asffsktigv lvarktdqnw ysyqytclkv ldflksrqtc vdlllhhlet saimdlvlkl
      181 vtqledtdmr qnilnwlhsq qlvqrlvkll ssnsesskha naaqllcdmi tvsrehqrts
      241 tkrtdpdpil ntlesadtvs llletiltge klessivggi qvlltllgqk annainegdi
      301 hgnsygdevt deqclrisna tlpyleqlhk llldppykpp vkttagvlec plgntrlhva
      361 klfttlmttd nvkiyeslve lgtfqtlldl ffkytwnnfl htqvqlclal aincdfkdtn
      421 nivynnifvk crlidrilea wdkndskqsa engirqgymg hlinianniv nqredsesld
      481 kflkdnlsse cldkwddlvn aklskinkth qifldemaqp fmtkssenpd eyssysqeef
      541 lqvqrveqfy tnyvgqhmts qfienfgfhd dqfndgddal heedldkred mfnkicqqkq
      601 kagledcstg vewgdegelt fqtvvdkrdw ptkqqhndsn ssdedeedpd hmhmeidltk
      661 fwgpteppst gtalpevnpw dvapsepves tgwanfdnfe ntvsmentvi vdnkvcdesk
      721 ertqditmdq nkietisgne iaveaistnd ikiedsngst tsriqtninh qntednlyse
      781 ctadknanps envdssenkg ednvkptelv satmqnekss ndhrvtcsvk nasseavlpp
      841 tdak
//
LOCUS       XP_034180267            4923 aa            linear   INV 03-SEP-2025
DEFINITION  dynein heavy chain at 89D [Osmia lignaria lignaria].
ACCESSION   XP_034180267
VERSION     XP_034180267.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324376.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180267.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4923
                     /product="dynein heavy chain at 89D"
                     /calculated_mol_wt=567895
     Region          510..1180
                     /region_name="DHC_N1"
                     /note="Dynein heavy chain, N-terminal region 1; pfam08385"
                     /db_xref="CDD:462457"
     Region          1680..2084
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          1908..4565
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          2221..2547
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          4626..4920
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..4923
                     /gene="Dhc98D"
                     /coded_by="XM_034324376.2:129..14900"
                     /db_xref="GeneID:117604388"
ORIGIN      
        1 mtetenentk sdtegdedne lndlailsrh svlqmdsrlm wirnkvmkfl gitgqdlsfy
       61 kllnannrhf edkilnflil dlygvtnier kvvyfyatyt teivqqeidv werkmdsesk
      121 kirksrrkik kskqerkldp lallkqlems smtiesskes sstavsevie igdeedkede
      181 geddeeegey rveeeeeqda eeeregeeeq qegddkskem gsgepsvfsl kkeesksyds
      241 vsinfvpspe eehkyirtkk mvekvekvpv lhiicgeldg srndlhdvtl fyflrtkdeg
      301 vppfdsyqec deeitnylvv gslqgrflls mnrmlaqvfk plvehqfrgp qfaeirkept
      361 deeenltdil qarssilrrp sefrsvsiam skkfevssgd glvketeeme eekpaaqkks
      421 srlrtdytvs stfdqpsskt asaikdtddv rrsidrsknd ilsylkklit kvewtidrie
      481 gdllltmpdi mefhdpkvtd emleknkgvi dhfeevvmsw gghiqkvles fhtkvpedkg
      541 plaeieywqd retgllmlve qlktpiakri lgllnrvmsp iasnfeffys dlwkdytear
      601 dnnnflqtii rhfkliaess sfkaitqaip slteglrmiw ilsryyased kmmgllqris
      661 welcqnvirn latkelfklp leeiiektad astmlkswkl syldyrkeie dsgkgnrwef
      721 dqkqlfrete yiasvcdgln eianvlqdfy nifgphlksi isdpaqidti ikrvdrlitp
      781 ileadfnifd eynrdnweat miwffeevhf leneakffid ecfmtlisae dglevllkfk
      841 svktrqtiyq qllrkfdvim qqfskeismv egiynrgkrs pplltyhppm agaiywvrql
      901 fhrlkmpalt lqsipelkhs elkllafsqy veiakqmksf eeskyrtwad kaqfvvmntm
      961 kksilkmars epdkgllmfp geeitknlna iqmsrktksk rygstgstpm gsvqkgdvls
     1021 kpsfettssv gggskllpak ksdsqkdqkt svsssmekqg pqgakitwme fmsgsilvec
     1081 qlrfevnfew eifdiireae lmeqlgfela pivkeagiqk drlrtdvqat ervitqynqm
     1141 idkmdkadil llktnlldie khiqpgltrf twnslniqdy innckkllrg lisivdqvsr
     1201 mkddlnnrin telqtynlfi lppelydcel lpcktyfeei qnrrtellta mrkayqstsp
     1261 tliklenlvl gtstgsnsam qlfyekyekk ifssfiismt mnlvhfnksl msnkplfqvd
     1321 avlissevvl rpspgdiyni ilqnvknlle slklfprwmn gtcldckphk rnnsdqytsi
     1381 tffedvmsiq ivndniltiq gsahrltlec whylirwrky snlwtfdknl aakkfaatep
     1441 tlfhynekft fydnildevn emetrhdlyc irlnldplls gmkqhanewk qvlgtfllee
     1501 tikgmhdlks iieqyrseve ivitgldrft svmqaisdik ktaiqaevri styqetfrtf
     1561 yghhipypee deqmahklqh dweslylgal yrastlestc drfsemtqqq inhfleelke
     1621 fakdfeengp gsvgenldfg lvkmteygak iaqledrrqs minsellfdl patdyssflk
     1681 vkkdyegmel lydlykqqrn arddwsqtlw vnlnpqllid gmdhfirefr klskfvkels
     1741 igqalelnmk sfknsvplfi elkneamrer hwkelmkktg qyfdmdpdrf tlqnmfamel
     1801 gkyqdiakei vmnavkelai erglkelaev wknmeftvvk hmkgtedrgf ilgpveelnq
     1861 vlednmmnvn gmaasqfigp flntvqkwei tmhtiaenle lwiqlqrkwl ylegifvggd
     1921 irlqlpdeak kfddidktfk kvmadtskrl nvldccting rkeefeamih gldkcqkslt
     1981 dylnskrvvf srftflsdde lltilgsgqp taiqehvgkm fdnlekfrfd ysnsdrvitt
     2041 aliscegeam efrnavlaeg kieewmgval eemkksnryl tkkavydygk vrrprtewmf
     2101 dfqgmmilaa dqiwwtaeve nvfekiskgq myamkeylsq lnnqlnevvt lmggetltnn
     2161 drkkfdmvlt ldvhmrdiie gfvrdtimdp sefewesqlr fywlrdpdnv wihqctgtfe
     2221 ygyeymglng rlvvtpltdr iyltitqals mhlggapagp agtgktettk dlakalgllc
     2281 ivtncgegmd yvaigktlsg laqcgawgcf defnridisv lsvistqlqt irsalmanas
     2341 rfmfegqdiv ldskvgifit mnpgyagrte lpesvkalfr pvvcivpdne licqiklfss
     2401 gfltakvlak kmtvlynlar eqlskqnhyd fglralksvl nmagqlkrts gelpenvvlm
     2461 ralrdmnlpk fiyddvplfl gliadlfpdl ncprvhypdf neavnavlee qrysvlseqv
     2521 dkvvqlyevm mtrhstmvvg ptgggktvvi dtlckaqtrl dkptklytln pkactvnely
     2581 gvldpvtrdw tdgllskifr evnkpmgtgk derkyilfdg dvdalwienm nsvmddnkll
     2641 tlanqerikm qnhcsllfev gdlqyaspat vsragmvyvd pknlgyhpym ekwmrgkies
     2701 drellsgmye kyidgaikli iegmlglqav tplkmiipqt glnmvtqlcy vfdslleisd
     2761 kaspmieneg demtvieplf drkeiieamy iqacywslga tlvinekprf deyvkktagf
     2821 mlvqdtvekp atiryipvte stlydyyldl nkniwipwrf lvpeyvhdre khfseilvpt
     2881 vdtsraiwfi qtmsdldrpa llvgetgtsk taiineflrn lstdkykqll infssrtssm
     2941 dvqrnieavv ekrtreiygp ppgkklllfi ddmnmplvdt ygtqqpiall kflfekggfy
     3001 drekdlsfky mndmcylaam gkpgggrnev dprfismfsv ynvtfpsdet ldyiyrsils
     3061 ghlqifseev qaiaadlvqi tlnlyqiitk ellptpskfh yifnmrdlsr itagllqshp
     3121 dylssvkqiv rlwrneftrv icdrlisked kdlvdgyike kieerweeed setvayamrd
     3181 pllfgdfrna inedeprfye dlldyeavyn lffeifedyn ernatilhmv lfndaldhlt
     3241 rvhralrmhr ghvlvigigg sgkrsvinla syaagyrtfe isltrgynea sfredmknly
     3301 nmvgvnnqki vfvftsshii nesflelvnn mlmtgvvpsl ftdedkdeiv nscrnaakea
     3361 gfgvtkesvw ayfvktslen lrialsmsps gdalrtrcrn ypglvnstti dwifpwpeqa
     3421 lvavanvtlr dnpnvpqdyt eqlvqhmvyv hktvreytid fqtklrrrny vtpkhyldfi
     3481 nsylnllvet kdyinsqcdr lsggiqkiae asvtlndlne ilavqrvkva dqtknceqll
     3541 tsigestdia mekkklseek rkeiedqqkv inkeeteakq vlaeaqpald aaraalsele
     3601 kaditeirsf atppepvqiv secvamlrgv kdiswkgakg mmsdpaflri lqemncdkit
     3661 lkqqqsvkah lkkttkldqm etiskagfgl ykfviavldy cavyrevkpk iervkaleae
     3721 serarkaler eerelkklek aiselnakye kameerqklq eetdvlqrrl iaadklingl
     3781 ssenerwkke lenlheqivk ivgdcvlsag flaycapfsy eyrnkmlyed wknsviekki
     3841 pftetfries qlsndvqist wtseglppde lsvqngiltt rasrfplcid pqqqalnwik
     3901 kkeqkknlki lsfidadflk qvelaikygl pvlfqdvdev dpvldnvlsk niqvsggrmf
     3961 vllgdkevdy dpkfrlyltt kmsnpifdpa lyakatiiny mvtiggledq llsvvvrter
     4021 pdieeqrenl iietsenknl lqqledslll eiasnkgnml dnielvetle ntkssayevm
     4081 tklylgevta advnklrdgy rpvakrgail ffvladmamv nsmyqyslis yvevfiyslr
     4141 kalpdptlqq rlkniipmlt knvydygctg iferhkllfs fqicikleqd qekltqgeld
     4201 ffvkgsiale kaskinptdw lpatgwadvl klsndfpdvf gqlpneleeq leewqkwyds
     4261 dapesenypl dyssklkpfe klmlircfri drvyrgivny iseimgeefi tpphisfeli
     4321 feqstptmpi vfilspgsdp sselmkladg ygcgggkfky lslgqgqeki avelleiavn
     4381 rgqwlmfqnc hlllsftrel ekllenvgkp hpdfrlwltt dptpnfpigi lqqslkvvte
     4441 ppsglklnle ntylkmtpql iescphsayk hliyvlafyh avvqerrryd kigwninydf
     4501 nesdfnvctt ilntyltkal stkeiripwn slkyligevm yggrvidnyd rrvsqtymde
     4561 yfgdflfdkf qpfhfyrder vdyvippege yddylnfike lplvntpevf glhpnaeigy
     4621 ftyavkemws nlielqpqte vsgtgiskde fidnmaneil nkvptefdii kikknygisv
     4681 tptiivlfqe lerfnklirt mkrtltqlrk avageigmdt tlesistavy ngvlpkqwak
     4741 lapdtkktla gwmehfekri qqynnwagtn epivlwlagl hipetylaal iqmscrrnnw
     4801 sldhslmyta vsrytipeki edrpdegcyv sglylegarw dleeqclkrs hpkilveelp
     4861 vlivvpveah rirlqntfkt pvyttsnrrn amgvglvfea nlatsehish wvlqgvclil
     4921 ntd
//
LOCUS       XP_034173981            1265 aa            linear   INV 03-SEP-2025
DEFINITION  tonsoku-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034173981
VERSION     XP_034173981.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173981.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1265
                     /product="tonsoku-like protein"
                     /calculated_mol_wt=142918
     Region          23..51
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(24,27..28,31..32,34,64,67..68,71..72,74..75,104,
                     107..108,111..112,122)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          28..371
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          62..96
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          103..137
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          195..231
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          238..261
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          306..333
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          338..369
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <506..625
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          510..540
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(511,515..516,519..521,523..524,528,531,540,542,544,
                     548..549,552..554,556..557,561,564,573,578,580,584..585,
                     588..590,592..593,597,600)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          542..573
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          578..608
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <949..1201
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          966..988
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          989..1015
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          1016..1040
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1041..1068
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1069..1090
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1091..1124
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1145..1169
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          1170..1195
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..1265
                     /gene="LOC117601399"
                     /coded_by="XM_034318090.2:118..3915"
                     /db_xref="GeneID:117601399"
ORIGIN      
        1 mdvdrltrkk krvkrdgnlq qlaevtkelg dvyfetgrfe dalqeykeql evcniledkl
       61 niavahrmig eiyanlgtye ealkhqnlyl egakeiknll eeqrayatlg rtyfcwaesl
      121 pevenksdal ksskkayvks irlcneledt nidlkelitm rarlllnlgl vleaqkdhqe
      181 avdlmekaaa lcqthnlked yhrtqialgg iyerqcnyel alkhfenate vdntslkaea
      241 rlfqaelllk merwhesrki lvslyvsskl penlkhqvek clrivaalht teealnaees
      301 tsgklklyet lgdasvaaqc fekaidyykk mlecaeemks drigaallsl aqtlkdvgry
      361 dealefaqre lisctdprei crsalfladl liankaadek ieetynlaln nakdssnisl
      421 easvlkerln ffkstgkteg ietlverlke lekystdtds eseseeqiga dirledlsdv
      481 eaelqakgns rvsrkrtrkk smaikrnekg etqlhvacin gnietaekll eaghptnard
      541 hfgwtplhea anhgyveiae lllkhgadvn dpgslmcqgv tplhdaascg nirmmrllmq
      601 yganvelktn eddtvldcle qwrnrvdyls sedqeeydem ynmlsaiipa sqrknfkrsy
      661 ksphnsktyi vdkdntveki sagedykrti aslkrrndpi gaplsrsnsn vnpllnseev
      721 llddwleddi senissrrcs delsiikrks ssgdidsekt lkrqktsnes fvtkekefdl
      781 ieedsnescd tvvtcssnas ksrrkkqqvs llsigfskns vsrtpspvnp sptefefret
      841 gaiksiilnv svegkvfktq vefsntakpl iqdiltdikt kfyndsgcka kfelktvsgi
      901 ainnnnllti lnegdviknl qceiieletp siverygiic knynidvres vskclkscen
      961 tfilrlkqed isknefisll ktleyqknvq ildlsggely elgkvlndci lklstlqelc
     1021 lqgcdvdsnc lnkldklpvq lkhldlsynp lgpasqetls kllrpliqlq tlnlrycqln
     1081 nfqfilnncs lvnldiswns fnenglgtsl qrqllnlnls ntasssefnl vkniftstsy
     1141 sflnleclel aacdlmdtdv rnilsqvsnl sklvlrgnkn vgvkslhlll nhtptlryid
     1201 vsgcksitey pdveifienp eictlivnmy pevcdcwicl wrgngvvkrl phnlvmfkpi
     1261 sgigg
//
LOCUS       XP_034180280            1118 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604393 [Osmia lignaria lignaria].
ACCESSION   XP_034180280
VERSION     XP_034180280.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324389.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180280.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="uncharacterized protein LOC117604393"
                     /calculated_mol_wt=125952
     Region          145..197
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..1118
                     /gene="LOC117604393"
                     /coded_by="XM_034324389.2:37..3393"
                     /db_xref="GeneID:117604393"
ORIGIN      
        1 mvelgprrrl grrsrtssqc igcfrlagtf gaeqlvetha tcnnmpirlr tlclvtlvlc
       61 niadslehei prrsflsrra idgtrgrkyf dpeeegafds ygsaggqydp yeektdlsdi
      121 rrnvpgepgv dypayttlpq tgftcegrsr gyyadevagc qvfhvchdvl vssflcpigs
      181 ifsqklltcd wwtkvdcsss gkyidvnrns yqqdddemir nayamislqs gtdvtkdglv
      241 dpdrtgsvvd yqraparipd yssldttgnd lrnnyedyss svnrnflppy qskdrkiess
      301 psyqerfypp eksrspyqds piirvqnikd pgyrdaernk hfqenyrrpn efsnqfqpsy
      361 aptvptvttt trrfysptvp ttfrpstlay nkldqeidss dyyfshsrtn sfvtpasrnv
      421 edqgvkksea grkandysqq dsyeddyddv nseeerqttp sgerfrirva ddyrvnrtgl
      481 lfgqvyrgfd rarddeeile dietrgsigl ghalgesfrg isvqqqnpvn dglkdgeaev
      541 rahhrytltr pessissgnr eeetteqyqr ndqreqgiil kedlvsfttp sviestikat
      601 tnlhefnsss vpvnsknste essnqkhsik rvdkflrppe esrtkfqinv pdlsevstll
      661 irystvddgs srttelpsfr ntysddyidh ptahdesvsv trnyedirgs gepgrsestt
      721 dinrfretvt prldkepsts pvtklpiies srstsksgta edetpttlik pirpetlkqi
      781 eesieknnsp yhvtltvnkd eeliptahdl igkliaqqgk esstvndklh eleiiksvep
      841 klhptdppts pmstidthhe sntgnnhsri eqnssrskdf drisllqlms ellkldrapr
      901 pfslsdayts dletqpevvs gssasqtiak evnlnmqfqe evtekltess tepvvrstdp
      961 rlkdhildql aetfgepllk tdrplfdlpq knesddflkg lptvnvdnhq esssfrttle
     1021 tsdrfssrss skantiittt sepgttlttd sgktvvktef vpsigfsfdt drgreeyvea
     1081 vlggliepea aesekkeavi qqtsqetlse knetskdv
//
LOCUS       XP_034180290             887 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604403 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180290
VERSION     XP_034180290.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324399.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180290.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..887
                     /product="uncharacterized protein LOC117604403 isoform X1"
                     /calculated_mol_wt=101272
     Region          8..394
                     /region_name="TMEM151"
                     /note="TMEM151 family; pfam14857"
                     /db_xref="CDD:464349"
     CDS             1..887
                     /gene="LOC117604403"
                     /coded_by="XM_034324399.2:417..3080"
                     /db_xref="GeneID:117604403"
ORIGIN      
        1 mnreneeqrp vsqtlcgalq kepnckclvl svliygclaa vtwcrcanvt kvvinysryp
       61 ikstrhvspc ddgyiyipva fmgmlylvyl vecyhspiri dllhaesqds vlsklsqlks
      121 aqptiwwkav syhyvrrkrq itryrngdny tttqvyyeri nthaatsfyy ydycgvkdis
      181 kelildpkvp itkinlskgf afsnmrsate feearsrffa eqelrddyme mregldlgyn
      241 lnpttlvavl gnpwftnryv ywclsallls wplrviieyk tqyadyqitk lfginydtps
      301 gsepihasms qqtinqpgsy mlapsyseal lmdpapdqrp qdpsitemvp syseallyqr
      361 aeqgctmnde tnnenpnhpq eqprecscpc hsetsttegt rpeedvgqss sarepliqtt
      421 idvyppvcty vsqtetgrcg scgkllveaq lenpevsrne htpgssssgi hnprrelmss
      481 vmprdmsepn lrfrselmet dtkflringq slrnileseq eeefneervr gseqkrgerl
      541 rfslcsldea ssqsrtvdas rgaipkrtav rsrvianpms netcplpdsk tyfclksilk
      601 qnnrrytlit arelqnlsdq eeeevdrrrr vrssyhegci pvcasdiegr fnlfsrsres
      661 ldavlgkkrk qpgnvsndkt edismckrdn nrtlifaspi qevcpgdpfg gkdvvrtrqe
      721 vdctpteetp shtvlgqlgr sltcdrkssr rrfqdarrqr ysdssqpsaf scppdrqhpy
      781 pyafsastna mdaidfgkne sqvrisqhat sfppyegstr nekttnddtd rvssentfkg
      841 eltrslterr akparhdvsi rrsftgrved yrtdnnkwtn lnmetsl
//
LOCUS       XP_034180319             886 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604403 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180319
VERSION     XP_034180319.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324428.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180319.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..886
                     /product="uncharacterized protein LOC117604403 isoform X2"
                     /calculated_mol_wt=101143
     Region          8..393
                     /region_name="TMEM151"
                     /note="TMEM151 family; pfam14857"
                     /db_xref="CDD:464349"
     CDS             1..886
                     /gene="LOC117604403"
                     /coded_by="XM_034324428.2:417..3077"
                     /db_xref="GeneID:117604403"
ORIGIN      
        1 mnreneeqrp vsqtlcgalq kepnckclvl svliygclaa vtwcrcanvt kvvinysryp
       61 ikstrhvspc ddgyiyipva fmgmlylvyl vecyhspiri dllhaesqds vlsklsqlks
      121 aqptiwwkav syhyvrrkrq itryrngdny tttqvyyeri nthaatsfyy ydycgvkdis
      181 kelildpkvp itkinlskgf afsnmrsate feearsrffa eqlrddymem regldlgynl
      241 npttlvavlg npwftnryvy wclsalllsw plrviieykt qyadyqitkl fginydtpsg
      301 sepihasmsq qtinqpgsym lapsyseall mdpapdqrpq dpsitemvps yseallyqra
      361 eqgctmndet nnenpnhpqe qprecscpch setsttegtr peedvgqsss arepliqtti
      421 dvyppvctyv sqtetgrcgs cgkllveaql enpevsrneh tpgssssgih nprrelmssv
      481 mprdmsepnl rfrselmetd tkflringqs lrnileseqe eefneervrg seqkrgerlr
      541 fslcsldeas sqsrtvdasr gaipkrtavr srvianpmsn etcplpdskt yfclksilkq
      601 nnrrytlita relqnlsdqe eeevdrrrrv rssyhegcip vcasdiegrf nlfsrsresl
      661 davlgkkrkq pgnvsndkte dismckrdnn rtlifaspiq evcpgdpfgg kdvvrtrqev
      721 dctpteetps htvlgqlgrs ltcdrkssrr rfqdarrqry sdssqpsafs cppdrqhpyp
      781 yafsastnam daidfgknes qvrisqhats fppyegstrn ekttnddtdr vssentfkge
      841 ltrslterra kparhdvsir rsftgrvedy rtdnnkwtnl nmetsl
//
LOCUS       XP_034180329             734 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604403 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180329
VERSION     XP_034180329.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324438.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180329.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..734
                     /product="uncharacterized protein LOC117604403 isoform X3"
                     /calculated_mol_wt=83575
     Region          <2..241
                     /region_name="TMEM151"
                     /note="TMEM151 family; pfam14857"
                     /db_xref="CDD:464349"
     CDS             1..734
                     /gene="LOC117604403"
                     /coded_by="XM_034324438.2:208..2412"
                     /db_xref="GeneID:117604403"
ORIGIN      
        1 mvyyerinth aatsfyyydy cgvkdiskel ildpkvpitk inlskgfafs nmrsatefee
       61 arsrffaeqe lrddymemre gldlgynlnp ttlvavlgnp wftnryvywc lsalllswpl
      121 rviieyktqy adyqitklfg inydtpsgse pihasmsqqt inqpgsymla psyseallmd
      181 papdqrpqdp sitemvpsys eallyqraeq gctmndetnn enpnhpqeqp recscpchse
      241 tsttegtrpe edvgqsssar epliqttidv yppvctyvsq tetgrcgscg kllveaqlen
      301 pevsrnehtp gssssgihnp rrelmssvmp rdmsepnlrf rselmetdtk flringqslr
      361 nileseqeee fneervrgse qkrgerlrfs lcsldeassq srtvdasrga ipkrtavrsr
      421 vianpmsnet cplpdsktyf clksilkqnn rrytlitare lqnlsdqeee evdrrrrvrs
      481 syhegcipvc asdiegrfnl fsrsreslda vlgkkrkqpg nvsndktedi smckrdnnrt
      541 lifaspiqev cpgdpfggkd vvrtrqevdc tpteetpsht vlgqlgrslt cdrkssrrrf
      601 qdarrqrysd ssqpsafscp pdrqhpypya fsastnamda idfgknesqv risqhatsfp
      661 pyegstrnek ttnddtdrvs sentfkgelt rslterrakp arhdvsirrs ftgrvedyrt
      721 dnnkwtnlnm etsl
//
LOCUS       XP_034180341             749 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604420 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180341
VERSION     XP_034180341.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324450.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180341.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..749
                     /product="uncharacterized protein LOC117604420 isoform X1"
                     /calculated_mol_wt=84169
     CDS             1..749
                     /gene="LOC117604420"
                     /coded_by="XM_034324450.2:220..2469"
                     /db_xref="GeneID:117604420"
ORIGIN      
        1 mpqipkhrki krvtvltkkt qflrnylklk synplceklr snnyslakal skekektqll
       61 fsqnvalvae vqdlgsacsk ssavisnvlk nakdmltmiv tmtgyltnti stcqefvass
      121 panlrmssns tgtasilgdg srrlspkapt kgvvkpmvsg htitkptinl srvnmqhinn
      181 ssslsiiqev tsppgnresn sprssghvtm rqhrnedarv yrmperlkva sprlsdeser
      241 rlskrsnrrs grisgrlsgs lsksrklsvg nstpnsirtr nsienfenig nprvklndvs
      301 kllqnsqsin irmltenrns ernetlennv svnledsqtn setpesspdn sknnsnktig
      361 neneeigknk dgtsktqeni srtlitsnle dplegpswly nnsssvpsik yniqhtfhsf
      421 gnkdkktdev nisnndsmdt tlkatelsdp sdnensmqet srlsitnkre rynlkkdkei
      481 nnrneyqvvq rninndtfdn tistdltftn edeykvenea aenfsnfvtq rrvclrdede
      541 ddftlmymrq pnmnfdisdl klpvleqsal kpivlvepep eittsiklip qigplpsasn
      601 dslnesifdr stvklpvlnd nenkkkkimk vknrtsress efvestppgd gkstrkktnk
      661 svnkdpsaak vvlqklneyd vksrtpspev slsqdstksl spysrmenss dsensntsin
      721 smyalnrprr kraptvfhep nlkaklrrn
//
LOCUS       XP_034180351             749 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604420 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180351
VERSION     XP_034180351.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324460.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180351.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..749
                     /product="uncharacterized protein LOC117604420 isoform X1"
                     /calculated_mol_wt=84169
     CDS             1..749
                     /gene="LOC117604420"
                     /coded_by="XM_034324460.2:192..2441"
                     /db_xref="GeneID:117604420"
ORIGIN      
        1 mpqipkhrki krvtvltkkt qflrnylklk synplceklr snnyslakal skekektqll
       61 fsqnvalvae vqdlgsacsk ssavisnvlk nakdmltmiv tmtgyltnti stcqefvass
      121 panlrmssns tgtasilgdg srrlspkapt kgvvkpmvsg htitkptinl srvnmqhinn
      181 ssslsiiqev tsppgnresn sprssghvtm rqhrnedarv yrmperlkva sprlsdeser
      241 rlskrsnrrs grisgrlsgs lsksrklsvg nstpnsirtr nsienfenig nprvklndvs
      301 kllqnsqsin irmltenrns ernetlennv svnledsqtn setpesspdn sknnsnktig
      361 neneeigknk dgtsktqeni srtlitsnle dplegpswly nnsssvpsik yniqhtfhsf
      421 gnkdkktdev nisnndsmdt tlkatelsdp sdnensmqet srlsitnkre rynlkkdkei
      481 nnrneyqvvq rninndtfdn tistdltftn edeykvenea aenfsnfvtq rrvclrdede
      541 ddftlmymrq pnmnfdisdl klpvleqsal kpivlvepep eittsiklip qigplpsasn
      601 dslnesifdr stvklpvlnd nenkkkkimk vknrtsress efvestppgd gkstrkktnk
      661 svnkdpsaak vvlqklneyd vksrtpspev slsqdstksl spysrmenss dsensntsin
      721 smyalnrprr kraptvfhep nlkaklrrn
//
LOCUS       XP_034180359             748 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604420 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180359
VERSION     XP_034180359.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324468.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180359.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..748
                     /product="uncharacterized protein LOC117604420 isoform X2"
                     /calculated_mol_wt=84098
     CDS             1..748
                     /gene="LOC117604420"
                     /coded_by="XM_034324468.2:221..2467"
                     /db_xref="GeneID:117604420"
ORIGIN      
        1 mpqipkhrki krvtvltkkt qflrnylklk synplceklr snnyslakal skekektqll
       61 fsqnvalvae vqdlgsacsk ssavisnvlk nakdmltmiv tmtgyltnti stcqefvass
      121 panlrmssns tgtsilgdgs rrlspkaptk gvvkpmvsgh titkptinls rvnmqhinns
      181 sslsiiqevt sppgnresns prssghvtmr qhrnedarvy rmperlkvas prlsdeserr
      241 lskrsnrrsg risgrlsgsl sksrklsvgn stpnsirtrn sienfenign prvklndvsk
      301 llqnsqsini rmltenrnse rnetlennvs vnledsqtns etpesspdns knnsnktign
      361 eneeigknkd gtsktqenis rtlitsnled plegpswlyn nsssvpsiky niqhtfhsfg
      421 nkdkktdevn isnndsmdtt lkatelsdps dnensmqets rlsitnkrer ynlkkdkein
      481 nrneyqvvqr ninndtfdnt istdltftne deykveneaa enfsnfvtqr rvclrdeded
      541 dftlmymrqp nmnfdisdlk lpvleqsalk pivlvepepe ittsiklipq igplpsasnd
      601 slnesifdrs tvklpvlndn enkkkkimkv knrtsresse fvestppgdg kstrkktnks
      661 vnkdpsaakv vlqklneydv ksrtpspevs lsqdstksls pysrmenssd sensntsins
      721 myalnrprrk raptvfhepn lkaklrrn
//
LOCUS       XP_034180366             744 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604420 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180366
VERSION     XP_034180366.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324475.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180366.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..744
                     /product="uncharacterized protein LOC117604420 isoform X3"
                     /calculated_mol_wt=83783
     CDS             1..744
                     /gene="LOC117604420"
                     /coded_by="XM_034324475.2:218..2452"
                     /db_xref="GeneID:117604420"
ORIGIN      
        1 mpqipkhrki krvtvltkkt qflrnylklk synplceklr snnyslakal skekektqll
       61 fsqnvalvae vqdlgsacsk ssavisnvlk nakdmltmiv tmtgyltnti stcqefvass
      121 panlrmssns tgrdgsrrls pkaptkgvvk pmvsghtitk ptinlsrvnm qhinnsssls
      181 iiqevtsppg nresnsprss ghvtmrqhrn edarvyrmpe rlkvasprls deserrlskr
      241 snrrsgrisg rlsgslsksr klsvgnstpn sirtrnsien fenignprvk lndvskllqn
      301 sqsinirmlt enrnsernet lennvsvnle dsqtnsetpe sspdnsknns nktignenee
      361 igknkdgtsk tqenisrtli tsnledpleg pswlynnsss vpsikyniqh tfhsfgnkdk
      421 ktdevnisnn dsmdttlkat elsdpsdnen smqetsrlsi tnkrerynlk kdkeinnrne
      481 yqvvqrninn dtfdntistd ltftnedeyk veneaaenfs nfvtqrrvcl rdededdftl
      541 mymrqpnmnf disdlklpvl eqsalkpivl vepepeitts iklipqigpl psasndslne
      601 sifdrstvkl pvlndnenkk kkimkvknrt sressefves tppgdgkstr kktnksvnkd
      661 psaakvvlqk lneydvksrt pspevslsqd stkslspysr menssdsens ntsinsmyal
      721 nrprrkrapt vfhepnlkak lrrn
//
LOCUS       XP_034180376             704 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604420 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180376
VERSION     XP_034180376.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180376.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..704
                     /product="uncharacterized protein LOC117604420 isoform X4"
                     /calculated_mol_wt=79118
     CDS             1..704
                     /gene="LOC117604420"
                     /coded_by="XM_034324485.2:223..2337"
                     /db_xref="GeneID:117604420"
ORIGIN      
        1 mpqipkhrki krvtvltkkt qflrnylklk synplceklr snnyslakal skekektqll
       61 fsqnvalvae vqdlgsacsk ssavisnvlk nakdmltmiv tmtgyltnti stcqefvass
      121 panlrmssns tgtasilgdg srrlspkapt kgvvkpmvsg htitkptinl srvnmqhinn
      181 ssslsiiqev tsppgnresn sprssghvtm rqhrnedarv yrmperlkva sprlsdeser
      241 rlskrsnrrs grisgrlsgs lsksrklslt enrnsernet lennvsvnle dsqtnsetpe
      301 sspdnsknns nktignenee igknkdgtsk tqenisrtli tsnledpleg pswlynnsss
      361 vpsikyniqh tfhsfgnkdk ktdevnisnn dsmdttlkat elsdpsdnen smqetsrlsi
      421 tnkrerynlk kdkeinnrne yqvvqrninn dtfdntistd ltftnedeyk veneaaenfs
      481 nfvtqrrvcl rdededdftl mymrqpnmnf disdlklpvl eqsalkpivl vepepeitts
      541 iklipqigpl psasndslne sifdrstvkl pvlndnenkk kkimkvknrt sressefves
      601 tppgdgkstr kktnksvnkd psaakvvlqk lneydvksrt pspevslsqd stkslspysr
      661 menssdsens ntsinsmyal nrprrkrapt vfhepnlkak lrrn
//
LOCUS       XP_034180389             694 aa            linear   INV 03-SEP-2025
DEFINITION  fused lobes isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180389
VERSION     XP_034180389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..694
                     /product="fused lobes isoform X1"
                     /calculated_mol_wt=78696
     Region          152..274
                     /region_name="Glycohydro_20b2"
                     /note="beta-acetyl hexosaminidase like; pfam14845"
                     /db_xref="CDD:434261"
     Region          301..675
                     /region_name="GH20_HexA_HexB-like"
                     /note="Beta-N-acetylhexosaminidases catalyze the removal
                     of beta-1,4-linked N-acetyl-D-hexosamine residues from the
                     non-reducing ends of N-acetyl-beta-D-hexosaminides
                     including N-acetylglucosides and N-acetylgalactosides. The
                     hexA and hexB genes encode the...; cd06562"
                     /db_xref="CDD:119332"
     Site            order(312,341,395,465..466,520,544,571,573,621,623)
                     /site_type="active"
                     /db_xref="CDD:119332"
     CDS             1..694
                     /gene="fdl"
                     /coded_by="XM_034324498.2:141..2225"
                     /db_xref="GeneID:117604439"
ORIGIN      
        1 mpeafirrsl fhrgtyfhdf daflssyver larmvgsvps gwmrkillfl vlmtgvllia
       61 myahapplvs lqpfssrrlk elqrglvtfl vgnestkrpe erlyeyleep stfqspwswa
      121 cvagrcerra vrssrtslas cialcggntr llwprptgnv vlgddsviih lrqiefvtvn
      181 tsdqetknll ehakdifign irslvkvans ksrpgvdtfl iylsagngra vgpnldtdes
      241 ytlelipkgr tlearingks yfgvrhglet lgqmiwwdet agregalrvl shatvedkpm
      301 fgyrgllvdt grqffsierl krvidgmaas klntfhwhlt dsqsfpfdsa qfpemarwga
      361 ysgdqiytpd dvkdladyar irgirvlvei dspahagagw qwgteygyge lalcvdqqpw
      421 ssycgepncg qlnpinehsy rileglyrel ldlteirdiv hlggdevnld cwaqygnita
      481 amqaqnmtdh hamwaefetk imqrlvkanh dqtpkavilw sspltkrpyi imyfdpkihv
      541 iqswggstwp etpdlledgf rvilshvdaw yldcgfgrwr etgeaacgey rtwqtvynhr
      601 pwrdypqqhl hlvlggeaai wseqtgdasl gprlwprasa laerlwsdlp tngystdesv
      661 ytrlaahmev ltsrglktea mwpqwcsqnp gkcl
//
LOCUS       XP_034180396             661 aa            linear   INV 03-SEP-2025
DEFINITION  fused lobes isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034180396
VERSION     XP_034180396.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="fused lobes isoform X2"
                     /calculated_mol_wt=74677
     Region          119..241
                     /region_name="Glycohydro_20b2"
                     /note="beta-acetyl hexosaminidase like; pfam14845"
                     /db_xref="CDD:434261"
     Region          268..642
                     /region_name="GH20_HexA_HexB-like"
                     /note="Beta-N-acetylhexosaminidases catalyze the removal
                     of beta-1,4-linked N-acetyl-D-hexosamine residues from the
                     non-reducing ends of N-acetyl-beta-D-hexosaminides
                     including N-acetylglucosides and N-acetylgalactosides. The
                     hexA and hexB genes encode the...; cd06562"
                     /db_xref="CDD:119332"
     Site            order(279,308,362,432..433,487,511,538,540,588,590)
                     /site_type="active"
                     /db_xref="CDD:119332"
     CDS             1..661
                     /gene="fdl"
                     /coded_by="XM_034324505.2:531..2516"
                     /db_xref="GeneID:117604439"
ORIGIN      
        1 mvgsvpsgwm rkillflvlm tgvlliamya happlvslqp fssrrlkelq rglvtflvgn
       61 estkrpeerl yeyleepstf qspwswacva grcerravrs srtslascia lcggntrllw
      121 prptgnvvlg ddsviihlrq iefvtvntsd qetknlleha kdifignirs lvkvansksr
      181 pgvdtfliyl sagngravgp nldtdesytl elipkgrtle aringksyfg vrhgletlgq
      241 miwwdetagr egalrvlsha tvedkpmfgy rgllvdtgrq ffsierlkrv idgmaaskln
      301 tfhwhltdsq sfpfdsaqfp emarwgaysg dqiytpddvk dladyarirg irvlveidsp
      361 ahagagwqwg teygygelal cvdqqpwssy cgepncgqln pinehsyril eglyrelldl
      421 teirdivhlg gdevnldcwa qygnitaamq aqnmtdhham waefetkimq rlvkanhdqt
      481 pkavilwssp ltkrpyiimy fdpkihviqs wggstwpetp dlledgfrvi lshvdawyld
      541 cgfgrwretg eaacgeyrtw qtvynhrpwr dypqqhlhlv lggeaaiwse qtgdaslgpr
      601 lwprasalae rlwsdlptng ystdesvytr laahmevlts rglkteamwp qwcsqnpgkc
      661 l
//
LOCUS       XP_034180405             661 aa            linear   INV 03-SEP-2025
DEFINITION  fused lobes isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034180405
VERSION     XP_034180405.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324514.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="fused lobes isoform X3"
                     /calculated_mol_wt=74789
     Region          119..241
                     /region_name="Glycohydro_20b2"
                     /note="beta-acetyl hexosaminidase like; pfam14845"
                     /db_xref="CDD:434261"
     Region          268..642
                     /region_name="GH20_HexA_HexB-like"
                     /note="Beta-N-acetylhexosaminidases catalyze the removal
                     of beta-1,4-linked N-acetyl-D-hexosamine residues from the
                     non-reducing ends of N-acetyl-beta-D-hexosaminides
                     including N-acetylglucosides and N-acetylgalactosides. The
                     hexA and hexB genes encode the...; cd06562"
                     /db_xref="CDD:119332"
     Site            order(279,308,362,432..433,487,511,538,540,588,590)
                     /site_type="active"
                     /db_xref="CDD:119332"
     CDS             1..661
                     /gene="fdl"
                     /coded_by="XM_034324514.2:141..2126"
                     /db_xref="GeneID:117604439"
ORIGIN      
        1 mpeafirrsl fhrgtyfhdf daflssyver larmvgsvps gwmrkillfl vlmtgvllia
       61 myahapplvs lqpfssrrtf qspwswacva grcerravrs srtslascia lcggntrllw
      121 prptgnvvlg ddsviihlrq iefvtvntsd qetknlleha kdifignirs lvkvansksr
      181 pgvdtfliyl sagngravgp nldtdesytl elipkgrtle aringksyfg vrhgletlgq
      241 miwwdetagr egalrvlsha tvedkpmfgy rgllvdtgrq ffsierlkrv idgmaaskln
      301 tfhwhltdsq sfpfdsaqfp emarwgaysg dqiytpddvk dladyarirg irvlveidsp
      361 ahagagwqwg teygygelal cvdqqpwssy cgepncgqln pinehsyril eglyrelldl
      421 teirdivhlg gdevnldcwa qygnitaamq aqnmtdhham waefetkimq rlvkanhdqt
      481 pkavilwssp ltkrpyiimy fdpkihviqs wggstwpetp dlledgfrvi lshvdawyld
      541 cgfgrwretg eaacgeyrtw qtvynhrpwr dypqqhlhlv lggeaaiwse qtgdaslgpr
      601 lwprasalae rlwsdlptng ystdesvytr laahmevlts rglkteamwp qwcsqnpgkc
      661 l
//
LOCUS       XP_034180416             635 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604454 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180416
VERSION     XP_034180416.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324525.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..635
                     /product="uncharacterized protein LOC117604454 isoform X1"
                     /calculated_mol_wt=71269
     Region          49..297
                     /region_name="Tmemb_40"
                     /note="Predicted membrane protein; pfam10160"
                     /db_xref="CDD:462970"
     CDS             1..635
                     /gene="LOC117604454"
                     /coded_by="XM_034324525.2:194..2101"
                     /db_xref="GeneID:117604454"
ORIGIN      
        1 mygavreevs eswlhtydls stmipnvssp itdeehfckf ilykeikdsr vriwdivili
       61 pnllfllfia irfnrarlkl ratsspiffa fyglvicniv isvircvvsm tvnaaatvgg
      121 kadkvlwvtv rffllstems vlifglafgh ldsrssirrv llatsliala ftitqgtlel
      181 vlpddtfrip srnlyvfghg gmmfwfcssl vftmiylfil ilpwtrlrdr ltlptrksfy
      241 iyagtlamld lvqsigagfl nytqnpvglc ivdftaivyl tlftplvyht flseffgvsq
      301 psimfsykaq vddamdedtv slphqqsfss lktdsdyiyq vhtpsihmpl ydsqalkssp
      361 tkhktslhsl aspkdfkvas ghaydsrvtl yrsppgiksf arrlsaekta fdfknypltk
      421 sdnfidskts apdlrfpnta wksrstsqfk yggpssvsnl lfspstasnf lyksgandss
      481 tnlfsltrks sleherkgse gsvpaqffei ppletttlgq epltkdkavs riegdrkqls
      541 plkqyantgm sevqvedasd smdnsnatqq llfksitltr edtfkrrkre pkkresagls
      601 dyllslttpk pvkdsevdps ppsdhtksyt qtesl
//
LOCUS       XP_034180423             635 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604454 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180423
VERSION     XP_034180423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..635
                     /product="uncharacterized protein LOC117604454 isoform X1"
                     /calculated_mol_wt=71269
     Region          49..297
                     /region_name="Tmemb_40"
                     /note="Predicted membrane protein; pfam10160"
                     /db_xref="CDD:462970"
     CDS             1..635
                     /gene="LOC117604454"
                     /coded_by="XM_034324532.2:192..2099"
                     /db_xref="GeneID:117604454"
ORIGIN      
        1 mygavreevs eswlhtydls stmipnvssp itdeehfckf ilykeikdsr vriwdivili
       61 pnllfllfia irfnrarlkl ratsspiffa fyglvicniv isvircvvsm tvnaaatvgg
      121 kadkvlwvtv rffllstems vlifglafgh ldsrssirrv llatsliala ftitqgtlel
      181 vlpddtfrip srnlyvfghg gmmfwfcssl vftmiylfil ilpwtrlrdr ltlptrksfy
      241 iyagtlamld lvqsigagfl nytqnpvglc ivdftaivyl tlftplvyht flseffgvsq
      301 psimfsykaq vddamdedtv slphqqsfss lktdsdyiyq vhtpsihmpl ydsqalkssp
      361 tkhktslhsl aspkdfkvas ghaydsrvtl yrsppgiksf arrlsaekta fdfknypltk
      421 sdnfidskts apdlrfpnta wksrstsqfk yggpssvsnl lfspstasnf lyksgandss
      481 tnlfsltrks sleherkgse gsvpaqffei ppletttlgq epltkdkavs riegdrkqls
      541 plkqyantgm sevqvedasd smdnsnatqq llfksitltr edtfkrrkre pkkresagls
      601 dyllslttpk pvkdsevdps ppsdhtksyt qtesl
//
LOCUS       XP_034180432             390 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein adipocyte-associated 1 homolog isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034180432
VERSION     XP_034180432.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324541.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="transmembrane protein adipocyte-associated 1
                     homolog isoform X2"
                     /calculated_mol_wt=43955
     Region          49..297
                     /region_name="Tmemb_40"
                     /note="Predicted membrane protein; pfam10160"
                     /db_xref="CDD:462970"
     CDS             1..390
                     /gene="LOC117604454"
                     /coded_by="XM_034324541.2:194..1366"
                     /db_xref="GeneID:117604454"
ORIGIN      
        1 mygavreevs eswlhtydls stmipnvssp itdeehfckf ilykeikdsr vriwdivili
       61 pnllfllfia irfnrarlkl ratsspiffa fyglvicniv isvircvvsm tvnaaatvgg
      121 kadkvlwvtv rffllstems vlifglafgh ldsrssirrv llatsliala ftitqgtlel
      181 vlpddtfrip srnlyvfghg gmmfwfcssl vftmiylfil ilpwtrlrdr ltlptrksfy
      241 iyagtlamld lvqsigagfl nytqnpvglc ivdftaivyl tlftplvyht flseffgvsq
      301 psimfsykaq vddamdedtv slphqqsfss lktdsdyiyq nhsvydstqf dtnstpvnpl
      361 yaaslqspds itgysidsqe gqtqangyqq
//
LOCUS       XP_034180444             569 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604466 [Osmia lignaria lignaria].
ACCESSION   XP_034180444
VERSION     XP_034180444.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324553.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180444.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..569
                     /product="uncharacterized protein LOC117604466"
                     /calculated_mol_wt=64417
     Region          40..124
                     /region_name="MANEC"
                     /note="MANEC domain; pfam07502"
                     /db_xref="CDD:462186"
     Region          194..226
                     /region_name="LDLa"
                     /note="Low-density lipoprotein receptor domain class A;
                     smart00192"
                     /db_xref="CDD:197566"
     Site            order(199,208,219..220)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(212,215,219,225..226)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            222..226
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..569
                     /gene="LOC117604466"
                     /coded_by="XM_034324553.2:37..1746"
                     /db_xref="GeneID:117604466"
ORIGIN      
        1 mlssnviqic inlftilycv ageillnkne krasfrnnyq mcfesfnvle nkiirtqesq
       61 dmgakylkem dvnsrseclr fccdtkncdv fifeekkpgs cylfqcgpsh dfkckftnha
      121 nyssaihtny nsqgnaqleq eirisqqehe lkslrklgni apaeysftep pikliatlap
      181 klsltttppi kqgcrpnqyk crssgeciav ynvcdgipqc adesdeaade cptekptisp
      241 iiqgpqpppp plpidnikyq qmvdqqkfpv pqyahpevns wmlpnsdqqm iprpisypaq
      301 qmeilpihkg ysapgyqwnl qpiydqnkdi ynpinsfheq snlnryehqp hsfnhkgssv
      361 igeketdgga ylderhpyis hfgsnrgpwq ntqifpspsp ipnlppkqid eiennvastv
      421 appcdisneq ryiskskevk kttqqgekhv ahekeidqns akentlkmet pklnhangke
      481 pkehksvivi kdhsrehgrn tiiadhlkqp neievlkprg avislalglt itaitatlia
      541 crlrvvrrrg rrhgpyahda dylvngmyl
//
LOCUS       XP_034180457             500 aa            linear   INV 03-SEP-2025
DEFINITION  secretin receptor [Osmia lignaria lignaria].
ACCESSION   XP_034180457
VERSION     XP_034180457.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324566.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..500
                     /product="secretin receptor"
                     /calculated_mol_wt=58131
     Region          44..110
                     /region_name="HRM"
                     /note="Hormone receptor domain; pfam02793"
                     /db_xref="CDD:397086"
     Region          149..433
                     /region_name="7tmB1_NPR_B7_insect-like"
                     /note="insect neuropeptide receptor subgroup B7 and
                     related proteins, member of the class B family of
                     seven-transmembrane G protein-coupled receptors; cd15273"
                     /db_xref="CDD:320401"
     Region          151..176
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320401"
     Site            order(156,160,202,205..206,209,240,247,318..319,321,323,
                     379,382,398,402)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:320401"
     Region          185..207
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320401"
     Region          240..267
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320401"
     Region          279..299
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320401"
     Region          315..344
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320401"
     Region          360..387
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320401"
     Region          395..420
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320401"
     CDS             1..500
                     /gene="LOC117604474"
                     /coded_by="XM_034324566.2:1602..3104"
                     /db_xref="GeneID:117604474"
ORIGIN      
        1 mtttetdvts etegffveqk rkcgelmney lnvtlengsp rfhhcppsfd gllcwpytka
       61 sttailpcpp esdfdsnnra famasklcla ngrwykntee ipwsnyslce lsdeftaryl
      121 metiadlgig mtrsrdygnf esflldkwlp ivrivsqigy atsftmliia miifsllrkl
      181 rnprnrlhmh lfasfimraf malirdwifv dgvglavdvv yvdeknafik qrnfqtlick
      241 titsawqffi vanyswilme glylhnlvvl afcadsaain lyilmgwglp vfvvvpwiii
      301 ratiedtlcw tthdnpsvfl liripimist lfnfllfini vrvlfikfkt svhlqrkkmq
      361 ysrwakstlv lvplfgahyt lflglsyhkd hrvelvwlfc dqffasfqgs fvallyclln
      421 ievrteikra wqarwsknvn ifvsncrepr krmsnrhcrq sndyeqptvs gttmrnlftl
      481 qserrsnndy wpnvlemetg
//
LOCUS       XP_034180470             463 aa            linear   INV 03-SEP-2025
DEFINITION  Golgi resident protein GCP60 [Osmia lignaria lignaria].
ACCESSION   XP_034180470
VERSION     XP_034180470.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324579.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..463
                     /product="Golgi resident protein GCP60"
                     /calculated_mol_wt=53750
     Region          49..129
                     /region_name="ACBP"
                     /note="Acyl CoA binding protein; pfam00887"
                     /db_xref="CDD:459982"
     Region          318..458
                     /region_name="GPCR_chapero_1"
                     /note="GPCR-chaperone; cl46312"
                     /db_xref="CDD:480652"
     CDS             1..463
                     /gene="LOC117604482"
                     /coded_by="XM_034324579.2:141..1532"
                     /db_xref="GeneID:117604482"
ORIGIN      
        1 magtngdisl vgkkiekltv skeestkden dkekkkkses hfwgfetrel ykiainfyke
       61 kegkavhlsy edklklvaft qqvthgkcta enapplgvld vigkdrrlaw hnlgdiskeq
      121 amegfivlld klcplfrtvv eaqrrdneek lrlkkeeear kleeerrlke ldeqkkkeee
      181 arlkeeiqrr qiqdalnqqt yyqfkvyaeq qypgnpeqqg vlirqlqeqh yhqymqqlrq
      241 nqlviedqsp dtnkvtaeie etkehketse taplndedsd elpedfppiv paemwtrkgv
      301 eefketirre agdavikvgh geivtvrvpt hedgsclfwe fatdgydigf gvyfewskpe
      361 tnqvsvhise sededeeddd ydskedlesg vstgtiqsdy kplpppisvi vpvyrrdsqe
      421 eiyagshryp gqgvyllkfd nsyslwrskt lyyrvyytqn gvv
//
LOCUS       XP_034180478             463 aa            linear   INV 03-SEP-2025
DEFINITION  Golgi resident protein GCP60 [Osmia lignaria lignaria].
ACCESSION   XP_034180478
VERSION     XP_034180478.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324587.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..463
                     /product="Golgi resident protein GCP60"
                     /calculated_mol_wt=53750
     Region          49..129
                     /region_name="ACBP"
                     /note="Acyl CoA binding protein; pfam00887"
                     /db_xref="CDD:459982"
     Region          318..458
                     /region_name="GPCR_chapero_1"
                     /note="GPCR-chaperone; cl46312"
                     /db_xref="CDD:480652"
     CDS             1..463
                     /gene="LOC117604482"
                     /coded_by="XM_034324587.2:270..1661"
                     /db_xref="GeneID:117604482"
ORIGIN      
        1 magtngdisl vgkkiekltv skeestkden dkekkkkses hfwgfetrel ykiainfyke
       61 kegkavhlsy edklklvaft qqvthgkcta enapplgvld vigkdrrlaw hnlgdiskeq
      121 amegfivlld klcplfrtvv eaqrrdneek lrlkkeeear kleeerrlke ldeqkkkeee
      181 arlkeeiqrr qiqdalnqqt yyqfkvyaeq qypgnpeqqg vlirqlqeqh yhqymqqlrq
      241 nqlviedqsp dtnkvtaeie etkehketse taplndedsd elpedfppiv paemwtrkgv
      301 eefketirre agdavikvgh geivtvrvpt hedgsclfwe fatdgydigf gvyfewskpe
      361 tnqvsvhise sededeeddd ydskedlesg vstgtiqsdy kplpppisvi vpvyrrdsqe
      421 eiyagshryp gqgvyllkfd nsyslwrskt lyyrvyytqn gvv
//
LOCUS       XP_034180489             459 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 145 [Osmia lignaria lignaria].
ACCESSION   XP_034180489
VERSION     XP_034180489.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324598.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180489.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="transmembrane protein 145"
                     /calculated_mol_wt=52410
     Region          146..402
                     /region_name="Lung_7-TM_R"
                     /note="Lung seven transmembrane receptor; cl21735"
                     /db_xref="CDD:473945"
     CDS             1..459
                     /gene="LOC117604492"
                     /coded_by="XM_034324598.2:22..1401"
                     /db_xref="GeneID:117604492"
ORIGIN      
        1 mvnvsfffci illflltnse skilqghlvt renwaflarf cflteqgtfr yefelggdeq
       61 nlklllyyda pdqwpsvyps nktciekeal lwdgigqivp lslstseqsg cvqedtitrc
      121 ssyrrfrssr prwwfialad cssesglnvs ywisltnaph gnfwkehfsa defyilpeli
      181 iiaciyivlv ilsfyaamql rarrllhvsy klfmasllcq lvgilfeiys yinlglrgts
      241 tnnasllgql leacsdilyt llmlllalgf tvtksvlkpk qirwlicfic lvsfcqlsly
      301 iyqsdvfdpg lvlyiyespp gygliilkli awivfsvccf ktvrkmstkl hfygslfslg
      361 stwflchplt vlcitllvde wiresvakgc slwivflghi iflyitrpsm ankrfpfhir
      421 tcqvmpiagd gqdhsyeprv rttgsaftis hlppptlqs
//
LOCUS       XP_034174046             457 aa            linear   INV 03-SEP-2025
DEFINITION  juvenile hormone epoxide hydrolase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034174046
VERSION     XP_034174046.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318155.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="juvenile hormone epoxide hydrolase 1"
                     /calculated_mol_wt=51570
     Region          47..159
                     /region_name="EHN"
                     /note="Epoxide hydrolase N terminus; pfam06441"
                     /db_xref="CDD:461913"
     Region          150..>247
                     /region_name="MenH"
                     /note="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase MenH and related
                     esterases, alpha/beta hydrolase fold [Coenzyme transport
                     and metabolism, General function prediction only];
                     COG0596"
                     /db_xref="CDD:440361"
     CDS             1..457
                     /gene="LOC117601433"
                     /coded_by="XM_034318155.2:139..1512"
                     /db_xref="GeneID:117601433"
ORIGIN      
        1 mwksivfltl aaigigsyln fptehhvptl pepnwgpkka akasteirpf kidvpksvqe
       61 dlknrlenrk tyvepledaa wtygistkyl ntvldywknk ynwterqall nkypqfktii
      121 qgldihfyhv kptnlpkdkn lkvlpllllh gwpgsvvefq kiipmltkpw pnhdfvfevi
      181 apslpgygfs qgaarpglgt aqmavvfknl mqrlgfnkfy lqggdwgsli asnmaalype
      241 kvtgvhlnmc tglalknwfw llvgtyfpsl vggsehysly ypvskhfttl meesgyfhiq
      301 atkpdtvgvg lssapdglaa yilekfstwt nkeyrsrddg gllekftlde lldnvmvywi
      361 tnsittsarl yaenfsspnr alkidelpik vptacavfps ellalpqsll tgrypniiqh
      421 nvlprgghfa afeeprllad difsfvkkne elkkkss
//
LOCUS       XP_034180514             448 aa            linear   INV 03-SEP-2025
DEFINITION  reticulon isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180514
VERSION     XP_034180514.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324623.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180514.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..448
                     /product="reticulon isoform X1"
                     /calculated_mol_wt=50400
     Region          <38..>193
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          258..422
                     /region_name="Reticulon"
                     /note="pfam02453"
                     /db_xref="CDD:460562"
     CDS             1..448
                     /gene="Rtnl1"
                     /coded_by="XM_034324623.2:422..1768"
                     /db_xref="GeneID:117604507"
ORIGIN      
        1 maelkeqpir rehdsmddfe hlehvqdgqs shlephtekv qenqehslnm ekdminfspn
       61 qtvnepedpr katllllese kgkpaaednf evrpkepehd llmdkiedhs evmnvepisk
      121 kdeekqkpve kaeikdkkiv dhepavvsst ekkaddawnv ieksqpives iskepkkdts
      181 aseyvlaetg kkeqisapvs rklefesele espaksfksv kqdsrterie eveiapkeif
      241 ssmgidawfn pdrlnpkvaa liywrdpkks gavfgcilgv llslayfsli svlaylsllv
      301 ltgtiafriy ktvlqalqkt sdghpfkdil eldltlsaek vhevtvvava hanatvselr
      361 rlflvedfvd slkfgvllwc ltyvgswfng mtliiigvia lftlpkvyet nkaqidqnla
      421 lvhgkinelt akvkaaiplg kkeptkee
//
LOCUS       XP_034180523             440 aa            linear   INV 03-SEP-2025
DEFINITION  reticulon isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034180523
VERSION     XP_034180523.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324632.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180523.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..440
                     /product="reticulon isoform X2"
                     /calculated_mol_wt=49422
     Region          <38..>193
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          251..414
                     /region_name="Reticulon"
                     /note="pfam02453"
                     /db_xref="CDD:460562"
     CDS             1..440
                     /gene="Rtnl1"
                     /coded_by="XM_034324632.2:422..1744"
                     /db_xref="GeneID:117604507"
ORIGIN      
        1 maelkeqpir rehdsmddfe hlehvqdgqs shlephtekv qenqehslnm ekdminfspn
       61 qtvnepedpr katllllese kgkpaaednf evrpkepehd llmdkiedhs evmnvepisk
      121 kdeekqkpve kaeikdkkiv dhepavvsst ekkaddawnv ieksqpives iskepkkdts
      181 aseyvlaetg kkeqisapvs rklefesele espaksfksv kqdsrterie eveiapkeif
      241 ssmgigeyil aaliywrdpk ksgavfgcil gvllslayfs lisvlaylsl lvltgtiafr
      301 iyktvlqalq ktsdghpfkd ileldltlsa ekvhevtvva vahanatvse lrrlflvedf
      361 vdslkfgvll wcltyvgswf ngmtliiigv ialftlpkvy etnkaqidqn lalvhgkine
      421 ltakvkaaip lgkkeptkee
//
LOCUS       XP_034180535             436 aa            linear   INV 03-SEP-2025
DEFINITION  reticulon isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034180535
VERSION     XP_034180535.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324644.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180535.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..436
                     /product="reticulon isoform X3"
                     /calculated_mol_wt=48945
     Region          <3..246
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          246..410
                     /region_name="Reticulon"
                     /note="pfam02453"
                     /db_xref="CDD:460562"
     CDS             1..436
                     /gene="Rtnl1"
                     /coded_by="XM_034324644.2:422..1732"
                     /db_xref="GeneID:117604507"
ORIGIN      
        1 maelkeqpir rehdsmddfe hlehvqdgqs shlephtekv qenqehslnm ekdminfspn
       61 qtvnepedpr katllllese kgkpaaednf evrpkepehd llmdkiedhs evmnvepisk
      121 kdeekqkpve kaeikdkkiv dhepavvsst ekkaddawnv ieksqpives iskepkkdts
      181 aseyvlaetg kkeqisapvs rklefesele espaksfksv kqdsrterie eveiapkeif
      241 ssmgivaali ywrdpkksga vfgcilgvll slayfslisv laylsllvlt gtiafriykt
      301 vlqalqktsd ghpfkdilel dltlsaekvh evtvvavaha natvselrrl flvedfvdsl
      361 kfgvllwclt yvgswfngmt liiigvialf tlpkvyetnk aqidqnlalv hgkineltak
      421 vkaaiplgkk eptkee
//
LOCUS       XP_034180545             246 aa            linear   INV 03-SEP-2025
DEFINITION  reticulon isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034180545
VERSION     XP_034180545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324654.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..246
                     /product="reticulon isoform X4"
                     /calculated_mol_wt=26963
     Region          56..220
                     /region_name="Reticulon"
                     /note="pfam02453"
                     /db_xref="CDD:460562"
     CDS             1..246
                     /gene="Rtnl1"
                     /coded_by="XM_034324654.2:318..1058"
                     /db_xref="GeneID:117604507"
ORIGIN      
        1 metepvcpin lpakekddag avckkpcssv angspcfwni ldpyawfnpd rlnpkvaali
       61 ywrdpkksga vfgcilgvll slayfslisv laylsllvlt gtiafriykt vlqalqktsd
      121 ghpfkdilel dltlsaekvh evtvvavaha natvselrrl flvedfvdsl kfgvllwclt
      181 yvgswfngmt liiigvialf tlpkvyetnk aqidqnlalv hgkineltak vkaaiplgkk
      241 eptkee
//
LOCUS       XP_034180554             234 aa            linear   INV 03-SEP-2025
DEFINITION  reticulon isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034180554
VERSION     XP_034180554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324663.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..234
                     /product="reticulon isoform X5"
                     /calculated_mol_wt=25474
     Region          45..208
                     /region_name="Reticulon"
                     /note="pfam02453"
                     /db_xref="CDD:460562"
     CDS             1..234
                     /gene="Rtnl1"
                     /coded_by="XM_034324663.2:318..1022"
                     /db_xref="GeneID:117604507"
ORIGIN      
        1 metepvcpin lpakekddag avckkpcssv angspcfwni ldplaaliyw rdpkksgavf
       61 gcilgvllsl ayfslisvla ylsllvltgt iafriyktvl qalqktsdgh pfkdileldl
      121 tlsaekvhev tvvavahana tvselrrlfl vedfvdslkf gvllwcltyv gswfngmtli
      181 iigvialftl pkvyetnkaq idqnlalvhg kineltakvk aaiplgkkep tkee
//
LOCUS       XP_034180565             210 aa            linear   INV 03-SEP-2025
DEFINITION  reticulon isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034180565
VERSION     XP_034180565.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324674.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..210
                     /product="reticulon isoform X6"
                     /calculated_mol_wt=23121
     Region          20..184
                     /region_name="Reticulon"
                     /note="pfam02453"
                     /db_xref="CDD:460562"
     CDS             1..210
                     /gene="Rtnl1"
                     /coded_by="XM_034324674.2:363..995"
                     /db_xref="GeneID:117604507"
ORIGIN      
        1 mvrrnnlrap medlpdrgpv aaliywrdpk ksgavfgcil gvllslayfs lisvlaylsl
       61 lvltgtiafr iyktvlqalq ktsdghpfkd ileldltlsa ekvhevtvva vahanatvse
      121 lrrlflvedf vdslkfgvll wcltyvgswf ngmtliiigv ialftlpkvy etnkaqidqn
      181 lalvhgkine ltakvkaaip lgkkeptkee
//
LOCUS       XP_034180575             198 aa            linear   INV 03-SEP-2025
DEFINITION  reticulon isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034180575
VERSION     XP_034180575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..198
                     /product="reticulon isoform X7"
                     /calculated_mol_wt=21761
     Region          9..172
                     /region_name="Reticulon"
                     /note="pfam02453"
                     /db_xref="CDD:460562"
     CDS             1..198
                     /gene="Rtnl1"
                     /coded_by="XM_034324684.2:141..737"
                     /db_xref="GeneID:117604507"
ORIGIN      
        1 maidreelaa liywrdpkks gavfgcilgv llslayfsli svlaylsllv ltgtiafriy
       61 ktvlqalqkt sdghpfkdil eldltlsaek vhevtvvava hanatvselr rlflvedfvd
      121 slkfgvllwc ltyvgswfng mtliiigvia lftlpkvyet nkaqidqnla lvhgkinelt
      181 akvkaaiplg kkeptkee
//
LOCUS       XP_034174056             447 aa            linear   INV 03-SEP-2025
DEFINITION  guanine nucleotide exchange factor for Rab-3A [Osmia lignaria
            lignaria].
ACCESSION   XP_034174056
VERSION     XP_034174056.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318165.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..447
                     /product="guanine nucleotide exchange factor for Rab-3A"
                     /calculated_mol_wt=51568
     Region          129..>180
                     /region_name="Sec2p"
                     /note="GDP/GTP exchange factor Sec2p; pfam06428"
                     /db_xref="CDD:428938"
     Region          292..442
                     /region_name="Rab11BD_RAB3IP_like"
                     /note="Rab11 binding domain of Rab-3A-interacting protein
                     (RAB3IP), Rab-3A-interacting-like protein 1 (RAB3IL1) and
                     similar proteins; cd21044"
                     /db_xref="CDD:411031"
     Site            order(349..354,401,404..405,408,412,417..419)
                     /site_type="other"
                     /note="Rab11 binding site [polypeptide binding]"
                     /db_xref="CDD:411031"
     CDS             1..447
                     /gene="LOC117601442"
                     /coded_by="XM_034318165.2:229..1572"
                     /db_xref="GeneID:117601442"
ORIGIN      
        1 meerkltksc dtlarlnene hklfenrnhc tkplpiifne engedlindq dcvddstrin
       61 ccnnsskall clkdseksqe keepcslkki nqqppspttl qwgraiaevk ehavaklqde
      121 lkraheelkl kdeevtrlsr ikqdveaele eltaslfqea hnmvreanvr qataerllee
      181 srmkaevlaa evaalktlvl tstpakpnfh lhpqidtksr snsedgqqgl ftrkhrrsps
      241 hfnlkygren sppdsplkeq rpslpsdres lerdwkkdsd kekdkeckdf glevdpriht
      301 eflkwkanpc vdkgdpfvgr vfkedidlcl dfpnkelgtk vrqavldgii fieaisdktk
      361 ftfpkkcvll eaprqcyyrm rlgdqenqwy sisqicrnri iavcdflnyl ryierglvks
      421 svhdvyweit rlrkemafar lglalss
//
LOCUS       XP_034174065             447 aa            linear   INV 03-SEP-2025
DEFINITION  guanine nucleotide exchange factor for Rab-3A [Osmia lignaria
            lignaria].
ACCESSION   XP_034174065
VERSION     XP_034174065.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318174.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..447
                     /product="guanine nucleotide exchange factor for Rab-3A"
                     /calculated_mol_wt=51568
     Region          129..>180
                     /region_name="Sec2p"
                     /note="GDP/GTP exchange factor Sec2p; pfam06428"
                     /db_xref="CDD:428938"
     Region          292..442
                     /region_name="Rab11BD_RAB3IP_like"
                     /note="Rab11 binding domain of Rab-3A-interacting protein
                     (RAB3IP), Rab-3A-interacting-like protein 1 (RAB3IL1) and
                     similar proteins; cd21044"
                     /db_xref="CDD:411031"
     Site            order(349..354,401,404..405,408,412,417..419)
                     /site_type="other"
                     /note="Rab11 binding site [polypeptide binding]"
                     /db_xref="CDD:411031"
     CDS             1..447
                     /gene="LOC117601442"
                     /coded_by="XM_034318174.2:227..1570"
                     /db_xref="GeneID:117601442"
ORIGIN      
        1 meerkltksc dtlarlnene hklfenrnhc tkplpiifne engedlindq dcvddstrin
       61 ccnnsskall clkdseksqe keepcslkki nqqppspttl qwgraiaevk ehavaklqde
      121 lkraheelkl kdeevtrlsr ikqdveaele eltaslfqea hnmvreanvr qataerllee
      181 srmkaevlaa evaalktlvl tstpakpnfh lhpqidtksr snsedgqqgl ftrkhrrsps
      241 hfnlkygren sppdsplkeq rpslpsdres lerdwkkdsd kekdkeckdf glevdpriht
      301 eflkwkanpc vdkgdpfvgr vfkedidlcl dfpnkelgtk vrqavldgii fieaisdktk
      361 ftfpkkcvll eaprqcyyrm rlgdqenqwy sisqicrnri iavcdflnyl ryierglvks
      421 svhdvyweit rlrkemafar lglalss
//
LOCUS       XP_034174073             447 aa            linear   INV 03-SEP-2025
DEFINITION  guanine nucleotide exchange factor for Rab-3A [Osmia lignaria
            lignaria].
ACCESSION   XP_034174073
VERSION     XP_034174073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..447
                     /product="guanine nucleotide exchange factor for Rab-3A"
                     /calculated_mol_wt=51568
     Region          129..>180
                     /region_name="Sec2p"
                     /note="GDP/GTP exchange factor Sec2p; pfam06428"
                     /db_xref="CDD:428938"
     Region          292..442
                     /region_name="Rab11BD_RAB3IP_like"
                     /note="Rab11 binding domain of Rab-3A-interacting protein
                     (RAB3IP), Rab-3A-interacting-like protein 1 (RAB3IL1) and
                     similar proteins; cd21044"
                     /db_xref="CDD:411031"
     Site            order(349..354,401,404..405,408,412,417..419)
                     /site_type="other"
                     /note="Rab11 binding site [polypeptide binding]"
                     /db_xref="CDD:411031"
     CDS             1..447
                     /gene="LOC117601442"
                     /coded_by="XM_034318182.2:274..1617"
                     /db_xref="GeneID:117601442"
ORIGIN      
        1 meerkltksc dtlarlnene hklfenrnhc tkplpiifne engedlindq dcvddstrin
       61 ccnnsskall clkdseksqe keepcslkki nqqppspttl qwgraiaevk ehavaklqde
      121 lkraheelkl kdeevtrlsr ikqdveaele eltaslfqea hnmvreanvr qataerllee
      181 srmkaevlaa evaalktlvl tstpakpnfh lhpqidtksr snsedgqqgl ftrkhrrsps
      241 hfnlkygren sppdsplkeq rpslpsdres lerdwkkdsd kekdkeckdf glevdpriht
      301 eflkwkanpc vdkgdpfvgr vfkedidlcl dfpnkelgtk vrqavldgii fieaisdktk
      361 ftfpkkcvll eaprqcyyrm rlgdqenqwy sisqicrnri iavcdflnyl ryierglvks
      421 svhdvyweit rlrkemafar lglalss
//
LOCUS       XP_034174081             447 aa            linear   INV 03-SEP-2025
DEFINITION  guanine nucleotide exchange factor for Rab-3A [Osmia lignaria
            lignaria].
ACCESSION   XP_034174081
VERSION     XP_034174081.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318190.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..447
                     /product="guanine nucleotide exchange factor for Rab-3A"
                     /calculated_mol_wt=51568
     Region          129..>180
                     /region_name="Sec2p"
                     /note="GDP/GTP exchange factor Sec2p; pfam06428"
                     /db_xref="CDD:428938"
     Region          292..442
                     /region_name="Rab11BD_RAB3IP_like"
                     /note="Rab11 binding domain of Rab-3A-interacting protein
                     (RAB3IP), Rab-3A-interacting-like protein 1 (RAB3IL1) and
                     similar proteins; cd21044"
                     /db_xref="CDD:411031"
     Site            order(349..354,401,404..405,408,412,417..419)
                     /site_type="other"
                     /note="Rab11 binding site [polypeptide binding]"
                     /db_xref="CDD:411031"
     CDS             1..447
                     /gene="LOC117601442"
                     /coded_by="XM_034318190.2:256..1599"
                     /db_xref="GeneID:117601442"
ORIGIN      
        1 meerkltksc dtlarlnene hklfenrnhc tkplpiifne engedlindq dcvddstrin
       61 ccnnsskall clkdseksqe keepcslkki nqqppspttl qwgraiaevk ehavaklqde
      121 lkraheelkl kdeevtrlsr ikqdveaele eltaslfqea hnmvreanvr qataerllee
      181 srmkaevlaa evaalktlvl tstpakpnfh lhpqidtksr snsedgqqgl ftrkhrrsps
      241 hfnlkygren sppdsplkeq rpslpsdres lerdwkkdsd kekdkeckdf glevdpriht
      301 eflkwkanpc vdkgdpfvgr vfkedidlcl dfpnkelgtk vrqavldgii fieaisdktk
      361 ftfpkkcvll eaprqcyyrm rlgdqenqwy sisqicrnri iavcdflnyl ryierglvks
      421 svhdvyweit rlrkemafar lglalss
//
LOCUS       XP_034180589             427 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180589
VERSION     XP_034180589.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324698.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="retinoid X receptor ultraspiracle isoform X1"
                     /calculated_mol_wt=47820
     Region          108..184
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(110,113,127,130,146,152,162,165)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(115,156..157,159,161)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(119..122,128..129,131,133,135..136,139,147,159..160,
                     163,166,179..181,183..184)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(141,164,167..168,171..172,176..178,182..184)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(157..158,161)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            178..183
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(182,184)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          204..406
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(234..235,237..238,241,275,279,282,292..293,308,398,
                     401..402)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(243,246,250,255,260..261,263..264,268)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,339,345,359..360,363..364,367,381..382,
                     385..386,388..390,392..393,396,400)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,345,347,356,363,367,379,382..383,
                     386..389,392..393,396,400)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..427
                     /gene="usp"
                     /coded_by="XM_034324698.2:396..1679"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mmkkerpmts vtaiiqgtqa qhwsrgntwl sldnsnmsms svgpqspldm kpdtaslina
       61 gnfspsgpns pgsfnagchs nllstspsgq nkavapyppn hplsgskhlc sicgdrasgk
      121 hygvyscegc kgffkrtvrk dlsyacreek sciidkrqrn rcqycryqkc lamgmkreav
      181 qeerqrtker dqsevestss lhsdmpieri leaekrveck meqqgnyena vsqicnatnk
      241 qlfqlvawak hiphftslpl edqvlllrag wnelliasfs hrsidvkdgi vlatgitvhr
      301 nsaqqagvgt ifdrvlselv skmremkmdr telgclrsii lfnpevrglk siqevtllre
      361 kiyaalegyc rvawpddpgr faklllrlpa irsiglkcle hlfffkmigd vpiddflvem
      421 lesrsdp
//
LOCUS       XP_034180599             427 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180599
VERSION     XP_034180599.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324708.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="retinoid X receptor ultraspiracle isoform X1"
                     /calculated_mol_wt=47820
     Region          108..184
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(110,113,127,130,146,152,162,165)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(115,156..157,159,161)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(119..122,128..129,131,133,135..136,139,147,159..160,
                     163,166,179..181,183..184)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(141,164,167..168,171..172,176..178,182..184)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(157..158,161)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            178..183
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(182,184)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          204..406
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(234..235,237..238,241,275,279,282,292..293,308,398,
                     401..402)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(243,246,250,255,260..261,263..264,268)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,339,345,359..360,363..364,367,381..382,
                     385..386,388..390,392..393,396,400)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,345,347,356,363,367,379,382..383,
                     386..389,392..393,396,400)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..427
                     /gene="usp"
                     /coded_by="XM_034324708.2:437..1720"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mmkkerpmts vtaiiqgtqa qhwsrgntwl sldnsnmsms svgpqspldm kpdtaslina
       61 gnfspsgpns pgsfnagchs nllstspsgq nkavapyppn hplsgskhlc sicgdrasgk
      121 hygvyscegc kgffkrtvrk dlsyacreek sciidkrqrn rcqycryqkc lamgmkreav
      181 qeerqrtker dqsevestss lhsdmpieri leaekrveck meqqgnyena vsqicnatnk
      241 qlfqlvawak hiphftslpl edqvlllrag wnelliasfs hrsidvkdgi vlatgitvhr
      301 nsaqqagvgt ifdrvlselv skmremkmdr telgclrsii lfnpevrglk siqevtllre
      361 kiyaalegyc rvawpddpgr faklllrlpa irsiglkcle hlfffkmigd vpiddflvem
      421 lesrsdp
//
LOCUS       XP_034180608             427 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180608
VERSION     XP_034180608.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324717.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="retinoid X receptor ultraspiracle isoform X1"
                     /calculated_mol_wt=47820
     Region          108..184
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(110,113,127,130,146,152,162,165)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(115,156..157,159,161)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(119..122,128..129,131,133,135..136,139,147,159..160,
                     163,166,179..181,183..184)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(141,164,167..168,171..172,176..178,182..184)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(157..158,161)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            178..183
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(182,184)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          204..406
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(234..235,237..238,241,275,279,282,292..293,308,398,
                     401..402)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(243,246,250,255,260..261,263..264,268)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,339,345,359..360,363..364,367,381..382,
                     385..386,388..390,392..393,396,400)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,345,347,356,363,367,379,382..383,
                     386..389,392..393,396,400)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..427
                     /gene="usp"
                     /coded_by="XM_034324717.2:1201..2484"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mmkkerpmts vtaiiqgtqa qhwsrgntwl sldnsnmsms svgpqspldm kpdtaslina
       61 gnfspsgpns pgsfnagchs nllstspsgq nkavapyppn hplsgskhlc sicgdrasgk
      121 hygvyscegc kgffkrtvrk dlsyacreek sciidkrqrn rcqycryqkc lamgmkreav
      181 qeerqrtker dqsevestss lhsdmpieri leaekrveck meqqgnyena vsqicnatnk
      241 qlfqlvawak hiphftslpl edqvlllrag wnelliasfs hrsidvkdgi vlatgitvhr
      301 nsaqqagvgt ifdrvlselv skmremkmdr telgclrsii lfnpevrglk siqevtllre
      361 kiyaalegyc rvawpddpgr faklllrlpa irsiglkcle hlfffkmigd vpiddflvem
      421 lesrsdp
//
LOCUS       XP_034180617             427 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180617
VERSION     XP_034180617.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324726.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="retinoid X receptor ultraspiracle isoform X1"
                     /calculated_mol_wt=47820
     Region          108..184
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(110,113,127,130,146,152,162,165)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(115,156..157,159,161)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(119..122,128..129,131,133,135..136,139,147,159..160,
                     163,166,179..181,183..184)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(141,164,167..168,171..172,176..178,182..184)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(157..158,161)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            178..183
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(182,184)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          204..406
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(234..235,237..238,241,275,279,282,292..293,308,398,
                     401..402)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(243,246,250,255,260..261,263..264,268)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,339,345,359..360,363..364,367,381..382,
                     385..386,388..390,392..393,396,400)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(314,318,322,345,347,356,363,367,379,382..383,
                     386..389,392..393,396,400)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..427
                     /gene="usp"
                     /coded_by="XM_034324726.2:522..1805"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mmkkerpmts vtaiiqgtqa qhwsrgntwl sldnsnmsms svgpqspldm kpdtaslina
       61 gnfspsgpns pgsfnagchs nllstspsgq nkavapyppn hplsgskhlc sicgdrasgk
      121 hygvyscegc kgffkrtvrk dlsyacreek sciidkrqrn rcqycryqkc lamgmkreav
      181 qeerqrtker dqsevestss lhsdmpieri leaekrveck meqqgnyena vsqicnatnk
      241 qlfqlvawak hiphftslpl edqvlllrag wnelliasfs hrsidvkdgi vlatgitvhr
      301 nsaqqagvgt ifdrvlselv skmremkmdr telgclrsii lfnpevrglk siqevtllre
      361 kiyaalegyc rvawpddpgr faklllrlpa irsiglkcle hlfffkmigd vpiddflvem
      421 lesrsdp
//
LOCUS       XP_034180625             419 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180625
VERSION     XP_034180625.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324734.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..419
                     /product="retinoid X receptor ultraspiracle isoform X2"
                     /calculated_mol_wt=47016
     Region          100..176
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(102,105,119,122,138,144,154,157)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(107,148..149,151,153)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(111..114,120..121,123,125,127..128,131,139,151..152,
                     155,158,171..173,175..176)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(133,156,159..160,163..164,168..170,174..176)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(149..150,153)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            170..175
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(174,176)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          196..398
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(226..227,229..230,233,267,271,274,284..285,300,390,
                     393..394)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(235,238,242,247,252..253,255..256,260)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(306,310,314,331,337,351..352,355..356,359,373..374,
                     377..378,380..382,384..385,388,392)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(306,310,314,337,339,348,355,359,371,374..375,
                     378..381,384..385,388,392)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..419
                     /gene="usp"
                     /coded_by="XM_034324734.2:396..1655"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mmkkerpmts vtaiiqgtqa qhwsrgntwl sldnsnmsms svgpqspldm kpdtaslina
       61 gnfspsgpns pgnllstsps gqnkavapyp pnhplsgskh lcsicgdras gkhygvysce
      121 gckgffkrtv rkdlsyacre eksciidkrq rnrcqycryq kclamgmkre avqeerqrtk
      181 erdqsevest sslhsdmpie rileaekrve ckmeqqgnye navsqicnat nkqlfqlvaw
      241 akhiphftsl pledqvlllr agwnellias fshrsidvkd givlatgitv hrnsaqqagv
      301 gtifdrvlse lvskmremkm drtelgclrs iilfnpevrg lksiqevtll rekiyaaleg
      361 ycrvawpddp grfaklllrl pairsiglkc lehlfffkmi gdvpiddflv emlesrsdp
//
LOCUS       XP_034180633             414 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180633
VERSION     XP_034180633.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324742.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="retinoid X receptor ultraspiracle isoform X3"
                     /calculated_mol_wt=46448
     Region          108..184
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(110,113,127,130,146,152,162,165)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(115,156..157,159,161)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(119..122,128..129,131,133,135..136,139,147,159..160,
                     163,166,179..181,183..184)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(141,164,167..168,171..172,176..178,182..184)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(157..158,161)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            178..183
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(182,184)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          204..393
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(224..225,228,262,266,269,279..280,295,385,388..389)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(230,233,237,242,247..248,250..251,255)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(301,305,309,326,332,346..347,350..351,354,368..369,
                     372..373,375..377,379..380,383,387)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(301,305,309,332,334,343,350,354,366,369..370,
                     373..376,379..380,383,387)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..414
                     /gene="usp"
                     /coded_by="XM_034324742.2:396..1640"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mmkkerpmts vtaiiqgtqa qhwsrgntwl sldnsnmsms svgpqspldm kpdtaslina
       61 gnfspsgpns pgsfnagchs nllstspsgq nkavapyppn hplsgskhlc sicgdrasgk
      121 hygvyscegc kgffkrtvrk dlsyacreek sciidkrqrn rcqycryqkc lamgmkreav
      181 qeerqrtker dqsevestss lhsdmpieri leaekrveck meqqgnyelf qlvawakhip
      241 hftslpledq vlllragwne lliasfshrs idvkdgivla tgitvhrnsa qqagvgtifd
      301 rvlselvskm remkmdrtel gclrsiilfn pevrglksiq evtllrekiy aalegycrva
      361 wpddpgrfak lllrlpairs iglkclehlf ffkmigdvpi ddflvemles rsdp
//
LOCUS       XP_034180642             406 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180642
VERSION     XP_034180642.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324751.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="retinoid X receptor ultraspiracle isoform X4"
                     /calculated_mol_wt=45290
     Region          87..163
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(89,92,106,109,125,131,141,144)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(94,135..136,138,140)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(98..101,107..108,110,112,114..115,118,126,138..139,
                     142,145,158..160,162..163)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(120,143,146..147,150..151,155..157,161..163)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(136..137,140)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            157..162
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(161,163)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          183..385
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(213..214,216..217,220,254,258,261,271..272,287,377,
                     380..381)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(222,225,229,234,239..240,242..243,247)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(293,297,301,318,324,338..339,342..343,346,360..361,
                     364..365,367..369,371..372,375,379)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(293,297,301,324,326,335,342,346,358,361..362,
                     365..368,371..372,375,379)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..406
                     /gene="usp"
                     /coded_by="XM_034324751.2:16..1236"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mngiilkgls ldnsnmsmss vgpqspldmk pdtaslinag nfspsgpnsp gsfnagchsn
       61 llstspsgqn kavapyppnh plsgskhlcs icgdrasgkh ygvyscegck gffkrtvrkd
      121 lsyacreeks ciidkrqrnr cqycryqkcl amgmkreavq eerqrtkerd qsevestssl
      181 hsdmpieril eaekrveckm eqqgnyenav sqicnatnkq lfqlvawakh iphftslple
      241 dqvlllragw nelliasfsh rsidvkdgiv latgitvhrn saqqagvgti fdrvlselvs
      301 kmremkmdrt elgclrsiil fnpevrglks iqevtllrek iyaalegycr vawpddpgrf
      361 aklllrlpai rsiglkcleh lfffkmigdv piddflveml esrsdp
//
LOCUS       XP_034180649             405 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180649
VERSION     XP_034180649.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324758.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="retinoid X receptor ultraspiracle isoform X5"
                     /calculated_mol_wt=45240
     Region          86..162
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(88,91,105,108,124,130,140,143)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(93,134..135,137,139)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(97..100,106..107,109,111,113..114,117,125,137..138,
                     141,144,157..159,161..162)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(119,142,145..146,149..150,154..156,160..162)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(135..136,139)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            156..161
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(160,162)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          182..384
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(212..213,215..216,219,253,257,260,270..271,286,376,
                     379..380)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(221,224,228,233,238..239,241..242,246)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(292,296,300,317,323,337..338,341..342,345,359..360,
                     363..364,366..368,370..371,374,378)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(292,296,300,323,325,334,341,345,357,360..361,
                     364..367,370..371,374,378)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..405
                     /gene="usp"
                     /coded_by="XM_034324758.2:285..1502"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 messerglsl dnsnmsmssv gpqspldmkp dtaslinagn fspsgpnspg sfnagchsnl
       61 lstspsgqnk avapyppnhp lsgskhlcsi cgdrasgkhy gvyscegckg ffkrtvrkdl
      121 syacreeksc iidkrqrnrc qycryqkcla mgmkreavqe erqrtkerdq sevestsslh
      181 sdmpierile aekrveckme qqgnyenavs qicnatnkql fqlvawakhi phftslpled
      241 qvlllragwn elliasfshr sidvkdgivl atgitvhrns aqqagvgtif drvlselvsk
      301 mremkmdrte lgclrsiilf npevrglksi qevtllreki yaalegycrv awpddpgrfa
      361 klllrlpair siglkclehl fffkmigdvp iddflvemle srsdp
//
LOCUS       XP_076546045             397 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546045
VERSION     XP_076546045.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689930.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="retinoid X receptor ultraspiracle isoform X6"
                     /calculated_mol_wt=44436
     Region          78..154
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(80,83,97,100,116,122,132,135)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143514"
     Site            order(85,126..127,129,131)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(89..92,98..99,101,103,105..106,109,117,129..130,133,
                     136,149..151,153..154)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(111,134,137..138,141..142,146..148,152..154)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(127..128,131)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            148..153
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(152,154)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          174..376
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(204..205,207..208,211,245,249,252,262..263,278,368,
                     371..372)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(213,216,220,225,230..231,233..234,238)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(284,288,292,309,315,329..330,333..334,337,351..352,
                     355..356,358..360,362..363,366,370)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(284,288,292,315,317,326,333,337,349,352..353,
                     356..359,362..363,366,370)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..397
                     /gene="usp"
                     /coded_by="XM_076689930.1:288..1481"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 messerglsl dnsnmsmssv gpqspldmkp dtaslinagn fspsgpnspg nllstspsgq
       61 nkavapyppn hplsgskhlc sicgdrasgk hygvyscegc kgffkrtvrk dlsyacreek
      121 sciidkrqrn rcqycryqkc lamgmkreav qeerqrtker dqsevestss lhsdmpieri
      181 leaekrveck meqqgnyena vsqicnatnk qlfqlvawak hiphftslpl edqvlllrag
      241 wnelliasfs hrsidvkdgi vlatgitvhr nsaqqagvgt ifdrvlselv skmremkmdr
      301 telgclrsii lfnpevrglk siqevtllre kiyaalegyc rvawpddpgr faklllrlpa
      361 irsiglkcle hlfffkmigd vpiddflvem lesrsdp
//
LOCUS       XP_034180659             373 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid X receptor ultraspiracle isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180659
VERSION     XP_034180659.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324768.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="retinoid X receptor ultraspiracle isoform X7"
                     /calculated_mol_wt=42237
     Region          <73..130
                     /region_name="NR_DBD_RXR"
                     /note="DNA-binding domain of retinoid X receptor (RXR) is
                     composed of two C4-type zinc fingers; cd06956"
                     /db_xref="CDD:143514"
     Site            order(74..75,77,79,81..82,85,93,105..106,109,112,125..127,
                     129..130)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143514"
     Site            order(87,110,113..114,117..118,122..124,128..130)
                     /site_type="other"
                     /note="RXR/PPAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(102..103,105,107)
                     /site_type="other"
                     /note="RXR/ECR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(103..104,107)
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR1 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            124..129
                     /site_type="other"
                     /note="RXR/RXR homodimer interface DR2 [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Site            order(128,130)
                     /site_type="other"
                     /note="RXR/RAR heterodimer interface [polypeptide
                     binding]"
                     /db_xref="CDD:143514"
     Region          150..352
                     /region_name="NR_LBD_RXR_like"
                     /note="The ligand binding domain of the retinoid X
                     receptor and Ultraspiracle, members of nuclear receptor
                     superfamily; cd06943"
                     /db_xref="CDD:132741"
     Site            order(180..181,183..184,187,221,225,228,238..239,254,344,
                     347..348)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132741"
     Site            order(189,192,196,201,206..207,209..210,214)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(260,264,268,285,291,305..306,309..310,313,327..328,
                     331..332,334..336,338..339,342,346)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     Site            order(260,264,268,291,293,302,309,313,325,328..329,
                     332..335,338..339,342,346)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:132741"
     CDS             1..373
                     /gene="usp"
                     /coded_by="XM_034324768.2:396..1517"
                     /db_xref="GeneID:117604528"
ORIGIN      
        1 mmkkerpmts vtaiiqgtqa qhwsrgntwl sldnsnmsms svgpqspldm kpdtaslina
       61 gnfspsgpns pgcegckgff krtvrkdlsy acreekscii dkrqrnrcqy cryqkclamg
      121 mkreavqeer qrtkerdqse vestsslhsd mpierileae krveckmeqq gnyenavsqi
      181 cnatnkqlfq lvawakhiph ftslpledqv lllragwnel liasfshrsi dvkdgivlat
      241 gitvhrnsaq qagvgtifdr vlselvskmr emkmdrtelg clrsiilfnp evrglksiqe
      301 vtllrekiya alegycrvaw pddpgrfakl llrlpairsi glkclehlff fkmigdvpid
      361 dflvemlesr sdp
//
LOCUS       XP_034174032             552 aa            linear   INV 03-SEP-2025
DEFINITION  serine palmitoyltransferase long chain base subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034174032
VERSION     XP_034174032.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318141.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..552
                     /product="serine palmitoyltransferase long chain base
                     subunit"
                     /calculated_mol_wt=61858
     Region          66..535
                     /region_name="AAT_I"
                     /note="Aspartate aminotransferase (AAT) superfamily (fold
                     type I) of pyridoxal phosphate (PLP)-dependent enzymes.
                     PLP combines with an alpha-amino acid to form a compound
                     called a Schiff base or aldimine intermediate, which
                     depending on the reaction, is the...; cl18945"
                     /db_xref="CDD:450240"
     Site            order(170,231..233,257,309,313..314,338,340..341,370,373,
                     503)
                     /site_type="active"
                     /note="substrate-cofactor binding pocket [active]"
                     /db_xref="CDD:99747"
     Site            order(231..233,257,309,313,338,340..341,370,373)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99747"
     Site            373
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99747"
     CDS             1..552
                     /gene="lace"
                     /coded_by="XM_034318141.2:87..1745"
                     /db_xref="GeneID:117601430"
ORIGIN      
        1 matqtttrrr lneheekirs rngltfhegv langhagsqs ngrvqfpdke tksepvtsrs
       61 kesfervpfi taalthlgfy ilmflgfinq lfftpkvake ynregyaply ehfeefylry
      121 vyrrvrdcwn rpicsvpgat vtlkdritrd ygwtfqftgt ttecinlgsy nylgfaesdg
      181 kcadhsietl kkfgcttcss rlelgnmpih eeleqltarf lgvedaivfg mgfatnalnl
      241 psfltkgclv lsdeknhasl ilglrlscst vrvfkhndvk hletclqnai vygqpgsgep
      301 wkkilivveg vysmegsivh lpeiielkkk ykaylyldea hstgalgkhg rgvcdyygid
      361 prevdilmgt ftksfgsagg yiagsktlin hlriyshaht yasamsppva qqiitsmkii
      421 agedgtdege krtkqlarnt kyfrrrlnqi gviiygneds pvvpmlvylf skigavvrtl
      481 ttyniaavgv gfpatplmeg rirfclsaah tkqqldyvls riesladslg lrysrkfrdp
      541 apieyysdge te
//
LOCUS       XP_034180671             363 aa            linear   INV 03-SEP-2025
DEFINITION  integrator complex subunit 15 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034180671
VERSION     XP_034180671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..363
                     /product="integrator complex subunit 15 isoform X1"
                     /calculated_mol_wt=40164
     Region          5..346
                     /region_name="DUF4507"
                     /note="Domain of unknown function (DUF4507); pfam14964"
                     /db_xref="CDD:464409"
     CDS             1..363
                     /gene="LOC117604562"
                     /coded_by="XM_034324780.2:199..1290"
                     /db_xref="GeneID:117604562"
ORIGIN      
        1 maggdikfnl rnldfpycat ealcrieilc gksgkqmdlq ldlisefvfg eierrkkrnm
       61 tlaiqelqli evlsdffqnp ggmpavrnai flslfpadsp rykilgnlvs laiatqnkai
      121 lnatgiwmqq lgstspqsvg larhvlndyf vltpksidkl kqlpvlashf tanlltaige
      181 vyedkdppte llrligewid enpgllltpl mdnpalptgg ipmtpitpia glfrwcilsp
      241 lrvntvenae nseeqrklys kiqqllmdsv lrlknsrnnk haisaqhfaa ttrtlttslq
      301 sqvnvsstlh dlamarlaqa vsaamsanci ygnkqellal lqplsyqhfl iewtlqtyas
      361 kat
//
LOCUS       XP_034180681             362 aa            linear   INV 03-SEP-2025
DEFINITION  integrator complex subunit 15 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034180681
VERSION     XP_034180681.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324790.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..362
                     /product="integrator complex subunit 15 isoform X2"
                     /calculated_mol_wt=40035
     Region          5..345
                     /region_name="DUF4507"
                     /note="Domain of unknown function (DUF4507); pfam14964"
                     /db_xref="CDD:464409"
     CDS             1..362
                     /gene="LOC117604562"
                     /coded_by="XM_034324790.2:206..1294"
                     /db_xref="GeneID:117604562"
ORIGIN      
        1 maggdikfnl rnldfpycat ealcrieilc gksgkqmdlq ldlisefvfg eierrkkrnm
       61 tlaiqelqli evlsdffqnp ggmpavrnai flslfpadsp rykilgnlvs laiatqnkai
      121 lnatgiwmqq lgstspqsvg larhvlndyf vltpksidkl kqlpvlashf tanlltaige
      181 vyedkdppte llrligewid enpgllltpl mdnpalptgg ipmtpitpia glfrwcilsp
      241 lrvntvenae nseeqrklys kiqqllmdsv lrlknsrnnk haisaqhfaa ttrtlttslq
      301 sqvnvsstlh dlamarlaqa vsaamsanci ygnkqllall qplsyqhfli ewtlqtyask
      361 at
//
LOCUS       XP_034180691             271 aa            linear   INV 03-SEP-2025
DEFINITION  jun-related antigen [Osmia lignaria lignaria].
ACCESSION   XP_034180691
VERSION     XP_034180691.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324800.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..271
                     /product="jun-related antigen"
                     /calculated_mol_wt=30128
     Region          9..>138
                     /region_name="Jun"
                     /note="Jun-like transcription factor; pfam03957"
                     /db_xref="CDD:461108"
     Region          196..256
                     /region_name="bZIP_Jun"
                     /note="Basic leucine zipper (bZIP) domain of Jun proteins
                     and similar proteins: a DNA-binding and dimerization
                     domain; cd14696"
                     /db_xref="CDD:269844"
     Region          197..248
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269844"
     Site            order(201,203..205,207..212,214..216)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269844"
     Site            order(215..216,218..219,221..223,225..226,229..230,
                     232..233,235..237,239..240,243..244,247,250,253..256)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269844"
     CDS             1..271
                     /gene="Jra"
                     /coded_by="XM_034324800.2:646..1461"
                     /db_xref="GeneID:117604570"
ORIGIN      
        1 mvrssttmeq tfyeetsvyg vvnrennvgq lkrnltldln gcqrqgpqak rprlgplppa
       61 lnnvapilss pdlnmlklgs pelekfiiaq qdslvtnlpt ptqilfpkav teaqelyarg
      121 fvealnelhh sdssqepgsi hgatyttlep pssvqstess vsqglmqikd epqtvpsvss
      181 sppmspidme nqeriklerk rqrnrvaask crrrkleris rledkvkllk gentelsavv
      241 hklkehvcrl keqvmdhvhs gcqimavsgq f
//
LOCUS       XP_034180706             255 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal RNA-processing protein 7 homolog A [Osmia lignaria
            lignaria].
ACCESSION   XP_034180706
VERSION     XP_034180706.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324815.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180706.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="ribosomal RNA-processing protein 7 homolog A"
                     /calculated_mol_wt=30056
     Region          48..131
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          123..255
                     /region_name="RRP7_Rrp7A"
                     /note="RRP7 domain ribosomal RNA-processing protein 7
                     homolog A (Rrp7A) and similar proteins; cd12951"
                     /db_xref="CDD:240578"
     CDS             1..255
                     /gene="LOC117604573"
                     /coded_by="XM_034324815.2:227..994"
                     /db_xref="GeneID:117604573"
ORIGIN      
        1 mimksekqnl kgfktlwiqf nrkntdkhql flkehsirnq lpehpkgrtl fvlnvppyvt
       61 pealqkafsq icgkvtnvff vtskgfktay vvfekettle kalelpanhm islnrkdnvc
      121 ltglakwcke ynesicdeek lkkeiedyml dydkkiaekk anekaieeea edndgwvtvs
      181 arkkrgeyal srkestinkv qhkeeqrnkk kqllnfytfq ireskkqnla elrkkfeldk
      241 qrlqslkskr tfrpf
//
LOCUS       XP_034180721             239 aa            linear   INV 03-SEP-2025
DEFINITION  spermiogenesis-related protein stanley-cup isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180721
VERSION     XP_034180721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324830.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="spermiogenesis-related protein stanley-cup
                     isoform X1"
                     /calculated_mol_wt=25644
     CDS             1..239
                     /gene="s-cup"
                     /coded_by="XM_034324830.2:599..1318"
                     /db_xref="GeneID:117604578"
ORIGIN      
        1 mshessprtk rttfrrtmti iaaaeatsnk rnatnvhedv ailpgynvtt tasgasskts
       61 hfetdlhgnv nillggamls gvimvvflmc ycchksirks rpqeypqywr tepdvhslev
      121 ftteahtacc erqstaegnh destygalsc pvaprsgpgp pptydslifd pkslpvsiek
      181 kmdgpetpgt iipsmvssll glasspvecn esseqetaat deglpsyeaa lkldsngyv
//
LOCUS       XP_076546046             239 aa            linear   INV 03-SEP-2025
DEFINITION  spermiogenesis-related protein stanley-cup isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546046
VERSION     XP_076546046.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689931.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="spermiogenesis-related protein stanley-cup
                     isoform X1"
                     /calculated_mol_wt=25644
     CDS             1..239
                     /gene="s-cup"
                     /coded_by="XM_076689931.1:1614..2333"
                     /db_xref="GeneID:117604578"
ORIGIN      
        1 mshessprtk rttfrrtmti iaaaeatsnk rnatnvhedv ailpgynvtt tasgasskts
       61 hfetdlhgnv nillggamls gvimvvflmc ycchksirks rpqeypqywr tepdvhslev
      121 ftteahtacc erqstaegnh destygalsc pvaprsgpgp pptydslifd pkslpvsiek
      181 kmdgpetpgt iipsmvssll glasspvecn esseqetaat deglpsyeaa lkldsngyv
//
LOCUS       XP_034180738             235 aa            linear   INV 03-SEP-2025
DEFINITION  spermiogenesis-related protein stanley-cup isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180738
VERSION     XP_034180738.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324847.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="spermiogenesis-related protein stanley-cup
                     isoform X2"
                     /calculated_mol_wt=25241
     CDS             1..235
                     /gene="s-cup"
                     /coded_by="XM_034324847.2:606..1313"
                     /db_xref="GeneID:117604578"
ORIGIN      
        1 mshessprtk rttfrrtmti iaaaeatsnk rnatnvhedv ailpgynvtt tasgasskts
       61 hfetdlhgnv nillgggvim vvflmcycch ksirksrpqe ypqywrtepd vhslevftte
      121 ahtaccerqs taegnhdest ygalscpvap rsgpgpppty dslifdpksl pvsiekkmdg
      181 petpgtiips mvssllglas spvecnesse qetaatdegl psyeaalkld sngyv
//
LOCUS       XP_034180747             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604593 [Osmia lignaria lignaria].
ACCESSION   XP_034180747
VERSION     XP_034180747.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324856.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180747.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117604593"
                     /calculated_mol_wt=26504
     Region          150..218
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     CDS             1..228
                     /gene="LOC117604593"
                     /coded_by="XM_034324856.2:287..973"
                     /db_xref="GeneID:117604593"
ORIGIN      
        1 mnwtfwvfvi gsfsckyvna qvqrvppgvs pqhyqqpvqq vhqqipqqvp qqvpqqqyqq
       61 vpgqvpmhqv pvqqqqhhhq qqqqqqqqqq qqqhmqppqv svqsghhhdh sqpilnaani
      121 vnekahiaeh mevpmdtskm teqelqfhyf kmhdadnnnk ldgceliksl ihwheqgnke
      181 vreklfkdde lvtlidpils mddtnndgyi dypefiqaqq faaatgrq
//
LOCUS       XP_034180759             223 aa            linear   INV 03-SEP-2025
DEFINITION  UFM1 specific peptidase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034180759
VERSION     XP_034180759.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324868.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180759.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="UFM1 specific peptidase 1"
                     /calculated_mol_wt=25650
     Region          23..209
                     /region_name="Peptidase_C78"
                     /note="Peptidase family C78; pfam07910"
                     /db_xref="CDD:400318"
     CDS             1..223
                     /gene="Ufsp1"
                     /coded_by="XM_034324868.2:164..835"
                     /db_xref="GeneID:117604601"
ORIGIN      
        1 mihdyssell knihwdvspp evgtsflvrg dyeywhygcd gfndkgwgcg yrtlqticsw
       61 iimnrkldqs vpnirriqei lvtledkees figsrewigs fevclvldhl yevlskiihv
      121 psgralseqi pvirehlekf gspimmggdr dcsskgilgi hqgvkntyml ivdphfigrt
      181 knaeqlevnh wvkwqdtknf vdssfynlcl pqikcitsnk qed
//
LOCUS       XP_034180770             223 aa            linear   INV 03-SEP-2025
DEFINITION  UFM1 specific peptidase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034180770
VERSION     XP_034180770.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324879.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180770.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="UFM1 specific peptidase 1"
                     /calculated_mol_wt=25650
     Region          23..209
                     /region_name="Peptidase_C78"
                     /note="Peptidase family C78; pfam07910"
                     /db_xref="CDD:400318"
     CDS             1..223
                     /gene="Ufsp1"
                     /coded_by="XM_034324879.2:160..831"
                     /db_xref="GeneID:117604601"
ORIGIN      
        1 mihdyssell knihwdvspp evgtsflvrg dyeywhygcd gfndkgwgcg yrtlqticsw
       61 iimnrkldqs vpnirriqei lvtledkees figsrewigs fevclvldhl yevlskiihv
      121 psgralseqi pvirehlekf gspimmggdr dcsskgilgi hqgvkntyml ivdphfigrt
      181 knaeqlevnh wvkwqdtknf vdssfynlcl pqikcitsnk qed
//
LOCUS       XP_034180779             223 aa            linear   INV 03-SEP-2025
DEFINITION  UFM1 specific peptidase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034180779
VERSION     XP_034180779.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324888.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180779.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="UFM1 specific peptidase 1"
                     /calculated_mol_wt=25650
     Region          23..209
                     /region_name="Peptidase_C78"
                     /note="Peptidase family C78; pfam07910"
                     /db_xref="CDD:400318"
     CDS             1..223
                     /gene="Ufsp1"
                     /coded_by="XM_034324888.2:170..841"
                     /db_xref="GeneID:117604601"
ORIGIN      
        1 mihdyssell knihwdvspp evgtsflvrg dyeywhygcd gfndkgwgcg yrtlqticsw
       61 iimnrkldqs vpnirriqei lvtledkees figsrewigs fevclvldhl yevlskiihv
      121 psgralseqi pvirehlekf gspimmggdr dcsskgilgi hqgvkntyml ivdphfigrt
      181 knaeqlevnh wvkwqdtknf vdssfynlcl pqikcitsnk qed
//
LOCUS       XP_034180788             223 aa            linear   INV 03-SEP-2025
DEFINITION  UFM1 specific peptidase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034180788
VERSION     XP_034180788.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324897.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180788.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="UFM1 specific peptidase 1"
                     /calculated_mol_wt=25650
     Region          23..209
                     /region_name="Peptidase_C78"
                     /note="Peptidase family C78; pfam07910"
                     /db_xref="CDD:400318"
     CDS             1..223
                     /gene="Ufsp1"
                     /coded_by="XM_034324897.2:169..840"
                     /db_xref="GeneID:117604601"
ORIGIN      
        1 mihdyssell knihwdvspp evgtsflvrg dyeywhygcd gfndkgwgcg yrtlqticsw
       61 iimnrkldqs vpnirriqei lvtledkees figsrewigs fevclvldhl yevlskiihv
      121 psgralseqi pvirehlekf gspimmggdr dcsskgilgi hqgvkntyml ivdphfigrt
      181 knaeqlevnh wvkwqdtknf vdssfynlcl pqikcitsnk qed
//
LOCUS       XP_034174243             217 aa            linear   INV 03-SEP-2025
DEFINITION  trafficking protein particle complex subunit 4-like protein Trs23
            [Osmia lignaria lignaria].
ACCESSION   XP_034174243
VERSION     XP_034174243.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318352.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="trafficking protein particle complex subunit
                     4-like protein Trs23"
                     /calculated_mol_wt=24288
     Region          99..205
                     /region_name="TRAPPC4_synbindin"
                     /note="Trafficking protein particle complex subunit 4;
                     cd14856"
                     /db_xref="CDD:341446"
     Site            order(110..111,114..115,118,121..128,132..139,142,144,158)
                     /site_type="other"
                     /note="TRAPPC1 interface [polypeptide binding]"
                     /db_xref="CDD:341446"
     Site            order(132,148..153,171,174..175,177..179,182..184,187)
                     /site_type="other"
                     /note="TRAPPC3 interface [polypeptide binding]"
                     /db_xref="CDD:341446"
     Site            order(179,182..185,197)
                     /site_type="other"
                     /note="TRAPPC5 interface [polypeptide binding]"
                     /db_xref="CDD:341446"
     CDS             1..217
                     /gene="Trs23"
                     /coded_by="XM_034318352.2:1..654"
                     /db_xref="GeneID:117601515"
ORIGIN      
        1 mviygvyivs ksgglifnhd hnvprienek tfnypldikl gyenkkvvvs fgqkdginvg
       61 haltavngsp vvgrelengk dvfelleqpe nfpvslrfsr armttnekif lasmfyplfa
      121 iasqlspepr csgieilead tfrlhcyqtl tgikfivvae ptqsgieill krvfelyady
      181 alknpfysle mpircelfes nlqtllenve ksgagtv
//
LOCUS       XP_034180803             172 aa            linear   INV 03-SEP-2025
DEFINITION  translationally controlled tumor protein [Osmia lignaria lignaria].
ACCESSION   XP_034180803
VERSION     XP_034180803.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324912.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..172
                     /product="translationally controlled tumor protein"
                     /calculated_mol_wt=19728
     Region          1..167
                     /region_name="TCTP"
                     /note="Translationally controlled tumour protein;
                     pfam00838"
                     /db_xref="CDD:459957"
     CDS             1..172
                     /gene="Tctp"
                     /coded_by="XM_034324912.2:129..647"
                     /db_xref="GeneID:117604615"
ORIGIN      
        1 mriykdiftg demfsdtykm kliddvlyev ygrlvtrkag dieiagfnps seeadegtde
       61 avesgvdvvl nhrlqetfaf adkksytlyl kdymkklvar leekapdqve vfktntnkvm
      121 kdilsrfkdl qfftgesmdi dgivalmeyr didgesvpvl mlfkhgleee kf
//
LOCUS       XP_034174257             161 aa            linear   INV 03-SEP-2025
DEFINITION  gem-associated protein 6 [Osmia lignaria lignaria].
ACCESSION   XP_034174257
VERSION     XP_034174257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="gem-associated protein 6"
                     /calculated_mol_wt=18095
     Region          16..75
                     /region_name="Gemin6"
                     /note="Gemin 6; cd11676"
                     /db_xref="CDD:212487"
     Site            order(16..17,19..20,23..24,32..33,35,37,42..47,50,52,
                     60..66,68)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:212487"
     Region          99..156
                     /region_name="Gemin6_C"
                     /note="Gemin6 C-terminal domain; pfam20417"
                     /db_xref="CDD:466566"
     CDS             1..161
                     /gene="LOC117601519"
                     /coded_by="XM_034318366.2:1..486"
                     /db_xref="GeneID:117601519"
ORIGIN      
        1 meesshfshn iykndpvlfk nyvgfkvnil tedgttssgv vytvdpvses vvllqdcqqd
       61 hlkivfghai knieicmdkp cdlpelfmna ptnilrstle erknavikil renrfdvkee
      121 sdiltvenvl sikppyeptd cicansiilg riqnllsris t
//
LOCUS       XP_034180809             157 aa            linear   INV 03-SEP-2025
DEFINITION  evr1_Alr domain-containing protein Alr [Osmia lignaria lignaria].
ACCESSION   XP_034180809
VERSION     XP_034180809.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324918.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180809.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..157
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..157
                     /product="evr1_Alr domain-containing protein Alr"
                     /calculated_mol_wt=18323
     Region          58..148
                     /region_name="Evr1_Alr"
                     /note="Erv1 / Alr family; pfam04777"
                     /db_xref="CDD:461423"
     CDS             1..157
                     /gene="Alr"
                     /coded_by="XM_034324918.2:58..531"
                     /db_xref="GeneID:117604621"
ORIGIN      
        1 mshekpcrac tdfktwaksq kktfdstkes eeskkkdqav nntrrecpld kdelgsrtws
       61 flhtmaayyp dnpsdeqksd mktffkifsk fypcyvcaed lqeqikqypp qtdsqeklsq
      121 wlcnihngvn krlgkplfdc klvnqrwkdg wldgscd
//
LOCUS       XP_034173991            1182 aa            linear   INV 03-SEP-2025
DEFINITION  intraflagellar transport protein Oseg4 [Osmia lignaria lignaria].
ACCESSION   XP_034173991
VERSION     XP_034173991.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318100.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1182
                     /product="intraflagellar transport protein Oseg4"
                     /calculated_mol_wt=133744
     Region          <16..188
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          18..73
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          69..332
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(74,92,96,102..103,118..119,136,140,146..147,
                     156..157,176,181,187..188,205,230,235,241,243,255..256,
                     272,286,292..293,305..306,322,327)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          78..115
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          123..161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          163..201
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          223..254
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          311..338
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          380..417
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <728..936
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          765..791
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          798..824
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(800..801,804..805,807,830,833..834,867..868,
                     876..877,911,914..915,918..919,922)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          828..900
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          910..936
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..1182
                     /gene="Oseg4"
                     /coded_by="XM_034318100.2:108..3656"
                     /db_xref="GeneID:117601404"
ORIGIN      
        1 mfvylskkia ipnnfrlnci awnqregyia vggedgllkv lrvdssvsgs ssggksrnlt
       61 aasnlsmnqt leghnshvqv vtwneqhqkl tssdqngvii vwmlykgswy deminncnks
      121 ivkgmawssn glkicivyed gavivgsvdg nriwgkelkn islaavqwsp dgklllfglk
      181 ngevhlydnq gvfltklqmv slnsqiqtiv slqwydgrng yigldcptli icyrngkiql
      241 mrdtnddnpi vietgmtaaw ccwnscgsll avtgmmpmls ngetkdtnvi qfytpfgkhm
      301 ktlkipgrev tccaweggsl rvalsvdshi yfanirldyk wtyfsntvvy tnekiskdgi
      361 cimfwntvnn tcctkyvkal isvssygdhc vlavkndlvq gseqfallvc nsiatpidtk
      421 fidleplwvt mtnntiitas knnflvwnhr tprntmlhag rvkrdkiyhi detptgvtev
      481 iqdldkdrsf etpintkatm dpicclyate kvlligresg miqryslpqi tltnryntsc
      541 klykiaincd ssraslmdts gvltmldldi dlkknfkngd teddinkfer kdvwamcwaq
      601 dnpallaime ktrmyvlref dpeepiscsg yicsfqdlei rcvllddlmq kpddtrneli
      661 vdlevkslrd trelltkvgl keannfiqdn phprlwrlla esalqkldle taenamvrct
      721 dylgiqfikr lqnvhndqlk raevagflgn ydeaeklyld mdrrdlaisl rqklgdyfrv
      781 iqlmkmgigg sdkqtehayn kigeyyaerq nwegareyye ksrnleklid cyykledfvq
      841 lagtvqqlsd kspllktvar mlasvgmcsq avaayikygd vklavdtcvr lnhwdqavel
      901 artykmaqig ellnkyanhl lsngkmlqai elykkanynl eaakllfqla eeqamsrtnp
      961 lrvkkiyvla alliedhini tpatkggrsn ivmgltenne dsrvienawr gaeayhflll
     1021 ahrqvysghy daamktalrl reyedilqpe diycllalss avnhafavcs kafiklesle
     1081 iisesareey edlavdiftk hspkdvrnsk aecancetmv pdwcvacpnc mtrfppciis
     1141 gkplmdlsna wictvcrhhv aterdvvnin acplchstvt ym
//
LOCUS       XP_034174004             911 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear polyadenosine RNA-binding 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174004
VERSION     XP_034174004.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318113.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174004.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..911
                     /product="nuclear polyadenosine RNA-binding 2 isoform X1"
                     /calculated_mol_wt=103782
     Region          815..832
                     /region_name="zf-CCCH_2"
                     /note="RNA-binding, Nab2-type zinc finger; pfam14608"
                     /db_xref="CDD:464217"
     Region          836..853
                     /region_name="zf-CCCH_2"
                     /note="RNA-binding, Nab2-type zinc finger; pfam14608"
                     /db_xref="CDD:464217"
     Region          874..891
                     /region_name="zf-CCCH_2"
                     /note="RNA-binding, Nab2-type zinc finger; pfam14608"
                     /db_xref="CDD:464217"
     CDS             1..911
                     /gene="Nab2"
                     /coded_by="XM_034318113.2:236..2971"
                     /db_xref="GeneID:117601407"
ORIGIN      
        1 mdirgievtn qlrsairakl lelgvrydee lpdyilvmvv nkksrqqmhe dlylflesct
       61 tpfvdwlhdq vlkklqkvtv akkkslrefv ptvvvkqeee rkkkkactts flddqtvnqt
      121 tdkssnknlk ddksfhkqnt kasissqkld ttqnknidks vknisheskl dgtpnhvvsq
      181 qpvikrtqvt tdnqsvgvnk evftietlnn ksgdppkvdd kssrnlkrpl emtnnykdnl
      241 ekkqteakrs kieengedtt ednakklksc vnkpkvtsvv svknrlgvvs prkkfevhre
      301 rmdnesryrq nekhlekhrl dsnrssnfqr grnfdaders krnretdlkh nesdkvinik
      361 trlgntkidk tpksidlkek vnsivksrsv ektggtikdr lgianklpkq lgnkepidfk
      421 skpleqdrdp sainkniknr lgplknnfkp lniksafnss irksqssedd dylnlgaeee
      481 ldddaqsvgn sgpikshiia vnkpapikte rkrlkslerg nkqssdlded meetkipskv
      541 ivtprplkpl qpaqkratqs lllravaean qsvvtqknpe pslldkkqtl krlkpapvrd
      601 vgqnlsvhln snkrlvmeki qvelntdite mkakpyvpqp vteehmgvvm slfqrsddnq
      661 kflvtlngyn nnhvneknms ddeerlemeg splfstqvne ddelalstiq ndmyvqnese
      721 tsvfqiteae eegdlkertd ennencnten vekieepprr rrklspivyn rshspspvdl
      781 ksntlvtssv ltkrlekkas tensvtvidk skekcrywpn ctlgnkcsyl hpavmcsafp
      841 ackfgdkcay khpkckfgls ctklgcvfsh paqqckyhpf ctkpacpysh pmpatvepss
      901 qrakftwrrr d
//
LOCUS       XP_034174013             903 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear polyadenosine RNA-binding 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174013
VERSION     XP_034174013.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174013.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..903
                     /product="nuclear polyadenosine RNA-binding 2 isoform X2"
                     /calculated_mol_wt=102964
     Region          807..824
                     /region_name="zf-CCCH_2"
                     /note="RNA-binding, Nab2-type zinc finger; pfam14608"
                     /db_xref="CDD:464217"
     Region          828..845
                     /region_name="zf-CCCH_2"
                     /note="RNA-binding, Nab2-type zinc finger; pfam14608"
                     /db_xref="CDD:464217"
     Region          866..883
                     /region_name="zf-CCCH_2"
                     /note="RNA-binding, Nab2-type zinc finger; pfam14608"
                     /db_xref="CDD:464217"
     CDS             1..903
                     /gene="Nab2"
                     /coded_by="XM_034318122.2:235..2946"
                     /db_xref="GeneID:117601407"
ORIGIN      
        1 mdirgievtn qlrsairakl lelgvrydee lpdyilvmvv nkksrqqmhe dlylflesct
       61 tpfvdwlhdq vlkklqkvtv akkkslrefv ptvvvkqeee rkkkkactts flddqtvnqt
      121 tdkssnknlk ddksfhkqnt kasissqkld ttqnknidks vknisheskl dgtpnhvvsq
      181 qpvikrtqvt tdnqsvgvnk evftietlnn ksgdppkvdd kssrnlkrpl emtnnykdnl
      241 ekkqteakrs kieengedtt ednakklksc vnkpkvtsvv svknrlgvvs prkkfevhre
      301 rmdnesryrq nekhlekhrl dsnrssnfqr grnfdaders krnretdlkh nesdkvinik
      361 trlgntkidk tpksidlkek vnsivksrsv ektggtikdr lgianklpkq lgnkepidfk
      421 skpleqdrdp sainkniknr lgplknnfkp lniksafnss irksqssedd dylnlgaeee
      481 ldddaqsvgn sgpikshiia vnkpapikte rkrlkslerg nkqssdlded meetkipskv
      541 ivtprplkpl qpaqkratqs lllravaean qsvvtqknpe pslldkkqtl krlkpapvrd
      601 vgqnlsvhln snkrlvmeki qvelntdite mkakpyvpqp vteehmgvvm slfqrsddnq
      661 kflvtlngyn nnhvneknms ddeerlemev neddelalst iqndmyvqne setsvfqite
      721 aeeegdlker tdennencnt envekieepp rrrrklspiv ynrshspspv dlksntlvts
      781 svltkrlekk astensvtvi dkskekcryw pnctlgnkcs ylhpavmcsa fpackfgdkc
      841 aykhpkckfg lsctklgcvf shpaqqckyh pfctkpacpy shpmpatvep ssqrakftwr
      901 rrd
//
LOCUS       XP_034174021             598 aa            linear   INV 03-SEP-2025
DEFINITION  glypican dally-like [Osmia lignaria lignaria].
ACCESSION   XP_034174021
VERSION     XP_034174021.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318130.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174021.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..598
                     /product="glypican dally-like"
                     /calculated_mol_wt=66685
     Region          7..590
                     /region_name="Glypican"
                     /note="pfam01153"
                     /db_xref="CDD:460084"
     CDS             1..598
                     /gene="dlp"
                     /coded_by="XM_034318130.2:281..2077"
                     /db_xref="GeneID:117601423"
ORIGIN      
        1 msgmlsmlcv ililsvgttt vraglkcdav rpyfesqgfp vsdipkeais skelkicgsv
       61 rsggevccsa dmevrlqara rdkhekatke tlqrlhqvls trgtrfhnff kdllatskrg
      121 fhemfkktyg ilyeqnayvf tdlfkeleny yakgtvdldd lmdnffntly qkmftvlnsq
      181 ynfdnkylec lgdhmkemrp fgdvpqklgv qikrsfvatr afsqaltvaa dvlknmqslk
      241 psadcaaalt rmtvcpscsg itgnvlacgd mcanvmkgcl aqhaaldaew nhfveavdkv
      301 adrllgpfni emlvrpinlk iseaimnfqe nshdvsqrvf tgcgrpvlgr rrrrdnrele
      361 leslnfdqdt ltedrastaa ildklvketr qrvrdsrqfw vllpyklcnd glvvppsntk
      421 ecwngthvdk yiypvssnge tqilnpevrs sgprptivrd qiyalttitn rlksafngqd
      481 vdwidtedte wngsgsgsgd ttdqdpitdd edgfkegsgy epkpsppevp kqsppavnpd
      541 vvpprvdvep kmngnsnngt stvdngasrq kmslsraltt ylvpivvmwf ggcltewl
//
LOCUS       XP_034174092             442 aa            linear   INV 03-SEP-2025
DEFINITION  spermatogenesis-defective protein 39 homolog isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174092
VERSION     XP_034174092.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318201.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174092.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..442
                     /product="spermatogenesis-defective protein 39 homolog
                     isoform X1"
                     /calculated_mol_wt=50287
     CDS             1..442
                     /gene="LOC117601464"
                     /coded_by="XM_034318201.2:66..1394"
                     /db_xref="GeneID:117601464"
ORIGIN      
        1 mteihlgkdi inmssakdde dfwyssekrs fcfennevdq lfgvsktdtd klwagisnts
       61 tseaslktts dyqplkpmls visektlscs msvksstila ldklqnlqpe ptvvqpditl
      121 rkillgqsys lepyksiask talldaaiis gdgnailvii lfltntlkrs lvqrilaerp
      181 davnvyiryl strmqineit diltmlgqtm daamktlhii ikntrdpdrl lqklrnsykt
      241 qfsalndcke asfvqsyiql lewqtvvkkv dgneeielns svldclrhac kshwnvaegt
      301 lmsptrlsqq hdisprqyqk valevrtsag ewddidklll tkgwlnskkl qihlpiedil
      361 kilqkskaps svlekylkyv dnierrlela rnmhcfriai dilvqqadra tlmeyktklq
      421 pqseeyfyae salrlpsvkw kn
//
LOCUS       XP_034174101             434 aa            linear   INV 03-SEP-2025
DEFINITION  spermatogenesis-defective protein 39 homolog isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174101
VERSION     XP_034174101.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318210.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174101.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..434
                     /product="spermatogenesis-defective protein 39 homolog
                     isoform X2"
                     /calculated_mol_wt=49479
     CDS             1..434
                     /gene="LOC117601464"
                     /coded_by="XM_034318210.2:66..1370"
                     /db_xref="GeneID:117601464"
ORIGIN      
        1 mteihlgkdi inmssakdde dfwyssekrs fcfennevdq lfgvsktdtd klwagisnts
       61 tseaslktts dyqplkpmls visektlsci laldklqnlq peptvvqpdi tlrkillgqs
      121 yslepyksia sktalldaai isgdgnailv iilfltntlk rslvqrilae rpdavnvyir
      181 ylstrmqine itdiltmlgq tmdaamktlh iiikntrdpd rllqklrnsy ktqfsalndc
      241 keasfvqsyi qllewqtvvk kvdgneeiel nssvldclrh ackshwnvae gtlmsptrls
      301 qqhdisprqy qkvalevrts agewddidkl lltkgwlnsk klqihlpied ilkilqkska
      361 pssvlekylk yvdnierrle larnmhcfri aidilvqqad ratlmeyktk lqpqseeyfy
      421 aesalrlpsv kwkn
//
LOCUS       XP_034174108             430 aa            linear   INV 03-SEP-2025
DEFINITION  spermatogenesis-defective protein 39 homolog isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174108
VERSION     XP_034174108.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318217.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174108.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="spermatogenesis-defective protein 39 homolog
                     isoform X3"
                     /calculated_mol_wt=48921
     CDS             1..430
                     /gene="LOC117601464"
                     /coded_by="XM_034318217.2:116..1408"
                     /db_xref="GeneID:117601464"
ORIGIN      
        1 mssakddedf wyssekrsfc fennevdqlf gvsktdtdkl wagisntsts easlkttsdy
       61 qplkpmlsvi sektlscsms vksstilald klqnlqpept vvqpditlrk illgqsysle
      121 pyksiaskta lldaaiisgd gnailviilf ltntlkrslv qrilaerpda vnvyirylst
      181 rmqineitdi ltmlgqtmda amktlhiiik ntrdpdrllq klrnsyktqf salndckeas
      241 fvqsyiqlle wqtvvkkvdg neeielnssv ldclrhacks hwnvaegtlm sptrlsqqhd
      301 isprqyqkva levrtsagew ddidkllltk gwlnskklqi hlpiedilki lqkskapssv
      361 lekylkyvdn ierrlelarn mhcfriaidi lvqqadratl meyktklqpq seeyfyaesa
      421 lrlpsvkwkn
//
LOCUS       XP_034174125             430 aa            linear   INV 03-SEP-2025
DEFINITION  spermatogenesis-defective protein 39 homolog isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174125
VERSION     XP_034174125.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318234.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174125.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="spermatogenesis-defective protein 39 homolog
                     isoform X3"
                     /calculated_mol_wt=48921
     CDS             1..430
                     /gene="LOC117601464"
                     /coded_by="XM_034318234.2:21..1313"
                     /db_xref="GeneID:117601464"
ORIGIN      
        1 mssakddedf wyssekrsfc fennevdqlf gvsktdtdkl wagisntsts easlkttsdy
       61 qplkpmlsvi sektlscsms vksstilald klqnlqpept vvqpditlrk illgqsysle
      121 pyksiaskta lldaaiisgd gnailviilf ltntlkrslv qrilaerpda vnvyirylst
      181 rmqineitdi ltmlgqtmda amktlhiiik ntrdpdrllq klrnsyktqf salndckeas
      241 fvqsyiqlle wqtvvkkvdg neeielnssv ldclrhacks hwnvaegtlm sptrlsqqhd
      301 isprqyqkva levrtsagew ddidkllltk gwlnskklqi hlpiedilki lqkskapssv
      361 lekylkyvdn ierrlelarn mhcfriaidi lvqqadratl meyktklqpq seeyfyaesa
      421 lrlpsvkwkn
//
LOCUS       XP_076546047             310 aa            linear   INV 03-SEP-2025
DEFINITION  short-chain dehydrogenase/reductase family 16C member 6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546047
VERSION     XP_076546047.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689932.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="short-chain dehydrogenase/reductase family 16C
                     member 6"
                     /calculated_mol_wt=34448
     Region          61..283
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(66,68..69,71,90..92,162..164,203..205,213,217,
                     239..242)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187598"
     CDS             1..310
                     /gene="LOC117601492"
                     /coded_by="XM_076689932.1:746..1678"
                     /db_xref="GeneID:117601492"
ORIGIN      
        1 miysrdeali vnprmiypss tfwlfftvef ligallsafl aalallksll pkpprdltgd
       61 vvvvagassp lgkslaeefa kkgctvvcid sdsrsiqeta crlkatrpsv eeitprhrkd
      121 dssrhesmiy ayqcdlidrn dirrtaerik edigrvdilv tcvgnpnqdi fdatsrtlms
      181 hfwtamafvp lmlyrerahi vgvtpvassq dafhgsraai vslkeslcqe lgnfssqltf
      241 laftpivkss tpkeseeqva rnivravrtd qntlstgwvs rflyqtscav yygitlvtqw
      301 ihsqgcdyst
//
LOCUS       XP_076546048             310 aa            linear   INV 03-SEP-2025
DEFINITION  short-chain dehydrogenase/reductase family 16C member 6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546048
VERSION     XP_076546048.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689933.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="short-chain dehydrogenase/reductase family 16C
                     member 6"
                     /calculated_mol_wt=34448
     Region          61..283
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(66,68..69,71,90..92,162..164,203..205,213,217,
                     239..242)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187598"
     CDS             1..310
                     /gene="LOC117601492"
                     /coded_by="XM_076689933.1:593..1525"
                     /db_xref="GeneID:117601492"
ORIGIN      
        1 miysrdeali vnprmiypss tfwlfftvef ligallsafl aalallksll pkpprdltgd
       61 vvvvagassp lgkslaeefa kkgctvvcid sdsrsiqeta crlkatrpsv eeitprhrkd
      121 dssrhesmiy ayqcdlidrn dirrtaerik edigrvdilv tcvgnpnqdi fdatsrtlms
      181 hfwtamafvp lmlyrerahi vgvtpvassq dafhgsraai vslkeslcqe lgnfssqltf
      241 laftpivkss tpkeseeqva rnivravrtd qntlstgwvs rflyqtscav yygitlvtqw
      301 ihsqgcdyst
//
LOCUS       XP_076546050             310 aa            linear   INV 03-SEP-2025
DEFINITION  short-chain dehydrogenase/reductase family 16C member 6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546050
VERSION     XP_076546050.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689935.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="short-chain dehydrogenase/reductase family 16C
                     member 6"
                     /calculated_mol_wt=34448
     Region          61..283
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(66,68..69,71,90..92,162..164,203..205,213,217,
                     239..242)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187598"
     CDS             1..310
                     /gene="LOC117601492"
                     /coded_by="XM_076689935.1:574..1506"
                     /db_xref="GeneID:117601492"
ORIGIN      
        1 miysrdeali vnprmiypss tfwlfftvef ligallsafl aalallksll pkpprdltgd
       61 vvvvagassp lgkslaeefa kkgctvvcid sdsrsiqeta crlkatrpsv eeitprhrkd
      121 dssrhesmiy ayqcdlidrn dirrtaerik edigrvdilv tcvgnpnqdi fdatsrtlms
      181 hfwtamafvp lmlyrerahi vgvtpvassq dafhgsraai vslkeslcqe lgnfssqltf
      241 laftpivkss tpkeseeqva rnivravrtd qntlstgwvs rflyqtscav yygitlvtqw
      301 ihsqgcdyst
//
LOCUS       XP_076546051             310 aa            linear   INV 03-SEP-2025
DEFINITION  short-chain dehydrogenase/reductase family 16C member 6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546051
VERSION     XP_076546051.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689936.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="short-chain dehydrogenase/reductase family 16C
                     member 6"
                     /calculated_mol_wt=34448
     Region          61..283
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(66,68..69,71,90..92,162..164,203..205,213,217,
                     239..242)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187598"
     CDS             1..310
                     /gene="LOC117601492"
                     /coded_by="XM_076689936.1:708..1640"
                     /db_xref="GeneID:117601492"
ORIGIN      
        1 miysrdeali vnprmiypss tfwlfftvef ligallsafl aalallksll pkpprdltgd
       61 vvvvagassp lgkslaeefa kkgctvvcid sdsrsiqeta crlkatrpsv eeitprhrkd
      121 dssrhesmiy ayqcdlidrn dirrtaerik edigrvdilv tcvgnpnqdi fdatsrtlms
      181 hfwtamafvp lmlyrerahi vgvtpvassq dafhgsraai vslkeslcqe lgnfssqltf
      241 laftpivkss tpkeseeqva rnivravrtd qntlstgwvs rflyqtscav yygitlvtqw
      301 ihsqgcdyst
//
LOCUS       XP_076546052             310 aa            linear   INV 03-SEP-2025
DEFINITION  short-chain dehydrogenase/reductase family 16C member 6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546052
VERSION     XP_076546052.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689937.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="short-chain dehydrogenase/reductase family 16C
                     member 6"
                     /calculated_mol_wt=34448
     Region          61..283
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(66,68..69,71,90..92,162..164,203..205,213,217,
                     239..242)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187598"
     CDS             1..310
                     /gene="LOC117601492"
                     /coded_by="XM_076689937.1:744..1676"
                     /db_xref="GeneID:117601492"
ORIGIN      
        1 miysrdeali vnprmiypss tfwlfftvef ligallsafl aalallksll pkpprdltgd
       61 vvvvagassp lgkslaeefa kkgctvvcid sdsrsiqeta crlkatrpsv eeitprhrkd
      121 dssrhesmiy ayqcdlidrn dirrtaerik edigrvdilv tcvgnpnqdi fdatsrtlms
      181 hfwtamafvp lmlyrerahi vgvtpvassq dafhgsraai vslkeslcqe lgnfssqltf
      241 laftpivkss tpkeseeqva rnivravrtd qntlstgwvs rflyqtscav yygitlvtqw
      301 ihsqgcdyst
//
LOCUS       XP_034174164             310 aa            linear   INV 03-SEP-2025
DEFINITION  short-chain dehydrogenase/reductase family 16C member 6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174164
VERSION     XP_034174164.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318273.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174164.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="short-chain dehydrogenase/reductase family 16C
                     member 6"
                     /calculated_mol_wt=34448
     Region          61..283
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(66,68..69,71,90..92,162..164,203..205,213,217,
                     239..242)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187598"
     CDS             1..310
                     /gene="LOC117601492"
                     /coded_by="XM_034318273.2:1031..1963"
                     /db_xref="GeneID:117601492"
ORIGIN      
        1 miysrdeali vnprmiypss tfwlfftvef ligallsafl aalallksll pkpprdltgd
       61 vvvvagassp lgkslaeefa kkgctvvcid sdsrsiqeta crlkatrpsv eeitprhrkd
      121 dssrhesmiy ayqcdlidrn dirrtaerik edigrvdilv tcvgnpnqdi fdatsrtlms
      181 hfwtamafvp lmlyrerahi vgvtpvassq dafhgsraai vslkeslcqe lgnfssqltf
      241 laftpivkss tpkeseeqva rnivravrtd qntlstgwvs rflyqtscav yygitlvtqw
      301 ihsqgcdyst
//
LOCUS       XP_034174180             303 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601493 [Osmia lignaria lignaria].
ACCESSION   XP_034174180
VERSION     XP_034174180.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318289.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="uncharacterized protein LOC117601493"
                     /calculated_mol_wt=35229
     Region          33..>65
                     /region_name="ELM2"
                     /note="ELM2 domain; pfam01448"
                     /db_xref="CDD:460214"
     Region          136..179
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(136,166..167,169..170,172..174,176..178)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     CDS             1..303
                     /gene="LOC117601493"
                     /coded_by="XM_034318289.2:591..1502"
                     /db_xref="GeneID:117601493"
ORIGIN      
        1 mgihqnerkq kmdhsstdve rvernekcfd gvaeipefre qidvdreyqd fpeekiwtpq
       61 eevnewvpfy vfmarsvltf vqerrgisnr eamensctgy aeqyitdvlh dseysadtal
      121 rtlftkelpr klcarwtstd ielfikgitk hgknfsnirk dllpekdtkd lvdfyyawkw
      181 sepakklrnc rhqrrtkgkq rfpsiscifg glasskivtr svaaklsles keesneaiqn
      241 vllhditsit nsnedsvdkg sskirrhssk vlsniesper rrklkensns vgrkrrrngt
      301 fii
//
LOCUS       XP_034174191             303 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601493 [Osmia lignaria lignaria].
ACCESSION   XP_034174191
VERSION     XP_034174191.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318300.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="uncharacterized protein LOC117601493"
                     /calculated_mol_wt=35229
     Region          33..>65
                     /region_name="ELM2"
                     /note="ELM2 domain; pfam01448"
                     /db_xref="CDD:460214"
     Region          136..179
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(136,166..167,169..170,172..174,176..178)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     CDS             1..303
                     /gene="LOC117601493"
                     /coded_by="XM_034318300.2:656..1567"
                     /db_xref="GeneID:117601493"
ORIGIN      
        1 mgihqnerkq kmdhsstdve rvernekcfd gvaeipefre qidvdreyqd fpeekiwtpq
       61 eevnewvpfy vfmarsvltf vqerrgisnr eamensctgy aeqyitdvlh dseysadtal
      121 rtlftkelpr klcarwtstd ielfikgitk hgknfsnirk dllpekdtkd lvdfyyawkw
      181 sepakklrnc rhqrrtkgkq rfpsiscifg glasskivtr svaaklsles keesneaiqn
      241 vllhditsit nsnedsvdkg sskirrhssk vlsniesper rrklkensns vgrkrrrngt
      301 fii
//
LOCUS       XP_034174203             289 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule-associated protein RP/EB family member 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034174203
VERSION     XP_034174203.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318312.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="microtubule-associated protein RP/EB family
                     member 1 isoform X1"
                     /calculated_mol_wt=32748
     Region          16..279
                     /region_name="BIM1"
                     /note="Microtubule-binding protein involved in cell cycle
                     control [Cell division and chromosome partitioning /
                     Cytoskeleton]; COG5217"
                     /db_xref="CDD:227542"
     CDS             1..289
                     /gene="Eb1"
                     /coded_by="XM_034318312.2:387..1256"
                     /db_xref="GeneID:117601499"
ORIGIN      
        1 mavnvyatnv ttenlsrhdm lawvndclqs sftkieelct gavycqfmdm lfpgsvplkr
       61 vkfktnlehe yiqnfkilqg gfkkmnvdkv ipvdklvkgr fqdnfeflqw fkkffdanyd
      121 greydayear gcvplgsgvd gthnlsnpql vplppqskqm qmqqkhiqqr nivprqqqgi
      181 atsfnkapah rpqvktggig nrgdpgkvee lsaqlmelkm sleglekerd fyfgklrdie
      241 vmcqdcdnng dpppivqkil evlyateegf appeelegdg lapdeeeey
//
LOCUS       XP_034174212             289 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule-associated protein RP/EB family member 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034174212
VERSION     XP_034174212.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318321.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="microtubule-associated protein RP/EB family
                     member 1 isoform X1"
                     /calculated_mol_wt=32748
     Region          16..279
                     /region_name="BIM1"
                     /note="Microtubule-binding protein involved in cell cycle
                     control [Cell division and chromosome partitioning /
                     Cytoskeleton]; COG5217"
                     /db_xref="CDD:227542"
     CDS             1..289
                     /gene="Eb1"
                     /coded_by="XM_034318321.2:232..1101"
                     /db_xref="GeneID:117601499"
ORIGIN      
        1 mavnvyatnv ttenlsrhdm lawvndclqs sftkieelct gavycqfmdm lfpgsvplkr
       61 vkfktnlehe yiqnfkilqg gfkkmnvdkv ipvdklvkgr fqdnfeflqw fkkffdanyd
      121 greydayear gcvplgsgvd gthnlsnpql vplppqskqm qmqqkhiqqr nivprqqqgi
      181 atsfnkapah rpqvktggig nrgdpgkvee lsaqlmelkm sleglekerd fyfgklrdie
      241 vmcqdcdnng dpppivqkil evlyateegf appeelegdg lapdeeeey
//
LOCUS       XP_034174218             287 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule-associated protein RP/EB family member 1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034174218
VERSION     XP_034174218.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318327.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="microtubule-associated protein RP/EB family
                     member 1 isoform X2"
                     /calculated_mol_wt=31903
     Region          16..277
                     /region_name="BIM1"
                     /note="Microtubule-binding protein involved in cell cycle
                     control [Cell division and chromosome partitioning /
                     Cytoskeleton]; COG5217"
                     /db_xref="CDD:227542"
     CDS             1..287
                     /gene="Eb1"
                     /coded_by="XM_034318327.2:361..1224"
                     /db_xref="GeneID:117601499"
ORIGIN      
        1 mavnvyatnv ttenlsrhdm lawvndclqs sftkieelct gavycqfmdm lfpgsvplkr
       61 vkfktnlehe yiqnfkilqg gfkkmnvdki vpidrlvkgr fqdnfeflqw fkkffdanys
      121 raepydalam rggesmgsgg nnaphgtnak rttprdvnps kpagrigqgs sppapatnik
      181 sinkapahrp qvktggignr gdpgkveels aqlmelkmsl eglekerdfy fgklrdievm
      241 cqdcdnngdp ppivqkilev lyateegfap peelegdgla pdeeeey
//
LOCUS       XP_034174228             272 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule-associated protein RP/EB family member 1 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034174228
VERSION     XP_034174228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318337.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="microtubule-associated protein RP/EB family
                     member 1 isoform X3"
                     /calculated_mol_wt=30496
     Region          16..262
                     /region_name="BIM1"
                     /note="Microtubule-binding protein involved in cell cycle
                     control [Cell division and chromosome partitioning /
                     Cytoskeleton]; COG5217"
                     /db_xref="CDD:227542"
     CDS             1..272
                     /gene="Eb1"
                     /coded_by="XM_034318337.2:364..1182"
                     /db_xref="GeneID:117601499"
ORIGIN      
        1 mavnvyatnv ttenlsrhdm lawvndclqs sftkieelct gavycqfmdm lfpgsvplkr
       61 vkfktnlehe yiqnfkilqg gfkkmnvdki vpidrlvkgr fqdnfeflqw fkkffdanys
      121 raepydalam rggesmgsgg nnaphgtnak rttprdvnps kpagrivnka pahrpqvktg
      181 gignrgdpgk veelsaqlme lkmsleglek erdfyfgklr dievmcqdcd nngdpppivq
      241 kilevlyate egfappeele gdglapdeee ey
//
LOCUS       XP_034174269             123 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601528 [Osmia lignaria lignaria].
ACCESSION   XP_034174269
VERSION     XP_034174269.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318378.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="uncharacterized protein LOC117601528"
                     /calculated_mol_wt=14854
     Region          1..120
                     /region_name="OTU"
                     /note="OTU (ovarian tumor) domain family; cl45892"
                     /db_xref="CDD:459237"
     Site            order(50,53,60..63,87,89,108,115..117)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:438581"
     CDS             1..123
                     /gene="LOC117601528"
                     /coded_by="XM_034318378.2:63..434"
                     /db_xref="GeneID:117601528"
ORIGIN      
        1 mfraisyilw rnedehrylr tmvvqhiken wqeygpfvma ewnisdrqty cdymnmvgtf
       61 aselectvat kmyymnlsiy reinerqelk rvfhnhvssr yetarllftg nsdnghydvl
      121 fpe
//
LOCUS       XP_076546053             790 aa            linear   INV 03-SEP-2025
DEFINITION  4'-phosphopantetheine phosphatase [Osmia lignaria lignaria].
ACCESSION   XP_076546053
VERSION     XP_076546053.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689938.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..790
                     /product="4'-phosphopantetheine phosphatase"
                     /calculated_mol_wt=88529
     Region          34..758
                     /region_name="PLN02902"
                     /note="pantothenate kinase"
                     /db_xref="CDD:215489"
     CDS             1..790
                     /gene="LOC117611971"
                     /coded_by="XM_076689938.1:247..2619"
                     /db_xref="GeneID:117611971"
ORIGIN      
        1 metaeieder thirpiklpa avevfanlkn aqkfaidigl sltkiayyst vsyrkalyed
       61 tglgnsgera ykvhegnrlh fvkfetryie ncldfvkenl vnverfqgks ikvtgggayk
      121 ytpllqeklg ltvdkedemt clikgcnfll knisceafef erhgnpeykf qkaglnifpy
      181 mlvnigsgvs ilkvesdqvf ervggtatgg gtfwglgsll tkrkgfdell qlaergdhrn
      241 vdmlvkdiyg gdyssqglpg dliaasfgkv vsykgqpkfs eadlarslll tisndigqia
      301 slyatvhkmd kvyfggyflr nhplsmhtis ysikywsngk vkplflrheg ylgaigafly
      361 gaeqsnehsw lenyagssgf kdsistnlgi kvdqleidqa enavtfcpll kdpssynpdt
      421 tdlaqdkear dywlqcfees vdkfvaraih sqphsptakd ratklkekyv nrlhylhlqp
      481 faygtltvrv lldtiehcmk efdfpdpylh qkkqeneeal khlknriiai dylegadkik
      541 alilgvlsgn mfdwganava tlmettdfgf aeaqakipgr pwlqddlddw idrletgpph
      601 kcaaifvdns gvdivlgilp fardlllrgt kvilcansmp alndvtypel vvtlrdaaki
      661 ckvirqalke nrliametaq agpcldlsrl nldlclamvk hnvdlivleg mgrtlhtnlh
      721 akltceclkl avvknrwlal rlggdmyavi ckyerppqei kiydiemkpt ecppecvetv
      781 teictcdikk
//
LOCUS       XP_034196424             790 aa            linear   INV 03-SEP-2025
DEFINITION  4'-phosphopantetheine phosphatase [Osmia lignaria lignaria].
ACCESSION   XP_034196424
VERSION     XP_034196424.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340533.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196424.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..790
                     /product="4'-phosphopantetheine phosphatase"
                     /calculated_mol_wt=88529
     Region          34..758
                     /region_name="PLN02902"
                     /note="pantothenate kinase"
                     /db_xref="CDD:215489"
     CDS             1..790
                     /gene="LOC117611971"
                     /coded_by="XM_034340533.2:247..2619"
                     /db_xref="GeneID:117611971"
ORIGIN      
        1 metaeieder thirpiklpa avevfanlkn aqkfaidigl sltkiayyst vsyrkalyed
       61 tglgnsgera ykvhegnrlh fvkfetryie ncldfvkenl vnverfqgks ikvtgggayk
      121 ytpllqeklg ltvdkedemt clikgcnfll knisceafef erhgnpeykf qkaglnifpy
      181 mlvnigsgvs ilkvesdqvf ervggtatgg gtfwglgsll tkrkgfdell qlaergdhrn
      241 vdmlvkdiyg gdyssqglpg dliaasfgkv vsykgqpkfs eadlarslll tisndigqia
      301 slyatvhkmd kvyfggyflr nhplsmhtis ysikywsngk vkplflrheg ylgaigafly
      361 gaeqsnehsw lenyagssgf kdsistnlgi kvdqleidqa enavtfcpll kdpssynpdt
      421 tdlaqdkear dywlqcfees vdkfvaraih sqphsptakd ratklkekyv nrlhylhlqp
      481 faygtltvrv lldtiehcmk efdfpdpylh qkkqeneeal khlknriiai dylegadkik
      541 alilgvlsgn mfdwganava tlmettdfgf aeaqakipgr pwlqddlddw idrletgpph
      601 kcaaifvdns gvdivlgilp fardlllrgt kvilcansmp alndvtypel vvtlrdaaki
      661 ckvirqalke nrliametaq agpcldlsrl nldlclamvk hnvdlivleg mgrtlhtnlh
      721 akltceclkl avvknrwlal rlggdmyavi ckyerppqei kiydiemkpt ecppecvetv
      781 teictcdikk
//
LOCUS       XP_034195347             285 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611482 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195347
VERSION     XP_034195347.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339456.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="uncharacterized protein LOC117611482 isoform X1"
                     /calculated_mol_wt=33020
     Region          160..284
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(254,258,260,268,270..273,276..278)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..285
                     /gene="LOC117611482"
                     /coded_by="XM_034339456.2:16..873"
                     /db_xref="GeneID:117611482"
ORIGIN      
        1 mikvkmgrcr vgfvvwlvlt cftsdalqqy reknisgaet davqailary lqrrlqgsql
       61 stppppavff ssnnqarvrq apqrttgnal prndsekenr epvedyedye ddlegfedse
      121 rsrtrfdlfa ddcpncvdeh ssslaasrwt mpllklgekr yylgiffkan wyrasqycry
      181 hgmhlasias qeendrlekh ikdfglgheh fwtsgtdqae egtffwmang rpitfenwnv
      241 gepnnfryen geeehclelw nrdgkglkwn dspcsfetff vcevq
//
LOCUS       XP_034195373             283 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611482 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195373
VERSION     XP_034195373.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339482.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="uncharacterized protein LOC117611482 isoform X2"
                     /calculated_mol_wt=32778
     Region          158..282
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(252,256,258,266,268..271,274..276)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..283
                     /gene="LOC117611482"
                     /coded_by="XM_034339482.2:402..1253"
                     /db_xref="GeneID:117611482"
ORIGIN      
        1 mvkmgrcrvg fvvwlvltcf tsdalqqyre knisgaetda vqailarylq rrlqgsqlst
       61 ppppavffss nnqarvrqap qrttgnalpr ndsekenrep vedyedyedd legfedsers
      121 rtrfdlfadd cpncvdehss slaasrwtmp llklgekryy lgiffkanwy rasqycryhg
      181 mhlasiasqe endrlekhik dfglghehfw tsgtdqaeeg tffwmangrp itfenwnvge
      241 pnnfryenge eehclelwnr dgkglkwnds pcsfetffvc evq
//
LOCUS       XP_034195393             283 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611482 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195393
VERSION     XP_034195393.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339502.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="uncharacterized protein LOC117611482 isoform X2"
                     /calculated_mol_wt=32778
     Region          158..282
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(252,256,258,266,268..271,274..276)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..283
                     /gene="LOC117611482"
                     /coded_by="XM_034339502.2:36..887"
                     /db_xref="GeneID:117611482"
ORIGIN      
        1 mvkmgrcrvg fvvwlvltcf tsdalqqyre knisgaetda vqailarylq rrlqgsqlst
       61 ppppavffss nnqarvrqap qrttgnalpr ndsekenrep vedyedyedd legfedsers
      121 rtrfdlfadd cpncvdehss slaasrwtmp llklgekryy lgiffkanwy rasqycryhg
      181 mhlasiasqe endrlekhik dfglghehfw tsgtdqaeeg tffwmangrp itfenwnvge
      241 pnnfryenge eehclelwnr dgkglkwnds pcsfetffvc evq
//
LOCUS       XP_034196465             622 aa            linear   INV 03-SEP-2025
DEFINITION  protein arginine N-methyltransferase 5 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196465
VERSION     XP_034196465.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..622
                     /product="protein arginine N-methyltransferase 5 isoform
                     X1"
                     /calculated_mol_wt=70874
     Region          30..274
                     /region_name="PRMT5_TIM"
                     /note="PRMT5 TIM barrel domain; pfam17285"
                     /db_xref="CDD:435840"
     Region          281..449
                     /region_name="PRMT5"
                     /note="PRMT5 arginine-N-methyltransferase; pfam05185"
                     /db_xref="CDD:428356"
     Region          452..620
                     /region_name="PRMT5_C"
                     /note="PRMT5 oligomerization domain; pfam17286"
                     /db_xref="CDD:435841"
     CDS             1..622
                     /gene="csul"
                     /coded_by="XM_034340574.2:260..2128"
                     /db_xref="GeneID:117611993"
ORIGIN      
        1 mtnqrpvscg ldfcavpdls qclftanssk yeficaplvh plykrefvsg vaknrtgpft
       61 rpdlvlcssd wnnliigkls phikvdsknp vlrknseetl iqeltlashl glvgvtfklt
      121 rgiehnanla riicdkvssp cglqvwiqvp menpikqahs yreeeydsve spwewwndfr
      181 aicdydrklg valivshdlp eqeeidkwlg epvkclifpt tsfitnkkgf pvlskahqal
      241 vkkfsmlevq fvltganrhq nisyyhdyle ylwkgcqmgg pverfargye dylqcplqpl
      301 mdnlesqtye ifekdpvkyt qyqtaiyqai lkimsteedk srkiiimvvg agrgplvhas
      361 lnaaemanqq ikvyaveknp navltlqale rdvwedkvtv vscdmrdwka peeadilvse
      421 llgsfgdnel specldgvqr flkddgisip csytsyiapv qssklfnevk qckdkdkhpl
      481 ahfetsyvvh lqnkydiakp qplftfrhpn kdsvidnsry eiktfevqqn svlhgfsgyf
      541 dtvlfkdvtl siqpstrspg mfswfpiffp ikepvqlkag dqivvhfwrq cssknvwyew
      601 ciskpiavpv hnpngrsyti gl
//
LOCUS       XP_034196492             554 aa            linear   INV 03-SEP-2025
DEFINITION  protein arginine N-methyltransferase 5 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196492
VERSION     XP_034196492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..554
                     /product="protein arginine N-methyltransferase 5 isoform
                     X2"
                     /calculated_mol_wt=63028
     Region          30..274
                     /region_name="PRMT5_TIM"
                     /note="PRMT5 TIM barrel domain; pfam17285"
                     /db_xref="CDD:435840"
     Region          281..447
                     /region_name="PRMT5"
                     /note="PRMT5 arginine-N-methyltransferase; pfam05185"
                     /db_xref="CDD:428356"
     Region          <447..552
                     /region_name="PRMT5_C"
                     /note="PRMT5 oligomerization domain; pfam17286"
                     /db_xref="CDD:435841"
     CDS             1..554
                     /gene="csul"
                     /coded_by="XM_034340601.2:268..1932"
                     /db_xref="GeneID:117611993"
ORIGIN      
        1 mtnqrpvscg ldfcavpdls qclftanssk yeficaplvh plykrefvsg vaknrtgpft
       61 rpdlvlcssd wnnliigkls phikvdsknp vlrknseetl iqeltlashl glvgvtfklt
      121 rgiehnanla riicdkvssp cglqvwiqvp menpikqahs yreeeydsve spwewwndfr
      181 aicdydrklg valivshdlp eqeeidkwlg epvkclifpt tsfitnkkgf pvlskahqal
      241 vkkfsmlevq fvltganrhq nisyyhdyle ylwkgcqmgg pverfargye dylqcplqpl
      301 mdnlesqtye ifekdpvkyt qyqtaiyqai lkimsteedk srkiiimvvg agrgplvhas
      361 lnaaemanqq ikvyaveknp navltlqale rdvwedkvtv vscdmrdwka peeadilvse
      421 llgsfgdnel specldgvqr flkdsvidns ryeiktfevq qnsvlhgfsg yfdtvlfkdv
      481 tlsiqpstrs pgmfswfpif fpikepvqlk agdqivvhfw rqcssknvwy ewciskpiav
      541 pvhnpngrsy tigl
//
LOCUS       XP_034196505             200 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612009 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196505
VERSION     XP_034196505.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340614.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..200
                     /product="uncharacterized protein LOC117612009 isoform X1"
                     /calculated_mol_wt=24097
     CDS             1..200
                     /gene="LOC117612009"
                     /coded_by="XM_034340614.2:22..624"
                     /db_xref="GeneID:117612009"
ORIGIN      
        1 mdvilsfkdk hvkvsnsqik dllqkqikli devkqkqkyw neneikiqel tikvtsmken
       61 lqcekqaley kdhdlskhld fiaklkaekm kfleenqlle lqrnklknck rnlkdqklld
      121 qgrrkytlyk elsgirwdfe tfkenvrgyv knqkkyihhf syknenvqnl nkllwqeiyq
      181 siieaenkeh dkesvqinav
//
LOCUS       XP_034196541             165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612009 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196541
VERSION     XP_034196541.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340650.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..165
                     /product="uncharacterized protein LOC117612009 isoform X2"
                     /calculated_mol_wt=19884
     CDS             1..165
                     /gene="LOC117612009"
                     /coded_by="XM_034340650.2:80..577"
                     /db_xref="GeneID:117612009"
ORIGIN      
        1 mtfsflenei kiqeltikvt smkenlqcek qaleykdhdl skhldfiakl kaekmkflee
       61 nqllelqrnk lknckrnlkd qklldqgrrk ytlykelsgi rwdfetfken vrgyvknqkk
      121 yihhfsykne nvqnlnkllw qeiyqsiiea enkehdkesv qinav
//
LOCUS       XP_034196555             172 aa            linear   INV 03-SEP-2025
DEFINITION  glycine cleavage system H protein, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034196555
VERSION     XP_034196555.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340664.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..172
                     /product="glycine cleavage system H protein,
                     mitochondrial"
                     /calculated_mol_wt=19261
     Region          46..163
                     /region_name="GcvH"
                     /note="Glycine cleavage system protein H (lipoate-binding)
                     [Amino acid transport and metabolism]; COG0509"
                     /db_xref="CDD:440275"
     CDS             1..172
                     /gene="ppl"
                     /coded_by="XM_034340664.2:159..677"
                     /db_xref="GeneID:117612029"
ORIGIN      
        1 makviarmtk ytartfpcvs renlftltst kvsfnvsrsi attrclradr lftkrhewii
       61 idgkigtvgi snyaqeslgd vvyaqlpdve neiekeaecg alesvkaase lyspvsgkvi
      121 ekneavektp slinsscyek gwlfkvelkn pdeikdlmne esynlylksd ph
//
LOCUS       XP_034171836             123 aa            linear   INV 03-SEP-2025
DEFINITION  histone H2B [Osmia lignaria lignaria].
ACCESSION   XP_034171836
VERSION     XP_034171836.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315945.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="histone H2B"
                     /calculated_mol_wt=13563
     Region          33..120
                     /region_name="HFD_H2B"
                     /note="histone-fold domain found in histone H2B and
                     similar proteins; cd22910"
                     /db_xref="CDD:467035"
     Site            order(33,35,37..39,41..43,46..53,55..56,59..60,63..64,
                     66..68,70..72,76,86..87,89,92..93,96,99..101,104,107..109,
                     111..112,115..116,119..120)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467035"
     Site            order(36..38,40..41,44..46,51..53,57,93,97,106..107,
                     109..110,113..114,116..117,120)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467035"
     Site            order(59,62..63,65..66,69,74,77,80..82,90..91,94,98..99)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467035"
     CDS             1..123
                     /gene="LOC117600453"
                     /coded_by="XM_034315945.2:44..415"
                     /db_xref="GeneID:117600453"
ORIGIN      
        1 mppkasgkav kkagkaqkni sktdkkkkrr rkesyaiyiy kvlkqvhpdt gisskamsim
       61 nsfvndvfer iaaeasrlah ynkrstitsr eiqtavrlll pgelakhavs egtkavtkyt
      121 ssk
//
LOCUS       XP_034194790             338 aa            linear   INV 03-SEP-2025
DEFINITION  trypsin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034194790
VERSION     XP_034194790.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338899.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194790.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="trypsin-1"
                     /calculated_mol_wt=37062
     Region          101..331
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            101
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(141,190,285)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(279,304,306)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..338
                     /gene="LOC117611012"
                     /coded_by="XM_034338899.2:349..1365"
                     /db_xref="GeneID:117611012"
ORIGIN      
        1 mcirsvlcvv ivitttcfvn cgelisgvdv vnlnstkndv pnntqngans taitddknfw
       61 ewltgfisag npvttteppt pvkphsclpc kcglantqrr ivggvetqvn qypwmalmtf
      121 rgrfycgasv issryvltaa hcvdrfdpnl mtiqilehdr nsttesetqv fkvdkvikhi
      181 gystfnynnd ialvklkgii rfegkmrpvc lperaktfag tdgivtgwga ieesgpisqt
      241 lqevvvpilt ntecratkyp arritdnmlc agykegqkds cqgdsggplh vvinnihsiv
      301 gvvswgegca kpgypgvysr vnryitwieq ntedacyc
//
LOCUS       XP_076546054             173 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305638 [Osmia lignaria lignaria].
ACCESSION   XP_076546054
VERSION     XP_076546054.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689939.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..173
                     /product="uncharacterized protein LOC143305638"
                     /calculated_mol_wt=19668
     Region          17..114
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     CDS             1..173
                     /gene="LOC143305638"
                     /coded_by="XM_076689939.1:1076..1597"
                     /db_xref="GeneID:143305638"
ORIGIN      
        1 mntiknivls ssvrigivhv dnpvefwaqv ddgreearrl yrdlyrymsd rghlhfpnqg
       61 rlttgrlvay rskegtwvrg evqhlvdgqf evalrdhgkv afkvrvrgir pvcrtwkieq
      121 akrvlegrrg lltnirgege vvadvaldrl pgqppfnvae awlgmryavp grv
//
LOCUS       XP_034196568             704 aa            linear   INV 03-SEP-2025
DEFINITION  arginine methyltransferase 7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196568
VERSION     XP_034196568.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340677.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196568.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..704
                     /product="arginine methyltransferase 7 isoform X1"
                     /calculated_mol_wt=80025
     Region          52..>207
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     Region          406..>488
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     CDS             1..704
                     /gene="Art7"
                     /coded_by="XM_034340677.2:332..2446"
                     /db_xref="GeneID:117612036"
ORIGIN      
        1 mlkifftrfi hsrnmsiftq clnpltgtft weekdenydy hqevarsafa dmlhdhernq
       61 kyyealktai ekkhqmgeea nvldigtgtg llsmmsakcg adtitaceaf tpmakcaiki
      121 ieengfedki klvhkrstkm tigkdgdmak ranilvtevf dteligegal stfrhahenl
      181 leensiviph tatiwaqvve sstvcawntv hsiqhnnkrl lniphavkcc sgaaavhdiq
      241 lsqfphnafk pllpiqpifr fdfsskapil fhdkvclhvk piasgtarai fmwwdlnmdi
      301 dnkvllscap vwkhpdakil qekgltleki tdlipwrdhw mqaiyylple tpvtvnnevs
      361 ligyhdeysl wfqlinesin eipdcerpmc tcsihvaycr trigqlndqk rndkyiqale
      421 kkvtsdtvcl clsdgcllgl aaiklgakki filetnflsk kcmemfikan dltkkvqiip
      481 svdelppenm inlifgepyf itsivpwenl yfwylisrys sqiqripigm timgvvvefk
      541 dlhkiratlg icegfdlsif dnliqtssdk cdspieaqpl weypgkalsl pfviqkfdlt
      601 gnvtehedin ilnavpilet gmcngvalwv dwdldseitv ssgptakiqp gkriswdpft
      661 rqgvhlfrdi snvtqqnmll wsfnfipqhg nikfdfrvls knvk
//
LOCUS       XP_034196578             592 aa            linear   INV 03-SEP-2025
DEFINITION  arginine methyltransferase 7 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196578
VERSION     XP_034196578.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196578.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..592
                     /product="arginine methyltransferase 7 isoform X2"
                     /calculated_mol_wt=67209
     Region          <1..>95
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     Region          294..>376
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     CDS             1..592
                     /gene="Art7"
                     /coded_by="XM_034340687.2:179..1957"
                     /db_xref="GeneID:117612036"
ORIGIN      
        1 makcaikiie engfedkikl vhkrstkmti gkdgdmakra nilvtevfdt eligegalst
       61 frhahenlle ensiviphta tiwaqvvess tvcawntvhs iqhnnkrlln iphavkccsg
      121 aaavhdiqls qfphnafkpl lpiqpifrfd fsskapilfh dkvclhvkpi asgtaraifm
      181 wwdlnmdidn kvllscapvw khpdakilqe kgltlekitd lipwrdhwmq aiyylpletp
      241 vtvnnevsli gyhdeyslwf qlinesinei pdcerpmctc sihvaycrtr igqlndqkrn
      301 dkyiqalekk vtsdtvclcl sdgcllglaa iklgakkifi letnflskkc memfikandl
      361 tkkvqiipsv delppenmin lifgepyfit sivpwenlyf wylisryssq iqripigmti
      421 mgvvvefkdl hkiratlgic egfdlsifdn liqtssdkcd spieaqplwe ypgkalslpf
      481 viqkfdltgn vtehedinil navpiletgm cngvalwvdw dldseitvss gptakiqpgk
      541 riswdpftrq gvhlfrdisn vtqqnmllws fnfipqhgni kfdfrvlskn vk
//
LOCUS       XP_034196589             592 aa            linear   INV 03-SEP-2025
DEFINITION  arginine methyltransferase 7 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196589
VERSION     XP_034196589.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340698.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196589.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..592
                     /product="arginine methyltransferase 7 isoform X2"
                     /calculated_mol_wt=67209
     Region          <1..>95
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     Region          294..>376
                     /region_name="COG4076"
                     /note="Predicted RNA methylase [General function
                     prediction only]"
                     /db_xref="CDD:443253"
     CDS             1..592
                     /gene="Art7"
                     /coded_by="XM_034340698.2:163..1941"
                     /db_xref="GeneID:117612036"
ORIGIN      
        1 makcaikiie engfedkikl vhkrstkmti gkdgdmakra nilvtevfdt eligegalst
       61 frhahenlle ensiviphta tiwaqvvess tvcawntvhs iqhnnkrlln iphavkccsg
      121 aaavhdiqls qfphnafkpl lpiqpifrfd fsskapilfh dkvclhvkpi asgtaraifm
      181 wwdlnmdidn kvllscapvw khpdakilqe kgltlekitd lipwrdhwmq aiyylpletp
      241 vtvnnevsli gyhdeyslwf qlinesinei pdcerpmctc sihvaycrtr igqlndqkrn
      301 dkyiqalekk vtsdtvclcl sdgcllglaa iklgakkifi letnflskkc memfikandl
      361 tkkvqiipsv delppenmin lifgepyfit sivpwenlyf wylisryssq iqripigmti
      421 mgvvvefkdl hkiratlgic egfdlsifdn liqtssdkcd spieaqplwe ypgkalslpf
      481 viqkfdltgn vtehedinil navpiletgm cngvalwvdw dldseitvss gptakiqpgk
      541 riswdpftrq gvhlfrdisn vtqqnmllws fnfipqhgni kfdfrvlskn vk
//
LOCUS       XP_034196602             252 aa            linear   INV 03-SEP-2025
DEFINITION  DTW domain containing 2 [Osmia lignaria lignaria].
ACCESSION   XP_034196602
VERSION     XP_034196602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..252
                     /product="DTW domain containing 2"
                     /calculated_mol_wt=27879
     Region          21..213
                     /region_name="DTW"
                     /note="DTW domain; pfam03942"
                     /db_xref="CDD:461101"
     CDS             1..252
                     /gene="Dtwd2"
                     /coded_by="XM_034340711.2:305..1063"
                     /db_xref="GeneID:117612045"
ORIGIN      
        1 mnetsvlqel seiladppet rdkctqckrp vavcwcpglp khpvcpasri iilqhpaevk
       61 rclrtapmla lglesgkcli frgkkfplpk heglaeilnd rntlllypsp gaiqldelhp
      121 vgvngqkpyn lvlldgtwpq akaiyhsspa lcflqacklv gvptseyvir tqptegclst
      181 letgafalsi lendpelkdk mlgplhylcr fqlengavth qskeflikqk aypkligrrl
      241 akqlrmlpde st
//
LOCUS       XP_034196617             627 aa            linear   INV 03-SEP-2025
DEFINITION  signal recognition particle receptor alpha isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196617
VERSION     XP_034196617.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340726.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="signal recognition particle receptor alpha
                     isoform X1"
                     /calculated_mol_wt=70761
     Region          29..280
                     /region_name="SRP-alpha_N"
                     /note="Signal recognition particle, alpha subunit,
                     N-terminal; pfam04086"
                     /db_xref="CDD:461165"
     Region          307..379
                     /region_name="SRP54_N"
                     /note="SRP54-type protein, helical bundle domain;
                     pfam02881"
                     /db_xref="CDD:460734"
     Region          409..625
                     /region_name="SRalpha_C"
                     /note="C-terminal domain of signal recognition particle
                     receptor alpha subunit; cd17876"
                     /db_xref="CDD:349785"
     Site            order(416..423,427,445,448,454,513,579,581,605..608)
                     /site_type="active"
                     /db_xref="CDD:349785"
     Site            order(417..418,447..450,453..454,457..458,484,513,
                     515..517,542,544..548,551,579,581..582,584,608)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:349785"
     CDS             1..627
                     /gene="SrpRalpha"
                     /coded_by="XM_034340726.2:128..2011"
                     /db_xref="GeneID:117612050"
ORIGIN      
        1 mldlftifsk ggivlwcfqs tsqifapsvn alirsvilqe rtgnhtfeyd slrlqykldn
       61 efelvfvvay qkilqlsyid kflndihlef rdrfknelek sqwfynyefd knyehvlama
      121 eqwartqaki pkqmrtfdes lkskktvasm ierkddkdnk kqgkrkkgvn tnkdvqlevq
      181 evpkqelpnn qtnhngeine eilianrvrl aqklnnqkkk vdkpksqkpe kagkkprvwe
      241 lggttkdlat lertkdkpee sgdyvpadtt lvgqmkgsir dieiesesed esdqeiensk
      301 pqvqkktnsm fsmfkslvgn kslkhedmap vldklkdhli gknvaaeiaq klcdsvgvkl
      361 egkvlgtfds vsstvkatlt dslvqilspk rrvdilrdam eakrsnrpyv mtfcgvngvg
      421 kstnlakicf wliennfrvl iaacdtfrag aveqlrthmr hlnalhppek hgnqsmvqly
      481 ekgygkdaag iameairfar dsrfdvvlvd tagrmqdnep lmralaklik vnepdlvlfv
      541 gealvgneav dqlvkfnqal adhsqstnph iidgivltkf dtiddkvgaa ismtyitgqp
      601 ivfvgtgqty tdlkslnaka vvhalmk
//
LOCUS       XP_034196625             613 aa            linear   INV 03-SEP-2025
DEFINITION  signal recognition particle receptor alpha isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196625
VERSION     XP_034196625.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340734.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..613
                     /product="signal recognition particle receptor alpha
                     isoform X2"
                     /calculated_mol_wt=69194
     Region          29..266
                     /region_name="SRP-alpha_N"
                     /note="Signal recognition particle, alpha subunit,
                     N-terminal; pfam04086"
                     /db_xref="CDD:461165"
     Region          293..365
                     /region_name="SRP54_N"
                     /note="SRP54-type protein, helical bundle domain;
                     pfam02881"
                     /db_xref="CDD:460734"
     Region          395..611
                     /region_name="SRalpha_C"
                     /note="C-terminal domain of signal recognition particle
                     receptor alpha subunit; cd17876"
                     /db_xref="CDD:349785"
     Site            order(402..409,413,431,434,440,499,565,567,591..594)
                     /site_type="active"
                     /db_xref="CDD:349785"
     Site            order(403..404,433..436,439..440,443..444,470,499,
                     501..503,528,530..534,537,565,567..568,570,594)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:349785"
     CDS             1..613
                     /gene="SrpRalpha"
                     /coded_by="XM_034340734.2:129..1970"
                     /db_xref="GeneID:117612050"
ORIGIN      
        1 mldlftifsk ggivlwcfqs tsqifapsvn alirsvilqe rtgnhtfeyd slrlqykldn
       61 efelvfvvay qkilqlsyid kflndihlef rdrfknelek sqwfynyefd knyehvlama
      121 eqwartqaki pkqmrtfdes lkskktvasm ierkddkdnk kqvevqevpk qelpnnqtnh
      181 ngeineeili anrvrlaqkl nnqkkkvdkp ksqkpekagk kprvwelggt tkdlatlert
      241 kdkpeesgdy vpadttlvgq mkgsirdiei esesedesdq eienskpqvq kktnsmfsmf
      301 kslvgnkslk hedmapvldk lkdhligknv aaeiaqklcd svgvklegkv lgtfdsvsst
      361 vkatltdslv qilspkrrvd ilrdameakr snrpyvmtfc gvngvgkstn lakicfwlie
      421 nnfrvliaac dtfragaveq lrthmrhlna lhppekhgnq smvqlyekgy gkdaagiame
      481 airfardsrf dvvlvdtagr mqdneplmra laklikvnep dlvlfvgeal vgneavdqlv
      541 kfnqaladhs qstnphiidg ivltkfdtid dkvgaaismt yitgqpivfv gtgqtytdlk
      601 slnakavvha lmk
//
LOCUS       XP_076546056             124 aa            linear   INV 03-SEP-2025
DEFINITION  histone H2A-like [Osmia lignaria lignaria].
ACCESSION   XP_076546056
VERSION     XP_076546056.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689941.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="histone H2A-like"
                     /calculated_mol_wt=13162
     Region          16..124
                     /region_name="PTZ00017"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:185399"
     CDS             1..124
                     /gene="LOC117600390"
                     /coded_by="XM_076689941.1:39..413"
                     /db_xref="GeneID:117600390"
ORIGIN      
        1 msgrgkggka kgkaksrssr aglqfpvgri hrllrkgnya ervgagapvy laavmeylaa
       61 evlelagnaa rdnkktriip rhlqlairnd eelnkllsgv tiaqggvlpn iqavllpkkt
      121 dkka
//
LOCUS       XP_034170934             199 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600054 [Osmia lignaria lignaria].
ACCESSION   XP_034170934
VERSION     XP_034170934.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315043.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034170934.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="uncharacterized protein LOC117600054"
                     /calculated_mol_wt=23619
     Region          13..151
                     /region_name="CK2S"
                     /note="Casein Kinase 2 substrate; pfam15011"
                     /db_xref="CDD:464443"
     CDS             1..199
                     /gene="LOC117600054"
                     /coded_by="XM_034315043.2:76..675"
                     /db_xref="GeneID:117600054"
ORIGIN      
        1 makdlhkcli nyfsqlekvd ckwkelseea erpiqalrnq seqlrlvtsk evndaelckm
       61 devherlifk ilmginnelv lifdiltrfn ninqdlknrl knledarska slneetmkel
      121 ingspyrprl nlllewaida fnhyhelyll ingnlrkcdy kdenmiedlr ksfvedrfkr
      181 srmdrilgst qfliketvr
//
LOCUS       XP_034170855            2194 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034170855
VERSION     XP_034170855.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034314964.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034170855.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2194
                     /product="disco-interacting protein 2 isoform X1"
                     /calculated_mol_wt=239082
     Region          <565..>582
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          949..1523
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1108,1111..1116,1118..1119)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1111,1231..1232,1290..1295,1400,1403,1414,1503)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1111,1152..1153,1203,1205..1206,1209,1231..1232,
                     1250..1255,1400,1403,1411..1414,1484)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1152,1205..1206,1209,1231,1411..1413,1471,1484)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1606..2190
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1755,1758..1763,1765..1766)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1758,1884..1885,1939..1944,2035,2077,2080,2091,2170)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1758,1799..1800,1850,1852..1853,1856,1884..1885,
                     1902..1907,2035,2077,2080,2088..2091,2151)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1799,1852..1853,1856,1884,2088..2090,2138,2151)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2194
                     /gene="DIP2"
                     /coded_by="XM_034314964.2:3575..10159"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsggrlvpgg iasppgsggs tgntgnsnsa aarrgnrrlt rnesryhsev rqeavqqala
      721 amqgrpkpsl pmpskrtsvm arspdrerrd sgesssdeds vvteespgag gptgtglsdt
      781 sstgsardtp ppprpparrp pgaditdiae yaphaycniq ppdvthtsnt psaqqstrrp
      841 gadrvnryhv vedqnntgtt grwkvsakiq qllntlkrpk rrplpefyed ddieleiaan
      901 pkdpnapkpe ggsmtsavge plsvaaglpr sleaaiqryg sasykapvat vldpngklcv
      961 tltygkllsr shkiaytlln kalsrggdcc lkpgdrialv ypnndpisfm cafygclqag
     1021 ivpvpievpl trrdagsqqi gfllgscgiq valtseaclk glpktaagev vafkgwpklh
     1081 wfvtehlgkt pkdwlppprl tddtpayiey ttdkdgsvmg vtvtrsamla hcraltqacg
     1141 ytegenavcv ldfkrevglw hstltsilng mhvifipyal mkvnpaswmq mitkhrasva
     1201 vvksrdlhwg llatkdhkdi slsslrlllv adganpwsls scdqflsvfq skglrpdavc
     1261 pcasssealt vsvrrpgrag vnatgrgvls msglsygvvr vdqensltsl tlqdcgqvmp
     1321 gsivvvikme gqpficktde vgeicvhssa tgnqywglqg ltnntfkvsp lqadgtplgd
     1381 veytrsgllg flgpgglvfv cgsrdglmtv tgrkhnaddi iatvlavepm kfiyrgriav
     1441 fsvrvlrder icvvaeqrpd cseeesfqwm srvlqavdsi havgiyclal vppnhlpktp
     1501 lggihlsetk rrflegtlhp anvllcphtc vtnlpkprev hsagdsvadv gpasvmvgni
     1561 vqgnrlasaq grdmgvlded sdnakkyqfi seilrwravs tsdhviftsl nakgavatsl
     1621 scsqlhkkae rignllldrg rintgdhval ifppgtdlic afygclyvga vpvtirpphp
     1681 qnlqttlptv rmivdvsksv lvltnqnilk llktkeannv vdikswptil dmddmpkkkl
     1741 pvmyraptae mlayldfsvs ttgmlagikm shaavtslcr amklacelyp srhialcldp
     1801 ysglgfalwc lssiysghhs ilippsevea npalwlsavs qsrvrdtfcs ygvmelctkg
     1861 lgssvhalka rgvslacvrt cvvvaeerpr ialttsfskl fsalglspra vstsfgcrvn
     1921 taiclqgass pepstvyvdl ralrndrvsl vergsphslc lmesgkllpg vkviianpet
     1981 kgqcgdshlg eiwvqsahna sgyftiygde sdyadhfnar lvtgntnevy artgylgflr
     2041 rtesvqqsvi sdvpgdtsaa eadlvpgdae lhdavfvvga ldeaillrgm ryhpidiens
     2101 vmrchkkiae cavftwtnll vvvveldgse sealdlvalv tsavleehhl vvgvvvvvdp
     2161 gvvpinsrge kqrmhlrdgf ladqldpiyv aynm
//
LOCUS       XP_076546057            2194 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546057
VERSION     XP_076546057.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689942.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2194
                     /product="disco-interacting protein 2 isoform X1"
                     /calculated_mol_wt=239082
     Region          <565..>582
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          949..1523
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1108,1111..1116,1118..1119)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1111,1231..1232,1290..1295,1400,1403,1414,1503)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1111,1152..1153,1203,1205..1206,1209,1231..1232,
                     1250..1255,1400,1403,1411..1414,1484)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1152,1205..1206,1209,1231,1411..1413,1471,1484)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1606..2190
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1755,1758..1763,1765..1766)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1758,1884..1885,1939..1944,2035,2077,2080,2091,2170)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1758,1799..1800,1850,1852..1853,1856,1884..1885,
                     1902..1907,2035,2077,2080,2088..2091,2151)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1799,1852..1853,1856,1884,2088..2090,2138,2151)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2194
                     /gene="DIP2"
                     /coded_by="XM_076689942.1:2379..8963"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsggrlvpgg iasppgsggs tgntgnsnsa aarrgnrrlt rnesryhsev rqeavqqala
      721 amqgrpkpsl pmpskrtsvm arspdrerrd sgesssdeds vvteespgag gptgtglsdt
      781 sstgsardtp ppprpparrp pgaditdiae yaphaycniq ppdvthtsnt psaqqstrrp
      841 gadrvnryhv vedqnntgtt grwkvsakiq qllntlkrpk rrplpefyed ddieleiaan
      901 pkdpnapkpe ggsmtsavge plsvaaglpr sleaaiqryg sasykapvat vldpngklcv
      961 tltygkllsr shkiaytlln kalsrggdcc lkpgdrialv ypnndpisfm cafygclqag
     1021 ivpvpievpl trrdagsqqi gfllgscgiq valtseaclk glpktaagev vafkgwpklh
     1081 wfvtehlgkt pkdwlppprl tddtpayiey ttdkdgsvmg vtvtrsamla hcraltqacg
     1141 ytegenavcv ldfkrevglw hstltsilng mhvifipyal mkvnpaswmq mitkhrasva
     1201 vvksrdlhwg llatkdhkdi slsslrlllv adganpwsls scdqflsvfq skglrpdavc
     1261 pcasssealt vsvrrpgrag vnatgrgvls msglsygvvr vdqensltsl tlqdcgqvmp
     1321 gsivvvikme gqpficktde vgeicvhssa tgnqywglqg ltnntfkvsp lqadgtplgd
     1381 veytrsgllg flgpgglvfv cgsrdglmtv tgrkhnaddi iatvlavepm kfiyrgriav
     1441 fsvrvlrder icvvaeqrpd cseeesfqwm srvlqavdsi havgiyclal vppnhlpktp
     1501 lggihlsetk rrflegtlhp anvllcphtc vtnlpkprev hsagdsvadv gpasvmvgni
     1561 vqgnrlasaq grdmgvlded sdnakkyqfi seilrwravs tsdhviftsl nakgavatsl
     1621 scsqlhkkae rignllldrg rintgdhval ifppgtdlic afygclyvga vpvtirpphp
     1681 qnlqttlptv rmivdvsksv lvltnqnilk llktkeannv vdikswptil dmddmpkkkl
     1741 pvmyraptae mlayldfsvs ttgmlagikm shaavtslcr amklacelyp srhialcldp
     1801 ysglgfalwc lssiysghhs ilippsevea npalwlsavs qsrvrdtfcs ygvmelctkg
     1861 lgssvhalka rgvslacvrt cvvvaeerpr ialttsfskl fsalglspra vstsfgcrvn
     1921 taiclqgass pepstvyvdl ralrndrvsl vergsphslc lmesgkllpg vkviianpet
     1981 kgqcgdshlg eiwvqsahna sgyftiygde sdyadhfnar lvtgntnevy artgylgflr
     2041 rtesvqqsvi sdvpgdtsaa eadlvpgdae lhdavfvvga ldeaillrgm ryhpidiens
     2101 vmrchkkiae cavftwtnll vvvveldgse sealdlvalv tsavleehhl vvgvvvvvdp
     2161 gvvpinsrge kqrmhlrdgf ladqldpiyv aynm
//
LOCUS       XP_076546058            2192 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546058
VERSION     XP_076546058.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689943.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2192
                     /product="disco-interacting protein 2 isoform X2"
                     /calculated_mol_wt=238791
     Region          <565..>582
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          949..1523
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1108,1111..1116,1118..1119)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1111,1231..1232,1290..1295,1400,1403,1414,1503)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1111,1152..1153,1203,1205..1206,1209,1231..1232,
                     1250..1255,1400,1403,1411..1414,1484)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1152,1205..1206,1209,1231,1411..1413,1471,1484)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1604..2188
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1753,1756..1761,1763..1764)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1756,1882..1883,1937..1942,2033,2075,2078,2089,2168)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1756,1797..1798,1848,1850..1851,1854,1882..1883,
                     1900..1905,2033,2075,2078,2086..2089,2149)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1797,1850..1851,1854,1882,2086..2088,2136,2149)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2192
                     /gene="DIP2"
                     /coded_by="XM_076689943.1:3576..10154"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsggrlvpgg iasppgsggs tgntgnsnsa aarrgnrrlt rnesryhsev rqeavqqala
      721 amqgrpkpsl pmpskrtsvm arspdrerrd sgesssdeds vvteespgag gptgtglsdt
      781 sstgsardtp ppprpparrp pgaditdiae yaphaycniq ppdvthtsnt psaqqstrrp
      841 gadrvnryhv vedqnntgtt grwkvsakiq qllntlkrpk rrplpefyed ddieleiaan
      901 pkdpnapkpe ggsmtsavge plsvaaglpr sleaaiqryg sasykapvat vldpngklcv
      961 tltygkllsr shkiaytlln kalsrggdcc lkpgdrialv ypnndpisfm cafygclqag
     1021 ivpvpievpl trrdagsqqi gfllgscgiq valtseaclk glpktaagev vafkgwpklh
     1081 wfvtehlgkt pkdwlppprl tddtpayiey ttdkdgsvmg vtvtrsamla hcraltqacg
     1141 ytegenavcv ldfkrevglw hstltsilng mhvifipyal mkvnpaswmq mitkhrasva
     1201 vvksrdlhwg llatkdhkdi slsslrlllv adganpwsls scdqflsvfq skglrpdavc
     1261 pcasssealt vsvrrpgrag vnatgrgvls msglsygvvr vdqensltsl tlqdcgqvmp
     1321 gsivvvikme gqpficktde vgeicvhssa tgnqywglqg ltnntfkvsp lqadgtplgd
     1381 veytrsgllg flgpgglvfv cgsrdglmtv tgrkhnaddi iatvlavepm kfiyrgriav
     1441 fsvrvlrder icvvaeqrpd cseeesfqwm srvlqavdsi havgiyclal vppnhlpktp
     1501 lggihlsetk rrflegtlhp anvllcphtc vtnlpkprev hsagdsvadv gpasvmvgni
     1561 vqgnrlasaq grdmgvlded sdnakkfise ilrwravsts dhviftslna kgavatslsc
     1621 sqlhkkaeri gnllldrgri ntgdhvalif ppgtdlicaf ygclyvgavp vtirpphpqn
     1681 lqttlptvrm ivdvsksvlv ltnqnilkll ktkeannvvd ikswptildm ddmpkkklpv
     1741 myraptaeml ayldfsvstt gmlagikmsh aavtslcram klacelypsr hialcldpys
     1801 glgfalwcls siysghhsil ippseveanp alwlsavsqs rvrdtfcsyg vmelctkglg
     1861 ssvhalkarg vslacvrtcv vvaeerpria lttsfsklfs alglspravs tsfgcrvnta
     1921 iclqgasspe pstvyvdlra lrndrvslve rgsphslclm esgkllpgvk viianpetkg
     1981 qcgdshlgei wvqsahnasg yftiygdesd yadhfnarlv tgntnevyar tgylgflrrt
     2041 esvqqsvisd vpgdtsaaea dlvpgdaelh davfvvgald eaillrgmry hpidiensvm
     2101 rchkkiaeca vftwtnllvv vveldgsese aldlvalvts avleehhlvv gvvvvvdpgv
     2161 vpinsrgekq rmhlrdgfla dqldpiyvay nm
//
LOCUS       XP_076546059            2188 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546059
VERSION     XP_076546059.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689944.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2188
                     /product="disco-interacting protein 2 isoform X3"
                     /calculated_mol_wt=238581
     Region          <565..>582
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          949..1523
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1108,1111..1116,1118..1119)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1111,1231..1232,1290..1295,1400,1403,1414,1503)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1111,1152..1153,1203,1205..1206,1209,1231..1232,
                     1250..1255,1400,1403,1411..1414,1484)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1152,1205..1206,1209,1231,1411..1413,1471,1484)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1600..2184
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1749,1752..1757,1759..1760)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1752,1878..1879,1933..1938,2029,2071,2074,2085,2164)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1752,1793..1794,1844,1846..1847,1850,1878..1879,
                     1896..1901,2029,2071,2074,2082..2085,2145)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1793,1846..1847,1850,1878,2082..2084,2132,2145)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2188
                     /gene="DIP2"
                     /coded_by="XM_076689944.1:3577..10143"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsggrlvpgg iasppgsggs tgntgnsnsa aarrgnrrlt rnesryhsev rqeavqqala
      721 amqgrpkpsl pmpskrtsvm arspdrerrd sgesssdeds vvteespgag gptgtglsdt
      781 sstgsardtp ppprpparrp pgaditdiae yaphaycniq ppdvthtsnt psaqqstrrp
      841 gadrvnryhv vedqnntgtt grwkvsakiq qllntlkrpk rrplpefyed ddieleiaan
      901 pkdpnapkpe ggsmtsavge plsvaaglpr sleaaiqryg sasykapvat vldpngklcv
      961 tltygkllsr shkiaytlln kalsrggdcc lkpgdrialv ypnndpisfm cafygclqag
     1021 ivpvpievpl trrdagsqqi gfllgscgiq valtseaclk glpktaagev vafkgwpklh
     1081 wfvtehlgkt pkdwlppprl tddtpayiey ttdkdgsvmg vtvtrsamla hcraltqacg
     1141 ytegenavcv ldfkrevglw hstltsilng mhvifipyal mkvnpaswmq mitkhrasva
     1201 vvksrdlhwg llatkdhkdi slsslrlllv adganpwsls scdqflsvfq skglrpdavc
     1261 pcasssealt vsvrrpgrag vnatgrgvls msglsygvvr vdqensltsl tlqdcgqvmp
     1321 gsivvvikme gqpficktde vgeicvhssa tgnqywglqg ltnntfkvsp lqadgtplgd
     1381 veytrsgllg flgpgglvfv cgsrdglmtv tgrkhnaddi iatvlavepm kfiyrgriav
     1441 fsvrvlrder icvvaeqrpd cseeesfqwm srvlqavdsi havgiyclal vppnhlpktp
     1501 lggihlsetk rrflegtlhp anvllcphtc vtnlpkprev hsdvgpasvm vgnivqgnrl
     1561 asaqgrdmgv ldedsdnakk yqfiseilrw ravstsdhvi ftslnakgav atslscsqlh
     1621 kkaerignll ldrgrintgd hvalifppgt dlicafygcl yvgavpvtir pphpqnlqtt
     1681 lptvrmivdv sksvlvltnq nilkllktke annvvdiksw ptildmddmp kkklpvmyra
     1741 ptaemlayld fsvsttgmla gikmshaavt slcramklac elypsrhial cldpysglgf
     1801 alwclssiys ghhsilipps eveanpalwl savsqsrvrd tfcsygvmel ctkglgssvh
     1861 alkargvsla cvrtcvvvae erprialtts fsklfsalgl spravstsfg crvntaiclq
     1921 gasspepstv yvdlralrnd rvslvergsp hslclmesgk llpgvkviia npetkgqcgd
     1981 shlgeiwvqs ahnasgyfti ygdesdyadh fnarlvtgnt nevyartgyl gflrrtesvq
     2041 qsvisdvpgd tsaaeadlvp gdaelhdavf vvgaldeail lrgmryhpid iensvmrchk
     2101 kiaecavftw tnllvvvvel dgsesealdl valvtsavle ehhlvvgvvv vvdpgvvpin
     2161 srgekqrmhl rdgfladqld piyvaynm
//
LOCUS       XP_076546060            2186 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546060
VERSION     XP_076546060.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689945.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2186
                     /product="disco-interacting protein 2 isoform X4"
                     /calculated_mol_wt=238290
     Region          <565..>582
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          949..1523
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1108,1111..1116,1118..1119)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1111,1231..1232,1290..1295,1400,1403,1414,1503)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1111,1152..1153,1203,1205..1206,1209,1231..1232,
                     1250..1255,1400,1403,1411..1414,1484)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1152,1205..1206,1209,1231,1411..1413,1471,1484)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1598..2182
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1747,1750..1755,1757..1758)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1750,1876..1877,1931..1936,2027,2069,2072,2083,2162)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1750,1791..1792,1842,1844..1845,1848,1876..1877,
                     1894..1899,2027,2069,2072,2080..2083,2143)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1791,1844..1845,1848,1876,2080..2082,2130,2143)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2186
                     /gene="DIP2"
                     /coded_by="XM_076689945.1:3578..10138"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsggrlvpgg iasppgsggs tgntgnsnsa aarrgnrrlt rnesryhsev rqeavqqala
      721 amqgrpkpsl pmpskrtsvm arspdrerrd sgesssdeds vvteespgag gptgtglsdt
      781 sstgsardtp ppprpparrp pgaditdiae yaphaycniq ppdvthtsnt psaqqstrrp
      841 gadrvnryhv vedqnntgtt grwkvsakiq qllntlkrpk rrplpefyed ddieleiaan
      901 pkdpnapkpe ggsmtsavge plsvaaglpr sleaaiqryg sasykapvat vldpngklcv
      961 tltygkllsr shkiaytlln kalsrggdcc lkpgdrialv ypnndpisfm cafygclqag
     1021 ivpvpievpl trrdagsqqi gfllgscgiq valtseaclk glpktaagev vafkgwpklh
     1081 wfvtehlgkt pkdwlppprl tddtpayiey ttdkdgsvmg vtvtrsamla hcraltqacg
     1141 ytegenavcv ldfkrevglw hstltsilng mhvifipyal mkvnpaswmq mitkhrasva
     1201 vvksrdlhwg llatkdhkdi slsslrlllv adganpwsls scdqflsvfq skglrpdavc
     1261 pcasssealt vsvrrpgrag vnatgrgvls msglsygvvr vdqensltsl tlqdcgqvmp
     1321 gsivvvikme gqpficktde vgeicvhssa tgnqywglqg ltnntfkvsp lqadgtplgd
     1381 veytrsgllg flgpgglvfv cgsrdglmtv tgrkhnaddi iatvlavepm kfiyrgriav
     1441 fsvrvlrder icvvaeqrpd cseeesfqwm srvlqavdsi havgiyclal vppnhlpktp
     1501 lggihlsetk rrflegtlhp anvllcphtc vtnlpkprev hsdvgpasvm vgnivqgnrl
     1561 asaqgrdmgv ldedsdnakk fiseilrwra vstsdhvift slnakgavat slscsqlhkk
     1621 aerignllld rgrintgdhv alifppgtdl icafygclyv gavpvtirpp hpqnlqttlp
     1681 tvrmivdvsk svlvltnqni lkllktkean nvvdikswpt ildmddmpkk klpvmyrapt
     1741 aemlayldfs vsttgmlagi kmshaavtsl cramklacel ypsrhialcl dpysglgfal
     1801 wclssiysgh hsilippsev eanpalwlsa vsqsrvrdtf csygvmelct kglgssvhal
     1861 kargvslacv rtcvvvaeer prialttsfs klfsalglsp ravstsfgcr vntaiclqga
     1921 sspepstvyv dlralrndrv slvergsphs lclmesgkll pgvkviianp etkgqcgdsh
     1981 lgeiwvqsah nasgyftiyg desdyadhfn arlvtgntne vyartgylgf lrrtesvqqs
     2041 visdvpgdts aaeadlvpgd aelhdavfvv galdeaillr gmryhpidie nsvmrchkki
     2101 aecavftwtn llvvvveldg sesealdlva lvtsavleeh hlvvgvvvvv dpgvvpinsr
     2161 gekqrmhlrd gfladqldpi yvaynm
//
LOCUS       XP_076546061            2148 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546061
VERSION     XP_076546061.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689946.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2148
                     /product="disco-interacting protein 2 isoform X5"
                     /calculated_mol_wt=234491
     Region          <565..694
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          903..1477
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1062,1065..1070,1072..1073)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1065,1185..1186,1244..1249,1354,1357,1368,1457)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1065,1106..1107,1157,1159..1160,1163,1185..1186,
                     1204..1209,1354,1357,1365..1368,1438)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1106,1159..1160,1163,1185,1365..1367,1425,1438)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1560..2144
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1709,1712..1717,1719..1720)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1712,1838..1839,1893..1898,1989,2031,2034,2045,2124)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1712,1753..1754,1804,1806..1807,1810,1838..1839,
                     1856..1861,1989,2031,2034,2042..2045,2105)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1753,1806..1807,1810,1838,2042..2044,2092,2105)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2148
                     /gene="DIP2"
                     /coded_by="XM_076689946.1:3575..10021"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsevrqeavq qalaamqgrp kpslpmpskr tsvmarspdr errdsgesss dedsvvtees
      721 pgaggptgtg lsdtsstgsa rdtpppprpp arrppgadit diaeyaphay cniqppdvth
      781 tsntpsaqqs trrpgadrvn ryhvvedqnn tgttgrwkvs akiqqllntl krpkrrplpe
      841 fyedddiele iaanpkdpna pkpeggsmts avgeplsvaa glprsleaai qrygsasyka
      901 pvatvldpng klcvtltygk llsrshkiay tllnkalsrg gdcclkpgdr ialvypnndp
      961 isfmcafygc lqagivpvpi evpltrrdag sqqigfllgs cgiqvaltse aclkglpkta
     1021 agevvafkgw pklhwfvteh lgktpkdwlp pprltddtpa yieyttdkdg svmgvtvtrs
     1081 amlahcralt qacgytegen avcvldfkre vglwhstlts ilngmhvifi pyalmkvnpa
     1141 swmqmitkhr asvavvksrd lhwgllatkd hkdislsslr lllvadganp wslsscdqfl
     1201 svfqskglrp davcpcasss ealtvsvrrp gragvnatgr gvlsmsglsy gvvrvdqens
     1261 ltsltlqdcg qvmpgsivvv ikmegqpfic ktdevgeicv hssatgnqyw glqgltnntf
     1321 kvsplqadgt plgdveytrs gllgflgpgg lvfvcgsrdg lmtvtgrkhn addiiatvla
     1381 vepmkfiyrg riavfsvrvl rdericvvae qrpdcseees fqwmsrvlqa vdsihavgiy
     1441 clalvppnhl pktplggihl setkrrfleg tlhpanvllc phtcvtnlpk prevhsagds
     1501 vadvgpasvm vgnivqgnrl asaqgrdmgv ldedsdnakk yqfiseilrw ravstsdhvi
     1561 ftslnakgav atslscsqlh kkaerignll ldrgrintgd hvalifppgt dlicafygcl
     1621 yvgavpvtir pphpqnlqtt lptvrmivdv sksvlvltnq nilkllktke annvvdiksw
     1681 ptildmddmp kkklpvmyra ptaemlayld fsvsttgmla gikmshaavt slcramklac
     1741 elypsrhial cldpysglgf alwclssiys ghhsilipps eveanpalwl savsqsrvrd
     1801 tfcsygvmel ctkglgssvh alkargvsla cvrtcvvvae erprialtts fsklfsalgl
     1861 spravstsfg crvntaiclq gasspepstv yvdlralrnd rvslvergsp hslclmesgk
     1921 llpgvkviia npetkgqcgd shlgeiwvqs ahnasgyfti ygdesdyadh fnarlvtgnt
     1981 nevyartgyl gflrrtesvq qsvisdvpgd tsaaeadlvp gdaelhdavf vvgaldeail
     2041 lrgmryhpid iensvmrchk kiaecavftw tnllvvvvel dgsesealdl valvtsavle
     2101 ehhlvvgvvv vvdpgvvpin srgekqrmhl rdgfladqld piyvaynm
//
LOCUS       XP_076546062            2119 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076546062
VERSION     XP_076546062.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689947.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2119
                     /product="disco-interacting protein 2 isoform X6"
                     /calculated_mol_wt=232098
     Region          <565..665
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          874..1448
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1033,1036..1041,1043..1044)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1036,1156..1157,1215..1220,1325,1328,1339,1428)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1036,1077..1078,1128,1130..1131,1134,1156..1157,
                     1175..1180,1325,1328,1336..1339,1409)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1077,1130..1131,1134,1156,1336..1338,1396,1409)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1531..2115
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1680,1683..1688,1690..1691)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1683,1809..1810,1864..1869,1960,2002,2005,2016,2095)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1683,1724..1725,1775,1777..1778,1781,1809..1810,
                     1827..1832,1960,2002,2005,2013..2016,2076)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1724,1777..1778,1781,1809,2013..2015,2063,2076)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2119
                     /gene="DIP2"
                     /coded_by="XM_076689947.1:3573..9932"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlggr lvpggiaspp
      601 gsggstgntg nsnsaaarrg nrrltrnesr yhsevrqeav qqalaamqgr pkpslpmpsk
      661 rtsvmarspd rerrdsgess sdedsvvtee spgaggptgt glsdtsstgs ardtpppprp
      721 parrppgadi tdiaeyapha ycniqppdvt htsntpsaqq strrpgadrv nryhvvedqn
      781 ntgttgrwkv sakiqqllnt lkrpkrrplp efyedddiel eiaanpkdpn apkpeggsmt
      841 savgeplsva aglprsleaa iqrygsasyk apvatvldpn gklcvtltyg kllsrshkia
      901 ytllnkalsr ggdcclkpgd rialvypnnd pisfmcafyg clqagivpvp ievpltrrda
      961 gsqqigfllg scgiqvalts eaclkglpkt aagevvafkg wpklhwfvte hlgktpkdwl
     1021 ppprltddtp ayieyttdkd gsvmgvtvtr samlahcral tqacgytege navcvldfkr
     1081 evglwhstlt silngmhvif ipyalmkvnp aswmqmitkh rasvavvksr dlhwgllatk
     1141 dhkdislssl rlllvadgan pwslsscdqf lsvfqskglr pdavcpcass sealtvsvrr
     1201 pgragvnatg rgvlsmsgls ygvvrvdqen sltsltlqdc gqvmpgsivv vikmegqpfi
     1261 cktdevgeic vhssatgnqy wglqgltnnt fkvsplqadg tplgdveytr sgllgflgpg
     1321 glvfvcgsrd glmtvtgrkh naddiiatvl avepmkfiyr griavfsvrv lrdericvva
     1381 eqrpdcseee sfqwmsrvlq avdsihavgi yclalvppnh lpktplggih lsetkrrfle
     1441 gtlhpanvll cphtcvtnlp kprevhsagd svadvgpasv mvgnivqgnr lasaqgrdmg
     1501 vldedsdnak kyqfiseilr wravstsdhv iftslnakga vatslscsql hkkaerignl
     1561 lldrgrintg dhvalifppg tdlicafygc lyvgavpvti rpphpqnlqt tlptvrmivd
     1621 vsksvlvltn qnilkllktk eannvvdiks wptildmddm pkkklpvmyr aptaemlayl
     1681 dfsvsttgml agikmshaav tslcramkla celypsrhia lcldpysglg falwclssiy
     1741 sghhsilipp seveanpalw lsavsqsrvr dtfcsygvme lctkglgssv halkargvsl
     1801 acvrtcvvva eerprialtt sfsklfsalg lspravstsf gcrvntaicl qgasspepst
     1861 vyvdlralrn drvslvergs phslclmesg kllpgvkvii anpetkgqcg dshlgeiwvq
     1921 sahnasgyft iygdesdyad hfnarlvtgn tnevyartgy lgflrrtesv qqsvisdvpg
     1981 dtsaaeadlv pgdaelhdav fvvgaldeai llrgmryhpi diensvmrch kkiaecavft
     2041 wtnllvvvve ldgseseald lvalvtsavl eehhlvvgvv vvvdpgvvpi nsrgekqrmh
     2101 lrdgfladql dpiyvaynm
//
LOCUS       XP_076546063            2073 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546063
VERSION     XP_076546063.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689948.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2073
                     /product="disco-interacting protein 2 isoform X7"
                     /calculated_mol_wt=227507
     Region          <565..619
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          828..1402
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(987,990..995,997..998)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(990,1110..1111,1169..1174,1279,1282,1293,1382)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(990,1031..1032,1082,1084..1085,1088,1110..1111,
                     1129..1134,1279,1282,1290..1293,1363)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1031,1084..1085,1088,1110,1290..1292,1350,1363)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1485..2069
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1634,1637..1642,1644..1645)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1637,1763..1764,1818..1823,1914,1956,1959,1970,2049)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1637,1678..1679,1729,1731..1732,1735,1763..1764,
                     1781..1786,1914,1956,1959,1967..1970,2030)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1678,1731..1732,1735,1763,1967..1969,2017,2030)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2073
                     /gene="DIP2"
                     /coded_by="XM_076689948.1:3573..9794"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlevr qeavqqalaa
      601 mqgrpkpslp mpskrtsvma rspdrerrds gesssdedsv vteespgagg ptgtglsdts
      661 stgsardtpp pprpparrpp gaditdiaey aphaycniqp pdvthtsntp saqqstrrpg
      721 adrvnryhvv edqnntgttg rwkvsakiqq llntlkrpkr rplpefyedd dieleiaanp
      781 kdpnapkpeg gsmtsavgep lsvaaglprs leaaiqrygs asykapvatv ldpngklcvt
      841 ltygkllsrs hkiaytllnk alsrggdccl kpgdrialvy pnndpisfmc afygclqagi
      901 vpvpievplt rrdagsqqig fllgscgiqv altseaclkg lpktaagevv afkgwpklhw
      961 fvtehlgktp kdwlppprlt ddtpayieyt tdkdgsvmgv tvtrsamlah craltqacgy
     1021 tegenavcvl dfkrevglwh stltsilngm hvifipyalm kvnpaswmqm itkhrasvav
     1081 vksrdlhwgl latkdhkdis lsslrlllva dganpwslss cdqflsvfqs kglrpdavcp
     1141 casssealtv svrrpgragv natgrgvlsm sglsygvvrv dqensltslt lqdcgqvmpg
     1201 sivvvikmeg qpficktdev geicvhssat gnqywglqgl tnntfkvspl qadgtplgdv
     1261 eytrsgllgf lgpgglvfvc gsrdglmtvt grkhnaddii atvlavepmk fiyrgriavf
     1321 svrvlrderi cvvaeqrpdc seeesfqwms rvlqavdsih avgiyclalv ppnhlpktpl
     1381 ggihlsetkr rflegtlhpa nvllcphtcv tnlpkprevh sagdsvadvg pasvmvgniv
     1441 qgnrlasaqg rdmgvldeds dnakkyqfis eilrwravst sdhviftsln akgavatsls
     1501 csqlhkkaer ignllldrgr intgdhvali fppgtdlica fygclyvgav pvtirpphpq
     1561 nlqttlptvr mivdvsksvl vltnqnilkl lktkeannvv dikswptild mddmpkkklp
     1621 vmyraptaem layldfsvst tgmlagikms haavtslcra mklacelyps rhialcldpy
     1681 sglgfalwcl ssiysghhsi lippsevean palwlsavsq srvrdtfcsy gvmelctkgl
     1741 gssvhalkar gvslacvrtc vvvaeerpri alttsfsklf salglsprav stsfgcrvnt
     1801 aiclqgassp epstvyvdlr alrndrvslv ergsphslcl mesgkllpgv kviianpetk
     1861 gqcgdshlge iwvqsahnas gyftiygdes dyadhfnarl vtgntnevya rtgylgflrr
     1921 tesvqqsvis dvpgdtsaae adlvpgdael hdavfvvgal deaillrgmr yhpidiensv
     1981 mrchkkiaec avftwtnllv vvveldgses ealdlvalvt savleehhlv vgvvvvvdpg
     2041 vvpinsrgek qrmhlrdgfl adqldpiyva ynm
//
LOCUS       XP_076546064            1659 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_076546064
VERSION     XP_076546064.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689949.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1659
                     /product="disco-interacting protein 2 isoform X8"
                     /calculated_mol_wt=178059
     Region          11..>47
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          414..988
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(573,576..581,583..584)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(576,696..697,755..760,865,868,879,968)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(576,617..618,668,670..671,674,696..697,715..720,865,
                     868,876..879,949)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(617,670..671,674,696,876..878,936,949)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1071..1655
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1220,1223..1228,1230..1231)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1223,1349..1350,1404..1409,1500,1542,1545,1556,1635)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1223,1264..1265,1315,1317..1318,1321,1349..1350,
                     1367..1372,1500,1542,1545,1553..1556,1616)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1264,1317..1318,1321,1349,1553..1555,1603,1616)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1659
                     /gene="DIP2"
                     /coded_by="XM_076689949.1:55..5034"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 maefnidigk lpedvrekla eldlelsegd itqkgyekkr trllqqyask qlgaendggg
       61 ggggggvcvl gitgvertgy gsgggggggg ggvvggigvg vgvggrpqpr arrtqrrvth
      121 nekryhsggr lvpggiaspp gsggstgntg nsnsaaarrg nrrltrnesr yhsevrqeav
      181 qqalaamqgr pkpslpmpsk rtsvmarspd rerrdsgess sdedsvvtee spgaggptgt
      241 glsdtsstgs ardtpppprp parrppgadi tdiaeyapha ycniqppdvt htsntpsaqq
      301 strrpgadrv nryhvvedqn ntgttgrwkv sakiqqllnt lkrpkrrplp efyedddiel
      361 eiaanpkdpn apkpeggsmt savgeplsva aglprsleaa iqrygsasyk apvatvldpn
      421 gklcvtltyg kllsrshkia ytllnkalsr ggdcclkpgd rialvypnnd pisfmcafyg
      481 clqagivpvp ievpltrrda gsqqigfllg scgiqvalts eaclkglpkt aagevvafkg
      541 wpklhwfvte hlgktpkdwl ppprltddtp ayieyttdkd gsvmgvtvtr samlahcral
      601 tqacgytege navcvldfkr evglwhstlt silngmhvif ipyalmkvnp aswmqmitkh
      661 rasvavvksr dlhwgllatk dhkdislssl rlllvadgan pwslsscdqf lsvfqskglr
      721 pdavcpcass sealtvsvrr pgragvnatg rgvlsmsgls ygvvrvdqen sltsltlqdc
      781 gqvmpgsivv vikmegqpfi cktdevgeic vhssatgnqy wglqgltnnt fkvsplqadg
      841 tplgdveytr sgllgflgpg glvfvcgsrd glmtvtgrkh naddiiatvl avepmkfiyr
      901 griavfsvrv lrdericvva eqrpdcseee sfqwmsrvlq avdsihavgi yclalvppnh
      961 lpktplggih lsetkrrfle gtlhpanvll cphtcvtnlp kprevhsagd svadvgpasv
     1021 mvgnivqgnr lasaqgrdmg vldedsdnak kyqfiseilr wravstsdhv iftslnakga
     1081 vatslscsql hkkaerignl lldrgrintg dhvalifppg tdlicafygc lyvgavpvti
     1141 rpphpqnlqt tlptvrmivd vsksvlvltn qnilkllktk eannvvdiks wptildmddm
     1201 pkkklpvmyr aptaemlayl dfsvsttgml agikmshaav tslcramkla celypsrhia
     1261 lcldpysglg falwclssiy sghhsilipp seveanpalw lsavsqsrvr dtfcsygvme
     1321 lctkglgssv halkargvsl acvrtcvvva eerprialtt sfsklfsalg lspravstsf
     1381 gcrvntaicl qgasspepst vyvdlralrn drvslvergs phslclmesg kllpgvkvii
     1441 anpetkgqcg dshlgeiwvq sahnasgyft iygdesdyad hfnarlvtgn tnevyartgy
     1501 lgflrrtesv qqsvisdvpg dtsaaeadlv pgdaelhdav fvvgaldeai llrgmryhpi
     1561 diensvmrch kkiaecavft wtnllvvvve ldgseseald lvalvtsavl eehhlvvgvv
     1621 vvvdpgvvpi nsrgekqrmh lrdgfladql dpiyvaynm
//
LOCUS       XP_076546065            1584 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_076546065
VERSION     XP_076546065.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689950.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1584
                     /product="disco-interacting protein 2 isoform X9"
                     /calculated_mol_wt=171076
     Region          11..130
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          339..913
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(498,501..506,508..509)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(501,621..622,680..685,790,793,804,893)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(501,542..543,593,595..596,599,621..622,640..645,790,
                     793,801..804,874)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(542,595..596,599,621,801..803,861,874)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          996..1580
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1145,1148..1153,1155..1156)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1148,1274..1275,1329..1334,1425,1467,1470,1481,1560)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1148,1189..1190,1240,1242..1243,1246,1274..1275,
                     1292..1297,1425,1467,1470,1478..1481,1541)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1189,1242..1243,1246,1274,1478..1480,1528,1541)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1584
                     /gene="DIP2"
                     /coded_by="XM_076689950.1:55..4809"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 maefnidigk lpedvrekla eldlelsegd itqkgyekkr trllqqyask qlggrlvpgg
       61 iasppgsggs tgntgnsnsa aarrgnrrlt rnesryhsev rqeavqqala amqgrpkpsl
      121 pmpskrtsvm arspdrerrd sgesssdeds vvteespgag gptgtglsdt sstgsardtp
      181 ppprpparrp pgaditdiae yaphaycniq ppdvthtsnt psaqqstrrp gadrvnryhv
      241 vedqnntgtt grwkvsakiq qllntlkrpk rrplpefyed ddieleiaan pkdpnapkpe
      301 ggsmtsavge plsvaaglpr sleaaiqryg sasykapvat vldpngklcv tltygkllsr
      361 shkiaytlln kalsrggdcc lkpgdrialv ypnndpisfm cafygclqag ivpvpievpl
      421 trrdagsqqi gfllgscgiq valtseaclk glpktaagev vafkgwpklh wfvtehlgkt
      481 pkdwlppprl tddtpayiey ttdkdgsvmg vtvtrsamla hcraltqacg ytegenavcv
      541 ldfkrevglw hstltsilng mhvifipyal mkvnpaswmq mitkhrasva vvksrdlhwg
      601 llatkdhkdi slsslrlllv adganpwsls scdqflsvfq skglrpdavc pcasssealt
      661 vsvrrpgrag vnatgrgvls msglsygvvr vdqensltsl tlqdcgqvmp gsivvvikme
      721 gqpficktde vgeicvhssa tgnqywglqg ltnntfkvsp lqadgtplgd veytrsgllg
      781 flgpgglvfv cgsrdglmtv tgrkhnaddi iatvlavepm kfiyrgriav fsvrvlrder
      841 icvvaeqrpd cseeesfqwm srvlqavdsi havgiyclal vppnhlpktp lggihlsetk
      901 rrflegtlhp anvllcphtc vtnlpkprev hsagdsvadv gpasvmvgni vqgnrlasaq
      961 grdmgvlded sdnakkyqfi seilrwravs tsdhviftsl nakgavatsl scsqlhkkae
     1021 rignllldrg rintgdhval ifppgtdlic afygclyvga vpvtirpphp qnlqttlptv
     1081 rmivdvsksv lvltnqnilk llktkeannv vdikswptil dmddmpkkkl pvmyraptae
     1141 mlayldfsvs ttgmlagikm shaavtslcr amklacelyp srhialcldp ysglgfalwc
     1201 lssiysghhs ilippsevea npalwlsavs qsrvrdtfcs ygvmelctkg lgssvhalka
     1261 rgvslacvrt cvvvaeerpr ialttsfskl fsalglspra vstsfgcrvn taiclqgass
     1321 pepstvyvdl ralrndrvsl vergsphslc lmesgkllpg vkviianpet kgqcgdshlg
     1381 eiwvqsahna sgyftiygde sdyadhfnar lvtgntnevy artgylgflr rtesvqqsvi
     1441 sdvpgdtsaa eadlvpgdae lhdavfvvga ldeaillrgm ryhpidiens vmrchkkiae
     1501 cavftwtnll vvvveldgse sealdlvalv tsavleehhl vvgvvvvvdp gvvpinsrge
     1561 kqrmhlrdgf ladqldpiyv aynm
//
LOCUS       XP_076546066            1613 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_076546066
VERSION     XP_076546066.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689951.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1613
                     /product="disco-interacting protein 2 isoform X10"
                     /calculated_mol_wt=173468
     Region          11..159
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          368..942
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(527,530..535,537..538)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(530,650..651,709..714,819,822,833,922)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(530,571..572,622,624..625,628,650..651,669..674,819,
                     822,830..833,903)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(571,624..625,628,650,830..832,890,903)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1025..1609
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1174,1177..1182,1184..1185)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1177,1303..1304,1358..1363,1454,1496,1499,1510,1589)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1177,1218..1219,1269,1271..1272,1275,1303..1304,
                     1321..1326,1454,1496,1499,1507..1510,1570)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1218,1271..1272,1275,1303,1507..1509,1557,1570)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..1613
                     /gene="DIP2"
                     /coded_by="XM_076689951.1:55..4896"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 maefnidigk lpedvrekla eldlelsegd itqkgyekkr trllqqyask qlgaendggg
       61 ggggggvcvl gitgvertgy gsgggggggg ggvvggigvg vgvggrpqpr arrtqrrvth
      121 nekryhsevr qeavqqalaa mqgrpkpslp mpskrtsvma rspdrerrds gesssdedsv
      181 vteespgagg ptgtglsdts stgsardtpp pprpparrpp gaditdiaey aphaycniqp
      241 pdvthtsntp saqqstrrpg adrvnryhvv edqnntgttg rwkvsakiqq llntlkrpkr
      301 rplpefyedd dieleiaanp kdpnapkpeg gsmtsavgep lsvaaglprs leaaiqrygs
      361 asykapvatv ldpngklcvt ltygkllsrs hkiaytllnk alsrggdccl kpgdrialvy
      421 pnndpisfmc afygclqagi vpvpievplt rrdagsqqig fllgscgiqv altseaclkg
      481 lpktaagevv afkgwpklhw fvtehlgktp kdwlppprlt ddtpayieyt tdkdgsvmgv
      541 tvtrsamlah craltqacgy tegenavcvl dfkrevglwh stltsilngm hvifipyalm
      601 kvnpaswmqm itkhrasvav vksrdlhwgl latkdhkdis lsslrlllva dganpwslss
      661 cdqflsvfqs kglrpdavcp casssealtv svrrpgragv natgrgvlsm sglsygvvrv
      721 dqensltslt lqdcgqvmpg sivvvikmeg qpficktdev geicvhssat gnqywglqgl
      781 tnntfkvspl qadgtplgdv eytrsgllgf lgpgglvfvc gsrdglmtvt grkhnaddii
      841 atvlavepmk fiyrgriavf svrvlrderi cvvaeqrpdc seeesfqwms rvlqavdsih
      901 avgiyclalv ppnhlpktpl ggihlsetkr rflegtlhpa nvllcphtcv tnlpkprevh
      961 sagdsvadvg pasvmvgniv qgnrlasaqg rdmgvldeds dnakkyqfis eilrwravst
     1021 sdhviftsln akgavatsls csqlhkkaer ignllldrgr intgdhvali fppgtdlica
     1081 fygclyvgav pvtirpphpq nlqttlptvr mivdvsksvl vltnqnilkl lktkeannvv
     1141 dikswptild mddmpkkklp vmyraptaem layldfsvst tgmlagikms haavtslcra
     1201 mklacelyps rhialcldpy sglgfalwcl ssiysghhsi lippsevean palwlsavsq
     1261 srvrdtfcsy gvmelctkgl gssvhalkar gvslacvrtc vvvaeerpri alttsfsklf
     1321 salglsprav stsfgcrvnt aiclqgassp epstvyvdlr alrndrvslv ergsphslcl
     1381 mesgkllpgv kviianpetk gqcgdshlge iwvqsahnas gyftiygdes dyadhfnarl
     1441 vtgntnevya rtgylgflrr tesvqqsvis dvpgdtsaae adlvpgdael hdavfvvgal
     1501 deaillrgmr yhpidiensv mrchkkiaec avftwtnllv vvveldgses ealdlvalvt
     1561 savleehhlv vgvvvvvdpg vvpinsrgek qrmhlrdgfl adqldpiyva ynm
//
LOCUS       XP_076546067            2146 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_076546067
VERSION     XP_076546067.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689952.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2146
                     /product="disco-interacting protein 2 isoform X11"
                     /calculated_mol_wt=234200
     Region          <565..694
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          903..1477
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1062,1065..1070,1072..1073)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1065,1185..1186,1244..1249,1354,1357,1368,1457)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1065,1106..1107,1157,1159..1160,1163,1185..1186,
                     1204..1209,1354,1357,1365..1368,1438)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1106,1159..1160,1163,1185,1365..1367,1425,1438)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1558..2142
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1707,1710..1715,1717..1718)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1710,1836..1837,1891..1896,1987,2029,2032,2043,2122)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1710,1751..1752,1802,1804..1805,1808,1836..1837,
                     1854..1859,1987,2029,2032,2040..2043,2103)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1751,1804..1805,1808,1836,2040..2042,2090,2103)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2146
                     /gene="DIP2"
                     /coded_by="XM_076689952.1:3576..10016"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsevrqeavq qalaamqgrp kpslpmpskr tsvmarspdr errdsgesss dedsvvtees
      721 pgaggptgtg lsdtsstgsa rdtpppprpp arrppgadit diaeyaphay cniqppdvth
      781 tsntpsaqqs trrpgadrvn ryhvvedqnn tgttgrwkvs akiqqllntl krpkrrplpe
      841 fyedddiele iaanpkdpna pkpeggsmts avgeplsvaa glprsleaai qrygsasyka
      901 pvatvldpng klcvtltygk llsrshkiay tllnkalsrg gdcclkpgdr ialvypnndp
      961 isfmcafygc lqagivpvpi evpltrrdag sqqigfllgs cgiqvaltse aclkglpkta
     1021 agevvafkgw pklhwfvteh lgktpkdwlp pprltddtpa yieyttdkdg svmgvtvtrs
     1081 amlahcralt qacgytegen avcvldfkre vglwhstlts ilngmhvifi pyalmkvnpa
     1141 swmqmitkhr asvavvksrd lhwgllatkd hkdislsslr lllvadganp wslsscdqfl
     1201 svfqskglrp davcpcasss ealtvsvrrp gragvnatgr gvlsmsglsy gvvrvdqens
     1261 ltsltlqdcg qvmpgsivvv ikmegqpfic ktdevgeicv hssatgnqyw glqgltnntf
     1321 kvsplqadgt plgdveytrs gllgflgpgg lvfvcgsrdg lmtvtgrkhn addiiatvla
     1381 vepmkfiyrg riavfsvrvl rdericvvae qrpdcseees fqwmsrvlqa vdsihavgiy
     1441 clalvppnhl pktplggihl setkrrfleg tlhpanvllc phtcvtnlpk prevhsagds
     1501 vadvgpasvm vgnivqgnrl asaqgrdmgv ldedsdnakk fiseilrwra vstsdhvift
     1561 slnakgavat slscsqlhkk aerignllld rgrintgdhv alifppgtdl icafygclyv
     1621 gavpvtirpp hpqnlqttlp tvrmivdvsk svlvltnqni lkllktkean nvvdikswpt
     1681 ildmddmpkk klpvmyrapt aemlayldfs vsttgmlagi kmshaavtsl cramklacel
     1741 ypsrhialcl dpysglgfal wclssiysgh hsilippsev eanpalwlsa vsqsrvrdtf
     1801 csygvmelct kglgssvhal kargvslacv rtcvvvaeer prialttsfs klfsalglsp
     1861 ravstsfgcr vntaiclqga sspepstvyv dlralrndrv slvergsphs lclmesgkll
     1921 pgvkviianp etkgqcgdsh lgeiwvqsah nasgyftiyg desdyadhfn arlvtgntne
     1981 vyartgylgf lrrtesvqqs visdvpgdts aaeadlvpgd aelhdavfvv galdeaillr
     2041 gmryhpidie nsvmrchkki aecavftwtn llvvvveldg sesealdlva lvtsavleeh
     2101 hlvvgvvvvv dpgvvpinsr gekqrmhlrd gfladqldpi yvaynm
//
LOCUS       XP_076546068            2140 aa            linear   INV 03-SEP-2025
DEFINITION  disco-interacting protein 2 isoform X12 [Osmia lignaria lignaria].
ACCESSION   XP_076546068
VERSION     XP_076546068.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689953.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2140
                     /product="disco-interacting protein 2 isoform X12"
                     /calculated_mol_wt=233699
     Region          <565..694
                     /region_name="DMAP_binding"
                     /note="DMAP1-binding Domain; pfam06464"
                     /db_xref="CDD:368923"
     Region          903..1477
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1062,1065..1070,1072..1073)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1065,1185..1186,1244..1249,1354,1357,1368,1457)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1065,1106..1107,1157,1159..1160,1163,1185..1186,
                     1204..1209,1354,1357,1365..1368,1438)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1106,1159..1160,1163,1185,1365..1367,1425,1438)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Region          1552..2136
                     /region_name="Dip2"
                     /note="Disco-interacting protein 2 (Dip2); cd05905"
                     /db_xref="CDD:341231"
     Site            order(1701,1704..1709,1711..1712)
                     /site_type="other"
                     /note="putative acyl-activating enzyme (AAE) consensus
                     motif"
                     /db_xref="CDD:341231"
     Site            order(1704,1830..1831,1885..1890,1981,2023,2026,2037,2116)
                     /site_type="other"
                     /note="putative AMP binding site [chemical binding]"
                     /db_xref="CDD:341231"
     Site            order(1704,1745..1746,1796,1798..1799,1802,1830..1831,
                     1848..1853,1981,2023,2026,2034..2037,2097)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:341231"
     Site            order(1745,1798..1799,1802,1830,2034..2036,2084,2097)
                     /site_type="other"
                     /note="putative CoA binding site [chemical binding]"
                     /db_xref="CDD:341231"
     CDS             1..2140
                     /gene="DIP2"
                     /coded_by="XM_076689953.1:3578..10000"
                     /db_xref="GeneID:117600039"
ORIGIN      
        1 mrplsfenlr rlvgrkkdrn epsfkrsesf krisirksyl drgkrrnklq kslepttvvp
       61 ptidtnsnek pieksvikdq kakklqddtl tresisydew lqgvssssre kldqvhreqq
      121 qnkqnrqhgv aksktsskfq kqndadhvae dlkvlelngs pvlhaksfki sgetvqeksg
      181 sqqdilqiqe psvegppsvs islgriwrda vpvpfpspsg psvhhsldsa lkerkpqptv
      241 artvsapeks vigkdassaf gfslriarla dfraggtrng ffnrrkskps psmsgegyfr
      301 rsnasrrsss rrhgkrasqk tkkssrpprs nspvwfvppe rrrsrrqrrv wreiryfpet
      361 vvpvvekide wssnlsddqf ddaklllsgd fndrpeggsn spvssslgsf satsssssac
      421 rppkisdfne dklfseelrn ngvltrrttw rpitsnyfas nqflsflskg ssnlvhgkdk
      481 trpdslmyyr ylsssdsete desnkfdger rknshvnhrq qqlkrqrsgl rrrplrrksq
      541 lrkassgqpv flvrkcsslr krprditqkg yekkrtrllq qyaskqlgae ndgggggggg
      601 gvcvlgitgv ertgygsggg gggggggvvg gigvgvgvgg rpqprarrtq rrvthnekry
      661 hsevrqeavq qalaamqgrp kpslpmpskr tsvmarspdr errdsgesss dedsvvtees
      721 pgaggptgtg lsdtsstgsa rdtpppprpp arrppgadit diaeyaphay cniqppdvth
      781 tsntpsaqqs trrpgadrvn ryhvvedqnn tgttgrwkvs akiqqllntl krpkrrplpe
      841 fyedddiele iaanpkdpna pkpeggsmts avgeplsvaa glprsleaai qrygsasyka
      901 pvatvldpng klcvtltygk llsrshkiay tllnkalsrg gdcclkpgdr ialvypnndp
      961 isfmcafygc lqagivpvpi evpltrrdag sqqigfllgs cgiqvaltse aclkglpkta
     1021 agevvafkgw pklhwfvteh lgktpkdwlp pprltddtpa yieyttdkdg svmgvtvtrs
     1081 amlahcralt qacgytegen avcvldfkre vglwhstlts ilngmhvifi pyalmkvnpa
     1141 swmqmitkhr asvavvksrd lhwgllatkd hkdislsslr lllvadganp wslsscdqfl
     1201 svfqskglrp davcpcasss ealtvsvrrp gragvnatgr gvlsmsglsy gvvrvdqens
     1261 ltsltlqdcg qvmpgsivvv ikmegqpfic ktdevgeicv hssatgnqyw glqgltnntf
     1321 kvsplqadgt plgdveytrs gllgflgpgg lvfvcgsrdg lmtvtgrkhn addiiatvla
     1381 vepmkfiyrg riavfsvrvl rdericvvae qrpdcseees fqwmsrvlqa vdsihavgiy
     1441 clalvppnhl pktplggihl setkrrfleg tlhpanvllc phtcvtnlpk prevhsdvgp
     1501 asvmvgnivq gnrlasaqgr dmgvldedsd nakkfiseil rwravstsdh viftslnakg
     1561 avatslscsq lhkkaerign llldrgrint gdhvalifpp gtdlicafyg clyvgavpvt
     1621 irpphpqnlq ttlptvrmiv dvsksvlvlt nqnilkllkt keannvvdik swptildmdd
     1681 mpkkklpvmy raptaemlay ldfsvsttgm lagikmshaa vtslcramkl acelypsrhi
     1741 alcldpysgl gfalwclssi ysghhsilip pseveanpal wlsavsqsrv rdtfcsygvm
     1801 elctkglgss vhalkargvs lacvrtcvvv aeerprialt tsfsklfsal glspravsts
     1861 fgcrvntaic lqgasspeps tvyvdlralr ndrvslverg sphslclmes gkllpgvkvi
     1921 ianpetkgqc gdshlgeiwv qsahnasgyf tiygdesdya dhfnarlvtg ntnevyartg
     1981 ylgflrrtes vqqsvisdvp gdtsaaeadl vpgdaelhda vfvvgaldea illrgmryhp
     2041 idiensvmrc hkkiaecavf twtnllvvvv eldgseseal dlvalvtsav leehhlvvgv
     2101 vvvvdpgvvp insrgekqrm hlrdgfladq ldpiyvaynm
//
LOCUS       XP_076546069            1550 aa            linear   INV 03-SEP-2025
DEFINITION  recQ4 helicase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546069
VERSION     XP_076546069.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689954.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1550
                     /product="recQ4 helicase isoform X1"
                     /calculated_mol_wt=174924
     Region          12..58
                     /region_name="RecQL4_SLD2_NTD"
                     /note="N-terminal homeodomain-like domain of metazoan RecQ
                     protein-like 4 (RecQL4), fungal DNA replication regulator
                     SLD2 and similar proteins; cd22289"
                     /db_xref="CDD:412085"
     Region          17..>101
                     /region_name="Drc1-Sld2"
                     /note="DNA replication and checkpoint protein; pfam11719"
                     /db_xref="CDD:371692"
     Region          687..702
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     Region          820..>1207
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     CDS             1..1550
                     /gene="RecQ4"
                     /coded_by="XM_076689954.1:485..5137"
                     /db_xref="GeneID:117600071"
ORIGIN      
        1 meiledptfk tqyqkyknrv klwesrftek hgrkpnkndi keadikikes ykmywklktr
       61 aleetlidit fseevqdnvl dktltqtsve etkednkpls ddnkqnlenv sqkvtentdi
      121 niilstdnkd ienvdprcqn qdaatttslv vknectnmkd vwgdhlsksn eiykkketll
      181 vgrsssfqls qnkftssmfv krnprkslsa skikskseks mniqtnvsee stntethned
      241 gidvnkksts vfnnpvkvty testsgsqsi ntiqqviagh tvsrnlnpaw ldrcakqnnv
      301 eysafdsqrl sgtsdsgies messiyspke ntqmsetskh plisdeedfv cnsdseetwr
      361 nkrirnfkkn fsdqeihcpk rpcldnninv ptmqqlptnm nvhgvhslkv snevkykdfs
      421 ttnviadkdn drsdnttivt nvandndkci densdtettn knstnrrkvr rrikhissdd
      481 sdpdfnennk vegkkkekss ktkrksstrk nkstkeptsk qeknstfktk rtsrkkkyvq
      541 sdddnfleng ttdlettdkg seiygvetlq avprfsinms nqgdlieqct ksvsstatns
      601 tksvalpksk gkltdkekle rkiadgnvne nfvrinlkkk ifvrgkkhfn fskykknqwk
      661 qrkkdlqsse nslvvadyle kkgvsncfkc gevghfsrnc pnsntddlip lgeiddgsef
      721 ltleeaqema sqnaliahan ridrlpekps ysskaeaehe gkeieksdnd dlwepienee
      781 llcghkipde lvtkllppii dtvqplyklk edqscietpa evfetlekfg hksfkpgqek
      841 aimrilsgqs tlvtlstgsg kslcyqlpay lyskysncit lvisplvslm ddqvtgvpff
      901 lsaaclhtnq tekvrnnvmq mvkqgklnil lispeavvag ekstgfgall rqlppiafac
      961 ideahcisqw shnfrpsylm vcrvlkekmg vktvlaltat atktttesiv nhlglhdgma
     1021 gvisdipmpr nlmltvsrde hrdraliell qserfkqcns iivyctrree cvriagllrv
     1081 slqdtnnftk pnsklspiae ayhaglpasr rktvqkafmn gqirivvatv afgmginksd
     1141 iraiihynmp gtfenyvqev gragrdgqia hchmflhpke dgdkwelrrh iyangvdrhs
     1201 irrllqkvfv pcscakiqak drnyrcpghe vaipvdetvi aldisaeiis tllcylelhp
     1261 krfitvlssv yvrarvssyg gpqalkqaaq sspplamaia ldlqkgvshd nssviefpvv
     1321 dvasaigwds gvvkshlknl ewktglfeei rivkipfvil kylafvsvng tskrsnisvr
     1381 ydtlglrvka pgdltdeeld ealetlvdra rfqetaalqq letisatlhk lsmpsvkmcl
     1441 elndevtnks nelkdiirny fegkilvdid svqqtkvend aqivsdvrnl ivsyrdnnft
     1501 graiarifhg iqspnypaya wnkcrfwrah lscnfnalcq iatkeilalr
//
LOCUS       XP_034170952            1527 aa            linear   INV 03-SEP-2025
DEFINITION  recQ4 helicase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034170952
VERSION     XP_034170952.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315061.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034170952.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1527
                     /product="recQ4 helicase isoform X2"
                     /calculated_mol_wt=172247
     Region          12..58
                     /region_name="RecQL4_SLD2_NTD"
                     /note="N-terminal homeodomain-like domain of metazoan RecQ
                     protein-like 4 (RecQL4), fungal DNA replication regulator
                     SLD2 and similar proteins; cd22289"
                     /db_xref="CDD:412085"
     Region          17..>101
                     /region_name="Drc1-Sld2"
                     /note="DNA replication and checkpoint protein; pfam11719"
                     /db_xref="CDD:371692"
     Region          687..702
                     /region_name="zf-CCHC"
                     /note="Zinc knuckle; pfam00098"
                     /db_xref="CDD:395050"
     Region          820..>1207
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     CDS             1..1527
                     /gene="RecQ4"
                     /coded_by="XM_034315061.2:485..5068"
                     /db_xref="GeneID:117600071"
ORIGIN      
        1 meiledptfk tqyqkyknrv klwesrftek hgrkpnkndi keadikikes ykmywklktr
       61 aleetlidit fseevqdnvl dktltqtsve etkednkpls ddnkqnlenv sqkvtentdi
      121 niilstdnkd ienvdprcqn qdaatttslv vknectnmkd vwgdhlsksn eiykkketll
      181 vgrsssfqls qnkftssmfv krnprkslsa skikskseks mniqtnvsee stntethned
      241 gidvnkksts vfnnpvkvty testsgsqsi ntiqqviagh tvsrnlnpaw ldrcakqnnv
      301 eysafdsqrl sgtsdsgies messiyspke ntqmsetskh plisdeedfv cnsdseetwr
      361 nkrirnfkkn fsdqeihcpk rpcldnninv ptmqqlptnm nvhgvhslkv snevkykdfs
      421 ttnviadkdn drsdnttivt nvandndkci densdtettn knstnrrkvr rrikhissdd
      481 sdpdfnennk vegkkkekss ktkrksstrk nkstkeptsk qeknstfktk rtsrkkkyvq
      541 sdddnfleng ttdlettdkg seiygvetlq avprfsinms nqgdlieqct ksvsstatns
      601 tksvalpksk gkltdkekle rkiadgnvne nfvrinlkkk ifvrgkkhfn fskykknqwk
      661 qrkkdlqsse nslvvadyle kkgvsncfkc gevghfsrnc pnsntddlip lgeiddgsef
      721 ltleeaqema sqnaliahan ridrlpekps ysskaeaehe gkeieksdnd dlwepienee
      781 llcghkipde lvtkllppii dtvqplyklk edqscietpa evfetlekfg hksfkpgqek
      841 aimrilsgqs tlvtlstgsg kslcyqlpay lyskysncit lvisplvslm ddqvtgvpff
      901 lsaaclhtnq tekvrnnvmq mvkqgklnil lispeavvag ekstgfgall rqlppiafac
      961 ideahcisqw shnfrpsylm vcrvlkekmg vktvlaltat atktttesiv nhlglhdgma
     1021 gvisdipmpr nlmltvsrde hrdraliell qserfkqcns iivyctrree cvriagllrv
     1081 slqdtnnftk pnsklspiae ayhaglpasr rktvqkafmn gqirivvatv afgmginksd
     1141 iraiihynmp gtfenyvqev gragrdgqia hchmflhpke dgdkwelrrh iyangvdrhs
     1201 irrllqkvfv pcscakiqak drnyrcpghe vaipvdetvi aldisaeiis tllcylelhp
     1261 krfitvlssv yvrarvssyg gpqalkqaaq sspplamaia ldlqkgvshd nssviefpvv
     1321 dvasaigwds gvvkshlknl ewktvngtsk rsnisvrydt lglrvkapgd ltdeeldeal
     1381 etlvdrarfq etaalqqlet isatlhklsm psvkmcleln devtnksnel kdiirnyfeg
     1441 kilvdidsvq qtkvendaqi vsdvrnlivs yrdnnftgra iarifhgiqs pnypayawnk
     1501 crfwrahlsc nfnalcqiat keilalr
//
LOCUS       XP_034170963            1284 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034170963
VERSION     XP_034170963.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315072.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1284
                     /product="anion exchange protein Ae2 isoform X1"
                     /calculated_mol_wt=143755
     Region          345..1283
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1284
                     /gene="Ae2"
                     /coded_by="XM_034315072.2:45..3899"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mpeaaanssg ksflqrasgk vlrwlrrslr tnhqvdghga etgpeldeem ekvfamdtge
       61 kfdvarlgsp sesagsdrdr drgppprygd rdfnqhrkrs yphphmplks irsrsmrrhl
      121 ssegsttaei gqeeengqvq tgngggpegd amdaastahd vdeetaeete nvvgsegeap
      181 iseraasgfn esptvgtprv qfenikeeel ppfqkddvts daapgtthee drkcrrhqkh
      241 ehkrhhhksr kyslqedpqw rkrsgaglpd gagmltrrvs vqpeeastlq eldiddlesh
      301 rsddprgmrr hkaahltvqi grkkeggiph dtfkkmydhs phevfvqlde lhglgeerew
      361 retarwikye edveegadrw grphvaslsf hsllnlrrcl etgvvlldle ekdvpglayr
      421 vveqmvieel ilpedrpvvm ralllrhrhv hdhdrgfrfg gkrnyssyts lqsvgleeee
      481 aareasenhv tqhnlndtkp kilssnlald tnhtvvdmke eltftssned lkrshndyil
      541 kripagaeat vvlvgavdfl dqptiafvrl aegafmpsit evtipvrfmf tllgprnadl
      601 dyheigrsia tlmantsfhk vaykanerre llsainefld dsivlppgdw erqallpfne
      661 lkakseairq rkakaleeks kpiqseaavk kallageeek kppddldplr rtkrpfggli
      721 ndikrrypyy lsdltdglns sclaaaifmy faalctaitf gglmsdkthn iigisetlis
      781 gswtgivmal fstqplviig ttgplllfde slynfclane lefltvrvyv gawmgiiala
      841 iacvegsvlv rlftrfteei ftglisilyi vetfiklyny fvknplldey sfipennlts
      901 yqeplnvaem kvvspetgdt iviplekpdt lipaynaagl linqpntalm ctilclgtfl
      961 gayylrifrn shylgrsarr afgdfgvpis iivfvlidyl akvktekllv peglsptvpg
     1021 rdwivspvgm qkpiplwmam acivpallvy ilvfmetqis eliidkkerk lrkgngyhmd
     1081 ivvvclmnvg cglmgapwcc aasvrslthv savtvmsrth apgdkphive vkeqrvsall
     1141 vailvgvsvl mapllrrvpm svllgvflym gissttgvql fdrvklffmp vkhhgtanyv
     1201 rrvqtykmhi ftlvqilcla ilwivkstra alafpfflil miplraqmsy fftaaelral
     1261 dskgsehest edepdfyeea plpg
//
LOCUS       XP_034170972            1280 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034170972
VERSION     XP_034170972.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315081.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1280
                     /product="anion exchange protein Ae2 isoform X2"
                     /calculated_mol_wt=143290
     Region          345..1279
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1280
                     /gene="Ae2"
                     /coded_by="XM_034315081.2:45..3887"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mpeaaanssg ksflqrasgk vlrwlrrslr tnhqvdghga etgpeldeem ekvfamdtge
       61 kfdvarlgsp sesagsdrdr drgppprygd rdfnqhrkrs yphphmplks irsrsmrrhl
      121 ssegsttaei gqeeengqvq tgngggpegd amdaastahd vdeetaeete nvvgsegeap
      181 iseraasgfn esptvgtprv qfenikeeel ppfqkddvts daapgtthee drkcrrhqkh
      241 ehkrhhhksr kyslqedpqw rkrsgaglpd gagmltrrvs vqpeeastlq eldiddlesh
      301 rsddprgmrr hkaahltvqi grkkeggiph dtfkkmydhs phevfvqlde lhglgeerew
      361 retarwikye edveegadrw grphvaslsf hsllnlrrcl etgvvlldle ekdvpglayr
      421 vveqmvieel ilpedrpvvm ralllrhrhv hdhdrgfrfg gkrnyssyts lqsvgleeee
      481 aareasenhn lndtkpkils snlaldtnht vvdmkeeltf tssnedlkrs hndyilkrip
      541 agaeatvvlv gavdfldqpt iafvrlaega fmpsitevti pvrfmftllg prnadldyhe
      601 igrsiatlma ntsfhkvayk anerrellsa ineflddsiv lppgdwerqa llpfnelkak
      661 seairqrkak aleekskpiq seaavkkall ageeekkppd dldplrrtkr pfgglindik
      721 rrypyylsdl tdglnsscla aaifmyfaal ctaitfgglm sdkthniigi setlisgswt
      781 givmalfstq plviigttgp lllfdeslyn fclanelefl tvrvyvgawm giialaiacv
      841 egsvlvrlft rfteeiftgl isilyivetf iklynyfvkn plldeysfip ennltsyqep
      901 lnvaemkvvs petgdtivip lekpdtlipa ynaagllinq pntalmctil clgtflgayy
      961 lrifrnshyl grsarrafgd fgvpisiivf vlidylakvk tekllvpegl sptvpgrdwi
     1021 vspvgmqkpi plwmamaciv pallvyilvf metqiselii dkkerklrkg ngyhmdivvv
     1081 clmnvgcglm gapwccaasv rslthvsavt vmsrthapgd kphivevkeq rvsallvail
     1141 vgvsvlmapl lrrvpmsvll gvflymgiss ttgvqlfdrv klffmpvkhh gtanyvrrvq
     1201 tykmhiftlv qilclailwi vkstraalaf pfflilmipl raqmsyffta aelraldskg
     1261 sehestedep dfyeeaplpg
//
LOCUS       XP_034170981            1263 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034170981
VERSION     XP_034170981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1263
                     /product="anion exchange protein Ae2 isoform X3"
                     /calculated_mol_wt=141451
     Region          345..1262
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1263
                     /gene="Ae2"
                     /coded_by="XM_034315090.2:43..3834"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mpeaaanssg ksflqrasgk vlrwlrrslr tnhqvdghga etgpeldeem ekvfamdtge
       61 kfdvarlgsp sesagsdrdr drgppprygd rdfnqhrkrs yphphmplks irsrsmrrhl
      121 ssegsttaei gqeeengqvq tgngggpegd amdaastahd vdeetaeete nvvgsegeap
      181 iseraasgfn esptvgtprv qfenikeeel ppfqkddvts daapgtthee drkcrrhqkh
      241 ehkrhhhksr kyslqedpqw rkrsgaglpd gagmltrrvs vqpeeastlq eldiddlesh
      301 rsddprgmrr hkaahltvqi grkkeggiph dtfkkmydhs phevfvqlde lhglgeerew
      361 retarwikye edveegadrw grphvaslsf hsllnlrrcl etgvvlldle ekdvpglayr
      421 vveqmvieel ilpedrpvvm ralllrhrhv hdhdrgfrfg gkrnyssyts lqnlndtkpk
      481 ilssnlaldt nhtvvdmkee ltftssnedl krshndyilk ripagaeatv vlvgavdfld
      541 qptiafvrla egafmpsite vtipvrfmft llgprnadld yheigrsiat lmantsfhkv
      601 aykanerrel lsainefldd sivlppgdwe rqallpfnel kakseairqr kakaleeksk
      661 piqseaavkk allageeekk ppddldplrr tkrpfgglin dikrrypyyl sdltdglnss
      721 claaaifmyf aalctaitfg glmsdkthni igisetlisg swtgivmalf stqplviigt
      781 tgplllfdes lynfclanel efltvrvyvg awmgiialai acvegsvlvr lftrfteeif
      841 tglisilyiv etfiklynyf vknplldeys fipennltsy qeplnvaemk vvspetgdti
      901 viplekpdtl ipaynaagll inqpntalmc tilclgtflg ayylrifrns hylgrsarra
      961 fgdfgvpisi ivfvlidyla kvktekllvp eglsptvpgr dwivspvgmq kpiplwmama
     1021 civpallvyi lvfmetqise liidkkerkl rkgngyhmdi vvvclmnvgc glmgapwcca
     1081 asvrslthvs avtvmsrtha pgdkphivev keqrvsallv ailvgvsvlm apllrrvpms
     1141 vllgvflymg issttgvqlf drvklffmpv khhgtanyvr rvqtykmhif tlvqilclai
     1201 lwivkstraa lafpfflilm iplraqmsyf ftaaelrald skgseheste depdfyeeap
     1261 lpg
//
LOCUS       XP_034170989            1268 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034170989
VERSION     XP_034170989.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1268
                     /product="anion exchange protein Ae2 isoform X4"
                     /calculated_mol_wt=142027
     Region          329..1267
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1268
                     /gene="Ae2"
                     /coded_by="XM_034315098.2:617..4423"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mentrrklsf lgfgkrvsvd ghgaetgpel deemekvfam dtgekfdvar lgspsesags
       61 drdrdrgppp rygdrdfnqh rkrsyphphm plksirsrsm rrhlssegst taeigqeeen
      121 gqvqtgnggg pegdamdaas tahdvdeeta eetenvvgse geapiseraa sgfnesptvg
      181 tprvqfenik eeelppfqkd dvtsdaapgt theedrkcrr hqkhehkrhh hksrkyslqe
      241 dpqwrkrsga glpdgagmlt rrvsvqpeea stlqeldidd leshrsddpr gmrrhkaahl
      301 tvqigrkkeg giphdtfkkm ydhsphevfv qldelhglge erewretarw ikyeedveeg
      361 adrwgrphva slsfhsllnl rrcletgvvl ldleekdvpg layrvveqmv ieelilpedr
      421 pvvmralllr hrhvhdhdrg frfggkrnys sytslqsvgl eeeeaareas enhvtqhnln
      481 dtkpkilssn laldtnhtvv dmkeeltfts snedlkrshn dyilkripag aeatvvlvga
      541 vdfldqptia fvrlaegafm psitevtipv rfmftllgpr nadldyheig rsiatlmant
      601 sfhkvaykan errellsain eflddsivlp pgdwerqall pfnelkakse airqrkakal
      661 eekskpiqse aavkkallag eeekkppddl dplrrtkrpf gglindikrr ypyylsdltd
      721 glnssclaaa ifmyfaalct aitfgglmsd kthniigise tlisgswtgi vmalfstqpl
      781 viigttgpll lfdeslynfc lanelefltv rvyvgawmgi ialaiacveg svlvrlftrf
      841 teeiftglis ilyivetfik lynyfvknpl ldeysfipen nltsyqepln vaemkvvspe
      901 tgdtiviple kpdtlipayn aagllinqpn talmctilcl gtflgayylr ifrnshylgr
      961 sarrafgdfg vpisiivfvl idylakvkte kllvpeglsp tvpgrdwivs pvgmqkpipl
     1021 wmamacivpa llvyilvfme tqiseliidk kerklrkgng yhmdivvvcl mnvgcglmga
     1081 pwccaasvrs lthvsavtvm srthapgdkp hivevkeqrv sallvailvg vsvlmapllr
     1141 rvpmsvllgv flymgisstt gvqlfdrvkl ffmpvkhhgt anyvrrvqty kmhiftlvqi
     1201 lclailwivk straalafpf flilmiplra qmsyfftaae lraldskgse hestedepdf
     1261 yeeaplpg
//
LOCUS       XP_034170998            1268 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034170998
VERSION     XP_034170998.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315107.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1268
                     /product="anion exchange protein Ae2 isoform X4"
                     /calculated_mol_wt=142027
     Region          329..1267
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1268
                     /gene="Ae2"
                     /coded_by="XM_034315107.2:316..4122"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mentrrklsf lgfgkrvsvd ghgaetgpel deemekvfam dtgekfdvar lgspsesags
       61 drdrdrgppp rygdrdfnqh rkrsyphphm plksirsrsm rrhlssegst taeigqeeen
      121 gqvqtgnggg pegdamdaas tahdvdeeta eetenvvgse geapiseraa sgfnesptvg
      181 tprvqfenik eeelppfqkd dvtsdaapgt theedrkcrr hqkhehkrhh hksrkyslqe
      241 dpqwrkrsga glpdgagmlt rrvsvqpeea stlqeldidd leshrsddpr gmrrhkaahl
      301 tvqigrkkeg giphdtfkkm ydhsphevfv qldelhglge erewretarw ikyeedveeg
      361 adrwgrphva slsfhsllnl rrcletgvvl ldleekdvpg layrvveqmv ieelilpedr
      421 pvvmralllr hrhvhdhdrg frfggkrnys sytslqsvgl eeeeaareas enhvtqhnln
      481 dtkpkilssn laldtnhtvv dmkeeltfts snedlkrshn dyilkripag aeatvvlvga
      541 vdfldqptia fvrlaegafm psitevtipv rfmftllgpr nadldyheig rsiatlmant
      601 sfhkvaykan errellsain eflddsivlp pgdwerqall pfnelkakse airqrkakal
      661 eekskpiqse aavkkallag eeekkppddl dplrrtkrpf gglindikrr ypyylsdltd
      721 glnssclaaa ifmyfaalct aitfgglmsd kthniigise tlisgswtgi vmalfstqpl
      781 viigttgpll lfdeslynfc lanelefltv rvyvgawmgi ialaiacveg svlvrlftrf
      841 teeiftglis ilyivetfik lynyfvknpl ldeysfipen nltsyqepln vaemkvvspe
      901 tgdtiviple kpdtlipayn aagllinqpn talmctilcl gtflgayylr ifrnshylgr
      961 sarrafgdfg vpisiivfvl idylakvkte kllvpeglsp tvpgrdwivs pvgmqkpipl
     1021 wmamacivpa llvyilvfme tqiseliidk kerklrkgng yhmdivvvcl mnvgcglmga
     1081 pwccaasvrs lthvsavtvm srthapgdkp hivevkeqrv sallvailvg vsvlmapllr
     1141 rvpmsvllgv flymgisstt gvqlfdrvkl ffmpvkhhgt anyvrrvqty kmhiftlvqi
     1201 lclailwivk straalafpf flilmiplra qmsyfftaae lraldskgse hestedepdf
     1261 yeeaplpg
//
LOCUS       XP_034171007            1268 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034171007
VERSION     XP_034171007.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315116.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1268
                     /product="anion exchange protein Ae2 isoform X4"
                     /calculated_mol_wt=142027
     Region          329..1267
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1268
                     /gene="Ae2"
                     /coded_by="XM_034315116.2:471..4277"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mentrrklsf lgfgkrvsvd ghgaetgpel deemekvfam dtgekfdvar lgspsesags
       61 drdrdrgppp rygdrdfnqh rkrsyphphm plksirsrsm rrhlssegst taeigqeeen
      121 gqvqtgnggg pegdamdaas tahdvdeeta eetenvvgse geapiseraa sgfnesptvg
      181 tprvqfenik eeelppfqkd dvtsdaapgt theedrkcrr hqkhehkrhh hksrkyslqe
      241 dpqwrkrsga glpdgagmlt rrvsvqpeea stlqeldidd leshrsddpr gmrrhkaahl
      301 tvqigrkkeg giphdtfkkm ydhsphevfv qldelhglge erewretarw ikyeedveeg
      361 adrwgrphva slsfhsllnl rrcletgvvl ldleekdvpg layrvveqmv ieelilpedr
      421 pvvmralllr hrhvhdhdrg frfggkrnys sytslqsvgl eeeeaareas enhvtqhnln
      481 dtkpkilssn laldtnhtvv dmkeeltfts snedlkrshn dyilkripag aeatvvlvga
      541 vdfldqptia fvrlaegafm psitevtipv rfmftllgpr nadldyheig rsiatlmant
      601 sfhkvaykan errellsain eflddsivlp pgdwerqall pfnelkakse airqrkakal
      661 eekskpiqse aavkkallag eeekkppddl dplrrtkrpf gglindikrr ypyylsdltd
      721 glnssclaaa ifmyfaalct aitfgglmsd kthniigise tlisgswtgi vmalfstqpl
      781 viigttgpll lfdeslynfc lanelefltv rvyvgawmgi ialaiacveg svlvrlftrf
      841 teeiftglis ilyivetfik lynyfvknpl ldeysfipen nltsyqepln vaemkvvspe
      901 tgdtiviple kpdtlipayn aagllinqpn talmctilcl gtflgayylr ifrnshylgr
      961 sarrafgdfg vpisiivfvl idylakvkte kllvpeglsp tvpgrdwivs pvgmqkpipl
     1021 wmamacivpa llvyilvfme tqiseliidk kerklrkgng yhmdivvvcl mnvgcglmga
     1081 pwccaasvrs lthvsavtvm srthapgdkp hivevkeqrv sallvailvg vsvlmapllr
     1141 rvpmsvllgv flymgisstt gvqlfdrvkl ffmpvkhhgt anyvrrvqty kmhiftlvqi
     1201 lclailwivk straalafpf flilmiplra qmsyfftaae lraldskgse hestedepdf
     1261 yeeaplpg
//
LOCUS       XP_034171013            1268 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034171013
VERSION     XP_034171013.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1268
                     /product="anion exchange protein Ae2 isoform X4"
                     /calculated_mol_wt=142027
     Region          329..1267
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1268
                     /gene="Ae2"
                     /coded_by="XM_034315122.2:304..4110"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mentrrklsf lgfgkrvsvd ghgaetgpel deemekvfam dtgekfdvar lgspsesags
       61 drdrdrgppp rygdrdfnqh rkrsyphphm plksirsrsm rrhlssegst taeigqeeen
      121 gqvqtgnggg pegdamdaas tahdvdeeta eetenvvgse geapiseraa sgfnesptvg
      181 tprvqfenik eeelppfqkd dvtsdaapgt theedrkcrr hqkhehkrhh hksrkyslqe
      241 dpqwrkrsga glpdgagmlt rrvsvqpeea stlqeldidd leshrsddpr gmrrhkaahl
      301 tvqigrkkeg giphdtfkkm ydhsphevfv qldelhglge erewretarw ikyeedveeg
      361 adrwgrphva slsfhsllnl rrcletgvvl ldleekdvpg layrvveqmv ieelilpedr
      421 pvvmralllr hrhvhdhdrg frfggkrnys sytslqsvgl eeeeaareas enhvtqhnln
      481 dtkpkilssn laldtnhtvv dmkeeltfts snedlkrshn dyilkripag aeatvvlvga
      541 vdfldqptia fvrlaegafm psitevtipv rfmftllgpr nadldyheig rsiatlmant
      601 sfhkvaykan errellsain eflddsivlp pgdwerqall pfnelkakse airqrkakal
      661 eekskpiqse aavkkallag eeekkppddl dplrrtkrpf gglindikrr ypyylsdltd
      721 glnssclaaa ifmyfaalct aitfgglmsd kthniigise tlisgswtgi vmalfstqpl
      781 viigttgpll lfdeslynfc lanelefltv rvyvgawmgi ialaiacveg svlvrlftrf
      841 teeiftglis ilyivetfik lynyfvknpl ldeysfipen nltsyqepln vaemkvvspe
      901 tgdtiviple kpdtlipayn aagllinqpn talmctilcl gtflgayylr ifrnshylgr
      961 sarrafgdfg vpisiivfvl idylakvkte kllvpeglsp tvpgrdwivs pvgmqkpipl
     1021 wmamacivpa llvyilvfme tqiseliidk kerklrkgng yhmdivvvcl mnvgcglmga
     1081 pwccaasvrs lthvsavtvm srthapgdkp hivevkeqrv sallvailvg vsvlmapllr
     1141 rvpmsvllgv flymgisstt gvqlfdrvkl ffmpvkhhgt anyvrrvqty kmhiftlvqi
     1201 lclailwivk straalafpf flilmiplra qmsyfftaae lraldskgse hestedepdf
     1261 yeeaplpg
//
LOCUS       XP_076546070            1264 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546070
VERSION     XP_076546070.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689955.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1264
                     /product="anion exchange protein Ae2 isoform X5"
                     /calculated_mol_wt=141562
     Region          329..1263
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1264
                     /gene="Ae2"
                     /coded_by="XM_076689955.1:617..4411"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mentrrklsf lgfgkrvsvd ghgaetgpel deemekvfam dtgekfdvar lgspsesags
       61 drdrdrgppp rygdrdfnqh rkrsyphphm plksirsrsm rrhlssegst taeigqeeen
      121 gqvqtgnggg pegdamdaas tahdvdeeta eetenvvgse geapiseraa sgfnesptvg
      181 tprvqfenik eeelppfqkd dvtsdaapgt theedrkcrr hqkhehkrhh hksrkyslqe
      241 dpqwrkrsga glpdgagmlt rrvsvqpeea stlqeldidd leshrsddpr gmrrhkaahl
      301 tvqigrkkeg giphdtfkkm ydhsphevfv qldelhglge erewretarw ikyeedveeg
      361 adrwgrphva slsfhsllnl rrcletgvvl ldleekdvpg layrvveqmv ieelilpedr
      421 pvvmralllr hrhvhdhdrg frfggkrnys sytslqsvgl eeeeaareas enhnlndtkp
      481 kilssnlald tnhtvvdmke eltftssned lkrshndyil kripagaeat vvlvgavdfl
      541 dqptiafvrl aegafmpsit evtipvrfmf tllgprnadl dyheigrsia tlmantsfhk
      601 vaykanerre llsainefld dsivlppgdw erqallpfne lkakseairq rkakaleeks
      661 kpiqseaavk kallageeek kppddldplr rtkrpfggli ndikrrypyy lsdltdglns
      721 sclaaaifmy faalctaitf gglmsdkthn iigisetlis gswtgivmal fstqplviig
      781 ttgplllfde slynfclane lefltvrvyv gawmgiiala iacvegsvlv rlftrfteei
      841 ftglisilyi vetfiklyny fvknplldey sfipennlts yqeplnvaem kvvspetgdt
      901 iviplekpdt lipaynaagl linqpntalm ctilclgtfl gayylrifrn shylgrsarr
      961 afgdfgvpis iivfvlidyl akvktekllv peglsptvpg rdwivspvgm qkpiplwmam
     1021 acivpallvy ilvfmetqis eliidkkerk lrkgngyhmd ivvvclmnvg cglmgapwcc
     1081 aasvrslthv savtvmsrth apgdkphive vkeqrvsall vailvgvsvl mapllrrvpm
     1141 svllgvflym gissttgvql fdrvklffmp vkhhgtanyv rrvqtykmhi ftlvqilcla
     1201 ilwivkstra alafpfflil miplraqmsy fftaaelral dskgsehest edepdfyeea
     1261 plpg
//
LOCUS       XP_034171019            1065 aa            linear   INV 03-SEP-2025
DEFINITION  anion exchange protein Ae2 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034171019
VERSION     XP_034171019.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315128.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1065
                     /product="anion exchange protein Ae2 isoform X6"
                     /calculated_mol_wt=120032
     Region          126..1064
                     /region_name="Band_3_cyto"
                     /note="Band 3 cytoplasmic domain; cl26877"
                     /db_xref="CDD:452680"
     CDS             1..1065
                     /gene="Ae2"
                     /coded_by="XM_034315128.2:283..3480"
                     /db_xref="GeneID:117600082"
ORIGIN      
        1 mplgrdyrqd rytmdvgrlr rferrhhhks rkyslqedpq wrkrsgaglp dgagmltrrv
       61 svqpeeastl qeldiddles hrsddprgmr rhkaahltvq igrkkeggip hdtfkkmydh
      121 sphevfvqld elhglgeere wretarwiky eedveegadr wgrphvasls fhsllnlrrc
      181 letgvvlldl eekdvpglay rvveqmviee lilpedrpvv mralllrhrh vhdhdrgfrf
      241 ggkrnyssyt slqsvgleee eaareasenh vtqhnlndtk pkilssnlal dtnhtvvdmk
      301 eeltftssne dlkrshndyi lkripagaea tvvlvgavdf ldqptiafvr laegafmpsi
      361 tevtipvrfm ftllgprnad ldyheigrsi atlmantsfh kvaykanerr ellsainefl
      421 ddsivlppgd werqallpfn elkakseair qrkakaleek skpiqseaav kkallageee
      481 kkppddldpl rrtkrpfggl indikrrypy ylsdltdgln ssclaaaifm yfaalctait
      541 fgglmsdkth niigisetli sgswtgivma lfstqplvii gttgplllfd eslynfclan
      601 elefltvrvy vgawmgiial aiacvegsvl vrlftrftee iftglisily ivetfiklyn
      661 yfvknpllde ysfipennlt syqeplnvae mkvvspetgd tiviplekpd tlipaynaag
      721 llinqpntal mctilclgtf lgayylrifr nshylgrsar rafgdfgvpi siivfvlidy
      781 lakvktekll vpeglsptvp grdwivspvg mqkpiplwma macivpallv yilvfmetqi
      841 seliidkker klrkgngyhm divvvclmnv gcglmgapwc caasvrslth vsavtvmsrt
      901 hapgdkphiv evkeqrvsal lvailvgvsv lmapllrrvp msvllgvfly mgissttgvq
      961 lfdrvklffm pvkhhgtany vrrvqtykmh iftlvqilcl ailwivkstr aalafpffli
     1021 lmiplraqms yfftaaelra ldskgsehes tedepdfyee aplpg
//
LOCUS       XP_034171037             508 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600137 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171037
VERSION     XP_034171037.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315146.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="uncharacterized protein LOC117600137 isoform X1"
                     /calculated_mol_wt=56710
     Region          <112..>298
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..508
                     /gene="LOC117600137"
                     /coded_by="XM_034315146.2:1684..3210"
                     /db_xref="GeneID:117600137"
ORIGIN      
        1 matttgryga memrhsksed llgviggsrs pspsvalpst aseddliaas alheatdttv
       61 kpacpllssr vagaagipad ridpedsird ivtendlyrf vlfkrhydky valaakyeea
      121 kgvayyleer yhevkaerdk leearrnlek rlegceaelr gkedelflql erslrleeev
      181 eraknerdsc laardrldrq revalrrlqm qlaqneitrq tlerarqdvv rqatviraer
      241 dalerenevl keklrveqge lgeerrrree gvaaltreti tlrhaarhlr aatlhatacr
      301 rrrrcsvcly arrtfaeidd yrddgnlfkc lqvplqdlrt wlrpnatstt ptsrgqrtns
      361 pladreisfi ddssvdssag gsnvsnssss snsasddeay pstpvaeisl ssanssappt
      421 arafssdsgf sseigdrrsr cyenggssts sssgksrkis eslscsepde qstggftrsr
      481 wtssfrkllg rkpktkstpd rtsrrned
//
LOCUS       XP_034171048             413 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600137 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171048
VERSION     XP_034171048.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315157.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..413
                     /product="uncharacterized protein LOC117600137 isoform X2"
                     /calculated_mol_wt=46919
     Region          <17..>197
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..413
                     /gene="LOC117600137"
                     /coded_by="XM_034315157.2:351..1592"
                     /db_xref="GeneID:117600137"
ORIGIN      
        1 mterfvlfkr hydkyvalaa kyeeakgvay yleeryhevk aerdkleear rnlekrlegc
       61 eaelrgkede lflqlerslr leeeverakn erdsclaard rldrqreval rrlqmqlaqn
      121 eitrqtlera rqdvvrqatv iraerdaler enevlkeklr veqgelgeer rrreegvaal
      181 tretitlrha arhlraatlh atacrrrrrc svclyarrtf aeiddyrddg nlfkclqvpl
      241 qdlrtwlrpn atsttptsrg qrtnspladr eisfiddssv dssaggsnvs nsssssnsas
      301 ddeaypstpv aeislssans sapptarafs sdsgfsseig drrsrcyeng gsstssssgk
      361 srkiseslsc sepdeqstgg ftrsrwtssf rkllgrkpkt kstpdrtsrr ned
//
LOCUS       XP_034171063             327 aa            linear   INV 03-SEP-2025
DEFINITION  proto-oncogene serine/threonine-protein kinase mos [Osmia lignaria
            lignaria].
ACCESSION   XP_034171063
VERSION     XP_034171063.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="proto-oncogene serine/threonine-protein kinase
                     mos"
                     /calculated_mol_wt=36162
     Region          75..324
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(85..88,91,93,104,106,132,148..151,192,196..197,199,
                     209..210)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..327
                     /gene="Mos"
                     /coded_by="XM_034315172.2:452..1435"
                     /db_xref="GeneID:117600143"
ORIGIN      
        1 mycscykysc kmaspqrlat klynisprll knvekrqfls pttparntyk rnienkdqls
       61 pfnldtpnrr kilqdglpak rgkvlgsggf gtvykafykg dqvaakmmqt ekcssalsse
      121 khasflkhsn ivkvliveqg vslsliamel cgptlqdrld etaltkferv silrdvtsal
      181 qfchnagivh advkpknilm sadglskltd fgssvsvgep seitefhgtp gytapevlkg
      241 nrptpaadiy slgivawqmi srklpfaglh shtiiyisak gsrpkdddsd defngvyktl
      301 yrkmwsqnai drpttneiis kintmia
//
LOCUS       XP_034171077             172 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoside diphosphate kinase [Osmia lignaria lignaria].
ACCESSION   XP_034171077
VERSION     XP_034171077.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315186.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..172
                     /product="nucleoside diphosphate kinase"
                     /calculated_mol_wt=19454
     Region          24..153
                     /region_name="NDPk_I"
                     /note="Nucleoside diphosphate kinase Group I
                     (NDPk_I)-like: NDP kinase domains are present in a large
                     family of structurally and functionally conserved proteins
                     from bacteria to humans that generally catalyze the
                     transfer of gamma-phosphates of a nucleoside...; cd04413"
                     /db_xref="CDD:239876"
     Site            order(31,71,79,107,113,124,134,137,139..140,148)
                     /site_type="active"
                     /db_xref="CDD:239876"
     Site            order(35,40..42,45,48,57..59)
                     /site_type="other"
                     /note="multimer interface [polypeptide binding]"
                     /db_xref="CDD:239876"
     CDS             1..172
                     /gene="awd"
                     /coded_by="XM_034315186.2:97..615"
                     /db_xref="GeneID:117600147"
ORIGIN      
        1 mvflcsvlvl fylvyksvma enkertfimi kpdgvqrglv gkiiqrfeek gfklvamkmv
       61 wptedllkkh ysdlasrpff pglvkymssg pvvpmvwegl nsvktgrvml getnpkdsap
      121 gtirgdyciq vgrniihgsd svesatkein lwfgepkdvv dwatwaekwi ye
//
LOCUS       XP_034171091             185 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600150 [Osmia lignaria lignaria].
ACCESSION   XP_034171091
VERSION     XP_034171091.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315200.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..185
                     /product="uncharacterized protein LOC117600150"
                     /calculated_mol_wt=21169
     CDS             1..185
                     /gene="LOC117600150"
                     /coded_by="XM_034315200.2:500..1057"
                     /db_xref="GeneID:117600150"
ORIGIN      
        1 mdrtfvdeet gesinvpasd ceiqrlissv nnaetgepei nvigfemtkn rkirfqipke
       61 vketlstkdg slkksenlme lepvpvgasf gqinpvclff lavlcfrwfl pvlmifevsl
      121 hvwahrknka lknasvyfrs pfhaissefc alcqnetcmd rvgkmqqirm hkflrqcnym
      181 krvvt
//
LOCUS       XP_034171099             185 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600150 [Osmia lignaria lignaria].
ACCESSION   XP_034171099
VERSION     XP_034171099.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315208.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..185
                     /product="uncharacterized protein LOC117600150"
                     /calculated_mol_wt=21169
     CDS             1..185
                     /gene="LOC117600150"
                     /coded_by="XM_034315208.2:497..1054"
                     /db_xref="GeneID:117600150"
ORIGIN      
        1 mdrtfvdeet gesinvpasd ceiqrlissv nnaetgepei nvigfemtkn rkirfqipke
       61 vketlstkdg slkksenlme lepvpvgasf gqinpvclff lavlcfrwfl pvlmifevsl
      121 hvwahrknka lknasvyfrs pfhaissefc alcqnetcmd rvgkmqqirm hkflrqcnym
      181 krvvt
//
LOCUS       XP_034171105             185 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600150 [Osmia lignaria lignaria].
ACCESSION   XP_034171105
VERSION     XP_034171105.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315214.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..185
                     /product="uncharacterized protein LOC117600150"
                     /calculated_mol_wt=21169
     CDS             1..185
                     /gene="LOC117600150"
                     /coded_by="XM_034315214.2:721..1278"
                     /db_xref="GeneID:117600150"
ORIGIN      
        1 mdrtfvdeet gesinvpasd ceiqrlissv nnaetgepei nvigfemtkn rkirfqipke
       61 vketlstkdg slkksenlme lepvpvgasf gqinpvclff lavlcfrwfl pvlmifevsl
      121 hvwahrknka lknasvyfrs pfhaissefc alcqnetcmd rvgkmqqirm hkflrqcnym
      181 krvvt
//
LOCUS       XP_034171114             185 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600150 [Osmia lignaria lignaria].
ACCESSION   XP_034171114
VERSION     XP_034171114.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315223.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..185
                     /product="uncharacterized protein LOC117600150"
                     /calculated_mol_wt=21169
     CDS             1..185
                     /gene="LOC117600150"
                     /coded_by="XM_034315223.2:375..932"
                     /db_xref="GeneID:117600150"
ORIGIN      
        1 mdrtfvdeet gesinvpasd ceiqrlissv nnaetgepei nvigfemtkn rkirfqipke
       61 vketlstkdg slkksenlme lepvpvgasf gqinpvclff lavlcfrwfl pvlmifevsl
      121 hvwahrknka lknasvyfrs pfhaissefc alcqnetcmd rvgkmqqirm hkflrqcnym
      181 krvvt
//
LOCUS       XP_034171126             126 aa            linear   INV 03-SEP-2025
DEFINITION  lipopolysaccharide-induced tumor necrosis factor-alpha factor
            homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034171126
VERSION     XP_034171126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..126
                     /product="lipopolysaccharide-induced tumor necrosis
                     factor-alpha factor homolog isoform X1"
                     /calculated_mol_wt=13361
     Region          57..122
                     /region_name="zf-LITAF-like"
                     /note="LITAF-like zinc ribbon domain; pfam10601"
                     /db_xref="CDD:463165"
     CDS             1..126
                     /gene="LOC117600169"
                     /coded_by="XM_034315235.2:152..532"
                     /db_xref="GeneID:117600169"
ORIGIN      
        1 msvrkapdim skemsappap gfipsptpgf vpppappsyt dgqpnivvvg apqfdsesqr
       61 ltcphchani steiqtqans kthlfalllc vtglwccapc pycidscqtk khycpackay
      121 lgesaq
//
LOCUS       XP_034171129             117 aa            linear   INV 03-SEP-2025
DEFINITION  lipopolysaccharide-induced tumor necrosis factor-alpha factor
            homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034171129
VERSION     XP_034171129.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315238.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..117
                     /product="lipopolysaccharide-induced tumor necrosis
                     factor-alpha factor homolog isoform X2"
                     /calculated_mol_wt=12363
     Region          48..113
                     /region_name="zf-LITAF-like"
                     /note="LITAF-like zinc ribbon domain; pfam10601"
                     /db_xref="CDD:463165"
     CDS             1..117
                     /gene="LOC117600169"
                     /coded_by="XM_034315238.2:20..373"
                     /db_xref="GeneID:117600169"
ORIGIN      
        1 mskemsappa pgfipsptpg fvpppappsy tdgqpnivvv gapqfdsesq rltcphchan
       61 isteiqtqan skthlfalll cvtglwccap cpycidscqt kkhycpacka ylgesaq
//
LOCUS       XP_076546071             109 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305639 [Osmia lignaria lignaria].
ACCESSION   XP_076546071
VERSION     XP_076546071.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689956.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..109
                     /product="uncharacterized protein LOC143305639"
                     /calculated_mol_wt=11956
     Region          <6..54
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     CDS             1..109
                     /gene="LOC143305639"
                     /coded_by="XM_076689956.1:1..330"
                     /db_xref="GeneID:143305639"
ORIGIN      
        1 mkrmvqwvea cdnpnlslls peqlksqvic sshfeqkyim mkkltmsavp tlnlplseaa
       61 sikieqqdtr meipeeeeec kpdctggids ksgimghvdf tallgpsgd
//
LOCUS       XP_034171142              91 aa            linear   INV 03-SEP-2025
DEFINITION  U6 snRNA-associated Sm-like protein LSm5 [Osmia lignaria lignaria].
ACCESSION   XP_034171142
VERSION     XP_034171142.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315251.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..91
                     /product="U6 snRNA-associated Sm-like protein LSm5"
                     /calculated_mol_wt=9786
     Region          11..86
                     /region_name="LSm5"
                     /note="Like-Sm protein 5; cd01732"
                     /db_xref="CDD:212479"
     Site            order(18,21..22,27,29..31,34,36,41..44,47..48,50,71..83)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:212479"
     Site            order(26..32,34..46,48..52)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212479"
     Site            order(44,46,75)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212479"
     Site            71..82
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212479"
     CDS             1..91
                     /gene="LOC117600183"
                     /coded_by="XM_034315251.2:97..372"
                     /db_xref="GeneID:117600183"
ORIGIN      
        1 mtssvstnps tllplelvdk cigsrihiim kndkeivgtl lgfddfvnml ledvteseat
       61 pegrrvtkld qillngsnit mlvpggempd t
//
LOCUS       XP_034171057             232 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600119 [Osmia lignaria lignaria].
ACCESSION   XP_034171057
VERSION     XP_034171057.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315166.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171057.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..232
                     /product="uncharacterized protein LOC117600119"
                     /calculated_mol_wt=23995
     Region          40..109
                     /region_name="Linker_histone"
                     /note="linker histone H1 and H5 family; pfam00538"
                     /db_xref="CDD:459846"
     CDS             1..232
                     /gene="LOC117600119"
                     /coded_by="XM_034315166.2:44..742"
                     /db_xref="GeneID:117600119"
ORIGIN      
        1 mearnssgnt atvensvakp saakksvksk tksqrtkswh pstadmvnaa ikaledrkgs
       61 slqaikkyia ttykvdgekm apfikrylkt avtagavvqt tgkgasgsfk lstsskgses
      121 kskskrlvkr avsaaaekka aekpaspkka tapkkapvvk kpaaaakkqs sskkaaktaa
      181 akitesvkqk aapppknlsk akkatkapaa ktktpkpkta kspkiaraaa kk
//
LOCUS       XP_076546072             136 aa            linear   INV 03-SEP-2025
DEFINITION  histone H3 [Osmia lignaria lignaria].
ACCESSION   XP_076546072
VERSION     XP_076546072.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689957.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="histone H3"
                     /calculated_mol_wt=15257
     Region          1..136
                     /region_name="PTZ00018"
                     /note="histone H3; Provisional"
                     /db_xref="CDD:185400"
     Site            order(40..48,50,64..67,70,73,84..87,117..119,121)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467036"
     Site            order(45,48,51..52,55..56,58,60,62..63,67..68,70..72,
                     74..75,77,79,82..86,88..89,92..93,96..98,100..102,
                     104..106,109,118..122,126,129)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     Site            order(49,52..53,56..57,59,95,99,102,109,113,118)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     Site            order(107,110..111,114..115,117,123..124,127..128,
                     130..132)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     CDS             1..136
                     /gene="LOC117600239"
                     /coded_by="XM_076689957.1:27..437"
                     /db_xref="GeneID:117600239"
ORIGIN      
        1 martkqtark stggkaprkq latkaarksa patggvkkph ryrpgtvalr eirryqkste
       61 llirklpfqr lvreiaqdfk tdlrfqssav malqeaseay lvglfedtnl caihakrvti
      121 mpkdiqlarr irgera
//
LOCUS       XP_076546073             124 aa            linear   INV 03-SEP-2025
DEFINITION  histone H2A-like [Osmia lignaria lignaria].
ACCESSION   XP_076546073
VERSION     XP_076546073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689958.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="histone H2A-like"
                     /calculated_mol_wt=13162
     Region          16..124
                     /region_name="PTZ00017"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:185399"
     CDS             1..124
                     /gene="LOC117600439"
                     /coded_by="XM_076689958.1:39..413"
                     /db_xref="GeneID:117600439"
ORIGIN      
        1 msgrgkggka kgkaksrssr aglqfpvgri hrllrkgnya ervgagapvy laavmeylaa
       61 evlelagnaa rdnkktriip rhlqlairnd eelnkllsgv tiaqggvlpn iqavllpkkt
      121 dkka
//
LOCUS       XP_034171788             123 aa            linear   INV 03-SEP-2025
DEFINITION  histone H2B [Osmia lignaria lignaria].
ACCESSION   XP_034171788
VERSION     XP_034171788.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315897.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="histone H2B"
                     /calculated_mol_wt=13563
     Region          33..120
                     /region_name="HFD_H2B"
                     /note="histone-fold domain found in histone H2B and
                     similar proteins; cd22910"
                     /db_xref="CDD:467035"
     Site            order(33,35,37..39,41..43,46..53,55..56,59..60,63..64,
                     66..68,70..72,76,86..87,89,92..93,96,99..101,104,107..109,
                     111..112,115..116,119..120)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467035"
     Site            order(36..38,40..41,44..46,51..53,57,93,97,106..107,
                     109..110,113..114,116..117,120)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467035"
     Site            order(59,62..63,65..66,69,74,77,80..82,90..91,94,98..99)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467035"
     CDS             1..123
                     /gene="LOC117600447"
                     /coded_by="XM_034315897.2:45..416"
                     /db_xref="GeneID:117600447"
ORIGIN      
        1 mppkasgkav kkagkaqkni sktdkkkkrr rkesyaiyiy kvlkqvhpdt gisskamsim
       61 nsfvndvfer iaaeasrlah ynkrstitsr eiqtavrlll pgelakhavs egtkavtkyt
      121 ssk
//
LOCUS       XP_034172482             103 aa            linear   INV 03-SEP-2025
DEFINITION  histone H4 [Osmia lignaria lignaria].
ACCESSION   XP_034172482
VERSION     XP_034172482.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316591.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..103
                     /product="histone H4"
                     /calculated_mol_wt=11236
     Region          1..103
                     /region_name="PLN00035"
                     /note="histone H4; Provisional"
                     /db_xref="CDD:177669"
     CDS             1..103
                     /gene="LOC117600738"
                     /coded_by="XM_034316591.2:37..348"
                     /db_xref="GeneID:117600738"
ORIGIN      
        1 msgrgkggkg lgkggakrhr kvlrdniqgi tkpairrlar rggvkrisgl iyeetrgvlk
       61 vflenvirda vtytehakrk tvtamdvvya lkrqgrtlyg fgg
//
LOCUS       XP_034171801             103 aa            linear   INV 03-SEP-2025
DEFINITION  histone H4 [Osmia lignaria lignaria].
ACCESSION   XP_034171801
VERSION     XP_034171801.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315910.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..103
                     /product="histone H4"
                     /calculated_mol_wt=11250
     Region          1..103
                     /region_name="PLN00035"
                     /note="histone H4; Provisional"
                     /db_xref="CDD:177669"
     CDS             1..103
                     /gene="LOC117600455"
                     /coded_by="XM_034315910.2:13..324"
                     /db_xref="GeneID:117600455"
ORIGIN      
        1 mtgrgkggkg lgkggakrhr kvlrdniqgi tkpairrlar rggvkrisgl iyeetrgvlk
       61 vflenvirda vtytehakrk tvtamdvvya lkrqgrtlyg fgg
//
LOCUS       XP_034171268             476 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600240 [Osmia lignaria lignaria].
ACCESSION   XP_034171268
VERSION     XP_034171268.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315377.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="uncharacterized protein LOC117600240"
                     /calculated_mol_wt=51567
     Region          12..103
                     /region_name="PTB_FRS2"
                     /note="Fibroblast growth factor receptor substrate 2
                     phosphotyrosine-binding domain; cd01202"
                     /db_xref="CDD:269913"
     Site            order(22..25,30,33,44,46..47,52,54,57,59..66,68,70..71,79,
                     82..86,95,99,102)
                     /site_type="other"
                     /note="phosphopeptide binding site [polypeptide binding]"
                     /db_xref="CDD:269913"
     CDS             1..476
                     /gene="LOC117600240"
                     /coded_by="XM_034315377.2:170..1600"
                     /db_xref="GeneID:117600240"
ORIGIN      
        1 mgcvnsrtdi ndlhpnifqv mnvddsgnli tpgrlevtes divlyqrgkq pikwplrclr
       61 rygydaeifs fesgrrcstg pgiyafkcrr aahlfnlvqt niqvcnnsgd dtisrelpva
      121 shpgqtvtrv tvpvepnyld pilsrtnnrt gsrfahnqqn gvgrldsigs stglispqgn
      181 isspssppvl pppppplpqp hpsslyvnee vlsslpleme hnnnkslrra iqrsctvsns
      241 isssgsvsle qssihkssev tsqtksplsv apymnvdins disslspths iseatqfkkd
      301 knvnnecgha ymnispgqek seslsgrqrp splpciqsei eegvrhcyan lepseieslr
      361 krfsgvsvae ksplppstpt ggpirevnya vldldtkdvp ttspldgsst ftasppespn
      421 klqkgyatid fnktaalshs vnpnlvndne gsrktrhnst isdlaasgrr sssvse
//
LOCUS       XP_034171282             318 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein 330 homolog Noa36 [Osmia lignaria lignaria].
ACCESSION   XP_034171282
VERSION     XP_034171282.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315391.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171282.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="zinc finger protein 330 homolog Noa36"
                     /calculated_mol_wt=36344
     Region          1..252
                     /region_name="NOA36"
                     /note="NOA36 protein; pfam06524"
                     /db_xref="CDD:368953"
     CDS             1..318
                     /gene="Noa36"
                     /coded_by="XM_034315391.2:114..1070"
                     /db_xref="GeneID:117600244"
ORIGIN      
        1 mpkkktgqrr kaekqklrqk eiraakdqin lakfpcnavm ecdkcgkkqk srafcyfcqn
       61 vqrlpmcahc gkikcmlktg dcvvrhpgif ttglgmvgai cdhceawvch grrcltthac
      121 icplmdavcq ecergvwdhg grifrcsfcd cflceddqfe hqascqvlea enfkcqscnr
      181 lgqysclrck tcycedhvrr kgfkydknkp ipcpkcgfet sqtkdlsmst rshkfgrqnq
      241 sgmyesddes gyscygnqsq yhvsenytgs dqenedeeed dydgdededd dddeqevene
      301 ssseneenen ntlpkcnh
//
LOCUS       XP_034171294             179 aa            linear   INV 03-SEP-2025
DEFINITION  signal peptidase complex subunit Spase22-23 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171294
VERSION     XP_034171294.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315403.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..179
                     /product="signal peptidase complex subunit Spase22-23"
                     /calculated_mol_wt=20178
     Region          1..173
                     /region_name="SPC22"
                     /note="Signal peptidase subunit; pfam04573"
                     /db_xref="CDD:461357"
     CDS             1..179
                     /gene="Spase22-23"
                     /coded_by="XM_034315403.2:305..844"
                     /db_xref="GeneID:117600249"
ORIGIN      
        1 mhtvftrgna ilaytlsvaa cltfccflst vfidyranat lntvivavkn vpdysasrek
       61 ndlgyltfdl qtdltplfnw nvkqlflylt aeyqtenndf nqvvlwdkiv lrgdnavldf
      121 kniytkyyfw ddgnglrgnk nvtltlswni ipnagilpsv nalgshtfaf pseytslrv
//
LOCUS       XP_034171307            1355 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 23 [Osmia lignaria lignaria].
ACCESSION   XP_034171307
VERSION     XP_034171307.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315416.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1355
                     /product="mediator complex subunit 23"
                     /calculated_mol_wt=154919
     Region          4..1319
                     /region_name="Med23"
                     /note="Mediator complex subunit 23; pfam11573"
                     /db_xref="CDD:463299"
     CDS             1..1355
                     /gene="MED23"
                     /coded_by="XM_034315416.2:233..4300"
                     /db_xref="GeneID:117600257"
ORIGIN      
        1 mtseaqitni vndilkveai eeafscvlvy hpnsenekla twqteltsam snlpkeqqes
       61 avrqflsmaa amtnhrrlql llsllenlvt snvlparlvc eciltcdklq yqledfwvec
      121 fvlirhiigg vdykgvreim kgckekaqti parlnasiqp qlkalenvie yifdrnacll
      181 pgyfivneiq kaypdgknwp hwklakllsn fvesfrntaq mvsivghskm lpvvehtgya
      241 dlinpwvldt ttlkfslkgn lpydedllks qtgllryvle qpysrdmvcs mlglqkqqkq
      301 rcvaleeqlv elvilamera eneslpaegt dgtvanhyvw lhlssqliyf vlfsyacfps
      361 ivmaihdklt grelrkgrdh lmwvllqfis gsiqrnplsn flpvlklydl lypekeplpv
      421 pdytqalcth qmaitciwih llkkaqsehs nihrpiphtl klhheflqhl vmpntslcmg
      481 sdyriallcn aystnqeyfg rpmaalvdti lgtqkgqqqq pmqtlqnnaa lasgpttpls
      541 msildsltvh skmslihsiv thviklaqsk snmalapalv etysrllvyt eieslgikgf
      601 isqllptvfk shawgtlytl lemfsyrmhh iqphyrvqll shlhslaavp qtnqtqlhlc
      661 vestalrlit glgsaevqpq lsrflsepkt lvsaeseeln ralvltlars mhvtgtgsds
      721 hsgtwckell ntimqntphs wanhtlkcfp pvlseffqqn svpkenkqqi kkaveeeyrn
      781 wasmsnendi iahfsvpgtp plflcllwkm ifetdrispi aykileriga ralsahlrkf
      841 cdylvfefan svggqhvnkc vdtindmiwk ynivtidrli lclvlrtheg seaqvcffii
      901 qllllkaief rnrvqefvke nspehwkqsn whekhlafhr kfpekfapeg imeqtsggps
      961 qyqslpvyfg nvclrflpvf divvhrylei pavaksleal lehlgclykf hdrpvtylyn
     1021 tlhyyerklr dnpplkrrlv favlgslrei rapgwalsea yqmymtrstd dvvtwmpeld
     1081 yyirlvqriv etmsgmahfp atdwrfnefp npaahalyvt cvelmavpva pnlvanslld
     1141 vvakgytvip sdeihlwinc vglllaalpe cywsalhdrl vetisspgla nwqynnltpf
     1201 qmfnfnnthn sllenkysym lalahsvwhh agvgqittmp qfikenlqpv vnseeqliya
     1261 chligptlar fnaerpncvv elavclyeml eqvdrlqshl kymdpvcdll yhikymfvgd
     1321 mmknevecii rrlrpalqmr lrfiahinid eiqss
//
LOCUS       XP_034171319            1321 aa            linear   INV 03-SEP-2025
DEFINITION  rhoGAP_ARAP and RA_ARAPs domain-containing protein RhoGAP15B
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034171319
VERSION     XP_034171319.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315428.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171319.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1321
                     /product="rhoGAP_ARAP and RA_ARAPs domain-containing
                     protein RhoGAP15B isoform X1"
                     /calculated_mol_wt=148267
     Region          655..756
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          874..1058
                     /region_name="RhoGAP_ARAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in ARAPs. ARAPs (also known
                     as centaurin deltas) contain, besides the RhoGAP domain,
                     an Arf GAP, ankyrin repeat ras-associating, and PH
                     domains. Since their ArfGAP activity...; cd04385"
                     /db_xref="CDD:239850"
     Site            order(911,950,954,1023,1026..1027,1048)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239850"
     Site            911
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239850"
     Region          1094..1188
                     /region_name="RA_ARAPs"
                     /note="Ras-associating (RA) domain found in Arf-GAP with
                     Rho-GAP domain, ANK repeat and PH domain-containing
                     proteins ARAP1, ARAP2, ARAP3, and similar proteins;
                     cd17113"
                     /db_xref="CDD:340633"
     CDS             1..1321
                     /gene="RhoGAP15B"
                     /coded_by="XM_034315428.2:317..4282"
                     /db_xref="GeneID:117600266"
ORIGIN      
        1 myrtniasrs kcstlrrdie neepstnfat dtnhvaqllr swtirlktss elekypveme
       61 vkpipkprst lserpvpapr klppalpatr vythsessqs eksddsksip dtrsstntef
      121 frnlstssrq lkdeisermt ikgravisst inasirleks vknlltrrlt tlnqdellrd
      181 nstdskklks pvendrcasl paddifsnis fysplnsnlr svrneedlsg mrhsppppvy
      241 pppplpdesi ydelqsvtsg ssrydtisdk verdfpgsfd llnlvlnqts dsdqslnlsd
      301 vnvalpydks dsikrlsrsd swtfydtapa sksegvdeld rissaeedil dkeipildrt
      361 ssasnesqas iqnslyenls yrkvqeeket mpavtenrqq ssksllfefd pfartseenv
      421 ysnyenndrm lletllssnd tpsssgsild sqevteneee qeevenedva ehprilpvpp
      481 eppkrfdslp kneydevtet lstdksttgk npallpklah lvtrkqpavp prksagkrsn
      541 ntssaasktv tsttndgstp vttttsvtst nitketsnss sgktteeprr vgmiqklrkl
      601 rhessshgik pnvisfvksg skllsrnrdh sgtvsnanlk lerpkvdglq npakhsgtvy
      661 rngvgierak dlvprlavls dqklsfytdk gmstlkeviq ldtvysihll qdvkmvdget
      721 vyciaisgeg rpsvhvfyak givewriwaq rileaittvf ptkytaedlr ragwaylkeg
      781 vtgvwfpawv llhqrtliyt kslepsmgvs fgkldlrkar civlreqegp npgmgsipvv
      841 vvdaggsgal hvaapgvheg sawrhalyqa atncgpaleq qqltqdnvpv ildkcinfiy
      901 vhgimtegiy rrsgsnsavv klleafrrda watqitrgay tehdvatvlr rflrdlpnpl
      961 fpadihdrlc ftsennkede kvstyrklls mlgavpaatl rrilahlhcl sqqssrnlmt
     1021 cenlsaiwgp tlmrageksa ekwnraetrv igdliklypk lyqltaadla keakilevle
     1081 khhvsnngpr gapsgdlkiw iyifsrdgec vnvtigphkt aydvcqelve ktnlsahelc
     1141 leeytlsgal erplhhserv letvarwgyw dpedrkdnvl ilkkdqlykd iaplvtppmt
     1201 tagelkfadk ksknfknylf efshaklycy kdkfcsnkly ewkiediiwy vghepkrnpq
     1261 tgwsitfiak ntkptrckes pffgntlags lkdeqykwla amtfaeyqln irppsinlme
     1321 p
//
LOCUS       XP_034171339            1296 aa            linear   INV 03-SEP-2025
DEFINITION  rhoGAP_ARAP and RA_ARAPs domain-containing protein RhoGAP15B
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034171339
VERSION     XP_034171339.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315448.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171339.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1296
                     /product="rhoGAP_ARAP and RA_ARAPs domain-containing
                     protein RhoGAP15B isoform X2"
                     /calculated_mol_wt=145388
     Region          630..731
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          849..1033
                     /region_name="RhoGAP_ARAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in ARAPs. ARAPs (also known
                     as centaurin deltas) contain, besides the RhoGAP domain,
                     an Arf GAP, ankyrin repeat ras-associating, and PH
                     domains. Since their ArfGAP activity...; cd04385"
                     /db_xref="CDD:239850"
     Site            order(886,925,929,998,1001..1002,1023)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239850"
     Site            886
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239850"
     Region          1069..1163
                     /region_name="RA_ARAPs"
                     /note="Ras-associating (RA) domain found in Arf-GAP with
                     Rho-GAP domain, ANK repeat and PH domain-containing
                     proteins ARAP1, ARAP2, ARAP3, and similar proteins;
                     cd17113"
                     /db_xref="CDD:340633"
     CDS             1..1296
                     /gene="RhoGAP15B"
                     /coded_by="XM_034315448.2:74..3964"
                     /db_xref="GeneID:117600266"
ORIGIN      
        1 mdygkdtnhv aqllrswtir lktsseleky pvemevkpip kprstlserp vpaprklppa
       61 lpatrvyths essqseksdd sksipdtrss tnteffrnls tssrqlkdei sermtikgra
      121 visstinasi rleksvknll trrlttlnqd ellrdnstds kklkspvend rcaslpaddi
      181 fsnisfyspl nsnlrsvrne edlsgmrhsp pppvyppppl pdesiydelq svtsgssryd
      241 tisdkverdf pgsfdllnlv lnqtsdsdqs lnlsdvnval pydksdsikr lsrsdswtfy
      301 dtapaskseg vdeldrissa eedildkeip ildrtssasn esqasiqnsl yenlsyrkvq
      361 eeketmpavt enrqqssksl lfefdpfart seenvysnye nndrmlletl lssndtpsss
      421 gsildsqevt eneeeqeeve nedvaehpri lpvppeppkr fdslpkneyd evtetlstdk
      481 sttgknpall pklahlvtrk qpavpprksa gkrsnntssa asktvtsttn dgstpvtttt
      541 svtstnitke tsnsssgktt eeprrvgmiq klrklrhess shgikpnvis fvksgsklls
      601 rnrdhsgtvs nanlklerpk vdglqnpakh sgtvyrngvg ierakdlvpr lavlsdqkls
      661 fytdkgmstl keviqldtvy sihllqdvkm vdgetvycia isgegrpsvh vfyakgivew
      721 riwaqrilea ittvfptkyt aedlrragwa ylkegvtgvw fpawvllhqr tliytkslep
      781 smgvsfgkld lrkarcivlr eqegpnpgmg sipvvvvdag gsgalhvaap gvhegsawrh
      841 alyqaatncg paleqqqltq dnvpvildkc infiyvhgim tegiyrrsgs nsavvkllea
      901 frrdawatqi trgaytehdv atvlrrflrd lpnplfpadi hdrlcftsen nkedekvsty
      961 rkllsmlgav paatlrrila hlhclsqqss rnlmtcenls aiwgptlmra geksaekwnr
     1021 aetrvigdli klypklyqlt aadlakeaki levlekhhvs nngprgapsg dlkiwiyifs
     1081 rdgecvnvti gphktaydvc qelvektnls ahelcleeyt lsgalerplh hservletva
     1141 rwgywdpedr kdnvlilkkd qlykdiaplv tppmttagel kfadkksknf knylfefsha
     1201 klycykdkfc snklyewkie diiwyvghep krnpqtgwsi tfiakntkpt rckespffgn
     1261 tlagslkdeq ykwlaamtfa eyqlnirpps inlmep
//
LOCUS       XP_034171349            1263 aa            linear   INV 03-SEP-2025
DEFINITION  rhoGAP_ARAP and RA_ARAPs domain-containing protein RhoGAP15B
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034171349
VERSION     XP_034171349.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315458.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171349.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1263
                     /product="rhoGAP_ARAP and RA_ARAPs domain-containing
                     protein RhoGAP15B isoform X3"
                     /calculated_mol_wt=141496
     Region          597..698
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          816..1000
                     /region_name="RhoGAP_ARAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in ARAPs. ARAPs (also known
                     as centaurin deltas) contain, besides the RhoGAP domain,
                     an Arf GAP, ankyrin repeat ras-associating, and PH
                     domains. Since their ArfGAP activity...; cd04385"
                     /db_xref="CDD:239850"
     Site            order(853,892,896,965,968..969,990)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239850"
     Site            853
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239850"
     Region          1036..1130
                     /region_name="RA_ARAPs"
                     /note="Ras-associating (RA) domain found in Arf-GAP with
                     Rho-GAP domain, ANK repeat and PH domain-containing
                     proteins ARAP1, ARAP2, ARAP3, and similar proteins;
                     cd17113"
                     /db_xref="CDD:340633"
     CDS             1..1263
                     /gene="RhoGAP15B"
                     /coded_by="XM_034315458.2:222..4013"
                     /db_xref="GeneID:117600266"
ORIGIN      
        1 mevkpipkpr stlserpvpa prklppalpa trvythsess qseksddsks ipdtrsstnt
       61 effrnlstss rqlkdeiser mtikgravis stinasirle ksvknlltrr lttlnqdell
      121 rdnstdskkl kspvendrca slpaddifsn isfysplnsn lrsvrneedl sgmrhspppp
      181 vypppplpde siydelqsvt sgssrydtis dkverdfpgs fdllnlvlnq tsdsdqslnl
      241 sdvnvalpyd ksdsikrlsr sdswtfydta pasksegvde ldrissaeed ildkeipild
      301 rtssasnesq asiqnslyen lsyrkvqeek etmpavtenr qqssksllfe fdpfartsee
      361 nvysnyennd rmlletllss ndtpsssgsi ldsqevtene eeqeevened vaehprilpv
      421 ppeppkrfds lpkneydevt etlstdkstt gknpallpkl ahlvtrkqpa vpprksagkr
      481 snntssaask tvtsttndgs tpvttttsvt stnitketsn sssgktteep rrvgmiqklr
      541 klrhessshg ikpnvisfvk sgskllsrnr dhsgtvsnan lklerpkvdg lqnpakhsgt
      601 vyrngvgier akdlvprlav lsdqklsfyt dkgmstlkev iqldtvysih llqdvkmvdg
      661 etvyciaisg egrpsvhvfy akgivewriw aqrileaitt vfptkytaed lrragwaylk
      721 egvtgvwfpa wvllhqrtli ytkslepsmg vsfgkldlrk arcivlreqe gpnpgmgsip
      781 vvvvdaggsg alhvaapgvh egsawrhaly qaatncgpal eqqqltqdnv pvildkcinf
      841 iyvhgimteg iyrrsgsnsa vvklleafrr dawatqitrg aytehdvatv lrrflrdlpn
      901 plfpadihdr lcftsennke dekvstyrkl lsmlgavpaa tlrrilahlh clsqqssrnl
      961 mtcenlsaiw gptlmragek saekwnraet rvigdlikly pklyqltaad lakeakilev
     1021 lekhhvsnng prgapsgdlk iwiyifsrdg ecvnvtigph ktaydvcqel vektnlsahe
     1081 lcleeytlsg alerplhhse rvletvarwg ywdpedrkdn vlilkkdqly kdiaplvtpp
     1141 mttagelkfa dkksknfkny lfefshakly cykdkfcsnk lyewkiedii wyvghepkrn
     1201 pqtgwsitfi akntkptrck espffgntla gslkdeqykw laamtfaeyq lnirppsinl
     1261 mep
//
LOCUS       XP_034171362            1197 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase 10D [Osmia lignaria lignaria].
ACCESSION   XP_034171362
VERSION     XP_034171362.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315471.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171362.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1197
                     /product="tyrosine-protein phosphatase 10D"
                     /calculated_mol_wt=136748
     Region          78..145
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Region          172..240
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Region          260..324
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          460..560
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(460,533,548)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(549..550,552..553)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          913..1168
                     /region_name="PTPc"
                     /note="Protein tyrosine phosphatase, catalytic domain;
                     smart00194"
                     /db_xref="CDD:214550"
     CDS             1..1197
                     /gene="LOC117600276"
                     /coded_by="XM_034315471.2:404..3997"
                     /db_xref="GeneID:117600276"
ORIGIN      
        1 mivkkqllll iytlqiiyvl gedslidpke netnssrkiv sldridyfts pkvndtypkt
       61 enletesflc nttdhktvqn ltvdfvstew illswqppce npinesftyl iyvcsnekcf
      121 qtnekntryn asnldpctey eftvkvaqth peldgvnvta ktnyniteig dvqdllvhtt
      181 tstinlswra pskypecvqn yllticsnkt cdsqytihke yiakdlvpct kysviiravs
      241 yivksdglni tlktnspkss kprdptveas atslfihwqp pevgsmcith yrvtinpett
      301 ttistetnit innlractsy svyinpvdqd nndgdmvvin tktiptvsrp pilnnkesfv
      361 tmntimlswk iekgnsscil tslralcnyt staghgyeit ngqvevpins giqdeflfin
      421 slikevspft tyicwayvin eagiselskl tsfttfedip sspiatvrnv tysqfeiawe
      481 tpaylpgnlh efeivvewei cfpipdwctl kpsrnsihln gstfnfeyss aipytnytik
      541 mkarttagwg nysdnivfqt pagvpemvsn fsyliknneh dtntldtiln wgfpcsvngi
      601 ieyfnvfvng trtnyapdsf tnkqyipnti skndiisinl kelkaeynyt fevsvkvkgv
      661 qdfgeparht vlypagipsq pdedyiksit idpskaqrst tsatlllplf pdingdavyy
      721 siivsriern tasntrfnlt nhiwpnissw aeamlndfti pyqatrlwwd pypnyvadyg
      781 dmkavkytig edinckefss ntnkkvycng plkpntwyhv rmrafthggy adsttfvikt
      841 naeinvglvt giifgilffg isttvmllvr kyspqviirr flhpdmsgsp vpapfskkkf
      901 marcqqlidn pgklsnefql lqtlsvdlqm ptntaclqan kkknrysdil pydfsrvkle
      961 vidndpntdy inasfikgys gedeyiacqg pkeettfdfw rmieqynvni ivmlteliek
     1021 gkekcyqyfp iiketfkyen mtircisele yrsytqrtlv lqkenkkrni thlhfkewpd
     1081 hdvpegfdsm infcqivrrn vlankgyivv hcsagigrtg tliaidillq hirdnrkldv
     1141 fgtvyrlrrh rinmvqkesq yayiyncikq vlknpyclkt ykpppmdpfh qgtskkk
//
LOCUS       XP_034171370            1197 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase 10D [Osmia lignaria lignaria].
ACCESSION   XP_034171370
VERSION     XP_034171370.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315479.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171370.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1197
                     /product="tyrosine-protein phosphatase 10D"
                     /calculated_mol_wt=136748
     Region          78..145
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Region          172..240
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Region          260..324
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          460..560
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(460,533,548)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(549..550,552..553)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          913..1168
                     /region_name="PTPc"
                     /note="Protein tyrosine phosphatase, catalytic domain;
                     smart00194"
                     /db_xref="CDD:214550"
     CDS             1..1197
                     /gene="LOC117600276"
                     /coded_by="XM_034315479.2:338..3931"
                     /db_xref="GeneID:117600276"
ORIGIN      
        1 mivkkqllll iytlqiiyvl gedslidpke netnssrkiv sldridyfts pkvndtypkt
       61 enletesflc nttdhktvqn ltvdfvstew illswqppce npinesftyl iyvcsnekcf
      121 qtnekntryn asnldpctey eftvkvaqth peldgvnvta ktnyniteig dvqdllvhtt
      181 tstinlswra pskypecvqn yllticsnkt cdsqytihke yiakdlvpct kysviiravs
      241 yivksdglni tlktnspkss kprdptveas atslfihwqp pevgsmcith yrvtinpett
      301 ttistetnit innlractsy svyinpvdqd nndgdmvvin tktiptvsrp pilnnkesfv
      361 tmntimlswk iekgnsscil tslralcnyt staghgyeit ngqvevpins giqdeflfin
      421 slikevspft tyicwayvin eagiselskl tsfttfedip sspiatvrnv tysqfeiawe
      481 tpaylpgnlh efeivvewei cfpipdwctl kpsrnsihln gstfnfeyss aipytnytik
      541 mkarttagwg nysdnivfqt pagvpemvsn fsyliknneh dtntldtiln wgfpcsvngi
      601 ieyfnvfvng trtnyapdsf tnkqyipnti skndiisinl kelkaeynyt fevsvkvkgv
      661 qdfgeparht vlypagipsq pdedyiksit idpskaqrst tsatlllplf pdingdavyy
      721 siivsriern tasntrfnlt nhiwpnissw aeamlndfti pyqatrlwwd pypnyvadyg
      781 dmkavkytig edinckefss ntnkkvycng plkpntwyhv rmrafthggy adsttfvikt
      841 naeinvglvt giifgilffg isttvmllvr kyspqviirr flhpdmsgsp vpapfskkkf
      901 marcqqlidn pgklsnefql lqtlsvdlqm ptntaclqan kkknrysdil pydfsrvkle
      961 vidndpntdy inasfikgys gedeyiacqg pkeettfdfw rmieqynvni ivmlteliek
     1021 gkekcyqyfp iiketfkyen mtircisele yrsytqrtlv lqkenkkrni thlhfkewpd
     1081 hdvpegfdsm infcqivrrn vlankgyivv hcsagigrtg tliaidillq hirdnrkldv
     1141 fgtvyrlrrh rinmvqkesq yayiyncikq vlknpyclkt ykpppmdpfh qgtskkk
//
LOCUS       XP_034171380            1197 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase 10D [Osmia lignaria lignaria].
ACCESSION   XP_034171380
VERSION     XP_034171380.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171380.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1197
                     /product="tyrosine-protein phosphatase 10D"
                     /calculated_mol_wt=136748
     Region          78..145
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Region          172..240
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Region          260..324
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          460..560
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(460,533,548)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            order(549..550,552..553)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          913..1168
                     /region_name="PTPc"
                     /note="Protein tyrosine phosphatase, catalytic domain;
                     smart00194"
                     /db_xref="CDD:214550"
     CDS             1..1197
                     /gene="LOC117600276"
                     /coded_by="XM_034315489.2:636..4229"
                     /db_xref="GeneID:117600276"
ORIGIN      
        1 mivkkqllll iytlqiiyvl gedslidpke netnssrkiv sldridyfts pkvndtypkt
       61 enletesflc nttdhktvqn ltvdfvstew illswqppce npinesftyl iyvcsnekcf
      121 qtnekntryn asnldpctey eftvkvaqth peldgvnvta ktnyniteig dvqdllvhtt
      181 tstinlswra pskypecvqn yllticsnkt cdsqytihke yiakdlvpct kysviiravs
      241 yivksdglni tlktnspkss kprdptveas atslfihwqp pevgsmcith yrvtinpett
      301 ttistetnit innlractsy svyinpvdqd nndgdmvvin tktiptvsrp pilnnkesfv
      361 tmntimlswk iekgnsscil tslralcnyt staghgyeit ngqvevpins giqdeflfin
      421 slikevspft tyicwayvin eagiselskl tsfttfedip sspiatvrnv tysqfeiawe
      481 tpaylpgnlh efeivvewei cfpipdwctl kpsrnsihln gstfnfeyss aipytnytik
      541 mkarttagwg nysdnivfqt pagvpemvsn fsyliknneh dtntldtiln wgfpcsvngi
      601 ieyfnvfvng trtnyapdsf tnkqyipnti skndiisinl kelkaeynyt fevsvkvkgv
      661 qdfgeparht vlypagipsq pdedyiksit idpskaqrst tsatlllplf pdingdavyy
      721 siivsriern tasntrfnlt nhiwpnissw aeamlndfti pyqatrlwwd pypnyvadyg
      781 dmkavkytig edinckefss ntnkkvycng plkpntwyhv rmrafthggy adsttfvikt
      841 naeinvglvt giifgilffg isttvmllvr kyspqviirr flhpdmsgsp vpapfskkkf
      901 marcqqlidn pgklsnefql lqtlsvdlqm ptntaclqan kkknrysdil pydfsrvkle
      961 vidndpntdy inasfikgys gedeyiacqg pkeettfdfw rmieqynvni ivmlteliek
     1021 gkekcyqyfp iiketfkyen mtircisele yrsytqrtlv lqkenkkrni thlhfkewpd
     1081 hdvpegfdsm infcqivrrn vlankgyivv hcsagigrtg tliaidillq hirdnrkldv
     1141 fgtvyrlrrh rinmvqkesq yayiyncikq vlknpyclkt ykpppmdpfh qgtskkk
//
LOCUS       XP_034171396            1122 aa            linear   INV 03-SEP-2025
DEFINITION  protein broad-minded isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034171396
VERSION     XP_034171396.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171396.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1122
                     /product="protein broad-minded isoform X1"
                     /calculated_mol_wt=130350
     Region          1..1113
                     /region_name="RabGAP-TBC"
                     /note="Rab-GTPase-TBC domain; cl46302"
                     /db_xref="CDD:480642"
     CDS             1..1122
                     /gene="LOC117600286"
                     /coded_by="XM_034315505.2:138..3506"
                     /db_xref="GeneID:117600286"
ORIGIN      
        1 mennissnlq kwikkclrhe fetkvqksln ehvdytdiak klihskemst llnsmkatva
       61 eqsasrstst visdsrpqss fscisdqtce dwnsplnnde cyniiiykis qdkpahvrla
      121 gyeillksdl snlnsnpvwd llrkalldgl tdesrsifaa slqvhaklls clqshdvytn
      181 lldafnaqyh sqktfeilpt liaginfkff lhervfriih liiryneekl kttrnpdkst
      241 eelteqfitf lsthefgssm qpkplnilni isvlepcadw skkwminfit rkmflnalgk
      301 spsllqqimh ytqkgleepp hsitisiydd smeviingnt iktvtylhcl cfisqlcaye
      361 agrkileenv fdppfsisef ltaclkalnk lametlngvy dvscyalqii ldkptilyny
      421 efyhialchl sslsenniki wvhtlniilh mldtadgssf liseckehtv nfensvskcp
      481 amfivmiasn mlkqpfsiin ieyllklfkv meklfdifdv yeiiqhkiek efypavsyfy
      541 hklnksylen eikaqqmnsa vntlllkmvs iplglkvlvq etsvfqelie gsiaplrmsw
      601 tsmevvnfis saayfqlghd viatlaphvl snllletcki leapdyfydp wdhgiiqnfl
      661 hiltffslnf kcfsafmtnf kksdnneely ypvnllqlfq ssinsesnyh ylgllslntv
      721 iwnlniclyl lellnfqnil lelqnlnteg gneefscsci deyavmrqki isktyfiisk
      781 deeevfkset qdltklspwe snekdtsfse teydselenl lrenkpglld snwvqqirda
      841 ykvssdplkd tvliqlvdqm qkaiptadwg ehfqwqenit sstdfwfpee ihginlalhy
      901 aeqneilent veikeklqef inacyrfiny erpkkfdgfd wflstvfiic kgeiqrckif
      961 itqliqfpvt iflwpnlgkv ldkymreeca tqfkfmqvle sivsnefphi kfalkntsgm
     1021 nwslicnlmi sqcfwgllsw pqimnffaic ilyssdyiiy ycvsllyhcq hkimeditng
     1081 klwpehmvln eyqchdyikf mdtldkrygn rilpkftikg fn
//
LOCUS       XP_076546074            1075 aa            linear   INV 03-SEP-2025
DEFINITION  protein broad-minded isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546074
VERSION     XP_076546074.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689959.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1075
                     /product="protein broad-minded isoform X2"
                     /calculated_mol_wt=124712
     Region          1..1066
                     /region_name="RabGAP-TBC"
                     /note="Rab-GTPase-TBC domain; cl46302"
                     /db_xref="CDD:480642"
     CDS             1..1075
                     /gene="LOC117600286"
                     /coded_by="XM_076689959.1:248..3475"
                     /db_xref="GeneID:117600286"
ORIGIN      
        1 mstllnsmka tvaeqsasrs tstvisdsrp qssfscisdq tcedwnspln ndecyniiiy
       61 kisqdkpahv rlagyeillk sdlsnlnsnp vwdllrkall dgltdesrsi faaslqvhak
      121 llsclqshdv ytnlldafna qyhsqktfei lptliaginf kfflhervfr iihliiryne
      181 eklkttrnpd ksteelteqf itflsthefg ssmqpkplni lniisvlepc adwskkwmin
      241 fitrkmflna lgkspsllqq imhytqkgle epphsitisi yddsmeviin gntiktvtyl
      301 hclcfisqlc ayeagrkile envfdppfsi sefltaclka lnklametln gvydvscyal
      361 qiildkptil ynyefyhial chlsslsenn ikiwvhtlni ilhmldtadg ssflisecke
      421 htvnfensvs kcpamfivmi asnmlkqpfs iinieyllkl fkvmeklfdi fdvyeiiqhk
      481 iekefypavs yfyhklnksy leneikaqqm nsavntlllk mvsiplglkv lvqetsvfqe
      541 liegsiaplr mswtsmevvn fissaayfql ghdviatlap hvlsnlllet ckileapdyf
      601 ydpwdhgiiq nflhiltffs lnfkcfsafm tnfkksdnne elyypvnllq lfqssinses
      661 nyhylgllsl ntviwnlnic lyllellnfq nillelqnln teggneefsc scideyavmr
      721 qkiisktyfi iskdeeevfk setqdltkls pwesnekdts fseteydsel enllrenkpg
      781 lldsnwvqqi rdaykvssdp lkdtvliqlv dqmqkaipta dwgehfqwqe nitsstdfwf
      841 peeihginla lhyaeqneil entveikekl qefinacyrf inyerpkkfd gfdwflstvf
      901 iickgeiqrc kifitqliqf pvtiflwpnl gkvldkymre ecatqfkfmq vlesivsnef
      961 phikfalknt sgmnwslicn lmisqcfwgl lswpqimnff aicilyssdy iiyycvslly
     1021 hcqhkimedi tngklwpehm vlneyqchdy ikfmdtldkr ygnrilpkft ikgfn
//
LOCUS       XP_034171412             810 aa            linear   INV 03-SEP-2025
DEFINITION  TBC domain-containing protein kinase-like protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034171412
VERSION     XP_034171412.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315521.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171412.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..810
                     /product="TBC domain-containing protein kinase-like
                     protein"
                     /calculated_mol_wt=92553
     Region          47..257
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          426..630
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          695..799
                     /region_name="RHOD"
                     /note="Rhodanese Homology Domain (RHOD); an alpha beta
                     fold domain found duplicated in the rhodanese protein. The
                     cysteine containing enzymatically active version of the
                     domain is also found in the Cdc25 class of protein
                     phosphatases and a variety of proteins...; cl00125"
                     /db_xref="CDD:444705"
     Site            767
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238089"
     CDS             1..810
                     /gene="LOC117600288"
                     /coded_by="XM_034315521.2:163..2595"
                     /db_xref="GeneID:117600288"
ORIGIN      
        1 mcpallenee rcfggmtffa qshpvelcgs nglpltpnsi tiygksqflk tihhpylsty
       61 ldiirskher ivvvteyngd plsskenltv ddiikiafqc llglqhmntl glvhrhlspe
      121 nilintsgnv qlynfglyym tdsgknvsfp igypkytape iflssgvssp kvdswslgmi
      181 iaelligkli wpnvklsqcl rkvlslihce tsvferiare nncynvykel pdevkefids
      241 clqihpskrk tpeellklti fdnfllknek dkeenlyknv ivrkmdelyy lwqlaggdit
      301 gelkkqglir stppilsipn lvillgqmfg qrdtaslldl rivkipletl rqrlshipyi
      361 anypwltsqm hiqlqedlid aasqlpliir erdteyqfyr vvlydrllqv ypitreaiie
      421 eahkdipppv rgavwaalls itgdiqkryd midkdtptht drqievdipr chqysellss
      481 gagherlqrl lkawvrnnph yvywqgldsl tapflylnfn nearafecls afipkylhkf
      541 flkdnsaiiq eylgkfsqii afhdpqlanh lrsinfvpel faipwfltmf shvfplhkil
      601 hlwdklllgd ssfpllvgla ilkqlrdsll tsgfnecill fsdlpeidie lcvkdsmtmy
      661 qmtppsityr khqfnqpkdv nwsepepgte qmprisvddf mnlldndper lisvdirnnm
      721 qfergavlgs inipftsvql sqthietlgp hakplaeskn sivviigphd qnnalfadfl
      781 vkcgitgvcs lqggiyalrk kfpniimaar
//
LOCUS       XP_076546075             810 aa            linear   INV 03-SEP-2025
DEFINITION  TBC domain-containing protein kinase-like protein [Osmia lignaria
            lignaria].
ACCESSION   XP_076546075
VERSION     XP_076546075.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689960.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..810
                     /product="TBC domain-containing protein kinase-like
                     protein"
                     /calculated_mol_wt=92553
     Region          47..257
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          426..630
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          695..799
                     /region_name="RHOD"
                     /note="Rhodanese Homology Domain (RHOD); an alpha beta
                     fold domain found duplicated in the rhodanese protein. The
                     cysteine containing enzymatically active version of the
                     domain is also found in the Cdc25 class of protein
                     phosphatases and a variety of proteins...; cl00125"
                     /db_xref="CDD:444705"
     Site            767
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238089"
     CDS             1..810
                     /gene="LOC117600288"
                     /coded_by="XM_076689960.1:24..2456"
                     /db_xref="GeneID:117600288"
ORIGIN      
        1 mcpallenee rcfggmtffa qshpvelcgs nglpltpnsi tiygksqflk tihhpylsty
       61 ldiirskher ivvvteyngd plsskenltv ddiikiafqc llglqhmntl glvhrhlspe
      121 nilintsgnv qlynfglyym tdsgknvsfp igypkytape iflssgvssp kvdswslgmi
      181 iaelligkli wpnvklsqcl rkvlslihce tsvferiare nncynvykel pdevkefids
      241 clqihpskrk tpeellklti fdnfllknek dkeenlyknv ivrkmdelyy lwqlaggdit
      301 gelkkqglir stppilsipn lvillgqmfg qrdtaslldl rivkipletl rqrlshipyi
      361 anypwltsqm hiqlqedlid aasqlpliir erdteyqfyr vvlydrllqv ypitreaiie
      421 eahkdipppv rgavwaalls itgdiqkryd midkdtptht drqievdipr chqysellss
      481 gagherlqrl lkawvrnnph yvywqgldsl tapflylnfn nearafecls afipkylhkf
      541 flkdnsaiiq eylgkfsqii afhdpqlanh lrsinfvpel faipwfltmf shvfplhkil
      601 hlwdklllgd ssfpllvgla ilkqlrdsll tsgfnecill fsdlpeidie lcvkdsmtmy
      661 qmtppsityr khqfnqpkdv nwsepepgte qmprisvddf mnlldndper lisvdirnnm
      721 qfergavlgs inipftsvql sqthietlgp hakplaeskn sivviigphd qnnalfadfl
      781 vkcgitgvcs lqggiyalrk kfpniimaar
//
LOCUS       XP_034171428             663 aa            linear   INV 03-SEP-2025
DEFINITION  apoptosis inducing factor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034171428
VERSION     XP_034171428.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315537.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171428.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..663
                     /product="apoptosis inducing factor isoform X1"
                     /calculated_mol_wt=73866
     Region          173..647
                     /region_name="NirB"
                     /note="NAD(P)H-nitrite reductase, large subunit [Energy
                     production and conversion]; COG1251"
                     /db_xref="CDD:440863"
     CDS             1..663
                     /gene="AIF"
                     /coded_by="XM_034315537.2:195..2186"
                     /db_xref="GeneID:117600290"
ORIGIN      
        1 mlscgrvigq lpkltrhsha nrfcrplkyi givsinnswf ssnvsdkkty kppgttikpe
       61 ecvpdstylk pkrniplcel spnascpssc yevcgksdds qsskpppppp ywkilatlli
      121 agvtiyaiss snwftskksd sqkdvdkkds rrhrrtdkik spptsssfpq hvpylliggg
      181 taafsafrsi ksrdprakvl viteegdlpy mrpplskelw yntdrktsaq llfkqwngaq
      241 rsifyeppef yskikhlies ekggvavatg wkvtkidvsn kivtlddgye ikydkcliat
      301 gaspknlpvf eslpdevkdk irtfrtkeqf ldlednvhnp kcknivivgg gflgtelacs
      361 larnlpedkk vhqvykekfm mdqvlpqyls ewatrkatle gvncipnsev edyyykngql
      421 slilsgnnvi dadevivavg vepntdlakv shletdpeig gflvnaelea rsnlwvagda
      481 acfydirlgr rrvehhdhav vsgrlagenm tgagkpylhq smfwsdlgpe vgfeaigivd
      541 stlptvgvfv kaektdtsit aidtsnetak ssnekklhst qsqssnendk svrnvdskst
      601 aeeptnyddf ekgvifylrd divvgivlwn ifnrmsiarq vlakgtkydd lnevaklfti
      661 hdd
//
LOCUS       XP_034171438             636 aa            linear   INV 03-SEP-2025
DEFINITION  apoptosis inducing factor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034171438
VERSION     XP_034171438.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315547.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171438.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="apoptosis inducing factor isoform X2"
                     /calculated_mol_wt=70897
     Region          146..620
                     /region_name="NirB"
                     /note="NAD(P)H-nitrite reductase, large subunit [Energy
                     production and conversion]; COG1251"
                     /db_xref="CDD:440863"
     CDS             1..636
                     /gene="AIF"
                     /coded_by="XM_034315547.2:195..2105"
                     /db_xref="GeneID:117600290"
ORIGIN      
        1 mlscgrvigq lpkltrhsha nrfcrplkyi givsinnswf ssnvsdkkty kppgttikpe
       61 ecvpdicgks ddsqsskppp pppywkilat lliagvtiya isssnwftsk ksdsqkdvdk
      121 kdsrrhrrtd kikspptsss fpqhvpylli gggtaafsaf rsiksrdpra kvlviteegd
      181 lpymrpplsk elwyntdrkt saqllfkqwn gaqrsifyep pefyskikhl iesekggvav
      241 atgwkvtkid vsnkivtldd gyeikydkcl iatgaspknl pvfeslpdev kdkirtfrtk
      301 eqfldlednv hnpkcknivi vgggflgtel acslarnlpe dkkvhqvyke kfmmdqvlpq
      361 ylsewatrka tlegvncipn sevedyyykn gqlslilsgn nvidadeviv avgvepntdl
      421 akvshletdp eiggflvnae learsnlwva gdaacfydir lgrrrvehhd havvsgrlag
      481 enmtgagkpy lhqsmfwsdl gpevgfeaig ivdstlptvg vfvkaektdt sitaidtsne
      541 takssnekkl hstqsqssne ndksvrnvds kstaeeptny ddfekgvify lrddivvgiv
      601 lwnifnrmsi arqvlakgtk yddlnevakl ftihdd
//
LOCUS       XP_034171452             556 aa            linear   INV 03-SEP-2025
DEFINITION  SPARC related modular calcium binding-like protein magu isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034171452
VERSION     XP_034171452.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315561.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..556
                     /product="SPARC related modular calcium binding-like
                     protein magu isoform X1"
                     /calculated_mol_wt=63474
     Region          39..82
                     /region_name="KAZAL"
                     /note="Kazal type serine protease inhibitors; smart00280"
                     /db_xref="CDD:197624"
     Region          <101..143
                     /region_name="TY"
                     /note="Thyroglobulin type I repeats.; The N-terminal
                     region of human thyroglobulin contains 11 type-1 repeats
                     TY repeats are proposed to be inhibitors of cysteine
                     proteases; cd00191"
                     /db_xref="CDD:238114"
     Region          179..278
                     /region_name="EFh_SPARC_SMOC"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted modular calcium-binding protein SMOC-1,
                     SMOC-2, and similar proteins; cd16234"
                     /db_xref="CDD:320013"
     Region          212..244
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     Site            order(224,226,228,235,261,263,265,272)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320013"
     Region          249..278
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     Region          340..403
                     /region_name="Thyroglobulin_1"
                     /note="Thyroglobulin type-1 repeat; pfam00086"
                     /db_xref="CDD:459665"
     Site            order(344,359,377,379)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     Region          416..521
                     /region_name="EFh_SPARC_SMOC"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted modular calcium-binding protein SMOC-1,
                     SMOC-2, and similar proteins; cd16234"
                     /db_xref="CDD:320013"
     Region          455..487
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     Site            order(467,469,471,478,504,506,508,515)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320013"
     Region          492..521
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     CDS             1..556
                     /gene="magu"
                     /coded_by="XM_034315561.2:4130..5800"
                     /db_xref="GeneID:117600296"
ORIGIN      
        1 mmmipmirta illyvfvhld atantittak eecrkriaec tmsdgastpv cgsdgvtyss
       61 qcqviskqcq gmsilikhtg pcpetpacfs arltarpgar pvcrpdgtya pvqcheetgy
      121 cwcvtpqgrp lpdtsvrhqr prclkpgprs aastrsgqrr rspnwkqrrq ysskhrntcd
      181 raekskfngn lienfkieyr rtnisadgdk nvervlswkf vtldkngdgy ldraeykelr
      241 rlakkavrpk kcartfartc dlnrdlklsr qewgacland ftlrlflsln paderpevpe
      301 aqrtratdqv lkgnpapvhs rpafnddpmd tkedsdandc ltdrrsvled erqhsqekfy
      361 vpectpdgry hrvqcysgyc wcvyqdtgkp ipgtsskdrt pncnpvptps rpmkgcpeqk
      421 kqlflrdlmd lmqkkmkasg tdsdettakw qaskeeqiat whfvmldknk nkvlerkewk
      481 sfrtmvannr qlqrcgkrlp rycdinndrr ismtewlscl naqrpttses tekasttkpk
      541 rmgpnpldqf lknddd
//
LOCUS       XP_034171460             546 aa            linear   INV 03-SEP-2025
DEFINITION  SPARC related modular calcium binding-like protein magu isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034171460
VERSION     XP_034171460.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315569.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="SPARC related modular calcium binding-like
                     protein magu isoform X2"
                     /calculated_mol_wt=62371
     Region          39..82
                     /region_name="KAZAL"
                     /note="Kazal type serine protease inhibitors; smart00280"
                     /db_xref="CDD:197624"
     Region          <101..143
                     /region_name="TY"
                     /note="Thyroglobulin type I repeats.; The N-terminal
                     region of human thyroglobulin contains 11 type-1 repeats
                     TY repeats are proposed to be inhibitors of cysteine
                     proteases; cd00191"
                     /db_xref="CDD:238114"
     Region          179..278
                     /region_name="EFh_SPARC_SMOC"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted modular calcium-binding protein SMOC-1,
                     SMOC-2, and similar proteins; cd16234"
                     /db_xref="CDD:320013"
     Region          212..244
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     Site            order(224,226,228,235,261,263,265,272)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320013"
     Region          249..278
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     Region          330..393
                     /region_name="Thyroglobulin_1"
                     /note="Thyroglobulin type-1 repeat; pfam00086"
                     /db_xref="CDD:459665"
     Site            order(334,349,367,369)
                     /site_type="active"
                     /note="protease interaction site [active]"
                     /db_xref="CDD:238114"
     Region          406..511
                     /region_name="EFh_SPARC_SMOC"
                     /note="EF-hand, extracellular calcium-binding (EC) motif,
                     found in secreted modular calcium-binding protein SMOC-1,
                     SMOC-2, and similar proteins; cd16234"
                     /db_xref="CDD:320013"
     Region          445..477
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     Site            order(457,459,461,468,494,496,498,505)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:320013"
     Region          482..511
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320013"
     CDS             1..546
                     /gene="magu"
                     /coded_by="XM_034315569.2:4130..5770"
                     /db_xref="GeneID:117600296"
ORIGIN      
        1 mmmipmirta illyvfvhld atantittak eecrkriaec tmsdgastpv cgsdgvtyss
       61 qcqviskqcq gmsilikhtg pcpetpacfs arltarpgar pvcrpdgtya pvqcheetgy
      121 cwcvtpqgrp lpdtsvrhqr prclkpgprs aastrsgqrr rspnwkqrrq ysskhrntcd
      181 raekskfngn lienfkieyr rtnisadgdk nvervlswkf vtldkngdgy ldraeykelr
      241 rlakkavrpk kcartfartc dlnrdlklsr qewgacland ftherpevpe aqrtratdqv
      301 lkgnpapvhs rpafnddpmd tkedsdandc ltdrrsvled erqhsqekfy vpectpdgry
      361 hrvqcysgyc wcvyqdtgkp ipgtsskdrt pncnpvptps rpmkgcpeqk kqlflrdlmd
      421 lmqkkmkasg tdsdettakw qaskeeqiat whfvmldknk nkvlerkewk sfrtmvannr
      481 qlqrcgkrlp rycdinndrr ismtewlscl naqrpttses tekasttkpk rmgpnpldqf
      541 lknddd
//
LOCUS       XP_076546076             515 aa            linear   INV 03-SEP-2025
DEFINITION  T-complex protein 11-like protein 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546076
VERSION     XP_076546076.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689961.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="T-complex protein 11-like protein 1 isoform X1"
                     /calculated_mol_wt=58135
     Region          82..492
                     /region_name="Tcp11"
                     /note="T-complex protein 11; pfam05794"
                     /db_xref="CDD:461743"
     CDS             1..515
                     /gene="LOC117600309"
                     /coded_by="XM_076689961.1:584..2131"
                     /db_xref="GeneID:117600309"
ORIGIN      
        1 mivyrnqkmd ndeeqkcnin epgtsapldc qsnsnpdiee entkgqkisn fmigglvgas
       61 ppkfvtleei ikaangmknm alaheiavdk nfqlqklepd dgtfhrkvke imhkafwnll
      121 aeqlaedppn ytqalvllqe iketldelvl phhakirenl kevldidlik qqaekgvldf
      181 hhyaqyvisi mskvcapvrd ekirelgqkt dvieifrgim dvlqlmrldl anftitmmrp
      241 nivassieye kakfaeflki ngnglqytek wilshfdptk vtgsssdina vrqlthsllt
      301 eayldllewd fnpdaetlml dqgrllelrd ktsrlsiigs aillvnntvg spvhgvsslk
      361 neikqhlnil ldsvhsnkdl qtvmpnvllq iktdlkttlq eigaeplspe metllegqil
      421 dlinpdhkir hlislrirqf lqkiilsqsa apqhvppgls slqeeltaia aqflilishn
      481 rsvfgeyyqe ivtnalhkke meenkdtsgv htmel
//
LOCUS       XP_076546077             515 aa            linear   INV 03-SEP-2025
DEFINITION  T-complex protein 11-like protein 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546077
VERSION     XP_076546077.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689962.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="T-complex protein 11-like protein 1 isoform X1"
                     /calculated_mol_wt=58135
     Region          82..492
                     /region_name="Tcp11"
                     /note="T-complex protein 11; pfam05794"
                     /db_xref="CDD:461743"
     CDS             1..515
                     /gene="LOC117600309"
                     /coded_by="XM_076689962.1:610..2157"
                     /db_xref="GeneID:117600309"
ORIGIN      
        1 mivyrnqkmd ndeeqkcnin epgtsapldc qsnsnpdiee entkgqkisn fmigglvgas
       61 ppkfvtleei ikaangmknm alaheiavdk nfqlqklepd dgtfhrkvke imhkafwnll
      121 aeqlaedppn ytqalvllqe iketldelvl phhakirenl kevldidlik qqaekgvldf
      181 hhyaqyvisi mskvcapvrd ekirelgqkt dvieifrgim dvlqlmrldl anftitmmrp
      241 nivassieye kakfaeflki ngnglqytek wilshfdptk vtgsssdina vrqlthsllt
      301 eayldllewd fnpdaetlml dqgrllelrd ktsrlsiigs aillvnntvg spvhgvsslk
      361 neikqhlnil ldsvhsnkdl qtvmpnvllq iktdlkttlq eigaeplspe metllegqil
      421 dlinpdhkir hlislrirqf lqkiilsqsa apqhvppgls slqeeltaia aqflilishn
      481 rsvfgeyyqe ivtnalhkke meenkdtsgv htmel
//
LOCUS       XP_076546078             515 aa            linear   INV 03-SEP-2025
DEFINITION  T-complex protein 11-like protein 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546078
VERSION     XP_076546078.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689963.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="T-complex protein 11-like protein 1 isoform X1"
                     /calculated_mol_wt=58135
     Region          82..492
                     /region_name="Tcp11"
                     /note="T-complex protein 11; pfam05794"
                     /db_xref="CDD:461743"
     CDS             1..515
                     /gene="LOC117600309"
                     /coded_by="XM_076689963.1:270..1817"
                     /db_xref="GeneID:117600309"
ORIGIN      
        1 mivyrnqkmd ndeeqkcnin epgtsapldc qsnsnpdiee entkgqkisn fmigglvgas
       61 ppkfvtleei ikaangmknm alaheiavdk nfqlqklepd dgtfhrkvke imhkafwnll
      121 aeqlaedppn ytqalvllqe iketldelvl phhakirenl kevldidlik qqaekgvldf
      181 hhyaqyvisi mskvcapvrd ekirelgqkt dvieifrgim dvlqlmrldl anftitmmrp
      241 nivassieye kakfaeflki ngnglqytek wilshfdptk vtgsssdina vrqlthsllt
      301 eayldllewd fnpdaetlml dqgrllelrd ktsrlsiigs aillvnntvg spvhgvsslk
      361 neikqhlnil ldsvhsnkdl qtvmpnvllq iktdlkttlq eigaeplspe metllegqil
      421 dlinpdhkir hlislrirqf lqkiilsqsa apqhvppgls slqeeltaia aqflilishn
      481 rsvfgeyyqe ivtnalhkke meenkdtsgv htmel
//
LOCUS       XP_034171485             514 aa            linear   INV 03-SEP-2025
DEFINITION  T-complex protein 11-like protein 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171485
VERSION     XP_034171485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="T-complex protein 11-like protein 1 isoform X2"
                     /calculated_mol_wt=57972
     Region          81..491
                     /region_name="Tcp11"
                     /note="T-complex protein 11; pfam05794"
                     /db_xref="CDD:461743"
     CDS             1..514
                     /gene="LOC117600309"
                     /coded_by="XM_034315594.2:526..2070"
                     /db_xref="GeneID:117600309"
ORIGIN      
        1 mpdrnqkmdn deeqkcnine pgtsapldcq snsnpdieee ntkgqkisnf migglvgasp
       61 pkfvtleeii kaangmknma laheiavdkn fqlqklepdd gtfhrkvkei mhkafwnlla
      121 eqlaedppny tqalvllqei ketldelvlp hhakirenlk evldidlikq qaekgvldfh
      181 hyaqyvisim skvcapvrde kirelgqktd vieifrgimd vlqlmrldla nftitmmrpn
      241 ivassieyek akfaeflkin gnglqytekw ilshfdptkv tgsssdinav rqlthsllte
      301 ayldllewdf npdaetlmld qgrllelrdk tsrlsiigsa illvnntvgs pvhgvsslkn
      361 eikqhlnill dsvhsnkdlq tvmpnvllqi ktdlkttlqe igaeplspem etllegqild
      421 linpdhkirh lislrirqfl qkiilsqsaa pqhvppglss lqeeltaiaa qflilishnr
      481 svfgeyyqei vtnalhkkem eenkdtsgvh tmel
//
LOCUS       XP_034171494             514 aa            linear   INV 03-SEP-2025
DEFINITION  T-complex protein 11-like protein 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171494
VERSION     XP_034171494.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315603.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="T-complex protein 11-like protein 1 isoform X2"
                     /calculated_mol_wt=57972
     Region          81..491
                     /region_name="Tcp11"
                     /note="T-complex protein 11; pfam05794"
                     /db_xref="CDD:461743"
     CDS             1..514
                     /gene="LOC117600309"
                     /coded_by="XM_034315603.2:552..2096"
                     /db_xref="GeneID:117600309"
ORIGIN      
        1 mpdrnqkmdn deeqkcnine pgtsapldcq snsnpdieee ntkgqkisnf migglvgasp
       61 pkfvtleeii kaangmknma laheiavdkn fqlqklepdd gtfhrkvkei mhkafwnlla
      121 eqlaedppny tqalvllqei ketldelvlp hhakirenlk evldidlikq qaekgvldfh
      181 hyaqyvisim skvcapvrde kirelgqktd vieifrgimd vlqlmrldla nftitmmrpn
      241 ivassieyek akfaeflkin gnglqytekw ilshfdptkv tgsssdinav rqlthsllte
      301 ayldllewdf npdaetlmld qgrllelrdk tsrlsiigsa illvnntvgs pvhgvsslkn
      361 eikqhlnill dsvhsnkdlq tvmpnvllqi ktdlkttlqe igaeplspem etllegqild
      421 linpdhkirh lislrirqfl qkiilsqsaa pqhvppglss lqeeltaiaa qflilishnr
      481 svfgeyyqei vtnalhkkem eenkdtsgvh tmel
//
LOCUS       XP_034171502             514 aa            linear   INV 03-SEP-2025
DEFINITION  T-complex protein 11-like protein 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171502
VERSION     XP_034171502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="T-complex protein 11-like protein 1 isoform X2"
                     /calculated_mol_wt=57972
     Region          81..491
                     /region_name="Tcp11"
                     /note="T-complex protein 11; pfam05794"
                     /db_xref="CDD:461743"
     CDS             1..514
                     /gene="LOC117600309"
                     /coded_by="XM_034315611.2:211..1755"
                     /db_xref="GeneID:117600309"
ORIGIN      
        1 mpdrnqkmdn deeqkcnine pgtsapldcq snsnpdieee ntkgqkisnf migglvgasp
       61 pkfvtleeii kaangmknma laheiavdkn fqlqklepdd gtfhrkvkei mhkafwnlla
      121 eqlaedppny tqalvllqei ketldelvlp hhakirenlk evldidlikq qaekgvldfh
      181 hyaqyvisim skvcapvrde kirelgqktd vieifrgimd vlqlmrldla nftitmmrpn
      241 ivassieyek akfaeflkin gnglqytekw ilshfdptkv tgsssdinav rqlthsllte
      301 ayldllewdf npdaetlmld qgrllelrdk tsrlsiigsa illvnntvgs pvhgvsslkn
      361 eikqhlnill dsvhsnkdlq tvmpnvllqi ktdlkttlqe igaeplspem etllegqild
      421 linpdhkirh lislrirqfl qkiilsqsaa pqhvppglss lqeeltaiaa qflilishnr
      481 svfgeyyqei vtnalhkkem eenkdtsgvh tmel
//
LOCUS       XP_034171524             464 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600322 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171524
VERSION     XP_034171524.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315633.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171524.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="uncharacterized protein LOC117600322 isoform X1"
                     /calculated_mol_wt=51311
     Region          312..332
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          372..392
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          400..421
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..464
                     /gene="LOC117600322"
                     /coded_by="XM_034315633.2:145..1539"
                     /db_xref="GeneID:117600322"
ORIGIN      
        1 meygiwslha agelwadprl arrhsilpas atqllpraaf alsglhrpdm lplpltstpd
       61 pmepygpmel vakpsadrda ekseaeeekh ednegniekp dendyqtsvp kenndnedag
      121 eesdtgstns shrsannnls tklldpmayl tfdkegavtp vlngwvlsay tamlgrlsaa
      181 taaleateip nvpsdsdses ehssyteksr gsspnvsnah rgyscgscdv vaptrpalrk
      241 hivdchppvs stetgetksc pssgcdftts srcemethva ahvaqgmtpt gkkrtlalqr
      301 vryryereey rcslcsyact iekafqrhlr ahakgtapet rvscavcgad rssevdlsrh
      361 mrrhrddryf ccdicifrtv qlkkliqhrr mhtgekphlc phcayrsarr dnlrshvrrv
      421 hkkenlycdt fsprgmlltp tigrdspvvq spesspssns esan
//
LOCUS       XP_034171541             464 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600322 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171541
VERSION     XP_034171541.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315650.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171541.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="uncharacterized protein LOC117600322 isoform X1"
                     /calculated_mol_wt=51311
     Region          312..332
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          372..392
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          400..421
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..464
                     /gene="LOC117600322"
                     /coded_by="XM_034315650.2:137..1531"
                     /db_xref="GeneID:117600322"
ORIGIN      
        1 meygiwslha agelwadprl arrhsilpas atqllpraaf alsglhrpdm lplpltstpd
       61 pmepygpmel vakpsadrda ekseaeeekh ednegniekp dendyqtsvp kenndnedag
      121 eesdtgstns shrsannnls tklldpmayl tfdkegavtp vlngwvlsay tamlgrlsaa
      181 taaleateip nvpsdsdses ehssyteksr gsspnvsnah rgyscgscdv vaptrpalrk
      241 hivdchppvs stetgetksc pssgcdftts srcemethva ahvaqgmtpt gkkrtlalqr
      301 vryryereey rcslcsyact iekafqrhlr ahakgtapet rvscavcgad rssevdlsrh
      361 mrrhrddryf ccdicifrtv qlkkliqhrr mhtgekphlc phcayrsarr dnlrshvrrv
      421 hkkenlycdt fsprgmlltp tigrdspvvq spesspssns esan
//
LOCUS       XP_034171515             464 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600322 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171515
VERSION     XP_034171515.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315624.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171515.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="uncharacterized protein LOC117600322 isoform X1"
                     /calculated_mol_wt=51311
     Region          312..332
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          372..392
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          400..421
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..464
                     /gene="LOC117600322"
                     /coded_by="XM_034315624.2:1776..3170"
                     /db_xref="GeneID:117600322"
ORIGIN      
        1 meygiwslha agelwadprl arrhsilpas atqllpraaf alsglhrpdm lplpltstpd
       61 pmepygpmel vakpsadrda ekseaeeekh ednegniekp dendyqtsvp kenndnedag
      121 eesdtgstns shrsannnls tklldpmayl tfdkegavtp vlngwvlsay tamlgrlsaa
      181 taaleateip nvpsdsdses ehssyteksr gsspnvsnah rgyscgscdv vaptrpalrk
      241 hivdchppvs stetgetksc pssgcdftts srcemethva ahvaqgmtpt gkkrtlalqr
      301 vryryereey rcslcsyact iekafqrhlr ahakgtapet rvscavcgad rssevdlsrh
      361 mrrhrddryf ccdicifrtv qlkkliqhrr mhtgekphlc phcayrsarr dnlrshvrrv
      421 hkkenlycdt fsprgmlltp tigrdspvvq spesspssns esan
//
LOCUS       XP_034171551             462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600322 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171551
VERSION     XP_034171551.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171551.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="uncharacterized protein LOC117600322 isoform X2"
                     /calculated_mol_wt=50992
     Region          310..330
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          370..390
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          398..419
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..462
                     /gene="LOC117600322"
                     /coded_by="XM_034315660.2:157..1545"
                     /db_xref="GeneID:117600322"
ORIGIN      
        1 meygiwslha agelwadprl arrhsilpas atqllpraaf alsglhrpdm lplpltstpd
       61 pmepygpmel vakpsadrda ekseaeeekh ednegniekp dendyqtsvp kenndnedag
      121 eesdtgstns shrsannnls tklldpmayl tfdkegavtp vlngwvlsay tamlgrlsaa
      181 taaleateip nvpsdsdses ehssyteksr gsspnvsnah rgyscgscdv vaptrpalrk
      241 hivdchppvs stetgetksc pssgcdftts srcemethva ahvaqgmtpt gkkrtlalqr
      301 vryereeyrc slcsyactie kafqrhlrah akgtapetrv scavcgadrs sevdlsrhmr
      361 rhrddryfcc dicifrtvql kkliqhrrmh tgekphlcph cayrsarrdn lrshvrrvhk
      421 kenlycdtfs prgmlltpti grdspvvqsp esspssnses an
//
LOCUS       XP_034171565             382 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin-dependent kinase 9 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034171565
VERSION     XP_034171565.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315674.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..382
                     /product="cyclin-dependent kinase 9 isoform X1"
                     /calculated_mol_wt=43912
     Region          11..321
                     /region_name="STKc_CDK9"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Cyclin-Dependent protein Kinase 9; cd07865"
                     /db_xref="CDD:270848"
     Site            order(13..18,63..65,67,70,74,77,87..90,92,105)
                     /site_type="other"
                     /note="CDK/cyclin interface [polypeptide binding]"
                     /db_xref="CDD:270848"
     Site            order(30..33,38,52,54,71,85,109..112,115,117..118,155,157,
                     159..160,162,173,176,192,194..197,199,237..238)
                     /site_type="active"
                     /db_xref="CDD:270848"
     Site            order(30..33,38,52,54,85,109..112,115,118,159..160,162,
                     173)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270848"
     Site            order(71,117,155,157,176,192,194..197,199,237..238)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270848"
     Site            order(172..186,189..199)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270848"
     CDS             1..382
                     /gene="Cdk9"
                     /coded_by="XM_034315674.2:79..1227"
                     /db_xref="GeneID:117600342"
ORIGIN      
        1 mntrekekyi eefefphcde sskyekvaki gqgtfgevfk ardknctkkf vamkkvlmdn
       61 ekegfpital reirilqllk henvvnliei crtratqynr yrstfylvfd fcehdlagll
      121 snvnvkfslg eikkvmqqll nglyyihsnk ilhrdmkaan vlitkngilk ladfglaraf
      181 sankngqpnr ytnrvvtlwy rppelllgdr nygppvdlwg agcimaemwt rspimqgnte
      241 qqqlilisql cgsittevwp gvenlelfnk mdlpkgqkrk vkdrlkpylk dpyacdlldk
      301 llildpskry dsdsalnhdf fwtdpmpcdl skmlaqhtqs mfeylapprr pghirhphhq
      361 vpggsakpss smadsgyqdr vf
//
LOCUS       XP_034171574             276 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin-dependent kinase 9 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034171574
VERSION     XP_034171574.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315683.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="cyclin-dependent kinase 9 isoform X2"
                     /calculated_mol_wt=31691
     Region          11..276
                     /region_name="STKc_CDK9"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Cyclin-Dependent protein Kinase 9; cd07865"
                     /db_xref="CDD:270848"
     Site            order(13..18,63..65,67,70,74,77,87..90,92,105)
                     /site_type="other"
                     /note="CDK/cyclin interface [polypeptide binding]"
                     /db_xref="CDD:270848"
     Site            order(30..33,38,52,54,71,85,109..112,115,117..118,155,157,
                     159..160,162,173,176,192,194..197,199,237..238)
                     /site_type="active"
                     /db_xref="CDD:270848"
     Site            order(30..33,38,52,54,85,109..112,115,118,159..160,162,
                     173)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270848"
     Site            order(71,117,155,157,176,192,194..197,199,237..238)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270848"
     Site            order(172..186,189..199)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270848"
     CDS             1..276
                     /gene="Cdk9"
                     /coded_by="XM_034315683.2:79..909"
                     /db_xref="GeneID:117600342"
ORIGIN      
        1 mntrekekyi eefefphcde sskyekvaki gqgtfgevfk ardknctkkf vamkkvlmdn
       61 ekegfpital reirilqllk henvvnliei crtratqynr yrstfylvfd fcehdlagll
      121 snvnvkfslg eikkvmqqll nglyyihsnk ilhrdmkaan vlitkngilk ladfglaraf
      181 sankngqpnr ytnrvvtlwy rppelllgdr nygppvdlwg agcimaemwt rspimqgnte
      241 qqqlilisql cgsittevwp gvenlelfnk mdlpkg
//
LOCUS       XP_034171597             333 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-galactose transporter [Osmia lignaria lignaria].
ACCESSION   XP_034171597
VERSION     XP_034171597.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315706.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171597.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="UDP-galactose transporter"
                     /calculated_mol_wt=36648
     Region          12..316
                     /region_name="Nuc_sug_transp"
                     /note="Nucleotide-sugar transporter; pfam04142"
                     /db_xref="CDD:398009"
     CDS             1..333
                     /gene="Ugalt"
                     /coded_by="XM_034315706.2:297..1298"
                     /db_xref="GeneID:117600360"
ORIGIN      
        1 mnkpqqrnnq alkyislitl tlqnaivgls mryartrsgd mflsstavvm aevvklitcl
       61 ilvlleegnl qkffysless iikqpidtlk vcvpsllyii qnnllyvsas nldaatyqvt
      121 yqlkilttaf favvilkrsl rntqwgalvl liigvvlvql aqssgttlps gieqnhligf
      181 saalsacfls gfagiyfeki lkdsnksvwm rnvqlsllsl pfglmtcfin dgatlkkqgf
      241 ffgydlfvyy lvvlqagggl ivamvvkyad nilkgfatsl aiiisciasi ylfdfkltlq
      301 fslgavlvic sifmygyqpk stftdthspt nkv
//
LOCUS       XP_076546079             333 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-galactose transporter [Osmia lignaria lignaria].
ACCESSION   XP_076546079
VERSION     XP_076546079.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689964.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="UDP-galactose transporter"
                     /calculated_mol_wt=36648
     Region          12..316
                     /region_name="Nuc_sug_transp"
                     /note="Nucleotide-sugar transporter; pfam04142"
                     /db_xref="CDD:398009"
     CDS             1..333
                     /gene="Ugalt"
                     /coded_by="XM_076689964.1:122..1123"
                     /db_xref="GeneID:117600360"
ORIGIN      
        1 mnkpqqrnnq alkyislitl tlqnaivgls mryartrsgd mflsstavvm aevvklitcl
       61 ilvlleegnl qkffysless iikqpidtlk vcvpsllyii qnnllyvsas nldaatyqvt
      121 yqlkilttaf favvilkrsl rntqwgalvl liigvvlvql aqssgttlps gieqnhligf
      181 saalsacfls gfagiyfeki lkdsnksvwm rnvqlsllsl pfglmtcfin dgatlkkqgf
      241 ffgydlfvyy lvvlqagggl ivamvvkyad nilkgfatsl aiiisciasi ylfdfkltlq
      301 fslgavlvic sifmygyqpk stftdthspt nkv
//
LOCUS       XP_034171610             283 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600366 [Osmia lignaria lignaria].
ACCESSION   XP_034171610
VERSION     XP_034171610.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315719.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="uncharacterized protein LOC117600366"
                     /calculated_mol_wt=32601
     CDS             1..283
                     /gene="LOC117600366"
                     /coded_by="XM_034315719.2:331..1182"
                     /db_xref="GeneID:117600366"
ORIGIN      
        1 mqkeqpepsq thrikrtrna stpdrikkni mdstmtnirf qhratmsaad falpqssskr
       61 kltmdrsvmg psqilsegns vidsesndve lkllydeylq ntmteiilkk kaeerkqlfl
      121 sqlatiakey ehneeklfel ktrerdiinl tkiqndidkq itdinnciks edskklndil
      181 sklysllqpl dklrcnniil petpeeweem mknfkscnat lesimdligt kkesyqnvns
      241 gikefintfn nienytkrle kdisdleali lksaslslaq sdn
//
LOCUS       XP_034171620             283 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600366 [Osmia lignaria lignaria].
ACCESSION   XP_034171620
VERSION     XP_034171620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="uncharacterized protein LOC117600366"
                     /calculated_mol_wt=32601
     CDS             1..283
                     /gene="LOC117600366"
                     /coded_by="XM_034315729.2:331..1182"
                     /db_xref="GeneID:117600366"
ORIGIN      
        1 mqkeqpepsq thrikrtrna stpdrikkni mdstmtnirf qhratmsaad falpqssskr
       61 kltmdrsvmg psqilsegns vidsesndve lkllydeylq ntmteiilkk kaeerkqlfl
      121 sqlatiakey ehneeklfel ktrerdiinl tkiqndidkq itdinnciks edskklndil
      181 sklysllqpl dklrcnniil petpeeweem mknfkscnat lesimdligt kkesyqnvns
      241 gikefintfn nienytkrle kdisdleali lksaslslaq sdn
//
LOCUS       XP_034171627             283 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600366 [Osmia lignaria lignaria].
ACCESSION   XP_034171627
VERSION     XP_034171627.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315736.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="uncharacterized protein LOC117600366"
                     /calculated_mol_wt=32601
     CDS             1..283
                     /gene="LOC117600366"
                     /coded_by="XM_034315736.2:144..995"
                     /db_xref="GeneID:117600366"
ORIGIN      
        1 mqkeqpepsq thrikrtrna stpdrikkni mdstmtnirf qhratmsaad falpqssskr
       61 kltmdrsvmg psqilsegns vidsesndve lkllydeylq ntmteiilkk kaeerkqlfl
      121 sqlatiakey ehneeklfel ktrerdiinl tkiqndidkq itdinnciks edskklndil
      181 sklysllqpl dklrcnniil petpeeweem mknfkscnat lesimdligt kkesyqnvns
      241 gikefintfn nienytkrle kdisdleali lksaslslaq sdn
//
LOCUS       XP_076546080             264 aa            linear   INV 03-SEP-2025
DEFINITION  pyrimidodiazepine synthase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546080
VERSION     XP_076546080.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689965.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..264
                     /product="pyrimidodiazepine synthase isoform X1"
                     /calculated_mol_wt=30697
     Region          46..239
                     /region_name="GstA"
                     /note="Glutathione S-transferase [Posttranslational
                     modification, protein turnover, chaperones]; COG0625"
                     /db_xref="CDD:440390"
     CDS             1..264
                     /gene="LOC117600379"
                     /coded_by="XM_076689965.1:250..1044"
                     /db_xref="GeneID:117600379"
ORIGIN      
        1 msirtgilhn arsfykmptl ntlpvtyiyc vmhdspkppk iegqarlysm kfcpfsqrir
       61 lilsykniph dvvninlknk pewyseihpe gkvptfvdad gkvvtdslki anyldekype
      121 palynekakd rdlelldhys kivgvfsnci hdkdkrplse ivteissllv efeeelkarg
      181 ttffggsqpg mldilmwpwv errkalpliy keptnfdhen fphilkwmqe mkvqpfvqen
      241 ecshekfaes ienmragkld ydkl
//
LOCUS       XP_034171645             239 aa            linear   INV 03-SEP-2025
DEFINITION  pyrimidodiazepine synthase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034171645
VERSION     XP_034171645.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315754.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="pyrimidodiazepine synthase isoform X2"
                     /calculated_mol_wt=27712
     Region          2..90
                     /region_name="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold"
                     /note="The thioredoxin (TRX)-like superfamily is a large,
                     diverse group of proteins containing a TRX fold. Many
                     members contain a classic TRX domain with a redox active
                     CXXC motif. They function as protein disulfide
                     oxidoreductases (PDOs), altering the redox...; cl00388"
                     /db_xref="CDD:469754"
     Region          21..214
                     /region_name="GstA"
                     /note="Glutathione S-transferase [Posttranslational
                     modification, protein turnover, chaperones]; COG0625"
                     /db_xref="CDD:440390"
     CDS             1..239
                     /gene="LOC117600379"
                     /coded_by="XM_034315754.2:159..878"
                     /db_xref="GeneID:117600379"
ORIGIN      
        1 msdkhlgtds pkppkiegqa rlysmkfcpf sqrirlilsy kniphdvvni nlknkpewys
       61 eihpegkvpt fvdadgkvvt dslkianyld ekypepalyn ekakdrdlel ldhyskivgv
      121 fsncihdkdk rplseivtei ssllvefeee lkargttffg gsqpgmldil mwpwverrka
      181 lpliykeptn fdhenfphil kwmqemkvqp fvqenecshe kfaesienmr agkldydkl
//
LOCUS       XP_034171585             332 aa            linear   INV 03-SEP-2025
DEFINITION  transaldolase [Osmia lignaria lignaria].
ACCESSION   XP_034171585
VERSION     XP_034171585.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315694.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..332
                     /product="transaldolase"
                     /calculated_mol_wt=37008
     Region          11..331
                     /region_name="PRK05269"
                     /note="transaldolase B; Provisional"
                     /db_xref="CDD:235381"
     Site            order(27,43,45..46,104,106,142,165,167,187,192)
                     /site_type="active"
                     /db_xref="CDD:188644"
     Site            order(114,292,295,299..300,306,309,313)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:188644"
     Site            142
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:188644"
     CDS             1..332
                     /gene="Taldo"
                     /coded_by="XM_034315694.2:28..1026"
                     /db_xref="GeneID:117600352"
ORIGIN      
        1 msepqskkvk mtsslaqlke ittivadtgd fqameqfkpi dattnpslil aaanqkkyah
       61 lldkavqygk kfgstlaeqi eaalditcvl fgkeilniip grvstevdar lsfnkeasie
      121 kakrlialye elgvnkdrvl iklastwegi qaakeleeky gihcnltllf sfaqavacae
      181 agvtlispfv grildwyvsn tdkksyepke dpgvvsvtki ynyykkfgyk tvvmgasfrn
      241 vgeirelagc dfltispkll eeleksnepi rkvlapelak ksdlqkisln eakfrwllne
      301 dqmatdklsd girkfavdvr klekllqeki qs
//
LOCUS       XP_034171660             142 aa            linear   INV 03-SEP-2025
DEFINITION  thioredoxin-like protein Dim1 [Osmia lignaria lignaria].
ACCESSION   XP_034171660
VERSION     XP_034171660.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..142
                     /product="thioredoxin-like protein Dim1"
                     /calculated_mol_wt=16727
     Region          1..142
                     /region_name="PLN00410"
                     /note="U5 snRNP protein, DIM1 family; Provisional"
                     /db_xref="CDD:215109"
     Site            order(21,23..24,86..88)
                     /site_type="active"
                     /note="first critical site [active]"
                     /db_xref="CDD:239252"
     Site            40..43
                     /site_type="active"
                     /note="second critical site [active]"
                     /db_xref="CDD:239252"
     CDS             1..142
                     /gene="Dim1"
                     /coded_by="XM_034315769.2:388..816"
                     /db_xref="GeneID:117600381"
ORIGIN      
        1 msymlshlhn gwqvdqails eedrvvvirf ghdwdpmcmk mdevlyniae kvknfaviyl
       61 vditqvpdfn kmyelydpct vmfffrnkhi midlgtgnnn kinwtledkq emidiietvy
      121 rgarkgrglv vspkdystky ry
//
LOCUS       XP_034173429             508 aa            linear   INV 03-SEP-2025
DEFINITION  glycerol kinase 5 [Osmia lignaria lignaria].
ACCESSION   XP_034173429
VERSION     XP_034173429.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317538.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="glycerol kinase 5"
                     /calculated_mol_wt=56165
     Region          3..502
                     /region_name="ASKHA_NBD_FGGY_GK5-like"
                     /note="nucleotide-binding domain (NBD) of metazoan
                     glycerol kinase 5 (GK5) and similar proteins; cd07793"
                     /db_xref="CDD:466803"
     CDS             1..508
                     /gene="LOC117601135"
                     /coded_by="XM_034317538.2:217..1743"
                     /db_xref="GeneID:117601135"
ORIGIN      
        1 mkyigaldvg tttvrfhivd eqattvassv ekvqllypnp ghveidpdel wriivdvikn
       61 tlkdsgikae sivslgistq rgsfttwnlk dgrhyhnfit wkdvradsmv kewnssismk
      121 glrlgsqily tltrrkrfla asvfkfmntq mtlkllwalq hvpglqeatn ngnavfggld
      181 cwllykltgr hvtdassasa tglfdpftms wssvlinllk lpcnifpevl dttgnfgvtp
      241 keifdveipv lcsmadqsas lfgsgciqpg dlkitmgtgt flnvntgakp hasisglypl
      301 vgwridnevv yavegasndt gvlvewakki giintadeta siansvddsd gvyfvpafsg
      361 lqapindysa atgfigikpt teknhivrsl lesivyrill lyeslcaetc ftyhrirvdg
      421 gvsrndfilq lladltglev eratsiemsv lgvsflaglq cgvwtdqedv lklrktekif
      481 ipneenrlky qpiiaqwkra lqrlsqwy
//
LOCUS       XP_034175980            1397 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602280 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175980
VERSION     XP_034175980.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320089.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175980.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1397
                     /product="uncharacterized protein LOC117602280 isoform X1"
                     /calculated_mol_wt=161180
     Region          <157..981
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          833..>1141
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1397
                     /gene="LOC117602280"
                     /coded_by="XM_034320089.2:537..4730"
                     /db_xref="GeneID:117602280"
ORIGIN      
        1 mhhmysskkg dplcplgfhp qvrwptrckr cfrdykehgg kkdnlrdits stpslsfeqs
       61 sgrsaengkr vwssatnlak ddfkgssess paaagwtslm dlsaidkeee rlnarrptkl
      121 qirevianrg ggssendvef iiqvkkskig ssksaiqned hlmekeette ksemdrlraq
      181 lvelqarcek aekekseilm rrlttmetis nkassnelqk lqkknealmq ektnlmskvk
      241 eleketgskl frgdrdreke elrsklkaae nlcetlmden edmkkeirql eeeiyelqdt
      301 frdeqadeyv rlrksleqsn kncrilsfkl rkverkveel eseksglekk ydevkkveet
      361 lkkvkgefqg rpqkkatdlt skvqlkkmve emekeigdlm tiftnisdgk dvdvqelksk
      421 dsyikydklm keyellkekl dnvtkelsne kekkrtqsgs kaedkntdlq nikkkldeav
      481 nmreterkaw dqektalsee keklksklls lsaeklkvyn evvqlkkdle takssekege
      541 kmektinelk relsqerekn kklqddlsay tereskmtqs mtsveqtksk ldaevkrlkk
      601 elenakssns tkinnlttel selkkekekl lsqidqekqs keaevsmlkk kvnslektgl
      661 nakrmnemrq tynekilmay isdledelkk geqeynnlne kyielgklkt qfeadnealn
      721 nklreqntel inirkeletl rqtvklkese wrsekgvlqn klkeselpnr amitdlnndi
      781 snlkkenntl vtrledlrka nddlsdklkd yeavskihqa itpdtnales eirklknale
      841 nmekakkadl aqckmryehr itaindeiqt iqnqlsrykr erdtykhmle gaqktigelk
      901 snrgrlasna ssgksdeeee msgasrlile rqinsledel setrletsrl kaelvseksa
      961 shvkvselqs rineleeerm iasgrtkipg lkvrmelawq kereehqrll qetatlardl
     1021 rqtlfeiere rskerlenkr rqdqlkkvyd eekeeskkkl lelqcdllel rdahaklrts
     1081 nekmrreker herereelkd aifkkskqeq telrninvll qqvndlmkff pelngiaeng
     1141 nsstytptpp rrlkgpksre sspmldakgd irgsnsqlge rtekleytik klmdvakelk
     1201 eskkaadetn atrlkklskr stsvesdpgk gittarskpr lkrkslsleq ttarneesri
     1261 wgtdsnmssl qslegsevdg rtlslqrdss vdsrlstgst ksemlerekk hgkgiikklt
     1321 tkltksasvd dpnvsmdlsl qtsgsetsit ektekknlkk kltdifkrgs rgssvekktg
     1381 tssqsrpvsr nsttsdk
//
LOCUS       XP_034175988            1397 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602280 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175988
VERSION     XP_034175988.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320097.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175988.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1397
                     /product="uncharacterized protein LOC117602280 isoform X1"
                     /calculated_mol_wt=161180
     Region          <157..981
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          833..>1141
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1397
                     /gene="LOC117602280"
                     /coded_by="XM_034320097.2:380..4573"
                     /db_xref="GeneID:117602280"
ORIGIN      
        1 mhhmysskkg dplcplgfhp qvrwptrckr cfrdykehgg kkdnlrdits stpslsfeqs
       61 sgrsaengkr vwssatnlak ddfkgssess paaagwtslm dlsaidkeee rlnarrptkl
      121 qirevianrg ggssendvef iiqvkkskig ssksaiqned hlmekeette ksemdrlraq
      181 lvelqarcek aekekseilm rrlttmetis nkassnelqk lqkknealmq ektnlmskvk
      241 eleketgskl frgdrdreke elrsklkaae nlcetlmden edmkkeirql eeeiyelqdt
      301 frdeqadeyv rlrksleqsn kncrilsfkl rkverkveel eseksglekk ydevkkveet
      361 lkkvkgefqg rpqkkatdlt skvqlkkmve emekeigdlm tiftnisdgk dvdvqelksk
      421 dsyikydklm keyellkekl dnvtkelsne kekkrtqsgs kaedkntdlq nikkkldeav
      481 nmreterkaw dqektalsee keklksklls lsaeklkvyn evvqlkkdle takssekege
      541 kmektinelk relsqerekn kklqddlsay tereskmtqs mtsveqtksk ldaevkrlkk
      601 elenakssns tkinnlttel selkkekekl lsqidqekqs keaevsmlkk kvnslektgl
      661 nakrmnemrq tynekilmay isdledelkk geqeynnlne kyielgklkt qfeadnealn
      721 nklreqntel inirkeletl rqtvklkese wrsekgvlqn klkeselpnr amitdlnndi
      781 snlkkenntl vtrledlrka nddlsdklkd yeavskihqa itpdtnales eirklknale
      841 nmekakkadl aqckmryehr itaindeiqt iqnqlsrykr erdtykhmle gaqktigelk
      901 snrgrlasna ssgksdeeee msgasrlile rqinsledel setrletsrl kaelvseksa
      961 shvkvselqs rineleeerm iasgrtkipg lkvrmelawq kereehqrll qetatlardl
     1021 rqtlfeiere rskerlenkr rqdqlkkvyd eekeeskkkl lelqcdllel rdahaklrts
     1081 nekmrreker herereelkd aifkkskqeq telrninvll qqvndlmkff pelngiaeng
     1141 nsstytptpp rrlkgpksre sspmldakgd irgsnsqlge rtekleytik klmdvakelk
     1201 eskkaadetn atrlkklskr stsvesdpgk gittarskpr lkrkslsleq ttarneesri
     1261 wgtdsnmssl qslegsevdg rtlslqrdss vdsrlstgst ksemlerekk hgkgiikklt
     1321 tkltksasvd dpnvsmdlsl qtsgsetsit ektekknlkk kltdifkrgs rgssvekktg
     1381 tssqsrpvsr nsttsdk
//
LOCUS       XP_034175998            1397 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602280 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175998
VERSION     XP_034175998.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320107.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175998.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1397
                     /product="uncharacterized protein LOC117602280 isoform X1"
                     /calculated_mol_wt=161180
     Region          <157..981
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          833..>1141
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1397
                     /gene="LOC117602280"
                     /coded_by="XM_034320107.2:622..4815"
                     /db_xref="GeneID:117602280"
ORIGIN      
        1 mhhmysskkg dplcplgfhp qvrwptrckr cfrdykehgg kkdnlrdits stpslsfeqs
       61 sgrsaengkr vwssatnlak ddfkgssess paaagwtslm dlsaidkeee rlnarrptkl
      121 qirevianrg ggssendvef iiqvkkskig ssksaiqned hlmekeette ksemdrlraq
      181 lvelqarcek aekekseilm rrlttmetis nkassnelqk lqkknealmq ektnlmskvk
      241 eleketgskl frgdrdreke elrsklkaae nlcetlmden edmkkeirql eeeiyelqdt
      301 frdeqadeyv rlrksleqsn kncrilsfkl rkverkveel eseksglekk ydevkkveet
      361 lkkvkgefqg rpqkkatdlt skvqlkkmve emekeigdlm tiftnisdgk dvdvqelksk
      421 dsyikydklm keyellkekl dnvtkelsne kekkrtqsgs kaedkntdlq nikkkldeav
      481 nmreterkaw dqektalsee keklksklls lsaeklkvyn evvqlkkdle takssekege
      541 kmektinelk relsqerekn kklqddlsay tereskmtqs mtsveqtksk ldaevkrlkk
      601 elenakssns tkinnlttel selkkekekl lsqidqekqs keaevsmlkk kvnslektgl
      661 nakrmnemrq tynekilmay isdledelkk geqeynnlne kyielgklkt qfeadnealn
      721 nklreqntel inirkeletl rqtvklkese wrsekgvlqn klkeselpnr amitdlnndi
      781 snlkkenntl vtrledlrka nddlsdklkd yeavskihqa itpdtnales eirklknale
      841 nmekakkadl aqckmryehr itaindeiqt iqnqlsrykr erdtykhmle gaqktigelk
      901 snrgrlasna ssgksdeeee msgasrlile rqinsledel setrletsrl kaelvseksa
      961 shvkvselqs rineleeerm iasgrtkipg lkvrmelawq kereehqrll qetatlardl
     1021 rqtlfeiere rskerlenkr rqdqlkkvyd eekeeskkkl lelqcdllel rdahaklrts
     1081 nekmrreker herereelkd aifkkskqeq telrninvll qqvndlmkff pelngiaeng
     1141 nsstytptpp rrlkgpksre sspmldakgd irgsnsqlge rtekleytik klmdvakelk
     1201 eskkaadetn atrlkklskr stsvesdpgk gittarskpr lkrkslsleq ttarneesri
     1261 wgtdsnmssl qslegsevdg rtlslqrdss vdsrlstgst ksemlerekk hgkgiikklt
     1321 tkltksasvd dpnvsmdlsl qtsgsetsit ektekknlkk kltdifkrgs rgssvekktg
     1381 tssqsrpvsr nsttsdk
//
LOCUS       XP_034176004            1392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602280 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176004
VERSION     XP_034176004.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320113.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176004.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1392
                     /product="uncharacterized protein LOC117602280 isoform X2"
                     /calculated_mol_wt=160613
     Region          <175..>373
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          281..842
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          828..>1136
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1392
                     /gene="LOC117602280"
                     /coded_by="XM_034320113.2:536..4714"
                     /db_xref="GeneID:117602280"
ORIGIN      
        1 mhhmysskkg dplcplgfhp qvrwptrckr cfrdykehgg kkdnlrdits stpslsfeqs
       61 sgrsaengkr vwssatnlak ddfkgssess paaagwtslm dlsaidkeee rlnarrptkl
      121 qirevianrg ggssendvef iiqvkkskig ssksaiqned hlmekeette ksemdrlraq
      181 lvelqarcek aekekseilm rrlttmetis nkassnelqk lqkknealmq ektnlmskvk
      241 eleketgskl frgdrdreke elrsklkaae nlcetlmden edmkkeirql eeeiyelqdt
      301 frdeqadeyv rlrksleqsn kncrilsfkl rkverkveel eseksglekk ydevkkveet
      361 lkkvkgefqg rpqkkatdlt skvqlkkmve emekeigdlm tiftnisdgk dvdvqelksk
      421 dsyikydklm keyellkekl dnvtkelsne kekkrtqsgs kaedkntdlq nikkkldeav
      481 nmreterkaw dqektalsee keklksklls lsaeklkvyn evvqlkkdle takssekege
      541 kmektinelk relsqerekn kklqddlsay tereskmtqs mtsveqtksk ldaevkrlkk
      601 elenakssns tkinnlttel selkkekekl lsqidqekqs keaevsmlkk kvnslektgl
      661 nakrmnemrq tynekilnle delkkgeqey nnlnekyiel gklktqfead nealnnklre
      721 qntelinirk eletlrqtvk lkesewrsek gvlqnklkes elpnramitd lnndisnlkk
      781 enntlvtrle dlrkanddls dklkdyeavs kihqaitpdt naleseirkl knalenmeka
      841 kkadlaqckm ryehritain deiqtiqnql srykrerdty khmlegaqkt igelksnrgr
      901 lasnassgks deeeemsgas rlilerqins ledelsetrl etsrlkaelv seksashvkv
      961 selqsrinel eeermiasgr tkipglkvrm elawqkeree hqrllqetat lardlrqtlf
     1021 eierersker lenkrrqdql kkvydeekee skkkllelqc dllelrdaha klrtsnekmr
     1081 rekerherer eelkdaifkk skqeqtelrn invllqqvnd lmkffpelng iaengnssty
     1141 tptpprrlkg pksresspml dakgdirgsn sqlgertekl eytikklmdv akelkeskka
     1201 adetnatrlk klskrstsve sdpgkgitta rskprlkrks lsleqttarn eesriwgtds
     1261 nmsslqsleg sevdgrtlsl qrdssvdsrl stgstkseml erekkhgkgi ikklttkltk
     1321 sasvddpnvs mdlslqtsgs etsitektek knlkkkltdi fkrgsrgssv ekktgtssqs
     1381 rpvsrnstts dk
//
LOCUS       XP_034176013            1369 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602280 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176013
VERSION     XP_034176013.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176013.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1369
                     /product="uncharacterized protein LOC117602280 isoform X3"
                     /calculated_mol_wt=158022
     Region          <150..953
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          805..>1113
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1369
                     /gene="LOC117602280"
                     /coded_by="XM_034320122.2:536..4645"
                     /db_xref="GeneID:117602280"
ORIGIN      
        1 mhhmysskkg dplcplgfhp qvrwptrckr cfrdykehgg kkdnlrdits stpslsfeqs
       61 sgrsaengkr vwssatnlak ddfkgssess paaagwtslm dlsaidkeee rlnarrptkl
      121 qirevianrg ggssendvef iiqsemdrlr aqlvelqarc ekaekeksei lmrrlttmet
      181 isnkassnel qklqkkneal mqektnlmsk vkeleketgs klfrgdrdre keelrsklka
      241 aenlcetlmd enedmkkeir qleeeiyelq dtfrdeqade yvrlrksleq snkncrilsf
      301 klrkverkve eleseksgle kkydevkkve etlkkvkgef qgrpqkkatd ltskvqlkkm
      361 veemekeigd lmtiftnisd gkdvdvqelk skdsyikydk lmkeyellke kldnvtkels
      421 nekekkrtqs gskaedkntd lqnikkklde avnmreterk awdqektals eekeklkskl
      481 lslsaeklkv ynevvqlkkd letaksseke gekmektine lkrelsqere knkklqddls
      541 aytereskmt qsmtsveqtk skldaevkrl kkelenakss nstkinnltt elselkkeke
      601 kllsqidqek qskeaevsml kkkvnslekt glnakrmnem rqtynekilm ayisdledel
      661 kkgeqeynnl nekyielgkl ktqfeadnea lnnklreqnt elinirkele tlrqtvklke
      721 sewrsekgvl qnklkeselp nramitdlnn disnlkkenn tlvtrledlr kanddlsdkl
      781 kdyeavskih qaitpdtnal eseirklkna lenmekakka dlaqckmrye hritaindei
      841 qtiqnqlsry krerdtykhm legaqktige lksnrgrlas nassgksdee eemsgasrli
      901 lerqinsled elsetrlets rlkaelvsek sashvkvsel qsrineleee rmiasgrtki
      961 pglkvrmela wqkereehqr llqetatlar dlrqtlfeie rerskerlen krrqdqlkkv
     1021 ydeekeeskk kllelqcdll elrdahaklr tsnekmrrek erherereel kdaifkkskq
     1081 eqtelrninv llqqvndlmk ffpelngiae ngnsstytpt pprrlkgpks resspmldak
     1141 gdirgsnsql gertekleyt ikklmdvake lkeskkaade tnatrlkkls krstsvesdp
     1201 gkgittarsk prlkrkslsl eqttarnees riwgtdsnms slqslegsev dgrtlslqrd
     1261 ssvdsrlstg stksemlere kkhgkgiikk lttkltksas vddpnvsmdl slqtsgsets
     1321 itektekknl kkkltdifkr gsrgssvekk tgtssqsrpv srnsttsdk
//
LOCUS       XP_076546081            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546081
VERSION     XP_076546081.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689966.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689966.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546082            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546082
VERSION     XP_076546082.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689967.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689967.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546083            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546083
VERSION     XP_076546083.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689968.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689968.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546084            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546084
VERSION     XP_076546084.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689969.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689969.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546085            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546085
VERSION     XP_076546085.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689970.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689970.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546086            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546086
VERSION     XP_076546086.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689971.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689971.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546087            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546087
VERSION     XP_076546087.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689972.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689972.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546088            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546088
VERSION     XP_076546088.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689973.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689973.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546089            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546089
VERSION     XP_076546089.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689974.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689974.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546090            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546090
VERSION     XP_076546090.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689975.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689975.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_076546091            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_076546091
VERSION     XP_076546091.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689976.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_076689976.1:502..4182"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_034176027            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_034176027
VERSION     XP_034176027.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320136.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176027.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_034320136.2:433..4113"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_034176047            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602303 [Osmia lignaria lignaria].
ACCESSION   XP_034176047
VERSION     XP_034176047.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320156.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176047.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117602303"
                     /calculated_mol_wt=139745
     Region          964..1018
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(966,969,982,986,989,992,1010,1014)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          1049..1098
                     /region_name="BRcat_RBR_RNF216"
                     /note="BRcat domain found in RING finger protein 216
                     (RNF216); cd20339"
                     /db_xref="CDD:439000"
     Region          1109..1169
                     /region_name="Rcat_RBR_RNF216"
                     /note="Rcat domain found in RING finger protein 216
                     (RNF216); cd20353"
                     /db_xref="CDD:439014"
     CDS             1..1226
                     /gene="LOC117602303"
                     /coded_by="XM_034320156.2:242..3922"
                     /db_xref="GeneID:117602303"
ORIGIN      
        1 mdveellsse scsdslsnnf sahtdddddd iicnylnaqq afikksvspl sadlvavidt
       61 yddtpvsitf dstqdesdii ileennsnsr inlnstqsfq rqlkltavsq nnthetdfvr
      121 clrsltpngs nnlhdsasnd fdkkireevl qicnllptfn yesvykvlhk nrnaknriel
      181 slwdllpnkr prvkhfedls tqakrlkqsd trinnmaqpv sngtlnsqta cssngsrnre
      241 tvfqancenr vpeysvedgk vssttgggil rpaklvindk wsclddiasr nsfimapskl
      301 tsvpakrlms gkatkssasv ekhennkltn qnvfvfnsik nkysrqifhk ymeyfkhmen
      361 ikkqkltgec nnfdfekrni trdiflktdw ltnidtqets nnvakpstsg vnfsvseekp
      421 vcggtskmkn krkenlirnk pvvtnialpf kflqmnerlv fmfpqadkis irqicknlsk
      481 lentvsmerl qtlaehtlva linfanlfvn ikacttsadt pvtaitdsnt slhqvvptnt
      541 ttpkqqsltf dlcrpststa vysapetnev iggarrkqii kkkedsksdk dvptntkilt
      601 qqslwktrwl ktvemverek rkqaiekeds ksnknvptnt ttlrqqslin kfdlrpstsi
      661 avysatnkvi ggarkkqiie kegsesnkna kkknivlplk feemyetlvl mfpqmdkdfi
      721 rqtcknvskv egvcsfeqlq tlaehtlqnk eiqsaeidvr slcpekdenn qyiyllgifp
      781 dadptylkkv vartqdnpse iinfiqsqwe kptyptkeek lrkktitqqq iqytskfdvk
      841 qfleifpdpl shftnpkrtc ksssdavefl kshfthfees ylkdvykwsn ynltltvqkl
      901 enttpyiklt rnirsldkcl tldipllqef afithrqeiv ehldilkete mlkfaelkak
      961 nellecqccy dnecmpskcs tcddghifcn scilkgtesq lsqgetrisc fsncdgefti
     1021 ptlqkilppt qfsifirkkq eaevmaakve glvscpfchf asvpppedkv fkclnpecmk
     1081 escrlckqpn hiplrcyeek sdqarlflee kmtealvrkc ykcsrpyfkd dgcnkitcsc
     1141 gammcyicdi piysydhfgt grcpqwsdnl iinnetvrav aektvelikk kdpnvtvnad
     1201 dllkylpsts ngairgrvek ivtfdk
//
LOCUS       XP_034176088             955 aa            linear   INV 03-SEP-2025
DEFINITION  G patch domain-containing protein 1 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034176088
VERSION     XP_034176088.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320197.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176088.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..955
                     /product="G patch domain-containing protein 1 homolog"
                     /calculated_mol_wt=107073
     Region          29..113
                     /region_name="DUF1604"
                     /note="Protein of unknown function (DUF1604); pfam07713"
                     /db_xref="CDD:462241"
     Region          146..184
                     /region_name="G-patch"
                     /note="G-patch domain; cl02611"
                     /db_xref="CDD:445852"
     CDS             1..955
                     /gene="LOC117602326"
                     /coded_by="XM_034320197.2:276..3143"
                     /db_xref="GeneID:117602326"
ORIGIN      
        1 msdsedenyv sfgvpldpld ednlprkkpv tieeqcayda qgrrrfhgaf tggfsagyfn
       61 tvgtrdgwrp qhfkssrssk aggitqcped fmdeedtgqf giapkgicat sdytdhghrg
      121 vkrerinres sgpipgtpvl kdllkpvket vgimllkkmg wrpgqgigsr vtkkekakik
      181 rrnakmkvlq qdskkanses ssedseddhe dvtfapddye pfrcnpkdny fgigysgldr
      241 rtilsghvnl fdtpafsvqd knkklsihgq afgvgafead dediyeredm srydfslgpe
      301 rktktrwssd nslkndncle gfalasaklq hkkifpppel pkdfvpmhav rksrfypptd
      361 nipraiengk rkeltaedra rileetpssk klsesypnsv psvasniitk vlnlhgkqrt
      421 eerqreenqr vkaatswmdk lnaqsfvkgg tvglgkdseg slknleefkd sfsaseeqsg
      481 tnnliedssi nkstmkpfls dpdkqrrfeq ylvfsdkgek dklesiqsls mtewdreher
      541 vefeqaaklf vkstneyvts kfthasesvs sdassakneq dnelkqavkm kmfgkltrer
      601 iewkpasivc krfnipepqg gcappemqkk takfsifdsi dwsnstkflr atdtvsketl
      661 estapskeds vinntnvdik snkdipvnsq qaiepvtekm rnfeasyekv fgkevqetfp
      721 ratevkenlg nkfdstteip deqakdvend sitkskseek kdlfkaifls ssdeesdsep
      781 eesvdsevvk svligkvpse vnvnrntspp rgifakvdld slvadprsst qssetkkevd
      841 vitvsdtdsg skqisdtgsn ppevqllpdm ygpalpsrll kneappepea sssqtfkpvf
      901 rsvvvpkpkt dskvtgvwve rtkvkkskke kkkhkhkeqk sskhkrkskk ekrgp
//
LOCUS       XP_034176162             580 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquinone biosynthesis protein COQ9, mitochondrial isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034176162
VERSION     XP_034176162.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320271.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="ubiquinone biosynthesis protein COQ9,
                     mitochondrial isoform X1"
                     /calculated_mol_wt=67128
     Region          25..496
                     /region_name="Kinetochor_Ybp2"
                     /note="Uncharacterized protein family, YAP/Alf4/glomulin;
                     pfam08568"
                     /db_xref="CDD:400745"
     CDS             1..580
                     /gene="Coq9"
                     /coded_by="XM_034320271.2:576..2318"
                     /db_xref="GeneID:117602363"
ORIGIN      
        1 mtdpkenvtn kfindltdyl kknklkevln lfednsydni iqesswyivp ivsshltnen
       61 tkynaeliec ctkilniive kcnpsetvle lleqvegpei hvkfcivlkv lnkclnkmnn
      121 kmkaiewcss tirsyvdslp ipdedspsst itinkiqniy iviisflepl veearlense
      181 kcatlkdyla siliflmgkp lshlpekylk qsipekivtl vsqitgdllw flnivnlrsk
      241 ktnskkkcie eechnlkvtl feldenisdl ayanfyfyvi trihlwervp qvydfqyifq
      301 aciyliikll edqetiakgl slmdqllkrl irrsltsell elniylqlfd tiakvmicyf
      361 nnnkerkral iifqeyvemf dmqarylivf rlyqtskysg llslttgivk dsvieclqsv
      421 plipyflgnn lesliklack lpherasdlv elsdeiitsl nllrflfird qqnqtgiwnl
      481 vdelekdylq plregiylcr thwkvkvkdv eeqkkaikan aktelernvi eaplpvggek
      541 lsaipvlnkl slcyqavngl dvmesilirv necimnnplk
//
LOCUS       XP_034176170             469 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquinone biosynthesis protein COQ9, mitochondrial isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034176170
VERSION     XP_034176170.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320279.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="ubiquinone biosynthesis protein COQ9,
                     mitochondrial isoform X2"
                     /calculated_mol_wt=54342
     Region          <223..385
                     /region_name="Kinetochor_Ybp2"
                     /note="Uncharacterized protein family, YAP/Alf4/glomulin;
                     pfam08568"
                     /db_xref="CDD:400745"
     CDS             1..469
                     /gene="Coq9"
                     /coded_by="XM_034320279.2:147..1556"
                     /db_xref="GeneID:117602363"
ORIGIN      
        1 mtsslfkige ygyvkyfvna krvfserlpi pdedspssti tinkiqniyi viisfleplv
       61 eearlensek catlkdylas iliflmgkpl shlpekylkq sipekivtlv sqitgdllwf
      121 lnivnlrskk tnskkkciee echnlkvtlf eldenisdla yanfyfyvit rihlwervpq
      181 vydfqyifqa ciyliiklle dqetiakgls lmdqllkrli rrsltselle lniylqlfdt
      241 iakvmicyfn nnkerkrali ifqeyvemfd mqarylivfr lyqtskysgl lslttgivkd
      301 svieclqsvp lipyflgnnl esliklackl pherasdlve lsdeiitsln llrflfirdq
      361 qnqtgiwnlv delekdylqp lregiylcrt hwkvkvkdve eqkkaikana ktelernvie
      421 aplpvggekl saipvlnkls lcyqavngld vmesilirvn ecimnnplk
//
LOCUS       XP_034176179             284 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquinone biosynthesis protein COQ9, mitochondrial isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034176179
VERSION     XP_034176179.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320288.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="ubiquinone biosynthesis protein COQ9,
                     mitochondrial isoform X3"
                     /calculated_mol_wt=32105
     Region          59..242
                     /region_name="COQ9"
                     /note="cl21709"
                     /db_xref="CDD:473940"
     CDS             1..284
                     /gene="Coq9"
                     /coded_by="XM_034320288.2:148..1002"
                     /db_xref="GeneID:117602363"
ORIGIN      
        1 mamssillmr kgflangfrs lwtcktllsn qtenantqqt skeytnckes deeyeknikt
       61 kilaaslkyv hdlgwsqqai sagaesigyp gvvhglfpnk gvdlvqyfyl tcntelneil
      121 mkraeateen ctkgekples qvrdaveirl rmvipykktw pqalalmalp pnapkslanl
      181 ltlvddicyy agdrsvdinw ytrrvvlagi ykttelymlq dnsedhrqtw nflerrikda
      241 tqihaffttt sdialpdqal nrvsetasaa fvtarnilgl nwnr
//
LOCUS       XP_034176191             511 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602376 [Osmia lignaria lignaria].
ACCESSION   XP_034176191
VERSION     XP_034176191.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320300.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="uncharacterized protein LOC117602376"
                     /calculated_mol_wt=55876
     Region          128..170
                     /region_name="F-box_FBXL16"
                     /note="F-box domain found in F-box/LRR-repeat protein 16
                     (FBXL16) and similar proteins; cd22127"
                     /db_xref="CDD:438899"
     Site            order(130,134,137..138,141..142,146,148..149,153..155,157)
                     /site_type="other"
                     /note="putative Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438899"
     Region          227..251
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          237..445
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          252..273
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          276..301
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          302..327
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          328..353
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          354..379
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          380..405
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          406..430
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          431..455
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..511
                     /gene="LOC117602376"
                     /coded_by="XM_034320300.2:44..1579"
                     /db_xref="GeneID:117602376"
ORIGIN      
        1 mssisaqgvv erasaeltkr inglglrtsk hhgggkasvm ervtnvfcgs ggvaytnlqs
       61 annanatpek psrsvptsms nmpaannksl nssntpsrar isrnrpknvp lasggtggyv
      121 apatwdqlmq ddhflgkffl yftaverril aqvctrwrdi lyarprlwag lvpvircrev
      181 ralppnsrtr lyaslvrrgf hslvllgasd edipdlthgf plaqrnihsl slrccavtdr
      241 gletlldhlq alfelelagc neiteaglwt cltprivsls lsdcinvade avgavaqllp
      301 slyefslqay hvtdaalsyf hasqssslsi lrlqscwelt nhgvvnivhs lpnltvlsls
      361 gcskvtddgv eliaenlsrl rsldlswcsr itdaaleyia cdlnhleelt ldrcvhitdi
      421 gvgyistmgs lsalflrwci llrdfglqhl cgmkslqvls vagcplltss glssliqlrh
      481 lheleltncp gtsqelfdyl rehlprclii e
//
LOCUS       XP_034181255             494 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034181255
VERSION     XP_034181255.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325364.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181255.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="tyrosine-protein phosphatase non-receptor type 2
                     isoform X1"
                     /calculated_mol_wt=55558
     Region          56..286
                     /region_name="PTPc-N1_2"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 1 and type 2; cd14545"
                     /db_xref="CDD:350393"
     CDS             1..494
                     /gene="LOC117604848"
                     /coded_by="XM_034325364.2:331..1815"
                     /db_xref="GeneID:117604848"
ORIGIN      
        1 mtsqetceqg isnveteyhe inskeawpil yqhirnecsn fsytynesrk pqnknlnryr
       61 dvlpydhsri vlkrgpcdyi nanliqvdra hrqyiltqgp lentaghfwl mvweqnskav
      121 lmlnkiiekn hvkcyqywpl gdsennntmm fpdvglkvtf vsktessdyt trvlkltdle
      181 tnesreilhf hyttwpdfgv pqsptaflsf lthvrqsgal dqnvgppvvh csagigrsgt
      241 fclvdtclvl igenglnsvn vrdiliemrr yrmgliqtpg qlrfsyaaii egakqllsnn
      301 vninnevvtn hydvvsnlnn stveeedepp plppprgesl trsmmadlts npvtnkndel
      361 ggppdkplpr epsaptetst sspvanstvi tnnlheknsd gvvvpnents dgenslqtis
      421 ppsspdtkne vrhrnkekke rlaaqvremk rrqketeewq klkrslfkpl sigigaaivg
      481 ggiiaicgyl ymrg
//
LOCUS       XP_076546092             493 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546092
VERSION     XP_076546092.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689977.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="tyrosine-protein phosphatase non-receptor type 2
                     isoform X2"
                     /calculated_mol_wt=55459
     Region          56..286
                     /region_name="PTPc-N1_2"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 1 and type 2; cd14545"
                     /db_xref="CDD:350393"
     CDS             1..493
                     /gene="LOC117604848"
                     /coded_by="XM_076689977.1:330..1811"
                     /db_xref="GeneID:117604848"
ORIGIN      
        1 mtsqetceqg isnveteyhe inskeawpil yqhirnecsn fsytynesrk pqnknlnryr
       61 dvlpydhsri vlkrgpcdyi nanliqvdra hrqyiltqgp lentaghfwl mvweqnskav
      121 lmlnkiiekn hvkcyqywpl gdsennntmm fpdvglkvtf vsktessdyt trvlkltdle
      181 tnesreilhf hyttwpdfgv pqsptaflsf lthvrqsgal dqnvgppvvh csagigrsgt
      241 fclvdtclvl igenglnsvn vrdiliemrr yrmgliqtpg qlrfsyaaii egakqllsnn
      301 ninnevvtnh ydvvsnlnns tveeedeppp lppprgeslt rsmmadltsn pvtnkndelg
      361 gppdkplpre psaptetsts spvanstvit nnlheknsdg vvvpnentsd genslqtisp
      421 psspdtknev rhrnkekker laaqvremkr rqketeewqk lkrslfkpls igigaaivgg
      481 giiaicgyly mrg
//
LOCUS       XP_076546093             488 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 2 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546093
VERSION     XP_076546093.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689978.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..488
                     /product="tyrosine-protein phosphatase non-receptor type 2
                     isoform X3"
                     /calculated_mol_wt=55166
     Region          56..286
                     /region_name="PTPc-N1_2"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 1 and type 2; cd14545"
                     /db_xref="CDD:350393"
     CDS             1..488
                     /gene="LOC117604848"
                     /coded_by="XM_076689978.1:330..1796"
                     /db_xref="GeneID:117604848"
ORIGIN      
        1 mtsqetceqg isnveteyhe inskeawpil yqhirnecsn fsytynesrk pqnknlnryr
       61 dvlpydhsri vlkrgpcdyi nanliqvdra hrqyiltqgp lentaghfwl mvweqnskav
      121 lmlnkiiekn hvkcyqywpl gdsennntmm fpdvglkvtf vsktessdyt trvlkltdle
      181 tnesreilhf hyttwpdfgv pqsptaflsf lthvrqsgal dqnvgppvvh csagigrsgt
      241 fclvdtclvl igenglnsvn vrdiliemrr yrmgliqtpg qlrfsyaaii egakqllsnn
      301 vninnevvtn hydvvsnlnn stveeedepp plppprgesl trsmmadlts npvtnkndel
      361 ggppdkplpr epsaptetst sspvanstvi tnnlheknsd gvvvpnents dgenslqtis
      421 ppsspdtkne vrhrnkekke rlaaqvremk rrqketeewq klkrsksett ptttessetv
      481 heeaepei
//
LOCUS       XP_034176268             416 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602408 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176268
VERSION     XP_034176268.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320377.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="uncharacterized protein LOC117602408 isoform X1"
                     /calculated_mol_wt=47152
     Region          254..353
                     /region_name="SH2_SOCS6"
                     /note="Src homology 2 (SH2) domain found in suppressor of
                     cytokine signaling (SOCS) proteins; cd10387"
                     /db_xref="CDD:198250"
     Site            order(272,290,292,300,313)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198250"
     Site            order(323,325,342)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198250"
     Region          377..416
                     /region_name="SOCS_SOCS6"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS6-like proteins. Together with CIS1, the CIS/SOCS
                     family of proteins is characterized by the presence of a
                     C-terminal SOCS box and a central SH2 domain. The general
                     function of the SOCS box is the...; cd03740"
                     /db_xref="CDD:239709"
     Site            order(377..382,388,395,401,406)
                     /site_type="other"
                     /note="putative elongin B/C interaction [polypeptide
                     binding]"
                     /db_xref="CDD:239709"
     CDS             1..416
                     /gene="LOC117602408"
                     /coded_by="XM_034320377.2:258..1508"
                     /db_xref="GeneID:117602408"
ORIGIN      
        1 minqmseeti ataptsaekp snldtkprkl svktfdflwk rdsrrenilk kennqkkkdl
       61 iegkvieskt knapsnkrsf nvfkgikrhm qikkltihds kqkmketysl qndtncdsst
      121 lqqnkctvsv egstlsdnkc edikknsdil nssesltatd ectlsqnect ldnlneaveg
      181 csdietktes vhvqvvdnyq sdnnhetipn mhylfsnnnt lqiesvvaee hqvenieeis
      241 esninndkak vslteellkl snygwywgpi sgteadaklm sepdgaflvr dssddryvlt
      301 lsfkssgkll harmehsggl fslcnqsese gfssvadlin ysmnfsqsav fcysrpkypg
      361 hpsfpvrltk pvsrfnqvrs lqylcrfvir qntrldnihk lplpktikgy ieeahy
//
LOCUS       XP_034176277             408 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602408 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176277
VERSION     XP_034176277.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320386.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..408
                     /product="uncharacterized protein LOC117602408 isoform X2"
                     /calculated_mol_wt=46171
     Region          246..345
                     /region_name="SH2_SOCS6"
                     /note="Src homology 2 (SH2) domain found in suppressor of
                     cytokine signaling (SOCS) proteins; cd10387"
                     /db_xref="CDD:198250"
     Site            order(264,282,284,292,305)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198250"
     Site            order(315,317,334)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198250"
     Region          369..408
                     /region_name="SOCS_SOCS6"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS6-like proteins. Together with CIS1, the CIS/SOCS
                     family of proteins is characterized by the presence of a
                     C-terminal SOCS box and a central SH2 domain. The general
                     function of the SOCS box is the...; cd03740"
                     /db_xref="CDD:239709"
     Site            order(369..374,380,387,393,398)
                     /site_type="other"
                     /note="putative elongin B/C interaction [polypeptide
                     binding]"
                     /db_xref="CDD:239709"
     CDS             1..408
                     /gene="LOC117602408"
                     /coded_by="XM_034320386.2:259..1485"
                     /db_xref="GeneID:117602408"
ORIGIN      
        1 minqmseeti ataptsaekp snldtkprkl svktfdflwk rdsrrenilk kennqkkkdl
       61 iegkvieskt knapsnkrsf nvfkgikrhm qikkltihds kqkndtncds stlqqnkctv
      121 svegstlsdn kcedikknsd ilnsseslta tdectlsqne ctldnlneav egcsdietkt
      181 esvhvqvvdn yqsdnnheti pnmhylfsnn ntlqiesvva eehqveniee isesninndk
      241 akvslteell klsnygwywg pisgteadak lmsepdgafl vrdssddryv ltlsfkssgk
      301 llharmehsg glfslcnqse segfssvadl inysmnfsqs avfcysrpky pghpsfpvrl
      361 tkpvsrfnqv rslqylcrfv irqntrldni hklplpktik gyieeahy
//
LOCUS       XP_034176290             388 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602416 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176290
VERSION     XP_034176290.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320399.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..388
                     /product="uncharacterized protein LOC117602416 isoform X1"
                     /calculated_mol_wt=43848
     CDS             1..388
                     /gene="LOC117602416"
                     /coded_by="XM_034320399.2:127..1293"
                     /db_xref="GeneID:117602416"
ORIGIN      
        1 mnvlqylrii ttfqlilcli lilnggistc kplssedveg llpptprdkn etiaavvqdp
       61 vvqdavqkva nngslnglvv kknvfimpas npnmilskre hilvvptenl edvrknitet
      121 kqteaamets spesemsies dadeympvei esgpqqnidl qsspvenpgq smltneqlks
      181 ktefledais hdiplpedqp kkvpvpiiav idpskaekgk vnspivailp nqldndalkn
      241 qvqflndnnd viqkkaqdyi dqssftivpd yrksstpsei tldlssiqet vpvpvsswsf
      301 qqddslkpit msrwemivpa yqddnmdhsr ekgdmdvaed ivfrplfkyr qqmqqrskyy
      361 desnrryggy snnyprrayf yrprydny
//
LOCUS       XP_034176301             384 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602416 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176301
VERSION     XP_034176301.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320410.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="uncharacterized protein LOC117602416 isoform X2"
                     /calculated_mol_wt=43372
     CDS             1..384
                     /gene="LOC117602416"
                     /coded_by="XM_034320410.2:120..1274"
                     /db_xref="GeneID:117602416"
ORIGIN      
        1 mkhkmittfq lilclililn ggistckpls sedvegllpp tprdknetia avvqdpvvqd
       61 avqkvanngs lnglvvkknv fimpasnpnm ilskrehilv vptenledvr knitetkqte
      121 aametsspes emsiesdade ympveiesgp qqnidlqssp venpgqsmlt neqlksktef
      181 ledaishdip lpedqpkkvp vpiiavidps kaekgkvnsp ivailpnqld ndalknqvqf
      241 lndnndviqk kaqdyidqss ftivpdyrks stpseitldl ssiqetvpvp vsswsfqqdd
      301 slkpitmsrw emivpayqdd nmdhsrekgd mdvaedivfr plfkyrqqmq qrskyydesn
      361 rryggysnny prrayfyrpr ydny
//
LOCUS       XP_034176309             344 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602416 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176309
VERSION     XP_034176309.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320418.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..344
                     /product="uncharacterized protein LOC117602416 isoform X3"
                     /calculated_mol_wt=38196
     CDS             1..344
                     /gene="LOC117602416"
                     /coded_by="XM_034320418.2:127..1161"
                     /db_xref="GeneID:117602416"
ORIGIN      
        1 mnvlqylrii ttfqlilcli lilnggistc kplssedveg llpptprdkn etiaavvqdp
       61 vvqdavqkva nngslnglvv kknvfimpas npnmilskre hilvvptenl edvrknitet
      121 kqteaamets spesemsies dadeympvei esgpqqnidl qsspvenpgq smltneqlks
      181 ktefledais hdiplpedqp kkvpvpiiav idpskaekgk vnspivailp nqldndalkn
      241 qvqflndnnd viqkkaqdyi dqssftivpd yrksstpsei tldlssiqet vpvpvsswsf
      301 qqddslkpit msrwemivpa yqddnmdhsr ekdhdttiir need
//
LOCUS       XP_034176323             369 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602429 [Osmia lignaria lignaria].
ACCESSION   XP_034176323
VERSION     XP_034176323.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320432.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176323.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="uncharacterized protein LOC117602429"
                     /calculated_mol_wt=39891
     CDS             1..369
                     /gene="LOC117602429"
                     /coded_by="XM_034320432.2:2364..3473"
                     /db_xref="GeneID:117602429"
ORIGIN      
        1 myaaaggasi anrrkqkrlq lnkrvaegti psrlkpipaa ssqhhqhqhq hhhhhhhhhp
       61 skfarphvvp psstqqppqp pssafhpsid rrrhvekcqq vapvspiqfp isppplsles
      121 pssvtcatks nnfpfpppsi ldlrvspssl elqcgggpgk aawqgcsrps plplspmsph
      181 gcrgdgyktk qceahrrwmk rnrirdasyc ygssgedded dgssnanrrr gevsaavntv
      241 lyaglgttal glvisfvgtg ekgflspelr lvgpsllcag llcclfrvll clclcrcgqc
      301 rwrfchvasd kkekvrktda rpagtlptas llspaqqqrq qqsampssga assstkahel
      361 llspaqlpe
//
LOCUS       XP_034176332             369 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602429 [Osmia lignaria lignaria].
ACCESSION   XP_034176332
VERSION     XP_034176332.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320441.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176332.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="uncharacterized protein LOC117602429"
                     /calculated_mol_wt=39891
     CDS             1..369
                     /gene="LOC117602429"
                     /coded_by="XM_034320441.2:670..1779"
                     /db_xref="GeneID:117602429"
ORIGIN      
        1 myaaaggasi anrrkqkrlq lnkrvaegti psrlkpipaa ssqhhqhqhq hhhhhhhhhp
       61 skfarphvvp psstqqppqp pssafhpsid rrrhvekcqq vapvspiqfp isppplsles
      121 pssvtcatks nnfpfpppsi ldlrvspssl elqcgggpgk aawqgcsrps plplspmsph
      181 gcrgdgyktk qceahrrwmk rnrirdasyc ygssgedded dgssnanrrr gevsaavntv
      241 lyaglgttal glvisfvgtg ekgflspelr lvgpsllcag llcclfrvll clclcrcgqc
      301 rwrfchvasd kkekvrktda rpagtlptas llspaqqqrq qqsampssga assstkahel
      361 llspaqlpe
//
LOCUS       XP_034176342             369 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602429 [Osmia lignaria lignaria].
ACCESSION   XP_034176342
VERSION     XP_034176342.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320451.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176342.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="uncharacterized protein LOC117602429"
                     /calculated_mol_wt=39891
     CDS             1..369
                     /gene="LOC117602429"
                     /coded_by="XM_034320451.2:1056..2165"
                     /db_xref="GeneID:117602429"
ORIGIN      
        1 myaaaggasi anrrkqkrlq lnkrvaegti psrlkpipaa ssqhhqhqhq hhhhhhhhhp
       61 skfarphvvp psstqqppqp pssafhpsid rrrhvekcqq vapvspiqfp isppplsles
      121 pssvtcatks nnfpfpppsi ldlrvspssl elqcgggpgk aawqgcsrps plplspmsph
      181 gcrgdgyktk qceahrrwmk rnrirdasyc ygssgedded dgssnanrrr gevsaavntv
      241 lyaglgttal glvisfvgtg ekgflspelr lvgpsllcag llcclfrvll clclcrcgqc
      301 rwrfchvasd kkekvrktda rpagtlptas llspaqqqrq qqsampssga assstkahel
      361 llspaqlpe
//
LOCUS       XP_034176366             368 aa            linear   INV 03-SEP-2025
DEFINITION  actin-related protein 2/3 complex, subunit 1A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034176366
VERSION     XP_034176366.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320475.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..368
                     /product="actin-related protein 2/3 complex, subunit 1A
                     isoform X1"
                     /calculated_mol_wt=40701
     Region          <5..267
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          12..51
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          57..95
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          101..140
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          148..201
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          210..247
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..368
                     /gene="Arpc1"
                     /coded_by="XM_034320475.2:466..1572"
                     /db_xref="GeneID:117602442"
ORIGIN      
        1 mtevhslgvd aischawnkd rkevaicpnn neiqvhkhts ggwkllqnlq ehdmhvmgid
       61 wapntnrivt csadknayvw tqeqdgkwnp awvllrinra atcvkwsple nkfavgsggr
      121 viavcyfvse nnwwqckhik rplrstvttv dwhpdnkvlv agstdykvrv fsafisdmed
      181 apgngpwghs ntlgtllaef qntpngggwi hcvafspcgn kicwvahnss iciadatkgn
      241 avmrlytehl pflscvwmgs nsivaaghsc mpmlysvddn gqlyfvskld ntqkkeaagl
      301 samrkfqsld rqarsgtndn aldsihqnti ncvrkvsdne fstsgldgql vvwdlkllen
      361 siaglkia
//
LOCUS       XP_034176376             340 aa            linear   INV 03-SEP-2025
DEFINITION  actin-related protein 2/3 complex, subunit 1A isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034176376
VERSION     XP_034176376.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="actin-related protein 2/3 complex, subunit 1A
                     isoform X2"
                     /calculated_mol_wt=37745
     Region          <5..>173
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          12..51
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          57..95
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          101..140
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          247..295
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..340
                     /gene="Arpc1"
                     /coded_by="XM_034320485.2:469..1491"
                     /db_xref="GeneID:117602442"
ORIGIN      
        1 mtevhslgvd aischawnkd rkevaicpnn neiqvhkhts ggwkllqnlq ehdmhvmgid
       61 wapntnrivt csadknayvw tqeqdgkwnp awvllrinra atcvkwsple nkfavgsggr
      121 viavcyfvse nnwwqckhik rplrstvttv dwhpdnkvlv agstdykvrv fsafisdmed
      181 apgngpwghs ntlgtllaef qntpngdatk gnavmrlyte hlpflscvwm gsnsivaagh
      241 scmpmlysvd dngqlyfvsk ldntqkkeaa glsamrkfqs ldrqarsgtn dnaldsihqn
      301 tincvrkvsd nefstsgldg qlvvwdlkll ensiaglkia
//
LOCUS       XP_034176389             339 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome biogenesis regulatory protein homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034176389
VERSION     XP_034176389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="ribosome biogenesis regulatory protein homolog"
                     /calculated_mol_wt=38547
     Region          28..192
                     /region_name="RRS1"
                     /note="Ribosome biogenesis regulatory protein (RRS1);
                     pfam04939"
                     /db_xref="CDD:461494"
     CDS             1..339
                     /gene="LOC117602449"
                     /coded_by="XM_034320498.2:61..1080"
                     /db_xref="GeneID:117602449"
ORIGIN      
        1 mdivqsilen daqekdprks tvvnkdvdve tdagtllvld sntldlkalk aqqepylrsl
       61 trdnvqllin kiwelpteri devivaklpk phfvlprsrq lpkpkpltkw qkfakekgir
      121 tkkkgksklq wddelqkwip tfgykrktal eqkewlvevn denkamedpf atakaakeer
      181 qsknelqrlr niakakniki pkvglptkeh fpeakqlsqa itiartstas vgkfqdrlpk
      241 ekdakgiakq vpgmkrkaen vplnlndekk rntdlaenil ksntkifred fsapktkvne
      301 tktktkkgkk gvkskgakkp kggkgkrdmr lkvggrkrr
//
LOCUS       XP_076546094             317 aa            linear   INV 03-SEP-2025
DEFINITION  abasic site processing protein HMCES-like [Osmia lignaria
            lignaria].
ACCESSION   XP_076546094
VERSION     XP_076546094.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689979.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="abasic site processing protein HMCES-like"
                     /calculated_mol_wt=35523
     Region          1..259
                     /region_name="SRAP"
                     /note="SOS response associated peptidase (SRAP);
                     pfam02586"
                     /db_xref="CDD:460605"
     CDS             1..317
                     /gene="LOC143305642"
                     /coded_by="XM_076689979.1:135..1088"
                     /db_xref="GeneID:143305642"
ORIGIN      
        1 mcgrtacsln pdtlsracgy kdatgkqpit swakndvqyt pscnigpkdv lpcliagshv
       61 dlederalca mfwsmippwh egdyrkfnls thnarlenik tsklygpplr kgqrcvvvce
      121 gyyewkagkt kkdpkqpyyi yatqkegvra ddpttwkdew seelgwqgfk mlkmaglfsv
      181 fktgdgktih sctvittnsn dvmsglhdrv pvfltteedi qtwlnedlsa aeavdklnkl
      241 tlspgdlswh tvstlvnnvl cksdncrret kpleekktnp sgfmaswlqk gstesdkrks
      301 teteetdevp skstkkt
//
LOCUS       XP_034176457             224 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602478 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176457
VERSION     XP_034176457.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320566.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..224
                     /product="uncharacterized protein LOC117602478 isoform X1"
                     /calculated_mol_wt=24293
     CDS             1..224
                     /gene="LOC117602478"
                     /coded_by="XM_034320566.2:70..744"
                     /db_xref="GeneID:117602478"
ORIGIN      
        1 mvfanqseve gliackmkni lavlmlvaia kgspvwgdyq vgqwnkeyqp pgqwysssgy
       61 spplsgpsfv ppgqgflppg hsrtppghqh ippghmylkp cqmleqvlnl qanqpsnsld
      121 kscnvgwicr npgtddmtit tidgtqiiig rqeqpnnwpn qngiqsnnkn pmsddptqgr
      181 adnanlpnen stpsvnnpti pqtnnessrr eggeglidvr igdk
//
LOCUS       XP_034176474             208 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602478 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176474
VERSION     XP_034176474.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320583.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="uncharacterized protein LOC117602478 isoform X2"
                     /calculated_mol_wt=22572
     CDS             1..208
                     /gene="LOC117602478"
                     /coded_by="XM_034320583.2:144..770"
                     /db_xref="GeneID:117602478"
ORIGIN      
        1 mknilavlml vaiakgspvw gdyqvgqwnk eyqppgqwys ssgyspplsg psfvppgqgf
       61 lppghsrtpp ghqhippghm ylkpcqmleq vlnlqanqps nsldkscnvg wicrnpgtdd
      121 mtittidgtq iiigrqeqpn nwpnqngiqs nnknpmsddp tqgradnanl pnenstpsvn
      181 nptipqtnne ssrreggegl idvrigdk
//
LOCUS       XP_034176483             208 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602478 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176483
VERSION     XP_034176483.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320592.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="uncharacterized protein LOC117602478 isoform X2"
                     /calculated_mol_wt=22572
     CDS             1..208
                     /gene="LOC117602478"
                     /coded_by="XM_034320592.2:143..769"
                     /db_xref="GeneID:117602478"
ORIGIN      
        1 mknilavlml vaiakgspvw gdyqvgqwnk eyqppgqwys ssgyspplsg psfvppgqgf
       61 lppghsrtpp ghqhippghm ylkpcqmleq vlnlqanqps nsldkscnvg wicrnpgtdd
      121 mtittidgtq iiigrqeqpn nwpnqngiqs nnknpmsddp tqgradnanl pnenstpsvn
      181 nptipqtnne ssrreggegl idvrigdk
//
LOCUS       XP_034176495             220 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602494 [Osmia lignaria lignaria].
ACCESSION   XP_034176495
VERSION     XP_034176495.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320604.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..220
                     /product="uncharacterized protein LOC117602494"
                     /calculated_mol_wt=25534
     CDS             1..220
                     /gene="LOC117602494"
                     /coded_by="XM_034320604.2:126..788"
                     /db_xref="GeneID:117602494"
ORIGIN      
        1 mwtvlvlicf issfdigatk dqlhpqsyie hpdstettlr kledpsvrlp rrfrensrtr
       61 rlhienqtgf dgkvekihin seedlpgira yglqkknlmn ngyiermiek evkedlndas
      121 rvtrsveayg kskdhletng kieriqspvs pratsepgnr vsrsiatesk gepddleaqd
      181 akifrplfvy rqqqakrqhr aknrflqmnq nsfhnrrpff
//
LOCUS       XP_034176510             220 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear factor Y-box B [Osmia lignaria lignaria].
ACCESSION   XP_034176510
VERSION     XP_034176510.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320619.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..220
                     /product="nuclear factor Y-box B"
                     /calculated_mol_wt=23246
     Region          67..157
                     /region_name="HFD_NFYB"
                     /note="histone-fold domain found in nuclear transcription
                     factor Y subunit beta (NF-YB) and similar proteins;
                     cd22907"
                     /db_xref="CDD:467032"
     Site            order(67,69..70,73,99,102..103,107,110..111,114..115,118,
                     138..140,142)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:467032"
     Site            order(70..75,78,82..83,86..88,91..94,97,100..101,104..109,
                     111..114,117,126..130,133,137,141..142,145,149,152,
                     156..157)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467032"
     Site            order(70,75..78,81,84,92..95,98,126..127)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467032"
     CDS             1..220
                     /gene="Nf-YB"
                     /coded_by="XM_034320619.2:118..780"
                     /db_xref="GeneID:117602502"
ORIGIN      
        1 mensgesgdd ggplsttafl ggsgvsasyi svqsddmedd pentddsnhg tsdplqgagg
       61 gsgggplreq drflpianva kimkraipea gkiakdarec vqecvsefis fitseasdrc
      121 hmekrkting edilfamttl gfdnyveplk vylqkyreat kgdnppgsgt pagngkvepq
      181 gtmyedqlfa iaatassatt sdtpviysyt stdqmqfqls
//
LOCUS       XP_076546095             209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305643 [Osmia lignaria lignaria].
ACCESSION   XP_076546095
VERSION     XP_076546095.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689980.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="uncharacterized protein LOC143305643"
                     /calculated_mol_wt=22989
     CDS             1..209
                     /gene="LOC143305643"
                     /coded_by="XM_076689980.1:108..737"
                     /db_xref="GeneID:143305643"
ORIGIN      
        1 mwtlrtacll itltmvasqk yfhrpvndls tgerflsdlh vvtrevqesa llqkktmvda
       61 mtavskatfg vlgdlkrsns givstlvdie makleaikaa krvllnmlas qiaslmeeis
      121 nediielvqs ppfnhlvdll lpllsgsskm mdtvninnir aqqnpetaat reyssrdtts
      181 smqrpsstts fplsrespsl qsvknfskl
//
LOCUS       XP_034176525             204 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase III subunit H [Osmia lignaria lignaria].
ACCESSION   XP_034176525
VERSION     XP_034176525.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320634.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..204
                     /product="RNA polymerase III subunit H"
                     /calculated_mol_wt=23058
     Region          2..81
                     /region_name="RNAP_III_Rpc25_N"
                     /note="Rpc25, N-terminal ribonucleoprotein (RNP) domain.
                     Rpc25 is a subunit of eukaryotic RNA polymerase (RNAP) III
                     and is homologous to Rpa43 of eukaryotic RNAP I, Rpb7 of
                     eukaryotic RNAP II, and RpoE of archaeal RNAP. Rpc25 has
                     two domains, an N-terminal RNP...; cd04330"
                     /db_xref="CDD:239822"
     Site            order(2..6,8,28,31..32,46..49,73,78,80)
                     /site_type="other"
                     /note="Rpc17 interaction site [polypeptide binding]"
                     /db_xref="CDD:239822"
     Region          83..201
                     /region_name="RNA_pol_Rbc25"
                     /note="RNA polymerase III subunit Rpc25; pfam08292"
                     /db_xref="CDD:400543"
     CDS             1..204
                     /gene="Polr3H"
                     /coded_by="XM_034320634.2:190..804"
                     /db_xref="GeneID:117602504"
ORIGIN      
        1 mfvlaelkdt vrippwefkr klndavtdel nrklankvyl nvglcialhd itkieesyif
       61 pgdgashtkv ifrfivfrpf meeiligkir scsvdgvhvt lgffedivip phklqhpsrf
      121 dqmeqawvwe ydtgdgekhd lfmdageiir frvvsenfte alptgpnmsg egvekseskn
      181 tspytlggai depglglltw wens
//
LOCUS       XP_034176535             204 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase III subunit H [Osmia lignaria lignaria].
ACCESSION   XP_034176535
VERSION     XP_034176535.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320644.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..204
                     /product="RNA polymerase III subunit H"
                     /calculated_mol_wt=23058
     Region          2..81
                     /region_name="RNAP_III_Rpc25_N"
                     /note="Rpc25, N-terminal ribonucleoprotein (RNP) domain.
                     Rpc25 is a subunit of eukaryotic RNA polymerase (RNAP) III
                     and is homologous to Rpa43 of eukaryotic RNAP I, Rpb7 of
                     eukaryotic RNAP II, and RpoE of archaeal RNAP. Rpc25 has
                     two domains, an N-terminal RNP...; cd04330"
                     /db_xref="CDD:239822"
     Site            order(2..6,8,28,31..32,46..49,73,78,80)
                     /site_type="other"
                     /note="Rpc17 interaction site [polypeptide binding]"
                     /db_xref="CDD:239822"
     Region          83..201
                     /region_name="RNA_pol_Rbc25"
                     /note="RNA polymerase III subunit Rpc25; pfam08292"
                     /db_xref="CDD:400543"
     CDS             1..204
                     /gene="Polr3H"
                     /coded_by="XM_034320644.2:191..805"
                     /db_xref="GeneID:117602504"
ORIGIN      
        1 mfvlaelkdt vrippwefkr klndavtdel nrklankvyl nvglcialhd itkieesyif
       61 pgdgashtkv ifrfivfrpf meeiligkir scsvdgvhvt lgffedivip phklqhpsrf
      121 dqmeqawvwe ydtgdgekhd lfmdageiir frvvsenfte alptgpnmsg egvekseskn
      181 tspytlggai depglglltw wens
//
LOCUS       XP_076546097             144 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305644 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546097
VERSION     XP_076546097.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689982.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..144
                     /product="uncharacterized protein LOC143305644 isoform X1"
                     /calculated_mol_wt=16335
     CDS             1..144
                     /gene="LOC143305644"
                     /coded_by="XM_076689982.1:45..479"
                     /db_xref="GeneID:143305644"
ORIGIN      
        1 mvqpltiksq adssnfnelv emkenekiqn rqksrrksvv vhsislivlh vldtlllmvl
       61 lpfmiwqwvs sssrfklvlk avievtlell mwvifagvsi lmttafvldd ayfrnndqek
      121 ltqliktqnv stsgqdngar ilet
//
LOCUS       XP_076546098             139 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305644 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546098
VERSION     XP_076546098.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689983.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..139
                     /product="uncharacterized protein LOC143305644 isoform X2"
                     /calculated_mol_wt=15679
     CDS             1..139
                     /gene="LOC143305644"
                     /coded_by="XM_076689983.1:36..455"
                     /db_xref="GeneID:143305644"
ORIGIN      
        1 mvqpltiksq adssnfnelv emkenekiqn rksvvvhsis livlhvldtl llmvllpfmi
       61 wqwvssssrf klvlkaviev tlellmwvif agvsilmtta fvlddayfrn ndqekltqli
      121 ktqnvstsgq dngarilet
//
LOCUS       XP_034176598             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602535 [Osmia lignaria lignaria].
ACCESSION   XP_034176598
VERSION     XP_034176598.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320707.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117602535"
                     /calculated_mol_wt=13186
     CDS             1..116
                     /gene="LOC117602535"
                     /coded_by="XM_034320707.2:141..491"
                     /db_xref="GeneID:117602535"
ORIGIN      
        1 mtplaiaffl avfgssialp vnpdsklgpv vripilfekf ndesspalyt lllsklnepe
       61 niiddkliek reakeeeqdd letaagnyal rplfvyrqql ayrqrvrday rrrngf
//
LOCUS       XP_034175387             234 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602036 [Osmia lignaria lignaria].
ACCESSION   XP_034175387
VERSION     XP_034175387.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175387.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..234
                     /product="uncharacterized protein LOC117602036"
                     /calculated_mol_wt=24457
     Region          41..110
                     /region_name="Linker_histone"
                     /note="linker histone H1 and H5 family; pfam00538"
                     /db_xref="CDD:459846"
     Region          <201..234
                     /region_name="COG1754"
                     /note="Uncharacterized C-terminal domain of topoisomerase
                     IA [Function unknown]"
                     /db_xref="CDD:441360"
     CDS             1..234
                     /gene="LOC117602036"
                     /coded_by="XM_034319496.2:65..769"
                     /db_xref="GeneID:117602036"
ORIGIN      
        1 mmeeknssvn tatvenstan pspakksaks ktksqrskss hpptadmvna aikalqdrkg
       61 ssvqaikkyi attykvdgek mapfikrylk savtagavvq tkgkgasgsf klstsskgse
      121 skskskrlvk ravsapaekk aaekkkpasp kkaaapkktp tvkkptaaak kqpppkkaka
      181 atvkkaesve qkatpppknl skakkttkap aaktktpkpk takspkiara takk
//
LOCUS       XP_034195020             136 aa            linear   INV 03-SEP-2025
DEFINITION  histone H3 [Osmia lignaria lignaria].
ACCESSION   XP_034195020
VERSION     XP_034195020.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339129.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="histone H3"
                     /calculated_mol_wt=15257
     Region          1..136
                     /region_name="PTZ00018"
                     /note="histone H3; Provisional"
                     /db_xref="CDD:185400"
     Site            order(40..48,50,64..67,70,73,84..87,117..119,121)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467036"
     Site            order(45,48,51..52,55..56,58,60,62..63,67..68,70..72,
                     74..75,77,79,82..86,88..89,92..93,96..98,100..102,
                     104..106,109,118..122,126,129)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     Site            order(49,52..53,56..57,59,95,99,102,109,113,118)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     Site            order(107,110..111,114..115,117,123..124,127..128,
                     130..132)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     CDS             1..136
                     /gene="LOC117611194"
                     /coded_by="XM_034339129.2:33..443"
                     /db_xref="GeneID:117611194"
ORIGIN      
        1 martkqtark stggkaprkq latkaarksa patggvkkph ryrpgtvalr eirryqkste
       61 llirklpfqr lvreiaqdfk tdlrfqssav malqeaseay lvglfedtnl caihakrvti
      121 mpkdiqlarr irgera
//
LOCUS       XP_076546100             124 aa            linear   INV 03-SEP-2025
DEFINITION  histone H2A [Osmia lignaria lignaria].
ACCESSION   XP_076546100
VERSION     XP_076546100.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689985.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="histone H2A"
                     /calculated_mol_wt=13189
     Region          16..124
                     /region_name="PTZ00017"
                     /note="histone H2A; Provisional"
                     /db_xref="CDD:185399"
     CDS             1..124
                     /gene="LOC117600337"
                     /coded_by="XM_076689985.1:38..412"
                     /db_xref="GeneID:117600337"
ORIGIN      
        1 msgrgkggka kgkaksrsnr aglqfpvgri hrllrkgnya ervgagapvy laavmeylaa
       61 evlelagnaa rdnkktriip rhlqlairnd eelnkllsgv tiaqggvlpn iqavllpkkt
      121 dkka
//
LOCUS       XP_034171974             123 aa            linear   INV 03-SEP-2025
DEFINITION  histone H2B [Osmia lignaria lignaria].
ACCESSION   XP_034171974
VERSION     XP_034171974.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316083.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="histone H2B"
                     /calculated_mol_wt=13563
     Region          33..120
                     /region_name="HFD_H2B"
                     /note="histone-fold domain found in histone H2B and
                     similar proteins; cd22910"
                     /db_xref="CDD:467035"
     Site            order(33,35,37..39,41..43,46..53,55..56,59..60,63..64,
                     66..68,70..72,76,86..87,89,92..93,96,99..101,104,107..109,
                     111..112,115..116,119..120)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467035"
     Site            order(36..38,40..41,44..46,51..53,57,93,97,106..107,
                     109..110,113..114,116..117,120)
                     /site_type="other"
                     /note="Hif1 binding site [polypeptide binding]"
                     /db_xref="CDD:467035"
     Site            order(59,62..63,65..66,69,74,77,80..82,90..91,94,98..99)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467035"
     CDS             1..123
                     /gene="LOC117600498"
                     /coded_by="XM_034316083.2:47..418"
                     /db_xref="GeneID:117600498"
ORIGIN      
        1 mppkasgkav kkagkaqkni sktdkkkkrr rkesyaiyiy kvlkqvhpdt gisskamsim
       61 nsfvndvfer iaaeasrlah ynkrstitsr eiqtavrlll pgelakhavs egtkavtkyt
      121 ssk
//
LOCUS       XP_034172089             103 aa            linear   INV 03-SEP-2025
DEFINITION  histone H4 [Osmia lignaria lignaria].
ACCESSION   XP_034172089
VERSION     XP_034172089.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..103
                     /product="histone H4"
                     /calculated_mol_wt=11250
     Region          1..103
                     /region_name="PLN00035"
                     /note="histone H4; Provisional"
                     /db_xref="CDD:177669"
     CDS             1..103
                     /gene="LOC117600557"
                     /coded_by="XM_034316198.2:65..376"
                     /db_xref="GeneID:117600557"
ORIGIN      
        1 mtgrgkggkg lgkggakrhr kvlrdniqgi tkpairrlar rggvkrisgl iyeetrgvlk
       61 vflenvirda vtytehakrk tvtamdvvya lkrqgrtlyg fgg
//
LOCUS       XP_034184792             671 aa            linear   INV 03-SEP-2025
DEFINITION  calpain C [Osmia lignaria lignaria].
ACCESSION   XP_034184792
VERSION     XP_034184792.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328901.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..671
                     /product="calpain C"
                     /calculated_mol_wt=76236
     Region          23..329
                     /region_name="Peptidase_C2"
                     /note="Calpain family cysteine protease; pfam00648"
                     /db_xref="CDD:459889"
     Region          345..481
                     /region_name="Calpain_III"
                     /note="Calpain, subdomain III. Calpains are
                     calcium-activated cytoplasmic cysteine proteinases,
                     participate in cytoskeletal remodeling processes, cell
                     differentiation, apoptosis and signal transduction.
                     Catalytic domain and the two calmodulin-like domains
                     are...; cd00214"
                     /db_xref="CDD:238132"
     Region          505..670
                     /region_name="EFh_PEF_CalpC"
                     /note="Penta-EF hand, calcium binding motifs, found in
                     Drosophila melanogaster calpain-C (CalpC) and similar
                     proteins; cd16197"
                     /db_xref="CDD:320072"
     Region          505..533
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320072"
     Site            order(517,519,524,540,555,557,559,566,585,587,589,596,628,
                     630..631)
                     /site_type="other"
                     /note="putative Ca binding site [ion binding]"
                     /db_xref="CDD:320072"
     Region          546..575
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320072"
     Region          576..606
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320072"
     Region          612..640
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320072"
     Region          641..670
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320072"
     CDS             1..671
                     /gene="CalpC"
                     /coded_by="XM_034328901.2:501..2516"
                     /db_xref="GeneID:117606452"
ORIGIN      
        1 mvndvmseye rikrsclkrg elwedpefpa tqtsvfyhqt ppfqfqwkrp kelcnrpifv
       61 sdtpaqfdvi pgkmgdkwlv sclgvlhlsk glfyrvvpad qgfgnigepp gsptaeyagv
      121 frfrlwwcga wvevlvddrl ptihgrlafv qsrhsdqfwp allekayakl hgsyealkyg
      181 tlldglsdlt ggitesiair qdptacgrvl aklldmtsli tctvnnnqqq irasteklan
      241 giqmginyrl yaiervetfn geavqlvklr nplgpgreyv gawargglew devppmerer
      301 lavrnmaege fwisysdfvk tfthlevvhl daetsrdeps lhskhtwqmk lyqgswrrgv
      361 taggcrnnee tfhinpqlhl ilsemeeviv slyqhsimei kvigftaytl pknnaesink
      421 qffkknkslm nsqytnsrqv shrcqldqgg yllvpttfep tqetsftlrv ysskplklkl
      481 ldtpptlvks aiikappleg kgfsqyeavf lqladehrtv nafelqelle aclpndyiks
      541 cacmevcrqv vltmdssgsg rlkfndfkdl mcslkywqaa fknhtkektg ilkaerlrda
      601 llevgfqlnt dvlsililry mrkdgtlrfg dfvsailhls dafgifeskd plqngtikls
      661 ltewlrsslm c
//
LOCUS       XP_034173877             232 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine/tyrosine-interacting protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034173877
VERSION     XP_034173877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..232
                     /product="serine/threonine/tyrosine-interacting protein
                     isoform X1"
                     /calculated_mol_wt=26177
     Region          44..194
                     /region_name="DSP_STYX"
                     /note="dual specificity phosphatase-like domain of
                     serine/threonine/tyrosine-interacting protein; cd14522"
                     /db_xref="CDD:350372"
     CDS             1..232
                     /gene="LOC117601338"
                     /coded_by="XM_034317986.2:137..835"
                     /db_xref="GeneID:117601338"
ORIGIN      
        1 mlsldsngtq qdevhaetts dngsddypri pssmdgpkew tytmrrhmqe vvpglylgpy
       61 saasrsklqt lldhgithiv cvrqdieanf ikpnfpdkfk ylvlniadta teniiqhfhk
      121 vktfidegln sggqvlvhgn agisrsaalv layvmetygl sqtrayaivq qrrfcinpng
      181 gfmaqlreye piyqaqktlk ngqqsslrqr skrtihqmdt driedkydtm ds
//
LOCUS       XP_034173886             229 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine/tyrosine-interacting protein isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034173886
VERSION     XP_034173886.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317995.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..229
                     /product="serine/threonine/tyrosine-interacting protein
                     isoform X2"
                     /calculated_mol_wt=25869
     Region          41..191
                     /region_name="DSP_STYX"
                     /note="dual specificity phosphatase-like domain of
                     serine/threonine/tyrosine-interacting protein; cd14522"
                     /db_xref="CDD:350372"
     CDS             1..229
                     /gene="LOC117601338"
                     /coded_by="XM_034317995.2:136..825"
                     /db_xref="GeneID:117601338"
ORIGIN      
        1 mlsldsngtq qdeettsdng sddypripss mdgpkewtyt mrrhmqevvp glylgpysaa
       61 srsklqtlld hgithivcvr qdieanfikp nfpdkfkylv lniadtaten iiqhfhkvkt
      121 fideglnsgg qvlvhgnagi srsaalvlay vmetyglsqt rayaivqqrr fcinpnggfm
      181 aqlreyepiy qaqktlkngq qsslrqrskr tihqmdtdri edkydtmds
//
LOCUS       XP_034173892             221 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine/tyrosine-interacting protein isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034173892
VERSION     XP_034173892.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318001.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="serine/threonine/tyrosine-interacting protein
                     isoform X3"
                     /calculated_mol_wt=24903
     Region          44..183
                     /region_name="DSP_STYX"
                     /note="dual specificity phosphatase-like domain of
                     serine/threonine/tyrosine-interacting protein; cd14522"
                     /db_xref="CDD:350372"
     CDS             1..221
                     /gene="LOC117601338"
                     /coded_by="XM_034318001.2:138..803"
                     /db_xref="GeneID:117601338"
ORIGIN      
        1 mlsldsngtq qdevhaetts dngsddypri pssmdgpkew tytmrrhmqe vvpglylgpy
       61 saasrsklqt lldhgithiv cvrqdieanf ikpnfpdkfk ylvlniadta teniiqhfhk
      121 vktfidegln sggqvlvhgn agisrsaalv larayaivqq rrfcinpngg fmaqlreyep
      181 iyqaqktlkn gqqsslrqrs krtihqmdtd riedkydtmd s
//
LOCUS       XP_034173145             153 aa            linear   INV 03-SEP-2025
DEFINITION  NPC intracellular cholesterol transporter 2 homolog a [Osmia
            lignaria lignaria].
ACCESSION   XP_034173145
VERSION     XP_034173145.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317254.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="NPC intracellular cholesterol transporter 2
                     homolog a"
                     /calculated_mol_wt=16433
     Region          27..150
                     /region_name="Npc2_like"
                     /note="Niemann-Pick type C2 (Npc2) is a lysosomal protein
                     in which a mutation in the gene causes a rare form of
                     Niemann-Pick type C disease, an autosomal recessive lipid
                     storage disorder characterized by accumulation of
                     low-density lipoprotein-derived...; cd00916"
                     /db_xref="CDD:238458"
     Site            order(87,120,124)
                     /site_type="other"
                     /note="putative cholesterol/lipid binding site [chemical
                     binding]"
                     /db_xref="CDD:238458"
     CDS             1..153
                     /gene="LOC117601031"
                     /coded_by="XM_034317254.2:256..717"
                     /db_xref="GeneID:117601031"
ORIGIN      
        1 mcrtiaailf lcflsstpic rafdigdcgs kvgkftsvsl gcdmtkpace lihntnetiv
       61 ldfvvdkdvk kvtavvhgiv mdvpvpfpls nanacdtads gltcplakgg sfqykntlpi
      121 hksypklsvt vkwelkdenn enivcvlipa kik
//
LOCUS       XP_034171183             783 aa            linear   INV 03-SEP-2025
DEFINITION  protein KRI1 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034171183
VERSION     XP_034171183.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315292.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..783
                     /product="protein KRI1 homolog"
                     /calculated_mol_wt=93163
     Region          347..429
                     /region_name="Kri1"
                     /note="KRI1-like family; pfam05178"
                     /db_xref="CDD:461569"
     Region          498..585
                     /region_name="Kri1_C"
                     /note="KRI1-like family C-terminal; pfam12936"
                     /db_xref="CDD:463756"
     CDS             1..783
                     /gene="LOC117600204"
                     /coded_by="XM_034315292.2:204..2555"
                     /db_xref="GeneID:117600204"
ORIGIN      
        1 mptlfngdks dsdeelkint dyaknynnwr qkeefnklkt kygnvsgsgt sdedsdtess
       61 seeeeeenel tqqfdkdfyk tlaylkqkdp kiynqdvtff ddtnkaqely sekkkyktkk
      121 ekavflrdye rniiveregk fsdsedestl kmneehsski tyaqeqrqlk esfknalhdv
      181 eeeedtdllk pktksesekq keeldykewl kgqetaiden eqkvlkplrd fwsnpnldsn
      241 ekflrdyiln nkyldneytg pnvddehivh dsdenlsede kniveqeefe hkynfrfeep
      301 dqefikrypr tlenslrrkd trraqkraev kkrkeeeklr kkeelkqlka lkrkeieeki
      361 eklkeitgnd diqfndidle gdfdptehdr kmtqlfnddy yaateenikp efpdideele
      421 ientwddydp ntdyyneeey egphcedadf nmdadydpsk svkeeeegtk krkrrrkskf
      481 aeliakekpk fdpkvyqsye eyfdkyysld yedmigdipc rfkyrkvvpn dyglsveeil
      541 vaddkelnkw cslkkalqhk pdhaelndvr mykekarnea lkkkvlrsly tnpedeeetd
      601 kkeiidnqni aestetkkrr rkkkkkkeev qnnssitetv svndkesses kkkvkvnanq
      661 ktskeskess ktekrkqpee qseqpskkkr kinnskkstp ivedkpqkeq gqepdesnvk
      721 hpkkekmkkk mikkktlgki kkkgaknnni ngditslnpe rlkmyginpk klknklkygk
      781 qqq
//
LOCUS       XP_034171194             441 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600212 [Osmia lignaria lignaria].
ACCESSION   XP_034171194
VERSION     XP_034171194.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315303.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171194.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="uncharacterized protein LOC117600212"
                     /calculated_mol_wt=50656
     Region          15..402
                     /region_name="7tm_7"
                     /note="7tm Chemosensory receptor; pfam08395"
                     /db_xref="CDD:462463"
     CDS             1..441
                     /gene="LOC117600212"
                     /coded_by="XM_034315303.2:130..1455"
                     /db_xref="GeneID:117600212"
ORIGIN      
        1 myepktidra isplllmqli tglgvfsdsn nrvikffefl ysgcymvify ytfqygiely
       61 dnlyflnvfm lnrisikyth manaiimgsl ialgrlnakn vknvialfnk ydqsaeemgl
      121 preyrvayqy qlyavaisal filtsavtqy llylppmlql ykkaiiifvl hfplllysvc
      181 visfcfwirc lelkfqqlnd llrsleaakp dlpqekrilt mfydfnnkkf sslenedikr
      241 dkgdaltmrl vkqahlelik iagvvnstyg vqilllifsa ftylvtslht nyktlwlspd
      301 meqtlrlivl ptfaacfcgl eifavghacg ktstesastg dlfcklyqts tskefraeir
      361 dftlqmvqnp lmftacgfft vdhsfilsii gvvitylvil iqmedtttkt yrrllernlt
      421 tendapigcn dfnmnctiky n
//
LOCUS       XP_076546101            1245 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600701 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546101
VERSION     XP_076546101.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689986.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="uncharacterized protein LOC117600701 isoform X1"
                     /calculated_mol_wt=142628
     CDS             1..1245
                     /gene="LOC117600701"
                     /coded_by="XM_076689986.1:158..3895"
                     /db_xref="GeneID:117600701"
ORIGIN      
        1 mseenkhtse etesemnten qireqsksdt silnneseqp mledeemptf vsvsspsrrd
       61 dsdpsnvkqq cripveedhs rspcpsddqg ggsiyvlssg eesnhpynde eedeygdsde
      121 mdemdleneh ehehgmlede eeedeeddde rnplrmpned dedidteydq eyfdrvsddf
      181 ilnnrlkqhh kerslslqdl sisknnvhsp ynkkrhslni vrqnyfgfqq npvmysiihk
      241 kqmvpvkyqh veskvkqyir dikeqnkrsm ekrikeqefv enktyeeden ndteqvntpv
      301 inkvikdyaq ktikdlqree iegtnlvyni aqivknsknn ktdnkvienq fkhmrkdsey
      361 vniqnekraq ssddklekrn gsvdinvhqi eyqntldrni kfnnmnqspl vngheqtpaf
      421 inlrtvtyee ymhgitnseq kdeldeqkek ahetevynea emineqeidn sntscslkie
      481 svksirmsqd esgnlnfakv nltddivtes teiiqlktql sqkdaqfhhl kdayqktlae
      541 nikmkqelda lkqslakyen enkaretkva svqtetveks vcnqettttg einkkvstss
      601 vastasssdq waesayspav sikppdltsi lnsddsivlt natprkiahp lsrtfitssr
      661 ilqtlsnitq gktktesplv qntkkglnen ltdqlvnshy sspthtsssk krkatemlgs
      721 stftqpfkip htslesdrks nintanvefk fveehakska eqeqnasink nidgststds
      781 kteetvdndn gvkcfiyaed ehsknksfli qaeeplkdgi neknrvqecg pyllgnlevr
      841 mseingtisv wgkevnrddi ndneddtevs itpsekktcn cwqstpemrf nesplvcstn
      901 kklripsrfn rsnsnssqyr rnlssnvmgt sslgddlhdk ihtdhsfnln ncvlscekyd
      961 spkyneewpi ckgasssqek lhsccihppe lvneerrcdl hhekqkydls pkhlknklyk
     1021 girrnsyknt fipdsknrln dnvtntniet nevtskcrtc qrsfqnvvan pthskfsniv
     1081 rnfsysckss ldnisnhens ekhtcshsvs nddeedplip frrsnetpet rqrrlsgkkv
     1141 rgilmdllrg cgdcrsssst nlikneqqtk eapytvngpp qikitspsmg ptssgstqcn
     1201 ercchayalr iesqleefrt emervrsrsd aildmlnmlh svdmn
//
LOCUS       XP_034172340            1245 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600701 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172340
VERSION     XP_034172340.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316449.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172340.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="uncharacterized protein LOC117600701 isoform X1"
                     /calculated_mol_wt=142628
     CDS             1..1245
                     /gene="LOC117600701"
                     /coded_by="XM_034316449.2:162..3899"
                     /db_xref="GeneID:117600701"
ORIGIN      
        1 mseenkhtse etesemnten qireqsksdt silnneseqp mledeemptf vsvsspsrrd
       61 dsdpsnvkqq cripveedhs rspcpsddqg ggsiyvlssg eesnhpynde eedeygdsde
      121 mdemdleneh ehehgmlede eeedeeddde rnplrmpned dedidteydq eyfdrvsddf
      181 ilnnrlkqhh kerslslqdl sisknnvhsp ynkkrhslni vrqnyfgfqq npvmysiihk
      241 kqmvpvkyqh veskvkqyir dikeqnkrsm ekrikeqefv enktyeeden ndteqvntpv
      301 inkvikdyaq ktikdlqree iegtnlvyni aqivknsknn ktdnkvienq fkhmrkdsey
      361 vniqnekraq ssddklekrn gsvdinvhqi eyqntldrni kfnnmnqspl vngheqtpaf
      421 inlrtvtyee ymhgitnseq kdeldeqkek ahetevynea emineqeidn sntscslkie
      481 svksirmsqd esgnlnfakv nltddivtes teiiqlktql sqkdaqfhhl kdayqktlae
      541 nikmkqelda lkqslakyen enkaretkva svqtetveks vcnqettttg einkkvstss
      601 vastasssdq waesayspav sikppdltsi lnsddsivlt natprkiahp lsrtfitssr
      661 ilqtlsnitq gktktesplv qntkkglnen ltdqlvnshy sspthtsssk krkatemlgs
      721 stftqpfkip htslesdrks nintanvefk fveehakska eqeqnasink nidgststds
      781 kteetvdndn gvkcfiyaed ehsknksfli qaeeplkdgi neknrvqecg pyllgnlevr
      841 mseingtisv wgkevnrddi ndneddtevs itpsekktcn cwqstpemrf nesplvcstn
      901 kklripsrfn rsnsnssqyr rnlssnvmgt sslgddlhdk ihtdhsfnln ncvlscekyd
      961 spkyneewpi ckgasssqek lhsccihppe lvneerrcdl hhekqkydls pkhlknklyk
     1021 girrnsyknt fipdsknrln dnvtntniet nevtskcrtc qrsfqnvvan pthskfsniv
     1081 rnfsysckss ldnisnhens ekhtcshsvs nddeedplip frrsnetpet rqrrlsgkkv
     1141 rgilmdllrg cgdcrsssst nlikneqqtk eapytvngpp qikitspsmg ptssgstqcn
     1201 ercchayalr iesqleefrt emervrsrsd aildmlnmlh svdmn
//
LOCUS       XP_034172358            1245 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600701 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172358
VERSION     XP_034172358.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316467.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172358.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="uncharacterized protein LOC117600701 isoform X1"
                     /calculated_mol_wt=142628
     CDS             1..1245
                     /gene="LOC117600701"
                     /coded_by="XM_034316467.2:158..3895"
                     /db_xref="GeneID:117600701"
ORIGIN      
        1 mseenkhtse etesemnten qireqsksdt silnneseqp mledeemptf vsvsspsrrd
       61 dsdpsnvkqq cripveedhs rspcpsddqg ggsiyvlssg eesnhpynde eedeygdsde
      121 mdemdleneh ehehgmlede eeedeeddde rnplrmpned dedidteydq eyfdrvsddf
      181 ilnnrlkqhh kerslslqdl sisknnvhsp ynkkrhslni vrqnyfgfqq npvmysiihk
      241 kqmvpvkyqh veskvkqyir dikeqnkrsm ekrikeqefv enktyeeden ndteqvntpv
      301 inkvikdyaq ktikdlqree iegtnlvyni aqivknsknn ktdnkvienq fkhmrkdsey
      361 vniqnekraq ssddklekrn gsvdinvhqi eyqntldrni kfnnmnqspl vngheqtpaf
      421 inlrtvtyee ymhgitnseq kdeldeqkek ahetevynea emineqeidn sntscslkie
      481 svksirmsqd esgnlnfakv nltddivtes teiiqlktql sqkdaqfhhl kdayqktlae
      541 nikmkqelda lkqslakyen enkaretkva svqtetveks vcnqettttg einkkvstss
      601 vastasssdq waesayspav sikppdltsi lnsddsivlt natprkiahp lsrtfitssr
      661 ilqtlsnitq gktktesplv qntkkglnen ltdqlvnshy sspthtsssk krkatemlgs
      721 stftqpfkip htslesdrks nintanvefk fveehakska eqeqnasink nidgststds
      781 kteetvdndn gvkcfiyaed ehsknksfli qaeeplkdgi neknrvqecg pyllgnlevr
      841 mseingtisv wgkevnrddi ndneddtevs itpsekktcn cwqstpemrf nesplvcstn
      901 kklripsrfn rsnsnssqyr rnlssnvmgt sslgddlhdk ihtdhsfnln ncvlscekyd
      961 spkyneewpi ckgasssqek lhsccihppe lvneerrcdl hhekqkydls pkhlknklyk
     1021 girrnsyknt fipdsknrln dnvtntniet nevtskcrtc qrsfqnvvan pthskfsniv
     1081 rnfsysckss ldnisnhens ekhtcshsvs nddeedplip frrsnetpet rqrrlsgkkv
     1141 rgilmdllrg cgdcrsssst nlikneqqtk eapytvngpp qikitspsmg ptssgstqcn
     1201 ercchayalr iesqleefrt emervrsrsd aildmlnmlh svdmn
//
LOCUS       XP_034172367            1245 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600701 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172367
VERSION     XP_034172367.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316476.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172367.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="uncharacterized protein LOC117600701 isoform X1"
                     /calculated_mol_wt=142628
     CDS             1..1245
                     /gene="LOC117600701"
                     /coded_by="XM_034316476.2:168..3905"
                     /db_xref="GeneID:117600701"
ORIGIN      
        1 mseenkhtse etesemnten qireqsksdt silnneseqp mledeemptf vsvsspsrrd
       61 dsdpsnvkqq cripveedhs rspcpsddqg ggsiyvlssg eesnhpynde eedeygdsde
      121 mdemdleneh ehehgmlede eeedeeddde rnplrmpned dedidteydq eyfdrvsddf
      181 ilnnrlkqhh kerslslqdl sisknnvhsp ynkkrhslni vrqnyfgfqq npvmysiihk
      241 kqmvpvkyqh veskvkqyir dikeqnkrsm ekrikeqefv enktyeeden ndteqvntpv
      301 inkvikdyaq ktikdlqree iegtnlvyni aqivknsknn ktdnkvienq fkhmrkdsey
      361 vniqnekraq ssddklekrn gsvdinvhqi eyqntldrni kfnnmnqspl vngheqtpaf
      421 inlrtvtyee ymhgitnseq kdeldeqkek ahetevynea emineqeidn sntscslkie
      481 svksirmsqd esgnlnfakv nltddivtes teiiqlktql sqkdaqfhhl kdayqktlae
      541 nikmkqelda lkqslakyen enkaretkva svqtetveks vcnqettttg einkkvstss
      601 vastasssdq waesayspav sikppdltsi lnsddsivlt natprkiahp lsrtfitssr
      661 ilqtlsnitq gktktesplv qntkkglnen ltdqlvnshy sspthtsssk krkatemlgs
      721 stftqpfkip htslesdrks nintanvefk fveehakska eqeqnasink nidgststds
      781 kteetvdndn gvkcfiyaed ehsknksfli qaeeplkdgi neknrvqecg pyllgnlevr
      841 mseingtisv wgkevnrddi ndneddtevs itpsekktcn cwqstpemrf nesplvcstn
      901 kklripsrfn rsnsnssqyr rnlssnvmgt sslgddlhdk ihtdhsfnln ncvlscekyd
      961 spkyneewpi ckgasssqek lhsccihppe lvneerrcdl hhekqkydls pkhlknklyk
     1021 girrnsyknt fipdsknrln dnvtntniet nevtskcrtc qrsfqnvvan pthskfsniv
     1081 rnfsysckss ldnisnhens ekhtcshsvs nddeedplip frrsnetpet rqrrlsgkkv
     1141 rgilmdllrg cgdcrsssst nlikneqqtk eapytvngpp qikitspsmg ptssgstqcn
     1201 ercchayalr iesqleefrt emervrsrsd aildmlnmlh svdmn
//
LOCUS       XP_034172376            1178 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600701 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172376
VERSION     XP_034172376.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172376.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1178
                     /product="uncharacterized protein LOC117600701 isoform X2"
                     /calculated_mol_wt=134613
     CDS             1..1178
                     /gene="LOC117600701"
                     /coded_by="XM_034316485.2:158..3694"
                     /db_xref="GeneID:117600701"
ORIGIN      
        1 mseenkhtse etesemnten qireqsksdt silnneseqp mledeemptf vsvsspsrrd
       61 dsdpsnvkqq cripveedhs rspcpsddqg ggsiyvlssg eesnhpynde eedeygdsde
      121 mdemdleneh ehehgmlede eeedeeddde rnplrmpned dedidteydq eyfdrmvpvk
      181 yqhveskvkq yirdikeqnk rsmekrikeq efvenktyee denndteqvn tpvinkvikd
      241 yaqktikdlq reeiegtnlv yniaqivkns knnktdnkvi enqfkhmrkd seyvniqnek
      301 raqssddkle krngsvdinv hqieyqntld rnikfnnmnq splvngheqt pafinlrtvt
      361 yeeymhgitn seqkdeldeq kekahetevy neaemineqe idnsntscsl kiesvksirm
      421 sqdesgnlnf akvnltddiv testeiiqlk tqlsqkdaqf hhlkdayqkt laenikmkqe
      481 ldalkqslak yenenkaret kvasvqtetv eksvcnqett ttgeinkkvs tssvastass
      541 sdqwaesays pavsikppdl tsilnsddsi vltnatprki ahplsrtfit ssrilqtlsn
      601 itqgktktes plvqntkkgl nenltdqlvn shysspthts sskkrkatem lgsstftqpf
      661 kiphtslesd rksnintanv efkfveehak skaeqeqnas inknidgsts tdskteetvd
      721 ndngvkcfiy aedehsknks fliqaeeplk dgineknrvq ecgpyllgnl evrmseingt
      781 isvwgkevnr ddindneddt evsitpsekk tcncwqstpe mrfnesplvc stnkklrips
      841 rfnrsnsnss qyrrnlssnv mgtsslgddl hdkihtdhsf nlnncvlsce kydspkynee
      901 wpickgasss qeklhsccih ppelvneerr cdlhhekqky dlspkhlknk lykgirrnsy
      961 kntfipdskn rlndnvtntn ietnevtskc rtcqrsfqnv vanpthskfs nivrnfsysc
     1021 kssldnisnh ensekhtcsh svsnddeedp lipfrrsnet petrqrrlsg kkvrgilmdl
     1081 lrgcgdcrss sstnlikneq qtkeapytvn gppqikitsp smgptssgst qcnercchay
     1141 alriesqlee frtemervrs rsdaildmln mlhsvdmn
//
LOCUS       XP_034172386            1003 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600701 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172386
VERSION     XP_034172386.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316495.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172386.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1003
                     /product="uncharacterized protein LOC117600701 isoform X3"
                     /calculated_mol_wt=114236
     CDS             1..1003
                     /gene="LOC117600701"
                     /coded_by="XM_034316495.2:141..3152"
                     /db_xref="GeneID:117600701"
ORIGIN      
        1 mvpvkyqhve skvkqyirdi keqnkrsmek rikeqefven ktyeedennd teqvntpvin
       61 kvikdyaqkt ikdlqreeie gtnlvyniaq ivknsknnkt dnkvienqfk hmrkdseyvn
      121 iqnekraqss ddklekrngs vdinvhqiey qntldrnikf nnmnqsplvn gheqtpafin
      181 lrtvtyeeym hgitnseqkd eldeqkekah etevyneaem ineqeidnsn tscslkiesv
      241 ksirmsqdes gnlnfakvnl tddivteste iiqlktqlsq kdaqfhhlkd ayqktlaeni
      301 kmkqeldalk qslakyenen karetkvasv qtetveksvc nqettttgei nkkvstssva
      361 stasssdqwa esayspavsi kppdltsiln sddsivltna tprkiahpls rtfitssril
      421 qtlsnitqgk tktesplvqn tkkglnenlt dqlvnshyss pthtssskkr katemlgsst
      481 ftqpfkipht slesdrksni ntanvefkfv eehakskaeq eqnasinkni dgststdskt
      541 eetvdndngv kcfiyaedeh sknksfliqa eeplkdgine knrvqecgpy llgnlevrms
      601 eingtisvwg kevnrddind neddtevsit psekktcncw qstpemrfne splvcstnkk
      661 lripsrfnrs nsnssqyrrn lssnvmgtss lgddlhdkih tdhsfnlnnc vlscekydsp
      721 kyneewpick gasssqeklh sccihppelv neerrcdlhh ekqkydlspk hlknklykgi
      781 rrnsykntfi pdsknrlndn vtntnietne vtskcrtcqr sfqnvvanpt hskfsnivrn
      841 fsysckssld nisnhensek htcshsvsnd deedplipfr rsnetpetrq rrlsgkkvrg
      901 ilmdllrgcg dcrsssstnl ikneqqtkea pytvngppqi kitspsmgpt ssgstqcner
      961 cchayalrie sqleefrtem ervrsrsdai ldmlnmlhsv dmn
//
LOCUS       XP_034172400            1180 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600724 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172400
VERSION     XP_034172400.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316509.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1180
                     /product="uncharacterized protein LOC117600724 isoform X1"
                     /calculated_mol_wt=133260
     Region          <776..1129
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1180
                     /gene="LOC117600724"
                     /coded_by="XM_034316509.2:294..3836"
                     /db_xref="GeneID:117600724"
ORIGIN      
        1 mkqrccvegk imkkrrpctw nssrlakssp rpfhvrefdn fptetksfhe lkhlqkynsk
       61 sqkknaklne sslkrdafgs leslfdlkkp lnlpeihsft ieyhqeghvp lyksksfkns
      121 khivesvllq qtaelephil ertheaddhk rkeeedkqep nletletqer ksensetkes
      181 lnmaenesqe fpdnksdris lskksssrvg srhsglgskh tevgsrhtev gdkgleatik
      241 eisntgtvgq wdqvppfypl ppgvgegasi kpggrqesml ssspftdstv klemknader
      301 cavlakdieq lkleiaelsk kkdltsedtl tiqkkqdevm rklaefeqit rklqrllgla
      361 dvsstafakm fdmipqpysk lslaiaeeee evaaqekird kidkevsatr iskpedrfdl
      421 pqieypedkl prvivcgnte deipkivvad skkkgkeslq tltgkltesl tmqeklaken
      481 aqleggkykl eeallekdsa veslqrkvcg lqaemrivvk enveltrqia clnqrvtspc
      541 cytcpgaiss gvsspcplrs vsytttlqqd ddycrckccl qpdslspian atsvkagsht
      601 cvpsedsprl aggasvtgac itsgassqid emccrspava kspcvqpppk svcpaeienk
      661 iaayessnkq leqqlgsmec evrnmqmela nvqrerqqle qqrkllkcta pcapcgccpp
      721 spmtlqgssp ppyvppptlp pvlppsssvp lskmppppts tctgscinap mgastcpqqq
      781 lrdlreqyar lqddyknklc evsclrtdae klkqqtreti eekekldikl idaqerlkal
      841 eaekdkyegf keqmieqeqa livfkqrfre aqdeleelrs miqdqaaqle dyrnkylqaq
      901 qqveeqrrql dlmemdnarm nenvtleigr vknqfqekla elaplpdilk qtqvklqeaq
      961 qmravaernc edlsrellat kdkmqalqnq ldvmhnefqq lqderghgsg rfdelerkna
     1021 elrhenermk ntlarfeehe aqlqkridek mhevtqltam ldqvredsar qvartkerce
     1081 tarrtmqsqi aemerqlaqc rataraaqkd rdeirqkmqg qinnlneafe qaqgrirslq
     1141 ghvnylktsy snifkgqgem ppgvlpgeag tgfdscdcny
//
LOCUS       XP_034172419             998 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600724 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172419
VERSION     XP_034172419.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316528.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..998
                     /product="uncharacterized protein LOC117600724 isoform X2"
                     /calculated_mol_wt=111806
     Region          <594..947
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..998
                     /gene="LOC117600724"
                     /coded_by="XM_034316528.2:261..3257"
                     /db_xref="GeneID:117600724"
ORIGIN      
        1 maenesqefp dnksdrisls kksssrvgsr hsglgskhte vgsrhtevgd kgleatikei
       61 sntgtvgqwd qvppfyplpp gvgegasikp ggrqesmlss spftdstvkl emknaderca
      121 vlakdieqlk leiaelskkk dltsedtlti qkkqdevmrk laefeqitrk lqrllgladv
      181 sstafakmfd mipqpyskls laiaeeeeev aaqekirdki dkevsatris kpedrfdlpq
      241 ieypedklpr vivcgntede ipkivvadsk kkgkeslqtl tgkltesltm qeklakenaq
      301 leggkyklee allekdsave slqrkvcglq aemrivvken veltrqiacl nqrvtspccy
      361 tcpgaissgv sspcplrsvs ytttlqqddd ycrckcclqp dslspianat svkagshtcv
      421 psedsprlag gasvtgacit sgassqidem ccrspavaks pcvqpppksv cpaeienkia
      481 ayessnkqle qqlgsmecev rnmqmelanv qrerqqleqq rkllkctapc apcgccppsp
      541 mtlqgssppp yvppptlppv lppsssvpls kmpppptstc tgscinapmg astcpqqqlr
      601 dlreqyarlq ddyknklcev sclrtdaekl kqqtretiee kekldiklid aqerlkalea
      661 ekdkyegfke qmieqeqali vfkqrfreaq deleelrsmi qdqaaqledy rnkylqaqqq
      721 veeqrrqldl memdnarmne nvtleigrvk nqfqeklael aplpdilkqt qvklqeaqqm
      781 ravaernced lsrellatkd kmqalqnqld vmhnefqqlq derghgsgrf delerknael
      841 rhenermknt larfeeheaq lqkridekmh evtqltamld qvredsarqv artkerceta
      901 rrtmqsqiae merqlaqcra taraaqkdrd eirqkmqgqi nnlneafeqa qgrirslqgh
      961 vnylktsysn ifkgqgempp gvlpgeagtg fdscdcny
//
LOCUS       XP_034172409             998 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600724 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172409
VERSION     XP_034172409.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316518.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..998
                     /product="uncharacterized protein LOC117600724 isoform X2"
                     /calculated_mol_wt=111806
     Region          <594..947
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..998
                     /gene="LOC117600724"
                     /coded_by="XM_034316518.2:261..3257"
                     /db_xref="GeneID:117600724"
ORIGIN      
        1 maenesqefp dnksdrisls kksssrvgsr hsglgskhte vgsrhtevgd kgleatikei
       61 sntgtvgqwd qvppfyplpp gvgegasikp ggrqesmlss spftdstvkl emknaderca
      121 vlakdieqlk leiaelskkk dltsedtlti qkkqdevmrk laefeqitrk lqrllgladv
      181 sstafakmfd mipqpyskls laiaeeeeev aaqekirdki dkevsatris kpedrfdlpq
      241 ieypedklpr vivcgntede ipkivvadsk kkgkeslqtl tgkltesltm qeklakenaq
      301 leggkyklee allekdsave slqrkvcglq aemrivvken veltrqiacl nqrvtspccy
      361 tcpgaissgv sspcplrsvs ytttlqqddd ycrckcclqp dslspianat svkagshtcv
      421 psedsprlag gasvtgacit sgassqidem ccrspavaks pcvqpppksv cpaeienkia
      481 ayessnkqle qqlgsmecev rnmqmelanv qrerqqleqq rkllkctapc apcgccppsp
      541 mtlqgssppp yvppptlppv lppsssvpls kmpppptstc tgscinapmg astcpqqqlr
      601 dlreqyarlq ddyknklcev sclrtdaekl kqqtretiee kekldiklid aqerlkalea
      661 ekdkyegfke qmieqeqali vfkqrfreaq deleelrsmi qdqaaqledy rnkylqaqqq
      721 veeqrrqldl memdnarmne nvtleigrvk nqfqeklael aplpdilkqt qvklqeaqqm
      781 ravaernced lsrellatkd kmqalqnqld vmhnefqqlq derghgsgrf delerknael
      841 rhenermknt larfeeheaq lqkridekmh evtqltamld qvredsarqv artkerceta
      901 rrtmqsqiae merqlaqcra taraaqkdrd eirqkmqgqi nnlneafeqa qgrirslqgh
      961 vnylktsysn ifkgqgempp gvlpgeagtg fdscdcny
//
LOCUS       XP_034172432             843 aa            linear   INV 03-SEP-2025
DEFINITION  ftz transcription factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034172432
VERSION     XP_034172432.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316541.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172432.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..843
                     /product="ftz transcription factor 1"
                     /calculated_mol_wt=87029
     Region          110..>150
                     /region_name="NR_DBD_like"
                     /note="DNA-binding domain of nuclear receptors is composed
                     of two C4-type zinc fingers; cl02596"
                     /db_xref="CDD:413390"
     Region          377..469
                     /region_name="NR_DBD_Lrh-1_like"
                     /note="The DNA-binding domain of Lrh-1 like nuclear
                     receptor family like is composed of two C4-type zinc
                     fingers; cd07167"
                     /db_xref="CDD:143541"
     Site            order(377,380,394,397,413,419,429,432)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143541"
     Site            order(387..389,395..396,398,400,402..403,406,426..427,433,
                     446..448,451..457,463)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143541"
     Region          611..841
                     /region_name="NR_LBD_Ftz-F1_like"
                     /note="The ligand binding domain of FTZ-F1 like nuclear
                     receptors; cd06944"
                     /db_xref="CDD:132742"
     Site            order(647,650..651,687..688,691,695,710,729..730,815,
                     818..819,822)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132742"
     Site            order(659,662,666,676,679..680,683..684,832..833,836..837)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132742"
     CDS             1..843
                     /gene="ftz-f1"
                     /coded_by="XM_034316541.2:1..2532"
                     /db_xref="GeneID:117600735"
ORIGIN      
        1 mllemeqhsg lislnmspfh lspqgspqga agagqqqqqq qqqqaqqqqp qygsspygnc
       61 aqsppttmch qsqsqqqqqh lsvhsqahsq ggissfdaaf fdstaleerc pmcgdkmsgy
      121 qyglltcesc kgffkrnnsp csskkvytcl fsptgggggg gggggggggg gggggggggg
      181 ssggggnsgs gnnggnggcg gassaleigs cgnkkvytcl fsptgagsgt ggsggnngng
      241 agggcgtsta lesssssytg gasgtggggg gggggggggg aggggagaag gsgptagnaa
      301 aggggggnas gaggagsggg satvsgnvai ptttyslpsg tlchpglgqv gvgvvtgsip
      361 cpsdfpdtkd ivieelcpvc gdkvsgyhyg lltcesckgf fkrtvqnkkv ytcvaersch
      421 idktqrkrcp ycrfqkclev gmkleavrad rmrggrnkfg pmykrdrark lqmmrqrqla
      481 lqtirgslgd psnypsavtp flhikqeiqi pqvssltssp dsspspaava aglvttqags
      541 gagqhqliap ssqpnisggn hlhnlnpgld sklwtanstt pspkafnfge qstqphgptg
      601 sapstatlkt spmirdfvqt vddrewqtsl ygllqsqtyn qcevdlfelm ckvldqnlfs
      661 qvdwarnsvf fkdlkvddqm kllqhswsdm lvldhlhqrl hnslpdettl hngqkfdllc
      721 lgllgvpsla dlfndlsskl qelkfdpsdy icmkflmlln hevrglvnkk hvqegheqvq
      781 qalldytltc ypsipdkfnk llavlpeihv vasrgedhly qkhcsggapt qtllmemlha
      841 krk
//
LOCUS       XP_034172456             540 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger and WD repeat domain 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172456
VERSION     XP_034172456.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316565.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172456.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="ring finger and WD repeat domain 3 isoform X1"
                     /calculated_mol_wt=60735
     Region          69..132
                     /region_name="mRING-C3HGC3_RFWD3"
                     /note="Modified RING finger, C3HGC3-type, found in RING
                     finger and WD repeat domain-containing protein 3 (RFWD3)
                     and similar proteins; cd16450"
                     /db_xref="CDD:438114"
     Region          123..>197
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          <229..>367
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     CDS             1..540
                     /gene="Rfwd3"
                     /coded_by="XM_034316565.2:329..1951"
                     /db_xref="GeneID:117600739"
ORIGIN      
        1 mevmdyreep pemedagedh seesdnndnv dnnpdpeiqi ieekkdehde skvkrpkevs
       61 ldesetdsdq scpicmdlwt ssgdhrlccl rcghlfgync ilrwlqnsct tgnrrcpqcn
      121 rkaavkdirm lyakklasid taeldklkeq fnnvkseknr iemelsrytl rqklleqqis
      181 smrnritele nqqseisvhs cqniskhmik kfhldrsiei ckdggcrvld ynpslgilva
      241 sqkssstlfs gygikkidte kfqprqfifl hsqairdvtf ntnqqsllls vgfdkcaklm
      301 diqnhvtmht fqedsplwsc cwsgdnpnif fvgaqngsit qydirqtssa vesldspgdr
      361 spvvslatvp anpgsgisrg gfiacrlntc yayeqkesky ipkqiflegp fvsvcydekn
      421 nhtlissrpn arqpharhvv ctiekgsdes ticnvvhtfh agssqqllsr pcyinvendt
      481 lvaahqesss sislwstssg kqvnslpvsd pvvdmcavei sntlflatls tkkvriyshg
//
LOCUS       XP_034172465             528 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger and WD repeat domain 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172465
VERSION     XP_034172465.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172465.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..528
                     /product="ring finger and WD repeat domain 3 isoform X2"
                     /calculated_mol_wt=59228
     Region          57..120
                     /region_name="mRING-C3HGC3_RFWD3"
                     /note="Modified RING finger, C3HGC3-type, found in RING
                     finger and WD repeat domain-containing protein 3 (RFWD3)
                     and similar proteins; cd16450"
                     /db_xref="CDD:438114"
     Region          111..>185
                     /region_name="DR0291"
                     /note="Predicted nucleic acid-binding protein DR0291,
                     contains C4-type Zn-ribbon domain [General function
                     prediction only]; COG1579"
                     /db_xref="CDD:441187"
     Region          <217..>355
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     CDS             1..528
                     /gene="Rfwd3"
                     /coded_by="XM_034316574.2:167..1753"
                     /db_xref="GeneID:117600739"
ORIGIN      
        1 medagedhse esdnndnvdn npdpeiqiie ekkdehdesk vkrpkevsld esetdsdqsc
       61 picmdlwtss gdhrlcclrc ghlfgyncil rwlqnscttg nrrcpqcnrk aavkdirmly
      121 akklasidta eldklkeqfn nvkseknrie melsrytlrq klleqqissm rnritelenq
      181 qseisvhscq niskhmikkf hldrsieick dggcrvldyn pslgilvasq kssstlfsgy
      241 gikkidtekf qprqfiflhs qairdvtfnt nqqslllsvg fdkcaklmdi qnhvtmhtfq
      301 edsplwsccw sgdnpniffv gaqngsitqy dirqtssave sldspgdrsp vvslatvpan
      361 pgsgisrggf iacrlntcya yeqkeskyip kqiflegpfv svcydeknnh tlissrpnar
      421 qpharhvvct iekgsdesti cnvvhtfhag ssqqllsrpc yinvendtlv aahqessssi
      481 slwstssgkq vnslpvsdpv vdmcaveisn tlflatlstk kvriyshg
//
LOCUS       XP_034172477             416 aa            linear   INV 03-SEP-2025
DEFINITION  medium-chain acyl-CoA dehydrogenase [Osmia lignaria lignaria].
ACCESSION   XP_034172477
VERSION     XP_034172477.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316586.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="medium-chain acyl-CoA dehydrogenase"
                     /calculated_mol_wt=45588
     Region          32..408
                     /region_name="ACAD"
                     /note="Acyl-CoA dehydrogenase; cl09933"
                     /db_xref="CDD:447864"
     Site            order(119,149,151,182,184,391..393,395,397)
                     /site_type="active"
                     /db_xref="CDD:173838"
     CDS             1..416
                     /gene="Mcad"
                     /coded_by="XM_034316586.2:240..1490"
                     /db_xref="GeneID:117600753"
ORIGIN      
        1 mfsrsilkki thqkvmrafs tsdlatgynf elnetqkemq elarkftkee iipvaaehdr
       61 tgkypwdiik kawglgllnk hipqhcggme vstfdgclva eefaygctgi stalegsglg
      121 qapviaagtk eqhkkylgrl leeplvaayc vtepaagsdv agiktkaekk gkewilngtk
      181 mwitnggvan wyfvlarsnp dpkapagkaf tafiverese gltpgrkein mgqrasdtrm
      241 itfedvrvpe envlgkegeg fkiamktfdk trpmvasasv glaqraldea tkyslerktf
      301 dkviaehqav afmlsdmsia vetarlawmk sawaadnnlp satllasiak cyggdvankc
      361 atdavqifgg agfnseypve klmrdakiyq iyegtaqiqr liisrhllnq akqrat
//
LOCUS       XP_034172492             393 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoribosyl pyrophosphate synthetase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172492
VERSION     XP_034172492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="phosphoribosyl pyrophosphate synthetase isoform
                     X1"
                     /calculated_mol_wt=43156
     Region          52..389
                     /region_name="PrsA"
                     /note="Phosphoribosylpyrophosphate synthetase [Nucleotide
                     transport and metabolism]; COG0462"
                     /db_xref="CDD:440230"
     CDS             1..393
                     /gene="Prps"
                     /coded_by="XM_034316601.2:107..1288"
                     /db_xref="GeneID:117600759"
ORIGIN      
        1 mdlleqrkik rvchcsqkwk tnvmpvsqpv raksllranl essrgqflqs rmpnikvfsg
       61 tshpdlaqri vdrlgidigk vvtkkfsnle tcveigesvr gedvyivqsg sgevndnlme
      121 lliminacki asasrvtavi pcfpyarqdk kdkggdggdn snskkqivmk snewkfrsra
      181 pisaklvanm lsvagadhii tmdlhasqiq gffdipvdnl faepavlkwi kenivewrns
      241 iivspdagga krvtsiadrl nvefalihke rkkanevasm vlvgdvkdrv ailvddmadt
      301 cgtichaaek lleagatkvy ailthgifsg paisrinnac feavvvtnti pqdghmkdcp
      361 kiqcidvsmm faeavrrthn gesvsylfsn vpy
//
LOCUS       XP_034172502             377 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoribosyl pyrophosphate synthetase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172502
VERSION     XP_034172502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..377
                     /product="phosphoribosyl pyrophosphate synthetase isoform
                     X2"
                     /calculated_mol_wt=41394
     Region          52..373
                     /region_name="PrsA"
                     /note="Phosphoribosylpyrophosphate synthetase [Nucleotide
                     transport and metabolism]; COG0462"
                     /db_xref="CDD:440230"
     CDS             1..377
                     /gene="Prps"
                     /coded_by="XM_034316611.2:123..1256"
                     /db_xref="GeneID:117600759"
ORIGIN      
        1 mdlleqrkik rvchcsqkwk tnvmpvsqpv raksllranl essrgqflqs rmpnikvfsg
       61 tshpdlaqri vdrlgidigk vvtkkfsnle tcveigesvr gedvyivqsg sgevndnlme
      121 lliminacki asasrvtavi pcfpyarqdk kdkdflpels tsrapisakl vanmlsvaga
      181 dhiitmdlha sqiqgffdip vdnlfaepav lkwikenive wrnsiivspd aggakrvtsi
      241 adrlnvefal ihkerkkane vasmvlvgdv kdrvailvdd madtcgtich aaeklleaga
      301 tkvyailthg ifsgpaisri nnacfeavvv tntipqdghm kdcpkiqcid vsmmfaeavr
      361 rthngesvsy lfsnvpy
//
LOCUS       XP_034172517             369 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoribosyl pyrophosphate synthetase isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172517
VERSION     XP_034172517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316626.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="phosphoribosyl pyrophosphate synthetase isoform
                     X3"
                     /calculated_mol_wt=40491
     Region          52..365
                     /region_name="PrsA"
                     /note="Phosphoribosylpyrophosphate synthetase [Nucleotide
                     transport and metabolism]; COG0462"
                     /db_xref="CDD:440230"
     CDS             1..369
                     /gene="Prps"
                     /coded_by="XM_034316626.2:108..1217"
                     /db_xref="GeneID:117600759"
ORIGIN      
        1 mdlleqrkik rvchcsqkwk tnvmpvsqpv raksllranl essrgqflqs rmpnikvfsg
       61 tshpdlaqri vdrlgidigk vvtkkfsnle tcveigesvr gedvyivqsg sgevndnlme
      121 lliminacki asasrvtavi pcfpyarqdk kdksrapisa klvanmlsva gadhiitmdl
      181 hasqiqgffd ipvdnlfaep avlkwikeni vewrnsiivs pdaggakrvt siadrlnvef
      241 alihkerkka nevasmvlvg dvkdrvailv ddmadtcgti chaaekllea gatkvyailt
      301 hgifsgpais rinnacfeav vvtntipqdg hmkdcpkiqc idvsmmfaea vrrthngesv
      361 sylfsnvpy
//
LOCUS       XP_034172531             299 aa            linear   INV 03-SEP-2025
DEFINITION  metallophosphoesterase domain-containing protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172531
VERSION     XP_034172531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316640.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..299
                     /product="metallophosphoesterase domain-containing protein
                     1"
                     /calculated_mol_wt=33084
     Region          43..273
                     /region_name="MPP_239FB"
                     /note="Homo sapiens 239FB and related proteins,
                     metallophosphatase domain; cd07379"
                     /db_xref="CDD:277325"
     Site            order(49,51,72,103..104,216,254,256)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:277325"
     Site            order(49,51,72,103,216,254,256)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:277325"
     CDS             1..299
                     /gene="LOC117600769"
                     /coded_by="XM_034316640.2:421..1320"
                     /db_xref="GeneID:117600769"
ORIGIN      
        1 msieihplta nptaawrels qqqkvikina klptteapnd klrivcmsdt hsltpfikfd
       61 ipmgdvfiha gdftkcgslq eviefnnwig tlphknkivi agnhelsfdp tftnpfslht
      121 sgdrqkhtgt sildniptlg mskdtlaeai qttnvkdylt nctyledsei iingikiygt
      181 pwqpefckwa fnvprgeacl skwemipsdt dilithtppv ghgdlccsgv ragcvellst
      241 vqnrvkpkyh vfghihegyg issdgkiiyi nastcdlnyl psnppvvfdi tlppdhckl
//
LOCUS       XP_034172545             199 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin conjugating enzyme 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172545
VERSION     XP_034172545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316654.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="ubiquitin conjugating enzyme 4 isoform X1"
                     /calculated_mol_wt=22314
     Region          6..149
                     /region_name="UBCc_UBE2K"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 K and related
                     proteins; cd23800"
                     /db_xref="CDD:467420"
     Region          163..198
                     /region_name="UBA_II_E2_UBCD4"
                     /note="UBA domain found in Drosophila melanogaster
                     ubiquitin-conjugating enzyme E2-22 kDa (UbcD4) and similar
                     proteins; cd14391"
                     /db_xref="CDD:270574"
     Site            order(171..174,191,194,198)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270574"
     CDS             1..199
                     /gene="Ubc4"
                     /coded_by="XM_034316654.2:77..676"
                     /db_xref="GeneID:117600774"
ORIGIN      
        1 maniaaqrik refkevikse evakcaikve lvndsftelk geiagppdtp yeggnfvlei
       61 kvpetypfnp pkvrfitkiw hpnissvtga icldilkdqw aaamtlrtvl lslqallsaa
      121 epddpqdavv akqykehpem frqtakhwtf vyaggpakmp dlddkirrlt dmgieehnar
      181 valssynwdl erateqlfs
//
LOCUS       XP_034172554             189 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin conjugating enzyme 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172554
VERSION     XP_034172554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316663.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..189
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..189
                     /product="ubiquitin conjugating enzyme 4 isoform X2"
                     /calculated_mol_wt=21159
     Region          13..139
                     /region_name="UBCc_UBE2K"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 K and related
                     proteins; cd23800"
                     /db_xref="CDD:467420"
     Region          153..188
                     /region_name="UBA_II_E2_UBCD4"
                     /note="UBA domain found in Drosophila melanogaster
                     ubiquitin-conjugating enzyme E2-22 kDa (UbcD4) and similar
                     proteins; cd14391"
                     /db_xref="CDD:270574"
     Site            order(161..164,181,184,188)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270574"
     CDS             1..189
                     /gene="Ubc4"
                     /coded_by="XM_034316663.2:106..675"
                     /db_xref="GeneID:117600774"
ORIGIN      
        1 mlrllnighn rvakcaikve lvndsftelk geiagppdtp yeggnfvlei kvpetypfnp
       61 pkvrfitkiw hpnissvtga icldilkdqw aaamtlrtvl lslqallsaa epddpqdavv
      121 akqykehpem frqtakhwtf vyaggpakmp dlddkirrlt dmgieehnar valssynwdl
      181 erateqlfs
//
LOCUS       XP_034172563             179 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin conjugating enzyme 4 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172563
VERSION     XP_034172563.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316672.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..179
                     /product="ubiquitin conjugating enzyme 4 isoform X3"
                     /calculated_mol_wt=19972
     Region          3..129
                     /region_name="UBCc_UBE2K"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-conjugating enzyme E2 K and related
                     proteins; cd23800"
                     /db_xref="CDD:467420"
     Region          143..178
                     /region_name="UBA_II_E2_UBCD4"
                     /note="UBA domain found in Drosophila melanogaster
                     ubiquitin-conjugating enzyme E2-22 kDa (UbcD4) and similar
                     proteins; cd14391"
                     /db_xref="CDD:270574"
     Site            order(151..154,171,174,178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270574"
     CDS             1..179
                     /gene="Ubc4"
                     /coded_by="XM_034316672.2:141..680"
                     /db_xref="GeneID:117600774"
ORIGIN      
        1 mvakcaikve lvndsftelk geiagppdtp yeggnfvlei kvpetypfnp pkvrfitkiw
       61 hpnissvtga icldilkdqw aaamtlrtvl lslqallsaa epddpqdavv akqykehpem
      121 frqtakhwtf vyaggpakmp dlddkirrlt dmgieehnar valssynwdl erateqlfs
//
LOCUS       XP_034172575             164 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600789 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172575
VERSION     XP_034172575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..164
                     /product="uncharacterized protein LOC117600789 isoform X1"
                     /calculated_mol_wt=17942
     CDS             1..164
                     /gene="LOC117600789"
                     /coded_by="XM_034316684.2:1785..2279"
                     /db_xref="GeneID:117600789"
ORIGIN      
        1 mhegrmatgt geggghtale nttataltes tssssstvpt iltenntaan lpltgpqqqp
       61 nlhqvqqqsh srprvnlvtd lptndasvsk mglqsgavlg tlhtprhvar rarerereqk
      121 ekeryitltr krekdhtsli ffasnitsyr yhtciantei viar
//
LOCUS       XP_034172583             103 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600789 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172583
VERSION     XP_034172583.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316692.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..103
                     /product="uncharacterized protein LOC117600789 isoform X2"
                     /calculated_mol_wt=10826
     CDS             1..103
                     /gene="LOC117600789"
                     /coded_by="XM_034316692.2:1785..2096"
                     /db_xref="GeneID:117600789"
ORIGIN      
        1 mhegrmatgt geggghtale nttataltes tssssstvpt iltenntaan lpltgpqqqp
       61 nlhqvqqqsh srprvnlvtd lptndasdgn ktcapafqry rrk
//
LOCUS       XP_076546102              97 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600789 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546102
VERSION     XP_076546102.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689987.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..97
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..97
                     /product="uncharacterized protein LOC117600789 isoform X3"
                     /calculated_mol_wt=10027
     CDS             1..97
                     /gene="LOC117600789"
                     /coded_by="XM_076689987.1:1785..2078"
                     /db_xref="GeneID:117600789"
ORIGIN      
        1 mhegrmatgt geggghtale nttataltes tssssstvpt iltenntaan lpltgpqqqp
       61 nlhqvqqqsh srprvnlvtd lptndasvca rafisfv
//
LOCUS       XP_034172593             102 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-related modifier 1 [Osmia lignaria lignaria].
ACCESSION   XP_034172593
VERSION     XP_034172593.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316702.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="ubiquitin-related modifier 1"
                     /calculated_mol_wt=11330
     Region          10..102
                     /region_name="Ubl_Urm1"
                     /note="ubiquitin-like (Ubl) domain found in
                     ubiquitin-related modifier 1 (Urm1); cd01764"
                     /db_xref="CDD:340463"
     CDS             1..102
                     /gene="Urm1"
                     /coded_by="XM_034316702.2:166..474"
                     /db_xref="GeneID:117600800"
ORIGIN      
        1 msnenseisv tiefgggael lfdkkkkhkv nlpgdewtiq kllfwikdnl lkerpelflq
       61 gntvrpgilv lvndtdwell gegsyklcsg dtilfistlh gg
//
LOCUS       XP_034186202             327 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607073 [Osmia lignaria lignaria].
ACCESSION   XP_034186202
VERSION     XP_034186202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="uncharacterized protein LOC117607073"
                     /calculated_mol_wt=35955
     CDS             1..327
                     /gene="LOC117607073"
                     /coded_by="XM_034330311.2:190..1173"
                     /db_xref="GeneID:117607073"
ORIGIN      
        1 maqwmsfvll vvattiavte sraipaepis vmldpygnpi vflrekrtav hpyphrammf
       61 tgyyrpvrrs nhagqatgvf aqgnavsgea ffggmqaphl kngpepieep evssaeaqaa
      121 pepehtydeq eqtqvqeheg yqpeehhhyh qeeghypqee eqhhqeeeeh qkqhlppaqe
      181 qpepepapvy tteaapvtpa eepvhtvpev vtnrpkvqqg kktkktpvvv eeedddeeyd
      241 depsvpfipf kgnrrrqnyp nlnnffpmvf sfpggssrsg apgsppgavt aiansystak
      301 ggvassiata yggspngkkr paptsee
//
LOCUS       XP_076546103            1053 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305646 [Osmia lignaria lignaria].
ACCESSION   XP_076546103
VERSION     XP_076546103.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689988.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1053
                     /product="uncharacterized protein LOC143305646"
                     /calculated_mol_wt=121746
     CDS             1..1053
                     /gene="LOC143305646"
                     /coded_by="XM_076689988.1:173..3334"
                     /db_xref="GeneID:143305646"
ORIGIN      
        1 mnkrkasadr nskqrgssrr psasslhsrp ntahqklkpe kspstqnlvt pepqnlskti
       61 qvskavetcv pktrfssqdr crelrsyssp rlkyfpaeri krfesppgtp igaliaqqnl
      121 hesgsyyaag hpeynngqgd attasqslhq hrppqhvqsv gqqqvagnqv aqmnphsdpt
      181 lpqqilsqsh rpfvphgprd hspqktianv hnshsayhnp scamttpqpv asmmeqernt
      241 seeptithpp dyerqpiggr naanyrqain kqqiqlqsqm smdpnasnlt psdhqqlqnh
      301 ysrhgqfsqn pqqvyqqmqq nmqqqmqmqq ramtyqmqam ggqgqgpyav qygryfpgqg
      361 mttpqdatvn qmknlrtgqs ygnqghqrdl dysmnhqngw akgnqvgefs sikaqlnpyt
      421 vevtnnnqph hgthpyhhav hqhrplyknp gqgtpmmyhq pgfdqqipan qqmpqqppte
      481 nnqvpkndnk ksppfthsml rdqekllntm kqqgvpldim rrqfdillne qkkhleylec
      541 lwrqdeeevr rlpvinrkkr nidekpewmt hltptrmsyl emerlreqqk keqearlqqq
      601 nanemvnqqp plqlqnqqis qlqsqqihqi qnqqappqlq nqqipqlqnq qappqlqnqq
      661 pyqlwqpnwq qsrisqpynh mypqpymnqg pmnyrhqqsm pdyryahqqy nhqhqqqhyq
      721 yvpqqnqeei qqtngysgyq dvrqptepss lmkirhyknh vrtqkrnngl qdpevarrql
      781 eelranselr kgleylanfa pkkatfrlng iqdrnetdte iqnrlpvlna epavkrvsan
      841 glensriann pppqrlspln klehefakey prqreptprn cysvpaeren gtmaseqqpq
      901 ylpqqlpven sanlisydkn ptmargnvim pykfdvnypq hyqqmqqyyq ntknltgnng
      961 egdvgvsqki emnrknfera ggdayenmng qvmqegkipm qgmqqygqpd mheartiggv
     1021 rylarkqdyl pnqqivspet liasrhlqpp iiy
//
LOCUS       XP_034172732             209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600853 [Osmia lignaria lignaria].
ACCESSION   XP_034172732
VERSION     XP_034172732.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316841.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172732.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="uncharacterized protein LOC117600853"
                     /calculated_mol_wt=23120
     Region          102..153
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..209
                     /gene="LOC117600853"
                     /coded_by="XM_034316841.2:56..685"
                     /db_xref="GeneID:117600853"
ORIGIN      
        1 mgkmrikvsi altivlavgh rsragviple esfheqheps deatlgqqgp lassyvqfhg
       61 pvegpefavk vpymvhhgds nhlhgqeqqn gytfdyvahp kyefsygved eqsgdfhsqk
      121 esrdgssvsg qysvkepggs irvvsyradk ngfhavvhts gkndhsvgih hgqgrtrghe
      181 hqvamqqeqq eqqeqqahdy asytendgy
//
LOCUS       XP_034196317             494 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box protein crocodile [Osmia lignaria lignaria].
ACCESSION   XP_034196317
VERSION     XP_034196317.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340426.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="forkhead box protein crocodile"
                     /calculated_mol_wt=54413
     Region          69..159
                     /region_name="FH_FOXL1"
                     /note="Forkhead (FH) domain found in Forkhead box protein
                     L1 (FOXL1) and similar proteins; cd20027"
                     /db_xref="CDD:410801"
     Site            order(75,93..94,97,116..117,119..120,123,130,140..142,144)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410801"
     CDS             1..494
                     /gene="croc"
                     /coded_by="XM_034340426.2:692..2176"
                     /db_xref="GeneID:117611924"
ORIGIN      
        1 mhtlfgeqna yyrhatavpv gmgtpsypgv gaapgyyeqy ryggyataag ypvsgitqqh
       61 ihhpgkdmvk ppysyialia maiqnapdkk itlngiyqfi merfpyyren kqgwqnsirh
      121 nlslnecfvk vprddkkpgk gsywsldpds ynmfdngsyl rrrrrfkkkd alkekeealk
      181 rqglvpekrn qeeakpsniv ipppsdtsnk klntlettlc kpkrepvnda gshcmavqak
      241 yglhspiqdt kttaatttaa vavpgqsviq talghqvhpa hqvhqdaiqd vsmgldptsf
      301 svdalmttre nsatlmtren qhhthhphlq hphphshsim tsresmgagv vsrdhlsavc
      361 tttttttaav aammattgyg hgstvsrsng sppgtmyapy ctptagyimd haeynsrnhn
      421 ntaghsqwyq eaaspdtaai yqdtqspscq lyrsspptpl sssaapsppl yhryyqdcnt
      481 vntsgnlpit lhky
//
LOCUS       XP_034171908             760 aa            linear   INV 03-SEP-2025
DEFINITION  M13 family metallopeptidase neprilysin 5 [Osmia lignaria lignaria].
ACCESSION   XP_034171908
VERSION     XP_034171908.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316017.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171908.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..760
                     /product="M13 family metallopeptidase neprilysin 5"
                     /calculated_mol_wt=87907
     Region          51..758
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(81,549,554..557,576,591..592,595..596,599,657,701,
                     704..705,721..722,728)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..760
                     /gene="Nep5"
                     /coded_by="XM_034316017.2:102..2384"
                     /db_xref="GeneID:117600489"
ORIGIN      
        1 mimlftlial lltmtlvfsi lyairkerql cetekcvria aslkesmdts vdpcddfyky
       61 vcgkwpdehp ipdnsltnsw feerrervyr kireslrenv trsnvpwavt qakilysscm
      121 dvhsmdelgv aplfdlleel nlpsipaaft kkttdyieqm arvkkvlgrd iffgfdiipd
      181 prntsnnimv ldtpitsspl pndkelerrl hsirsrfrkl eddevedelm selksaelky
      241 mteiikqvvn ngtldacsln qssfpeeedl eevveilyel sstfyylsrl ennqsiwdvd
      301 ptdgdymlvd dlqkltdeyv tevnstlapk plwkpfiesv fkdidtldln rkdkvligdl
      361 eylkevalll aasdeeelet yiwwvivdiv aphssstlrk iwinyvtemt sieigepksl
      421 lcasavndlm gmavswlfvd pqfhqdkgkk vfemmgdike afaslvlrtd wmdkqtkaat
      481 leknrkmesq igfpdwlfde rvlneyyegi dlseteylsn miqiirlmsi selecihdin
      541 ynnesywatd ptdvnafhty qfnhitipag ilqfpfyelg leslnygaig tvlghelthg
      601 fdnsgrhyds dgnvrqwwtn etilrynekt dcfvnhynny yevevddyid gertlgenia
      661 dngglreavi ayerwkarhg qepllpgfth ltqeqlvflg fahvwcesyt pkslkwilld
      721 shcpghvrlq gvlknskefs eawkcpvgsk mnppnkchvw
//
LOCUS       XP_034171923             532 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600490 [Osmia lignaria lignaria].
ACCESSION   XP_034171923
VERSION     XP_034171923.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316032.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171923.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..532
                     /product="uncharacterized protein LOC117600490"
                     /calculated_mol_wt=57600
     Region          <135..>290
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     CDS             1..532
                     /gene="LOC117600490"
                     /coded_by="XM_034316032.2:59..1657"
                     /db_xref="GeneID:117600490"
ORIGIN      
        1 mvhdssskhh tanmklllvi laclaassav pvekqkrdil pgdprygtdq hhhhhhhenv
       61 vedarassgy sysfpetenq gahahlpsns ygppgyqpgt plnkefvapt sdviqystpv
      121 pnfqvsdsvq tpqltntyip vntevkktvt tatisvpvek tnfvkaappa tsygtpvahh
      181 ttntldassd srvettlktv ktqlqdhqll dvpspqvtvt qqapvskgva hyatgvksip
      241 vtttftknlq adtlftdnlp tfqssiggfy napsyslptt vytnlqpsys tfhtgftglg
      301 vdyplrtlsh qvqfqqplta hfsnaiptsy sfpqtsyqlp qtltqfvqhs epsvstvqny
      361 spvqtqtkvq pvqqnletvt hvepahhkve tltpvhkvqs vevlpqrtvv esvktnatpl
      421 thtqvqkgpi gdffqnfsen lpslpsfpsl pslpslpsfp slpglptlss tpapqiesas
      481 pvstqvfesk dsiaienpll sadnnlkgtv vqhvtpaney vqptaanggy vy
//
LOCUS       XP_034172325             495 aa            linear   INV 03-SEP-2025
DEFINITION  motile sperm domain-containing protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172325
VERSION     XP_034172325.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="motile sperm domain-containing protein 2 isoform
                     X1"
                     /calculated_mol_wt=56562
     Region          89..230
                     /region_name="CRAL_TRIO"
                     /note="CRAL/TRIO domain; pfam00650"
                     /db_xref="CDD:459890"
     Region          284..390
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     CDS             1..495
                     /gene="LOC117600686"
                     /coded_by="XM_034316434.2:155..1642"
                     /db_xref="GeneID:117600686"
ORIGIN      
        1 mevqtelisd lrekflkkle degppdpkfh pvdiakvkds nswlkrfleh nenniqesln
       61 mlwetctwrs kfgtneitea nvkkeyiesg lcfnfgkdkd gkamlvikck lhtkgskdfn
      121 elkraivywf erlerqtngn qislffdmad tgisnmdmef tkyliglfks yypnflnyii
      181 ifempwilnt afklikswlp pkaipkikfv hkgniqefvd pndvltcwgg nndyvfkfvp
      241 eaqnhtdntl ngkldnkkvh faegsplteq sptsfgeqtt keqllsiepn aiifnktgnd
      301 iigtitlkni stekplsykv kttspekfrv rpssgvlspq eqrvisvvlq sgynlrgllh
      361 ndkflvmclp lkdanasnee laaiwksekp aeqhrlgccd ggyennevqk sqsilssgms
      421 enghidllyr kmthlketnv klhndvallk yslflsiivt iataiivvyi lkidiqnfmd
      481 qhshgqthhh ihngl
//
LOCUS       XP_034172330             435 aa            linear   INV 03-SEP-2025
DEFINITION  motile sperm domain-containing protein 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172330
VERSION     XP_034172330.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316439.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..435
                     /product="motile sperm domain-containing protein 2 isoform
                     X2"
                     /calculated_mol_wt=49489
     Region          29..170
                     /region_name="CRAL_TRIO"
                     /note="CRAL/TRIO domain; pfam00650"
                     /db_xref="CDD:459890"
     Region          224..330
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     CDS             1..435
                     /gene="LOC117600686"
                     /coded_by="XM_034316439.2:239..1546"
                     /db_xref="GeneID:117600686"
ORIGIN      
        1 mlwetctwrs kfgtneitea nvkkeyiesg lcfnfgkdkd gkamlvikck lhtkgskdfn
       61 elkraivywf erlerqtngn qislffdmad tgisnmdmef tkyliglfks yypnflnyii
      121 ifempwilnt afklikswlp pkaipkikfv hkgniqefvd pndvltcwgg nndyvfkfvp
      181 eaqnhtdntl ngkldnkkvh faegsplteq sptsfgeqtt keqllsiepn aiifnktgnd
      241 iigtitlkni stekplsykv kttspekfrv rpssgvlspq eqrvisvvlq sgynlrgllh
      301 ndkflvmclp lkdanasnee laaiwksekp aeqhrlgccd ggyennevqk sqsilssgms
      361 enghidllyr kmthlketnv klhndvallk yslflsiivt iataiivvyi lkidiqnfmd
      421 qhshgqthhh ihngl
//
LOCUS       XP_076546104             426 aa            linear   INV 03-SEP-2025
DEFINITION  motile sperm domain-containing protein 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546104
VERSION     XP_076546104.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689989.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="motile sperm domain-containing protein 2 isoform
                     X3"
                     /calculated_mol_wt=48430
     Region          20..161
                     /region_name="CRAL_TRIO"
                     /note="CRAL/TRIO domain; pfam00650"
                     /db_xref="CDD:459890"
     Region          215..321
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     CDS             1..426
                     /gene="LOC117600686"
                     /coded_by="XM_076689989.1:130..1410"
                     /db_xref="GeneID:117600686"
ORIGIN      
        1 myyliveite anvkkeyies glcfnfgkdk dgkamlvikc klhtkgskdf nelkraivyw
       61 ferlerqtng nqislffdma dtgisnmdme ftkyliglfk syypnflnyi iifempwiln
      121 tafklikswl ppkaipkikf vhkgniqefv dpndvltcwg gnndyvfkfv peaqnhtdnt
      181 lngkldnkkv hfaegsplte qsptsfgeqt tkeqllsiep naiifnktgn diigtitlkn
      241 istekplsyk vkttspekfr vrpssgvlsp qeqrvisvvl qsgynlrgll hndkflvmcl
      301 plkdanasne elaaiwksek paeqhrlgcc dggyennevq ksqsilssgm senghidlly
      361 rkmthlketn vklhndvall kyslflsiiv tiataiivvy ilkidiqnfm dqhshgqthh
      421 hihngl
//
LOCUS       XP_034176067            1127 aa            linear   INV 03-SEP-2025
DEFINITION  adenylyl cyclase X D isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034176067
VERSION     XP_034176067.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320176.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176067.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1127
                     /product="adenylyl cyclase X D isoform X1"
                     /calculated_mol_wt=129022
     Region          128..473
                     /region_name="AcyC"
                     /note="Adenylate cyclase, class 3 [Signal transduction
                     mechanisms]; COG2114"
                     /db_xref="CDD:441717"
     Region          304..488
                     /region_name="Nucleotidyl_cyc_III"
                     /note="Class III nucleotidyl cyclases; cl11967"
                     /db_xref="CDD:448371"
     Site            order(314,316..321,356,358..360,363,429..431,435..436,
                     439..440)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(316,360)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(321,333,336..337,340,344,356..357,417,420,430..433,
                     436)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          913..1108
                     /region_name="Nucleotidyl_cyc_III"
                     /note="Class III nucleotidyl cyclases; cl11967"
                     /db_xref="CDD:448371"
     Site            order(924,926..927,928,965,967..969,972,1051..1053,
                     1057..1058,1061..1062,1098)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(926,969)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(928,938,941..942,945,949,965..966,1039,1042,
                     1052..1055,1058,1098)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..1127
                     /gene="ACXD"
                     /coded_by="XM_034320176.2:107..3490"
                     /db_xref="GeneID:117602314"
ORIGIN      
        1 mefelvtras lnslqmvgdg rivspnslde wtwsnlrkqf kyksleklft vyqrrlqygy
       61 lslfvllqll lgityclili tmvsiencvp dvvvctvmsa llcpvialsf rskiisrnny
      121 lsvilscmiv vllvvgdlav plyytfkqad drlppirpay athallacyv flpltenlha
      181 filgvtatmc ylatlslity rntfdystki itdtiyficv nglglyfrfm nevvirrsfl
      241 drrkcvestl rlnyekdqee qltrsilpqh iaakikkdfr difkfieehk kpppkgrphh
      301 dlcvethnnv silyadvvnf sgltvtlpvr klvetlndlf gsfdeaserh nvlrikflgd
      361 cyycvsgvpt pnsqhakscv dlgldmikii kevrgrvrre cgvdvnmrig vhsgniisgi
      421 lgankwqydv wsrdvviank meqtgqpgkv hvtqqtldlv naseydcvpv dsindevlkk
      481 ygirsylitp slppdspttq nsentltvis pdsppvankh yvrfsnvrts nvsssgprns
      541 rrltttinnh vdvfagtyrr rthfmdsclr dyhlmlkaad aemenaikqm pltkweqwcr
      601 wehinplflt fqqwnweipf lkepdplfkf yvssavfvli fmgliylvpa isvsewhlst
      661 tisysvailf lialipivwm hfawnrykdp hdeheghvph prnkllcfly qtsvkvvwst
      721 flrtilylvm timlaacamf dmifecnnvd elasnnitss vaepgaskle cvatpwqmtq
      781 tcslailtsf lflrlhylik lvmgitvvaf yswnvwihrs nlfrsgeswn pyleprlahi
      841 lsivfltfsl hlidrqaeyl srldylwkrq ltkeqdeafh trnanklllr nilpehvaef
      901 ylnmnrteen epyheahnnv avmfasltdv sidesnvlid lneiicefdk llfepyfvcr
      961 iekikiagtt ymaacgleat rrdsmrsses eesfnenvvk vmaqfavemm avlsklngrs
     1021 fyrtkpyklr igishgevta gvvgaqkply diwgdavnma srmdttgvpg kiqvttdtaa
     1081 vleqqgvkch lrgntyvkpk gyvttyfvgi deerqlerms sveetsl
//
LOCUS       XP_076546105            1127 aa            linear   INV 03-SEP-2025
DEFINITION  adenylyl cyclase X D isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546105
VERSION     XP_076546105.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689990.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1127
                     /product="adenylyl cyclase X D isoform X1"
                     /calculated_mol_wt=129022
     Region          128..473
                     /region_name="AcyC"
                     /note="Adenylate cyclase, class 3 [Signal transduction
                     mechanisms]; COG2114"
                     /db_xref="CDD:441717"
     Region          304..488
                     /region_name="Nucleotidyl_cyc_III"
                     /note="Class III nucleotidyl cyclases; cl11967"
                     /db_xref="CDD:448371"
     Site            order(314,316..321,356,358..360,363,429..431,435..436,
                     439..440)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(316,360)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(321,333,336..337,340,344,356..357,417,420,430..433,
                     436)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          913..1108
                     /region_name="Nucleotidyl_cyc_III"
                     /note="Class III nucleotidyl cyclases; cl11967"
                     /db_xref="CDD:448371"
     Site            order(924,926..927,928,965,967..969,972,1051..1053,
                     1057..1058,1061..1062,1098)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(926,969)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(928,938,941..942,945,949,965..966,1039,1042,
                     1052..1055,1058,1098)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..1127
                     /gene="ACXD"
                     /coded_by="XM_076689990.1:21..3404"
                     /db_xref="GeneID:117602314"
ORIGIN      
        1 mefelvtras lnslqmvgdg rivspnslde wtwsnlrkqf kyksleklft vyqrrlqygy
       61 lslfvllqll lgityclili tmvsiencvp dvvvctvmsa llcpvialsf rskiisrnny
      121 lsvilscmiv vllvvgdlav plyytfkqad drlppirpay athallacyv flpltenlha
      181 filgvtatmc ylatlslity rntfdystki itdtiyficv nglglyfrfm nevvirrsfl
      241 drrkcvestl rlnyekdqee qltrsilpqh iaakikkdfr difkfieehk kpppkgrphh
      301 dlcvethnnv silyadvvnf sgltvtlpvr klvetlndlf gsfdeaserh nvlrikflgd
      361 cyycvsgvpt pnsqhakscv dlgldmikii kevrgrvrre cgvdvnmrig vhsgniisgi
      421 lgankwqydv wsrdvviank meqtgqpgkv hvtqqtldlv naseydcvpv dsindevlkk
      481 ygirsylitp slppdspttq nsentltvis pdsppvankh yvrfsnvrts nvsssgprns
      541 rrltttinnh vdvfagtyrr rthfmdsclr dyhlmlkaad aemenaikqm pltkweqwcr
      601 wehinplflt fqqwnweipf lkepdplfkf yvssavfvli fmgliylvpa isvsewhlst
      661 tisysvailf lialipivwm hfawnrykdp hdeheghvph prnkllcfly qtsvkvvwst
      721 flrtilylvm timlaacamf dmifecnnvd elasnnitss vaepgaskle cvatpwqmtq
      781 tcslailtsf lflrlhylik lvmgitvvaf yswnvwihrs nlfrsgeswn pyleprlahi
      841 lsivfltfsl hlidrqaeyl srldylwkrq ltkeqdeafh trnanklllr nilpehvaef
      901 ylnmnrteen epyheahnnv avmfasltdv sidesnvlid lneiicefdk llfepyfvcr
      961 iekikiagtt ymaacgleat rrdsmrsses eesfnenvvk vmaqfavemm avlsklngrs
     1021 fyrtkpyklr igishgevta gvvgaqkply diwgdavnma srmdttgvpg kiqvttdtaa
     1081 vleqqgvkch lrgntyvkpk gyvttyfvgi deerqlerms sveetsl
//
LOCUS       XP_034176078            1091 aa            linear   INV 03-SEP-2025
DEFINITION  adenylyl cyclase X D isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034176078
VERSION     XP_034176078.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320187.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176078.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1091
                     /product="adenylyl cyclase X D isoform X2"
                     /calculated_mol_wt=124918
     Region          92..437
                     /region_name="AcyC"
                     /note="Adenylate cyclase, class 3 [Signal transduction
                     mechanisms]; COG2114"
                     /db_xref="CDD:441717"
     Region          268..452
                     /region_name="Nucleotidyl_cyc_III"
                     /note="Class III nucleotidyl cyclases; cl11967"
                     /db_xref="CDD:448371"
     Site            order(278,280..285,320,322..324,327,393..395,399..400,
                     403..404)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(280,324)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(285,297,300..301,304,308,320..321,381,384,394..397,
                     400)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          877..1072
                     /region_name="Nucleotidyl_cyc_III"
                     /note="Class III nucleotidyl cyclases; cl11967"
                     /db_xref="CDD:448371"
     Site            order(888,890..891,892,929,931..933,936,1015..1017,
                     1021..1022,1025..1026,1062)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(890,933)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(892,902,905..906,909,913,929..930,1003,1006,
                     1016..1019,1022,1062)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..1091
                     /gene="ACXD"
                     /coded_by="XM_034320187.2:107..3382"
                     /db_xref="GeneID:117602314"
ORIGIN      
        1 mkqfkyksle klftvyqrrl qygylslfvl lqlllgityc lilitmvsie ncvpdvvvct
       61 vmsallcpvi alsfrskiis rnnylsvils cmivvllvvg dlavplyytf kqaddrlppi
      121 rpayathall acyvflplte nlhafilgvt atmcylatls lityrntfdy stkiitdtiy
      181 ficvnglgly frfmnevvir rsfldrrkcv estlrlnyek dqeeqltrsi lpqhiaakik
      241 kdfrdifkfi eehkkpppkg rphhdlcvet hnnvsilyad vvnfsgltvt lpvrklvetl
      301 ndlfgsfdea serhnvlrik flgdcyycvs gvptpnsqha kscvdlgldm ikiikevrgr
      361 vrrecgvdvn mrigvhsgni isgilgankw qydvwsrdvv iankmeqtgq pgkvhvtqqt
      421 ldlvnaseyd cvpvdsinde vlkkygirsy litpslppds pttqnsentl tvispdsppv
      481 ankhyvrfsn vrtsnvsssg prnsrrlttt innhvdvfag tyrrrthfmd sclrdyhlml
      541 kaadaemena ikqmpltkwe qwcrwehinp lfltfqqwnw eipflkepdp lfkfyvssav
      601 fvlifmgliy lvpaisvsew hlsttisysv ailflialip ivwmhfawnr ykdphdeheg
      661 hvphprnkll cflyqtsvkv vwstflrtil ylvmtimlaa camfdmifec nnvdelasnn
      721 itssvaepga sklecvatpw qmtqtcslai ltsflflrlh yliklvmgit vvafyswnvw
      781 ihrsnlfrsg eswnpylepr lahilsivfl tfslhlidrq aeylsrldyl wkrqltkeqd
      841 eafhtrnank lllrnilpeh vaefylnmnr teenepyhea hnnvavmfas ltdvsidesn
      901 vlidlneiic efdkllfepy fvcriekiki agttymaacg leatrrdsmr sseseesfne
      961 nvvkvmaqfa vemmavlskl ngrsfyrtkp yklrigishg evtagvvgaq kplydiwgda
     1021 vnmasrmdtt gvpgkiqvtt dtaavleqqg vkchlrgnty vkpkgyvtty fvgideerql
     1081 ermssveets l
//
LOCUS       XP_076546106            1082 aa            linear   INV 03-SEP-2025
DEFINITION  importin-4 [Osmia lignaria lignaria].
ACCESSION   XP_076546106
VERSION     XP_076546106.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689991.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1082
                     /product="importin-4"
                     /calculated_mol_wt=120985
     Region          39..>622
                     /region_name="KAP95"
                     /note="Karyopherin (importin) beta [Intracellular
                     trafficking and secretion]; COG5215"
                     /db_xref="CDD:227540"
     Site            order(325..326,329,332..333,364..365,368,371..372,375,
                     406..407,410,413..414,417,456..457,460,463..464,498..499,
                     502,505..506)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          343..375
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          388..418
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          429..464
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          477..506
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          859..892
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          861..968
                     /region_name="HEAT_2"
                     /note="HEAT repeats; pfam13646"
                     /db_xref="CDD:433376"
     Region          876..928
                     /region_name="HEAT_EZ"
                     /note="HEAT-like repeat; pfam13513"
                     /db_xref="CDD:463906"
     Region          901..927
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          938..970
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          982..1012
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1022..1050
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1082
                     /gene="LOC143305648"
                     /coded_by="XM_076689991.1:82..3330"
                     /db_xref="GeneID:143305648"
ORIGIN      
        1 meeillkllv adnatiqqgt telreafkkp estpalcqli vtstnsqirq yaavllrkry
       61 gkgkhwlklp qhirtefksv ilqalvneqe kfvknaiaql igvivkhelp tngwpevlqf
      121 vqqlvtsenl ndkelgtytl simteiapda ylthvgslav llaqtlnslq dlgnpvayyv
      181 lkimqnlvpl vegdqmmvna yhqmmprvmt tiqsltatne dkaiecfell delcenavsv
      241 iaphvkplvs mclaiasnks lddalrvkav sfigwlartk kkaiikhklv epiidllfnl
      301 mstrpeddny eiyfsgdned ntpvtcatqt ldllalnlpp eklipqllhy iepslqgtdv
      361 yakkasylam avlaegcsey irtkylesfl rctcqgitds ipvvrnaalf algqfsehlq
      421 pdisqyssel lpvlfeylgq icahikqekk eppsvdrmfy alemfcenln esllpylptl
      481 merlfeilsa dtpvhvrels lsaigsaama skenmlpyfe rivsildsyl sekqseetmc
      541 lqvqavdtlg viartigdkn faplagrsln fgmkllkete dpdlkksiyg lfasistimk
      601 keiaaalpei vdymitsiqs segivphfke detsafpvye dlsenenend eedientdne
      661 dddddddvag ysvenayiee keeailalke iaehteeafl pyleksfeet fklinypqed
      721 irkavidail qfcinfskin tnegkeallk alsvfipkls elirlgdert vaisgldayt
      781 ellkeiksdv lvgeghkdai mncvtdvmsg ktecqdqeea edvdteaeqd elliecagdv
      841 lsnfgkiipp edfalyfqtv lpmllerlkk nkseaqrsfa vgtisecfsg lkhtvaafip
      901 qllpiflrlt ddpsaevrnn aiygigelal hgkdavyshy pdilqvlsga iakeshagar
      961 dnvvgaiarl iianysnlpl dqvfpvfvnq lplkedfeen kavfrsiltl yqaghsilqp
     1021 hmqvllkvai svlhedratd deaknfvmef sksaqrdfpn ewnaayaelp vdvatkiqrt
     1081 fc
//
LOCUS       XP_076546107            1082 aa            linear   INV 03-SEP-2025
DEFINITION  importin-4 [Osmia lignaria lignaria].
ACCESSION   XP_076546107
VERSION     XP_076546107.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689992.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1082
                     /product="importin-4"
                     /calculated_mol_wt=120985
     Region          39..>622
                     /region_name="KAP95"
                     /note="Karyopherin (importin) beta [Intracellular
                     trafficking and secretion]; COG5215"
                     /db_xref="CDD:227540"
     Site            order(325..326,329,332..333,364..365,368,371..372,375,
                     406..407,410,413..414,417,456..457,460,463..464,498..499,
                     502,505..506)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          343..375
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          388..418
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          429..464
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          477..506
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          859..892
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          861..968
                     /region_name="HEAT_2"
                     /note="HEAT repeats; pfam13646"
                     /db_xref="CDD:433376"
     Region          876..928
                     /region_name="HEAT_EZ"
                     /note="HEAT-like repeat; pfam13513"
                     /db_xref="CDD:463906"
     Region          901..927
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          938..970
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          982..1012
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1022..1050
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1082
                     /gene="LOC143305648"
                     /coded_by="XM_076689992.1:146..3394"
                     /db_xref="GeneID:143305648"
ORIGIN      
        1 meeillkllv adnatiqqgt telreafkkp estpalcqli vtstnsqirq yaavllrkry
       61 gkgkhwlklp qhirtefksv ilqalvneqe kfvknaiaql igvivkhelp tngwpevlqf
      121 vqqlvtsenl ndkelgtytl simteiapda ylthvgslav llaqtlnslq dlgnpvayyv
      181 lkimqnlvpl vegdqmmvna yhqmmprvmt tiqsltatne dkaiecfell delcenavsv
      241 iaphvkplvs mclaiasnks lddalrvkav sfigwlartk kkaiikhklv epiidllfnl
      301 mstrpeddny eiyfsgdned ntpvtcatqt ldllalnlpp eklipqllhy iepslqgtdv
      361 yakkasylam avlaegcsey irtkylesfl rctcqgitds ipvvrnaalf algqfsehlq
      421 pdisqyssel lpvlfeylgq icahikqekk eppsvdrmfy alemfcenln esllpylptl
      481 merlfeilsa dtpvhvrels lsaigsaama skenmlpyfe rivsildsyl sekqseetmc
      541 lqvqavdtlg viartigdkn faplagrsln fgmkllkete dpdlkksiyg lfasistimk
      601 keiaaalpei vdymitsiqs segivphfke detsafpvye dlsenenend eedientdne
      661 dddddddvag ysvenayiee keeailalke iaehteeafl pyleksfeet fklinypqed
      721 irkavidail qfcinfskin tnegkeallk alsvfipkls elirlgdert vaisgldayt
      781 ellkeiksdv lvgeghkdai mncvtdvmsg ktecqdqeea edvdteaeqd elliecagdv
      841 lsnfgkiipp edfalyfqtv lpmllerlkk nkseaqrsfa vgtisecfsg lkhtvaafip
      901 qllpiflrlt ddpsaevrnn aiygigelal hgkdavyshy pdilqvlsga iakeshagar
      961 dnvvgaiarl iianysnlpl dqvfpvfvnq lplkedfeen kavfrsiltl yqaghsilqp
     1021 hmqvllkvai svlhedratd deaknfvmef sksaqrdfpn ewnaayaelp vdvatkiqrt
     1081 fc
//
LOCUS       XP_034176100             636 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional repressor p66-beta simjang isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034176100
VERSION     XP_034176100.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320209.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="transcriptional repressor p66-beta simjang
                     isoform X1"
                     /calculated_mol_wt=68965
     Region          126..165
                     /region_name="P66_CC"
                     /note="Coiled-coil and interaction region of P66A and P66B
                     with MBD2; pfam16563"
                     /db_xref="CDD:465178"
     Region          <163..>314
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..636
                     /gene="simj"
                     /coded_by="XM_034320209.2:586..2496"
                     /db_xref="GeneID:117602334"
ORIGIN      
        1 meamdldgda vvdlsvssgr rnspsitvns gdvggpldlg ihlsassnvd nnmpearniq
       61 naargilapk sgddrktrrn lrprieisya etpderring yvngnadsde gdmpplppik
      121 elssdelaer ertlrklree lrseemklvl lkklrqsqql keniaavpkv psklpppvpv
      181 qqaahslrta kapppllrgq papsrssslh apppgmllpp tvgrsptsst gmppnmvipq
      241 pphpsrgrpp satpnvsnyh apadrterst kdptpspahq vskllvgsqe nkttaslstp
      301 viseqerprd dnqtpaqrqa aaklalrkql ektllqippp kppppemhfv pnpsntefiy
      361 lvglehvvdf itkepaippp pepfectqck tdftpvwkwe kpvtggkkeg prgqhatfqr
      421 ppagrdprvi cehcvttnvk kalkaehtnr lktafvkalq qeqeieqrla qaacpspdpp
      481 apkpvpkaat ptrrvatppa pppqvppapt laptppapkl qehplvklae sgkfgphhaa
      541 aaaalqqqll relaknpvpg lpphqplpah mmppftsily pyqlamaqag gkglaelqrq
      601 aadlqrqyll dmipsqasqa qgnqapprah phnwkt
//
LOCUS       XP_034176126             633 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional repressor p66-beta simjang isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034176126
VERSION     XP_034176126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="transcriptional repressor p66-beta simjang
                     isoform X2"
                     /calculated_mol_wt=68651
     Region          <121..307
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     Region          126..165
                     /region_name="P66_CC"
                     /note="Coiled-coil and interaction region of P66A and P66B
                     with MBD2; pfam16563"
                     /db_xref="CDD:465178"
     CDS             1..633
                     /gene="simj"
                     /coded_by="XM_034320235.2:586..2487"
                     /db_xref="GeneID:117602334"
ORIGIN      
        1 meamdldgda vvdlsvssgr rnspsitvns gdvggpldlg ihlsassnvd nnmpearniq
       61 naargilapk sgddrktrrn lrprieisya etpderring yvngnadsde gdmpplppik
      121 elssdelaer ertlrklree lrseemklvl lkklrqsqql keniaavpkv psklpppvpv
      181 qqaahslrta kapppllrgq papsrssslh apppgmllpp tvgrsptsst gmppnmvipq
      241 pphpsrgrpp satpnvsnyh apadrterst kdptpspahq llvgsqenkt taslstpvis
      301 eqerprddnq tpaqrqaaak lalrkqlekt llqipppkpp ppemhfvpnp sntefiylvg
      361 lehvvdfitk epaippppep fectqcktdf tpvwkwekpv tggkkegprg qhatfqrppa
      421 grdprviceh cvttnvkkal kaehtnrlkt afvkalqqeq eieqrlaqaa cpspdppapk
      481 pvpkaatptr rvatppappp qvppaptlap tppapklqeh plvklaesgk fgphhaaaaa
      541 alqqqllrel aknpvpglpp hqplpahmmp pftsilypyq lamaqaggkg laelqrqaad
      601 lqrqylldmi psqasqaqgn qapprahphn wkt
//
LOCUS       XP_034176135             614 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional repressor p66-beta simjang isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034176135
VERSION     XP_034176135.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320244.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..614
                     /product="transcriptional repressor p66-beta simjang
                     isoform X3"
                     /calculated_mol_wt=66680
     Region          126..165
                     /region_name="P66_CC"
                     /note="Coiled-coil and interaction region of P66A and P66B
                     with MBD2; pfam16563"
                     /db_xref="CDD:465178"
     Region          <163..>292
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..614
                     /gene="simj"
                     /coded_by="XM_034320244.2:585..2429"
                     /db_xref="GeneID:117602334"
ORIGIN      
        1 meamdldgda vvdlsvssgr rnspsitvns gdvggpldlg ihlsassnvd nnmpearniq
       61 naargilapk sgddrktrrn lrprieisya etpderring yvngnadsde gdmpplppik
      121 elssdelaer ertlrklree lrseemklvl lkklrqsqql keniaavpkv psklpppvpv
      181 qqaahslrta kapppllrgq papsrssslh apppgmllpp tvgrsptsst gmppnmvipq
      241 pphpsrgrpp satpnvsnyh apadrterst kdptpspahq vskerprddn qtpaqrqaaa
      301 klalrkqlek tllqipppkp pppemhfvpn psntefiylv glehvvdfit kepaippppe
      361 pfectqcktd ftpvwkwekp vtggkkegpr gqhatfqrpp agrdprvice hcvttnvkka
      421 lkaehtnrlk tafvkalqqe qeieqrlaqa acpspdppap kpvpkaatpt rrvatppapp
      481 pqvppaptla ptppapklqe hplvklaesg kfgphhaaaa aalqqqllre laknpvpglp
      541 phqplpahmm ppftsilypy qlamaqaggk glaelqrqaa dlqrqylldm ipsqasqaqg
      601 nqapprahph nwkt
//
LOCUS       XP_076546108             584 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional repressor p66-beta simjang isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546108
VERSION     XP_076546108.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689993.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..584
                     /product="transcriptional repressor p66-beta simjang
                     isoform X4"
                     /calculated_mol_wt=63726
     Region          74..113
                     /region_name="P66_CC"
                     /note="Coiled-coil and interaction region of P66A and P66B
                     with MBD2; pfam16563"
                     /db_xref="CDD:465178"
     Region          <111..>262
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..584
                     /gene="simj"
                     /coded_by="XM_076689993.1:127..1881"
                     /db_xref="GeneID:117602334"
ORIGIN      
        1 mpearniqna argilapksg ddrktrrnlr prieisyaet pderringyv ngnadsdegd
       61 mpplppikel ssdelaerer tlrklreelr seemklvllk klrqsqqlke niaavpkvps
      121 klpppvpvqq aahslrtaka pppllrgqpa psrssslhap ppgmllpptv grsptsstgm
      181 ppnmvipqpp hpsrgrppsa tpnvsnyhap adrterstkd ptpspahqvs kllvgsqenk
      241 ttaslstpvi seqerprddn qtpaqrqaaa klalrkqlek tllqipppkp pppemhfvpn
      301 psntefiylv glehvvdfit kepaippppe pfectqcktd ftpvwkwekp vtggkkegpr
      361 gqhatfqrpp agrdprvice hcvttnvkka lkaehtnrlk tafvkalqqe qeieqrlaqa
      421 acpspdppap kpvpkaatpt rrvatppapp pqvppaptla ptppapklqe hplvklaesg
      481 kfgphhaaaa aalqqqllre laknpvpglp phqplpahmm ppftsilypy qlamaqaggk
      541 glaelqrqaa dlqrqylldm ipsqasqaqg nqapprahph nwkt
//
LOCUS       XP_034176205             510 aa            linear   INV 03-SEP-2025
DEFINITION  F-box only protein 33 [Osmia lignaria lignaria].
ACCESSION   XP_034176205
VERSION     XP_034176205.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320314.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="F-box only protein 33"
                     /calculated_mol_wt=58423
     Region          65..112
                     /region_name="F-box_FBXO33"
                     /note="F-box domain found in F-box only protein 33
                     (FBXO33) and similar proteins; cd22104"
                     /db_xref="CDD:438876"
     Site            order(68,72,75..76,79..80,84,86..87,91..93,95)
                     /site_type="other"
                     /note="putative Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438876"
     CDS             1..510
                     /gene="LOC117602380"
                     /coded_by="XM_034320314.2:172..1704"
                     /db_xref="GeneID:117602380"
ORIGIN      
        1 miraengdvv yleqlknsve sdatekatsr kcegqlssvk anscssykga nrttemaqed
       61 nnpcwdnlps vilqeifsyl phncrlrasq vcrnwrytlf hprfwrkitf alkdedsilw
      121 sqclgenfgl svrevtircd tpnhsveeal sllkklscnr qlrklfless sssfeydddn
      181 ddspgyttpl ikslvkiiet snrlealslg cieeltasaa vlleplrlhh akhlttlsla
      241 svrdepddyd flqfdyefvf ssfvrlsilt ldyefitdyl lqaldngtme rlvihvhgwn
      301 dhylgttdsa wqnfiqknpk celrlnlihs yvgiqmldtd ilrpsmplth lkvlfcenvn
      361 iralhrlssw ysltlksliw idsmepritf epathdpddp dspdplvlva wkctklveiv
      421 fighkyyqen llaiarlrgc slkslvfaqr yiasdneswh maetitheie eimgrrwkll
      481 sdadlppvvn nafggdsrev implvlcdqk
//
LOCUS       XP_076546109             481 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602386 [Osmia lignaria lignaria].
ACCESSION   XP_076546109
VERSION     XP_076546109.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689994.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="uncharacterized protein LOC117602386"
                     /calculated_mol_wt=52583
     Region          <2..>168
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          84..161
                     /region_name="FH_FOXF"
                     /note="Forkhead (FH) domain found in the Forkhead box
                     protein F (FOXF) subfamily; cd20020"
                     /db_xref="CDD:410794"
     Site            order(88,106..107,110,129..130,132..133,136,143,153..155,
                     157)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410794"
     CDS             1..481
                     /gene="LOC117602386"
                     /coded_by="XM_076689994.1:1..1446"
                     /db_xref="GeneID:117602386"
ORIGIN      
        1 mdqtvivpst prgtsvamkn esetslhlqd qstvahansn svvprtslsm tsnsnqeqsl
       61 dplmcnpsst elprkpgarr qekppysyia livmaiqssp gkrltlseiy sflqqhfpff
      121 rgayqgwkns vrhnlslnec fiklpkglgr pgkghywtid psteymfeeg sfrrrprgfr
      181 rkcqalkpqy pqyfagsgpv gvqpsgyenl tpgsmeyang yqnqyqnyqe yamyapgapv
      241 sadwaypeat yktppiaevt ykttevtykt gepsvyrnge ivafksepgy asrnqdqlay
      301 kaategfsvk dhqhhadsvy kendtmmtyk cpsnpvpttq atgqdyyvgy glagvnntgn
      361 nvnvamqgip eqtgnsspvg nvssphsgcq tpvtdngikm qcpnsnsnss ggglidrkps
      421 yfghpagsvt lsslsslssl nlsnigglsi snipgtvssn ihhttttppt tmyydqikys
      481 m
//
LOCUS       XP_034176242             426 aa            linear   INV 03-SEP-2025
DEFINITION  flotillin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034176242
VERSION     XP_034176242.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320351.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="flotillin-1"
                     /calculated_mol_wt=47046
     Region          5..425
                     /region_name="YqiK"
                     /note="Uncharacterized membrane protein YqiK, contains
                     Band7/PHB/SPFH domain [Function unknown]; COG2268"
                     /db_xref="CDD:441869"
     CDS             1..426
                     /gene="LOC117602391"
                     /coded_by="XM_034320351.2:111..1391"
                     /db_xref="GeneID:117602391"
ORIGIN      
        1 mscgfvtcgp nealvvsgcc yskpllvpgg rvfvwpivqq vqkislntmt lqvesptvyt
       61 cqgvpisvtg iaqvkiqgqn eemlstaceq flgksedeih nialvtlegh qraimgsmtv
      121 eeiykdrkkf skevfevass dlvnmgitvv sytlkdirde egylkalgma rtaevkrdar
      181 igeaearrda qireaiaeeq rmaarflndt diakaqrdfe lkkaaydvev qtkkaeaema
      241 felqaaktkq rimeeqmqvk vvergqeiav qeqemlrrer eldatvrrpa naekyrlekm
      301 aeankmrlvm eaeaeaeair irgeaeafai eakakadaeq makkaaawne yksaamidmm
      361 ldtlpkvaae vaaplsqakk itmvssgngt vgaeklteev fnivtrvpel vknltgvdia
      421 ksvhaa
//
LOCUS       XP_034176256             422 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602401 [Osmia lignaria lignaria].
ACCESSION   XP_034176256
VERSION     XP_034176256.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320365.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="uncharacterized protein LOC117602401"
                     /calculated_mol_wt=48683
     Region          49..336
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..422
                     /gene="LOC117602401"
                     /coded_by="XM_034320365.2:217..1485"
                     /db_xref="GeneID:117602401"
ORIGIN      
        1 msntskdygk lkdvsshfte etlknilctv hngtevnvls wnfgqasakg dnylstvnki
       61 kvngivdgke iqvslvvksl pqnigrrnty rsvdffhnei ifytqvlpkl eefvrdkeqs
      121 qilcvprhfv svmdgkndyi tledvttlgy ksidrqncld qnqcmmilkt iakfhatsfa
      181 ckdqrkeefm kaienldety fsmnhwdwyk rfyerlvgia knalameypg seaekkfnsy
      241 kfdelfekaa efcnrkyhat sviiqgdcwv pnfmtrktne nealmldfql trcaspvldi
      301 stfiyactdk ivwktqfdel lkfyhnelck tikllgsnpe niypwstfme evkeqflfgl
      361 vfateiipms lldesdifdl daikddtavd iadiwtlsni ksksgrlrla dvivhavqkg
      421 fl
//
LOCUS       XP_034176612             308 aa            linear   INV 03-SEP-2025
DEFINITION  ADP,ATP carrier protein-like [Osmia lignaria lignaria].
ACCESSION   XP_034176612
VERSION     XP_034176612.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320721.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="ADP,ATP carrier protein-like"
                     /calculated_mol_wt=33935
     Region          1..295
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..308
                     /gene="LOC117602538"
                     /coded_by="XM_034320721.2:170..1096"
                     /db_xref="GeneID:117602538"
ORIGIN      
        1 mdayksflvd flaggisaav sktavapier vklllqvqht skqirpedry kgmidafvri
       61 pketgflsyw rgnlanviry fptqalnfaf kdkfktlfle gvpkdafwkq fagnlasgga
      121 agatsllfvy pldfartrla adigkadkre fkglgdclvk vfktdglfgl yrgfsvsvqg
      181 iiiyraayfg fydttrgllp dakntplyin fliaqvvttt agiisypfdt vrrrmmmqsg
      241 rskteimykn tldcwvktak aegvaaffkg afsnilrgtg galvltlyds vkdlidaalk
      301 kgstkpse
//
LOCUS       XP_034176402             295 aa            linear   INV 03-SEP-2025
DEFINITION  splicing factor C9orf78 [Osmia lignaria lignaria].
ACCESSION   XP_034176402
VERSION     XP_034176402.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320511.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="splicing factor C9orf78"
                     /calculated_mol_wt=33896
     Region          109..211
                     /region_name="Hep_59"
                     /note="Hepatocellular carcinoma-associated antigen 59;
                     pfam07052"
                     /db_xref="CDD:462074"
     CDS             1..295
                     /gene="LOC117602457"
                     /coded_by="XM_034320511.2:157..1044"
                     /db_xref="GeneID:117602457"
ORIGIN      
        1 mtsteehkie fkkkpkkqlr krhvssdedd eneeasvrek veemkiiqkl rerpkgvnvv
       61 glalgenvtp dvmtsdpfnv ktggmvnmaa lkntklkqnd ayetgigtqf naetnkrded
      121 eemvkyieee lskrkskned ktesgsnndk gsycspeeaa lqavpehlrq ssahrseeml
      181 snqmlsgipe vdlgieakir nieateeakl kllwdrhrkk dgpsqfvptn mavnfvqhnr
      241 fniedtdfqk skqdsddkkk vavprddfkg krkdngekat ddyhyerfkk qfrrf
//
LOCUS       XP_034176423             271 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin 7 [Osmia lignaria lignaria].
ACCESSION   XP_034176423
VERSION     XP_034176423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..271
                     /product="syntaxin 7"
                     /calculated_mol_wt=30332
     Region          26..132
                     /region_name="Syntaxin_2"
                     /note="Syntaxin-like protein; pfam14523"
                     /db_xref="CDD:464199"
     Region          178..237
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(206,213,216)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277192"
     Site            209
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277192"
     CDS             1..271
                     /gene="Syx7"
                     /coded_by="XM_034320532.2:270..1085"
                     /db_xref="GeneID:117602471"
ORIGIN      
        1 mdigfssyhn ggpareqdfg rlsqtigtsi lkisqnvssm qkmvnqlgss tdsqelrnql
       61 hqiqhytqql akdtsvllrd lailannsgs tspgeqrqrk mqrerlqdef tsalnsfqav
      121 qrlaaskeke mvrkakanag iapfgekkqe tlielqdsrt qkqtqqqqlk eeqnlrmlee
      181 qeasirqles nisdinqifk dlgalvydqg evidsieasv ertevsvnea tshvrqasmy
      241 qnklrkkkcv lviigvvvls iligiiawqs s
//
LOCUS       XP_034176548             194 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein CG1161 [Osmia lignaria lignaria].
ACCESSION   XP_034176548
VERSION     XP_034176548.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320657.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..194
                     /product="uncharacterized protein CG1161"
                     /calculated_mol_wt=21925
     Region          48..193
                     /region_name="Tmemb_9"
                     /note="TMEM9; pfam05434"
                     /db_xref="CDD:428471"
     CDS             1..194
                     /gene="LOC117602512"
                     /coded_by="XM_034320657.2:168..752"
                     /db_xref="GeneID:117602512"
ORIGIN      
        1 mlthiflvta vtllclsqse aqyddkrckc icpnvssvvn taqsslvrkt yiinvppsqc
       61 ncegvvlpkv gdqlkgkeei fcsrcgckye nrnttiikiv vilviwvisl lviymlflic
      121 ldpllnktih ktstnigyhe hnnvdddssi gpgtshpmge rgnvlnrvgh qqdkwkrqvr
      181 eqrrniydrh tmln
//
LOCUS       XP_034176562             185 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602519 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176562
VERSION     XP_034176562.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320671.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..185
                     /product="uncharacterized protein LOC117602519 isoform X1"
                     /calculated_mol_wt=20688
     Region          <107..172
                     /region_name="DUF1180"
                     /note="Protein of unknown function (DUF1180); pfam06679"
                     /db_xref="CDD:429067"
     CDS             1..185
                     /gene="LOC117602519"
                     /coded_by="XM_034320671.2:248..805"
                     /db_xref="GeneID:117602519"
ORIGIN      
        1 mtssriqiik yfiflsifll aqsavcdgnf rvnrtvpdgt vkkdnahnsl ekqkvpqqha
       61 ttidegnktv ndttlkadvn ktntqvtgsr kehvgeghtt slnvgalkrg fyvfvglsvl
      121 vmayivfrsf rlsktraqmv rkygvlthrq dvemrplpls eeddedttvf dasnvmtnnv
      181 qhqnl
//
LOCUS       XP_034176570             171 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602519 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176570
VERSION     XP_034176570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320679.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="uncharacterized protein LOC117602519 isoform X2"
                     /calculated_mol_wt=18948
     Region          <93..158
                     /region_name="DUF1180"
                     /note="Protein of unknown function (DUF1180); pfam06679"
                     /db_xref="CDD:429067"
     CDS             1..171
                     /gene="LOC117602519"
                     /coded_by="XM_034320679.2:16..531"
                     /db_xref="GeneID:117602519"
ORIGIN      
        1 msnintsqsa vcdgnfrvnr tvpdgtvkkd nahnslekqk vpqqhattid egnktvndtt
       61 lkadvnktnt qvtgsrkehv geghttslnv galkrgfyvf vglsvlvmay ivfrsfrlsk
      121 traqmvrkyg vlthrqdvem rplplseedd edttvfdasn vmtnnvqhqn l
//
LOCUS       XP_034176582             151 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602530 [Osmia lignaria lignaria].
ACCESSION   XP_034176582
VERSION     XP_034176582.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320691.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="uncharacterized protein LOC117602530"
                     /calculated_mol_wt=16781
     CDS             1..151
                     /gene="LOC117602530"
                     /coded_by="XM_034320691.2:120..575"
                     /db_xref="GeneID:117602530"
ORIGIN      
        1 mcsrvslllf sflllftgyr ceenvtsvte enalaklvkm fskpgafkri nailgensie
       61 esftscpkge eglecrraea rrsvdcpqgd cycyncaaag pelwasccre slrccshlaa
      121 acrtcdhpsl ypfcskhfkk cliepnekkq n
//
LOCUS       XP_034178542            3244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603465 [Osmia lignaria lignaria].
ACCESSION   XP_034178542
VERSION     XP_034178542.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322651.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178542.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3244
                     /product="uncharacterized protein LOC117603465"
                     /calculated_mol_wt=366051
     Region          <1806..2099
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..3244
                     /gene="LOC117603465"
                     /coded_by="XM_034322651.2:215..9949"
                     /db_xref="GeneID:117603465"
ORIGIN      
        1 mnyggnmpdw hqyntsqtne vapptqnvns ghlafipgvn hptlnalsls seglnkhqnd
       61 anfnprnyqs ynnvsngpni pnnsplasmv qmqncighyg ststrnpmld ninasvdhrn
      121 atvaainddi gyrtnqvpfn gpighlsgps cnlnsssgpg pgsgfgprtn sgpgsnsgtn
      181 sgngtgpnpm fgpgprhgpv pgpgsgvgsg nlgprnanig svssgkpgps sfmsckgtcc
      241 nsdpninyqq wekfgpyqnn tsfrdnvhps gcqmenrhfg nncnfrkdnl eakevmgpvl
      301 snapaidhrr nytdykyhkd hlvhrnypas sgmfhnypvq nynystehkk ynypmkehpk
      361 tnnmnisnsg mlkhqeqnfi nqqkfnnkqf qyqngnmlpk gvptlnvnan masspqnpyf
      421 ntqfsrnitt emnhecqett dsaaivnrmp stfmhsspsq hqvyqhkiai qkysmenhlr
      481 elsripgyqs hpkykecvhr yreilklqqs tsyqnpvqqa srvttavnas vtpinlqfdq
      541 ngllintsyi pegfpkvhha pnieqpseni ekqtkeqtia iatekcqqaq qpeqliipsq
      601 nehipspcvd tfqkqnqfsi ykdfnqnqlk vqtsesdnfn tlnpvksnet ammqqqkask
      661 efankpeldv rqflanwdet ddedastsnl qdsvlsettp vvvvsyenvd lssktaqgte
      721 aqvqkrtsyc snetgldtvk esetnviaaq dcltisyspt dsteiaktsk rtiregvvkp
      781 gsiihcisng pdeiptihiv dnleisnilg tcneqviqtl ekqktisffk etsnneteam
      841 nledtdqqdk netdvlstsy egslndvtkt tniedsdhvq aatvsinnqe lrglststks
      901 nmdlkkqnsf vseeshnhdd islpdlptse ctpisttlnt pthsdteess ehledlsist
      961 hlievmqnsp visftqlnse ekskneslgt lklkfrkens ykaneqevtl dnfdfsagns
     1021 kvrsvvsagt nanlsptesd detrasrrkk vktssvstst shskidlcar kraketmdle
     1081 dkgmnkyser slvskkdtdh srqskssetr rfddatsshn eenslchits qykhysvavi
     1141 kdnvslsdkr sarstdrtsg dksaelqtrt sndtkehdsv qfqssrksel lqkdlkpvdg
     1201 dvrytaeeir mlkeyrrkke klhqaqdksk hhshktveer spkndenssh prnktnells
     1261 vvnlsakkld areettghac sksfrknadf giqvanvnlk ikdsdigkel ileekgqeqm
     1321 kdslegikie invsckdqnl kgqeqnkrll yddgdlyeid eppaytsntl dcqcrlhake
     1381 qendsktidk ahraaekavd taevpstsat dtnagtnlkk rrrssmgtne etcvltsdsk
     1441 sshnnasckd tsiegtvcpv ddmdtvklnc aisntaivda nnkikakskl evgdtekdnn
     1501 nrsletmkvq kpattshvdt statdvlkrh eekemaskna kklpdnspev nakslsslna
     1561 idfnfdyrky shvdeldddy pgkwkkpkid qifedcdmfq sssgyanpif ssidkmedln
     1621 tvpvyttkdg kisyspnrrf tyhelmmear rreacfsgkk shyaetwnny ygskfrklyk
     1681 rkrhhpfner kkhefkntky lydrkrnysd efygrgqtky kdyvhsiknn nadwldhykm
     1741 dkmysssdsd eeivncnksr iaeaskrshe tkiesskspg nhvtidaldl eckekedtts
     1801 daclskdkdt slkdtqpvlp pdddkgtlvt vtedqenenv kstldecsvl nkscdlppek
     1861 lesnesenld eerieqhdks kdsiisgnee enintadgss pvkslnevsl ftenkqtsvs
     1921 stvkeeknat didkekeetg ddtkvlddqv sladdskniq ieicrnmqak psesleletd
     1981 vmdcssvkel sefhadsdnv aeqvsqesle sekyinvsne iaeessenik dkvdeensvv
     2041 ceenvlecke anvhlteeiv tlikereesl neeikkstet lvdendsnsp kevenensed
     2101 rkksesqida ttdqqnnsey tlletvenqk dtiiipeqld neelmlmdfs ketvyneddn
     2161 aaeslvssnt kkiqitlsni netpieeqmt iddlkdryne trlqelqnis vhnssrnssv
     2221 ekevpvlney ctiknsenni drtkededng kpdhssffnp ddssnhsses nmdvkaipkl
     2281 iikktdasnp kseslsncle fdncteryda kllsetrpki pkmiivknrs rsatpivell
     2341 ekgkserths sedndvsinn sdseqytlky ndytskvpkv kikledissk dlklylkrka
     2401 lskksvpkvk ikklktqdtk ahasvskfet edtsetdaid sndeenvfhe smisdaesek
     2461 ipklrlkrqd eekspdrtke ndsnaskvtp kkskktreed tkhsvkydni ttnddelrkk
     2521 fpqyivekip kviikrtqig tefkceiskg kktsiaetsk wqpkvklqrl elldhivkdl
     2581 kqskitlrni fntptritds nindtdnrds sqgnkvklsr snsasnlstv kckqrrlsdf
     2641 dyskmdmdnr vaglksvdse niglkrkskk klfsqskqid pylnsenene adeitsenml
     2701 rdsfrnllmd eyndlmtirs efsdfttees tpfignkfle sdnaqsynyd tndnsiikve
     2761 ssdesqttie ilpaspdscd getdknsefe nidrlhigda mptqlelele lmdnnmqhsd
     2821 vfndhashsr gkcankkstk iyfsefqrss gntfnrdmfk qteqssents sdyfycndll
     2881 vkevlaakea lkkclarpen qdlenrksrp ktvaekkqgl sfdfkgfdes rksenadcnd
     2941 gndnvtihrk siilekkeyl rknkhlsmek etasvnsqne sptastcgyt nvpesttisl
     3001 kaskkidens aneisvstea dvskladepg nagdvkdlhg eksecqkplq cqkddgsnda
     3061 kikednmpll vpefslnfds ssdrdssrsp pvitnqeevd naavseksie nkvitpieki
     3121 eeskehsykd cemtiadiit elayhekati khkrycnlce rwfptasrhr rhlagyqhry
     3181 meltqrksih alfilftgkp cprllpanvi rndcsigelt plqiavqdia kcveytqqnl
     3241 ktkd
//
LOCUS       XP_076546110            3244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603465 [Osmia lignaria lignaria].
ACCESSION   XP_076546110
VERSION     XP_076546110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689995.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3244
                     /product="uncharacterized protein LOC117603465"
                     /calculated_mol_wt=366051
     Region          <1806..2099
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..3244
                     /gene="LOC117603465"
                     /coded_by="XM_076689995.1:224..9958"
                     /db_xref="GeneID:117603465"
ORIGIN      
        1 mnyggnmpdw hqyntsqtne vapptqnvns ghlafipgvn hptlnalsls seglnkhqnd
       61 anfnprnyqs ynnvsngpni pnnsplasmv qmqncighyg ststrnpmld ninasvdhrn
      121 atvaainddi gyrtnqvpfn gpighlsgps cnlnsssgpg pgsgfgprtn sgpgsnsgtn
      181 sgngtgpnpm fgpgprhgpv pgpgsgvgsg nlgprnanig svssgkpgps sfmsckgtcc
      241 nsdpninyqq wekfgpyqnn tsfrdnvhps gcqmenrhfg nncnfrkdnl eakevmgpvl
      301 snapaidhrr nytdykyhkd hlvhrnypas sgmfhnypvq nynystehkk ynypmkehpk
      361 tnnmnisnsg mlkhqeqnfi nqqkfnnkqf qyqngnmlpk gvptlnvnan masspqnpyf
      421 ntqfsrnitt emnhecqett dsaaivnrmp stfmhsspsq hqvyqhkiai qkysmenhlr
      481 elsripgyqs hpkykecvhr yreilklqqs tsyqnpvqqa srvttavnas vtpinlqfdq
      541 ngllintsyi pegfpkvhha pnieqpseni ekqtkeqtia iatekcqqaq qpeqliipsq
      601 nehipspcvd tfqkqnqfsi ykdfnqnqlk vqtsesdnfn tlnpvksnet ammqqqkask
      661 efankpeldv rqflanwdet ddedastsnl qdsvlsettp vvvvsyenvd lssktaqgte
      721 aqvqkrtsyc snetgldtvk esetnviaaq dcltisyspt dsteiaktsk rtiregvvkp
      781 gsiihcisng pdeiptihiv dnleisnilg tcneqviqtl ekqktisffk etsnneteam
      841 nledtdqqdk netdvlstsy egslndvtkt tniedsdhvq aatvsinnqe lrglststks
      901 nmdlkkqnsf vseeshnhdd islpdlptse ctpisttlnt pthsdteess ehledlsist
      961 hlievmqnsp visftqlnse ekskneslgt lklkfrkens ykaneqevtl dnfdfsagns
     1021 kvrsvvsagt nanlsptesd detrasrrkk vktssvstst shskidlcar kraketmdle
     1081 dkgmnkyser slvskkdtdh srqskssetr rfddatsshn eenslchits qykhysvavi
     1141 kdnvslsdkr sarstdrtsg dksaelqtrt sndtkehdsv qfqssrksel lqkdlkpvdg
     1201 dvrytaeeir mlkeyrrkke klhqaqdksk hhshktveer spkndenssh prnktnells
     1261 vvnlsakkld areettghac sksfrknadf giqvanvnlk ikdsdigkel ileekgqeqm
     1321 kdslegikie invsckdqnl kgqeqnkrll yddgdlyeid eppaytsntl dcqcrlhake
     1381 qendsktidk ahraaekavd taevpstsat dtnagtnlkk rrrssmgtne etcvltsdsk
     1441 sshnnasckd tsiegtvcpv ddmdtvklnc aisntaivda nnkikakskl evgdtekdnn
     1501 nrsletmkvq kpattshvdt statdvlkrh eekemaskna kklpdnspev nakslsslna
     1561 idfnfdyrky shvdeldddy pgkwkkpkid qifedcdmfq sssgyanpif ssidkmedln
     1621 tvpvyttkdg kisyspnrrf tyhelmmear rreacfsgkk shyaetwnny ygskfrklyk
     1681 rkrhhpfner kkhefkntky lydrkrnysd efygrgqtky kdyvhsiknn nadwldhykm
     1741 dkmysssdsd eeivncnksr iaeaskrshe tkiesskspg nhvtidaldl eckekedtts
     1801 daclskdkdt slkdtqpvlp pdddkgtlvt vtedqenenv kstldecsvl nkscdlppek
     1861 lesnesenld eerieqhdks kdsiisgnee enintadgss pvkslnevsl ftenkqtsvs
     1921 stvkeeknat didkekeetg ddtkvlddqv sladdskniq ieicrnmqak psesleletd
     1981 vmdcssvkel sefhadsdnv aeqvsqesle sekyinvsne iaeessenik dkvdeensvv
     2041 ceenvlecke anvhlteeiv tlikereesl neeikkstet lvdendsnsp kevenensed
     2101 rkksesqida ttdqqnnsey tlletvenqk dtiiipeqld neelmlmdfs ketvyneddn
     2161 aaeslvssnt kkiqitlsni netpieeqmt iddlkdryne trlqelqnis vhnssrnssv
     2221 ekevpvlney ctiknsenni drtkededng kpdhssffnp ddssnhsses nmdvkaipkl
     2281 iikktdasnp kseslsncle fdncteryda kllsetrpki pkmiivknrs rsatpivell
     2341 ekgkserths sedndvsinn sdseqytlky ndytskvpkv kikledissk dlklylkrka
     2401 lskksvpkvk ikklktqdtk ahasvskfet edtsetdaid sndeenvfhe smisdaesek
     2461 ipklrlkrqd eekspdrtke ndsnaskvtp kkskktreed tkhsvkydni ttnddelrkk
     2521 fpqyivekip kviikrtqig tefkceiskg kktsiaetsk wqpkvklqrl elldhivkdl
     2581 kqskitlrni fntptritds nindtdnrds sqgnkvklsr snsasnlstv kckqrrlsdf
     2641 dyskmdmdnr vaglksvdse niglkrkskk klfsqskqid pylnsenene adeitsenml
     2701 rdsfrnllmd eyndlmtirs efsdfttees tpfignkfle sdnaqsynyd tndnsiikve
     2761 ssdesqttie ilpaspdscd getdknsefe nidrlhigda mptqlelele lmdnnmqhsd
     2821 vfndhashsr gkcankkstk iyfsefqrss gntfnrdmfk qteqssents sdyfycndll
     2881 vkevlaakea lkkclarpen qdlenrksrp ktvaekkqgl sfdfkgfdes rksenadcnd
     2941 gndnvtihrk siilekkeyl rknkhlsmek etasvnsqne sptastcgyt nvpesttisl
     3001 kaskkidens aneisvstea dvskladepg nagdvkdlhg eksecqkplq cqkddgsnda
     3061 kikednmpll vpefslnfds ssdrdssrsp pvitnqeevd naavseksie nkvitpieki
     3121 eeskehsykd cemtiadiit elayhekati khkrycnlce rwfptasrhr rhlagyqhry
     3181 meltqrksih alfilftgkp cprllpanvi rndcsigelt plqiavqdia kcveytqqnl
     3241 ktkd
//
LOCUS       XP_034178573            2393 aa            linear   INV 03-SEP-2025
DEFINITION  fatty acid synthase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034178573
VERSION     XP_034178573.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322682.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178573.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2393
                     /product="fatty acid synthase 1"
                     /calculated_mol_wt=262524
     Region          27..>844
                     /region_name="PksD"
                     /note="Acyl transferase domain in polyketide synthase
                     (PKS) enzymes [Secondary metabolites biosynthesis,
                     transport and catabolism]; COG3321"
                     /db_xref="CDD:442550"
     Region          877..1081
                     /region_name="hot_dog"
                     /note="The hotdog fold was initially identified in the E.
                     coli FabA (beta-hydroxydecanoyl-acyl carrier protein
                     (ACP)-dehydratase) structure and subsequently in 4HBT
                     (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A
                     number of other seemingly unrelated...; cl00509"
                     /db_xref="CDD:469797"
     Region          1259..>1422
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Region          1469..1757
                     /region_name="PKS_ER"
                     /note="Enoylreductase; smart00829"
                     /db_xref="CDD:214840"
     Site            order(1476,1557,1578,1580..1583,1602..1603,1607,
                     1626..1627,1650,1672,1697..1699,1746..1747,1749,1751)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176179"
     Region          <1778..2014
                     /region_name="KR_1_FAS_SDR_x"
                     /note="beta-ketoacyl reductase (KR) domain of fatty acid
                     synthase (FAS), subgroup 1, complex (x) SDRs; cd08954"
                     /db_xref="CDD:187657"
     Site            order(1795..1796,1798,1818..1821,1846..1849,1873..1875,
                     1896..1897,1921..1922,1936,1963..1964)
                     /site_type="other"
                     /note="putative NADP binding site [chemical binding]"
                     /db_xref="CDD:187657"
     Site            order(1897,1923,1936,1940)
                     /site_type="active"
                     /db_xref="CDD:187657"
     Region          2028..2080
                     /region_name="PKS_PP"
                     /note="Phosphopantetheine attachment site; smart00823"
                     /db_xref="CDD:214834"
     Region          <2114..2392
                     /region_name="EntF2"
                     /note="Thioesterase domain of type I polyketide synthase
                     or non-ribosomal peptide synthetase [Secondary metabolites
                     biosynthesis, transport and catabolism]; COG3319"
                     /db_xref="CDD:442548"
     CDS             1..2393
                     /gene="FASN1"
                     /coded_by="XM_034322682.2:428..7609"
                     /db_xref="GeneID:117603474"
ORIGIN      
        1 mpaqfesans pitreptmmn gdspystged vvitgfsgrf pesvdvdefr knlfegidmv
       61 tdderrwpsg myglptrtgk lkdlksfdat ffgvhakqan vmdpqlrmll eatyeaivda
      121 ginpndirgs ktgvfigvss sesdeywtgd pelvngyglt gccramfsnr isytfdftgp
      181 syaldtacss slyamhqaia airagecdsa ivggcnlllk ptaslqfhrl smlspdgtcr
      241 afdadgkgyv rseavsvvfl qksknarrvy atvvhsktnt dgnkvqgitf psgdmqnklm
      301 revyaeagvn padvvyveah gtgtkvgdpq einsiaklfc kgrktplllg siksnmghae
      361 pasgvssmak lfiametgvi pgnlhfktpn qdipslsdgs iqvidkntpw kgglvainsf
      421 gfgganahil lrsnpkpkls pvldsklpkl vtvsgrteea vevllekake yeyddefval
      481 lhdiyansvt ghlyrgysvm gnvntqevns cvgekrpiwf afsgmgsqwp gmgrellsie
      541 tfqrslhrca dalrpegidl mhliqhgtte tfanvvhsfv siaaiqvalv dllahlgiqp
      601 dgiighsvge lgcayadgtf tpeqtvlaay wrgrmildsk lepggmaavg lsweetkarc
      661 ppnimaacnn sadsvsisgp vedvlkfaae lkqqnifakv vdssgvafhs kyvapagpkl
      721 rtvlekvipn pkqrtarwis tsipeeawgt plaqlsspay hvnnllspvl fhqglahvpe
      781 naivieiaph cllqavlrrs lpptvtnigl hkrdhannig fflenigkly magaqpkvsk
      841 lyppvsypvg rgtpminslv kwdhstewsi adfttkgesa gemvikidls kesdaylagh
      901 vidgrtlfpa tgymvlvwkt faklqkvdye klpvvfenvq fqratimpke gevkflinif
      961 mgtgefeice ngsavvkgki svpendekml lnipapvvqn qpdlleltpn diykelrlrg
     1021 ydytgvfqgi ksaqnrgvig klqwdnwiay mdtmlqfsil endsrglyvp vrmqylainp
     1081 tvhleyvksf kedegvpvys ysnigvvksg gieirkmkas liskkqqsqs pkydrntfvp
     1141 yenlqpfaqd peraklhalt illqlvqeni pglrikavev agnrpvdvll astvmdiffy
     1201 eplcvvdmql itssrdqyas lesldvkivs rdenngpvgq dlnlvvaanv lsnkalnvlk
     1261 nlsnslkpgg fllleetnqn daslvkdlsl iyvasqsllg ktylllkkke ereeviviqm
     1321 teknfawldn akaalkkaav skqdvifvgq geellgmtgf mncmrreegg wntryvfimd
     1381 knapkfsltd kfyasqlnkq lvanvlkggh wgsyrhlrld qqsgvsslqv ehayinalyr
     1441 gdlsslrwie splsyyqpes fpntefchvy yaplnfrdvm latgklppda lpgnlanqdc
     1501 ilglefsgrn skgkrimamv parglattvv adpgfmwevp dkwtleqaat ipvayatsyy
     1561 slcvrgglkp gdsvlihagt ggvgqasiai alhagckvft tvgtqekrdf lkkifpqltd
     1621 rnignsrdts feqlvlnetn grgvdivlns laeeklqasv rclaiggrfl eigkfdlsnd
     1681 aslgmsvflk nvsfhgilld aicetdgper kevarlvseg ikngavrplp stvfseqqie
     1741 qsfrfmatgk higkvllklr deekqkvvqp spkvvaaipr iymnpdksyi lvgglggfgl
     1801 eladwmiirg akyivltsrs girtgfqslc vrrwremgvh vtistadvtt lagakqlile
     1861 snkiapvggi fnlaavlrda lfenleerdf visslpkivg tknldavsre ycsaldyfvv
     1921 fssvssgrgn lgqtnyglsn samerimeqr qanglpglai ewgaigdvgl iletmgnndt
     1981 evggtlpqri qscletmdvf lqqphpilss fvladkrkna nssnkvsivq avanilgikk
     2041 vesvnpaisl adlgmdslmg teikqtlerg ydvtlsaqdi rgltfeklmk lntdvgeeit
     2101 stsntvsqei gidflfqada asiiptesvv qlttknskgi piffihaieg iaapfkelas
     2161 elnrpawalq cventplssi edlaafyikk iqtiqkkgpy hivgysfgsc iafemsiqlq
     2221 kagqeavvtf idgspdyvkl hsveigkrav enddedvind sfrkalsffi iqfnnkigmr
     2281 eaygilkaep tdekvfnkmv qlinstqika edlkiasvlf hkklyaaymy kpsttyngdv
     2341 llikaednff psesdygise lckktvtvkt lpgnhrsilv gdsaskiasl lqt
//
LOCUS       XP_034178581            2393 aa            linear   INV 03-SEP-2025
DEFINITION  fatty acid synthase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034178581
VERSION     XP_034178581.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322690.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178581.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2393
                     /product="fatty acid synthase 1"
                     /calculated_mol_wt=262524
     Region          27..>844
                     /region_name="PksD"
                     /note="Acyl transferase domain in polyketide synthase
                     (PKS) enzymes [Secondary metabolites biosynthesis,
                     transport and catabolism]; COG3321"
                     /db_xref="CDD:442550"
     Region          877..1081
                     /region_name="hot_dog"
                     /note="The hotdog fold was initially identified in the E.
                     coli FabA (beta-hydroxydecanoyl-acyl carrier protein
                     (ACP)-dehydratase) structure and subsequently in 4HBT
                     (4-hydroxybenzoyl-CoA thioesterase) from Pseudomonas. A
                     number of other seemingly unrelated...; cl00509"
                     /db_xref="CDD:469797"
     Region          1259..>1422
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Region          1469..1757
                     /region_name="PKS_ER"
                     /note="Enoylreductase; smart00829"
                     /db_xref="CDD:214840"
     Site            order(1476,1557,1578,1580..1583,1602..1603,1607,
                     1626..1627,1650,1672,1697..1699,1746..1747,1749,1751)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176179"
     Region          <1778..2014
                     /region_name="KR_1_FAS_SDR_x"
                     /note="beta-ketoacyl reductase (KR) domain of fatty acid
                     synthase (FAS), subgroup 1, complex (x) SDRs; cd08954"
                     /db_xref="CDD:187657"
     Site            order(1795..1796,1798,1818..1821,1846..1849,1873..1875,
                     1896..1897,1921..1922,1936,1963..1964)
                     /site_type="other"
                     /note="putative NADP binding site [chemical binding]"
                     /db_xref="CDD:187657"
     Site            order(1897,1923,1936,1940)
                     /site_type="active"
                     /db_xref="CDD:187657"
     Region          2028..2080
                     /region_name="PKS_PP"
                     /note="Phosphopantetheine attachment site; smart00823"
                     /db_xref="CDD:214834"
     Region          <2114..2392
                     /region_name="EntF2"
                     /note="Thioesterase domain of type I polyketide synthase
                     or non-ribosomal peptide synthetase [Secondary metabolites
                     biosynthesis, transport and catabolism]; COG3319"
                     /db_xref="CDD:442548"
     CDS             1..2393
                     /gene="FASN1"
                     /coded_by="XM_034322690.2:556..7737"
                     /db_xref="GeneID:117603474"
ORIGIN      
        1 mpaqfesans pitreptmmn gdspystged vvitgfsgrf pesvdvdefr knlfegidmv
       61 tdderrwpsg myglptrtgk lkdlksfdat ffgvhakqan vmdpqlrmll eatyeaivda
      121 ginpndirgs ktgvfigvss sesdeywtgd pelvngyglt gccramfsnr isytfdftgp
      181 syaldtacss slyamhqaia airagecdsa ivggcnlllk ptaslqfhrl smlspdgtcr
      241 afdadgkgyv rseavsvvfl qksknarrvy atvvhsktnt dgnkvqgitf psgdmqnklm
      301 revyaeagvn padvvyveah gtgtkvgdpq einsiaklfc kgrktplllg siksnmghae
      361 pasgvssmak lfiametgvi pgnlhfktpn qdipslsdgs iqvidkntpw kgglvainsf
      421 gfgganahil lrsnpkpkls pvldsklpkl vtvsgrteea vevllekake yeyddefval
      481 lhdiyansvt ghlyrgysvm gnvntqevns cvgekrpiwf afsgmgsqwp gmgrellsie
      541 tfqrslhrca dalrpegidl mhliqhgtte tfanvvhsfv siaaiqvalv dllahlgiqp
      601 dgiighsvge lgcayadgtf tpeqtvlaay wrgrmildsk lepggmaavg lsweetkarc
      661 ppnimaacnn sadsvsisgp vedvlkfaae lkqqnifakv vdssgvafhs kyvapagpkl
      721 rtvlekvipn pkqrtarwis tsipeeawgt plaqlsspay hvnnllspvl fhqglahvpe
      781 naivieiaph cllqavlrrs lpptvtnigl hkrdhannig fflenigkly magaqpkvsk
      841 lyppvsypvg rgtpminslv kwdhstewsi adfttkgesa gemvikidls kesdaylagh
      901 vidgrtlfpa tgymvlvwkt faklqkvdye klpvvfenvq fqratimpke gevkflinif
      961 mgtgefeice ngsavvkgki svpendekml lnipapvvqn qpdlleltpn diykelrlrg
     1021 ydytgvfqgi ksaqnrgvig klqwdnwiay mdtmlqfsil endsrglyvp vrmqylainp
     1081 tvhleyvksf kedegvpvys ysnigvvksg gieirkmkas liskkqqsqs pkydrntfvp
     1141 yenlqpfaqd peraklhalt illqlvqeni pglrikavev agnrpvdvll astvmdiffy
     1201 eplcvvdmql itssrdqyas lesldvkivs rdenngpvgq dlnlvvaanv lsnkalnvlk
     1261 nlsnslkpgg fllleetnqn daslvkdlsl iyvasqsllg ktylllkkke ereeviviqm
     1321 teknfawldn akaalkkaav skqdvifvgq geellgmtgf mncmrreegg wntryvfimd
     1381 knapkfsltd kfyasqlnkq lvanvlkggh wgsyrhlrld qqsgvsslqv ehayinalyr
     1441 gdlsslrwie splsyyqpes fpntefchvy yaplnfrdvm latgklppda lpgnlanqdc
     1501 ilglefsgrn skgkrimamv parglattvv adpgfmwevp dkwtleqaat ipvayatsyy
     1561 slcvrgglkp gdsvlihagt ggvgqasiai alhagckvft tvgtqekrdf lkkifpqltd
     1621 rnignsrdts feqlvlnetn grgvdivlns laeeklqasv rclaiggrfl eigkfdlsnd
     1681 aslgmsvflk nvsfhgilld aicetdgper kevarlvseg ikngavrplp stvfseqqie
     1741 qsfrfmatgk higkvllklr deekqkvvqp spkvvaaipr iymnpdksyi lvgglggfgl
     1801 eladwmiirg akyivltsrs girtgfqslc vrrwremgvh vtistadvtt lagakqlile
     1861 snkiapvggi fnlaavlrda lfenleerdf visslpkivg tknldavsre ycsaldyfvv
     1921 fssvssgrgn lgqtnyglsn samerimeqr qanglpglai ewgaigdvgl iletmgnndt
     1981 evggtlpqri qscletmdvf lqqphpilss fvladkrkna nssnkvsivq avanilgikk
     2041 vesvnpaisl adlgmdslmg teikqtlerg ydvtlsaqdi rgltfeklmk lntdvgeeit
     2101 stsntvsqei gidflfqada asiiptesvv qlttknskgi piffihaieg iaapfkelas
     2161 elnrpawalq cventplssi edlaafyikk iqtiqkkgpy hivgysfgsc iafemsiqlq
     2221 kagqeavvtf idgspdyvkl hsveigkrav enddedvind sfrkalsffi iqfnnkigmr
     2281 eaygilkaep tdekvfnkmv qlinstqika edlkiasvlf hkklyaaymy kpsttyngdv
     2341 llikaednff psesdygise lckktvtvkt lpgnhrsilv gdsaskiasl lqt
//
LOCUS       XP_034194591             831 aa            linear   INV 03-SEP-2025
DEFINITION  protocadherin Fat 4-like Cad96Ca isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194591
VERSION     XP_034194591.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338700.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="protocadherin Fat 4-like Cad96Ca isoform X1"
                     /calculated_mol_wt=90473
     Region          39..134
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(44..45,94,96,130,132..133)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          142..241
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(149..150,205,207,238,240..241)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          250..343
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          369..456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(374..375,423,425,453,455..456)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          476..550
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     CDS             1..831
                     /gene="Cad96Cb"
                     /coded_by="XM_034338700.2:487..2982"
                     /db_xref="GeneID:117610849"
ORIGIN      
        1 mrqnilkilp ilcctvhlyt gqlirdarcf lengataahl fvsedlpvgd tvgvlgvlgd
       61 pgpqgdielr lqehdspvaf safsknltlv rpldkegidg pasvyvnvic erkhtldpgf
      121 vipvnirvtd andnapqfvn apyvlnisev tvvgtrvlqg vravdadqpg pfstvqyavl
      181 pgphsdffvf vnalegtlvl rkpldyetls nfsvdiraqd qgnppksstt tlyvnvidad
      241 dqnpafetdq ykiiiprnik trrilkvdpt pikaadqdvg isapvkytiq ggilpfltln
      301 petaevaitr plyehellsp ativvkatqi dnpdkyalst iivsresdwn veptaggrlp
      361 vkfikrdhqt nipentppgs vllttavnkv dsnlrfwlvg aiedlerfsi tnsgelilkg
      421 tldyerriqh sflvrvtdgh hndtsrvnvs vedvnewepr frhpryefha atlregsivg
      481 kleaadgdrd dkvslslrgp darsfeirdn gelilrtaat ingslarlva vasdsgrppr
      541 ssmipvivhi pnsgslpiaa raapawlgss vllvavfgav lgllgviili lilyiykhkr
      601 pktsgsvssv gtgyrekspv psalsptpqv lganmlqdal eiprrgnrne svhsidehde
      661 dadggddped enrvdqeivd dsnhgdvqgl ndsndvenpv fgarnynati ksltsarqmp
      721 lytrhkvapa ptppglsats nipnigglvq nmndlsaktn antasresss ysvdlpdslv
      781 pawpacsvsq rvkklswddd drtvdsveva aetkcrnsqi gnerlnltvy f
//
LOCUS       XP_034194648             807 aa            linear   INV 03-SEP-2025
DEFINITION  protocadherin Fat 4-like Cad96Ca isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194648
VERSION     XP_034194648.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338757.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..807
                     /product="protocadherin Fat 4-like Cad96Ca isoform X2"
                     /calculated_mol_wt=87833
     Region          39..134
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(44..45,94,96,130,132..133)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          142..241
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(149..150,205,207,238,240..241)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          250..343
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          369..456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(374..375,423,425,453,455..456)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          476..550
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     CDS             1..807
                     /gene="Cad96Cb"
                     /coded_by="XM_034338757.2:487..2910"
                     /db_xref="GeneID:117610849"
ORIGIN      
        1 mrqnilkilp ilcctvhlyt gqlirdarcf lengataahl fvsedlpvgd tvgvlgvlgd
       61 pgpqgdielr lqehdspvaf safsknltlv rpldkegidg pasvyvnvic erkhtldpgf
      121 vipvnirvtd andnapqfvn apyvlnisev tvvgtrvlqg vravdadqpg pfstvqyavl
      181 pgphsdffvf vnalegtlvl rkpldyetls nfsvdiraqd qgnppksstt tlyvnvidad
      241 dqnpafetdq ykiiiprnik trrilkvdpt pikaadqdvg isapvkytiq ggilpfltln
      301 petaevaitr plyehellsp ativvkatqi dnpdkyalst iivsresdwn veptaggrlp
      361 vkfikrdhqt nipentppgs vllttavnkv dsnlrfwlvg aiedlerfsi tnsgelilkg
      421 tldyerriqh sflvrvtdgh hndtsrvnvs vedvnewepr frhpryefha atlregsivg
      481 kleaadgdrd dkvslslrgp darsfeirdn gelilrtaat ingslarlva vasdsgrppr
      541 ssmipvivhi pnsgslpiaa raapawlgss vllvavfgav lgllgviili lilyiykhkr
      601 pktsgsvssv gtgyrekspv psalsptpqv lganmlqdal eiprrgnrne svhsidehde
      661 dadggddped enrvdqeivd dsnhgdvqgl ndsndvenpv fgarnynati ksltsarqmp
      721 lytrhkvapa ptppglsats nipnigglvq nmndlsaktn antasresss ysvdlpdslv
      781 pawpacsvsq rvkklswddd drwtalk
//
LOCUS       XP_034177225             616 aa            linear   INV 03-SEP-2025
DEFINITION  V-type proton ATPase catalytic subunit A [Osmia lignaria lignaria].
ACCESSION   XP_034177225
VERSION     XP_034177225.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321334.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..616
                     /product="V-type proton ATPase catalytic subunit A"
                     /calculated_mol_wt=68190
     Region          18..608
                     /region_name="V-ATPase_V1_A"
                     /note="V-type (H+)-ATPase V1, A subunit; TIGR01042"
                     /db_xref="CDD:273411"
     CDS             1..616
                     /gene="LOC117602813"
                     /coded_by="XM_034321334.2:160..2010"
                     /db_xref="GeneID:117602813"
ORIGIN      
        1 mtsrglskis neeretkfgy vyavsgpvvt aekmagsamy elvrvgyyel vgeiirlegd
       61 matiqvyeet sgvtvgdpvl rtgkplsvel gpgilgsifd giqrplkdin eltgsiyipk
      121 ginipalsrt aswefnpsni kngshitggd lfgivyentl vkhkmilppk skgtvtfiap
      181 pgnytvndiv letefdgdrh kysmlqvwpv rqprpvtekl panhplltgq rvldslfpcv
      241 qggttaipga fgcgktvisq alskysnsdv iiyvgcgerg nemsevlrdf peltveidgv
      301 tesimkrtal vantsnmpva areasiytgi tlseyfrdmg ynvsmmadst srwaealrei
      361 sgrlaempad sgypaylgar lasfyeragr vkclgnpdre gsvsivgavs ppggdfsdpv
      421 tsatlgivqv fwgldkklaq rkhfpsinwl isyskylral ddfydknfqe fvplrtkvke
      481 ilqeeedlse ivqlvgkasl aetdkitlev akllkddflq qnsyspydrf cpfyktvgml
      541 rnmiafydma rhavestaqs dnkitwnvir dsmgnilyql ssmkfkdpvk dgeakiradf
      601 dqlhediqqa frnled
//
LOCUS       XP_034178589             615 aa            linear   INV 03-SEP-2025
DEFINITION  equilibrative nucleoside transporter 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178589
VERSION     XP_034178589.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322698.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178589.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..615
                     /product="equilibrative nucleoside transporter 3 isoform
                     X1"
                     /calculated_mol_wt=66935
     Region          41..508
                     /region_name="Nucleoside_tran"
                     /note="Nucleoside transporter; cl15430"
                     /db_xref="CDD:472807"
     CDS             1..615
                     /gene="Ent3"
                     /coded_by="XM_034322698.2:235..2082"
                     /db_xref="GeneID:117603492"
ORIGIN      
        1 mdenlsrgyv qlgkargmne fkfsngfthl sppvdksnfi yfaltlggig fllpynsfii
       61 avdyfqaryp gttvifdmsg vyiimaffav fannilvetl slntritfgy lvsfvtltfv
      121 iicevwwepf gvstsytinl vavavtslgc tvqqssfygy tsmlpsqytq avmtgeslas
      181 lwvsvnrllt ksllddersn tcvyfvlsni tilmcfvlhq ivrktdfvqf yitlcqernr
      241 itleptedvg lmdpldqvgd pskgqygvlk iqtsplgada angstdlngt nqysafsfsn
      301 pvyepgapsg nppgstgpty kvedvvvmrg sygtqtnkpw teikrgllar levakiifpy
      361 masigiayfv tlclypgims eiiscklksw mpvilmtafn asdvlgkmla lipyewkrtq
      421 llyfasarai liplfllcal prgaailsge gypllfacll gvtngivgsv pmmqaptkvp
      481 eshrelagni mtlsyttglt vgsllaymmd tcfglssvha kdvcykvlkl svmpdngtts
      541 silvntvsst lpmletvtpk inttvsvlst ilnstifsns tistlttsmt dasttvsski
      601 fgnvtasvns ailqh
//
LOCUS       XP_076546111             538 aa            linear   INV 03-SEP-2025
DEFINITION  equilibrative nucleoside transporter 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546111
VERSION     XP_076546111.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689996.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..538
                     /product="equilibrative nucleoside transporter 3 isoform
                     X2"
                     /calculated_mol_wt=58273
     Region          1..431
                     /region_name="Nucleoside_tran"
                     /note="Nucleoside transporter; cl15430"
                     /db_xref="CDD:472807"
     CDS             1..538
                     /gene="Ent3"
                     /coded_by="XM_076689996.1:193..1809"
                     /db_xref="GeneID:117603492"
ORIGIN      
        1 msgvyiimaf favfannilv etlslntrit fgylvsfvtl tfviicevww epfgvstsyt
       61 inlvavavts lgctvqqssf ygytsmlpsq ytqavmtges laslwvsvnr lltkslldde
      121 rsntcvyfvl snitilmcfv lhqivrktdf vqfyitlcqe rnritlepte dvglmdpldq
      181 vgdpskgqyg vlkiqtsplg adaangstdl ngtnqysafs fsnpvyepga psgnppgstg
      241 ptykvedvvv mrgsygtqtn kpwteikrgl larlevakii fpymasigia yfvtlclypg
      301 imseiisckl kswmpvilmt afnasdvlgk mlalipyewk rtqllyfasa railiplfll
      361 calprgaail sgegypllfa cllgvtngiv gsvpmmqapt kvpeshrela gnimtlsytt
      421 gltvgsllay mmdtcfglss vhakdvcykv lklsvmpdng ttssilvntv sstlpmletv
      481 tpkinttvsv lstilnstif snstistltt smtdasttvs skifgnvtas vnsailqh
//
LOCUS       XP_076546112             586 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546112
VERSION     XP_076546112.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689997.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="serine/threonine-protein kinase ICK isoform X1"
                     /calculated_mol_wt=67810
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..586
                     /gene="LOC117603499"
                     /coded_by="XM_076689997.1:392..2152"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlessiiyfn ifraqekmvq ptqeqptlpi
      361 lnhnnylrdn npskmdeddf aellgskivp enailaperv ykenranwne nylpdnpnqs
      421 ngnwvlppwn epaaiswnqs qsslkngrkv sgkqhyfsva ryvagqstsl ssrngdaepn
      481 rprlmrnlvg qpmekyeeft dsfwvprnsv enqtkqyylp rnryitdqtl rlpypsvygt
      541 qtiklinlie ttnkgthqpn vygsvlntkg sgglhgrtdw aakylk
//
LOCUS       XP_034178620             586 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178620
VERSION     XP_034178620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="serine/threonine-protein kinase ICK isoform X1"
                     /calculated_mol_wt=67810
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..586
                     /gene="LOC117603499"
                     /coded_by="XM_034322729.2:386..2146"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlessiiyfn ifraqekmvq ptqeqptlpi
      361 lnhnnylrdn npskmdeddf aellgskivp enailaperv ykenranwne nylpdnpnqs
      421 ngnwvlppwn epaaiswnqs qsslkngrkv sgkqhyfsva ryvagqstsl ssrngdaepn
      481 rprlmrnlvg qpmekyeeft dsfwvprnsv enqtkqyylp rnryitdqtl rlpypsvygt
      541 qtiklinlie ttnkgthqpn vygsvlntkg sgglhgrtdw aakylk
//
LOCUS       XP_034178610             586 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178610
VERSION     XP_034178610.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322719.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="serine/threonine-protein kinase ICK isoform X1"
                     /calculated_mol_wt=67810
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..586
                     /gene="LOC117603499"
                     /coded_by="XM_034322719.2:390..2150"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlessiiyfn ifraqekmvq ptqeqptlpi
      361 lnhnnylrdn npskmdeddf aellgskivp enailaperv ykenranwne nylpdnpnqs
      421 ngnwvlppwn epaaiswnqs qsslkngrkv sgkqhyfsva ryvagqstsl ssrngdaepn
      481 rprlmrnlvg qpmekyeeft dsfwvprnsv enqtkqyylp rnryitdqtl rlpypsvygt
      541 qtiklinlie ttnkgthqpn vygsvlntkg sgglhgrtdw aakylk
//
LOCUS       XP_034178630             586 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178630
VERSION     XP_034178630.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322739.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="serine/threonine-protein kinase ICK isoform X1"
                     /calculated_mol_wt=67810
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..586
                     /gene="LOC117603499"
                     /coded_by="XM_034322739.2:369..2129"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlessiiyfn ifraqekmvq ptqeqptlpi
      361 lnhnnylrdn npskmdeddf aellgskivp enailaperv ykenranwne nylpdnpnqs
      421 ngnwvlppwn epaaiswnqs qsslkngrkv sgkqhyfsva ryvagqstsl ssrngdaepn
      481 rprlmrnlvg qpmekyeeft dsfwvprnsv enqtkqyylp rnryitdqtl rlpypsvygt
      541 qtiklinlie ttnkgthqpn vygsvlntkg sgglhgrtdw aakylk
//
LOCUS       XP_034178640             585 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178640
VERSION     XP_034178640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322749.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="serine/threonine-protein kinase ICK isoform X2"
                     /calculated_mol_wt=67723
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..585
                     /gene="LOC117603499"
                     /coded_by="XM_034322749.2:237..1994"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlessiiyfn ifraqekmvq ptqeqptlpi
      361 lnhnnylrdn npskmdeddf aellgkivpe nailapervy kenranwnen ylpdnpnqsn
      421 gnwvlppwne paaiswnqsq sslkngrkvs gkqhyfsvar yvagqstsls srngdaepnr
      481 prlmrnlvgq pmekyeeftd sfwvprnsve nqtkqyylpr nryitdqtlr lpypsvygtq
      541 tiklinliet tnkgthqpnv ygsvlntkgs gglhgrtdwa akylk
//
LOCUS       XP_034178646             580 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178646
VERSION     XP_034178646.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322755.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="serine/threonine-protein kinase ICK isoform X3"
                     /calculated_mol_wt=67114
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..580
                     /gene="LOC117603499"
                     /coded_by="XM_034322755.2:235..1977"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlessiiyfn ifraqekmvq ptqeqptlpi
      361 lnhnnylrdn npskmdeddf aellgskivp enailaperv ykenranwne nylpdnpnqs
      421 ngnwvlppwn epaaiswnqs qsslkngrkv sgkqhyfsva ryvagqstsl ssrngdaepn
      481 rprlmrnlvg qpmekyeeft dsfwvprnsv enqtkqyylp rnryitdqtl rlpypsvygt
      541 qtikttnkgt hqpnvygsvl ntkgsgglhg rtdwaakylk
//
LOCUS       XP_034178654             578 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178654
VERSION     XP_034178654.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322763.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="serine/threonine-protein kinase ICK isoform X4"
                     /calculated_mol_wt=66812
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..578
                     /gene="LOC117603499"
                     /coded_by="XM_034322763.2:235..1971"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlesraqekm vqptqeqptl pilnhnnylr
      361 dnnpskmded dfaellgski vpenailape rvykenranw nenylpdnpn qsngnwvlpp
      421 wnepaaiswn qsqsslkngr kvsgkqhyfs varyvagqst slssrngdae pnrprlmrnl
      481 vgqpmekyee ftdsfwvprn svenqtkqyy lprnryitdq tlrlpypsvy gtqtiklinl
      541 iettnkgthq pnvygsvlnt kgsgglhgrt dwaakylk
//
LOCUS       XP_076546113             577 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546113
VERSION     XP_076546113.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689998.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="serine/threonine-protein kinase ICK isoform X5"
                     /calculated_mol_wt=66724
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..577
                     /gene="LOC117603499"
                     /coded_by="XM_076689998.1:236..1969"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlesraqekm vqptqeqptl pilnhnnylr
      361 dnnpskmded dfaellgkiv penailaper vykenranwn enylpdnpnq sngnwvlppw
      421 nepaaiswnq sqsslkngrk vsgkqhyfsv aryvagqsts lssrngdaep nrprlmrnlv
      481 gqpmekyeef tdsfwvprns venqtkqyyl prnryitdqt lrlpypsvyg tqtiklinli
      541 ettnkgthqp nvygsvlntk gsgglhgrtd waakylk
//
LOCUS       XP_034178664             574 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178664
VERSION     XP_034178664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..574
                     /product="serine/threonine-protein kinase ICK isoform X6"
                     /calculated_mol_wt=66572
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..574
                     /gene="LOC117603499"
                     /coded_by="XM_034322773.2:237..1961"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlessiiyfn ifraqekmvq ptqeqptlpi
      361 lnhnnylrdn npskmdeddf aellgskivp enailaperv ykenranwne nylpdnpnqs
      421 ngnwvlppwn epaaiswnqs qsslkngrkv sgkqhyfsva rngdaepnrp rlmrnlvgqp
      481 mekyeeftds fwvprnsven qtkqyylprn ryitdqtlrl pypsvygtqt iklinliett
      541 nkgthqpnvy gsvlntkgsg glhgrtdwaa kylk
//
LOCUS       XP_034178673             572 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase ICK isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178673
VERSION     XP_034178673.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322782.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="serine/threonine-protein kinase ICK isoform X7"
                     /calculated_mol_wt=66116
     Region          6..286
                     /region_name="STKc_MAK_like"
                     /note="Catalytic domain of Male germ cell-Associated
                     Kinase-like Serine/Threonine Kinases; cd07830"
                     /db_xref="CDD:270824"
     Site            order(12..15,20,33,35,51,65,81..84,87,89..90,127,129,
                     131..132,134,145,148,159,161..164,166,204)
                     /site_type="active"
                     /db_xref="CDD:270824"
     Site            order(12..16,20,33,35,65,81..84,87,127,129,131..132,134,
                     145)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270824"
     Site            order(51,89,127,129,148,159,161..164,166,204)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270824"
     Site            order(144..154,157..166)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270824"
     CDS             1..572
                     /gene="LOC117603499"
                     /coded_by="XM_034322782.2:234..1952"
                     /db_xref="GeneID:117603499"
ORIGIN      
        1 mkmnryitln qlgdgtfgsv vlgeridtge kvaikrmkrk yysweeamnl revkslkkls
       61 hanvvklkev irendvlyfv feymkenlyq lmkdrdklfp epvirnivyq vlqglafmhk
      121 hgffhrdmkp enllcmgpel vkiadfglar eirsrppytd yvstrwyrap evllhsttyn
      181 spidiwavgc imaelytfrp lfpgkseide ifkicsvigt perddwpegy qlaaamnfkf
      241 pnftrtslnv lipnasqeav ilmedmlqwn pikrptaqqs lrypyfqvnv prainskkig
      301 vtsqrdlvln kvnlpppmpk qyysqqdiln tlesraqekm vqptqeqptl pilnhnnylr
      361 dnnpskmded dfaellgski vpenailape rvykenranw nenylpdnpn qsngnwvlpp
      421 wnepaaiswn qsqsslkngr kvsgkqhyfs varyvagqst slssrngdae pnrprlmrnl
      481 vgqpmekyee ftdsfwvprn svenqtkqyy lprnryitdq tlrlpypsvy gtqtikttnk
      541 gthqpnvygs vlntkgsggl hgrtdwaaky lk
//
LOCUS       XP_034178686             444 aa            linear   INV 03-SEP-2025
DEFINITION  COP9 signalosome subunit 3 [Osmia lignaria lignaria].
ACCESSION   XP_034178686
VERSION     XP_034178686.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322795.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..444
                     /product="COP9 signalosome subunit 3"
                     /calculated_mol_wt=50633
     Region          263..367
                     /region_name="PCI"
                     /note="PCI domain; pfam01399"
                     /db_xref="CDD:460195"
     CDS             1..444
                     /gene="CSN3"
                     /coded_by="XM_034322795.2:141..1475"
                     /db_xref="GeneID:117603532"
ORIGIN      
        1 masaleqfvn nvrtlskqgn freltdiitk stdvliknap hldnvletld lqehslgila
       61 vlcvkfslpn pnannvdayk plfnqvqefi igcngeqvrf apdiyaelch ffsqtlvdlk
      121 iplrgiellr rairkiqlfd sqltsihadl cqlcllskcf kpalefldid vtdisqeegq
      181 fdpkyfllyy yyggmiytal knydralyff evcvttpama vsyimleayk kyilvslilh
      241 gkvlnlpryt sqvvnrymkp lsqqyqglat ayqmnsceev qniitkyqql ftrdhnmglv
      301 kqvlsylykk niqrltktfl tlslsdvasr vqlsgpadae kyilnmiedg eifatinqkd
      361 gmvifhddpe kynspqmlak lekemaacmd ldkrvlemee evvltpqyir kacgqndqdd
      421 qtagpaptnv tnvqgqpkhn tysm
//
LOCUS       XP_034194866             358 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611061 [Osmia lignaria lignaria].
ACCESSION   XP_034194866
VERSION     XP_034194866.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338975.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="uncharacterized protein LOC117611061"
                     /calculated_mol_wt=40434
     Region          97..303
                     /region_name="nicotinamidase"
                     /note="Nicotinamidase/pyrazinamidase (PZase).
                     Nicotinamidase, a ubiquitous enzyme in prokaryotes,
                     converts nicotinamide to nicotinic acid (niacin) and
                     ammonia, which in turn can be recycled to make
                     nicotinamide adenine dinucleotide (NAD). The same enzyme
                     is...; cd01011"
                     /db_xref="CDD:238493"
     Site            order(103,223,268)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238493"
     Site            order(148,150,198)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:238493"
     Site            263..264
                     /site_type="active"
                     /note="conserved cis-peptide bond [active]"
                     /db_xref="CDD:238493"
     CDS             1..358
                     /gene="LOC117611061"
                     /coded_by="XM_034338975.2:166..1242"
                     /db_xref="GeneID:117611061"
ORIGIN      
        1 maargtesvt nsfcgkkrrs mekfltefdl lkeetglnyt nfhsicaalf qphelekeer
       61 rledifnlfd gdndgilkgq ewkifihwlr vifepitall vvdvqndfid gslalrrcgc
      121 nqdgvdviep inrlirkghf dniiysldwh penhisfhen lhlrelhlds kvtkenakpl
      181 stvifaepym eqilwpkhcv mntwgaqlhk dlvivphseq vrkgqhpele aysvffdnns
      241 knsvelqtil rksgvthvfv cglaydvcvk atcldglrlg yalaviddcc rgvdtnniet
      301 tkeliiengg litdsntvls lvnegkrsli mshqsakamt silssgtsan dsetidyi
//
LOCUS       XP_034178700             328 aa            linear   INV 03-SEP-2025
DEFINITION  2-oxoglutarate and iron-dependent oxygenase domain-containing
            protein 3 [Osmia lignaria lignaria].
ACCESSION   XP_034178700
VERSION     XP_034178700.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322809.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein 3"
                     /calculated_mol_wt=36994
     Region          <226..310
                     /region_name="P4Hc"
                     /note="Prolyl 4-hydroxylase alpha subunit homologues;
                     smart00702"
                     /db_xref="CDD:214780"
     CDS             1..328
                     /gene="LOC117603537"
                     /coded_by="XM_034322809.2:28..1014"
                     /db_xref="GeneID:117603537"
ORIGIN      
        1 msqetkkgkk kqvtrektnh keekseenak kdsnenvklk ygpsvtfpyq riwsrsalil
       61 tvlfivwyns kqgkeislak qkeillsrtq hvecsndyka etseypecvp ekcgrivsdk
      121 lvsstetdvl lkiamnglsl ggsdggasil dlhsgalskg rdfiniytlp eaktifnsad
      181 faiykvvrtk iqhavahnfg vpankifltk ptffsrmtnv saqtihdeyw hphidketyq
      241 sfdytsllyl ndygrdfegg rfifidkdsv nttveprkgr vsmftsgsen lhavekvqsg
      301 iryaltvsft cnpnaaisdp kyramqek
//
LOCUS       XP_034178709             323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603545 [Osmia lignaria lignaria].
ACCESSION   XP_034178709
VERSION     XP_034178709.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322818.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178709.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..323
                     /product="uncharacterized protein LOC117603545"
                     /calculated_mol_wt=37554
     Region          122..>275
                     /region_name="Cupin_8"
                     /note="Cupin-like domain; pfam13621"
                     /db_xref="CDD:463936"
     CDS             1..323
                     /gene="LOC117603545"
                     /coded_by="XM_034322818.2:1..972"
                     /db_xref="GeneID:117603545"
ORIGIN      
        1 meegdnllen iqeaflslnk syleqgasla dlksigiknd akhkrsfyfh yvcskkvlla
       61 iftpifcsvf fkylyidvmk siretrclip nnyfiweftr pisncdycrg itsalilpnl
      121 treefkryay ssrpmvikna ashwpaskvf swkffkdlye nidgaydsvd ecqflhfksn
      181 ltnlhdvfam seeralqlng kdpwyvgwkn cnlqvldamk kyyslphflp edvevpysny
      241 vflgyeegai mhldyisrlm wqgqiignks wtvaptpecd nvctrfnftv yagdivlldt
      301 riwyhgtyvk dgsfsftvts eyg
//
LOCUS       XP_034178722             297 aa            linear   INV 03-SEP-2025
DEFINITION  WW domain binding protein 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034178722
VERSION     XP_034178722.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..297
                     /product="WW domain binding protein 2 isoform X1"
                     /calculated_mol_wt=32486
     Region          27..129
                     /region_name="PH-GRAM_WBP2"
                     /note="WW binding protein 2 (WB2) Pleckstrin
                     Homology-Glucosyltransferases, Rab-like GTPase activators
                     and Myotubularins (PH-GRAM) domain; cd13214"
                     /db_xref="CDD:275401"
     CDS             1..297
                     /gene="Wbp2"
                     /coded_by="XM_034322831.2:87..980"
                     /db_xref="GeneID:117603553"
ORIGIN      
        1 mslntahtng gvlihageci llfcdnvtme fhgqdqpefi gtkrgrlylt thrmifnakd
       61 qkekmqsfsf pfvtlsevev dqpmfganyi kgkcraqpng nwvgeckfkl hfksggaief
      121 gqamlraasm aqrngpgsda pppyqpptsn wyaapppayq aaptgyygwv pptnvfpevp
      181 pantvfmtdm pppypginap yqgyassapp qgawgsstqq sgwtppqqng apgwanpnyn
      241 aqpmsqagay pnygqppqyn nyssnpppya aypqggnyaq sgyypqqnpy npyprny
//
LOCUS       XP_034178731             291 aa            linear   INV 03-SEP-2025
DEFINITION  WW domain binding protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034178731
VERSION     XP_034178731.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322840.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="WW domain binding protein 2 isoform X2"
                     /calculated_mol_wt=31640
     Region          27..129
                     /region_name="PH-GRAM_WBP2"
                     /note="WW binding protein 2 (WB2) Pleckstrin
                     Homology-Glucosyltransferases, Rab-like GTPase activators
                     and Myotubularins (PH-GRAM) domain; cd13214"
                     /db_xref="CDD:275401"
     CDS             1..291
                     /gene="Wbp2"
                     /coded_by="XM_034322840.2:87..962"
                     /db_xref="GeneID:117603553"
ORIGIN      
        1 mslntahtng gvlihageci llfcdnvtme fhgqdqpefi gtkrgrlylt thrmifnakd
       61 qkekmqsfsf pfvtlsevev dqpmfganyi kgkcraqpng nwvgeckfkl hfksggaief
      121 gqamlraasm aqrngpgsda pppyqpptsn wyaapppayq aaptgyygwv pptnvfpevp
      181 pantvfmtdm pppypginap yqgyassapp qgawgsstqq sgwtppqqng apgwanpnyn
      241 aqpmsqadak aaeaaqsayy dpsrpqcayv pppayyespp sfqqatekkd q
//
LOCUS       XP_034177343             295 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin domain-containing protein UBFD1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177343
VERSION     XP_034177343.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321452.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177343.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="ubiquitin domain-containing protein UBFD1
                     isoform X1"
                     /calculated_mol_wt=32739
     Region          72..141
                     /region_name="Ubl_UBFD1"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     domain-containing protein UBFD1 and similar proteins;
                     cd17047"
                     /db_xref="CDD:340567"
     CDS             1..295
                     /gene="LOC117602879"
                     /coded_by="XM_034321452.2:16..903"
                     /db_xref="GeneID:117602879"
ORIGIN      
        1 mlynimndat kkesndnsfn stscselvsn tnsnedstig tkdsalklev qnsqtqdndn
       61 sitsenssqe nidfkviynk qrinvnfald gtvaelkahl qniisvpqam qkvmikglak
      121 deqtlrslgv tkgakvmvvg sklddvlavs iptkqdlsde aasttnkepl sqqkmhrkvl
      181 dkgipedvmp gilnskeplp efplagmlnk sggkvrltfk leqdqlwigt kertdkipmn
      241 sikgvhsepi hdhpeyhima iqlgpteasr ywiywvpaqy isaikdailg kwcyf
//
LOCUS       XP_034177465             292 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin domain-containing protein UBFD1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177465
VERSION     XP_034177465.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177465.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="ubiquitin domain-containing protein UBFD1
                     isoform X2"
                     /calculated_mol_wt=32382
     Region          69..138
                     /region_name="Ubl_UBFD1"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     domain-containing protein UBFD1 and similar proteins;
                     cd17047"
                     /db_xref="CDD:340567"
     CDS             1..292
                     /gene="LOC117602879"
                     /coded_by="XM_034321574.2:460..1338"
                     /db_xref="GeneID:117602879"
ORIGIN      
        1 meyvdatkke sndnsfnsts cselvsntns nedstigtkd salklevqns qtqdndnsit
       61 senssqenid fkviynkqri nvnfaldgtv aelkahlqni isvpqamqkv mikglakdeq
      121 tlrslgvtkg akvmvvgskl ddvlavsipt kqdlsdeaas ttnkeplsqq kmhrkvldkg
      181 ipedvmpgil nskeplpefp lagmlnksgg kvrltfkleq dqlwigtker tdkipmnsik
      241 gvhsepihdh peyhimaiql gpteasrywi ywvpaqyisa ikdailgkwc yf
//
LOCUS       XP_034177550             292 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin domain-containing protein UBFD1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177550
VERSION     XP_034177550.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321659.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177550.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="ubiquitin domain-containing protein UBFD1
                     isoform X2"
                     /calculated_mol_wt=32382
     Region          69..138
                     /region_name="Ubl_UBFD1"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin
                     domain-containing protein UBFD1 and similar proteins;
                     cd17047"
                     /db_xref="CDD:340567"
     CDS             1..292
                     /gene="LOC117602879"
                     /coded_by="XM_034321659.2:130..1008"
                     /db_xref="GeneID:117602879"
ORIGIN      
        1 meyvdatkke sndnsfnsts cselvsntns nedstigtkd salklevqns qtqdndnsit
       61 senssqenid fkviynkqri nvnfaldgtv aelkahlqni isvpqamqkv mikglakdeq
      121 tlrslgvtkg akvmvvgskl ddvlavsipt kqdlsdeaas ttnkeplsqq kmhrkvldkg
      181 ipedvmpgil nskeplpefp lagmlnksgg kvrltfkleq dqlwigtker tdkipmnsik
      241 gvhsepihdh peyhimaiql gpteasrywi ywvpaqyisa ikdailgkwc yf
//
LOCUS       XP_034194940             288 aa            linear   INV 03-SEP-2025
DEFINITION  CD209 antigen-like protein A [Osmia lignaria lignaria].
ACCESSION   XP_034194940
VERSION     XP_034194940.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339049.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="CD209 antigen-like protein A"
                     /calculated_mol_wt=32358
     Region          135..259
                     /region_name="CLECT"
                     /note="C-type lectin (CTL) or carbohydrate-recognition
                     domain (CRD); smart00034"
                     /db_xref="CDD:214480"
     Site            order(229,233,235,241,243..245,249,252..254)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..288
                     /gene="LOC117611113"
                     /coded_by="XM_034339049.2:158..1024"
                     /db_xref="GeneID:117611113"
ORIGIN      
        1 mkrkgslhlh slllifqlsv cmgipgnlhp vsvspnasit etsigpwgei lenwivsdsk
       61 isrtskvmkf gdktltvstk vstpnkrevs etdlyllgai eklvyrvdfl enrlrraeel
      121 lyyvisgnie kkescpqnyt kigqncyhfs srdfdwkssa slcrgmgghl iefdtddekr
      181 dvfaslqtns klkgktfwtg glnpgllwiw assakpvhqn tkqtvpgdgr clklsynsts
      241 rlysyqsddc garhryackl tvddesvnki eksvrmlmng krlstntd
//
LOCUS       XP_034177671             260 aa            linear   INV 03-SEP-2025
DEFINITION  upstream stimulatory factor 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034177671
VERSION     XP_034177671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="upstream stimulatory factor 1 isoform X1"
                     /calculated_mol_wt=28294
     Region          139..207
                     /region_name="bHLHzip_USF"
                     /note="basic Helix-Loop-Helix-zipper (bHLHzip) domain
                     found in upstream stimulatory factors, USF1, USF2 and
                     similar proteins; cd11396"
                     /db_xref="CDD:381402"
     Site            order(139,141,144..148,152,186..189)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381402"
     Site            order(151,154,157,161,192,196,199..200,202..203,207)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381402"
     Region          <217..245
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     Site            order(217,219..224,226..227,229..230,233..234,236..237,
                     240..241,243..244)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409275"
     CDS             1..260
                     /gene="LOC117603038"
                     /coded_by="XM_034321780.2:107..889"
                     /db_xref="GeneID:117603038"
ORIGIN      
        1 mdileqpleq qdvstvnakd dggtgiviee aeivdceget vgedgmryqe tlayrvvqvn
       61 gttagneiel pvtqpgnntv qvltsplngq fyvignandv fttaqtsrsl vprtttlqie
      121 tprncttvlk krddrrrath neverrrrdk innwiaklgk iipecntsan gngsgsgegk
      181 anyetqskgg ilakaceyig elraanqglg qclrdneklr qeitalkqlv aqlkrenlql
      241 rsqissssag tnvdvvhlsp
//
LOCUS       XP_034177754             215 aa            linear   INV 03-SEP-2025
DEFINITION  upstream stimulatory factor 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034177754
VERSION     XP_034177754.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321863.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="upstream stimulatory factor 1 isoform X2"
                     /calculated_mol_wt=23563
     Region          94..162
                     /region_name="bHLHzip_USF"
                     /note="basic Helix-Loop-Helix-zipper (bHLHzip) domain
                     found in upstream stimulatory factors, USF1, USF2 and
                     similar proteins; cd11396"
                     /db_xref="CDD:381402"
     Site            order(94,96,99..103,107,141..144)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381402"
     Site            order(106,109,112,116,147,151,154..155,157..158,162)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381402"
     Region          <170..200
                     /region_name="ZIP_TSC22D-like"
                     /note="leucine zipper found in the TSC22 domain leucine
                     zipper transcription factors, c-Myc-binding protein, and
                     similar proteins; cl40461"
                     /db_xref="CDD:424092"
     Site            order(171..172,174..179,181..182,184..185,188..189,
                     191..192,195..196,198..199)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409275"
     CDS             1..215
                     /gene="LOC117603038"
                     /coded_by="XM_034321863.2:370..1017"
                     /db_xref="GeneID:117603038"
ORIGIN      
        1 mryqetlayr vvqvngttag neielpvtqp gnntvqvlts plngqfyvig nandvfttaq
       61 tsrslvprtt tlqietprnc ttvlkkrddr rrathnever rrrdkinnwi aklgkiipec
      121 ntsangngsg sgegkanyet qskggilaka ceyigelraa nqglgqclrd neklrqeita
      181 lkqlvaqlkr enlqlrsqis sssagtnvdv vhlsp
//
LOCUS       XP_076546114             196 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinamide riboside kinase [Osmia lignaria lignaria].
ACCESSION   XP_076546114
VERSION     XP_076546114.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076689999.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="nicotinamide riboside kinase"
                     /calculated_mol_wt=23234
     Region          8..165
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     Site            order(13,18..21)
                     /site_type="active"
                     /db_xref="CDD:238977"
     CDS             1..196
                     /gene="Nrk1"
                     /coded_by="XM_076689999.1:1..591"
                     /db_xref="GeneID:117603557"
ORIGIN      
        1 msskkwlvig itgatcsgkt tlanrlqkel knsiivhqdn yfwpiddpqh vkipklnhln
       61 welmssmdmd kmrsdilkli efspndvnkm eknckrvlii dgfllfkckv itnlcdikyf
      121 ftvskeisre rrekreyepr dvpgyfeeyv wpeylkykde imkdknlckt itfldgskdt
      181 eeifqmifpr ikqmls
//
LOCUS       XP_034195025             192 aa            linear   INV 03-SEP-2025
DEFINITION  5'-deoxynucleotidase HDDC2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195025
VERSION     XP_034195025.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339134.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..192
                     /product="5'-deoxynucleotidase HDDC2 isoform X1"
                     /calculated_mol_wt=22363
     Region          21..173
                     /region_name="HD_3"
                     /note="HD domain; pfam13023"
                     /db_xref="CDD:432939"
     CDS             1..192
                     /gene="LOC117611174"
                     /coded_by="XM_034339134.2:16..594"
                     /db_xref="GeneID:117611174"
ORIGIN      
        1 mfvekvdivf ifcylrilhm krtgwvirni pepetiaghm yrmamlsflv dnkenldkvk
       61 imqmalihdl aecivgditp hcgvpleekh rredeamedi ckllgdkgpm ilemfreyek
      121 qespearyvk dldrldlimq ayeyekrdnv pgeleeffva tngkirhpfi nkiaceitar
      181 realchnsts sk
//
LOCUS       XP_034195063             191 aa            linear   INV 03-SEP-2025
DEFINITION  5'-deoxynucleotidase HDDC2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195063
VERSION     XP_034195063.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..191
                     /product="5'-deoxynucleotidase HDDC2 isoform X2"
                     /calculated_mol_wt=22240
     Region          13..172
                     /region_name="HD_3"
                     /note="HD domain; pfam13023"
                     /db_xref="CDD:432939"
     CDS             1..191
                     /gene="LOC117611174"
                     /coded_by="XM_034339172.2:266..841"
                     /db_xref="GeneID:117611174"
ORIGIN      
        1 memeklqefm ellgrlkhmk rtgwvirnip epetiaghmy rmamlsflvd nkenldkvki
       61 mqmalihdla ecivgditph cgvpleekhr redeamedic kllgdkgpmi lemfreyekq
      121 espearyvkd ldrldlimqa yeyekrdnvp geleeffvat ngkirhpfin kiaceitarr
      181 ealchnstss k
//
LOCUS       XP_034195130             173 aa            linear   INV 03-SEP-2025
DEFINITION  5'-deoxynucleotidase HDDC2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195130
VERSION     XP_034195130.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339239.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..173
                     /product="5'-deoxynucleotidase HDDC2 isoform X3"
                     /calculated_mol_wt=19995
     Region          2..154
                     /region_name="HD_3"
                     /note="HD domain; pfam13023"
                     /db_xref="CDD:432939"
     CDS             1..173
                     /gene="LOC117611174"
                     /coded_by="XM_034339239.2:138..659"
                     /db_xref="GeneID:117611174"
ORIGIN      
        1 mkrtgwvirn ipepetiagh myrmamlsfl vdnkenldkv kimqmalihd laecivgdit
       61 phcgvpleek hrredeamed ickllgdkgp milemfreye kqespearyv kdldrldlim
      121 qayeyekrdn vpgeleeffv atngkirhpf inkiaceita rrealchnst ssk
//
LOCUS       XP_034178781             140 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603565 [Osmia lignaria lignaria].
ACCESSION   XP_034178781
VERSION     XP_034178781.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178781.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="uncharacterized protein LOC117603565"
                     /calculated_mol_wt=16054
     Region          21..138
                     /region_name="AROS"
                     /note="Active regulator of SIRT1, or 40S ribosomal protein
                     S19-binding 1; pfam15684"
                     /db_xref="CDD:464802"
     CDS             1..140
                     /gene="LOC117603565"
                     /coded_by="XM_034322890.2:70..492"
                     /db_xref="GeneID:117603565"
ORIGIN      
        1 msnslvrkgl ellgyektvk qekkkrkhtn ykgtldlipa khrivskndk tdlgtilgrs
       61 skvtvyetkk klsaqedptd knvkkllvls nnrmdpntvn klldravkkr yipkphkpkg
      121 pettafteed fkkfekeyie
//
LOCUS       XP_034178795             825 aa            linear   INV 03-SEP-2025
DEFINITION  dynein regulatory complex protein 11 [Osmia lignaria lignaria].
ACCESSION   XP_034178795
VERSION     XP_034178795.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322904.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178795.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..825
                     /product="dynein regulatory complex protein 11"
                     /calculated_mol_wt=95509
     Region          <119..290
                     /region_name="DUF4670"
                     /note="Domain of unknown function (DUF4670); pfam15709"
                     /db_xref="CDD:464815"
     Region          200..231
                     /region_name="IQCD"
                     /note="IQ (isoleucine-glutamine) motif containing D
                     (IQCD); cd23767"
                     /db_xref="CDD:467745"
     Region          531..689
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            order(568..570,586,588..589,624..625)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:410865"
     CDS             1..825
                     /gene="LOC117603570"
                     /coded_by="XM_034322904.2:14..2491"
                     /db_xref="GeneID:117603570"
ORIGIN      
        1 msnttydelw knaqamldel ldmdsslqna kpqkdrkkah nvtselyvry ifvcnklelc
       61 ydqiiqpqkr vlikklldsc lgrvlelkhe lveidlseys yfddilmkln vtpqeielqv
      121 pryfkrenmk eiderrkfie ntlrnigaln eviplkqmte seairliqah erarqsrirf
      181 qfmkeirrmk eksiikteae iqdelskiaa atkiqkvwrg yvtrqkirkr rleemlligm
      241 lqpsqviten arqaertrqe rydkqikyqe lyenlmietk erirkeknai ieenirsevr
      301 nwinsyfqqt gkipdlpsve sggsriilsr qgtestisks taasskesrk skkskskkds
      361 egeqseeete qgfksmpsnf lselvhasqe yqevwknkde smnaaqfpyl dmiesdkake
      421 venevritvd qelrgdieal qnaldrdkgf kgkrikkgqk rirhsgkknk krkekdltpd
      481 rtteslfeel ltqgiiklhr eislgdfkge ksfinhnlre kgkdplptlg dirqliteyc
      541 ilplgnntlr eltplvrsvl iagprysgkk mlvnaictel gatlfditpa niagkypgks
      601 glimlvhlis kvsrllqpsv ifmdgaeklf tkkvaktdkt dpkrlkkdlp klvknitned
      661 rviligtsgs pwdgdqklly qtydkviyip rpdygtmslv wkdllykysg inrqfdtsvi
      721 akvcdgftig avltsinevm ttkrivqlkt hpltygelin avstknpiyc eeedaflawl
      781 aktpiyrkkq rmheqelqkl keanekqkkk gkkstrinll iptek
//
LOCUS       XP_076546115             112 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611928 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546115
VERSION     XP_076546115.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690000.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..112
                     /product="uncharacterized protein LOC117611928 isoform X1"
                     /calculated_mol_wt=13281
     CDS             1..112
                     /gene="LOC117611928"
                     /coded_by="XM_076690000.1:916..1254"
                     /db_xref="GeneID:117611928"
ORIGIN      
        1 mellhkekkr keqrkwkrsa eedgrremeg gnsvkkeegs mandikekrq rirvnleaan
       61 aekptevraf sayewrrelf vtrgkndgrr edkriritvi crqcraiich dl
//
LOCUS       XP_076546116             112 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611928 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546116
VERSION     XP_076546116.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690001.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..112
                     /product="uncharacterized protein LOC117611928 isoform X1"
                     /calculated_mol_wt=13281
     CDS             1..112
                     /gene="LOC117611928"
                     /coded_by="XM_076690001.1:916..1254"
                     /db_xref="GeneID:117611928"
ORIGIN      
        1 mellhkekkr keqrkwkrsa eedgrremeg gnsvkkeegs mandikekrq rirvnleaan
       61 aekptevraf sayewrrelf vtrgkndgrr edkriritvi crqcraiich dl
//
LOCUS       XP_076546117              91 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611928 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546117
VERSION     XP_076546117.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690002.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..91
                     /product="uncharacterized protein LOC117611928 isoform X2"
                     /calculated_mol_wt=10811
     CDS             1..91
                     /gene="LOC117611928"
                     /coded_by="XM_076690002.1:919..1194"
                     /db_xref="GeneID:117611928"
ORIGIN      
        1 mellhkekkr keqrkwkrsa eedgrremeg gnsvkkeegs mandikekrq rirvnleaan
       61 aekptevrdf lfssfqrirv eervvrnare k
//
LOCUS       XP_076546118              84 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611928 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546118
VERSION     XP_076546118.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690003.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..84
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..84
                     /product="uncharacterized protein LOC117611928 isoform X3"
                     /calculated_mol_wt=9741
     CDS             1..84
                     /gene="LOC117611928"
                     /coded_by="XM_076690003.1:919..1173"
                     /db_xref="GeneID:117611928"
ORIGIN      
        1 mellhkekkr keqrkwkrsa eedgrremeg gnsvkkeegs mandikekrq rirvnleaan
       61 aekptevrgn rgflrkthqr avdg
//
LOCUS       XP_034180206             190 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L9 [Osmia lignaria lignaria].
ACCESSION   XP_034180206
VERSION     XP_034180206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324315.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..190
                     /product="ribosomal protein L9"
                     /calculated_mol_wt=21364
     Region          1..189
                     /region_name="PTZ00027"
                     /note="60S ribosomal protein L6; Provisional"
                     /db_xref="CDD:240234"
     CDS             1..190
                     /gene="RpL9"
                     /coded_by="XM_034324315.2:75..647"
                     /db_xref="GeneID:117604351"
ORIGIN      
        1 mkqivtnqtv kipegltvtv ksrlvtvkgp rgvlkrsfkh laldirmvsp rllkvekwfg
       61 tkkelaavrt vcshienmlk gvtkgyqykm ravyahfpin cvttenntvi eirnflgeky
      121 irrvkmapgv tvtnsakqkd eliiegnsle dvsrsaaliq qsttvknkdi rkfldglyvs
      181 ekttvvqdde
//
LOCUS       XP_076546119             231 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305650 [Osmia lignaria lignaria].
ACCESSION   XP_076546119
VERSION     XP_076546119.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690004.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..231
                     /product="uncharacterized protein LOC143305650"
                     /calculated_mol_wt=23868
     CDS             1..231
                     /gene="LOC143305650"
                     /coded_by="XM_076690004.1:27..722"
                     /db_xref="GeneID:143305650"
ORIGIN      
        1 mpttvdpfkg sswtsqrika edllgissva kqrptksdss tmnaklfiaf afvavafaee
       61 qsedtltetk kekrgiygig ygghgghggy gggygggygg gygggggyav pvavpvpqpv
      121 avpvdrhvpy pvkvpvavpv drpypvhvpr pypvevtrhv pvpvdrpvpv pvsvpvkvpv
      181 papyavrvpq pyavpvvkpv avpvgqptvi veksnngwtv gggggggyss w
//
LOCUS       XP_034195427             201 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611568 [Osmia lignaria lignaria].
ACCESSION   XP_034195427
VERSION     XP_034195427.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339536.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="uncharacterized protein LOC117611568"
                     /calculated_mol_wt=21816
     CDS             1..201
                     /gene="LOC117611568"
                     /coded_by="XM_034339536.2:127..732"
                     /db_xref="GeneID:117611568"
ORIGIN      
        1 miriflfvla vaavkashfs leghgygsep klisqsvhlk eiptkiikvt ktavvkvpvp
       61 ypvkvphhip vpvpvnhpia ipytklvkvq ehvpvevskp ypveihqqvp vvvpkavpip
      121 qpvpvpqpvt lnkpvfypvp hpipyqahsq seesvggvgg ydsgdhgghe sesystytvn
      181 qqghdqggnf qpsqpdysdh h
//
LOCUS       XP_034179458             181 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603941 [Osmia lignaria lignaria].
ACCESSION   XP_034179458
VERSION     XP_034179458.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323567.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179458.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..181
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..181
                     /product="uncharacterized protein LOC117603941"
                     /calculated_mol_wt=18319
     CDS             1..181
                     /gene="LOC117603941"
                     /coded_by="XM_034323567.2:267..812"
                     /db_xref="GeneID:117603941"
ORIGIN      
        1 mralvcvmli avafaaeekt vqkrgllglg glgdlgyggh gllggslghg svavaihdrp
       61 vavpvpvpkp ypvavdrpvp vkvpvavphp vpvavpvpqp ypvvqtktit vpvdrpvpvs
      121 vpvkvpvpvp apypvkvavp havpvavpqp vlvketvpil vhghglghgl ghglghglgh
      181 y
//
LOCUS       XP_034172604            1695 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate cyclase type 10 [Osmia lignaria lignaria].
ACCESSION   XP_034172604
VERSION     XP_034172604.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316713.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172604.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1695
                     /product="adenylate cyclase type 10"
                     /calculated_mol_wt=194859
     Region          72..238
                     /region_name="CHD"
                     /note="cyclase homology domain; cd07302"
                     /db_xref="CDD:143636"
     Site            order(75,77..82,123,125..127,130,196..198,205..206)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(77,127)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(82,100,103..104,107,111,123..124,182,185,197..200,
                     206)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          354..523
                     /region_name="CHD"
                     /note="cyclase homology domain; cd07302"
                     /db_xref="CDD:143636"
     Site            order(359,361..362,396,399..401,407,467..469,473..474,
                     477..478,513)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(361,401)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(373,376..377,380,384,396..397,454,457,468..471,474,
                     513)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     Region          <541..>814
                     /region_name="COG3899"
                     /note="Predicted ATPase [General function prediction
                     only]"
                     /db_xref="CDD:443106"
     CDS             1..1695
                     /gene="LOC117600805"
                     /coded_by="XM_034316713.2:214..5301"
                     /db_xref="GeneID:117600805"
ORIGIN      
        1 mlghvnsaar qawrnkrkrm knegikhyve metkskvaql rleyhtkifa smcpdeildh
       61 yddyhtrmyy ttlmlgdvsg ftdlaekytk tgrggpsklt etlnsyigam vqeilshngd
      121 vlkfsgdafi vmwklhegmm mrdlaieamq taciiqkhfg tydtdvgvtl rvklaiasgk
      181 tyftsigdpe srshyiitgq pvwdvkfaeg lcrggdilva psswqwvnpn eyvheklpdg
      241 ihtlivacts mwyhprdeei pigstededt eiyrdthdsl lmsrttidpl sdimlagnky
      301 nsqifkqvdy slrpkvvkia karlkdalrs ymlrpvvrsv emdepleylt emrqvvilfi
      361 nvitsniskr alialvnaty klvgqivgem hgcvnktslf dkdlmflcvf glrgdkhele
      421 sqiglrcask lrsnlveldn vksvtvgvtt gmtycgvvgh ilrreytvig msvnkaarlm
      481 vayrdkvvcd resflhshle arhfilqepr ylkgitnvgp iyefqeqpky tvsdlvwnky
      541 pllgreteik lfrallmkll eysaleknad ttrpeyntli ikgeprigkt rmldeftqni
      601 plgvrcnyis lhtedakapf slvhlifsvp lgftvtstrk eredklllrl gkvkrphylc
      661 ilnqpfnvrf sishrynils epqrqkvlrk fllkllkgcf ddlwiiivdd aeysdneslq
      721 vfdvltkkdk vlfilsvgmk lsteykmcpn ilsrakvinl pgidrwyhag lacqilnvsg
      781 ipaelekliq ersfgnpgwi esylvsllqv ggleiinisk keanmkgyvl ppvtmlkrfm
      841 sklshdvred rndrwqmyrt sfkdsvlsll eqdtngihks aanleseegm iavcnlsenf
      901 tyedvnpeit mdvmilklfd sltpldqlll kcasvigetv nrhmlehlmt itskreigla
      961 vlklfeirvf gcaigdfsrn tgpiifirni rnptseidvf cnciglhipe aledlpryas
     1021 cglmrfkmtm frdttyrllt enqkmelhnq alkylqqytr rcvscgegqf akllgkvskk
     1081 eerrkrttni ddifgtvtfd eivvagderk kekaflacld ifrgmekkpt atfsdadfad
     1141 chchlilmtv ytqilehcrg igniemtlva ilefveicll ysnipqarrl lsegetvlge
     1201 lfkskddemv mlpyltskiq tlqgrcfles gsifeaehil emalenlgyk fprleimidl
     1261 sstvqliqlk mnltctkdwt ldnnyvtdsy dyteqlaecl tqmfdlfrik gmkrharlaa
     1321 iwglnaalts nkdlfilcas ytnmlitahm yqdrtvipyl eqksinicne crdaleaqel
     1381 nlvaelyagi ffsrwvrgqi skatqigfic srmastigst flkllilprl ahllmiscrh
     1441 sevitqlrel efvsrndldk sgltwyyalc advhldtglt vlsfdsceqy flregeqmis
     1501 fhdpeaerry ftsmwlwcvr teqweaakvw sgrsvesapi mdehivaatm tsckklegll
     1561 vlyvhkinsr nvdatitmrq iehmlkelkk tvkivrimip rymllkgyyy miqfrkntal
     1621 kmlkrlkkvc qkmenkmiyt wamhcekaws grispvqedl wkeisqsqmk ikwdeinvnd
     1681 snmvsftfpl pkysn
//
LOCUS       XP_034186958             136 aa            linear   INV 03-SEP-2025
DEFINITION  helix-loop-helix protein 15 [Osmia lignaria lignaria].
ACCESSION   XP_034186958
VERSION     XP_034186958.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331067.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="helix-loop-helix protein 15"
                     /calculated_mol_wt=15362
     Region          64..135
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(87..88,90,95,115..116)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381392"
     Site            order(93..94,97..98,100..101,105,116..117,120..121,124,
                     126..127,130..131)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381392"
     CDS             1..136
                     /gene="LOC117607386"
                     /coded_by="XM_034331067.1:313..723"
                     /db_xref="GeneID:117607386"
ORIGIN      
        1 mkipfhtnra pirnehrkvt eiqgncrrki lflktgvssi eekmangghg vvvngvgapg
       61 agtlsreerr rrrratqkyr tahatrervr veafnlafae lrkllptlpp dkklskieil
      121 rlaicyiayl nhvlea
//
LOCUS       XP_034187043             136 aa            linear   INV 03-SEP-2025
DEFINITION  helix-loop-helix protein 15 [Osmia lignaria lignaria].
ACCESSION   XP_034187043
VERSION     XP_034187043.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331152.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="helix-loop-helix protein 15"
                     /calculated_mol_wt=15362
     Region          64..135
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(87..88,90,95,115..116)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381392"
     Site            order(93..94,97..98,100..101,105,116..117,120..121,124,
                     126..127,130..131)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381392"
     CDS             1..136
                     /gene="LOC117607386"
                     /coded_by="XM_034331152.2:616..1026"
                     /db_xref="GeneID:117607386"
ORIGIN      
        1 mkipfhtnra pirnehrkvt eiqgncrrki lflktgvssi eekmangghg vvvngvgapg
       61 agtlsreerr rrrratqkyr tahatrervr veafnlafae lrkllptlpp dkklskieil
      121 rlaicyiayl nhvlea
//
LOCUS       XP_034171817             170 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600456 [Osmia lignaria lignaria].
ACCESSION   XP_034171817
VERSION     XP_034171817.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315926.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171817.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..170
                     /product="uncharacterized protein LOC117600456"
                     /calculated_mol_wt=18502
     CDS             1..170
                     /gene="LOC117600456"
                     /coded_by="XM_034315926.2:152..664"
                     /db_xref="GeneID:117600456"
ORIGIN      
        1 mrileflllf slnalfyacl akpaemlsnp gslnpvqlme vvsvdadqnr eqrspqfgll
       61 gedysdysee eeeertfdhh rkhkhhkhkp egfhghptgh yggyqppehy yppstggggs
      121 fatasagsid shgkpfggqs hanaqsasfs fgpysatfsv aeaasgsqry
//
LOCUS       XP_076546120             170 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600456 [Osmia lignaria lignaria].
ACCESSION   XP_076546120
VERSION     XP_076546120.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690005.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..170
                     /product="uncharacterized protein LOC117600456"
                     /calculated_mol_wt=18502
     CDS             1..170
                     /gene="LOC117600456"
                     /coded_by="XM_076690005.1:158..670"
                     /db_xref="GeneID:117600456"
ORIGIN      
        1 mrileflllf slnalfyacl akpaemlsnp gslnpvqlme vvsvdadqnr eqrspqfgll
       61 gedysdysee eeeertfdhh rkhkhhkhkp egfhghptgh yggyqppehy yppstggggs
      121 fatasagsid shgkpfggqs hanaqsasfs fgpysatfsv aeaasgsqry
//
LOCUS       XP_034171828             144 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600462 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171828
VERSION     XP_034171828.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315937.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171828.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..144
                     /product="uncharacterized protein LOC117600462 isoform X1"
                     /calculated_mol_wt=15970
     CDS             1..144
                     /gene="LOC117600462"
                     /coded_by="XM_034315937.2:123..557"
                     /db_xref="GeneID:117600462"
ORIGIN      
        1 msskrvhslv tkisallfks ksrrkmtnfl alsvialacv ivngqqtsdq kpvfgqtfde
       61 ivvssdlnvr rkskenrqgk tlfnipsltt gppekstava arftandgsq vtseksetkr
      121 eisnlekryf dmdvaayllk srkr
//
LOCUS       XP_034171837             119 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600462 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171837
VERSION     XP_034171837.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315946.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171837.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..119
                     /product="uncharacterized protein LOC117600462 isoform X2"
                     /calculated_mol_wt=13100
     CDS             1..119
                     /gene="LOC117600462"
                     /coded_by="XM_034315946.2:191..550"
                     /db_xref="GeneID:117600462"
ORIGIN      
        1 mtnflalsvi alacvivngq qtsdqkpvfg qtfdeivvss dlnvrrkske nrqgktlfni
       61 pslttgppek stavaarfta ndgsqvtsek setkreisnl ekryfdmdva ayllksrkr
//
LOCUS       XP_034171852             133 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600472 [Osmia lignaria lignaria].
ACCESSION   XP_034171852
VERSION     XP_034171852.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315961.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..133
                     /product="uncharacterized protein LOC117600472"
                     /calculated_mol_wt=14750
     CDS             1..133
                     /gene="LOC117600472"
                     /coded_by="XM_034315961.1:63..464"
                     /db_xref="GeneID:117600472"
ORIGIN      
        1 mkflllfvfv avvvmidarp skssvpstkk neeikealki snqvegelee lertkkhihr
       61 mqypgwgsss vnvaqasasv hsnpghyhhs hhrlphysss hsethdfaan vnlphsslsv
      121 aqseahqhqq tfg
//
LOCUS       XP_034172635             392 aa            linear   INV 03-SEP-2025
DEFINITION  kin17 DNA and RNA binding protein [Osmia lignaria lignaria].
ACCESSION   XP_034172635
VERSION     XP_034172635.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316744.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172635.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="kin17 DNA and RNA binding protein"
                     /calculated_mol_wt=45457
     Region          55..177
                     /region_name="Kin17_mid"
                     /note="Domain of Kin17 curved DNA-binding protein;
                     pfam10357"
                     /db_xref="CDD:463057"
     Region          272..329
                     /region_name="KN17_SH3"
                     /note="KN17 SH3-like C-terminal domain; pfam18131"
                     /db_xref="CDD:407963"
     Region          340..390
                     /region_name="KOW"
                     /note="an acronym for the authors' surnames (Kyrpides,
                     Ouzounis and Woese); cl00354"
                     /db_xref="CDD:469738"
     CDS             1..392
                     /gene="kin17"
                     /coded_by="XM_034316744.2:284..1462"
                     /db_xref="GeneID:117600819"
ORIGIN      
        1 mgkhevgtpk yiankikakg lqklrwycqm cqkqcrdeng fkchtmsesh hrqlllfadn
       61 asrymdqfsr efshgylnll krqfgtrrvp anrvyqeyis drghvhmnat iwvtltgfvk
      121 wlgrtgqcvv detekgwyvt yidrdpetla aqekkakkqk mdkddeermm efiekqvekg
      181 qqetnvqnea ykepltrpen dtplvlelki nkkpklllss vkqekvtdmg stsseaisvs
      241 nikceqlsen eeesslkktd khkskhdpdd sneegwlren lmvkvitktl geryykakgi
      301 iesiensnfv gkvklkspee venhvikidq eyletvipai gkdiiilwgk ykgmkgvvhk
      361 vhienycidv klkendtvvk klpyeqvcky vt
//
LOCUS       XP_034189296            1094 aa            linear   INV 03-SEP-2025
DEFINITION  O-linked N-acetylglucosamine (GlcNAc) transferase sxc isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034189296
VERSION     XP_034189296.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333405.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1094
                     /product="O-linked N-acetylglucosamine (GlcNAc)
                     transferase sxc isoform X1"
                     /calculated_mol_wt=122315
     Region          73..98
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          100..338
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          106..132
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          138..166
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(139,142..143,146..147,149,173,176..177,180..181,
                     183..184,207,210..211,214..215,218)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          171..201
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          206..234
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          239..506
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          240..268
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(275,278..279,282..283,285,309,312..313,316..317,
                     319..320,343,346..347,350..351,354)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          275..302
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          307..337
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          342..370
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          376..404
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          410..438
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(411,414..415,418..419,421,445,448..449,452..453,
                     455..456,479,482..483,486..487,490)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          443..473
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          478..506
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          525..1059
                     /region_name="Glyco_transf_41"
                     /note="Glycosyl transferase family 41; pfam13844"
                     /db_xref="CDD:404688"
     CDS             1..1094
                     /gene="sxc"
                     /coded_by="XM_034333405.2:593..3877"
                     /db_xref="GeneID:117608373"
ORIGIN      
        1 mslrlaekke nvlknstsii gnvvnssddv cngtvewqng qnllvvtqdt qgkeliaiad
       61 nqtinvavds ywllelahre yqagdyenae rhcmqlwrqe tnntgvllll ssihfqcrrl
      121 eksahyssla ikqnpllaea ysnlgnvfke rgqlqealen yrhavrlkpd fidgyinlaa
      181 alvaagdmeq avqayvtalq ynpdlycvrs dlgnllkala rldeakacyl kaietrpdfa
      241 vawsnlgcvf naqgeiwlai hhfekavald pnfldayinl gnvlkearif dravaaylra
      301 lnlspnnavv hgnlacvyye qglidlaidt yrraielqpn fpdaycnlan alkekgqvve
      361 aedcyntalr lcpshadsln nlanikreqg yieeatrlyl kalevfpefa aahsnlasvl
      421 qqqgklneal mhykeairiq ptfadaysnm gntlkemqdi qgalqcytra iqinpafada
      481 hsnlasihkd sgnipeaiqs yrtalklkpd fpdaycnlah clqivcdwtd yearmkklvs
      541 ivaeqldknr lpsvhphhsm lyplshefrk aiaarhanlc iekihvlhkq pykyprevgt
      601 rlrigyvssd fgnhptshlm qsipglhdrq tveifcyals addgttfrak iareaehfid
      661 lsqipcngka adrinadgvh ilvnmngytk garneifalr papvqvmwlg ypgtsgasfm
      721 dylitdevts pvelasqyse klaymphtyf igdhrqmfph lkerliltdk lntkgkvadn
      781 vavinatdls pmientcvke irevvvpdak nkpveislkv aelstttaie tmiasgqcqm
      841 svngvvvqng mattqvnnkt atgeevpqni vittrqqygl pedavvycnf nqlykidplt
      901 lhmwahilkh vpnsvlwllr fpavgepnlq ataqqlgltp grilfsnvaa keehvrrgql
      961 advcldtplc nghttsmdvl wtgtpvvtlp getlasrvaa sqlntlgcpe liartrqeyq
     1021 diairlgtdr eylkatrakv wkarsesplf ncklyalgme mlykkmwery srgekpdhva
     1081 avekndrdkl mits
//
LOCUS       XP_034189306            1064 aa            linear   INV 03-SEP-2025
DEFINITION  O-linked N-acetylglucosamine (GlcNAc) transferase sxc isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034189306
VERSION     XP_034189306.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333415.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1064
                     /product="O-linked N-acetylglucosamine (GlcNAc)
                     transferase sxc isoform X2"
                     /calculated_mol_wt=119156
     Region          43..68
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          70..308
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          76..102
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          108..136
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(109,112..113,116..117,119,143,146..147,150..151,
                     153..154,177,180..181,184..185,188)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          141..171
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          176..204
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          209..476
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          210..238
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(245,248..249,252..253,255,279,282..283,286..287,
                     289..290,313,316..317,320..321,324)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          245..272
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          277..307
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          312..340
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          346..374
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          380..408
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(381,384..385,388..389,391,415,418..419,422..423,
                     425..426,449,452..453,456..457,460)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          413..443
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          448..476
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          495..1029
                     /region_name="Glyco_transf_41"
                     /note="Glycosyl transferase family 41; pfam13844"
                     /db_xref="CDD:404688"
     CDS             1..1064
                     /gene="sxc"
                     /coded_by="XM_034333415.2:343..3537"
                     /db_xref="GeneID:117608373"
ORIGIN      
        1 mvtvimqaqq qpqqqqqqnh qqiagtsvil kmneiqqlst vgllelahre yqagdyenae
       61 rhcmqlwrqe tnntgvllll ssihfqcrrl eksahyssla ikqnpllaea ysnlgnvfke
      121 rgqlqealen yrhavrlkpd fidgyinlaa alvaagdmeq avqayvtalq ynpdlycvrs
      181 dlgnllkala rldeakacyl kaietrpdfa vawsnlgcvf naqgeiwlai hhfekavald
      241 pnfldayinl gnvlkearif dravaaylra lnlspnnavv hgnlacvyye qglidlaidt
      301 yrraielqpn fpdaycnlan alkekgqvve aedcyntalr lcpshadsln nlanikreqg
      361 yieeatrlyl kalevfpefa aahsnlasvl qqqgklneal mhykeairiq ptfadaysnm
      421 gntlkemqdi qgalqcytra iqinpafada hsnlasihkd sgnipeaiqs yrtalklkpd
      481 fpdaycnlah clqivcdwtd yearmkklvs ivaeqldknr lpsvhphhsm lyplshefrk
      541 aiaarhanlc iekihvlhkq pykyprevgt rlrigyvssd fgnhptshlm qsipglhdrq
      601 tveifcyals addgttfrak iareaehfid lsqipcngka adrinadgvh ilvnmngytk
      661 garneifalr papvqvmwlg ypgtsgasfm dylitdevts pvelasqyse klaymphtyf
      721 igdhrqmfph lkerliltdk lntkgkvadn vavinatdls pmientcvke irevvvpdak
      781 nkpveislkv aelstttaie tmiasgqcqm svngvvvqng mattqvnnkt atgeevpqni
      841 vittrqqygl pedavvycnf nqlykidplt lhmwahilkh vpnsvlwllr fpavgepnlq
      901 ataqqlgltp grilfsnvaa keehvrrgql advcldtplc nghttsmdvl wtgtpvvtlp
      961 getlasrvaa sqlntlgcpe liartrqeyq diairlgtdr eylkatrakv wkarsesplf
     1021 ncklyalgme mlykkmwery srgekpdhva avekndrdkl mits
//
LOCUS       XP_034189327             872 aa            linear   INV 03-SEP-2025
DEFINITION  FIGNL1-interacting regulator of recombination and mitosis [Osmia
            lignaria lignaria].
ACCESSION   XP_034189327
VERSION     XP_034189327.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333436.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189327.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..872
                     /product="FIGNL1-interacting regulator of recombination
                     and mitosis"
                     /calculated_mol_wt=99534
     Region          <347..>516
                     /region_name="DUF4487"
                     /note="Domain of unknown function (DUF4487); pfam14868"
                     /db_xref="CDD:464352"
     CDS             1..872
                     /gene="LOC117608382"
                     /coded_by="XM_034333436.2:145..2763"
                     /db_xref="GeneID:117608382"
ORIGIN      
        1 madensfnsl ledsftdldi shedyisklk daveklrern gvesfqlalq gylsddsdny
       61 snvltfnkil ppvhhflsei lkeitgiihg naedkmgnvk rkllmchell kieeismhrm
      121 aqfqersass fksisnnlll siklvfehcr eskklyetlf eavekelasl frkaktnltl
      181 ftvilnsvmv fdtdkesete llikvidsig slmtishdls phtfvetsks fcdmaikhql
      241 avkrinvesv tshlvemtkd virtlslfqd ssnpieersi kvvgrmlkil dklfatyssd
      301 inneillrvi eillqmhrcy plceketqtd srtitllnlh isrsvepfln tvfetphfkq
      361 affeygnqtk idrlgyhllt isimkklisi pykqhcnwtl gsesiidiaf tnindiqeei
      421 cmgqikiqga heigerpeka slyeatlvpi cclisqipad gfhamelill khllnnrlws
      481 smlssdvwcf igrigsselc ashvkyllrv yaalmkrnns feviilenli grlysllaee
      541 trhnilmele dlenpswipl arflpsktkl flqnqlacvl neipstfiel qrqptvqnwn
      601 rimtltsmig klnyagekst idilsqiwns vantienceg rqldilsefv sklfnamese
      661 tiqddmfvai leavltsfpc fpphvkvias hylrnsinff gncmvrssya laelncrlle
      721 denpwvrqea lesfdhvahm cpnedlvttm aaavtkkasl ndtlpaylsg ttylelqdfs
      781 dvkhylqhva rnslnichvc cnyedsqkee klarlenqsv enpsvndldk hvnkicdeln
      841 diikkqdeis ndalqkllst cieiadliks tk
//
LOCUS       XP_076546121             872 aa            linear   INV 03-SEP-2025
DEFINITION  FIGNL1-interacting regulator of recombination and mitosis [Osmia
            lignaria lignaria].
ACCESSION   XP_076546121
VERSION     XP_076546121.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690006.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..872
                     /product="FIGNL1-interacting regulator of recombination
                     and mitosis"
                     /calculated_mol_wt=99534
     Region          <347..>516
                     /region_name="DUF4487"
                     /note="Domain of unknown function (DUF4487); pfam14868"
                     /db_xref="CDD:464352"
     CDS             1..872
                     /gene="LOC117608382"
                     /coded_by="XM_076690006.1:243..2861"
                     /db_xref="GeneID:117608382"
ORIGIN      
        1 madensfnsl ledsftdldi shedyisklk daveklrern gvesfqlalq gylsddsdny
       61 snvltfnkil ppvhhflsei lkeitgiihg naedkmgnvk rkllmchell kieeismhrm
      121 aqfqersass fksisnnlll siklvfehcr eskklyetlf eavekelasl frkaktnltl
      181 ftvilnsvmv fdtdkesete llikvidsig slmtishdls phtfvetsks fcdmaikhql
      241 avkrinvesv tshlvemtkd virtlslfqd ssnpieersi kvvgrmlkil dklfatyssd
      301 inneillrvi eillqmhrcy plceketqtd srtitllnlh isrsvepfln tvfetphfkq
      361 affeygnqtk idrlgyhllt isimkklisi pykqhcnwtl gsesiidiaf tnindiqeei
      421 cmgqikiqga heigerpeka slyeatlvpi cclisqipad gfhamelill khllnnrlws
      481 smlssdvwcf igrigsselc ashvkyllrv yaalmkrnns feviilenli grlysllaee
      541 trhnilmele dlenpswipl arflpsktkl flqnqlacvl neipstfiel qrqptvqnwn
      601 rimtltsmig klnyagekst idilsqiwns vantienceg rqldilsefv sklfnamese
      661 tiqddmfvai leavltsfpc fpphvkvias hylrnsinff gncmvrssya laelncrlle
      721 denpwvrqea lesfdhvahm cpnedlvttm aaavtkkasl ndtlpaylsg ttylelqdfs
      781 dvkhylqhva rnslnichvc cnyedsqkee klarlenqsv enpsvndldk hvnkicdeln
      841 diikkqdeis ndalqkllst cieiadliks tk
//
LOCUS       XP_034189349             838 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608396 [Osmia lignaria lignaria].
ACCESSION   XP_034189349
VERSION     XP_034189349.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333458.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189349.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="uncharacterized protein LOC117608396"
                     /calculated_mol_wt=94972
     CDS             1..838
                     /gene="LOC117608396"
                     /coded_by="XM_034333458.2:174..2690"
                     /db_xref="GeneID:117608396"
ORIGIN      
        1 msesgksvrp vrqlslqpaa prrqfirrqr saeeekslqi cvsslargkr gtnakpnser
       61 pkvriawken rnqnereveq vevvarqipg rsrpatgrsr gfiaidkpsi lcsrqelaer
      121 lrlawkhree skaninifla hktledrcds emsshatvta psspeskyki sfsensrrip
      181 deviripntr trsagqsmnk etrndlllsf ersnvgeiam lnvnkiaenk kkmgkencev
      241 ekeqidkeil rnantegyle nskkktsmii dcstsrvpsk asplikiens plevekvske
      301 dfssakqkra sfrsgtnraf lgpiklptne leknrteske nvtnkpvitk svsdkivikt
      361 tgdckakeni leskdssayn kmmiqktndv dkcnltikrt vsstvddnsm idkvlttkns
      421 mevrcssvne rnpplkkiei qriderprrt ssappqrhve tnssnriqvn ividtavngt
      481 sgkgreqdkl emdrrnsgie rrdttkiagr svrsaplkrr srsarrrywg sgktdeegks
      541 rhrssgrgrn sidsrtidiv tmvslvssad sdsdienspr ddklinelrs klpttsiikt
      601 smnplcsark piksvsfqkd sfdedsskeq qpqvkeekkt tqprlaivsh rgnggtlssn
      661 eetvswrtei sdvtvpvltl ihdaeetldv pltdrekrcl avpiadlrdk krkllktrst
      721 ssrleiekqt llsktkiqes privqqkmei prqqiktptn nitanvksif vshsketvqp
      781 vqstskeprf qtnkekecwh lyrkmcdkgi cvsfdtvlrg mltpteyrlr qkelsqdl
//
LOCUS       XP_076546122             562 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546122
VERSION     XP_076546122.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690007.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..562
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X1"
                     /calculated_mol_wt=63506
     Region          92..218
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          233..483
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..562
                     /gene="shaker"
                     /coded_by="XM_076690007.1:5481..7169"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mwqsggmgah aannpwmklm givhkerrhh dsssaaaasg vqgsgttgsn drtlnqslpk
       61 lssqdedgpn phtqytgvth fepiphdhdf cervvinvsg lkfetqlrtl nqfpdtllgd
      121 psrrlryfdp lrneyffdrn rpsfdailyy yqsggrlrrp vnvpldvfse eikfyelgel
      181 atnkfredeg fikeeekplp shefqrkvwl lfeypessqg arvvaiisvv villsivifc
      241 letlpefkhy kvfntttngt kieedevpdi tdpfflieti ciiwftfels vrflacpnkl
      301 nffrdvmnii diiaiipyfi tlatvvaeee etidlpkapv spqdkstnqa mslailrvir
      361 lvrvfrifkl srhskglqil grtlkasmre lgllifflfi gvvlfssavy faeagsensf
      421 fksipdafww avvtmttvgy gdmtpvgvwg kivgslcaia gvltialpvp vivsnfnyfy
      481 hretdqeemq sqnfnhvtsc pylpgtlgqh vkkspmsess sdimeleegi llphpeitkk
      541 pncnprhnnn inpacmsiet dv
//
LOCUS       XP_034189392             562 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189392
VERSION     XP_034189392.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333501.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..562
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X1"
                     /calculated_mol_wt=63506
     Region          92..218
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          233..483
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..562
                     /gene="shaker"
                     /coded_by="XM_034333501.2:1007..2695"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mwqsggmgah aannpwmklm givhkerrhh dsssaaaasg vqgsgttgsn drtlnqslpk
       61 lssqdedgpn phtqytgvth fepiphdhdf cervvinvsg lkfetqlrtl nqfpdtllgd
      121 psrrlryfdp lrneyffdrn rpsfdailyy yqsggrlrrp vnvpldvfse eikfyelgel
      181 atnkfredeg fikeeekplp shefqrkvwl lfeypessqg arvvaiisvv villsivifc
      241 letlpefkhy kvfntttngt kieedevpdi tdpfflieti ciiwftfels vrflacpnkl
      301 nffrdvmnii diiaiipyfi tlatvvaeee etidlpkapv spqdkstnqa mslailrvir
      361 lvrvfrifkl srhskglqil grtlkasmre lgllifflfi gvvlfssavy faeagsensf
      421 fksipdafww avvtmttvgy gdmtpvgvwg kivgslcaia gvltialpvp vivsnfnyfy
      481 hretdqeemq sqnfnhvtsc pylpgtlgqh vkkspmsess sdimeleegi llphpeitkk
      541 pncnprhnnn inpacmsiet dv
//
LOCUS       XP_076546123             562 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546123
VERSION     XP_076546123.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690008.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..562
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X2"
                     /calculated_mol_wt=63575
     Region          92..218
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          233..483
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..562
                     /gene="shaker"
                     /coded_by="XM_076690008.1:5483..7171"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mwqsggmgah aannpwmklm givhkerrhh dsssaaaasg vqgsgttgsn drtlnqslpk
       61 lssqdedgpn phtqytgvth fepiphdhdf cervvinvsg lkfetqlrtl nqfpdtllgd
      121 psrrlryfdp lrneyffdrn rpsfdailyy yqsggrlrrp vnvpldvfse eikfyelgel
      181 atnkfredeg fikeeekplp shefqrkvwl lfeypessqg arvvaiisvv villsivifc
      241 letlpefkhy kvfntttngt kieedevpdi tdpfflieti ciiwftfels vrflacpnkl
      301 nffrdvmnii diiaiipyfi tlatvvaeee etidlpkapv spqdkstnqa mslailrvir
      361 lvrvfrifkl srhskglqil grtlkasmre lgllifflfi gvvlfssavy faeagtqdsf
      421 fksipdafww avvtmttvgy gdmrpvgvwg kivgslcaia gvltialpvp vivsnfnyfy
      481 hretdqeemq sqnfnhvtsc pylpgtlgqh vkkspmsess sdimeleegi llphpeitkk
      541 pncnprhnnn inpacmsiet dv
//
LOCUS       XP_034189402             553 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189402
VERSION     XP_034189402.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333511.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X3"
                     /calculated_mol_wt=62683
     Region          83..209
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          224..474
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..553
                     /gene="shaker"
                     /coded_by="XM_034333511.2:2636..4297"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mglgsllvhs viliivsnli lrsigicyic lpyslvltvf vssalyvslp klssqdedgp
       61 nphtqytgvt hfepiphdhd fcervvinvs glkfetqlrt lnqfpdtllg dpsrrlryfd
      121 plrneyffdr nrpsfdaily yyqsggrlrr pvnvpldvfs eeikfyelge latnkfrede
      181 gfikeeekpl pshefqrkvw llfeypessq garvvaiisv vvillsivif cletlpefkh
      241 ykvfntttng tkieedevpd itdpffliet iciiwftfel svrflacpnk lnffrdvmni
      301 idiiaiipyf itlatvvaee eetidlpkap vspqdkstnq amslailrvi rlvrvfrifk
      361 lsrhskglqi lgrtlkasmr elgllifflf igvvlfssav yfaeagsens ffksipdafw
      421 wavvtmttvg ygdmtpvgvw gkivgslcai agvltialpv pvivsnfnyf yhretdqeem
      481 qsqnfnhvts cpylpgtlgq hvkkspmses ssdimeleeg illphpeitk kpncnprhnn
      541 ninpacmsie tdv
//
LOCUS       XP_034189411             539 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189411
VERSION     XP_034189411.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333520.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..539
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X4"
                     /calculated_mol_wt=61493
     Region          69..195
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          210..460
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..539
                     /gene="shaker"
                     /coded_by="XM_034333520.2:1063..2682"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mtmwierllf slvilpgggq gdhrsqlprr kkrslpklss qdedgpnpht qytgvthfep
       61 iphdhdfcer vvinvsglkf etqlrtlnqf pdtllgdpsr rlryfdplrn eyffdrnrps
      121 fdailyyyqs ggrlrrpvnv pldvfseeik fyelgelatn kfredegfik eeekplpshe
      181 fqrkvwllfe ypessqgarv vaiisvvvil lsivifclet lpefkhykvf ntttngtkie
      241 edevpditdp fflieticii wftfelsvrf lacpnklnff rdvmniidii aiipyfitla
      301 tvvaeeeeti dlpkapvspq dkstnqamsl ailrvirlvr vfrifklsrh skglqilgrt
      361 lkasmrelgl lifflfigvv lfssavyfae agsensffks ipdafwwavv tmttvgygdm
      421 tpvgvwgkiv gslcaiagvl tialpvpviv snfnyfyhre tdqeemqsqn fnhvtscpyl
      481 pgtlgqhvkk spmsesssdi meleegillp hpeitkkpnc nprhnnninp acmsietdv
//
LOCUS       XP_034189421             539 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189421
VERSION     XP_034189421.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333530.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..539
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X4"
                     /calculated_mol_wt=61493
     Region          69..195
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          210..460
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..539
                     /gene="shaker"
                     /coded_by="XM_034333530.2:182..1801"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mtmwierllf slvilpgggq gdhrsqlprr kkrslpklss qdedgpnpht qytgvthfep
       61 iphdhdfcer vvinvsglkf etqlrtlnqf pdtllgdpsr rlryfdplrn eyffdrnrps
      121 fdailyyyqs ggrlrrpvnv pldvfseeik fyelgelatn kfredegfik eeekplpshe
      181 fqrkvwllfe ypessqgarv vaiisvvvil lsivifclet lpefkhykvf ntttngtkie
      241 edevpditdp fflieticii wftfelsvrf lacpnklnff rdvmniidii aiipyfitla
      301 tvvaeeeeti dlpkapvspq dkstnqamsl ailrvirlvr vfrifklsrh skglqilgrt
      361 lkasmrelgl lifflfigvv lfssavyfae agsensffks ipdafwwavv tmttvgygdm
      421 tpvgvwgkiv gslcaiagvl tialpvpviv snfnyfyhre tdqeemqsqn fnhvtscpyl
      481 pgtlgqhvkk spmsesssdi meleegillp hpeitkkpnc nprhnnninp acmsietdv
//
LOCUS       XP_034189430             534 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189430
VERSION     XP_034189430.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333539.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X5"
                     /calculated_mol_wt=60990
     Region          64..190
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          205..455
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..534
                     /gene="shaker"
                     /coded_by="XM_034333539.2:260..1864"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mhagmrgafl gmrmlprrrr ksserktrsl pklssqdedg pnphtqytgv thfepiphdh
       61 dfcervvinv sglkfetqlr tlnqfpdtll gdpsrrlryf dplrneyffd rnrpsfdail
      121 yyyqsggrlr rpvnvpldvf seeikfyelg elatnkfred egfikeeekp lpshefqrkv
      181 wllfeypess qgarvvaiis vvvillsivi fcletlpefk hykvfntttn gtkieedevp
      241 ditdpfflie ticiiwftfe lsvrflacpn klnffrdvmn iidiiaiipy fitlatvvae
      301 eeetidlpka pvspqdkstn qamslailrv irlvrvfrif klsrhskglq ilgrtlkasm
      361 relglliffl figvvlfssa vyfaeagsen sffksipdaf wwavvtmttv gygdmtpvgv
      421 wgkivgslca iagvltialp vpvivsnfny fyhretdqee mqsqnfnhvt scpylpgtlg
      481 qhvkkspmse sssdimelee gillphpeit kkpncnprhn nninpacmsi etdv
//
LOCUS       XP_076546124             534 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546124
VERSION     XP_076546124.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690009.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X6"
                     /calculated_mol_wt=61059
     Region          64..190
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          205..455
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..534
                     /gene="shaker"
                     /coded_by="XM_076690009.1:264..1868"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mhagmrgafl gmrmlprrrr ksserktrsl pklssqdedg pnphtqytgv thfepiphdh
       61 dfcervvinv sglkfetqlr tlnqfpdtll gdpsrrlryf dplrneyffd rnrpsfdail
      121 yyyqsggrlr rpvnvpldvf seeikfyelg elatnkfred egfikeeekp lpshefqrkv
      181 wllfeypess qgarvvaiis vvvillsivi fcletlpefk hykvfntttn gtkieedevp
      241 ditdpfflie ticiiwftfe lsvrflacpn klnffrdvmn iidiiaiipy fitlatvvae
      301 eeetidlpka pvspqdkstn qamslailrv irlvrvfrif klsrhskglq ilgrtlkasm
      361 relglliffl figvvlfssa vyfaeagtqd sffksipdaf wwavvtmttv gygdmrpvgv
      421 wgkivgslca iagvltialp vpvivsnfny fyhretdqee mqsqnfnhvt scpylpgtlg
      481 qhvkkspmse sssdimelee gillphpeit kkpncnprhn nninpacmsi etdv
//
LOCUS       XP_034189440             531 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189440
VERSION     XP_034189440.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333549.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..531
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X7"
                     /calculated_mol_wt=60502
     Region          61..187
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          202..452
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..531
                     /gene="shaker"
                     /coded_by="XM_034333549.2:2660..4255"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 maavagiygf aedrhvrhte hrqhpslpkl ssqdedgpnp htqytgvthf epiphdhdfc
       61 ervvinvsgl kfetqlrtln qfpdtllgdp srrlryfdpl rneyffdrnr psfdailyyy
      121 qsggrlrrpv nvpldvfsee ikfyelgela tnkfredegf ikeeekplps hefqrkvwll
      181 feypessqga rvvaiisvvv illsivifcl etlpefkhyk vfntttngtk ieedevpdit
      241 dpfflietic iiwftfelsv rflacpnkln ffrdvmniid iiaiipyfit latvvaeeee
      301 tidlpkapvs pqdkstnqam slailrvirl vrvfrifkls rhskglqilg rtlkasmrel
      361 gllifflfig vvlfssavyf aeagsensff ksipdafwwa vvtmttvgyg dmtpvgvwgk
      421 ivgslcaiag vltialpvpv ivsnfnyfyh retdqeemqs qnfnhvtscp ylpgtlgqhv
      481 kkspmsesss dimeleegil lphpeitkkp ncnprhnnni npacmsietd v
//
LOCUS       XP_034189448             515 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189448
VERSION     XP_034189448.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333557.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X8"
                     /calculated_mol_wt=58535
     Region          45..171
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          186..436
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..515
                     /gene="shaker"
                     /coded_by="XM_034333557.2:1749..3296"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mqmilvaggs lpklssqded gpnphtqytg vthfepiphd hdfcervvin vsglkfetql
       61 rtlnqfpdtl lgdpsrrlry fdplrneyff drnrpsfdai lyyyqsggrl rrpvnvpldv
      121 fseeikfyel gelatnkfre degfikeeek plpshefqrk vwllfeypes sqgarvvaii
      181 svvvillsiv ifcletlpef khykvfnttt ngtkieedev pditdpffli eticiiwftf
      241 elsvrflacp nklnffrdvm niidiiaiip yfitlatvva eeeetidlpk apvspqdkst
      301 nqamslailr virlvrvfri fklsrhskgl qilgrtlkas mrelglliff lfigvvlfss
      361 avyfaeagse nsffksipda fwwavvtmtt vgygdmtpvg vwgkivgslc aiagvltial
      421 pvpvivsnfn yfyhretdqe emqsqnfnhv tscpylpgtl gqhvkkspms esssdimele
      481 egillphpei tkkpncnprh nnninpacms ietdv
//
LOCUS       XP_076546125             515 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546125
VERSION     XP_076546125.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690010.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X8"
                     /calculated_mol_wt=58535
     Region          45..171
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          186..436
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..515
                     /gene="shaker"
                     /coded_by="XM_076690010.1:1450..2997"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mqmilvaggs lpklssqded gpnphtqytg vthfepiphd hdfcervvin vsglkfetql
       61 rtlnqfpdtl lgdpsrrlry fdplrneyff drnrpsfdai lyyyqsggrl rrpvnvpldv
      121 fseeikfyel gelatnkfre degfikeeek plpshefqrk vwllfeypes sqgarvvaii
      181 svvvillsiv ifcletlpef khykvfnttt ngtkieedev pditdpffli eticiiwftf
      241 elsvrflacp nklnffrdvm niidiiaiip yfitlatvva eeeetidlpk apvspqdkst
      301 nqamslailr virlvrvfri fklsrhskgl qilgrtlkas mrelglliff lfigvvlfss
      361 avyfaeagse nsffksipda fwwavvtmtt vgygdmtpvg vwgkivgslc aiagvltial
      421 pvpvivsnfn yfyhretdqe emqsqnfnhv tscpylpgtl gqhvkkspms esssdimele
      481 egillphpei tkkpncnprh nnninpacms ietdv
//
LOCUS       XP_076546126             419 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X9 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546126
VERSION     XP_076546126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690011.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..419
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X9"
                     /calculated_mol_wt=47764
     Region          92..218
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          233..>400
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..419
                     /gene="shaker"
                     /coded_by="XM_076690011.1:5490..6749"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mwqsggmgah aannpwmklm givhkerrhh dsssaaaasg vqgsgttgsn drtlnqslpk
       61 lssqdedgpn phtqytgvth fepiphdhdf cervvinvsg lkfetqlrtl nqfpdtllgd
      121 psrrlryfdp lrneyffdrn rpsfdailyy yqsggrlrrp vnvpldvfse eikfyelgel
      181 atnkfredeg fikeeekplp shefqrkvwl lfeypessqg arvvaiisvv villsivifc
      241 letlpefkhy kvfntttngt kieedevpdi tdpfflieti ciiwftfels vrflacpnkl
      301 nffrdvmnii diiaiipyfi tlatvvaeee etidlpkapv spqdkstnqa mslailrvir
      361 lvrvfrifkl srhskglqil grtlkasmre lgllifflfi grleyggrss glcarslvy
//
LOCUS       XP_076546127             409 aa            linear   INV 03-SEP-2025
DEFINITION  potassium voltage-gated channel protein Shaker isoform X10 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546127
VERSION     XP_076546127.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690012.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="potassium voltage-gated channel protein Shaker
                     isoform X10"
                     /calculated_mol_wt=46799
     Region          92..218
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          233..>400
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..409
                     /gene="shaker"
                     /coded_by="XM_076690012.1:5490..6719"
                     /db_xref="GeneID:117608403"
ORIGIN      
        1 mwqsggmgah aannpwmklm givhkerrhh dsssaaaasg vqgsgttgsn drtlnqslpk
       61 lssqdedgpn phtqytgvth fepiphdhdf cervvinvsg lkfetqlrtl nqfpdtllgd
      121 psrrlryfdp lrneyffdrn rpsfdailyy yqsggrlrrp vnvpldvfse eikfyelgel
      181 atnkfredeg fikeeekplp shefqrkvwl lfeypessqg arvvaiisvv villsivifc
      241 letlpefkhy kvfntttngt kieedevpdi tdpfflieti ciiwftfels vrflacpnkl
      301 nffrdvmnii diiaiipyfi tlatvvaeee etidlpkapv spqdkstnqa mslailrvir
      361 lvrvfrifkl srhskglqil grtlkasmre lgllifflfi akkklkqvh
//
LOCUS       XP_034189483             708 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase LRSAM1 [Osmia lignaria lignaria].
ACCESSION   XP_034189483
VERSION     XP_034189483.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333592.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="E3 ubiquitin-protein ligase LRSAM1"
                     /calculated_mol_wt=80818
     Region          57..72
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          <87..>192
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          105..127
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          128..150
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          151..177
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          245..>624
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          657..703
                     /region_name="RING-HC_LRSAM1"
                     /note="RING finger, HC subclass, found in leucine-rich
                     repeat and sterile alpha motif-containing protein 1
                     (LRSAM1) and similar proteins; cd16515"
                     /db_xref="CDD:438178"
     CDS             1..708
                     /gene="LOC117608445"
                     /coded_by="XM_034333592.2:7..2133"
                     /db_xref="GeneID:117608445"
ORIGIN      
        1 mslprkhgnk vnvdykarle hklylarenp epifdvseca lkhvpsgiys lckvfrkkvl
       61 wmysnkltsl sgggalsdls llvvldihgn eftnlppdim clvslkelyl qdnnirklpn
      121 eivhlsklni lnvsrnnlkq lpetigtlrq litldisqnk slhklpkslg yaqqlaqlni
      181 dglnlsyppq dilyggtvvi iaflanengi eylpeesvsd velskntssd dtqgtynknh
      241 dvqaalqkla kmkehrqnal leveknmkqq qeyeigihsm lkehrkklle dlaleqsqll
      301 delqkvqqer nsnsarllsy iynaekeann vikeflrnse qerqsqaell krekqeeiql
      361 llschseqfm frtkdtllsm eklleeelhr trkleeyskh rdyaaqsllt levrnndhla
      421 qivqsqernr qdlvdtlkqd evlqkaavaa llersdarsw simqqvnlvq lqlaaltnie
      481 lekrklemnq qlneiaekrv alsailmnll eqqksrrnql letinniehq rtinnsrrns
      541 qfwlmqyqsl mearpqglle mldpmlvrhv aiagalhylp flaslpsllp dltdeqleti
      601 gihceadraa irlavenyla eiklnehrtp vtpsappeea ctssnyeesn piqsintaec
      661 vicldlqcev iflpcghlcc csgcanmvss dcpmcrstie hkihvikp
//
LOCUS       XP_034189517             435 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: small ribonucleoprotein particle U1 subunit
            70K [Osmia lignaria lignaria].
ACCESSION   XP_034189517
VERSION     XP_034189517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333626.2
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..435
                     /product="LOW QUALITY PROTEIN: small ribonucleoprotein
                     particle U1 subunit 70K"
                     /calculated_mol_wt=52063
     Region          3..91
                     /region_name="U1snRNP70_N"
                     /note="U1 small nuclear ribonucleoprotein of 70kDa MW N
                     terminal; pfam12220"
                     /db_xref="CDD:432406"
     Region          101..213
                     /region_name="RRM_snRNP70"
                     /note="RNA recognition motif (RRM) found in U1 small
                     nuclear ribonucleoprotein 70 kDa (U1-70K) and similar
                     proteins; cd12236"
                     /db_xref="CDD:409682"
     CDS             1..435
                     /gene="snRNP-U1-70K"
                     /coded_by="XM_034333626.2:141..1448"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon"
                     /db_xref="GeneID:117608461"
ORIGIN      
        1 mtqflppnll alfaprdpip ylppvsklph ekknggyigv ggflkyfedp kdtpppvrve
       61 treerlerrr reraeqvayk leqeiavwdp agipnvtadp fktlfvarin ydtsesklrr
      121 efevygpvkk ivvihnting kprgyafiey eherdmhsww plpatsavhy smqxslnlpd
      181 miaaykhadg kkidgrrvlv dverartvkg wlprrlgggl ggtrrggpdv nikhsgredn
      241 erereryrle rerensgrld rdrdrdrldr errrssrdrk rrtrsrsrdr krrrsrdrlp
      301 dpdieeiqre erprerrdre rdrdrdrdrk rrrsrsndrd rdrdrkrdkr drdrerrdrk
      361 drgdrqeedt keirikeepl ddypdysntf stsgsyftav kyeedndqev eekyripegr
      421 pdpppynnyd svpdy
//
LOCUS       XP_034189554             383 aa            linear   INV 03-SEP-2025
DEFINITION  gamma-soluble NSF attachment protein-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034189554
VERSION     XP_034189554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333663.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="gamma-soluble NSF attachment protein-like"
                     /calculated_mol_wt=42049
     Region          6..244
                     /region_name="SNAP"
                     /note="Soluble N-ethylmaleimide-sensitive factor (NSF)
                     Attachment Protein family; cl24038"
                     /db_xref="CDD:451671"
     Region          28..65
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     Region          69..106
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     Region          109..144
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     Region          148..184
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276937"
     Region          <272..369
                     /region_name="Metaviral_G"
                     /note="Metaviral_G glycoprotein; pfam09595"
                     /db_xref="CDD:462833"
     CDS             1..383
                     /gene="LOC117608473"
                     /coded_by="XM_034333663.2:191..1342"
                     /db_xref="GeneID:117608473"
ORIGIN      
        1 mskieegnah irqaekslkt sllkwrpdfe vaadeyttaa tcfriaksyk qckdclmkas
       61 dcykenrawf haaksieqal lickemgnls evpklahsac slyqmhgspe sgatvldkag
      121 kmmeatqpqe alelfkraan ivmgedsprq aaeymskvar llvklqmyde aadavrreig
      181 mhqqidhaps vgrltvalvl vqlargdqva aekafkewgn yceapevntl emllqaydne
      241 dadaaraaln spfikhmdve yaklarglpl pqqeytmppp gvranaaesy tspnasklie
      301 ettseiqnms vktsevaaki psednaatss editktssed ttetlsenta tsskdtvtkk
      361 etpeapsqdv ekededdyeg glc
//
LOCUS       XP_076546128             203 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305651 [Osmia lignaria lignaria].
ACCESSION   XP_076546128
VERSION     XP_076546128.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690013.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..203
                     /product="uncharacterized protein LOC143305651"
                     /calculated_mol_wt=21639
     CDS             1..203
                     /gene="LOC143305651"
                     /coded_by="XM_076690013.1:4530..5141"
                     /db_xref="GeneID:143305651"
ORIGIN      
        1 mggpgagrvs rhelnavpks hvghgdrtlv igeptaptap tttscrchst seraisvsgl
       61 vpaqlppspp plaslhivdn scplignntt ttnnnnntnt saspnalssg tkaattmssp
      121 pkhrrgfdpn dvnandyrry rrvktrrgfv cstrwvtlga ghrpprhlpt yicqhealkn
      181 thklamfdvg plltgcipdt kmr
//
LOCUS       XP_034189602             129 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase epsilon subunit 3 Chrac-14 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189602
VERSION     XP_034189602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..129
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..129
                     /product="DNA polymerase epsilon subunit 3 Chrac-14"
                     /calculated_mol_wt=14300
     Region          6..92
                     /region_name="HFD_POLE3_DPB4"
                     /note="histone-fold domain found in DNA polymerase epsilon
                     subunit 3 (POLE3) and similar proteins; cd22928"
                     /db_xref="CDD:467053"
     Site            order(6..7,39,43,46..47,50..51,66..67,75,80,86..87,90)
                     /site_type="other"
                     /note="catalytic subunit binding site [polypeptide
                     binding]"
                     /db_xref="CDD:467053"
     Site            order(14,18..19,21,29..30,32..33,36..37,39..41,43..49,
                     62..66,69,76..78,81,84..85,88..89,91..92)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467053"
     CDS             1..129
                     /gene="Chrac-14"
                     /coded_by="XM_034333711.2:377..766"
                     /db_xref="GeneID:117608492"
ORIGIN      
        1 maerledlnl pnavvtriik ealpegvtva kdartavaka ssifilylts saniiakkgn
       61 rktisgsdvi qamtdiefdq fieplqesle nfrkaqkekk datskkkqqk kdeddvieee
      121 dggrevmvy
//
LOCUS       XP_034171756             679 aa            linear   INV 03-SEP-2025
DEFINITION  dopamine D2-like receptor [Osmia lignaria lignaria].
ACCESSION   XP_034171756
VERSION     XP_034171756.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171756.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..679
                     /product="dopamine D2-like receptor"
                     /calculated_mol_wt=73379
     Region          180..>369
                     /region_name="7tmA_D2-like_dopamine_R"
                     /note="D2-like dopamine receptors, member of the class A
                     family of seven-transmembrane G protein-coupled receptors;
                     cd15053"
                     /db_xref="CDD:320181"
     Region          180..206
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320181"
     Region          213..239
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320181"
     Region          251..281
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320181"
     Region          293..316
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320181"
     Region          332..361
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320181"
     Region          <597..673
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          603..625
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          641..666
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..679
                     /gene="Dop2R"
                     /coded_by="XM_034315865.2:1601..3640"
                     /db_xref="GeneID:117600413"
ORIGIN      
        1 masktnesfv rsilrnlsld qtqrptdmft vngtkdagiv rdeeialndv lsksdkddss
       61 sgielgwynd sttiastlsg flfgvpssss sssslfsssv ssenytgisd lfvfedlndy
      121 inrlnysafa nltayydgga nssvngsvnc tssivagggv arvndcaaad vdektnansw
      181 walilvivpc ltlfgnvlvi lavvreralq tvtnyfivsl avadllvavl vmpfavyvlv
      241 ngswslpgfv cdfyiamdvt cstssifnlv aisidryiav tqpikyakhk nnrrvwltil
      301 lvwaisaaig spivlglnnt pdripdqclf ynadfiiyss lssfyipcii mvflyynifk
      361 alrnrakkar anrkpnlgdi kpgsiienia htrrfaetal gaaalvapgm eeptntasgs
      421 nededetpld pvvvisndks tefflatvve eaacrfsava qaqlggtqha rkdsgyegaa
      481 sttlaqeqle tnsspspnpr iasapsssts sspppksaiv sqtsqskkng aetnkqelkr
      541 lksasallpl qlgrtpsvls asstckkdkk naaagsrfti ykankaskkk rekssakker
      601 katktlaivl gvflicwlpf ftcnimdalc tklqancqpg vtafivtswl gymnsfvnpv
      661 iytvfnpefr kafhklvsf
//
LOCUS       XP_034195417             384 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611559 [Osmia lignaria lignaria].
ACCESSION   XP_034195417
VERSION     XP_034195417.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339526.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195417.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="uncharacterized protein LOC117611559"
                     /calculated_mol_wt=44682
     CDS             1..384
                     /gene="LOC117611559"
                     /coded_by="XM_034339526.2:206..1360"
                     /db_xref="GeneID:117611559"
ORIGIN      
        1 metlwflffv wcttmvtsfd lgpiiriaqc rsqclrkhtt dgvcdwysgq qqtscsmcwq
       61 yceglenrwe ktrticegdq ylhcpacqtt cnyrktrvee kylpsmlpap kkapidldrf
      121 dvavvmrkvy kqwrvtgyys gghnpnlrpd twiivamedg vkhyswqewv pkleslkegp
      181 lyeatiswkd vqtqlqkqrs leqvrfnnrv rqfflekyge kvlaewrsqe dsqisdevfr
      241 rfffrrkddr nedsmetdnt pstsndyekl dkdqgnresy vvswepetgg lmgnqvadsn
      301 saqislvpgt kyfvriasnd gpgsfpievd trpnfvqakr iertleylyp wdvyaacgca
      361 vlsliilala kicmknknae veev
//
LOCUS       XP_076546129             926 aa            linear   INV 03-SEP-2025
DEFINITION  kinesin-like protein KIF6 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546129
VERSION     XP_076546129.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690014.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..926
                     /product="kinesin-like protein KIF6 isoform X1"
                     /calculated_mol_wt=106309
     Region          5..384
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Site            order(13..14,100..107,253..254,291..296)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276814"
     Region          369..>437
                     /region_name="PRK13169"
                     /note="DNA replication initiation control protein YabA"
                     /db_xref="CDD:183876"
     CDS             1..926
                     /gene="LOC117600226"
                     /coded_by="XM_076690014.1:355..3135"
                     /db_xref="GeneID:117600226"
ORIGIN      
        1 mvknaikvya rlkpeknrkn imnyqirhrp kekleedflf lvvpsqktke ypdnrpeswn
       61 fsffrifees ttqedvfknv arpvvesald gyngtifayg qtasgktysi tgkqessetl
      121 giiprsllyl feaiqkvefr iiaaqsileq hsrrkggrri ftqpffdslq rpenvysvev
      181 ayleiyneng ydlldrrqqr dnpvtrledl prvavqedei grlhmknltf hcvgnaedaf
      241 elllvgdsnr vtietpmnpe ssrshcifti vvctkkfgad eykrakvhlv dlagservyk
      301 csisgtilte akhinlslhy leqvivclgq estghipyrn slltsilrds lggnclttml
      361 atisvesynl qetvstckfa qrvalirndv kvvlekdvhs enallrlene klrqqlkemt
      421 ketkaeelta eekqrldfni rsflesneqi kvdnnpkkie ycfeslkrif klnsdpdscm
      481 kkleyykdlv iqrdkeisll idlikkerkk gkegccnesr sieeklegnl kviensssls
      541 nfdkiqvnke cngqgdhspk vftetddrms sminnilknt vpskkqkarp kinkslhqrn
      601 lmtnldqnid tsktveqsss nksisndcpk qnslpcniin ltlnnstetd etnqqsfrkk
      661 erkkvksgei easkkkaiei kqnaesssvr iepekievpv letnnvkpyy qrflsfnvpk
      721 nsnnsmtdnl terkfidqtv etknqnkins neqckvskln seensnykpc ldlklnlest
      781 vsqrqeqltd rmrkclnvdh lmssrsigvn srdidvglnq qrnaeslene akeshkqstr
      841 snvcsfdkld snrrktsekr tsissftvdq pekpyvfdeq rkeevnqtsn ydgnkdfegs
      901 lpltgdpeid eeiiafykak rsggty
//
LOCUS       XP_076546130             920 aa            linear   INV 03-SEP-2025
DEFINITION  kinesin-like protein KIF6 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546130
VERSION     XP_076546130.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690015.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..920
                     /product="kinesin-like protein KIF6 isoform X2"
                     /calculated_mol_wt=105617
     Region          5..384
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Site            order(13..14,100..107,253..254,291..296)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276814"
     Region          369..>437
                     /region_name="PRK13169"
                     /note="DNA replication initiation control protein YabA"
                     /db_xref="CDD:183876"
     CDS             1..920
                     /gene="LOC117600226"
                     /coded_by="XM_076690015.1:355..3117"
                     /db_xref="GeneID:117600226"
ORIGIN      
        1 mvknaikvya rlkpeknrkn imnyqirhrp kekleedflf lvvpsqktke ypdnrpeswn
       61 fsffrifees ttqedvfknv arpvvesald gyngtifayg qtasgktysi tgkqessetl
      121 giiprsllyl feaiqkvefr iiaaqsileq hsrrkggrri ftqpffdslq rpenvysvev
      181 ayleiyneng ydlldrrqqr dnpvtrledl prvavqedei grlhmknltf hcvgnaedaf
      241 elllvgdsnr vtietpmnpe ssrshcifti vvctkkfgad eykrakvhlv dlagservyk
      301 csisgtilte akhinlslhy leqvivclgq estghipyrn slltsilrds lggnclttml
      361 atisvesynl qetvstckfa qrvalirndv kvvlekdvhs enallrlene klrqqlkemt
      421 ketkaeelta eekqrldfni rsflesneqi kvdnnpkkie ycfeslkrif klnsdpdscm
      481 kkleyykdlv iqrdkeisll idlikkerkk gkegsieekl egnlkviens sslsnfdkiq
      541 vnkecngqgd hspkvftetd drmssminni lkntvpskkq karpkinksl hqrnlmtnld
      601 qnidtsktve qsssnksisn dcpkqnslpc niinltlnns tetdetnqqs frkkerkkvk
      661 sgeieaskkk aieikqnaes ssvriepeki evpvletnnv kpyyqrflsf nvpknsnnsm
      721 tdnlterkfi dqtvetknqn kinsneqckv sklnseensn ykpcldlkln lestvsqrqe
      781 qltdrmrkcl nvdhlmssrs igvnsrdidv glnqqrnaes leneakeshk qstrsnvcsf
      841 dkldsnrrkt sekrtsissf tvdqpekpyv fdeqrkeevn qtsnydgnkd fegslpltgd
      901 peideeiiaf ykakrsggty
//
LOCUS       XP_076546131             892 aa            linear   INV 03-SEP-2025
DEFINITION  kinesin-like protein KIF6 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546131
VERSION     XP_076546131.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690016.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..892
                     /product="kinesin-like protein KIF6 isoform X3"
                     /calculated_mol_wt=102295
     Region          5..350
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Site            order(13..14,100..107,219..220,257..262)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276814"
     Region          335..>403
                     /region_name="PRK13169"
                     /note="DNA replication initiation control protein YabA"
                     /db_xref="CDD:183876"
     CDS             1..892
                     /gene="LOC117600226"
                     /coded_by="XM_076690016.1:355..3033"
                     /db_xref="GeneID:117600226"
ORIGIN      
        1 mvknaikvya rlkpeknrkn imnyqirhrp kekleedflf lvvpsqktke ypdnrpeswn
       61 fsffrifees ttqedvfknv arpvvesald gyngtifayg qtasgktysi tgkqessetl
      121 giiprsllyl feaiqkrpen vysvevayle iynengydll drrqqrdnpv trledlprva
      181 vqedeigrlh mknltfhcvg naedafelll vgdsnrvtie tpmnpessrs hciftivvct
      241 kkfgadeykr akvhlvdlag servykcsis gtilteakhi nlslhyleqv ivclgqestg
      301 hipyrnsllt silrdslggn clttmlatis vesynlqetv stckfaqrva lirndvkvvl
      361 ekdvhsenal lrleneklrq qlkemtketk aeeltaeekq rldfnirsfl esneqikvdn
      421 npkkieycfe slkrifklns dpdscmkkle yykdlviqrd keisllidli kkerkkgkeg
      481 ccnesrsiee klegnlkvie nssslsnfdk iqvnkecngq gdhspkvfte tddrmssmin
      541 nilkntvpsk kqkarpkink slhqrnlmtn ldqnidtskt veqsssnksi sndcpkqnsl
      601 pcniinltln nstetdetnq qsfrkkerkk vksgeieask kkaieikqna esssvriepe
      661 kievpvletn nvkpyyqrfl sfnvpknsnn smtdnlterk fidqtvetkn qnkinsneqc
      721 kvsklnseen snykpcldlk lnlestvsqr qeqltdrmrk clnvdhlmss rsigvnsrdi
      781 dvglnqqrna esleneakes hkqstrsnvc sfdkldsnrr ktsekrtsis sftvdqpekp
      841 yvfdeqrkee vnqtsnydgn kdfegslplt gdpeideeii afykakrsgg ty
//
LOCUS       XP_076546132             801 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600235 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546132
VERSION     XP_076546132.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690017.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..801
                     /product="uncharacterized protein LOC117600235 isoform X1"
                     /calculated_mol_wt=89772
     CDS             1..801
                     /gene="LOC117600235"
                     /coded_by="XM_076690017.1:4..2409"
                     /db_xref="GeneID:117600235"
ORIGIN      
        1 mfncceeivv fklavfclll llalghcqdn dlpsgyeies sinrtieeve rqvrenpklp
       61 rltrqeivgi lqnitsqdln aykdkeklek arrlyqralm vvlpynaees pgnlndlytk
      121 ppmtqiipdh lkstnniedn evknfetsep svmenlvste nfykpttkdi qstttknkyk
      181 nhrdtysevq dklplkdtlk lestpvrfsf nlenlqknsy ttektttmry pvyrgngekk
      241 sgievvysts vteepftksv pkeiivdldr skatqnvlts sqwrynapps tleptlpsnv
      301 eklpflptvn aepeepivss tkrseilpnd rstaeplimr tegpttlyvt pmstetspkv
      361 kysstyslns agfrtattss tsmrpevmdl lesiglrpdn nsnvediykk nkdmlernpe
      421 lnnivqttis glspvgsdtp sildqntfes sgsvikkgme nltpevrllf qrfglqtsnl
      481 dqpsitttqr iavnsnsytn fkplptssvk dqemkeflak fglgvenrrq ksmesvtkpp
      541 slieavpdsm rnvlenmgli sssqkkmrnp qkvvedmest etsmyhvfkp hevkvgdenq
      601 rskinelldt vklvqqgkad iqnvrkvand liestktlke gpdplkleei irnyskelrn
      661 evkrqeeqeq gttttepttv ssitttesae ttltastsgd stksasdqsa adsstsased
      721 sssstissst sssnlmalee sfggttrepd palppkrktg lyflvdwntf levgedekek
      781 inlrfqpkvg drtrflpvtv p
//
LOCUS       XP_034171254             790 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600235 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171254
VERSION     XP_034171254.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315363.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171254.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..790
                     /product="uncharacterized protein LOC117600235 isoform X2"
                     /calculated_mol_wt=88460
     CDS             1..790
                     /gene="LOC117600235"
                     /coded_by="XM_034315363.2:368..2740"
                     /db_xref="GeneID:117600235"
ORIGIN      
        1 mlavfcllll lalghcqdnd lpsgyeiess inrtieever qvrenpklpr ltrqeivgil
       61 qnitsqdlna ykdkekleka rrlyqralmv vlpynaeesp gnlndlytkp pmtqiipdhl
      121 kstnniedne vknfetseps vmenlvsten fykpttkdiq stttknkykn hrdtysevqd
      181 klplkdtlkl estpvrfsfn lenlqknsyt tektttmryp vyrgngekks gievvystsv
      241 teepftksvp keiivdldrs katqnvltss qwrynappst leptlpsnve klpflptvna
      301 epeepivsst krseilpndr staeplimrt egpttlyvtp mstetspkvk ysstyslnsa
      361 gfrtattsst smrpevmdll esiglrpdnn snvediykkn kdmlernpel nnivqttisg
      421 lspvgsdtps ildqntfess gsvikkgmen ltpevrllfq rfglqtsnld qpsitttqri
      481 avnsnsytnf kplptssvkd qemkeflakf glgvenrrqk smesvtkpps lieavpdsmr
      541 nvlenmglis ssqkkmrnpq kvvedmeste tsmyhvfkph evkvgdenqr skinelldtv
      601 klvqqgkadi qnvrkvandl iestktlkeg pdplkleeii rnyskelrne vkrqeeqeqg
      661 ttttepttvs sitttesaet tltastsgds tksasdqsaa dsstsaseds ssstisssts
      721 ssnlmalees fggttrepdp alppkrktgl yflvdwntfl evgedekeki nlrfqpkvgd
      781 rtrflpvtvp
//
LOCUS       XP_034171205            1856 aa            linear   INV 03-SEP-2025
DEFINITION  female sterile (1) Nasrat [Osmia lignaria lignaria].
ACCESSION   XP_034171205
VERSION     XP_034171205.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315314.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171205.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1856
                     /product="female sterile (1) Nasrat"
                     /calculated_mol_wt=212361
     CDS             1..1856
                     /gene="fs(1)N"
                     /coded_by="XM_034315314.2:161..5731"
                     /db_xref="GeneID:117600218"
ORIGIN      
        1 mspsisrifl flflrlivtr slnveesskt adqlledslk rfprdsvdvs piwmeyefns
       61 gvgnreeliv tdisiqeftn lpkgvnwqff eingtsylac aktsslslyk ldldnvkvin
      121 nvdinirgni ltfkminlns fnvvavlcve ssngtslqwy rlvdevsfqf fwswpvqkqv
      181 qdmefvqqgd enklllldae evlfgqeyss vdvygfsidf pnydftfwly qstlvpkafd
      241 iqvcsiyesi slalrgvndv rlyeykattv esifqglqti kshdlsnfvc fdsgylqfla
      301 isgpeaglfn wiegefrynt dsesnfdise iswvknirln tyrdeallll qlqnktmial
      361 awqglsferi qlpsnildqf dlstitpvpk fgffvgdqfv rfhtelkelk hpvqyttekl
      421 lilqrllndt lyhqekilde tearieksyl knpvtgfwnl ssvnatdani sddvkfhsvt
      481 igsknltree ldfnvnayte elknleekln eidsnimnaa glnikelhfd sdvelfgnin
      541 itgslnvddl svqfindinv mdsfesynen kifssieadn ltirslngip vdklrfsdst
      601 ynfhnvnfsk inrvqidghl ffstingvdw etlmknivwi nqpasipgnt viegtltads
      661 ldldivngll ypddyvlihg dnsvnvtgik sfdklmlprl qevntlngid fqdfiflnrd
      721 nvlkeeitfe nlsvdgqlqi dgevsgfegk gikllnetse aftdvtflnl nvlgnvtfet
      781 lfmnkrslnf gdlllrteen veitgtktfl sdvhmkanvt itsgivnghl lnefvtlnte
      841 qelpnlvril pnvtfgnvtf galkkletlf kensntsnnc ldkiivfksp itvdelsfnk
      901 lnneisyedf qgklnetfqn illenlttdr lfadyitptf ingidfanft kslsspkivn
      961 eyeidrletd hlnatfingm sldeinelnd rlttmfteif sgnvaleslq vtgnieansv
     1021 ngkaledlyn eyqtgkiife edvsirnlti lglmnglnfs efvsdtilkt dtnividghk
     1081 tfdvvncaql eaasingrpv enlfdpvkeq tltgpviidg sitvlnefna tgrinnlsfh
     1141 dlmdrfkslg ndtfelhgdv yfnnnvtler lhingsiqge nfdsflntvv fknednviis
     1201 grkvfensvt fnrgfivhdq lndidlkrfq ekavfidkpf sikskiifid gikvendiav
     1261 ktnfepksim giniedlrin vlylnrptyi tdpitvtdvn flsdiivekf nnidmnllip
     1321 lnteqtipte tlmcqtvnve eidilgming qdlkeiqrtt fmingnqnit ghfnfhghvh
     1381 mqhdfnahli ngidptrmis lkskskiign ftfeepiyln kslrvlgyln didpvrweam
     1441 avttnssqqq ivsgtwtvhg nvyfekgafg sdilngtdit kisntlaekh lemdavlaek
     1501 nenlksvced lrhlkhyaek qiyqfktfdy lqiiefdsni vsvhyfeldh ldymmlsfnt
     1561 chmhtymftg tkfelvakve nfgvverwst frynetlyfl tsgsnscgrs pvnlwkleng
     1621 efthvldlgh nvdsrkined iflmlvnrne qmrssketne ilekslssla ddqvkivled
     1681 qmllsskpei yeynvarfan nslqksvkra evlnfkagal ekkmllyyde eisedrvfic
     1741 kngetrkkiv qtinahrptf ftilnfegsi etllifienr mhlqiyeyrg iqgfvykdsl
     1801 kmdvdklfsf kirkyanlak rhclalihdn rltileaemy gekldmeefs cldkyq
//
LOCUS       XP_034172647             145 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L35A [Osmia lignaria lignaria].
ACCESSION   XP_034172647
VERSION     XP_034172647.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316756.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172647.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..145
                     /product="ribosomal protein L35A"
                     /calculated_mol_wt=16295
     Region          38..138
                     /region_name="Ribosomal_L35Ae"
                     /note="Ribosomal protein L35Ae; pfam01247"
                     /db_xref="CDD:460132"
     CDS             1..145
                     /gene="RpL35A"
                     /coded_by="XM_034316756.2:150..587"
                     /db_xref="GeneID:117600827"
ORIGIN      
        1 madataeska ktktskkpke kpvepllknr pfkrlgrlya kaiftgykrg lrnqhehtal
       61 lkvdgartkc dsdfyvgkrc vyvykaknkt pvpgkkgkkt kvraiwgkvt rphgtsgsvr
      121 akfkknlpak amghririmm ypsri
//
LOCUS       XP_034172655             145 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L35A [Osmia lignaria lignaria].
ACCESSION   XP_034172655
VERSION     XP_034172655.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316764.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172655.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..145
                     /product="ribosomal protein L35A"
                     /calculated_mol_wt=16295
     Region          38..138
                     /region_name="Ribosomal_L35Ae"
                     /note="Ribosomal protein L35Ae; pfam01247"
                     /db_xref="CDD:460132"
     CDS             1..145
                     /gene="RpL35A"
                     /coded_by="XM_034316764.2:157..594"
                     /db_xref="GeneID:117600827"
ORIGIN      
        1 madataeska ktktskkpke kpvepllknr pfkrlgrlya kaiftgykrg lrnqhehtal
       61 lkvdgartkc dsdfyvgkrc vyvykaknkt pvpgkkgkkt kvraiwgkvt rphgtsgsvr
      121 akfkknlpak amghririmm ypsri
//
LOCUS       XP_034183065             474 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605611 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183065
VERSION     XP_034183065.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327174.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="uncharacterized protein LOC117605611 isoform X1"
                     /calculated_mol_wt=52893
     CDS             1..474
                     /gene="LOC117605611"
                     /coded_by="XM_034327174.2:71..1495"
                     /db_xref="GeneID:117605611"
ORIGIN      
        1 mklflilgll cvgihgekki nledierdnl raedvsqpgn trseepkysp kpeigqqqyq
       61 iqnqyngppn sqvayvtpss vpsetyvrag syatkeqayq qqnnilqeqi qspryyneyq
      121 qqsfaakgia pniyetqqlv yqpevnvgnq lqstqqktit akysknvnkd tvyidipvmh
      181 llayypnldv hngknngflv pqfntrehis ipvytsalsq kpivpakpsy qvqyaskyns
      241 vapsafttki tkgtayttpv tskkftnspl tnvptylspd qsyaqgrqfl ytqayiapsq
      301 sqyvpqfvys qpttvymhat pvysdiyart ptyvqdnalq agskykpssd qldtgvpvad
      361 elagqvlvqq tsqsvsqnyv kdlgepstdl vppqaaaqnf qsapllpvss qeeesvpdqn
      421 hvglseprsl ldsyvpskli aaqdsaryqe rpikleggfl pskenflykk rktd
//
LOCUS       XP_034183153             474 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605611 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183153
VERSION     XP_034183153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327262.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="uncharacterized protein LOC117605611 isoform X2"
                     /calculated_mol_wt=52893
     CDS             1..474
                     /gene="LOC117605611"
                     /coded_by="XM_034327262.2:45..1469"
                     /db_xref="GeneID:117605611"
ORIGIN      
        1 mklflilgll cvgihgekki nledierdnl raedvsqpgn trseepkysp kpeigqqqyq
       61 iqnqyngppn sqvayvtpss vpsetyvrag syatkeqayq qqnnilqeqi qspryyneyq
      121 qqsfaakgia pniyetqqlv yqpevnvgnq lqstqqktit akysknvnkd tvyidipvmh
      181 llayypnldv hngknngflv pqfntrehis ipvytsalsq kpivpakpsy qvqyaskyns
      241 vapsafttki tkgtayttpv tskkftnspl tnvptylspd qsyaqgrqfl ytqayiapsq
      301 sqyvpqfvys qpttvymhat pvysdiyart ptyvqdnalq agskykpssd qldtgvpvad
      361 elagqvlvqq tsqsvsqnyv kdlgepstdl vppqaaaqnf qsapllpvss qeeesvpdqn
      421 hvglseprsl ldsyvpskli aaqdsaryqe rpikleggfl pskenflykk rktd
//
LOCUS       XP_034179554             202 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603999 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179554
VERSION     XP_034179554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323663.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="uncharacterized protein LOC117603999 isoform X1"
                     /calculated_mol_wt=22909
     CDS             1..202
                     /gene="LOC117603999"
                     /coded_by="XM_034323663.2:13..621"
                     /db_xref="GeneID:117603999"
ORIGIN      
        1 mvklrvlvvl alfvifnvnl vepmvhesls klfgslsgvi hvripellnq lcnesqrsnt
       61 vlrdacygcf yrttnqpvgy rlllsmsnca niylnstdya hcqeylnnat stanaranpt
      121 tiyctflecv rqvnkdnlea lrvkpraked srktnrkvsf lyfklykqia rsicvirerd
      181 sesntvrpga epvdpreain kp
//
LOCUS       XP_076546133             243 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603999 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546133
VERSION     XP_076546133.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690018.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="uncharacterized protein LOC117603999 isoform X2"
                     /calculated_mol_wt=27322
     CDS             1..243
                     /gene="LOC117603999"
                     /coded_by="XM_076690018.1:19..750"
                     /db_xref="GeneID:117603999"
ORIGIN      
        1 mvklrvlvvl alfvifnvnl vepmvhesls klfgslsgvi hvripellnq lcnesqrsnt
       61 vlrdacygcf yrttnqpvgy rlllsmsnca niylnstdya hcqeylnnat stanaranpt
      121 tiyctflecv rqvnkdnllr ecvgealrmf pnfnttdvkl aqlyvnttac vlaktrcaht
      181 npitgeyqkd dlanklhipa inamlvntdy dinilqlpfh ygsidvcaky rnyeqaswps
      241 amc
//
LOCUS       XP_034178821            1289 aa            linear   INV 03-SEP-2025
DEFINITION  DOMON-like domain-containing protein nahoda isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034178821
VERSION     XP_034178821.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322930.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178821.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1289
                     /product="DOMON-like domain-containing protein nahoda
                     isoform X1"
                     /calculated_mol_wt=142953
     Region          <45..155
                     /region_name="LPMO_10"
                     /note="Lytic polysaccharide mono-oxygenase,
                     cellulose-degrading; pfam03067"
                     /db_xref="CDD:460793"
     Region          194..231
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <630..730
                     /region_name="DOMON_DOH"
                     /note="DOMON-like domain of copper-dependent
                     monooxygenases and related proteins; cd09631"
                     /db_xref="CDD:187689"
     Region          803..918
                     /region_name="DOMON"
                     /note="DOMON domain; pfam03351"
                     /db_xref="CDD:460893"
     CDS             1..1289
                     /gene="nahoda"
                     /coded_by="XM_034322930.2:1178..5047"
                     /db_xref="GeneID:117603590"
ORIGIN      
        1 mlsktcsaim sglmllvltv itfrevqghv altfprarky dldfldnart pgpcgmprgd
       61 vkttflsgsn fnvtwhlayp hrggfrlqil daldrplvdl tpvtknsefv eddataqsya
      121 vqlpqnftct dctirllrea eewgssyrfw scadidiidr ksyredcsgh gryllgrcrc
      181 drlyhgtrce fkeeclddsd cgiqgtcidn ggttaptkhc ycnigwfgps cakkspikti
      241 dvdldsytmk kfsdnftfyw rilkeskele gvmvvngtsw vgvgwrpsdl tpmcrsfpni
      301 qerpkdeplp qpepksepep ksepkaepka epepepepea epesgterss akrrsakahd
      361 iallaatprs dtdvtvqtsv tyrvstrqgr kkrspesaaa stngepvaep stvnstntks
      421 epepksepep ksepepssep epesepepss epepksepep ssepepepks qsesisktks
      481 nsktesrfka elisaaepks epepksepep isepksepep ssepksepep ssepkskpep
      541 ssepepesep epepksepep ssepepksep epksepepss epepksepep ksepepksep
      601 tsepepisds nssatkaalp gsghkftpkh dfnpmdctdi iigsargnsh rigdyytrdr
      661 stprkdeywg grdtltaamg yerdgvttil frrklaanep tdheivddnm qviwakgqep
      721 gnyvhhppsg iektkvkvkd fykpdelkyh ghgsqrgtvt lnffdeknrq eamsgnntlq
      781 isnctgewry prhclpengt ceysiqwsyk grkdqlsfvi ttthtntwtg vgfsddekms
      841 qtdavlgwvd dksgraflmd twisgynapl ldpsqdiynt ggskvdgvtt lkfsrkritk
      901 dnrdlsftdd hclymmypvk ggvfnavskk irkhdavpiv ssericirsc gleeleemtt
      961 papprlhynv evklinlgdg fttpapgtpd fevisnrisd sfgpvldklp gyyqirlnel
     1021 kkddeqngvi armdlildkn evkgrslkpl dtnaaaekal kealvngkvg alsvdpqylv
     1081 iraprvndfg gggdpeegss stfgsdlfld atklyilvgc vaallalall qasctlyrss
     1141 rrratkdrli asnawkdyas gantnfafea feteekappp pvsslprhre mtgnglgtdt
     1201 vegptrmvgp ratyslprap gtrhtapiqp gyytqdrrdh yqrsknnahn asgvstqpnq
     1261 pdfyfmpsqr kysgevvrvy vdygsqtpk
//
LOCUS       XP_034178831            1289 aa            linear   INV 03-SEP-2025
DEFINITION  DOMON-like domain-containing protein nahoda isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034178831
VERSION     XP_034178831.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322940.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178831.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1289
                     /product="DOMON-like domain-containing protein nahoda
                     isoform X1"
                     /calculated_mol_wt=142953
     Region          <45..155
                     /region_name="LPMO_10"
                     /note="Lytic polysaccharide mono-oxygenase,
                     cellulose-degrading; pfam03067"
                     /db_xref="CDD:460793"
     Region          194..231
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <630..730
                     /region_name="DOMON_DOH"
                     /note="DOMON-like domain of copper-dependent
                     monooxygenases and related proteins; cd09631"
                     /db_xref="CDD:187689"
     Region          803..918
                     /region_name="DOMON"
                     /note="DOMON domain; pfam03351"
                     /db_xref="CDD:460893"
     CDS             1..1289
                     /gene="nahoda"
                     /coded_by="XM_034322940.2:544..4413"
                     /db_xref="GeneID:117603590"
ORIGIN      
        1 mlsktcsaim sglmllvltv itfrevqghv altfprarky dldfldnart pgpcgmprgd
       61 vkttflsgsn fnvtwhlayp hrggfrlqil daldrplvdl tpvtknsefv eddataqsya
      121 vqlpqnftct dctirllrea eewgssyrfw scadidiidr ksyredcsgh gryllgrcrc
      181 drlyhgtrce fkeeclddsd cgiqgtcidn ggttaptkhc ycnigwfgps cakkspikti
      241 dvdldsytmk kfsdnftfyw rilkeskele gvmvvngtsw vgvgwrpsdl tpmcrsfpni
      301 qerpkdeplp qpepksepep ksepkaepka epepepepea epesgterss akrrsakahd
      361 iallaatprs dtdvtvqtsv tyrvstrqgr kkrspesaaa stngepvaep stvnstntks
      421 epepksepep ksepepssep epesepepss epepksepep ssepepepks qsesisktks
      481 nsktesrfka elisaaepks epepksepep isepksepep ssepksepep ssepkskpep
      541 ssepepesep epepksepep ssepepksep epksepepss epepksepep ksepepksep
      601 tsepepisds nssatkaalp gsghkftpkh dfnpmdctdi iigsargnsh rigdyytrdr
      661 stprkdeywg grdtltaamg yerdgvttil frrklaanep tdheivddnm qviwakgqep
      721 gnyvhhppsg iektkvkvkd fykpdelkyh ghgsqrgtvt lnffdeknrq eamsgnntlq
      781 isnctgewry prhclpengt ceysiqwsyk grkdqlsfvi ttthtntwtg vgfsddekms
      841 qtdavlgwvd dksgraflmd twisgynapl ldpsqdiynt ggskvdgvtt lkfsrkritk
      901 dnrdlsftdd hclymmypvk ggvfnavskk irkhdavpiv ssericirsc gleeleemtt
      961 papprlhynv evklinlgdg fttpapgtpd fevisnrisd sfgpvldklp gyyqirlnel
     1021 kkddeqngvi armdlildkn evkgrslkpl dtnaaaekal kealvngkvg alsvdpqylv
     1081 iraprvndfg gggdpeegss stfgsdlfld atklyilvgc vaallalall qasctlyrss
     1141 rrratkdrli asnawkdyas gantnfafea feteekappp pvsslprhre mtgnglgtdt
     1201 vegptrmvgp ratyslprap gtrhtapiqp gyytqdrrdh yqrsknnahn asgvstqpnq
     1261 pdfyfmpsqr kysgevvrvy vdygsqtpk
//
LOCUS       XP_034178837            1206 aa            linear   INV 03-SEP-2025
DEFINITION  DOMON-like domain-containing protein nahoda isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034178837
VERSION     XP_034178837.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322946.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178837.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1206
                     /product="DOMON-like domain-containing protein nahoda
                     isoform X2"
                     /calculated_mol_wt=134272
     Region          <45..155
                     /region_name="LPMO_10"
                     /note="Lytic polysaccharide mono-oxygenase,
                     cellulose-degrading; pfam03067"
                     /db_xref="CDD:460793"
     Region          194..231
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <630..730
                     /region_name="DOMON_DOH"
                     /note="DOMON-like domain of copper-dependent
                     monooxygenases and related proteins; cd09631"
                     /db_xref="CDD:187689"
     Region          803..918
                     /region_name="DOMON"
                     /note="DOMON domain; pfam03351"
                     /db_xref="CDD:460893"
     CDS             1..1206
                     /gene="nahoda"
                     /coded_by="XM_034322946.2:1185..4805"
                     /db_xref="GeneID:117603590"
ORIGIN      
        1 mlsktcsaim sglmllvltv itfrevqghv altfprarky dldfldnart pgpcgmprgd
       61 vkttflsgsn fnvtwhlayp hrggfrlqil daldrplvdl tpvtknsefv eddataqsya
      121 vqlpqnftct dctirllrea eewgssyrfw scadidiidr ksyredcsgh gryllgrcrc
      181 drlyhgtrce fkeeclddsd cgiqgtcidn ggttaptkhc ycnigwfgps cakkspikti
      241 dvdldsytmk kfsdnftfyw rilkeskele gvmvvngtsw vgvgwrpsdl tpmcrsfpni
      301 qerpkdeplp qpepksepep ksepkaepka epepepepea epesgterss akrrsakahd
      361 iallaatprs dtdvtvqtsv tyrvstrqgr kkrspesaaa stngepvaep stvnstntks
      421 epepksepep ksepepssep epesepepss epepksepep ssepepepks qsesisktks
      481 nsktesrfka elisaaepks epepksepep isepksepep ssepksepep ssepkskpep
      541 ssepepesep epepksepep ssepepksep epksepepss epepksepep ksepepksep
      601 tsepepisds nssatkaalp gsghkftpkh dfnpmdctdi iigsargnsh rigdyytrdr
      661 stprkdeywg grdtltaamg yerdgvttil frrklaanep tdheivddnm qviwakgqep
      721 gnyvhhppsg iektkvkvkd fykpdelkyh ghgsqrgtvt lnffdeknrq eamsgnntlq
      781 isnctgewry prhclpengt ceysiqwsyk grkdqlsfvi ttthtntwtg vgfsddekms
      841 qtdavlgwvd dksgraflmd twisgynapl ldpsqdiynt ggskvdgvtt lkfsrkritk
      901 dnrdlsftdd hclymmypvk ggvfnavskk irkhdavpiv ssericirsc gleeleemtt
      961 papprlhynv evklinlgdg fttpapgtpd fevisnrisd sfgpvldklp gyyqirlnel
     1021 kkddeqngvi armdlildkn evkgrslkpl dtnaaaekal kealvngkvg alsvdpqylv
     1081 iraprvndfg gggdpeegss stfgsdlfld atklyilvgc vaallalall qasctlyrss
     1141 rrratkrvys lplascdapn pihrtnatry hrqngdhhhh hnnhhhhlrh hhqhapydmh
     1201 rpnpsw
//
LOCUS       XP_034178838            1076 aa            linear   INV 03-SEP-2025
DEFINITION  DOMON-like domain-containing protein nahoda isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034178838
VERSION     XP_034178838.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322947.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178838.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1076
                     /product="DOMON-like domain-containing protein nahoda
                     isoform X3"
                     /calculated_mol_wt=120019
     Region          <45..155
                     /region_name="LPMO_10"
                     /note="Lytic polysaccharide mono-oxygenase,
                     cellulose-degrading; pfam03067"
                     /db_xref="CDD:460793"
     Region          194..231
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          <417..517
                     /region_name="DOMON_DOH"
                     /note="DOMON-like domain of copper-dependent
                     monooxygenases and related proteins; cd09631"
                     /db_xref="CDD:187689"
     Region          590..705
                     /region_name="DOMON"
                     /note="DOMON domain; pfam03351"
                     /db_xref="CDD:460893"
     CDS             1..1076
                     /gene="nahoda"
                     /coded_by="XM_034322947.2:1176..4406"
                     /db_xref="GeneID:117603590"
ORIGIN      
        1 mlsktcsaim sglmllvltv itfrevqghv altfprarky dldfldnart pgpcgmprgd
       61 vkttflsgsn fnvtwhlayp hrggfrlqil daldrplvdl tpvtknsefv eddataqsya
      121 vqlpqnftct dctirllrea eewgssyrfw scadidiidr ksyredcsgh gryllgrcrc
      181 drlyhgtrce fkeeclddsd cgiqgtcidn ggttaptkhc ycnigwfgps cakkspikti
      241 dvdldsytmk kfsdnftfyw rilkeskele gvmvvngtsw vgvgwrpsdl tpmcrsfpni
      301 qerpkdeplp qpepksepep ksepkaepka epepepepea epesgterss akrrsakahd
      361 iallaatprs dtdvtvqtsv tyrvstrqgr kkrspesaaa stnaalpgsg hkftpkhdfn
      421 pmdctdiiig sargnshrig dyytrdrstp rkdeywggrd tltaamgyer dgvttilfrr
      481 klaaneptdh eivddnmqvi wakgqepgny vhhppsgiek tkvkvkdfyk pdelkyhghg
      541 sqrgtvtlnf fdeknrqeam sgnntlqisn ctgewryprh clpengtcey siqwsykgrk
      601 dqlsfvittt htntwtgvgf sddekmsqtd avlgwvddks graflmdtwi sgynaplldp
      661 sqdiyntggs kvdgvttlkf srkritkdnr dlsftddhcl ymmypvkggv fnavskkirk
      721 hdavpivsse ricirscgle eleemttpap prlhynvevk linlgdgftt papgtpdfev
      781 isnrisdsfg pvldklpgyy qirlnelkkd deqngviarm dlildknevk grslkpldtn
      841 aaaekalkea lvngkvgals vdpqylvira prvndfgggg dpeegssstf gsdlfldatk
      901 lyilvgcvaa llalallqas ctlyrssrrr atkdrliasn awkdyasgan tnfafeafet
      961 eekappppvs slprhremtg nglgtdtveg ptrmvgprat yslprapgtr htapiqpgyy
     1021 tqdrrdhyqr sknnahnasg vstqpnqpdf yfmpsqrkys gevvrvyvdy gsqtpk
//
LOCUS       XP_034171935            1183 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034171935
VERSION     XP_034171935.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316044.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171935.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1183
                     /product="zinc finger protein MEP-1 isoform X1"
                     /calculated_mol_wt=130753
     Region          <100..387
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          995..1021
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(1002..1004,1006..1007,1011,1020,1038,1041..1045,
                     1047..1048)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          1033..1054
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1183
                     /gene="MEP-1"
                     /coded_by="XM_034316044.2:447..3998"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 mvrvylvpss srtsigitmf igfwflllyr qkrlnictkk pfkcsivvgd qcimigfdql
       61 nsagpmegtl eeppvkdssq aspdkvlkdv ipngvngnyn dreefddani naskfvskei
      121 nsavkgnrea ngkeddedeh kmdvdieerd lesedseqng iaesrsnrge msesendskt
      181 skssegvset dnfsevskte dkckntscea mevdeqsnns dvecldesvt eihsdnddvf
      241 tmknsavkdi qnlssssdiq indsneiads sldtsdvkic eengscgspd ieeitdsakn
      301 ghnsssertn kdptkqrkpr kqidlgsitp rrssrnikrt syiekeieed vdddgsdiee
      361 ikpedplagi dskdkenskl kspiknsktt ivvndtkrlv eiaagsksvk ggkkeptlvi
      421 idtnsilsgr ggvpvssskp hhtasstssf svvpmgmttq tmypnmrtti tpvpmtsksa
      481 qlspktssmt tvtptvtppi lptltddmfv veapsfivpy vyekpplkpl kefvtklekc
      541 leekekeern nrimeenkee cdddssdisq knkdkdeese negnskskdg edkgdnsldl
      601 tesgfsnisd kqdirqddrn kvltyfdlpl gkffmqigvn lvqeyvqtdl lrtqkrkqgk
      661 gstsaetqla insliknlef skennepfhl elkkcefcsf ktestlvmqh hletphmrny
      721 vykcnfcpve vrsphdilfh meaehntrgr lergpafhqc pncpfednqk gkltrhilac
      781 tkkfrpeknl epaadweppa kiprlnrtrp vgptnpsala mamsgkgpqp llpkllpapi
      841 tgrgrgrapm qprypdlktl rpggttmrqd nvagmmyrpt ssgllvptsy qfgsnqifqv
      901 vggsgtvmsa vsgvssssgg ssgqptpial vpnvidslsr lsssqnttsp knptakllsq
      961 psisitplpr ttsqtsvpgs gtssksggkt tfviceicdg yikdleqlrn hmqwihkvki
     1021 hpkmiynrpp lncqkcqfrf ftdqglerhl lgshglvtss mqeaankgkd agrcpacgrv
     1081 yqwkllnhva rdhgmtlkpa hlsykctvct atfgmykqfe nhvysahsvv akrvmdkknt
     1141 psspssrsnd sllkplkind eitiipqpak pttrsgtsqg rgk
//
LOCUS       XP_034171942            1137 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034171942
VERSION     XP_034171942.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316051.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171942.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1137
                     /product="zinc finger protein MEP-1 isoform X2"
                     /calculated_mol_wt=126466
     Region          <100..387
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          949..975
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(956..958,960..961,965,974,992,995..999,1001..1002)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          987..1008
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1137
                     /gene="MEP-1"
                     /coded_by="XM_034316051.2:445..3858"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 mvrvylvpss srtsigitmf igfwflllyr qkrlnictkk pfkcsivvgd qcimigfdql
       61 nsagpmegtl eeppvkdssq aspdkvlkdv ipngvngnyn dreefddani naskfvskei
      121 nsavkgnrea ngkeddedeh kmdvdieerd lesedseqng iaesrsnrge msesendskt
      181 skssegvset dnfsevskte dkckntscea mevdeqsnns dvecldesvt eihsdnddvf
      241 tmknsavkdi qnlssssdiq indsneiads sldtsdvkic eengscgspd ieeitdsakn
      301 ghnsssertn kdptkqrkpr kqidlgsitp rrssrnikrt syiekeieed vdddgsdiee
      361 ikpedplagi dskdkenskl kspiknsktt ivvndtkrlv eiaagsksvk ggkkeptlvi
      421 idtnsilsgr ggvpvssskp hhtasstssf svvpmgmttq tmypnmrtti tpvpmtsksa
      481 qlspktssmt tvtptvtppi lptltddmfv veapsfivpy vyekpplkpl kefvtklekc
      541 leekekeern nrimeenkee cdddssdisq knkdkdeese negnskskdg edkgdnsldl
      601 tesgfsnisd kqdirqddrn kvltyfdlpl gkffmqigvn lvqeyvqtdl lrtqkrkqgk
      661 gstsaetqla insliknlef skennepfhl elkkcefcsf ktestlvmqh hletphmrny
      721 vykcnfcpve vrsphdilfh meaehntrgr lergpafhqc pncpfednqk gkltrhilac
      781 tkkfrpeknl epaadweppa kiprlnrtrp vgptnpsala mamsgkgpqp llpkllpapi
      841 tgrgrgrapm qprypdlktl rpggttmrqd nvagmmyrpt ssgllvptsy qfgsnqifqn
      901 ttspknptak llsqpsisit plprttsqts vpgsgtssks ggkttfvice icdgyikdle
      961 qlrnhmqwih kvkihpkmiy nrpplncqkc qfrfftdqgl erhllgshgl vtssmqeaan
     1021 kgkdagrcpa cgrvyqwkll nhvardhgmt lkpahlsykc tvctatfgmy kqfenhvysa
     1081 hsvvakrvmd kkntpsspss rsndsllkpl kindeitiip qpakpttrsg tsqgrgk
//
LOCUS       XP_034171950            1133 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034171950
VERSION     XP_034171950.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316059.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171950.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1133
                     /product="zinc finger protein MEP-1 isoform X3"
                     /calculated_mol_wt=124962
     Region          <50..337
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          945..971
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(952..954,956..957,961,970,988,991..995,997..998)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          983..1004
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1133
                     /gene="MEP-1"
                     /coded_by="XM_034316059.2:112..3513"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 mkgessmyql nsagpmegtl eeppvkdssq aspdkvlkdv ipngvngnyn dreefddani
       61 naskfvskei nsavkgnrea ngkeddedeh kmdvdieerd lesedseqng iaesrsnrge
      121 msesendskt skssegvset dnfsevskte dkckntscea mevdeqsnns dvecldesvt
      181 eihsdnddvf tmknsavkdi qnlssssdiq indsneiads sldtsdvkic eengscgspd
      241 ieeitdsakn ghnsssertn kdptkqrkpr kqidlgsitp rrssrnikrt syiekeieed
      301 vdddgsdiee ikpedplagi dskdkenskl kspiknsktt ivvndtkrlv eiaagsksvk
      361 ggkkeptlvi idtnsilsgr ggvpvssskp hhtasstssf svvpmgmttq tmypnmrtti
      421 tpvpmtsksa qlspktssmt tvtptvtppi lptltddmfv veapsfivpy vyekpplkpl
      481 kefvtklekc leekekeern nrimeenkee cdddssdisq knkdkdeese negnskskdg
      541 edkgdnsldl tesgfsnisd kqdirqddrn kvltyfdlpl gkffmqigvn lvqeyvqtdl
      601 lrtqkrkqgk gstsaetqla insliknlef skennepfhl elkkcefcsf ktestlvmqh
      661 hletphmrny vykcnfcpve vrsphdilfh meaehntrgr lergpafhqc pncpfednqk
      721 gkltrhilac tkkfrpeknl epaadweppa kiprlnrtrp vgptnpsala mamsgkgpqp
      781 llpkllpapi tgrgrgrapm qprypdlktl rpggttmrqd nvagmmyrpt ssgllvptsy
      841 qfgsnqifqv vggsgtvmsa vsgvssssgg ssgqptpial vpnvidslsr lsssqnttsp
      901 knptakllsq psisitplpr ttsqtsvpgs gtssksggkt tfviceicdg yikdleqlrn
      961 hmqwihkvki hpkmiynrpp lncqkcqfrf ftdqglerhl lgshglvtss mqeaankgkd
     1021 agrcpacgrv yqwkllnhva rdhgmtlkpa hlsykctvct atfgmykqfe nhvysahsvv
     1081 akrvmdkknt psspssrsnd sllkplkind eitiipqpak pttrsgtsqg rgk
//
LOCUS       XP_034171969            1118 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034171969
VERSION     XP_034171969.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316078.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171969.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="zinc finger protein MEP-1 isoform X4"
                     /calculated_mol_wt=123381
     Region          <35..322
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          930..956
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(937..939,941..942,946,955,973,976..980,982..983)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          968..989
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1118
                     /gene="MEP-1"
                     /coded_by="XM_034316078.2:286..3642"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 megtleeppv kdssqaspdk vlkdvipngv ngnyndreef ddaninaskf vskeinsavk
       61 gnreangked dedehkmdvd ieerdlesed seqngiaesr snrgemsese ndsktsksse
      121 gvsetdnfse vsktedkckn tsceamevde qsnnsdvecl desvteihsd nddvftmkns
      181 avkdiqnlss ssdiqindsn eiadssldts dvkiceengs cgspdieeit dsaknghnss
      241 sertnkdptk qrkprkqidl gsitprrssr nikrtsyiek eieedvdddg sdieeikped
      301 plagidskdk ensklkspik nskttivvnd tkrlveiaag sksvkggkke ptlviidtns
      361 ilsgrggvpv ssskphhtas stssfsvvpm gmttqtmypn mrttitpvpm tsksaqlspk
      421 tssmttvtpt vtppilptlt ddmfvveaps fivpyvyekp plkplkefvt klekcleeke
      481 keernnrime enkeecddds sdisqknkdk deesenegns kskdgedkgd nsldltesgf
      541 snisdkqdir qddrnkvlty fdlplgkffm qigvnlvqey vqtdllrtqk rkqgkgstsa
      601 etqlainsli knlefskenn epfhlelkkc efcsfktest lvmqhhletp hmrnyvykcn
      661 fcpvevrsph dilfhmeaeh ntrgrlergp afhqcpncpf ednqkgkltr hilactkkfr
      721 peknlepaad weppakiprl nrtrpvgptn psalamamsg kgpqpllpkl lpapitgrgr
      781 grapmqpryp dlktlrpggt tmrqdnvagm myrptssgll vptsyqfgsn qifqvvggsg
      841 tvmsavsgvs sssggssgqp tpialvpnvi dslsrlsssq nttspknpta kllsqpsisi
      901 tplprttsqt svpgsgtssk sggkttfvic eicdgyikdl eqlrnhmqwi hkvkihpkmi
      961 ynrpplncqk cqfrfftdqg lerhllgshg lvtssmqeaa nkgkdagrcp acgrvyqwkl
     1021 lnhvardhgm tlkpahlsyk ctvctatfgm ykqfenhvys ahsvvakrvm dkkntpssps
     1081 srsndsllkp lkindeitii pqpakpttrs gtsqgrgk
//
LOCUS       XP_076546135            1118 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546135
VERSION     XP_076546135.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690020.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="zinc finger protein MEP-1 isoform X4"
                     /calculated_mol_wt=123381
     Region          <35..322
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          930..956
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(937..939,941..942,946,955,973,976..980,982..983)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          968..989
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1118
                     /gene="MEP-1"
                     /coded_by="XM_076690020.1:188..3544"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 megtleeppv kdssqaspdk vlkdvipngv ngnyndreef ddaninaskf vskeinsavk
       61 gnreangked dedehkmdvd ieerdlesed seqngiaesr snrgemsese ndsktsksse
      121 gvsetdnfse vsktedkckn tsceamevde qsnnsdvecl desvteihsd nddvftmkns
      181 avkdiqnlss ssdiqindsn eiadssldts dvkiceengs cgspdieeit dsaknghnss
      241 sertnkdptk qrkprkqidl gsitprrssr nikrtsyiek eieedvdddg sdieeikped
      301 plagidskdk ensklkspik nskttivvnd tkrlveiaag sksvkggkke ptlviidtns
      361 ilsgrggvpv ssskphhtas stssfsvvpm gmttqtmypn mrttitpvpm tsksaqlspk
      421 tssmttvtpt vtppilptlt ddmfvveaps fivpyvyekp plkplkefvt klekcleeke
      481 keernnrime enkeecddds sdisqknkdk deesenegns kskdgedkgd nsldltesgf
      541 snisdkqdir qddrnkvlty fdlplgkffm qigvnlvqey vqtdllrtqk rkqgkgstsa
      601 etqlainsli knlefskenn epfhlelkkc efcsfktest lvmqhhletp hmrnyvykcn
      661 fcpvevrsph dilfhmeaeh ntrgrlergp afhqcpncpf ednqkgkltr hilactkkfr
      721 peknlepaad weppakiprl nrtrpvgptn psalamamsg kgpqpllpkl lpapitgrgr
      781 grapmqpryp dlktlrpggt tmrqdnvagm myrptssgll vptsyqfgsn qifqvvggsg
      841 tvmsavsgvs sssggssgqp tpialvpnvi dslsrlsssq nttspknpta kllsqpsisi
      901 tplprttsqt svpgsgtssk sggkttfvic eicdgyikdl eqlrnhmqwi hkvkihpkmi
      961 ynrpplncqk cqfrfftdqg lerhllgshg lvtssmqeaa nkgkdagrcp acgrvyqwkl
     1021 lnhvardhgm tlkpahlsyk ctvctatfgm ykqfenhvys ahsvvakrvm dkkntpssps
     1081 srsndsllkp lkindeitii pqpakpttrs gtsqgrgk
//
LOCUS       XP_034171960            1118 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034171960
VERSION     XP_034171960.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316069.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171960.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="zinc finger protein MEP-1 isoform X4"
                     /calculated_mol_wt=123381
     Region          <35..322
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          930..956
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(937..939,941..942,946,955,973,976..980,982..983)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          968..989
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1118
                     /gene="MEP-1"
                     /coded_by="XM_034316069.2:405..3761"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 megtleeppv kdssqaspdk vlkdvipngv ngnyndreef ddaninaskf vskeinsavk
       61 gnreangked dedehkmdvd ieerdlesed seqngiaesr snrgemsese ndsktsksse
      121 gvsetdnfse vsktedkckn tsceamevde qsnnsdvecl desvteihsd nddvftmkns
      181 avkdiqnlss ssdiqindsn eiadssldts dvkiceengs cgspdieeit dsaknghnss
      241 sertnkdptk qrkprkqidl gsitprrssr nikrtsyiek eieedvdddg sdieeikped
      301 plagidskdk ensklkspik nskttivvnd tkrlveiaag sksvkggkke ptlviidtns
      361 ilsgrggvpv ssskphhtas stssfsvvpm gmttqtmypn mrttitpvpm tsksaqlspk
      421 tssmttvtpt vtppilptlt ddmfvveaps fivpyvyekp plkplkefvt klekcleeke
      481 keernnrime enkeecddds sdisqknkdk deesenegns kskdgedkgd nsldltesgf
      541 snisdkqdir qddrnkvlty fdlplgkffm qigvnlvqey vqtdllrtqk rkqgkgstsa
      601 etqlainsli knlefskenn epfhlelkkc efcsfktest lvmqhhletp hmrnyvykcn
      661 fcpvevrsph dilfhmeaeh ntrgrlergp afhqcpncpf ednqkgkltr hilactkkfr
      721 peknlepaad weppakiprl nrtrpvgptn psalamamsg kgpqpllpkl lpapitgrgr
      781 grapmqpryp dlktlrpggt tmrqdnvagm myrptssgll vptsyqfgsn qifqvvggsg
      841 tvmsavsgvs sssggssgqp tpialvpnvi dslsrlsssq nttspknpta kllsqpsisi
      901 tplprttsqt svpgsgtssk sggkttfvic eicdgyikdl eqlrnhmqwi hkvkihpkmi
      961 ynrpplncqk cqfrfftdqg lerhllgshg lvtssmqeaa nkgkdagrcp acgrvyqwkl
     1021 lnhvardhgm tlkpahlsyk ctvctatfgm ykqfenhvys ahsvvakrvm dkkntpssps
     1081 srsndsllkp lkindeitii pqpakpttrs gtsqgrgk
//
LOCUS       XP_034171979            1118 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034171979
VERSION     XP_034171979.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316088.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171979.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="zinc finger protein MEP-1 isoform X4"
                     /calculated_mol_wt=123381
     Region          <35..322
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          930..956
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(937..939,941..942,946,955,973,976..980,982..983)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          968..989
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1118
                     /gene="MEP-1"
                     /coded_by="XM_034316088.2:271..3627"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 megtleeppv kdssqaspdk vlkdvipngv ngnyndreef ddaninaskf vskeinsavk
       61 gnreangked dedehkmdvd ieerdlesed seqngiaesr snrgemsese ndsktsksse
      121 gvsetdnfse vsktedkckn tsceamevde qsnnsdvecl desvteihsd nddvftmkns
      181 avkdiqnlss ssdiqindsn eiadssldts dvkiceengs cgspdieeit dsaknghnss
      241 sertnkdptk qrkprkqidl gsitprrssr nikrtsyiek eieedvdddg sdieeikped
      301 plagidskdk ensklkspik nskttivvnd tkrlveiaag sksvkggkke ptlviidtns
      361 ilsgrggvpv ssskphhtas stssfsvvpm gmttqtmypn mrttitpvpm tsksaqlspk
      421 tssmttvtpt vtppilptlt ddmfvveaps fivpyvyekp plkplkefvt klekcleeke
      481 keernnrime enkeecddds sdisqknkdk deesenegns kskdgedkgd nsldltesgf
      541 snisdkqdir qddrnkvlty fdlplgkffm qigvnlvqey vqtdllrtqk rkqgkgstsa
      601 etqlainsli knlefskenn epfhlelkkc efcsfktest lvmqhhletp hmrnyvykcn
      661 fcpvevrsph dilfhmeaeh ntrgrlergp afhqcpncpf ednqkgkltr hilactkkfr
      721 peknlepaad weppakiprl nrtrpvgptn psalamamsg kgpqpllpkl lpapitgrgr
      781 grapmqpryp dlktlrpggt tmrqdnvagm myrptssgll vptsyqfgsn qifqvvggsg
      841 tvmsavsgvs sssggssgqp tpialvpnvi dslsrlsssq nttspknpta kllsqpsisi
      901 tplprttsqt svpgsgtssk sggkttfvic eicdgyikdl eqlrnhmqwi hkvkihpkmi
      961 ynrpplncqk cqfrfftdqg lerhllgshg lvtssmqeaa nkgkdagrcp acgrvyqwkl
     1021 lnhvardhgm tlkpahlsyk ctvctatfgm ykqfenhvys ahsvvakrvm dkkntpssps
     1081 srsndsllkp lkindeitii pqpakpttrs gtsqgrgk
//
LOCUS       XP_034171987            1118 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034171987
VERSION     XP_034171987.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316096.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034171987.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="zinc finger protein MEP-1 isoform X4"
                     /calculated_mol_wt=123381
     Region          <35..322
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          930..956
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(937..939,941..942,946,955,973,976..980,982..983)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          968..989
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1118
                     /gene="MEP-1"
                     /coded_by="XM_034316096.2:106..3462"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 megtleeppv kdssqaspdk vlkdvipngv ngnyndreef ddaninaskf vskeinsavk
       61 gnreangked dedehkmdvd ieerdlesed seqngiaesr snrgemsese ndsktsksse
      121 gvsetdnfse vsktedkckn tsceamevde qsnnsdvecl desvteihsd nddvftmkns
      181 avkdiqnlss ssdiqindsn eiadssldts dvkiceengs cgspdieeit dsaknghnss
      241 sertnkdptk qrkprkqidl gsitprrssr nikrtsyiek eieedvdddg sdieeikped
      301 plagidskdk ensklkspik nskttivvnd tkrlveiaag sksvkggkke ptlviidtns
      361 ilsgrggvpv ssskphhtas stssfsvvpm gmttqtmypn mrttitpvpm tsksaqlspk
      421 tssmttvtpt vtppilptlt ddmfvveaps fivpyvyekp plkplkefvt klekcleeke
      481 keernnrime enkeecddds sdisqknkdk deesenegns kskdgedkgd nsldltesgf
      541 snisdkqdir qddrnkvlty fdlplgkffm qigvnlvqey vqtdllrtqk rkqgkgstsa
      601 etqlainsli knlefskenn epfhlelkkc efcsfktest lvmqhhletp hmrnyvykcn
      661 fcpvevrsph dilfhmeaeh ntrgrlergp afhqcpncpf ednqkgkltr hilactkkfr
      721 peknlepaad weppakiprl nrtrpvgptn psalamamsg kgpqpllpkl lpapitgrgr
      781 grapmqpryp dlktlrpggt tmrqdnvagm myrptssgll vptsyqfgsn qifqvvggsg
      841 tvmsavsgvs sssggssgqp tpialvpnvi dslsrlsssq nttspknpta kllsqpsisi
      901 tplprttsqt svpgsgtssk sggkttfvic eicdgyikdl eqlrnhmqwi hkvkihpkmi
      961 ynrpplncqk cqfrfftdqg lerhllgshg lvtssmqeaa nkgkdagrcp acgrvyqwkl
     1021 lnhvardhgm tlkpahlsyk ctvctatfgm ykqfenhvys ahsvvakrvm dkkntpssps
     1081 srsndsllkp lkindeitii pqpakpttrs gtsqgrgk
//
LOCUS       XP_076546137            1118 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein MEP-1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546137
VERSION     XP_076546137.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690022.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="zinc finger protein MEP-1 isoform X4"
                     /calculated_mol_wt=123381
     Region          <35..322
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          930..956
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(937..939,941..942,946,955,973,976..980,982..983)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          968..989
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..1118
                     /gene="MEP-1"
                     /coded_by="XM_076690022.1:230..3586"
                     /db_xref="GeneID:117600499"
ORIGIN      
        1 megtleeppv kdssqaspdk vlkdvipngv ngnyndreef ddaninaskf vskeinsavk
       61 gnreangked dedehkmdvd ieerdlesed seqngiaesr snrgemsese ndsktsksse
      121 gvsetdnfse vsktedkckn tsceamevde qsnnsdvecl desvteihsd nddvftmkns
      181 avkdiqnlss ssdiqindsn eiadssldts dvkiceengs cgspdieeit dsaknghnss
      241 sertnkdptk qrkprkqidl gsitprrssr nikrtsyiek eieedvdddg sdieeikped
      301 plagidskdk ensklkspik nskttivvnd tkrlveiaag sksvkggkke ptlviidtns
      361 ilsgrggvpv ssskphhtas stssfsvvpm gmttqtmypn mrttitpvpm tsksaqlspk
      421 tssmttvtpt vtppilptlt ddmfvveaps fivpyvyekp plkplkefvt klekcleeke
      481 keernnrime enkeecddds sdisqknkdk deesenegns kskdgedkgd nsldltesgf
      541 snisdkqdir qddrnkvlty fdlplgkffm qigvnlvqey vqtdllrtqk rkqgkgstsa
      601 etqlainsli knlefskenn epfhlelkkc efcsfktest lvmqhhletp hmrnyvykcn
      661 fcpvevrsph dilfhmeaeh ntrgrlergp afhqcpncpf ednqkgkltr hilactkkfr
      721 peknlepaad weppakiprl nrtrpvgptn psalamamsg kgpqpllpkl lpapitgrgr
      781 grapmqpryp dlktlrpggt tmrqdnvagm myrptssgll vptsyqfgsn qifqvvggsg
      841 tvmsavsgvs sssggssgqp tpialvpnvi dslsrlsssq nttspknpta kllsqpsisi
      901 tplprttsqt svpgsgtssk sggkttfvic eicdgyikdl eqlrnhmqwi hkvkihpkmi
      961 ynrpplncqk cqfrfftdqg lerhllgshg lvtssmqeaa nkgkdagrcp acgrvyqwkl
     1021 lnhvardhgm tlkpahlsyk ctvctatfgm ykqfenhvys ahsvvakrvm dkkntpssps
     1081 srsndsllkp lkindeitii pqpakpttrs gtsqgrgk
//
LOCUS       XP_034175849            1137 aa            linear   INV 03-SEP-2025
DEFINITION  SPT16 homolog, facilitates chromatin remodeling subunit dre4
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175849
VERSION     XP_034175849.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319958.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1137
                     /product="SPT16 homolog, facilitates chromatin remodeling
                     subunit dre4 isoform X1"
                     /calculated_mol_wt=128994
     Region          1..1006
                     /region_name="COG5406"
                     /note="Nucleosome binding factor SPN, SPT16 subunit
                     [Transcription, Replication, recombination and repair,
                     Chromatin structure and dynamics]"
                     /db_xref="CDD:227693"
     CDS             1..1137
                     /gene="dre4"
                     /coded_by="XM_034319958.2:143..3556"
                     /db_xref="GeneID:117602156"
ORIGIN      
        1 manvsvdket ffrrmkrlys awkdgevgtd dsfskmdclv savgtdediv yskstalqtw
       61 llsyeltdti milaeesicf laskkkiefl rklenqkaee tgvppvkllv rdrndedkan
      121 faklietikq skkgktlgvf skenypgafm dawraalkse sfdtvdvsaa aayvmcpked
      181 aeiltikkac lvsvdvftky lkdqimeiid sdkkvkhskl aegvdaaitn kkyvtgvdvt
      241 qvdmcypaii qsggnyslkf svvsdkntlh fgvivcslga ryksycsniv rtllvnptkq
      301 iednynfllq leeeilkkli agvkisevye tgvkyvkdek pemldhltkn fgfamgiefr
      361 esslligpkt havikkgmvf nvnvglanls npdatekegk vyalfigdtv mvnegqpavn
      421 ltpskkklkn vgifvkdeee eeeegsgken epkpeilgrg krtavieskl rtehsseekr
      481 kqhqkelaqq lnevakarla qqsggkeqek irkstisyks lshmprepev kelklyvdkk
      541 yetvilpifg ipvpfhisti knisqsvegd ytylrinffh pgatmgrneg gsypqpdatf
      601 vkevtyrstn tkepgeisap ssnlntafrl ikevqkkfkn reaeereked lvkqdtlils
      661 qnkgnpklkd lyirpnivsk rmtggleahv ngfrytsvrg dkvdilynni knaffqpcdg
      721 emiillhfhl khaimfgkkk hvdvqfytev geittdlgkh qhmhdrddla aeqserelrh
      781 klktafksfc ekvesmtkqe iefdtpfrel gfpgapfrst vllqptsgcl vnltewppfv
      841 itledvelvh fervqfhlkn fdmifvfkdy hrkvailnai pmnmldhvke wlnscdiryt
      901 egvqslnwtk imktitddpe gffdnggwtf ldpesdaene dledeeeeed dayeptdlds
      961 eeesdddsey seasedsdse eeelgssees gkdwsdlere aaeedkerge dgfrddynss
     1021 kkkkssrkhs pspskdrhns khksssssks kssssskdkk ssssdkhrsd rsrsgrshkk
     1081 vspstsskhs pkksdrhdkh dkrdkhksss hsssnskkrs reesserndr gskkskk
//
LOCUS       XP_034175937            1136 aa            linear   INV 03-SEP-2025
DEFINITION  SPT16 homolog, facilitates chromatin remodeling subunit dre4
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034175937
VERSION     XP_034175937.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320046.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1136
                     /product="SPT16 homolog, facilitates chromatin remodeling
                     subunit dre4 isoform X2"
                     /calculated_mol_wt=128865
     Region          1..1005
                     /region_name="COG5406"
                     /note="Nucleosome binding factor SPN, SPT16 subunit
                     [Transcription, Replication, recombination and repair,
                     Chromatin structure and dynamics]"
                     /db_xref="CDD:227693"
     CDS             1..1136
                     /gene="dre4"
                     /coded_by="XM_034320046.2:279..3689"
                     /db_xref="GeneID:117602156"
ORIGIN      
        1 manvsvdket ffrrmkrlys awkdgevgtd dsfskmdclv savgtdediv yskstalqtw
       61 llsyeltdti milaeesicf laskkkiefl rklenqkaee tgvppvkllv rdrndedkan
      121 faklietikq skkgktlgvf skenypgafm dawraalkse sfdtvdvsaa aayvmcpked
      181 aeiltikkac lvsvdvftky lkdqimeiid sdkkvkhskl aegvdaaitn kkyvtgvdvt
      241 qvdmcypaii qsggnyslkf svvsdkntlh fgvivcslga ryksycsniv rtllvnptkq
      301 iednynfllq leeeilkkli agvkisevye tgvkyvkdek pemldhltkn fgfamgiefr
      361 esslligpkt havikkgmvf nvnvglanls npdatekegk vyalfigdtv mvnegqpavn
      421 ltpskkklkn vgifvkdeee eeeegsgken epkpeilgrg krtavieskl rtehsseekr
      481 kqhqkelaqq lnevakarla qqsggkeqek irkstisyks lshmprepev kelklyvdkk
      541 yetvilpifg ipvpfhisti knisqsvegd ytylrinffh pgatmgrneg gsypqpdatf
      601 vkevtyrstn tkepgeisap ssnlntafrl ikevqkkfkn reaeereked lvkqdtlils
      661 qnkgnpklkd lyirpnivsk rmtggleahv ngfrytsvrg dkvdilynni knaffqpcdg
      721 emiillhfhl khaimfgkkk hvdvqfytev geittdlgkh qhmhdrddla aeqserelrh
      781 klktafksfc ekvesmtkqe iefdtpfrel gfpgapfrst vllqptsgcl vnltewppfv
      841 itledvelvh fervqfhlkn fdmifvfkdy hrkvailnai pmnmldhvke wlnscdiryt
      901 egvqslnwtk imktitddpe gffdnggwtf ldpesdaene dledeeeeed dayeptdlds
      961 eeesdddsey seasedsdse eelgsseesg kdwsdlerea aeedkerged gfrddynssk
     1021 kkkssrkhsp spskdrhnsk hksssssksk ssssskdkks sssdkhrsdr srsgrshkkv
     1081 spstsskhsp kksdrhdkhd krdkhksssh sssnskkrsr eesserndrg skkskk
//
LOCUS       XP_034176022            1115 aa            linear   INV 03-SEP-2025
DEFINITION  SPT16 homolog, facilitates chromatin remodeling subunit dre4
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034176022
VERSION     XP_034176022.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1115
                     /product="SPT16 homolog, facilitates chromatin remodeling
                     subunit dre4 isoform X3"
                     /calculated_mol_wt=126544
     Region          1..1006
                     /region_name="COG5406"
                     /note="Nucleosome binding factor SPN, SPT16 subunit
                     [Transcription, Replication, recombination and repair,
                     Chromatin structure and dynamics]"
                     /db_xref="CDD:227693"
     CDS             1..1115
                     /gene="dre4"
                     /coded_by="XM_034320131.2:280..3627"
                     /db_xref="GeneID:117602156"
ORIGIN      
        1 manvsvdket ffrrmkrlys awkdgevgtd dsfskmdclv savgtdediv yskstalqtw
       61 llsyeltdti milaeesicf laskkkiefl rklenqkaee tgvppvkllv rdrndedkan
      121 faklietikq skkgktlgvf skenypgafm dawraalkse sfdtvdvsaa aayvmcpked
      181 aeiltikkac lvsvdvftky lkdqimeiid sdkkvkhskl aegvdaaitn kkyvtgvdvt
      241 qvdmcypaii qsggnyslkf svvsdkntlh fgvivcslga ryksycsniv rtllvnptkq
      301 iednynfllq leeeilkkli agvkisevye tgvkyvkdek pemldhltkn fgfamgiefr
      361 esslligpkt havikkgmvf nvnvglanls npdatekegk vyalfigdtv mvnegqpavn
      421 ltpskkklkn vgifvkdeee eeeegsgken epkpeilgrg krtavieskl rtehsseekr
      481 kqhqkelaqq lnevakarla qqsggkeqek irkstisyks lshmprepev kelklyvdkk
      541 yetvilpifg ipvpfhisti knisqsvegd ytylrinffh pgatmgrneg gsypqpdatf
      601 vkevtyrstn tkepgeisap ssnlntafrl ikevqkkfkn reaeereked lvkqdtlils
      661 qnkgnpklkd lyirpnivsk rmtggleahv ngfrytsvrg dkvdilynni knaffqpcdg
      721 emiillhfhl khaimfgkkk hvdvqfytev geittdlgkh qhmhdrddla aeqserelrh
      781 klktafksfc ekvesmtkqe iefdtpfrel gfpgapfrst vllqptsgcl vnltewppfv
      841 itledvelvh fervqfhlkn fdmifvfkdy hrkvailnai pmnmldhvke wlnscdiryt
      901 egvqslnwtk imktitddpe gffdnggwtf ldpesdaene dledeeeeed dayeptdlds
      961 eeesdddsey seasedsdse eeelgssees gkdwsdlere aaeedkerge dgfrddynss
     1021 kkkkssrkhs pspskdrhns khksssssks kssssskdkk ssssdkhspk ksdrhdkhdk
     1081 rdkhkssshs ssnskkrsre esserndrgs kkskk
//
LOCUS       XP_034188082             988 aa            linear   INV 03-SEP-2025
DEFINITION  poly-(ADP-ribose) polymerase [Osmia lignaria lignaria].
ACCESSION   XP_034188082
VERSION     XP_034188082.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188082.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..988
                     /product="poly-(ADP-ribose) polymerase"
                     /calculated_mol_wt=112462
     Region          1..987
                     /region_name="PLN03123"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215590"
     CDS             1..988
                     /gene="Parp1"
                     /coded_by="XM_034332191.2:102..3068"
                     /db_xref="GeneID:117607905"
ORIGIN      
        1 mnadqpysve yaksarascq nckssiakds lrlavvvqsp mhdgliprwy hpdcffmkqr
       61 pkitadianf dnirwedqki iekkieestn lplptsgrgk krgnaaakna gplgdflvqy
      121 aksnkstcka ceerivqgei riskkdfese hgrryggidr whhlecfakv reslefyesg
      181 dvlpgikdfs kedqakvkna lpkiksddip ikkvknetqd aeeeeemkeq nqeifkikdi
      241 lspikkldli amlekneqqi pegvpaiidr ladaiyfgal kpctkcngql aytsgigykc
      301 tgnltewtkc eyvtqdpkrk kfvipanlke pypglkslkc kvrkrlikla vpststsvkk
      361 dvvdsgvkie akpkplkhmq fviigrtern kdslkkdiml lggdvtskih ehvaaiisnq
      421 qeiekmnkki eeakkfniqv itedfieeak eyimpaitli qkktisswgg nlsdrinvva
      481 eksaakskgk sifeksasgk vklqikegst vdpdsglqdc ahvyqkgkdk ytvtlvhtdi
      541 qskknsfykl qilkhdkqnk ywlfrswgri gttiggtkld slhledskaq feflyedktg
      601 nrwsqrehfv kvphkmypvd vdygdevttk lldsdiesnl ekpvqdlirl ifdeghmkkv
      661 maefeidvdk mplgkltkkq iqkaysvlte lqellknkpv dsvllldasn rfynliphnf
      721 gvsgpkvldt ieeihvkcem ldallemeia ysllhtktda tknpldvhye qlhtdikild
      781 keseeykvid qyvknthgkt htqyelvien vfvvkrqgee srykpfkklh nrkllwhgsr
      841 ttnfagilsq glriappeap vtgymfgkgi yfadmvtksa nycctnsgdp tgllllseva
      901 lgnmyeryra dyieklpqgk hstwghgqtq pdpevvhkme dgvevpygpa vpaklpkksd
      961 llynefivyd vaqvkvrylv rmyfkykv
//
LOCUS       XP_034176149             929 aa            linear   INV 03-SEP-2025
DEFINITION  protein tyrosine phosphatase Meg isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176149
VERSION     XP_034176149.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320258.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..929
                     /product="protein tyrosine phosphatase Meg isoform X1"
                     /calculated_mol_wt=104780
     Region          68..263
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          257..351
                     /region_name="FERM_C_PTPN4_PTPN3_like"
                     /note="FERM domain C-lobe of Protein tyrosine phosphatase
                     non-receptor proteins 3 and 4 (PTPN4 and PTPN3); cd13189"
                     /db_xref="CDD:270010"
     Site            order(267,284,286,292)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270010"
     Site            order(296,301..304,339,343,346..347)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Site            339..350
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Region          363..403
                     /region_name="FA"
                     /note="FERM adjacent (FA); pfam08736"
                     /db_xref="CDD:462582"
     Region          515..604
                     /region_name="PDZ_PTPN3-4-like"
                     /note="PDZ domain of tyrosine-protein phosphatase
                     non-receptor type 3 (PTPN3), tyrosine-protein phosphatase
                     non-receptor type 4 (PTNP4), and related domains; cd06706"
                     /db_xref="CDD:467190"
     Site            order(529..535,547,550,581,585,588..589)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467190"
     Region          707..918
                     /region_name="PTPc-N3_4"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 21 and type 14; cd14541"
                     /db_xref="CDD:350389"
     CDS             1..929
                     /gene="Ptpmeg"
                     /coded_by="XM_034320258.2:161..2950"
                     /db_xref="GeneID:117602339"
ORIGIN      
        1 mnlfsmtsse cgqhsreavs grwltrlqif emiesvsrra lsgssgsyhv rgaelarnrr
       61 lkslsatvvf lddtqhtfql dkrakgqall dlvfqhlelv ekdyfglqya engattctys
      121 pdvmrwldps kpvkkqirsk ggqfyfrvkf yvsdpsklqe eytryqfylq irrdilqgkl
      181 qlppstacli asytvqselg dyhpeehgpg ylsrlqlipg qteemekkis elhklhkgql
      241 padaefnfld hakrldmygv elhkardstn keiqlgvtsi glvvfqngik invfswskiv
      301 kisfkrkqff iqlrreqsen ydtllgfnmq tyrssknlwk acvehhtffr lhspktrprr
      361 fpltlssrft ysgrtefqtv edgkhrarve rtfirspskr lvhgvtsasi ieekgklsip
      421 pgrpprpydn kvqslgarep rqawgegnps ddeggflslr eeitgshtqg nafspilgsr
      481 vlsyvdddtt aerniydlpd yseptsspap qivedglvti sltpdeqgrf gfnvkggldl
      541 dmpilvsrva pntpadrcyp klnegdqvvy ingidvsgll hehvvnlirq srdsgsgelt
      601 ltvrpnalyn alagtdetse eeppyryvpd aphaaigsda laqsmlllad glasgaliaq
      661 yeqlyrknpe ltsleskkpe nqsknryrdi spydvtrvil mgsasgdyin anyvnmeipg
      721 sgiinryiat qgplsstvad fwqmvleags tlvvmlttlv drgrakchqy wpalnetltl
      781 rnltltstae nvedtfifre filrdintge erdithmqyc swpdhgvpsd wrqfttfter
      841 vraartgmve pavvhcsagi grtgvlvlme talclieanq pvypldivrs mrdqrammiq
      901 nasqyrfvce avhkaysegi akplpefsr
//
LOCUS       XP_034176237             927 aa            linear   INV 03-SEP-2025
DEFINITION  protein tyrosine phosphatase Meg isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176237
VERSION     XP_034176237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320346.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..927
                     /product="protein tyrosine phosphatase Meg isoform X2"
                     /calculated_mol_wt=104595
     Region          68..261
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          255..349
                     /region_name="FERM_C_PTPN4_PTPN3_like"
                     /note="FERM domain C-lobe of Protein tyrosine phosphatase
                     non-receptor proteins 3 and 4 (PTPN4 and PTPN3); cd13189"
                     /db_xref="CDD:270010"
     Site            order(265,282,284,290)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270010"
     Site            order(294,299..302,337,341,344..345)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Site            337..348
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Region          361..401
                     /region_name="FA"
                     /note="FERM adjacent (FA); pfam08736"
                     /db_xref="CDD:462582"
     Region          513..602
                     /region_name="PDZ_PTPN3-4-like"
                     /note="PDZ domain of tyrosine-protein phosphatase
                     non-receptor type 3 (PTPN3), tyrosine-protein phosphatase
                     non-receptor type 4 (PTNP4), and related domains; cd06706"
                     /db_xref="CDD:467190"
     Site            order(527..533,545,548,579,583,586..587)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467190"
     Region          705..916
                     /region_name="PTPc-N3_4"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 21 and type 14; cd14541"
                     /db_xref="CDD:350389"
     CDS             1..927
                     /gene="Ptpmeg"
                     /coded_by="XM_034320346.2:161..2944"
                     /db_xref="GeneID:117602339"
ORIGIN      
        1 mnlfsmtsse cgqhsreavs grwltrlqif emiesvsrra lsgssgsyhv rgaelarnrr
       61 lkslsatvvf lddtqhtfql dkrakgqall dlvfqhlelv ekdyfglqya engattctys
      121 pdvmrwldps kpvkkqirsg qfyfrvkfyv sdpsklqeey tryqfylqir rdilqgklql
      181 ppstaclias ytvqselgdy hpeehgpgyl srlqlipgqt eemekkisel hklhkgqlpa
      241 daefnfldha krldmygvel hkardstnke iqlgvtsigl vvfqngikin vfswskivki
      301 sfkrkqffiq lrreqsenyd tllgfnmqty rssknlwkac vehhtffrlh spktrprrfp
      361 ltlssrftys grtefqtved gkhrarvert firspskrlv hgvtsasiie ekgklsippg
      421 rpprpydnkv qslgareprq awgegnpsdd eggflslree itgshtqgna fspilgsrvl
      481 syvdddttae rniydlpdys eptsspapqi vedglvtisl tpdeqgrfgf nvkggldldm
      541 pilvsrvapn tpadrcypkl negdqvvyin gidvsgllhe hvvnlirqsr dsgsgeltlt
      601 vrpnalynal agtdetseee ppyryvpdap haaigsdala qsmllladgl asgaliaqye
      661 qlyrknpelt sleskkpenq sknryrdisp ydvtrvilmg sasgdyinan yvnmeipgsg
      721 iinryiatqg plsstvadfw qmvleagstl vvmlttlvdr grakchqywp alnetltlrn
      781 ltltstaenv edtfifrefi lrdintgeer dithmqycsw pdhgvpsdwr qfttftervr
      841 aartgmvepa vvhcsagigr tgvlvlmeta lclieanqpv ypldivrsmr dqrammiqna
      901 sqyrfvceav hkaysegiak plpefsr
//
LOCUS       XP_034176328             927 aa            linear   INV 03-SEP-2025
DEFINITION  protein tyrosine phosphatase Meg isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176328
VERSION     XP_034176328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..927
                     /product="protein tyrosine phosphatase Meg isoform X3"
                     /calculated_mol_wt=104550
     Region          68..261
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          255..349
                     /region_name="FERM_C_PTPN4_PTPN3_like"
                     /note="FERM domain C-lobe of Protein tyrosine phosphatase
                     non-receptor proteins 3 and 4 (PTPN4 and PTPN3); cd13189"
                     /db_xref="CDD:270010"
     Site            order(265,282,284,290)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270010"
     Site            order(294,299..302,337,341,344..345)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Site            337..348
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Region          361..401
                     /region_name="FA"
                     /note="FERM adjacent (FA); pfam08736"
                     /db_xref="CDD:462582"
     Region          513..602
                     /region_name="PDZ_PTPN3-4-like"
                     /note="PDZ domain of tyrosine-protein phosphatase
                     non-receptor type 3 (PTPN3), tyrosine-protein phosphatase
                     non-receptor type 4 (PTNP4), and related domains; cd06706"
                     /db_xref="CDD:467190"
     Site            order(527..533,545,548,579,583,586..587)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467190"
     Region          705..916
                     /region_name="PTPc-N3_4"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 21 and type 14; cd14541"
                     /db_xref="CDD:350389"
     CDS             1..927
                     /gene="Ptpmeg"
                     /coded_by="XM_034320437.2:161..2944"
                     /db_xref="GeneID:117602339"
ORIGIN      
        1 mnlfsmtsse cgqhsreavs grwltrlqif emiesvsrra lsgssgsyhv rgaelarnrr
       61 lkslsatvvf lddtqhtfql dkrakgqall dlvfqhlelv ekdyfglqya engattctys
      121 pdrwldpskp vkkqirskgg qfyfrvkfyv sdpsklqeey tryqfylqir rdilqgklql
      181 ppstaclias ytvqselgdy hpeehgpgyl srlqlipgqt eemekkisel hklhkgqlpa
      241 daefnfldha krldmygvel hkardstnke iqlgvtsigl vvfqngikin vfswskivki
      301 sfkrkqffiq lrreqsenyd tllgfnmqty rssknlwkac vehhtffrlh spktrprrfp
      361 ltlssrftys grtefqtved gkhrarvert firspskrlv hgvtsasiie ekgklsippg
      421 rpprpydnkv qslgareprq awgegnpsdd eggflslree itgshtqgna fspilgsrvl
      481 syvdddttae rniydlpdys eptsspapqi vedglvtisl tpdeqgrfgf nvkggldldm
      541 pilvsrvapn tpadrcypkl negdqvvyin gidvsgllhe hvvnlirqsr dsgsgeltlt
      601 vrpnalynal agtdetseee ppyryvpdap haaigsdala qsmllladgl asgaliaqye
      661 qlyrknpelt sleskkpenq sknryrdisp ydvtrvilmg sasgdyinan yvnmeipgsg
      721 iinryiatqg plsstvadfw qmvleagstl vvmlttlvdr grakchqywp alnetltlrn
      781 ltltstaenv edtfifrefi lrdintgeer dithmqycsw pdhgvpsdwr qfttftervr
      841 aartgmvepa vvhcsagigr tgvlvlmeta lclieanqpv ypldivrsmr dqrammiqna
      901 sqyrfvceav hkaysegiak plpefsr
//
LOCUS       XP_034176417             926 aa            linear   INV 03-SEP-2025
DEFINITION  protein tyrosine phosphatase Meg isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176417
VERSION     XP_034176417.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320526.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..926
                     /product="protein tyrosine phosphatase Meg isoform X4"
                     /calculated_mol_wt=104440
     Region          68..263
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          257..351
                     /region_name="FERM_C_PTPN4_PTPN3_like"
                     /note="FERM domain C-lobe of Protein tyrosine phosphatase
                     non-receptor proteins 3 and 4 (PTPN4 and PTPN3); cd13189"
                     /db_xref="CDD:270010"
     Site            order(267,284,286,292)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270010"
     Site            order(296,301..304,339,343,346..347)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Site            339..350
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Region          363..400
                     /region_name="FA"
                     /note="FERM adjacent (FA); pfam08736"
                     /db_xref="CDD:462582"
     Region          512..601
                     /region_name="PDZ_PTPN3-4-like"
                     /note="PDZ domain of tyrosine-protein phosphatase
                     non-receptor type 3 (PTPN3), tyrosine-protein phosphatase
                     non-receptor type 4 (PTNP4), and related domains; cd06706"
                     /db_xref="CDD:467190"
     Site            order(526..532,544,547,578,582,585..586)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467190"
     Region          704..915
                     /region_name="PTPc-N3_4"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 21 and type 14; cd14541"
                     /db_xref="CDD:350389"
     CDS             1..926
                     /gene="Ptpmeg"
                     /coded_by="XM_034320526.2:161..2941"
                     /db_xref="GeneID:117602339"
ORIGIN      
        1 mnlfsmtsse cgqhsreavs grwltrlqif emiesvsrra lsgssgsyhv rgaelarnrr
       61 lkslsatvvf lddtqhtfql dkrakgqall dlvfqhlelv ekdyfglqya engattctys
      121 pdvmrwldps kpvkkqirsk ggqfyfrvkf yvsdpsklqe eytryqfylq irrdilqgkl
      181 qlppstacli asytvqselg dyhpeehgpg ylsrlqlipg qteemekkis elhklhkgql
      241 padaefnfld hakrldmygv elhkardstn keiqlgvtsi glvvfqngik invfswskiv
      301 kisfkrkqff iqlrreqsen ydtllgfnmq tyrssknlwk acvehhtffr lhspktrprr
      361 fpltlssrft ysgrtefqtv edgkhrarve rtfiskrlvh gvtsasiiee kgklsippgr
      421 pprpydnkvq slgareprqa wgegnpsdde ggflslreei tgshtqgnaf spilgsrvls
      481 yvdddttaer niydlpdyse ptsspapqiv edglvtislt pdeqgrfgfn vkggldldmp
      541 ilvsrvapnt padrcypkln egdqvvying idvsgllheh vvnlirqsrd sgsgeltltv
      601 rpnalynala gtdetseeep pyryvpdaph aaigsdalaq smllladgla sgaliaqyeq
      661 lyrknpelts leskkpenqs knryrdispy dvtrvilmgs asgdyinany vnmeipgsgi
      721 inryiatqgp lsstvadfwq mvleagstlv vmlttlvdrg rakchqywpa lnetltlrnl
      781 tltstaenve dtfifrefil rdintgeerd ithmqycswp dhgvpsdwrq fttftervra
      841 artgmvepav vhcsagigrt gvlvlmetal clieanqpvy pldivrsmrd qrammiqnas
      901 qyrfvceavh kaysegiakp lpefsr
//
LOCUS       XP_034176509             925 aa            linear   INV 03-SEP-2025
DEFINITION  protein tyrosine phosphatase Meg isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176509
VERSION     XP_034176509.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320618.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="protein tyrosine phosphatase Meg isoform X5"
                     /calculated_mol_wt=104364
     Region          68..259
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          253..347
                     /region_name="FERM_C_PTPN4_PTPN3_like"
                     /note="FERM domain C-lobe of Protein tyrosine phosphatase
                     non-receptor proteins 3 and 4 (PTPN4 and PTPN3); cd13189"
                     /db_xref="CDD:270010"
     Site            order(263,280,282,288)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270010"
     Site            order(292,297..300,335,339,342..343)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Site            335..346
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Region          359..399
                     /region_name="FA"
                     /note="FERM adjacent (FA); pfam08736"
                     /db_xref="CDD:462582"
     Region          511..600
                     /region_name="PDZ_PTPN3-4-like"
                     /note="PDZ domain of tyrosine-protein phosphatase
                     non-receptor type 3 (PTPN3), tyrosine-protein phosphatase
                     non-receptor type 4 (PTNP4), and related domains; cd06706"
                     /db_xref="CDD:467190"
     Site            order(525..531,543,546,577,581,584..585)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467190"
     Region          703..914
                     /region_name="PTPc-N3_4"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 21 and type 14; cd14541"
                     /db_xref="CDD:350389"
     CDS             1..925
                     /gene="Ptpmeg"
                     /coded_by="XM_034320618.2:161..2938"
                     /db_xref="GeneID:117602339"
ORIGIN      
        1 mnlfsmtsse cgqhsreavs grwltrlqif emiesvsrra lsgssgsyhv rgaelarnrr
       61 lkslsatvvf lddtqhtfql dkrakgqall dlvfqhlelv ekdyfglqya engattctys
      121 pdrwldpskp vkkqirsgqf yfrvkfyvsd psklqeeytr yqfylqirrd ilqgklqlpp
      181 stacliasyt vqselgdyhp eehgpgylsr lqlipgqtee mekkiselhk lhkgqlpada
      241 efnfldhakr ldmygvelhk ardstnkeiq lgvtsiglvv fqngikinvf swskivkisf
      301 krkqffiqlr reqsenydtl lgfnmqtyrs sknlwkacve hhtffrlhsp ktrprrfplt
      361 lssrftysgr tefqtvedgk hrarvertfi rspskrlvhg vtsasiieek gklsippgrp
      421 prpydnkvqs lgareprqaw gegnpsddeg gflslreeit gshtqgnafs pilgsrvlsy
      481 vdddttaern iydlpdysep tsspapqive dglvtisltp deqgrfgfnv kggldldmpi
      541 lvsrvapntp adrcypklne gdqvvyingi dvsgllhehv vnlirqsrds gsgeltltvr
      601 pnalynalag tdetseeepp yryvpdapha aigsdalaqs mllladglas galiaqyeql
      661 yrknpeltsl eskkpenqsk nryrdispyd vtrvilmgsa sgdyinanyv nmeipgsgii
      721 nryiatqgpl sstvadfwqm vleagstlvv mlttlvdrgr akchqywpal netltlrnlt
      781 ltstaenved tfifrefilr dintgeerdi thmqycswpd hgvpsdwrqf ttftervraa
      841 rtgmvepavv hcsagigrtg vlvlmetalc lieanqpvyp ldivrsmrdq rammiqnasq
      901 yrfvceavhk aysegiakpl pefsr
//
LOCUS       XP_034176603             898 aa            linear   INV 03-SEP-2025
DEFINITION  protein tyrosine phosphatase Meg isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176603
VERSION     XP_034176603.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320712.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..898
                     /product="protein tyrosine phosphatase Meg isoform X6"
                     /calculated_mol_wt=101196
     Region          37..232
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          226..320
                     /region_name="FERM_C_PTPN4_PTPN3_like"
                     /note="FERM domain C-lobe of Protein tyrosine phosphatase
                     non-receptor proteins 3 and 4 (PTPN4 and PTPN3); cd13189"
                     /db_xref="CDD:270010"
     Site            order(236,253,255,261)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270010"
     Site            order(265,270..273,308,312,315..316)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Site            308..319
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270010"
     Region          332..372
                     /region_name="FA"
                     /note="FERM adjacent (FA); pfam08736"
                     /db_xref="CDD:462582"
     Region          484..573
                     /region_name="PDZ_PTPN3-4-like"
                     /note="PDZ domain of tyrosine-protein phosphatase
                     non-receptor type 3 (PTPN3), tyrosine-protein phosphatase
                     non-receptor type 4 (PTNP4), and related domains; cd06706"
                     /db_xref="CDD:467190"
     Site            order(498..504,516,519,550,554,557..558)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467190"
     Region          676..887
                     /region_name="PTPc-N3_4"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 21 and type 14; cd14541"
                     /db_xref="CDD:350389"
     CDS             1..898
                     /gene="Ptpmeg"
                     /coded_by="XM_034320712.2:659..3355"
                     /db_xref="GeneID:117602339"
ORIGIN      
        1 miesvsrral sgssgsyhvr gaelarnrrl kslsatvvfl ddtqhtfqld krakgqalld
       61 lvfqhlelve kdyfglqyae ngattctysp dvmrwldpsk pvkkqirskg gqfyfrvkfy
      121 vsdpsklqee ytryqfylqi rrdilqgklq lppstaclia sytvqselgd yhpeehgpgy
      181 lsrlqlipgq teemekkise lhklhkgqlp adaefnfldh akrldmygve lhkardstnk
      241 eiqlgvtsig lvvfqngiki nvfswskivk isfkrkqffi qlrreqseny dtllgfnmqt
      301 yrssknlwka cvehhtffrl hspktrprrf pltlssrfty sgrtefqtve dgkhrarver
      361 tfirspskrl vhgvtsasii eekgklsipp grpprpydnk vqslgarepr qawgegnpsd
      421 deggflslre eitgshtqgn afspilgsrv lsyvdddtta erniydlpdy septsspapq
      481 ivedglvtis ltpdeqgrfg fnvkggldld mpilvsrvap ntpadrcypk lnegdqvvyi
      541 ngidvsgllh ehvvnlirqs rdsgsgeltl tvrpnalyna lagtdetsee eppyryvpda
      601 phaaigsdal aqsmllladg lasgaliaqy eqlyrknpel tsleskkpen qsknryrdis
      661 pydvtrvilm gsasgdyina nyvnmeipgs giinryiatq gplsstvadf wqmvleagst
      721 lvvmlttlvd rgrakchqyw palnetltlr nltltstaen vedtfifref ilrdintgee
      781 rdithmqycs wpdhgvpsdw rqfttfterv raartgmvep avvhcsagig rtgvlvlmet
      841 alclieanqp vypldivrsm rdqrammiqn asqyrfvcea vhkaysegia kplpefsr
//
LOCUS       XP_034178846             883 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603625 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178846
VERSION     XP_034178846.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322955.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178846.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..883
                     /product="uncharacterized protein LOC117603625 isoform X1"
                     /calculated_mol_wt=95583
     Region          240..336
                     /region_name="PET_Prickle"
                     /note="The PET domain of Prickle; cd09827"
                     /db_xref="CDD:193602"
     Region          343..400
                     /region_name="LIM1_Testin_like"
                     /note="The first LIM domain of Testin-like family;
                     cd09340"
                     /db_xref="CDD:188726"
     Site            order(343,346,369,372,375,378,396,399)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188726"
     Region          406..461
                     /region_name="LIM2_Prickle"
                     /note="The second LIM domain of Prickle; cd09418"
                     /db_xref="CDD:188802"
     Site            order(408,411,429,432,435,438,456,459)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188802"
     Region          466..524
                     /region_name="LIM3_Prickle"
                     /note="The third LIM domain of Prickle; cd09420"
                     /db_xref="CDD:188804"
     Site            order(468,471,490,495,498,501,519,523)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188804"
     CDS             1..883
                     /gene="LOC117603625"
                     /coded_by="XM_034322955.2:1616..4267"
                     /db_xref="GeneID:117603625"
ORIGIN      
        1 msslarrrkt svlgskrwwq ggcwatrshq eaylrrlyar kneqtgkged eaagrrdlga
       61 swasgtvyel ppteehylda flrnredgne eddvvgvvvg cpalatsrss ptekcvefln
      121 gahdaaisfe ndrrleepec sraadcsslh sgydvaatsl ekvcrscrcp reehqrtste
      181 aggpsglglg pgppmamama fpsgaagssh qepfkspmqa appglalqea lmhhqrhsqs
      241 dddsgcalee ytwvppglrp dqvhlyfsal pedkipyags agerervkql lqqlpphdne
      301 arycsgltee ekrelrvfaa qrkrealgrg hasqldrphg agcrecsrpi aagemavaas
      361 ragpsalwhp acfvccvcrq llvdliyfwr dgrlycgrhh aetlkprcca cdeiiladec
      421 teaegrawhm rhfaclecdr qlggqryvmr egrpyclrcf dasfaeycds cgepigvdqg
      481 qmshegqhwh atetcfccat crtsllgrpf lprrgaiycs iacskgeppt tpsdssagpp
      541 ppppsflrtg rrqpptsegs sspppppppp pppgsrhtls sprnsprmtr khhqtstsla
      601 ttptqstlsp eftaegfpss gsrpsgldrv llernlerll qertshpeen rselgrlmqa
      661 rdreplrcvq ncapshassm pelpppprps sgasasasts tstgavgvms ggalaqesat
      721 lptnliigqm dpptptsrrv rfqgdldvtd hnassrvpls pgnernssss pspppaslsr
      781 tnvsrsrslq peevastsaw gvagssksrm dgedddvesc cstcssstss deaaayalpp
      841 rrayggvris yvpndavaca rkrtptsttp nqgqrdsekc vvs
//
LOCUS       XP_034178855             764 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603625 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178855
VERSION     XP_034178855.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322964.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178855.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..764
                     /product="uncharacterized protein LOC117603625 isoform X2"
                     /calculated_mol_wt=82299
     Region          121..217
                     /region_name="PET_Prickle"
                     /note="The PET domain of Prickle; cd09827"
                     /db_xref="CDD:193602"
     Region          224..281
                     /region_name="LIM1_Testin_like"
                     /note="The first LIM domain of Testin-like family;
                     cd09340"
                     /db_xref="CDD:188726"
     Site            order(224,227,250,253,256,259,277,280)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188726"
     Region          287..342
                     /region_name="LIM2_Prickle"
                     /note="The second LIM domain of Prickle; cd09418"
                     /db_xref="CDD:188802"
     Site            order(289,292,310,313,316,319,337,340)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188802"
     Region          347..405
                     /region_name="LIM3_Prickle"
                     /note="The third LIM domain of Prickle; cd09420"
                     /db_xref="CDD:188804"
     Site            order(349,352,371,376,379,382,400,404)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188804"
     CDS             1..764
                     /gene="LOC117603625"
                     /coded_by="XM_034322964.2:1239..3533"
                     /db_xref="GeneID:117603625"
ORIGIN      
        1 mlspgqegqp nsyshgnvvl qgapcgqcrd lcpagyvphf wrkvcrscrc preehqrtst
       61 eaggpsglgl gpgppmamam afpsgaagss hqepfkspmq aappglalqe almhhqrhsq
      121 sdddsgcale eytwvppglr pdqvhlyfsa lpedkipyag sagerervkq llqqlpphdn
      181 earycsglte eekrelrvfa aqrkrealgr ghasqldrph gagcrecsrp iaagemavaa
      241 sragpsalwh pacfvccvcr qllvdliyfw rdgrlycgrh haetlkprcc acdeiilade
      301 cteaegrawh mrhfaclecd rqlggqryvm regrpyclrc fdasfaeycd scgepigvdq
      361 gqmshegqhw hatetcfcca tcrtsllgrp flprrgaiyc siacskgepp ttpsdssagp
      421 pppppsflrt grrqpptseg sssppppppp ppppgsrhtl ssprnsprmt rkhhqtstsl
      481 attptqstls peftaegfps sgsrpsgldr vllernlerl lqertshpee nrselgrlmq
      541 ardreplrcv qncapshass mpelpppprp ssgasasast ststgavgvm sggalaqesa
      601 tlptnliigq mdpptptsrr vrfqgdldvt dhnassrvpl spgnernsss spspppasls
      661 rtnvsrsrsl qpeevastsa wgvagssksr mdgedddves ccstcsssts sdeaaayalp
      721 prrayggvri syvpndavac arkrtptstt pnqgqrdsek cvvs
//
LOCUS       XP_034178863             764 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603625 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178863
VERSION     XP_034178863.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322972.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178863.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..764
                     /product="uncharacterized protein LOC117603625 isoform X2"
                     /calculated_mol_wt=82299
     Region          121..217
                     /region_name="PET_Prickle"
                     /note="The PET domain of Prickle; cd09827"
                     /db_xref="CDD:193602"
     Region          224..281
                     /region_name="LIM1_Testin_like"
                     /note="The first LIM domain of Testin-like family;
                     cd09340"
                     /db_xref="CDD:188726"
     Site            order(224,227,250,253,256,259,277,280)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188726"
     Region          287..342
                     /region_name="LIM2_Prickle"
                     /note="The second LIM domain of Prickle; cd09418"
                     /db_xref="CDD:188802"
     Site            order(289,292,310,313,316,319,337,340)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188802"
     Region          347..405
                     /region_name="LIM3_Prickle"
                     /note="The third LIM domain of Prickle; cd09420"
                     /db_xref="CDD:188804"
     Site            order(349,352,371,376,379,382,400,404)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188804"
     CDS             1..764
                     /gene="LOC117603625"
                     /coded_by="XM_034322972.2:641..2935"
                     /db_xref="GeneID:117603625"
ORIGIN      
        1 mlspgqegqp nsyshgnvvl qgapcgqcrd lcpagyvphf wrkvcrscrc preehqrtst
       61 eaggpsglgl gpgppmamam afpsgaagss hqepfkspmq aappglalqe almhhqrhsq
      121 sdddsgcale eytwvppglr pdqvhlyfsa lpedkipyag sagerervkq llqqlpphdn
      181 earycsglte eekrelrvfa aqrkrealgr ghasqldrph gagcrecsrp iaagemavaa
      241 sragpsalwh pacfvccvcr qllvdliyfw rdgrlycgrh haetlkprcc acdeiilade
      301 cteaegrawh mrhfaclecd rqlggqryvm regrpyclrc fdasfaeycd scgepigvdq
      361 gqmshegqhw hatetcfcca tcrtsllgrp flprrgaiyc siacskgepp ttpsdssagp
      421 pppppsflrt grrqpptseg sssppppppp ppppgsrhtl ssprnsprmt rkhhqtstsl
      481 attptqstls peftaegfps sgsrpsgldr vllernlerl lqertshpee nrselgrlmq
      541 ardreplrcv qncapshass mpelpppprp ssgasasast ststgavgvm sggalaqesa
      601 tlptnliigq mdpptptsrr vrfqgdldvt dhnassrvpl spgnernsss spspppasls
      661 rtnvsrsrsl qpeevastsa wgvagssksr mdgedddves ccstcsssts sdeaaayalp
      721 prrayggvri syvpndavac arkrtptstt pnqgqrdsek cvvs
//
LOCUS       XP_034178872             755 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603625 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178872
VERSION     XP_034178872.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322981.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178872.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..755
                     /product="uncharacterized protein LOC117603625 isoform X3"
                     /calculated_mol_wt=81760
     Region          112..208
                     /region_name="PET_Prickle"
                     /note="The PET domain of Prickle; cd09827"
                     /db_xref="CDD:193602"
     Region          215..272
                     /region_name="LIM1_Testin_like"
                     /note="The first LIM domain of Testin-like family;
                     cd09340"
                     /db_xref="CDD:188726"
     Site            order(215,218,241,244,247,250,268,271)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188726"
     Region          278..333
                     /region_name="LIM2_Prickle"
                     /note="The second LIM domain of Prickle; cd09418"
                     /db_xref="CDD:188802"
     Site            order(280,283,301,304,307,310,328,331)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188802"
     Region          338..396
                     /region_name="LIM3_Prickle"
                     /note="The third LIM domain of Prickle; cd09420"
                     /db_xref="CDD:188804"
     Site            order(340,343,362,367,370,373,391,395)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188804"
     CDS             1..755
                     /gene="LOC117603625"
                     /coded_by="XM_034322981.2:4179..6446"
                     /db_xref="GeneID:117603625"
ORIGIN      
        1 mrrlsgthfy tgervrwqmi nvqhsrigfa nirkvcrscr cpreehqrts teaggpsglg
       61 lgpgppmama mafpsgaags shqepfkspm qaappglalq ealmhhqrhs qsdddsgcal
      121 eeytwvppgl rpdqvhlyfs alpedkipya gsagerervk qllqqlpphd nearycsglt
      181 eeekrelrvf aaqrkrealg rghasqldrp hgagcrecsr piaagemava asragpsalw
      241 hpacfvccvc rqllvdliyf wrdgrlycgr hhaetlkprc cacdeiilad ecteaegraw
      301 hmrhfaclec drqlggqryv mregrpyclr cfdasfaeyc dscgepigvd qgqmshegqh
      361 whatetcfcc atcrtsllgr pflprrgaiy csiacskgep pttpsdssag ppppppsflr
      421 tgrrqpptse gssspppppp pppppgsrht lssprnsprm trkhhqtsts lattptqstl
      481 speftaegfp ssgsrpsgld rvllernler llqertshpe enrselgrlm qardreplrc
      541 vqncapshas smpelppppr pssgasasas tststgavgv msggalaqes atlptnliig
      601 qmdpptptsr rvrfqgdldv tdhnassrvp lspgnernss sspspppasl srtnvsrsrs
      661 lqpeevasts awgvagssks rmdgedddve sccstcssst ssdeaaayal pprrayggvr
      721 isyvpndava carkrtptst tpnqgqrdse kcvvs
//
LOCUS       XP_076546139             704 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603625 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546139
VERSION     XP_076546139.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690024.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..704
                     /product="uncharacterized protein LOC117603625 isoform X4"
                     /calculated_mol_wt=75351
     Region          240..>262
                     /region_name="PET"
                     /note="PET ((Prickle Espinas Testin) domain is involved in
                     protein-protein interactions; cl05674"
                     /db_xref="CDD:471291"
     Region          <262..282
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Region          287..345
                     /region_name="LIM3_Prickle"
                     /note="The third LIM domain of Prickle; cd09420"
                     /db_xref="CDD:188804"
     Site            order(289,292,311,316,319,322,340,344)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188804"
     CDS             1..704
                     /gene="LOC117603625"
                     /coded_by="XM_076690024.1:1626..3740"
                     /db_xref="GeneID:117603625"
ORIGIN      
        1 msslarrrkt svlgskrwwq ggcwatrshq eaylrrlyar kneqtgkged eaagrrdlga
       61 swasgtvyel ppteehylda flrnredgne eddvvgvvvg cpalatsrss ptekcvefln
      121 gahdaaisfe ndrrleepec sraadcsslh sgydvaatsl ekvcrscrcp reehqrtste
      181 aggpsglglg pgppmamama fpsgaagssh qepfkspmqa appglalqea lmhhqrhsqs
      241 dddsgcalee ytwvppglrp dqlggqryvm regrpyclrc fdasfaeycd scgepigvdq
      301 gqmshegqhw hatetcfcca tcrtsllgrp flprrgaiyc siacskgepp ttpsdssagp
      361 pppppsflrt grrqpptseg sssppppppp ppppgsrhtl ssprnsprmt rkhhqtstsl
      421 attptqstls peftaegfps sgsrpsgldr vllernlerl lqertshpee nrselgrlmq
      481 ardreplrcv qncapshass mpelpppprp ssgasasast ststgavgvm sggalaqesa
      541 tlptnliigq mdpptptsrr vrfqgdldvt dhnassrvpl spgnernsss spspppasls
      601 rtnvsrsrsl qpeevastsa wgvagssksr mdgedddves ccstcsssts sdeaaayalp
      661 prrayggvri syvpndavac arkrtptstt pnqgqrdsek cvvs
//
LOCUS       XP_034178883             824 aa            linear   INV 03-SEP-2025
DEFINITION  calpain-7 [Osmia lignaria lignaria].
ACCESSION   XP_034178883
VERSION     XP_034178883.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322992.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..824
                     /product="calpain-7"
                     /calculated_mol_wt=94117
     Region          4..67
                     /region_name="MIT"
                     /note="domain contained within Microtubule Interacting and
                     Trafficking molecules. The MIT domain is found in sorting
                     nexins, the nuclear thiol protease PalBH, the AAA protein
                     spastin and archaebacterial proteins with similar domain
                     architecture, vacuolar...; cl00299"
                     /db_xref="CDD:469712"
     Region          79..142
                     /region_name="MIT"
                     /note="domain contained within Microtubule Interacting and
                     Trafficking molecules. The MIT domain is found in sorting
                     nexins, the nuclear thiol protease PalBH, the AAA protein
                     spastin and archaebacterial proteins with similar domain
                     architecture, vacuolar...; cl00299"
                     /db_xref="CDD:469712"
     Region          230..554
                     /region_name="CysPc"
                     /note="Calpains, domains IIa, IIb; calcium-dependent
                     cytoplasmic cysteine proteinases, papain-like. Functions
                     in cytoskeletal remodeling processes, cell
                     differentiation, apoptosis and signal transduction;
                     cl00051"
                     /db_xref="CDD:469591"
     Site            order(299,467,487)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238004"
     Region          702..810
                     /region_name="Calpain_III"
                     /note="Calpain, subdomain III. Calpains are
                     calcium-activated cytoplasmic cysteine proteinases,
                     participate in cytoskeletal remodeling processes, cell
                     differentiation, apoptosis and signal transduction.
                     Catalytic domain and the two calmodulin-like domains
                     are...; cl00165"
                     /db_xref="CDD:469636"
     CDS             1..824
                     /gene="LOC117603647"
                     /coded_by="XM_034322992.2:261..2735"
                     /db_xref="GeneID:117603647"
ORIGIN      
        1 mtledvqnaa rkavqfdsns qykqalyyyn iavkyltelq dpvydqklse yqerisviek
       61 liseeeqksc kaqsvhqsel qrckflinqa qdadeaglkd iavklytdaa efglsvkttd
      121 seakgkltal vrlaldraes lkgvkkqenv diietlsklp pvpetslded vdkipslptt
      181 rpilnsgkqt rpplhrgssm hlkvsggssn yteeekrvll hsshindhef vpfmsidlte
      241 kfqyaipftd rdgllelapk qkpdfarwcr peelfsdpkm lkgphvdyys ikqtvvsdcs
      301 fvaslavsaq yekrfgrrli tsiiypknrn kepiynpfgk ymvklhingi prkviiddll
      361 pvsrynqllc syssnqgelw isllekaymk vmggydfpgs nsnidlhalt gwiperwair
      421 pgepdfnkdn lynvllsrlh kgdvlvtvat gelsdldadr tglvpthaya vlnvrninge
      481 rllqlknpws hlrwkgnyse ldtmhwtnel kealnydpds asqfdngvfw idydsicrff
      541 dvfylnwnpg lfnytycihq mwnagigpvk daynigdnpq fslevqnnvt gviwilltrh
      601 itdiadfrqn qeyitilvyk ndgrrvyyph dpppyidgvr insphylcki kldgnsdsry
      661 alvisqyekt ntiyytlray gtcpftlrkm pqlykyekei tdgqwkgvsa ggcsnhptyq
      721 nnpryqlvle ssnnnnylli ilkgpkqyqi gfdivsvvln dpdapsafkm kssgpfrsgf
      781 vyleledvpa gtyhvtpsty ipgqegpffl icksscklql qrlq
//
LOCUS       XP_034178896             714 aa            linear   INV 03-SEP-2025
DEFINITION  proto-oncogene like protein Myb isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178896
VERSION     XP_034178896.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323005.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178896.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..714
                     /product="proto-oncogene like protein Myb isoform X1"
                     /calculated_mol_wt=79176
     Region          70..>197
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          136..>236
                     /region_name="PLN03212"
                     /note="Transcription repressor MYB5; Provisional"
                     /db_xref="CDD:178751"
     Region          <375..>501
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          449..578
                     /region_name="Cmyb_C"
                     /note="C-myb, C-terminal; pfam09316"
                     /db_xref="CDD:462753"
     CDS             1..714
                     /gene="Myb"
                     /coded_by="XM_034323005.2:3520..5664"
                     /db_xref="GeneID:117603656"
ORIGIN      
        1 mdvvssgllm pakvvditty hssfyspppt easrsgydss sggesdneys asvcrgtgnq
       61 sqnatasnan shgqgkhink grwtkeedal lkqlvsnaeq rgtglrwdai aghfpdrsdv
      121 qcqqrwakvv npelvkgpwt keedekvvel verygpkkwt liarhlkgri gkqcrerwhn
      181 hlnpgikkta wteaedriiv eahrrvgnqw akiakllpgr tdnaiknhwn stmrrkyetd
      241 egrtttgtrg rgrrktvess vtskdtlcte edisarlkaa stsnknsstv tsvhtmdmdq
      301 idwarawdnq qsgqtyqgll glnerthgne sridspskrg gsvriktepi saftkyfdmq
      361 mqgeatssks teirlmpmpd leeisnsmte kersspppil rrrrntqtlq qrtdtpdsgs
      421 qseyliisqq qeeqpadntp stpikqlpfs psqflnslsp etsswprast pkgsspgplt
      481 tpqpsglrrs qndgntprtp tpfknalael errsgattql patpsrldal teiikqetdr
      541 eslagtstil qdsgygtirr rgkensapgg krarkalcqa wanasqdnse msfvvetpsk
      601 sldtsvlfsp asmalednfl tagtspvkts hdfasvqrkp nakraitfda fdspccilsp
      661 lplrpvkrak lhleaqwttv acgrtrdqle mtqaarrfls shgylplrpr slnf
//
LOCUS       XP_034178903             691 aa            linear   INV 03-SEP-2025
DEFINITION  proto-oncogene like protein Myb isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178903
VERSION     XP_034178903.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323012.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178903.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..691
                     /product="proto-oncogene like protein Myb isoform X2"
                     /calculated_mol_wt=76975
     Region          47..>174
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          113..>213
                     /region_name="PLN03212"
                     /note="Transcription repressor MYB5; Provisional"
                     /db_xref="CDD:178751"
     Region          <352..>478
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          426..555
                     /region_name="Cmyb_C"
                     /note="C-myb, C-terminal; pfam09316"
                     /db_xref="CDD:462753"
     CDS             1..691
                     /gene="Myb"
                     /coded_by="XM_034323012.2:448..2523"
                     /db_xref="GeneID:117603656"
ORIGIN      
        1 mdmwfarkrs rsgydsssgg esdneysasv crgtgnqsqn atasnanshg qgkhinkgrw
       61 tkeedallkq lvsnaeqrgt glrwdaiagh fpdrsdvqcq qrwakvvnpe lvkgpwtkee
      121 dekvvelver ygpkkwtlia rhlkgrigkq crerwhnhln pgikktawte aedriiveah
      181 rrvgnqwaki akllpgrtdn aiknhwnstm rrkyetdegr tttgtrgrgr rktvessvts
      241 kdtlcteedi sarlkaasts nknsstvtsv htmdmdqidw arawdnqqsg qtyqgllgln
      301 erthgnesri dspskrggsv riktepisaf tkyfdmqmqg eatsskstei rlmpmpdlee
      361 isnsmteker sspppilrrr rntqtlqqrt dtpdsgsqse yliisqqqee qpadntpstp
      421 ikqlpfspsq flnslspets swprastpkg sspgplttpq psglrrsqnd gntprtptpf
      481 knalaelerr sgattqlpat psrldaltei ikqetdresl agtstilqds gygtirrrgk
      541 ensapggkra rkalcqawan asqdnsemsf vvetpsksld tsvlfspasm alednfltag
      601 tspvktshdf asvqrkpnak raitfdafds pccilsplpl rpvkraklhl eaqwttvacg
      661 rtrdqlemtq aarrflsshg ylplrprsln f
//
LOCUS       XP_034178911             691 aa            linear   INV 03-SEP-2025
DEFINITION  proto-oncogene like protein Myb isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178911
VERSION     XP_034178911.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178911.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..691
                     /product="proto-oncogene like protein Myb isoform X2"
                     /calculated_mol_wt=76975
     Region          47..>174
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          113..>213
                     /region_name="PLN03212"
                     /note="Transcription repressor MYB5; Provisional"
                     /db_xref="CDD:178751"
     Region          <352..>478
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          426..555
                     /region_name="Cmyb_C"
                     /note="C-myb, C-terminal; pfam09316"
                     /db_xref="CDD:462753"
     CDS             1..691
                     /gene="Myb"
                     /coded_by="XM_034323020.2:260..2335"
                     /db_xref="GeneID:117603656"
ORIGIN      
        1 mdmwfarkrs rsgydsssgg esdneysasv crgtgnqsqn atasnanshg qgkhinkgrw
       61 tkeedallkq lvsnaeqrgt glrwdaiagh fpdrsdvqcq qrwakvvnpe lvkgpwtkee
      121 dekvvelver ygpkkwtlia rhlkgrigkq crerwhnhln pgikktawte aedriiveah
      181 rrvgnqwaki akllpgrtdn aiknhwnstm rrkyetdegr tttgtrgrgr rktvessvts
      241 kdtlcteedi sarlkaasts nknsstvtsv htmdmdqidw arawdnqqsg qtyqgllgln
      301 erthgnesri dspskrggsv riktepisaf tkyfdmqmqg eatsskstei rlmpmpdlee
      361 isnsmteker sspppilrrr rntqtlqqrt dtpdsgsqse yliisqqqee qpadntpstp
      421 ikqlpfspsq flnslspets swprastpkg sspgplttpq psglrrsqnd gntprtptpf
      481 knalaelerr sgattqlpat psrldaltei ikqetdresl agtstilqds gygtirrrgk
      541 ensapggkra rkalcqawan asqdnsemsf vvetpsksld tsvlfspasm alednfltag
      601 tspvktshdf asvqrkpnak raitfdafds pccilsplpl rpvkraklhl eaqwttvacg
      661 rtrdqlemtq aarrflsshg ylplrprsln f
//
LOCUS       XP_034178939             672 aa            linear   INV 03-SEP-2025
DEFINITION  proto-oncogene like protein Myb isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178939
VERSION     XP_034178939.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323048.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178939.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..672
                     /product="proto-oncogene like protein Myb isoform X3"
                     /calculated_mol_wt=74510
     Region          70..>197
                     /region_name="REB1"
                     /note="Myb superfamily proteins, including transcription
                     factors and mRNA splicing factors [Transcription / RNA
                     processing and modification / Cell division and chromosome
                     partitioning]; COG5147"
                     /db_xref="CDD:227476"
     Region          136..>236
                     /region_name="PLN03212"
                     /note="Transcription repressor MYB5; Provisional"
                     /db_xref="CDD:178751"
     Region          <375..>501
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          449..578
                     /region_name="Cmyb_C"
                     /note="C-myb, C-terminal; pfam09316"
                     /db_xref="CDD:462753"
     CDS             1..672
                     /gene="Myb"
                     /coded_by="XM_034323048.2:4616..6634"
                     /db_xref="GeneID:117603656"
ORIGIN      
        1 mdvvssgllm pakvvditty hssfyspppt easrsgydss sggesdneys asvcrgtgnq
       61 sqnatasnan shgqgkhink grwtkeedal lkqlvsnaeq rgtglrwdai aghfpdrsdv
      121 qcqqrwakvv npelvkgpwt keedekvvel verygpkkwt liarhlkgri gkqcrerwhn
      181 hlnpgikkta wteaedriiv eahrrvgnqw akiakllpgr tdnaiknhwn stmrrkyetd
      241 egrtttgtrg rgrrktvess vtskdtlcte edisarlkaa stsnknsstv tsvhtmdmdq
      301 idwarawdnq qsgqtyqgll glnerthgne sridspskrg gsvriktepi saftkyfdmq
      361 mqgeatssks teirlmpmpd leeisnsmte kersspppil rrrrntqtlq qrtdtpdsgs
      421 qseyliisqq qeeqpadntp stpikqlpfs psqflnslsp etsswprast pkgsspgplt
      481 tpqpsglrrs qndgntprtp tpfknalael errsgattql patpsrldal teiikqetdr
      541 eslagtstil qdsgygtirr rgkensapgg krarkalcqa wanasqdnse msfvvetpsk
      601 sldtsvlfsp asmalednfl tagtspvklh leaqwttvac grtrdqlemt qaarrflssh
      661 gylplrprsl nf
//
LOCUS       XP_034172018             667 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600533 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172018
VERSION     XP_034172018.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316127.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172018.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..667
                     /product="uncharacterized protein LOC117600533 isoform X1"
                     /calculated_mol_wt=75325
     Region          95..183
                     /region_name="PDZ_tamalin_CYTIP-like"
                     /note="PDZ domain of tamalin, cytohesin-1-interacting
                     protein (CYTIP), and related domains; cd06713"
                     /db_xref="CDD:467197"
     Site            order(108..114,133,136,165,169,172..173)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467197"
     CDS             1..667
                     /gene="LOC117600533"
                     /coded_by="XM_034316127.2:267..2270"
                     /db_xref="GeneID:117600533"
ORIGIN      
        1 mnnspqntev agqqqrtgve nsdeeewsgv vecvmllhrl vyrkndfyll kqpssssfll
       61 pisscfswsr kfcglhnavl erskservtr adedrrrrti ivekkngtyg ftlqsygihy
      121 kreqeiemvt yvdyveydgp afkagmregd vilsinghem dradhktlvn fikncdtrmr
      181 mvvsfedcvr kvelhmryie lqralqsrls elerlcerer silmgrwkth slparkrtpn
      241 svitsnpnqp spsssfnsst iqccrpatst ehlllynvfa dgrpclvprn aaclvaigpp
      301 rsrsdhhhfl skmssesgvt tssrhsyhqa ngmnstpaks klhkscqqqq qqsntsgdpn
      361 plhpppqnsl cvacissanr rrehsdsgsl daydlaspcc dpncvpsrrr rekqrrarne
      421 qnhhqqqqqq qqqsqvqhhh vqreqsqqqq qqqqqqqhgt hncsrtsghs lhsitssdvs
      481 taadsvascs tslstdtlyw dpsvhqrppp clqyakpksw dnlttkafgg ygfgygyldt
      541 atikthsaer pgkgahgrak tptgtvqrrt sssttysgss srhfqptkst esllipppyq
      601 seldaslsce cldgpaprci piiqmekhnr qdegsyiint htqvrhrrss hsdgklraln
      661 nsevtrl
//
LOCUS       XP_034172029             666 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600533 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172029
VERSION     XP_034172029.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172029.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..666
                     /product="uncharacterized protein LOC117600533 isoform X2"
                     /calculated_mol_wt=75226
     Region          95..183
                     /region_name="PDZ_tamalin_CYTIP-like"
                     /note="PDZ domain of tamalin, cytohesin-1-interacting
                     protein (CYTIP), and related domains; cd06713"
                     /db_xref="CDD:467197"
     Site            order(108..114,133,136,165,169,172..173)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467197"
     CDS             1..666
                     /gene="LOC117600533"
                     /coded_by="XM_034316138.2:267..2267"
                     /db_xref="GeneID:117600533"
ORIGIN      
        1 mnnspqntev agqqqrtgve nsdeeewsgv vecvmllhrl vyrkndfyll kqpssssfll
       61 pisscfswsr kfcglhnavl erskservtr adedrrrrti ivekkngtyg ftlqsygihy
      121 kreqeiemvt yvdyveydgp afkagmregd vilsinghem dradhktlvn fikncdtrmr
      181 mvvsfedcvr kvelhmryie lqralqsrls elerlcerer silmgrwkth slparkrtpn
      241 svitsnpnqp spsssfnsst iqccrpatst ehlllynfad grpclvprna aclvaigppr
      301 srsdhhhfls kmssesgvtt ssrhsyhqan gmnstpaksk lhkscqqqqq qsntsgdpnp
      361 lhpppqnslc vacissanrr rehsdsgsld aydlaspccd pncvpsrrrr ekqrrarneq
      421 nhhqqqqqqq qqsqvqhhhv qreqsqqqqq qqqqqqhgth ncsrtsghsl hsitssdvst
      481 aadsvascst slstdtlywd psvhqrpppc lqyakpkswd nlttkafggy gfgygyldta
      541 tikthsaerp gkgahgrakt ptgtvqrrts ssttysgsss rhfqptkste sllipppyqs
      601 eldaslscec ldgpaprcip iiqmekhnrq degsyiinth tqvrhrrssh sdgklralnn
      661 sevtrl
//
LOCUS       XP_034172038             623 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600533 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172038
VERSION     XP_034172038.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316147.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172038.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..623
                     /product="uncharacterized protein LOC117600533 isoform X3"
                     /calculated_mol_wt=70123
     Region          51..139
                     /region_name="PDZ_tamalin_CYTIP-like"
                     /note="PDZ domain of tamalin, cytohesin-1-interacting
                     protein (CYTIP), and related domains; cd06713"
                     /db_xref="CDD:467197"
     Site            order(64..70,89,92,121,125,128..129)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467197"
     CDS             1..623
                     /gene="LOC117600533"
                     /coded_by="XM_034316147.2:267..2138"
                     /db_xref="GeneID:117600533"
ORIGIN      
        1 mnnspqntev agqqqrtgve nsdeeewsgv vecavlersk servtraded rrrrtiivek
       61 kngtygftlq sygihykreq eiemvtyvdy veydgpafka gmregdvils inghemdrad
      121 hktlvnfikn cdtrmrmvvs fedcvrkvel hmryielqra lqsrlseler lcerersilm
      181 grwkthslpa rkrtpnsvit snpnqpspss sfnsstiqcc rpatstehll lynvfadgrp
      241 clvprnaacl vaigpprsrs dhhhflskms sesgvttssr hsyhqangmn stpaksklhk
      301 scqqqqqqsn tsgdpnplhp ppqnslcvac issanrrreh sdsgsldayd laspccdpnc
      361 vpsrrrrekq rrarneqnhh qqqqqqqqqs qvqhhhvqre qsqqqqqqqq qqqhgthncs
      421 rtsghslhsi tssdvstaad svascstsls tdtlywdpsv hqrpppclqy akpkswdnlt
      481 tkafggygfg ygyldtatik thsaerpgkg ahgraktptg tvqrrtssst tysgsssrhf
      541 qptkstesll ipppyqseld aslscecldg paprcipiiq mekhnrqdeg syiinthtqv
      601 rhrrsshsdg klralnnsev trl
//
LOCUS       XP_076546140             622 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600533 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546140
VERSION     XP_076546140.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690025.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..622
                     /product="uncharacterized protein LOC117600533 isoform X4"
                     /calculated_mol_wt=70024
     Region          51..139
                     /region_name="PDZ_tamalin_CYTIP-like"
                     /note="PDZ domain of tamalin, cytohesin-1-interacting
                     protein (CYTIP), and related domains; cd06713"
                     /db_xref="CDD:467197"
     Site            order(64..70,89,92,121,125,128..129)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467197"
     CDS             1..622
                     /gene="LOC117600533"
                     /coded_by="XM_076690025.1:267..2135"
                     /db_xref="GeneID:117600533"
ORIGIN      
        1 mnnspqntev agqqqrtgve nsdeeewsgv vecavlersk servtraded rrrrtiivek
       61 kngtygftlq sygihykreq eiemvtyvdy veydgpafka gmregdvils inghemdrad
      121 hktlvnfikn cdtrmrmvvs fedcvrkvel hmryielqra lqsrlseler lcerersilm
      181 grwkthslpa rkrtpnsvit snpnqpspss sfnsstiqcc rpatstehll lynfadgrpc
      241 lvprnaaclv aigpprsrsd hhhflskmss esgvttssrh syhqangmns tpaksklhks
      301 cqqqqqqsnt sgdpnplhpp pqnslcvaci ssanrrrehs dsgsldaydl aspccdpncv
      361 psrrrrekqr rarneqnhhq qqqqqqqqsq vqhhhvqreq sqqqqqqqqq qqhgthncsr
      421 tsghslhsit ssdvstaads vascstslst dtlywdpsvh qrpppclqya kpkswdnltt
      481 kafggygfgy gyldtatikt hsaerpgkga hgraktptgt vqrrtssstt ysgsssrhfq
      541 ptksteslli pppyqselda slscecldgp aprcipiiqm ekhnrqdegs yiinthtqvr
      601 hrrsshsdgk lralnnsevt rl
//
LOCUS       XP_034172045             597 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600533 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172045
VERSION     XP_034172045.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316154.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172045.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="uncharacterized protein LOC117600533 isoform X5"
                     /calculated_mol_wt=67225
     Region          25..113
                     /region_name="PDZ_tamalin_CYTIP-like"
                     /note="PDZ domain of tamalin, cytohesin-1-interacting
                     protein (CYTIP), and related domains; cd06713"
                     /db_xref="CDD:467197"
     Site            order(38..44,63,66,95,99,102..103)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467197"
     CDS             1..597
                     /gene="LOC117600533"
                     /coded_by="XM_034316154.2:272..2065"
                     /db_xref="GeneID:117600533"
ORIGIN      
        1 mqsscisavl erskservtr adedrrrrti ivekkngtyg ftlqsygihy kreqeiemvt
       61 yvdyveydgp afkagmregd vilsinghem dradhktlvn fikncdtrmr mvvsfedcvr
      121 kvelhmryie lqralqsrls elerlcerer silmgrwkth slparkrtpn svitsnpnqp
      181 spsssfnsst iqccrpatst ehlllynvfa dgrpclvprn aaclvaigpp rsrsdhhhfl
      241 skmssesgvt tssrhsyhqa ngmnstpaks klhkscqqqq qqsntsgdpn plhpppqnsl
      301 cvacissanr rrehsdsgsl daydlaspcc dpncvpsrrr rekqrrarne qnhhqqqqqq
      361 qqqsqvqhhh vqreqsqqqq qqqqqqqhgt hncsrtsghs lhsitssdvs taadsvascs
      421 tslstdtlyw dpsvhqrppp clqyakpksw dnlttkafgg ygfgygyldt atikthsaer
      481 pgkgahgrak tptgtvqrrt sssttysgss srhfqptkst esllipppyq seldaslsce
      541 cldgpaprci piiqmekhnr qdegsyiint htqvrhrrss hsdgklraln nsevtrl
//
LOCUS       XP_034172063             540 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600533 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172063
VERSION     XP_034172063.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172063.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="uncharacterized protein LOC117600533 isoform X6"
                     /calculated_mol_wt=60492
     Region          <1..56
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     CDS             1..540
                     /gene="LOC117600533"
                     /coded_by="XM_034316172.2:313..1935"
                     /db_xref="GeneID:117600533"
ORIGIN      
        1 mvtyvdyvey dgpafkagmr egdvilsing hemdradhkt lvnfikncdt rmrmvvsfed
       61 cvrkvelhmr yielqralqs rlselerlce rersilmgrw kthslparkr tpnsvitsnp
      121 nqpspsssfn sstiqccrpa tstehlllyn vfadgrpclv prnaaclvai gpprsrsdhh
      181 hflskmsses gvttssrhsy hqangmnstp aksklhkscq qqqqqsntsg dpnplhpppq
      241 nslcvaciss anrrrehsds gsldaydlas pccdpncvps rrrrekqrra rneqnhhqqq
      301 qqqqqqsqvq hhhvqreqsq qqqqqqqqqq hgthncsrts ghslhsitss dvstaadsva
      361 scstslstdt lywdpsvhqr pppclqyakp kswdnlttka fggygfgygy ldtatikths
      421 aerpgkgahg raktptgtvq rrtsssttys gsssrhfqpt kstesllipp pyqseldasl
      481 scecldgpap rcipiiqmek hnrqdegsyi inthtqvrhr rsshsdgklr alnnsevtrl
//
LOCUS       XP_034172064             607 aa            linear   INV 03-SEP-2025
DEFINITION  poly(A) specific ribonuclease subunit PAN3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034172064
VERSION     XP_034172064.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316173.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..607
                     /product="poly(A) specific ribonuclease subunit PAN3
                     isoform X1"
                     /calculated_mol_wt=67216
     Region          214..>409
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Region          462..599
                     /region_name="Pan3_PK"
                     /note="Pan3 Pseudokinase domain; pfam18101"
                     /db_xref="CDD:465649"
     CDS             1..607
                     /gene="PAN3"
                     /coded_by="XM_034316173.2:66..1889"
                     /db_xref="GeneID:117600563"
ORIGIN      
        1 mdpsmfvayt pqtngvples klatymnrqs pgitlstsav tkhlsnlsld sqkkvtaspe
       61 fvpgrgltss nssspnlfnn syhsqenvgg ttyfylgnts aesvgaeegt etigtvgagq
      121 vgyvypgtpa hlqpvkptkp pssnnssaps tpppqatpsf fvsenlrmdi lqknaltlaq
      181 pdvvrfpdlp nevdnyhelc plepihkpas tilgyqtsty katsiksgtr yclrrihdfr
      241 lantkcmvlv dmwkrlshtn lvqlrevftt kafgdhsmvf vydyhpgset llnkhfsate
      301 lngytdpfss dpnaprpysh tkntilrqqh ssmlpesviw syiiqltaal rvihaaglay
      361 rcldptkvll tsrtrlrlsc vaipdvvtfd gsaanplsli phyqqedlia lgklvlalac
      421 rsllavhrdn mqaslelvar systdlrnli lyllsnqarr svtdlmpmig arfytqldaa
      481 qlrsdvlene lakelengrl fkllvklati nerpelnmep twaetgdrym lklfrdyvfh
      541 qvaadgrpwl dmahvvscln kldsgsqdki clmsrdeqsv lvvsyaelrq cletsfgelv
      601 qsakdav
//
LOCUS       XP_034172065             581 aa            linear   INV 03-SEP-2025
DEFINITION  poly(A) specific ribonuclease subunit PAN3 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034172065
VERSION     XP_034172065.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316174.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="poly(A) specific ribonuclease subunit PAN3
                     isoform X2"
                     /calculated_mol_wt=64479
     Region          188..>383
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Region          436..573
                     /region_name="Pan3_PK"
                     /note="Pan3 Pseudokinase domain; pfam18101"
                     /db_xref="CDD:465649"
     CDS             1..581
                     /gene="PAN3"
                     /coded_by="XM_034316174.2:60..1805"
                     /db_xref="GeneID:117600563"
ORIGIN      
        1 mdpsmfvayt pqtngvples klatymkkvt aspefvpgrg ltssnssspn lfnnsyhsqe
       61 nvggttyfyl gntsaesvga eegtetigtv gagqvgyvyp gtpahlqpvk ptkppssnns
      121 sapstpppqa tpsffvsenl rmdilqknal tlaqpdvvrf pdlpnevdny helcplepih
      181 kpastilgyq tstykatsik sgtryclrri hdfrlantkc mvlvdmwkrl shtnlvqlre
      241 vfttkafgdh smvfvydyhp gsetllnkhf satelngytd pfssdpnapr pyshtkntil
      301 rqqhssmlpe sviwsyiiql taalrvihaa glayrcldpt kvlltsrtrl rlscvaipdv
      361 vtfdgsaanp lsliphyqqe dlialgklvl alacrsllav hrdnmqasle lvarsystdl
      421 rnlilyllsn qarrsvtdlm pmigarfytq ldaaqlrsdv lenelakele ngrlfkllvk
      481 latinerpel nmeptwaetg drymlklfrd yvfhqvaadg rpwldmahvv sclnkldsgs
      541 qdkiclmsrd eqsvlvvsya elrqcletsf gelvqsakda v
//
LOCUS       XP_076546141             545 aa            linear   INV 03-SEP-2025
DEFINITION  poly(A) specific ribonuclease subunit PAN3 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546141
VERSION     XP_076546141.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690026.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..545
                     /product="poly(A) specific ribonuclease subunit PAN3
                     isoform X3"
                     /calculated_mol_wt=61095
     Region          152..>347
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          400..537
                     /region_name="Pan3_PK"
                     /note="Pan3 Pseudokinase domain; pfam18101"
                     /db_xref="CDD:465649"
     CDS             1..545
                     /gene="PAN3"
                     /coded_by="XM_076690026.1:115..1752"
                     /db_xref="GeneID:117600563"
ORIGIN      
        1 miyllyifwi essksrdnvk ylscyetfvk pfsgftekae svgaeegtet igtvgagqvg
       61 yvypgtpahl qpvkptkpps snnssapstp ppqatpsffv senlrmdilq knaltlaqpd
      121 vvrfpdlpne vdnyhelcpl epihkpasti lgyqtstyka tsiksgtryc lrrihdfrla
      181 ntkcmvlvdm wkrlshtnlv qlrevfttka fgdhsmvfvy dyhpgsetll nkhfsateln
      241 gytdpfssdp naprpyshtk ntilrqqhss mlpesviwsy iiqltaalrv ihaaglayrc
      301 ldptkvllts rtrlrlscva ipdvvtfdgs aanplsliph yqqedlialg klvlalacrs
      361 llavhrdnmq aslelvarsy stdlrnlily llsnqarrsv tdlmpmigar fytqldaaql
      421 rsdvlenela kelengrlfk llvklatine rpelnmeptw aetgdrymlk lfrdyvfhqv
      481 aadgrpwldm ahvvsclnkl dsgsqdkicl msrdeqsvlv vsyaelrqcl etsfgelvqs
      541 akdav
//
LOCUS       XP_076546142             586 aa            linear   INV 03-SEP-2025
DEFINITION  PX domain-containing protein kinase-like protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546142
VERSION     XP_076546142.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690027.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="PX domain-containing protein kinase-like protein
                     isoform X1"
                     /calculated_mol_wt=65891
     Region          13..130
                     /region_name="PX_MONaKA"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Modulator of Na,K-ATPase; cd06871"
                     /db_xref="CDD:132781"
     Site            order(55..57,78..79,92)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132781"
     Region          204..399
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          560..>581
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(562..563,565..567,569..571,578..581)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..586
                     /gene="LOC117603700"
                     /coded_by="XM_076690027.1:290..2050"
                     /db_xref="GeneID:117603700"
ORIGIN      
        1 malfekrytn kvllddtekl tsvienarti dghteyvikt qrgpipeksw rvsrryndfv
       61 qlngalsisg idlalppkki ignmepdfia qrqvalqnyl nnilmnpila sslpmkkfld
      121 pdnytaplhd srnfleialq qvslalrsda nfevckaipd mgwrlrkhyy tvknrqnpkq
      181 elllawvefg pdkhlqdkdm qgvfkslgtl khsyiepivy qhvtesgalm irnfhptgtl
      241 rdtlcvtkpk qpfikkygnp kqtksltvpe iamyghqile algflhekgl pyghlhtgni
      301 lltqrcaklf diengllglp afyrpyvvqr rklhattqvd iysfghvlye mafgrpllea
      361 tcselphcda tlrsllevil spealkqdlp tihhllehpf fegarraama vgsiekphfk
      421 lsshlkealq eavnkaeqrl kdeqkvarhq krlvkvqemm sseeemkkrk qklkkeqkla
      481 qeqrsanqlg tngmkhvngk spersespts tstatsvgtl tppslrsvdg phsnggpvkg
      541 avppppsqpi nlvdnvpkvt neraallgsi snfnkaslrk vsngyn
//
LOCUS       XP_076546143             583 aa            linear   INV 03-SEP-2025
DEFINITION  PX domain-containing protein kinase-like protein isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546143
VERSION     XP_076546143.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690028.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..583
                     /product="PX domain-containing protein kinase-like protein
                     isoform X2"
                     /calculated_mol_wt=65571
     Region          13..130
                     /region_name="PX_MONaKA"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Modulator of Na,K-ATPase; cd06871"
                     /db_xref="CDD:132781"
     Site            order(55..57,78..79,92)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132781"
     Region          204..399
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          557..>578
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(559..560,562..564,566..568,575..578)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..583
                     /gene="LOC117603700"
                     /coded_by="XM_076690028.1:291..2042"
                     /db_xref="GeneID:117603700"
ORIGIN      
        1 malfekrytn kvllddtekl tsvienarti dghteyvikt qrgpipeksw rvsrryndfv
       61 qlngalsisg idlalppkki ignmepdfia qrqvalqnyl nnilmnpila sslpmkkfld
      121 pdnytaplhd srnfleialq qvslalrsda nfevckaipd mgwrlrkhyy tvknrqnpkq
      181 elllawvefg pdkhlqdkdm qgvfkslgtl khsyiepivy qhvtesgalm irnfhptgtl
      241 rdtlcvtkpk qpfikkygnp kqtksltvpe iamyghqile algflhekgl pyghlhtgni
      301 lltqrcaklf diengllglp afyrpyvvqr rklhattqvd iysfghvlye mafgrpllea
      361 tcselphcda tlrsllevil spealkqdlp tihhllehpf fegarraama vgsiekphfk
      421 lsshlkealq eavnkaeqrl kdeqkvarhq krlvkvqemm sseeemkkrk qklkkeqkla
      481 qeqrsanqlg tngmkhvngk spersespts tstatsvgtl tppslhgphs nggpvkgavp
      541 pppsqpinlv dnvpkvtner aallgsisnf nkaslrkvsn gyn
//
LOCUS       XP_034178989             580 aa            linear   INV 03-SEP-2025
DEFINITION  PX domain-containing protein kinase-like protein isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034178989
VERSION     XP_034178989.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178989.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="PX domain-containing protein kinase-like protein
                     isoform X3"
                     /calculated_mol_wt=65158
     Region          13..132
                     /region_name="PX_MONaKA"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Modulator of Na,K-ATPase; cd06871"
                     /db_xref="CDD:132781"
     Site            order(55..57,78..79,92)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132781"
     Region          198..393
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          554..>575
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(556..557,559..561,563..565,572..575)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..580
                     /gene="LOC117603700"
                     /coded_by="XM_034323098.2:290..2032"
                     /db_xref="GeneID:117603700"
ORIGIN      
        1 malfekrytn kvllddtekl tsvienarti dghteyvikt qrgpipeksw rvsrryndfv
       61 qlngalsisg idlalppkki ignmepdfia qrqvalqnyl nnilmnpila sslpmkkfld
      121 pdnytaplhe ialqqvslal rsdanfevck aipdmgwrlr khyytvknrq npkqelllaw
      181 vefgpdkhlq dkdmqgvfks lgtlkhsyie pivyqhvtes galmirnfhp tgtlrdtlcv
      241 tkpkqpfikk ygnpkqtksl tvpeiamygh qilealgflh ekglpyghlh tgnilltqrc
      301 aklfdiengl lglpafyrpy vvqrrklhat tqvdiysfgh vlyemafgrp lleatcselp
      361 hcdatlrsll evilspealk qdlptihhll ehpffegarr aamavgsiek phfklsshlk
      421 ealqeavnka eqrlkdeqkv arhqkrlvkv qemmsseeem kkrkqklkke qklaqeqrsa
      481 nqlgtngmkh vngksperse sptststats vgtltppslr svdgphsngg pvkgavpppp
      541 sqpinlvdnv pkvtneraal lgsisnfnka slrkvsngyn
//
LOCUS       XP_076546144             530 aa            linear   INV 03-SEP-2025
DEFINITION  PX domain-containing protein kinase-like protein isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546144
VERSION     XP_076546144.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690029.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..530
                     /product="PX domain-containing protein kinase-like protein
                     isoform X4"
                     /calculated_mol_wt=60082
     Region          13..130
                     /region_name="PX_MONaKA"
                     /note="The phosphoinositide binding Phox Homology domain
                     of Modulator of Na,K-ATPase; cd06871"
                     /db_xref="CDD:132781"
     Site            order(55..57,78..79,92)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132781"
     Region          204..399
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..530
                     /gene="LOC117603700"
                     /coded_by="XM_076690029.1:291..1883"
                     /db_xref="GeneID:117603700"
ORIGIN      
        1 malfekrytn kvllddtekl tsvienarti dghteyvikt qrgpipeksw rvsrryndfv
       61 qlngalsisg idlalppkki ignmepdfia qrqvalqnyl nnilmnpila sslpmkkfld
      121 pdnytaplhd srnfleialq qvslalrsda nfevckaipd mgwrlrkhyy tvknrqnpkq
      181 elllawvefg pdkhlqdkdm qgvfkslgtl khsyiepivy qhvtesgalm irnfhptgtl
      241 rdtlcvtkpk qpfikkygnp kqtksltvpe iamyghqile algflhekgl pyghlhtgni
      301 lltqrcaklf diengllglp afyrpyvvqr rklhattqvd iysfghvlye mafgrpllea
      361 tcselphcda tlrsllevil spealkqdlp tihhllehpf fegarraama vgsiekphfk
      421 lsshlkealq eavnkaeqrl kdeqkvarhq krlvkvqemm sseeemkkrk qklkkeqkla
      481 qeqrsanqlg tngmkhvngk spersespts tstatsvgtl tppslpghks
//
LOCUS       XP_034179035             570 aa            linear   INV 03-SEP-2025
DEFINITION  glycosyltransferase 25 family member [Osmia lignaria lignaria].
ACCESSION   XP_034179035
VERSION     XP_034179035.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323144.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="glycosyltransferase 25 family member"
                     /calculated_mol_wt=66259
     Region          32..>78
                     /region_name="Glyco_tranf_GTA_type"
                     /note="Glycosyltransferase family A (GT-A) includes
                     diverse families of glycosyl transferases with a common
                     GT-A type structural fold; cl11394"
                     /db_xref="CDD:472172"
     Region          321..552
                     /region_name="COG3306"
                     /note="Glycosyltransferase involved in LPS biosynthesis,
                     GR25 family [Cell wall/membrane/envelope biogenesis]"
                     /db_xref="CDD:442535"
     CDS             1..570
                     /gene="LOC117603722"
                     /coded_by="XM_034323144.2:273..1985"
                     /db_xref="GeneID:117603722"
ORIGIN      
        1 mklinlvhit fvivtmflii ygrdlkkpsv liailvrnka htlpyfltfl eqlnypksri
       61 hlwirsdnni dksievlstw lsrtaneyhg vetnfneksi gfedengiah wstqrflhvi
      121 klredalnag rniwadfvwm ldadvfltna ntlnelilkn qtavapllks dglysnfwag
      181 mtsdyyylrt dkyepilfre etgcfdvpmi hsavlidlrt hlsdrltydp knlnhydgpt
      241 ddiitfavga nksgvplfic ndniygniiv pleetetiae diqrlinikt eilsnsnylp
      301 lstylehfvq ypaedtlqvd hiyminllrr qerrdrmhkl fkelgiraet idaidgrtln
      361 etvlhklgiv mmpeytdpyh erpmtmgevg cflshyaiwn kviqngykna iileddvrfe
      421 pffrqkvnyi laelnslqle wdlvylgrkr liennepwvd gskylvhaay sywtlgyils
      481 argakrlvea mplkklipvd eylpilsdvh prkdwkvhyp krdliilsan plliypthyt
      541 geqgyvsdte dstivfnqqn vnkskeredl
//
LOCUS       XP_034179059             561 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603726 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179059
VERSION     XP_034179059.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323168.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179059.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="uncharacterized protein LOC117603726 isoform X1"
                     /calculated_mol_wt=61313
     Region          455..>535
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          479..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(481,483,485,487..488,491..492,495,511,513,517..518,
                     521..522,525,539,541,543,545..546,549..550,553)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          504..526
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          532..554
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          534..554
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..561
                     /gene="LOC117603726"
                     /coded_by="XM_034323168.2:2232..3917"
                     /db_xref="GeneID:117603726"
ORIGIN      
        1 mvprgltvgr siweqavlea gpevgpepeq qrfydspppr mdvsvcclpa sagsgaqhph
       61 paslpsggqp tiqppyqyad qivpdrgqpd glpvlgctgq dnwqqisatn vagavtaatt
      121 tttyidyswl qmqqtseldt llcrqdldrl qkldeddpek dsressvqme dffevggpvc
      181 rpqasfvssr sqpagndddv flrpplwedi tssiqkldpe nadmlasqsh vkmetedvas
      241 pvlspveikt eplpppptlh llegptsnsv qtyvnghapa stdpqvvhhr tsttayktfs
      301 snnnnnnnnn nnannnnnnn tttnnnntvs vnnngngqgh ghghghghgh ghgnnnnvgn
      361 gnnnnsnsnn nnggnngsnn snndstnshg nqiggtatsp lygsmtrlmy ispltppisd
      421 pgspigappr rtppppypqp simnpshriq pspistkfnr rnnpelekrr vhhcdfigct
      481 kvytksshlk ahqrihtgek pyqcqwpece wrfarsdelt rhyrkhtgak pfkcavcers
      541 farsdhlalh mkrhlpkqha k
//
LOCUS       XP_034179068             561 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603726 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179068
VERSION     XP_034179068.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323177.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179068.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="uncharacterized protein LOC117603726 isoform X1"
                     /calculated_mol_wt=61313
     Region          455..>535
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          479..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(481,483,485,487..488,491..492,495,511,513,517..518,
                     521..522,525,539,541,543,545..546,549..550,553)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          504..526
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          532..554
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          534..554
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..561
                     /gene="LOC117603726"
                     /coded_by="XM_034323177.2:1218..2903"
                     /db_xref="GeneID:117603726"
ORIGIN      
        1 mvprgltvgr siweqavlea gpevgpepeq qrfydspppr mdvsvcclpa sagsgaqhph
       61 paslpsggqp tiqppyqyad qivpdrgqpd glpvlgctgq dnwqqisatn vagavtaatt
      121 tttyidyswl qmqqtseldt llcrqdldrl qkldeddpek dsressvqme dffevggpvc
      181 rpqasfvssr sqpagndddv flrpplwedi tssiqkldpe nadmlasqsh vkmetedvas
      241 pvlspveikt eplpppptlh llegptsnsv qtyvnghapa stdpqvvhhr tsttayktfs
      301 snnnnnnnnn nnannnnnnn tttnnnntvs vnnngngqgh ghghghghgh ghgnnnnvgn
      361 gnnnnsnsnn nnggnngsnn snndstnshg nqiggtatsp lygsmtrlmy ispltppisd
      421 pgspigappr rtppppypqp simnpshriq pspistkfnr rnnpelekrr vhhcdfigct
      481 kvytksshlk ahqrihtgek pyqcqwpece wrfarsdelt rhyrkhtgak pfkcavcers
      541 farsdhlalh mkrhlpkqha k
//
LOCUS       XP_034179076             561 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603726 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179076
VERSION     XP_034179076.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323185.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179076.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="uncharacterized protein LOC117603726 isoform X1"
                     /calculated_mol_wt=61313
     Region          455..>535
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          479..496
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(481,483,485,487..488,491..492,495,511,513,517..518,
                     521..522,525,539,541,543,545..546,549..550,553)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          504..526
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          532..554
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          534..554
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..561
                     /gene="LOC117603726"
                     /coded_by="XM_034323185.2:1883..3568"
                     /db_xref="GeneID:117603726"
ORIGIN      
        1 mvprgltvgr siweqavlea gpevgpepeq qrfydspppr mdvsvcclpa sagsgaqhph
       61 paslpsggqp tiqppyqyad qivpdrgqpd glpvlgctgq dnwqqisatn vagavtaatt
      121 tttyidyswl qmqqtseldt llcrqdldrl qkldeddpek dsressvqme dffevggpvc
      181 rpqasfvssr sqpagndddv flrpplwedi tssiqkldpe nadmlasqsh vkmetedvas
      241 pvlspveikt eplpppptlh llegptsnsv qtyvnghapa stdpqvvhhr tsttayktfs
      301 snnnnnnnnn nnannnnnnn tttnnnntvs vnnngngqgh ghghghghgh ghgnnnnvgn
      361 gnnnnsnsnn nnggnngsnn snndstnshg nqiggtatsp lygsmtrlmy ispltppisd
      421 pgspigappr rtppppypqp simnpshriq pspistkfnr rnnpelekrr vhhcdfigct
      481 kvytksshlk ahqrihtgek pyqcqwpece wrfarsdelt rhyrkhtgak pfkcavcers
      541 farsdhlalh mkrhlpkqha k
//
LOCUS       XP_034179084             560 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603726 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179084
VERSION     XP_034179084.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179084.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..560
                     /product="uncharacterized protein LOC117603726 isoform X2"
                     /calculated_mol_wt=61185
     Region          454..>534
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          478..495
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(480,482,484,486..487,490..491,494,510,512,516..517,
                     520..521,524,538,540,542,544..545,548..549,552)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          503..525
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          531..553
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          533..553
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..560
                     /gene="LOC117603726"
                     /coded_by="XM_034323193.2:2318..4000"
                     /db_xref="GeneID:117603726"
ORIGIN      
        1 mvprgltvgr siweqavlea gpevgpepeq qrfydspppr mdvsvcclpa sagsgaqhph
       61 paslpsggqp tiqppyqyad qivpdrgqpd glpvlgctgq dnwqqisatn vagavtaatt
      121 tttyidyswl qmqtseldtl lcrqdldrlq kldeddpekd sressvqmed ffevggpvcr
      181 pqasfvssrs qpagndddvf lrpplwedit ssiqkldpen admlasqshv kmetedvasp
      241 vlspveikte plpppptlhl legptsnsvq tyvnghapas tdpqvvhhrt sttayktfss
      301 nnnnnnnnnn nannnnnnnt ttnnnntvsv nnngngqghg hghghghghg hgnnnnvgng
      361 nnnnsnsnnn nggnngsnns nndstnshgn qiggtatspl ygsmtrlmyi spltppisdp
      421 gspigapprr tppppypqps imnpshriqp spistkfnrr nnpelekrrv hhcdfigctk
      481 vytksshlka hqrihtgekp yqcqwpecew rfarsdeltr hyrkhtgakp fkcavcersf
      541 arsdhlalhm krhlpkqhak
//
LOCUS       XP_034179092             533 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603726 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179092
VERSION     XP_034179092.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323201.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179092.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="uncharacterized protein LOC117603726 isoform X3"
                     /calculated_mol_wt=57956
     Region          <445..469
                     /region_name="KLF5_N"
                     /note="N-terminal domain of Kruppel-like factor 5;
                     cd21579"
                     /db_xref="CDD:410335"
     CDS             1..533
                     /gene="LOC117603726"
                     /coded_by="XM_034323201.2:2318..3919"
                     /db_xref="GeneID:117603726"
ORIGIN      
        1 mvprgltvgr siweqavlea gpevgpepeq qrfydspppr mdvsvcclpa sagsgaqhph
       61 paslpsggqp tiqppyqyad qivpdrgqpd glpvlgctgq dnwqqisatn vagavtaatt
      121 tttyidyswl qmqqtseldt llcrqdldrl qkldeddpek dsressvqme dffevggpvc
      181 rpqasfvssr sqpagndddv flrpplwedi tssiqkldpe nadmlasqsh vkmetedvas
      241 pvlspveikt eplpppptlh llegptsnsv qtyvnghapa stdpqvvhhr tsttayktfs
      301 snnnnnnnnn nnannnnnnn tttnnnntvs vnnngngqgh ghghghghgh ghgnnnnvgn
      361 gnnnnsnsnn nnggnngsnn snndstnshg nqiggtatsp lygsmtrlmy ispltppisd
      421 pgspigappr rtppppypqp simnpshriq pspistkfnr rnnpelekrr vhhcdfivlf
      481 ciqtlpekqq qqqqqrhrfe eqltvsviis fsrlykglhe efalegaptd thr
//
LOCUS       XP_034179098             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603726 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179098
VERSION     XP_034179098.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323207.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179098.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117603726 isoform X4"
                     /calculated_mol_wt=52873
     Region          <445..469
                     /region_name="KLF5_N"
                     /note="N-terminal domain of Kruppel-like factor 5;
                     cd21579"
                     /db_xref="CDD:410335"
     CDS             1..493
                     /gene="LOC117603726"
                     /coded_by="XM_034323207.2:2318..3799"
                     /db_xref="GeneID:117603726"
ORIGIN      
        1 mvprgltvgr siweqavlea gpevgpepeq qrfydspppr mdvsvcclpa sagsgaqhph
       61 paslpsggqp tiqppyqyad qivpdrgqpd glpvlgctgq dnwqqisatn vagavtaatt
      121 tttyidyswl qmqqtseldt llcrqdldrl qkldeddpek dsressvqme dffevggpvc
      181 rpqasfvssr sqpagndddv flrpplwedi tssiqkldpe nadmlasqsh vkmetedvas
      241 pvlspveikt eplpppptlh llegptsnsv qtyvnghapa stdpqvvhhr tsttayktfs
      301 snnnnnnnnn nnannnnnnn tttnnnntvs vnnngngqgh ghghghghgh ghgnnnnvgn
      361 gnnnnsnsnn nnggnngsnn snndstnshg nqiggtatsp lygsmtrlmy ispltppisd
      421 pgspigappr rtppppypqp simnpshriq pspistkfnr rnnpelekrr vhhcdfidas
      481 geattttttt tsi
//
LOCUS       XP_034179111             495 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603759 [Osmia lignaria lignaria].
ACCESSION   XP_034179111
VERSION     XP_034179111.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323220.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179111.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="uncharacterized protein LOC117603759"
                     /calculated_mol_wt=55881
     Region          <224..431
                     /region_name="Glycosyltransferase_GTB-type"
                     /note="glycosyltransferase family 1 and related proteins
                     with GTB topology; cl10013"
                     /db_xref="CDD:471961"
     CDS             1..495
                     /gene="LOC117603759"
                     /coded_by="XM_034323220.2:1033..2520"
                     /db_xref="GeneID:117603759"
ORIGIN      
        1 mlplfalcmc lylvnnagts tlttppqsav vvafedvydl sllantlsdq gidatliipd
       61 syenelyetl idvevltvkl kteestspek ravqaceall tdegiakkiq eiqptftifp
      121 alrhdgcllp waktiesipa vwtrnrdeel yvfeytnaaw pvqsaglwtr lwtsfsrrti
      181 fsttrdeyva yalriagkyl pdtglnldnl ytdlrlilwg sdvvlrsdfv pltqlivevg
      241 chhcrgahpl qgdlhkslle frlgtvvvll denyhtlike laeklpqgrd gqalvwknmk
      301 wqssssampd nlfiqsgidr qdligysrtr vmlshcadse lleaafhgtp vicfprnsqe
      361 sqnaaravql gfarstedih avsseeiant vnqihetmdy renarkvsla irdrinpavd
      421 rliywlryma rtrdwnidfl aadpsvktfn edlqfffglf vgililgiss tvgclltryl
      481 vvskriqrsk gryar
//
LOCUS       XP_034179118             495 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603759 [Osmia lignaria lignaria].
ACCESSION   XP_034179118
VERSION     XP_034179118.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323227.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179118.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="uncharacterized protein LOC117603759"
                     /calculated_mol_wt=55881
     Region          <224..431
                     /region_name="Glycosyltransferase_GTB-type"
                     /note="glycosyltransferase family 1 and related proteins
                     with GTB topology; cl10013"
                     /db_xref="CDD:471961"
     CDS             1..495
                     /gene="LOC117603759"
                     /coded_by="XM_034323227.2:957..2444"
                     /db_xref="GeneID:117603759"
ORIGIN      
        1 mlplfalcmc lylvnnagts tlttppqsav vvafedvydl sllantlsdq gidatliipd
       61 syenelyetl idvevltvkl kteestspek ravqaceall tdegiakkiq eiqptftifp
      121 alrhdgcllp waktiesipa vwtrnrdeel yvfeytnaaw pvqsaglwtr lwtsfsrrti
      181 fsttrdeyva yalriagkyl pdtglnldnl ytdlrlilwg sdvvlrsdfv pltqlivevg
      241 chhcrgahpl qgdlhkslle frlgtvvvll denyhtlike laeklpqgrd gqalvwknmk
      301 wqssssampd nlfiqsgidr qdligysrtr vmlshcadse lleaafhgtp vicfprnsqe
      361 sqnaaravql gfarstedih avsseeiant vnqihetmdy renarkvsla irdrinpavd
      421 rliywlryma rtrdwnidfl aadpsvktfn edlqfffglf vgililgiss tvgclltryl
      481 vvskriqrsk gryar
//
LOCUS       XP_076546145             472 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial DNA helicase [Osmia lignaria lignaria].
ACCESSION   XP_076546145
VERSION     XP_076546145.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690030.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..472
                     /product="mitochondrial DNA helicase"
                     /calculated_mol_wt=54438
     Region          338..>471
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     CDS             1..472
                     /gene="mtDNA-helicase"
                     /coded_by="XM_076690030.1:612..2030"
                     /db_xref="GeneID:117603682"
ORIGIN      
        1 mfiklflrtc iqkqkqhirs sirkqcihtn tkestmnisi shlkysfkqq clnvtegytc
       61 iiadcpicnd tkkgskiyvn kttgffmcdq ckftgswnil ekflsmkksn dklkelemlr
      121 atchleenfe eewnekirnc qnvadmsiee ynmllnnfsl pyvlqedisq lncfyekvtn
      181 viyfpligln nsivgykvlt teagieqtvp algvggciky kkynsksdnv avvvgmiddl
      241 laliskkltn siiclpynlk slpqqllpcm esfkklilwf gndetswyta rhfakklndk
      301 rcyfirptds qprpklaaei gydfksilqn aqpvwhksii tfhhlreevl sdlqnidkvq
      361 gvkwkkyptl nrilkghrrg eftvltgptg cgkttfmsey sldlamqdvs tlwgsfeirn
      421 vrlaktmlqq mvgvpldqnl enfnvyadaf eklpiyfmtf hgqqnikvvm dq
//
LOCUS       XP_034172075             465 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 36 member pathetic [Osmia lignaria lignaria].
ACCESSION   XP_034172075
VERSION     XP_034172075.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316184.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..465
                     /product="solute carrier family 36 member pathetic"
                     /calculated_mol_wt=51160
     Region          63..453
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..465
                     /gene="path"
                     /coded_by="XM_034316184.2:323..1720"
                     /db_xref="GeneID:117600586"
ORIGIN      
        1 mptemhtflp qdgsnpkdgv ltkykvqvap rdpeaaqgdg ktydpfteri vdnpttdcdt
       61 lthllkaslg tgilsmpiaf knaglllgvf stilvafvct hcayilvkca hvlyyktkrs
      121 emsfadvaea afatgpqwgr kfarpiryli qislfatyfg tcsvytviva anfkqiiqyy
      181 kdedspefsl rliaacllip millsyipnl kylapvsmva nifmgtglgi tfyylvwdlp
      241 pinsvplfas iedfprffsi tifameaigv vmplennmkt pqhfvgicgv lnkgmsgvtl
      301 iyillgflgy vkyqdltsds itlnlptaei paqvvkilia lavyctfglq fyvcldiawn
      361 gikdrfqkkp llanyilrtv mvtgavllav ivptiepfig ligafcfsil gllipvfvet
      421 vtywdvgfga gnwvalknii iciigmmali fgsrsaliqi valys
//
LOCUS       XP_034179129             460 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoporin 62 [Osmia lignaria lignaria].
ACCESSION   XP_034179129
VERSION     XP_034179129.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323238.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="nucleoporin 62"
                     /calculated_mol_wt=47775
     Region          28..>280
                     /region_name="Nucleoporin_FG2"
                     /note="Nucleoporin FG repeated region; pfam15967"
                     /db_xref="CDD:435043"
     Region          <238..>452
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     Region          263..362
                     /region_name="Nsp1_C"
                     /note="Nsp1-like C-terminal region; pfam05064"
                     /db_xref="CDD:461540"
     CDS             1..460
                     /gene="Nup62"
                     /coded_by="XM_034323238.2:230..1612"
                     /db_xref="GeneID:117603766"
ORIGIN      
        1 msftlnpakp qatatpatgt ptttfafgat gagdaakptg fsltptpaqn kpggvfgtgl
       61 tntppqntgf nlgapigtpg stlplqtpgl gfgistpast qgatpgvvts aipvgfpaaa
      121 pagntlgfnl ggtttatttt atttsafask pltsgfglga geavsstttt gltfgtpntm
      181 avgtgfaipa staattasal ttapatgfsl ntstttsapt gfafgtstaa atttgfslnk
      241 tsaapsfttp ssqvslgttt tvsssantfq pasissfees inkwtlelee qekvfvnqaa
      301 qvnawdklli sngekivtln qevervkieq qqleheldyv vgqqkelqdc lvplekelas
      361 lsvsdperey tyrlaedldt qlkrmsedlk eiiehlnqan rtqdssdpiv qigkilnahm
      421 nslqwidqqt sllnqkiqqi dqmhqnfrqe nersfnlayn
//
LOCUS       XP_034179135             460 aa            linear   INV 03-SEP-2025
DEFINITION  nucleoporin 62 [Osmia lignaria lignaria].
ACCESSION   XP_034179135
VERSION     XP_034179135.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323244.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="nucleoporin 62"
                     /calculated_mol_wt=47775
     Region          28..>280
                     /region_name="Nucleoporin_FG2"
                     /note="Nucleoporin FG repeated region; pfam15967"
                     /db_xref="CDD:435043"
     Region          <238..>452
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     Region          263..362
                     /region_name="Nsp1_C"
                     /note="Nsp1-like C-terminal region; pfam05064"
                     /db_xref="CDD:461540"
     CDS             1..460
                     /gene="Nup62"
                     /coded_by="XM_034323244.2:239..1621"
                     /db_xref="GeneID:117603766"
ORIGIN      
        1 msftlnpakp qatatpatgt ptttfafgat gagdaakptg fsltptpaqn kpggvfgtgl
       61 tntppqntgf nlgapigtpg stlplqtpgl gfgistpast qgatpgvvts aipvgfpaaa
      121 pagntlgfnl ggtttatttt atttsafask pltsgfglga geavsstttt gltfgtpntm
      181 avgtgfaipa staattasal ttapatgfsl ntstttsapt gfafgtstaa atttgfslnk
      241 tsaapsfttp ssqvslgttt tvsssantfq pasissfees inkwtlelee qekvfvnqaa
      301 qvnawdklli sngekivtln qevervkieq qqleheldyv vgqqkelqdc lvplekelas
      361 lsvsdperey tyrlaedldt qlkrmsedlk eiiehlnqan rtqdssdpiv qigkilnahm
      421 nslqwidqqt sllnqkiqqi dqmhqnfrqe nersfnlayn
//
LOCUS       XP_076546146             455 aa            linear   INV 03-SEP-2025
DEFINITION  immunoglobulin domain-containing protein Bsg isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546146
VERSION     XP_076546146.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690031.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..455
                     /product="immunoglobulin domain-containing protein Bsg
                     isoform X1"
                     /calculated_mol_wt=50699
     Region          25..100
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          <126..188
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          204..298
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          215..219
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          228..232
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          254..262
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          273..278
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          288..294
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..455
                     /gene="Bsg"
                     /coded_by="XM_076690031.1:651..2018"
                     /db_xref="GeneID:117603789"
ORIGIN      
        1 merraafvll gmlylagtlv tvqavtykgl asivhegqpw wieckdlkpd dlirwtrngq
       61 slepelasgq lvvhspvgtg sstltamhat dshdgdykct pdssevfhlw iyvdikikvl
      121 tpkdapmtle cgnrtagekf qwfkenvplh tavadseelf eldndngtle llkstevlyg
      181 nysckgsnss teyrivskpt ahlpdstsvv egeklhlvca gkqspgikvs wtfgdqnytr
      241 skgrvklgrd qergiygavl iveniemndr gsvycrvsyn wsdtaedhia eaqtflrvkd
      301 klaalwpflg icaevvvlca iiliyekkrn kaeleesdtd qspdtepstk ddrleyyipl
      361 rgpippsdlp vdpppvvtpp tpetvdegsd rlnssspast pqrilsapaq ltlsryrfat
      421 fpliscrrfw nyaqreyfrs ketraepass lqdic
//
LOCUS       XP_076546147             366 aa            linear   INV 03-SEP-2025
DEFINITION  immunoglobulin domain-containing protein Bsg isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546147
VERSION     XP_076546147.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690032.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="immunoglobulin domain-containing protein Bsg
                     isoform X2"
                     /calculated_mol_wt=40807
     Region          <37..99
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          115..209
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          126..130
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          139..143
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          165..173
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          184..189
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..205
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..366
                     /gene="Bsg"
                     /coded_by="XM_076690032.1:654..1754"
                     /db_xref="GeneID:117603789"
ORIGIN      
        1 merraafvll gmlylagtlv tvqadikikv ltpkdapmtl ecgnrtagek fqwfkenvpl
       61 htavadseel feldndngtl ellkstevly gnysckgsns steyrivskp tahlpdstsv
      121 vegeklhlvc agkqspgikv swtfgdqnyt rskgrvklgr dqergiygav liveniemnd
      181 rgsvycrvsy nwsdtaedhi aeaqtflrvk dklaalwpfl gicaevvvlc aiiliyekkr
      241 nkaeleesdt dqspdtepst kddrleyyip lrgpippsdl pvdpppvvtp ptpetvdegs
      301 drlnssspas tpqrilsapa qltlsryrfa tfpliscrrf wnyaqreyfr sketraepas
      361 slqdic
//
LOCUS       XP_034179164             359 aa            linear   INV 03-SEP-2025
DEFINITION  immunoglobulin domain-containing protein Bsg isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034179164
VERSION     XP_034179164.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323273.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..359
                     /product="immunoglobulin domain-containing protein Bsg
                     isoform X3"
                     /calculated_mol_wt=39944
     Region          25..100
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          <126..188
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          204..298
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          215..219
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          228..232
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          254..262
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          273..278
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          288..294
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..359
                     /gene="Bsg"
                     /coded_by="XM_034323273.2:645..1724"
                     /db_xref="GeneID:117603789"
ORIGIN      
        1 merraafvll gmlylagtlv tvqavtykgl asivhegqpw wieckdlkpd dlirwtrngq
       61 slepelasgq lvvhspvgtg sstltamhat dshdgdykct pdssevfhlw iyvdikikvl
      121 tpkdapmtle cgnrtagekf qwfkenvplh tavadseelf eldndngtle llkstevlyg
      181 nysckgsnss teyrivskpt ahlpdstsvv egeklhlvca gkqspgikvs wtfgdqnytr
      241 skgrvklgrd qergiygavl iveniemndr gsvycrvsyn wsdtaedhia eaqtflrvkd
      301 klaalwpflg icaevvvlca iiliyekkrn kaeleesdtd qspdtkptpn kdsdvrqrk
//
LOCUS       XP_034176719             438 aa            linear   INV 03-SEP-2025
DEFINITION  glutathione S-transferase LANCL1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176719
VERSION     XP_034176719.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320828.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..438
                     /product="glutathione S-transferase LANCL1 isoform X1"
                     /calculated_mol_wt=50071
     Region          97..434
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..438
                     /gene="LOC117602580"
                     /coded_by="XM_034320828.2:79..1395"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mfqaiiryki kaeqtstkrl ntikqfrrlf cdkmddnrhy nnpfedypnp sansivnast
       61 nevnenyket lrmhtkrlld elkekrhnwt ynddcsvytg tagiaymyyq ygkcfnqpay
      121 ieqatewlri cvskfrgrre vtfltgsvgp qalsavvlhs qghkeealnm iskvksvsay
      181 vldecsdlpd ellygragyl fcllflnsni spppiedeli kqvialiiks gnvysasrky
      241 ktplmyawhd seylggahgl ggilymllqa rqyltqsqlt rdikpaldfl qslrypsgnc
      301 pssigsrtdk lvhwchgapg mimlftlaye iyqdeqylnt alqcgevvwa rgllkkgcgi
      361 chgaagnayt fltlfqktkd vkhlyracrf aewctdykah qtripdrpys lfeglagtiy
      421 flidiqhphl akfpayti
//
LOCUS       XP_034176792             405 aa            linear   INV 03-SEP-2025
DEFINITION  lanC-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034176792
VERSION     XP_034176792.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320901.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="lanC-like protein 2 isoform X2"
                     /calculated_mol_wt=46012
     Region          64..401
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..405
                     /gene="LOC117602580"
                     /coded_by="XM_034320901.2:744..1961"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mddnrhynnp fedypnpsan sivnastnev nenyketlrm htkrlldelk ekrhnwtynd
       61 dcsvytgtag iaymyyqygk cfnqpayieq atewlricvs kfrgrrevtf ltgsvgpqal
      121 savvlhsqgh keealnmisk vksvsayvld ecsdlpdell ygragylfcl lflnsnispp
      181 piedelikqv ialiiksgnv ysasrkyktp lmyawhdsey lggahglggi lymllqarqy
      241 ltqsqltrdi kpaldflqsl rypsgncpss igsrtdklvh wchgapgmim lftlayeiyq
      301 deqylntalq cgevvwargl lkkgcgichg aagnaytflt lfqktkdvkh lyracrfaew
      361 ctdykahqtr ipdrpyslfe glagtiyfli diqhphlakf payti
//
LOCUS       XP_076546148             405 aa            linear   INV 03-SEP-2025
DEFINITION  lanC-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546148
VERSION     XP_076546148.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690033.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="lanC-like protein 2 isoform X2"
                     /calculated_mol_wt=46012
     Region          64..401
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..405
                     /gene="LOC117602580"
                     /coded_by="XM_076690033.1:356..1573"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mddnrhynnp fedypnpsan sivnastnev nenyketlrm htkrlldelk ekrhnwtynd
       61 dcsvytgtag iaymyyqygk cfnqpayieq atewlricvs kfrgrrevtf ltgsvgpqal
      121 savvlhsqgh keealnmisk vksvsayvld ecsdlpdell ygragylfcl lflnsnispp
      181 piedelikqv ialiiksgnv ysasrkyktp lmyawhdsey lggahglggi lymllqarqy
      241 ltqsqltrdi kpaldflqsl rypsgncpss igsrtdklvh wchgapgmim lftlayeiyq
      301 deqylntalq cgevvwargl lkkgcgichg aagnaytflt lfqktkdvkh lyracrfaew
      361 ctdykahqtr ipdrpyslfe glagtiyfli diqhphlakf payti
//
LOCUS       XP_076546149             405 aa            linear   INV 03-SEP-2025
DEFINITION  lanC-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546149
VERSION     XP_076546149.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690034.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="lanC-like protein 2 isoform X2"
                     /calculated_mol_wt=46012
     Region          64..401
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..405
                     /gene="LOC117602580"
                     /coded_by="XM_076690034.1:251..1468"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mddnrhynnp fedypnpsan sivnastnev nenyketlrm htkrlldelk ekrhnwtynd
       61 dcsvytgtag iaymyyqygk cfnqpayieq atewlricvs kfrgrrevtf ltgsvgpqal
      121 savvlhsqgh keealnmisk vksvsayvld ecsdlpdell ygragylfcl lflnsnispp
      181 piedelikqv ialiiksgnv ysasrkyktp lmyawhdsey lggahglggi lymllqarqy
      241 ltqsqltrdi kpaldflqsl rypsgncpss igsrtdklvh wchgapgmim lftlayeiyq
      301 deqylntalq cgevvwargl lkkgcgichg aagnaytflt lfqktkdvkh lyracrfaew
      361 ctdykahqtr ipdrpyslfe glagtiyfli diqhphlakf payti
//
LOCUS       XP_034176882             405 aa            linear   INV 03-SEP-2025
DEFINITION  lanC-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034176882
VERSION     XP_034176882.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320991.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="lanC-like protein 2 isoform X2"
                     /calculated_mol_wt=46012
     Region          64..401
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..405
                     /gene="LOC117602580"
                     /coded_by="XM_034320991.2:720..1937"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mddnrhynnp fedypnpsan sivnastnev nenyketlrm htkrlldelk ekrhnwtynd
       61 dcsvytgtag iaymyyqygk cfnqpayieq atewlricvs kfrgrrevtf ltgsvgpqal
      121 savvlhsqgh keealnmisk vksvsayvld ecsdlpdell ygragylfcl lflnsnispp
      181 piedelikqv ialiiksgnv ysasrkyktp lmyawhdsey lggahglggi lymllqarqy
      241 ltqsqltrdi kpaldflqsl rypsgncpss igsrtdklvh wchgapgmim lftlayeiyq
      301 deqylntalq cgevvwargl lkkgcgichg aagnaytflt lfqktkdvkh lyracrfaew
      361 ctdykahqtr ipdrpyslfe glagtiyfli diqhphlakf payti
//
LOCUS       XP_076546150             405 aa            linear   INV 03-SEP-2025
DEFINITION  lanC-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546150
VERSION     XP_076546150.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690035.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="lanC-like protein 2 isoform X2"
                     /calculated_mol_wt=46012
     Region          64..401
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..405
                     /gene="LOC117602580"
                     /coded_by="XM_076690035.1:332..1549"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mddnrhynnp fedypnpsan sivnastnev nenyketlrm htkrlldelk ekrhnwtynd
       61 dcsvytgtag iaymyyqygk cfnqpayieq atewlricvs kfrgrrevtf ltgsvgpqal
      121 savvlhsqgh keealnmisk vksvsayvld ecsdlpdell ygragylfcl lflnsnispp
      181 piedelikqv ialiiksgnv ysasrkyktp lmyawhdsey lggahglggi lymllqarqy
      241 ltqsqltrdi kpaldflqsl rypsgncpss igsrtdklvh wchgapgmim lftlayeiyq
      301 deqylntalq cgevvwargl lkkgcgichg aagnaytflt lfqktkdvkh lyracrfaew
      361 ctdykahqtr ipdrpyslfe glagtiyfli diqhphlakf payti
//
LOCUS       XP_076546151             405 aa            linear   INV 03-SEP-2025
DEFINITION  lanC-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546151
VERSION     XP_076546151.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690036.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="lanC-like protein 2 isoform X2"
                     /calculated_mol_wt=46012
     Region          64..401
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..405
                     /gene="LOC117602580"
                     /coded_by="XM_076690036.1:433..1650"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mddnrhynnp fedypnpsan sivnastnev nenyketlrm htkrlldelk ekrhnwtynd
       61 dcsvytgtag iaymyyqygk cfnqpayieq atewlricvs kfrgrrevtf ltgsvgpqal
      121 savvlhsqgh keealnmisk vksvsayvld ecsdlpdell ygragylfcl lflnsnispp
      181 piedelikqv ialiiksgnv ysasrkyktp lmyawhdsey lggahglggi lymllqarqy
      241 ltqsqltrdi kpaldflqsl rypsgncpss igsrtdklvh wchgapgmim lftlayeiyq
      301 deqylntalq cgevvwargl lkkgcgichg aagnaytflt lfqktkdvkh lyracrfaew
      361 ctdykahqtr ipdrpyslfe glagtiyfli diqhphlakf payti
//
LOCUS       XP_034176968             366 aa            linear   INV 03-SEP-2025
DEFINITION  lanC-like protein 2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034176968
VERSION     XP_034176968.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321077.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="lanC-like protein 2 isoform X3"
                     /calculated_mol_wt=41456
     Region          25..362
                     /region_name="euk_LANCL"
                     /note="Eukaryotic Lanthionine synthetase C-like protein;
                     cd04794"
                     /db_xref="CDD:271202"
     CDS             1..366
                     /gene="LOC117602580"
                     /coded_by="XM_034321077.2:152..1252"
                     /db_xref="GeneID:117602580"
ORIGIN      
        1 mhtkrlldel kekrhnwtyn ddcsvytgta giaymyyqyg kcfnqpayie qatewlricv
       61 skfrgrrevt fltgsvgpqa lsavvlhsqg hkeealnmis kvksvsayvl decsdlpdel
      121 lygragylfc llflnsnisp ppiedelikq vialiiksgn vysasrkykt plmyawhdse
      181 ylggahglgg ilymllqarq yltqsqltrd ikpaldflqs lrypsgncps sigsrtdklv
      241 hwchgapgmi mlftlayeiy qdeqylntal qcgevvwarg llkkgcgich gaagnaytfl
      301 tlfqktkdvk hlyracrfae wctdykahqt ripdrpyslf eglagtiyfl idiqhphlak
      361 fpayti
//
LOCUS       XP_034172090             427 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600591 [Osmia lignaria lignaria].
ACCESSION   XP_034172090
VERSION     XP_034172090.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316199.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="uncharacterized protein LOC117600591"
                     /calculated_mol_wt=47428
     Region          103..208
                     /region_name="DUF4497"
                     /note="Protein of unknown function (DUF4497); pfam14924"
                     /db_xref="CDD:464380"
     CDS             1..427
                     /gene="LOC117600591"
                     /coded_by="XM_034316199.2:243..1526"
                     /db_xref="GeneID:117600591"
ORIGIN      
        1 mdrghrskkk slkddkikkd ssltlgsrdq avpeakehyl flleffihql tserlaklnq
       61 mffvpttisv eflnfknedg ieitpvdpmf qpqagvaddv eyfysgrsvl faidqytvvn
      121 kmlelvidlc vrksmpegvk pdvlvgkgel dmskhfaalr kemlqcwhkm vppprvfege
      181 vplmyneeei gaacmfvris afgqtivtef dappertvss yvfkgdasdr kspvykcriv
      241 dsqiadvcrd skdelyaggp pcrvcvkeqh pctpcgdpcg atlkpnvsps rqfeseprsr
      301 pvsgtrskad ckqagliqss rgpaqpcgka vvlkvsgild tgkdkkptvt vapeceatdp
      361 hsvdpdhdif vlrigkkgiv gageksdiql emktpkgper gppiryetre mqtdeskgkk
      421 kkkkkkn
//
LOCUS       XP_034179148             414 aa            linear   INV 03-SEP-2025
DEFINITION  protein Wnt-4 [Osmia lignaria lignaria].
ACCESSION   XP_034179148
VERSION     XP_034179148.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323257.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="protein Wnt-4"
                     /calculated_mol_wt=45675
     Region          101..414
                     /region_name="wnt"
                     /note="wnt family; pfam00110"
                     /db_xref="CDD:459677"
     CDS             1..414
                     /gene="LOC117603780"
                     /coded_by="XM_034323257.2:119..1363"
                     /db_xref="GeneID:117603780"
ORIGIN      
        1 mdrsltkrcl svvvrsdcit klqvqriltk mrpllttffv vpfllvawqn gaqanwwylg
       61 vesrlnsgsd qgsgadgqaq ivgagvigpa saekncksmh ltskqqaics rsppvlqavs
      121 agarlaieec qhqfrsarwn csispenpdn vfggvmlvns reaafvyais aagvaysvtr
      181 acsrgeltdc scdnrvrtrr pnnwqwggcs edihfgekfs rewsdggeep vkedvlqgpk
      241 glagqlmrkh dseagrravr srmqrvckch gmsgscsvkv cwrklpafrv agaalaalhe
      301 gaalvrlaqr ggrrparlrp arpdlkrpnk tdlvyledsp dyceknialg ipgtrgricn
      361 rtslgldgcr llccgrgyqt rvrdvtekcn crfvwccnvk celcrhkree hvcn
//
LOCUS       XP_034172125             361 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600606 [Osmia lignaria lignaria].
ACCESSION   XP_034172125
VERSION     XP_034172125.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316234.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172125.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..361
                     /product="uncharacterized protein LOC117600606"
                     /calculated_mol_wt=38286
     Region          65..171
                     /region_name="DUF4497"
                     /note="Protein of unknown function (DUF4497); pfam14924"
                     /db_xref="CDD:464380"
     CDS             1..361
                     /gene="LOC117600606"
                     /coded_by="XM_034316234.2:147..1232"
                     /db_xref="GeneID:117600606"
ORIGIN      
        1 mgeeqqlflv eflvekvkip viramqdell pactcvsfqi lglppvnicq esltdgcgce
       61 dgdtqifkkg ksclfalpsi vlqrpvtsfp vklsvykklp pgvlpdvmlv gchqieakal
      121 inavlsqkvf kianpcqtik dtfkittatg qcvgavtvyi rvscfgnkii tqfqvphnrk
      181 pylfkgadds pvfqckkips scdtpapvkc tctkkcidgs gesagrtccq tplvvaspcp
      241 plpdkqpdwg prpccasrqs ppggqggscp csskspqkyq dtdptvkfgg csavhqqpad
      301 gkckpccgsa qpvqkdpcpp ccgasssgsk gykgfsdlas ketptkkcgc pvkdyscncg
      361 k
//
LOCUS       XP_034179178             334 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic iron-sulfur assembly component 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034179178
VERSION     XP_034179178.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323287.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179178.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..334
                     /product="cytosolic iron-sulfur assembly component 1
                     isoform X1"
                     /calculated_mol_wt=37408
     Region          6..330
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(13,31,35,41,43,57..58,76,80,86..87,101..102,119,124,
                     130..131,146,163,168,174..175,191..192,210,214,220,222,
                     248,250,267,272,278,281,300..301,320,324,330)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          17..57
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          63..101
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          106..144
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          151..190
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          196..247
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          254..299
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          305..330
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..334
                     /gene="Ciao1"
                     /coded_by="XM_034323287.2:183..1187"
                     /db_xref="GeneID:117603796"
ORIGIN      
        1 mgtlelkqtl nghrgrvwnv cwhpkgiyla scgedktiii wgpqepkwai ktilteghsr
       61 tirevawspc gnyiasasfd attavwdkks gqfecnatle ghenevksvs wstsgqllat
      121 csrdksvwiw evnddeyece avisahtqdv kkvrwhptee ivasasydnt vkifkeeaaa
      181 ndwsclatls shtstvwsls wnkegnriat csddqtvkiw qefkpgneqg iatannepvw
      241 kcvctisgyh trtiydidwc kttdlivtac gddiirifke dsdsdslqpt ftmvcsmdta
      301 htqdvncvqw nptvpgqlas csddglvkiw fyne
//
LOCUS       XP_034179196             303 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic iron-sulfur assembly component 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034179196
VERSION     XP_034179196.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323305.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="cytosolic iron-sulfur assembly component 1
                     isoform X2"
                     /calculated_mol_wt=33365
     Region          44..277
                     /region_name="NIF3"
                     /note="NIF3 (NGG1p interacting factor 3); pfam01784"
                     /db_xref="CDD:426431"
     CDS             1..303
                     /gene="Ciao1"
                     /coded_by="XM_034323305.2:177..1088"
                     /db_xref="GeneID:117603796"
ORIGIN      
        1 mfrhftalkr scrifvydtk qkhssvlyam samkpdamgi pltqvvetlh kfadpslanp
       61 wdnvglliep tetklvshil ltndltesvm qealdlktdm iityhplifa pmksvttrsw
      121 kekivakcle nriavyspht sfdsvkggvn dwlasafedv lksshpievn vsnpdygcgr
      181 icilkskisi edavqrvkeh tglkyvrlar acrtdgfint ialcagsgas vlkgvsadly
      241 ltgemlhhdi ldathngisv iltnhsdser gfltkfaftl sdvldksvnv svskndadpl
      301 ktv
//
LOCUS       XP_034188214             326 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit I [Osmia
            lignaria lignaria].
ACCESSION   XP_034188214
VERSION     XP_034188214.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332323.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="eukaryotic translation initiation factor 3
                     subunit I"
                     /calculated_mol_wt=36469
     Region          <6..312
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          13..49
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          55..92
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          97..145
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          150..186
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          193..226
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          233..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..326
                     /gene="eIF3i"
                     /coded_by="XM_034332323.2:163..1143"
                     /db_xref="GeneID:117607960"
ORIGIN      
        1 mkplmlhghe raitkikynr egdllfsssk dkkpnvwysl ngerlgsfng hngsvwcidv
       61 nwdttrflsg sgdnslrvwd cetgkeigll etnssvrtcs fsysanlavy stdkalghqc
      121 emfiidirnv daklsqedai sriavtgpri sailwgalde tiitghedge ialwdvrtrk
      181 klssvkghks qindmqfnkd gtmfvtaskd ntaklfdsds lmllktykte rpvnsatisp
      241 vydhvvlggg qdamdvttts trqgkfdsrf fhlvfeeefa rlkghfgpin slafhpngrs
      301 istggedgyv rintfdqsyf dfhfey
//
LOCUS       XP_034179209             320 aa            linear   INV 03-SEP-2025
DEFINITION  CDK-activating kinase assembly factor [Osmia lignaria lignaria].
ACCESSION   XP_034179209
VERSION     XP_034179209.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323318.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="CDK-activating kinase assembly factor"
                     /calculated_mol_wt=36713
     Region          1..311
                     /region_name="cdk7"
                     /note="CDK-activating kinase assembly factor MAT1;
                     TIGR00570"
                     /db_xref="CDD:129661"
     CDS             1..320
                     /gene="Mat1"
                     /coded_by="XM_034323318.2:310..1272"
                     /db_xref="GeneID:117603811"
ORIGIN      
        1 medqacprck ttkyrnpslk mmvnvcghtl cescvdllfl kgsgscpeck iplrranfri
       61 qlfedpmvek dinirkkvlk dfnkkeedfa tlreyndyle evetiiynlv nnidvvetnk
      121 kieqykkdnk eqitknkskl srseyeleem ielekqkeee rrleivkeem eakkkkvrek
      181 ealideltfs egdaknivet hvsamqafkk evkavlpkat qfstgikfgs qgsqnympmp
      241 kieegplysy npiqdttdgp ippgwrelqa rgyvsnvrge ssseraggfk ahvaclralq
      301 eamaglyynp sqcqteftnv
//
LOCUS       XP_034179221             315 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor IIB [Osmia lignaria lignaria].
ACCESSION   XP_034179221
VERSION     XP_034179221.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323330.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="transcription factor IIB"
                     /calculated_mol_wt=34166
     Region          1..289
                     /region_name="SUA7"
                     /note="Transcription initiation factor TFIIIB, Brf1
                     subunit/Transcription initiation factor TFIIB
                     [Transcription]; COG1405"
                     /db_xref="CDD:441015"
     CDS             1..315
                     /gene="TfIIB"
                     /coded_by="XM_034323330.2:136..1083"
                     /db_xref="GeneID:117603820"
ORIGIN      
        1 masssrhetn kvccyahpda pliedyragd qicsecglvv gdrvidvgse wrtfsnekag
       61 vdpsrvggpe npllngsdls tmigpgtgaa sfdafgvsky qnrrtmsssd ralinafrei
      121 ngmadrinlp ktivdrannl fkqvhdgknl kgrandaias aclyiacrqe gvprtfkeic
      181 avskiskkei grcfklilka letsvdlitt gdfmsrfcsn lglpnmvqra athiarkave
      241 idivpgrspi svaaaaiyma sqasedkrsq keigdiagva dvtirqsykl myphagklfp
      301 edfkfatpid qlpqm
//
LOCUS       XP_034179228             278 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger and CHY zinc finger domain containing 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034179228
VERSION     XP_034179228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323337.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="ring finger and CHY zinc finger domain
                     containing 1 isoform X1"
                     /calculated_mol_wt=31563
     Region          32..105
                     /region_name="zf-CHY"
                     /note="CHY zinc finger; pfam05495"
                     /db_xref="CDD:461665"
     Region          157..201
                     /region_name="RING-H2_Pirh2-like"
                     /note="RING finger, H2 subclass, found in p53-induced
                     RING-H2 protein (Pirh2) and similar proteins; cd16464"
                     /db_xref="CDD:438127"
     Region          205..263
                     /region_name="zinc_ribbon_6"
                     /note="Zinc-ribbon; pfam14599"
                     /db_xref="CDD:464215"
     CDS             1..278
                     /gene="Rchy1"
                     /coded_by="XM_034323337.2:697..1533"
                     /db_xref="GeneID:117603827"
ORIGIN      
        1 menndeartv aerastskee pleatadddy gcehykrksk fvtpccnkvy tcrfchdkee
       61 thtvnrkevt elicvlcdtr qpvqatcqnc hcrfgkytcl ecnlfddedk nqyhcdgcgi
      121 crvggrdrff hcakcnmclp vqlqnghtcv envshancpv cledihtsri pchipncghl
      181 lhrtcfeell hsghyacpic qvslldmtdl wrfldievss tpmpeeyrdy kvdilckdch
      241 eestvkfhvv glkclncgsy ntcrvkgsps padpdtld
//
LOCUS       XP_034179237             277 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger and CHY zinc finger domain containing 1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034179237
VERSION     XP_034179237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323346.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..277
                     /product="ring finger and CHY zinc finger domain
                     containing 1 isoform X2"
                     /calculated_mol_wt=31492
     Region          32..105
                     /region_name="zf-CHY"
                     /note="CHY zinc finger; pfam05495"
                     /db_xref="CDD:461665"
     Region          157..201
                     /region_name="RING-H2_Pirh2-like"
                     /note="RING finger, H2 subclass, found in p53-induced
                     RING-H2 protein (Pirh2) and similar proteins; cd16464"
                     /db_xref="CDD:438127"
     Region          205..263
                     /region_name="zinc_ribbon_6"
                     /note="Zinc-ribbon; pfam14599"
                     /db_xref="CDD:464215"
     CDS             1..277
                     /gene="Rchy1"
                     /coded_by="XM_034323346.2:700..1533"
                     /db_xref="GeneID:117603827"
ORIGIN      
        1 menndeartv aerastskee pleatadddy gcehykrksk fvtpccnkvy tcrfchdkee
       61 thtvnrkevt elicvlcdtr qpvqatcqnc hcrfgkytcl ecnlfddedk nqyhcdgcgi
      121 crvggrdrff hcakcnmclp vqlqnghtcv envshancpv cledihtsri pchipncghl
      181 lhrtcfeell hsghyacpic qvslldmtdl wrfldievss tpmpeeyrdy kvdilckdch
      241 eestvkfhvv glkclncgsy ntcrvkgsps pdpdtld
//
LOCUS       XP_034179246             274 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin [Osmia lignaria lignaria].
ACCESSION   XP_034179246
VERSION     XP_034179246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..274
                     /product="neurotrimin"
                     /calculated_mol_wt=30589
     Region          46..129
                     /region_name="IgV"
                     /note="Immunoglobulin variable domain (IgV); cd00099"
                     /db_xref="CDD:409355"
     Region          49..51
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409355"
     Region          55..63
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409355"
     Region          63..70
                     /region_name="CDR1"
                     /note="CDR1 [structural motif]"
                     /db_xref="CDD:409355"
     Region          71..81
                     /region_name="FR2"
                     /note="FR2 [structural motif]"
                     /db_xref="CDD:409355"
     Region          71..76
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409355"
     Region          79..82
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409355"
     Region          82..95
                     /region_name="CDR2"
                     /note="CDR2 [structural motif]"
                     /db_xref="CDD:409355"
     Region          95..101
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409355"
     Region          96..125
                     /region_name="FR3"
                     /note="FR3 [structural motif]"
                     /db_xref="CDD:409355"
     Region          105..111
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409355"
     Region          119..126
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409355"
     Region          145..227
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..274
                     /gene="LOC117603844"
                     /coded_by="XM_034323355.2:913..1737"
                     /db_xref="GeneID:117603844"
ORIGIN      
        1 mkglfftlsl lfsvqglars tsdqemyenr slepldrgpy fdvsasrnvt alvgktatln
       61 crirnlgdrt vswvrhrdih lltigiqtyt sdqrfvashf prtedwtlqv kypqrrdsgt
      121 yecqvsttpp ighsmhlsvv epvtsivgep emfinkgstm nltcvirhsp eppsaiywth
      181 nhevinydsp rggvsvitek gevttsyllv qraqpadsgh ytchpsnant ktvlvhvlng
      241 ehpaamqhgg qlrlanlpfv misttllafl nhry
//
LOCUS       XP_034179274             273 aa            linear   INV 03-SEP-2025
DEFINITION  hus1-like checkpoint clamp component [Osmia lignaria lignaria].
ACCESSION   XP_034179274
VERSION     XP_034179274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323383.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="hus1-like checkpoint clamp component"
                     /calculated_mol_wt=31474
     Region          1..272
                     /region_name="Hus1"
                     /note="Hus1-like protein; pfam04005"
                     /db_xref="CDD:461127"
     CDS             1..273
                     /gene="Hus1-like"
                     /coded_by="XM_034323383.2:218..1039"
                     /db_xref="GeneID:117603852"
ORIGIN      
        1 mkfkcrmvdv iamrdftnia nifaritkhc tlrltenglc fsvsddrism vwaelsqthf
       61 fteyvmsgvt eeqneiylef dptmlarslg slrmtaksvk ikltnkrqpc ltfeielpsl
      121 siesrqcfhd vpvrviprre waehqtpnip efnisvdmpr lkhvkhiver mknmsptitl
      181 gadksggfvl qvetdsasis thfqnlqvws csqeqddkis vvidvkkfyt flswdivhpd
      241 svkcnmlher mvnlhlhvad ylkiqyfips vat
//
LOCUS       XP_034179286             260 aa            linear   INV 03-SEP-2025
DEFINITION  tafazzin, phospholipid-lysophospholipid transacylase [Osmia
            lignaria lignaria].
ACCESSION   XP_034179286
VERSION     XP_034179286.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323395.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="tafazzin, phospholipid-lysophospholipid
                     transacylase"
                     /calculated_mol_wt=30199
     Region          60..235
                     /region_name="LPLAT_AGPAT-like"
                     /note="Lysophospholipid Acyltransferases (LPLATs) of
                     Glycerophospholipid Biosynthesis: AGPAT-like; cd07989"
                     /db_xref="CDD:153251"
     Site            order(71,74,76,100..103,153..155)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153251"
     CDS             1..260
                     /gene="Taz"
                     /coded_by="XM_034323395.2:244..1026"
                     /db_xref="GeneID:117603860"
ORIGIN      
        1 miydikwiip klrnpsklwn iassitfaav gifskifiew lnkttvynkh vieqalncrp
       61 rnrplitvsn hyscfddpgi watldlryll kprkmrwsla ahdicftktw hsylfmlgkc
      121 ipvirgagvy qeainfciek lavgewihvf pegkvnmfke nmrlkwgvgr lifespvppl
      181 vipiyhlgmd qvlpnevpyr lriknkvtin ygdpidftel lkelrtsktn eiearkaisd
      241 riqtellklk tvteelhett
//
LOCUS       XP_034179299             235 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179299
VERSION     XP_034179299.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323408.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179299.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="uncharacterized protein LOC117603868 isoform X1"
                     /calculated_mol_wt=26548
     CDS             1..235
                     /gene="LOC117603868"
                     /coded_by="XM_034323408.2:249..956"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds nvshfndtsn tfpeeltteq ttdvkqrvty
      181 ddlwaqhrds qmnpkykdfr gsrgmnters avppqresek tappemtyde kniws
//
LOCUS       XP_076546153             235 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546153
VERSION     XP_076546153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690038.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="uncharacterized protein LOC117603868 isoform X1"
                     /calculated_mol_wt=26548
     CDS             1..235
                     /gene="LOC117603868"
                     /coded_by="XM_076690038.1:389..1096"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds nvshfndtsn tfpeeltteq ttdvkqrvty
      181 ddlwaqhrds qmnpkykdfr gsrgmnters avppqresek tappemtyde kniws
//
LOCUS       XP_034179325             235 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179325
VERSION     XP_034179325.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179325.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="uncharacterized protein LOC117603868 isoform X1"
                     /calculated_mol_wt=26548
     CDS             1..235
                     /gene="LOC117603868"
                     /coded_by="XM_034323434.2:225..932"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds nvshfndtsn tfpeeltteq ttdvkqrvty
      181 ddlwaqhrds qmnpkykdfr gsrgmnters avppqresek tappemtyde kniws
//
LOCUS       XP_034179317             235 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179317
VERSION     XP_034179317.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323426.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179317.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="uncharacterized protein LOC117603868 isoform X1"
                     /calculated_mol_wt=26548
     CDS             1..235
                     /gene="LOC117603868"
                     /coded_by="XM_034323426.2:349..1056"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds nvshfndtsn tfpeeltteq ttdvkqrvty
      181 ddlwaqhrds qmnpkykdfr gsrgmnters avppqresek tappemtyde kniws
//
LOCUS       XP_034179335             235 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179335
VERSION     XP_034179335.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323444.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179335.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="uncharacterized protein LOC117603868 isoform X1"
                     /calculated_mol_wt=26548
     CDS             1..235
                     /gene="LOC117603868"
                     /coded_by="XM_034323444.2:162..869"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds nvshfndtsn tfpeeltteq ttdvkqrvty
      181 ddlwaqhrds qmnpkykdfr gsrgmnters avppqresek tappemtyde kniws
//
LOCUS       XP_034179345             234 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179345
VERSION     XP_034179345.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323454.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179345.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..234
                     /product="uncharacterized protein LOC117603868 isoform X2"
                     /calculated_mol_wt=26461
     CDS             1..234
                     /gene="LOC117603868"
                     /coded_by="XM_034323454.2:241..945"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds nvshfndtsn tfpeeltteq ttdvkqrvty
      181 ddlwaqhrds qmnpkykdfr grgmntersa vppqresekt appemtydek niws
//
LOCUS       XP_034179354             223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179354
VERSION     XP_034179354.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179354.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="uncharacterized protein LOC117603868 isoform X3"
                     /calculated_mol_wt=25200
     CDS             1..223
                     /gene="LOC117603868"
                     /coded_by="XM_034323463.2:270..941"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds neeltteqtt dvkqrvtydd lwaqhrdsqm
      181 npkykdfrgs rgmntersav ppqresekta ppemtydekn iws
//
LOCUS       XP_034179364             222 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603868 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179364
VERSION     XP_034179364.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323473.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179364.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="uncharacterized protein LOC117603868 isoform X4"
                     /calculated_mol_wt=25113
     CDS             1..222
                     /gene="LOC117603868"
                     /coded_by="XM_034323473.2:262..930"
                     /db_xref="GeneID:117603868"
ORIGIN      
        1 mmyqenednv kiiqqlipga tlenlqafhk cmstdavyks glpcgitsgl mfhfllpkkl
       61 gaikpvvtal ssftaflwgk iiyspicker vfgpgdvfag klqekrdlhd nssyggstnq
      121 edqvnrltis sdklleddss wsnfdpflds neeltteqtt dvkqrvtydd lwaqhrdsqm
      181 npkykdfrgr gmntersavp pqresektap pemtydekni ws
//
LOCUS       XP_076546154             218 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600614 [Osmia lignaria lignaria].
ACCESSION   XP_076546154
VERSION     XP_076546154.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690039.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..218
                     /product="uncharacterized protein LOC117600614"
                     /calculated_mol_wt=25277
     CDS             1..218
                     /gene="LOC117600614"
                     /coded_by="XM_076690039.1:127..783"
                     /db_xref="GeneID:117600614"
ORIGIN      
        1 mfmfpepppl dyyitdisss ysadgifyhg raaaghkhia kqlkpikrtl rdpiappqel
       61 dsrtlsrilq kekggyvnpc gkddgmvydi lkwmpkwkrk grekfsfstf prsqqyelsh
      121 iretqskksg kckttetkre ekeeeeiktd kepcdflepp iwwkgetgkp ralpvgnidd
      181 ytferaadrd hadmkisekl hmdhpkkyip gvihrikv
//
LOCUS       XP_034179377             212 aa            linear   INV 03-SEP-2025
DEFINITION  hemK methyltransferase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034179377
VERSION     XP_034179377.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179377.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..212
                     /product="hemK methyltransferase 2"
                     /calculated_mol_wt=23816
     Region          14..210
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     Site            order(48..54,75..76,100..102,118)
                     /site_type="other"
                     /note="S-adenosylmethionine binding site [chemical
                     binding]"
                     /db_xref="CDD:100107"
     CDS             1..212
                     /gene="HemK2"
                     /coded_by="XM_034323486.2:1..639"
                     /db_xref="GeneID:117603898"
ORIGIN      
        1 mdtplvklst reletvyeps edsfllidal eadlevlkvk kpvmcleigs gsgivitala
       61 malskycqpy hlaidinlda ckatkktame nsveinaiqm dllscirgnr afdivlfnpp
      121 yvvtsdeeis nsklvfrtwa ggsngrkvme rvfpripdil sdvgifylli ikendpeyil
      181 ctfkhlnmeg rivaerkirg ehlyvlrfek it
//
LOCUS       XP_034172154             159 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600617 [Osmia lignaria lignaria].
ACCESSION   XP_034172154
VERSION     XP_034172154.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316263.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..159
                     /product="uncharacterized protein LOC117600617"
                     /calculated_mol_wt=17904
     Region          53..106
                     /region_name="PTN_MK_C"
                     /note="PTN/MK heparin-binding protein family, C-terminal
                     domain; pfam01091"
                     /db_xref="CDD:460059"
     Region          95..158
                     /region_name="PTN_MK_C"
                     /note="PTN/MK heparin-binding protein family, C-terminal
                     domain; pfam01091"
                     /db_xref="CDD:460059"
     CDS             1..159
                     /gene="LOC117600617"
                     /coded_by="XM_034316263.2:376..855"
                     /db_xref="GeneID:117600617"
ORIGIN      
        1 mklwwslalv giallmvvvt traesdlwee ddtevlvrtv rgtkerasgs tsscryvkgq
       61 wsecdsktnt rsrtlnlkkg dksceqtkti qkkckkacry ekgtwsgcan qlmtrvdnlk
      121 vnsdascekt rrltkrckpe tntkkstkge rsnkksgkq
//
LOCUS       XP_034179387             159 aa            linear   INV 03-SEP-2025
DEFINITION  SNAP associated protein [Osmia lignaria lignaria].
ACCESSION   XP_034179387
VERSION     XP_034179387.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179387.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..159
                     /product="SNAP associated protein"
                     /calculated_mol_wt=17922
     Region          26..113
                     /region_name="Snapin_Pallidin"
                     /note="Snapin/Pallidin; pfam14712"
                     /db_xref="CDD:464275"
     CDS             1..159
                     /gene="Snapin"
                     /coded_by="XM_034323496.2:49..528"
                     /db_xref="GeneID:117603906"
ORIGIN      
        1 mdvdtasdnt siddkiddfc enptrdalte glmsllkptv dqlderirat ricqielkqr
       61 ieslteelqr inealqcple tdsyvkklin akhkitivsn ilqntqerln kihqaveknt
      121 akrkalldhs ssygnvagvl dneeqteaek etaqskeqe
//
LOCUS       XP_034179400             158 aa            linear   INV 03-SEP-2025
DEFINITION  exocyst complex component secretory 8 [Osmia lignaria lignaria].
ACCESSION   XP_034179400
VERSION     XP_034179400.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323509.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..158
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..158
                     /product="exocyst complex component secretory 8"
                     /calculated_mol_wt=17688
     Region          <57..130
                     /region_name="Sec8_exocyst"
                     /note="Sec8 exocyst complex component specific domain;
                     pfam04048"
                     /db_xref="CDD:367782"
     CDS             1..158
                     /gene="Sec8"
                     /coded_by="XM_034323509.2:420..896"
                     /db_xref="GeneID:117603911"
ORIGIN      
        1 mnsvpptkpp rgikptkets gllisviral sasetneqre iekaklekey kksdqrldel
       61 vslhdqdlte vmqifsalse kvtasrekih avkenlnack qllrckreel lnlwlegieh
      121 khvlhllkdv tpdsctrmlp ltdtdslsva tsynaasd
//
LOCUS       XP_034172168             136 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600623 [Osmia lignaria lignaria].
ACCESSION   XP_034172168
VERSION     XP_034172168.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316277.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172168.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="uncharacterized protein LOC117600623"
                     /calculated_mol_wt=15843
     Region          7..>67
                     /region_name="DUF4604"
                     /note="Domain of unknown function (DUF4604); pfam15377"
                     /db_xref="CDD:464685"
     CDS             1..136
                     /gene="LOC117600623"
                     /coded_by="XM_034316277.2:295..705"
                     /db_xref="GeneID:117600623"
ORIGIN      
        1 msrkkhnvsy ikpnepkflr elkeqvgyke gptvetkrev lpvasdnede rdeekpvvvv
       61 lnsehlteee aeafkkkkee eetnapadls kriifrknkl tetesetidk pvkkkikkrk
      121 qektvlsfnd ndedyl
//
LOCUS       XP_034179413             108 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603919 [Osmia lignaria lignaria].
ACCESSION   XP_034179413
VERSION     XP_034179413.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323522.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..108
                     /product="uncharacterized protein LOC117603919"
                     /calculated_mol_wt=12036
     CDS             1..108
                     /gene="LOC117603919"
                     /coded_by="XM_034323522.2:1..327"
                     /db_xref="GeneID:117603919"
ORIGIN      
        1 mkgevllkml cdteinrdlk vprvlyfill liyisvqlsm tlsipttdfd tttlssnnst
       61 addlyvikav vyeigiltda dnttndtteg qeevklsfyn ppkknnep
//
LOCUS       XP_034177104              96 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602760 [Osmia lignaria lignaria].
ACCESSION   XP_034177104
VERSION     XP_034177104.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321213.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177104.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..96
                     /product="uncharacterized protein LOC117602760"
                     /calculated_mol_wt=10576
     Region          <41..84
                     /region_name="PRK05597"
                     /note="molybdopterin biosynthesis protein MoeB; Validated"
                     /db_xref="CDD:235526"
     CDS             1..96
                     /gene="LOC117602760"
                     /coded_by="XM_034321213.2:875..1165"
                     /db_xref="GeneID:117602760"
ORIGIN      
        1 meirtllifl sviasavtfp qkdgqifsne aikqaqntyl ipkdatiqkv qegielaaye
       61 sipgdqkinl feilgehvpp evvnnlqtqi dqigrn
//
LOCUS       XP_076546155              96 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602760 [Osmia lignaria lignaria].
ACCESSION   XP_076546155
VERSION     XP_076546155.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690040.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..96
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..96
                     /product="uncharacterized protein LOC117602760"
                     /calculated_mol_wt=10576
     Region          <41..84
                     /region_name="PRK05597"
                     /note="molybdopterin biosynthesis protein MoeB; Validated"
                     /db_xref="CDD:235526"
     CDS             1..96
                     /gene="LOC117602760"
                     /coded_by="XM_076690040.1:363..653"
                     /db_xref="GeneID:117602760"
ORIGIN      
        1 meirtllifl sviasavtfp qkdgqifsne aikqaqntyl ipkdatiqkv qegielaaye
       61 sipgdqkinl feilgehvpp evvnnlqtqi dqigrn
//
LOCUS       XP_034172240              67 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-directed RNA polymerases I, II, and III subunit Rpb10 [Osmia
            lignaria lignaria].
ACCESSION   XP_034172240
VERSION     XP_034172240.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316349.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..67
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..67
                     /product="DNA-directed RNA polymerases I, II, and III
                     subunit Rpb10"
                     /calculated_mol_wt=7500
     Region          1..67
                     /region_name="PLN00032"
                     /note="DNA-directed RNA polymerase; Provisional"
                     /db_xref="CDD:215034"
     CDS             1..67
                     /gene="Rpb10"
                     /coded_by="XM_034316349.2:43..246"
                     /db_xref="GeneID:117600648"
ORIGIN      
        1 miipvrcftc gkvignkwea ylgllqaeyt egdaldalgl kryccrrmll ghvdliekll
       61 nyaplek
//
LOCUS       XP_034177425            1303 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear pore complex protein Nup154 [Osmia lignaria lignaria].
ACCESSION   XP_034177425
VERSION     XP_034177425.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321534.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1303
                     /product="nuclear pore complex protein Nup154"
                     /calculated_mol_wt=146157
     Region          16..1241
                     /region_name="NUP170"
                     /note="Nuclear pore complex subunit [Intracellular
                     trafficking and secretion]; COG5308"
                     /db_xref="CDD:227624"
     CDS             1..1303
                     /gene="Nup154"
                     /coded_by="XM_034321534.2:107..4018"
                     /db_xref="GeneID:117602934"
ORIGIN      
        1 mtltemeplk ihldalemag kvvdthitad snfpslinlm rynvqggptv sglddhdyps
       61 sngmsvclsn inqmkfhski slpseimehf rhmqchcmmg lfteiskawl tidsdiyvws
      121 yenesdvayf dglnetiisv glvkpkpgvf qsyvkyllil tttveitilg vtltennegt
      181 ppemqlvpep iftvttdgig ittiantssg riflggrngs lyeiyyqaes swfgkrckki
      241 nhsegplsfl vpsfvtials eeeaiiqisv ddtryilytl gdkgtitvwd idndgaskva
      301 slsqaslvqn avhvvktlds nnfrplvsis aitesesvhl nlvvvaatgt rfyfsctsvv
      361 npttrpqglq lihvrlppgy aanatvmrpr kvqmahyrkg tlilvcggdt etalclsnda
      421 ypftnylaet qcplsldspv wamaeilvep airiekqsts qgepplivrq hmeaprkfif
      481 ltsqgaiiyv qvrpmdilke lllkqrgpdt eavrayfqlq sleqacatcl ilatlessqn
      541 aelaewatra fflygsqlst smgpaidlhn mntgdirtst prvpnydlrh qgfrphaplg
      601 fnteiplqqf sakhnglyly vgrilrpiwn mrcikqeiin nktqisstit trqvswilgl
      661 lqalrsflnk nthitkqhst trnipdgfet tiashyqepi veernslaal kifithacev
      721 lelwkilcen nlnnivncls kdqinqfsta tfrdliligh eisslliihl idsylgdnas
      781 vdivsqrlre vcpnlyrsed avcskaneii lkaksctnpe ekecylqsal klckevaprl
      841 nlsavcqqfv acqfysgvle lclccaermd pnnaalhyyk nnepiedqeg rfayikrfei
      901 ykefialldh lynqsisnpl tptipsrpgp ptqnsltapv tpaketllqm indalhspce
      961 ilhasvyewm ikrglhgelv afaapsleay ltrfnapdll wqfyeknknh aaaakildsl
     1021 atkesnipls qrveylarav vcmrsdqagy apylgiflre ledkvevari qqqildtinn
     1081 qglsdrlsee ahralkfsll ditklyekya dplqlweckl aiihcsghqd amliqeiwtn
     1141 iinnelkacs asspedkmai lmskiislgq eysasphcfp vdflikqlei kacnlnvlnt
     1201 giisgflqlg vsmedlldiy kmigkdtrtw lnegnefhli estanlvnyf ishsnitnnl
     1261 irrkiitkcq dviskclttl yskphgqeli tklrsiqsvl nrm
//
LOCUS       XP_034177438             972 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2-alpha kinase isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034177438
VERSION     XP_034177438.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321547.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177438.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..972
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase isoform X1"
                     /calculated_mol_wt=110620
     Region          43..313
                     /region_name="Luminal_IRE1_like"
                     /note="The Luminal domain, a dimerization domain, of
                     Inositol-requiring protein 1-like proteins; cl14874"
                     /db_xref="CDD:276097"
     Site            order(124..128,133,135..138,140..142,154,156..158,
                     179..180,188,190,205,207..209)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:188873"
     Region          509..924
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(521..524,527,529,542,544,574,744..747,790,794..795,
                     797,807..808)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..972
                     /gene="LOC117602938"
                     /coded_by="XM_034321547.2:6..2924"
                     /db_xref="GeneID:117602938"
ORIGIN      
        1 msvtdlwkri wlcslcmvfl ifplidtsdi kqltfceqks srsllfistl dgkisaldan
       61 nsgkkqwtld lnegpmlsss ihrrelnnng qwirlipsln gglykfdgen leaipvtanq
      121 llhssfrysd dlvfsggrev ksygissttg kilyecgihg ctnstqkeaf veqemlivqr
      181 fqqtvravep rtgierwnfs vgqhdltlvp qsdvycqnkv qshnldieik vvipdgliwa
      241 inknnptvkl wqqkfdspiv siwredantk sgeynnlkei nlfdskqwsw gtefstspgi
      301 ylgmhdrqly iqengelhks leisqkhvqh skypwqpypa vghavkktlp sptndnndda
      361 llqisdaqst talsvlynse yvngngfyly skdqlqlgnd kqcghqnpil llidkeekvy
      421 isndthgedd dtpvqiiivs lwywwkevlv isittailln fmltqrllna ttlakdavlp
      481 pliverhiet nksniqqsdd gknsddfksr yltdfepvdc lgkggygvvf eaknkiddcn
      541 yaikrialpn skysrervmr evkalakldh qnivryfnaw lecppagwqe khdpqcmnkl
      601 mspnseftad itpsitkinn svcinvtqtd qssvdsacea yeinridsne dsfiifekph
      661 svgsnediin irncssgssn tsisnnivgk elpttddses ivfedteskr sekenkrkrq
      721 asfslnlnks nsykspkmfl yiqmqlcqrl slrewlkiqs trdyrrvlni fqqivdavey
      781 vhlqglihrd lkpsniffsf ddkikvgdfg lvtamtedyd eahtpaened itlknslhta
      841 yvgthlymsp eqingqgyny kvdiyslgii lfellipfvt emerittlin lrksifpnnf
      901 gndypsehnl lnmmldenpn krpttfgika kapllndeia ngfgtsedtk whfelpqltr
      961 hssvissssn ds
//
LOCUS       XP_034177456             941 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2-alpha kinase isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034177456
VERSION     XP_034177456.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321565.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177456.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..941
                     /product="eukaryotic translation initiation factor 2-alpha
                     kinase isoform X2"
                     /calculated_mol_wt=107013
     Region          43..282
                     /region_name="Luminal_IRE1_like"
                     /note="The Luminal domain, a dimerization domain, of
                     Inositol-requiring protein 1-like proteins; cl14874"
                     /db_xref="CDD:276097"
     Site            order(124..128,133,135..138,140..142,154,156..158,
                     179..180,188,190,205,207..209)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:188873"
     Region          478..893
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(490..493,496,498,511,513,543,713..716,759,763..764,
                     766,776..777)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..941
                     /gene="LOC117602938"
                     /coded_by="XM_034321565.2:6..2831"
                     /db_xref="GeneID:117602938"
ORIGIN      
        1 msvtdlwkri wlcslcmvfl ifplidtsdi kqltfceqks srsllfistl dgkisaldan
       61 nsgkkqwtld lnegpmlsss ihrrelnnng qwirlipsln gglykfdgen leaipvtanq
      121 llhssfrysd dlvfsggrev ksygissttg kilyecgihg ctnstqkeaf veqemlivqr
      181 fqqtvravep rtgierwnfs vgqhdltlvp qsdvycqnkv qshnldieik vvipdgliwa
      241 inknnptvkl wqqkfdspiv siwredantk sgmhdrqlyi qengelhksl eisqkhvqhs
      301 kypwqpypav ghavkktlps ptndnnddal lqisdaqstt alsvlynsey vngngfylys
      361 kdqlqlgndk qcghqnpill lidkeekvyi sndthgeddd tpvqiiivsl wywwkevlvi
      421 sittaillnf mltqrllnat tlakdavlpp liverhietn ksniqqsddg knsddfksry
      481 ltdfepvdcl gkggygvvfe aknkiddcny aikrialpns kysrervmre vkalakldhq
      541 nivryfnawl ecppagwqek hdpqcmnklm spnseftadi tpsitkinns vcinvtqtdq
      601 ssvdsaceay einridsned sfiifekphs vgsnediini rncssgssnt sisnnivgke
      661 lpttddsesi vfedteskrs ekenkrkrqa sfslnlnksn sykspkmfly iqmqlcqrls
      721 lrewlkiqst rdyrrvlnif qqivdaveyv hlqglihrdl kpsniffsfd dkikvgdfgl
      781 vtamtedyde ahtpaenedi tlknslhtay vgthlymspe qingqgynyk vdiyslgiil
      841 fellipfvte merittlinl rksifpnnfg ndypsehnll nmmldenpnk rpttfgikak
      901 apllndeian gfgtsedtkw hfelpqltrh ssvissssnd s
//
LOCUS       XP_034177469             916 aa            linear   INV 03-SEP-2025
DEFINITION  RING finger and CCCH-type zinc finger domain-containing protein
            roquin [Osmia lignaria lignaria].
ACCESSION   XP_034177469
VERSION     XP_034177469.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321578.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..916
                     /product="RING finger and CCCH-type zinc finger
                     domain-containing protein roquin"
                     /calculated_mol_wt=102425
     Region          6..54
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(14,17,33,35,38,41,50,53)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          270..325
                     /region_name="ROQ_II"
                     /note="Roquin II domain; pfam18386"
                     /db_xref="CDD:436456"
     Region          416..442
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     CDS             1..916
                     /gene="roq"
                     /coded_by="XM_034321578.2:173..2923"
                     /db_xref="GeneID:117602953"
ORIGIN      
        1 mpiqapqwte flscpvcchd fdvairgpis lgcghticrt clanlhrkqc pfdqsvinte
       61 ierlpvneal lqlvgnsvpt nvatayqkll ppedhanylv akrcieelal ylkpcqtnpi
      121 latgaglvsr pmqrklvtlv gcqlvepegr aravraarsl gersvtelil qhqnpqqlna
      181 nlwaavrarg cqflgpamqe evlklvllal edgsalsrkv lvmfvvqrle shfpqaskts
      241 ighvvqllyr ascfkvskre gdsslmqlke efrtyealrr ehdaqivqia teaglriapd
      301 qwsallygdt shkshmqsii dklqtpqsfa qsvqelvial qrtpdpgqls slrpqlella
      361 sidpspetep ptlaecraal eavrtvvaal vefiqqhgnr ktqdgthnvg pgqpkykvsm
      421 crdlalrgsc prstnctfah sdmeldkyrs knrklsirsn lpvtnnsdtk tdkvvtgsnn
      481 ksfkqndels yhsikshdnt hrdnfnsvnk vnpmqhhttt nennfvgslt nymlpspqgi
      541 vpmvptknid mhcshyivpn apieyttpnl gmwdssqiss nvtngisnsk kqrtvaktla
      601 mllqrrmeil nqletivnkq vpqmksnydv qgdalssnfs iwantqsnpi ippsnmncnn
      661 nfpctdsilf ddefvpfdas ssnkygpisk lpknlirssn gvqptnfyge ggtppamsay
      721 rpmpntnsvt swyygnhqpy atiganggiq sinntgfedn yitfgrnise stthtqfars
      781 ecmskdlile sqkvkqqlkh lekkindlkl atqgatftag erlaqelqmi eagirekekd
      841 vrnwspygti pwiqssnnvl gsqnfnqdyk peeedtyeag iandmrelel rwefelqeqe
      901 lqelqhwsse ednskk
//
LOCUS       XP_076546157             709 aa            linear   INV 03-SEP-2025
DEFINITION  armadillo repeat-containing protein 8 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546157
VERSION     XP_076546157.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690042.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..709
                     /product="armadillo repeat-containing protein 8 isoform
                     X1"
                     /calculated_mol_wt=78197
     Region          47..87
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <50..>112
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          409..434
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          <427..>573
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Region          <471..>695
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Site            order(478,482,486,520,524,528,563,567,571)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          495..531
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          538..572
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          583..618
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          626..660
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..709
                     /gene="LOC117602959"
                     /coded_by="XM_076690042.1:290..2419"
                     /db_xref="GeneID:117602959"
ORIGIN      
        1 mvsvmqpfmd vessrsyide lysldpqkcl eaiiclknsv igsnrqkgsv iaqgvvprll
       61 qllgdsadri gdnirleaav tlgslakgtd qhvlalidlg vvpllfqvil stpvsetple
      121 gkpkvtdlln eaclrclrtv fqhpaapvhs iyqdpalvpr lvslanqsvt cqicvatilt
      181 tackttedqn alskggavet lamqldsplp dvqlsalacl anmcyknyrv cvmvasatts
      241 ntpqgrlvpv algqlmgrek ssliqleaar cvtymhrtgv llykdpriiy ralpclvrlc
      301 hrdhpprerv aaaetlaflt evntelqrla sisnhliptl aellrphphv simvlinvsi
      361 cmyivinifq vqdatltqdm rqaafrafas lgandedirk riieteslme qvvaglqdpg
      421 gshvrlaavr clhslsrsvq qlrttfqdha vwrplmqllh gadkglegrg esevdlltva
      481 sstlcnllle fspskepile sggiellcsl tkrctpalrl ngiwalmnva fqaeqqvklq
      541 ilqclgtdqi fclladqdip vlmktlgllr nllstkahid rimgehaahv mqavilvled
      601 prhpadvkeq alcilanvad girakdhima nedvlkklmd ymmhsnvklq vaavfcvcnl
      661 vrreepgaaq rqarlkelgf yrilqqlrqs kdhqlyekvr tavnefyda
//
LOCUS       XP_034177507             688 aa            linear   INV 03-SEP-2025
DEFINITION  armadillo repeat-containing protein 8 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177507
VERSION     XP_034177507.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321616.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..688
                     /product="armadillo repeat-containing protein 8 isoform
                     X2"
                     /calculated_mol_wt=75802
     Region          47..87
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          <50..>112
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          <406..>552
                     /region_name="COG5369"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:227663"
     Region          <450..>674
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Site            order(457,461,465,499,503,507,542,546,550)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          474..510
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          517..551
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          558..599
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          605..639
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..688
                     /gene="LOC117602959"
                     /coded_by="XM_034321616.2:290..2356"
                     /db_xref="GeneID:117602959"
ORIGIN      
        1 mvsvmqpfmd vessrsyide lysldpqkcl eaiiclknsv igsnrqkgsv iaqgvvprll
       61 qllgdsadri gdnirleaav tlgslakgtd qhvlalidlg vvpllfqvil stpvsetple
      121 gkpkvtdlln eaclrclrtv fqhpaapvhs iyqdpalvpr lvslanqsvt cqicvatilt
      181 tackttedqn alskggavet lamqldsplp dvqlsalacl anmcyknyrv cvmvasatts
      241 ntpqgrlvpv algqlmgrek ssliqleaar cvtymhrtgv llykdpriiy ralpclvrlc
      301 hrdhpprerv aaaetlaflt evntelqrla sisnhliptl aellrphphv qdatltqdmr
      361 qaafrafasl gandedirkr iieteslmeq vvaglqdpgg shvrlaavrc lhslsrsvqq
      421 lrttfqdhav wrplmqllhg adkglegrge sevdlltvas stlcnlllef spskepiles
      481 ggiellcslt krctpalrln giwalmnvaf qaeqqvklqi lqclgtdqif clladqdipv
      541 lmktlgllrn llstkahidr imgehaahvm qavilvledp rhpadvkeqa lcilanvadg
      601 irakdhiman edvlkklmdy mmhsnvklqv aavfcvcnlv rreepgaaqr qarlkelgfy
      661 rilqqlrqsk dhqlyekvrt avnefyda
//
LOCUS       XP_034177525             602 aa            linear   INV 03-SEP-2025
DEFINITION  actin-interacting protein 1 flr [Osmia lignaria lignaria].
ACCESSION   XP_034177525
VERSION     XP_034177525.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321634.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177525.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="actin-interacting protein 1 flr"
                     /calculated_mol_wt=66076
     Region          10..391
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          62..100
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          105..143
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          150..186
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          192..228
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          236..274
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          281..300
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          311..598
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(318,336,340,346..347,360..361,377,381,387..388,419,
                     438,456,461,467,470,482,500,504,510..511,525..526,543,548,
                     554..555,568..569,587,591,597..598)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          324..358
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          365..385
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          434..481
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          486..516
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          530..567
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          574..597
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..602
                     /gene="flr"
                     /coded_by="XM_034321634.2:153..1961"
                     /db_xref="GeneID:117602981"
ORIGIN      
        1 msyetkyifa tlprtqrgqp lvlggdpngk nflytngnsv iirnidnpai adiytehscp
       61 vnvakyspsg fyiasgdqsg kvriwdtvnk ehilknefhp iggpikdiaw spdnqrmvvv
      121 gegrerfghv fmaetgtsvg eisgqskpin scdfrpakpf rlitgsednt iaifegppfk
      181 fkmtkqehar fvqavrysps gnlfasagfd gkvfiydgts sdlvgevgsp ahqggvygva
      241 wkpdgtqllt asgdktcklw dvetrslise fnmgstvddq qvsclwqgnh llsvslsgfi
      301 nyldvdnpak piriikghnk pitvltlssd rntiytgshd gyitswnant gendrvlgqg
      361 hgnqingmkt aknilytvgi ddtlrsvdvd tntytdtcvv kldsqprgid iyndivvivt
      421 vrqitvtqdg rkvssvpidy epscvsinqe ngdvavgats nntihiytls gtdlspktel
      481 ehlgsvtdva yspdskylva cdtnrkvvly tvseyklahn rewgfhnarv nsvawspdsi
      541 mvasgsldtt iiiwsvtnpa khtiiknahp qsqitrlvwl deetlisvgq dcntkiwrie
      601 ki
//
LOCUS       XP_034177541             571 aa            linear   INV 03-SEP-2025
DEFINITION  USP6 N-terminal-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034177541
VERSION     XP_034177541.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321650.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..571
                     /product="USP6 N-terminal-like protein"
                     /calculated_mol_wt=66027
     Region          98..313
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..571
                     /gene="RN-tre"
                     /coded_by="XM_034321650.2:584..2299"
                     /db_xref="GeneID:117602989"
ORIGIN      
        1 mneeellkrs aaerdrifsc ydrgrengae idpwedpsfe vyhatdrygf ihdkrlpqkl
       61 dpneikthrv emerlkkwek mtkqwdssst keklrrrvyk gipdrfrgqv walllgiknl
      121 kkeqagkyee mlqlarqwst eirqidadva rqyrdhinyr erysikqrsm fyvlaaysmy
      181 nmevgycqgm svlaglllly mdeedafwgl svlladkkyt mhgfyvdgfp klnrfiehhd
      241 kimnkflpkl krkldkcgcd silyalkwff vvfqertpvs lglriwdifl ldgdrilpam
      301 aytvmkmhkr flmpmeslde fcnylqikle kdfcfdddtv inamersmde lkrakldypg
      361 pplphelprf pfgtfkeptf askvgrraee fseaqhvmre sitqrrdtvv pdddresttp
      421 veqtscglgg skfsfdpsld dgassngsrr sladtsvtst aclsvfssat rsqaldnsld
      481 tqsnisndss gsgglptpra tphqpspdvv riyvpytspi sgsykddlpr tlprsletnr
      541 irirvdpdqt pivenlkpfs lenpeveldl k
//
LOCUS       XP_034177551             571 aa            linear   INV 03-SEP-2025
DEFINITION  USP6 N-terminal-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034177551
VERSION     XP_034177551.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..571
                     /product="USP6 N-terminal-like protein"
                     /calculated_mol_wt=66027
     Region          98..313
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..571
                     /gene="RN-tre"
                     /coded_by="XM_034321660.2:454..2169"
                     /db_xref="GeneID:117602989"
ORIGIN      
        1 mneeellkrs aaerdrifsc ydrgrengae idpwedpsfe vyhatdrygf ihdkrlpqkl
       61 dpneikthrv emerlkkwek mtkqwdssst keklrrrvyk gipdrfrgqv walllgiknl
      121 kkeqagkyee mlqlarqwst eirqidadva rqyrdhinyr erysikqrsm fyvlaaysmy
      181 nmevgycqgm svlaglllly mdeedafwgl svlladkkyt mhgfyvdgfp klnrfiehhd
      241 kimnkflpkl krkldkcgcd silyalkwff vvfqertpvs lglriwdifl ldgdrilpam
      301 aytvmkmhkr flmpmeslde fcnylqikle kdfcfdddtv inamersmde lkrakldypg
      361 pplphelprf pfgtfkeptf askvgrraee fseaqhvmre sitqrrdtvv pdddresttp
      421 veqtscglgg skfsfdpsld dgassngsrr sladtsvtst aclsvfssat rsqaldnsld
      481 tqsnisndss gsgglptpra tphqpspdvv riyvpytspi sgsykddlpr tlprsletnr
      541 irirvdpdqt pivenlkpfs lenpeveldl k
//
LOCUS       XP_034177572             552 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602999 [Osmia lignaria lignaria].
ACCESSION   XP_034177572
VERSION     XP_034177572.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321681.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177572.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..552
                     /product="uncharacterized protein LOC117602999"
                     /calculated_mol_wt=63437
     Region          52..>341
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          69..92
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          93..116
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          117..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          141..164
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          165..188
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          189..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          213..254
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          255..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          278..298
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          324..337
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          345..373
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          374..397
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..552
                     /gene="LOC117602999"
                     /coded_by="XM_034321681.2:34..1692"
                     /db_xref="GeneID:117602999"
ORIGIN      
        1 mknimfihly ligfyilcit qrfaceicst clcltaineg piidchgkdl enndmliadl
       61 ltlngyrsln klilsnnnis nlpehllkkl kpvksldlse nlmetihtsi fidlsnledl
      121 nlsknkltvf ddsllkvlpt lltlnvshnh ihtvesvadk ttvniiyltl shnnisslpk
      181 kffellpnlq yldlsfnrih slmdysltrl nslkmisinn nfltsldiri fpksliklhs
      241 ghnqiaeify essqlkvlni eynniseiyk nitklneltd lnvagntlyd fpntllknlr
      301 kldlsnnklq yipeslsvqn fplllelnvs knpiqnltfr sdlqlhsfia snistlktid
      361 kdtfthfrmp ptdcirvtis nnemlsfihe dalkhlklcf ldlrnnrlsy vsqelvmnsd
      421 tsvvldinlq gnpfkcncsl qwmlndlips lyftrpdlld nlrcawppqi snirmvhwyg
      481 wkeqifcnms nlnenlimkv dnvvdkkvtf esstgwlivm gialtvlssl iiigiiwtqk
      541 iaikkrrinr kf
//
LOCUS       XP_034177606             477 aa            linear   INV 03-SEP-2025
DEFINITION  short Calcium-binding Mitochondrial Carrier isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177606
VERSION     XP_034177606.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321715.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="short Calcium-binding Mitochondrial Carrier
                     isoform X1"
                     /calculated_mol_wt=54007
     Region          26..160
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     Region          202..453
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..477
                     /gene="SCaMC"
                     /coded_by="XM_034321715.2:173..1606"
                     /db_xref="GeneID:117603017"
ORIGIN      
        1 mpdnrgnvvh gvmpphylhk lpaedeerle nifqkldldg ngridvkdls kalrevgvdk
       61 syaekflars dstksgdisl aefihyvreh eknlrlqfsh ldrnkdgkid leelvksfee
      121 lgitmdysea krllqrmdtd gsltitfnew rdfllyapss dlpglieywh htnymdiged
      181 iavpedfttn emvsgmwwrh lvaggvagiv srtctapldr ikvylqvhgt rhckirscfs
      241 ymlreggyss lwrgnginvl kiapesalkf layeqikryi kgdnvrelei herllagsla
      301 ggisqstiyp levlktrfal rktgefsglv daikkiyrqg glksfykgyi pnllgilpya
      361 gidlavyetl knwylrnhdk serpsvyill fcgtassttg qvcsyplalv rtrlqanais
      421 dkypntmigv fkeilrnegl pglyrgitpn flkvapavsi syvvyeicre llgvnmt
//
LOCUS       XP_034177611             353 aa            linear   INV 03-SEP-2025
DEFINITION  short Calcium-binding Mitochondrial Carrier isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177611
VERSION     XP_034177611.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321720.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="short Calcium-binding Mitochondrial Carrier
                     isoform X2"
                     /calculated_mol_wt=39768
     Region          78..329
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..353
                     /gene="SCaMC"
                     /coded_by="XM_034321720.2:439..1500"
                     /db_xref="GeneID:117603017"
ORIGIN      
        1 mdyseakrll qrmdtdgslt itfnewrdfl lyapssdlpg lieywhhtny mdigediavp
       61 edfttnemvs gmwwrhlvag gvagivsrtc tapldrikvy lqvhgtrhck irscfsymlr
      121 eggysslwrg nginvlkiap esalkflaye qikryikgdn vreleiherl lagslaggis
      181 qstiyplevl ktrfalrktg efsglvdaik kiyrqgglks fykgyipnll gilpyagidl
      241 avyetlknwy lrnhdkserp svyillfcgt assttgqvcs yplalvrtrl qanaisdkyp
      301 ntmigvfkei lrneglpgly rgitpnflkv apavsisyvv yeicrellgv nmt
//
LOCUS       XP_034177624             453 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603031 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177624
VERSION     XP_034177624.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321733.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177624.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="uncharacterized protein LOC117603031 isoform X1"
                     /calculated_mol_wt=52183
     Region          22..425
                     /region_name="CDC27"
                     /note="DNA polymerase subunit Cdc27; pfam09507"
                     /db_xref="CDD:462819"
     CDS             1..453
                     /gene="LOC117603031"
                     /coded_by="XM_034321733.2:71..1432"
                     /db_xref="GeneID:117603031"
ORIGIN      
        1 miiesldkyl etvagyifdd dkvvtykwls kelevhvdma kqilwkfwqr ykeeksfdct
       61 fllmgilhdd gmrvevvkek dlataeekya kilsehiysl qkalakvqll emtddgdvry
      121 saikciesne rsneemhilr wgaksnevqp vseekinyvs epvkmkeeis pekksmdkkk
      181 naqkkgfdsl fgkvankqkt istlsntkkv etdstkqtls kedvpkpqkk naqgglssfl
      241 qqnkieekvk ntaspesntn tdcvlkdite ketilenkke rnknrhgkkr nrskdissat
      301 krrkritiqs dssdtddkee nvelisdeer kkesspetip pvkvssdtpp qakvengkrk
      361 vlktvdqtfe edgflvtkkv hvyescsede sevveskktv vpephpeikg kknnkqttlm
      421 nffkkdeidt hslakfiryv itetermlhp kye
//
LOCUS       XP_034177634             426 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603031 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177634
VERSION     XP_034177634.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321743.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177634.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="uncharacterized protein LOC117603031 isoform X2"
                     /calculated_mol_wt=48865
     Region          22..425
                     /region_name="CDC27"
                     /note="DNA polymerase subunit Cdc27; pfam09507"
                     /db_xref="CDD:462819"
     CDS             1..426
                     /gene="LOC117603031"
                     /coded_by="XM_034321743.2:71..1351"
                     /db_xref="GeneID:117603031"
ORIGIN      
        1 miiesldkyl etvagyifdd dkvvtykwls kelevhvdma kqilwkfwqr ykeeksfdct
       61 fllmgilhdd gmrvevvkek dlataeekya kilsehiysl qkalakvqll emtddgdvry
      121 saikciesne rsneemhilr wgaksnevqp vseekinyvs epvkmkeeis pekksmdkkk
      181 naqkkgfdsl fgkvankqkt istlsntkkv etdstkqtls kedvpkpqkk naqgglssfl
      241 qqnkieekvk ntaspesntn tdcvlkdite ketilenkke rnknrhgkkr nrskdissat
      301 krrkritiqs dssdtddkee nvelisdeer kkesspetip pvkvssdtpp qakvengkrk
      361 vlktvdqtfe edgflvtkkv hvyescsede sevveskktv vpephpeikg kknnkqttlm
      421 nffkkv
//
LOCUS       XP_034177595             543 aa            linear   INV 03-SEP-2025
DEFINITION  islet cell autoantigen 1-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034177595
VERSION     XP_034177595.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321704.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177595.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..543
                     /product="islet cell autoantigen 1-like protein"
                     /calculated_mol_wt=61040
     Region          61..264
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(70,73..74,78,80..81,84..85,87..88,98..99,101..102,
                     105..106,108..109,112,124,127..128,225,228..229,231,236,
                     239..240,242..243,246..247,250..251,253..254,257..258,
                     260..261)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     CDS             1..543
                     /gene="ICA69"
                     /coded_by="XM_034321704.2:16..1647"
                     /db_xref="GeneID:117603007"
ORIGIN      
        1 mntynkngsg vsgnafdcwv qrtnmhddsa itkmqhqfwv tkqalsrklg kkeddcivas
       61 daeldaklel frsiqescsy lqrvidkyqe rlctlaqeen amgrflkdag kqdktragkm
      121 msavgkslsy sgqqrlalra plgrlyqeve tfrqraiedt lqkvqameks rteyraalsw
      181 mknisqeldp dtskqlerfr kvqtrvrqgk ivfdnlaldc lqkvdllaaa rcnmfshalv
      241 lyqstllnft kksaqaysti assfkgyqry dfmvvrelae tstklaqetg gdddlddkdk
      301 lsffdmdfhd nveeaeevkp ttqgtvesnk qdeklldiec etknileleg ldmdsssskn
      361 gvtestsnik hkddvdelfk sltldnnmcr nnkqstkste egnqitfdes ltmdifdksd
      421 tnfdltdfsl evqnqgeqsl qsltsenivl lndlfgdkqn teseweaams ndtflppnil
      481 kqslgdaalg igqksilttn tgdkkssksg kqkgnswldl faeldplann pmenlsndsn
      541 asa
//
LOCUS       XP_034177643             342 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603040 [Osmia lignaria lignaria].
ACCESSION   XP_034177643
VERSION     XP_034177643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321752.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..342
                     /product="uncharacterized protein LOC117603040"
                     /calculated_mol_wt=38207
     Region          30..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          277..298
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          278..298
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(285..287,289..290,294,310,313..317,319..320)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          305..326
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..342
                     /gene="LOC117603040"
                     /coded_by="XM_034321752.2:279..1307"
                     /db_xref="GeneID:117603040"
ORIGIN      
        1 megqqfclrw hnfqntllss lpklldggyl tdvtlsaggr hihahkiils acsyyfkelf
       61 kdlsslqhpv ivlpgmeyan lcalvtfmyn gevniyqeql pallamadtl hirgladiag
      121 kntrhdngmy vqppppqlqe kiltetpikk enkdnvmsgg esletvleae aaenieesft
      181 dpesipycvk tkpyysinak lnqrekvsip svntssnkyp ekgyseggmd estpiledqi
      241 tpaedtkppp vwdanfkflt ssisgrtsqn ynkssvtcli cgkqlsnqyn lrvhmethsn
      301 ssysctacsh vsrsrdalrk hvsyrhpvas pqkrsryntp kp
//
LOCUS       XP_034177656             340 aa            linear   INV 03-SEP-2025
DEFINITION  abhydrolase domain containing 13-like protein Bem46 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034177656
VERSION     XP_034177656.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321765.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="abhydrolase domain containing 13-like protein
                     Bem46 isoform X1"
                     /calculated_mol_wt=38434
     Region          77..306
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..340
                     /gene="Bem46"
                     /coded_by="XM_034321765.2:267..1289"
                     /db_xref="GeneID:117603045"
ORIGIN      
        1 msfcvrlars kpirllggia mkcwafsgty ilacfllywl yggisaffll cfattgilyh
       61 redqlvyrpe wpansrvyvp apsifnlpyq siytrskdgt mlhmffisqp edkakkvpti
      121 lflhgnagnm ghrlqnavgl yhniqcnilm leyrgyglsq gspseeglym dacagidyls
      181 srtdintsei ivfgrslgga vainlatkpe nfqriwcliv entftsipdm aaylfgsrfl
      241 qylplflykn kylsihkvrs vtvptlfisg ladtlvpprm mqelhkncrs scknilsisg
      301 gthnetwcqp gyyqnicnfl selrenpvqv tsshwqiddi
//
LOCUS       XP_034177663             340 aa            linear   INV 03-SEP-2025
DEFINITION  abhydrolase domain containing 13-like protein Bem46 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034177663
VERSION     XP_034177663.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321772.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="abhydrolase domain containing 13-like protein
                     Bem46 isoform X1"
                     /calculated_mol_wt=38434
     Region          77..306
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..340
                     /gene="Bem46"
                     /coded_by="XM_034321772.2:200..1222"
                     /db_xref="GeneID:117603045"
ORIGIN      
        1 msfcvrlars kpirllggia mkcwafsgty ilacfllywl yggisaffll cfattgilyh
       61 redqlvyrpe wpansrvyvp apsifnlpyq siytrskdgt mlhmffisqp edkakkvpti
      121 lflhgnagnm ghrlqnavgl yhniqcnilm leyrgyglsq gspseeglym dacagidyls
      181 srtdintsei ivfgrslgga vainlatkpe nfqriwcliv entftsipdm aaylfgsrfl
      241 qylplflykn kylsihkvrs vtvptlfisg ladtlvpprm mqelhkncrs scknilsisg
      301 gthnetwcqp gyyqnicnfl selrenpvqv tsshwqiddi
//
LOCUS       XP_034177672             295 aa            linear   INV 03-SEP-2025
DEFINITION  abhydrolase domain containing 13-like protein Bem46 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034177672
VERSION     XP_034177672.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321781.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="abhydrolase domain containing 13-like protein
                     Bem46 isoform X2"
                     /calculated_mol_wt=33419
     Region          32..261
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..295
                     /gene="Bem46"
                     /coded_by="XM_034321781.2:106..993"
                     /db_xref="GeneID:117603045"
ORIGIN      
        1 mevvqsvemq gilyhredql vyrpewpans rvyvpapsif nlpyqsiytr skdgtmlhmf
       61 fisqpedkak kvptilflhg nagnmghrlq navglyhniq cnilmleyrg yglsqgspse
      121 eglymdacag idylssrtdi ntseiivfgr slggavainl atkpenfqri wcliventft
      181 sipdmaaylf gsrflqylpl flyknkylsi hkvrsvtvpt lfisgladtl vpprmmqelh
      241 kncrssckni lsisggthne twcqpgyyqn icnflselre npvqvtsshw qiddi
//
LOCUS       XP_034177685             313 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial carrier homolog 2 [Osmia lignaria lignaria].
ACCESSION   XP_034177685
VERSION     XP_034177685.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321794.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..313
                     /product="mitochondrial carrier homolog 2"
                     /calculated_mol_wt=35247
     Region          132..>196
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          <136..278
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..313
                     /gene="LOC117603058"
                     /coded_by="XM_034321794.2:456..1397"
                     /db_xref="GeneID:117603058"
ORIGIN      
        1 mippedeplw pdialrmltn tvshpieyak vliqigyepi pprptttllg qpalalpnvf
       61 qyvryiksvd gftgcycgli pklcaytvsa vafektskim kfsderntes ydecqreeie
      121 rknciyqfir dlisrtigii lshpldvial rmmaqfvgge rkynglfrsf vevykengvm
      181 gyyaglvprl ignaaalvlv ssstylidky visdralkpf vvstmrfvat titypflvvs
      241 hcmavnncgl iaglppqmpv ynswldcwsh lsatnqlkrg nsliwryytg pqviingkpi
      301 pinkynfqlq stt
//
LOCUS       XP_034193168             247 aa            linear   INV 03-SEP-2025
DEFINITION  rhythmically expressed gene 5 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193168
VERSION     XP_034193168.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337277.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193168.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..247
                     /product="rhythmically expressed gene 5 isoform X1"
                     /calculated_mol_wt=28179
     CDS             1..247
                     /gene="Reg-5"
                     /coded_by="XM_034337277.2:26..769"
                     /db_xref="GeneID:117610176"
ORIGIN      
        1 msnlrtyvlf aaililgcht lcatasaipm weflsrdekm sylfrvfsqq vatfcvdssm
       61 pdcnknllvs glrnlanmdd naldkldpyq rdakeiiwga mvkneystrv ahepnesyfa
      121 tgtdsvatsg setnglgeet isehtgpylv gpmvirvypd grpvpedsmr plpqdedike
      181 frhsrvpsve eieanrnvfy ekqskgnsyp vrrnnrqaye espcfrkdds lfkrrllqei
      241 vkrklry
//
LOCUS       XP_034193257             217 aa            linear   INV 03-SEP-2025
DEFINITION  rhythmically expressed gene 5 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193257
VERSION     XP_034193257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="rhythmically expressed gene 5 isoform X2"
                     /calculated_mol_wt=24866
     CDS             1..217
                     /gene="Reg-5"
                     /coded_by="XM_034337366.2:26..679"
                     /db_xref="GeneID:117610176"
ORIGIN      
        1 msnlrtyvlf aaililgcht lcatasaipm weflsrdekm sylfrvfsqq vatfcvdssm
       61 pdcnknllvs glrnlanmdd naldkldpyq rdakeittsg setnglgeet isehtgpylv
      121 gpmvirvypd grpvpedsmr plpqdedike frhsrvpsve eieanrnvfy ekqskgnsyp
      181 vrrnnrqaye espcfrkdds lfkrrllqei vkrklry
//
LOCUS       XP_034193343             208 aa            linear   INV 03-SEP-2025
DEFINITION  rhythmically expressed gene 5 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193343
VERSION     XP_034193343.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337452.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193343.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="rhythmically expressed gene 5 isoform X3"
                     /calculated_mol_wt=23810
     CDS             1..208
                     /gene="Reg-5"
                     /coded_by="XM_034337452.2:122..748"
                     /db_xref="GeneID:117610176"
ORIGIN      
        1 msylfrvfsq qvatfcvdss mpdcnknllv sglrnlanmd dnaldkldpy qrdakeiiwg
       61 amvkneystr vahepnesyf atgtdsvats gsetnglgee tisehtgpyl vgpmvirvyp
      121 dgrpvpedsm rplpqdedik efrhsrvpsv eeieanrnvf yekqskgnsy pvrrnnrqay
      181 eespcfrkdd slfkrrllqe ivkrklry
//
LOCUS       XP_034177693             237 aa            linear   INV 03-SEP-2025
DEFINITION  6-phosphogluconolactonase [Osmia lignaria lignaria].
ACCESSION   XP_034177693
VERSION     XP_034177693.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321802.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="6-phosphogluconolactonase"
                     /calculated_mol_wt=26215
     Region          14..227
                     /region_name="6PGL"
                     /note="6-Phosphogluconolactonase (6PGL) subfamily; 6PGL
                     catalyzes the second step of the oxidative phase of the
                     pentose phosphate pathway, the hydrolyzation of
                     6-phosphoglucono-1,5-lactone (delta form) to
                     6-phosphogluconate. 6PGL is thought to guard against
                     the...; cd01400"
                     /db_xref="CDD:238694"
     Site            order(43..44,70,143,174,197)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238694"
     CDS             1..237
                     /gene="Pgls"
                     /coded_by="XM_034321802.2:22..735"
                     /db_xref="GeneID:117603064"
ORIGIN      
        1 mekiliepqn ciivsylsnl lqtsaeeafa ngdtlkvgls ggsvvnllaq slpnittdws
       61 kwyfffcder ivpfdsndst ygeyksklig kipvtedqfi kinpdlsaed aakdyikkms
      121 vffppdrlpc fdvlllgvgp dghtcslfpg hkllnesslw vcpindspkp ppsritltlp
      181 vinnakkcif tvvgsskaei vkrilqdken lpaarvqphn gelywilded sskllqa
//
LOCUS       XP_034193466             211 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4E type 3-A isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034193466
VERSION     XP_034193466.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337575.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..211
                     /product="eukaryotic translation initiation factor 4E type
                     3-A isoform X1"
                     /calculated_mol_wt=23969
     Region          40..175
                     /region_name="IF4E"
                     /note="Eukaryotic initiation factor 4E; pfam01652"
                     /db_xref="CDD:460281"
     CDS             1..211
                     /gene="LOC117610301"
                     /coded_by="XM_034337575.2:29..664"
                     /db_xref="GeneID:117610301"
ORIGIN      
        1 masmesalkd eassgditqv pvfsderikr ieeqetsgdp lqsswtfwlg raisastvee
       61 yktnllkiyt vntvqgfwav fnnipsvkyi qvrwnyhlmr deryplweep vnqhggtwrl
      121 kchksdteki wkevvlaaig eqfdecvaeg devcgvtasv rdredvvdiw ntnaalaska
      181 tvlekvqsll pnvnctkfyk phqshhaygr s
//
LOCUS       XP_034193540             195 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4E type 3-A isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034193540
VERSION     XP_034193540.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337649.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..195
                     /product="eukaryotic translation initiation factor 4E type
                     3-A isoform X2"
                     /calculated_mol_wt=22267
     Region          40..>153
                     /region_name="IF4E"
                     /note="Eukaryotic initiation factor 4E; pfam01652"
                     /db_xref="CDD:460281"
     CDS             1..195
                     /gene="LOC117610301"
                     /coded_by="XM_034337649.2:31..618"
                     /db_xref="GeneID:117610301"
ORIGIN      
        1 masmesalkd eassgditqv pvfsderikr ieeqetsgdp lqsswtfwlg raisastvee
       61 yktnllkiyt vntvqgfwav fnnipsvkyi qvrwnyhlmr deryplweep vnqhggtwrl
      121 kchksdteki wkevvlaaig eqfdecvaeg deiwntnaal askatvlekv qsllpnvnct
      181 kfykphqshh aygrs
//
LOCUS       XP_076546160             904 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603072 [Osmia lignaria lignaria].
ACCESSION   XP_076546160
VERSION     XP_076546160.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690045.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..904
                     /product="uncharacterized protein LOC117603072"
                     /calculated_mol_wt=101856
     Region          178..229
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          237..288
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          293..345
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          351..>457
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          548..650
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     CDS             1..904
                     /gene="LOC117603072"
                     /coded_by="XM_076690045.1:89..2803"
                     /db_xref="GeneID:117603072"
ORIGIN      
        1 mhvqnisvki kcslilllyl ligeisntmq dldcdlglkn dnlcpidggw tswsswglct
       61 gkcgfkgkrt rhricnnplp sndgapcigp syqtencqii gcrmndyeev vsnhpirkke
      121 meivknvhkr lpalielcff vdctffivek ilgssamlyw namncvkynv gcprpggwst
      181 weawspctai cgrgqkyrtr ncnspvpsns elmcnnsafe vkscmglncq thpagtwinw
      241 sgwsacsvec gngiqvrkrs cseiqniqgs sckgsteeik gcsinkcsin giwsswtvwt
      301 actsscgigt qlrnrmcnnp spsgngtscs gsasevrqcf skpcsvrshe vayftekssl
      361 lynvngkhsr llhlyvrflp lspfgviiyr fennckgsmc dfvklflhng kiillskisg
      421 ctlglvhehk leigqwyvvl iaiygtrgml rindnrheia tfscipmsyn ldhamkvgef
      481 hgqlqeiain fapvqfhask dkydqkhant pfssnnvqyl mgdddegfly igltdsvaip
      541 spkglefwqi tlviktedin giiaiisdsf lnkcilllld egrvklkfhh gvnhvatesp
      601 ehilirewfe iivvqdgknl ymqingnekk yvpvmsegmi itsvpnvflg airdemreki
      661 cpscveipqm sftlgslnid gnqidllsla vfgttskrfs srtistsdyy eeiplllgqe
      721 lklscfynki pyekghlssk riyvvwllmd kllqsyesdd ffrlkddgrv stisivsyst
      781 reaienfysc hsyhrsklnk tsnqifftfg itaidkseef etvfwkewnf iyettivcfi
      841 ltilwcllei iqdakngygf yrlpqtpnnn ynsiikfvss ksgvtllgse qaaneilski
      901 ienn
//
LOCUS       XP_034174556             377 aa            linear   INV 03-SEP-2025
DEFINITION  Krueppel-like factor 6 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174556
VERSION     XP_034174556.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318665.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..377
                     /product="Krueppel-like factor 6 isoform X1"
                     /calculated_mol_wt=41948
     Region          2..>99
                     /region_name="KLF6_7_N-like"
                     /note="N-terminal domain of Kruppel-like factor (KLF) 6,
                     KLF7, and similar proteins; cd21973"
                     /db_xref="CDD:409246"
     Region          <283..>357
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          299..318
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(303,305,307,309..310,313..314,317,333,335,339..340,
                     343..344,347,361,363,365,367..368,371..372,375)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          326..348
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          340..365
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          354..376
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          356..376
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..377
                     /gene="LOC117601643"
                     /coded_by="XM_034318665.2:1140..2273"
                     /db_xref="GeneID:117601643"
ORIGIN      
        1 mdilpsgnif relqdihdtg yfsaqpsled hwqqtcyeme rylkdepklq sykklpteld
       61 tapwnlfrap piswtastgt anaqfdspik mevttssedr etlclddikf spgdhnhngr
      121 drdldrhlds lstsssssac sglswdssps vscrafilkk epsedleede eheeieeeed
      181 sgcesegqil tppsspgsgq ghsnsshsss gssmldvqnl nlhgtggrsa ivrvttsnaq
      241 gvarlisvta ngypavsgtq hahagttaaa ttvanrhhar shehsppdtk rrihkcqfpg
      301 ckkvytkssh lkahqrthtg ekpykcsweg cewrfarsde ltrhyrkhtg akpfkcrhcd
      361 rcfsrsdhla lhmkrhv
//
LOCUS       XP_076546161             339 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601643 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546161
VERSION     XP_076546161.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690046.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="uncharacterized protein LOC117601643 isoform X2"
                     /calculated_mol_wt=37480
     Region          <1..>61
                     /region_name="KLF6_7_N-like"
                     /note="N-terminal domain of Kruppel-like factor (KLF) 6,
                     KLF7, and similar proteins; cl41732"
                     /db_xref="CDD:425363"
     Region          <245..>319
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          261..280
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(265,267,269,271..272,275..276,279,295,297,301..302,
                     305..306,309,323,325,327,329..330,333..334,337)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          288..310
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          302..327
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          316..338
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          318..338
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..339
                     /gene="LOC117601643"
                     /coded_by="XM_076690046.1:1425..2444"
                     /db_xref="GeneID:117601643"
ORIGIN      
        1 merylkdepk lqsykklpte ldtapwnlfr appiswtast gtanaqfdsp ikmevttsse
       61 dretlclddi kfspgdhnhn grdrdldrhl dslstsssss acsglswdss psvscrafil
      121 kkepsedlee deeheeieee edsgcesegq iltppsspgs gqghsnsshs ssgssmldvq
      181 nlnlhgtggr saivrvttsn aqgvarlisv tangypavsg tqhahagtta aattvanrhh
      241 arshehsppd tkrrihkcqf pgckkvytks shlkahqrth tgekpykcsw egcewrfars
      301 deltrhyrkh tgakpfkcrh cdrcfsrsdh lalhmkrhv
//
LOCUS       XP_034174563             339 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601643 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174563
VERSION     XP_034174563.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318672.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="uncharacterized protein LOC117601643 isoform X2"
                     /calculated_mol_wt=37480
     Region          <1..>61
                     /region_name="KLF6_7_N-like"
                     /note="N-terminal domain of Kruppel-like factor (KLF) 6,
                     KLF7, and similar proteins; cl41732"
                     /db_xref="CDD:425363"
     Region          <245..>319
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          261..280
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(265,267,269,271..272,275..276,279,295,297,301..302,
                     305..306,309,323,325,327,329..330,333..334,337)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          288..310
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          302..327
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          316..338
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          318..338
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..339
                     /gene="LOC117601643"
                     /coded_by="XM_034318672.2:1400..2419"
                     /db_xref="GeneID:117601643"
ORIGIN      
        1 merylkdepk lqsykklpte ldtapwnlfr appiswtast gtanaqfdsp ikmevttsse
       61 dretlclddi kfspgdhnhn grdrdldrhl dslstsssss acsglswdss psvscrafil
      121 kkepsedlee deeheeieee edsgcesegq iltppsspgs gqghsnsshs ssgssmldvq
      181 nlnlhgtggr saivrvttsn aqgvarlisv tangypavsg tqhahagtta aattvanrhh
      241 arshehsppd tkrrihkcqf pgckkvytks shlkahqrth tgekpykcsw egcewrfars
      301 deltrhyrkh tgakpfkcrh cdrcfsrsdh lalhmkrhv
//
LOCUS       XP_076546162             339 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601643 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546162
VERSION     XP_076546162.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690047.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="uncharacterized protein LOC117601643 isoform X2"
                     /calculated_mol_wt=37480
     Region          <1..>61
                     /region_name="KLF6_7_N-like"
                     /note="N-terminal domain of Kruppel-like factor (KLF) 6,
                     KLF7, and similar proteins; cl41732"
                     /db_xref="CDD:425363"
     Region          <245..>319
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          261..280
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(265,267,269,271..272,275..276,279,295,297,301..302,
                     305..306,309,323,325,327,329..330,333..334,337)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          288..310
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          302..327
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          316..338
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          318..338
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..339
                     /gene="LOC117601643"
                     /coded_by="XM_076690047.1:1339..2358"
                     /db_xref="GeneID:117601643"
ORIGIN      
        1 merylkdepk lqsykklpte ldtapwnlfr appiswtast gtanaqfdsp ikmevttsse
       61 dretlclddi kfspgdhnhn grdrdldrhl dslstsssss acsglswdss psvscrafil
      121 kkepsedlee deeheeieee edsgcesegq iltppsspgs gqghsnsshs ssgssmldvq
      181 nlnlhgtggr saivrvttsn aqgvarlisv tangypavsg tqhahagtta aattvanrhh
      241 arshehsppd tkrrihkcqf pgckkvytks shlkahqrth tgekpykcsw egcewrfars
      301 deltrhyrkh tgakpfkcrh cdrcfsrsdh lalhmkrhv
//
LOCUS       XP_034171874             437 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600476 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171874
VERSION     XP_034171874.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315983.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..437
                     /product="uncharacterized protein LOC117600476 isoform X1"
                     /calculated_mol_wt=46895
     Region          197..253
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..437
                     /gene="LOC117600476"
                     /coded_by="XM_034315983.2:1770..3083"
                     /db_xref="GeneID:117600476"
ORIGIN      
        1 mpqrspfaiq ellglngtgn gsgtdrgspq gsppagvsav syaptaphhk lwdympsltn
       61 hlgnlgnlgn lgnltasrma ylnaqaavaa aflppphphp ahhvphhqvh qpgqlpaspa
      121 gvnvhpaaha qhvlpgtavt anhhqhsptq htpphgysqs kssfpnaspg vghniesgkd
      181 ftvdglsgfs kkkkkkrrhr tiftsqqlee leaafkeahy pdvyaremls lrtdlpedri
      241 qvwfqnrrak wrktekcwgr stimaeygly gamvrhslpl petilksake nesvapwlla
      301 qssktssfsq diennqemid cgpagmhrks ieaaehlksg gedsgndhng sgssphhnhh
      361 qggptssesg qesqdgmgps sstgmvqdte qlrnesiacl rakaqqhqlq lslqptpapt
      421 stsypagpps stlhasv
//
LOCUS       XP_034171894             412 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600476 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034171894
VERSION     XP_034171894.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..412
                     /product="uncharacterized protein LOC117600476 isoform X2"
                     /calculated_mol_wt=44225
     Region          197..253
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..412
                     /gene="LOC117600476"
                     /coded_by="XM_034316003.2:1770..3008"
                     /db_xref="GeneID:117600476"
ORIGIN      
        1 mpqrspfaiq ellglngtgn gsgtdrgspq gsppagvsav syaptaphhk lwdympsltn
       61 hlgnlgnlgn lgnltasrma ylnaqaavaa aflppphphp ahhvphhqvh qpgqlpaspa
      121 gvnvhpaaha qhvlpgtavt anhhqhsptq htpphgysqs kssfpnaspg vghniesgkd
      181 ftvdglsgfs kkkkkkrrhr tiftsqqlee leaafkeahy pdvyaremls lrtdlpedri
      241 qvwfqnrrak wrktekcwgr stimaeygly gamvrhslpl petilksake nesvapwllg
      301 mhrksieaae hlksggedsg ndhngsgssp hhnhhqggpt ssesgqesqd gmgpssstgm
      361 vqdteqlrne siaclrakaq qhqlqlslqp tpaptstsyp agppsstlha sv
//
LOCUS       XP_034175402            1335 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175402
VERSION     XP_034175402.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319511.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1335
                     /product="BAI1 associated protein 3 isoform X1"
                     /calculated_mol_wt=151344
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1116
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1162..1300
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1191,1197,1255,1257,1263)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1335
                     /gene="unc-13-4A"
                     /coded_by="XM_034319511.2:772..4779"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldkllaeh vvkhmentse mqnelgarvt taclqlhgqn
      781 gddeyvlppg aeigtpmfel ylalqdfifm kenlpqsdhk tlaickyyew frpavdkwld
      841 laklkaiqrv rksaeldric tgeyivkhct saidvtacfy qikefwkkla wpdlpgsynl
      901 vlkvvdaics saayyaeith qkladsgyye eqtpykttde viaqmcvain gleyvrrwll
      961 dlgeelhvek lltalecqag esarmqwrta lttpleqtpg qmllfinqiv srigtkmrpp
     1021 lkkamfhlaw spdslptsea iaplleyldv hlvalnsall vvnfnrvlqd iwevvlvels
     1081 nqmdgnagdk pamfyerlhe aldllikffh adekglpyda lhcqsflnve erlqyhkmdt
     1141 tflidrfyrq rlqdqlntva seygvltvra ylnhdslcve vinardvipl dpngfsdpfv
     1201 iiellprrvf ehcaeqrtnv kkktlnpmfd ecfefsvsve qcrspdamvl ftvmdrdvlt
     1261 addfageafl glstipgvad tnasidnfhg lkhtelplmq qknrshpilq iletrvgdkv
     1321 aldfvkkqkq rfast
//
LOCUS       XP_034175412            1335 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175412
VERSION     XP_034175412.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319521.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1335
                     /product="BAI1 associated protein 3 isoform X1"
                     /calculated_mol_wt=151344
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1116
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1162..1300
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1191,1197,1255,1257,1263)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1335
                     /gene="unc-13-4A"
                     /coded_by="XM_034319521.2:663..4670"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldkllaeh vvkhmentse mqnelgarvt taclqlhgqn
      781 gddeyvlppg aeigtpmfel ylalqdfifm kenlpqsdhk tlaickyyew frpavdkwld
      841 laklkaiqrv rksaeldric tgeyivkhct saidvtacfy qikefwkkla wpdlpgsynl
      901 vlkvvdaics saayyaeith qkladsgyye eqtpykttde viaqmcvain gleyvrrwll
      961 dlgeelhvek lltalecqag esarmqwrta lttpleqtpg qmllfinqiv srigtkmrpp
     1021 lkkamfhlaw spdslptsea iaplleyldv hlvalnsall vvnfnrvlqd iwevvlvels
     1081 nqmdgnagdk pamfyerlhe aldllikffh adekglpyda lhcqsflnve erlqyhkmdt
     1141 tflidrfyrq rlqdqlntva seygvltvra ylnhdslcve vinardvipl dpngfsdpfv
     1201 iiellprrvf ehcaeqrtnv kkktlnpmfd ecfefsvsve qcrspdamvl ftvmdrdvlt
     1261 addfageafl glstipgvad tnasidnfhg lkhtelplmq qknrshpilq iletrvgdkv
     1321 aldfvkkqkq rfast
//
LOCUS       XP_034175423            1335 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175423
VERSION     XP_034175423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1335
                     /product="BAI1 associated protein 3 isoform X1"
                     /calculated_mol_wt=151344
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1116
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1162..1300
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1191,1197,1255,1257,1263)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1335
                     /gene="unc-13-4A"
                     /coded_by="XM_034319532.2:748..4755"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldkllaeh vvkhmentse mqnelgarvt taclqlhgqn
      781 gddeyvlppg aeigtpmfel ylalqdfifm kenlpqsdhk tlaickyyew frpavdkwld
      841 laklkaiqrv rksaeldric tgeyivkhct saidvtacfy qikefwkkla wpdlpgsynl
      901 vlkvvdaics saayyaeith qkladsgyye eqtpykttde viaqmcvain gleyvrrwll
      961 dlgeelhvek lltalecqag esarmqwrta lttpleqtpg qmllfinqiv srigtkmrpp
     1021 lkkamfhlaw spdslptsea iaplleyldv hlvalnsall vvnfnrvlqd iwevvlvels
     1081 nqmdgnagdk pamfyerlhe aldllikffh adekglpyda lhcqsflnve erlqyhkmdt
     1141 tflidrfyrq rlqdqlntva seygvltvra ylnhdslcve vinardvipl dpngfsdpfv
     1201 iiellprrvf ehcaeqrtnv kkktlnpmfd ecfefsvsve qcrspdamvl ftvmdrdvlt
     1261 addfageafl glstipgvad tnasidnfhg lkhtelplmq qknrshpilq iletrvgdkv
     1321 aldfvkkqkq rfast
//
LOCUS       XP_034175436            1335 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175436
VERSION     XP_034175436.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319545.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1335
                     /product="BAI1 associated protein 3 isoform X1"
                     /calculated_mol_wt=151344
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1116
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1162..1300
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1191,1197,1255,1257,1263)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1335
                     /gene="unc-13-4A"
                     /coded_by="XM_034319545.2:663..4670"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldkllaeh vvkhmentse mqnelgarvt taclqlhgqn
      781 gddeyvlppg aeigtpmfel ylalqdfifm kenlpqsdhk tlaickyyew frpavdkwld
      841 laklkaiqrv rksaeldric tgeyivkhct saidvtacfy qikefwkkla wpdlpgsynl
      901 vlkvvdaics saayyaeith qkladsgyye eqtpykttde viaqmcvain gleyvrrwll
      961 dlgeelhvek lltalecqag esarmqwrta lttpleqtpg qmllfinqiv srigtkmrpp
     1021 lkkamfhlaw spdslptsea iaplleyldv hlvalnsall vvnfnrvlqd iwevvlvels
     1081 nqmdgnagdk pamfyerlhe aldllikffh adekglpyda lhcqsflnve erlqyhkmdt
     1141 tflidrfyrq rlqdqlntva seygvltvra ylnhdslcve vinardvipl dpngfsdpfv
     1201 iiellprrvf ehcaeqrtnv kkktlnpmfd ecfefsvsve qcrspdamvl ftvmdrdvlt
     1261 addfageafl glstipgvad tnasidnfhg lkhtelplmq qknrshpilq iletrvgdkv
     1321 aldfvkkqkq rfast
//
LOCUS       XP_034175426            1335 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175426
VERSION     XP_034175426.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319535.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1335
                     /product="BAI1 associated protein 3 isoform X1"
                     /calculated_mol_wt=151344
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1116
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1162..1300
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1191,1197,1255,1257,1263)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1335
                     /gene="unc-13-4A"
                     /coded_by="XM_034319535.2:723..4730"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldkllaeh vvkhmentse mqnelgarvt taclqlhgqn
      781 gddeyvlppg aeigtpmfel ylalqdfifm kenlpqsdhk tlaickyyew frpavdkwld
      841 laklkaiqrv rksaeldric tgeyivkhct saidvtacfy qikefwkkla wpdlpgsynl
      901 vlkvvdaics saayyaeith qkladsgyye eqtpykttde viaqmcvain gleyvrrwll
      961 dlgeelhvek lltalecqag esarmqwrta lttpleqtpg qmllfinqiv srigtkmrpp
     1021 lkkamfhlaw spdslptsea iaplleyldv hlvalnsall vvnfnrvlqd iwevvlvels
     1081 nqmdgnagdk pamfyerlhe aldllikffh adekglpyda lhcqsflnve erlqyhkmdt
     1141 tflidrfyrq rlqdqlntva seygvltvra ylnhdslcve vinardvipl dpngfsdpfv
     1201 iiellprrvf ehcaeqrtnv kkktlnpmfd ecfefsvsve qcrspdamvl ftvmdrdvlt
     1261 addfageafl glstipgvad tnasidnfhg lkhtelplmq qknrshpilq iletrvgdkv
     1321 aldfvkkqkq rfast
//
LOCUS       XP_034175445            1335 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175445
VERSION     XP_034175445.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319554.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1335
                     /product="BAI1 associated protein 3 isoform X1"
                     /calculated_mol_wt=151344
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1116
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1162..1300
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1191,1197,1255,1257,1263)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1335
                     /gene="unc-13-4A"
                     /coded_by="XM_034319554.2:768..4775"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldkllaeh vvkhmentse mqnelgarvt taclqlhgqn
      781 gddeyvlppg aeigtpmfel ylalqdfifm kenlpqsdhk tlaickyyew frpavdkwld
      841 laklkaiqrv rksaeldric tgeyivkhct saidvtacfy qikefwkkla wpdlpgsynl
      901 vlkvvdaics saayyaeith qkladsgyye eqtpykttde viaqmcvain gleyvrrwll
      961 dlgeelhvek lltalecqag esarmqwrta lttpleqtpg qmllfinqiv srigtkmrpp
     1021 lkkamfhlaw spdslptsea iaplleyldv hlvalnsall vvnfnrvlqd iwevvlvels
     1081 nqmdgnagdk pamfyerlhe aldllikffh adekglpyda lhcqsflnve erlqyhkmdt
     1141 tflidrfyrq rlqdqlntva seygvltvra ylnhdslcve vinardvipl dpngfsdpfv
     1201 iiellprrvf ehcaeqrtnv kkktlnpmfd ecfefsvsve qcrspdamvl ftvmdrdvlt
     1261 addfageafl glstipgvad tnasidnfhg lkhtelplmq qknrshpilq iletrvgdkv
     1321 aldfvkkqkq rfast
//
LOCUS       XP_034175454            1331 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034175454
VERSION     XP_034175454.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319563.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1331
                     /product="BAI1 associated protein 3 isoform X2"
                     /calculated_mol_wt=150933
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1112
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1158..1296
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1187,1193,1251,1253,1259)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1331
                     /gene="unc-13-4A"
                     /coded_by="XM_034319563.2:772..4767"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldkllaeh vvkhmentse mqnelgarvt taclqlhgqn
      781 gddeyvlppg aeigtpmfel ylalqdfifm kenlpqsdhk tlaickyyew frpavdkwld
      841 laklkaiqrv rksaeldric tgeyivkhct saidvtacfy qikefwkkla wpdlpgsynl
      901 vlkvvdaics saayyaeith qkladsgyye eqtpykttde mcvaingley vrrwlldlge
      961 elhvekllta lecqagesar mqwrtalttp leqtpgqmll finqivsrig tkmrpplkka
     1021 mfhlawspds lptseaiapl leyldvhlva lnsallvvnf nrvlqdiwev vlvelsnqmd
     1081 gnagdkpamf yerlhealdl likffhadek glpydalhcq sflnveerlq yhkmdttfli
     1141 drfyrqrlqd qlntvaseyg vltvraylnh dslcvevina rdvipldpng fsdpfviiel
     1201 lprrvfehca eqrtnvkkkt lnpmfdecfe fsvsveqcrs pdamvlftvm drdvltaddf
     1261 ageaflglst ipgvadtnas idnfhglkht elplmqqknr shpilqilet rvgdkvaldf
     1321 vkkqkqrfas t
//
LOCUS       XP_034175463            1321 aa            linear   INV 03-SEP-2025
DEFINITION  BAI1 associated protein 3 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034175463
VERSION     XP_034175463.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319572.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1321
                     /product="BAI1 associated protein 3 isoform X3"
                     /calculated_mol_wt=149738
     Region          240..478
                     /region_name="C2A_Munc13-like"
                     /note="C2 domain first repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd08676"
                     /db_xref="CDD:176058"
     Site            order(283,289,414,416)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176058"
     Region          <379..505
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          678..1102
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          1148..1286
                     /region_name="C2B_Munc13-like"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated)-like proteins; cd04009"
                     /db_xref="CDD:175976"
     Site            order(1177,1183,1241,1243,1249)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175976"
     CDS             1..1321
                     /gene="unc-13-4A"
                     /coded_by="XM_034319572.2:772..4737"
                     /db_xref="GeneID:117602040"
ORIGIN      
        1 msffnslqqy vtdsvaslsl spkrfsfsre dsttsgtgrs gstgsvtgan ntnstaqqst
       61 phgypkvvpp pgtvtsshsr slstrrrtle gtsspgltvh patgsasgst sprrvgsfrr
      121 stgsadrppl mfcrrrpswp evdiqatsgv qetdgsffes ftalswkqqn rrlvvlqeve
      181 arakepppps rdssltssqp yrfskkeleq lyievlytia ntvgastgqy ahykedlyry
      241 aqeafevpqd qhrrylnias eekppivvln vvvveaegle akdangfsdp ycmlgiqpgn
      301 tgaplspqtn ltahsphtpp asprqatral sdgaetensh heklrkhhsf rlsfkrkeht
      361 srrehresls gavpakfira tsvkphtlsp rwnekfrfdi ddintdilhl diwdhddess
      421 vfdavsklne vrgvkglgrf fkqiaqsars ggqddflgcv niplqdipst gldrwyklea
      481 rtqrstiqgr irlklrlstr edrgtseedn wgelrqqkrl ytvflnhemn kqgedftgel
      541 pqtaltilhq havqgdvtel qqalvkwvat srqptcdpri lhrllqeldn lwhtdtlsrd
      601 eeqcladsfn eflevtlkkv rqhrvlfppl hrssiskldy llkclgllsn mkafrtccpf
      661 nkeirgeiit alrkgtlewy edtrqqtmcy dkepdqdadk vlqdftnlvt allvdleqgl
      721 tyynslfest ngvpyfsavy kqldklmqne lgarvttacl qlhgqngdde yvlppgaeig
      781 tpmfelylal qdfifmkenl pqsdhktlai ckyyewfrpa vdkwldlakl kaiqrvrksa
      841 eldrictgey ivkhctsaid vtacfyqike fwkklawpdl pgsynlvlkv vdaicssaay
      901 yaeithqkla dsgyyeeqtp ykttdeviaq mcvaingley vrrwlldlge elhvekllta
      961 lecqagesar mqwrtalttp leqtpgqmll finqivsrig tkmrpplkka mfhlawspds
     1021 lptseaiapl leyldvhlva lnsallvvnf nrvlqdiwev vlvelsnqmd gnagdkpamf
     1081 yerlhealdl likffhadek glpydalhcq sflnveerlq yhkmdttfli drfyrqrlqd
     1141 qlntvaseyg vltvraylnh dslcvevina rdvipldpng fsdpfviiel lprrvfehca
     1201 eqrtnvkkkt lnpmfdecfe fsvsveqcrs pdamvlftvm drdvltaddf ageaflglst
     1261 ipgvadtnas idnfhglkht elplmqqknr shpilqilet rvgdkvaldf vkkqkqrfas
     1321 t
//
LOCUS       XP_034175480             697 aa            linear   INV 03-SEP-2025
DEFINITION  translation elongation factor EF-1alpha (GTPase) HBS1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034175480
VERSION     XP_034175480.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319589.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="translation elongation factor EF-1alpha (GTPase)
                     HBS1 isoform X1"
                     /calculated_mol_wt=77198
     Region          61..134
                     /region_name="HBS1_N"
                     /note="HBS1 N-terminus; pfam08938"
                     /db_xref="CDD:462642"
     Region          274..697
                     /region_name="TEF1"
                     /note="Translation elongation factor EF-1alpha (GTPase)
                     [Translation, ribosomal structure and biogenesis];
                     COG5256"
                     /db_xref="CDD:444074"
     CDS             1..697
                     /gene="HBS1"
                     /coded_by="XM_034319589.2:273..2366"
                     /db_xref="GeneID:117602072"
ORIGIN      
        1 msrhrdvrcm nyseeyenyd evyghsvedd ycvspsaeqf lfdrskqqni asfitepdil
       61 ednednresl spskeeervl teleraklis cmesikniig dtvpeteikk kiiqsnfdae
      121 valdlvlkes ppktviesst ghkdnlelys gnklsskptf qvtapsnvki lhagkrpavq
      181 gfdlsgtent dilnsrvesp rsrspysdhn speikrkert vpkekkavss nansprcqsp
      241 vpghvtpdfd skpklneekh dvlkiykdkr gdskehihlv vvghvdagks tllgrllcdl
      301 gqvpsrlihk yqqeskkigk qsfayawvld etgeerergi tmdighskfe tetksitlld
      361 apghkdfipn mitgatqadv allvvdatrg efetgfdsgg qtrehalllr slgvlqlavv
      421 inkldtvdws kerfneivek mnvflkqagf kdnvpfvpcs glsgenivtk pkeplsnwyt
      481 gptlvdvidn fkcperpink pfrfsvndif kgtgsgfcvs ghvetgmisl gdkvlilprn
      541 etavikglqv deasttnafa gdhvsltlsg idqqnvgigd iicipqnpvp vttcfeahiv
      601 vfavkipimk glpvvmhqqs lvqpavvtkl iaqihrttge tikkkprclp knssaiveir
      661 tqnpvcmely kdvkqlgrit lrvegttiaa glitkik
//
LOCUS       XP_034175490             697 aa            linear   INV 03-SEP-2025
DEFINITION  translation elongation factor EF-1alpha (GTPase) HBS1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034175490
VERSION     XP_034175490.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319599.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="translation elongation factor EF-1alpha (GTPase)
                     HBS1 isoform X1"
                     /calculated_mol_wt=77198
     Region          61..134
                     /region_name="HBS1_N"
                     /note="HBS1 N-terminus; pfam08938"
                     /db_xref="CDD:462642"
     Region          274..697
                     /region_name="TEF1"
                     /note="Translation elongation factor EF-1alpha (GTPase)
                     [Translation, ribosomal structure and biogenesis];
                     COG5256"
                     /db_xref="CDD:444074"
     CDS             1..697
                     /gene="HBS1"
                     /coded_by="XM_034319599.2:293..2386"
                     /db_xref="GeneID:117602072"
ORIGIN      
        1 msrhrdvrcm nyseeyenyd evyghsvedd ycvspsaeqf lfdrskqqni asfitepdil
       61 ednednresl spskeeervl teleraklis cmesikniig dtvpeteikk kiiqsnfdae
      121 valdlvlkes ppktviesst ghkdnlelys gnklsskptf qvtapsnvki lhagkrpavq
      181 gfdlsgtent dilnsrvesp rsrspysdhn speikrkert vpkekkavss nansprcqsp
      241 vpghvtpdfd skpklneekh dvlkiykdkr gdskehihlv vvghvdagks tllgrllcdl
      301 gqvpsrlihk yqqeskkigk qsfayawvld etgeerergi tmdighskfe tetksitlld
      361 apghkdfipn mitgatqadv allvvdatrg efetgfdsgg qtrehalllr slgvlqlavv
      421 inkldtvdws kerfneivek mnvflkqagf kdnvpfvpcs glsgenivtk pkeplsnwyt
      481 gptlvdvidn fkcperpink pfrfsvndif kgtgsgfcvs ghvetgmisl gdkvlilprn
      541 etavikglqv deasttnafa gdhvsltlsg idqqnvgigd iicipqnpvp vttcfeahiv
      601 vfavkipimk glpvvmhqqs lvqpavvtkl iaqihrttge tikkkprclp knssaiveir
      661 tqnpvcmely kdvkqlgrit lrvegttiaa glitkik
//
LOCUS       XP_034175498             683 aa            linear   INV 03-SEP-2025
DEFINITION  translation elongation factor EF-1alpha (GTPase) HBS1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034175498
VERSION     XP_034175498.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319607.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..683
                     /product="translation elongation factor EF-1alpha (GTPase)
                     HBS1 isoform X2"
                     /calculated_mol_wt=75637
     Region          61..134
                     /region_name="HBS1_N"
                     /note="HBS1 N-terminus; pfam08938"
                     /db_xref="CDD:462642"
     Region          260..683
                     /region_name="TEF1"
                     /note="Translation elongation factor EF-1alpha (GTPase)
                     [Translation, ribosomal structure and biogenesis];
                     COG5256"
                     /db_xref="CDD:444074"
     CDS             1..683
                     /gene="HBS1"
                     /coded_by="XM_034319607.2:274..2325"
                     /db_xref="GeneID:117602072"
ORIGIN      
        1 msrhrdvrcm nyseeyenyd evyghsvedd ycvspsaeqf lfdrskqqni asfitepdil
       61 ednednresl spskeeervl teleraklis cmesikniig dtvpeteikk kiiqsnfdae
      121 valdlvlkes ppktvignkl sskptfqvta psnvkilhag krpavqgfdl sgtentdiln
      181 srvesprsrs pysdhnspei krkertvpke kkavssnans prcqspvpgh vtpdfdskpk
      241 lneekhdvlk iykdkrgdsk ehihlvvvgh vdagkstllg rllcdlgqvp srlihkyqqe
      301 skkigkqsfa yawvldetge erergitmdi ghskfetetk sitlldapgh kdfipnmitg
      361 atqadvallv vdatrgefet gfdsggqtre halllrslgv lqlavvinkl dtvdwskerf
      421 neivekmnvf lkqagfkdnv pfvpcsglsg enivtkpkep lsnwytgptl vdvidnfkcp
      481 erpinkpfrf svndifkgtg sgfcvsghve tgmislgdkv lilprnetav ikglqvdeas
      541 ttnafagdhv sltlsgidqq nvgigdiici pqnpvpvttc feahivvfav kipimkglpv
      601 vmhqqslvqp avvtkliaqi hrttgetikk kprclpknss aiveirtqnp vcmelykdvk
      661 qlgritlrve gttiaaglit kik
//
LOCUS       XP_034175508             729 aa            linear   INV 03-SEP-2025
DEFINITION  translation elongation factor EF-1alpha (GTPase) HBS1 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034175508
VERSION     XP_034175508.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319617.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="translation elongation factor EF-1alpha (GTPase)
                     HBS1 isoform X3"
                     /calculated_mol_wt=80840
     Region          61..134
                     /region_name="HBS1_N"
                     /note="HBS1 N-terminus; pfam08938"
                     /db_xref="CDD:462642"
     Region          306..729
                     /region_name="TEF1"
                     /note="Translation elongation factor EF-1alpha (GTPase)
                     [Translation, ribosomal structure and biogenesis];
                     COG5256"
                     /db_xref="CDD:444074"
     CDS             1..729
                     /gene="HBS1"
                     /coded_by="XM_034319617.2:274..2463"
                     /db_xref="GeneID:117602072"
ORIGIN      
        1 msrhrdvrcm nyseeyenyd evyghsvedd ycvspsaeqf lfdrskqqni asfitepdil
       61 ednednresl spskeeervl teleraklis cmesikniig dtvpeteikk kiiqsnfdae
      121 valdlvlkes ppktviesst ghkdnlelys datpiklqst sfvipkfsfn kqnniqnsnm
      181 nscnklsskp tfqvtapsnv kilhagkrpa vqgfdlsgte ntdilnsrve sprsrspysd
      241 hnspeikrke rtvpkekkav ssnansprcq spvpghvtpd fdskpklnee khdvlkiykd
      301 krgdskehih lvvvghvdag kstllgrllc dlgqvpsrli hkyqqeskki gkqsfayawv
      361 ldetgeerer gitmdighsk fetetksitl ldapghkdfi pnmitgatqa dvallvvdat
      421 rgefetgfds ggqtrehall lrslgvlqla vvinkldtvd wskerfneiv ekmnvflkqa
      481 gfkdnvpfvp csglsgeniv tkpkeplsnw ytgptlvdvi dnfkcperpi nkpfrfsvnd
      541 ifkgtgsgfc vsghvetgmi slgdkvlilp rnetavikgl qvdeasttna fagdhvsltl
      601 sgidqqnvgi gdiicipqnp vpvttcfeah ivvfavkipi mkglpvvmhq qslvqpavvt
      661 kliaqihrtt getikkkprc lpknssaive irtqnpvcme lykdvkqlgr itlrvegtti
      721 aaglitkik
//
LOCUS       XP_034175530             622 aa            linear   INV 03-SEP-2025
DEFINITION  cilia- and flagella-associated protein 99 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175530
VERSION     XP_034175530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319639.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..622
                     /product="cilia- and flagella-associated protein 99"
                     /calculated_mol_wt=73305
     Region          <365..614
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     CDS             1..622
                     /gene="LOC117602095"
                     /coded_by="XM_034319639.2:1775..3643"
                     /db_xref="GeneID:117602095"
ORIGIN      
        1 mncteeeltk rnvfhlniln plikifesym iikkelspde fclmlfakke ekercendyd
       61 llrerfdnle dlkvlkdlsi seqniiadlf inivryesvl eaiskdflnh lhyrnnnely
      121 qtmvlvytls yrisnlffee sidcfvhfki kkilslvlyl lrsetsnlyy ekgrlifeed
      181 fveqklvlpf leitsclekl rdqlkeylrk mkkpkpklti ptfmnvlnhp rhptrapppt
      241 pedlsamrfa rdvprtnyvk pstemrlesl rvanklqamn llkdanenap sclkrckvfv
      301 ksteeekpka tfvrksaptf kpvvvkhtaa stlrecarvi gneekeirkl kglleggldp
      361 saiskfeeek rqqqkeeell kiqekhllgl lthegafiak qsllndiksh aesvrkekqe
      421 lyeklekwre dhnkqmieiv ekcreveqss reafnamide krqkaaevse esrqlklqmi
      481 kqreeetqrk ikliqeiktl qslralpfkd fdptessglg lfcemslvel kerlfwtkmk
      541 lneeiqnrks airqqrdrqk nlikdtrral eeykankqaa psmkselkev sspeiealrk
      601 mleerralry qknnvcnssf nk
//
LOCUS       XP_034175543             521 aa            linear   INV 03-SEP-2025
DEFINITION  proton-coupled amino acid transporter 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175543
VERSION     XP_034175543.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319652.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..521
                     /product="proton-coupled amino acid transporter 2 isoform
                     X1"
                     /calculated_mol_wt=57498
     Region          98..509
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..521
                     /gene="LOC117602101"
                     /coded_by="XM_034319652.2:1..1566"
                     /db_xref="GeneID:117602101"
ORIGIN      
        1 mihcgmcnkr dgiasfllpl sfslfittne eghefsmemh kntnskkssi rademtlkep
       61 hrngstygaf qskdpilave kgseiesvhn ehgisvhhpt syletmmhlf kgnvgsgifa
      121 lgdafkhagl llappltifl gvicvhaqhi likcneevtr rvddvsastg fagtvelcfa
      181 tgplalrkys vfmrqlvnvf lcitqlgfcc vyfvfiatnm kqvldvygie mdvhqhmavi
      241 lipimlstwi rnlkylvpis smanflvtag yiatmyimfh dlppvqerry iadwhdlplf
      301 fgtviysfeg itlvlplkne mkkpsnfnks fgvlnvgmvi vgamfvamgf isylkygdav
      361 agsvtlnles tevvdgkiiv thsslpqcik vaislsillt yalqfyvpia iiwpkivnqf
      421 gpfqwpviae tvfrsavcfl tfilaeaipq lglfislvga vsstalalvf ppiiemvvcw
      481 qnaslspfti skdilivlig llgfvtgtye slnsiirafs t
//
LOCUS       XP_034175552             521 aa            linear   INV 03-SEP-2025
DEFINITION  proton-coupled amino acid transporter 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175552
VERSION     XP_034175552.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319661.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..521
                     /product="proton-coupled amino acid transporter 2 isoform
                     X2"
                     /calculated_mol_wt=57731
     Region          98..509
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..521
                     /gene="LOC117602101"
                     /coded_by="XM_034319661.2:16..1581"
                     /db_xref="GeneID:117602101"
ORIGIN      
        1 mylplnslrp rlrlfageek avtswfenli rlcnvememh kntnskkssi rademtlkep
       61 hrngstygaf qskdpilave kgseiesvhn ehgisvhhpt syletmmhlf kgnvgsgifa
      121 lgdafkhagl llappltifl gvicvhaqhi likcneevtr rvddvsastg fagtvelcfa
      181 tgplalrkys vfmrqlvnvf lcitqlgfcc vyfvfiatnm kqvldvygie mdvhqhmavi
      241 lipimlstwi rnlkylvpis smanflvtag yiatmyimfh dlppvqerry iadwhdlplf
      301 fgtviysfeg itlvlplkne mkkpsnfnks fgvlnvgmvi vgamfvamgf isylkygdav
      361 agsvtlnles tevvdgkiiv thsslpqcik vaislsillt yalqfyvpia iiwpkivnqf
      421 gpfqwpviae tvfrsavcfl tfilaeaipq lglfislvga vsstalalvf ppiiemvvcw
      481 qnaslspfti skdilivlig llgfvtgtye slnsiirafs t
//
LOCUS       XP_034175562             510 aa            linear   INV 03-SEP-2025
DEFINITION  proton-coupled amino acid transporter 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175562
VERSION     XP_034175562.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319671.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="proton-coupled amino acid transporter 2 isoform
                     X3"
                     /calculated_mol_wt=56361
     Region          98..498
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..510
                     /gene="LOC117602101"
                     /coded_by="XM_034319671.2:1..1533"
                     /db_xref="GeneID:117602101"
ORIGIN      
        1 mihcgmcnkr dgiasfllpl sfslfittne eghefsmemh kntnskkssi rademtlkep
       61 hrngstygaf qskdpilave kgseiesvhn ehgisvhhpt syletmmhlf kgnvgsgifa
      121 lgdafkhagl llappltifl gvicvhaqhi likcneevtr rvddvsastg fagtvelcfa
      181 tgplalrkys vfmrqlvnvf lcitqlgfcc vyfvfiatnm kqvldvygie mdvhqhmavi
      241 lipimlstwi rnlkylvpis smanflvtag yiatmyimfh dlppvqerry iadwhdlplf
      301 fgtviysfeg itlvlplkne mkkpsnfnks fgvlnvgmvi vgamfvamgf isylkygdav
      361 agsvtlnles tevlpqcikv aislsillty alqfyvpiai iwpkivnqfg pfqwpviaet
      421 vfrsavcflt filaeaipql glfislvgav sstalalvfp piiemvvcwq naslspftis
      481 kdilivligl lgfvtgtyes lnsiirafst
//
LOCUS       XP_076546163             485 aa            linear   INV 03-SEP-2025
DEFINITION  proton-coupled amino acid transporter 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546163
VERSION     XP_076546163.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690048.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="proton-coupled amino acid transporter 2 isoform
                     X4"
                     /calculated_mol_wt=53469
     Region          62..473
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..485
                     /gene="LOC117602101"
                     /coded_by="XM_076690048.1:2856..4313"
                     /db_xref="GeneID:117602101"
ORIGIN      
        1 memhkntnsk kssirademt lkephrngst ygafqskdpi lavekgseie svhnehgisv
       61 hhptsyletm mhlfkgnvgs gifalgdafk haglllappl tiflgvicvh aqhilikcne
      121 evtrrvddvs astgfagtve lcfatgplal rkysvfmrql vnvflcitql gfccvyfvfi
      181 atnmkqvldv ygiemdvhqh mavilipiml stwirnlkyl vpissmanfl vtagyiatmy
      241 imfhdlppvq erryiadwhd lplffgtviy sfegitlvlp lknemkkpsn fnksfgvlnv
      301 gmvivgamfv amgfisylky gdavagsvtl nlestevvdg kiivthsslp qcikvaisls
      361 illtyalqfy vpiaiiwpki vnqfgpfqwp viaetvfrsa vcfltfilae aipqlglfis
      421 lvgavsstal alvfppiiem vvcwqnasls pftiskdili vligllgfvt gtyeslnsii
      481 rafst
//
LOCUS       XP_076546164             485 aa            linear   INV 03-SEP-2025
DEFINITION  proton-coupled amino acid transporter 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546164
VERSION     XP_076546164.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690049.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="proton-coupled amino acid transporter 2 isoform
                     X4"
                     /calculated_mol_wt=53469
     Region          62..473
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..485
                     /gene="LOC117602101"
                     /coded_by="XM_076690049.1:2847..4304"
                     /db_xref="GeneID:117602101"
ORIGIN      
        1 memhkntnsk kssirademt lkephrngst ygafqskdpi lavekgseie svhnehgisv
       61 hhptsyletm mhlfkgnvgs gifalgdafk haglllappl tiflgvicvh aqhilikcne
      121 evtrrvddvs astgfagtve lcfatgplal rkysvfmrql vnvflcitql gfccvyfvfi
      181 atnmkqvldv ygiemdvhqh mavilipiml stwirnlkyl vpissmanfl vtagyiatmy
      241 imfhdlppvq erryiadwhd lplffgtviy sfegitlvlp lknemkkpsn fnksfgvlnv
      301 gmvivgamfv amgfisylky gdavagsvtl nlestevvdg kiivthsslp qcikvaisls
      361 illtyalqfy vpiaiiwpki vnqfgpfqwp viaetvfrsa vcfltfilae aipqlglfis
      421 lvgavsstal alvfppiiem vvcwqnasls pftiskdili vligllgfvt gtyeslnsii
      481 rafst
//
LOCUS       XP_076546165             485 aa            linear   INV 03-SEP-2025
DEFINITION  proton-coupled amino acid transporter 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546165
VERSION     XP_076546165.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690050.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="proton-coupled amino acid transporter 2 isoform
                     X4"
                     /calculated_mol_wt=53469
     Region          62..473
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..485
                     /gene="LOC117602101"
                     /coded_by="XM_076690050.1:2791..4248"
                     /db_xref="GeneID:117602101"
ORIGIN      
        1 memhkntnsk kssirademt lkephrngst ygafqskdpi lavekgseie svhnehgisv
       61 hhptsyletm mhlfkgnvgs gifalgdafk haglllappl tiflgvicvh aqhilikcne
      121 evtrrvddvs astgfagtve lcfatgplal rkysvfmrql vnvflcitql gfccvyfvfi
      181 atnmkqvldv ygiemdvhqh mavilipiml stwirnlkyl vpissmanfl vtagyiatmy
      241 imfhdlppvq erryiadwhd lplffgtviy sfegitlvlp lknemkkpsn fnksfgvlnv
      301 gmvivgamfv amgfisylky gdavagsvtl nlestevvdg kiivthsslp qcikvaisls
      361 illtyalqfy vpiaiiwpki vnqfgpfqwp viaetvfrsa vcfltfilae aipqlglfis
      421 lvgavsstal alvfppiiem vvcwqnasls pftiskdili vligllgfvt gtyeslnsii
      481 rafst
//
LOCUS       XP_034175575             480 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic non-specific dipeptidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034175575
VERSION     XP_034175575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..480
                     /product="cytosolic non-specific dipeptidase 2"
                     /calculated_mol_wt=53177
     Region          12..479
                     /region_name="M20_dipept_like_CNDP"
                     /note="M20 cytosolic nonspecific dipeptidases including
                     anserinase and serum carnosinase; cd05676"
                     /db_xref="CDD:349925"
     Site            order(104,137,171..172,200,202,215,349,386,420,422..424,
                     451)
                     /site_type="active"
                     /db_xref="CDD:349925"
     Site            order(108,114,216,218,220,222,228..231,233..243,245,249,
                     252..253,256..258,292,295..297,300..301,310,313..314,
                     319..326,330..337,339..340,345,349,374..375,377,386,
                     421..422,450..453)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349925"
     CDS             1..480
                     /gene="Cndp2"
                     /coded_by="XM_034319684.2:211..1653"
                     /db_xref="GeneID:117602111"
ORIGIN      
        1 maselpptlk nlfsyidnhk veyinnlkea vaiksvsawp dhrneitkmi kwaevrlknl
       61 gatteladig kqtlpdgtqi plppillgtl gsnpkkrtvl lyghldvqpa lktdgwdtep
      121 ftlvekdgkl fgrgstddkg pvlcwihalq aykaigvdip vnlkfvfegm eesgseglde
      181 llwarkdtfl qgvdyvcisd nywlgttkpc ityglrgicy fnveitcadk dlhsgtfggi
      241 vyeavpdliy llntlvdvng kilidgmyds vakvteqele sykkiefdva dlkqsvgakk
      301 lahnedkiql lmhrwrqpsl slhgiegafs epgaktvipk kvigkfsiri vpnmtpeetm
      361 ekvkahinkh wavrgspntm nvimghsgks wtenpdhphy magrkatkhv ykvdpdlsre
      421 ggsipvtltf qevtgknvll lpvgagddga hsqneklnir nyiegtkllg aylyevsqla
//
LOCUS       XP_076546166             480 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic non-specific dipeptidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_076546166
VERSION     XP_076546166.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690051.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..480
                     /product="cytosolic non-specific dipeptidase 2"
                     /calculated_mol_wt=53177
     Region          12..479
                     /region_name="M20_dipept_like_CNDP"
                     /note="M20 cytosolic nonspecific dipeptidases including
                     anserinase and serum carnosinase; cd05676"
                     /db_xref="CDD:349925"
     Site            order(104,137,171..172,200,202,215,349,386,420,422..424,
                     451)
                     /site_type="active"
                     /db_xref="CDD:349925"
     Site            order(108,114,216,218,220,222,228..231,233..243,245,249,
                     252..253,256..258,292,295..297,300..301,310,313..314,
                     319..326,330..337,339..340,345,349,374..375,377,386,
                     421..422,450..453)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349925"
     CDS             1..480
                     /gene="Cndp2"
                     /coded_by="XM_076690051.1:91..1533"
                     /db_xref="GeneID:117602111"
ORIGIN      
        1 maselpptlk nlfsyidnhk veyinnlkea vaiksvsawp dhrneitkmi kwaevrlknl
       61 gatteladig kqtlpdgtqi plppillgtl gsnpkkrtvl lyghldvqpa lktdgwdtep
      121 ftlvekdgkl fgrgstddkg pvlcwihalq aykaigvdip vnlkfvfegm eesgseglde
      181 llwarkdtfl qgvdyvcisd nywlgttkpc ityglrgicy fnveitcadk dlhsgtfggi
      241 vyeavpdliy llntlvdvng kilidgmyds vakvteqele sykkiefdva dlkqsvgakk
      301 lahnedkiql lmhrwrqpsl slhgiegafs epgaktvipk kvigkfsiri vpnmtpeetm
      361 ekvkahinkh wavrgspntm nvimghsgks wtenpdhphy magrkatkhv ykvdpdlsre
      421 ggsipvtltf qevtgknvll lpvgagddga hsqneklnir nyiegtkllg aylyevsqla
//
LOCUS       XP_034175588             477 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-L-fucosidase [Osmia lignaria lignaria].
ACCESSION   XP_034175588
VERSION     XP_034175588.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319697.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="alpha-L-fucosidase"
                     /calculated_mol_wt=55410
     Region          47..429
                     /region_name="Alpha_L_fucos"
                     /note="Alpha-L-fucosidase; smart00812"
                     /db_xref="CDD:214829"
     Region          392..475
                     /region_name="Fucosidase_C"
                     /note="Alpha-L-fucosidase C-terminal domain; pfam16757"
                     /db_xref="CDD:465259"
     CDS             1..477
                     /gene="Fuca"
                     /coded_by="XM_034319697.2:151..1584"
                     /db_xref="GeneID:117602118"
ORIGIN      
        1 mivnlpifil lsssilympg kseyihisvs sdkekyetlw pletevgeys ptwnsldsrp
       61 lpqwyddakf gifihwgvfs vpsfgsewfw nnwkeehdst kyrdfmkqry ppnftyqdfa
      121 hdftaeffna tqwselfqas gakyvvltsk hhegytlwps kysfswnsvd vgpqkdlige
      181 lssairsstn lkfglyhsmy ewynplylsd ketnftteif vqqkiipelh elvekykpev
      241 vwsdgdwdap dtywkskefl awlynespvk dtvvvndrwg anipchhgdf ytcsdrynpg
      301 vllphkwenc mtidrkswgf rrnavlseyf tlpelvkelv vtvscggnll mnvgptkdgi
      361 ispifeerlr gmgdwlking eaiyntkpwi aqndtlnnnv wytctkdkkq iyaiilewps
      421 ndtlhlgslv vtdvsqisll gsknsiewqq dkgklnvflp aelnkgqpaw vlkieqi
//
LOCUS       XP_034175603             430 aa            linear   INV 03-SEP-2025
DEFINITION  rad, Gem/Kir family member 3 [Osmia lignaria lignaria].
ACCESSION   XP_034175603
VERSION     XP_034175603.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319712.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="rad, Gem/Kir family member 3"
                     /calculated_mol_wt=48004
     Region          213..430
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            219..226
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(221..227,271,322..323,325,352..354)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            243
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            247..249
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            268..271
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(270..271,282..283)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            322..325
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            352..354
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..430
                     /gene="Rgk3"
                     /coded_by="XM_034319712.2:649..1941"
                     /db_xref="GeneID:117602122"
ORIGIN      
        1 maenslrrkt rsasicapgf ssmeqmleam ppsgarerdk idrekdsgsg tpdsstptrr
       61 rrpatrsqsa rvsganksir rraaaqaahh hhhhhegtvk sahcsseprl tdndispglr
      121 rrgsrrgqsm hhthhrksna fldvpdtssq mpareegede dsyrlrsfsl tskgvvnrgd
      181 sfrrrrsrsn slapadpene enrsappkev ssytvamlga rgvgktalis qfmtsecina
      241 ydrqrdvpse qsvfimlnge eselrflnia npkseleslh pdafiimysv idkasfqrae
      301 eylerlhdqd llqgkpailv gnkvdlvrsk vvssqdgkcm actyrvkfie isvginhnvd
      361 nllvgilnqi rlknvqgnen rtgngtsegs ghwyksrgvv rasmkarqml twlfgkedsk
      421 fkncenlhvl
//
LOCUS       XP_034175641             317 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602139 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175641
VERSION     XP_034175641.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319750.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="uncharacterized protein LOC117602139 isoform X1"
                     /calculated_mol_wt=35676
     CDS             1..317
                     /gene="LOC117602139"
                     /coded_by="XM_034319750.2:176..1129"
                     /db_xref="GeneID:117602139"
ORIGIN      
        1 mnaelkkkat ieegfchdda dcsaqqycyr vsercvdytq csrynrqenr dkrsrhpsqc
       61 gpcfsgytae elgtgemawv ckkaniqndv pdvgklnint iiiwttisgv lfvsllvmlv
      121 glyvrrrask rrnskpeqcg dlsmmepsap pvenspfish gkelqhipfn nnknfkdnnn
      181 lvcasgfkpp swirtnpnyd nnlnnenmtt tgqlqsvvei tptdvqsntw qqdqltveit
      241 ngnliqyeve qvdnntntvl lqtnnpsssn tndeednnnt snnsdssnah edrervrtsn
      301 ilispkismn vnllnid
//
LOCUS       XP_034175656             310 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602139 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175656
VERSION     XP_034175656.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319765.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="uncharacterized protein LOC117602139 isoform X2"
                     /calculated_mol_wt=34864
     CDS             1..310
                     /gene="LOC117602139"
                     /coded_by="XM_034319765.2:168..1100"
                     /db_xref="GeneID:117602139"
ORIGIN      
        1 matieegfch ddadcsaqqy cyrvsercvd ytqcsrynrq enrdkrsrhp sqcgpcfsgy
       61 taeelgtgem awvckkaniq ndvpdvgkln intiiiwtti sgvlfvsllv mlvglyvrrr
      121 askrrnskpe qcgdlsmmep sappvenspf ishgkelqhi pfnnnknfkd nnnlvcasgf
      181 kppswirtnp nydnnlnnen mtttgqlqsv veitptdvqs ntwqqdqltv eitngnliqy
      241 eveqvdnntn tvllqtnnps ssntndeedn nntsnnsdss nahedrervr tsnilispki
      301 smnvnllnid
//
LOCUS       XP_034175670             311 aa            linear   INV 03-SEP-2025
DEFINITION  THUMP domain-containing protein 1 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034175670
VERSION     XP_034175670.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319779.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175670.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="THUMP domain-containing protein 1 homolog"
                     /calculated_mol_wt=35809
     Region          113..252
                     /region_name="THUMP_THUMPD1_like"
                     /note="THUMP domain-containing protein 1-like; cd11717"
                     /db_xref="CDD:212586"
     CDS             1..311
                     /gene="LOC117602148"
                     /coded_by="XM_034319779.2:206..1141"
                     /db_xref="GeneID:117602148"
ORIGIN      
        1 mdiqkrkrsf ynnnkkkhkq fnlepgmigf lctcnyrekd cirdayklln efadeiygsn
       61 ttkdsnndnm egseikkqds vsdteksdee distalnkei kelkteyakp vdfrrfqivd
      121 tgvknvifvr ssltnplelv tkiiteldkt kqqrtrfllr llpievvcka ymtdiklkad
      181 amlekyfaqe pktfsivfnr hsnnninreq viedlaglis kknpgnkadl knpeiavvve
      241 virgvclmsi aphyykfkky nlleicnike padnakksnt deteeskmnk dtkedtdlsq
      301 katddnkese n
//
LOCUS       XP_034175695             235 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175695
VERSION     XP_034175695.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319804.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175695.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="ubiquitin carboxyl-terminal hydrolase isoform
                     X1"
                     /calculated_mol_wt=26178
     Region          4..227
                     /region_name="Peptidase_C12_UCH_L1_L3"
                     /note="Cysteine peptidase C12 containing ubiquitin
                     carboxyl-terminal hydrolase (UCH) families L1 and L3;
                     cd09616"
                     /db_xref="CDD:187737"
     Site            order(6..11,31..34,53,96,168,170,213)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:187737"
     Site            order(6,90,94,96,168..169)
                     /site_type="inhibition"
                     /note="inhibitor binding site [chemical binding]"
                     /db_xref="CDD:187737"
     Site            order(90,96,169,184)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:187737"
     CDS             1..235
                     /gene="Uch"
                     /coded_by="XM_034319804.2:148..855"
                     /db_xref="GeneID:117602164"
ORIGIN      
        1 mmswiplesn pdvmttflhk lgvpkewsii dvyglepdll alvpkpvlav illyplrkkt
       61 gngleseedv kesdtstpsl kdpsvyhmkq yihnacgtia lihsignnld rinlqdgvly
      121 kflneskdls fsergellmk sqdignthee lahegqtevp sqetpiyhhf iafiqrngvl
      181 yeldgrksgp iehgatspet lledaarvck eymardpeev cftvvalvns daeaf
//
LOCUS       XP_034175705             194 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175705
VERSION     XP_034175705.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319814.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175705.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..194
                     /product="ubiquitin carboxyl-terminal hydrolase isoform
                     X2"
                     /calculated_mol_wt=21903
     Region          4..183
                     /region_name="Peptidase_C12_UCH_L1_L3"
                     /note="Cysteine peptidase C12 containing ubiquitin
                     carboxyl-terminal hydrolase (UCH) families L1 and L3;
                     cd09616"
                     /db_xref="CDD:187737"
     Site            order(6..11,31..34,53,96,168,170)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:187737"
     Site            order(6,90,94,96,168..169)
                     /site_type="inhibition"
                     /note="inhibitor binding site [chemical binding]"
                     /db_xref="CDD:187737"
     CDS             1..194
                     /gene="Uch"
                     /coded_by="XM_034319814.2:153..737"
                     /db_xref="GeneID:117602164"
ORIGIN      
        1 mmswiplesn pdvmttflhk lgvpkewsii dvyglepdll alvpkpvlav illyplrkkt
       61 gngleseedv kesdtstpsl kdpsvyhmkq yihnacgtia lihsignnld rinlqdgvly
      121 kflneskdls fsergellmk sqdignthee lahegqtevp sqetpiyhhf iafiqrngvl
      181 yelgenlyvk eiwt
//
LOCUS       XP_034175715             207 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal light chain 1 [Osmia lignaria lignaria].
ACCESSION   XP_034175715
VERSION     XP_034175715.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319824.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..207
                     /product="dynein axonemal light chain 1"
                     /calculated_mol_wt=23674
     Region          39..186
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          52..73
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          74..96
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          97..118
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          119..140
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..173
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275382"
     CDS             1..207
                     /gene="LOC117602170"
                     /coded_by="XM_034319824.2:1..624"
                     /db_xref="GeneID:117602170"
ORIGIN      
        1 msnvkpttck eairrweeee geeastakev ilsfqwppie kmdnalttlv ncerlslstn
       61 miekiagiga lknltilsvg rnlikgfsgl ealgdtlqel wisynliekm kginsmrnlr
      121 vlymsnnlvr ewnefnrlqe laylqnllfv gnplyesmev eqwrsevarr lpflekldge
      181 piirteesvi cinqsqmids psqslfe
//
LOCUS       XP_034175739             198 aa            linear   INV 03-SEP-2025
DEFINITION  histone chaperone asf1 [Osmia lignaria lignaria].
ACCESSION   XP_034175739
VERSION     XP_034175739.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319848.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..198
                     /product="histone chaperone asf1"
                     /calculated_mol_wt=22265
     Region          1..154
                     /region_name="ASF1_hist_chap"
                     /note="ASF1 like histone chaperone; pfam04729"
                     /db_xref="CDD:461412"
     CDS             1..198
                     /gene="asf1"
                     /coded_by="XM_034319848.2:268..864"
                     /db_xref="GeneID:117602176"
ORIGIN      
        1 makvqlanva vldnpspfln pfqfevtfec ieelkedlew kmiyvgsaes eefdqvldti
       61 yvgpipegrh mfvfqadppd vsripvndal gvtvvlltcs yrghefvrvg yfinneytda
      121 elrenpppqp qfdkvqrnil gdkprvtrfk inwddsasst tnnvpegmdp qsleetsqda
      181 ptstlgftes thnsmevm
//
LOCUS       XP_034175744             198 aa            linear   INV 03-SEP-2025
DEFINITION  histone chaperone asf1 [Osmia lignaria lignaria].
ACCESSION   XP_034175744
VERSION     XP_034175744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..198
                     /product="histone chaperone asf1"
                     /calculated_mol_wt=22265
     Region          1..154
                     /region_name="ASF1_hist_chap"
                     /note="ASF1 like histone chaperone; pfam04729"
                     /db_xref="CDD:461412"
     CDS             1..198
                     /gene="asf1"
                     /coded_by="XM_034319853.2:48..644"
                     /db_xref="GeneID:117602176"
ORIGIN      
        1 makvqlanva vldnpspfln pfqfevtfec ieelkedlew kmiyvgsaes eefdqvldti
       61 yvgpipegrh mfvfqadppd vsripvndal gvtvvlltcs yrghefvrvg yfinneytda
      121 elrenpppqp qfdkvqrnil gdkprvtrfk inwddsasst tnnvpegmdp qsleetsqda
      181 ptstlgftes thnsmevm
//
LOCUS       XP_034175759              89 aa            linear   INV 03-SEP-2025
DEFINITION  selenoprotein K isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175759
VERSION     XP_034175759.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319868.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175759.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="selenoprotein K isoform X1"
                     /calculated_mol_wt=9965
     Region          2..83
                     /region_name="SelK_SelG"
                     /note="Selenoprotein SelK_SelG; pfam10961"
                     /db_xref="CDD:463198"
     CDS             1..89
                     /gene="LOC117602185"
                     /coded_by="XM_034319868.2:147..416"
                     /db_xref="GeneID:117602185"
ORIGIN      
        1 mvyisddgkv ldttplhlkv frfftgiiym vvmffrtlin pdmnkhgsey trdyrpgsgp
       61 prpptrrlgr pntgntidap fggcrscar
//
LOCUS       XP_076546167              89 aa            linear   INV 03-SEP-2025
DEFINITION  selenoprotein K isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546167
VERSION     XP_076546167.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690052.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="selenoprotein K isoform X2"
                     /calculated_mol_wt=10045
     Region          3..83
                     /region_name="SelK_SelG"
                     /note="Selenoprotein SelK_SelG; pfam10961"
                     /db_xref="CDD:463198"
     CDS             1..89
                     /gene="LOC117602185"
                     /coded_by="XM_076690052.1:1..270"
                     /db_xref="GeneID:117602185"
ORIGIN      
        1 myfifsdgkv ldttplhlkv frfftgiiym vvmffrtlin pdmnkhgsey trdyrpgsgp
       61 prpptrrlgr pntgntidap fggcrscar
//
LOCUS       XP_034173185            5160 aa            linear   INV 03-SEP-2025
DEFINITION  hydrocephalus-inducing protein homolog isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173185
VERSION     XP_034173185.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317294.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173185.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..5160
                     /product="hydrocephalus-inducing protein homolog isoform
                     X1"
                     /calculated_mol_wt=588665
     Region          501..593
                     /region_name="ASH"
                     /note="Abnormal spindle-like microcephaly-assoc'd,
                     ASPM-SPD-2-Hydin; pfam15780"
                     /db_xref="CDD:464865"
     Region          2040..>2103
                     /region_name="Hydin_ADK"
                     /note="Hydin Adenylate kinase-like domain; pfam17213"
                     /db_xref="CDD:465383"
     CDS             1..5160
                     /gene="LOC117601046"
                     /coded_by="XM_034317294.2:503..15985"
                     /db_xref="GeneID:117601046"
ORIGIN      
        1 mneeindkil kesklfsate iiqyllersd ckknedekgq gaipseymrq msmtteertr
       61 hlfklrnkfn yfffcpedcn feitpsvvif qqftvgnalt vtvtvqnktk iprylktsce
      121 ldpcfsvehr gstysmmiap glancynvkf tpekkrdyqy llkfttdsgq mivpviaigs
      181 rgildfpdri elpstavkip stktifvrnv gnaeaiftly tndsrfwiep skgrieeees
      241 lqftvyfssn kagdfqgklf leyetgeklq idlqgsaenc tiridrgsvr meetylglsr
      301 sktltihnrs dyivkykwml lkdededkkr keeykklyhm vydaelircv dleyynvctp
      361 dihkliyqri yadelesltk esfpynhlff ifapqegeiw prsstgvtvf yraieigevt
      421 siaylevtgl enriplslyg igkgpvlrln vltidvaniy lcsvhnyeii vankghipgt
      481 lvhkpkpsdf ggtieitpic ltlepnqhks fnlsfssnrk gdfvervdfv vkeslevlsl
      541 hikgcivcpt lhfdkdslef attalgfstr hevnlnnlsl mpvaftitvl ddgdqpplth
      601 eeftkaptky sfpsnprefh iipeegvvsa qdvlkikity ianiaraght iiqvdmkdsd
      661 ldplqlpvkf ygrvaslliv psdivfrfcf infpysrsft vkntsdldgy fyilpqqvte
      721 htavicslsv yqgyikanqs ktinvtiitk vlgtekvtln mltmgeksss ticivtctgq
      781 gpvisvepiv ldfgdiqvle ektmellvin dspipahfng alekmnslwt mdpesgdlep
      841 dktititvkl flrdvgkfrn tvllsvlnsr tihveikvtg igcsvvfdps ifpvydlgll
      901 fshhlinrti tltnygtrah qiiwanepei qfyrgqilqt ksskfqlhpm ildipaeesr
      961 vvqcnlsweq necvledwyv fatiqgkgkr eligksifka tltkpqilfs kreltfrvdv
     1021 cpegdksqqk dellvtnqsn ldlnvqlsvk tpfyllttak ervqsmkvil idkattnisv
     1081 fffideqfkd lysrvdtgql lleymehpnq dkipckayvn fpnliiepkd ividcelgcs
     1141 aekvlaltnn gpvtvvykfl wiadsieidr dtnsdqvcpe csppqtgldf ykmenrksdd
     1201 vcvndrqdgs gdgplqsiqp ptnspvsetl klkpevssan dsetndctvt aeemreflmp
     1261 ifgkyflede divvlknlsf eppkdhyine vvkivpregt vppysvhhvy lgfhgfqrlr
     1321 vqakavceil rgpteeirli aradvtrfsi dtdvinfgqq mflgstrksf alrnlgtttf
     1381 qykingtilp ssnekidrld mnslkvepyt gyvdpqlfve iqvehcaeil gpihhrfqle
     1441 inhlmpvtvk viaygafpqv ypcipkgklh qyhsveleyf aiqsltedff inmlddinfk
     1501 egndlpkedi etltnegwci isyeetfprv mdidmaverh larkfvdans yiliqyasrq
     1561 knqpipqlfs teyiidmnyv ivdcvahysa kiinygpwdv emrmketrrk evlaksgilv
     1621 qfkkhsklvv ddsavlhitw hptrerfsvr stpvrhtihi qvihgctipi viqgivtypy
     1681 itvntklldf qevvvgeclv lsiliknegl idckweakls gtrkkqqety pfyvqynsnh
     1741 ippgheeiir infkpqqtcn veaklkiivk fslesqvitl igrgiekhln viepdiqfep
     1801 ivpftkiqet iftienpcdy pveffwhhld dsfqtedqii ktllyyyqak eiflpprkpg
     1861 dsiptsffqf yeqlvnemar tmvteepegt lledeqsdvn erketdaeqe rtskfnlskr
     1921 rlrkrdsrst krsrkktsvr gssrhgkrsd ifseassidl erndcptfsi lnesvvefvp
     1981 lptsdpeeiq rllfcyidal yqtpdilnkl kdpvkelfnd seeksddlpd pskpmkrvci
     2041 ifhgapftgy qetacksara lqipvlnidn aiteiialgg nscsiqlrqi iddayenyse
     2101 dferhknrln mkvdrkegte sptdaetqrd emtnepssrt kssrtkssrn psskksspkk
     2161 gkssrsnktd tsnaqqevfl klhaesdylk elnkvpaidv lelldplsry eykiqgllll
     2221 ekildggspr kkqnasflgi aqellveave erlsmedfes gfvlqslesn flcnsvvdvl
     2281 llllriighv eyflfvtffn smanynskvg elrreqaekm vdvgkkiqdi remtssqydl
     2341 lseqdkklyl dailpvkrae aakrkaryle rmmehkkkke vrgrvtnskt skvkenpksk
     2401 sgkinskkst nskhsskrdt tgskatrsrd sprqkkgvpt evkeitmamd qyyldllaik
     2461 nvisnwdpik killqespss aksripksak skdvgildta rekqapinnf hvwyvrssdp
     2521 wqdkiydmvt gqmsqnslak salpievvpe pdlepklysi lktrnfekrs stidestyql
     2581 vsissvpeip svvesiypse lsmqenraen rrnrksrgqr rnkaeeqrss ktedlslias
     2641 tidsidtnnv snletileep lkarwillpk echrfkirfq pevtgfheqm yaftivdgnn
     2701 vtyqvnvngi advpkldmnp ktifaktans klnetyipty fldsgiydfg tmlvlrkdkr
     2761 phrreaelkf cniskvdvel hfflaknnpe cffvqseell iaagnseslv lstiatkfgi
     2821 iseklylcvk nnpkveviel qhegtkldie lekkqisfdr vllyrmeyqs livrnksavp
     2881 lfwslkaeep aeaqitfsps egtieqqgeq kvefcyhgtk vgvtekkati feaflyeedk
     2941 eaiftdtill tgetydvqvd inyanpidln yikvgfpttg sftiknrgny evkyvitlee
     3001 kekleqlnlp knlkkdlqvq pvsgsipskk erivevvfvp kaelslknap ilkchlldtq
     3061 kgtaiiaeip ltvsliayyt rfrvhpypli dfgtlaicte ktmylnient gkyqlcytif
     3121 nplkhpsvly msqtakqvaa dsaekwskrk kksprndksh akdslevehl tmgpmtvtkm
     3181 mgqiepgetd tiaincfpef agsqeermtv lvndtvpenr ngktitlsvk savpridfhd
     3241 fdsmfqenhv vdriqdfecp keigphtvfa rqeaalyfrr irvlsthstc frlynrgvvs
     3301 anvdvhflsd slipetakqd tfqveprner iapmsqktft isfkptaint yqgnlqmiva
     3361 lpihlgeekl fiklvgescv pevaivepsh gkremaalnf prtlineasg ryfslentgl
     3421 ikakviveie edlndvfkls pvsdsrhflq lldkdhdesn drcvmillsp envarfkvaf
     3481 spdeigtfyg kvrmhiidnp yenlvinleg esylelvvld glefddnkrk kksrtssrlr
     3541 rlsskqnsli sipansslpv tltynldygl cfvskmykkt frifnksldr wfrfqwgahp
     3601 hvvfmptigh lkyqtckevv atflasepin hsnvpiecsl ceiilvdskg eapwddrqte
     3661 vkwekvrpei veqmnemell akkivqptae plheitpgts nsinlllnat vafseyscdv
     3721 eeihfkdtlm fqtreykftl tnpgivntly awkinmdeqy pkreqgdslh tfsrrkivte
     3781 sssqspsnak rifsittdfp hgkfdkyrqg gtgsvklfsl pctplqydar avstkssakk
     3841 sdlfsstagl sgrtadswve ddlpfsispe tgmlspqesv qftlkfspmd vfhykaylic
     3901 ridnidrnkm nltipvvgrs llpychfdiq esdyvtsgrr dgklpgpvgy diedsslwqn
     3961 irviefkvvg tggthvkkfh linpttddyh fswkdrtcrg vedisnfhct vmegmaergk
     4021 qtdlaftfla envgvfesfw lfsiarynlk clflvvgivt epsvhcltvh vklkptilgh
     4081 nvrdsirlln nedydipfai fenslysegk fqrlsvtpmt gvlvakqeqf lwveyhptrv
     4141 gefyfsiqcg iklmkrpltv fvtavvyeiv ssvsyispgh eiaqafqdkd nvidlgrlim
     4201 essttiefev knsgnvafyy twdlgitpei asrdaytilt sqkqghvfse sqstccltlk
     4261 tlrktiikdh cvllkitngp syrfllrass kkpaldfsfs rydfgpcyiq enngisyyke
     4321 llvqnsenva fiveckfeek phlsvdlasi sqalaansti sipitfkplk etkyrecllf
     4381 tinsanerki tilgegityk irlvnprdkc idlgsvsvsr tvtrripvvn eglasidvrf
     4441 dllknlssye qhrersqclt tqnvestpid lerasimetk rswtqdialq tkeprlsevl
     4501 kiepstnvvl kpnkrtnvlv tfkatsritp flskvalqtg stimplfvvq gscvaaefql
     4561 nrtrvsfgtv vqgytsetrv vlmntgdvga rfkwntsklp dvfkidplng ycspgmditf
     4621 mvsfqpyeea ttidgeatie iekygslrtk itggccqlpe ptetilfesf vreksvqsfv
     4681 imndthlswt lkpeitgdyf iveevlkipa kgfgscvity apltmnsedn lhtgtlllkl
     4741 pdentpilys lhgrsfppea taiitrqfpa ktkytesltv qnwlnrqqrf ickiellnnd
     4801 eeqskiplfk flgnnridvp angrrdyrav fysyeesefn fkvsfinedn eyqfyninye
     4861 vtkpeviesi kltasarskv chelkvenpl enktilytan cdhpfiaire lpktvppmsh
     4921 eyiyvlycpm ipseetitml nvnspelgqf pyelrlkalp pppekvtrvn atlgstcifp
     4981 llvhnytkep aefvinvddd cfvsqksisv lqldnrtiev ifepsnlent tatlrasskt
     5041 agdyifplig icslpkpmgp yiitrdipas ilfknvfree ktfefhvdps eifeieipst
     5101 mlhskqniei kvhlrkeeev vdketvmeek ypvtgklmvy cidhtlshvn wiyyvkgife
//
LOCUS       XP_034173194            5159 aa            linear   INV 03-SEP-2025
DEFINITION  hydrocephalus-inducing protein homolog isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173194
VERSION     XP_034173194.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317303.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173194.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..5159
                     /product="hydrocephalus-inducing protein homolog isoform
                     X2"
                     /calculated_mol_wt=588537
     Region          501..593
                     /region_name="ASH"
                     /note="Abnormal spindle-like microcephaly-assoc'd,
                     ASPM-SPD-2-Hydin; pfam15780"
                     /db_xref="CDD:464865"
     Region          2040..>2103
                     /region_name="Hydin_ADK"
                     /note="Hydin Adenylate kinase-like domain; pfam17213"
                     /db_xref="CDD:465383"
     CDS             1..5159
                     /gene="LOC117601046"
                     /coded_by="XM_034317303.2:503..15982"
                     /db_xref="GeneID:117601046"
ORIGIN      
        1 mneeindkil kesklfsate iiqyllersd ckknedekgq gaipseymrq msmtteertr
       61 hlfklrnkfn yfffcpedcn feitpsvvif qqftvgnalt vtvtvqnktk iprylktsce
      121 ldpcfsvehr gstysmmiap glancynvkf tpekkrdyqy llkfttdsgq mivpviaigs
      181 rgildfpdri elpstavkip stktifvrnv gnaeaiftly tndsrfwiep skgrieeees
      241 lqftvyfssn kagdfqgklf leyetgeklq idlqgsaenc tiridrgsvr meetylglsr
      301 sktltihnrs dyivkykwml lkdededkkr keeykklyhm vydaelircv dleyynvctp
      361 dihkliyqri yadelesltk esfpynhlff ifapqegeiw prsstgvtvf yraieigevt
      421 siaylevtgl enriplslyg igkgpvlrln vltidvaniy lcsvhnyeii vankghipgt
      481 lvhkpkpsdf ggtieitpic ltlepnqhks fnlsfssnrk gdfvervdfv vkeslevlsl
      541 hikgcivcpt lhfdkdslef attalgfstr hevnlnnlsl mpvaftitvl ddgdqpplth
      601 eeftkaptky sfpsnprefh iipeegvvsa qdvlkikity ianiaraght iiqvdmkdsd
      661 ldplqlpvkf ygrvaslliv psdivfrfcf infpysrsft vkntsdldgy fyilpqqvte
      721 htavicslsv yqgyikanqs ktinvtiitk vlgtekvtln mltmgeksss ticivtctgq
      781 gpvisvepiv ldfgdiqvle ektmellvin dspipahfng alekmnslwt mdpesgdlep
      841 dktititvkl flrdvgkfrn tvllsvlnsr tihveikvtg igcsvvfdps ifpvydlgll
      901 fshhlinrti tltnygtrah qiiwanepei qfyrgqilqt ksskfqlhpm ildipaeesr
      961 vvqcnlsweq necvledwyv fatiqgkgkr eligksifka tltkpqilfs kreltfrvdv
     1021 cpegdksqqk dellvtnqsn ldlnvqlsvk tpfyllttak ervqsmkvil idkattnisv
     1081 fffideqfkd lysrvdtgql lleymehpnq dkipckayvn fpnliiepkd ividcelgcs
     1141 aekvlaltnn gpvtvvykfl wiadsieidr dtnsdqvcpe csppqtgldf ykmenrksdd
     1201 vcvndrqdgs gdgplqsiqp ptnspvsetl klkpevssan dsetndctvt aeemreflmp
     1261 ifgkyflede divvlknlsf eppkdhyine vvkivpregt vppysvhhvy lgfhgfqrlr
     1321 vqakavceil rgpteeirli aradvtrfsi dtdvinfgqq mflgstrksf alrnlgtttf
     1381 qykingtilp ssnekidrld mnslkvepyt gyvdpqlfve iqvehcaeil gpihhrfqle
     1441 inhlmpvtvk viaygafpqv ypcipkgklh qyhsveleyf aiqsltedff inmlddinfk
     1501 egndlpkedi etltnegwci isyeetfprv mdidmaverh larkfvdans yiliqyasrq
     1561 knqpipqlfs teyiidmnyv ivdcvahysa kiinygpwdv emrmketrrk evlaksgilv
     1621 qfkkhsklvv ddsavlhitw hptrerfsvr stpvrhtihi qvihgctipi viqgivtypy
     1681 itvntklldf qevvvgeclv lsiliknegl idckweakls gtrkkqqety pfyvqynsnh
     1741 ippgheeiir infkpqqtcn veaklkiivk fslesqvitl igrgiekhln viepdiqfep
     1801 ivpftkiqet iftienpcdy pveffwhhld dsfqtedqii ktllyyyqak eiflpprkpg
     1861 dsiptsffqf yeqlvnemar tmvteepegt lledeqsdvn erketdaeqe rtskfnlskr
     1921 rlrkrdsrst krsrkktsvr gssrhgkrsd ifseassidl erndcptfsi lnesvvefvp
     1981 lptsdpeeiq rllfcyidal yqtpdilnkl kdpvkelfnd seeksddlpd pskpmkrvci
     2041 ifhgapftgy qetacksara lqipvlnidn aiteiialgg nscsiqlrqi iddayenyse
     2101 dferhknrln mkvdrkegte sptdaetqrd emtnepssrt kssrtkssrn psskksspkk
     2161 gkssrsnktd tsnaqqevfl klhaesdylk elnkvpaidv lelldplsry eykiqgllll
     2221 ekildggspr kkqnasflgi aqellveave erlsmedfes gfvlqslesn flcnsvvdvl
     2281 llllriighv eyflfvtffn smanynskvg elrreqaekm vdvgkkiqdi remtssqydl
     2341 lseqdkklyl dailpvkrae aakrkaryle rmmehkkkke vrgrvtnskt skvkenpksk
     2401 sgkinskkst nskhsskrdt tgskatrsrd sprqkkgvpt evkeitmamd qyyldllaik
     2461 nvisnwdpik killqespss aksripksak skdvgildta reqapinnfh vwyvrssdpw
     2521 qdkiydmvtg qmsqnslaks alpievvpep dlepklysil ktrnfekrss tidestyqlv
     2581 sissvpeips vvesiypsel smqenraenr rnrksrgqrr nkaeeqrssk tedlsliast
     2641 idsidtnnvs nletileepl karwillpke chrfkirfqp evtgfheqmy aftivdgnnv
     2701 tyqvnvngia dvpkldmnpk tifaktansk lnetyiptyf ldsgiydfgt mlvlrkdkrp
     2761 hrreaelkfc niskvdvelh fflaknnpec ffvqseelli aagnseslvl stiatkfgii
     2821 seklylcvkn npkvevielq hegtkldiel ekkqisfdrv llyrmeyqsl ivrnksavpl
     2881 fwslkaeepa eaqitfspse gtieqqgeqk vefcyhgtkv gvtekkatif eaflyeedke
     2941 aiftdtillt getydvqvdi nyanpidlny ikvgfpttgs ftiknrgnye vkyvitleek
     3001 ekleqlnlpk nlkkdlqvqp vsgsipskke rivevvfvpk aelslknapi lkchlldtqk
     3061 gtaiiaeipl tvsliayytr frvhpyplid fgtlaictek tmylnientg kyqlcytifn
     3121 plkhpsvlym sqtakqvaad saekwskrkk ksprndksha kdslevehlt mgpmtvtkmm
     3181 gqiepgetdt iaincfpefa gsqeermtvl vndtvpenrn gktitlsvks avpridfhdf
     3241 dsmfqenhvv driqdfecpk eigphtvfar qeaalyfrri rvlsthstcf rlynrgvvsa
     3301 nvdvhflsds lipetakqdt fqveprneri apmsqktfti sfkptainty qgnlqmival
     3361 pihlgeeklf iklvgescvp evaivepshg kremaalnfp rtlineasgr yfslentgli
     3421 kakviveiee dlndvfklsp vsdsrhflql ldkdhdesnd rcvmillspe nvarfkvafs
     3481 pdeigtfygk vrmhiidnpy enlvinlege sylelvvldg lefddnkrkk ksrtssrlrr
     3541 lsskqnslis ipansslpvt ltynldyglc fvskmykktf rifnksldrw frfqwgahph
     3601 vvfmptighl kyqtckevva tflasepinh snvpiecslc eiilvdskge apwddrqtev
     3661 kwekvrpeiv eqmnemella kkivqptaep lheitpgtsn sinlllnatv afseyscdve
     3721 eihfkdtlmf qtreykftlt npgivntlya wkinmdeqyp kreqgdslht fsrrkivtes
     3781 ssqspsnakr ifsittdfph gkfdkyrqgg tgsvklfslp ctplqydara vstkssakks
     3841 dlfsstagls grtadswved dlpfsispet gmlspqesvq ftlkfspmdv fhykaylicr
     3901 idnidrnkmn ltipvvgrsl lpychfdiqe sdyvtsgrrd gklpgpvgyd iedsslwqni
     3961 rviefkvvgt ggthvkkfhl inpttddyhf swkdrtcrgv edisnfhctv megmaergkq
     4021 tdlaftflae nvgvfesfwl fsiarynlkc lflvvgivte psvhcltvhv klkptilghn
     4081 vrdsirllnn edydipfaif enslysegkf qrlsvtpmtg vlvakqeqfl wveyhptrvg
     4141 efyfsiqcgi klmkrpltvf vtavvyeivs svsyispghe iaqafqdkdn vidlgrlime
     4201 ssttiefevk nsgnvafyyt wdlgitpeia srdaytilts qkqghvfses qstccltlkt
     4261 lrktiikdhc vllkitngps yrfllrassk kpaldfsfsr ydfgpcyiqe nngisyykel
     4321 lvqnsenvaf iveckfeekp hlsvdlasis qalaanstis ipitfkplke tkyrecllft
     4381 insanerkit ilgegityki rlvnprdkci dlgsvsvsrt vtrripvvne glasidvrfd
     4441 llknlssyeq hrersqcltt qnvestpidl erasimetkr swtqdialqt keprlsevlk
     4501 iepstnvvlk pnkrtnvlvt fkatsritpf lskvalqtgs timplfvvqg scvaaefqln
     4561 rtrvsfgtvv qgytsetrvv lmntgdvgar fkwntsklpd vfkidplngy cspgmditfm
     4621 vsfqpyeeat tidgeatiei ekygslrtki tggccqlpep tetilfesfv reksvqsfvi
     4681 mndthlswtl kpeitgdyfi veevlkipak gfgscvitya pltmnsednl htgtlllklp
     4741 dentpilysl hgrsfppeat aiitrqfpak tkytesltvq nwlnrqqrfi ckiellnnde
     4801 eqskiplfkf lgnnridvpa ngrrdyravf ysyeesefnf kvsfinedne yqfyninyev
     4861 tkpeviesik ltasarskvc helkvenple nktilytanc dhpfiairel pktvppmshe
     4921 yiyvlycpmi pseetitmln vnspelgqfp yelrlkalpp ppekvtrvna tlgstcifpl
     4981 lvhnytkepa efvinvdddc fvsqksisvl qldnrtievi fepsnlentt atlrasskta
     5041 gdyifpligi cslpkpmgpy iitrdipasi lfknvfreek tfefhvdpse ifeieipstm
     5101 lhskqnieik vhlrkeeevv dketvmeeky pvtgklmvyc idhtlshvnw iyyvkgife
//
LOCUS       XP_034173204            5159 aa            linear   INV 03-SEP-2025
DEFINITION  hydrocephalus-inducing protein homolog isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173204
VERSION     XP_034173204.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317313.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173204.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..5159
                     /product="hydrocephalus-inducing protein homolog isoform
                     X3"
                     /calculated_mol_wt=588537
     Region          500..592
                     /region_name="ASH"
                     /note="Abnormal spindle-like microcephaly-assoc'd,
                     ASPM-SPD-2-Hydin; pfam15780"
                     /db_xref="CDD:464865"
     Region          2039..>2102
                     /region_name="Hydin_ADK"
                     /note="Hydin Adenylate kinase-like domain; pfam17213"
                     /db_xref="CDD:465383"
     CDS             1..5159
                     /gene="LOC117601046"
                     /coded_by="XM_034317313.2:503..15982"
                     /db_xref="GeneID:117601046"
ORIGIN      
        1 mneeindkil kesklfsate iiqyllersd ckknedekgg aipseymrqm smtteertrh
       61 lfklrnkfny fffcpedcnf eitpsvvifq qftvgnaltv tvtvqnktki prylktscel
      121 dpcfsvehrg stysmmiapg lancynvkft pekkrdyqyl lkfttdsgqm ivpviaigsr
      181 gildfpdrie lpstavkips tktifvrnvg naeaiftlyt ndsrfwieps kgrieeeesl
      241 qftvyfssnk agdfqgklfl eyetgeklqi dlqgsaenct iridrgsvrm eetylglsrs
      301 ktltihnrsd yivkykwmll kdededkkrk eeykklyhmv ydaelircvd leyynvctpd
      361 ihkliyqriy adelesltke sfpynhlffi fapqegeiwp rsstgvtvfy raieigevts
      421 iaylevtgle nriplslygi gkgpvlrlnv ltidvaniyl csvhnyeiiv ankghipgtl
      481 vhkpkpsdfg gtieitpicl tlepnqhksf nlsfssnrkg dfvervdfvv keslevlslh
      541 ikgcivcptl hfdkdslefa ttalgfstrh evnlnnlslm pvaftitvld dgdqpplthe
      601 eftkaptkys fpsnprefhi ipeegvvsaq dvlkikityi aniaraghti iqvdmkdsdl
      661 dplqlpvkfy grvasllivp sdivfrfcfi nfpysrsftv kntsdldgyf yilpqqvteh
      721 tavicslsvy qgyikanqsk tinvtiitkv lgtekvtlnm ltmgekssst icivtctgqg
      781 pvisvepivl dfgdiqvlee ktmellvind spipahfnga lekmnslwtm dpesgdlepd
      841 ktititvklf lrdvgkfrnt vllsvlnsrt ihveikvtgi gcsvvfdpsi fpvydlgllf
      901 shhlinrtit ltnygtrahq iiwanepeiq fyrgqilqtk sskfqlhpmi ldipaeesrv
      961 vqcnlsweqn ecvledwyvf atiqgkgkre ligksifkat ltkpqilfsk reltfrvdvc
     1021 pegdksqqkd ellvtnqsnl dlnvqlsvkt pfyllttake rvqsmkvili dkattnisvf
     1081 ffideqfkdl ysrvdtgqll leymehpnqd kipckayvnf pnliiepkdi vidcelgcsa
     1141 ekvlaltnng pvtvvykflw iadsieidrd tnsdqvcpec sppqtgldfy kmenrksddv
     1201 cvndrqdgsg dgplqsiqpp tnspvsetlk lkpevssand setndctvta eemreflmpi
     1261 fgkyfleded ivvlknlsfe ppkdhyinev vkivpregtv ppysvhhvyl gfhgfqrlrv
     1321 qakavceilr gpteeirlia radvtrfsid tdvinfgqqm flgstrksfa lrnlgtttfq
     1381 ykingtilps snekidrldm nslkvepytg yvdpqlfvei qvehcaeilg pihhrfqlei
     1441 nhlmpvtvkv iaygafpqvy pcipkgklhq yhsveleyfa iqsltedffi nmlddinfke
     1501 gndlpkedie tltnegwcii syeetfprvm didmaverhl arkfvdansy iliqyasrqk
     1561 nqpipqlfst eyiidmnyvi vdcvahysak iinygpwdve mrmketrrke vlaksgilvq
     1621 fkkhsklvvd dsavlhitwh ptrerfsvrs tpvrhtihiq vihgctipiv iqgivtypyi
     1681 tvntklldfq evvvgeclvl siliknegli dckweaklsg trkkqqetyp fyvqynsnhi
     1741 ppgheeiiri nfkpqqtcnv eaklkiivkf slesqvitli grgiekhlnv iepdiqfepi
     1801 vpftkiqeti ftienpcdyp veffwhhldd sfqtedqiik tllyyyqake iflpprkpgd
     1861 siptsffqfy eqlvnemart mvteepegtl ledeqsdvne rketdaeqer tskfnlskrr
     1921 lrkrdsrstk rsrkktsvrg ssrhgkrsdi fseassidle rndcptfsil nesvvefvpl
     1981 ptsdpeeiqr llfcyidaly qtpdilnklk dpvkelfnds eeksddlpdp skpmkrvcii
     2041 fhgapftgyq etacksaral qipvlnidna iteiialggn scsiqlrqii ddayenysed
     2101 ferhknrlnm kvdrkegtes ptdaetqrde mtnepssrtk ssrtkssrnp sskksspkkg
     2161 kssrsnktdt snaqqevflk lhaesdylke lnkvpaidvl elldplsrye ykiqglllle
     2221 kildggsprk kqnasflgia qellveavee rlsmedfesg fvlqslesnf lcnsvvdvll
     2281 lllriighve yflfvtffns manynskvge lrreqaekmv dvgkkiqdir emtssqydll
     2341 seqdkklyld ailpvkraea akrkaryler mmehkkkkev rgrvtnskts kvkenpksks
     2401 gkinskkstn skhsskrdtt gskatrsrds prqkkgvpte vkeitmamdq yyldllaikn
     2461 visnwdpikk illqespssa ksripksaks kdvgildtar ekqapinnfh vwyvrssdpw
     2521 qdkiydmvtg qmsqnslaks alpievvpep dlepklysil ktrnfekrss tidestyqlv
     2581 sissvpeips vvesiypsel smqenraenr rnrksrgqrr nkaeeqrssk tedlsliast
     2641 idsidtnnvs nletileepl karwillpke chrfkirfqp evtgfheqmy aftivdgnnv
     2701 tyqvnvngia dvpkldmnpk tifaktansk lnetyiptyf ldsgiydfgt mlvlrkdkrp
     2761 hrreaelkfc niskvdvelh fflaknnpec ffvqseelli aagnseslvl stiatkfgii
     2821 seklylcvkn npkvevielq hegtkldiel ekkqisfdrv llyrmeyqsl ivrnksavpl
     2881 fwslkaeepa eaqitfspse gtieqqgeqk vefcyhgtkv gvtekkatif eaflyeedke
     2941 aiftdtillt getydvqvdi nyanpidlny ikvgfpttgs ftiknrgnye vkyvitleek
     3001 ekleqlnlpk nlkkdlqvqp vsgsipskke rivevvfvpk aelslknapi lkchlldtqk
     3061 gtaiiaeipl tvsliayytr frvhpyplid fgtlaictek tmylnientg kyqlcytifn
     3121 plkhpsvlym sqtakqvaad saekwskrkk ksprndksha kdslevehlt mgpmtvtkmm
     3181 gqiepgetdt iaincfpefa gsqeermtvl vndtvpenrn gktitlsvks avpridfhdf
     3241 dsmfqenhvv driqdfecpk eigphtvfar qeaalyfrri rvlsthstcf rlynrgvvsa
     3301 nvdvhflsds lipetakqdt fqveprneri apmsqktfti sfkptainty qgnlqmival
     3361 pihlgeeklf iklvgescvp evaivepshg kremaalnfp rtlineasgr yfslentgli
     3421 kakviveiee dlndvfklsp vsdsrhflql ldkdhdesnd rcvmillspe nvarfkvafs
     3481 pdeigtfygk vrmhiidnpy enlvinlege sylelvvldg lefddnkrkk ksrtssrlrr
     3541 lsskqnslis ipansslpvt ltynldyglc fvskmykktf rifnksldrw frfqwgahph
     3601 vvfmptighl kyqtckevva tflasepinh snvpiecslc eiilvdskge apwddrqtev
     3661 kwekvrpeiv eqmnemella kkivqptaep lheitpgtsn sinlllnatv afseyscdve
     3721 eihfkdtlmf qtreykftlt npgivntlya wkinmdeqyp kreqgdslht fsrrkivtes
     3781 ssqspsnakr ifsittdfph gkfdkyrqgg tgsvklfslp ctplqydara vstkssakks
     3841 dlfsstagls grtadswved dlpfsispet gmlspqesvq ftlkfspmdv fhykaylicr
     3901 idnidrnkmn ltipvvgrsl lpychfdiqe sdyvtsgrrd gklpgpvgyd iedsslwqni
     3961 rviefkvvgt ggthvkkfhl inpttddyhf swkdrtcrgv edisnfhctv megmaergkq
     4021 tdlaftflae nvgvfesfwl fsiarynlkc lflvvgivte psvhcltvhv klkptilghn
     4081 vrdsirllnn edydipfaif enslysegkf qrlsvtpmtg vlvakqeqfl wveyhptrvg
     4141 efyfsiqcgi klmkrpltvf vtavvyeivs svsyispghe iaqafqdkdn vidlgrlime
     4201 ssttiefevk nsgnvafyyt wdlgitpeia srdaytilts qkqghvfses qstccltlkt
     4261 lrktiikdhc vllkitngps yrfllrassk kpaldfsfsr ydfgpcyiqe nngisyykel
     4321 lvqnsenvaf iveckfeekp hlsvdlasis qalaanstis ipitfkplke tkyrecllft
     4381 insanerkit ilgegityki rlvnprdkci dlgsvsvsrt vtrripvvne glasidvrfd
     4441 llknlssyeq hrersqcltt qnvestpidl erasimetkr swtqdialqt keprlsevlk
     4501 iepstnvvlk pnkrtnvlvt fkatsritpf lskvalqtgs timplfvvqg scvaaefqln
     4561 rtrvsfgtvv qgytsetrvv lmntgdvgar fkwntsklpd vfkidplngy cspgmditfm
     4621 vsfqpyeeat tidgeatiei ekygslrtki tggccqlpep tetilfesfv reksvqsfvi
     4681 mndthlswtl kpeitgdyfi veevlkipak gfgscvitya pltmnsednl htgtlllklp
     4741 dentpilysl hgrsfppeat aiitrqfpak tkytesltvq nwlnrqqrfi ckiellnnde
     4801 eqskiplfkf lgnnridvpa ngrrdyravf ysyeesefnf kvsfinedne yqfyninyev
     4861 tkpeviesik ltasarskvc helkvenple nktilytanc dhpfiairel pktvppmshe
     4921 yiyvlycpmi pseetitmln vnspelgqfp yelrlkalpp ppekvtrvna tlgstcifpl
     4981 lvhnytkepa efvinvdddc fvsqksisvl qldnrtievi fepsnlentt atlrasskta
     5041 gdyifpligi cslpkpmgpy iitrdipasi lfknvfreek tfefhvdpse ifeieipstm
     5101 lhskqnieik vhlrkeeevv dketvmeeky pvtgklmvyc idhtlshvnw iyyvkgife
//
LOCUS       XP_034173331            2308 aa            linear   INV 03-SEP-2025
DEFINITION  ELKS/RAB6-interacting/CAST family member bruchpilot isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034173331
VERSION     XP_034173331.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317440.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173331.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2308
                     /product="ELKS/RAB6-interacting/CAST family member
                     bruchpilot isoform X1"
                     /calculated_mol_wt=265889
     Region          193..>344
                     /region_name="Cast"
                     /note="RIM-binding protein of the cytomatrix active zone;
                     pfam10174"
                     /db_xref="CDD:431111"
     Region          <781..1230
                     /region_name="Cast"
                     /note="RIM-binding protein of the cytomatrix active zone;
                     pfam10174"
                     /db_xref="CDD:431111"
     Region          934..1746
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          1375..2215
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..2308
                     /gene="brp"
                     /coded_by="XM_034317440.2:2111..9037"
                     /db_xref="GeneID:117601062"
ORIGIN      
        1 msrdpyssgs gvvgpgggtr sprsgrrvge lptvdrspsr syasgrgspl grkrgtrsgn
       61 nspehlgygs yhhhqlgspy ysrdedlasp vmleergrsm stgggggghh rsrsasrpam
      121 sssagmsary tsldrasagi ldhdrefvpi rdprersrdr glyledelyg srsarqspnp
      181 hmlrdsggyi gelqhqnndl qrelgnlkke leltnqklgs smhsiktfws pelkkeralr
      241 keestkysli neqlkllnse nqkqsmmvrq leeelrmrvr gpsvelqqqm evlynenehl
      301 treiailrdt ikelelriet qkqtlqarde sikkllemlq nkgmdrdrtk iwtpsrppks
      361 fsllsavpgk eeerimfqqm qsmaqkqtrs cpsrltaima nrrnrctssg tvrksssrvk
      421 asrsqaqgrt lrlfgtdlmr haiirrrspr elkatneslk slqtqlelay astassgaav
      481 aggclglgsv sdggggtvlg gsvavpggvp gsttltaime tkdariqtle kevalleael
      541 qrikecggrl reqllkaiav psgcttgigd iqqaqqqqqq qhqqqlqqas mieqaqqqqq
      601 qqnqvatggg giesqlrqgl vtamgggpqq pgtlalargp svmtslvpyg lssstaltss
      661 llagstggtm sggaggggss clagissgat tgvvnpytdr yadhhlhqly hhhlpphhhh
      721 hhhqpyhlhq qqqqqqqqqq qqlqlqqqlq lqqqhqqqmq qhlkhiepaa qgalalhaht
      781 fnkhdmnfkr qldelrtevq rrdqellams akmktleeqh qdyqrhitvl keslcakeeh
      841 ynmlqadvee mrqrleeknr miekktqaam qaqqernrmn teltelkdhm dikdrkinvl
      901 qrkienledl lkekdnqvdm ararltamqa hhcssegals sleeaigdke kqmaqlreqr
      961 draeqdkqee relhereiae ykmkihales eveklgarle raqaekdrle aklessqsel
     1021 gkskaeldka asevgrsgad weaakqrltr lelenerlrh dlersqttyg rsslnstqem
     1081 driqeraekt aaelrraqae lrvtqadner araeaaalqe kveksqgevy rlkarlenaq
     1141 geqeslrdey draqasvarl hserdkaige lektqeeler tqatlgkaql qqdklqnlld
     1201 ksqsevdklq ekldktqtei rrmqmerekq nydfenlqsq ldkalgqstr mqkeretiql
     1261 evdrlqdkye kaqmimqrlq kerdgfqeev eklheriefq qnqiskmqre kenvlseldl
     1321 vkerwekahn shqkltlerd dalteieilk eklekaqysm nkaheerena nkefekmlek
     1381 ydraqsevyr lqnrievmea dkdrleleae kqqmlaaksr dearkaqeel arvqemydra
     1441 alqlgrtkeh eekskedldr lsvdlemvre ryeksqiemr rlqnereklv adnerisfel
     1501 erahsqlnka qaatektqee larmqleiek myekhdrqqa evrkaqgeae rlrveaesar
     1561 eeceryaarf gkyqesqerq keehewakle verlrdrlek aqtelerark aeqdaqrlra
     1621 dleraegvrg kyqyeqekwq segsrlqqev eklrerlegr eselkrtqss naeleaklhe
     1681 tqlqlerare etskvtaaqe rnrgeierar ietekirdrh dkvkaradal eadneklrve
     1741 ivrlerlmqt egktrsesas ieverlrasl dkaivardqv eleagrlake lekahmqtak
     1801 yqeaaeankk elerlaaeiq llrderealr qelrrvqqqq qqqmagseel ahmqqelkva
     1861 qlerdkmaai lenrekhsek iekmkeklea eakqlqverd qlviqleksq emlvnfqqel
     1921 naneaelerq rdevrrlqql qqqrahaqtp draakealea qmrevhrlnq qmqtltqaqt
     1981 kerqraeqae krvqelqkqi atressaaaa gandaqleqw rklcetekqr adaaerqaae
     2041 lqkriqtter qvhaqqqqiq qmqsamqqqq qqlqqqlqqp qqsqqngqea arlrkelera
     2101 redikqaave rerfqaqlem lcqeleknqv dlheankklq agaakagtdt tqerrqleeq
     2161 rrqleeqrrq meeraksvdq kvrtieeker tlqsldqdlk krkakmdqle qqlqrsggsp
     2221 dkrlaemqka lettekelek akaestrsaa eterllqlmq mtqeeqntke rqirelqdal
     2281 kaaqaklkqa ataqqqeagp agflkslf
//
LOCUS       XP_076546168            1904 aa            linear   INV 03-SEP-2025
DEFINITION  ELKS/RAB6-interacting/CAST family member bruchpilot isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_076546168
VERSION     XP_076546168.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690053.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1904
                     /product="ELKS/RAB6-interacting/CAST family member
                     bruchpilot isoform X2"
                     /calculated_mol_wt=222625
     Region          193..785
                     /region_name="Cast"
                     /note="RIM-binding protein of the cytomatrix active zone;
                     pfam10174"
                     /db_xref="CDD:431111"
     Region          530..1342
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          971..1811
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1904
                     /gene="brp"
                     /coded_by="XM_076690053.1:2105..7819"
                     /db_xref="GeneID:117601062"
ORIGIN      
        1 msrdpyssgs gvvgpgggtr sprsgrrvge lptvdrspsr syasgrgspl grkrgtrsgn
       61 nspehlgygs yhhhqlgspy ysrdedlasp vmleergrsm stgggggghh rsrsasrpam
      121 sssagmsary tsldrasagi ldhdrefvpi rdprersrdr glyledelyg srsarqspnp
      181 hmlrdsggyi gelqhqnndl qrelgnlkke leltnqklgs smhsiktfws pelkkeralr
      241 keestkysli neqlkllnse nqkqsmmvrq leeelrmrvr gpsvelqqqm evlynenehl
      301 treiailrdt ikelelriet qkqtlqarde sikkllemlq nkgmdrdrtk iwtpsrppks
      361 fsllsavpgk eeerimfqqm qsmaqkqlde lrtevqrrdq ellamsakmk tleeqhqdyq
      421 rhitvlkesl cakeehynml qadveemrqr leeknrmiek ktqaamqaqq ernrmntelt
      481 elkdhmdikd rkinvlqrki enledllkek dnqvdmarar ltamqahhcs segalsslee
      541 aigdkekqma qlreqrdrae qdkqeerelh ereiaeykmk ihalesevek lgarleraqa
      601 ekdrleakle ssqselgksk aeldkaasev grsgadweaa kqrltrlele nerlrhdler
      661 sqttygrssl nstqemdriq eraektaael rraqaelrvt qadnerarae aaalqekvek
      721 sqgevyrlka rlenaqgeqe slrdeydraq asvarlhser dkaigelekt qeelertqat
      781 lgkaqlqqdk lqnlldksqs evdklqekld ktqteirrmq merekqnydf enlqsqldka
      841 lgqstrmqke retiqlevdr lqdkyekaqm imqrlqkerd gfqeeveklh eriefqqnqi
      901 skmqrekenv lseldlvker wekahnshqk ltlerddalt eieilkekle kaqysmnkah
      961 eerenankef ekmlekydra qsevyrlqnr ievmeadkdr leleaekqqm laaksrdear
     1021 kaqeelarvq emydraalql grtkeheeks kedldrlsvd lemvreryek sqiemrrlqn
     1081 ereklvadne risfelerah sqlnkaqaat ektqeelarm qleiekmyek hdrqqaevrk
     1141 aqgeaerlrv eaesareece ryaarfgkyq esqerqkeeh ewakleverl rdrlekaqte
     1201 lerarkaeqd aqrlradler aegvrgkyqy eqekwqsegs rlqqeveklr erlegresel
     1261 krtqssnael eaklhetqlq lerareetsk vtaaqernrg eierariete kirdrhdkvk
     1321 aradaleadn eklrveivrl erlmqtegkt rsesasieve rlrasldkai vardqvelea
     1381 grlakeleka hmqtakyqea aeankkeler laaeiqllrd erealrqelr rvqqqqqqqm
     1441 agseelahmq qelkvaqler dkmaailenr ekhsekiekm kekleaeakq lqverdqlvi
     1501 qleksqemlv nfqqelnane aelerqrdev rrlqqlqqqr ahaqtpdraa kealeaqmre
     1561 vhrlnqqmqt ltqaqtkerq raeqaekrvq elqkqiatre ssaaaagand aqleqwrklc
     1621 etekqradaa erqaaelqkr iqtterqvha qqqqiqqmqs amqqqqqqlq qqlqqpqqsq
     1681 qngqeaarlr keleraredi kqaavererf qaqlemlcqe leknqvdlhe ankklqagaa
     1741 kagtdttqer rqleeqrrql eeqrrqmeer aksvdqkvrt ieekertlqs ldqdlkkrka
     1801 kmdqleqqlq rsggspdkrl aemqkalett ekelekakae strsaaeter llqlmqmtqe
     1861 eqntkerqir elqdalkaaq aklkqaataq qqeagpagfl kslf
//
LOCUS       XP_076546169            1880 aa            linear   INV 03-SEP-2025
DEFINITION  ELKS/RAB6-interacting/CAST family member bruchpilot isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546169
VERSION     XP_076546169.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690054.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1880
                     /product="ELKS/RAB6-interacting/CAST family member
                     bruchpilot isoform X3"
                     /calculated_mol_wt=219888
     Region          193..761
                     /region_name="Cast"
                     /note="RIM-binding protein of the cytomatrix active zone;
                     pfam10174"
                     /db_xref="CDD:431111"
     Region          506..1318
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          947..1787
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1880
                     /gene="brp"
                     /coded_by="XM_076690054.1:2104..7746"
                     /db_xref="GeneID:117601062"
ORIGIN      
        1 msrdpyssgs gvvgpgggtr sprsgrrvge lptvdrspsr syasgrgspl grkrgtrsgn
       61 nspehlgygs yhhhqlgspy ysrdedlasp vmleergrsm stgggggghh rsrsasrpam
      121 sssagmsary tsldrasagi ldhdrefvpi rdprersrdr glyledelyg srsarqspnp
      181 hmlrdsggyi gelqhqnndl qrelgnlkke leltnqklgs smhsiktfws pelkkeralr
      241 keestkysli neqlkllnse nqkqsmmvrq leeelrmrvr gpsvelqqqm evlynenehl
      301 treiailrdt ikelelriet qkqtlqarde sikkllemlq nkgmgkeeer imfqqmqsma
      361 qkqldelrte vqrrdqella msakmktlee qhqdyqrhit vlkeslcake ehynmlqadv
      421 eemrqrleek nrmiekktqa amqaqqernr mnteltelkd hmdikdrkin vlqrkienle
      481 dllkekdnqv dmararltam qahhcssega lssleeaigd kekqmaqlre qrdraeqdkq
      541 eerelherei aeykmkihal eseveklgar leraqaekdr leaklessqs elgkskaeld
      601 kaasevgrsg adweaakqrl trlelenerl rhdlersqtt ygrsslnstq emdriqerae
      661 ktaaelrraq aelrvtqadn eraraeaaal qekveksqge vyrlkarlen aqgeqeslrd
      721 eydraqasva rlhserdkai gelektqeel ertqatlgka qlqqdklqnl ldksqsevdk
      781 lqekldktqt eirrmqmere kqnydfenlq sqldkalgqs trmqkereti qlevdrlqdk
      841 yekaqmimqr lqkerdgfqe eveklherie fqqnqiskmq rekenvlsel dlvkerweka
      901 hnshqkltle rddalteiei lkeklekaqy smnkaheere nankefekml ekydraqsev
      961 yrlqnrievm eadkdrlele aekqqmlaak srdearkaqe elarvqemyd raalqlgrtk
     1021 eheekskedl drlsvdlemv reryeksqie mrrlqnerek lvadnerisf elerahsqln
     1081 kaqaatektq eelarmqlei ekmyekhdrq qaevrkaqge aerlrveaes areeceryaa
     1141 rfgkyqesqe rqkeehewak leverlrdrl ekaqtelera rkaeqdaqrl radleraegv
     1201 rgkyqyeqek wqsegsrlqq eveklrerle greselkrtq ssnaeleakl hetqlqlera
     1261 reetskvtaa qernrgeier arietekird rhdkvkarad aleadneklr veivrlerlm
     1321 qtegktrses asieverlra sldkaivard qveleagrla kelekahmqt akyqeaaean
     1381 kkelerlaae iqllrderea lrqelrrvqq qqqqqmagse elahmqqelk vaqlerdkma
     1441 ailenrekhs ekiekmkekl eaeakqlqve rdqlviqlek sqemlvnfqq elnaneaele
     1501 rqrdevrrlq qlqqqrahaq tpdraakeal eaqmrevhrl nqqmqtltqa qtkerqraeq
     1561 aekrvqelqk qiatressaa aagandaqle qwrklcetek qradaaerqa aelqkriqtt
     1621 erqvhaqqqq iqqmqsamqq qqqqlqqqlq qpqqsqqngq eaarlrkele raredikqaa
     1681 vererfqaql emlcqelekn qvdlheankk lqagaakagt dttqerrqle eqrrqleeqr
     1741 rqmeeraksv dqkvrtieek ertlqsldqd lkkrkakmdq leqqlqrsgg spdkrlaemq
     1801 kalettekel ekakaestrs aaeterllql mqmtqeeqnt kerqirelqd alkaaqaklk
     1861 qaataqqqea gpagflkslf
//
LOCUS       XP_076546170             726 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305652 [Osmia lignaria lignaria].
ACCESSION   XP_076546170
VERSION     XP_076546170.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690055.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..726
                     /product="uncharacterized protein LOC143305652"
                     /calculated_mol_wt=85185
     Region          <72..353
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <214..660
                     /region_name="Cast"
                     /note="RIM-binding protein of the cytomatrix active zone;
                     pfam10174"
                     /db_xref="CDD:431111"
     CDS             1..726
                     /gene="LOC143305652"
                     /coded_by="XM_076690055.1:1..2181"
                     /db_xref="GeneID:143305652"
ORIGIN      
        1 mknrsthtgl epcvslsvsl ffflhffvfl fafttfahdd artqqaersa tspenargre
       61 darkmeqeak drricelelk asclekklls kecskrmkda rmsvednegw kkelemknrr
      121 iaeledelva cktvknsnne ytqslkllde lkeekdswrr emevknkrit qledeierlk
      181 illdengeas nfkddkskie dnnselwrme ikaknrrive leqemlslen flrehadtes
      241 vrdleirier karrieeled tvaefedflk qnpdvkelhd lrcqvtagnr riqelerwiq
      301 kngenresri dqervielek mvteleeyvk ehnvdvlkrk lqdrecridq lqdrvgclek
      361 ellkveenrr entskdrqdk etttkpehlp cqhsvakest sefcdkeekm kkmehaisek
      421 dnklqeyeqq ivenkeeitk lrkeaallkk elseyddigv lkeeirvrde riqqledeld
      481 slerafseri dleqieelvn vikkkedket qlskdlidkr ykieelseal resvviasds
      541 erklkneekt kkealqrlak leqriasmqt asalkcitcq pllsklrkye qklerlteer
      601 ttqledlhqm krealkaavs ekdahlalle lsgiktvaqs vqaeqlkadr krlldklkke
      661 deksinsste snpndseetp qlltkllets ddeetlndrr sdsssprpat rngdscghsk
      721 ktskgt
//
LOCUS       XP_076546171             562 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546171
VERSION     XP_076546171.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690056.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..562
                     /product="aldehyde dehydrogenase type III isoform X1"
                     /calculated_mol_wt=62394
     Region          66..511
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(118,148..149,153,160,164,166,241,243,251,255,258,
                     265,285,288,291..292,295,332,342,421..422,426,431,437,440,
                     443..452,458,463,466..467,469,472,486,488..489,491..495,
                     501,503..504,506..510)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(174..176,202,231,249,348,350..351,354,396,398)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(176,271,302,305)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..562
                     /gene="Aldh-III"
                     /coded_by="XM_076690056.1:16..1704"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mpfltrkhsn naevaidmpg nkeepktset egnestteec rieiqegvsn taesaysngk
       61 mvtdyaslve rtrntfftgk trplewrkkq lkqsllmlee ckseiisala sdlhrckfes
      121 iameiqiieg eikdmlmhik ewsadekppk amvnmldkve irkdpygivl vigpwnyplq
      181 lclipvigai aagncvilkp selslatsql lakilpryld secvhvvlgg vpettellkq
      241 kfdyifytgs tsvgkiirea ankfltpvtl elggkspvyi dntadisiav krilwgkcin
      301 vgqtciapdy iictpevqnk fipeaekvlk ewygenpkes pdlariisdn hyqrlvkyls
      361 gngkvaiggn cdpvdkyisp tilidvkptd pvmqdeifgp ilpfvtvnna yeaikfinsr
      421 ekplalyifs sdestislfl entssgdvcv ndvmmhaavd tlpfggvgys gmgcyhgkyt
      481 fdtfvhkkgc likdynklve siascryppy sdkklsflsl miakrpdipg ikyiphllaf
      541 gfgilvavgv saalkvykpk rl
//
LOCUS       XP_076546172             562 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546172
VERSION     XP_076546172.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690057.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..562
                     /product="aldehyde dehydrogenase type III isoform X2"
                     /calculated_mol_wt=62571
     Region          66..511
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(118,148..149,153,160,164,166,241,243,251,255,258,
                     265,285,288,291..292,295,332,342,421..422,426,431,437,440,
                     443..452,458,463,466..467,469,472,486,488..489,491..495,
                     501,503..504,506..510)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(174..176,202,231,249,348,350..351,354,396,398)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(176,271,302,305)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..562
                     /gene="Aldh-III"
                     /coded_by="XM_076690057.1:16..1704"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mpfltrkhsn naevaidmpg nkeepktset egnestteec rieiqegvsn taesaysngk
       61 mvtdyaslve rtrntfftgk trplewrkkq lkqsllmlee ckseiisala sdlhrckfes
      121 iameiqiieg eikdmlmhik ewsadekppk amvnmldkve irkdpygivl vigpwnyplq
      181 lclipvigai aagncvilkp selslatsql lakilpryld secvhvvlgg vpettellkq
      241 kfdyifytgs tsvgkiirea ankfltpvtl elggkspvyi dntadisiav krilwgkcin
      301 vgqtciapdy iictpevqnk fipeaekvlk ewygenpkes pdlariisdn hyqrlvkyls
      361 gngkvaiggn cdpvdkyisp tilidvkptd pvmqdeifgp ilpfvtvnna yeaikfinsr
      421 nsplvlyvft kdkrvqelii nqtqsgsvav netimqftvd tlpfggvgys gmgcyhgkyt
      481 fdtfvhkkgc likdynklve siascryppy sdkklsflsl miakrpdipg ikyiphllaf
      541 gfgilvavgv saalkvykpk rl
//
LOCUS       XP_076546173             561 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546173
VERSION     XP_076546173.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690058.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="aldehyde dehydrogenase type III isoform X3"
                     /calculated_mol_wt=62271
     Region          66..511
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(118,148..149,153,160,164,166,241,243,251,255,258,
                     265,285,288,291..292,295,332,342,421..422,426,431,437,440,
                     443..452,458,463,466..467,469,472,486,488..489,491..495,
                     501,503..504,506..510)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(174..176,202,231,249,348,350..351,354,396,398)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(176,271,302,305)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..561
                     /gene="Aldh-III"
                     /coded_by="XM_076690058.1:16..1701"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mpfltrkhsn naevaidmpg nkeepktset egnestteec rieiqegvsn taesaysngk
       61 mvtdyaslve rtrntfftgk trplewrkkq lkqsllmlee ckseiisala sdlhrckfes
      121 iameiqiieg eikdmlmhik ewsadekppk amvnmldkve irkdpygivl vigpwnyplq
      181 lclipvigai aagncvilkp selslatsql lakilpryld secvhvvlgg vpettellkq
      241 kfdyifytgs tsvgkiirea ankfltpvtl elggkspvyi dntadisiav krilwgkcin
      301 vgqtciapdy iictpevqnk fipeaekvlk ewygenpkes pdlariisdn hyqrlvkyls
      361 gngkvaiggn cdpvdkyisp tilidvkptd pvmqdeifgp ilpfvtvnna yeaikfinsr
      421 ekplalyifs sdestislfl entssgdvcv ndvmmhaavd tlpfggvgys gmgcyhgkyt
      481 fdtfvhkkgc likdynklve siascryppy sdkklsflsl miakrpdipg ikyiphllaf
      541 gfgilvavgv saalkvqeen y
//
LOCUS       XP_034173343             552 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173343
VERSION     XP_034173343.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317452.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..552
                     /product="aldehyde dehydrogenase type III isoform X4"
                     /calculated_mol_wt=61198
     Region          56..501
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(108,138..139,143,150,154,156,231,233,241,245,248,
                     255,275,278,281..282,285,322,332,411..412,416,421,427,430,
                     433..442,448,453,456..457,459,462,476,478..479,481..485,
                     491,493..494,496..500)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(164..166,192,221,239,338,340..341,344,386,388)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(166,261,292,295)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..552
                     /gene="Aldh-III"
                     /coded_by="XM_034317452.2:144..1802"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 maevaidmpg nkeepktset egnestteec rieiqegvsn taesaysngk mvtdyaslve
       61 rtrntfftgk trplewrkkq lkqsllmlee ckseiisala sdlhrckfes iameiqiieg
      121 eikdmlmhik ewsadekppk amvnmldkve irkdpygivl vigpwnyplq lclipvigai
      181 aagncvilkp selslatsql lakilpryld secvhvvlgg vpettellkq kfdyifytgs
      241 tsvgkiirea ankfltpvtl elggkspvyi dntadisiav krilwgkcin vgqtciapdy
      301 iictpevqnk fipeaekvlk ewygenpkes pdlariisdn hyqrlvkyls gngkvaiggn
      361 cdpvdkyisp tilidvkptd pvmqdeifgp ilpfvtvnna yeaikfinsr ekplalyifs
      421 sdestislfl entssgdvcv ndvmmhaavd tlpfggvgys gmgcyhgkyt fdtfvhkkgc
      481 likdynklve siascryppy sdkklsflsl miakrpdipg ikyiphllaf gfgilvavgv
      541 saalkvykpk rl
//
LOCUS       XP_034173368             545 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173368
VERSION     XP_034173368.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317477.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..545
                     /product="aldehyde dehydrogenase type III isoform X5"
                     /calculated_mol_wt=60468
     Region          49..494
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(101,131..132,136,143,147,149,224,226,234,238,241,
                     248,268,271,274..275,278,315,325,404..405,409,414,420,423,
                     426..435,441,446,449..450,452,455,469,471..472,474..478,
                     484,486..487,489..493)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(157..159,185,214,232,331,333..334,337,379,381)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(159,254,285,288)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..545
                     /gene="Aldh-III"
                     /coded_by="XM_034317477.2:113..1750"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mpgnkeepkt setegnestt eecrieiqeg vsntaesays ngkmvtdyas lvertrntff
       61 tgktrplewr kkqlkqsllm leeckseiis alasdlhrck fesiameiqi iegeikdmlm
      121 hikewsadek ppkamvnmld kveirkdpyg ivlvigpwny plqlclipvi gaiaagncvi
      181 lkpselslat sqllakilpr yldsecvhvv lggvpettel lkqkfdyify tgstsvgkii
      241 reaankfltp vtlelggksp vyidntadis iavkrilwgk cinvgqtcia pdyiictpev
      301 qnkfipeaek vlkewygenp kespdlarii sdnhyqrlvk ylsgngkvai ggncdpvdky
      361 isptilidvk ptdpvmqdei fgpilpfvtv nnayeaikfi nsrekplaly ifssdestis
      421 lflentssgd vcvndvmmha avdtlpfggv gysgmgcyhg kytfdtfvhk kgclikdynk
      481 lvesiascry ppysdkklsf lslmiakrpd ipgikyiphl lafgfgilva vgvsaalkvy
      541 kpkrl
//
LOCUS       XP_034173377             545 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173377
VERSION     XP_034173377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..545
                     /product="aldehyde dehydrogenase type III isoform X5"
                     /calculated_mol_wt=60468
     Region          49..494
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(101,131..132,136,143,147,149,224,226,234,238,241,
                     248,268,271,274..275,278,315,325,404..405,409,414,420,423,
                     426..435,441,446,449..450,452,455,469,471..472,474..478,
                     484,486..487,489..493)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(157..159,185,214,232,331,333..334,337,379,381)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(159,254,285,288)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..545
                     /gene="Aldh-III"
                     /coded_by="XM_034317486.2:37..1674"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mpgnkeepkt setegnestt eecrieiqeg vsntaesays ngkmvtdyas lvertrntff
       61 tgktrplewr kkqlkqsllm leeckseiis alasdlhrck fesiameiqi iegeikdmlm
      121 hikewsadek ppkamvnmld kveirkdpyg ivlvigpwny plqlclipvi gaiaagncvi
      181 lkpselslat sqllakilpr yldsecvhvv lggvpettel lkqkfdyify tgstsvgkii
      241 reaankfltp vtlelggksp vyidntadis iavkrilwgk cinvgqtcia pdyiictpev
      301 qnkfipeaek vlkewygenp kespdlarii sdnhyqrlvk ylsgngkvai ggncdpvdky
      361 isptilidvk ptdpvmqdei fgpilpfvtv nnayeaikfi nsrekplaly ifssdestis
      421 lflentssgd vcvndvmmha avdtlpfggv gysgmgcyhg kytfdtfvhk kgclikdynk
      481 lvesiascry ppysdkklsf lslmiakrpd ipgikyiphl lafgfgilva vgvsaalkvy
      541 kpkrl
//
LOCUS       XP_034173396             510 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173396
VERSION     XP_034173396.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="aldehyde dehydrogenase type III isoform X6"
                     /calculated_mol_wt=56689
     Region          14..459
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(66,96..97,101,108,112,114,189,191,199,203,206,213,
                     233,236,239..240,243,280,290,369..370,374,379,385,388,
                     391..400,406,411,414..415,417,420,434,436..437,439..443,
                     449,451..452,454..458)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(122..124,150,179,197,296,298..299,302,344,346)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(124,219,250,253)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..510
                     /gene="Aldh-III"
                     /coded_by="XM_034317505.2:106..1638"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mlaysngkmv tdyaslvert rntfftgktr plewrkkqlk qsllmleeck seiisalasd
       61 lhrckfesia meiqiiegei kdmlmhikew sadekppkam vnmldkveir kdpygivlvi
      121 gpwnyplqlc lipvigaiaa gncvilkpse lslatsqlla kilpryldse cvhvvlggvp
      181 ettellkqkf dyifytgsts vgkiireaan kfltpvtlel ggkspvyidn tadisiavkr
      241 ilwgkcinvg qtciapdyii ctpevqnkfi peaekvlkew ygenpkespd lariisdnhy
      301 qrlvkylsgn gkvaiggncd pvdkyispti lidvkptdpv mqdeifgpil pfvtvnnaye
      361 aikfinsrek plalyifssd estislflen tssgdvcvnd vmmhaavdtl pfggvgysgm
      421 gcyhgkytfd tfvhkkgcli kdynklvesi ascryppysd kklsflslmi akrpdipgik
      481 yiphllafgf gilvavgvsa alkvykpkrl
//
LOCUS       XP_034173407             502 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173407
VERSION     XP_034173407.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317516.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="aldehyde dehydrogenase type III isoform X7"
                     /calculated_mol_wt=55824
     Region          6..451
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(58,88..89,93,100,104,106,181,183,191,195,198,205,
                     225,228,231..232,235,272,282,361..362,366,371,377,380,
                     383..392,398,403,406..407,409,412,426,428..429,431..435,
                     441,443..444,446..450)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(114..116,142,171,189,288,290..291,294,336,338)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(116,211,242,245)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..502
                     /gene="Aldh-III"
                     /coded_by="XM_034317516.2:139..1647"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mvtdyaslve rtrntfftgk trplewrkkq lkqsllmlee ckseiisala sdlhrckfes
       61 iameiqiieg eikdmlmhik ewsadekppk amvnmldkve irkdpygivl vigpwnyplq
      121 lclipvigai aagncvilkp selslatsql lakilpryld secvhvvlgg vpettellkq
      181 kfdyifytgs tsvgkiirea ankfltpvtl elggkspvyi dntadisiav krilwgkcin
      241 vgqtciapdy iictpevqnk fipeaekvlk ewygenpkes pdlariisdn hyqrlvkyls
      301 gngkvaiggn cdpvdkyisp tilidvkptd pvmqdeifgp ilpfvtvnna yeaikfinsr
      361 ekplalyifs sdestislfl entssgdvcv ndvmmhaavd tlpfggvgys gmgcyhgkyt
      421 fdtfvhkkgc likdynklve siascryppy sdkklsflsl miakrpdipg ikyiphllaf
      481 gfgilvavgv saalkvykpk rl
//
LOCUS       XP_034173416             502 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173416
VERSION     XP_034173416.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317525.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="aldehyde dehydrogenase type III isoform X7"
                     /calculated_mol_wt=55824
     Region          6..451
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(58,88..89,93,100,104,106,181,183,191,195,198,205,
                     225,228,231..232,235,272,282,361..362,366,371,377,380,
                     383..392,398,403,406..407,409,412,426,428..429,431..435,
                     441,443..444,446..450)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(114..116,142,171,189,288,290..291,294,336,338)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(116,211,242,245)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..502
                     /gene="Aldh-III"
                     /coded_by="XM_034317525.2:135..1643"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mvtdyaslve rtrntfftgk trplewrkkq lkqsllmlee ckseiisala sdlhrckfes
       61 iameiqiieg eikdmlmhik ewsadekppk amvnmldkve irkdpygivl vigpwnyplq
      121 lclipvigai aagncvilkp selslatsql lakilpryld secvhvvlgg vpettellkq
      181 kfdyifytgs tsvgkiirea ankfltpvtl elggkspvyi dntadisiav krilwgkcin
      241 vgqtciapdy iictpevqnk fipeaekvlk ewygenpkes pdlariisdn hyqrlvkyls
      301 gngkvaiggn cdpvdkyisp tilidvkptd pvmqdeifgp ilpfvtvnna yeaikfinsr
      361 ekplalyifs sdestislfl entssgdvcv ndvmmhaavd tlpfggvgys gmgcyhgkyt
      421 fdtfvhkkgc likdynklve siascryppy sdkklsflsl miakrpdipg ikyiphllaf
      481 gfgilvavgv saalkvykpk rl
//
LOCUS       XP_034173387             545 aa            linear   INV 03-SEP-2025
DEFINITION  aldehyde dehydrogenase type III isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173387
VERSION     XP_034173387.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..545
                     /product="aldehyde dehydrogenase type III isoform X5"
                     /calculated_mol_wt=60468
     Region          49..494
                     /region_name="ALDH_F3AB"
                     /note="Aldehyde dehydrogenase family 3 members A1, A2, and
                     B1 and related proteins; cd07132"
                     /db_xref="CDD:143450"
     Site            order(101,131..132,136,143,147,149,224,226,234,238,241,
                     248,268,271,274..275,278,315,325,404..405,409,414,420,423,
                     426..435,441,446,449..450,452,455,469,471..472,474..478,
                     484,486..487,489..493)
                     /site_type="other"
                     /note="homodimeric interface [polypeptide binding]"
                     /db_xref="CDD:143450"
     Site            order(157..159,185,214,232,331,333..334,337,379,381)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:143450"
     Site            order(159,254,285,288)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:143450"
     CDS             1..545
                     /gene="Aldh-III"
                     /coded_by="XM_034317496.2:88..1725"
                     /db_xref="GeneID:117601100"
ORIGIN      
        1 mpgnkeepkt setegnestt eecrieiqeg vsntaesays ngkmvtdyas lvertrntff
       61 tgktrplewr kkqlkqsllm leeckseiis alasdlhrck fesiameiqi iegeikdmlm
      121 hikewsadek ppkamvnmld kveirkdpyg ivlvigpwny plqlclipvi gaiaagncvi
      181 lkpselslat sqllakilpr yldsecvhvv lggvpettel lkqkfdyify tgstsvgkii
      241 reaankfltp vtlelggksp vyidntadis iavkrilwgk cinvgqtcia pdyiictpev
      301 qnkfipeaek vlkewygenp kespdlarii sdnhyqrlvk ylsgngkvai ggncdpvdky
      361 isptilidvk ptdpvmqdei fgpilpfvtv nnayeaikfi nsrekplaly ifssdestis
      421 lflentssgd vcvndvmmha avdtlpfggv gysgmgcyhg kytfdtfvhk kgclikdynk
      481 lvesiascry ppysdkklsf lslmiakrpd ipgikyiphl lafgfgilva vgvsaalkvy
      541 kpkrl
//
LOCUS       XP_034196439             542 aa            linear   INV 03-SEP-2025
DEFINITION  beta-hexosaminidase subunit beta [Osmia lignaria lignaria].
ACCESSION   XP_034196439
VERSION     XP_034196439.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340548.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196439.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="beta-hexosaminidase subunit beta"
                     /calculated_mol_wt=61064
     Region          36..166
                     /region_name="Glycohydro_20b2"
                     /note="beta-acetyl hexosaminidase like; pfam14845"
                     /db_xref="CDD:434261"
     Region          190..528
                     /region_name="GH20_HexA_HexB-like"
                     /note="Beta-N-acetylhexosaminidases catalyze the removal
                     of beta-1,4-linked N-acetyl-D-hexosamine residues from the
                     non-reducing ends of N-acetyl-beta-D-hexosaminides
                     including N-acetylglucosides and N-acetylgalactosides. The
                     hexA and hexB genes encode the...; cd06562"
                     /db_xref="CDD:119332"
     Site            order(200,229,283,344..345,395,414,439,441,478,480)
                     /site_type="active"
                     /db_xref="CDD:119332"
     CDS             1..542
                     /gene="LOC117611979"
                     /coded_by="XM_034340548.2:79..1707"
                     /db_xref="GeneID:117611979"
ORIGIN      
        1 mstmrlvssl llllvvqtns lnpdhgpwvq atrgepwpyp dlrrvskafy llrastfqfn
       61 vigetcdivv daierykaii lteariariy sqghpelsrn ndtakgtlsa lnihlgepce
      121 kdgnhwphlq msesytlsin etstvanlva esvwgilrgl etfsqlitpa gdgsnlrirc
      181 qtivdrpklp hrgllldtsr hylpisaill tldamsfnkm nvlhwhivdd nsfpyqssky
      241 pnlsakgayh ptmvytlndi qkvvdyarlr girvmpefdt pghtrswgla ypdllttcyd
      301 srgrangqlg pmnpinpnly eflrhlfaei vqvfpdqyvh lggdevpfnc wmsnpeinay
      361 mkhrnmsrny alleaeyiak llqitnslea ntivwqevfd ngvkmpntta vhvwtgvwak
      421 eleeataagh pvllsacwyl dhiagggdwr kfyrcdplaf ngaahvshlm lggeacmwge
      481 fvdknnvhpr iwprasatae rlwssaklde nkaaqrleeh scrmnrrgip aqppngpgfc
      541 vm
//
LOCUS       XP_034173439             477 aa            linear   INV 03-SEP-2025
DEFINITION  COP9 signalosome subunit 1b [Osmia lignaria lignaria].
ACCESSION   XP_034173439
VERSION     XP_034173439.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317548.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173439.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="COP9 signalosome subunit 1b"
                     /calculated_mol_wt=54379
     Region          121..301
                     /region_name="RPN7"
                     /note="26S proteasome subunit RPN7; pfam10602"
                     /db_xref="CDD:431387"
     Region          316..420
                     /region_name="PCI"
                     /note="PCI domain; pfam01399"
                     /db_xref="CDD:460195"
     CDS             1..477
                     /gene="CSN1b"
                     /coded_by="XM_034317548.2:95..1528"
                     /db_xref="GeneID:117601145"
ORIGIN      
        1 mplqvhdvqq nvvepmqvda paedndnaee epyivenptl dlevyansyt glaklyrliy
       61 iadhcpmlri ealkmaisyv mitynvslyv ilhkklvqav gtpglpdvaa qstsqdmltl
      121 dqawvdsrsk kaalklekfd tdlknyrsns ikesirrghd dlgdhyldcg dlvhalkcys
      181 rardyctsgk hvvnmclnvi kvsvylqnwt hvlsyvtkae stpdfsdvhg kdnnqfiitk
      241 lkvaaglael atrkykmaar hflqasldhc dcpellspgn valygglcal atfdrqelqk
      301 qvifnssfkl flelepqlrd iifkfyesky asclklldei kdnilldmyi aphvnvlytk
      361 irnraliqyf spylsadmkr matafnrtvs eledelmqli ldgqiqarid shnkilyakd
      421 vdqrsttfek smsvgkeyqr rtrmlilraa mlkqqiqvks pqrdstqage msvnwkq
//
LOCUS       XP_034173489             263 aa            linear   INV 03-SEP-2025
DEFINITION  neuralized-like protein 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034173489
VERSION     XP_034173489.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317598.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="neuralized-like protein 2 isoform X1"
                     /calculated_mol_wt=28982
     Region          11..219
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          225..261
                     /region_name="SOCS_SOCS_like"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS-like proteins. The CIS/SOCS family of proteins is
                     characterized by the presence of a C-terminal SOCS box and
                     a central SH2 domain. These intracellular proteins
                     regulate the responses of immune cells to...; cd03717"
                     /db_xref="CDD:239687"
     Site            order(225..229,235,242,248)
                     /site_type="active"
                     /note="elongin B/C interaction [active]"
                     /db_xref="CDD:239687"
     CDS             1..263
                     /gene="LOC117601164"
                     /coded_by="XM_034317598.2:437..1228"
                     /db_xref="GeneID:117601164"
ORIGIN      
        1 mteksrkivt rfhpthgeni vlredstvay rtasfansla fsekplqpge iflveiekte
       61 rgwsghmrlg ltlldpasvs nslpqfampn lvrlgntwif aitkshtiwd sfegygegip
      121 sgekklitdg vnvqtsrgvi pfnalrpnii dpsqyilptd agsrigimyv pqagsdkaem
      181 hfiingedqg vcgkdipyka gplravvdvy gttkqvrivq lygavstlqs acrdailqyt
      241 krnavdslpl prvlkdylly qsq
//
LOCUS       XP_034173497             262 aa            linear   INV 03-SEP-2025
DEFINITION  neuralized-like protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034173497
VERSION     XP_034173497.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317606.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..262
                     /product="neuralized-like protein 2 isoform X2"
                     /calculated_mol_wt=28911
     Region          11..219
                     /region_name="SPRY_NHR_like"
                     /note="SPRY domain in neuralized homology repeat; cd12887"
                     /db_xref="CDD:293945"
     Region          222..260
                     /region_name="SOCS_SOCS_like"
                     /note="SOCS (suppressors of cytokine signaling) box of
                     SOCS-like proteins. The CIS/SOCS family of proteins is
                     characterized by the presence of a C-terminal SOCS box and
                     a central SH2 domain. These intracellular proteins
                     regulate the responses of immune cells to...; cd03717"
                     /db_xref="CDD:239687"
     Site            order(223..228,234,241,247)
                     /site_type="active"
                     /note="elongin B/C interaction [active]"
                     /db_xref="CDD:239687"
     CDS             1..262
                     /gene="LOC117601164"
                     /coded_by="XM_034317606.2:434..1222"
                     /db_xref="GeneID:117601164"
ORIGIN      
        1 mteksrkivt rfhpthgeni vlredstvay rtasfansla fsekplqpge iflveiekte
       61 rgwsghmrlg ltlldpasvs nslpqfampn lvrlgntwif aitkshtiwd sfegygegip
      121 sgekklitdg vnvqtsrgvi pfnalrpnii dpsqyilptd agsrigimyv pqagsdkaem
      181 hfiingedqg vcgkdipyka gplravvdvy gttkqvrivq lygvstlqsa crdailqytk
      241 rnavdslplp rvlkdyllyq sq
//
LOCUS       XP_034173521             123 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601187 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173521
VERSION     XP_034173521.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317630.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="uncharacterized protein LOC117601187 isoform X1"
                     /calculated_mol_wt=12781
     CDS             1..123
                     /gene="LOC117601187"
                     /coded_by="XM_034317630.2:456..827"
                     /db_xref="GeneID:117601187"
ORIGIN      
        1 mlkgctgsis ecqsccsscc tpspcsptvi crpscpprcp pkptrttvyl rpcppcppcc
       61 plpcppkiiv tctpppppcp ppppcvtyvc tkklvpcmpc ppptpppcsp crpvclpysc
      121 spc
//
LOCUS       XP_034173531              75 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601187 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173531
VERSION     XP_034173531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317640.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..75
                     /product="uncharacterized protein LOC117601187 isoform X2"
                     /calculated_mol_wt=7882
     CDS             1..75
                     /gene="LOC117601187"
                     /coded_by="XM_034317640.2:456..683"
                     /db_xref="GeneID:117601187"
ORIGIN      
        1 mlkgctgsis ecqsccsscc tpspcsptvi crpscpprcp pkptrttcpi avhlargpfl
       61 rqrdntvdii ilgih
//
LOCUS       XP_034171174             136 aa            linear   INV 03-SEP-2025
DEFINITION  histone H3 [Osmia lignaria lignaria].
ACCESSION   XP_034171174
VERSION     XP_034171174.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034315283.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="histone H3"
                     /calculated_mol_wt=15257
     Region          1..136
                     /region_name="PTZ00018"
                     /note="histone H3; Provisional"
                     /db_xref="CDD:185400"
     Site            order(40..48,50,64..67,70,73,84..87,117..119,121)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:467036"
     Site            order(45,48,51..52,55..56,58,60,62..63,67..68,70..72,
                     74..75,77,79,82..86,88..89,92..93,96..98,100..102,
                     104..106,109,118..122,126,129)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     Site            order(49,52..53,56..57,59,95,99,102,109,113,118)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     Site            order(107,110..111,114..115,117,123..124,127..128,
                     130..132)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:467036"
     CDS             1..136
                     /gene="LOC117600182"
                     /coded_by="XM_034315283.2:31..441"
                     /db_xref="GeneID:117600182"
ORIGIN      
        1 martkqtark stggkaprkq latkaarksa patggvkkph ryrpgtvalr eirryqkste
       61 llirklpfqr lvreiaqdfk tdlrfqssav malqeaseay lvglfedtnl caihakrvti
      121 mpkdiqlarr irgera
//
LOCUS       XP_034172221             103 aa            linear   INV 03-SEP-2025
DEFINITION  histone H4 [Osmia lignaria lignaria].
ACCESSION   XP_034172221
VERSION     XP_034172221.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316330.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..103
                     /product="histone H4"
                     /calculated_mol_wt=11250
     Region          1..103
                     /region_name="PLN00035"
                     /note="histone H4; Provisional"
                     /db_xref="CDD:177669"
     CDS             1..103
                     /gene="LOC117600627"
                     /coded_by="XM_034316330.2:70..381"
                     /db_xref="GeneID:117600627"
ORIGIN      
        1 mtgrgkggkg lgkggakrhr kvlrdniqgi tkpairrlar rggvkrisgl iyeetrgvlk
       61 vflenvirda vtytehakrk tvtamdvvya lkrqgrtlyg fgg
//
LOCUS       XP_034175785             831 aa            linear   INV 03-SEP-2025
DEFINITION  glycogen binding subunit 76A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175785
VERSION     XP_034175785.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175785.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="glycogen binding subunit 76A isoform X1"
                     /calculated_mol_wt=92789
     Region          <580..621
                     /region_name="PBD_PPP1R3"
                     /note="PP1C binding domain found in protein phosphatase 1
                     regulatory subunit 3 (PPP1R3) family; cl41685"
                     /db_xref="CDD:455118"
     Site            order(603..609,611,613,615..621)
                     /site_type="other"
                     /note="PP1C binding site [polypeptide binding]"
                     /db_xref="CDD:439278"
     Region          698..800
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..831
                     /gene="Gbs-76A"
                     /coded_by="XM_034319894.2:134..2629"
                     /db_xref="GeneID:117602192"
ORIGIN      
        1 misivyilse iirrlcdril lscvketrys swvkantesr eseeqgsstt spsegrhadg
       61 tsgssvlvmg stdsagsahs ggpscglvss lfpsscrgra eafarrlhrr ltslgsedas
      121 qrnkdptnpk etswlrssst tksmvnnast nhvlqhqprh npdtelcydf dlsdlcqdfe
      181 dglsspaeea taaemadlvv npealknhrd lrhgspngsk tmyssldses gyvyasplme
      241 rspdsdssdi yfdpessdve kaknevyfdp vttsdsemtt snlsngcept kmknvsyrwr
      301 petkmeqsed tsestssmpr nsfsneesqn nelmcnskds nerssletts lsheslwstf
      361 destmslqde spsrissglt afpnrlpksh sdseipthgp rlviscewdk vdqgvdevdf
      421 vnglsengkl edmknvrnle mkeestdsse ntteeelpyd eekpsvsvvq qnnlslkvee
      481 navetgigst yssminipga ltleyptger vieatvknev qlqiengsva nssdedkmcl
      541 llkklttdst akddvqeatq epqgnieeee egeeeeevrp qkirrssslk tgktppgtpg
      601 rkkivrfadv lgldladvrt fldeipkipn saysdliydd ifqkdsspvt sipniwgdry
      661 tesrrdssys lpprkldrtl mplfqqpggl pnfletvrer rvclenvlvq dpismciqgt
      721 vrvinldfhk svhirytlns wrnfsdlqai yvpnscdgfs dkfsfvlych tlpvgqrlef
      781 avrfqckgeq ywdnnagany cfqclptssa idyipitaqe nqhhewspmf y
//
LOCUS       XP_034175795             824 aa            linear   INV 03-SEP-2025
DEFINITION  glycogen binding subunit 76A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034175795
VERSION     XP_034175795.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319904.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175795.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..824
                     /product="glycogen binding subunit 76A isoform X2"
                     /calculated_mol_wt=91744
     Region          <573..614
                     /region_name="PBD_PPP1R3"
                     /note="PP1C binding domain found in protein phosphatase 1
                     regulatory subunit 3 (PPP1R3) family; cl41685"
                     /db_xref="CDD:455118"
     Site            order(596..602,604,606,608..614)
                     /site_type="other"
                     /note="PP1C binding site [polypeptide binding]"
                     /db_xref="CDD:439278"
     Region          691..793
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..824
                     /gene="Gbs-76A"
                     /coded_by="XM_034319904.2:982..3456"
                     /db_xref="GeneID:117602192"
ORIGIN      
        1 mimthteeas ttfesktptd qmnghsmant esreseeqgs sttspsegrh adgtsgssvl
       61 vmgstdsags ahsggpscgl vsslfpsscr graeafarrl hrrltslgse dasqrnkdpt
      121 npketswlrs ssttksmvnn astnhvlqhq prhnpdtelc ydfdlsdlcq dfedglsspa
      181 eeataaemad lvvnpealkn hrdlrhgspn gsktmyssld sesgyvyasp lmerspdsds
      241 sdiyfdpess dvekaknevy fdpvttsdse mttsnlsngc eptkmknvsy rwrpetkmeq
      301 sedtsestss mprnsfsnee sqnnelmcns kdsnerssle ttslsheslw stfdestmsl
      361 qdespsriss gltafpnrlp kshsdseipt hgprlvisce wdkvdqgvde vdfvnglsen
      421 gkledmknvr nlemkeestd ssentteeel pydeekpsvs vvqqnnlslk veenavetgi
      481 gstyssmini pgaltleypt gervieatvk nevqlqieng svanssdedk mclllkkltt
      541 dstakddvqe atqepqgnie eeeegeeeee vrpqkirrss slktgktppg tpgrkkivrf
      601 advlgldlad vrtfldeipk ipnsaysdli yddifqkdss pvtsipniwg drytesrrds
      661 syslpprkld rtlmplfqqp gglpnfletv rerrvclenv lvqdpismci qgtvrvinld
      721 fhksvhiryt lnswrnfsdl qaiyvpnscd gfsdkfsfvl ychtlpvgqr lefavrfqck
      781 geqywdnnag anycfqclpt ssaidyipit aqenqhhews pmfy
//
LOCUS       XP_034175805             763 aa            linear   INV 03-SEP-2025
DEFINITION  glycogen binding subunit 76A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034175805
VERSION     XP_034175805.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319914.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175805.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..763
                     /product="glycogen binding subunit 76A isoform X3"
                     /calculated_mol_wt=85299
     Region          <512..553
                     /region_name="PBD_PPP1R3"
                     /note="PP1C binding domain found in protein phosphatase 1
                     regulatory subunit 3 (PPP1R3) family; cl41685"
                     /db_xref="CDD:455118"
     Site            order(535..541,543,545,547..553)
                     /site_type="other"
                     /note="PP1C binding site [polypeptide binding]"
                     /db_xref="CDD:439278"
     Region          630..732
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..763
                     /gene="Gbs-76A"
                     /coded_by="XM_034319914.2:403..2694"
                     /db_xref="GeneID:117602192"
ORIGIN      
        1 mgstdsagsa hsggpscglv sslfpsscrg raeafarrlh rrltslgsed asqrnkdptn
       61 pketswlrss sttksmvnna stnhvlqhqp rhnpdtelcy dfdlsdlcqd fedglsspae
      121 eataaemadl vvnpealknh rdlrhgspng sktmysslds esgyvyaspl merspdsdss
      181 diyfdpessd vekaknevyf dpvttsdsem ttsnlsngce ptkmknvsyr wrpetkmeqs
      241 edtsestssm prnsfsnees qnnelmcnsk dsnersslet tslsheslws tfdestmslq
      301 despsrissg ltafpnrlpk shsdseipth gprlviscew dkvdqgvdev dfvnglseng
      361 kledmknvrn lemkeestds sentteeelp ydeekpsvsv vqqnnlslkv eenavetgig
      421 styssminip galtleyptg ervieatvkn evqlqiengs vanssdedkm clllkklttd
      481 stakddvqea tqepqgniee eeegeeeeev rpqkirrsss lktgktppgt pgrkkivrfa
      541 dvlgldladv rtfldeipki pnsaysdliy ddifqkdssp vtsipniwgd rytesrrdss
      601 yslpprkldr tlmplfqqpg glpnfletvr errvclenvl vqdpismciq gtvrvinldf
      661 hksvhirytl nswrnfsdlq aiyvpnscdg fsdkfsfvly chtlpvgqrl efavrfqckg
      721 eqywdnnaga nycfqclpts saidyipita qenqhhewsp mfy
//
LOCUS       XP_034175814             763 aa            linear   INV 03-SEP-2025
DEFINITION  glycogen binding subunit 76A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034175814
VERSION     XP_034175814.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319923.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175814.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..763
                     /product="glycogen binding subunit 76A isoform X3"
                     /calculated_mol_wt=85299
     Region          <512..553
                     /region_name="PBD_PPP1R3"
                     /note="PP1C binding domain found in protein phosphatase 1
                     regulatory subunit 3 (PPP1R3) family; cl41685"
                     /db_xref="CDD:455118"
     Site            order(535..541,543,545,547..553)
                     /site_type="other"
                     /note="PP1C binding site [polypeptide binding]"
                     /db_xref="CDD:439278"
     Region          630..732
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..763
                     /gene="Gbs-76A"
                     /coded_by="XM_034319923.2:253..2544"
                     /db_xref="GeneID:117602192"
ORIGIN      
        1 mgstdsagsa hsggpscglv sslfpsscrg raeafarrlh rrltslgsed asqrnkdptn
       61 pketswlrss sttksmvnna stnhvlqhqp rhnpdtelcy dfdlsdlcqd fedglsspae
      121 eataaemadl vvnpealknh rdlrhgspng sktmysslds esgyvyaspl merspdsdss
      181 diyfdpessd vekaknevyf dpvttsdsem ttsnlsngce ptkmknvsyr wrpetkmeqs
      241 edtsestssm prnsfsnees qnnelmcnsk dsnersslet tslsheslws tfdestmslq
      301 despsrissg ltafpnrlpk shsdseipth gprlviscew dkvdqgvdev dfvnglseng
      361 kledmknvrn lemkeestds sentteeelp ydeekpsvsv vqqnnlslkv eenavetgig
      421 styssminip galtleyptg ervieatvkn evqlqiengs vanssdedkm clllkklttd
      481 stakddvqea tqepqgniee eeegeeeeev rpqkirrsss lktgktppgt pgrkkivrfa
      541 dvlgldladv rtfldeipki pnsaysdliy ddifqkdssp vtsipniwgd rytesrrdss
      601 yslpprkldr tlmplfqqpg glpnfletvr errvclenvl vqdpismciq gtvrvinldf
      661 hksvhirytl nswrnfsdlq aiyvpnscdg fsdkfsfvly chtlpvgqrl efavrfqckg
      721 eqywdnnaga nycfqclpts saidyipita qenqhhewsp mfy
//
LOCUS       XP_034175824             763 aa            linear   INV 03-SEP-2025
DEFINITION  glycogen binding subunit 76A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034175824
VERSION     XP_034175824.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319933.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175824.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..763
                     /product="glycogen binding subunit 76A isoform X3"
                     /calculated_mol_wt=85299
     Region          <512..553
                     /region_name="PBD_PPP1R3"
                     /note="PP1C binding domain found in protein phosphatase 1
                     regulatory subunit 3 (PPP1R3) family; cl41685"
                     /db_xref="CDD:455118"
     Site            order(535..541,543,545,547..553)
                     /site_type="other"
                     /note="PP1C binding site [polypeptide binding]"
                     /db_xref="CDD:439278"
     Region          630..732
                     /region_name="CBM_21"
                     /note="Carbohydrate/starch-binding module (family 21);
                     pfam03370"
                     /db_xref="CDD:427265"
     CDS             1..763
                     /gene="Gbs-76A"
                     /coded_by="XM_034319933.2:225..2516"
                     /db_xref="GeneID:117602192"
ORIGIN      
        1 mgstdsagsa hsggpscglv sslfpsscrg raeafarrlh rrltslgsed asqrnkdptn
       61 pketswlrss sttksmvnna stnhvlqhqp rhnpdtelcy dfdlsdlcqd fedglsspae
      121 eataaemadl vvnpealknh rdlrhgspng sktmysslds esgyvyaspl merspdsdss
      181 diyfdpessd vekaknevyf dpvttsdsem ttsnlsngce ptkmknvsyr wrpetkmeqs
      241 edtsestssm prnsfsnees qnnelmcnsk dsnersslet tslsheslws tfdestmslq
      301 despsrissg ltafpnrlpk shsdseipth gprlviscew dkvdqgvdev dfvnglseng
      361 kledmknvrn lemkeestds sentteeelp ydeekpsvsv vqqnnlslkv eenavetgig
      421 styssminip galtleyptg ervieatvkn evqlqiengs vanssdedkm clllkklttd
      481 stakddvqea tqepqgniee eeegeeeeev rpqkirrsss lktgktppgt pgrkkivrfa
      541 dvlgldladv rtfldeipki pnsaysdliy ddifqkdssp vtsipniwgd rytesrrdss
      601 yslpprkldr tlmplfqqpg glpnfletvr errvclenvl vqdpismciq gtvrvinldf
      661 hksvhirytl nswrnfsdlq aiyvpnscdg fsdkfsfvly chtlpvgqrl efavrfqckg
      721 eqywdnnaga nycfqclpts saidyipita qenqhhewsp mfy
//
LOCUS       XP_034175844             794 aa            linear   INV 03-SEP-2025
DEFINITION  small G protein signaling modulator 3 homolog isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034175844
VERSION     XP_034175844.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319953.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175844.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..794
                     /product="small G protein signaling modulator 3 homolog
                     isoform X1"
                     /calculated_mol_wt=91036
     Region          154..368
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          531..583
                     /region_name="SH3_SGSM3"
                     /note="Src Homology 3 domain of Small G protein Signaling
                     Modulator 3; cd11813"
                     /db_xref="CDD:212747"
     Site            order(536,538,541,545,563..564,577,579..580)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212747"
     Region          561..774
                     /region_name="RUN_SGSM3"
                     /note="RUN domain found in small G protein signaling
                     modulator 3 (SGSM3) and similar proteins; cd17688"
                     /db_xref="CDD:439050"
     CDS             1..794
                     /gene="LOC117602219"
                     /coded_by="XM_034319953.2:200..2584"
                     /db_xref="GeneID:117602219"
ORIGIN      
        1 mdiakslfnv rdhegyvgke dhnkkletav sedefemeit glcgeilspa pggsffaltp
       61 smwpqdilak lnqpddpnsq peyrfdefgf rveeedgpeq sskkllgipf vedpqhrlqw
      121 vallefshsk evaelswqnm drrlprtdkl rdmvrhgvph slrpqiwmrm sgalqkksss
      181 eitykdiika snndalmtnk qiekdllrim psnacfshlh stgiprlrrv mralawlypd
      241 igycqgtgti aaslllllee edafwmmati vedllpasyy sstligiqad qrvlrtlvsn
      301 ylphidqvli qhdielslis lhwfltlfas vvhmkillri wdmflfdgsi vlfqvtlgml
      361 kikeseliql ensaqifnal sdipgdiddv dqlfnaslev sgsltdvlie thrrrdlayl
      421 madqgglvgn pdavpnlpkq hlnrrqmkkn ksmlqtflfg nseteedaks knirqteilv
      481 dlreavlqla rhfinvdpkl sdivliadys mdshgkdhdn yinvsrtrkr rakalldfer
      541 hdddelgfrk ndiitiisqk dehcwvgeln glrgwfpakf vellderskq ytcagddtvs
      601 evvtdlvrgt lcpaikavle hgmrkpsflg gpchpwlfie easnrevekd fnsvysrlvl
      661 cktyrldedg kvltpeelly rcvqsvnlth dnahaqmdvk lrsliclgln eqvlhlwlev
      721 lcscvevvqk wyqpwsfiys pgwvqikcel rilsqfafnl npdwelppkk eqsqplkdgv
      781 rdmlvkhhlf swdl
//
LOCUS       XP_076546174             728 aa            linear   INV 03-SEP-2025
DEFINITION  small G protein signaling modulator 3 homolog isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546174
VERSION     XP_076546174.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690059.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="small G protein signaling modulator 3 homolog
                     isoform X2"
                     /calculated_mol_wt=83781
     Region          88..302
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          465..517
                     /region_name="SH3_SGSM3"
                     /note="Src Homology 3 domain of Small G protein Signaling
                     Modulator 3; cd11813"
                     /db_xref="CDD:212747"
     Site            order(470,472,475,479,497..498,511,513..514)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212747"
     Region          495..708
                     /region_name="RUN_SGSM3"
                     /note="RUN domain found in small G protein signaling
                     modulator 3 (SGSM3) and similar proteins; cd17688"
                     /db_xref="CDD:439050"
     CDS             1..728
                     /gene="LOC117602219"
                     /coded_by="XM_076690059.1:139..2325"
                     /db_xref="GeneID:117602219"
ORIGIN      
        1 milihnqnig iclieyvrfd efgfrveeed gpeqsskkll gipfvedpqh rlqwvallef
       61 shskevaels wqnmdrrlpr tdklrdmvrh gvphslrpqi wmrmsgalqk kssseitykd
      121 iikasnndal mtnkqiekdl lrimpsnacf shlhstgipr lrrvmralaw lypdigycqg
      181 tgtiaaslll lleeedafwm mativedllp asyysstlig iqadqrvlrt lvsnylphid
      241 qvliqhdiel slislhwflt lfasvvhmki llriwdmflf dgsivlfqvt lgmlkikese
      301 liqlensaqi fnalsdipgd iddvdqlfna slevsgsltd vliethrrrd laylmadqgg
      361 lvgnpdavpn lpkqhlnrrq mkknksmlqt flfgnsetee daksknirqt eilvdlreav
      421 lqlarhfinv dpklsdivli adysmdshgk dhdnyinvsr trkrrakall dferhdddel
      481 gfrkndiiti isqkdehcwv gelnglrgwf pakfvellde rskqytcagd dtvsevvtdl
      541 vrgtlcpaik avlehgmrkp sflggpchpw lfieeasnre vekdfnsvys rlvlcktyrl
      601 dedgkvltpe ellyrcvqsv nlthdnahaq mdvklrslic lglneqvlhl wlevlcscve
      661 vvqkwyqpws fiyspgwvqi kcelrilsqf afnlnpdwel ppkkeqsqpl kdgvrdmlvk
      721 hhlfswdl
//
LOCUS       XP_034175857             732 aa            linear   INV 03-SEP-2025
DEFINITION  glycerate kinase [Osmia lignaria lignaria].
ACCESSION   XP_034175857
VERSION     XP_034175857.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319966.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175857.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..732
                     /product="glycerate kinase"
                     /calculated_mol_wt=82882
     Region          42..630
                     /region_name="GckA"
                     /note="Glycerate-2-kinase [Carbohydrate transport and
                     metabolism]; COG2379"
                     /db_xref="CDD:441946"
     CDS             1..732
                     /gene="LOC117602226"
                     /coded_by="XM_034319966.2:225..2423"
                     /db_xref="GeneID:117602226"
ORIGIN      
        1 mqcknilhkf tqysnpvffk fnlsninqft hrrmstdiks leetksilkk lyfagveavs
       61 pktlilnkvk fkngvlyagd qsfelkenvy ligfgkavmg maiilehmlg nylkkgiisv
      121 psastnamwe sedktyfpkl ttsvveyreg ginnqpddrc wetthdiidl aesltendtl
      181 ivlisgggsa llymprptid rddklelckk lqnagadike lnivrrklsm vkggglarma
      241 ypasvitlil sdivgdpvdl iasgptvysn kipkevinvl kkynlfqsle gdlkkiilak
      301 ekfkdrklit rkkkefkhvn niilgnnlva vegaafetyr nqltpiilrt dvagdvrdvs
      361 layahitsli clvldkkler eeffakvkdi pilslppakv deiynlienv sgeglvligg
      421 geptvvvkge gkggrnqela lhfsldwlak iksnprfaey nvvmlsagtd gqdgptdaag
      481 afgypaigpi ihniyqkakh ltsevvikae mkkeaikkke eekqlleslr pkeicktqll
      541 sgffpkkrte eqseikdeti earkkldsii deetllpfvy ltleiermlp qnalkendsy
      601 nlysrfkkga dllktgftgt nvmdlhfiyi kqrkceckid fekeisendl nmhdlyidps
      661 tiekykrmre lgmfkwqkgf yrssrvskde aeqlnlriid dnlldpccrk erkfpianvl
      721 kdkkvpvsye yp
//
LOCUS       XP_034175866             631 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602234 [Osmia lignaria lignaria].
ACCESSION   XP_034175866
VERSION     XP_034175866.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319975.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="uncharacterized protein LOC117602234"
                     /calculated_mol_wt=71034
     Region          <18..101
                     /region_name="PLN03150"
                     /note="hypothetical protein; Provisional"
                     /db_xref="CDD:178695"
     Region          42..65
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          64..123
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          66..89
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          90..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <275..564
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          277..299
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          300..322
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          323..345
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          346..368
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          369..392
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          393..416
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          417..440
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          441..464
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          465..502
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          503..535
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          536..559
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          558..620
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          560..583
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          584..605
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          608..630
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..631
                     /gene="LOC117602234"
                     /coded_by="XM_034319975.2:1..1896"
                     /db_xref="GeneID:117602234"
ORIGIN      
        1 mllpilflpl vvavplkssl snyiididng ilknvqfkge avneldlsgl girsleknsl
       61 dnvpnlksls lannslqslp efmfsnltnl eylslaenrl ssleylfirl eklrvlnisc
      121 npvmhlrrgh lfgltkstti ltegnslwsi stgvftnsfl kdeeelkhle kikaeadiei
      181 qndepekdls lvtennalni ndyipsqtkl itvnklklcm pekivlsiep leddktvsng
      241 cielpinekd rslnlrglgi kgfhegwyql qylpivsldl anneiteitk ellndlpegl
      301 iyvnlignri rgiwsqvien rylkmlnlrn nliekveega fqktnltalf lngnqledls
      361 fasslpqtlt elvlsgnqis sisseafsal srlvylnlan nkisklqddv fkgltslqvl
      421 iitrnsmtei epkafcdlkq lttlylhrns ltelqkgtfs eleslkdlnl awnklvritk
      481 dtfanlpptl dflhldfnei nalekgsfln vprftlsltg nkisdiprga fdlptlrdlh
      541 lnnntlttid gdsyeglpql krlwlnenqi reipkgsckn lgslnildis rnpfqklqng
      601 alyglssirg nflyiynnql nelqggifed i
//
LOCUS       XP_034175879             409 aa            linear   INV 03-SEP-2025
DEFINITION  divergent protein kinase domain 1C [Osmia lignaria lignaria].
ACCESSION   XP_034175879
VERSION     XP_034175879.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175879.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="divergent protein kinase domain 1C"
                     /calculated_mol_wt=46767
     Region          5..159
                     /region_name="PIP49_N"
                     /note="N-term cysteine-rich ER, FAM69; pfam14875"
                     /db_xref="CDD:464353"
     Region          178..374
                     /region_name="PIP49_C"
                     /note="Protein-kinase domain of FAM69; pfam12260"
                     /db_xref="CDD:463512"
     CDS             1..409
                     /gene="aln"
                     /coded_by="XM_034319988.2:1..1230"
                     /db_xref="GeneID:117602242"
ORIGIN      
        1 mnvrrlpgfm yhyrmvtlfl fsifllviyl llhwgimctn leawrhvsnv csthrkgtai
       61 gilceplcae rgihslacet lhagkeavfs ahwettrlmf kasrtkaqse qfeslfwtdi
      121 sgakhypset dfgtmikdlv vsklnvtlsq hqlerlmrlr thrvetestr rqlemenlwp
      181 llqeneylms ilyedrdifp qligtcgtfy aveyvrpiet pttvlalsds kpewakrlkl
      241 avmildllee ldtnfpepih lcdvkinhfg lplggqklkf ldldavfprt iinritadrk
      301 scekhedcdf fdcrsfcskn krcespvann nlqivcekif lgwtlsgtii ipgllmseht
      361 tstlavllrq canpagdveh lpraavsdsl ktrlynmlhe meqevaasv
//
LOCUS       XP_034175912             397 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 2-O-sulfotransferase pipe [Osmia lignaria
            lignaria].
ACCESSION   XP_034175912
VERSION     XP_034175912.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="heparan sulfate 2-O-sulfotransferase pipe"
                     /calculated_mol_wt=46159
     Region          109..371
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; cl22970"
                     /db_xref="CDD:451469"
     CDS             1..397
                     /gene="pip"
                     /coded_by="XM_034320021.2:2088..3281"
                     /db_xref="GeneID:117602248"
ORIGIN      
        1 mlndnvwkhr rvpvpkrtie lvalvaisst lflflhtrel hsrlrkmevr lqpgedeils
       61 anqlssegvq tdsspsgivh ypsghrpfav kykelevldi dalnntkrag rkvlffnrvp
      121 kvgsqtfmel lrrlsmrngf sfnrdrvqrv etirlapieq lqlarmvsgy aepsvyikhv
      181 cftnftefnl pqpiyinivr dpverviswy yyvrapwyyv erkqifpdlp lpdpnwlkkd
      241 fescvlkadr ecrylegeih egigdhrrqt lffcghsekc tpfntvgale rakmavekhy
      301 avvgvledvn atltvlenyi pgffqgatdv yydevnaftr inrnffkppv seevkdmvrs
      361 nftreiefyq fckqrlhmql ralkltkktp lsgsnsl
//
LOCUS       XP_076546176             397 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 2-O-sulfotransferase pipe [Osmia lignaria
            lignaria].
ACCESSION   XP_076546176
VERSION     XP_076546176.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690061.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="heparan sulfate 2-O-sulfotransferase pipe"
                     /calculated_mol_wt=46159
     Region          109..371
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; cl22970"
                     /db_xref="CDD:451469"
     CDS             1..397
                     /gene="pip"
                     /coded_by="XM_076690061.1:2040..3233"
                     /db_xref="GeneID:117602248"
ORIGIN      
        1 mlndnvwkhr rvpvpkrtie lvalvaisst lflflhtrel hsrlrkmevr lqpgedeils
       61 anqlssegvq tdsspsgivh ypsghrpfav kykelevldi dalnntkrag rkvlffnrvp
      121 kvgsqtfmel lrrlsmrngf sfnrdrvqrv etirlapieq lqlarmvsgy aepsvyikhv
      181 cftnftefnl pqpiyinivr dpverviswy yyvrapwyyv erkqifpdlp lpdpnwlkkd
      241 fescvlkadr ecrylegeih egigdhrrqt lffcghsekc tpfntvgale rakmavekhy
      301 avvgvledvn atltvlenyi pgffqgatdv yydevnaftr inrnffkppv seevkdmvrs
      361 nftreiefyq fckqrlhmql ralkltkktp lsgsnsl
//
LOCUS       XP_034175892             397 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 2-O-sulfotransferase pipe [Osmia lignaria
            lignaria].
ACCESSION   XP_034175892
VERSION     XP_034175892.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320001.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="heparan sulfate 2-O-sulfotransferase pipe"
                     /calculated_mol_wt=46159
     Region          109..371
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; cl22970"
                     /db_xref="CDD:451469"
     CDS             1..397
                     /gene="pip"
                     /coded_by="XM_034320001.2:1377..2570"
                     /db_xref="GeneID:117602248"
ORIGIN      
        1 mlndnvwkhr rvpvpkrtie lvalvaisst lflflhtrel hsrlrkmevr lqpgedeils
       61 anqlssegvq tdsspsgivh ypsghrpfav kykelevldi dalnntkrag rkvlffnrvp
      121 kvgsqtfmel lrrlsmrngf sfnrdrvqrv etirlapieq lqlarmvsgy aepsvyikhv
      181 cftnftefnl pqpiyinivr dpverviswy yyvrapwyyv erkqifpdlp lpdpnwlkkd
      241 fescvlkadr ecrylegeih egigdhrrqt lffcghsekc tpfntvgale rakmavekhy
      301 avvgvledvn atltvlenyi pgffqgatdv yydevnaftr inrnffkppv seevkdmvrs
      361 nftreiefyq fckqrlhmql ralkltkktp lsgsnsl
//
LOCUS       XP_076546177             397 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 2-O-sulfotransferase pipe [Osmia lignaria
            lignaria].
ACCESSION   XP_076546177
VERSION     XP_076546177.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690062.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="heparan sulfate 2-O-sulfotransferase pipe"
                     /calculated_mol_wt=46159
     Region          109..371
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; cl22970"
                     /db_xref="CDD:451469"
     CDS             1..397
                     /gene="pip"
                     /coded_by="XM_076690062.1:1338..2531"
                     /db_xref="GeneID:117602248"
ORIGIN      
        1 mlndnvwkhr rvpvpkrtie lvalvaisst lflflhtrel hsrlrkmevr lqpgedeils
       61 anqlssegvq tdsspsgivh ypsghrpfav kykelevldi dalnntkrag rkvlffnrvp
      121 kvgsqtfmel lrrlsmrngf sfnrdrvqrv etirlapieq lqlarmvsgy aepsvyikhv
      181 cftnftefnl pqpiyinivr dpverviswy yyvrapwyyv erkqifpdlp lpdpnwlkkd
      241 fescvlkadr ecrylegeih egigdhrrqt lffcghsekc tpfntvgale rakmavekhy
      301 avvgvledvn atltvlenyi pgffqgatdv yydevnaftr inrnffkppv seevkdmvrs
      361 nftreiefyq fckqrlhmql ralkltkktp lsgsnsl
//
LOCUS       XP_076546178             397 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 2-O-sulfotransferase pipe [Osmia lignaria
            lignaria].
ACCESSION   XP_076546178
VERSION     XP_076546178.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690063.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="heparan sulfate 2-O-sulfotransferase pipe"
                     /calculated_mol_wt=46159
     Region          109..371
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; cl22970"
                     /db_xref="CDD:451469"
     CDS             1..397
                     /gene="pip"
                     /coded_by="XM_076690063.1:1551..2744"
                     /db_xref="GeneID:117602248"
ORIGIN      
        1 mlndnvwkhr rvpvpkrtie lvalvaisst lflflhtrel hsrlrkmevr lqpgedeils
       61 anqlssegvq tdsspsgivh ypsghrpfav kykelevldi dalnntkrag rkvlffnrvp
      121 kvgsqtfmel lrrlsmrngf sfnrdrvqrv etirlapieq lqlarmvsgy aepsvyikhv
      181 cftnftefnl pqpiyinivr dpverviswy yyvrapwyyv erkqifpdlp lpdpnwlkkd
      241 fescvlkadr ecrylegeih egigdhrrqt lffcghsekc tpfntvgale rakmavekhy
      301 avvgvledvn atltvlenyi pgffqgatdv yydevnaftr inrnffkppv seevkdmvrs
      361 nftreiefyq fckqrlhmql ralkltkktp lsgsnsl
//
LOCUS       XP_034175930             397 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 2-O-sulfotransferase pipe [Osmia lignaria
            lignaria].
ACCESSION   XP_034175930
VERSION     XP_034175930.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320039.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="heparan sulfate 2-O-sulfotransferase pipe"
                     /calculated_mol_wt=46159
     Region          109..371
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; cl22970"
                     /db_xref="CDD:451469"
     CDS             1..397
                     /gene="pip"
                     /coded_by="XM_034320039.2:1332..2525"
                     /db_xref="GeneID:117602248"
ORIGIN      
        1 mlndnvwkhr rvpvpkrtie lvalvaisst lflflhtrel hsrlrkmevr lqpgedeils
       61 anqlssegvq tdsspsgivh ypsghrpfav kykelevldi dalnntkrag rkvlffnrvp
      121 kvgsqtfmel lrrlsmrngf sfnrdrvqrv etirlapieq lqlarmvsgy aepsvyikhv
      181 cftnftefnl pqpiyinivr dpverviswy yyvrapwyyv erkqifpdlp lpdpnwlkkd
      241 fescvlkadr ecrylegeih egigdhrrqt lffcghsekc tpfntvgale rakmavekhy
      301 avvgvledvn atltvlenyi pgffqgatdv yydevnaftr inrnffkppv seevkdmvrs
      361 nftreiefyq fckqrlhmql ralkltkktp lsgsnsl
//
LOCUS       XP_034175946             138 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602263 [Osmia lignaria lignaria].
ACCESSION   XP_034175946
VERSION     XP_034175946.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320055.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175946.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..138
                     /product="uncharacterized protein LOC117602263"
                     /calculated_mol_wt=15804
     CDS             1..138
                     /gene="LOC117602263"
                     /coded_by="XM_034320055.2:375..791"
                     /db_xref="GeneID:117602263"
ORIGIN      
        1 mnalqnqnth elircrynns nnnyrlmeyi nldnrtndpl sepevtmsln essaqlpvhp
       61 leeleedmfd deadamiqdi steseeqvvp ssqisniyky qeeltisdde vdegifedep
      121 tsnlvlpddn nrhdsqpp
//
LOCUS       XP_034175955             138 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602263 [Osmia lignaria lignaria].
ACCESSION   XP_034175955
VERSION     XP_034175955.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320064.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175955.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..138
                     /product="uncharacterized protein LOC117602263"
                     /calculated_mol_wt=15804
     CDS             1..138
                     /gene="LOC117602263"
                     /coded_by="XM_034320064.2:229..645"
                     /db_xref="GeneID:117602263"
ORIGIN      
        1 mnalqnqnth elircrynns nnnyrlmeyi nldnrtndpl sepevtmsln essaqlpvhp
       61 leeleedmfd deadamiqdi steseeqvvp ssqisniyky qeeltisdde vdegifedep
      121 tsnlvlpddn nrhdsqpp
//
LOCUS       XP_034173900              93 aa            linear   INV 03-SEP-2025
DEFINITION  small ubiquitin like modifier [Osmia lignaria lignaria].
ACCESSION   XP_034173900
VERSION     XP_034173900.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318009.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..93
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..93
                     /product="small ubiquitin like modifier"
                     /calculated_mol_wt=10325
     Region          14..85
                     /region_name="Ubl_SUMO2_3_4"
                     /note="ubiquitin-like (Ubl) domain found in small
                     ubiquitin-related modifier SUMO-2, SUMO-3, SUMO-4, and
                     similar proteins; cd16115"
                     /db_xref="CDD:340532"
     Site            order(14,16,25..36,38..39,43..44,46..48,53,65..67,69)
                     /site_type="other"
                     /note="SUMO2 dimer-RNF4 SIM2-3 interaction site
                     [polypeptide binding]"
                     /db_xref="CDD:340532"
     Site            order(14,23..32,39,46..48)
                     /site_type="other"
                     /note="SUMO2-TDG interaction site [polypeptide binding]"
                     /db_xref="CDD:340532"
     Site            order(14,27..32,34..36,39..40,43..44,47)
                     /site_type="other"
                     /note="SUMO3-MCAF1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340532"
     Site            order(16,20..32,35,39..40,43,47..48,50..51,53..55,58,60,
                     62,84..85)
                     /site_type="other"
                     /note="SUMO2-Rangap1-UBC9-ZNF451 complex [polypeptide
                     binding]"
                     /db_xref="CDD:340532"
     Site            order(22..30,32,39,47,56,85)
                     /site_type="other"
                     /note="SUMO2-Rangap1-UBC9-RanBP2 complex [polypeptide
                     binding]"
                     /db_xref="CDD:340532"
     Site            order(22,53,56,58,60..63,65,68,79,82,84..85)
                     /site_type="other"
                     /note="SUMO2-SENP1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340532"
     Site            order(53,56,58..61,63,65,68,73,79,82,84..85)
                     /site_type="other"
                     /note="SUMO2-SENP2 [polypeptide binding]"
                     /db_xref="CDD:340532"
     Site            order(53,56,58,60..61,63,65,68,79,82,84..85)
                     /site_type="other"
                     /note="SUMO2-SENP2-Rangap1 complex [polypeptide binding]"
                     /db_xref="CDD:340532"
     CDS             1..93
                     /gene="Sumo"
                     /coded_by="XM_034318009.2:208..489"
                     /db_xref="GeneID:117601354"
ORIGIN      
        1 msdekketkt esehinlkvl gqdsavvqfk ikkhtplrkl mnaycdrvgl aiaavrfrfd
       61 gqpineldtp ttlemeegdt ievyqqqtgg glc
//
LOCUS       XP_034173545             336 aa            linear   INV 03-SEP-2025
DEFINITION  organic solute transporter subunit alpha [Osmia lignaria lignaria].
ACCESSION   XP_034173545
VERSION     XP_034173545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317654.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="organic solute transporter subunit alpha"
                     /calculated_mol_wt=37496
     Region          75..294
                     /region_name="Solute_trans_a"
                     /note="Organic solute transporter Ostalpha; pfam03619"
                     /db_xref="CDD:460991"
     CDS             1..336
                     /gene="LOC117601194"
                     /coded_by="XM_034317654.2:177..1187"
                     /db_xref="GeneID:117601194"
ORIGIN      
        1 meieienlvn sfrnlscnhr yvpsateqie slgafgiglv sfgavlstlt lyfavdacyn
       61 viqqkearfy ktnaimillv ypvasvcslt aigiprtqll sealtqiflt ialyrlylll
      121 vyvgfkivtn spplmlkvgp cccwpclpfp nlqmtdtnls wlrlvvlqlp iiqgliycif
      181 lfmsieepsl itqysvcfqp ltvasilvgi ygltitmksl hevapeaklh rntavsqmvl
      241 lfsklqafiv kslprtglfp cnppitpeiy anvtqnalml iemlllcyaa ryvyyvdvdk
      301 eeepseatdr skdktteqvn ptnnneankq istfta
//
LOCUS       XP_034173926             444 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin delta chain [Osmia lignaria lignaria].
ACCESSION   XP_034173926
VERSION     XP_034173926.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318035.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..444
                     /product="tubulin delta chain"
                     /calculated_mol_wt=50207
     Region          2..437
                     /region_name="delta_zeta_tubulin-like"
                     /note="The delta- and zeta-tubulin families; cd02189"
                     /db_xref="CDD:276958"
     Site            order(7..9,12..13,66..68,71,98..100,139..145,170,172..173,
                     180..181,204,208,222,225..226,229)
                     /site_type="other"
                     /note="putative nucleotide binding site [chemical
                     binding]"
                     /db_xref="CDD:276958"
     CDS             1..444
                     /gene="LOC117601363"
                     /coded_by="XM_034318035.2:527..1861"
                     /db_xref="GeneID:117601363"
ORIGIN      
        1 mltiqfgqcg nqlghtlfsk issdlectnt gisynanyqy sedtfnkwfn giskhnkwlp
       61 ravlidteek viskiykdrs tpwtyskesi icqagggcan nwafgylrkg pelsslvldc
      121 vrqildvtdy fdgfllllss aggtgsgigs fiaqtlreey rtkpiittti lpfsfgevct
      181 qkyntlltla klynqcdini lieneqvhni stnllkksnt tlqdinaiis ekllaifqpl
      241 ndtnmnslls qiashpsykl atikstphvs tvsaeyepvc nwnsyvhhlk qtlripnlnm
      301 qltnaelkap ttsfsssthy vyacsvsnil itrglttdnd sivtsdfkek nlyanwntid
      361 wfshlhqnrk ilnhekflal ltnnsqisnp ldllldktwn syvhaaflhq ykqygleedd
      421 flqafallen vvkeykelva hmnk
//
LOCUS       XP_034173579            1840 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601202 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173579
VERSION     XP_034173579.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317688.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173579.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1840
                     /product="uncharacterized protein LOC117601202 isoform X1"
                     /calculated_mol_wt=205477
     Region          174..>312
                     /region_name="COG5644"
                     /note="U3 small nucleolar RNA-associated protein 14
                     [Function unknown]"
                     /db_xref="CDD:227931"
     Region          1337..1480
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1520..1658
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1840
                     /gene="LOC117601202"
                     /coded_by="XM_034317688.2:656..6178"
                     /db_xref="GeneID:117601202"
ORIGIN      
        1 mayvlwrvfm kkyskvrlgi rnlpvipaer rvpekeetws lapdqdsctf fdeipdsksk
       61 aaqdvdggqs rivgesvpge snenpesgtl prsqetrksk tkkvksylrk ckgalskgde
      121 giaekkrqeh ctswyleesr vhqedtdvlp ekytesryqr vveseevpvv pvdeddstlr
      181 vdsdpstnln kddvldetvd edlsrltddd rrnqlkrsrt slyedakesm ndqctveeis
      241 asnkmegcer ndavsletlt tedtnlnkcd sndtliaevt etvivesnss savdgeqeke
      301 kesrdegids eilpalslpg drrdvaslvr nllgpiygdg vinllirqar dilvcayhgn
      361 lenflrtyls paasllaevk tvasetenes vvpegwpltl grrglvlqlg eleasalrlg
      421 ecylcirsaa avattlgsge aaagetvern tveiavvwrt tsmrapgflg wdreeefmdw
      481 revsrkrqgn ssgtssslvg pqsssssksm mrprrrsree trrprepgfv lehrreetrs
      541 idrleyknsn eactvdgeve tghavmksrs tssmdtwkag ldgdlngmpi rpgpnnsssl
      601 sgddgrvkvk rcakvvsstt mekwkettrc dtrsktlvpe dlktplrldq avlgpkdgkg
      661 fgvaedrlqn vrhvdevgdd elpafignll vsverkierv slddltfpcp acrnidtddp
      721 llgcrcagde cgcgagedtc dcsssmktrt eqsakcfeia gikhidsdde eevrmgfgtk
      781 krvddvvapr tihdipehvl tcgltlpgyt dangrsvlef edepsrretl svkevssfll
      841 ylarlpstes tkngftllvr gnapdclefl dkvlslvqgr vnvsqallsp sfvaehstes
      901 qlplshvkti amdehalrer vprrenpdqa qwiafykeyd slmtewqaag rrlalemsel
      961 kecsslsgal tplgrlltdp tlrrtsrdae eaissleera spllhlehir lavkraqrlv
     1021 eevgnaatrl essfesrrat irnlavlrsi edqahevlsw lckkgedils khaqhsatnl
     1081 taarlherdf ekfyftsmrh ldkgsdlaea asasglrela rslkqhlrgf gsrledrrey
     1141 ledtsrccll qdrayewaqe arlagergaq qflmarpplp pehftemmal aeklgneili
     1201 eqcriarnkc aevlemtrtt sapgsparrr sfaasesaas wedsltkrts wddsdssasy
     1261 acpmesvgst gsggrpvldn iaelresaeq lldcspprtp prspaprrlv kppvnshlhr
     1321 aasiapgmka kktillimre miqterdyvk sleyiienyi pelvredipq alrgqrnvif
     1381 gnvekiyefh sqhflreleq ceqspmnvgq cflrhekkfy lyalynknkp nsdslmaeyg
     1441 tvffkqkqle lgdkmdlasy llkpvqrmgk yalllqqlvk agtdlseqts gkeekeeked
     1501 gmkpmvegea dlraaeqmvr fqlrhgndll amdslrdcdv nvkeqgrllr qneflvwqgk
     1561 gkkclrqvfl fedlilfska rrfpdrknld iyiykhsikt tdigltavia dsptkfeiwf
     1621 rkrkpgdtyt lqcasedikk awteelsnll wkqalrnrev rlaemssmgi gnkpcldirp
     1681 sadqindrsi svaqlsktpr frnsiavsms edsgrcsrrp hsvisvssss ssggssgppt
     1741 tlnlgldtsp rphhrsttln sqcsvesgii adisivsddg gdgtershwn stlesptshl
     1801 pttstplqsp tsatatgtvt satssesnlt pydqdmsinl
//
LOCUS       XP_034173588            1829 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601202 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173588
VERSION     XP_034173588.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317697.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173588.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1829
                     /product="uncharacterized protein LOC117601202 isoform X2"
                     /calculated_mol_wt=204252
     Region          163..>301
                     /region_name="COG5644"
                     /note="U3 small nucleolar RNA-associated protein 14
                     [Function unknown]"
                     /db_xref="CDD:227931"
     Region          1326..1469
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1509..1647
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1829
                     /gene="LOC117601202"
                     /coded_by="XM_034317697.2:1159..6648"
                     /db_xref="GeneID:117601202"
ORIGIN      
        1 mshfdwkllr rkkvipaerr vpekeetwsl apdqdsctff deipdskska aqdvdggqsr
       61 ivgesvpges nenpesgtlp rsqetrkskt kkvksylrkc kgalskgdeg iaekkrqehc
      121 tswyleesrv hqedtdvlpe kytesryqrv veseevpvvp vdeddstlrv dsdpstnlnk
      181 ddvldetvde dlsrltdddr rnqlkrsrts lyedakesmn dqctveeisa snkmegcern
      241 davsletltt edtnlnkcds ndtliaevte tvivesnsss avdgeqekek esrdegidse
      301 ilpalslpgd rrdvaslvrn llgpiygdgv inllirqard ilvcayhgnl enflrtylsp
      361 aasllaevkt vasetenesv vpegwpltlg rrglvlqlge leasalrlge cylcirsaaa
      421 vattlgsgea aagetvernt veiavvwrtt smrapgflgw dreeefmdwr evsrkrqgns
      481 sgtssslvgp qsssssksmm rprrrsreet rrprepgfvl ehrreetrsi drleyknsne
      541 actvdgevet ghavmksrst ssmdtwkagl dgdlngmpir pgpnnsssls gddgrvkvkr
      601 cakvvssttm ekwkettrcd trsktlvped lktplrldqa vlgpkdgkgf gvaedrlqnv
      661 rhvdevgdde lpafignllv sverkiervs lddltfpcpa crnidtddpl lgcrcagdec
      721 gcgagedtcd csssmktrte qsakcfeiag ikhidsddee evrmgfgtkk rvddvvaprt
      781 ihdipehvlt cgltlpgytd angrsvlefe depsrretls vkevssflly larlpstest
      841 kngftllvrg napdclefld kvlslvqgrv nvsqallsps fvaehstesq lplshvktia
      901 mdehalrerv prrenpdqaq wiafykeyds lmtewqaagr rlalemselk ecsslsgalt
      961 plgrlltdpt lrrtsrdaee aissleeras pllhlehirl avkraqrlve evgnaatrle
     1021 ssfesrrati rnlavlrsie dqahevlswl ckkgedilsk haqhsatnlt aarlherdfe
     1081 kfyftsmrhl dkgsdlaeaa sasglrelar slkqhlrgfg srledrreyl edtsrccllq
     1141 drayewaqea rlagergaqq flmarpplpp ehftemmala eklgneilie qcriarnkca
     1201 evlemtrtts apgsparrrs faasesaasw edsltkrtsw ddsdssasya cpmesvgstg
     1261 sggrpvldni aelresaeql ldcspprtpp rspaprrlvk ppvnshlhra asiapgmkak
     1321 ktillimrem iqterdyvks leyiienyip elvredipqa lrgqrnvifg nvekiyefhs
     1381 qhflreleqc eqspmnvgqc flrhekkfyl yalynknkpn sdslmaeygt vffkqkqlel
     1441 gdkmdlasyl lkpvqrmgky alllqqlvka gtdlseqtsg keekeekedg mkpmvegead
     1501 lraaeqmvrf qlrhgndlla mdslrdcdvn vkeqgrllrq neflvwqgkg kkclrqvflf
     1561 edlilfskar rfpdrknldi yiykhsiktt digltaviad sptkfeiwfr krkpgdtytl
     1621 qcasedikka wteelsnllw kqalrnrevr laemssmgig nkpcldirps adqindrsis
     1681 vaqlsktprf rnsiavsmse dsgrcsrrph svisvsssss sggssgpptt lnlgldtspr
     1741 phhrsttlns qcsvesgiia disivsddgg dgtershwns tlesptshlp ttstplqspt
     1801 satatgtvts atssesnltp ydqdmsinl
//
LOCUS       XP_034173597            1798 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601202 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173597
VERSION     XP_034173597.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317706.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173597.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1798
                     /product="uncharacterized protein LOC117601202 isoform X3"
                     /calculated_mol_wt=200584
     Region          174..>312
                     /region_name="COG5644"
                     /note="U3 small nucleolar RNA-associated protein 14
                     [Function unknown]"
                     /db_xref="CDD:227931"
     Region          1295..1438
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1478..1616
                     /region_name="PH_puratrophin-1"
                     /note="Puratrophin-1 pleckstrin homology (PH) domain;
                     cd13242"
                     /db_xref="CDD:270062"
     CDS             1..1798
                     /gene="LOC117601202"
                     /coded_by="XM_034317706.2:694..6090"
                     /db_xref="GeneID:117601202"
ORIGIN      
        1 mayvlwrvfm kkyskvrlgi rnlpvipaer rvpekeetws lapdqdsctf fdeipdsksk
       61 aaqdvdggqs rivgesvpge snenpesgtl prsqetrksk tkkvksylrk ckgalskgde
      121 giaekkrqeh ctswyleesr vhqedtdvlp ekytesryqr vveseevpvv pvdeddstlr
      181 vdsdpstnln kddvldetvd edlsrltddd rrnqlkrsrt slyedakesm ndqctveeis
      241 asnkmegcer ndavsletlt tedtnlnkcd sndtliaevt etvivesnss savdgeqeke
      301 kesrdegids eilpalslpg drrdvaslvr nllgpiygdg vinllirqar dilvcayhgn
      361 lenflrtyls paasllaevk tvasetenes vvpegwpltl grrglvlqlg eleasalrlg
      421 ecylcirsaa avattlgsge aaagetvern tveiavvwrt tsmrapgflg wdreeefmdw
      481 revsrkrqgn ssgtssslvg pqsssssksm mrprrrsree trrprepgfv lehrreetrs
      541 idrleyknsn eactvdgeve tghavmksrs tssmdtwkag ldgdlngmpi rpgpnnsssl
      601 sgddgrvkvk rcakvvsstt mekwkettrc dtrsktlvpe dlktplrldq avlgpkdgkg
      661 fgvaedrlqn vrhvdevgdd elpafignll vsverkierv slddltfpcp acrnidtddp
      721 llgcrcagde cgcgagedtc dcsssmktrt eqsakcfeia gikhidsdde eevrmgfgtk
      781 krvddvvapr tihdipehvl tcgltlpgyt dangrsvlef edepsrretl svkevssfll
      841 ylarlpstes tkngftllvr gnapdclefl dkvlslvqgr vnvsqallsp sfvaehstes
      901 qlplshvkti amdehalrer vprrenpdqa qwiafykeyd slmtewqaag rrlalemsel
      961 kecsslsgal tplgrlltdp tlrrtsrdae eaissleera spllhlehir lavkraqrlv
     1021 eevgnaatrl essfesrrat irnlavlrsi edqaherhld kgsdlaeaas asglrelars
     1081 lkqhlrgfgs rledrreyle dtsrccllqd rayewaqear lagergaqqf lmarpplppe
     1141 hftemmalae klgneilieq criarnkcae vlemtrttsa pgsparrrsf aasesaaswe
     1201 dsltkrtswd dsdssasyac pmesvgstgs ggrpvldnia elresaeqll dcspprtppr
     1261 spaprrlvkp pvnshlhraa siapgmkakk tillimremi qterdyvksl eyiienyipe
     1321 lvredipqal rgqrnvifgn vekiyefhsq hflreleqce qspmnvgqcf lrhekkfyly
     1381 alynknkpns dslmaeygtv ffkqkqlelg dkmdlasyll kpvqrmgkya lllqqlvkag
     1441 tdlseqtsgk eekeekedgm kpmvegeadl raaeqmvrfq lrhgndllam dslrdcdvnv
     1501 keqgrllrqn eflvwqgkgk kclrqvflfe dlilfskarr fpdrknldiy iykhsikttd
     1561 igltaviads ptkfeiwfrk rkpgdtytlq casedikkaw teelsnllwk qalrnrevrl
     1621 aemssmgign kpcldirpsa dqindrsisv aqlsktprfr nsiavsmsed sgrcsrrphs
     1681 visvssssss ggssgppttl nlgldtsprp hhrsttlnsq csvesgiiad isivsddggd
     1741 gtershwnst lesptshlpt tstplqspts atatgtvtsa tssesnltpy dqdmsinl
//
LOCUS       XP_034173606             717 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601219 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173606
VERSION     XP_034173606.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317715.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..717
                     /product="uncharacterized protein LOC117601219 isoform X1"
                     /calculated_mol_wt=81807
     Region          <229..502
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          230..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(235,237,239,241..242,245..246,249,263,265,269..270,
                     273..274,277,291,293,295,297..298,301..302,308)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          258..278
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          286..309
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          315..335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(320,322,324,326..327,330..331,334,348,350,354..355,
                     358..359,362,376,378,380,382..383,386..387,390)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          343..363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          371..391
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          401..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          430..450
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          458..478
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          486..507
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          521..543
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          523..543
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..717
                     /gene="LOC117601219"
                     /coded_by="XM_034317715.2:420..2573"
                     /db_xref="GeneID:117601219"
ORIGIN      
        1 mtsnvatikl eegqesvtei qtyletfnke ieggqgeqlq hvqlqqvegl sggeeggtyf
       61 vdqsgqyyyq anndetpvmt qvqiqeveet dvqnegehpq eeqyqeieel envdgeedgq
      121 vanngnnqvv insgdayqtv tivpsdtnpg evsyvlivqq pdseekeirp veqegtegee
      181 gegeqdltvy dfednednea pveseveddk tkiikflpkk sqtvtqahmc nycnytspkr
      241 yllsrhmksh seerphkcsv cergfktlas lqnhvnthtg tkphhckfcd safttsgelv
      301 rhvryrhthe kphkchecdy asvelsklkr hirchtgerp yqcphctyas pdtfklkrhl
      361 rihtgekpye cdfcqarftq snslkahkli hnvgdkpvfq celcpttcgr ktdlrihvqk
      421 lhtsdkplkc krcgktfpdr ysyklhskth egekcykcdl cpyasisarh leshmlihtd
      481 qkpyqcdhcf qsfrqkqllk rhcnlyhnps yvppppqekt hqcpecerpf rhkgnlirhm
      541 avhdpesslq ekqqalkmgr qkkiqiidgq rvevmtgdla sklkgyeeee ededdmmave
      601 gsdgqqyvvl eviqladnqg adqqmavvas edgdlvmqdp lsqesgivtg tgeegddeee
      661 eteiklkidp epsiksqntq ndsklqkeme tcfgfdeeee eedeeangni niletis
//
LOCUS       XP_034173615             717 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601219 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173615
VERSION     XP_034173615.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317724.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..717
                     /product="uncharacterized protein LOC117601219 isoform X1"
                     /calculated_mol_wt=81807
     Region          <229..502
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          230..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(235,237,239,241..242,245..246,249,263,265,269..270,
                     273..274,277,291,293,295,297..298,301..302,308)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          258..278
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          286..309
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          315..335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(320,322,324,326..327,330..331,334,348,350,354..355,
                     358..359,362,376,378,380,382..383,386..387,390)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          343..363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          371..391
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          401..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          430..450
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          458..478
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          486..507
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          521..543
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          523..543
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..717
                     /gene="LOC117601219"
                     /coded_by="XM_034317724.2:286..2439"
                     /db_xref="GeneID:117601219"
ORIGIN      
        1 mtsnvatikl eegqesvtei qtyletfnke ieggqgeqlq hvqlqqvegl sggeeggtyf
       61 vdqsgqyyyq anndetpvmt qvqiqeveet dvqnegehpq eeqyqeieel envdgeedgq
      121 vanngnnqvv insgdayqtv tivpsdtnpg evsyvlivqq pdseekeirp veqegtegee
      181 gegeqdltvy dfednednea pveseveddk tkiikflpkk sqtvtqahmc nycnytspkr
      241 yllsrhmksh seerphkcsv cergfktlas lqnhvnthtg tkphhckfcd safttsgelv
      301 rhvryrhthe kphkchecdy asvelsklkr hirchtgerp yqcphctyas pdtfklkrhl
      361 rihtgekpye cdfcqarftq snslkahkli hnvgdkpvfq celcpttcgr ktdlrihvqk
      421 lhtsdkplkc krcgktfpdr ysyklhskth egekcykcdl cpyasisarh leshmlihtd
      481 qkpyqcdhcf qsfrqkqllk rhcnlyhnps yvppppqekt hqcpecerpf rhkgnlirhm
      541 avhdpesslq ekqqalkmgr qkkiqiidgq rvevmtgdla sklkgyeeee ededdmmave
      601 gsdgqqyvvl eviqladnqg adqqmavvas edgdlvmqdp lsqesgivtg tgeegddeee
      661 eteiklkidp epsiksqntq ndsklqkeme tcfgfdeeee eedeeangni niletis
//
LOCUS       XP_034173626             717 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601219 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173626
VERSION     XP_034173626.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317735.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..717
                     /product="uncharacterized protein LOC117601219 isoform X1"
                     /calculated_mol_wt=81807
     Region          <229..502
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          230..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(235,237,239,241..242,245..246,249,263,265,269..270,
                     273..274,277,291,293,295,297..298,301..302,308)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          258..278
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          286..309
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          315..335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(320,322,324,326..327,330..331,334,348,350,354..355,
                     358..359,362,376,378,380,382..383,386..387,390)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          343..363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          371..391
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          401..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          430..450
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          458..478
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          486..507
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          521..543
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          523..543
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..717
                     /gene="LOC117601219"
                     /coded_by="XM_034317735.2:137..2290"
                     /db_xref="GeneID:117601219"
ORIGIN      
        1 mtsnvatikl eegqesvtei qtyletfnke ieggqgeqlq hvqlqqvegl sggeeggtyf
       61 vdqsgqyyyq anndetpvmt qvqiqeveet dvqnegehpq eeqyqeieel envdgeedgq
      121 vanngnnqvv insgdayqtv tivpsdtnpg evsyvlivqq pdseekeirp veqegtegee
      181 gegeqdltvy dfednednea pveseveddk tkiikflpkk sqtvtqahmc nycnytspkr
      241 yllsrhmksh seerphkcsv cergfktlas lqnhvnthtg tkphhckfcd safttsgelv
      301 rhvryrhthe kphkchecdy asvelsklkr hirchtgerp yqcphctyas pdtfklkrhl
      361 rihtgekpye cdfcqarftq snslkahkli hnvgdkpvfq celcpttcgr ktdlrihvqk
      421 lhtsdkplkc krcgktfpdr ysyklhskth egekcykcdl cpyasisarh leshmlihtd
      481 qkpyqcdhcf qsfrqkqllk rhcnlyhnps yvppppqekt hqcpecerpf rhkgnlirhm
      541 avhdpesslq ekqqalkmgr qkkiqiidgq rvevmtgdla sklkgyeeee ededdmmave
      601 gsdgqqyvvl eviqladnqg adqqmavvas edgdlvmqdp lsqesgivtg tgeegddeee
      661 eteiklkidp epsiksqntq ndsklqkeme tcfgfdeeee eedeeangni niletis
//
LOCUS       XP_034173636             717 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601219 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173636
VERSION     XP_034173636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..717
                     /product="uncharacterized protein LOC117601219 isoform X1"
                     /calculated_mol_wt=81807
     Region          <229..502
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          230..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(235,237,239,241..242,245..246,249,263,265,269..270,
                     273..274,277,291,293,295,297..298,301..302,308)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          258..278
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          286..309
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          315..335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(320,322,324,326..327,330..331,334,348,350,354..355,
                     358..359,362,376,378,380,382..383,386..387,390)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          343..363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          371..391
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          401..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          430..450
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          458..478
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          486..507
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          521..543
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          523..543
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..717
                     /gene="LOC117601219"
                     /coded_by="XM_034317745.2:580..2733"
                     /db_xref="GeneID:117601219"
ORIGIN      
        1 mtsnvatikl eegqesvtei qtyletfnke ieggqgeqlq hvqlqqvegl sggeeggtyf
       61 vdqsgqyyyq anndetpvmt qvqiqeveet dvqnegehpq eeqyqeieel envdgeedgq
      121 vanngnnqvv insgdayqtv tivpsdtnpg evsyvlivqq pdseekeirp veqegtegee
      181 gegeqdltvy dfednednea pveseveddk tkiikflpkk sqtvtqahmc nycnytspkr
      241 yllsrhmksh seerphkcsv cergfktlas lqnhvnthtg tkphhckfcd safttsgelv
      301 rhvryrhthe kphkchecdy asvelsklkr hirchtgerp yqcphctyas pdtfklkrhl
      361 rihtgekpye cdfcqarftq snslkahkli hnvgdkpvfq celcpttcgr ktdlrihvqk
      421 lhtsdkplkc krcgktfpdr ysyklhskth egekcykcdl cpyasisarh leshmlihtd
      481 qkpyqcdhcf qsfrqkqllk rhcnlyhnps yvppppqekt hqcpecerpf rhkgnlirhm
      541 avhdpesslq ekqqalkmgr qkkiqiidgq rvevmtgdla sklkgyeeee ededdmmave
      601 gsdgqqyvvl eviqladnqg adqqmavvas edgdlvmqdp lsqesgivtg tgeegddeee
      661 eteiklkidp epsiksqntq ndsklqkeme tcfgfdeeee eedeeangni niletis
//
LOCUS       XP_034173643             709 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601219 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173643
VERSION     XP_034173643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317752.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..709
                     /product="uncharacterized protein LOC117601219 isoform X2"
                     /calculated_mol_wt=80994
     Region          <229..502
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          230..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(235,237,239,241..242,245..246,249,263,265,269..270,
                     273..274,277,291,293,295,297..298,301..302,308)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          258..278
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          286..309
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          315..335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(320,322,324,326..327,330..331,334,348,350,354..355,
                     358..359,362,376,378,380,382..383,386..387,390)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          343..363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          371..391
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          401..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          430..450
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          458..478
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          486..507
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          521..543
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          523..543
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..709
                     /gene="LOC117601219"
                     /coded_by="XM_034317752.2:407..2536"
                     /db_xref="GeneID:117601219"
ORIGIN      
        1 mtsnvatikl eegqesvtei qtyletfnke ieggqgeqlq hvqlqqvegl sggeeggtyf
       61 vdqsgqyyyq anndetpvmt qvqiqeveet dvqnegehpq eeqyqeieel envdgeedgq
      121 vanngnnqvv insgdayqtv tivpsdtnpg evsyvlivqq pdseekeirp veqegtegee
      181 gegeqdltvy dfednednea pveseveddk tkiikflpkk sqtvtqahmc nycnytspkr
      241 yllsrhmksh seerphkcsv cergfktlas lqnhvnthtg tkphhckfcd safttsgelv
      301 rhvryrhthe kphkchecdy asvelsklkr hirchtgerp yqcphctyas pdtfklkrhl
      361 rihtgekpye cdfcqarftq snslkahkli hnvgdkpvfq celcpttcgr ktdlrihvqk
      421 lhtsdkplkc krcgktfpdr ysyklhskth egekcykcdl cpyasisarh leshmlihtd
      481 qkpyqcdhcf qsfrqkqllk rhcnlyhnps yvppppqekt hqcpecerpf rhkgnlirhm
      541 avhdpesslq ekqqalkmgr qkkiqiidgq rvevmtgyee eeededdmma vegsdgqqyv
      601 vleviqladn qgadqqmavv asedgdlvmq dplsqesgiv tgtgeegdde eeeteiklki
      661 dpepsiksqn tqndsklqke metcfgfdee eeeedeeang niniletis
//
LOCUS       XP_034173652             663 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601219 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173652
VERSION     XP_034173652.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317761.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..663
                     /product="uncharacterized protein LOC117601219 isoform X3"
                     /calculated_mol_wt=75359
     Region          <229..439
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          230..250
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(235,237,239,241..242,245..246,249,263,265,269..270,
                     273..274,277,291,293,295,297..298,301..302,308)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          258..278
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          270..295
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          286..309
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          315..335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(320,322,324,326..327,330..331,334,348,350,354..355,
                     358..359,362,376,378,380,382..383,386..387,390)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          328..349
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          343..363
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          356..380
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          371..391
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          401..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          430..453
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          467..489
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          469..489
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..663
                     /gene="LOC117601219"
                     /coded_by="XM_034317761.2:422..2413"
                     /db_xref="GeneID:117601219"
ORIGIN      
        1 mtsnvatikl eegqesvtei qtyletfnke ieggqgeqlq hvqlqqvegl sggeeggtyf
       61 vdqsgqyyyq anndetpvmt qvqiqeveet dvqnegehpq eeqyqeieel envdgeedgq
      121 vanngnnqvv insgdayqtv tivpsdtnpg evsyvlivqq pdseekeirp veqegtegee
      181 gegeqdltvy dfednednea pveseveddk tkiikflpkk sqtvtqahmc nycnytspkr
      241 yllsrhmksh seerphkcsv cergfktlas lqnhvnthtg tkphhckfcd safttsgelv
      301 rhvryrhthe kphkchecdy asvelsklkr hirchtgerp yqcphctyas pdtfklkrhl
      361 rihtgekpye cdfcqarftq snslkahkli hnvgdkpvfq celcpttcgr ktdlrihvqk
      421 lhtsdkplkc krcgktfpdr qkqllkrhcn lyhnpsyvpp ppqekthqcp ecerpfrhkg
      481 nlirhmavhd pesslqekqq alkmgrqkki qiidgqrvev mtgdlasklk gyeeeeeded
      541 dmmavegsdg qqyvvleviq ladnqgadqq mavvasedgd lvmqdplsqe sgivtgtgee
      601 gddeeeetei klkidpepsi ksqntqndsk lqkemetcfg fdeeeeeede eangninile
      661 tis
//
LOCUS       XP_034173664             301 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin 17 [Osmia lignaria lignaria].
ACCESSION   XP_034173664
VERSION     XP_034173664.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173664.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..301
                     /product="syntaxin 17"
                     /calculated_mol_wt=33919
     Region          153..209
                     /region_name="SNARE_syntaxin17"
                     /note="SNARE motif of syntaxin 17; cd15846"
                     /db_xref="CDD:277199"
     Site            order(154..155,157..162,164..166,168..169,171..173,
                     175..176,178..180,182..183,185..190,192..197,199..204,
                     206..208)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277199"
     Site            182
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277199"
     CDS             1..301
                     /gene="Syx17"
                     /coded_by="XM_034317773.2:268..1173"
                     /db_xref="GeneID:117601243"
ORIGIN      
        1 mstssgnial kqpirrleiq innfnvaiph hvgllkrhkc niqkyqaehd wekmykehmn
       61 vsriikqlke llyqmdtlra qvldsdinef dkltgnarss imnavneyle lqlnlpispp
      121 dlpkskdqak ehpldnryvq lqeeqqelqr qqaclhawds lqgdihhlhq lfvdfnkivd
      181 dqkelvntae ddieetnvnv kegekflvka vrykaamypi agavigtcig gpvgliaglk
      241 vggltalgcg ilgftgasll kkkeiemqks rstmeqslte qksvqkstsl penlnetkke
      301 l
//
LOCUS       XP_034173678             256 aa            linear   INV 03-SEP-2025
DEFINITION  farnesol dehydrogenase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034173678
VERSION     XP_034173678.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317787.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173678.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="farnesol dehydrogenase isoform X1"
                     /calculated_mol_wt=28274
     Region          1..253
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(13,15..16,18,38..40,92..94,143..145,168,172,
                     200..203)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187601"
     Site            order(116,145,168,172)
                     /site_type="active"
                     /db_xref="CDD:187601"
     CDS             1..256
                     /gene="LOC117601278"
                     /coded_by="XM_034317787.2:924..1694"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 merwagknav vtgaasgige aitaallknk gnvvaldirf eelllaarkw eqikhrgnir
       61 cmycdvsept nleevfstie rewkgvdimv nnagmvdytr viesdwetfq rllnfnvlat
      121 smcinwavrs mrqrnveghi fninsvlghe ipsgslseid gcngwnlypt ckhgtvalth
      181 tvrrelaaik apiritsisp givntgiakh vsqltdvlkr ipalrpedva daliyalgsr
      241 pevqiselti qrtgep
//
LOCUS       XP_076546179             253 aa            linear   INV 03-SEP-2025
DEFINITION  farnesol dehydrogenase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546179
VERSION     XP_076546179.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690064.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="farnesol dehydrogenase isoform X2"
                     /calculated_mol_wt=28102
     Region          1..245
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(13,15..16,18,38..40,92..94,143..145,168,172,
                     200..203)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187601"
     Site            order(116,145,168,172)
                     /site_type="active"
                     /db_xref="CDD:187601"
     CDS             1..253
                     /gene="LOC117601278"
                     /coded_by="XM_076690064.1:411..1172"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 merwagknav vtgaasgige aitaallknk gnvvaldirf eelllaarkw eqikhrgnir
       61 cmycdvsept nleevfstie rewkgvdimv nnagmvdytr viesdwetfq rllnfnvlat
      121 smcinwavrs mrqrnveghi fninsvlghe ipsgslseid gcngwnlypt ckhgtvalth
      181 tvrrelaaik apiritsisp givntgiakh vsqltdvlkr ipalrpedva daliyalgsr
      241 pevqivnewq rid
//
LOCUS       XP_034173756             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173756
VERSION     XP_034173756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_034317865.2:866..1552"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_076546180             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546180
VERSION     XP_076546180.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690065.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_076690065.1:9263..9949"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_034173766             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173766
VERSION     XP_034173766.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317875.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_034317875.2:426..1112"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_076546181             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546181
VERSION     XP_076546181.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690066.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_076690066.1:624..1310"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_034173775             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173775
VERSION     XP_034173775.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317884.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_034317884.2:1067..1753"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_034173784             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173784
VERSION     XP_034173784.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_034317893.2:951..1637"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_076546182             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546182
VERSION     XP_076546182.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690067.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_076690067.1:261..947"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_076546183             228 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601278 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546183
VERSION     XP_076546183.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690068.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="uncharacterized protein LOC117601278 isoform X3"
                     /calculated_mol_wt=25609
     CDS             1..228
                     /gene="LOC117601278"
                     /coded_by="XM_076690068.1:490..1176"
                     /db_xref="GeneID:117601278"
ORIGIN      
        1 mlpsskllqe epatestaas tsrdpssvpi tlpntpvdha tdddpppysd ivppnhigwt
       61 ydfsaipysa ctstpcrqte vplasfqtsg scfhpegtds qhtsipmplm scrlfmfgsh
      121 rslfshsnlp fsdnisiskd sqgktrigcr efrswksfyp grgqrvtnlf eckvqrknat
      181 rfteifhpfq irgtrvtrsl tlhafqlphn stlwtfitdk lfycnkrv
//
LOCUS       XP_034195213             684 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611371 [Osmia lignaria lignaria].
ACCESSION   XP_034195213
VERSION     XP_034195213.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339322.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="uncharacterized protein LOC117611371"
                     /calculated_mol_wt=78506
     CDS             1..684
                     /gene="LOC117611371"
                     /coded_by="XM_034339322.2:201..2255"
                     /db_xref="GeneID:117611371"
ORIGIN      
        1 mqilktwipd eliffiiwgc lfcsvgwkll hapsnvprrr silettnsss krsskdqana
       61 rnlpcaqesq fgreersnsn fkiiirlpek vlkivlkyie kllltilinv ykniqkychv
      121 saqvesverl rnllnkssnn rinvtmnnqs nynvlneetp nkqssinekt sptnkisfhe
      181 yltrflykdi dqsqekfnnd vtseenssdv agkqrekyae nvyerknedv tgndndlpsh
      241 kfgscskaar ynvddnsars nmkinenaes llirslaemf lasilrdsev fgpeskemee
      301 neetkensdt ylfddesrse mladkleehl tfaeesdkic srteddypge qsgvvpekev
      361 nllenesvmh sskgatqfpv ekkekgeefg sppdtnslkn crdsesagfs dlspvtdhgk
      421 eeedlnalvr rmiivelkkh rkkeeivlkr ahsdtetvip rkkqnsksrt glfslkgrtf
      481 frdllelees lrrnsdness esvkvemneg mtkaeesinq genhskndvs vvtdcanvse
      541 nstdamcktn ssimlmrkih vpclkstene neqsiefeti fgapkeelks kctqtegaym
      601 vravrscplr krrvslqlqq lhrltdrepy ystivyankd fsrfiyrkas dnliyekgkg
      661 srspsyipap erfrwkylip nkvk
//
LOCUS       XP_034196179             211 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611872 [Osmia lignaria lignaria].
ACCESSION   XP_034196179
VERSION     XP_034196179.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340288.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..211
                     /product="uncharacterized protein LOC117611872"
                     /calculated_mol_wt=24678
     Region          6..68
                     /region_name="Linker_histone"
                     /note="linker histone H1 and H5 family; pfam00538"
                     /db_xref="CDD:459846"
     CDS             1..211
                     /gene="LOC117611872"
                     /coded_by="XM_034340288.1:340..975"
                     /db_xref="GeneID:117611872"
ORIGIN      
        1 mgrqvpkmsa rvvnairnlr eahgstskei mnyimsqysa peatiqrqmq aalkrgldyg
       61 ilkknnghyf lntdtdvael assmapvers rrrrsrrrks rrrsrgrrrr rgrrgrsrrr
      121 rrrsgrrraa ttrrigctrc rctkrsrnmh vlrdepveqq egptcdcene inqerqnrqs
      181 ksrerslshs rssansdrdg addrqeipdq d
//
LOCUS       XP_034195407             421 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611552 [Osmia lignaria lignaria].
ACCESSION   XP_034195407
VERSION     XP_034195407.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339516.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195407.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="uncharacterized protein LOC117611552"
                     /calculated_mol_wt=45678
     Region          <66..90
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     CDS             1..421
                     /gene="LOC117611552"
                     /coded_by="XM_034339516.2:20..1285"
                     /db_xref="GeneID:117611552"
ORIGIN      
        1 milllaclsl ligpgelgnl nngpsvgvtt ifglgalpfg faagpryvpk wkkqaceipa
       61 sqhpnshyvc deagevkclp gwtgdlcdvp icrkgcdplq gycrrpsecr cklgfygelc
      121 dkcvtlpgcq hgscnvsfec scdpgwkgmf csepicaadc lpnqgycekp gecrcrlgwq
      181 gpkckqcavl pgcvhgtcqg plecrcepgw tgllcqtpic sqgcsrehgs crrpgtcrcr
      241 vgwtgpncte cvpypgcvhg sckrpwecrc epgwsgdlcn ekltycdehq gicknnatci
      301 smtkedgnyr cicplgymgr qcqirtmvps telvpptpds teseakpqmv pvkpevness
      361 qsleekdknm vkeeeqtvep ldpivitdpp stidpaatvg kwptepptes pvterddene
      421 a
//
LOCUS       XP_034177728            3786 aa            linear   INV 03-SEP-2025
DEFINITION  laminin subunit alpha [Osmia lignaria lignaria].
ACCESSION   XP_034177728
VERSION     XP_034177728.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321837.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177728.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3786
                     /product="laminin subunit alpha"
                     /calculated_mol_wt=418999
     Region          148..394
                     /region_name="LamNT"
                     /note="Laminin N-terminal domain (domain VI); smart00136"
                     /db_xref="CDD:214532"
     Region          395..>436
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(396,398,405,418,420,429)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          454..514
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(455,457,464,489,492,501)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          525..572
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(525,527,536,543,545,554)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          570..618
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(570,572,582,589,591,600)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          615..657
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(616,618,628,635,637,646)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          662..710
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(662,664,674,681,683,692)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          708..755
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(708,710,720,726,728,737)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          753..800
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(753,755,765,771,773,782)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          798..850
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(798,800,812,821,823,832)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          853..903
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(853,855,867,874,876,885)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          906..949
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(906,908,918,925,927,936)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          952..1087
                     /region_name="CBM6-CBM35-CBM36_like"
                     /note="Carbohydrate Binding Module 6 (CBM6) and CBM35_like
                     superfamily; cd02795"
                     /db_xref="CDD:271143"
     Region          1488..1536
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1488,1490,1500,1507,1509,1518)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1533..1570
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1534,1536,1542,1549,1551,1560)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1579..1629
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1579,1581,1593,1600,1602,1611)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1627..1675
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1627,1629,1639,1646,1648,1657)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1745..1880
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1879..2192
                     /region_name="VSP"
                     /note="Giardia variant-specific surface protein;
                     pfam03302"
                     /db_xref="CDD:146106"
     Region          1914..1957
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1915,1917,1924,1931,1934,1943)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2022..2074
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(2023,2025,2037,2044,2047,2056)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2123..2167
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(2123,2125,2134,2141,2143,2152)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2259..2505
                     /region_name="Laminin_I"
                     /note="Laminin Domain I; pfam06008"
                     /db_xref="CDD:310534"
     Region          2662..2801
                     /region_name="Laminin_II"
                     /note="Laminin Domain II; pfam06009"
                     /db_xref="CDD:368703"
     Region          2777..2948
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2983..3135
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3190..3313
                     /region_name="Laminin_G_2"
                     /note="Laminin G domain; pfam02210"
                     /db_xref="CDD:460494"
     Region          3435..3584
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          3609..3763
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     CDS             1..3786
                     /gene="LanA"
                     /coded_by="XM_034321837.2:198..11558"
                     /db_xref="GeneID:117603081"
ORIGIN      
        1 mflknwnisp ggcgketlnh rwsrwtrsri rlatptaplp qslpvlgdlr pvslhreavq
       61 apsqhlrvsl lphspaerkt aregqkkgis qprvlyfvrt rchkgkqeee qggadsgrqd
      121 lstvrmarwl livslasvlv hqtrneiltp pyfnlaegke iiasatcgvd tpgpelyckl
      181 vgasadqhed inliqgqvcd ycdsdnpekr hppeyavdgm etwwqsppls rgmkynevnl
      241 tinlgqefhv ayvyvkmans prpglwvlek skdygktwsp wqyfsdtand cltyfgvdsy
      301 kpitrddsvi ctteysqivp leggeipisi lnnrpsakhy fnstllqewt ratnvrfrfl
      361 rtknllghlm slvredptvt rryfysikdi siggrcmcng hadtcdildp kmpkklvcrc
      421 qhntcgpqca tcckgfeqkk wrqstafkkf tcepcncfgh sdeceydtev dekhlsldih
      481 gnyegggvcq ncrdntegin cnrckpkfyr pqdkslnatd vcqpcqcdvf ystgncadat
      541 gkcecrkeyt apdcdscsfg yygypncvpc qchlngtdgn yceaidgscp ckpnyaghyc
      601 nlcaegyynf peclpcecnl pssldevcdv vsgnctcknn fggrtcdice hgyynypfct
      661 ycncdtrgtv pgicnksnga clckegygga rcdqcisgyy gypncrpcnc stigsssisc
      721 datgkctclt nfagrtcnqc spgyymypec iacncdshgs igascdaegk cqcrenfdga
      781 rcnqckegfy nfptcegcnc dpagvletfq gcgslpagel cqckdrvegr icnqckplfw
      841 nlqpynpqgc edcqcnipgv ignigecdtk ngqcickpgv mgrscdqcid gtynleernf
      901 fgctdcacdi ggavnsvcdk qtgqcqcqpr vtgltckepl kahyfptlhq fqyeaedgrt
      961 psnspvrygf teehfpgysw kgyavfsalq deiildvyiq ksslyrmvlk yvnpnnepil
     1021 gtititpenp seveqqfkvn fkptvkpsfv tvagpqgnlp spmvmnpghw sisiatkksl
     1081 fldyfvllps eyyeatiltq dvnipcevgs kglcrhygyp nltmfdsvhg aggflnennv
     1141 riplteyfte rdilrevnrd elplindrqe eihfdlrisk pvphvllvly vtpkdensts
     1201 tllieantvg kgkatlypck ytsicrqvvt dnygrvaamn fpsnyislil tgepssnvai
     1261 dsvvaipyne wsldyvkpks icvrkdgkcv kgqfpgaaea kkiefessst ipesenrpyg
     1321 iydnaskliy lddknttvdi hakvvqpgdy tfivqyyqpd ypeyeldvll qngkfyeakm
     1381 svphcpsnsg crsvvkqvdg nirfqlienf mitfkestgk giwldyilvv pteqynekil
     1441 kkiqfdqtke fikkcgynhf hiniteegfc rdsifsitas ynnralpcnc didgtisfec
     1501 ekfggqcpck pniigrrcgi cktgfygfpn ckpcncpsia lcepetgaci cpsrvtgerc
     1561 nqceagtygf hpiigceecn csplgviggd lqcdlfngsc kckenvvgrq cdkceagysq
     1621 fphcercdcd trgttseicd qytaecfcka nvqgsacdvc kegtfniqes neegctkcfc
     1681 fgkttrcvsa nlyrthvsdm tgwelvvsse kggnltylmt ipqeynetsi vvglttndtf
     1741 gqiiyfsasd tylgkkltsy ggslrytvfy stgpfgnavs aadvilqgan ttlfyyadeq
     1801 ppsflnfets velievnflt qnrlsatreq lmmvledlrg iyiratywnp sisatlshas
     1861 levtteyyss qynvpassve qcqcppnyqg lsceecapgy yrvqsgshgg ycvrcecngh
     1921 adtcdvetgi cleckdgttg dhcefceegy ygnatggtpt dclicacplp ipsnnfatgc
     1981 evneegskis cdcvtgyyga rcqvcasgyy gnpevsgdyc kpcqcsgnid tnqvgscdsi
     2041 tgqclqclnn sygeacslck pgyfgdaier kdcrscacer cgmkecdshs gqcfchenvv
     2101 getcdrcadd hygydtcegc kacecgtase rsqcdevtgq ckckpgvtgr rcdqcipgyw
     2161 nygpegcasc gcntgysvgv scntttgqcs clpgvigekc dhcpyrnvli pgqgcfacds
     2221 ctgdlldvtd elselldpvf reystvaesy ftnqrlkfin dtvnelypev klldpsrvnf
     2281 lplqqevkrl elevsnqkrg idytaedsek wkeaaentlk dmnfleddvi geidfvkstv
     2341 aeveslalni elgtgakles slkeatdilr kiqevsfvgf rdratdqadq anilvsemld
     2401 ynvpvsnlsa ladnlsnkik nvtgkmddll eitwkaqela skvdrlnken riaaetgnfd
     2461 ivknatsean ddliagegln rnasryleea nrnveilktn aiddsteilk rtimendqql
     2521 vdlivpvqna hdhaerlynr sldldssltd trntdavkav sayknietai qaareaadda
     2581 mdaaenatsl sdgigqrvln sqngsfylle sarnmlgktq gtpenqlsda qadasyitiq
     2641 nrrnrelldn idkalsnips psstvaqdam nqvvdveqki kqsvnnmygi vdqipedlkn
     2701 akqlsknise ssrdisqakk qldmidkyfp nmtellnsln tnqksidttg ndlqgkieal
     2761 knkianarel adrfkvgltf yrnttlelkn penlpllats tkvslyfrtn ktngfllylg
     2821 neengklprs ktrdfmalli esgypvliid lgsgpekiin nklvsdnvwr qiivdrtgkn
     2881 vklivredig egkdrlyeka hvlpgsyyif nvdpehsklf vggypssfnm qdavtassfe
     2941 gemedlvige dtpvsfwnfi dgennqkaai erdklinfqp stgyrfdkhg failskrsfq
     3001 ispdskkfsi klnfktfapd gliylmgkgr qflslemrdg hvlyqydlgd geislrstnv
     3061 yndgswhnle alrfekkgvl kvdgvhvvnd ratgnsktlv ssdtiyfggy ppnakhpyqp
     3121 vthegfegci ddvvimdtiv dltrniqafg vapgcpvrfa smvffeentp gyvrwhnisv
     3181 pnalqvnlkf ktlandglif yvtdhekgvv sylslidgvl afnsqglelk tgingmkfnd
     3241 newhvitath sgdslkldid dtknyttiea pptllipsgs lyigglpaaf gipptgamtp
     3301 fvgcigdatl ngviinfant terphaflgk ckggdpsslp pvvepepdgv ppplptespd
     3361 dnvevstqin iididlskep dedepilegr gnhdeltttm kpttpapihv dqcrlpyypa
     3421 idpdlentwr fgiarnslle yrslngryrn dydfqidvkt maeegiifyt sdhanenlia
     3481 lyisdgrlhy tfdcgsgpal lisdkkindn ewhivifkrq gnygelivde nnavtgysqg
     3541 ttnimnvnpp fyvggvipdi sskvqsmigl dksfngclgn fmvngqpvge pinevgvipc
     3601 skrvepglff ypgngtnlfk avdrftvgrt vdiqmdikpr sasghllsvh gkrdyvvlem
     3661 ingtvkflvk takgsietsf eptnpnslcd gnwhniravk qknavllsvd hkaapagigg
     3721 knyagvmskh piyigghpml grklrgstsq aqyvgcitnv hinmrpvtlg peraygqvia
     3781 gvcpti
//
LOCUS       XP_034177748             714 aa            linear   INV 03-SEP-2025
DEFINITION  peptide transporter family 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034177748
VERSION     XP_034177748.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321857.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177748.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..714
                     /product="peptide transporter family 1 isoform X1"
                     /calculated_mol_wt=81034
     Region          31..689
                     /region_name="MFS_SLC15A1_2_like"
                     /note="Solute carrier family 15 members 1 and 2, and
                     similar Major Facilitator Superfamily transporters;
                     cd17347"
                     /db_xref="CDD:340905"
     Site            order(44..45,48..49,52,85,144..145,147..149,152,176,
                     179..180,183,294,297..298,301..303,306,337,341,604..605,
                     609,613,629,632..633,636..637,640)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340905"
     CDS             1..714
                     /gene="LOC117603089"
                     /coded_by="XM_034321857.2:65..2209"
                     /db_xref="GeneID:117603089"
ORIGIN      
        1 mderkasine irdhpdnksd meknqkklky pkavffiisn efcerfsfyg irtiltlyll
       61 dylhykkett itiyhtftml ayffpvigai ladsllgkfr tifylsiiya igqiiftisa
      121 tpvvyslspr efsiagllli aigtggiktc vvafggdqfv lpqqerylst ffsifyfsin
      181 lgslissflt pelrtiscfg dnncyslaff vptvlmllsl vifllgkply kilepkgnvi
      241 vnvskcisha iykkirtkgv krdhwldcad deydrsfind iksafqvmkl flpipifwal
      301 fdqqgtrwtm qarrmngeig nfilqpdqmq vvnpllvltf iplfeiciyp lmnkiglrtp
      361 lrkltiggll aslsffasai velelektdp vlpstgvaql rlfntlncpv emqivnesrf
      421 vlqslemwen kyihmdsstk elnyvadysk cpgirslskn gkitvkeaia nswvitpegl
      481 sysykdsvqk ldsfepavrg liyvnspgks vdiefikdgt vlfrlpinst sfhvteflni
      541 kvntydirmn givksnvpfr tggvytlvgy vnenesnlnv itvtepnsmh ilwllpqyii
      601 itmgevmfsv tglefaftqa pismksllqa twllttafgn livviitvag sstffsrmfy
      661 efilfaglml vaififavma yfykyveqpe eesiteeihm dtkngavnes ykdd
//
LOCUS       XP_034177758             697 aa            linear   INV 03-SEP-2025
DEFINITION  peptide transporter family 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034177758
VERSION     XP_034177758.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321867.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177758.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="peptide transporter family 1 isoform X2"
                     /calculated_mol_wt=78983
     Region          14..672
                     /region_name="MFS_SLC15A1_2_like"
                     /note="Solute carrier family 15 members 1 and 2, and
                     similar Major Facilitator Superfamily transporters;
                     cd17347"
                     /db_xref="CDD:340905"
     Site            order(27..28,31..32,35,68,127..128,130..132,135,159,
                     162..163,166,277,280..281,284..286,289,320,324,587..588,
                     592,596,612,615..616,619..620,623)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340905"
     CDS             1..697
                     /gene="LOC117603089"
                     /coded_by="XM_034321867.2:116..2209"
                     /db_xref="GeneID:117603089"
ORIGIN      
        1 miegnterek lkypkavffi isnefcerfs fygirtiltl ylldylhykk ettitiyhtf
       61 tmlayffpvi gailadsllg kfrtifylsi iyaigqiift isatpvvysl sprefsiagl
      121 lliaigtggi ktcvvafggd qfvlpqqery lstffsifyf sinlgsliss fltpelrtis
      181 cfgdnncysl affvptvlml lslvifllgk plykilepkg nvivnvskci shaiykkirt
      241 kgvkrdhwld caddeydrsf indiksafqv mklflpipif walfdqqgtr wtmqarrmng
      301 eignfilqpd qmqvvnpllv ltfiplfeic iyplmnkigl rtplrkltig gllaslsffa
      361 saivelelek tdpvlpstgv aqlrlfntln cpvemqivne srfvlqslem wenkyihmds
      421 stkelnyvad yskcpgirsl skngkitvke aianswvitp eglsysykds vqkldsfepa
      481 vrgliyvnsp gksvdiefik dgtvlfrlpi nstsfhvtef lnikvntydi rmngivksnv
      541 pfrtggvytl vgyvnenesn lnvitvtepn smhilwllpq yiiitmgevm fsvtglefaf
      601 tqapismksl lqatwlltta fgnlivviit vagsstffsr mfyefilfag lmlvaififa
      661 vmayfykyve qpeeesitee ihmdtkngav nesykdd
//
LOCUS       XP_034177767             676 aa            linear   INV 03-SEP-2025
DEFINITION  peptide transporter family 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034177767
VERSION     XP_034177767.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321876.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177767.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..676
                     /product="peptide transporter family 1 isoform X3"
                     /calculated_mol_wt=76244
     Region          15..645
                     /region_name="2A1704"
                     /note="Peptide:H+ symporter (also transports b-lactam
                     antibiotics, the antitumor agent, bestatin, and various
                     protease inhibitors); TIGR00926"
                     /db_xref="CDD:273343"
     CDS             1..676
                     /gene="LOC117603089"
                     /coded_by="XM_034321876.2:175..2205"
                     /db_xref="GeneID:117603089"
ORIGIN      
        1 mnsardfpst esvvsiltly lldylhykke ttitiyhtft mlayffpvig ailadsllgk
       61 frtifylsii yaigqiifti satpvvysls prefsiagll liaigtggik tcvvafggdq
      121 fvlpqqeryl stffsifyfs inlgslissf ltpelrtisc fgdnncysla ffvptvlmll
      181 slvifllgkp lykilepkgn vivnvskcis haiykkirtk gvkrdhwldc addeydrsfi
      241 ndiksafqvm klflpipifw alfdqqgtrw tmqarrmnge ignfilqpdq mqvvnpllvl
      301 tfiplfeici yplmnkiglr tplrkltigg llaslsffas aivelelekt dpvlpstgva
      361 qlrlfntlnc pvemqivnes rfvlqslemw enkyihmdss tkelnyvady skcpgirsls
      421 kngkitvkea ianswvitpe glsysykdsv qkldsfepav rgliyvnspg ksvdiefikd
      481 gtvlfrlpin stsfhvtefl nikvntydir mngivksnvp frtggvytlv gyvnenesnl
      541 nvitvtepns mhilwllpqy iiitmgevmf svtglefaft qapismksll qatwllttaf
      601 gnlivviitv agsstffsrm fyefilfagl mlvaififav mayfykyveq peeesiteei
      661 hmdtkngavn esykdd
//
LOCUS       XP_034177775             676 aa            linear   INV 03-SEP-2025
DEFINITION  peptide transporter family 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034177775
VERSION     XP_034177775.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321884.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177775.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..676
                     /product="peptide transporter family 1 isoform X3"
                     /calculated_mol_wt=76244
     Region          15..645
                     /region_name="2A1704"
                     /note="Peptide:H+ symporter (also transports b-lactam
                     antibiotics, the antitumor agent, bestatin, and various
                     protease inhibitors); TIGR00926"
                     /db_xref="CDD:273343"
     CDS             1..676
                     /gene="LOC117603089"
                     /coded_by="XM_034321884.2:183..2213"
                     /db_xref="GeneID:117603089"
ORIGIN      
        1 mnsardfpst esvvsiltly lldylhykke ttitiyhtft mlayffpvig ailadsllgk
       61 frtifylsii yaigqiifti satpvvysls prefsiagll liaigtggik tcvvafggdq
      121 fvlpqqeryl stffsifyfs inlgslissf ltpelrtisc fgdnncysla ffvptvlmll
      181 slvifllgkp lykilepkgn vivnvskcis haiykkirtk gvkrdhwldc addeydrsfi
      241 ndiksafqvm klflpipifw alfdqqgtrw tmqarrmnge ignfilqpdq mqvvnpllvl
      301 tfiplfeici yplmnkiglr tplrkltigg llaslsffas aivelelekt dpvlpstgva
      361 qlrlfntlnc pvemqivnes rfvlqslemw enkyihmdss tkelnyvady skcpgirsls
      421 kngkitvkea ianswvitpe glsysykdsv qkldsfepav rgliyvnspg ksvdiefikd
      481 gtvlfrlpin stsfhvtefl nikvntydir mngivksnvp frtggvytlv gyvnenesnl
      541 nvitvtepns mhilwllpqy iiitmgevmf svtglefaft qapismksll qatwllttaf
      601 gnlivviitv agsstffsrm fyefilfagl mlvaififav mayfykyveq peeesiteei
      661 hmdtkngavn esykdd
//
LOCUS       XP_034177783             676 aa            linear   INV 03-SEP-2025
DEFINITION  peptide transporter family 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034177783
VERSION     XP_034177783.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321892.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177783.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..676
                     /product="peptide transporter family 1 isoform X3"
                     /calculated_mol_wt=76244
     Region          15..645
                     /region_name="2A1704"
                     /note="Peptide:H+ symporter (also transports b-lactam
                     antibiotics, the antitumor agent, bestatin, and various
                     protease inhibitors); TIGR00926"
                     /db_xref="CDD:273343"
     CDS             1..676
                     /gene="LOC117603089"
                     /coded_by="XM_034321892.2:455..2485"
                     /db_xref="GeneID:117603089"
ORIGIN      
        1 mnsardfpst esvvsiltly lldylhykke ttitiyhtft mlayffpvig ailadsllgk
       61 frtifylsii yaigqiifti satpvvysls prefsiagll liaigtggik tcvvafggdq
      121 fvlpqqeryl stffsifyfs inlgslissf ltpelrtisc fgdnncysla ffvptvlmll
      181 slvifllgkp lykilepkgn vivnvskcis haiykkirtk gvkrdhwldc addeydrsfi
      241 ndiksafqvm klflpipifw alfdqqgtrw tmqarrmnge ignfilqpdq mqvvnpllvl
      301 tfiplfeici yplmnkiglr tplrkltigg llaslsffas aivelelekt dpvlpstgva
      361 qlrlfntlnc pvemqivnes rfvlqslemw enkyihmdss tkelnyvady skcpgirsls
      421 kngkitvkea ianswvitpe glsysykdsv qkldsfepav rgliyvnspg ksvdiefikd
      481 gtvlfrlpin stsfhvtefl nikvntydir mngivksnvp frtggvytlv gyvnenesnl
      541 nvitvtepns mhilwllpqy iiitmgevmf svtglefaft qapismksll qatwllttaf
      601 gnlivviitv agsstffsrm fyefilfagl mlvaififav mayfykyveq peeesiteei
      661 hmdtkngavn esykdd
//
LOCUS       XP_034177791             676 aa            linear   INV 03-SEP-2025
DEFINITION  peptide transporter family 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034177791
VERSION     XP_034177791.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321900.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177791.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..676
                     /product="peptide transporter family 1 isoform X3"
                     /calculated_mol_wt=76244
     Region          15..645
                     /region_name="2A1704"
                     /note="Peptide:H+ symporter (also transports b-lactam
                     antibiotics, the antitumor agent, bestatin, and various
                     protease inhibitors); TIGR00926"
                     /db_xref="CDD:273343"
     CDS             1..676
                     /gene="LOC117603089"
                     /coded_by="XM_034321900.2:440..2470"
                     /db_xref="GeneID:117603089"
ORIGIN      
        1 mnsardfpst esvvsiltly lldylhykke ttitiyhtft mlayffpvig ailadsllgk
       61 frtifylsii yaigqiifti satpvvysls prefsiagll liaigtggik tcvvafggdq
      121 fvlpqqeryl stffsifyfs inlgslissf ltpelrtisc fgdnncysla ffvptvlmll
      181 slvifllgkp lykilepkgn vivnvskcis haiykkirtk gvkrdhwldc addeydrsfi
      241 ndiksafqvm klflpipifw alfdqqgtrw tmqarrmnge ignfilqpdq mqvvnpllvl
      301 tfiplfeici yplmnkiglr tplrkltigg llaslsffas aivelelekt dpvlpstgva
      361 qlrlfntlnc pvemqivnes rfvlqslemw enkyihmdss tkelnyvady skcpgirsls
      421 kngkitvkea ianswvitpe glsysykdsv qkldsfepav rgliyvnspg ksvdiefikd
      481 gtvlfrlpin stsfhvtefl nikvntydir mngivksnvp frtggvytlv gyvnenesnl
      541 nvitvtepns mhilwllpqy iiitmgevmf svtglefaft qapismksll qatwllttaf
      601 gnlivviitv agsstffsrm fyefilfagl mlvaififav mayfykyveq peeesiteei
      661 hmdtkngavn esykdd
//
LOCUS       XP_034177811             636 aa            linear   INV 03-SEP-2025
DEFINITION  peptide transporter family 1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034177811
VERSION     XP_034177811.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321920.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177811.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="peptide transporter family 1 isoform X4"
                     /calculated_mol_wt=71549
     Region          1..605
                     /region_name="2A1704"
                     /note="Peptide:H+ symporter (also transports b-lactam
                     antibiotics, the antitumor agent, bestatin, and various
                     protease inhibitors); TIGR00926"
                     /db_xref="CDD:273343"
     CDS             1..636
                     /gene="LOC117603089"
                     /coded_by="XM_034321920.2:193..2103"
                     /db_xref="GeneID:117603089"
ORIGIN      
        1 mlayffpvig ailadsllgk frtifylsii yaigqiifti satpvvysls prefsiagll
       61 liaigtggik tcvvafggdq fvlpqqeryl stffsifyfs inlgslissf ltpelrtisc
      121 fgdnncysla ffvptvlmll slvifllgkp lykilepkgn vivnvskcis haiykkirtk
      181 gvkrdhwldc addeydrsfi ndiksafqvm klflpipifw alfdqqgtrw tmqarrmnge
      241 ignfilqpdq mqvvnpllvl tfiplfeici yplmnkiglr tplrkltigg llaslsffas
      301 aivelelekt dpvlpstgva qlrlfntlnc pvemqivnes rfvlqslemw enkyihmdss
      361 tkelnyvady skcpgirsls kngkitvkea ianswvitpe glsysykdsv qkldsfepav
      421 rgliyvnspg ksvdiefikd gtvlfrlpin stsfhvtefl nikvntydir mngivksnvp
      481 frtggvytlv gyvnenesnl nvitvtepns mhilwllpqy iiitmgevmf svtglefaft
      541 qapismksll qatwllttaf gnlivviitv agsstffsrm fyefilfagl mlvaififav
      601 mayfykyveq peeesiteei hmdtkngavn esykdd
//
LOCUS       XP_076546184             668 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 53 member 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546184
VERSION     XP_076546184.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690069.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="solute carrier family 53 member 1 isoform X1"
                     /calculated_mol_wt=78172
     Region          2..161
                     /region_name="SPX_XPR1_like"
                     /note="SPX domain of the xenotropic and polytropic
                     retrovirus receptor 1 (XPR1) and related proteins;
                     cd14477"
                     /db_xref="CDD:269898"
     Region          262..602
                     /region_name="EXS"
                     /note="EXS family; pfam03124"
                     /db_xref="CDD:460816"
     CDS             1..668
                     /gene="LOC117603120"
                     /coded_by="XM_076690069.1:38..2044"
                     /db_xref="GeneID:117603120"
ORIGIN      
        1 mkftehlsah itpewrkqyi nyeemkally aaveqapaad iteshilery fnkfdeqffh
       61 ycdkelakin tfyseklaea trrfaslnne lseilsvsed kqgnrkiryr nnilqkkpvs
      121 arklqelkla fsefylflil lqnyqnlnft gfrkilkkhd kllnidigak wraehvdtal
      181 fhthkdidrl iaetealvtr dlehgdrqra mkrlrvpplg eqlspwitfk vglfsgafvv
      241 lliavilsga qykndnnnwr vlcrlyrgpl lmiqflflmg invygwrssg vnhvlifeld
      301 prnhlseqhi iemasicglv wslsvlgfly setlgippfv qpillytlla vflfnptktl
      361 ryearfwalr vlgrifcapf fyvgfadfwl adqlnslhtv fldfqyfvcf yvqnsswtdv
      421 tdaetcimre lsmrpfvvcl pawfrfaqcl rryrdtketf phlanaakya tsffvvvfsy
      481 lhltnskyyv mstenpyfyl witasimssc faytwdvkld wglfdnnage nkflreeivy
      541 sspyyyyfai iedfvlrfgw afslsltemg yvhadlmvsi vaplevfrrf iwnffrlene
      601 hlnncgkfra vrdisvapvd csdqtqilrm mdasdgvinr krkqkvtdks kplrasskse
      661 slicdlvs
//
LOCUS       XP_076546185             668 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 53 member 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546185
VERSION     XP_076546185.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690070.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="solute carrier family 53 member 1 isoform X1"
                     /calculated_mol_wt=78172
     Region          2..161
                     /region_name="SPX_XPR1_like"
                     /note="SPX domain of the xenotropic and polytropic
                     retrovirus receptor 1 (XPR1) and related proteins;
                     cd14477"
                     /db_xref="CDD:269898"
     Region          262..602
                     /region_name="EXS"
                     /note="EXS family; pfam03124"
                     /db_xref="CDD:460816"
     CDS             1..668
                     /gene="LOC117603120"
                     /coded_by="XM_076690070.1:38..2044"
                     /db_xref="GeneID:117603120"
ORIGIN      
        1 mkftehlsah itpewrkqyi nyeemkally aaveqapaad iteshilery fnkfdeqffh
       61 ycdkelakin tfyseklaea trrfaslnne lseilsvsed kqgnrkiryr nnilqkkpvs
      121 arklqelkla fsefylflil lqnyqnlnft gfrkilkkhd kllnidigak wraehvdtal
      181 fhthkdidrl iaetealvtr dlehgdrqra mkrlrvpplg eqlspwitfk vglfsgafvv
      241 lliavilsga qykndnnnwr vlcrlyrgpl lmiqflflmg invygwrssg vnhvlifeld
      301 prnhlseqhi iemasicglv wslsvlgfly setlgippfv qpillytlla vflfnptktl
      361 ryearfwalr vlgrifcapf fyvgfadfwl adqlnslhtv fldfqyfvcf yvqnsswtdv
      421 tdaetcimre lsmrpfvvcl pawfrfaqcl rryrdtketf phlanaakya tsffvvvfsy
      481 lhltnskyyv mstenpyfyl witasimssc faytwdvkld wglfdnnage nkflreeivy
      541 sspyyyyfai iedfvlrfgw afslsltemg yvhadlmvsi vaplevfrrf iwnffrlene
      601 hlnncgkfra vrdisvapvd csdqtqilrm mdasdgvinr krkqkvtdks kplrasskse
      661 slicdlvs
//
LOCUS       XP_034177825             668 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 53 member 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177825
VERSION     XP_034177825.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321934.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="solute carrier family 53 member 1 isoform X1"
                     /calculated_mol_wt=78172
     Region          2..161
                     /region_name="SPX_XPR1_like"
                     /note="SPX domain of the xenotropic and polytropic
                     retrovirus receptor 1 (XPR1) and related proteins;
                     cd14477"
                     /db_xref="CDD:269898"
     Region          262..602
                     /region_name="EXS"
                     /note="EXS family; pfam03124"
                     /db_xref="CDD:460816"
     CDS             1..668
                     /gene="LOC117603120"
                     /coded_by="XM_034321934.2:38..2044"
                     /db_xref="GeneID:117603120"
ORIGIN      
        1 mkftehlsah itpewrkqyi nyeemkally aaveqapaad iteshilery fnkfdeqffh
       61 ycdkelakin tfyseklaea trrfaslnne lseilsvsed kqgnrkiryr nnilqkkpvs
      121 arklqelkla fsefylflil lqnyqnlnft gfrkilkkhd kllnidigak wraehvdtal
      181 fhthkdidrl iaetealvtr dlehgdrqra mkrlrvpplg eqlspwitfk vglfsgafvv
      241 lliavilsga qykndnnnwr vlcrlyrgpl lmiqflflmg invygwrssg vnhvlifeld
      301 prnhlseqhi iemasicglv wslsvlgfly setlgippfv qpillytlla vflfnptktl
      361 ryearfwalr vlgrifcapf fyvgfadfwl adqlnslhtv fldfqyfvcf yvqnsswtdv
      421 tdaetcimre lsmrpfvvcl pawfrfaqcl rryrdtketf phlanaakya tsffvvvfsy
      481 lhltnskyyv mstenpyfyl witasimssc faytwdvkld wglfdnnage nkflreeivy
      541 sspyyyyfai iedfvlrfgw afslsltemg yvhadlmvsi vaplevfrrf iwnffrlene
      601 hlnncgkfra vrdisvapvd csdqtqilrm mdasdgvinr krkqkvtdks kplrasskse
      661 slicdlvs
//
LOCUS       XP_076546186             668 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 53 member 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546186
VERSION     XP_076546186.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690071.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="solute carrier family 53 member 1 isoform X1"
                     /calculated_mol_wt=78172
     Region          2..161
                     /region_name="SPX_XPR1_like"
                     /note="SPX domain of the xenotropic and polytropic
                     retrovirus receptor 1 (XPR1) and related proteins;
                     cd14477"
                     /db_xref="CDD:269898"
     Region          262..602
                     /region_name="EXS"
                     /note="EXS family; pfam03124"
                     /db_xref="CDD:460816"
     CDS             1..668
                     /gene="LOC117603120"
                     /coded_by="XM_076690071.1:38..2044"
                     /db_xref="GeneID:117603120"
ORIGIN      
        1 mkftehlsah itpewrkqyi nyeemkally aaveqapaad iteshilery fnkfdeqffh
       61 ycdkelakin tfyseklaea trrfaslnne lseilsvsed kqgnrkiryr nnilqkkpvs
      121 arklqelkla fsefylflil lqnyqnlnft gfrkilkkhd kllnidigak wraehvdtal
      181 fhthkdidrl iaetealvtr dlehgdrqra mkrlrvpplg eqlspwitfk vglfsgafvv
      241 lliavilsga qykndnnnwr vlcrlyrgpl lmiqflflmg invygwrssg vnhvlifeld
      301 prnhlseqhi iemasicglv wslsvlgfly setlgippfv qpillytlla vflfnptktl
      361 ryearfwalr vlgrifcapf fyvgfadfwl adqlnslhtv fldfqyfvcf yvqnsswtdv
      421 tdaetcimre lsmrpfvvcl pawfrfaqcl rryrdtketf phlanaakya tsffvvvfsy
      481 lhltnskyyv mstenpyfyl witasimssc faytwdvkld wglfdnnage nkflreeivy
      541 sspyyyyfai iedfvlrfgw afslsltemg yvhadlmvsi vaplevfrrf iwnffrlene
      601 hlnncgkfra vrdisvapvd csdqtqilrm mdasdgvinr krkqkvtdks kplrasskse
      661 slicdlvs
//
LOCUS       XP_034177834             650 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 53 member 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177834
VERSION     XP_034177834.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321943.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..650
                     /product="solute carrier family 53 member 1 isoform X2"
                     /calculated_mol_wt=76521
     Region          2..161
                     /region_name="SPX_XPR1_like"
                     /note="SPX domain of the xenotropic and polytropic
                     retrovirus receptor 1 (XPR1) and related proteins;
                     cd14477"
                     /db_xref="CDD:269898"
     Region          262..602
                     /region_name="EXS"
                     /note="EXS family; pfam03124"
                     /db_xref="CDD:460816"
     CDS             1..650
                     /gene="LOC117603120"
                     /coded_by="XM_034321943.2:38..1990"
                     /db_xref="GeneID:117603120"
ORIGIN      
        1 mkftehlsah itpewrkqyi nyeemkally aaveqapaad iteshilery fnkfdeqffh
       61 ycdkelakin tfyseklaea trrfaslnne lseilsvsed kqgnrkiryr nnilqkkpvs
      121 arklqelkla fsefylflil lqnyqnlnft gfrkilkkhd kllnidigak wraehvdtal
      181 fhthkdidrl iaetealvtr dlehgdrqra mkrlrvpplg eqlspwitfk vglfsgafvv
      241 lliavilsga qykndnnnwr vlcrlyrgpl lmiqflflmg invygwrssg vnhvlifeld
      301 prnhlseqhi iemasicglv wslsvlgfly setlgippfv qpillytlla vflfnptktl
      361 ryearfwalr vlgrifcapf fyvgfadfwl adqlnslhtv fldfqyfvcf yvqnsswtdv
      421 tdaetcimre lsmrpfvvcl pawfrfaqcl rryrdtketf phlanaakya tsffvvvfsy
      481 lhltnskyyv mstenpyfyl witasimssc faytwdvkld wglfdnnage nkflreeivy
      541 sspyyyyfai iedfvlrfgw afslsltemg yvhadlmvsi vaplevfrry iwnyfrlene
      601 hlynvgkfra vrdisigpyr hteddepqta iclmdrindd cdqlitcrnr
//
LOCUS       XP_034177843             650 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 53 member 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177843
VERSION     XP_034177843.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321952.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..650
                     /product="solute carrier family 53 member 1 isoform X2"
                     /calculated_mol_wt=76521
     Region          2..161
                     /region_name="SPX_XPR1_like"
                     /note="SPX domain of the xenotropic and polytropic
                     retrovirus receptor 1 (XPR1) and related proteins;
                     cd14477"
                     /db_xref="CDD:269898"
     Region          262..602
                     /region_name="EXS"
                     /note="EXS family; pfam03124"
                     /db_xref="CDD:460816"
     CDS             1..650
                     /gene="LOC117603120"
                     /coded_by="XM_034321952.2:38..1990"
                     /db_xref="GeneID:117603120"
ORIGIN      
        1 mkftehlsah itpewrkqyi nyeemkally aaveqapaad iteshilery fnkfdeqffh
       61 ycdkelakin tfyseklaea trrfaslnne lseilsvsed kqgnrkiryr nnilqkkpvs
      121 arklqelkla fsefylflil lqnyqnlnft gfrkilkkhd kllnidigak wraehvdtal
      181 fhthkdidrl iaetealvtr dlehgdrqra mkrlrvpplg eqlspwitfk vglfsgafvv
      241 lliavilsga qykndnnnwr vlcrlyrgpl lmiqflflmg invygwrssg vnhvlifeld
      301 prnhlseqhi iemasicglv wslsvlgfly setlgippfv qpillytlla vflfnptktl
      361 ryearfwalr vlgrifcapf fyvgfadfwl adqlnslhtv fldfqyfvcf yvqnsswtdv
      421 tdaetcimre lsmrpfvvcl pawfrfaqcl rryrdtketf phlanaakya tsffvvvfsy
      481 lhltnskyyv mstenpyfyl witasimssc faytwdvkld wglfdnnage nkflreeivy
      541 sspyyyyfai iedfvlrfgw afslsltemg yvhadlmvsi vaplevfrry iwnyfrlene
      601 hlynvgkfra vrdisigpyr hteddepqta iclmdrindd cdqlitcrnr
//
LOCUS       XP_034177853             588 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 53 member 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177853
VERSION     XP_034177853.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321962.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="solute carrier family 53 member 1 isoform X3"
                     /calculated_mol_wt=68906
     Region          2..161
                     /region_name="SPX_XPR1_like"
                     /note="SPX domain of the xenotropic and polytropic
                     retrovirus receptor 1 (XPR1) and related proteins;
                     cd14477"
                     /db_xref="CDD:269898"
     Region          262..587
                     /region_name="EXS"
                     /note="EXS family; pfam03124"
                     /db_xref="CDD:460816"
     CDS             1..588
                     /gene="LOC117603120"
                     /coded_by="XM_034321962.2:38..1804"
                     /db_xref="GeneID:117603120"
ORIGIN      
        1 mkftehlsah itpewrkqyi nyeemkally aaveqapaad iteshilery fnkfdeqffh
       61 ycdkelakin tfyseklaea trrfaslnne lseilsvsed kqgnrkiryr nnilqkkpvs
      121 arklqelkla fsefylflil lqnyqnlnft gfrkilkkhd kllnidigak wraehvdtal
      181 fhthkdidrl iaetealvtr dlehgdrqra mkrlrvpplg eqlspwitfk vglfsgafvv
      241 lliavilsga qykndnnnwr vlcrlyrgpl lmiqflflmg invygwrssg vnhvlifeld
      301 prnhlseqhi iemasicglv wslsvlgfly setlgippfv qpillytlla vflfnptktl
      361 ryearfwalr vlgrifcapf fyvgfadfwl adqlnslhtv fldfqyfvcf yvqnsswtdv
      421 tdaetcimre lsmrpfvvcl pawfrfaqcl rryrdtketf phlanaakya tsffvvvfsy
      481 lhltnskyyv mstenpyfyl witasimssc faytwdvkld wglfdnnage nkflreeivy
      541 sspyyyyfai iedfvlrfgw afslsltemg yvhadlmvsi vaplevfs
//
LOCUS       XP_034177863             564 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 303a1 [Osmia lignaria lignaria].
ACCESSION   XP_034177863
VERSION     XP_034177863.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321972.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177863.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="putative cytochrome P450 303a1"
                     /calculated_mol_wt=64273
     Region          64..558
                     /region_name="CYP15A1-like"
                     /note="cytochrome P450 family 15, subfamily A, polypeptide
                     1, and similar cytochrome P450s; cd20651"
                     /db_xref="CDD:410744"
     Site            order(99,122,126,368,371..372,375..376,379,430,435,
                     439..440,442,465,500..502,506..510,513..514,518)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410744"
     Site            order(102,276..277,279..280,283,312..313,316..317,
                     366..367,370..371,374..375,440,508,552)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:410744"
     CDS             1..564
                     /gene="Cyp303a1"
                     /coded_by="XM_034321972.2:146..1840"
                     /db_xref="GeneID:117603136"
ORIGIN      
        1 mfstvmlvvi lllllfylss rkpkgyppgp kwwpilgcai evahlrqktg yliktfsals
       61 kkygpvvglk igtdrivvln dyksiramlt dedcdgrptg pvyqtrtfgs rkglivvdgn
      121 lwveqrrfvl khlrdfgfgr ksmatiieee amslvghfkk lidsdyneri neskihcnnn
      181 envdgqlykl ikkdnvdtsk stdkyitnkk hlrssdkyan midymeikkm drscdgivvp
      241 mhdafgvtvl ntlwimmagk rfnvddqdlt ylqqilatlm neidmigapf ghfpilrfia
      301 pemsgyksfl eihqklwkfl rdeldghrnt ftpnsprdlm dvyldvlssk nysnsfseaq
      361 llaicvdlfm agsettsksl gfcflnlmln phvqkkahee idrvigrnrp ptledrskmt
      421 ymnaivlesl rifmgrtlnv phralrdtsi sghripkdtm livnferilm geswgdpenf
      481 rperfinesg nivtpqtyfp fsigrhrcmg enlarsnifi itttllqaft fspvpgeekp
      541 ssqdfvdgvt ggpkpfralv slrt
//
LOCUS       XP_034177885             535 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-1,3-mannosyl-glycoprotein
            4-beta-N-acetylglucosaminyltransferase a [Osmia lignaria lignaria].
ACCESSION   XP_034177885
VERSION     XP_034177885.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..535
                     /product="alpha-1,3-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase a"
                     /calculated_mol_wt=61184
     Region          102..381
                     /region_name="Glyco_transf_54"
                     /note="N-Acetylglucosaminyltransferase-IV (GnT-IV)
                     conserved region; pfam04666"
                     /db_xref="CDD:461384"
     CDS             1..535
                     /gene="Mgat4a"
                     /coded_by="XM_034321994.2:533..2140"
                     /db_xref="GeneID:117603147"
ORIGIN      
        1 mmphfvaltp irrrcliils ivlvpcailn llspselqee tilqnniael qaklehlhak
       61 yvtsqeeinl lshqllqlie nnhilpdlqf llnnvtsnvt niklpsiynf lphflndpgs
      121 lrpafvqskg rtgvsmvlgv ptvkrevqsy llatlknlld rmspvettdt liivliaetd
      181 leyvtyvakq ievqfptefe agvidvisps asyypdlskl rdtlgddhqr vvwrskqnld
      241 faflmsyaqt kgtfyvqled dilakknfis tmksfalqki atkenwfvld fcqlgfigkl
      301 fkcaelpwli qfflmfhndk pvdwlldhlv stkvcsldkd skhckmakae lwvhykpslf
      361 qhigthsslk gkvqklkdkq fgkitlyyah enpeatvetq ikpykqytlq kaykgesffw
      421 gllpqpgdhl kfkfshpifi krylfrsgnp ehpsdrfynt tvevftelss slnrnsndit
      481 edgyliigkf dalgiaqgtv drklgkilvl rltvhsesen wailseihiv edhps
//
LOCUS       XP_034177944             495 aa            linear   INV 03-SEP-2025
DEFINITION  splicing regulatory protein 54 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177944
VERSION     XP_034177944.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322053.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="splicing regulatory protein 54 isoform X1"
                     /calculated_mol_wt=56783
     Region          8..84
                     /region_name="RRM_SRSF11_SREK1"
                     /note="RNA recognition motif (RRM) found in
                     serine/arginine-rich splicing factor 11 (SRSF11), splicing
                     regulatory glutamine/lysine-rich protein 1 (SREK1) and
                     similar proteins; cd12259"
                     /db_xref="CDD:409704"
     Region          166..251
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..495
                     /gene="Srp54"
                     /coded_by="XM_034322053.2:181..1668"
                     /db_xref="GeneID:117603175"
ORIGIN      
        1 mavgstkvvq vtniapqatk dqmqtlfgyl gkiedirlyp tirdvavpvq sricyikfhd
       61 qgcvsvaqhm tntvfidral ivipyqngdi pdeqralelt nngtvvpvaf fsglypsepk
      121 lppnvvnaie gippnhvitt mdpkleangl ppyphlpghl dsrrieeirr tlvlanldps
      181 vttdhlldff stnnvevkyl rmctrdsdae hyalvelseq aavvsallln gkvlmdrpik
      241 iyhstqaiak peaksneaaq keieeamsrv keahnlisaa idpmigmlsk dkrsrsgsrs
      301 rksrsrsrgr srrsrsrkrs rsrhrrsrsr hrrrsrsrsk rsrskerrrk spsrrrsssr
      361 grhrsrsrsr rsrsrrsrsr skdrkkrssp rrrsrsrsrs krsksksrrs rsksksrssk
      421 skyseksrdk dkdkerrskd ksdngkkndn dkadkeksek ksskekksek eskseeknrn
      481 tsensdtkdk tesen
//
LOCUS       XP_034177953             490 aa            linear   INV 03-SEP-2025
DEFINITION  splicing regulatory protein 54 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177953
VERSION     XP_034177953.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322062.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="splicing regulatory protein 54 isoform X2"
                     /calculated_mol_wt=56232
     Region          8..84
                     /region_name="RRM_SRSF11_SREK1"
                     /note="RNA recognition motif (RRM) found in
                     serine/arginine-rich splicing factor 11 (SRSF11), splicing
                     regulatory glutamine/lysine-rich protein 1 (SREK1) and
                     similar proteins; cd12259"
                     /db_xref="CDD:409704"
     Region          161..246
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..490
                     /gene="Srp54"
                     /coded_by="XM_034322062.2:181..1653"
                     /db_xref="GeneID:117603175"
ORIGIN      
        1 mavgstkvvq vtniapqatk dqmqtlfgyl gkiedirlyp tirdvavpvq sricyikfhd
       61 qgcvsvaqhm tntvfidral ivipyqngdi pdeqralelt nngtvvpgly psepklppnv
      121 vnaiegippn hvittmdpkl eanglppyph lpghldsrri eeirrtlvla nldpsvttdh
      181 lldffstnnv evkylrmctr dsdaehyalv elseqaavvs alllngkvlm drpikiyhst
      241 qaiakpeaks neaaqkeiee amsrvkeahn lisaaidpmi gmlskdkrsr sgsrsrksrs
      301 rsrgrsrrsr srkrsrsrhr rsrsrhrrrs rsrskrsrsk errrkspsrr rsssrgrhrs
      361 rsrsrrsrsr rsrsrskdrk krssprrrsr srsrskrsks ksrrsrsksk srsskskyse
      421 ksrdkdkdke rrskdksdng kkndndkadk eksekksske kksekeskse eknrntsens
      481 dtkdktesen
//
LOCUS       XP_034177965             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603185 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177965
VERSION     XP_034177965.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322074.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177965.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC117603185 isoform X1"
                     /calculated_mol_wt=55660
     Region          <313..474
                     /region_name="NPDC1"
                     /note="Neural proliferation differentiation control-1
                     protein (NPDC1); pfam06809"
                     /db_xref="CDD:284276"
     CDS             1..484
                     /gene="LOC117603185"
                     /coded_by="XM_034322074.2:44..1498"
                     /db_xref="GeneID:117603185"
ORIGIN      
        1 mmtptlctct wlllpccvli slstgeldfy rdevpespen ialmeyrark lidliesqnh
       61 hqqhphrapi fsplignpsh kkhhldsrrq kerdllnvlt dkailaeilr kkkakewnlr
      121 qtrynepine lpeereqylp idelnrleyl kfklpylsrk indddvnyrd egyeyeengp
      181 ikeipyeerk fdrgmkeepl lgyhgsysgs fdeqiaandm nvqkphttvp eftyhfydsd
      241 lnerhpfavk pnnpkyyqdg pfffgdndes smeepsrnkt kweqesseie ksqaiheest
      301 rnadskfkdt smitlseleq rtgysplvvp vnhdlnndiy fiaivagcsa aamfalvlia
      361 ltwcrlkrga kaaadieypa ygvtgpnkei spsgdqrlaq saqmyhfqhq kqqiiamenr
      421 vsatrdpgsi seaeseeene egdytvyecp glastgemev knplfhddpt patpaqtnke
      481 edhm
//
LOCUS       XP_034177975             441 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603185 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177975
VERSION     XP_034177975.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322084.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177975.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="uncharacterized protein LOC117603185 isoform X2"
                     /calculated_mol_wt=50859
     Region          <270..431
                     /region_name="NPDC1"
                     /note="Neural proliferation differentiation control-1
                     protein (NPDC1); pfam06809"
                     /db_xref="CDD:284276"
     CDS             1..441
                     /gene="LOC117603185"
                     /coded_by="XM_034322084.2:126..1451"
                     /db_xref="GeneID:117603185"
ORIGIN      
        1 meyrarklid liesqnhhqq hphrapifsp lignpshkkh hldsrrqker dllnvltdka
       61 ilaeilrkkk akewnlrqtr ynepinelpe ereqylpide lnrleylkfk lpylsrkind
      121 ddvnyrdegy eyeengpike ipyeerkfdr gmkeepllgy hgsysgsfde qiaandmnvq
      181 kphttvpeft yhfydsdlne rhpfavkpnn pkyyqdgpff fgdndessme epsrnktkwe
      241 qesseieksq aiheestrna dskfkdtsmi tlseleqrtg ysplvvpvnh dlnndiyfia
      301 ivagcsaaam falvlialtw crlkrgakaa adieypaygv tgpnkeisps gdqrlaqsaq
      361 myhfqhqkqq iiamenrvsa trdpgsisea eseeeneegd ytvyecpgla stgemevknp
      421 lfhddptpat paqtnkeedh m
//
LOCUS       XP_034177983             441 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603185 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177983
VERSION     XP_034177983.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322092.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177983.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="uncharacterized protein LOC117603185 isoform X2"
                     /calculated_mol_wt=50859
     Region          <270..431
                     /region_name="NPDC1"
                     /note="Neural proliferation differentiation control-1
                     protein (NPDC1); pfam06809"
                     /db_xref="CDD:284276"
     CDS             1..441
                     /gene="LOC117603185"
                     /coded_by="XM_034322092.2:90..1415"
                     /db_xref="GeneID:117603185"
ORIGIN      
        1 meyrarklid liesqnhhqq hphrapifsp lignpshkkh hldsrrqker dllnvltdka
       61 ilaeilrkkk akewnlrqtr ynepinelpe ereqylpide lnrleylkfk lpylsrkind
      121 ddvnyrdegy eyeengpike ipyeerkfdr gmkeepllgy hgsysgsfde qiaandmnvq
      181 kphttvpeft yhfydsdlne rhpfavkpnn pkyyqdgpff fgdndessme epsrnktkwe
      241 qesseieksq aiheestrna dskfkdtsmi tlseleqrtg ysplvvpvnh dlnndiyfia
      301 ivagcsaaam falvlialtw crlkrgakaa adieypaygv tgpnkeisps gdqrlaqsaq
      361 myhfqhqkqq iiamenrvsa trdpgsisea eseeeneegd ytvyecpgla stgemevknp
      421 lfhddptpat paqtnkeedh m
//
LOCUS       XP_034177899             481 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome assembly protein METTL17, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034177899
VERSION     XP_034177899.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322008.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177899.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="ribosome assembly protein METTL17,
                     mitochondrial"
                     /calculated_mol_wt=56333
     Region          150..435
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..481
                     /gene="LOC117603151"
                     /coded_by="XM_034322008.2:202..1647"
                     /db_xref="GeneID:117603151"
ORIGIN      
        1 mssrltfvkc kqirwystkq kmkvtdevde llltnqlkhr hhpgissprq vqhpswlcns
       61 ikalagvnni slkqvqqgtk rltsylherq lpkeaddiet kikdikshtr ainingmeep
      121 mnhkellknp qvlkqlkgfi yswksinyda ftslvylfgr sipeysvlyk ifneiythdk
      181 nftpkslfdy gsgigtvmwa asefwnksik eyycvdvsrf mnefseylmk maepkinphh
      241 vfyrqffpaa piptydivvs aysllelpdq ksrleviskl wnktnqylvi veqgstygfr
      301 viteardfil rhgrkdtrgv hvfspcphdl qcpiqiadyk fpcsfkihyq tlplaqksey
      361 kmelysyivl kkgkrsendd qwpriispil krskhvicrm ctasgkfeeq iftvwkngkn
      421 tyrcarssaw gdrlpfkfee ikeseeseds dqfeeikese esknsdqfed sleseesekt
      481 k
//
LOCUS       XP_034177910             481 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome assembly protein METTL17, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034177910
VERSION     XP_034177910.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322019.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177910.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="ribosome assembly protein METTL17,
                     mitochondrial"
                     /calculated_mol_wt=56333
     Region          150..435
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..481
                     /gene="LOC117603151"
                     /coded_by="XM_034322019.2:199..1644"
                     /db_xref="GeneID:117603151"
ORIGIN      
        1 mssrltfvkc kqirwystkq kmkvtdevde llltnqlkhr hhpgissprq vqhpswlcns
       61 ikalagvnni slkqvqqgtk rltsylherq lpkeaddiet kikdikshtr ainingmeep
      121 mnhkellknp qvlkqlkgfi yswksinyda ftslvylfgr sipeysvlyk ifneiythdk
      181 nftpkslfdy gsgigtvmwa asefwnksik eyycvdvsrf mnefseylmk maepkinphh
      241 vfyrqffpaa piptydivvs aysllelpdq ksrleviskl wnktnqylvi veqgstygfr
      301 viteardfil rhgrkdtrgv hvfspcphdl qcpiqiadyk fpcsfkihyq tlplaqksey
      361 kmelysyivl kkgkrsendd qwpriispil krskhvicrm ctasgkfeeq iftvwkngkn
      421 tyrcarssaw gdrlpfkfee ikeseeseds dqfeeikese esknsdqfed sleseesekt
      481 k
//
LOCUS       XP_034177916             481 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome assembly protein METTL17, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034177916
VERSION     XP_034177916.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322025.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177916.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="ribosome assembly protein METTL17,
                     mitochondrial"
                     /calculated_mol_wt=56333
     Region          150..435
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..481
                     /gene="LOC117603151"
                     /coded_by="XM_034322025.2:200..1645"
                     /db_xref="GeneID:117603151"
ORIGIN      
        1 mssrltfvkc kqirwystkq kmkvtdevde llltnqlkhr hhpgissprq vqhpswlcns
       61 ikalagvnni slkqvqqgtk rltsylherq lpkeaddiet kikdikshtr ainingmeep
      121 mnhkellknp qvlkqlkgfi yswksinyda ftslvylfgr sipeysvlyk ifneiythdk
      181 nftpkslfdy gsgigtvmwa asefwnksik eyycvdvsrf mnefseylmk maepkinphh
      241 vfyrqffpaa piptydivvs aysllelpdq ksrleviskl wnktnqylvi veqgstygfr
      301 viteardfil rhgrkdtrgv hvfspcphdl qcpiqiadyk fpcsfkihyq tlplaqksey
      361 kmelysyivl kkgkrsendd qwpriispil krskhvicrm ctasgkfeeq iftvwkngkn
      421 tyrcarssaw gdrlpfkfee ikeseeseds dqfeeikese esknsdqfed sleseesekt
      481 k
//
LOCUS       XP_034177926             481 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome assembly protein METTL17, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034177926
VERSION     XP_034177926.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322035.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177926.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="ribosome assembly protein METTL17,
                     mitochondrial"
                     /calculated_mol_wt=56333
     Region          150..435
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..481
                     /gene="LOC117603151"
                     /coded_by="XM_034322035.2:204..1649"
                     /db_xref="GeneID:117603151"
ORIGIN      
        1 mssrltfvkc kqirwystkq kmkvtdevde llltnqlkhr hhpgissprq vqhpswlcns
       61 ikalagvnni slkqvqqgtk rltsylherq lpkeaddiet kikdikshtr ainingmeep
      121 mnhkellknp qvlkqlkgfi yswksinyda ftslvylfgr sipeysvlyk ifneiythdk
      181 nftpkslfdy gsgigtvmwa asefwnksik eyycvdvsrf mnefseylmk maepkinphh
      241 vfyrqffpaa piptydivvs aysllelpdq ksrleviskl wnktnqylvi veqgstygfr
      301 viteardfil rhgrkdtrgv hvfspcphdl qcpiqiadyk fpcsfkihyq tlplaqksey
      361 kmelysyivl kkgkrsendd qwpriispil krskhvicrm ctasgkfeeq iftvwkngkn
      421 tyrcarssaw gdrlpfkfee ikeseeseds dqfeeikese esknsdqfed sleseesekt
      481 k
//
LOCUS       XP_034177996             418 aa            linear   INV 03-SEP-2025
DEFINITION  tumor susceptibility gene 101 [Osmia lignaria lignaria].
ACCESSION   XP_034177996
VERSION     XP_034177996.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322105.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..418
                     /product="tumor susceptibility gene 101"
                     /calculated_mol_wt=46846
     Region          25..143
                     /region_name="UEV"
                     /note="UEV domain; pfam05743"
                     /db_xref="CDD:399041"
     Region          <262..>409
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          346..405
                     /region_name="Vps23_core"
                     /note="Vps23 core domain; pfam09454"
                     /db_xref="CDD:462803"
     CDS             1..418
                     /gene="TSG101"
                     /coded_by="XM_034322105.2:345..1601"
                     /db_xref="GeneID:117603200"
ORIGIN      
        1 mvkmtppnea kirqslskyq npditkqhvm svlnlynglq yevepfvfnd gsrkelfnlq
       61 gtipvsyegn cynipiciwl mdthpnnapm cyvkptadmy vkvsmfvdhn gkiylpylhd
      121 wlphssdlls liqimivtfg eqppvyakpr kntrsspfsv qgfdpttgsr ahmpgsplpp
      181 ypqnppypdg snvyppymhp assgfpypgs ygsyagnasn flsqgytgsf pyppstqpsp
      241 tvpgttggsg titeehiras llsavedklr rrlkeqfsql laeletlrrt qqeltsgsah
      301 ltelfdrlkk ekfeleknin ilqdkeaele keiaklsdnq sidvdeavtt iaplykqmln
      361 afaeeaated aiyylgeglr cgiidldafl kqvrqlsrrq fmlralmqrc rqkaglag
//
LOCUS       XP_034178009             337 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603207 [Osmia lignaria lignaria].
ACCESSION   XP_034178009
VERSION     XP_034178009.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322118.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="uncharacterized protein LOC117603207"
                     /calculated_mol_wt=38462
     Region          34..117
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          264..322
                     /region_name="FLYWCH"
                     /note="FLYWCH zinc finger domain; pfam04500"
                     /db_xref="CDD:461332"
     CDS             1..337
                     /gene="LOC117603207"
                     /coded_by="XM_034322118.2:172..1185"
                     /db_xref="GeneID:117603207"
ORIGIN      
        1 maqnslsehy slrwnkfcnt itsgflnhlt endlvdvtla vegqllqahk lvlsvcspyf
       61 knifkenpcq hpviilkdmk yteiesllkf myqgemnvkq edlstllkva etlqikgltp
      121 entnstksfs nydgqlnvds dtqniaqshl niinknsnnv eminkrkksc dvikkgtkkr
      181 kqdtdtddtd dlsdenvdnd nqqvqhlmsn dmneftdede dtnvpsikdn dsdekddsad
      241 linaqssren ttqqdvepvi yrlsargrpq lvyegyvynl tsrseglnrs hyrcaeqhrg
      301 ckgkcaviae rfmptgvhdh nhppgyqser dyrkkkv
//
LOCUS       XP_034178025             321 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase NRDP1 elgi isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178025
VERSION     XP_034178025.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322134.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="E3 ubiquitin-protein ligase NRDP1 elgi isoform
                     X1"
                     /calculated_mol_wt=36985
     Region          15..63
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,40,42,45,48,59,62)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          143..321
                     /region_name="USP8_interact"
                     /note="USP8 interacting; pfam08941"
                     /db_xref="CDD:430333"
     CDS             1..321
                     /gene="elgi"
                     /coded_by="XM_034322134.2:808..1773"
                     /db_xref="GeneID:117603211"
ORIGIN      
        1 mgfdvnrfqg evdeelvcpi csgvledpvq vsnmlqapvc ehafcrtcin ewinrqptcp
       61 ldrtpitsaq lravprilrn llarlciscd nimygcqvvv kldslvshle qceynpkrpm
      121 lceqgcslii pknelkdhnc vrelrniihs qqqkladmkr elgeqqlqin ehkreihllk
      181 dfmralrvsn pamraiadqm erdevvrwsa tlprarvtrw ggmistpdel lqtmikrtls
      241 eyncpphvid elmencherk wppglnslet rqnsrrqydn yvckrvpgkq avlvlycdnt
      301 hmpedmmvep glvmifahgi e
//
LOCUS       XP_034178033             315 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase NRDP1 elgi isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178033
VERSION     XP_034178033.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="E3 ubiquitin-protein ligase NRDP1 elgi isoform
                     X2"
                     /calculated_mol_wt=36312
     Region          15..57
                     /region_name="mRING-HC-C3HC3D_Nrdp1"
                     /note="Modified RING finger, HC subclass (C3HC3D-type),
                     found in neuregulin receptor degradation protein-1 (Nrdp1)
                     and similar proteins; cd16634"
                     /db_xref="CDD:438296"
     Region          137..315
                     /region_name="USP8_interact"
                     /note="USP8 interacting; pfam08941"
                     /db_xref="CDD:430333"
     CDS             1..315
                     /gene="elgi"
                     /coded_by="XM_034322142.2:808..1755"
                     /db_xref="GeneID:117603211"
ORIGIN      
        1 mgfdvnrfqg evdeelvcpi csgvledpvq apvcehafcr tcinewinrq ptcpldrtpi
       61 tsaqlravpr ilrnllarlc iscdnimygc qvvvkldslv shleqceynp krpmlceqgc
      121 sliipknelk dhncvrelrn iihsqqqkla dmkrelgeqq lqinehkrei hllkdfmral
      181 rvsnpamrai adqmerdevv rwsatlprar vtrwggmist pdellqtmik rtlseyncpp
      241 hvidelmenc herkwppgln sletrqnsrr qydnyvckrv pgkqavlvly cdnthmpedm
      301 mvepglvmif ahgie
//
LOCUS       XP_034178049             288 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase NRDP1 elgi isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178049
VERSION     XP_034178049.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322158.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="E3 ubiquitin-protein ligase NRDP1 elgi isoform
                     X3"
                     /calculated_mol_wt=33377
     Region          <2..30
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          110..288
                     /region_name="USP8_interact"
                     /note="USP8 interacting; pfam08941"
                     /db_xref="CDD:430333"
     CDS             1..288
                     /gene="elgi"
                     /coded_by="XM_034322158.2:927..1793"
                     /db_xref="GeneID:117603211"
ORIGIN      
        1 mlqapvceha fcrtcinewi nrqptcpldr tpitsaqlra vprilrnlla rlciscdnim
       61 ygcqvvvkld slvshleqce ynpkrpmlce qgcsliipkn elkdhncvre lrniihsqqq
      121 kladmkrelg eqqlqinehk reihllkdfm ralrvsnpam raiadqmerd evvrwsatlp
      181 rarvtrwggm istpdellqt mikrtlseyn cpphvidelm encherkwpp glnsletrqn
      241 srrqydnyvc krvpgkqavl vlycdnthmp edmmvepglv mifahgie
//
LOCUS       XP_034178042             288 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase NRDP1 elgi isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178042
VERSION     XP_034178042.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322151.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="E3 ubiquitin-protein ligase NRDP1 elgi isoform
                     X3"
                     /calculated_mol_wt=33377
     Region          <2..30
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Region          110..288
                     /region_name="USP8_interact"
                     /note="USP8 interacting; pfam08941"
                     /db_xref="CDD:430333"
     CDS             1..288
                     /gene="elgi"
                     /coded_by="XM_034322151.2:915..1781"
                     /db_xref="GeneID:117603211"
ORIGIN      
        1 mlqapvceha fcrtcinewi nrqptcpldr tpitsaqlra vprilrnlla rlciscdnim
       61 ygcqvvvkld slvshleqce ynpkrpmlce qgcsliipkn elkdhncvre lrniihsqqq
      121 kladmkrelg eqqlqinehk reihllkdfm ralrvsnpam raiadqmerd evvrwsatlp
      181 rarvtrwggm istpdellqt mikrtlseyn cpphvidelm encherkwpp glnsletrqn
      241 srrqydnyvc krvpgkqavl vlycdnthmp edmmvepglv mifahgie
//
LOCUS       XP_034178063             171 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 208 [Osmia lignaria lignaria].
ACCESSION   XP_034178063
VERSION     XP_034178063.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="transmembrane protein 208"
                     /calculated_mol_wt=19341
     Region          9..168
                     /region_name="TMEM208_SND2"
                     /note="SRP-independent targeting protein 2/TMEM208;
                     pfam05620"
                     /db_xref="CDD:428546"
     CDS             1..171
                     /gene="LOC117603233"
                     /coded_by="XM_034322172.2:331..846"
                     /db_xref="GeneID:117603233"
ORIGIN      
        1 msikktkaat kgakqiveen kitlsfyqnm iigalgiyft vmmlffnftt lsitltifsg
       61 ivyiasyqfm kyiaqatyse sgqlldsgid lnmeggigeh vkdliiltsg vqvlslisnn
      121 iwllwllvpl rggwmlwkqi lapwffapal eqpeisekkq rklekkmarr h
//
LOCUS       XP_034178075             128 aa            linear   INV 03-SEP-2025
DEFINITION  oligosaccharyltransferase subunit 5 kud [Osmia lignaria lignaria].
ACCESSION   XP_034178075
VERSION     XP_034178075.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322184.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..128
                     /product="oligosaccharyltransferase subunit 5 kud"
                     /calculated_mol_wt=14533
     Region          56..128
                     /region_name="Ost5"
                     /note="Oligosaccharyltransferase subunit 5; pfam05251"
                     /db_xref="CDD:461602"
     CDS             1..128
                     /gene="kud"
                     /coded_by="XM_034322184.2:16..402"
                     /db_xref="GeneID:117603235"
ORIGIN      
        1 msvicvltig syylciilkf atikhnfqsr viftcllnrp rqnilfntmv eiesmtryvs
       61 pinpaifpll avvllgigif ftawffvyev tstkftrdmf kellislvaa ifsgfgvlfl
      121 llwvgiyv
//
LOCUS       XP_034183994             888 aa            linear   INV 03-SEP-2025
DEFINITION  cold shock domain-containing Unr [Osmia lignaria lignaria].
ACCESSION   XP_034183994
VERSION     XP_034183994.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328103.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..888
                     /product="cold shock domain-containing Unr"
                     /calculated_mol_wt=98593
     Region          125..188
                     /region_name="CSP"
                     /note="Cold shock protein domain; smart00357"
                     /db_xref="CDD:214633"
     Site            order(135..140,147..150)
                     /site_type="active"
                     /note="RNA-binding motif [active]"
                     /db_xref="CDD:239905"
     Region          277..337
                     /region_name="S1_like"
                     /note="Ribosomal protein S1-like RNA-binding domain. Found
                     in a wide variety of RNA-associated proteins. Originally
                     identified in S1 ribosomal protein. This superfamily also
                     contains the Cold Shock Domain (CSD), which is a homolog
                     of the S1 domain. Both domains...; cl09927"
                     /db_xref="CDD:471952"
     Site            order(281,288,299,327)
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:239905"
     Site            order(285..291,298..301)
                     /site_type="active"
                     /note="RNA-binding motif [active]"
                     /db_xref="CDD:239905"
     Region          442..509
                     /region_name="CSP"
                     /note="Cold shock protein domain; smart00357"
                     /db_xref="CDD:214633"
     Site            order(451..457,464..467)
                     /site_type="active"
                     /note="RNA-binding motif [active]"
                     /db_xref="CDD:239905"
     Region          611..>654
                     /region_name="CSP"
                     /note="Cold shock protein domain; smart00357"
                     /db_xref="CDD:214633"
     Site            order(620..626,633..636)
                     /site_type="active"
                     /note="RNA-binding motif [active]"
                     /db_xref="CDD:239905"
     Region          769..832
                     /region_name="CSP"
                     /note="Cold shock protein domain; smart00357"
                     /db_xref="CDD:214633"
     Region          846..877
                     /region_name="SUZ-C"
                     /note="SUZ-C motif; pfam12901"
                     /db_xref="CDD:463745"
     CDS             1..888
                     /gene="Unr"
                     /coded_by="XM_034328103.2:1..2667"
                     /db_xref="GeneID:117606087"
ORIGIN      
        1 msnnpqwktf qppimnsdpa ildyksmais spkvitgshq sttniyrngt nysgdhymmd
       61 spprngksti nyagypntkt srngvtrfpf gtftgmegnt gyteepatnn styqdqssnq
      121 gpretgiiek llhsygfiqc cerqarlffh fsqfsgnieh lkigdpvefe mtydrrtgkp
      181 iastvskiap evvlseervt gnvttelqas gdslgrisye nrgecfflpy tkddvegnvt
      241 lragdrvsfq iatnqrgnlg achvrlenpa hpvrycgvvc smkenfgfie radvvkeiff
      301 hfseaksmke elklgddvef iiqtrngkev acnitklppg sivfeevsne vvkgqvlkpl
      361 ergtaarhqn dplpgriryr dtdhseveip fgdkdqkgdf tlrhgdwvqf riatdtrdql
      421 krateilllp esftvsgerr eqgvitalkd gfgfircvdr darlffhfne vldvdreisv
      481 gdeveftviq dpsssfsnnr qsairlkhlp agtvqfetii ekdllgtiiq gingiepgli
      541 gytkdnvqks viffskdcdp kniprlgdkv qfsicqvkrn kelvavdisl vsssgektqn
      601 gnkklngqvc qgfiaalkdg fgfietvnhd keiffhfsnf egdastlevg adiectissg
      661 ngrgnggcva adyvklvprg siprptpvse vldgmvirpl rsanpdqaey aglikvnptn
      721 eddetpeyef rimglvnkre llqagdpvql qvdsaghacn ivavrkkrra tvdavkgpfg
      781 flayeidegk klffhmsevk dhailqpgdq vefvlitnqr tgkssacnvt rlsdagqqrp
      841 erlisrlrtt sledtgpklt ivrqprgpdg trgfsqersq htsgaiqe
//
LOCUS       XP_034184009             730 aa            linear   INV 03-SEP-2025
DEFINITION  na[+]/H[+] hydrogen exchanger 1 [Osmia lignaria lignaria].
ACCESSION   XP_034184009
VERSION     XP_034184009.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328118.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..730
                     /product="na[+]/H[+] hydrogen exchanger 1"
                     /calculated_mol_wt=81028
     Region          <37..>177
                     /region_name="PRK13914"
                     /note="invasion associated endopeptidase"
                     /db_xref="CDD:237555"
     Region          169..>600
                     /region_name="Na_H_Exchanger"
                     /note="Sodium/hydrogen exchanger family; cl01133"
                     /db_xref="CDD:470090"
     CDS             1..730
                     /gene="Nhe1"
                     /coded_by="XM_034328118.2:131..2323"
                     /db_xref="GeneID:117606092"
ORIGIN      
        1 mnlysgsnkl lhrnvvisvi fftlgllttt syaqkisnlk netvtvgnkn itlqilkqtn
       61 lstdastesy vsnsydtsnl klnvstssva ntiphnvttt tvrpehdsyn qttekvttis
      121 tntindkngs nmsdftipsq ssdisnstna stttimpaep vlpdkgsaee ehnssmsiff
      181 vlcvlalgil lihlmletnf qylpesvviv flggaigmii nlmsnqnian wrkeeafspt
      241 afflvllppi ifesgynlhk gnffqnigsi lvfaifgtai safvigagiy llglaqvayk
      301 lsfvesfafg slisavdpva tvaifhaldv dpvlnmlvfg esilndaisi vlttsvlesn
      361 natttseaii lglnrfclmf fasagigvvf alisalllkh vdlrknpsle fglmlvftya
      421 pyvlaegiql sgimailfng ivmshythfn lstvtqitmq qtmrtlafia etcvfaylgl
      481 alfsfrhrve paliiwsiil cligraanif plaflvnrfr ehqitrkmmf imwfsglrga
      541 isyalslhld fsdetrhvii tttliivlft tlifggstmp llkflraekk qksnsrrkrk
      601 dkeislsktr ewgqtidseh lselteeeie vsflqsrirg farwdlkffi pfftrrftqq
      661 elkdcksqmt dltnqwyqai rispmesdde vttastaqlt inlsaenqsq vngkqskgrp
      721 shgisedwsd
//
LOCUS       XP_034184019             645 aa            linear   INV 03-SEP-2025
DEFINITION  SR splicing factor protein kinase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184019
VERSION     XP_034184019.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328128.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..645
                     /product="SR splicing factor protein kinase isoform X1"
                     /calculated_mol_wt=72510
     Region          107..628
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(124..127,130,132,145,147,183,203..206,251,255..256,
                     258,471..472)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..645
                     /gene="SRPK"
                     /coded_by="XM_034328128.2:741..2678"
                     /db_xref="GeneID:117606098"
ORIGIN      
        1 mstktdvnrr vlaiqakkkr hkpskrkgkg nsqgesdtsg aqcskapaar qgqgsgfyqd
       61 asaqrpyssd ngnsssneti edddevysse deeqedssdy ckggyhpvki gdlflnryhv
      121 trklgwghfs tvwlcwdlqd krfvalkvvk sashftetal deikllkdvr dtdpsdpkrn
      181 ktvqllndfk isginglhvc mvfevlghnl lkliiksnyr giprnnvkri irqvlegldy
      241 lhnkckiiht dikpenvlic vdeayirkla ceatelhslg mklpvslist apkefqeptp
      301 nskmsknkkk klkkkakrqn ellkkqmeqi eeleeqnkla nstrengele tnspdqdint
      361 esiedntesk gspdisepsa pslhingvds laggekvenq lpqqsekmdn anlcdveksc
      421 gegalspdhe dadecsegrn lhppeskqlk rasvapldpa lvecdvevki adlgnacwvn
      481 kkftddiqtr qyrslevllg sgydtsadiw stacmafela tgdylfephs gnyycrdedh
      541 lahiiellge iprhialsgk nskayfnkkg elkritglkp wglydvltek ydwspreare
      601 feefltpmle fdpsmrataa eclkhpwlqi kkqisygske ectvq
//
LOCUS       XP_034184028             643 aa            linear   INV 03-SEP-2025
DEFINITION  SR splicing factor protein kinase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184028
VERSION     XP_034184028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..643
                     /product="SR splicing factor protein kinase isoform X2"
                     /calculated_mol_wt=72324
     Region          107..626
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(124..127,130,132,145,147,183,203..206,251,255..256,
                     258,469..470)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..643
                     /gene="SRPK"
                     /coded_by="XM_034328137.2:748..2679"
                     /db_xref="GeneID:117606098"
ORIGIN      
        1 mstktdvnrr vlaiqakkkr hkpskrkgkg nsqgesdtsg aqcskapaar qgqgsgfyqd
       61 asaqrpyssd ngnsssneti edddevysse deeqedssdy ckggyhpvki gdlflnryhv
      121 trklgwghfs tvwlcwdlqd krfvalkvvk sashftetal deikllkdvr dtdpsdpkrn
      181 ktvqllndfk isginglhvc mvfevlghnl lkliiksnyr giprnnvkri irqvlegldy
      241 lhnkckiiht dikpenvlic vdeayirkla ceatelhslg mklpvslist apkefqeptp
      301 nskmsknkkk klkkkakrqn ellkkqmeqi eeleeqnkla nstrengele tnspdqdint
      361 esiedntesk gspdisepsa pslhingvds laggekvenq lpqqsekmdn anlcdveksc
      421 gegalspdhe dadecsrnlh ppeskqlkra svapldpalv ecdvevkiad lgnacwvnkk
      481 ftddiqtrqy rslevllgsg ydtsadiwst acmafelatg dylfephsgn yycrdedhla
      541 hiiellgeip rhialsgkns kayfnkkgel kritglkpwg lydvltekyd wsprearefe
      601 efltpmlefd psmrataaec lkhpwlqikk qisygskeec tvq
//
LOCUS       XP_034190619             599 aa            linear   INV 03-SEP-2025
DEFINITION  U3 small nucleolar RNA-associated protein 6 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034190619
VERSION     XP_034190619.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334728.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..599
                     /product="U3 small nucleolar RNA-associated protein 6
                     homolog"
                     /calculated_mol_wt=70519
     Region          1..>214
                     /region_name="COG5191"
                     /note="Uncharacterized conserved protein, contains HAT
                     (Half-A-TPR) repeat [General function prediction only]"
                     /db_xref="CDD:227518"
     Region          9..91
                     /region_name="U3_assoc_6"
                     /note="U3 small nucleolar RNA-associated protein 6;
                     pfam08640"
                     /db_xref="CDD:462544"
     CDS             1..599
                     /gene="LOC117608946"
                     /coded_by="XM_034334728.2:52..1851"
                     /db_xref="GeneID:117608946"
ORIGIN      
        1 maefvekrce dmipeleqme riklfdrnei rgiakklkey eykiqrkskt kedylryiqy
       61 emdllklikq rrdkfginqk ksdidhtitn kmnrlytdai ykfqddirfw iayikfckhv
      121 rfhnnvshil grmlqvhqdk pkcwhiaarw eleenknkht arqfflrglh ihptsqllft
      181 dafkleldda sgndqknenn sdstsvntdd dmsiglkray iiyqqaskri kdvkfiiell
      241 nitkehsnte klqnkivsdm iqeypheplm wdtmarrele gliqpslsdt aiktdnseqt
      301 slrdritscn kvyqtavkki kteemwslyi eclielnqkt gtlpnfkrkl lktalmqahq
      361 akklkekyyl ywinmfdidk kdeaadkklk eilcvateti pssvslwhar lryllvseee
      421 eaervfpnai qalgekslpl wkmrilhvqa kcpertdeif qaalqghsnv aqelkpvyle
      481 wlvlsksiqs trkvydslcl qppfclelhk kmielelmqp nillknirkc hemstlqfgk
      541 ndtsvwinyi tfemkhgdpk kvgeihvrav ksleasltds fiaeyslika npdslrtnt
//
LOCUS       XP_034190632             549 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608951 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190632
VERSION     XP_034190632.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334741.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190632.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..549
                     /product="uncharacterized protein LOC117608951 isoform X1"
                     /calculated_mol_wt=64400
     CDS             1..549
                     /gene="LOC117608951"
                     /coded_by="XM_034334741.2:312..1961"
                     /db_xref="GeneID:117608951"
ORIGIN      
        1 mevkrtemei frtkktyydd lnetkngsds fweifialcv vliliivfit cyhyihgkii
       61 yiickrcftw lnkkndtddn vtiknesnrg ttrrrsmilk phkkfndqke klyktksyaf
      121 rtplqekkkl lsshtdktit sssnialtkr lflklsvlss dtsnknghdq tsnnwntcne
      181 qctqrnekfy qnshkgeksi cfsdtdvkfl vipvsskksn enrnkeickw nskdnnydir
      241 nntsskliel qyenrdsfsn nvqkyevsts klnlpcsfvs knvatdslsn svvnqldilp
      301 nlslnklkrs tnshigstvs gvsnathlks eensfmdvad satiseidkl qrkksdeynv
      361 yskkmkmkmk ssmdmlrnqi keinserkik sednlmvlsi knentknyfm fkkditsihe
      421 qyiespltsl nhssetneiy delgilfsqy mklqikscrr kycirsrhlk kiqhstrkcq
      481 vrfnyhqiyp ydqrptcncg nicedtydel klmstlpler kniyyemftr rkqflnqpci
      541 linqefisn
//
LOCUS       XP_034190641             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608951 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190641
VERSION     XP_034190641.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334750.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190641.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC117608951 isoform X2"
                     /calculated_mol_wt=56388
     CDS             1..479
                     /gene="LOC117608951"
                     /coded_by="XM_034334750.2:312..1751"
                     /db_xref="GeneID:117608951"
ORIGIN      
        1 mevkrtemei frtkktyydd lnetkngsds fweifialcv vliliivfit cyhyihgkii
       61 yiickrcftw lnkkndtddn vtiknesnrg ttrrrsmilk phkkfndqke klyktksyaf
      121 rtplqgeksi cfsdtdvkfl vipvsskksn enrnkeickw nskdnnydir nntsskliel
      181 qyenrdsfsn nvqkyevsts klnlpcsfvs knvatdslsn svvnqldilp nlslnklkrs
      241 tnshigstvs gvsnathlks eensfmdvad satiseidkl qrkksdeynv yskkmkmkmk
      301 ssmdmlrnqi keinserkik sednlmvlsi knentknyfm fkkditsihe qyiespltsl
      361 nhssetneiy delgilfsqy mklqikscrr kycirsrhlk kiqhstrkcq vrfnyhqiyp
      421 ydqrptcncg nicedtydel klmstlpler kniyyemftr rkqflnqpci linqefisn
//
LOCUS       XP_034190650             453 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608951 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190650
VERSION     XP_034190650.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334759.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190650.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="uncharacterized protein LOC117608951 isoform X3"
                     /calculated_mol_wt=52881
     CDS             1..453
                     /gene="LOC117608951"
                     /coded_by="XM_034334759.2:631..1992"
                     /db_xref="GeneID:117608951"
ORIGIN      
        1 milkphkkfn dqkeklyktk syafrtplqe kkkllsshtd ktitsssnia ltkrlflkls
       61 vlssdtsnkn ghdqtsnnwn tcneqctqrn ekfyqnshkg eksicfsdtd vkflvipvss
      121 kksnenrnke ickwnskdnn ydirnntssk lielqyenrd sfsnnvqkye vstsklnlpc
      181 sfvsknvatd slsnsvvnql dilpnlslnk lkrstnshig stvsgvsnat hlkseensfm
      241 dvadsatise idklqrkksd eynvyskkmk mkmkssmdml rnqikeinse rkiksednlm
      301 vlsiknentk nyfmfkkdit siheqyiesp ltslnhsset neiydelgil fsqymklqik
      361 scrrkycirs rhlkkiqhst rkcqvrfnyh qiypydqrpt cncgnicedt ydelklmstl
      421 plerkniyye mftrrkqfln qpcilinqef isn
//
LOCUS       XP_034184037             454 aa            linear   INV 03-SEP-2025
DEFINITION  suppressor APC domain-containing protein 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184037
VERSION     XP_034184037.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328146.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..454
                     /product="suppressor APC domain-containing protein 2
                     isoform X1"
                     /calculated_mol_wt=51722
     Region          280..361
                     /region_name="Suppressor_APC"
                     /note="Adenomatous polyposis coli tumour suppressor
                     protein; pfam11414"
                     /db_xref="CDD:463275"
     CDS             1..454
                     /gene="LOC117606109"
                     /coded_by="XM_034328146.2:406..1770"
                     /db_xref="GeneID:117606109"
ORIGIN      
        1 maqiqnsgad glpkhfvsam rtlfdimddq ntgfvkfsdi egrwqddgtq glpkgvldsl
       61 kkvtppngll sferfctglk icllqiqaes dskkhdgqqn rppsapilip dsqnkttwts
      121 pntatirpnn aisqqrtlsm pqllagrket nhaqlelmdg rngtesklnk aygppkpprt
      181 gaalearttn sdrnfdksei rtvlqnwqmg llmnddkald krqlpavnyr sdartllrpt
      241 rilgdgkavd lqnnqvglqp kkplnrrrep rrhtlqngid ynmmkrmkqi eqekdvllqg
      301 ltavdkarew ylkqisatqe kikylgrmgs hveqwteaqq erlelqrarv levnrhlaal
      361 isswergglp lhmnlaflst ptstqlqqdm lsrlkqqnhr lteevskksq riaileqekd
      421 nlvrelynrq sgmiqsrrat miheqhdqtf mlcl
//
LOCUS       XP_034184041             452 aa            linear   INV 03-SEP-2025
DEFINITION  suppressor APC domain-containing protein 2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184041
VERSION     XP_034184041.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328150.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="suppressor APC domain-containing protein 2
                     isoform X2"
                     /calculated_mol_wt=51488
     Region          280..361
                     /region_name="Suppressor_APC"
                     /note="Adenomatous polyposis coli tumour suppressor
                     protein; pfam11414"
                     /db_xref="CDD:463275"
     CDS             1..452
                     /gene="LOC117606109"
                     /coded_by="XM_034328150.2:406..1764"
                     /db_xref="GeneID:117606109"
ORIGIN      
        1 maqiqnsgad glpkhfvsam rtlfdimddq ntgfvkfsdi egrwqddgtq glpkgvldsl
       61 kkvtppngll sferfctglk icllqiqaes dskkhdgqqn rppsapilip dsqnkttwts
      121 pntatirpnn aisqqrtlsm pqllagrket nhaqlelmdg rngtesklnk aygppkpprt
      181 gaalearttn sdrnfdksei rtvlqnwqmg llmnddkald krqlpavnyr sdartllrpt
      241 rilgdgkavd lqnnqvglqp kkplnrrrep rrhtlqngid ynmmkrmkqi eqekdvllqg
      301 ltavdkarew ylkqisatqe kikylgrmgs hveqwteaqq erlelqrarv levnrhlaal
      361 isswergglp lhmnlaflst ptstqlqqdm lsrlkqqnhr lteevskksq riaileqekd
      421 nlvrelynrq sgmiqsrrat miheqhdqtf ii
//
LOCUS       XP_034184047             451 aa            linear   INV 03-SEP-2025
DEFINITION  suppressor APC domain-containing protein 2 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184047
VERSION     XP_034184047.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328156.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..451
                     /product="suppressor APC domain-containing protein 2
                     isoform X3"
                     /calculated_mol_wt=51393
     Region          280..361
                     /region_name="Suppressor_APC"
                     /note="Adenomatous polyposis coli tumour suppressor
                     protein; pfam11414"
                     /db_xref="CDD:463275"
     CDS             1..451
                     /gene="LOC117606109"
                     /coded_by="XM_034328156.2:406..1761"
                     /db_xref="GeneID:117606109"
ORIGIN      
        1 maqiqnsgad glpkhfvsam rtlfdimddq ntgfvkfsdi egrwqddgtq glpkgvldsl
       61 kkvtppngll sferfctglk icllqiqaes dskkhdgqqn rppsapilip dsqnkttwts
      121 pntatirpnn aisqqrtlsm pqllagrket nhaqlelmdg rngtesklnk aygppkpprt
      181 gaalearttn sdrnfdksei rtvlqnwqmg llmnddkald krqlpavnyr sdartllrpt
      241 rilgdgkavd lqnnqvglqp kkplnrrrep rrhtlqngid ynmmkrmkqi eqekdvllqg
      301 ltavdkarew ylkqisatqe kikylgrmgs hveqwteaqq erlelqrarv levnrhlaal
      361 isswergglp lhmnlaflst ptstqlqqdm lsrlkqqnhr lteevskksq riaileqekd
      421 nlvrelynrq sgmiqsrrat miheqhdqtf m
//
LOCUS       XP_034184058             451 aa            linear   INV 03-SEP-2025
DEFINITION  suppressor APC domain-containing protein 2 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184058
VERSION     XP_034184058.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328167.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..451
                     /product="suppressor APC domain-containing protein 2
                     isoform X3"
                     /calculated_mol_wt=51393
     Region          280..361
                     /region_name="Suppressor_APC"
                     /note="Adenomatous polyposis coli tumour suppressor
                     protein; pfam11414"
                     /db_xref="CDD:463275"
     CDS             1..451
                     /gene="LOC117606109"
                     /coded_by="XM_034328167.2:406..1761"
                     /db_xref="GeneID:117606109"
ORIGIN      
        1 maqiqnsgad glpkhfvsam rtlfdimddq ntgfvkfsdi egrwqddgtq glpkgvldsl
       61 kkvtppngll sferfctglk icllqiqaes dskkhdgqqn rppsapilip dsqnkttwts
      121 pntatirpnn aisqqrtlsm pqllagrket nhaqlelmdg rngtesklnk aygppkpprt
      181 gaalearttn sdrnfdksei rtvlqnwqmg llmnddkald krqlpavnyr sdartllrpt
      241 rilgdgkavd lqnnqvglqp kkplnrrrep rrhtlqngid ynmmkrmkqi eqekdvllqg
      301 ltavdkarew ylkqisatqe kikylgrmgs hveqwteaqq erlelqrarv levnrhlaal
      361 isswergglp lhmnlaflst ptstqlqqdm lsrlkqqnhr lteevskksq riaileqekd
      421 nlvrelynrq sgmiqsrrat miheqhdqtf m
//
LOCUS       XP_034184072             449 aa            linear   INV 03-SEP-2025
DEFINITION  diphthamide biosynthesis 2 [Osmia lignaria lignaria].
ACCESSION   XP_034184072
VERSION     XP_034184072.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328181.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184072.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="diphthamide biosynthesis 2"
                     /calculated_mol_wt=50912
     Region          9..331
                     /region_name="diphth2_R"
                     /note="diphthamide biosynthesis enzyme Dph1/Dph2 domain;
                     TIGR00322"
                     /db_xref="CDD:273013"
     CDS             1..449
                     /gene="Dph2"
                     /coded_by="XM_034328181.2:157..1506"
                     /db_xref="GeneID:117606124"
ORIGIN      
        1 mndsvdnlye lkkcskwine cnlnkvclqf pdnllpdsve ialhlenhin kkvyilgdtt
       61 cgsccvdeia aehihadsii hfghsclsps irlpvfhvlp kkeidieeli nrfksnfenk
      121 serilffydv vyahkiediy kilspiyknl iftslnctsn veftqtkdss aisilgrsfk
      181 leenykiqdy iafflgndgk tftalavtip akkwyyfeas niieyealnt pwlkrrrfli
      241 eklkdakvvg ivvatlgikd ylkiitmmkd ilkqknkksy ilsvgkinpv klanfpeida
      301 fvaitcpene vfdsreflkp ilipyevela fnssrecytq ycmdfrqilp sglnyvdfka
      361 stnsdislit gelrncdesv pctdkmsala innsagivai gkagaeylyn rswkgveqrl
      421 gkdavhsaev grcglpssye nepisvekk
//
LOCUS       XP_034190656             429 aa            linear   INV 03-SEP-2025
DEFINITION  tektin-2 [Osmia lignaria lignaria].
ACCESSION   XP_034190656
VERSION     XP_034190656.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334765.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..429
                     /product="tektin-2"
                     /calculated_mol_wt=50091
     Region          20..401
                     /region_name="Tektin"
                     /note="Tektin family; pfam03148"
                     /db_xref="CDD:460827"
     CDS             1..429
                     /gene="LOC117608963"
                     /coded_by="XM_034334765.2:89..1378"
                     /db_xref="GeneID:117608963"
ORIGIN      
        1 maksasiyek plphigladw yakqwelqqn aaarsteafe lrnagqivra etmvktiwdt
       61 ymnnvrladr vtelsrwgev fglllhqlvs eihflkdeka daekeleiln dplhvtaeci
      121 smrdcrrgte ltydeadtel kkelcvienv kklltdrvqa aweklnrlee vrfqiqleve
      181 dkdetikidk enlnldrtca nisykpnalk ipkdsityda wlehcqsikt ladnelsdvy
      241 dfresmnvmr erarndikaq qdvtdfslrk riyqtqkarn elewqklkiq kemeilqkei
      301 vrledallhk idsikcaqtr lenrtyrpgf elcrdeadhg lrnealhlre teqtlkktle
      361 nskaaynnle slltlidrnl ddkqhslatd vmcldmratl ktgdrtrlpn etdrnivltr
      421 mekeiples
//
LOCUS       XP_034184080             341 aa            linear   INV 03-SEP-2025
DEFINITION  ataxin-3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184080
VERSION     XP_034184080.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328189.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="ataxin-3 isoform X1"
                     /calculated_mol_wt=38658
     Region          9..165
                     /region_name="Josephin"
                     /note="pfam02099"
                     /db_xref="CDD:460444"
     CDS             1..341
                     /gene="LOC117606131"
                     /coded_by="XM_034328189.2:265..1290"
                     /db_xref="GeneID:117606131"
ORIGIN      
        1 mdtifhekqe gflcaqhcln allqgpyfna vdlanfghqm deeerirmae sgvdsedykl
       61 fleqpsgnmd dsgyfsvqvi ssalkvwgle lipynstepa aimaqndpiq mkayicnykg
      121 hwftirkigk qwfnlnsvls gpelisdtyl smylaqllqe gysifivigt lpqcpaddil
      181 mrnpiepvsk gqsslkpevk glanytmsnt farrlsskek tttsksqeki isikvekgee
      241 eeddenaelq ralqlsfeen kkndidkylk lsldfhdynk kmcanddtee dddlrralql
      301 slecatappt pesedvhwrq lnhfgtysrt ssgetsqkln i
//
LOCUS       XP_034184084             273 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606131 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184084
VERSION     XP_034184084.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="uncharacterized protein LOC117606131 isoform X2"
                     /calculated_mol_wt=30896
     Region          <1..97
                     /region_name="Josephin"
                     /note="pfam02099"
                     /db_xref="CDD:460444"
     CDS             1..273
                     /gene="LOC117606131"
                     /coded_by="XM_034328193.2:168..989"
                     /db_xref="GeneID:117606131"
ORIGIN      
        1 mddsgyfsvq vissalkvwg lelipynste paaimaqndp iqmkayicny kghwftirki
       61 gkqwfnlnsv lsgpelisdt ylsmylaqll qegysifivi gtlpqcpadd ilmrnpiepv
      121 skgqsslkpe vkglanytms ntfarrlssk ektttsksqe kiisikvekg eeeeddenae
      181 lqralqlsfe enkkndidky lklsldfhdy nkkmcanddt eedddlrral qlslecatap
      241 ptpesedvhw rqlnhfgtys rtssgetsqk lni
//
LOCUS       XP_034190669             332 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608970 [Osmia lignaria lignaria].
ACCESSION   XP_034190669
VERSION     XP_034190669.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334778.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..332
                     /product="uncharacterized protein LOC117608970"
                     /calculated_mol_wt=38737
     Region          121..169
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cd00024"
                     /db_xref="CDD:349274"
     Site            order(121..125,140,142..145,149,151..154,156..157,163,
                     166..167)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     Region          231..281
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(232..236,252,254..257,261,263..266,268..269,275,
                     278..279)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     CDS             1..332
                     /gene="LOC117608970"
                     /coded_by="XM_034334778.2:1..999"
                     /db_xref="GeneID:117608970"
ORIGIN      
        1 mrrlssknkh kksemwsess sdengvartn iidsddkmka gkdnenaemp krgaakkras
       61 rsrhsqkeal enldveaeee dtqsadaeee knvekggask klkkedqksn iikdgnnkde
      121 yevekivgqr tikgrrqfli rwkgygedsd tweqeedlnc sqlieeflae deenheeikp
      181 kksensaksp kkvaevdrks kklknntkrk sendkqitad dgkndkddqk efevekiiev
      241 qykknktref lirwkgftsa ddtwepeenl ncpeliakfm qkvekartme arelranrph
      301 tkrytltthe hgrrlsrrnm dkqratyhec de
//
LOCUS       XP_034190681             240 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab23 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190681
VERSION     XP_034190681.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334790.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="RAS oncogene family member Rab23 isoform X1"
                     /calculated_mol_wt=26734
     Region          10..169
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            16..23
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(18..24,67,121..122,124,151..153)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            41
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            45..47
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            64..67
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(66..67,83..84)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            121..124
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            151..153
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..240
                     /gene="Rab23"
                     /coded_by="XM_034334790.2:318..1040"
                     /db_xref="GeneID:117608976"
ORIGIN      
        1 mreeeleisl kvvivgngav gkssmiqrfc kgtytrdykk tigvdflere ievdgedvrl
       61 mlwdtagqee fdaitaayyr gahacvlays atdrdsfdai pswklkvene cgeiptvlvq
      121 nkmdlvdqcv idpdeaerlg ralgckllrt svkedvgvms vfrhlasrcl hemrrcdddy
      181 qddlrlystg prspsvisaf spngstcsrn tgngtivlrp gaktknhkkr nfvknacill
//
LOCUS       XP_034190688             216 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab23 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190688
VERSION     XP_034190688.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334797.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..216
                     /product="RAS oncogene family member Rab23 isoform X2"
                     /calculated_mol_wt=24207
     Region          1..145
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            17
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            21..23
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            40..43
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(42..43,59..60)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            97..100
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            127..129
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..216
                     /gene="Rab23"
                     /coded_by="XM_034334797.2:568..1218"
                     /db_xref="GeneID:117608976"
ORIGIN      
        1 miqrfckgty trdykktigv dflereievd gedvrlmlwd tagqeefdai taayyrgaha
       61 cvlaysatdr dsfdaipswk lkvenecgei ptvlvqnkmd lvdqcvidpd eaerlgralg
      121 ckllrtsvke dvgvmsvfrh lasrclhemr rcdddyqddl rlystgprsp svisafspng
      181 stcsrntgng tivlrpgakt knhkkrnfvk nacill
//
LOCUS       XP_034190699             185 aa            linear   INV 03-SEP-2025
DEFINITION  BAG family molecular chaperone regulator 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190699
VERSION     XP_034190699.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334808.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..185
                     /product="BAG family molecular chaperone regulator 2"
                     /calculated_mol_wt=21052
     CDS             1..185
                     /gene="LOC117608990"
                     /coded_by="XM_034334808.2:189..746"
                     /db_xref="GeneID:117608990"
ORIGIN      
        1 mdsplphiie sadcdgtskp kdrlvnlldq ievhveqlrk dawrleeerd tllttldtlr
       61 nneilsglee tdkddvlrya erlslrcltv dvlvkiqrdq iqqealhqvn glidsliisl
      121 rddpvgtrqr casfmnacts qslghsdktf etailgctmd dqkrikkrlq glldyidkmh
      181 tiqll
//
LOCUS       XP_034184098             128 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606139 [Osmia lignaria lignaria].
ACCESSION   XP_034184098
VERSION     XP_034184098.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328207.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..128
                     /product="uncharacterized protein LOC117606139"
                     /calculated_mol_wt=14224
     CDS             1..128
                     /gene="LOC117606139"
                     /coded_by="XM_034328207.2:654..1040"
                     /db_xref="GeneID:117606139"
ORIGIN      
        1 mlprlvspii lllviigtls qacdldqmqy gcriynaqcr cgygcsaeyr ynnnedckea
       61 lrgrrgdicy rskpcmnggs clqissepgf kcrcegtgyy gshcdkpcpg pnnnqrfrgp
      121 fpyecvii
//
LOCUS       XP_034190708              75 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L38 [Osmia lignaria lignaria].
ACCESSION   XP_034190708
VERSION     XP_034190708.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334817.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..75
                     /product="ribosomal protein L38"
                     /calculated_mol_wt=8758
     Region          2..68
                     /region_name="Ribosomal_L38e"
                     /note="Ribosomal L38e protein family; pfam01781"
                     /db_xref="CDD:460326"
     CDS             1..75
                     /gene="RpL38"
                     /coded_by="XM_034334817.2:162..389"
                     /db_xref="GeneID:117608999"
ORIGIN      
        1 mpreikeikd fllkarrkda ksvkikknae nvkfkvrcsr flytlvitdk ekaeklkqsl
       61 ppglqvkevk rserm
//
LOCUS       XP_034190717              75 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L38 [Osmia lignaria lignaria].
ACCESSION   XP_034190717
VERSION     XP_034190717.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334826.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..75
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..75
                     /product="ribosomal protein L38"
                     /calculated_mol_wt=8758
     Region          2..68
                     /region_name="Ribosomal_L38e"
                     /note="Ribosomal L38e protein family; pfam01781"
                     /db_xref="CDD:460326"
     CDS             1..75
                     /gene="RpL38"
                     /coded_by="XM_034334826.2:184..411"
                     /db_xref="GeneID:117608999"
ORIGIN      
        1 mpreikeikd fllkarrkda ksvkikknae nvkfkvrcsr flytlvitdk ekaeklkqsl
       61 ppglqvkevk rserm
//
LOCUS       XP_076546187            3135 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546187
VERSION     XP_076546187.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690072.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3135
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X1"
                     /calculated_mol_wt=351714
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2342..2481
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2351,2385..2386,2412,2414,2468,2471..2473,
                     2475..2476,2478..2480)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2677..2766
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2707..2711
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2734..2738
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2748..2753
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2851..3098
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2851..2854,2857,2859,2872,2874,2902,2921..2924,2967,
                     2971..2972,2974,2985..2986)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3135
                     /gene="trio"
                     /coded_by="XM_076690072.1:267..9674"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdg kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf
     2401 tnpayiykah iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt
     2461 itailqtqrd flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern
     2521 emtairaagk afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr
     2581 spvrnnsipi vpgsrvrlta dwgklragee veicrpdgpg vivsrivgkm eefwipaslv
     2641 pnssisraws frprridseg esvhlpqdvh aeengpptil tipcsirata ggvarlvlea
     2701 rrveratvrw rkegqrsnie ptdryrlfrt sdslcleiap clpsdsgiyh crvehetgsc
     2761 sakiplrivg mslflislhi ssrihqtvnf pgintnawcd tiegnrsdes ssnnnsssti
     2821 varsnftsna tkdrkmeser ftdryielee lgngrfakvc rarekgsvre valkqisrvr
     2881 qrvsltraey dflratrhdn ivkalalfed lpqpgvdtiv lelvngptlf ghlgekneyt
     2941 eaivakytgq llsalqwlha rkqahldlkp envlidnetd tvklidlgea vraapldevr
     3001 ppadlefaap esvlgkptgs ytdmwaagvf iyvllsglsp flddsieett anilkcdfcf
     3061 pdeyfqeise qvkrlldkll rlrgedrtta eislssawfq vpigatipss rlaafidrra
     3121 hrsksrqern ssfys
//
LOCUS       XP_076546188            3135 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546188
VERSION     XP_076546188.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690073.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3135
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X1"
                     /calculated_mol_wt=351714
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2342..2481
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2351,2385..2386,2412,2414,2468,2471..2473,
                     2475..2476,2478..2480)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2677..2766
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2707..2711
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2734..2738
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2748..2753
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2851..3098
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2851..2854,2857,2859,2872,2874,2902,2921..2924,2967,
                     2971..2972,2974,2985..2986)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3135
                     /gene="trio"
                     /coded_by="XM_076690073.1:170..9577"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdg kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf
     2401 tnpayiykah iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt
     2461 itailqtqrd flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern
     2521 emtairaagk afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr
     2581 spvrnnsipi vpgsrvrlta dwgklragee veicrpdgpg vivsrivgkm eefwipaslv
     2641 pnssisraws frprridseg esvhlpqdvh aeengpptil tipcsirata ggvarlvlea
     2701 rrveratvrw rkegqrsnie ptdryrlfrt sdslcleiap clpsdsgiyh crvehetgsc
     2761 sakiplrivg mslflislhi ssrihqtvnf pgintnawcd tiegnrsdes ssnnnsssti
     2821 varsnftsna tkdrkmeser ftdryielee lgngrfakvc rarekgsvre valkqisrvr
     2881 qrvsltraey dflratrhdn ivkalalfed lpqpgvdtiv lelvngptlf ghlgekneyt
     2941 eaivakytgq llsalqwlha rkqahldlkp envlidnetd tvklidlgea vraapldevr
     3001 ppadlefaap esvlgkptgs ytdmwaagvf iyvllsglsp flddsieett anilkcdfcf
     3061 pdeyfqeise qvkrlldkll rlrgedrtta eislssawfq vpigatipss rlaafidrra
     3121 hrsksrqern ssfys
//
LOCUS       XP_076546189            3135 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546189
VERSION     XP_076546189.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690074.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3135
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X1"
                     /calculated_mol_wt=351714
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2342..2481
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2351,2385..2386,2412,2414,2468,2471..2473,
                     2475..2476,2478..2480)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2677..2766
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2707..2711
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2734..2738
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2748..2753
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2851..3098
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2851..2854,2857,2859,2872,2874,2902,2921..2924,2967,
                     2971..2972,2974,2985..2986)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3135
                     /gene="trio"
                     /coded_by="XM_076690074.1:182..9589"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdg kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf
     2401 tnpayiykah iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt
     2461 itailqtqrd flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern
     2521 emtairaagk afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr
     2581 spvrnnsipi vpgsrvrlta dwgklragee veicrpdgpg vivsrivgkm eefwipaslv
     2641 pnssisraws frprridseg esvhlpqdvh aeengpptil tipcsirata ggvarlvlea
     2701 rrveratvrw rkegqrsnie ptdryrlfrt sdslcleiap clpsdsgiyh crvehetgsc
     2761 sakiplrivg mslflislhi ssrihqtvnf pgintnawcd tiegnrsdes ssnnnsssti
     2821 varsnftsna tkdrkmeser ftdryielee lgngrfakvc rarekgsvre valkqisrvr
     2881 qrvsltraey dflratrhdn ivkalalfed lpqpgvdtiv lelvngptlf ghlgekneyt
     2941 eaivakytgq llsalqwlha rkqahldlkp envlidnetd tvklidlgea vraapldevr
     3001 ppadlefaap esvlgkptgs ytdmwaagvf iyvllsglsp flddsieett anilkcdfcf
     3061 pdeyfqeise qvkrlldkll rlrgedrtta eislssawfq vpigatipss rlaafidrra
     3121 hrsksrqern ssfys
//
LOCUS       XP_034174587            3113 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174587
VERSION     XP_034174587.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318696.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174587.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3113
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X2"
                     /calculated_mol_wt=349234
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2342..2481
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2351,2385..2386,2412,2414,2468,2471..2473,
                     2475..2476,2478..2480)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2677..2766
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2707..2711
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2734..2738
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2748..2753
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2829..3076
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2829..2832,2835,2837,2850,2852,2880,2899..2902,2945,
                     2949..2950,2952,2963..2964)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3113
                     /gene="trio"
                     /coded_by="XM_034318696.2:267..9608"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdg kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf
     2401 tnpayiykah iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt
     2461 itailqtqrd flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern
     2521 emtairaagk afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr
     2581 spvrnnsipi vpgsrvrlta dwgklragee veicrpdgpg vivsrivgkm eefwipaslv
     2641 pnssisraws frprridseg esvhlpqdvh aeengpptil tipcsirata ggvarlvlea
     2701 rrveratvrw rkegqrsnie ptdryrlfrt sdslcleiap clpsdsgiyh crvehetgsc
     2761 sakiplrivg intnawcdti egnrsdesss nnnssstiva rsnftsnatk drkmeserft
     2821 dryieleelg ngrfakvcra rekgsvreva lkqisrvrqr vsltraeydf lratrhdniv
     2881 kalalfedlp qpgvdtivle lvngptlfgh lgekneytea ivakytgqll salqwlhark
     2941 qahldlkpen vlidnetdtv klidlgeavr aapldevrpp adlefaapes vlgkptgsyt
     3001 dmwaagvfiy vllsglspfl ddsieettan ilkcdfcfpd eyfqeiseqv krlldkllrl
     3061 rgedrttaei slssawfqvp igatipssrl aafidrrahr sksrqernss fys
//
LOCUS       XP_076546190            3103 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546190
VERSION     XP_076546190.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690075.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3103
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X3"
                     /calculated_mol_wt=348007
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          550..755
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          770..978
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            876..881
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          878..1101
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1106..1206
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1208..1213
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1255..1425
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1259,1263,1358,1379..1380,1383..1384,1386..1387,
                     1390..1391,1394..1395,1398,1421,1425)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1432..1554
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1449,1489,1491)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1612..1681
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1618,1620,1623,1628,1653..1654,1675,1677..1678)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2128..2305
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2310..2449
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2319,2353..2354,2380,2382,2436,2439..2441,
                     2443..2444,2446..2448)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2645..2734
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2675..2679
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2702..2706
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2716..2721
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2819..3066
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2819..2822,2825,2827,2840,2842,2870,2889..2892,2935,
                     2939..2940,2942,2953..2954)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3103
                     /gene="trio"
                     /coded_by="XM_076690075.1:267..9578"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy sqdghggidg htgmsgnpaa dysegashvl avihqilghh ralearwhar
      541 kvklhqrlal rlfqedvkqv ldwltnhgev firkntgvgr nlqkarvyqk shehfenvaq
      601 ntytnatkll taaqelahtg ecaadeiyav aqeleahvss faarveqrrr rldlavvfyt
      661 hekelsgwvd elrqelqqde vaenletaer lleqcaqhra scleacasti vqgeallrel
      721 restdapdtt gsiaaveaal drlaglrqel eelwatrklr lelclrlrvf erdaleasgq
      781 lemwaqelqg ppregspeql lrvhndgvah mqntafqvlq qgqelaqvle qagvcimadg
      841 qhsaaarvqv lleflnerem daedlaemrr vrleqasqlv qlqtdathva swirngeaml
      901 laslrvpenl qdaeqlrleh eqfqvaiekt htsavqvkhr adalvsanhy dpksirevae
      961 dvtkrwqqlv tcaeerhklv tasinfykta eqvrsvldsl ereykrdedw casgdkagqv
     1021 ptlvgkhqeq keaflkactl vrrtaetflk ytnrslqfys yqansagsen kvksileell
     1081 skenrvleyw tqrkkrldqc hqyvlfersa kqalewiret gelylathtn vgknrieneq
     1141 llrehnefkg aaketrervk lliqladnlv ekghahaaai kqsvaevdqr ykcfssrmdy
     1201 yktqieddlg iqsddgqkdl sidrnsdpll eekikgkdlk elneekrrsa rrkefimael
     1261 lqtertyvkd letcircfle etrsgkgnip sglqgresii fsnmeeihqf hsniflrele
     1321 kyetmpedvg hcfvtwapkf dmyvmycknk pesnqflvth ggtwfeelqr khrvehpiaa
     1381 ylikpvqrit kyqlllkdlq accqegqgei kdglevmlnv pkkandalhl smlegcdvri
     1441 dtlgdvvlqd sftvwdpkql irkgrdrhif lfefhllfsk evkdsagkvk yiyksrlmts
     1501 elgvtehieg deckfavwtg raptsdtrvv lransmdakq lwvkrlrevi qetyfslsmp
     1561 kspakksssq rssrdleeca slddsvenld rnslasfgst nttdsdktgv aevtwviadh
     1621 laapgsrelt vtkgqqvevl engsnisgvn taewthvrll vapgqvdppp eglvptsalk
     1681 qpppvsnkts psrkapaqqq qqqqqqqqqq qqqqqqlssn qiqstspsgi vtavvssati
     1741 gtggssscss sssssvitgv pstgytvhnv qnvvapllpd eaenviasdg stgtntsspg
     1801 nkrrgfsgrk wlppplrkls qgkvekssgt titatpgsag isipatvtta ttttiatptp
     1861 pspppppppl tstatdrssl kknvsekrfk lpsgadqtra srassvsist ltsaaasmrv
     1921 sgsvtdvdld ttelvrttie aeteeeegei erseaeleed ttepddaetl tyseqngadd
     1981 aedelelppp mkpitepilv gtsngssnst istetggksr tserstkild gattadlaei
     2041 eqivkermeq htenlerqsl mrtpsgksss nvgsggedtr eeedslpttm siasitspss
     2101 iattsspaht npdehdpest alakrqfvir elvetekdyv ndlkqivegy mafmrdpece
     2161 islpddlrgg kdkmvfgnie aiyewhrdff lkaldrcler peelgplfkr yerklhmyvv
     2221 ycqnkpvsey ivseyidtyf eelrqklghr lqlcdllikp vqritkyqll lrealrlter
     2281 tqrvaeiegl raavhvmrvi pkaandmmdv grlqgfdgki taqgklllhg pllvtelsnl
     2341 pskgrewqvf lfeqniifse avgkktqftn payiykahiq vnkmsleecy edpekfvirs
     2401 tdprkpslgf sciaieengp rkqdwvdtit ailqtqrdfl kaiqspiayq keltkdpfrg
     2461 vspespcrgg vlstippnls ntseeernem tairaagkaf aatttsitti sqqsrtgars
     2521 dqdslqtpsp sksrlnfleg fksslrprsp vrnnsipivp gsrvrltadw gklrageeve
     2581 icrpdgpgvi vsrivgkmee fwipaslvpn ssisrawsfr prridseges vhlpqdvhae
     2641 engpptilti pcsiratagg varlvlearr veratvrwrk egqrsniept dryrlfrtsd
     2701 slcleiapcl psdsgiyhcr vehetgscsa kiplrivgms lflislhiss rihqtvnfpg
     2761 intnawcdti egnrsdesss nnnssstiva rsnftsnatk drkmeserft dryieleelg
     2821 ngrfakvcra rekgsvreva lkqisrvrqr vsltraeydf lratrhdniv kalalfedlp
     2881 qpgvdtivle lvngptlfgh lgekneytea ivakytgqll salqwlhark qahldlkpen
     2941 vlidnetdtv klidlgeavr aapldevrpp adlefaapes vlgkptgsyt dmwaagvfiy
     3001 vllsglspfl ddsieettan ilkcdfcfpd eyfqeiseqv krlldkllrl rgedrttaei
     3061 slssawfqvp igatipssrl aafidrrahr sksrqernss fys
//
LOCUS       XP_076546191            3097 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546191
VERSION     XP_076546191.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690076.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3097
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X4"
                     /calculated_mol_wt=347560
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          544..749
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          764..972
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            870..875
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          872..1095
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1100..1200
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1202..1207
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1249..1419
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1253,1257,1352,1373..1374,1377..1378,1380..1381,
                     1384..1385,1388..1389,1392,1415,1419)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1426..1548
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1443,1483,1485)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1606..1675
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1612,1614,1617,1622,1647..1648,1669,1671..1672)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2122..2299
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2304..2443
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2313,2347..2348,2374,2376,2430,2433..2435,
                     2437..2438,2440..2442)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2639..2728
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2669..2673
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2696..2700
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2710..2715
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2813..3060
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2813..2816,2819,2821,2834,2836,2864,2883..2886,2929,
                     2933..2934,2936,2947..2948)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3097
                     /gene="trio"
                     /coded_by="XM_076690076.1:267..9560"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevhstskkl lyqldhlvqv cnqpqgidht
      481 trkhsqdghg gidghtgmsg npaadysega shvlavihqi lghhralear wharkvklhq
      541 rlalrlfqed vkqvldwltn hgevfirknt gvgrnlqkar vyqkshehfe nvaqntytna
      601 tklltaaqel ahtgecaade iyavaqelea hvssfaarve qrrrrldlav vfythekels
      661 gwvdelrqel qqdevaenle taerlleqca qhrascleac astivqgeal lrelrestda
      721 pdttgsiaav eaaldrlagl rqeleelwat rklrlelclr lrvferdale asgqlemwaq
      781 elqgppregs peqllrvhnd gvahmqntaf qvlqqgqela qvleqagvci madgqhsaaa
      841 rvqvllefln eremdaedla emrrvrleqa sqlvqlqtda thvaswirng eamllaslrv
      901 penlqdaeql rleheqfqva iekthtsavq vkhradalvs anhydpksir evaedvtkrw
      961 qqlvtcaeer hklvtasinf yktaeqvrsv ldslereykr dedwcasgdk agqvptlvgk
     1021 hqeqkeaflk actlvrrtae tflkytnrsl qfysyqansa gsenkvksil eellskenrv
     1081 leywtqrkkr ldqchqyvlf ersakqalew iretgelyla thtnvgknri eneqllrehn
     1141 efkgaaketr ervklliqla dnlvekghah aaaikqsvae vdqrykcfss rmdyyktqie
     1201 ddlgiqsddg qkdlsidrns dplleekikg kdlkelneek rrsarrkefi maellqtert
     1261 yvkdletcir cfleetrsgk gnipsglqgr esiifsnmee ihqfhsnifl relekyetmp
     1321 edvghcfvtw apkfdmyvmy cknkpesnqf lvthggtwfe elqrkhrveh piaaylikpv
     1381 qritkyqlll kdlqaccqeg qgeikdglev mlnvpkkand alhlsmlegc dvridtlgdv
     1441 vlqdsftvwd pkqlirkgrd rhiflfefhl lfskevkdsa gkvkyiyksr lmtselgvte
     1501 hiegdeckfa vwtgraptsd trvvlransm dakqlwvkrl reviqetyfs lsmpkspakk
     1561 sssqrssrdl eecaslddsv enldrnslas fgstnttdsd ktgvaevtwv iadhlaapgs
     1621 reltvtkgqq vevlengsni sgvntaewth vrllvapgqv dpppeglvpt salkqpppvs
     1681 nktspsrkap aqqqqqqqqq qqqqqqqqqq lssnqiqsts psgivtavvs satigtggss
     1741 scsssssssv itgvpstgyt vhnvqnvvap llpdeaenvi asdgstgtnt sspgnkrrgf
     1801 sgrkwlpppl rklsqgkvek ssgttitatp gsagisipat vttattttia tptppspppp
     1861 pppltstatd rsslkknvse krfklpsgad qtrasrassv sistltsaaa smrvsgsvtd
     1921 vdldttelvr ttieaeteee egeierseae leedttepdd aetltyseqn gaddaedele
     1981 lpppmkpite pilvgtsngs snstistetg gksrtserst kildgattad laeieqivke
     2041 rmeqhtenle rqslmrtpsg ksssnvgsgg edtreeedsl pttmsiasit spssiattss
     2101 pahtnpdehd pestalakrq fvirelvete kdyvndlkqi vegymafmrd peceislpdd
     2161 lrggkdkmvf gnieaiyewh rdfflkaldr clerpeelgp lfkryerklh myvvycqnkp
     2221 vseyivseyi dtyfeelrqk lghrlqlcdl likpvqritk yqlllrealr ltertqrvae
     2281 ieglraavhv mrvipkaand mmdvgrlqgf dgkitaqgkl llhgpllvte lsnlpskgre
     2341 wqvflfeqni ifseavgkkt qftnpayiyk ahiqvnkmsl eecyedpekf virstdprkp
     2401 slgfsciaie engprkqdwv dtitailqtq rdflkaiqsp iayqkeltkd pfrgvspesp
     2461 crggvlstip pnlsntseee rnemtairaa gkafaattts ittisqqsrt garsdqdslq
     2521 tpspsksrln flegfksslr prspvrnnsi pivpgsrvrl tadwgklrag eeveicrpdg
     2581 pgvivsrivg kmeefwipas lvpnssisra wsfrprrids egesvhlpqd vhaeengppt
     2641 iltipcsira taggvarlvl earrveratv rwrkegqrsn ieptdryrlf rtsdslclei
     2701 apclpsdsgi yhcrvehetg scsakiplri vgmslflisl hissrihqtv nfpgintnaw
     2761 cdtiegnrsd esssnnnsss tivarsnfts natkdrkmes erftdryiel eelgngrfak
     2821 vcrarekgsv revalkqisr vrqrvsltra eydflratrh dnivkalalf edlpqpgvdt
     2881 ivlelvngpt lfghlgekne yteaivakyt gqllsalqwl harkqahldl kpenvlidne
     2941 tdtvklidlg eavraaplde vrppadlefa apesvlgkpt gsytdmwaag vfiyvllsgl
     3001 spflddsiee ttanilkcdf cfpdeyfqei seqvkrlldk llrlrgedrt taeislssaw
     3061 fqvpigatip ssrlaafidr rahrsksrqe rnssfys
//
LOCUS       XP_034174622            3081 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174622
VERSION     XP_034174622.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318731.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174622.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3081
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X5"
                     /calculated_mol_wt=345527
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          550..755
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          770..978
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            876..881
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          878..1101
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1106..1206
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1208..1213
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1255..1425
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1259,1263,1358,1379..1380,1383..1384,1386..1387,
                     1390..1391,1394..1395,1398,1421,1425)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1432..1554
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1449,1489,1491)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1612..1681
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1618,1620,1623,1628,1653..1654,1675,1677..1678)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2128..2305
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2310..2449
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2319,2353..2354,2380,2382,2436,2439..2441,
                     2443..2444,2446..2448)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2645..2734
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2675..2679
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2702..2706
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2716..2721
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2797..3044
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2797..2800,2803,2805,2818,2820,2848,2867..2870,2913,
                     2917..2918,2920,2931..2932)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3081
                     /gene="trio"
                     /coded_by="XM_034318731.2:267..9512"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy sqdghggidg htgmsgnpaa dysegashvl avihqilghh ralearwhar
      541 kvklhqrlal rlfqedvkqv ldwltnhgev firkntgvgr nlqkarvyqk shehfenvaq
      601 ntytnatkll taaqelahtg ecaadeiyav aqeleahvss faarveqrrr rldlavvfyt
      661 hekelsgwvd elrqelqqde vaenletaer lleqcaqhra scleacasti vqgeallrel
      721 restdapdtt gsiaaveaal drlaglrqel eelwatrklr lelclrlrvf erdaleasgq
      781 lemwaqelqg ppregspeql lrvhndgvah mqntafqvlq qgqelaqvle qagvcimadg
      841 qhsaaarvqv lleflnerem daedlaemrr vrleqasqlv qlqtdathva swirngeaml
      901 laslrvpenl qdaeqlrleh eqfqvaiekt htsavqvkhr adalvsanhy dpksirevae
      961 dvtkrwqqlv tcaeerhklv tasinfykta eqvrsvldsl ereykrdedw casgdkagqv
     1021 ptlvgkhqeq keaflkactl vrrtaetflk ytnrslqfys yqansagsen kvksileell
     1081 skenrvleyw tqrkkrldqc hqyvlfersa kqalewiret gelylathtn vgknrieneq
     1141 llrehnefkg aaketrervk lliqladnlv ekghahaaai kqsvaevdqr ykcfssrmdy
     1201 yktqieddlg iqsddgqkdl sidrnsdpll eekikgkdlk elneekrrsa rrkefimael
     1261 lqtertyvkd letcircfle etrsgkgnip sglqgresii fsnmeeihqf hsniflrele
     1321 kyetmpedvg hcfvtwapkf dmyvmycknk pesnqflvth ggtwfeelqr khrvehpiaa
     1381 ylikpvqrit kyqlllkdlq accqegqgei kdglevmlnv pkkandalhl smlegcdvri
     1441 dtlgdvvlqd sftvwdpkql irkgrdrhif lfefhllfsk evkdsagkvk yiyksrlmts
     1501 elgvtehieg deckfavwtg raptsdtrvv lransmdakq lwvkrlrevi qetyfslsmp
     1561 kspakksssq rssrdleeca slddsvenld rnslasfgst nttdsdktgv aevtwviadh
     1621 laapgsrelt vtkgqqvevl engsnisgvn taewthvrll vapgqvdppp eglvptsalk
     1681 qpppvsnkts psrkapaqqq qqqqqqqqqq qqqqqqlssn qiqstspsgi vtavvssati
     1741 gtggssscss sssssvitgv pstgytvhnv qnvvapllpd eaenviasdg stgtntsspg
     1801 nkrrgfsgrk wlppplrkls qgkvekssgt titatpgsag isipatvtta ttttiatptp
     1861 pspppppppl tstatdrssl kknvsekrfk lpsgadqtra srassvsist ltsaaasmrv
     1921 sgsvtdvdld ttelvrttie aeteeeegei erseaeleed ttepddaetl tyseqngadd
     1981 aedelelppp mkpitepilv gtsngssnst istetggksr tserstkild gattadlaei
     2041 eqivkermeq htenlerqsl mrtpsgksss nvgsggedtr eeedslpttm siasitspss
     2101 iattsspaht npdehdpest alakrqfvir elvetekdyv ndlkqivegy mafmrdpece
     2161 islpddlrgg kdkmvfgnie aiyewhrdff lkaldrcler peelgplfkr yerklhmyvv
     2221 ycqnkpvsey ivseyidtyf eelrqklghr lqlcdllikp vqritkyqll lrealrlter
     2281 tqrvaeiegl raavhvmrvi pkaandmmdv grlqgfdgki taqgklllhg pllvtelsnl
     2341 pskgrewqvf lfeqniifse avgkktqftn payiykahiq vnkmsleecy edpekfvirs
     2401 tdprkpslgf sciaieengp rkqdwvdtit ailqtqrdfl kaiqspiayq keltkdpfrg
     2461 vspespcrgg vlstippnls ntseeernem tairaagkaf aatttsitti sqqsrtgars
     2521 dqdslqtpsp sksrlnfleg fksslrprsp vrnnsipivp gsrvrltadw gklrageeve
     2581 icrpdgpgvi vsrivgkmee fwipaslvpn ssisrawsfr prridseges vhlpqdvhae
     2641 engpptilti pcsiratagg varlvlearr veratvrwrk egqrsniept dryrlfrtsd
     2701 slcleiapcl psdsgiyhcr vehetgscsa kiplrivgin tnawcdtieg nrsdesssnn
     2761 nssstivars nftsnatkdr kmeserftdr yieleelgng rfakvcrare kgsvrevalk
     2821 qisrvrqrvs ltraeydflr atrhdnivka lalfedlpqp gvdtivlelv ngptlfghlg
     2881 ekneyteaiv akytgqllsa lqwlharkqa hldlkpenvl idnetdtvkl idlgeavraa
     2941 pldevrppad lefaapesvl gkptgsytdm waagvfiyvl lsglspfldd sieettanil
     3001 kcdfcfpdey fqeiseqvkr lldkllrlrg edrttaeisl ssawfqvpig atipssrlaa
     3061 fidrrahrsk srqernssfy s
//
LOCUS       XP_076546192            3072 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546192
VERSION     XP_076546192.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690077.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3072
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X6"
                     /calculated_mol_wt=344619
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          <2350..2418
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          2614..2703
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2644..2648
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2671..2675
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2685..2690
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2788..3035
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2788..2791,2794,2796,2809,2811,2839,2858..2861,2904,
                     2908..2909,2911,2922..2923)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3072
                     /gene="trio"
                     /coded_by="XM_076690077.1:269..9487"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdv nkmsleecye dpekfvirst dprkpslgfs ciaieengpr kqdwvdtita
     2401 ilqtqrdflk aiqspiayqk eltkdpfrgv spespcrggv lstippnlsn tseeernemt
     2461 airaagkafa atttsittis qqsrtgarsd qdslqtpsps ksrlnflegf ksslrprspv
     2521 rnnsipivpg srvrltadwg klrageevei crpdgpgviv srivgkmeef wipaslvpns
     2581 sisrawsfrp rridsegesv hlpqdvhaee ngpptiltip csirataggv arlvlearrv
     2641 eratvrwrke gqrsnieptd ryrlfrtsds lcleiapclp sdsgiyhcrv ehetgscsak
     2701 iplrivgmsl flislhissr ihqtvnfpgi ntnawcdtie gnrsdesssn nnssstivar
     2761 snftsnatkd rkmeserftd ryieleelgn grfakvcrar ekgsvreval kqisrvrqrv
     2821 sltraeydfl ratrhdnivk alalfedlpq pgvdtivlel vngptlfghl gekneyteai
     2881 vakytgqlls alqwlharkq ahldlkpenv lidnetdtvk lidlgeavra apldevrppa
     2941 dlefaapesv lgkptgsytd mwaagvfiyv llsglspfld dsieettani lkcdfcfpde
     3001 yfqeiseqvk rlldkllrlr gedrttaeis lssawfqvpi gatipssrla afidrrahrs
     3061 ksrqernssf ys
//
LOCUS       XP_076546193            3065 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546193
VERSION     XP_076546193.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690078.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3065
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X7"
                     /calculated_mol_wt=343853
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          294..510
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            399..404
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          512..717
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          732..940
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            838..843
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          840..1063
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1068..1168
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1170..1175
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1217..1387
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1221,1225,1320,1341..1342,1345..1346,1348..1349,
                     1352..1353,1356..1357,1360,1383,1387)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1394..1516
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1411,1451,1453)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1574..1643
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1580,1582,1585,1590,1615..1616,1637,1639..1640)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2090..2267
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2272..2411
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2281,2315..2316,2342,2344,2398,2401..2403,
                     2405..2406,2408..2410)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2607..2696
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2637..2641
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2664..2668
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2678..2683
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          2781..3028
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(2781..2784,2787,2789,2802,2804,2832,2851..2854,2897,
                     2901..2902,2904,2915..2916)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..3065
                     /gene="trio"
                     /coded_by="XM_076690078.1:267..9464"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tesqdghggi dghtgmsgnp aadysegash
      481 vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg evfirkntgv
      541 grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy avaqeleahv
      601 ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta erlleqcaqh
      661 rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq eleelwatrk
      721 lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv ahmqntafqv
      781 lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem rrvrleqasq
      841 lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie kthtsavqvk
      901 hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk taeqvrsvld
      961 slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf lkytnrslqf
     1021 ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer sakqalewir
     1081 etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn lvekghahaa
     1141 aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp lleekikgkd
     1201 lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn ipsglqgres
     1261 iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck nkpesnqflv
     1321 thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg eikdglevml
     1381 nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh iflfefhllf
     1441 skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr vvlransmda
     1501 kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven ldrnslasfg
     1561 stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg vntaewthvr
     1621 llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq qqqqqqqqls
     1681 snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh nvqnvvapll
     1741 pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss gttitatpgs
     1801 agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr fklpsgadqt
     1861 rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg eierseaele
     1921 edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn stistetggk
     1981 srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks ssnvgsgged
     2041 treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv irelvetekd
     2101 yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd fflkaldrcl
     2161 erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg hrlqlcdlli
     2221 kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm dvgrlqgfdg
     2281 kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf tnpayiykah
     2341 iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt itailqtqrd
     2401 flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern emtairaagk
     2461 afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr spvrnnsipi
     2521 vpgsrvrlta dwgklragee veicrpdgpg vivsrivgkm eefwipaslv pnssisraws
     2581 frprridseg esvhlpqdvh aeengpptil tipcsirata ggvarlvlea rrveratvrw
     2641 rkegqrsnie ptdryrlfrt sdslcleiap clpsdsgiyh crvehetgsc sakiplrivg
     2701 mslflislhi ssrihqtvnf pgintnawcd tiegnrsdes ssnnnsssti varsnftsna
     2761 tkdrkmeser ftdryielee lgngrfakvc rarekgsvre valkqisrvr qrvsltraey
     2821 dflratrhdn ivkalalfed lpqpgvdtiv lelvngptlf ghlgekneyt eaivakytgq
     2881 llsalqwlha rkqahldlkp envlidnetd tvklidlgea vraapldevr ppadlefaap
     2941 esvlgkptgs ytdmwaagvf iyvllsglsp flddsieett anilkcdfcf pdeyfqeise
     3001 qvkrlldkll rlrgedrtta eislssawfq vpigatipss rlaafidrra hrsksrqern
     3061 ssfys
//
LOCUS       XP_034174641            2822 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174641
VERSION     XP_034174641.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318750.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174641.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2822
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X8"
                     /calculated_mol_wt=316738
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2342..2481
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2351,2385..2386,2412,2414,2468,2471..2473,
                     2475..2476,2478..2480)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     Region          2677..2766
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          2707..2711
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          2734..2738
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          2748..2753
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..2822
                     /gene="trio"
                     /coded_by="XM_034318750.2:269..8737"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdg kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf
     2401 tnpayiykah iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt
     2461 itailqtqrd flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern
     2521 emtairaagk afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr
     2581 spvrnnsipi vpgsrvrlta dwgklragee veicrpdgpg vivsrivgkm eefwipaslv
     2641 pnssisraws frprridseg esvhlpqdvh aeengpptil tipcsirata ggvarlvlea
     2701 rrveratvrw rkegqrsnie ptdryrlfrt sdslcleiap clpsdsgiyh crvehetgsc
     2761 sakiplrivs irtlgairsk vidrtnqavt tthlrrslrv pisqamrrki erwkvnvlpt
     2821 dt
//
LOCUS       XP_034174651            2619 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X9 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174651
VERSION     XP_034174651.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174651.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2619
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X9"
                     /calculated_mol_wt=293687
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2342..2481
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2351,2385..2386,2412,2414,2468,2471..2473,
                     2475..2476,2478..2480)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     CDS             1..2619
                     /gene="trio"
                     /coded_by="XM_034318760.2:268..8127"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdg kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf
     2401 tnpayiykah iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt
     2461 itailqtqrd flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern
     2521 emtairaagk afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr
     2581 spvrnnsipg dgrrsgstra ggttrrtcnr pveeggtti
//
LOCUS       XP_034174661            2599 aa            linear   INV 03-SEP-2025
DEFINITION  trio Rho guanine nucleotide exchange factor isoform X10 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174661
VERSION     XP_034174661.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174661.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2599
                     /product="trio Rho guanine nucleotide exchange factor
                     isoform X10"
                     /calculated_mol_wt=291594
     Region          20..152
                     /region_name="SEC14"
                     /note="Domain in homologues of a S. cerevisiae
                     phosphatidylinositol transfer protein (Sec14p);
                     smart00516"
                     /db_xref="CDD:214706"
     Region          166..392
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            292..297
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          582..787
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          802..1010
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            908..913
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          910..1133
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Region          1138..1238
                     /region_name="SPEC"
                     /note="Spectrin repeats; smart00150"
                     /db_xref="CDD:197544"
     Site            1240..1245
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1287..1457
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1291,1295,1390,1411..1412,1415..1416,1418..1419,
                     1422..1423,1426..1427,1430,1453,1457)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1464..1586
                     /region_name="PH1_Kalirin_Trio_like"
                     /note="Triple functional domain pleckstrin homology
                     pleckstrin homology (PH) domain, repeat 1; cd13240"
                     /db_xref="CDD:270060"
     Site            order(1481,1521,1523)
                     /site_type="other"
                     /note="Rac1 binding site [polypeptide binding]"
                     /db_xref="CDD:270060"
     Region          1644..1713
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(1650,1652,1655,1660,1685..1686,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212786"
     Region          2160..2337
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          2342..2481
                     /region_name="PH2_Kalirin_Trio_p63RhoGEF"
                     /note="p63RhoGEF pleckstrin homology (PH) domain, repeat
                     2; cd13241"
                     /db_xref="CDD:270061"
     Site            order(2351,2385..2386,2412,2414,2468,2471..2473,
                     2475..2476,2478..2480)
                     /site_type="other"
                     /note="Galpha i/q binding site [polypeptide binding]"
                     /db_xref="CDD:270061"
     CDS             1..2599
                     /gene="trio"
                     /coded_by="XM_034318770.2:268..8067"
                     /db_xref="GeneID:117601649"
ORIGIN      
        1 mdgtraaevl pllqdrlail pggrdrrggp vlvfpttprr ertkpedyrr llqylltvpg
       61 dearglrftv ivdmrgatwd svkpilkvlh ehfhrsvhva fiikpenfwq kqrtslakqk
      121 kynfeintis lealtkvidp sqltadldgs lqydhaqwid trlavedftw qagelldrld
      181 dlqedlsrnd faddasgakh gidlhnemkk kimkvpveei qvigqrllqr fnngisgtgs
      241 eggsiesgaa ggtdpdgral aalviqhlds lhaaqqhllq lwhikkmkld qcfqlrlfeq
      301 dcekmfdwic hnregflany veigrsyqla kslqeehkhf tmssmnvyvn inqiltasrl
      361 letqhyaagh vravanrldr awkefaagld ertavlglsv vfhhkaeqyv dnvagwsqac
      421 dvgnlpneip ileshirqhq slyeamcqay tevydayqal lsglgsmlqv chsfgsthgt
      481 dsdtfriddy vhstskklly qldhlvqvcn qpqgidhttr khsqdghggi dghtgmsgnp
      541 aadysegash vlavihqilg hhralearwh arkvklhqrl alrlfqedvk qvldwltnhg
      601 evfirkntgv grnlqkarvy qkshehfenv aqntytnatk lltaaqelah tgecaadeiy
      661 avaqeleahv ssfaarveqr rrrldlavvf ythekelsgw vdelrqelqq devaenleta
      721 erlleqcaqh rascleacas tivqgeallr elrestdapd ttgsiaavea aldrlaglrq
      781 eleelwatrk lrlelclrlr vferdaleas gqlemwaqel qgppregspe qllrvhndgv
      841 ahmqntafqv lqqgqelaqv leqagvcima dgqhsaaarv qvlleflner emdaedlaem
      901 rrvrleqasq lvqlqtdath vaswirngea mllaslrvpe nlqdaeqlrl eheqfqvaie
      961 kthtsavqvk hradalvsan hydpksirev aedvtkrwqq lvtcaeerhk lvtasinfyk
     1021 taeqvrsvld slereykrde dwcasgdkag qvptlvgkhq eqkeaflkac tlvrrtaetf
     1081 lkytnrslqf ysyqansags enkvksilee llskenrvle ywtqrkkrld qchqyvlfer
     1141 sakqalewir etgelylath tnvgknrien eqllrehnef kgaaketrer vklliqladn
     1201 lvekghahaa aikqsvaevd qrykcfssrm dyyktqiedd lgiqsddgqk dlsidrnsdp
     1261 lleekikgkd lkelneekrr sarrkefima ellqtertyv kdletcircf leetrsgkgn
     1321 ipsglqgres iifsnmeeih qfhsniflre lekyetmped vghcfvtwap kfdmyvmyck
     1381 nkpesnqflv thggtwfeel qrkhrvehpi aaylikpvqr itkyqlllkd lqaccqegqg
     1441 eikdglevml nvpkkandal hlsmlegcdv ridtlgdvvl qdsftvwdpk qlirkgrdrh
     1501 iflfefhllf skevkdsagk vkyiyksrlm tselgvtehi egdeckfavw tgraptsdtr
     1561 vvlransmda kqlwvkrlre viqetyfsls mpkspakkss sqrssrdlee caslddsven
     1621 ldrnslasfg stnttdsdkt gvaevtwvia dhlaapgsre ltvtkgqqve vlengsnisg
     1681 vntaewthvr llvapgqvdp ppeglvptsa lkqpppvsnk tspsrkapaq qqqqqqqqqq
     1741 qqqqqqqqls snqiqstsps givtavvssa tigtggsssc sssssssvit gvpstgytvh
     1801 nvqnvvapll pdeaenvias dgstgtntss pgnkrrgfsg rkwlppplrk lsqgkvekss
     1861 gttitatpgs agisipatvt tattttiatp tppspppppp pltstatdrs slkknvsekr
     1921 fklpsgadqt rasrassvsi stltsaaasm rvsgsvtdvd ldttelvrtt ieaeteeeeg
     1981 eierseaele edttepddae tltyseqnga ddaedelelp ppmkpitepi lvgtsngssn
     2041 stistetggk srtserstki ldgattadla eieqivkerm eqhtenlerq slmrtpsgks
     2101 ssnvgsgged treeedslpt tmsiasitsp ssiattsspa htnpdehdpe stalakrqfv
     2161 irelvetekd yvndlkqive gymafmrdpe ceislpddlr ggkdkmvfgn ieaiyewhrd
     2221 fflkaldrcl erpeelgplf kryerklhmy vvycqnkpvs eyivseyidt yfeelrqklg
     2281 hrlqlcdlli kpvqritkyq lllrealrlt ertqrvaeie glraavhvmr vipkaandmm
     2341 dvgrlqgfdg kitaqgklll hgpllvtels nlpskgrewq vflfeqniif seavgkktqf
     2401 tnpayiykah iqvnkmslee cyedpekfvi rstdprkpsl gfsciaieen gprkqdwvdt
     2461 itailqtqrd flkaiqspia yqkeltkdpf rgvspespcr ggvlstippn lsntseeern
     2521 emtairaagk afaatttsit tisqqsrtga rsdqdslqtp spsksrlnfl egfksslrpr
     2581 spvrnnsipp ppslldtss
//
LOCUS       XP_034174674            2374 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 8 [Osmia lignaria lignaria].
ACCESSION   XP_034174674
VERSION     XP_034174674.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318783.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2374
                     /product="pre-mRNA processing factor 8"
                     /calculated_mol_wt=277031
     Region          53..2374
                     /region_name="PRP8"
                     /note="U5 snRNP spliceosome subunit [RNA processing and
                     modification]; COG5178"
                     /db_xref="CDD:227505"
     CDS             1..2374
                     /gene="Prp8"
                     /coded_by="XM_034318783.2:243..7367"
                     /db_xref="GeneID:117601673"
ORIGIN      
        1 msippyllgp npwatmmaqq qahaqlaaaq ahaqaavhaq aahhahmqai agpplpqmpk
       61 qpevlsedkl qekaqkwqql qskrfaekrk fgfvdaqked mppehirkii rdhgdmssrk
      121 yrhdkrvylg alkymphavm kllenmpmpw eqirdvkvly hitgaitfvn eipwviepvy
      181 iaqwgtmwim mrrekrdrrh fkrmrfppfd deeppldyad nvldveplea iqieldseed
      241 esvaswfyeh kplvgtkhvn gstyrrwnlt lpqmatlyrl anqlltdlvd qnffylfdpk
      301 sfftakalnm aipggpkfep lvkdsnaade dwnefndink iiirqpirte yriafpylyn
      361 nmphfvhlsw yhapnvvyik tedpdlpafy fdplinpish rnslktvepq ieddedfvls
      421 eevqpflqet plytdntang iallwaprpf ntrsgrtrra idiplvkswy rehcppgqpv
      481 kvrvsyqkll kyfvlnalkh rppkpqkkry lfrsfkstkf fqtttidwve aglqvcrqgy
      541 nmlnllihrk nlnylhldyn fnlkpvktlt tkerkksrfg nafhlcreil rltkliidsh
      601 vqyrlnnvda fqladglqyi fahvgqltgm yrykyklmrq irmckdlkhl iyyrfntgpv
      661 gkgpgcgfwa pgwrvwlffm rgitpllerw lgnllsrqfe grhskgvakt vtkqrveshf
      721 dlelrasvmh divdmmpegi kqnkartilq hlseawrcwk anipwkvpgl pipienmilr
      781 yvkmkadwwt ntahynreri rrgatvdktv ckknlgrltr lylkaeqerq hnylkdgpyi
      841 speeavaiyt ttvhwlesrr fapipfppls ykhdtkllil alerlkeays vksrlnqsqr
      901 eelglieqay dnphealsri krhlltqraf kevgiefmdl yshlipvydv eplekitday
      961 ldqylwyead krrlfppwvk psdtepppll vykwcqginn lqdvwdvseg ecnvlleskf
     1021 eklyekidlt llnrllrliv dhniadymta knnvvinykd mnhtnsygii rglqfasfia
     1081 qyyglvldll vlglqrasem agppqmpndf ltfqdvaset ahpirlycry vdrihlflrf
     1141 sadeardliq ryltehpdpn nenivgynnk kcwprdarmr lmkhdvnlgr avfwdiknrl
     1201 prstttiqwe nsfvsvyskd npnllfnmsg fecrilpkcr ttheefthrd gvwnlqneit
     1261 kertaqcflr vddeslqrfh nrvrqilmas gsttftkivn kwntaliglm tyfreavvnt
     1321 qelldllvkc enkiqtriki glnskmpsrf ppvvfytpke lgglgmlsmg hvlipqsdlr
     1381 wskqtdvgit hfrsgmshde dqlipnlyry iqpwesefid sqrvwaeyal krqeanaqnr
     1441 rltledleds wdrgiprint lfqkdrhtla ydkgwrirte fkqyqvlkqn pfwwthqrhd
     1501 gklwnlnnyr tdmiqalggv egilehtlfk gtyfptwegl fwekasgfee smkykkltna
     1561 qrsglnqipn rrftlwwspt inranvyvgf qvqldltgif mhgkiptlki sliqifrahl
     1621 wqkvhesivm dlcqvfdqel daleietvqk etihprksyk mnsscadill fsaykwnvsr
     1681 pslladskdl mdntttqkyw idvqlrwgdy dshdieryar akfldyttdn msiypsptgl
     1741 liaidlaynl hsaygnwfpg ckpliqqama kimkanpaly vlrerirkal qlysseptep
     1801 ylssqnygel fsnqiiwfvd dtnvyrvtih ktfegnlttk pingaififn prtgqlflki
     1861 ihtsvwagqk rlgqlakwkt aeevaalirs lpveeqpkqi ivtrkgmldp levhlldfpn
     1921 ivikgselql pfqaclkvek fgdlilkate pqmvlfnlyd dwlktissyt afsrlililr
     1981 alhvntertk vvlkpdktti tephhiwpsl tddewikvev qlkdlilady gkknnvnvas
     2041 ltqseirdii lgmeisapsa qrqqiaeiek qtkeqsqlta tttrtvnkhg deiitattsn
     2101 yetqtfsskt ewrvraisat nlhlrtnhiy vssddiketg ytyilpknvl kkfvtisdlr
     2161 aqiagylygi sppdnpqvke ircivmapqw gthqtvhlpn tlpnhqylke meplgwihtq
     2221 pnelpqlspq distharima enpiwdgekt ivitcsftpg scsltayklt psgfewgkqn
     2281 tdkgnnpkgy lpshyekvqm llsdrflgff mvpnqgswny nfmgvrhdpn mkyelqlanp
     2341 kefyheihrp ahflnfssle dgegvgadre dvfa
//
LOCUS       XP_034174684            2374 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 8 [Osmia lignaria lignaria].
ACCESSION   XP_034174684
VERSION     XP_034174684.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318793.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2374
                     /product="pre-mRNA processing factor 8"
                     /calculated_mol_wt=277031
     Region          53..2374
                     /region_name="PRP8"
                     /note="U5 snRNP spliceosome subunit [RNA processing and
                     modification]; COG5178"
                     /db_xref="CDD:227505"
     CDS             1..2374
                     /gene="Prp8"
                     /coded_by="XM_034318793.2:308..7432"
                     /db_xref="GeneID:117601673"
ORIGIN      
        1 msippyllgp npwatmmaqq qahaqlaaaq ahaqaavhaq aahhahmqai agpplpqmpk
       61 qpevlsedkl qekaqkwqql qskrfaekrk fgfvdaqked mppehirkii rdhgdmssrk
      121 yrhdkrvylg alkymphavm kllenmpmpw eqirdvkvly hitgaitfvn eipwviepvy
      181 iaqwgtmwim mrrekrdrrh fkrmrfppfd deeppldyad nvldveplea iqieldseed
      241 esvaswfyeh kplvgtkhvn gstyrrwnlt lpqmatlyrl anqlltdlvd qnffylfdpk
      301 sfftakalnm aipggpkfep lvkdsnaade dwnefndink iiirqpirte yriafpylyn
      361 nmphfvhlsw yhapnvvyik tedpdlpafy fdplinpish rnslktvepq ieddedfvls
      421 eevqpflqet plytdntang iallwaprpf ntrsgrtrra idiplvkswy rehcppgqpv
      481 kvrvsyqkll kyfvlnalkh rppkpqkkry lfrsfkstkf fqtttidwve aglqvcrqgy
      541 nmlnllihrk nlnylhldyn fnlkpvktlt tkerkksrfg nafhlcreil rltkliidsh
      601 vqyrlnnvda fqladglqyi fahvgqltgm yrykyklmrq irmckdlkhl iyyrfntgpv
      661 gkgpgcgfwa pgwrvwlffm rgitpllerw lgnllsrqfe grhskgvakt vtkqrveshf
      721 dlelrasvmh divdmmpegi kqnkartilq hlseawrcwk anipwkvpgl pipienmilr
      781 yvkmkadwwt ntahynreri rrgatvdktv ckknlgrltr lylkaeqerq hnylkdgpyi
      841 speeavaiyt ttvhwlesrr fapipfppls ykhdtkllil alerlkeays vksrlnqsqr
      901 eelglieqay dnphealsri krhlltqraf kevgiefmdl yshlipvydv eplekitday
      961 ldqylwyead krrlfppwvk psdtepppll vykwcqginn lqdvwdvseg ecnvlleskf
     1021 eklyekidlt llnrllrliv dhniadymta knnvvinykd mnhtnsygii rglqfasfia
     1081 qyyglvldll vlglqrasem agppqmpndf ltfqdvaset ahpirlycry vdrihlflrf
     1141 sadeardliq ryltehpdpn nenivgynnk kcwprdarmr lmkhdvnlgr avfwdiknrl
     1201 prstttiqwe nsfvsvyskd npnllfnmsg fecrilpkcr ttheefthrd gvwnlqneit
     1261 kertaqcflr vddeslqrfh nrvrqilmas gsttftkivn kwntaliglm tyfreavvnt
     1321 qelldllvkc enkiqtriki glnskmpsrf ppvvfytpke lgglgmlsmg hvlipqsdlr
     1381 wskqtdvgit hfrsgmshde dqlipnlyry iqpwesefid sqrvwaeyal krqeanaqnr
     1441 rltledleds wdrgiprint lfqkdrhtla ydkgwrirte fkqyqvlkqn pfwwthqrhd
     1501 gklwnlnnyr tdmiqalggv egilehtlfk gtyfptwegl fwekasgfee smkykkltna
     1561 qrsglnqipn rrftlwwspt inranvyvgf qvqldltgif mhgkiptlki sliqifrahl
     1621 wqkvhesivm dlcqvfdqel daleietvqk etihprksyk mnsscadill fsaykwnvsr
     1681 pslladskdl mdntttqkyw idvqlrwgdy dshdieryar akfldyttdn msiypsptgl
     1741 liaidlaynl hsaygnwfpg ckpliqqama kimkanpaly vlrerirkal qlysseptep
     1801 ylssqnygel fsnqiiwfvd dtnvyrvtih ktfegnlttk pingaififn prtgqlflki
     1861 ihtsvwagqk rlgqlakwkt aeevaalirs lpveeqpkqi ivtrkgmldp levhlldfpn
     1921 ivikgselql pfqaclkvek fgdlilkate pqmvlfnlyd dwlktissyt afsrlililr
     1981 alhvntertk vvlkpdktti tephhiwpsl tddewikvev qlkdlilady gkknnvnvas
     2041 ltqseirdii lgmeisapsa qrqqiaeiek qtkeqsqlta tttrtvnkhg deiitattsn
     2101 yetqtfsskt ewrvraisat nlhlrtnhiy vssddiketg ytyilpknvl kkfvtisdlr
     2161 aqiagylygi sppdnpqvke ircivmapqw gthqtvhlpn tlpnhqylke meplgwihtq
     2221 pnelpqlspq distharima enpiwdgekt ivitcsftpg scsltayklt psgfewgkqn
     2281 tdkgnnpkgy lpshyekvqm llsdrflgff mvpnqgswny nfmgvrhdpn mkyelqlanp
     2341 kefyheihrp ahflnfssle dgegvgadre dvfa
//
LOCUS       XP_034174729            1354 aa            linear   INV 03-SEP-2025
DEFINITION  inositol polyphosphate-4-phosphatase type I A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174729
VERSION     XP_034174729.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318838.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174729.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1354
                     /product="inositol polyphosphate-4-phosphatase type I A
                     isoform X1"
                     /calculated_mol_wt=153386
     Region          1..174
                     /region_name="PH_INPP4A_INPP4B"
                     /note="Type I inositol 3,4-bisphosphate 4-phosphatase and
                     Type II inositol 3,4-bisphosphate 4-phosphatase Pleckstrin
                     homology (PH) domain; cd13272"
                     /db_xref="CDD:270091"
     Region          197..305
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1354
                     /gene="LOC117601706"
                     /coded_by="XM_034318838.2:411..4475"
                     /db_xref="GeneID:117601706"
ORIGIN      
        1 mrfnkqellt latqpsqkfe kegilyvrer qegffrrses tgkksdnkcq kgkthktlrd
       61 lscvtastsk vslerwcrlr gnllfyfksr eqwseplgvi ileqcfvrve epsnqmpygf
      121 sivfdgglfq mlgansaeer dswlqalqla syecmrsqll alqqrieafs ghkhdtdiqm
      181 lrlqrgistd paeipmceis lacdnllcdg hgrppnpvle idvqvkgskt wikyartevv
      241 erssnpgflt tvsfrgndgl tsetkarita fdvrervsqt atpigsaivt lnaiqdtprl
      301 riplksaktt tvgfltinvw nleaedrgns testpskepa sgtnqqllsh rrsqslpprl
      361 gtkiklphqg qlkllfanpy iqtyrfhsgl ggdicvheim aesklsfqfp qhllaiwiqe
      421 ekellqevag mgelrepwhs kqvelldrhl hllhlysqak enlaafkgsy fkrssrkndr
      481 tlefaplnlh lqriwvhndt lnkcgfydfi tvgaftahsh kskngglirl lqalkesptr
      541 gnqfyqgtsk itmahdaiqa ikqlrrdvve amralmklak akqtsgmlpi cedmitktri
      601 llslwdpglv eealtfleey kvakvhdddg nedplnldfk tnhslspfkr itqqlnfdlk
      661 spdlddfvtp dtpecvqdlw vkrsenrngy atlpskssdg lnadrsnsnn ldlvekhskn
      721 tiheetnqsv vddaennyte nqkekekeek nrddgneeen sidndtavir ldndddnndd
      781 ddddddnndd nddnnddine dknenngere etkgdidpck rfldtqkmcn spsanyykpt
      841 depepwdltq lnieasvmcl vskvkflcgr csspavrlrn knsvgraqsm kksvatnrnk
      901 svkvvtiqpk neakneessk llenvdnrsn lrqypspvse mtmpafskak evcqavdsmt
      961 rviksnstgq rnkftegldf asivdwtsel rpsmkklrqa mdgllktarl thsvfrvqed
     1021 skmaqrtcnv ryrrdvcfsq altslvtgim aklwcqrpdp mfllilttlg plvsfeglls
     1081 yygdeidmwg dmtvaiedth tvtftlircg ieqrlegncs vpfqqpmprv vgsrvaltvl
     1141 lpvpdaiysl lplvpssrqt isfnvtpvff nvginemasl aeslgttkpq eksnmdnfer
     1201 lneyylrfkk lnlptetast rlgarsplgq tlpelltdlk asvqakvnkn vkilqlssqi
     1261 crrmrglrft scksakdrta msvtleqvni lsseyhlaeh eytraldcmr segcrrentw
     1321 knigirkyaf nslqiltfpk lyrpptgtyg saqt
//
LOCUS       XP_034174738            1323 aa            linear   INV 03-SEP-2025
DEFINITION  inositol polyphosphate-4-phosphatase type I A isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174738
VERSION     XP_034174738.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318847.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174738.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="inositol polyphosphate-4-phosphatase type I A
                     isoform X2"
                     /calculated_mol_wt=150080
     Region          1..143
                     /region_name="PH_INPP4A_INPP4B"
                     /note="Type I inositol 3,4-bisphosphate 4-phosphatase and
                     Type II inositol 3,4-bisphosphate 4-phosphatase Pleckstrin
                     homology (PH) domain; cd13272"
                     /db_xref="CDD:270091"
     Region          166..274
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1323
                     /gene="LOC117601706"
                     /coded_by="XM_034318847.2:411..4382"
                     /db_xref="GeneID:117601706"
ORIGIN      
        1 mrfnkqellt latqpsqkfe kegilyvrer qegffrrsei slerwcrlrg nllfyfksre
       61 qwseplgvii leqcfvrvee psnqmpygfs ivfdgglfqm lgansaeerd swlqalqlas
      121 yecmrsqlla lqqrieafsg hkhdtdiqml rlqrgistdp aeipmceisl acdnllcdgh
      181 grppnpvlei dvqvkgsktw ikyartevve rssnpgfltt vsfrgndglt setkaritaf
      241 dvrervsqta tpigsaivtl naiqdtprlr iplksakttt vgfltinvwn leaedrgnst
      301 estpskepas gtnqqllshr rsqslpprlg tkiklphqgq lkllfanpyi qtyrfhsglg
      361 gdicvheima esklsfqfpq hllaiwiqee kellqevagm gelrepwhsk qvelldrhlh
      421 llhlysqake nlaafkgsyf krssrkndrt lefaplnlhl qriwvhndtl nkcgfydfit
      481 vgaftahshk skngglirll qalkesptrg nqfyqgtski tmahdaiqai kqlrrdvvea
      541 mralmklaka kqtsgmlpic edmitktril lslwdpglve ealtfleeyk vakvhdddgn
      601 edplnldfkt nhslspfkri tqqlnfdlks pdlddfvtpd tpecvqdlwv krsenrngya
      661 tlpskssdgl nadrsnsnnl dlvekhsknt iheetnqsvv ddaennyten qkekekeekn
      721 rddgneeens idndtavirl dndddnnddd dddddnnddn ddnnddined knenngeree
      781 tkgdidpckr fldtqkmcns psanyykptd epepwdltql nieasvmclv skvkflcgrc
      841 sspavrlrnk nsvgraqsmk ksvatnrnks vkvvtiqpkn eakneesskl lenvdnrsnl
      901 rqypspvsem tmpafskake vcqavdsmtr viksnstgqr nkftegldfa sivdwtselr
      961 psmkklrqam dgllktarlt hsvfrvqeds kmaqrtcnvr yrrdvcfsqa ltslvtgima
     1021 klwcqrpdpm fllilttlgp lvsfegllsy ygdeidmwgd mtvaiedtht vtftlircgi
     1081 eqrlegncsv pfqqpmprvv gsrvaltvll pvpdaiysll plvpssrqti sfnvtpvffn
     1141 vginemasla eslgttkpqe ksnmdnferl neyylrfkkl nlptetastr lgarsplgqt
     1201 lpelltdlka svqakvnknv kilqlssqic rrmrglrfts cksakdrtam svtleqvnil
     1261 sseyhlaehe ytraldcmrs egcrrentwk nigirkyafn slqiltfpkl yrpptgtygs
     1321 aqt
//
LOCUS       XP_034174785            1044 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitination factor E4A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174785
VERSION     XP_034174785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="ubiquitination factor E4A isoform X1"
                     /calculated_mol_wt=119346
     Region          312..948
                     /region_name="Ufd2P_core"
                     /note="Ubiquitin elongating factor core; pfam10408"
                     /db_xref="CDD:463080"
     Region          967..1036
                     /region_name="RING-Ubox_UBE4A"
                     /note="U-box domain, a modified RING finger, found in
                     ubiquitin conjugation factor E4 A (UBE4A) and similar
                     proteins; cd16657"
                     /db_xref="CDD:438319"
     CDS             1..1044
                     /gene="Ube4A"
                     /coded_by="XM_034318894.2:1..3135"
                     /db_xref="GeneID:117601739"
ORIGIN      
        1 mcdnvnnnpf aglfstinda vsfssqnqti inesnrvnyi aqledknvdn tkeitnfqds
       61 kdfrndsqvn rllgdifgit lhiiksneeq krklvfvnvd sieqavferl mlsdlesklv
      121 pvgnsqdtvn dshtleklvm yylfesycrl qryqnkiecs diiqkirqvi lqnaavalqe
      181 pklfeeqeih nqfialcmde trpklelmsf tngivselqa eneknasdai vasftpildi
      241 ifkeaaqsnl vffrqywfti lnlfsaiepl aklviehstp ksnqgrayad tllgsllsls
      301 clpkaigepf yffdkplqqs sttvegniwt aldalneslq kvfhlllkcs ievrhltlqw
      361 igdclhsnan rgklwnaqnd vvfstmlcvs dgfmlnlgnv llrlcqpfci kqnefkvpki
      421 dptycaaevn nenecadqvi hlkgmssetc liptsesgak pvaktfgfit ecfflthral
      481 dlgyrvvldk flrtnqslvr iqrvyqdaqs ggrsevleti termeeemtk ylslrasllv
      541 pemlkllakf hattafwlvq vylddvrige tepsdyipke ckvvtfplpe tvpdtlrcip
      601 efvventirf lyilcrlnpn vfeeqgpsfl tpilteiivl mesqqrlynp hlrarlaegl
      661 eallpttdet mkpitpslgk fhreqlfith pyrqhivsnl lkvfvsiemt gqsvqfeqkf
      721 nyrrpmyvvm eylwklpehr nnfislaeea eanmeaaqpp lflrfinllm ndavflldea
      781 lssmaqlrql iqaresgewn klpqherdqq ahyllhlgmt arfnnilgrk tiytlkmltt
      841 eiksifchpt mvdriasmln ylllqlvgpn kknlkvngqk eyafnpanlv lniceiyinl
      901 sqnksftlav sqdgrsyspe lfkladkvlv higgvgmlgd ldqfannvea aandkkeede
      961 ilidapdefl dpimstlmid pvilpssgit idrqtiarhl lsdqtdpfnr spltmdmvks
     1021 nielqrrvqe wiqekkqeka rsrh
//
LOCUS       XP_034174794            1019 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitination factor E4A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034174794
VERSION     XP_034174794.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318903.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1019
                     /product="ubiquitination factor E4A isoform X2"
                     /calculated_mol_wt=116451
     Region          312..923
                     /region_name="Ufd2P_core"
                     /note="Ubiquitin elongating factor core; pfam10408"
                     /db_xref="CDD:463080"
     Region          942..1011
                     /region_name="RING-Ubox_UBE4A"
                     /note="U-box domain, a modified RING finger, found in
                     ubiquitin conjugation factor E4 A (UBE4A) and similar
                     proteins; cd16657"
                     /db_xref="CDD:438319"
     CDS             1..1019
                     /gene="Ube4A"
                     /coded_by="XM_034318903.2:1..3060"
                     /db_xref="GeneID:117601739"
ORIGIN      
        1 mcdnvnnnpf aglfstinda vsfssqnqti inesnrvnyi aqledknvdn tkeitnfqds
       61 kdfrndsqvn rllgdifgit lhiiksneeq krklvfvnvd sieqavferl mlsdlesklv
      121 pvgnsqdtvn dshtleklvm yylfesycrl qryqnkiecs diiqkirqvi lqnaavalqe
      181 pklfeeqeih nqfialcmde trpklelmsf tngivselqa eneknasdai vasftpildi
      241 ifkeaaqsnl vffrqywfti lnlfsaiepl aklviehstp ksnqgrayad tllgsllsls
      301 clpkaigepf yffdkplqqs sttvegniwt aldalneslq kvfhlllkcs ievrhltlqw
      361 igdclhsnan rgklwnaqnd vvfstmlcvs dgfmlnlgnv llrlcqpfci kqnefkvpki
      421 dptycaaevn nenecadqvi hlkgmssetc liptsesgak pvaktfgfit ecfflthral
      481 dlgyrvvldk flrtnqslvr iqrvyqdaqs ggrsevleti termeeemtk ylslrasllv
      541 pemlkllakf hattafwlvq vylddvrige tepsdyipke ckvvtfplpe tvpdtlrcip
      601 efvventirf lyilcrlnpn vfeeqgpsfl tpilteiivl mesqqrlynp hlrarlaegl
      661 eallpttdet mkpitpslgk fhreqlfith pyrqhivsnl lkvfvsiemt gqsvqfeqkf
      721 nyrrpmyvvm eylwklpehr nnfislaeea eanmeaaqpp lflrfinllm ndavflldea
      781 lssmaqlrql iqaresgewn klpqherdqq ahyllhlgmt arfnnildri asmlnylllq
      841 lvgpnkknlk vngqkeyafn panlvlnice iyinlsqnks ftlavsqdgr syspelfkla
      901 dkvlvhiggv gmlgdldqfa nnveaaandk keedeilida pdefldpims tlmidpvilp
      961 ssgitidrqt iarhllsdqt dpfnrspltm dmvksnielq rrvqewiqek kqekarsrh
//
LOCUS       XP_034181268             964 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181268
VERSION     XP_034181268.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325377.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181268.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..964
                     /product="discoidin domain-containing receptor 2 isoform
                     X1"
                     /calculated_mol_wt=108854
     Region          36..186
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(77,108,116)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          675..960
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(684..687,690,692,716,718,748,763..766,810,814..815,
                     817,827..828)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..964
                     /gene="LOC117604852"
                     /coded_by="XM_034325377.2:50..2944"
                     /db_xref="GeneID:117604852"
ORIGIN      
        1 mrrtlsvvsf gmilvaassp reqsteknif gqcilplgme egkipddait asssyetksv
       61 gpqnarirqe knggawcpka qissaireyl eidltrnhli awtetqgrfg ngqgqeyaea
      121 ffleywrdsk whqyknlkgd rvlrgnsnty lvekqkldlp fiasrvrfvp ysqhprtvcm
      181 rveiygcvwe qyviryqapk gsnigpggcn vqdssydgie tdsllidglg qltdgilgei
      241 leifscsttg tnwigwserd tvqlifhfqe prefnscsvh vahlpeleiq mfstlrvwfs
      301 vdgenyqsep ekleassnns nsstggtilt siplqsrigr svkieftltq rkwllvsein
      361 fhtvgdsskn rstdtyfsid rssfrtnenq selrstsmia sksietisgl neteiyeihq
      421 ddstpdafpv gtsqtyiglv sgsltiivlf ltcttflikq rgrnkvallq khtallcdss
      481 apgiaivpnv kdvkfsnslv nglslvrkpi tivasndsrd rldsqtnptr aitnfdhhpr
      541 sivyetscyk lfseenlvlp essssalate pcspdfkrks sltknkgpef ttsatttttt
      601 ttpttttttt aktnqkvheg yyaatdilti kkrelpsenl spspspftpl sireksvrlp
      661 htllvdpcdv qrvsrhrlri ldklgegnfg lvhlceakgi asvergnvfe nrqtvivrsl
      721 wrgvvdslrl dftkdmqila mlrdfnvakm valveeepfg avfeygqfgd lyhflqsyen
      781 dfsygdrlnf isqiasgmky feslniphcd laarncivsq nltikvsdha mfckkydhhy
      841 yidgcnakip lrwmaweavl lgkrncetdv wsfavtvwei cmdcketpya dltseqvlen
      901 yarwyedden gstkkeknsp rilsrpihcs dslylmmnnc wkkeaedrps feeiylyler
      961 lald
//
LOCUS       XP_076546194             753 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546194
VERSION     XP_076546194.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690079.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..753
                     /product="discoidin domain-containing receptor 2 isoform
                     X2"
                     /calculated_mol_wt=85761
     Region          36..186
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(77,108,116)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          464..749
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(473..476,479,481,505,507,537,552..555,599,603..604,
                     606,616..617)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..753
                     /gene="LOC117604852"
                     /coded_by="XM_076690079.1:50..2311"
                     /db_xref="GeneID:117604852"
ORIGIN      
        1 mrrtlsvvsf gmilvaassp reqsteknif gqcilplgme egkipddait asssyetksv
       61 gpqnarirqe knggawcpka qissaireyl eidltrnhli awtetqgrfg ngqgqeyaea
      121 ffleywrdsk whqyknlkgd rvlrgnsnty lvekqkldlp fiasrvrfvp ysqhprtvcm
      181 rveiygcvwe qyviryqapk gsnigpggcn vqdssydgie tdsllidglg qltdgilgei
      241 leifscsttg tnwigwserd tvqlifhfqe prefnscsvh vahlpeleiq mfstlrvwfs
      301 vdgenyqsep ekleassnns nsstggtilt siplqsrigr svkieftltq rkwllvsein
      361 fhtdfkrkss ltknkgpeft tsattttttt tpttttttta ktnqkvhegy yaatdiltik
      421 krelpsenls pspspftpls ireksvrlph tllvdpcdvq rvsrhrlril dklgegnfgl
      481 vhlceakgia svergnvfen rqtvivrslw rgvvdslrld ftkdmqilam lrdfnvakmv
      541 alveeepfga vfeygqfgdl yhflqsyend fsygdrlnfi sqiasgmkyf eslniphcdl
      601 aarncivsqn ltikvsdham fckkydhhyy idgcnakipl rwmaweavll gkrncetdvw
      661 sfavtvweic mdcketpyad ltseqvleny arwyeddeng stkkeknspr ilsrpihcsd
      721 slylmmnncw kkeaedrpsf eeiylylerl ald
//
LOCUS       XP_076546195             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546195
VERSION     XP_076546195.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690080.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_076690080.1:1395..3938"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_034174802             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174802
VERSION     XP_034174802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318911.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_034318911.2:1333..3876"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_076546196             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546196
VERSION     XP_076546196.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690081.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_076690081.1:1362..3905"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_034174810             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174810
VERSION     XP_034174810.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_034318919.2:1102..3645"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_034174819             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174819
VERSION     XP_034174819.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318928.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_034318928.2:1292..3835"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_034174823             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174823
VERSION     XP_034174823.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318932.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_034318932.2:522..3065"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_034174837             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174837
VERSION     XP_034174837.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318946.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_034318946.2:481..3024"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_034174842             847 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174842
VERSION     XP_034174842.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318951.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..847
                     /product="SCY1-like protein bma isoform X1"
                     /calculated_mol_wt=95377
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..847
                     /gene="bma"
                     /coded_by="XM_034318951.2:252..2795"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmvrqqpp iclnlngppp qpptlspllq
      841 lpgqpsh
//
LOCUS       XP_034174850             820 aa            linear   INV 03-SEP-2025
DEFINITION  SCY1-like protein bma isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034174850
VERSION     XP_034174850.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318959.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="SCY1-like protein bma isoform X2"
                     /calculated_mol_wt=92661
     Region          30..333
                     /region_name="PK_SCY1_like"
                     /note="Pseudokinase domain of Scy1-like proteins; cd14011"
                     /db_xref="CDD:270913"
     Region          453..483
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..517
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..820
                     /gene="bma"
                     /coded_by="XM_034318959.2:1395..3857"
                     /db_xref="GeneID:117601745"
ORIGIN      
        1 mfakskssgs apnvllsnpl qnfyeigkqs atagpenawr vydayakadr kevsifffdk
       61 rsveklykpk rketvteilr ngarqmerfs hpkilqaykv eecadslafv sepvlaslan
      121 vlayqeqran sigqstsnvt kqtssaashh rtiftkeyel ldieikygll qiteallflh
      181 vtrkvlhrnv cpasiiitkr gtwklsglef sekvnemsil lqpwtnrmpk maqpnldyia
      241 peiqqkkmgg fysdmysfgm ticaifnqgr pliqanhscs dylkqlenlk eqltvmlpmi
      301 plplqeavsr llhddpekrp taqvlsmiky fldpavhalq fldvskikdv lqkehfytnt
      361 lkgilpyvpk klwyqhiwty lqielesqev qsaalqpvly ivqnstqdey dqivfpslrs
      421 lfssrksvqg tvtllenlhl ilkktsreyv etevlpmlya sfensviqvq tasfiavsnv
      481 teyiddeair sivlpkllqa fekdsvdcri linvipciln rlekqkivdc ilpllfniel
      541 qepemivrvv kiyrlmlsdk kyglsvnwma tgampsllpq tinpslnleq fellfkvlqd
      601 mlnhieryqr nqltldnlsl ssperyrslr hqhstdnmhv ppfnipnlri eqrktssaed
      661 marknstnsi smtasaenma rknsfaavfg wfsssnndsn flkvantfts rrlsdntlma
      721 pkirvapsca sspgdtrggg lpirrhsstg pqerrgssin lspptgagmp itsssvpylv
      781 sssmnsirgs rrpsvsstss qqgagllqqv gssmyqffsk
//
LOCUS       XP_076546197             802 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601788 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546197
VERSION     XP_076546197.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690082.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..802
                     /product="uncharacterized protein LOC117601788 isoform X1"
                     /calculated_mol_wt=92569
     Region          6..76
                     /region_name="CaM_bind"
                     /note="Calcium-dependent calmodulin binding; pfam16025"
                     /db_xref="CDD:464981"
     CDS             1..802
                     /gene="LOC117601788"
                     /coded_by="XM_076690082.1:80..2488"
                     /db_xref="GeneID:117601788"
ORIGIN      
        1 meehsyvsci kingipilpp mitkdirqem snykesaini ekklavlraa kksnnydiyk
       61 envrnkskdv qlrkknyrfk edlvpvesrn gnfnetdvpv eisenlqysk rldtkyklen
      121 qtnltsntml rselvsvtns tdhylnetvs kkpevevvsn awkpqvpktl niipltlsds
      181 ncrrneeyqe sgsdntedtp klvrqgsyvl dapspillah mqmklasptc tpcseympts
      241 yantvcrkew kiaqgkiewe heaknkepfp aasltglstq trstscnrgr amrksvslqa
      301 kpkslsasty ssarsvdciq tmlaqehmnr snieinydkk ynvnssnekm pkfqrwkrcn
      361 nfstfnstyr fggslenldn ssgfskfteg qynnistknn ltidpafqlk sstasdkllt
      421 vykkvqvmhk kqmaelmsrq qkeqtllqqe fekqqflllt eirksfpels vsllsenils
      481 pafnqsennn ekffehidnn mkstkilesn lqqenrvehl qddstkmvqc pvdyiysein
      541 hctesctvkd srsteplitk psykidnfts ekkeseihth naksyenelt kemnsskrsn
      601 vsrelfplds ktihvpiier tmygtkhiea vniinayarg ylvrrmmrte rvialkntyk
      661 ealhcmlklh vdaplnrsef nflqrlqlqc daasmnivel faqspvkrmq iiaqdreikq
      721 frierptsar sysfatqrtl arkklkemee yqpasfvrtc lstrsrcqtw tsdvkerlis
      781 pnilyhsikr stsagtvrkp wr
//
LOCUS       XP_034174870             802 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601788 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174870
VERSION     XP_034174870.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174870.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..802
                     /product="uncharacterized protein LOC117601788 isoform X1"
                     /calculated_mol_wt=92569
     Region          6..76
                     /region_name="CaM_bind"
                     /note="Calcium-dependent calmodulin binding; pfam16025"
                     /db_xref="CDD:464981"
     CDS             1..802
                     /gene="LOC117601788"
                     /coded_by="XM_034318979.2:120..2528"
                     /db_xref="GeneID:117601788"
ORIGIN      
        1 meehsyvsci kingipilpp mitkdirqem snykesaini ekklavlraa kksnnydiyk
       61 envrnkskdv qlrkknyrfk edlvpvesrn gnfnetdvpv eisenlqysk rldtkyklen
      121 qtnltsntml rselvsvtns tdhylnetvs kkpevevvsn awkpqvpktl niipltlsds
      181 ncrrneeyqe sgsdntedtp klvrqgsyvl dapspillah mqmklasptc tpcseympts
      241 yantvcrkew kiaqgkiewe heaknkepfp aasltglstq trstscnrgr amrksvslqa
      301 kpkslsasty ssarsvdciq tmlaqehmnr snieinydkk ynvnssnekm pkfqrwkrcn
      361 nfstfnstyr fggslenldn ssgfskfteg qynnistknn ltidpafqlk sstasdkllt
      421 vykkvqvmhk kqmaelmsrq qkeqtllqqe fekqqflllt eirksfpels vsllsenils
      481 pafnqsennn ekffehidnn mkstkilesn lqqenrvehl qddstkmvqc pvdyiysein
      541 hctesctvkd srsteplitk psykidnfts ekkeseihth naksyenelt kemnsskrsn
      601 vsrelfplds ktihvpiier tmygtkhiea vniinayarg ylvrrmmrte rvialkntyk
      661 ealhcmlklh vdaplnrsef nflqrlqlqc daasmnivel faqspvkrmq iiaqdreikq
      721 frierptsar sysfatqrtl arkklkemee yqpasfvrtc lstrsrcqtw tsdvkerlis
      781 pnilyhsikr stsagtvrkp wr
//
LOCUS       XP_076546198             802 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601788 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546198
VERSION     XP_076546198.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690083.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..802
                     /product="uncharacterized protein LOC117601788 isoform X1"
                     /calculated_mol_wt=92569
     Region          6..76
                     /region_name="CaM_bind"
                     /note="Calcium-dependent calmodulin binding; pfam16025"
                     /db_xref="CDD:464981"
     CDS             1..802
                     /gene="LOC117601788"
                     /coded_by="XM_076690083.1:174..2582"
                     /db_xref="GeneID:117601788"
ORIGIN      
        1 meehsyvsci kingipilpp mitkdirqem snykesaini ekklavlraa kksnnydiyk
       61 envrnkskdv qlrkknyrfk edlvpvesrn gnfnetdvpv eisenlqysk rldtkyklen
      121 qtnltsntml rselvsvtns tdhylnetvs kkpevevvsn awkpqvpktl niipltlsds
      181 ncrrneeyqe sgsdntedtp klvrqgsyvl dapspillah mqmklasptc tpcseympts
      241 yantvcrkew kiaqgkiewe heaknkepfp aasltglstq trstscnrgr amrksvslqa
      301 kpkslsasty ssarsvdciq tmlaqehmnr snieinydkk ynvnssnekm pkfqrwkrcn
      361 nfstfnstyr fggslenldn ssgfskfteg qynnistknn ltidpafqlk sstasdkllt
      421 vykkvqvmhk kqmaelmsrq qkeqtllqqe fekqqflllt eirksfpels vsllsenils
      481 pafnqsennn ekffehidnn mkstkilesn lqqenrvehl qddstkmvqc pvdyiysein
      541 hctesctvkd srsteplitk psykidnfts ekkeseihth naksyenelt kemnsskrsn
      601 vsrelfplds ktihvpiier tmygtkhiea vniinayarg ylvrrmmrte rvialkntyk
      661 ealhcmlklh vdaplnrsef nflqrlqlqc daasmnivel faqspvkrmq iiaqdreikq
      721 frierptsar sysfatqrtl arkklkemee yqpasfvrtc lstrsrcqtw tsdvkerlis
      781 pnilyhsikr stsagtvrkp wr
//
LOCUS       XP_034174884             802 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601788 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174884
VERSION     XP_034174884.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174884.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..802
                     /product="uncharacterized protein LOC117601788 isoform X1"
                     /calculated_mol_wt=92569
     Region          6..76
                     /region_name="CaM_bind"
                     /note="Calcium-dependent calmodulin binding; pfam16025"
                     /db_xref="CDD:464981"
     CDS             1..802
                     /gene="LOC117601788"
                     /coded_by="XM_034318993.2:155..2563"
                     /db_xref="GeneID:117601788"
ORIGIN      
        1 meehsyvsci kingipilpp mitkdirqem snykesaini ekklavlraa kksnnydiyk
       61 envrnkskdv qlrkknyrfk edlvpvesrn gnfnetdvpv eisenlqysk rldtkyklen
      121 qtnltsntml rselvsvtns tdhylnetvs kkpevevvsn awkpqvpktl niipltlsds
      181 ncrrneeyqe sgsdntedtp klvrqgsyvl dapspillah mqmklasptc tpcseympts
      241 yantvcrkew kiaqgkiewe heaknkepfp aasltglstq trstscnrgr amrksvslqa
      301 kpkslsasty ssarsvdciq tmlaqehmnr snieinydkk ynvnssnekm pkfqrwkrcn
      361 nfstfnstyr fggslenldn ssgfskfteg qynnistknn ltidpafqlk sstasdkllt
      421 vykkvqvmhk kqmaelmsrq qkeqtllqqe fekqqflllt eirksfpels vsllsenils
      481 pafnqsennn ekffehidnn mkstkilesn lqqenrvehl qddstkmvqc pvdyiysein
      541 hctesctvkd srsteplitk psykidnfts ekkeseihth naksyenelt kemnsskrsn
      601 vsrelfplds ktihvpiier tmygtkhiea vniinayarg ylvrrmmrte rvialkntyk
      661 ealhcmlklh vdaplnrsef nflqrlqlqc daasmnivel faqspvkrmq iiaqdreikq
      721 frierptsar sysfatqrtl arkklkemee yqpasfvrtc lstrsrcqtw tsdvkerlis
      781 pnilyhsikr stsagtvrkp wr
//
LOCUS       XP_076546199             705 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601788 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546199
VERSION     XP_076546199.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690084.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..705
                     /product="uncharacterized protein LOC117601788 isoform X2"
                     /calculated_mol_wt=81275
     Region          6..76
                     /region_name="CaM_bind"
                     /note="Calcium-dependent calmodulin binding; pfam16025"
                     /db_xref="CDD:464981"
     CDS             1..705
                     /gene="LOC117601788"
                     /coded_by="XM_076690084.1:80..2197"
                     /db_xref="GeneID:117601788"
ORIGIN      
        1 meehsyvsci kingipilpp mitkdirqem snykesaini ekklavlraa kksnnydiyk
       61 envrnkskdv qlrkknyrfk edlvpvesrn gnfnetdvpv eisenlqysk rldtkyklen
      121 qtnltsntml rselvsvtns tdhylnetvs kkpevevvsn awkpqvpktl niipltlsds
      181 ncrrneeyqe sgsdntedtp klvrqgsyvl dapspillah mqmklasptc tpcseympts
      241 yantvcrkew kiaqgkiewe heaknkepfp aasltglstq trstscnrgr amrksvslqa
      301 kpkslsasty ssarsvdciq tmlaqehmnr snieinydkk ynvnssnekm pkfqrwkrcn
      361 nfstfnstyr fggslenldn ssgfskfteg qynnistknn ltidpafqlk sstasdkllt
      421 vykkvqvmhk kqmaelmsrq qkeqtllqqe fekqqflllt eirksfpels vsllsenils
      481 pafnqsennn ekffehidnn mkstkilesn lqqenrvehl qddstkmvqc pvdyiysein
      541 hctesctvkd srsteplitk psykidnfts ekkeseihth naksyenelt kemnsskrsn
      601 vsrelfplds ktihvpiier tmygtkhiea vniinayarg ylvrrmmrte rvialkntyk
      661 ealhcmlklh vdaplnrsef nflqrlqlqd grvstcfics ymfiy
//
LOCUS       XP_076546201             788 aa            linear   INV 03-SEP-2025
DEFINITION  ecto-NOX disulfide-thiol exchanger 2 [Osmia lignaria lignaria].
ACCESSION   XP_076546201
VERSION     XP_076546201.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690086.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..788
                     /product="ecto-NOX disulfide-thiol exchanger 2"
                     /calculated_mol_wt=89549
     Region          306..385
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          <477..>691
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     CDS             1..788
                     /gene="LOC117602028"
                     /coded_by="XM_076690086.1:90..2456"
                     /db_xref="GeneID:117602028"
ORIGIN      
        1 maynfpgvpa aamapmgmgp mgplasiagp qsfmelaggq gfpplpppip lpgmndsple
       61 fstnknqppv aretsedgsd krgekndvrr drenrdnrdn rdnrrsrser sdrrerdrer
      121 reernerdrr eerrpeerns mnstnnnnnn nnnnnnghnn cnsngneips sssssntsnt
      181 pnlgpqmeqa tgvwgmgvgy pvmgmgpmgp mgpmmgemtl gphmghthsy gpmsmgmmsh
      241 dgsmisaqil gpeqimndaa qimssalppp pttpqgtkei ihcksctlfp pnpnappptt
      301 rerppgcrti fvgglpenit etiiqeifer cgeittlrls kknfchirfv lensvdaaiy
      361 lsgyrvrigs sgdsantgrl hvdfaqardd qyewecrqrq lqreqrhrer vekerqraps
      421 spppvvhytd heasniceki kqddtfmkav qvvvtwlerg dctkrnantf ysmiqstnsh
      481 vrrlltekva yeeelqkake lmkgrmqgll iqfsqiervf taashkkvwd hftkaqrkni
      541 emwkkqssei kavqleetlm egegemevsd seeepqrkka rnqsdvhdds erlqalkeen
      601 dslrcqleay knevdllkse tkseidgkdk qmkmlqqtlk gmqeqlmqsr kqqaqddqki
      661 kdltvklnat kkeepresei itldkdddin eeprtpkqlv stsnfvqitq kdakliglia
      721 tflhihpfga svdylwsylq kmepgikpne vealmqrfpq vfkqdlfgig anmerrwqfs
      781 gfnvyrnh
//
LOCUS       XP_034174902             730 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601816 [Osmia lignaria lignaria].
ACCESSION   XP_034174902
VERSION     XP_034174902.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319011.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174902.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..730
                     /product="uncharacterized protein LOC117601816"
                     /calculated_mol_wt=83921
     Region          6..82
                     /region_name="THAP"
                     /note="The THAP domain is a putative DNA-binding domain
                     (DBD) and probably also binds a zinc ion; smart00980"
                     /db_xref="CDD:214951"
     Region          134..205
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); smart00868"
                     /db_xref="CDD:214871"
     Region          487..508
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(492,494,496,498..499,502..503,507,520,522,526..527,
                     530..531,534,543,546,548,550..551,554..555,558)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          515..534
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          538..559
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..730
                     /gene="LOC117601816"
                     /coded_by="XM_034319011.2:229..2421"
                     /db_xref="GeneID:117601816"
ORIGIN      
        1 meevtkcryi dcvdtrekeq ilhelpvcdt glcvrwlins ghvdligrdl dalrsmkffv
       61 cnnhftedcy lskgtlkena vpfphwsavs rtlkslktrr kvqvngqess meevsinqaa
      121 aeknapsefd vdqwcrtcat kkqnlvsmts kgkgtdmsll sklkllieid dedalptkmc
      181 decvdkleqs fkffqqiyva dntlrhvfpn srsnsmpkkp lyslsehmrr eqrekeekek
      241 ekeveqqdra pritrgifrr grgrprsrgl ggrgrgrqrs gqvamsiqsp isspmssrgn
      301 naivrtvqqe eeeeeeknrk neyeielhed erqgdtvfsl ltdtcrsdee ldwsdvlkvm
      361 nhqryrtseq dkskklekkt rldekmeekv etkietkves seaettlkvk tewseqvpie
      421 eqrkikvere evtevfvgdk ikpiiteeke vvvgrircef cekelkfrrq lqthlltdhs
      481 elsehmcvdc lacyesepll akhrslihve rryrcehcqe efpekrvlke hittrhscdl
      541 cglttftske dlsehvkshr evvstletit ehskgvdset isspksvgin egegegeeek
      601 ssaatiidtp snmivtfdsa sdeknvnpdp dpvpvpvpvp dlnpetassi drlkgnvsed
      661 lqgegesvac pdcneqmqnt lelsihrmrr hsvnrkdasc llcdnksfrs seeyeqhvld
      721 hckrlrispr
//
LOCUS       XP_034174924             677 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305215 [Osmia lignaria lignaria].
ACCESSION   XP_034174924
VERSION     XP_034174924.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319033.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174924.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..677
                     /product="uncharacterized protein LOC143305215"
                     /calculated_mol_wt=73939
     Region          127..268
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..677
                     /gene="LOC143305215"
                     /coded_by="XM_034319033.2:777..2810"
                     /db_xref="GeneID:143305215"
ORIGIN      
        1 mrrsgrtkgv lskwcssqna fvtperrkfr rvhglqlplh pqqvvgwivi lvivvntfai
       61 itpllgpslr sifsivisai flthffshlt vllldpadpr vrsqptnkii pefdrgkhsh
      121 viengrchlc nittdskrtk hcsicnkciv rfdhhckwln ncigarnyta flvclisail
      181 aslfiaglsa telllsfspd rfamlqvsna sghavptitp vsdavsvllv saigilsait
      241 aillihlclf hgyiaclglt tyeyvrkkrq rntvttaaav avaaaaaaaa aaaatatvta
      301 tatatatata tatatviata iatqtvdtlv aansttaepi dlsdsktirr dirrlvstdt
      361 dsclhcdrrn rridveidtr esssneiete ssfhrqredq rknfrlclpk rehqsesndk
      421 ppratippsh dsvgiratgs sspspvsccf aaansiavhc rstgqkrneq kkrsstddrr
      481 vdsldslrvs ttscetveri gkflrtylkr nrhrrrrrrs sssasrssvc askeniavaa
      541 aasstddsnq crrteikmnl estvslgell ettaisaprp ppsrlpplry slnelsvags
      601 sytrprrtfs frkraklkln shvtqsirls pilesefsks vtprsscssp lspssarstr
      661 sssspridrf vpsssgi
//
LOCUS       XP_034174980             649 aa            linear   INV 03-SEP-2025
DEFINITION  protein scabrous [Osmia lignaria lignaria].
ACCESSION   XP_034174980
VERSION     XP_034174980.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319089.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174980.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="protein scabrous"
                     /calculated_mol_wt=74159
     Region          49..>401
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          446..640
                     /region_name="FReD"
                     /note="Fibrinogen-related domains (FReDs); C terminal
                     globular domain of fibrinogen. Fibrinogen is involved in
                     blood clotting, being activated by thrombin to assemble
                     into fibrin clots. The N-termini of 2 times 3 chains come
                     together to form a globular...; cd00087"
                     /db_xref="CDD:238040"
     Site            557
                     /site_type="other"
                     /note="gamma-gamma dimer interface [polypeptide binding]"
                     /db_xref="CDD:238040"
     Site            order(585,587,589)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238040"
     Site            order(593,596..597,606..607)
                     /site_type="active"
                     /note="polymerization pocket [active]"
                     /db_xref="CDD:238040"
     CDS             1..649
                     /gene="LOC117601850"
                     /coded_by="XM_034319089.2:74..2023"
                     /db_xref="GeneID:117601850"
ORIGIN      
        1 mlvlprrvas rtrytdlgkm kletcsfwit livigavkke igaeenafda irslreqvna
       61 lldhrqqdyn alegslkrai ekntelfvlk devkelrkev ialrgggngn eakneklrvr
      121 wlgnavtelr gevaevlrtr naseefaers rmrgelalvk gdvaavgrgv rnlggriaki
      181 eavlgtirid iaavkerfsl lsrscadias qlstvqmevk slrcessila ngntvsgnsv
      241 tierdeklee dkresvrrgr nrieqelarr klakrqrtrv vdrlnslerk isstterrvr
      301 lekrvayene rwsklferve klekvqgels rqlsnvsena isrksigesl gtrliewlra
      361 leeavetnns stkqelvrlg vnaarkaael sltreelnnl rrtvqalsvt asrlqersds
      421 nlatsnnwtf eqqldeedry hlirqdlpen caeneledgp rvlrgrtsra mlvfcrkgwl
      481 visrridgsl dfdrtwneys igfgspvtey wigneilhrl trdnctslri dlldmngqrw
      541 raeyprfave skengyklrv ygyrgnatda lsyqngmpfs akdvdmdast shcaknyhgg
      601 wwfsrcqhan lngkyslglt wfrsdsnrwm siasaemsir qslddcrsr
//
LOCUS       XP_076546202             582 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601868 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546202
VERSION     XP_076546202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690087.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..582
                     /product="uncharacterized protein LOC117601868 isoform X1"
                     /calculated_mol_wt=68511
     CDS             1..582
                     /gene="LOC117601868"
                     /coded_by="XM_076690087.1:142..1890"
                     /db_xref="GeneID:117601868"
ORIGIN      
        1 mftvkqtqlf rgfkderwst kdkleqyrgi lklharerkq rlqdsvklkk riswqlgsyk
       61 edvnkyrqlt nrltngipeg yisnllqdrk dlrlkchgrk teqicvlvye gnhrkrcqld
      121 klryqkktkl kqcfqlqleh aqlcaeyaqe qetpwaenrl qqrlvtsyqr svakqnaaka
      181 invtyvamld vlkqdainhe rllnalkedr enqckailka timgqlatee tddiatkckr
      241 iarivwnnmk erermlaivr drvedlwsfa rslirteset iftmeitrgq tasnvtlekq
      301 ittleeifdk vkesllvrsy rellsrleeq mkqrtqlleq fnrnvkerdl llrkkneals
      361 alsnfehslv atteeyntdk nvlleqitiq kkrreehenl rkehgqllmd iraalqnmla
      421 mlfcvrrgsk vpkkqtdeld eqmvviekme tegltllstv srkvgalfgm snfefnkdre
      481 qrakdlyqty vsdyhsklkf edagfeptgk lflieriing iqteqadrar ffdssilhli
      541 svirilllgl fvehetidss vptraeiklr srqivetylr le
//
LOCUS       XP_034175054             542 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601868 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175054
VERSION     XP_034175054.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319163.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175054.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="uncharacterized protein LOC117601868 isoform X2"
                     /calculated_mol_wt=63906
     CDS             1..542
                     /gene="LOC117601868"
                     /coded_by="XM_034319163.2:142..1770"
                     /db_xref="GeneID:117601868"
ORIGIN      
        1 mftvkqtqlf rgfkderwst kdkleqyrgi lklharerkq rlqdsvklkk riswqlgsyk
       61 edvnkyrqlt nrltngipeg yisnllqdrk dlrlkchgrk teqicvlvye gnhrkrcqld
      121 klryqkktkl kqcfqlqleh aqlcaeyaqe qetpwaenrl qqrlvtsyqr svakqnaaka
      181 invtyvamld vlkqdainhe rllnalkedr enqckailka timgqlatee tddiatkckr
      241 iarivwnnmk erermlaivr drvedlwsfa rslirteset iftmeitrgq tasnvtlekq
      301 ittleeifdk vkesllvrsy rellsrleeq mkqrtqlleq fnrnvkerdl llrkkneals
      361 alsnfehslv atteeyntdk nvlleqitiq kkrreehenl rkehgqllmd iraalqnmla
      421 mlfcvrrgsk vpkkqtdeld eqmvviekme tegltllstv srkvgalfgm snfefnkdre
      481 qrakdlyqty vsdyhsklkf edagfeptgl fvehetidss vptraeiklr srqivetylr
      541 le
//
LOCUS       XP_034174993             568 aa            linear   INV 03-SEP-2025
DEFINITION  DExD-box helicase 52 [Osmia lignaria lignaria].
ACCESSION   XP_034174993
VERSION     XP_034174993.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319102.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..568
                     /product="DExD-box helicase 52"
                     /calculated_mol_wt=64848
     Region          143..506
                     /region_name="SrmB"
                     /note="Superfamily II DNA and RNA helicase [Replication,
                     recombination and repair]; COG0513"
                     /db_xref="CDD:440279"
     CDS             1..568
                     /gene="ais"
                     /coded_by="XM_034319102.2:145..1851"
                     /db_xref="GeneID:117601857"
ORIGIN      
        1 mdayelfrkl stgvkfdkkr fqtdaerfql lkkqlesipk nerksittfn dincsmmsmk
       61 rkhdeieedr enndelilln gisvpkngnk ikskklkisi teekglklkq ekinqfrnqh
      121 risitgsrip episefikls tcygvseqll nnvincgyvh ptpiqmqaip imlqnrqvla
      181 captgsgkta afllpiihcl ggpqkkgfra vilcptrela kqtyreclrl segynlrihi
      241 iskvnqalhk ygpkssqkfd ilittprrii fllnqdppai sfknvewliv deadklfedg
      301 trcfrdqlei iskactnekl cramfsatnt pivtkwcrhn lkglatvtvg hrnaatdlve
      361 qellfvgaer gklvalrnii qkgvlppvlv fvqskeraqe lfneliydgi nvdvihadrt
      421 qsqrdnvvrc fregkiwvli ctelmargid fkgvnlviny dfppsvisyv hrigrtgrag
      481 hrgkaitfft kqdttnlrsi aavmrqsgcn vpdymlamkk hskkekrkle hkalvrekis
      541 tipkfkrirn kermrelggn asnqkqhe
//
LOCUS       XP_034175013             507 aa            linear   INV 03-SEP-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175013
VERSION     XP_034175013.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="DDB1- and CUL4-associated factor 11 isoform X1"
                     /calculated_mol_wt=57004
     Region          130..166
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <132..465
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          174..216
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          222..258
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          263..305
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          394..434
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          440..464
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..507
                     /gene="LOC117601862"
                     /coded_by="XM_034319122.2:150..1673"
                     /db_xref="GeneID:117601862"
ORIGIN      
        1 mggvnsrdmd iahdpglaav lqylirsgql hiitsdqdds eeeyaansqp prvtsspnts
       61 rldkseisla tkqacgfvdn vdkhnlsvts miqrrslgps fsrgercris ssflpnkmhq
      121 vakyntkafc gsysedgrff ltasqdkflr lyrthdgefv efktipardv gwsildtafs
      181 pdgnyivyss wseclylwpv ygdsstqesl slcpeerrfg vfsvvfssdg reilsgandg
      241 yfyiydrech qrafrieahd ndvntvvfad dtsqilysag ddglckvwdr rtlsetdphp
      301 vgvlaghmdg ityidprgdg rylitnskdq tiklwdvraf sdhngeqntr kavanqnwdy
      361 rwqkvpkrlh karnmlegdt simtyrghsv lqtlirchfs pssitgqryi ytgcaagrvi
      421 iydvltgriv sslvghkgcv rdvswhpfhq eivstswdga ivswryagkt aldnsdseee
      481 tdaespprtl rrsqriaaqr akhaatc
//
LOCUS       XP_076546203             487 aa            linear   INV 03-SEP-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546203
VERSION     XP_076546203.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690088.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..487
                     /product="DDB1- and CUL4-associated factor 11 isoform X2"
                     /calculated_mol_wt=55520
     Region          110..146
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <112..445
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          154..196
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          202..238
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          243..285
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          374..414
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          420..444
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..487
                     /gene="LOC117601862"
                     /coded_by="XM_076690088.1:76..1539"
                     /db_xref="GeneID:117601862"
ORIGIN      
        1 mfviykyair llplsftffh frsifpllcm gfllfsffff lfqkseisla tkqacgfvdn
       61 vdkhnlsvts miqrrslgps fsrgercris ssflpnkmhq vakyntkafc gsysedgrff
      121 ltasqdkflr lyrthdgefv efktipardv gwsildtafs pdgnyivyss wseclylwpv
      181 ygdsstqesl slcpeerrfg vfsvvfssdg reilsgandg yfyiydrech qrafrieahd
      241 ndvntvvfad dtsqilysag ddglckvwdr rtlsetdphp vgvlaghmdg ityidprgdg
      301 rylitnskdq tiklwdvraf sdhngeqntr kavanqnwdy rwqkvpkrlh karnmlegdt
      361 simtyrghsv lqtlirchfs pssitgqryi ytgcaagrvi iydvltgriv sslvghkgcv
      421 rdvswhpfhq eivstswdga ivswryagkt aldnsdseee tdaespprtl rrsqriaaqr
      481 akhaatc
//
LOCUS       XP_076546204             458 aa            linear   INV 03-SEP-2025
DEFINITION  DDB1- and CUL4-associated factor 11 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546204
VERSION     XP_076546204.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690089.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..458
                     /product="DDB1- and CUL4-associated factor 11 isoform X3"
                     /calculated_mol_wt=51953
     Region          81..117
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <83..416
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          125..167
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          173..209
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          214..256
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          345..385
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          391..415
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..458
                     /gene="LOC117601862"
                     /coded_by="XM_076690089.1:162..1538"
                     /db_xref="GeneID:117601862"
ORIGIN      
        1 mgfllfsfff flfqkseisl atkqacgfvd nvdkhnlsvt smiqrrslgp sfsrgercri
       61 sssflpnkmh qvakyntkaf cgsysedgrf fltasqdkfl rlyrthdgef vefktipard
      121 vgwsildtaf spdgnyivys swseclylwp vygdsstqes lslcpeerrf gvfsvvfssd
      181 greilsgand gyfyiydrec hqrafrieah dndvntvvfa ddtsqilysa gddglckvwd
      241 rrtlsetdph pvgvlaghmd gityidprgd grylitnskd qtiklwdvra fsdhngeqnt
      301 rkavanqnwd yrwqkvpkrl hkarnmlegd tsimtyrghs vlqtlirchf spssitgqry
      361 iytgcaagrv iiydvltgri vsslvghkgc vrdvswhpfh qeivstswdg aivswryagk
      421 taldnsdsee etdaespprt lrrsqriaaq rakhaatc
//
LOCUS       XP_034175028             546 aa            linear   INV 03-SEP-2025
DEFINITION  G-protein coupled receptor Mth2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175028
VERSION     XP_034175028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="G-protein coupled receptor Mth2 isoform X1"
                     /calculated_mol_wt=62839
     Region          49..169
                     /region_name="Mth_Ecto"
                     /note="The ectodomain of Methuselah (Mth); Mth mutants
                     have a 35% increase in average lifespan and increased
                     resistance to several forms of stress, including heat,
                     starvation, and oxidative damage; The protein affected by
                     this mutation is related to G...; cl11637"
                     /db_xref="CDD:448329"
     Site            order(52,119,166)
                     /site_type="other"
                     /note="N-linked glycosylation sites [posttranslational
                     modification]"
                     /db_xref="CDD:119403"
     Site            140
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:119403"
     Region          216..488
                     /region_name="7tmB3_Methuselah-like"
                     /note="Methuselah-like subfamily B3, member of the class B
                     family of seven-transmembrane G protein-coupled receptors;
                     cd15039"
                     /db_xref="CDD:410632"
     Site            order(216,220,262,265..266,269,277,284,372..373,375,377,
                     438,441,455,459)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:410632"
     Region          216..236
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410632"
     Region          245..267
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410632"
     Region          277..304
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410632"
     Region          323..343
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410632"
     Region          369..398
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410632"
     Region          419..446
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410632"
     Region          451..477
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410632"
     CDS             1..546
                     /gene="LOC117601865"
                     /coded_by="XM_034319137.2:601..2241"
                     /db_xref="GeneID:117601865"
ORIGIN      
        1 mrhyivarvi sfvlidwlrg segkrietke dsfcrpfpsi vlegnkigrl engslvhnkl
       61 vypvnsyrvv gnetygcvcn vraclrkccr rdqilggndr pnctqlengq lardlaleer
      121 qlaneiqgis glsdffvlie dmlcpenkyr leperyeeda fvllengtlq tstskfaawt
      181 ycldwkdsfe kivvlvclst ddasssyeee kqqesykvgi ivsipfffvt flvyaiipel
      241 rnlygktlmc yvaclvvayt flvlvkffyy ftialcsaia fvihfsflas ffwlnvmcfd
      301 iwwtfggfrs lqgsvkqrer kkfliysvya wgcaflltgv civmdflpni pdhfvkpefg
      361 tqscwfntdk akaiyfygpm gvtvfcnicl fvstalkivq hkkdtahhlk gidsrrhddn
      421 kqwfnlylkl fivmginwsm eivswlcnns payiwyltdl tntlqgviif lifvwkdkir
      481 rlllkrfgch gnnvlsrnst rsayhssasr ttcttsavpl hekiityssd pasrnrttfi
      541 dnndcv
//
LOCUS       XP_034175038             496 aa            linear   INV 03-SEP-2025
DEFINITION  G-protein coupled receptor Mth2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175038
VERSION     XP_034175038.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319147.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175038.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="G-protein coupled receptor Mth2 isoform X2"
                     /calculated_mol_wt=56714
     Region          <28..131
                     /region_name="Mth_Ecto"
                     /note="The ectodomain of Methuselah (Mth); Mth mutants
                     have a 35% increase in average lifespan and increased
                     resistance to several forms of stress, including heat,
                     starvation, and oxidative damage; The protein affected by
                     this mutation is related to G...; cl11637"
                     /db_xref="CDD:448329"
     Region          157..438
                     /region_name="7tmB3_Methuselah-like"
                     /note="Methuselah-like subfamily B3, member of the class B
                     family of seven-transmembrane G protein-coupled receptors;
                     cd15039"
                     /db_xref="CDD:410632"
     Region          159..184
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410632"
     Site            order(164,168,210,213..214,217,227,234,322..323,325,327,
                     388,391,405,409)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:410632"
     Region          193..215
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410632"
     Region          227..254
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410632"
     Region          273..293
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410632"
     Region          319..348
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410632"
     Region          369..396
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410632"
     Region          401..427
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410632"
     CDS             1..496
                     /gene="LOC117601865"
                     /coded_by="XM_034319147.2:179..1669"
                     /db_xref="GeneID:117601865"
ORIGIN      
        1 mfsstvfvti ailaidaies lsrtnvivgk tlprvalccp yasrlsagrc fetnitifrf
       61 pslyrsndls llletpdrdc fdffirypcn asqsynldpe kypedafmll dngsiykqpe
      121 nvileeteyc fgitdtdayy vvlcfdddts qvkddtstei rivfpvgliv svpflfatfl
      181 vytllpelkn mhgrtlrgyi gsllvayvil fvvqissqdq isdplciafa fvihfsflas
      241 ffwlnvmcfd iwwtfggfrs lqgsvkqrer kkfliysvya wgcaflltgv civmdflpni
      301 pdhfvkpefg tqscwfntdk akaiyfygpm gvtvfcnicl fvstalkivq hkkdtahhlk
      361 gidsrrhddn kqwfnlylkl fivmginwsm eivswlcnns payiwyltdl tntlqgviif
      421 lifvwkdkir rlllkrfgch gnnvlsrnst rsayhssasr ttcttsavpl hekiityssd
      481 pasrnrttfi dnndcv
//
LOCUS       XP_034175069             522 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat domain-containing protein hfw isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034175069
VERSION     XP_034175069.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319178.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="leucine-rich repeat domain-containing protein
                     hfw isoform X1"
                     /calculated_mol_wt=59531
     Region          144..203
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          146..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <172..>394
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          172..192
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          193..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          313..330
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          331..354
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          356..382
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..522
                     /gene="hfw"
                     /coded_by="XM_034319178.2:391..1959"
                     /db_xref="GeneID:117601869"
ORIGIN      
        1 mkiflkcvli dgvftdsiar lykmkmqwfa immclyagva sarteedqqt sssaaitgis
       61 sslleqcfyq qfsecpnaki tgcsckkivl vhnnpegnav lccnldvqnf eselscagfp
      121 snisyihirn atldifnvse irwrrlksla itdgrinrvk gqfrmmtptv clnlsnnali
      181 evennsltrl aqlttldlsy nnlthlpaln tmngrefwld isgtntlwch diyqyinktg
      241 ekqinfnren etvcsasktw hwfntteqvp lkqvrylsll qtecpkgdsw qcqcnfrrld
      301 ivegkpptla vnvdcsgiqi telpeklprn tialnvsynn itvlddlstn pcyedirefy
      361 adynnissin klegskfldn yallslrynk ikslptyils pnthdksfis srlvklggne
      421 lhcdcntaky lkvwlqtril dsdevlcenv kekvidleps kmcvypgdwt dyiyyiiate
      481 vvllisliak vsydywifkt agylpwpank mpklpcdwlc et
//
LOCUS       XP_034175079             516 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat domain-containing protein hfw isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034175079
VERSION     XP_034175079.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319188.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="leucine-rich repeat domain-containing protein
                     hfw isoform X2"
                     /calculated_mol_wt=58956
     Region          138..197
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          140..162
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <166..>388
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          166..186
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          187..201
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          307..324
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          325..348
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          350..376
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..516
                     /gene="hfw"
                     /coded_by="XM_034319188.2:16..1566"
                     /db_xref="GeneID:117601869"
ORIGIN      
        1 mykvyssikk feinfifsnm qwfaimmcly agvasartee dqqtsssaai tgissslleq
       61 cfyqqfsecp nakitgcsck kivlvhnnpe gnavlccnld vqnfeselsc agfpsnisyi
      121 hirnatldif nvseirwrrl kslaitdgri nrvkgqfrmm tptvclnlsn nalievenns
      181 ltrlaqlttl dlsynnlthl palntmngre fwldisgtnt lwchdiyqyi nktgekqinf
      241 nrenetvcsa sktwhwfntt eqvplkqvry lsllqtecpk gdswqcqcnf rrldivegkp
      301 ptlavnvdcs giqitelpek lprntialnv synnitvldd lstnpcyedi refyadynni
      361 ssinklegsk fldnyallsl rynkikslpt yilspnthdk sfissrlvkl ggnelhcdcn
      421 takylkvwlq trildsdevl cenvkekvid lepskmcvyp gdwtdyiyyi iatevvllis
      481 liakvsydyw ifktagylpw pankmpklpc dwlcet
//
LOCUS       XP_034175089             497 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat domain-containing protein hfw isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034175089
VERSION     XP_034175089.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="leucine-rich repeat domain-containing protein
                     hfw isoform X3"
                     /calculated_mol_wt=56615
     Region          119..178
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          121..143
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <147..>369
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          147..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          168..182
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          288..305
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          306..329
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          331..357
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..497
                     /gene="hfw"
                     /coded_by="XM_034319198.2:129..1622"
                     /db_xref="GeneID:117601869"
ORIGIN      
        1 mqwfaimmcl yagvasarte edqqtsssaa itgissslle qcfyqqfsec pnakitgcsc
       61 kkivlvhnnp egnavlccnl dvqnfesels cagfpsnisy ihirnatldi fnvseirwrr
      121 lkslaitdgr inrvkgqfrm mtptvclnls nnalievenn sltrlaqltt ldlsynnlth
      181 lpalntmngr efwldisgtn tlwchdiyqy inktgekqin fnrenetvcs asktwhwfnt
      241 teqvplkqvr ylsllqtecp kgdswqcqcn frrldivegk pptlavnvdc sgiqitelpe
      301 klprntialn vsynnitvld dlstnpcyed irefyadynn issinklegs kfldnyalls
      361 lrynkikslp tyilspnthd ksfissrlvk lggnelhcdc ntakylkvwl qtrildsdev
      421 lcenvkekvi dlepskmcvy pgdwtdyiyy iiatevvlli sliakvsydy wifktagylp
      481 wpankmpklp cdwlcet
//
LOCUS       XP_034175096             497 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat domain-containing protein hfw isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034175096
VERSION     XP_034175096.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319205.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="leucine-rich repeat domain-containing protein
                     hfw isoform X3"
                     /calculated_mol_wt=56615
     Region          119..178
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          121..143
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <147..>369
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          147..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          168..182
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          288..305
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          306..329
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          331..357
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..497
                     /gene="hfw"
                     /coded_by="XM_034319205.2:25..1518"
                     /db_xref="GeneID:117601869"
ORIGIN      
        1 mqwfaimmcl yagvasarte edqqtsssaa itgissslle qcfyqqfsec pnakitgcsc
       61 kkivlvhnnp egnavlccnl dvqnfesels cagfpsnisy ihirnatldi fnvseirwrr
      121 lkslaitdgr inrvkgqfrm mtptvclnls nnalievenn sltrlaqltt ldlsynnlth
      181 lpalntmngr efwldisgtn tlwchdiyqy inktgekqin fnrenetvcs asktwhwfnt
      241 teqvplkqvr ylsllqtecp kgdswqcqcn frrldivegk pptlavnvdc sgiqitelpe
      301 klprntialn vsynnitvld dlstnpcyed irefyadynn issinklegs kfldnyalls
      361 lrynkikslp tyilspnthd ksfissrlvk lggnelhcdc ntakylkvwl qtrildsdev
      421 lcenvkekvi dlepskmcvy pgdwtdyiyy iiatevvlli sliakvsydy wifktagylp
      481 wpankmpklp cdwlcet
//
LOCUS       XP_034175111             490 aa            linear   INV 03-SEP-2025
DEFINITION  locomotion-related protein hikaru genki isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175111
VERSION     XP_034175111.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319220.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="locomotion-related protein hikaru genki isoform
                     X1"
                     /calculated_mol_wt=54261
     Region          31..>116
                     /region_name="PHA02831"
                     /note="EEV host range protein; Provisional"
                     /db_xref="CDD:165176"
     Region          <260..484
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..490
                     /gene="hig"
                     /coded_by="XM_034319220.2:4..1476"
                     /db_xref="GeneID:117601884"
ORIGIN      
        1 mfidkrekrk siferayrsi lairylllfv gfasainicn akqidtdgfq rgckleglhp
       61 llivtyknit vsvdkitvph gervtircre lgkykligds llhcrntswt gklpycsptt
      121 aisnytedvp ptivfglptg saavepsgal avfpgsilhl eclfarklgn pewtwtstfr
      181 qyltgwaisa rerdwkyrls iyyaktqdsg vytcstprgl snsirvrivd vqcpvlnlpe
      241 ppligkiega hmghgavfec pvgfrlegaa gitcqyngkw sadvprceti ecpamdivtd
      301 awlqlieynn sfggkavfac mwghkllgsq svecqgdgsw sgampicaei tcpppsipks
      361 griveqtnrh hsgrkqhhqq qriraykvga lvrfaclpgh qllgeasiic tengtwshps
      421 pvckvrcpyp gdpphgriap lkfwykpgdn iqvtcspgyv tplepvrkpt crengiwsap
      481 pppcrsykdv
//
LOCUS       XP_034175119             439 aa            linear   INV 03-SEP-2025
DEFINITION  locomotion-related protein hikaru genki isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175119
VERSION     XP_034175119.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319228.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..439
                     /product="locomotion-related protein hikaru genki isoform
                     X2"
                     /calculated_mol_wt=48388
     Region          <209..433
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..439
                     /gene="hig"
                     /coded_by="XM_034319228.2:189..1508"
                     /db_xref="GeneID:117601884"
ORIGIN      
        1 mpstfsqcss ahgycrefik rvdkitvphg ervtircrel gkykligdsl lhcrntswtg
       61 klpycsptta isnytedvpp tivfglptgs aavepsgala vfpgsilhle clfarklgnp
      121 ewtwtstfrq yltgwaisar erdwkyrlsi yyaktqdsgv ytcstprgls nsirvrivdv
      181 qcpvlnlpep pligkiegah mghgavfecp vgfrlegaag itcqyngkws advprcetie
      241 cpamdivtda wlqlieynns fggkavfacm wghkllgsqs vecqgdgsws gampicaeit
      301 cpppsipksg riveqtnrhh sgrkqhhqqq riraykvgal vrfaclpghq llgeasiict
      361 engtwshpsp vckvrcpypg dpphgriapl kfwykpgdni qvtcspgyvt plepvrkptc
      421 rengiwsapp ppcrsykdv
//
LOCUS       XP_076546206             423 aa            linear   INV 03-SEP-2025
DEFINITION  locomotion-related protein hikaru genki isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546206
VERSION     XP_076546206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690091.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..423
                     /product="locomotion-related protein hikaru genki isoform
                     X3"
                     /calculated_mol_wt=46769
     Region          31..>116
                     /region_name="PHA02831"
                     /note="EEV host range protein; Provisional"
                     /db_xref="CDD:165176"
     Region          130..214
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          <232..421
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..423
                     /gene="hig"
                     /coded_by="XM_076690091.1:4..1275"
                     /db_xref="GeneID:117601884"
ORIGIN      
        1 mfidkrekrk siferayrsi lairylllfv gfasainicn akqidtdgfq rgckleglhp
       61 llivtyknit vsvdkitvph gervtircre lgkykligds llhcrntswt gklpycsptt
      121 aisnytedvp ptivfglptg saavepsgal avfpgsilhl eclfarklgn pewtwtstfr
      181 qyltgwaisa rerdwkyrls iyyaktqdsg vytcstprgl snsirvrivd vqcpvlnlpe
      241 ppligkiega hmghgavfec pvgfrlegaa gitcqyngkw sadvprceti ecpamdivtd
      301 awlqlieynn sfggkavfac mwghkllgsq svecqgdgsw sgampicaei tcpppsipks
      361 griveqtnrh hsgrkqhhqq qriraykvga lvrfaclpgh qllgeasiic tengtwshps
      421 pvk
//
LOCUS       XP_034174960             471 aa            linear   INV 03-SEP-2025
DEFINITION  dihydrouridine synthase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174960
VERSION     XP_034174960.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319069.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174960.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="dihydrouridine synthase 1 isoform X1"
                     /calculated_mol_wt=53988
     Region          23..246
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(27..29,53,82,108,150,178,211,213,235..236)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(82,111..112,150,152,177..178,180..181,212..213,236)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(111,152,178,180)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(112,150,177,181,212..213)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     CDS             1..471
                     /gene="Dus1"
                     /coded_by="XM_034319069.2:219..1634"
                     /db_xref="GeneID:117601822"
ORIGIN      
        1 mssvsaalae paniwetvlg spryivapmv daselawrll srrhgahlcy tpmlhssvfc
       61 rdpkyrreal astaedrpli vqfcgndpnt lleaallaep ycdavdinig cpqaiakrgr
      121 ygaflqddwd llrrivstls kglrvpvtck lrvfaeiekt veyarmlena garlltvhgr
      181 treqkgpltg laswdhikav rqavripvfa ngniqslqdv ekcmeetgvh gvmsaegnly
      241 npfifearyp psweaaleyl dlverypapa syirghlfkl fqhtlclaen keerenlarn
      301 ssmesfksvv ytlrdrylpy hegrlrwqqe tsdynlelpp wlcqpyarhl peecvrklet
      361 gkletqreet ikrkfkdddg neisrkrlkk lrriarrpnr ptvvikrgse lchdcpnpvg
      421 lkcvhklcrq ccrnkcften ldcighrnlt ktrrqmaief aakrktvhdq i
//
LOCUS       XP_076546208             471 aa            linear   INV 03-SEP-2025
DEFINITION  dihydrouridine synthase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546208
VERSION     XP_076546208.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690093.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="dihydrouridine synthase 1 isoform X1"
                     /calculated_mol_wt=53988
     Region          23..246
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(27..29,53,82,108,150,178,211,213,235..236)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(82,111..112,150,152,177..178,180..181,212..213,236)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(111,152,178,180)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(112,150,177,181,212..213)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     CDS             1..471
                     /gene="Dus1"
                     /coded_by="XM_076690093.1:494..1909"
                     /db_xref="GeneID:117601822"
ORIGIN      
        1 mssvsaalae paniwetvlg spryivapmv daselawrll srrhgahlcy tpmlhssvfc
       61 rdpkyrreal astaedrpli vqfcgndpnt lleaallaep ycdavdinig cpqaiakrgr
      121 ygaflqddwd llrrivstls kglrvpvtck lrvfaeiekt veyarmlena garlltvhgr
      181 treqkgpltg laswdhikav rqavripvfa ngniqslqdv ekcmeetgvh gvmsaegnly
      241 npfifearyp psweaaleyl dlverypapa syirghlfkl fqhtlclaen keerenlarn
      301 ssmesfksvv ytlrdrylpy hegrlrwqqe tsdynlelpp wlcqpyarhl peecvrklet
      361 gkletqreet ikrkfkdddg neisrkrlkk lrriarrpnr ptvvikrgse lchdcpnpvg
      421 lkcvhklcrq ccrnkcften ldcighrnlt ktrrqmaief aakrktvhdq i
//
LOCUS       XP_034174968             470 aa            linear   INV 03-SEP-2025
DEFINITION  dihydrouridine synthase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034174968
VERSION     XP_034174968.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319077.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174968.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="dihydrouridine synthase 1 isoform X2"
                     /calculated_mol_wt=53860
     Region          23..246
                     /region_name="DUS_like_FMN"
                     /note="Dihydrouridine synthase-like (DUS-like) FMN-binding
                     domain. Members of this family catalyze the reduction of
                     the 5,6-double bond of a uridine residue on tRNA.
                     Dihydrouridine modification of tRNA is widely observed in
                     prokaryotes and eukaryotes, and also...; cd02801"
                     /db_xref="CDD:239200"
     Site            order(27..29,53,82,108,150,178,211,213,235..236)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239200"
     Site            order(82,111..112,150,152,177..178,180..181,212..213,236)
                     /site_type="active"
                     /db_xref="CDD:239200"
     Site            order(111,152,178,180)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239200"
     Site            order(112,150,177,181,212..213)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:239200"
     CDS             1..470
                     /gene="Dus1"
                     /coded_by="XM_034319077.2:220..1632"
                     /db_xref="GeneID:117601822"
ORIGIN      
        1 mssvsaalae paniwetvlg spryivapmv daselawrll srrhgahlcy tpmlhssvfc
       61 rdpkyrreal astaedrpli vqfcgndpnt lleaallaep ycdavdinig cpqaiakrgr
      121 ygaflqddwd llrrivstls kglrvpvtck lrvfaeiekt veyarmlena garlltvhgr
      181 treqkgpltg laswdhikav rqavripvfa ngniqslqdv ekcmeetgvh gvmsaegnly
      241 npfifearyp psweaaleyl dlverypapa syirghlfkl fqhtlclaen keerenlarn
      301 ssmesfksvv ytlrdrylpy hegrlrwqqe tsdynlelpp wlcqpyarhl peecvrklet
      361 gkletreeti krkfkdddgn eisrkrlkkl rriarrpnrp tvvikrgsel chdcpnpvgl
      421 kcvhklcrqc crnkcftenl dcighrnltk trrqmaiefa akrktvhdqi
//
LOCUS       XP_034175129             470 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S6 kinase beta-1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175129
VERSION     XP_034175129.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319238.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="ribosomal protein S6 kinase beta-1 isoform X1"
                     /calculated_mol_wt=52895
     Region          73..394
                     /region_name="STKc_p70S6K"
                     /note="Catalytic domain of the Serine/Threonine Kinase, 70
                     kDa ribosomal protein S6 kinase; cd05584"
                     /db_xref="CDD:270736"
     Site            order(75..79,83,99,101,134,150..151,153,157,159,196,198,
                     200..201,203,213..214,217,231..236,263,269,272)
                     /site_type="active"
                     /db_xref="CDD:270736"
     Site            order(75..79,83,99,101,134,151..153,157,196,198,200..201,
                     203,213..214)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270736"
     Site            order(79,157,159,196,198,200,217,231..236,263,269,272)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270736"
     Site            213..236
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270736"
     Site            372
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270736"
     Site            386..391
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270736"
     CDS             1..470
                     /gene="LOC117601895"
                     /coded_by="XM_034319238.2:351..1763"
                     /db_xref="GeneID:117601895"
ORIGIN      
        1 magvfdielh egdsvnqdes dddivesred aynhaanvnt ilesddlerv qlseqnvnig
       61 qektgpqdfe lckilgeggy gkvfqvkkvt gkdkggifam kvlrkasiir nqkdtahtka
      121 ernileavkh pfivnlmyaf qtggklylil eylcggelft yldregifle dtacfylsei
      181 ilalqhlhnq giiyrdlkpe nilldgeghv kltdfglcke hiqegtvtht fcgtieymap
      241 eiltrsghgk avdwwslgal mfdmltgmpp ftgddrrkti ekilrgklnl plyltpdakd
      301 lirrllkrqv lqrlgsgsed aeqimnhnff khinwqdvis kkleppfkps lksaddtsqf
      361 deqftttvpv dspvestlse sanmifqgft yvapsvleem yaqprvvnar sprkgllstg
      421 fsgslhnlps rspdahlrts shfhqyrhhv vgsnnmedte mkdigplfnf
//
LOCUS       XP_034175137             452 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S6 kinase beta-1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175137
VERSION     XP_034175137.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319246.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="ribosomal protein S6 kinase beta-1 isoform X2"
                     /calculated_mol_wt=50894
     Region          73..394
                     /region_name="STKc_p70S6K"
                     /note="Catalytic domain of the Serine/Threonine Kinase, 70
                     kDa ribosomal protein S6 kinase; cd05584"
                     /db_xref="CDD:270736"
     Site            order(75..79,83,99,101,134,150..151,153,157,159,196,198,
                     200..201,203,213..214,217,231..236,263,269,272)
                     /site_type="active"
                     /db_xref="CDD:270736"
     Site            order(75..79,83,99,101,134,151..153,157,196,198,200..201,
                     203,213..214)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270736"
     Site            order(79,157,159,196,198,200,217,231..236,263,269,272)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270736"
     Site            213..236
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270736"
     Site            372
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270736"
     Site            386..391
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270736"
     CDS             1..452
                     /gene="LOC117601895"
                     /coded_by="XM_034319246.2:353..1711"
                     /db_xref="GeneID:117601895"
ORIGIN      
        1 magvfdielh egdsvnqdes dddivesred aynhaanvnt ilesddlerv qlseqnvnig
       61 qektgpqdfe lckilgeggy gkvfqvkkvt gkdkggifam kvlrkasiir nqkdtahtka
      121 ernileavkh pfivnlmyaf qtggklylil eylcggelft yldregifle dtacfylsei
      181 ilalqhlhnq giiyrdlkpe nilldgeghv kltdfglcke hiqegtvtht fcgtieymap
      241 eiltrsghgk avdwwslgal mfdmltgmpp ftgddrrkti ekilrgklnl plyltpdakd
      301 lirrllkrqv lqrlgsgsed aeqimnhnff khinwqdvis kkleppfkps lksaddtsqf
      361 deqftttvpv dspvestlse sanmifqgft yvapsvleem yaqprvvnar sprkgllstg
      421 fsgslhnlps rspdahlrts shfhqyscff hf
//
LOCUS       XP_034175151             446 aa            linear   INV 03-SEP-2025
DEFINITION  corepressor interacting with RBPJ 1 [Osmia lignaria lignaria].
ACCESSION   XP_034175151
VERSION     XP_034175151.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319260.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175151.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="corepressor interacting with RBPJ 1"
                     /calculated_mol_wt=51428
     Region          14..49
                     /region_name="Cir_N"
                     /note="N-terminal domain of CBF1 interacting co-repressor
                     CIR; pfam10197"
                     /db_xref="CDD:462992"
     Region          <35..148
                     /region_name="TAF1"
                     /note="Transcription initiation factor TFIID, subunit TAF1
                     [Transcription]; COG5179"
                     /db_xref="CDD:227506"
     CDS             1..446
                     /gene="LOC117601906"
                     /coded_by="XM_034319260.2:138..1478"
                     /db_xref="GeneID:117601906"
ORIGIN      
        1 mgkgfnnymc kkffhpasrd nlkrvwmaeq qaeaykkkqe elrvqyekeq dlhnnkalls
       61 keskdklsvn fmyepppgak kerekednep eykfewqrky napresyckg dseirdqpfg
      121 iqvrnvrcik chkwghintd kecplysqam svvmannsqt psapdaltlv qqmredglal
      181 kksalsaqqh lrvpehehlm vsddeeqsei tflktlskte kkkllkklqq lekknkkvek
      241 qklkkhkvth ksrnyndkds nssystgins nskkrnrsss ssssssssss ssssnsssst
      301 stsssssvss sssssststs gtstsfdtdk hsvnnidnkt cngytsnksk krrnlekkkk
      361 kkkekadsvs ngnvkyrrkk eknkaksrkg enefkyerkn krkkshqrda kkrrtrdsft
      421 dkkrkisefn edknkdhesk kkirrf
//
LOCUS       XP_034175165             420 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome c oxidase assembly factor 10 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175165
VERSION     XP_034175165.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319274.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..420
                     /product="cytochrome c oxidase assembly factor 10 isoform
                     X1"
                     /calculated_mol_wt=46699
     Region          110..377
                     /region_name="PT_UbiA_Cox10"
                     /note="Protoheme IX farnesyltransferase; cd13957"
                     /db_xref="CDD:260120"
     CDS             1..420
                     /gene="Cox10"
                     /coded_by="XM_034319274.2:63..1325"
                     /db_xref="GeneID:117601910"
ORIGIN      
        1 mvfvlcssri srsiclsslk lsvtkyssea tstaqkltak iekhrktlpn tkslqgrtvs
       61 sdlidseddr rsvvsvasre deiekgpvsn dqrewryvel dsrklkrhcl mlskirltsl
      121 vvittmsgya lapgpfdvst fvvcsvgtgl vsatanainq ffevpfdaqm srtknrvlvr
      181 ghltpgqaif faavsgvtgl vllysqvngl taalgaanlv lytlvytpmk risilntwvg
      241 sivgaipplm gwaacvgdit spgawimsgl lyawqfphfn alswnlrpdy sragyrmmav
      301 tnpklcrtta lrytavltsl cylapvldvt nwwfaiastp lnayflylaw kfhkrsdsas
      361 srklfhfsli hlpvlmifml vnkkrwyndk kekggsmihe ekesnvyril tklitsctts
//
LOCUS       XP_034175171             395 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome c oxidase assembly factor 10 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175171
VERSION     XP_034175171.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319280.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..395
                     /product="cytochrome c oxidase assembly factor 10 isoform
                     X2"
                     /calculated_mol_wt=44022
     Region          85..352
                     /region_name="PT_UbiA_Cox10"
                     /note="Protoheme IX farnesyltransferase; cd13957"
                     /db_xref="CDD:260120"
     CDS             1..395
                     /gene="Cox10"
                     /coded_by="XM_034319280.2:127..1314"
                     /db_xref="GeneID:117601910"
ORIGIN      
        1 mnreatstaq kltakiekhr ktlpntkslq grtvssdlid seddrrsvvs vasredeiek
       61 gpvsndqrew ryveldsrkl krhclmlski rltslvvitt msgyalapgp fdvstfvvcs
      121 vgtglvsata nainqffevp fdaqmsrtkn rvlvrghltp gqaiffaavs gvtglvllys
      181 qvngltaalg aanlvlytlv ytpmkrisil ntwvgsivga ipplmgwaac vgditspgaw
      241 imsgllyawq fphfnalswn lrpdysragy rmmavtnpkl crttalryta vltslcylap
      301 vldvtnwwfa iastplnayf lylawkfhkr sdsassrklf hfslihlpvl mifmlvnkkr
      361 wyndkkekgg smiheekesn vyriltklit sctts
//
LOCUS       XP_034175180             373 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome c oxidase assembly factor 10 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175180
VERSION     XP_034175180.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319289.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175180.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="cytochrome c oxidase assembly factor 10 isoform
                     X3"
                     /calculated_mol_wt=40949
     Region          110..349
                     /region_name="PT_UbiA_Cox10"
                     /note="Protoheme IX farnesyltransferase; cd13957"
                     /db_xref="CDD:260120"
     CDS             1..373
                     /gene="Cox10"
                     /coded_by="XM_034319289.2:63..1184"
                     /db_xref="GeneID:117601910"
ORIGIN      
        1 mvfvlcssri srsiclsslk lsvtkyssea tstaqkltak iekhrktlpn tkslqgrtvs
       61 sdlidseddr rsvvsvasre deiekgpvsn dqrewryvel dsrklkrhcl mlskirltsl
      121 vvittmsgya lapgpfdvst fvvcsvgtgl vsatanainq ffevpfdaqm srtknrvlvr
      181 ghltpgqaif faavsgvtgl vllysqvngl taalgaanlv lytlvytpmk risilntwvg
      241 sivgaipplm gwaacvgdit spgawimsgl lyawqfphfn alswnlrpdy sragyrmmav
      301 tnpklcrtta lrytavltsl cylapvldvt nwwfaiastp lnayflylgt sihlgsfina
      361 pivqvranff tfr
//
LOCUS       XP_034175192             406 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein L38 [Osmia lignaria lignaria].
ACCESSION   XP_034175192
VERSION     XP_034175192.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319301.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="mitochondrial ribosomal protein L38"
                     /calculated_mol_wt=47601
     Region          141..292
                     /region_name="PEBP_euk"
                     /note="PhosphatidylEthanolamine-Binding Protein (PEBP)
                     domain present in eukaryotes; cd00866"
                     /db_xref="CDD:176644"
     Site            order(190,202,204,227..230,236,238)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176644"
     CDS             1..406
                     /gene="mRpL38"
                     /coded_by="XM_034319301.2:356..1576"
                     /db_xref="GeneID:117601927"
ORIGIN      
        1 mantvfrift rdvsprltir meqvrcghhl rgkpptiarn lkqrleelnq tdpalsfkvn
       61 igfsvpkiss ekksiwmnei kvnrsddqfe kqlrsgelpv nletvkevww easspfhirt
      121 iadhygifqd tfgdayffpv vpleiaykve edafakvytg nvikpaeask ppaiaykaes
      181 dslwtlimst pdgnmqnssn eychwflgni penkigqgee iidylrpitp rgvgyyryaf
      241 ilykqnqrld yaeykktppp clrltdrdwn tlkfyqkyqd yltpaglaff qsdwdptvtk
      301 fyhsvlgtke pifqydfpkq yikpqvwfpk rqpfnlymdr ykdpkdvmke fllrklkivh
      361 pfkepkpplk ypnactfprd tpswlklekt kerlgrgrvn emnesv
//
LOCUS       XP_034175211             399 aa            linear   INV 03-SEP-2025
DEFINITION  C45 family peptidase tan [Osmia lignaria lignaria].
ACCESSION   XP_034175211
VERSION     XP_034175211.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319320.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175211.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="C45 family peptidase tan"
                     /calculated_mol_wt=44499
     Region          29..369
                     /region_name="C45_proenzyme"
                     /note="C45 family autoproteolytic
                     acyltransferase/hydolase; NF040521"
                     /db_xref="CDD:468523"
     CDS             1..399
                     /gene="tan"
                     /coded_by="XM_034319320.2:251..1450"
                     /db_xref="GeneID:117601935"
ORIGIN      
        1 mttpddsavc spqtkdigrr lavpiihtrg thydigfdig rtfsgliqey vdtyrpvnet
       61 ylplyetetg rkiyeetlgc veeqfpgylr eirgtadgan vpfhklflmh lddivpnvan
      121 egvesnalpv gcstivcnqp gqeilghned algetlnhwy lvaghviepg ckeehftsls
      181 yagflpgytm gynhhglvyt vntlsaaklr sgktpryflt rallaaenyv qaqqilrneg
      241 fgaaegfsvn mtfltqqgdr mfhnaeigpc dldaaqsqln iltvspgent fhcnkylrlk
      301 ipevkgliid ssvrrmdtic khpppksrqd vinilsdqtd neyrvyqeik kddyvktiat
      361 gifdciertw siytdkpnct epvlviplql cdeptiaav
//
LOCUS       XP_076546210             385 aa            linear   INV 03-SEP-2025
DEFINITION  arf-GAP with dual PH domain-containing protein 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546210
VERSION     XP_076546210.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690095.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..385
                     /product="arf-GAP with dual PH domain-containing protein 1
                     isoform X1"
                     /calculated_mol_wt=44188
     Region          2..114
                     /region_name="ArfGap_ADAP"
                     /note="ArfGap with dual PH domains; cd08832"
                     /db_xref="CDD:350061"
     Site            order(21,24,41,44,49)
                     /site_type="other"
                     /note="arginine finger"
                     /db_xref="CDD:350061"
     Site            order(21,24,41,44)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:350061"
     Region          131..238
                     /region_name="PH1_ADAP"
                     /note="ArfGAP with dual PH domains Pleckstrin homology
                     (PH) domain, repeat 1; cd13252"
                     /db_xref="CDD:270072"
     Site            order(138,144,146..148,186..193,211..214,216..217)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:270072"
     Site            order(138,144,146..148,186..193,211,213)
                     /site_type="other"
                     /note="KIF12B binding site [polypeptide binding]"
                     /db_xref="CDD:270072"
     Site            order(139,142..144,150,163,165,172,206)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270072"
     Region          252..355
                     /region_name="PH2_ADAP"
                     /note="ArfGAP with dual PH domains Pleckstrin homology
                     (PH) domain, repeat 2; cd01251"
                     /db_xref="CDD:241282"
     Site            order(261,269,271,273,284,294,330)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:241282"
     CDS             1..385
                     /gene="LOC143305658"
                     /coded_by="XM_076690095.1:217..1374"
                     /db_xref="GeneID:143305658"
ORIGIN      
        1 madlneklla ellkkpgnnv cadcgaknpe wasynigifv ctrcagihrs mgahiskvkh
       61 lkldrwedsq vnrirevgnv aarlhyeerv ppcyrrpspd apqvlveqwi rakyereefc
      121 hperqnhyvs gfmegflmkr gkedsryhpr kfvlceaedt lkyhvkenke pkavlrisel
      181 nvafappktg nqnslqisfm kdgttrhiyv yhedpevitn wylaircakl hrlqvaypga
      241 teaellsqlt rdfpregflw ktgprhtday kkrwftldgr klmyhddpmd ahpkgeiflg
      301 hssdgfavkt gvppgardqg fsftletpdr tyllstqsdd drtqwmniiq kvidkpltpq
      361 datvaarlir krtasgtmni fsstr
//
LOCUS       XP_076546211             335 aa            linear   INV 03-SEP-2025
DEFINITION  arf-GAP with dual PH domain-containing protein 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546211
VERSION     XP_076546211.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690096.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..335
                     /product="arf-GAP with dual PH domain-containing protein 1
                     isoform X2"
                     /calculated_mol_wt=38769
     Region          <1..64
                     /region_name="ArfGap"
                     /note="GTPase-activating protein (GAP) for the ADP
                     ribosylation factors (ARFs); cl28907"
                     /db_xref="CDD:355783"
     Region          81..188
                     /region_name="PH1_ADAP"
                     /note="ArfGAP with dual PH domains Pleckstrin homology
                     (PH) domain, repeat 1; cd13252"
                     /db_xref="CDD:270072"
     Site            order(88,94,96..98,136..143,161..164,166..167)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:270072"
     Site            order(88,94,96..98,136..143,161,163)
                     /site_type="other"
                     /note="KIF12B binding site [polypeptide binding]"
                     /db_xref="CDD:270072"
     Site            order(89,92..94,100,113,115,122,156)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270072"
     Region          202..305
                     /region_name="PH2_ADAP"
                     /note="ArfGAP with dual PH domains Pleckstrin homology
                     (PH) domain, repeat 2; cd01251"
                     /db_xref="CDD:241282"
     Site            order(211,219,221,223,234,244,280)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:241282"
     CDS             1..335
                     /gene="LOC143305658"
                     /coded_by="XM_076690096.1:160..1167"
                     /db_xref="GeneID:143305658"
ORIGIN      
        1 mgahiskvkh lkldrwedsq vnrirevgnv aarlhyeerv ppcyrrpspd apqvlveqwi
       61 rakyereefc hperqnhyvs gfmegflmkr gkedsryhpr kfvlceaedt lkyhvkenke
      121 pkavlrisel nvafappktg nqnslqisfm kdgttrhiyv yhedpevitn wylaircakl
      181 hrlqvaypga teaellsqlt rdfpregflw ktgprhtday kkrwftldgr klmyhddpmd
      241 ahpkgeiflg hssdgfavkt gvppgardqg fsftletpdr tyllstqsdd drtqwmniiq
      301 kvidkpltpq datvaarlir krtasgtmni fsstr
//
LOCUS       XP_076546212             335 aa            linear   INV 03-SEP-2025
DEFINITION  arf-GAP with dual PH domain-containing protein 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546212
VERSION     XP_076546212.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690097.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..335
                     /product="arf-GAP with dual PH domain-containing protein 1
                     isoform X2"
                     /calculated_mol_wt=38769
     Region          <1..64
                     /region_name="ArfGap"
                     /note="GTPase-activating protein (GAP) for the ADP
                     ribosylation factors (ARFs); cl28907"
                     /db_xref="CDD:355783"
     Region          81..188
                     /region_name="PH1_ADAP"
                     /note="ArfGAP with dual PH domains Pleckstrin homology
                     (PH) domain, repeat 1; cd13252"
                     /db_xref="CDD:270072"
     Site            order(88,94,96..98,136..143,161..164,166..167)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:270072"
     Site            order(88,94,96..98,136..143,161,163)
                     /site_type="other"
                     /note="KIF12B binding site [polypeptide binding]"
                     /db_xref="CDD:270072"
     Site            order(89,92..94,100,113,115,122,156)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270072"
     Region          202..305
                     /region_name="PH2_ADAP"
                     /note="ArfGAP with dual PH domains Pleckstrin homology
                     (PH) domain, repeat 2; cd01251"
                     /db_xref="CDD:241282"
     Site            order(211,219,221,223,234,244,280)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:241282"
     CDS             1..335
                     /gene="LOC143305658"
                     /coded_by="XM_076690097.1:371..1378"
                     /db_xref="GeneID:143305658"
ORIGIN      
        1 mgahiskvkh lkldrwedsq vnrirevgnv aarlhyeerv ppcyrrpspd apqvlveqwi
       61 rakyereefc hperqnhyvs gfmegflmkr gkedsryhpr kfvlceaedt lkyhvkenke
      121 pkavlrisel nvafappktg nqnslqisfm kdgttrhiyv yhedpevitn wylaircakl
      181 hrlqvaypga teaellsqlt rdfpregflw ktgprhtday kkrwftldgr klmyhddpmd
      241 ahpkgeiflg hssdgfavkt gvppgardqg fsftletpdr tyllstqsdd drtqwmniiq
      301 kvidkpltpq datvaarlir krtasgtmni fsstr
//
LOCUS       XP_076546213             378 aa            linear   INV 03-SEP-2025
DEFINITION  exopolyphosphatase PRUNE1-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546213
VERSION     XP_076546213.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690098.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..378
                     /product="exopolyphosphatase PRUNE1-like isoform X1"
                     /calculated_mol_wt=42287
     Region          23..>249
                     /region_name="PPX1"
                     /note="Inorganic pyrophosphatase/exopolyphosphatase
                     [Energy production and conversion]; COG1227"
                     /db_xref="CDD:440840"
     Region          228..369
                     /region_name="DHHA2"
                     /note="DHHA2 domain; pfam02833"
                     /db_xref="CDD:460719"
     CDS             1..378
                     /gene="LOC143305659"
                     /coded_by="XM_076690098.1:435..1571"
                     /db_xref="GeneID:143305659"
ORIGIN      
        1 mepflltskt alsnlsayek iriilgngtc dldsavsala qgffdyldgm kndatklavi
       61 pvmniperey rvktevvffl krhnipftll tfrdeidlkr lkekkgitle ivlvdhhqlp
      121 eedafladfv vkvidhrpqd anwlwpgrtv qlervgscat lvarnlldry peamdtqlfs
      181 llrgpilidt cnfskeadra tsadvevahl letlgqldld rskafdeive aksdiskltv
      241 ddflirdlkv angvpivglp ilvekflelq dvfgaiknfs ksrnatvivf igleirsrql
      301 hrdiavfsfl avnplkdqiv kaltsstqps lqltliketn qvngngslll yaqgnlrvtr
      361 kqilplvrdt vtsancrc
//
LOCUS       XP_076546214             378 aa            linear   INV 03-SEP-2025
DEFINITION  exopolyphosphatase PRUNE1-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546214
VERSION     XP_076546214.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690099.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..378
                     /product="exopolyphosphatase PRUNE1-like isoform X1"
                     /calculated_mol_wt=42287
     Region          23..>249
                     /region_name="PPX1"
                     /note="Inorganic pyrophosphatase/exopolyphosphatase
                     [Energy production and conversion]; COG1227"
                     /db_xref="CDD:440840"
     Region          228..369
                     /region_name="DHHA2"
                     /note="DHHA2 domain; pfam02833"
                     /db_xref="CDD:460719"
     CDS             1..378
                     /gene="LOC143305659"
                     /coded_by="XM_076690099.1:439..1575"
                     /db_xref="GeneID:143305659"
ORIGIN      
        1 mepflltskt alsnlsayek iriilgngtc dldsavsala qgffdyldgm kndatklavi
       61 pvmniperey rvktevvffl krhnipftll tfrdeidlkr lkekkgitle ivlvdhhqlp
      121 eedafladfv vkvidhrpqd anwlwpgrtv qlervgscat lvarnlldry peamdtqlfs
      181 llrgpilidt cnfskeadra tsadvevahl letlgqldld rskafdeive aksdiskltv
      241 ddflirdlkv angvpivglp ilvekflelq dvfgaiknfs ksrnatvivf igleirsrql
      301 hrdiavfsfl avnplkdqiv kaltsstqps lqltliketn qvngngslll yaqgnlrvtr
      361 kqilplvrdt vtsancrc
//
LOCUS       XP_076546215             329 aa            linear   INV 03-SEP-2025
DEFINITION  exopolyphosphatase PRUNE1-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546215
VERSION     XP_076546215.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690100.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="exopolyphosphatase PRUNE1-like isoform X2"
                     /calculated_mol_wt=37034
     Region          51..>200
                     /region_name="PPX1"
                     /note="Inorganic pyrophosphatase/exopolyphosphatase
                     [Energy production and conversion]; COG1227"
                     /db_xref="CDD:440840"
     Region          179..320
                     /region_name="DHHA2"
                     /note="DHHA2 domain; pfam02833"
                     /db_xref="CDD:460719"
     CDS             1..329
                     /gene="LOC143305659"
                     /coded_by="XM_076690100.1:267..1256"
                     /db_xref="GeneID:143305659"
ORIGIN      
        1 mkndatklav ipvmnipere yrvktevvff lkrhnipftl ltfrdeidlk rlkekkgitl
       61 eivlvdhhql peedafladf vvkvidhrpq danwlwpgrt vqlervgsca tlvarnlldr
      121 ypeamdtqlf sllrgpilid tcnfskeadr atsadvevah lletlgqldl drskafdeiv
      181 eaksdisklt vddflirdlk vangvpivgl pilvekflel qdvfgaiknf sksrnatviv
      241 figleirsrq lhrdiavfsf lavnplkdqi vkaltsstqp slqltliket nqvngngsll
      301 lyaqgnlrvt rkqilplvrd tvtsancrc
//
LOCUS       XP_076546217             329 aa            linear   INV 03-SEP-2025
DEFINITION  exopolyphosphatase PRUNE1-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546217
VERSION     XP_076546217.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690102.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="exopolyphosphatase PRUNE1-like isoform X2"
                     /calculated_mol_wt=37034
     Region          51..>200
                     /region_name="PPX1"
                     /note="Inorganic pyrophosphatase/exopolyphosphatase
                     [Energy production and conversion]; COG1227"
                     /db_xref="CDD:440840"
     Region          179..320
                     /region_name="DHHA2"
                     /note="DHHA2 domain; pfam02833"
                     /db_xref="CDD:460719"
     CDS             1..329
                     /gene="LOC143305659"
                     /coded_by="XM_076690102.1:475..1464"
                     /db_xref="GeneID:143305659"
ORIGIN      
        1 mkndatklav ipvmnipere yrvktevvff lkrhnipftl ltfrdeidlk rlkekkgitl
       61 eivlvdhhql peedafladf vvkvidhrpq danwlwpgrt vqlervgsca tlvarnlldr
      121 ypeamdtqlf sllrgpilid tcnfskeadr atsadvevah lletlgqldl drskafdeiv
      181 eaksdisklt vddflirdlk vangvpivgl pilvekflel qdvfgaiknf sksrnatviv
      241 figleirsrq lhrdiavfsf lavnplkdqi vkaltsstqp slqltliket nqvngngsll
      301 lyaqgnlrvt rkqilplvrd tvtsancrc
//
LOCUS       XP_034175221             364 aa            linear   INV 03-SEP-2025
DEFINITION  putative E3 ubiquitin-protein ligase UBR7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175221
VERSION     XP_034175221.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319330.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="putative E3 ubiquitin-protein ligase UBR7"
                     /calculated_mol_wt=42229
     Region          42..110
                     /region_name="UBR-box_UBR7"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR7 and similar proteins; cd19677"
                     /db_xref="CDD:439075"
     Site            order(57,60,70,73,77..78,81,94,96,106)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439075"
     Region          129..182
                     /region_name="PHD_UBR7"
                     /note="PHD finger found in putative E3 ubiquitin-protein
                     ligase UBR7; cd15542"
                     /db_xref="CDD:277017"
     Site            order(129,146..150,154,177)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277017"
     CDS             1..364
                     /gene="LOC117601952"
                     /coded_by="XM_034319330.2:586..1680"
                     /db_xref="GeneID:117601952"
ORIGIN      
        1 msenieeiie eessvtmldv lreenqleed acavlgasdd knctyskgyt rqalyacksc
       61 cpksvraavc lactfhcheg helielytkr hfrcdcgnsk faenkcnldp skdpvnsenr
      121 ynhnfdglyc ickrpypdpe dtvndemlqc iicedwyhsk hlecekgipa ddaydemicg
      181 kcmkennflw nyaskyavfe ttekekekek ekkeeaieie kqpegctmpr knfskepltk
      241 gtcfwtegwr svlcvcdtck elyrqknvtf lldptdsvha yeeagkinnq esqyekgmka
      301 laslgrveql taieeynnmk erlkqylqkf aenkkvvree dikeffseme skkrpkvvvp
      361 tycr
//
LOCUS       XP_034175227             364 aa            linear   INV 03-SEP-2025
DEFINITION  putative E3 ubiquitin-protein ligase UBR7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175227
VERSION     XP_034175227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319336.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="putative E3 ubiquitin-protein ligase UBR7"
                     /calculated_mol_wt=42229
     Region          42..110
                     /region_name="UBR-box_UBR7"
                     /note="UBR-box found in RING-type E3 ubiquitin-protein
                     ligase UBR7 and similar proteins; cd19677"
                     /db_xref="CDD:439075"
     Site            order(57,60,70,73,77..78,81,94,96,106)
                     /site_type="other"
                     /note="putative Zn binding site [ion binding]"
                     /db_xref="CDD:439075"
     Region          129..182
                     /region_name="PHD_UBR7"
                     /note="PHD finger found in putative E3 ubiquitin-protein
                     ligase UBR7; cd15542"
                     /db_xref="CDD:277017"
     Site            order(129,146..150,154,177)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277017"
     CDS             1..364
                     /gene="LOC117601952"
                     /coded_by="XM_034319336.2:259..1353"
                     /db_xref="GeneID:117601952"
ORIGIN      
        1 msenieeiie eessvtmldv lreenqleed acavlgasdd knctyskgyt rqalyacksc
       61 cpksvraavc lactfhcheg helielytkr hfrcdcgnsk faenkcnldp skdpvnsenr
      121 ynhnfdglyc ickrpypdpe dtvndemlqc iicedwyhsk hlecekgipa ddaydemicg
      181 kcmkennflw nyaskyavfe ttekekekek ekkeeaieie kqpegctmpr knfskepltk
      241 gtcfwtegwr svlcvcdtck elyrqknvtf lldptdsvha yeeagkinnq esqyekgmka
      301 laslgrveql taieeynnmk erlkqylqkf aenkkvvree dikeffseme skkrpkvvvp
      361 tycr
//
LOCUS       XP_076546218             326 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-sugar transporter UST74c isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546218
VERSION     XP_076546218.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690103.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="UDP-sugar transporter UST74c isoform X1"
                     /calculated_mol_wt=36397
     Region          15..298
                     /region_name="VRG4"
                     /note="Nucleotide-sugar transporter [Carbohydrate
                     transport and metabolism / Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5070"
                     /db_xref="CDD:227402"
     CDS             1..326
                     /gene="LOC117601968"
                     /coded_by="XM_076690103.1:608..1588"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mslkdqnvmf arvgsalfyg lssfmitvvn ktvltsfefp sfqvlgigqm lativllfaa
       61 kklryvefpn leattfvkiw plpviyignm ifglggtkql slpmftalrr fsilmtmiae
      121 yyvlgarprv svqlsvytmi igaivaalnd lafsfkgyvf illndfftav ngvymkkkld
      181 nkelgkyglm yynslfmivp aivlacsmgd arsvlefpyw cnvfflvqfg vscvmgfvls
      241 ysmilctlyn saltttiigc lknicvtylg mviggdyift wlnflglnls vvgslvytwv
      301 tfrrrepvep kysplvedqa skvlav
//
LOCUS       XP_076546219             326 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-sugar transporter UST74c isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546219
VERSION     XP_076546219.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690104.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="UDP-sugar transporter UST74c isoform X1"
                     /calculated_mol_wt=36397
     Region          15..298
                     /region_name="VRG4"
                     /note="Nucleotide-sugar transporter [Carbohydrate
                     transport and metabolism / Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5070"
                     /db_xref="CDD:227402"
     CDS             1..326
                     /gene="LOC117601968"
                     /coded_by="XM_076690104.1:641..1621"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mslkdqnvmf arvgsalfyg lssfmitvvn ktvltsfefp sfqvlgigqm lativllfaa
       61 kklryvefpn leattfvkiw plpviyignm ifglggtkql slpmftalrr fsilmtmiae
      121 yyvlgarprv svqlsvytmi igaivaalnd lafsfkgyvf illndfftav ngvymkkkld
      181 nkelgkyglm yynslfmivp aivlacsmgd arsvlefpyw cnvfflvqfg vscvmgfvls
      241 ysmilctlyn saltttiigc lknicvtylg mviggdyift wlnflglnls vvgslvytwv
      301 tfrrrepvep kysplvedqa skvlav
//
LOCUS       XP_076546220             326 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-sugar transporter UST74c isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546220
VERSION     XP_076546220.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690105.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="UDP-sugar transporter UST74c isoform X1"
                     /calculated_mol_wt=36397
     Region          15..298
                     /region_name="VRG4"
                     /note="Nucleotide-sugar transporter [Carbohydrate
                     transport and metabolism / Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5070"
                     /db_xref="CDD:227402"
     CDS             1..326
                     /gene="LOC117601968"
                     /coded_by="XM_076690105.1:611..1591"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mslkdqnvmf arvgsalfyg lssfmitvvn ktvltsfefp sfqvlgigqm lativllfaa
       61 kklryvefpn leattfvkiw plpviyignm ifglggtkql slpmftalrr fsilmtmiae
      121 yyvlgarprv svqlsvytmi igaivaalnd lafsfkgyvf illndfftav ngvymkkkld
      181 nkelgkyglm yynslfmivp aivlacsmgd arsvlefpyw cnvfflvqfg vscvmgfvls
      241 ysmilctlyn saltttiigc lknicvtylg mviggdyift wlnflglnls vvgslvytwv
      301 tfrrrepvep kysplvedqa skvlav
//
LOCUS       XP_076546221             326 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-sugar transporter UST74c isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546221
VERSION     XP_076546221.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690106.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="UDP-sugar transporter UST74c isoform X1"
                     /calculated_mol_wt=36397
     Region          15..298
                     /region_name="VRG4"
                     /note="Nucleotide-sugar transporter [Carbohydrate
                     transport and metabolism / Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5070"
                     /db_xref="CDD:227402"
     CDS             1..326
                     /gene="LOC117601968"
                     /coded_by="XM_076690106.1:650..1630"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mslkdqnvmf arvgsalfyg lssfmitvvn ktvltsfefp sfqvlgigqm lativllfaa
       61 kklryvefpn leattfvkiw plpviyignm ifglggtkql slpmftalrr fsilmtmiae
      121 yyvlgarprv svqlsvytmi igaivaalnd lafsfkgyvf illndfftav ngvymkkkld
      181 nkelgkyglm yynslfmivp aivlacsmgd arsvlefpyw cnvfflvqfg vscvmgfvls
      241 ysmilctlyn saltttiigc lknicvtylg mviggdyift wlnflglnls vvgslvytwv
      301 tfrrrepvep kysplvedqa skvlav
//
LOCUS       XP_076546222             326 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-sugar transporter UST74c isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546222
VERSION     XP_076546222.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690107.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="UDP-sugar transporter UST74c isoform X1"
                     /calculated_mol_wt=36397
     Region          15..298
                     /region_name="VRG4"
                     /note="Nucleotide-sugar transporter [Carbohydrate
                     transport and metabolism / Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5070"
                     /db_xref="CDD:227402"
     CDS             1..326
                     /gene="LOC117601968"
                     /coded_by="XM_076690107.1:642..1622"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mslkdqnvmf arvgsalfyg lssfmitvvn ktvltsfefp sfqvlgigqm lativllfaa
       61 kklryvefpn leattfvkiw plpviyignm ifglggtkql slpmftalrr fsilmtmiae
      121 yyvlgarprv svqlsvytmi igaivaalnd lafsfkgyvf illndfftav ngvymkkkld
      181 nkelgkyglm yynslfmivp aivlacsmgd arsvlefpyw cnvfflvqfg vscvmgfvls
      241 ysmilctlyn saltttiigc lknicvtylg mviggdyift wlnflglnls vvgslvytwv
      301 tfrrrepvep kysplvedqa skvlav
//
LOCUS       XP_076546223             326 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-sugar transporter UST74c isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546223
VERSION     XP_076546223.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690108.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="UDP-sugar transporter UST74c isoform X1"
                     /calculated_mol_wt=36397
     Region          15..298
                     /region_name="VRG4"
                     /note="Nucleotide-sugar transporter [Carbohydrate
                     transport and metabolism / Posttranslational modification,
                     protein turnover, chaperones / Intracellular trafficking
                     and secretion]; COG5070"
                     /db_xref="CDD:227402"
     CDS             1..326
                     /gene="LOC117601968"
                     /coded_by="XM_076690108.1:631..1611"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mslkdqnvmf arvgsalfyg lssfmitvvn ktvltsfefp sfqvlgigqm lativllfaa
       61 kklryvefpn leattfvkiw plpviyignm ifglggtkql slpmftalrr fsilmtmiae
      121 yyvlgarprv svqlsvytmi igaivaalnd lafsfkgyvf illndfftav ngvymkkkld
      181 nkelgkyglm yynslfmivp aivlacsmgd arsvlefpyw cnvfflvqfg vscvmgfvls
      241 ysmilctlyn saltttiigc lknicvtylg mviggdyift wlnflglnls vvgslvytwv
      301 tfrrrepvep kysplvedqa skvlav
//
LOCUS       XP_034175250             253 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquinone biosynthesis protein COQ4 homolog, mitochondrial isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034175250
VERSION     XP_034175250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319359.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="ubiquinone biosynthesis protein COQ4 homolog,
                     mitochondrial isoform X2"
                     /calculated_mol_wt=28844
     Region          36..247
                     /region_name="Coq4"
                     /note="Coenzyme Q (ubiquinone) biosynthesis protein Coq4;
                     pfam05019"
                     /db_xref="CDD:461522"
     CDS             1..253
                     /gene="LOC117601968"
                     /coded_by="XM_034319359.2:546..1307"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mirlyqcrpi rrlfsdasfa kdfakdyakh rvslspmqra iltagaaais fanpfrgdmi
       61 aclgettgtd alsyclrqml ataegrrild ekprisssti dlsalkrlpp gtvgrtyrdf
      121 ldannvtpds rpsvrfvedt elayvmqryr emhdifhavl lmpttmlgev svkwiealql
      181 rlpmclsgai fgayrlrprq rklylehhlp waiktgsrak fllgiyyeer weqplvdfhr
      241 etnvtplvpi gyn
//
LOCUS       XP_076546224             336 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquinone biosynthesis protein COQ4 homolog, mitochondrial isoform
            X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546224
VERSION     XP_076546224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690109.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="ubiquinone biosynthesis protein COQ4 homolog,
                     mitochondrial isoform X3"
                     /calculated_mol_wt=37974
     Region          119..330
                     /region_name="Coq4"
                     /note="Coenzyme Q (ubiquinone) biosynthesis protein Coq4;
                     pfam05019"
                     /db_xref="CDD:461522"
     CDS             1..336
                     /gene="LOC117601968"
                     /coded_by="XM_076690109.1:704..1714"
                     /db_xref="GeneID:117601968"
ORIGIN      
        1 mslkdqnvmf arvgsalfyg lssfmitvvn ktvltsfefp sfqvlgigqm lativllfaa
       61 kklryvefpn leattfvkiw plpviyivsg rpirrlfsda sfakdfakdy akhrvslspm
      121 qrailtagaa aisfanpfrg dmiaclgett gtdalsyclr qmlataegrr ildekpriss
      181 stidlsalkr lppgtvgrty rdfldannvt pdsrpsvrfv edtelayvmq ryremhdifh
      241 avllmpttml gevsvkwiea lqlrlpmcls gaifgayrlr prqrklyleh hlpwaiktgs
      301 rakfllgiyy eerweqplvd fhretnvtpl vpigyn
//
LOCUS       XP_034175286             233 aa            linear   INV 03-SEP-2025
DEFINITION  mpv17-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034175286
VERSION     XP_034175286.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319395.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175286.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="mpv17-like protein"
                     /calculated_mol_wt=26949
     Region          154..213
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..233
                     /gene="LOC117601993"
                     /coded_by="XM_034319395.2:3..704"
                     /db_xref="GeneID:117601993"
ORIGIN      
        1 msvlrilqkl khsaqkrpll fnsviygsfy tgaeftrqty srifkfplss msentvelnt
       61 ienkrplfiw tnkfnetlgl ldenstglrd ynwaqlkrya iygcfiagpv lhawykwlda
      121 fykgtamkii laklcvdqfv ltppliiiff ismslmegkq difneckakf lqtfktscmy
      181 wlpvqflnfl lipptlrvsf vgvaafcwvn ilcylksspl idsnnnnnse iky
//
LOCUS       XP_034175298             228 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoserine phosphatase [Osmia lignaria lignaria].
ACCESSION   XP_034175298
VERSION     XP_034175298.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319407.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="phosphoserine phosphatase"
                     /calculated_mol_wt=25164
     Region          4..224
                     /region_name="PLN02954"
                     /note="phosphoserine phosphatase"
                     /db_xref="CDD:215514"
     Site            order(20..24,29,109..110,158,178..179,182..183)
                     /site_type="active"
                     /db_xref="CDD:319801"
     CDS             1..228
                     /gene="aay"
                     /coded_by="XM_034319407.2:176..862"
                     /db_xref="GeneID:117602000"
ORIGIN      
        1 mahseemksi wrsanavtfd vdstviqeeg idelakfcgk ekevaaltnq amqgnvtfrq
       61 slverlniik pnlmqinrfl ashplklspg iktlitalqi hkkqvflvsg gfhcliapva
      121 sslnipleni fanrlkfyft geyagfdedq ptaenggkak virylkegkg fqtivhigdg
      181 atdlettpiv dlfigyggnv iresvkskss wfitnfdelv niletqtv
//
LOCUS       XP_034175307             228 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoserine phosphatase [Osmia lignaria lignaria].
ACCESSION   XP_034175307
VERSION     XP_034175307.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319416.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="phosphoserine phosphatase"
                     /calculated_mol_wt=25164
     Region          4..224
                     /region_name="PLN02954"
                     /note="phosphoserine phosphatase"
                     /db_xref="CDD:215514"
     Site            order(20..24,29,109..110,158,178..179,182..183)
                     /site_type="active"
                     /db_xref="CDD:319801"
     CDS             1..228
                     /gene="aay"
                     /coded_by="XM_034319416.2:144..830"
                     /db_xref="GeneID:117602000"
ORIGIN      
        1 mahseemksi wrsanavtfd vdstviqeeg idelakfcgk ekevaaltnq amqgnvtfrq
       61 slverlniik pnlmqinrfl ashplklspg iktlitalqi hkkqvflvsg gfhcliapva
      121 sslnipleni fanrlkfyft geyagfdedq ptaenggkak virylkegkg fqtivhigdg
      181 atdlettpiv dlfigyggnv iresvkskss wfitnfdelv niletqtv
//
LOCUS       XP_034175321             218 aa            linear   INV 03-SEP-2025
DEFINITION  sesquipedalian-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034175321
VERSION     XP_034175321.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319430.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..218
                     /product="sesquipedalian-1 isoform X1"
                     /calculated_mol_wt=25176
     Region          11..132
                     /region_name="PH_Ses"
                     /note="Sesquipedalian family Pleckstrin homology (PH)
                     domain; cd13288"
                     /db_xref="CDD:270105"
     CDS             1..218
                     /gene="LOC117602008"
                     /coded_by="XM_034319430.2:331..987"
                     /db_xref="GeneID:117602008"
ORIGIN      
        1 mkineknmva fatsatpvdr egwlnkrgel nrgyqrrwfv lkgnilfyfd rrgdkepvgm
       61 ivlegctiel aedeeqfgfq ivfhgpnnrn yalaaesqvk esmeqwmkal acasydymkl
      121 mvaelqrqld aaeeettmvt apsqspkapp rqrhnpfnrs emhhrsqsvr sapgrtenip
      181 rtkitfrelh tmygrrilad lnewrhtrka aeaplisf
//
LOCUS       XP_034175329             216 aa            linear   INV 03-SEP-2025
DEFINITION  sesquipedalian-1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034175329
VERSION     XP_034175329.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319438.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..216
                     /product="sesquipedalian-1 isoform X2"
                     /calculated_mol_wt=24948
     Region          11..130
                     /region_name="PH_Ses"
                     /note="Sesquipedalian family Pleckstrin homology (PH)
                     domain; cd13288"
                     /db_xref="CDD:270105"
     CDS             1..216
                     /gene="LOC117602008"
                     /coded_by="XM_034319438.2:291..941"
                     /db_xref="GeneID:117602008"
ORIGIN      
        1 mkineknmva fatsatpvdr egwlnkrgel nrgyqrrwfv lkgnilfyfd rrgdkepvgm
       61 ivlegctiel aedeeqfgfq ivfhgpnnrn yalaaesqes meqwmkalac asydymklmv
      121 aelqrqldaa eeettmvtap sqspkapprq rhnpfnrsem hhrsqsvrsa pgrteniprt
      181 kitfrelhtm ygrriladln ewrhtrkaae aplisf
//
LOCUS       XP_034175342             199 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 9-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034175342
VERSION     XP_034175342.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319451.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="N-acetyltransferase 9-like protein"
                     /calculated_mol_wt=23765
     Region          14..158
                     /region_name="Acetyltransf_3"
                     /note="Acetyltransferase (GNAT) domain; pfam13302"
                     /db_xref="CDD:379112"
     CDS             1..199
                     /gene="LOC117602019"
                     /coded_by="XM_034319451.2:64..663"
                     /db_xref="GeneID:117602019"
ORIGIN      
        1 mkdnehikii gtnvilipyk ekhvkryhew mkseelryft gsevltleee femqkrwhqd
       61 qdkctfiile ksifsatane ieamigdtnl ffnqsdefns aeveimianv acrgkkrgwe
      121 amilmllygi eklnitkyma kikfnneksl nmfeklkfyk vekshifqey tfekvvdqqw
      181 kdwlhsnvkd tiiyetydg
//
LOCUS       XP_034174708            2110 aa            linear   INV 03-SEP-2025
DEFINITION  bridge-like lipid transfer protein family member hobbit [Osmia
            lignaria lignaria].
ACCESSION   XP_034174708
VERSION     XP_034174708.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318817.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174708.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2110
                     /product="bridge-like lipid transfer protein family member
                     hobbit"
                     /calculated_mol_wt=238212
     Region          22..>145
                     /region_name="Fmp27"
                     /note="Mitochondrial protein from FMP27; pfam10344"
                     /db_xref="CDD:431223"
     Region          724..843
                     /region_name="DUF2405"
                     /note="Domain of unknown function (DUF2405); pfam10293"
                     /db_xref="CDD:431201"
     Region          952..1084
                     /region_name="Fmp27_GFWDK"
                     /note="RNA pol II promoter Fmp27 protein domain;
                     pfam10347"
                     /db_xref="CDD:402113"
     Region          1635..2064
                     /region_name="Apt1"
                     /note="Golgi-body localization protein domain; pfam10351"
                     /db_xref="CDD:463055"
     CDS             1..2110
                     /gene="hob"
                     /coded_by="XM_034318817.2:169..6501"
                     /db_xref="GeneID:117601689"
ORIGIN      
        1 mtglfafcai cfvlycnftw viprfiawlv krrykiylrv ghislpyfil rdvniskngf
       61 tlqieeisir sslfssdvak llavimkdvr vnkdvpvksv rvakrrtklm dfrnkrippi
      121 iitfvqfmav hvenlsllil gngwlangsa esvsfdgsvv hgartllasa aviggamkll
      181 rhasdaclsq isfagtaeat lkaqgelsve klyigitqte gsggaglvtf fkdrssltat
      241 tpysdvrvds sndllarlap vipkdfalki gnssvitgge dgvvvglegv mkglqvstkf
      301 qpsdpqlnlt lnldglsvrs kfpvltlrsl iinakveeni lqiatqvnsl sviydhkdwe
      361 tvlcssdnkv stpvasmtlk kklpwldirv dtelydsslc vklldvheas cgvahmkfsi
      421 ntltetedsw ntelvvesly ctlngsntvt letshqwgtp lsvgvllatt rtnaigiavd
      481 tlvtewslel arflqeaykc cqrynskrae tmkqarddsd nvaqlnlkit ncnlffiaen
      541 nlsimvrldt anvehntkrl vavtegvsai tlnknepiic qhaqalahpf lairkckgav
      601 tseminisld gtsfawspnl hlrllqlwle sadfrsiide daskigiqge rmekqrrvid
      661 vlatsgisls idispkhyle lssdqlrwsq gewklnyvrv aldmadiimi egfelvrvfd
      721 nrevrlerqs negfvldwnn qtwmlniesi kacfpyehqf tdaiqnelfs ikkwlkeihs
      781 gssgkkerrp lpcdlvikik ewvfevsddp fevrlrdnye lledeykesl krqamldakv
      841 qelcrahlll pqgkveelyd slnkknaeiy vqrwkqmqra gpartrlfaw smldieilal
      901 adpsidgten atkalremda etpwpedgle fntlwvrgis lkcaewklql rdfpqplllv
      961 eslsvwgrla gaealappra rrtvrieiga pwedivverg mtslkyyhdl nwdidkfrya
     1021 fgpcwepvia qcnlsfekii hpsrdpspsl pfwdkmrlal hgrltlcvkq ltvllhgsld
     1081 pyntteemel twteleldwt qgrivvkgdl dvyvrtasky ddcrllhlpn vrfsiklawv
     1141 clgdprdhha aipcapdrlp eyssnqehds frafrsqnln vslsletkpt espalasapt
     1201 allygstlrw fenlkfilsg atrptrkgpl fknirprrkq lsrhyrkvrl tlafhrfhvs
     1261 ywmsfamqrg fevtggrvac ssehnlslhp idedglihrp raewsvvymn celsdaeiwl
     1321 ksalhedkes aslrqpvekc yclsvarvsy greaamvagv sggdtpthrl vvhdlkgawt
     1381 ktnrdvafal fdsfiktqql kknlstaalk gfhreretts ssssssstsh knhrtrsvev
     1441 qnattqinss satkpqqgea vgmlqrliae aankpvafsd dlsvqtrgqq lrglaachqd
     1501 dvlhknwlia lvnsqvllkg ietrgyvils aakaeilqri hqpvwkertl vskttwvgsl
     1561 ecmqyyatvs anideniddn imwltldniq ekdstqviag lpdvpalvgs gqsvggvvsq
     1621 tvgggggpqh qlqrivsrck ceffyvgygq aldvgsidqv ppppreevsp werkdlsaad
     1681 aftlmhhdld vctnslqyam ildivnnlll yveprrkeas erlqrmrfql qlhsvedqkr
     1741 piqqlqntvr glvaklrrle retylvqkal aeesspellt emerlearvf eckeqlgaka
     1801 eeldvmlscy kettapatas ttlkdkpaav arvaeicfkh aqwrltdadg qlgiadlilt
     1861 nflytktskt ddsvehllel gyvrmtnllp nqiytevltp telqsnmpvd rqralrvfcr
     1921 ekapvagisv kehfeinvvp ltigltkkff ntmlkfcfpe rdpegieepp apqrdssfyv
     1981 pierrddvek mkeradknkl fiyikipevp vrvsykgnke knledvrdfa lviptleyhn
     2041 vtwtwldlll amksdsrrvi lsqaikqklq ikprgpqees apqeedkarl llgarhlpgd
     2101 arkkgvfkfk
//
LOCUS       XP_034194734             455 aa            linear   INV 03-SEP-2025
DEFINITION  inactive ubiquitin carboxyl-terminal hydrolase MINDY-4B isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034194734
VERSION     XP_034194734.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338843.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194734.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..455
                     /product="inactive ubiquitin carboxyl-terminal hydrolase
                     MINDY-4B isoform X1"
                     /calculated_mol_wt=50292
     Region          83..451
                     /region_name="MINDY-3_4_CD"
                     /note="Deubiquitinating enzyme MINDY-3/4, conserved
                     domain; pfam13898"
                     /db_xref="CDD:464029"
     CDS             1..455
                     /gene="LOC117610954"
                     /coded_by="XM_034338843.2:327..1694"
                     /db_xref="GeneID:117610954"
ORIGIN      
        1 miedtpagme eleetkesnn scstttssas vrgkrhctem raekpvylrq kekiiyprnt
       61 rtltktvvig gspideetcr alrtlvfgsc aspprpewar tgltlrpygq nlafglrapr
      121 nttrglvtav qaimlkqflf kcnkqdaras pesllkpsyd rqidvltsai aeiiwrcagg
      181 fvaqntgsqn qgvtgnasta raiialpqei pyvqhtvryf qdgltetlhl feldsledle
      241 ifikrylylf qdeggpgavl llysallsrg lpkvradled pkasilsgcs eegptaivil
      301 sltgrasphl hngvlhvgde dtyaipqwgv lvrsevgflv hegdgqinkq pgsrlktpsl
      361 piwvtlchgh hgvlfntnre llrnyhaerr fevqyftcgg shatltvdtr snsendlefe
      421 gpskectdia atpleklirs kwqdaciqwn gaplm
//
LOCUS       XP_034194799             426 aa            linear   INV 03-SEP-2025
DEFINITION  putative ubiquitin carboxyl-terminal hydrolase MINDY-4 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034194799
VERSION     XP_034194799.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338908.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="putative ubiquitin carboxyl-terminal hydrolase
                     MINDY-4 isoform X2"
                     /calculated_mol_wt=46923
     Region          54..422
                     /region_name="MINDY-3_4_CD"
                     /note="Deubiquitinating enzyme MINDY-3/4, conserved
                     domain; pfam13898"
                     /db_xref="CDD:464029"
     CDS             1..426
                     /gene="LOC117610954"
                     /coded_by="XM_034338908.2:137..1417"
                     /db_xref="GeneID:117610954"
ORIGIN      
        1 mllsaraarc aalmgepdel ypekiiyprn trtltktvvi ggspideetc ralrtlvfgs
       61 caspprpewa rtgltlrpyg qnlafglrap rnttrglvta vqaimlkqfl fkcnkqdara
      121 spesllkpsy drqidvltsa iaeiiwrcag gfvaqntgsq nqgvtgnast araiialpqe
      181 ipyvqhtvry fqdgltetlh lfeldsledl eifikrylyl fqdeggpgav lllysallsr
      241 glpkvradle dpkasilsgc seegptaivi lsltgrasph lhngvlhvgd edtyaipqwg
      301 vlvrsevgfl vhegdgqink qpgsrlktps lpiwvtlchg hhgvlfntnr ellrnyhaer
      361 rfevqyftcg gshatltvdt rsnsendlef egpskectdi aatpleklir skwqdaciqw
      421 ngaplm
//
LOCUS       XP_034173946             776 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601377 [Osmia lignaria lignaria].
ACCESSION   XP_034173946
VERSION     XP_034173946.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318055.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173946.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..776
                     /product="uncharacterized protein LOC117601377"
                     /calculated_mol_wt=90014
     Region          722..768
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     CDS             1..776
                     /gene="LOC117601377"
                     /coded_by="XM_034318055.2:237..2567"
                     /db_xref="GeneID:117601377"
ORIGIN      
        1 mlklliyspi wifitlllia fttrtkehni intinssscy nynyqqdcrw lnkfdntfvn
       61 yihafqglit iflnetcmyd yteinnlknt vkdsdklqeh qknkvhdist ytvkkqipnf
      121 linpiycypn dhlkpklafv vnnisqnlvk gnekernlin ykwkrrtlca ihlddkhstq
      181 tsfrdndank eagektlidy eeplliddte dpeslethvr vkrsksdsgp fsslryqqey
      241 lrdlansfpr ntidessydd ddisfdakre iksqefhvne detdrlkvtd tnqetasakd
      301 dssfrnqrai denmeiqnlp ddllavdlvr kkrnnligkt ipdsknlnde tekhlniedk
      361 nkemsslvaa nlkahllrlk rraknnrhek nsnaknkkkk hagkkrnkns prsignvhrk
      421 sanklrtpre rksngarseg ksnvgvakvv skknnknnfh rrfvkskesk ndrssknlal
      481 ennpdnselv ressvgtesn tddvtvgsdd nadaqdqnkq qtypettklr ekrnkntegs
      541 hgflnkedel ryyenirepe pemhrndgkk rrsmtnevks dravrsieev kdlakklvtk
      601 vnelqnfina eesktngrre rrvetraidd lcsnvstayd afketpkiiq kcvstnrkps
      661 pspakiiver kivpmkmnke gkfvnegkeh stmekkrsvi rrvpkttsrm irrenekahh
      721 kwgrwtdwss csvtcgkgrq irwryclrdc staetemeek acqlpacppg kflgif
//
LOCUS       XP_034174316             987 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601554 [Osmia lignaria lignaria].
ACCESSION   XP_034174316
VERSION     XP_034174316.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318425.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174316.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..987
                     /product="uncharacterized protein LOC117601554"
                     /calculated_mol_wt=109435
     Region          300..>477
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Region          <798..964
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..987
                     /gene="LOC117601554"
                     /coded_by="XM_034318425.2:2176..5139"
                     /db_xref="GeneID:117601554"
ORIGIN      
        1 mtdrnenflc etedessgqp ipykpacnwn nnndihgngs rnivkkdggl cvikdsnyfq
       61 rpvnhykdqt nlngrhpkra pivgwqngsp tvfpstpcgt pdpdtletid lsrsqsvvst
      121 kssgicqdsg ntedlsslad erllqstpar tidrsvesls pdkdtfltls pdigrskdls
      181 laddledkid llspgtdcte drvpptpmet sylsspnltl gpsptnkhel aalnfeshpi
      241 hrteeisnfq sypdtrtiit pnpgfreddc rdsidsdldl dgdlaknqtv kpkvpdggwg
      301 wvvvlaslii smiadgvsfs fgllyieflq efgaskskta wigslfmavp llsgpimsal
      361 vdrygcramt ilgglisgig fvlscfsnti evmyltfgvi agiglglcyv tavvsiaywf
      421 dkkrtlavgl gacgtgigtf vyapmttffi eeygwrgtcl mlagtffnmi vcgtvmrdpe
      481 wwiieqqkqn gttpkksttk secdrsiasp edefpgveel rrmlksghtp eyllqilstt
      541 tedplngdik frsvvnlptf vkhnekvpve vlellasnpr lykvilenyp nllscrsysd
      601 kklhesvgev gnksvvtmsm rlkqandgpk gedshatnny hsgsacnsik nhitkkivkk
      661 eseeanpllt kelehttteg rkvksasrgi gelncgvvrt dslpwlrrqf ntnthyfkdi
      721 rihrnsvmyr gaalnlhkyr lrasscpdiy rnsmttlake neekwyselv dllkgmmdfs
      781 mflelhflll slstillftw fivpyfylae hltrnsytes daanllsiig itntigmigl
      841 gwagdqpwmn vsktytwclv acgiatvlms iltpyynllm vtsaafglff asnfsftpvi
      901 lveliplerf ttayglsllc qgignllgpp lagwlfdvtd swdlsfhmag gwiivsgllv
      961 glvpytknrk iwgegpieme rekdcla
//
LOCUS       XP_034174325             987 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601554 [Osmia lignaria lignaria].
ACCESSION   XP_034174325
VERSION     XP_034174325.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174325.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..987
                     /product="uncharacterized protein LOC117601554"
                     /calculated_mol_wt=109435
     Region          300..>477
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Region          <798..964
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..987
                     /gene="LOC117601554"
                     /coded_by="XM_034318434.2:2245..5208"
                     /db_xref="GeneID:117601554"
ORIGIN      
        1 mtdrnenflc etedessgqp ipykpacnwn nnndihgngs rnivkkdggl cvikdsnyfq
       61 rpvnhykdqt nlngrhpkra pivgwqngsp tvfpstpcgt pdpdtletid lsrsqsvvst
      121 kssgicqdsg ntedlsslad erllqstpar tidrsvesls pdkdtfltls pdigrskdls
      181 laddledkid llspgtdcte drvpptpmet sylsspnltl gpsptnkhel aalnfeshpi
      241 hrteeisnfq sypdtrtiit pnpgfreddc rdsidsdldl dgdlaknqtv kpkvpdggwg
      301 wvvvlaslii smiadgvsfs fgllyieflq efgaskskta wigslfmavp llsgpimsal
      361 vdrygcramt ilgglisgig fvlscfsnti evmyltfgvi agiglglcyv tavvsiaywf
      421 dkkrtlavgl gacgtgigtf vyapmttffi eeygwrgtcl mlagtffnmi vcgtvmrdpe
      481 wwiieqqkqn gttpkksttk secdrsiasp edefpgveel rrmlksghtp eyllqilstt
      541 tedplngdik frsvvnlptf vkhnekvpve vlellasnpr lykvilenyp nllscrsysd
      601 kklhesvgev gnksvvtmsm rlkqandgpk gedshatnny hsgsacnsik nhitkkivkk
      661 eseeanpllt kelehttteg rkvksasrgi gelncgvvrt dslpwlrrqf ntnthyfkdi
      721 rihrnsvmyr gaalnlhkyr lrasscpdiy rnsmttlake neekwyselv dllkgmmdfs
      781 mflelhflll slstillftw fivpyfylae hltrnsytes daanllsiig itntigmigl
      841 gwagdqpwmn vsktytwclv acgiatvlms iltpyynllm vtsaafglff asnfsftpvi
      901 lveliplerf ttayglsllc qgignllgpp lagwlfdvtd swdlsfhmag gwiivsgllv
      961 glvpytknrk iwgegpieme rekdcla
//
LOCUS       XP_034174336             987 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601554 [Osmia lignaria lignaria].
ACCESSION   XP_034174336
VERSION     XP_034174336.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318445.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174336.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..987
                     /product="uncharacterized protein LOC117601554"
                     /calculated_mol_wt=109435
     Region          300..>477
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Region          <798..964
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..987
                     /gene="LOC117601554"
                     /coded_by="XM_034318445.2:2173..5136"
                     /db_xref="GeneID:117601554"
ORIGIN      
        1 mtdrnenflc etedessgqp ipykpacnwn nnndihgngs rnivkkdggl cvikdsnyfq
       61 rpvnhykdqt nlngrhpkra pivgwqngsp tvfpstpcgt pdpdtletid lsrsqsvvst
      121 kssgicqdsg ntedlsslad erllqstpar tidrsvesls pdkdtfltls pdigrskdls
      181 laddledkid llspgtdcte drvpptpmet sylsspnltl gpsptnkhel aalnfeshpi
      241 hrteeisnfq sypdtrtiit pnpgfreddc rdsidsdldl dgdlaknqtv kpkvpdggwg
      301 wvvvlaslii smiadgvsfs fgllyieflq efgaskskta wigslfmavp llsgpimsal
      361 vdrygcramt ilgglisgig fvlscfsnti evmyltfgvi agiglglcyv tavvsiaywf
      421 dkkrtlavgl gacgtgigtf vyapmttffi eeygwrgtcl mlagtffnmi vcgtvmrdpe
      481 wwiieqqkqn gttpkksttk secdrsiasp edefpgveel rrmlksghtp eyllqilstt
      541 tedplngdik frsvvnlptf vkhnekvpve vlellasnpr lykvilenyp nllscrsysd
      601 kklhesvgev gnksvvtmsm rlkqandgpk gedshatnny hsgsacnsik nhitkkivkk
      661 eseeanpllt kelehttteg rkvksasrgi gelncgvvrt dslpwlrrqf ntnthyfkdi
      721 rihrnsvmyr gaalnlhkyr lrasscpdiy rnsmttlake neekwyselv dllkgmmdfs
      781 mflelhflll slstillftw fivpyfylae hltrnsytes daanllsiig itntigmigl
      841 gwagdqpwmn vsktytwclv acgiatvlms iltpyynllm vtsaafglff asnfsftpvi
      901 lveliplerf ttayglsllc qgignllgpp lagwlfdvtd swdlsfhmag gwiivsgllv
      961 glvpytknrk iwgegpieme rekdcla
//
LOCUS       XP_076546226             920 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 9 [Osmia lignaria lignaria].
ACCESSION   XP_076546226
VERSION     XP_076546226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690111.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..920
                     /product="monocarboxylate transporter 9"
                     /calculated_mol_wt=102778
     Region          273..893
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(284..285,288..289,292,327,378..379,381..383,386,405,
                     408..409,412,558,561..562,570..572,578,704,715,818..819,
                     823,827,843,846..847,850..851,854)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..920
                     /gene="LOC117601563"
                     /coded_by="XM_076690111.1:1089..3851"
                     /db_xref="GeneID:117601563"
ORIGIN      
        1 maewakfnge erpkeiinte ireeenngvs rndlrngeav srrieeelll errrsscnrt
       61 pqetprkvdc rksseivsae lnslvkdssr lrrnsdrigi lseeqskaln dqsyendvat
      121 ekssededdn clerafdgye esgsrirrea kqelqnsvkr prslykedvp spqkrksvfa
      181 egrnsifgsp trilskkhsp seksteklvn gklsreekqq hhvqfnkdsk kqqsredraq
      241 pspsiptstt gssddnssld qlceatppdg gwgwvvvaas fmvnliadgi tfsfgviyve
      301 flnyfgegks ktawigslfm ampllsgpva sfltdrygcr rvsiagsila ttgfvissya
      361 nsmevlvftf gvlagfglsl cfvaavviva yyfdkkrsfa tglsvcgsgi gtfifapvtq
      421 yllaeygwrg ttlilaglfl nlavcgclmr dlewtttrak akteerrknr ekkrsriqss
      481 svdsfyasns lntltimenl rlheeedgek lfsslvslpt fvkngekvpl evlellstrk
      541 nvynvllqny psllissrsf sdsgalhdql stpsarfvpt psplselkgd enkdkvlpsn
      601 deqtpqqqad aawrswwlkk inldsslrrs stlehtrrlp taylkdirvh rhsltyrgam
      661 lninryrlra sscpdiyrns mttiaktklv wyaglwefwd livdmldfsh fadsrfllfa
      721 isnfllhtwy dvpyvyltdn aiemgfsetd asilisvigi tnmggeiilg wagdrawvna
      781 sivyavcmal cgavtalipv vigsyyslca isgafglfig anysltsiil velitlerft
      841 naygllllvq gvanlmgppl agwlyditgt ydlsfylagf fialsgvlll amplislyrk
      901 clngskkeet dkdfssmnsv
//
LOCUS       XP_076546227             920 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 9 [Osmia lignaria lignaria].
ACCESSION   XP_076546227
VERSION     XP_076546227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690112.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..920
                     /product="monocarboxylate transporter 9"
                     /calculated_mol_wt=102778
     Region          273..893
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(284..285,288..289,292,327,378..379,381..383,386,405,
                     408..409,412,558,561..562,570..572,578,704,715,818..819,
                     823,827,843,846..847,850..851,854)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..920
                     /gene="LOC117601563"
                     /coded_by="XM_076690112.1:2060..4822"
                     /db_xref="GeneID:117601563"
ORIGIN      
        1 maewakfnge erpkeiinte ireeenngvs rndlrngeav srrieeelll errrsscnrt
       61 pqetprkvdc rksseivsae lnslvkdssr lrrnsdrigi lseeqskaln dqsyendvat
      121 ekssededdn clerafdgye esgsrirrea kqelqnsvkr prslykedvp spqkrksvfa
      181 egrnsifgsp trilskkhsp seksteklvn gklsreekqq hhvqfnkdsk kqqsredraq
      241 pspsiptstt gssddnssld qlceatppdg gwgwvvvaas fmvnliadgi tfsfgviyve
      301 flnyfgegks ktawigslfm ampllsgpva sfltdrygcr rvsiagsila ttgfvissya
      361 nsmevlvftf gvlagfglsl cfvaavviva yyfdkkrsfa tglsvcgsgi gtfifapvtq
      421 yllaeygwrg ttlilaglfl nlavcgclmr dlewtttrak akteerrknr ekkrsriqss
      481 svdsfyasns lntltimenl rlheeedgek lfsslvslpt fvkngekvpl evlellstrk
      541 nvynvllqny psllissrsf sdsgalhdql stpsarfvpt psplselkgd enkdkvlpsn
      601 deqtpqqqad aawrswwlkk inldsslrrs stlehtrrlp taylkdirvh rhsltyrgam
      661 lninryrlra sscpdiyrns mttiaktklv wyaglwefwd livdmldfsh fadsrfllfa
      721 isnfllhtwy dvpyvyltdn aiemgfsetd asilisvigi tnmggeiilg wagdrawvna
      781 sivyavcmal cgavtalipv vigsyyslca isgafglfig anysltsiil velitlerft
      841 naygllllvq gvanlmgppl agwlyditgt ydlsfylagf fialsgvlll amplislyrk
      901 clngskkeet dkdfssmnsv
//
LOCUS       XP_076546228             920 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 9 [Osmia lignaria lignaria].
ACCESSION   XP_076546228
VERSION     XP_076546228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690113.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..920
                     /product="monocarboxylate transporter 9"
                     /calculated_mol_wt=102778
     Region          273..893
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(284..285,288..289,292,327,378..379,381..383,386,405,
                     408..409,412,558,561..562,570..572,578,704,715,818..819,
                     823,827,843,846..847,850..851,854)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..920
                     /gene="LOC117601563"
                     /coded_by="XM_076690113.1:1580..4342"
                     /db_xref="GeneID:117601563"
ORIGIN      
        1 maewakfnge erpkeiinte ireeenngvs rndlrngeav srrieeelll errrsscnrt
       61 pqetprkvdc rksseivsae lnslvkdssr lrrnsdrigi lseeqskaln dqsyendvat
      121 ekssededdn clerafdgye esgsrirrea kqelqnsvkr prslykedvp spqkrksvfa
      181 egrnsifgsp trilskkhsp seksteklvn gklsreekqq hhvqfnkdsk kqqsredraq
      241 pspsiptstt gssddnssld qlceatppdg gwgwvvvaas fmvnliadgi tfsfgviyve
      301 flnyfgegks ktawigslfm ampllsgpva sfltdrygcr rvsiagsila ttgfvissya
      361 nsmevlvftf gvlagfglsl cfvaavviva yyfdkkrsfa tglsvcgsgi gtfifapvtq
      421 yllaeygwrg ttlilaglfl nlavcgclmr dlewtttrak akteerrknr ekkrsriqss
      481 svdsfyasns lntltimenl rlheeedgek lfsslvslpt fvkngekvpl evlellstrk
      541 nvynvllqny psllissrsf sdsgalhdql stpsarfvpt psplselkgd enkdkvlpsn
      601 deqtpqqqad aawrswwlkk inldsslrrs stlehtrrlp taylkdirvh rhsltyrgam
      661 lninryrlra sscpdiyrns mttiaktklv wyaglwefwd livdmldfsh fadsrfllfa
      721 isnfllhtwy dvpyvyltdn aiemgfsetd asilisvigi tnmggeiilg wagdrawvna
      781 sivyavcmal cgavtalipv vigsyyslca isgafglfig anysltsiil velitlerft
      841 naygllllvq gvanlmgppl agwlyditgt ydlsfylagf fialsgvlll amplislyrk
      901 clngskkeet dkdfssmnsv
//
LOCUS       XP_076546229             920 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 9 [Osmia lignaria lignaria].
ACCESSION   XP_076546229
VERSION     XP_076546229.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690114.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..920
                     /product="monocarboxylate transporter 9"
                     /calculated_mol_wt=102778
     Region          273..893
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(284..285,288..289,292,327,378..379,381..383,386,405,
                     408..409,412,558,561..562,570..572,578,704,715,818..819,
                     823,827,843,846..847,850..851,854)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..920
                     /gene="LOC117601563"
                     /coded_by="XM_076690114.1:702..3464"
                     /db_xref="GeneID:117601563"
ORIGIN      
        1 maewakfnge erpkeiinte ireeenngvs rndlrngeav srrieeelll errrsscnrt
       61 pqetprkvdc rksseivsae lnslvkdssr lrrnsdrigi lseeqskaln dqsyendvat
      121 ekssededdn clerafdgye esgsrirrea kqelqnsvkr prslykedvp spqkrksvfa
      181 egrnsifgsp trilskkhsp seksteklvn gklsreekqq hhvqfnkdsk kqqsredraq
      241 pspsiptstt gssddnssld qlceatppdg gwgwvvvaas fmvnliadgi tfsfgviyve
      301 flnyfgegks ktawigslfm ampllsgpva sfltdrygcr rvsiagsila ttgfvissya
      361 nsmevlvftf gvlagfglsl cfvaavviva yyfdkkrsfa tglsvcgsgi gtfifapvtq
      421 yllaeygwrg ttlilaglfl nlavcgclmr dlewtttrak akteerrknr ekkrsriqss
      481 svdsfyasns lntltimenl rlheeedgek lfsslvslpt fvkngekvpl evlellstrk
      541 nvynvllqny psllissrsf sdsgalhdql stpsarfvpt psplselkgd enkdkvlpsn
      601 deqtpqqqad aawrswwlkk inldsslrrs stlehtrrlp taylkdirvh rhsltyrgam
      661 lninryrlra sscpdiyrns mttiaktklv wyaglwefwd livdmldfsh fadsrfllfa
      721 isnfllhtwy dvpyvyltdn aiemgfsetd asilisvigi tnmggeiilg wagdrawvna
      781 sivyavcmal cgavtalipv vigsyyslca isgafglfig anysltsiil velitlerft
      841 naygllllvq gvanlmgppl agwlyditgt ydlsfylagf fialsgvlll amplislyrk
      901 clngskkeet dkdfssmnsv
//
LOCUS       XP_034174351             920 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 9 [Osmia lignaria lignaria].
ACCESSION   XP_034174351
VERSION     XP_034174351.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318460.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174351.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..920
                     /product="monocarboxylate transporter 9"
                     /calculated_mol_wt=102778
     Region          273..893
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(284..285,288..289,292,327,378..379,381..383,386,405,
                     408..409,412,558,561..562,570..572,578,704,715,818..819,
                     823,827,843,846..847,850..851,854)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..920
                     /gene="LOC117601563"
                     /coded_by="XM_034318460.2:1772..4534"
                     /db_xref="GeneID:117601563"
ORIGIN      
        1 maewakfnge erpkeiinte ireeenngvs rndlrngeav srrieeelll errrsscnrt
       61 pqetprkvdc rksseivsae lnslvkdssr lrrnsdrigi lseeqskaln dqsyendvat
      121 ekssededdn clerafdgye esgsrirrea kqelqnsvkr prslykedvp spqkrksvfa
      181 egrnsifgsp trilskkhsp seksteklvn gklsreekqq hhvqfnkdsk kqqsredraq
      241 pspsiptstt gssddnssld qlceatppdg gwgwvvvaas fmvnliadgi tfsfgviyve
      301 flnyfgegks ktawigslfm ampllsgpva sfltdrygcr rvsiagsila ttgfvissya
      361 nsmevlvftf gvlagfglsl cfvaavviva yyfdkkrsfa tglsvcgsgi gtfifapvtq
      421 yllaeygwrg ttlilaglfl nlavcgclmr dlewtttrak akteerrknr ekkrsriqss
      481 svdsfyasns lntltimenl rlheeedgek lfsslvslpt fvkngekvpl evlellstrk
      541 nvynvllqny psllissrsf sdsgalhdql stpsarfvpt psplselkgd enkdkvlpsn
      601 deqtpqqqad aawrswwlkk inldsslrrs stlehtrrlp taylkdirvh rhsltyrgam
      661 lninryrlra sscpdiyrns mttiaktklv wyaglwefwd livdmldfsh fadsrfllfa
      721 isnfllhtwy dvpyvyltdn aiemgfsetd asilisvigi tnmggeiilg wagdrawvna
      781 sivyavcmal cgavtalipv vigsyyslca isgafglfig anysltsiil velitlerft
      841 naygllllvq gvanlmgppl agwlyditgt ydlsfylagf fialsgvlll amplislyrk
      901 clngskkeet dkdfssmnsv
//
LOCUS       XP_034174364             767 aa            linear   INV 03-SEP-2025
DEFINITION  TBC1 domain family member 16 [Osmia lignaria lignaria].
ACCESSION   XP_034174364
VERSION     XP_034174364.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318473.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174364.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..767
                     /product="TBC1 domain family member 16"
                     /calculated_mol_wt=88408
     Region          452..684
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..767
                     /gene="TBC1D16"
                     /coded_by="XM_034318473.2:362..2665"
                     /db_xref="GeneID:117601568"
ORIGIN      
        1 mplpnilrka ssyilgdcnq dnrrtmyqdg ealfcknnvc vhpptlmrqn sdvvhhpgym
       61 aitcrfnkns nvptlhlswi pnstlrkhpt tlenlsakkv dcivhrdadr sshhsqisee
      121 sydlksdyen tsekvdvcle vkepvncgdc ervcsggfpr nardevrdan lkndardnss
      181 edqnvsetke rdanvnrrvd yyqsdnssvt ihedndhsis lnrlrsesld ckhgenqkdq
      241 kiesqdvdrk stdeelrypy cddvsfksrs msltstcsls vgvsseaeei pswmrspell
      301 alqhnltfpe sataspvtlr rahrcrrfsv dlsemrslrl ffadpactsg qlvvasresq
      361 ykilhfhhgg ldrlaatlhq whqllyprld tdteeslpyr hfmvcrpevs rdelhpeegq
      421 vpmitslawk dllnergqve ddlalrkgif fgglepalrk ivwpfllhcy syqstyddre
      481 qidairrqey eeiqkrrlgm speqaeyfwr nvvcivekdv vrtdrgnpyy agednpniev
      541 mknillnyav ynsrlgytqg msdllaplla elnseieafw cfaglmqrsv avctptdvdm
      601 drnlcylrel vrimvpdfya hlqkhtdafe llfchrwill clkrefptev alvmweacwv
      661 nyltdhfhlf lclaimcvya ddviaqdlrt demllhfssl amymdgnlil rkargllhhf
      721 rqlvrlpctl aglcrqcgpg mwdsthdpvi ecvghddapc pylnnye
//
LOCUS       XP_076546230             669 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601621 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546230
VERSION     XP_076546230.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690115.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..669
                     /product="uncharacterized protein LOC117601621 isoform X1"
                     /calculated_mol_wt=76456
     Region          501..626
                     /region_name="WS_DGAT_C"
                     /note="WS/DGAT C-terminal domain; pfam06974"
                     /db_xref="CDD:462057"
     CDS             1..669
                     /gene="LOC117601621"
                     /coded_by="XM_076690115.1:197..2206"
                     /db_xref="GeneID:117601621"
ORIGIN      
        1 mtafnimdvl gisllihltf slmllvlsvp lllfvypvva wsrkvwisfv kwkypnvvvv
       61 eensirtild qfrnhgiytl liqarsiaen irghlihlts srrflragls trwglyvwkd
      121 ldyfsidnhl lnspcsfrgr pitesniqdy vsdltskffp tgqppwqvhv incflrgeey
      181 qvclvrvhhl llrqehlala dflplrystd gwscqetdsp ftnlyaepsa lpklyqklte
      241 sfsnywnefl ynndpnerpe ilkkqigifh cakigvivlv sslkeltrqy rkrerpgffa
      301 ffsiihreas krsfgplvfl ralvrslnpf eliydailwf wylgvtltlk tpilllrelr
      361 alksrhkhyy pdtltsvlwy yvplivlatm evlsitwiai rapktileel flkhpqtnrl
      421 qttspcgrkv vawseevefe ilrkissmtg ateaeillav mvdslkeyfr hsgvripddv
      481 latgkfisqr sifvqnhear gilclalptr tplfeddlve ilqviqknvr earskqsamy
      541 aitaaeasfg lissclpsll lkvmlnqltr ryslslthvd gdlpvegvdt vmywrppqgn
      601 cnmsitlhry gngvrlgvmg daligpehsi itrtfpksie nlanvvgvpr tpsrhstpsp
      661 aspstspgh
//
LOCUS       XP_076546231             668 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601621 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546231
VERSION     XP_076546231.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690116.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="uncharacterized protein LOC117601621 isoform X2"
                     /calculated_mol_wt=76357
     Region          500..625
                     /region_name="WS_DGAT_C"
                     /note="WS/DGAT C-terminal domain; pfam06974"
                     /db_xref="CDD:462057"
     CDS             1..668
                     /gene="LOC117601621"
                     /coded_by="XM_076690116.1:198..2204"
                     /db_xref="GeneID:117601621"
ORIGIN      
        1 mtafnimdvl gisllihltf slmllvlsvp lllfvypvaw srkvwisfvk wkypnvvvve
       61 ensirtildq frnhgiytll iqarsiaeni rghlihltss rrflraglst rwglyvwkdl
      121 dyfsidnhll nspcsfrgrp itesniqdyv sdltskffpt gqppwqvhvi ncflrgeeyq
      181 vclvrvhhll lrqehlalad flplrystdg wscqetdspf tnlyaepsal pklyqkltes
      241 fsnywnefly nndpnerpei lkkqigifhc akigvivlvs slkeltrqyr krerpgffaf
      301 fsiihreask rsfgplvflr alvrslnpfe liydailwfw ylgvtltlkt pilllrelra
      361 lksrhkhyyp dtltsvlwyy vplivlatme vlsitwiair apktileelf lkhpqtnrlq
      421 ttspcgrkvv awseevefei lrkissmtga teaeillavm vdslkeyfrh sgvripddvl
      481 atgkfisqrs ifvqnhearg ilclalptrt plfeddlvei lqviqknvre arskqsamya
      541 itaaeasfgl issclpslll kvmlnqltrr yslslthvdg dlpvegvdtv mywrppqgnc
      601 nmsitlhryg ngvrlgvmgd aligpehsii trtfpksien lanvvgvprt psrhstpspa
      661 spstspgh
//
LOCUS       XP_076546232             631 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601621 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546232
VERSION     XP_076546232.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690117.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="uncharacterized protein LOC117601621 isoform X3"
                     /calculated_mol_wt=72258
     Region          463..588
                     /region_name="WS_DGAT_C"
                     /note="WS/DGAT C-terminal domain; pfam06974"
                     /db_xref="CDD:462057"
     CDS             1..631
                     /gene="LOC117601621"
                     /coded_by="XM_076690117.1:19..1914"
                     /db_xref="GeneID:117601621"
ORIGIN      
        1 mawsrkvwis fvkwkypnvv vveensirti ldqfrnhgiy tlliqarsia enirghlihl
       61 tssrrflrag lstrwglyvw kdldyfsidn hllnspcsfr grpitesniq dyvsdltskf
      121 fptgqppwqv hvincflrge eyqvclvrvh hlllrqehla ladflplrys tdgwscqetd
      181 spftnlyaep salpklyqkl tesfsnywne flynndpner peilkkqigi fhcakigviv
      241 lvsslkeltr qyrkrerpgf faffsiihre askrsfgplv flralvrsln pfeliydail
      301 wfwylgvtlt lktpilllre lralksrhkh yypdtltsvl wyyvplivla tmevlsitwi
      361 airapktile elflkhpqtn rlqttspcgr kvvawseeve feilrkissm tgateaeill
      421 avmvdslkey frhsgvripd dvlatgkfis qrsifvqnhe argilclalp trtplfeddl
      481 veilqviqkn vrearskqsa myaitaaeas fglissclps lllkvmlnql trryslslth
      541 vdgdlpvegv dtvmywrppq gncnmsitlh rygngvrlgv mgdaligpeh siitrtfpks
      601 ienlanvvgv prtpsrhstp spaspstspg h
//
LOCUS       XP_034174377             597 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent DNA helicase Q1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034174377
VERSION     XP_034174377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..597
                     /product="ATP-dependent DNA helicase Q1 isoform X1"
                     /calculated_mol_wt=68088
     Region          85..533
                     /region_name="recQ_fam"
                     /note="ATP-dependent DNA helicase, RecQ family; TIGR00614"
                     /db_xref="CDD:129701"
     CDS             1..597
                     /gene="LOC117601577"
                     /coded_by="XM_034318486.2:142..1935"
                     /db_xref="GeneID:117601577"
ORIGIN      
        1 msasnskdvi ilddenqate eseiaaidye lkqidseiqk ledrkkvlvq rkeklrddal
       61 lkkslyvskk dwgkedfsws kdlrnalkdv fkikklrdlq lptmnaimsn edvilimptg
      121 ggkslcyqlp aviskgitiv vsplvalmed qlhglhknnv kammlsakgn kedvkiimna
      181 lvdkksdlkl iyvtpeymak snrfmsklqk afemkrldrf aidevhccsq wghdfrpdyk
      241 flgilksmfp gvpilgltat apakiivdvq kmldiqgclv lratfnrpnl fyevrrkpad
      301 kstclamien llktrfkdks giiytttikd aeqlttdlrs ldlkvgcyha mlepqyrsev
      361 yskwisgnyq avvatiafgl gidkpdvrfv ihhcisksme nfyqesgrag rdgrksaciv
      421 lyrlpdvfkl stmvfqdkvg lqnlykmlay cldltscrrs liathfeetw tesdcaemcd
      481 hcrkrkekke vniapycrql yeimtkavqs etrltalklt dawygkgatt lrvhsipvpk
      541 ftresgeaiv ghllvngylq edfhfsayst isylkrgpka vlvldkdhki pfnyelh
//
LOCUS       XP_034174388             561 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent DNA helicase Q1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034174388
VERSION     XP_034174388.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318497.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="ATP-dependent DNA helicase Q1 isoform X2"
                     /calculated_mol_wt=63874
     Region          85..484
                     /region_name="recQ_fam"
                     /note="ATP-dependent DNA helicase, RecQ family; TIGR00614"
                     /db_xref="CDD:129701"
     CDS             1..561
                     /gene="LOC117601577"
                     /coded_by="XM_034318497.2:142..1827"
                     /db_xref="GeneID:117601577"
ORIGIN      
        1 msasnskdvi ilddenqate eseiaaidye lkqidseiqk ledrkkvlvq rkeklrddal
       61 lkkslyvskk dwgkedfsws kdlrnalkdv fkikklrdlq lptmnaimsn edvilimptg
      121 ggkslcyqlp aviskgitiv vsplvalmed qlhglhknnv kammlsakgn kedvkiimna
      181 lvdkksdlkl iyvtpeymak snrfmsklqk afemkrldrf aidevhccsq wghdfrpdyk
      241 flgilksmfp gvpilgltat apakiivdvq kmldiqgclv lratfnrpnl fyevrrkpad
      301 kstclamien llktrfkdks giiytttikd aeqlttdlrs ldlkvgcyha mlepqyrsev
      361 yskwisgnyq avvatiafgl gidkpdvrfv ihhcisksme nfyqesgrag rdgrksaciv
      421 lyrlpdvfkl stmvfqdkvg lqnlykmlay cldltscrrs liathfeetw tesdcaemcd
      481 hcrkhawygk gattlrvhsi pvpkftresg eaivghllvn gylqedfhfs aystisylkr
      541 gpkavlvldk dhkipfnyel h
//
LOCUS       XP_034174399             494 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent DNA helicase Q1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034174399
VERSION     XP_034174399.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318508.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="ATP-dependent DNA helicase Q1 isoform X3"
                     /calculated_mol_wt=56037
     Region          1..430
                     /region_name="recQ_fam"
                     /note="ATP-dependent DNA helicase, RecQ family; TIGR00614"
                     /db_xref="CDD:129701"
     CDS             1..494
                     /gene="LOC117601577"
                     /coded_by="XM_034318508.2:406..1890"
                     /db_xref="GeneID:117601577"
ORIGIN      
        1 mnaimsnedv ilimptgggk slcyqlpavi skgitivvsp lvalmedqlh glhknnvkam
       61 mlsakgnked vkiimnalvd kksdlkliyv tpeymaksnr fmsklqkafe mkrldrfaid
      121 evhccsqwgh dfrpdykflg ilksmfpgvp ilgltatapa kiivdvqkml diqgclvlra
      181 tfnrpnlfye vrrkpadkst clamienllk trfkdksgii ytttikdaeq lttdlrsldl
      241 kvgcyhamle pqyrsevysk wisgnyqavv atiafglgid kpdvrfvihh cisksmenfy
      301 qesgragrdg rksacivlyr lpdvfklstm vfqdkvglqn lykmlaycld ltscrrslia
      361 thfeetwtes dcaemcdhcr krkekkevni apycrqlyei mtkavqsetr ltalkltdaw
      421 ygkgattlrv hsipvpkftr esgeaivghl lvngylqedf hfsaystisy lkrgpkavlv
      481 ldkdhkipfn yelh
//
LOCUS       XP_034174415             559 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 104 homolog isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174415
VERSION     XP_034174415.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318524.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..559
                     /product="transmembrane protein 104 homolog isoform X1"
                     /calculated_mol_wt=63049
     Region          11..>63
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Region          305..559
                     /region_name="PLN02469"
                     /note="hydroxyacylglutathione hydrolase"
                     /db_xref="CDD:178088"
     CDS             1..559
                     /gene="LOC117601580"
                     /coded_by="XM_034318524.2:433..2112"
                     /db_xref="GeneID:117601580"
ORIGIN      
        1 mpeqnsndqy sswvgliyif nlivgtgalt lpavfskagw alglsvilil afisfitvtf
       61 vievmasana ivtwrhiqhr krvcftqdss fnesesemlq tlgesgpsns dsedtplvtp
      121 fssspdradm tyryyvirdk iemgqmasif fnkfgitlfy icfavylygd lsiygaavak
      181 tladvactyq plnftcndti pdtelcweey svnrqdayri flsmfvfllg pfvffniqrt
      241 kylqfltsgm rwlaftimiv yavknliihg aqgnpptani marsfstngf yathslstli
      301 kqpnmkvqil palqdnymyl iideasqeaa vvdpvdpdsi lsvvlqnnln ltkvltthhh
      361 wdhaggnakl ckkfnklqvy ggddridalt hkvkhndtfn igrlqvkcla tpchttghic
      421 yyvvgdqnpp alfsgdtlfa ggcgrffegt aeqmytalik ilgslpdetk vycgheytgn
      481 nlkfgkfvep ensaihrkme wvriqreknh ptvpstiqee kltnpfmrvh eksvmdhteq
      541 kdpiqtmafl rrekdnfks
//
LOCUS       XP_034174426             544 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 104 homolog isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174426
VERSION     XP_034174426.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318535.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..544
                     /product="transmembrane protein 104 homolog isoform X2"
                     /calculated_mol_wt=61358
     Region          11..>63
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     Region          290..544
                     /region_name="PLN02469"
                     /note="hydroxyacylglutathione hydrolase"
                     /db_xref="CDD:178088"
     CDS             1..544
                     /gene="LOC117601580"
                     /coded_by="XM_034318535.2:428..2062"
                     /db_xref="GeneID:117601580"
ORIGIN      
        1 mpeqnsndqy sswvgliyif nlivgtgalt lpavfskagw alglsvilil afisfitvtf
       61 vievmasana ivtwrhiqhr krmlqtlges gpsnsdsedt plvtpfsssp dradmtyryy
      121 virdkiemgq masiffnkfg itlfyicfav ylygdlsiyg aavaktladv actyqplnft
      181 cndtipdtel cweeysvnrq dayriflsmf vfllgpfvff niqrtkylqf ltsgmrwlaf
      241 timivyavkn liihgaqgnp ptanimarsf stngfyaths lstlikqpnm kvqilpalqd
      301 nymyliidea sqeaavvdpv dpdsilsvvl qnnlnltkvl tthhhwdhag gnaklckkfn
      361 klqvyggddr idalthkvkh ndtfnigrlq vkclatpcht tghicyyvvg dqnppalfsg
      421 dtlfaggcgr ffegtaeqmy talikilgsl pdetkvycgh eytgnnlkfg kfvepensai
      481 hrkmewvriq reknhptvps tiqeekltnp fmrvheksvm dhteqkdpiq tmaflrrekd
      541 nfks
//
LOCUS       XP_034174436             516 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 104 homolog isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174436
VERSION     XP_034174436.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318545.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174436.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="transmembrane protein 104 homolog isoform X3"
                     /calculated_mol_wt=57734
     Region          11..495
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..516
                     /gene="LOC117601580"
                     /coded_by="XM_034318545.2:488..2038"
                     /db_xref="GeneID:117601580"
ORIGIN      
        1 mpeqnsndqy sswvgliyif nlivgtgalt lpavfskagw alglsvilil afisfitvtf
       61 vievmasana ivtwrhiqhr krvcftqdss fnesesemlq tlgesgpsns dsedtplvtp
      121 fssspdradm tyryyvirdk iemgqmasif fnkfgitlfy icfavylygd lsiygaavak
      181 tladvactyq plnftcndti pdtelcweey svnrqdayri flsmfvfllg pfvffniqrt
      241 kylqfltsgm rwlaftimiv yavknliihg aqgnpptani mgipslfgac iysfmchhsl
      301 palvapiank stlnrfmald yfliaffyll laltgafafe hlddlytldf sprgsgdcsk
      361 snnifivlie yflalfpvft lstsfpiiai tlrnnlqslf lhedtsyscl yklvfpilai
      421 lppymiamst kdlsilvgit gsyagagiqy liptvlvyya rqktnkvigl gvvnkfaspf
      481 fnncwlvfvi awaitcmvlv svnfiqihlq stglkl
//
LOCUS       XP_034174446             501 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 104 homolog isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034174446
VERSION     XP_034174446.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318555.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034174446.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="transmembrane protein 104 homolog isoform X4"
                     /calculated_mol_wt=56043
     Region          11..480
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..501
                     /gene="LOC117601580"
                     /coded_by="XM_034318555.2:486..1991"
                     /db_xref="GeneID:117601580"
ORIGIN      
        1 mpeqnsndqy sswvgliyif nlivgtgalt lpavfskagw alglsvilil afisfitvtf
       61 vievmasana ivtwrhiqhr krmlqtlges gpsnsdsedt plvtpfsssp dradmtyryy
      121 virdkiemgq masiffnkfg itlfyicfav ylygdlsiyg aavaktladv actyqplnft
      181 cndtipdtel cweeysvnrq dayriflsmf vfllgpfvff niqrtkylqf ltsgmrwlaf
      241 timivyavkn liihgaqgnp ptanimgips lfgaciysfm chhslpalva piankstlnr
      301 fmaldyflia ffylllaltg afafehlddl ytldfsprgs gdcsksnnif ivlieyflal
      361 fpvftlstsf piiaitlrnn lqslflhedt sysclyklvf pilailppym iamstkdlsi
      421 lvgitgsyag agiqyliptv lvyyarqktn kviglgvvnk faspffnncw lvfviawait
      481 cmvlvsvnfi qihlqstglk l
//
LOCUS       XP_034174455             301 aa            linear   INV 03-SEP-2025
DEFINITION  hydroxyacylglutathione hydrolase, mitochondrial isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034174455
VERSION     XP_034174455.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318564.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..301
                     /product="hydroxyacylglutathione hydrolase, mitochondrial
                     isoform X5"
                     /calculated_mol_wt=34085
     Region          47..301
                     /region_name="PLN02469"
                     /note="hydroxyacylglutathione hydrolase"
                     /db_xref="CDD:178088"
     CDS             1..301
                     /gene="LOC117601580"
                     /coded_by="XM_034318564.2:288..1193"
                     /db_xref="GeneID:117601580"
ORIGIN      
        1 minamfracp twventftsl yfkarsfstn gfyathslst likqpnmkvq ilpalqdnym
       61 yliideasqe aavvdpvdpd silsvvlqnn lnltkvltth hhwdhaggna klckkfnklq
      121 vyggddrida lthkvkhndt fnigrlqvkc latpchttgh icyyvvgdqn ppalfsgdtl
      181 faggcgrffe gtaeqmytal ikilgslpde tkvycgheyt gnnlkfgkfv epensaihrk
      241 mewvriqrek nhptvpstiq eekltnpfmr vheksvmdht eqkdpiqtma flrrekdnfk
      301 s
//
LOCUS       XP_034174481             376 aa            linear   INV 03-SEP-2025
DEFINITION  actin-5C [Osmia lignaria lignaria].
ACCESSION   XP_034174481
VERSION     XP_034174481.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318590.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="actin-5C"
                     /calculated_mol_wt=41707
     Region          7..371
                     /region_name="ASKHA_NBD_actin"
                     /note="nucleotide-binding domain (NBD) of actin and
                     similar proteins; cd10224"
                     /db_xref="CDD:466823"
     CDS             1..376
                     /gene="LOC117601604"
                     /coded_by="XM_034318590.2:178..1308"
                     /db_xref="GeneID:117601604"
ORIGIN      
        1 mcdeevaalv vdngsgmcka gfagddapra vfpsivgrpr hqgvmvgmgq kdsyvgdeaq
       61 skrgiltlky piehgivtnw ddmekiwhht fynelrvape ehpvllteap lnpkanrekm
      121 tqimfetfnt pamyvaiqav lslyasgrtt givldsgdgv shtvpiyegy alphailrld
      181 lagrdltdyl mkiltergys ftttaereiv rdikeklcyv aldfeqemat aassssleks
      241 yelpdgqvit ignerfrcpe alfqpsflgm eacgihetty nsimkcdvdi rkdlyantvl
      301 sggttmypgi adrmqkeits lapstmkiki iapperkysv wiggsilasl stfqqmwisk
      361 qeydesgpsi vhrkcf
//
LOCUS       XP_034174489             376 aa            linear   INV 03-SEP-2025
DEFINITION  actin-5C [Osmia lignaria lignaria].
ACCESSION   XP_034174489
VERSION     XP_034174489.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318598.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="actin-5C"
                     /calculated_mol_wt=41707
     Region          7..371
                     /region_name="ASKHA_NBD_actin"
                     /note="nucleotide-binding domain (NBD) of actin and
                     similar proteins; cd10224"
                     /db_xref="CDD:466823"
     CDS             1..376
                     /gene="LOC117601604"
                     /coded_by="XM_034318598.2:179..1309"
                     /db_xref="GeneID:117601604"
ORIGIN      
        1 mcdeevaalv vdngsgmcka gfagddapra vfpsivgrpr hqgvmvgmgq kdsyvgdeaq
       61 skrgiltlky piehgivtnw ddmekiwhht fynelrvape ehpvllteap lnpkanrekm
      121 tqimfetfnt pamyvaiqav lslyasgrtt givldsgdgv shtvpiyegy alphailrld
      181 lagrdltdyl mkiltergys ftttaereiv rdikeklcyv aldfeqemat aassssleks
      241 yelpdgqvit ignerfrcpe alfqpsflgm eacgihetty nsimkcdvdi rkdlyantvl
      301 sggttmypgi adrmqkeits lapstmkiki iapperkysv wiggsilasl stfqqmwisk
      361 qeydesgpsi vhrkcf
//
LOCUS       XP_034174503             208 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601616 [Osmia lignaria lignaria].
ACCESSION   XP_034174503
VERSION     XP_034174503.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318612.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="uncharacterized protein LOC117601616"
                     /calculated_mol_wt=24728
     Region          116..192
                     /region_name="HMG-box_NHP10-like"
                     /note="high mobility group (HMG)-box found in
                     Saccharomyces cerevisiae non-histone protein 10 (NHP10)
                     and similar proteins; cd22016"
                     /db_xref="CDD:438832"
     Site            order(123..124,126..130,133..134,141,156..157,160,
                     163..164,171,175,178,182,185)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438832"
     CDS             1..208
                     /gene="LOC117601616"
                     /coded_by="XM_034318612.2:41..667"
                     /db_xref="GeneID:117601616"
ORIGIN      
        1 msdeseydve peefdiykrk yqllldrcev lqqenerlvy riqrvrkllk rtrkekkfli
       61 drldqhgdrw reapmgvlee nstfqvtskp ektpkatghn skeektkkvt krkgtkadpn
      121 apkrpanpff qfcqeqrprv merlagepep skqeltrqla ttwkslssed kkvyydmyer
      181 skekyvaemq iynkksedtp nqmslnit
//
LOCUS       XP_034186862            1114 aa            linear   INV 03-SEP-2025
DEFINITION  CUB and Sushi multiple domains furrowed [Osmia lignaria lignaria].
ACCESSION   XP_034186862
VERSION     XP_034186862.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330971.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1114
                     /product="CUB and Sushi multiple domains furrowed"
                     /calculated_mol_wt=121510
     Region          22..77
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(32,51)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          78..220
                     /region_name="Sad1_UNC"
                     /note="Sad1 / UNC-like C-terminal; cl23730"
                     /db_xref="CDD:474037"
     Region          245..366
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(337,342,344,350,352..355,359..361)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Region          372..426
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(382,399)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          435..661
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          560..804
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          754..981
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     CDS             1..1114
                     /gene="fw"
                     /coded_by="XM_034330971.2:88..3432"
                     /db_xref="GeneID:117607354"
ORIGIN      
        1 mtreillsll gilvvgaegl kcghpavpmn akvslsdesl aigtlatytc dagyelfgsg
       61 sltcnargkw qgdlpfcgtn vafrkptnqs ttvrggdagh gndgdssteh dgkrctetqs
      121 epspwwkvdl lksysvkvvr vttrgccghq plqdieirvg nssvelqrnp lcawfpgtie
      181 egitktfqca raligqyvfl qlvgvegsls lcevevfavd efstdrcaya gtpadadlaa
      241 fnstcyefvv kkggsfqear nycrtrggdl vhgfkgassv yilnnlerrk dklktqlvwi
      301 gaqkeplita rtwrwvdgei vqkpswgkdq pnnyngeqnc vvldggrgwl wndvgcnldy
      361 lhwicqsrpp tcgspeksen ttiigskrti gstveyvcpd ghmligsknr tcelngfwsg
      421 eaptckfvdc gplaelenga itlvdkrtth galadyacke nytllgdarr rcgdggiwsg
      481 hqpqclfdwc peppqvnggl vtitgrrags tatyscqngf ilfgdnvltc diggewsgka
      541 pqcrfvdcga paqiefgsmt lingtttvks lavytcledy wlvgtakqec tkegkwshdt
      601 pscelitcee pdvpagsyvv gydlnvhssi eyhceagyql qgearhtcgr dgewsgevpt
      661 ceyvdcgkvl pvlngaadyv nktthlgsei tysctrnyrl ngvsrrycld ngqwsdatpk
      721 ceeircpepv yaehgilsvt gndrmygrtl irtgtpensn tgatsykiga lakyrcergy
      781 kvvgeplstc edngkwsgev prcvyvdcak pehiqhgkyt ltsnatyyga aalyecdgnf
      841 eldgfarrlc lengtwssdt pvckeirckd pekegvlstq vsthsvggva hyscprgyym
      901 egnetriclq ngswsgstpa cfsvdckhpg siengrvivv ngttiyggta eyhclpqyer
      961 vgpflrkcld tgswsgdepk celmpnevae aqgvgtsvgi gagviifili ilglvylrlr
     1021 katpvknten vqaaerkedq naavmsyatl ndgtpntmye nvpedglydn pysmsgstyg
     1081 ygsnmrrhyg rtghyeaepv snrngiting vsvr
//
LOCUS       XP_034186876             702 aa            linear   INV 03-SEP-2025
DEFINITION  protein bric-a-brac 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186876
VERSION     XP_034186876.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330985.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..702
                     /product="protein bric-a-brac 1 isoform X1"
                     /calculated_mol_wt=75394
     Region          37..120
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          392..437
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..702
                     /gene="LOC117607365"
                     /coded_by="XM_034330985.2:130..2238"
                     /db_xref="GeneID:117607365"
ORIGIN      
        1 mtqqsgagsp qqfclrwnny qtnltnvfdq llqsesfvdv tlacdghsvk ahkmvlsacs
       61 pyfqalffdn pcqhpivimk dikwpelkaa vefmykgein vsqeqigpll kvaeslkirg
      121 ladvnsehel tsrssleeaa naanaamhrk krrrisgers ppnsspdriv sgnladdqdg
      181 sssgagvimp dihsmlpsss tprsigspgt pnvsvtpqin lqelpvslpl ppppppppqg
      241 qqqsshsvsa hhvpghvtsg phasvnhlta hgqqltvqqq qqhqqqqqqq qqqqqqqqqq
      301 qqqqhhqqga agqpnpgddl eikpgiaemi reeeraklle sshawlgast ssiaadsyqy
      361 qlqsmwqkcw ntnqslvhnl rfrergplks wrpetmaeai fsvlkeglsl sqaarkydip
      421 yptfvlyanr vhnmlgpsad ggageylrpk grgrpqrill gvwpdehirg viravvfrdg
      481 hsphmvkeep tsmypslany aacngpegav spgaaaaaav aavaqglrhq mcsmvaaahs
      541 qqhdmmatnl stnlstslss nlqaamqasq hhhnstnsga agigsnnsgs gggagiggnn
      601 gnsplnlgls spgsggppes pmesplaspl gslmdpship ssvevgigvs gmtykparsf
      661 vsprpenlfq ediadlvkgp hglkdkdkip vklepltdsr ce
//
LOCUS       XP_034186886             701 aa            linear   INV 03-SEP-2025
DEFINITION  protein bric-a-brac 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186886
VERSION     XP_034186886.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330995.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..701
                     /product="protein bric-a-brac 1 isoform X2"
                     /calculated_mol_wt=75323
     Region          37..120
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          391..436
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..701
                     /gene="LOC117607365"
                     /coded_by="XM_034330995.2:130..2235"
                     /db_xref="GeneID:117607365"
ORIGIN      
        1 mtqqsgagsp qqfclrwnny qtnltnvfdq llqsesfvdv tlacdghsvk ahkmvlsacs
       61 pyfqalffdn pcqhpivimk dikwpelkaa vefmykgein vsqeqigpll kvaeslkirg
      121 ladvnsehel tsrssleeaa naanaamhrk krrrisgers ppnsspdriv sgnladdqdg
      181 sssgagvimp dihsmlpsss tprsigspgt pnvsvtpqin lqelpvslpl ppppppppqg
      241 qqqsshsvsa hhvpghvtsg phasvnhlta hgqqltvqqq qqhqqqqqqq qqqqqqqqqq
      301 qqqqhhqqga agqpnpgddl eikpgiaemi reeeraklle sshawlgast ssiadsyqyq
      361 lqsmwqkcwn tnqslvhnlr frergplksw rpetmaeaif svlkeglsls qaarkydipy
      421 ptfvlyanrv hnmlgpsadg gageylrpkg rgrpqrillg vwpdehirgv iravvfrdgh
      481 sphmvkeept smypslanya acngpegavs pgaaaaaava avaqglrhqm csmvaaahsq
      541 qhdmmatnls tnlstslssn lqaamqasqh hhnstnsgaa gigsnnsgsg ggagiggnng
      601 nsplnlglss pgsggppesp mesplasplg slmdpships svevgigvsg mtykparsfv
      661 sprpenlfqe diadlvkgph glkdkdkipv klepltdsrc e
//
LOCUS       XP_034186894             700 aa            linear   INV 03-SEP-2025
DEFINITION  protein bric-a-brac 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186894
VERSION     XP_034186894.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..700
                     /product="protein bric-a-brac 1 isoform X3"
                     /calculated_mol_wt=75159
     Region          37..120
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          392..437
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..700
                     /gene="LOC117607365"
                     /coded_by="XM_034331003.2:130..2232"
                     /db_xref="GeneID:117607365"
ORIGIN      
        1 mtqqsgagsp qqfclrwnny qtnltnvfdq llqsesfvdv tlacdghsvk ahkmvlsacs
       61 pyfqalffdn pcqhpivimk dikwpelkaa vefmykgein vsqeqigpll kvaeslkirg
      121 ladvnsehel tsrssleeaa naanaamhrk krrrisgers ppnsspdriv sgnladdqdg
      181 sssgagvimp dihsmlpsss tprsigspgt pnvsvtpqin lqelpvslpl ppppppppqg
      241 qqqsshsvsa hhvpghvtsg phasvnhlta hgqqltvqqq qqhqqqqqqq qqqqqqqqqq
      301 qqqqhhqqga agqpnpgddl eikpgiaemi reeeraklle sshawlgast ssiaadsyqy
      361 qlqsmwqkcw ntnqslvhnl rfrergplks wrpetmaeai fsvlkeglsl sqaarkydip
      421 yptfvlyanr vhnmlgpsad ggadlrpkgr grpqrillgv wpdehirgvi ravvfrdghs
      481 phmvkeepts mypslanyaa cngpegavsp gaaaaaavaa vaqglrhqmc smvaaahsqq
      541 hdmmatnlst nlstslssnl qaamqasqhh hnstnsgaag igsnnsgsgg gagiggnngn
      601 splnlglssp gsggppespm esplasplgs lmdpshipss vevgigvsgm tykparsfvs
      661 prpenlfqed iadlvkgphg lkdkdkipvk lepltdsrce
//
LOCUS       XP_034186902             699 aa            linear   INV 03-SEP-2025
DEFINITION  protein bric-a-brac 1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034186902
VERSION     XP_034186902.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331011.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..699
                     /product="protein bric-a-brac 1 isoform X4"
                     /calculated_mol_wt=75088
     Region          37..120
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          391..436
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..699
                     /gene="LOC117607365"
                     /coded_by="XM_034331011.2:130..2229"
                     /db_xref="GeneID:117607365"
ORIGIN      
        1 mtqqsgagsp qqfclrwnny qtnltnvfdq llqsesfvdv tlacdghsvk ahkmvlsacs
       61 pyfqalffdn pcqhpivimk dikwpelkaa vefmykgein vsqeqigpll kvaeslkirg
      121 ladvnsehel tsrssleeaa naanaamhrk krrrisgers ppnsspdriv sgnladdqdg
      181 sssgagvimp dihsmlpsss tprsigspgt pnvsvtpqin lqelpvslpl ppppppppqg
      241 qqqsshsvsa hhvpghvtsg phasvnhlta hgqqltvqqq qqhqqqqqqq qqqqqqqqqq
      301 qqqqhhqqga agqpnpgddl eikpgiaemi reeeraklle sshawlgast ssiadsyqyq
      361 lqsmwqkcwn tnqslvhnlr frergplksw rpetmaeaif svlkeglsls qaarkydipy
      421 ptfvlyanrv hnmlgpsadg gadlrpkgrg rpqrillgvw pdehirgvir avvfrdghsp
      481 hmvkeeptsm ypslanyaac ngpegavspg aaaaaavaav aqglrhqmcs mvaaahsqqh
      541 dmmatnlstn lstslssnlq aamqasqhhh nstnsgaagi gsnnsgsggg agiggnngns
      601 plnlglsspg sggppespme splasplgsl mdpshipssv evgigvsgmt ykparsfvsp
      661 rpenlfqedi adlvkgphgl kdkdkipvkl epltdsrce
//
LOCUS       XP_034186911             499 aa            linear   INV 03-SEP-2025
DEFINITION  protein bric-a-brac 1 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034186911
VERSION     XP_034186911.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..499
                     /product="protein bric-a-brac 1 isoform X5"
                     /calculated_mol_wt=55262
     Region          37..120
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          369..>402
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..499
                     /gene="LOC117607365"
                     /coded_by="XM_034331020.2:130..1629"
                     /db_xref="GeneID:117607365"
ORIGIN      
        1 mtqqsgagsp qqfclrwnny qtnltnvfdq llqsesfvdv tlacdghsvk ahkmvlsacs
       61 pyfqalffdn pcqhpivimk dikwpelkaa vefmykgein vsqeqigpll kvaeslkirg
      121 ladvnsehel tsrssleeaa naanaamhrk krrrisgers ppnsspdriv sgnladdqdg
      181 sssgagvimp dihsmlpsss tprsigspgt pnvsvtpqin lqelpvslpl ppppppppqg
      241 qqqsshsvsa hhvpghvtsg phasvnhlta hgqqltvqqq qqhqqqqqqq qqqqqqqqqq
      301 qqqqhhqqga agqpnpgddl eikpgiaemi reeeraklle sshawlgast ssiaadyssl
      361 lklkaptwtr hdlqeaieav vtqkmrftqa stkygipkgt lydnilgktk rmmvleeagl
      421 nsseetavld fccdisvspy nrrtkkslna ilnfveklrr krdpgfvfng lsgfrwwwaf
      481 ckkhsivsly fndenendq
//
LOCUS       XP_034186920             361 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607365 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186920
VERSION     XP_034186920.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331029.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..361
                     /product="uncharacterized protein LOC117607365 isoform X6"
                     /calculated_mol_wt=39523
     Region          37..120
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..361
                     /gene="LOC117607365"
                     /coded_by="XM_034331029.2:130..1215"
                     /db_xref="GeneID:117607365"
ORIGIN      
        1 mtqqsgagsp qqfclrwnny qtnltnvfdq llqsesfvdv tlacdghsvk ahkmvlsacs
       61 pyfqalffdn pcqhpivimk dikwpelkaa vefmykgein vsqeqigpll kvaeslkirg
      121 ladvnsehel tsrssleeaa naanaamhrk krrrisgers ppnsspdriv sgnladdqdg
      181 sssgagvimp dihsmlpsss tprsigspgt pnvsvtpqin lqelpvslpl ppppppppqg
      241 qqqsshsvsa hhvpghvtsg phasvnhlta hgqqltvqqq qqhqqqqqqq qqqqqqqqqq
      301 qqqqhhqqga agqpnpgddl eikpgiaemi reeervssrq wnanldqvsd rshankpspt
      361 r
//
LOCUS       XP_034186930             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607365 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186930
VERSION     XP_034186930.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331039.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117607365 isoform X7"
                     /calculated_mol_wt=37334
     Region          37..120
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..341
                     /gene="LOC117607365"
                     /coded_by="XM_034331039.2:130..1155"
                     /db_xref="GeneID:117607365"
ORIGIN      
        1 mtqqsgagsp qqfclrwnny qtnltnvfdq llqsesfvdv tlacdghsvk ahkmvlsacs
       61 pyfqalffdn pcqhpivimk dikwpelkaa vefmykgein vsqeqigpll kvaeslkirg
      121 ladvnsehel tsrssleeaa naanaamhrk krrrisgers ppnsspdriv sgnladdqdg
      181 sssgagvimp dihsmlpsss tprsigspgt pnvsvtpqin lqelpvslpl ppppppppqg
      241 qqqsshsvsa hhvpghvtsg phasvnhlta hgqqltvqqq qqhqqqqqqq qqqqqqqqqq
      301 qqqqhhqqga agqpnpgddl eikpgiaemi reeervstyh r
//
LOCUS       XP_076546233             628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546233
VERSION     XP_076546233.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690118.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="uncharacterized protein LOC117607396 isoform X1"
                     /calculated_mol_wt=68944
     Region          <352..484
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(359,361,365..366,369..371,373..374,378,381,391,393,
                     395,399..400,403..405,407..408,412,415,426,428,430,
                     434..435,438..440,442..443,447,450,459)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          359..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..628
                     /gene="LOC117607396"
                     /coded_by="XM_076690118.1:1370..3256"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqdh
      301 seadtivrkv psswetnneg rsallraard addaalneit lrvgkfgfgd mdvnvadssg
      361 rtaisymagn gtstmlelal sfdgvdpntp dnegntplhf aaqagqtdcl nillqrcpei
      421 evdarnasgf tplmkaalqg rtkcakillf aganptlrdh grglraeqwa rfcgryvcae
      481 vierfarhrl lerstscrwg sepelaakvl qgkvmpipst plsppssgfk skirkvfrtt
      541 sgpdrnfslv sqltsaalca sspalpksse vppvvksllr plsvpqlrvt lvspqdfldq
      601 skdkygsnfa ekientvakq ssrtkkkk
//
LOCUS       XP_034186941             628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186941
VERSION     XP_034186941.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331050.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186941.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="uncharacterized protein LOC117607396 isoform X1"
                     /calculated_mol_wt=68944
     Region          <352..484
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(359,361,365..366,369..371,373..374,378,381,391,393,
                     395,399..400,403..405,407..408,412,415,426,428,430,
                     434..435,438..440,442..443,447,450,459)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          359..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..628
                     /gene="LOC117607396"
                     /coded_by="XM_034331050.2:1576..3462"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqdh
      301 seadtivrkv psswetnneg rsallraard addaalneit lrvgkfgfgd mdvnvadssg
      361 rtaisymagn gtstmlelal sfdgvdpntp dnegntplhf aaqagqtdcl nillqrcpei
      421 evdarnasgf tplmkaalqg rtkcakillf aganptlrdh grglraeqwa rfcgryvcae
      481 vierfarhrl lerstscrwg sepelaakvl qgkvmpipst plsppssgfk skirkvfrtt
      541 sgpdrnfslv sqltsaalca sspalpksse vppvvksllr plsvpqlrvt lvspqdfldq
      601 skdkygsnfa ekientvakq ssrtkkkk
//
LOCUS       XP_034186949             628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186949
VERSION     XP_034186949.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331058.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186949.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="uncharacterized protein LOC117607396 isoform X1"
                     /calculated_mol_wt=68944
     Region          <352..484
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(359,361,365..366,369..371,373..374,378,381,391,393,
                     395,399..400,403..405,407..408,412,415,426,428,430,
                     434..435,438..440,442..443,447,450,459)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          359..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..628
                     /gene="LOC117607396"
                     /coded_by="XM_034331058.2:1555..3441"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqdh
      301 seadtivrkv psswetnneg rsallraard addaalneit lrvgkfgfgd mdvnvadssg
      361 rtaisymagn gtstmlelal sfdgvdpntp dnegntplhf aaqagqtdcl nillqrcpei
      421 evdarnasgf tplmkaalqg rtkcakillf aganptlrdh grglraeqwa rfcgryvcae
      481 vierfarhrl lerstscrwg sepelaakvl qgkvmpipst plsppssgfk skirkvfrtt
      541 sgpdrnfslv sqltsaalca sspalpksse vppvvksllr plsvpqlrvt lvspqdfldq
      601 skdkygsnfa ekientvakq ssrtkkkk
//
LOCUS       XP_034186959             628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186959
VERSION     XP_034186959.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331068.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186959.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="uncharacterized protein LOC117607396 isoform X1"
                     /calculated_mol_wt=68944
     Region          <352..484
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(359,361,365..366,369..371,373..374,378,381,391,393,
                     395,399..400,403..405,407..408,412,415,426,428,430,
                     434..435,438..440,442..443,447,450,459)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          359..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..628
                     /gene="LOC117607396"
                     /coded_by="XM_034331068.2:1304..3190"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqdh
      301 seadtivrkv psswetnneg rsallraard addaalneit lrvgkfgfgd mdvnvadssg
      361 rtaisymagn gtstmlelal sfdgvdpntp dnegntplhf aaqagqtdcl nillqrcpei
      421 evdarnasgf tplmkaalqg rtkcakillf aganptlrdh grglraeqwa rfcgryvcae
      481 vierfarhrl lerstscrwg sepelaakvl qgkvmpipst plsppssgfk skirkvfrtt
      541 sgpdrnfslv sqltsaalca sspalpksse vppvvksllr plsvpqlrvt lvspqdfldq
      601 skdkygsnfa ekientvakq ssrtkkkk
//
LOCUS       XP_034186969             628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186969
VERSION     XP_034186969.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331078.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186969.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="uncharacterized protein LOC117607396 isoform X1"
                     /calculated_mol_wt=68944
     Region          <352..484
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(359,361,365..366,369..371,373..374,378,381,391,393,
                     395,399..400,403..405,407..408,412,415,426,428,430,
                     434..435,438..440,442..443,447,450,459)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          359..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..628
                     /gene="LOC117607396"
                     /coded_by="XM_034331078.2:1037..2923"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqdh
      301 seadtivrkv psswetnneg rsallraard addaalneit lrvgkfgfgd mdvnvadssg
      361 rtaisymagn gtstmlelal sfdgvdpntp dnegntplhf aaqagqtdcl nillqrcpei
      421 evdarnasgf tplmkaalqg rtkcakillf aganptlrdh grglraeqwa rfcgryvcae
      481 vierfarhrl lerstscrwg sepelaakvl qgkvmpipst plsppssgfk skirkvfrtt
      541 sgpdrnfslv sqltsaalca sspalpksse vppvvksllr plsvpqlrvt lvspqdfldq
      601 skdkygsnfa ekientvakq ssrtkkkk
//
LOCUS       XP_034186977             628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186977
VERSION     XP_034186977.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331086.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186977.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="uncharacterized protein LOC117607396 isoform X1"
                     /calculated_mol_wt=68944
     Region          <352..484
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(359,361,365..366,369..371,373..374,378,381,391,393,
                     395,399..400,403..405,407..408,412,415,426,428,430,
                     434..435,438..440,442..443,447,450,459)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          359..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..628
                     /gene="LOC117607396"
                     /coded_by="XM_034331086.2:804..2690"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqdh
      301 seadtivrkv psswetnneg rsallraard addaalneit lrvgkfgfgd mdvnvadssg
      361 rtaisymagn gtstmlelal sfdgvdpntp dnegntplhf aaqagqtdcl nillqrcpei
      421 evdarnasgf tplmkaalqg rtkcakillf aganptlrdh grglraeqwa rfcgryvcae
      481 vierfarhrl lerstscrwg sepelaakvl qgkvmpipst plsppssgfk skirkvfrtt
      541 sgpdrnfslv sqltsaalca sspalpksse vppvvksllr plsvpqlrvt lvspqdfldq
      601 skdkygsnfa ekientvakq ssrtkkkk
//
LOCUS       XP_034186985             628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186985
VERSION     XP_034186985.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331094.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186985.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="uncharacterized protein LOC117607396 isoform X1"
                     /calculated_mol_wt=68944
     Region          <352..484
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(359,361,365..366,369..371,373..374,378,381,391,393,
                     395,399..400,403..405,407..408,412,415,426,428,430,
                     434..435,438..440,442..443,447,450,459)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          359..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          393..426
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          428..459
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..628
                     /gene="LOC117607396"
                     /coded_by="XM_034331094.2:851..2737"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqdh
      301 seadtivrkv psswetnneg rsallraard addaalneit lrvgkfgfgd mdvnvadssg
      361 rtaisymagn gtstmlelal sfdgvdpntp dnegntplhf aaqagqtdcl nillqrcpei
      421 evdarnasgf tplmkaalqg rtkcakillf aganptlrdh grglraeqwa rfcgryvcae
      481 vierfarhrl lerstscrwg sepelaakvl qgkvmpipst plsppssgfk skirkvfrtt
      541 sgpdrnfslv sqltsaalca sspalpksse vppvvksllr plsvpqlrvt lvspqdfldq
      601 skdkygsnfa ekientvakq ssrtkkkk
//
LOCUS       XP_034186989             522 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607396 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186989
VERSION     XP_034186989.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186989.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="uncharacterized protein LOC117607396 isoform X2"
                     /calculated_mol_wt=57734
     Region          <291..378
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          291..320
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(293..294,297..299,301..302,306,309,320,322,324,
                     328..329,332..334,336..337,341,344,353,355,358,362..363,
                     366..368,370..371,375)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          322..353
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..522
                     /gene="LOC117607396"
                     /coded_by="XM_034331098.2:1370..2938"
                     /db_xref="GeneID:117607396"
ORIGIN      
        1 mesgstmtkn hrdngltrqn etysrkdsft setkrnivdt aslvnvqrni sqgrgeivds
       61 swrvngvqas rikcsssvkg tgcivnnglv asrgelarns ctkrktpapc vvneksnavv
      121 dasyrakrpd rfleeeflgk rfsletarst tafrkphrai pksfslpddl regisaaqgk
      181 grrpfdngil krssaflani aqtacrvrek ktsdnrakss nvrgyftiln svtndfesly
      241 rspsvqtlpv erkkkkngvr vkksvsfssd tsfeekrapy rrtgavhevk ayhkgvlqgq
      301 tdclnillqr cpeievdarn asgftplmka alqgrtkcak illfaganpt lrdhgrglra
      361 eqwarfcgry vcaevierfa rhrllersts crwgsepela akvlqgkvmp ipstplspps
      421 sgfkskirkv frttsgpdrn fslvsqltsa alcasspalp kssevppvvk sllrplsvpq
      481 lrvtlvspqd fldqskdkyg snfaekient vakqssrtkk kk
//
LOCUS       XP_034187004             613 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607430 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187004
VERSION     XP_034187004.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331113.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187004.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..613
                     /product="uncharacterized protein LOC117607430 isoform X1"
                     /calculated_mol_wt=72739
     Region          172..196
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          204..307
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          420..>542
                     /region_name="U2AF_lg"
                     /note="U2 snRNP auxilliary factor, large subunit, splicing
                     factor; TIGR01642"
                     /db_xref="CDD:273727"
     CDS             1..613
                     /gene="LOC117607430"
                     /coded_by="XM_034331113.2:349..2190"
                     /db_xref="GeneID:117607430"
ORIGIN      
        1 mvmegccstk kshkewrrva kkerrrrlrr kaaverqade erlraaleqn adylnwlenc
       61 eleekekear ekeehdqrer ewleeevraq kewqilqerk rkarlvqleq eakirqefea
      121 kqeairkkqe eekrkreedi rkrelllkei ddyiddgikt pealrevies qpgkelcpff
      181 sktgacrygd tcsrnhrrvc lskvilvpgf ythfslekns aeydtdvale fetsetrqhf
      241 refykdvvpe lesfgriktl kyccntevhl rgnlyveyyt ereaaralrr lkgrwyagrq
      301 lncefvnlks wrsavcgmsk cpkgracnfl htfknphdey dikspprwak qqsvssshev
      361 astrrsdhkg kskwedgsdv eqsknwrwse spevevehrs rstdkrhrys ddrkstrdkr
      421 hepsdretsi khrrsssgkr leekskddkv sngrescrrr stkhqrkgee ehscdlpksp
      481 kysskrhsks sdksyrskip rsssnysrrd kedrdnvkhn sknhdkshed sprrksnels
      541 ekpntvdten tkyatrvksk wdkenfehid sesmesmdsd ssgsiddtks kvcskksdde
      601 wattdsedev kdk
//
LOCUS       XP_034187014             540 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607430 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187014
VERSION     XP_034187014.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331123.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187014.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="uncharacterized protein LOC117607430 isoform X2"
                     /calculated_mol_wt=63664
     Region          99..123
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          131..234
                     /region_name="RRM_U2AFBPL"
                     /note="RNA recognition motif (RRM) found in U2 small
                     nuclear ribonucleoprotein auxiliary factor 35 kDa
                     subunit-related protein 1 (U2AFBPL) and similar proteins;
                     cd12540"
                     /db_xref="CDD:409956"
     Region          347..>469
                     /region_name="U2AF_lg"
                     /note="U2 snRNP auxilliary factor, large subunit, splicing
                     factor; TIGR01642"
                     /db_xref="CDD:273727"
     CDS             1..540
                     /gene="LOC117607430"
                     /coded_by="XM_034331123.2:65..1687"
                     /db_xref="GeneID:117607430"
ORIGIN      
        1 mwipkpisyy mvpvraqkew qilqerkrka rlvqleqeak irqefeakqe airkkqeeek
       61 rkreedirkr elllkeiddy iddgiktpea lreviesqpg kelcpffskt gacrygdtcs
      121 rnhrrvclsk vilvpgfyth fsleknsaey dtdvalefet setrqhfref ykdvvpeles
      181 fgriktlkyc cntevhlrgn lyveyytere aaralrrlkg rwyagrqlnc efvnlkswrs
      241 avcgmskcpk gracnflhtf knphdeydik spprwakqqs vssshevast rrsdhkgksk
      301 wedgsdveqs knwrwsespe vevehrsrst dkrhrysddr kstrdkrhep sdretsikhr
      361 rsssgkrlee kskddkvsng rescrrrstk hqrkgeeehs cdlpkspkys skrhskssdk
      421 syrskiprss snysrrdked rdnvkhnskn hdkshedspr rksnelsekp ntvdtentky
      481 atrvkskwdk enfehidses mesmdsdssg siddtkskvc skksddewat tdsedevkdk
//
LOCUS       XP_034187026             600 aa            linear   INV 03-SEP-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034187026
VERSION     XP_034187026.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331135.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187026.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..600
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X1"
                     /calculated_mol_wt=67225
     Region          23..>60
                     /region_name="L27"
                     /note="L27 domain; cl02672"
                     /db_xref="CDD:470651"
     Region          81..130
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          143..232
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(153..154,164..168,179,182,212,216,219..220)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          245..304
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(247,271..272)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(250,252,255,266,284..285,302,304)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(401,407,424,431,434,443,466,471)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(401,407)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     Region          404..585
                     /region_name="GuKc"
                     /note="Guanylate kinase homologues; smart00072"
                     /db_xref="CDD:214504"
     CDS             1..600
                     /gene="metro"
                     /coded_by="XM_034331135.2:668..2470"
                     /db_xref="GeneID:117607436"
ORIGIN      
        1 mtaalvmena nwdaalskll nslqanknei psctdeefgf lsellqskel nalvnvhnki
       61 lnnvkddkff pvlsnsmdid idvlnllatk vhvskdckel fhllqkphiq gllcahdava
      121 qkdyyprlpe iplevdedee tvkivqlvks neplcgagve pivgatiktc evtgkiviar
      181 imhggaadrs glihvgdevc evngisvegk tpncvlkilq nsegtitfki vpadskggir
      241 eskvrvrahf sykasddpyi pckeagldfs kgdvlhivsq ddaywwqarr egdrnmragl
      301 ipsralqerr iilerqqkek tdddnislcs vpvplpalcp rpttslhasp tkcnlqgvkt
      361 kkimydaaen ddfdreeipt yeevaklypr pglyrpvvli gppgvgrnel krrlmatdsd
      421 kyktpvpyts rppksgeing keyhfvtrek meeeieagkf ieygeykgnl ygtssesvss
      481 linagyvcll nphyqalkml rtpqtkpyvi yikpprfeil ketrndarar stfdesnsrg
      541 ftdeefneil hsaariefly shlfdeaivn adlsmafeql vnavhrvese plwvpaswvq
//
LOCUS       XP_034187034             594 aa            linear   INV 03-SEP-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034187034
VERSION     XP_034187034.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187034.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..594
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X2"
                     /calculated_mol_wt=66670
     Region          23..>60
                     /region_name="L27"
                     /note="L27 domain; cl02672"
                     /db_xref="CDD:470651"
     Region          81..130
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          143..232
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(153..154,164..168,179,182,212,216,219..220)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          245..304
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(247,271..272)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(250,252,255,266,284..285,302,304)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(395,401,418,425,428,437,460,465)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(395,401)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     Region          398..579
                     /region_name="GuKc"
                     /note="Guanylate kinase homologues; smart00072"
                     /db_xref="CDD:214504"
     CDS             1..594
                     /gene="metro"
                     /coded_by="XM_034331143.2:733..2517"
                     /db_xref="GeneID:117607436"
ORIGIN      
        1 mtaalvmena nwdaalskll nslqanknei psctdeefgf lsellqskel nalvnvhnki
       61 lnnvkddkff pvlsnsmdid idvlnllatk vhvskdckel fhllqkphiq gllcahdava
      121 qkdyyprlpe iplevdedee tvkivqlvks neplcgagve pivgatiktc evtgkiviar
      181 imhggaadrs glihvgdevc evngisvegk tpncvlkilq nsegtitfki vpadskggir
      241 eskvrvrahf sykasddpyi pckeagldfs kgdvlhivsq ddaywwqarr egdrnmragl
      301 ipsralqerr iilerqqkek tdddnimplp alcprpttsl hasptkcnlq gvktkkimyd
      361 aaenddfdre eiptyeevak lyprpglyrp vvligppgvg rnelkrrlma tdsdkyktpv
      421 pytsrppksg eingkeyhfv trekmeeeie agkfieygey kgnlygtsse svsslinagy
      481 vcllnphyqa lkmlrtpqtk pyviyikppr feilketrnd ararstfdes nsrgftdeef
      541 neilhsaari eflyshlfde aivnadlsma feqlvnavhr veseplwvpa swvq
//
LOCUS       XP_034187044             591 aa            linear   INV 03-SEP-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034187044
VERSION     XP_034187044.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331153.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187044.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X3"
                     /calculated_mol_wt=66399
     Region          23..>60
                     /region_name="L27"
                     /note="L27 domain; cl02672"
                     /db_xref="CDD:470651"
     Region          81..130
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          143..223
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(153..159,170,173,203,207,210..211)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          236..295
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(238,262..263)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(241,243,246,257,275..276,293,295)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(392,398,415,422,425,434,457,462)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(392,398)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     Region          395..576
                     /region_name="GuKc"
                     /note="Guanylate kinase homologues; smart00072"
                     /db_xref="CDD:214504"
     CDS             1..591
                     /gene="metro"
                     /coded_by="XM_034331153.2:609..2384"
                     /db_xref="GeneID:117607436"
ORIGIN      
        1 mtaalvmena nwdaalskll nslqanknei psctdeefgf lsellqskel nalvnvhnki
       61 lnnvkddkff pvlsnsmdid idvlnllatk vhvskdckel fhllqkphiq gllcahdava
      121 qkdyyprlpe iplevdedee tvkivqlvks neplgatikt cevtgkivia rimhggaadr
      181 sglihvgdev cevngisveg ktpncvlkil qnsegtitfk ivpadskggi reskvrvrah
      241 fsykasddpy ipckeagldf skgdvlhivs qddaywwqar regdrnmrag lipsralqer
      301 riilerqqke ktdddnislc svpvplpalc prpttslhas ptkcnlqgvk tkkimydaae
      361 nddfdreeip tyeevaklyp rpglyrpvvl igppgvgrne lkrrlmatds dkyktpvpyt
      421 srppksgein gkeyhfvtre kmeeeieagk fieygeykgn lygtssesvs slinagyvcl
      481 lnphyqalkm lrtpqtkpyv iyikpprfei lketrndara rstfdesnsr gftdeefnei
      541 lhsaariefl yshlfdeaiv nadlsmafeq lvnavhrves eplwvpaswv q
//
LOCUS       XP_034187052             558 aa            linear   INV 03-SEP-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034187052
VERSION     XP_034187052.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331161.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187052.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..558
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X4"
                     /calculated_mol_wt=62804
     Region          23..>60
                     /region_name="L27"
                     /note="L27 domain; cl02672"
                     /db_xref="CDD:470651"
     Region          81..130
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          143..232
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(153..154,164..168,179,182,212,216,219..220)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          245..304
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(247,271..272)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(250,252,255,266,284..285,302,304)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(359,365,382,389,392,401,424,429)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(359,365)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     Region          362..543
                     /region_name="GuKc"
                     /note="Guanylate kinase homologues; smart00072"
                     /db_xref="CDD:214504"
     CDS             1..558
                     /gene="metro"
                     /coded_by="XM_034331161.2:660..2336"
                     /db_xref="GeneID:117607436"
ORIGIN      
        1 mtaalvmena nwdaalskll nslqanknei psctdeefgf lsellqskel nalvnvhnki
       61 lnnvkddkff pvlsnsmdid idvlnllatk vhvskdckel fhllqkphiq gllcahdava
      121 qkdyyprlpe iplevdedee tvkivqlvks neplcgagve pivgatiktc evtgkiviar
      181 imhggaadrs glihvgdevc evngisvegk tpncvlkilq nsegtitfki vpadskggir
      241 eskvrvrahf sykasddpyi pckeagldfs kgdvlhivsq ddaywwqarr egdrnmragl
      301 ipsralqerr iilerqqkek tdddnikndd fdreeiptye evaklyprpg lyrpvvligp
      361 pgvgrnelkr rlmatdsdky ktpvpytsrp pksgeingke yhfvtrekme eeieagkfie
      421 ygeykgnlyg tssesvssli nagyvcllnp hyqalkmlrt pqtkpyviyi kpprfeilke
      481 trndararst fdesnsrgft deefneilhs aarieflysh lfdeaivnad lsmafeqlvn
      541 avhrvesepl wvpaswvq
//
LOCUS       XP_034187063             549 aa            linear   INV 03-SEP-2025
DEFINITION  membrane palmitoylated protein 7-like protein metro isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034187063
VERSION     XP_034187063.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187063.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..549
                     /product="membrane palmitoylated protein 7-like protein
                     metro isoform X5"
                     /calculated_mol_wt=61978
     Region          23..>60
                     /region_name="L27"
                     /note="L27 domain; cl02672"
                     /db_xref="CDD:470651"
     Region          81..130
                     /region_name="L27"
                     /note="domain in receptor targeting proteins Lin-2 and
                     Lin-7; smart00569"
                     /db_xref="CDD:197794"
     Region          143..223
                     /region_name="PDZ_MPP3-MPP4-MPP7-like"
                     /note="PDZ domain of membrane palmitoylated proteins 3
                     (MPP3), MPP4, and MPP7, and related domains; cd06799"
                     /db_xref="CDD:467260"
     Site            order(153..159,170,173,203,207,210..211)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467260"
     Region          236..295
                     /region_name="SH3_MPP"
                     /note="Src Homology 3 domain of Membrane Protein,
                     Palmitoylated (or MAGUK p55 subfamily member) proteins;
                     cd11862"
                     /db_xref="CDD:212796"
     Site            order(238,262..263)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(241,243,246,257,275..276,293,295)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212796"
     Site            order(350,356,373,380,383,392,415,420)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:238026"
     Site            order(350,356)
                     /site_type="other"
                     /note="G-X2-G-X-G-K"
                     /db_xref="CDD:238026"
     Region          353..534
                     /region_name="GuKc"
                     /note="Guanylate kinase homologues; smart00072"
                     /db_xref="CDD:214504"
     CDS             1..549
                     /gene="metro"
                     /coded_by="XM_034331172.2:590..2239"
                     /db_xref="GeneID:117607436"
ORIGIN      
        1 mtaalvmena nwdaalskll nslqanknei psctdeefgf lsellqskel nalvnvhnki
       61 lnnvkddkff pvlsnsmdid idvlnllatk vhvskdckel fhllqkphiq gllcahdava
      121 qkdyyprlpe iplevdedee tvkivqlvks neplgatikt cevtgkivia rimhggaadr
      181 sglihvgdev cevngisveg ktpncvlkil qnsegtitfk ivpadskggi reskvrvrah
      241 fsykasddpy ipckeagldf skgdvlhivs qddaywwqar regdrnmrag lipsralqer
      301 riilerqqke ktdddniknd dfdreeipty eevaklyprp glyrpvvlig ppgvgrnelk
      361 rrlmatdsdk yktpvpytsr ppksgeingk eyhfvtrekm eeeieagkfi eygeykgnly
      421 gtssesvssl inagyvclln phyqalkmlr tpqtkpyviy ikpprfeilk etrndarars
      481 tfdesnsrgf tdeefneilh saarieflys hlfdeaivna dlsmafeqlv navhrvesep
      541 lwvpaswvq
//
LOCUS       XP_034187075             585 aa            linear   INV 03-SEP-2025
DEFINITION  protein GPR107 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034187075
VERSION     XP_034187075.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331184.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="protein GPR107 isoform X1"
                     /calculated_mol_wt=67502
     Region          223..511
                     /region_name="Lung_7-TM_R"
                     /note="Lung seven transmembrane receptor; pfam06814"
                     /db_xref="CDD:369089"
     CDS             1..585
                     /gene="LOC117607462"
                     /coded_by="XM_034331184.2:113..1870"
                     /db_xref="GeneID:117607462"
ORIGIN      
        1 mrskqlliqc lwimsvlvla sgrihkleir kdvrryisls tfgfykggil dvnltnfkae
       61 pfhenavfgf sldrtlsdam npyldshqer cvlqdisnvk sdfeqrddsa iiyftmdlkn
      121 nllkvncsrn vhtvhiykds ssvlafrekr nsfpgrfsdt vlfprrkryt sygiersave
      181 defetksses nvcsrlklpm tvqtikgeky yntsfavyva skeeeglynl yfhncpnyky
      241 dspvaldfti qiseinsgnf lsagemplpa lyfmmallff lsgcfwvfil rkskhpvfki
      301 hylmailvyl kslsllfhgi nyhfiqtkge hvaawailyy ithllkgavl fitivlvgtg
      361 wtfikhilad kdkklfmivi plqvlanvae iiieeseegd iehktwrdvf ilvdllccga
      421 ilfpvvwsir hlqeaahtdg kaavnlrklk lfrhfyimiv cyiyftriiv yllkitvpfq
      481 yewldemfre matyvffvlt gykfrpasan pyftltndet tqadeddemd vvlfssisgr
      541 sgiteglskv skvpkvtrpa tsdvpstqee rdclfnktep sheyd
//
LOCUS       XP_034187084             581 aa            linear   INV 03-SEP-2025
DEFINITION  protein GPR107 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034187084
VERSION     XP_034187084.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="protein GPR107 isoform X2"
                     /calculated_mol_wt=67068
     Region          223..511
                     /region_name="Lung_7-TM_R"
                     /note="Lung seven transmembrane receptor; pfam06814"
                     /db_xref="CDD:369089"
     CDS             1..581
                     /gene="LOC117607462"
                     /coded_by="XM_034331193.2:113..1858"
                     /db_xref="GeneID:117607462"
ORIGIN      
        1 mrskqlliqc lwimsvlvla sgrihkleir kdvrryisls tfgfykggil dvnltnfkae
       61 pfhenavfgf sldrtlsdam npyldshqer cvlqdisnvk sdfeqrddsa iiyftmdlkn
      121 nllkvncsrn vhtvhiykds ssvlafrekr nsfpgrfsdt vlfprrkryt sygiersave
      181 defetksses nvcsrlklpm tvqtikgeky yntsfavyva skeeeglynl yfhncpnyky
      241 dspvaldfti qiseinsgnf lsagemplpa lyfmmallff lsgcfwvfil rkskhpvfki
      301 hylmailvyl kslsllfhgi nyhfiqtkge hvaawailyy ithllkgavl fitivlvgtg
      361 wtfikhilad kdkklfmivi plqvlanvae iiieeseegd iehktwrdvf ilvdllccga
      421 ilfpvvwsir hlqeaahtdg kaavnlrklk lfrhfyimiv cyiyftriiv yllkitvpfq
      481 yewldemfre matyvffvlt gykfrpasan pyftltndet tqadeddemd vvisgrsgit
      541 eglskvskvp kvtrpatsdv pstqeerdcl fnktepshey d
//
LOCUS       XP_034187095             558 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607476 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187095
VERSION     XP_034187095.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331204.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..558
                     /product="uncharacterized protein LOC117607476 isoform X1"
                     /calculated_mol_wt=63281
     Region          445..556
                     /region_name="hdxy_isourate"
                     /note="hydroxyisourate hydrolase; TIGR02962"
                     /db_xref="CDD:274364"
     Site            order(450,452,488,540,542,544,553)
                     /site_type="active"
                     /db_xref="CDD:100114"
     Site            order(454..457,522..523,525,529..531,546,548..554)
                     /site_type="other"
                     /note="homotetramer interface [polypeptide binding]"
                     /db_xref="CDD:100114"
     CDS             1..558
                     /gene="LOC117607476"
                     /coded_by="XM_034331204.2:148..1824"
                     /db_xref="GeneID:117607476"
ORIGIN      
        1 meqkwqspii tksetvlnts fltatgqrlt ptgkishakt rvytqryrke weqmpdfkgw
       61 ltsvpfqptr ayclyckknl hahrlsllkh tctmkhqraa llheaeekkk aaarnasmge
      121 dveveevdei eaveyaktei eenedeveyv verletddel detqikdpne dvggkieeee
      181 eeeeeeevth iqvpsdeedv khsmkkikie rvkqcedslt eamdhmqsey leeadnqdtv
      241 qlemvvesed qsnpemevms ilpdtedstk eeslkesren eravrrgdnk ldkktgkllq
      301 deckqhgdsg ivmacplpil gttyqintst aptsntigvl qpmntipiap aqnktitlts
      361 ggktltltgg tfqpgtqyvl sklknklptl imadkkptia anqvedatkg vqinkdqmav
      421 astsyqnvkk hvllknfksp likkpristh vmdtskglpv gglqvslykl mdgrwtflne
      481 sntspngrcv dlvdnmkhft agrykihfdv dkyftlrrie tmypfieivf dvknpaghyh
      541 ipvllspfgy ttyrgser
//
LOCUS       XP_034187104             558 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607476 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187104
VERSION     XP_034187104.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331213.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..558
                     /product="uncharacterized protein LOC117607476 isoform X1"
                     /calculated_mol_wt=63281
     Region          445..556
                     /region_name="hdxy_isourate"
                     /note="hydroxyisourate hydrolase; TIGR02962"
                     /db_xref="CDD:274364"
     Site            order(450,452,488,540,542,544,553)
                     /site_type="active"
                     /db_xref="CDD:100114"
     Site            order(454..457,522..523,525,529..531,546,548..554)
                     /site_type="other"
                     /note="homotetramer interface [polypeptide binding]"
                     /db_xref="CDD:100114"
     CDS             1..558
                     /gene="LOC117607476"
                     /coded_by="XM_034331213.2:148..1824"
                     /db_xref="GeneID:117607476"
ORIGIN      
        1 meqkwqspii tksetvlnts fltatgqrlt ptgkishakt rvytqryrke weqmpdfkgw
       61 ltsvpfqptr ayclyckknl hahrlsllkh tctmkhqraa llheaeekkk aaarnasmge
      121 dveveevdei eaveyaktei eenedeveyv verletddel detqikdpne dvggkieeee
      181 eeeeeeevth iqvpsdeedv khsmkkikie rvkqcedslt eamdhmqsey leeadnqdtv
      241 qlemvvesed qsnpemevms ilpdtedstk eeslkesren eravrrgdnk ldkktgkllq
      301 deckqhgdsg ivmacplpil gttyqintst aptsntigvl qpmntipiap aqnktitlts
      361 ggktltltgg tfqpgtqyvl sklknklptl imadkkptia anqvedatkg vqinkdqmav
      421 astsyqnvkk hvllknfksp likkpristh vmdtskglpv gglqvslykl mdgrwtflne
      481 sntspngrcv dlvdnmkhft agrykihfdv dkyftlrrie tmypfieivf dvknpaghyh
      541 ipvllspfgy ttyrgser
//
LOCUS       XP_034187114             557 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607476 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187114
VERSION     XP_034187114.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331223.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="uncharacterized protein LOC117607476 isoform X2"
                     /calculated_mol_wt=63153
     Region          444..555
                     /region_name="hdxy_isourate"
                     /note="hydroxyisourate hydrolase; TIGR02962"
                     /db_xref="CDD:274364"
     Site            order(449,451,487,539,541,543,552)
                     /site_type="active"
                     /db_xref="CDD:100114"
     Site            order(453..456,521..522,524,528..530,545,547..553)
                     /site_type="other"
                     /note="homotetramer interface [polypeptide binding]"
                     /db_xref="CDD:100114"
     CDS             1..557
                     /gene="LOC117607476"
                     /coded_by="XM_034331223.2:148..1821"
                     /db_xref="GeneID:117607476"
ORIGIN      
        1 meqkwqspii tksetvlnts fltatgqrlt ptgkishakt rvytqryrke weqmpdfkgw
       61 ltsvpfqptr ayclyckknl hahrlsllkh tctmkhqraa llheaeekkk aaarnasmge
      121 dveveevdei eaveyaktei eenedeveyv verletddel detqikdpne dvggkieeee
      181 eeeeeeevth iqvpsdeedv khsmkkikie rvqcedslte amdhmqseyl eeadnqdtvq
      241 lemvvesedq snpemevmsi lpdtedstke eslkesrene ravrrgdnkl dkktgkllqd
      301 eckqhgdsgi vmacplpilg ttyqintsta ptsntigvlq pmntipiapa qnktitltsg
      361 gktltltggt fqpgtqyvls klknklptli madkkptiaa nqvedatkgv qinkdqmava
      421 stsyqnvkkh vllknfkspl ikkpristhv mdtskglpvg glqvslyklm dgrwtflnes
      481 ntspngrcvd lvdnmkhfta grykihfdvd kyftlrriet mypfieivfd vknpaghyhi
      541 pvllspfgyt tyrgser
//
LOCUS       XP_034187124             465 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607476 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187124
VERSION     XP_034187124.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331233.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..465
                     /product="uncharacterized protein LOC117607476 isoform X3"
                     /calculated_mol_wt=52355
     Region          352..463
                     /region_name="hdxy_isourate"
                     /note="hydroxyisourate hydrolase; TIGR02962"
                     /db_xref="CDD:274364"
     Site            order(357,359,395,447,449,451,460)
                     /site_type="active"
                     /db_xref="CDD:100114"
     Site            order(361..364,429..430,432,436..438,453,455..461)
                     /site_type="other"
                     /note="homotetramer interface [polypeptide binding]"
                     /db_xref="CDD:100114"
     CDS             1..465
                     /gene="LOC117607476"
                     /coded_by="XM_034331233.2:222..1619"
                     /db_xref="GeneID:117607476"
ORIGIN      
        1 mkhqraallh eaeekkkaaa rnasmgedve veevdeieav eyakteieen edeveyvver
       61 letddeldet qikdpnedvg gkieeeeeee eeeevthiqv psdeedvkhs mkkikiervk
      121 qcedslteam dhmqseylee adnqdtvqle mvvesedqsn pemevmsilp dtedstkees
      181 lkesrenera vrrgdnkldk ktgkllqdec kqhgdsgivm acplpilgtt yqintstapt
      241 sntigvlqpm ntipiapaqn ktitltsggk tltltggtfq pgtqyvlskl knklptlima
      301 dkkptiaanq vedatkgvqi nkdqmavast syqnvkkhvl lknfksplik kpristhvmd
      361 tskglpvggl qvslyklmdg rwtflnesnt spngrcvdlv dnmkhftagr ykihfdvdky
      421 ftlrrietmy pfieivfdvk npaghyhipv llspfgytty rgser
//
LOCUS       XP_034187133             465 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607476 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187133
VERSION     XP_034187133.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331242.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..465
                     /product="uncharacterized protein LOC117607476 isoform X3"
                     /calculated_mol_wt=52355
     Region          352..463
                     /region_name="hdxy_isourate"
                     /note="hydroxyisourate hydrolase; TIGR02962"
                     /db_xref="CDD:274364"
     Site            order(357,359,395,447,449,451,460)
                     /site_type="active"
                     /db_xref="CDD:100114"
     Site            order(361..364,429..430,432,436..438,453,455..461)
                     /site_type="other"
                     /note="homotetramer interface [polypeptide binding]"
                     /db_xref="CDD:100114"
     CDS             1..465
                     /gene="LOC117607476"
                     /coded_by="XM_034331242.2:252..1649"
                     /db_xref="GeneID:117607476"
ORIGIN      
        1 mkhqraallh eaeekkkaaa rnasmgedve veevdeieav eyakteieen edeveyvver
       61 letddeldet qikdpnedvg gkieeeeeee eeeevthiqv psdeedvkhs mkkikiervk
      121 qcedslteam dhmqseylee adnqdtvqle mvvesedqsn pemevmsilp dtedstkees
      181 lkesrenera vrrgdnkldk ktgkllqdec kqhgdsgivm acplpilgtt yqintstapt
      241 sntigvlqpm ntipiapaqn ktitltsggk tltltggtfq pgtqyvlskl knklptlima
      301 dkkptiaanq vedatkgvqi nkdqmavast syqnvkkhvl lknfksplik kpristhvmd
      361 tskglpvggl qvslyklmdg rwtflnesnt spngrcvdlv dnmkhftagr ykihfdvdky
      421 ftlrrietmy pfieivfdvk npaghyhipv llspfgytty rgser
//
LOCUS       XP_034196651             556 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612067 [Osmia lignaria lignaria].
ACCESSION   XP_034196651
VERSION     XP_034196651.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..556
                     /product="uncharacterized protein LOC117612067"
                     /calculated_mol_wt=64762
     Region          <139..>210
                     /region_name="RNase_Y_N"
                     /note="RNase Y N-terminal region; pfam12072"
                     /db_xref="CDD:463456"
     CDS             1..556
                     /gene="LOC117612067"
                     /coded_by="XM_034340760.2:2300..3970"
                     /db_xref="GeneID:117612067"
ORIGIN      
        1 mcfhasiali kmdsdsvqik lgerddisds kkckcdciee nmqnlrncvt ientveanya
       61 ninqpvgdtt nsfynlsgic ktkyeemcqe hnvhyqnkih kihkltpine qnedckdsit
      121 dlcyhgckng csatvptpqe vsekvfkenw lqkledikqk etvlrnkeit lqnrekallk
      181 keretkiles lwkhklkqld lyfkenkdlq ylltdystqk nnsesannmh snklhkeevc
      241 indnfpenid sidvneqnfr tqnsstqhll ynkteqfkkh tdnigimykg lhhekinils
      301 ssclpinnvs vakkgknqsd lrssiaakss yskstyrdnn ktkksskidy ndldttlsad
      361 igdssfvqts qkfnpeiyrk pymftrsase rhikyinhks npnleaqnln ypdeksetlt
      421 giaqgkvler vtknivasrd rstkfqhygy mdqnmdsvnk mcrveneriy sylnletgnk
      481 lcscqklnkd lkdrpvswne etnewlqkkr kaynmmmnkt htenkenfey ntkmnsklet
      541 gvkkkdiknr iftifr
//
LOCUS       XP_034187144             548 aa            linear   INV 03-SEP-2025
DEFINITION  nucleobindin 1 [Osmia lignaria lignaria].
ACCESSION   XP_034187144
VERSION     XP_034187144.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331253.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187144.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..548
                     /product="nucleobindin 1"
                     /calculated_mol_wt=64535
     Region          240..306
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(240,242,244,251,292,294,296,303)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     CDS             1..548
                     /gene="NUCB1"
                     /coded_by="XM_034331253.2:146..1792"
                     /db_xref="GeneID:117607494"
ORIGIN      
        1 mkyfllfllt avtvqlsvap pvnqkskdge hisenkvedv eemedvmeyr ryltevvqvl
       61 esdpdfrtkl qeadeadirt gkiadqlqfv shnirtrlde ikreelerlr hlatkqnelr
      121 ngidtehldl knthtfeiqd lkkliaktte dlakadrkrr eefkqhemer kyeeeeklkh
      181 mteverkkye qelqamrekq khhetvnhpg skhqleevwe kqdqmateef npktfffyhd
      241 vdgngvwdqd evkalflqel dklydkgtpq adllkraeem ermrehvfne adlnkdglis
      301 yeefleqtkk pdfqqdegwv gldeqqvyne eefeafrrhk qeemirqgml pehpgnipvq
      361 qepfppqygh vpahgvvppq vdvggpsqqn lpqqqfqnhp pqyyqqqgni vtqhhypnha
      421 pvqpihdqip qqqqqqqqqq qhqqqqqhqq qqqqfhgqip vnqqqmegqv pvqqnqasvq
      481 qqqipvqphq pqasnfqqqh nivnqvpqnv qnnavpslnd pnqlnlqpvq pavqqdvnrn
      541 iipsveki
//
LOCUS       XP_034187157             478 aa            linear   INV 03-SEP-2025
DEFINITION  putative serine protease K12H4.7 [Osmia lignaria lignaria].
ACCESSION   XP_034187157
VERSION     XP_034187157.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331266.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187157.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="putative serine protease K12H4.7"
                     /calculated_mol_wt=54448
     Region          51..468
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..478
                     /gene="LOC117607499"
                     /coded_by="XM_034331266.2:103..1539"
                     /db_xref="GeneID:117607499"
ORIGIN      
        1 miflsvllsl ciafrasdav glhgfhfrgl eepfslnrsd sqnviearie qpldhfnprd
       61 nrtwsmryye ntglhkkggp imimiggewe istgflksgl mfeiasahna mmyytehryy
      121 gqskptgdis ssnlqylsvd qaladlayfi eikkkdealr nstvivfggs yagsmaawar
      181 lkyphliqga lassapmyak adfyeyyevv teslrrhsen cadevkvafn sveellfskd
      241 gpeklktyfh lcdlpdvksp ndighlmnil aeefagivqy dkveknrtki aaycekmtak
      301 ylgsplqrla hlvsdnltdc vennyqkfvk lyknetwssk inrqwyyqtc tefgyyqttn
      361 snnsvfgslf plhyftdmct dlygnyynnd flntrivrtn lmyggvrpdl vkviftngdv
      421 dpwhklsvlq dlnayspail ikgsshcrdl ysdldtdvkd lvnaranvrk iigtwlas
//
LOCUS       XP_034187170             462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607509 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187170
VERSION     XP_034187170.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331279.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187170.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="uncharacterized protein LOC117607509 isoform X1"
                     /calculated_mol_wt=52700
     Region          10..75
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..462
                     /gene="LOC117607509"
                     /coded_by="XM_034331279.2:225..1613"
                     /db_xref="GeneID:117607509"
ORIGIN      
        1 msrnlseter kknqfdkeer vhllaimkqy aplldtstst larkriwtii eeefkkagft
       61 rktsaqlkky wqnykyhckk akvlgkeskr crnsgdnesv ewnryqifvd nqavtkfpev
      121 seslrsmsdr lqssstemyq hdgsstgkln tsdasidlsr vkmerndeet msnycdmeet
      181 nplkdlslee dsldekkeif mnedqlvsps tttakhktrn rivvsnsris nnsvtvsviy
      241 pearnetdfl sisksaityi cicilaevsw neckeksndl nlsenpnaiy paplvdcpnl
      301 feydndpgnl drtngsaitd nekihlfghn gsenfihakq mdddfdctgg kndvtvrgkc
      361 pktrqndasr srgcvfltdy rnrlrhrlll qqleteekrl kvkiaemtfq evqlrikaly
      421 edmrrteelh rlrllqaaas aaasaaasta tttigngktn nv
//
LOCUS       XP_034187180             452 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607509 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187180
VERSION     XP_034187180.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331289.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187180.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="uncharacterized protein LOC117607509 isoform X2"
                     /calculated_mol_wt=51593
     Region          10..75
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     CDS             1..452
                     /gene="LOC117607509"
                     /coded_by="XM_034331289.2:221..1579"
                     /db_xref="GeneID:117607509"
ORIGIN      
        1 msrnlseter kknqfdkeer vhllaimkqy aplldtstst larkriwtii eeefkkagft
       61 rktsaqlkky wqnykyhckk akvlgkeskr crnsgdnesv ewnryqifvd nqavtkfpev
      121 seslrsmsdr lqssstemyq hdgsstgkln tsdasidlsr vkmerndeet msnycdmeet
      181 nplkdlslee dsldekkeif mnedqlvsps tttakhktrn rivvsnsris nnsvtvsviy
      241 pearnetdfl sisksaevsw neckeksndl nlsenpnaiy paplvdcpnl feydndpgnl
      301 drtngsaitd nekihlfghn gsenfihakq mdddfdctgg kndvtvrgkc pktrqndasr
      361 srgcvfltdy rnrlrhrlll qqleteekrl kvkiaemtfq evqlrikaly edmrrteelh
      421 rlrllqaaas aaasaaasta tttigngktn nv
//
LOCUS       XP_034187193             449 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607519 [Osmia lignaria lignaria].
ACCESSION   XP_034187193
VERSION     XP_034187193.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331302.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="uncharacterized protein LOC117607519"
                     /calculated_mol_wt=49506
     Region          10..131
                     /region_name="DUF4745"
                     /note="Domain of unknown function (DUF4745); pfam15923"
                     /db_xref="CDD:374226"
     CDS             1..449
                     /gene="LOC117607519"
                     /coded_by="XM_034331302.2:616..1965"
                     /db_xref="GeneID:117607519"
ORIGIN      
        1 maeggtepks ndnqvskhql evneclgnwl vylqmlnglc tagtklaqsl qtllsvhdtv
       61 aqcrltgqcl agweeltrat yiasntvrnh iiaalrdhet reseadkhdi lrdnlltfin
      121 lqyqfcvacc eclggmaecs csqsgaaecd iaslqqcfer lyssplpvss sstqqsvqnc
      181 hrsplpyplf plqvqrrwse taaaemsgev senpirrwsm pwdcrnitdw prqdvrsrlr
      241 vphqdrsrst tpdtiwktst masqdglqea iqllsckpgv rplnqltayt nqhipgvtlt
      301 tcnfetnyds tiwpgsrkvg ssrcwphdsh ssdhsdqsgr resdqsahsg ehrdseqsga
      361 sgshgdskds ihshsdhrev epgtvdvlps rksssstdsc isahsrsgse sagggecars
      421 qlysmwsgsd lpfiklpesn etqdehppv
//
LOCUS       XP_034187205             446 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607525 [Osmia lignaria lignaria].
ACCESSION   XP_034187205
VERSION     XP_034187205.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331314.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187205.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="uncharacterized protein LOC117607525"
                     /calculated_mol_wt=50704
     Region          265..>297
                     /region_name="AAA"
                     /note="ATPases associated with a variety of cellular
                     activities; smart00382"
                     /db_xref="CDD:214640"
     CDS             1..446
                     /gene="LOC117607525"
                     /coded_by="XM_034331314.2:217..1557"
                     /db_xref="GeneID:117607525"
ORIGIN      
        1 meehfaalkl ldadndhklt rtisyselps srricieesy ysgnpsslvr qysceqmfgk
       61 ttnsrqfnne newqtigpss nfvepanwqf cksnniihtp kfilpkqsse igcketknmn
      121 mlkdskfslr ysstntnisd erehllcsep kntekaeqkq fnvtndnnrg tyedikkkfr
      181 plaakkkslv kkkgnmccys flclcmfpiv maifaiflnl dsfmicnhtm lfsnaslelq
      241 qkiygqghai snivsflntd tfyvkvlcli ggtgvgksyt aeiiarnfpy kdeifvydri
      301 ledtidtnal nssrsdalli menlkmkdld ffsdamnlls knknkcitvl aifnvkslnn
      361 ylgkeadsvd nenainkvfv dknikflvvp ykplneqile mciteiarns nmsltqsqin
      421 iikqsllffg sgckgayskv qvigre
//
LOCUS       XP_034187214             446 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607525 [Osmia lignaria lignaria].
ACCESSION   XP_034187214
VERSION     XP_034187214.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331323.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187214.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="uncharacterized protein LOC117607525"
                     /calculated_mol_wt=50704
     Region          265..>297
                     /region_name="AAA"
                     /note="ATPases associated with a variety of cellular
                     activities; smart00382"
                     /db_xref="CDD:214640"
     CDS             1..446
                     /gene="LOC117607525"
                     /coded_by="XM_034331323.2:240..1580"
                     /db_xref="GeneID:117607525"
ORIGIN      
        1 meehfaalkl ldadndhklt rtisyselps srricieesy ysgnpsslvr qysceqmfgk
       61 ttnsrqfnne newqtigpss nfvepanwqf cksnniihtp kfilpkqsse igcketknmn
      121 mlkdskfslr ysstntnisd erehllcsep kntekaeqkq fnvtndnnrg tyedikkkfr
      181 plaakkkslv kkkgnmccys flclcmfpiv maifaiflnl dsfmicnhtm lfsnaslelq
      241 qkiygqghai snivsflntd tfyvkvlcli ggtgvgksyt aeiiarnfpy kdeifvydri
      301 ledtidtnal nssrsdalli menlkmkdld ffsdamnlls knknkcitvl aifnvkslnn
      361 ylgkeadsvd nenainkvfv dknikflvvp ykplneqile mciteiarns nmsltqsqin
      421 iikqsllffg sgckgayskv qvigre
//
LOCUS       XP_034187227             400 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607535 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187227
VERSION     XP_034187227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331336.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="uncharacterized protein LOC117607535 isoform X1"
                     /calculated_mol_wt=42993
     CDS             1..400
                     /gene="LOC117607535"
                     /coded_by="XM_034331336.2:828..2030"
                     /db_xref="GeneID:117607535"
ORIGIN      
        1 mlsymlpted kpppgdssqg nfrtnklqks pkersfatrp ppgnvitvsp kkytdtartm
       61 ivskidehgs dqqvqdeqhp mtsespkhng tvvkkstlha skvakddsly sinsdastvg
      121 sdrknkifng akhtslkrvs fgsskgsmve tlvyetpvqe epeinrfldh ngrvpsiitp
      181 vpdtdegrem vrvsllgpqp spttpgvfqv qpiaisriqp idmdpvpael ltthtehtap
      241 ayhtqistds gwdnpfrpdg dlsreadeiv elikggkpit ptpgqtappl pgceavtsna
      301 qttldhdscs spllkssans tnrhansspk pgqengnahs tpmkgatank qqpvnekvap
      361 ssraatveva rmtaqgpgda sqvehvtlkk kpkckccvlq
//
LOCUS       XP_076546234             370 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607535 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546234
VERSION     XP_076546234.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690119.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..370
                     /product="uncharacterized protein LOC117607535 isoform X2"
                     /calculated_mol_wt=40106
     CDS             1..370
                     /gene="LOC117607535"
                     /coded_by="XM_076690119.1:829..1941"
                     /db_xref="GeneID:117607535"
ORIGIN      
        1 mlsymlpted kpppgdssqg nfrtnklqks pkersfatrp ppgnvitvsp kkytdtartm
       61 ivskidehgs dqqvqdeqhp mtsespkhng tvvkkstlha skvakddsly sinsdastvg
      121 sdrknkifng akhtslkrvs fgsskgsmve tlvyetpvqe epeinrfldh ngrvpsiitp
      181 vpdtdegrem vrvsllgpqp spttpgvfqv qpiaisriqp idmdpvpael ltthtehtap
      241 ayhtqistds gwdnpfrpdg dlsreadeiv elikggkpit ptpgqtappl pgceavtsna
      301 qttldhdscs spllkssans tnrhansspk pgqengnahs tpmkgatank qqpvnekvtl
      361 prrreqlqlk
//
LOCUS       XP_034187243             353 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607535 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187243
VERSION     XP_034187243.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331352.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="uncharacterized protein LOC117607535 isoform X3"
                     /calculated_mol_wt=37753
     CDS             1..353
                     /gene="LOC117607535"
                     /coded_by="XM_034331352.2:829..1890"
                     /db_xref="GeneID:117607535"
ORIGIN      
        1 mlsymlpted kpppgdssqg nfrtnklqks pkersfatrp ppgnvitvsp kkytdtartm
       61 ivskidehgs dqqvqdeqhp mtsespkhng tvvkkstlha skvakddsly sinsdastvg
      121 sdrknkifng akhtslkseg remvrvsllg pqpspttpgv fqvqpiaisr iqpidmdpvp
      181 aelltthteh tapayhtqis tdsgwdnpfr pdgdlsread eivelikggk pitptpgqta
      241 pplpgceavt snaqttldhd scsspllkss anstnrhans spkpgqengn ahstpmkgat
      301 ankqqpvnek vapssraatv evarmtaqgp gdasqvehvt lkkkpkckcc vlq
//
LOCUS       XP_034187251             338 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607535 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187251
VERSION     XP_034187251.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331360.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="uncharacterized protein LOC117607535 isoform X4"
                     /calculated_mol_wt=36254
     CDS             1..338
                     /gene="LOC117607535"
                     /coded_by="XM_034331360.2:829..1845"
                     /db_xref="GeneID:117607535"
ORIGIN      
        1 mlsymlpted kpppgdssqg nfrtnklqks pkersfatrp ppgnvitvsp kkytdtartm
       61 ivskidehgs dqqvqdeqhp mtsespkhng tvvkkstlha skvakddsly sinsdastvg
      121 sdrknkifng akhtslkrvs fgsskgsmve tlvyetpvqe epeinrfldh ngrvpsiitp
      181 vpdtdtdsgw dnpfrpdgdl sreadeivel ikggkpitpt pgqtapplpg ceavtsnaqt
      241 tldhdscssp llkssanstn rhansspkpg qengnahstp mkgatankqq pvnekvapss
      301 raatvevarm taqgpgdasq vehvtlkkkp kckccvlq
//
LOCUS       XP_034187265             384 aa            linear   INV 03-SEP-2025
DEFINITION  putative G-protein coupled receptor B0563.6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187265
VERSION     XP_034187265.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331374.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187265.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="putative G-protein coupled receptor B0563.6"
                     /calculated_mol_wt=43310
     Region          32..330
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          32..57
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          63..88
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(85,88..89,107..112,114..115,118,162,164..168,201,
                     204..206,208..210,212..213,276,279..280,282..283,297..298,
                     300..302,305,308..309)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          107..137
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          150..168
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          201..226
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          257..285
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          298..323
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..384
                     /gene="LOC117607551"
                     /coded_by="XM_034331374.2:5832..6986"
                     /db_xref="GeneID:117607551"
ORIGIN      
        1 mfdavnatkt elcfrpedle dprtpslkri sygiilpaic clgivgnifn lvvltrrnmr
       61 gtayiymrgy saaallaisf cipfalrvli hqetgqwnnw lqafyhahle lflgngclgv
      121 gvmmllaltv eryvsvcrpg qhtrplcgpp hltvalipla tfiiylpsaf rgqittclle
      181 pggyvvyqkr enqsyldsmf yqvykvmlem vfkvaptill agfnlrimvv yrkscerrrr
      241 mtlartvsnd edprtfaeer rlvlllgsts ilflvcvspm vilnvtlres nlnnyayqvf
      301 raianllevt nysitfyiyc lfsedfrntl irtlqwpwgk sseggrglpm krsplprati
      361 tttttttppt ttvatpghlt rasv
//
LOCUS       XP_034187277             371 aa            linear   INV 03-SEP-2025
DEFINITION  TM2 and DnaJ domain-containing protein wurst [Osmia lignaria
            lignaria].
ACCESSION   XP_034187277
VERSION     XP_034187277.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331386.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..371
                     /product="TM2 and DnaJ domain-containing protein wurst"
                     /calculated_mol_wt=43027
     Region          25..67
                     /region_name="TM2"
                     /note="TM2 domain; pfam05154"
                     /db_xref="CDD:428337"
     Region          300..359
                     /region_name="DjlA"
                     /note="DnaJ domain-containing protein [Posttranslational
                     modification, protein turnover, chaperones]; COG1076"
                     /db_xref="CDD:440694"
     CDS             1..371
                     /gene="wus"
                     /coded_by="XM_034331386.2:243..1358"
                     /db_xref="GeneID:117607560"
ORIGIN      
        1 melknghvee kekkrtkkqk sifwtyvlwl fgglfgahhv ylerdiqafv yfstfggyfg
       61 lgwlrdiyri psyvrdaned pgflnefkqk vrsngkppfs tvrfaaenav aylwaelfrn
      121 aipedeifgi nfrhlliltp lvialgvwti gnigreqgsi wfalcsaylf yptlsyigdd
      181 tvwlslmiva aslsfdtfsk qwrlkprrkr nlvqrivylg favaiylcli gcylyfnavi
      241 tdsegeeikl seaiqhflts piwldlkasl eatwnqamhq gfwatwtqlv dltdprgein
      301 aykvlglsqt atqtevtarw ralsrdnhpd kikgseeerr qaqekfmeiq qayeilskak
      361 nrrqrrnrks d
//
LOCUS       XP_034187292             336 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-tocopherol transfer protein-like [Osmia lignaria lignaria].
ACCESSION   XP_034187292
VERSION     XP_034187292.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331401.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="alpha-tocopherol transfer protein-like"
                     /calculated_mol_wt=38633
     Region          37..84
                     /region_name="CRAL_TRIO_N"
                     /note="CRAL/TRIO, N-terminal domain; smart01100"
                     /db_xref="CDD:215024"
     Region          106..261
                     /region_name="SEC14"
                     /note="Sec14p-like lipid-binding domain; cd00170"
                     /db_xref="CDD:469559"
     CDS             1..336
                     /gene="LOC117607565"
                     /coded_by="XM_034331401.2:404..1414"
                     /db_xref="GeneID:117607565"
ORIGIN      
        1 mgaiewvtpe dnyecslsee tqklakeelr edkntrdqal eqvrnwikln priqncrlda
       61 qfllrflrck kfnvpmaeea ierylllrqv yhpafnnldm lettieelvs lgylfavpgr
      121 dkkgrrvvva rpgvfdphky tnadmcrmha ityetlmede esqirgfvhf adgagvsfph
      181 ltlftpkeav rivkngerti pmrhkevhai nthpslkfal dfgmslisek ikkrvkiyts
      241 ledainrkmd tsmlpkeygg tmpmkemial wrkellemrp tllshdkmrv cldlysdkar
      301 egavsalkqg fscseigtsd stmygitgsf rklevd
//
LOCUS       XP_034187302             336 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-tocopherol transfer protein-like [Osmia lignaria lignaria].
ACCESSION   XP_034187302
VERSION     XP_034187302.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331411.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="alpha-tocopherol transfer protein-like"
                     /calculated_mol_wt=38633
     Region          37..84
                     /region_name="CRAL_TRIO_N"
                     /note="CRAL/TRIO, N-terminal domain; smart01100"
                     /db_xref="CDD:215024"
     Region          106..261
                     /region_name="SEC14"
                     /note="Sec14p-like lipid-binding domain; cd00170"
                     /db_xref="CDD:469559"
     CDS             1..336
                     /gene="LOC117607565"
                     /coded_by="XM_034331411.2:535..1545"
                     /db_xref="GeneID:117607565"
ORIGIN      
        1 mgaiewvtpe dnyecslsee tqklakeelr edkntrdqal eqvrnwikln priqncrlda
       61 qfllrflrck kfnvpmaeea ierylllrqv yhpafnnldm lettieelvs lgylfavpgr
      121 dkkgrrvvva rpgvfdphky tnadmcrmha ityetlmede esqirgfvhf adgagvsfph
      181 ltlftpkeav rivkngerti pmrhkevhai nthpslkfal dfgmslisek ikkrvkiyts
      241 ledainrkmd tsmlpkeygg tmpmkemial wrkellemrp tllshdkmrv cldlysdkar
      301 egavsalkqg fscseigtsd stmygitgsf rklevd
//
LOCUS       XP_034187313             336 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-tocopherol transfer protein-like [Osmia lignaria lignaria].
ACCESSION   XP_034187313
VERSION     XP_034187313.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331422.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="alpha-tocopherol transfer protein-like"
                     /calculated_mol_wt=38633
     Region          37..84
                     /region_name="CRAL_TRIO_N"
                     /note="CRAL/TRIO, N-terminal domain; smart01100"
                     /db_xref="CDD:215024"
     Region          106..261
                     /region_name="SEC14"
                     /note="Sec14p-like lipid-binding domain; cd00170"
                     /db_xref="CDD:469559"
     CDS             1..336
                     /gene="LOC117607565"
                     /coded_by="XM_034331422.2:383..1393"
                     /db_xref="GeneID:117607565"
ORIGIN      
        1 mgaiewvtpe dnyecslsee tqklakeelr edkntrdqal eqvrnwikln priqncrlda
       61 qfllrflrck kfnvpmaeea ierylllrqv yhpafnnldm lettieelvs lgylfavpgr
      121 dkkgrrvvva rpgvfdphky tnadmcrmha ityetlmede esqirgfvhf adgagvsfph
      181 ltlftpkeav rivkngerti pmrhkevhai nthpslkfal dfgmslisek ikkrvkiyts
      241 ledainrkmd tsmlpkeygg tmpmkemial wrkellemrp tllshdkmrv cldlysdkar
      301 egavsalkqg fscseigtsd stmygitgsf rklevd
//
LOCUS       XP_034187323             244 aa            linear   INV 03-SEP-2025
DEFINITION  twisted gastrulation protein homolog 1-A [Osmia lignaria lignaria].
ACCESSION   XP_034187323
VERSION     XP_034187323.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331432.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..244
                     /product="twisted gastrulation protein homolog 1-A"
                     /calculated_mol_wt=27334
     Region          85..216
                     /region_name="Tsg"
                     /note="Twisted gastrulation (Tsg) protein conserved
                     region; pfam04668"
                     /db_xref="CDD:461385"
     CDS             1..244
                     /gene="LOC117607576"
                     /coded_by="XM_034331432.2:556..1290"
                     /db_xref="GeneID:117607576"
ORIGIN      
        1 mtgwsstlia ivgilvatvi trdseacnea icasvvskcm ltqsckcdlv tctcckecfs
       61 clsylydecc scvdlcpkpn itdnplskks hvedfsepvp glfqaltsep dsherwltft
      121 fpvdfdislf tpkpekemky hiqtteeevh plkpnvmtvn ctvafmaqcn swnkcrascq
      181 smgatsyrwf hdgccecigd tcinygines rcihcpldke eddlkdqydd ygqdeddlte
      241 deid
//
LOCUS       XP_034187332             244 aa            linear   INV 03-SEP-2025
DEFINITION  twisted gastrulation protein homolog 1-A [Osmia lignaria lignaria].
ACCESSION   XP_034187332
VERSION     XP_034187332.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331441.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..244
                     /product="twisted gastrulation protein homolog 1-A"
                     /calculated_mol_wt=27334
     Region          85..216
                     /region_name="Tsg"
                     /note="Twisted gastrulation (Tsg) protein conserved
                     region; pfam04668"
                     /db_xref="CDD:461385"
     CDS             1..244
                     /gene="LOC117607576"
                     /coded_by="XM_034331441.2:201..935"
                     /db_xref="GeneID:117607576"
ORIGIN      
        1 mtgwsstlia ivgilvatvi trdseacnea icasvvskcm ltqsckcdlv tctcckecfs
       61 clsylydecc scvdlcpkpn itdnplskks hvedfsepvp glfqaltsep dsherwltft
      121 fpvdfdislf tpkpekemky hiqtteeevh plkpnvmtvn ctvafmaqcn swnkcrascq
      181 smgatsyrwf hdgccecigd tcinygines rcihcpldke eddlkdqydd ygqdeddlte
      241 deid
//
LOCUS       XP_034187341             244 aa            linear   INV 03-SEP-2025
DEFINITION  twisted gastrulation protein homolog 1-A [Osmia lignaria lignaria].
ACCESSION   XP_034187341
VERSION     XP_034187341.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331450.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..244
                     /product="twisted gastrulation protein homolog 1-A"
                     /calculated_mol_wt=27334
     Region          85..216
                     /region_name="Tsg"
                     /note="Twisted gastrulation (Tsg) protein conserved
                     region; pfam04668"
                     /db_xref="CDD:461385"
     CDS             1..244
                     /gene="LOC117607576"
                     /coded_by="XM_034331450.2:252..986"
                     /db_xref="GeneID:117607576"
ORIGIN      
        1 mtgwsstlia ivgilvatvi trdseacnea icasvvskcm ltqsckcdlv tctcckecfs
       61 clsylydecc scvdlcpkpn itdnplskks hvedfsepvp glfqaltsep dsherwltft
      121 fpvdfdislf tpkpekemky hiqtteeevh plkpnvmtvn ctvafmaqcn swnkcrascq
      181 smgatsyrwf hdgccecigd tcinygines rcihcpldke eddlkdqydd ygqdeddlte
      241 deid
//
LOCUS       XP_034187354             187 aa            linear   INV 03-SEP-2025
DEFINITION  frequenin-2 [Osmia lignaria lignaria].
ACCESSION   XP_034187354
VERSION     XP_034187354.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="frequenin-2"
                     /calculated_mol_wt=21697
     Region          40..173
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..187
                     /gene="LOC117607589"
                     /coded_by="XM_034331463.2:383..946"
                     /db_xref="GeneID:117607589"
ORIGIN      
        1 mgkknsklkq dtidrlttdt yftekeirqw hkgflkdcpd gllteqgfik iykqffpqgd
       61 pskfaslvfr vfdensdgti efeefirals vtsrgnldek lhwafrlydv dndgfitree
      121 mynivdaiye mvgqqphaed entpqkrvdk ifdqmdknhd dkltleefre gskadprivq
      181 alslgge
//
LOCUS       XP_034187361             187 aa            linear   INV 03-SEP-2025
DEFINITION  frequenin-2 [Osmia lignaria lignaria].
ACCESSION   XP_034187361
VERSION     XP_034187361.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331470.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="frequenin-2"
                     /calculated_mol_wt=21697
     Region          40..173
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..187
                     /gene="LOC117607589"
                     /coded_by="XM_034331470.2:394..957"
                     /db_xref="GeneID:117607589"
ORIGIN      
        1 mgkknsklkq dtidrlttdt yftekeirqw hkgflkdcpd gllteqgfik iykqffpqgd
       61 pskfaslvfr vfdensdgti efeefirals vtsrgnldek lhwafrlydv dndgfitree
      121 mynivdaiye mvgqqphaed entpqkrvdk ifdqmdknhd dkltleefre gskadprivq
      181 alslgge
//
LOCUS       XP_034187393             174 aa            linear   INV 03-SEP-2025
DEFINITION  myosin regulatory light chain sqh [Osmia lignaria lignaria].
ACCESSION   XP_034187393
VERSION     XP_034187393.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331502.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..174
                     /product="myosin regulatory light chain sqh"
                     /calculated_mol_wt=19834
     Region          27..164
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..174
                     /gene="sqh"
                     /coded_by="XM_034331502.2:10..534"
                     /db_xref="GeneID:117607604"
ORIGIN      
        1 mssrktagrr attkkraqra tsnvfamfdq aqiaefkeaf nmidqnhdgf idkedlhdml
       61 aslgknptdd ylegmmneap gpinftmflt lfgerlqgtd pedviknafg cfdeentghi
      121 neerlrellt tmgdrftddd vdemyreapi kgsmfdyief trilkhgakd kdeq
//
LOCUS       XP_034196665              58 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612072 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196665
VERSION     XP_034196665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340774.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..58
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..58
                     /product="uncharacterized protein LOC117612072 isoform X1"
                     /calculated_mol_wt=6962
     CDS             1..58
                     /gene="LOC117612072"
                     /coded_by="XM_034340774.2:117..293"
                     /db_xref="GeneID:117612072"
ORIGIN      
        1 mtfirihlma alsirriikr ywaviffptf tavtiyadwn htrlwkkqla eqelqlkn
//
LOCUS       XP_034196675              50 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612072 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196675
VERSION     XP_034196675.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340784.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..50
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..50
                     /product="uncharacterized protein LOC117612072 isoform X2"
                     /calculated_mol_wt=5950
     CDS             1..50
                     /gene="LOC117612072"
                     /coded_by="XM_034340784.2:170..322"
                     /db_xref="GeneID:117612072"
ORIGIN      
        1 maalsirrii krywaviffp tftavtiyad wnhtrlwkkq laeqelqlkn
//
LOCUS       XP_034196682              50 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612072 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196682
VERSION     XP_034196682.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340791.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..50
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..50
                     /product="uncharacterized protein LOC117612072 isoform X2"
                     /calculated_mol_wt=5950
     CDS             1..50
                     /gene="LOC117612072"
                     /coded_by="XM_034340791.2:157..309"
                     /db_xref="GeneID:117612072"
ORIGIN      
        1 maalsirrii krywaviffp tftavtiyad wnhtrlwkkq laeqelqlkn
//
LOCUS       XP_034196691              50 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117612072 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196691
VERSION     XP_034196691.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340800.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..50
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..50
                     /product="uncharacterized protein LOC117612072 isoform X2"
                     /calculated_mol_wt=5950
     CDS             1..50
                     /gene="LOC117612072"
                     /coded_by="XM_034340800.2:107..259"
                     /db_xref="GeneID:117612072"
ORIGIN      
        1 maalsirrii krywaviffp tftavtiyad wnhtrlwkkq laeqelqlkn
//
LOCUS       XP_076546235              56 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546235
VERSION     XP_076546235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690120.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..56
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..56
                     /product="NADH dehydrogenase [ubiquinone] 1 beta
                     subcomplex subunit 1"
                     /calculated_mol_wt=6113
     Region          8..56
                     /region_name="NADH_oxidored"
                     /note="MNLL subunit; pfam08040"
                     /db_xref="CDD:462345"
     CDS             1..56
                     /gene="LOC143305662"
                     /coded_by="XM_076690120.1:1..171"
                     /db_xref="GeneID:143305662"
ORIGIN      
        1 magltkayln lvacmvagys lgwvvnrlet erltsyrdks alfgrelkpg eppswp
//
LOCUS       XP_076546236            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546236
VERSION     XP_076546236.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690121.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_076690121.1:1301..11062"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_076546237            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546237
VERSION     XP_076546237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690122.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_076690122.1:1301..11062"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_034176777            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176777
VERSION     XP_034176777.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320886.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_034320886.2:1305..11066"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_034176786            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176786
VERSION     XP_034176786.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320895.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_034320895.2:1531..11292"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_076546238            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546238
VERSION     XP_076546238.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690123.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_076690123.1:1301..11062"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_034176824            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176824
VERSION     XP_034176824.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320933.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_034320933.2:1261..11022"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_034176768            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176768
VERSION     XP_034176768.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320877.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_034320877.2:1301..11062"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_076546239            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546239
VERSION     XP_076546239.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690124.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_076690124.1:1170..10931"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_034176844            3253 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176844
VERSION     XP_034176844.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320953.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3253
                     /product="microtubule binding protein furry isoform X1"
                     /calculated_mol_wt=360752
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3220
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3253
                     /gene="fry"
                     /coded_by="XM_034320953.2:68..9829"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstrsd
     2881 tsgssagdlg dvtpcnaspn lsalmpfrqv vrddaeeiwr qqiqnlitqs lyntldffhl
     2941 lsrilrdvss ksvtitreas allcngspaa tqlgshlssh aellsskaep pliwfsiaif
     3001 anqrlneslr fgmleiqehl etfldkkdha tecleaakaa aklqalgggh tteagleeml
     3061 ldlgkalykl hlqlllliea snkmlgtlmn aaknvqiplq disieianmk ialsralees
     3121 tesergtptp tpspsplpgt gaveevarva ellrnarwcp aleavrlhra qwpgdpfhdd
     3181 ddvttavnmy ckylgqdrsd ifvvtrksde mseifsrlme slfqvlgavt gletstkaar
     3241 sqhdhrsnsk ndc
//
LOCUS       XP_034176861            3252 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176861
VERSION     XP_034176861.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320970.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3252
                     /product="microtubule binding protein furry isoform X2"
                     /calculated_mol_wt=360623
     Region          238..770
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1092..1879
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2344..2596
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2630..3219
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3252
                     /gene="fry"
                     /coded_by="XM_034320970.2:1301..11059"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpas epralhgegl hetaaesssq svpepnqdll
       61 tvhgatqges sdavsihtas tsvsgnesss srvsaitvvl pwgaqketvk pqqmgdmdlr
      121 pgefvmrtlf aeftsqaekk meavmtehek plskvlqrge dlqfdqllsa fgsvaehclp
      181 silralfnwy erqlvdqgsd qkkpapgked qkgksimyti vsgsvetver seadllqerr
      241 dlavefifcl mlievlrqlp fhpghedlvt yieniafkhf kyregiqnep naanihiiad
      301 lyaevigvla qarfmsvrkr fmvelkelra kepgphttqs iisllmgmkf frvkmvpiee
      361 feasfqfmqe caqyflevkd kdvkhalagl fveilvpvaa avknevnvpc lknfvemlys
      421 ttldmctksk hrlalfplvt cllcvsqktf flqnwhyfla mclshlknrd pkmcrvalea
      481 lyrllwvymi rikcesnsat qsrlqsivns lfpkgskavv prdtplnifv kiiqfiaqer
      541 ldfamreivf dllsvgrpvk iiltpermsi glraflvvad slqqkegepp mprtmgvlps
      601 gntmrvkktf lnkmltedta rsigmssyfp hvrrvfvdil raldvhygrp lmmtstqnmn
      661 kepdemitge rkpridlfrt cvaavprlip dgmtgaelvd llsrltvhmd eelrglayqs
      721 lqtlvldfpd wrqdvvlgft qflardvqdt fpqlvdnglr mllqlltswk nsltspsirs
      781 keqsdntrtn trtntdgnik ksesgqkvep vssvfhlveg falvmlcncr lyprriavhi
      841 lrevklllka lggveddqav idvidaccpv vlekcyymlp paekaaaast snvdlqwiae
      901 rsscvwtagl hedtstksss slnlngadpw gsclfaflek drvltlcpta iahswpivft
      961 rinslfsvid ptpvndnras llrsstavrk pvnerdmymh vwknyltfgy rvvppvpspv
     1021 vrcaspdlsl sgqhtvefgv lcmskelsqs lenlggaqtl pgrfsvpsis giytrhkrts
     1081 sspdslsaer gdnkspgtna spaalykltv pllrcevvdv rdaavqaigk vnadalkdlm
     1141 eelvpyirea vdrkqenmrr rrrrdalrlq lvrvleliae ygtfgicpav ldretqslhp
     1201 tfveyidgar lylenetdke apavrdiklh fcnfmrkmik sfsletchtl lkrdlrrnlf
     1261 mlfaswagpy grplastssl heeeksctel qlsalqamsg llccgpcfns qslseegail
     1321 yqwldlllas kdekiyalar etvvlllecn pdigtlldwv vdrcytgapq vadgcflala
     1381 tifsareypc dhytsiinvt lmstgcprap vhdaalqllq lldqrffgnv gplpatepet
     1441 sqddpvggss ttatsaagpq snpiggmsss gtirggtldv llsttycrnq mylsrqlaql
     1501 hpeltmpmfs eithrfqtar revrqlllqy llpwlhnmel vdpnvpppsn plsyyqyyas
     1561 dmsrggarre gwgsaeatem vlnnlfyita kfsdehpket eelwatlcgc wpnnlkviir
     1621 yliivsgmap qellpyakrv vlylararpd rlvdemmtel qtvetlncli ertetppfyr
     1681 ltsmrkassh sdapaadpgn pprdlgvekg tihtkrhsge dpvktgsksd talralagfq
     1741 tpraektrta sgppvlpddl stptteaeli tdescyggrn gpvgvngkms csgekfdipq
     1801 phplpmpeyg gyfaplteyl pdssqpisgf hrcniavmll tdvvvdgiql dwaihvplml
     1861 hivflgldhs rplvrdhcrc lllnllvvlg dhrdhlgvar vllaskteql glglstpalp
     1921 ilehnftevd pefdsylygp ppapppttpp psssppppss sppppppppp pppppppppe
     1981 ssifnaappp pppppppppl pssntgtpth spipnststp pmsmnhvnqs vmdsnkdysn
     2041 shaydsgtcn nwpqttgsst tvppvivtpe dannwvgpqp gpnmpiqdvi kslinflasr
     2101 inqplwnyed mtakvwwvrs aeqltvllrh vlrvfrdslp halvsqrwaq talqlglscs
     2161 srhyagrslq vfralrvpit srmlsdilsr lvetvaeqge dmqgyvtell ltleaavdsl
     2221 esdfrpldfm keifkstpnl nnkdpasgcl iggkrspggg ngyptgphmf shlsqaghtr
     2281 stsysvsycm kkssagnsta seikeldtrc nkypnsnlsr srsaqslkll gdsatqddkm
     2341 tilaqlfwls vsllesdyeh efllalrlls rvlhrlpldr pdardkvekl qqqlrwnsfp
     2401 gvhalllkgc tspntyeqvv tllsqftpll dlpvvdptqs lafpmnvvsl lpymllnyed
     2461 anelcirsae niaqvsaekg kklenlgtvm tlysrrtfsk esfqwtkcvv kylydtyahl
     2521 sfnmlaflve vlekgpgsva lpvlsiihcm lhyvdlasqa aqpintellr viskyvegph
     2581 wkealkilkl vvtrsstlva pptsvhgssw essiasphps ftdteiftkk elpgrtmdft
     2641 fdlsqtpvig rrwlirqgve ekslasprcs lslspadsna vsgwkrpwms qgrvreclvn
     2701 llttcgqrvg lpkspsvifs qssdlmerqs smassteevs ganndlsggs rrddeqfgvf
     2761 kdfdfleyes esvegestdn fnwgvrrrpl segeerepsl rqfeeslsek tqssskhtsr
     2821 rgvaeessdd eagsesplde vpggsgagqe annipgmfpp sslslipsrt rhdsstrsdt
     2881 sgssagdlgd vtpcnaspnl salmpfrqvv rddaeeiwrq qiqnlitqsl yntldffhll
     2941 srilrdvssk svtitreasa llcngspaat qlgshlssha ellsskaepp liwfsiaifa
     3001 nqrlneslrf gmleiqehle tfldkkdhat ecleaakaaa klqalggght teagleemll
     3061 dlgkalyklh lqllllieas nkmlgtlmna aknvqiplqd isieianmki alsraleest
     3121 esergtptpt pspsplpgtg aveevarvae llrnarwcpa leavrlhraq wpgdpfhddd
     3181 dvttavnmyc kylgqdrsdi fvvtrksdem seifsrlmes lfqvlgavtg letstkaars
     3241 qhdhrsnskn dc
//
LOCUS       XP_034176869            3249 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176869
VERSION     XP_034176869.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320978.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3249
                     /product="microtubule binding protein furry isoform X3"
                     /calculated_mol_wt=360292
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2345..2597
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2631..3216
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3249
                     /gene="fry"
                     /coded_by="XM_034320978.2:1301..11050"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaydsgtc nnwpqttgss ttvppvivtp edannwvgpq pgpnmpiqdv ikslinflas
     2101 rinqplwnye dmtakvwwvr saeqltvllr hvlrvfrdsl phalvsqrwa qtalqlglsc
     2161 ssrhyagrsl qvfralrvpi tsrmlsdils rlvetvaeqg edmqgyvtel lltleaavds
     2221 lesdfrpldf mkeifkstpn lnnkdpasgc liggkrspgg gngyptgphm fshlsqaght
     2281 rstsysvsyc mkkssagnst aseikeldtr cnkypnsnls rsrsaqslkl lgdsatqddk
     2341 mtilaqlfwl svsllesdye hefllalrll srvlhrlpld rpdardkvek lqqqlrwnsf
     2401 pgvhalllkg ctspntyeqv vtllsqftpl ldlpvvdptq slafpmnvvs llpymllnye
     2461 danelcirsa eniaqvsaek gkklenlgtv mtlysrrtfs kesfqwtkcv vkylydtyah
     2521 lsfnmlaflv evlekgpgsv alpvlsiihc mlhyvdlasq aaqpintell rviskyvegp
     2581 hwkealkilk lvvtrsstlv apptsvhgss wessiasphp sftdteiftk kelpgrtmdf
     2641 tfdlsqtpvi grrwlirqgv eekslasprc slslspadsn avsgwkrpwm sqgrvreclv
     2701 nllttcgqrv glpkspsvif sqssdlmerq ssmassteev sganndlsgg srrddeqfgv
     2761 fkdfdfleye sesvegestd nfnwgvrrrp lsegeereps lrqfeeslse ktqssskhts
     2821 rrgvaeessd deagsespld evpggsgagq eannipgmfp psslslipsr trhdsstsgs
     2881 sagdlgdvtp cnaspnlsal mpfrqvvrdd aeeiwrqqiq nlitqslynt ldffhllsri
     2941 lrdvssksvt itreasallc ngspaatqlg shlsshaell sskaeppliw fsiaifanqr
     3001 lneslrfgml eiqehletfl dkkdhatecl eaakaaaklq alggghttea gleemlldlg
     3061 kalyklhlql lllieasnkm lgtlmnaakn vqiplqdisi eianmkials raleestese
     3121 rgtptptpsp splpgtgave evarvaellr narwcpalea vrlhraqwpg dpfhddddvt
     3181 tavnmyckyl gqdrsdifvv trksdemsei fsrlmeslfq vlgavtglet stkaarsqhd
     3241 hrsnskndc
//
LOCUS       XP_034176877            3241 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176877
VERSION     XP_034176877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3241
                     /product="microtubule binding protein furry isoform X4"
                     /calculated_mol_wt=359446
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1093..1880
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2333..2585
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2619..3208
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3241
                     /gene="fry"
                     /coded_by="XM_034320986.2:1301..11026"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lsgqhtvefg vlcmskelsq slenlggaqt lpgrfsvpsi sgiytrhkrt
     1081 ssspdslsae rgdnkspgtn aspaalyklt vpllrcevvd vrdaavqaig kvnadalkdl
     1141 meelvpyire avdrkqenmr rrrrrdalrl qlvrvlelia eygtfgicpa vldretqslh
     1201 ptfveyidga rlylenetdk eapavrdikl hfcnfmrkmi ksfsletcht llkrdlrrnl
     1261 fmlfaswagp ygrplastss lheeekscte lqlsalqams gllccgpcfn sqslseegai
     1321 lyqwldllla skdekiyala retvvlllec npdigtlldw vvdrcytgap qvadgcflal
     1381 atifsareyp cdhytsiinv tlmstgcpra pvhdaalqll qlldqrffgn vgplpatepe
     1441 tsqddpvggs sttatsaagp qsnpiggmss sgtirggtld vllsttycrn qmylsrqlaq
     1501 lhpeltmpmf seithrfqta rrevrqlllq yllpwlhnme lvdpnvppps nplsyyqyya
     1561 sdmsrggarr egwgsaeate mvlnnlfyit akfsdehpke teelwatlcg cwpnnlkvii
     1621 ryliivsgma pqellpyakr vvlylararp drlvdemmte lqtvetlncl iertetppfy
     1681 rltsmrkass hsdapaadpg npprdlgvek gtihtkrhsg edpvktgsks dtalralagf
     1741 qtpraektrt asgppvlpdd lstptteael itdescyggr ngpvgvngkm scsgekfdip
     1801 qphplpmpey ggyfapltey lpdssqpisg fhrcniavml ltdvvvdgiq ldwaihvplm
     1861 lhivflgldh srplvrdhcr clllnllvvl gdhrdhlgva rvllaskteq lglglstpal
     1921 pilehnftev dpefdsylyg pppapppttp ppssspppps sspppppppp pppppppppp
     1981 essifnaapp pppppppppp lpssntgtpt hspipnstst ppmsmnhvnq svmdsnkdys
     2041 nshaygsstt vppvivtped annwvgpqpg pnmpiqdvik slinflasri nqplwnyedm
     2101 takvwwvrsa eqltvllrhv lrvfrdslph alvsqrwaqt alqlglscss rhyagrslqv
     2161 fralrvpits rmlsdilsrl vetvaeqged mqgyvtelll tleaavdsle sdfrpldfmk
     2221 eifkstpnln nkdpasgcli ggkrspgggn gyptgphmfs hlsqaghtrs tsysvsycmk
     2281 kssagnstas eikeldtrcn kypnsnlsrs rsaqslkllg dsatqddkmt ilaqlfwlsv
     2341 sllesdyehe fllalrllsr vlhrlpldrp dardkveklq qqlrwnsfpg vhalllkgct
     2401 spntyeqvvt llsqftplld lpvvdptqsl afpmnvvsll pymllnyeda nelcirsaen
     2461 iaqvsaekgk klenlgtvmt lysrrtfske sfqwtkcvvk ylydtyahls fnmlaflvev
     2521 lekgpgsval pvlsiihcml hyvdlasqaa qpintellrv iskyvegphw kealkilklv
     2581 vtrsstlvap ptsvhgsswe ssiasphpsf tdteiftkke lpgrtmdftf dlsqtpvigr
     2641 rwlirqgvee kslasprcsl slspadsnav sgwkrpwmsq grvreclvnl lttcgqrvgl
     2701 pkspsvifsq ssdlmerqss massteevsg anndlsggsr rddeqfgvfk dfdfleyese
     2761 svegestdnf nwgvrrrpls egeerepslr qfeeslsekt qssskhtsrr gvaeessdde
     2821 agsespldev pggsgagqea nnipgmfpps slslipsrtr hdsstrsdts gssagdlgdv
     2881 tpcnaspnls almpfrqvvr ddaeeiwrqq iqnlitqsly ntldffhlls rilrdvssks
     2941 vtitreasal lcngspaatq lgshlsshae llsskaeppl iwfsiaifan qrlneslrfg
     3001 mleiqehlet fldkkdhate cleaakaaak lqalggghtt eagleemlld lgkalyklhl
     3061 qllllieasn kmlgtlmnaa knvqiplqdi sieianmkia lsraleeste sergtptptp
     3121 spsplpgtga veevarvael lrnarwcpal eavrlhraqw pgdpfhdddd vttavnmyck
     3181 ylgqdrsdif vvtrksdems eifsrlmesl fqvlgavtgl etstkaarsq hdhrsnsknd
     3241 c
//
LOCUS       XP_076546240            3204 aa            linear   INV 03-SEP-2025
DEFINITION  microtubule binding protein furry isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546240
VERSION     XP_076546240.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690125.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3204
                     /product="microtubule binding protein furry isoform X5"
                     /calculated_mol_wt=355464
     Region          239..771
                     /region_name="MOR2-PAG1_N"
                     /note="Cell morphogenesis N-terminal; pfam14222"
                     /db_xref="CDD:464107"
     Region          1044..1831
                     /region_name="MOR2-PAG1_mid"
                     /note="Cell morphogenesis central region; pfam14228"
                     /db_xref="CDD:433790"
     Region          2296..2548
                     /region_name="MOR2-PAG1_C"
                     /note="Cell morphogenesis C-terminal; pfam14225"
                     /db_xref="CDD:464109"
     Region          2582..3171
                     /region_name="Fry_C"
                     /note="Furry protein C-terminal; pfam19421"
                     /db_xref="CDD:466072"
     CDS             1..3204
                     /gene="fry"
                     /coded_by="XM_076690125.1:1301..10915"
                     /db_xref="GeneID:117602638"
ORIGIN      
        1 mlpedkayid nmteggetqp tpgneaqpea sepralhgeg lhetaaesss qsvpepnqdl
       61 ltvhgatqge ssdavsihta stsvsgness ssrvsaitvv lpwgaqketv kpqqmgdmdl
      121 rpgefvmrtl faeftsqaek kmeavmtehe kplskvlqrg edlqfdqlls afgsvaehcl
      181 psilralfnw yerqlvdqgs dqkkpapgke dqkgksimyt ivsgsvetve rseadllqer
      241 rdlavefifc lmlievlrql pfhpghedlv tyieniafkh fkyregiqne pnaanihiia
      301 dlyaevigvl aqarfmsvrk rfmvelkelr akepgphttq siisllmgmk ffrvkmvpie
      361 efeasfqfmq ecaqyflevk dkdvkhalag lfveilvpva aavknevnvp clknfvemly
      421 sttldmctks khrlalfplv tcllcvsqkt fflqnwhyfl amclshlknr dpkmcrvale
      481 alyrllwvym irikcesnsa tqsrlqsivn slfpkgskav vprdtplnif vkiiqfiaqe
      541 rldfamreiv fdllsvgrpv kiiltperms iglraflvva dslqqkegep pmprtmgvlp
      601 sgntmrvkkt flnkmltedt arsigmssyf phvrrvfvdi lraldvhygr plmmtstqnm
      661 nkepdemitg erkpridlfr tcvaavprli pdgmtgaelv dllsrltvhm deelrglayq
      721 slqtlvldfp dwrqdvvlgf tqflardvqd tfpqlvdngl rmllqlltsw knsltspsir
      781 skeqsdntrt ntrtntdgni kksesgqkve pvssvfhlve gfalvmlcnc rlyprriavh
      841 ilrevklllk alggveddqa vidvidaccp vvlekcyyml ppaekaaaas tsnvdlqwia
      901 ersscvwtag lhedtstkss sslnlngadp wgsclfafle kdrvltlcpt aiahswpivf
      961 trinslfsvi dptpvndnra sllrsstavr kpvnerdmym hvwknyltfg yrvvppvpsp
     1021 vvrcaspdls lssspdslsa ergdnkspgt naspaalykl tvpllrcevv dvrdaavqai
     1081 gkvnadalkd lmeelvpyir eavdrkqenm rrrrrrdalr lqlvrvleli aeygtfgicp
     1141 avldretqsl hptfveyidg arlylenetd keapavrdik lhfcnfmrkm iksfsletch
     1201 tllkrdlrrn lfmlfaswag pygrplasts slheeeksct elqlsalqam sgllccgpcf
     1261 nsqslseega ilyqwldlll askdekiyal aretvvllle cnpdigtlld wvvdrcytga
     1321 pqvadgcfla latifsarey pcdhytsiin vtlmstgcpr apvhdaalql lqlldqrffg
     1381 nvgplpatep etsqddpvgg ssttatsaag pqsnpiggms ssgtirggtl dvllsttycr
     1441 nqmylsrqla qlhpeltmpm fseithrfqt arrevrqlll qyllpwlhnm elvdpnvppp
     1501 snplsyyqyy asdmsrggar regwgsaeat emvlnnlfyi takfsdehpk eteelwatlc
     1561 gcwpnnlkvi iryliivsgm apqellpyak rvvlylarar pdrlvdemmt elqtvetlnc
     1621 liertetppf yrltsmrkas shsdapaadp gnpprdlgve kgtihtkrhs gedpvktgsk
     1681 sdtalralag fqtpraektr tasgppvlpd dlstptteae litdescygg rngpvgvngk
     1741 mscsgekfdi pqphplpmpe yggyfaplte ylpdssqpis gfhrcniavm lltdvvvdgi
     1801 qldwaihvpl mlhivflgld hsrplvrdhc rclllnllvv lgdhrdhlgv arvllaskte
     1861 qlglglstpa lpilehnfte vdpefdsyly gpppappptt pppssspppp sssppppppp
     1921 pppppppppp pessifnaap pppppppppp plpssntgtp thspipnsts tppmsmnhvn
     1981 qsvmdsnkdy snshaydsgt cnnwpqttgs sttvppvivt pedannwvgp qpgpnmpiqd
     2041 vikslinfla srinqplwny edmtakvwwv rsaeqltvll rhvlrvfrds lphalvsqrw
     2101 aqtalqlgls cssrhyagrs lqvfralrvp itsrmlsdil srlvetvaeq gedmqgyvte
     2161 llltleaavd slesdfrpld fmkeifkstp nlnnkdpasg cliggkrspg ggngyptgph
     2221 mfshlsqagh trstsysvsy cmkkssagns taseikeldt rcnkypnsnl srsrsaqslk
     2281 llgdsatqdd kmtilaqlfw lsvsllesdy ehefllalrl lsrvlhrlpl drpdardkve
     2341 klqqqlrwns fpgvhalllk gctspntyeq vvtllsqftp lldlpvvdpt qslafpmnvv
     2401 sllpymllny edanelcirs aeniaqvsae kgkklenlgt vmtlysrrtf skesfqwtkc
     2461 vvkylydtya hlsfnmlafl vevlekgpgs valpvlsiih cmlhyvdlas qaaqpintel
     2521 lrviskyveg phwkealkil klvvtrsstl vapptsvhgs swessiasph psftdteift
     2581 kkelpgrtmd ftfdlsqtpv igrrwlirqg veekslaspr cslslspads navsgwkrpw
     2641 msqgrvrecl vnllttcgqr vglpkspsvi fsqssdlmer qssmasstee vsganndlsg
     2701 gsrrddeqfg vfkdfdfley esesvegest dnfnwgvrrr plsegeerep slrqfeesls
     2761 ektqssskht srrgvaeess ddeagsespl devpggsgag qeannipgmf ppsslslips
     2821 rtrhdsstrs dtsgssagdl gdvtpcnasp nlsalmpfrq vvrddaeeiw rqqiqnlitq
     2881 slyntldffh llsrilrdvs sksvtitrea sallcngspa atqlgshlss haellsskae
     2941 ppliwfsiai fanqrlnesl rfgmleiqeh letfldkkdh atecleaaka aaklqalggg
     3001 htteagleem lldlgkalyk lhlqllllie asnkmlgtlm naaknvqipl qdisieianm
     3061 kialsralee stesergtpt ptpspsplpg tgaveevarv aellrnarwc paleavrlhr
     3121 aqwpgdpfhd dddvttavnm yckylgqdrs difvvtrksd emseifsrlm eslfqvlgav
     3181 tgletstkaa rsqhdhrsns kndc
//
LOCUS       XP_034176898            2402 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176898
VERSION     XP_034176898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321007.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2402
                     /product="CCR4-NOT transcription complex subunit 1 isoform
                     X1"
                     /calculated_mol_wt=269371
     Region          506..660
                     /region_name="CNOT1_HEAT"
                     /note="CCR4-NOT transcription complex subunit 1 HEAT
                     repeat; pfam16418"
                     /db_xref="CDD:465113"
     Region          <836..2387
                     /region_name="CDC39"
                     /note="Cell division control protein, negative regulator
                     of transcription [Cell division and chromosome
                     partitioning / Transcription]; COG5103"
                     /db_xref="CDD:227434"
     CDS             1..2402
                     /gene="Not1"
                     /coded_by="XM_034321007.2:411..7619"
                     /db_xref="GeneID:117602693"
ORIGIN      
        1 mnldslsftl sqisylvanl nkknfrdscq eisvlvqwkg leadrhllrs llscidfstg
       61 eppessakdy fqvqllkqec tnllskpsfi snlcfaidhp fhqqktlnps pkfflrlkkv
      121 lgltlvqeva faiallhsen teirtlaleh vkkqlpelik nyinsetsnk hqeeglhdsl
      181 pevlhlilsq afhsanqfgl sseakekflk nlrrdfprel vpvvlaplly pdngetsqak
      241 ielnmavnqm dgnslvelim elgygftssv decrsalagl gareispaca arvlahmars
      301 ynslddaggl qsfwgnsgat qdankeksad svapttwnve vfiqalkeiq stlswneviv
      361 kldhaefvvk drqglsllit glrmglqhqg yppdmfpvel fyrhwdnveg qfslvqqilk
      421 cpdifcfady pyhsvtvdvl kaapesdske aqtwrslnvv elllhmaerg lygpvqeifk
      481 wpiqhcpdvl vlallqinap vtilrqells tlmpiflgnh pnsavilhha whgntvkiks
      541 iimhamaewy irgdhdqtrl srildvaqdl kalthlltaq sypfvidlac lasrreylkl
      601 ekwltdkire qgevfvaacv kflqrrcpqv mgpgikedit vpkasqlpqe tlttmlaclq
      661 vcagsvsqec sdaimtmvqn cslmlskstm srppppgvlr hrglepfnpa tlggqlfssk
      721 qvdplgnlss slasmglgss lgppsssafn lpgalgplvs apgspsrlmg pspspfpmlp
      781 lssqlhsgpv gtqgpsvlpg sttigglgrl gpptgiekar ipetsnlfpd msqnvskeie
      841 deansyfqri ynhpphptls idevldmlkk fqdsgskrer evfncmlrnl feeyrffpqy
      901 pdkelqitaq lfggiiergl vnsymtlgla lrfvldalrk pegskmyyfg italdrfksr
      961 lkdyqtyceh vraiqhfsef pphlieyiey glqgqepptr pqgpvlpktl aamlapvttp
     1021 yktittttit tsttqaksst tpttslsarp sianatnidt llvatdkeek itsppealqd
     1081 ktafifnnls qlnlqqkcde ireivteeyw pwmaqylvmk rasielnfha lysnfldclk
     1141 lpevnkmvtr etfrnikvll rsdkgianfs drsllknlgh wlgmltlgrn kpilqididl
     1201 ksllveayhk gqqellyvvp fvakvlesca ksrvfrppnp wtmaimnvla elhqepdlkl
     1261 nlkfeievlc knlsidvgel kpalylkdpe klrnleyqls hpnkksesnn nqqqtqgpie
     1321 elvgstttgt ivpqtapant tpslptgppe prfnymdisv tgianisqhi tinnqlplfq
     1381 thphlkqfvr paveraiqew ihpvvdrsik ialttseqiv rkdfaldpee vrmrtaarhm
     1441 vrnltagmam itcrdqvmas istnlkqafl tammgttpqq kelaeqaanv vaadnmelac
     1501 afvqktaiek aipemdkrll neielrkiar qegrrycdpl akyqaermpe qirlkvggvt
     1561 aqqmavyeef arnipgflpl serdtqallm pkpvteptvt afaanpavav ataqqvaaya
     1621 aavsndevga mleklaaeve vllaamgpav pppqhaalhs llesiiltrr srdagaamtl
     1681 lkkaveglld gpsissgvid seiilryrel hlrilkclqd praygmqwtn khvtrcltec
     1741 reefrynfea vdylirshli slpqydlala qaidsgnama tafamqlvql yliderqtth
     1801 vtesdlfhti eilarmahhr appegillfr ltslieslra nhdpgvladr apagptahih
     1861 sgilqvrard fddppglmek teyllrewvs mhhspthard ptkafgmfvh qmnihgilkt
     1921 ddlitrffkl stqmcvdlcy ralsetsaap svvrakcfhs ldafvrlval lvkhsgdstn
     1981 thtkinllnk vlgivagvll qdheirgtdf qqlpyhrifi mlflelcape pvleavnyqv
     2041 ltafchtlhi lrpakasgfc yawlelvshr vfigrmlait pqqkcwgmya qllidlfkyl
     2101 apylrnaela kpvtllykgt lrvllvllhd fpeflcdyhy gfcdvippnc iqmrnlilsa
     2161 fprnmrlpdp ftpnlkvdml qeiahaprvl tnfasmiqpl sfkkeldsyl karapvtfls
     2221 elrsnlqvsq eagvryniql mnalvlyvgt qaiayirskg htpnmstiah sahmdifqnl
     2281 avdldtegry lflnaianql rypnshthyf sctllylfae anteaiqeqi trvllerliv
     2341 nrphpwglli tfieliknpt ykfwthefvh capeieklfe svarscmvqk qvqptpepdi
     2401 pe
//
LOCUS       XP_034176907            2401 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176907
VERSION     XP_034176907.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321016.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2401
                     /product="CCR4-NOT transcription complex subunit 1 isoform
                     X2"
                     /calculated_mol_wt=269284
     Region          506..660
                     /region_name="CNOT1_HEAT"
                     /note="CCR4-NOT transcription complex subunit 1 HEAT
                     repeat; pfam16418"
                     /db_xref="CDD:465113"
     Region          <835..2386
                     /region_name="CDC39"
                     /note="Cell division control protein, negative regulator
                     of transcription [Cell division and chromosome
                     partitioning / Transcription]; COG5103"
                     /db_xref="CDD:227434"
     CDS             1..2401
                     /gene="Not1"
                     /coded_by="XM_034321016.2:412..7617"
                     /db_xref="GeneID:117602693"
ORIGIN      
        1 mnldslsftl sqisylvanl nkknfrdscq eisvlvqwkg leadrhllrs llscidfstg
       61 eppessakdy fqvqllkqec tnllskpsfi snlcfaidhp fhqqktlnps pkfflrlkkv
      121 lgltlvqeva faiallhsen teirtlaleh vkkqlpelik nyinsetsnk hqeeglhdsl
      181 pevlhlilsq afhsanqfgl sseakekflk nlrrdfprel vpvvlaplly pdngetsqak
      241 ielnmavnqm dgnslvelim elgygftssv decrsalagl gareispaca arvlahmars
      301 ynslddaggl qsfwgnsgat qdankeksad svapttwnve vfiqalkeiq stlswneviv
      361 kldhaefvvk drqglsllit glrmglqhqg yppdmfpvel fyrhwdnveg qfslvqqilk
      421 cpdifcfady pyhsvtvdvl kaapesdske aqtwrslnvv elllhmaerg lygpvqeifk
      481 wpiqhcpdvl vlallqinap vtilrqells tlmpiflgnh pnsavilhha whgntvkiks
      541 iimhamaewy irgdhdqtrl srildvaqdl kalthlltaq sypfvidlac lasrreylkl
      601 ekwltdkire qgevfvaacv kflqrrcpqv mgpgikedit vpkasqlpqe tlttmlaclq
      661 vcagvsqecs daimtmvqnc slmlskstms rppppgvlrh rglepfnpat lggqlfsskq
      721 vdplgnlsss lasmglgssl gppsssafnl pgalgplvsa pgspsrlmgp spspfpmlpl
      781 ssqlhsgpvg tqgpsvlpgs ttigglgrlg pptgiekari petsnlfpdm sqnvskeied
      841 eansyfqriy nhpphptlsi devldmlkkf qdsgskrere vfncmlrnlf eeyrffpqyp
      901 dkelqitaql fggiierglv nsymtlglal rfvldalrkp egskmyyfgi taldrfksrl
      961 kdyqtycehv raiqhfsefp phlieyieyg lqgqepptrp qgpvlpktla amlapvttpy
     1021 ktittttitt sttqaksstt pttslsarps ianatnidtl lvatdkeeki tsppealqdk
     1081 tafifnnlsq lnlqqkcdei reivteeywp wmaqylvmkr asielnfhal ysnfldclkl
     1141 pevnkmvtre tfrnikvllr sdkgianfsd rsllknlghw lgmltlgrnk pilqididlk
     1201 sllveayhkg qqellyvvpf vakvlescak srvfrppnpw tmaimnvlae lhqepdlkln
     1261 lkfeievlck nlsidvgelk palylkdpek lrnleyqlsh pnkksesnnn qqqtqgpiee
     1321 lvgstttgti vpqtapantt pslptgppep rfnymdisvt gianisqhit innqlplfqt
     1381 hphlkqfvrp averaiqewi hpvvdrsiki alttseqivr kdfaldpeev rmrtaarhmv
     1441 rnltagmami tcrdqvmasi stnlkqaflt ammgttpqqk elaeqaanvv aadnmelaca
     1501 fvqktaieka ipemdkrlln eielrkiarq egrrycdpla kyqaermpeq irlkvggvta
     1561 qqmavyeefa rnipgflpls erdtqallmp kpvteptvta faanpavava taqqvaayaa
     1621 avsndevgam leklaaevev llaamgpavp ppqhaalhsl lesiiltrrs rdagaamtll
     1681 kkaveglldg psissgvids eiilryrelh lrilkclqdp raygmqwtnk hvtrcltecr
     1741 eefrynfeav dylirshlis lpqydlalaq aidsgnamat afamqlvqly liderqtthv
     1801 tesdlfhtie ilarmahhra ppegillfrl tslieslran hdpgvladra pagptahihs
     1861 gilqvrardf ddppglmekt eyllrewvsm hhspthardp tkafgmfvhq mnihgilktd
     1921 dlitrffkls tqmcvdlcyr alsetsaaps vvrakcfhsl dafvrlvall vkhsgdstnt
     1981 htkinllnkv lgivagvllq dheirgtdfq qlpyhrifim lflelcapep vleavnyqvl
     2041 tafchtlhil rpakasgfcy awlelvshrv figrmlaitp qqkcwgmyaq llidlfkyla
     2101 pylrnaelak pvtllykgtl rvllvllhdf peflcdyhyg fcdvippnci qmrnlilsaf
     2161 prnmrlpdpf tpnlkvdmlq eiahaprvlt nfasmiqpls fkkeldsylk arapvtflse
     2221 lrsnlqvsqe agvryniqlm nalvlyvgtq aiayirskgh tpnmstiahs ahmdifqnla
     2281 vdldtegryl flnaianqlr ypnshthyfs ctllylfaea nteaiqeqit rvllerlivn
     2341 rphpwgllit fieliknpty kfwthefvhc apeieklfes varscmvqkq vqptpepdip
     2401 e
//
LOCUS       XP_034176916            2400 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176916
VERSION     XP_034176916.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321025.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2400
                     /product="CCR4-NOT transcription complex subunit 1 isoform
                     X3"
                     /calculated_mol_wt=269115
     Region          506..660
                     /region_name="CNOT1_HEAT"
                     /note="CCR4-NOT transcription complex subunit 1 HEAT
                     repeat; pfam16418"
                     /db_xref="CDD:465113"
     Region          <836..2385
                     /region_name="CDC39"
                     /note="Cell division control protein, negative regulator
                     of transcription [Cell division and chromosome
                     partitioning / Transcription]; COG5103"
                     /db_xref="CDD:227434"
     CDS             1..2400
                     /gene="Not1"
                     /coded_by="XM_034321025.2:412..7614"
                     /db_xref="GeneID:117602693"
ORIGIN      
        1 mnldslsftl sqisylvanl nkknfrdscq eisvlvqwkg leadrhllrs llscidfstg
       61 eppessakdy fqvqllkqec tnllskpsfi snlcfaidhp fhqqktlnps pkfflrlkkv
      121 lgltlvqeva faiallhsen teirtlaleh vkkqlpelik nyinsetsnk hqeeglhdsl
      181 pevlhlilsq afhsanqfgl sseakekflk nlrrdfprel vpvvlaplly pdngetsqak
      241 ielnmavnqm dgnslvelim elgygftssv decrsalagl gareispaca arvlahmars
      301 ynslddaggl qsfwgnsgat qdankeksad svapttwnve vfiqalkeiq stlswneviv
      361 kldhaefvvk drqglsllit glrmglqhqg yppdmfpvel fyrhwdnveg qfslvqqilk
      421 cpdifcfady pyhsvtvdvl kaapesdske aqtwrslnvv elllhmaerg lygpvqeifk
      481 wpiqhcpdvl vlallqinap vtilrqells tlmpiflgnh pnsavilhha whgntvkiks
      541 iimhamaewy irgdhdqtrl srildvaqdl kalthlltaq sypfvidlac lasrreylkl
      601 ekwltdkire qgevfvaacv kflqrrcpqv mgpgikedit vpkasqlpqe tlttmlaclq
      661 vcagsvsqec sdaimtmvqn cslmlskstm srppppgvlr hrglepfnpa tlggqlfssk
      721 qvdplgnlss slasmglgss lgppsssafn lpgalgplvs apgspsrlmg pspspfpmlp
      781 lssqlhsgpv gtqgpsvlpg sttigglgrl gpptgiekar ipetsnlfpd msqnvskeie
      841 deansyfqri ynhpphptls idevldmlkk fqdsgskrer evfncmlrnl feeyrffpqy
      901 pdkelqitaq lfggiiergl vnsymtlgla lrfvldalrk pegskmyyfg italdrfksr
      961 lkdyqtyceh vraiqhfsef pphlieyiey glqgqepptr pqgpvlpktl aamlapvttp
     1021 yktittttit tsttqaksst tpttslsarp sianatnidt llvatdkeek itsppealqd
     1081 ktafifnnls qlnlqqkcde ireivteeyw pwmaqylvmk rasielnfha lysnfldclk
     1141 lpevnkmvtr etfrnikvll rsdkgianfs drsllknlgh wlgmltlgrn kpilqididl
     1201 ksllveayhk gqqellyvvp fvakvlesca ksrvfrppnp wtmaimnvla elhqepdlkl
     1261 nlkfeievlc knlsidvgel kpalylkdpe klrnleyqls hpnkksesnn nqqqtqgpie
     1321 elvgstttgt ivpqtapant tpslptgppe prfnymdisv tgianisqhi tinnqlplfq
     1381 thphlkqfvr paveraiqew ihpvvdrsik ialttseqiv rkdfaldpee vrmrtaarhm
     1441 vrnltagmam itcrdqvmas istnlkqafl tammgttpqq kelaeqaanv vaadnmelac
     1501 afvqktaiek aipemdkrll neielrkiar qegrrycdpl akyqaermpe qirlkvggvt
     1561 aqqmavyeef arnipgflpl serdtqallm pkpvteptvt afaanpavav ataqqvaaya
     1621 aavsndevga mleklaaeve vllaamgpav pppqhaalhs llesiiltrr srdagaamtl
     1681 lkkaveglld gpsissgvid seiilryrel hlrilkclqd praygmqwtn khvtrcltec
     1741 reefrynfea vdylirshli slpqydlala qaidsgnama tafamqlvql yliderqtth
     1801 vtesdlfhti eilarmahhr appegillfr ltslieslra nhdpgvladr apagptahih
     1861 sgilqardfd dppglmekte yllrewvsmh hspthardpt kafgmfvhqm nihgilktdd
     1921 litrffklst qmcvdlcyra lsetsaapsv vrakcfhsld afvrlvallv khsgdstnth
     1981 tkinllnkvl givagvllqd heirgtdfqq lpyhrifiml flelcapepv leavnyqvlt
     2041 afchtlhilr pakasgfcya wlelvshrvf igrmlaitpq qkcwgmyaql lidlfkylap
     2101 ylrnaelakp vtllykgtlr vllvllhdfp eflcdyhygf cdvippnciq mrnlilsafp
     2161 rnmrlpdpft pnlkvdmlqe iahaprvltn fasmiqplsf kkeldsylka rapvtflsel
     2221 rsnlqvsqea gvryniqlmn alvlyvgtqa iayirskght pnmstiahsa hmdifqnlav
     2281 dldtegrylf lnaianqlry pnshthyfsc tllylfaean teaiqeqitr vllerlivnr
     2341 phpwgllitf ieliknptyk fwthefvhca peieklfesv arscmvqkqv qptpepdipe
//
LOCUS       XP_034176929            1908 aa            linear   INV 03-SEP-2025
DEFINITION  jumonji, AT rich interactive domain 2 [Osmia lignaria lignaria].
ACCESSION   XP_034176929
VERSION     XP_034176929.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321038.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176929.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1908
                     /product="jumonji, AT rich interactive domain 2"
                     /calculated_mol_wt=214732
     Region          1300..1341
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          1364..1475
                     /region_name="ARID_JARD2"
                     /note="ARID/BRIGHT DNA binding domain of Jumonji/ARID
                     domain-containing protein 2 (JARID2) and similar proteins;
                     cd16870"
                     /db_xref="CDD:350634"
     Site            order(1388..1394,1419,1421..1422,1425,1436,1438..1440,
                     1442)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350634"
     Region          1617..1732
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          1832..1886
                     /region_name="zf-C5HC2"
                     /note="C5HC2 zinc finger; pfam02928"
                     /db_xref="CDD:460750"
     CDS             1..1908
                     /gene="Jarid2"
                     /coded_by="XM_034321038.2:459..6185"
                     /db_xref="GeneID:117602707"
ORIGIN      
        1 mvlsrndkrk rkegdlvdlm eplsespkrt kvhaqrkfaq gatnvfnpsh tpikdkekak
       61 pavitelith krpntedflt flcfrgtsil ppslnffnvg nkkekpepkp lrastdkvic
      121 ssttssenqk sdssqkivtk vkpaindkkk vsgsiqknit tfksatstvq alkkkyqeqr
      181 lakqriknkf ktscvmrtrs ctertmlkst nqlpprkflp rietkrlglr ssvladktkk
      241 tspkvksapp kpkkkviakq nssdtstses tsedeeeegp seksvsqikr taqknvqkni
      301 ctrsksemrr itrshsgtvs pknlcrrptr ktkeaaavym eilgrklisp dlenddnvsm
      361 dsfpelpnar ksaqtenevk skakqsvskp vtknkdgkit gihsvsnkrl dktkvskiaf
      421 kskrlirvqk yceidsdees vsssndtsnt kpvtrrslgk lnkptsrylr sstkkvvtri
      481 evqnnvniks ksqvqnnlrf nkekfvikrk reqnfskssp ekgselkqke gkiqnnahdi
      541 tdsdeetlgm llnklkrkkd dpppneeisp nnnnssnnnn nnnninnndn sncdvidkve
      601 hsldensvkv dlskvsddee sfrgftkkti skvlnscqth ananllvtek stdtskttnt
      661 edvkdsdasn qqenkelinn dpclhktspt tsvpnnqsvq seriqktkle ftkhiekech
      721 hkpemsgnnv dmslstlhvr kekvnmsteq iekwlnessf akeeskleme nvsafkydvc
      781 ekktdvshls istkiqhlvr pvnvtlskla ekanakdrin mqnkcisitt mdvksidvkq
      841 eneitivnkn taelkdinkt rnvksskdsc ageendialk cdqnvvdvtg ekklsgekkt
      901 ifqprkpflp kvkerktvip nanafspene ssvyafesdt eipvntpfrr kvrestrsnm
      961 aialsesneq kaesdtikse nntnknveki itddceitel kensntvllp keseaietkn
     1021 vpnatlnink felpknfatl aniqvlpldk ltttwsnvnc sasiavqvnl ddnaqsqehg
     1081 regdsnphks teistqtesn nenddendgq lfyiplqavt rngpnlvqgq qliqgvavkl
     1141 gtegptgpnq kvllraklvt kppasiarcp pvgtvqpttr tppnstfitt dhstpstsss
     1201 aasiitapip etqssspckn enviqtvnrq nigilsnvgt ekltkspkts rerktsidsn
     1261 kngkrcqiks kqkssevysp snnvtfpnak dvnseaclve aptfhpsekd fqdpleyidk
     1321 irpvaekfgi crvvpppnfk peckvsddmr ftaynqyvhr mlhrwgpnvk emmaikkyla
     1381 tqsitlthpp wiggmevdlp hlyqtvqslg glkeviekkk wqkvadgmki pksaqdrvtk
     1441 lddiyckyll pydtlspeer gklfdevese wikkesralq rqnapvtene ededddssde
     1501 ieecivkgrn mplnafyria rntqrmwfge nqrsgneaeg asadevesaf wkhvaerkrh
     1561 vcvhaasids sgrgfgfsva knspfarhpw nlkvltnnag svlralgpim gvtvptlhvg
     1621 mlfsaccwyr dphglpwiey lhtgakkiwy gipdehnnhf realskmvpr ycknktiwlp
     1681 sdtamvppel lvsngvslcq tvqepgqfii vfpkaftssi ctgyvvsesv yfaqlswlet
     1741 aeqvfkdiqd scepsifsfe rllfniinds rshievlkqi lpsvikirek eisyrkqlvs
     1801 vglinterlp lpdsgkrkkg kkvkeddgdf eceicranlf vslvsnsqdd siyclphalq
     1861 lfnkkkqvlk hctlmytyne delddlihkl gerieakskk tnqikqtk
//
LOCUS       XP_034176940            1367 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602715 [Osmia lignaria lignaria].
ACCESSION   XP_034176940
VERSION     XP_034176940.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321049.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176940.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1367
                     /product="uncharacterized protein LOC117602715"
                     /calculated_mol_wt=142524
     Region          273..>738
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     Region          <759..>1047
                     /region_name="Glutenin_hmw"
                     /note="High molecular weight glutenin subunit; pfam03157"
                     /db_xref="CDD:367362"
     Region          <1090..1320
                     /region_name="PHA03253"
                     /note="UL35; Provisional"
                     /db_xref="CDD:223025"
     CDS             1..1367
                     /gene="LOC117602715"
                     /coded_by="XM_034321049.2:139..4242"
                     /db_xref="GeneID:117602715"
ORIGIN      
        1 mgmmrlifvi licfsgtvfs kyyekrrfdr siqansepsi vkkswiftds ethyraykdk
       61 qenncylekl ddnvvseeyp ntkfqqrnri sktfyvsdet ltkleawqla ggrivdfcrg
      121 rniillqnti pipektvdpf fnilpydend iatrrvadiv ltplinrakr qielekrqel
      181 nkyfnqqrsr reaqqpqgkf rgqtqsqyls igtddqkegk aeaeatqqss rsvvsgsrgm
      241 gqaqsmsfgs igcedcskyt setapdryiq hpgtgtgyar tslpkqdgtt yphdltpstl
      301 ikdgrrpysg gvsypvvpgs vpgttgtggg tvypggipgt tsgggtvypv gvpgttttdg
      361 gtvypssvpg sttgggavyp agvpgsttgg gtvypggvpg tttdggvvyp grvpgttttg
      421 ggtvypggip gtttgggvvy pggvpgtttg ggivhpggvp gtttgggvvy pgrvpgtttg
      481 gsmvhpggvp gtttgggvvy pggvpgtttg ggviypggvp gttigggviy pggvpgtrtg
      541 ggvvypggip gtttgggvvy pgsvpgtttg ggvvypgrvp gttttgggtv ypggipgtat
      601 gggvvypsti pgitstggvv ypgstggqie sksvadatai yrngqipgak fpgevmyptv
      661 dgggingdri rysgvpapgt lpsdtadrrt vtgypdgtsl ppgqlretvp gsqplgtvsk
      721 ggstytggti ptsvgtanly pgtagqqidt haiipsgyph qgqypaypqq gqypvypqqg
      781 qypaypqqgq ypaypqgqyp gsliwqngek piaqqypseg qnirqypesq ypskvglatn
      841 agetsqyyqq pgtlpsgedd nsnsqasstv kqtdagtqas asaqgkfgqg taqsqvtgty
      901 sgsgsfsaqa gstdvnksvq teinggkesv tsnaqgtggy gksqaqvqld sesgatstga
      961 qssgwnhgtn sqvqasskgg madaqangeg stssqaqigf qpylktdekv erhsrpyrgg
     1021 gtasaqsgth rgqsqsqleg sfqygitytg aaqagsgsga aasrkpfnfn ltdtepfklf
     1081 kpsyipqivk nnnsetents sdtdyeynqd klqqglqtss ssqkavfikq stnensknvg
     1141 ntqtqfntes qtddmlddyk dydaeeddwe erninhqsqa vhlatgnqyd ihvkqdtnta
     1201 qvgdmlqpgq svsgytippg frgrvtsvag setiahgdgk sqsqsislvp vepnvshgnk
     1261 spisetrslk tnrerlaedy vssnienskp tanipmkpsy ytvtnsvagk idgsrnsprk
     1321 yehryytkss tcgyftfscn ivygsngrtk ickpkiptnp dgtamkc
//
LOCUS       XP_034176962            1076 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit A [Osmia
            lignaria lignaria].
ACCESSION   XP_034176962
VERSION     XP_034176962.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321071.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1076
                     /product="eukaryotic translation initiation factor 3
                     subunit A"
                     /calculated_mol_wt=128197
     Region          426..>494
                     /region_name="PINT"
                     /note="motif in proteasome subunits, Int-6, Nip-1 and
                     TRIP-15; smart00088"
                     /db_xref="CDD:214509"
     Region          <627..>888
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          927..>1054
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     CDS             1..1076
                     /gene="eIF3a"
                     /coded_by="XM_034321071.2:156..3386"
                     /db_xref="GeneID:117602725"
ORIGIN      
        1 marygqrpen alkranefie vgkpsraldt lqevfrnkkw tynwsesvle pimfkyldlc
       61 velkkshiak eglfqyrnmf qsvnvgslen virgylrmae ektnqarkqs qqavididdl
      121 dnlatpesil lsavsgedaq drsdrtiltp wvkflwesyc qclellrtna hvetlyhdia
      181 rmafqfcley nrktefrklc eklrkhleei cklpalvsnv smnraetqql nletrlnqld
      241 saiqmelwqe afkssedvhg mmnlskklpv pktmanyyqk lamvfwkagn ylfhaaalfk
      301 llqlsremkk nmsleeqqrm anrvllatls iplpsahpef drfietdksp lekaqklatl
      361 lgltqpptrv sllkdivrln ivnlaspqlq elyswlevef hplelcnrvd sviqtlqade
      421 nsplaqyvpa lqdvtlvrlv hqisqvyqti qfarllelak fttdfhlerl lvdcvryndm
      481 qirinhgkkc vhfgvdlsea qredhpdgpv lqampseqir cqlvnmatvl hrainiinpn
      541 kkktereklr tamvthyhet klkehqrilg rhkiieerke yiehintvre eeemrrqeel
      601 qrqqmlaeqk rleqereere rkrqqseiqq ikdrhlkekm qqisqtshgq kvlkkldede
      661 ikkldaeqia areaeelqke rremqqklks qekkidyler akrleeipll ekaveekmel
      721 akqrweqqea erialaieer qqavatrerm armkedhdsf lakilaerks iyleklkefe
      781 kvlneeragr llkrkmerka erkakweker aeaierrrqe earikqeeek rrmeeerakr
      841 eeeerlkrae eeakeaerla kierqaeitr areaeierkl eeerqrekem sdtwrrgaeq
      901 rthrvvqdkp memswrrpid ikddgvkdds srwkrretad kwrkdeekpk ketgdrwrkd
      961 dfdkddtrer dridkdrgmd grsvnmrdsl rdpvsdssrg grgrlperrt hrredtvrnt
     1021 ggqnwrdkvd tiqnsprdpp krdekreepk edrlppkhee rrrppeddgw sqvsrr
//
LOCUS       XP_034188491             905 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608049 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188491
VERSION     XP_034188491.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332600.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188491.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..905
                     /product="uncharacterized protein LOC117608049 isoform X1"
                     /calculated_mol_wt=104468
     Region          72..111
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          <161..>302
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          189..218
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          219..243
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          244..272
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          273..296
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          336..396
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          397..422
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          461..885
                     /region_name="PqqL"
                     /note="Predicted Zn-dependent peptidase, M16 family
                     [General function prediction only]; COG0612"
                     /db_xref="CDD:440377"
     CDS             1..905
                     /gene="LOC117608049"
                     /coded_by="XM_034332600.2:272..2989"
                     /db_xref="GeneID:117608049"
ORIGIN      
        1 mqgeektvne qvfpianhli cymckkkmkm krsrslspka scseddmkrm klgenekndi
       61 iimhphildf sddvllnifk ylnpqdlmai slscqrlaqv aqdktlwrrv dfrvkpilln
      121 dlkeyikffq pmtvslavrg slhcekeaev spyffnsikn sctqlrelti eeyyingdki
      181 qitdfprtie klslegckmg ylrgnksyff kmnfhmpnlt clilsncqwf tphsllvisk
      241 mpklkelrln schrlgecva ytslatrfgf ktleildlrd talgdsevgc fsctktlthl
      301 ylecpfnlrn tessdqesrp lppeglnqpv yeddnlvqfv vedgfrweny tfgrclitdr
      361 aicalgidtc drkiinnsae gvivveddkr ifnnphlktl vvrnypyvtn sslvhlalna
      421 ssleyldgyi smsngcykqf eaykgisrnm aklsnkssns eqiescivss gmrlvcecrn
      481 sftttlgcfl pagamyempk ergsaifleh llfrrtqhnn qeqlekllge iggkvaaiam
      541 rdmflfygtv lphevdkliq lfadvilngi icdedverek cvilyelskm aldkekvvmd
      601 ylptvayqdt alangvypet dtikkfcten liefrdrlfk tyfmtmvctg siglekfkrl
      661 vckhftieed dnelpidlan nvqsciesfq crfsgcdlrl rdddeelgyv aigfegpsyk
      721 epedhliltv akeivgswdk scsgashnap yiahcafnte fcymyksffh nwaqctsmwg
      781 cyfvsnkldq enmvdllqre wmklcttitq kevlravnqc klkdlivlsd ptsrffdivq
      841 yifrygyyep iqnrileyek ittdklketc ekyiydqnpt vvaigrienl phyyvirngm
      901 yllry
//
LOCUS       XP_034188582             878 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608049 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188582
VERSION     XP_034188582.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332691.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188582.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..878
                     /product="uncharacterized protein LOC117608049 isoform X2"
                     /calculated_mol_wt=101303
     Region          45..84
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          <134..>275
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          162..191
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          192..216
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          217..245
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          246..269
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          309..369
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          370..395
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          434..858
                     /region_name="PqqL"
                     /note="Predicted Zn-dependent peptidase, M16 family
                     [General function prediction only]; COG0612"
                     /db_xref="CDD:440377"
     CDS             1..878
                     /gene="LOC117608049"
                     /coded_by="XM_034332691.2:358..2994"
                     /db_xref="GeneID:117608049"
ORIGIN      
        1 mkmkrsrsls pkascseddm krmklgenek ndiiimhphi ldfsddvlln ifkylnpqdl
       61 maislscqrl aqvaqdktlw rrvdfrvkpi llndlkeyik ffqpmtvsla vrgslhceke
      121 aevspyffns iknsctqlre ltieeyying dkiqitdfpr tieklslegc kmgylrgnks
      181 yffkmnfhmp nltclilsnc qwftphsllv iskmpklkel rlnschrlge cvaytslatr
      241 fgfktleild lrdtalgdse vgcfsctktl thlylecpfn lrntessdqe srplppegln
      301 qpvyeddnlv qfvvedgfrw enytfgrcli tdraicalgi dtcdrkiinn saegvivved
      361 dkrifnnphl ktlvvrnypy vtnsslvhla lnassleyld gyismsngcy kqfeaykgis
      421 rnmaklsnks snseqiesci vssgmrlvce crnsftttlg cflpagamye mpkergsaif
      481 lehllfrrtq hnnqeqlekl lgeiggkvaa iamrdmflfy gtvlphevdk liqlfadvil
      541 ngiicdedve rekcvilyel skmaldkekv vmdylptvay qdtalangvy petdtikkfc
      601 tenliefrdr lfktyfmtmv ctgsiglekf krlvckhfti eeddnelpid lannvqscie
      661 sfqcrfsgcd lrlrdddeel gyvaigfegp sykepedhli ltvakeivgs wdkscsgash
      721 napyiahcaf ntefcymyks ffhnwaqcts mwgcyfvsnk ldqenmvdll qrewmklctt
      781 itqkevlrav nqcklkdliv lsdptsrffd ivqyifrygy yepiqnrile yekittdklk
      841 etcekyiydq nptvvaigri enlphyyvir ngmyllry
//
LOCUS       XP_076546241             878 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608049 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546241
VERSION     XP_076546241.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690126.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..878
                     /product="uncharacterized protein LOC117608049 isoform X2"
                     /calculated_mol_wt=101303
     Region          45..84
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          <134..>275
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          162..191
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          192..216
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          217..245
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          246..269
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          309..369
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          370..395
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          434..858
                     /region_name="PqqL"
                     /note="Predicted Zn-dependent peptidase, M16 family
                     [General function prediction only]; COG0612"
                     /db_xref="CDD:440377"
     CDS             1..878
                     /gene="LOC117608049"
                     /coded_by="XM_076690126.1:291..2927"
                     /db_xref="GeneID:117608049"
ORIGIN      
        1 mkmkrsrsls pkascseddm krmklgenek ndiiimhphi ldfsddvlln ifkylnpqdl
       61 maislscqrl aqvaqdktlw rrvdfrvkpi llndlkeyik ffqpmtvsla vrgslhceke
      121 aevspyffns iknsctqlre ltieeyying dkiqitdfpr tieklslegc kmgylrgnks
      181 yffkmnfhmp nltclilsnc qwftphsllv iskmpklkel rlnschrlge cvaytslatr
      241 fgfktleild lrdtalgdse vgcfsctktl thlylecpfn lrntessdqe srplppegln
      301 qpvyeddnlv qfvvedgfrw enytfgrcli tdraicalgi dtcdrkiinn saegvivved
      361 dkrifnnphl ktlvvrnypy vtnsslvhla lnassleyld gyismsngcy kqfeaykgis
      421 rnmaklsnks snseqiesci vssgmrlvce crnsftttlg cflpagamye mpkergsaif
      481 lehllfrrtq hnnqeqlekl lgeiggkvaa iamrdmflfy gtvlphevdk liqlfadvil
      541 ngiicdedve rekcvilyel skmaldkekv vmdylptvay qdtalangvy petdtikkfc
      601 tenliefrdr lfktyfmtmv ctgsiglekf krlvckhfti eeddnelpid lannvqscie
      661 sfqcrfsgcd lrlrdddeel gyvaigfegp sykepedhli ltvakeivgs wdkscsgash
      721 napyiahcaf ntefcymyks ffhnwaqcts mwgcyfvsnk ldqenmvdll qrewmklctt
      781 itqkevlrav nqcklkdliv lsdptsrffd ivqyifrygy yepiqnrile yekittdklk
      841 etcekyiydq nptvvaigri enlphyyvir ngmyllry
//
LOCUS       XP_034188671             864 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial-processing peptidase subunit beta isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034188671
VERSION     XP_034188671.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188671.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..864
                     /product="mitochondrial-processing peptidase subunit beta
                     isoform X3"
                     /calculated_mol_wt=99615
     Region          72..111
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          420..844
                     /region_name="PqqL"
                     /note="Predicted Zn-dependent peptidase, M16 family
                     [General function prediction only]; COG0612"
                     /db_xref="CDD:440377"
     CDS             1..864
                     /gene="LOC117608049"
                     /coded_by="XM_034332780.2:279..2873"
                     /db_xref="GeneID:117608049"
ORIGIN      
        1 mqgeektvne qvfpianhli cymckkkmkm krsrslspka scseddmkrm klgenekndi
       61 iimhphildf sddvllnifk ylnpqdlmai slscqrlaqv aqdktlwrrv dfrvkpilln
      121 dlkeyikffq pmtvslavrg slhcekeaev spyffnsikn sctqlrelti eeyyingdkc
      181 lilsncqwft phsllviskm pklkelrlns chrlgecvay tslatrfgfk tleildlrdt
      241 algdsevgcf sctktlthly lecpfnlrnt essdqesrpl ppeglnqpvy eddnlvqfvv
      301 edgfrwenyt fgrclitdra icalgidtcd rkiinnsaeg vivveddkri fnnphlktlv
      361 vrnypyvtns slvhlalnas sleyldgyis msngcykqfe aykgisrnma klsnkssnse
      421 qiescivssg mrlvcecrns ftttlgcflp agamyempke rgsaiflehl lfrrtqhnnq
      481 eqlekllgei ggkvaaiamr dmflfygtvl phevdkliql fadvilngii cdedverekc
      541 vilyelskma ldkekvvmdy lptvayqdta langvypetd tikkfctenl iefrdrlfkt
      601 yfmtmvctgs iglekfkrlv ckhftieedd nelpidlann vqsciesfqc rfsgcdlrlr
      661 dddeelgyva igfegpsyke pedhliltva keivgswdks csgashnapy iahcafntef
      721 cymyksffhn waqctsmwgc yfvsnkldqe nmvdllqrew mklcttitqk evlravnqck
      781 lkdlivlsdp tsrffdivqy ifrygyyepi qnrileyeki ttdklketce kyiydqnptv
      841 vaigrienlp hyyvirngmy llry
//
LOCUS       XP_034176977             901 aa            linear   INV 03-SEP-2025
DEFINITION  pyruvate dehydrogenase phosphatase regulatory subunit,
            mitochondrial [Osmia lignaria lignaria].
ACCESSION   XP_034176977
VERSION     XP_034176977.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321086.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176977.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..901
                     /product="pyruvate dehydrogenase phosphatase regulatory
                     subunit, mitochondrial"
                     /calculated_mol_wt=101582
     Region          70..431
                     /region_name="DadA"
                     /note="Glycine/D-amino acid oxidase (deaminating) [Amino
                     acid transport and metabolism]; COG0665"
                     /db_xref="CDD:440429"
     Region          417..472
                     /region_name="FAO_M"
                     /note="FAD dependent oxidoreductase central domain;
                     pfam16350"
                     /db_xref="CDD:465100"
     Region          466..815
                     /region_name="GcvT"
                     /note="Glycine cleavage system protein T
                     (aminomethyltransferase) [Amino acid transport and
                     metabolism]; COG0404"
                     /db_xref="CDD:440173"
     CDS             1..901
                     /gene="LOC117602729"
                     /coded_by="XM_034321086.2:1..2706"
                     /db_xref="GeneID:117602729"
ORIGIN      
        1 msiimwhysk sfynknitkw snnvfcnyqr irhsssksnl etnedvddpl pkeakvvicg
       61 ggvmggavay hlslmglgpq tviveserlg ggttwhtsgl vgafkptlaq vklaqdsial
      121 ykelekkglp tgwkqcgsls lartrdrmtv frrmkaesis rniechlitp kqaqeicpmv
      181 riddlagglw ipgdgvgnpy qicltlikea kekgvkvfen ckvtkivtqd dkieavetth
      241 gtiecehfvn cagfwarnvg klsepfvkvp lhpvehyylh tkpitgldpm tpvirdldgy
      301 iyfrendgrl laggfepiak pvfedgkipe steqrflped wdhfhilleq mlhrvpslgn
      361 vileklcngp eafspdckwi vggapeirny yiaagmktvg isaaggvgra taelivhgst
      421 sldmyeldvs rflglhnnrk flrdrvkevp gmhyalqyph nefktgrnlr mspiypklre
      481 agavfgqvmg yerpswfqld kdddfettng fqkckiaytn tfskppwfep vseeyaacre
      541 rvglsdyssf tkidlwstgm evvellqylc sndvdvpigs iihtgmqnhc ggyendcsla
      601 riapnhymmi aptiqqtrck qwinrhlppd gsvsvsdvts aytaicimgp atrqllselt
      661 dtdlnpknfp fftfreldvg langirtmnl thtgelgyvl yipnefalhv ytrlidagak
      721 ygirhagyya tralrvekfy afwgqdldtf ttplecgraw rvkldkdinf igrnallkqr
      781 eegvkrkyvq lllndhdley dtwcwgsepi frngqycgmt tttgygftfk kqvclgfvqn
      841 idsegrpqev tneyilsgdy evdvagikfp akchlhspnl ptkfpdkerd syhatrdhqa
      901 a
//
LOCUS       XP_034176992             887 aa            linear   INV 03-SEP-2025
DEFINITION  nonribosomal peptide synthetase ebony isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034176992
VERSION     XP_034176992.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321101.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176992.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..887
                     /product="nonribosomal peptide synthetase ebony isoform
                     X1"
                     /calculated_mol_wt=100080
     Region          38..557
                     /region_name="A_NRPS"
                     /note="The adenylation domain of nonribosomal peptide
                     synthetases (NRPS); cd05930"
                     /db_xref="CDD:341253"
     Region          <130..>194
                     /region_name="PLN02479"
                     /note="acetate-CoA ligase"
                     /db_xref="CDD:178097"
     Site            order(177,180..185,187..188)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341253"
     Site            order(180..181,301..302,326..331,356,447,458,461,551)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341253"
     Region          <610..653
                     /region_name="PP-binding"
                     /note="Phosphopantetheine attachment site; pfam00550"
                     /db_xref="CDD:425746"
     CDS             1..887
                     /gene="e"
                     /coded_by="XM_034321101.2:796..3459"
                     /db_xref="GeneID:117602735"
ORIGIN      
        1 mgtilqqsvl kgrqtnaske kdllhtlfkq tvennssqpa iyyedddgkv ytityeelnl
       61 ltnrlarvlq kcekpenssq sfvavcmkps hrlpamllai ikagmaylpl dvdfpmsrmk
      121 hvlqeakpfv vlveeeadps ifegtktvty eqlleqsrhe gqedleieed pertaivlyt
      181 sgstgtpkgv llshatvlnr lrwqwrelpy ksdeqycifk tsltfvdsvs eiwgsllqgr
      241 clvvvpkhvt rdpekfvqvl ekhkiqrlvl vpsllysmlm ylslrdkeni lrslrlwics
      301 getlpvalad efftrfahdd nkilanfygs teimgdvtyh lindqtqlqs mekvpigkrn
      361 ftilyfsdnr sliterkndi sgkpldncii yivnkemrlv pqgevgeliv agrnlaagyi
      421 rdndshkfqd nphaidpeys rvyctgdyar iskgmviyeg rmdsqikirg hrvdltevek
      481 avskvpgidn vvvlcykpge lsqtllafvt vaagscscgk kiegilqatl ppymlprviv
      541 vdgiplltng ktdrqallkh yessnlnnen eefvncdytg vpdedlakar vlfptvasvi
      601 ggggggrsnv kldanfyelg gnslnsiytv tklrdqgyei gitefitakd laevlsrmri
      661 saddvspenn inedeytfem lndshkkdai eiitesfysk adleqwlmpg iakddyrimm
      721 eilweplvek nlsfavksar dgrtiavgln fdlwdepeli ldskltivfe flehleapir
      781 erklpkgkgq iihnfmmtts sdlnpaenvi mmkameeycl klakrkeyag ifttntsplt
      841 qqlgtdvfgy dtmltyqvnk ycapdgtkpf geapdnqlai cslkmiv
//
LOCUS       XP_034177001             862 aa            linear   INV 03-SEP-2025
DEFINITION  nonribosomal peptide synthetase ebony isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177001
VERSION     XP_034177001.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321110.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177001.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..862
                     /product="nonribosomal peptide synthetase ebony isoform
                     X2"
                     /calculated_mol_wt=97110
     Region          38..532
                     /region_name="A_NRPS"
                     /note="The adenylation domain of nonribosomal peptide
                     synthetases (NRPS); cd05930"
                     /db_xref="CDD:341253"
     Site            order(177,180..185,187..188)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341253"
     Site            order(180..181,301..302,326..331,356,422,433,436,526)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341253"
     Region          <585..628
                     /region_name="PP-binding"
                     /note="Phosphopantetheine attachment site; pfam00550"
                     /db_xref="CDD:425746"
     CDS             1..862
                     /gene="e"
                     /coded_by="XM_034321110.2:792..3380"
                     /db_xref="GeneID:117602735"
ORIGIN      
        1 mgtilqqsvl kgrqtnaske kdllhtlfkq tvennssqpa iyyedddgkv ytityeelnl
       61 ltnrlarvlq kcekpenssq sfvavcmkps hrlpamllai ikagmaylpl dvdfpmsrmk
      121 hvlqeakpfv vlveeeadps ifegtktvty eqlleqsrhe gqedleieed pertaivlyt
      181 sgstgtpkgv llshatvlnr lrwqwrelpy ksdeqycifk tsltfvdsvs eiwgsllqgr
      241 clvvvpkhvt rdpekfvqvl ekhkiqrlvl vpsllysmlm ylslrdkeni lrslrlwics
      301 getlpvalad efftrfahdd nkilanfygs teimgdvtyh lindqtqlqs mekvpigkpl
      361 dnciiyivnk emrlvpqgev gelivagrnl aagyirdnds hkfqdnphai dpeysrvyct
      421 gdyariskgm viyegrmdsq ikirghrvdl tevekavskv pgidnvvvlc ykpgelsqtl
      481 lafvtvaags cscgkkiegi lqatlppyml prvivvdgip lltngktdrq allkhyessn
      541 lnneneefvn cdytgvpded lakarvlfpt vasviggggg grsnvkldan fyelggnsln
      601 siytvtklrd qgyeigitef itakdlaevl srmrisaddv spenninede ytfemlndsh
      661 kkdaieiite sfyskadleq wlmpgiakdd yrimmeilwe plveknlsfa vksardgrti
      721 avglnfdlwd epelildskl tivfeflehl eapirerklp kgkgqiihnf mmttssdlnp
      781 aenvimmkam eeyclklakr keyagifttn tspltqqlgt dvfgydtmlt yqvnkycapd
      841 gtkpfgeapd nqlaicslkm iv
//
LOCUS       XP_034177015             866 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602743 [Osmia lignaria lignaria].
ACCESSION   XP_034177015
VERSION     XP_034177015.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321124.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..866
                     /product="uncharacterized protein LOC117602743"
                     /calculated_mol_wt=95699
     Region          <102..256
                     /region_name="PHA02682"
                     /note="ORF080 virion core protein; Provisional"
                     /db_xref="CDD:177464"
     CDS             1..866
                     /gene="LOC117602743"
                     /coded_by="XM_034321124.2:1708..4308"
                     /db_xref="GeneID:117602743"
ORIGIN      
        1 mtpmvplils tlllaitcsl fcsasgdggp nspifnvdnt aleknlaaif nkvahssatt
       61 krsvpaydaq vsasttlstv pspltttttt ttttttttta tpsipsaryp pvpratvspv
      121 frelpsyapp sralftpplp peylhpfank ptlrgtnsda hsglkrpvpp psgtpaheri
      181 pirppdlqap sqiperhshs rnkplntpsk eqnivessel drrnntnefv ptlhypsisr
      241 ilsgsngrkq dipeillkpl atktppqnap vapttekmtt lstttsrtps tldssksvtq
      301 ptifnlpntr rhdddgyhgl rnengfkten ieivdterlp ypqpqppapa pkrpdesgdh
      361 ivpqvdthhp lestwriawf lhvyvaailf tlmalysiyk ivrfneatnl ltqsyfltvh
      421 lllttvctlr cfhlfydayn lghslpepls rvlmylpapl lstafatlvl ylarcseaps
      481 lnnrfltpaa lllfstvhiv lcvslhvsth vlgyqdeaki lplicqciyi ivcvtlglgy
      541 myvyrvvrsq illtnnktag anhivgadpt tmalstaitt siatallfil mgfvqlygif
      601 gnvqeqpqsy pwlwwgwqfs vrlleltvcg llawvaslsq yplrekqlqq hpvhsgfalf
      661 pcgsststen mddvlypaic stnqaiqnyt mrtgkqvydd sfplntlpeh lnisgtferh
      721 sirksgtmgh fphegrgsln rhgngiqtlr ssesrgrhtp vqgvheqtat tgstmlvaed
      781 gfvrfrslga eddelsdtps vvgthgrgss lagsvrfnar hptlqhqhph qhqhqhqhqh
      841 qhqhqhslqh phqsqhqahh qlyint
//
LOCUS       XP_034177030             866 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602743 [Osmia lignaria lignaria].
ACCESSION   XP_034177030
VERSION     XP_034177030.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321139.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..866
                     /product="uncharacterized protein LOC117602743"
                     /calculated_mol_wt=95699
     Region          <102..256
                     /region_name="PHA02682"
                     /note="ORF080 virion core protein; Provisional"
                     /db_xref="CDD:177464"
     CDS             1..866
                     /gene="LOC117602743"
                     /coded_by="XM_034321139.2:1640..4240"
                     /db_xref="GeneID:117602743"
ORIGIN      
        1 mtpmvplils tlllaitcsl fcsasgdggp nspifnvdnt aleknlaaif nkvahssatt
       61 krsvpaydaq vsasttlstv pspltttttt ttttttttta tpsipsaryp pvpratvspv
      121 frelpsyapp sralftpplp peylhpfank ptlrgtnsda hsglkrpvpp psgtpaheri
      181 pirppdlqap sqiperhshs rnkplntpsk eqnivessel drrnntnefv ptlhypsisr
      241 ilsgsngrkq dipeillkpl atktppqnap vapttekmtt lstttsrtps tldssksvtq
      301 ptifnlpntr rhdddgyhgl rnengfkten ieivdterlp ypqpqppapa pkrpdesgdh
      361 ivpqvdthhp lestwriawf lhvyvaailf tlmalysiyk ivrfneatnl ltqsyfltvh
      421 lllttvctlr cfhlfydayn lghslpepls rvlmylpapl lstafatlvl ylarcseaps
      481 lnnrfltpaa lllfstvhiv lcvslhvsth vlgyqdeaki lplicqciyi ivcvtlglgy
      541 myvyrvvrsq illtnnktag anhivgadpt tmalstaitt siatallfil mgfvqlygif
      601 gnvqeqpqsy pwlwwgwqfs vrlleltvcg llawvaslsq yplrekqlqq hpvhsgfalf
      661 pcgsststen mddvlypaic stnqaiqnyt mrtgkqvydd sfplntlpeh lnisgtferh
      721 sirksgtmgh fphegrgsln rhgngiqtlr ssesrgrhtp vqgvheqtat tgstmlvaed
      781 gfvrfrslga eddelsdtps vvgthgrgss lagsvrfnar hptlqhqhph qhqhqhqhqh
      841 qhqhqhslqh phqsqhqahh qlyint
//
LOCUS       XP_034177024             866 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602743 [Osmia lignaria lignaria].
ACCESSION   XP_034177024
VERSION     XP_034177024.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321133.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..866
                     /product="uncharacterized protein LOC117602743"
                     /calculated_mol_wt=95699
     Region          <102..256
                     /region_name="PHA02682"
                     /note="ORF080 virion core protein; Provisional"
                     /db_xref="CDD:177464"
     CDS             1..866
                     /gene="LOC117602743"
                     /coded_by="XM_034321133.2:1563..4163"
                     /db_xref="GeneID:117602743"
ORIGIN      
        1 mtpmvplils tlllaitcsl fcsasgdggp nspifnvdnt aleknlaaif nkvahssatt
       61 krsvpaydaq vsasttlstv pspltttttt ttttttttta tpsipsaryp pvpratvspv
      121 frelpsyapp sralftpplp peylhpfank ptlrgtnsda hsglkrpvpp psgtpaheri
      181 pirppdlqap sqiperhshs rnkplntpsk eqnivessel drrnntnefv ptlhypsisr
      241 ilsgsngrkq dipeillkpl atktppqnap vapttekmtt lstttsrtps tldssksvtq
      301 ptifnlpntr rhdddgyhgl rnengfkten ieivdterlp ypqpqppapa pkrpdesgdh
      361 ivpqvdthhp lestwriawf lhvyvaailf tlmalysiyk ivrfneatnl ltqsyfltvh
      421 lllttvctlr cfhlfydayn lghslpepls rvlmylpapl lstafatlvl ylarcseaps
      481 lnnrfltpaa lllfstvhiv lcvslhvsth vlgyqdeaki lplicqciyi ivcvtlglgy
      541 myvyrvvrsq illtnnktag anhivgadpt tmalstaitt siatallfil mgfvqlygif
      601 gnvqeqpqsy pwlwwgwqfs vrlleltvcg llawvaslsq yplrekqlqq hpvhsgfalf
      661 pcgsststen mddvlypaic stnqaiqnyt mrtgkqvydd sfplntlpeh lnisgtferh
      721 sirksgtmgh fphegrgsln rhgngiqtlr ssesrgrhtp vqgvheqtat tgstmlvaed
      781 gfvrfrslga eddelsdtps vvgthgrgss lagsvrfnar hptlqhqhph qhqhqhqhqh
      841 qhqhqhslqh phqsqhqahh qlyint
//
LOCUS       XP_034177045             863 aa            linear   INV 03-SEP-2025
DEFINITION  mitogen-activated protein kinase kinase kinase 13 wallenda [Osmia
            lignaria lignaria].
ACCESSION   XP_034177045
VERSION     XP_034177045.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321154.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177045.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..863
                     /product="mitogen-activated protein kinase kinase kinase
                     13 wallenda"
                     /calculated_mol_wt=97045
     Region          155..391
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(155..158,161,163,174,176,198,214..217,260,264..265,
                     267,277..278)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..863
                     /gene="wnd"
                     /coded_by="XM_034321154.2:1..2592"
                     /db_xref="GeneID:117602756"
ORIGIN      
        1 mrtpaetetl sqpemykfes qeseapsggn qvvlspsrpe thiftnsmlc iqeelgqlgg
       61 iaggpiitdp hdaqlpnnsh sisgsnsnss etatkctpgs gtldaqrssw vegilgcmrp
      121 vwsmlskaav nekikghqtd dweipfetis elqwlgsgaq gavfsgklnk eivavkkvre
      181 pretdirhlr klnhpnivqf kgvctqapcy ciimefcpyg plydllrage pvppprlvsw
      241 skqiaagmay lhahkiihrd lkspnvligq gevvkisdfg tsrewneist rmsfagtvaw
      301 mapeiirnep csekvdiwsy gvvlwellsg evpykdvdss aiiwgvgnns lhlpipascp
      361 egyrllvkqc waakprnrps fkhieihlgi aavevlctkp ddyfktqqsw kkeirdhmkq
      421 mqtnscsspr feadlirrre delrhaqdir ehyerklert nnlylelsav llqleqrerd
      481 vikreqqngy kqckkrlvhp llkaqerlhr rrnptiqfst sstpttppsp tdgpqspaka
      541 tmytqlnesn qpetilapnn sfkqrkyrhr rvgsgcgvns sprssphher kstevcaryv
      601 dnqtqtdimd isetdnvrqt tvvsstekls vnsinkqsln kqvseclngn svlsddhyrm
      661 qsspcsspep snvnhangne rlkdcsdddn letlgrkvse iinanrlisp idngncddvi
      721 qhrgkedsvk lsghfcgiiv ngtniqqeqs eikvrpcnen inalcdhedd aceeswsdee
      781 gedpsytyny slrrrsiarr pigpgyrlrr lkhatvrieg vlasdeents eyshppssqs
      841 stlesnpdmq rvlrniqysh kvs
//
LOCUS       XP_034177076             605 aa            linear   INV 03-SEP-2025
DEFINITION  negative elongation factor B [Osmia lignaria lignaria].
ACCESSION   XP_034177076
VERSION     XP_034177076.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321185.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..605
                     /product="negative elongation factor B"
                     /calculated_mol_wt=67859
     Region          119..471
                     /region_name="COBRA1"
                     /note="Cofactor of BRCA1 (COBRA1); pfam06209"
                     /db_xref="CDD:461851"
     CDS             1..605
                     /gene="NELF-B"
                     /coded_by="XM_034321185.2:293..2110"
                     /db_xref="GeneID:117602765"
ORIGIN      
        1 mntvknscei gnglddlgip gqgflrdalt sctdplkaie efqlengill pslrpmlpll
       61 dlhgvrrldf hasvleelre klikrineig teradkgsag dkrlkellsk sfpavrvaal
      121 rpvvmcilrn tphiedkylr vlvrekelyn dadtevkrqi wkdnqslfgd evsplfsryi
      181 iekeqilfdh rnlnslffmp spkvrrqgev vqklahmigh svklydmvlq flrtlflrtk
      241 nihyctlrae llmalhdlev qdiisvdpch kftwcldaci reknvdikrs relqgfldsi
      301 krgqeqvlgd lsmilcdpya vnflassaik ialhlingea lprenavlvl llrmlalgls
      361 awqmidsqdf kepkldsqvv tkflpalmsl mvddqirqln cklppderes aiaiiehsgp
      421 ppdacqayaq lsgvaavlsm yyalhvgggg gvgrgrgdar glmrvlatlp ncqaqrafed
      481 pflhtlvsll ilnmadefsn esfctvifde fflaglgrdn vtrhllkllw yihpklpptr
      541 lhtlmkalqp tsqhneavhn lyealrdkig srsteeqltt aaqmdtlgld cppspltgvp
      601 tpgsl
//
LOCUS       XP_034177089             599 aa            linear   INV 03-SEP-2025
DEFINITION  putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034177089
VERSION     XP_034177089.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177089.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..599
                     /product="putative RNA polymerase II subunit B1 CTD
                     phosphatase RPAP2 isoform X1"
                     /calculated_mol_wt=68315
     Region          61..132
                     /region_name="RPAP2_Rtr1"
                     /note="Rtr1/RPAP2 family; pfam04181"
                     /db_xref="CDD:461212"
     CDS             1..599
                     /gene="LOC117602773"
                     /coded_by="XM_034321198.2:286..2085"
                     /db_xref="GeneID:117602773"
ORIGIN      
        1 mdrstlreei lerkkvkkkl skaqmqlaii kkkqcdakal tiveqllepk idsdwlldnl
       61 khinkshmed vieergitkl cgyvlcsnal ktiieqqyyi stkknkvydv tkrknfcssr
      121 cygasnyllq qmltsplwlr dkeeipvfkv lpakdeleks ipgdeiqfnd tivilnsdnk
      181 depaesntev dahdeenavk nsetvntyie lnnsempsvs keessknlde fgdikdvpid
      241 skgmidnvkq cmdnndddkk mftlensiil ddskiddtnn lsdtvsinkd adqsvkndqe
      301 knnvtiskqd tnisndgaqk mgkiqqnkpk rknvtkdkqs sefynlamni ehsikgwite
      361 dtisllsgee dtktrlleni iqhdrylhlc kklnklqled ekddntdvtk ntlkplphls
      421 vlqeegqkme lkvrafykgs tivedpknta kdveqgsdlv pvlplidsha pkalrrrifl
      481 dklnrilpdl lralasnklp qyiysneksa likalvntfs lsatniifkt aewtlvglvi
      541 ikmlsmidpq lkfllsskqa smyismilms ykldpnylnr lvmeltniki snidnttnl
//
LOCUS       XP_034177099             575 aa            linear   INV 03-SEP-2025
DEFINITION  putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034177099
VERSION     XP_034177099.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321208.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177099.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="putative RNA polymerase II subunit B1 CTD
                     phosphatase RPAP2 isoform X2"
                     /calculated_mol_wt=65418
     Region          37..108
                     /region_name="RPAP2_Rtr1"
                     /note="Rtr1/RPAP2 family; pfam04181"
                     /db_xref="CDD:461212"
     CDS             1..575
                     /gene="LOC117602773"
                     /coded_by="XM_034321208.2:271..1998"
                     /db_xref="GeneID:117602773"
ORIGIN      
        1 mqlaiikkkq cdakaltive qllepkidsd wlldnlkhin kshmedviee rgitklcgyv
       61 lcsnalktii eqqyyistkk nkvydvtkrk nfcssrcyga snyllqqmlt splwlrdkee
      121 ipvfkvlpak deleksipgd eiqfndtivi lnsdnkdepa esntevdahd eenavknset
      181 vntyielnns empsvskees sknldefgdi kdvpidskgm idnvkqcmdn ndddkkmftl
      241 ensiilddsk iddtnnlsdt vsinkdadqs vkndqeknnv tiskqdtnis ndgaqkmgki
      301 qqnkpkrknv tkdkqssefy nlamniehsi kgwitedtis llsgeedtkt rlleniiqhd
      361 rylhlckkln klqledekdd ntdvtkntlk plphlsvlqe egqkmelkvr afykgstive
      421 dpkntakdve qgsdlvpvlp lidshapkal rrrifldkln rilpdllral asnklpqyiy
      481 sneksalika lvntfslsat niifktaewt lvglviikml smidpqlkfl lsskqasmyi
      541 smilmsykld pnylnrlvme ltnikisnid nttnl
//
LOCUS       XP_034177110             575 aa            linear   INV 03-SEP-2025
DEFINITION  putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034177110
VERSION     XP_034177110.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177110.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="putative RNA polymerase II subunit B1 CTD
                     phosphatase RPAP2 isoform X2"
                     /calculated_mol_wt=65418
     Region          37..108
                     /region_name="RPAP2_Rtr1"
                     /note="Rtr1/RPAP2 family; pfam04181"
                     /db_xref="CDD:461212"
     CDS             1..575
                     /gene="LOC117602773"
                     /coded_by="XM_034321219.2:290..2017"
                     /db_xref="GeneID:117602773"
ORIGIN      
        1 mqlaiikkkq cdakaltive qllepkidsd wlldnlkhin kshmedviee rgitklcgyv
       61 lcsnalktii eqqyyistkk nkvydvtkrk nfcssrcyga snyllqqmlt splwlrdkee
      121 ipvfkvlpak deleksipgd eiqfndtivi lnsdnkdepa esntevdahd eenavknset
      181 vntyielnns empsvskees sknldefgdi kdvpidskgm idnvkqcmdn ndddkkmftl
      241 ensiilddsk iddtnnlsdt vsinkdadqs vkndqeknnv tiskqdtnis ndgaqkmgki
      301 qqnkpkrknv tkdkqssefy nlamniehsi kgwitedtis llsgeedtkt rlleniiqhd
      361 rylhlckkln klqledekdd ntdvtkntlk plphlsvlqe egqkmelkvr afykgstive
      421 dpkntakdve qgsdlvpvlp lidshapkal rrrifldkln rilpdllral asnklpqyiy
      481 sneksalika lvntfslsat niifktaewt lvglviikml smidpqlkfl lsskqasmyi
      541 smilmsykld pnylnrlvme ltnikisnid nttnl
//
LOCUS       XP_034177120             575 aa            linear   INV 03-SEP-2025
DEFINITION  putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034177120
VERSION     XP_034177120.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321229.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177120.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="putative RNA polymerase II subunit B1 CTD
                     phosphatase RPAP2 isoform X2"
                     /calculated_mol_wt=65418
     Region          37..108
                     /region_name="RPAP2_Rtr1"
                     /note="Rtr1/RPAP2 family; pfam04181"
                     /db_xref="CDD:461212"
     CDS             1..575
                     /gene="LOC117602773"
                     /coded_by="XM_034321229.2:267..1994"
                     /db_xref="GeneID:117602773"
ORIGIN      
        1 mqlaiikkkq cdakaltive qllepkidsd wlldnlkhin kshmedviee rgitklcgyv
       61 lcsnalktii eqqyyistkk nkvydvtkrk nfcssrcyga snyllqqmlt splwlrdkee
      121 ipvfkvlpak deleksipgd eiqfndtivi lnsdnkdepa esntevdahd eenavknset
      181 vntyielnns empsvskees sknldefgdi kdvpidskgm idnvkqcmdn ndddkkmftl
      241 ensiilddsk iddtnnlsdt vsinkdadqs vkndqeknnv tiskqdtnis ndgaqkmgki
      301 qqnkpkrknv tkdkqssefy nlamniehsi kgwitedtis llsgeedtkt rlleniiqhd
      361 rylhlckkln klqledekdd ntdvtkntlk plphlsvlqe egqkmelkvr afykgstive
      421 dpkntakdve qgsdlvpvlp lidshapkal rrrifldkln rilpdllral asnklpqyiy
      481 sneksalika lvntfslsat niifktaewt lvglviikml smidpqlkfl lsskqasmyi
      541 smilmsykld pnylnrlvme ltnikisnid nttnl
//
LOCUS       XP_034177130             575 aa            linear   INV 03-SEP-2025
DEFINITION  putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034177130
VERSION     XP_034177130.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321239.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177130.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="putative RNA polymerase II subunit B1 CTD
                     phosphatase RPAP2 isoform X2"
                     /calculated_mol_wt=65418
     Region          37..108
                     /region_name="RPAP2_Rtr1"
                     /note="Rtr1/RPAP2 family; pfam04181"
                     /db_xref="CDD:461212"
     CDS             1..575
                     /gene="LOC117602773"
                     /coded_by="XM_034321239.2:286..2013"
                     /db_xref="GeneID:117602773"
ORIGIN      
        1 mqlaiikkkq cdakaltive qllepkidsd wlldnlkhin kshmedviee rgitklcgyv
       61 lcsnalktii eqqyyistkk nkvydvtkrk nfcssrcyga snyllqqmlt splwlrdkee
      121 ipvfkvlpak deleksipgd eiqfndtivi lnsdnkdepa esntevdahd eenavknset
      181 vntyielnns empsvskees sknldefgdi kdvpidskgm idnvkqcmdn ndddkkmftl
      241 ensiilddsk iddtnnlsdt vsinkdadqs vkndqeknnv tiskqdtnis ndgaqkmgki
      301 qqnkpkrknv tkdkqssefy nlamniehsi kgwitedtis llsgeedtkt rlleniiqhd
      361 rylhlckkln klqledekdd ntdvtkntlk plphlsvlqe egqkmelkvr afykgstive
      421 dpkntakdve qgsdlvpvlp lidshapkal rrrifldkln rilpdllral asnklpqyiy
      481 sneksalika lvntfslsat niifktaewt lvglviikml smidpqlkfl lsskqasmyi
      541 smilmsykld pnylnrlvme ltnikisnid nttnl
//
LOCUS       XP_034177144             563 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 102A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177144
VERSION     XP_034177144.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321253.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..563
                     /product="coiled-coil domain-containing protein 102A
                     isoform X1"
                     /calculated_mol_wt=65475
     Region          <185..>509
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..563
                     /gene="LOC117602786"
                     /coded_by="XM_034321253.2:202..1893"
                     /db_xref="GeneID:117602786"
ORIGIN      
        1 maqstasgts srryirehdv nvpsssryvd sewetkealr qreleearar aaqmektmrw
       61 wsdctanwre kwskvrnern mareeakifr akleiavkda nsykhecqel elqndqlkke
      121 mekihmillk hagqfdqqif tvlesdpqlr ntlgidelik fynnveqser vntqkdlltc
      181 kssleesnvc lgghtilpdr dieeyvlqga vpkhavelyk espinsldkd iarlvtdass
      241 vednveknqs slqtstdesy aqkiltlqyk ldeatktist ereeknslhr gmeklkaevv
      301 qlrkqcgelv eskdeaikel lelkerfqfe lsdvqadiid qassregmnr rlcelraele
      361 klqaenaaew gkrerletek islerenkql rnelhdlqer ielrrsrpvs tsdsdtrqlq
      421 qeflvrnvti lkksleektt elshamrrse qyeaevkrvr arveelkkel asaqdevdaa
      481 ansvrklqra nedlleqles anvqlehfrn stdsysvdvg mgqaveaklc inddkltngr
      541 flhfnkfdif efiilsykyc frv
//
LOCUS       XP_034177153             545 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 102A isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177153
VERSION     XP_034177153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321262.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..545
                     /product="coiled-coil domain-containing protein 102A
                     isoform X2"
                     /calculated_mol_wt=63125
     Region          <185..>509
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..545
                     /gene="LOC117602786"
                     /coded_by="XM_034321262.2:202..1839"
                     /db_xref="GeneID:117602786"
ORIGIN      
        1 maqstasgts srryirehdv nvpsssryvd sewetkealr qreleearar aaqmektmrw
       61 wsdctanwre kwskvrnern mareeakifr akleiavkda nsykhecqel elqndqlkke
      121 mekihmillk hagqfdqqif tvlesdpqlr ntlgidelik fynnveqser vntqkdlltc
      181 kssleesnvc lgghtilpdr dieeyvlqga vpkhavelyk espinsldkd iarlvtdass
      241 vednveknqs slqtstdesy aqkiltlqyk ldeatktist ereeknslhr gmeklkaevv
      301 qlrkqcgelv eskdeaikel lelkerfqfe lsdvqadiid qassregmnr rlcelraele
      361 klqaenaaew gkrerletek islerenkql rnelhdlqer ielrrsrpvs tsdsdtrqlq
      421 qeflvrnvti lkksleektt elshamrrse qyeaevkrvr arveelkkel asaqdevdaa
      481 ansvrklqra nedlleqles anvqlehfrn stdsysvdvg mgqaveaklc inddkltney
      541 esqqa
//
LOCUS       XP_034177162             525 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 102A isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034177162
VERSION     XP_034177162.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321271.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="coiled-coil domain-containing protein 102A
                     isoform X3"
                     /calculated_mol_wt=61132
     Region          <152..>471
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..525
                     /gene="LOC117602786"
                     /coded_by="XM_034321271.2:202..1779"
                     /db_xref="GeneID:117602786"
ORIGIN      
        1 maqstasgts srryirehdv nvpsssryvd sewetkealr qreleearar aaqmektmrw
       61 wsdctanwre kwskvrnern mareeakifr akleiavkda nsykhecqel elqndqlkke
      121 mekihmillk hagqfdqqif tvlesdpqlr ntlgghtilp drdieeyvlq gavpkhavel
      181 ykespinsld kdiarlvtda ssvednvekn qsslqtstde syaqkiltlq ykldeatkti
      241 stereeknsl hrgmeklkae vvqlrkqcge lveskdeaik ellelkerfq felsdvqadi
      301 idqassregm nrrlcelrae leklqaenaa ewgkrerlet ekislerenk qlrnelhdlq
      361 erielrrsrp vstsdsdtrq lqqeflvrnv tilkksleek ttelshamrr seqyeaevkr
      421 vrarveelkk elasaqdevd aaansvrklq ranedlleql esanvqlehf rnstdsysvd
      481 vgmgqaveak lcinddkltn grflhfnkfd ifefiilsyk ycfrv
//
LOCUS       XP_034177187             528 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602807 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177187
VERSION     XP_034177187.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321296.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177187.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..528
                     /product="uncharacterized protein LOC117602807 isoform X1"
                     /calculated_mol_wt=59094
     Region          222..244
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          224..244
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..301
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          281..301
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(286,288,290,292..293,296..297,300,314,316,320..321,
                     324..325,328,342,344,346,348..349,352..353)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          294..317
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          307..329
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          309..329
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          337..353
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..528
                     /gene="LOC117602807"
                     /coded_by="XM_034321296.2:1467..3053"
                     /db_xref="GeneID:117602807"
ORIGIN      
        1 mpraflithr ryngveeefd gsgrdfsper gvrladysgn hptsdgasec gsetssecpe
       61 elynltklae vslaaaagtl ihpnnviyqq pasprcaqhs ekcgrmmtpr trqqdsqfqd
      121 htrtlegeqt lgertrlffe riesereilq nhserntvlg ihitprshhl qeerrgldis
      181 ensldktele spppsipsti sqsrrttsts tetsksedne dhecpdcgkk ystssnlarh
      241 rqthrslgdk karrcphcdk vyvsmpafsm hvrthnqgck chycgkcfsr pwllqghirt
      301 htgekpfkct icnkafadks nlrahiqths ntkphvcgrc gkafalksyl ykheesscmr
      361 ahhrssteki dgndpkttss stksctplps plnrlqttpc vtasptsviv prlglnreqr
      421 nsnsafsaii rhtrlpdgst splpskrscr ektpveeher ktpdniakdp eclsrmvirt
      481 svispnpehl srfnndsqns sgsfdfsdpt rssafsrptt mtlnlaia
//
LOCUS       XP_034177193             432 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602807 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034177193
VERSION     XP_034177193.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321302.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177193.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..432
                     /product="uncharacterized protein LOC117602807 isoform X2"
                     /calculated_mol_wt=48567
     Region          222..244
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          224..244
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          279..301
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          281..301
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(286,288,290,292..293,296..297,300,314,316,320..321,
                     324..325,328,342,344,346,348..349,352..353)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          294..317
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          307..329
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          309..329
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          337..353
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..432
                     /gene="LOC117602807"
                     /coded_by="XM_034321302.2:1467..2765"
                     /db_xref="GeneID:117602807"
ORIGIN      
        1 mpraflithr ryngveeefd gsgrdfsper gvrladysgn hptsdgasec gsetssecpe
       61 elynltklae vslaaaagtl ihpnnviyqq pasprcaqhs ekcgrmmtpr trqqdsqfqd
      121 htrtlegeqt lgertrlffe riesereilq nhserntvlg ihitprshhl qeerrgldis
      181 ensldktele spppsipsti sqsrrttsts tetsksedne dhecpdcgkk ystssnlarh
      241 rqthrslgdk karrcphcdk vyvsmpafsm hvrthnqgck chycgkcfsr pwllqghirt
      301 htgekpfkct icnkafadks nlrahiqths ntkphvcgrc gkafalksyl ykheesscmr
      361 ahhrssteki dgndpkttss stksctplps plnrlqttpc vtasptsviv prlglnqkrh
      421 lskntkerhl it
//
LOCUS       XP_034177202             426 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 35 member C2 [Osmia lignaria lignaria].
ACCESSION   XP_034177202
VERSION     XP_034177202.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177202.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="solute carrier family 35 member C2"
                     /calculated_mol_wt=48270
     Region          117..356
                     /region_name="TPT_S35C2"
                     /note="solute carrier family 35 member C2, member of the
                     triose-phosphate transporter family; cd21092"
                     /db_xref="CDD:411044"
     CDS             1..426
                     /gene="LOC117602819"
                     /coded_by="XM_034321311.2:395..1675"
                     /db_xref="GeneID:117602819"
ORIGIN      
        1 mtrshikyei akdaddtsdy flqpvqefpn tskkevspwk klckiltliv iyfvlsvglt
       61 fyvqwlynsy gfhfpltvvi chlsikflls slirciktcw krqqqlklsl hsilwmvmpv
      121 giasgldvgl snwaislitm slytmtkstt iifilgfali lklerkswsl icivvmisgg
      181 lfmftykstq faifgfvicl lasfssgvrw tmtqlimqks klgmkspidm myymqlwmll
      241 pvvpvmiwfe gshvyssfkn idwndvqsia mttiavlgsa iiafhmevme flvvtytssl
      301 tlsitgiike icililavew kgdqisglnf igllmclggi iihsiqkvls nrnkknenle
      361 lqvnsslsln lkneegidtn lplltqksts itnllnaefs seeddtmkhg enstqilsni
      421 lqrreq
//
LOCUS       XP_034188803             425 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188803
VERSION     XP_034188803.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332912.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="ecdysis triggering hormone receptor isoform X1"
                     /calculated_mol_wt=46853
     Region          50..340
                     /region_name="7tmA_ETH-R"
                     /note="ecdysis-triggering hormone receptors, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14997"
                     /db_xref="CDD:320128"
     Region          51..77
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          84..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Site            order(106,109..110,124..129,131..132,135,180,182..186,215,
                     218..220,222..224,226..227,281,284..285,287..288,291,
                     307..308,310..312,315,318..319)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320128"
     Region          124..154
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          167..188
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          215..244
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          261..291
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          308..333
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..425
                     /gene="ETHR"
                     /coded_by="XM_034332912.2:1987..3264"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mlstvssvsl efnssyyatv ignnsldgls viesenstkg lymlpayirt tsmvvciivm
       61 algivgnlmv plvvfrgkdm rnstniflvn lsvadlcvll ictptvlvev nsgpqvwplg
      121 ehmckavpfv eltvahasvl tilaisfery yaiceplrag yvctkaratf lcllawvaaa
      181 lctspillmv syevekntdg tyistcniva nsdwasgfiv ttilvffvip llilvilytv
      241 iarhlmtnpt isrgssnnll kyrkqvmlml gtvvlcfflc llpfraftlw ilvvppkmit
      301 dlgiegyftl lyfcrvmlyl nsainpilyn lmstkfregf lrlcglaptr rkkkktsgdk
      361 tgtyttgstn cssnhsdfwr rhssnksssv kvpssnssge kqiklplqta vvsgnivrkk
      421 qesyv
//
LOCUS       XP_034188991             425 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188991
VERSION     XP_034188991.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333100.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="ecdysis triggering hormone receptor isoform X1"
                     /calculated_mol_wt=46853
     Region          50..340
                     /region_name="7tmA_ETH-R"
                     /note="ecdysis-triggering hormone receptors, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14997"
                     /db_xref="CDD:320128"
     Region          51..77
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          84..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Site            order(106,109..110,124..129,131..132,135,180,182..186,215,
                     218..220,222..224,226..227,281,284..285,287..288,291,
                     307..308,310..312,315,318..319)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320128"
     Region          124..154
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          167..188
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          215..244
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          261..291
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          308..333
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..425
                     /gene="ETHR"
                     /coded_by="XM_034333100.2:1348..2625"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mlstvssvsl efnssyyatv ignnsldgls viesenstkg lymlpayirt tsmvvciivm
       61 algivgnlmv plvvfrgkdm rnstniflvn lsvadlcvll ictptvlvev nsgpqvwplg
      121 ehmckavpfv eltvahasvl tilaisfery yaiceplrag yvctkaratf lcllawvaaa
      181 lctspillmv syevekntdg tyistcniva nsdwasgfiv ttilvffvip llilvilytv
      241 iarhlmtnpt isrgssnnll kyrkqvmlml gtvvlcfflc llpfraftlw ilvvppkmit
      301 dlgiegyftl lyfcrvmlyl nsainpilyn lmstkfregf lrlcglaptr rkkkktsgdk
      361 tgtyttgstn cssnhsdfwr rhssnksssv kvpssnssge kqiklplqta vvsgnivrkk
      421 qesyv
//
LOCUS       XP_034189081             425 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189081
VERSION     XP_034189081.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333190.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="ecdysis triggering hormone receptor isoform X1"
                     /calculated_mol_wt=46853
     Region          50..340
                     /region_name="7tmA_ETH-R"
                     /note="ecdysis-triggering hormone receptors, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14997"
                     /db_xref="CDD:320128"
     Region          51..77
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          84..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Site            order(106,109..110,124..129,131..132,135,180,182..186,215,
                     218..220,222..224,226..227,281,284..285,287..288,291,
                     307..308,310..312,315,318..319)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320128"
     Region          124..154
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          167..188
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          215..244
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          261..291
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          308..333
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..425
                     /gene="ETHR"
                     /coded_by="XM_034333190.2:1345..2622"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mlstvssvsl efnssyyatv ignnsldgls viesenstkg lymlpayirt tsmvvciivm
       61 algivgnlmv plvvfrgkdm rnstniflvn lsvadlcvll ictptvlvev nsgpqvwplg
      121 ehmckavpfv eltvahasvl tilaisfery yaiceplrag yvctkaratf lcllawvaaa
      181 lctspillmv syevekntdg tyistcniva nsdwasgfiv ttilvffvip llilvilytv
      241 iarhlmtnpt isrgssnnll kyrkqvmlml gtvvlcfflc llpfraftlw ilvvppkmit
      301 dlgiegyftl lyfcrvmlyl nsainpilyn lmstkfregf lrlcglaptr rkkkktsgdk
      361 tgtyttgstn cssnhsdfwr rhssnksssv kvpssnssge kqiklplqta vvsgnivrkk
      421 qesyv
//
LOCUS       XP_034189269             410 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189269
VERSION     XP_034189269.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333378.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="ecdysis triggering hormone receptor isoform X2"
                     /calculated_mol_wt=45486
     Region          35..325
                     /region_name="7tmA_ETH-R"
                     /note="ecdysis-triggering hormone receptors, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14997"
                     /db_xref="CDD:320128"
     Region          36..62
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          69..94
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Site            order(91,94..95,109..114,116..117,120,165,167..171,200,
                     203..205,207..209,211..212,266,269..270,272..273,276,
                     292..293,295..297,300,303..304)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320128"
     Region          109..139
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          152..173
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          200..229
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          246..276
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          293..318
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..410
                     /gene="ETHR"
                     /coded_by="XM_034333378.2:565..1797"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mintvllkre qqestviese nstkglymlp ayirttsmvv ciivmalgiv gnlmvplvvf
       61 rgkdmrnstn iflvnlsvad lcvllictpt vlvevnsgpq vwplgehmck avpfveltva
      121 hasvltilai sferyyaice plragyvctk aratflclla wvaaalctsp illmvsyeve
      181 kntdgtyist cnivansdwa sgfivttilv ffvipllilv ilytviarhl mtnptisrgs
      241 snnllkyrkq vmlmlgtvvl cfflcllpfr aftlwilvvp pkmitdlgie gyftllyfcr
      301 vmlylnsain pilynlmstk fregflrlcg laptrrkkkk tsgdktgtyt tgstncssnh
      361 sdfwrrhssn ksssvkvpss nssgekqikl plqtavvsgn ivrkkqesyv
//
LOCUS       XP_034189447             406 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189447
VERSION     XP_034189447.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333556.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="ecdysis triggering hormone receptor isoform X3"
                     /calculated_mol_wt=44958
     Region          50..340
                     /region_name="7tmA_ETH-R"
                     /note="ecdysis-triggering hormone receptors, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14997"
                     /db_xref="CDD:320128"
     Region          51..77
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          84..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Site            order(106,109..110,124..129,131..132,135,180,182..186,215,
                     218..220,222..224,226..227,281,284..285,287..288,291,
                     307..308,310..312,315,318..319)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320128"
     Region          124..154
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          167..188
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          215..244
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          261..291
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          308..333
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..406
                     /gene="ETHR"
                     /coded_by="XM_034333556.2:1987..3207"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mlstvssvsl efnssyyatv ignnsldgls viesenstkg lymlpayirt tsmvvciivm
       61 algivgnlmv plvvfrgkdm rnstniflvn lsvadlcvll ictptvlvev nsgpqvwplg
      121 ehmckavpfv eltvahasvl tilaisfery yaiceplrag yvctkaratf lcllawvaaa
      181 lctspillmv syevekntdg tyistcniva nsdwasgfiv ttilvffvip llilvilytv
      241 iarhlmtnpt isrgssnnll kyrkqvmlml gtvvlcfflc llpfraftlw ilvvppkmit
      301 dlgiegyftl lyfcrvmlyl nsainpilyn lmstkfregf lrlcglaptr rkkkktsgdk
      361 tgtyttgstn cssnhsdfwr rhssnksssv krsevyshse nvndie
//
LOCUS       XP_076546242             383 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546242
VERSION     XP_076546242.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690127.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="ecdysis triggering hormone receptor isoform X4"
                     /calculated_mol_wt=42393
     Region          8..298
                     /region_name="7tmA_ETH-R"
                     /note="ecdysis-triggering hormone receptors, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14997"
                     /db_xref="CDD:320128"
     Region          9..35
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          42..67
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Site            order(64,67..68,82..87,89..90,93,138,140..144,173,
                     176..178,180..182,184..185,239,242..243,245..246,249,
                     265..266,268..270,273,276..277)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320128"
     Region          82..112
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          125..146
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          173..202
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          219..249
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          266..291
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..383
                     /gene="ETHR"
                     /coded_by="XM_076690127.1:347..1498"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mlpayirtts mvvciivmal givgnlmvpl vvfrgkdmrn stniflvnls vadlcvllic
       61 tptvlvevns gpqvwplgeh mckavpfvel tvahasvlti laisferyya iceplragyv
      121 ctkaratflc llawvaaalc tspillmvsy evekntdgty istcnivans dwasgfivtt
      181 ilvffvipll ilvilytvia rhlmtnptis rgssnnllky rkqvmlmlgt vvlcfflcll
      241 pfraftlwil vvppkmitdl giegyftlly fcrvmlylns ainpilynlm stkfregflr
      301 lcglaptrrk kkktsgdktg tyttgstncs snhsdfwrrh ssnksssvkv pssnssgekq
      361 iklplqtavv sgnivrkkqe syv
//
LOCUS       XP_076546243             383 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546243
VERSION     XP_076546243.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690128.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="ecdysis triggering hormone receptor isoform X4"
                     /calculated_mol_wt=42393
     Region          8..298
                     /region_name="7tmA_ETH-R"
                     /note="ecdysis-triggering hormone receptors, member of the
                     class A family of seven-transmembrane G protein-coupled
                     receptors; cd14997"
                     /db_xref="CDD:320128"
     Region          9..35
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          42..67
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Site            order(64,67..68,82..87,89..90,93,138,140..144,173,
                     176..178,180..182,184..185,239,242..243,245..246,249,
                     265..266,268..270,273,276..277)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320128"
     Region          82..112
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          125..146
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          173..202
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          219..249
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          266..291
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..383
                     /gene="ETHR"
                     /coded_by="XM_076690128.1:343..1494"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mlpayirtts mvvciivmal givgnlmvpl vvfrgkdmrn stniflvnls vadlcvllic
       61 tptvlvevns gpqvwplgeh mckavpfvel tvahasvlti laisferyya iceplragyv
      121 ctkaratflc llawvaaalc tspillmvsy evekntdgty istcnivans dwasgfivtt
      181 ilvffvipll ilvilytvia rhlmtnptis rgssnnllky rkqvmlmlgt vvlcfflcll
      241 pfraftlwil vvppkmitdl giegyftlly fcrvmlylns ainpilynlm stkfregflr
      301 lcglaptrrk kkktsgdktg tyttgstncs snhsdfwrrh ssnksssvkv pssnssgekq
      361 iklplqtavv sgnivrkkqe syv
//
LOCUS       XP_034189629             376 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189629
VERSION     XP_034189629.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333738.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ecdysis triggering hormone receptor isoform X5"
                     /calculated_mol_wt=42248
     Region          50..345
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          52..76
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320128"
     Region          85..107
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Region          125..147
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          170..186
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          223..246
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          272..294
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          313..338
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..376
                     /gene="ETHR"
                     /coded_by="XM_034333738.2:1975..3105"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mlstvssvsl efnssyyatv ignnsldgls viesenstkg lymlpayirt tsmvvciivm
       61 algivgnlmv plvvfrgkdm rnstniflvn lsvadlcvll ictptvlvev nsgpqvwplg
      121 ehmckavpfv eltvahasvl tilaisfery yaiceplrag yvctkaratf lcllawvaaa
      181 lctspiiwvs qyqkmrlkes kddqdqrnfi pvcltsvdtt vttvfflflv lffyllplli
      241 lmilyaliir nlvpdssnts dtyharakrh vitmlmsvvl sfflcltpyr ilifyiivap
      301 aeqiaaidrd tffaflnfsr imfylhsavd pilynlmssk frrgflqlcr crksnrlkss
      361 ftgtrktdst eqenfv
//
LOCUS       XP_076546244             346 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546244
VERSION     XP_076546244.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690129.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="ecdysis triggering hormone receptor isoform X6"
                     /calculated_mol_wt=38389
     Region          1..261
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          6..28
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Region          46..68
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          91..107
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          137..160
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          188..210
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          229..254
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..346
                     /gene="ETHR"
                     /coded_by="XM_076690129.1:295..1335"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mrnstniflv nlsvadlcvl lictptvlve vnsgpqvwpl gehmckavpf veltvahasv
       61 ltilaisfer yyaiceplra gyvctkarat flcllawvaa alctspillm vsyevekntd
      121 gtyistcniv ansdwasgfi vttilvffvi pllilvilyt viarhlmtnp tisrgssnnl
      181 lkyrkqvmlm lgtvvlcffl cllpfraftl wilvvppkmi tdlgiegyft llyfcrvmly
      241 lnsainpily nlmstkfreg flrlcglapt rrkkkktsgd ktgtyttgst ncssnhsdfw
      301 rrhssnksss vkvpssnssg ekqiklplqt avvsgnivrk kqesyv
//
LOCUS       XP_076546245             346 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysis triggering hormone receptor isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546245
VERSION     XP_076546245.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690130.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="ecdysis triggering hormone receptor isoform X6"
                     /calculated_mol_wt=38389
     Region          1..261
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          6..28
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320128"
     Region          46..68
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320128"
     Region          91..107
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320128"
     Region          137..160
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320128"
     Region          188..210
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320128"
     Region          229..254
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320128"
     CDS             1..346
                     /gene="ETHR"
                     /coded_by="XM_076690130.1:312..1352"
                     /db_xref="GeneID:117608176"
ORIGIN      
        1 mrnstniflv nlsvadlcvl lictptvlve vnsgpqvwpl gehmckavpf veltvahasv
       61 ltilaisfer yyaiceplra gyvctkarat flcllawvaa alctspillm vsyevekntd
      121 gtyistcniv ansdwasgfi vttilvffvi pllilvilyt viarhlmtnp tisrgssnnl
      181 lkyrkqvmlm lgtvvlcffl cllpfraftl wilvvppkmi tdlgiegyft llyfcrvmly
      241 lnsainpily nlmstkfreg flrlcglapt rrkkkktsgd ktgtyttgst ncssnhsdfw
      301 rrhssnksss vkvpssnssg ekqiklplqt avvsgnivrk kqesyv
//
LOCUS       XP_076546246             389 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase interacting tpr containing protein of 47kD [Osmia
            lignaria lignaria].
ACCESSION   XP_076546246
VERSION     XP_076546246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690131.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="DNA polymerase interacting tpr containing
                     protein of 47kD"
                     /calculated_mol_wt=45637
     Region          <78..219
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          94..122
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(95,98..99,102..103,105,133,136..137,140..141,
                     143..144,167,170..171,174..175,178)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          131..161
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          166..194
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          239..387
                     /region_name="CTWD_Cns1"
                     /note="C-terminal wheel domain found in Saccharomyces
                     cerevisiae Hsp70/Hsp90 co-chaperone Cns1 and similar
                     proteins; cd21380"
                     /db_xref="CDD:439174"
     CDS             1..389
                     /gene="Dpit47"
                     /coded_by="XM_076690131.1:146..1315"
                     /db_xref="GeneID:117604745"
ORIGIN      
        1 meekangkqs ktwtdeerla laskldaeld eyisnlekks ytegwpedrw eeemekhpff
       61 mkkapepgee lsplmeglqq lkyganentp eelannyked gnfnykykky rlailsyteg
      121 irtkckdndl maqlynnraa ahfmlknyrs slndckhalk lkpnytkaln raatccfhik
      181 vydqcidlcd qlldqsptdk eilnlksqai aarerlkrdk rkqerlekkl dkeeeellsi
      241 ikskgislel iegrknpdlk dlepqvpqia qsrvhldaen kliwpvmily pethqtdfiq
      301 nfhqdtllie qleqlfnepp ewdterrytl qninvyfenk nkcsihlvna hhslekilqd
      361 erfivrggtp vflifvksse aekrflany
//
LOCUS       XP_076546247             389 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase interacting tpr containing protein of 47kD [Osmia
            lignaria lignaria].
ACCESSION   XP_076546247
VERSION     XP_076546247.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690132.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="DNA polymerase interacting tpr containing
                     protein of 47kD"
                     /calculated_mol_wt=45637
     Region          <78..219
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          94..122
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(95,98..99,102..103,105,133,136..137,140..141,
                     143..144,167,170..171,174..175,178)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          131..161
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          166..194
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          239..387
                     /region_name="CTWD_Cns1"
                     /note="C-terminal wheel domain found in Saccharomyces
                     cerevisiae Hsp70/Hsp90 co-chaperone Cns1 and similar
                     proteins; cd21380"
                     /db_xref="CDD:439174"
     CDS             1..389
                     /gene="Dpit47"
                     /coded_by="XM_076690132.1:146..1315"
                     /db_xref="GeneID:117604745"
ORIGIN      
        1 meekangkqs ktwtdeerla laskldaeld eyisnlekks ytegwpedrw eeemekhpff
       61 mkkapepgee lsplmeglqq lkyganentp eelannyked gnfnykykky rlailsyteg
      121 irtkckdndl maqlynnraa ahfmlknyrs slndckhalk lkpnytkaln raatccfhik
      181 vydqcidlcd qlldqsptdk eilnlksqai aarerlkrdk rkqerlekkl dkeeeellsi
      241 ikskgislel iegrknpdlk dlepqvpqia qsrvhldaen kliwpvmily pethqtdfiq
      301 nfhqdtllie qleqlfnepp ewdterrytl qninvyfenk nkcsihlvna hhslekilqd
      361 erfivrggtp vflifvksse aekrflany
//
LOCUS       XP_076546248             389 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase interacting tpr containing protein of 47kD [Osmia
            lignaria lignaria].
ACCESSION   XP_076546248
VERSION     XP_076546248.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690133.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="DNA polymerase interacting tpr containing
                     protein of 47kD"
                     /calculated_mol_wt=45637
     Region          <78..219
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          94..122
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(95,98..99,102..103,105,133,136..137,140..141,
                     143..144,167,170..171,174..175,178)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          131..161
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          166..194
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          239..387
                     /region_name="CTWD_Cns1"
                     /note="C-terminal wheel domain found in Saccharomyces
                     cerevisiae Hsp70/Hsp90 co-chaperone Cns1 and similar
                     proteins; cd21380"
                     /db_xref="CDD:439174"
     CDS             1..389
                     /gene="Dpit47"
                     /coded_by="XM_076690133.1:146..1315"
                     /db_xref="GeneID:117604745"
ORIGIN      
        1 meekangkqs ktwtdeerla laskldaeld eyisnlekks ytegwpedrw eeemekhpff
       61 mkkapepgee lsplmeglqq lkyganentp eelannyked gnfnykykky rlailsyteg
      121 irtkckdndl maqlynnraa ahfmlknyrs slndckhalk lkpnytkaln raatccfhik
      181 vydqcidlcd qlldqsptdk eilnlksqai aarerlkrdk rkqerlekkl dkeeeellsi
      241 ikskgislel iegrknpdlk dlepqvpqia qsrvhldaen kliwpvmily pethqtdfiq
      301 nfhqdtllie qleqlfnepp ewdterrytl qninvyfenk nkcsihlvna hhslekilqd
      361 erfivrggtp vflifvksse aekrflany
//
LOCUS       XP_076546249             389 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase interacting tpr containing protein of 47kD [Osmia
            lignaria lignaria].
ACCESSION   XP_076546249
VERSION     XP_076546249.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690134.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="DNA polymerase interacting tpr containing
                     protein of 47kD"
                     /calculated_mol_wt=45637
     Region          <78..219
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          94..122
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(95,98..99,102..103,105,133,136..137,140..141,
                     143..144,167,170..171,174..175,178)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          131..161
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          166..194
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          239..387
                     /region_name="CTWD_Cns1"
                     /note="C-terminal wheel domain found in Saccharomyces
                     cerevisiae Hsp70/Hsp90 co-chaperone Cns1 and similar
                     proteins; cd21380"
                     /db_xref="CDD:439174"
     CDS             1..389
                     /gene="Dpit47"
                     /coded_by="XM_076690134.1:146..1315"
                     /db_xref="GeneID:117604745"
ORIGIN      
        1 meekangkqs ktwtdeerla laskldaeld eyisnlekks ytegwpedrw eeemekhpff
       61 mkkapepgee lsplmeglqq lkyganentp eelannyked gnfnykykky rlailsyteg
      121 irtkckdndl maqlynnraa ahfmlknyrs slndckhalk lkpnytkaln raatccfhik
      181 vydqcidlcd qlldqsptdk eilnlksqai aarerlkrdk rkqerlekkl dkeeeellsi
      241 ikskgislel iegrknpdlk dlepqvpqia qsrvhldaen kliwpvmily pethqtdfiq
      301 nfhqdtllie qleqlfnepp ewdterrytl qninvyfenk nkcsihlvna hhslekilqd
      361 erfivrggtp vflifvksse aekrflany
//
LOCUS       XP_076546250             389 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase interacting tpr containing protein of 47kD [Osmia
            lignaria lignaria].
ACCESSION   XP_076546250
VERSION     XP_076546250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690135.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="DNA polymerase interacting tpr containing
                     protein of 47kD"
                     /calculated_mol_wt=45637
     Region          <78..219
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          94..122
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(95,98..99,102..103,105,133,136..137,140..141,
                     143..144,167,170..171,174..175,178)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          131..161
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          166..194
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          239..387
                     /region_name="CTWD_Cns1"
                     /note="C-terminal wheel domain found in Saccharomyces
                     cerevisiae Hsp70/Hsp90 co-chaperone Cns1 and similar
                     proteins; cd21380"
                     /db_xref="CDD:439174"
     CDS             1..389
                     /gene="Dpit47"
                     /coded_by="XM_076690135.1:146..1315"
                     /db_xref="GeneID:117604745"
ORIGIN      
        1 meekangkqs ktwtdeerla laskldaeld eyisnlekks ytegwpedrw eeemekhpff
       61 mkkapepgee lsplmeglqq lkyganentp eelannyked gnfnykykky rlailsyteg
      121 irtkckdndl maqlynnraa ahfmlknyrs slndckhalk lkpnytkaln raatccfhik
      181 vydqcidlcd qlldqsptdk eilnlksqai aarerlkrdk rkqerlekkl dkeeeellsi
      241 ikskgislel iegrknpdlk dlepqvpqia qsrvhldaen kliwpvmily pethqtdfiq
      301 nfhqdtllie qleqlfnepp ewdterrytl qninvyfenk nkcsihlvna hhslekilqd
      361 erfivrggtp vflifvksse aekrflany
//
LOCUS       XP_034177217             382 aa            linear   INV 03-SEP-2025
DEFINITION  thyrotropin-releasing hormone receptor [Osmia lignaria lignaria].
ACCESSION   XP_034177217
VERSION     XP_034177217.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321326.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177217.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..382
                     /product="thyrotropin-releasing hormone receptor"
                     /calculated_mol_wt=43575
     Region          21..322
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          21..47
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          54..79
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(76,79..80,91..96,98..99,102,147,149..153,182,
                     185..187,189..191,193..194,262,265..266,268..269,272,
                     289..290,292..294,297,300..301)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          91..121
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          133..153
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          182..207
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410630"
     Region          234..273
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          290..315
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..382
                     /gene="LOC117602828"
                     /coded_by="XM_034321326.2:1285..2433"
                     /db_xref="GeneID:117602828"
ORIGIN      
        1 mahtqrdlve fnltenvlrs vqlyytpmlv yfgslgncls vivffgtklc qysssiylga
       61 laisdtgflv svfvvwlnlv evglfnqqgf cqffiylttl csfmsvwlvv aftierfiav
      121 qyplyrqsmc tvarakaavt ilttlgvvlc spvlwfsapr lqygkngnvt echlaegles
      181 waivynvidt vltfaipftv iiilnvliar aiyrhikirk sltnepsimk ekqpqaqsfr
      241 nnlaqtkitk mllvvsstfl cfnlpayvlr iyaflhfqre snlsqvrsle laqqvcnllf
      301 ntnfginfil ycatgqnfrt aircmflkrf rrkntavmqv snhrmqnvsn yvrssttttv
      361 qrhpsgftep wqefhelnvi se
//
LOCUS       XP_034177174             547 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602802 [Osmia lignaria lignaria].
ACCESSION   XP_034177174
VERSION     XP_034177174.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321283.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034177174.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..547
                     /product="uncharacterized protein LOC117602802"
                     /calculated_mol_wt=60425
     Region          52..>213
                     /region_name="ANF_receptor"
                     /note="Receptor family ligand binding region; pfam01094"
                     /db_xref="CDD:460062"
     CDS             1..547
                     /gene="LOC117602802"
                     /coded_by="XM_034321283.2:82..1725"
                     /db_xref="GeneID:117602802"
ORIGIN      
        1 mdhtrwflvl lgiicactcg vktecgnhaq islltsihdd pscrklsarg vllyeaakll
       61 aelhnnksag ydieltvvdt cgsiagalka amkalvgadt nclqpphylg iigpdtatna
      121 eavhkitsvl kvphivrras nspylhhlak esdsylvqgt mkvieelkwk sftlvvnade
      181 dgeddaqnia kkltmaaiqk glcvlvydgd qddltthivh igtpedgffs kpinstvliv
      241 segrleghln hlnstnsill ledsrnefag letrvekskw wsnkdngrfd seelrdvrwl
      301 edaikvyaka ldtlckkkkc knqvnpvdwn nvltnvladq akesqsvpss lelsmkvkaa
      361 elqtlgsivv nknkvklhwg nedddddddk dgddddddde ddddddddge mpsmfkklit
      421 kekgtktgca ttpkqikllh ddddddddea trvlvsemdd sdwwtvvgtv sgvgvamfvv
      481 gilavyvvyt nirgpvppkn arierdtslr rvgsdretpv raqrqprtlq rmdsnrsirs
      541 tiseksv
//
LOCUS       XP_034177230             352 aa            linear   INV 03-SEP-2025
DEFINITION  DENN domain-containing protein 10 [Osmia lignaria lignaria].
ACCESSION   XP_034177230
VERSION     XP_034177230.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="DENN domain-containing protein 10"
                     /calculated_mol_wt=40389
     Region          151..259
                     /region_name="SPA"
                     /note="Stabilization of polarity axis; cl40631"
                     /db_xref="CDD:454797"
     CDS             1..352
                     /gene="LOC117602837"
                     /coded_by="XM_034321339.2:192..1250"
                     /db_xref="GeneID:117602837"
ORIGIN      
        1 mapltdllsc siiekdsngd ilwtwsyprv aesqkavvar kcnlelehns sqvfvfsrhk
       61 hswfyihcse vfdsdklpkv kqfalvlfak dfnprkyevl srvlskmyck tgkpteilql
      121 ylsvftkgsc stqengtfvs ddfnshrftm ntniremikt feletiliyt alllkkriiv
      181 yhhsleqllk wigifpelmk hrkvtdnlfp wvdliddela elkghshyia gcrnvfissr
      241 telfdllvni pareitvash akesltmtkt hkeialfmvq lsenqsytev qiiseindkt
      301 qdllnqlksl alvqgpdgrk mvsaqtlkek nlppavenfl inlavaenlf ll
//
LOCUS       XP_034189847             346 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608573 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189847
VERSION     XP_034189847.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333956.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189847.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="uncharacterized protein LOC117608573 isoform X1"
                     /calculated_mol_wt=40323
     Region          153..315
                     /region_name="Methyltransf_21"
                     /note="Methyltransferase FkbM domain; pfam05050"
                     /db_xref="CDD:428282"
     CDS             1..346
                     /gene="LOC117608573"
                     /coded_by="XM_034333956.2:1..1041"
                     /db_xref="GeneID:117608573"
ORIGIN      
        1 mcnfhscyec iqtyhlgccw rrkwyfimgf vlggsimlll lrestynpdi sqnsiepnin
       61 mdgelrnvyk evtnrwynlt dddkwynsri fsksdskswk rymteirqnw ilwkhnsnkp
      121 ynlehpeied psmgqasvir kifqdkkngf fvecgaydge trsntlvler fldwtgllve
      181 adsinfskmi lknrksylsp icfglqpypt vntflmadnv grlhdpnadn tmpnskdvay
      241 sgvhikvqcf pfahlmgaln vttvnyfsld ieghelevlk tipfdeinie tlsveyahva
      301 sgkkklidfm eskgyyvhsf lvrsdrland iiftkynydt fnstwn
//
LOCUS       XP_034189935             344 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608573 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189935
VERSION     XP_034189935.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334044.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189935.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..344
                     /product="uncharacterized protein LOC117608573 isoform X2"
                     /calculated_mol_wt=40023
     Region          153..315
                     /region_name="Methyltransf_21"
                     /note="Methyltransferase FkbM domain; pfam05050"
                     /db_xref="CDD:428282"
     CDS             1..344
                     /gene="LOC117608573"
                     /coded_by="XM_034334044.2:1..1035"
                     /db_xref="GeneID:117608573"
ORIGIN      
        1 mcnfhscyec iqtyhlgccw rrkwyfimgf vlggsimlll lrestynpdi sqnsiepnin
       61 mdgelrnvyk evtnrwynlt dddkwynsri fsksdskswk rymteirqnw ilwkhnsnkp
      121 ynlehpeied psmgqasvir kifqdkkngf fvecgaydge trsntlvler fldwtgllve
      181 adsinfskmi lknrksylsp icfglqpypt vntflmadnv grlhdpnadn tmpnskdvay
      241 sgvhikvqcf pfahlmgaln vttvnyfsld ieghelevlk tipfdeinie tlsveyahva
      301 sgkkklidfm eskgyyvhsf lvrsdrland iiftkynydt fnst
//
LOCUS       XP_034190022             336 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608573 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190022
VERSION     XP_034190022.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190022.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="uncharacterized protein LOC117608573 isoform X3"
                     /calculated_mol_wt=39060
     Region          143..305
                     /region_name="Methyltransf_21"
                     /note="Methyltransferase FkbM domain; pfam05050"
                     /db_xref="CDD:428282"
     CDS             1..336
                     /gene="LOC117608573"
                     /coded_by="XM_034334131.2:1..1011"
                     /db_xref="GeneID:117608573"
ORIGIN      
        1 mcnfhscyec iqtyhlgccw rrkwyfimgf vlggsimlll lrestynpdi sqnsiepnin
       61 mdgelrnvyk eddkwynsri fsksdskswk rymteirqnw ilwkhnsnkp ynlehpeied
      121 psmgqasvir kifqdkkngf fvecgaydge trsntlvler fldwtgllve adsinfskmi
      181 lknrksylsp icfglqpypt vntflmadnv grlhdpnadn tmpnskdvay sgvhikvqcf
      241 pfahlmgaln vttvnyfsld ieghelevlk tipfdeinie tlsveyahva sgkkklidfm
      301 eskgyyvhsf lvrsdrland iiftkynydt fnstwn
//
LOCUS       XP_034177244             300 aa            linear   INV 03-SEP-2025
DEFINITION  ADP/ATP translocase [Osmia lignaria lignaria].
ACCESSION   XP_034177244
VERSION     XP_034177244.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321353.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="ADP/ATP translocase"
                     /calculated_mol_wt=32745
     Region          10..300
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..300
                     /gene="Ant"
                     /coded_by="XM_034321353.2:107..1009"
                     /db_xref="GeneID:117602842"
ORIGIN      
        1 msgladpvaf lkdfvaggva aaisktavap iervklllqv qhiskqisee qrykgmvdcf
       61 vripkeqgfl sywrgnmanv iryfptqaln fafkdkykqv flggvdkntq fmryfvgnla
      121 sggaagatsl cfvypldfar trlaadvgka gaerefsglg ncltkifkad gigglyrgfg
      181 vsvqgiiiyr aayfgfydta rgmlpdpkkt pflvswgiaq avttvagivs ypfdtvrrrm
      241 mmqsgrakae ilykstlhcw atiykseggn affkgafsnv lrgtggalvl vlydeiknll
//
LOCUS       XP_034177257             273 aa            linear   INV 03-SEP-2025
DEFINITION  armadillo repeat-containing protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034177257
VERSION     XP_034177257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="armadillo repeat-containing protein 1"
                     /calculated_mol_wt=31225
     CDS             1..273
                     /gene="LOC117602848"
                     /coded_by="XM_034321366.2:150..971"
                     /db_xref="GeneID:117602848"
ORIGIN      
        1 mdvdesidpk elkvtletyk klandftnhd tilkdktvls yvayilevpd aeivnlgldi
       61 lelfvknldn yvhitstfgv realdavink ynleepkist raqslkndie rmkppiynlh
      121 srcrrviepk klkthvivlh vqgllpetra eleailirin glvslvvdve hqrvtmrtls
      181 yvtakqiaea iqknsenmea rlvtrnklnq eylvklihtg nnadtddmpe ylpeedeqed
      241 ekegvvslft glrqsassly kstaeflhns fyw
//
LOCUS       XP_034177264             273 aa            linear   INV 03-SEP-2025
DEFINITION  armadillo repeat-containing protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034177264
VERSION     XP_034177264.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321373.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="armadillo repeat-containing protein 1"
                     /calculated_mol_wt=31225
     CDS             1..273
                     /gene="LOC117602848"
                     /coded_by="XM_034321373.2:148..969"
                     /db_xref="GeneID:117602848"
ORIGIN      
        1 mdvdesidpk elkvtletyk klandftnhd tilkdktvls yvayilevpd aeivnlgldi
       61 lelfvknldn yvhitstfgv realdavink ynleepkist raqslkndie rmkppiynlh
      121 srcrrviepk klkthvivlh vqgllpetra eleailirin glvslvvdve hqrvtmrtls
      181 yvtakqiaea iqknsenmea rlvtrnklnq eylvklihtg nnadtddmpe ylpeedeqed
      241 ekegvvslft glrqsassly kstaeflhns fyw
//
LOCUS       XP_034177271             273 aa            linear   INV 03-SEP-2025
DEFINITION  armadillo repeat-containing protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034177271
VERSION     XP_034177271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321380.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="armadillo repeat-containing protein 1"
                     /calculated_mol_wt=31225
     CDS             1..273
                     /gene="LOC117602848"
                     /coded_by="XM_034321380.2:119..940"
                     /db_xref="GeneID:117602848"
ORIGIN      
        1 mdvdesidpk elkvtletyk klandftnhd tilkdktvls yvayilevpd aeivnlgldi
       61 lelfvknldn yvhitstfgv realdavink ynleepkist raqslkndie rmkppiynlh
      121 srcrrviepk klkthvivlh vqgllpetra eleailirin glvslvvdve hqrvtmrtls
      181 yvtakqiaea iqknsenmea rlvtrnklnq eylvklihtg nnadtddmpe ylpeedeqed
      241 ekegvvslft glrqsassly kstaeflhns fyw
//
LOCUS       XP_076546251             232 aa            linear   INV 03-SEP-2025
DEFINITION  SOSS complex subunit B homolog [Osmia lignaria lignaria].
ACCESSION   XP_076546251
VERSION     XP_076546251.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690136.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..232
                     /product="SOSS complex subunit B homolog"
                     /calculated_mol_wt=24439
     Region          17..94
                     /region_name="SoSSB_OBF"
                     /note="A subfamily of OB folds similar to the OB fold of
                     the crenarchaeote Sulfolobus solfataricus single-stranded
                     (ss) DNA-binding protein (SSoSSB). SSoSSB has a single OB
                     fold, and it physically and functionally interacts with
                     RNA polymerase. In vitro, SSoSSB...; cd04491"
                     /db_xref="CDD:239937"
     Site            order(17,66,68,70,92)
                     /site_type="active"
                     /note="generic binding surface II [active]"
                     /db_xref="CDD:239937"
     Site            order(22..24,39..42,44,50..52,54,65,73..75,82..84)
                     /site_type="active"
                     /note="generic binding surface I [active]"
                     /db_xref="CDD:239937"
     CDS             1..232
                     /gene="LOC143305665"
                     /coded_by="XM_076690136.1:1..699"
                     /db_xref="GeneID:143305665"
ORIGIN      
        1 meyvlikdir pgqkninvvf ivlevghpti tkenrevrtf kvadstacmn vsiwdepgql
       61 lvpgdivrlt kgyasvwrqc ltlysgkngd iqkigefcmv ineqlnmsep npalaqqmin
      121 qsgsgppgsn innnitnngn annisgqsgr qplaiqsnst tptsgapsss attksgnnnn
      181 gnsggnssss tsglpggsar ysgdtttktt vttktnsrgr ggyrnggrsd rr
//
LOCUS       XP_034177297             140 aa            linear   INV 03-SEP-2025
DEFINITION  vesicle transport protein GOT1B [Osmia lignaria lignaria].
ACCESSION   XP_034177297
VERSION     XP_034177297.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321406.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="vesicle transport protein GOT1B"
                     /calculated_mol_wt=15090
     Region          4..120
                     /region_name="Got1"
                     /note="Got1/Sft2-like family; cl02130"
                     /db_xref="CDD:470475"
     CDS             1..140
                     /gene="LOC117602871"
                     /coded_by="XM_034321406.2:232..654"
                     /db_xref="GeneID:117602871"
ORIGIN      
        1 mfeitdtqki gvglagfgis flflgvlllf dkgllaigni lfisglacvi gprrtlnfff
       61 qkhkikasis flggiivvlm gwpivgmile tygfvllfsg flpvainflr rvpllgtiln
      121 mpglsrildm iagdsnrttv
//
LOCUS       XP_034177305             140 aa            linear   INV 03-SEP-2025
DEFINITION  vesicle transport protein GOT1B [Osmia lignaria lignaria].
ACCESSION   XP_034177305
VERSION     XP_034177305.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321414.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="vesicle transport protein GOT1B"
                     /calculated_mol_wt=15090
     Region          4..120
                     /region_name="Got1"
                     /note="Got1/Sft2-like family; cl02130"
                     /db_xref="CDD:470475"
     CDS             1..140
                     /gene="LOC117602871"
                     /coded_by="XM_034321414.2:249..671"
                     /db_xref="GeneID:117602871"
ORIGIN      
        1 mfeitdtqki gvglagfgis flflgvlllf dkgllaigni lfisglacvi gprrtlnfff
       61 qkhkikasis flggiivvlm gwpivgmile tygfvllfsg flpvainflr rvpllgtiln
      121 mpglsrildm iagdsnrttv
//
LOCUS       XP_034177312             140 aa            linear   INV 03-SEP-2025
DEFINITION  vesicle transport protein GOT1B [Osmia lignaria lignaria].
ACCESSION   XP_034177312
VERSION     XP_034177312.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321421.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="vesicle transport protein GOT1B"
                     /calculated_mol_wt=15090
     Region          4..120
                     /region_name="Got1"
                     /note="Got1/Sft2-like family; cl02130"
                     /db_xref="CDD:470475"
     CDS             1..140
                     /gene="LOC117602871"
                     /coded_by="XM_034321421.2:170..592"
                     /db_xref="GeneID:117602871"
ORIGIN      
        1 mfeitdtqki gvglagfgis flflgvlllf dkgllaigni lfisglacvi gprrtlnfff
       61 qkhkikasis flggiivvlm gwpivgmile tygfvllfsg flpvainflr rvpllgtiln
      121 mpglsrildm iagdsnrttv
//
LOCUS       XP_034177323             125 aa            linear   INV 03-SEP-2025
DEFINITION  LITAF domain-containing protein [Osmia lignaria lignaria].
ACCESSION   XP_034177323
VERSION     XP_034177323.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321432.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="LITAF domain-containing protein"
                     /calculated_mol_wt=13201
     Region          54..124
                     /region_name="zf-LITAF-like"
                     /note="LITAF-like zinc ribbon domain; pfam10601"
                     /db_xref="CDD:463165"
     CDS             1..125
                     /gene="LOC117602886"
                     /coded_by="XM_034321432.2:186..563"
                     /db_xref="GeneID:117602886"
ORIGIN      
        1 mhktgppppy epppyappgy sqtmggvppa spftpaetyt ngptivttiv algpgsthti
       61 cphchaeidt ttktepgmia yisgvvialm gcwlgcclip ccidecmdvh hscpnckayl
      121 gryrr
//
LOCUS       XP_034177330             125 aa            linear   INV 03-SEP-2025
DEFINITION  LITAF domain-containing protein [Osmia lignaria lignaria].
ACCESSION   XP_034177330
VERSION     XP_034177330.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321439.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="LITAF domain-containing protein"
                     /calculated_mol_wt=13201
     Region          54..124
                     /region_name="zf-LITAF-like"
                     /note="LITAF-like zinc ribbon domain; pfam10601"
                     /db_xref="CDD:463165"
     CDS             1..125
                     /gene="LOC117602886"
                     /coded_by="XM_034321439.2:166..543"
                     /db_xref="GeneID:117602886"
ORIGIN      
        1 mhktgppppy epppyappgy sqtmggvppa spftpaetyt ngptivttiv algpgsthti
       61 cphchaeidt ttktepgmia yisgvvialm gcwlgcclip ccidecmdvh hscpnckayl
      121 gryrr
//
LOCUS       XP_034177336             125 aa            linear   INV 03-SEP-2025
DEFINITION  LITAF domain-containing protein [Osmia lignaria lignaria].
ACCESSION   XP_034177336
VERSION     XP_034177336.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321445.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="LITAF domain-containing protein"
                     /calculated_mol_wt=13201
     Region          54..124
                     /region_name="zf-LITAF-like"
                     /note="LITAF-like zinc ribbon domain; pfam10601"
                     /db_xref="CDD:463165"
     CDS             1..125
                     /gene="LOC117602886"
                     /coded_by="XM_034321445.2:188..565"
                     /db_xref="GeneID:117602886"
ORIGIN      
        1 mhktgppppy epppyappgy sqtmggvppa spftpaetyt ngptivttiv algpgsthti
       61 cphchaeidt ttktepgmia yisgvvialm gcwlgcclip ccidecmdvh hscpnckayl
      121 gryrr
//
LOCUS       XP_034177344             125 aa            linear   INV 03-SEP-2025
DEFINITION  LITAF domain-containing protein [Osmia lignaria lignaria].
ACCESSION   XP_034177344
VERSION     XP_034177344.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321453.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="LITAF domain-containing protein"
                     /calculated_mol_wt=13201
     Region          54..124
                     /region_name="zf-LITAF-like"
                     /note="LITAF-like zinc ribbon domain; pfam10601"
                     /db_xref="CDD:463165"
     CDS             1..125
                     /gene="LOC117602886"
                     /coded_by="XM_034321453.2:163..540"
                     /db_xref="GeneID:117602886"
ORIGIN      
        1 mhktgppppy epppyappgy sqtmggvppa spftpaetyt ngptivttiv algpgsthti
       61 cphchaeidt ttktepgmia yisgvvialm gcwlgcclip ccidecmdvh hscpnckayl
      121 gryrr
//
LOCUS       XP_034185931            2633 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606952 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185931
VERSION     XP_034185931.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330040.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185931.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2633
                     /product="uncharacterized protein LOC117606952 isoform X1"
                     /calculated_mol_wt=290317
     Region          437..468
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <580..876
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <728..786
                     /region_name="Trypan_PARP"
                     /note="Procyclic acidic repetitive protein (PARP);
                     pfam05887"
                     /db_xref="CDD:368653"
     Region          <731..1148
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          2072..2112
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2077,2091,2102..2103)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2095,2098,2102,2108..2109)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2105..2109
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2117..2150
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2121,2129,2140..2141)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2133,2136,2140,2146..2147)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2143..2147
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2155..2189
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2160,2168,2179..2180)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2172,2175,2179,2185..2186)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2182..2186
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2193..2228
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2198,2207,2218..2219)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2211,2214,2218,2224..2225)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2221..2225
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2230..2264
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2235,2243,2254..2255)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2247,2250,2254,2260..2261)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2257..2261
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2308..2387
                     /region_name="SRCR_2"
                     /note="Scavenger receptor cysteine-rich domain; cl02509"
                     /db_xref="CDD:470597"
     Region          2397..2624
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            order(2435,2481,2576)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(2570,2599,2601)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..2633
                     /gene="LOC117606952"
                     /coded_by="XM_034330040.2:427..8328"
                     /db_xref="GeneID:117606952"
ORIGIN      
        1 maavgmdnpa fvgedectne ttqndnqqqv tldqdrksvs esipvenghq rsfvsqqhve
       61 agrtspethy rtetkievpe snmertetkm ngvhgngnnn dasflnsstt svqtndngkk
      121 eqieavnlel vsmrpyagnn qtkgqeacev padpyeeyfv pvnehrkyis rgpeaevett
      181 vagvrfdlgp gvgreglslr dptaglvdys hwltalrgek lyvtkdkrsr ssywrrmacw
      241 gcgllvllva iiiailaatg vilaqeasep lenleqtksr qfgdvrtags qeyvknppps
      301 pppatssfpp wqttdenryl fvanaldgvl tldqftwdqe lrnhtsemyk afssqienhl
      361 rnamqptmde ttvkvyrimk dgevgfriny ppnsspeetq qrmedallkd anmigqhhvs
      421 rlelskllde cqtswlgcse sckynytkgt facycsdgkm ldadgktcvd endlsnvemd
      481 dapevrtevv hdyaqprsrg pgtvyeprrp dnwdhmdhdl pggghyhtee dshspsysdd
      541 gssepesthv hsedgnhevk deedtvhwmh dhsnhytvep kaepepsdgg epssepepsd
      601 vpersgvaep ssepepssep epssepepaa vepsaepepa avepssepkf aepepsgepr
      661 haepepsaep epsaepkpsa epepsaepep ssvpepsaep kpsaepepsa epepsaepep
      721 ssvpepftep kpsaepepeh stepsaqpep atepsaqpep saepepsaep kpsseaeppi
      781 epkhtteiep saepepagpv epeptsepqa psdepkstag lwpaeepsse pkpseekgss
      841 tepstqpips emepvaepkp snelesstks tvtehpeleq pelsqfphtt ikseeeespv
      901 eqestmpste mihnvehste mqdamqpqtt empssktdss iesmttnkmi vesatelpat
      961 espdlsktpe ssttsseadn tgmpitmeae sttpmvsaet tlpnidksmd emennnapmp
     1021 viplsdeqed skttvvpptd ttmremnnee qmvttiepsk eeiskvdqvt etntpsdett
     1081 eyttistnse entasvtsss sksipettms setasvsmda msenttevns vsdsgtemyn
     1141 etvepkeepk sldlgstsqe anetnvidhi pttifpnlpk nfshniepli eplknntdne
     1201 himiipvvhg tqeqdphsii pelipesiqs snssstrise vttventvqs stektdvvfn
     1261 rstihpeeni vssdpgdsvr ygdnldhsts hplhpavfpe ntvehvteks ddkhaddmsp
     1321 flpdvqkeke vkkaprldkd eqdvpnpfeg hvedvvtdks lvkteertne teatdslndv
     1381 hpevdkehpn ksvengvgrg fkqdaldvle indtesgiqn alnpydvnkp edqrmmksdv
     1441 eedeealkvv pleeeikgte msvkydvdkd ntevtessas vtptddqerr lneaavnntt
     1501 ttsekpeenv vedqyndind dtlskgyqsd dsklhkklad neekllkssn vlfiddktks
     1561 tmnntevttt agsvldkltt eipvlpieiq qdmsttekie sttvaeennk kendsnhqvs
     1621 aeattlqvpt tqmlpmetkt taqvpilpee lqttesdspl ttssirpdmn ttsphmeste
     1681 spihnkteel issevdvqnn qtndmiassm hhfgdnrsin atwlqidvpi lnntneseen
     1741 vttpstthle entknesvaa tsislatttv esykqssdss ssttedvrpv teileddtlp
     1801 vsfdyrtlli tttpktalpd ldvgelseed lrviplektl dlkkkstdkk vvdkykykke
     1861 kelnleehqs envltevpdp atvfstevpr pindiqdhdt sevsksveed sssipklkii
     1921 ettfnrtgtd teqnrtvpas tkkypsfipv seeiiepepv tepfvvirnf ylnrqnadft
     1981 tespamvsea sieghtmmea rniseldsvt ssttesvsss tqdlnemmkd qpvdatvpsl
     2041 iqtkspdtmv estshidvsf lnipenvvfs kcttgqfqcv ngtsrdgayc vplsakcdse
     2101 ndcsdgsdel nckeqgcpen fqcasgqclk rdlvcnrivd cddgsdeinc eewkcqfdef
     2161 kcpsgrcipg iwqcngrpdc edhrdeynca escgneeylc ptekwciplt whcngineca
     2221 ngedeklcdc gidqfkcqtg gcipenlvcd giehcpdhsd ewncltmntg pernltdvqk
     2281 eteesngaqe ltestsllki rqfngesrlv csdgwseefs dsycqslgfs gseatdlqtw
     2341 dknqkilrlk anpnhrmplv anleqvefcv sdkvvqvscq efscgshyge gptarlvggt
     2401 pasegqwssv allkepkqga actasilgpm hviasyscth rlkqtngwql ftdknllkpl
     2461 pvksiipypq vkynqflynn dmvlvelekp ltfsrnvsav clpkdpiqpr qicvtvgwgf
     2521 pvngevdlqk yltflpvpty dsekcnatsh yagfitkdni cagftdtdkg pcyndegapl
     2581 mcvsesggsa rweiqgllsh hsrcsrghpa iyssvgpals wlrnsvpalq tqs
//
LOCUS       XP_034185937            2606 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606952 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185937
VERSION     XP_034185937.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330046.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185937.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2606
                     /product="uncharacterized protein LOC117606952 isoform X2"
                     /calculated_mol_wt=287291
     Region          410..441
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <553..849
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <701..759
                     /region_name="Trypan_PARP"
                     /note="Procyclic acidic repetitive protein (PARP);
                     pfam05887"
                     /db_xref="CDD:368653"
     Region          <704..1121
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          2045..2085
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2050,2064,2075..2076)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2068,2071,2075,2081..2082)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2078..2082
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2090..2123
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2094,2102,2113..2114)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2106,2109,2113,2119..2120)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2116..2120
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2128..2162
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2133,2141,2152..2153)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2145,2148,2152,2158..2159)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2155..2159
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2166..2201
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2171,2180,2191..2192)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2184,2187,2191,2197..2198)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2194..2198
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2203..2237
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2208,2216,2227..2228)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2220,2223,2227,2233..2234)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2230..2234
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2281..2360
                     /region_name="SRCR_2"
                     /note="Scavenger receptor cysteine-rich domain; cl02509"
                     /db_xref="CDD:470597"
     Region          2370..2597
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            order(2408,2454,2549)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(2543,2572,2574)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..2606
                     /gene="LOC117606952"
                     /coded_by="XM_034330046.2:428..8248"
                     /db_xref="GeneID:117606952"
ORIGIN      
        1 maavgmdnpa fvgedectne ttqndnqqqq hveagrtspe thyrtetkie vpesnmerte
       61 tkmngvhgng nnndasflns sttsvqtndn gkkeqieavn lelvsmrpya gnnqtkgqea
      121 cevpadpyee yfvpvnehrk yisrgpeaev ettvagvrfd lgpgvgregl slrdptaglv
      181 dyshwltalr geklyvtkdk rsrssywrrm acwgcgllvl lvaiiiaila atgvilaqea
      241 seplenleqt ksrqfgdvrt agsqeyvknp ppspppatss fppwqttden rylfvanald
      301 gvltldqftw dqelrnhtse mykafssqie nhlrnamqpt mdettvkvyr imkdgevgfr
      361 inyppnsspe etqqrmedal lkdanmigqh hvsrlelskl ldecqtswlg csesckynyt
      421 kgtfacycsd gkmldadgkt cvdendlsnv emddapevrt evvhdyaqpr srgpgtvyep
      481 rrpdnwdhmd hdlpggghyh teedshspsy sddgssepes thvhsedgnh evkdeedtvh
      541 wmhdhsnhyt vepkaepeps dggepssepe psdvpersgv aepssepeps sepepssepe
      601 paavepsaep epaavepsse pkfaepepsg eprhaepeps aepepsaepk psaepepsae
      661 pepssvpeps aepkpsaepe psaepepsae pepssvpepf tepkpsaepe pehstepsaq
      721 pepatepsaq pepsaepeps aepkpsseae ppiepkhtte iepsaepepa gpvepeptse
      781 pqapsdepks taglwpaeep ssepkpseek gsstepstqp ipsemepvae pkpsneless
      841 tkstvtehpe leqpelsqfp httikseeee spveqestmp stemihnveh stemqdamqp
      901 qttempsskt dssiesmttn kmivesatel patespdlsk tpessttsse adntgmpitm
      961 eaesttpmvs aettlpnidk smdemennna pmpviplsde qedskttvvp ptdttmremn
     1021 neeqmvttie pskeeiskvd qvtetntpsd etteyttist nseentasvt ssssksipet
     1081 tmssetasvs mdamsentte vnsvsdsgte mynetvepke epksldlgst sqeanetnvi
     1141 dhipttifpn lpknfshnie plieplknnt dnehimiipv vhgtqeqdph siipelipes
     1201 iqssnssstr isevttvent vqsstektdv vfnrstihpe enivssdpgd svrygdnldh
     1261 stshplhpav fpentvehvt eksddkhadd mspflpdvqk ekevkkaprl dkdeqdvpnp
     1321 feghvedvvt dkslvkteer tneteatdsl ndvhpevdke hpnksvengv grgfkqdald
     1381 vleindtesg iqnalnpydv nkpedqrmmk sdveedeeal kvvpleeeik gtemsvkydv
     1441 dkdntevtes sasvtptddq errlneaavn nttttsekpe envvedqynd inddtlskgy
     1501 qsddsklhkk ladneekllk ssnvlfiddk tkstmnntev tttagsvldk ltteipvlpi
     1561 eiqqdmstte kiesttvaee nnkkendsnh qvsaeattlq vpttqmlpme tkttaqvpil
     1621 peelqttesd splttssirp dmnttsphme stespihnkt eelissevdv qnnqtndmia
     1681 ssmhhfgdnr sinatwlqid vpilnntnes eenvttpstt hleentknes vaatsislat
     1741 ttvesykqss dssssttedv rpvteiledd tlpvsfdyrt llitttpkta lpdldvgels
     1801 eedlrviple ktldlkkkst dkkvvdkyky kkekelnlee hqsenvltev pdpatvfste
     1861 vprpindiqd hdtsevsksv eedsssipkl kiiettfnrt gtdteqnrtv pastkkypsf
     1921 ipvseeiiep epvtepfvvi rnfylnrqna dfttespamv seasieghtm mearniseld
     1981 svtssttesv ssstqdlnem mkdqpvdatv psliqtkspd tmvestshid vsflnipenv
     2041 vfskcttgqf qcvngtsrdg aycvplsakc dsendcsdgs delnckeqgc penfqcasgq
     2101 clkrdlvcnr ivdcddgsde inceewkcqf defkcpsgrc ipgiwqcngr pdcedhrdey
     2161 ncaescgnee ylcptekwci pltwhcngin ecangedekl cdcgidqfkc qtggcipenl
     2221 vcdgiehcpd hsdewncltm ntgpernltd vqketeesng aqeltestsl lkirqfnges
     2281 rlvcsdgwse efsdsycqsl gfsgseatdl qtwdknqkil rlkanpnhrm plvanleqve
     2341 fcvsdkvvqv scqefscgsh ygegptarlv ggtpasegqw ssvallkepk qgaactasil
     2401 gpmhviasys cthrlkqtng wqlftdknll kplpvksiip ypqvkynqfl ynndmvlvel
     2461 ekpltfsrnv savclpkdpi qprqicvtvg wgfpvngevd lqkyltflpv ptydsekcna
     2521 tshyagfitk dnicagftdt dkgpcyndeg aplmcvsesg gsarweiqgl lshhsrcsrg
     2581 hpaiyssvgp alswlrnsvp alqtqs
//
LOCUS       XP_034185947            2586 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606952 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185947
VERSION     XP_034185947.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330056.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185947.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2586
                     /product="uncharacterized protein LOC117606952 isoform X3"
                     /calculated_mol_wt=285381
     Region          390..421
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          <533..829
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <681..739
                     /region_name="Trypan_PARP"
                     /note="Procyclic acidic repetitive protein (PARP);
                     pfam05887"
                     /db_xref="CDD:368653"
     Region          <684..1101
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          2025..2065
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2030,2044,2055..2056)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2048,2051,2055,2061..2062)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2058..2062
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2070..2103
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2074,2082,2093..2094)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2086,2089,2093,2099..2100)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2096..2100
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2108..2142
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2113,2121,2132..2133)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2125,2128,2132,2138..2139)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2135..2139
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2146..2181
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2151,2160,2171..2172)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2164,2167,2171,2177..2178)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2174..2178
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2183..2217
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(2188,2196,2207..2208)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(2200,2203,2207,2213..2214)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            2210..2214
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          2261..2340
                     /region_name="SRCR_2"
                     /note="Scavenger receptor cysteine-rich domain; cl02509"
                     /db_xref="CDD:470597"
     Region          2350..2577
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            order(2388,2434,2529)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(2523,2552,2554)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..2586
                     /gene="LOC117606952"
                     /coded_by="XM_034330056.2:424..8184"
                     /db_xref="GeneID:117606952"
ORIGIN      
        1 maavgmdnpa fvgedectne ttqndnqqqv tldqdrksvs esipvenghq rsfvsqqhve
       61 agrtspethy rtetkievpe snmertetkm ngvhgngnnn dasflnsstt svqtndngkk
      121 eqieavnlel vsmrpyagnn qtkgqeacev padpyeeyfv pvnehrkyir geklyvtkdk
      181 rsrssywrrm acwgcgllvl lvaiiiaila atgvilaqea seplenleqt ksrqfgdvrt
      241 agsqeyvknp ppspppatss fppwqttden rylfvanald gvltldqftw dqelrnhtse
      301 mykafssqie nhlrnamqpt mdettvkvyr imkdgevgfr inyppnsspe etqqrmedal
      361 lkdanmigqh hvsrlelskl ldecqtswlg csesckynyt kgtfacycsd gkmldadgkt
      421 cvdendlsnv emddapevrt evvhdyaqpr srgpgtvyep rrpdnwdhmd hdlpggghyh
      481 teedshspsy sddgssepes thvhsedgnh evkdeedtvh wmhdhsnhyt vepkaepeps
      541 dggepssepe psdvpersgv aepssepeps sepepssepe paavepsaep epaavepsse
      601 pkfaepepsg eprhaepeps aepepsaepk psaepepsae pepssvpeps aepkpsaepe
      661 psaepepsae pepssvpepf tepkpsaepe pehstepsaq pepatepsaq pepsaepeps
      721 aepkpsseae ppiepkhtte iepsaepepa gpvepeptse pqapsdepks taglwpaeep
      781 ssepkpseek gsstepstqp ipsemepvae pkpsneless tkstvtehpe leqpelsqfp
      841 httikseeee spveqestmp stemihnveh stemqdamqp qttempsskt dssiesmttn
      901 kmivesatel patespdlsk tpessttsse adntgmpitm eaesttpmvs aettlpnidk
      961 smdemennna pmpviplsde qedskttvvp ptdttmremn neeqmvttie pskeeiskvd
     1021 qvtetntpsd etteyttist nseentasvt ssssksipet tmssetasvs mdamsentte
     1081 vnsvsdsgte mynetvepke epksldlgst sqeanetnvi dhipttifpn lpknfshnie
     1141 plieplknnt dnehimiipv vhgtqeqdph siipelipes iqssnssstr isevttvent
     1201 vqsstektdv vfnrstihpe enivssdpgd svrygdnldh stshplhpav fpentvehvt
     1261 eksddkhadd mspflpdvqk ekevkkaprl dkdeqdvpnp feghvedvvt dkslvkteer
     1321 tneteatdsl ndvhpevdke hpnksvengv grgfkqdald vleindtesg iqnalnpydv
     1381 nkpedqrmmk sdveedeeal kvvpleeeik gtemsvkydv dkdntevtes sasvtptddq
     1441 errlneaavn nttttsekpe envvedqynd inddtlskgy qsddsklhkk ladneekllk
     1501 ssnvlfiddk tkstmnntev tttagsvldk ltteipvlpi eiqqdmstte kiesttvaee
     1561 nnkkendsnh qvsaeattlq vpttqmlpme tkttaqvpil peelqttesd splttssirp
     1621 dmnttsphme stespihnkt eelissevdv qnnqtndmia ssmhhfgdnr sinatwlqid
     1681 vpilnntnes eenvttpstt hleentknes vaatsislat ttvesykqss dssssttedv
     1741 rpvteiledd tlpvsfdyrt llitttpkta lpdldvgels eedlrviple ktldlkkkst
     1801 dkkvvdkyky kkekelnlee hqsenvltev pdpatvfste vprpindiqd hdtsevsksv
     1861 eedsssipkl kiiettfnrt gtdteqnrtv pastkkypsf ipvseeiiep epvtepfvvi
     1921 rnfylnrqna dfttespamv seasieghtm mearniseld svtssttesv ssstqdlnem
     1981 mkdqpvdatv psliqtkspd tmvestshid vsflnipenv vfskcttgqf qcvngtsrdg
     2041 aycvplsakc dsendcsdgs delnckeqgc penfqcasgq clkrdlvcnr ivdcddgsde
     2101 inceewkcqf defkcpsgrc ipgiwqcngr pdcedhrdey ncaescgnee ylcptekwci
     2161 pltwhcngin ecangedekl cdcgidqfkc qtggcipenl vcdgiehcpd hsdewncltm
     2221 ntgpernltd vqketeesng aqeltestsl lkirqfnges rlvcsdgwse efsdsycqsl
     2281 gfsgseatdl qtwdknqkil rlkanpnhrm plvanleqve fcvsdkvvqv scqefscgsh
     2341 ygegptarlv ggtpasegqw ssvallkepk qgaactasil gpmhviasys cthrlkqtng
     2401 wqlftdknll kplpvksiip ypqvkynqfl ynndmvlvel ekpltfsrnv savclpkdpi
     2461 qprqicvtvg wgfpvngevd lqkyltflpv ptydsekcna tshyagfitk dnicagftdt
     2521 dkgpcyndeg aplmcvsesg gsarweiqgl lshhsrcsrg hpaiyssvgp alswlrnsvp
     2581 alqtqs
//
LOCUS       XP_034185960             728 aa            linear   INV 03-SEP-2025
DEFINITION  organic anion transporting polypeptide 74D [Osmia lignaria
            lignaria].
ACCESSION   XP_034185960
VERSION     XP_034185960.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330069.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185960.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="organic anion transporting polypeptide 74D"
                     /calculated_mol_wt=79536
     Region          98..667
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..728
                     /gene="Oatp74D"
                     /coded_by="XM_034330069.2:2048..4234"
                     /db_xref="GeneID:117606966"
ORIGIN      
        1 magedtqila ngnvealtii ppkmangngl icgkqhnnng sipngklhei sgvengklim
       61 sdekssslht efddadvscg wgpcrphwlq yfatkqaflv tfcitwvlqg myytyfvsvi
      121 ttieklfqiq skttgiimsa teigqigssl lltyyggqgh rpkwiawgmi lfavssftcs
      181 mphfifgeql ihqnssdpmp ahfcslqdrf enstfdslns stttshvntm hncegdslte
      241 qriqskittv vlaiffvsll gvgmgqtavy tlgipyiddn vanresplyf aitigvrilg
      301 palgfilgsl ctmiyadlsv npqitptdpr wvgawwlglv lisamlmlvs igmfafptrl
      361 pasrtppkra dakkpslrdf pkavkrllkn dilmfrtass vlhilpiagl ytflpkyles
      421 qfrlpahhan misgvggilv mglgiiisgv filrakpnar fvaawiafta vvyaigmgvl
      481 mfigcpmddf aglvshsdgl ssfeptcdas cdcvrnkfsp icgadgktyf sachagcfny
      541 tvvdgkvasf yncqcigtnv ttpeilstat igycelecsn fwvymvlfsv fvfihstsev
      601 gsmllilrcv dprdkamalg liqfaiglfg nvpcpivyga vvdsaclvwe yacgergacw
      661 lydsnvfrmf yhgttggilv lafvvdivvw ykagsisfve eqeaeegtve emttlksqda
      721 qavdndyl
//
LOCUS       XP_034185966             728 aa            linear   INV 03-SEP-2025
DEFINITION  organic anion transporting polypeptide 74D [Osmia lignaria
            lignaria].
ACCESSION   XP_034185966
VERSION     XP_034185966.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330075.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185966.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="organic anion transporting polypeptide 74D"
                     /calculated_mol_wt=79536
     Region          98..667
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..728
                     /gene="Oatp74D"
                     /coded_by="XM_034330075.2:317..2503"
                     /db_xref="GeneID:117606966"
ORIGIN      
        1 magedtqila ngnvealtii ppkmangngl icgkqhnnng sipngklhei sgvengklim
       61 sdekssslht efddadvscg wgpcrphwlq yfatkqaflv tfcitwvlqg myytyfvsvi
      121 ttieklfqiq skttgiimsa teigqigssl lltyyggqgh rpkwiawgmi lfavssftcs
      181 mphfifgeql ihqnssdpmp ahfcslqdrf enstfdslns stttshvntm hncegdslte
      241 qriqskittv vlaiffvsll gvgmgqtavy tlgipyiddn vanresplyf aitigvrilg
      301 palgfilgsl ctmiyadlsv npqitptdpr wvgawwlglv lisamlmlvs igmfafptrl
      361 pasrtppkra dakkpslrdf pkavkrllkn dilmfrtass vlhilpiagl ytflpkyles
      421 qfrlpahhan misgvggilv mglgiiisgv filrakpnar fvaawiafta vvyaigmgvl
      481 mfigcpmddf aglvshsdgl ssfeptcdas cdcvrnkfsp icgadgktyf sachagcfny
      541 tvvdgkvasf yncqcigtnv ttpeilstat igycelecsn fwvymvlfsv fvfihstsev
      601 gsmllilrcv dprdkamalg liqfaiglfg nvpcpivyga vvdsaclvwe yacgergacw
      661 lydsnvfrmf yhgttggilv lafvvdivvw ykagsisfve eqeaeegtve emttlksqda
      721 qavdndyl
//
LOCUS       XP_076546252             728 aa            linear   INV 03-SEP-2025
DEFINITION  organic anion transporting polypeptide 74D [Osmia lignaria
            lignaria].
ACCESSION   XP_076546252
VERSION     XP_076546252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690137.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="organic anion transporting polypeptide 74D"
                     /calculated_mol_wt=79536
     Region          98..667
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..728
                     /gene="Oatp74D"
                     /coded_by="XM_076690137.1:361..2547"
                     /db_xref="GeneID:117606966"
ORIGIN      
        1 magedtqila ngnvealtii ppkmangngl icgkqhnnng sipngklhei sgvengklim
       61 sdekssslht efddadvscg wgpcrphwlq yfatkqaflv tfcitwvlqg myytyfvsvi
      121 ttieklfqiq skttgiimsa teigqigssl lltyyggqgh rpkwiawgmi lfavssftcs
      181 mphfifgeql ihqnssdpmp ahfcslqdrf enstfdslns stttshvntm hncegdslte
      241 qriqskittv vlaiffvsll gvgmgqtavy tlgipyiddn vanresplyf aitigvrilg
      301 palgfilgsl ctmiyadlsv npqitptdpr wvgawwlglv lisamlmlvs igmfafptrl
      361 pasrtppkra dakkpslrdf pkavkrllkn dilmfrtass vlhilpiagl ytflpkyles
      421 qfrlpahhan misgvggilv mglgiiisgv filrakpnar fvaawiafta vvyaigmgvl
      481 mfigcpmddf aglvshsdgl ssfeptcdas cdcvrnkfsp icgadgktyf sachagcfny
      541 tvvdgkvasf yncqcigtnv ttpeilstat igycelecsn fwvymvlfsv fvfihstsev
      601 gsmllilrcv dprdkamalg liqfaiglfg nvpcpivyga vvdsaclvwe yacgergacw
      661 lydsnvfrmf yhgttggilv lafvvdivvw ykagsisfve eqeaeegtve emttlksqda
      721 qavdndyl
//
LOCUS       XP_076546253             728 aa            linear   INV 03-SEP-2025
DEFINITION  organic anion transporting polypeptide 74D [Osmia lignaria
            lignaria].
ACCESSION   XP_076546253
VERSION     XP_076546253.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690138.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="organic anion transporting polypeptide 74D"
                     /calculated_mol_wt=79536
     Region          98..667
                     /region_name="OATP"
                     /note="Organic Anion Transporter Polypeptide (OATP)
                     family; pfam03137"
                     /db_xref="CDD:460821"
     CDS             1..728
                     /gene="Oatp74D"
                     /coded_by="XM_076690138.1:2092..4278"
                     /db_xref="GeneID:117606966"
ORIGIN      
        1 magedtqila ngnvealtii ppkmangngl icgkqhnnng sipngklhei sgvengklim
       61 sdekssslht efddadvscg wgpcrphwlq yfatkqaflv tfcitwvlqg myytyfvsvi
      121 ttieklfqiq skttgiimsa teigqigssl lltyyggqgh rpkwiawgmi lfavssftcs
      181 mphfifgeql ihqnssdpmp ahfcslqdrf enstfdslns stttshvntm hncegdslte
      241 qriqskittv vlaiffvsll gvgmgqtavy tlgipyiddn vanresplyf aitigvrilg
      301 palgfilgsl ctmiyadlsv npqitptdpr wvgawwlglv lisamlmlvs igmfafptrl
      361 pasrtppkra dakkpslrdf pkavkrllkn dilmfrtass vlhilpiagl ytflpkyles
      421 qfrlpahhan misgvggilv mglgiiisgv filrakpnar fvaawiafta vvyaigmgvl
      481 mfigcpmddf aglvshsdgl ssfeptcdas cdcvrnkfsp icgadgktyf sachagcfny
      541 tvvdgkvasf yncqcigtnv ttpeilstat igycelecsn fwvymvlfsv fvfihstsev
      601 gsmllilrcv dprdkamalg liqfaiglfg nvpcpivyga vvdsaclvwe yacgergacw
      661 lydsnvfrmf yhgttggilv lafvvdivvw ykagsisfve eqeaeegtve emttlksqda
      721 qavdndyl
//
LOCUS       XP_034185997             578 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606988 [Osmia lignaria lignaria].
ACCESSION   XP_034185997
VERSION     XP_034185997.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330106.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185997.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="uncharacterized protein LOC117606988"
                     /calculated_mol_wt=65545
     Region          <46..>87
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     Region          <438..>526
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     CDS             1..578
                     /gene="LOC117606988"
                     /coded_by="XM_034330106.2:858..2594"
                     /db_xref="GeneID:117606988"
ORIGIN      
        1 makilrtlyl valfvslvkc qgsllqmsqe lwtklltggf sktgkldplk vpvikvdqse
       61 gdtsyriilr nveitglnes tldsihiarg rlksnlsele agyvsysdlr dldsiryrfh
      121 tlikepklqn esfeaivsat nqeskimtsp edsrfdrfrq ydpfllkiqa dkmkekpers
      181 enspqdsnyg tfsmegakvv skqdtsqiva nmrypadvqm iyamsainaa lnarnqeqre
      241 mydqgrsasf gerrgeares qrrgyhsgrr setryedpet sasenverrq dprvfppyig
      301 reqvyqgenr rlekqpgyid ivyadgnnng svkrfgngri eskqdaqvfg mkdvrkeile
      361 grkgishnct egepltkrne avkaafdskd lnnltryarn yqekegyfee gmqllyhygg
      421 npanekvelg krykrahken etdddvmhvi lkirapelri ksdyqlngkv gkelirgtgk
      481 lsgnfselvg dftielkkmk dqdvlivraa rsklnakkqn vslqgmdeeg pvksilthgl
      541 maaeavaaml addlatkaln drtadamiyk myknlpvn
//
LOCUS       XP_034186007             578 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606988 [Osmia lignaria lignaria].
ACCESSION   XP_034186007
VERSION     XP_034186007.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330116.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186007.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="uncharacterized protein LOC117606988"
                     /calculated_mol_wt=65545
     Region          <46..>87
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     Region          <438..>526
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     CDS             1..578
                     /gene="LOC117606988"
                     /coded_by="XM_034330116.2:846..2582"
                     /db_xref="GeneID:117606988"
ORIGIN      
        1 makilrtlyl valfvslvkc qgsllqmsqe lwtklltggf sktgkldplk vpvikvdqse
       61 gdtsyriilr nveitglnes tldsihiarg rlksnlsele agyvsysdlr dldsiryrfh
      121 tlikepklqn esfeaivsat nqeskimtsp edsrfdrfrq ydpfllkiqa dkmkekpers
      181 enspqdsnyg tfsmegakvv skqdtsqiva nmrypadvqm iyamsainaa lnarnqeqre
      241 mydqgrsasf gerrgeares qrrgyhsgrr setryedpet sasenverrq dprvfppyig
      301 reqvyqgenr rlekqpgyid ivyadgnnng svkrfgngri eskqdaqvfg mkdvrkeile
      361 grkgishnct egepltkrne avkaafdskd lnnltryarn yqekegyfee gmqllyhygg
      421 npanekvelg krykrahken etdddvmhvi lkirapelri ksdyqlngkv gkelirgtgk
      481 lsgnfselvg dftielkkmk dqdvlivraa rsklnakkqn vslqgmdeeg pvksilthgl
      541 maaeavaaml addlatkaln drtadamiyk myknlpvn
//
LOCUS       XP_076546254             578 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606988 [Osmia lignaria lignaria].
ACCESSION   XP_076546254
VERSION     XP_076546254.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690139.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="uncharacterized protein LOC117606988"
                     /calculated_mol_wt=65545
     Region          <46..>87
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     Region          <438..>526
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     cl12117"
                     /db_xref="CDD:472279"
     CDS             1..578
                     /gene="LOC117606988"
                     /coded_by="XM_076690139.1:1084..2820"
                     /db_xref="GeneID:117606988"
ORIGIN      
        1 makilrtlyl valfvslvkc qgsllqmsqe lwtklltggf sktgkldplk vpvikvdqse
       61 gdtsyriilr nveitglnes tldsihiarg rlksnlsele agyvsysdlr dldsiryrfh
      121 tlikepklqn esfeaivsat nqeskimtsp edsrfdrfrq ydpfllkiqa dkmkekpers
      181 enspqdsnyg tfsmegakvv skqdtsqiva nmrypadvqm iyamsainaa lnarnqeqre
      241 mydqgrsasf gerrgeares qrrgyhsgrr setryedpet sasenverrq dprvfppyig
      301 reqvyqgenr rlekqpgyid ivyadgnnng svkrfgngri eskqdaqvfg mkdvrkeile
      361 grkgishnct egepltkrne avkaafdskd lnnltryarn yqekegyfee gmqllyhygg
      421 npanekvelg krykrahken etdddvmhvi lkirapelri ksdyqlngkv gkelirgtgk
      481 lsgnfselvg dftielkkmk dqdvlivraa rsklnakkqn vslqgmdeeg pvksilthgl
      541 maaeavaaml addlatkaln drtadamiyk myknlpvn
//
LOCUS       XP_034186021             497 aa            linear   INV 03-SEP-2025
DEFINITION  MFS-type transporter SLC18B1 [Osmia lignaria lignaria].
ACCESSION   XP_034186021
VERSION     XP_034186021.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330130.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186021.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="MFS-type transporter SLC18B1"
                     /calculated_mol_wt=53120
     Region          13..404
                     /region_name="MFS_SLC18B1"
                     /note="Solute carrier family 18 member B1 of the Major
                     Facilitator Superfamily of transporters; cd17385"
                     /db_xref="CDD:340943"
     Site            order(23..24,27..28,31,63,120..121,123..125,128,147,
                     150..151,154,224,227..228,231..233,236,263,267,323..324,
                     328,332,355,358..359,362..363,366)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340943"
     CDS             1..497
                     /gene="LOC117606996"
                     /coded_by="XM_034330130.2:280..1773"
                     /db_xref="GeneID:117606996"
ORIGIN      
        1 mmaqftkrqw ltlivislad fanaicvslq apfypqeaek kgaspseygl vfgvfelivf
       61 iisplygryl hrigpkllfn ggilttgtca iffglldkvn ghypfivlsf viriieamgn
      121 aafltasfai iakefpdnva ttfasletff glglivgptv ggalyqvggy ttpfvvlgsa
      181 lfitavmtif ilpvhpnnne aaqdtggvtr alkipgvlia tasiiatsms igflqatlep
      241 hlrqfnlspv ilglmfving gtyaitapaw gwfcdkhfhp kvatvagcml vvvgfclvgp
      301 apfipcqttl wmtvcglvvh glgmaaqlva sftdalrtsi sygfpnnlet yglisglwts
      361 tfalgafigp cvagilldnt gfrngtmfiv mlhllvgiia tvflssctgk rkpytevgas
      421 ddlrapltds sqsrngsmry gsrgqgvpid rpsgmnslis cnsysnraga wsrasysgry
      481 shsygsieak ryleitt
//
LOCUS       XP_076546255             450 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607214 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546255
VERSION     XP_076546255.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690140.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="uncharacterized protein LOC117607214 isoform X1"
                     /calculated_mol_wt=49281
     Region          22..256
                     /region_name="DUF4820"
                     /note="Domain of unknown function (DUF4820); pfam16091"
                     /db_xref="CDD:465015"
     CDS             1..450
                     /gene="LOC117607214"
                     /coded_by="XM_076690140.1:149..1501"
                     /db_xref="GeneID:117607214"
ORIGIN      
        1 menegspsvi inestegirg wiesvvitas qtsvgqalfk lldsflwvve ksaqwslpsh
       61 evaaeengkv fgkielvrpl pwvlflpglv ilriirvgln vgafvlgypk iqpsgmvkfv
      121 qkgrrrlraf nlkavksvrr tskdkrltmi eakkalirsi rltlstlscl dssnsspspp
      181 ptkihvskme lepmatpdek sttesvgspi ntkikrkfse vssdnestde setetldtkl
      241 erlalesstd dpdfnpaecs tesssassen evekemsfde lvevqeeake fynsgeekpl
      301 iislenlkrn srkdeelgee vkhvdssesk vetvssitne fingeicsti dtrltvlpee
      361 vpppapvlap apvpapipsp asepvpvpvp vpvpvpapap eviekvspkk sppqipaash
      421 srnfiakens stiperkgyk hkkpgrretk
//
LOCUS       XP_076546256             450 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607214 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546256
VERSION     XP_076546256.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690141.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="uncharacterized protein LOC117607214 isoform X1"
                     /calculated_mol_wt=49281
     Region          22..256
                     /region_name="DUF4820"
                     /note="Domain of unknown function (DUF4820); pfam16091"
                     /db_xref="CDD:465015"
     CDS             1..450
                     /gene="LOC117607214"
                     /coded_by="XM_076690141.1:154..1506"
                     /db_xref="GeneID:117607214"
ORIGIN      
        1 menegspsvi inestegirg wiesvvitas qtsvgqalfk lldsflwvve ksaqwslpsh
       61 evaaeengkv fgkielvrpl pwvlflpglv ilriirvgln vgafvlgypk iqpsgmvkfv
      121 qkgrrrlraf nlkavksvrr tskdkrltmi eakkalirsi rltlstlscl dssnsspspp
      181 ptkihvskme lepmatpdek sttesvgspi ntkikrkfse vssdnestde setetldtkl
      241 erlalesstd dpdfnpaecs tesssassen evekemsfde lvevqeeake fynsgeekpl
      301 iislenlkrn srkdeelgee vkhvdssesk vetvssitne fingeicsti dtrltvlpee
      361 vpppapvlap apvpapipsp asepvpvpvp vpvpvpapap eviekvspkk sppqipaash
      421 srnfiakens stiperkgyk hkkpgrretk
//
LOCUS       XP_076546257             447 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607214 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546257
VERSION     XP_076546257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690142.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..447
                     /product="uncharacterized protein LOC117607214 isoform X2"
                     /calculated_mol_wt=48972
     Region          22..256
                     /region_name="DUF4820"
                     /note="Domain of unknown function (DUF4820); pfam16091"
                     /db_xref="CDD:465015"
     CDS             1..447
                     /gene="LOC117607214"
                     /coded_by="XM_076690142.1:149..1492"
                     /db_xref="GeneID:117607214"
ORIGIN      
        1 menegspsvi inestegirg wiesvvitas qtsvgqalfk lldsflwvve ksaqwslpsh
       61 evaaeengkv fgkielvrpl pwvlflpglv ilriirvgln vgafvlgypk iqpsgmvkfv
      121 qkgrrrlraf nlkavksvrr tskdkrltmi eakkalirsi rltlstlscl dssnsspspp
      181 ptkihvskme lepmatpdek sttesvgspi ntkikrkfse vssdnestde setetldtkl
      241 erlalesstd dpdfnpaecs tesssassen evekemsfde lvevqeeake fynsgeekpl
      301 iislenlkrn srkdeelgee vkhvdssesk vetvssitne fingeicsti dtrlteevpp
      361 papvlapapv papipspase pvpvpvpvpv pvpapapevi ekvspkkspp qipaashsrn
      421 fiakenssti perkgykhkk pgrretk
//
LOCUS       XP_076546258             446 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607214 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546258
VERSION     XP_076546258.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690143.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="uncharacterized protein LOC117607214 isoform X3"
                     /calculated_mol_wt=48843
     Region          22..256
                     /region_name="DUF4820"
                     /note="Domain of unknown function (DUF4820); pfam16091"
                     /db_xref="CDD:465015"
     CDS             1..446
                     /gene="LOC117607214"
                     /coded_by="XM_076690143.1:149..1489"
                     /db_xref="GeneID:117607214"
ORIGIN      
        1 menegspsvi inestegirg wiesvvitas qtsvgqalfk lldsflwvve ksaqwslpsh
       61 evaaeengkv fgkielvrpl pwvlflpglv ilriirvgln vgafvlgypk iqpsgmvkfv
      121 qkgrrrlraf nlkavksvrr tskdkrltmi eakkalirsi rltlstlscl dssnsspspp
      181 ptkihvskme lepmatpdek sttesvgspi ntkikrkfse vssdnestde setetldtkl
      241 erlalesstd dpdfnpaecs tesssassen evekemsfde lvevqeeake fynsgeekpl
      301 iislenlkrn srkdeelgee vkhvdssesk vetvssitne fingeicsti dtrltevppp
      361 apvlapapvp apipspasep vpvpvpvpvp vpapapevie kvspkksppq ipaashsrnf
      421 iakensstip erkgykhkkp grretk
//
LOCUS       XP_034186050             450 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor E2F3 [Osmia lignaria lignaria].
ACCESSION   XP_034186050
VERSION     XP_034186050.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330159.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="transcription factor E2F3"
                     /calculated_mol_wt=50046
     Region          135..199
                     /region_name="E2F_TDP"
                     /note="E2F/DP family winged-helix DNA-binding domain;
                     pfam02319"
                     /db_xref="CDD:460530"
     Region          207..309
                     /region_name="E2F_DD"
                     /note="Dimerization domain of E2F transcription factors;
                     cd14660"
                     /db_xref="CDD:271137"
     Region          207..242
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:271137"
     Site            order(208..209,212..213,215..216,219..220,222..223,
                     226..227,229..230,232..234,236..237,240,245..250,253..254,
                     257,261..269,289..296,298..301,303,309)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:271137"
     Site            order(271..280,284..285,290)
                     /site_type="other"
                     /note="RbC binding site [polypeptide binding]"
                     /db_xref="CDD:271137"
     CDS             1..450
                     /gene="LOC117607003"
                     /coded_by="XM_034330159.2:329..1681"
                     /db_xref="GeneID:117607003"
ORIGIN      
        1 mprvrrqvvl edparpvtpd mvetklmkte flddgsldev qaqkvaeetp sphlqdhqyg
       61 qtpcyqvtrk ptqppprteq isvqavkrrl nlemgatgps qsafkaprgk rrrsgsnslt
      121 ghtptksktv ertrydtsls lltkkfihlv essqdgvvdl nvaseklevq krriyditnv
      181 legigilekk sknniqwkgg qlpndrndia dlrrevadle akentldrli hgadknlrel
      241 cadrqyayvt yhdlrsvpmy kdqaimavka ppeatlhvpq pinnlgqqkl qmhmrsshge
      301 ievflcpddp tvktspnpgy tttqpvpssk eseipdlppe llvnggsgvr vepvpsvest
      361 lntrsctpvv stvtslagmr dallcesddy gpmgggkfql qtedqintpd lsffefgehl
      421 lslepplsen dysfslgtee glsdlfdfkf
//
LOCUS       XP_034186034             450 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor E2F3 [Osmia lignaria lignaria].
ACCESSION   XP_034186034
VERSION     XP_034186034.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="transcription factor E2F3"
                     /calculated_mol_wt=50046
     Region          135..199
                     /region_name="E2F_TDP"
                     /note="E2F/DP family winged-helix DNA-binding domain;
                     pfam02319"
                     /db_xref="CDD:460530"
     Region          207..309
                     /region_name="E2F_DD"
                     /note="Dimerization domain of E2F transcription factors;
                     cd14660"
                     /db_xref="CDD:271137"
     Region          207..242
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:271137"
     Site            order(208..209,212..213,215..216,219..220,222..223,
                     226..227,229..230,232..234,236..237,240,245..250,253..254,
                     257,261..269,289..296,298..301,303,309)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:271137"
     Site            order(271..280,284..285,290)
                     /site_type="other"
                     /note="RbC binding site [polypeptide binding]"
                     /db_xref="CDD:271137"
     CDS             1..450
                     /gene="LOC117607003"
                     /coded_by="XM_034330143.2:405..1757"
                     /db_xref="GeneID:117607003"
ORIGIN      
        1 mprvrrqvvl edparpvtpd mvetklmkte flddgsldev qaqkvaeetp sphlqdhqyg
       61 qtpcyqvtrk ptqppprteq isvqavkrrl nlemgatgps qsafkaprgk rrrsgsnslt
      121 ghtptksktv ertrydtsls lltkkfihlv essqdgvvdl nvaseklevq krriyditnv
      181 legigilekk sknniqwkgg qlpndrndia dlrrevadle akentldrli hgadknlrel
      241 cadrqyayvt yhdlrsvpmy kdqaimavka ppeatlhvpq pinnlgqqkl qmhmrsshge
      301 ievflcpddp tvktspnpgy tttqpvpssk eseipdlppe llvnggsgvr vepvpsvest
      361 lntrsctpvv stvtslagmr dallcesddy gpmgggkfql qtedqintpd lsffefgehl
      421 lslepplsen dysfslgtee glsdlfdfkf
//
LOCUS       XP_034186043             450 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor E2F3 [Osmia lignaria lignaria].
ACCESSION   XP_034186043
VERSION     XP_034186043.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330152.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="transcription factor E2F3"
                     /calculated_mol_wt=50046
     Region          135..199
                     /region_name="E2F_TDP"
                     /note="E2F/DP family winged-helix DNA-binding domain;
                     pfam02319"
                     /db_xref="CDD:460530"
     Region          207..309
                     /region_name="E2F_DD"
                     /note="Dimerization domain of E2F transcription factors;
                     cd14660"
                     /db_xref="CDD:271137"
     Region          207..242
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:271137"
     Site            order(208..209,212..213,215..216,219..220,222..223,
                     226..227,229..230,232..234,236..237,240,245..250,253..254,
                     257,261..269,289..296,298..301,303,309)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:271137"
     Site            order(271..280,284..285,290)
                     /site_type="other"
                     /note="RbC binding site [polypeptide binding]"
                     /db_xref="CDD:271137"
     CDS             1..450
                     /gene="LOC117607003"
                     /coded_by="XM_034330152.2:1037..2389"
                     /db_xref="GeneID:117607003"
ORIGIN      
        1 mprvrrqvvl edparpvtpd mvetklmkte flddgsldev qaqkvaeetp sphlqdhqyg
       61 qtpcyqvtrk ptqppprteq isvqavkrrl nlemgatgps qsafkaprgk rrrsgsnslt
      121 ghtptksktv ertrydtsls lltkkfihlv essqdgvvdl nvaseklevq krriyditnv
      181 legigilekk sknniqwkgg qlpndrndia dlrrevadle akentldrli hgadknlrel
      241 cadrqyayvt yhdlrsvpmy kdqaimavka ppeatlhvpq pinnlgqqkl qmhmrsshge
      301 ievflcpddp tvktspnpgy tttqpvpssk eseipdlppe llvnggsgvr vepvpsvest
      361 lntrsctpvv stvtslagmr dallcesddy gpmgggkfql qtedqintpd lsffefgehl
      421 lslepplsen dysfslgtee glsdlfdfkf
//
LOCUS       XP_034186060             450 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor E2F3 [Osmia lignaria lignaria].
ACCESSION   XP_034186060
VERSION     XP_034186060.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330169.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="transcription factor E2F3"
                     /calculated_mol_wt=50046
     Region          135..199
                     /region_name="E2F_TDP"
                     /note="E2F/DP family winged-helix DNA-binding domain;
                     pfam02319"
                     /db_xref="CDD:460530"
     Region          207..309
                     /region_name="E2F_DD"
                     /note="Dimerization domain of E2F transcription factors;
                     cd14660"
                     /db_xref="CDD:271137"
     Region          207..242
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:271137"
     Site            order(208..209,212..213,215..216,219..220,222..223,
                     226..227,229..230,232..234,236..237,240,245..250,253..254,
                     257,261..269,289..296,298..301,303,309)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:271137"
     Site            order(271..280,284..285,290)
                     /site_type="other"
                     /note="RbC binding site [polypeptide binding]"
                     /db_xref="CDD:271137"
     CDS             1..450
                     /gene="LOC117607003"
                     /coded_by="XM_034330169.2:160..1512"
                     /db_xref="GeneID:117607003"
ORIGIN      
        1 mprvrrqvvl edparpvtpd mvetklmkte flddgsldev qaqkvaeetp sphlqdhqyg
       61 qtpcyqvtrk ptqppprteq isvqavkrrl nlemgatgps qsafkaprgk rrrsgsnslt
      121 ghtptksktv ertrydtsls lltkkfihlv essqdgvvdl nvaseklevq krriyditnv
      181 legigilekk sknniqwkgg qlpndrndia dlrrevadle akentldrli hgadknlrel
      241 cadrqyayvt yhdlrsvpmy kdqaimavka ppeatlhvpq pinnlgqqkl qmhmrsshge
      301 ievflcpddp tvktspnpgy tttqpvpssk eseipdlppe llvnggsgvr vepvpsvest
      361 lntrsctpvv stvtslagmr dallcesddy gpmgggkfql qtedqintpd lsffefgehl
      421 lslepplsen dysfslgtee glsdlfdfkf
//
LOCUS       XP_034186073             446 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 6-O-sulfotransferase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186073
VERSION     XP_034186073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="heparan sulfate 6-O-sulfotransferase isoform X1"
                     /calculated_mol_wt=51043
     Region          133..396
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; pfam03567"
                     /db_xref="CDD:427369"
     CDS             1..446
                     /gene="Hs6st"
                     /coded_by="XM_034330182.2:1..1341"
                     /db_xref="GeneID:117607018"
ORIGIN      
        1 mgrrsneegv selsvlvqdd lcertatmes rarrtrlrsv vgiclflalt siiylgyfcp
       61 dhvcaltsre ikesvrlseg lsagpgsgls svsvefdkng llsvhpafrl qsiqgslgyd
      121 dvftndtfqf dinghdvivf lhiqktggtl fgkhlvrdld lqrpcscqrr rkrcfcfrpn
      181 rnenwlfsry stgwkcglha dwteltgcvd telnkiegdg ikrryfyiti irdpvaryls
      241 efrhvqrgat wrgarhwcgg tqanipqcyp gsswqgvsle qfmacpynla snrqtrmlad
      301 lsivgcynst lsslerdrlm lasakhnlqf mpffmlteyq kvgqysfeet fgmrfavafe
      361 qhnatlsaat matltaeqld avrrlnrldl elyefaknla fqrfkrlrdr dpyfvqrfqh
      421 lgelpsrqsv tefnwdsvie dttdve
//
LOCUS       XP_034186082             386 aa            linear   INV 03-SEP-2025
DEFINITION  heparan sulfate 6-O-sulfotransferase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186082
VERSION     XP_034186082.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="heparan sulfate 6-O-sulfotransferase isoform X2"
                     /calculated_mol_wt=44092
     Region          133..>252
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; cl22970"
                     /db_xref="CDD:451469"
     CDS             1..386
                     /gene="Hs6st"
                     /coded_by="XM_034330191.2:1..1161"
                     /db_xref="GeneID:117607018"
ORIGIN      
        1 mgrrsneegv selsvlvqdd lcertatmes rarrtrlrsv vgiclflalt siiylgyfcp
       61 dhvcaltsre ikesvrlseg lsagpgsgls svsvefdkng llsvhpafrl qsiqgslgyd
      121 dvftndtfqf dinghdvivf lhiqktggtl fgkhlvrdld lqrpcscqrr rkrcfcfrpn
      181 rnenwlfsry stgwkcglha dwteltgcvd telnkiegdg ikrryfyiti irdpvaryls
      241 efrhvqrgat wrgarhwcgg tqanipqcyp gsswqgvsle qvgqysfeet fgmrfavafe
      301 qhnatlsaat matltaeqld avrrlnrldl elyefaknla fqrfkrlrdr dpyfvqrfqh
      361 lgelpsrqsv tefnwdsvie dttdve
//
LOCUS       XP_076546259             422 aa            linear   INV 03-SEP-2025
DEFINITION  elongation factor 1-delta isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546259
VERSION     XP_076546259.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690144.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="elongation factor 1-delta isoform X1"
                     /calculated_mol_wt=47062
     Region          300..322
                     /region_name="EF-1_beta_acid"
                     /note="Eukaryotic elongation factor 1 beta central acidic
                     region; pfam10587"
                     /db_xref="CDD:463158"
     Region          339..422
                     /region_name="EF1_GNE"
                     /note="EF-1 guanine nucleotide exchange domain; pfam00736"
                     /db_xref="CDD:459919"
     CDS             1..422
                     /gene="LOC117607032"
                     /coded_by="XM_076690144.1:201..1469"
                     /db_xref="GeneID:117607032"
ORIGIN      
        1 meksalaqek vwfdkpsydk aerlyferma kvvshkimes cslksevslt nncqdilnsn
       61 slsktkskks kdnqliisdn isnditnskk kmsntnkded eynmqkkenv eekqksvkfk
      121 ndtkgeetsp tndvskkynt kevkeeknka dtrhrnrgka kkdakesken vasltrnkqq
      181 lptqgnqqtt ssmlsaggsl anevakarqh ikqslqcmdd iaavagltap ndnkkdtvdv
      241 slvqslknii dkldervkal edkvsllyta pptlavcpak pepatkqtqe kadddedvdl
      301 fgsdseakda eaakireerl aayaakkskk paliaksnii ldvkpwddet dmkametkvr
      361 kiecdgllwg aaklvplafg ihklqiscvv eddkvsvdwl tekiqeiedf vqsvdiaafn
      421 kv
//
LOCUS       XP_076546260             422 aa            linear   INV 03-SEP-2025
DEFINITION  elongation factor 1-delta isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546260
VERSION     XP_076546260.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690145.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="elongation factor 1-delta isoform X1"
                     /calculated_mol_wt=47062
     Region          300..322
                     /region_name="EF-1_beta_acid"
                     /note="Eukaryotic elongation factor 1 beta central acidic
                     region; pfam10587"
                     /db_xref="CDD:463158"
     Region          339..422
                     /region_name="EF1_GNE"
                     /note="EF-1 guanine nucleotide exchange domain; pfam00736"
                     /db_xref="CDD:459919"
     CDS             1..422
                     /gene="LOC117607032"
                     /coded_by="XM_076690145.1:185..1453"
                     /db_xref="GeneID:117607032"
ORIGIN      
        1 meksalaqek vwfdkpsydk aerlyferma kvvshkimes cslksevslt nncqdilnsn
       61 slsktkskks kdnqliisdn isnditnskk kmsntnkded eynmqkkenv eekqksvkfk
      121 ndtkgeetsp tndvskkynt kevkeeknka dtrhrnrgka kkdakesken vasltrnkqq
      181 lptqgnqqtt ssmlsaggsl anevakarqh ikqslqcmdd iaavagltap ndnkkdtvdv
      241 slvqslknii dkldervkal edkvsllyta pptlavcpak pepatkqtqe kadddedvdl
      301 fgsdseakda eaakireerl aayaakkskk paliaksnii ldvkpwddet dmkametkvr
      361 kiecdgllwg aaklvplafg ihklqiscvv eddkvsvdwl tekiqeiedf vqsvdiaafn
      421 kv
//
LOCUS       XP_034186110             393 aa            linear   INV 03-SEP-2025
DEFINITION  protein artemis isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186110
VERSION     XP_034186110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="protein artemis isoform X2"
                     /calculated_mol_wt=45561
     Region          1..168
                     /region_name="metallo-hydrolase-like_MBL-fold"
                     /note="mainly hydrolytic enzymes and related proteins
                     which carry out various biological functions; MBL-fold
                     metallohydrolase domain; cl23716"
                     /db_xref="CDD:451500"
     Site            order(33,35,37..38,110..111,131,164)
                     /site_type="active"
                     /db_xref="CDD:293792"
     Region          242..336
                     /region_name="DRMBL"
                     /note="DNA repair metallo-beta-lactamase; pfam07522"
                     /db_xref="CDD:429512"
     CDS             1..393
                     /gene="LOC117607032"
                     /coded_by="XM_034330219.2:147..1328"
                     /db_xref="GeneID:117607032"
ORIGIN      
        1 mstflglike ipgisvdrfd genltssvff lshchydhmh gladhffeyl dennkylycs
       61 hitkallknk fkfkdncvke vtidtsivie ykvqnedeil isvtsisagh cpgsvmflfe
      121 rnnvsvlytg dfrinpkdfs klkslhyckd sklfpriftk iyldttflsn dfaffptrqe
      181 tvlkictvik dwlnkdprnv vilecsatyg seflfielsk vlnmkihvkn yvyetycrie
      241 qlsccvtndp nstpihackq kisssglhcr snvkkenimi iipsvmkwrk kdtsvigewd
      301 tvrertfnvc yathssfnel kafiqyfkal eiypcvvkte eeqevyhlld eianksdkqi
      361 ilkqtyklkl pnkevsnkvp fkseyfssdd dsl
//
LOCUS       XP_076546261             389 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607032 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546261
VERSION     XP_076546261.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690146.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="uncharacterized protein LOC117607032 isoform X3"
                     /calculated_mol_wt=43636
     Region          267..289
                     /region_name="EF-1_beta_acid"
                     /note="Eukaryotic elongation factor 1 beta central acidic
                     region; pfam10587"
                     /db_xref="CDD:463158"
     Region          306..389
                     /region_name="EF1_GNE"
                     /note="EF-1 guanine nucleotide exchange domain; pfam00736"
                     /db_xref="CDD:459919"
     CDS             1..389
                     /gene="LOC117607032"
                     /coded_by="XM_076690146.1:201..1370"
                     /db_xref="GeneID:117607032"
ORIGIN      
        1 meksalaqek vwfdkpsydk aerlyferma kvvshkimes cslksevslt nncqdilnsn
       61 slsktkskks kdnqliisdn isnditnskk kmsntnkded eynmqkkenv eekqksvkfk
      121 ndtkgeetsp tndvskkynt kevkeeknka dtrhrnrgka kkdakesken vasltrnkqq
      181 lptqmddiaa vagltapndn kkdtvdvslv qslkniidkl dervkaledk vsllytappt
      241 lavcpakpep atkqtqekad ddedvdlfgs dseakdaeaa kireerlaay aakkskkpal
      301 iaksniildv kpwddetdmk ametkvrkie cdgllwgaak lvplafgihk lqiscvvedd
      361 kvsvdwltek iqeiedfvqs vdiaafnkv
//
LOCUS       XP_076546262             269 aa            linear   INV 03-SEP-2025
DEFINITION  putative elongation factor 1-delta isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546262
VERSION     XP_076546262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690147.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="putative elongation factor 1-delta isoform X4"
                     /calculated_mol_wt=29598
     Region          147..169
                     /region_name="EF-1_beta_acid"
                     /note="Eukaryotic elongation factor 1 beta central acidic
                     region; pfam10587"
                     /db_xref="CDD:463158"
     Region          186..269
                     /region_name="EF1_GNE"
                     /note="EF-1 guanine nucleotide exchange domain; pfam00736"
                     /db_xref="CDD:459919"
     CDS             1..269
                     /gene="LOC117607032"
                     /coded_by="XM_076690147.1:198..1007"
                     /db_xref="GeneID:117607032"
ORIGIN      
        1 meksalaqek vwfdkpsydk aerlyferma kgnqqttssm lsaggslane vakarqhikq
       61 slqcmddiaa vagltapndn kkdtvdvslv qslkniidkl dervkaledk vsllytappt
      121 lavcpakpep atkqtqekad ddedvdlfgs dseakdaeaa kireerlaay aakkskkpal
      181 iaksniildv kpwddetdmk ametkvrkie cdgllwgaak lvplafgihk lqiscvvedd
      241 kvsvdwltek iqeiedfvqs vdiaafnkv
//
LOCUS       XP_034186144             352 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase PP1-beta catalytic subunit
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186144
VERSION     XP_034186144.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330253.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="serine/threonine-protein phosphatase PP1-beta
                     catalytic subunit isoform X1"
                     /calculated_mol_wt=40286
     Region          7..321
                     /region_name="MPP_PP1_PPKL"
                     /note="PP1, PPKL (PP1 and kelch-like) enzymes, and related
                     proteins, metallophosphatase domain; cd07414"
                     /db_xref="CDD:277359"
     Site            order(87,89,115,119,147..148,152..153,157,196,218..220,
                     229,243..245,271,295..299)
                     /site_type="active"
                     /db_xref="CDD:277359"
     Site            order(87,89,115,147,196,271)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277359"
     Site            order(119,152..153,157,218..220,229,243..245,295..299)
                     /site_type="other"
                     /note="natural toxin binding site [chemical binding]"
                     /db_xref="CDD:277359"
     Site            order(202,204,221,226,239,248,256..257,259..261,263..265,
                     280,284,311..312,314,316,318)
                     /site_type="other"
                     /note="MYPT1 binding site [polypeptide binding]"
                     /db_xref="CDD:277359"
     CDS             1..352
                     /gene="LOC117607047"
                     /coded_by="XM_034330253.2:416..1474"
                     /db_xref="GeneID:117607047"
ORIGIN      
        1 madvelnvdn liqrllevyh tqkdqvqkka klkdifssdf vvrgcrpgkt vvmsegevrg
       61 lclksreifl qqpillelea plkicgdihg qytdllrlfe yggfppeany lflgdyvdrg
      121 kqsleticll laykikypen ffllrgnhec asinriygfy deckrrynik lwktftdcfn
      181 clpiaaiide kifcchggls pdlqnmeqir rimrptdvpd tgllcdllws dpdkevqgwg
      241 endrgvsftf gpdvvskfln rhdmdlicra hqvvedgyef fakrqlvtlf sapnycgefd
      301 naggmmsvde tlmcsfqilk psekkakyqy lsmntgqatr pstpqrnpak kk
//
LOCUS       XP_034186161             328 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase PP1-beta catalytic subunit
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186161
VERSION     XP_034186161.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330270.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="serine/threonine-protein phosphatase PP1-beta
                     catalytic subunit isoform X2"
                     /calculated_mol_wt=37450
     Region          7..297
                     /region_name="MPP_PP1_PPKL"
                     /note="PP1, PPKL (PP1 and kelch-like) enzymes, and related
                     proteins, metallophosphatase domain; cd07414"
                     /db_xref="CDD:277359"
     Site            order(63,65,91,95,123..124,128..129,133,172,194..196,205,
                     219..221,247,271..275)
                     /site_type="active"
                     /db_xref="CDD:277359"
     Site            order(63,65,91,123,172,247)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277359"
     Site            order(95,128..129,133,194..196,205,219..221,271..275)
                     /site_type="other"
                     /note="natural toxin binding site [chemical binding]"
                     /db_xref="CDD:277359"
     Site            order(178,180,197,202,215,224,232..233,235..237,239..241,
                     256,260,287..288,290,292,294)
                     /site_type="other"
                     /note="MYPT1 binding site [polypeptide binding]"
                     /db_xref="CDD:277359"
     CDS             1..328
                     /gene="LOC117607047"
                     /coded_by="XM_034330270.2:414..1400"
                     /db_xref="GeneID:117607047"
ORIGIN      
        1 madvelnvdn liqrllevrg crpgktvvms egevrglclk sreiflqqpi lleleaplki
       61 cgdihgqytd llrlfeyggf ppeanylflg dyvdrgkqsl eticlllayk ikypenffll
      121 rgnhecasin riygfydeck rryniklwkt ftdcfnclpi aaiidekifc chgglspdlq
      181 nmeqirrimr ptdvpdtgll cdllwsdpdk evqgwgendr gvsftfgpdv vskflnrhdm
      241 dlicrahqvv edgyeffakr qlvtlfsapn ycgefdnagg mmsvdetlmc sfqilkpsek
      301 kakyqylsmn tgqatrpstp qrnpakkk
//
LOCUS       XP_076546263             300 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase PP1-beta catalytic subunit
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546263
VERSION     XP_076546263.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690148.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="serine/threonine-protein phosphatase PP1-beta
                     catalytic subunit isoform X3"
                     /calculated_mol_wt=34330
     Region          1..269
                     /region_name="MPP_PP1_PPKL"
                     /note="PP1, PPKL (PP1 and kelch-like) enzymes, and related
                     proteins, metallophosphatase domain; cd07414"
                     /db_xref="CDD:277359"
     Site            order(35,37,63,67,95..96,100..101,105,144,166..168,177,
                     191..193,219,243..247)
                     /site_type="active"
                     /db_xref="CDD:277359"
     Site            order(35,37,63,95,144,219)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277359"
     Site            order(67,100..101,105,166..168,177,191..193,243..247)
                     /site_type="other"
                     /note="natural toxin binding site [chemical binding]"
                     /db_xref="CDD:277359"
     Site            order(150,152,169,174,187,196,204..205,207..209,211..213,
                     228,232,259..260,262,264,266)
                     /site_type="other"
                     /note="MYPT1 binding site [polypeptide binding]"
                     /db_xref="CDD:277359"
     CDS             1..300
                     /gene="LOC117607047"
                     /coded_by="XM_076690148.1:551..1453"
                     /db_xref="GeneID:117607047"
ORIGIN      
        1 msegevrglc lksreiflqq pilleleapl kicgdihgqy tdllrlfeyg gfppeanylf
       61 lgdyvdrgkq sleticllla ykikypenff llrgnhecas inriygfyde ckrryniklw
      121 ktftdcfncl piaaiideki fcchgglspd lqnmeqirri mrptdvpdtg llcdllwsdp
      181 dkevqgwgen drgvsftfgp dvvskflnrh dmdlicrahq vvedgyeffa krqlvtlfsa
      241 pnycgefdna ggmmsvdetl mcsfqilkps ekkakyqyls mntgqatrps tpqrnpakkk
//
LOCUS       XP_034186166             300 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase PP1-beta catalytic subunit
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186166
VERSION     XP_034186166.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330275.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="serine/threonine-protein phosphatase PP1-beta
                     catalytic subunit isoform X3"
                     /calculated_mol_wt=34330
     Region          1..269
                     /region_name="MPP_PP1_PPKL"
                     /note="PP1, PPKL (PP1 and kelch-like) enzymes, and related
                     proteins, metallophosphatase domain; cd07414"
                     /db_xref="CDD:277359"
     Site            order(35,37,63,67,95..96,100..101,105,144,166..168,177,
                     191..193,219,243..247)
                     /site_type="active"
                     /db_xref="CDD:277359"
     Site            order(35,37,63,95,144,219)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277359"
     Site            order(67,100..101,105,166..168,177,191..193,243..247)
                     /site_type="other"
                     /note="natural toxin binding site [chemical binding]"
                     /db_xref="CDD:277359"
     Site            order(150,152,169,174,187,196,204..205,207..209,211..213,
                     228,232,259..260,262,264,266)
                     /site_type="other"
                     /note="MYPT1 binding site [polypeptide binding]"
                     /db_xref="CDD:277359"
     CDS             1..300
                     /gene="LOC117607047"
                     /coded_by="XM_034330275.2:170..1072"
                     /db_xref="GeneID:117607047"
ORIGIN      
        1 msegevrglc lksreiflqq pilleleapl kicgdihgqy tdllrlfeyg gfppeanylf
       61 lgdyvdrgkq sleticllla ykikypenff llrgnhecas inriygfyde ckrryniklw
      121 ktftdcfncl piaaiideki fcchgglspd lqnmeqirri mrptdvpdtg llcdllwsdp
      181 dkevqgwgen drgvsftfgp dvvskflnrh dmdlicrahq vvedgyeffa krqlvtlfsa
      241 pnycgefdna ggmmsvdetl mcsfqilkps ekkakyqyls mntgqatrps tpqrnpakkk
//
LOCUS       XP_076546264             345 aa            linear   INV 03-SEP-2025
DEFINITION  mortality factor 4-like isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546264
VERSION     XP_076546264.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690149.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..345
                     /product="mortality factor 4-like isoform X1"
                     /calculated_mol_wt=39443
     Region          20..77
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(26..27,31..34,50,52..55,59,61..64,66..68,71..72)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(31,52,55,59)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Site            order(49,51..56,58..62)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(49,52,56,58,62)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          160..333
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..345
                     /gene="MRG15"
                     /coded_by="XM_076690149.1:4302..5339"
                     /db_xref="GeneID:117607069"
ORIGIN      
        1 mratggfpsv vrrrlsgvge kvlcfhgpli yeakclkssi tkekqikyfi hyagwnknwd
       61 ewvpesrvlk yneanvqrqr evqrahsnqq saqktkkgst ssktqgrrse gvrekdtdsr
      121 astpvatvdk gvsrfskgts ssvtpssshd stseaprkkr srlepsgete eyltkvevki
      181 kipeelkfvl idesevilkh hklpalpvkn tvdkilddyv eskssgknds vrestleitk
      241 gireyfnisl glqllykwer pqfiqimndn petlpsqlyg afhllrlfvr lggmlsyttl
      301 dersiqllls hfhdflqylq knnaelfnlq qdyadsppdy hrkya
//
LOCUS       XP_034186176             336 aa            linear   INV 03-SEP-2025
DEFINITION  mortality factor 4-like isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186176
VERSION     XP_034186176.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330285.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="mortality factor 4-like isoform X2"
                     /calculated_mol_wt=38604
     Region          11..68
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(17..18,22..25,41,43..46,50,52..55,57..59,62..63)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(22,43,46,50)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Site            order(40,42..47,49..53)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(40,43,47,49,53)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          151..324
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..336
                     /gene="MRG15"
                     /coded_by="XM_034330285.2:344..1354"
                     /db_xref="GeneID:117607069"
ORIGIN      
        1 mppkckfqeg ekvlcfhgpl iyeakclkss itkekqikyf ihyagwnknw dewvpesrvl
       61 kyneanvqrq revqrahsnq qsaqktkkgs tssktqgrrs egvrekdtds rastpvatvd
      121 kgvsrfskgt sssvtpsssh dstseaprkk rsrlepsget eeyltkvevk ikipeelkfv
      181 lidesevilk hhklpalpvk ntvdkilddy veskssgknd svrestleit kgireyfnis
      241 lglqllykwe rpqfiqimnd npetlpsqly gafhllrlfv rlggmlsytt ldersiqlll
      301 shfhdflqyl qknnaelfnl qqdyadsppd yhrkya
//
LOCUS       XP_034186187             330 aa            linear   INV 03-SEP-2025
DEFINITION  mortality factor 4-like isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186187
VERSION     XP_034186187.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330296.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="mortality factor 4-like isoform X3"
                     /calculated_mol_wt=38050
     Region          5..62
                     /region_name="CBD_MSL3_like"
                     /note="chromo barrel domain of human male-specific lethal
                     complex subunit 3, and similar proteins; cd18983"
                     /db_xref="CDD:350846"
     Site            order(11..12,16..19,35,37..40,44,46..49,51..53,56..57)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:350846"
     Site            order(16,37,40,44)
                     /site_type="other"
                     /note="putative methylated histone tail binding site
                     [chemical binding]"
                     /db_xref="CDD:350846"
     Site            order(34,36..41,43..47)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Site            order(34,37,41,43,47)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:350846"
     Region          145..318
                     /region_name="MRG"
                     /note="pfam05712"
                     /db_xref="CDD:461721"
     CDS             1..330
                     /gene="MRG15"
                     /coded_by="XM_034330296.2:408..1400"
                     /db_xref="GeneID:117607069"
ORIGIN      
        1 mfqwekvlcf hgpliyeakc lkssitkekq ikyfihyagw nknwdewvpe srvlkynean
       61 vqrqrevqra hsnqqsaqkt kkgstssktq grrsegvrek dtdsrastpv atvdkgvsrf
      121 skgtsssvtp ssshdstsea prkkrsrlep sgeteeyltk vevkikipee lkfvlidese
      181 vilkhhklpa lpvkntvdki lddyveskss gkndsvrest leitkgirey fnislglqll
      241 ykwerpqfiq imndnpetlp sqlygafhll rlfvrlggml syttldersi qlllshfhdf
      301 lqylqknnae lfnlqqdyad sppdyhrkya
//
LOCUS       XP_034186217             294 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607075 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186217
VERSION     XP_034186217.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330326.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="uncharacterized protein LOC117607075 isoform X1"
                     /calculated_mol_wt=33629
     Region          180..236
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..294
                     /gene="LOC117607075"
                     /coded_by="XM_034330326.2:363..1247"
                     /db_xref="GeneID:117607075"
ORIGIN      
        1 mangydecyd aswhlfppdy vedeyriles ikmprtsfhi hdilqldskp sqeetevqgs
       61 ttvlpttndv psayqqffeh ttamlqpaiy anlnrgtlpp pppgllgwpt gptlpttlpq
      121 pleevnvlqq pdstsptisd lsfppkqetn hdckeeesad fedeqqtnet qphetehkkr
      181 krrvlfskaq tfelerrfrq qrylsapere hlasiirltp tqvkiwfqnh ryktkraate
      241 rveagasgcs prrvavplli kdgkpcqskl mepasypsag qvpmppymqk pyww
//
LOCUS       XP_034186227             301 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607075 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186227
VERSION     XP_034186227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330336.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..301
                     /product="uncharacterized protein LOC117607075 isoform X2"
                     /calculated_mol_wt=34374
     Region          187..243
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..301
                     /gene="LOC117607075"
                     /coded_by="XM_034330336.2:16..921"
                     /db_xref="GeneID:117607075"
ORIGIN      
        1 madknkilpw pllsyptsll nhvwysqlaf nnrilesikm prtsfhihdi lqldskpsqe
       61 etevqgsttv lpttndvpsa yqqffehtta mlqpaiyanl nrgtlppppp gllgwptgpt
      121 lpttlpqple evnvlqqpds tsptisdlsf ppkqetnhdc keeesadfed eqqtnetqph
      181 etehkkrkrr vlfskaqtfe lerrfrqqry lsaperehla siirltptqv kiwfqnhryk
      241 tkraaterve agasgcsprr vavpllikdg kpcqsklmep asypsagqvp mppymqkpyw
      301 w
//
LOCUS       XP_034186239             275 aa            linear   INV 03-SEP-2025
DEFINITION  myofilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186239
VERSION     XP_034186239.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330348.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="myofilin isoform X1"
                     /calculated_mol_wt=32748
     Region          5..145
                     /region_name="Myofilin"
                     /note="pfam15929"
                     /db_xref="CDD:374232"
     CDS             1..275
                     /gene="Mf"
                     /coded_by="XM_034330348.2:200..1027"
                     /db_xref="GeneID:117607085"
ORIGIN      
        1 mdarfrsnld migrnepitr kakfwqsyvr alkgtddmra pehthrprsi frsdypelhs
       61 tswpfgksif enplhaadrv nvpgyrylpl hrdiygyspr qiyphqykpv erftparpfd
      121 pekawtdhln rladidrlyp srspllsrhi spplstkrlf dihgnlvtdy dylpsfpsll
      181 rkrplfelle pspmapisaf trdpwwypaf apyipsyaar stpfflrdsy lspvkrsylw
      241 srhpirpfgs lyyyysqpvp rfhftrpwyn krtty
//
LOCUS       XP_076546265             275 aa            linear   INV 03-SEP-2025
DEFINITION  myofilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546265
VERSION     XP_076546265.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690150.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="myofilin isoform X1"
                     /calculated_mol_wt=32748
     Region          5..145
                     /region_name="Myofilin"
                     /note="pfam15929"
                     /db_xref="CDD:374232"
     CDS             1..275
                     /gene="Mf"
                     /coded_by="XM_076690150.1:170..997"
                     /db_xref="GeneID:117607085"
ORIGIN      
        1 mdarfrsnld migrnepitr kakfwqsyvr alkgtddmra pehthrprsi frsdypelhs
       61 tswpfgksif enplhaadrv nvpgyrylpl hrdiygyspr qiyphqykpv erftparpfd
      121 pekawtdhln rladidrlyp srspllsrhi spplstkrlf dihgnlvtdy dylpsfpsll
      181 rkrplfelle pspmapisaf trdpwwypaf apyipsyaar stpfflrdsy lspvkrsylw
      241 srhpirpfgs lyyyysqpvp rfhftrpwyn krtty
//
LOCUS       XP_034186252             252 aa            linear   INV 03-SEP-2025
DEFINITION  myofilin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186252
VERSION     XP_034186252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330361.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..252
                     /product="myofilin isoform X2"
                     /calculated_mol_wt=29717
     Region          5..145
                     /region_name="Myofilin"
                     /note="pfam15929"
                     /db_xref="CDD:374232"
     CDS             1..252
                     /gene="Mf"
                     /coded_by="XM_034330361.2:200..958"
                     /db_xref="GeneID:117607085"
ORIGIN      
        1 mdarfrsnld migrnepitr kakfwqsyvr alkgtddmra pehthrprsi frsdypelhs
       61 tswpfgksif enplhaadrv nvpgyrylpl hrdiygyspr qiyphqykpv erftparpfd
      121 pekawtdhln rladidrlyp srspllsrhi spplstkrlf dihgnlvtdy dylpsfpsll
      181 rkrplfelle pspmapisaf trdpwwypaf apyipsyaar stpfflrdsy lspvkrsylw
      241 srhpirpfah ay
//
LOCUS       XP_034186265             263 aa            linear   INV 03-SEP-2025
DEFINITION  protein takeout [Osmia lignaria lignaria].
ACCESSION   XP_034186265
VERSION     XP_034186265.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330374.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="protein takeout"
                     /calculated_mol_wt=29191
     Region          33..260
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..263
                     /gene="LOC117607104"
                     /coded_by="XM_034330374.2:375..1166"
                     /db_xref="GeneID:117607104"
ORIGIN      
        1 misgsriacv mlvgllagel vscqkndpvl nepdfetpey ilpcnrsdpk vescfqktln
       61 hlqpyllkgi peldlppiep liipelgmen gqgavrvral fsnitvigag nysimktrvd
      121 ipsyridlhl gfpkielqgr yevvgnvllf piqshgdfwa lfgdvlaiar iqgaeeirdg
      181 vrylkvakml vdfslgrarf rvvdelngen vigqamnqfl nqnakeiiee mrpaasasia
      241 khfkdflgka lvkiplkvwl hdt
//
LOCUS       XP_034186274             263 aa            linear   INV 03-SEP-2025
DEFINITION  protein takeout [Osmia lignaria lignaria].
ACCESSION   XP_034186274
VERSION     XP_034186274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330383.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="protein takeout"
                     /calculated_mol_wt=29191
     Region          33..260
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..263
                     /gene="LOC117607104"
                     /coded_by="XM_034330383.2:260..1051"
                     /db_xref="GeneID:117607104"
ORIGIN      
        1 misgsriacv mlvgllagel vscqkndpvl nepdfetpey ilpcnrsdpk vescfqktln
       61 hlqpyllkgi peldlppiep liipelgmen gqgavrvral fsnitvigag nysimktrvd
      121 ipsyridlhl gfpkielqgr yevvgnvllf piqshgdfwa lfgdvlaiar iqgaeeirdg
      181 vrylkvakml vdfslgrarf rvvdelngen vigqamnqfl nqnakeiiee mrpaasasia
      241 khfkdflgka lvkiplkvwl hdt
//
LOCUS       XP_034186283             263 aa            linear   INV 03-SEP-2025
DEFINITION  protein takeout [Osmia lignaria lignaria].
ACCESSION   XP_034186283
VERSION     XP_034186283.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330392.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="protein takeout"
                     /calculated_mol_wt=29191
     Region          33..260
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..263
                     /gene="LOC117607104"
                     /coded_by="XM_034330392.2:286..1077"
                     /db_xref="GeneID:117607104"
ORIGIN      
        1 misgsriacv mlvgllagel vscqkndpvl nepdfetpey ilpcnrsdpk vescfqktln
       61 hlqpyllkgi peldlppiep liipelgmen gqgavrvral fsnitvigag nysimktrvd
      121 ipsyridlhl gfpkielqgr yevvgnvllf piqshgdfwa lfgdvlaiar iqgaeeirdg
      181 vrylkvakml vdfslgrarf rvvdelngen vigqamnqfl nqnakeiiee mrpaasasia
      241 khfkdflgka lvkiplkvwl hdt
//
LOCUS       XP_076546266             257 aa            linear   INV 03-SEP-2025
DEFINITION  circadian clock-controlled protein daywake [Osmia lignaria
            lignaria].
ACCESSION   XP_076546266
VERSION     XP_076546266.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690151.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..257
                     /product="circadian clock-controlled protein daywake"
                     /calculated_mol_wt=27743
     Region          17..238
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..257
                     /gene="LOC117607160"
                     /coded_by="XM_076690151.1:172..945"
                     /db_xref="GeneID:117607160"
ORIGIN      
        1 mvrrkccscr mrmligllai atwtgitvae lppgvqscpr ttdltqygkc vleqlqainp
       61 ylangipslk lpaldplylp sltvdrnles lkikanmtki rvygatnyhi ddlkanphdl
      121 svtlkvelph vhvngdydvq grllllplng agsfkgnftd tevfvnaqgk eatdkngvqr
      181 ieidklvtki rvgdgniklk appshtlaad aaatffnsnp rlvldiaspi iedtaatvsr
      241 alaaralgal tkeellp
//
LOCUS       XP_034186296             254 aa            linear   INV 03-SEP-2025
DEFINITION  juvenile hormone binding protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034186296
VERSION     XP_034186296.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330405.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="juvenile hormone binding protein 2"
                     /calculated_mol_wt=28548
     Region          29..252
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..254
                     /gene="Jhbp2"
                     /coded_by="XM_034330405.2:87..851"
                     /db_xref="GeneID:117607116"
ORIGIN      
        1 mttrliglll cscsllinvq aigryrglef lepcsrrdqn vesclarsvn vltehfrhgl
       61 pqlgypevep iildelhisv gggpegyraq fknitargvs slritglrtr isedevqlql
      121 alsipkiraa akyrssgtll lvkasgagdy wgeyesvkak vfirarpfsy qgrdylrlqq
      181 lkmdfsvqni kmgvenirds naiiiaalnl fintnsqell kemkpdlrrk lvqvmstfve
      241 kifaqvpyda wltd
//
LOCUS       XP_076546267             252 aa            linear   INV 03-SEP-2025
DEFINITION  protein takeout isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546267
VERSION     XP_076546267.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690152.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..252
                     /product="protein takeout isoform X1"
                     /calculated_mol_wt=28119
     Region          14..250
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..252
                     /gene="LOC117607134"
                     /coded_by="XM_076690152.1:1..759"
                     /db_xref="GeneID:117607134"
ORIGIN      
        1 mniftcfffp ffsltaalfc evsavlppyi kpckksepdi necitnsiee lreklsvgip
       61 eleappiepl llkeirltrg pigarldvnl tnlkvsgpsr fkvrdlkadv envkftfkvs
      121 ferlnfegky qidarilllk lagegdltgn fdnydsdvvl kankvfrdnd vylnfegmki
      181 kirigkanlh fsnlfggdtv lgaasheiln snselvvdei tpvletslae lftnvankit
      241 ksftykelfp dg
//
LOCUS       XP_034186319             248 aa            linear   INV 03-SEP-2025
DEFINITION  protein takeout isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186319
VERSION     XP_034186319.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330428.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="protein takeout isoform X2"
                     /calculated_mol_wt=27510
     Region          9..246
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..248
                     /gene="LOC117607134"
                     /coded_by="XM_034330428.2:76..822"
                     /db_xref="GeneID:117607134"
ORIGIN      
        1 myallarvcl taalfcevsa vlppyikpck ksepdineci tnsieelrek lsvgipelea
       61 ppieplllke irltrgpiga rldvnltnlk vsgpsrfkvr dlkadvenvk ftfkvsferl
      121 nfegkyqida rilllklage gdltgnfdny dsdvvlkank vfrdndvyln fegmkikiri
      181 gkanlhfsnl fggdtvlgaa sheilnsnse lvvdeitpvl etslaelftn vankitksft
      241 ykelfpdg
//
LOCUS       XP_034186307             252 aa            linear   INV 03-SEP-2025
DEFINITION  protein takeout [Osmia lignaria lignaria].
ACCESSION   XP_034186307
VERSION     XP_034186307.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330416.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..252
                     /product="protein takeout"
                     /calculated_mol_wt=28642
     Region          17..251
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..252
                     /gene="LOC117607125"
                     /coded_by="XM_034330416.2:72..830"
                     /db_xref="GeneID:117607125"
ORIGIN      
        1 mkiqksltil flyiyssass pipeipsflk vchrndpqls dcikesinll kpylkngike
       61 lqvppceplh iknididqss gpiyvhakyk nisifggtnt ipksikldfd knrmrlklyi
      121 prlemitnyn ldgkimmlpi tgngvghgnf tdidaiitlq meryrnldtq lihqkvddiy
      181 vdfeigratv hlenlfdgda tmssamnlfl ndnwrtvvae ikpkleetig eliknfmnni
      241 fsvfpedsll pp
//
LOCUS       XP_034186333             247 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607137 [Osmia lignaria lignaria].
ACCESSION   XP_034186333
VERSION     XP_034186333.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330442.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..247
                     /product="uncharacterized protein LOC117607137"
                     /calculated_mol_wt=28549
     Region          24..247
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..247
                     /gene="LOC117607137"
                     /coded_by="XM_034330442.2:76..819"
                     /db_xref="GeneID:117607137"
ORIGIN      
        1 mlfrairtis fafavvalva adyqlpqgvk tckrdsddfa sclrlaiqea wptfieglpd
       61 fdippldpyy vdsysteykn gqlrgklsgt dvrtyglaka rflsvqpemt ddffrleidl
      121 elpkllldgn yegegsldtf kfggqgffnm smedvrcswd itghvvndrw viehfrllpk
      181 igkmrvyfsd lfhgneqlnr aalifineyw plifrgmlpk mmdlwdeflt eftnrffsri
      241 pfstvfp
//
LOCUS       XP_034186340             247 aa            linear   INV 03-SEP-2025
DEFINITION  circadian clock-controlled protein daywake [Osmia lignaria
            lignaria].
ACCESSION   XP_034186340
VERSION     XP_034186340.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330449.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..247
                     /product="circadian clock-controlled protein daywake"
                     /calculated_mol_wt=26810
     Region          9..245
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..247
                     /gene="LOC117607150"
                     /coded_by="XM_034330449.2:141..884"
                     /db_xref="GeneID:117607150"
ORIGIN      
        1 mrkivliglv vatilsttfa rdlpdflhvc krndpnlgdc iknsvenfkp hlhsglpeyn
       61 ipsleplflk elvattgasi rlklsnirvn gasnfavtrl kanvdtlrfv ieldlprlsi
      121 nsdyevdgkv lllriqgtgl lngnftdcka lvkiqaevtk gqdglnyvti vdlktkisvg
      181 sgdlklnnlf ggdpvlgeav ngainsnfda fidelkpale ngisetfiki angilsqfty
      241 evlfpls
//
LOCUS       XP_034186367             246 aa            linear   INV 03-SEP-2025
DEFINITION  protein takeout [Osmia lignaria lignaria].
ACCESSION   XP_034186367
VERSION     XP_034186367.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330476.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..246
                     /product="protein takeout"
                     /calculated_mol_wt=27390
     Region          8..245
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..246
                     /gene="LOC117607161"
                     /coded_by="XM_034330476.2:251..991"
                     /db_xref="GeneID:117607161"
ORIGIN      
        1 mfetswfcll lilsgvttls vdsnpeyvkq csrsdpklka clidalhhlr pylsvgipei
       61 elpsvepfrm deltlsltgg ingykvqlre lyvkgasnyt vediklgspf eavvrmpali
      121 ldahysssgv liilpasgng tfhakfddvk alvkgtvstk vkdeqtylnv enldvelvvk
      181 hvqmrvrkif nnnrilteat nlflrengqe vlkvmepqlk kklsalfagi vnqllrhvpv
      241 evfllp
//
LOCUS       XP_034186383             244 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607162 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186383
VERSION     XP_034186383.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330492.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186383.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..244
                     /product="uncharacterized protein LOC117607162 isoform X1"
                     /calculated_mol_wt=27844
     CDS             1..244
                     /gene="LOC117607162"
                     /coded_by="XM_034330492.2:229..963"
                     /db_xref="GeneID:117607162"
ORIGIN      
        1 mkclviflit lqfhyglfak leskenqtev dqqrsarvhr vseypegllt kytlgsseqf
       61 ssgtqkilqt fseklqstlp dlndliqrea qlfkelngil kelpreeank lknvdlaine
      121 mtdisssatf tksllrnanh lnnnllnspe eldiyhqdgq ddekqtksrs svsqiestnd
      181 velkavknia qyldnvliei qnhityvrgv feklcrkhhp ftrlngnnma kfasmnqpkk
      241 vsif
//
LOCUS       XP_034186391             240 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607162 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186391
VERSION     XP_034186391.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330500.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186391.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="uncharacterized protein LOC117607162 isoform X2"
                     /calculated_mol_wt=27371
     CDS             1..240
                     /gene="LOC117607162"
                     /coded_by="XM_034330500.2:239..961"
                     /db_xref="GeneID:117607162"
ORIGIN      
        1 mkclviflit lqfhyglfak leskevdqqr sarvhrvsey peglltkytl gsseqfssgt
       61 qkilqtfsek lqstlpdlnd liqreaqlfk elngilkelp reeanklknv dlainemtdi
      121 sssatftksl lrnanhlnnn llnspeeldi yhqdgqddek qtksrssvsq iestndvelk
      181 avkniaqyld nvlieiqnhi tyvrgvfekl crkhhpftrl ngnnmakfas mnqpkkvsif
//
LOCUS       XP_034186404             243 aa            linear   INV 03-SEP-2025
DEFINITION  juvenile hormone binding protein 16 [Osmia lignaria lignaria].
ACCESSION   XP_034186404
VERSION     XP_034186404.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330513.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="juvenile hormone binding protein 16"
                     /calculated_mol_wt=27432
     Region          6..237
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..243
                     /gene="Jhbp16"
                     /coded_by="XM_034330513.2:162..893"
                     /db_xref="GeneID:117607171"
ORIGIN      
        1 mrstfalflf ficgsfaaef edhfkdchpn vpgfdscire alnairpyfk tglpkynvlp
       61 fdpffarevs vtrgmpsfgf titlknvtes gwtaskvtkf vsdlpnykvv ytqsfpakfl
      121 agsyefkgnm fgssvsnkgk ftlalydlvq tttvtrrpgq kikvsvdvqt irdlklhitn
      181 llfgrqiled ildriineaw qpgfvvtrrl indlvstgft eifgnafrnf pfekiikpkp
      241 das
//
LOCUS       XP_034186420             242 aa            linear   INV 03-SEP-2025
DEFINITION  putative beta-carotene-binding protein [Osmia lignaria lignaria].
ACCESSION   XP_034186420
VERSION     XP_034186420.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330529.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="putative beta-carotene-binding protein"
                     /calculated_mol_wt=27063
     Region          6..239
                     /region_name="JHBP"
                     /note="Haemolymph juvenile hormone binding protein (JHBP);
                     pfam06585"
                     /db_xref="CDD:461956"
     CDS             1..242
                     /gene="LOC117607176"
                     /coded_by="XM_034330529.2:120..848"
                     /db_xref="GeneID:117607176"
ORIGIN      
        1 mfsnqllflm tvigmavaev psyihvcgrk dpkldecvin svkslrdklr dgipeldipp
       61 veplkmskve lidtpsfkat atdiilrgll tyhvnflhvd lkkqqididv yfeenkldah
      121 fnitaqilvp ivgkgpislv aknvaakanl nfklvdhagk kyvffpsmtt nldvknydlk
      181 feaenfdnsl hdaisqalgn srqeildavk pnlekviset vlsmankigk hftydellpd
      241 re
//
LOCUS       XP_034186433             196 aa            linear   INV 03-SEP-2025
DEFINITION  SPRY domain-containing protein 7 [Osmia lignaria lignaria].
ACCESSION   XP_034186433
VERSION     XP_034186433.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330542.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="SPRY domain-containing protein 7"
                     /calculated_mol_wt=21326
     Region          37..196
                     /region_name="SPRYD7"
                     /note="SPRY domain-containing protein 7; cd12880"
                     /db_xref="CDD:293938"
     CDS             1..196
                     /gene="LOC117607181"
                     /coded_by="XM_034330542.2:31..621"
                     /db_xref="GeneID:117607181"
ORIGIN      
        1 mtmmfcclrn cfdglgfaat qmpkkepnpi aldtsfmghe vvivknglrv cgrggaltna
       61 plaqsksyfe vkiqqggiwa iglatrstdl nvaiggndte swalnsdgii rhnqqeihki
      121 qnqvqegdii gvsydhieln fylngksmda pimgikgtvy pvlyvddgai ldlildnfah
      181 ppptgfekim leqsll
//
LOCUS       XP_034186447             188 aa            linear   INV 03-SEP-2025
DEFINITION  dynactin subunit 3 [Osmia lignaria lignaria].
ACCESSION   XP_034186447
VERSION     XP_034186447.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330556.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186447.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..188
                     /product="dynactin subunit 3"
                     /calculated_mol_wt=21062
     Region          7..172
                     /region_name="Dynactin_p22"
                     /note="Dynactin subunit p22; pfam07426"
                     /db_xref="CDD:462162"
     CDS             1..188
                     /gene="LOC117607186"
                     /coded_by="XM_034330556.2:158..724"
                     /db_xref="GeneID:117607186"
ORIGIN      
        1 mgtielledr iahlekqiyg lmkanqgndm ppenpmtdql snvntlissa lsgrekanam
       61 mkrlpelnsy ldpefesvei pieaklqlll amapeiqrny ellqqmqelm pvletnclrd
      121 vpelsnklsn lnlsylklye esqglnnhin eifskynevi tsisesliai datvtaaeia
      181 ampkkqld
//
LOCUS       XP_034186456             188 aa            linear   INV 03-SEP-2025
DEFINITION  dynactin subunit 3 [Osmia lignaria lignaria].
ACCESSION   XP_034186456
VERSION     XP_034186456.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330565.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186456.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..188
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..188
                     /product="dynactin subunit 3"
                     /calculated_mol_wt=21062
     Region          7..172
                     /region_name="Dynactin_p22"
                     /note="Dynactin subunit p22; pfam07426"
                     /db_xref="CDD:462162"
     CDS             1..188
                     /gene="LOC117607186"
                     /coded_by="XM_034330565.2:162..728"
                     /db_xref="GeneID:117607186"
ORIGIN      
        1 mgtielledr iahlekqiyg lmkanqgndm ppenpmtdql snvntlissa lsgrekanam
       61 mkrlpelnsy ldpefesvei pieaklqlll amapeiqrny ellqqmqelm pvletnclrd
      121 vpelsnklsn lnlsylklye esqglnnhin eifskynevi tsisesliai datvtaaeia
      181 ampkkqld
//
LOCUS       XP_034186479             155 aa            linear   INV 03-SEP-2025
DEFINITION  protein archease [Osmia lignaria lignaria].
ACCESSION   XP_034186479
VERSION     XP_034186479.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330588.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="protein archease"
                     /calculated_mol_wt=17777
     Region          16..155
                     /region_name="Archease"
                     /note="Archease protein family (MTH1598/TM1083);
                     pfam01951"
                     /db_xref="CDD:426527"
     CDS             1..155
                     /gene="Archease"
                     /coded_by="XM_034330588.2:181..648"
                     /db_xref="GeneID:117607199"
ORIGIN      
        1 mdalkeedfv ippvkyeyld htadvqlhaw gdtmeeafeq camamfglmt dlarvqikqv
       61 hkveaeghdm esllfhflde llfmfsaepy ivakkvdivt fdrenfkita vaygeeftig
      121 khtqnaevka itysamqild rprterpeif vivdi
//
LOCUS       XP_034186503             125 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607206 [Osmia lignaria lignaria].
ACCESSION   XP_034186503
VERSION     XP_034186503.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330612.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="uncharacterized protein LOC117607206"
                     /calculated_mol_wt=12307
     CDS             1..125
                     /gene="LOC117607206"
                     /coded_by="XM_034330612.2:81..458"
                     /db_xref="GeneID:117607206"
ORIGIN      
        1 mgkfvliafa ailaiafayp aseqetslpr mareakadph hggvgglggv ggfgiiggfk
       61 vggfggyghg ypyrgyghgy gghgygghgh ghgyggypsy gggygvpygs asyasaqags
      121 igfgr
//
LOCUS       XP_034186518             125 aa            linear   INV 03-SEP-2025
DEFINITION  splicing factor 3B subunit 6 [Osmia lignaria lignaria].
ACCESSION   XP_034186518
VERSION     XP_034186518.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330627.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="splicing factor 3B subunit 6"
                     /calculated_mol_wt=14582
     Region          18..94
                     /region_name="RRM_SF3B14"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     branch site protein p14 (SF3B14) and similar proteins;
                     cd12241"
                     /db_xref="CDD:409687"
     Site            order(18,20..21,23,33,47..54,57,60,62,66..68,93)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:409687"
     Site            order(23,62,92..94)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:409687"
     CDS             1..125
                     /gene="Sf3b6"
                     /coded_by="XM_034330627.2:131..508"
                     /db_xref="GeneID:117607208"
ORIGIN      
        1 mtmamlqrra nvrlppevnr vlyirnlpyk itaeemydif gkygairqir vgntaetrgt
       61 afvvyedifd aknacdhlsg fnvcnrylvv lyyqsnkafk rvdvdkkmee idklktkynl
      121 neekk
//
LOCUS       XP_034172344             103 aa            linear   INV 03-SEP-2025
DEFINITION  histone H4 [Osmia lignaria lignaria].
ACCESSION   XP_034172344
VERSION     XP_034172344.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316453.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..103
                     /product="histone H4"
                     /calculated_mol_wt=11236
     Region          1..103
                     /region_name="PLN00035"
                     /note="histone H4; Provisional"
                     /db_xref="CDD:177669"
     CDS             1..103
                     /gene="LOC117600679"
                     /coded_by="XM_034316453.2:37..348"
                     /db_xref="GeneID:117600679"
ORIGIN      
        1 msgrgkggkg lgkggakrhr kvlrdniqgi tkpairrlar rggvkrisgl iyeetrgvlk
       61 vflenvirda vtytehakrk tvtamdvvya lkrqgrtlyg fgg
//
LOCUS       XP_076546269             175 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305669 [Osmia lignaria lignaria].
ACCESSION   XP_076546269
VERSION     XP_076546269.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690154.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="uncharacterized protein LOC143305669"
                     /calculated_mol_wt=20185
     Region          141..>175
                     /region_name="Transposase_1"
                     /note="Transposase (partial DDE domain); pfam01359"
                     /db_xref="CDD:460174"
     CDS             1..175
                     /gene="LOC143305669"
                     /coded_by="XM_076690154.1:1..528"
                     /db_xref="GeneID:143305669"
ORIGIN      
        1 mgiivdvpkp dfensndvef krgrttlqdd srsgcpktat teeiitkvhn ivlqdrrmkv
       61 seiakdmgis dervhhiltk elgmkklsar wvprlltdce nqkhirmqis qdcldrfkkn
      121 ttnfirrfvt tdetwihhyt pkskqqskqw khsdspppkk aksilsakkv masvf
//
LOCUS       XP_034192564             343 aa            linear   INV 03-SEP-2025
DEFINITION  histone-lysine N-methyltransferase SETMAR-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034192564
VERSION     XP_034192564.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336673.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192564.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..343
                     /product="histone-lysine N-methyltransferase SETMAR-like"
                     /calculated_mol_wt=39417
     Region          164..301
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     CDS             1..343
                     /gene="LOC117609914"
                     /coded_by="XM_034336673.2:142..1173"
                     /db_xref="GeneID:117609914"
ORIGIN      
        1 mlrsvseyyn mlfiyaqcng naelaaeryr etygvtanap iapcfqrlat rvyatgslml
       61 dridtgrstr ssasqeelvl aefeqddtts treisrrtnv spsnvykyvp vrnlrpqdfi
      121 rrvrysrwll gkiehnpsfc tyvlwtdeal fthegcftth tahvwsdedl hmilpraaqh
      181 rwsvnvwagi cgdfvvgpyi lpdkldgpay nqylqhvlpn llevipleir knmyyqhdea
      241 pahyaanvra hlnetfqdrw igrggpvawp pqspdvnpvd fylwghlrne vyrqhiksqe
      301 eavarihavv ktisvetlrk vqrnvasraa tcfrnqggfi ell
//
LOCUS       XP_034173466             874 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha9 subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034173466
VERSION     XP_034173466.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317575.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173466.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..874
                     /product="nicotinic acetylcholine receptor alpha9 subunit"
                     /calculated_mol_wt=100902
     Region          66..247
                     /region_name="LGIC_ECD_cation"
                     /note="extracellular domain (LBD) of cationic Cys-loop
                     neurotransmitter-gated ion channels; cd18989"
                     /db_xref="CDD:349790"
     Site            order(74,76,82..84,86,90,112,114,116,124,126,128,142,144,
                     146,156,158,160,164,166,186,192,212,247)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349790"
     Region          165..179
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349790"
     Region          501..685
                     /region_name="LGIC_ECD_cation"
                     /note="extracellular domain (LBD) of cationic Cys-loop
                     neurotransmitter-gated ion channels; cd18989"
                     /db_xref="CDD:349790"
     Site            order(511,512,518..520,522,526,548,550,552,560,562,564,
                     577,579,581,591,593,595,599,601,621,627,651)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349790"
     Region          600..614
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349790"
     CDS             1..874
                     /gene="nAChRa9"
                     /coded_by="XM_034317575.2:74..2698"
                     /db_xref="GeneID:117601156"
ORIGIN      
        1 mrtftalgll lvffhdgsea sldldlfsls cknydnpspm lnlkrhlfcq ydrtirptls
       61 hqivnnvtmk llpkilefde wnsrltlhsw maltwtdshl nwkpsdfdgi nfihvksdei
      121 wvpdlsvyns gdmaidqtgi psttclifss gsvscvpslr htakcstnfa hwpydthhcr
      181 infgswshsg eevnfnldkk afqmdgytnn sqwdfkvtda ykvlkkykcc pndtypmivy
      241 elyisrhygm lhttyvtpai vvmlltitvl wldsrsieri alasvnlich ilcifdmhwq
      301 lphngtnppy illyyrdsla mavfalvlta llrkmqdmsi dvpnwisttt tfvlnnragr
      361 fliltddngk lsnsgilgge neensdlpkt earskesswr lfaaiiewis ffavvftyii
      421 ilatlvlaty echidwcfrm ksifipflvi fcilqdhqgs cmflqsckei vdnstlqrlk
      481 eylfcfydee vrptddhrna tvvnfglyih qfevnelant vdfhvwsrmi wtnphltwep
      541 sqfdgitslq vmsyemwvpd ivlhstsnvd vdteipkvec wlyhlgtvic vptamyttfc
      601 esdhtwwpyd smncslyias wshsaneidi yfvtfdpegq lteeiydenl ewdlvhisqt
      661 erlveskfgv eftskmlsyh iilqryssty tssymtmvla lmtmtlmvlw ldprstermv
      721 vanfnfilhl iclqdlqwkl pyngnyppkl iffyenslvl aafsliltsi lrhvqelntd
      781 apawlssvti silkskvgqv flisildpkv sasmerntdd ntnlvsfdrk estwkytsil
      841 igwiaflsvl fvyvimlavf lptsnsaktf vvnt
//
LOCUS       XP_076546270            4994 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606165 [Osmia lignaria lignaria].
ACCESSION   XP_076546270
VERSION     XP_076546270.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690155.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4994
                     /product="uncharacterized protein LOC117606165"
                     /calculated_mol_wt=562143
     Region          863..912
                     /region_name="Ank_4"
                     /note="Ankyrin repeats (many copies); pfam13637"
                     /db_xref="CDD:372654"
     Region          893..922
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          908..1196
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          924..956
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          961..989
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1026..1056
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1027,1031..1032,1035..1037,1039..1040,1044,1047,
                     1056,1058,1060,1064..1065,1068..1070,1072..1073,1077,1080,
                     1089,1091,1093,1097..1098,1101..1103,1105..1106,1110,1113,
                     1122)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1058..1089
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1078..1361
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1091..1122
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1124..1154
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1158,1160,1164..1165,1168..1170,1172..1173,1177,
                     1180,1189,1191,1193,1197..1198,1201..1203,1205..1206,1210,
                     1213,1222,1224,1226,1230..1231,1234..1236,1238..1239,1243,
                     1246,1255)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1158..1189
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1191..1222
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1224..1255
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1257..1288
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1290..1320
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1323..1351
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1337..1616
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1356..1387
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1389..1420
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1422..1451
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1453..1482
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1488..1516
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1491..1492,1495..1497,1499..1500,1504,1507,1516,
                     1518,1520,1524..1525,1528..1530,1532..1533,1537,1540,1549,
                     1551,1556..1557,1561..1563,1565..1566,1570,1573,1582)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1500..1760
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1518..1549
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1551..1582
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1584..1617
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1619,1621,1625..1626,1629..1631,1633..1634,1638,
                     1641,1650,1652,1654,1658..1659,1662..1664,1666..1667,1671,
                     1674,1683,1685,1687,1691..1692,1695..1697,1699..1700,1704,
                     1707,1716)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1619..1650
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1632..>1924
                     /region_name="PHA03100"
                     /note="ankyrin repeat protein; Provisional; cl39094"
                     /db_xref="CDD:476869"
     Region          1652..1683
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1685..1716
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1718..1802
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1804..1835
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1839..1868
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <1971..2250
                     /region_name="FxSxx_TPR"
                     /note="FxSxx-COOH system tetratricopeptide repeat protein;
                     NF040586"
                     /db_xref="CDD:468560"
     Region          1997..2027
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          2086..2114
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          2126..2152
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(2128..2129,2132..2133,2135,2167,2170..2171,
                     2174..2175,2177..2178,2209,2212..2213,2216..2217,2220)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          2165..2195
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          2208..2236
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <2283..2446
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(2283,2286..2288,2290..2291,2295,2298,2307,2309,2311,
                     2315..2316,2319..2321,2323..2324,2328,2331,2340,2342,2344,
                     2348..2349,2352..2354,2356..2357,2361,2364)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          2309..2340
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2381..2413
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3361..3410
                     /region_name="Ank_4"
                     /note="Ankyrin repeats (many copies); pfam13637"
                     /db_xref="CDD:372654"
     Region          3391..3420
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3404..3694
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          3422..3454
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(3523,3525,3529..3530,3533..3535,3537..3538,3542,
                     3545,3554,3556,3558,3562..3563,3566..3568,3570..3571,3575,
                     3578,3587,3589,3591,3595..3596,3599..3601,3603..3604,3608,
                     3611,3620)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          3523..3554
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3556..3587
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3571..3859
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          3589..3620
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(3656,3658,3662..3663,3666..3668,3670..3671,3675,
                     3678,3687,3689,3691,3695..3696,3699..3701,3703..3704,3708,
                     3711,3720,3722,3724,3728..3729,3732..3734,3736..3737,3741,
                     3744,3753)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          3656..3687
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3689..3720
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3722..3753
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3755..3786
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3788..3818
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3803..4087
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(3821,3823,3827..3828,3831..3833,3835..3836,3840,
                     3843,3852,3854,3856,3860..3861,3864..3866,3868..3869,3873,
                     3876,3885,3887,3889,3893..3894,3897..3899,3901..3902,3906,
                     3909,3918)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          3821..3852
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3854..3885
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3887..3918
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3920..3949
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3954..3981
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          3986..4014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(3989..3990,3993..3995,3997..3998,4002,4005,4014,
                     4016,4018,4022..4023,4026..4028,4030..4031,4035,4038,4047,
                     4049,4054..4055,4058..4060,4063..4064,4068,4071,4080)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          3998..4253
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          4016..4047
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4049..4080
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4082..4114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(4116,4118,4122..4123,4126..4128,4130..4131,4135,
                     4138,4147,4149,4151,4155..4156,4159..4161,4163..4164,4168,
                     4171,4180,4182,4184,4188..4189,4192..4194,4196..4197,4201,
                     4204,4213)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          4116..4147
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4129..>4421
                     /region_name="PHA03100"
                     /note="ankyrin repeat protein; Provisional; cl39094"
                     /db_xref="CDD:476869"
     Region          4149..4180
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4182..4213
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4215..4302
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4304..4332
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4336..4365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <4468..4747
                     /region_name="FxSxx_TPR"
                     /note="FxSxx-COOH system tetratricopeptide repeat protein;
                     NF040586"
                     /db_xref="CDD:468560"
     Region          4494..4524
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          4583..4611
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          4623..4649
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(4625..4626,4629..4630,4632,4664,4667..4668,
                     4671..4672,4674..4675,4706,4709..4710,4713..4714,4717)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          4662..4692
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          4705..4732
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <4755..4943
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(4780,4783..4785,4787..4788,4792,4795,4804,4806,4808,
                     4812..4813,4816..4818,4820..4821,4825,4828,4837,4839,4841,
                     4845..4846,4849..4851,4853..4854,4858,4861)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          4806..4837
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4878..4910
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          4912..4966
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..4994
                     /gene="LOC117606165"
                     /coded_by="XM_076690155.1:1477..16461"
                     /db_xref="GeneID:117606165"
ORIGIN      
        1 meavnvskqq sssyqkhkkf llrsfatkge fvkeykklyn ekakteelfl mlfielektt
       61 lagdinslkk lnhlinyire iedqtalqdy ynntnnpikn tnlvilacke nkqeilkyll
      121 dnkiltnlsm nikesvilpd yedeashnaf yyairsgnve lldtlihkwp gnyftshlee
      181 ldeilsraye elklknipls dkieifvesk linlrffsnt skqgksyrgd lnnikeriel
      241 iiqnisllkt eyidaekvdt kflfiakfia qnihilkqql kstyniipwe emefclicfv
      301 ssyikqqein lfyfaildkr kilnqlqnfg kkleevknni ntmdiskfsd lpklkrqivv
      361 asiisnspef eelytdyqqi rdmysldkmn dyvqlalaid ptgregqlii trvlqvigey
      421 fkntlespkl snttseffll slpkytrevv idlrnsfsha yslskrteie kntdvnffig
      481 iqndirkfge vvtnifygnk ikmirillqk lidsqnvdei keiigelsti kfdkmirenc
      541 niteleklee lieklsdnvi ksiyekqlfd einniihlrn sestrtrtdy vtgfitlqsl
      601 svtcsdtein hnmvrgikff annilqyitp qiksrslkei aklvikiahs vksriqdemy
      661 ileiyriiyk ivgiiefein dikwieslre klhvtgtfip mykqrrtdnk iseiehnnql
      721 elklselqni lntnalneql idksssykqn eklqvvikml vldimsilgs lknyleddlf
      781 fldyntpllt gkclrnylah dnalvhilll dpsisvilna tklttenilk kkkkigklmk
      841 edisklkdky gyclaivsnq kqmfvaleeg nlenlkdcik kgadihardn nmwttvhfaa
      901 kgsslevvkf ilnqnvslnv kningqsplh iaalygrani vkffveetel ciddrdnnkk
      961 tplhtaaeng nkntveillm nganintqdl isyppslsav rnnhidvvkv llknevdvdf
     1021 tqsvggfsml hvaaefghqe ivdfllkkga nvnarndrgg iplhtaalng hlevvntlil
     1081 ngadvnarvl dgctslhyai enghekiani llehgananv sdktynntpl hyaamngher
     1141 iveallenna ntnittvegf tplhlavqng hleivttllk hstninvtak rkemllhyaa
     1201 ekgykeiael likngaevna iddtdltplh laalrghkdv idllienkak vniqningct
     1261 plhaavengn kdicdllikn kakvnaknnc gvtplhtaaa kgdkniidll iknkaevnak
     1321 cddgttplnv aatcghednv illiknaaiv nnkgifgftp lhsaimggyk nvvnllienn
     1381 aeinavgvgd ntplhlavei enkglveili rnganvnakn gnnmtplcca ikknskeive
     1441 vlvvngadvn vnegeplsla vfagykdiin vllqnnarid ikfrqsmsal htaaerghte
     1501 iantlianga dvnvttaisi tplhlaadgc hkemvellls kganvnaqsm dgtplhlavt
     1561 rdenvdvvrv llkngadvsi rdpknrrvle qavaykqldc vkalllhnkk ininakcndd
     1621 wtilhiaaqa gnlemvkyli negcdvnarn aagskpihia aregfkdivk fflskdcsid
     1681 dlgaanqtll hyaamkgqve avkhliaega diniqdrtgl splhiaanng yekvveille
     1741 ngsvyntvdk fsrtpreman sekvivqlss teklfeavkr nnssevekci rmgafvnakh
     1801 vgakgyvgtp lhfaawkgyd eiinillknk anpnatgnka ftplhyaakf shlkivmill
     1861 sngaiynais ddektpfhfa adknvndllk lvgesfrnvr ngnskviyel nkiknidkik
     1921 aimsasdrek qslivaamhs sfpkieqlkl igqgdistqi daallfanqe nynmalrfit
     1981 nalerrteil gpynpatlei qtdiakilyk qgsfqvavel ldeiyqkqke tlglyhkdtl
     2041 ntrstfalii hrqgkndeal diyqeiykkq kellgprhed tmntqfhmal vldkqekyee
     2101 alemnkdvfq krkeifganh rvtisaqnni amvlgnqgky eealkmykiv lekkrtvlgk
     2161 nhsdtlrtlf nmaglhlkqk kydeslkahq elfdiqktif geshpdtlnt qynlantyfw
     2221 qgkfisalki yseclekrsa vfgpthptvl nikqhielin drfkmegsga aeiigylqke
     2281 iniaaskgdv qtirnllrng advndkdvdg rvplhyavgn gytdvvkill engadvtqvt
     2341 nkgntplhta tsksykeive lllkhvsrdk lndfinaktt tsgtaslhia akngsldivk
     2401 slltygasyn iknkegkapi dvsnnqdins lfrlieklfk dtkngnvkvi eniqalkpde
     2461 flavtnarnn lrstllqvai fnkreyissn llklmgnime aanvskqqss scqehkkfll
     2521 rsfaskeefv reykklynek akteelflml fielekatla gdinslkkln ylidhirete
     2581 dqtalqdyyn ntnnpikntn lvilackenk qeilkylldn kiltnlsini desvilpdye
     2641 devshnafyy airsgniell dtliqkwpgn yftshleeld eilsrayeel klknvplsdk
     2701 ieifveskli nlrffsntsk qgksygcdln nikerielil qninllksey idtekvdtkf
     2761 lfiakfiaqn ihilkqqlks tyniipweei efclvcfvss yikqqeinlf yfailnkrki
     2821 lnqlqnfgkk leelknnint mdiskisdlp klkreivvas iisnspefee lytdyqqird
     2881 mysldkinny vqlalsidpa tregqliitr vlqvigeylq ntlespklsn tmsallllsl
     2941 pkytrevvid lhnsfshays lskrteiekn tdvnfcidiq idireigevi tniffgnkvk
     3001 virillqnii dsenvdeiqe iigelsnief dtmirenyni meleeleeli eelsntvmqt
     3061 nyeeelfdei nnilhlante stskktdyvv gfvtlrsfgf iysnteinhn fvrvyklfan
     3121 kilqsiiski nsrslkeiae liikiahcvk lrmqdemylt eiyriifkiv giiefeindi
     3181 kwieslrekl svkgslipmy kqnrtnnkis eiehnnqlel klselqniln tnalndqlid
     3241 klsfykqnkk lqvvvkmlvl dimsilgssk nyleddlffl dystplltgk clhnylahdn
     3301 alvhillpdp sisvilnark lttenilkkk kkigkliked isklkdkygh claivtnqkr
     3361 mfvaleegnl enlkdcikkg vdihardnnm wttihfaakg pslavvkfil nqnvslnvkn
     3421 ingqsplhia alygranivk ffveetelci ddrdnnkktp lhiaaengnr niveillmtg
     3481 aniitqdris fppllsavtn nhidvvkvll ekevdvdftq amdsctllhv aaecghleiv
     3541 efllkkganv narsdtrgtp lhtaalhghl eivnililng advnvrvldg stplhyaien
     3601 gyekianill ehgananvsd ktynntplhy aakggneriv eallnnnant niatvegftp
     3661 lhlavqnghl eivvtllkys ininvtvehk etllhyaaek gykeiaelli kngaevnaqd
     3721 nsgitplhla alrghkdvid lliknkakvn ikditnctpl yvatkngnkd icdlliknks
     3781 evnaknycgi tplhaaatkg dkniidllik nkakvnaecd dgttplniaa ihghednvil
     3841 likngakvnn rgilgftplq savmggykdv vnllikynak vnatciagnt plhlaveien
     3901 kelveilitn ganvnakngd nmtplccaik enskeivevl iangadvnvn egqplslavs
     3961 agdediinvl lqnsahidik frqtmsalht aaesghteia naliaqgadv nvttaisitp
     4021 lhlaaagchk emvelllskg anvnakcmdg tplhlavtkd envdvvrvll knaadvsikd
     4081 sknrtaleqa vahkqlecvk alllhkkini nakciddwtv lhiaaqagnl emikylineg
     4141 cdvnarntvg skpihiaare gfkdivkfll ikgcsihdlg idnqtllhya agkgqmevvr
     4201 yliaegadin iqdrngrspl hlaayydqee vveillenga vyntpdklir tpievtnsei
     4261 iifqllstek lfeavkrkns sevekcirmg afvnakhaga kgyqgtplhy aawkgydkii
     4321 nillenkanp natgnkaftp lhyaakfshl kivmillsng aiynaisdde ktpfhfaadk
     4381 nindllklvd esfrnvrngn skviyelnki knidkikaim sardrekqsl ivaamhsnfp
     4441 kieqlkvigq gdistqigaa llflnqenyd malrfftnai etrkeilgpy npatleilty
     4501 iakilykqga yevavelqde iyqkqketlg lyhkdtlntr sscaliihrq gkddealdiy
     4561 qevykkqkel lgprhedtlt tqfhmamvld kqekyeeale mnkdvfqkrk eiyganhhat
     4621 icaqnniamv lgnqgkyeea lkmyeivlek krtvlgknhs dtlrtlfnma glhlkqkkyd
     4681 eslkahqelf diqktifgen hpdtlntqyn lantyfclgk yisahkiyse clgkrsavfg
     4741 pthptvlnii qqirlinlrf eiggsgaaei teylqkeini aaskgdvqti rnllrngadv
     4801 ndrdvdgrtp lhyavsngha dvvkflleng advtqvtnkg ntplhtatsk nykelvelll
     4861 ehvtrdklnd finaktttsg taslhiaakn gsldivksll tygasynikn kegkapidvs
     4921 nnqdinslfr lieklfkdtk ngnvkvieti ealqpdefla vtnarnhlgs tllqvaifkk
     4981 rkyitskllk lmen
//
LOCUS       XP_076546271             294 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604238 [Osmia lignaria lignaria].
ACCESSION   XP_076546271
VERSION     XP_076546271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690156.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 50% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="uncharacterized protein LOC117604238"
                     /calculated_mol_wt=30002
     Region          <2..140
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..294
                     /gene="LOC117604238"
                     /coded_by="XM_076690156.1:1..885"
                     /db_xref="GeneID:117604238"
ORIGIN      
        1 mevvdmgeam avevedtgea tevavedtga dmvdmvevte vavedtgeat evavedtgea
       61 teeavedtgv dtvdtaeate vavedtgvat eeavedtgvd tvdtaeatev avedtgvate
      121 eavedtgvdt vdmaeateva hgggygghgg gggghggghg ggggghgggg hggggghggg
      181 ghahsfhhfs gpvvgghqev swkdkhghyd hdykaypkyh yaygvedsht kdyhgqkehr
      241 dgkevdgeyh lhepggnvrs vkyhshphgg ffaevhnygg ndhsggghgg hkhr
//
LOCUS       XP_076546272             328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305670 [Osmia lignaria lignaria].
ACCESSION   XP_076546272
VERSION     XP_076546272.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690157.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="uncharacterized protein LOC143305670"
                     /calculated_mol_wt=35418
     CDS             1..328
                     /gene="LOC143305670"
                     /coded_by="XM_076690157.1:1..987"
                     /db_xref="GeneID:143305670"
ORIGIN      
        1 maayttatfa slspssclsr srslflsrvc thiytyiyvy tvsigtftri hnatfvalvl
       61 nrfcpfpfsc kpcirlrctv ekhvpalrlf hrvsfvsvqi vflfsfghrk ssllpreprv
      121 hlrrapardg rvslvhrrla sppvpiptlv lrafrhpppp shppsrrrad srplrsspls
      181 tdypatrqfq plspqsgtgq vcwlarsfha yaqslsvhhr aripreslpr vrararfnps
      241 lfvasanfaa ssataaatsl sypfssssss sgsgsdsgsg sgsgsasasa sassrsssrp
      301 lhllappnav rpqhgapnai slslgfaa
//
LOCUS       XP_076546273             223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611838 [Osmia lignaria lignaria].
ACCESSION   XP_076546273
VERSION     XP_076546273.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690158.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="uncharacterized protein LOC117611838"
                     /calculated_mol_wt=26362
     CDS             1..223
                     /gene="LOC117611838"
                     /coded_by="XM_076690158.1:177..848"
                     /db_xref="GeneID:117611838"
ORIGIN      
        1 maefsvnvee vsdnqadrin clikeytelk kminkyktsr yywelhystl siniwsgqik
       61 ytklqenetr lrtsiiestk ltdfkeqelk nliakrgiyf seqcdnfrik stkftdnflr
      121 aqkeysvdil tkdikqycde yektnkelle lknkltklei dcgfnclsla eendeilkin
      181 avlidvrenn nikllkiksa qdmlkglkne ikqkkmltnf fnk
//
LOCUS       XP_076546274             455 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305671 [Osmia lignaria lignaria].
ACCESSION   XP_076546274
VERSION     XP_076546274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690159.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 38% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..455
                     /product="uncharacterized protein LOC143305671"
                     /calculated_mol_wt=50557
     CDS             1..455
                     /gene="LOC143305671"
                     /coded_by="XM_076690159.1:1..1368"
                     /db_xref="GeneID:143305671"
ORIGIN      
        1 makwifvlsv aavltrigma dimvqpgyqs pqhhsiitqp hetmqhlnkd hlqqpksady
       61 hlefhpvytd syqipanaly pvlqhyqvmp qlygthyshp nsgdvglpgm hsseyvplpv
      121 hsryrrsses ndgeqsekrd kldavatkss ldenvspvid lrslggeage gldeknvkvr
      181 qsdnnqqpit sntlfglnpa nmyptyqqyq fvdpalnsnq efrniemlpe gtvstepase
      241 yrevssaple spqtklenlq hamllqhpgp eqqqqsevsr sselrnfpdp rpssatlqsp
      301 eadtsdnhse sraatrgtkf vsvnthkklg vvkvgyphnt kvvknyhhha vvgnfrhapd
      361 gshpsvhpay tihqkndgml nnfatnsgqq evqvyqlpng qattclpasq ytilpqtygs
      421 nyvanyalgs psssstyqic vhnpdnlpfv myqlp
//
LOCUS       XP_034191335             458 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609291 [Osmia lignaria lignaria].
ACCESSION   XP_034191335
VERSION     XP_034191335.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335444.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191335.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..458
                     /product="uncharacterized protein LOC117609291"
                     /calculated_mol_wt=53909
     CDS             1..458
                     /gene="LOC117609291"
                     /coded_by="XM_034335444.2:148..1524"
                     /db_xref="GeneID:117609291"
ORIGIN      
        1 mirwspsvdr ksprfsltls kltlfppfvh kndesffett kekpkktrrd rrpptkketi
       61 slgdnistck shlaessakn dpaeyvlckd dcgwktmtck elhdtieeet kkftrvifdi
      121 phedqeeeee yefedetdpd eekdemgswe lgecesfssl sdneeepntt dpcmshelyl
      181 pyykiqrpry festntesgn iiirlprrpr spvrqilpdk felknaymew drkrrvserr
      241 kyleeskeri sysigrlsma pidertliss mvlqmvceqv tltlmdveid vdvqtfvnia
      301 avwalseldi fdyggllder ytqpenarff nwkqqdiidv mgtggmrkil mgayrppfnr
      361 nlftnfhrrl pwpsvspsha rgypkfypnv reridektkg seeapsklth vpnltcfhlr
      421 evldksyvay rrksksfflr ffsteeksdl fiqllslm
//
LOCUS       XP_076546275             141 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305672 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546275
VERSION     XP_076546275.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690160.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 50% of CDS bases
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..141
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305672"
                     /calculated_mol_wt=14573
     CDS             1..141
                     /gene="LOC143305672"
                     /coded_by="XM_076690160.1:1..426"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 2 bases in 1 codon;
                     substituted 1 base at 1 genomic stop codon"
                     /db_xref="GeneID:143305672"
ORIGIN      
        1 makrcfsvnv vgadgkfdfs ivvledlhat gsttlxarvp psgtrxidrs vslqrprrei
       61 fsssrnnswg pnyrvsccve gaqtgvssrl llrrlplhpt dackrnafsg ggegfvslda
      121 fssgwgpvgg gemisvlagg s
//
LOCUS       XP_076546276             240 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305673 [Osmia lignaria lignaria].
ACCESSION   XP_076546276
VERSION     XP_076546276.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690161.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="uncharacterized protein LOC143305673"
                     /calculated_mol_wt=26854
     Region          86..>149
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..240
                     /gene="LOC143305673"
                     /coded_by="XM_076690161.1:520..1242"
                     /db_xref="GeneID:143305673"
ORIGIN      
        1 mqyiigktta aefcdkfeet irnyenspga tllseeekrd afynavmisv pniqtvefvk
       61 nskgattsyn dlkllvmkde avreqmsrss tevrsanqal vdsrrcyecq ayghigamcp
      121 nrgqgimcyh ckrignhrap dcphkpsgsr gavrqggpsr gnsrynisyk fnnctrdsdg
      181 rlgqrgvlkr kreitkasev krgrfnihks kgrrvyknlh yrggsqpakd eskgegkgsq
//
LOCUS       XP_034194798             555 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611018 [Osmia lignaria lignaria].
ACCESSION   XP_034194798
VERSION     XP_034194798.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338907.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194798.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..555
                     /product="uncharacterized protein LOC117611018"
                     /calculated_mol_wt=64626
     Region          11..151
                     /region_name="Exo_endo_phos"
                     /note="Endonuclease/Exonuclease/phosphatase family;
                     pfam03372"
                     /db_xref="CDD:460902"
     Region          <113..222
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Region          505..>548
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..555
                     /gene="LOC117611018"
                     /coded_by="XM_034338907.2:1..1668"
                     /db_xref="GeneID:117611018"
ORIGIN      
        1 medrswtigf wncagiankd vefwetikew dvvilietwl eekgwdrird rmpkgyrwea
       61 qlakrknkkg ramggmimgv kkgilgkegg irveeekegi imaeckggkl nwrivgvyvn
      121 gdmeakikdl rpwleeigeg rrtiiggdfn artgeevgsk reeedeegee krrskdkksn
      181 aegnrlletl retgwtilng tiegdkdgef tytgargetv idyviveeeg reeiirmevg
      241 driesdhhpl ivemrgggeq yiieerrrkt tprgdwseqg rkdfiegisw rrsknkklna
      301 dmkitleefr kglergnkke rkskkqgwwd eeckerkkev rkilrewrrg skegesyrka
      361 kqeynkmcee kkkeenerfi kevegvktdt dvlrivnrer kkrgqiqsni keeewkshfm
      421 dllggteenv vmggertaee deeeeiskee irkavaklkd gkavggdgip nevwkyggee
      481 iaewvwktcn rvwkgegwpe ewkggiiapi kkkgngrqas dyrgvtlmpt lykiyaavlt
      541 erlrqevenk rlipe
//
LOCUS       XP_034176660            2242 aa            linear   INV 03-SEP-2025
DEFINITION  shroom isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034176660
VERSION     XP_034176660.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176660.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2242
                     /product="shroom isoform X1"
                     /calculated_mol_wt=249765
     Region          1935..2241
                     /region_name="ASD2"
                     /note="Apx/Shroom domain ASD2; pfam08687"
                     /db_xref="CDD:462561"
     CDS             1..2242
                     /gene="Shrm"
                     /coded_by="XM_034320769.2:1294..8022"
                     /db_xref="GeneID:117602571"
ORIGIN      
        1 mddnprnglq adqwrpmfek gekrantlgs gafrfirtrs sirrelkrqc nrskngdnpm
       61 vqeagngnsg gglgkwlreh lgggskgqgq dasspletnt gsivgnsgne kpcgrtvyts
      121 qnlycslpre hgrhhaynaa rktghensvk lqgydrqrca svnhhekttl sdlgtltrgg
      181 piygsegktp kkrnrdrrrh slnethehsr qrrrrcipdg gdvgytmtel qpsppgyrvq
      241 deapgppscp pgpykyashg hgsepanfks assypqegyg lkqspsrtva sneyyrrrnd
      301 grrsaenehe agnaakkatv agyneshkkn tagykndsgy seslgfdsyt lplnerdeas
      361 ppptppvrda sslkgvcygp ghekypswps aperhpdedv hgsghsgshr skswtdhtny
      421 pkekpaqytr phtkrpnptf tqqlktvmer cekipaetfe srnrsnveee prlwprvdre
      481 gkalgdaeyv vpsppereqq taqtlshadl eayvrsyqdp qvsqvdiyre gtltqaglee
      541 ytrvqhsqqa syaqsegyhs yvssvdsttn tpfldrlrrd seavaqrpts twedtsregr
      601 dsvvttssgs asssetlkwh gsmsdvsvss glparqgasd rwhhgsmsdv ssvnggilsq
      661 kanngchdkw qgsmsdvssn glspttkgrh nekwpdkwqv smndrnkqsq grpslpavin
      721 hcnastnvtd vsssvreskw qessideesl vdktqsnsqw dnsarietdk ygslaqpmpq
      781 spirqqigpt tpqspenwnp ihgsmsdvsq anglpcskql iahsarvqtp qrhhsesvly
      841 ldrernqrkl ypvsttqpqe stqssrmasa lpqqpqmsva erinelekqq qqqqqqqqmr
      901 ytyldpekrh rvsdptlkai qkkallsfye rhhqaswrse pqlaqgaapa pqspppqppp
      961 rprppssrra ssasdyasgt wrengnrnqn qsngelsspk hqhsnscgsl stdllgpviv
     1021 gpaisiddwv perppkkphl rnvyndrvps pdlpppsppt vtenevhdcd dplpppppel
     1081 sddcftenhr kpgnahhqpd eskvrerscd rhklerhsmr rskhgakreh ekssgksspn
     1141 sptktvsqeq eqhqfvrsai alkhvdsemv lengvsagfa thrmvatgrs slrypstqkl
     1201 mvngrvtpar riseernfpn rppaqlpdli sqrysdsgnq rpapqvpvep riirqesmrv
     1261 dgtridvsgl irndsaqrle sssgqrpqpq vpksidksat tnqsnpttrp nylpvtensk
     1321 catkyleggn hgftigspvk tgyeanskmf paesspqkyh eppkyvpnhh qrhgdatqrn
     1381 yyalppkyid apkqkpqpqc ptdryggsgs psspppplap rqntpgrksl pppprpapph
     1441 plqgsqskas ylayrrerga pdsegsykrt msptsrledw ppprdhddpv llrvtphhpl
     1501 qhhhhhhhnn hhsqqaelsk shsvdalhhr leersnqqqq qqqqqhtaev igklshdlnk
     1561 klqasearsr tsennnndnl edrhqhhhhh hhhqqhqekr hdyeqrrerl spqsvevlnd
     1621 rnrqlererr klaasceplp hnrekqsrsm dapssvveed ffrersatie mtsqnielln
     1681 rrnekrtnvt ststatittc ittsststtm tiatdncwsr gqealpfsdk ppppvssppr
     1741 spsdqveads prgavprrsg scssssssgr ssdprvsprn lsnslskies ssnnrkvcsp
     1801 tqtdntgssn eielrvdrss sstqksragg rsssssissi skasssssar nspveedkss
     1861 aaspcvspqp giegltlvqr tevvlrvnat tidaasqtdi lepveqpesi akgqeiplcr
     1921 kklpeeiece elgrdlasql spndklvpll vpapehkkpt dyvsglfrle atlqprprrr
     1981 lsleepttpc sengsdeekk petipsapvt plstdstspl sptsayftts egkarflary
     2041 srdstveslt rqedeppvip tdsldlrqkk eelmmrldkk lvvlraeqea vreegevnea
     2101 lgarvstris avarpaeask yrlhveevgk itslllglsg rlaraenaly gmpadhaerk
     2161 ileskrdklm dqleeakilk snidkrsvnv stilskylne eefadyqhfi nmkaklivdg
     2221 reiqdkvklg eeqlaalkea id
//
LOCUS       XP_034176678            2118 aa            linear   INV 03-SEP-2025
DEFINITION  shroom isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034176678
VERSION     XP_034176678.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320787.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176678.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2118
                     /product="shroom isoform X2"
                     /calculated_mol_wt=236474
     Region          1811..2117
                     /region_name="ASD2"
                     /note="Apx/Shroom domain ASD2; pfam08687"
                     /db_xref="CDD:462561"
     CDS             1..2118
                     /gene="Shrm"
                     /coded_by="XM_034320787.2:1293..7649"
                     /db_xref="GeneID:117602571"
ORIGIN      
        1 mddnprnglq adqwrpmfek gekrantlgs gafrfirtrs sirrelkrqc nrskngdnpm
       61 vqeagngnsg gglgkwlreh lgggskgqgq dasspletnt gsivgnsgne kpcgrtvyts
      121 qnlycslpre hgrhhaynaa rktghensvk lqgydrqrca svnhhekttl sdlgtltrgg
      181 piygsegktp kkrnrdrrrh slnethehsr qrrrrysesl gfdsytlpln erdeaspppt
      241 ppvrdasslk gvcygpghek ypswpsaper hpdedvhgsg hsgshrsksw tdhtnypkek
      301 paqytrphtk rpnptftqql ktvmerceki paetfesrnr snveeeprlw prvdregkal
      361 gdaeyvvpsp pereqqtaqt lshadleayv rsyqdpqvsq vdiyregtlt qagleeytrv
      421 qhsqqasyaq segyhsyvss vdsttntpfl drlrrdseav aqrptstwed tsregrdsvv
      481 ttssgsasss etlkwhgsms dvsvssglpa rqgasdrwhh gsmsdvssvn ggilsqkann
      541 gchdkwqgsm sdvssnglsp ttkgrhnekw pdkwqvsmnd rnkqsqgrps lpavinhcna
      601 stnvtdvsss vreskwqess ideeslvdkt qsnsqwdnsa rietdkygsl aqpmpqspir
      661 qqigpttpqs penwnpihgs msdvsqangl pcskqliahs arvqtpqrhh sesvlyldre
      721 rnqrklypvs ttqpqestqs srmasalpqq pqmsvaerin elekqqqqqq qqqqmrytyl
      781 dpekrhrvsd ptlkaiqkka llsfyerhhq aswrsepqla qgaapapqsp ppqppprprp
      841 pssrrassas dyasgtwren gnrnqnqsng elsspkhqhs nscgslstdl lgpvivgpai
      901 siddwvperp pkkphlrnvy ndrvpspdlp ppspptvten evhdcddplp ppppelsddc
      961 ftenhrkpgn ahhqpdeskv rerscdrhkl erhsmrrskh gakrehekss gksspnsptk
     1021 tvsqeqeqhq fvrsaialkh vdsemvleng vsagfathrm vatgrsslry pstqklmvng
     1081 rvtparrise ernfpnrppa qlpdlisqry sdsgnqrpap qvpvepriir qesmrvdgtr
     1141 idvsglirnd saqrlesssg qrpqpqvpks idksattnqs npttrpnylp vtenskcatk
     1201 yleggnhgft igspvktgye anskmfpaes spqkyheppk yvpnhhqrhg datqrnyyal
     1261 ppkyidapkq kpqpqcptdr yggsgspssp ppplaprqnt pgrkslpppp rpapphplqg
     1321 sqskasylay rrergapdse gsykrtmspt srledwpppr dhddpvllrv tphhplqhhh
     1381 hhhhnnhhsq qaelskshsv dalhhrleer snqqqqqqqq qhtaevigkl shdlnkklqa
     1441 searsrtsen nnndnledrh qhhhhhhhhq qhqekrhdye qrrerlspqs vevlndrnrq
     1501 lererrklaa sceplphnre kqsrsmdaps svveedffre rsatiemtsq niellnrrne
     1561 krtnvtstst atittcitts ststtmtiat dncwsrgqea lpfsdkpppp vsspprspsd
     1621 qveadsprga vprrsgscss ssssgrssdp rvsprnlsns lskiesssnn rkvcsptqtd
     1681 ntgssneiel rvdrsssstq ksraggrsss ssissiskas ssssarnspv eedkssaasp
     1741 cvspqpgieg ltlvqrtevv lrvnattida asqtdilepv eqpesiakgq eiplcrkklp
     1801 eeieceelgr dlasqlspnd klvpllvpap ehkkptdyvs glfrleatlq prprrrlsle
     1861 epttpcseng sdeekkpeti psapvtplst dstsplspts ayfttsegka rflarysrds
     1921 tvesltrqed eppviptdsl dlrqkkeelm mrldkklvvl raeqeavree gevnealgar
     1981 vstrisavar paeaskyrlh veevgkitsl llglsgrlar aenalygmpa dhaerkiles
     2041 krdklmdqle eakilksnid krsvnvstil skylneeefa dyqhfinmka klivdgreiq
     2101 dkvklgeeql aalkeaid
//
LOCUS       XP_034176687            2104 aa            linear   INV 03-SEP-2025
DEFINITION  shroom isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034176687
VERSION     XP_034176687.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320796.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176687.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2104
                     /product="shroom isoform X3"
                     /calculated_mol_wt=234959
     Region          1797..2103
                     /region_name="ASD2"
                     /note="Apx/Shroom domain ASD2; pfam08687"
                     /db_xref="CDD:462561"
     CDS             1..2104
                     /gene="Shrm"
                     /coded_by="XM_034320796.2:350..6664"
                     /db_xref="GeneID:117602571"
ORIGIN      
        1 mmpsfhtetv sdnetgvrhf klyilmivar ivrgilrwri yvpartclec frdtrlryar
       61 pelrnrpgnp ysrnplwcip dggdvgytmt elqpsppgyr vqdeapgpps cppgpykyas
      121 hghgsepanf ksassypqeg yglkqspsrt vasneyyrrr ndgrrsaene heagnaakka
      181 tvagyneshk kntagyknds gyseslgfds ytlplnerde asppptppvr dasslkgvcy
      241 gpghekypsw psaperhpde dvhgsghsgs hrskswtdht nypkekpaqy trphtkrpnp
      301 tftqqlktvm ercekipaet fesrnrsnve eeprlwprvd regkalgdae yvvpsppere
      361 qqtaqtlsha dleayvrsyq dpqvsqvdiy regtltqagl eeytrvqhsq qasyaqsegy
      421 hsyvssvdst tntpfldrlr rdseavaqrp tstwedtsre grdsvvttss gsasssetlk
      481 whgsmsdvsv ssglparqga sdrwhhgsms dvssvnggil sqkanngchd kwqgsmsdvs
      541 snglspttkg rhnekwpdkw qvsmndrnkq sqgrpslpav inhcnastnv tdvsssvres
      601 kwqessidee slvdktqsns qwdnsariet dkygslaqpm pqspirqqig pttpqspenw
      661 npihgsmsdv sqanglpcsk qliahsarvq tpqrhhsesv lyldrernqr klypvsttqp
      721 qestqssrma salpqqpqms vaerinelek qqqqqqqqqq mrytyldpek rhrvsdptlk
      781 aiqkkallsf yerhhqaswr sepqlaqgaa papqspppqp pprprppssr rassasdyas
      841 gtwrengnrn qnqsngelss pkhqhsnscg slstdllgpv ivgpaisidd wvperppkkp
      901 hlrnvyndrv pspdlpppsp ptvtenevhd cddplppppp elsddcften hrkpgnahhq
      961 pdeskvrers cdrhklerhs mrrskhgakr ehekssgkss pnsptktvsq eqeqhqfvrs
     1021 aialkhvdse mvlengvsag fathrmvatg rsslrypstq klmvngrvtp arriseernf
     1081 pnrppaqlpd lisqrysdsg nqrpapqvpv epriirqesm rvdgtridvs glirndsaqr
     1141 lesssgqrpq pqvpksidks attnqsnptt rpnylpvten skcatkyleg gnhgftigsp
     1201 vktgyeansk mfpaesspqk yheppkyvpn hhqrhgdatq rnyyalppky idapkqkpqp
     1261 qcptdryggs gspssppppl aprqntpgrk slpppprpap phplqgsqsk asylayrrer
     1321 gapdsegsyk rtmsptsrle dwppprdhdd pvllrvtphh plqhhhhhhh nnhhsqqael
     1381 skshsvdalh hrleersnqq qqqqqqqhta evigklshdl nkklqasear srtsennnnd
     1441 nledrhqhhh hhhhhqqhqe krhdyeqrre rlspqsvevl ndrnrqlere rrklaascep
     1501 lphnrekqsr smdapssvve edffrersat iemtsqniel lnrrnekrtn vtststatit
     1561 tcittsstst tmtiatdncw srgqealpfs dkppppvssp prspsdqvea dsprgavprr
     1621 sgscssssss grssdprvsp rnlsnslski esssnnrkvc sptqtdntgs sneielrvdr
     1681 sssstqksra ggrsssssis siskasssss arnspveedk ssaaspcvsp qpgiegltlv
     1741 qrtevvlrvn attidaasqt dilepveqpe siakgqeipl crkklpeeie ceelgrdlas
     1801 qlspndklvp llvpapehkk ptdyvsglfr leatlqprpr rrlsleeptt pcsengsdee
     1861 kkpetipsap vtplstdsts plsptsayft tsegkarfla rysrdstves ltrqedeppv
     1921 iptdsldlrq kkeelmmrld kklvvlraeq eavreegevn ealgarvstr isavarpaea
     1981 skyrlhveev gkitslllgl sgrlaraena lygmpadhae rkileskrdk lmdqleeaki
     2041 lksnidkrsv nvstilskyl neeefadyqh finmkakliv dgreiqdkvk lgeeqlaalk
     2101 eaid
//
LOCUS       XP_034176694            2016 aa            linear   INV 03-SEP-2025
DEFINITION  shroom isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034176694
VERSION     XP_034176694.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320803.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176694.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2016
                     /product="shroom isoform X4"
                     /calculated_mol_wt=224591
     Region          1709..2015
                     /region_name="ASD2"
                     /note="Apx/Shroom domain ASD2; pfam08687"
                     /db_xref="CDD:462561"
     CDS             1..2016
                     /gene="Shrm"
                     /coded_by="XM_034320803.2:199..6249"
                     /db_xref="GeneID:117602571"
ORIGIN      
        1 mtelqpsppg yrvqdeapgp pscppgpyky ashghgsepa nfksassypq egyglkqsps
       61 rtvasneyyr rrndgrrsae neheagnaak katvagynes hkkntagykn dsgyseslgf
      121 dsytlplner deasppptpp vrdasslkgv cygpghekyp swpsaperhp dedvhgsghs
      181 gshrskswtd htnypkekpa qytrphtkrp nptftqqlkt vmercekipa etfesrnrsn
      241 veeeprlwpr vdregkalgd aeyvvpsppe reqqtaqtls hadleayvrs yqdpqvsqvd
      301 iyregtltqa gleeytrvqh sqqasyaqse gyhsyvssvd sttntpfldr lrrdseavaq
      361 rptstwedts regrdsvvtt ssgsassset lkwhgsmsdv svssglparq gasdrwhhgs
      421 msdvssvngg ilsqkanngc hdkwqgsmsd vssnglsptt kgrhnekwpd kwqvsmndrn
      481 kqsqgrpslp avinhcnast nvtdvsssvr eskwqessid eeslvdktqs nsqwdnsari
      541 etdkygslaq pmpqspirqq igpttpqspe nwnpihgsms dvsqanglpc skqliahsar
      601 vqtpqrhhse svlyldrern qrklypvstt qpqestqssr masalpqqpq msvaerinel
      661 ekqqqqqqqq qqmrytyldp ekrhrvsdpt lkaiqkkall sfyerhhqas wrsepqlaqg
      721 aapapqsppp qppprprpps srrassasdy asgtwrengn rnqnqsngel sspkhqhsns
      781 cgslstdllg pvivgpaisi ddwvperppk kphlrnvynd rvpspdlppp spptvtenev
      841 hdcddplppp ppelsddcft enhrkpgnah hqpdeskvre rscdrhkler hsmrrskhga
      901 krehekssgk sspnsptktv sqeqeqhqfv rsaialkhvd semvlengvs agfathrmva
      961 tgrsslryps tqklmvngrv tparriseer nfpnrppaql pdlisqrysd sgnqrpapqv
     1021 pvepriirqe smrvdgtrid vsglirndsa qrlesssgqr pqpqvpksid ksattnqsnp
     1081 ttrpnylpvt enskcatkyl eggnhgftig spvktgyean skmfpaessp qkyheppkyv
     1141 pnhhqrhgda tqrnyyalpp kyidapkqkp qpqcptdryg gsgspssppp plaprqntpg
     1201 rkslpppprp apphplqgsq skasylayrr ergapdsegs ykrtmsptsr ledwppprdh
     1261 ddpvllrvtp hhplqhhhhh hhnnhhsqqa elskshsvda lhhrleersn qqqqqqqqqh
     1321 taevigklsh dlnkklqase arsrtsennn ndnledrhqh hhhhhhhqqh qekrhdyeqr
     1381 rerlspqsve vlndrnrqle rerrklaasc eplphnrekq srsmdapssv veedffrers
     1441 atiemtsqni ellnrrnekr tnvtststat ittcittsst sttmtiatdn cwsrgqealp
     1501 fsdkppppvs spprspsdqv eadsprgavp rrsgscssss ssgrssdprv sprnlsnsls
     1561 kiesssnnrk vcsptqtdnt gssneielrv drsssstqks raggrsssss issiskasss
     1621 ssarnspvee dkssaaspcv spqpgieglt lvqrtevvlr vnattidaas qtdilepveq
     1681 pesiakgqei plcrkklpee ieceelgrdl asqlspndkl vpllvpapeh kkptdyvsgl
     1741 frleatlqpr prrrlsleep ttpcsengsd eekkpetips apvtplstds tsplsptsay
     1801 fttsegkarf larysrdstv esltrqedep pviptdsldl rqkkeelmmr ldkklvvlra
     1861 eqeavreege vnealgarvs trisavarpa easkyrlhve evgkitslll glsgrlarae
     1921 nalygmpadh aerkileskr dklmdqleea kilksnidkr svnvstilsk ylneeefady
     1981 qhfinmkakl ivdgreiqdk vklgeeqlaa lkeaid
//
LOCUS       XP_034176696            2016 aa            linear   INV 03-SEP-2025
DEFINITION  shroom isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034176696
VERSION     XP_034176696.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320805.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034176696.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2016
                     /product="shroom isoform X4"
                     /calculated_mol_wt=224591
     Region          1709..2015
                     /region_name="ASD2"
                     /note="Apx/Shroom domain ASD2; pfam08687"
                     /db_xref="CDD:462561"
     CDS             1..2016
                     /gene="Shrm"
                     /coded_by="XM_034320805.2:162..6212"
                     /db_xref="GeneID:117602571"
ORIGIN      
        1 mtelqpsppg yrvqdeapgp pscppgpyky ashghgsepa nfksassypq egyglkqsps
       61 rtvasneyyr rrndgrrsae neheagnaak katvagynes hkkntagykn dsgyseslgf
      121 dsytlplner deasppptpp vrdasslkgv cygpghekyp swpsaperhp dedvhgsghs
      181 gshrskswtd htnypkekpa qytrphtkrp nptftqqlkt vmercekipa etfesrnrsn
      241 veeeprlwpr vdregkalgd aeyvvpsppe reqqtaqtls hadleayvrs yqdpqvsqvd
      301 iyregtltqa gleeytrvqh sqqasyaqse gyhsyvssvd sttntpfldr lrrdseavaq
      361 rptstwedts regrdsvvtt ssgsassset lkwhgsmsdv svssglparq gasdrwhhgs
      421 msdvssvngg ilsqkanngc hdkwqgsmsd vssnglsptt kgrhnekwpd kwqvsmndrn
      481 kqsqgrpslp avinhcnast nvtdvsssvr eskwqessid eeslvdktqs nsqwdnsari
      541 etdkygslaq pmpqspirqq igpttpqspe nwnpihgsms dvsqanglpc skqliahsar
      601 vqtpqrhhse svlyldrern qrklypvstt qpqestqssr masalpqqpq msvaerinel
      661 ekqqqqqqqq qqmrytyldp ekrhrvsdpt lkaiqkkall sfyerhhqas wrsepqlaqg
      721 aapapqsppp qppprprpps srrassasdy asgtwrengn rnqnqsngel sspkhqhsns
      781 cgslstdllg pvivgpaisi ddwvperppk kphlrnvynd rvpspdlppp spptvtenev
      841 hdcddplppp ppelsddcft enhrkpgnah hqpdeskvre rscdrhkler hsmrrskhga
      901 krehekssgk sspnsptktv sqeqeqhqfv rsaialkhvd semvlengvs agfathrmva
      961 tgrsslryps tqklmvngrv tparriseer nfpnrppaql pdlisqrysd sgnqrpapqv
     1021 pvepriirqe smrvdgtrid vsglirndsa qrlesssgqr pqpqvpksid ksattnqsnp
     1081 ttrpnylpvt enskcatkyl eggnhgftig spvktgyean skmfpaessp qkyheppkyv
     1141 pnhhqrhgda tqrnyyalpp kyidapkqkp qpqcptdryg gsgspssppp plaprqntpg
     1201 rkslpppprp apphplqgsq skasylayrr ergapdsegs ykrtmsptsr ledwppprdh
     1261 ddpvllrvtp hhplqhhhhh hhnnhhsqqa elskshsvda lhhrleersn qqqqqqqqqh
     1321 taevigklsh dlnkklqase arsrtsennn ndnledrhqh hhhhhhhqqh qekrhdyeqr
     1381 rerlspqsve vlndrnrqle rerrklaasc eplphnrekq srsmdapssv veedffrers
     1441 atiemtsqni ellnrrnekr tnvtststat ittcittsst sttmtiatdn cwsrgqealp
     1501 fsdkppppvs spprspsdqv eadsprgavp rrsgscssss ssgrssdprv sprnlsnsls
     1561 kiesssnnrk vcsptqtdnt gssneielrv drsssstqks raggrsssss issiskasss
     1621 ssarnspvee dkssaaspcv spqpgieglt lvqrtevvlr vnattidaas qtdilepveq
     1681 pesiakgqei plcrkklpee ieceelgrdl asqlspndkl vpllvpapeh kkptdyvsgl
     1741 frleatlqpr prrrlsleep ttpcsengsd eekkpetips apvtplstds tsplsptsay
     1801 fttsegkarf larysrdstv esltrqedep pviptdsldl rqkkeelmmr ldkklvvlra
     1861 eqeavreege vnealgarvs trisavarpa easkyrlhve evgkitslll glsgrlarae
     1921 nalygmpadh aerkileskr dklmdqleea kilksnidkr svnvstilsk ylneeefady
     1981 qhfinmkakl ivdgreiqdk vklgeeqlaa lkeaid
//
LOCUS       XP_034176742             631 aa            linear   INV 03-SEP-2025
DEFINITION  heat shock 70 kDa protein cognate 4-like [Osmia lignaria lignaria].
ACCESSION   XP_034176742
VERSION     XP_034176742.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320851.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="heat shock 70 kDa protein cognate 4-like"
                     /calculated_mol_wt=69643
     Region          1..631
                     /region_name="PTZ00009"
                     /note="heat shock 70 kDa protein; Provisional"
                     /db_xref="CDD:240227"
     CDS             1..631
                     /gene="LOC117602627"
                     /coded_by="XM_034320851.2:106..2001"
                     /db_xref="GeneID:117602627"
ORIGIN      
        1 maapavgidl gttyscvgvf ksgkveiian dqgnrttpsy vaftdterli gdaaknqvam
       61 nptntifdak rligrrfdds svqadlkhwp favindggkp kikvhykset ktffpeeiss
      121 mvltkmketa eaylgkaitn avitvpayfn dsqrqatkda gtisglnvlr iineptaaai
      181 aygldkkgrs ernvlifdlg ggtfdvsilt indgifevka tagdthlgge dfdnrmvthf
      241 aqefsrkfkk dltknkrair rlqtacerak rtlssstqan ieidslldgi dfytsitrar
      301 feelnqdlfr gtlqpveesl rdakmdksqi hdivlvggst ripriqrllq dffngkdlnk
      361 sinadeavay gaavqaailq gdrsetvqdl llldvtplsl gietaggvmt alikrnstip
      421 tkqtqtftty sdnqpsvliq vyegeramtk dnnllgkfel sgippaprgm pqievtfdid
      481 angilnvsav ekstnkenki titndkgrls kedidrmire aeryqtedqs qkdkvaaknn
      541 lesycfnmkn avedekikdk iaegdrkria dkcneiikwl dnnvsaskee fegklkdvek
      601 ickpimtkfy eggasnygqa gadgptieev d
//
LOCUS       XP_034176754             434 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602633 [Osmia lignaria lignaria].
ACCESSION   XP_034176754
VERSION     XP_034176754.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320863.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..434
                     /product="uncharacterized protein LOC117602633"
                     /calculated_mol_wt=50967
     Region          249..416
                     /region_name="DUF4201"
                     /note="Domain of unknown function (DUF4201); pfam13870"
                     /db_xref="CDD:464008"
     CDS             1..434
                     /gene="LOC117602633"
                     /coded_by="XM_034320863.2:299..1603"
                     /db_xref="GeneID:117602633"
ORIGIN      
        1 mstgrsfpfn avdeqepidf edfskyelds waegneeeee geergergks tdedstewnv
       61 vakwsdeges yhegeevgea eelfsstiss sekleeerpv iedsvipaev sadreaiinr
      121 lkelqdelse lrrknkmlel wifrqmkktq eritpsdlnk tieqmeevyk ealraykmqi
      181 deissqqael ksefedylkr mqtireeeqq afdaflnrek evaidlvfek tgkkitekmm
      241 nglmrrqtar rivlardrmr yvflqhrled lkvrlrvaet lgegmttmdy ealhvanmgy
      301 knrlderdhe leklrikiae tvngvaqyke keicivedie ygkqnlnmcr eastnirerv
      361 nrarltlndv rstlnekkle agllvakqel lemekmmklk gllkekinel qrevqryeva
      421 khklqeeiga srqh
//
LOCUS       XP_034196215            1165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611893 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196215
VERSION     XP_034196215.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340324.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196215.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1165
                     /product="uncharacterized protein LOC117611893 isoform X1"
                     /calculated_mol_wt=133353
     Region          35..226
                     /region_name="DEADc_DDX20"
                     /note="DEAD-box helicase domain of DEAD box protein 20;
                     cd17943"
                     /db_xref="CDD:350701"
     Site            order(44,46,48,51,69..76)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350701"
     Region          237..374
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(333,335,360,363..364)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..1165
                     /gene="LOC117611893"
                     /coded_by="XM_034340324.2:188..3685"
                     /db_xref="GeneID:117611893"
ORIGIN      
        1 mariiahdis kmprtkdiki qedvtffqmg fsqkildgla ncgflkpspi qlkaiplgrc
       61 gfdlimraks gtgktlvfsi ialemldvev ssvqalilap treiavqise vfssvgseik
      121 glkvqvfigg tpidndkkkl nnchvavgap grirhlidkg llnvenvrlf vldeadklme
      181 tsfqkdinyi fsklplnkqv iassatyfgd letflqtymc spivtspdnd epilaglkqf
      241 vavvpfhpna mrqvqikvde lvkifntvpf kqslvfsnyq sraqsvcnki nsmgfsatyi
      301 agnqdmtkrl dainklktfk crimittdlt argidadnvn lvvnldvpid gptylhrigr
      361 agrygsygis itivgdneve tfrellnsvg gpnfdvlklp vsypndiwst sdtlfeklha
      421 rsdvnenqfe ikfevnstnd lppgavyrel qnnihvedsa stvdinkent dntdsidknv
      481 dnfqknafnl kassksgrik vsslfaqnvn klneseeikv nkedtitiks yssskpkvih
      541 nfklhfesnn lstwekankd tlfkvdltdv qednlcssdi diitehleyn rydkslkess
      601 srendtklps hfenmisknd tekpiasawe dnnvtisnei qystdvyclk elkhyvseqm
      661 erfsksenel psndeetlin ealgwkqile feiklldttm elmkesvqkl vyqehidmlr
      721 ifykiqkhai lcvypeirsd deiddtylyf gnpsgenllt mfkeiedfks syripgqkln
      781 ayfpypvekd sympnlmilk tdldnyrnsl kylnsdpypr ekllqivnyv afiseikkhn
      841 likklkaqsl sfnellatik velgkesfek egqftddntr divqdelhaq shtntdeqne
      901 iteeydsnns ltnsvqndys kiqelachrl qnntcttsse sddfqehkmn rkvyskskfh
      961 rwkknetqvn pvketekssy kiptnnnldd vdrlcnntds yekksnaeln syagnrqssk
     1021 hkhksescth dtlnsyktvs arstrkvksh khlddehlkd ltnvyntdya qqskqgepln
     1081 pqyhiplyny ihnipsssqi enytyfpsnf ypenktnfyy pldknvnkcd tnidqflssl
     1141 raetdllhle lytsemlsnw tkdyn
//
LOCUS       XP_034196223            1165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611893 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196223
VERSION     XP_034196223.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340332.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196223.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1165
                     /product="uncharacterized protein LOC117611893 isoform X1"
                     /calculated_mol_wt=133353
     Region          35..226
                     /region_name="DEADc_DDX20"
                     /note="DEAD-box helicase domain of DEAD box protein 20;
                     cd17943"
                     /db_xref="CDD:350701"
     Site            order(44,46,48,51,69..76)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350701"
     Region          237..374
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(333,335,360,363..364)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..1165
                     /gene="LOC117611893"
                     /coded_by="XM_034340332.2:152..3649"
                     /db_xref="GeneID:117611893"
ORIGIN      
        1 mariiahdis kmprtkdiki qedvtffqmg fsqkildgla ncgflkpspi qlkaiplgrc
       61 gfdlimraks gtgktlvfsi ialemldvev ssvqalilap treiavqise vfssvgseik
      121 glkvqvfigg tpidndkkkl nnchvavgap grirhlidkg llnvenvrlf vldeadklme
      181 tsfqkdinyi fsklplnkqv iassatyfgd letflqtymc spivtspdnd epilaglkqf
      241 vavvpfhpna mrqvqikvde lvkifntvpf kqslvfsnyq sraqsvcnki nsmgfsatyi
      301 agnqdmtkrl dainklktfk crimittdlt argidadnvn lvvnldvpid gptylhrigr
      361 agrygsygis itivgdneve tfrellnsvg gpnfdvlklp vsypndiwst sdtlfeklha
      421 rsdvnenqfe ikfevnstnd lppgavyrel qnnihvedsa stvdinkent dntdsidknv
      481 dnfqknafnl kassksgrik vsslfaqnvn klneseeikv nkedtitiks yssskpkvih
      541 nfklhfesnn lstwekankd tlfkvdltdv qednlcssdi diitehleyn rydkslkess
      601 srendtklps hfenmisknd tekpiasawe dnnvtisnei qystdvyclk elkhyvseqm
      661 erfsksenel psndeetlin ealgwkqile feiklldttm elmkesvqkl vyqehidmlr
      721 ifykiqkhai lcvypeirsd deiddtylyf gnpsgenllt mfkeiedfks syripgqkln
      781 ayfpypvekd sympnlmilk tdldnyrnsl kylnsdpypr ekllqivnyv afiseikkhn
      841 likklkaqsl sfnellatik velgkesfek egqftddntr divqdelhaq shtntdeqne
      901 iteeydsnns ltnsvqndys kiqelachrl qnntcttsse sddfqehkmn rkvyskskfh
      961 rwkknetqvn pvketekssy kiptnnnldd vdrlcnntds yekksnaeln syagnrqssk
     1021 hkhksescth dtlnsyktvs arstrkvksh khlddehlkd ltnvyntdya qqskqgepln
     1081 pqyhiplyny ihnipsssqi enytyfpsnf ypenktnfyy pldknvnkcd tnidqflssl
     1141 raetdllhle lytsemlsnw tkdyn
//
LOCUS       XP_034196242            1165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611893 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196242
VERSION     XP_034196242.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340351.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196242.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1165
                     /product="uncharacterized protein LOC117611893 isoform X1"
                     /calculated_mol_wt=133353
     Region          35..226
                     /region_name="DEADc_DDX20"
                     /note="DEAD-box helicase domain of DEAD box protein 20;
                     cd17943"
                     /db_xref="CDD:350701"
     Site            order(44,46,48,51,69..76)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350701"
     Region          237..374
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(333,335,360,363..364)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     CDS             1..1165
                     /gene="LOC117611893"
                     /coded_by="XM_034340351.2:48..3545"
                     /db_xref="GeneID:117611893"
ORIGIN      
        1 mariiahdis kmprtkdiki qedvtffqmg fsqkildgla ncgflkpspi qlkaiplgrc
       61 gfdlimraks gtgktlvfsi ialemldvev ssvqalilap treiavqise vfssvgseik
      121 glkvqvfigg tpidndkkkl nnchvavgap grirhlidkg llnvenvrlf vldeadklme
      181 tsfqkdinyi fsklplnkqv iassatyfgd letflqtymc spivtspdnd epilaglkqf
      241 vavvpfhpna mrqvqikvde lvkifntvpf kqslvfsnyq sraqsvcnki nsmgfsatyi
      301 agnqdmtkrl dainklktfk crimittdlt argidadnvn lvvnldvpid gptylhrigr
      361 agrygsygis itivgdneve tfrellnsvg gpnfdvlklp vsypndiwst sdtlfeklha
      421 rsdvnenqfe ikfevnstnd lppgavyrel qnnihvedsa stvdinkent dntdsidknv
      481 dnfqknafnl kassksgrik vsslfaqnvn klneseeikv nkedtitiks yssskpkvih
      541 nfklhfesnn lstwekankd tlfkvdltdv qednlcssdi diitehleyn rydkslkess
      601 srendtklps hfenmisknd tekpiasawe dnnvtisnei qystdvyclk elkhyvseqm
      661 erfsksenel psndeetlin ealgwkqile feiklldttm elmkesvqkl vyqehidmlr
      721 ifykiqkhai lcvypeirsd deiddtylyf gnpsgenllt mfkeiedfks syripgqkln
      781 ayfpypvekd sympnlmilk tdldnyrnsl kylnsdpypr ekllqivnyv afiseikkhn
      841 likklkaqsl sfnellatik velgkesfek egqftddntr divqdelhaq shtntdeqne
      901 iteeydsnns ltnsvqndys kiqelachrl qnntcttsse sddfqehkmn rkvyskskfh
      961 rwkknetqvn pvketekssy kiptnnnldd vdrlcnntds yekksnaeln syagnrqssk
     1021 hkhksescth dtlnsyktvs arstrkvksh khlddehlkd ltnvyntdya qqskqgepln
     1081 pqyhiplyny ihnipsssqi enytyfpsnf ypenktnfyy pldknvnkcd tnidqflssl
     1141 raetdllhle lytsemlsnw tkdyn
//
LOCUS       XP_034196260            1118 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611893 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196260
VERSION     XP_034196260.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340369.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196260.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1118
                     /product="uncharacterized protein LOC117611893 isoform X2"
                     /calculated_mol_wt=127681
     Region          35..226
                     /region_name="DEADc_DDX20"
                     /note="DEAD-box helicase domain of DEAD box protein 20;
                     cd17943"
                     /db_xref="CDD:350701"
     Site            order(44,46,48,51,69..76)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350701"
     Region          237..374
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(333,335,360,363..364)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          <763..>1086
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     CDS             1..1118
                     /gene="LOC117611893"
                     /coded_by="XM_034340369.2:188..3544"
                     /db_xref="GeneID:117611893"
ORIGIN      
        1 mariiahdis kmprtkdiki qedvtffqmg fsqkildgla ncgflkpspi qlkaiplgrc
       61 gfdlimraks gtgktlvfsi ialemldvev ssvqalilap treiavqise vfssvgseik
      121 glkvqvfigg tpidndkkkl nnchvavgap grirhlidkg llnvenvrlf vldeadklme
      181 tsfqkdinyi fsklplnkqv iassatyfgd letflqtymc spivtspdnd epilaglkqf
      241 vavvpfhpna mrqvqikvde lvkifntvpf kqslvfsnyq sraqsvcnki nsmgfsatyi
      301 agnqdmtkrl dainklktfk crimittdlt argidadnvn lvvnldvpid gptylhrigr
      361 agrygsygis itivgdneve tfrellnsvg gpnfdvlklp vsypndiwst sdtlfeklha
      421 rsdvnenqfe ikfevnstnd lppgavyrel qnnihvedsa stvdinkent dntdsidknv
      481 dnfqknafnl kassksgrik vsslfaqnvn klneseeikv nkedtitiks yssskpkvih
      541 nfklhfesnn lstwekankd tlfkvdltdv qednlcssdi diitehleyn rydkslkess
      601 srendtklps hfenmisknd tekpiasawe dnnvtisnei qystdvyclk elkhyvseqm
      661 erfsksenel psndeetlin ealgwkqile feiklldttm elmkesvqkl vyqehidmlr
      721 ifykiqkhai lcvypeirsd deiddtylyf gnpsgenllt mfkeiedfks syripgqkln
      781 ayfpypvekd sympnlmilk tdldnyrnsl kylnsdpypr ekllqivnyv afiseikkhn
      841 likklkaqsl sfnellatik velgkesfek egqftddntr divqdelhaq shtntdeqne
      901 iteeydsnns ltnsvqndys kiqelachrl qnntcttsse sddfqehkmn rkvyskskfh
      961 rwkknetqvn pvketekssy kiptnnnldd vdrlcnntds yekksnaeln syagnrqssk
     1021 hkhksescth dtlnsyktvs arstrkvksh khlddehlkd ltnvyntdya qqskqdknvn
     1081 kcdtnidqfl sslraetdll hlelytseml snwtkdyn
//
LOCUS       XP_034196274             565 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611915 [Osmia lignaria lignaria].
ACCESSION   XP_034196274
VERSION     XP_034196274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340383.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..565
                     /product="uncharacterized protein LOC117611915"
                     /calculated_mol_wt=63927
     Region          12..92
                     /region_name="Wbp11"
                     /note="WW domain binding protein 11; pfam09429"
                     /db_xref="CDD:462795"
     CDS             1..565
                     /gene="LOC117611915"
                     /coded_by="XM_034340383.2:151..1848"
                     /db_xref="GeneID:117611915"
ORIGIN      
        1 mgrrsinttk sgkymnptdq arkearkkel kknkkqrqlv raavlkgkdp aqiieemeki
       61 dqmeynvmqp pplnekvlkd krkklketld rvlkmyakdd qekwaelkeq liqyerrrid
      121 lvsyfeavrh aqqvqvdeip lpsagndisr mysgsltsqi plpdmpqppt hiypphphyi
      181 pqhhplmnip ippsilkkts ayaaspsmpt lapnkeppgv ppfpppdlss ededvpekak
      241 dpapksrtir faddkedskq esdskdketd kdkekerdms kvkpttlqqk mlamagqdid
      301 qfmremevvh kkreteraqd lnarlsllee nesnsksnnk stnnkaeedd leppgasdhs
      361 snhnqhtssh shsqhtqpht mppmgmpppp lmyrpppppl hlrmpppppp riglrlppgp
      421 ppgmprmlrp gppsmprmpp pqmqmpnlsn msnignpqmq aqsatgsqpk tpnvlsaapq
      481 linrkdkdgk stttieakpq irnlaadvtr flptslrvkr ddkkkpsils rlseripetq
      541 pirtsqpktk ddaymqfmqe megll
//
LOCUS       XP_034183588             381 aa            linear   INV 03-SEP-2025
DEFINITION  misexpression suppressor of ras 6 [Osmia lignaria lignaria].
ACCESSION   XP_034183588
VERSION     XP_034183588.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327697.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..381
                     /product="misexpression suppressor of ras 6"
                     /calculated_mol_wt=43936
     Region          13..193
                     /region_name="UPF0489"
                     /note="UPF0489 domain; pfam12640"
                     /db_xref="CDD:432689"
     CDS             1..381
                     /gene="MESR6"
                     /coded_by="XM_034327697.2:317..1462"
                     /db_xref="GeneID:117605891"
ORIGIN      
        1 msfsrkyfkr vpiyvveahd evlpfiyrcl gskhlpfean vfvhldshpd mlipktmlad
       61 tvwdknelfs eisienwilp aayaghfkhl iwvkppwanq madgvttffi gkhkdngsir
      121 ltcpepyfvs eglytmmeel entrevtlhv mtiggfiddp akrddftais salrqylper
      181 dtpyildvdl dffstknpfk tlydranlyd klaslyafar pdstdpevlk eatdarneql
      241 neleslfnyl dehrslqgye gekssryeav eliyreltsv ykqseidwki ihdagctrdd
      301 tdlpdhvtkp qdldrlitgt frsflaalpa pptivtiars sedeycpied vdqiqlgvld
      361 elrqrlenvd iqlayqeeet h
//
LOCUS       XP_076546278            1235 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603362 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546278
VERSION     XP_076546278.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690163.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1235
                     /product="uncharacterized protein LOC117603362 isoform X1"
                     /calculated_mol_wt=134569
     Region          228..250
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          273..298
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          585..>662
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          627..660
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     CDS             1..1235
                     /gene="LOC117603362"
                     /coded_by="XM_076690163.1:1806..5513"
                     /db_xref="GeneID:117603362"
ORIGIN      
        1 mpeprakrck hcdesghrld sgspfwriql dqdlldtisa iypewfkdyt nsrastssss
       61 stsgaqlnne givagdnsvl leqkvvkgds kskskakkvd sikdkerdrk esrrdskder
      121 dagngkkggs kpspqldkaa dvagrkttag vpgsdaemae gnqsppkaev ddsplqhamd
      181 rnkeslkvkl mlrrpinqlv aqgimpplkt ppafheqrkq leraktgdll kakiqqrpgr
      241 eelvrqhile dvghvdpsla erqrmlkkar ladqlndqls hrpgpleliq knilhteepi
      301 eravkeghip fkatcegqvt kpqhpdhyit feddsqsseg apspqlesrs dvletaaasa
      361 givtvslsip ttggavvvss tspvfqqasg estiqtfael cntvvgsqqq qqqsqqqaqh
      421 nqqhastqas qtngpsttll plapapspms lgsttsslsp lsnisiaspp apppaaitpr
      481 pqpspiaasa cqrtdapgkd knrkksktks qpktrtikfh eykgppnaqk tsvssttsgl
      541 gsappgetsy elllqqqqlf lqwqlewqhk ypqiilpaaq kplslnssga sdagsisgss
      601 vnapspapsn ssnnpseqpp lrplgkledm kvsdlkvelk rrnlpvsgsk pqlierlkpf
      661 tessssnsts nsngphvthm ghilmdtpgp mtsdesssha kspgstvkde pnsprmdsph
      721 gsehddpssp pvrvsgdeii keqrkrieel qrelyksqqq lqqirqtqpt tvhaeklvlq
      781 qhiqnkmqqq qlalhlqqlq hlqqqqqqhq qqqqqqqqqq qnqqqsqqqt qqhatfqqln
      841 akaslaaflq aqpnnttail dssaltainn kknqtknsat vqipaaivfn lpkptklngs
      901 llsalvaqaq aqqqqqqqqq qqqqaiasee gkpsppqyde aakllkvkie pvqkshiksq
      961 vvddvleili kngelppsaa qdpatpttpn rqlqqnlvft apadsqthsl vftaaspisp
     1021 ispiamevsq sdcvsspapa pppppplpla tfsiqlptal qqlqqqeeit sfntdllnte
     1081 gdldtamlls pqstqqtspd pqppqevtss dnanldlkel eldletldtm dfsqlecdlg
     1141 vkmeapqelm digdmpmdmd dpdwldslmp qspptasttv snhqppptsm ssgtdiydpl
     1201 lassqdpfdl fnmedsdfkm ssdlsltwdk vdfat
//
LOCUS       XP_034178315            1231 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603362 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178315
VERSION     XP_034178315.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322424.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034178315.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1231
                     /product="uncharacterized protein LOC117603362 isoform X2"
                     /calculated_mol_wt=134128
     Region          228..250
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          273..298
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          585..>662
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          627..660
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     CDS             1..1231
                     /gene="LOC117603362"
                     /coded_by="XM_034322424.2:1799..5494"
                     /db_xref="GeneID:117603362"
ORIGIN      
        1 mpeprakrck hcdesghrld sgspfwriql dqdlldtisa iypewfkdyt nsrastssss
       61 stsgaqlnne givagdnsvl leqkvvkgds kskskakkvd sikdkerdrk esrrdskder
      121 dagngkkggs kpspqldkaa dvagrkttag vpgsdaemae gnqsppkaev ddsplqhamd
      181 rnkeslkvkl mlrrpinqlv aqgimpplkt ppafheqrkq leraktgdll kakiqqrpgr
      241 eelvrqhile dvghvdpsla erqrmlkkar ladqlndqls hrpgpleliq knilhteepi
      301 eravkeghip fkatcegqvt kpqhpdhyit feddsqsseg apspqlesrs dvletaaasa
      361 givtvslsip ttggavvvss tspvfqqasg estiqtfael cntvvgsqqq qqqsqqqaqh
      421 nqqhastqas qtngpsttll plapapspms lgsttsslsp lsnisiaspp apppaaitpr
      481 pqpspiaasa cqrtdapgkd knrkksktks qpktrtikfh eykgppnaqk tsvssttsgl
      541 gsappgetsy elllqqqqlf lqwqlewqhk ypqiilpaaq kplslnssga sdagsisgss
      601 vnapspapsn ssnnpseqpp lrplgkledm kvsdlkvelk rrnlpvsgsk pqlierlkpf
      661 tessssnsts nsngphvthm ghilmdtpgp mtsdesssha kspgstvkde pnsprmdsph
      721 gsehddpssp pgdeiikeqr krieelqrel yksqqqlqqi rqtqpttvha eklvlqqhiq
      781 nkmqqqqlal hlqqlqhlqq qqqqhqqqqq qqqqqqqnqq qsqqqtqqha tfqqlnakas
      841 laaflqaqpn nttaildssa ltainnkknq tknsatvqip aaivfnlpkp tklngsllsa
      901 lvaqaqaqqq qqqqqqqqqq aiaseegkps ppqydeaakl lkvkiepvqk shiksqvvdd
      961 vleiliknge lppsaaqdpa tpttpnrqlq qnlvftapad sqthslvfta aspispispi
     1021 amevsqsdcv sspapapppp pplplatfsi qlptalqqlq qqeeitsfnt dllntegdld
     1081 tamllspqst qqtspdpqpp qevtssdnan ldlkeleldl etldtmdfsq lecdlgvkme
     1141 apqelmdigd mpmdmddpdw ldslmpqspp tasttvsnhq ppptsmssgt diydpllass
     1201 qdpfdlfnme dsdfkmssdl sltwdkvdfa t
//
LOCUS       XP_076546279            1206 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603362 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546279
VERSION     XP_076546279.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690164.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1206
                     /product="uncharacterized protein LOC117603362 isoform X3"
                     /calculated_mol_wt=131654
     Region          228..250
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          273..298
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          585..>662
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          627..660
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     CDS             1..1206
                     /gene="LOC117603362"
                     /coded_by="XM_076690164.1:1800..5420"
                     /db_xref="GeneID:117603362"
ORIGIN      
        1 mpeprakrck hcdesghrld sgspfwriql dqdlldtisa iypewfkdyt nsrastssss
       61 stsgaqlnne givagdnsvl leqkvvkgds kskskakkvd sikdkerdrk esrrdskder
      121 dagngkkggs kpspqldkaa dvagrkttag vpgsdaemae gnqsppkaev ddsplqhamd
      181 rnkeslkvkl mlrrpinqlv aqgimpplkt ppafheqrkq leraktgdll kakiqqrpgr
      241 eelvrqhile dvghvdpsla erqrmlkkar ladqlndqls hrpgpleliq knilhteepi
      301 eravkeghip fkatcegqvt kpqhpdhyit feddsqsseg apspqlesrs dvletaaasa
      361 givtvslsip ttggavvvss tspvfqqasg estiqtfael cntvvgsqqq qqqsqqqaqh
      421 nqqhastqas qtngpsttll plapapspms lgsttsslsp lsnisiaspp apppaaitpr
      481 pqpspiaasa cqrtdapgkd knrkksktks qpktrtikfh eykgppnaqk tsvssttsgl
      541 gsappgetsy elllqqqqlf lqwqlewqhk ypqiilpaaq kplslnssga sdagsisgss
      601 vnapspapsn ssnnpseqpp lrplgkledm kvsdlkvelk rrnlpvsgsk pqlierlkpf
      661 tessssnsts nsngphvthm ghilmdtpgp mtsdesssha kspgstvkde pnsprmdsph
      721 gsehddpssp pvrvsgdeii keqrkrieel qrelyksqqq lqqirqtqpt tvhaeklvlq
      781 qhiqnkmqqq qlalhlqqlq hlqqqqqqhq qqqqqqqqqq qnqqqsqqqt qqhatfqqln
      841 akaslaaflq aqpnnttail dssaltainn kknqtknsat vqipaaivfn lpkptklngs
      901 llsalvaqaq aqqqqqqqqq qqqqaiasee gkpsppqyde aakllkvkie pvqkshiksq
      961 vvddvleili kngelppsaa qdpatpttpn rqlqqnlvft apadsqthsl vftaaspisp
     1021 ispiamevsq sdclqqqeei tsfntdllnt egdldtamll spqstqqtsp dpqppqevts
     1081 sdnanldlke leldletldt mdfsqlecdl gvkmeapqel mdigdmpmdm ddpdwldslm
     1141 pqspptastt vsnhqpppts mssgtdiydp llassqdpfd lfnmedsdfk mssdlsltwd
     1201 kvdfat
//
LOCUS       XP_076546280            1188 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603362 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546280
VERSION     XP_076546280.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690165.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1188
                     /product="uncharacterized protein LOC117603362 isoform X4"
                     /calculated_mol_wt=129390
     Region          228..250
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          273..298
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          538..>615
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          580..613
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     CDS             1..1188
                     /gene="LOC117603362"
                     /coded_by="XM_076690165.1:1817..5383"
                     /db_xref="GeneID:117603362"
ORIGIN      
        1 mpeprakrck hcdesghrld sgspfwriql dqdlldtisa iypewfkdyt nsrastssss
       61 stsgaqlnne givagdnsvl leqkvvkgds kskskakkvd sikdkerdrk esrrdskder
      121 dagngkkggs kpspqldkaa dvagrkttag vpgsdaemae gnqsppkaev ddsplqhamd
      181 rnkeslkvkl mlrrpinqlv aqgimpplkt ppafheqrkq leraktgdll kakiqqrpgr
      241 eelvrqhile dvghvdpsla erqrmlkkar ladqlndqls hrpgpleliq knilhteepi
      301 eravkeghip fkatcegqvt kpqhpdhyit feddsqsseg apspqlesrs dvletaaasa
      361 givtvslsip ttggavvvss tspvfqqasg estiqtfael cntvvgsqqq qqqsqqqaqh
      421 nqqhastqas qtngpsttll plapapspms lgsttsslsp lsnisiaspp apppaaitpr
      481 pqpspiaasa cqrtdapgkd knrkksktks qpktrtikfh eykypqiilp aaqkplslns
      541 sgasdagsis gssvnapspa psnssnnpse qpplrplgkl edmkvsdlkv elkrrnlpvs
      601 gskpqlierl kpftessssn stsnsngphv thmghilmdt pgpmtsdess shakspgstv
      661 kdepnsprmd sphgsehddp ssppvrvsgd eiikeqrkri eelqrelyks qqqlqqirqt
      721 qpttvhaekl vlqqhiqnkm qqqqlalhlq qlqhlqqqqq qhqqqqqqqq qqqqnqqqsq
      781 qqtqqhatfq qlnakaslaa flqaqpnntt aildssalta innkknqtkn satvqipaai
      841 vfnlpkptkl ngsllsalva qaqaqqqqqq qqqqqqqaia seegkpsppq ydeaakllkv
      901 kiepvqkshi ksqvvddvle ilikngelpp saaqdpatpt tpnrqlqqnl vftapadsqt
      961 hslvftaasp ispispiame vsqsdcvssp apappppppl platfsiqlp talqqlqqqe
     1021 eitsfntdll ntegdldtam llspqstqqt spdpqppqev tssdnanldl keleldletl
     1081 dtmdfsqlec dlgvkmeapq elmdigdmpm dmddpdwlds lmpqspptas ttvsnhqppp
     1141 tsmssgtdiy dpllassqdp fdlfnmedsd fkmssdlslt wdkvdfat
//
LOCUS       XP_076546281            1081 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603362 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546281
VERSION     XP_076546281.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690166.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1081
                     /product="uncharacterized protein LOC117603362 isoform X5"
                     /calculated_mol_wt=118210
     Region          74..96
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          119..144
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          431..>508
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          473..506
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     CDS             1..1081
                     /gene="LOC117603362"
                     /coded_by="XM_076690166.1:15854..19099"
                     /db_xref="GeneID:117603362"
ORIGIN      
        1 mvtldteqkp erqhcvrrmc aicrrikrdk alkvklmlrr pinqlvaqgi mpplktppaf
       61 heqrkqlera ktgdllkaki qqrpgreelv rqhiledvgh vdpslaerqr mlkkarladq
      121 lndqlshrpg pleliqknil hteepierav keghipfkat cegqvtkpqh pdhyitfedd
      181 sqssegapsp qlesrsdvle taaasagivt vslsipttgg avvvsstspv fqqasgesti
      241 qtfaelcntv vgsqqqqqqs qqqaqhnqqh astqasqtng psttllplap apspmslgst
      301 tsslsplsni siasppappp aaitprpqps piaasacqrt dapgkdknrk ksktksqpkt
      361 rtikfheykg ppnaqktsvs sttsglgsap pgetsyelll qqqqlflqwq lewqhkypqi
      421 ilpaaqkpls lnssgasdag sisgssvnap spapsnssnn pseqpplrpl gkledmkvsd
      481 lkvelkrrnl pvsgskpqli erlkpftess ssnstsnsng phvthmghil mdtpgpmtsd
      541 essshakspg stvkdepnsp rmdsphgseh ddpssppvrv sgdeiikeqr krieelqrel
      601 yksqqqlqqi rqtqpttvha eklvlqqhiq nkmqqqqlal hlqqlqhlqq qqqqhqqqqq
      661 qqqqqqqnqq qsqqqtqqha tfqqlnakas laaflqaqpn nttaildssa ltainnkknq
      721 tknsatvqip aaivfnlpkp tklngsllsa lvaqaqaqqq qqqqqqqqqq aiaseegkps
      781 ppqydeaakl lkvkiepvqk shiksqvvdd vleiliknge lppsaaqdpa tpttpnrqlq
      841 qnlvftapad sqthslvfta aspispispi amevsqsdcv sspapapppp pplplatfsi
      901 qlptalqqlq qqeeitsfnt dllntegdld tamllspqst qqtspdpqpp qevtssdnan
      961 ldlkeleldl etldtmdfsq lecdlgvkme apqelmdigd mpmdmddpdw ldslmpqspp
     1021 tasttvsnhq ppptsmssgt diydpllass qdpfdlfnme dsdfkmssdl sltwdkvdfa
     1081 t
//
LOCUS       XP_076546282            1081 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603362 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546282
VERSION     XP_076546282.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690167.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1081
                     /product="uncharacterized protein LOC117603362 isoform X5"
                     /calculated_mol_wt=118210
     Region          74..96
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          119..144
                     /region_name="RPEL"
                     /note="Repeat in Drosophila CG10860, human KIAA0680 and C.
                     elegans F26H9.2; smart00707"
                     /db_xref="CDD:128947"
     Region          431..>508
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          473..506
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     CDS             1..1081
                     /gene="LOC117603362"
                     /coded_by="XM_076690167.1:5100..8345"
                     /db_xref="GeneID:117603362"
ORIGIN      
        1 mvtldteqkp erqhcvrrmc aicrrikrdk alkvklmlrr pinqlvaqgi mpplktppaf
       61 heqrkqlera ktgdllkaki qqrpgreelv rqhiledvgh vdpslaerqr mlkkarladq
      121 lndqlshrpg pleliqknil hteepierav keghipfkat cegqvtkpqh pdhyitfedd
      181 sqssegapsp qlesrsdvle taaasagivt vslsipttgg avvvsstspv fqqasgesti
      241 qtfaelcntv vgsqqqqqqs qqqaqhnqqh astqasqtng psttllplap apspmslgst
      301 tsslsplsni siasppappp aaitprpqps piaasacqrt dapgkdknrk ksktksqpkt
      361 rtikfheykg ppnaqktsvs sttsglgsap pgetsyelll qqqqlflqwq lewqhkypqi
      421 ilpaaqkpls lnssgasdag sisgssvnap spapsnssnn pseqpplrpl gkledmkvsd
      481 lkvelkrrnl pvsgskpqli erlkpftess ssnstsnsng phvthmghil mdtpgpmtsd
      541 essshakspg stvkdepnsp rmdsphgseh ddpssppvrv sgdeiikeqr krieelqrel
      601 yksqqqlqqi rqtqpttvha eklvlqqhiq nkmqqqqlal hlqqlqhlqq qqqqhqqqqq
      661 qqqqqqqnqq qsqqqtqqha tfqqlnakas laaflqaqpn nttaildssa ltainnkknq
      721 tknsatvqip aaivfnlpkp tklngsllsa lvaqaqaqqq qqqqqqqqqq aiaseegkps
      781 ppqydeaakl lkvkiepvqk shiksqvvdd vleiliknge lppsaaqdpa tpttpnrqlq
      841 qnlvftapad sqthslvfta aspispispi amevsqsdcv sspapapppp pplplatfsi
      901 qlptalqqlq qqeeitsfnt dllntegdld tamllspqst qqtspdpqpp qevtssdnan
      961 ldlkeleldl etldtmdfsq lecdlgvkme apqelmdigd mpmdmddpdw ldslmpqspp
     1021 tasttvsnhq ppptsmssgt diydpllass qdpfdlfnme dsdfkmssdl sltwdkvdfa
     1081 t
//
LOCUS       XP_034178477             271 aa            linear   INV 03-SEP-2025
DEFINITION  dexamethasone-induced Ras-related protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034178477
VERSION     XP_034178477.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322586.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..271
                     /product="dexamethasone-induced Ras-related protein 1"
                     /calculated_mol_wt=30388
     Region          1..251
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            3..10
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(5..11,53,124..125,127,155..157)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            28
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            31..33
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            50..53
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(52..53,69..70)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            124..127
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            155..157
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..271
                     /gene="LOC117603425"
                     /coded_by="XM_034322586.2:705..1520"
                     /db_xref="GeneID:117603425"
ORIGIN      
        1 mlgsarvgkt aivarflsnk feesytptie dfhrklyrir gevhqldlld tsgnhpfpam
       61 rrlsfltgdl fvvvfsldcr esfeeairlr esiletkvsa tqsatksrsk shfnlkvpmv
      121 ivgnkcdkdv ktvtveeaee ycnrqddccv fveasakrny hveelfyqlf ivaglplema
      181 pnhhrkvplt fgsptmlpps qprhkatlsi krrlsdacgv vapnvrrpsi rtdlmimrtk
      241 tcslaagnen napgsritlr ssdarktcsi q
//
LOCUS       XP_034178512             104 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603445 [Osmia lignaria lignaria].
ACCESSION   XP_034178512
VERSION     XP_034178512.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322621.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..104
                     /product="uncharacterized protein LOC117603445"
                     /calculated_mol_wt=12476
     CDS             1..104
                     /gene="LOC117603445"
                     /coded_by="XM_034322621.2:88..402"
                     /db_xref="GeneID:117603445"
ORIGIN      
        1 mvkilrdlll rpdlrkvqne plankikyfw rkgweempel mvlitptivt lfvtvhvirk
       61 qleekdklpr yyneytvfrp nnpkvprirt ddylsieepq htls
//
LOCUS       XP_034180034            1566 aa            linear   INV 03-SEP-2025
DEFINITION  lysine-specific demethylase 4C isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180034
VERSION     XP_034180034.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180034.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1566
                     /product="lysine-specific demethylase 4C isoform X1"
                     /calculated_mol_wt=175633
     Region          11..53
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          175..291
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          1261..1294
                     /region_name="PHD_JMJD2"
                     /note="PHD finger found in Jumonji domain-containing
                     protein 2 (JMJD2) family of histone demethylases; cd15493"
                     /db_xref="CDD:276968"
     Site            order(1261..1265,1269,1289)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276968"
     Region          1303..1407
                     /region_name="zf-HC5HC2H_2"
                     /note="PHD-zinc-finger like domain; pfam13832"
                     /db_xref="CDD:463991"
     Region          1417..1468
                     /region_name="Tudor_JMJD2_rpt1"
                     /note="first Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20391"
                     /db_xref="CDD:410462"
     Site            order(1449..1450,1452..1455,1457)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410462"
     Region          1472..1527
                     /region_name="Tudor_JMJD2_rpt2"
                     /note="second Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20392"
                     /db_xref="CDD:410463"
     Site            order(1483,1485,1489)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410463"
     CDS             1..1566
                     /gene="LOC117604262"
                     /coded_by="XM_034324143.2:147..4847"
                     /db_xref="GeneID:117604262"
ORIGIN      
        1 mvsnnsrgtp riqvfrptye efkdftkyve ymeskgahka glakvipppe wvprkggynl
       61 dnldltipap icqvvtgkqg lyqqiniqkk smtvkeyskl anseryntpr hfdyedlerk
      121 ywknityvap iygadvsgsl tdpdvkewni nhlgtildyv nkdygisidg vntaylyfgm
      181 wkttfawhte dmdlysinyl hfgapktwya ippehgrrle rlasgffpss yqscqaflrh
      241 kmslispqil rqysipcnki tqeageimit fpygyhagfn hgfncaestn faaprwveyg
      301 kratqctcsk dmvkismdtf vkrfqperye lwlrgedigp hpedprqtaa pmpsqmdllc
      361 snssngqlpq sylnaapknk rhtihkkkni matnpdvdme elvnrpdipp dvkkvlqdle
      421 leevddgpde qqlevlediw lkagemdvhe asvyddgynr kksrkrkkkn adkdkskskr
      481 dsnksiienv tvkteikvep ddslnyesss fpdqsieldn dshedsnviv snditgsikl
      541 edasdfseat ekpvkkkkkh sksgeqkkak sknvnktkrk hlnisifdin epldvsdadv
      601 qrklmampsl hahksltdsq srmndisesi iftdndtdts pvkvktvlni assgqvassi
      661 ysdtkgtdnt derinvytks tkktnpvsik tfgkqqsllk anvfeqgkpm kidkavesec
      721 igddtkiilq dgksssypvc ksvgtmlter sipnyskkdi ikaprlsvlk sayvsatevs
      781 pkvdperlia eendnralsn svvvlpktwp iakprepkls tpgimfvnpg fanppilqke
      841 tivkesrtds kalelsksvt pctiqipqmd siftksavla qplaqtitcq nisgntdvki
      901 lgkrsttdts kmmatkfwql ssnnsnvffa kdtkedplvy tstsldnsei sasssslglp
      961 tnsgntffft aapkytnvse kqssmttdfn krcsrnpapm snvtgsivki vpklqdhlll
     1021 ktlnqhdegc srktvkktst ivsnvksvst qvdhpleiin lnyttshsip dlqaaqrtit
     1081 ihqeqfsnvi epgdklqtqn iqrssdniya nmpslkpikl msrrkskekl kkattrkkev
     1141 etktlmpnpd eaniatcsis qspsvsnrvs mvpghisdml ypsvpnndll kafndywsaq
     1201 ishcaicapf tsscnghgrl mppdwkyckp tvlpdsspiw vsasvfvans keqvvepend
     1261 kllrcrechv tvhascygit vlptdlrnwa cdkckagktq vmcclcpmrg galkrtsdss
     1321 wahilcalll pgvtfkdain kdpinvltik pnivkqqccy cgqkdgacls cnecgnlfhp
     1381 scglvsgatf tvpvynspel qvtcdghddg kekiptirqg eivwakhrnt ryykgkvdsi
     1441 hdtlfymvtf sddsfsedly psdimnydpg nppqqgaavi vkwtdgnlyd gifggtnhri
     1501 mytvifedgs qlvlkrneiy slqedmpkrv rsrlsvatem khryhlygae deleahrkvk
     1561 qskycd
//
LOCUS       XP_034180042            1566 aa            linear   INV 03-SEP-2025
DEFINITION  lysine-specific demethylase 4C isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180042
VERSION     XP_034180042.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324151.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180042.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1566
                     /product="lysine-specific demethylase 4C isoform X1"
                     /calculated_mol_wt=175633
     Region          11..53
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          175..291
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          1261..1294
                     /region_name="PHD_JMJD2"
                     /note="PHD finger found in Jumonji domain-containing
                     protein 2 (JMJD2) family of histone demethylases; cd15493"
                     /db_xref="CDD:276968"
     Site            order(1261..1265,1269,1289)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276968"
     Region          1303..1407
                     /region_name="zf-HC5HC2H_2"
                     /note="PHD-zinc-finger like domain; pfam13832"
                     /db_xref="CDD:463991"
     Region          1417..1468
                     /region_name="Tudor_JMJD2_rpt1"
                     /note="first Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20391"
                     /db_xref="CDD:410462"
     Site            order(1449..1450,1452..1455,1457)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410462"
     Region          1472..1527
                     /region_name="Tudor_JMJD2_rpt2"
                     /note="second Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20392"
                     /db_xref="CDD:410463"
     Site            order(1483,1485,1489)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410463"
     CDS             1..1566
                     /gene="LOC117604262"
                     /coded_by="XM_034324151.2:121..4821"
                     /db_xref="GeneID:117604262"
ORIGIN      
        1 mvsnnsrgtp riqvfrptye efkdftkyve ymeskgahka glakvipppe wvprkggynl
       61 dnldltipap icqvvtgkqg lyqqiniqkk smtvkeyskl anseryntpr hfdyedlerk
      121 ywknityvap iygadvsgsl tdpdvkewni nhlgtildyv nkdygisidg vntaylyfgm
      181 wkttfawhte dmdlysinyl hfgapktwya ippehgrrle rlasgffpss yqscqaflrh
      241 kmslispqil rqysipcnki tqeageimit fpygyhagfn hgfncaestn faaprwveyg
      301 kratqctcsk dmvkismdtf vkrfqperye lwlrgedigp hpedprqtaa pmpsqmdllc
      361 snssngqlpq sylnaapknk rhtihkkkni matnpdvdme elvnrpdipp dvkkvlqdle
      421 leevddgpde qqlevlediw lkagemdvhe asvyddgynr kksrkrkkkn adkdkskskr
      481 dsnksiienv tvkteikvep ddslnyesss fpdqsieldn dshedsnviv snditgsikl
      541 edasdfseat ekpvkkkkkh sksgeqkkak sknvnktkrk hlnisifdin epldvsdadv
      601 qrklmampsl hahksltdsq srmndisesi iftdndtdts pvkvktvlni assgqvassi
      661 ysdtkgtdnt derinvytks tkktnpvsik tfgkqqsllk anvfeqgkpm kidkavesec
      721 igddtkiilq dgksssypvc ksvgtmlter sipnyskkdi ikaprlsvlk sayvsatevs
      781 pkvdperlia eendnralsn svvvlpktwp iakprepkls tpgimfvnpg fanppilqke
      841 tivkesrtds kalelsksvt pctiqipqmd siftksavla qplaqtitcq nisgntdvki
      901 lgkrsttdts kmmatkfwql ssnnsnvffa kdtkedplvy tstsldnsei sasssslglp
      961 tnsgntffft aapkytnvse kqssmttdfn krcsrnpapm snvtgsivki vpklqdhlll
     1021 ktlnqhdegc srktvkktst ivsnvksvst qvdhpleiin lnyttshsip dlqaaqrtit
     1081 ihqeqfsnvi epgdklqtqn iqrssdniya nmpslkpikl msrrkskekl kkattrkkev
     1141 etktlmpnpd eaniatcsis qspsvsnrvs mvpghisdml ypsvpnndll kafndywsaq
     1201 ishcaicapf tsscnghgrl mppdwkyckp tvlpdsspiw vsasvfvans keqvvepend
     1261 kllrcrechv tvhascygit vlptdlrnwa cdkckagktq vmcclcpmrg galkrtsdss
     1321 wahilcalll pgvtfkdain kdpinvltik pnivkqqccy cgqkdgacls cnecgnlfhp
     1381 scglvsgatf tvpvynspel qvtcdghddg kekiptirqg eivwakhrnt ryykgkvdsi
     1441 hdtlfymvtf sddsfsedly psdimnydpg nppqqgaavi vkwtdgnlyd gifggtnhri
     1501 mytvifedgs qlvlkrneiy slqedmpkrv rsrlsvatem khryhlygae deleahrkvk
     1561 qskycd
//
LOCUS       XP_034180050            1544 aa            linear   INV 03-SEP-2025
DEFINITION  lysine-specific demethylase 4C isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180050
VERSION     XP_034180050.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324159.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180050.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1544
                     /product="lysine-specific demethylase 4C isoform X2"
                     /calculated_mol_wt=173012
     Region          11..53
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          175..291
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          1261..1294
                     /region_name="PHD_JMJD2"
                     /note="PHD finger found in Jumonji domain-containing
                     protein 2 (JMJD2) family of histone demethylases; cd15493"
                     /db_xref="CDD:276968"
     Site            order(1261..1265,1269,1289)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276968"
     Region          1303..1407
                     /region_name="zf-HC5HC2H_2"
                     /note="PHD-zinc-finger like domain; pfam13832"
                     /db_xref="CDD:463991"
     Region          1417..1468
                     /region_name="Tudor_JMJD2_rpt1"
                     /note="first Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20391"
                     /db_xref="CDD:410462"
     Site            order(1449..1450,1452..1455,1457)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410462"
     Region          1472..1527
                     /region_name="Tudor_JMJD2_rpt2"
                     /note="second Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20392"
                     /db_xref="CDD:410463"
     Site            order(1483,1485,1489)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410463"
     CDS             1..1544
                     /gene="LOC117604262"
                     /coded_by="XM_034324159.2:147..4781"
                     /db_xref="GeneID:117604262"
ORIGIN      
        1 mvsnnsrgtp riqvfrptye efkdftkyve ymeskgahka glakvipppe wvprkggynl
       61 dnldltipap icqvvtgkqg lyqqiniqkk smtvkeyskl anseryntpr hfdyedlerk
      121 ywknityvap iygadvsgsl tdpdvkewni nhlgtildyv nkdygisidg vntaylyfgm
      181 wkttfawhte dmdlysinyl hfgapktwya ippehgrrle rlasgffpss yqscqaflrh
      241 kmslispqil rqysipcnki tqeageimit fpygyhagfn hgfncaestn faaprwveyg
      301 kratqctcsk dmvkismdtf vkrfqperye lwlrgedigp hpedprqtaa pmpsqmdllc
      361 snssngqlpq sylnaapknk rhtihkkkni matnpdvdme elvnrpdipp dvkkvlqdle
      421 leevddgpde qqlevlediw lkagemdvhe asvyddgynr kksrkrkkkn adkdkskskr
      481 dsnksiienv tvkteikvep ddslnyesss fpdqsieldn dshedsnviv snditgsikl
      541 edasdfseat ekpvkkkkkh sksgeqkkak sknvnktkrk hlnisifdin epldvsdadv
      601 qrklmampsl hahksltdsq srmndisesi iftdndtdts pvkvktvlni assgqvassi
      661 ysdtkgtdnt derinvytks tkktnpvsik tfgkqqsllk anvfeqgkpm kidkavesec
      721 igddtkiilq dgksssypvc ksvgtmlter sipnyskkdi ikaprlsvlk sayvsatevs
      781 pkvdperlia eendnralsn svvvlpktwp iakprepkls tpgimfvnpg fanppilqke
      841 tivkesrtds kalelsksvt pctiqipqmd siftksavla qplaqtitcq nisgntdvki
      901 lgkrsttdts kmmatkfwql ssnnsnvffa kdtkedplvy tstsldnsei sasssslglp
      961 tnsgntffft aapkytnvse kqssmttdfn krcsrnpapm snvtgsivki vpklqdhlll
     1021 ktlnqhdegc srktvkktst ivsnvksvst qvdhpleiin lnyttshsip dlqaaqrtit
     1081 ihqeqfsnvi epgdklqtqn iqrssdniya nmpslkpikl msrrkskekl kkattrkkev
     1141 etktlmpnpd eaniatcsis qspsvsnrvs mvpghisdml ypsvpnndll kafndywsaq
     1201 ishcaicapf tsscnghgrl mppdwkyckp tvlpdsspiw vsasvfvans keqvvepend
     1261 kllrcrechv tvhascygit vlptdlrnwa cdkckagktq vmcclcpmrg galkrtsdss
     1321 wahilcalll pgvtfkdain kdpinvltik pnivkqqccy cgqkdgacls cnecgnlfhp
     1381 scglvsgatf tvpvynspel qvtcdghddg kekiptirqg eivwakhrnt ryykgkvdsi
     1441 hdtlfymvtf sddsfsedly psdimnydpg nppqqgaavi vkwtdgnlyd gifggtnhri
     1501 mytvifedgs qlvlkrneiy slqedmpkrv rsrlkiiyhq srsa
//
LOCUS       XP_076546283            1536 aa            linear   INV 03-SEP-2025
DEFINITION  lysine-specific demethylase 4C isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546283
VERSION     XP_076546283.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690168.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1536
                     /product="lysine-specific demethylase 4C isoform X3"
                     /calculated_mol_wt=172119
     Region          11..53
                     /region_name="JmjN"
                     /note="Small domain found in the jumonji family of
                     transcription factors; smart00545"
                     /db_xref="CDD:128818"
     Region          175..291
                     /region_name="JmjC"
                     /note="JmjC domain, hydroxylase; pfam02373"
                     /db_xref="CDD:396791"
     Region          1261..1294
                     /region_name="PHD_JMJD2"
                     /note="PHD finger found in Jumonji domain-containing
                     protein 2 (JMJD2) family of histone demethylases; cd15493"
                     /db_xref="CDD:276968"
     Site            order(1261..1265,1269,1289)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276968"
     Region          1303..1407
                     /region_name="zf-HC5HC2H_2"
                     /note="PHD-zinc-finger like domain; pfam13832"
                     /db_xref="CDD:463991"
     Region          1417..1468
                     /region_name="Tudor_JMJD2_rpt1"
                     /note="first Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20391"
                     /db_xref="CDD:410462"
     Site            order(1449..1450,1452..1455,1457)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410462"
     Region          1472..1527
                     /region_name="Tudor_JMJD2_rpt2"
                     /note="second Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20392"
                     /db_xref="CDD:410463"
     Site            order(1483,1485,1489)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410463"
     CDS             1..1536
                     /gene="LOC117604262"
                     /coded_by="XM_076690168.1:147..4757"
                     /db_xref="GeneID:117604262"
ORIGIN      
        1 mvsnnsrgtp riqvfrptye efkdftkyve ymeskgahka glakvipppe wvprkggynl
       61 dnldltipap icqvvtgkqg lyqqiniqkk smtvkeyskl anseryntpr hfdyedlerk
      121 ywknityvap iygadvsgsl tdpdvkewni nhlgtildyv nkdygisidg vntaylyfgm
      181 wkttfawhte dmdlysinyl hfgapktwya ippehgrrle rlasgffpss yqscqaflrh
      241 kmslispqil rqysipcnki tqeageimit fpygyhagfn hgfncaestn faaprwveyg
      301 kratqctcsk dmvkismdtf vkrfqperye lwlrgedigp hpedprqtaa pmpsqmdllc
      361 snssngqlpq sylnaapknk rhtihkkkni matnpdvdme elvnrpdipp dvkkvlqdle
      421 leevddgpde qqlevlediw lkagemdvhe asvyddgynr kksrkrkkkn adkdkskskr
      481 dsnksiienv tvkteikvep ddslnyesss fpdqsieldn dshedsnviv snditgsikl
      541 edasdfseat ekpvkkkkkh sksgeqkkak sknvnktkrk hlnisifdin epldvsdadv
      601 qrklmampsl hahksltdsq srmndisesi iftdndtdts pvkvktvlni assgqvassi
      661 ysdtkgtdnt derinvytks tkktnpvsik tfgkqqsllk anvfeqgkpm kidkavesec
      721 igddtkiilq dgksssypvc ksvgtmlter sipnyskkdi ikaprlsvlk sayvsatevs
      781 pkvdperlia eendnralsn svvvlpktwp iakprepkls tpgimfvnpg fanppilqke
      841 tivkesrtds kalelsksvt pctiqipqmd siftksavla qplaqtitcq nisgntdvki
      901 lgkrsttdts kmmatkfwql ssnnsnvffa kdtkedplvy tstsldnsei sasssslglp
      961 tnsgntffft aapkytnvse kqssmttdfn krcsrnpapm snvtgsivki vpklqdhlll
     1021 ktlnqhdegc srktvkktst ivsnvksvst qvdhpleiin lnyttshsip dlqaaqrtit
     1081 ihqeqfsnvi epgdklqtqn iqrssdniya nmpslkpikl msrrkskekl kkattrkkev
     1141 etktlmpnpd eaniatcsis qspsvsnrvs mvpghisdml ypsvpnndll kafndywsaq
     1201 ishcaicapf tsscnghgrl mppdwkyckp tvlpdsspiw vsasvfvans keqvvepend
     1261 kllrcrechv tvhascygit vlptdlrnwa cdkckagktq vmcclcpmrg galkrtsdss
     1321 wahilcalll pgvtfkdain kdpinvltik pnivkqqccy cgqkdgacls cnecgnlfhp
     1381 scglvsgatf tvpvynspel qvtcdghddg kekiptirqg eivwakhrnt ryykgkvdsi
     1441 hdtlfymvtf sddsfsedly psdimnydpg nppqqgaavi vkwtdgnlyd gifggtnhri
     1501 mytvifedgs qlvlkrneiy slqedmpkrv rsrlyq
//
LOCUS       XP_034180057            1276 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604262 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180057
VERSION     XP_034180057.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324166.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180057.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1276
                     /product="uncharacterized protein LOC117604262 isoform X4"
                     /calculated_mol_wt=142179
     Region          971..1004
                     /region_name="PHD_JMJD2"
                     /note="PHD finger found in Jumonji domain-containing
                     protein 2 (JMJD2) family of histone demethylases; cd15493"
                     /db_xref="CDD:276968"
     Site            order(971..975,979,999)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276968"
     Region          1013..1117
                     /region_name="zf-HC5HC2H_2"
                     /note="PHD-zinc-finger like domain; pfam13832"
                     /db_xref="CDD:463991"
     Region          1127..1178
                     /region_name="Tudor_JMJD2_rpt1"
                     /note="first Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20391"
                     /db_xref="CDD:410462"
     Site            order(1159..1160,1162..1165,1167)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410462"
     Region          1182..1237
                     /region_name="Tudor_JMJD2_rpt2"
                     /note="second Tudor domain found in Jumonji
                     domain-containing protein 2 (JMJD2) family of histone
                     demethylases; cd20392"
                     /db_xref="CDD:410463"
     Site            order(1193,1195,1199)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410463"
     CDS             1..1276
                     /gene="LOC117604262"
                     /coded_by="XM_034324166.2:261..4091"
                     /db_xref="GeneID:117604262"
ORIGIN      
        1 mvkglhnvhv vkiwlkyqwt llssvfsqkg negkllerye lwlrgedigp hpedprqtaa
       61 pmpsqmdllc snssngqlpq sylnaapknk rhtihkkkni matnpdvdme elvnrpdipp
      121 dvkkvlqdle leevddgpde qqlevlediw lkagemdvhe asvyddgynr kksrkrkkkn
      181 adkdkskskr dsnksiienv tvkteikvep ddslnyesss fpdqsieldn dshedsnviv
      241 snditgsikl edasdfseat ekpvkkkkkh sksgeqkkak sknvnktkrk hlnisifdin
      301 epldvsdadv qrklmampsl hahksltdsq srmndisesi iftdndtdts pvkvktvlni
      361 assgqvassi ysdtkgtdnt derinvytks tkktnpvsik tfgkqqsllk anvfeqgkpm
      421 kidkavesec igddtkiilq dgksssypvc ksvgtmlter sipnyskkdi ikaprlsvlk
      481 sayvsatevs pkvdperlia eendnralsn svvvlpktwp iakprepkls tpgimfvnpg
      541 fanppilqke tivkesrtds kalelsksvt pctiqipqmd siftksavla qplaqtitcq
      601 nisgntdvki lgkrsttdts kmmatkfwql ssnnsnvffa kdtkedplvy tstsldnsei
      661 sasssslglp tnsgntffft aapkytnvse kqssmttdfn krcsrnpapm snvtgsivki
      721 vpklqdhlll ktlnqhdegc srktvkktst ivsnvksvst qvdhpleiin lnyttshsip
      781 dlqaaqrtit ihqeqfsnvi epgdklqtqn iqrssdniya nmpslkpikl msrrkskekl
      841 kkattrkkev etktlmpnpd eaniatcsis qspsvsnrvs mvpghisdml ypsvpnndll
      901 kafndywsaq ishcaicapf tsscnghgrl mppdwkyckp tvlpdsspiw vsasvfvans
      961 keqvvepend kllrcrechv tvhascygit vlptdlrnwa cdkckagktq vmcclcpmrg
     1021 galkrtsdss wahilcalll pgvtfkdain kdpinvltik pnivkqqccy cgqkdgacls
     1081 cnecgnlfhp scglvsgatf tvpvynspel qvtcdghddg kekiptirqg eivwakhrnt
     1141 ryykgkvdsi hdtlfymvtf sddsfsedly psdimnydpg nppqqgaavi vkwtdgnlyd
     1201 gifggtnhri mytvifedgs qlvlkrneiy slqedmpkrv rsrlsvatem khryhlygae
     1261 deleahrkvk qskycd
//
LOCUS       XP_034180071             641 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034180071
VERSION     XP_034180071.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324180.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="ATP-binding cassette sub-family G member 4"
                     /calculated_mol_wt=71716
     Region          60..637
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..641
                     /gene="LOC117604287"
                     /coded_by="XM_034324180.2:402..2327"
                     /db_xref="GeneID:117604287"
ORIGIN      
        1 mevlmheass gqetnptgek iyntvkepvk etqengrkns ttrfmetnpa dtvdimfeni
       61 sykvslgcrk gqkeilhgin grlpskrlia lmgpsgagks tlldvlsgyr itgvtgnvhi
      121 ngrvrqmnsf rkcsayitqd drleplltti enmrvaadlk lptsipryek etiieeilvt
      181 lglyehmntr aerlsggqrk rlsialelvn nptvmfldep ttgldsssct qvvnllklla
      241 rqgrtiicti hqpsasifql fdlvyviakg eclyhgatsq llpylesikv pcpmyhnpad
      301 yvielscgey gedkipllls gsengknlrw fenpntlida nglralhplk knggdnslqa
      361 tslthqikil lgrgflmskr dttlthlril vnifvglils svfvetgtdg srvldnynll
      421 fsilihqlmt tmmltvvtfp mqmsillkeh fnrwyslkaf ytattlidip isilcclift
      481 livyfitsqp leitrfsmff ainllvvfig qgiglmigav cnvvngtfig ptlsvplmmf
      541 sgfgvslrdi pgylkwgtyi sclryglegf isaiygydrp vlackektvg ychykypskf
      601 lsdiamegdq fwndimalit iliitrstay fllkwkiisi r
//
LOCUS       XP_034180098             641 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180098
VERSION     XP_034180098.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324207.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180098.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="ATP-binding cassette sub-family G member 1
                     isoform X1"
                     /calculated_mol_wt=72680
     Region          46..639
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..641
                     /gene="LOC117604301"
                     /coded_by="XM_034324207.2:377..2302"
                     /db_xref="GeneID:117604301"
ORIGIN      
        1 mgtkvliemq qqlrhavsss enapsstkkv ddqmdidnsi flvfedisyk arpwifsrek
       61 tellknlsge frageltaim glsgagkstl mdvltgfita dvtgnimvns kprnlnefrr
      121 fsayimqndn lqplltvqea mnvaaelklt aspqqkkqki eqilitmsld tcrhtrtgql
      181 sggerkrlai alelincppi lfldeptsgl dsvtskycit llkqlaksgq tvicsihqps
      241 asllnmvdhl yviadgscvy tgstqnlvpy lsglgfqcpt hynpadylme icngdygrhv
      301 sklinaieng knnawrstsn vttanhqeev ialnvmasfq alrqrsamet dyarrrrlts
      361 eyatnfwkql avllkrnaik lsrdkvltft rlsmhfiial ivgtiyfrig qdavyvldnf
      421 nllffnvmfl mfsafsatvt tfplelpiim rehfnrwykl hsfylankla dipiqftais
      481 lyilivyyms nqlfelkrfc lytvmccais lvaqtigllv gtgmkvqhgm ifgplmilpf
      541 lifsgffvqf rdahpwlrwi fhlsflkygf egvmiaiygy nrpklscsdv ychfaipetl
      601 lnamdmkqad ywfslivlag lyvildlttf tllkfksekr v
//
LOCUS       XP_034180107             641 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180107
VERSION     XP_034180107.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324216.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180107.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="ATP-binding cassette sub-family G member 1
                     isoform X1"
                     /calculated_mol_wt=72680
     Region          46..639
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..641
                     /gene="LOC117604301"
                     /coded_by="XM_034324216.2:248..2173"
                     /db_xref="GeneID:117604301"
ORIGIN      
        1 mgtkvliemq qqlrhavsss enapsstkkv ddqmdidnsi flvfedisyk arpwifsrek
       61 tellknlsge frageltaim glsgagkstl mdvltgfita dvtgnimvns kprnlnefrr
      121 fsayimqndn lqplltvqea mnvaaelklt aspqqkkqki eqilitmsld tcrhtrtgql
      181 sggerkrlai alelincppi lfldeptsgl dsvtskycit llkqlaksgq tvicsihqps
      241 asllnmvdhl yviadgscvy tgstqnlvpy lsglgfqcpt hynpadylme icngdygrhv
      301 sklinaieng knnawrstsn vttanhqeev ialnvmasfq alrqrsamet dyarrrrlts
      361 eyatnfwkql avllkrnaik lsrdkvltft rlsmhfiial ivgtiyfrig qdavyvldnf
      421 nllffnvmfl mfsafsatvt tfplelpiim rehfnrwykl hsfylankla dipiqftais
      481 lyilivyyms nqlfelkrfc lytvmccais lvaqtigllv gtgmkvqhgm ifgplmilpf
      541 lifsgffvqf rdahpwlrwi fhlsflkygf egvmiaiygy nrpklscsdv ychfaipetl
      601 lnamdmkqad ywfslivlag lyvildlttf tllkfksekr v
//
LOCUS       XP_034180116             641 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180116
VERSION     XP_034180116.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324225.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180116.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="ATP-binding cassette sub-family G member 1
                     isoform X1"
                     /calculated_mol_wt=72680
     Region          46..639
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..641
                     /gene="LOC117604301"
                     /coded_by="XM_034324225.2:269..2194"
                     /db_xref="GeneID:117604301"
ORIGIN      
        1 mgtkvliemq qqlrhavsss enapsstkkv ddqmdidnsi flvfedisyk arpwifsrek
       61 tellknlsge frageltaim glsgagkstl mdvltgfita dvtgnimvns kprnlnefrr
      121 fsayimqndn lqplltvqea mnvaaelklt aspqqkkqki eqilitmsld tcrhtrtgql
      181 sggerkrlai alelincppi lfldeptsgl dsvtskycit llkqlaksgq tvicsihqps
      241 asllnmvdhl yviadgscvy tgstqnlvpy lsglgfqcpt hynpadylme icngdygrhv
      301 sklinaieng knnawrstsn vttanhqeev ialnvmasfq alrqrsamet dyarrrrlts
      361 eyatnfwkql avllkrnaik lsrdkvltft rlsmhfiial ivgtiyfrig qdavyvldnf
      421 nllffnvmfl mfsafsatvt tfplelpiim rehfnrwykl hsfylankla dipiqftais
      481 lyilivyyms nqlfelkrfc lytvmccais lvaqtigllv gtgmkvqhgm ifgplmilpf
      541 lifsgffvqf rdahpwlrwi fhlsflkygf egvmiaiygy nrpklscsdv ychfaipetl
      601 lnamdmkqad ywfslivlag lyvildlttf tllkfksekr v
//
LOCUS       XP_034180138             562 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 4 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180138
VERSION     XP_034180138.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324247.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180138.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..562
                     /product="ATP-binding cassette sub-family G member 4
                     isoform X2"
                     /calculated_mol_wt=63707
     Region          1..560
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..562
                     /gene="LOC117604301"
                     /coded_by="XM_034324247.2:201..1889"
                     /db_xref="GeneID:117604301"
ORIGIN      
        1 mglsgagkst lmdvltgfit advtgnimvn skprnlnefr rfsayimqnd nlqplltvqe
       61 amnvaaelkl taspqqkkqk ieqilitmsl dtcrhtrtgq lsggerkrla ialelincpp
      121 ilfldeptsg ldsvtskyci tllkqlaksg qtvicsihqp sasllnmvdh lyviadgscv
      181 ytgstqnlvp ylsglgfqcp thynpadylm eicngdygrh vsklinaien gknnawrsts
      241 nvttanhqee vialnvmasf qalrqrsame tdyarrrrlt seyatnfwkq lavllkrnai
      301 klsrdkvltf trlsmhfiia livgtiyfri gqdavyvldn fnllffnvmf lmfsafsatv
      361 ttfplelpii mrehfnrwyk lhsfylankl adipiqftai slyilivyym snqlfelkrf
      421 clytvmccai slvaqtigll vgtgmkvqhg mifgplmilp flifsgffvq frdahpwlrw
      481 ifhlsflkyg fegvmiaiyg ynrpklscsd vychfaipet llnamdmkqa dywfslivla
      541 glyvildltt ftllkfksek rv
//
LOCUS       XP_034180151             631 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180151
VERSION     XP_034180151.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324260.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180151.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="ATP-binding cassette sub-family G member 1
                     isoform X1"
                     /calculated_mol_wt=70626
     Region          23..100
                     /region_name="FNR_like"
                     /note="Ferredoxin reductase (FNR), an FAD and NAD(P)
                     binding protein, was intially identified as a chloroplast
                     reductase activity, catalyzing the electron transfer from
                     reduced iron-sulfur protein ferredoxin to NADP+ as the
                     final step in the electron transport...; cl06868"
                     /db_xref="CDD:447143"
     Region          72..630
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..631
                     /gene="LOC117604318"
                     /coded_by="XM_034324260.2:380..2275"
                     /db_xref="GeneID:117604318"
ORIGIN      
        1 mttsphcsps rsslsccqid hsshddvhgv tiaakddyff qekhsfpkrp evdlaffdvr
       61 ytvrewslrn irpnykeilh gvsgefkage lvaimgpsga gkstllnvla gytvkgvqge
      121 ilvngkvrgp yserwkrtsc yiqqdalmrt rltvgeamtl aahlklgyti ssaykhtqvl
      181 elleilglsh cydtlcgkls ggqkkrldia lellsnpsvl fldepttgld ssscsqcial
      241 lkrlakierr tvictihqps alllemfdai ytvaagyciy rgpvksllph mssigvdcpp
      301 yhnpadflle vaigdygisv eklaaaveti skdeksityt mksqldestk eppppagfla
      361 qcyllykrql lclkrdytll vvrllchlli gvifgylymg sgyrangvla nyvylygsll
      421 livytgkmav tlafpqemri lsrehfnrwy rlapyyfsml lveipfqasc aatylavsyw
      481 ltgqpvetpr iisfmvvsia asltaqawgf figattpvki avfagpiiav lfsvfgfcir
      541 yidtpqmfrw mfhisyfras fhsllhtvyg fgrmdlkcdd fychykkptq flkemdiadt
      601 svvnnlilil gigvlmhllt asalwcklnr r
//
LOCUS       XP_034180160             631 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180160
VERSION     XP_034180160.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324269.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180160.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="ATP-binding cassette sub-family G member 1
                     isoform X1"
                     /calculated_mol_wt=70626
     Region          23..100
                     /region_name="FNR_like"
                     /note="Ferredoxin reductase (FNR), an FAD and NAD(P)
                     binding protein, was intially identified as a chloroplast
                     reductase activity, catalyzing the electron transfer from
                     reduced iron-sulfur protein ferredoxin to NADP+ as the
                     final step in the electron transport...; cl06868"
                     /db_xref="CDD:447143"
     Region          72..630
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..631
                     /gene="LOC117604318"
                     /coded_by="XM_034324269.2:527..2422"
                     /db_xref="GeneID:117604318"
ORIGIN      
        1 mttsphcsps rsslsccqid hsshddvhgv tiaakddyff qekhsfpkrp evdlaffdvr
       61 ytvrewslrn irpnykeilh gvsgefkage lvaimgpsga gkstllnvla gytvkgvqge
      121 ilvngkvrgp yserwkrtsc yiqqdalmrt rltvgeamtl aahlklgyti ssaykhtqvl
      181 elleilglsh cydtlcgkls ggqkkrldia lellsnpsvl fldepttgld ssscsqcial
      241 lkrlakierr tvictihqps alllemfdai ytvaagyciy rgpvksllph mssigvdcpp
      301 yhnpadflle vaigdygisv eklaaaveti skdeksityt mksqldestk eppppagfla
      361 qcyllykrql lclkrdytll vvrllchlli gvifgylymg sgyrangvla nyvylygsll
      421 livytgkmav tlafpqemri lsrehfnrwy rlapyyfsml lveipfqasc aatylavsyw
      481 ltgqpvetpr iisfmvvsia asltaqawgf figattpvki avfagpiiav lfsvfgfcir
      541 yidtpqmfrw mfhisyfras fhsllhtvyg fgrmdlkcdd fychykkptq flkemdiadt
      601 svvnnlilil gigvlmhllt asalwcklnr r
//
LOCUS       XP_076546284             584 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546284
VERSION     XP_076546284.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690169.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..584
                     /product="ATP-binding cassette sub-family G member 1
                     isoform X2"
                     /calculated_mol_wt=65119
     Region          28..583
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..584
                     /gene="LOC117604318"
                     /coded_by="XM_076690169.1:177..1931"
                     /db_xref="GeneID:117604318"
ORIGIN      
        1 mlrgkrnptp pphrnssklt vlvltnyyke ilhgvsgefk agelvaimgp sgagkstlln
       61 vlagytvkgv qgeilvngkv rgpyserwkr tscyiqqdal mrtrltvgea mtlaahlklg
      121 ytissaykht qvlelleilg lshcydtlcg klsggqkkrl dialellsnp svlfldeptt
      181 gldssscsqc iallkrlaki errtvictih qpsalllemf daiytvaagy ciyrgpvksl
      241 lphmssigvd cppyhnpadf llevaigdyg isveklaaav etiskdeksi tytmksqlde
      301 stkeppppag flaqcyllyk rqllclkrdy tllvvrllch lligvifgyl ymgsgyrang
      361 vlanyvylyg slllivytgk mavtlafpqe mrilsrehfn rwyrlapyyf smllveipfq
      421 ascaatylav sywltgqpve tpriisfmvv siaasltaqa wgffigattp vkiavfagpi
      481 iavlfsvfgf ciryidtpqm frwmfhisyf rasfhsllht vygfgrmdlk cddfychykk
      541 ptqflkemdi adtsvvnnli lilgigvlmh lltasalwck lnrr
//
LOCUS       XP_034180174             537 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette subfamily G member 4 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180174
VERSION     XP_034180174.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324283.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180174.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..537
                     /product="ATP-binding cassette subfamily G member 4
                     isoform X3"
                     /calculated_mol_wt=59904
     Region          1..536
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..537
                     /gene="LOC117604318"
                     /coded_by="XM_034324283.2:241..1854"
                     /db_xref="GeneID:117604318"
ORIGIN      
        1 mgpsgagkst llnvlagytv kgvqgeilvn gkvrgpyser wkrtscyiqq dalmrtrltv
       61 geamtlaahl klgytissay khtqvlelle ilglshcydt lcgklsggqk krldialell
      121 snpsvlflde pttgldsssc sqciallkrl akierrtvic tihqpsalll emfdaiytva
      181 agyciyrgpv ksllphmssi gvdcppyhnp adfllevaig dygisvekla aavetiskde
      241 ksitytmksq ldestkeppp pagflaqcyl lykrqllclk rdytllvvrl lchlligvif
      301 gylymgsgyr angvlanyvy lygslllivy tgkmavtlaf pqemrilsre hfnrwyrlap
      361 yyfsmllvei pfqascaaty lavsywltgq pvetpriisf mvvsiaaslt aqawgffiga
      421 ttpvkiavfa gpiiavlfsv fgfciryidt pqmfrwmfhi syfrasfhsl lhtvygfgrm
      481 dlkcddfych ykkptqflke mdiadtsvvn nlililgigv lmhlltasal wcklnrr
//
LOCUS       XP_034180185             620 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 4 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180185
VERSION     XP_034180185.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324294.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..620
                     /product="ATP-binding cassette sub-family G member 4
                     isoform X1"
                     /calculated_mol_wt=70420
     Region          54..615
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..620
                     /gene="LOC117604340"
                     /coded_by="XM_034324294.2:746..2608"
                     /db_xref="GeneID:117604340"
ORIGIN      
        1 memdrddaiv dptvrmideg csihlrdnen fvnpqgrpvs qmhvefddls ysicyskgkr
       61 kkimenvtgc frperlttiv gpsgagkttl lriistlkss nvqgsitvng aewnggafrk
      121 qscylpqefa lspllttret lyiaarlkmr gnldrrafcl ivteiaesln ltncldtmve
      181 dlsggerkrl aigveiitrp svllldepts gldstssyqv icllhkiarg gctvvcaihq
      241 pssrmisqfd dllvlhkgrs yycgkwddvl stfndagytc pqfynisefv levvtkergg
      301 dlnslykinr ekylqwkthi qkpkdvttat kleclnnksk qsriwqeqki lflramicik
      361 rdntltklrf aahlvvgfll gvvfynsgia anrvnsniac iffillflyf snsmpavqif
      421 pieaavfvre ylnnwyhlrs yyfvkvisdl plqilnpstf iaiayymtgq pmeshrffrt
      481 wlicvlttil gqssgmlvgv tfntnlgvfl ipavnmpiil fagfflkfse vtlylqplca
      541 isffryafeg ilqaiydggr erlqcdeiyc hlrspkqilk mmdmpsvyys ttvialciwi
      601 vclqvctyli lkwkahiakr
//
LOCUS       XP_034180193             557 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-binding cassette sub-family G member 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034180193
VERSION     XP_034180193.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324302.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="ATP-binding cassette sub-family G member 1
                     isoform X2"
                     /calculated_mol_wt=63106
     Region          3..552
                     /region_name="3a01204"
                     /note="The Eye Pigment Precursor Transporter (EPP) Family
                     protein; TIGR00955"
                     /db_xref="CDD:273361"
     CDS             1..557
                     /gene="LOC117604340"
                     /coded_by="XM_034324302.2:189..1862"
                     /db_xref="GeneID:117604340"
ORIGIN      
        1 menvtgcfrp erlttivgps gagkttllri istlkssnvq gsitvngaew nggafrkqsc
       61 ylpqefalsp llttretlyi aarlkmrgnl drrafclivt eiaeslnltn cldtmvedls
      121 ggerkrlaig veiitrpsvl lldeptsgld stssyqvicl lhkiarggct vvcaihqpss
      181 rmisqfddll vlhkgrsyyc gkwddvlstf ndagytcpqf ynisefvlev vtkerggdln
      241 slykinreky lqwkthiqkp kdvttatkle clnnkskqsr iwqeqkilfl ramicikrdn
      301 tltklrfaah lvvgfllgvv fynsgiaanr vnsniaciff illflyfsns mpavqifpie
      361 aavfvreyln nwyhlrsyyf vkvisdlplq ilnpstfiai ayymtgqpme shrffrtwli
      421 cvlttilgqs sgmlvgvtfn tnlgvflipa vnmpiilfag fflkfsevtl ylqplcaisf
      481 fryafegilq aiydggrerl qcdeiychlr spkqilkmmd mpsvyysttv ialciwivcl
      541 qvctylilkw kahiakr
//
LOCUS       XP_076546285             372 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305675 [Osmia lignaria lignaria].
ACCESSION   XP_076546285
VERSION     XP_076546285.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690170.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..372
                     /product="uncharacterized protein LOC143305675"
                     /calculated_mol_wt=40417
     Region          36..175
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..372
                     /gene="LOC143305675"
                     /coded_by="XM_076690170.1:1..1119"
                     /db_xref="GeneID:143305675"
ORIGIN      
        1 mqltqpaqtt paattdaptq ssaprsrlpe ialptfegdy skwaefkahf vsviceradl
       61 tapdkflylk gavtgsaanl ishlpttdga ldqawqlldd rydnkrlnvq shmdrlanlk
      121 pmklrkaasl lkmvniiqet qqalrsfgle dkhncflltm lvrlldadtr arwesslatq
      181 tdypsleelt tfllarartl eqleqisaar atqtttvpak ttpptqpthq rpashraavh
      241 agtarpaapv haapaghars tqsalypcaf cqkdhfvsay pvfraltphd ranvvkahal
      301 cvnclghhnl rscrttqrck iceeahhtml hgadisrvlv pgpapaaaaq ttptpapaaa
      361 tqttqttstt th
//
LOCUS       XP_076546286            1305 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611217 [Osmia lignaria lignaria].
ACCESSION   XP_076546286
VERSION     XP_076546286.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690171.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1305
                     /product="uncharacterized protein LOC117611217"
                     /calculated_mol_wt=147042
     Region          23..162
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            order(27,29,31,54..56)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            27..29
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            27
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(54..57,61..64)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          300..513
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(364..369,403..404,439,441..442,509..510)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(364..369,403,441)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            404
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          529..689
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          913..965
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1194..1287
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1305
                     /gene="LOC117611217"
                     /coded_by="XM_076690171.1:1..3918"
                     /db_xref="GeneID:117611217"
ORIGIN      
        1 mhpqpsqpth akiiqptppq qvrilvdsgs evsliseslv nqlrlrrqrs sldvhgvgga
       61 rtsqtngvvn ialhsnyrpl svtvqahvlt kvttclpsep ptrptlpshl erlnladpqf
      121 lvsrpvdiii gadaygqvik pniirnastp liaqlsifgw lvigplegiq tirrtshqvt
      181 vnntdrqlse llsrfwtqee ppqdtapllt peeqecedhf ktthsrdsag ryivrlllkl
      241 hpralgtsyq tahnclqrtl rrlskdaqyk qlytkfmley eqlghmirln ndsiispfqy
      301 flphhgvlkl gsattalrvv fngssptssg yslndllhtg pnlmlniadl liwirrfkhl
      361 fatdvtkmyr qikvhpedws lqqilwldet qqetqyqltt vtygtkaapy lavrtllqla
      421 ddegskypla vepvkngrcv ddifggadta dhlqdvanql tqlcqaggfp lakwhstsks
      481 llealatdqn naaisfddce tkileikwtp hedafnfati satqrtqftk rlvlsevaql
      541 fdplgfvapv virakiliqe lwlqelgwdd llpltitnrw lriredltrl arlsiprwfn
      601 tmttstvelh gfsdasvfam aavvylvvha pstgthtali csktrvtplk rltiprlelt
      661 aalllsklmr hvhatlnmtv sqtfmwtdse vtlawikthp skwkdyvrnr viqiqeitqn
      721 yhwrhvpgss npadcasrgm ttdqlqqhsl wwtgppgltq sqnawpkqts vdadelgape
      781 aravvslhta navkeyswel iykyssfhrl lritslclkf vdklfkrqda ppthfitaad
      841 merariywiq atqstffkde laalakgptl psthpftrlt ayldhqgiar vgrrlqhsel
      901 shdgkhpvil prnsklstll idhahrqtmh ggtqstlafl rqrywilggr apvkthilrc
      961 vqcarqrgir ahqlmgqlpl crvtssrpft htgvdyagpl tlktwkgrga ktqkgwicvf
     1021 vcfatsavhl evvsdystda fiaayrrftp rrgiaaalys dcgtnfqgad mqlkkqftqs
     1081 tqenqhiaal laedgtqwhf nppaaphmgg kweavvkshk fhlkrtvgda lltyeesvtl
     1141 ltqieailnl rpleplsddp ddvsalspgq fligsqinav peptlldiav nrwsrwqflq
     1201 qrlqqfwrlw stqylqrlqa iskwhhpsne ikvgtlvlit derlppgkwp marvlnllpg
     1261 kdgltrvvel ktatttltrp iaklailhqp sqdssdpqts sptsr
//
LOCUS       XP_034194793             609 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611014 [Osmia lignaria lignaria].
ACCESSION   XP_034194793
VERSION     XP_034194793.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338902.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..609
                     /product="uncharacterized protein LOC117611014"
                     /calculated_mol_wt=68808
     Region          232..282
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          508..601
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..609
                     /gene="LOC117611014"
                     /coded_by="XM_034338902.2:1..1830"
                     /db_xref="GeneID:117611014"
ORIGIN      
        1 msgptiawtd ssvtlhwirg hasqwkpfva hrvsdiqhei padnwrhirs pdnpadlatr
       61 gmspaelvds diwwhgpkwl teqpefwpai fpesnegiea errgatvhvv kekfgenflc
      121 rfsslshlls viaqcfrfas lsrgqpcekg fvkaaertha flavlrfsqh tsfleeieql
      181 qrhgelrkas plsscrpfld dqgllrlggr lenaalpyne khpylvakrc plarllirda
      241 hyrtlhggpq ltrshlmrrv wivrgrslvr seiaacvtca rfsgrragql mgqlpqerta
      301 aqraflttgv dyagpiklrt spgrghkayk gyialfvcls traihleavs dltsasflaa
      361 frrfvgrrgh catlfsdngt vfqgadrelr amfrqstlfy qeaaaslald gtswqfippy
      421 aphfgglwea gvksvkhhlr rvigdstltf eelttllcqi eaclnsrply alsedpsela
      481 altpghflig epttvppepe epctalhtrw rlisqmraqf wkrwrreylq hlqqfpkwrr
      541 yrenleigsl alirddlqpp akwlmgrvte lhpgpdgcvr vvtlrtekgt vkrpivklcp
      601 lpvsptdas
//
LOCUS       XP_034194794             770 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611015 [Osmia lignaria lignaria].
ACCESSION   XP_034194794
VERSION     XP_034194794.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338903.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194794.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..770
                     /product="uncharacterized protein LOC117611015"
                     /calculated_mol_wt=84823
     Region          165..328
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          453..664
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(517..522,557..558,593,595..596,660..661)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(517..522,557,595)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            558
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     Region          681..>770
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..770
                     /gene="LOC117611015"
                     /coded_by="XM_034338903.2:1..2313"
                     /db_xref="GeneID:117611015"
ORIGIN      
        1 mtdpslhavs ptsskeikpk tkggasakgv nsnvlaaask spparkcpgc sgthglgfcy
       61 kfkalspakr kervqqlkac lsclnvghev gkcpskqvcl acskrhhtll healtappas
      121 apgreggqpa redaastsdd atqqvttlsl svgskavall atakvrleap sgetvevral
      181 ldtgsdtslv sswvaqalrl prravrvais gvkdnevgha tgevslvvrp rhtsdfrlpv
      241 ralvlrklts llpskrivak swphitgltl adpeygvpar vdlllgadvc gtlfgddsrr
      301 gpegtptakl tpfgwvlmgp asddkpkaet aarvlhchge dstsqllqrf weldeirera
      361 pmsqeeekce rhfldthard psgryvvrlp fvkdprtalk snrttalkll fncerrlssd
      421 atlkeqyrnf mmeyltlqhm qgapeeadkg payylphhav rkrhdpsakl rvvfnasfct
      481 atgqslndcl atgqklqadl wmvltrwrlf rivfttdivk mfrqirvhse dtkwqsilwr
      541 atpdervqdf rlttvtygta capflalrvl aqlandessr fprgasvvrr htyvddiltg
      601 addvsetlal krevvaifka ggfelskwas nipeiqeads sntrlfqews gistlgvnwn
      661 prddafslrv aaaelvrect tkrsilseia glfdplgwaa pvlvvakilm qdlwilgadw
      721 dqqlpenvct mwqrfrgslp qldtlkiprw tfassepstq melhgfcdvs
//
LOCUS       XP_034181278             804 aa            linear   INV 03-SEP-2025
DEFINITION  AFG3 like matrix AAA peptidase subunit 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181278
VERSION     XP_034181278.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325387.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..804
                     /product="AFG3 like matrix AAA peptidase subunit 2 isoform
                     X1"
                     /calculated_mol_wt=90644
     Region          146..739
                     /region_name="HflB"
                     /note="ATP-dependent Zn proteases [Posttranslational
                     modification, protein turnover, chaperones]; COG0465"
                     /db_xref="CDD:440233"
     CDS             1..804
                     /gene="Afg3l2"
                     /coded_by="XM_034325387.2:117..2531"
                     /db_xref="GeneID:117604860"
ORIGIN      
        1 mahqllfsts klerfvlnsi ykngnifqlr rylslirtgn epttierlqk qwrlwlkppk
       61 gfekfykske snsnkttekv aekvkegssk sasksesssg rqsqkssdns lgndfkrnfq
      121 weykwdggnn pfknknnekm iifgsvtmav fylylqhvss lateitwref itkclskgvv
      181 skvevvnkqw vrvkllpght vngkdtlwfn igsvetfern lesaqielni eshnylpvhy
      241 knevelrhvl kflpqlviwg vlfflfrksm aqfgrgkkgl fgsvmestak linskdigvr
      301 fkdvagceea kieimefvnf lknpqqyvel gakipkgaml tgppgtgktl lakatagean
      361 vpfisvsgse flemfvgvgp srvrdmfama rkhapcilfi deidavgrkr ggrnfgghse
      421 qentlnqllv emdgfntttn vvvlaatnri dildkallrp grfdrqiyvs apdikgrasi
      481 fkvhlgplkt tvdkdqlark masltpgftg adianvcnea aliaardlnd niqikhfeqa
      541 ierviagmek ktnvlqpeek ktvayheagh avagwflqya dpllkvsiip rgkglgyaqy
      601 lpheqylytk eqlfdrmcma lggrvseeif fgrittgaqd dlqkvtqsay aqviqfgmne
      661 kvgnvsfqmp qqgdmtfdkp ysehtaqlid vevraliera hvhtrelltk hkdnvskvae
      721 rllkqeilsr ddmiellgpr pfpekstyeq fvegtgsfee dtslpkglqe wnktkksdke
      781 ekevppssda vsdsssqnqp hqrl
//
LOCUS       XP_034181287             803 aa            linear   INV 03-SEP-2025
DEFINITION  AFG3 like matrix AAA peptidase subunit 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181287
VERSION     XP_034181287.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325396.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..803
                     /product="AFG3 like matrix AAA peptidase subunit 2 isoform
                     X2"
                     /calculated_mol_wt=90515
     Region          145..738
                     /region_name="HflB"
                     /note="ATP-dependent Zn proteases [Posttranslational
                     modification, protein turnover, chaperones]; COG0465"
                     /db_xref="CDD:440233"
     CDS             1..803
                     /gene="Afg3l2"
                     /coded_by="XM_034325396.2:118..2529"
                     /db_xref="GeneID:117604860"
ORIGIN      
        1 mahqllfsts klerfvlnsi ykngnifqlr rylslirtgn epttierlqk qwrlwlkppk
       61 gfekfyksks nsnkttekva ekvkegssks asksesssgr qsqkssdnsl gndfkrnfqw
      121 eykwdggnnp fknknnekmi ifgsvtmavf ylylqhvssl ateitwrefi tkclskgvvs
      181 kvevvnkqwv rvkllpghtv ngkdtlwfni gsvetfernl esaqielnie shnylpvhyk
      241 nevelrhvlk flpqlviwgv lfflfrksma qfgrgkkglf gsvmestakl inskdigvrf
      301 kdvagceeak ieimefvnfl knpqqyvelg akipkgamlt gppgtgktll akatageanv
      361 pfisvsgsef lemfvgvgps rvrdmfamar khapcilfid eidavgrkrg grnfgghseq
      421 entlnqllve mdgfntttnv vvlaatnrid ildkallrpg rfdrqiyvsa pdikgrasif
      481 kvhlgplktt vdkdqlarkm asltpgftga dianvcneaa liaardlndn iqikhfeqai
      541 erviagmekk tnvlqpeekk tvayheagha vagwflqyad pllkvsiipr gkglgyaqyl
      601 pheqylytke qlfdrmcmal ggrvseeiff grittgaqdd lqkvtqsaya qviqfgmnek
      661 vgnvsfqmpq qgdmtfdkpy sehtaqlidv evralierah vhtrelltkh kdnvskvaer
      721 llkqeilsrd dmiellgprp fpekstyeqf vegtgsfeed tslpkglqew nktkksdkee
      781 kevppssdav sdsssqnqph qrl
//
LOCUS       XP_034181404             504 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604909 [Osmia lignaria lignaria].
ACCESSION   XP_034181404
VERSION     XP_034181404.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325513.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..504
                     /product="uncharacterized protein LOC117604909"
                     /calculated_mol_wt=57318
     Region          39..121
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          200..236
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          252..294
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          365..399
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..504
                     /gene="LOC117604909"
                     /coded_by="XM_034325513.2:131..1645"
                     /db_xref="GeneID:117604909"
ORIGIN      
        1 msevinqhei nqsywfkwnd yqnhlsdvvr qlldedcmvd vtlaaageri hahrivlcac
       61 stlfreilsq vnedhptiil sdisaqdiks iveftyhgev rvpvenissl ldaarslkic
      121 glieidglde gdlvtntkdf ndnseestti ineeteenvi nalqhsyeel edqqkeqite
      181 nitlhkkkkr rrdatkrdys ddmlesaind lksgqtliea stkhniprst lymrakalgi
      241 hlnasrneyp aecmkaaina viggsslqha semfsipktv lwrriqkegy qilrsemkrs
      301 ygsdkreaav kalergenlt kvalefkipk ttlfrdkarl vdegklplsf wkkrktenee
      361 lkksrleeav aackggkmsq aaasmtyhip kttiwrrlqq dgkkserlln srkqrvidpk
      421 hnsetkvqeg sdftycevss eipityiden sipedsviil taedmdelnl eegrqiivns
      481 esvqeyvpca tsieensnys qtes
//
LOCUS       XP_034181298             668 aa            linear   INV 03-SEP-2025
DEFINITION  prestin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181298
VERSION     XP_034181298.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325407.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="prestin isoform X1"
                     /calculated_mol_wt=74189
     Region          63..649
                     /region_name="PRK11660"
                     /note="putative transporter; Provisional; cl46980"
                     /db_xref="CDD:481320"
     CDS             1..668
                     /gene="LOC117604871"
                     /coded_by="XM_034325407.2:255..2261"
                     /db_xref="GeneID:117604871"
ORIGIN      
        1 mtdylsdems tnnpelmvrr pvyqqdeinq lykyvkpnkt vfqnlssrcq kfnclkavke
       61 sipligwlsk ynwkgnilgd iiagitvavm hipqglayai lgnvppivgi ymaffpvlvy
      121 lflgtsrhns mgtfalvcmm tgkvvatyst peqlqtnnna tdnhelsrnn qyspvevata
      181 vtftvamiql lmyllrlgvi aslladslvs gfttsaavhv ftsqvkdllg lknlpnrrga
      241 fkliftyvdy frnietvnwv aivlstviil ililnnflkk riarlsplpf piemlavlig
      301 tilsmylnlg etyglatvgh ipvglptpsl ppfslipnil ldsfvitmvs ytitmsmali
      361 faqklgyevd snqelmaqgv gnlvgsffsc mpftaslsrs liqqtvgght qlasliscgl
      421 lisvllwigp ffeplprcvl asiivvalng mlmkvkefkk fwnldkmdgi iwavtfitvi
      481 lldveyglma giffcigkli lfavrpytcs laqvpgteiy ldskrykgtv elpgikifhy
      541 sgslnfacrq qfrekvykva grkprkntrh eelteikqlr tlildfsalt ridlagtttl
      601 gnliseycdi niavyivgcs gpvyemmrkc nltelggglf smfptvadav hfarcnelth
      661 tpawyssm
//
LOCUS       XP_034181308             660 aa            linear   INV 03-SEP-2025
DEFINITION  prestin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181308
VERSION     XP_034181308.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325417.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..660
                     /product="prestin isoform X2"
                     /calculated_mol_wt=73234
     Region          55..641
                     /region_name="PRK11660"
                     /note="putative transporter; Provisional; cl46980"
                     /db_xref="CDD:481320"
     CDS             1..660
                     /gene="LOC117604871"
                     /coded_by="XM_034325417.2:181..2163"
                     /db_xref="GeneID:117604871"
ORIGIN      
        1 mstnnpelmv rrpvyqqdei nqlykyvkpn ktvfqnlssr cqkfnclkav kesipligwl
       61 skynwkgnil gdiiagitva vmhipqglay ailgnvppiv giymaffpvl vylflgtsrh
      121 nsmgtfalvc mmtgkvvaty stpeqlqtnn natdnhelsr nnqyspveva tavtftvami
      181 qllmyllrlg viaslladsl vsgfttsaav hvftsqvkdl lglknlpnrr gafkliftyv
      241 dyfrnietvn wvaivlstvi ilililnnfl kkriarlspl pfpiemlavl igtilsmyln
      301 lgetyglatv ghipvglptp slppfslipn illdsfvitm vsytitmsma lifaqklgye
      361 vdsnqelmaq gvgnlvgsff scmpftasls rsliqqtvgg htqlaslisc gllisvllwi
      421 gpffeplprc vlasiivval ngmlmkvkef kkfwnldkmd giiwavtfit villdveygl
      481 magiffcigk lilfavrpyt cslaqvpgte iyldskrykg tvelpgikif hysgslnfac
      541 rqqfrekvyk vagrkprknt rheelteikq lrtlildfsa ltridlagtt tlgnliseyc
      601 diniavyivg csgpvyemmr kcnltelggg lfsmfptvad avhfarcnel thtpawyssm
//
LOCUS       XP_034181318             660 aa            linear   INV 03-SEP-2025
DEFINITION  prestin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181318
VERSION     XP_034181318.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325427.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..660
                     /product="prestin isoform X2"
                     /calculated_mol_wt=73234
     Region          55..641
                     /region_name="PRK11660"
                     /note="putative transporter; Provisional; cl46980"
                     /db_xref="CDD:481320"
     CDS             1..660
                     /gene="LOC117604871"
                     /coded_by="XM_034325427.2:336..2318"
                     /db_xref="GeneID:117604871"
ORIGIN      
        1 mstnnpelmv rrpvyqqdei nqlykyvkpn ktvfqnlssr cqkfnclkav kesipligwl
       61 skynwkgnil gdiiagitva vmhipqglay ailgnvppiv giymaffpvl vylflgtsrh
      121 nsmgtfalvc mmtgkvvaty stpeqlqtnn natdnhelsr nnqyspveva tavtftvami
      181 qllmyllrlg viaslladsl vsgfttsaav hvftsqvkdl lglknlpnrr gafkliftyv
      241 dyfrnietvn wvaivlstvi ilililnnfl kkriarlspl pfpiemlavl igtilsmyln
      301 lgetyglatv ghipvglptp slppfslipn illdsfvitm vsytitmsma lifaqklgye
      361 vdsnqelmaq gvgnlvgsff scmpftasls rsliqqtvgg htqlaslisc gllisvllwi
      421 gpffeplprc vlasiivval ngmlmkvkef kkfwnldkmd giiwavtfit villdveygl
      481 magiffcigk lilfavrpyt cslaqvpgte iyldskrykg tvelpgikif hysgslnfac
      541 rqqfrekvyk vagrkprknt rheelteikq lrtlildfsa ltridlagtt tlgnliseyc
      601 diniavyivg csgpvyemmr kcnltelggg lfsmfptvad avhfarcnel thtpawyssm
//
LOCUS       XP_034181328             660 aa            linear   INV 03-SEP-2025
DEFINITION  prestin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181328
VERSION     XP_034181328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..660
                     /product="prestin isoform X2"
                     /calculated_mol_wt=73234
     Region          55..641
                     /region_name="PRK11660"
                     /note="putative transporter; Provisional; cl46980"
                     /db_xref="CDD:481320"
     CDS             1..660
                     /gene="LOC117604871"
                     /coded_by="XM_034325437.2:254..2236"
                     /db_xref="GeneID:117604871"
ORIGIN      
        1 mstnnpelmv rrpvyqqdei nqlykyvkpn ktvfqnlssr cqkfnclkav kesipligwl
       61 skynwkgnil gdiiagitva vmhipqglay ailgnvppiv giymaffpvl vylflgtsrh
      121 nsmgtfalvc mmtgkvvaty stpeqlqtnn natdnhelsr nnqyspveva tavtftvami
      181 qllmyllrlg viaslladsl vsgfttsaav hvftsqvkdl lglknlpnrr gafkliftyv
      241 dyfrnietvn wvaivlstvi ilililnnfl kkriarlspl pfpiemlavl igtilsmyln
      301 lgetyglatv ghipvglptp slppfslipn illdsfvitm vsytitmsma lifaqklgye
      361 vdsnqelmaq gvgnlvgsff scmpftasls rsliqqtvgg htqlaslisc gllisvllwi
      421 gpffeplprc vlasiivval ngmlmkvkef kkfwnldkmd giiwavtfit villdveygl
      481 magiffcigk lilfavrpyt cslaqvpgte iyldskrykg tvelpgikif hysgslnfac
      541 rqqfrekvyk vagrkprknt rheelteikq lrtlildfsa ltridlagtt tlgnliseyc
      601 diniavyivg csgpvyemmr kcnltelggg lfsmfptvad avhfarcnel thtpawyssm
//
LOCUS       XP_076546287             606 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604889 [Osmia lignaria lignaria].
ACCESSION   XP_076546287
VERSION     XP_076546287.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690172.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..606
                     /product="uncharacterized protein LOC117604889"
                     /calculated_mol_wt=65711
     Region          455..477
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          457..477
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Region          488..508
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          512..534
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          514..534
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(519,521,523,525..526,529..530,533,547,549,553..554,
                     557..558,561,575,577,579,581..582,585..586)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          527..550
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          542..562
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          542..562
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          554..578
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          570..587
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..606
                     /gene="LOC117604889"
                     /coded_by="XM_076690172.1:2907..4727"
                     /db_xref="GeneID:117604889"
ORIGIN      
        1 mprcymvkka lcnkyissva rgfeswgrgr sppspttmqi pvspiegsva ppvaqeylsa
       61 qsqetrattg rktaeqevsr erlnepsngn atstsnassn aiivateapa ivartetttt
      121 tvitcttgss siiipaates igssventtm vtsalpftap rspsstvteq qmsvlhhlpp
      181 gepsppssta tpyhleestn hstsesrigs dcnvvvtnya ssmfkdrsaa eteaahdlle
      241 lsrslpplpp psvaigpqsv ietpatdiqe mtvyqpdqpi yqvntidlas stvythhqqq
      301 qpptasiiye psativqqtg svfiplspvq eilltystps ipctpivaqq ppqppplqqq
      361 qlqqhqpiet appltpptse cssdiennnp nsqpsqkdke vqtvteqtev kpasytydtl
      421 lvadgrsknk kiapsqknqe tepietpets kigryvccec gkqyatssnl srhkqthrsi
      481 dsqsakkcih cgkayvsmpa lamhvlthkl thscgvcgkm fsrpwllqgh lrshtgekpy
      541 gcahcgkafa drsnlrahmq thsadknyec hkchksfalk sylnkhlesa cqrendessn
      601 dvdtpq
//
LOCUS       XP_034181365             564 aa            linear   INV 03-SEP-2025
DEFINITION  carboxylic ester hydrolase [Osmia lignaria lignaria].
ACCESSION   XP_034181365
VERSION     XP_034181365.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325474.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181365.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="carboxylic ester hydrolase"
                     /calculated_mol_wt=64009
     Region          23..499
                     /region_name="Abhydrolase"
                     /note="alpha/beta hydrolases; cl21494"
                     /db_xref="CDD:473884"
     Site            order(130..132,210..212,215,370,374..375,401,453,456)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(211,342,452)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..564
                     /gene="LOC117604898"
                     /coded_by="XM_034325474.2:140..1834"
                     /db_xref="GeneID:117604898"
ORIGIN      
        1 msgatllalv sfsfcllans rrtsvvntsi gpvrgsilqt ywnrieyssf rgipyaqppv
       61 rqlrfkppvp ikpwrkvlna yqegnvcpqk eiqtdlligd enclflnvft petrfnrrlh
      121 lrpvmvwihg gayssgssnt slygpdffle ehlvvvsfny rlgplgfltl khpdatgnag
      181 lkdqnlvlkw vqtniamfgg dpkqvtifge sagstaigfh vlsersrglf lrsismsgsp
      241 lcpwayhtpe niianayhia aslnyvpksk ddllnflqra pamdlarvgs tigldflpfr
      301 ptiedpridp tnssfltecp itrysngdfy rhammmgytr devilflgpp igianiidwa
      361 kryinqmter rghmnaepmd eiallvqrty natyeetmki atdvffsgpi dltqrllakh
      421 naqypiyyyr lsyeteysmh klyenplngt shfddigyif yvhnlkapsd rrhplnrfrk
      481 kmvnmwanfa kygdptpkhl mnsnynvtwk ssgqtgmqie lnenevmhnr tvddgtetym
      541 ksldaalpvi sscvkiptih tdif
//
LOCUS       XP_034181380             539 aa            linear   INV 03-SEP-2025
DEFINITION  scratch isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181380
VERSION     XP_034181380.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..539
                     /product="scratch isoform X1"
                     /calculated_mol_wt=58181
     Region          373..395
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          375..395
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          406..423
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          430..452
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          432..452
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(437,439,441,443..444,447..448,451,465,467,471..472,
                     475..476,479,493,495,497,499..500,503..504)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          445..468
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          460..480
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          460..480
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          472..496
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          488..505
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..539
                     /gene="scrt"
                     /coded_by="XM_034325489.2:1813..3432"
                     /db_xref="GeneID:117604900"
ORIGIN      
        1 mprclmakkw kaypwpdrvd dpqeeqndps amlqehtsps htgiqqisat mekrqhrhep
       61 edeeidvvgd tdsrqvdhqq tcwgphspta gatapspppl nasgalyyhg ytheswinhe
      121 eppkyatlrs aaelarpvvp sppppaaqqe lpvvvtgssl hqphpvaptt tpsssaslsl
      181 pprkslsmcf tstgtalslp pkkkdiyrpy slqptseirt saeedlsaaq aildlsaspa
      241 apthsvfiht lsppppppqp taptapsaat agqlqpaqqq pipvsvlvpv pssqtsqlrq
      301 qeqtpqpqsp ataeanagnc sagrdgssgs sktvaytyea ffvsdgrskr rgnstngaav
      361 pdkeatqpdr pkftctecgk qyatssnlsr hkqthrslds qsakkcihcg kayvsmpala
      421 mhvlthklah scgvcgkmfs rpwllqghlr shtgekpygc ahcgkafadr snlrahmqth
      481 sadknyecsr chktfalksy lnkhlesacl rddeippqqa agsnsnnatq hqqsnnrgl
//
LOCUS       XP_076546288             539 aa            linear   INV 03-SEP-2025
DEFINITION  scratch isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546288
VERSION     XP_076546288.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690173.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..539
                     /product="scratch isoform X1"
                     /calculated_mol_wt=58181
     Region          373..395
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          375..395
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          406..423
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          430..452
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          432..452
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(437,439,441,443..444,447..448,451,465,467,471..472,
                     475..476,479,493,495,497,499..500,503..504)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          445..468
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          460..480
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          460..480
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          472..496
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          488..505
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..539
                     /gene="scrt"
                     /coded_by="XM_076690173.1:1715..3334"
                     /db_xref="GeneID:117604900"
ORIGIN      
        1 mprclmakkw kaypwpdrvd dpqeeqndps amlqehtsps htgiqqisat mekrqhrhep
       61 edeeidvvgd tdsrqvdhqq tcwgphspta gatapspppl nasgalyyhg ytheswinhe
      121 eppkyatlrs aaelarpvvp sppppaaqqe lpvvvtgssl hqphpvaptt tpsssaslsl
      181 pprkslsmcf tstgtalslp pkkkdiyrpy slqptseirt saeedlsaaq aildlsaspa
      241 apthsvfiht lsppppppqp taptapsaat agqlqpaqqq pipvsvlvpv pssqtsqlrq
      301 qeqtpqpqsp ataeanagnc sagrdgssgs sktvaytyea ffvsdgrskr rgnstngaav
      361 pdkeatqpdr pkftctecgk qyatssnlsr hkqthrslds qsakkcihcg kayvsmpala
      421 mhvlthklah scgvcgkmfs rpwllqghlr shtgekpygc ahcgkafadr snlrahmqth
      481 sadknyecsr chktfalksy lnkhlesacl rddeippqqa agsnsnnatq hqqsnnrgl
//
LOCUS       XP_034181391             538 aa            linear   INV 03-SEP-2025
DEFINITION  scratch isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181391
VERSION     XP_034181391.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325500.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..538
                     /product="scratch isoform X2"
                     /calculated_mol_wt=58094
     Region          372..394
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          374..394
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          405..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          429..451
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          431..451
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(436,438,440,442..443,446..447,450,464,466,470..471,
                     474..475,478,492,494,496,498..499,502..503)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          444..467
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          459..479
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          459..479
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          471..495
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          487..504
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..538
                     /gene="scrt"
                     /coded_by="XM_034325500.2:1815..3431"
                     /db_xref="GeneID:117604900"
ORIGIN      
        1 mprclmakkw kaypwpdrvd dpqeeqndps amlqehtsps htgiqqisat mekrqhrhep
       61 edeeidvvgd tdsrqvdhqq tcwgphspta gatapspppl nasgalyyhg ytheswinhe
      121 eppkyatlrs aaelarpvvp sppppaaqqe lpvvvtgssl hqphpvaptt tpsssaslsl
      181 pprkslsmcf tstgtalslp pkkkdiyrpy slqptseirt saeedlsaaq aildlsaspa
      241 apthsvfiht lsppppppqp taptapsaat agqlqpaqqq pipvsvlvpv pssqtsqlrq
      301 qeqtpqpqsp ataeanagnc sagrdgsgss ktvaytyeaf fvsdgrskrr gnstngaavp
      361 dkeatqpdrp kftctecgkq yatssnlsrh kqthrsldsq sakkcihcgk ayvsmpalam
      421 hvlthklahs cgvcgkmfsr pwllqghlrs htgekpygca hcgkafadrs nlrahmqths
      481 adknyecsrc hktfalksyl nkhlesaclr ddeippqqaa gsnsnnatqh qqsnnrgl
//
LOCUS       XP_034181435             371 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604923 [Osmia lignaria lignaria].
ACCESSION   XP_034181435
VERSION     XP_034181435.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325544.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..371
                     /product="uncharacterized protein LOC117604923"
                     /calculated_mol_wt=41374
     CDS             1..371
                     /gene="LOC117604923"
                     /coded_by="XM_034325544.2:127..1242"
                     /db_xref="GeneID:117604923"
ORIGIN      
        1 mrflgfcill ltigepllvl tekvllvipq qtrrslgpsw rsappryrks shdteshyhk
       61 yysppfhyhk rpvhgsvplp hggddfdqgh eqvnhvhlpy gkgishaisy gkgyipydkv
      121 knaisygpes hyrhsgsqgy khsepdyest sysssgdypp sgqlyeahhs dayfsnpdsd
      181 vsynerrnde kkfytsrsie keltansgid fngaankdql lllqqksadl fndispqpeg
      241 gvvlptgvps atiggskegi vlrdtvsmde yqqklqemtk swpqflsnaa atfnsnfhnq
      301 pvtasytiaa sstpfngwsl nfaqpkgydv kedtmepphd frtapiqttp yqtysipvnv
      361 aqhsvvqmvq g
//
LOCUS       XP_034181447             231 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604930 [Osmia lignaria lignaria].
ACCESSION   XP_034181447
VERSION     XP_034181447.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325556.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..231
                     /product="uncharacterized protein LOC117604930"
                     /calculated_mol_wt=26513
     CDS             1..231
                     /gene="LOC117604930"
                     /coded_by="XM_034325556.2:3164..3859"
                     /db_xref="GeneID:117604930"
ORIGIN      
        1 mppvrivrfl lrswwnarne rnqrcplpsf yvtvkssdft lpprrlftgw fwpprfvlyk
       61 htslavqsqk ridnrdtviv lgisfffslf lflinlvisi aprarsngqv skgrslrvnq
      121 ptkmrsqtia livfgilava lsspvpgyhg syhhvkvhvp yhvhtvhhhh vkkvpvhvve
      181 kvpvpvpipi hhvkkvavpv pvhhvekvhv pvpivekvhv pvpvheekqw w
//
LOCUS       XP_076546289             151 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604937 [Osmia lignaria lignaria].
ACCESSION   XP_076546289
VERSION     XP_076546289.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690174.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="uncharacterized protein LOC117604937"
                     /calculated_mol_wt=14257
     CDS             1..151
                     /gene="LOC117604937"
                     /coded_by="XM_076690174.1:4296..4751"
                     /db_xref="GeneID:117604937"
ORIGIN      
        1 mtklaailvv atllelasag ghhgghdhvv ihvpykikti hhthtitkhi hhgggggdky
       61 evlgytvghp idlghgggfg gghdlggggg hdfggggdfg ggggggghie ysgggggggg
      121 hieysgggdi ggggfggggy gggghedwgg h
//
LOCUS       XP_034181472              95 aa            linear   INV 03-SEP-2025
DEFINITION  signal peptidase complex subunit 1-like [Osmia lignaria lignaria].
ACCESSION   XP_034181472
VERSION     XP_034181472.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325581.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181472.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..95
                     /product="signal peptidase complex subunit 1-like"
                     /calculated_mol_wt=10850
     Region          15..84
                     /region_name="SPC12"
                     /note="Microsomal signal peptidase 12 kDa subunit (SPC12);
                     pfam06645"
                     /db_xref="CDD:461973"
     CDS             1..95
                     /gene="LOC117604945"
                     /coded_by="XM_034325581.2:115..402"
                     /db_xref="GeneID:117604945"
ORIGIN      
        1 mnlliqyiks ipthmdydgq araekfsrii vtlfgivgli wgyviqqfsq tiyilgagll
       61 msilitvpaw pmyrrkpldw qkpqltnaak lkkkk
//
LOCUS       XP_034181485              95 aa            linear   INV 03-SEP-2025
DEFINITION  signal peptidase complex subunit Spase12 [Osmia lignaria lignaria].
ACCESSION   XP_034181485
VERSION     XP_034181485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..95
                     /product="signal peptidase complex subunit Spase12"
                     /calculated_mol_wt=10944
     Region          15..85
                     /region_name="SPC12"
                     /note="Microsomal signal peptidase 12 kDa subunit (SPC12);
                     pfam06645"
                     /db_xref="CDD:461973"
     CDS             1..95
                     /gene="Spase12"
                     /coded_by="XM_034325594.2:121..408"
                     /db_xref="GeneID:117604951"
ORIGIN      
        1 msawvqyiks iptymdydgq araerlsrii iilfgvvgli wgyviqqfsq tiyilgagfv
       61 laviitvppw pmyrrkplew qkpqsevtvk qkkkk
//
LOCUS       XP_076546290             399 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604919 [Osmia lignaria lignaria].
ACCESSION   XP_076546290
VERSION     XP_076546290.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690175.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="uncharacterized protein LOC117604919"
                     /calculated_mol_wt=34563
     CDS             1..399
                     /gene="LOC117604919"
                     /coded_by="XM_076690175.1:53..1252"
                     /db_xref="GeneID:117604919"
ORIGIN      
        1 melkiavrcl ifttiftatl atfgkehvhi rihvpeivqh eekkiikyed hgghdhgggg
       61 gghgggggdq tiivtspggg ggghgggfgg ghgggggfgg gfgghggggf gggfgghggg
      121 hgggggdhai iitspggggg gggghgggfg gghgggfggg hgggfggggh gggggygggh
      181 gdviisspgg ghgggggfdd hhggggfggg ggfddhhggg gfgggggggy gggggfddhh
      241 ggggfggggg ggfddhhggg gfgggggggf sdhhggggig ggggggfddh hggggfgggg
      301 gggfddhhgg ggfggggggg gfgdhhgggg yggggggggg fgdhhggggg gyggggggfd
      361 hhsisnsiss gfgssggggf ggghggghgg gfsgyhkkk
//
LOCUS       XP_076546291             282 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305678 [Osmia lignaria lignaria].
ACCESSION   XP_076546291
VERSION     XP_076546291.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690176.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="uncharacterized protein LOC143305678"
                     /calculated_mol_wt=32240
     Region          <5..93
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     CDS             1..282
                     /gene="LOC143305678"
                     /coded_by="XM_076690176.1:1..849"
                     /db_xref="GeneID:143305678"
ORIGIN      
        1 mscevivagd fntkavewgm epdsrgrrvm emasrlglvv lnsgqtstfr rpgyrqtivd
       61 isfstealea rvhdwrvied ytssdhqyit ysvrhraprr vyaaqpryqw radradheal
      121 fhalelgkps lesiprgtdp rkkaerlasq tmahiqrasa lrgrclrlrr vaqrarrred
      181 vapqstadyk aarrelrhai rvsqrrcwqq lgddlnrdpw glgyrvvkkk lgaqvavgpm
      241 dsctmrhivd tlfpdhpkrs erqhptwepn appslaksss pp
//
LOCUS       XP_076546292              99 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601384 [Osmia lignaria lignaria].
ACCESSION   XP_076546292
VERSION     XP_076546292.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690177.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..99
                     /product="uncharacterized protein LOC117601384"
                     /calculated_mol_wt=10867
     CDS             1..99
                     /gene="LOC117601384"
                     /coded_by="XM_076690177.1:24..323"
                     /db_xref="GeneID:117601384"
ORIGIN      
        1 mkfvialfal lavahaypgi ygysepvhap evhhaiqipq qqsvppphpq plsikiphvp
       61 ivkipyhgak vitphligve qyvepvkivk apvhhqpwd
//
LOCUS       XP_076546293              99 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601384 [Osmia lignaria lignaria].
ACCESSION   XP_076546293
VERSION     XP_076546293.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690178.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..99
                     /product="uncharacterized protein LOC117601384"
                     /calculated_mol_wt=10867
     CDS             1..99
                     /gene="LOC117601384"
                     /coded_by="XM_076690178.1:24..323"
                     /db_xref="GeneID:117601384"
ORIGIN      
        1 mkfvialfal lavahaypgi ygysepvhap evhhaiqipq qqsvppphpq plsikiphvp
       61 ivkipyhgak vitphligve qyvepvkivk apvhhqpwd
//
LOCUS       XP_034181072             745 aa            linear   INV 03-SEP-2025
DEFINITION  I-kappaB kinase epsilon [Osmia lignaria lignaria].
ACCESSION   XP_034181072
VERSION     XP_034181072.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325181.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..745
                     /product="I-kappaB kinase epsilon"
                     /calculated_mol_wt=84912
     Region          18..332
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(18..21,24,26,39,41,71,89..92,138,142..143,145,
                     159..160)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          310..398
                     /region_name="TBK1_ULD"
                     /note="TANK binding kinase 1 ubiquitin-like domain;
                     pfam18396"
                     /db_xref="CDD:465744"
     Region          402..664
                     /region_name="TBK1_CCD1"
                     /note="TANK-binding kinase 1 coiled-coil domain 1;
                     pfam18394"
                     /db_xref="CDD:465742"
     CDS             1..745
                     /gene="IKKepsilon"
                     /coded_by="XM_034325181.2:1..2238"
                     /db_xref="GeneID:117604754"
ORIGIN      
        1 msflrgsanf vwcttsvlgk gatgavfqgv nknngepvav ktfnqlshmr phdvqmrefe
       61 vlkkvnheni vqllaieeeq dgrgmvivme lctggslfni lddpentygl aesefllvle
      121 hltagmkhlr dnnlvhrdlk pgnimkfiad dgstiykltd fgaarelqed qqfvslygte
      181 eylhpdiyer avlrkpvgkt fgatvdlwsi gvtfyhvatg nlpfrpfggr rnketmfyit
      241 tkkasgvisg vqtsengpie wsrelpqncq lsvglkkivt pllagllevd pqkiwsferf
      301 ftevtdtlsr krihifnihk gslikvflhp deklttlqvh iqdqtdiaph aqillfgdtf
      361 ltniveestp gkgypntsen kplmlfsken nnvnlpmdae lpkfpvfssl vsvendanqa
      421 kiacsvgyvc krrieklvlc sklsqdaiea fsghvlkela rllekcqrsk eftkaiedtt
      481 tvvercenla rqisrklgsq nnleimnwkk eldlqskell telapavtql hqryvkegsl
      541 rseweniirg lwcpwvnkas qkattlvdrl rdgwqhllrd ratrnltynd eqfhvlervk
      601 vtetgqrlkn lletfcvpsm vsrsecvadw ykmaqtvflq tqildkdvdn yehvletysy
      661 rlsqeskery esflhlvdrl pgklktvekt nlpaqentkk wknicimqer ismilyrngl
      721 lvdqidrlsa ldgventnds eytsl
//
LOCUS       XP_076546294             732 aa            linear   INV 03-SEP-2025
DEFINITION  protein VAC14 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546294
VERSION     XP_076546294.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690179.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..732
                     /product="protein VAC14 homolog isoform X1"
                     /calculated_mol_wt=82700
     Region          53..78
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          67..163
                     /region_name="Vac14_Fab1_bd"
                     /note="Vacuolar 14 Fab1-binding region; pfam12755"
                     /db_xref="CDD:403838"
     Site            order(70..71,74,77..78,111..112,115,118..119,122,152..153,
                     155,158..159,162,193..194,197,200..201,234..235,238,
                     241..242)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          94..122
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          134..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          172..201
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          214..243
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          499..675
                     /region_name="Vac14_Fig4_bd"
                     /note="Vacuolar protein 14 C-terminal Fig4p binding;
                     pfam11916"
                     /db_xref="CDD:463395"
     CDS             1..732
                     /gene="LOC117604760"
                     /coded_by="XM_076690179.1:120..2318"
                     /db_xref="GeneID:117604760"
ORIGIN      
        1 mmserdyapl stacvrslnd klyekrkpaa leiekmvkef sahnntvqik rllkvlgqdf
       61 atsqnphtrk ggliglaaia vglgkdtgqy iedlihpila cfcdsdlrvr yyaceslynv
      121 vkvargavlp qftdifaals klacdseqnv knatelldrl mkdivtesgl fdlvgfmpll
      181 reriytknaf grqfviawvs vldavpnmdf iiflpeildg lfkiledptp eikkvtdtvl
      241 geflrsikan pgrvdfpgmi nilithaqst dellqltait wikefvqlsg plmlpymsgi
      301 liavlpclay dgdtrkcike tatqvnanlm klvtmentqv vnnasendvq tqkgnvilvs
      361 ivkrkiavsi tflnflslds sqnrslaesl dlssvvevlt khlmymsvqt kvavlkwihh
      421 lfiniphkmf nhienlfpil mkslsdnsde vvqqtlvvma eiisskspka attdsnaemq
      481 nkyftkfivn llrlfstdrh lleergafii relcvllsae diyktlakil ldepnlsfac
      541 tmiqtlsvil ltsselfdlr nklkdldsle scklfkclyv swchnpvatv alcflsqiyg
      601 haydiirsfe nievtveflt eidklvqlie spiftylrlq llqredalvy alygllmilp
      661 qseayatlqr rlaaippttk pipksesssk sfkdrldfpe llkhfhtiqe qhkeqkrkqr
      721 ltnlieknng di
//
LOCUS       XP_076546295             732 aa            linear   INV 03-SEP-2025
DEFINITION  protein VAC14 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546295
VERSION     XP_076546295.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690180.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..732
                     /product="protein VAC14 homolog isoform X2"
                     /calculated_mol_wt=82531
     Region          53..78
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          67..163
                     /region_name="Vac14_Fab1_bd"
                     /note="Vacuolar 14 Fab1-binding region; pfam12755"
                     /db_xref="CDD:403838"
     Site            order(70..71,74,77..78,111..112,115,118..119,122,152..153,
                     155,158..159,162,193..194,197,200..201,234..235,238,
                     241..242)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          94..122
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          134..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          172..201
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          214..243
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          499..675
                     /region_name="Vac14_Fig4_bd"
                     /note="Vacuolar protein 14 C-terminal Fig4p binding;
                     pfam11916"
                     /db_xref="CDD:463395"
     CDS             1..732
                     /gene="LOC117604760"
                     /coded_by="XM_076690180.1:121..2319"
                     /db_xref="GeneID:117604760"
ORIGIN      
        1 mmserdyapl stacvrslnd klyekrkpaa leiekmvkef sahnntvqik rllkvlgqdf
       61 atsqnphtrk ggliglaaia vglgkdtgqy iedlihpila cfcdsdlrvr yyaceslynv
      121 vkvargavlp qftdifaals klacdseqnv knatelldrl mkdivtesgl fdlvgfmpll
      181 reriytknaf grqfviawvs vldavpnmdf iiflpeildg lfkiledptp eikkvtdtvl
      241 geflrsikan pgrvdfpgmi nilithaqst dellqltait wikefvqlsg plmlpymsgi
      301 liavlpclay dgdtrkcike tatqvnanlm klvtmentqv vnnasendvq tqkgnvilvs
      361 ivkrkiavsi tflnflslds sqnrslaesl dlssvvevlt khlmymsvqt kvavlkwihh
      421 lfiniphkmf nhienlfpil mkslsdnsde vvqqtlvvma eiisskspka attdsnaemq
      481 nkyftkfivn llrlfstdrh lleergafii relcvllsae diyktlakil ldepnlsfac
      541 tmiqtlsvil ltsselfdlr nklkdldsle scklfkclyv swchnpvatv alcflsqiyg
      601 haydiirsfe nievtveflt eidklvqlie spiftylrlq llqredalvy alygllmilp
      661 qseayatlqr rlaaippttk pipksesssk sfkdrldfpe llkhfhtiqe qhkeqklfsf
      721 pgslstkckk ls
//
LOCUS       XP_076546296             732 aa            linear   INV 03-SEP-2025
DEFINITION  protein VAC14 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546296
VERSION     XP_076546296.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690181.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..732
                     /product="protein VAC14 homolog isoform X2"
                     /calculated_mol_wt=82531
     Region          53..78
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          67..163
                     /region_name="Vac14_Fab1_bd"
                     /note="Vacuolar 14 Fab1-binding region; pfam12755"
                     /db_xref="CDD:403838"
     Site            order(70..71,74,77..78,111..112,115,118..119,122,152..153,
                     155,158..159,162,193..194,197,200..201,234..235,238,
                     241..242)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          94..122
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          134..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          172..201
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          214..243
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          499..675
                     /region_name="Vac14_Fig4_bd"
                     /note="Vacuolar protein 14 C-terminal Fig4p binding;
                     pfam11916"
                     /db_xref="CDD:463395"
     CDS             1..732
                     /gene="LOC117604760"
                     /coded_by="XM_076690181.1:121..2319"
                     /db_xref="GeneID:117604760"
ORIGIN      
        1 mmserdyapl stacvrslnd klyekrkpaa leiekmvkef sahnntvqik rllkvlgqdf
       61 atsqnphtrk ggliglaaia vglgkdtgqy iedlihpila cfcdsdlrvr yyaceslynv
      121 vkvargavlp qftdifaals klacdseqnv knatelldrl mkdivtesgl fdlvgfmpll
      181 reriytknaf grqfviawvs vldavpnmdf iiflpeildg lfkiledptp eikkvtdtvl
      241 geflrsikan pgrvdfpgmi nilithaqst dellqltait wikefvqlsg plmlpymsgi
      301 liavlpclay dgdtrkcike tatqvnanlm klvtmentqv vnnasendvq tqkgnvilvs
      361 ivkrkiavsi tflnflslds sqnrslaesl dlssvvevlt khlmymsvqt kvavlkwihh
      421 lfiniphkmf nhienlfpil mkslsdnsde vvqqtlvvma eiisskspka attdsnaemq
      481 nkyftkfivn llrlfstdrh lleergafii relcvllsae diyktlakil ldepnlsfac
      541 tmiqtlsvil ltsselfdlr nklkdldsle scklfkclyv swchnpvatv alcflsqiyg
      601 haydiirsfe nievtveflt eidklvqlie spiftylrlq llqredalvy alygllmilp
      661 qseayatlqr rlaaippttk pipksesssk sfkdrldfpe llkhfhtiqe qhkeqklfsf
      721 pgslstkckk ls
//
LOCUS       XP_034181084             707 aa            linear   INV 03-SEP-2025
DEFINITION  protein VAC14 homolog isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034181084
VERSION     XP_034181084.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..707
                     /product="protein VAC14 homolog isoform X3"
                     /calculated_mol_wt=79973
     Region          53..78
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          67..163
                     /region_name="Vac14_Fab1_bd"
                     /note="Vacuolar 14 Fab1-binding region; pfam12755"
                     /db_xref="CDD:403838"
     Site            order(70..71,74,77..78,111..112,115,118..119,122,152..153,
                     155,158..159,162,193..194,197,200..201,234..235,238,
                     241..242)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          94..122
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          134..163
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          172..201
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          214..243
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          474..650
                     /region_name="Vac14_Fig4_bd"
                     /note="Vacuolar protein 14 C-terminal Fig4p binding;
                     pfam11916"
                     /db_xref="CDD:463395"
     CDS             1..707
                     /gene="LOC117604760"
                     /coded_by="XM_034325193.2:119..2242"
                     /db_xref="GeneID:117604760"
ORIGIN      
        1 mmserdyapl stacvrslnd klyekrkpaa leiekmvkef sahnntvqik rllkvlgqdf
       61 atsqnphtrk ggliglaaia vglgkdtgqy iedlihpila cfcdsdlrvr yyaceslynv
      121 vkvargavlp qftdifaals klacdseqnv knatelldrl mkdivtesgl fdlvgfmpll
      181 reriytknaf grqfviawvs vldavpnmdf iiflpeildg lfkiledptp eikkvtdtvl
      241 geflrsikan pgrvdfpgmi nilithaqst dellqltait wikefvqlsg plmlpymsgi
      301 liavlpclay dgdtrkcike tatqvnanlm klvtmentqv vnnasendvq tqkdssqnrs
      361 laesldlssv vevltkhlmy msvqtkvavl kwihhlfini phkmfnhien lfpilmksls
      421 dnsdevvqqt lvvmaeiiss kspkaattds naemqnkyft kfivnllrlf stdrhlleer
      481 gafiirelcv llsaediykt lakilldepn lsfactmiqt lsvilltsse lfdlrnklkd
      541 ldslescklf kclyvswchn pvatvalcfl sqiyghaydi irsfenievt veflteidkl
      601 vqliespift ylrlqllqre dalvyalygl lmilpqseay atlqrrlaai ppttkpipks
      661 esssksfkdr ldfpellkhf htiqeqhkeq krkqrltnli eknngdi
//
LOCUS       XP_034181107             512 aa            linear   INV 03-SEP-2025
DEFINITION  rab11 family-interacting protein nuf isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181107
VERSION     XP_034181107.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325216.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181107.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="rab11 family-interacting protein nuf isoform X1"
                     /calculated_mol_wt=56586
     Region          <217..>414
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          469..509
                     /region_name="RBD-FIP"
                     /note="FIP domain; pfam09457"
                     /db_xref="CDD:462805"
     CDS             1..512
                     /gene="nuf"
                     /coded_by="XM_034325216.2:270..1808"
                     /db_xref="GeneID:117604770"
ORIGIN      
        1 mvssrstgvs vspansvats rvvvsdsdva rdgdailpsg rqsyraeneg grvngspagv
       61 ewsesgppsl smsvelhnlv npnlqdsehy saspehtdaa asscnvgtvr nndeeesyea
      121 fgsvegdadd gvdspggsss apsptgtnsl rnprspnsti grhswlrtsl rrtppcsgrk
      181 rlssnalasq lyrsgsfnss grgsncdptd dmysdvsled dvidlnhrvq miqeqmhalv
      241 dtqsvgeery arakqenatl qarilmlees akdaearaee rlqaeqrrhr ewasrlerer
      301 qlqlenyaik lqaielegtn lrdeivrlre qvdklktekg rlendledar reidtarese
      361 rqaisranea hyqleaaree lsmkiedqqr ieelmqqvaq lrahnkslee ardelqaaaa
      421 lqagrellml npcnnatekg pslavellag mnqdqmdgqi pgdndepcsi semrqalkeq
      481 qevntqlray idgillnive nypqllevkq sh
//
LOCUS       XP_034181117             512 aa            linear   INV 03-SEP-2025
DEFINITION  rab11 family-interacting protein nuf isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181117
VERSION     XP_034181117.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325226.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181117.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="rab11 family-interacting protein nuf isoform X1"
                     /calculated_mol_wt=56586
     Region          <217..>414
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          469..509
                     /region_name="RBD-FIP"
                     /note="FIP domain; pfam09457"
                     /db_xref="CDD:462805"
     CDS             1..512
                     /gene="nuf"
                     /coded_by="XM_034325226.2:265..1803"
                     /db_xref="GeneID:117604770"
ORIGIN      
        1 mvssrstgvs vspansvats rvvvsdsdva rdgdailpsg rqsyraeneg grvngspagv
       61 ewsesgppsl smsvelhnlv npnlqdsehy saspehtdaa asscnvgtvr nndeeesyea
      121 fgsvegdadd gvdspggsss apsptgtnsl rnprspnsti grhswlrtsl rrtppcsgrk
      181 rlssnalasq lyrsgsfnss grgsncdptd dmysdvsled dvidlnhrvq miqeqmhalv
      241 dtqsvgeery arakqenatl qarilmlees akdaearaee rlqaeqrrhr ewasrlerer
      301 qlqlenyaik lqaielegtn lrdeivrlre qvdklktekg rlendledar reidtarese
      361 rqaisranea hyqleaaree lsmkiedqqr ieelmqqvaq lrahnkslee ardelqaaaa
      421 lqagrellml npcnnatekg pslavellag mnqdqmdgqi pgdndepcsi semrqalkeq
      481 qevntqlray idgillnive nypqllevkq sh
//
LOCUS       XP_034181127             499 aa            linear   INV 03-SEP-2025
DEFINITION  rab11 family-interacting protein nuf isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181127
VERSION     XP_034181127.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325236.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181127.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..499
                     /product="rab11 family-interacting protein nuf isoform X2"
                     /calculated_mol_wt=55258
     Region          <204..>401
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          456..496
                     /region_name="RBD-FIP"
                     /note="FIP domain; pfam09457"
                     /db_xref="CDD:462805"
     CDS             1..499
                     /gene="nuf"
                     /coded_by="XM_034325236.2:270..1769"
                     /db_xref="GeneID:117604770"
ORIGIN      
        1 mvssrstgvs vspansvats rvvvsdsdva rdgdailpsg rqsyraeneg grvngspagv
       61 ewsesgppsl smsvelhnlv npnlqdsehy saspehtdaa asscnvgtvr nndeeesyea
      121 fgsvegdadd gvdspggsss apsptgtnsl rnprspnsti grhswlrtsl rrtppcqlyr
      181 sgsfnssgrg sncdptddmy sdvsleddvi dlnhrvqmiq eqmhalvdtq svgeeryara
      241 kqenatlqar ilmleesakd aearaeerlq aeqrrhrewa srlererqlq lenyaiklqa
      301 ielegtnlrd eivrlreqvd klktekgrle ndledarrei dtareserqa israneahyq
      361 leaareelsm kiedqqriee lmqqvaqlra hnksleeard elqaaaalqa grellmlnpc
      421 nnatekgpsl avellagmnq dqmdgqipgd ndepcsisem rqalkeqqev ntqlrayidg
      481 illnivenyp qllevkqsh
//
LOCUS       XP_034181139             287 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604782 [Osmia lignaria lignaria].
ACCESSION   XP_034181139
VERSION     XP_034181139.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325248.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="uncharacterized protein LOC117604782"
                     /calculated_mol_wt=33076
     Region          <138..178
                     /region_name="Nob1"
                     /note="rRNA maturation endonuclease Nob1 [Translation,
                     ribosomal structure and biogenesis]; COG1439"
                     /db_xref="CDD:441048"
     Region          141..161
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          168..188
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(173,175,177,179..180,183..184,187,201,203,207..208,
                     211..212,215,229,231,233,235..236,239..240,243)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          189..>245
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          196..216
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          224..244
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..287
                     /gene="LOC117604782"
                     /coded_by="XM_034325248.2:438..1301"
                     /db_xref="GeneID:117604782"
ORIGIN      
        1 maydprnvky ssemhshqhd qpnmtgytys ghsaqhvqqt denrnennyg nketctqish
       61 qssgtqtiqk vdaatmtdpl qidfrltsey larcsstlgl pyvskyeeni tnsthkyqei
      121 rpkiefevep qhinglliyq cpecayrfed qealhehved hrqrphicdi cgaslkrkeh
      181 ldrhkqghnk nrpyqcnmcc kafkrnehla rhmiihsgsk nqvctecgka fyrkdhlkkh
      241 lqshnssrsk iqsssqstsq nngeeglsnf amimrqtgpp pfsilrt
//
LOCUS       XP_034181152             273 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604790 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181152
VERSION     XP_034181152.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325261.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="uncharacterized protein LOC117604790 isoform X1"
                     /calculated_mol_wt=31931
     Region          12..96
                     /region_name="MADF_DNA_bdg"
                     /note="Alcohol dehydrogenase transcription factor
                     Myb/SANT-like; pfam10545"
                     /db_xref="CDD:463144"
     Region          226..260
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..273
                     /gene="LOC117604790"
                     /coded_by="XM_034325261.2:1..822"
                     /db_xref="GeneID:117604790"
ORIGIN      
        1 mrdevkmdan rliaevykrp alwnqrhisy hnrevtnrvw meiasifklp kpvlkakwkg
       61 lrdtfraelk kdqvyrkskf hrnrpvwihf kslqflkeqm lprppiwers npcqdddrvp
      121 segegdglml kgssvtnrns ddrntianhl lsinsdnglk idnvnhidvk qepeenfdnl
      181 efqsvsdnlt enemmlqnis peqvligdsd tniglpvdpl egnrfddnyy flmsllphir
      241 alpadrrmll rmqmqelvyk evykknsddv atp
//
LOCUS       XP_034181161             241 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604790 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181161
VERSION     XP_034181161.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325270.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="uncharacterized protein LOC117604790 isoform X2"
                     /calculated_mol_wt=28170
     Region          <22..66
                     /region_name="GT1"
                     /note="myb-like, SANT family; cl23759"
                     /db_xref="CDD:474047"
     Region          194..228
                     /region_name="BESS"
                     /note="BESS motif; pfam02944"
                     /db_xref="CDD:460758"
     CDS             1..241
                     /gene="LOC117604790"
                     /coded_by="XM_034325270.2:120..845"
                     /db_xref="GeneID:117604790"
ORIGIN      
        1 miflefymkk vyihfknlep vlkakwkglr dtfraelkkd qvyrkskfhr nrpvwihfks
       61 lqflkeqmlp rppiwersnp cqdddrvpse gegdglmlkg ssvtnrnsdd rntianhlls
      121 insdnglkid nvnhidvkqe peenfdnlef qsvsdnlten emmlqnispe qvligdsdtn
      181 iglpvdpleg nrfddnyyfl msllphiral padrrmllrm qmqelvykev ykknsddvat
      241 p
//
LOCUS       XP_034181173             243 aa            linear   INV 03-SEP-2025
DEFINITION  TP53 regulating kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181173
VERSION     XP_034181173.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325282.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="TP53 regulating kinase isoform X1"
                     /calculated_mol_wt=27757
     Region          16..237
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(17..20,23,25,36,38,67,91..94,141,145..146,148,
                     169..170)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..243
                     /gene="Prpk"
                     /coded_by="XM_034325282.2:143..874"
                     /db_xref="GeneID:117604800"
ORIGIN      
        1 mllkkqmlnn mkgfeliaqg aearvykgiy lgkptliker fekkyrhadl dirltkdrik
       61 aecraivrak aagvatpaiy lvslerrsiy meyidnavil kdfiyknisk ksdvdnllnf
      121 vtqglgmvia qlhskniihg dlttsnvlvk nvedinvenc dvvnnfvmid fglarvessv
      181 edkavdlyvl erslnsahse ipelfpkiyk tyqkyytnkn qckeiiskyn evqargrkrl
      241 mig
//
LOCUS       XP_034181182             237 aa            linear   INV 03-SEP-2025
DEFINITION  TP53 regulating kinase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181182
VERSION     XP_034181182.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325291.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="TP53 regulating kinase isoform X2"
                     /calculated_mol_wt=27015
     Region          10..231
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(11..14,17,19,30,32,61,85..88,135,139..140,142,
                     163..164)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..237
                     /gene="Prpk"
                     /coded_by="XM_034325291.2:98..811"
                     /db_xref="GeneID:117604800"
ORIGIN      
        1 mlnnmkgfel iaqgaearvy kgiylgkptl ikerfekkyr hadldirltk drikaecrai
       61 vrakaagvat paiylvsler rsiymeyidn avilkdfiyk niskksdvdn llnfvtqglg
      121 mviaqlhskn iihgdlttsn vlvknvedin vencdvvnnf vmidfglarv essvedkavd
      181 lyvlerslns ahseipelfp kiyktyqkyy tnknqckeii skynevqarg rkrlmig
//
LOCUS       XP_034181192             128 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604813 [Osmia lignaria lignaria].
ACCESSION   XP_034181192
VERSION     XP_034181192.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325301.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..128
                     /product="uncharacterized protein LOC117604813"
                     /calculated_mol_wt=14354
     CDS             1..128
                     /gene="LOC117604813"
                     /coded_by="XM_034325301.2:285..671"
                     /db_xref="GeneID:117604813"
ORIGIN      
        1 mcaanspcrs pprsgspskg npdyrfpqqq ppqppppppy vyypppyhpy npyapyhpga
       61 fysspycndv tqeskglswi sifliiflll givsvvfyls rdtrrrinag lptliqpaqd
      121 ynrgervl
//
LOCUS       XP_034181206             124 aa            linear   INV 03-SEP-2025
DEFINITION  GEL complex subunit OPTI [Osmia lignaria lignaria].
ACCESSION   XP_034181206
VERSION     XP_034181206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325315.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="GEL complex subunit OPTI"
                     /calculated_mol_wt=13961
     Region          39..115
                     /region_name="EMC6"
                     /note="pfam07019"
                     /db_xref="CDD:462067"
     CDS             1..124
                     /gene="LOC117604821"
                     /coded_by="XM_034325315.2:367..741"
                     /db_xref="GeneID:117604821"
ORIGIN      
        1 msrskserig nggkcelsvw traitansew pdkeefldvi ywarqvigii vgiawglipl
       61 kgfialllfv lvnagityly fsnfqqidee efggvweltk egfmtsfagf lvtwiiiysg
      121 lhfd
//
LOCUS       XP_034181215             124 aa            linear   INV 03-SEP-2025
DEFINITION  GEL complex subunit OPTI [Osmia lignaria lignaria].
ACCESSION   XP_034181215
VERSION     XP_034181215.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325324.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="GEL complex subunit OPTI"
                     /calculated_mol_wt=13961
     Region          39..115
                     /region_name="EMC6"
                     /note="pfam07019"
                     /db_xref="CDD:462067"
     CDS             1..124
                     /gene="LOC117604821"
                     /coded_by="XM_034325324.2:305..679"
                     /db_xref="GeneID:117604821"
ORIGIN      
        1 msrskserig nggkcelsvw traitansew pdkeefldvi ywarqvigii vgiawglipl
       61 kgfialllfv lvnagityly fsnfqqidee efggvweltk egfmtsfagf lvtwiiiysg
      121 lhfd
//
LOCUS       XP_034181228             118 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604831 [Osmia lignaria lignaria].
ACCESSION   XP_034181228
VERSION     XP_034181228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325337.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..118
                     /product="uncharacterized protein LOC117604831"
                     /calculated_mol_wt=13406
     CDS             1..118
                     /gene="LOC117604831"
                     /coded_by="XM_034325337.2:226..582"
                     /db_xref="GeneID:117604831"
ORIGIN      
        1 mtrsnrkrgr daeeessefm plskrinnlh inslsgmqec msenmdtdcs grsfmpspny
       61 selsqgsply dgcsssqssy tteykpdlda senpfyyesn kllfslymer vqrtsdsf
//
LOCUS       XP_034181245             118 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604831 [Osmia lignaria lignaria].
ACCESSION   XP_034181245
VERSION     XP_034181245.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325354.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..118
                     /product="uncharacterized protein LOC117604831"
                     /calculated_mol_wt=13406
     CDS             1..118
                     /gene="LOC117604831"
                     /coded_by="XM_034325354.2:230..586"
                     /db_xref="GeneID:117604831"
ORIGIN      
        1 mtrsnrkrgr daeeessefm plskrinnlh inslsgmqec msenmdtdcs grsfmpspny
       61 selsqgsply dgcsssqssy tteykpdlda senpfyyesn kllfslymer vqrtsdsf
//
LOCUS       XP_034181235             118 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604831 [Osmia lignaria lignaria].
ACCESSION   XP_034181235
VERSION     XP_034181235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325344.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..118
                     /product="uncharacterized protein LOC117604831"
                     /calculated_mol_wt=13406
     CDS             1..118
                     /gene="LOC117604831"
                     /coded_by="XM_034325344.2:292..648"
                     /db_xref="GeneID:117604831"
ORIGIN      
        1 mtrsnrkrgr daeeessefm plskrinnlh inslsgmqec msenmdtdcs grsfmpspny
       61 selsqgsply dgcsssqssy tteykpdlda senpfyyesn kllfslymer vqrtsdsf
//
LOCUS       XP_076546297             339 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611020 [Osmia lignaria lignaria].
ACCESSION   XP_076546297
VERSION     XP_076546297.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690182.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 41% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="uncharacterized protein LOC117611020"
                     /calculated_mol_wt=38907
     CDS             1..339
                     /gene="LOC117611020"
                     /coded_by="XM_076690182.1:11..1030"
                     /db_xref="GeneID:117611020"
ORIGIN      
        1 meinnfrigk rmleekawns lvtwheerll fgkpttmprk kqelgtiafq gcegewkggk
       61 midvdtsgek eieedegker idelaeskes keskgsrgei reaiaatkaa lvrieqqekk
      121 tteliqsvnk lceissqals qcqesvrkie tvatnanemm sevrkelvlw reersaegqr
      181 srdlrqtvrq dyatqiqrvy emvnqlsdsy nkldkgekrk dhgersseqq yevrdprekd
      241 pkpnvgkhds lndvggsdfk keianavrse ienlgnikvs qfkdrenkdt lkleivnsel
      301 racgplhvis dkdnvggide nilreqrfka rdviinrhy
//
LOCUS       XP_034175967             720 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602273 [Osmia lignaria lignaria].
ACCESSION   XP_034175967
VERSION     XP_034175967.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320076.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..720
                     /product="uncharacterized protein LOC117602273"
                     /calculated_mol_wt=79526
     CDS             1..720
                     /gene="LOC117602273"
                     /coded_by="XM_034320076.2:35..2197"
                     /db_xref="GeneID:117602273"
ORIGIN      
        1 mrilliqitt vllisyakrt sgrhlpstvd vdgeeesvss qfdidddskt deskedpsed
       61 klpilpplil ldfgndtees nvtseekskr tvnsglgygl ennalqprry nyyfpggksg
      121 ttvsieesis pflpktiver vqapsqrnyl dgyqnsfvss tdrysnlqqt ksqsvfgqrt
      181 kpqkvagass fesyqnvatt akpsadsfpv qnsgyqnvgi stqsplafsp sdsvryvtps
      241 pgnfashhsg sfnyastisp fsmnengrty aeqrlrsnvd srgfpvsqtv dsstvaplsy
      301 dshfpagsql snrprytven gvryenkifw kypdgrvsdv ppatyveytr ssagqsgksq
      361 qphaiyeptt tessvlsqgp vqfptvpepa aqepnpfvsg eslssslpqq qvyrlgyqsl
      421 vsqrqninla rqrkpnsnaa asysfstssp srrakpgsns akvnyqayqq psrymvngpn
      481 peytdgytte atlnsttpls nlfsssgdrs prkytskvrn yldsvlaeed gskkqsfdnn
      541 dlnsysnlry sdllnynpsi seyirnpssi lnvrptfvqa gnslipviil rvdgaspiqt
      601 kssqninlka llqqylvqya ksiqelaqps tydlgtesig kgqtsgqgks pvldlirltq
      661 edarqmpgyp tdsyigrssy etsnleepkq lsngryssrq kvknvqildd prfpnyrvkn
//
LOCUS       XP_076546298             215 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610931 [Osmia lignaria lignaria].
ACCESSION   XP_076546298
VERSION     XP_076546298.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690183.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="uncharacterized protein LOC117610931"
                     /calculated_mol_wt=24460
     Region          173..>207
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..215
                     /gene="LOC117610931"
                     /coded_by="XM_076690183.1:233..880"
                     /db_xref="GeneID:117610931"
ORIGIN      
        1 mqdssqesle vpgktrsart spvpsvtepv rrspspheha trstatdqlp ielqlkvrtq
       61 rcrlgnaqgi ldslkddser fskeelddak aqideaikvs nkehahfevv wptsqanhpy
      121 ftddchyqmq qigtvakkii akrsvelpgq tiaqpgkvty tksklldiel prftgnytew
      181 psflelfnsv vcgkleleqv ekfyylkgff fffff
//
LOCUS       XP_076546299             306 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305680 [Osmia lignaria lignaria].
ACCESSION   XP_076546299
VERSION     XP_076546299.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690184.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 54% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..306
                     /product="uncharacterized protein LOC143305680"
                     /calculated_mol_wt=35273
     Region          122..237
                     /region_name="DDE_Tnp_4"
                     /note="DDE superfamily endonuclease; pfam13359"
                     /db_xref="CDD:463855"
     CDS             1..306
                     /gene="LOC143305680"
                     /coded_by="XM_076690184.1:1..921"
                     /db_xref="GeneID:143305680"
ORIGIN      
        1 mdsvnegvav vsvmlmkelr nedvaafrni frlspdqfdv llqkvhplie kkctnmrepl
       61 ssklkleitl rylcsgdsfs sliylfrvai lkeylqvpst fekweriqre hrwnfpnvig
      121 tldgkhinis lpissgsdyy nykgkysivl maliykgrfs dgeifwnssf ytaltensln
      181 ilengiivad dafsrarrvv enafgiltat frifendikl qpekvekiif aacslhnwlr
      241 tckdenywpq eivdredtef gqiisghwrq itrgltnvha drfahnparh aineyceyfn
      301 gvgavp
//
LOCUS       XP_034184471             809 aa            linear   INV 03-SEP-2025
DEFINITION  putative pyridoxal-dependent decarboxylase domain-containing
            protein 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184471
VERSION     XP_034184471.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328580.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..809
                     /product="putative pyridoxal-dependent decarboxylase
                     domain-containing protein 2 isoform X1"
                     /calculated_mol_wt=88239
     Region          <192..434
                     /region_name="GadA"
                     /note="Glutamate or tyrosine decarboxylase or a related
                     PLP-dependent protein [Amino acid transport and
                     metabolism]; COG0076"
                     /db_xref="CDD:439846"
     CDS             1..809
                     /gene="LOC117606277"
                     /coded_by="XM_034328580.2:48..2477"
                     /db_xref="GeneID:117606277"
ORIGIN      
        1 mrrycattta tmaeetdtnd kaamesiaqn ilevstgggs asnevsalef qasqvisrle
       61 eaveraadgg gttwnnpsgf lsqskttdei ltliqdlvih edapqpvedg stetapsqvt
      121 tlptltepak laliahtvsa yalalpksha qrlagrlaad ttrwlshifr fvdcaasfhe
      181 dhleglvrvt rlalhrkypr ymeegftala asppliyssv aaplgvvqhl crqlslplhc
      241 vrpiphntmf gsrysmdvsa lerrlaedtq snnvtpllvl aeagsvltgh cdniarlrai
      301 cdkynvwlhl rghslaalal nnsakdlpsk iadsitlplg vwlgipslpv vtlyrltdtk
      361 ggrptprdtt lslvsglmtd slsrrlptlp lwatlqvlgr dgvynrlkrc flaveelynk
      421 ikkfsciril sqppggetgs ytinelltnp vnnpqlfetv asalvfqfvp adrdpqaser
      481 vppyydklns wlgqilqrdi envqielcei ehhgvairic plenpeqpps sedidnvvac
      541 leqqieilla tvehkatfvr lvsendslhl vempgwaglg gvryapptwi hvmtdqakee
      601 lnrlntqlve alrntdaafs lgegadglac vrfgmvtqdt dvaellslvl qvgqeveass
      661 klldtisevv krgieaaqtd lerenaeklw qegilrhvpv vgtfvnwwsp psketgvrgr
      721 slnlqagvve steniykyhm qlqpnstvin gsgartppqp lvqtpvgags qshsrssshs
      781 slqsgksvsv itnavshpqv keessqvks
//
LOCUS       XP_034184476             806 aa            linear   INV 03-SEP-2025
DEFINITION  putative pyridoxal-dependent decarboxylase domain-containing
            protein 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184476
VERSION     XP_034184476.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328585.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..806
                     /product="putative pyridoxal-dependent decarboxylase
                     domain-containing protein 2 isoform X2"
                     /calculated_mol_wt=87927
     Region          <192..431
                     /region_name="GadA"
                     /note="Glutamate or tyrosine decarboxylase or a related
                     PLP-dependent protein [Amino acid transport and
                     metabolism]; COG0076"
                     /db_xref="CDD:439846"
     CDS             1..806
                     /gene="LOC117606277"
                     /coded_by="XM_034328585.2:48..2468"
                     /db_xref="GeneID:117606277"
ORIGIN      
        1 mrrycattta tmaeetdtnd kaamesiaqn ilevstgggs asnevsalef qasqvisrle
       61 eaveraadgg gttwnnpsgf lsqskttdei ltliqdlvih edapqpvedg stetapsqvt
      121 tlptltepak laliahtvsa yalalpksha qrlagrlaad ttrwlshifr fvdcaasfhe
      181 dhleglvrvt rlalhrkypr ymeegftala asppliyssv aaplgvvqhl crqlslplhc
      241 vrpiphntmf gsrysmdvsa lerrlaedtq snnvtpllvl aeagsvltgh cdniarlrai
      301 cdkynvwlhl rghslaalal nnsakdliad sitlplgvwl gipslpvvtl yrltdtkggr
      361 ptprdttlsl vsglmtdsls rrlptlplwa tlqvlgrdgv ynrlkrcfla veelynkikk
      421 fscirilsqp pggetgsyti nelltnpvnn pqlfetvasa lvfqfvpadr dpqaservpp
      481 yydklnswlg qilqrdienv qielceiehh gvairicple npeqppssed idnvvacleq
      541 qieillatve hkatfvrlvs endslhlvem pgwaglggvr yapptwihvm tdqakeelnr
      601 lntqlvealr ntdaafslge gadglacvrf gmvtqdtdva ellslvlqvg qeveasskll
      661 dtisevvkrg ieaaqtdler enaeklwqeg ilrhvpvvgt fvnwwsppsk etgvrgrsln
      721 lqagvveste niykyhmqlq pnstvingsg artppqplvq tpvgagsqsh srssshsslq
      781 sgksvsvitn avshpqvkee ssqvks
//
LOCUS       XP_034184492             564 aa            linear   INV 03-SEP-2025
DEFINITION  tweety isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184492
VERSION     XP_034184492.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184492.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="tweety isoform X1"
                     /calculated_mol_wt=61401
     Region          10..429
                     /region_name="Tweety_N"
                     /note="N-terminal domain of the protein encoded by the
                     Drosophila tweety gene and related proteins, a family of
                     chloride ion channels; cd07912"
                     /db_xref="CDD:143653"
     Site            order(375,378)
                     /site_type="active"
                     /note="putative pore region [active]"
                     /db_xref="CDD:143653"
     CDS             1..564
                     /gene="tty"
                     /coded_by="XM_034328601.2:215..1909"
                     /db_xref="GeneID:117606288"
ORIGIN      
        1 maggqqlpeq ytvpplarvf hslphlnvsl hsvnntfnph seiyleslgi lgsipaawli
       61 ltllvllvyl vtrccdrkpr pkrsitllkf slailallcc gavavglygn ddvhnglvql
      121 vaaaknvdgi lmgvrnqtdt ientlkkkve pqltalrdef ddpvsnktmi elllaalqsm
      181 kqntsiavnk siemrkpltg islagaigmg ekieqlrwpi tmavlsallv lcvvllvgva
      241 rhsrcvlitf svfglfaviv swlmaslyla tsvalgdlcv spdgyltrav pstlasevls
      301 yythcentrs npftqrlrdg qtaisemskn lntvtrlale lykdkqlqpk lnsllfdvnt
      361 vsklmsglat lldckpfhkq yvhaakslch lglygltfml laslaaglff tvlvwvdsht
      421 wiyirkrrdy hqvdeqdpyl ppsaasqaia artlrgqgsy pptapsvfyp nahgtqntik
      481 qpllltpppp syatatarar qlhesmlkgg gingsggggd hkssqhnqqs tggraehrsg
      541 lgdqpgqyat lskqcktles sdfy
//
LOCUS       XP_034184500             564 aa            linear   INV 03-SEP-2025
DEFINITION  tweety isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184500
VERSION     XP_034184500.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328609.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184500.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="tweety isoform X1"
                     /calculated_mol_wt=61401
     Region          10..429
                     /region_name="Tweety_N"
                     /note="N-terminal domain of the protein encoded by the
                     Drosophila tweety gene and related proteins, a family of
                     chloride ion channels; cd07912"
                     /db_xref="CDD:143653"
     Site            order(375,378)
                     /site_type="active"
                     /note="putative pore region [active]"
                     /db_xref="CDD:143653"
     CDS             1..564
                     /gene="tty"
                     /coded_by="XM_034328609.2:1325..3019"
                     /db_xref="GeneID:117606288"
ORIGIN      
        1 maggqqlpeq ytvpplarvf hslphlnvsl hsvnntfnph seiyleslgi lgsipaawli
       61 ltllvllvyl vtrccdrkpr pkrsitllkf slailallcc gavavglygn ddvhnglvql
      121 vaaaknvdgi lmgvrnqtdt ientlkkkve pqltalrdef ddpvsnktmi elllaalqsm
      181 kqntsiavnk siemrkpltg islagaigmg ekieqlrwpi tmavlsallv lcvvllvgva
      241 rhsrcvlitf svfglfaviv swlmaslyla tsvalgdlcv spdgyltrav pstlasevls
      301 yythcentrs npftqrlrdg qtaisemskn lntvtrlale lykdkqlqpk lnsllfdvnt
      361 vsklmsglat lldckpfhkq yvhaakslch lglygltfml laslaaglff tvlvwvdsht
      421 wiyirkrrdy hqvdeqdpyl ppsaasqaia artlrgqgsy pptapsvfyp nahgtqntik
      481 qpllltpppp syatatarar qlhesmlkgg gingsggggd hkssqhnqqs tggraehrsg
      541 lgdqpgqyat lskqcktles sdfy
//
LOCUS       XP_034184507             564 aa            linear   INV 03-SEP-2025
DEFINITION  tweety isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184507
VERSION     XP_034184507.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328616.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184507.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="tweety isoform X1"
                     /calculated_mol_wt=61401
     Region          10..429
                     /region_name="Tweety_N"
                     /note="N-terminal domain of the protein encoded by the
                     Drosophila tweety gene and related proteins, a family of
                     chloride ion channels; cd07912"
                     /db_xref="CDD:143653"
     Site            order(375,378)
                     /site_type="active"
                     /note="putative pore region [active]"
                     /db_xref="CDD:143653"
     CDS             1..564
                     /gene="tty"
                     /coded_by="XM_034328616.2:392..2086"
                     /db_xref="GeneID:117606288"
ORIGIN      
        1 maggqqlpeq ytvpplarvf hslphlnvsl hsvnntfnph seiyleslgi lgsipaawli
       61 ltllvllvyl vtrccdrkpr pkrsitllkf slailallcc gavavglygn ddvhnglvql
      121 vaaaknvdgi lmgvrnqtdt ientlkkkve pqltalrdef ddpvsnktmi elllaalqsm
      181 kqntsiavnk siemrkpltg islagaigmg ekieqlrwpi tmavlsallv lcvvllvgva
      241 rhsrcvlitf svfglfaviv swlmaslyla tsvalgdlcv spdgyltrav pstlasevls
      301 yythcentrs npftqrlrdg qtaisemskn lntvtrlale lykdkqlqpk lnsllfdvnt
      361 vsklmsglat lldckpfhkq yvhaakslch lglygltfml laslaaglff tvlvwvdsht
      421 wiyirkrrdy hqvdeqdpyl ppsaasqaia artlrgqgsy pptapsvfyp nahgtqntik
      481 qpllltpppp syatatarar qlhesmlkgg gingsggggd hkssqhnqqs tggraehrsg
      541 lgdqpgqyat lskqcktles sdfy
//
LOCUS       XP_076546300             563 aa            linear   INV 03-SEP-2025
DEFINITION  tweety isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546300
VERSION     XP_076546300.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690185.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..563
                     /product="tweety isoform X2"
                     /calculated_mol_wt=61245
     Region          10..428
                     /region_name="Tweety_N"
                     /note="N-terminal domain of the protein encoded by the
                     Drosophila tweety gene and related proteins, a family of
                     chloride ion channels; cd07912"
                     /db_xref="CDD:143653"
     Site            order(375,378)
                     /site_type="active"
                     /note="putative pore region [active]"
                     /db_xref="CDD:143653"
     CDS             1..563
                     /gene="tty"
                     /coded_by="XM_076690185.1:215..1906"
                     /db_xref="GeneID:117606288"
ORIGIN      
        1 maggqqlpeq ytvpplarvf hslphlnvsl hsvnntfnph seiyleslgi lgsipaawli
       61 ltllvllvyl vtrccdrkpr pkrsitllkf slailallcc gavavglygn ddvhnglvql
      121 vaaaknvdgi lmgvrnqtdt ientlkkkve pqltalrdef ddpvsnktmi elllaalqsm
      181 kqntsiavnk siemrkpltg islagaigmg ekieqlrwpi tmavlsallv lcvvllvgva
      241 rhsrcvlitf svfglfaviv swlmaslyla tsvalgdlcv spdgyltrav pstlasevls
      301 yythcentrs npftqrlrdg qtaisemskn lntvtrlale lykdkqlqpk lnsllfdvnt
      361 vsklmsglat lldckpfhkq yvhaakslch lglygltfml laslaaglff tvlvwvdsht
      421 wiyirkrdyh qvdeqdpylp psaasqaiaa rtlrgqgsyp ptapsvfypn ahgtqntikq
      481 pllltpppps yatatararq lhesmlkggg ingsggggdh kssqhnqqst ggraehrsgl
      541 gdqpgqyatl skqcktless dfy
//
LOCUS       XP_076546301             514 aa            linear   INV 03-SEP-2025
DEFINITION  tweety isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546301
VERSION     XP_076546301.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690186.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="tweety isoform X3"
                     /calculated_mol_wt=55958
     Region          10..429
                     /region_name="Tweety_N"
                     /note="N-terminal domain of the protein encoded by the
                     Drosophila tweety gene and related proteins, a family of
                     chloride ion channels; cd07912"
                     /db_xref="CDD:143653"
     Site            order(375,378)
                     /site_type="active"
                     /note="putative pore region [active]"
                     /db_xref="CDD:143653"
     CDS             1..514
                     /gene="tty"
                     /coded_by="XM_076690186.1:215..1759"
                     /db_xref="GeneID:117606288"
ORIGIN      
        1 maggqqlpeq ytvpplarvf hslphlnvsl hsvnntfnph seiyleslgi lgsipaawli
       61 ltllvllvyl vtrccdrkpr pkrsitllkf slailallcc gavavglygn ddvhnglvql
      121 vaaaknvdgi lmgvrnqtdt ientlkkkve pqltalrdef ddpvsnktmi elllaalqsm
      181 kqntsiavnk siemrkpltg islagaigmg ekieqlrwpi tmavlsallv lcvvllvgva
      241 rhsrcvlitf svfglfaviv swlmaslyla tsvalgdlcv spdgyltrav pstlasevls
      301 yythcentrs npftqrlrdg qtaisemskn lntvtrlale lykdkqlqpk lnsllfdvnt
      361 vsklmsglat lldckpfhkq yvhaakslch lglygltfml laslaaglff tvlvwvdsht
      421 wiyirkrrdy hqvdeqdpyl ppsaasqaia artlrgqggg gingsggggd hkssqhnqqs
      481 tggraehrsg lgdqpgqyat lskqcktles sdfy
//
LOCUS       XP_076546302             513 aa            linear   INV 03-SEP-2025
DEFINITION  tweety isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546302
VERSION     XP_076546302.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690187.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..513
                     /product="tweety isoform X4"
                     /calculated_mol_wt=55802
     Region          10..428
                     /region_name="Tweety_N"
                     /note="N-terminal domain of the protein encoded by the
                     Drosophila tweety gene and related proteins, a family of
                     chloride ion channels; cd07912"
                     /db_xref="CDD:143653"
     Site            order(375,378)
                     /site_type="active"
                     /note="putative pore region [active]"
                     /db_xref="CDD:143653"
     CDS             1..513
                     /gene="tty"
                     /coded_by="XM_076690187.1:215..1756"
                     /db_xref="GeneID:117606288"
ORIGIN      
        1 maggqqlpeq ytvpplarvf hslphlnvsl hsvnntfnph seiyleslgi lgsipaawli
       61 ltllvllvyl vtrccdrkpr pkrsitllkf slailallcc gavavglygn ddvhnglvql
      121 vaaaknvdgi lmgvrnqtdt ientlkkkve pqltalrdef ddpvsnktmi elllaalqsm
      181 kqntsiavnk siemrkpltg islagaigmg ekieqlrwpi tmavlsallv lcvvllvgva
      241 rhsrcvlitf svfglfaviv swlmaslyla tsvalgdlcv spdgyltrav pstlasevls
      301 yythcentrs npftqrlrdg qtaisemskn lntvtrlale lykdkqlqpk lnsllfdvnt
      361 vsklmsglat lldckpfhkq yvhaakslch lglygltfml laslaaglff tvlvwvdsht
      421 wiyirkrdyh qvdeqdpylp psaasqaiaa rtlrgqgggg ingsggggdh kssqhnqqst
      481 ggraehrsgl gdqpgqyatl skqcktless dfy
//
LOCUS       XP_034184535             434 aa            linear   INV 03-SEP-2025
DEFINITION  cytohesin steppke isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184535
VERSION     XP_034184535.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328644.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..434
                     /product="cytohesin steppke isoform X1"
                     /calculated_mol_wt=49718
     Region          100..280
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          295..412
                     /region_name="PH_GRP1-like"
                     /note="General Receptor for Phosphoinositides-1-like
                     Pleckstrin homology (PH) domain; cd01252"
                     /db_xref="CDD:269954"
     Site            order(295,322,324,326,336,339,341,370..372,374,377..382,
                     412)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:269954"
     Site            order(305,308,312,314,316,327,337,373,384..385)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:269954"
     CDS             1..434
                     /gene="step"
                     /coded_by="XM_034328644.2:669..1973"
                     /db_xref="GeneID:117606310"
ORIGIN      
        1 mmiftgrsvd aldevhgsnv qtdnrrdvsa iaiaagwfss lrrpgkrnkk gyqkqaksaw
       61 dlstlstqlk delgevvaem eameggglaa detkpsnkak qtsigrkkfn mdpkkgieyl
      121 iehnllaptp edvaqflykg eglnktaigd ylgerhdfne rvlrafvelh dftdlilvqa
      181 lrqflwsfrl pgeaqkidrm mecfaqrycq lnpniftntd tcyvlsfaii mlntslhnps
      241 vkdkpsveqf ismnrginng gdlprellvs lyesiktepf kipeddgndl mhtffnpdke
      301 gwlwkqggry kswkrrwfil ndnclyyfey ttdkeprgii pleniqvrev qdrhkphcfe
      361 lyaagsefik acktdsegkv vegkhtvyrm saatdeekee wikcvrqsis hnpfydmlaa
      421 rkkkaqktnv hsks
//
LOCUS       XP_076546303             418 aa            linear   INV 03-SEP-2025
DEFINITION  cytohesin steppke isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546303
VERSION     XP_076546303.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690188.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..418
                     /product="cytohesin steppke isoform X2"
                     /calculated_mol_wt=48014
     Region          84..264
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          279..396
                     /region_name="PH_GRP1-like"
                     /note="General Receptor for Phosphoinositides-1-like
                     Pleckstrin homology (PH) domain; cd01252"
                     /db_xref="CDD:269954"
     Site            order(279,306,308,310,320,323,325,354..356,358,361..366,
                     396)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:269954"
     Site            order(289,292,296,298,300,311,321,357,368..369)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:269954"
     CDS             1..418
                     /gene="step"
                     /coded_by="XM_076690188.1:81..1337"
                     /db_xref="GeneID:117606310"
ORIGIN      
        1 mwqdldglgg agvnssahqv dqfgtteltp eqqkilidir rkktelllei qqlkdelgev
       61 vaemeamegg glaadetkps nkakqtsigr kkfnmdpkkg ieyliehnll aptpedvaqf
      121 lykgeglnkt aigdylgerh dfnervlraf velhdftdli lvqalrqflw sfrlpgeaqk
      181 idrmmecfaq rycqlnpnif tntdtcyvls faiimlntsl hnpsvkdkps veqfismnrg
      241 innggdlpre llvslyesik tepfkipedd gndlmhtffn pdkegwlwkq ggrykswkrr
      301 wfilndncly yfeyttdkep rgiipleniq vrevqdrhkp hcfelyaags efikacktds
      361 egkvvegkht vyrmsaatde ekeewikcvr qsishnpfyd mlaarkkkaq ktnvhsks
//
LOCUS       XP_076546304             401 aa            linear   INV 03-SEP-2025
DEFINITION  cytohesin steppke isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546304
VERSION     XP_076546304.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690189.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..401
                     /product="cytohesin steppke isoform X3"
                     /calculated_mol_wt=46387
     Region          67..247
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          262..379
                     /region_name="PH_GRP1-like"
                     /note="General Receptor for Phosphoinositides-1-like
                     Pleckstrin homology (PH) domain; cd01252"
                     /db_xref="CDD:269954"
     Site            order(262,289,291,293,303,306,308,337..339,341,344..349,
                     379)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:269954"
     Site            order(272,275,279,281,283,294,304,340,351..352)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:269954"
     CDS             1..401
                     /gene="step"
                     /coded_by="XM_076690189.1:37..1242"
                     /db_xref="GeneID:117606310"
ORIGIN      
        1 mtsscrrsgr rgwfdscclc hvicffrgrr rarkqlkdel gevvaemeam eggglaadet
       61 kpsnkakqts igrkkfnmdp kkgieylieh nllaptpedv aqflykgegl nktaigdylg
      121 erhdfnervl rafvelhdft dlilvqalrq flwsfrlpge aqkidrmmec faqrycqlnp
      181 niftntdtcy vlsfaiimln tslhnpsvkd kpsveqfism nrginnggdl prellvslye
      241 siktepfkip eddgndlmht ffnpdkegwl wkqggryksw krrwfilndn clyyfeyttd
      301 keprgiiple niqvrevqdr hkphcfelya agsefikack tdsegkvveg khtvyrmsaa
      361 tdeekeewik cvrqsishnp fydmlaarkk kaqktnvhsk s
//
LOCUS       XP_076546305             368 aa            linear   INV 03-SEP-2025
DEFINITION  cytohesin steppke isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546305
VERSION     XP_076546305.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690190.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..368
                     /product="cytohesin steppke isoform X4"
                     /calculated_mol_wt=42441
     Region          34..214
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          229..346
                     /region_name="PH_GRP1-like"
                     /note="General Receptor for Phosphoinositides-1-like
                     Pleckstrin homology (PH) domain; cd01252"
                     /db_xref="CDD:269954"
     Site            order(229,256,258,260,270,273,275,304..306,308,311..316,
                     346)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:269954"
     Site            order(239,242,246,248,250,261,271,307,318..319)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:269954"
     CDS             1..368
                     /gene="step"
                     /coded_by="XM_076690190.1:869..1975"
                     /db_xref="GeneID:117606310"
ORIGIN      
        1 mqlkdelgev vaemeamegg glaadetkps nkakqtsigr kkfnmdpkkg ieyliehnll
       61 aptpedvaqf lykgeglnkt aigdylgerh dfnervlraf velhdftdli lvqalrqflw
      121 sfrlpgeaqk idrmmecfaq rycqlnpnif tntdtcyvls faiimlntsl hnpsvkdkps
      181 veqfismnrg innggdlpre llvslyesik tepfkipedd gndlmhtffn pdkegwlwkq
      241 ggrykswkrr wfilndncly yfeyttdkep rgiipleniq vrevqdrhkp hcfelyaags
      301 efikacktds egkvvegkht vyrmsaatde ekeewikcvr qsishnpfyd mlaarkkkaq
      361 ktnvhsks
//
LOCUS       XP_076546306             355 aa            linear   INV 03-SEP-2025
DEFINITION  cytohesin steppke isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546306
VERSION     XP_076546306.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690191.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="cytohesin steppke isoform X5"
                     /calculated_mol_wt=40998
     Region          21..201
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          216..333
                     /region_name="PH_GRP1-like"
                     /note="General Receptor for Phosphoinositides-1-like
                     Pleckstrin homology (PH) domain; cd01252"
                     /db_xref="CDD:269954"
     Site            order(216,243,245,247,257,260,262,291..293,295,298..303,
                     333)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:269954"
     Site            order(226,229,233,235,237,248,258,294,305..306)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:269954"
     CDS             1..355
                     /gene="step"
                     /coded_by="XM_076690191.1:268..1335"
                     /db_xref="GeneID:117606310"
ORIGIN      
        1 meamegggla adetkpsnka kqtsigrkkf nmdpkkgiey liehnllapt pedvaqflyk
       61 geglnktaig dylgerhdfn ervlrafvel hdftdlilvq alrqflwsfr lpgeaqkidr
      121 mmecfaqryc qlnpniftnt dtcyvlsfai imlntslhnp svkdkpsveq fismnrginn
      181 ggdlprellv slyesiktep fkipeddgnd lmhtffnpdk egwlwkqggr ykswkrrwfi
      241 lndnclyyfe yttdkeprgi ipleniqvre vqdrhkphcf elyaagsefi kacktdsegk
      301 vvegkhtvyr msaatdeeke ewikcvrqsi shnpfydmla arkkkaqktn vhsks
//
LOCUS       XP_076546307             355 aa            linear   INV 03-SEP-2025
DEFINITION  cytohesin steppke isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546307
VERSION     XP_076546307.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690192.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="cytohesin steppke isoform X5"
                     /calculated_mol_wt=40998
     Region          21..201
                     /region_name="Sec7"
                     /note="Sec7 domain; pfam01369"
                     /db_xref="CDD:460178"
     Region          216..333
                     /region_name="PH_GRP1-like"
                     /note="General Receptor for Phosphoinositides-1-like
                     Pleckstrin homology (PH) domain; cd01252"
                     /db_xref="CDD:269954"
     Site            order(216,243,245,247,257,260,262,291..293,295,298..303,
                     333)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:269954"
     Site            order(226,229,233,235,237,248,258,294,305..306)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:269954"
     CDS             1..355
                     /gene="step"
                     /coded_by="XM_076690192.1:138..1205"
                     /db_xref="GeneID:117606310"
ORIGIN      
        1 meamegggla adetkpsnka kqtsigrkkf nmdpkkgiey liehnllapt pedvaqflyk
       61 geglnktaig dylgerhdfn ervlrafvel hdftdlilvq alrqflwsfr lpgeaqkidr
      121 mmecfaqryc qlnpniftnt dtcyvlsfai imlntslhnp svkdkpsveq fismnrginn
      181 ggdlprellv slyesiktep fkipeddgnd lmhtffnpdk egwlwkqggr ykswkrrwfi
      241 lndnclyyfe yttdkeprgi ipleniqvre vqdrhkphcf elyaagsefi kacktdsegk
      301 vvegkhtvyr msaatdeeke ewikcvrqsi shnpfydmla arkkkaqktn vhsks
//
LOCUS       XP_034196401             352 aa            linear   INV 03-SEP-2025
DEFINITION  lactosylceramide 4-alpha-galactosyltransferase-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034196401
VERSION     XP_034196401.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340510.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="lactosylceramide
                     4-alpha-galactosyltransferase-like"
                     /calculated_mol_wt=39826
     Region          89..198
                     /region_name="Gly_transf_sug"
                     /note="Glycosyltransferase sugar-binding region containing
                     DXD motif; cl19952"
                     /db_xref="CDD:418730"
     Region          219..348
                     /region_name="Gb3_synth"
                     /note="Alpha 1,4-glycosyltransferase conserved region;
                     pfam04572"
                     /db_xref="CDD:428017"
     CDS             1..352
                     /gene="LOC117611962"
                     /coded_by="XM_034340510.2:706..1764"
                     /db_xref="GeneID:117611962"
ORIGIN      
        1 mkkrlllcfv cavilfilmi stdndfiqhv spfylsgsnd itcyegansa dnipdfnpks
       61 vskgstkdrn iffhetscfd dgglllnarq acavesaakm npktnvyllf vspakinnqs
      121 keffnqlrty pnihlghiyp eeyvkgtpld vwyksgvlkk srwprshmsd ilryltlwky
      181 ggiyldldvv vttslenltn fagaedwddv aagaigidat tlgrrvadac irdlmknfrg
      241 niwgnngpgv itrtlqrics akyvrdmtpa rcggfkvfpp stfypihykk wkmyfetknk
      301 natmkmieka maihvwnkls naqevrvdsd vpyaiiarkh cpkifnncgk mf
//
LOCUS       XP_034184592             184 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein L12 [Osmia lignaria lignaria].
ACCESSION   XP_034184592
VERSION     XP_034184592.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328701.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..184
                     /product="mitochondrial ribosomal protein L12"
                     /calculated_mol_wt=20414
     Region          54..183
                     /region_name="RplL"
                     /note="Ribosomal protein L7/L12 [Translation, ribosomal
                     structure and biogenesis]; COG0222"
                     /db_xref="CDD:439992"
     CDS             1..184
                     /gene="mRpL12"
                     /coded_by="XM_034328701.2:155..709"
                     /db_xref="GeneID:117606340"
ORIGIN      
        1 mmnavrfvcq rnvrqirqfh kcivqhteve attvstpaes syvstntdkk pevsskieqi
       61 asqivalnli evaqlsdllk krlnlpdapm mpmgafvgap kaeeeeeepq qvqtiftvkl
      121 kafkddqkva likemktilp dcnlvqakkf vesapvvvra dipkeeaekl keaiekvgge
      181 veiv
//
LOCUS       XP_076546308              91 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305681 [Osmia lignaria lignaria].
ACCESSION   XP_076546308
VERSION     XP_076546308.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690193.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..91
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..91
                     /product="uncharacterized protein LOC143305681"
                     /calculated_mol_wt=10418
     CDS             1..91
                     /gene="LOC143305681"
                     /coded_by="XM_076690193.1:1..276"
                     /db_xref="GeneID:143305681"
ORIGIN      
        1 mnqfrslvrn kapniriqdq mqdqvrtldr nftrnqfngt aqnnavpnmy gtynraqsrq
       61 drqdrqdnks tvatlsqvqg sdpnlyavte l
//
LOCUS       XP_076546309             755 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305682 [Osmia lignaria lignaria].
ACCESSION   XP_076546309
VERSION     XP_076546309.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690194.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 39% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..755
                     /product="uncharacterized protein LOC143305682"
                     /calculated_mol_wt=88007
     Region          <19..142
                     /region_name="transpos_IS481"
                     /note="IS481 family transposase; NF033577"
                     /db_xref="CDD:468094"
     Region          <358..>549
                     /region_name="Baculo_F"
                     /note="Baculovirus F protein; pfam12259"
                     /db_xref="CDD:371998"
     CDS             1..755
                     /gene="LOC143305682"
                     /coded_by="XM_076690194.1:1..2268"
                     /db_xref="GeneID:143305682"
ORIGIN      
        1 mdriiqnyin qcetclehky krrpyetety gpiianrplq hihmdifhfn kekfltiidi
       61 fskyaqayhl pdgnattiln klrhfsshhn fpdkittdsg sefnstvfke fcrihkidyh
      121 qttinrhtsn gpierlhstl keklsilidq npreitknhm ttailiynqs ihsstnyapf
      181 tllygpykel hkhiidpdsd tiekyneirk keilpfyekl yqkqlqkqhl pnnsdetles
      241 remfvkthqq qrdktapryq rrmkiiltlt isiilvksiq idpnathyny qqgigrimen
      301 yhylhfyini tslrhtynki lisytilsgr eelrnsslfr qtkslcneiy qnlqqiaplt
      361 ervkrgling lgtvikyifg npdandldki nnyltslerq qhediltlgk tvsiintisn
      421 tingntemin knlqnlantl seqstrldlf eaiitlitqe qhflnlleki krsfvfsgeg
      481 fdleiltyee msdikahllk lysrrelmlh yhdlldfrft qgsivtapds iiyilkipil
      541 npiefslfqr latishnnqt eiittpwklt gttvklfahk cqliydhdyl cyrivaeeir
      601 piyisinvpl vlayplsnns tliscnypit ieynrnkttl qgthfvqgnd vqvqghnlne
      661 nlgelkqsei lpliirnyql nleplktlts ptidiptfqk lgiefhmsvt svaiifliii
      721 gllvhftyrk kgkirqptqt innkdvielr qggvm
//
LOCUS       XP_076546310             721 aa            linear   INV 03-SEP-2025
DEFINITION  RUN and FYVE domain-containing protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546310
VERSION     XP_076546310.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690195.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..721
                     /product="RUN and FYVE domain-containing protein 2 isoform
                     X1"
                     /calculated_mol_wt=82921
     Region          135..290
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <339..424
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <410..>658
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          653..710
                     /region_name="FYVE_RUFY1_like"
                     /note="FYVE domain found in RUN and FYVE domain-containing
                     protein RUFY1, RUFY2 and similar proteins; cd15721"
                     /db_xref="CDD:277261"
     Site            order(653,656,674..679,681..682,704..706)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277261"
     CDS             1..721
                     /gene="LOC117609905"
                     /coded_by="XM_076690195.1:1071..3236"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsg inhhhhhhhh yhhhhhdhqr qhqlpnqrel rdiippsppp
      121 lqhvsrlsrd pviiersnlv nisklivkel ietslkygrm ldsdhmplqh ffivlehvlr
      181 hglrpkkgil gpkkelwdil qlvekycpea qditssirdl ptvrtamgra rawlrmalmq
      241 kkladylkvl idhkedilse yfepdalmms eeaivimgll vglnvidcnl cvkeedldcq
      301 qgvidfsiyl rnsnhipges pddelendnm ttvldqknyi eelnrhlnat vtnlqakves
      361 ltttnalmke dlaiaknnil slheenrqlk kelgievkdt nengkppiki tettaeieel
      421 rsrleaekkm rqdvekelel qismksemev amkllekdih ekqdtiislr rqldeiklin
      481 lemykklqec egslkhktel itkleaktls mtetiqkmde kckemdgvrs gaekrvrilg
      541 veaaerearv tgverelrle rewrtslqea sicnaekisq lhqeidqlrr vsekylalqe
      601 ehyalkeics eqertleelg gqlstaklva velreaadna qqqqqqqdga atwandrlvs
      661 hckscnrefn itrrkhhcrn cgkifcnacs dnttalstst kpvrvcdecy vflvgryssf
      721 t
//
LOCUS       XP_076546311             706 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546311
VERSION     XP_076546311.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690196.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..706
                     /product="protein RUFY3 isoform X2"
                     /calculated_mol_wt=80918
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..706
                     /gene="LOC117609905"
                     /coded_by="XM_076690196.1:1071..3191"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrktde lhtdgrttpe rrshqyrssl lpnptplftq
      661 llsrdetrtr ycsarrypig rnsfllsivf aifgkcsevs llsess
//
LOCUS       XP_076546312             690 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546312
VERSION     XP_076546312.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690197.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..690
                     /product="protein RUFY3 isoform X3"
                     /calculated_mol_wt=79125
     Region          71..226
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <275..360
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <346..>594
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          589..>611
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..690
                     /gene="LOC117609905"
                     /coded_by="XM_076690197.1:202..2274"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 maadsseglp ispseksltn slasdeneks asrspssfsi redkwpdlvv srprkldaww
       61 lprprdpvii ersnlvnisk livkeliets lkygrmldsd hmplqhffiv lehvlrhglr
      121 pkkgilgpkk elwdilqlve kycpeaqdit ssirdlptvr tamgrarawl rmalmqkkla
      181 dylkvlidhk edilseyfep dalmmseeai vimgllvgln vidcnlcvke edldcqqgvi
      241 dfsiylrnsn hipgespdde lendnmttvl dqknyieeln rhlnatvtnl qakveslttt
      301 nalmkedlai aknnilslhe enrqlkkelg ievkdtneng kppikitett aeieelrsrl
      361 eaekkmrqdv ekelelqism ksemevamkl lekdihekqd tiislrrqld eiklinlemy
      421 kklqecegsl khktelitkl eaktlsmtet iqkmdekcke mdgvrsgaek rvrilgveaa
      481 erearvtgve relrlerewr tslqeasicn aekisqlhqe idqlrrvsek ylalqeehya
      541 lkeicseqer tleelggqls taklvavelr eaadnaqqqq qqqdgaatwa ndrlvshcks
      601 cnrefnitrr ktdelhtdgr ttperrshqy rssllpnptp lftqllsrde trtrycsarr
      661 ypigrnsfll sivfaifgkc sevsllsess
//
LOCUS       XP_076546313             688 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546313
VERSION     XP_076546313.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690198.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..688
                     /product="protein RUFY3 isoform X4"
                     /calculated_mol_wt=79095
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..688
                     /gene="LOC117609905"
                     /coded_by="XM_076690198.1:1070..3136"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrksrs lhtmasqnre ekkkcyqpkd mvsffisipt
      661 nykklvqstt lradkhrkka ktlmlhnf
//
LOCUS       XP_076546314             673 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546314
VERSION     XP_076546314.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690199.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..673
                     /product="protein RUFY3 isoform X5"
                     /calculated_mol_wt=76989
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..662
                     /region_name="FYVE_RUFY1_like"
                     /note="FYVE domain found in RUN and FYVE domain-containing
                     protein RUFY1, RUFY2 and similar proteins; cd15721"
                     /db_xref="CDD:277261"
     Site            order(605,608,626..631,633..634,656..658)
                     /site_type="other"
                     /note="putative phosphatidylinositol 3-phosphate binding
                     site [chemical binding]"
                     /db_xref="CDD:277261"
     CDS             1..673
                     /gene="LOC117609905"
                     /coded_by="XM_076690199.1:1067..3088"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkhhc rncgkifcna csdnttalst stkpvrvcde
      661 cyvflvgrys sft
//
LOCUS       XP_076546315             652 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076546315
VERSION     XP_076546315.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690200.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..652
                     /product="protein RUFY3 isoform X6"
                     /calculated_mol_wt=74819
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..652
                     /gene="LOC117609905"
                     /coded_by="XM_076690200.1:1067..3025"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkklv qsttlradkh rkkaktlmlh nf
//
LOCUS       XP_076546316             646 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546316
VERSION     XP_076546316.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690201.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="protein RUFY3 isoform X7"
                     /calculated_mol_wt=74061
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..646
                     /gene="LOC117609905"
                     /coded_by="XM_076690201.1:1066..3006"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkila hngvseqrre kkmlpt
//
LOCUS       XP_076546317             646 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546317
VERSION     XP_076546317.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690202.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="protein RUFY3 isoform X7"
                     /calculated_mol_wt=74061
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..646
                     /gene="LOC117609905"
                     /coded_by="XM_076690202.1:1066..3006"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkila hngvseqrre kkmlpt
//
LOCUS       XP_076546318             643 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_076546318
VERSION     XP_076546318.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690203.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..643
                     /product="protein RUFY3 isoform X8"
                     /calculated_mol_wt=73281
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..643
                     /gene="LOC117609905"
                     /coded_by="XM_076690203.1:1066..2997"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkage gakpgtpatg nvt
//
LOCUS       XP_076546319             630 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_076546319
VERSION     XP_076546319.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690204.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="protein RUFY3 isoform X9"
                     /calculated_mol_wt=72228
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..630
                     /gene="LOC117609905"
                     /coded_by="XM_076690204.1:1065..2957"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkien
//
LOCUS       XP_076546320             630 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_076546320
VERSION     XP_076546320.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690205.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="protein RUFY3 isoform X10"
                     /calculated_mol_wt=72303
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..630
                     /gene="LOC117609905"
                     /coded_by="XM_076690205.1:1065..2957"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkmnw
//
LOCUS       XP_076546321             630 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_076546321
VERSION     XP_076546321.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690206.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="protein RUFY3 isoform X11"
                     /calculated_mol_wt=72266
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..630
                     /gene="LOC117609905"
                     /coded_by="XM_076690206.1:1065..2957"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkekh
//
LOCUS       XP_076546322             629 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X12 [Osmia lignaria lignaria].
ACCESSION   XP_076546322
VERSION     XP_076546322.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690207.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="protein RUFY3 isoform X12"
                     /calculated_mol_wt=72098
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..629
                     /gene="LOC117609905"
                     /coded_by="XM_076690207.1:1065..2954"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkil
//
LOCUS       XP_076546323             628 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X13 [Osmia lignaria lignaria].
ACCESSION   XP_076546323
VERSION     XP_076546323.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690208.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..628
                     /product="protein RUFY3 isoform X13"
                     /calculated_mol_wt=72003
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <291..376
                     /region_name="Leu_zip"
                     /note="Leucine zipper; pfam15294"
                     /db_xref="CDD:464620"
     Region          <362..>610
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          605..>627
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     CDS             1..628
                     /gene="LOC117609905"
                     /coded_by="XM_076690208.1:1064..2950"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq ecegslkhkt elitkleakt lsmtetiqkm dekckemdgv
      481 rsgaekrvri lgveaaerea rvtgverelr lerewrtslq easicnaeki sqlhqeidql
      541 rrvsekylal qeehyalkei cseqertlee lggqlstakl vavelreaad naqqqqqqqd
      601 gaatwandrl vshckscnre fnitrrkm
//
LOCUS       XP_076546324             618 aa            linear   INV 03-SEP-2025
DEFINITION  protein RUFY3 isoform X14 [Osmia lignaria lignaria].
ACCESSION   XP_076546324
VERSION     XP_076546324.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690209.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..618
                     /product="protein RUFY3 isoform X14"
                     /calculated_mol_wt=70115
     Region          87..242
                     /region_name="RUN_RUFY1_like"
                     /note="RUN domain found in RUN and FYVE domain-containing
                     proteins, RUFY1, RUFY2, RUFY3 and similar proteins;
                     cd17681"
                     /db_xref="CDD:439043"
     Region          <284..474
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..618
                     /gene="LOC117609905"
                     /coded_by="XM_076690209.1:1063..2919"
                     /db_xref="GeneID:117609905"
ORIGIN      
        1 mrqesqnglk irppgdhggv hhggfmfeed magaqdtiyl cnfrvsvdge wlclkelqdv
       61 dfslqdsmqr tpspplalsa rdpviiersn lvnisklivk elietslkyg rmldsdhmpl
      121 qhffivlehv lrhglrpkkg ilgpkkelwd ilqlvekycp eaqditssir dlptvrtamg
      181 rarawlrmal mqkkladylk vlidhkedil seyfepdalm mseeaivimg llvglnvidc
      241 nlcvkeedld cqqgvidfsi ylrnsnhipg espddelend nmttvldqkn yieelnrhln
      301 atvtnlqakv esltttnalm kedlaiaknn ilslheenrq lkkelgievk dtnengkppi
      361 kitettaeie elrsrleaek kmrqdvekel elqismksem evamkllekd ihekqdtiis
      421 lrrqldeikl inlemykklq eceheltqkg emvsrlhakt nqigkilhnl ekynhlmkdg
      481 enirspttps svsksilnkt sptsprgekl trlsssdnvi ttvhqqnsst nqqqspnsqh
      541 qsantpqlss sngqqcnnqq qsadnqqdss snneqmevns qlqnanskel tksenilqen
      601 kstneipfna ltqekana
//
LOCUS       XP_034181871             822 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605090 [Osmia lignaria lignaria].
ACCESSION   XP_034181871
VERSION     XP_034181871.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181871.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..822
                     /product="uncharacterized protein LOC117605090"
                     /calculated_mol_wt=92977
     Region          141..166
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(148..150,152..153,157,165,172,175..179,181..182)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          167..189
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          201..218
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..822
                     /gene="LOC117605090"
                     /coded_by="XM_034325980.2:189..2657"
                     /db_xref="GeneID:117605090"
ORIGIN      
        1 marmfqyvrp vdavltsvtl gtrpdhseng irikeeppdl dyddlnyllt danttaqnvs
       61 tkskqnhldv vnnenneril rprsvcsnkd ssyftrkkrk htlndsselk dsqpakkprl
      121 ylrdilplqv ednlmiptrt ckicfvsfks vqslsvhski yntiliceic ntpfnsrpql
      181 qrhischfht tfmssrksir cwfcnrsfce kkrlqmhlfh chrtlledey slqtdidkil
      241 enmeqlcrvk rhshisnsst gvttdtdkie kntcredtni rvtrnsdkvr rnmetsmtsv
      301 indvernipm kevftmpvtn eiyqtknnkr vddamvripr daikiekdtw avdamisvtd
      361 dveildetrv taledilnii gvvddteksv piedranrii ndadkieenr rmedsmeavr
      421 gtnditnmaa vqpcsnndaa svaggtnyaa diigetsdit smvnvtndvd kieesanpit
      481 dsvtqnvdqv eqntpvegsa nhvisdniki esntsikeit dsvidhisqn tteasesrvt
      541 tninktqrnv ptvksvghek kvvqktrqtq kpiastnspt klntsspgkk kmrqttltef
      601 lsfsnksfke ddiklekvdi pqesfdasar nasrnltfgr tstpipnsta vnfstpntpl
      661 lkmhispnsl lgllndeiks epvesfnsai aqnspynfrk sthdsrrssv dsrdatpssv
      721 asrrsrstnt skqikrtttp leerwknkyk ckecvvrler cdyvskrvll ktdrsesiip
      781 lqltigsaeg tarngdvikp snpririvnf dncivvelle sk
//
LOCUS       XP_034181882             822 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605090 [Osmia lignaria lignaria].
ACCESSION   XP_034181882
VERSION     XP_034181882.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325991.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181882.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..822
                     /product="uncharacterized protein LOC117605090"
                     /calculated_mol_wt=92977
     Region          141..166
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(148..150,152..153,157,165,172,175..179,181..182)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          167..189
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Region          201..218
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..822
                     /gene="LOC117605090"
                     /coded_by="XM_034325991.2:143..2611"
                     /db_xref="GeneID:117605090"
ORIGIN      
        1 marmfqyvrp vdavltsvtl gtrpdhseng irikeeppdl dyddlnyllt danttaqnvs
       61 tkskqnhldv vnnenneril rprsvcsnkd ssyftrkkrk htlndsselk dsqpakkprl
      121 ylrdilplqv ednlmiptrt ckicfvsfks vqslsvhski yntiliceic ntpfnsrpql
      181 qrhischfht tfmssrksir cwfcnrsfce kkrlqmhlfh chrtlledey slqtdidkil
      241 enmeqlcrvk rhshisnsst gvttdtdkie kntcredtni rvtrnsdkvr rnmetsmtsv
      301 indvernipm kevftmpvtn eiyqtknnkr vddamvripr daikiekdtw avdamisvtd
      361 dveildetrv taledilnii gvvddteksv piedranrii ndadkieenr rmedsmeavr
      421 gtnditnmaa vqpcsnndaa svaggtnyaa diigetsdit smvnvtndvd kieesanpit
      481 dsvtqnvdqv eqntpvegsa nhvisdniki esntsikeit dsvidhisqn tteasesrvt
      541 tninktqrnv ptvksvghek kvvqktrqtq kpiastnspt klntsspgkk kmrqttltef
      601 lsfsnksfke ddiklekvdi pqesfdasar nasrnltfgr tstpipnsta vnfstpntpl
      661 lkmhispnsl lgllndeiks epvesfnsai aqnspynfrk sthdsrrssv dsrdatpssv
      721 asrrsrstnt skqikrtttp leerwknkyk ckecvvrler cdyvskrvll ktdrsesiip
      781 lqltigsaeg tarngdvikp snpririvnf dncivvelle sk
//
LOCUS       XP_034181918             724 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605110 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181918
VERSION     XP_034181918.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326027.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..724
                     /product="uncharacterized protein LOC117605110 isoform X1"
                     /calculated_mol_wt=80491
     Region          <64..>218
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          64..88
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          89..112
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          113..136
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          137..160
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          161..184
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          185..208
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          217..>258
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          270..374
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          286..290
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          299..303
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          340..344
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          354..359
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          367..370
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..724
                     /gene="LOC117605110"
                     /coded_by="XM_034326027.2:1..2175"
                     /db_xref="GeneID:117605110"
ORIGIN      
        1 mcskmrawww rgallgtmml lswtsssegc psmctckwks gkewvecanr dlkglpqgar
       61 eetqvldlss nhlvslppec fqalglinlq rlylsrshis riasrafvgl vglveldlse
      121 nlieeiptet fpfysnlmkl llngnpikev rrgafqnlph ltnlelsqca lesveqgsfd
      181 glhllewlrl dgnrltrvpe htlplggslr gltlhnnpwl cdcrlrptqa wlkesapaap
      241 qesepvcdap prlrgkqika ikvnelaclp hielqervea yegdnvtlkc dvyavpaakl
      301 awwfngepce lqnendsisv sspmfpryvy rqrggtnmss tlllysvetl negtytciae
      361 ngagsaeanl slrvlfqeki tveppndrsr sgyvvaiaag alvgtlfalg sligsivfcm
      421 rkrrrdrkrn skalvsqnks vmpitkdttt slpcrkgngs liglehqqmv syteremnra
      481 atlerrehrn leepycspvs kyltepdlin evpettdvgy gqlyrhqpge rqileydsgy
      541 plqpdlrppn ipqlsyldqd gyplnfglpk ipfsaastlp rlrqrmpveg savapparys
      601 reaeflarsp gydpvlprtd arytaegypy ppqqqqpiqp veqplqqqlp vspvspvavf
      661 pevpfipspp aayrgettpl sprsllskta reaaaaaaar aedlqpphhp espdegyvgd
      721 amdv
//
LOCUS       XP_034181927             689 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605110 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181927
VERSION     XP_034181927.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326036.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..689
                     /product="uncharacterized protein LOC117605110 isoform X2"
                     /calculated_mol_wt=76878
     Region          <64..>218
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          64..88
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          89..112
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          113..136
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          137..160
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          161..184
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          185..208
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          217..>258
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          270..374
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          286..290
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          299..303
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          340..344
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          354..359
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          367..370
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..689
                     /gene="LOC117605110"
                     /coded_by="XM_034326036.2:1..2070"
                     /db_xref="GeneID:117605110"
ORIGIN      
        1 mcskmrawww rgallgtmml lswtsssegc psmctckwks gkewvecanr dlkglpqgar
       61 eetqvldlss nhlvslppec fqalglinlq rlylsrshis riasrafvgl vglveldlse
      121 nlieeiptet fpfysnlmkl llngnpikev rrgafqnlph ltnlelsqca lesveqgsfd
      181 glhllewlrl dgnrltrvpe htlplggslr gltlhnnpwl cdcrlrptqa wlkesapaap
      241 qesepvcdap prlrgkqika ikvnelaclp hielqervea yegdnvtlkc dvyavpaakl
      301 awwfngepce lqnendsisv sspmfpryvy rqrggtnmss tlllysvetl negtytciae
      361 ngagsaeanl slrvlfqeki tveppndrsr sgyvvaiaag alvgtlfalg sligsivfcm
      421 rkrrrdrkrn skalvsqnks vmpitkdttt slpcrkgngs liglehqqmv syteremnra
      481 atlerrehrn leepycspvs kyltepdlin evpettdvgy gqlyrhqpge rqileydsgy
      541 plqpdlrppn ipqlsyldqd gyplnfglpk ipfsaastlp rlrqrmpveg savapparys
      601 reaeflarsp gydpvlprtd arytaegypy ppqqqqpiqp veqplqqqlp vspvspvavf
      661 pevpfipspp aayrgerpvs aslaenegr
//
LOCUS       XP_034189274            1621 aa            linear   INV 03-SEP-2025
DEFINITION  laminin subunit gamma-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189274
VERSION     XP_034189274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333383.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1621
                     /product="laminin subunit gamma-1 isoform X1"
                     /calculated_mol_wt=179739
     Region          54..282
                     /region_name="Laminin_N"
                     /note="Laminin N-terminal (Domain VI); pfam00055"
                     /db_xref="CDD:459653"
     Region          283..330
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(284,286,296,307,309,318)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          344..395
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(344,346,353,369,372,381)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          399..443
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(400,402,412,418,420,429)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          447..497
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(447,449,461,468,470,479)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          562..694
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          <695..722
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Region          729..771
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(730,732,739,746,748,757)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          830..874
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(830,832,844,851,854,863)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          886..938
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(886,888,904,911,913,922)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          941..986
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(941,943,953,960,962,971)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          988..1035
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(989,991,1001,1007,1009,1018)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1046..1601
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          <1325..1605
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1621
                     /gene="LanB2"
                     /coded_by="XM_034333383.2:1422..6287"
                     /db_xref="GeneID:117608361"
ORIGIN      
        1 mtrgkhkkil ilavicliav faepepsddl pdrfekksrd edkcyddngr prrcippfen
       61 aafdvlmeat ntcgqdrpte fckqtgvqnk sceicryedh saryltdrdn ndnstwwqse
      121 tmfegieypn qvnltlhlgk tfdityvrvl fesprpeswg iykrkndksp wepyqfysat
      181 crdtydlpds ketvqgddtr vlctsdysdi spltkgtvaf stlegrpsay yfetnpalqe
      241 wvqatglrit ldrlntfgde vfgddhvlks yyyaiadvav garcacngha gecvnstsvd
      301 grtrrvcrce hntagpdcne clpfyndapw gratttdahe ckpcncngys ercyfdkely
      361 katghgghcl dckanrdgan cercrenyyq rpqdnycvac ncneigsrnl qcnsegkcqc
      421 kpgvtgdkcd rcaanyynfg sygctscecn vagslentpn cdpisgvctc kenvegkrcr
      481 vckpgffnla tqnefgctpc fcyghssvcr patgyskivi esmfvrgaer wtatvagnpi
      541 plhydphtqt isataldrdn vyfvapdrfl gdqrasynqd lsftlrigea gptptardvi
      601 leggngeqit qpifgqqnri ptvtpqeyrf klhehpsygw eprlssrafm silsnltaik
      661 irgtythqgr gflddvklet ahrgaagepa dwvehcqcph gyvgqfcesc apgfhhdppn
      721 ggpfalcvpc ncnghadice aetgqcicqh ntagsncelc krgyyghplk gtqddckpcp
      781 cpdngpcill gnnpdpicse cpsgrtgprc etcsdgyfgn pekgiacksc dcnnnidlna
      841 vrncnhetge clkcvnntag fhceeclpgy ygdalselke dgcklcqcyp pgtvelddgr
      901 vapceqltgh ctckphvigr ncdkceegyy hilsgegcap cncdsegsyn rtcnpvtgqc
      961 ecrpgvtgqh cdacvpyqyg fgregckpcd cdsigsqdlq cdasgqcpcl qnvegrrcdr
     1021 ckenkhnrqn gcvdcpdcyn lvqsaanshr erlaelentl kkinssptvi kdsdfekelk
     1081 nvqdrvknil htakqgsgne nktlveqlde lrdqlneige isltvnltat dayktasegl
     1141 msideaekvl dkihgqltda edylatsgat alaeaksrae qvgqqnkqmt tiaqearvla
     1201 dfnvneakki htlaeqarnt tieayslakk aiakysnmtd dirglenkle llehrmnevk
     1261 nltsiaatks savsqeaidl lildlalppv didrlqsqve evnneglrlk eqaqllldqn
     1321 ehlinemtek vrkseelier aqdqqaatae lvaeldgane kandavkrgd qtlkeaqetl
     1381 kklgefhaev ererikaqda lkdigdiedl iinainqaaq aneiltgsed naklareraq
     1441 naqsyaeeas akanairiea nktkiealrv gneaeklhsr vdttdsmmkg yeiqisqdtn
     1501 itteanhkvg qakinvslas qqvdkalaev aeimnelenv peiddadlnr leerlvaaek
     1561 eiaaanldqr iraltdaknl qtqwvknyed evsrlrmeve nigdirkvlp vdchkrvrle
     1621 p
//
LOCUS       XP_076546326            1621 aa            linear   INV 03-SEP-2025
DEFINITION  laminin subunit gamma-1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546326
VERSION     XP_076546326.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690211.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1621
                     /product="laminin subunit gamma-1 isoform X1"
                     /calculated_mol_wt=179739
     Region          54..282
                     /region_name="Laminin_N"
                     /note="Laminin N-terminal (Domain VI); pfam00055"
                     /db_xref="CDD:459653"
     Region          283..330
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(284,286,296,307,309,318)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          344..395
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(344,346,353,369,372,381)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          399..443
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(400,402,412,418,420,429)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          447..497
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(447,449,461,468,470,479)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          562..694
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          <695..722
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Region          729..771
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(730,732,739,746,748,757)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          830..874
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(830,832,844,851,854,863)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          886..938
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(886,888,904,911,913,922)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          941..986
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(941,943,953,960,962,971)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          988..1035
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(989,991,1001,1007,1009,1018)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1046..1601
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          <1325..1605
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1621
                     /gene="LanB2"
                     /coded_by="XM_076690211.1:1499..6364"
                     /db_xref="GeneID:117608361"
ORIGIN      
        1 mtrgkhkkil ilavicliav faepepsddl pdrfekksrd edkcyddngr prrcippfen
       61 aafdvlmeat ntcgqdrpte fckqtgvqnk sceicryedh saryltdrdn ndnstwwqse
      121 tmfegieypn qvnltlhlgk tfdityvrvl fesprpeswg iykrkndksp wepyqfysat
      181 crdtydlpds ketvqgddtr vlctsdysdi spltkgtvaf stlegrpsay yfetnpalqe
      241 wvqatglrit ldrlntfgde vfgddhvlks yyyaiadvav garcacngha gecvnstsvd
      301 grtrrvcrce hntagpdcne clpfyndapw gratttdahe ckpcncngys ercyfdkely
      361 katghgghcl dckanrdgan cercrenyyq rpqdnycvac ncneigsrnl qcnsegkcqc
      421 kpgvtgdkcd rcaanyynfg sygctscecn vagslentpn cdpisgvctc kenvegkrcr
      481 vckpgffnla tqnefgctpc fcyghssvcr patgyskivi esmfvrgaer wtatvagnpi
      541 plhydphtqt isataldrdn vyfvapdrfl gdqrasynqd lsftlrigea gptptardvi
      601 leggngeqit qpifgqqnri ptvtpqeyrf klhehpsygw eprlssrafm silsnltaik
      661 irgtythqgr gflddvklet ahrgaagepa dwvehcqcph gyvgqfcesc apgfhhdppn
      721 ggpfalcvpc ncnghadice aetgqcicqh ntagsncelc krgyyghplk gtqddckpcp
      781 cpdngpcill gnnpdpicse cpsgrtgprc etcsdgyfgn pekgiacksc dcnnnidlna
      841 vrncnhetge clkcvnntag fhceeclpgy ygdalselke dgcklcqcyp pgtvelddgr
      901 vapceqltgh ctckphvigr ncdkceegyy hilsgegcap cncdsegsyn rtcnpvtgqc
      961 ecrpgvtgqh cdacvpyqyg fgregckpcd cdsigsqdlq cdasgqcpcl qnvegrrcdr
     1021 ckenkhnrqn gcvdcpdcyn lvqsaanshr erlaelentl kkinssptvi kdsdfekelk
     1081 nvqdrvknil htakqgsgne nktlveqlde lrdqlneige isltvnltat dayktasegl
     1141 msideaekvl dkihgqltda edylatsgat alaeaksrae qvgqqnkqmt tiaqearvla
     1201 dfnvneakki htlaeqarnt tieayslakk aiakysnmtd dirglenkle llehrmnevk
     1261 nltsiaatks savsqeaidl lildlalppv didrlqsqve evnneglrlk eqaqllldqn
     1321 ehlinemtek vrkseelier aqdqqaatae lvaeldgane kandavkrgd qtlkeaqetl
     1381 kklgefhaev ererikaqda lkdigdiedl iinainqaaq aneiltgsed naklareraq
     1441 naqsyaeeas akanairiea nktkiealrv gneaeklhsr vdttdsmmkg yeiqisqdtn
     1501 itteanhkvg qakinvslas qqvdkalaev aeimnelenv peiddadlnr leerlvaaek
     1561 eiaaanldqr iraltdaknl qtqwvknyed evsrlrmeve nigdirkvlp vdchkrvrle
     1621 p
//
LOCUS       XP_034189283            1313 aa            linear   INV 03-SEP-2025
DEFINITION  laminin subunit gamma-1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034189283
VERSION     XP_034189283.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333392.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1313
                     /product="laminin subunit gamma-1 isoform X2"
                     /calculated_mol_wt=144659
     Region          36..87
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(36,38,45,61,64,73)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          91..135
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(92,94,104,110,112,121)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          139..189
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(139,141,153,160,162,171)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          254..386
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          <387..414
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Region          421..463
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(422,424,431,438,440,449)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          522..566
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(522,524,536,543,546,555)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          578..630
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(578,580,596,603,605,614)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          633..678
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(633,635,645,652,654,663)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          680..727
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(681,683,693,699,701,710)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          738..1293
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          <1017..1297
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1313
                     /gene="LanB2"
                     /coded_by="XM_034333392.2:146..4087"
                     /db_xref="GeneID:117608361"
ORIGIN      
        1 melkgrliac lmlvnmvice ssrwdgilpw ysgstcncng ysercyfdke lykatghggh
       61 cldckanrdg ancercreny yqrpqdnycv acncneigsr nlqcnsegkc qckpgvtgdk
      121 cdrcaanyyn fgsygctsce cnvagslent pncdpisgvc tckenvegkr crvckpgffn
      181 latqnefgct pcfcyghssv crpatgyski viesmfvrga erwtatvagn piplhydpht
      241 qtisataldr dnvyfvapdr flgdqrasyn qdlsftlrig eagptptard vileggngeq
      301 itqpifgqqn riptvtpqey rfklhehpsy gweprlssra fmsilsnlta ikirgtythq
      361 grgflddvkl etahrgaage padwvehcqc phgyvgqfce scapgfhhdp pnggpfalcv
      421 pcncnghadi ceaetgqcic qhntagsnce lckrgyyghp lkgtqddckp cpcpdngpci
      481 llgnnpdpic secpsgrtgp rcetcsdgyf gnpekgiack scdcnnnidl navrncnhet
      541 geclkcvnnt agfhceeclp gyygdalsel kedgcklcqc yppgtveldd grvapceqlt
      601 ghctckphvi grncdkceeg yyhilsgegc apcncdsegs ynrtcnpvtg qcecrpgvtg
      661 qhcdacvpyq ygfgregckp cdcdsigsqd lqcdasgqcp clqnvegrrc drckenkhnr
      721 qngcvdcpdc ynlvqsaans hrerlaelen tlkkinsspt vikdsdfeke lknvqdrvkn
      781 ilhtakqgsg nenktlveql delrdqlnei geisltvnlt atdayktase glmsideaek
      841 vldkihgqlt daedylatsg atalaeaksr aeqvgqqnkq mttiaqearv ladfnvneak
      901 kihtlaeqar nttieaysla kkaiakysnm tddirglenk lellehrmne vknltsiaat
      961 kssavsqeai dllildlalp pvdidrlqsq veevnneglr lkeqaqllld qnehlinemt
     1021 ekvrkseeli eraqdqqaat aelvaeldga nekandavkr gdqtlkeaqe tlkklgefha
     1081 evererikaq dalkdigdie dliinainqa aqaneiltgs ednaklarer aqnaqsyaee
     1141 asakanairi eanktkieal rvgneaeklh srvdttdsmm kgyeiqisqd tnitteanhk
     1201 vgqakinvsl asqqvdkala evaeimnele nvpeiddadl nrleerlvaa ekeiaaanld
     1261 qriraltdak nlqtqwvkny edevsrlrme venigdirkv lpvdchkrvr lep
//
LOCUS       XP_034189498             489 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase activating protein at 68F isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189498
VERSION     XP_034189498.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333607.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="rho GTPase activating protein at 68F isoform X1"
                     /calculated_mol_wt=55282
     Region          135..270
                     /region_name="CRAL_TRIO_2"
                     /note="Divergent CRAL/TRIO domain; pfam13716"
                     /db_xref="CDD:463965"
     Region          293..487
                     /region_name="RhoGAP-p50rhoGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain of p50RhoGAP-like proteins;
                     p50RhoGAP, also known as RhoGAP-1, contains a C-terminal
                     RhoGAP domain and an N-terminal Sec14 domain which binds
                     phosphatidylinositol 3,4; cd04404"
                     /db_xref="CDD:239869"
     Site            order(338,373,377,446,449..450,473)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:239869"
     Site            338
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239869"
     CDS             1..489
                     /gene="RhoGAP68F"
                     /coded_by="XM_034333607.2:289..1758"
                     /db_xref="GeneID:117608449"
ORIGIN      
        1 meadyqptls ptrtltaian dvedpypsls dyhdyepnle fddtelqsts nnavqlleek
       61 ldlissgagt gidylespvs dgtieenfee alvdapvies aedlaaldge ladeedyldi
      121 arhgivevvg ddsagrkiiv vsacklppvg ketfnhakll rylthtldtf veqdysliyf
      181 hygltsknkp plswlwqayk afdrkykknl kalylvhptn firivwqifk paisakfgrk
      241 mmyvnyleel aqyinldqli ippqviehne qlmlknkknl ptsppqstvt tpvgttqfga
      301 sllfikennn gdpipaivrq cvefldtpda letegifrrs anvavikelq srcnqglpvd
      361 fqgdphiaav llktflreld eplmtyelyd eitqfqalsk derprrvkil vleklpedny
      421 qllkyivqfl srvmdrcdln kmtssnlavv fgpnlvrapp srgmslsaig pinqfidflf
      481 ihqdkifii
//
LOCUS       XP_034189504             441 aa            linear   INV 03-SEP-2025
DEFINITION  rho GTPase activating protein at 68F isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189504
VERSION     XP_034189504.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333613.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="rho GTPase activating protein at 68F isoform X2"
                     /calculated_mol_wt=50236
     Region          87..222
                     /region_name="CRAL_TRIO_2"
                     /note="Divergent CRAL/TRIO domain; pfam13716"
                     /db_xref="CDD:463965"
     Region          245..439
                     /region_name="RhoGAP-p50rhoGAP"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain of p50RhoGAP-like proteins;
                     p50RhoGAP, also known as RhoGAP-1, contains a C-terminal
                     RhoGAP domain and an N-terminal Sec14 domain which binds
                     phosphatidylinositol 3,4; cd04404"
                     /db_xref="CDD:239869"
     Site            order(290,325,329,398,401..402,425)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:239869"
     Site            290
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239869"
     CDS             1..441
                     /gene="RhoGAP68F"
                     /coded_by="XM_034333613.2:290..1615"
                     /db_xref="GeneID:117608449"
ORIGIN      
        1 meadyqptls ptrtltaian dvedpypsls dyhdyepnle fddtelqsts nnaedlaald
       61 geladeedyl diarhgivev vgddsagrki ivvsacklpp vgketfnhak llrylthtld
      121 tfveqdysli yfhygltskn kpplswlwqa ykafdrkykk nlkalylvhp tnfirivwqi
      181 fkpaisakfg rkmmyvnyle elaqyinldq liippqvieh neqlmlknkk nlptsppqst
      241 vttpvgttqf gasllfiken nngdpipaiv rqcvefldtp daletegifr rsanvavike
      301 lqsrcnqglp vdfqgdphia avllktflre ldeplmtyel ydeitqfqal skderprrvk
      361 ilvleklped nyqllkyivq flsrvmdrcd lnkmtssnla vvfgpnlvra ppsrgmslsa
      421 igpinqfidf lfihqdkifi i
//
LOCUS       XP_034189531             397 aa            linear   INV 03-SEP-2025
DEFINITION  SIN3-HDAC complex-associated factor [Osmia lignaria lignaria].
ACCESSION   XP_034189531
VERSION     XP_034189531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333640.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="SIN3-HDAC complex-associated factor"
                     /calculated_mol_wt=43187
     Region          2..220
                     /region_name="FAM60A"
                     /note="Protein Family FAM60A; pfam15396"
                     /db_xref="CDD:464703"
     CDS             1..397
                     /gene="LOC117608464"
                     /coded_by="XM_034333640.2:543..1736"
                     /db_xref="GeneID:117608464"
ORIGIN      
        1 mfsfhkpkvy rsstgccick akssssrftd skkyeddfie cfqleerrtg eicnacvllv
       61 krwkklpags nrnwrhvvda ragpgikslt kfksknkkki kdlpekfeki mkkkhmylkt
      121 drdreqspam sddltedylh gdgskgssra gspvgsddip vtekpmdmqd dteskddltv
      181 ngfidltyfk reiiccgtif kgpygevivd pslikpcigc iarqqrqqqt nalgcspvhs
      241 sasaspvhss asaspahsve sgteatvskq tsktfsdsss dsgydessnq gvgeskitki
      301 iqntsatvkp avkiqplksv qlkaipvsea vrlktdvpik lvpikqidql sckplslvss
      361 anvtlgssts hsivtvptnp lvdfamhaas srqsvsn
//
LOCUS       XP_034189541             397 aa            linear   INV 03-SEP-2025
DEFINITION  SIN3-HDAC complex-associated factor [Osmia lignaria lignaria].
ACCESSION   XP_034189541
VERSION     XP_034189541.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333650.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="SIN3-HDAC complex-associated factor"
                     /calculated_mol_wt=43187
     Region          2..220
                     /region_name="FAM60A"
                     /note="Protein Family FAM60A; pfam15396"
                     /db_xref="CDD:464703"
     CDS             1..397
                     /gene="LOC117608464"
                     /coded_by="XM_034333650.2:132..1325"
                     /db_xref="GeneID:117608464"
ORIGIN      
        1 mfsfhkpkvy rsstgccick akssssrftd skkyeddfie cfqleerrtg eicnacvllv
       61 krwkklpags nrnwrhvvda ragpgikslt kfksknkkki kdlpekfeki mkkkhmylkt
      121 drdreqspam sddltedylh gdgskgssra gspvgsddip vtekpmdmqd dteskddltv
      181 ngfidltyfk reiiccgtif kgpygevivd pslikpcigc iarqqrqqqt nalgcspvhs
      241 sasaspvhss asaspahsve sgteatvskq tsktfsdsss dsgydessnq gvgeskitki
      301 iqntsatvkp avkiqplksv qlkaipvsea vrlktdvpik lvpikqidql sckplslvss
      361 anvtlgssts hsivtvptnp lvdfamhaas srqsvsn
//
LOCUS       XP_034189567             281 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin-6 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189567
VERSION     XP_034189567.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333676.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..281
                     /product="syntaxin-6 isoform X1"
                     /calculated_mol_wt=31900
     Region          6..116
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          174..239
                     /region_name="SNARE_Syntaxin6"
                     /note="SNARE motif of syntaxin 6; cd15851"
                     /db_xref="CDD:277204"
     Site            order(178..181,183..185,187..189,191..192,194..199,
                     201..202,204..205,208..209,211..213,215..217,219..220,
                     222..223,226..227,229..230,232)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277204"
     Site            205
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277204"
     CDS             1..281
                     /gene="LOC117608477"
                     /coded_by="XM_034333676.2:250..1095"
                     /db_xref="GeneID:117608477"
ORIGIN      
        1 mtlenpffvv kdevckalnk nrglykrwte lqdvvvtspn vsggipisrd elewtttelr
       61 kalrsiewdl ddledtiriv eknptkfkid nkeltvqrrf ieetreevki mkdkmnlsrg
      121 rdrdstarqp lldnsparvp vnhgttkysk leneidspnr qflgdtlqqq ndmmrqqdeq
      181 ldmigesigt lktvsrqint eldeqavmld efgnelevtd skldatmkkm akvlhmsngn
      241 ynnyipaptt atssvsdlas kpsslsflsn titncflcsg d
//
LOCUS       XP_034189576             263 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin-6 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034189576
VERSION     XP_034189576.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333685.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="syntaxin-6 isoform X2"
                     /calculated_mol_wt=30352
     Region          6..116
                     /region_name="SNARE_NTD_STX6_STX10"
                     /note="N-terminal domain of syntaxin-6, syntaxin-10, and
                     similar proteins; cd21443"
                     /db_xref="CDD:410569"
     Region          174..239
                     /region_name="SNARE_Syntaxin6"
                     /note="SNARE motif of syntaxin 6; cd15851"
                     /db_xref="CDD:277204"
     Site            order(178..181,183..185,187..189,191..192,194..199,
                     201..202,204..205,208..209,211..213,215..217,219..220,
                     222..223,226..227,229..230,232)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277204"
     Site            205
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277204"
     CDS             1..263
                     /gene="LOC117608477"
                     /coded_by="XM_034333685.2:249..1040"
                     /db_xref="GeneID:117608477"
ORIGIN      
        1 mtlenpffvv kdevckalnk nrglykrwte lqdvvvtspn vsggipisrd elewtttelr
       61 kalrsiewdl ddledtiriv eknptkfkid nkeltvqrrf ieetreevki mkdkmnlsrg
      121 rdrdstarqp lldnsparvp vnhgttkysk leneidspnr qflgdtlqqq ndmmrqqdeq
      181 ldmigesigt lktvsrqint eldeqavmld efgnelevtd skldatmkkm akvlhmsndr
      241 rqwvaigvlt ailvfliilf iik
//
LOCUS       XP_034189590             222 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab21 [Osmia lignaria lignaria].
ACCESSION   XP_034189590
VERSION     XP_034189590.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333699.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="RAS oncogene family member Rab21"
                     /calculated_mol_wt=24571
     Region          14..175
                     /region_name="Rab21"
                     /note="Rab GTPase family 21 (Rab21); cd04123"
                     /db_xref="CDD:133323"
     Site            14..15
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:133323"
     Site            20..27
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133323"
     Site            order(22,25..28,38,44..45,126..127,129,156..158)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133323"
     Site            order(28..38,43..44)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:133323"
     Site            order(38,43..51)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133323"
     Site            order(43,47..54,61,63)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:133323"
     Site            45
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133323"
     Site            order(46,48..50,65,67,74..75,78,82,84..87)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:133323"
     Site            order(46..47,49,67..68,75,77,79..81)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:133323"
     Site            46..50
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:133323"
     Site            63..67
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:133323"
     Site            68..71
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133323"
     Site            order(71,73..83)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133323"
     Site            74..79
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:133323"
     Site            82..86
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:133323"
     Site            91..96
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:133323"
     Site            119..123
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:133323"
     Site            126..129
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133323"
     Site            156..158
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133323"
     Site            172..175
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:133323"
     CDS             1..222
                     /gene="Rab21"
                     /coded_by="XM_034333699.2:440..1108"
                     /db_xref="GeneID:117608483"
ORIGIN      
        1 massvsstgn gynfkvvllg egcvgktsva lryvdgkfnd khistlqasf lnkkltvndk
       61 kvnlaiwdta gqekfhalgp iyyrmsngai lvyditdedt fqkvknwvke lkkmlgseic
      121 laiagnkvdl eknrsvsiqe aeeyakqvga mhyhtsakls qnieemfldl tqrmiqhade
      181 aeqkstltrt nstrrnvvvv edeaeerqya kssccggssq as
//
LOCUS       XP_034192554             254 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609902 [Osmia lignaria lignaria].
ACCESSION   XP_034192554
VERSION     XP_034192554.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336663.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192554.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="uncharacterized protein LOC117609902"
                     /calculated_mol_wt=28305
     Region          <3..101
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Region          <179..217
                     /region_name="zf-RVT"
                     /note="zinc-binding in reverse transcriptase; pfam13966"
                     /db_xref="CDD:433612"
     CDS             1..254
                     /gene="LOC117609902"
                     /coded_by="XM_034336663.2:1..765"
                     /db_xref="GeneID:117609902"
ORIGIN      
        1 mtyklghhvt ffqaevfaiw acakynlekd hsgkhiyics dsiaalgaih kvevgsklvk
       61 dciltlsqls lnnrvkllwv pghagipgne ranelarlga tssqpcheyp igvstyalkg
      121 lakdwlnqef trlwnnangm rharalfegp sqklgdtlih ldrarlcmlv glvtghwytr
      181 khlarmrlte etlcprceee detplhillr crelralrgs ilgtlelssl sisaglvpvl
      241 lnfateaqlp rysq
//
LOCUS       XP_076546328             658 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305685 [Osmia lignaria lignaria].
ACCESSION   XP_076546328
VERSION     XP_076546328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690213.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..658
                     /product="uncharacterized protein LOC143305685"
                     /calculated_mol_wt=75047
     Region          142..>255
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Region          391..513
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(395..398,423..425,428,452,491,509)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..658
                     /gene="LOC143305685"
                     /coded_by="XM_076690213.1:1..1977"
                     /db_xref="GeneID:143305685"
ORIGIN      
        1 mkevwdkina irgnrkgcgt hfimnnqndv vsslqqisdi faltfannss tmnydedfrq
       61 ykgnreeeti nvtddlnhpf lntpiserel neaisrskns spgpdnipni fikilppigm
      121 killdifnii wsnhvfpkkw neaivipiak pgkdsskpnn yrpisltcnm ckileriinf
      181 rlrwflernn lltdnqfgsr tghstishlv sletrireaf ankqhvlavs ldiekacdmv
      241 wrykillnlk emglristgs fhtspigsil ieagepslqh rrkllsikys ikaatsrngi
      301 ihddifpknq srvncqhgnd ppwlriqnyl dninytlptm eknrfhkdsp wllqipvvdt
      361 slklgevmdl sdedwrgrfn afyanypsfk hiyvdgsknn lgtgasvftq dvsinhglns
      421 fnsifsaesy allmslnfia nspdskfiif sdslstlarc hnfrqtiemy vaiqelyndl
      481 insgkttiia wipahkgiyg nemadlgdkm attlrpnnha iynstlditn higsscelew
      541 nkswknykas plhksinnfy dlktnsefnr keqvaitrir sghtrlthac ilnggprmvc
      601 evcnvmlsid hiitkcdkyn lgrlkfnied dlknslvsvk yqetiiefck nigilnsl
//
LOCUS       XP_076546329             595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305686 [Osmia lignaria lignaria].
ACCESSION   XP_076546329
VERSION     XP_076546329.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690214.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="uncharacterized protein LOC143305686"
                     /calculated_mol_wt=67764
     Region          247..508
                     /region_name="RVT_2"
                     /note="Reverse transcriptase (RNA-dependent DNA
                     polymerase); pfam07727"
                     /db_xref="CDD:400190"
     CDS             1..595
                     /gene="LOC143305686"
                     /coded_by="XM_076690214.1:1..1788"
                     /db_xref="GeneID:143305686"
ORIGIN      
        1 mkeqdsrgng qmspysvwpf giilggsgvr ielhqesmpy kksgednavs smkgkfelrs
       61 regifvgyae qskgyriwip terkieisrd arfleklprk peesirdskp veitvqegra
      121 deelpketis idyessmdna peeegqpqhd ngefqtetpk rgpgrprilr tglrgrprkl
      181 fhpragetaq gnddvrndgk ssedastrde ieiactaeip vkyamsgder dewrlamaee
      241 vraivknnaw dvvdrstcrl tvggrfvlrn kynpdgslqr rkarlvaqgf tqqpgvdffe
      301 sfapvarlss irlvtalaaq ygmkirqfdv staylngale edvfmnipkf feealeeatr
      361 iepdvnirrr aikmlddira gnkvfhlkka lyglrqagrs wyreldrklr dlgakpsnad
      421 lciyrmnree dvviiavyvd dilvaarrld lilqvkdrlr ksleindlge vercvgiefa
      481 rddkkilihq kgyirdllky ygmsecrpgq srgreatfsr tdrqsyvsgg ryearhcphg
      541 elsqpisnvl rqvtldggek gteipamhnr vrdrlrtggr tndgirrrrl gqlpd
//
LOCUS       XP_034179446             319 aa            linear   INV 03-SEP-2025
DEFINITION  calaxin-like [Osmia lignaria lignaria].
ACCESSION   XP_034179446
VERSION     XP_034179446.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323555.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179446.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..319
                     /product="calaxin-like"
                     /calculated_mol_wt=36104
     Region          220..293
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     CDS             1..319
                     /gene="LOC117603936"
                     /coded_by="XM_034323555.2:1..960"
                     /db_xref="GeneID:117603936"
ORIGIN      
        1 mfenllfdll tnsrrmsddr slsssakprm ergksvgvgv gvgvgaagts aanlragatl
       61 mksvsiflms grkasvnskr qtsgspkhel rkrhrrrmpe vgatrnsaht vklieslkrk
      121 trfswseldn lcklykkltn ssqqqvgqsv itsrrsqpnq viegidrnif rdllhntfkv
      181 itedtlveri fccwdreneg iirlepwimg ldlylrgslr ekiefcfkvy dlnndgfitk
      241 deifqlfknc lmkqpgeedp deavrdlsem alkkldvdrd gkisfqdykv avteepllle
      301 afgqclmtde nrasvlatl
//
LOCUS       XP_034179472             237 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117603944 [Osmia lignaria lignaria].
ACCESSION   XP_034179472
VERSION     XP_034179472.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323581.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="uncharacterized protein LOC117603944"
                     /calculated_mol_wt=26401
     CDS             1..237
                     /gene="LOC117603944"
                     /coded_by="XM_034323581.1:1..714"
                     /db_xref="GeneID:117603944"
ORIGIN      
        1 mskfvcific vsfmiisvpg sseekdgvlr kikagleyat nyletakdia dlvakslghk
       61 qqqkrgddnd akqkgsfgas nlvsaffrli gldsqkvtai avnsviflaq misslfelkp
      121 akenlardld dnndnlswnp anlitesknd kiqrllrqaq ddelpnqlie kvdgvdsaci
      181 rlllcktspv iravqnflrn ktrteshpmt swlpsrdrfe ensdecenth tdcslfs
//
LOCUS       XP_034179484             263 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor Or2-like [Osmia lignaria lignaria].
ACCESSION   XP_034179484
VERSION     XP_034179484.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323593.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="odorant receptor Or2-like"
                     /calculated_mol_wt=30026
     Region          <19..236
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..263
                     /gene="LOC117603949"
                     /coded_by="XM_034323593.2:295..1086"
                     /db_xref="GeneID:117603949"
ORIGIN      
        1 meeffsttsa vnlkraeiic yiltiifftg svlfalvplv tkkqmtpldt vyftlprdkq
       61 wgrsfiyaln mmqiinagss lfldmltiwi mwhvackfnl lamefmifee kklkfwikrh
      121 qdvlsyvnei nsiiaplavk stfavgidii vsglvathnl nlvestkfft vtifsllrfv
      181 acswvadtit ekarniaeki ydspwiytsp rmrkmallim qrsqksvaih aggilsawtl
      241 efcglvlyti vtylmtlrav irv
//
LOCUS       XP_034182741             965 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182741
VERSION     XP_034182741.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326850.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..965
                     /product="discoidin domain-containing receptor 2 isoform
                     X1"
                     /calculated_mol_wt=108602
     Region          40..192
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(81,112,120)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          252..364
                     /region_name="F5_F8_type_C"
                     /note="F5/8 type C domain; pfam00754"
                     /db_xref="CDD:459925"
     Region          650..956
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(661..664,667,669,692,694,724,740..743,799,803..804,
                     806,816..817)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     CDS             1..965
                     /gene="LOC117605469"
                     /coded_by="XM_034326850.2:398..3295"
                     /db_xref="GeneID:117605469"
ORIGIN      
        1 mvksfgffar raipachhlf lflalaattt avdlsqciap lgmesgaipd aditasstfd
       61 tgnvgpqlar lkvenlggaw cpknqitqea rewleidlht vhlitatatq grfgnglgve
      121 yaeaylleyw rprlgkwvry rdikgkeviq gntntylesk helepplwas kirflpyshh
      181 trtvcmrvel ygcywsdgvv sysmpqgdkr gngweffdat ydghwdgelr rglgqltdgr
      241 tgpdnfkmgy ydndrnqgwv gwrndtrgqp veikfefdkv refsaihiyc nnqftkdvqv
      301 fshvdilfsi ggkyytgepi tytymedkif essrnitikl hhrvgkyvkl rlhfssrwil
      361 isevtfdsdv ahgnftpeea pttespiqsd vfvekngaae gelpvstakh ddptymavvi
      421 gvltavilll avaiflivsr hrqrkcfasp mtgkapshlg stcatvekga almaytledd
      481 eryaggslpt lprdlgnrll divklddyqe pyqalkyapy ysystvvmem kdmmlnnkgs
      541 ninhsakpsp paplfnarss faisstavya ngavdtsydy avpelgtvpl lnqdgtgraa
      601 avstgttdqd sifsktssrs sktenkkspt qqevlsalkr rleqtsvplf prhrlrmlsk
      661 laegafgtvy vaeaegipey gstttvgkrl vavkfllpea sekekldfqr dvrilaaled
      721 rniarvlgac creepycvvm eylehgdlcq flkthitadd ahsmpigvkt lsfncliyma
      781 aqiasgmryl enlnfvhrdl atrnclvgka yhikisdfgt dnelyacdyy kvdgtvplpi
      841 rwmawesifl gkyttksdvw afavtlweil nlgrrvpyeh lsdeevvqsl rrlhratdcn
      901 dtggenncke nndnlfdylp qpiacskdiy dlmldcwrre eterptfrei smflqrknlg
      961 yapts
//
LOCUS       XP_034182749             944 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182749
VERSION     XP_034182749.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326858.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..944
                     /product="discoidin domain-containing receptor 2 isoform
                     X2"
                     /calculated_mol_wt=106473
     Region          40..192
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(81,112,120)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     Region          252..364
                     /region_name="F5_F8_type_C"
                     /note="F5/8 type C domain; pfam00754"
                     /db_xref="CDD:459925"
     Region          629..935
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(640..643,646,648,671,673,703,719..722,778,782..783,
                     785,795..796)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270644"
     CDS             1..944
                     /gene="LOC117605469"
                     /coded_by="XM_034326858.2:397..3231"
                     /db_xref="GeneID:117605469"
ORIGIN      
        1 mvksfgffar raipachhlf lflalaattt avdlsqciap lgmesgaipd aditasstfd
       61 tgnvgpqlar lkvenlggaw cpknqitqea rewleidlht vhlitatatq grfgnglgve
      121 yaeaylleyw rprlgkwvry rdikgkeviq gntntylesk helepplwas kirflpyshh
      181 trtvcmrvel ygcywsdgvv sysmpqgdkr gngweffdat ydghwdgelr rglgqltdgr
      241 tgpdnfkmgy ydndrnqgwv gwrndtrgqp veikfefdkv refsaihiyc nnqftkdvqv
      301 fshvdilfsi ggkyytgepi tytymedkif essrnitikl hhrvgkyvkl rlhfssrwil
      361 isevtfdsdv ahgnftpeea pttespiqsd vfvekngaae gelpvstakh ddptymavvi
      421 gvltavilll avaiflivsr hrqrkcfasp mtgkapshlg stcatvekga almaytledd
      481 eryaggslpt lprdlgnrll divklddyqe pyqalkyapy ysystvvmem kdmmlnnkgs
      541 ninhsavyan gavdtsydya vpelgtvpll nqdgtgraaa vstgttdqds ifsktssrss
      601 ktenkksptq qevlsalkrr leqtsvplfp rhrlrmlskl aegafgtvyv aeaegipeyg
      661 stttvgkrlv avkfllpeas ekekldfqrd vrilaaledr niarvlgacc reepycvvme
      721 ylehgdlcqf lkthitadda hsmpigvktl sfncliymaa qiasgmryle nlnfvhrdla
      781 trnclvgkay hikisdfgtd nelyacdyyk vdgtvplpir wmawesiflg kyttksdvwa
      841 favtlweiln lgrrvpyehl sdeevvqslr rlhratdcnd tggenncken ndnlfdylpq
      901 piacskdiyd lmldcwrree terptfreis mflqrknlgy apts
//
LOCUS       XP_034182761             631 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 [Osmia lignaria lignaria].
ACCESSION   XP_034182761
VERSION     XP_034182761.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326870.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="discoidin domain-containing receptor 2"
                     /calculated_mol_wt=71712
     Region          31..183
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(72,103,111)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     CDS             1..631
                     /gene="LOC117605478"
                     /coded_by="XM_034326870.2:538..2433"
                     /db_xref="GeneID:117605478"
ORIGIN      
        1 mdylqtclif yllilclsgg rsidisqcia plgmesgvip daditasssf dsgnvgphhg
       61 rlrqeshgga wcpkqqitte prewleidlh tvhmitatgt qgrfgngqgv eyseaymley
      121 wrpklgkwvr yrdvrgeevi kgntntyles kheleppmwa skvrflpysy hrrtvcmrve
      181 lygcpwndgi vsysmpqgdk rgnwelfdat ydgywdgqll rgmgqltdgk igpdnfkmgy
      241 ydydrgqgwv gwrndtrsgh pleikfefdh vrefsavhiy cnnqftkdvq vfseasimfs
      301 vggryytgdp ivysymedri fehsrnisik lhhrigkfvk lrfsfasrwi miseitfdsd
      361 vahgnftpes pptteaprqr drisardnpl qaevpvvkqd dptymaviig vltavillla
      421 vaiflivtrh rqrknfaspl stksaipsgn hqhlspesay gttekdpslm tyrveelddr
      481 yagtklttlp rdlndrllgd vrldeyqepf heskyrepph aayygystvv idnkdlhdnv
      541 eqsdatydya vpmpvpsvss dqdsvfskss srgsakaclq sffppppppm sappprgssn
      601 ltysnppspe pvcererrgs krrehslhry a
//
LOCUS       XP_076546331             631 aa            linear   INV 03-SEP-2025
DEFINITION  discoidin domain-containing receptor 2 [Osmia lignaria lignaria].
ACCESSION   XP_076546331
VERSION     XP_076546331.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690216.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="discoidin domain-containing receptor 2"
                     /calculated_mol_wt=71712
     Region          31..183
                     /region_name="FA58C"
                     /note="Coagulation factor 5/8 C-terminal domain, discoidin
                     domain; Cell surface-attached carbohydrate-binding domain,
                     present in eukaryotes and assumed to have horizontally
                     transferred to eubacterial genomes; cd00057"
                     /db_xref="CDD:238014"
     Site            order(72,103,111)
                     /site_type="other"
                     /note="sugar binding site [chemical binding]"
                     /db_xref="CDD:238014"
     CDS             1..631
                     /gene="LOC117605478"
                     /coded_by="XM_076690216.1:214..2109"
                     /db_xref="GeneID:117605478"
ORIGIN      
        1 mdylqtclif yllilclsgg rsidisqcia plgmesgvip daditasssf dsgnvgphhg
       61 rlrqeshgga wcpkqqitte prewleidlh tvhmitatgt qgrfgngqgv eyseaymley
      121 wrpklgkwvr yrdvrgeevi kgntntyles kheleppmwa skvrflpysy hrrtvcmrve
      181 lygcpwndgi vsysmpqgdk rgnwelfdat ydgywdgqll rgmgqltdgk igpdnfkmgy
      241 ydydrgqgwv gwrndtrsgh pleikfefdh vrefsavhiy cnnqftkdvq vfseasimfs
      301 vggryytgdp ivysymedri fehsrnisik lhhrigkfvk lrfsfasrwi miseitfdsd
      361 vahgnftpes pptteaprqr drisardnpl qaevpvvkqd dptymaviig vltavillla
      421 vaiflivtrh rqrknfaspl stksaipsgn hqhlspesay gttekdpslm tyrveelddr
      481 yagtklttlp rdlndrllgd vrldeyqepf heskyrepph aayygystvv idnkdlhdnv
      541 eqsdatydya vpmpvpsvss dqdsvfskss srgsakaclq sffppppppm sappprgssn
      601 ltysnppspe pvcererrgs krrehslhry a
//
LOCUS       XP_034179602             262 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604039 [Osmia lignaria lignaria].
ACCESSION   XP_034179602
VERSION     XP_034179602.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323711.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179602.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..262
                     /product="uncharacterized protein LOC117604039"
                     /calculated_mol_wt=30063
     CDS             1..262
                     /gene="LOC117604039"
                     /coded_by="XM_034323711.2:45..833"
                     /db_xref="GeneID:117604039"
ORIGIN      
        1 miltlhkvfn llelagkvtc twpedsstsk wtnvfrnirw ifalismivl lislafgvyd
       61 hrsnflllmk tmsemsgtsd vifdlilckm nstklqiliq rirkhletts eyenhviqgy
      121 ldrykefysv ivvayiatgi fftlaplfls qnlpadgwlp fstesfgiyc ivyilevycv
      181 wqaalifvin itivilfcft aarldilgtk lkevtcrell rsqkpfevst ggffpalsle
      241 yysqflttis syfmtmrtti kp
//
LOCUS       XP_034179608             282 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604050 [Osmia lignaria lignaria].
ACCESSION   XP_034179608
VERSION     XP_034179608.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323717.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179608.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 62% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="uncharacterized protein LOC117604050"
                     /calculated_mol_wt=32556
     Region          46..>208
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..282
                     /gene="LOC117604050"
                     /coded_by="XM_034323717.2:1..849"
                     /db_xref="GeneID:117604050"
ORIGIN      
        1 mfqlirfris rsavllnafl lflpltysly inyndhvgfs kaiclflavs qvllntfvci
       61 vqqdrfweli vemkkccnea ktyerqvyqt yvhkystfyg asaiwfylta siiaigtlfi
      121 pqpfptnaey pfpvdyqplk silflhqwff lyqcsahvci nmlcallmlf aaarfeilkv
      181 elrevkssne lidcvrkyyi arryakkvls asrfialntv mlcgtrqpst viiqfmslag
      241 talmevfmva wpadhlldlv sciltrfnif vpfarflsgq iv
//
LOCUS       XP_034196442             254 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611964 [Osmia lignaria lignaria].
ACCESSION   XP_034196442
VERSION     XP_034196442.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340551.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196442.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 20% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="uncharacterized protein LOC117611964"
                     /calculated_mol_wt=29012
     Region          <5..224
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..254
                     /gene="LOC117611964"
                     /coded_by="XM_034340551.2:50..814"
                     /db_xref="GeneID:117611964"
ORIGIN      
        1 myvdkysmfy gvsaiwfylt asiiaigtlf ipqplptnae ypfpvdyqpl ksilflhqsf
       61 lgfqcsahvc inmfcallml faaarfeilk velrevksfn elvdcvkkyy iarryakkvs
      121 sasrfialit vimcgtvlvf cgvnfierqp stviiqfmsl agtglmevfm vawpadhlld
      181 lsensmrgiy esrwylqsts mqknmlimll pqtpvrikil yiiptlsmny ycsfvsnvis
      241 lftvlratmq kddi
//
LOCUS       XP_034179620             354 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604055 [Osmia lignaria lignaria].
ACCESSION   XP_034179620
VERSION     XP_034179620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323729.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..354
                     /product="uncharacterized protein LOC117604055"
                     /calculated_mol_wt=40775
     Region          73..345
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..354
                     /gene="LOC117604055"
                     /coded_by="XM_034323729.2:68..1132"
                     /db_xref="GeneID:117604055"
ORIGIN      
        1 mlknitpeka iaftrysval tccwplssna tsfqlirfri srsavmlnaf lllppliysl
       61 ylnhndhvgf sksictiisv sqvllntfvc ivqydrfwel ivemnkycke akpyesqvyq
      121 tyiakyslfy tvstiwfylt asiiaigtff ipqpfptnae ypfhvdyqpl ksivflhqwf
      181 ffyqcsahvc inmlcallml faaarfeilk melrevkssn elincvkkhh ivrkyakevs
      241 sasrfialit vimcgmelvf cgvnfierqp stvvvqfmal sgtglievfm vawpadhlld
      301 msensmrgiy esqwylqsts mqkdmlimll rqapvgikiv yiipifslsy ycsv
//
LOCUS       XP_034191910             376 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609523 [Osmia lignaria lignaria].
ACCESSION   XP_034191910
VERSION     XP_034191910.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336019.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="uncharacterized protein LOC117609523"
                     /calculated_mol_wt=43235
     Region          70..346
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..376
                     /gene="LOC117609523"
                     /coded_by="XM_034336019.2:38..1168"
                     /db_xref="GeneID:117609523"
ORIGIN      
        1 mlknitpeka iaftrysval tccwpvssna psfqqiriri srsavllntf llflpliyss
       61 yinyvyndhv gfskaicili aavqvllntf vcivqydrfw elivemkkcc keakpyerqv
      121 yqtyvdkyst fytvftiwsy ltpstisigt lfipqpfptn aeypfpvdyq plktiiflhq
      181 wffllqcaah vcinllcall mwfaaarfei lkmelrevks snemidcvkk hhivrryake
      241 vssasrfial itvimcgmel vfcgvnfier qpstvivqfm alsgtgliev fmvawpadhl
      301 ldlsensmrg iyesqwylqs tsmqkdmlim llpqapvgik ivyiiptlsl nyycsfvsnv
      361 lslftvlrat iqeddi
//
LOCUS       XP_076546332             372 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604062 [Osmia lignaria lignaria].
ACCESSION   XP_076546332
VERSION     XP_076546332.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690217.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 28% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..372
                     /product="uncharacterized protein LOC117604062"
                     /calculated_mol_wt=42240
     Region          80..367
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..372
                     /gene="LOC117604062"
                     /coded_by="XM_076690217.1:13..1131"
                     /db_xref="GeneID:117604062"
ORIGIN      
        1 mfknvtpekt iaftrisvai scflplpsqa sklqvschki lrflvflnal cffpplvnal
       61 ftyndplnvs qsicfltgef qtfsntiicv ihhdrlrelm eamevclkna nvrenlvfqw
      121 yvdrfatfyg mstiwvyvtg tvtvlatlvt pdpfptnavy pfpvdyeplk tfifllqavt
      181 ileyvssawv nvlcallllf aaarfqtlym elrevknvde lincvkkyyi lkryanevvk
      241 amqfvtlftv mqsgvaivfc gitliqpqpf nvkvqylfvi atalmevfmc arpadhlldm
      301 senamqgiye srwydqstsv qkavsimllp qkpvairipc ivptlsldyf csfvtnvfsl
      361 fsvlraaisq dv
//
LOCUS       XP_034179971             130 aa            linear   INV 03-SEP-2025
DEFINITION  cuticle protein 7-like, partial [Osmia lignaria lignaria].
ACCESSION   XP_034179971
VERSION     XP_034179971.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324080.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..130
                     /product="cuticle protein 7-like"
     Region          60..111
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..130
                     /gene="LOC117604211"
                     /coded_by="XM_034324080.1:<1..393"
                     /db_xref="GeneID:117604211"
ORIGIN      
        1 iqlvasvlll stmfdwkvng hghahsfqhf hgpvtgdgqe vswkdkhghh ehdyvahphy
       61 efsygvddhh tgdyhgqkeh rdgkevvgey tvkepggnvr tvkyhagkhg ffahvhnsdg
      121 ndhhggghhy
//
LOCUS       XP_034182012            1411 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605128 [Osmia lignaria lignaria].
ACCESSION   XP_034182012
VERSION     XP_034182012.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326121.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182012.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1411
                     /product="uncharacterized protein LOC117605128"
                     /calculated_mol_wt=158964
     Region          362..>580
                     /region_name="U2AF_lg"
                     /note="U2 snRNP auxilliary factor, large subunit, splicing
                     factor; TIGR01642"
                     /db_xref="CDD:273727"
     CDS             1..1411
                     /gene="LOC117605128"
                     /coded_by="XM_034326121.2:176..4411"
                     /db_xref="GeneID:117605128"
ORIGIN      
        1 mlihlvflls lswphvigna dlqdevvfdq iatasenvae svkkleqkef rdqlipsmid
       61 ttihyensng fvpmvapiak rpdnlsevts spkianvtwt nssvsrvddk kkdkrgnien
      121 dpafsteari flnfpslsnn rrsfeyqgsq nefellkvrn dtpmvanypd fyedpsssme
      181 mkedeaessr hrnkgkgqtv qdytgnygmv rnenkerssy edyysrnvpk ekekksqska
      241 nanyhqqdav sydghgqtat rkngpttffs rtsqqsnvpa nsnpknheda ydsvaatsqi
      301 skthqkfsrp vvvaesnynf dhqssrksns dlddyqtari idssssemsh rhtirnrdgr
      361 frsdedsrdt sddseyveyt erprrvqksr rrpssadnsk rlqkehrssi desgefenqg
      421 krhhssrsks hrqrvrgnsw fdedrhhdqd esyedsrydg rtsssetrhq sskfkpsnsw
      481 nqvspnleis hsngveigql ekpklivpvk vnlvpvanfd hataigssqg fdvsnavlqn
      541 ivsgnplstp spvmstaenv lsqdskirms tpvpdiivgq ngyqnsvqtn eqnkfsmnfk
      601 pqfisttvap vyavtpslqs isvqdiqgas tprptyvsas nqvqhpstnq vqgngpqlvv
      661 pqptlqtist llhtpvpnsd fniqvnphgm hgqnpidhgn lqlqavptvs tvtstdhsvp
      721 mtkfsllpae sqskktlfpn sntnfltsan fpigqndqrq vngnyylqns nnqqvksqlq
      781 gnlyqqvlrn vnvapktkty vqtthllpal lhpmptiati aastpqvlsd hqnvqgnvvl
      841 qaprdqnqqf lkiqntgadn qlstlrklps lvyqtidnmg shstssvntv nafpgitdta
      901 hlpyvgtrnv eivnpnikps pidttvlnsy etmhypatvl ttsipmfttt sfvtarplls
      961 stttesshvq glvnslteig sknhqstgdl kayqsqdrpv fnpinfvpnm dlmksqsaln
     1021 sklhaseplp qnlnlvplip ggnffkpsft sqgellvkpk lnsdlatyae qmfkeslkti
     1081 yntqkwnndr kaanqarqna sdsdiaklkn elqrlkasly dskrnkdqle ghqtetkvqt
     1141 aelpgkkpde flaaleqmfk nhhtepphvy ygnnkphryr rpeqdkynpg skgehfketn
     1201 hikdfmtppq pnpyrskghf hdkpgkkrpi sgptrhnshh nhhhgprpyp rhhfshktgg
     1261 vegsatnldp vyldgfsqrg nlhdsrqrvr pefrksspfd syssfstqlp ekkgyfngfn
     1321 qqkgsigkei nhpkthnllg llmknkqlpe grntsyfrdq dqlkqffege kqrlqqqfyd
     1381 dtlkdyiykm sdghggsdtr rslphlqksr v
//
LOCUS       XP_034180005             527 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604254 [Osmia lignaria lignaria].
ACCESSION   XP_034180005
VERSION     XP_034180005.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324114.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180005.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 49% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..527
                     /product="uncharacterized protein LOC117604254"
                     /calculated_mol_wt=60026
     CDS             1..527
                     /gene="LOC117604254"
                     /coded_by="XM_034324114.2:1..1584"
                     /db_xref="GeneID:117604254"
ORIGIN      
        1 mlillvaltv avldtavgae yqfhlrsrrg veaghnpqef rqsryknpnv qntrtedarp
       61 ssyksgkinp eesvnapysn lrqsesqsne spyldklftm tasykvasdk ikpskqisth
      121 nqnpgtegay gafpsfnvqv plykssglqs hapifqnnpy sdatnqkkqs lpvlqlrntq
      181 dlstyltnfq nqpyllnptd yrfsfvgkap kvnnvqlgsp flsplssfqs qvvpistavn
      241 spqfpqykga siqvyptvgg fpatayqplq tqpqlhfqqd tmqrvqpvga vpqsapaqei
      301 radveiidkh pttpplkrdd edgdeeggyt pdekeyrpns etdeeyddkp dryfkaprte
      361 gnfkpsmtfp fkqydekfgk ysdqnheeev dydsnekils nkyqskdtls kpyysrqeee
      421 dddgrgsyek qkaeessent yrpkyfdknf deefgapyrk esppqkprrd esedpsseen
      481 rtslkyykap hyyqdneghs sdnlgrvskv iheegfgyri skgnkrf
//
LOCUS       XP_034180021             304 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604257 [Osmia lignaria lignaria].
ACCESSION   XP_034180021
VERSION     XP_034180021.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324130.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180021.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 15% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="uncharacterized protein LOC117604257"
                     /calculated_mol_wt=32176
     CDS             1..304
                     /gene="LOC117604257"
                     /coded_by="XM_034324130.2:1..915"
                     /db_xref="GeneID:117604257"
ORIGIN      
        1 malinpviks tssshesksv yilvlraeaf siidniiqsc fkmktigmii ialawasiae
       61 skpqgkkekr gvallgpsgy dffgvapifs tapwtptnfg stswsssgap dvpltqvqvq
      121 athdiaiqtl rdsssgtpsi ayppdvlkai qqakdanrnv nlaqqrvaea kqaaviqqkl
      181 alakeasare aaarsqeiss naeaearasa kqlvaaqqrl aslkdavaaa qrvaaareaa
      241 aaaaiqrnaa etaaqlqkqd vdkqisqseq eakekdiiaa kenavanavq lsalqkpphh
      301 pwnr
//
LOCUS       XP_034182471             473 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605327 [Osmia lignaria lignaria].
ACCESSION   XP_034182471
VERSION     XP_034182471.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326580.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182471.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 10% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="uncharacterized protein LOC117605327"
                     /calculated_mol_wt=48696
     CDS             1..473
                     /gene="LOC117605327"
                     /coded_by="XM_034326580.2:55..1476"
                     /db_xref="GeneID:117605327"
ORIGIN      
        1 msinvlllcl iagcalgsie aggdggwqgv assggydsgh gmelghvsgg glydslssyg
       61 sgygsygggg ydhglhgghv qhhyapavpv sehievtkpi pipvvknvgv pvahpvaiav
      121 phpvavgvpq pypvhvpvpk pvaipvvkti aipvekkvpy pvekvipvpv ekhvpipvek
      181 hipvpvekpy pihvpvykhv fhrvllslvs lataaplivk qdqndyqqyq lhggfqpier
      241 sdheslgsss gggsyggeys sgggygggyg ggyggghggg dygggeyggg ygggygggdf
      301 ggghggglea ssghlegssd hlssdyssyg ggyeggdegg vsldgghhsv vqsvpvsehv
      361 evtkpipikv ikhigvpvph ivkiavphpv avgvpqsypv ahpvpklvpv qvvktvavpv
      421 ekkvpfpvek hipvpvekpi pitiekhvpv pvikpypiki pvyktiyhha kkh
//
LOCUS       XP_034196637             966 aa            linear   INV 03-SEP-2025
DEFINITION  chromosome segregation 1 [Osmia lignaria lignaria].
ACCESSION   XP_034196637
VERSION     XP_034196637.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340746.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..966
                     /product="chromosome segregation 1"
                     /calculated_mol_wt=109960
     Region          29..103
                     /region_name="IBN_N"
                     /note="Importin-beta N-terminal domain; smart00913"
                     /db_xref="CDD:197981"
     Region          157..527
                     /region_name="Cse1"
                     /note="pfam08506"
                     /db_xref="CDD:430038"
     Region          528..959
                     /region_name="CAS_CSE1"
                     /note="CAS/CSE protein, C-terminus; pfam03378"
                     /db_xref="CDD:367469"
     CDS             1..966
                     /gene="Cse1"
                     /coded_by="XM_034340746.2:132..3032"
                     /db_xref="GeneID:117612060"
ORIGIN      
        1 meltddnllt lseylkhtls pdvnerrpae kflesvevnq nypllllhlv dkseinitir
       61 iagavafkny vkrnwkveed svdrihaqdr etikklivnl mlhspdsiqk qlsdavsiig
      121 kydfpnkwpe lidqmvekfn tgdfhvingv lhtahslfkk yryefksqsl wteikfvldr
      181 fakpltdlfv atmnltqvha nnvealkviy sslvilskvf yslnfqdlpe ffednmavwm
      241 rnfhtllntd vpslqssdde epevieqlks qvcdniglya qkydeefqpy lpqfvtavwn
      301 lltstgqqpk ydtlvsnalq flatvadrgq yrylfvdpgt lscicekvii pnmkfresdn
      361 elfednpeey irrdiegsdv dtrrraacdl vkvlskyfea kimeifgayi qvmlqnyaek
      421 ptenwrskda aiylvtssas kaqtqkhgvt qsselvplpq famqhiepel vkpnvnefpv
      481 lkadaikfim tfrsvlprem ilgslpqlir hlsatnivvh tyaacaieri lamkgpdnly
      541 lvkgtdlspl tadllkglfa clnmpgseen eyvmkaimrs fgvlqeivvp fladllpklt
      601 eklaivsknp srpnfnhflf etfalsikiv ckthkvavss feealfpifq eilqqdvlef
      661 lpyvfqilal llelrtqdmp eaylalfpcl lssvlferha nihplnrllr afishgahhi
      721 vaqdktngll gvfqkliask andhegflll qsiiehfapn vlepyvkqif vllfqrlsss
      781 kttkfvkgli vffayyiiry gasnlvtiid qiqsqmfgmv iervlladlq kvsgdierkv
      841 aavgisnlli dcpamlerpy ntyyprllat lveffelpqd qttlpeddaf peiedapgyq
      901 vgysqllcak npskdpleav gdvrlhlaqg larlsprqll nildqipepn anhlrtylqt
      961 vgitva
//
LOCUS       XP_034181836             624 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysoneless cell cycle regulator isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181836
VERSION     XP_034181836.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325945.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="ecdysoneless cell cycle regulator isoform X1"
                     /calculated_mol_wt=71829
     Region          28..573
                     /region_name="SGT1"
                     /note="SGT1 protein; pfam07093"
                     /db_xref="CDD:462089"
     CDS             1..624
                     /gene="ecd"
                     /coded_by="XM_034325945.2:33..1907"
                     /db_xref="GeneID:117604904"
ORIGIN      
        1 myqedikmsk ikeddvlecf lytkicytqp petiteeill qeiekfnevi apyvknyiwh
       61 sdeltfhprt kqalllekli esssvseecn ilphiyvclh fdedisdewf tvflifkltq
      121 tfngliakit dsdgefllie aanalpswan petcencvfi ynkelhivre kyntlfdlls
      181 niqekpylsk asekvqdalr krisiypdei nkrhhkarvf lpekavlilr qeprfvslai
      241 rtichsdple rkvcramkyf ppeqrvmvni kmtrclyama nhcrytgdpr tgwnlppvss
      301 skynahalgi kiacglemlv aranedrrkt ekrtindpek qkvnervlnt yltrlessgy
      361 frnllrdsqe ykkfenvakd yylehlnsnn hitnvnksda ekvleaweni qstdielhaq
      421 dettlspads dswlnvdpeq leaflseqwt ndknkkyeqe slslkekvkt flsrtsdigg
      481 vqfledhsmd tdiihdpedn gkvefdadif dctlrgildl vvpenegefe gssegslgqd
      541 dedkrgemdk ymhlldlelq seigkdesfe tnknpdtved nlfqsikeea ggsgpvgniv
      601 ggpaqrlmhl qlqspttvpp dlqs
//
LOCUS       XP_034182665             617 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysoneless cell cycle regulator isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182665
VERSION     XP_034182665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326774.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..617
                     /product="ecdysoneless cell cycle regulator isoform X2"
                     /calculated_mol_wt=70921
     Region          21..566
                     /region_name="SGT1"
                     /note="SGT1 protein; pfam07093"
                     /db_xref="CDD:462089"
     CDS             1..617
                     /gene="ecd"
                     /coded_by="XM_034326774.2:207..2060"
                     /db_xref="GeneID:117604904"
ORIGIN      
        1 mskikeddvl ecflytkicy tqppetitee illqeiekfn eviapyvkny iwhsdeltfh
       61 prtkqallle kliesssvse ecnilphiyv clhfdedisd ewftvflifk ltqtfnglia
      121 kitdsdgefl lieaanalps wanpetcenc vfiynkelhi vrekyntlfd llsniqekpy
      181 lskasekvqd alrkrisiyp deinkrhhka rvflpekavl ilrqeprfvs lairtichsd
      241 plerkvcram kyfppeqrvm vnikmtrcly amanhcrytg dprtgwnlpp vssskynaha
      301 lgikiacgle mlvaranedr rktekrtind pekqkvnerv lntyltrles sgyfrnllrd
      361 sqeykkfenv akdyylehln snnhitnvnk sdaekvleaw eniqstdiel haqdettlsp
      421 adsdswlnvd peqleaflse qwtndknkky eqeslslkek vktflsrtsd iggvqfledh
      481 smdtdiihdp edngkvefda difdctlrgi ldlvvpeneg efegssegsl gqddedkrge
      541 mdkymhlldl elqseigkde sfetnknpdt vednlfqsik eeaggsgpvg nivggpaqrl
      601 mhlqlqsptt vppdlqs
//
LOCUS       XP_034184613             588 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1-2 homolog isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184613
VERSION     XP_034184613.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328722.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184613.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="facilitated trehalose transporter Tret1-2
                     homolog isoform X1"
                     /calculated_mol_wt=63302
     Region          111..549
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(119..120,123..124,127,162,212..213,215..217,220,240,
                     243..244,247,362,365..366,369..371,374,402,406,465..466,
                     470,474,490,493..494,497..498,501)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..588
                     /gene="LOC117606334"
                     /coded_by="XM_034328722.2:1..1767"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 msgkmdgssd avfffedgda ggkrnshgtt edgryedsng ppgrralsee srmlrcdtvd
       61 yvevtkvdni cediennntd dkgnifddrn ivtrfssnsk gvfaqclvsg avlllaaggg
      121 mpigysaill pqlaqnngtm hadrelgswi asvhslatpv gslmsgplld gigrrgslrl
      181 tavplctgwi imgfaqnipt lligrvvsgl avglmavpaq vllgemadpg lrgfltgstl
      241 afyclgivii yalgatlawn mvafcgtilp ilalsallli pesptwlvrq kkpeearkal
      301 lwlrggnakq vnteiailea raktdlarmd lnmslaeqas aaistildps vlkpltiinv
      361 fnllqlisgt yvivfyavdl iadiggnrin nylaavitgi vrllfssiss vlllkvgrrs
      421 lgmfsafgta faslnlagym lfkkesssad iyvigiclll yvgvntlglm tlpalmvael
      481 lpqrargigg gcnffffnlf ifivtkvfpm vcetvgiagi fiifgisail egafiyvalp
      541 etnnrtleei edyfqqsnll wvtrsterrk daafimnnfs tqrmqnct
//
LOCUS       XP_034184760             550 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1-2 homolog isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184760
VERSION     XP_034184760.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328869.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184760.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..550
                     /product="facilitated trehalose transporter Tret1-2
                     homolog isoform X2"
                     /calculated_mol_wt=59550
     Region          73..511
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(81..82,85..86,89,124,174..175,177..179,182,202,
                     205..206,209,324,327..328,331..333,336,364,368,427..428,
                     432,436,452,455..456,459..460,463)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..550
                     /gene="LOC117606334"
                     /coded_by="XM_034328869.2:1801..3453"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 meflyskrqn eesrmlrcdt vdyvevtkvd nicediennn tddkgnifdd rnivtrfssn
       61 skgvfaqclv sgavlllaag ggmpigysai llpqlaqnng tmhadrelgs wiasvhslat
      121 pvgslmsgpl ldgigrrgsl rltavplctg wiimgfaqni ptlligrvvs glavglmavp
      181 aqvllgemad pglrgfltgs tlafyclgiv iiyalgatla wnmvafcgti lpilalsall
      241 lipesptwlv rqkkpeeark allwlrggna kqvnteiail earaktdlar mdlnmslaeq
      301 asaaistild psvlkpltii nvfnllqlis gtyvivfyav dliadiggnr innylaavit
      361 givrllfssi ssvlllkvgr rslgmfsafg tafaslnlag ymlfkkesss adiyvigicl
      421 llyvgvntlg lmtlpalmva ellpqrargi gggcnffffn lfifivtkvf pmvcetvgia
      481 gifiifgisa ilegafiyva lpetnnrtle eiedyfqqsn llwvtrster rkdaafimnn
      541 fstqrmqnct
//
LOCUS       XP_034184940             550 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1-2 homolog isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184940
VERSION     XP_034184940.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329049.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184940.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..550
                     /product="facilitated trehalose transporter Tret1-2
                     homolog isoform X2"
                     /calculated_mol_wt=59550
     Region          73..511
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(81..82,85..86,89,124,174..175,177..179,182,202,
                     205..206,209,324,327..328,331..333,336,364,368,427..428,
                     432,436,452,455..456,459..460,463)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..550
                     /gene="LOC117606334"
                     /coded_by="XM_034329049.2:1712..3364"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 meflyskrqn eesrmlrcdt vdyvevtkvd nicediennn tddkgnifdd rnivtrfssn
       61 skgvfaqclv sgavlllaag ggmpigysai llpqlaqnng tmhadrelgs wiasvhslat
      121 pvgslmsgpl ldgigrrgsl rltavplctg wiimgfaqni ptlligrvvs glavglmavp
      181 aqvllgemad pglrgfltgs tlafyclgiv iiyalgatla wnmvafcgti lpilalsall
      241 lipesptwlv rqkkpeeark allwlrggna kqvnteiail earaktdlar mdlnmslaeq
      301 asaaistild psvlkpltii nvfnllqlis gtyvivfyav dliadiggnr innylaavit
      361 givrllfssi ssvlllkvgr rslgmfsafg tafaslnlag ymlfkkesss adiyvigicl
      421 llyvgvntlg lmtlpalmva ellpqrargi gggcnffffn lfifivtkvf pmvcetvgia
      481 gifiifgisa ilegafiyva lpetnnrtle eiedyfqqsn llwvtrster rkdaafimnn
      541 fstqrmqnct
//
LOCUS       XP_076546333             546 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1-2 homolog isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546333
VERSION     XP_076546333.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690218.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="facilitated trehalose transporter Tret1-2
                     homolog isoform X3"
                     /calculated_mol_wt=58859
     Region          69..507
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77..78,81..82,85,120,170..171,173..175,178,198,
                     201..202,205,320,323..324,327..329,332,360,364,423..424,
                     428,432,448,451..452,455..456,459)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..546
                     /gene="LOC117606334"
                     /coded_by="XM_076690218.1:2053..3693"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 masteseesr mlrcdtvdyv evtkvdnice diennntddk gnifddrniv trfssnskgv
       61 faqclvsgav lllaagggmp igysaillpq laqnngtmha drelgswias vhslatpvgs
      121 lmsgplldgi grrgslrlta vplctgwiim gfaqniptll igrvvsglav glmavpaqvl
      181 lgemadpglr gfltgstlaf yclgiviiya lgatlawnmv afcgtilpil alsalllipe
      241 sptwlvrqkk peearkallw lrggnakqvn teiaileara ktdlarmdln mslaeqasaa
      301 istildpsvl kpltiinvfn llqlisgtyv ivfyavdlia diggnrinny laavitgivr
      361 llfssissvl llkvgrrslg mfsafgtafa slnlagymlf kkesssadiy vigiclllyv
      421 gvntlglmtl palmvaellp qrargigggc nffffnlfif ivtkvfpmvc etvgiagifi
      481 ifgisaileg afiyvalpet nnrtleeied yfqqsnllwv trsterrkda afimnnfstq
      541 rmqnct
//
LOCUS       XP_076546334             546 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1-2 homolog isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546334
VERSION     XP_076546334.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690219.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="facilitated trehalose transporter Tret1-2
                     homolog isoform X3"
                     /calculated_mol_wt=58859
     Region          69..507
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77..78,81..82,85,120,170..171,173..175,178,198,
                     201..202,205,320,323..324,327..329,332,360,364,423..424,
                     428,432,448,451..452,455..456,459)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..546
                     /gene="LOC117606334"
                     /coded_by="XM_076690219.1:1931..3571"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 masteseesr mlrcdtvdyv evtkvdnice diennntddk gnifddrniv trfssnskgv
       61 faqclvsgav lllaagggmp igysaillpq laqnngtmha drelgswias vhslatpvgs
      121 lmsgplldgi grrgslrlta vplctgwiim gfaqniptll igrvvsglav glmavpaqvl
      181 lgemadpglr gfltgstlaf yclgiviiya lgatlawnmv afcgtilpil alsalllipe
      241 sptwlvrqkk peearkallw lrggnakqvn teiaileara ktdlarmdln mslaeqasaa
      301 istildpsvl kpltiinvfn llqlisgtyv ivfyavdlia diggnrinny laavitgivr
      361 llfssissvl llkvgrrslg mfsafgtafa slnlagymlf kkesssadiy vigiclllyv
      421 gvntlglmtl palmvaellp qrargigggc nffffnlfif ivtkvfpmvc etvgiagifi
      481 ifgisaileg afiyvalpet nnrtleeied yfqqsnllwv trsterrkda afimnnfstq
      541 rmqnct
//
LOCUS       XP_034185370             546 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1-2 homolog isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185370
VERSION     XP_034185370.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329479.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185370.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="facilitated trehalose transporter Tret1-2
                     homolog isoform X3"
                     /calculated_mol_wt=58859
     Region          69..507
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77..78,81..82,85,120,170..171,173..175,178,198,
                     201..202,205,320,323..324,327..329,332,360,364,423..424,
                     428,432,448,451..452,455..456,459)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..546
                     /gene="LOC117606334"
                     /coded_by="XM_034329479.2:875..2515"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 masteseesr mlrcdtvdyv evtkvdnice diennntddk gnifddrniv trfssnskgv
       61 faqclvsgav lllaagggmp igysaillpq laqnngtmha drelgswias vhslatpvgs
      121 lmsgplldgi grrgslrlta vplctgwiim gfaqniptll igrvvsglav glmavpaqvl
      181 lgemadpglr gfltgstlaf yclgiviiya lgatlawnmv afcgtilpil alsalllipe
      241 sptwlvrqkk peearkallw lrggnakqvn teiaileara ktdlarmdln mslaeqasaa
      301 istildpsvl kpltiinvfn llqlisgtyv ivfyavdlia diggnrinny laavitgivr
      361 llfssissvl llkvgrrslg mfsafgtafa slnlagymlf kkesssadiy vigiclllyv
      421 gvntlglmtl palmvaellp qrargigggc nffffnlfif ivtkvfpmvc etvgiagifi
      481 ifgisaileg afiyvalpet nnrtleeied yfqqsnllwv trsterrkda afimnnfstq
      541 rmqnct
//
LOCUS       XP_034185453             546 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1-2 homolog isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185453
VERSION     XP_034185453.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329562.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185453.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="facilitated trehalose transporter Tret1-2
                     homolog isoform X3"
                     /calculated_mol_wt=58859
     Region          69..507
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(77..78,81..82,85,120,170..171,173..175,178,198,
                     201..202,205,320,323..324,327..329,332,360,364,423..424,
                     428,432,448,451..452,455..456,459)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..546
                     /gene="LOC117606334"
                     /coded_by="XM_034329562.2:1158..2798"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 masteseesr mlrcdtvdyv evtkvdnice diennntddk gnifddrniv trfssnskgv
       61 faqclvsgav lllaagggmp igysaillpq laqnngtmha drelgswias vhslatpvgs
      121 lmsgplldgi grrgslrlta vplctgwiim gfaqniptll igrvvsglav glmavpaqvl
      181 lgemadpglr gfltgstlaf yclgiviiya lgatlawnmv afcgtilpil alsalllipe
      241 sptwlvrqkk peearkallw lrggnakqvn teiaileara ktdlarmdln mslaeqasaa
      301 istildpsvl kpltiinvfn llqlisgtyv ivfyavdlia diggnrinny laavitgivr
      361 llfssissvl llkvgrrslg mfsafgtafa slnlagymlf kkesssadiy vigiclllyv
      421 gvntlglmtl palmvaellp qrargigggc nffffnlfif ivtkvfpmvc etvgiagifi
      481 ifgisaileg afiyvalpet nnrtleeied yfqqsnllwv trsterrkda afimnnfstq
      541 rmqnct
//
LOCUS       XP_076546335             460 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546335
VERSION     XP_076546335.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690220.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="facilitated trehalose transporter Tret1 isoform
                     X4"
                     /calculated_mol_wt=49081
     Region          111..>444
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..460
                     /gene="LOC117606334"
                     /coded_by="XM_076690220.1:1..1383"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 msgkmdgssd avfffedgda ggkrnshgtt edgryedsng ppgrralsee srmlrcdtvd
       61 yvevtkvdni cediennntd dkgnifddrn ivtrfssnsk gvfaqclvsg avlllaaggg
      121 mpigysaill pqlaqnngtm hadrelgswi asvhslatpv gslmsgplld gigrrgslrl
      181 tavplctgwi imgfaqnipt lligrvvsgl avglmavpaq vllgemadpg lrgfltgstl
      241 afyclgivii yalgatlawn mvafcgtilp ilalsallli pesptwlvrq kkpeearkal
      301 lwlrggnakq vnteiailea raktdlarmd lnmslaeqas aaistildps vlkpltiinv
      361 fnllqlisgt yvivfyavdl iadiggnrin nylaavitgi vrllfssiss vlllkvgrrs
      421 lgmfsafgta faslnlagym lfkkesssad yatsydhrmr
//
LOCUS       XP_034185783             460 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185783
VERSION     XP_034185783.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329892.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185783.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="facilitated trehalose transporter Tret1 isoform
                     X4"
                     /calculated_mol_wt=49081
     Region          111..>444
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..460
                     /gene="LOC117606334"
                     /coded_by="XM_034329892.2:1..1383"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 msgkmdgssd avfffedgda ggkrnshgtt edgryedsng ppgrralsee srmlrcdtvd
       61 yvevtkvdni cediennntd dkgnifddrn ivtrfssnsk gvfaqclvsg avlllaaggg
      121 mpigysaill pqlaqnngtm hadrelgswi asvhslatpv gslmsgplld gigrrgslrl
      181 tavplctgwi imgfaqnipt lligrvvsgl avglmavpaq vllgemadpg lrgfltgstl
      241 afyclgivii yalgatlawn mvafcgtilp ilalsallli pesptwlvrq kkpeearkal
      301 lwlrggnakq vnteiailea raktdlarmd lnmslaeqas aaistildps vlkpltiinv
      361 fnllqlisgt yvivfyavdl iadiggnrin nylaavitgi vrllfssiss vlllkvgrrs
      421 lgmfsafgta faslnlagym lfkkesssad yatsydhrmr
//
LOCUS       XP_034185866             448 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606334 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185866
VERSION     XP_034185866.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329975.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185866.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..448
                     /product="uncharacterized protein LOC117606334 isoform X5"
                     /calculated_mol_wt=48315
     Region          111..409
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     CDS             1..448
                     /gene="LOC117606334"
                     /coded_by="XM_034329975.2:1..1347"
                     /db_xref="GeneID:117606334"
ORIGIN      
        1 msgkmdgssd avfffedgda ggkrnshgtt edgryedsng ppgrralsee srmlrcdtvd
       61 yvevtkvdni cediennntd dkgnifddrn ivtrfssnsk gvfaqclvsg avlllaaggg
      121 mpigysaill pqlaqnngtm hadrelgswi asvhslatpv gslmsgplld gigrrgslrl
      181 tavplctgwi imgfaqnipt lligrvvsgl avglmavpaq vllgemadpg lrgfltgstl
      241 afyclgivii yalgatlawn mvafcgtilp ilalsallli pesptwlvrq kkpeearkal
      301 lwlrggnakq iyvigiclll yvgvntlglm tlpalmvael lpqrargigg gcnffffnlf
      361 ifivtkvfpm vcetvgiagi fiifgisail egafiyvalp etnnrtleei edyfqqsnll
      421 wvtrsterrk daafimnnfs tqrmqnct
//
LOCUS       XP_034196451             473 aa            linear   INV 03-SEP-2025
DEFINITION  tether containing UBX domain for GLUT4 [Osmia lignaria lignaria].
ACCESSION   XP_034196451
VERSION     XP_034196451.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340560.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196451.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="tether containing UBX domain for GLUT4"
                     /calculated_mol_wt=53828
     Region          7..75
                     /region_name="Ubl_ASPSCR1_like"
                     /note="Ubiquitin-like (Ubl) domain found at the N-terminus
                     of mammalian ASPSCR1 (alveolar soft part sarcoma
                     chromosomal region candidate gene 1 protein),
                     Saccharomyces cerevisie Ubx4p, and similar proteins;
                     cd16105"
                     /db_xref="CDD:340522"
     Region          83..157
                     /region_name="UBX1_UBXN9"
                     /note="Ubiquitin regulatory domain X (UBX) 1 found in UBX
                     domain protein 9 (UBXN9, UBXD9, or ASPSCR1) and similar
                     proteins; cd17075"
                     /db_xref="CDD:340595"
     Region          348..419
                     /region_name="UBX2_UBXN9"
                     /note="Ubiquitin regulatory domain X (UBX) 2 found in UBX
                     domain protein 9 (UBXN9, UBXD9, or ASPSCR1) and similar
                     proteins; cd16118"
                     /db_xref="CDD:340535"
     Site            order(349,351,353..354,357..358,360,392..393,395,412..415,
                     417,419)
                     /site_type="other"
                     /note="putative UBX-p97 interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:340535"
     CDS             1..473
                     /gene="LOC117611988"
                     /coded_by="XM_034340560.2:202..1623"
                     /db_xref="GeneID:117611988"
ORIGIN      
        1 matnknvivl apngrrqnvk vtanttilqm leevcqkqgy nvddydvkhf nqvldpnail
       61 rftglpnnaq lemipctktr lnstvtigvq lesgerlmae fmpnvtliev lqhldlnedl
      121 ekvaltymhc eiygvdtlkk ttlkslglnn gravlrlihr hprksndavs tksesvkgie
      181 nsnsdnkata snkavspiip lqkeeqrprk leicpqhver keqtiirtde thkvfkgeeg
      241 ksvismkkds cesspqtcyk reitrlpdie flgernalvf nqaemkpisr delpddfydl
      301 tvddakillr dvkryrkele eapllttvqr qlnqekrtln qlnkynytii rvhfadqfil
      361 qglfqpketv qmikdfikny ladpnsdfvi fttppkcild vntslinenl vpcanvyysg
      421 psalktdvkq kvtdpkkvkr qvaeirmami nqgdqisrnd vkinstnqge int
//
LOCUS       XP_034185165             409 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606422 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185165
VERSION     XP_034185165.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329274.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="uncharacterized protein LOC117606422 isoform X1"
                     /calculated_mol_wt=47005
     Region          38..91
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..409
                     /gene="LOC117606422"
                     /coded_by="XM_034329274.2:549..1778"
                     /db_xref="GeneID:117606422"
ORIGIN      
        1 mrrlnwvlei vlqciifalc iqseisnvvi pvktdfscln ktagfyadtg asckiyhtcd
       61 eygnkftyhc peetafrqda licdhahlvr chghtiynsk yieeknknss lnvspikdlv
      121 eksqnvfhvt qslktinkvr ygftistkey fenmnkpqva epnitnfhit nntsftatlp
      181 insfmkttlq hvssanntqe rgsikndnfs iqkqffnswd klnekdrtnq kdylnrktnd
      241 asydfvkfsv dvfpgndqnq ednaknyqti knitnlsklp ftnhrnypys etlksvqknt
      301 qisattnsik pehtlvvtte lpvyaltlsl kplipseley dpyypklsts tesyytpahs
      361 kelsymkdpv qatwssihfd lppilpdlns ledivdrrkl fyipritsn
//
LOCUS       XP_034185993             399 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606422 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185993
VERSION     XP_034185993.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330102.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="uncharacterized protein LOC117606422 isoform X2"
                     /calculated_mol_wt=45725
     Region          28..81
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..399
                     /gene="LOC117606422"
                     /coded_by="XM_034330102.2:139..1338"
                     /db_xref="GeneID:117606422"
ORIGIN      
        1 mlprpglwes pdfglpnvvi pvktdfscln ktagfyadtg asckiyhtcd eygnkftyhc
       61 peetafrqda licdhahlvr chghtiynsk yieeknknss lnvspikdlv eksqnvfhvt
      121 qslktinkvr ygftistkey fenmnkpqva epnitnfhit nntsftatlp insfmkttlq
      181 hvssanntqe rgsikndnfs iqkqffnswd klnekdrtnq kdylnrktnd asydfvkfsv
      241 dvfpgndqnq ednaknyqti knitnlsklp ftnhrnypys etlksvqknt qisattnsik
      301 pehtlvvtte lpvyaltlsl kplipseley dpyypklsts tesyytpahs kelsymkdpv
      361 qatwssihfd lppilpdlns ledivdrrkl fyipritsn
//
LOCUS       XP_076546336             203 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305688 [Osmia lignaria lignaria].
ACCESSION   XP_076546336
VERSION     XP_076546336.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690221.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..203
                     /product="uncharacterized protein LOC143305688"
                     /calculated_mol_wt=22945
     CDS             1..203
                     /gene="LOC143305688"
                     /coded_by="XM_076690221.1:1..612"
                     /db_xref="GeneID:143305688"
ORIGIN      
        1 mlanaiqtls tvmlsqsqsl ttlqgslnad rqgqnqlism asvhdtiref hgnegpesar
       61 awindlkntk tlhswseevt lsvakvrlrn gawkwlmnkg etvqsfqnfe dafvsaftys
      121 rtrseklkim sarvqkpnei lqdyvldkiw lckgldfsvg eirdpglwsr elshyvlgre
      181 yastdamlqd lvkvvtirqt ahq
//
LOCUS       XP_034180229             172 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604366 [Osmia lignaria lignaria].
ACCESSION   XP_034180229
VERSION     XP_034180229.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..172
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..172
                     /product="uncharacterized protein LOC117604366"
                     /calculated_mol_wt=18362
     CDS             1..172
                     /gene="LOC117604366"
                     /coded_by="XM_034324338.2:1..519"
                     /db_xref="GeneID:117604366"
ORIGIN      
        1 meklivvlai iefcwaselg wgghgltlge idvghegggf sldelggseh eiglgghggg
       61 gggghfipvv keigvpipkk vpfivpslqv qsipqsypvp vvvqkpvpyp vekqvftkve
      121 kkvptpveki ipvkvekhvp ftvvkhvpvp vpkpipikip vyktiihrhk gh
//
LOCUS       XP_034179672            1472 aa            linear   INV 03-SEP-2025
DEFINITION  thioester-containing protein 1 allele R1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179672
VERSION     XP_034179672.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323781.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179672.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1472
                     /product="thioester-containing protein 1 allele R1 isoform
                     X1"
                     /calculated_mol_wt=165672
     Region          <96..1282
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          926..1217
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(940,942..943,946,986,996,999,1051,1053..1054,1201)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            940..943
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1040,1044,1046,1054)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1323..1415
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1472
                     /gene="LOC117604080"
                     /coded_by="XM_034323781.2:220..4638"
                     /db_xref="GeneID:117604080"
ORIGIN      
        1 mdwqwfhifl sylivttvae syysiiapkv irpdseyhva vsttgvstts qiyieingvl
       61 dngevfnvsq vtniqpystr ilqfeigdii pgkyllsarg lsgiefsnsk pieyihkshs
      121 vfiqtdraiy kpgnkvmfrc illnsrlrps ldrlsdiyim dgkgnrikqw fhppithgif
      181 ngeielsesp vlgvwkiisn igdqtfekef evaeyvlpnf evtinapnhv tfkeskitat
      241 vyakytygkp vkgeatvtay pdifsgviqp ifqqpvrkvl pidgkvvvdf dihselrltd
      301 eferpvmidv tveesltgrt qntsmqitlh khkykmdlik tseyykpglk ytayikltyh
      361 dgtpvrddtn pvvimygyny nqsaytnitr mldkngmiql dfypsktkdn tslslnieaq
      421 ylnlhewfps tnqamslsne yiqavlktek ptvnreieie vnstsplkyi syeifgrgdi
      481 ldagsiyvqn kyttsfsfva tyvmaptahv ivyyigndge iiadaldvel edvlqnfvdi
      541 riapeevapg envnliitsk pnsyigllgv dqrsillksg ndisyeqiyr elksydlvqd
      601 spyadsvfdr flwspgsata ndvfresgai imtngyvhen fpirlpgile gritgsshga
      661 stlrpdlgpp vthklatrpp lagpyafsri pppvwnkprv flmhdilntw lftnfssgyd
      721 grnelkrnvp dsitswvlta fsvndvhglg liqeprklkv frpffismdl pysvirgeiv
      781 aiqivvfnym nkdvvaevll tnegqfdfae msnevhdvpk lelyrkkkin vkansgstvs
      841 fmiiprelgy ivikttansv lagdsverkl lvkaegetqy inkatfldlr stssmstnlt
      901 vdipknavpg sehveisavg dilgpsilnl anliklpsgc geqnmlnfvp nimilnylks
      961 tnqltqavqg kalrymeigy qreltyrhdd gsfsafgmsd sngstwltaf vakafkqaae
     1021 yipvedriid ealqwlsnnq apngsfpevg rvshrdmqgg aakglaltaf tliaflentd
     1081 svgkyrntim kgieyilrni nelddvyals ictyvinlak hpyenaafnl leskamtked
     1141 lkwwskpipn ndknpwyslp rpvdiemtsy alityirrnl itdsvpvmkw lvrqrnaegg
     1201 fastqdtvig iqalamlgeq latknntisv tftyegggqs hmninpdnsm ilqkqilprk
     1261 trlvnitatg sgfalvqvsf qynlnvtgaw plftldpqvd knsnanhlql sicsgfvptk
     1321 eanesnmvvm evnlpsgftv drdslpslev sqnvkrvetk ngdtvvilyf demnryeycp
     1381 tvsafrthkv akqkpvpvsi ydyydssrra rvfyeprkat lcdicdeedc edlcvsqpgk
     1441 qkdsqsgasy lctciylqsl falfyityle yl
//
LOCUS       XP_034179683            1472 aa            linear   INV 03-SEP-2025
DEFINITION  thioester-containing protein 1 allele R1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179683
VERSION     XP_034179683.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323792.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179683.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1472
                     /product="thioester-containing protein 1 allele R1 isoform
                     X1"
                     /calculated_mol_wt=165672
     Region          <96..1282
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          926..1217
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(940,942..943,946,986,996,999,1051,1053..1054,1201)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            940..943
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1040,1044,1046,1054)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1323..1415
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1472
                     /gene="LOC117604080"
                     /coded_by="XM_034323792.2:321..4739"
                     /db_xref="GeneID:117604080"
ORIGIN      
        1 mdwqwfhifl sylivttvae syysiiapkv irpdseyhva vsttgvstts qiyieingvl
       61 dngevfnvsq vtniqpystr ilqfeigdii pgkyllsarg lsgiefsnsk pieyihkshs
      121 vfiqtdraiy kpgnkvmfrc illnsrlrps ldrlsdiyim dgkgnrikqw fhppithgif
      181 ngeielsesp vlgvwkiisn igdqtfekef evaeyvlpnf evtinapnhv tfkeskitat
      241 vyakytygkp vkgeatvtay pdifsgviqp ifqqpvrkvl pidgkvvvdf dihselrltd
      301 eferpvmidv tveesltgrt qntsmqitlh khkykmdlik tseyykpglk ytayikltyh
      361 dgtpvrddtn pvvimygyny nqsaytnitr mldkngmiql dfypsktkdn tslslnieaq
      421 ylnlhewfps tnqamslsne yiqavlktek ptvnreieie vnstsplkyi syeifgrgdi
      481 ldagsiyvqn kyttsfsfva tyvmaptahv ivyyigndge iiadaldvel edvlqnfvdi
      541 riapeevapg envnliitsk pnsyigllgv dqrsillksg ndisyeqiyr elksydlvqd
      601 spyadsvfdr flwspgsata ndvfresgai imtngyvhen fpirlpgile gritgsshga
      661 stlrpdlgpp vthklatrpp lagpyafsri pppvwnkprv flmhdilntw lftnfssgyd
      721 grnelkrnvp dsitswvlta fsvndvhglg liqeprklkv frpffismdl pysvirgeiv
      781 aiqivvfnym nkdvvaevll tnegqfdfae msnevhdvpk lelyrkkkin vkansgstvs
      841 fmiiprelgy ivikttansv lagdsverkl lvkaegetqy inkatfldlr stssmstnlt
      901 vdipknavpg sehveisavg dilgpsilnl anliklpsgc geqnmlnfvp nimilnylks
      961 tnqltqavqg kalrymeigy qreltyrhdd gsfsafgmsd sngstwltaf vakafkqaae
     1021 yipvedriid ealqwlsnnq apngsfpevg rvshrdmqgg aakglaltaf tliaflentd
     1081 svgkyrntim kgieyilrni nelddvyals ictyvinlak hpyenaafnl leskamtked
     1141 lkwwskpipn ndknpwyslp rpvdiemtsy alityirrnl itdsvpvmkw lvrqrnaegg
     1201 fastqdtvig iqalamlgeq latknntisv tftyegggqs hmninpdnsm ilqkqilprk
     1261 trlvnitatg sgfalvqvsf qynlnvtgaw plftldpqvd knsnanhlql sicsgfvptk
     1321 eanesnmvvm evnlpsgftv drdslpslev sqnvkrvetk ngdtvvilyf demnryeycp
     1381 tvsafrthkv akqkpvpvsi ydyydssrra rvfyeprkat lcdicdeedc edlcvsqpgk
     1441 qkdsqsgasy lctciylqsl falfyityle yl
//
LOCUS       XP_034179692            1472 aa            linear   INV 03-SEP-2025
DEFINITION  thioester-containing protein 1 allele R1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179692
VERSION     XP_034179692.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323801.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179692.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1472
                     /product="thioester-containing protein 1 allele R1 isoform
                     X1"
                     /calculated_mol_wt=165672
     Region          <96..1282
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          926..1217
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(940,942..943,946,986,996,999,1051,1053..1054,1201)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            940..943
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1040,1044,1046,1054)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1323..1415
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1472
                     /gene="LOC117604080"
                     /coded_by="XM_034323801.2:89..4507"
                     /db_xref="GeneID:117604080"
ORIGIN      
        1 mdwqwfhifl sylivttvae syysiiapkv irpdseyhva vsttgvstts qiyieingvl
       61 dngevfnvsq vtniqpystr ilqfeigdii pgkyllsarg lsgiefsnsk pieyihkshs
      121 vfiqtdraiy kpgnkvmfrc illnsrlrps ldrlsdiyim dgkgnrikqw fhppithgif
      181 ngeielsesp vlgvwkiisn igdqtfekef evaeyvlpnf evtinapnhv tfkeskitat
      241 vyakytygkp vkgeatvtay pdifsgviqp ifqqpvrkvl pidgkvvvdf dihselrltd
      301 eferpvmidv tveesltgrt qntsmqitlh khkykmdlik tseyykpglk ytayikltyh
      361 dgtpvrddtn pvvimygyny nqsaytnitr mldkngmiql dfypsktkdn tslslnieaq
      421 ylnlhewfps tnqamslsne yiqavlktek ptvnreieie vnstsplkyi syeifgrgdi
      481 ldagsiyvqn kyttsfsfva tyvmaptahv ivyyigndge iiadaldvel edvlqnfvdi
      541 riapeevapg envnliitsk pnsyigllgv dqrsillksg ndisyeqiyr elksydlvqd
      601 spyadsvfdr flwspgsata ndvfresgai imtngyvhen fpirlpgile gritgsshga
      661 stlrpdlgpp vthklatrpp lagpyafsri pppvwnkprv flmhdilntw lftnfssgyd
      721 grnelkrnvp dsitswvlta fsvndvhglg liqeprklkv frpffismdl pysvirgeiv
      781 aiqivvfnym nkdvvaevll tnegqfdfae msnevhdvpk lelyrkkkin vkansgstvs
      841 fmiiprelgy ivikttansv lagdsverkl lvkaegetqy inkatfldlr stssmstnlt
      901 vdipknavpg sehveisavg dilgpsilnl anliklpsgc geqnmlnfvp nimilnylks
      961 tnqltqavqg kalrymeigy qreltyrhdd gsfsafgmsd sngstwltaf vakafkqaae
     1021 yipvedriid ealqwlsnnq apngsfpevg rvshrdmqgg aakglaltaf tliaflentd
     1081 svgkyrntim kgieyilrni nelddvyals ictyvinlak hpyenaafnl leskamtked
     1141 lkwwskpipn ndknpwyslp rpvdiemtsy alityirrnl itdsvpvmkw lvrqrnaegg
     1201 fastqdtvig iqalamlgeq latknntisv tftyegggqs hmninpdnsm ilqkqilprk
     1261 trlvnitatg sgfalvqvsf qynlnvtgaw plftldpqvd knsnanhlql sicsgfvptk
     1321 eanesnmvvm evnlpsgftv drdslpslev sqnvkrvetk ngdtvvilyf demnryeycp
     1381 tvsafrthkv akqkpvpvsi ydyydssrra rvfyeprkat lcdicdeedc edlcvsqpgk
     1441 qkdsqsgasy lctciylqsl falfyityle yl
//
LOCUS       XP_034179702            1472 aa            linear   INV 03-SEP-2025
DEFINITION  thioester-containing protein 1 allele R1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179702
VERSION     XP_034179702.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323811.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179702.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1472
                     /product="thioester-containing protein 1 allele R1 isoform
                     X1"
                     /calculated_mol_wt=165672
     Region          <96..1282
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          926..1217
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(940,942..943,946,986,996,999,1051,1053..1054,1201)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            940..943
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1040,1044,1046,1054)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1323..1415
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1472
                     /gene="LOC117604080"
                     /coded_by="XM_034323811.2:31..4449"
                     /db_xref="GeneID:117604080"
ORIGIN      
        1 mdwqwfhifl sylivttvae syysiiapkv irpdseyhva vsttgvstts qiyieingvl
       61 dngevfnvsq vtniqpystr ilqfeigdii pgkyllsarg lsgiefsnsk pieyihkshs
      121 vfiqtdraiy kpgnkvmfrc illnsrlrps ldrlsdiyim dgkgnrikqw fhppithgif
      181 ngeielsesp vlgvwkiisn igdqtfekef evaeyvlpnf evtinapnhv tfkeskitat
      241 vyakytygkp vkgeatvtay pdifsgviqp ifqqpvrkvl pidgkvvvdf dihselrltd
      301 eferpvmidv tveesltgrt qntsmqitlh khkykmdlik tseyykpglk ytayikltyh
      361 dgtpvrddtn pvvimygyny nqsaytnitr mldkngmiql dfypsktkdn tslslnieaq
      421 ylnlhewfps tnqamslsne yiqavlktek ptvnreieie vnstsplkyi syeifgrgdi
      481 ldagsiyvqn kyttsfsfva tyvmaptahv ivyyigndge iiadaldvel edvlqnfvdi
      541 riapeevapg envnliitsk pnsyigllgv dqrsillksg ndisyeqiyr elksydlvqd
      601 spyadsvfdr flwspgsata ndvfresgai imtngyvhen fpirlpgile gritgsshga
      661 stlrpdlgpp vthklatrpp lagpyafsri pppvwnkprv flmhdilntw lftnfssgyd
      721 grnelkrnvp dsitswvlta fsvndvhglg liqeprklkv frpffismdl pysvirgeiv
      781 aiqivvfnym nkdvvaevll tnegqfdfae msnevhdvpk lelyrkkkin vkansgstvs
      841 fmiiprelgy ivikttansv lagdsverkl lvkaegetqy inkatfldlr stssmstnlt
      901 vdipknavpg sehveisavg dilgpsilnl anliklpsgc geqnmlnfvp nimilnylks
      961 tnqltqavqg kalrymeigy qreltyrhdd gsfsafgmsd sngstwltaf vakafkqaae
     1021 yipvedriid ealqwlsnnq apngsfpevg rvshrdmqgg aakglaltaf tliaflentd
     1081 svgkyrntim kgieyilrni nelddvyals ictyvinlak hpyenaafnl leskamtked
     1141 lkwwskpipn ndknpwyslp rpvdiemtsy alityirrnl itdsvpvmkw lvrqrnaegg
     1201 fastqdtvig iqalamlgeq latknntisv tftyegggqs hmninpdnsm ilqkqilprk
     1261 trlvnitatg sgfalvqvsf qynlnvtgaw plftldpqvd knsnanhlql sicsgfvptk
     1321 eanesnmvvm evnlpsgftv drdslpslev sqnvkrvetk ngdtvvilyf demnryeycp
     1381 tvsafrthkv akqkpvpvsi ydyydssrra rvfyeprkat lcdicdeedc edlcvsqpgk
     1441 qkdsqsgasy lctciylqsl falfyityle yl
//
LOCUS       XP_034179718            1444 aa            linear   INV 03-SEP-2025
DEFINITION  thioester-containing protein 1 allele R1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179718
VERSION     XP_034179718.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323827.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179718.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1444
                     /product="thioester-containing protein 1 allele R1 isoform
                     X2"
                     /calculated_mol_wt=162720
     Region          <96..1254
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          898..1189
                     /region_name="A2M_2"
                     /note="Proteins similar to alpha2-macroglobulin (alpha
                     (2)-M). This group also contains the pregnancy zone
                     protein (PZP). Alpha(2)-M and PZP are broadly specific
                     proteinase inhibitors. Alpha (2)-M is a major carrier
                     protein in serum. The structural thioester...; cd02897"
                     /db_xref="CDD:239227"
     Site            order(912,914..915,918,958,968,971,1023,1025..1026,1173)
                     /site_type="other"
                     /note="surface patch"
                     /db_xref="CDD:239227"
     Site            912..915
                     /site_type="active"
                     /note="thioester region [active]"
                     /db_xref="CDD:239227"
     Site            order(1012,1016,1018,1026)
                     /site_type="other"
                     /note="specificity defining residues"
                     /db_xref="CDD:239227"
     Region          1295..1387
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1444
                     /gene="LOC117604080"
                     /coded_by="XM_034323827.2:220..4554"
                     /db_xref="GeneID:117604080"
ORIGIN      
        1 mdwqwfhifl sylivttvae syysiiapkv irpdseyhva vsttgvstts qiyieingvl
       61 dngevfnvsq vtniqpystr ilqfeigdii pgkyllsarg lsgiefsnsk pieyihkshs
      121 vfiqtdraiy kpgnkvmfrc illnsrlrps ldrlsdiyim dgkgnrikqw fhppithgif
      181 ngeielsesp vlgvwkiisn igdqtfekef evaeyvlpnf evtinapnhv tfkeskitat
      241 vyakytygkp vkgeatvtay pdifsgviqp ifqqpvrkvl pidgkvvvdf dihselrltd
      301 eferpvmidv tveesltgrt qntsmqitlh khkykmdlik tseyykpglk ytayikltyh
      361 dgtpvrddtn pvvimygyny nqsaytnitr mldkngmiql dfypsktkdn tslslnieaq
      421 ylnlhewfps tnqamslsne yiqavlktek ptvnreieie vnstsplkyi syeifgrgdi
      481 ldagsiyvqn kyttsfsfva tyvmaptahv ivyyigndge iiadaldvel edvlqnfvdi
      541 riapeevapg envnliitsk pnsyigllgv dqrsillksg ndisyeqiyr elksydlvqd
      601 spyadsvfdr flwspgsata ndvfresgai imtngyvhen fpilyyrsdl esaafptaig
      661 ssssialpse kmkirknfpe twlwqtldag ydgrnelkrn vpdsitswvl tafsvndvhg
      721 lgliqeprkl kvfrpffism dlpysvirge ivaiqivvfn ymnkdvvaev lltnegqfdf
      781 aemsnevhdv pklelyrkkk invkansgst vsfmiiprel gyivikttan svlagdsver
      841 kllvkaeget qyinkatfld lrstssmstn ltvdipknav pgsehveisa vgdilgpsil
      901 nlanliklps gcgeqnmlnf vpnimilnyl kstnqltqav qgkalrymei gyqreltyrh
      961 ddgsfsafgm sdsngstwlt afvakafkqa aeyipvedri idealqwlsn nqapngsfpe
     1021 vgrvshrdmq ggaakglalt aftliaflen tdsvgkyrnt imkgieyilr ninelddvya
     1081 lsictyvinl akhpyenaaf nlleskamtk edlkwwskpi pnndknpwys lprpvdiemt
     1141 syalityirr nlitdsvpvm kwlvrqrnae ggfastqdtv igiqalamlg eqlatknnti
     1201 svtftyeggg qshmninpdn smilqkqilp rktrlvnita tgsgfalvqv sfqynlnvtg
     1261 awplftldpq vdknsnanhl qlsicsgfvp tkeanesnmv vmevnlpsgf tvdrdslpsl
     1321 evsqnvkrve tkngdtvvil yfdemnryey cptvsafrth kvakqkpvpv siydyydssr
     1381 rarvfyeprk atlcdicdee dcedlcvsqp gkqkdsqsga sylctciylq slfalfyity
     1441 leyl
//
LOCUS       XP_034179732            1122 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase I subunit Rpl135 [Osmia lignaria lignaria].
ACCESSION   XP_034179732
VERSION     XP_034179732.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323841.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1122
                     /product="RNA polymerase I subunit Rpl135"
                     /calculated_mol_wt=127565
     Region          31..1117
                     /region_name="RNA_pol_B_RPB2"
                     /note="RNA polymerase beta subunit. RNA polymerases
                     catalyse the DNA dependent polymerization of RNA.
                     Prokaryotes contain a single RNA polymerase compared to
                     three in eukaryotes (not including mitochondrial. and
                     chloroplast polymerases). Each RNA polymerase...; cd00653"
                     /db_xref="CDD:238353"
     Site            order(98,764,796,798,802..804,806)
                     /site_type="other"
                     /note="RPB12 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Region          178..366
                     /region_name="RNA_pol_Rpb2_2"
                     /note="RNA polymerase Rpb2, domain 2; pfam04561"
                     /db_xref="CDD:398318"
     Site            order(480,482..484,486,489,494,654,672..674,676,739..742,
                     745,747..749,877,879,881,903,905,908,911,916,920,937,968,
                     976..978,980,993,996..998,1000..1001,1028,1030..1031,1033,
                     1035..1040,1042..1047,1051,1098,1102,1104,1106..1107,1109,
                     1111..1114,1116)
                     /site_type="other"
                     /note="RPB1 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Region          558..615
                     /region_name="RNA_pol_Rpa2_4"
                     /note="RNA polymerase I, Rpa2 specific domain; pfam06883"
                     /db_xref="CDD:462028"
     Site            order(712..713,734,736,757,760,886,899,926..927,929,964,
                     981)
                     /site_type="other"
                     /note="RPB10 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Site            order(745,759,885,970)
                     /site_type="other"
                     /note="RPB11 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     Site            order(759..760,764,859,861,886..888,891,893..894,969..976,
                     978)
                     /site_type="other"
                     /note="RPB3 interaction site [polypeptide binding]"
                     /db_xref="CDD:238353"
     CDS             1..1122
                     /gene="RpI135"
                     /coded_by="XM_034323841.2:74..3442"
                     /db_xref="GeneID:117604097"
ORIGIN      
        1 mliepkltnt skhfgkppnk qnpllqslgv phidsfnyml edglseavqd nplvyihlpn
       61 edkvalwldd vsihqpvvps gtigvknhki yptecrqrrc tykgkitvkv gwsingkvqe
      121 tlerdlgevp imvksnrchl hkmspkelva hgeheqewgg yfiikglerl irmllmtrrn
      181 ypiaikrsgw kargvqfsdl glllrcvrdd ntavnntlhy vtdgsaklmf thrkilyyvp
      241 lilmlkclid asdifiynal vagreddiwy kscisnmlra iheqdlhshe dckayigrmf
      301 rikffelpqd atdvdvcdfi ikhciaihln dpldkfyllv fmtkklfvla nnkcavegad
      361 avmmqecllg ghlylqvlke klynwlnilk tnilkrarsa gnryalnvqe mlnvmkhgnf
      421 ldsqmenfls tgnlrsstgl glmqntglti vaeninrmry mshfraihrg sffqemrtte
      481 arqllpdawg ficpvhtpdg apcgllnhlt mnciitkhpd pklkanvpii lmdlgmipls
      541 ivdnwqnsyv vmldgkligl iddsiisrvt dklrllkikg eevphtmeia lvpkknvpaq
      601 ypglylftna armmrpvmnl ackkieyigt feqiyleicv tpeeaykglt thqelsktaf
      661 lsnlaslipm pdynqsprnm yqcqmgkqtm gtpchtwqlq setklyrlqt patpffrpvh
      721 ydkidlddfa mgtnaivavi sytgydmeda miinkaaydr gfahgmiyks efvdlkdqrs
      781 yfarnpdkpe laekldtdgl pvpgtiiken dvyycyydad kstyvtgkyh gkedayvdnv
      841 klcgtlhshi prracitfri prnpsvgdkf asragqkgic sqkwpaedlp ftetglipdi
      901 ifnphgfpsr mtiammieim agksaaihgl vhdatsfrfn edetaveyfg kllerggyny
      961 ygterlysgi dgreltadif fgvvhyqrlr hmvsdkwqvr stgpvdvltr qpikgrrrgg
     1021 gvrfgemerd slishgcafl lqdrlfhcsd ktttlvcqkc gtllgpitem svttpglndk
     1081 trcrlcgdde svrdveipyi frylvtqlts cninvklsfv ek
//
LOCUS       XP_034179743             602 aa            linear   INV 03-SEP-2025
DEFINITION  HAUS augmin-like complex subunit 3 [Osmia lignaria lignaria].
ACCESSION   XP_034179743
VERSION     XP_034179743.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323852.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179743.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="HAUS augmin-like complex subunit 3"
                     /calculated_mol_wt=70513
     Region          38..>231
                     /region_name="HAUS-augmin3"
                     /note="HAUS augmin-like complex subunit 3; pfam14932"
                     /db_xref="CDD:464384"
     CDS             1..602
                     /gene="LOC117604104"
                     /coded_by="XM_034323852.2:256..2064"
                     /db_xref="GeneID:117604104"
ORIGIN      
        1 msisgkilyd kvrnlhsdls svitpeilek tcnepvvqpf lkwfcgnvns fniisnediq
       61 iknklqgtne wlegteldna leeatrscpd llkiisfdnl didglfsele iaknayvede
      121 nyintlqngi enlkkleskl dedlekeeai lsreyikadk aymecsaivk efdesnhkfs
      181 keikcllniy adaaenkgap llwtqmplel fiskielynh yldvyieqqf knihkeelkt
      241 dsnyvslmyn skekyidnek lqelslcnkt ltnakiklil akvqedsyia mlnhvqniyn
      301 sgnlkiprqs elrteisklt rkrdlleenv sllqehqlte ivqqftelei tkilkenadt
      361 kleetklsle rlknlrylar ehghaytdll cilmemqfhr lknvsefvad vyhylkmeyl
      421 lssarcesmq qeqtkyfylv nssskehnsf nqffismifn gdntcslnsa ldryneimde
      481 nknkkqlilk ahfnskihkl evwenevllq ymdeihkgct fsfepisyel etcygetsnn
      541 lqkiqadvrk irnqmkervk advsferekn ilwqrflvds etlkrihkkm ketankscfa
      601 qv
//
LOCUS       XP_034179759             547 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase subunit alpha blw, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034179759
VERSION     XP_034179759.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323868.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..547
                     /product="ATP synthase subunit alpha blw, mitochondrial"
                     /calculated_mol_wt=59269
     Region          41..546
                     /region_name="AtpA"
                     /note="FoF1-type ATP synthase, alpha subunit [Energy
                     production and conversion]; COG0056"
                     /db_xref="CDD:439826"
     CDS             1..547
                     /gene="blw"
                     /coded_by="XM_034323868.1:115..1758"
                     /db_xref="GeneID:117604108"
ORIGIN      
        1 mallslrlas siarqlpnst iqvkwplsva sckyhvscsr rsaeissile drilgsapkt
       61 nleetgrvls igdgiarvyg lkniqademv efssglkgma lnlepdnvgv vvfgndrhik
      121 egdivkrtga ivdvpvgdel lgrvvdalgn pidgkgplns klrfrigtka pgiiprtsvr
      181 epmqtgikav dslvpigrgq reliigdrqt gktalaidti inqkrfneag dekkklyciy
      241 vaigqkrstv aqivkrltds gamnytiivs atasdaaplq ylapysgcam geffrdngkh
      301 aliiyddlsk qavayrqmsl llrrppgrea ypgdvfylhs rlleraakmn eslgggslta
      361 lpvietqagd vsayiptnvi sitdgqifle telfykgirp ainvglsvsr vgsaaqtkam
      421 kqvagsmkle laqyrevaaf aqfgsdldaa tqqllnrgvr ltellkqgqy vpmaieeqva
      481 viycgvrgyl dkmeptkita fekeflahir atqqdllsti akentiseas daklkqivtd
      541 flasfsg
//
LOCUS       XP_034179765             516 aa            linear   INV 03-SEP-2025
DEFINITION  V-type proton ATPase subunit VhaSFD isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179765
VERSION     XP_034179765.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323874.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="V-type proton ATPase subunit VhaSFD isoform X1"
                     /calculated_mol_wt=59375
     Region          26..487
                     /region_name="VATPase_H"
                     /note="regulatory vacuolar ATP synthase subunit H
                     (Vma13p); activation component of the peripheral V1
                     complex of V-ATPase, a heteromultimeric enzyme which uses
                     ATP to actively transport protons into organelles and
                     extracellular compartments. The topology is...; cd00256"
                     /db_xref="CDD:238159"
     Site            order(95..110,112..121,128..129,131..136,139..160,
                     163..172,175..180,219..244,246..253,258..275,278..295,
                     302..315,324..348)
                     /site_type="other"
                     /note="armadillo-like superhelical repeats"
                     /db_xref="CDD:238159"
     Site            order(109,150,222,226)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     Site            order(289,330..332,337,371,375..376,380,385..386,388,393)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     CDS             1..516
                     /gene="VhaSFD"
                     /coded_by="XM_034323874.2:132..1682"
                     /db_xref="GeneID:117604113"
ORIGIN      
        1 mvdrahikei ipalpdekid mlaatsilqq qaadirnqri nwqsylqsqm iskedydfiv
       61 sfdtsdgnar eaklkenahq aaktflnllg hvskdqtiqy iltmiddmlq edrsrveifr
      121 ehssrkresv wgpflnllnr qdgfimnmts riiaklacws hdlmektdlq fyltwlkdql
      181 klsfealqdt nlhagieqni itdkeanelr elqnpnndyi qsvarclqmm lrideyrfaf
      241 vsvdgistll avltgrvnfq vqyqlifciw vltfnpllae kmnkfnvipi ladilsdsvk
      301 ekvtriilav frnliekved gqvakehcia mvqckvlkql silgqrkfdd editddiefl
      361 ndklqasvqd lssfdeyste vksgrlewsp vhksgkfwre nanrlnekny ellrilvhll
      421 etskdplvls vasfdigeyv rhyprgkhii eqlggkqrvm qllghedpnv ryeallavqk
      481 lmvhnweylg kqlekeqtgt sgssgnkpgt qvpaka
//
LOCUS       XP_034179773             516 aa            linear   INV 03-SEP-2025
DEFINITION  V-type proton ATPase subunit VhaSFD isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179773
VERSION     XP_034179773.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323882.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="V-type proton ATPase subunit VhaSFD isoform X1"
                     /calculated_mol_wt=59375
     Region          26..487
                     /region_name="VATPase_H"
                     /note="regulatory vacuolar ATP synthase subunit H
                     (Vma13p); activation component of the peripheral V1
                     complex of V-ATPase, a heteromultimeric enzyme which uses
                     ATP to actively transport protons into organelles and
                     extracellular compartments. The topology is...; cd00256"
                     /db_xref="CDD:238159"
     Site            order(95..110,112..121,128..129,131..136,139..160,
                     163..172,175..180,219..244,246..253,258..275,278..295,
                     302..315,324..348)
                     /site_type="other"
                     /note="armadillo-like superhelical repeats"
                     /db_xref="CDD:238159"
     Site            order(109,150,222,226)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     Site            order(289,330..332,337,371,375..376,380,385..386,388,393)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     CDS             1..516
                     /gene="VhaSFD"
                     /coded_by="XM_034323882.2:143..1693"
                     /db_xref="GeneID:117604113"
ORIGIN      
        1 mvdrahikei ipalpdekid mlaatsilqq qaadirnqri nwqsylqsqm iskedydfiv
       61 sfdtsdgnar eaklkenahq aaktflnllg hvskdqtiqy iltmiddmlq edrsrveifr
      121 ehssrkresv wgpflnllnr qdgfimnmts riiaklacws hdlmektdlq fyltwlkdql
      181 klsfealqdt nlhagieqni itdkeanelr elqnpnndyi qsvarclqmm lrideyrfaf
      241 vsvdgistll avltgrvnfq vqyqlifciw vltfnpllae kmnkfnvipi ladilsdsvk
      301 ekvtriilav frnliekved gqvakehcia mvqckvlkql silgqrkfdd editddiefl
      361 ndklqasvqd lssfdeyste vksgrlewsp vhksgkfwre nanrlnekny ellrilvhll
      421 etskdplvls vasfdigeyv rhyprgkhii eqlggkqrvm qllghedpnv ryeallavqk
      481 lmvhnweylg kqlekeqtgt sgssgnkpgt qvpaka
//
LOCUS       XP_076546337             516 aa            linear   INV 03-SEP-2025
DEFINITION  V-type proton ATPase subunit VhaSFD isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546337
VERSION     XP_076546337.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690222.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="V-type proton ATPase subunit VhaSFD isoform X1"
                     /calculated_mol_wt=59375
     Region          26..487
                     /region_name="VATPase_H"
                     /note="regulatory vacuolar ATP synthase subunit H
                     (Vma13p); activation component of the peripheral V1
                     complex of V-ATPase, a heteromultimeric enzyme which uses
                     ATP to actively transport protons into organelles and
                     extracellular compartments. The topology is...; cd00256"
                     /db_xref="CDD:238159"
     Site            order(95..110,112..121,128..129,131..136,139..160,
                     163..172,175..180,219..244,246..253,258..275,278..295,
                     302..315,324..348)
                     /site_type="other"
                     /note="armadillo-like superhelical repeats"
                     /db_xref="CDD:238159"
     Site            order(109,150,222,226)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     Site            order(289,330..332,337,371,375..376,380,385..386,388,393)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     CDS             1..516
                     /gene="VhaSFD"
                     /coded_by="XM_076690222.1:34..1584"
                     /db_xref="GeneID:117604113"
ORIGIN      
        1 mvdrahikei ipalpdekid mlaatsilqq qaadirnqri nwqsylqsqm iskedydfiv
       61 sfdtsdgnar eaklkenahq aaktflnllg hvskdqtiqy iltmiddmlq edrsrveifr
      121 ehssrkresv wgpflnllnr qdgfimnmts riiaklacws hdlmektdlq fyltwlkdql
      181 klsfealqdt nlhagieqni itdkeanelr elqnpnndyi qsvarclqmm lrideyrfaf
      241 vsvdgistll avltgrvnfq vqyqlifciw vltfnpllae kmnkfnvipi ladilsdsvk
      301 ekvtriilav frnliekved gqvakehcia mvqckvlkql silgqrkfdd editddiefl
      361 ndklqasvqd lssfdeyste vksgrlewsp vhksgkfwre nanrlnekny ellrilvhll
      421 etskdplvls vasfdigeyv rhyprgkhii eqlggkqrvm qllghedpnv ryeallavqk
      481 lmvhnweylg kqlekeqtgt sgssgnkpgt qvpaka
//
LOCUS       XP_034179781             484 aa            linear   INV 03-SEP-2025
DEFINITION  V-type proton ATPase subunit VhaSFD isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179781
VERSION     XP_034179781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="V-type proton ATPase subunit VhaSFD isoform X2"
                     /calculated_mol_wt=55728
     Region          26..455
                     /region_name="VATPase_H"
                     /note="regulatory vacuolar ATP synthase subunit H
                     (Vma13p); activation component of the peripheral V1
                     complex of V-ATPase, a heteromultimeric enzyme which uses
                     ATP to actively transport protons into organelles and
                     extracellular compartments. The topology is...; cd00256"
                     /db_xref="CDD:238159"
     Site            order(95..110,112..121,128..129,131..136,139..160,
                     163..172,175..180,187..212,214..221,226..243,246..263,
                     270..283,292..316)
                     /site_type="other"
                     /note="armadillo-like superhelical repeats"
                     /db_xref="CDD:238159"
     Site            order(109,150,190,194)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     Site            order(257,298..300,305,339,343..344,348,353..354,356,361)
                     /site_type="active"
                     /note="putative peptide binding site [active]"
                     /db_xref="CDD:238159"
     CDS             1..484
                     /gene="VhaSFD"
                     /coded_by="XM_034323890.2:130..1584"
                     /db_xref="GeneID:117604113"
ORIGIN      
        1 mvdrahikei ipalpdekid mlaatsilqq qaadirnqri nwqsylqsqm iskedydfiv
       61 sfdtsdgnar eaklkenahq aaktflnllg hvskdqtiqy iltmiddmlq edrsrveifr
      121 ehssrkresv wgpflnllnr qdgfimnmts riiaklacws hdlmektdlq fyltwlkdql
      181 klsnndyiqs varclqmmlr ideyrfafvs vdgistllav ltgrvnfqvq yqlifciwvl
      241 tfnpllaekm nkfnvipila dilsdsvkek vtriilavfr nliekvedgq vakehciamv
      301 qckvlkqlsi lgqrkfdded itddieflnd klqasvqdls sfdeystevk sgrlewspvh
      361 ksgkfwrena nrlneknyel lrilvhllet skdplvlsva sfdigeyvrh yprgkhiieq
      421 lggkqrvmql lghedpnvry eallavqklm vhnweylgkq lekeqtgtsg ssgnkpgtqv
      481 paka
//
LOCUS       XP_034179795             453 aa            linear   INV 03-SEP-2025
DEFINITION  elongation factor Tu 2-like [Osmia lignaria lignaria].
ACCESSION   XP_034179795
VERSION     XP_034179795.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323904.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="elongation factor Tu 2-like"
                     /calculated_mol_wt=50490
     Region          48..433
                     /region_name="TufA"
                     /note="Translation elongation factor EF-Tu, a GTPase
                     [Translation, ribosomal structure and biogenesis];
                     COG0050"
                     /db_xref="CDD:439820"
     CDS             1..453
                     /gene="LOC117604131"
                     /coded_by="XM_034323904.2:207..1568"
                     /db_xref="GeneID:117604131"
ORIGIN      
        1 mgfkniiqni rnkhcnlsvl lyrnnfpkfk qnlryfcdtt nspkegvcnv gtighvdhgk
       61 ttltsaitky lsevnkncky vtfdeidkap eekqrgitin iahvgyatsk rqyahtdcpg
      121 hadfiknmis gasqmdgail vvaaddgpmp qtkehlllvh qlgikhiviy inkadisdkd
      181 iielveiemr elldgygfds sktpiiigsa llalngdtse yglpsiksll daldnyvatp
      241 erdytspfil pidnvfsipg rgtvtvgtik qgvikkgika nlmgfdekmn tiisdiqifr
      301 ksvttasage nvgvllraik lnsvrrgmil cqtnflkltn hyeaqlylls pqeggrerpl
      361 kksgfsakvy sstwnvycrf dfllppenkm lmpgefatcr liftynmpmi dgqvftvree
      421 trtigtgkit rilneiplsq kifskvvikd fvn
//
LOCUS       XP_034179847             361 aa            linear   INV 03-SEP-2025
DEFINITION  cell cycle control protein 50A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179847
VERSION     XP_034179847.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323956.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..361
                     /product="cell cycle control protein 50A isoform X1"
                     /calculated_mol_wt=40560
     Region          56..329
                     /region_name="CDC50"
                     /note="LEM3 (ligand-effect modulator 3) family / CDC50
                     family; pfam03381"
                     /db_xref="CDD:460906"
     CDS             1..361
                     /gene="CDC50"
                     /coded_by="XM_034323956.2:150..1235"
                     /db_xref="GeneID:117604150"
ORIGIN      
        1 mtsvsegssl pktkkpsdsa fkqqrlpawq piltagtvlp tffvigiafi pvgvgllyfs
       61 dqvkeyiidy tdcnatnfhy angvpykcae viaknrsepc yceinftlps dfngkiymyy
      121 gltnfyqnhr ryvksrddnq llgklsdivs gdcepfaydg dkpiapcgai anslfsdkle
      181 lfsmkhnksv pllktgiawp sdknikfrnp dgdlreafkn fakpknwtky iydldkenes
      241 nngfqnedli vwmrtaalpt frklyrrvnh tengfteglv egnyrltvty sysvsafegr
      301 krmilsttsl lggknpflgi ayivvgcvcl llgitlliih ikcsksatem invspttpyq
      361 e
//
LOCUS       XP_034179858             349 aa            linear   INV 03-SEP-2025
DEFINITION  cell cycle control protein 50A isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179858
VERSION     XP_034179858.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323967.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..349
                     /product="cell cycle control protein 50A isoform X2"
                     /calculated_mol_wt=39269
     Region          56..329
                     /region_name="CDC50"
                     /note="LEM3 (ligand-effect modulator 3) family / CDC50
                     family; pfam03381"
                     /db_xref="CDD:460906"
     CDS             1..349
                     /gene="CDC50"
                     /coded_by="XM_034323967.2:153..1202"
                     /db_xref="GeneID:117604150"
ORIGIN      
        1 mtsvsegssl pktkkpsdsa fkqqrlpawq piltagtvlp tffvigiafi pvgvgllyfs
       61 dqvkeyiidy tdcnatnfhy angvpykcae viaknrsepc yceinftlps dfngkiymyy
      121 gltnfyqnhr ryvksrddnq llgklsdivs gdcepfaydg dkpiapcgai anslfsdkle
      181 lfsmkhnksv pllktgiawp sdknikfrnp dgdlreafkn fakpknwtky iydldkenes
      241 nngfqnedli vwmrtaalpt frklyrrvnh tengfteglv egnyrltvty sysvsafegr
      301 krmilsttsl lggknpflgi ayivvgcvcl llgitlliih ikcsksklm
//
LOCUS       XP_034179871             349 aa            linear   INV 03-SEP-2025
DEFINITION  guanine nucleotide-binding protein subunit beta-5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179871
VERSION     XP_034179871.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..349
                     /product="guanine nucleotide-binding protein subunit
                     beta-5"
                     /calculated_mol_wt=38290
     Region          53..349
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(60,78,82,88..89,101..102,120,124,130..131,147..148,
                     165,170,176..177,190,210,214,220..221,233..234,252,256,
                     262..263,275..276,295,300,306..307,319..320,338,342,
                     348..349)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          64..101
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          107..147
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          152..188
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          195..232
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          238..274
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          282..318
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          324..348
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..349
                     /gene="Gbeta5"
                     /coded_by="XM_034323980.2:30..1079"
                     /db_xref="GeneID:117604157"
ORIGIN      
        1 mdsvtesehi eklakeaesl kirlederqk lnditlasva drleainyin vkprrvlkgh
       61 qakvlcsdws pdkrhivsss qdgkmiiwda fttnkehalt mptiwvmaca ygpsgtlvac
      121 ggldnkvtvy plsqeddvst rkktvathts ymsccvfpns dqqiltgsgd stcalwdves
      181 gqllqsflgh ssdvmsidls psetgntfvs gscdkmvliw dmrtgqcvqs feghqsdvns
      241 vkfhpggdav atgsddatcr lfdlradrei avytkesiif ganavdfsvs grllfagynd
      301 ytvniwdtlk cqrvaflygh enrvsclrvs pdgtalstgs wdstlrvwa
//
LOCUS       XP_034179879             317 aa            linear   INV 03-SEP-2025
DEFINITION  receptor of activated protein kinase C 1 [Osmia lignaria lignaria].
ACCESSION   XP_034179879
VERSION     XP_034179879.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="receptor of activated protein kinase C 1"
                     /calculated_mol_wt=35563
     Region          7..311
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cd00200"
                     /db_xref="CDD:238121"
     Site            order(14,33,37,43..44,61..62,80,84,90..91,103..104,121,
                     126,132..133,147,166,171,177..178,190..191,209,213,
                     219..220,232,249,253,259..260,281..282,300,304,310..311)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          18..61
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          67..103
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          108..144
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          152..189
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          195..231
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          236..279
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          286..310
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..317
                     /gene="Rack1"
                     /coded_by="XM_034323988.2:120..1073"
                     /db_xref="GeneID:117604165"
ORIGIN      
        1 mtetlqlrgt lrghngwvtq iatnpkypdm ilsssrdktl ivwkltrdea nygipqkrly
       61 ghshyisdvv lssdgnyals gswdktlrlw dlaagrttrr fedhtkdvls vafsvdnrqi
      121 vsgsrdktik lwntlaecky tiqddghtdw vscvrfspnh snpiivsagw dklvkvwnlt
      181 ncklkinhsg htgylntvtv spdgslcasg gkdckamlwd lndgkhlhtl dhndiitalc
      241 fspnrywlca afgpwikiwd letkemveel kpevvsatsk aepphclsla wstdgqtlfa
      301 gysdntirvw qvsvssh
//
LOCUS       XP_034179892             277 aa            linear   INV 03-SEP-2025
DEFINITION  NFU1 iron-sulfur cluster scaffold homolog, mitochondrial [Osmia
            lignaria lignaria].
ACCESSION   XP_034179892
VERSION     XP_034179892.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324001.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179892.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..277
                     /product="NFU1 iron-sulfur cluster scaffold homolog,
                     mitochondrial"
                     /calculated_mol_wt=31381
     Region          67..155
                     /region_name="Nfu_N"
                     /note="Scaffold protein Nfu/NifU N terminal; smart00932"
                     /db_xref="CDD:214919"
     Region          73..248
                     /region_name="NifU"
                     /note="Fe-S cluster biogenesis protein NfuA,
                     4Fe-4S-binding domain [Posttranslational modification,
                     protein turnover, chaperones]; COG0694"
                     /db_xref="CDD:440458"
     CDS             1..277
                     /gene="LOC117604171"
                     /coded_by="XM_034324001.2:140..973"
                     /db_xref="GeneID:117604171"
ORIGIN      
        1 mmdkiltnal ymsrrltcry caafqfsktl kdctvrmytn ynnhmvkpcl ftsipklnvi
       61 qkrqmfiqtq dtpnpnslkf lpgvkvlesg qtkdfpnvsd aycsplakml friegvksif
      121 fgpdfitvtk vdedvewkll kpevfavimd ffasglpvln deqpatdtqi seddseivqm
      181 ikelldtrir ptvqedggdi vfvgfeegiv klkmqgsctg cpssvvtlrn gvqnmmqfyi
      241 pevlgviqve detdqiakae feqfetklkk setnekk
//
LOCUS       XP_034182944             145 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605557 [Osmia lignaria lignaria].
ACCESSION   XP_034182944
VERSION     XP_034182944.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327053.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..145
                     /product="uncharacterized protein LOC117605557"
                     /calculated_mol_wt=16447
     CDS             1..145
                     /gene="LOC117605557"
                     /coded_by="XM_034327053.2:1..438"
                     /db_xref="GeneID:117605557"
ORIGIN      
        1 mrifiltalv tmvcasyeed hggstyhets kpveipiykk yaipiphpvp vpvpqhikvp
       61 ipqpyqvevp vphpvpvevv khveipvekp epyvvekkvp yvvekpypvt vekhfavpip
      121 kpypvhvpvy khvfhhqskg hgwkh
//
LOCUS       XP_034184906             906 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606506 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184906
VERSION     XP_034184906.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329015.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184906.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..906
                     /product="uncharacterized protein LOC117606506 isoform X1"
                     /calculated_mol_wt=102328
     Region          47..98
                     /region_name="bHLH_PAS"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain family; cd11391"
                     /db_xref="CDD:381397"
     Site            order(47,50..51,54..55,57..58,62,83..84)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381397"
     Site            order(57,60..61,64..65,67..68,71..73,83..85,88..89,92,
                     95..96,98)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381397"
     Region          122..181
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          308..415
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     CDS             1..906
                     /gene="LOC117606506"
                     /coded_by="XM_034329015.2:1542..4262"
                     /db_xref="GeneID:117606506"
ORIGIN      
        1 mwreqeqihl hpqhpqqqil hrgiigtver thhrsttmdl pgpsnprasr nmaekqrrdn
       61 lntnisamat liptvaespr kmdkisilrl aaaflrmrya lgrgtldflp relgeldleq
      121 yivdnligtg gfflvltttg kivyvsrqvq ehlghaqadl lghslytfvh pkdhdelnkn
      181 ltpdemqgvv ssstsqitdg lndnsissed ssssnnerrp freqrrsfel rilhrtasrr
      241 ehtqyeclev sgmlrladac knpesnanrm rhrevsstsn divfvgvarv mmkrpiteis
      301 imdankdeyv trhlvdgrii ycdhrvsvva gylseevsgl safsfmhkdd frwtmivlrq
      361 mydrvetcgs scyrllsktg efiylrthgy lefdkdtqtv esfvcintlv seeegirlvk
      421 qmkkrfsatv sdttrtmiqc kddasfdlgt dsqqsnskss vedpsqleda ithlvsdlss
      481 svsedhmsds pvpneqyaka amvsqhlppa atqasklgik kidhylvvqg kgnrtpkqts
      541 ktnndkyekq rnprsigkti tihdvthiqd tkdpkisapl nevivannts versinvprd
      601 pgtshpnvcr nfnilspaav vnepksivss slhespcdik vehaidvfkr qqepiieyhe
      661 sgiadnsias daktshqysl kriysdedir tscnkkrhsn vtyvsdknde pqnvsyvdcr
      721 pfvteeyldi ptdfnqysni gsqslklaes pnstlnegiv dyqqvdssvp llppnpddef
      781 ihdlkddpll spslnanpdi imkifddlrh vpifensfne tkvqladnna inneikrkhl
      841 qlmnsislqe sqlnvlardl knpalqakrg sltplqaehn mqkqiletlq qdhhniqini
      901 khnigv
//
LOCUS       XP_034184917             888 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606506 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184917
VERSION     XP_034184917.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329026.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..888
                     /product="uncharacterized protein LOC117606506 isoform X2"
                     /calculated_mol_wt=99803
     Region          29..80
                     /region_name="bHLH_PAS"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain family; cd11391"
                     /db_xref="CDD:381397"
     Site            order(29,32..33,36..37,39..40,44,65..66)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381397"
     Site            order(39,42..43,46..47,49..50,53..55,65..67,70..71,74,
                     77..78,80)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381397"
     Region          104..163
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          290..397
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     CDS             1..888
                     /gene="LOC117606506"
                     /coded_by="XM_034329026.2:276..2942"
                     /db_xref="GeneID:117606506"
ORIGIN      
        1 mkddvdeddg galsstsvpe ttisygnpra srnmaekqrr dnlntnisam atliptvaes
       61 prkmdkisil rlaaaflrmr yalgrgtldf lprelgeldl eqyivdnlig tggfflvltt
      121 tgkivyvsrq vqehlghaqa dllghslytf vhpkdhdeln knltpdemqg vvssstsqit
      181 dglndnsiss edssssnner rpfreqrrsf elrilhrtas rrehtqyecl evsgmlrlad
      241 acknpesnan rmrhrevsst sndivfvgva rvmmkrpite isimdankde yvtrhlvdgr
      301 iiycdhrvsv vagylseevs glsafsfmhk ddfrwtmivl rqmydrvetc gsscyrllsk
      361 tgefiylrth gylefdkdtq tvesfvcint lvseeegirl vkqmkkrfsa tvsdttrtmi
      421 qckddasfdl gtdsqqsnsk ssvedpsqle daithlvsdl sssvsedhms dspvpneqya
      481 kaamvsqhlp paatqasklg ikkidhylvv qgkgnrtpkq tsktnndkye kqrnprsigk
      541 titihdvthi qdtkdpkisa plnevivann tsversinvp rdpgtshpnv crnfnilspa
      601 avvnepksiv ssslhespcd ikvehaidvf krqqepiiey hesgiadnsi asdaktshqy
      661 slkriysded irtscnkkrh snvtyvsdkn depqnvsyvd crpfvteeyl diptdfnqys
      721 nigsqslkla espnstlneg ivdyqqvdss vpllppnpdd efihdlkddp llspslnanp
      781 diimkifddl rhvpifensf netkvqladn nainneikrk hlqlmnsisl qesqlnvlar
      841 dlknpalqak rgsltplqae hnmqkqilet lqqdhhniqi nikhnigv
//
LOCUS       XP_034184941             542 aa            linear   INV 03-SEP-2025
DEFINITION  tryptophan hydroxylase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184941
VERSION     XP_034184941.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329050.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184941.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="tryptophan hydroxylase isoform X1"
                     /calculated_mol_wt=61314
     Region          23..478
                     /region_name="Trp_5_monoox"
                     /note="tryptophan 5-monooxygenase, tetrameric; TIGR01270"
                     /db_xref="CDD:130337"
     CDS             1..542
                     /gene="Trhn"
                     /coded_by="XM_034329050.2:3792..5420"
                     /db_xref="GeneID:117606514"
ORIGIN      
        1 msgsgkgllg vwlykrgehw aikegspihk ssdipmverq pnndsknsvv fslknqvggl
       61 aralqvfqdl gvnvvhiesr rsmrrgsefe ilvdvecdsk rmeqltrmls revaainlaq
      121 yeqtgniphg pmlsaaasfd fsevdmpwfp rkisdldqaq kvlmygseld adhpgfkdpi
      181 yrkrrvqfad iannykygqp iprvqytsee irtwgtvfre lhqlyqkhac keylenwpkl
      241 vkycgyredn ipqlqdvnaf lkrttgfqlr pvagylsprd flaglafrvf hctqyirhss
      301 dpfytpepdc chellghmpl lanpsfaqfs qelglaslga sdedinklat lyfftvefgl
      361 ckqdgvlrvy gagllssvae lkhavsapek tfrfepdvtc kqeciitafq nayyytdcfe
      421 eakekmrsfa nqiqrpfglr ynpytqsvev ltdaqkitav vselrgdlci vsnalrkihe
      481 qddtvdveri tslltqgiel pqdtsssdsd idkspnvedp qnpvegqnke yssdgnmisa
      541 ld
//
LOCUS       XP_076546338             546 aa            linear   INV 03-SEP-2025
DEFINITION  tryptophan hydroxylase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546338
VERSION     XP_076546338.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690223.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="tryptophan hydroxylase isoform X2"
                     /calculated_mol_wt=61709
     Region          23..482
                     /region_name="Trp_5_monoox"
                     /note="tryptophan 5-monooxygenase, tetrameric; TIGR01270"
                     /db_xref="CDD:130337"
     CDS             1..546
                     /gene="Trhn"
                     /coded_by="XM_076690223.1:3792..5432"
                     /db_xref="GeneID:117606514"
ORIGIN      
        1 msgsgkgllg vwlykrgehw aikegspihk ssdvrsfkis akckpnndsk nsvvfslknq
       61 vgglaralqv fqdlgvnvvh iesrrsmrrg sefeilvdve cdskrmeqlt rmlsrevaai
      121 nlaqyeqtgn iphgpmlsaa asfdfsevdm pwfprkisdl dqaqkvlmyg seldadhpgf
      181 kdpiyrkrrv qfadiannyk ygqpiprvqy tseeirtwgt vfrelhqlyq khackeylen
      241 wpklvkycgy rednipqlqd vnaflkrttg fqlrpvagyl sprdflagla frvfhctqyi
      301 rhssdpfytp epdcchellg hmpllanpsf aqfsqelgla slgasdedin klatlyfftv
      361 efglckqdgv lrvygaglls svaelkhavs apektfrfep dvtckqecii tafqnayyyt
      421 dcfeeakekm rsfanqiqrp fglrynpytq svevltdaqk itavvselrg dlcivsnalr
      481 kiheqddtvd veritslltq gielpqdtss sdsdidkspn vedpqnpveg qnkeyssdgn
      541 misald
//
LOCUS       XP_034184952             502 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein Aplip1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184952
VERSION     XP_034184952.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329061.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184952.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="JNK-interacting protein Aplip1 isoform X1"
                     /calculated_mol_wt=54722
     Region          290..344
                     /region_name="SH3_JIP1_like"
                     /note="Src homology 3 domain of JNK-interacting proteins 1
                     and 2, and similar domains; cd11801"
                     /db_xref="CDD:212735"
     Site            order(295..298,300..301,303..304,319,321..323,325,336,
                     338..341)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:212735"
     Site            order(295,297,300,304,322..323,338,340..341)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212735"
     Region          358..502
                     /region_name="PTB_JIP"
                     /note="JNK-interacting protein-like (JIP)
                     Phosphotyrosine-binding (PTB) domain; cd01212"
                     /db_xref="CDD:269923"
     Site            order(365,446,466)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269923"
     Site            order(435..440,453,475,479)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269923"
     CDS             1..502
                     /gene="Aplip1"
                     /coded_by="XM_034329061.2:53..1561"
                     /db_xref="GeneID:117606522"
ORIGIN      
        1 madtefeefr qyferlpqhl kaplsnytlv hdvmiddspt ssqgsdaevg rscdgvagds
       61 edeevvvihr hdnqsghtsg ddsedldhnc sivadsdfrl vtslfgglss rgrsagvggp
      121 gpgggsttvs gnggrdsvfs dvgdscssls swptpehvps nrpgsggter rrrrlpeipk
      181 tkksvgqmfm qssssladel naaagstgst rphlslrkch srlrhedssp dsermatdsg
      241 hstahspeng pksvspipcn tlqntdsasp sstpgsggvp ftqlelleat hrglhkfvpr
      301 hhdeidleig dpiyvqkead dlwcegvnlr tgrqgifpsa yavdmdysdf dpsapkvkre
      361 ryllgylgsv etlahkgtgv vcqavrrivg nsqespvsqs cilevsdqgl rmvdrskprk
      421 nqgpchdyfy slknvsfcaf hprdhrylgf itkhptlqrf achvfigqes trpvaeavgr
      481 afhrfytkfi etafpvediy ie
//
LOCUS       XP_034184961             498 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein Aplip1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184961
VERSION     XP_034184961.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329070.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184961.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="JNK-interacting protein Aplip1 isoform X2"
                     /calculated_mol_wt=54252
     Region          286..340
                     /region_name="SH3_JIP1_like"
                     /note="Src homology 3 domain of JNK-interacting proteins 1
                     and 2, and similar domains; cd11801"
                     /db_xref="CDD:212735"
     Site            order(291..294,296..297,299..300,315,317..319,321,332,
                     334..337)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:212735"
     Site            order(291,293,296,300,318..319,334,336..337)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212735"
     Region          354..498
                     /region_name="PTB_JIP"
                     /note="JNK-interacting protein-like (JIP)
                     Phosphotyrosine-binding (PTB) domain; cd01212"
                     /db_xref="CDD:269923"
     Site            order(361,442,462)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269923"
     Site            order(431..436,449,471,475)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269923"
     CDS             1..498
                     /gene="Aplip1"
                     /coded_by="XM_034329070.2:53..1549"
                     /db_xref="GeneID:117606522"
ORIGIN      
        1 madtefeefr qyferlpqhl kaplsnytlv hdvmiddspt ssqgsdaevg rscdgvagds
       61 edeevvvihr hdnqsghtsg ddsedldhnc sivadsdfsl fgglssrgrs agvggpgpgg
      121 gsttvsgngg rdsvfsdvgd scsslsswpt pehvpsnrpg sggterrrrr lpeipktkks
      181 vgqmfmqsss sladelnaaa gstgstrphl slrkchsrlr hedsspdser matdsghsta
      241 hspengpksv spipcntlqn tdsaspsstp gsggvpftql elleathrgl hkfvprhhde
      301 idleigdpiy vqkeaddlwc egvnlrtgrq gifpsayavd mdysdfdpsa pkvkreryll
      361 gylgsvetla hkgtgvvcqa vrrivgnsqe spvsqscile vsdqglrmvd rskprknqgp
      421 chdyfyslkn vsfcafhprd hrylgfitkh ptlqrfachv figqestrpv aeavgrafhr
      481 fytkfietaf pvediyie
//
LOCUS       XP_034184970             495 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein Aplip1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184970
VERSION     XP_034184970.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329079.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184970.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="JNK-interacting protein Aplip1 isoform X3"
                     /calculated_mol_wt=54060
     Region          283..337
                     /region_name="SH3_JIP1_like"
                     /note="Src homology 3 domain of JNK-interacting proteins 1
                     and 2, and similar domains; cd11801"
                     /db_xref="CDD:212735"
     Site            order(288..291,293..294,296..297,312,314..316,318,329,
                     331..334)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:212735"
     Site            order(288,290,293,297,315..316,331,333..334)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212735"
     Region          351..495
                     /region_name="PTB_JIP"
                     /note="JNK-interacting protein-like (JIP)
                     Phosphotyrosine-binding (PTB) domain; cd01212"
                     /db_xref="CDD:269923"
     Site            order(358,439,459)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269923"
     Site            order(428..433,446,468,472)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269923"
     CDS             1..495
                     /gene="Aplip1"
                     /coded_by="XM_034329079.2:53..1540"
                     /db_xref="GeneID:117606522"
ORIGIN      
        1 madtefeefr qyferlpqhl kaplsnytlv hdvmiddspt ssqgsdaevg rscdgvagds
       61 edeevvvihr hdnqsghtsg ddsedldhnc sivadsdfrl vtsrgrsagv ggpgpgggst
      121 tvsgnggrds vfsdvgdscs slsswptpeh vpsnrpgsgg terrrrrlpe ipktkksvgq
      181 mfmqssssla delnaaagst gstrphlslr kchsrlrhed sspdsermat dsghstahsp
      241 engpksvspi pcntlqntds aspsstpgsg gvpftqlell eathrglhkf vprhhdeidl
      301 eigdpiyvqk eaddlwcegv nlrtgrqgif psayavdmdy sdfdpsapkv kreryllgyl
      361 gsvetlahkg tgvvcqavrr ivgnsqespv sqscilevsd qglrmvdrsk prknqgpchd
      421 yfyslknvsf cafhprdhry lgfitkhptl qrfachvfig qestrpvaea vgrafhrfyt
      481 kfietafpve diyie
//
LOCUS       XP_034184976             491 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein Aplip1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184976
VERSION     XP_034184976.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329085.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184976.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..491
                     /product="JNK-interacting protein Aplip1 isoform X4"
                     /calculated_mol_wt=53590
     Region          279..333
                     /region_name="SH3_JIP1_like"
                     /note="Src homology 3 domain of JNK-interacting proteins 1
                     and 2, and similar domains; cd11801"
                     /db_xref="CDD:212735"
     Site            order(284..287,289..290,292..293,308,310..312,314,325,
                     327..330)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:212735"
     Site            order(284,286,289,293,311..312,327,329..330)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212735"
     Region          347..491
                     /region_name="PTB_JIP"
                     /note="JNK-interacting protein-like (JIP)
                     Phosphotyrosine-binding (PTB) domain; cd01212"
                     /db_xref="CDD:269923"
     Site            order(354,435,455)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269923"
     Site            order(424..429,442,464,468)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269923"
     CDS             1..491
                     /gene="Aplip1"
                     /coded_by="XM_034329085.2:53..1528"
                     /db_xref="GeneID:117606522"
ORIGIN      
        1 madtefeefr qyferlpqhl kaplsnytlv hdvmiddspt ssqgsdaevg rscdgvagds
       61 edeevvvihr hdnqsghtsg ddsedldhnc sivadsdfsr grsagvggpg pgggsttvsg
      121 nggrdsvfsd vgdscsslss wptpehvpsn rpgsggterr rrrlpeipkt kksvgqmfmq
      181 ssssladeln aaagstgstr phlslrkchs rlrhedsspd sermatdsgh stahspengp
      241 ksvspipcnt lqntdsasps stpgsggvpf tqlelleath rglhkfvprh hdeidleigd
      301 piyvqkeadd lwcegvnlrt grqgifpsay avdmdysdfd psapkvkrer yllgylgsve
      361 tlahkgtgvv cqavrrivgn sqespvsqsc ilevsdqglr mvdrskprkn qgpchdyfys
      421 lknvsfcafh prdhrylgfi tkhptlqrfa chvfigqest rpvaeavgra fhrfytkfie
      481 tafpvediyi e
//
LOCUS       XP_034180240             644 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604378 [Osmia lignaria lignaria].
ACCESSION   XP_034180240
VERSION     XP_034180240.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324349.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180240.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..644
                     /product="uncharacterized protein LOC117604378"
                     /calculated_mol_wt=70752
     Region          189..>325
                     /region_name="PHA03255"
                     /note="BDLF3; Provisional"
                     /db_xref="CDD:165513"
     CDS             1..644
                     /gene="LOC117604378"
                     /coded_by="XM_034324349.2:1..1935"
                     /db_xref="GeneID:117604378"
ORIGIN      
        1 mkklvimvai avsvtvghke rrnsalenfp pypdarqgls fheqhqpfre asfhpqqyqs
       61 yrhrpyrqdl ssfaqqlsyr dqslprdyss pknrpfytdg sffrdtsnyk gqssffsdga
      121 sfkdqtrlkn eilkdqtlnd qysyqqpsfr ddfrfadlyd kpissyreqq peefskesdy
      181 lkhgnhglgy ipmppvspyq nasplnegts ttttpsmvpt tthvpllqsn vssvapaisq
      241 vhtsatsepk maslkgshtv tmptvksdtl aevsttsspm ssdvtvmsvs teqssnlahp
      301 qmpaltteas lppvmatesp vlsfgrqsll piefqsskft tpssyyfspa vknklqqsll
      361 syllsqqekn plkineqstl lsnyvspdvl snsldnkipg pvlnyvlpae sknslknnwq
      421 tsllnyllqq esnrglsfqp stpstetvny lslatlaekp kltlptvpia tlsavsrplq
      481 tlnyvpvtmp rtpsfvtqds svsslpilpl gsnslstfss nladsqatgv fnslsgglsg
      541 giasgipgni attvpasipt lnfaqnfqhv dqtrqfepar nnpfstglql qlggfggidy
      601 tlrspnpvpr plelgiakvg lslpelprhq lstlskvpfg hqfw
//
LOCUS       XP_076546339             126 aa            linear   INV 03-SEP-2025
DEFINITION  longitudinals lacking protein, isoforms N/O/W/X/Y-like [Osmia
            lignaria lignaria].
ACCESSION   XP_076546339
VERSION     XP_076546339.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690224.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..126
                     /product="longitudinals lacking protein, isoforms
                     N/O/W/X/Y-like"
                     /calculated_mol_wt=15184
     Region          4..24
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(9,11,13,15..16,19..20,23,38,40,54..55,58..59,64,72,
                     74,76,78..79,82..83,86)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          33..65
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          67..86
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          96..113
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..126
                     /gene="LOC143305690"
                     /coded_by="XM_076690224.1:1..381"
                     /db_xref="GeneID:143305690"
ORIGIN      
        1 mwwcyqcgkq ymwrdslkkh lrvecgkdpt fecpicgrkf khkhlprltv knienlqykr
       61 rpvihkctrc gkgyqletsl rrhqrlecgv eprqscpicg kkfmhrfkmt hhlascrrkr
      121 dyygsi
//
LOCUS       XP_034181942            1628 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181942
VERSION     XP_034181942.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326051.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181942.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1628
                     /product="uncharacterized protein LOC117605123 isoform X1"
                     /calculated_mol_wt=186265
     CDS             1..1628
                     /gene="LOC117605123"
                     /coded_by="XM_034326051.2:1493..6379"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mnitsfvrtg nnekkgedlq klpqdtcnwk msytivkshq envmlrnkys yllyirrskl
       61 qnpcpadikm lkyitrldqd drdskfkyee ldadfeeyed qsdeyineld qleppklstw
      121 eeykklvdla qlnpkifkrs plvhltslsk iyintehsks phwnarakrs iiklgmqkni
      181 iiadstyvqf rnfklnklyk ktitlqnvst spakfqiepr pcnsnfnvvi krlesnrniv
      241 ppgmhfqliv ffrctdvdep eevlvmkveh gksliirlha yreppillgt cspskksldi
      301 lefnakylvq awrpetssse dsvnssedsv sysvrswdti hnkfagmtfd ckigfvgetv
      361 hvpikfknvg gdgrffamse idwatmhiqd itdtnvlkls rfnvwpayfi lksqeemilh
      421 isflpdfygv hvdkvyilcn ngtilateii gdgviyepff iqiskklkkd kiakksvdkq
      481 anycvqinas apnmfgqcti yvtntseism yfkwekrdiq tdenkmnqhk hfplefllir
      541 pdhgifapss thhfniiaey snlqpalyla vlqlyvedip eaaicdktqs rvkqctnkqr
      601 dclnsvdvwv adlevwlqyt tddqiksdqa fdeivellag kisdyndfik dqqeeteged
      661 mrtegeeyks tnlllidelf shydimnlkq fkpllwerki tmgiirptrt lyigieetcv
      721 ltvknlsdhs lnyswgdvgg edaekmkici cpekgelsgr ttekmkitlm plkegivqsl
      781 hmlcfignsq kiimlgiecn ieplyvtfyf plsdkqpvel knnfvriewr vnslqlaldl
      841 agkskkgmkl ldkyrareeq elmnanldqg dvlkdasagp sisevaeesg eqstlstrts
      901 eiiqlsklat gngkysqeki vscedivpff eitlpftqqp tvmeflnlpl rtgtsklykt
      961 iviskwngiy ykfsvekktf iiknetsipt nfrlrlknfy pvmcscegis ledriksiyk
     1021 rvhcqekdli eeimyrvkqp nsgvviyvdp lssnigpfea vpvdiyafgd twgiyvdkle
     1081 initglplyt ldicvqivgs piwvsisdrn qskvpvlkyg tiikgirlqe rkivlrntsv
     1141 vpiaidwhsf litsvtesmp fnvtvnvctl ftdklasklr asksksaset ehvkfysste
     1201 nlnernstgs sissditaht hsgssymats wksdgeslqt teisveytse dtledkktad
     1261 sdisshntte dvedtefris ilpyygpvdt iactvaprem filpndtafl risiqpdkyn
     1321 nkvkkiyeek flskilgflr iapsdkyrdn cyfrqdgiyf ppveiditad iiqprvtfsi
     1381 pkanrifkcc ahdvmqsktk kleliqtffl hncapetiev mletsdpfhi ksvaiyaetd
     1441 pckftgtici sskgcaqvki mcivdaelie tiihthkcqd lydsvitltq plqiihtdkr
     1501 yqkielflqv wlpilklssy alnfglvyig dtkklmivik nlsvcsqyik vnqkfknndf
     1561 ildqkegils sylngknssl mltvsfqpkk pgqsaetlei itaipsntee ceiygegtld
     1621 ekyhapgv
//
LOCUS       XP_034181953            1626 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181953
VERSION     XP_034181953.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326062.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181953.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1626
                     /product="uncharacterized protein LOC117605123 isoform X2"
                     /calculated_mol_wt=186051
     CDS             1..1626
                     /gene="LOC117605123"
                     /coded_by="XM_034326062.2:1493..6373"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mnitsfvrtg nnekkgedlq klpqdtcnwk msytivkshq envmlrnkys yllyirrskl
       61 qnpcpadikm lkyitrldqd drdskfkyee ldadfeeyed qsdeyineld qleppklstw
      121 eeykklvdla qlnpkifkrs plvhltslsk iyintehsks phwnarakrs iiklgmqkni
      181 iiadstyvqf rnfklnklyk ktitlqnvst spakfqiepr pcnsnfnvvi krlesnrniv
      241 ppgmhfqliv ffrctdvdep eevlvmkveh gksliirlha yreppillgt cspskksldi
      301 lefnakylvq awrpetssse dsssedsvsy svrswdtihn kfagmtfdck igfvgetvhv
      361 pikfknvggd grffamseid watmhiqdit dtnvlklsrf nvwpayfilk sqeemilhis
      421 flpdfygvhv dkvyilcnng tilateiigd gviyepffiq iskklkkdki akksvdkqan
      481 ycvqinasap nmfgqctiyv tntseismyf kwekrdiqtd enkmnqhkhf plefllirpd
      541 hgifapssth hfniiaeysn lqpalylavl qlyvedipea aicdktqsrv kqctnkqrdc
      601 lnsvdvwvad levwlqyttd dqiksdqafd eivellagki sdyndfikdq qeetegedmr
      661 tegeeykstn lllidelfsh ydimnlkqfk pllwerkitm giirptrtly igieetcvlt
      721 vknlsdhsln yswgdvgged aekmkicicp ekgelsgrtt ekmkitlmpl kegivqslhm
      781 lcfignsqki imlgiecnie plyvtfyfpl sdkqpvelkn nfvriewrvn slqlaldlag
      841 kskkgmklld kyrareeqel mnanldqgdv lkdasagpsi sevaeesgeq stlstrtsei
      901 iqlsklatgn gkysqekivs cedivpffei tlpftqqptv meflnlplrt gtsklyktiv
      961 iskwngiyyk fsvekktfii knetsiptnf rlrlknfypv mcscegisle driksiykrv
     1021 hcqekdliee imyrvkqpns gvviyvdpls snigpfeavp vdiyafgdtw giyvdklein
     1081 itglplytld icvqivgspi wvsisdrnqs kvpvlkygti ikgirlqerk ivlrntsvvp
     1141 iaidwhsfli tsvtesmpfn vtvnvctlft dklasklras ksksaseteh vkfysstenl
     1201 nernstgssi ssditahths gssymatswk sdgeslqtte isveytsedt ledkktadsd
     1261 isshnttedv edtefrisil pyygpvdtia ctvapremfi lpndtaflri siqpdkynnk
     1321 vkkiyeekfl skilgflria psdkyrdncy frqdgiyfpp veiditadii qprvtfsipk
     1381 anrifkccah dvmqsktkkl eliqtfflhn capetievml etsdpfhiks vaiyaetdpc
     1441 kftgticiss kgcaqvkimc ivdaelieti ihthkcqdly dsvitltqpl qiihtdkryq
     1501 kielflqvwl pilklssyal nfglvyigdt kklmiviknl svcsqyikvn qkfknndfil
     1561 dqkegilssy lngknsslml tvsfqpkkpg qsaetleiit aipsnteece iygegtldek
     1621 yhapgv
//
LOCUS       XP_034181963            1606 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181963
VERSION     XP_034181963.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326072.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181963.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1606
                     /product="uncharacterized protein LOC117605123 isoform X3"
                     /calculated_mol_wt=183686
     CDS             1..1606
                     /gene="LOC117605123"
                     /coded_by="XM_034326072.2:1493..6313"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mnitsfvrtg nnekkgedlq klpqdtcnwk msytivkshq envmlrnkys yllyirrskl
       61 qnpcpadikm lkyitrldqd drdskfkyee ldadfeeyed qsdeyineld qleppklstw
      121 eeykklvdla qlnpkifkrs plvhltslsk iyintehsks phwnarakrs iiklgmqkni
      181 iiadstyvqf rnfklnklyk ktitlqnvst spakfqiepr pcnsnfnvvi krlesnrniv
      241 ppgmhfqliv ffrctdvdep eevlvmkveh gksliirlha yreppillgt cspskksldi
      301 lefnakylvq awrpetssse dsvnssedsv sysvrswdti hnkfagmtfd ckigfvgetv
      361 hvpikfknvg gdgrffamse idwatmhiqd itdtnvlkls rfnvwpayfi lksqeemilh
      421 isflpdfygv hvdkvyilcn ngtilateii gdgviyepff iqiskklkkd kiakksvdkq
      481 anycvqinas apnmfgqcti yvtntseism yfkwekrdiq tdenkmnqhk hfplefllir
      541 pdhgifapss thhfniiaey snlqpalyla vlqlyvedip eaaicdktqs rvkqctnkqr
      601 dclnsvdvwv adlevwlqyt tddqiksdqa fdeivellag kisdyndfik dqqeeteged
      661 mrtegeeyks tnlllidelf shydimnlkq fkpllwerki tmgiirptrt lyigieetcv
      721 ltvknlsdhs lnyswgdvgg edaekmkici cpekgelsgr ttekmkitlm plkegivqsl
      781 hmlcfignsq kiimlgiecn ieplyvtfyf plsdkqpvel knnfvriewr vnslqlaldl
      841 agkskkgmkl ldkyrareeq elmnanldqg dvlkdasagp sisevaeesg eqstlstrts
      901 eiiqlsklat gngkysqeki vscedivpff eitlpftqqp tvmeflnlpl rtvekktfii
      961 knetsiptnf rlrlknfypv mcscegisle driksiykrv hcqekdliee imyrvkqpns
     1021 gvviyvdpls snigpfeavp vdiyafgdtw giyvdklein itglplytld icvqivgspi
     1081 wvsisdrnqs kvpvlkygti ikgirlqerk ivlrntsvvp iaidwhsfli tsvtesmpfn
     1141 vtvnvctlft dklasklras ksksaseteh vkfysstenl nernstgssi ssditahths
     1201 gssymatswk sdgeslqtte isveytsedt ledkktadsd isshnttedv edtefrisil
     1261 pyygpvdtia ctvapremfi lpndtaflri siqpdkynnk vkkiyeekfl skilgflria
     1321 psdkyrdncy frqdgiyfpp veiditadii qprvtfsipk anrifkccah dvmqsktkkl
     1381 eliqtfflhn capetievml etsdpfhiks vaiyaetdpc kftgticiss kgcaqvkimc
     1441 ivdaelieti ihthkcqdly dsvitltqpl qiihtdkryq kielflqvwl pilklssyal
     1501 nfglvyigdt kklmiviknl svcsqyikvn qkfknndfil dqkegilssy lngknsslml
     1561 tvsfqpkkpg qsaetleiit aipsnteece iygegtldek yhapgv
//
LOCUS       XP_034181972            1583 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181972
VERSION     XP_034181972.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326081.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181972.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1583
                     /product="uncharacterized protein LOC117605123 isoform X4"
                     /calculated_mol_wt=181506
     CDS             1..1583
                     /gene="LOC117605123"
                     /coded_by="XM_034326081.2:1493..6244"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mnitsfvrtg nnekkgedlq klpqdtcnwk msytivkshq envmlrnkys yllyirrskl
       61 qnpcpadikm lkyitrldqd drdskfkyee ldadfeeyed qsdeyineld qleppklstw
      121 eeykklvdla qlnpkifkrs plvhltslsk iyintehsks phwnarakrs iiklgmqkni
      181 iiadstyvqf rnfklnklyk ktitlqnvst spakfqiepr pcnsnfnvvi krlesnrniv
      241 ppgmhfqliv ffrctdvdep eevlvmkveh gksliirlha yreppillgt cspskksldi
      301 lefnakylvq awrpetssse dsvnssedsv sysvrswdti hnkfagmtfd ckigfvgetv
      361 hvpikfknvg gdgrffamse idwatmhiqd itdtnvlkls rfnvwpayfi lksqeemilh
      421 isflpdfygv hvdkvyilcn ngtilateii gdgviyepff iqiskklkkd kiakksvdkq
      481 anycvqinas apnmfgqcti yvtntseism yfkwekrdiq tdenkmnqhk hfplefllir
      541 pdhgifapss thhfniiaey snlqpalyla vlqlyvedip eaaicdktqs rvkqctnkqr
      601 dclnsvdvwv adlevwlqyt tddqiksdqa fdeivellag kisdyndfik dqqeeteged
      661 mrtegeeyks tnlllidelf shydimnlkq fkpllwerki tmgiirptrt lyigieetcv
      721 ltvknlsdhs lnyswgdvgg edaekmkici cpekgelsgr ttekmkitlm plkegivqsl
      781 hmlcfignsq kiimlgiecn ieplyvtfyf plsdkqpvel knnfvriewr vnslqlaldl
      841 agkskkgmkl ldkyrareeq elmnanldqg dvlkdasagp sisevaeesg eqstlstrts
      901 eiiqlsklat gngkysqeki vscedivpff eitlpftqqp tvmeflnlpl rtgtsklykt
      961 iviskwngiy ykfsvekktf iiknetsipt nfrlrlknfy pvmcscegis ledriksiyk
     1021 rvhcqekdli eeimyrvkqp nsgvviyvdp lssnigpfea vpvdiyafgd twgiyvdkle
     1081 initglplyt ldicvqivgs piwvsisdrn qskvpvlkyg tiikgirlqe rkivlrntsv
     1141 vpiaidwhsf litsvtesmp fnvtvnvctl ftdklasklr asksksaset ehvkfysste
     1201 nlnernstgs sissditaht hsgssymats wksdgeslqt teisveytse dtledkktad
     1261 sdisshntte dvedtefris ilpyygpvdt iactvaprem filpndtafl risiqpdkyn
     1321 nkvkkiyeek flskilgflr iapsdkyrdn cyfrqdgiyf ppveiditad iiqprvtfsi
     1381 pkanrifkcc ahdvmqsktk kleliqtffl hncapetiev mletsdpfhi ksvaiyaetd
     1441 pckftgtici sskgcaqvki mcivdaelie tiihthkcqd lydsvitltq plqiihtdkr
     1501 yqkielflqv wlpilklssy alnfglvyig dtkklmivik nlsvcsqyik vnqkfknndf
     1561 ildqkegils sylnenlgnq lkh
//
LOCUS       XP_034181980            1559 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181980
VERSION     XP_034181980.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326089.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181980.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1559
                     /product="uncharacterized protein LOC117605123 isoform X5"
                     /calculated_mol_wt=178102
     CDS             1..1559
                     /gene="LOC117605123"
                     /coded_by="XM_034326089.2:194..4873"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mlkyitrldq ddrdskfkye eldadfeeye dqsdeyinel dqleppklst weeykklvdl
       61 aqlnpkifkr splvhltsls kiyintehsk sphwnarakr siiklgmqkn iiiadstyvq
      121 frnfklnkly kktitlqnvs tspakfqiep rpcnsnfnvv ikrlesnrni vppgmhfqli
      181 vffrctdvde peevlvmkve hgksliirlh ayreppillg tcspskksld ilefnakylv
      241 qawrpetsss edsvnsseds vsysvrswdt ihnkfagmtf dckigfvget vhvpikfknv
      301 ggdgrffams eidwatmhiq ditdtnvlkl srfnvwpayf ilksqeemil hisflpdfyg
      361 vhvdkvyilc nngtilatei igdgviyepf fiqiskklkk dkiakksvdk qanycvqina
      421 sapnmfgqct iyvtntseis myfkwekrdi qtdenkmnqh khfpleflli rpdhgifaps
      481 sthhfniiae ysnlqpalyl avlqlyvedi peaaicdktq srvkqctnkq rdclnsvdvw
      541 vadlevwlqy ttddqiksdq afdeivella gkisdyndfi kdqqeetege dmrtegeeyk
      601 stnlllidel fshydimnlk qfkpllwerk itmgiirptr tlyigieetc vltvknlsdh
      661 slnyswgdvg gedaekmkic icpekgelsg rttekmkitl mplkegivqs lhmlcfigns
      721 qkiimlgiec nieplyvtfy fplsdkqpve lknnfvriew rvnslqlald lagkskkgmk
      781 lldkyraree qelmnanldq gdvlkdasag psisevaees geqstlstrt seiiqlskla
      841 tgngkysqek ivscedivpf feitlpftqq ptvmeflnlp lrtgtsklyk tiviskwngi
      901 yykfsvekkt fiiknetsip tnfrlrlknf ypvmcscegi sledriksiy krvhcqekdl
      961 ieeimyrvkq pnsgvviyvd plssnigpfe avpvdiyafg dtwgiyvdkl einitglply
     1021 tldicvqivg spiwvsisdr nqskvpvlky gtiikgirlq erkivlrnts vvpiaidwhs
     1081 flitsvtesm pfnvtvnvct lftdklaskl rasksksase tehvkfysst enlnernstg
     1141 ssissditah thsgssymat swksdgeslq tteisveyts edtledkkta dsdisshntt
     1201 edvedtefri silpyygpvd tiactvapre mfilpndtaf lrisiqpdky nnkvkkiyee
     1261 kflskilgfl riapsdkyrd ncyfrqdgiy fppveidita diiqprvtfs ipkanrifkc
     1321 cahdvmqskt kkleliqtff lhncapetie vmletsdpfh iksvaiyaet dpckftgtic
     1381 isskgcaqvk imcivdaeli etiihthkcq dlydsvitlt qplqiihtdk ryqkielflq
     1441 vwlpilklss yalnfglvyi gdtkklmivi knlsvcsqyi kvnqkfknnd fildqkegil
     1501 ssylngknss lmltvsfqpk kpgqsaetle iitaipsnte eceiygegtl dekyhapgv
//
LOCUS       XP_034181989            1528 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181989
VERSION     XP_034181989.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181989.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1528
                     /product="uncharacterized protein LOC117605123 isoform X6"
                     /calculated_mol_wt=174735
     CDS             1..1528
                     /gene="LOC117605123"
                     /coded_by="XM_034326098.2:1493..6079"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mnitsfvrtg nnekkgedlq klpqdtcnwk msytivkshq envmlrnkys yllyirrskl
       61 qnpcpadikm lkyitrldqd drdskfkyee ldadfeeyed qsdeyineld qleppklstw
      121 eeykklvdla qlnpkifkrs plvhltslsk iyintehsks phwnarakrs iiklgmqkni
      181 iiadstyvqf rnfklnklyk ktitlqnvst spakfqiepr pcnsnfnvvi krlesnrniv
      241 ppgmhfqliv ffrctdvdep eevlvmkveh gksliirlha yreppillgt cspskksldi
      301 lefnakylvq awrpetssse dsvnssedsv sysvrswdti hnkfagmtfd ckigfvgetv
      361 hvpikfknvg gdgrffamse idwatmhiqd itdtnvlkls rfnvwpayfi lksqeemilh
      421 isflpdfygv hvdkvyilcn ngtilateii gdgviyepff iqiskklkkd kiakksvdkq
      481 anycvqinas apnmfgqcti yvtntseism yfkwekrdiq tdenkmnqhk hfplefllir
      541 pdhgifapss thhfniiaey snlqpalyla vlqlyvedip eaaicdktqs rvkqctnkqr
      601 dclnsvdvwv adlevwlqyt tddqiksdqa fdeivellag kisdyndfik dqqeeteged
      661 mrtegeeyks tnllgivqsl hmlcfignsq kiimlgiecn ieplyvtfyf plsdkqpvel
      721 knnfvriewr vnslqlaldl agkskkgmkl ldkyrareeq elmnanldqg dvlkdasagp
      781 sisevaeesg eqstlstrts eiiqlsklat gngkysqeki vscedivpff eitlpftqqp
      841 tvmeflnlpl rtgtsklykt iviskwngiy ykfsvekktf iiknetsipt nfrlrlknfy
      901 pvmcscegis ledriksiyk rvhcqekdli eeimyrvkqp nsgvviyvdp lssnigpfea
      961 vpvdiyafgd twgiyvdkle initglplyt ldicvqivgs piwvsisdrn qskvpvlkyg
     1021 tiikgirlqe rkivlrntsv vpiaidwhsf litsvtesmp fnvtvnvctl ftdklasklr
     1081 asksksaset ehvkfysste nlnernstgs sissditaht hsgssymats wksdgeslqt
     1141 teisveytse dtledkktad sdisshntte dvedtefris ilpyygpvdt iactvaprem
     1201 filpndtafl risiqpdkyn nkvkkiyeek flskilgflr iapsdkyrdn cyfrqdgiyf
     1261 ppveiditad iiqprvtfsi pkanrifkcc ahdvmqsktk kleliqtffl hncapetiev
     1321 mletsdpfhi ksvaiyaetd pckftgtici sskgcaqvki mcivdaelie tiihthkcqd
     1381 lydsvitltq plqiihtdkr yqkielflqv wlpilklssy alnfglvyig dtkklmivik
     1441 nlsvcsqyik vnqkfknndf ildqkegils sylngknssl mltvsfqpkk pgqsaetlei
     1501 itaipsntee ceiygegtld ekyhapgv
//
LOCUS       XP_034181998            1401 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181998
VERSION     XP_034181998.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326107.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181998.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1401
                     /product="uncharacterized protein LOC117605123 isoform X7"
                     /calculated_mol_wt=160635
     CDS             1..1401
                     /gene="LOC117605123"
                     /coded_by="XM_034326107.2:1493..5698"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mnitsfvrtg nnekkgedlq klpqdtcnwk msytivkshq envmlrnkys yllyirrskl
       61 qnpcpadikm lkyitrldqd drdskfkyee ldadfeeyed qsdeyineld qleppklstw
      121 eeykklvdla qlnpkifkrs plvhltslsk iyintehsks phwnarakrs iiklgmqkni
      181 iiadstyvqf rnfklnklyk ktitlqnvst spakfqiepr pcnsnfnvvi krlesnrniv
      241 ppgmhfqliv ffrctdvdep eevlvmkveh gksliirlha yreppillgt cspskksldi
      301 lefnakylvq awrpetssse dsvnssedsv sysvrswdti hnkfagmtfd ckigfvgetv
      361 hvpikfknvg gdgrffamse idwatmhiqd itdtnvlkls rfnvwpayfi lksqeemilh
      421 isflpdfygv hvdkvyilcn ngtilateii gdgviyepff iqiskklkkd kiakksvdkq
      481 anycvqinas apnmfgqcti yvtntseism yfkwekrdiq tdenkmnqhk hfplefllir
      541 pdhgifapss thhfniiaey snlqpalyla vlqlyvedip eaaicdktqs rvkqctnkqr
      601 dclnsvdvwv adlevwlqyt tddqiksdqa fdeivellag kisdyndfik dqqeeteged
      661 mrtegeeyks tnlllidelf shydimnlkq fkpllwerki tmgiirptrt lyigieetcv
      721 ltvknlsdhs lnyswgdvgg edaekmkici cpekgelsgr ttekmkitlm plkegivqsl
      781 hmlcfignsq kiimlgiecn ieplyvtfyf plsdkqpvel knnfvriewr vnslqlaldl
      841 agkskkgmkl ldkyrareeq elmnanldqg dvlkdasagp sisevaeesg eqstlstrts
      901 eiiqlsklat gngkysqeki vscedivpff eitlpftqqp tvmeflnlpl rtgtsklykt
      961 iviskwngiy ykfsvekktf iiknetsipt nfrlrlknfy pvmcscegis ledriksiyk
     1021 rvhcqekdli eeimyrvkqp nsgvviyvdp lssnigpfea vpvdiyafgd twgiyvdkle
     1081 initglplyt ldicvqivgs piwvsisdrn qskvpvlkyg tiikgirlqe rkivlrntsv
     1141 vpiaidwhsf litsvtesmp fnvtvnvctl ftdklasklr asksksaset ehvkfysste
     1201 nlnernstgs sissditaht hsgssymats wksdgeslqt teisveytse dtledkktad
     1261 sdisshntte dvedtefris ilpyygpvdt iactvaprem filpndtafl risiqpdkyn
     1321 nkvkkiyeek flskilgflr iapsdkyrdn cyfrqdgiyf ppveiditad iiqprvtfsi
     1381 pkanrifkcc ahdidsnill t
//
LOCUS       XP_034182007            1385 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182007
VERSION     XP_034182007.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326116.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182007.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1385
                     /product="uncharacterized protein LOC117605123 isoform X8"
                     /calculated_mol_wt=157582
     CDS             1..1385
                     /gene="LOC117605123"
                     /coded_by="XM_034326116.2:258..4415"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mhfqlivffr ctdvdepeev lvmkvehgks liirlhayre ppillgtcsp skksldilef
       61 nakylvqawr petsssedsv nssedsvsys vrswdtihnk fagmtfdcki gfvgetvhvp
      121 ikfknvggdg rffamseidw atmhiqditd tnvlklsrfn vwpayfilks qeemilhisf
      181 lpdfygvhvd kvyilcnngt ilateiigdg viyepffiqi skklkkdkia kksvdkqany
      241 cvqinasapn mfgqctiyvt ntseismyfk wekrdiqtde nkmnqhkhfp lefllirpdh
      301 gifapssthh fniiaeysnl qpalylavlq lyvedipeaa icdktqsrvk qctnkqrdcl
      361 nsvdvwvadl evwlqyttdd qiksdqafde ivellagkis dyndfikdqq eetegedmrt
      421 egeeykstnl llidelfshy dimnlkqfkp llwerkitmg iirptrtlyi gieetcvltv
      481 knlsdhslny swgdvggeda ekmkicicpe kgelsgrtte kmkitlmplk egivqslhml
      541 cfignsqkii mlgiecniep lyvtfyfpls dkqpvelknn fvriewrvns lqlaldlagk
      601 skkgmklldk yrareeqelm nanldqgdvl kdasagpsis evaeesgeqs tlstrtseii
      661 qlsklatgng kysqekivsc edivpffeit lpftqqptvm eflnlplrtg tsklyktivi
      721 skwngiyykf svekktfiik netsiptnfr lrlknfypvm cscegisled riksiykrvh
      781 cqekdlieei myrvkqpnsg vviyvdplss nigpfeavpv diyafgdtwg iyvdkleini
      841 tglplytldi cvqivgspiw vsisdrnqsk vpvlkygtii kgirlqerki vlrntsvvpi
      901 aidwhsflit svtesmpfnv tvnvctlftd klasklrask sksasetehv kfysstenln
      961 ernstgssis sditahthsg ssymatswks dgeslqttei sveytsedtl edkktadsdi
     1021 sshnttedve dtefrisilp yygpvdtiac tvapremfil pndtaflris iqpdkynnkv
     1081 kkiyeekfls kilgflriap sdkyrdncyf rqdgiyfppv eiditadiiq prvtfsipka
     1141 nrifkccahd vmqsktkkle liqtfflhnc apetievmle tsdpfhiksv aiyaetdpck
     1201 ftgticissk gcaqvkimci vdaelietii hthkcqdlyd svitltqplq iihtdkryqk
     1261 ielflqvwlp ilklssyaln fglvyigdtk klmiviknls vcsqyikvnq kfknndfild
     1321 qkegilssyl ngknsslmlt vsfqpkkpgq saetleiita ipsnteecei ygegtldeky
     1381 hapgv
//
LOCUS       XP_034182018            1348 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605123 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182018
VERSION     XP_034182018.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326127.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182018.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1348
                     /product="uncharacterized protein LOC117605123 isoform X9"
                     /calculated_mol_wt=154478
     CDS             1..1348
                     /gene="LOC117605123"
                     /coded_by="XM_034326127.2:1493..5539"
                     /db_xref="GeneID:117605123"
ORIGIN      
        1 mnitsfvrtg nnekkgedlq klpqdtcnwk msytivkshq envmlrnkys yllyirrskl
       61 qnpcpadikm lkyitrldqd drdskfkyee ldadfeeyed qsdeyineld qleppklstw
      121 eeykklvdla qlnpkifkrs plvhltslsk iyintehsks phwnarakrs iiklgmqkni
      181 iiadstyvqf rnfklnklyk ktitlqnvst spakfqiepr pcnsnfnvvi krlesnrniv
      241 ppgmhfqliv ffrctdvdep eevlvmkveh gksliirlha yreppillgt cspskksldi
      301 lefnakylvq awrpetssse dsvnssedsv sysvrswdti hnkfagmtfd ckigfvgetv
      361 hvpikfknvg gdgrffamse idwatmhiqd itdtnvlkls rfnvwpayfi lksqeemilh
      421 isflpdfygv hvdkvyilcn ngtilateii gdgviyepff iqiskklkkd kiakksvdkq
      481 anycvqinas apnmfgqcti yvtntseism yfkwekrdiq tdenkmnqhk hfplefllir
      541 pdhgifapss thhfniiaey snlqpalyla vlqlyvedip eaaicdktqs rvkqctnkqr
      601 dclnsvdvwv adlevwlqyt tddqiksdqa fdeivellag kisdyndfik dqqeeteged
      661 mrtegeeyks tnlllidelf shydimnlkq fkpllwerki tmgiirptrt lyigieetcv
      721 ltvknlsdhs lnyswgdvgg edaekmkici cpekgelsgr ttekmkitlm plkegivqsl
      781 hmlcfignsq kiimlgiecn ieplyvtfyf plsdkqpvel knnfvriewr vnslqlaldl
      841 agkskkgmkl ldkyrareeq elmnanldqg dvlkdasagp sisevaeesg eqstlstrts
      901 eiiqlsklat gngkysqeki vscedivpff eitlpftqqp tvmeflnlpl rtgtsklykt
      961 iviskwngiy ykfsvekktf iiknetsipt nfrlrlknfy pvmcscegis ledriksiyk
     1021 rvhcqekdli eeimyrvkqp nsgvviyvdp lssnigpfea vpvdiyafgd twgiyvdkle
     1081 initglplyt ldicvqivgs piwvsisdrn qskvpvlkyg tiikgirlqe rkivlrntsv
     1141 vpiaidwhsf litsvtesmp fnvtvnvctl ftdklasklr asksksaset ehvkfysste
     1201 nlnernstgs sissditaht hsgssymats wksdgeslqt teisveytse dtledkktad
     1261 sdisshntte dvedtefris ilpyygpvdt iactvaprem filpndtafl risiqpdkyn
     1321 nkvkkiyeek flskilgflr iapsdkfv
//
LOCUS       XP_034182029            1545 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 10-like protein isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034182029
VERSION     XP_034182029.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182029.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1545
                     /product="rho guanine nucleotide exchange factor 10-like
                     protein isoform X1"
                     /calculated_mol_wt=172337
     Region          467..648
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          671..815
                     /region_name="PH_19"
                     /note="PH domain; pfam19057"
                     /db_xref="CDD:465965"
     Region          <814..879
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          942..1303
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1545
                     /gene="LOC117605150"
                     /coded_by="XM_034326138.2:189..4826"
                     /db_xref="GeneID:117605150"
ORIGIN      
        1 massvggpgm asgvptprrv nlefpppppy ppphsqmacs glvdpaptla vpppspahqh
       61 plqdysyayy epgpsrlhgt ypaevgmnrv qqqqqqqqqq lrpetfkrht ymtrygteen
      121 iyeeiseinr qchralhgsr rslvaeevrr vqsrhrrvlg elnlsveaml mptvvdnnde
      181 depqdqrgas teellssvsp tddllspvgc dmdsgfsgss sasyrsglgs lrrgmgktst
      241 pelpacqrkt kaamiwkkgw kgwkklhsfg nnsnktdepr srcrswddvg ptkmetvgqt
      301 hrqhpsleat rsntstqsar sedswcsasd hdlssddese ksnisiksnc qlrntlhkar
      361 tlcdkwrsqn mrvnnadpld spgnhgrlsr wfsirrgstq qydvdssdtv sltspiktpq
      421 mpqlceveee nsaivqfqcm qqrrqtppal pptppnltpq qlkrrhivaa ivhsensyvs
      481 tlqrlvndyk kpleessppi lsqakiatlf hrlpeilqch tlfrialaec vrswdkdekl
      541 gdvfvasfsk aivldiysgf innfsvamdl akqeskrkta ladffkvkqi sahdrlsffg
      601 lmvkpvqrfp qfilflqdll khtpqghhdr mslqlaltql eslaemlner kreaeqfqaf
      661 kemlrhvsgk lshrplssss rylirednvt qlefnqngmi tkskrrrlll lndlvvcvsv
      721 tprstedfsg serltlkwty pvsdieiqdt stsptlsrll taglnkggsl ksdksgeggq
      781 agadnlcvem ndlmhdyevm srisdlvaql kgkyegmtle ktkqilqsiq lsiqqkdedm
      841 vwadscclql vtkqgqmytf qtenplvkkd witelrlaql aldpnnspsw evpeqeqrps
      901 tkmplfvssq qvyhsqhqte vrcgcyyttq nsrptrrrgr sqsylwictg dgisshitvf
      961 gqsttasttl lkqvtafdlv etrvaaiefi kgvssdplsl andlvwmgtd srkiiiyaas
     1021 epekqeqlgn ysvsgpviqi khhcdnvfva lgigllllfk rqadgtwnlr dpfvislgse
     1081 pvsclmpint svyaacgkkv wvlhgvngdi tksfsvqheh vgsvklmahs gvglwvalkn
     1141 sstvclyhte tfkhlqdini asnvlrvtrs nnatnscgdn lnnnqttvtv tallackgll
     1201 wvgtnvgisl tiplprlegv piisgrvnis yhahfgpitf llaiqnpknt incptateei
     1261 ndeetvqlrs ketensrstr drasldssms nsiiklkqql agspvmlrrk rskeyeykgs
     1321 ktlprglgsg ggflsssmsg sqssgencdv yglygelmyv kdyenenssg idpiyeslrr
     1381 sepelaaipn kvstldrrlk mkitrprsld lsnwsvdsha sslytssgse enislktgkl
     1441 srnsstasrn gpyetttpns sivqsvpeai pppnnlkpng kkinktlqqv eqpkrtvltl
     1501 mggrgyinwr qlnaqsvpdk asksgytfkd pnsndahivl wemkl
//
LOCUS       XP_034182033            1544 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 10-like protein isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034182033
VERSION     XP_034182033.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182033.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1544
                     /product="rho guanine nucleotide exchange factor 10-like
                     protein isoform X2"
                     /calculated_mol_wt=172195
     Region          466..647
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          670..814
                     /region_name="PH_19"
                     /note="PH domain; pfam19057"
                     /db_xref="CDD:465965"
     Region          <813..878
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          941..1302
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1544
                     /gene="LOC117605150"
                     /coded_by="XM_034326142.2:189..4823"
                     /db_xref="GeneID:117605150"
ORIGIN      
        1 massvggpgm asgvptprrv nlefpppppy ppphsqmacs glvdpaptla vpppspahqh
       61 plqdysyayy epgpsrlhgt ypaevgmnrv qqqqqqqqqq lrpetfkrht ymtrygteen
      121 iyeeiseikq chralhgsrr slvaeevrrv qsrhrrvlge lnlsveamlm ptvvdnnded
      181 epqdqrgast eellssvspt ddllspvgcd mdsgfsgsss asyrsglgsl rrgmgktstp
      241 elpacqrktk aamiwkkgwk gwkklhsfgn nsnktdeprs rcrswddvgp tkmetvgqth
      301 rqhpsleatr sntstqsars edswcsasdh dlssddesek snisiksncq lrntlhkart
      361 lcdkwrsqnm rvnnadplds pgnhgrlsrw fsirrgstqq ydvdssdtvs ltspiktpqm
      421 pqlceveeen saivqfqcmq qrrqtppalp ptppnltpqq lkrrhivaai vhsensyvst
      481 lqrlvndykk pleessppil sqakiatlfh rlpeilqcht lfrialaecv rswdkdeklg
      541 dvfvasfska ivldiysgfi nnfsvamdla kqeskrktal adffkvkqis ahdrlsffgl
      601 mvkpvqrfpq filflqdllk htpqghhdrm slqlaltqle slaemlnerk reaeqfqafk
      661 emlrhvsgkl shrplssssr ylirednvtq lefnqngmit kskrrrllll ndlvvcvsvt
      721 prstedfsgs erltlkwtyp vsdieiqdts tsptlsrllt aglnkggslk sdksgeggqa
      781 gadnlcvemn dlmhdyevms risdlvaqlk gkyegmtlek tkqilqsiql siqqkdedmv
      841 wadscclqlv tkqgqmytfq tenplvkkdw itelrlaqla ldpnnspswe vpeqeqrpst
      901 kmplfvssqq vyhsqhqtev rcgcyyttqn srptrrrgrs qsylwictgd gisshitvfg
      961 qsttasttll kqvtafdlve trvaaiefik gvssdplsla ndlvwmgtds rkiiiyaase
     1021 pekqeqlgny svsgpviqik hhcdnvfval gigllllfkr qadgtwnlrd pfvislgsep
     1081 vsclmpints vyaacgkkvw vlhgvngdit ksfsvqhehv gsvklmahsg vglwvalkns
     1141 stvclyhtet fkhlqdinia snvlrvtrsn natnscgdnl nnnqttvtvt allackgllw
     1201 vgtnvgislt iplprlegvp iisgrvnisy hahfgpitfl laiqnpknti ncptateein
     1261 deetvqlrsk etensrstrd rasldssmsn siiklkqqla gspvmlrrkr skeyeykgsk
     1321 tlprglgsgg gflsssmsgs qssgencdvy glygelmyvk dyenenssgi dpiyeslrrs
     1381 epelaaipnk vstldrrlkm kitrprsldl snwsvdshas slytssgsee nislktgkls
     1441 rnsstasrng pyetttpnss ivqsvpeaip ppnnlkpngk kinktlqqve qpkrtvltlm
     1501 ggrgyinwrq lnaqsvpdka sksgytfkdp nsndahivlw emkl
//
LOCUS       XP_034182035            1544 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 10-like protein isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034182035
VERSION     XP_034182035.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326144.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182035.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1544
                     /product="rho guanine nucleotide exchange factor 10-like
                     protein isoform X3"
                     /calculated_mol_wt=172235
     Region          466..647
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          670..814
                     /region_name="PH_19"
                     /note="PH domain; pfam19057"
                     /db_xref="CDD:465965"
     Region          <813..878
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          941..1302
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1544
                     /gene="LOC117605150"
                     /coded_by="XM_034326144.2:189..4823"
                     /db_xref="GeneID:117605150"
ORIGIN      
        1 massvggpgm asgvptprrv nlefpppppy ppphsqmacs glvdpaptla vpppspahqh
       61 plqdysyayy epgpsrlhgt ypaevgmnrv qqqqqqqqqq lrpetfkrht ymtrygteen
      121 iyeeiseinr qchralhgsr rslvaeevrr vqsrhrrvlg elnlsveaml mptvvdnnde
      181 depqdqrgas teellssvsp tddllspvgc dmdsgfsgss sasyrsglgs lrrgmgktst
      241 pelpacqrkt kaamiwkkgw kgwkklhsfg nnsnktdepr srcrswddvg ptkmetvgqt
      301 hrqhpsleat rsntstqsar sedswcsasd hdlssddese ksnisinncq lrntlhkart
      361 lcdkwrsqnm rvnnadplds pgnhgrlsrw fsirrgstqq ydvdssdtvs ltspiktpqm
      421 pqlceveeen saivqfqcmq qrrqtppalp ptppnltpqq lkrrhivaai vhsensyvst
      481 lqrlvndykk pleessppil sqakiatlfh rlpeilqcht lfrialaecv rswdkdeklg
      541 dvfvasfska ivldiysgfi nnfsvamdla kqeskrktal adffkvkqis ahdrlsffgl
      601 mvkpvqrfpq filflqdllk htpqghhdrm slqlaltqle slaemlnerk reaeqfqafk
      661 emlrhvsgkl shrplssssr ylirednvtq lefnqngmit kskrrrllll ndlvvcvsvt
      721 prstedfsgs erltlkwtyp vsdieiqdts tsptlsrllt aglnkggslk sdksgeggqa
      781 gadnlcvemn dlmhdyevms risdlvaqlk gkyegmtlek tkqilqsiql siqqkdedmv
      841 wadscclqlv tkqgqmytfq tenplvkkdw itelrlaqla ldpnnspswe vpeqeqrpst
      901 kmplfvssqq vyhsqhqtev rcgcyyttqn srptrrrgrs qsylwictgd gisshitvfg
      961 qsttasttll kqvtafdlve trvaaiefik gvssdplsla ndlvwmgtds rkiiiyaase
     1021 pekqeqlgny svsgpviqik hhcdnvfval gigllllfkr qadgtwnlrd pfvislgsep
     1081 vsclmpints vyaacgkkvw vlhgvngdit ksfsvqhehv gsvklmahsg vglwvalkns
     1141 stvclyhtet fkhlqdinia snvlrvtrsn natnscgdnl nnnqttvtvt allackgllw
     1201 vgtnvgislt iplprlegvp iisgrvnisy hahfgpitfl laiqnpknti ncptateein
     1261 deetvqlrsk etensrstrd rasldssmsn siiklkqqla gspvmlrrkr skeyeykgsk
     1321 tlprglgsgg gflsssmsgs qssgencdvy glygelmyvk dyenenssgi dpiyeslrrs
     1381 epelaaipnk vstldrrlkm kitrprsldl snwsvdshas slytssgsee nislktgkls
     1441 rnsstasrng pyetttpnss ivqsvpeaip ppnnlkpngk kinktlqqve qpkrtvltlm
     1501 ggrgyinwrq lnaqsvpdka sksgytfkdp nsndahivlw emkl
//
LOCUS       XP_034182044            1281 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 10 isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182044
VERSION     XP_034182044.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326153.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1281
                     /product="rho guanine nucleotide exchange factor 10
                     isoform X4"
                     /calculated_mol_wt=143186
     Region          203..384
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          407..551
                     /region_name="PH_19"
                     /note="PH domain; pfam19057"
                     /db_xref="CDD:465965"
     Region          <550..615
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          678..1039
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1281
                     /gene="LOC117605150"
                     /coded_by="XM_034326153.2:2149..5994"
                     /db_xref="GeneID:117605150"
ORIGIN      
        1 meelhngvil ikyeprsrcr swddvgptkm etvgqthrqh psleatrsnt stqsarseds
       61 wcsasdhdls sddeseksni siksncqlrn tlhkartlcd kwrsqnmrvn nadpldspgn
      121 hgrlsrwfsi rrgstqqydv dssdtvslts piktpqmpql ceveeensai vqfqcmqqrr
      181 qtppalpptp pnltpqqlkr rhivaaivhs ensyvstlqr lvndykkple essppilsqa
      241 kiatlfhrlp eilqchtlfr ialaecvrsw dkdeklgdvf vasfskaivl diysgfinnf
      301 svamdlakqe skrktaladf fkvkqisahd rlsffglmvk pvqrfpqfil flqdllkhtp
      361 qghhdrmslq laltqlesla emlnerkrea eqfqafkeml rhvsgklshr plssssryli
      421 rednvtqlef nqngmitksk rrrllllndl vvcvsvtprs tedfsgserl tlkwtypvsd
      481 ieiqdtstsp tlsrlltagl nkggslksdk sgeggqagad nlcvemndlm hdyevmsris
      541 dlvaqlkgky egmtlektkq ilqsiqlsiq qkdedmvwad scclqlvtkq gqmytfqten
      601 plvkkdwite lrlaqlaldp nnspswevpe qeqrpstkmp lfvssqqvyh sqhqtevrcg
      661 cyyttqnsrp trrrgrsqsy lwictgdgis shitvfgqst tasttllkqv tafdlvetrv
      721 aaiefikgvs sdplslandl vwmgtdsrki iiyaasepek qeqlgnysvs gpviqikhhc
      781 dnvfvalgig llllfkrqad gtwnlrdpfv islgsepvsc lmpintsvya acgkkvwvlh
      841 gvngditksf svqhehvgsv klmahsgvgl wvalknsstv clyhtetfkh lqdiniasnv
      901 lrvtrsnnat nscgdnlnnn qttvtvtall ackgllwvgt nvgisltipl prlegvpiis
      961 grvnisyhah fgpitfllai qnpkntincp tateeindee tvqlrskete nsrstrdras
     1021 ldssmsnsii klkqqlagsp vmlrrkrske yeykgsktlp rglgsgggfl sssmsgsqss
     1081 gencdvygly gelmyvkdye nenssgidpi yeslrrsepe laaipnkvst ldrrlkmkit
     1141 rprsldlsnw svdshassly tssgseenis lktgklsrns stasrngpye tttpnssivq
     1201 svpeaipppn nlkpngkkin ktlqqveqpk rtvltlmggr gyinwrqlna qsvpdkasks
     1261 gytfkdpnsn dahivlwemk l
//
LOCUS       XP_034182052            1276 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 10 isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182052
VERSION     XP_034182052.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326161.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1276
                     /product="rho guanine nucleotide exchange factor 10
                     isoform X5"
                     /calculated_mol_wt=142524
     Region          198..379
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          402..546
                     /region_name="PH_19"
                     /note="PH domain; pfam19057"
                     /db_xref="CDD:465965"
     Region          <545..610
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          673..1034
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1276
                     /gene="LOC117605150"
                     /coded_by="XM_034326161.2:2301..6131"
                     /db_xref="GeneID:117605150"
ORIGIN      
        1 msftliadep rsrcrswddv gptkmetvgq thrqhpslea trsntstqsa rsedswcsas
       61 dhdlssddes eksnisiksn cqlrntlhka rtlcdkwrsq nmrvnnadpl dspgnhgrls
      121 rwfsirrgst qqydvdssdt vsltspiktp qmpqlcevee ensaivqfqc mqqrrqtppa
      181 lpptppnltp qqlkrrhiva aivhsensyv stlqrlvndy kkpleesspp ilsqakiatl
      241 fhrlpeilqc htlfrialae cvrswdkdek lgdvfvasfs kaivldiysg finnfsvamd
      301 lakqeskrkt aladffkvkq isahdrlsff glmvkpvqrf pqfilflqdl lkhtpqghhd
      361 rmslqlaltq leslaemlne rkreaeqfqa fkemlrhvsg klshrplsss srylirednv
      421 tqlefnqngm itkskrrrll llndlvvcvs vtprstedfs gserltlkwt ypvsdieiqd
      481 tstsptlsrl ltaglnkggs lksdksgegg qagadnlcve mndlmhdyev msrisdlvaq
      541 lkgkyegmtl ektkqilqsi qlsiqqkded mvwadscclq lvtkqgqmyt fqtenplvkk
      601 dwitelrlaq laldpnnsps wevpeqeqrp stkmplfvss qqvyhsqhqt evrcgcyytt
      661 qnsrptrrrg rsqsylwict gdgisshitv fgqsttastt llkqvtafdl vetrvaaief
      721 ikgvssdpls landlvwmgt dsrkiiiyaa sepekqeqlg nysvsgpviq ikhhcdnvfv
      781 algigllllf krqadgtwnl rdpfvislgs epvsclmpin tsvyaacgkk vwvlhgvngd
      841 itksfsvqhe hvgsvklmah sgvglwvalk nsstvclyht etfkhlqdin iasnvlrvtr
      901 snnatnscgd nlnnnqttvt vtallackgl lwvgtnvgis ltiplprleg vpiisgrvni
      961 syhahfgpit fllaiqnpkn tincptatee indeetvqlr sketensrst rdrasldssm
     1021 snsiiklkqq lagspvmlrr krskeyeykg sktlprglgs gggflsssms gsqssgencd
     1081 vyglygelmy vkdyenenss gidpiyeslr rsepelaaip nkvstldrrl kmkitrprsl
     1141 dlsnwsvdsh asslytssgs eenislktgk lsrnsstasr ngpyetttpn ssivqsvpea
     1201 ipppnnlkpn gkkinktlqq veqpkrtvlt lmggrgyinw rqlnaqsvpd kasksgytfk
     1261 dpnsndahiv lwemkl
//
LOCUS       XP_034182060            1252 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 10 isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182060
VERSION     XP_034182060.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326169.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1252
                     /product="rho guanine nucleotide exchange factor 10
                     isoform X6"
                     /calculated_mol_wt=139774
     Region          174..355
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          378..522
                     /region_name="PH_19"
                     /note="PH domain; pfam19057"
                     /db_xref="CDD:465965"
     Region          <521..586
                     /region_name="PH"
                     /note="Pleckstrin homology domain; smart00233"
                     /db_xref="CDD:214574"
     Region          649..1010
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1252
                     /gene="LOC117605150"
                     /coded_by="XM_034326169.2:449..4207"
                     /db_xref="GeneID:117605150"
ORIGIN      
        1 metvgqthrq hpsleatrsn tstqsarsed swcsasdhdl ssddeseksn isiksncqlr
       61 ntlhkartlc dkwrsqnmrv nnadpldspg nhgrlsrwfs irrgstqqyd vdssdtvslt
      121 spiktpqmpq lceveeensa ivqfqcmqqr rqtppalppt ppnltpqqlk rrhivaaivh
      181 sensyvstlq rlvndykkpl eessppilsq akiatlfhrl peilqchtlf rialaecvrs
      241 wdkdeklgdv fvasfskaiv ldiysgfinn fsvamdlakq eskrktalad ffkvkqisah
      301 drlsffglmv kpvqrfpqfi lflqdllkht pqghhdrmsl qlaltqlesl aemlnerkre
      361 aeqfqafkem lrhvsgklsh rplssssryl irednvtqle fnqngmitks krrrllllnd
      421 lvvcvsvtpr stedfsgser ltlkwtypvs dieiqdtsts ptlsrlltag lnkggslksd
      481 ksgeggqaga dnlcvemndl mhdyevmsri sdlvaqlkgk yegmtlektk qilqsiqlsi
      541 qqkdedmvwa dscclqlvtk qgqmytfqte nplvkkdwit elrlaqlald pnnspswevp
      601 eqeqrpstkm plfvssqqvy hsqhqtevrc gcyyttqnsr ptrrrgrsqs ylwictgdgi
      661 sshitvfgqs ttasttllkq vtafdlvetr vaaiefikgv ssdplsland lvwmgtdsrk
      721 iiiyaasepe kqeqlgnysv sgpviqikhh cdnvfvalgi gllllfkrqa dgtwnlrdpf
      781 vislgsepvs clmpintsvy aacgkkvwvl hgvngditks fsvqhehvgs vklmahsgvg
      841 lwvalknsst vclyhtetfk hlqdiniasn vlrvtrsnna tnscgdnlnn nqttvtvtal
      901 lackgllwvg tnvgisltip lprlegvpii sgrvnisyha hfgpitflla iqnpkntinc
      961 ptateeinde etvqlrsket ensrstrdra sldssmsnsi iklkqqlags pvmlrrkrsk
     1021 eyeykgsktl prglgsgggf lsssmsgsqs sgencdvygl ygelmyvkdy enenssgidp
     1081 iyeslrrsep elaaipnkvs tldrrlkmki trprsldlsn wsvdshassl ytssgseeni
     1141 slktgklsrn sstasrngpy etttpnssiv qsvpeaippp nnlkpngkki nktlqqveqp
     1201 krtvltlmgg rgyinwrqln aqsvpdkask sgytfkdpns ndahivlwem kl
//
LOCUS       XP_034182073            1347 aa            linear   INV 03-SEP-2025
DEFINITION  tectonin beta-propeller repeat-containing peroxin 23 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034182073
VERSION     XP_034182073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1347
                     /product="tectonin beta-propeller repeat-containing
                     peroxin 23 isoform X1"
                     /calculated_mol_wt=150117
     Region          64..125
                     /region_name="DysFN"
                     /note="Dysferlin domain, N-terminal region; smart00693"
                     /db_xref="CDD:214777"
     Region          199..387
                     /region_name="Tectonin"
                     /note="Tectonin domain; pfam19193"
                     /db_xref="CDD:465992"
     Region          642..760
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          806..943
                     /region_name="GLECT"
                     /note="Galectin/galactose-binding lectin. This domain
                     exclusively binds beta-galactosides, such as lactose, and
                     does not require metal ions for activity. GLECT domains
                     occur as homodimers or tandemly repeated domains. They are
                     developmentally regulated and may...; cd00070"
                     /db_xref="CDD:238025"
     Site            order(807..811,939..943)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            807..811
                     /site_type="active"
                     /note="dimerization swap strand [active]"
                     /db_xref="CDD:238025"
     Site            order(816..818,821,824,902..904,909,911,914)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(860,862,864,871,873,880,883,885)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     Region          1016..1072
                     /region_name="DysFN"
                     /note="Dysferlin domain, N-terminal region; smart00693"
                     /db_xref="CDD:214777"
     Region          <1148..1327
                     /region_name="Tectonin"
                     /note="Tectonin domain; pfam19193"
                     /db_xref="CDD:465992"
     CDS             1..1347
                     /gene="Pex23"
                     /coded_by="XM_034326182.2:1..4044"
                     /db_xref="GeneID:117605176"
ORIGIN      
        1 mpsshlyain negrvfglst tgnmwrefmy lglefkqlsa vphfmwaigg drqvyvhvhg
       61 ldvpirikee iyenerwlpl egfcgrllpt drynfsnqdg tvdrsrdkvk lpsmawqweg
      121 dwqiettldg qpldhdgwty avdfpatyst kkqwkscvrr rkwvryrrys amnswcaiap
      181 lhkdatkepf idisvggnqv pgsssgslav wavtahgrvm frvgtsttcp egqrwsaikl
      241 asgyevcqis vgttglvwav lmigkalvrt gvtrenpmge dwaeidspqk dlkliqvsvg
      301 tdavwavtqd ggvwfrkgik gemsgvceqm atgtgwvemi skmslvsvap ndqvwaighe
      361 drglyyrtgi trseltgkkw riinaplqls rassnaslss nrhsmcgtpq qqrhqswgsl
      421 nrphstsegt tlireweeqs rsaptptslk lwqrtndgts tknpqqtsfq diylnegekk
      481 sansldmadl seasvaniti snesltktge sivvsgkgmg stikinpaaw spvhsvgsmv
      541 gveahpetdg sifdpdltsd sgvygedess gamywaecda swykveagac fvdpanppkw
      601 iadtngtshg diaepwriyi leelkkrlqe iefdpliyek vveksswikn sdakckvkgs
      661 ttyedcviel ewissdsgsl dsgtltilns dgattimqfp vseimcvvcc sepgnpriai
      721 htprlsrskv lrlqfssdte medwlshlts vscqmnnvyg kpgpnsiwat talgdvyvyd
      781 pavvdenqys ngvyvqeldv agkdlpfeci lqngfgygss levtvcihdd adrlsfnlvc
      841 ytttfvkqks vmeshdvalh fnprlkenii vrntyqnghw gdeerngdsp lragsdftlk
      901 ivceprgyri liddteftly shrispqsit hfrikglmtl csvlykstsv iidpitmfwr
      961 qmgghlkkve tcssgvtwgi gydstawvyt ggwgslslkg ldsntgintm vdthnyyvye
     1021 nqrwnpvtgy tshglptdry mwsdatgrhk rtrehtklls mhwhwvsdwi vdfhtpggvd
     1081 rdgwqyatdf psqyhgkkqf tdyvrrrrwf rrcqltasgp wqelgntklt dvslyatdep
     1141 stdapiyiwa vasngealfr rgvsescpmg vswehipsdq alvgiscgpg gqvwavgkng
     1201 ssywrlgits vkpagiqwqn veppsgahlk qisvgkdvvw aldttgrlsv rrevqlnvfp
     1261 egthwqtlpa mpndpihidm svinakqgfr hvsvarkegq vwavsgagil crrigitekn
     1321 pagtgwvtgi ganwqyisvg glvnkak
//
LOCUS       XP_034182081            1176 aa            linear   INV 03-SEP-2025
DEFINITION  tectonin beta-propeller repeat-containing peroxin 23 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034182081
VERSION     XP_034182081.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326190.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1176
                     /product="tectonin beta-propeller repeat-containing
                     peroxin 23 isoform X2"
                     /calculated_mol_wt=129850
     Region          28..216
                     /region_name="Tectonin"
                     /note="Tectonin domain; pfam19193"
                     /db_xref="CDD:465992"
     Region          471..589
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          635..772
                     /region_name="GLECT"
                     /note="Galectin/galactose-binding lectin. This domain
                     exclusively binds beta-galactosides, such as lactose, and
                     does not require metal ions for activity. GLECT domains
                     occur as homodimers or tandemly repeated domains. They are
                     developmentally regulated and may...; cd00070"
                     /db_xref="CDD:238025"
     Site            order(636..640,768..772)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            636..640
                     /site_type="active"
                     /note="dimerization swap strand [active]"
                     /db_xref="CDD:238025"
     Site            order(645..647,650,653,731..733,738,740,743)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(689,691,693,700,702,709,712,714)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     Region          845..901
                     /region_name="DysFN"
                     /note="Dysferlin domain, N-terminal region; smart00693"
                     /db_xref="CDD:214777"
     Region          <977..1156
                     /region_name="Tectonin"
                     /note="Tectonin domain; pfam19193"
                     /db_xref="CDD:465992"
     CDS             1..1176
                     /gene="Pex23"
                     /coded_by="XM_034326190.2:314..3844"
                     /db_xref="GeneID:117605176"
ORIGIN      
        1 mnswcaiapl hkdatkepfi disvggnqvp gsssgslavw avtahgrvmf rvgtsttcpe
       61 gqrwsaikla sgyevcqisv gttglvwavl migkalvrtg vtrenpmged waeidspqkd
      121 lkliqvsvgt davwavtqdg gvwfrkgikg emsgvceqma tgtgwvemis kmslvsvapn
      181 dqvwaighed rglyyrtgit rseltgkkwr iinaplqlsr assnaslssn rhsmcgtpqq
      241 qrhqswgsln rphstsegtt lireweeqsr saptptslkl wqrtndgtst knpqqtsfqd
      301 iylnegekks ansldmadls easvanitis nesltktges ivvsgkgmgs tikinpaaws
      361 pvhsvgsmvg veahpetdgs ifdpdltsds gvygedessg amywaecdas wykveagacf
      421 vdpanppkwi adtngtshgd iaepwriyil eelkkrlqei efdpliyekv veksswikns
      481 dakckvkgst tyedcviele wissdsgsld sgtltilnsd gattimqfpv seimcvvccs
      541 epgnpriaih tprlsrskvl rlqfssdtem edwlshltsv scqmnnvygk pgpnsiwatt
      601 algdvyvydp avvdenqysn gvyvqeldva gkdlpfecil qngfgygssl evtvcihdda
      661 drlsfnlvcy tttfvkqksv meshdvalhf nprlkeniiv rntyqnghwg deerngdspl
      721 ragsdftlki vceprgyril iddteftlys hrispqsith frikglmtlc svlykstsvi
      781 idpitmfwrq mgghlkkvet cssgvtwgig ydstawvytg gwgslslkgl dsntgintmv
      841 dthnyyvyen qrwnpvtgyt shglptdrym wsdatgrhkr trehtkllsm hwhwvsdwiv
      901 dfhtpggvdr dgwqyatdfp sqyhgkkqft dyvrrrrwfr rcqltasgpw qelgntkltd
      961 vslyatdeps tdapiyiwav asngealfrr gvsescpmgv swehipsdqa lvgiscgpgg
     1021 qvwavgkngs sywrlgitsv kpagiqwqnv eppsgahlkq isvgkdvvwa ldttgrlsvr
     1081 revqlnvfpe gthwqtlpam pndpihidms vinakqgfrh vsvarkegqv wavsgagilc
     1141 rrigiteknp agtgwvtgig anwqyisvgg lvnkak
//
LOCUS       XP_034182126            1154 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182126
VERSION     XP_034182126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1154
                     /product="chromodomain-containing protein chromator
                     isoform X1"
                     /calculated_mol_wt=123403
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     Region          <560..>714
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..1154
                     /gene="Chro"
                     /coded_by="XM_034326235.2:204..3668"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggtq ktepikiqpk pdptqlpqih vvtavpapit lqprlstgir pgpvtgqrga
      601 dgrpllprpp lrattptsvl gigstirtpv rapaprqvqs qqarqvlqkr ttavttqsns
      661 vspgnvtqir pkftvlspqp kqviksgtsp vqqvkqppkt pvgkpqslls pqqkllmakr
      721 kaqeaagiik pggrgllaga ratagrgrgk laetptaatv pgskqkeskl aegdglhmef
      781 hevgseesss egepelpppe adtiattepd spprpftlcp ltgriigpdg epveqpaepe
      841 pepepttpls tvktttttse sidvtatatt telvlpslds lteaggimrv emspggttgt
      901 ivqtsepaqi nltnvsvpap dlpclddtap avatpvttta tplteatsss essiaagltt
      961 atvtststia iastdvtkse eklseerkva edasnlvtit gedgvvyqva gqaedgqtll
     1021 vtrdadgeqq cvyvtteqqg degsvltldh avaeavaqli pdqvnlasqf yvkeggtept
     1081 enpmvmsimd ntnatdvteg qeegdghgqv vaqvvqaeep tpggtrrvvl llpdgnlmmt
     1141 evteeqyaal glgk
//
LOCUS       XP_034182095            1154 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182095
VERSION     XP_034182095.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326204.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1154
                     /product="chromodomain-containing protein chromator
                     isoform X1"
                     /calculated_mol_wt=123403
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     Region          <560..>714
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..1154
                     /gene="Chro"
                     /coded_by="XM_034326204.2:179..3643"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggtq ktepikiqpk pdptqlpqih vvtavpapit lqprlstgir pgpvtgqrga
      601 dgrpllprpp lrattptsvl gigstirtpv rapaprqvqs qqarqvlqkr ttavttqsns
      661 vspgnvtqir pkftvlspqp kqviksgtsp vqqvkqppkt pvgkpqslls pqqkllmakr
      721 kaqeaagiik pggrgllaga ratagrgrgk laetptaatv pgskqkeskl aegdglhmef
      781 hevgseesss egepelpppe adtiattepd spprpftlcp ltgriigpdg epveqpaepe
      841 pepepttpls tvktttttse sidvtatatt telvlpslds lteaggimrv emspggttgt
      901 ivqtsepaqi nltnvsvpap dlpclddtap avatpvttta tplteatsss essiaagltt
      961 atvtststia iastdvtkse eklseerkva edasnlvtit gedgvvyqva gqaedgqtll
     1021 vtrdadgeqq cvyvtteqqg degsvltldh avaeavaqli pdqvnlasqf yvkeggtept
     1081 enpmvmsimd ntnatdvteg qeegdghgqv vaqvvqaeep tpggtrrvvl llpdgnlmmt
     1141 evteeqyaal glgk
//
LOCUS       XP_034182101            1154 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182101
VERSION     XP_034182101.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326210.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1154
                     /product="chromodomain-containing protein chromator
                     isoform X1"
                     /calculated_mol_wt=123403
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     Region          <560..>714
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..1154
                     /gene="Chro"
                     /coded_by="XM_034326210.2:187..3651"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggtq ktepikiqpk pdptqlpqih vvtavpapit lqprlstgir pgpvtgqrga
      601 dgrpllprpp lrattptsvl gigstirtpv rapaprqvqs qqarqvlqkr ttavttqsns
      661 vspgnvtqir pkftvlspqp kqviksgtsp vqqvkqppkt pvgkpqslls pqqkllmakr
      721 kaqeaagiik pggrgllaga ratagrgrgk laetptaatv pgskqkeskl aegdglhmef
      781 hevgseesss egepelpppe adtiattepd spprpftlcp ltgriigpdg epveqpaepe
      841 pepepttpls tvktttttse sidvtatatt telvlpslds lteaggimrv emspggttgt
      901 ivqtsepaqi nltnvsvpap dlpclddtap avatpvttta tplteatsss essiaagltt
      961 atvtststia iastdvtkse eklseerkva edasnlvtit gedgvvyqva gqaedgqtll
     1021 vtrdadgeqq cvyvtteqqg degsvltldh avaeavaqli pdqvnlasqf yvkeggtept
     1081 enpmvmsimd ntnatdvteg qeegdghgqv vaqvvqaeep tpggtrrvvl llpdgnlmmt
     1141 evteeqyaal glgk
//
LOCUS       XP_034182110            1154 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182110
VERSION     XP_034182110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1154
                     /product="chromodomain-containing protein chromator
                     isoform X1"
                     /calculated_mol_wt=123403
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     Region          <560..>714
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..1154
                     /gene="Chro"
                     /coded_by="XM_034326219.2:199..3663"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggtq ktepikiqpk pdptqlpqih vvtavpapit lqprlstgir pgpvtgqrga
      601 dgrpllprpp lrattptsvl gigstirtpv rapaprqvqs qqarqvlqkr ttavttqsns
      661 vspgnvtqir pkftvlspqp kqviksgtsp vqqvkqppkt pvgkpqslls pqqkllmakr
      721 kaqeaagiik pggrgllaga ratagrgrgk laetptaatv pgskqkeskl aegdglhmef
      781 hevgseesss egepelpppe adtiattepd spprpftlcp ltgriigpdg epveqpaepe
      841 pepepttpls tvktttttse sidvtatatt telvlpslds lteaggimrv emspggttgt
      901 ivqtsepaqi nltnvsvpap dlpclddtap avatpvttta tplteatsss essiaagltt
      961 atvtststia iastdvtkse eklseerkva edasnlvtit gedgvvyqva gqaedgqtll
     1021 vtrdadgeqq cvyvtteqqg degsvltldh avaeavaqli pdqvnlasqf yvkeggtept
     1081 enpmvmsimd ntnatdvteg qeegdghgqv vaqvvqaeep tpggtrrvvl llpdgnlmmt
     1141 evteeqyaal glgk
//
LOCUS       XP_034182115            1154 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182115
VERSION     XP_034182115.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326224.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1154
                     /product="chromodomain-containing protein chromator
                     isoform X1"
                     /calculated_mol_wt=123403
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     Region          <560..>714
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..1154
                     /gene="Chro"
                     /coded_by="XM_034326224.2:161..3625"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggtq ktepikiqpk pdptqlpqih vvtavpapit lqprlstgir pgpvtgqrga
      601 dgrpllprpp lrattptsvl gigstirtpv rapaprqvqs qqarqvlqkr ttavttqsns
      661 vspgnvtqir pkftvlspqp kqviksgtsp vqqvkqppkt pvgkpqslls pqqkllmakr
      721 kaqeaagiik pggrgllaga ratagrgrgk laetptaatv pgskqkeskl aegdglhmef
      781 hevgseesss egepelpppe adtiattepd spprpftlcp ltgriigpdg epveqpaepe
      841 pepepttpls tvktttttse sidvtatatt telvlpslds lteaggimrv emspggttgt
      901 ivqtsepaqi nltnvsvpap dlpclddtap avatpvttta tplteatsss essiaagltt
      961 atvtststia iastdvtkse eklseerkva edasnlvtit gedgvvyqva gqaedgqtll
     1021 vtrdadgeqq cvyvtteqqg degsvltldh avaeavaqli pdqvnlasqf yvkeggtept
     1081 enpmvmsimd ntnatdvteg qeegdghgqv vaqvvqaeep tpggtrrvvl llpdgnlmmt
     1141 evteeqyaal glgk
//
LOCUS       XP_076546341            1132 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546341
VERSION     XP_076546341.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690226.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1132
                     /product="chromodomain-containing protein chromator
                     isoform X2"
                     /calculated_mol_wt=121171
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     Region          <560..>714
                     /region_name="PHA03379"
                     /note="EBNA-3A; Provisional"
                     /db_xref="CDD:223066"
     CDS             1..1132
                     /gene="Chro"
                     /coded_by="XM_076690226.1:204..3602"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggtq ktepikiqpk pdptqlpqih vvtavpapit lqprlstgir pgpvtgqrga
      601 dgrpllprpp lrattptsvl gigstirtpv rapaprqvqs qqarqvlqkr ttavttqsns
      661 vspgnvtqir pkftvlspqp kqviksgtsp vqqvkqppkt pvgkpqslls pqqkllmakr
      721 kaqeaagiik pggrgllaga ratagrgrgk laetptaatv pgskqkeskl aegdglhmef
      781 hevgseesss egepelpppe adtiattepd spprpftlcp ltgriigpdg epveqpaepe
      841 pepepttpls tvktttttse kaggimrvem spggttgtiv qtsepaqinl tnvsvpapdl
      901 pclddtapav atpvtttatp lteatssses siaaglttat vtststiaia stdvtkseek
      961 lseerkvaed asnlvtitge dgvvyqvagq aedgqtllvt rdadgeqqcv yvtteqqgde
     1021 gsvltldhav aeavaqlipd qvnlasqfyv keggtepten pmvmsimdnt natdvtegqe
     1081 egdghgqvva qvvqaeeptp ggtrrvvlll pdgnlmmtev teeqyaalgl gk
//
LOCUS       XP_034182134            1125 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182134
VERSION     XP_034182134.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326243.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1125
                     /product="chromodomain-containing protein chromator
                     isoform X3"
                     /calculated_mol_wt=120290
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     CDS             1..1125
                     /gene="Chro"
                     /coded_by="XM_034326243.2:204..3581"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggtq ktepikiqpk pdptqlpqih vvtavpapit lqprlstgir pgpvtgqrga
      601 dgrpllprpp lrattptsvl gigstirtpv rapaprqvqs qqarqvlqkr ttavttqsns
      661 vspgnvtqir pkftvlspqp kqviksgtsp vqqvkqppkt pvggrgllag aratagrgrg
      721 klaetptaat vpgskqkesk laegdglhme fhevgseess segepelppp eadtiattep
      781 dspprpftlc pltgriigpd gepveqpaep epepepttpl stvkttttts esidvtatat
      841 ttelvlpsld slteaggimr vemspggttg tivqtsepaq inltnvsvpa pdlpclddta
      901 pavatpvttt atplteatss sessiaaglt tatvtststi aiastdvtks eeklseerkv
      961 aedasnlvti tgedgvvyqv agqaedgqtl lvtrdadgeq qcvyvtteqq gdegsvltld
     1021 havaeavaql ipdqvnlasq fyvkeggtep tenpmvmsim dntnatdvte gqeegdghgq
     1081 vvaqvvqaee ptpggtrrvv lllpdgnlmm tevteeqyaa lglgk
//
LOCUS       XP_034182142            1106 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain-containing protein chromator isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182142
VERSION     XP_034182142.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326251.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1106
                     /product="chromodomain-containing protein chromator
                     isoform X4"
                     /calculated_mol_wt=118303
     Region          219..270
                     /region_name="CD_CSD"
                     /note="CHROMO (CHRromatin Organization Modifier) domains
                     and chromo shadow domains; cl28914"
                     /db_xref="CDD:475127"
     Site            order(219..223,240,242..245,249,251..254,256..257,264,
                     267..268)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349274"
     CDS             1..1106
                     /gene="Chro"
                     /coded_by="XM_034326251.2:204..3524"
                     /db_xref="GeneID:117605188"
ORIGIN      
        1 meggdgpsai ssnptaikaa qeemgrldvl vcgqchsvfh fieefqehrt kegactqvsh
       61 frensnneqk aqvwafllwk dsqiqqegsd kdstnswkly qkwckmdthi rdswiaagkt
      121 iqtftkisna kmqdaprqnq istnaegksv vvrkvirngq pedaerkdgk asetrnqkne
      181 sfdtevekke kpklkpsikp kgksskagee drdssdeeyt vekilakrfn pkkrcseyll
      241 kwegyghehn twepaehvat ckhlleefer nlakqkelka aqqqanskaa grgshlahkt
      301 vikaeakpgp stavhvgrpm rsskskamdq vkqwcgsmkd edndllgkrr mdysesdsed
      361 ggssaakrak gdtgsdedwt geseeerllg rsdviqrafn ranaqsngsn rasgsssdla
      421 tslglqspeg aksnqppvlv anakgvvkvd pkqmpnltsg vyvmsrkdgi ikldsspsgk
      481 lsvkgspttq gvlmvqnrdn tnvvrkqvis asqsnsvtpv kvvskmdgsq vvtqmkvvsk
      541 pvttktggqr gadgrpllpr pplrattpts vlgigstirt pvrapaprqv qsqqarqvlq
      601 krttavttqs nsvspgnvtq irpkftvlsp qpkqviksgt spvqqvkqpp ktpvgkpqsl
      661 lspqqkllma krkaqeaagi ikpggrglla garatagrgr gklaetptaa tvpgskqkes
      721 klaegdglhm efhevgsees ssegepelpp peadtiatte pdspprpftl cpltgriigp
      781 dgepveqpae pepepepttp lstvkttttt sesidvtata tttelvlpsl dslteaggim
      841 rvemspggtt gtivqtsepa qinltnvsvp apdlpclddt apavatpvtt tatplteats
      901 ssessiaagl ttatvtstst iaiastdvtk seeklseerk vaedasnlvt itgedgvvyq
      961 vagqaedgqt llvtrdadge qqcvyvtteq qgdegsvltl dhavaeavaq lipdqvnlas
     1021 qfyvkeggte ptenpmvmsi mdntnatdvt egqeegdghg qvvaqvvqae eptpggtrrv
     1081 vlllpdgnlm mtevteeqya alglgk
//
LOCUS       XP_034182154             970 aa            linear   INV 03-SEP-2025
DEFINITION  chordin short gastrulation isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034182154
VERSION     XP_034182154.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326263.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..970
                     /product="chordin short gastrulation isoform X1"
                     /calculated_mol_wt=106691
     Region          44..116
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     Region          179..274
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          412..517
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          541..630
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          686..745
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          766..832
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          882..>922
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     CDS             1..970
                     /gene="sog"
                     /coded_by="XM_034326263.2:2424..5336"
                     /db_xref="GeneID:117605216"
ORIGIN      
        1 mhfhggllsi llivvscplt lrarrvapli eddwtrrphr aaectfgkqi relgstwfad
       61 lgppfgvmyc ikcecipvqk krrivarvhc rniknecpkl tcdepvllpg rcckscpgdf
      121 stdivqdlpa qltseeeers lklltfsdfg tlltgrtsqv lrrdepspts iynsaynyva
      181 tgrftfhrkn lyysfylsta tprprsiqfv dgsgsileeq ivdpiggvyq natgklcgvw
      241 rrvprdyrkl lreerlhvsf iwepsatltg qlsryralst eqyssllept mgvdrslmsg
      301 agataivsas sasapsihvs lvfngvflpn dvlevplivq lehvekdyvv lreeiivkkp
      361 snelnfgevr salsgadlrl ltrgklsisi mskkdpqair lagmvgprat cdmyqtllls
      421 etpsaasgla wafldragsl rygvqligle eesplvtlvd eggkrrtele dltpslvdgl
      481 angsldrpgp rlleplfnge lsvvaashvg smlrgrlyqr pvadardtta pvllrrlsqe
      541 pvhpgpvglv wvavdldcsl hyeleiagfp qpsptsdtep gtfrlyletm pllaqgapva
      601 rrlleeftgy vlegsvtgls pvelyriesg igflevtdkq aerilkapfk araplnclph
      661 yadndvasvv aynlqpprsd ietgacfhet rfyeegtqwt sstdpcsmch cfrglaqcdp
      721 vpcptltcpq gkqlkpatgh ccpicagpgi nvngtgknvs svtrgctlag qfhlpgaswh
      781 pylppagfdt cavctcdpvt levkcprvqc ppldcdekia frpskkaccr qcptttpsta
      841 nagvaagdtt rlprdqavps trrtpeeila sggckyplgg pyengmewhp rvhshgemkc
      901 vkcrckngev rcdrkrcpra lcnsishqmk rgeyvgevdd cctvqcrrar rhhrnnhhpa
      961 rhsvtprels
//
LOCUS       XP_034182164             965 aa            linear   INV 03-SEP-2025
DEFINITION  chordin short gastrulation isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182164
VERSION     XP_034182164.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326273.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..965
                     /product="chordin short gastrulation isoform X2"
                     /calculated_mol_wt=106151
     Region          44..116
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     Region          143..269
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          407..512
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          536..625
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          681..740
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          761..827
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          877..>917
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     CDS             1..965
                     /gene="sog"
                     /coded_by="XM_034326273.2:2423..5320"
                     /db_xref="GeneID:117605216"
ORIGIN      
        1 mhfhggllsi llivvscplt lrarrvapli eddwtrrphr aaectfgkqi relgstwfad
       61 lgppfgvmyc ikcecipvqk krrivarvhc rniknecpkl tcdepvllpg rcckscpgdf
      121 stdivqdlpa qltseeeers lkhfgtlltg rtsqvlrrde psptsiynsa ynyvatgrft
      181 fhrknlyysf ylstatprpr siqfvdgsgs ileeqivdpi ggvyqnatgk lcgvwrrvpr
      241 dyrkllreer lhvsfiweps atltgqlsry ralsteqyss lleptmgvdr slmsgagata
      301 ivsassasap sihvslvfng vflpndvlev plivqlehve kdyvvlreei ivkkpsneln
      361 fgevrsalsg adlrlltrgk lsisimskkd pqairlagmv gpratcdmyq tlllsetpsa
      421 asglawafld ragslrygvq ligleeespl vtlvdeggkr rteledltps lvdglangsl
      481 drpgprllep lfngelsvva ashvgsmlrg rlyqrpvada rdttapvllr rlsqepvhpg
      541 pvglvwvavd ldcslhyele iagfpqpspt sdtepgtfrl yletmpllaq gapvarrlle
      601 eftgyvlegs vtglspvely riesgigfle vtdkqaeril kapfkarapl nclphyadnd
      661 vasvvaynlq pprsdietga cfhetrfyee gtqwtsstdp csmchcfrgl aqcdpvpcpt
      721 ltcpqgkqlk patghccpic agpginvngt gknvssvtrg ctlagqfhlp gaswhpylpp
      781 agfdtcavct cdpvtlevkc prvqcppldc dekiafrpsk kaccrqcptt tpstanagva
      841 agdttrlprd qavpstrrtp eeilasggck yplggpyeng mewhprvhsh gemkcvkcrc
      901 kngevrcdrk rcpralcnsi shqmkrgeyv gevddcctvq crrarrhhrn nhhparhsvt
      961 prels
//
LOCUS       XP_076546342             903 aa            linear   INV 03-SEP-2025
DEFINITION  chordin short gastrulation isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546342
VERSION     XP_076546342.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690227.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..903
                     /product="chordin short gastrulation isoform X3"
                     /calculated_mol_wt=99182
     Region          112..207
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          345..450
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          474..563
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          619..678
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          699..765
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          815..>855
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     CDS             1..903
                     /gene="sog"
                     /coded_by="XM_076690227.1:2900..5611"
                     /db_xref="GeneID:117605216"
ORIGIN      
        1 mycikcecip vqkkrrivar vhcrniknec pkltcdepvl lpgrcckscp gdfstdivqd
       61 lpaqltseee erslklltfs dfgtlltgrt sqvlrrdeps ptsiynsayn yvatgrftfh
      121 rknlyysfyl statprprsi qfvdgsgsil eeqivdpigg vyqnatgklc gvwrrvprdy
      181 rkllreerlh vsfiwepsat ltgqlsryra lsteqyssll eptmgvdrsl msgagataiv
      241 sassasapsi hvslvfngvf lpndvlevpl ivqlehvekd yvvlreeiiv kkpsnelnfg
      301 evrsalsgad lrlltrgkls isimskkdpq airlagmvgp ratcdmyqtl llsetpsaas
      361 glawafldra gslrygvqli gleeesplvt lvdeggkrrt eledltpslv dglangsldr
      421 pgprlleplf ngelsvvaas hvgsmlrgrl yqrpvadard ttapvllrrl sqepvhpgpv
      481 glvwvavdld cslhyeleia gfpqpsptsd tepgtfrlyl etmpllaqga pvarrlleef
      541 tgyvlegsvt glspvelyri esgigflevt dkqaerilka pfkaraplnc lphyadndva
      601 svvaynlqpp rsdietgacf hetrfyeegt qwtsstdpcs mchcfrglaq cdpvpcptlt
      661 cpqgkqlkpa tghccpicag pginvngtgk nvssvtrgct lagqfhlpga swhpylppag
      721 fdtcavctcd pvtlevkcpr vqcppldcde kiafrpskka ccrqcptttp stanagvaag
      781 dttrlprdqa vpstrrtpee ilasggckyp lggpyengme whprvhshge mkcvkcrckn
      841 gevrcdrkrc pralcnsish qmkrgeyvge vddcctvqcr rarrhhrnnh hparhsvtpr
      901 els
//
LOCUS       XP_076546343             903 aa            linear   INV 03-SEP-2025
DEFINITION  chordin short gastrulation isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546343
VERSION     XP_076546343.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690228.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..903
                     /product="chordin short gastrulation isoform X3"
                     /calculated_mol_wt=99182
     Region          112..207
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          345..450
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          474..563
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          619..678
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          699..765
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          815..>855
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     CDS             1..903
                     /gene="sog"
                     /coded_by="XM_076690228.1:2755..5466"
                     /db_xref="GeneID:117605216"
ORIGIN      
        1 mycikcecip vqkkrrivar vhcrniknec pkltcdepvl lpgrcckscp gdfstdivqd
       61 lpaqltseee erslklltfs dfgtlltgrt sqvlrrdeps ptsiynsayn yvatgrftfh
      121 rknlyysfyl statprprsi qfvdgsgsil eeqivdpigg vyqnatgklc gvwrrvprdy
      181 rkllreerlh vsfiwepsat ltgqlsryra lsteqyssll eptmgvdrsl msgagataiv
      241 sassasapsi hvslvfngvf lpndvlevpl ivqlehvekd yvvlreeiiv kkpsnelnfg
      301 evrsalsgad lrlltrgkls isimskkdpq airlagmvgp ratcdmyqtl llsetpsaas
      361 glawafldra gslrygvqli gleeesplvt lvdeggkrrt eledltpslv dglangsldr
      421 pgprlleplf ngelsvvaas hvgsmlrgrl yqrpvadard ttapvllrrl sqepvhpgpv
      481 glvwvavdld cslhyeleia gfpqpsptsd tepgtfrlyl etmpllaqga pvarrlleef
      541 tgyvlegsvt glspvelyri esgigflevt dkqaerilka pfkaraplnc lphyadndva
      601 svvaynlqpp rsdietgacf hetrfyeegt qwtsstdpcs mchcfrglaq cdpvpcptlt
      661 cpqgkqlkpa tghccpicag pginvngtgk nvssvtrgct lagqfhlpga swhpylppag
      721 fdtcavctcd pvtlevkcpr vqcppldcde kiafrpskka ccrqcptttp stanagvaag
      781 dttrlprdqa vpstrrtpee ilasggckyp lggpyengme whprvhshge mkcvkcrckn
      841 gevrcdrkrc pralcnsish qmkrgeyvge vddcctvqcr rarrhhrnnh hparhsvtpr
      901 els
//
LOCUS       XP_076546344             903 aa            linear   INV 03-SEP-2025
DEFINITION  chordin short gastrulation isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546344
VERSION     XP_076546344.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690229.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..903
                     /product="chordin short gastrulation isoform X3"
                     /calculated_mol_wt=99182
     Region          112..207
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          345..450
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          474..563
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          619..678
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          699..765
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          815..>855
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     CDS             1..903
                     /gene="sog"
                     /coded_by="XM_076690229.1:2680..5391"
                     /db_xref="GeneID:117605216"
ORIGIN      
        1 mycikcecip vqkkrrivar vhcrniknec pkltcdepvl lpgrcckscp gdfstdivqd
       61 lpaqltseee erslklltfs dfgtlltgrt sqvlrrdeps ptsiynsayn yvatgrftfh
      121 rknlyysfyl statprprsi qfvdgsgsil eeqivdpigg vyqnatgklc gvwrrvprdy
      181 rkllreerlh vsfiwepsat ltgqlsryra lsteqyssll eptmgvdrsl msgagataiv
      241 sassasapsi hvslvfngvf lpndvlevpl ivqlehvekd yvvlreeiiv kkpsnelnfg
      301 evrsalsgad lrlltrgkls isimskkdpq airlagmvgp ratcdmyqtl llsetpsaas
      361 glawafldra gslrygvqli gleeesplvt lvdeggkrrt eledltpslv dglangsldr
      421 pgprlleplf ngelsvvaas hvgsmlrgrl yqrpvadard ttapvllrrl sqepvhpgpv
      481 glvwvavdld cslhyeleia gfpqpsptsd tepgtfrlyl etmpllaqga pvarrlleef
      541 tgyvlegsvt glspvelyri esgigflevt dkqaerilka pfkaraplnc lphyadndva
      601 svvaynlqpp rsdietgacf hetrfyeegt qwtsstdpcs mchcfrglaq cdpvpcptlt
      661 cpqgkqlkpa tghccpicag pginvngtgk nvssvtrgct lagqfhlpga swhpylppag
      721 fdtcavctcd pvtlevkcpr vqcppldcde kiafrpskka ccrqcptttp stanagvaag
      781 dttrlprdqa vpstrrtpee ilasggckyp lggpyengme whprvhshge mkcvkcrckn
      841 gevrcdrkrc pralcnsish qmkrgeyvge vddcctvqcr rarrhhrnnh hparhsvtpr
      901 els
//
LOCUS       XP_076546345             903 aa            linear   INV 03-SEP-2025
DEFINITION  chordin short gastrulation isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546345
VERSION     XP_076546345.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690230.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..903
                     /product="chordin short gastrulation isoform X3"
                     /calculated_mol_wt=99182
     Region          112..207
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          345..450
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          474..563
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          619..678
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          699..765
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          815..>855
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     CDS             1..903
                     /gene="sog"
                     /coded_by="XM_076690230.1:2895..5606"
                     /db_xref="GeneID:117605216"
ORIGIN      
        1 mycikcecip vqkkrrivar vhcrniknec pkltcdepvl lpgrcckscp gdfstdivqd
       61 lpaqltseee erslklltfs dfgtlltgrt sqvlrrdeps ptsiynsayn yvatgrftfh
      121 rknlyysfyl statprprsi qfvdgsgsil eeqivdpigg vyqnatgklc gvwrrvprdy
      181 rkllreerlh vsfiwepsat ltgqlsryra lsteqyssll eptmgvdrsl msgagataiv
      241 sassasapsi hvslvfngvf lpndvlevpl ivqlehvekd yvvlreeiiv kkpsnelnfg
      301 evrsalsgad lrlltrgkls isimskkdpq airlagmvgp ratcdmyqtl llsetpsaas
      361 glawafldra gslrygvqli gleeesplvt lvdeggkrrt eledltpslv dglangsldr
      421 pgprlleplf ngelsvvaas hvgsmlrgrl yqrpvadard ttapvllrrl sqepvhpgpv
      481 glvwvavdld cslhyeleia gfpqpsptsd tepgtfrlyl etmpllaqga pvarrlleef
      541 tgyvlegsvt glspvelyri esgigflevt dkqaerilka pfkaraplnc lphyadndva
      601 svvaynlqpp rsdietgacf hetrfyeegt qwtsstdpcs mchcfrglaq cdpvpcptlt
      661 cpqgkqlkpa tghccpicag pginvngtgk nvssvtrgct lagqfhlpga swhpylppag
      721 fdtcavctcd pvtlevkcpr vqcppldcde kiafrpskka ccrqcptttp stanagvaag
      781 dttrlprdqa vpstrrtpee ilasggckyp lggpyengme whprvhshge mkcvkcrckn
      841 gevrcdrkrc pralcnsish qmkrgeyvge vddcctvqcr rarrhhrnnh hparhsvtpr
      901 els
//
LOCUS       XP_076546346             903 aa            linear   INV 03-SEP-2025
DEFINITION  chordin short gastrulation isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546346
VERSION     XP_076546346.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690231.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..903
                     /product="chordin short gastrulation isoform X3"
                     /calculated_mol_wt=99182
     Region          112..207
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          345..450
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          474..563
                     /region_name="CHRD"
                     /note="CHRD domain; cl06473"
                     /db_xref="CDD:471449"
     Region          619..678
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          699..765
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; pfam00093"
                     /db_xref="CDD:278520"
     Region          815..>855
                     /region_name="VWC"
                     /note="von Willebrand factor type C domain; cl17735"
                     /db_xref="CDD:450195"
     CDS             1..903
                     /gene="sog"
                     /coded_by="XM_076690231.1:2750..5461"
                     /db_xref="GeneID:117605216"
ORIGIN      
        1 mycikcecip vqkkrrivar vhcrniknec pkltcdepvl lpgrcckscp gdfstdivqd
       61 lpaqltseee erslklltfs dfgtlltgrt sqvlrrdeps ptsiynsayn yvatgrftfh
      121 rknlyysfyl statprprsi qfvdgsgsil eeqivdpigg vyqnatgklc gvwrrvprdy
      181 rkllreerlh vsfiwepsat ltgqlsryra lsteqyssll eptmgvdrsl msgagataiv
      241 sassasapsi hvslvfngvf lpndvlevpl ivqlehvekd yvvlreeiiv kkpsnelnfg
      301 evrsalsgad lrlltrgkls isimskkdpq airlagmvgp ratcdmyqtl llsetpsaas
      361 glawafldra gslrygvqli gleeesplvt lvdeggkrrt eledltpslv dglangsldr
      421 pgprlleplf ngelsvvaas hvgsmlrgrl yqrpvadard ttapvllrrl sqepvhpgpv
      481 glvwvavdld cslhyeleia gfpqpsptsd tepgtfrlyl etmpllaqga pvarrlleef
      541 tgyvlegsvt glspvelyri esgigflevt dkqaerilka pfkaraplnc lphyadndva
      601 svvaynlqpp rsdietgacf hetrfyeegt qwtsstdpcs mchcfrglaq cdpvpcptlt
      661 cpqgkqlkpa tghccpicag pginvngtgk nvssvtrgct lagqfhlpga swhpylppag
      721 fdtcavctcd pvtlevkcpr vqcppldcde kiafrpskka ccrqcptttp stanagvaag
      781 dttrlprdqa vpstrrtpee ilasggckyp lggpyengme whprvhshge mkcvkcrckn
      841 gevrcdrkrc pralcnsish qmkrgeyvge vddcctvqcr rarrhhrnnh hparhsvtpr
      901 els
//
LOCUS       XP_034182232             794 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylneuraminate (7)9-O-acetyltransferase [Osmia lignaria
            lignaria].
ACCESSION   XP_034182232
VERSION     XP_034182232.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326341.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182232.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..794
                     /product="N-acetylneuraminate (7)9-O-acetyltransferase"
                     /calculated_mol_wt=90937
     Region          294..778
                     /region_name="Cas1_AcylT"
                     /note="10 TM Acyl Transferase domain found in Cas1p;
                     pfam07779"
                     /db_xref="CDD:462263"
     CDS             1..794
                     /gene="LOC117605241"
                     /coded_by="XM_034326341.2:373..2757"
                     /db_xref="GeneID:117605241"
ORIGIN      
        1 mtsaehfisl itvasakkla tglvlcfily hglmhliygs dsckwllteg rykgdkewqp
       61 ygcmmhyytq tdsrrclryl afmghrnhfv ftgdarirql yksfisqfiv dgktsdltel
      121 ldnsdlhfnd aqlrlnvqfl wrpqldnami edlrswmknd apsliiagca amtilannns
      181 daqlyseytm glvrlvqpvd flskkntkyl wmlqdpvlke rlptqlsgid nrqinlcnka
      241 avkilshsea hiwessklvg tgvleqspdg ylasplslrh kvqlllntyc ndhmnfddgs
      301 ccsypepatt lqllslsala lcivigggiw iyrkfcyyrt eisylrvtta eventeeant
      361 seitqieqpe aqdfytlits lallsiilay fylcdrtnff mkenkyysef sfwlplgyil
      421 alglfftedr ergpralnre qtdewrglmq avvliyhvtg aknvlpiymy lrlinsaylf
      481 lsgyghfcyf wqtgdvslvr farvmfrlnf ltvslclcmn rpyqfyhfvp lvsfwflviy
      541 flawippriy sgslneygpr allylalkll gliwmitily msevffekvf vtrpwkalfv
      601 ttdddireww srwrvdrysv awgvtfgagl valqridhip gtalssvlal isltayttft
      661 ilchsvsece eihsyvafip iigyialrna slalrgkhsa llaglgrisl etlvaqghiw
      721 laadshgvlv llprfpvlnl lvtsfifica sheihrltrv lapyavpndw rlvarnlllf
      781 iavlvpigih dgmf
//
LOCUS       XP_034182239             794 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylneuraminate (7)9-O-acetyltransferase [Osmia lignaria
            lignaria].
ACCESSION   XP_034182239
VERSION     XP_034182239.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326348.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182239.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..794
                     /product="N-acetylneuraminate (7)9-O-acetyltransferase"
                     /calculated_mol_wt=90937
     Region          294..778
                     /region_name="Cas1_AcylT"
                     /note="10 TM Acyl Transferase domain found in Cas1p;
                     pfam07779"
                     /db_xref="CDD:462263"
     CDS             1..794
                     /gene="LOC117605241"
                     /coded_by="XM_034326348.2:169..2553"
                     /db_xref="GeneID:117605241"
ORIGIN      
        1 mtsaehfisl itvasakkla tglvlcfily hglmhliygs dsckwllteg rykgdkewqp
       61 ygcmmhyytq tdsrrclryl afmghrnhfv ftgdarirql yksfisqfiv dgktsdltel
      121 ldnsdlhfnd aqlrlnvqfl wrpqldnami edlrswmknd apsliiagca amtilannns
      181 daqlyseytm glvrlvqpvd flskkntkyl wmlqdpvlke rlptqlsgid nrqinlcnka
      241 avkilshsea hiwessklvg tgvleqspdg ylasplslrh kvqlllntyc ndhmnfddgs
      301 ccsypepatt lqllslsala lcivigggiw iyrkfcyyrt eisylrvtta eventeeant
      361 seitqieqpe aqdfytlits lallsiilay fylcdrtnff mkenkyysef sfwlplgyil
      421 alglfftedr ergpralnre qtdewrglmq avvliyhvtg aknvlpiymy lrlinsaylf
      481 lsgyghfcyf wqtgdvslvr farvmfrlnf ltvslclcmn rpyqfyhfvp lvsfwflviy
      541 flawippriy sgslneygpr allylalkll gliwmitily msevffekvf vtrpwkalfv
      601 ttdddireww srwrvdrysv awgvtfgagl valqridhip gtalssvlal isltayttft
      661 ilchsvsece eihsyvafip iigyialrna slalrgkhsa llaglgrisl etlvaqghiw
      721 laadshgvlv llprfpvlnl lvtsfifica sheihrltrv lapyavpndw rlvarnlllf
      781 iavlvpigih dgmf
//
LOCUS       XP_034182249             794 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylneuraminate (7)9-O-acetyltransferase [Osmia lignaria
            lignaria].
ACCESSION   XP_034182249
VERSION     XP_034182249.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326358.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182249.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..794
                     /product="N-acetylneuraminate (7)9-O-acetyltransferase"
                     /calculated_mol_wt=90937
     Region          294..778
                     /region_name="Cas1_AcylT"
                     /note="10 TM Acyl Transferase domain found in Cas1p;
                     pfam07779"
                     /db_xref="CDD:462263"
     CDS             1..794
                     /gene="LOC117605241"
                     /coded_by="XM_034326358.2:170..2554"
                     /db_xref="GeneID:117605241"
ORIGIN      
        1 mtsaehfisl itvasakkla tglvlcfily hglmhliygs dsckwllteg rykgdkewqp
       61 ygcmmhyytq tdsrrclryl afmghrnhfv ftgdarirql yksfisqfiv dgktsdltel
      121 ldnsdlhfnd aqlrlnvqfl wrpqldnami edlrswmknd apsliiagca amtilannns
      181 daqlyseytm glvrlvqpvd flskkntkyl wmlqdpvlke rlptqlsgid nrqinlcnka
      241 avkilshsea hiwessklvg tgvleqspdg ylasplslrh kvqlllntyc ndhmnfddgs
      301 ccsypepatt lqllslsala lcivigggiw iyrkfcyyrt eisylrvtta eventeeant
      361 seitqieqpe aqdfytlits lallsiilay fylcdrtnff mkenkyysef sfwlplgyil
      421 alglfftedr ergpralnre qtdewrglmq avvliyhvtg aknvlpiymy lrlinsaylf
      481 lsgyghfcyf wqtgdvslvr farvmfrlnf ltvslclcmn rpyqfyhfvp lvsfwflviy
      541 flawippriy sgslneygpr allylalkll gliwmitily msevffekvf vtrpwkalfv
      601 ttdddireww srwrvdrysv awgvtfgagl valqridhip gtalssvlal isltayttft
      661 ilchsvsece eihsyvafip iigyialrna slalrgkhsa llaglgrisl etlvaqghiw
      721 laadshgvlv llprfpvlnl lvtsfifica sheihrltrv lapyavpndw rlvarnlllf
      781 iavlvpigih dgmf
//
LOCUS       XP_034182262             726 aa            linear   INV 03-SEP-2025
DEFINITION  chromatin licensing and DNA replication factor double parked [Osmia
            lignaria lignaria].
ACCESSION   XP_034182262
VERSION     XP_034182262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..726
                     /product="chromatin licensing and DNA replication factor
                     double parked"
                     /calculated_mol_wt=81752
     Site            order(314..316,318..319,322..325,328,344,374,444..446,449,
                     452,471,480,484..491)
                     /site_type="active"
                     /note="Cdt1-Geminin inhibitory interaction site [active]"
                     /db_xref="CDD:176571"
     Site            order(315..316,318..319,322..325,327,480,484..491,493)
                     /site_type="active"
                     /note="Cdt1-Geminin permissive interaction site [active]"
                     /db_xref="CDD:176571"
     Region          332..506
                     /region_name="CDT1"
                     /note="DNA replication factor CDT1 like; pfam08839"
                     /db_xref="CDD:462618"
     Region          591..716
                     /region_name="Cdt1_c"
                     /note="The C-terminal fold of replication licensing factor
                     Cdt1 is essential for Cdt1 activity and directly interacts
                     with MCM2-7 helicase; cd08767"
                     /db_xref="CDD:176572"
     Site            order(608,612,648..649,653,675,679,683,690,692,695,713,
                     716)
                     /site_type="active"
                     /note="putative Cdt1-MCM complex binding site [active]"
                     /db_xref="CDD:176572"
     CDS             1..726
                     /gene="dup"
                     /coded_by="XM_034326371.2:141..2321"
                     /db_xref="GeneID:117605256"
ORIGIN      
        1 msqplvtsyf ntrkrpatdd lrnkakvlll dredsksvsn qsenqvnkds selsgsltpi
       61 qeeqamgtsp kvilasgkep kgnrsvksns avrniqfdsp ktnaqkalkn nartrvtrsr
      121 qlsgqegqtd iresfqkvte dadakkvlfe kkgalsprkk cpetpkknss siettvtsvf
      181 ndqippnctt pkkssamdkl akqelslnei knrinkssrl melkasiarf knceqklekl
      241 qkqneikkpq iqkfekiqle ipvspqkayk spsktilspm knkellnasp qkrilfepke
      301 ttasvvngsp tkapayqqyl slvesgtpal plpykyrfla eafrcvdtvs ailfnrketi
      361 tfkklkpavq ellrknftle hlaqiktifp dayvynqeky rtfgstskqd kyelvlipvv
      421 eenngrntpd pdnvlktase asmgpqvllh rrrkfynill drvkdehekf llsletpmri
      481 pkekivrwhp efdvescnpi evadlpeppn vekmttakdv ldkakslfnc gtrmekalqr
      541 laeakmtskt lspeknnddv viktengvna vdipvvdtpp atptvqsnni ssalkgipka
      601 llekvrakqa akaleamtrt pnaekeavmy srlpelvkll rnifvaekkg ilplefvttk
      661 lenssraklt iaeleehirl lckllptwts ihhvrkvdyl klqkeidlgk vikrlevman
      721 skvtst
//
LOCUS       XP_034182275             601 aa            linear   INV 03-SEP-2025
DEFINITION  cationic amino acid transporter slimfast [Osmia lignaria lignaria].
ACCESSION   XP_034182275
VERSION     XP_034182275.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326384.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182275.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..601
                     /product="cationic amino acid transporter slimfast"
                     /calculated_mol_wt=65710
     Region          9..576
                     /region_name="2A0303"
                     /note="cationic amino acid transport permease; TIGR00906"
                     /db_xref="CDD:273330"
     CDS             1..601
                     /gene="slif"
                     /coded_by="XM_034326384.2:1..1806"
                     /db_xref="GeneID:117605262"
ORIGIN      
        1 manrlwkals rrrvddsqds kgelarvlgl fdltalgvgs tlgigvyvla gsiaqstagp
       61 avcisfliaa iasafagmcy aefasrvpka gsayvysyvt vgefiafvig wnlileyiig
      121 tasvarglsn yldaligkvi setllsampi rvsflseypd ffafgvvmll villsigvke
      181 ssmlnniftv inlatiliii vagsikadpa nwrirvedid etvrnpgsgg fmpfgitgvm
      241 mgaakcfygf vgfdavattg eeaknpqrni piaivvslvi ilaayfsist vltmmwpyyd
      301 qdpdapfpyv fdkigwptik wivnigavfa lctsllgsmf plprvlyamg sdgvifkrla
      361 nvhsktmtpi fgtvisgllt gtmtlifdlh qliemmsigt llaytivais vlilryqgkg
      421 casntysiaa tnsyklkpid ilkqifnlrn qkevtensyk vakygiimlc ififltalci
      481 nyggtelfss nvvasvilvi liiivllnla avarqptqdi daafkvplvp vlpccsifin
      541 lylmlqldvf twirfavwmf iglsiyffyg ishseqgkrd kieaemmkrk yadqvrivtk
      601 f
//
LOCUS       XP_034182283             547 aa            linear   INV 03-SEP-2025
DEFINITION  doublesex-Mab related 99B [Osmia lignaria lignaria].
ACCESSION   XP_034182283
VERSION     XP_034182283.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326392.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182283.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..547
                     /product="doublesex-Mab related 99B"
                     /calculated_mol_wt=58911
     Region          44..90
                     /region_name="DM"
                     /note="DM DNA binding domain; pfam00751"
                     /db_xref="CDD:459924"
     Region          414..448
                     /region_name="DMA"
                     /note="DMRTA motif; pfam03474"
                     /db_xref="CDD:460937"
     CDS             1..547
                     /gene="dmrt99B"
                     /coded_by="XM_034326392.2:1..1644"
                     /db_xref="GeneID:117605269"
ORIGIN      
        1 mslprgvglg vdvgqlmqhq qqqqqhhvlp aafflrasae ryqrtpkcar crnhgvvsal
       61 kghkrycrwr dcvcakctli aerqrvmaaq valrrqqaqe esearelglq lqyntgsctt
      121 apvlpgaast ttpagsppph pahvtsptgl aipaaaaaaa aaaaahiqtq lqhqqpdcsl
      181 lqrkrlpqde yrqpkiekqd pivggiagtk rsrisesttg tmlstsdtgn eddrdsdsgg
      241 elladrplvp tsagmteneg prkrsnslnd seegspepgs qspthtpplt paltpqredt
      301 papenlslrk sgspqqtqqt lqpvdlvqpr pspplpslaa aatavhfppy aplygpaass
      361 lycspalyqh qhhhhmpepr eiqmqmqmqn iqqtcglqlq qqqqqqqqqq qqqrspvdvl
      421 lrvfpgrrra dveallhrck gdvvsamevl vcedslqpks afsplagala saaaasasvs
      481 ayatraayct tpipstrhrf laapyagtgy lptvikppnq nlhangtgda yreaspddas
      541 dktsyse
//
LOCUS       XP_034182296             400 aa            linear   INV 03-SEP-2025
DEFINITION  general transcription factor IIH subunit 2 Ssl1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182296
VERSION     XP_034182296.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326405.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="general transcription factor IIH subunit 2 Ssl1"
                     /calculated_mol_wt=44852
     Region          4..390
                     /region_name="SSL1"
                     /note="RNA polymerase II transcription
                     initiation/nucleotide excision repair factor TFIIH,
                     subunit SSL1 [Transcription / DNA replication,
                     recombination, and repair]; COG5151"
                     /db_xref="CDD:227480"
     CDS             1..400
                     /gene="Ssl1"
                     /coded_by="XM_034326405.2:175..1377"
                     /db_xref="GeneID:117605277"
ORIGIN      
        1 maddeeekey rwetgyektw eaikeddhgl leasvadiih nakrkrqmnr kigarlgmmr
       61 hlyiildase smsiqdfkpt rflcslklle dfvdeffyqn pisqlgiiit rnkraekvse
      121 lvgnskkhik eiqnmqqitp agepslqnsl elalkslrll pshaskeilv iigalttcdp
      181 gdinetiqsm ktdcvrcsvi glaaelyick rmatatggeh gvalddkhyk eqlnmhidpp
      241 paatrldaal vkmgfphhal hssvndsama vcmchaense esvklistgy lcpqclskhc
      301 elpvecracg ltlvsaphla rsyhylfpve pfdeivydgt rslcygcqkn fsqidkkvyv
      361 cnkcsqtfcl dceifihesl htcpgcatnp dtyqrstdrs
//
LOCUS       XP_034182303             400 aa            linear   INV 03-SEP-2025
DEFINITION  general transcription factor IIH subunit 2 Ssl1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182303
VERSION     XP_034182303.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326412.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="general transcription factor IIH subunit 2 Ssl1"
                     /calculated_mol_wt=44852
     Region          4..390
                     /region_name="SSL1"
                     /note="RNA polymerase II transcription
                     initiation/nucleotide excision repair factor TFIIH,
                     subunit SSL1 [Transcription / DNA replication,
                     recombination, and repair]; COG5151"
                     /db_xref="CDD:227480"
     CDS             1..400
                     /gene="Ssl1"
                     /coded_by="XM_034326412.2:94..1296"
                     /db_xref="GeneID:117605277"
ORIGIN      
        1 maddeeekey rwetgyektw eaikeddhgl leasvadiih nakrkrqmnr kigarlgmmr
       61 hlyiildase smsiqdfkpt rflcslklle dfvdeffyqn pisqlgiiit rnkraekvse
      121 lvgnskkhik eiqnmqqitp agepslqnsl elalkslrll pshaskeilv iigalttcdp
      181 gdinetiqsm ktdcvrcsvi glaaelyick rmatatggeh gvalddkhyk eqlnmhidpp
      241 paatrldaal vkmgfphhal hssvndsama vcmchaense esvklistgy lcpqclskhc
      301 elpvecracg ltlvsaphla rsyhylfpve pfdeivydgt rslcygcqkn fsqidkkvyv
      361 cnkcsqtfcl dceifihesl htcpgcatnp dtyqrstdrs
//
LOCUS       XP_034182312             400 aa            linear   INV 03-SEP-2025
DEFINITION  general transcription factor IIH subunit 2 Ssl1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182312
VERSION     XP_034182312.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326421.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="general transcription factor IIH subunit 2 Ssl1"
                     /calculated_mol_wt=44852
     Region          4..390
                     /region_name="SSL1"
                     /note="RNA polymerase II transcription
                     initiation/nucleotide excision repair factor TFIIH,
                     subunit SSL1 [Transcription / DNA replication,
                     recombination, and repair]; COG5151"
                     /db_xref="CDD:227480"
     CDS             1..400
                     /gene="Ssl1"
                     /coded_by="XM_034326421.2:175..1377"
                     /db_xref="GeneID:117605277"
ORIGIN      
        1 maddeeekey rwetgyektw eaikeddhgl leasvadiih nakrkrqmnr kigarlgmmr
       61 hlyiildase smsiqdfkpt rflcslklle dfvdeffyqn pisqlgiiit rnkraekvse
      121 lvgnskkhik eiqnmqqitp agepslqnsl elalkslrll pshaskeilv iigalttcdp
      181 gdinetiqsm ktdcvrcsvi glaaelyick rmatatggeh gvalddkhyk eqlnmhidpp
      241 paatrldaal vkmgfphhal hssvndsama vcmchaense esvklistgy lcpqclskhc
      301 elpvecracg ltlvsaphla rsyhylfpve pfdeivydgt rslcygcqkn fsqidkkvyv
      361 cnkcsqtfcl dceifihesl htcpgcatnp dtyqrstdrs
//
LOCUS       XP_034182328             400 aa            linear   INV 03-SEP-2025
DEFINITION  general transcription factor IIH subunit 2 Ssl1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182328
VERSION     XP_034182328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="general transcription factor IIH subunit 2 Ssl1"
                     /calculated_mol_wt=44852
     Region          4..390
                     /region_name="SSL1"
                     /note="RNA polymerase II transcription
                     initiation/nucleotide excision repair factor TFIIH,
                     subunit SSL1 [Transcription / DNA replication,
                     recombination, and repair]; COG5151"
                     /db_xref="CDD:227480"
     CDS             1..400
                     /gene="Ssl1"
                     /coded_by="XM_034326437.2:238..1440"
                     /db_xref="GeneID:117605277"
ORIGIN      
        1 maddeeekey rwetgyektw eaikeddhgl leasvadiih nakrkrqmnr kigarlgmmr
       61 hlyiildase smsiqdfkpt rflcslklle dfvdeffyqn pisqlgiiit rnkraekvse
      121 lvgnskkhik eiqnmqqitp agepslqnsl elalkslrll pshaskeilv iigalttcdp
      181 gdinetiqsm ktdcvrcsvi glaaelyick rmatatggeh gvalddkhyk eqlnmhidpp
      241 paatrldaal vkmgfphhal hssvndsama vcmchaense esvklistgy lcpqclskhc
      301 elpvecracg ltlvsaphla rsyhylfpve pfdeivydgt rslcygcqkn fsqidkkvyv
      361 cnkcsqtfcl dceifihesl htcpgcatnp dtyqrstdrs
//
LOCUS       XP_034182318             400 aa            linear   INV 03-SEP-2025
DEFINITION  general transcription factor IIH subunit 2 Ssl1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182318
VERSION     XP_034182318.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326427.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..400
                     /product="general transcription factor IIH subunit 2 Ssl1"
                     /calculated_mol_wt=44852
     Region          4..390
                     /region_name="SSL1"
                     /note="RNA polymerase II transcription
                     initiation/nucleotide excision repair factor TFIIH,
                     subunit SSL1 [Transcription / DNA replication,
                     recombination, and repair]; COG5151"
                     /db_xref="CDD:227480"
     CDS             1..400
                     /gene="Ssl1"
                     /coded_by="XM_034326427.2:175..1377"
                     /db_xref="GeneID:117605277"
ORIGIN      
        1 maddeeekey rwetgyektw eaikeddhgl leasvadiih nakrkrqmnr kigarlgmmr
       61 hlyiildase smsiqdfkpt rflcslklle dfvdeffyqn pisqlgiiit rnkraekvse
      121 lvgnskkhik eiqnmqqitp agepslqnsl elalkslrll pshaskeilv iigalttcdp
      181 gdinetiqsm ktdcvrcsvi glaaelyick rmatatggeh gvalddkhyk eqlnmhidpp
      241 paatrldaal vkmgfphhal hssvndsama vcmchaense esvklistgy lcpqclskhc
      301 elpvecracg ltlvsaphla rsyhylfpve pfdeivydgt rslcygcqkn fsqidkkvyv
      361 cnkcsqtfcl dceifihesl htcpgcatnp dtyqrstdrs
//
LOCUS       XP_034182344             397 aa            linear   INV 03-SEP-2025
DEFINITION  alanine-glyoxylate aminotransferase [Osmia lignaria lignaria].
ACCESSION   XP_034182344
VERSION     XP_034182344.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326453.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="alanine-glyoxylate aminotransferase"
                     /calculated_mol_wt=43738
     Region          28..390
                     /region_name="AGAT_like"
                     /note="Alanine-glyoxylate aminotransferase (AGAT) family.
                     This family belongs to pyridoxal phosphate (PLP)-dependent
                     aspartate aminotransferase superfamily (fold I). The major
                     groups in this CD correspond to alanine-glyoxylate
                     aminotransferase (AGAT); cd06451"
                     /db_xref="CDD:99744"
     Site            order(42,45,52,54,84..85,91,115,118,122..123,212,219,245,
                     267..270,345)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:99744"
     Site            order(85..87,112,162,187,189,212..213)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99744"
     Site            213
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99744"
     CDS             1..397
                     /gene="Agxt"
                     /coded_by="XM_034326453.2:1..1194"
                     /db_xref="GeneID:117605296"
ORIGIN      
        1 mnhewnqlav qkeppkelrt klqlpvktlm gpgptncser vlrslrnqii ghlhpeicqi
       61 mdeikiglqy vfqtknrltl alsasghggm easignllep getilivktg iwgerasdma
      121 nriganveli etqhtmavtl dqletalaky kpvavfmvha esstglkqpl egvgdlvhky
      181 dallivdava slggepffmd awgidvaytg sqkvlgappg ltpisfspqa eeklfrrktk
      241 pcsyyldmli lgvywkcfgn edrvyhhtts atllyglrea laeiaeeglp aswarhaaaa
      301 arlkkglelr glrcyvqipr yqlstvisie lppgvdekii vqramqkykv eisrglgptv
      361 gkilrvgllg inatpkkvdf llraldeglr yarqskl
//
LOCUS       XP_034182358             339 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605302 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182358
VERSION     XP_034182358.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326467.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182358.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="uncharacterized protein LOC117605302 isoform X1"
                     /calculated_mol_wt=38765
     Region          32..59
                     /region_name="zf-LYAR"
                     /note="LYAR-type C2HC zinc finger; pfam08790"
                     /db_xref="CDD:462605"
     CDS             1..339
                     /gene="LOC117605302"
                     /coded_by="XM_034326467.2:237..1256"
                     /db_xref="GeneID:117605302"
ORIGIN      
        1 mvvftcnncg etlqkpkvak hyefhcrrtp fvscvdcfkd frgeeyvaht kcvteaeryg
       61 gkdyvpkpga nkgerkqqew inlvnnmlns eadftsaern flnvlakydn iprkkakfln
      121 fvknimgnrt naaiidsawd kmqtayqndm qkttkqngkv tekvddkkdq dihqennvie
      181 nqnnenicke nqangqsnqs nglhdndnta tsikkkkskk rkvsetmqeq teepiskkka
      241 knnlteetee nkngllsepp vtqcnkgien stfnwkntil divkskeeis lrklqkkvis
      301 qymkfcsdav tqekasskfn kklkkvsevv isdekvrla
//
LOCUS       XP_034182368             339 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605302 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182368
VERSION     XP_034182368.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326477.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182368.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="uncharacterized protein LOC117605302 isoform X1"
                     /calculated_mol_wt=38765
     Region          32..59
                     /region_name="zf-LYAR"
                     /note="LYAR-type C2HC zinc finger; pfam08790"
                     /db_xref="CDD:462605"
     CDS             1..339
                     /gene="LOC117605302"
                     /coded_by="XM_034326477.2:232..1251"
                     /db_xref="GeneID:117605302"
ORIGIN      
        1 mvvftcnncg etlqkpkvak hyefhcrrtp fvscvdcfkd frgeeyvaht kcvteaeryg
       61 gkdyvpkpga nkgerkqqew inlvnnmlns eadftsaern flnvlakydn iprkkakfln
      121 fvknimgnrt naaiidsawd kmqtayqndm qkttkqngkv tekvddkkdq dihqennvie
      181 nqnnenicke nqangqsnqs nglhdndnta tsikkkkskk rkvsetmqeq teepiskkka
      241 knnlteetee nkngllsepp vtqcnkgien stfnwkntil divkskeeis lrklqkkvis
      301 qymkfcsdav tqekasskfn kklkkvsevv isdekvrla
//
LOCUS       XP_034182385             317 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605302 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182385
VERSION     XP_034182385.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326494.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182385.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="uncharacterized protein LOC117605302 isoform X2"
                     /calculated_mol_wt=36192
     Region          <17..37
                     /region_name="zf-LYAR"
                     /note="LYAR-type C2HC zinc finger; pfam08790"
                     /db_xref="CDD:462605"
     CDS             1..317
                     /gene="LOC117605302"
                     /coded_by="XM_034326494.2:222..1175"
                     /db_xref="GeneID:117605302"
ORIGIN      
        1 mrpeyighps yhvlivlkif geeyvahtkc vteaeryggk dyvpkpgank gerkqqewin
       61 lvnnmlnsea dftsaernfl nvlakydnip rkkakflnfv knimgnrtna aiidsawdkm
      121 qtayqndmqk ttkqngkvte kvddkkdqdi hqennvienq nnenickenq angqsnqsng
      181 lhdndntats ikkkkskkrk vsetmqeqte episkkkakn nlteeteenk ngllseppvt
      241 qcnkgienst fnwkntildi vkskeeislr klqkkvisqy mkfcsdavtq ekasskfnkk
      301 lkkvsevvis dekvrla
//
LOCUS       XP_034182395             317 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605302 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182395
VERSION     XP_034182395.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326504.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182395.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="uncharacterized protein LOC117605302 isoform X2"
                     /calculated_mol_wt=36192
     Region          <17..37
                     /region_name="zf-LYAR"
                     /note="LYAR-type C2HC zinc finger; pfam08790"
                     /db_xref="CDD:462605"
     CDS             1..317
                     /gene="LOC117605302"
                     /coded_by="XM_034326504.2:216..1169"
                     /db_xref="GeneID:117605302"
ORIGIN      
        1 mrpeyighps yhvlivlkif geeyvahtkc vteaeryggk dyvpkpgank gerkqqewin
       61 lvnnmlnsea dftsaernfl nvlakydnip rkkakflnfv knimgnrtna aiidsawdkm
      121 qtayqndmqk ttkqngkvte kvddkkdqdi hqennvienq nnenickenq angqsnqsng
      181 lhdndntats ikkkkskkrk vsetmqeqte episkkkakn nlteeteenk ngllseppvt
      241 qcnkgienst fnwkntildi vkskeeislr klqkkvisqy mkfcsdavtq ekasskfnkk
      301 lkkvsevvis dekvrla
//
LOCUS       XP_034182405             336 aa            linear   INV 03-SEP-2025
DEFINITION  putative inositol monophosphatase 3 [Osmia lignaria lignaria].
ACCESSION   XP_034182405
VERSION     XP_034182405.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326514.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="putative inositol monophosphatase 3"
                     /calculated_mol_wt=37204
     Region          70..332
                     /region_name="FIG"
                     /note="FBPase/IMPase/glpX-like domain. A superfamily of
                     metal-dependent phosphatases with various substrates.
                     Fructose-1,6-bisphospatase (both the major and the
                     glpX-encoded variant) hydrolyze fructose-1,6,-bisphosphate
                     to fructose-6-phosphate in...; cl00289"
                     /db_xref="CDD:469707"
     Site            order(90,113..114,154..158,281)
                     /site_type="active"
                     /db_xref="CDD:238814"
     CDS             1..336
                     /gene="LOC117605318"
                     /coded_by="XM_034326514.2:439..1449"
                     /db_xref="GeneID:117605318"
ORIGIN      
        1 mhirmsvrmr rtifcgivll lsvlylytnr ttyssdhekn vslklllaaa ikaaelgase
       61 viavhdqvkf eiqskgktke gindpvtead yrshcamyhs lleafpditv iseeaskdcd
      121 kvtvsnmkds vnslrdynik deivnsndit vwidpldatk eftenllqyv ttmvcvavkg
      181 kpvigviykp feakqnsslf wtwtnhgisk nlqvlpkgee nrppilivsq shagqvhnvt
      241 kiafgkdvqi vsaagagykf levvsknata yvhmtaikkw dicagtailt alggtitqlf
      301 dqqsisfgpn dskiltwgll atmnnhawyl dkfsni
//
LOCUS       XP_034182420             312 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182420
VERSION     XP_034182420.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326529.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="cyclic-AMP response element binding protein B
                     isoform X1"
                     /calculated_mol_wt=33364
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          256..310
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          257..309
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(257,261,263..265,267..269,271..272,274..276)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(275,278,282..283,285..286,289..290,292..293,296,
                     299..300,303..304,306..307)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..312
                     /gene="CrebB"
                     /coded_by="XM_034326529.2:436..1374"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpvsgnv
      241 paytghgvvv edaarkrelr llknreaare crrkkkeyik clenrvaile nrnqtlieel
      301 kslkqlcepk td
//
LOCUS       XP_076546347             312 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546347
VERSION     XP_076546347.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690232.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="cyclic-AMP response element binding protein B
                     isoform X1"
                     /calculated_mol_wt=33364
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          256..310
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          257..309
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(257,261,263..265,267..269,271..272,274..276)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(275,278,282..283,285..286,289..290,292..293,296,
                     299..300,303..304,306..307)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..312
                     /gene="CrebB"
                     /coded_by="XM_076690232.1:484..1422"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpvsgnv
      241 paytghgvvv edaarkrelr llknreaare crrkkkeyik clenrvaile nrnqtlieel
      301 kslkqlcepk td
//
LOCUS       XP_034182430             312 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182430
VERSION     XP_034182430.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326539.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="cyclic-AMP response element binding protein B
                     isoform X1"
                     /calculated_mol_wt=33364
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          256..310
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          257..309
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(257,261,263..265,267..269,271..272,274..276)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(275,278,282..283,285..286,289..290,292..293,296,
                     299..300,303..304,306..307)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..312
                     /gene="CrebB"
                     /coded_by="XM_034326539.2:253..1191"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpvsgnv
      241 paytghgvvv edaarkrelr llknreaare crrkkkeyik clenrvaile nrnqtlieel
      301 kslkqlcepk td
//
LOCUS       XP_034182440             312 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182440
VERSION     XP_034182440.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326549.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="cyclic-AMP response element binding protein B
                     isoform X1"
                     /calculated_mol_wt=33364
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          256..310
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          257..309
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(257,261,263..265,267..269,271..272,274..276)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(275,278,282..283,285..286,289..290,292..293,296,
                     299..300,303..304,306..307)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..312
                     /gene="CrebB"
                     /coded_by="XM_034326549.2:613..1551"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpvsgnv
      241 paytghgvvv edaarkrelr llknreaare crrkkkeyik clenrvaile nrnqtlieel
      301 kslkqlcepk td
//
LOCUS       XP_034182445             312 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182445
VERSION     XP_034182445.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326554.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="cyclic-AMP response element binding protein B
                     isoform X1"
                     /calculated_mol_wt=33364
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          256..310
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          257..309
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(257,261,263..265,267..269,271..272,274..276)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(275,278,282..283,285..286,289..290,292..293,296,
                     299..300,303..304,306..307)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..312
                     /gene="CrebB"
                     /coded_by="XM_034326554.2:492..1430"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpvsgnv
      241 paytghgvvv edaarkrelr llknreaare crrkkkeyik clenrvaile nrnqtlieel
      301 kslkqlcepk td
//
LOCUS       XP_076546348             312 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546348
VERSION     XP_076546348.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690233.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="cyclic-AMP response element binding protein B
                     isoform X1"
                     /calculated_mol_wt=33364
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          256..310
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          257..309
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(257,261,263..265,267..269,271..272,274..276)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(275,278,282..283,285..286,289..290,292..293,296,
                     299..300,303..304,306..307)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..312
                     /gene="CrebB"
                     /coded_by="XM_076690233.1:44..982"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpvsgnv
      241 paytghgvvv edaarkrelr llknreaare crrkkkeyik clenrvaile nrnqtlieel
      301 kslkqlcepk td
//
LOCUS       XP_034182454             306 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182454
VERSION     XP_034182454.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326563.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..306
                     /product="cyclic-AMP response element binding protein B
                     isoform X2"
                     /calculated_mol_wt=32811
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          250..304
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          251..303
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(251,255,257..259,261..263,265..266,268..270)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(269,272,276..277,279..280,283..284,286..287,290,
                     293..294,297..298,300..301)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..306
                     /gene="CrebB"
                     /coded_by="XM_034326563.2:434..1354"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpaytgh
      241 gvvvedaark relrllknre aarecrrkkk eyikclenrv ailenrnqtl ieelkslkql
      301 cepktd
//
LOCUS       XP_034182464             303 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182464
VERSION     XP_034182464.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326573.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="cyclic-AMP response element binding protein B
                     isoform X3"
                     /calculated_mol_wt=32475
     Region          125..162
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          247..301
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          248..300
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(248,252,254..256,258..260,262..263,265..267)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(266,269,273..274,276..277,280..281,283..284,287,
                     290..291,294..295,297..298)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..303
                     /gene="CrebB"
                     /coded_by="XM_034326573.2:435..1346"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitdgr lpplesssec dsnvdsevss
      181 hslhyqtvip agtiqiatqg egvpglhtlt msnaataggt ivqyaqgqdt qffvpghgvv
      241 vedaarkrel rllknreaar ecrrkkkeyi kclenrvail enrnqtliee lkslkqlcep
      301 ktd
//
LOCUS       XP_034182472             282 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182472
VERSION     XP_034182472.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326581.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="cyclic-AMP response element binding protein B
                     isoform X4"
                     /calculated_mol_wt=30106
     Region          125..158
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          226..280
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          227..279
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(227,231,233..235,237..239,241..242,244..246)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(245,248,252..253,255..256,259..260,262..263,266,
                     269..270,273..274,276..277)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..282
                     /gene="CrebB"
                     /coded_by="XM_034326581.2:430..1278"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitvip agtiqiatqg egvpglhtlt
      181 msnaataggt ivqyaqgqdt qffvpvsgnv paytghgvvv edaarkrelr llknreaare
      241 crrkkkeyik clenrvaile nrnqtlieel kslkqlcepk td
//
LOCUS       XP_034182477             279 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182477
VERSION     XP_034182477.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326586.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..279
                     /product="cyclic-AMP response element binding protein B
                     isoform X5"
                     /calculated_mol_wt=29755
     Region          92..129
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          223..277
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          224..276
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(224,228,230..232,234..236,238..239,241..243)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(242,245,249..250,252..253,256..257,259..260,263,
                     266..267,270..271,273..274)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..279
                     /gene="CrebB"
                     /coded_by="XM_034326586.2:443..1282"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitdgrlpp lesssecdsn vdsevsshsl hyqtvipagt iqiatqgegv pglhtltmsn
      181 aataggtivq yaqgqdtqff vpvsgnvpay tghgvvveda arkrelrllk nreaarecrr
      241 kkkeyikcle nrvailenrn qtlieelksl kqlcepktd
//
LOCUS       XP_034182481             279 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182481
VERSION     XP_034182481.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326590.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..279
                     /product="cyclic-AMP response element binding protein B
                     isoform X5"
                     /calculated_mol_wt=29755
     Region          92..129
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          223..277
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          224..276
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(224,228,230..232,234..236,238..239,241..243)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(242,245,249..250,252..253,256..257,259..260,263,
                     266..267,270..271,273..274)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..279
                     /gene="CrebB"
                     /coded_by="XM_034326590.2:487..1326"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitdgrlpp lesssecdsn vdsevsshsl hyqtvipagt iqiatqgegv pglhtltmsn
      181 aataggtivq yaqgqdtqff vpvsgnvpay tghgvvveda arkrelrllk nreaarecrr
      241 kkkeyikcle nrvailenrn qtlieelksl kqlcepktd
//
LOCUS       XP_034182485             279 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182485
VERSION     XP_034182485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..279
                     /product="cyclic-AMP response element binding protein B
                     isoform X5"
                     /calculated_mol_wt=29755
     Region          92..129
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          223..277
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          224..276
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(224,228,230..232,234..236,238..239,241..243)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(242,245,249..250,252..253,256..257,259..260,263,
                     266..267,270..271,273..274)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..279
                     /gene="CrebB"
                     /coded_by="XM_034326594.2:499..1338"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitdgrlpp lesssecdsn vdsevsshsl hyqtvipagt iqiatqgegv pglhtltmsn
      181 aataggtivq yaqgqdtqff vpvsgnvpay tghgvvveda arkrelrllk nreaarecrr
      241 kkkeyikcle nrvailenrn qtlieelksl kqlcepktd
//
LOCUS       XP_076546349             279 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546349
VERSION     XP_076546349.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690234.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..279
                     /product="cyclic-AMP response element binding protein B
                     isoform X5"
                     /calculated_mol_wt=29755
     Region          92..129
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          223..277
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          224..276
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(224,228,230..232,234..236,238..239,241..243)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(242,245,249..250,252..253,256..257,259..260,263,
                     266..267,270..271,273..274)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..279
                     /gene="CrebB"
                     /coded_by="XM_076690234.1:38..877"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitdgrlpp lesssecdsn vdsevsshsl hyqtvipagt iqiatqgegv pglhtltmsn
      181 aataggtivq yaqgqdtqff vpvsgnvpay tghgvvveda arkrelrllk nreaarecrr
      241 kkkeyikcle nrvailenrn qtlieelksl kqlcepktd
//
LOCUS       XP_034182487             276 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182487
VERSION     XP_034182487.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326596.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="cyclic-AMP response element binding protein B
                     isoform X6"
                     /calculated_mol_wt=29552
     Region          125..158
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          220..274
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          221..273
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(221,225,227..229,231..233,235..236,238..240)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(239,242,246..247,249..250,253..254,256..257,260,
                     263..264,267..268,270..271)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..276
                     /gene="CrebB"
                     /coded_by="XM_034326596.2:429..1259"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitvip agtiqiatqg egvpglhtlt
      181 msnaataggt ivqyaqgqdt qffvpaytgh gvvvedaark relrllknre aarecrrkkk
      241 eyikclenrv ailenrnqtl ieelkslkql cepktd
//
LOCUS       XP_034182491             273 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182491
VERSION     XP_034182491.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326600.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="cyclic-AMP response element binding protein B
                     isoform X7"
                     /calculated_mol_wt=29217
     Region          125..158
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          217..271
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          218..270
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(218,222,224..226,228..230,232..233,235..237)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(236,239,243..244,246..247,250..251,253..254,257,
                     260..261,264..265,267..268)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..273
                     /gene="CrebB"
                     /coded_by="XM_034326600.2:430..1251"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqslnrthq qqthhlvast sivqltlpts
       61 attqvqsviq pnqqsviqta tniqpvaisk gnvilvskpn sviqtaqasl qtlqvvetgs
      121 ddsfsdsees peqrggiltr rpsykkilnd lgggeitvip agtiqiatqg egvpglhtlt
      181 msnaataggt ivqyaqgqdt qffvpghgvv vedaarkrel rllknreaar ecrrkkkeyi
      241 kclenrvail enrnqtliee lkslkqlcep ktd
//
LOCUS       XP_034182501             273 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182501
VERSION     XP_034182501.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326610.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="cyclic-AMP response element binding protein B
                     isoform X8"
                     /calculated_mol_wt=29202
     Region          92..129
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          217..271
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          218..270
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(218,222,224..226,228..230,232..233,235..237)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(236,239,243..244,246..247,250..251,253..254,257,
                     260..261,264..265,267..268)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..273
                     /gene="CrebB"
                     /coded_by="XM_034326610.2:440..1261"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitdgrlpp lesssecdsn vdsevsshsl hyqtvipagt iqiatqgegv pglhtltmsn
      181 aataggtivq yaqgqdtqff vpaytghgvv vedaarkrel rllknreaar ecrrkkkeyi
      241 kclenrvail enrnqtliee lkslkqlcep ktd
//
LOCUS       XP_034182510             270 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X9 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182510
VERSION     XP_034182510.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326619.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="cyclic-AMP response element binding protein B
                     isoform X9"
                     /calculated_mol_wt=28866
     Region          92..129
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          214..268
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          215..267
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(215,219,221..223,225..227,229..230,232..234)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(233,236,240..241,243..244,247..248,250..251,254,
                     257..258,261..262,264..265)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..270
                     /gene="CrebB"
                     /coded_by="XM_034326619.2:442..1254"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitdgrlpp lesssecdsn vdsevsshsl hyqtvipagt iqiatqgegv pglhtltmsn
      181 aataggtivq yaqgqdtqff vpghgvvved aarkrelrll knreaarecr rkkkeyikcl
      241 enrvailenr nqtlieelks lkqlcepktd
//
LOCUS       XP_034182519             249 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X10 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182519
VERSION     XP_034182519.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326628.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..249
                     /product="cyclic-AMP response element binding protein B
                     isoform X10"
                     /calculated_mol_wt=26497
     Region          92..125
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          193..247
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          194..246
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(194,198,200..202,204..206,208..209,211..213)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(212,215,219..220,222..223,226..227,229..230,233,
                     236..237,240..241,243..244)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..249
                     /gene="CrebB"
                     /coded_by="XM_034326628.2:436..1185"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitvipagt iqiatqgegv pglhtltmsn aataggtivq yaqgqdtqff vpvsgnvpay
      181 tghgvvveda arkrelrllk nreaarecrr kkkeyikcle nrvailenrn qtlieelksl
      241 kqlcepktd
//
LOCUS       XP_034182527             243 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X11 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182527
VERSION     XP_034182527.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326636.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="cyclic-AMP response element binding protein B
                     isoform X11"
                     /calculated_mol_wt=25943
     Region          92..125
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          187..241
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          188..240
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(188,192,194..196,198..200,202..203,205..207)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(206,209,213..214,216..217,220..221,223..224,227,
                     230..231,234..235,237..238)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..243
                     /gene="CrebB"
                     /coded_by="XM_034326636.2:433..1164"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitvipagt iqiatqgegv pglhtltmsn aataggtivq yaqgqdtqff vpaytghgvv
      181 vedaarkrel rllknreaar ecrrkkkeyi kclenrvail enrnqtliee lkslkqlcep
      241 ktd
//
LOCUS       XP_034182536             240 aa            linear   INV 03-SEP-2025
DEFINITION  cyclic-AMP response element binding protein B isoform X12 [Osmia
            lignaria lignaria].
ACCESSION   XP_034182536
VERSION     XP_034182536.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326645.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="cyclic-AMP response element binding protein B
                     isoform X12"
                     /calculated_mol_wt=25608
     Region          92..125
                     /region_name="pKID"
                     /note="pKID domain; pfam02173"
                     /db_xref="CDD:396650"
     Region          184..238
                     /region_name="bZIP_CREB1"
                     /note="Basic leucine zipper (bZIP) domain of Cyclic
                     AMP-responsive element-binding protein 1 (CREB1) and
                     similar proteins: a DNA-binding and dimerization domain;
                     cd14690"
                     /db_xref="CDD:269838"
     Region          185..237
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269838"
     Site            order(185,189,191..193,195..197,199..200,202..204)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269838"
     Site            order(203,206,210..211,213..214,217..218,220..221,224,
                     227..228,231..232,234..235)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269838"
     CDS             1..240
                     /gene="CrebB"
                     /coded_by="XM_034326645.2:435..1157"
                     /db_xref="GeneID:117605323"
ORIGIN      
        1 mesmveengs avdplstgsq ggdaspaiat svqsviqpnq qsviqtatni qpvaiskgnv
       61 ilvskpnsvi qtaqaslqtl qvvetgsdds fsdseespeq rggiltrrps ykkilndlgg
      121 geitvipagt iqiatqgegv pglhtltmsn aataggtivq yaqgqdtqff vpghgvvved
      181 aarkrelrll knreaarecr rkkkeyikcl enrvailenr nqtlieelks lkqlcepktd
//
LOCUS       XP_034182548             308 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605382 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182548
VERSION     XP_034182548.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326657.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="uncharacterized protein LOC117605382 isoform X1"
                     /calculated_mol_wt=35772
     Region          11..100
                     /region_name="Myb_DNA-bind_4"
                     /note="Myb/SANT-like DNA-binding domain; pfam13837"
                     /db_xref="CDD:463994"
     CDS             1..308
                     /gene="LOC117605382"
                     /coded_by="XM_034326657.2:123..1049"
                     /db_xref="GeneID:117605382"
ORIGIN      
        1 mtqsaqytkf rwtenltcrf iqlrkengkl ftgrkysaqa gweyilqqmr qefpsvmanv
       61 hyrvlkkkws nllqqykelr npvhgdrnka ddvswpfysa idqvlghdra tspindeyid
      121 pheflcvsvt peeptssvdt lpagsnpeiv rragdssaep rlqtfgddcs ieikripsne
      181 ptrqtgkyyg rrkrdsknsm prlpratglt ikkvtseadc sskdssrvrg nelvdsigdk
      241 eravtarned eerprksrrs ripyredhes rtdrrieqif eyikrrdeen rsimirvmha
      301 vetiankl
//
LOCUS       XP_034182556             303 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605382 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182556
VERSION     XP_034182556.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326665.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="uncharacterized protein LOC117605382 isoform X2"
                     /calculated_mol_wt=34929
     CDS             1..303
                     /gene="LOC117605382"
                     /coded_by="XM_034326665.2:16..927"
                     /db_xref="GeneID:117605382"
ORIGIN      
        1 mlplcagten ltcrfiqlrk engklftgrk ysaqagweyi lqqmrqefps vmanvhyrvl
       61 kkkwsnllqq ykelrnpvhg drnkaddvsw pfysaidqvl ghdratspin deyidphefl
      121 cvsvtpeept ssvdtlpags npeivrragd ssaeprlqtf gddcsieikr ipsneptrqt
      181 gkyygrrkrd sknsmprlpr atgltikkvt seadcsskds srvrgnelvd sigdkeravt
      241 arnedeerpr ksrrsripyr edhesrtdrr ieqifeyikr rdeenrsimi rvmhavetia
      301 nkl
//
LOCUS       XP_034182563             297 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605382 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182563
VERSION     XP_034182563.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326672.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..297
                     /product="uncharacterized protein LOC117605382 isoform X3"
                     /calculated_mol_wt=34079
     CDS             1..297
                     /gene="LOC117605382"
                     /coded_by="XM_034326672.2:154..1047"
                     /db_xref="GeneID:117605382"
ORIGIN      
        1 mksfhtavlv hvtpvsndpp hdavctvhei siyilqqmrq efpsvmanvh yrvlkkkwsn
       61 llqqykelrn pvhgdrnkad dvswpfysai dqvlghdrat spindeyidp heflcvsvtp
      121 eeptssvdtl pagsnpeivr ragdssaepr lqtfgddcsi eikripsnep trqtgkyygr
      181 rkrdsknsmp rlpratglti kkvtseadcs skdssrvrgn elvdsigdke ravtarnede
      241 erprksrrsr ipyredhesr tdrrieqife yikrrdeenr simirvmhav etiankl
//
LOCUS       XP_034182576             222 aa            linear   INV 03-SEP-2025
DEFINITION  p53 apoptosis effector related to PMP-22 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182576
VERSION     XP_034182576.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326685.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="p53 apoptosis effector related to PMP-22 isoform
                     X1"
                     /calculated_mol_wt=24046
     Region          26..181
                     /region_name="PMP22_Claudin"
                     /note="PMP-22/EMP/MP20/Claudin family; cl21598"
                     /db_xref="CDD:473919"
     CDS             1..222
                     /gene="LOC117605396"
                     /coded_by="XM_034326685.2:630..1298"
                     /db_xref="GeneID:117605396"
ORIGIN      
        1 mimytvaedh mgdteiaqvi aficgiivil lmimglastd wlmalgwrqg lfahcieega
       61 ptpvpfnlpd tagcyqardv aymkaaaalc vvclltdiaa tiltglglrs qdnrekhryy
      121 rfavfcmgfa lvsllialvv ypvcfateln lgnrtswefg waygvgwgaa iflfggavll
      181 lcdkeseeiy ykerkivrdd iggggsmigm ghhggrsgtq la
//
LOCUS       XP_034182585             221 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 47 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182585
VERSION     XP_034182585.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326694.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="transmembrane protein 47 isoform X2"
                     /calculated_mol_wt=23935
     Region          25..180
                     /region_name="PMP22_Claudin"
                     /note="PMP-22/EMP/MP20/Claudin family; cl21598"
                     /db_xref="CDD:473919"
     CDS             1..221
                     /gene="LOC117605396"
                     /coded_by="XM_034326694.2:200..865"
                     /db_xref="GeneID:117605396"
ORIGIN      
        1 mpqtttieti titrplkvia ficgiivill mimglastdw lmalgwrqgl fahcieegap
       61 tpvpfnlpdt agcyqardva ymkaaaalcv vclltdiaat iltglglrsq dnrekhryyr
      121 favfcmgfal vsllialvvy pvcfatelnl gnrtswefgw aygvgwgaai flfggavlll
      181 cdkeseeiyy kerkivrddi ggggsmigmg hhggrsgtql a
//
LOCUS       XP_034182591             221 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 47 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182591
VERSION     XP_034182591.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326700.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="transmembrane protein 47 isoform X2"
                     /calculated_mol_wt=23935
     Region          25..180
                     /region_name="PMP22_Claudin"
                     /note="PMP-22/EMP/MP20/Claudin family; cl21598"
                     /db_xref="CDD:473919"
     CDS             1..221
                     /gene="LOC117605396"
                     /coded_by="XM_034326700.2:158..823"
                     /db_xref="GeneID:117605396"
ORIGIN      
        1 mpqtttieti titrplkvia ficgiivill mimglastdw lmalgwrqgl fahcieegap
       61 tpvpfnlpdt agcyqardva ymkaaaalcv vclltdiaat iltglglrsq dnrekhryyr
      121 favfcmgfal vsllialvvy pvcfatelnl gnrtswefgw aygvgwgaai flfggavlll
      181 cdkeseeiyy kerkivrddi ggggsmigmg hhggrsgtql a
//
LOCUS       XP_034182599             221 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 47 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182599
VERSION     XP_034182599.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326708.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="transmembrane protein 47 isoform X2"
                     /calculated_mol_wt=23935
     Region          25..180
                     /region_name="PMP22_Claudin"
                     /note="PMP-22/EMP/MP20/Claudin family; cl21598"
                     /db_xref="CDD:473919"
     CDS             1..221
                     /gene="LOC117605396"
                     /coded_by="XM_034326708.2:243..908"
                     /db_xref="GeneID:117605396"
ORIGIN      
        1 mpqtttieti titrplkvia ficgiivill mimglastdw lmalgwrqgl fahcieegap
       61 tpvpfnlpdt agcyqardva ymkaaaalcv vclltdiaat iltglglrsq dnrekhryyr
      121 favfcmgfal vsllialvvy pvcfatelnl gnrtswefgw aygvgwgaai flfggavlll
      181 cdkeseeiyy kerkivrddi ggggsmigmg hhggrsgtql a
//
LOCUS       XP_034182609             221 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 47 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182609
VERSION     XP_034182609.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326718.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="transmembrane protein 47 isoform X2"
                     /calculated_mol_wt=23935
     Region          25..180
                     /region_name="PMP22_Claudin"
                     /note="PMP-22/EMP/MP20/Claudin family; cl21598"
                     /db_xref="CDD:473919"
     CDS             1..221
                     /gene="LOC117605396"
                     /coded_by="XM_034326718.2:132..797"
                     /db_xref="GeneID:117605396"
ORIGIN      
        1 mpqtttieti titrplkvia ficgiivill mimglastdw lmalgwrqgl fahcieegap
       61 tpvpfnlpdt agcyqardva ymkaaaalcv vclltdiaat iltglglrsq dnrekhryyr
      121 favfcmgfal vsllialvvy pvcfatelnl gnrtswefgw aygvgwgaai flfggavlll
      181 cdkeseeiyy kerkivrddi ggggsmigmg hhggrsgtql a
//
LOCUS       XP_034182621             198 aa            linear   INV 03-SEP-2025
DEFINITION  thioredoxin domain-containing protein 12 [Osmia lignaria lignaria].
ACCESSION   XP_034182621
VERSION     XP_034182621.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326730.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..198
                     /product="thioredoxin domain-containing protein 12"
                     /calculated_mol_wt=22944
     Region          43..158
                     /region_name="Protein Disulfide Oxidoreductases and Other
                     Proteins with a Thioredoxin fold"
                     /note="The thioredoxin (TRX)-like superfamily is a large,
                     diverse group of proteins containing a TRX fold. Many
                     members contain a classic TRX domain with a redox active
                     CXXC motif. They function as protein disulfide
                     oxidoreductases (PDOs), altering the redox...; cl00388"
                     /db_xref="CDD:469754"
     CDS             1..198
                     /gene="LOC117605415"
                     /coded_by="XM_034326730.2:47..643"
                     /db_xref="GeneID:117605415"
ORIGIN      
        1 miqcikylss lnfinlandi vanniltvkd tnsdagfdql fkwksfsngf neaktlkkpv
       61 flmihkefcp acqrlkekfa kcvrlmdisd rfvmvkvefe gqpfltekkf qpdgkyvpri
      121 lfytadggfi eeaynkhrda dkehkyfyss ptqiidtmmy vlkkyskeps llmfqndhps
      181 kpevcdvdnd iivptilh
//
LOCUS       XP_034182636             198 aa            linear   INV 03-SEP-2025
DEFINITION  mpv17-like protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034182636
VERSION     XP_034182636.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182636.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..198
                     /product="mpv17-like protein 2"
                     /calculated_mol_wt=22957
     Region          121..182
                     /region_name="Mpv17_PMP22"
                     /note="Mpv17 / PMP22 family; pfam04117"
                     /db_xref="CDD:461182"
     CDS             1..198
                     /gene="LOC117605421"
                     /coded_by="XM_034326745.2:363..959"
                     /db_xref="GeneID:117605421"
ORIGIN      
        1 mnitrnissv inristvkel lfsskyllyt nvaisislsa tgdvleqhye ilknewdkws
       61 snrtknmais gmsigivchy wyryldarlp grtfdivlkk vvidqlvcsp lcismffltl
      121 gilensswae lkaeiinkay klyvaewivw ppaqifnfcf lptryrvlyd ntislgydvy
      181 tshvkhdnlm ynsknyih
//
LOCUS       XP_034182652             182 aa            linear   INV 03-SEP-2025
DEFINITION  ADP-ribosylation factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034182652
VERSION     XP_034182652.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326761.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..182
                     /product="ADP-ribosylation factor 1"
                     /calculated_mol_wt=20549
     Region          5..179
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            24..31
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206717"
     Site            order(26..32,126..127,129,159..161)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206717"
     Site            order(26..27,31,35,47..54,76,81)
                     /site_type="other"
                     /note="putative GAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206717"
     Site            36..51
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206717"
     Site            order(48..52,57,67,71,73,77,79..81)
                     /site_type="other"
                     /note="GEF interaction site [polypeptide binding]"
                     /db_xref="CDD:206717"
     Site            48
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206717"
     Site            order(49..51,73,80)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206717"
     Site            51..66
                     /site_type="active"
                     /note="interswitch region [active]"
                     /db_xref="CDD:206717"
     Site            67..70
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206717"
     Site            69..86
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206717"
     Site            126..129
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206717"
     Site            159..161
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206717"
     CDS             1..182
                     /gene="Arf79F"
                     /coded_by="XM_034326761.2:387..935"
                     /db_xref="GeneID:117605422"
ORIGIN      
        1 mgnmfatlfk glfgkkemri lmvgldaagk ttilyklklg eivttiptig fnvetveykn
       61 isftvwdvgg qdkirplwrh yfqntqglif vvdsndreri geareelmrm laedelrdav
      121 llifankqdl pnamnaaeit dklglhslrn rnwyiqatca tsgdglyegl dwlsnqlksa
      181 nr
//
LOCUS       XP_034182661             182 aa            linear   INV 03-SEP-2025
DEFINITION  ADP-ribosylation factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034182661
VERSION     XP_034182661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..182
                     /product="ADP-ribosylation factor 1"
                     /calculated_mol_wt=20549
     Region          5..179
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            24..31
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206717"
     Site            order(26..32,126..127,129,159..161)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206717"
     Site            order(26..27,31,35,47..54,76,81)
                     /site_type="other"
                     /note="putative GAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206717"
     Site            36..51
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206717"
     Site            order(48..52,57,67,71,73,77,79..81)
                     /site_type="other"
                     /note="GEF interaction site [polypeptide binding]"
                     /db_xref="CDD:206717"
     Site            48
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206717"
     Site            order(49..51,73,80)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206717"
     Site            51..66
                     /site_type="active"
                     /note="interswitch region [active]"
                     /db_xref="CDD:206717"
     Site            67..70
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206717"
     Site            69..86
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206717"
     Site            126..129
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206717"
     Site            159..161
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206717"
     CDS             1..182
                     /gene="Arf79F"
                     /coded_by="XM_034326770.2:156..704"
                     /db_xref="GeneID:117605422"
ORIGIN      
        1 mgnmfatlfk glfgkkemri lmvgldaagk ttilyklklg eivttiptig fnvetveykn
       61 isftvwdvgg qdkirplwrh yfqntqglif vvdsndreri geareelmrm laedelrdav
      121 llifankqdl pnamnaaeit dklglhslrn rnwyiqatca tsgdglyegl dwlsnqlksa
      181 nr
//
LOCUS       XP_034182670             161 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-conjugating enzyme E2 W [Osmia lignaria lignaria].
ACCESSION   XP_034182670
VERSION     XP_034182670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326779.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="ubiquitin-conjugating enzyme E2 W"
                     /calculated_mol_wt=18213
     Region          15..135
                     /region_name="UBCc_UBE2W"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin conjugating enzyme E2 W and related
                     enzymes; cd23808"
                     /db_xref="CDD:467428"
     CDS             1..161
                     /gene="LOC117605430"
                     /coded_by="XM_034326779.2:63..548"
                     /db_xref="GeneID:117605430"
ORIGIN      
        1 mesdittmaa mspskrrlqk elmslirepp pgvhvdedla gqnltqwivh megakgtlye
       61 gerfqlqfkf sskypfdspe vtfiggnipv hphvysnghi clsiltddws palsvqsvcl
      121 sivsmlssck ekkrppdnlf yvrtcnknpk ktkwwyhdds v
//
LOCUS       XP_034182697              64 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein L33 [Osmia lignaria lignaria].
ACCESSION   XP_034182697
VERSION     XP_034182697.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326806.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..64
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..64
                     /product="mitochondrial ribosomal protein L33"
                     /calculated_mol_wt=7349
     CDS             1..64
                     /gene="mRpL33"
                     /coded_by="XM_034326806.2:143..337"
                     /db_xref="GeneID:117605445"
ORIGIN      
        1 mfltnillkk akskrvlvsi ksvatghmrn qirertadki etvffdpwvq amsvykevrk
       61 iksi
//
LOCUS       XP_076546353             240 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305692 [Osmia lignaria lignaria].
ACCESSION   XP_076546353
VERSION     XP_076546353.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690238.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 23% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="uncharacterized protein LOC143305692"
                     /calculated_mol_wt=26608
     Region          161..181
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..240
                     /gene="LOC143305692"
                     /coded_by="XM_076690238.1:1..723"
                     /db_xref="GeneID:143305692"
ORIGIN      
        1 mtmekkaied givacdrfkt taifsrkktt leqdgrlkdl dltrkiient raivlrtnra
       61 lsrryqlliv pskplfqlls gffaslgwnl dgtrrgsrqs andvpapltp qrrarlhcps
      121 rhsgrnnnnn nnnnnnnnnn nnnssrsssv svsgldrrhn csrcgksykn ayilkrhlly
      181 ecgkapsfnc phcafsskye rnlkahinhr hvapqssasa svtastasat attaavnqra
//
LOCUS       XP_034180866             175 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604650 [Osmia lignaria lignaria].
ACCESSION   XP_034180866
VERSION     XP_034180866.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034324975.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="uncharacterized protein LOC117604650"
                     /calculated_mol_wt=18594
     Region          67..114
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..175
                     /gene="LOC117604650"
                     /coded_by="XM_034324975.2:1..528"
                     /db_xref="GeneID:117604650"
ORIGIN      
        1 mtspetvfrs lilwlvvlgs wwccagekls pnqkapqfar gsgglildtp yddnkarktp
       61 vssgnvvqdg vqrdetemlk lvdgelvrtv kgsfsynspe glpisvkyea dengnrasfk
      121 fgtgetgaig agpstrpgqk qngnrggqtq qgskgskgss ylppndanrs ylppk
//
LOCUS       XP_076546355             729 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604663 [Osmia lignaria lignaria].
ACCESSION   XP_076546355
VERSION     XP_076546355.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690240.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="uncharacterized protein LOC117604663"
                     /calculated_mol_wt=77667
     Region          <106..>255
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          367..613
                     /region_name="Zona_pellucida"
                     /note="Zona pellucida-like domain; pfam00100"
                     /db_xref="CDD:459673"
     CDS             1..729
                     /gene="LOC117604663"
                     /coded_by="XM_076690240.1:115..2304"
                     /db_xref="GeneID:117604663"
ORIGIN      
        1 mkqivgtlli vaglgvatsq eyggrhalfv rapsvaahsr vqraapvsaa dlmaqnildw
       61 iqgiyrqgar ktrqqsdpag ylppystqrp perprpfqpp stgqqpsyss psaqnevtnn
      121 ypyqrpsgqs stvqpplpfs tqrstylppn spssynprps ptpsysnsgs safppyssts
      181 rpvsttqssq pgysssggvs snfgyssstf agagsgtntg kfstpsgfss tygystqrpn
      241 tayipsaegg assgtygyst snpsppfpts gtgtgtgtgv gtgtgtgvga gtgtyeqdsg
      301 agypapsyps tpqenaagsn gpsetpggtg istgqgsgss gtvigdgage dddlkhpphi
      361 haldvqcskt mmtidiefnr afdgviyskg yytnpecryv aqnsgqtkys ftvsldscgt
      421 qfindfagea gqaylenvlv lqnepgiqev wdtvrrvrcl wegninkals vnfsvdmlnq
      481 eivtfsgdta varldiqvgk gpfaaaadgl vkigetmtlv vsvegdpafd lqvrdclard
      541 esstnmiqlt dergcilkpk lfgafqktkd tgntgasiia yayfqafkfp dvmdlfiecn
      601 velcktdcep cpelnqqiep grrrrrsily ttassntsav rlsepirvtr gfrvvmvddl
      661 skassqvleh leetaieema rtttnvcmty tgfytaiffl lstifvatis avtlyvklqr
      721 vyrsksiys
//
LOCUS       XP_076546356             128 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117604956 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546356
VERSION     XP_076546356.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690241.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 25% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..128
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117604956"
                     /calculated_mol_wt=14642
     CDS             1..128
                     /gene="LOC117604956"
                     /coded_by="XM_076690241.1:1..387"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon"
                     /db_xref="GeneID:117604956"
ORIGIN      
        1 mrrdtmmstt mieglggvht gtmycfvpvp ftdcvsvyka cqdptfyedn leaemeklra
       61 hivlfvllvs falvysmpyp ggghhehthf vihvpelihk hhhthvkkih ikshdeegdd
      121 ghehieew
//
LOCUS       XP_034180906             245 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604666 [Osmia lignaria lignaria].
ACCESSION   XP_034180906
VERSION     XP_034180906.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325015.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180906.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..245
                     /product="uncharacterized protein LOC117604666"
                     /calculated_mol_wt=29210
     CDS             1..245
                     /gene="LOC117604666"
                     /coded_by="XM_034325015.2:1..738"
                     /db_xref="GeneID:117604666"
ORIGIN      
        1 mysrnwlfky lfllvlnkiv vneavhlkiy vpdvvnhhih tktvllhvhk papkkpkhhk
       61 kheyhanwss wksygndhtf kdhqefpeel eepkhevvtk kpkdqkdrqk ylpvygyhkd
      121 syfpqsymed ndleekgrqr gkgpsyavhe evndippnte sfaynyeegy rkglqeeagh
      181 iradqmskfh edhqeeqdds enegsrdeye gktdagryfv ddveyentrd krdgrvgrfr
      241 riiki
//
LOCUS       XP_034180920              66 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604674 [Osmia lignaria lignaria].
ACCESSION   XP_034180920
VERSION     XP_034180920.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325029.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..66
                     /product="uncharacterized protein LOC117604674"
                     /calculated_mol_wt=7706
     CDS             1..66
                     /gene="LOC117604674"
                     /coded_by="XM_034325029.1:1..201"
                     /db_xref="GeneID:117604674"
ORIGIN      
        1 mnrkflyaaa llafllllqq pteskkvivh vpyrvknlkh thtiyvviph ydekeedske
       61 dekled
//
LOCUS       XP_034192578             524 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609904 [Osmia lignaria lignaria].
ACCESSION   XP_034192578
VERSION     XP_034192578.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336687.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192578.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..524
                     /product="uncharacterized protein LOC117609904"
                     /calculated_mol_wt=59554
     CDS             1..524
                     /gene="LOC117609904"
                     /coded_by="XM_034336687.2:511..2085"
                     /db_xref="GeneID:117609904"
ORIGIN      
        1 mkiarvvtvc aalistslga eterskrraq pksqlpqpri enisttpvtf ttlrpdirpf
       61 gsqidpsvyp vgfrrrapri rttrfasirs rnnrhrrqlh rrgkksrlgr sfdvtnskkl
      121 elvhgppdei hlepiyrvik nknifdsiid lirrligskk plgplvgpfh fpgvgdkvyv
      181 rllepvlpnh lvirlvshlp ateieaamek hisppihipk lsqipsishe sfvvapneli
      241 dsfskhslaa sdsllsssnh avqtsktakk ldekmlgnsw nnrvhisety tkndhrnstn
      301 pqplwspeis rddwkfidls nessyqdsfn qvyesskepe vlkfsiedfh dplnvtdyqp
      361 svnsfipvrd yalnnefkna evspkysldf vskkmrylnl dgsvdsseek ndgslksven
      421 spifskvdre wglvrrmrvr rkeiktssns ekgvqernst eafpevdrks dddrsnysmn
      481 klrnntvtgl kedgnfgqry knnstsvntt ksesiknste srgi
//
LOCUS       XP_076546357             163 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117604682 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546357
VERSION     XP_076546357.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690242.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 27% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..163
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117604682"
                     /calculated_mol_wt=18933
     CDS             1..163
                     /gene="LOC117604682"
                     /coded_by="XM_076690242.1:139..630"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:117604682"
ORIGIN      
        1 millthvted derrwpmqma rtrdsfvhgr rnmvveergt vehtstgtlc kfahevrvvp
       61 rgpphrprrr rlsggireri rrqmnkppdd fmdasparsq qsfafpkacn esrganerrk
      121 nsrscntvvg rryglptgay rytqalcrte veyglywrhl lhg
//
LOCUS       XP_034180941            1630 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604688 [Osmia lignaria lignaria].
ACCESSION   XP_034180941
VERSION     XP_034180941.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325050.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034180941.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1630
                     /product="uncharacterized protein LOC117604688"
                     /calculated_mol_wt=182242
     Region          52..529
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(200,203..208,210..211)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(203,315..316,338..343,421,433,436,447,528)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(203,241..242,288,290..291,294,315..316,338..343,421,
                     433,436,444..447,509)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(241,290..291,294,315,444..446,502,509)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Region          594..1072
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(743,746..751,753..754)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(746,858..859,881..886,964,976,979,990,1071)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(746,784..785,831,833..834,837,858..859,881..886,964,
                     976,979,987..990,1052)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(784,833..834,837,858,987..989,1045,1052)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Region          1140..1616
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(1288,1291..1296,1298..1299)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(1291,1401..1402,1424..1429,1507,1519,1522,1533,1614)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(1291,1327..1328,1374,1376..1377,1380,1401..1402,
                     1424..1429,1507,1519,1522,1530..1533,1595)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(1327,1376..1377,1380,1401,1530..1532,1588,1595)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..1630
                     /gene="LOC117604688"
                     /coded_by="XM_034325050.2:144..5036"
                     /db_xref="GeneID:117604688"
ORIGIN      
        1 mvadseinnv elpfrienni lkgkvipfks qytsvgkllf dflknnlnfv gqvdaltdie
       61 dtfeeildrs ikcalwlqkq gvgsndiivi sshnhldcfi pciaalfiga ifnpwhsdvn
      121 tqlarhfmsl sqpkvvfane ksapvvleaa kiechhlkvv tfgeylgtss fsdilkghsk
      181 ssvanfqcae iddpeqtavi lfssgttgpp kgvqlphraf lkfleldvef slknhvsvwf
      241 stlywlsgtl lnlksivscs kkiivansde etackiveky kvswmmlsts mvnrlarynn
      301 lqhydlsslr nlytggssmg keaedlarkr fpqtlllqay gmtelgnlla tqvegstsgs
      361 cgipatncei kiidpktgkv lgpnqngeic akivsmmtgy yknpeatknt idedgwlhsg
      421 dlgyynekge lfiidrlkel ikfqgfqiap teiegvlqsh pavlevavvs mphpldcehp
      481 vafiskvptk evtaaelmml vannlmdycq lrggvkflps lphtpsgkia rnelkkmaka
      541 maldmanink qyvekpfrie nnimkgkvmp ykppyksigk llfdhmkkhp nfvaqvdatt
      601 giedtlkeml drsircalwl qkqgvkkndi vvisshnhrd dficcmaalf igasynswyh
      661 einlqlarhf trltkpkivf aneisvplvi eaakleghyp kivtfgeypg ttpyseilng
      721 hsessvanfq cteiddpeqt aaivfssgtt gmpkgvqlph rsilgyvele dnfplgnqiv
      781 mwfstlywls gtlmcsksiv qlsrrvvtpt fdeeiackii ekykvswlgl tasmmnclar
      841 ynrlqdfdls slklfytgga fvgknsedll rkrfphtrvv qiygmtelgm vvteqtedse
      901 sgscgkaikn ceikivdvet gkvlgpfqng elwvkspwim tgyykdpeat kniideegwv
      961 hsgdigyyne kgdifivdri kdmikcqahq vvpsqieevi qrhsavleva vvsmphpidt
     1021 eqpiafvski pnkevteeel iklvadnlvd ysqlrggvkf lpalphthsg kiskkelkel
     1081 aktmapcfnm svnnkndgqp rftiennilk grvvpfqsqy thigqllfns mkknanlvgq
     1141 vdaitgiedt fgdildmsik calwmqkqgv ksndiiviss qsncevfipc iaalfigaif
     1201 npwddekdtk ttryyiklmq pkaifageks apialeaakl edhptkvvcf gdypgttrfa
     1261 dilkghsess vinfqctdie dprqtaiimf ssgttslpkg iplthqsllk llevkeepsv
     1321 dtwmwysplf wisgpslnmk niifhrkkiv pppfeeesac kiiekykvel vllnttrinr
     1381 lirhnrfyey dlsslkvlyi igspmekvvq dlvkkcfpht viaqvyglte tgyiitaqsa
     1441 nspsgssgli nhnceikiid letgktlgpn qkgeicgksi smmsgyyknp eatkkvided
     1501 gwlhtsdlgy yteegelfvv drlsdlikfr dhliipseie avllthpsvl evavigkphp
     1561 idgehplafv skvpnkkvsa eelidlvask lmdhchlrgg vifllsfpyt ptgkilkkel
     1621 kmmlkevtih
//
LOCUS       XP_034179495             652 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoinositide-dependent kinase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179495
VERSION     XP_034179495.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323604.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179495.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..652
                     /product="phosphoinositide-dependent kinase 1 isoform X1"
                     /calculated_mol_wt=73395
     Region          135..420
                     /region_name="STKc_PDK1"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Phosphoinositide-dependent kinase 1; cd05581"
                     /db_xref="CDD:270733"
     Site            order(143..147,151,164,166,200,216..217,219,223,225,262,
                     264,266..267,269,279..280,283,319..324,350,356,359)
                     /site_type="active"
                     /db_xref="CDD:270733"
     Site            order(143..146,149,151,164,166,185,200,216..219,222..223,
                     266..267,269,279..280)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270733"
     Site            order(147,223,225,262,264,266,283,319..324,350,356,359)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270733"
     Site            order(279..288,294,316..324)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270733"
     Region          537..643
                     /region_name="PH_PDK1"
                     /note="3-Phosphoinositide dependent protein kinase 1
                     (PDK1) pleckstrin homology (PH) domain; cd01262"
                     /db_xref="CDD:241293"
     Site            order(561,563,568,570,582,591,617)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:241293"
     CDS             1..652
                     /gene="Pdk1"
                     /coded_by="XM_034323604.2:31..1989"
                     /db_xref="GeneID:117603960"
ORIGIN      
        1 mlagfkylrg aaplldsygl twkttgpddl vptpqpmpks eecgtpedsa vkdtetesds
       61 nvacnpatrv anqaltvspq pavtvegpvs kmspptndvt ngvvappntl aprvsptanp
      121 alttinpptp krnpkdfifg kvigegsfst vylakdihsg keyaikvcdk rhiikekkte
      181 yvkrekevln mladakhsfv rlfctfqdve rlyfvlsyak ngellpyink vgsfdiectk
      241 fysaeilrgl eylhglgiih rdlkpenill dekmhvlitd fgsakilkdp etvsthttat
      301 ddpqqqqqqq yrrerrgsfv gtaqyvspel ltdktsrasd lwalgcivyq mvaglppfrs
      361 rseymifqki lkleyeipdg fcelarslvs qllvlepser lgaqdehgag ypsirahpff
      421 egvdfetlhe qtpppiypyl pgtseheelr sqyrvpdhle pglddkqltr llglgigedd
      481 ddddddddde datierekva qqatkvvekp rrrtnavatd gnitdltpee iknrlekqra
      541 snqwdvfveg nlilkqgfvn krkglfarrr mlllttgphl yyvdpvnmvl kgeipwspel
      601 rvepksfkif fvhtpnrtyy ledpegfale wcraientrv hyyglqestt ta
//
LOCUS       XP_034179502             631 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoinositide-dependent kinase 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179502
VERSION     XP_034179502.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179502.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="phosphoinositide-dependent kinase 1 isoform X2"
                     /calculated_mol_wt=71123
     Region          114..399
                     /region_name="STKc_PDK1"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Phosphoinositide-dependent kinase 1; cd05581"
                     /db_xref="CDD:270733"
     Site            order(122..126,130,143,145,179,195..196,198,202,204,241,
                     243,245..246,248,258..259,262,298..303,329,335,338)
                     /site_type="active"
                     /db_xref="CDD:270733"
     Site            order(122..125,128,130,143,145,164,179,195..198,201..202,
                     245..246,248,258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270733"
     Site            order(126,202,204,241,243,245,262,298..303,329,335,338)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270733"
     Site            order(258..267,273,295..303)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270733"
     Region          516..622
                     /region_name="PH_PDK1"
                     /note="3-Phosphoinositide dependent protein kinase 1
                     (PDK1) pleckstrin homology (PH) domain; cd01262"
                     /db_xref="CDD:241293"
     Site            order(540,542,547,549,561,570,596)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:241293"
     CDS             1..631
                     /gene="Pdk1"
                     /coded_by="XM_034323611.2:101..1996"
                     /db_xref="GeneID:117603960"
ORIGIN      
        1 mtdspaalie ddpsqkekse ecgtpedsav kdtetesdsn vacnpatrva nqaltvspqp
       61 avtvegpvsk mspptndvtn gvvappntla prvsptanpa lttinpptpk rnpkdfifgk
      121 vigegsfstv ylakdihsgk eyaikvcdkr hiikekktey vkrekevlnm ladakhsfvr
      181 lfctfqdver lyfvlsyakn gellpyinkv gsfdiectkf ysaeilrgle ylhglgiihr
      241 dlkpenilld ekmhvlitdf gsakilkdpe tvsthttatd dpqqqqqqqy rrerrgsfvg
      301 taqyvspell tdktsrasdl walgcivyqm vaglppfrsr seymifqkil kleyeipdgf
      361 celarslvsq llvlepserl gaqdehgagy psirahpffe gvdfetlheq tpppiypylp
      421 gtseheelrs qyrvpdhlep glddkqltrl lglgigeddd dddddddded atierekvaq
      481 qatkvvekpr rrtnavatdg nitdltpeei knrlekqras nqwdvfvegn lilkqgfvnk
      541 rkglfarrrm lllttgphly yvdpvnmvlk geipwspelr vepksfkiff vhtpnrtyyl
      601 edpegfalew craientrvh yyglqesttt a
//
LOCUS       XP_034179511             596 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoinositide-dependent kinase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179511
VERSION     XP_034179511.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323620.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179511.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="phosphoinositide-dependent kinase 1 isoform X3"
                     /calculated_mol_wt=67606
     Region          79..364
                     /region_name="STKc_PDK1"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Phosphoinositide-dependent kinase 1; cd05581"
                     /db_xref="CDD:270733"
     Site            order(87..91,95,108,110,144,160..161,163,167,169,206,208,
                     210..211,213,223..224,227,263..268,294,300,303)
                     /site_type="active"
                     /db_xref="CDD:270733"
     Site            order(87..90,93,95,108,110,129,144,160..163,166..167,
                     210..211,213,223..224)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270733"
     Site            order(91,167,169,206,208,210,227,263..268,294,300,303)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270733"
     Site            order(223..232,238,260..268)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270733"
     Region          481..587
                     /region_name="PH_PDK1"
                     /note="3-Phosphoinositide dependent protein kinase 1
                     (PDK1) pleckstrin homology (PH) domain; cd01262"
                     /db_xref="CDD:241293"
     Site            order(505,507,512,514,526,535,561)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:241293"
     CDS             1..596
                     /gene="Pdk1"
                     /coded_by="XM_034323620.2:1928..3718"
                     /db_xref="GeneID:117603960"
ORIGIN      
        1 mihaayaiyy ivrvanqalt vspqpavtve gpvskmsppt ndvtngvvap pntlaprvsp
       61 tanpalttin pptpkrnpkd fifgkvigeg sfstvylakd ihsgkeyaik vcdkrhiike
      121 kkteyvkrek evlnmladak hsfvrlfctf qdverlyfvl syakngellp yinkvgsfdi
      181 ectkfysaei lrgleylhgl giihrdlkpe nilldekmhv litdfgsaki lkdpetvsth
      241 ttatddpqqq qqqqyrrerr gsfvgtaqyv spelltdkts rasdlwalgc ivyqmvaglp
      301 pfrsrseymi fqkilkleye ipdgfcelar slvsqllvle pserlgaqde hgagypsira
      361 hpffegvdfe tlheqtpppi ypylpgtseh eelrsqyrvp dhlepglddk qltrllglgi
      421 gedddddddd dddedatier ekvaqqatkv vekprrrtna vatdgnitdl tpeeiknrle
      481 kqrasnqwdv fvegnlilkq gfvnkrkglf arrrmllltt gphlyyvdpv nmvlkgeipw
      541 spelrvepks fkiffvhtpn rtyyledpeg falewcraie ntrvhyyglq esttta
//
LOCUS       XP_034179519             561 aa            linear   INV 03-SEP-2025
DEFINITION  phosphoinositide-dependent kinase 1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034179519
VERSION     XP_034179519.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323628.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179519.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..561
                     /product="phosphoinositide-dependent kinase 1 isoform X4"
                     /calculated_mol_wt=63866
     Region          44..329
                     /region_name="STKc_PDK1"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Phosphoinositide-dependent kinase 1; cd05581"
                     /db_xref="CDD:270733"
     Site            order(52..56,60,73,75,109,125..126,128,132,134,171,173,
                     175..176,178,188..189,192,228..233,259,265,268)
                     /site_type="active"
                     /db_xref="CDD:270733"
     Site            order(52..55,58,60,73,75,94,109,125..128,131..132,
                     175..176,178,188..189)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270733"
     Site            order(56,132,134,171,173,175,192,228..233,259,265,268)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270733"
     Site            order(188..197,203,225..233)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270733"
     Region          446..552
                     /region_name="PH_PDK1"
                     /note="3-Phosphoinositide dependent protein kinase 1
                     (PDK1) pleckstrin homology (PH) domain; cd01262"
                     /db_xref="CDD:241293"
     Site            order(470,472,477,479,491,500,526)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:241293"
     CDS             1..561
                     /gene="Pdk1"
                     /coded_by="XM_034323628.2:500..2185"
                     /db_xref="GeneID:117603960"
ORIGIN      
        1 mspptndvtn gvvappntla prvsptanpa lttinpptpk rnpkdfifgk vigegsfstv
       61 ylakdihsgk eyaikvcdkr hiikekktey vkrekevlnm ladakhsfvr lfctfqdver
      121 lyfvlsyakn gellpyinkv gsfdiectkf ysaeilrgle ylhglgiihr dlkpenilld
      181 ekmhvlitdf gsakilkdpe tvsthttatd dpqqqqqqqy rrerrgsfvg taqyvspell
      241 tdktsrasdl walgcivyqm vaglppfrsr seymifqkil kleyeipdgf celarslvsq
      301 llvlepserl gaqdehgagy psirahpffe gvdfetlheq tpppiypylp gtseheelrs
      361 qyrvpdhlep glddkqltrl lglgigeddd dddddddded atierekvaq qatkvvekpr
      421 rrtnavatdg nitdltpeei knrlekqras nqwdvfvegn lilkqgfvnk rkglfarrrm
      481 lllttgphly yvdpvnmvlk geipwspelr vepksfkiff vhtpnrtyyl edpegfalew
      541 craientrvh yyglqesttt a
//
LOCUS       XP_034179531             213 aa            linear   INV 03-SEP-2025
DEFINITION  aprataxin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034179531
VERSION     XP_034179531.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323640.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179531.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..213
                     /product="aprataxin isoform X1"
                     /calculated_mol_wt=24696
     Region          32..140
                     /region_name="DcpS_C"
                     /note="Scavenger mRNA decapping enzyme C-term binding;
                     pfam11969"
                     /db_xref="CDD:463415"
     Region          145..206
                     /region_name="zf-C2HE"
                     /note="C2HE / C2H2 / C2HC zinc-binding finger; pfam16278"
                     /db_xref="CDD:465082"
     CDS             1..213
                     /gene="Aptx"
                     /coded_by="XM_034323640.2:288..929"
                     /db_xref="GeneID:117603987"
ORIGIN      
        1 mifsrkckei itmkrkpklt ttdatapkkh hwatgllvsm edpelkvked dkivvikdky
       61 pkaqyhylil pkadipslwh ikkenedllf hmakvaddli kehkdseffi gyhavpsmhr
      121 lhlhvistdf nspclktkyh wnsfttpffl hsadvcnqlr ekgelkrlks edsaqhlntp
      181 lkchkcsatp knmpdlkrhl lvhlsdrkgm nid
//
LOCUS       XP_034179539             201 aa            linear   INV 03-SEP-2025
DEFINITION  aprataxin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034179539
VERSION     XP_034179539.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323648.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034179539.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="aprataxin isoform X2"
                     /calculated_mol_wt=23245
     Region          20..128
                     /region_name="DcpS_C"
                     /note="Scavenger mRNA decapping enzyme C-term binding;
                     pfam11969"
                     /db_xref="CDD:463415"
     Region          133..194
                     /region_name="zf-C2HE"
                     /note="C2HE / C2H2 / C2HC zinc-binding finger; pfam16278"
                     /db_xref="CDD:465082"
     CDS             1..201
                     /gene="Aptx"
                     /coded_by="XM_034323648.2:307..912"
                     /db_xref="GeneID:117603987"
ORIGIN      
        1 mkrkpklttt datapkkhhw atgllvsmed pelkvkeddk ivvikdkypk aqyhylilpk
       61 adipslwhik kenedllfhm akvaddlike hkdseffigy havpsmhrlh lhvistdfns
      121 pclktkyhwn sfttpfflhs advcnqlrek gelkrlksed saqhlntplk chkcsatpkn
      181 mpdlkrhllv hlsdrkgmni d
//
LOCUS       XP_076546359             502 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602157 [Osmia lignaria lignaria].
ACCESSION   XP_076546359
VERSION     XP_076546359.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690244.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="uncharacterized protein LOC117602157"
                     /calculated_mol_wt=56445
     Region          1..484
                     /region_name="AFD_class_I"
                     /note="Adenylate forming domain, Class I superfamily;
                     cl17068"
                     /db_xref="CDD:473059"
     Site            order(157,160..165,167..168)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(160,270..271,293..298,376,388,391,402,483)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(160,196..197,243,245..246,249,270..271,293..298,376,
                     388,391,399..402,464)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(196,245..246,249,270,399..401,457,464)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..502
                     /gene="LOC117602157"
                     /coded_by="XM_076690244.1:486..1994"
                     /db_xref="GeneID:117602157"
ORIGIN      
        1 msikcalwmq kqgvksndii vissqsdrei fvpaiaalfi gaifspwdde kdtgkffrtm
       61 tyeifnrfca tklyhrvtdm srhyikllqp kvvfankkss pvvleaaele dhqikvvafe
      121 ncpgtipfad ilkghskgsv anfqcteine ndhtamimfs sgttglpkgi ttthrsllav
      181 veswlgppvh tcmwysclfw lsgplfnmrn iifhtkkiip ppfeeesacr iiekykvewl
      241 lmnttninrl vrynrlheyd fsslkvlyia gspmekvaqd lvkkcfpntd vvqlygmaem
      301 gnliaaqsvn spsgssglva rncevkiidl etgktlgpnq kgeicgksis mmsgyyknpe
      361 atkkvidedg wlhtsdlgyy teegelfvvd rlsdlikfrd hliipseiea vllthpsvle
      421 vavigkphpv dgehplgfvs ivpnkevsak elidlvvskl pdhcrlrggv iflpsfpytp
      481 tgkiwkmklk mlktmyiner tr
//
LOCUS       XP_076546360             845 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117602134 [Osmia lignaria lignaria].
ACCESSION   XP_076546360
VERSION     XP_076546360.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690245.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..845
                     /product="uncharacterized protein LOC117602134"
                     /calculated_mol_wt=96525
     Region          36..107
                     /region_name="Propep_M14"
                     /note="Carboxypeptidase activation peptide; pfam02244"
                     /db_xref="CDD:460505"
     Region          122..410
                     /region_name="M14_CP_A-B_like"
                     /note="Peptidase M14 carboxypeptidase subfamily A/B-like;
                     cd03860"
                     /db_xref="CDD:349433"
     Site            order(178,181,234,250..251,303,355,357,378)
                     /site_type="active"
                     /db_xref="CDD:349433"
     Region          461..534
                     /region_name="Propep_M14"
                     /note="Carboxypeptidase activation peptide; pfam02244"
                     /db_xref="CDD:460505"
     Region          549..837
                     /region_name="M14_CP_A-B_like"
                     /note="Peptidase M14 carboxypeptidase subfamily A/B-like;
                     cd03860"
                     /db_xref="CDD:349433"
     Site            order(605,608,661,677..678,730,782,784,805)
                     /site_type="active"
                     /db_xref="CDD:349433"
     CDS             1..845
                     /gene="LOC117602134"
                     /coded_by="XM_076690245.1:16..2553"
                     /db_xref="GeneID:117602134"
ORIGIN      
        1 mkaysmmktt lllllsilln giratdkmry dgyklykifv pdtsglellk nmqgndgynf
       61 wippkindsa svmispgklq efmeitnktk ieaelmmddv qkyiddenpr aglrgtfdwl
      121 gyhrldviyr wldslatqhp kivtlitggs syegrsikgv klsykegnpg ifieggihar
      181 ewispavvty ilnelilsdd trvrymaesy dwyifpvfnp dgyeythttn rlwrktrrpt
      241 grgcygadpn rnwnfhwaeg gtssnpcsei ypgdkpfset esrtmseyid sihdklfsyi
      301 afhsysqlll ipyghsneri snyndllqig nysinvlskk ygtkykvgni vdviyvasgg
      361 smdwvrgtyn ipvtytyelr dkgrygfilp anqiiptame imdslvamfq eaakrgygss
      421 pvktrqeddr fwvvtmwkll fvvlvalata ekvkfdnykv frvvpqneeh lemlrgleev
      481 sdsysfwkep stvlnyadvm vaphkepefk dlmkqfnipf dvyvpdvqsl idsemppvqp
      541 lagfdlnkyh tldeiyahld dlarsnpgkv qvivggktye grqikgvkls fggnkpgifi
      601 eggiharewi tpasilymln ellysksadv rslaqshdwy ifpvfnpdgy vythttnrlw
      661 rktrkpygil cygsdpnrnw gykwmsggss stpcsetyag saafsdvetk tmsdyiksis
      721 nkfyayisfh sysqllmfpy ghtkehleny daslqigqkt iqalakrygt ryqtgnvaet
      781 iyiasgssvd wvkgtfhkpi tytyelrdtg rhgfvlpadq ikptaeetld slvamfkesk
      841 argye
//
LOCUS       XP_034184986             998 aa            linear   INV 03-SEP-2025
DEFINITION  exonuclease mut-7 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184986
VERSION     XP_034184986.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329095.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184986.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..998
                     /product="exonuclease mut-7 homolog isoform X1"
                     /calculated_mol_wt=116751
     Region          387..577
                     /region_name="mut-7_like_exo"
                     /note="DEDDy 3'-5' exonuclease domain of Caenorhabditis
                     elegans mut-7 and similar proteins; cd06146"
                     /db_xref="CDD:176655"
     Site            order(413..416,472..473,475..477,528..529,562,566)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:176655"
     Site            order(413,415,477,562,566)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176655"
     Site            order(414..416,472..473,475..476,528..529,562,566)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:176655"
     Region          <587..715
                     /region_name="PTZ00441"
                     /note="sporozoite surface protein 2 (SSP2); Provisional"
                     /db_xref="CDD:240420"
     Region          763..998
                     /region_name="Mut7-C"
                     /note="Mut7-C RNAse domain; cl19501"
                     /db_xref="CDD:473177"
     CDS             1..998
                     /gene="Nbr"
                     /coded_by="XM_034329095.2:249..3245"
                     /db_xref="GeneID:117606538"
ORIGIN      
        1 mtntiqkidd sftddlkffs middttkewl tslnhiwqlw kkcdsvsktl ieyfesapnp
       61 ylstirilvn tsdfkymktn sslafaviee yikwlepkkd qykhflvldl klaafqlvns
      121 qnnmefikmv avtyefvehk eeflsiikkl vhekkykkaa qyvvmlqlqd yfgdpeslll
      181 plmlqnklti veevldncpe mqkalvtyld nligpdnamh itldeiimkd nipdvkmstt
      241 qvkpmtklia rfvklynlpp efcpnlnkkr cegalqflih kryvdcslst aswremvqet
      301 iggdeklqlh mirllinakd akeglywakk fdipkeqwpw aityedehie seginegass
      361 srvninsdel tediinchel klarnsikii dnsrsfeefl dtgfngvyiv gidsewkpcf
      421 gtkqtelali qiatdnnvyi ldvttmgnds edlwselali lfenknilkl gfgiaqdmtv
      481 mrdslpalsk vktygpgyld ivhlwqklvn dykfvfphes ddqftkqsls klvelclgnr
      541 lnksdqfsnw eqrplregqi iyaaldaycl leiyevitrq cerldipfpd ictelqhiph
      601 rspkrntkkp aqnqnaiene vpnnnqqqrf knpsqrfrnf qkpmflnrqd ypnrqdhpnr
      661 qdhpnrqvhp nrqdyphrqn ypnkqnhpnr qnhpnkqyfp nrqygnhpnk qdypkkpmhl
      721 hhqkysildl ndepcymtkd yntetpqnqp minkklipah kwrvvcdsml ggltsklrmc
      781 gcdcvhvafd qngerslkla vdenrvlltr nkndlkfsky lpperyymvi adsceeqlre
      841 vlkyfgiyvt qkdifsrcqi cnndefakip ktlmdklvqs fvkltrenny ripsssldsv
      901 henslelpit ndsnsrdnyy vnnameledr twklsvdsvn intcatkyqa riqidkvpin
      961 vlrnlpvfyi ceqcgqvyws gshlertlnh tikdiisk
//
LOCUS       XP_034184996             875 aa            linear   INV 03-SEP-2025
DEFINITION  exonuclease mut-7 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184996
VERSION     XP_034184996.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329105.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184996.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..875
                     /product="exonuclease mut-7 homolog isoform X2"
                     /calculated_mol_wt=102226
     Region          264..454
                     /region_name="mut-7_like_exo"
                     /note="DEDDy 3'-5' exonuclease domain of Caenorhabditis
                     elegans mut-7 and similar proteins; cd06146"
                     /db_xref="CDD:176655"
     Site            order(290..293,349..350,352..354,405..406,439,443)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:176655"
     Site            order(290,292,354,439,443)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:176655"
     Site            order(291..293,349..350,352..353,405..406,439,443)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:176655"
     Region          <464..592
                     /region_name="PTZ00441"
                     /note="sporozoite surface protein 2 (SSP2); Provisional"
                     /db_xref="CDD:240420"
     Region          640..875
                     /region_name="Mut7-C"
                     /note="Mut7-C RNAse domain; cl19501"
                     /db_xref="CDD:473177"
     CDS             1..875
                     /gene="Nbr"
                     /coded_by="XM_034329105.2:248..2875"
                     /db_xref="GeneID:117606538"
ORIGIN      
        1 mefikmvavt yefvehkeef lsiikklvhe kkykkaaqyv vmlqlqdyfg dpeslllplm
       61 lqnkltivee vldncpemqk alvtyldnli gpdnamhitl deiimkdnip dvkmsttqvk
      121 pmtkliarfv klynlppefc pnlnkkrceg alqflihkry vdcslstasw remvqetigg
      181 deklqlhmir llinakdake glywakkfdi pkeqwpwait yedehieseg inegasssrv
      241 ninsdelted iinchelkla rnsikiidns rsfeefldtg fngvyivgid sewkpcfgtk
      301 qtelaliqia tdnnvyildv ttmgndsedl wselalilfe nknilklgfg iaqdmtvmrd
      361 slpalskvkt ygpgyldivh lwqklvndyk fvfphesddq ftkqslsklv elclgnrlnk
      421 sdqfsnweqr plregqiiya aldaycllei yevitrqcer ldipfpdict elqhiphrsp
      481 krntkkpaqn qnaienevpn nnqqqrfknp sqrfrnfqkp mflnrqdypn rqdhpnrqdh
      541 pnrqvhpnrq dyphrqnypn kqnhpnrqnh pnkqyfpnrq ygnhpnkqdy pkkpmhlhhq
      601 kysildlnde pcymtkdynt etpqnqpmin kklipahkwr vvcdsmlggl tsklrmcgcd
      661 cvhvafdqng erslklavde nrvlltrnkn dlkfskylpp eryymviads ceeqlrevlk
      721 yfgiyvtqkd ifsrcqicnn defakipktl mdklvqsfvk ltrennyrip sssldsvhen
      781 slelpitnds nsrdnyyvnn ameledrtwk lsvdsvnint catkyqariq idkvpinvlr
      841 nlpvfyiceq cgqvywsgsh lertlnhtik diisk
//
LOCUS       XP_034185008             897 aa            linear   INV 03-SEP-2025
DEFINITION  swt1 RNA endoribonuclease [Osmia lignaria lignaria].
ACCESSION   XP_034185008
VERSION     XP_034185008.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329117.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185008.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..897
                     /product="swt1 RNA endoribonuclease"
                     /calculated_mol_wt=102830
     Region          6..37
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          <24..>260
                     /region_name="wall_bind_EntB"
                     /note="cell wall-binding protein EntB; NF040676"
                     /db_xref="CDD:468642"
     Region          416..559
                     /region_name="PIN_Swt1-like"
                     /note="VapC-like PIN domain of Saccharomyces cerevisiae
                     Swt1p, human SWT1 and related proteins; cd18727"
                     /db_xref="CDD:350294"
     CDS             1..897
                     /gene="Swt1"
                     /coded_by="XM_034329117.2:253..2946"
                     /db_xref="GeneID:117606548"
ORIGIN      
        1 mmkqklpknw ivlaskthpd rvyyfnvktn qsswekpmed qpkqvtkkie pakkrkkvth
       61 esdaeirptt peydasekei gerkkltakr slrrtqakvn eekvnepkdn kekdtpqmqi
      121 lrekilkkke tgspksskdd tkslatksdr nspvheteid stsntvytpq mkallekmqe
      181 rgsksvsntk kvkenkdsdi kskeekpvtk lrqlkskgrk eigkkakeec dsveatptrq
      241 slkpmkknlg kermeklkkn laatitdgkn lgsscvsiis saihkqrryt nicknaevrl
      301 trlkdklskd atssnkedhh ssspqnkkcm epketlepvs svtvvdelvs rsnrdalyee
      361 mdwepledek ishevqavrt qlctenstnt synipsnmlk ypllveqqek rqvyivvdtn
      421 vflsnieiid lvqettlkty drslivipwt vireldyikd dhgktkpall cykarkavny
      481 inklfssknp rvigqtpedv vknkekfsvd cpddeilqtc lqirdlgksv vllsydinlc
      541 tkamihdivt igrndpleki dylnatnyfn gpsksvnqdr erlglgstsi lstklfiade
      601 miedtksvmr dfltvivske mhtlygdswe kyvivkppwt ivtvlqcaik hwiaavsesf
      661 irraeadlke llqifkdtsg kkslkqvifi lnkcsdlvqm vhvdkyhdlm srvskkvnel
      721 lqkcydyelq indqkvrdei gvendvkeqe rravkafryf eaayvyardm cglaaevvgm
      781 pctfhyntpd pvpavdyvkq iqpelaanvn rllcnlsavi eqvktsridy rtlnnlhetl
      841 vsflpeamps klklnddlap ldvyccvklk eevlktglrq lqelsthfcr fasyrct
//
LOCUS       XP_034185019             814 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606557 [Osmia lignaria lignaria].
ACCESSION   XP_034185019
VERSION     XP_034185019.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329128.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185019.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..814
                     /product="uncharacterized protein LOC117606557"
                     /calculated_mol_wt=90609
     Region          <47..175
                     /region_name="Ebola_NP"
                     /note="Ebola nucleoprotein; pfam05505"
                     /db_xref="CDD:398905"
     CDS             1..814
                     /gene="LOC117606557"
                     /coded_by="XM_034329128.2:542..2986"
                     /db_xref="GeneID:117606557"
ORIGIN      
        1 mgqawckeks tkaqdskspl drvfvrcahr iypglkeegs vlggatqrvt sseigyagsp
       61 prevltrgrs idsvdrpfhp sdwvdvnlev pstprgspvl tnltvdtslh ptstdsnskn
      121 etpippprrr kknkgrplpp kpdeatenss snskgtetne eplyssvksp kssgdeqdee
      181 tgkqrctndg rrtkeiyehk vngtgriiss dvvsgkrtsr ieekksqrss esnhhhhqsi
      241 pendeyerfa snrtskdsst pnreekmkes ssnaraknys tvslpnydel dvarhrtmei
      301 eeadkgkkkp rrpvrsstgs lpaasylspf sektsvrled yipsqgspdn lsayqllqkl
      361 ncgqeeafvk yetasksqdw dfgdigncel nhqqpsspye viirvedeni reqvdedvvd
      421 fsgdnerksp adpdvsnssi csekaeafgk aspvvgeley sevdpnrsae irsqtrhfdp
      481 ptlvdscrvs lahdsstske pffldekkhr saldaiggsc mngesirtst iftrslsies
      541 epnedacetk dlqtglirsi seeslpkeml eeqqldqlfd eklakdvhnk lkksldwktk
      601 tpppspepkt rsepmdldhs tllkvlkeea aeesnlssmt pslteleaal sdmlereecq
      661 egsideknsa esvartnqsl aqeskgknda pvclgqilke rkpatgrkvs fcsweeavaq
      721 sgnrsdweft erpvdldkrt vshpnddprd sigdgvacpm dlppekpsrl nrslddsile
      781 mptppkrrnr slqlakkdal veengsayfn drli
//
LOCUS       XP_034185032             755 aa            linear   INV 03-SEP-2025
DEFINITION  SPG7 matrix AAA peptidase subunit, paraplegin [Osmia lignaria
            lignaria].
ACCESSION   XP_034185032
VERSION     XP_034185032.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329141.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185032.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..755
                     /product="SPG7 matrix AAA peptidase subunit, paraplegin"
                     /calculated_mol_wt=84657
     Region          129..728
                     /region_name="HflB"
                     /note="ATP-dependent Zn proteases [Posttranslational
                     modification, protein turnover, chaperones]; COG0465"
                     /db_xref="CDD:440233"
     CDS             1..755
                     /gene="Spg7"
                     /coded_by="XM_034329141.2:309..2576"
                     /db_xref="GeneID:117606566"
ORIGIN      
        1 mqslikhpkr qfvisrklvg slfkydarky giikatssni kfnsainrlc fplscglytk
       61 vykelnelql flkndfckss klcnprnfst ttsskqsskg ssessedpgk dskrpnefqi
      121 llvkfavgfl valsvyrlis lqptdavgdr inwndfinei llkgqvqeir iyprkvqvvl
      181 krgatykgkl lfsrldlyni pdtkdieeki rlierrigik medgipiryi sfglenilge
      241 llrlalvigl itffarganl knglnnifsd lrkakftlvd pfsgkgvhfn dvaglkeaki
      301 evmefvdylk nperyrklga kvpkgalllg ppgcgktlla kavatesnvp flsmngsefi
      361 eivgglgavr vrdlfaeakk rapsiiyide idaigkkrse gisqfsnses ertlnqllve
      421 mdgmttsedi iilastnrad vldkallrpg rfdrhilidl ptleereqif eyhlkslvle
      481 gspsqyskyl ahltpgfsga eianvcneaa lhaasnsktk venidlmyai dkilggtvkk
      541 sstltpseke vvvyheagha laawllehid plikvtvipr tnkrlgftqy sdsnlklrsv
      601 enlfqnmcvl lggrvaenii fnkistraqn dlkkvtemay rqvqqfgmsp avglisfnke
      661 ytttktkkpy skkmaslmds evrrivteay kttekllldn kdkliilaea llknetltyk
      721 diekligppp fgkkdvmeve elnlnleskv qedlk
//
LOCUS       XP_034185047             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606571 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185047
VERSION     XP_034185047.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329156.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185047.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC117606571 isoform X1"
                     /calculated_mol_wt=54398
     CDS             1..479
                     /gene="LOC117606571"
                     /coded_by="XM_034329156.2:16..1455"
                     /db_xref="GeneID:117606571"
ORIGIN      
        1 mlilrgkilf yrptrprdfv vtersvngyc kkiktpgees vafstlsfvh cqkfqtelfr
       61 rreatfprtm gtmrnnfatv fvllllmlst sqslphgyen sskrnsladd ltsddrtaly
      121 piynclfvdp dlpicdvelm lnsdektter tndqyfdkdl vtrsrrnadv satarvvvya
      181 iegcvparlp fslpscngis lnnivdvrrl rpfvvkgmtq ppyvfpkfny skwirmmarl
      241 hsrvsskkqd ifnvsfqeee ssipkeisae nsledrreel rimpkgsapt hgtrksevde
      301 sressasssv eninndsqit lttksenfss kknikkiekm nkrrgdawkg skdqnegvvr
      361 sfqrrrreir lsgnglmqgi pdeskaqgia gksiaqrfqs ngetdqlnis neqrkhsist
      421 teipgldten rgtksrfddp sfkidmasrk sswrrgmmkd aalgkkhkiy selsasasq
//
LOCUS       XP_034185055             472 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606571 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185055
VERSION     XP_034185055.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329164.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185055.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..472
                     /product="uncharacterized protein LOC117606571 isoform X2"
                     /calculated_mol_wt=53562
     CDS             1..472
                     /gene="LOC117606571"
                     /coded_by="XM_034329164.2:16..1434"
                     /db_xref="GeneID:117606571"
ORIGIN      
        1 mlilrgkilf yrptrprdfv vtersvngyc kkiktpgees vafstlsfvh cqkfqtelfr
       61 rreatfprtm gtmrnnfatv fvllllmlst sqslphgyen sskrnsladd ltsddrtaly
      121 piynclfvdp dlpicdvelm lnsdektter tndqyfdkdl vtrsrrnadv satarvvvya
      181 iegcvparlp fslpscngis lnnivdvrrl rpfvvkgmtq ppyvfpkfny skwirmmarl
      241 hsrvsskkqd eeessipkei saensledrr eelrimpkgs apthgtrkse vdesressas
      301 ssveninnds qitlttksen fsskknikki ekmnkrrgda wkgskdqneg vvrsfqrrrr
      361 eirlsgnglm qgipdeskaq giagksiaqr fqsngetdql nisneqrkhs istteipgld
      421 tenrgtksrf ddpsfkidma srksswrrgm mkdaalgkkh kiyselsasa sq
//
LOCUS       XP_034185066             410 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606571 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185066
VERSION     XP_034185066.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329175.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185066.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="uncharacterized protein LOC117606571 isoform X3"
                     /calculated_mol_wt=46230
     CDS             1..410
                     /gene="LOC117606571"
                     /coded_by="XM_034329175.2:335..1567"
                     /db_xref="GeneID:117606571"
ORIGIN      
        1 mgtmrnnfat vfvllllmls tsqslphgye nsskrnslad dltsddrtal ypiynclfvd
       61 pdlpicdvel mlnsdektte rtndqyfdkd lvtrsrrnad vsatarvvvy aiegcvparl
      121 pfslpscngi slnnivdvrr lrpfvvkgmt qppyvfpkfn yskwirmmar lhsrvsskkq
      181 difnvsfqee essipkeisa ensledrree lrimpkgsap thgtrksevd esressasss
      241 veninndsqi tlttksenfs skknikkiek mnkrrgdawk gskdqnegvv rsfqrrrrei
      301 rlsgnglmqg ipdeskaqgi agksiaqrfq sngetdqlni sneqrkhsis tteipgldte
      361 nrgtksrfdd psfkidmasr ksswrrgmmk daalgkkhki yselsasasq
//
LOCUS       XP_034185077             410 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606571 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185077
VERSION     XP_034185077.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329186.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185077.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="uncharacterized protein LOC117606571 isoform X3"
                     /calculated_mol_wt=46230
     CDS             1..410
                     /gene="LOC117606571"
                     /coded_by="XM_034329186.2:243..1475"
                     /db_xref="GeneID:117606571"
ORIGIN      
        1 mgtmrnnfat vfvllllmls tsqslphgye nsskrnslad dltsddrtal ypiynclfvd
       61 pdlpicdvel mlnsdektte rtndqyfdkd lvtrsrrnad vsatarvvvy aiegcvparl
      121 pfslpscngi slnnivdvrr lrpfvvkgmt qppyvfpkfn yskwirmmar lhsrvsskkq
      181 difnvsfqee essipkeisa ensledrree lrimpkgsap thgtrksevd esressasss
      241 veninndsqi tlttksenfs skknikkiek mnkrrgdawk gskdqnegvv rsfqrrrrei
      301 rlsgnglmqg ipdeskaqgi agksiaqrfq sngetdqlni sneqrkhsis tteipgldte
      361 nrgtksrfdd psfkidmasr ksswrrgmmk daalgkkhki yselsasasq
//
LOCUS       XP_034185086             410 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606571 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185086
VERSION     XP_034185086.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329195.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185086.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="uncharacterized protein LOC117606571 isoform X3"
                     /calculated_mol_wt=46230
     CDS             1..410
                     /gene="LOC117606571"
                     /coded_by="XM_034329195.2:353..1585"
                     /db_xref="GeneID:117606571"
ORIGIN      
        1 mgtmrnnfat vfvllllmls tsqslphgye nsskrnslad dltsddrtal ypiynclfvd
       61 pdlpicdvel mlnsdektte rtndqyfdkd lvtrsrrnad vsatarvvvy aiegcvparl
      121 pfslpscngi slnnivdvrr lrpfvvkgmt qppyvfpkfn yskwirmmar lhsrvsskkq
      181 difnvsfqee essipkeisa ensledrree lrimpkgsap thgtrksevd esressasss
      241 veninndsqi tlttksenfs skknikkiek mnkrrgdawk gskdqnegvv rsfqrrrrei
      301 rlsgnglmqg ipdeskaqgi agksiaqrfq sngetdqlni sneqrkhsis tteipgldte
      361 nrgtksrfdd psfkidmasr ksswrrgmmk daalgkkhki yselsasasq
//
LOCUS       XP_034185096             410 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606571 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185096
VERSION     XP_034185096.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329205.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185096.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..410
                     /product="uncharacterized protein LOC117606571 isoform X3"
                     /calculated_mol_wt=46230
     CDS             1..410
                     /gene="LOC117606571"
                     /coded_by="XM_034329205.2:610..1842"
                     /db_xref="GeneID:117606571"
ORIGIN      
        1 mgtmrnnfat vfvllllmls tsqslphgye nsskrnslad dltsddrtal ypiynclfvd
       61 pdlpicdvel mlnsdektte rtndqyfdkd lvtrsrrnad vsatarvvvy aiegcvparl
      121 pfslpscngi slnnivdvrr lrpfvvkgmt qppyvfpkfn yskwirmmar lhsrvsskkq
      181 difnvsfqee essipkeisa ensledrree lrimpkgsap thgtrksevd esressasss
      241 veninndsqi tlttksenfs skknikkiek mnkrrgdawk gskdqnegvv rsfqrrrrei
      301 rlsgnglmqg ipdeskaqgi agksiaqrfq sngetdqlni sneqrkhsis tteipgldte
      361 nrgtksrfdd psfkidmasr ksswrrgmmk daalgkkhki yselsasasq
//
LOCUS       XP_034185111             282 aa            linear   INV 03-SEP-2025
DEFINITION  phytanoyl-CoA dioxygenase domain containing 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185111
VERSION     XP_034185111.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329220.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185111.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="phytanoyl-CoA dioxygenase domain containing 1"
                     /calculated_mol_wt=32423
     Region          3..274
                     /region_name="PhyH"
                     /note="Ectoine hydroxylase-related dioxygenase,
                     phytanoyl-CoA dioxygenase (PhyH) family [Secondary
                     metabolites biosynthesis, transport and catabolism];
                     COG5285"
                     /db_xref="CDD:444095"
     CDS             1..282
                     /gene="Phyhd1"
                     /coded_by="XM_034329220.2:263..1111"
                     /db_xref="GeneID:117606581"
ORIGIN      
        1 mkdirsqfek ngfvvledff qpeeidelkf cgeeftknlp penerkifns vdsqqskdqy
       61 fldsankinl ffeaealend gtlkvhprvs lnkvghalhw lhptfkkytf dervkeaafq
      121 ldyaepavcq smyiyknpgt gsevvmhqda tylytdpvkl vgfwialeda tqengclwia
      181 pgshqsgvhr rytrnknpds kellsydrpa pcyqlsnfqs vpvskgtcil ihgqvvhfsy
      241 pnksdksrha ytfhvidtqh vsyskenwlq ppsggfpkly rn
//
LOCUS       XP_034180981             634 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604709 [Osmia lignaria lignaria].
ACCESSION   XP_034180981
VERSION     XP_034180981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325090.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..634
                     /product="uncharacterized protein LOC117604709"
                     /calculated_mol_wt=72428
     Region          31..203
                     /region_name="Ins_allergen_rp"
                     /note="Insect allergen related repeat, nitrile-specifier
                     detoxification; pfam06757"
                     /db_xref="CDD:462003"
     Region          249..419
                     /region_name="Ins_allergen_rp"
                     /note="Insect allergen related repeat, nitrile-specifier
                     detoxification; pfam06757"
                     /db_xref="CDD:462003"
     Region          445..617
                     /region_name="Ins_allergen_rp"
                     /note="Insect allergen related repeat, nitrile-specifier
                     detoxification; pfam06757"
                     /db_xref="CDD:462003"
     CDS             1..634
                     /gene="LOC117604709"
                     /coded_by="XM_034325090.2:150..2054"
                     /db_xref="GeneID:117604709"
ORIGIN      
        1 mmkfafalva vlalasplqa ynvprtgsgv laneiqefvd lvptkeimni vlryiatdvd
       61 vqavlkfvhs aefkqlviei eamseirnim nyiqtngldi yqivnhinky ldldslvppt
      121 nvrmvraagg irgmldevka lvpkekirel yrtkmasskv fadlvaqlks pefqnvanql
      181 cnnatfkhvl veakkaqvdi dglarlletv lgltlpckpm kfslvalsam vafatvncyh
      241 lpafgegply ediqyfidmi pmdevmrviv qytvkdsefq qllkymktdd ckqmvneiea
      301 ipefrtfasy mdkhgiymyd alnkmnkvig lpsfqpilvk keitggltgl fndvkelisy
      361 ddiihgyvyk mrtsqdfrdf vaelkseghq nfvnalyknq rylafratlv skglditlie
      421 dviytvlgie fpvldttnal ydnqlskdiq dfidlidmdk vikiisnyld ddqiqeslry
      481 frseefhvlv rkvealkpyq nlvqylhnag lnihgliqki hklfgmedyv ppsgnyfsff
      541 vnrggmkgli ddilaslpvd kfkamfeekm ktspdfqnfv kqvqskefee iidtvysepi
      601 flemrqkaid igldiqrarq lfeeigillp rpsy
//
LOCUS       XP_034185769             682 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606876 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185769
VERSION     XP_034185769.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329878.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..682
                     /product="uncharacterized protein LOC117606876 isoform X1"
                     /calculated_mol_wt=71234
     Site            order(224..226,228,248,254,267,269..270,273..274,276..278,
                     280..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Site            order(224,227,270,273..274,277)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          240..278
                     /region_name="Homeobox_KN"
                     /note="Homeobox KN domain; pfam05920"
                     /db_xref="CDD:428673"
     Region          406..422
                     /region_name="IRO"
                     /note="Motif in Iroquois-class homeodomain proteins
                     (only). Unknown function; smart00548"
                     /db_xref="CDD:214716"
     CDS             1..682
                     /gene="LOC117606876"
                     /coded_by="XM_034329878.2:636..2684"
                     /db_xref="GeneID:117606876"
ORIGIN      
        1 mdisedcweh vgllqevsgt eppppqlapd fliqqqellv sggqpttats pamssggals
       61 pgalspssta tttgaaaaaa sggastpvgg atgpgcceng rpmmtdpvtg qtvcscqyds
      121 aarlalgayp rlaptatsys syptptpstt dqgpypsigm dssafypplg npyglkdttg
      181 mgmtadmgaa wgtaalqpat tgyypydptl aaygygagyd laarrknatr estatlkawl
      241 nehkknpypt kgekimlaii tkmtltqvst wfanarrrlk kenkmtwepk nktdddddav
      301 ltdsednkek ddltgdnqgd rvgeearrgl eenepmrhvk aehlqhdkdl deeddldlee
      361 dprrgehpfh hamqhhhhhh qgytgedhlk degiksdcsa agvpipatkp kiwsladtaa
      421 cktppppshp hhqqyhhlhh qqhyaqqqqq qqqqqhhltn qghhhhsqqp wlgsgggggt
      481 aggggggggg nlssfalpss asmspsaaat apyssaatry ggflssssgg qlhynpnsss
      541 gssssasssa aagfpevgtd tppqtppnmk vatpngviqa ppggyctggn gnatsngnpn
      601 pygashpaag ylstssgsas sassfssrlq asphkdfspa aqnsilhqhq ttaslpptea
      661 ttafkpfykg sqsmgsgfvs pv
//
LOCUS       XP_034185777             682 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606876 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185777
VERSION     XP_034185777.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329886.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..682
                     /product="uncharacterized protein LOC117606876 isoform X1"
                     /calculated_mol_wt=71234
     Site            order(224..226,228,248,254,267,269..270,273..274,276..278,
                     280..281)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Site            order(224,227,270,273..274,277)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          240..278
                     /region_name="Homeobox_KN"
                     /note="Homeobox KN domain; pfam05920"
                     /db_xref="CDD:428673"
     Region          406..422
                     /region_name="IRO"
                     /note="Motif in Iroquois-class homeodomain proteins
                     (only). Unknown function; smart00548"
                     /db_xref="CDD:214716"
     CDS             1..682
                     /gene="LOC117606876"
                     /coded_by="XM_034329886.2:632..2680"
                     /db_xref="GeneID:117606876"
ORIGIN      
        1 mdisedcweh vgllqevsgt eppppqlapd fliqqqellv sggqpttats pamssggals
       61 pgalspssta tttgaaaaaa sggastpvgg atgpgcceng rpmmtdpvtg qtvcscqyds
      121 aarlalgayp rlaptatsys syptptpstt dqgpypsigm dssafypplg npyglkdttg
      181 mgmtadmgaa wgtaalqpat tgyypydptl aaygygagyd laarrknatr estatlkawl
      241 nehkknpypt kgekimlaii tkmtltqvst wfanarrrlk kenkmtwepk nktdddddav
      301 ltdsednkek ddltgdnqgd rvgeearrgl eenepmrhvk aehlqhdkdl deeddldlee
      361 dprrgehpfh hamqhhhhhh qgytgedhlk degiksdcsa agvpipatkp kiwsladtaa
      421 cktppppshp hhqqyhhlhh qqhyaqqqqq qqqqqhhltn qghhhhsqqp wlgsgggggt
      481 aggggggggg nlssfalpss asmspsaaat apyssaatry ggflssssgg qlhynpnsss
      541 gssssasssa aagfpevgtd tppqtppnmk vatpngviqa ppggyctggn gnatsngnpn
      601 pygashpaag ylstssgsas sassfssrlq asphkdfspa aqnsilhqhq ttaslpptea
      661 ttafkpfykg sqsmgsgfvs pv
//
LOCUS       XP_034185796             661 aa            linear   INV 03-SEP-2025
DEFINITION  homeobox protein araucan isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034185796
VERSION     XP_034185796.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329905.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="homeobox protein araucan isoform X2"
                     /calculated_mol_wt=68814
     Site            order(203..205,207,227,233,246,248..249,252..253,255..257,
                     259..260)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Site            order(203,206,249,252..253,256)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          219..257
                     /region_name="Homeobox_KN"
                     /note="Homeobox KN domain; pfam05920"
                     /db_xref="CDD:428673"
     Region          385..401
                     /region_name="IRO"
                     /note="Motif in Iroquois-class homeodomain proteins
                     (only). Unknown function; smart00548"
                     /db_xref="CDD:214716"
     CDS             1..661
                     /gene="LOC117606876"
                     /coded_by="XM_034329905.2:748..2733"
                     /db_xref="GeneID:117606876"
ORIGIN      
        1 msayaqfgys ypsasqllvs ggqpttatsp amssggalsp galspsstat ttgaaaaaas
       61 ggastpvgga tgpgccengr pmmtdpvtgq tvcscqydsa arlalgaypr laptatsyss
      121 yptptpsttd qgpypsigmd ssafypplgn pyglkdttgm gmtadmgaaw gtaalqpatt
      181 gyypydptla aygygagydl aarrknatre statlkawln ehkknpyptk gekimlaiit
      241 kmtltqvstw fanarrrlkk enkmtwepkn ktdddddavl tdsednkekd dltgdnqgdr
      301 vgeearrgle enepmrhvka ehlqhdkdld eeddldleed prrgehpfhh amqhhhhhhq
      361 gytgedhlkd egiksdcsaa gvpipatkpk iwsladtaac ktppppshph hqqyhhlhhq
      421 qhyaqqqqqq qqqqhhltnq ghhhhsqqpw lgsgggggta gggggggggn lssfalpssa
      481 smspsaaata pyssaatryg gflssssggq lhynpnsssg ssssasssaa agfpevgtdt
      541 ppqtppnmkv atpngviqap pggyctggng natsngnpnp ygashpaagy lstssgsass
      601 assfssrlqa sphkdfspaa qnsilhqhqt taslppteat tafkpfykgs qsmgsgfvsp
      661 v
//
LOCUS       XP_076546361             391 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat domain-containing protein SOWAHC isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546361
VERSION     XP_076546361.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690246.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..391
                     /product="ankyrin repeat domain-containing protein SOWAHC
                     isoform X1"
                     /calculated_mol_wt=42954
     Region          58..124
                     /region_name="CSB_WHD"
                     /note="winged-helix domain (WHD) found in cockayne
                     syndrome group B (CSB) and similar proteins; cd22254"
                     /db_xref="CDD:439329"
     Region          <315..369
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..391
                     /gene="LOC117606896"
                     /coded_by="XM_076690246.1:324..1499"
                     /db_xref="GeneID:117606896"
ORIGIN      
        1 massgvrqqa piltgtlfav geankknsyl paerwiaars asaavssdkm atpselsiee
       61 irnyllengg tarnhdvvkh fkkfltdpet rvearnrfke yvntlatikn eegekylvlk
      121 kkyrqnsldl sspeqsespi atpdistpvs plrlpppyrp pppapqspss nngssqpees
      181 csnfvleeaa ingekndvdr tetglstssp pvpprrksqd kikienkenv drnkggseav
      241 ikedeavvvn pgsteqlsfr ermqrfnrma setdlqgrpn gaitptkkrs dkgadeddsa
      301 svasqqldgk srewlvraaq gdyqalakla aeeprlarlk dptstalhwa akhgdenivk
      361 liagtykdyi ksvnettvst leeniiyipl l
//
LOCUS       XP_076546362             390 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat domain-containing protein SOWAHC isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546362
VERSION     XP_076546362.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690247.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="ankyrin repeat domain-containing protein SOWAHC
                     isoform X2"
                     /calculated_mol_wt=42826
     Region          58..124
                     /region_name="CSB_WHD"
                     /note="winged-helix domain (WHD) found in cockayne
                     syndrome group B (CSB) and similar proteins; cd22254"
                     /db_xref="CDD:439329"
     Region          <314..368
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..390
                     /gene="LOC117606896"
                     /coded_by="XM_076690247.1:324..1496"
                     /db_xref="GeneID:117606896"
ORIGIN      
        1 massgvrqqa piltgtlfav geankknsyl paerwiaars asaavssdkm atpselsiee
       61 irnyllengg tarnhdvvkh fkkfltdpet rvearnrfke yvntlatikn eegekylvlk
      121 kkyrqnsldl sspeqsespi atpdistpvs plrlpppyrp pppapqspss nngssqpees
      181 csnfvleeaa ingekndvdr tetglstssp pvpprrksqd kikienkenv drnkggseav
      241 ikedeavvvn pgsteqlsfr ermqrfnrma setdlqgrpn gaitptkkrs dkgadeddsa
      301 svasqldgks rewlvraaqg dyqalaklaa eeprlarlkd ptstalhwaa khgdenivkl
      361 iagtykdyik svnettvstl eeniiyipll
//
LOCUS       XP_076546363             341 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat domain-containing protein SOWAHC isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546363
VERSION     XP_076546363.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690248.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="ankyrin repeat domain-containing protein SOWAHC
                     isoform X3"
                     /calculated_mol_wt=37293
     Region          58..124
                     /region_name="CSB_WHD"
                     /note="winged-helix domain (WHD) found in cockayne
                     syndrome group B (CSB) and similar proteins; cd22254"
                     /db_xref="CDD:439329"
     Region          <265..319
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..341
                     /gene="LOC117606896"
                     /coded_by="XM_076690248.1:325..1350"
                     /db_xref="GeneID:117606896"
ORIGIN      
        1 massgvrqqa piltgtlfav geankknsyl paerwiaars asaavssdkm atpselsiee
       61 irnyllengg tarnhdvvkh fkkfltdpet rvearnrfke yvntlatikn eegekylvlk
      121 kkyrqnsldl sspeqsespi atpdistpvs plrlpppyrp pppapqspss nngssqpees
      181 csnfvleeaa ingekndvdr tetglstssp pvpprrksqd kikienkenv drnkggseav
      241 ikgadeddsa svasqqldgk srewlvraaq gdyqalakla aeeprlarlk dptstalhwa
      301 akhgdenivk liagtykdyi ksvnettvst leeniiyipl l
//
LOCUS       XP_076546364             340 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat domain-containing protein SOWAHC isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546364
VERSION     XP_076546364.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690249.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="ankyrin repeat domain-containing protein SOWAHC
                     isoform X4"
                     /calculated_mol_wt=37165
     Region          58..124
                     /region_name="CSB_WHD"
                     /note="winged-helix domain (WHD) found in cockayne
                     syndrome group B (CSB) and similar proteins; cd22254"
                     /db_xref="CDD:439329"
     Region          <264..318
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..340
                     /gene="LOC117606896"
                     /coded_by="XM_076690249.1:325..1347"
                     /db_xref="GeneID:117606896"
ORIGIN      
        1 massgvrqqa piltgtlfav geankknsyl paerwiaars asaavssdkm atpselsiee
       61 irnyllengg tarnhdvvkh fkkfltdpet rvearnrfke yvntlatikn eegekylvlk
      121 kkyrqnsldl sspeqsespi atpdistpvs plrlpppyrp pppapqspss nngssqpees
      181 csnfvleeaa ingekndvdr tetglstssp pvpprrksqd kikienkenv drnkggseav
      241 ikgadeddsa svasqldgks rewlvraaqg dyqalaklaa eeprlarlkd ptstalhwaa
      301 khgdenivkl iagtykdyik svnettvstl eeniiyipll
//
LOCUS       XP_076546365             203 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305243 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546365
VERSION     XP_076546365.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690250.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..203
                     /product="uncharacterized protein LOC143305243 isoform X1"
                     /calculated_mol_wt=22559
     CDS             1..203
                     /gene="LOC143305243"
                     /coded_by="XM_076690250.1:30..641"
                     /db_xref="GeneID:143305243"
ORIGIN      
        1 mqfdhenifn llvqvyganq eirdysgkka rqylvsqeaa vsqdtfrkik arkkhaekdl
       61 gflrigslnv rvkrtteafs qflgvatssa ssnhekihkt wgsadnvqvd qkvmpppkya
      121 pikkrrsrra qdfsasrdhq vasqpstpll qgkvsrssns ihrrptstta vnsnttprtq
      181 pnndsdsdta cgfdsawrgs aql
//
LOCUS       XP_076546366             196 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305243 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546366
VERSION     XP_076546366.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690251.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="uncharacterized protein LOC143305243 isoform X2"
                     /calculated_mol_wt=21673
     CDS             1..196
                     /gene="LOC143305243"
                     /coded_by="XM_076690251.1:82..672"
                     /db_xref="GeneID:143305243"
ORIGIN      
        1 mvrikryait verrpdstws lkrplsvrtp saglllplrs eikarkkhae kdlgflrigs
       61 lnvrvkrtte afsqflgvat ssassnheki hktwgsadnv qvdqkvmppp kyapikkrrs
      121 rraqdfsasr dhqvasqpst pllqgkvsrs snsihrrpts ttavnsnttp rtqpnndsds
      181 dtacgfdsaw rgsaql
//
LOCUS       XP_076546367             187 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305243 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546367
VERSION     XP_076546367.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690252.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="uncharacterized protein LOC143305243 isoform X3"
                     /calculated_mol_wt=20610
     CDS             1..187
                     /gene="LOC143305243"
                     /coded_by="XM_076690252.1:82..645"
                     /db_xref="GeneID:143305243"
ORIGIN      
        1 mvrikryait verrpdstws lkrplsvrtp saglllplrs ekdlgflrig slnvrvkrtt
       61 eafsqflgva tssassnhek ihktwgsadn vqvdqkvmpp pkyapikkrr srraqdfsas
      121 rdhqvasqps tpllqgkvsr ssnsihrrpt sttavnsntt prtqpnndsd sdtacgfdsa
      181 wrgsaql
//
LOCUS       XP_034181008             287 aa            linear   INV 03-SEP-2025
DEFINITION  rhythmically expressed gene 2 protein [Osmia lignaria lignaria].
ACCESSION   XP_034181008
VERSION     XP_034181008.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325117.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181008.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="rhythmically expressed gene 2 protein"
                     /calculated_mol_wt=32677
     Region          40..247
                     /region_name="DREG-2"
                     /note="REG-2-like, HAD superfamily (subfamily IA)
                     hydrolase; TIGR02252"
                     /db_xref="CDD:274056"
     CDS             1..287
                     /gene="LOC117604719"
                     /coded_by="XM_034325117.2:1..864"
                     /db_xref="GeneID:117604719"
ORIGIN      
        1 mncpclktgk felsslssgg agdvekllqd smmikfirpr litfdvtgtl lmtkleehyv
       61 digsqhglsv dagklarsfk nsfhklsveh piygkhtgig wenwwrkivh hvfkdqhsyv
      121 sdatldkvan slircygtsm cwhkypgtie lleylrkkgl iigvisnfde rleailedtr
      181 irfyfsfvlt sydfgmekph tsifdealrl tkerhsidva pqeaihigds vsndyngakn
      241 anwnallikr dddvtgdkei paenifrnle dlrlyfstll nsdakri
//
LOCUS       XP_076546368             367 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604724 [Osmia lignaria lignaria].
ACCESSION   XP_076546368
VERSION     XP_076546368.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690253.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 5% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..367
                     /product="uncharacterized protein LOC117604724"
                     /calculated_mol_wt=42041
     Region          106..340
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..367
                     /gene="LOC117604724"
                     /coded_by="XM_076690253.1:1..1104"
                     /db_xref="GeneID:117604724"
ORIGIN      
        1 mivhriavtc ikitlffafv flpristcwd fgavnlpkdi figkfavwrn raflsisrte
       61 rnetlvnrpi lyeaiwpesn fpvvkrsklf ssnlhnklin dgclrsivel didtrgrlwl
      121 levpqnhdcs ariiiynlrr nnqlvsstdl lnvptknlra lavdssgska ylgdpgdesi
      181 iafipeeerw wrimmihgpe vprvvstdia isrknsalyl sgsntlhlfs inleelktsi
      241 ckyifeqsse wlfctttyqk srnvtvvwhg tkmgassgli cdfkdglhyf mssekasvrw
      301 dtklplkaey htilvqdehc pcitdyamds qknlwglins ecpftletps klhlksrtik
      361 ilkypif
//
LOCUS       XP_034189061             373 aa            linear   INV 03-SEP-2025
DEFINITION  L-dopachrome tautomerase yellow-g2 [Osmia lignaria lignaria].
ACCESSION   XP_034189061
VERSION     XP_034189061.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333170.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 4% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="L-dopachrome tautomerase yellow-g2"
                     /calculated_mol_wt=41365
     Region          122..370
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..373
                     /gene="yellow-g2"
                     /coded_by="XM_034333170.2:1..1122"
                     /db_xref="GeneID:117608283"
ORIGIN      
        1 mffkekflsa salivsllvv dvvatfditl nsnvqwtagt fqwpcpttkn mfkssgryip
       61 knviatraai fnddaivalp rykpgvpatl akiqkdeasc eatlvpypcw slqeegtcta
      121 lqnvvdlyld prnilwvldt gvvntldqpl rncppkvlai nvstgklvkt veltglttst
      181 srlqyvvady spdgrvfiyv sdaasrailv ydvtsgrgyr vvlpqavtig ctrrdvlyla
      241 llrrsdgstc lvftylsssr mfsvrtehlr ngsangrihd lgqkakkmif lgtdngsalf
      301 fryegepdiy rwdataqfnp qcfqlvytsn ecflathvva dykrgrmrvl esnfpdymqd
      361 tvgcganqal nlm
//
LOCUS       XP_034186566            1290 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607232 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186566
VERSION     XP_034186566.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330675.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186566.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1290
                     /product="uncharacterized protein LOC117607232 isoform X1"
                     /calculated_mol_wt=119643
     Region          470..>1175
                     /region_name="FhaB"
                     /note="Large exoprotein involved in heme utilization or
                     adhesion [Intracellular trafficking, secretion, and
                     vesicular transport]; COG3210"
                     /db_xref="CDD:442443"
     CDS             1..1290
                     /gene="LOC117607232"
                     /coded_by="XM_034330675.2:157..4029"
                     /db_xref="GeneID:117607232"
ORIGIN      
        1 mrllkcfvpi vlvllvenss asggllglln hvkkehpavf gggsavadak assqafggnn
       61 aaasadakay sqgsgiatan adasatagas gllghlthig dhigghvkqi lgipqqgssa
      121 qansntftsd rdlnlgpigi kshhsfstsn akatgnglas agatansqas ggasakadas
      181 aiagaqgyag slgpnynsnt nnygdgydsy psndqqygsy ggsqsganaa ananayagaa
      241 snantnsnvn ldiypsggak tnvnsngngl paiyyhgner adanaitgan sislgqptlt
      301 vspaggydye iekfppayhp hprphhqpqp hyqphphyqs hpqhqpsviv rpyqpparnd
      361 etvipivivd gpphgrpmph ygprpvphyg prpephygpr pqygpkpkpe pieiivieep
      421 esprqgqgfg ngnananana maiananaat ngnyqvpsgs hqqpqyysss apsgpigann
      481 pflsssgant nvgantngga ypgqgqgtgh yqtgpsapig gnnpflsgsg ngnaganaga
      541 ianagatana gaianagatg gsqapigann pflngalpsg gnygsntgan agatggsqyt
      601 mknipttypg agnygapssg vagsganaga nagsqntmkn ipttypgagn ygapssgvag
      661 sganaganag aiagsqnvik gvptmypsgg nygapssgaa gsgavanaga naganaagsg
      721 gynqpikttp ltvpaspegs yyngasgvng qsgsnsvfen paansgansg tggygaptyg
      781 tsgpaygsgs sganadskas alanagssgs gfggagntgf srtggygqgg anaggsgsal
      841 ggagstgfsg aggygqggan aganskvnag gsglgfggag stgfsgtggy gqgganaggs
      901 gsalggagst gfsgaggygq gganagansk vnaggsgsgf ggagstgfsg aggygqggak
      961 vgssgsalgg agstgfsgtg gygqgganag anskvnaggs gsgfggagst gfsgtggygq
     1021 gganaggsgs alggagstgf sgaggyeqsg akvgssgsal ggagstgfsg aggygqggak
     1081 vessgsvlgg agstgfsgtg gygqgganag ansnanagns gsgvgtpikg lpsygstgpg
     1141 yntglsggaa gasananava nagstgsagn lggnagsdgn syggvgssgf nggggassva
     1201 gakadatata ggygqgsssa sstansssfa sggsasatss akssafgsgn asanakavas
     1261 ssanagsggs sassnaeaki dtrsmlvfsq
//
LOCUS       XP_034186617            1240 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607232 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186617
VERSION     XP_034186617.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330726.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186617.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1240
                     /product="uncharacterized protein LOC117607232 isoform X2"
                     /calculated_mol_wt=115340
     Region          470..>1175
                     /region_name="FhaB"
                     /note="Large exoprotein involved in heme utilization or
                     adhesion [Intracellular trafficking, secretion, and
                     vesicular transport]; COG3210"
                     /db_xref="CDD:442443"
     CDS             1..1240
                     /gene="LOC117607232"
                     /coded_by="XM_034330726.2:157..3879"
                     /db_xref="GeneID:117607232"
ORIGIN      
        1 mrllkcfvpi vlvllvenss asggllglln hvkkehpavf gggsavadak assqafggnn
       61 aaasadakay sqgsgiatan adasatagas gllghlthig dhigghvkqi lgipqqgssa
      121 qansntftsd rdlnlgpigi kshhsfstsn akatgnglas agatansqas ggasakadas
      181 aiagaqgyag slgpnynsnt nnygdgydsy psndqqygsy ggsqsganaa ananayagaa
      241 snantnsnvn ldiypsggak tnvnsngngl paiyyhgner adanaitgan sislgqptlt
      301 vspaggydye iekfppayhp hprphhqpqp hyqphphyqs hpqhqpsviv rpyqpparnd
      361 etvipivivd gpphgrpmph ygprpvphyg prpephygpr pqygpkpkpe pieiivieep
      421 esprqgqgfg ngnananana maiananaat ngnyqvpsgs hqqpqyysss apsgpigann
      481 pflsssgant nvgantngga ypgqgqgtgh yqtgpsapig gnnpflsgsg ngnaganaga
      541 ianagatana gaianagatg gsqapigann pflngalpsg gnygsntgan agatggsqyt
      601 mknipttypg agnygapssg vagsganaga nagsqntmkn ipttypgagn ygapssgvag
      661 sganaganag aiagsqnvik gvptmypsgg nygapssgaa gsgavanaga naganaagsg
      721 gynqpikttp ltvpaspegs yyngasgvng qsgsnsvfen paansgansg tggygaptyg
      781 tsgpaygsgs sganadskas alanagssgs gfggagntgf srtggygqgg anaggsgsal
      841 ggagstgfsg aggygqggan aganskvnag gsglgfggag stgfsgtggy gqgganaggs
      901 gsalggagst gfsgaggygq gganagansk vnaggsgsgf ggagstgfsg aggygqggak
      961 vgssgsalgg agstgfsgtg gygqgganag anskvnaggs gsgfggagst gfsgtggygq
     1021 gganaggsgs alggagstgf sgaggyeqsg akvgssgsal ggagstgfsg aggygqggak
     1081 vessgsvlgg agstgfsgtg gygqgganag ansnanagns gsgvgtpikg lpsygstgpg
     1141 yntglsggaa gasananava nagstgsagn lggnagsdgn syggvgssgf nggggassva
     1201 gakadatata ggygqemsgs akasassfae amqsglilik
//
LOCUS       XP_034186727            1049 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607232 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186727
VERSION     XP_034186727.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330836.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186727.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1049
                     /product="uncharacterized protein LOC117607232 isoform X3"
                     /calculated_mol_wt=93675
     Region          <156..940
                     /region_name="FhaB"
                     /note="Large exoprotein involved in heme utilization or
                     adhesion [Intracellular trafficking, secretion, and
                     vesicular transport]; COG3210"
                     /db_xref="CDD:442443"
     CDS             1..1049
                     /gene="LOC117607232"
                     /coded_by="XM_034330836.2:155..3304"
                     /db_xref="GeneID:117607232"
ORIGIN      
        1 mrllkcfvpi vlvllvenss asggllglln hvkkehpavf gggsavadak assqafggnn
       61 aaasadakay sqgsgiatan adasatagas gllghlthig dhigghvkqi lgipqqgssa
      121 qansntftsd rdlnlgpigi kshhsfstsn akatgnglas agatansqas ggasakadas
      181 aiagaqgfgn gnanananam aiananaatn gnyqvpsgsh qqpqyysssa psgpigannp
      241 flsssgantn vgantnggay pgqgqgtghy qtgpsapigg nnpflsgsgn gnaganagai
      301 anagatanag aianagatgg sqapigannp flngalpsgg nygsntgana gatggsqytm
      361 knipttypga gnygapssgv agsganagan agsqntmkni pttypgagny gapssgvags
      421 ganaganaga iagsqnvikg vptmypsggn ygapssgaag sgavanagan aganaagsgg
      481 ynqpikttpl tvpaspegsy yngasgvngq sgsnsvfenp aansgansgt ggygaptygt
      541 sgpaygsgss ganadskasa lanagssgsg fggagntgfs rtggygqgga naggsgsalg
      601 gagstgfsga ggygqggana ganskvnagg sglgfggags tgfsgtggyg qgganaggsg
      661 salggagstg fsgaggygqg ganaganskv naggsgsgfg gagstgfsga ggygqggakv
      721 gssgsalgga gstgfsgtgg ygqgganaga nskvnaggsg sgfggagstg fsgtggygqg
      781 ganaggsgsa lggagstgfs gaggyeqsga kvgssgsalg gagstgfsga ggygqggakv
      841 essgsvlgga gstgfsgtgg ygqgganaga nsnanagnsg sgvgtpikgl psygstgpgy
      901 ntglsggaag asananavan agstgsagnl ggnagsdgns yggvgssgfn ggggassvag
      961 akadatatag gygqgsssas stansssfas ggsasatssa kssafgsgna sanakavass
     1021 sanagsggss assnaeakid trsmlvfsq
//
LOCUS       XP_034186738             642 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186738
VERSION     XP_034186738.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330847.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..642
                     /product="glutaminase isoform X1"
                     /calculated_mol_wt=72425
     Region          86..177
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          199..487
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          522..604
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          522..548
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(523..524,527..529,531..532,536,539,548,550,552,
                     556..557,560..562,564..565,569,572,582,584,586,590..591,
                     594..596,598..599,603)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          550..582
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..642
                     /gene="GLS"
                     /coded_by="XM_034330847.2:144..2072"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mnreaivrga rmlkivkags nglrrlhtss krraavpask klgsvkylda afgsaleler
       61 aqtrdysfih dshymytrdq dqatnaedvl fdmfkneetg llpvgkflaa lrttglrndd
      121 prlqeftdnl rkehlksggh egvshetqkl sreqfrriis pnivlisraf rhqfiipdws
      181 gftkhiedfy wkcksnsegk vasyipqlar mnpdywgvsv ctidgqrfsi gdtsipftlq
      241 scskpltyai aldrlgqevv hqyvgqepsg rnfnelvldy nkkphnpmin agailvcsll
      301 kslikpemtl aekfdftmny fkrlaggenl gfnnavflse reaadrnyal gfymrehdcy
      361 pdksnlreim dfyfqccsme ancdtmavma atlanggicp iteekvlkpd svrdvlslmh
      421 scgmydysgq fafkvgipak sgvsgsllvv ipnvmgictw sppldplgns crgvqfceel
      481 vtefnfhsty ydhrydnlkh atnkkdprrh kyetkglsiv nllfsaasgd vtamrrhrls
      541 gmdmtlsdyd grtalhlaas eghldcvefl leqcgvpdep kdrwgkrpid eaesfghmqv
      601 veylnnyala rkteteneen evkeentdsr evvetagrtp lp
//
LOCUS       XP_034186748             636 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186748
VERSION     XP_034186748.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330857.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="glutaminase isoform X2"
                     /calculated_mol_wt=71659
     Region          86..177
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          199..487
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          516..598
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          516..542
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(517..518,521..523,525..526,530,533,542,544,546,
                     550..551,554..556,558..559,563,566,576,578,580,584..585,
                     588..590,592..593,597)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          544..576
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..636
                     /gene="GLS"
                     /coded_by="XM_034330857.2:144..2054"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mnreaivrga rmlkivkags nglrrlhtss krraavpask klgsvkylda afgsaleler
       61 aqtrdysfih dshymytrdq dqatnaedvl fdmfkneetg llpvgkflaa lrttglrndd
      121 prlqeftdnl rkehlksggh egvshetqkl sreqfrriis pnivlisraf rhqfiipdws
      181 gftkhiedfy wkcksnsegk vasyipqlar mnpdywgvsv ctidgqrfsi gdtsipftlq
      241 scskpltyai aldrlgqevv hqyvgqepsg rnfnelvldy nkkphnpmin agailvcsll
      301 kslikpemtl aekfdftmny fkrlaggenl gfnnavflse reaadrnyal gfymrehdcy
      361 pdksnlreim dfyfqccsme ancdtmavma atlanggicp iteekvlkpd svrdvlslmh
      421 scgmydysgq fafkvgipak sgvsgsllvv ipnvmgictw sppldplgns crgvqfceel
      481 vtefnfhryd nlkhatnkkd prrhkyetkg lsivnllfsa asgdvtamrr hrlsgmdmtl
      541 sdydgrtalh laaseghldc veflleqcgv pdepkdrwgk rpideaesfg hmqvveylnn
      601 yalarktete neenevkeen tdsrevveta grtplp
//
LOCUS       XP_034186757             630 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186757
VERSION     XP_034186757.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330866.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="glutaminase isoform X3"
                     /calculated_mol_wt=70866
     Region          74..165
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          187..475
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          510..592
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          510..536
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(511..512,515..517,519..520,524,527,536,538,540,
                     544..545,548..550,552..553,557,560,570,572,574,578..579,
                     582..584,586..587,591)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          538..570
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..630
                     /gene="GLS"
                     /coded_by="XM_034330866.2:145..2037"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mnreaivrga rmlkivkags nglrrlhtss krraavpask klgsvkylda afgsaleler
       61 aqtrtrdqdq atnaedvlfd mfkneetgll pvgkflaalr ttglrnddpr lqeftdnlrk
      121 ehlksggheg vshetqklsr eqfrriispn ivlisrafrh qfiipdwsgf tkhiedfywk
      181 cksnsegkva syipqlarmn pdywgvsvct idgqrfsigd tsipftlqsc skpltyaial
      241 drlgqevvhq yvgqepsgrn fnelvldynk kphnpminag ailvcsllks likpemtlae
      301 kfdftmnyfk rlaggenlgf nnavflsere aadrnyalgf ymrehdcypd ksnlreimdf
      361 yfqccsmean cdtmavmaat langgicpit eekvlkpdsv rdvlslmhsc gmydysgqfa
      421 fkvgipaksg vsgsllvvip nvmgictwsp pldplgnscr gvqfceelvt efnfhstyyd
      481 hrydnlkhat nkkdprrhky etkglsivnl lfsaasgdvt amrrhrlsgm dmtlsdydgr
      541 talhlaaseg hldcveflle qcgvpdepkd rwgkrpidea esfghmqvve ylnnyalark
      601 teteneenev keentdsrev vetagrtplp
//
LOCUS       XP_034186766             629 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034186766
VERSION     XP_034186766.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330875.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186766.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="glutaminase isoform X4"
                     /calculated_mol_wt=71319
     Region          73..164
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          186..474
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          509..591
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          509..535
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(510..511,514..516,518..519,523,526,535,537,539,
                     543..544,547..549,551..552,556,559,569,571,573,577..578,
                     581..583,585..586,590)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          537..569
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..629
                     /gene="GLS"
                     /coded_by="XM_034330875.2:567..2456"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mkdksqsivv sdsemnneys denpemmpak fsedysirtk iactmwlekq gyktrrmlar
       61 flstrdqdqa tnaedvlfdm fkneetgllp vgkflaalrt tglrnddprl qeftdnlrke
      121 hlksgghegv shetqklsre qfrriispni vlisrafrhq fiipdwsgft khiedfywkc
      181 ksnsegkvas yipqlarmnp dywgvsvcti dgqrfsigdt sipftlqscs kpltyaiald
      241 rlgqevvhqy vgqepsgrnf nelvldynkk phnpminaga ilvcsllksl ikpemtlaek
      301 fdftmnyfkr laggenlgfn navflserea adrnyalgfy mrehdcypdk snlreimdfy
      361 fqccsmeanc dtmavmaatl anggicpite ekvlkpdsvr dvlslmhscg mydysgqfaf
      421 kvgipaksgv sgsllvvipn vmgictwspp ldplgnscrg vqfceelvte fnfhstyydh
      481 rydnlkhatn kkdprrhkye tkglsivnll fsaasgdvta mrrhrlsgmd mtlsdydgrt
      541 alhlaasegh ldcveflleq cgvpdepkdr wgkrpideae sfghmqvvey lnnyalarkt
      601 eteneenevk eentdsrevv etagrtplp
//
LOCUS       XP_034186775             623 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034186775
VERSION     XP_034186775.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330884.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186775.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..623
                     /product="glutaminase isoform X5"
                     /calculated_mol_wt=70552
     Region          73..164
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          186..474
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          503..585
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          503..529
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(504..505,508..510,512..513,517,520,529,531,533,
                     537..538,541..543,545..546,550,553,563,565,567,571..572,
                     575..577,579..580,584)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          531..563
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..623
                     /gene="GLS"
                     /coded_by="XM_034330884.2:567..2438"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mkdksqsivv sdsemnneys denpemmpak fsedysirtk iactmwlekq gyktrrmlar
       61 flstrdqdqa tnaedvlfdm fkneetgllp vgkflaalrt tglrnddprl qeftdnlrke
      121 hlksgghegv shetqklsre qfrriispni vlisrafrhq fiipdwsgft khiedfywkc
      181 ksnsegkvas yipqlarmnp dywgvsvcti dgqrfsigdt sipftlqscs kpltyaiald
      241 rlgqevvhqy vgqepsgrnf nelvldynkk phnpminaga ilvcsllksl ikpemtlaek
      301 fdftmnyfkr laggenlgfn navflserea adrnyalgfy mrehdcypdk snlreimdfy
      361 fqccsmeanc dtmavmaatl anggicpite ekvlkpdsvr dvlslmhscg mydysgqfaf
      421 kvgipaksgv sgsllvvipn vmgictwspp ldplgnscrg vqfceelvte fnfhrydnlk
      481 hatnkkdprr hkyetkglsi vnllfsaasg dvtamrrhrl sgmdmtlsdy dgrtalhlaa
      541 seghldcvef lleqcgvpde pkdrwgkrpi deaesfghmq vveylnnyal arktetenee
      601 nevkeentds revvetagrt plp
//
LOCUS       XP_034186783             586 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034186783
VERSION     XP_034186783.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330892.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="glutaminase isoform X6"
                     /calculated_mol_wt=66386
     Region          30..121
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          143..431
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          466..548
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          466..492
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(467..468,471..473,475..476,480,483,492,494,496,
                     500..501,504..506,508..509,513,516,526,528,530,534..535,
                     538..540,542..543,547)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          494..526
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..586
                     /gene="GLS"
                     /coded_by="XM_034330892.2:532..2292"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mdsfdysfvd dfvdfrrcfc trdqdqatna edvlfdmfkn eetgllpvgk flaalrttgl
       61 rnddprlqef tdnlrkehlk sgghegvshe tqklsreqfr riispnivli srafrhqfii
      121 pdwsgftkhi edfywkcksn segkvasyip qlarmnpdyw gvsvctidgq rfsigdtsip
      181 ftlqscskpl tyaialdrlg qevvhqyvgq epsgrnfnel vldynkkphn pminagailv
      241 csllkslikp emtlaekfdf tmnyfkrlag genlgfnnav flsereaadr nyalgfymre
      301 hdcypdksnl reimdfyfqc csmeancdtm avmaatlang gicpiteekv lkpdsvrdvl
      361 slmhscgmyd ysgqfafkvg ipaksgvsgs llvvipnvmg ictwsppldp lgnscrgvqf
      421 ceelvtefnf hstyydhryd nlkhatnkkd prrhkyetkg lsivnllfsa asgdvtamrr
      481 hrlsgmdmtl sdydgrtalh laaseghldc veflleqcgv pdepkdrwgk rpideaesfg
      541 hmqvveylnn yalarktete neenevkeen tdsrevveta grtplp
//
LOCUS       XP_034186792             568 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034186792
VERSION     XP_034186792.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330901.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..568
                     /product="glutaminase isoform X7"
                     /calculated_mol_wt=64184
     Region          12..103
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          125..413
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          448..530
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          448..474
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(449..450,453..455,457..458,462,465,474,476,478,
                     482..483,486..488,490..491,495,498,508,510,512,516..517,
                     520..522,524..525,529)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          476..508
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..568
                     /gene="GLS"
                     /coded_by="XM_034330901.2:1283..2989"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mytrdqdqat naedvlfdmf kneetgllpv gkflaalrtt glrnddprlq eftdnlrkeh
       61 lksgghegvs hetqklsreq frriispniv lisrafrhqf iipdwsgftk hiedfywkck
      121 snsegkvasy ipqlarmnpd ywgvsvctid gqrfsigdts ipftlqscsk pltyaialdr
      181 lgqevvhqyv gqepsgrnfn elvldynkkp hnpminagai lvcsllksli kpemtlaekf
      241 dftmnyfkrl aggenlgfnn avflsereaa drnyalgfym rehdcypdks nlreimdfyf
      301 qccsmeancd tmavmaatla nggicpitee kvlkpdsvrd vlslmhscgm ydysgqfafk
      361 vgipaksgvs gsllvvipnv mgictwsppl dplgnscrgv qfceelvtef nfhstyydhr
      421 ydnlkhatnk kdprrhkyet kglsivnllf saasgdvtam rrhrlsgmdm tlsdydgrta
      481 lhlaaseghl dcveflleqc gvpdepkdrw gkrpideaes fghmqvveyl nnyalarkte
      541 teneenevke entdsrevve tagrtplp
//
LOCUS       XP_034186808             550 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034186808
VERSION     XP_034186808.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330917.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..550
                     /product="glutaminase isoform X8"
                     /calculated_mol_wt=62070
     Region          1..85
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          107..395
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          430..512
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          430..456
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(431..432,435..437,439..440,444,447,456,458,460,
                     464..465,468..470,472..473,477,480,490,492,494,498..499,
                     502..504,506..507,511)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          458..490
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..550
                     /gene="GLS"
                     /coded_by="XM_034330917.2:1302..2954"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mfkneetgll pvgkflaalr ttglrnddpr lqeftdnlrk ehlksggheg vshetqklsr
       61 eqfrriispn ivlisrafrh qfiipdwsgf tkhiedfywk cksnsegkva syipqlarmn
      121 pdywgvsvct idgqrfsigd tsipftlqsc skpltyaial drlgqevvhq yvgqepsgrn
      181 fnelvldynk kphnpminag ailvcsllks likpemtlae kfdftmnyfk rlaggenlgf
      241 nnavflsere aadrnyalgf ymrehdcypd ksnlreimdf yfqccsmean cdtmavmaat
      301 langgicpit eekvlkpdsv rdvlslmhsc gmydysgqfa fkvgipaksg vsgsllvvip
      361 nvmgictwsp pldplgnscr gvqfceelvt efnfhstyyd hrydnlkhat nkkdprrhky
      421 etkglsivnl lfsaasgdvt amrrhrlsgm dmtlsdydgr talhlaaseg hldcveflle
      481 qcgvpdepkd rwgkrpidea esfghmqvve ylnnyalark teteneenev keentdsrev
      541 vetagrtplp
//
LOCUS       XP_034186817             550 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminase isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034186817
VERSION     XP_034186817.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330926.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..550
                     /product="glutaminase isoform X8"
                     /calculated_mol_wt=62070
     Region          1..85
                     /region_name="EF-hand_14"
                     /note="EF-hand domain; pfam17959"
                     /db_xref="CDD:465587"
     Region          107..395
                     /region_name="Glutaminase"
                     /note="pfam04960"
                     /db_xref="CDD:461499"
     Region          430..512
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          430..456
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(431..432,435..437,439..440,444,447,456,458,460,
                     464..465,468..470,472..473,477,480,490,492,494,498..499,
                     502..504,506..507,511)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          458..490
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..550
                     /gene="GLS"
                     /coded_by="XM_034330926.2:279..1931"
                     /db_xref="GeneID:117607300"
ORIGIN      
        1 mfkneetgll pvgkflaalr ttglrnddpr lqeftdnlrk ehlksggheg vshetqklsr
       61 eqfrriispn ivlisrafrh qfiipdwsgf tkhiedfywk cksnsegkva syipqlarmn
      121 pdywgvsvct idgqrfsigd tsipftlqsc skpltyaial drlgqevvhq yvgqepsgrn
      181 fnelvldynk kphnpminag ailvcsllks likpemtlae kfdftmnyfk rlaggenlgf
      241 nnavflsere aadrnyalgf ymrehdcypd ksnlreimdf yfqccsmean cdtmavmaat
      301 langgicpit eekvlkpdsv rdvlslmhsc gmydysgqfa fkvgipaksg vsgsllvvip
      361 nvmgictwsp pldplgnscr gvqfceelvt efnfhstyyd hrydnlkhat nkkdprrhky
      421 etkglsivnl lfsaasgdvt amrrhrlsgm dmtlsdydgr talhlaaseg hldcveflle
      481 qcgvpdepkd rwgkrpidea esfghmqvve ylnnyalark teteneenev keentdsrev
      541 vetagrtplp
//
LOCUS       XP_034186832             429 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/potassium-transporting ATPase subunit beta-1-interacting
            protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186832
VERSION     XP_034186832.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330941.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..429
                     /product="sodium/potassium-transporting ATPase subunit
                     beta-1-interacting protein isoform X1"
                     /calculated_mol_wt=49120
     Region          1..175
                     /region_name="NKAIN"
                     /note="Na,K-Atpase Interacting protein; pfam05640"
                     /db_xref="CDD:461699"
     CDS             1..429
                     /gene="NKAIN"
                     /coded_by="XM_034330941.2:429..1718"
                     /db_xref="GeneID:117607343"
ORIGIN      
        1 mgicnrrhfl lticilqlit tverqvfdfl gfmwapilvn ffniifvilg ffgafqyrpk
       61 yiisycvwnt lwlgwnifmi cfylnvgild knsdilnlgt gsfswwhvng pgckaiydvt
      121 epelfrparp anvtdcvldy evveilhast qcilgfiaiv ggiclskvfl eeddslqakr
      181 kkkqprplys ieysqpeppa vhdesvspkp mtprrvkrrs visrdgtrrs srrssvrqsr
      241 rknpvnrimd qqeslyantt psnltnqnvn slsqgtlnyd rismtwdrdr nsnwqpeavn
      301 mavssptiwn qdssnnysnt snytnqnypn wdatsstrnl tdnwqanels pmqwqgqsnp
      361 tfqqtstqsl ngedldeiyn nrpasarssy snyhgvratp qvpnrtdivr qsqrqfvlsg
      421 ppayqdtvi
//
LOCUS       XP_076546370             397 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/potassium-transporting ATPase subunit beta-1-interacting
            protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546370
VERSION     XP_076546370.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690255.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="sodium/potassium-transporting ATPase subunit
                     beta-1-interacting protein isoform X2"
                     /calculated_mol_wt=45109
     Region          1..198
                     /region_name="NKAIN"
                     /note="Na,K-Atpase Interacting protein; pfam05640"
                     /db_xref="CDD:461699"
     CDS             1..397
                     /gene="NKAIN"
                     /coded_by="XM_076690255.1:417..1610"
                     /db_xref="GeneID:117607343"
ORIGIN      
        1 mgicnrrhfl lticilqlit tverqvfdfl gfmwapilvn ffniifvilg ffgafqyrpk
       61 yiisycvwnt lwlgwnifmi cfylnvgild knsdilnlgt gsfswwhvng pgckaiydvt
      121 epelfrparp anvtdcvldy evveilhast qcilgfiaiv ggiclskvfl eeddsfdfvg
      181 gddfglaght plhpmyvsys alpspahpqk nsaqnypagt asshqsvchd nnsfskntdk
      241 lfnssvrskn sfkndtirtn rsnqsnrdlk qvdypndysn pldhlqrpvs pydeydslds
      301 aaklryqksk myypqsssss ryspvaspri ksrpgakqsk asnkprvftd hvreqplrsf
      361 ysdprlanen qqntneqeiq nrakrdsrpl slyasnf
//
LOCUS       XP_076546371             394 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/potassium-transporting ATPase subunit beta-1-interacting
            protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546371
VERSION     XP_076546371.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690256.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..394
                     /product="sodium/potassium-transporting ATPase subunit
                     beta-1-interacting protein isoform X3"
                     /calculated_mol_wt=44719
     Region          1..198
                     /region_name="NKAIN"
                     /note="Na,K-Atpase Interacting protein; pfam05640"
                     /db_xref="CDD:461699"
     CDS             1..394
                     /gene="NKAIN"
                     /coded_by="XM_076690256.1:420..1604"
                     /db_xref="GeneID:117607343"
ORIGIN      
        1 mgicnrrhfl lticilqlit tverqvfdfl gfmwapilvn ffniifvilg ffgafqyrpk
       61 yiisycvwnt lwlgwnifmi cfylnvgild knsdilnlgt gsfswwhvng pgckaiydvt
      121 epelfrparp anvtdcvldy evveilhast qcilgfiaiv ggiclskvfl eeddsfdfvg
      181 gddfglaght plhpmyvsys alpspahpqk nsaqnypagt asshqsvchd nnsfskntdk
      241 lfnssvrskn sfkndtirtn rsnqsnrdlk qvdypndysn pldhlqrpvs pydeydslds
      301 aaklryqksk myypqsssss ryspvaspri ksrpgakqsk asnkprvftd hvreqplrsf
      361 ysdprlanen qqntneqeiq nrakrdsrpl sfas
//
LOCUS       XP_034181045            1062 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117604738 [Osmia
            lignaria lignaria].
ACCESSION   XP_034181045
VERSION     XP_034181045.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325154.2
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 1% of CDS bases
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1062
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117604738"
                     /calculated_mol_wt=117029
     Region          373..393
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          401..421
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(406,408,410,412..413,416..417,420,434,436,440..441,
                     444..445,448,462,464,466,468..469,472..473,476)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          422..>495
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          429..449
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          457..477
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          <616..737
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          <905..>1015
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          910..930
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          938..958
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(943,945,947,949..950,953..954,957,971,973,977..978,
                     981..982,985,999,1001,1003,1005..1006,1009..1010,1013)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          950..975
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          966..986
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          992..1014
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          994..1014
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1062
                     /gene="LOC117604738"
                     /coded_by="XM_034325154.2:2640..5828"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:117604738"
ORIGIN      
        1 mnftpfpgft tgslptgtvh qfatakfaqn lptggqvvgv lsggeggvhy lrpvdpngfa
       61 vaqggnnqqq qiitlpitvp gkdgtqqqqt vqiqvvnpsv sqsgnsgdqp kyhlapislg
      121 qfpqgaatvl tvaysqqgad gvqiqvqpqn tvattqtsdq ttqststavt ttsqqtiqqt
      181 vqlpgtpegl tvvaqipqdl ilregmeesk edvkegttpv alikrgnikn qgnpsgeefi
      241 tsmpaswqsl atpgstvady lsrlpastlp lslqhflkfs aetikreati essplgadgl
      301 dasgsvvsge qavqitvagg etaiipdvvd eqepgakpkr kkkykkkppk pkkpkpgqvs
      361 ivtaldgttl fccpqcnmay pekelleqhl ighkierrfi cdicgaglkr kehlerhklg
      421 hnpdrpfics vcmkgfkrke hlnlhfvihs gqktevctec gkafyrkdhl rkharshlak
      481 rikeeplnnv dasqnaqqqt dqqqqqaeql qqqaeqlqqq aeqlqqqadq lqqqssvsel
      541 qqiqiqvgqg sqaqihqiav teqstvvlpt aaetgamaml hhqqqqqqqq qqqqqqqqqq
      601 qqqqpqhxqq htapnqpgry frfnhqqarg rarrfamnfp pfgghfpgti psihqfatdg
      661 sggaggryan hfpnqppigv pvmgkyrpes ngvqsaqsqv mmnnkasypn snvhhypnvp
      721 ysqaqpvqqr psnspgmpek rsyhqqatet inqqdvcnll qdkdkskdkk deqqrngett
      781 tngnqqeysm hqhhqqhaht qtpatmpatw qslatpgstv adylshlpas tlplslhhfl
      841 kysaesikke semaqttsva vattttpnim mspnnkkkkk kkppkekkpr pkpgeirltt
      901 aldgstlycc pechmayper elleqhllgh tlerrfvcdi cgaglkrkdh ltrhkqshnp
      961 erpyvctvcl kafkrkeqlt lhfvihsgek rhvctecgkg fyrkdhlrkh trshiarrvk
     1021 aelsqnagtq qnqqqnnvsn mqttavtass vlsgghpgpl ls
//
LOCUS       XP_076546372             228 aa            linear   INV 03-SEP-2025
DEFINITION  clarin-2 [Osmia lignaria lignaria].
ACCESSION   XP_076546372
VERSION     XP_076546372.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690257.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="clarin-2"
                     /calculated_mol_wt=24939
     CDS             1..228
                     /gene="LOC143305693"
                     /coded_by="XM_076690257.1:1..687"
                     /db_xref="GeneID:143305693"
ORIGIN      
        1 mtsmfrrgti fatfflsllg gglvcaalvt qhwvearplr tlnpqvssgr vhfgllqgkk
       61 elnvgygwrt yhisvpqmik qdptvmswgl wistltttsa alvtaglaal lavlntatsp
      121 rskilsdpgv yfvnvltllm claststwla qyytklyfnv llkedidnmw tsegsaelgy
      181 sfwlvvcagv vhlisialvg wgsgrdkier letipaleek taaaimly
//
LOCUS       XP_076546373            1213 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: transmembrane channel-like protein [Osmia
            lignaria lignaria].
ACCESSION   XP_076546373
VERSION     XP_076546373.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690258.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 1% of CDS bases
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1213
                     /product="LOW QUALITY PROTEIN: transmembrane channel-like
                     protein"
                     /calculated_mol_wt=139408
     Region          843..958
                     /region_name="TMC"
                     /note="TMC domain; pfam07810"
                     /db_xref="CDD:462275"
     CDS             1..1213
                     /gene="LOC117611824"
                     /coded_by="XM_076690258.1:1..3642"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:117611824"
ORIGIN      
        1 mvplklspaf sfdtprgvtm ddvepsspqp svdrlsvtfa ivednnvqdn qkvasneknd
       61 vdmqrisiag lfnvnpxllf dqvfykldrn ncwtfgvsee eadeddysas acaimqrrss
      121 srrqsrrkrr psspfsveae atlrrrssiy tissgetais meesgsqeqi feklklhkev
      181 lggvkqqpwp lrrkiklvrq aksyvrrheg vlqerlaqtr stkdaiaris lfvtkkwqyc
      241 rremvnlqtw lipwefrike ieshfgsava syftflrwlf winlvmaail tafvaipeml
      301 tadplaager kimlreekik skhlltlwef egvlkyspff ygwytnqdsk sgyrlplayf
      361 vtnlvvyiys fvailrkmae nsrlskltek edeyvfswkl ftgwdfmign tetahnrmas
      421 ivlgfkeall eeaekekder nwkvitmrif vnisiisllg lsayavikvv arsaeeleqt
      481 swwrqneitv vmslitylfp lffeilglle syhprkqlrl qlarimvlnl lnlyslifal
      541 fgrinsmkqd lrnlqptikn csykpivcde enmtksrqla tlaslslimv agnatgmqsq
      601 neipeammpn lylnpildek sleeiynvgd yppldytydn ydgeeikine fnetsfsten
      661 ttqvfnltsd mfqqndafnm tttfvidnef fsnsteeind tsitsvpfie tstaynetse
      721 afyntddtsd dthdftttsf imdttsstte vntldklitv sdtstessii assyaerytd
      781 dttykittid sslssvnerv iplseenyil kcfeqicttt trdtvasskl ldlktrkklr
      841 rlcwettfgq elakltvmdl vlsilatlsi dflravfvrf mnsfwcwdle kqfpeygdfk
      901 iaenilhlvn nqgmiwmgmf fspgltvlnl lklgilmylr swavltcnvp hevifrasrs
      961 nnfyfalllt mlflcvlpvg yaivwvepsw hcgpfsgynk myhlatkqlt dslpgpiqrc
     1021 ldyiaspgiv iplivlmtli iyymvsltgs lreanndlki qlrherteer rklfrivkkr
     1081 eeltdtsflk wkkmlpalpr kpiksevskg epevvvtmen svevdveerk rsasevvelr
     1141 dikqdvvpqd haqnsvpenn snekgsisnr makflhdswe sqtsdhamip eirineaggk
     1201 siskennnvn hkk
//
LOCUS       XP_034183636             286 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183636
VERSION     XP_034183636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=31287
     Region          54..144
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          64..68
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          77..81
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          111..115
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          125..130
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..286
                     /gene="LOC117605925"
                     /coded_by="XM_034327745.2:9590..10450"
                     /db_xref="GeneID:117605925"
ORIGIN      
        1 mrtllilasi fleihlhftg sqaehqrsls sgnevvsske sedknedvie gpvrevlahs
       61 gstanlpcki tdpgagtitw vkhkgrrllt vgtsvhsidn rllvmhsstd wslqiravtl
      121 qdagiyecqi tshpvqrnfv hlkiteaysi ipgapdlhvk qgsslrlecq lmaaaesply
      181 vfwyrqghmi nydeepgvnv eltkggsilm vnktkpshgg nytcapsnak payvmvhvie
      241 eeekpaamhg gdrrnlspai lasnflvsll aaaswripsf tslypt
//
LOCUS       XP_034183656             286 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183656
VERSION     XP_034183656.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327765.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=31287
     Region          54..144
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          64..68
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          77..81
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          111..115
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          125..130
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..286
                     /gene="LOC117605925"
                     /coded_by="XM_034327765.2:9479..10339"
                     /db_xref="GeneID:117605925"
ORIGIN      
        1 mrtllilasi fleihlhftg sqaehqrsls sgnevvsske sedknedvie gpvrevlahs
       61 gstanlpcki tdpgagtitw vkhkgrrllt vgtsvhsidn rllvmhsstd wslqiravtl
      121 qdagiyecqi tshpvqrnfv hlkiteaysi ipgapdlhvk qgsslrlecq lmaaaesply
      181 vfwyrqghmi nydeepgvnv eltkggsilm vnktkpshgg nytcapsnak payvmvhvie
      241 eeekpaamhg gdrrnlspai lasnflvsll aaaswripsf tslypt
//
LOCUS       XP_034183646             286 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183646
VERSION     XP_034183646.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327755.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=31287
     Region          54..144
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          64..68
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          77..81
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          111..115
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          125..130
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..286
                     /gene="LOC117605925"
                     /coded_by="XM_034327755.2:9502..10362"
                     /db_xref="GeneID:117605925"
ORIGIN      
        1 mrtllilasi fleihlhftg sqaehqrsls sgnevvsske sedknedvie gpvrevlahs
       61 gstanlpcki tdpgagtitw vkhkgrrllt vgtsvhsidn rllvmhsstd wslqiravtl
      121 qdagiyecqi tshpvqrnfv hlkiteaysi ipgapdlhvk qgsslrlecq lmaaaesply
      181 vfwyrqghmi nydeepgvnv eltkggsilm vnktkpshgg nytcapsnak payvmvhvie
      241 eeekpaamhg gdrrnlspai lasnflvsll aaaswripsf tslypt
//
LOCUS       XP_034183664             286 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183664
VERSION     XP_034183664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=31287
     Region          54..144
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          64..68
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          77..81
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          111..115
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          125..130
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..286
                     /gene="LOC117605925"
                     /coded_by="XM_034327773.2:9391..10251"
                     /db_xref="GeneID:117605925"
ORIGIN      
        1 mrtllilasi fleihlhftg sqaehqrsls sgnevvsske sedknedvie gpvrevlahs
       61 gstanlpcki tdpgagtitw vkhkgrrllt vgtsvhsidn rllvmhsstd wslqiravtl
      121 qdagiyecqi tshpvqrnfv hlkiteaysi ipgapdlhvk qgsslrlecq lmaaaesply
      181 vfwyrqghmi nydeepgvnv eltkggsilm vnktkpshgg nytcapsnak payvmvhvie
      241 eeekpaamhg gdrrnlspai lasnflvsll aaaswripsf tslypt
//
LOCUS       XP_034183674             286 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183674
VERSION     XP_034183674.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327783.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=31287
     Region          54..144
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          64..68
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          77..81
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          111..115
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          125..130
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..286
                     /gene="LOC117605925"
                     /coded_by="XM_034327783.2:821..1681"
                     /db_xref="GeneID:117605925"
ORIGIN      
        1 mrtllilasi fleihlhftg sqaehqrsls sgnevvsske sedknedvie gpvrevlahs
       61 gstanlpcki tdpgagtitw vkhkgrrllt vgtsvhsidn rllvmhsstd wslqiravtl
      121 qdagiyecqi tshpvqrnfv hlkiteaysi ipgapdlhvk qgsslrlecq lmaaaesply
      181 vfwyrqghmi nydeepgvnv eltkggsilm vnktkpshgg nytcapsnak payvmvhvie
      241 eeekpaamhg gdrrnlspai lasnflvsll aaaswripsf tslypt
//
LOCUS       XP_034183684             286 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183684
VERSION     XP_034183684.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327793.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=31287
     Region          54..144
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          64..68
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          77..81
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          111..115
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          125..130
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..286
                     /gene="LOC117605925"
                     /coded_by="XM_034327793.2:611..1471"
                     /db_xref="GeneID:117605925"
ORIGIN      
        1 mrtllilasi fleihlhftg sqaehqrsls sgnevvsske sedknedvie gpvrevlahs
       61 gstanlpcki tdpgagtitw vkhkgrrllt vgtsvhsidn rllvmhsstd wslqiravtl
      121 qdagiyecqi tshpvqrnfv hlkiteaysi ipgapdlhvk qgsslrlecq lmaaaesply
      181 vfwyrqghmi nydeepgvnv eltkggsilm vnktkpshgg nytcapsnak payvmvhvie
      241 eeekpaamhg gdrrnlspai lasnflvsll aaaswripsf tslypt
//
LOCUS       XP_034183695             286 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183695
VERSION     XP_034183695.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327804.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=31287
     Region          54..144
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          64..68
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          77..81
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          111..115
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          125..130
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          155..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          181..184
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..286
                     /gene="LOC117605925"
                     /coded_by="XM_034327804.2:670..1530"
                     /db_xref="GeneID:117605925"
ORIGIN      
        1 mrtllilasi fleihlhftg sqaehqrsls sgnevvsske sedknedvie gpvrevlahs
       61 gstanlpcki tdpgagtitw vkhkgrrllt vgtsvhsidn rllvmhsstd wslqiravtl
      121 qdagiyecqi tshpvqrnfv hlkiteaysi ipgapdlhvk qgsslrlecq lmaaaesply
      181 vfwyrqghmi nydeepgvnv eltkggsilm vnktkpshgg nytcapsnak payvmvhvie
      241 eeekpaamhg gdrrnlspai lasnflvsll aaaswripsf tslypt
//
LOCUS       XP_034183708             276 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183708
VERSION     XP_034183708.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327817.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=30479
     Region          50..137
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          60..64
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..108
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..123
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          148..222
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          158..162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          172..177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..203
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          213..219
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..276
                     /gene="LOC117605946"
                     /coded_by="XM_034327817.2:555..1385"
                     /db_xref="GeneID:117605946"
ORIGIN      
        1 msalrlilll lassrylvhp tdagvrdekt sneggrnqnn vlpsvaasvk dvlaqtggna
       61 nlpcrftgpg ivtwirrkdr qlltvgrrth avdprfmvsn spdwnllikn vkrddaglye
      121 cqiqtepvqq rfvrlsitea ystipggpdl hvkqgsslrl ecqlmastet psfifwyreg
      181 rminyddepg vrveatkngs ilvvdkvkls hganytcsps naraayimih vieeeekpaa
      241 mhggdrrngt srptinvvli fltflgvqnf nrgcat
//
LOCUS       XP_034183727             276 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183727
VERSION     XP_034183727.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327836.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=30479
     Region          50..137
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          60..64
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..108
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..123
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          148..222
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          158..162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          172..177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..203
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          213..219
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..276
                     /gene="LOC117605946"
                     /coded_by="XM_034327836.2:187..1017"
                     /db_xref="GeneID:117605946"
ORIGIN      
        1 msalrlilll lassrylvhp tdagvrdekt sneggrnqnn vlpsvaasvk dvlaqtggna
       61 nlpcrftgpg ivtwirrkdr qlltvgrrth avdprfmvsn spdwnllikn vkrddaglye
      121 cqiqtepvqq rfvrlsitea ystipggpdl hvkqgsslrl ecqlmastet psfifwyreg
      181 rminyddepg vrveatkngs ilvvdkvkls hganytcsps naraayimih vieeeekpaa
      241 mhggdrrngt srptinvvli fltflgvqnf nrgcat
//
LOCUS       XP_034183718             276 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183718
VERSION     XP_034183718.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327827.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=30479
     Region          50..137
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          60..64
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..108
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..123
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          148..222
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          158..162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          172..177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..203
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          213..219
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..276
                     /gene="LOC117605946"
                     /coded_by="XM_034327827.2:880..1710"
                     /db_xref="GeneID:117605946"
ORIGIN      
        1 msalrlilll lassrylvhp tdagvrdekt sneggrnqnn vlpsvaasvk dvlaqtggna
       61 nlpcrftgpg ivtwirrkdr qlltvgrrth avdprfmvsn spdwnllikn vkrddaglye
      121 cqiqtepvqq rfvrlsitea ystipggpdl hvkqgsslrl ecqlmastet psfifwyreg
      181 rminyddepg vrveatkngs ilvvdkvkls hganytcsps naraayimih vieeeekpaa
      241 mhggdrrngt srptinvvli fltflgvqnf nrgcat
//
LOCUS       XP_034183736             276 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183736
VERSION     XP_034183736.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327845.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=30479
     Region          50..137
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          60..64
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..108
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..123
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          148..222
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          158..162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          172..177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..203
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          213..219
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..276
                     /gene="LOC117605946"
                     /coded_by="XM_034327845.2:1284..2114"
                     /db_xref="GeneID:117605946"
ORIGIN      
        1 msalrlilll lassrylvhp tdagvrdekt sneggrnqnn vlpsvaasvk dvlaqtggna
       61 nlpcrftgpg ivtwirrkdr qlltvgrrth avdprfmvsn spdwnllikn vkrddaglye
      121 cqiqtepvqq rfvrlsitea ystipggpdl hvkqgsslrl ecqlmastet psfifwyreg
      181 rminyddepg vrveatkngs ilvvdkvkls hganytcsps naraayimih vieeeekpaa
      241 mhggdrrngt srptinvvli fltflgvqnf nrgcat
//
LOCUS       XP_034183744             276 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034183744
VERSION     XP_034183744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="limbic system-associated membrane protein"
                     /calculated_mol_wt=30479
     Region          50..137
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          60..64
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..108
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..123
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          148..222
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          158..162
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          172..177
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..203
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          213..219
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..276
                     /gene="LOC117605946"
                     /coded_by="XM_034327853.2:806..1636"
                     /db_xref="GeneID:117605946"
ORIGIN      
        1 msalrlilll lassrylvhp tdagvrdekt sneggrnqnn vlpsvaasvk dvlaqtggna
       61 nlpcrftgpg ivtwirrkdr qlltvgrrth avdprfmvsn spdwnllikn vkrddaglye
      121 cqiqtepvqq rfvrlsitea ystipggpdl hvkqgsslrl ecqlmastet psfifwyreg
      181 rminyddepg vrveatkngs ilvvdkvkls hganytcsps naraayimih vieeeekpaa
      241 mhggdrrngt srptinvvli fltflgvqnf nrgcat
//
LOCUS       XP_076546374             145 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305694 [Osmia lignaria lignaria].
ACCESSION   XP_076546374
VERSION     XP_076546374.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690259.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..145
                     /product="uncharacterized protein LOC143305694"
                     /calculated_mol_wt=16451
     CDS             1..145
                     /gene="LOC143305694"
                     /coded_by="XM_076690259.1:1..438"
                     /db_xref="GeneID:143305694"
ORIGIN      
        1 mvnlnnssnn nsgtrnrdrs rsakretkrt eqgkvscsdr hdddddidee rndrenrrks
       61 qlpksskvti vrkdpwnvqs slrkyrrski tserkilred fskvsgivsi askedlarvg
      121 dieadvasns rtqsgtqvmq gkknn
//
LOCUS       XP_034183764             133 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605970 [Osmia lignaria lignaria].
ACCESSION   XP_034183764
VERSION     XP_034183764.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327873.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..133
                     /product="uncharacterized protein LOC117605970"
                     /calculated_mol_wt=14507
     Region          46..>120
                     /region_name="DUF4764"
                     /note="Domain of unknown function (DUF4764); pfam15961"
                     /db_xref="CDD:464955"
     CDS             1..133
                     /gene="LOC117605970"
                     /coded_by="XM_034327873.2:1175..1576"
                     /db_xref="GeneID:117605970"
ORIGIN      
        1 mafmmpvvkn ewdiyktnrs rrssecsnpq acrsrkvsec sksegpslst spgsdfltsp
       61 ahrsvpltsr hfsrtssras qsslqspsks tgssppktgs ssslnkfhnr lvdklkkslk
      121 kaednaedqr nls
//
LOCUS       XP_034184142             851 aa            linear   INV 03-SEP-2025
DEFINITION  neuroligin 5 [Osmia lignaria lignaria].
ACCESSION   XP_034184142
VERSION     XP_034184142.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328251.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184142.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..851
                     /product="neuroligin 5"
                     /calculated_mol_wt=94900
     Region          50..605
                     /region_name="COesterase"
                     /note="Carboxylesterase family; pfam00135"
                     /db_xref="CDD:395084"
     Site            order(182..184,266..268,271,412,416..417,457,503,506)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(267,390,502)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..851
                     /gene="NLG-5"
                     /coded_by="XM_034328251.2:1838..4393"
                     /db_xref="GeneID:117606144"
ORIGIN      
        1 mllpcqpaak krplatrslr kstsylvliv vvllligree sraatqirya srivetksgq
       61 irgilqelns rhldpvevfr gipyaappvg dlrfrppisp ipwdgiklad sfgavcpqhf
      121 pdirndtval lqmpldryqq lkrlymfltn qsedclflnl yipgsgsrgl eapyavmvyv
      181 hgesfewgtg niydgsvlas aghvivitln yrlgilgflr trpypdrtpg sggnlalkdi
      241 amglrwvren iaafggdptk itlighdtga alvnllllap ygkglfhrvv lssgsalspw
      301 asvhdpndlr lkvaeqigcs tendediadc lrgtplrqlm gvelpeirfv prigpglpvd
      361 qnnpdpgldm erisdtfikv plilgvsste snldfnandi qygfeedhrn rvlrtfirna
      421 yvyhlneifs avrneytdwd kpvlhpiiir dstmealsdg htvaplmria fyharrgakt
      481 yfyhfshqnk nsgymerlgs irgedipyif glplvagglf fprnysrqdq gvaeavltff
      541 tnfaktgnpn ephkiesvdy gtpkektryr gltweqyetg tqqyltiamk pkmkshyrgh
      601 kmavwlnlip qlhrpgdddv smrhhhfrer gdhfyagpvr dewytplplv gttkssasst
      661 tacststged svaeditpil ddseddaell qrltsrhyys tttalaitvg vgcillvlnm
      721 lifagiyyqr drdkkraamd ctpngqeslp mttrssikap dsprstqepp psyttlarsp
      781 siqehqqipq nqsadadeqs iceqkashat sntihkdpip pkpptrttss lsttgstnti
      841 kkrvqiqeis v
//
LOCUS       XP_076546375             851 aa            linear   INV 03-SEP-2025
DEFINITION  neuroligin 5 [Osmia lignaria lignaria].
ACCESSION   XP_076546375
VERSION     XP_076546375.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690260.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..851
                     /product="neuroligin 5"
                     /calculated_mol_wt=94900
     Region          50..605
                     /region_name="COesterase"
                     /note="Carboxylesterase family; pfam00135"
                     /db_xref="CDD:395084"
     Site            order(182..184,266..268,271,412,416..417,457,503,506)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(267,390,502)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..851
                     /gene="NLG-5"
                     /coded_by="XM_076690260.1:1675..4230"
                     /db_xref="GeneID:117606144"
ORIGIN      
        1 mllpcqpaak krplatrslr kstsylvliv vvllligree sraatqirya srivetksgq
       61 irgilqelns rhldpvevfr gipyaappvg dlrfrppisp ipwdgiklad sfgavcpqhf
      121 pdirndtval lqmpldryqq lkrlymfltn qsedclflnl yipgsgsrgl eapyavmvyv
      181 hgesfewgtg niydgsvlas aghvivitln yrlgilgflr trpypdrtpg sggnlalkdi
      241 amglrwvren iaafggdptk itlighdtga alvnllllap ygkglfhrvv lssgsalspw
      301 asvhdpndlr lkvaeqigcs tendediadc lrgtplrqlm gvelpeirfv prigpglpvd
      361 qnnpdpgldm erisdtfikv plilgvsste snldfnandi qygfeedhrn rvlrtfirna
      421 yvyhlneifs avrneytdwd kpvlhpiiir dstmealsdg htvaplmria fyharrgakt
      481 yfyhfshqnk nsgymerlgs irgedipyif glplvagglf fprnysrqdq gvaeavltff
      541 tnfaktgnpn ephkiesvdy gtpkektryr gltweqyetg tqqyltiamk pkmkshyrgh
      601 kmavwlnlip qlhrpgdddv smrhhhfrer gdhfyagpvr dewytplplv gttkssasst
      661 tacststged svaeditpil ddseddaell qrltsrhyys tttalaitvg vgcillvlnm
      721 lifagiyyqr drdkkraamd ctpngqeslp mttrssikap dsprstqepp psyttlarsp
      781 siqehqqipq nqsadadeqs iceqkashat sntihkdpip pkpptrttss lsttgstnti
      841 kkrvqiqeis v
//
LOCUS       XP_076546376             851 aa            linear   INV 03-SEP-2025
DEFINITION  neuroligin 5 [Osmia lignaria lignaria].
ACCESSION   XP_076546376
VERSION     XP_076546376.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690261.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..851
                     /product="neuroligin 5"
                     /calculated_mol_wt=94900
     Region          50..605
                     /region_name="COesterase"
                     /note="Carboxylesterase family; pfam00135"
                     /db_xref="CDD:395084"
     Site            order(182..184,266..268,271,412,416..417,457,503,506)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(267,390,502)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..851
                     /gene="NLG-5"
                     /coded_by="XM_076690261.1:547..3102"
                     /db_xref="GeneID:117606144"
ORIGIN      
        1 mllpcqpaak krplatrslr kstsylvliv vvllligree sraatqirya srivetksgq
       61 irgilqelns rhldpvevfr gipyaappvg dlrfrppisp ipwdgiklad sfgavcpqhf
      121 pdirndtval lqmpldryqq lkrlymfltn qsedclflnl yipgsgsrgl eapyavmvyv
      181 hgesfewgtg niydgsvlas aghvivitln yrlgilgflr trpypdrtpg sggnlalkdi
      241 amglrwvren iaafggdptk itlighdtga alvnllllap ygkglfhrvv lssgsalspw
      301 asvhdpndlr lkvaeqigcs tendediadc lrgtplrqlm gvelpeirfv prigpglpvd
      361 qnnpdpgldm erisdtfikv plilgvsste snldfnandi qygfeedhrn rvlrtfirna
      421 yvyhlneifs avrneytdwd kpvlhpiiir dstmealsdg htvaplmria fyharrgakt
      481 yfyhfshqnk nsgymerlgs irgedipyif glplvagglf fprnysrqdq gvaeavltff
      541 tnfaktgnpn ephkiesvdy gtpkektryr gltweqyetg tqqyltiamk pkmkshyrgh
      601 kmavwlnlip qlhrpgdddv smrhhhfrer gdhfyagpvr dewytplplv gttkssasst
      661 tacststged svaeditpil ddseddaell qrltsrhyys tttalaitvg vgcillvlnm
      721 lifagiyyqr drdkkraamd ctpngqeslp mttrssikap dsprstqepp psyttlarsp
      781 siqehqqipq nqsadadeqs iceqkashat sntihkdpip pkpptrttss lsttgstnti
      841 kkrvqiqeis v
//
LOCUS       XP_034184170             422 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, mu subunit [Osmia lignaria lignaria].
ACCESSION   XP_034184170
VERSION     XP_034184170.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328279.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..422
                     /product="adaptor protein complex 1, mu subunit"
                     /calculated_mol_wt=48483
     Region          4..142
                     /region_name="AP1_Mu_N"
                     /note="AP-1 complex subunit mu N-terminal domain; cd14835"
                     /db_xref="CDD:341439"
     Site            order(19,22,45..48,56,58,60,72..78,81,85,111..112,
                     115..116,118..120,122..125,134,136..137)
                     /site_type="other"
                     /note="AP-1 beta interface [polypeptide binding]"
                     /db_xref="CDD:341439"
     Region          152..421
                     /region_name="AP-1_Mu1A_Cterm"
                     /note="C-terminal domain of medium Mu1A subunit in
                     ubiquitously expressed clathrin-associated adaptor protein
                     (AP) complex AP-1; cd09258"
                     /db_xref="CDD:271166"
     Site            order(165,185,187,189..190,200,210,233,236,260,267,269,
                     271,283,295,297,303,308,327,332,335..336,345,351..352,354,
                     378..380,383,392..395,404..408,413,415..416)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:271166"
     Site            order(171..173,200,307,380,382,406..409,411)
                     /site_type="signal peptide"
                     /note="signal peptide binding site [polypeptide binding]"
                     /db_xref="CDD:271166"
     CDS             1..422
                     /gene="AP-1mu"
                     /coded_by="XM_034328279.2:169..1437"
                     /db_xref="GeneID:117606167"
ORIGIN      
        1 mstsaiyild vkgkvlisrn yrgdietgvi ekfmphvmer eeegnltpii qttectyayi
       61 kynnlyivst tkknanislv fvflhklvqv mqeyfkelee esirdnfvvi yelldelidf
      121 gypqttdski lqeyitqegh kleiqpripm avtnavswrs egikyrknev fldviesvnl
      181 lanangnvls seivgaikmr vylsgmpelr lglndkvlfe stgrgksksv eledvkfhqc
      241 vrlsrfendr tisfippdge felmsyrlnt hvkpliwies vierhahsrv eymikarsqf
      301 krrstannve ivipvpndad spkfkttigs vkyspeqsai twfiksfpgg keylmrahfg
      361 lpsvigedve gkppiqvkfe ipyfttsgiq vrylkiieks gyqalpwvry itqngdyqlr
      421 tn
//
LOCUS       XP_034184181             407 aa            linear   INV 03-SEP-2025
DEFINITION  la autoantigen-like [Osmia lignaria lignaria].
ACCESSION   XP_034184181
VERSION     XP_034184181.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328290.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..407
                     /product="la autoantigen-like"
                     /calculated_mol_wt=46792
     Region          45..122
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(53,56..57,62,65..66,68,88..90)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153396"
     Region          143..219
                     /region_name="RRM1_La"
                     /note="RNA recognition motif 1 in La autoantigen (La or
                     LARP3) and similar proteins; cd12291"
                     /db_xref="CDD:409733"
     Site            order(156,171..173,191,193)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:409733"
     Region          274..349
                     /region_name="RRM2_La"
                     /note="RNA recognition motif 2 in La autoantigen (La or
                     LARP3) and similar proteins; cd12541"
                     /db_xref="CDD:409957"
     CDS             1..407
                     /gene="La"
                     /coded_by="XM_034328290.2:166..1389"
                     /db_xref="GeneID:117606172"
ORIGIN      
        1 mengqeetkv ekvqndvaan eskcekpvsn aeekaeqkve epskellqki ksqiefyfgd
       61 vnmqrdkfli eqtklddgwi pmnimlnfkm laslskdidv ilkavelsdi meisedkkki
      121 rrspehplpv yneeyrkaqe aktvyikgfp lsdtnieklk tffndfepfe nivmrkyldk
      181 dkklqfkgsi fvqfktleda kafmardsik fgetelikkw sadysvekak eredkkqkka
      241 egkskksena kdkedeqsge eeeengkeva lpkgcvihfs gvprsctred ikerlgelda
      301 siafvdfkig dekgwvrlqg enaakavvdk mkdskvlirg khvtcrileg eeeekylika
      361 qgemsnlrqk ypkgkkskrg ryaqrgqrkr rnsdsadstp akkttve
//
LOCUS       XP_034184459             878 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin carboxyl-terminal hydrolase 35 [Osmia lignaria lignaria].
ACCESSION   XP_034184459
VERSION     XP_034184459.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328568.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184459.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..878
                     /product="ubiquitin carboxyl-terminal hydrolase 35"
                     /calculated_mol_wt=101234
     Region          405..794
                     /region_name="Peptidase_C19H"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02664"
                     /db_xref="CDD:239129"
     Site            order(408,413,751,768)
                     /site_type="active"
                     /db_xref="CDD:239129"
     CDS             1..878
                     /gene="LOC117606273"
                     /coded_by="XM_034328568.2:131..2767"
                     /db_xref="GeneID:117606273"
ORIGIN      
        1 meteqilkyd ikqclnlivv gddvevnegw trlkkleses vyeqlsnyes ilrevlnsei
       61 knvhevprim vwfakylpqe pltvhlisnd iatmlrntei sdmshltmil qvlldhsiyl
      121 pdfinhaklc eaiiislsnf smpdeskril efndnatkve yflkmvqtrs kniennnlif
      181 tclqtlyrii sdtkrkqdpg pglaavlqvv epsiipqavl wilseshcds qlaqalkvlc
      241 swlpkwrgdr lsiwimefil glekqhkysi lmevtkasly vmfcalsvpv irqnastiif
      301 yvlkrqghps lfrnivrntq milsyltked sesskeciqn lvdimktlml rfpglsvfnn
      361 lenslpvppr mhivkelwte hvwvdeieev epvieplkph vekvglsnlg ntcymnsvlq
      421 allmtkrfcy evllykpmsk addqvvlkkl qnlftlllys kritlaptei llasrpayfl
      481 pgqqqdssef lchlldllhe qeksapince gnnsnlkykd dkeindissv neegvsikrw
      541 tteedltgsi alerktqsla dfthgedlaq vqqlsdshsd stdsgiqsvg gedtstqvpl
      601 vhrvlggkck ityqcaqcdt sshntdkfrd lqlcfpeeiq enqevsvqdl inyyltpekl
      661 tgenkyrcdk cmklcdaqri ikilhaptyl ililkhfryd fdtrprtklr hkvmynehiq
      721 lpvstplctt tetyqlyaav vhsgysmdyg hyftyardsk qnwykfndsy vsqttfndfk
      781 elkppdtpyi lfyekivpfe gvydedkpel stlskhlqel vendttayie elrhqaeksq
      841 rgrhnsmllr knensddenp ppsscrgavn mpasrfly
//
LOCUS       XP_034184220             624 aa            linear   INV 03-SEP-2025
DEFINITION  iroquois-class homeodomain protein mirror isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184220
VERSION     XP_034184220.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328329.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="iroquois-class homeodomain protein mirror
                     isoform X1"
                     /calculated_mol_wt=64397
     Site            order(225..227,229,249,255,268,270..271,274..275,277..279,
                     281..282)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Site            order(225,228,271,274..275,278)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          241..279
                     /region_name="Homeobox_KN"
                     /note="Homeobox KN domain; pfam05920"
                     /db_xref="CDD:428673"
     Region          426..443
                     /region_name="IRO"
                     /note="Motif in Iroquois-class homeodomain proteins
                     (only). Unknown function; smart00548"
                     /db_xref="CDD:214716"
     CDS             1..624
                     /gene="mirr"
                     /coded_by="XM_034328329.2:3451..5325"
                     /db_xref="GeneID:117606193"
ORIGIN      
        1 msqfsfrgsp nlqcpvtass sltsssaspv sgailsaags slvsvaattt nhplgvaagl
       61 snarmavtsa vvrppgsptt svspsqghpp pttggptptg rccdtgrpif tdpltgqtvc
      121 scqydllggy qrlgglptaa lsmysapyaa aaaaaasegm aayfpslgae qapfytptaa
      181 gldlkenlga gaaaawpyps vyhpydaafa sypfngygmd lngarrknat rettstlkaw
      241 lnehkknpyp tkgekimlai itkmtltqvs twfanarrrl kkenkmtwep rnrvedednn
      301 nedddsgrks vdekdrldsk dsgtgssedg erpahrmdll hstsggstgv qgrtesewse
      361 sradsgpdsp eclydqrepr hplqlqhpay lasahgrllr hpspestpps shhlppstta
      421 sstgsgvttk priwsladma skdcdqpspn pttmtglssp ysgsgggggg vaggggklvs
      481 plasrlpphh plhpamhsgt qfvrphpdfy rnlygashlg sgdmsllety srtlgglsgv
      541 mppstapsil tstssavsas gnvkpfting gsgapggpav llttatsgls psssstassg
      601 gsdqsphpsg slpptqelks pgrv
//
LOCUS       XP_034184232             560 aa            linear   INV 03-SEP-2025
DEFINITION  iroquois-class homeodomain protein mirror isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184232
VERSION     XP_034184232.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328341.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..560
                     /product="iroquois-class homeodomain protein mirror
                     isoform X2"
                     /calculated_mol_wt=58249
     Site            order(161..163,165,185,191,204,206..207,210..211,213..215,
                     217..218)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238039"
     Site            order(161,164,207,210..211,214)
                     /site_type="other"
                     /note="specific DNA base contacts [nucleotide binding]"
                     /db_xref="CDD:238039"
     Region          177..215
                     /region_name="Homeobox_KN"
                     /note="Homeobox KN domain; pfam05920"
                     /db_xref="CDD:428673"
     Region          362..379
                     /region_name="IRO"
                     /note="Motif in Iroquois-class homeodomain proteins
                     (only). Unknown function; smart00548"
                     /db_xref="CDD:214716"
     CDS             1..560
                     /gene="mirr"
                     /coded_by="XM_034328341.2:3680..5362"
                     /db_xref="GeneID:117606193"
ORIGIN      
        1 mavtsavvrp pgspttsvsp sqghpppttg gptptgrccd tgrpiftdpl tgqtvcscqy
       61 dllggyqrlg glptaalsmy sapyaaaaaa aasegmaayf pslgaeqapf ytptaagldl
      121 kenlgagaaa awpypsvyhp ydaafasypf ngygmdlnga rrknatrett stlkawlneh
      181 kknpyptkge kimlaiitkm tltqvstwfa narrrlkken kmtweprnrv edednnnedd
      241 dsgrksvdek drldskdsgt gssedgerpa hrmdllhsts ggstgvqgrt esewsesrad
      301 sgpdspecly dqreprhplq lqhpaylasa hgrllrhpsp estppsshhl ppsttasstg
      361 sgvttkpriw sladmaskdc dqpspnpttm tglsspysgs ggggggvagg ggklvsplas
      421 rlpphhplhp amhsgtqfvr phpdfyrnly gashlgsgdm slletysrtl gglsgvmpps
      481 tapsiltsts savsasgnvk pftinggsga pggpavlltt atsglspsss stassggsdq
      541 sphpsgslpp tqelkspgrv
//
LOCUS       XP_034184246             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184246
VERSION     XP_034184246.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184246.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328355.2:164..1588"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184255             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184255
VERSION     XP_034184255.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328364.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184255.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328364.2:679..2103"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184312             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184312
VERSION     XP_034184312.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328421.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184312.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328421.2:142..1566"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184262             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184262
VERSION     XP_034184262.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184262.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328371.2:746..2170"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184282             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184282
VERSION     XP_034184282.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328391.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184282.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328391.2:759..2183"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184292             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184292
VERSION     XP_034184292.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328401.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184292.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328401.2:313..1737"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184303             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184303
VERSION     XP_034184303.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328412.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184303.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328412.2:219..1643"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184322             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184322
VERSION     XP_034184322.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328431.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184322.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328431.2:327..1751"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184331             474 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184331
VERSION     XP_034184331.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328440.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184331.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="N-acetylgalactosamine kinase isoform X1"
                     /calculated_mol_wt=52675
     Region          24..473
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..474
                     /gene="Galk"
                     /coded_by="XM_034328440.2:232..1656"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkq
      181 plskrelati saraeryvgt mgggmdqaia flgkpgsaml iefdpvrgtd vtlpetavfv
      241 iahsqachnk astgdfnlrv vechlaalmi akkrsipler vrrlidvqeq lgmnleemvs
      301 vvttdlheep ytmdevsknl dttndklrkl lggfkdsqtf klkqralhvy qeaarvrefq
      361 rvsadssmme eeklqrlgsl mcksheslqk lyecshpsvd alvekamsyg algarltgag
      421 wggcivaiit kdkvsqfldk lktefvqcgi krgfelndlv fptepnqgav iytt
//
LOCUS       XP_034184341             385 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosamine kinase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034184341
VERSION     XP_034184341.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328450.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034184341.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..385
                     /product="N-acetylgalactosamine kinase isoform X2"
                     /calculated_mol_wt=43236
     Region          24..384
                     /region_name="PLN02521"
                     /note="galactokinase"
                     /db_xref="CDD:215285"
     CDS             1..385
                     /gene="Galk"
                     /coded_by="XM_034328450.2:164..1321"
                     /db_xref="GeneID:117606196"
ORIGIN      
        1 mlenangtse lepkervpii qpdektlekl diltdlfsdh yrrkpsfivr vpgrvnlige
       61 hvdycgyavc pmaieqdiii avapypdeih ivnmdfkykp fgcnfeelnf tvkepmgfvp
      121 mwykyflcgv kgalevipke ckpmgfvaav wgnippnsgl ssssalvsaa llltmhlnkm
      181 iakkrsiple rvrrlidvqe qlgmnleemv svvttdlhee pytmdevskn ldttndklrk
      241 llggfkdsqt fklkqralhv yqeaarvref qrvsadssmm eeeklqrlgs lmcksheslq
      301 klyecshpsv dalvekamsy galgarltga gwggcivaii tkdkvsqfld klktefvqcg
      361 ikrgfelndl vfptepnqga viytt
//
LOCUS       XP_076546377             445 aa            linear   INV 03-SEP-2025
DEFINITION  facilitated trehalose transporter Tret1 [Osmia lignaria lignaria].
ACCESSION   XP_076546377
VERSION     XP_076546377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690262.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="facilitated trehalose transporter Tret1"
                     /calculated_mol_wt=48658
     Region          5..437
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(18..19,22..23,26,61,111..112,114..116,119,139,
                     142..143,146,249,252..253,256..258,261,288,292,353..354,
                     358,362,378,381..382,385..386,389)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..445
                     /gene="LOC117606241"
                     /coded_by="XM_076690262.1:381..1718"
                     /db_xref="GeneID:117606241"
ORIGIN      
        1 mkklylatia snlgmlsygl cfgwaspslp vllqddspir ltaqqatwvt sfhtiggtig
       61 sllcyfilnv igrkwslllt aipavigwmm ialatsawel magrfsygls tgygyisatv
      121 ymgeispani rgiltstlti asklgvfiew tigpflslrn lalvsslapi ififsvlwvp
      181 espyqlmrse rhhqaieslm qlrgstdvsi eadaieksvk idlandtglw elfrvsgnrk
      241 alfivlglvi iqqwsgsqai msyaelifna tgdqlqgkyv tmilgaiqvv ctifgttvvd
      301 rfgrrpllmi safgtsvstf lvglffflqn netdvgmitw lpatgsilyv imyafglaal
      361 pftvmsevfp tnvkalgtgi gmfvcngsav ivtsiyqsiv eqygihaafw lfstislsgv
      421 vfvfyympet kgktlqevqe qlhgr
//
LOCUS       XP_076546378             330 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606226 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546378
VERSION     XP_076546378.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690263.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="uncharacterized protein LOC117606226 isoform X1"
                     /calculated_mol_wt=36383
     Region          75..181
                     /region_name="DUF4728"
                     /note="Domain of unknown function (DUF4728); pfam15860"
                     /db_xref="CDD:374176"
     CDS             1..330
                     /gene="LOC117606226"
                     /coded_by="XM_076690263.1:218..1210"
                     /db_xref="GeneID:117606226"
ORIGIN      
        1 maimqscccw rsvrrgsfac aiysgiyftv lalttgkvlq gesqylrgnr slpestsfle
       61 pdtispttvr fnvtllicsc cgvicsilll yglckdqrvf lipwiivvtt icfvdighsl
      121 ylfiaassfd ptkvmlytln ffllclnvvg rggdnlsgyi ptypllqiys llcvisqyqe
      181 ytngrgtaad dyeyrvsilr vsqreqdrdk grkvpavrya thpltttats clssrraatn
      241 netkvtatpt qsptgqntvs sekspvagks srkhvqfpdt asdsqkgkse ssitmlvank
      301 eteneyaips dptmidpsiq sqkmtevryt
//
LOCUS       XP_076546379             312 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606226 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546379
VERSION     XP_076546379.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690264.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="uncharacterized protein LOC117606226 isoform X2"
                     /calculated_mol_wt=34230
     Region          75..181
                     /region_name="DUF4728"
                     /note="Domain of unknown function (DUF4728); pfam15860"
                     /db_xref="CDD:374176"
     CDS             1..312
                     /gene="LOC117606226"
                     /coded_by="XM_076690264.1:219..1157"
                     /db_xref="GeneID:117606226"
ORIGIN      
        1 maimqscccw rsvrrgsfac aiysgiyftv lalttgkvlq gesqylrgnr slpestsfle
       61 pdtispttvr fnvtllicsc cgvicsilll yglckdqrvf lipwiivvtt icfvdighsl
      121 ylfiaassfd ptkvmlytln ffllclnvvg rggdnlsgyi ptypllqiys llcvisqyqe
      181 ytngrgtaad dyeyrvpavr yathplttta tsclssrraa tnnetkvtat ptqsptgqnt
      241 vssekspvag kssrkhvqfp dtasdsqkgk sessitmlva nketeneyai psdptmidps
      301 iqsqkmtevr yt
//
LOCUS       XP_034184354             310 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606226 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184354
VERSION     XP_034184354.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="uncharacterized protein LOC117606226 isoform X3"
                     /calculated_mol_wt=34281
     Region          75..161
                     /region_name="DUF4728"
                     /note="Domain of unknown function (DUF4728); pfam15860"
                     /db_xref="CDD:374176"
     CDS             1..310
                     /gene="LOC117606226"
                     /coded_by="XM_034328463.2:214..1146"
                     /db_xref="GeneID:117606226"
ORIGIN      
        1 maimqscccw rsvrrgsfac aiysgiyftv lalttgkvlq gesqylrgnr slpestsfle
       61 pdtispttvr fnvtllicsc cgvicsilll yglckdqrvf lipwiivvtt icfvdighsl
      121 ylfiaassfd ptkvmlytln ffllclniys llcvisqyqe ytngrgtaad dyeyrvsilr
      181 vsqreqdrdk grkvpavrya thpltttats clssrraatn netkvtatpt qsptgqntvs
      241 sekspvagks srkhvqfpdt asdsqkgkse ssitmlvank eteneyaips dptmidpsiq
      301 sqkmtevryt
//
LOCUS       XP_076546380             299 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606226 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546380
VERSION     XP_076546380.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690265.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..299
                     /product="uncharacterized protein LOC117606226 isoform X4"
                     /calculated_mol_wt=32837
     CDS             1..299
                     /gene="LOC117606226"
                     /coded_by="XM_076690265.1:218..1117"
                     /db_xref="GeneID:117606226"
ORIGIN      
        1 maimqscccw rsvrrgsfac aiysgiyftv lalttgkvlq gesqylrgnr slpestsfle
       61 pdtispttvr fnvtllicsc cgvicsilll yglckssfdp tkvmlytlnf fllclnvvgr
      121 ggdnlsgyip typllqiysl lcvisqyqey tngrgtaadd yeyrvsilrv sqreqdrdkg
      181 rkvpavryat hpltttatsc lssrraatnn etkvtatptq sptgqntvss ekspvagkss
      241 rkhvqfpdta sdsqkgkses sitmlvanke teneyaipsd ptmidpsiqs qkmtevryt
//
LOCUS       XP_034184364             292 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606226 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184364
VERSION     XP_034184364.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328473.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="uncharacterized protein LOC117606226 isoform X5"
                     /calculated_mol_wt=32129
     Region          75..161
                     /region_name="DUF4728"
                     /note="Domain of unknown function (DUF4728); pfam15860"
                     /db_xref="CDD:374176"
     CDS             1..292
                     /gene="LOC117606226"
                     /coded_by="XM_034328473.2:215..1093"
                     /db_xref="GeneID:117606226"
ORIGIN      
        1 maimqscccw rsvrrgsfac aiysgiyftv lalttgkvlq gesqylrgnr slpestsfle
       61 pdtispttvr fnvtllicsc cgvicsilll yglckdqrvf lipwiivvtt icfvdighsl
      121 ylfiaassfd ptkvmlytln ffllclniys llcvisqyqe ytngrgtaad dyeyrvpavr
      181 yathplttta tsclssrraa tnnetkvtat ptqsptgqnt vssekspvag kssrkhvqfp
      241 dtasdsqkgk sessitmlva nketeneyai psdptmidps iqsqkmtevr yt
//
LOCUS       XP_034184373             279 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606226 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184373
VERSION     XP_034184373.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328482.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..279
                     /product="uncharacterized protein LOC117606226 isoform X6"
                     /calculated_mol_wt=30735
     Region          <76..130
                     /region_name="DUF4728"
                     /note="Domain of unknown function (DUF4728); pfam15860"
                     /db_xref="CDD:374176"
     CDS             1..279
                     /gene="LOC117606226"
                     /coded_by="XM_034328482.2:215..1054"
                     /db_xref="GeneID:117606226"
ORIGIN      
        1 maimqscccw rsvrrgsfac aiysgiyftv lalttgkvlq gesqylrgnr slpestsfle
       61 pdtispttvr fnvtllicsc cgvicsilll yglckssfdp tkvmlytlnf fllclniysl
      121 lcvisqyqey tngrgtaadd yeyrvsilrv sqreqdrdkg rkvpavryat hpltttatsc
      181 lssrraatnn etkvtatptq sptgqntvss ekspvagkss rkhvqfpdta sdsqkgkses
      241 sitmlvanke teneyaipsd ptmidpsiqs qkmtevryt
//
LOCUS       XP_034184383             243 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606226 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184383
VERSION     XP_034184383.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328492.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="uncharacterized protein LOC117606226 isoform X7"
                     /calculated_mol_wt=26405
     Region          75..>129
                     /region_name="DUF4728"
                     /note="Domain of unknown function (DUF4728); pfam15860"
                     /db_xref="CDD:374176"
     CDS             1..243
                     /gene="LOC117606226"
                     /coded_by="XM_034328492.2:215..946"
                     /db_xref="GeneID:117606226"
ORIGIN      
        1 maimqscccw rsvrrgsfac aiysgiyftv lalttgkvlq gesqylrgnr slpestsfle
       61 pdtispttvr fnvtllicsc cgvicsilll yglckdqrvf lipwiivvtt icfvdighsl
      121 ylfiaavpav ryathplttt atsclssrra atnnetkvta tptqsptgqn tvssekspva
      181 gkssrkhvqf pdtasdsqkg ksessitmlv anketeneya ipsdptmidp siqsqkmtev
      241 ryt
//
LOCUS       XP_076546381             105 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546381
VERSION     XP_076546381.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690266.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="uncharacterized protein LOC143305696 isoform X1"
                     /calculated_mol_wt=11998
     CDS             1..105
                     /gene="LOC143305696"
                     /coded_by="XM_076690266.1:244..561"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanvg sevetqeyre
       61 gclkravvvr lvipvpehyr hpgdvprrrr isprkifvgr vwhrv
//
LOCUS       XP_076546382              83 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546382
VERSION     XP_076546382.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690267.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..83
                     /product="uncharacterized protein LOC143305696 isoform X2"
                     /calculated_mol_wt=9144
     CDS             1..83
                     /gene="LOC143305696"
                     /coded_by="XM_076690267.1:244..495"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanvv nkraprmdnv
       61 ltfipgdcws lesaealegq gwk
//
LOCUS       XP_076546383              71 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546383
VERSION     XP_076546383.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690268.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..71
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..71
                     /product="uncharacterized protein LOC143305696 isoform X3"
                     /calculated_mol_wt=7804
     CDS             1..71
                     /gene="LOC143305696"
                     /coded_by="XM_076690268.1:244..459"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanvv avtagryynr
       61 vaghfsecql h
//
LOCUS       XP_076546384              70 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546384
VERSION     XP_076546384.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690269.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..70
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..70
                     /product="uncharacterized protein LOC143305696 isoform X4"
                     /calculated_mol_wt=7736
     CDS             1..70
                     /gene="LOC143305696"
                     /coded_by="XM_076690269.1:244..456"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanve rccvcqkvgf
       61 fgftvvstwn
//
LOCUS       XP_076546385              67 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546385
VERSION     XP_076546385.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690270.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..67
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..67
                     /product="uncharacterized protein LOC143305696 isoform X5"
                     /calculated_mol_wt=7346
     CDS             1..67
                     /gene="LOC143305696"
                     /coded_by="XM_076690270.1:244..447"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanvg isakekdksk
       61 vvfnsrk
//
LOCUS       XP_076546386              65 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546386
VERSION     XP_076546386.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690271.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..65
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..65
                     /product="uncharacterized protein LOC143305696 isoform X6"
                     /calculated_mol_wt=7060
     CDS             1..65
                     /gene="LOC143305696"
                     /coded_by="XM_076690271.1:244..441"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanvd tikpaapvgh
       61 rdrsd
//
LOCUS       XP_076546387              61 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546387
VERSION     XP_076546387.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690272.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..61
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..61
                     /product="uncharacterized protein LOC143305696 isoform X7"
                     /calculated_mol_wt=6738
     CDS             1..61
                     /gene="LOC143305696"
                     /coded_by="XM_076690272.1:244..429"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanvd eptlrdvcsy
       61 d
//
LOCUS       XP_076546388              59 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305696 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546388
VERSION     XP_076546388.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690273.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..59
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..59
                     /product="uncharacterized protein LOC143305696 isoform X8"
                     /calculated_mol_wt=6274
     CDS             1..59
                     /gene="LOC143305696"
                     /coded_by="XM_076690273.1:244..423"
                     /db_xref="GeneID:143305696"
ORIGIN      
        1 mplnrhlpti lkvtsspldr mtsghrirqs fdtsvcdvqr vpvdgqanve tsspggdvt
//
LOCUS       XP_034187460             844 aa            linear   INV 03-SEP-2025
DEFINITION  glycogen phosphorylase [Osmia lignaria lignaria].
ACCESSION   XP_034187460
VERSION     XP_034187460.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331569.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="glycogen phosphorylase"
                     /calculated_mol_wt=97044
     Region          29..828
                     /region_name="GT35_Glycogen_Phosphorylase"
                     /note="glycogen phosphorylase and similar proteins;
                     cd04300"
                     /db_xref="CDD:340853"
     Site            order(29,31,33..34,36..38,61..63,194,283,286)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:340853"
     Site            order(89,91,135..137,281,284,286,293,340,342,378..379,383,
                     485,492,569..570,572..575,609,614,616,650..651,673,
                     675..678,681)
                     /site_type="active"
                     /note="active site pocket [active]"
                     /db_xref="CDD:340853"
     CDS             1..844
                     /gene="Glyp"
                     /coded_by="XM_034331569.2:355..2889"
                     /db_xref="GeneID:117607633"
ORIGIN      
        1 msasevdhek rkqisvrgiv dvenvanfkk tfnrhlhytl vkdrnvatsr dyffalahsv
       61 kdnlvsrwir tqqyyyekdp krvyylsley ymgralqntm inlgiqgacd eamyqmgldi
      121 eeleeleeda glgngglgrl aacfldsmat lgiaaygygi ryeygifaqk ikngeqieep
      181 ddwlrygnpw ekarpefmlp vnfygqvidt pegkkwvntq vifampydnp ipgyknnvvn
      241 slrlwsaksp vefnlkffnd gdyiqavidr nlaenisrvl ypndnffegk elrlkqeyfm
      301 vaatlqdiir rykaskfgsk ehhrtdfdmf pdkvaiqlnd thpslaipel mrilidvegl
      361 pwekawditt ktcaytnhtv lpealerwpt sllesilprh lqiiyhinfl hlqtvsakfp
      421 gdmdrlrrms lieeegekrv nmahlsivgs haingvaaih seilinsvfk dfyeltpekf
      481 qnktngitpr rwlllcnpnl sdiieekigs ewtvhleqla qlkqwakdpv fqrsiikikq
      541 enklrlaqil ekdygvkvnp asifdiqvkr iheykrqlln clhvitlynr ikkdpsapfv
      601 prtvmiggka apgyhlakqi iklicsvgnv inndpivgdk lkfiflenyr vtlaekiipa
      661 adlseqista gteasgtgnm kfmlngalti gtldganvem aeemgkenif ifgmtvdeve
      721 elkrkgynay dyynripelk qcidqiqggf fspnnpdefk ditnvllnwd rfysfadyes
      781 yiamqnqvsk vyqdetkwve maihniassg kfssdrtiae yareiwgvep swqrlpaphe
      841 prdi
//
LOCUS       XP_034187472             743 aa            linear   INV 03-SEP-2025
DEFINITION  establishment of cohesion [Osmia lignaria lignaria].
ACCESSION   XP_034187472
VERSION     XP_034187472.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331581.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187472.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..743
                     /product="establishment of cohesion"
                     /calculated_mol_wt=84758
     Region          530..569
                     /region_name="zf-C2H2_3"
                     /note="zinc-finger of acetyl-transferase ESCO; pfam13878"
                     /db_xref="CDD:464013"
     Region          675..742
                     /region_name="Acetyltransf_13"
                     /note="ESCO1/2 acetyl-transferase; pfam13880"
                     /db_xref="CDD:464015"
     CDS             1..743
                     /gene="eco"
                     /coded_by="XM_034331581.2:371..2602"
                     /db_xref="GeneID:117607640"
ORIGIN      
        1 mlikmdldni qndslcsten temliwdkce vspktpkrqf gkelqndite tpsrskeitl
       61 mtplrsthki nllprlskhm slgvldlveg fspnkkentl krdapdeldk savkllktde
      121 nhsvskarta lfqeqdhkak lknltistkh fyqnsnekkn ykvihklmet klqnkslseh
      181 skyhkrslrr htigginagv shgirkpkpk pktnsngkkt nnntmvindm qnsmkvqeds
      241 lkerqetnts svvpiertls pevdcnkrff ktkqspavvk isnniklkia sngevklnqk
      301 nyrgrkqahk rikttditfd atdlmvdepe iektveqsni dnilkilend wadddydtmd
      361 tliskqyani splkpvailn dvimspasel tnmtltmnik dnstlntyen intdnnnkka
      421 deiitntstm nikdistpet sesismdsdk qnpdknitdi qgistlnvse nsftdntdkk
      481 tnekepkyyp lfnkgyvakv fdeidnksmh ntkqtknwql svklnggenq ylldagqknf
      541 gatqcmecgv vyqigdpede nahlnyhnnk kvlkfpgwkt ervimedpft qsriiliepg
      601 dskqcwkkvt evleyvdrdl gltdtklsdy eqkrvylyir dkviigvlva qhittayrmi
      661 pellelncct vesspakcgi dvvwtdmnhr kqgiatklvd ilrnnfyfgy vmslddiafs
      721 ipssggkifa ekytktrnfk vys
//
LOCUS       XP_034187481             743 aa            linear   INV 03-SEP-2025
DEFINITION  establishment of cohesion [Osmia lignaria lignaria].
ACCESSION   XP_034187481
VERSION     XP_034187481.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331590.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187481.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..743
                     /product="establishment of cohesion"
                     /calculated_mol_wt=84758
     Region          530..569
                     /region_name="zf-C2H2_3"
                     /note="zinc-finger of acetyl-transferase ESCO; pfam13878"
                     /db_xref="CDD:464013"
     Region          675..742
                     /region_name="Acetyltransf_13"
                     /note="ESCO1/2 acetyl-transferase; pfam13880"
                     /db_xref="CDD:464015"
     CDS             1..743
                     /gene="eco"
                     /coded_by="XM_034331590.2:367..2598"
                     /db_xref="GeneID:117607640"
ORIGIN      
        1 mlikmdldni qndslcsten temliwdkce vspktpkrqf gkelqndite tpsrskeitl
       61 mtplrsthki nllprlskhm slgvldlveg fspnkkentl krdapdeldk savkllktde
      121 nhsvskarta lfqeqdhkak lknltistkh fyqnsnekkn ykvihklmet klqnkslseh
      181 skyhkrslrr htigginagv shgirkpkpk pktnsngkkt nnntmvindm qnsmkvqeds
      241 lkerqetnts svvpiertls pevdcnkrff ktkqspavvk isnniklkia sngevklnqk
      301 nyrgrkqahk rikttditfd atdlmvdepe iektveqsni dnilkilend wadddydtmd
      361 tliskqyani splkpvailn dvimspasel tnmtltmnik dnstlntyen intdnnnkka
      421 deiitntstm nikdistpet sesismdsdk qnpdknitdi qgistlnvse nsftdntdkk
      481 tnekepkyyp lfnkgyvakv fdeidnksmh ntkqtknwql svklnggenq ylldagqknf
      541 gatqcmecgv vyqigdpede nahlnyhnnk kvlkfpgwkt ervimedpft qsriiliepg
      601 dskqcwkkvt evleyvdrdl gltdtklsdy eqkrvylyir dkviigvlva qhittayrmi
      661 pellelncct vesspakcgi dvvwtdmnhr kqgiatklvd ilrnnfyfgy vmslddiafs
      721 ipssggkifa ekytktrnfk vys
//
LOCUS       XP_034187487             743 aa            linear   INV 03-SEP-2025
DEFINITION  establishment of cohesion [Osmia lignaria lignaria].
ACCESSION   XP_034187487
VERSION     XP_034187487.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331596.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187487.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..743
                     /product="establishment of cohesion"
                     /calculated_mol_wt=84758
     Region          530..569
                     /region_name="zf-C2H2_3"
                     /note="zinc-finger of acetyl-transferase ESCO; pfam13878"
                     /db_xref="CDD:464013"
     Region          675..742
                     /region_name="Acetyltransf_13"
                     /note="ESCO1/2 acetyl-transferase; pfam13880"
                     /db_xref="CDD:464015"
     CDS             1..743
                     /gene="eco"
                     /coded_by="XM_034331596.2:371..2602"
                     /db_xref="GeneID:117607640"
ORIGIN      
        1 mlikmdldni qndslcsten temliwdkce vspktpkrqf gkelqndite tpsrskeitl
       61 mtplrsthki nllprlskhm slgvldlveg fspnkkentl krdapdeldk savkllktde
      121 nhsvskarta lfqeqdhkak lknltistkh fyqnsnekkn ykvihklmet klqnkslseh
      181 skyhkrslrr htigginagv shgirkpkpk pktnsngkkt nnntmvindm qnsmkvqeds
      241 lkerqetnts svvpiertls pevdcnkrff ktkqspavvk isnniklkia sngevklnqk
      301 nyrgrkqahk rikttditfd atdlmvdepe iektveqsni dnilkilend wadddydtmd
      361 tliskqyani splkpvailn dvimspasel tnmtltmnik dnstlntyen intdnnnkka
      421 deiitntstm nikdistpet sesismdsdk qnpdknitdi qgistlnvse nsftdntdkk
      481 tnekepkyyp lfnkgyvakv fdeidnksmh ntkqtknwql svklnggenq ylldagqknf
      541 gatqcmecgv vyqigdpede nahlnyhnnk kvlkfpgwkt ervimedpft qsriiliepg
      601 dskqcwkkvt evleyvdrdl gltdtklsdy eqkrvylyir dkviigvlva qhittayrmi
      661 pellelncct vesspakcgi dvvwtdmnhr kqgiatklvd ilrnnfyfgy vmslddiafs
      721 ipssggkifa ekytktrnfk vys
//
LOCUS       XP_034187499             626 aa            linear   INV 03-SEP-2025
DEFINITION  putative ATP-dependent RNA helicase DDX43 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187499
VERSION     XP_034187499.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331608.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187499.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..626
                     /product="putative ATP-dependent RNA helicase DDX43"
                     /calculated_mol_wt=71807
     Region          54..117
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          163..623
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(245,247..249,252,271..277,314,318)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350716"
     CDS             1..626
                     /gene="LOC117607658"
                     /coded_by="XM_034331608.2:272..2152"
                     /db_xref="GeneID:117607658"
ORIGIN      
        1 mdnyqrtytv snnkymkrgn qeryhrnnyr neenqynnsr ysnsdcesek fiihinsdkv
       61 gkligrsgan ikdlqsktnt kihidrstgn dttavtiigs keaqqhaqne iedllkdssy
      121 vstevkneqt eqfeqedkpp idfttfdwtk ageecdayrk ekwskfapvi knfykehpev
      181 anmtkeqvah irqtnnniev rymfeneekc sesskdfpip npietfeqaf edypeileei
      241 rkqgfakpsp iqcqawpvll sgkdligiaq tgtgktlafl lpalihiegq etprsertgp
      301 nilvmaptre lalqiekevn kysyhgikav ciygggsrke qvnivtkgve iviatpgrln
      361 dlveanilni ssvtylvlde adrmldmgfe pqirktlldi rpdrqtvmts atwpqgvrrl
      421 aqsymqnpiq vfvgsldlat vhtvmqkiyi vdeeeksdrm yqffrematt dkvivffgkk
      481 crvddiasdl alqgvncqsi hggreqcdre qaledlktge vqillatdva srgidiedvt
      541 hvlnydfprd ieeyvhrvgr tgragrtges itfmtrkdwa lakelitlle eanqeipeel
      601 fkmaerfear rdkrdfpqrr ggnrry
//
LOCUS       XP_034187508             626 aa            linear   INV 03-SEP-2025
DEFINITION  putative ATP-dependent RNA helicase DDX43 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187508
VERSION     XP_034187508.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331617.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187508.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..626
                     /product="putative ATP-dependent RNA helicase DDX43"
                     /calculated_mol_wt=71807
     Region          54..117
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          163..623
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(245,247..249,252,271..277,314,318)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350716"
     CDS             1..626
                     /gene="LOC117607658"
                     /coded_by="XM_034331617.2:248..2128"
                     /db_xref="GeneID:117607658"
ORIGIN      
        1 mdnyqrtytv snnkymkrgn qeryhrnnyr neenqynnsr ysnsdcesek fiihinsdkv
       61 gkligrsgan ikdlqsktnt kihidrstgn dttavtiigs keaqqhaqne iedllkdssy
      121 vstevkneqt eqfeqedkpp idfttfdwtk ageecdayrk ekwskfapvi knfykehpev
      181 anmtkeqvah irqtnnniev rymfeneekc sesskdfpip npietfeqaf edypeileei
      241 rkqgfakpsp iqcqawpvll sgkdligiaq tgtgktlafl lpalihiegq etprsertgp
      301 nilvmaptre lalqiekevn kysyhgikav ciygggsrke qvnivtkgve iviatpgrln
      361 dlveanilni ssvtylvlde adrmldmgfe pqirktlldi rpdrqtvmts atwpqgvrrl
      421 aqsymqnpiq vfvgsldlat vhtvmqkiyi vdeeeksdrm yqffrematt dkvivffgkk
      481 crvddiasdl alqgvncqsi hggreqcdre qaledlktge vqillatdva srgidiedvt
      541 hvlnydfprd ieeyvhrvgr tgragrtges itfmtrkdwa lakelitlle eanqeipeel
      601 fkmaerfear rdkrdfpqrr ggnrry
//
LOCUS       XP_034187517             626 aa            linear   INV 03-SEP-2025
DEFINITION  putative ATP-dependent RNA helicase DDX43 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187517
VERSION     XP_034187517.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331626.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187517.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..626
                     /product="putative ATP-dependent RNA helicase DDX43"
                     /calculated_mol_wt=71807
     Region          54..117
                     /region_name="KH-I"
                     /note="K homology (KH) RNA-binding domain, type I;
                     cl00098"
                     /db_xref="CDD:469614"
     Region          163..623
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(245,247..249,252,271..277,314,318)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350716"
     CDS             1..626
                     /gene="LOC117607658"
                     /coded_by="XM_034331626.2:210..2090"
                     /db_xref="GeneID:117607658"
ORIGIN      
        1 mdnyqrtytv snnkymkrgn qeryhrnnyr neenqynnsr ysnsdcesek fiihinsdkv
       61 gkligrsgan ikdlqsktnt kihidrstgn dttavtiigs keaqqhaqne iedllkdssy
      121 vstevkneqt eqfeqedkpp idfttfdwtk ageecdayrk ekwskfapvi knfykehpev
      181 anmtkeqvah irqtnnniev rymfeneekc sesskdfpip npietfeqaf edypeileei
      241 rkqgfakpsp iqcqawpvll sgkdligiaq tgtgktlafl lpalihiegq etprsertgp
      301 nilvmaptre lalqiekevn kysyhgikav ciygggsrke qvnivtkgve iviatpgrln
      361 dlveanilni ssvtylvlde adrmldmgfe pqirktlldi rpdrqtvmts atwpqgvrrl
      421 aqsymqnpiq vfvgsldlat vhtvmqkiyi vdeeeksdrm yqffrematt dkvivffgkk
      481 crvddiasdl alqgvncqsi hggreqcdre qaledlktge vqillatdva srgidiedvt
      541 hvlnydfprd ieeyvhrvgr tgragrtges itfmtrkdwa lakelitlle eanqeipeel
      601 fkmaerfear rdkrdfpqrr ggnrry
//
LOCUS       XP_034187530             595 aa            linear   INV 03-SEP-2025
DEFINITION  2',5'-phosphodiesterase 12 [Osmia lignaria lignaria].
ACCESSION   XP_034187530
VERSION     XP_034187530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331639.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="2',5'-phosphodiesterase 12"
                     /calculated_mol_wt=68694
     Region          281..593
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(283,333,437,482,484,546,583..584)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197331"
     Site            order(333,583)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197331"
     Site            order(437,484,584)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197331"
     CDS             1..595
                     /gene="LOC117607675"
                     /coded_by="XM_034331639.2:177..1964"
                     /db_xref="GeneID:117607675"
ORIGIN      
        1 msilfrnlhl kyltsfltsi stvyqgfckr ysqimvqlni nealliheeg stkfdmllry
       61 inpalnidrr fnfhrsvdep innflqridm niksyiikks krknkkavnl eanvmdnike
      121 nnikfmlndc vlngsltcqt ilensseikl iifgeeyivk hnvpfvtkme lplsilidfp
      181 iypskfetmn vnkslstfnw ykhennqwvh vgegflytpn ksdlgcklkv tciprnttqi
      241 gptieivssd ivqpgpglcp fdtrhaftkn klsgksfrlt synilanvys etslskdtly
      301 pycpqyalsm dyrkllilke ligyntdiic lqevdskvye ndlmlslnal nydgvfnlkn
      361 dlreglvify nrerfdklns dysvisqgte ldefkdvwlq ienenvkqaf lnrntiiqtv
      421 ilkskenpei lvignthlyf rpeadhirll qayygltylh sfagkvkken pecnvsiiyc
      481 gdfnsvpdsa vyrlmtqkhi lnyyndwhad sdqrinlsik hdvnlssacg tpkytnytgt
      541 fsgcldyify qmdhleveqv iplpseeels lytglpsvvf psdhislcvd lkwsk
//
LOCUS       XP_034187551             531 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein 39-like protein Caper isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034187551
VERSION     XP_034187551.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..531
                     /product="RNA-binding protein 39-like protein Caper
                     isoform X1"
                     /calculated_mol_wt=60156
     Region          89..514
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     CDS             1..531
                     /gene="Caper"
                     /coded_by="XM_034331660.2:186..1781"
                     /db_xref="GeneID:117607684"
ORIGIN      
        1 mrgisesspt rtmaedldve amleapykkg eqdssskdks skengssrks sgknkhrsrs
       61 rsrsrdrrek drrdrdrdrd rdrdrdrdrd rdrdrdrdrr rrsrsrdrrd rdrdrdndrd
      121 rdrrdrdrdr drrrkrsltp itlprarppf gkgvsplgir ndeltpeerd artvfcmqls
      181 qrirardled ffssvgkvqd vrlitcnktr rfkgiayvef kdpesvtlal glsgqkllgv
      241 pivvqhtqae knrmgnsmpn lmpkgqtgpm rlyvgslhfn itedmlrgif epfgkidniq
      301 limdpetgrs kgygfltfrn addakkaleq lngfelagrp mkvgnvtert dliqgpslld
      361 tdeldrsgid lgatgrlqlm fklaegtgle ippaaanaln mapvmttpqp ppqaappiat
      421 qcfmlsnmfd pqnetnpnwa keirddviee cnkhggvlhv yvdqaspqgn vyvkcpsiat
      481 avaavnslhg rwfagrvita ayvpvvnyhs lfpdamtalq llvpsaprrg m
//
LOCUS       XP_034187570             519 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein 39-like protein Caper isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034187570
VERSION     XP_034187570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331679.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="RNA-binding protein 39-like protein Caper
                     isoform X2"
                     /calculated_mol_wt=58853
     Region          77..502
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     CDS             1..519
                     /gene="Caper"
                     /coded_by="XM_034331679.2:155..1714"
                     /db_xref="GeneID:117607684"
ORIGIN      
        1 maedldveam leapykkgeq dssskdkssk engssrkssg knkhrsrsrs rsrdrrekdr
       61 rdrdrdrdrd rdrdrdrdrd rdrdrdrrrr srsrdrrdrd rdrdndrdrd rrdrdrdrdr
      121 rrkrsltpit lprarppfgk gvsplgirnd eltpeerdar tvfcmqlsqr irardledff
      181 ssvgkvqdvr litcnktrrf kgiayvefkd pesvtlalgl sgqkllgvpi vvqhtqaekn
      241 rmgnsmpnlm pkgqtgpmrl yvgslhfnit edmlrgifep fgkidniqli mdpetgrskg
      301 ygfltfrnad dakkaleqln gfelagrpmk vgnvtertdl iqgpslldtd eldrsgidlg
      361 atgrlqlmfk laegtgleip paaanalnma pvmttpqppp qaappiatqc fmlsnmfdpq
      421 netnpnwake irddvieecn khggvlhvyv dqaspqgnvy vkcpsiatav aavnslhgrw
      481 fagrvitaay vpvvnyhslf pdamtalqll vpsaprrgm
//
LOCUS       XP_034187578             507 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein 39-like protein Caper isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034187578
VERSION     XP_034187578.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="RNA-binding protein 39-like protein Caper
                     isoform X3"
                     /calculated_mol_wt=57662
     Region          65..490
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     CDS             1..507
                     /gene="Caper"
                     /coded_by="XM_034331687.2:332..1855"
                     /db_xref="GeneID:117607684"
ORIGIN      
        1 mhlkyeeqds sskdkssken gssrkssgkn khrsrsrsrs rdrrekdrrd rdrdrdrdrd
       61 rdrdrdrdrd rdrdrrrrsr srdrrdrdrd rdndrdrdrr drdrdrdrrr krsltpitlp
      121 rarppfgkgv splgirndel tpeerdartv fcmqlsqrir ardledffss vgkvqdvrli
      181 tcnktrrfkg iayvefkdpe svtlalglsg qkllgvpivv qhtqaeknrm gnsmpnlmpk
      241 gqtgpmrlyv gslhfnited mlrgifepfg kidniqlimd petgrskgyg fltfrnadda
      301 kkaleqlngf elagrpmkvg nvtertdliq gpslldtdel drsgidlgat grlqlmfkla
      361 egtgleippa aanalnmapv mttpqpppqa appiatqcfm lsnmfdpqne tnpnwakeir
      421 ddvieecnkh ggvlhvyvdq aspqgnvyvk cpsiatavaa vnslhgrwfa grvitaayvp
      481 vvnyhslfpd amtalqllvp saprrgm
//
LOCUS       XP_034187589             507 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein 39-like protein Caper isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034187589
VERSION     XP_034187589.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331698.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="RNA-binding protein 39-like protein Caper
                     isoform X3"
                     /calculated_mol_wt=57662
     Region          65..490
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     CDS             1..507
                     /gene="Caper"
                     /coded_by="XM_034331698.2:209..1732"
                     /db_xref="GeneID:117607684"
ORIGIN      
        1 mhlkyeeqds sskdkssken gssrkssgkn khrsrsrsrs rdrrekdrrd rdrdrdrdrd
       61 rdrdrdrdrd rdrdrrrrsr srdrrdrdrd rdndrdrdrr drdrdrdrrr krsltpitlp
      121 rarppfgkgv splgirndel tpeerdartv fcmqlsqrir ardledffss vgkvqdvrli
      181 tcnktrrfkg iayvefkdpe svtlalglsg qkllgvpivv qhtqaeknrm gnsmpnlmpk
      241 gqtgpmrlyv gslhfnited mlrgifepfg kidniqlimd petgrskgyg fltfrnadda
      301 kkaleqlngf elagrpmkvg nvtertdliq gpslldtdel drsgidlgat grlqlmfkla
      361 egtgleippa aanalnmapv mttpqpppqa appiatqcfm lsnmfdpqne tnpnwakeir
      421 ddvieecnkh ggvlhvyvdq aspqgnvyvk cpsiatavaa vnslhgrwfa grvitaayvp
      481 vvnyhslfpd amtalqllvp saprrgm
//
LOCUS       XP_034187603             336 aa            linear   INV 03-SEP-2025
DEFINITION  galectin-8 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034187603
VERSION     XP_034187603.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331712.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="galectin-8 isoform X1"
                     /calculated_mol_wt=38181
     Region          37..156
                     /region_name="GLECT"
                     /note="Galectin/galactose-binding lectin. This domain
                     exclusively binds beta-galactosides, such as lactose, and
                     does not require metal ions for activity. GLECT domains
                     occur as homodimers or tandemly repeated domains. They are
                     developmentally regulated and may...; cd00070"
                     /db_xref="CDD:238025"
     Site            order(42..44,47,50,119..121,126,128,131)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(74,76,78,85,87,94,97,99)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     Site            order(196..200,326..330)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            196..200
                     /site_type="active"
                     /note="dimerization swap strand [active]"
                     /db_xref="CDD:238025"
     Region          204..328
                     /region_name="Gal-bind_lectin"
                     /note="Galactoside-binding lectin; pfam00337"
                     /db_xref="CDD:459768"
     Site            order(205..207,210,213,289..291,296,298,301)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(242,244,246,257,259,266,269,271)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     CDS             1..336
                     /gene="LOC117607702"
                     /coded_by="XM_034331712.2:310..1320"
                     /db_xref="GeneID:117607702"
ORIGIN      
        1 msvnenkifv qtkenavref sfvdedtlpl nvsapvllqp lkptsaivvt gyipadalrf
       61 svnllcktgn ialhfnarld rgyvvrntkf kgcweeeetc spsghtafrr nsyvhilifc
      121 tanafqiavn gehfcafsyr mpleditnle ingsledtrf rqlelfvypd pklcrpsnil
      181 slkveqpive clsvpitvgi esefkpgtrl fiagrlkllp hsfyvnlqkg kaiyphpiia
      241 lhlnprfsyg ssapyvvmnc wmngswehee rhhghlswmp grdflltirc ehevytiwlg
      301 dkmigefkhr lqpsivdtlk isgdivlhel amtysn
//
LOCUS       XP_034187613             320 aa            linear   INV 03-SEP-2025
DEFINITION  galectin-8 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034187613
VERSION     XP_034187613.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331722.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="galectin-8 isoform X2"
                     /calculated_mol_wt=36485
     Region          21..140
                     /region_name="GLECT"
                     /note="Galectin/galactose-binding lectin. This domain
                     exclusively binds beta-galactosides, such as lactose, and
                     does not require metal ions for activity. GLECT domains
                     occur as homodimers or tandemly repeated domains. They are
                     developmentally regulated and may...; cd00070"
                     /db_xref="CDD:238025"
     Site            order(26..28,31,34,103..105,110,112,115)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(58,60,62,69,71,78,81,83)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     Site            order(180..184,310..314)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            180..184
                     /site_type="active"
                     /note="dimerization swap strand [active]"
                     /db_xref="CDD:238025"
     Region          188..312
                     /region_name="Gal-bind_lectin"
                     /note="Galactoside-binding lectin; pfam00337"
                     /db_xref="CDD:459768"
     Site            order(189..191,194,197,273..275,280,282,285)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(226,228,230,241,243,250,253,255)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     CDS             1..320
                     /gene="LOC117607702"
                     /coded_by="XM_034331722.2:296..1258"
                     /db_xref="GeneID:117607702"
ORIGIN      
        1 mlkirirtlt sfyynvsapv llqplkptsa ivvtgyipad alrfsvnllc ktgnialhfn
       61 arldrgyvvr ntkfkgcwee eetcspsght afrrnsyvhi lifctanafq iavngehfca
      121 fsyrmpledi tnleingsle dtrfrqlelf vypdpklcrp snilslkveq piveclsvpi
      181 tvgiesefkp gtrlfiagrl kllphsfyvn lqkgkaiyph piialhlnpr fsygssapyv
      241 vmncwmngsw eheerhhghl swmpgrdfll tircehevyt iwlgdkmige fkhrlqpsiv
      301 dtlkisgdiv lhelamtysn
//
LOCUS       XP_034187622             320 aa            linear   INV 03-SEP-2025
DEFINITION  galectin-8 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034187622
VERSION     XP_034187622.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331731.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="galectin-8 isoform X2"
                     /calculated_mol_wt=36485
     Region          21..140
                     /region_name="GLECT"
                     /note="Galectin/galactose-binding lectin. This domain
                     exclusively binds beta-galactosides, such as lactose, and
                     does not require metal ions for activity. GLECT domains
                     occur as homodimers or tandemly repeated domains. They are
                     developmentally regulated and may...; cd00070"
                     /db_xref="CDD:238025"
     Site            order(26..28,31,34,103..105,110,112,115)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(58,60,62,69,71,78,81,83)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     Site            order(180..184,310..314)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            180..184
                     /site_type="active"
                     /note="dimerization swap strand [active]"
                     /db_xref="CDD:238025"
     Region          188..312
                     /region_name="Gal-bind_lectin"
                     /note="Galactoside-binding lectin; pfam00337"
                     /db_xref="CDD:459768"
     Site            order(189..191,194,197,273..275,280,282,285)
                     /site_type="other"
                     /note="putative alternate dimerization interface
                     [polypeptide binding]"
                     /db_xref="CDD:238025"
     Site            order(226,228,230,241,243,250,253,255)
                     /site_type="other"
                     /note="sugar binding pocket [chemical binding]"
                     /db_xref="CDD:238025"
     CDS             1..320
                     /gene="LOC117607702"
                     /coded_by="XM_034331731.2:492..1454"
                     /db_xref="GeneID:117607702"
ORIGIN      
        1 mlkirirtlt sfyynvsapv llqplkptsa ivvtgyipad alrfsvnllc ktgnialhfn
       61 arldrgyvvr ntkfkgcwee eetcspsght afrrnsyvhi lifctanafq iavngehfca
      121 fsyrmpledi tnleingsle dtrfrqlelf vypdpklcrp snilslkveq piveclsvpi
      181 tvgiesefkp gtrlfiagrl kllphsfyvn lqkgkaiyph piialhlnpr fsygssapyv
      241 vmncwmngsw eheerhhghl swmpgrdfll tircehevyt iwlgdkmige fkhrlqpsiv
      301 dtlkisgdiv lhelamtysn
//
LOCUS       XP_034187636             270 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607715 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187636
VERSION     XP_034187636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="uncharacterized protein LOC117607715 isoform X1"
                     /calculated_mol_wt=31542
     CDS             1..270
                     /gene="LOC117607715"
                     /coded_by="XM_034331745.2:103..915"
                     /db_xref="GeneID:117607715"
ORIGIN      
        1 mdskrhdrss ksntdsppap psppmysasy ttydgyknrl ssrrspspse yrssrtsrnd
       61 spqdrrrrrr stsksppsrs hkrsrspdrd kdrdrdrdrs rkysrrdrsr srsrgrsrsr
      121 drrrsrsrnr srdrhrgres rryhrapsye sdtaednndt gvsqpvvplq snafkndgsf
      181 memfkkmqeq mqpaqkptts vleektvvpp plmvgkrrgg rilktgmvak pkteqtveqp
      241 kdawslymae vkkyrevccq eedntrplvk
//
LOCUS       XP_034187644             270 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607715 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187644
VERSION     XP_034187644.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331753.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="uncharacterized protein LOC117607715 isoform X1"
                     /calculated_mol_wt=31542
     CDS             1..270
                     /gene="LOC117607715"
                     /coded_by="XM_034331753.2:196..1008"
                     /db_xref="GeneID:117607715"
ORIGIN      
        1 mdskrhdrss ksntdsppap psppmysasy ttydgyknrl ssrrspspse yrssrtsrnd
       61 spqdrrrrrr stsksppsrs hkrsrspdrd kdrdrdrdrs rkysrrdrsr srsrgrsrsr
      121 drrrsrsrnr srdrhrgres rryhrapsye sdtaednndt gvsqpvvplq snafkndgsf
      181 memfkkmqeq mqpaqkptts vleektvvpp plmvgkrrgg rilktgmvak pkteqtveqp
      241 kdawslymae vkkyrevccq eedntrplvk
//
LOCUS       XP_034187652              92 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607715 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187652
VERSION     XP_034187652.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331761.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187652.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..92
                     /product="uncharacterized protein LOC117607715 isoform X2"
                     /calculated_mol_wt=10716
     Region          22..90
                     /region_name="Cyt_c_Oxidase_VIb"
                     /note="Cytochrome c oxidase subunit VIb. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cd00926"
                     /db_xref="CDD:238466"
     Site            order(22..24,33..36,65,68..74)
                     /site_type="other"
                     /note="Subunit VIb/II interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            33
                     /site_type="other"
                     /note="Subunit VIb/I interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(37,40,89)
                     /site_type="other"
                     /note="Subunit VIb/III interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(53,55..60)
                     /site_type="other"
                     /note="Subunit VIb/VIb interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(82,85..87)
                     /site_type="other"
                     /note="Subunit VIb/VIa interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     CDS             1..92
                     /gene="LOC117607715"
                     /coded_by="XM_034331761.2:81..359"
                     /db_xref="GeneID:117607715"
ORIGIN      
        1 mvkytnlgtk peidagidvk pqtapydprf pnqnqtkycy vsyvdfhrck krhgedydtc
       61 kyfkkvyqam cpnawvekwd eqvengtfag hi
//
LOCUS       XP_034187668              92 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607715 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187668
VERSION     XP_034187668.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331777.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187668.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..92
                     /product="uncharacterized protein LOC117607715 isoform X2"
                     /calculated_mol_wt=10716
     Region          22..90
                     /region_name="Cyt_c_Oxidase_VIb"
                     /note="Cytochrome c oxidase subunit VIb. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cd00926"
                     /db_xref="CDD:238466"
     Site            order(22..24,33..36,65,68..74)
                     /site_type="other"
                     /note="Subunit VIb/II interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            33
                     /site_type="other"
                     /note="Subunit VIb/I interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(37,40,89)
                     /site_type="other"
                     /note="Subunit VIb/III interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(53,55..60)
                     /site_type="other"
                     /note="Subunit VIb/VIb interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(82,85..87)
                     /site_type="other"
                     /note="Subunit VIb/VIa interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     CDS             1..92
                     /gene="LOC117607715"
                     /coded_by="XM_034331777.2:78..356"
                     /db_xref="GeneID:117607715"
ORIGIN      
        1 mvkytnlgtk peidagidvk pqtapydprf pnqnqtkycy vsyvdfhrck krhgedydtc
       61 kyfkkvyqam cpnawvekwd eqvengtfag hi
//
LOCUS       XP_034187661              92 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607715 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187661
VERSION     XP_034187661.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187661.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..92
                     /product="uncharacterized protein LOC117607715 isoform X2"
                     /calculated_mol_wt=10716
     Region          22..90
                     /region_name="Cyt_c_Oxidase_VIb"
                     /note="Cytochrome c oxidase subunit VIb. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cd00926"
                     /db_xref="CDD:238466"
     Site            order(22..24,33..36,65,68..74)
                     /site_type="other"
                     /note="Subunit VIb/II interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            33
                     /site_type="other"
                     /note="Subunit VIb/I interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(37,40,89)
                     /site_type="other"
                     /note="Subunit VIb/III interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(53,55..60)
                     /site_type="other"
                     /note="Subunit VIb/VIb interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(82,85..87)
                     /site_type="other"
                     /note="Subunit VIb/VIa interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     CDS             1..92
                     /gene="LOC117607715"
                     /coded_by="XM_034331770.2:154..432"
                     /db_xref="GeneID:117607715"
ORIGIN      
        1 mvkytnlgtk peidagidvk pqtapydprf pnqnqtkycy vsyvdfhrck krhgedydtc
       61 kyfkkvyqam cpnawvekwd eqvengtfag hi
//
LOCUS       XP_034187677              92 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607715 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187677
VERSION     XP_034187677.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331786.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187677.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..92
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..92
                     /product="uncharacterized protein LOC117607715 isoform X2"
                     /calculated_mol_wt=10716
     Region          22..90
                     /region_name="Cyt_c_Oxidase_VIb"
                     /note="Cytochrome c oxidase subunit VIb. Cytochrome c
                     oxidase (CcO), the terminal oxidase in the respiratory
                     chains of eukaryotes and most bacteria, is a multi-chain
                     transmembrane protein located in the inner membrane of
                     mitochondria and the cell membrane of...; cd00926"
                     /db_xref="CDD:238466"
     Site            order(22..24,33..36,65,68..74)
                     /site_type="other"
                     /note="Subunit VIb/II interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            33
                     /site_type="other"
                     /note="Subunit VIb/I interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(37,40,89)
                     /site_type="other"
                     /note="Subunit VIb/III interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(53,55..60)
                     /site_type="other"
                     /note="Subunit VIb/VIb interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     Site            order(82,85..87)
                     /site_type="other"
                     /note="Subunit VIb/VIa interface [polypeptide binding]"
                     /db_xref="CDD:238466"
     CDS             1..92
                     /gene="LOC117607715"
                     /coded_by="XM_034331786.2:152..430"
                     /db_xref="GeneID:117607715"
ORIGIN      
        1 mvkytnlgtk peidagidvk pqtapydprf pnqnqtkycy vsyvdfhrck krhgedydtc
       61 kyfkkvyqam cpnawvekwd eqvengtfag hi
//
LOCUS       XP_034187766            2003 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607777 [Osmia lignaria lignaria].
ACCESSION   XP_034187766
VERSION     XP_034187766.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331875.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187766.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2003
                     /product="uncharacterized protein LOC117607777"
                     /calculated_mol_wt=225947
     Region          201..>291
                     /region_name="OmpH"
                     /note="Outer membrane protein (OmpH-like); pfam03938"
                     /db_xref="CDD:461098"
     Region          <1015..>1199
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1699..>1845
                     /region_name="Kti12"
                     /note="tRNA uridine 5-carbamoylmethylation protein Kti12
                     (Killer toxin insensitivity protein) [Translation,
                     ribosomal structure and biogenesis, Defense mechanisms];
                     COG4088"
                     /db_xref="CDD:443264"
     CDS             1..2003
                     /gene="LOC117607777"
                     /coded_by="XM_034331875.2:323..6334"
                     /db_xref="GeneID:117607777"
ORIGIN      
        1 mqnwnqwqls agavatpmpq ppvgyatpga dpmammqaym qyynqpapsg ytaeqwaaaq
       61 qqnwaqwqqw qqqyqqwqaq ygekyqetmk hisaqnlnla sqvpqlpvvq pppplpkeda
      121 kpplppttmn tyqftsmppp hqnnlplfpv kqnantsvqq gnvqncpqnp plppnqpplp
      181 pdssnnsken nnvigkrssn aisespsakk lkvedeelte aektfdaqfk qweeqfnkwk
      241 qqnanhpdkt qykqyeakwt swreklierr eqmrrkreqq kqvtakaeve knkslpgddk
      301 imnilssten qglinnllgi gktlgltgkq nanvpppppp ppatettvsn vqttmtsssq
      361 atsqsvpqls vmnmmnsspw nsqqwtpqyn tnvsnfpnfq smsgvsqvpf ngpptqmppp
      421 nftqpppnfs svpnfsqppp nfanndsrqn qnarpsvppn iqgrpppfgs nnnsgpdgsh
      481 lhnerpgqgg pinnfesvsh pnnfndsnre kdgvplerfa rdgvadqtss qfrsndmygq
      541 rnenyklnde rgsesdqfrr edrnyqdfgg nqfnqhkmnf nndrfgagnd rfgsgnnrfg
      601 pgndrfgpgn drfgsgndrf gsgdnrftgn drfgpgmdrm vqgnepvndr fgsnneppyg
      661 pggdrfnrnn ldrfdqkdlg drrdgfpvap rnfnrnnqfe psdkfapelk klmekrraai
      721 dvfkpsflds dknssvgsls esfkkitges pfmktssnnn fgprgssnfg psgpgnfrmp
      781 gdfkppgnad fgprcptgig rnnsfelrdg gtapfrgpts spqqefiite prpemnkenk
      841 nesvtgveqp vvilndqnad svqkdgsegy pvqneiplle kppwidaqlp entslqkdkr
      901 ddnlptnynp sehipqdtqn ainkstegkq elsgsdnkki ealpfmgend pkpedlnmep
      961 ppelpnlgpv sdtnevadpm rgtnepydvk esslkpfpnn pecfepretf dstlgprgmq
     1021 fkpntpftgp rsstdnrfpa feprgpnsfp fnpsalnpyp lgprglndgq fgprgpndtq
     1081 fgqrgvndgl fgprgpndgp fgprgptgpf gprgstenqf gprgpnngqf gprgsnngqf
     1141 gprydsqfgl rrsndgqfap rgpidgqlgp qgpnnrqfgp grqndapfdn cgqftprvps
     1201 ethrfpgpnd rlfgsrrneg pyqrsptgak gtnngqfsnm sdvygsqnsn dayfgsrgpm
     1261 dgpndnrqcd irgigefrsk gpvdnnscpk lrensdaags nvrgdggvye atnqmrieqn
     1321 isqgqfdtdd msnpgwrksy pknsfgdtdq rsrrnfcfid kpllaqnetf nqdtisnver
     1381 rsplnvnkss ipdrseksma dltagdyksg ygesssgsdy tkfggpsmfm krsidnrqsq
     1441 vrcpavkefc iekqfnynhg gattdkkfie hipakvidya httrskiqdh ltpvqcfdyg
     1501 hgnlkpsvpd hevypkkdfr nweeneqnlk eytekirrye yyaektdsrr srnynkkrns
     1561 edsyderkpe rerrekednk gkgkvdrifd hmsdkdqdir yeknsnkert rekcetshke
     1621 nkendkdedr skgnknwqen tskniidtkp pdtsvtdpqh nvleptqktl elaktpnctm
     1681 vddllcppgr qnrppkiaii lrgppgsgks fvaklikdke veqggsapri lslddyflve
     1741 keiestddng kkitikemvy eyeeameqsy itslvkafkk nitdgffnfi ildcinekis
     1801 dyeemwsfak tkgfkvyvce memdlqiclk rnihnrtede inriidyfep tpsyhqkldv
     1861 nsmlqeqaie evhmedseet qersehhned sqdsqddiqd vtgvskwerm eaedkldrld
     1921 glakkknegk vqtmkdflqv pdyynmedts gkkrvrwadl eerkeqekmr avgfvvghtn
     1981 wdrmmdptkg gsaltrtkff sls
//
LOCUS       XP_034187774            2003 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607777 [Osmia lignaria lignaria].
ACCESSION   XP_034187774
VERSION     XP_034187774.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331883.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187774.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2003
                     /product="uncharacterized protein LOC117607777"
                     /calculated_mol_wt=225947
     Region          201..>291
                     /region_name="OmpH"
                     /note="Outer membrane protein (OmpH-like); pfam03938"
                     /db_xref="CDD:461098"
     Region          <1015..>1199
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1699..>1845
                     /region_name="Kti12"
                     /note="tRNA uridine 5-carbamoylmethylation protein Kti12
                     (Killer toxin insensitivity protein) [Translation,
                     ribosomal structure and biogenesis, Defense mechanisms];
                     COG4088"
                     /db_xref="CDD:443264"
     CDS             1..2003
                     /gene="LOC117607777"
                     /coded_by="XM_034331883.2:278..6289"
                     /db_xref="GeneID:117607777"
ORIGIN      
        1 mqnwnqwqls agavatpmpq ppvgyatpga dpmammqaym qyynqpapsg ytaeqwaaaq
       61 qqnwaqwqqw qqqyqqwqaq ygekyqetmk hisaqnlnla sqvpqlpvvq pppplpkeda
      121 kpplppttmn tyqftsmppp hqnnlplfpv kqnantsvqq gnvqncpqnp plppnqpplp
      181 pdssnnsken nnvigkrssn aisespsakk lkvedeelte aektfdaqfk qweeqfnkwk
      241 qqnanhpdkt qykqyeakwt swreklierr eqmrrkreqq kqvtakaeve knkslpgddk
      301 imnilssten qglinnllgi gktlgltgkq nanvpppppp ppatettvsn vqttmtsssq
      361 atsqsvpqls vmnmmnsspw nsqqwtpqyn tnvsnfpnfq smsgvsqvpf ngpptqmppp
      421 nftqpppnfs svpnfsqppp nfanndsrqn qnarpsvppn iqgrpppfgs nnnsgpdgsh
      481 lhnerpgqgg pinnfesvsh pnnfndsnre kdgvplerfa rdgvadqtss qfrsndmygq
      541 rnenyklnde rgsesdqfrr edrnyqdfgg nqfnqhkmnf nndrfgagnd rfgsgnnrfg
      601 pgndrfgpgn drfgsgndrf gsgdnrftgn drfgpgmdrm vqgnepvndr fgsnneppyg
      661 pggdrfnrnn ldrfdqkdlg drrdgfpvap rnfnrnnqfe psdkfapelk klmekrraai
      721 dvfkpsflds dknssvgsls esfkkitges pfmktssnnn fgprgssnfg psgpgnfrmp
      781 gdfkppgnad fgprcptgig rnnsfelrdg gtapfrgpts spqqefiite prpemnkenk
      841 nesvtgveqp vvilndqnad svqkdgsegy pvqneiplle kppwidaqlp entslqkdkr
      901 ddnlptnynp sehipqdtqn ainkstegkq elsgsdnkki ealpfmgend pkpedlnmep
      961 ppelpnlgpv sdtnevadpm rgtnepydvk esslkpfpnn pecfepretf dstlgprgmq
     1021 fkpntpftgp rsstdnrfpa feprgpnsfp fnpsalnpyp lgprglndgq fgprgpndtq
     1081 fgqrgvndgl fgprgpndgp fgprgptgpf gprgstenqf gprgpnngqf gprgsnngqf
     1141 gprydsqfgl rrsndgqfap rgpidgqlgp qgpnnrqfgp grqndapfdn cgqftprvps
     1201 ethrfpgpnd rlfgsrrneg pyqrsptgak gtnngqfsnm sdvygsqnsn dayfgsrgpm
     1261 dgpndnrqcd irgigefrsk gpvdnnscpk lrensdaags nvrgdggvye atnqmrieqn
     1321 isqgqfdtdd msnpgwrksy pknsfgdtdq rsrrnfcfid kpllaqnetf nqdtisnver
     1381 rsplnvnkss ipdrseksma dltagdyksg ygesssgsdy tkfggpsmfm krsidnrqsq
     1441 vrcpavkefc iekqfnynhg gattdkkfie hipakvidya httrskiqdh ltpvqcfdyg
     1501 hgnlkpsvpd hevypkkdfr nweeneqnlk eytekirrye yyaektdsrr srnynkkrns
     1561 edsyderkpe rerrekednk gkgkvdrifd hmsdkdqdir yeknsnkert rekcetshke
     1621 nkendkdedr skgnknwqen tskniidtkp pdtsvtdpqh nvleptqktl elaktpnctm
     1681 vddllcppgr qnrppkiaii lrgppgsgks fvaklikdke veqggsapri lslddyflve
     1741 keiestddng kkitikemvy eyeeameqsy itslvkafkk nitdgffnfi ildcinekis
     1801 dyeemwsfak tkgfkvyvce memdlqiclk rnihnrtede inriidyfep tpsyhqkldv
     1861 nsmlqeqaie evhmedseet qersehhned sqdsqddiqd vtgvskwerm eaedkldrld
     1921 glakkknegk vqtmkdflqv pdyynmedts gkkrvrwadl eerkeqekmr avgfvvghtn
     1981 wdrmmdptkg gsaltrtkff sls
//
LOCUS       XP_034187793             942 aa            linear   INV 03-SEP-2025
DEFINITION  secretory pathway calcium atpase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187793
VERSION     XP_034187793.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331902.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..942
                     /product="secretory pathway calcium atpase isoform X1"
                     /calculated_mol_wt=104030
     Region          77..918
                     /region_name="P-type_ATPase_SPCA"
                     /note="golgi-associated secretory pathway Ca(2+) transport
                     ATPases, similar to human ATPase secretory pathway Ca(2+)
                     transporting 1/hSPCA1 and Saccharomyces cerevisiae
                     Ca(2+)/Mn(2+)-transporting P-type ATPase, Pmr1p; cd02085"
                     /db_xref="CDD:319779"
     Site            order(371..373,475,501..503,546,591..593,640,643,646,665,
                     668)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319779"
     Site            order(726,730..731,733..734,759,762..763)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319779"
     CDS             1..942
                     /gene="SPoCk"
                     /coded_by="XM_034331902.2:390..3218"
                     /db_xref="GeneID:117607784"
ORIGIN      
        1 mkdtankkye qlfdeneldn aankecseda tlkedktptp emwlttaesa slgaeevaar
       61 lhvdvrtglw wqeadhrrql vgynefcvke eeptwkkyle qfknplilll lgsafvsvcm
      121 kqfddavsit vaiiivvtva fvqeyrseks leelnklvpp achclregrl etflarnlvp
      181 gdivylnigd rvpadirife aidlaidess ftgetepaqk stapllktng lttkrniafm
      241 gtlvrcgngk gvvvntgeks efgevfsmmq aeeapktplq rsmdilgtql slysfciigi
      301 imllgwiqgk ailemftigv slavaaipeg lpivvtvtla lgvmrmakrk vivkklptve
      361 tlgcvnvics dktgtitkne mtatilvtsd gyiaditgag yndkgevrir kcdnfdlart
      421 aisnmlevac vcnnaiiqgd illgqptega llaltlkygm ygvadkymrl qeypfsseqk
      481 mmavkcapky genrqevyfi kgavekilpl ctkyyvngev calsekkdee flaeaydigq
      541 qglrviglar gtslqdlayv glvgicdppr phvreaittl insgvkvkmv tgdaketasa
      601 iasmigldvl hsrlisgdei dlmsehqleq cisnvsvfyr vtpkhklciv kalqregniv
      661 gmtgdgvndg valkkadigi amgkngtdvc keaadmilvd ddfgtiiaai eegkgifhni
      721 rnfvrfqlst siaalslial atlmgipnpl namqilwini imdgppaqsl gvepvdkdvl
      781 kqkprntkep mitrhlivnv llsaiiiilg tlwvynremt tdgrhtardt tmtftcfvff
      841 dmfnalscrs qtksiftigl wsnkmflvav tlsvigqmlv iyfpplqrvf qtealwakdi
      901 lflvaltssv fiiseikkfv erqlyrrrag tqyysksemd fv
//
LOCUS       XP_034187801             931 aa            linear   INV 03-SEP-2025
DEFINITION  secretory pathway calcium atpase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187801
VERSION     XP_034187801.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331910.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..931
                     /product="secretory pathway calcium atpase isoform X2"
                     /calculated_mol_wt=102755
     Region          77..918
                     /region_name="P-type_ATPase_SPCA"
                     /note="golgi-associated secretory pathway Ca(2+) transport
                     ATPases, similar to human ATPase secretory pathway Ca(2+)
                     transporting 1/hSPCA1 and Saccharomyces cerevisiae
                     Ca(2+)/Mn(2+)-transporting P-type ATPase, Pmr1p; cd02085"
                     /db_xref="CDD:319779"
     Site            order(371..373,475,501..503,546,591..593,640,643,646,665,
                     668)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319779"
     Site            order(726,730..731,733..734,759,762..763)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319779"
     CDS             1..931
                     /gene="SPoCk"
                     /coded_by="XM_034331910.2:395..3190"
                     /db_xref="GeneID:117607784"
ORIGIN      
        1 mkdtankkye qlfdeneldn aankecseda tlkedktptp emwlttaesa slgaeevaar
       61 lhvdvrtglw wqeadhrrql vgynefcvke eeptwkkyle qfknplilll lgsafvsvcm
      121 kqfddavsit vaiiivvtva fvqeyrseks leelnklvpp achclregrl etflarnlvp
      181 gdivylnigd rvpadirife aidlaidess ftgetepaqk stapllktng lttkrniafm
      241 gtlvrcgngk gvvvntgeks efgevfsmmq aeeapktplq rsmdilgtql slysfciigi
      301 imllgwiqgk ailemftigv slavaaipeg lpivvtvtla lgvmrmakrk vivkklptve
      361 tlgcvnvics dktgtitkne mtatilvtsd gyiaditgag yndkgevrir kcdnfdlart
      421 aisnmlevac vcnnaiiqgd illgqptega llaltlkygm ygvadkymrl qeypfsseqk
      481 mmavkcapky genrqevyfi kgavekilpl ctkyyvngev calsekkdee flaeaydigq
      541 qglrviglar gtslqdlayv glvgicdppr phvreaittl insgvkvkmv tgdaketasa
      601 iasmigldvl hsrlisgdei dlmsehqleq cisnvsvfyr vtpkhklciv kalqregniv
      661 gmtgdgvndg valkkadigi amgkngtdvc keaadmilvd ddfgtiiaai eegkgifhni
      721 rnfvrfqlst siaalslial atlmgipnpl namqilwini imdgppaqsl gvepvdkdvl
      781 kqkprntkep mitrhlivnv llsaiiiilg tlwvynremt tdgrhtardt tmtftcfvff
      841 dmfnalscrs qtksiftigl wsnkmflvav tlsvigqmlv iyfpplqrvf qtealwakdi
      901 lflvaltssv fiiseikkfv erqlyrrrav y
//
LOCUS       XP_034187810             601 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-1,2-mannosyltransferase Alg9 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187810
VERSION     XP_034187810.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..601
                     /product="alpha-1,2-mannosyltransferase Alg9 isoform X1"
                     /calculated_mol_wt=69647
     Region          43..466
                     /region_name="Glyco_transf_22"
                     /note="Alg9-like mannosyltransferase family; pfam03901"
                     /db_xref="CDD:281842"
     Region          <499..>573
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     CDS             1..601
                     /gene="Alg9"
                     /coded_by="XM_034331919.2:55..1860"
                     /db_xref="GeneID:117607793"
ORIGIN      
        1 mapnqrqrql fiskkelkkl ngrrfskpee ssgtgliypg vdtafkllls arfcsaiwsh
       61 itdcdetyny wepshyllyg tgqqtweysp qyalrsytyl lihmvpakly hyllepnpvl
      121 vfyfvrclls vgcalsevyf yknvcrefgi higrltlvfl ilssgmyias aaflpssfsm
      181 ylstvataaw yarqyelaif ataisallgw pfaallgipi avemlvqkqe wgqfikwvii
      241 sgvvvlvpvv widsmyygkl viaplniity nvftnhgpni ygtepfsyyi yngflnfnfi
      301 flgalwapig lflvwlivpa rprdrlclsy wyslaplylw flvfffqphk eerflfpvyp
      361 miclagaiav dviqklyffi rtklsplhia yhylqytahi tffailicgl lgisrslaly
      421 kgyyapmevm idankfgleg eipkdmyinf cvgkewhrfp gsfffpsnnw klqflksefk
      481 gqlpqpfldh enatsviqpy fndmnreept ryfnvnkchf vldldidtet elepnysrlt
      541 dnftviksak flnsakshqf frafyvpfvs hkfctygsyn llqniklkti pllskdkhtk
      601 s
//
LOCUS       XP_034187819             498 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-1,2-mannosyltransferase Alg9 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187819
VERSION     XP_034187819.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331928.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="alpha-1,2-mannosyltransferase Alg9 isoform X2"
                     /calculated_mol_wt=57560
     Region          2..363
                     /region_name="PMT_2"
                     /note="Dolichyl-phosphate-mannose-protein
                     mannosyltransferase; cl21590"
                     /db_xref="CDD:473917"
     Region          <396..>470
                     /region_name="ALP_like"
                     /note="alkaline phosphatases and sulfatases; cl23718"
                     /db_xref="CDD:474031"
     CDS             1..498
                     /gene="Alg9"
                     /coded_by="XM_034331928.2:400..1896"
                     /db_xref="GeneID:117607793"
ORIGIN      
        1 mvpaklyhyl lepnpvlvfy fvrcllsvgc alsevyfykn vcrefgihig rltlvflils
       61 sgmyiasaaf lpssfsmyls tvataawyar qyelaifata isallgwpfa allgipiave
      121 mlvqkqewgq fikwviisgv vvlvpvvwid smyygklvia plniitynvf tnhgpniygt
      181 epfsyyiyng flnfnfiflg alwapiglfl vwlivparpr drlclsywys laplylwflv
      241 fffqphkeer flfpvypmic lagaiavdvi qklyffirtk lsplhiayhy lqytahitff
      301 ailicgllgi srslalykgy yapmevmida nkfglegeip kdmyinfcvg kewhrfpgsf
      361 ffpsnnwklq flksefkgql pqpfldhena tsviqpyfnd mnreeptryf nvnkchfvld
      421 ldidtetele pnysrltdnf tviksakfln sakshqffra fyvpfvshkf ctygsynllq
      481 niklktipll skdkhtks
//
LOCUS       XP_034187833             550 aa            linear   INV 03-SEP-2025
DEFINITION  ASCIZ zinc finger protein [Osmia lignaria lignaria].
ACCESSION   XP_034187833
VERSION     XP_034187833.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331942.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187833.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..550
                     /product="ASCIZ zinc finger protein"
                     /calculated_mol_wt=62332
     Region          33..58
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(38,40,47,49..50,53..54,57,81,86,90..91,94..95,99,
                     112,114,116,118..119,122..123,126)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          71..100
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          107..126
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..550
                     /gene="Asciz"
                     /coded_by="XM_034331942.2:101..1753"
                     /db_xref="GeneID:117607803"
ORIGIN      
        1 meklkpvknv skgvkiicps peelsvitnn ikcekcglvf knepryrlhd lkvhqrknld
       61 ktikenvqyh cpvesciyap kaerhfnsmk ylkqhylkvh akkthacnlc dksfsteaak
      121 eghmrvcgie ftcscsktyt syeallthak rslhtvnnky knslrrassk tirlilptsq
      181 teinklitil pinqnenkis nhsngttqkl tsdigvqtde hrrnkrilsp skhtnnshch
      241 nekreiskqt qtnisqkirq nvmsmetqtt aapqtfkntl riqkhrknsv sqnsdytllk
      301 edlnlgnftp snlfpssplp lrhddglqdf wedkntsgtq tipekdmfev lndnvtqtef
      361 etfydhstnp liqcvpkstv almtlpyvee tssvgggysf vssnsisrsd pmltdktfnd
      421 rfssietqte qaysqsffds dtltrsftls stietqttnn ldnmellysn tctqtcneil
      481 psdlglsniq tqtawtqled ttvstetqtk slicetgcni pisacrswls tqishtetqt
      541 dllsifeglq
//
LOCUS       XP_034187838             501 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (3) 04053 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034187838
VERSION     XP_034187838.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331947.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="lethal (3) 04053 isoform X1"
                     /calculated_mol_wt=55901
     Region          321..>485
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     CDS             1..501
                     /gene="l(3)04053"
                     /coded_by="XM_034331947.2:52..1557"
                     /db_xref="GeneID:117607813"
ORIGIN      
        1 mmanrkksne lsrpvsrnvr rhvgevmfps sssseeeitt gcrgcrnmsk eqfvknrter
       61 gsqagqdasr seenpfsfkh flkngsrsny hnvgarpkiy ssptpspfnl ekdstvyprn
      121 ptelpdfvqd hlvieqyyln qeqkqqtisd vdnlpdfaln sveqrqnrlr neskksessa
      181 scdlpfdltg sidkglsnrn qsvpnissah lnlpafvkss vdsiehpess gscrmsyyii
      241 sttinfilil dhhlgfpldl pipvseshpg snitsrdcsg tneanipksl pdflndgpih
      301 nrttlsgesg sipnsvepte rrlllenerl ryelelaqkq isekseriql leseltsrrt
      361 veheetvhle kameqvednl krstrravna estvsslkke ikslttelsl lrlentelka
      421 gltagnrnec gssnvdrtvk rlasdlrsaa ssaevslrql msgvdnlrvl asalenvdri
      481 edrtkdflpd fdednaagpa l
//
LOCUS       XP_034187846             478 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (3) 04053 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034187846
VERSION     XP_034187846.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331955.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="lethal (3) 04053 isoform X2"
                     /calculated_mol_wt=53264
     Region          197..>453
                     /region_name="PLN02939"
                     /note="transferase, transferring glycosyl groups"
                     /db_xref="CDD:215507"
     CDS             1..478
                     /gene="l(3)04053"
                     /coded_by="XM_034331955.2:49..1485"
                     /db_xref="GeneID:117607813"
ORIGIN      
        1 mmanrkksne lsrpvsrnvr rhvgevmfps sssseeeitt gcrgcrnmsk eqfvknrter
       61 gsqagqdasr seenpfsfkh flkngsrsny hnvgarpkiy ssptpspfnl ekdstvyprn
      121 ptelpdfvqd hlvieqyyln qeqkqqtisd vdnlpdfaln sveqrqnrlr neskksessa
      181 scdlpfdltg sidkglsnrn qsvpnissah lnlpafvkss vdsiehpess grfpldlpip
      241 vseshpgsni tsrdcsgtne anipkslpdf lndgpihnrt tlsgesgsip nsvepterrl
      301 llenerlrye lelaqkqise kseriqlles eltsrrtveh eetvhlekam eqvednlkrs
      361 trravnaest vsslkkeiks lttelsllrl entelkaglt agnrnecgss nvdrtvkrla
      421 sdlrsaassa evslrqlmsg vdnlrvlasa lenvdriedr tkdflpdfde dnaagpal
//
LOCUS       XP_034187857             477 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (3) 04053 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034187857
VERSION     XP_034187857.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331966.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="lethal (3) 04053 isoform X3"
                     /calculated_mol_wt=53108
     Region          197..>452
                     /region_name="PLN02939"
                     /note="transferase, transferring glycosyl groups"
                     /db_xref="CDD:215507"
     CDS             1..477
                     /gene="l(3)04053"
                     /coded_by="XM_034331966.2:49..1482"
                     /db_xref="GeneID:117607813"
ORIGIN      
        1 mmanrkksne lsrpvsrnvr rhvgevmfps sssseeeitt gcrgcrnmsk eqfvknrter
       61 gsqagqdasr seenpfsfkh flkngsrsny hnvgarpkiy ssptpspfnl ekdstvyprn
      121 ptelpdfvqd hlvieqyyln qeqkqqtisd vdnlpdfaln sveqrqnrlr neskksessa
      181 scdlpfdltg sidkglsnrn qsvpnissah lnlpafvkss vdsiehpess gfpldlpipv
      241 seshpgsnit srdcsgtnea nipkslpdfl ndgpihnrtt lsgesgsipn svepterrll
      301 lenerlryel elaqkqisek seriqllese ltsrrtvehe etvhlekame qvednlkrst
      361 rravnaestv sslkkeiksl ttelsllrle ntelkaglta gnrnecgssn vdrtvkrlas
      421 dlrsaassae vslrqlmsgv dnlrvlasal envdriedrt kdflpdfded naagpal
//
LOCUS       XP_034187872             474 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,3-glucan recognition protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034187872
VERSION     XP_034187872.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331981.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="beta-1,3-glucan recognition protein 1"
                     /calculated_mol_wt=53890
     Region          29..133
                     /region_name="CBM39"
                     /note="Carbohydrate binding domain (family 32); pfam15886"
                     /db_xref="CDD:464924"
     Region          171..473
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     Site            order(295,297,299,315,317,320,337,339,364,413,415)
                     /site_type="active"
                     /db_xref="CDD:185683"
     Site            order(315,317,320)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:185683"
     CDS             1..474
                     /gene="B-gluc1"
                     /coded_by="XM_034331981.2:53..1477"
                     /db_xref="GeneID:117607827"
ORIGIN      
        1 mtalptkmll legflllivv qenfaqylpp tptveplypk glrmsiphed gislvayhvk
       61 fnedfnglea gtiardivka rngrwtyedr ttrlkrgqki yywihvvydg lgynlvdqqh
      121 evnefynydg tpvsastggn ivcarpsetr tfetdrntpq tnrqnicpgq llfednfdtl
      181 dssrwktier fshapnyefv vykndrnnvd vwdgvlhirp tltnvvfgrd firegtltle
      241 kctseigtan ckqvgrapfi lppviagrin skpsflflyg hvqiraklpr gdwlyplitl
      301 estegntdds kayceiiiah svgnpslqlk dgkdfsghll qaggyavskd nqnkqnnwfn
      361 llsknssslw sdsyhvfdli wkpgqvvvkv dneqygeqql pplydtplyl nfgiavgghv
      421 vfhntctsgh yekpwknlsp kalndfyqae snwletwhns dtglhidyvk vwai
//
LOCUS       XP_034187898              99 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607837 [Osmia lignaria lignaria].
ACCESSION   XP_034187898
VERSION     XP_034187898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332007.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..99
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..99
                     /product="uncharacterized protein LOC117607837"
                     /calculated_mol_wt=11145
     CDS             1..99
                     /gene="LOC117607837"
                     /coded_by="XM_034332007.2:110..409"
                     /db_xref="GeneID:117607837"
ORIGIN      
        1 mnfprnlvfl tcivllfhpd ilpvsaaepi svfsfihdli qynvagipii hertewdfdp
       61 evgkqrrvky eqengrhgei aiekigmgig ykgpwgtpt
//
LOCUS       XP_034187913              82 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607843 [Osmia lignaria lignaria].
ACCESSION   XP_034187913
VERSION     XP_034187913.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332022.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187913.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..82
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..82
                     /product="uncharacterized protein LOC117607843"
                     /calculated_mol_wt=9311
     CDS             1..82
                     /gene="LOC117607843"
                     /coded_by="XM_034332022.2:380..628"
                     /db_xref="GeneID:117607843"
ORIGIN      
        1 marhgfwtti ilatiivafl lglitenaat iaqpkapnfq yferpkyryp yydengrgkl
       61 lygyggpdly qyktysaleg ih
//
LOCUS       XP_034187925            1754 aa            linear   INV 03-SEP-2025
DEFINITION  murinoglobulin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034187925
VERSION     XP_034187925.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187925.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1754
                     /product="murinoglobulin-1"
                     /calculated_mol_wt=197701
     Region          132..225
                     /region_name="MG2"
                     /note="MG2 domain; pfam01835"
                     /db_xref="CDD:426464"
     Region          227..314
                     /region_name="MG3"
                     /note="Macroglobulin domain MG3; pfam17791"
                     /db_xref="CDD:465509"
     Region          358..457
                     /region_name="MG4"
                     /note="Macroglobulin domain MG4; pfam17789"
                     /db_xref="CDD:465507"
     Region          486..647
                     /region_name="A2M_BRD"
                     /note="Alpha-2-macroglobulin bait region domain;
                     pfam07703"
                     /db_xref="CDD:462235"
     Region          802..892
                     /region_name="A2M"
                     /note="Alpha-2-macroglobulin family; pfam00207"
                     /db_xref="CDD:459711"
     Region          1020..1305
                     /region_name="ISOPREN_C2_like"
                     /note="This group contains class II terpene cyclases,
                     protein prenyltransferases beta subunit, two broadly
                     specific proteinase inhibitors alpha2-macroglobulin (alpha
                     (2)-M) and pregnancy zone protein (PZP) and, the C3 C4 and
                     C5 components of vertebrate...; cl08267"
                     /db_xref="CDD:415487"
     Region          1411..1501
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1754
                     /gene="LOC117607849"
                     /coded_by="XM_034332034.2:188..5452"
                     /db_xref="GeneID:117607849"
ORIGIN      
        1 mdkllflvtl cclvgfircn ltssniergy vftipnrlla getvsgclsl hnleppahvl
       61 lellsqpseq gvlsstdtvl ttgietclel miptpqdttv flrltikfek hpeyvvnvmk
      121 mmeiqqnsli tfvetdkpiy kpgqdvniri lmlkhdlkpw kkpiskvwie tpaevrvaqw
      181 snvstengmv qlafplssep rwgmwkikve kysnpqliht ttfevtkyvl prfqisitsp
      241 kyvladaenv twnicvkysy nkpvkgtlll kltpdirhwr itynvteiry ktemdspdgc
      301 tkfaisgsil nlprwsvdpe niilianfte agtgvvetmi nwtpvkheal kiefaqympk
      361 yfkldlpyhg klrvlrqdnt paptekiqfc lkvrrkhrly hdvvecrdft ssadgfidfi
      421 vppqhknvil lsfvatavdy smkyyapenr wkvfmnqpsa hinvnpwysp sdsylavarg
      481 sqpivcgeky sfnvmytipp dtevnesilf hysinskgdi liyghvkhkp grdtilnyse
      541 frnllgsles senktdqdpi vhrfplsvkv tssmapisel llyyvrsdge ivaatytiev
      601 ghcfenkvkt awhtdvqtpg sttqyhveaa pwslcaisai destlflsgl ksnlinaaqt
      661 ferlkmyhpt sdlrenwmqt ncemsapnee fahlplpavq rglgwlrkrr sntyhrsvdy
      721 vnamqafndf gvvvmsnlvq karpcsvgfs eqlyvsnsmr mmtmdrtvid savaaaamdp
      781 gveyvdesfd qtptlrsyfp etwlwelvpt gkegkvtier tlpdtitdwv sytacispih
      841 glgiapptti tafqsffldy slpysvkrge mfrmkvslfn ymqhslpvki klenisgvdl
      901 hlshptasfc vkprdsvvhe yvlrprvige vnitvsasvd tdyfdpcgpq tlvftrdtiv
      961 kpililpegf pveetktmlv cptdfnddst fmwevrfped vvpdsgrayv tlvgdvlgpa
     1021 lenldklvrv ptgcgeqtml lfvpnfhvlr yldvleikvp alreraiknm eegyqkeltf
     1081 rkpdgsyssf stsassiwlt afvlksfgqa rsyihidepd lemtaywivr kqlengcfpi
     1141 igtvfhkqmk galqddgssa altayivisl lksrvplpas lisnsfrcle kimdnsnddl
     1201 yttilttyav alsehpfana svqtlmdlat ryngliwwed ktrpsmslsi emtsyviltl
     1261 vklggtenll ealkavrwls kqrnakggfi stqdtilgle alaeyamtis tkntdlsvlv
     1321 takeidvvhr lynenrmllk qihlpvlpti vevfaegkgc ilvqtniryn vphttgsdaf
     1381 dltvaassda yanecsiqdi ticaryklad gesnmallei gmisgyvpdr eslqsllqpt
     1441 srvkrfeedn dmvsiyfdkl tsqkncisfk iirenvvdrl epanvklydy yqqelmvsss
     1501 ykiasmcgsa epidekpakn ltfqlsairl ptkdvlnssf vvmkheldvp egmegpvpvy
     1561 vkpntidykm ettiatngvp eltptlgted qtvqwgeqep vqeefdsqik nttdietdvw
     1621 sptgaeqfcp icmdkipsdi rniycsassv vkvairrlrk armlldlhas revqrlratv
     1681 eftlssncsc spldtpgsla liinkdndfl asgdhkqtln dsfyiyglpp isgvpctvae
     1741 arstcsneek vqct
//
LOCUS       XP_034187937             904 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607857 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187937
VERSION     XP_034187937.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332046.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..904
                     /product="uncharacterized protein LOC117607857 isoform X1"
                     /calculated_mol_wt=98156
     Region          7..253
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(18,21..22,26,28..29,32..33,35..36,47..48,50..51,
                     54..55,57..58,61,86,89..90,201,204..205,207..208,211..212,
                     214..215,218..219,222..223,225..226,228..229,231..232)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          875..901
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(875,877..879,881..883,888..891)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..904
                     /gene="LOC117607857"
                     /coded_by="XM_034332046.2:601..3315"
                     /db_xref="GeneID:117607857"
ORIGIN      
        1 mdatierecs algglfqqii tdmkngaplw edliskatkl hsclraaila vsayletfqk
       61 iadaatnarg atkeigtalt riclrhkave trmksftsai mdclvlplqe kledwkksli
      121 nldkehakey kkakaelrkr stdtlrlqkk karksqhlqg qtqchgplsg dielnrmles
      181 saavvqekrl sleeterrav raalleergr ycllarflkp vldeeiamlm elthlqevsd
      241 qlqrhaasph hlppaseqvi tdikgcdvtq wslatppssp slslgsrkss mcsissltss
      301 ssgsckshps psghpwhrsl sqpagvrpss isgmmtlrhl angssrdsgf tsqdtlytqp
      361 isehqvsnvs sqtnkntgct ttrpvttatw pdlqetsvqy drqnqntvne rphtissaye
      421 kghqrpalsv ytfqapdvsg cchsqpaspv ssssscsssn qasrvqlrrn ngsnrppipn
      481 rcsslerptv pmkneppgsp rgkpklplpa hlakelathq lqqpmyvnmh elanlaasra
      541 qemqlplppp paslstpgte ktevtekdgg sqtsessves ssgygsqtti phshslqnqn
      601 ennwnvpgaa stlmirrgsm qqaikppppt rrtstiitat fnnpsstsnd ertdntcdev
      661 enlppppafl legssptasp tpqrsisvse tvrtltelrh tpaspsllrk atgqtqshnn
      721 tsttlqhnav lqvtrtnver scaairsasq ergsntttqv tgistsvniq gqgpggvgqs
      781 fmavlsakli nstgnnnttt gnntsnspks arkhsveqqi snkntktpsg fletlnakla
      841 qqqqtlqgnn ttsrsasvrr imgnrvpimd plqvrdslmd qirkgtslrk tsgpindrsa
      901 pkiy
//
LOCUS       XP_034187945             875 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607857 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187945
VERSION     XP_034187945.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332054.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..875
                     /product="uncharacterized protein LOC117607857 isoform X2"
                     /calculated_mol_wt=95065
     Region          7..253
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(18,21..22,26,28..29,32..33,35..36,47..48,50..51,
                     54..55,57..58,61,86,89..90,201,204..205,207..208,211..212,
                     214..215,218..219,222..223,225..226,228..229,231..232)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          846..872
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(846,848..850,852..854,859..862)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..875
                     /gene="LOC117607857"
                     /coded_by="XM_034332054.2:602..3229"
                     /db_xref="GeneID:117607857"
ORIGIN      
        1 mdatierecs algglfqqii tdmkngaplw edliskatkl hsclraaila vsayletfqk
       61 iadaatnarg atkeigtalt riclrhkave trmksftsai mdclvlplqe kledwkksli
      121 nldkehakey kkakaelrkr stdtlrlqkk karksqhlqg qtqchgplsg dielnrmles
      181 saavvqekrl sleeterrav raalleergr ycllarflkp vldeeiamlm elthlqevsd
      241 qlqrhaasph hlppaseqvi tdikgcdvtq wslatppssp slslgsrkss mcsissltss
      301 ssgsckshps psghpwhrsl sqpagvrpss isgmmtlrhl angssrdsgf tsqdtlytqp
      361 isehqetsvq ydrqnqntvn erphtissay ekghqrpals vytfqapdvs gcchsqpasp
      421 vssssscsss nqasrvqlrr nngsnrppip nrcsslerpt vpmkneppgs prgkpklplp
      481 ahlakelath qlqqpmyvnm helanlaasr aqemqlplpp ppaslstpgt ektevtekdg
      541 gsqtsessve sssgygsqtt iphshslqnq nennwnvpga astlmirrgs mqqaikpppp
      601 trrtstiita tfnnpsstsn dertdntcde venlppppaf llegssptas ptpqrsisvs
      661 etvrtltelr htpaspsllr katgqtqshn ntsttlqhna vlqvtrtnve rscaairsas
      721 qergsntttq vtgistsvni qgqgpggvgq sfmavlsakl instgnnntt tgnntsnspk
      781 sarkhsveqq isnkntktps gfletlnakl aqqqqtlqgn nttsrsasvr rimgnrvpim
      841 dplqvrdslm dqirkgtslr ktsgpindrs apkiy
//
LOCUS       XP_034187955             861 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607857 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187955
VERSION     XP_034187955.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332064.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="uncharacterized protein LOC117607857 isoform X3"
                     /calculated_mol_wt=93554
     Region          7..253
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(18,21..22,26,28..29,32..33,35..36,47..48,50..51,
                     54..55,57..58,61,86,89..90,201,204..205,207..208,211..212,
                     214..215,218..219,222..223,225..226,228..229,231..232)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          832..858
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(832,834..836,838..840,845..848)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..861
                     /gene="LOC117607857"
                     /coded_by="XM_034332064.2:601..3186"
                     /db_xref="GeneID:117607857"
ORIGIN      
        1 mdatierecs algglfqqii tdmkngaplw edliskatkl hsclraaila vsayletfqk
       61 iadaatnarg atkeigtalt riclrhkave trmksftsai mdclvlplqe kledwkksli
      121 nldkehakey kkakaelrkr stdtlrlqkk karksqhlqg qtqchgplsg dielnrmles
      181 saavvqekrl sleeterrav raalleergr ycllarflkp vldeeiamlm elthlqevsd
      241 qlqrhaasph hlppaseqvi tdikgcdvtq wslatppssp slslgsrkss mcsissltss
      301 ssgsckshps psghpwhrsl sqvsnvssqt nkntgctttr pvttatwpdl qetsvqydrq
      361 nqntvnerph tissayekgh qrpalsvytf qapdvsgcch sqpaspvsss sscsssnqas
      421 rvqlrrnngs nrppipnrcs slerptvpmk neppgsprgk pklplpahla kelathqlqq
      481 pmyvnmhela nlaasraqem qlplppppas lstpgtekte vtekdggsqt sessvesssg
      541 ygsqttiphs hslqnqnenn wnvpgaastl mirrgsmqqa ikpppptrrt stiitatfnn
      601 psstsndert dntcdevenl ppppaflleg ssptasptpq rsisvsetvr tltelrhtpa
      661 spsllrkatg qtqshnntst tlqhnavlqv trtnversca airsasqerg sntttqvtgi
      721 stsvniqgqg pggvgqsfma vlsaklinst gnnntttgnn tsnspksark hsveqqisnk
      781 ntktpsgfle tlnaklaqqq qtlqgnntts rsasvrrimg nrvpimdplq vrdslmdqir
      841 kgtslrktsg pindrsapki y
//
LOCUS       XP_034187965             832 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607857 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187965
VERSION     XP_034187965.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332074.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..832
                     /product="uncharacterized protein LOC117607857 isoform X4"
                     /calculated_mol_wt=90464
     Region          7..253
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(18,21..22,26,28..29,32..33,35..36,47..48,50..51,
                     54..55,57..58,61,86,89..90,201,204..205,207..208,211..212,
                     214..215,218..219,222..223,225..226,228..229,231..232)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          803..829
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(803,805..807,809..811,816..819)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     CDS             1..832
                     /gene="LOC117607857"
                     /coded_by="XM_034332074.2:602..3100"
                     /db_xref="GeneID:117607857"
ORIGIN      
        1 mdatierecs algglfqqii tdmkngaplw edliskatkl hsclraaila vsayletfqk
       61 iadaatnarg atkeigtalt riclrhkave trmksftsai mdclvlplqe kledwkksli
      121 nldkehakey kkakaelrkr stdtlrlqkk karksqhlqg qtqchgplsg dielnrmles
      181 saavvqekrl sleeterrav raalleergr ycllarflkp vldeeiamlm elthlqevsd
      241 qlqrhaasph hlppaseqvi tdikgcdvtq wslatppssp slslgsrkss mcsissltss
      301 ssgsckshps psghpwhrsl sqetsvqydr qnqntvnerp htissayekg hqrpalsvyt
      361 fqapdvsgcc hsqpaspvss ssscsssnqa srvqlrrnng snrppipnrc sslerptvpm
      421 kneppgsprg kpklplpahl akelathqlq qpmyvnmhel anlaasraqe mqlplppppa
      481 slstpgtekt evtekdggsq tsessvesss gygsqttiph shslqnqnen nwnvpgaast
      541 lmirrgsmqq aikpppptrr tstiitatfn npsstsnder tdntcdeven lppppaflle
      601 gssptasptp qrsisvsetv rtltelrhtp aspsllrkat gqtqshnnts ttlqhnavlq
      661 vtrtnversc aairsasqer gsntttqvtg istsvniqgq gpggvgqsfm avlsaklins
      721 tgnnntttgn ntsnspksar khsveqqisn kntktpsgfl etlnaklaqq qqtlqgnntt
      781 srsasvrrim gnrvpimdpl qvrdslmdqi rkgtslrkts gpindrsapk iy
//
LOCUS       XP_034187978             529 aa            linear   INV 03-SEP-2025
DEFINITION  bifunctional Phosphopantetheine adenylyltransferase - Dephospho-CoA
            kinase [Osmia lignaria lignaria].
ACCESSION   XP_034187978
VERSION     XP_034187978.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332087.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187978.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..529
                     /product="bifunctional Phosphopantetheine
                     adenylyltransferase - Dephospho-CoA kinase"
                     /calculated_mol_wt=59895
     Region          161..307
                     /region_name="PPAT_CoAS"
                     /note="phosphopantetheine adenylyltransferase domain of
                     eukaryotic and archaeal bifunctional enzymes; cd02164"
                     /db_xref="CDD:173915"
     Site            order(166..167,195,255)
                     /site_type="active"
                     /db_xref="CDD:173915"
     Site            172..175
                     /site_type="other"
                     /note="(T/H)XGH motif"
                     /db_xref="CDD:173915"
     Region          325..519
                     /region_name="CoaE"
                     /note="Dephospho-CoA kinase [Coenzyme transport and
                     metabolism]; COG0237"
                     /db_xref="CDD:440007"
     CDS             1..529
                     /gene="Ppat-Dpck"
                     /coded_by="XM_034332087.2:182..1771"
                     /db_xref="GeneID:117607876"
ORIGIN      
        1 mantgllvlt nptkitkllp iikehvlktl yiqylpekni flsrnhaftn sqwkvlssca
       61 qtiskiytdt stifppldir vlltsiknpn lsiintkkpv eivifdqvys tkeaytfiqd
      121 ylantsmscn fvtfnddqev ekisntcink dgvneekiyk nvvlggtfdr ihnghkiflt
      181 eavlrcsekl tvgvtdtnml sgkllwelie pcqtrilnlk dfledidstl mydvvpindm
      241 ygptksdpsf emivvseetk rggdkvneir vknnlnkldi hiiklisden hrnheeskvs
      301 ssnhrirllg trlrppkien ksvkpyiigl tggiasgkss vadkleklga glvncdkiah
      361 dlylpgkkcf daivenfgst ilkpdgfidr kvlgnivfnn keqlnklnni vwpiileeak
      421 kqindfyakg fdvivmeaav liqanwqhfc heiwtciipq eeairrvier ngltvddakl
      481 riqaqpsnve qvneanvvic tlwshditle qvqkawdelm kllstqtkn
//
LOCUS       XP_034187992             496 aa            linear   INV 03-SEP-2025
DEFINITION  double-stranded RNA-specific editase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034187992
VERSION     XP_034187992.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332101.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="double-stranded RNA-specific editase 1"
                     /calculated_mol_wt=54600
     Region          238..300
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Site            order(245..246,248,267,284..286,289)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380694"
     Region          417..>458
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     CDS             1..496
                     /gene="LOC117607880"
                     /coded_by="XM_034332101.2:197..1687"
                     /db_xref="GeneID:117607880"
ORIGIN      
        1 mytrnrqsqg yanarnqnnp nigyqgqnty nqqggfqgtp panpssnfkq gsvqnvqafk
       61 qqpppqmqqa vsrnlqqvkp pqlqqqpspq ikppqqpian spqiqpqpqs qpppqsqsql
      121 qppsqpqppp qrlkpilkqp sqpvqqqssi ttqnnpvsss qpsvptpasp lttnsnptpp
      181 vnnnsselkt enqvpentdv emqeqlprwr rkapgfrvsk klkrlrlnqr lrktlqpkna
      241 imvlnemkag vqftfpetqg pmpnslylvh aeldgktyvg qglskplarq naaenalkal
      301 llekmtaasl karidaesdg qsnqstdsfn tskddntedg gtsmdttide sneipwssla
      361 sfalyklfle whnqgtsvpv prpglpspvk gikreisqvp ktpvqkelpp natnihpvml
      421 lnqmrpglty ielnrasnpp ntmftlavdi dgveytgtak nkkdakkiaa ksallalygl
      481 sypdetipip qeqqma
//
LOCUS       XP_076546389             298 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117607887 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546389
VERSION     XP_076546389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690274.1
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117607887"
                     /calculated_mol_wt=34708
     Region          <170..281
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          177..208
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          210..241
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..298
                     /gene="LOC117607887"
                     /coded_by="XM_076690274.1:1..897"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon"
                     /db_xref="GeneID:117607887"
ORIGIN      
        1 mgkclpyvds ylqllfldqe ishfksgfkk rkftyftsee enhlretktm sliikynesf
       61 ydvlwkyfme laeitfvifk ligavgfivv isyilyhfhe rrhtkfases keyfeklqvi
      121 knfnrsftrc qsiishkemf naleyaadkc snkayalkci divvccpqll nirssvnglt
      181 pfhricfqgh tyliafmlak gadpflstit genalcmavy hflndptknd fscleillqt
      241 gcefglknkw ydillkmafn snhiklaqwl ighyktslnk tlrcsstppv tkhykfsm
//
LOCUS       XP_034188017             192 aa            linear   INV 03-SEP-2025
DEFINITION  intraflagellar transport protein 22 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034188017
VERSION     XP_034188017.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332126.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188017.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..192
                     /product="intraflagellar transport protein 22 homolog"
                     /calculated_mol_wt=21946
     Region          5..>89
                     /region_name="P-loop_NTPase"
                     /note="P-loop containing Nucleoside Triphosphate
                     Hydrolases; cl38936"
                     /db_xref="CDD:476819"
     Site            10..17
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206735"
     Site            36
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206735"
     Site            43..45
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206735"
     Site            64..67
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206735"
     Site            order(66..67,83..84)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206735"
     CDS             1..192
                     /gene="LOC117607893"
                     /coded_by="XM_034332126.2:60..638"
                     /db_xref="GeneID:117607893"
ORIGIN      
        1 mrplkiivig pvrsgktmma nfladateip ydyyptqgvr ilefevndin vdnkyitkdi
       61 elwdcsgdpk fencwpairk dlhgvifvys eksndclkvi qqlydyfidq iklgpdrcvi
      121 fcydpekknp eiskiisstf vkvshvkcni esggsklkad fssfistilg kmhnyneqed
      181 knilsnnilf ar
//
LOCUS       XP_034183603            1206 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation regulator 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183603
VERSION     XP_034183603.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327712.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1206
                     /product="transcription elongation regulator 1 isoform X1"
                     /calculated_mol_wt=137484
     Region          196..221
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          305..385
                     /region_name="DUF1720"
                     /note="Domain of unknown function (DUF1720); pfam08226"
                     /db_xref="CDD:369766"
     Region          420..>874
                     /region_name="PRP40"
                     /note="Splicing factor [RNA processing and modification];
                     COG5104"
                     /db_xref="CDD:227435"
     Region          665..712
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          730..779
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          799..847
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          <880..>1189
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1206
                     /gene="LOC117605863"
                     /coded_by="XM_034327712.2:135..3755"
                     /db_xref="GeneID:117605863"
ORIGIN      
        1 metsteqtpv stetvpnneq ehneegmeet edysfdgedy rhfvprgrgg frgrgrggfd
       61 fpmrggpprf rgrgfgprgp mfrganngfp fegpprpncp pgpagprfrg pppfdpswgp
      121 mgppnlmgpp nlmgppgmpp phmmngpigt gpgpygpppg mgppnmnnip gqqqpqqqqa
      181 qqqanipgld lngevwvetk tpdgksyyyn irtrettwtk pegpnvkvmv qdqleqlvhg
      241 askqtvpsst ptstattpsq isenrvsqss eqppannada inqlstappg tgppptlget
      301 pnvnvqptds tqangtapat vtntptmntp avntnmmqpp pnmipmqhri pnqfggpiat
      361 qfgaapfgmp ppgfqpfggy gppqanwgip qmphgvmtpq apaedpaila qldqelvasa
      421 mvwtehrapd grlyyynska gesvwekpqa lkdlenakla lrqkaeeaav nstntavtss
      481 avpnnnvtte pikqdkpqes nhetkdsake tdtnkpkkee vapkeaakpq dksrpisstp
      541 vpgtpwcvvw tgdgrvffyn pssrisvwer pddligrqdv dkmvstppda vvatktprqs
      601 dtsessdddq ptpakkmkqe dtksvtpkee eekeskktid igkeaaieae vraareraiv
      661 pletriksfr dmlaekevsa fstwekelhk ivfdprylll tskerkqvfe kyvkeraeee
      721 rrekrnkmke rkeqfqklle eaglhgkssf sdfaqkhgrd erfknvekmr ereslfneyl
      781 levrkkekee ktakreqvkk effamlrehk didrhshwsd ckkklesdwr yrvvesastr
      841 edwfrdyirm lkeerkkeke kdkdhrhrdk dhhksdkkdr drkdvdkyke ksskdrvekd
      901 sskdkkqrrs eapseengke kkesvvekes geiedndekp skkendkeda edqsdseedr
      961 ekqkrererr aeaslrerer evqrtlathl rdrdkerqhh rhteavqhfs alladlvrng
     1021 dlawreakrq lrkdhrwela esldreeker lfnehieqls rkkrdkfrel ldevgastel
     1081 taswrdikkl lkddprylkf sssdrkceke fkeyikdklv aakadfrell qetklitdkt
     1141 ykkvqensah lveieeilrk drrflvleaa aaertrllmg yleelarrgp pppptaseps
     1201 rrpttn
//
LOCUS       XP_034183694            1205 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation regulator 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183694
VERSION     XP_034183694.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327803.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1205
                     /product="transcription elongation regulator 1 isoform X2"
                     /calculated_mol_wt=137356
     Region          196..221
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          305..385
                     /region_name="DUF1720"
                     /note="Domain of unknown function (DUF1720); pfam08226"
                     /db_xref="CDD:369766"
     Region          420..>874
                     /region_name="PRP40"
                     /note="Splicing factor [RNA processing and modification];
                     COG5104"
                     /db_xref="CDD:227435"
     Region          665..712
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          730..779
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          799..847
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          861..>981
                     /region_name="SF-CC1"
                     /note="splicing factor, CC1-like family; TIGR01622"
                     /db_xref="CDD:273721"
     Region          1003..1054
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          1060..1116
                     /region_name="FF"
                     /note="Contains two conserved F residues; smart00441"
                     /db_xref="CDD:128718"
     CDS             1..1205
                     /gene="LOC117605863"
                     /coded_by="XM_034327803.2:137..3754"
                     /db_xref="GeneID:117605863"
ORIGIN      
        1 metsteqtpv stetvpnneq ehneegmeet edysfdgedy rhfvprgrgg frgrgrggfd
       61 fpmrggpprf rgrgfgprgp mfrganngfp fegpprpncp pgpagprfrg pppfdpswgp
      121 mgppnlmgpp nlmgppgmpp phmmngpigt gpgpygpppg mgppnmnnip gqqqpqqqqa
      181 qqqanipgld lngevwvetk tpdgksyyyn irtrettwtk pegpnvkvmv qdqleqlvhg
      241 askqtvpsst ptstattpsq isenrvsqss eqppannada inqlstappg tgppptlget
      301 pnvnvqptds tqangtapat vtntptmntp avntnmmqpp pnmipmqhri pnqfggpiat
      361 qfgaapfgmp ppgfqpfggy gppqanwgip qmphgvmtpq apaedpaila qldqelvasa
      421 mvwtehrapd grlyyynska gesvwekpqa lkdlenakla lrqkaeeaav nstntavtss
      481 avpnnnvtte pikqdkpqes nhetkdsake tdtnkpkkee vapkeaakpq dksrpisstp
      541 vpgtpwcvvw tgdgrvffyn pssrisvwer pddligrqdv dkmvstppda vvatktprqs
      601 dtsessdddq ptpakkmkqe dtksvtpkee eekeskktid igkeaaieae vraareraiv
      661 pletriksfr dmlaekevsa fstwekelhk ivfdprylll tskerkqvfe kyvkeraeee
      721 rrekrnkmke rkeqfqklle eaglhgkssf sdfaqkhgrd erfknvekmr ereslfneyl
      781 levrkkekee ktakreqvkk effamlrehk didrhshwsd ckkklesdwr yrvvesastr
      841 edwfrdyirm lkeerkkeke kdkdhrhrdk dhhksdkkdr drkdvdkyke ksskdrvekd
      901 sskdkkqrrs eapseengke kkesvvekes geiedndekp skkendedae dqsdseedre
      961 kqkrererra easlrerere vqrtlathlr drdkerqhhr hteavqhfsa lladlvrngd
     1021 lawreakrql rkdhrwelae sldreekerl fnehieqlsr kkrdkfrell devgastelt
     1081 aswrdikkll kddprylkfs ssdrkcekef keyikdklva akadfrellq etklitdkty
     1141 kkvqensahl veieeilrkd rrflvleaaa aertrllmgy leelarrgpp ppptasepsr
     1201 rpttn
//
LOCUS       XP_034183781            1202 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation regulator 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183781
VERSION     XP_034183781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1202
                     /product="transcription elongation regulator 1 isoform X3"
                     /calculated_mol_wt=137058
     Region          192..217
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          301..381
                     /region_name="DUF1720"
                     /note="Domain of unknown function (DUF1720); pfam08226"
                     /db_xref="CDD:369766"
     Region          416..>870
                     /region_name="PRP40"
                     /note="Splicing factor [RNA processing and modification];
                     COG5104"
                     /db_xref="CDD:227435"
     Region          661..708
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          726..775
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          795..843
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          <876..>1185
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1202
                     /gene="LOC117605863"
                     /coded_by="XM_034327890.2:135..3743"
                     /db_xref="GeneID:117605863"
ORIGIN      
        1 metsteqtpv stetvpnneq ehneegmeet edysfdgedy rhfvprgrgg frggfdfpmr
       61 ggpprfrgrg fgprgpmfrg anngfpfegp prpncppgpa gprfrgpppf dpswgpmgpp
      121 nlmgppnlmg ppgmppphmm ngpigtgpgp ygpppgmgpp nmnnipgqqq pqqqqaqqqa
      181 nipgldlnge vwvetktpdg ksyyynirtr ettwtkpegp nvkvmvqdql eqlvhgaskq
      241 tvpsstptst attpsqisen rvsqsseqpp annadainql stappgtgpp ptlgetpnvn
      301 vqptdstqan gtapatvtnt ptmntpavnt nmmqpppnmi pmqhripnqf ggpiatqfga
      361 apfgmpppgf qpfggygppq anwgipqmph gvmtpqapae dpailaqldq elvasamvwt
      421 ehrapdgrly yynskagesv wekpqalkdl enaklalrqk aeeaavnstn tavtssavpn
      481 nnvttepikq dkpqesnhet kdsaketdtn kpkkeevapk eaakpqdksr pisstpvpgt
      541 pwcvvwtgdg rvffynpssr isvwerpddl igrqdvdkmv stppdavvat ktprqsdtse
      601 ssdddqptpa kkmkqedtks vtpkeeeeke skktidigke aaieaevraa reraivplet
      661 riksfrdmla ekevsafstw ekelhkivfd pryllltske rkqvfekyvk eraeeerrek
      721 rnkmkerkeq fqklleeagl hgkssfsdfa qkhgrderfk nvekmreres lfneyllevr
      781 kkekeektak reqvkkeffa mlrehkdidr hshwsdckkk lesdwryrvv esastredwf
      841 rdyirmlkee rkkekekdkd hrhrdkdhhk sdkkdrdrkd vdkykekssk drvekdsskd
      901 kkqrrseaps eengkekkes vvekesgeie dndekpskke ndkedaedqs dseedrekqk
      961 rererraeas lrererevqr tlathlrdrd kerqhhrhte avqhfsalla dlvrngdlaw
     1021 reakrqlrkd hrwelaesld reekerlfne hieqlsrkkr dkfrelldev gasteltasw
     1081 rdikkllkdd prylkfsssd rkcekefkey ikdklvaaka dfrellqetk litdktykkv
     1141 qensahlvei eeilrkdrrf lvleaaaaer trllmgylee larrgppppp tasepsrrpt
     1201 tn
//
LOCUS       XP_034183864            1176 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation regulator 1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183864
VERSION     XP_034183864.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327973.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1176
                     /product="transcription elongation regulator 1 isoform X4"
                     /calculated_mol_wt=134521
     Region          66..141
                     /region_name="Collagen"
                     /note="Collagen triple helix repeat (20 copies);
                     pfam01391"
                     /db_xref="CDD:460189"
     Region          166..191
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          275..355
                     /region_name="DUF1720"
                     /note="Domain of unknown function (DUF1720); pfam08226"
                     /db_xref="CDD:369766"
     Region          390..>844
                     /region_name="PRP40"
                     /note="Splicing factor [RNA processing and modification];
                     COG5104"
                     /db_xref="CDD:227435"
     Region          635..682
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          700..749
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          769..817
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          <850..>1159
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1176
                     /gene="LOC117605863"
                     /coded_by="XM_034327973.2:136..3666"
                     /db_xref="GeneID:117605863"
ORIGIN      
        1 metsteqtpv stetvpnneq ehneegmeet edysfdgedy rhfvprgrgg frgrgrggfd
       61 fpmrggpprf rgrgfgprgp mfrganngfp fegpprpncp pgpagprfrg pppfdpswgp
      121 mgppnlmgpp nlmgppgmip gqqqpqqqqa qqqanipgld lngevwvetk tpdgksyyyn
      181 irtrettwtk pegpnvkvmv qdqleqlvhg askqtvpsst ptstattpsq isenrvsqss
      241 eqppannada inqlstappg tgppptlget pnvnvqptds tqangtapat vtntptmntp
      301 avntnmmqpp pnmipmqhri pnqfggpiat qfgaapfgmp ppgfqpfggy gppqanwgip
      361 qmphgvmtpq apaedpaila qldqelvasa mvwtehrapd grlyyynska gesvwekpqa
      421 lkdlenakla lrqkaeeaav nstntavtss avpnnnvtte pikqdkpqes nhetkdsake
      481 tdtnkpkkee vapkeaakpq dksrpisstp vpgtpwcvvw tgdgrvffyn pssrisvwer
      541 pddligrqdv dkmvstppda vvatktprqs dtsessdddq ptpakkmkqe dtksvtpkee
      601 eekeskktid igkeaaieae vraareraiv pletriksfr dmlaekevsa fstwekelhk
      661 ivfdprylll tskerkqvfe kyvkeraeee rrekrnkmke rkeqfqklle eaglhgkssf
      721 sdfaqkhgrd erfknvekmr ereslfneyl levrkkekee ktakreqvkk effamlrehk
      781 didrhshwsd ckkklesdwr yrvvesastr edwfrdyirm lkeerkkeke kdkdhrhrdk
      841 dhhksdkkdr drkdvdkyke ksskdrvekd sskdkkqrrs eapseengke kkesvvekes
      901 geiedndekp skkendkeda edqsdseedr ekqkrererr aeaslrerer evqrtlathl
      961 rdrdkerqhh rhteavqhfs alladlvrng dlawreakrq lrkdhrwela esldreeker
     1021 lfnehieqls rkkrdkfrel ldevgastel taswrdikkl lkddprylkf sssdrkceke
     1081 fkeyikdklv aakadfrell qetklitdkt ykkvqensah lveieeilrk drrflvleaa
     1141 aaertrllmg yleelarrgp pppptaseps rrpttn
//
LOCUS       XP_034183946            1144 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation regulator 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183946
VERSION     XP_034183946.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328055.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1144
                     /product="transcription elongation regulator 1 isoform X5"
                     /calculated_mol_wt=130402
     Region          134..159
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          243..323
                     /region_name="DUF1720"
                     /note="Domain of unknown function (DUF1720); pfam08226"
                     /db_xref="CDD:369766"
     Region          358..>812
                     /region_name="PRP40"
                     /note="Splicing factor [RNA processing and modification];
                     COG5104"
                     /db_xref="CDD:227435"
     Region          603..650
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          668..717
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          737..785
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          <818..>1127
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1144
                     /gene="LOC117605863"
                     /coded_by="XM_034328055.2:181..3615"
                     /db_xref="GeneID:117605863"
ORIGIN      
        1 mrggpprfrg rgfgprgpmf rganngfpfe gpprpncppg pagprfrgpp pfdpswgpmg
       61 ppnlmgppnl mgppgmppph mmngpigtgp gpygpppgmg ppnmnnipgq qqpqqqqaqq
      121 qanipgldln gevwvetktp dgksyyynir trettwtkpe gpnvkvmvqd qleqlvhgas
      181 kqtvpsstpt stattpsqis enrvsqsseq ppannadain qlstappgtg ppptlgetpn
      241 vnvqptdstq angtapatvt ntptmntpav ntnmmqpppn mipmqhripn qfggpiatqf
      301 gaapfgmppp gfqpfggygp pqanwgipqm phgvmtpqap aedpailaql dqelvasamv
      361 wtehrapdgr lyyynskage svwekpqalk dlenaklalr qkaeeaavns tntavtssav
      421 pnnnvttepi kqdkpqesnh etkdsaketd tnkpkkeeva pkeaakpqdk srpisstpvp
      481 gtpwcvvwtg dgrvffynps srisvwerpd dligrqdvdk mvstppdavv atktprqsdt
      541 sessdddqpt pakkmkqedt ksvtpkeeee keskktidig keaaieaevr aareraivpl
      601 etriksfrdm laekevsafs twekelhkiv fdprylllts kerkqvfeky vkeraeeerr
      661 ekrnkmkerk eqfqklleea glhgkssfsd faqkhgrder fknvekmrer eslfneylle
      721 vrkkekeekt akreqvkkef famlrehkdi drhshwsdck kklesdwryr vvesastred
      781 wfrdyirmlk eerkkekekd kdhrhrdkdh hksdkkdrdr kdvdkykeks skdrvekdss
      841 kdkkqrrsea pseengkekk esvvekesge iedndekpsk kendkedaed qsdseedrek
      901 qkrererrae aslrererev qrtlathlrd rdkerqhhrh teavqhfsal ladlvrngdl
      961 awreakrqlr kdhrwelaes ldreekerlf nehieqlsrk krdkfrelld evgastelta
     1021 swrdikkllk ddprylkfss sdrkcekefk eyikdklvaa kadfrellqe tklitdktyk
     1081 kvqensahlv eieeilrkdr rflvleaaaa ertrllmgyl eelarrgppp pptasepsrr
     1141 pttn
//
LOCUS       XP_034184031            1144 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation regulator 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184031
VERSION     XP_034184031.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328140.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1144
                     /product="transcription elongation regulator 1 isoform X5"
                     /calculated_mol_wt=130402
     Region          134..159
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          243..323
                     /region_name="DUF1720"
                     /note="Domain of unknown function (DUF1720); pfam08226"
                     /db_xref="CDD:369766"
     Region          358..>812
                     /region_name="PRP40"
                     /note="Splicing factor [RNA processing and modification];
                     COG5104"
                     /db_xref="CDD:227435"
     Region          603..650
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          668..717
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          737..785
                     /region_name="FF"
                     /note="FF domain; pfam01846"
                     /db_xref="CDD:426471"
     Region          <818..>1127
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1144
                     /gene="LOC117605863"
                     /coded_by="XM_034328140.2:160..3594"
                     /db_xref="GeneID:117605863"
ORIGIN      
        1 mrggpprfrg rgfgprgpmf rganngfpfe gpprpncppg pagprfrgpp pfdpswgpmg
       61 ppnlmgppnl mgppgmppph mmngpigtgp gpygpppgmg ppnmnnipgq qqpqqqqaqq
      121 qanipgldln gevwvetktp dgksyyynir trettwtkpe gpnvkvmvqd qleqlvhgas
      181 kqtvpsstpt stattpsqis enrvsqsseq ppannadain qlstappgtg ppptlgetpn
      241 vnvqptdstq angtapatvt ntptmntpav ntnmmqpppn mipmqhripn qfggpiatqf
      301 gaapfgmppp gfqpfggygp pqanwgipqm phgvmtpqap aedpailaql dqelvasamv
      361 wtehrapdgr lyyynskage svwekpqalk dlenaklalr qkaeeaavns tntavtssav
      421 pnnnvttepi kqdkpqesnh etkdsaketd tnkpkkeeva pkeaakpqdk srpisstpvp
      481 gtpwcvvwtg dgrvffynps srisvwerpd dligrqdvdk mvstppdavv atktprqsdt
      541 sessdddqpt pakkmkqedt ksvtpkeeee keskktidig keaaieaevr aareraivpl
      601 etriksfrdm laekevsafs twekelhkiv fdprylllts kerkqvfeky vkeraeeerr
      661 ekrnkmkerk eqfqklleea glhgkssfsd faqkhgrder fknvekmrer eslfneylle
      721 vrkkekeekt akreqvkkef famlrehkdi drhshwsdck kklesdwryr vvesastred
      781 wfrdyirmlk eerkkekekd kdhrhrdkdh hksdkkdrdr kdvdkykeks skdrvekdss
      841 kdkkqrrsea pseengkekk esvvekesge iedndekpsk kendkedaed qsdseedrek
      901 qkrererrae aslrererev qrtlathlrd rdkerqhhrh teavqhfsal ladlvrngdl
      961 awreakrqlr kdhrwelaes ldreekerlf nehieqlsrk krdkfrelld evgastelta
     1021 swrdikkllk ddprylkfss sdrkcekefk eyikdklvaa kadfrellqe tklitdktyk
     1081 kvqensahlv eieeilrkdr rflvleaaaa ertrllmgyl eelarrgppp pptasepsrr
     1141 pttn
//
LOCUS       XP_076546390            1106 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase N isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546390
VERSION     XP_076546390.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690275.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1106
                     /product="serine/threonine-protein kinase N isoform X1"
                     /calculated_mol_wt=124555
     Region          67..130
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(73,76,79..80,83..84,86..87,90..91,93..94,109..110,
                     113,116..117,120)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(81..82,84..85,88..89,91..92,95..96,99,104..105,107)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          167..232
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(172,175,178..179,182..183,185..186,189..190,
                     192..193,211..212,215,218..219,222)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(180..181,183..184,187..188,190..191,194..195,
                     206..207,209)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          258..>315
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(268,271,274..275,278..279,281..282,285..286,
                     288..289,309..310,313)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(276..277,279..280,283..284,286..287,290..291,
                     304..305,307)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          440..521
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          779..1104
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(785..789,793,806,808,843,859..860,862,866,868,904,
                     906,908..909,911,921..922,925,939..944,971,977,980)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(785..789,793,806,808,843,860..862,866,904,906,
                     908..909,911,921..922)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(789,866,868,904,906,908,925,939..944,971,977,980)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            921..944
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1080
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1096..1101
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1106
                     /gene="Pkn"
                     /coded_by="XM_076690275.1:1279..4599"
                     /db_xref="GeneID:117609137"
ORIGIN      
        1 mscvsagkkg kkprmcrcls ttkackpkyf wtcilpcpgd yikhpvlyel sskygvagsd
       61 qvplpvrlde lrehirreir kelkikagae klrevatdrk alsdvasivk ksnsklnelq
      121 aelqqlesqi iltqgqpqsp qqnhsngqdt plspmgpssp sqeglftdlr llslekqlni
      181 elkvkqgaen miqsltngrd kkllqeaqqm lddsrakief lrmrimkvrq arqqqhargd
      241 apppngeats nkdryepsle laleerveel rhrlrieaav vegaknvirl lqsakvadkk
      301 alteaqasla essrkldllr lslelrrqel ppdsgtaaql krelasvqsa spvpvtytsl
      361 qpfrgplegr atvpasvsrc aavtgqlevr lmgcqdlaee vpgrtrrehp aspdlrsfvk
      421 gvtgrsssks ysvkdetsnd imavikldnq tvaqtswrpc sqqawdqrfs ieldksrele
      481 igiywkdwrs lcaikflrle efiddvrhgm alqlepqgll faeikflnpm isrkpklqrq
      541 rkifkqqvkn fpranqmnin vatwgrllkr sapslhntrn sesppsgssv yysfvfihis
      601 tddqlfiffi gaqplqlvfd tsledkpetp gelpdpekgg lggarplgls tssssptlpr
      661 ppehpppppp tvtkkpslpa pppppkldqe lqsalrefdf lhneekggpq gailrplvte
      721 prpvliapps prtpspqpvv efpedevvye mpskpsqyrd sayesrrhsq ltgmtidnfr
      781 llsvlgrghf gkvilsqyrn tgeyfaikal kkgdiiarde vesllsekri fevanatrhp
      841 flvnlfacfq teahvcfvme yaaggdlmmh ihadvfgepr avfysacvvl glqylhesri
      901 iyrdlkldnl lldtegyvki adfglckegm gygdrtgtfc gtpeflapev ltetsytrav
      961 dwwglgvlif emlvgespfp gddeeevfds ivndevrypr flsleaiaim rrllrknpdr
     1021 rlgsserdae dvkkqaffrh iawddlllrr vkppfvpvih svedvsnfde eftsekpqlt
     1081 ppkdprplnd leqmlfkdft ymadwc
//
LOCUS       XP_076546391            1106 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase N isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546391
VERSION     XP_076546391.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690276.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1106
                     /product="serine/threonine-protein kinase N isoform X1"
                     /calculated_mol_wt=124555
     Region          67..130
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(73,76,79..80,83..84,86..87,90..91,93..94,109..110,
                     113,116..117,120)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(81..82,84..85,88..89,91..92,95..96,99,104..105,107)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          167..232
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(172,175,178..179,182..183,185..186,189..190,
                     192..193,211..212,215,218..219,222)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(180..181,183..184,187..188,190..191,194..195,
                     206..207,209)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          258..>315
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(268,271,274..275,278..279,281..282,285..286,
                     288..289,309..310,313)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(276..277,279..280,283..284,286..287,290..291,
                     304..305,307)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          440..521
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          779..1104
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(785..789,793,806,808,843,859..860,862,866,868,904,
                     906,908..909,911,921..922,925,939..944,971,977,980)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(785..789,793,806,808,843,860..862,866,904,906,
                     908..909,911,921..922)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(789,866,868,904,906,908,925,939..944,971,977,980)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            921..944
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1080
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1096..1101
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1106
                     /gene="Pkn"
                     /coded_by="XM_076690276.1:605..3925"
                     /db_xref="GeneID:117609137"
ORIGIN      
        1 mscvsagkkg kkprmcrcls ttkackpkyf wtcilpcpgd yikhpvlyel sskygvagsd
       61 qvplpvrlde lrehirreir kelkikagae klrevatdrk alsdvasivk ksnsklnelq
      121 aelqqlesqi iltqgqpqsp qqnhsngqdt plspmgpssp sqeglftdlr llslekqlni
      181 elkvkqgaen miqsltngrd kkllqeaqqm lddsrakief lrmrimkvrq arqqqhargd
      241 apppngeats nkdryepsle laleerveel rhrlrieaav vegaknvirl lqsakvadkk
      301 alteaqasla essrkldllr lslelrrqel ppdsgtaaql krelasvqsa spvpvtytsl
      361 qpfrgplegr atvpasvsrc aavtgqlevr lmgcqdlaee vpgrtrrehp aspdlrsfvk
      421 gvtgrsssks ysvkdetsnd imavikldnq tvaqtswrpc sqqawdqrfs ieldksrele
      481 igiywkdwrs lcaikflrle efiddvrhgm alqlepqgll faeikflnpm isrkpklqrq
      541 rkifkqqvkn fpranqmnin vatwgrllkr sapslhntrn sesppsgssv yysfvfihis
      601 tddqlfiffi gaqplqlvfd tsledkpetp gelpdpekgg lggarplgls tssssptlpr
      661 ppehpppppp tvtkkpslpa pppppkldqe lqsalrefdf lhneekggpq gailrplvte
      721 prpvliapps prtpspqpvv efpedevvye mpskpsqyrd sayesrrhsq ltgmtidnfr
      781 llsvlgrghf gkvilsqyrn tgeyfaikal kkgdiiarde vesllsekri fevanatrhp
      841 flvnlfacfq teahvcfvme yaaggdlmmh ihadvfgepr avfysacvvl glqylhesri
      901 iyrdlkldnl lldtegyvki adfglckegm gygdrtgtfc gtpeflapev ltetsytrav
      961 dwwglgvlif emlvgespfp gddeeevfds ivndevrypr flsleaiaim rrllrknpdr
     1021 rlgsserdae dvkkqaffrh iawddlllrr vkppfvpvih svedvsnfde eftsekpqlt
     1081 ppkdprplnd leqmlfkdft ymadwc
//
LOCUS       XP_076546392            1082 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase N isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546392
VERSION     XP_076546392.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690277.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1082
                     /product="serine/threonine-protein kinase N isoform X2"
                     /calculated_mol_wt=121727
     Region          67..130
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(73,76,79..80,83..84,86..87,90..91,93..94,109..110,
                     113,116..117,120)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(81..82,84..85,88..89,91..92,95..96,99,104..105,107)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          167..232
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(172,175,178..179,182..183,185..186,189..190,
                     192..193,211..212,215,218..219,222)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(180..181,183..184,187..188,190..191,194..195,
                     206..207,209)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          258..>315
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(268,271,274..275,278..279,281..282,285..286,
                     288..289,309..310,313)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(276..277,279..280,283..284,286..287,290..291,
                     304..305,307)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          440..521
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          755..1080
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(761..765,769,782,784,819,835..836,838,842,844,880,
                     882,884..885,887,897..898,901,915..920,947,953,956)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(761..765,769,782,784,819,836..838,842,880,882,
                     884..885,887,897..898)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(765,842,844,880,882,884,901,915..920,947,953,956)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            897..920
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1056
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1072..1077
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1082
                     /gene="Pkn"
                     /coded_by="XM_076690277.1:934..4182"
                     /db_xref="GeneID:117609137"
ORIGIN      
        1 mscvsagkkg kkprmcrcls ttkackpkyf wtcilpcpgd yikhpvlyel sskygvagsd
       61 qvplpvrlde lrehirreir kelkikagae klrevatdrk alsdvasivk ksnsklnelq
      121 aelqqlesqi iltqgqpqsp qqnhsngqdt plspmgpssp sqeglftdlr llslekqlni
      181 elkvkqgaen miqsltngrd kkllqeaqqm lddsrakief lrmrimkvrq arqqqhargd
      241 apppngeats nkdryepsle laleerveel rhrlrieaav vegaknvirl lqsakvadkk
      301 alteaqasla essrkldllr lslelrrqel ppdsgtaaql krelasvqsa spvpvtytsl
      361 qpfrgplegr atvpasvsrc aavtgqlevr lmgcqdlaee vpgrtrrehp aspdlrsfvk
      421 gvtgrsssks ysvkdetsnd imavikldnq tvaqtswrpc sqqawdqrfs ieldksrele
      481 igiywkdwrs lcaikflrle efiddvrhgm alqlepqgll faeikflnpm isrkpklqrq
      541 rkifkqqvkn fpranqmnin vatwgrllkr sapslhntrn sesppsgaqp lqlvfdtsle
      601 dkpetpgelp dpekgglgga rplglstsss sptlprppeh pppppptvtk kpslpapppp
      661 pkldqelqsa lrefdflhne ekggpqgail rplvteprpv liappsprtp spqpvvefpe
      721 devvyempsk psqyrdsaye srrhsqltgm tidnfrllsv lgrghfgkvi lsqyrntgey
      781 faikalkkgd iiardevesl lsekrifeva natrhpflvn lfacfqteah vcfvmeyaag
      841 gdlmmhihad vfgepravfy sacvvlglqy lhesriiyrd lkldnllldt egyvkiadfg
      901 lckegmgygd rtgtfcgtpe flapevltet sytravdwwg lgvlifemlv gespfpgdde
      961 eevfdsivnd evryprflsl eaiaimrrll rknpdrrlgs serdaedvkk qaffrhiawd
     1021 dlllrrvkpp fvpvihsved vsnfdeefts ekpqltppkd prplndleqm lfkdftymad
     1081 wc
//
LOCUS       XP_076546393            1080 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase N isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546393
VERSION     XP_076546393.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690278.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1080
                     /product="serine/threonine-protein kinase N isoform X3"
                     /calculated_mol_wt=121486
     Region          67..130
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(73,76,79..80,83..84,86..87,90..91,93..94,109..110,
                     113,116..117,120)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(81..82,84..85,88..89,91..92,95..96,99,104..105,107)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          167..232
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(172,175,178..179,182..183,185..186,189..190,
                     192..193,211..212,215,218..219,222)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(180..181,183..184,187..188,190..191,194..195,
                     206..207,209)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          258..>315
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(268,271,274..275,278..279,281..282,285..286,
                     288..289,309..310,313)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(276..277,279..280,283..284,286..287,290..291,
                     304..305,307)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          440..521
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          753..1078
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(759..763,767,780,782,817,833..834,836,840,842,878,
                     880,882..883,885,895..896,899,913..918,945,951,954)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(759..763,767,780,782,817,834..836,840,878,880,
                     882..883,885,895..896)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(763,840,842,878,880,882,899,913..918,945,951,954)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            895..918
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1054
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1070..1075
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1080
                     /gene="Pkn"
                     /coded_by="XM_076690278.1:935..4177"
                     /db_xref="GeneID:117609137"
ORIGIN      
        1 mscvsagkkg kkprmcrcls ttkackpkyf wtcilpcpgd yikhpvlyel sskygvagsd
       61 qvplpvrlde lrehirreir kelkikagae klrevatdrk alsdvasivk ksnsklnelq
      121 aelqqlesqi iltqgqpqsp qqnhsngqdt plspmgpssp sqeglftdlr llslekqlni
      181 elkvkqgaen miqsltngrd kkllqeaqqm lddsrakief lrmrimkvrq arqqqhargd
      241 apppngeats nkdryepsle laleerveel rhrlrieaav vegaknvirl lqsakvadkk
      301 alteaqasla essrkldllr lslelrrqel ppdsgtaaql krelasvqsa spvpvtytsl
      361 qpfrgplegr atvpasvsrc aavtgqlevr lmgcqdlaee vpgrtrrehp aspdlrsfvk
      421 gvtgrsssks ysvkdetsnd imavikldnq tvaqtswrpc sqqawdqrfs ieldksrele
      481 igiywkdwrs lcaikflrle efiddvrhgm alqlepqgll faeikflnpm isrkpklqrq
      541 rkifkqqvkn fpranqmnin vatwgrllkr sapslhntrn sesppsgaqp lqlvfdtsle
      601 dkpetpgelp dpekgglgga rplglstsss sptlprppeh pppppptvtk kpslpapppp
      661 pkldqesalr efdflhneek ggpqgailrp lvteprpvli appsprtpsp qpvvefpede
      721 vvyempskps qyrdsayesr rhsqltgmti dnfrllsvlg rghfgkvils qyrntgeyfa
      781 ikalkkgdii ardeveslls ekrifevana trhpflvnlf acfqteahvc fvmeyaaggd
      841 lmmhihadvf gepravfysa cvvlglqylh esriiyrdlk ldnllldteg yvkiadfglc
      901 kegmgygdrt gtfcgtpefl apevltetsy travdwwglg vlifemlvge spfpgddeee
      961 vfdsivndev ryprflslea iaimrrllrk npdrrlgsse rdaedvkkqa ffrhiawddl
     1021 llrrvkppfv pvihsvedvs nfdeeftsek pqltppkdpr plndleqmlf kdftymadwc
//
LOCUS       XP_076546394            1076 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase N isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546394
VERSION     XP_076546394.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690279.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1076
                     /product="serine/threonine-protein kinase N isoform X4"
                     /calculated_mol_wt=121280
     Region          37..100
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(43,46,49..50,53..54,56..57,60..61,63..64,79..80,83,
                     86..87,90)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(51..52,54..55,58..59,61..62,65..66,69,74..75,77)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          137..202
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(142,145,148..149,152..153,155..156,159..160,
                     162..163,181..182,185,188..189,192)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(150..151,153..154,157..158,160..161,164..165,
                     176..177,179)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          228..>285
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(238,241,244..245,248..249,251..252,255..256,
                     258..259,279..280,283)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(246..247,249..250,253..254,256..257,260..261,
                     274..275,277)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          410..491
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          749..1074
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(755..759,763,776,778,813,829..830,832,836,838,874,
                     876,878..879,881,891..892,895,909..914,941,947,950)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(755..759,763,776,778,813,830..832,836,874,876,
                     878..879,881,891..892)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(759,836,838,874,876,878,895,909..914,941,947,950)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            891..914
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1050
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1066..1071
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1076
                     /gene="Pkn"
                     /coded_by="XM_076690279.1:285..3515"
                     /db_xref="GeneID:117609137"
ORIGIN      
        1 maessyyqgd yikhpvlyel sskygvagsd qvplpvrlde lrehirreir kelkikagae
       61 klrevatdrk alsdvasivk ksnsklnelq aelqqlesqi iltqgqpqsp qqnhsngqdt
      121 plspmgpssp sqeglftdlr llslekqlni elkvkqgaen miqsltngrd kkllqeaqqm
      181 lddsrakief lrmrimkvrq arqqqhargd apppngeats nkdryepsle laleerveel
      241 rhrlrieaav vegaknvirl lqsakvadkk alteaqasla essrkldllr lslelrrqel
      301 ppdsgtaaql krelasvqsa spvpvtytsl qpfrgplegr atvpasvsrc aavtgqlevr
      361 lmgcqdlaee vpgrtrrehp aspdlrsfvk gvtgrsssks ysvkdetsnd imavikldnq
      421 tvaqtswrpc sqqawdqrfs ieldksrele igiywkdwrs lcaikflrle efiddvrhgm
      481 alqlepqgll faeikflnpm isrkpklqrq rkifkqqvkn fpranqmnin vatwgrllkr
      541 sapslhntrn sesppsgssv yysfvfihis tddqlfiffi gaqplqlvfd tsledkpetp
      601 gelpdpekgg lggarplgls tssssptlpr ppehpppppp tvtkkpslpa pppppkldqe
      661 lqsalrefdf lhneekggpq gailrplvte prpvliapps prtpspqpvv efpedevvye
      721 mpskpsqyrd sayesrrhsq ltgmtidnfr llsvlgrghf gkvilsqyrn tgeyfaikal
      781 kkgdiiarde vesllsekri fevanatrhp flvnlfacfq teahvcfvme yaaggdlmmh
      841 ihadvfgepr avfysacvvl glqylhesri iyrdlkldnl lldtegyvki adfglckegm
      901 gygdrtgtfc gtpeflapev ltetsytrav dwwglgvlif emlvgespfp gddeeevfds
      961 ivndevrypr flsleaiaim rrllrknpdr rlgsserdae dvkkqaffrh iawddlllrr
     1021 vkppfvpvih svedvsnfde eftsekpqlt ppkdprplnd leqmlfkdft ymadwc
//
LOCUS       XP_034190997            1052 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase N isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190997
VERSION     XP_034190997.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335106.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190997.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1052
                     /product="serine/threonine-protein kinase N isoform X5"
                     /calculated_mol_wt=118452
     Region          37..100
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(43,46,49..50,53..54,56..57,60..61,63..64,79..80,83,
                     86..87,90)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(51..52,54..55,58..59,61..62,65..66,69,74..75,77)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          137..202
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(142,145,148..149,152..153,155..156,159..160,
                     162..163,181..182,185,188..189,192)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(150..151,153..154,157..158,160..161,164..165,
                     176..177,179)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          228..>285
                     /region_name="HR1"
                     /note="Protein kinase C-related kinase homology region 1
                     (HR1) domain that binds Rho family small GTPases; cl00087"
                     /db_xref="CDD:469609"
     Site            order(238,241,244..245,248..249,251..252,255..256,
                     258..259,279..280,283)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(246..247,249..250,253..254,256..257,260..261,
                     274..275,277)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          410..491
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          725..1050
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(731..735,739,752,754,789,805..806,808,812,814,850,
                     852,854..855,857,867..868,871,885..890,917,923,926)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(731..735,739,752,754,789,806..808,812,850,852,
                     854..855,857,867..868)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(735,812,814,850,852,854,871,885..890,917,923,926)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            867..890
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1026
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1042..1047
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1052
                     /gene="Pkn"
                     /coded_by="XM_034335106.2:285..3443"
                     /db_xref="GeneID:117609137"
ORIGIN      
        1 maessyyqgd yikhpvlyel sskygvagsd qvplpvrlde lrehirreir kelkikagae
       61 klrevatdrk alsdvasivk ksnsklnelq aelqqlesqi iltqgqpqsp qqnhsngqdt
      121 plspmgpssp sqeglftdlr llslekqlni elkvkqgaen miqsltngrd kkllqeaqqm
      181 lddsrakief lrmrimkvrq arqqqhargd apppngeats nkdryepsle laleerveel
      241 rhrlrieaav vegaknvirl lqsakvadkk alteaqasla essrkldllr lslelrrqel
      301 ppdsgtaaql krelasvqsa spvpvtytsl qpfrgplegr atvpasvsrc aavtgqlevr
      361 lmgcqdlaee vpgrtrrehp aspdlrsfvk gvtgrsssks ysvkdetsnd imavikldnq
      421 tvaqtswrpc sqqawdqrfs ieldksrele igiywkdwrs lcaikflrle efiddvrhgm
      481 alqlepqgll faeikflnpm isrkpklqrq rkifkqqvkn fpranqmnin vatwgrllkr
      541 sapslhntrn sesppsgaqp lqlvfdtsle dkpetpgelp dpekgglgga rplglstsss
      601 sptlprppeh pppppptvtk kpslpapppp pkldqelqsa lrefdflhne ekggpqgail
      661 rplvteprpv liappsprtp spqpvvefpe devvyempsk psqyrdsaye srrhsqltgm
      721 tidnfrllsv lgrghfgkvi lsqyrntgey faikalkkgd iiardevesl lsekrifeva
      781 natrhpflvn lfacfqteah vcfvmeyaag gdlmmhihad vfgepravfy sacvvlglqy
      841 lhesriiyrd lkldnllldt egyvkiadfg lckegmgygd rtgtfcgtpe flapevltet
      901 sytravdwwg lgvlifemlv gespfpgdde eevfdsivnd evryprflsl eaiaimrrll
      961 rknpdrrlgs serdaedvkk qaffrhiawd dlllrrvkpp fvpvihsved vsnfdeefts
     1021 ekpqltppkd prplndleqm lfkdftymad wc
//
LOCUS       XP_076546395            1026 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase N isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546395
VERSION     XP_076546395.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690280.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1026
                     /product="serine/threonine-protein kinase N isoform X6"
                     /calculated_mol_wt=115545
     Region          67..130
                     /region_name="HR1_PKN_1"
                     /note="First Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11622"
                     /db_xref="CDD:212012"
     Site            order(73,76,79..80,83..84,86..87,90..91,93..94,109..110,
                     113,116..117,120)
                     /site_type="other"
                     /note="Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Site            order(81..82,84..85,88..89,91..92,95..96,99,104..105,107)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212012"
     Region          167..232
                     /region_name="HR1_PKN_2"
                     /note="Second Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11623"
                     /db_xref="CDD:212013"
     Site            order(172,175,178..179,182..183,185..186,189..190,
                     192..193,211..212,215,218..219,222)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Site            order(180..181,183..184,187..188,190..191,194..195,
                     206..207,209)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212013"
     Region          258..>315
                     /region_name="HR1_PKN_3"
                     /note="Third Protein kinase C-related kinase homology
                     region 1 (HR1) Rho-binding domain of Protein Kinase N;
                     cd11625"
                     /db_xref="CDD:212015"
     Site            order(268,271,274..275,278..279,281..282,285..286,
                     288..289,309..310,313)
                     /site_type="other"
                     /note="putative Rho binding site 1 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Site            order(276..277,279..280,283..284,286..287,290..291,
                     304..305,307)
                     /site_type="other"
                     /note="putative Rho binding site 2 [polypeptide binding]"
                     /db_xref="CDD:212015"
     Region          440..521
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          699..1024
                     /region_name="STKc_PKN"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Protein Kinase N; cd05589"
                     /db_xref="CDD:270741"
     Site            order(705..709,713,726,728,763,779..780,782,786,788,824,
                     826,828..829,831,841..842,845,859..864,891,897,900)
                     /site_type="active"
                     /db_xref="CDD:270741"
     Site            order(705..709,713,726,728,763,780..782,786,824,826,
                     828..829,831,841..842)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270741"
     Site            order(709,786,788,824,826,828,845,859..864,891,897,900)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270741"
     Site            841..864
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270741"
     Site            1000
                     /site_type="other"
                     /note="turn motif phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:270741"
     Site            1016..1021
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270741"
     CDS             1..1026
                     /gene="Pkn"
                     /coded_by="XM_076690280.1:934..4014"
                     /db_xref="GeneID:117609137"
ORIGIN      
        1 mscvsagkkg kkprmcrcls ttkackpkyf wtcilpcpgd yikhpvlyel sskygvagsd
       61 qvplpvrlde lrehirreir kelkikagae klrevatdrk alsdvasivk ksnsklnelq
      121 aelqqlesqi iltqgqpqsp qqnhsngqdt plspmgpssp sqeglftdlr llslekqlni
      181 elkvkqgaen miqsltngrd kkllqeaqqm lddsrakief lrmrimkvrq arqqqhargd
      241 apppngeats nkdryepsle laleerveel rhrlrieaav vegaknvirl lqsakvadkk
      301 alteaqasla essrkldllr lslelrrqel ppdsgtaaql krelasvqsa spvpvtytsl
      361 qpfrgplegr atvpasvsrc aavtgqlevr lmgcqdlaee vpgrtrrehp aspdlrsfvk
      421 gvtgrsssks ysvkdetsnd imavikldnq tvaqtswrpc sqqawdqrfs ieldksrele
      481 igiywkdwrs lcaikflrle efiddvrhgm alqlepqgll faeikflnpm isrkpklqrq
      541 rkifkqqvkn fpranqmnin vatwgrllkr sapslhntrn sesppsgaqp lqlvfdtsle
      601 dkpetpgelp dpekgglgga rplglstsss sptlprppeh pppppptvtk kpslpapppp
      661 pkldqevvye mpskpsqyrd sayesrrhsq ltgmtidnfr llsvlgrghf gkvilsqyrn
      721 tgeyfaikal kkgdiiarde vesllsekri fevanatrhp flvnlfacfq teahvcfvme
      781 yaaggdlmmh ihadvfgepr avfysacvvl glqylhesri iyrdlkldnl lldtegyvki
      841 adfglckegm gygdrtgtfc gtpeflapev ltetsytrav dwwglgvlif emlvgespfp
      901 gddeeevfds ivndevrypr flsleaiaim rrllrknpdr rlgsserdae dvkkqaffrh
      961 iawddlllrr vkppfvpvih svedvsnfde eftsekpqlt ppkdprplnd leqmlfkdft
     1021 ymadwc
//
LOCUS       XP_034191020             726 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609153 [Osmia lignaria lignaria].
ACCESSION   XP_034191020
VERSION     XP_034191020.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335129.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191020.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..726
                     /product="uncharacterized protein LOC117609153"
                     /calculated_mol_wt=80357
     CDS             1..726
                     /gene="LOC117609153"
                     /coded_by="XM_034335129.2:2110..4290"
                     /db_xref="GeneID:117609153"
ORIGIN      
        1 mygrqgnsin sqpppwlvra evtihhgapv tsesgqvpvs msstvtdsng vrmiyrwntd
       61 qsspstvpat asipspssss sfgfqrgnil ftstppprpt prsftiprss glsenesssr
      121 grganvsdsg ynseqfssqs ysslpsrrps qqynrrcrst csivlsavnt edsvsrkess
      181 sesirhtydn swrhhsssrq hvftrhqfst vpevceestd gnntssayst hfcgrlkdkl
      241 ssesttvskd assqttdves qeststivsk sttrrkttgg lrssneqktk qqesrspttg
      301 settgvfpsa dseksdssak desakrksrt vhidvyctgs dddensdtss endretpmtv
      361 fenpdvkvvh tqapdnvlpr gfqddnafmk raterrcdsf knapmrmpsi asskgydsdd
      421 vlsslypsqf ssysalrdld sapwsaassn agipfdydst vatspkdtfs didslinsrv
      481 altpcdsfey asstdrerir rmeeiwskse wpierehllr nrrtrehpgi retewssads
      541 gedsdesgtv gwsfvssees qrmtkktsti rrtsksgect eknvpveket vqrglqqdqs
      601 dsttrsdsip hpyafrdqvg pfvsnspspl pskvpsrvts pfmtpqgert nhilkasifg
      661 svvnafrkpg hhigpsknps cacehcrrhf eelnsrerss svsdferqtg frlqsekhiv
      721 rpipkk
//
LOCUS       XP_076546397             627 aa            linear   INV 03-SEP-2025
DEFINITION  conserved oligomeric Golgi complex subunit 6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546397
VERSION     XP_076546397.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690282.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="conserved oligomeric Golgi complex subunit 6"
                     /calculated_mol_wt=70987
     Region          31..620
                     /region_name="COG6"
                     /note="Conserved oligomeric complex COG6; pfam06419"
                     /db_xref="CDD:461904"
     CDS             1..627
                     /gene="Cog6"
                     /coded_by="XM_076690282.1:464..2347"
                     /db_xref="GeneID:117609158"
ORIGIN      
        1 mseknpntal vrrvnklles rvehdkdtle alkelstfft entlnsrrnl rskierrsla
       61 ineeflsafr evksslddly qdvlamnttv qcmtnrlqvt kaqtsqlidq ttklqsksqt
      121 lsmhhevana fiknfqltss elnilhgstr espiteeffs vvnrvqeihs ncrvlmqsgy
      181 qtlaldimqr mtllqeaale rlyrwtqnqc knienehlav llikamsklq drpvlfkyil
      241 deycaarrta lvgsfidalt lgegysttpn piemhandpk ryigdmlawl hqaipveken
      301 iltlvkgcdk tdlsdqikqc lsniteglch plksriehvi smdapatvly sittllrfyr
      361 aiiqqvipds tldltladll ilsersflsr lqketrallg eraeppgndl vpapsvsrlm
      421 allneilsva siaedrekdm qqivsciidp llqeinetas rlptvdmavy llncmhqiqs
      481 tlalyeymdq rlerlqaqsd aqidtltseq asslvahlnl gsiytilqgh eqgplssvpg
      541 mdslsvkeft nkleaflvmp dvlllpqinl lqsnnfrsit qkrsfeviga iykqlyeach
      601 dpknlyqnpt slfsrtpedl lktlisi
//
LOCUS       XP_034191030             627 aa            linear   INV 03-SEP-2025
DEFINITION  conserved oligomeric Golgi complex subunit 6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191030
VERSION     XP_034191030.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335139.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="conserved oligomeric Golgi complex subunit 6"
                     /calculated_mol_wt=70987
     Region          31..620
                     /region_name="COG6"
                     /note="Conserved oligomeric complex COG6; pfam06419"
                     /db_xref="CDD:461904"
     CDS             1..627
                     /gene="Cog6"
                     /coded_by="XM_034335139.2:464..2347"
                     /db_xref="GeneID:117609158"
ORIGIN      
        1 mseknpntal vrrvnklles rvehdkdtle alkelstfft entlnsrrnl rskierrsla
       61 ineeflsafr evksslddly qdvlamnttv qcmtnrlqvt kaqtsqlidq ttklqsksqt
      121 lsmhhevana fiknfqltss elnilhgstr espiteeffs vvnrvqeihs ncrvlmqsgy
      181 qtlaldimqr mtllqeaale rlyrwtqnqc knienehlav llikamsklq drpvlfkyil
      241 deycaarrta lvgsfidalt lgegysttpn piemhandpk ryigdmlawl hqaipveken
      301 iltlvkgcdk tdlsdqikqc lsniteglch plksriehvi smdapatvly sittllrfyr
      361 aiiqqvipds tldltladll ilsersflsr lqketrallg eraeppgndl vpapsvsrlm
      421 allneilsva siaedrekdm qqivsciidp llqeinetas rlptvdmavy llncmhqiqs
      481 tlalyeymdq rlerlqaqsd aqidtltseq asslvahlnl gsiytilqgh eqgplssvpg
      541 mdslsvkeft nkleaflvmp dvlllpqinl lqsnnfrsit qkrsfeviga iykqlyeach
      601 dpknlyqnpt slfsrtpedl lktlisi
//
LOCUS       XP_034191041             182 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609166 [Osmia lignaria lignaria].
ACCESSION   XP_034191041
VERSION     XP_034191041.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335150.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..182
                     /product="uncharacterized protein LOC117609166"
                     /calculated_mol_wt=20855
     Region          30..117
                     /region_name="Sen15"
                     /note="Sen15 protein; pfam09631"
                     /db_xref="CDD:462844"
     CDS             1..182
                     /gene="LOC117609166"
                     /coded_by="XM_034335150.2:197..745"
                     /db_xref="GeneID:117609166"
ORIGIN      
        1 mydichpsyy sieklgctdp vkisttfyay ielceakryw evnykfnevl dllylevkrn
       61 knsqteiymp wpashnisld kiekiqegln tdqvtfvfks adstsiiyka skglvkpmap
      121 ettklmkeke ekksnlekei rkntsylyel akslntentd ntenkkdtsp stsnsnntgi
      181 ng
//
LOCUS       XP_034188029            1052 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034188029
VERSION     XP_034188029.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1052
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X1"
                     /calculated_mol_wt=118733
     Region          300..478
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          577..915
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..1052
                     /gene="LOC117607900"
                     /coded_by="XM_034332138.2:845..4003"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mmcldiklvs rlrgvsstss atnvsattps satstsstta flekckrgkg rrestgdass
       61 vtesdseeet lpigpemirr nrsrsvcvsa lssmhqghlr rrashhvstn dtisrrgvls
      121 rryygsveml pqieqdgydn grrfrvengd spgekeemfg spstpilenp eyqtrwyfky
      181 flgklhqnyi aadpernclf lsvvtnevsd qsvphyrvil wtksgaqkis lpysanktit
      241 irqilsnffg ldkldkapre vfapeiqkdl llleeqegsv nfkfgiiyak kgqttddeml
      301 snekgspdfd kfleilgeri rlknwdkyrg gldvkgdmtg kesyytvyag hevmyhvstm
      361 lpyskdnpqq lerkrhignd ivniiytddp naidtfnpnc irsqfthvfa vvstedngkg
      421 wrvaifcdes vplfgpslpc ppvfedpytl refllvklin gekatfntpt faqkrertld
      481 allrdmyqeh sqdsksnsml nrralsdvve apgrrreetr avemvqvgqa lkleaivrgl
      541 aptslatagp lkprpweprc iypdfpheiv cgdvwldnrl ilasengtfl ledsishrli
      601 fdesvqikql dvveqhgill frtgdkgkes nvyvfrlkef enntgnrsae vpndreedqd
      661 gedngededa eddgdesedn dcdnftrata kfkpvirrtr arvrplavrg rthikerklp
      721 rtrgcnlysi tmpggshlrm cvvvgrrltv lqwkhsaawt awcsaadtdt idgflfvkef
      781 nvnetpslvt imentnnewt lccgvrhhfe lisssgstrv lhiegtpkph lvaalnlced
      841 eepelllcyn ntchfqkiwe esttptefdf nwnsvpaaia cafpyiiaft ndtmeirlii
      901 ngnlvhtiam pnlnlftskq diffsttape flpgkcerir ldtkpadkee pvsspspftq
      961 sssssrtnlq nnhgewkpir iyqiplqtls rstlsncsqr rcpspaepev vsapptsdks
     1021 flsvephral srscsssptp tptnlippri rk
//
LOCUS       XP_034188039            1052 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034188039
VERSION     XP_034188039.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332148.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1052
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X1"
                     /calculated_mol_wt=118733
     Region          300..478
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          577..915
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..1052
                     /gene="LOC117607900"
                     /coded_by="XM_034332148.2:841..3999"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mmcldiklvs rlrgvsstss atnvsattps satstsstta flekckrgkg rrestgdass
       61 vtesdseeet lpigpemirr nrsrsvcvsa lssmhqghlr rrashhvstn dtisrrgvls
      121 rryygsveml pqieqdgydn grrfrvengd spgekeemfg spstpilenp eyqtrwyfky
      181 flgklhqnyi aadpernclf lsvvtnevsd qsvphyrvil wtksgaqkis lpysanktit
      241 irqilsnffg ldkldkapre vfapeiqkdl llleeqegsv nfkfgiiyak kgqttddeml
      301 snekgspdfd kfleilgeri rlknwdkyrg gldvkgdmtg kesyytvyag hevmyhvstm
      361 lpyskdnpqq lerkrhignd ivniiytddp naidtfnpnc irsqfthvfa vvstedngkg
      421 wrvaifcdes vplfgpslpc ppvfedpytl refllvklin gekatfntpt faqkrertld
      481 allrdmyqeh sqdsksnsml nrralsdvve apgrrreetr avemvqvgqa lkleaivrgl
      541 aptslatagp lkprpweprc iypdfpheiv cgdvwldnrl ilasengtfl ledsishrli
      601 fdesvqikql dvveqhgill frtgdkgkes nvyvfrlkef enntgnrsae vpndreedqd
      661 gedngededa eddgdesedn dcdnftrata kfkpvirrtr arvrplavrg rthikerklp
      721 rtrgcnlysi tmpggshlrm cvvvgrrltv lqwkhsaawt awcsaadtdt idgflfvkef
      781 nvnetpslvt imentnnewt lccgvrhhfe lisssgstrv lhiegtpkph lvaalnlced
      841 eepelllcyn ntchfqkiwe esttptefdf nwnsvpaaia cafpyiiaft ndtmeirlii
      901 ngnlvhtiam pnlnlftskq diffsttape flpgkcerir ldtkpadkee pvsspspftq
      961 sssssrtnlq nnhgewkpir iyqiplqtls rstlsncsqr rcpspaepev vsapptsdks
     1021 flsvephral srscsssptp tptnlippri rk
//
LOCUS       XP_034188055            1052 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034188055
VERSION     XP_034188055.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332164.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1052
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X1"
                     /calculated_mol_wt=118733
     Region          300..478
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          577..915
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..1052
                     /gene="LOC117607900"
                     /coded_by="XM_034332164.2:688..3846"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mmcldiklvs rlrgvsstss atnvsattps satstsstta flekckrgkg rrestgdass
       61 vtesdseeet lpigpemirr nrsrsvcvsa lssmhqghlr rrashhvstn dtisrrgvls
      121 rryygsveml pqieqdgydn grrfrvengd spgekeemfg spstpilenp eyqtrwyfky
      181 flgklhqnyi aadpernclf lsvvtnevsd qsvphyrvil wtksgaqkis lpysanktit
      241 irqilsnffg ldkldkapre vfapeiqkdl llleeqegsv nfkfgiiyak kgqttddeml
      301 snekgspdfd kfleilgeri rlknwdkyrg gldvkgdmtg kesyytvyag hevmyhvstm
      361 lpyskdnpqq lerkrhignd ivniiytddp naidtfnpnc irsqfthvfa vvstedngkg
      421 wrvaifcdes vplfgpslpc ppvfedpytl refllvklin gekatfntpt faqkrertld
      481 allrdmyqeh sqdsksnsml nrralsdvve apgrrreetr avemvqvgqa lkleaivrgl
      541 aptslatagp lkprpweprc iypdfpheiv cgdvwldnrl ilasengtfl ledsishrli
      601 fdesvqikql dvveqhgill frtgdkgkes nvyvfrlkef enntgnrsae vpndreedqd
      661 gedngededa eddgdesedn dcdnftrata kfkpvirrtr arvrplavrg rthikerklp
      721 rtrgcnlysi tmpggshlrm cvvvgrrltv lqwkhsaawt awcsaadtdt idgflfvkef
      781 nvnetpslvt imentnnewt lccgvrhhfe lisssgstrv lhiegtpkph lvaalnlced
      841 eepelllcyn ntchfqkiwe esttptefdf nwnsvpaaia cafpyiiaft ndtmeirlii
      901 ngnlvhtiam pnlnlftskq diffsttape flpgkcerir ldtkpadkee pvsspspftq
      961 sssssrtnlq nnhgewkpir iyqiplqtls rstlsncsqr rcpspaepev vsapptsdks
     1021 flsvephral srscsssptp tptnlippri rk
//
LOCUS       XP_034188073            1052 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034188073
VERSION     XP_034188073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1052
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X1"
                     /calculated_mol_wt=118733
     Region          300..478
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          577..915
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..1052
                     /gene="LOC117607900"
                     /coded_by="XM_034332182.2:730..3888"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mmcldiklvs rlrgvsstss atnvsattps satstsstta flekckrgkg rrestgdass
       61 vtesdseeet lpigpemirr nrsrsvcvsa lssmhqghlr rrashhvstn dtisrrgvls
      121 rryygsveml pqieqdgydn grrfrvengd spgekeemfg spstpilenp eyqtrwyfky
      181 flgklhqnyi aadpernclf lsvvtnevsd qsvphyrvil wtksgaqkis lpysanktit
      241 irqilsnffg ldkldkapre vfapeiqkdl llleeqegsv nfkfgiiyak kgqttddeml
      301 snekgspdfd kfleilgeri rlknwdkyrg gldvkgdmtg kesyytvyag hevmyhvstm
      361 lpyskdnpqq lerkrhignd ivniiytddp naidtfnpnc irsqfthvfa vvstedngkg
      421 wrvaifcdes vplfgpslpc ppvfedpytl refllvklin gekatfntpt faqkrertld
      481 allrdmyqeh sqdsksnsml nrralsdvve apgrrreetr avemvqvgqa lkleaivrgl
      541 aptslatagp lkprpweprc iypdfpheiv cgdvwldnrl ilasengtfl ledsishrli
      601 fdesvqikql dvveqhgill frtgdkgkes nvyvfrlkef enntgnrsae vpndreedqd
      661 gedngededa eddgdesedn dcdnftrata kfkpvirrtr arvrplavrg rthikerklp
      721 rtrgcnlysi tmpggshlrm cvvvgrrltv lqwkhsaawt awcsaadtdt idgflfvkef
      781 nvnetpslvt imentnnewt lccgvrhhfe lisssgstrv lhiegtpkph lvaalnlced
      841 eepelllcyn ntchfqkiwe esttptefdf nwnsvpaaia cafpyiiaft ndtmeirlii
      901 ngnlvhtiam pnlnlftskq diffsttape flpgkcerir ldtkpadkee pvsspspftq
      961 sssssrtnlq nnhgewkpir iyqiplqtls rstlsncsqr rcpspaepev vsapptsdks
     1021 flsvephral srscsssptp tptnlippri rk
//
LOCUS       XP_034188083            1052 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034188083
VERSION     XP_034188083.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332192.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1052
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X1"
                     /calculated_mol_wt=118733
     Region          300..478
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          577..915
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..1052
                     /gene="LOC117607900"
                     /coded_by="XM_034332192.2:726..3884"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mmcldiklvs rlrgvsstss atnvsattps satstsstta flekckrgkg rrestgdass
       61 vtesdseeet lpigpemirr nrsrsvcvsa lssmhqghlr rrashhvstn dtisrrgvls
      121 rryygsveml pqieqdgydn grrfrvengd spgekeemfg spstpilenp eyqtrwyfky
      181 flgklhqnyi aadpernclf lsvvtnevsd qsvphyrvil wtksgaqkis lpysanktit
      241 irqilsnffg ldkldkapre vfapeiqkdl llleeqegsv nfkfgiiyak kgqttddeml
      301 snekgspdfd kfleilgeri rlknwdkyrg gldvkgdmtg kesyytvyag hevmyhvstm
      361 lpyskdnpqq lerkrhignd ivniiytddp naidtfnpnc irsqfthvfa vvstedngkg
      421 wrvaifcdes vplfgpslpc ppvfedpytl refllvklin gekatfntpt faqkrertld
      481 allrdmyqeh sqdsksnsml nrralsdvve apgrrreetr avemvqvgqa lkleaivrgl
      541 aptslatagp lkprpweprc iypdfpheiv cgdvwldnrl ilasengtfl ledsishrli
      601 fdesvqikql dvveqhgill frtgdkgkes nvyvfrlkef enntgnrsae vpndreedqd
      661 gedngededa eddgdesedn dcdnftrata kfkpvirrtr arvrplavrg rthikerklp
      721 rtrgcnlysi tmpggshlrm cvvvgrrltv lqwkhsaawt awcsaadtdt idgflfvkef
      781 nvnetpslvt imentnnewt lccgvrhhfe lisssgstrv lhiegtpkph lvaalnlced
      841 eepelllcyn ntchfqkiwe esttptefdf nwnsvpaaia cafpyiiaft ndtmeirlii
      901 ngnlvhtiam pnlnlftskq diffsttape flpgkcerir ldtkpadkee pvsspspftq
      961 sssssrtnlq nnhgewkpir iyqiplqtls rstlsncsqr rcpspaepev vsapptsdks
     1021 flsvephral srscsssptp tptnlippri rk
//
LOCUS       XP_034188110            1039 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034188110
VERSION     XP_034188110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1039
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X2"
                     /calculated_mol_wt=117262
     Region          300..478
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          564..902
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..1039
                     /gene="LOC117607900"
                     /coded_by="XM_034332219.2:1230..4349"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mmcldiklvs rlrgvsstss atnvsattps satstsstta flekckrgkg rrestgdass
       61 vtesdseeet lpigpemirr nrsrsvcvsa lssmhqghlr rrashhvstn dtisrrgvls
      121 rryygsveml pqieqdgydn grrfrvengd spgekeemfg spstpilenp eyqtrwyfky
      181 flgklhqnyi aadpernclf lsvvtnevsd qsvphyrvil wtksgaqkis lpysanktit
      241 irqilsnffg ldkldkapre vfapeiqkdl llleeqegsv nfkfgiiyak kgqttddeml
      301 snekgspdfd kfleilgeri rlknwdkyrg gldvkgdmtg kesyytvyag hevmyhvstm
      361 lpyskdnpqq lerkrhignd ivniiytddp naidtfnpnc irsqfthvfa vvstedngkg
      421 wrvaifcdes vplfgpslpc ppvfedpytl refllvklin gekatfntpt faqkrertld
      481 allrdmyqeh sqdsksnapg rrreetrave mvqvgqalkl eaivrglapt slatagplkp
      541 rpweprciyp dfpheivcgd vwldnrlila sengtflled sishrlifde svqikqldvv
      601 eqhgillfrt gdkgkesnvy vfrlkefenn tgnrsaevpn dreedqdged ngededaedd
      661 gdesedndcd nftratakfk pvirrtrarv rplavrgrth ikerklprtr gcnlysitmp
      721 ggshlrmcvv vgrrltvlqw khsaawtawc saadtdtidg flfvkefnvn etpslvtime
      781 ntnnewtlcc gvrhhfelis ssgstrvlhi egtpkphlva alnlcedeep elllcynntc
      841 hfqkiweest tptefdfnwn svpaaiacaf pyiiaftndt meirliingn lvhtiampnl
      901 nlftskqdif fsttapeflp gkcerirldt kpadkeepvs spspftqsss ssrtnlqnnh
      961 gewkpiriyq iplqtlsrst lsncsqrrcp spaepevvsa pptsdksfls vephralsrs
     1021 csssptptpt nlipprirk
//
LOCUS       XP_034188117            1015 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034188117
VERSION     XP_034188117.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332226.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1015
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X3"
                     /calculated_mol_wt=114958
     Region          263..441
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          540..878
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..1015
                     /gene="LOC117607900"
                     /coded_by="XM_034332226.2:1..3048"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 msslrrstnq strdgasgrr haltgtssks mrnvlhttta vpgsplrhrt kyelrsisle
       61 gtkvlpwtar stndtisrrg vlsrryygsv emlpqieqdg ydngrrfrve ngdspgekee
      121 mfgspstpil enpeyqtrwy fkyflgklhq nyiaadpern clflsvvtne vsdqsvphyr
      181 vilwtksgaq kislpysank titirqilsn ffgldkldka prevfapeiq kdlllleeqe
      241 gsvnfkfgii yakkgqttdd emlsnekgsp dfdkfleilg erirlknwdk yrggldvkgd
      301 mtgkesyytv yaghevmyhv stmlpyskdn pqqlerkrhi gndivniiyt ddpnaidtfn
      361 pncirsqfth vfavvstedn gkgwrvaifc desvplfgps lpcppvfedp ytlrefllvk
      421 lingekatfn tptfaqkrer tldallrdmy qehsqdsksn smlnrralsd vveapgrrre
      481 etravemvqv gqalkleaiv rglaptslat agplkprpwe prciypdfph eivcgdvwld
      541 nrlilaseng tflledsish rlifdesvqi kqldvveqhg illfrtgdkg kesnvyvfrl
      601 kefenntgnr saevpndree dqdgednged edaeddgdes edndcdnftr atakfkpvir
      661 rtrarvrpla vrgrthiker klprtrgcnl ysitmpggsh lrmcvvvgrr ltvlqwkhsa
      721 awtawcsaad tdtidgflfv kefnvnetps lvtimentnn ewtlccgvrh hfelisssgs
      781 trvlhiegtp kphlvaalnl cedeepelll cynntchfqk iweesttpte fdfnwnsvpa
      841 aiacafpyii aftndtmeir liingnlvht iampnlnlft skqdiffstt apeflpgkce
      901 rirldtkpad keepvsspsp ftqsssssrt nlqnnhgewk piriyqiplq tlsrstlsnc
      961 sqrrcpspae pevvsappts dksflsveph ralsrscsss ptptptnlip prirk
//
LOCUS       XP_034188122             924 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034188122
VERSION     XP_034188122.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332231.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..924
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X4"
                     /calculated_mol_wt=104828
     Region          172..350
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          449..787
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..924
                     /gene="LOC117607900"
                     /coded_by="XM_034332231.2:488..3262"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mlpqieqdgy dngrrfrven gdspgekeem fgspstpile npeyqtrwyf kyflgklhqn
       61 yiaadpernc lflsvvtnev sdqsvphyrv ilwtksgaqk islpysankt itirqilsnf
      121 fgldkldkap revfapeiqk dlllleeqeg svnfkfgiiy akkgqttdde mlsnekgspd
      181 fdkfleilge rirlknwdky rggldvkgdm tgkesyytvy aghevmyhvs tmlpyskdnp
      241 qqlerkrhig ndivniiytd dpnaidtfnp ncirsqfthv favvstedng kgwrvaifcd
      301 esvplfgpsl pcppvfedpy tlrefllvkl ingekatfnt ptfaqkrert ldallrdmyq
      361 ehsqdsksns mlnrralsdv veapgrrree travemvqvg qalkleaivr glaptslata
      421 gplkprpwep rciypdfphe ivcgdvwldn rlilasengt flledsishr lifdesvqik
      481 qldvveqhgi llfrtgdkgk esnvyvfrlk efenntgnrs aevpndreed qdgedngede
      541 daeddgdese dndcdnftra takfkpvirr trarvrplav rgrthikerk lprtrgcnly
      601 sitmpggshl rmcvvvgrrl tvlqwkhsaa wtawcsaadt dtidgflfvk efnvnetpsl
      661 vtimentnne wtlccgvrhh felisssgst rvlhiegtpk phlvaalnlc edeepelllc
      721 ynntchfqki weesttptef dfnwnsvpaa iacafpyiia ftndtmeirl iingnlvhti
      781 ampnlnlfts kqdiffstta peflpgkcer irldtkpadk eepvsspspf tqsssssrtn
      841 lqnnhgewkp iriyqiplqt lsrstlsncs qrrcpspaep evvsapptsd ksflsvephr
      901 alsrscsssp tptptnlipp rirk
//
LOCUS       XP_034188133             924 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase-activating Rap/Ran-GAP domain-like protein 3 isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034188133
VERSION     XP_034188133.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332242.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..924
                     /product="GTPase-activating Rap/Ran-GAP domain-like
                     protein 3 isoform X4"
                     /calculated_mol_wt=104828
     Region          172..350
                     /region_name="Rap_GAP"
                     /note="Rap/ran-GAP; pfam02145"
                     /db_xref="CDD:460463"
     Region          449..787
                     /region_name="CNH"
                     /note="CNH domain; cl02434"
                     /db_xref="CDD:470577"
     CDS             1..924
                     /gene="LOC117607900"
                     /coded_by="XM_034332242.2:484..3258"
                     /db_xref="GeneID:117607900"
ORIGIN      
        1 mlpqieqdgy dngrrfrven gdspgekeem fgspstpile npeyqtrwyf kyflgklhqn
       61 yiaadpernc lflsvvtnev sdqsvphyrv ilwtksgaqk islpysankt itirqilsnf
      121 fgldkldkap revfapeiqk dlllleeqeg svnfkfgiiy akkgqttdde mlsnekgspd
      181 fdkfleilge rirlknwdky rggldvkgdm tgkesyytvy aghevmyhvs tmlpyskdnp
      241 qqlerkrhig ndivniiytd dpnaidtfnp ncirsqfthv favvstedng kgwrvaifcd
      301 esvplfgpsl pcppvfedpy tlrefllvkl ingekatfnt ptfaqkrert ldallrdmyq
      361 ehsqdsksns mlnrralsdv veapgrrree travemvqvg qalkleaivr glaptslata
      421 gplkprpwep rciypdfphe ivcgdvwldn rlilasengt flledsishr lifdesvqik
      481 qldvveqhgi llfrtgdkgk esnvyvfrlk efenntgnrs aevpndreed qdgedngede
      541 daeddgdese dndcdnftra takfkpvirr trarvrplav rgrthikerk lprtrgcnly
      601 sitmpggshl rmcvvvgrrl tvlqwkhsaa wtawcsaadt dtidgflfvk efnvnetpsl
      661 vtimentnne wtlccgvrhh felisssgst rvlhiegtpk phlvaalnlc edeepelllc
      721 ynntchfqki weesttptef dfnwnsvpaa iacafpyiia ftndtmeirl iingnlvhti
      781 ampnlnlfts kqdiffstta peflpgkcer irldtkpadk eepvsspspf tqsssssrtn
      841 lqnnhgewkp iriyqiplqt lsrstlsncs qrrcpspaep evvsapptsd ksflsvephr
      901 alsrscsssp tptptnlipp rirk
//
LOCUS       XP_034188155             982 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear hormone receptor FTZ-F1 beta [Osmia lignaria lignaria].
ACCESSION   XP_034188155
VERSION     XP_034188155.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332264.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188155.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..982
                     /product="nuclear hormone receptor FTZ-F1 beta"
                     /calculated_mol_wt=105188
     Region          540..632
                     /region_name="NR_DBD_Lrh-1_like"
                     /note="The DNA-binding domain of Lrh-1 like nuclear
                     receptor family like is composed of two C4-type zinc
                     fingers; cd07167"
                     /db_xref="CDD:143541"
     Site            order(540,543,557,560,576,582,592,595)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143541"
     Site            order(550..552,558..559,561,563,565..566,569,589..590,596,
                     609..611,614..620,626)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143541"
     Region          789..951
                     /region_name="NR_LBD_F2"
                     /note="Ligand-binding domain of nuclear receptor family 2;
                     cd06930"
                     /db_xref="CDD:132728"
     Site            order(789,792..793,796,799..800,829..830,833..834,837,840,
                     850,873)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132728"
     Site            order(804,808,818..819,821..822,825..826)
                     /site_type="other"
                     /note="coactivator recognition site [polypeptide binding]"
                     /db_xref="CDD:132728"
     CDS             1..982
                     /gene="Hr39"
                     /coded_by="XM_034332264.2:331..3279"
                     /db_xref="GeneID:117607951"
ORIGIN      
        1 mseqngpseg pgpgpgpgpg pgwwpasqsw ktnsctsvss ssttstapds sasvsvscti
       61 stssltttts tssstsccss tpsgpsctit aarssetgkn vsvttinvpn nqdihdgkgi
      121 ckyltgqngv tvsvvsscgs vldcgnanvv sttgigissg shsigigigv nvlqsqntgd
      181 ddaedsdvei skidfrgvnl rtkkkrdvpv nqagekigdv daedgnccyd gndisqqqpe
      241 rpmswegels dqemssntit nqdthedtsm egvqvcsasp gpmeqkfpik pepdfrsspg
      301 fafgsfhdtg lplthgqqmq qqqqqrnien leqtqqndlp llvgkllggy nsstpnhspv
      361 lnprhhltkh shtrsqvpsp dsaihsaysv fssptqspha arhsalgags pvpssslsls
      421 rhsfnnstss lslslshsls rnnsdasssc ysygslsppt hspvqqprhp qhhqhqvaqg
      481 splhlpatap saiahhysss tpgselspeg htapddqedc ripsapssis trqqlinspc
      541 picgdkisgf hygifscesc kgffkrtvqn rknyvclrga gcpvtvatrk kcpacrfdkc
      601 lnmgmkleai redrtrggrs tyqctytlpa slvsnsasgm tgdklttggn cspappgseh
      661 hyssrhhshk mqmvpqllqd imdvehlwhy ndndrmsgiq aggtnatrnn damllgvgtg
      721 ggsgtgsdag srverstngt agngnsnnrr ddrpnvsnvn seqratsvns ssqignntng
      781 nstqhpdfls nlcniadhrl ykivkwcksl plfknisidd qicllinswc elllfsccfr
      841 smstpgeirv slgksitleq arqlglatci ermlaftnnl rrlrvdqyey vamkvivllt
      901 sdtselkepe kvrasqekal qalqqytiar ypempakfge lllripdlqr tcqagkellt
      961 akraegegss fnllmellrg dh
//
LOCUS       XP_034184742            1126 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate ionotropic receptor NMDA type subunit 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184742
VERSION     XP_034184742.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328851.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1126
                     /product="glutamate ionotropic receptor NMDA type subunit
                     2 isoform X1"
                     /calculated_mol_wt=127088
     Region          99..480
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          497..908
                     /region_name="PBP2_iGluR_NMDA_Nr2"
                     /note="The ligand-binding domain of the NR2 subunit of
                     ionotropic NMDA (N-methyl-D-aspartate) glutamate
                     receptors, a member of the type 2 periplasmic binding fold
                     protein superfamily; cd13718"
                     /db_xref="CDD:270436"
     Site            order(612..615,617,619..621,774,796..797,801,839,862..864,
                     867,873,889..892,895)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270436"
     Site            order(612..614,619,796..798,838..839)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270436"
     CDS             1..1126
                     /gene="Nmdar2"
                     /coded_by="XM_034328851.2:907..4287"
                     /db_xref="GeneID:117606431"
ORIGIN      
        1 mrsflyalls llsvvcsfqs ggvtrggsgi qigtsdrgth skgtgwggrg hggvklgegr
       61 fpnstrqfrt ttttlppppp rsthaphssd anqynitylr vgmmvphmsf kvreytkavt
      121 savhslqkst rspklklfer ydihvkvamk amtpsptail dslceeflpm nvsailylmn
      181 yeqygrstas aqyflqlagy lgipviawna dnsglerras qsnlqlqlap slehqtaaml
      241 silerykwhq fsvvtsqiag hddfvqavre risdmqdtfk ftilsailvt ephdllelvn
      301 sesrvmllys treeathilt aahdykitge nyvwvvtqsv ienlhtpskf pvgmlgvhfd
      361 tsteslvsei ttavkvyayg vedflkdenn qhlslntqls ckesfgesrw ntgdlffkyl
      421 knvsvegdqg kpnveftqdg vlksaelkim nlrpgvskql vweeigvwks wekegldikd
      481 ivwpgnthtp pqgvpekfyl kitfleeppy inlappdpvs gkclmdrgvh crvakesdmv
      541 evdiqsaqkn esfyqccsgf cidllqkfse eigftyelvr vedgkwgtle ngkwngliad
      601 lvnrktdmvm tslminsere avvdftvpym etgiaivvak rtgiisptaf lepfdtaswm
      661 lvgivaihaa timillfewl spsgfnmkvn psgaaqilkn pspnhrfsfc rtywlvwavl
      721 fqaavhidsp rgftarfmtn vwalfavvfl aiytanlaaf mitreefhef tgvddhrlak
      781 pwshkpmfkf gtipwshtds tlakyfkemh aymkkfnkss vaegieavis gemdafiydg
      841 tvldylvaqd edcrlltvgs wyamtgygla fsrnskylqm fnkrlldyrd ngdlerlrry
      901 wmtgtckpgk evqkssdpla leqflsafll lmmgillaaa flllehlyfk yvrqhlarsd
      961 gggccalfsl smgksltfrg avyeaqdilr yhrcrdpicd thlwkvkhel dmaririrql
     1021 ekdleahgik pshakrktgs lgwwrgcfqs sdrhsqpesl aveaphplpp yfdsfidaid
     1081 varprdmtrn hvvsteysqk flpteiyrip ddgaarteia emetvl
//
LOCUS       XP_076546398            1126 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate ionotropic receptor NMDA type subunit 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546398
VERSION     XP_076546398.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690283.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1126
                     /product="glutamate ionotropic receptor NMDA type subunit
                     2 isoform X1"
                     /calculated_mol_wt=127088
     Region          99..480
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          497..908
                     /region_name="PBP2_iGluR_NMDA_Nr2"
                     /note="The ligand-binding domain of the NR2 subunit of
                     ionotropic NMDA (N-methyl-D-aspartate) glutamate
                     receptors, a member of the type 2 periplasmic binding fold
                     protein superfamily; cd13718"
                     /db_xref="CDD:270436"
     Site            order(612..615,617,619..621,774,796..797,801,839,862..864,
                     867,873,889..892,895)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270436"
     Site            order(612..614,619,796..798,838..839)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270436"
     CDS             1..1126
                     /gene="Nmdar2"
                     /coded_by="XM_076690283.1:766..4146"
                     /db_xref="GeneID:117606431"
ORIGIN      
        1 mrsflyalls llsvvcsfqs ggvtrggsgi qigtsdrgth skgtgwggrg hggvklgegr
       61 fpnstrqfrt ttttlppppp rsthaphssd anqynitylr vgmmvphmsf kvreytkavt
      121 savhslqkst rspklklfer ydihvkvamk amtpsptail dslceeflpm nvsailylmn
      181 yeqygrstas aqyflqlagy lgipviawna dnsglerras qsnlqlqlap slehqtaaml
      241 silerykwhq fsvvtsqiag hddfvqavre risdmqdtfk ftilsailvt ephdllelvn
      301 sesrvmllys treeathilt aahdykitge nyvwvvtqsv ienlhtpskf pvgmlgvhfd
      361 tsteslvsei ttavkvyayg vedflkdenn qhlslntqls ckesfgesrw ntgdlffkyl
      421 knvsvegdqg kpnveftqdg vlksaelkim nlrpgvskql vweeigvwks wekegldikd
      481 ivwpgnthtp pqgvpekfyl kitfleeppy inlappdpvs gkclmdrgvh crvakesdmv
      541 evdiqsaqkn esfyqccsgf cidllqkfse eigftyelvr vedgkwgtle ngkwngliad
      601 lvnrktdmvm tslminsere avvdftvpym etgiaivvak rtgiisptaf lepfdtaswm
      661 lvgivaihaa timillfewl spsgfnmkvn psgaaqilkn pspnhrfsfc rtywlvwavl
      721 fqaavhidsp rgftarfmtn vwalfavvfl aiytanlaaf mitreefhef tgvddhrlak
      781 pwshkpmfkf gtipwshtds tlakyfkemh aymkkfnkss vaegieavis gemdafiydg
      841 tvldylvaqd edcrlltvgs wyamtgygla fsrnskylqm fnkrlldyrd ngdlerlrry
      901 wmtgtckpgk evqkssdpla leqflsafll lmmgillaaa flllehlyfk yvrqhlarsd
      961 gggccalfsl smgksltfrg avyeaqdilr yhrcrdpicd thlwkvkhel dmaririrql
     1021 ekdleahgik pshakrktgs lgwwrgcfqs sdrhsqpesl aveaphplpp yfdsfidaid
     1081 varprdmtrn hvvsteysqk flpteiyrip ddgaarteia emetvl
//
LOCUS       XP_034184751            1126 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate ionotropic receptor NMDA type subunit 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184751
VERSION     XP_034184751.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328860.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1126
                     /product="glutamate ionotropic receptor NMDA type subunit
                     2 isoform X1"
                     /calculated_mol_wt=127088
     Region          99..480
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          497..908
                     /region_name="PBP2_iGluR_NMDA_Nr2"
                     /note="The ligand-binding domain of the NR2 subunit of
                     ionotropic NMDA (N-methyl-D-aspartate) glutamate
                     receptors, a member of the type 2 periplasmic binding fold
                     protein superfamily; cd13718"
                     /db_xref="CDD:270436"
     Site            order(612..615,617,619..621,774,796..797,801,839,862..864,
                     867,873,889..892,895)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270436"
     Site            order(612..614,619,796..798,838..839)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270436"
     CDS             1..1126
                     /gene="Nmdar2"
                     /coded_by="XM_034328860.2:497..3877"
                     /db_xref="GeneID:117606431"
ORIGIN      
        1 mrsflyalls llsvvcsfqs ggvtrggsgi qigtsdrgth skgtgwggrg hggvklgegr
       61 fpnstrqfrt ttttlppppp rsthaphssd anqynitylr vgmmvphmsf kvreytkavt
      121 savhslqkst rspklklfer ydihvkvamk amtpsptail dslceeflpm nvsailylmn
      181 yeqygrstas aqyflqlagy lgipviawna dnsglerras qsnlqlqlap slehqtaaml
      241 silerykwhq fsvvtsqiag hddfvqavre risdmqdtfk ftilsailvt ephdllelvn
      301 sesrvmllys treeathilt aahdykitge nyvwvvtqsv ienlhtpskf pvgmlgvhfd
      361 tsteslvsei ttavkvyayg vedflkdenn qhlslntqls ckesfgesrw ntgdlffkyl
      421 knvsvegdqg kpnveftqdg vlksaelkim nlrpgvskql vweeigvwks wekegldikd
      481 ivwpgnthtp pqgvpekfyl kitfleeppy inlappdpvs gkclmdrgvh crvakesdmv
      541 evdiqsaqkn esfyqccsgf cidllqkfse eigftyelvr vedgkwgtle ngkwngliad
      601 lvnrktdmvm tslminsere avvdftvpym etgiaivvak rtgiisptaf lepfdtaswm
      661 lvgivaihaa timillfewl spsgfnmkvn psgaaqilkn pspnhrfsfc rtywlvwavl
      721 fqaavhidsp rgftarfmtn vwalfavvfl aiytanlaaf mitreefhef tgvddhrlak
      781 pwshkpmfkf gtipwshtds tlakyfkemh aymkkfnkss vaegieavis gemdafiydg
      841 tvldylvaqd edcrlltvgs wyamtgygla fsrnskylqm fnkrlldyrd ngdlerlrry
      901 wmtgtckpgk evqkssdpla leqflsafll lmmgillaaa flllehlyfk yvrqhlarsd
      961 gggccalfsl smgksltfrg avyeaqdilr yhrcrdpicd thlwkvkhel dmaririrql
     1021 ekdleahgik pshakrktgs lgwwrgcfqs sdrhsqpesl aveaphplpp yfdsfidaid
     1081 varprdmtrn hvvsteysqk flpteiyrip ddgaarteia emetvl
//
LOCUS       XP_034184761            1116 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate ionotropic receptor NMDA type subunit 2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184761
VERSION     XP_034184761.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328870.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1116
                     /product="glutamate ionotropic receptor NMDA type subunit
                     2 isoform X2"
                     /calculated_mol_wt=126137
     Region          99..480
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          497..898
                     /region_name="PBP2_iGluR_NMDA_Nr2"
                     /note="The ligand-binding domain of the NR2 subunit of
                     ionotropic NMDA (N-methyl-D-aspartate) glutamate
                     receptors, a member of the type 2 periplasmic binding fold
                     protein superfamily; cd13718"
                     /db_xref="CDD:270436"
     Site            order(612..615,617,619..621,764,786..787,791,829,852..854,
                     857,863,879..882,885)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270436"
     Site            order(612..614,619,786..788,828..829)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270436"
     CDS             1..1116
                     /gene="Nmdar2"
                     /coded_by="XM_034328870.2:907..4257"
                     /db_xref="GeneID:117606431"
ORIGIN      
        1 mrsflyalls llsvvcsfqs ggvtrggsgi qigtsdrgth skgtgwggrg hggvklgegr
       61 fpnstrqfrt ttttlppppp rsthaphssd anqynitylr vgmmvphmsf kvreytkavt
      121 savhslqkst rspklklfer ydihvkvamk amtpsptail dslceeflpm nvsailylmn
      181 yeqygrstas aqyflqlagy lgipviawna dnsglerras qsnlqlqlap slehqtaaml
      241 silerykwhq fsvvtsqiag hddfvqavre risdmqdtfk ftilsailvt ephdllelvn
      301 sesrvmllys treeathilt aahdykitge nyvwvvtqsv ienlhtpskf pvgmlgvhfd
      361 tsteslvsei ttavkvyayg vedflkdenn qhlslntqls ckesfgesrw ntgdlffkyl
      421 knvsvegdqg kpnveftqdg vlksaelkim nlrpgvskql vweeigvwks wekegldikd
      481 ivwpgnthtp pqgvpekfyl kitfleeppy inlappdpvs gkclmdrgvh crvakesdmv
      541 evdiqsaqkn esfyqccsgf cidllqkfse eigftyelvr vedgkwgtle ngkwngliad
      601 lvnrktdmvm tslminsere avvdftvpym etgiaivvak rtgiisptaf lepfdtaswm
      661 lvgivaihaa timillfewl spsgfnmkkn pspnhrfsfc rtywlvwavl fqaavhidsp
      721 rgftarfmtn vwalfavvfl aiytanlaaf mitreefhef tgvddhrlak pwshkpmfkf
      781 gtipwshtds tlakyfkemh aymkkfnkss vaegieavis gemdafiydg tvldylvaqd
      841 edcrlltvgs wyamtgygla fsrnskylqm fnkrlldyrd ngdlerlrry wmtgtckpgk
      901 evqkssdpla leqflsafll lmmgillaaa flllehlyfk yvrqhlarsd gggccalfsl
      961 smgksltfrg avyeaqdilr yhrcrdpicd thlwkvkhel dmaririrql ekdleahgik
     1021 pshakrktgs lgwwrgcfqs sdrhsqpesl aveaphplpp yfdsfidaid varprdmtrn
     1081 hvvsteysqk flpteiyrip ddgaarteia emetvl
//
LOCUS       XP_034184770            1087 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate ionotropic receptor NMDA type subunit 2 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184770
VERSION     XP_034184770.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328879.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1087
                     /product="glutamate ionotropic receptor NMDA type subunit
                     2 isoform X3"
                     /calculated_mol_wt=122678
     Region          99..480
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          497..908
                     /region_name="PBP2_iGluR_NMDA_Nr2"
                     /note="The ligand-binding domain of the NR2 subunit of
                     ionotropic NMDA (N-methyl-D-aspartate) glutamate
                     receptors, a member of the type 2 periplasmic binding fold
                     protein superfamily; cd13718"
                     /db_xref="CDD:270436"
     Site            order(612..615,617,619..621,774,796..797,801,839,862..864,
                     867,873,889..892,895)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270436"
     Site            order(612..614,619,796..798,838..839)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270436"
     CDS             1..1087
                     /gene="Nmdar2"
                     /coded_by="XM_034328879.2:907..4170"
                     /db_xref="GeneID:117606431"
ORIGIN      
        1 mrsflyalls llsvvcsfqs ggvtrggsgi qigtsdrgth skgtgwggrg hggvklgegr
       61 fpnstrqfrt ttttlppppp rsthaphssd anqynitylr vgmmvphmsf kvreytkavt
      121 savhslqkst rspklklfer ydihvkvamk amtpsptail dslceeflpm nvsailylmn
      181 yeqygrstas aqyflqlagy lgipviawna dnsglerras qsnlqlqlap slehqtaaml
      241 silerykwhq fsvvtsqiag hddfvqavre risdmqdtfk ftilsailvt ephdllelvn
      301 sesrvmllys treeathilt aahdykitge nyvwvvtqsv ienlhtpskf pvgmlgvhfd
      361 tsteslvsei ttavkvyayg vedflkdenn qhlslntqls ckesfgesrw ntgdlffkyl
      421 knvsvegdqg kpnveftqdg vlksaelkim nlrpgvskql vweeigvwks wekegldikd
      481 ivwpgnthtp pqgvpekfyl kitfleeppy inlappdpvs gkclmdrgvh crvakesdmv
      541 evdiqsaqkn esfyqccsgf cidllqkfse eigftyelvr vedgkwgtle ngkwngliad
      601 lvnrktdmvm tslminsere avvdftvpym etgiaivvak rtgiisptaf lepfdtaswm
      661 lvgivaihaa timillfewl spsgfnmkvn psgaaqilkn pspnhrfsfc rtywlvwavl
      721 fqaavhidsp rgftarfmtn vwalfavvfl aiytanlaaf mitreefhef tgvddhrlak
      781 pwshkpmfkf gtipwshtds tlakyfkemh aymkkfnkss vaegieavis gemdafiydg
      841 tvldylvaqd edcrlltvgs wyamtgygla fsrnskylqm fnkrlldyrd ngdlerlrry
      901 wmtgtckpgk evqkssdpla leqflsafll lmmgillaaa flllehlyfk yvrqhlarsd
      961 gggccalfsl smgksltfrg avyeaqdilr yhrcrdpicd thlwkvkhel dmaririrql
     1021 ekdleahgik pshakfidai dvarprdmtr nhvvsteysq kflpteiyri pddgaartei
     1081 aemetvl
//
LOCUS       XP_034184780            1077 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate ionotropic receptor NMDA type subunit 2 isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034184780
VERSION     XP_034184780.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328889.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1077
                     /product="glutamate ionotropic receptor NMDA type subunit
                     2 isoform X4"
                     /calculated_mol_wt=121727
     Region          99..480
                     /region_name="Periplasmic_Binding_Protein_type1"
                     /note="Type 1 periplasmic binding fold superfamily;
                     cl10011"
                     /db_xref="CDD:471960"
     Region          497..898
                     /region_name="PBP2_iGluR_NMDA_Nr2"
                     /note="The ligand-binding domain of the NR2 subunit of
                     ionotropic NMDA (N-methyl-D-aspartate) glutamate
                     receptors, a member of the type 2 periplasmic binding fold
                     protein superfamily; cd13718"
                     /db_xref="CDD:270436"
     Site            order(612..615,617,619..621,764,786..787,791,829,852..854,
                     857,863,879..882,885)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270436"
     Site            order(612..614,619,786..788,828..829)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270436"
     CDS             1..1077
                     /gene="Nmdar2"
                     /coded_by="XM_034328889.2:907..4140"
                     /db_xref="GeneID:117606431"
ORIGIN      
        1 mrsflyalls llsvvcsfqs ggvtrggsgi qigtsdrgth skgtgwggrg hggvklgegr
       61 fpnstrqfrt ttttlppppp rsthaphssd anqynitylr vgmmvphmsf kvreytkavt
      121 savhslqkst rspklklfer ydihvkvamk amtpsptail dslceeflpm nvsailylmn
      181 yeqygrstas aqyflqlagy lgipviawna dnsglerras qsnlqlqlap slehqtaaml
      241 silerykwhq fsvvtsqiag hddfvqavre risdmqdtfk ftilsailvt ephdllelvn
      301 sesrvmllys treeathilt aahdykitge nyvwvvtqsv ienlhtpskf pvgmlgvhfd
      361 tsteslvsei ttavkvyayg vedflkdenn qhlslntqls ckesfgesrw ntgdlffkyl
      421 knvsvegdqg kpnveftqdg vlksaelkim nlrpgvskql vweeigvwks wekegldikd
      481 ivwpgnthtp pqgvpekfyl kitfleeppy inlappdpvs gkclmdrgvh crvakesdmv
      541 evdiqsaqkn esfyqccsgf cidllqkfse eigftyelvr vedgkwgtle ngkwngliad
      601 lvnrktdmvm tslminsere avvdftvpym etgiaivvak rtgiisptaf lepfdtaswm
      661 lvgivaihaa timillfewl spsgfnmkkn pspnhrfsfc rtywlvwavl fqaavhidsp
      721 rgftarfmtn vwalfavvfl aiytanlaaf mitreefhef tgvddhrlak pwshkpmfkf
      781 gtipwshtds tlakyfkemh aymkkfnkss vaegieavis gemdafiydg tvldylvaqd
      841 edcrlltvgs wyamtgygla fsrnskylqm fnkrlldyrd ngdlerlrry wmtgtckpgk
      901 evqkssdpla leqflsafll lmmgillaaa flllehlyfk yvrqhlarsd gggccalfsl
      961 smgksltfrg avyeaqdilr yhrcrdpicd thlwkvkhel dmaririrql ekdleahgik
     1021 pshakfidai dvarprdmtr nhvvsteysq kflpteiyri pddgaartei aemetvl
//
LOCUS       XP_034184805             571 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein klumpfuss isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034184805
VERSION     XP_034184805.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328914.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..571
                     /product="zinc finger protein klumpfuss isoform X1"
                     /calculated_mol_wt=61939
     Region          385..>443
                     /region_name="lambda-1"
                     /note="inner capsid protein lambda-1 or VP3; cd11674"
                     /db_xref="CDD:212564"
     Region          421..442
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(421,424,437,442)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:212564"
     Region          457..477
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(462,464,466,468..469,472..473,476,490,492,496..497,
                     500..501,504,518,520,522,524..525,528..529,532)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          469..494
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          485..505
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          497..519
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          511..533
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          513..533
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..571
                     /gene="klu"
                     /coded_by="XM_034328914.2:313..2028"
                     /db_xref="GeneID:117606459"
ORIGIN      
        1 mveltysaen qertikedev ggstvvaegv vggikgpggg crassmtmae savedaaaap
       61 pgpakpppps ctnnntlaat anrnhrnlrr rkrldqvldi lqhrsspseg evlrfegsgp
      121 aseeeedvfg sprsssrsaa dppsgisllp lrlkseepvt pneeeanans nsnanegngd
      181 qqqqnhhshy phhpshhhrh rhrhhqrnna nhlydhlhpp hprssapkya gctcvqcsqs
      241 atppmmlpsp tspltpltpl tpltpltlpp ltpgslssys fehymhtkyl pdifrgrshs
      301 dsdlvpgwdq kpplssssmf lnqpmrsgsi esdatspqes pldlsmknlm asaaaaaafg
      361 rppalqllpp givsrgsvsv pvvkgdvasp ttkesvavry nlevspvvee mppgadvayv
      421 cpvcgqmfsl hdrlakhmas rhrrqgpqda sakaylcdvc krsfarsdml trhmrlhtgv
      481 kpytckvcgq vfsrsdhlst hqrthtgekp ykcpqcayaa crrdmitrhl rtharfpdvq
      541 tpksepglla gedspmftqe msspsatika e
//
LOCUS       XP_076546399             526 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein klumpfuss isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546399
VERSION     XP_076546399.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690284.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..526
                     /product="zinc finger protein klumpfuss isoform X2"
                     /calculated_mol_wt=57431
     Region          340..>398
                     /region_name="lambda-1"
                     /note="inner capsid protein lambda-1 or VP3; cd11674"
                     /db_xref="CDD:212564"
     Region          376..397
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(376,379,392,397)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:212564"
     Region          412..432
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(417,419,421,423..424,427..428,431,445,447,451..452,
                     455..456,459,473,475,477,479..480,483..484,487)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          424..449
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          440..460
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          452..474
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          466..488
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          468..488
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..526
                     /gene="klu"
                     /coded_by="XM_076690284.1:1288..2868"
                     /db_xref="GeneID:117606459"
ORIGIN      
        1 mtmaesaved aaaappgpak ppppsctnnn tlaatanrnh rnlrrrkrld qvldilqhrs
       61 spsegevlrf egsgpaseee edvfgsprss srsaadppsg isllplrlks eepvtpneee
      121 anansnsnan egngdqqqqn hhshyphhps hhhrhrhrhh qrnnanhlyd hlhpphprss
      181 apkyagctcv qcsqsatppm mlpsptsplt pltpltpltp ltlppltpgs lssysfehym
      241 htkylpdifr grshsdsdlv pgwdqkppls sssmflnqpm rsgsiesdat spqespldls
      301 mknlmasaaa aaafgrppal qllppgivsr gsvsvpvvkg dvaspttkes vavrynlevs
      361 pvveemppga dvayvcpvcg qmfslhdrla khmasrhrrq gpqdasakay lcdvckrsfa
      421 rsdmltrhmr lhtgvkpytc kvcgqvfsrs dhlsthqrth tgekpykcpq cayaacrrdm
      481 itrhlrthar fpdvqtpkse pgllagedsp mftqemssps atikae
//
LOCUS       XP_076546400             526 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein klumpfuss isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546400
VERSION     XP_076546400.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690285.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..526
                     /product="zinc finger protein klumpfuss isoform X2"
                     /calculated_mol_wt=57431
     Region          340..>398
                     /region_name="lambda-1"
                     /note="inner capsid protein lambda-1 or VP3; cd11674"
                     /db_xref="CDD:212564"
     Region          376..397
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(376,379,392,397)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:212564"
     Region          412..432
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(417,419,421,423..424,427..428,431,445,447,451..452,
                     455..456,459,473,475,477,479..480,483..484,487)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          424..449
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          440..460
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          452..474
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          466..488
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          468..488
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..526
                     /gene="klu"
                     /coded_by="XM_076690285.1:1352..2932"
                     /db_xref="GeneID:117606459"
ORIGIN      
        1 mtmaesaved aaaappgpak ppppsctnnn tlaatanrnh rnlrrrkrld qvldilqhrs
       61 spsegevlrf egsgpaseee edvfgsprss srsaadppsg isllplrlks eepvtpneee
      121 anansnsnan egngdqqqqn hhshyphhps hhhrhrhrhh qrnnanhlyd hlhpphprss
      181 apkyagctcv qcsqsatppm mlpsptsplt pltpltpltp ltlppltpgs lssysfehym
      241 htkylpdifr grshsdsdlv pgwdqkppls sssmflnqpm rsgsiesdat spqespldls
      301 mknlmasaaa aaafgrppal qllppgivsr gsvsvpvvkg dvaspttkes vavrynlevs
      361 pvveemppga dvayvcpvcg qmfslhdrla khmasrhrrq gpqdasakay lcdvckrsfa
      421 rsdmltrhmr lhtgvkpytc kvcgqvfsrs dhlsthqrth tgekpykcpq cayaacrrdm
      481 itrhlrthar fpdvqtpkse pgllagedsp mftqemssps atikae
//
LOCUS       XP_034184820             508 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606470 [Osmia lignaria lignaria].
ACCESSION   XP_034184820
VERSION     XP_034184820.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328929.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="uncharacterized protein LOC117606470"
                     /calculated_mol_wt=59561
     Region          <1..>270
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..508
                     /gene="LOC117606470"
                     /coded_by="XM_034328929.2:128..1654"
                     /db_xref="GeneID:117606470"
ORIGIN      
        1 mgrdqrylee qlqrresevs elrrnhrele akffqleraf qvnlelqnrq evefnrvkle
       61 nqelreinde tlrvrtelns rlmnatiekd hwknaflqqk elinsnnier nntvaevkre
      121 cedilkttre taekqfqelv elyngtkekl trseeeigty essrqeyenr tfelanllet
      181 lkrfevdvss vcqlvaealk nlteqgnvye esmknlrhlv wttkdrkdep elillrkqns
      241 vlrelvkslk rkfqvlqeth netakraneq rtevtshdie vnlqnceenl pkqmtldkey
      301 asntenhrns liknnnhnfn tendaktrep tkalenynrr sefdiessti dksgrllhie
      361 shtngvlcee yilklskdre ikikypvfln nseipvkief vddeneyekt lsdgmvilfk
      421 hiyafvnvkq tgipcstqta ktktinnfaq tamtginsfs rlnvgatvps ilipiqrarh
      481 tsrgtriypr hrtprvhgyl asravtqg
//
LOCUS       XP_034184830             508 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606470 [Osmia lignaria lignaria].
ACCESSION   XP_034184830
VERSION     XP_034184830.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328939.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="uncharacterized protein LOC117606470"
                     /calculated_mol_wt=59561
     Region          <1..>270
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..508
                     /gene="LOC117606470"
                     /coded_by="XM_034328939.2:241..1767"
                     /db_xref="GeneID:117606470"
ORIGIN      
        1 mgrdqrylee qlqrresevs elrrnhrele akffqleraf qvnlelqnrq evefnrvkle
       61 nqelreinde tlrvrtelns rlmnatiekd hwknaflqqk elinsnnier nntvaevkre
      121 cedilkttre taekqfqelv elyngtkekl trseeeigty essrqeyenr tfelanllet
      181 lkrfevdvss vcqlvaealk nlteqgnvye esmknlrhlv wttkdrkdep elillrkqns
      241 vlrelvkslk rkfqvlqeth netakraneq rtevtshdie vnlqnceenl pkqmtldkey
      301 asntenhrns liknnnhnfn tendaktrep tkalenynrr sefdiessti dksgrllhie
      361 shtngvlcee yilklskdre ikikypvfln nseipvkief vddeneyekt lsdgmvilfk
      421 hiyafvnvkq tgipcstqta ktktinnfaq tamtginsfs rlnvgatvps ilipiqrarh
      481 tsrgtriypr hrtprvhgyl asravtqg
//
LOCUS       XP_034184886             106 aa            linear   INV 03-SEP-2025
DEFINITION  MAPK regulated corepressor interacting protein 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034184886
VERSION     XP_034184886.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328995.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="MAPK regulated corepressor interacting protein
                     2"
                     /calculated_mol_wt=12206
     Region          4..101
                     /region_name="FAM195"
                     /note="FAM195 family; pfam14799"
                     /db_xref="CDD:464321"
     CDS             1..106
                     /gene="LOC117606491"
                     /coded_by="XM_034328995.2:205..525"
                     /db_xref="GeneID:117606491"
ORIGIN      
        1 msgrsqmtam ngkrpssips rhqsetvaqh fdlikyiyds wntvsreldm chnqphsnss
       61 nyrngasvty yqerepnpql kdfepfnlea wwgqrvvqsi trnans
//
LOCUS       XP_076546401            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546401
VERSION     XP_076546401.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690286.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_076690286.1:1229..9397"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_076546402            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546402
VERSION     XP_076546402.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690287.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_076690287.1:740..8908"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_076546403            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546403
VERSION     XP_076546403.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690288.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_076690288.1:573..8741"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_076546404            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546404
VERSION     XP_076546404.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690289.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_076690289.1:624..8792"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_034188229            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188229
VERSION     XP_034188229.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188229.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_034332338.2:250..8418"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_076546405            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546405
VERSION     XP_076546405.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690290.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_076690290.1:460..8628"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_034188265            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188265
VERSION     XP_034188265.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332374.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188265.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_034332374.2:191..8359"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_034188256            2722 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188256
VERSION     XP_034188256.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332365.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188256.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="molecule interacting with CasL isoform X1"
                     /calculated_mol_wt=308452
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2652
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2652
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2722
                     /gene="Mical"
                     /coded_by="XM_034332365.2:241..8409"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv
     2701 fftnlielfv nfyrehltlf ks
//
LOCUS       XP_076546406            2715 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546406
VERSION     XP_076546406.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690291.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2715
                     /product="molecule interacting with CasL isoform X2"
                     /calculated_mol_wt=307647
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          975..1031
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(975,978,996,999,1002,1005,1027,1030)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1177..1369
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <1998..2645
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2523..2645
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2715
                     /gene="Mical"
                     /coded_by="XM_076690291.1:1237..9384"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq flarqikmek
      901 kgrdqkesds kyadidstlk nidrkikegn vlghnkvsam aeqfsnksqd aepkvqksnt
      961 kptitlpaqg gsemchfcnk rvylmerlsa egkffhrgcf rceycstslr ignhtfdrek
     1021 nggrfyctqh fgfsgtlkar aekkriasvn kenipnspvn iktpekakap legvvgldll
     1081 drgqtperie fenlaemsda eepqsqided vwtdrnfgas taemgssddi sdmsdsdddn
     1141 evyeeaidqp lttegtlela knwtlryshp hatlgqsdtg sneyedssde ytneddestt
     1201 atededdira relrkqevwl knptrssdtd sgsetevasn egtsdeseen sateistdse
     1261 fehdgatptl hkipeitind syirktrgny vepkkvqvks kvissvngnl kqdkdpdekl
     1321 qmkadhskhp pragkestln eqesnntnrv plmnprkgdy llnrthsteg iasklslelk
     1381 kkyllggsaf ggsvmksgsa snvdtqlrnl tdaisqhqkl lnpapepspt mqaflqgtsk
     1441 lrssnaqlsp isptaifsss pvksysanrs sqnllnndnp mykatilpem sktqlpdlvk
     1501 essilkskta pnivcgesks taskeltkeq vsssqtatmv nstqtmenvk nsqnvdtnes
     1561 ngfrprsplh etsiivpqvd wnkrkaeake tvssedseid sdslsstdge veegaqnqql
     1621 savnlspprl qihstdgdll ldeqadrykd fddaqsfepd siecpflrde etndniitsa
     1681 dpvssmnkts meleivknei ehlelqddnk ksisncstpt svastvsnkq ddseendvtt
     1741 aaftetefse wardgevlvs ddlrdvefni npefittrrn gtllgktrns sitiakeedl
     1801 tdmeldpmky nrkleipvnd tskllangen idfmdtdnds llddslqdas nmvilknrgy
     1861 vefvniksap tssssqerli tdapiaqlda endscseeea yndrnviein pvtmddvmnk
     1921 lngtvtkten eivekadake nsvqhdqppv eavnkelfqs meedsllive paedtttsev
     1981 itilaspvnp qvsivptqiq easqeeepkt aadssnpdyl eyvkrlqsri aefsnakdsi
     2041 dvrkskrkns kssiqtrtae miaeeiksqd aiinnsfnsp atsrkleeit rerskqknvi
     2101 qdllmdkvea hkqksaekka rraarassft stpvlspirp pvsntslvnk ftptsivtpe
     2161 nsplrttfad akkcleteet qrreeeineg skkneiqsse naslteanne aennfktpva
     2221 pprlkheevr rtaekarqda rerarlksde dlglspedki kelrmkvarr qlsmeerksk
     2281 eetqpeprmr lyssgenktg lklqtskstd nmkavveavn fsglstantk smdellrtsg
     2341 spksntsitv vkrdkkqktk dperrksiiq avsdfffkke sspspsnqkd klsmfrlssk
     2401 skgklyksys egsdldkivp pktntrpksv cegmltrnfl nenappippp plnytiptth
     2461 vsddslsedd tkttavstsc mhkatvtegs cnsisrktkt tkriarqaql krlrmaqeiq
     2521 rkleetevkq relesrgvsv ekalrgegec sdreeadllr ewfdlmkert elrryekell
     2581 vraqevqled rherlqqelr erladddnkk tsddvkkege iltemleiva krdslialle
     2641 eerqrwckst cqtltsfpky tvacsshvpn ffthnfnnss fvftltvili ayvfftnlie
     2701 lfvnfyrehl tlfks
//
LOCUS       XP_076546407            2714 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546407
VERSION     XP_076546407.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690292.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2714
                     /product="molecule interacting with CasL isoform X3"
                     /calculated_mol_wt=307542
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1176..1368
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <1997..2644
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2522..2644
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2714
                     /gene="Mical"
                     /coded_by="XM_076690292.1:1238..9382"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspakapl egvvgldlld
     1081 rgqtperief enlaemsdae epqsqidedv wtdrnfgast aemgssddis dmsdsdddne
     1141 vyeeaidqpl ttegtlelak nwtlryshph atlgqsdtgs neyedssdey tneddestta
     1201 tededdirar elrkqevwlk nptrssdtds gsetevasne gtsdeseens ateistdsef
     1261 ehdgatptlh kipeitinds yirktrgnyv epkkvqvksk vissvngnlk qdkdpdeklq
     1321 mkadhskhpp ragkestlne qesnntnrvp lmnprkgdyl lnrthstegi asklslelkk
     1381 kyllggsafg gsvmksgsas nvdtqlrnlt daisqhqkll npapepsptm qaflqgtskl
     1441 rssnaqlspi sptaifsssp vksysanrss qnllnndnpm ykatilpems ktqlpdlvke
     1501 ssilksktap nivcgeskst askeltkeqv sssqtatmvn stqtmenvkn sqnvdtnesn
     1561 gfrprsplhe tsiivpqvdw nkrkaeaket vssedseids dslsstdgev eegaqnqqls
     1621 avnlspprlq ihstdgdlll deqadrykdf ddaqsfepds iecpflrdee tndniitsad
     1681 pvssmnktsm eleivkneie hlelqddnkk sisncstpts vastvsnkqd dseendvtta
     1741 aftetefsew ardgevlvsd dlrdvefnin pefittrrng tllgktrnss itiakeedlt
     1801 dmeldpmkyn rkleipvndt skllangeni dfmdtdndsl lddslqdasn mvilknrgyv
     1861 efvniksapt ssssqerlit dapiaqldae ndscseeeay ndrnvieinp vtmddvmnkl
     1921 ngtvtktene ivekadaken svqhdqppve avnkelfqsm eedsllivep aedtttsevi
     1981 tilaspvnpq vsivptqiqe asqeeepkta adssnpdyle yvkrlqsria efsnakdsid
     2041 vrkskrknsk ssiqtrtaem iaeeiksqda iinnsfnspa tsrkleeitr erskqknviq
     2101 dllmdkveah kqksaekkar raarassfts tpvlspirpp vsntslvnkf tptsivtpen
     2161 splrttfada kkcleteetq rreeeinegs kkneiqssen aslteannea ennfktpvap
     2221 prlkheevrr taekarqdar erarlksded lglspedkik elrmkvarrq lsmeerkske
     2281 etqpeprmrl yssgenktgl klqtskstdn mkavveavnf sglstantks mdellrtsgs
     2341 pksntsitvv krdkkqktkd perrksiiqa vsdfffkkes spspsnqkdk lsmfrlssks
     2401 kgklyksyse gsdldkivpp ktntrpksvc egmltrnfln enappipppp lnytiptthv
     2461 sddslseddt kttavstscm hkatvtegsc nsisrktktt kriarqaqlk rlrmaqeiqr
     2521 kleetevkqr elesrgvsve kalrgegecs dreeadllre wfdlmkerte lrryekellv
     2581 raqevqledr herlqqelre rladddnkkt sddvkkegei ltemleivak rdsliallee
     2641 erqrwckstc qtltsfpkyt vacsshvpnf fthnfnnssf vftltvilia yvfftnliel
     2701 fvnfyrehlt lfks
//
LOCUS       XP_076546408            2712 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546408
VERSION     XP_076546408.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690293.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2712
                     /product="molecule interacting with CasL isoform X4"
                     /calculated_mol_wt=307322
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2642
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2520..2642
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2712
                     /gene="Mical"
                     /coded_by="XM_076690293.1:1237..9375"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg kldkivppkt ntrpksvceg mltrnflnen appippppln ytiptthvsd
     2461 dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl rmaqeiqrkl
     2521 eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr ryekellvra
     2581 qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd slialleeer
     2641 qrwckstcqt ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv fftnlielfv
     2701 nfyrehltlf ks
//
LOCUS       XP_076546409            2683 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546409
VERSION     XP_076546409.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690294.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2683
                     /product="molecule interacting with CasL isoform X5"
                     /calculated_mol_wt=303792
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          982..1038
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(982,985,1003,1006,1009,1012,1034,1037)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1184..1376
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <2005..2661
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2530..2660
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2683
                     /gene="Mical"
                     /coded_by="XM_076690294.1:1237..9288"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla
      901 rqikmekkgr dqkesdskya didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep
      961 kvqksntkpt itlpaqggse mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign
     1021 htfdrekngg rfyctqhfgf sgtlkaraek kriasvnken ipnspvnikt pekakapleg
     1081 vvgldlldrg qtperiefen laemsdaeep qsqidedvwt drnfgastae mgssddisdm
     1141 sdsdddnevy eeaidqpltt egtlelaknw tlryshphat lgqsdtgsne yedssdeytn
     1201 eddesttate deddirarel rkqevwlknp trssdtdsgs etevasnegt sdeseensat
     1261 eistdsefeh dgatptlhki peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd
     1321 kdpdeklqmk adhskhppra gkestlneqe snntnrvplm nprkgdylln rthstegias
     1381 klslelkkky llggsafggs vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa
     1441 flqgtsklrs snaqlspisp taifssspvk sysanrssqn llnndnpmyk atilpemskt
     1501 qlpdlvkess ilksktapni vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq
     1561 nvdtnesngf rprsplhets iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee
     1621 gaqnqqlsav nlspprlqih stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn
     1681 dniitsadpv ssmnktsmel eivkneiehl elqddnkksi sncstptsva stvsnkqdds
     1741 eendvttaaf tetefsewar dgevlvsddl rdvefninpe fittrrngtl lgktrnssit
     1801 iakeedltdm eldpmkynrk leipvndtsk llangenidf mdtdndslld dslqdasnmv
     1861 ilknrgyvef vniksaptss ssqerlitda piaqldaend scseeeaynd rnvieinpvt
     1921 mddvmnklng tvtkteneiv ekadakensv qhdqppveav nkelfqsmee dsllivepae
     1981 dtttseviti laspvnpqvs ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef
     2041 snakdsidvr kskrknskss iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer
     2101 skqknviqdl lmdkveahkq ksaekkarra arassftstp vlspirppvs ntslvnkftp
     2161 tsivtpensp lrttfadakk cleteetqrr eeeinegskk neiqssenas lteanneaen
     2221 nfktpvappr lkheevrrta ekarqdarer arlksdedlg lspedkikel rmkvarrqls
     2281 meerkskeet qpeprmrlys sgenktglkl qtskstdnmk avveavnfsg lstantksmd
     2341 ellrtsgspk sntsitvvkr dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls
     2401 mfrlsskskg klyksysegs dldkivppkt ntrpksvceg mltrnflnen appippppln
     2461 ytiptthvsd dslseddtkt tavstscmhk atvtegscns isrktkttkr iarqaqlkrl
     2521 rmaqeiqrkl eetevkqrel esrgvsveka lrgegecsdr eeadllrewf dlmkertelr
     2581 ryekellvra qevqledrhe rlqqelrerl adddnkktsd dvkkegeilt emleivakrd
     2641 slialleeer qryqdedrdl eaqmlakglr ltpikkcgpk ysv
//
LOCUS       XP_076546410            2642 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546410
VERSION     XP_076546410.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690295.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2642
                     /product="molecule interacting with CasL isoform X6"
                     /calculated_mol_wt=298380
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          902..958
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(902,905,923,926,929,932,954,957)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1104..1296
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <1925..2572
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2450..2572
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2642
                     /gene="Mical"
                     /coded_by="XM_076690295.1:1237..9165"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tvdrepkiha ghrrnsidee fsgriksied klkgstpsek kprdllraig kiektdwnvk
      781 eiekkieenk mgrsvrhekm ervpkwsreq isicfiqfla rqikmekkgr dqkesdskya
      841 didstlknid rkikegnvlg hnkvsamaeq fsnksqdaep kvqksntkpt itlpaqggse
      901 mchfcnkrvy lmerlsaegk ffhrgcfrce ycstslrign htfdrekngg rfyctqhfgf
      961 sgtlkaraek kriasvnken ipnspvnikt pekakapleg vvgldlldrg qtperiefen
     1021 laemsdaeep qsqidedvwt drnfgastae mgssddisdm sdsdddnevy eeaidqpltt
     1081 egtlelaknw tlryshphat lgqsdtgsne yedssdeytn eddesttate deddirarel
     1141 rkqevwlknp trssdtdsgs etevasnegt sdeseensat eistdsefeh dgatptlhki
     1201 peitindsyi rktrgnyvep kkvqvkskvi ssvngnlkqd kdpdeklqmk adhskhppra
     1261 gkestlneqe snntnrvplm nprkgdylln rthstegias klslelkkky llggsafggs
     1321 vmksgsasnv dtqlrnltda isqhqkllnp apepsptmqa flqgtsklrs snaqlspisp
     1381 taifssspvk sysanrssqn llnndnpmyk atilpemskt qlpdlvkess ilksktapni
     1441 vcgeskstas keltkeqvss sqtatmvnst qtmenvknsq nvdtnesngf rprsplhets
     1501 iivpqvdwnk rkaeaketvs sedseidsds lsstdgevee gaqnqqlsav nlspprlqih
     1561 stdgdlllde qadrykdfdd aqsfepdsie cpflrdeetn dniitsadpv ssmnktsmel
     1621 eivkneiehl elqddnkksi sncstptsva stvsnkqdds eendvttaaf tetefsewar
     1681 dgevlvsddl rdvefninpe fittrrngtl lgktrnssit iakeedltdm eldpmkynrk
     1741 leipvndtsk llangenidf mdtdndslld dslqdasnmv ilknrgyvef vniksaptss
     1801 ssqerlitda piaqldaend scseeeaynd rnvieinpvt mddvmnklng tvtkteneiv
     1861 ekadakensv qhdqppveav nkelfqsmee dsllivepae dtttseviti laspvnpqvs
     1921 ivptqiqeas qeeepktaad ssnpdyleyv krlqsriaef snakdsidvr kskrknskss
     1981 iqtrtaemia eeiksqdaii nnsfnspats rkleeitrer skqknviqdl lmdkveahkq
     2041 ksaekkarra arassftstp vlspirppvs ntslvnkftp tsivtpensp lrttfadakk
     2101 cleteetqrr eeeinegskk neiqssenas lteanneaen nfktpvappr lkheevrrta
     2161 ekarqdarer arlksdedlg lspedkikel rmkvarrqls meerkskeet qpeprmrlys
     2221 sgenktglkl qtskstdnmk avveavnfsg lstantksmd ellrtsgspk sntsitvvkr
     2281 dkkqktkdpe rrksiiqavs dfffkkessp spsnqkdkls mfrlsskskg klyksysegs
     2341 dldkivppkt ntrpksvceg mltrnflnen appippppln ytiptthvsd dslseddtkt
     2401 tavstscmhk atvtegscns isrktkttkr iarqaqlkrl rmaqeiqrkl eetevkqrel
     2461 esrgvsveka lrgegecsdr eeadllrewf dlmkertelr ryekellvra qevqledrhe
     2521 rlqqelrerl adddnkktsd dvkkegeilt emleivakrd slialleeer qrwckstcqt
     2581 ltsfpkytva csshvpnfft hnfnnssfvf tltviliayv fftnlielfv nfyrehltlf
     2641 ks
//
LOCUS       XP_076546411            2705 aa            linear   INV 03-SEP-2025
DEFINITION  molecule interacting with CasL isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546411
VERSION     XP_076546411.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690296.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2705
                     /product="molecule interacting with CasL isoform X7"
                     /calculated_mol_wt=306517
     Region          93..>234
                     /region_name="UbiH"
                     /note="2-polyprenyl-6-methoxyphenol hydroxylase and
                     related FAD-dependent oxidoreductases [Coenzyme transport
                     and metabolism, Energy production and conversion];
                     COG0654"
                     /db_xref="CDD:440419"
     Region          531..635
                     /region_name="CH_MICAL_EHBP-like"
                     /note="calponin homology (CH) domain found in the MICAL
                     and EHBP families; cd22198"
                     /db_xref="CDD:409188"
     Site            order(531,535,585,587..588,591..592,594,604..611,619,
                     621..622,624..625,628..629,632)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409188"
     Region          975..1031
                     /region_name="LIM_Mical"
                     /note="The LIM domain of Mical (molecule interacting with
                     CasL); cd09439"
                     /db_xref="CDD:188823"
     Site            order(975,978,996,999,1002,1005,1027,1030)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188823"
     Region          <1177..1369
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          <1998..2635
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          2513..2635
                     /region_name="bMERB_dom"
                     /note="Bivalent Mical/EHBP Rab binding domain; pfam12130"
                     /db_xref="CDD:463467"
     CDS             1..2705
                     /gene="Mical"
                     /coded_by="XM_076690296.1:1237..9354"
                     /db_xref="GeneID:117607963"
ORIGIN      
        1 mdhqqpgrkq tmnlsemaia ndmfdqfcna ttlksilghf rdlceilkir pntiaqfypk
       61 lkaklrtwka qalwkkfdqr anhkcynrgk acpntrvlii gggpcglrsa ieaqllgakv
      121 vvvekrdrms rnnvlhlwpf viqdlrglga kkffgkfcag sidhisirql qcillkvali
      181 lgvefhesvs fdslipppen qeegkigwra kttpsdhpvs qyefdvliga dgkrntlegf
      241 krkefrgkla iaitanfink rteaearvee isgvafifnq kffkelylet gidlenivyy
      301 kddthyfvmt akkhslidkg vilqdhadta kllakenvdr ealmhyarea aefsteyqmm
      361 dmefavnhyg qpdvamfdft smyaaenasr vlerrghrll milvgdslle pfwptgsgca
      421 rgflssldac waikgwgasv spleviaere siyrllgqtt penlnrdyaa ytldphtryp
      481 nlnvssvtpi qvrslldtdd pdsikqppvq sdidipkkrr rrdlrgdtqv hpdtllrwlq
      541 kqvalydsiq iedmgasfks glaicaiihr ycpdlidfhs lnpndpitnn qlafdileke
      601 lgippimtge emaqcdvpdk lamfsyltqi yevfrgeipy ikhpklepen derpthskql
      661 sqltpdqkvq lidrivrqds gtrtrhsrsr hnenmnpadk ksdtisrrsr krrstekmga
      721 tveerqkrla dieknraerm rrrqylryma tqqfyksmqm lqanakrekd epfedysifl
      781 yrqtapdfkd rvkyleqkil ypdrepkiha ghrrnsidee fsgriksied klkgstpsek
      841 kprdllraig kiektdwnvk eiekkieenk mgrsvrhekm ervpkwsreq flarqikmek
      901 kgrdqkesds kyadidstlk nidrkikegn vlghnkvsam aeqfsnksqd aepkvqksnt
      961 kptitlpaqg gsemchfcnk rvylmerlsa egkffhrgcf rceycstslr ignhtfdrek
     1021 nggrfyctqh fgfsgtlkar aekkriasvn kenipnspvn iktpekakap legvvgldll
     1081 drgqtperie fenlaemsda eepqsqided vwtdrnfgas taemgssddi sdmsdsdddn
     1141 evyeeaidqp lttegtlela knwtlryshp hatlgqsdtg sneyedssde ytneddestt
     1201 atededdira relrkqevwl knptrssdtd sgsetevasn egtsdeseen sateistdse
     1261 fehdgatptl hkipeitind syirktrgny vepkkvqvks kvissvngnl kqdkdpdekl
     1321 qmkadhskhp pragkestln eqesnntnrv plmnprkgdy llnrthsteg iasklslelk
     1381 kkyllggsaf ggsvmksgsa snvdtqlrnl tdaisqhqkl lnpapepspt mqaflqgtsk
     1441 lrssnaqlsp isptaifsss pvksysanrs sqnllnndnp mykatilpem sktqlpdlvk
     1501 essilkskta pnivcgesks taskeltkeq vsssqtatmv nstqtmenvk nsqnvdtnes
     1561 ngfrprsplh etsiivpqvd wnkrkaeake tvssedseid sdslsstdge veegaqnqql
     1621 savnlspprl qihstdgdll ldeqadrykd fddaqsfepd siecpflrde etndniitsa
     1681 dpvssmnkts meleivknei ehlelqddnk ksisncstpt svastvsnkq ddseendvtt
     1741 aaftetefse wardgevlvs ddlrdvefni npefittrrn gtllgktrns sitiakeedl
     1801 tdmeldpmky nrkleipvnd tskllangen idfmdtdnds llddslqdas nmvilknrgy
     1861 vefvniksap tssssqerli tdapiaqlda endscseeea yndrnviein pvtmddvmnk
     1921 lngtvtkten eivekadake nsvqhdqppv eavnkelfqs meedsllive paedtttsev
     1981 itilaspvnp qvsivptqiq easqeeepkt aadssnpdyl eyvkrlqsri aefsnakdsi
     2041 dvrkskrkns kssiqtrtae miaeeiksqd aiinnsfnsp atsrkleeit rerskqknvi
     2101 qdllmdkvea hkqksaekka rraarassft stpvlspirp pvsntslvnk ftptsivtpe
     2161 nsplrttfad akkcleteet qrreeeineg skkneiqsse naslteanne aennfktpva
     2221 pprlkheevr rtaekarqda rerarlksde dlglspedki kelrmkvarr qlsmeerksk
     2281 eetqpeprmr lyssgenktg lklqtskstd nmkavveavn fsglstantk smdellrtsg
     2341 spksntsitv vkrdkkqktk dperrksiiq avsdfffkke sspspsnqkd klsmfrlssk
     2401 skgkldkivp pktntrpksv cegmltrnfl nenappippp plnytiptth vsddslsedd
     2461 tkttavstsc mhkatvtegs cnsisrktkt tkriarqaql krlrmaqeiq rkleetevkq
     2521 relesrgvsv ekalrgegec sdreeadllr ewfdlmkert elrryekell vraqevqled
     2581 rherlqqelr erladddnkk tsddvkkege iltemleiva krdslialle eerqrwckst
     2641 cqtltsfpky tvacsshvpn ffthnfnnss fvftltvili ayvfftnlie lfvnfyrehl
     2701 tlfks
//
LOCUS       XP_034189082            1743 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608298 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189082
VERSION     XP_034189082.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189082.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1743
                     /product="uncharacterized protein LOC117608298 isoform X1"
                     /calculated_mol_wt=195487
     Region          <837..1602
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1743
                     /gene="LOC117608298"
                     /coded_by="XM_034333191.2:213..5444"
                     /db_xref="GeneID:117608298"
ORIGIN      
        1 mprstrdsee evdpkpgrkt rltdvvsvds plrrssriks vkqnesspes lssdtsnvsn
       61 tqtkvtrrrt gimdnittte kqrslrsrmn sgsldvsevt engtntmtpt krdrnsitgd
      121 vlnktnnrrs tqftragses ktspirvtrn trstsmepes vavnkhmdkn esecvhtssr
      181 mrrrtlivps eatvveekee nmkvlvvtld rtlpnvsevk emdlndplks aqkhdpmssa
      241 ednlnsscqs lneksienss vkelsnniae klkeeesand sstlknkptk ksesneqkse
      301 siqetcnkdq gsepveetpt eleevpkkee lkeeesands stlkskptek sgsdeqnses
      361 iqetcnknqr sevvegndtd ltapnslqdi patewkeknv andsvleirn tedmkiqkkd
      421 afdytsklav evhslsepil nvssdknvpl kissdlssce sfelvqcmdd vmttddeklt
      481 etsltlnket tkvhneesne ivndstisnv ekainantes gkdniksnts mndqdgvkee
      541 vhkpdtlgnv qndygmsctn tcsspnltnt vpltspdkss tasaecspil knketselks
      601 inktntsfpn tnsgkssnad qdsvtdvvks sppvtvkepa vtptahdekv pkkglkeees
      661 tndlstlkkk lteksgsdeq ksesiqetcn knqeseaves ndsdiiatnl fqdipdvewk
      721 ennsgkstnt dgdsvtdvvk ssapftvtes pteldeevtk keelkekefa ndsstlknkp
      781 teksgsdeqk sesiqetcnk nkgseavesn dsdttatdlf qdipaaewne kntdvdknsi
      841 hlvsteqfen emetecdlvl idkeawlaae nmkaarekea sdydsddtvv lkirldsvia
      901 qknektkmnr svdkmndskd aisnendidn ettntkdfkd eienenqdts iteqrtehsa
      961 ssrkllerks lnkshkitea eenlsesitk kqkspnksts kadvsrksln ksiqkemene
     1021 stsarkskeq rslnisskeq rscteesdte akdssisvkk sngkkkfsrk rstirtiesn
     1081 sddnksesgd santelpkfm lheasntgsn esdksmdsdi ekeynlggkd tskfsdddvr
     1141 edecrasete ssdpedngsd ledfivddve edeeseegdt aneeseegek anekikieel
     1201 htdentqenq ekeikeqnea mqvqepdvie dkikegmtee elqneneqen iedvdklidt
     1261 sskrksklei sdrlstsqin rkkklkkvdt sqsekskken slsdlllkqk vlsvadnile
     1321 vdsqkkykkr kqtaekmntt iliedsfled vdsqivskdk knvvstidad knkkvekvfl
     1381 nekkkkitht dinkdveeev lheeitntkt kitkkpkkik kkqekeiten vldicsgnqv
     1441 sgkrkkkise pllneeitst rtskktpkls kdivlqddet aveqmpkkkk kgldkklakk
     1501 qnvsssdtsq nknisaekls kkqhklvakd ansevksvkk snklqqsvsd sneeltivkf
     1561 seareeaskt vkritdgikv skekkkkkqk ermqevelen ekpkskkqki ndnktastkg
     1621 ikrlsdefle nlsdvpskpl kkkklskikn qispsrsmfd skankvenvy veedfdtvss
     1681 yggttqfave niqkmkkkkk ipgvvsfkqk miarnsrqpv saylmylekq kvagkdtfcn
     1741 kpy
//
LOCUS       XP_034189089            1687 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608298 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189089
VERSION     XP_034189089.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189089.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1687
                     /product="uncharacterized protein LOC117608298 isoform X2"
                     /calculated_mol_wt=189267
     Region          <781..1546
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1687
                     /gene="LOC117608298"
                     /coded_by="XM_034333198.2:219..5282"
                     /db_xref="GeneID:117608298"
ORIGIN      
        1 mprstrdsee evdpkpgrkt rltdvvsvds plrrssriks vkqnesspes lssdtsnvsn
       61 tqtkvtrrrt gimdnittte kqrslrsrmn sgsldvsevt engtntmtpt krdrnsitgd
      121 vlnktnnrrs tqftragses ktspirvtrn trstsmepes vavnkhmdkn esecvhtssr
      181 mrrrtlivps eatvveekee nmkvlvvtld rtlpnvsevk emdlndplks aqkhdpmssa
      241 ednlnsscqs lneksienss etpteleevp kkeelkeees andsstlksk pteksgsdeq
      301 nsesiqetcn knqrsevveg ndtdltapns lqdipatewk eknvandsvl eirntedmki
      361 qkkdafdyts klavevhsls epilnvssdk nvplkissdl sscesfelvq cmddvmttdd
      421 ekltetsltl nkettkvhne esneivndst isnvekaina ntesgkdnik sntsmndqdg
      481 vkeevhkpdt lgnvqndygm sctntcsspn ltntvpltsp dksstasaec spilknkets
      541 elksinktnt sfpntnsgks snadqdsvtd vvkssppvtv kepavtptah dekvpkkglk
      601 eeestndlst lkkklteksg sdeqksesiq etcnknqese avesndsdii atnlfqdipd
      661 vewkennsgk stntdgdsvt dvvkssapft vtesptelde evtkkeelke kefandsstl
      721 knkpteksgs deqksesiqe tcnknkgsea vesndsdtta tdlfqdipaa ewnekntdvd
      781 knsihlvste qfenemetec dlvlidkeaw laaenmkaar ekeasdydsd dtvvlkirld
      841 sviaqknekt kmnrsvdkmn dskdaisnen didnettntk dfkdeienen qdtsiteqrt
      901 ehsassrkll erkslnkshk iteaeenlse sitkkqkspn kstskadvsr kslnksiqke
      961 menestsark skeqrslnis skeqrsctee sdteakdssi svkksngkkk fsrkrstirt
     1021 iesnsddnks esgdsantel pkfmlheasn tgsnesdksm dsdiekeynl ggkdtskfsd
     1081 ddvredecra setessdped ngsdledfiv ddveedeese egdtaneese egekanekik
     1141 ieelhtdent qenqekeike qneamqvqep dviedkikeg mteeelqnen eqeniedvdk
     1201 lidtsskrks kleisdrlst sqinrkkklk kvdtsqseks kkenslsdll lkqkvlsvad
     1261 nilevdsqkk ykkrkqtaek mnttilieds fledvdsqiv skdkknvvst idadknkkve
     1321 kvflnekkkk ithtdinkdv eeevlheeit ntktkitkkp kkikkkqeke itenvldics
     1381 gnqvsgkrkk kisepllnee itstrtskkt pklskdivlq ddetaveqmp kkkkkgldkk
     1441 lakkqnvsss dtsqnknisa eklskkqhkl vakdansevk svkksnklqq svsdsneelt
     1501 ivkfsearee asktvkritd gikvskekkk kkqkermqev elenekpksk kqkindnkta
     1561 stkgikrlsd eflenlsdvp skplkkkkls kiknqispsr smfdskankv envyveedfd
     1621 tvssyggttq faveniqkmk kkkkipgvvs fkqkmiarns rqpvsaylmy lekqkvagkd
     1681 tfcnkpy
//
LOCUS       XP_034189100            1655 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608298 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189100
VERSION     XP_034189100.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333209.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189100.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1655
                     /product="uncharacterized protein LOC117608298 isoform X3"
                     /calculated_mol_wt=185528
     Region          <749..1514
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1655
                     /gene="LOC117608298"
                     /coded_by="XM_034333209.2:263..5230"
                     /db_xref="GeneID:117608298"
ORIGIN      
        1 mnsgsldvse vtengtntmt ptkrdrnsit gdvlnktnnr rstqftrags esktspirvt
       61 rntrstsmep esvavnkhmd knesecvhts srmrrrtliv pseatvveek eenmkvlvvt
      121 ldrtlpnvse vkemdlndpl ksaqkhdpms saednlnssc qslneksien ssvkelsnni
      181 aeklkeeesa ndsstlknkp tkksesneqk sesiqetcnk dqgsepveet pteleevpkk
      241 eelkeeesan dsstlkskpt eksgsdeqns esiqetcnkn qrsevvegnd tdltapnslq
      301 dipatewkek nvandsvlei rntedmkiqk kdafdytskl avevhslsep ilnvssdknv
      361 plkissdlss cesfelvqcm ddvmttddek ltetsltlnk ettkvhnees neivndstis
      421 nvekainant esgkdniksn tsmndqdgvk eevhkpdtlg nvqndygmsc tntcsspnlt
      481 ntvpltspdk sstasaecsp ilknketsel ksinktntsf pntnsgkssn adqdsvtdvv
      541 kssppvtvke pavtptahde kvpkkglkee estndlstlk kklteksgsd eqksesiqet
      601 cnknqeseav esndsdiiat nlfqdipdve wkennsgkst ntdgdsvtdv vkssapftvt
      661 espteldeev tkkeelkeke fandsstlkn kpteksgsde qksesiqetc nknkgseave
      721 sndsdttatd lfqdipaaew nekntdvdkn sihlvsteqf enemetecdl vlidkeawla
      781 aenmkaarek easdydsddt vvlkirldsv iaqknektkm nrsvdkmnds kdaisnendi
      841 dnettntkdf kdeienenqd tsiteqrteh sassrkller kslnkshkit eaeenlsesi
      901 tkkqkspnks tskadvsrks lnksiqkeme nestsarksk eqrslnissk eqrscteesd
      961 teakdssisv kksngkkkfs rkrstirtie snsddnkses gdsantelpk fmlheasntg
     1021 snesdksmds diekeynlgg kdtskfsddd vredecrase tessdpedng sdledfivdd
     1081 veedeeseeg dtaneeseeg ekanekikie elhtdentqe nqekeikeqn eamqvqepdv
     1141 iedkikegmt eeelqneneq eniedvdkli dtsskrkskl eisdrlstsq inrkkklkkv
     1201 dtsqsekskk enslsdlllk qkvlsvadni levdsqkkyk krkqtaekmn ttiliedsfl
     1261 edvdsqivsk dkknvvstid adknkkvekv flnekkkkit htdinkdvee evlheeitnt
     1321 ktkitkkpkk ikkkqekeit envldicsgn qvsgkrkkki sepllneeit strtskktpk
     1381 lskdivlqdd etaveqmpkk kkkgldkkla kkqnvsssdt sqnknisaek lskkqhklva
     1441 kdansevksv kksnklqqsv sdsneeltiv kfseareeas ktvkritdgi kvskekkkkk
     1501 qkermqevel enekpkskkq kindnktast kgikrlsdef lenlsdvpsk plkkkklski
     1561 knqispsrsm fdskankven vyveedfdtv ssyggttqfa veniqkmkkk kkipgvvsfk
     1621 qkmiarnsrq pvsaylmyle kqkvagkdtf cnkpy
//
LOCUS       XP_076546412            1068 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608310 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546412
VERSION     XP_076546412.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690297.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1068
                     /product="uncharacterized protein LOC117608310 isoform X1"
                     /calculated_mol_wt=120081
     CDS             1..1068
                     /gene="LOC117608310"
                     /coded_by="XM_076690297.1:61..3267"
                     /db_xref="GeneID:117608310"
ORIGIN      
        1 msntednsdy scqavnekst enirvkklpn nlaekspskf sedvdkdtst ndnnimsdnk
       61 nkseetgslp ahvvtkmepl vhnisqsfsq kagqdtskel glkienllnn smekhedfsn
      121 kenlaanvir depniknahs ntlvqsskss svrnsnklvk ekcksscnls srsrdsrste
      181 lmddtivnvd gdvandsmfe ilnsdntskl avetdslses ilnvssdknv plkissdlss
      241 cesielvqcm ddvmtmddek ltetritlnk eptevhnees neivndstis nvekaisane
      301 esgkdnikln tsmndeddvk eevhkpdtlr nvqndygmsc tntysspnli ntvpltspdk
      361 sstanpeysp ilknketsgl tsiiktntsf lntksakstn anqdsvtdvv kssppstvte
      421 tpsklaekvs kkeesannss tlknkpreks eshkridsai ereynlggkd tskfsdddvr
      481 gdecrasetd ssdledngsd lqdlivndve edeeseeege kanekieiee lhtdentqen
      541 qkreikeqde amqvqepdvi qdkikegmte eesqneneqe nienvdklid tsskrkskle
      601 isdrlstsqi nrkkklkkvd tsqsekskke nslsdlllkq kvlsvadnil evdsqkkhkk
      661 rkqtaekmnt tiliedsfle dvdsqivskd kknvvstida dknkkveevf lnekkkkith
      721 tdinkdveee vlheeitntk tkitkkpkki kkkqekeite nltdicsrhq vsgkkkkkis
      781 eallneeitt triskkapkl skniavryve taveklpkkk krlvskdass qekalakklp
      841 kkqnlssndt sqdknittek lskkriklve kdtksevkpv kksnklqqsa divsdsnegp
      901 tvvkfseaqe ealkavksit dsikdskere kkkqkgrmqe velenekpks kkqkikdnkt
      961 egtkgikrrt deflvnlsdv pskplkkrkf lkikeqilps cspfnlntne veniyvekqf
     1021 dtvsscgstt qfavvniqki kkkkkipgia sfkkkmiarn srqtilyi
//
LOCUS       XP_034189113            1068 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608310 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189113
VERSION     XP_034189113.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333222.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189113.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1068
                     /product="uncharacterized protein LOC117608310 isoform X1"
                     /calculated_mol_wt=120081
     CDS             1..1068
                     /gene="LOC117608310"
                     /coded_by="XM_034333222.2:62..3268"
                     /db_xref="GeneID:117608310"
ORIGIN      
        1 msntednsdy scqavnekst enirvkklpn nlaekspskf sedvdkdtst ndnnimsdnk
       61 nkseetgslp ahvvtkmepl vhnisqsfsq kagqdtskel glkienllnn smekhedfsn
      121 kenlaanvir depniknahs ntlvqsskss svrnsnklvk ekcksscnls srsrdsrste
      181 lmddtivnvd gdvandsmfe ilnsdntskl avetdslses ilnvssdknv plkissdlss
      241 cesielvqcm ddvmtmddek ltetritlnk eptevhnees neivndstis nvekaisane
      301 esgkdnikln tsmndeddvk eevhkpdtlr nvqndygmsc tntysspnli ntvpltspdk
      361 sstanpeysp ilknketsgl tsiiktntsf lntksakstn anqdsvtdvv kssppstvte
      421 tpsklaekvs kkeesannss tlknkpreks eshkridsai ereynlggkd tskfsdddvr
      481 gdecrasetd ssdledngsd lqdlivndve edeeseeege kanekieiee lhtdentqen
      541 qkreikeqde amqvqepdvi qdkikegmte eesqneneqe nienvdklid tsskrkskle
      601 isdrlstsqi nrkkklkkvd tsqsekskke nslsdlllkq kvlsvadnil evdsqkkhkk
      661 rkqtaekmnt tiliedsfle dvdsqivskd kknvvstida dknkkveevf lnekkkkith
      721 tdinkdveee vlheeitntk tkitkkpkki kkkqekeite nltdicsrhq vsgkkkkkis
      781 eallneeitt triskkapkl skniavryve taveklpkkk krlvskdass qekalakklp
      841 kkqnlssndt sqdknittek lskkriklve kdtksevkpv kksnklqqsa divsdsnegp
      901 tvvkfseaqe ealkavksit dsikdskere kkkqkgrmqe velenekpks kkqkikdnkt
      961 egtkgikrrt deflvnlsdv pskplkkrkf lkikeqilps cspfnlntne veniyvekqf
     1021 dtvsscgstt qfavvniqki kkkkkipgia sfkkkmiarn srqtilyi
//
LOCUS       XP_076546413            1059 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608310 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546413
VERSION     XP_076546413.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690298.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1059
                     /product="uncharacterized protein LOC117608310 isoform X2"
                     /calculated_mol_wt=119102
     Region          <414..933
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1059
                     /gene="LOC117608310"
                     /coded_by="XM_076690298.1:36..3215"
                     /db_xref="GeneID:117608310"
ORIGIN      
        1 msntednsdy scqavnekst enirekspsk fsedvdkdts tndnnimsdn knkseetgsl
       61 pahvvtkmep lvhnisqsfs qkagqdtske lglkienlln nsmekhedfs nkenlaanvi
      121 rdepniknah sntlvqssks ssvrnsnklv kekcksscnl ssrsrdsrst elmddtivnv
      181 dgdvandsmf eilnsdntsk lavetdslse silnvssdkn vplkissdls scesielvqc
      241 mddvmtmdde kltetritln keptevhnee sneivndsti snvekaisan eesgkdnikl
      301 ntsmndeddv keevhkpdtl rnvqndygms ctntysspnl intvpltspd ksstanpeys
      361 pilknketsg ltsiiktnts flntksakst nanqdsvtdv vkssppstvt etpsklaekv
      421 skkeesanns stlknkprek seshkridsa iereynlggk dtskfsdddv rgdecraset
      481 dssdledngs dlqdlivndv eedeeseeeg ekanekieie elhtdentqe nqkreikeqd
      541 eamqvqepdv iqdkikegmt eeesqneneq enienvdkli dtsskrkskl eisdrlstsq
      601 inrkkklkkv dtsqsekskk enslsdlllk qkvlsvadni levdsqkkhk krkqtaekmn
      661 ttiliedsfl edvdsqivsk dkknvvstid adknkkveev flnekkkkit htdinkdvee
      721 evlheeitnt ktkitkkpkk ikkkqekeit enltdicsrh qvsgkkkkki seallneeit
      781 ttriskkapk lskniavryv etaveklpkk kkrlvskdas sqekalakkl pkkqnlssnd
      841 tsqdknitte klskkriklv ekdtksevkp vkksnklqqs adivsdsneg ptvvkfseaq
      901 eealkavksi tdsikdsker ekkkqkgrmq evelenekpk skkqkikdnk tegtkgikrr
      961 tdeflvnlsd vpskplkkrk flkikeqilp scspfnlntn eveniyvekq fdtvsscgst
     1021 tqfavvniqk ikkkkkipgi asfkkkmiar nsrqtilyi
//
LOCUS       XP_034189126             684 aa            linear   INV 03-SEP-2025
DEFINITION  RNA exonuclease 5 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189126
VERSION     XP_034189126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="RNA exonuclease 5 isoform X1"
                     /calculated_mol_wt=77783
     Region          345..495
                     /region_name="REX1_like"
                     /note="DEDDh 3'-5' exonuclease domain of RNA exonuclease
                     1, -3 and similar eukaryotic proteins; cd06145"
                     /db_xref="CDD:99848"
     Site            order(348..351,353,427..428,430..432,481,486)
                     /site_type="active"
                     /db_xref="CDD:99848"
     Site            order(348..351,353,427..428,430..431,481,486)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:99848"
     Site            order(348,350,432,481,486)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99848"
     CDS             1..684
                     /gene="Rexo5"
                     /coded_by="XM_034333235.2:297..2351"
                     /db_xref="GeneID:117608317"
ORIGIN      
        1 mknltakqlq riekkkkkma alleitklnd kdreakilal ktasteidls ndidnneism
       61 eesynrkrpc dedlkgtcsg dstekedgei teeldplank kprlsgeeyl klkqelrerk
      121 krlksiprfh lkavgenasi sinvksedri piflsdiqhl llyslhghhs pylptrwchl
      181 ekynrvshtv alvieglsiy hfmayesmfp sitsnlehrv evvtpmiygg svvedlaavp
      241 itgvhcdkli eqygslqsal nstrdvikll ktvfpmyqte ptdikrilel pytdkfprtq
      301 lllslcqmve enypiplsge lakkygnyim tkdvyleata rspmigldce mckttmgele
      361 ltritlvdek mnviydslvk pdnpitdylt rfsgitkeml edvtttlsdi qqvlrkllpp
      421 dailvgqsln sdlhtlkmmh pyiidtsvif nitgdryrkt klqtlvrefl gekiqnstdg
      481 hcsaedsrac mklvqlklan svdygdavll grcdieilkm etakrspter slkteirkya
      541 tsifnhvtkd qktaavvgde evmneystfl tpsikimdde nfdkndqvrl vvadnnkqav
      601 trasqiameh aftlchirik keklgddrie ktfnivnkwv yklwqhmain glacvifage
      661 nnagngvcfl nikrevpenc vira
//
LOCUS       XP_034189136             684 aa            linear   INV 03-SEP-2025
DEFINITION  RNA exonuclease 5 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189136
VERSION     XP_034189136.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333245.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="RNA exonuclease 5 isoform X1"
                     /calculated_mol_wt=77783
     Region          345..495
                     /region_name="REX1_like"
                     /note="DEDDh 3'-5' exonuclease domain of RNA exonuclease
                     1, -3 and similar eukaryotic proteins; cd06145"
                     /db_xref="CDD:99848"
     Site            order(348..351,353,427..428,430..432,481,486)
                     /site_type="active"
                     /db_xref="CDD:99848"
     Site            order(348..351,353,427..428,430..431,481,486)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:99848"
     Site            order(348,350,432,481,486)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99848"
     CDS             1..684
                     /gene="Rexo5"
                     /coded_by="XM_034333245.2:174..2228"
                     /db_xref="GeneID:117608317"
ORIGIN      
        1 mknltakqlq riekkkkkma alleitklnd kdreakilal ktasteidls ndidnneism
       61 eesynrkrpc dedlkgtcsg dstekedgei teeldplank kprlsgeeyl klkqelrerk
      121 krlksiprfh lkavgenasi sinvksedri piflsdiqhl llyslhghhs pylptrwchl
      181 ekynrvshtv alvieglsiy hfmayesmfp sitsnlehrv evvtpmiygg svvedlaavp
      241 itgvhcdkli eqygslqsal nstrdvikll ktvfpmyqte ptdikrilel pytdkfprtq
      301 lllslcqmve enypiplsge lakkygnyim tkdvyleata rspmigldce mckttmgele
      361 ltritlvdek mnviydslvk pdnpitdylt rfsgitkeml edvtttlsdi qqvlrkllpp
      421 dailvgqsln sdlhtlkmmh pyiidtsvif nitgdryrkt klqtlvrefl gekiqnstdg
      481 hcsaedsrac mklvqlklan svdygdavll grcdieilkm etakrspter slkteirkya
      541 tsifnhvtkd qktaavvgde evmneystfl tpsikimdde nfdkndqvrl vvadnnkqav
      601 trasqiameh aftlchirik keklgddrie ktfnivnkwv yklwqhmain glacvifage
      661 nnagngvcfl nikrevpenc vira
//
LOCUS       XP_034189156             642 aa            linear   INV 03-SEP-2025
DEFINITION  RNA exonuclease 5 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034189156
VERSION     XP_034189156.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333265.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..642
                     /product="RNA exonuclease 5 isoform X2"
                     /calculated_mol_wt=72966
     Region          303..453
                     /region_name="REX1_like"
                     /note="DEDDh 3'-5' exonuclease domain of RNA exonuclease
                     1, -3 and similar eukaryotic proteins; cd06145"
                     /db_xref="CDD:99848"
     Site            order(306..309,311,385..386,388..390,439,444)
                     /site_type="active"
                     /db_xref="CDD:99848"
     Site            order(306..309,311,385..386,388..389,439,444)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:99848"
     Site            order(306,308,390,439,444)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99848"
     CDS             1..642
                     /gene="Rexo5"
                     /coded_by="XM_034333265.2:247..2175"
                     /db_xref="GeneID:117608317"
ORIGIN      
        1 msteidlsnd idnneismee synrkrpcde dlkgtcsgds tekedgeite eldplankkp
       61 rlsgeeylkl kqelrerkkr lksiprfhlk avgenasisi nvksedripi flsdiqhlll
      121 yslhghhspy lptrwchlek ynrvshtval vieglsiyhf mayesmfpsi tsnlehrvev
      181 vtpmiyggsv vedlaavpit gvhcdklieq ygslqsalns trdvikllkt vfpmyqtept
      241 dikrilelpy tdkfprtqll lslcqmveen ypiplsgela kkygnyimtk dvyleatars
      301 pmigldcemc kttmgelelt ritlvdekmn viydslvkpd npitdyltrf sgitkemled
      361 vtttlsdiqq vlrkllppda ilvgqslnsd lhtlkmmhpy iidtsvifni tgdryrktkl
      421 qtlvreflge kiqnstdghc saedsracmk lvqlklansv dygdavllgr cdieilkmet
      481 akrsptersl kteirkyats ifnhvtkdqk taavvgdeev mneystfltp sikimddenf
      541 dkndqvrlvv adnnkqavtr asqiamehaf tlchirikke klgddriekt fnivnkwvyk
      601 lwqhmaingl acvifagenn agngvcflni krevpencvi ra
//
LOCUS       XP_034189171             527 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189171
VERSION     XP_034189171.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333280.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189171.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..527
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X1"
                     /calculated_mol_wt=59162
     Region          144..274
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <326..400
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..527
                     /gene="axed"
                     /coded_by="XM_034333280.2:317..1900"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 miselfsyrd lmtselldcf acsvlwaase wivslafafc shlvnmrllt fadkrsagev
       61 gmrgnssrca rieseklrqr trqgarnrvy sktiifpmfl rhrskeigil enssqdmsqv
      121 plitntetgt tessasnmvi leagppgdtw rycvererla ekselframl vgplapapsd
      181 pppllqlqhv ekrafdhvlr ikkcpskvwl kirvqryrls gnclqlcrfl crylldkpin
      241 fqsvstarat ldaahqylnp dlarlaveyl esnlnsntvl eiyqgislya nhvtsglnqt
      301 stfgsptapp tpgddageia avctrllltc ldvidsdpea vfnqerfeel taveveqlac
      361 rdtlrltnes tlfsaldrwa asecrkqgve lssnnkrtal sdevwysvry plmsdrefie
      421 gpmasgilts eecafivtki lghtenpeee vrlllrnsvs gplsrlsstp rigvrtykdq
      481 sckmskpgkk ecqdnrknrr kecanqgqrt carignclvr vlacifd
//
LOCUS       XP_034189182             525 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189182
VERSION     XP_034189182.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333291.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189182.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X2"
                     /calculated_mol_wt=59020
     Region          144..274
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <324..398
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..525
                     /gene="axed"
                     /coded_by="XM_034333291.2:317..1894"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 miselfsyrd lmtselldcf acsvlwaase wivslafafc shlvnmrllt fadkrsagev
       61 gmrgnssrca rieseklrqr trqgarnrvy sktiifpmfl rhrskeigil enssqdmsqv
      121 plitntetgt tessasnmvi leagppgdtw rycvererla ekselframl vgplapapsd
      181 pppllqlqhv ekrafdhvlr ikkcpskvwl kirvqryrls gnclqlcrfl crylldkpin
      241 fqsvstarat ldaahqylnp dlarlaveyl esnlnsntvl eiyqgislya nhvtsglnqt
      301 stfgsptapp tpgddageiv ctrllltcld vidsdpeavf nqerfeelta veveqlacrd
      361 tlrltnestl fsaldrwaas ecrkqgvels snnkrtalsd evwysvrypl msdrefiegp
      421 masgiltsee cafivtkilg htenpeeevr lllrnsvsgp lsrlsstpri gvrtykdqsc
      481 kmskpgkkec qdnrknrrke canqgqrtca rignclvrvl acifd
//
LOCUS       XP_034189192             523 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189192
VERSION     XP_034189192.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333301.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189192.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..523
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X3"
                     /calculated_mol_wt=58680
     Region          144..274
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <326..400
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..523
                     /gene="axed"
                     /coded_by="XM_034333301.2:317..1888"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 miselfsyrd lmtselldcf acsvlwaase wivslafafc shlvnmrllt fadkrsagev
       61 gmrgnssrca rieseklrqr trqgarnrvy sktiifpmfl rhrskeigil enssqdmsqv
      121 plitntetgt tessasnmvi leagppgdtw rycvererla ekselframl vgplapapsd
      181 pppllqlqhv ekrafdhvlr ikkcpskvwl kirvqryrls gnclqlcrfl crylldkpin
      241 fqsvstarat ldaahqylnp dlarlaveyl esnlnsntvl eiyqgislya nhvtsglnqt
      301 stfgsptapp tpgddageia avctrllltc ldvidsdpea vfnqerfeel taveveqlac
      361 rdtlrltnes tlfsaldrwa asecrkqgve lssnnkrtal sdevwysvry plmsdrefie
      421 gpmasgilts eecafivtki lghtenpeee lrnsvsgpls rlsstprigv rtykdqsckm
      481 skpgkkecqd nrknrrkeca nqgqrtcari gnclvrvlac ifd
//
LOCUS       XP_034189202             495 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189202
VERSION     XP_034189202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X4"
                     /calculated_mol_wt=55270
     Region          144..242
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <294..368
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..495
                     /gene="axed"
                     /coded_by="XM_034333311.2:317..1804"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 miselfsyrd lmtselldcf acsvlwaase wivslafafc shlvnmrllt fadkrsagev
       61 gmrgnssrca rieseklrqr trqgarnrvy sktiifpmfl rhrskeigil enssqdmsqv
      121 plitntetgt tessasnmvi leagppgdtw rycvererla ekselframl vgplapapsd
      181 pppllqlqhv ekrafdhvlr ylldkpinfq svstaratld aahqylnpdl arlaveyles
      241 nlnsntvlei yqgislyanh vtsglnqtst fgsptapptp gddageiaav ctrllltcld
      301 vidsdpeavf nqerfeelta veveqlacrd tlrltnestl fsaldrwaas ecrkqgvels
      361 snnkrtalsd evwysvrypl msdrefiegp masgiltsee cafivtkilg htenpeeevr
      421 lllrnsvsgp lsrlsstpri gvrtykdqsc kmskpgkkec qdnrknrrke canqgqrtca
      481 rignclvrvl acifd
//
LOCUS       XP_034189238             491 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189238
VERSION     XP_034189238.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333347.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..491
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X5"
                     /calculated_mol_wt=54788
     Region          144..242
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <294..368
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..491
                     /gene="axed"
                     /coded_by="XM_034333347.2:317..1792"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 miselfsyrd lmtselldcf acsvlwaase wivslafafc shlvnmrllt fadkrsagev
       61 gmrgnssrca rieseklrqr trqgarnrvy sktiifpmfl rhrskeigil enssqdmsqv
      121 plitntetgt tessasnmvi leagppgdtw rycvererla ekselframl vgplapapsd
      181 pppllqlqhv ekrafdhvlr ylldkpinfq svstaratld aahqylnpdl arlaveyles
      241 nlnsntvlei yqgislyanh vtsglnqtst fgsptapptp gddageiaav ctrllltcld
      301 vidsdpeavf nqerfeelta veveqlacrd tlrltnestl fsaldrwaas ecrkqgvels
      361 snnkrtalsd evwysvrypl msdrefiegp masgiltsee cafivtkilg htenpeeelr
      421 nsvsgplsrl sstprigvrt ykdqsckmsk pgkkecqdnr knrrkecanq gqrtcarign
      481 clvrvlacif d
//
LOCUS       XP_034189210             471 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189210
VERSION     XP_034189210.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333319.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189210.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X6"
                     /calculated_mol_wt=52833
     Region          88..218
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <270..344
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..471
                     /gene="axed"
                     /coded_by="XM_034333319.2:867..2282"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 mtvayndgns srcariesek lrqrtrqgar nrvysktiif pmflrhrske igilenssqd
       61 msqvplitnt etgttessas nmvileagpp gdtwrycver erlaekself ramlvgplap
      121 apsdpppllq lqhvekrafd hvlrikkcps kvwlkirvqr yrlsgnclql crflcrylld
      181 kpinfqsvst aratldaahq ylnpdlarla veylesnlns ntvleiyqgi slyanhvtsg
      241 lnqtstfgsp tapptpgdda geiaavctrl lltcldvids dpeavfnqer feeltaveve
      301 qlacrdtlrl tnestlfsal drwaasecrk qgvelssnnk rtalsdevwy svryplmsdr
      361 efiegpmasg iltseecafi vtkilghten peeevrlllr nsvsgplsrl sstprigvrt
      421 ykdqsckmsk pgkkecqdnr knrrkecanq gqrtcarign clvrvlacif d
//
LOCUS       XP_034189220             430 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189220
VERSION     XP_034189220.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333329.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189220.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X7"
                     /calculated_mol_wt=48077
     Region          47..177
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <229..303
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..430
                     /gene="axed"
                     /coded_by="XM_034333329.2:540..1832"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 mflrhrskei gilenssqdm sqvplitnte tgttessasn mvileagppg dtwrycvere
       61 rlaekselfr amlvgplapa psdpppllql qhvekrafdh vlrikkcpsk vwlkirvqry
      121 rlsgnclqlc rflcrylldk pinfqsvsta ratldaahqy lnpdlarlav eylesnlnsn
      181 tvleiyqgis lyanhvtsgl nqtstfgspt apptpgddag eiaavctrll ltcldvidsd
      241 peavfnqerf eeltaveveq lacrdtlrlt nestlfsald rwaasecrkq gvelssnnkr
      301 talsdevwys vryplmsdre fiegpmasgi ltseecafiv tkilghtenp eeevrlllrn
      361 svsgplsrls stprigvrty kdqsckmskp gkkecqdnrk nrrkecanqg qrtcarignc
      421 lvrvlacifd
//
LOCUS       XP_034189229             430 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein axundead isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_034189229
VERSION     XP_034189229.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189229.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="BTB/POZ domain-containing protein axundead
                     isoform X7"
                     /calculated_mol_wt=48077
     Region          47..177
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     Region          <229..303
                     /region_name="BACK"
                     /note="BACK (BTB and C-terminal Kelch) domain; cl28903"
                     /db_xref="CDD:475122"
     CDS             1..430
                     /gene="axed"
                     /coded_by="XM_034333338.2:636..1928"
                     /db_xref="GeneID:117608328"
ORIGIN      
        1 mflrhrskei gilenssqdm sqvplitnte tgttessasn mvileagppg dtwrycvere
       61 rlaekselfr amlvgplapa psdpppllql qhvekrafdh vlrikkcpsk vwlkirvqry
      121 rlsgnclqlc rflcrylldk pinfqsvsta ratldaahqy lnpdlarlav eylesnlnsn
      181 tvleiyqgis lyanhvtsgl nqtstfgspt apptpgddag eiaavctrll ltcldvidsd
      241 peavfnqerf eeltaveveq lacrdtlrlt nestlfsald rwaasecrkq gvelssnnkr
      301 talsdevwys vryplmsdre fiegpmasgi ltseecafiv tkilghtenp eeevrlllrn
      361 svsgplsrls stprigvrty kdqsckmskp gkkecqdnrk nrrkecanqg qrtcarignc
      421 lvrvlacifd
//
LOCUS       XP_034189253             173 aa            linear   INV 03-SEP-2025
DEFINITION  basic helix-loop-helix domain-containing transcription factor
            HLH54F [Osmia lignaria lignaria].
ACCESSION   XP_034189253
VERSION     XP_034189253.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333362.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..173
                     /product="basic helix-loop-helix domain-containing
                     transcription factor HLH54F"
                     /calculated_mol_wt=19415
     Region          36..90
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(43..44,46,51,71..72)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381392"
     Site            order(49..50,53..54,56..57,61,72..73,76..77,80,82..83,
                     86..87)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381392"
     CDS             1..173
                     /gene="HLH54F"
                     /coded_by="XM_034333362.2:227..748"
                     /db_xref="GeneID:117608349"
ORIGIN      
        1 mprkrrastp mleadeeefg seekyskdel dnraprnaan arerarmrvl skafcrlktt
       61 lpwvpadtkl skldtlrlaa tyiahlravl rddgdahset trslslalsw pfalqgsnas
      121 mlmnnscnvs sstssssnqy rgqqgtheip nfhhpghqsm smrhnhepql syf
//
LOCUS       XP_034189261             173 aa            linear   INV 03-SEP-2025
DEFINITION  basic helix-loop-helix domain-containing transcription factor
            HLH54F [Osmia lignaria lignaria].
ACCESSION   XP_034189261
VERSION     XP_034189261.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333370.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..173
                     /product="basic helix-loop-helix domain-containing
                     transcription factor HLH54F"
                     /calculated_mol_wt=19415
     Region          36..90
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(43..44,46,51,71..72)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381392"
     Site            order(49..50,53..54,56..57,61,72..73,76..77,80,82..83,
                     86..87)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381392"
     CDS             1..173
                     /gene="HLH54F"
                     /coded_by="XM_034333370.2:270..791"
                     /db_xref="GeneID:117608349"
ORIGIN      
        1 mprkrrastp mleadeeefg seekyskdel dnraprnaan arerarmrvl skafcrlktt
       61 lpwvpadtkl skldtlrlaa tyiahlravl rddgdahset trslslalsw pfalqgsnas
      121 mlmnnscnvs sstssssnqy rgqqgtheip nfhhpghqsm smrhnhepql syf
//
LOCUS       XP_034181521             993 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate receptor IB isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181521
VERSION     XP_034181521.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325630.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..993
                     /product="glutamate receptor IB isoform X1"
                     /calculated_mol_wt=111691
     Region          73..469
                     /region_name="PBP1_iGluR_AMPA"
                     /note="N-terminal leucine-isoleucine-valine binding
                     protein (LIVBP)-like domain of the AMPA receptor; cd06380"
                     /db_xref="CDD:380603"
     Site            order(117..119,122..123,126,151,155,206,212,216,219..220,
                     223,225,233..234,236,252,400..402,405..407)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Site            order(117..119,122..123,126,151,206,216,219..220,223,225,
                     234,236,252,399..401,405,407)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Region          487..892
                     /region_name="PBP2_iGluR_AMPA"
                     /note="The ligand-binding domain of the AMPA
                     (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic
                     acid) subtypes of ionotropic glutamate receptors, a member
                     of the type 2 periplasmic binding fold protein
                     superfamily; cd13715"
                     /db_xref="CDD:270433"
     Site            order(528,532,580..581,584,589..592,848,866..867,870..875,
                     877,880)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(547,576..578,583,770,773..775,823..824,827,851)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(591..595,870,873,878..879,882)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:270433"
     Region          620..915
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..993
                     /gene="GluRIB"
                     /coded_by="XM_034325630.2:69..3050"
                     /db_xref="GeneID:117604968"
ORIGIN      
        1 mlpvvhetgg pplsrrrrra tifhgvvsnt lensrnvgss ritrplstft vmttafsilm
       61 vllpaaypdk isigaifeqg tdevqsafkf amvnhnqntt trkfellafv dvintadayk
      121 lsrlicsqfs rgvfsmlgav spdsfdtlhs ysntfqmpfv tpwfpekvlt pssglldfai
      181 smrpdyhrai idtvryygwk kiiylydshd gllrlqqiyq glkpgnesfq vetvkriqnm
      241 seaidflrsl eelnrwsnky vvldcptdma kdivvshvrd valgkrtyhy llsglimddr
      301 wesevieyga initgfrivd atrpyvkdfl aswhrldpan sqgagresis aqaalmydav
      361 fvlveafnkf lrkkpdrsnv rrtgipgsnq itngtraldc nssrgwvtpf eygdkisrll
      421 rkveiegltg eirfnddgrr hnytlhvvem tvnsamvkva ewtdeagfqa iaakyirlrp
      481 haeieknkty ivttiveepy imkkksenge vltgndsyeg yckdladlia kklgityelr
      541 ivkdgkygme npdvpggwdg mvgelirkea diaiapmtit serervidfs kpfmslgisi
      601 mikkpikqkp gvfsflnpls keiwvcvifs yigvsivlft vsrfspyewr vltlssggdp
      661 tmgtrndptl qhphgsqgsp hmptssmand fsiinslwfa laafmqqgcd isprsisgri
      721 vgsvwwfftl ilissytanl aafltvermv apinspedla sqtevqygtl shgstwdffr
      781 ksqinlyskm wefmnsrkhv fvktydegir rvrtskgkya lliespkney inerepcdtm
      841 kvgrnldakg fgvatplgsp lrdpinlavl slkengeltk lvnrwwydrt ecrhgdkqda
      901 srnelslsnv agifyiligg lllalavall efcykshtea trakiplsda mkakarltig
      961 ggrdfdngry yapanaignt egdqvhsnth tqv
//
LOCUS       XP_034181529             975 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate receptor IB isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181529
VERSION     XP_034181529.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325638.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..975
                     /product="glutamate receptor IB isoform X2"
                     /calculated_mol_wt=109856
     Region          73..469
                     /region_name="PBP1_iGluR_AMPA"
                     /note="N-terminal leucine-isoleucine-valine binding
                     protein (LIVBP)-like domain of the AMPA receptor; cd06380"
                     /db_xref="CDD:380603"
     Site            order(117..119,122..123,126,151,155,206,212,216,219..220,
                     223,225,233..234,236,252,400..402,405..407)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Site            order(117..119,122..123,126,151,206,216,219..220,223,225,
                     234,236,252,399..401,405,407)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Region          487..892
                     /region_name="PBP2_iGluR_AMPA"
                     /note="The ligand-binding domain of the AMPA
                     (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic
                     acid) subtypes of ionotropic glutamate receptors, a member
                     of the type 2 periplasmic binding fold protein
                     superfamily; cd13715"
                     /db_xref="CDD:270433"
     Site            order(528,532,580..581,584,589..592,848,866..867,870..875,
                     877,880)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(547,576..578,583,770,773..775,823..824,827,851)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(591..595,870,873,878..879,882)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:270433"
     Region          620..915
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..975
                     /gene="GluRIB"
                     /coded_by="XM_034325638.2:69..2996"
                     /db_xref="GeneID:117604968"
ORIGIN      
        1 mlpvvhetgg pplsrrrrra tifhgvvsnt lensrnvgss ritrplstft vmttafsilm
       61 vllpaaypdk isigaifeqg tdevqsafkf amvnhnqntt trkfellafv dvintadayk
      121 lsrlicsqfs rgvfsmlgav spdsfdtlhs ysntfqmpfv tpwfpekvlt pssglldfai
      181 smrpdyhrai idtvryygwk kiiylydshd gllrlqqiyq glkpgnesfq vetvkriqnm
      241 seaidflrsl eelnrwsnky vvldcptdma kdivvshvrd valgkrtyhy llsglimddr
      301 wesevieyga initgfrivd atrpyvkdfl aswhrldpan sqgagresis aqaalmydav
      361 fvlveafnkf lrkkpdrsnv rrtgipgsnq itngtraldc nssrgwvtpf eygdkisrll
      421 rkveiegltg eirfnddgrr hnytlhvvem tvnsamvkva ewtdeagfqa iaakyirlrp
      481 haeieknkty ivttiveepy imkkksenge vltgndsyeg yckdladlia kklgityelr
      541 ivkdgkygme npdvpggwdg mvgelirkea diaiapmtit serervidfs kpfmslgisi
      601 mikkpikqkp gvfsflnpls keiwvcvifs yigvsivlft vsrfspyewr vltlssggdp
      661 tmgtrndptl qhphgsqgsp hmptssmand fsiinslwfa laafmqqgcd isprsisgri
      721 vgsvwwfftl ilissytanl aafltvermv apinspedla sqtevqygtl shgstwdffr
      781 ksqinlyskm wefmnsrkhv fvktydegir rvrtskgkya lliespkney inerepcdtm
      841 kvgrnldakg fgvatplgsp lrdpinlavl slkengeltk lvnrwwydrt ecrhgdkqda
      901 srnelslsnv agifyiligg lllalavall efcykshtea trakiplsda mkakarltig
      961 ggrdfdngrw yglqs
//
LOCUS       XP_034181542             942 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate receptor IB isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034181542
VERSION     XP_034181542.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325651.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..942
                     /product="glutamate receptor IB isoform X3"
                     /calculated_mol_wt=106051
     Region          22..418
                     /region_name="PBP1_iGluR_AMPA"
                     /note="N-terminal leucine-isoleucine-valine binding
                     protein (LIVBP)-like domain of the AMPA receptor; cd06380"
                     /db_xref="CDD:380603"
     Site            order(66..68,71..72,75,100,104,155,161,165,168..169,172,
                     174,182..183,185,201,349..351,354..356)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Site            order(66..68,71..72,75,100,155,165,168..169,172,174,183,
                     185,201,348..350,354,356)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Region          436..841
                     /region_name="PBP2_iGluR_AMPA"
                     /note="The ligand-binding domain of the AMPA
                     (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic
                     acid) subtypes of ionotropic glutamate receptors, a member
                     of the type 2 periplasmic binding fold protein
                     superfamily; cd13715"
                     /db_xref="CDD:270433"
     Site            order(477,481,529..530,533,538..541,797,815..816,819..824,
                     826,829)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(496,525..527,532,719,722..724,772..773,776,800)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(540..544,819,822,827..828,831)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:270433"
     Region          569..864
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..942
                     /gene="GluRIB"
                     /coded_by="XM_034325651.2:324..3152"
                     /db_xref="GeneID:117604968"
ORIGIN      
        1 mttafsilmv llpaaypdki sigaifeqgt devqsafkfa mvnhnqnttt rkfellafvd
       61 vintadaykl srlicsqfsr gvfsmlgavs pdsfdtlhsy sntfqmpfvt pwfpekvltp
      121 ssglldfais mrpdyhraii dtvryygwkk iiylydshdg llrlqqiyqg lkpgnesfqv
      181 etvkriqnms eaidflrsle elnrwsnkyv vldcptdmak divvshvrdv algkrtyhyl
      241 lsglimddrw esevieygai nitgfrivda trpyvkdfla swhrldpans qgagresisa
      301 qaalmydavf vlveafnkfl rkkpdrsnvr rtgipgsnqi tngtraldcn ssrgwvtpfe
      361 ygdkisrllr kveiegltge irfnddgrrh nytlhvvemt vnsamvkvae wtdeagfqai
      421 aakyirlrph aeieknktyi vttiveepyi mkkksengev ltgndsyegy ckdladliak
      481 klgityelri vkdgkygmen pdvpggwdgm vgelirkead iaiapmtits erervidfsk
      541 pfmslgisim ikkpikqkpg vfsflnplsk eiwvcvifsy igvsivlftv srfspyewrv
      601 ltlssggdpt mgtrndptlq hphgsqgsph mptssmandf siinslwfal aafmqqgcdi
      661 sprsisgriv gsvwwfftli lissytanla afltvermva pinspedlas qtevqygtls
      721 hgstwdffrk sqinlyskmw efmnsrkhvf vktydegirr vrtskgkyal liespkneyi
      781 nerepcdtmk vgrnldakgf gvatplgspl rdpinlavls lkengeltkl vnrwwydrte
      841 crhgdkqdas rnelslsnva gifyiliggl llalavalle fcykshteat rakiplsdam
      901 kakarltigg grdfdngryy apanaignte gdqvhsntht qv
//
LOCUS       XP_034181550             902 aa            linear   INV 03-SEP-2025
DEFINITION  glutamate receptor IB isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034181550
VERSION     XP_034181550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325659.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..902
                     /product="glutamate receptor IB isoform X4"
                     /calculated_mol_wt=101746
     Region          1..378
                     /region_name="PBP1_iGluR_AMPA"
                     /note="N-terminal leucine-isoleucine-valine binding
                     protein (LIVBP)-like domain of the AMPA receptor; cd06380"
                     /db_xref="CDD:380603"
     Site            order(26..28,31..32,35,60,64,115,121,125,128..129,132,134,
                     142..143,145,161,309..311,314..316)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Site            order(26..28,31..32,35,60,115,125,128..129,132,134,143,
                     145,161,308..310,314,316)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:380603"
     Region          396..801
                     /region_name="PBP2_iGluR_AMPA"
                     /note="The ligand-binding domain of the AMPA
                     (alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionic
                     acid) subtypes of ionotropic glutamate receptors, a member
                     of the type 2 periplasmic binding fold protein
                     superfamily; cd13715"
                     /db_xref="CDD:270433"
     Site            order(437,441,489..490,493,498..501,757,775..776,779..784,
                     786,789)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(456,485..487,492,679,682..684,732..733,736,760)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:270433"
     Site            order(500..504,779,782,787..788,791)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:270433"
     Region          529..824
                     /region_name="Lig_chan"
                     /note="Ligand-gated ion channel; pfam00060"
                     /db_xref="CDD:459656"
     CDS             1..902
                     /gene="GluRIB"
                     /coded_by="XM_034325659.2:325..3033"
                     /db_xref="GeneID:117604968"
ORIGIN      
        1 mvnhnqnttt rkfellafvd vintadaykl srlicsqfsr gvfsmlgavs pdsfdtlhsy
       61 sntfqmpfvt pwfpekvltp ssglldfais mrpdyhraii dtvryygwkk iiylydshdg
      121 llrlqqiyqg lkpgnesfqv etvkriqnms eaidflrsle elnrwsnkyv vldcptdmak
      181 divvshvrdv algkrtyhyl lsglimddrw esevieygai nitgfrivda trpyvkdfla
      241 swhrldpans qgagresisa qaalmydavf vlveafnkfl rkkpdrsnvr rtgipgsnqi
      301 tngtraldcn ssrgwvtpfe ygdkisrllr kveiegltge irfnddgrrh nytlhvvemt
      361 vnsamvkvae wtdeagfqai aakyirlrph aeieknktyi vttiveepyi mkkksengev
      421 ltgndsyegy ckdladliak klgityelri vkdgkygmen pdvpggwdgm vgelirkead
      481 iaiapmtits erervidfsk pfmslgisim ikkpikqkpg vfsflnplsk eiwvcvifsy
      541 igvsivlftv srfspyewrv ltlssggdpt mgtrndptlq hphgsqgsph mptssmandf
      601 siinslwfal aafmqqgcdi sprsisgriv gsvwwfftli lissytanla afltvermva
      661 pinspedlas qtevqygtls hgstwdffrk sqinlyskmw efmnsrkhvf vktydegirr
      721 vrtskgkyal liespkneyi nerepcdtmk vgrnldakgf gvatplgspl rdpinlavls
      781 lkengeltkl vnrwwydrte crhgdkqdas rnelslsnva gifyiliggl llalavalle
      841 fcykshteat rakiplsdam kakarltigg grdfdngryy apanaignte gdqvhsntht
      901 qv
//
LOCUS       XP_034181602             484 aa            linear   INV 03-SEP-2025
DEFINITION  resistant to dieldrin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181602
VERSION     XP_034181602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="resistant to dieldrin isoform X1"
                     /calculated_mol_wt=54293
     Region          55..466
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          69..252
                     /region_name="LGIC_ECD_GABAR_RDL-like"
                     /note="gamma-aminobutyric acid receptor subunit beta-like
                     extracellular domain in protostomia, such as RDL
                     (resistant to dieldrin); cd19008"
                     /db_xref="CDD:349809"
     Site            order(76,95)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:349809"
     Site            order(79,81..82,87..88,95,97..98,117,119..120,129..134,
                     137,139,141..142,148..150,152,162..164,192,252)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349809"
     Region          171..185
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349809"
     CDS             1..484
                     /gene="Rdl"
                     /coded_by="XM_034325711.2:220..1674"
                     /db_xref="GeneID:117605003"
ORIGIN      
        1 mksfhaasws fallaatval lpathrapfa qaatgggsml gdvnisaild afsvnydkrv
       61 rpnyggppve vgvtmyvlsi sslsevkmdf tldfyfrqfw tdprlafkkr kgvetlsvgs
      121 efikniwvpd tffvnekqsy fhiattsnef irihhsgsit rsirltitas cpmnlqyfpm
      181 drqlchieie sfgytmrdir ykwnegpnsv gvsnevslpq fkvlghrqra meislttgny
      241 srlaceiqfv rsmgyyliqi yipsglivii swvsfwlnrn atparvalgv ttvltmttlm
      301 sstnaalpki syvksidvyl gtcfvmvfas lleyatvgym akriqmrknr fqkiaesmkt
      361 arenpgppgv pgdhgdhapk qtwsrvvqev rfkvhdpkah skggtlenti ngradeeaap
      421 apqhlihpgk disklygmtp sdidkysriv fpvcfvcfnl mywiiylhis dvvaddlvll
      481 eeak
//
LOCUS       XP_034181623             484 aa            linear   INV 03-SEP-2025
DEFINITION  resistant to dieldrin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181623
VERSION     XP_034181623.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325732.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="resistant to dieldrin isoform X2"
                     /calculated_mol_wt=54379
     Region          55..466
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          69..252
                     /region_name="LGIC_ECD_GABAR_RDL-like"
                     /note="gamma-aminobutyric acid receptor subunit beta-like
                     extracellular domain in protostomia, such as RDL
                     (resistant to dieldrin); cd19008"
                     /db_xref="CDD:349809"
     Site            order(76,95)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:349809"
     Site            order(79,81..82,87..88,95,97..98,117,119..120,129..134,
                     137,139,141..142,148..150,152,162..164,192,252)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349809"
     Region          171..185
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349809"
     CDS             1..484
                     /gene="Rdl"
                     /coded_by="XM_034325732.2:220..1674"
                     /db_xref="GeneID:117605003"
ORIGIN      
        1 mksfhaasws fallaatval lpathrapfa qaatgggsml gdvnisaild afsvnydkrv
       61 rpnyggppve vgvtmyvlsi sslsevkmdf tldfyfrqfw tdprlafkkr kgvetlsvgs
      121 efikniwvpd tffvnekqsy fhiattsnef irihhsgsit rsirltitas cpmnlqyfpm
      181 drqlchieie sfgytmrdir ykwnaglqsv gisnevelpq frvlghrqrh stihlstgny
      241 srlaceiqfv rsmgyyliqi yipsglivii swvsfwlnrn atparvalgv ttvltmttlm
      301 sstnaalpki syvksidvyl gtcfvmvfas lleyatvgym akriqmrknr fqkiaesmkt
      361 arenpgppgv pgdhgdhapk qtwsrvvqev rfkvhdpkah skggtlenti ngradeeaap
      421 apqhlihpgk disklygmtp sdidkysriv fpvcfvcfnl mywiiylhis dvvaddlvll
      481 eeak
//
LOCUS       XP_034181633             478 aa            linear   INV 03-SEP-2025
DEFINITION  resistant to dieldrin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034181633
VERSION     XP_034181633.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325742.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="resistant to dieldrin isoform X3"
                     /calculated_mol_wt=53537
     Region          55..460
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          69..252
                     /region_name="LGIC_ECD_GABAR_RDL-like"
                     /note="gamma-aminobutyric acid receptor subunit beta-like
                     extracellular domain in protostomia, such as RDL
                     (resistant to dieldrin); cd19008"
                     /db_xref="CDD:349809"
     Site            order(76,95)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:349809"
     Site            order(79,81..82,87..88,95,97..98,117,119..120,129..134,
                     137,139,141..142,148..150,152,162..164,192,252)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349809"
     Region          171..185
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349809"
     CDS             1..478
                     /gene="Rdl"
                     /coded_by="XM_034325742.2:220..1656"
                     /db_xref="GeneID:117605003"
ORIGIN      
        1 mksfhaasws fallaatval lpathrapfa qaatgggsml gdvnisaild afsvnydkrv
       61 rpnyggppve vgvtmyvlsi sslsevkmdf tldfyfrqfw tdprlafkkr kgvetlsvgs
      121 efikniwvpd tffvnekqsy fhiattsnef irihhsgsit rsirltitas cpmnlqyfpm
      181 drqlchieie sfgytmrdir ykwnegpnsv gvsnevslpq fkvlghrqra meislttgny
      241 srlaceiqfv rsmgyyliqi yipsglivii swvsfwlnrn atparvalgv ttvltmttlm
      301 sstnaalpki syvksidvyl gtcfvmvfas lleyatvgym akriqmrknr fqkiaesmkt
      361 arenpgppgv pgdhgdhapk qtevrfkvhd pkahskggtl entingrade eaapapqhli
      421 hpgkdiskly gmtpsdidky srivfpvcfv cfnlmywiiy lhisdvvadd lvlleeak
//
LOCUS       XP_034181643             478 aa            linear   INV 03-SEP-2025
DEFINITION  resistant to dieldrin isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034181643
VERSION     XP_034181643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325752.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="resistant to dieldrin isoform X4"
                     /calculated_mol_wt=53508
     Region          55..460
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          69..252
                     /region_name="LGIC_ECD_GABAR_RDL-like"
                     /note="gamma-aminobutyric acid receptor subunit beta-like
                     extracellular domain in protostomia, such as RDL
                     (resistant to dieldrin); cd19008"
                     /db_xref="CDD:349809"
     Site            order(76,95)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:349809"
     Site            order(79,81..82,87..88,95,97..98,117,119..120,129..134,
                     137,139,141..142,148..150,152,162..164,192,252)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349809"
     Region          171..185
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349809"
     CDS             1..478
                     /gene="Rdl"
                     /coded_by="XM_034325752.2:220..1656"
                     /db_xref="GeneID:117605003"
ORIGIN      
        1 mksfhaasws fallaatval lpathrapfa qaatgggsml gdvnisaild afsvnydkrv
       61 rpnyggppve vgvtmyvlsi ssvsevlmdf tldfyfrqfw tdprlafkkr kgvetlsvgs
      121 efikniwvpd tffvnekqsy fhiattsnef irihhsgsit rsirltitas cpmnlqyfpm
      181 drqlchieie sfgytmrdir ykwnegpnsv gvsnevslpq fkvlghrqra meislttgny
      241 srlaceiqfv rsmgyyliqi yipsglivii swvsfwlnrn atparvalgv ttvltmttlm
      301 sstnaalpki syvksidvyl gtcfvmvfas lleyatvgym akriqmrknr fqkiaesmkt
      361 arenpgppgv pgdhgdhapk qtevrfkvhd pkahskggtl entingrade eaapapqhli
      421 hpgkdiskly gmtpsdidky srivfpvcfv cfnlmywiiy lhisdvvadd lvlleeak
//
LOCUS       XP_034181653             477 aa            linear   INV 03-SEP-2025
DEFINITION  resistant to dieldrin isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034181653
VERSION     XP_034181653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325762.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="resistant to dieldrin isoform X5"
                     /calculated_mol_wt=53408
     Region          55..459
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          69..252
                     /region_name="LGIC_ECD_GABAR_RDL-like"
                     /note="gamma-aminobutyric acid receptor subunit beta-like
                     extracellular domain in protostomia, such as RDL
                     (resistant to dieldrin); cd19008"
                     /db_xref="CDD:349809"
     Site            order(76,95)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:349809"
     Site            order(79,81..82,87..88,95,97..98,117,119..120,129..134,
                     137,139,141..142,148..150,152,162..164,192,252)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349809"
     Region          171..185
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349809"
     CDS             1..477
                     /gene="Rdl"
                     /coded_by="XM_034325762.2:220..1653"
                     /db_xref="GeneID:117605003"
ORIGIN      
        1 mksfhaasws fallaatval lpathrapfa qaatgggsml gdvnisaild afsvnydkrv
       61 rpnyggppve vgvtmyvlsi sslsevkmdf tldfyfrqfw tdprlafkkr kgvetlsvgs
      121 efikniwvpd tffvnekqsy fhiattsnef irihhsgsit rsirltitas cpmnlqyfpm
      181 drqlchieie sfgytmrdir ykwnegpnsv gvsnevslpq fkvlghrqra meislttgny
      241 srlaceiqfv rsmgyyliqi yipsglivii swvsfwlnrn atparvalgv ttvltmttlm
      301 sstnaalpki syvksidvyl gtcfvmvfas lleyatvgym akriqmrknr fqkiaesmkt
      361 arenpgppgv pgdhgdhapk qtvrfkvhdp kahskggtle ntingradee aapapqhlih
      421 pgkdisklyg mtpsdidkys rivfpvcfvc fnlmywiiyl hisdvvaddl vlleeak
//
LOCUS       XP_034181667             477 aa            linear   INV 03-SEP-2025
DEFINITION  resistant to dieldrin isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034181667
VERSION     XP_034181667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="resistant to dieldrin isoform X6"
                     /calculated_mol_wt=53494
     Region          55..459
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          69..252
                     /region_name="LGIC_ECD_GABAR_RDL-like"
                     /note="gamma-aminobutyric acid receptor subunit beta-like
                     extracellular domain in protostomia, such as RDL
                     (resistant to dieldrin); cd19008"
                     /db_xref="CDD:349809"
     Site            order(76,95)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:349809"
     Site            order(79,81..82,87..88,95,97..98,117,119..120,129..134,
                     137,139,141..142,148..150,152,162..164,192,252)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349809"
     Region          171..185
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349809"
     CDS             1..477
                     /gene="Rdl"
                     /coded_by="XM_034325776.2:220..1653"
                     /db_xref="GeneID:117605003"
ORIGIN      
        1 mksfhaasws fallaatval lpathrapfa qaatgggsml gdvnisaild afsvnydkrv
       61 rpnyggppve vgvtmyvlsi sslsevkmdf tldfyfrqfw tdprlafkkr kgvetlsvgs
      121 efikniwvpd tffvnekqsy fhiattsnef irihhsgsit rsirltitas cpmnlqyfpm
      181 drqlchieie sfgytmrdir ykwnaglqsv gisnevelpq frvlghrqrh stihlstgny
      241 srlaceiqfv rsmgyyliqi yipsglivii swvsfwlnrn atparvalgv ttvltmttlm
      301 sstnaalpki syvksidvyl gtcfvmvfas lleyatvgym akriqmrknr fqkiaesmkt
      361 arenpgppgv pgdhgdhapk qtvrfkvhdp kahskggtle ntingradee aapapqhlih
      421 pgkdisklyg mtpsdidkys rivfpvcfvc fnlmywiiyl hisdvvaddl vlleeak
//
LOCUS       XP_076546414             409 aa            linear   INV 03-SEP-2025
DEFINITION  resistant to dieldrin isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546414
VERSION     XP_076546414.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690299.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="resistant to dieldrin isoform X7"
                     /calculated_mol_wt=46103
     Region          <118..391
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     CDS             1..409
                     /gene="Rdl"
                     /coded_by="XM_076690299.1:3964..5193"
                     /db_xref="GeneID:117605003"
ORIGIN      
        1 mntkvtichc hrittttttt ttttnhhyhn tkplapfeik iykpptyste prvrgrskgr
       61 rrpapkeakt qkttcrlrcd rikthcassp ahvknettaa fshevtpwph ppptpfvgyt
      121 mrdirykwna glqsvgisne velpqfrvlg hrqrhstihl stgnysrlac eiqfvrsmgy
      181 yliqiyipsg liviiswvsf wlnrnatpar valgvttvlt mttlmsstna alpkisyvks
      241 idvylgtcfv mvfaslleya tvgymakriq mrknrfqkia esmktarenp gppgvpgdhg
      301 dhapkqtwsr vvqevrfkvh dpkahskggt lentingrad eeaapapqhl ihpgkdiskl
      361 ygmtpsdidk ysrivfpvcf vcfnlmywii ylhisdvvad dlvlleeak
//
LOCUS       XP_034181689             454 aa            linear   INV 03-SEP-2025
DEFINITION  trehalose transporter 1-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034181689
VERSION     XP_034181689.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325798.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..454
                     /product="trehalose transporter 1-like protein"
                     /calculated_mol_wt=50185
     Region          13..445
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(22..23,26..27,30,64,114..115,117..119,122,142,
                     145..146,149,257,260..261,264..266,269,296,300,361..362,
                     366,370,386,389..390,393..394,397)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..454
                     /gene="LOC117605026"
                     /coded_by="XM_034325798.2:904..2268"
                     /db_xref="GeneID:117605026"
ORIGIN      
        1 mdlgilrqmf igiicnllvi dsglnegwit piipkfqqdd plrvstdqmv wvvnlmyvgv
       61 glgslvpfll mdrigrkgtl lfatipkiss wfliglansi pqlyagrila gvgcgityav
      121 mpmylgevss krtrgplgtl mavllntgml lvyaiglwvs rftmsmisvv ipilflvmfv
      181 wlpessvflt kkkkltsaer tlkwtlgked vveeleeikr ivatedlkpd vtfagslkem
      241 ftkrqnlkaf rialivlsal tltgaaplla yqsfifeeag fdistnisiv itgcaivlag
      301 tvcvalvrit gkrllllisa picvlslmim giffgllsrg edvsnlrwvp svflviyvlg
      361 yglalnpipl ayigeifhid vkvpaaifcs lyyaittttv vkfyqvlqes ygtympmfaf
      421 taitafvwll iyryvpeteg ktleeiqtel kskn
//
LOCUS       XP_034181702             285 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor IIEbeta [Osmia lignaria lignaria].
ACCESSION   XP_034181702
VERSION     XP_034181702.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325811.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="transcription factor IIEbeta"
                     /calculated_mol_wt=32902
     Region          63..142
                     /region_name="TFIIE_beta_winged_helix"
                     /note="TFIIE_beta_winged_helix domain, located at the
                     central core region of TFIIE beta, with double-stranded
                     DNA binding activity; cd07977"
                     /db_xref="CDD:153423"
     Region          142..201
                     /region_name="TFA2_Winged_2"
                     /note="TFA2 Winged helix domain 2; pfam18121"
                     /db_xref="CDD:465658"
     CDS             1..285
                     /gene="TfIIEbeta"
                     /coded_by="XM_034325811.2:143..1000"
                     /db_xref="GeneID:117605032"
ORIGIN      
        1 mdpallrere lfkkralstp avekkkkeqd kdsardeplk kkpkpssvst gpkldmvnyk
       61 tmsgstqykf gvlakivkhm ktrhqdgddh pltleeilde tnqldvgskv kqwlqteali
      121 knpkievtsd grfvfkamyk ikdkksllrl lkqqdlkglg gillediqes lphcdkhlks
      181 lqneilfitr pmdkkkivfy ndktaqfpid defqklwrav avdamddqki deylekqgir
      241 smqdhgpkkp apikrkkpvg krkqfkrprd nehladvlet yddtk
//
LOCUS       XP_034181715             216 aa            linear   INV 03-SEP-2025
DEFINITION  ras-related and estrogen-regulated growth inhibitor isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034181715
VERSION     XP_034181715.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325824.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..216
                     /product="ras-related and estrogen-regulated growth
                     inhibitor isoform X1"
                     /calculated_mol_wt=23990
     Region          20..180
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            25..32
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206713"
     Site            order(27..33,75,131..132,134,161..163)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206713"
     Site            50
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206713"
     Site            53..55
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206713"
     Site            72..75
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206713"
     Site            order(74..75,91..92)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206713"
     Site            131..134
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206713"
     Site            161..163
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206713"
     CDS             1..216
                     /gene="LOC117605037"
                     /coded_by="XM_034325824.2:382..1032"
                     /db_xref="GeneID:117605037"
ORIGIN      
        1 mtsnairgir rkksslcevk vavigapgvg ksaltvrflt rryigeydhq senrykhevl
       61 vdgepilfei ldtcpksede lpstetlqwa dglllvysit drgsfnfvrk aketlavadp
      121 eatmplalvg nkadmvhlrq vsteegeila kdfecwfsev saaeqvtqva esfhelcrev
      181 laarrrnkqs lldrmlgska traysrgksd salpkd
//
LOCUS       XP_034181725             197 aa            linear   INV 03-SEP-2025
DEFINITION  ras-related and estrogen-regulated growth inhibitor isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034181725
VERSION     XP_034181725.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325834.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..197
                     /product="ras-related and estrogen-regulated growth
                     inhibitor isoform X2"
                     /calculated_mol_wt=21683
     Region          20..161
                     /region_name="P-loop containing Nucleoside Triphosphate
                     Hydrolases"
                     /note="Members of the P-loop NTPase domain superfamily are
                     characterized by a conserved nucleotide phosphate-binding
                     motif, also referred to as the Walker A motif
                     (GxxxxGK[S/T], where x is any residue), and the Walker B
                     motif (hhhh[D/E], where h is a...; cl38936"
                     /db_xref="CDD:476819"
     Site            25..32
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            order(27..32,56,112..113,115,142..144)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206648"
     Site            34..36
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206648"
     Site            53..56
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(55..56,72..73)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            112..115
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206648"
     Site            142..144
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206648"
     CDS             1..197
                     /gene="LOC117605037"
                     /coded_by="XM_034325834.2:383..976"
                     /db_xref="GeneID:117605037"
ORIGIN      
        1 mtsnairgir rkksslcevk vavigapgvg ksenrykhev lvdgepilfe ildtcpksed
       61 elpstetlqw adglllvysi tdrgsfnfvr kaketlavad peatmplalv gnkadmvhlr
      121 qvsteegeil akdfecwfse vsaaeqvtqv aesfhelcre vlaarrrnkq slldrmlgsk
      181 atraysrgks dsalpkd
//
LOCUS       XP_034185166             643 aa            linear   INV 03-SEP-2025
DEFINITION  pipsqueak isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034185166
VERSION     XP_034185166.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329275.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..643
                     /product="pipsqueak isoform X1"
                     /calculated_mol_wt=71158
     Region          44..128
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          403..>438
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          457..495
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          512..557
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          564..606
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..643
                     /gene="Psq"
                     /coded_by="XM_034329275.2:116..2047"
                     /db_xref="GeneID:117606583"
ORIGIN      
        1 metdrywgvh ysggmagqhy clrwnnyqsn mtsvfhqllq teafvdvtla cneaslkahk
       61 vvlsacssyf qklllsnpck hptiimpqdv cfndlkfiie fvyrgeidvs qaelqsllkt
      121 adqlkikglc evpesrdgpp svslssppre pgtprinftk lkrhhprykr artsfeprat
      181 dsrhydryke eesnenynre nkenhrdwqa edeectegtt aavaletcqr nnnnnninns
      241 nnnnnngsns snsngtnnnn gdmfchtglg hyghhpdpgd vdlppetqpt ppsatlvgtt
      301 ithlrdpdhh ateiqncdsv kikfetlhtm dssdtidids hmsdrasvss knaadndnmm
      361 mitpellglm psgssvhsds gennsrghsg qssshhhgsk swtqedmdaa lealrnhdms
      421 ltkasatfgi psttlwqrah rlgidtpkkd gptkswsdes lnnalealrt gtisankask
      481 aygipsstly kiarregirl aapfnasptt wspadldral eairsgqtsv qrastefgip
      541 tgtlygrckr egielsrsnp tpwsedamtd aleavrlghm sinqaaihyn lpysslygrf
      601 krgkyeepav gdlsqdgsnp hfhqsptqnh ssavpdqmpy qgs
//
LOCUS       XP_076546416             636 aa            linear   INV 03-SEP-2025
DEFINITION  pipsqueak isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546416
VERSION     XP_076546416.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690301.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="pipsqueak isoform X2"
                     /calculated_mol_wt=70366
     Region          44..128
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          396..431
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          450..488
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          505..550
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          557..599
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..636
                     /gene="Psq"
                     /coded_by="XM_076690301.1:118..2028"
                     /db_xref="GeneID:117606583"
ORIGIN      
        1 metdrywgvh ysggmagqhy clrwnnyqsn mtsvfhqllq teafvdvtla cneaslkahk
       61 vvlsacssyf qklllsnpck hptiimpqdv cfndlkfiie fvyrgeidvs qaelqsllkt
      121 adqlkikglc evpesrdgpp svslssppre pgtprinftk lkrhhprykr artsfeprat
      181 dsrhydryke eesnenynre nkenhrdwqa edeectegtt aavaletcqr nnnnnninns
      241 nnnnnngsns snsngtnnnn glghyghhpd pgdvdlppet qptppsatlv gttithlrdp
      301 dhhateiqnc dsvkikfetl htmdssdtid idshmsdras vssknaadnd nmmmitpell
      361 glmpsgssvh sdsgennsrg hsgqssshhh gskswtqedm daalealrnh dmsltkasat
      421 fgipsttlwq rahrlgidtp kkdgptksws deslnnalea lrtgtisank askaygipss
      481 tlykiarreg irlaapfnas pttwspadld raleairsgq tsvqrastef giptgtlygr
      541 ckregielsr snptpwseda mtdaleavrl ghmsinqaai hynlpyssly grfkrgkyee
      601 pavgdlsqdg snphfhqspt qnhssavpdq mpyqgs
//
LOCUS       XP_034185146             634 aa            linear   INV 03-SEP-2025
DEFINITION  pipsqueak isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034185146
VERSION     XP_034185146.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329255.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..634
                     /product="pipsqueak isoform X3"
                     /calculated_mol_wt=69949
     Region          35..119
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          394..>429
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          448..486
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          503..548
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          555..597
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..634
                     /gene="Psq"
                     /coded_by="XM_034329255.2:479..2383"
                     /db_xref="GeneID:117606583"
ORIGIN      
        1 mvsggmagqh yclrwnnyqs nmtsvfhqll qteafvdvtl acneaslkah kvvlsacssy
       61 fqklllsnpc khptiimpqd vcfndlkfii efvyrgeidv sqaelqsllk tadqlkikgl
      121 cevpesrdgp psvslssppr epgtprinft klkrhhpryk rartsfepra tdsrhydryk
      181 eeesnenynr enkenhrdwq aedeectegt taavaletcq rnnnnnninn snnnnnngsn
      241 ssnsngtnnn ngdmfchtgl ghyghhpdpg dvdlppetqp tppsatlvgt tithlrdpdh
      301 hateiqncds vkikfetlht mdssdtidid shmsdrasvs sknaadndnm mmitpellgl
      361 mpsgssvhsd sgennsrghs gqssshhhgs kswtqedmda alealrnhdm sltkasatfg
      421 ipsttlwqra hrlgidtpkk dgptkswsde slnnalealr tgtisankas kaygipsstl
      481 ykiarregir laapfnaspt twspadldra leairsgqts vqrastefgi ptgtlygrck
      541 regielsrsn ptpwsedamt daleavrlgh msinqaaihy nlpysslygr fkrgkyeepa
      601 vgdlsqdgsn phfhqsptqn hssavpdqmp yqgs
//
LOCUS       XP_034185155             629 aa            linear   INV 03-SEP-2025
DEFINITION  pipsqueak isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185155
VERSION     XP_034185155.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329264.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="pipsqueak isoform X4"
                     /calculated_mol_wt=69518
     Region          30..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          389..>424
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          443..481
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          498..543
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          550..592
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..629
                     /gene="Psq"
                     /coded_by="XM_034329264.2:130..2019"
                     /db_xref="GeneID:117606583"
ORIGIN      
        1 magqhyclrw nnyqsnmtsv fhqllqteaf vdvtlacnea slkahkvvls acssyfqkll
       61 lsnpckhpti impqdvcfnd lkfiiefvyr geidvsqael qsllktadql kikglcevpe
      121 srdgppsvsl sspprepgtp rinftklkrh hprykrarts fepratdsrh ydrykeeesn
      181 enynrenken hrdwqaedee ctegttaava letcqrnnnn nninnsnnnn nngsnssnsn
      241 gtnnnngdmf chtglghygh hpdpgdvdlp petqptppsa tlvgttithl rdpdhhatei
      301 qncdsvkikf etlhtmdssd tididshmsd rasvssknaa dndnmmmitp ellglmpsgs
      361 svhsdsgenn srghsgqsss hhhgskswtq edmdaaleal rnhdmsltka satfgipstt
      421 lwqrahrlgi dtpkkdgptk swsdeslnna lealrtgtis ankaskaygi psstlykiar
      481 regirlaapf naspttwspa dldraleair sgqtsvqras tefgiptgtl ygrckregie
      541 lsrsnptpws edamtdalea vrlghmsinq aaihynlpys slygrfkrgk yeepavgdls
      601 qdgsnphfhq sptqnhssav pdqmpyqgs
//
LOCUS       XP_034185176             629 aa            linear   INV 03-SEP-2025
DEFINITION  pipsqueak isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034185176
VERSION     XP_034185176.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329285.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="pipsqueak isoform X4"
                     /calculated_mol_wt=69518
     Region          30..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          389..>424
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          443..481
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          498..543
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     Region          550..592
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..629
                     /gene="Psq"
                     /coded_by="XM_034329285.2:114..2003"
                     /db_xref="GeneID:117606583"
ORIGIN      
        1 magqhyclrw nnyqsnmtsv fhqllqteaf vdvtlacnea slkahkvvls acssyfqkll
       61 lsnpckhpti impqdvcfnd lkfiiefvyr geidvsqael qsllktadql kikglcevpe
      121 srdgppsvsl sspprepgtp rinftklkrh hprykrarts fepratdsrh ydrykeeesn
      181 enynrenken hrdwqaedee ctegttaava letcqrnnnn nninnsnnnn nngsnssnsn
      241 gtnnnngdmf chtglghygh hpdpgdvdlp petqptppsa tlvgttithl rdpdhhatei
      301 qncdsvkikf etlhtmdssd tididshmsd rasvssknaa dndnmmmitp ellglmpsgs
      361 svhsdsgenn srghsgqsss hhhgskswtq edmdaaleal rnhdmsltka satfgipstt
      421 lwqrahrlgi dtpkkdgptk swsdeslnna lealrtgtis ankaskaygi psstlykiar
      481 regirlaapf naspttwspa dldraleair sgqtsvqras tefgiptgtl ygrckregie
      541 lsrsnptpws edamtdalea vrlghmsinq aaihynlpys slygrfkrgk yeepavgdls
      601 qdgsnphfhq sptqnhssav pdqmpyqgs
//
LOCUS       XP_034185196             580 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185196
VERSION     XP_034185196.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329305.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185196.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="uncharacterized protein LOC117606608 isoform X1"
                     /calculated_mol_wt=64806
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          390..423
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          435..455
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(440,442,444,446..447,450..451,454,469,471,475..476,
                     479..480,515,530,532,534,536..537,540..541)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          464..516
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          525..541
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          552..570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..580
                     /gene="LOC117606608"
                     /coded_by="XM_034329305.2:181..1923"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqasp rdyrvgeyrc nfcdcpyssq shlkrhltrg cfmdpsfpie
      421 qrhtlnrmes rnhvcpkcsq gyknkrtldt hlrtacgrep kfrcpycglr skhppniyth
      481 irrrhkdlss sseeskprvr ktykyklrds tssqkhdidt kpypcdscnk sflrfrtlks
      541 hkendcgitr tcptcmknfp ylasycrhik rcrassssss
//
LOCUS       XP_034185207             580 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185207
VERSION     XP_034185207.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329316.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185207.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="uncharacterized protein LOC117606608 isoform X1"
                     /calculated_mol_wt=64806
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          390..423
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          435..455
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(440,442,444,446..447,450..451,454,469,471,475..476,
                     479..480,515,530,532,534,536..537,540..541)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          464..516
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          525..541
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          552..570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..580
                     /gene="LOC117606608"
                     /coded_by="XM_034329316.2:294..2036"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqasp rdyrvgeyrc nfcdcpyssq shlkrhltrg cfmdpsfpie
      421 qrhtlnrmes rnhvcpkcsq gyknkrtldt hlrtacgrep kfrcpycglr skhppniyth
      481 irrrhkdlss sseeskprvr ktykyklrds tssqkhdidt kpypcdscnk sflrfrtlks
      541 hkendcgitr tcptcmknfp ylasycrhik rcrassssss
//
LOCUS       XP_034185213             580 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185213
VERSION     XP_034185213.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329322.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185213.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="uncharacterized protein LOC117606608 isoform X1"
                     /calculated_mol_wt=64806
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          390..423
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          435..455
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(440,442,444,446..447,450..451,454,469,471,475..476,
                     479..480,515,530,532,534,536..537,540..541)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          464..516
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          525..541
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          552..570
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..580
                     /gene="LOC117606608"
                     /coded_by="XM_034329322.2:182..1924"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqasp rdyrvgeyrc nfcdcpyssq shlkrhltrg cfmdpsfpie
      421 qrhtlnrmes rnhvcpkcsq gyknkrtldt hlrtacgrep kfrcpycglr skhppniyth
      481 irrrhkdlss sseeskprvr ktykyklrds tssqkhdidt kpypcdscnk sflrfrtlks
      541 hkendcgitr tcptcmknfp ylasycrhik rcrassssss
//
LOCUS       XP_034185219             579 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185219
VERSION     XP_034185219.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329328.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185219.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..579
                     /product="uncharacterized protein LOC117606608 isoform X2"
                     /calculated_mol_wt=64735
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          389..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          434..454
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(439,441,443,445..446,449..450,453,468,470,474..475,
                     478..479,514,529,531,533,535..536,539..540)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          463..515
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          524..540
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          551..569
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..579
                     /gene="LOC117606608"
                     /coded_by="XM_034329328.2:182..1921"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp gigawhvtgd rsnaggvvgs vagapgttde
      361 vflaaqeaaq ahrdsqaspr dyrvgeyrcn fcdcpyssqs hlkrhltrgc fmdpsfpieq
      421 rhtlnrmesr nhvcpkcsqg yknkrtldth lrtacgrepk frcpycglrs khppniythi
      481 rrrhkdlsss seeskprvrk tykyklrdst ssqkhdidtk pypcdscnks flrfrtlksh
      541 kendcgitrt cptcmknfpy lasycrhikr crassssss
//
LOCUS       XP_034185227             553 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185227
VERSION     XP_034185227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329336.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="uncharacterized protein LOC117606608 isoform X3"
                     /calculated_mol_wt=60949
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          437..457
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(442,444,446,448..449,452..453,456,475,477,481..482,
                     485..486,489,504,506,508,510..511,514..515)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          470..490
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          499..517
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..553
                     /gene="LOC117606608"
                     /coded_by="XM_034329336.2:181..1842"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqenk pdfraylkcl pplnfannaa tgggpfdppa igcspdprll
      421 fqgssawfpe tdkpymcssc gkgythiftl nrhrrtvcgk irntsgkwkc prctrsyvte
      481 gnlvrhvrfe cgvrrkfcci fcnrkftqrc slirhlrnfh nesfesasna vppeyppies
      541 cqaevkksed sii
//
LOCUS       XP_034185254             529 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185254
VERSION     XP_034185254.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329363.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185254.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..529
                     /product="uncharacterized protein LOC117606608 isoform X4"
                     /calculated_mol_wt=58878
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          460..476
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          489..510
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..529
                     /gene="LOC117606608"
                     /coded_by="XM_034329363.2:181..1770"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdsm ygqmhsslmr pqmvttrasv rvesyrpgsd avgkeakdes
      421 flyaepsgsy rrqpirsqts vhwpdnvtlq nsptrkkfyc prcnsgytrl sdmkthchfq
      481 cgkepryqcp yctkrakfss nmyvhvrrmh kdeklqiidl ykqlsriry
//
LOCUS       XP_034185262             524 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185262
VERSION     XP_034185262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..524
                     /product="uncharacterized protein LOC117606608 isoform X5"
                     /calculated_mol_wt=58224
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          425..458
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(430,432,434,436..437,440..441,457,479,481,485..486,
                     489..490,508,510,512,514..515,518..519,522)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          474..492
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          501..522
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          503..522
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..524
                     /gene="LOC117606608"
                     /coded_by="XM_034329371.2:181..1755"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgse ysssnwtvat lpttsrpvty trrlrsmtln ddqgknrwgp
      421 rkylcadcer tfalkasltr hrmfecgkqs laiektvhda vpaerkrkkk nylcpdcnkv
      481 yavftslwrh rhyecgvepk ftcpicrfrf sqksnldrhv rtkh
//
LOCUS       XP_034185271             514 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185271
VERSION     XP_034185271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329380.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="uncharacterized protein LOC117606608 isoform X6"
                     /calculated_mol_wt=56437
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          450..470
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          479..500
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..514
                     /gene="LOC117606608"
                     /coded_by="XM_034329380.2:181..1725"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqvpg fyaaapqldl nfvlqsckaf enegngrevs qtdgasvtrs
      421 lpnyyaqara rnayakggsf aecfrkpfgc pkcgrcftvk gnmtrhlkye cgqaprfqcp
      481 ycefrskqts nvmshirtrh tgqrvyvvhl kyen
//
LOCUS       XP_034185281             501 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185281
VERSION     XP_034185281.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329390.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="uncharacterized protein LOC117606608 isoform X7"
                     /calculated_mol_wt=55244
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          457..493
                     /region_name="Tnp_zf-ribbon_2"
                     /note="DDE_Tnp_1-like zinc-ribbon; pfam13842"
                     /db_xref="CDD:404687"
     CDS             1..501
                     /gene="LOC117606608"
                     /coded_by="XM_034329390.2:181..1686"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgpm dqsgsfnfqd qarsmeqssq hgfswigkkp sntsderlee
      421 kelvgeqats ntaprrller hfpetippte kkfnpvrqcv kcsrkkcvng krirketryy
      481 cpecnvglcv tpcfrlyhtv i
//
LOCUS       XP_034185315             494 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185315
VERSION     XP_034185315.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329424.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="uncharacterized protein LOC117606608 isoform X8"
                     /calculated_mol_wt=54519
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          411..431
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          438..463
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          440..461
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..494
                     /gene="LOC117606608"
                     /coded_by="XM_034329424.2:181..1665"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgfk liaasrsfsh paengriyrt swlpndtryp cgncssvfgq
      421 krsllthlry ecgqpprfkc pycdliskkt sniqkhirrk hegnavyvqd iyrspflavp
      481 ranlcdryyr salk
//
LOCUS       XP_034185322             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185322
VERSION     XP_034185322.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329431.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117606608 isoform X9"
                     /calculated_mol_wt=54178
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          422..442
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(427,429,431,433..434,437..438,441,456,458..460,
                     462..463,467)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          451..469
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..493
                     /gene="LOC117606608"
                     /coded_by="XM_034329431.2:181..1662"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdat tfgvfginfg iggvqvesvg kmyrqtsfgr lddnfktkkf
      421 pcpncacays qksslnrhlt yecgqeprfk cphceyrckk sanvyehvrr rhkncqvyai
      481 dvlkitgrpa nar
//
LOCUS       XP_076546417             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546417
VERSION     XP_076546417.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690302.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117606608 isoform
                     X10"
                     /calculated_mol_wt=54653
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          389..417
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(394,396,398,400..401,404..405,416,438,440,444..445,
                     448..449,466,468,470,472..473,476..477,481)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          433..452
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          461..482
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..493
                     /gene="LOC117606608"
                     /coded_by="XM_076690302.1:181..1662"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdwn dskanatncs qcgktflkqr dlifharhac dqirkirtvk
      421 ragaflyagn ldcdrcgktf krrkdlnyhv rhlcgirgiq cpycnkcyty tsnvkyhisr
      481 yhqgkevyyn klf
//
LOCUS       XP_034185330             490 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X11 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185330
VERSION     XP_034185330.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329439.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="uncharacterized protein LOC117606608 isoform
                     X11"
                     /calculated_mol_wt=53933
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          408..428
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          437..458
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..490
                     /gene="LOC117606608"
                     /coded_by="XM_034329439.2:181..1653"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdle hlggfkipcn afprqadiqe rnegrfpcpk ctscfnrknn
      421 lythlkfecg qlprfgcpyc hyaskkssni rahvrrkhre ykvsvidvsr epgygsfved
      481 ansietgqaf
//
LOCUS       XP_076546418             489 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X12 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546418
VERSION     XP_076546418.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690303.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="uncharacterized protein LOC117606608 isoform
                     X12"
                     /calculated_mol_wt=53245
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..489
                     /gene="LOC117606608"
                     /coded_by="XM_076690303.1:181..1650"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgrl rsafsslvrd awgrrrppag ihgpvqsvas ranassasgs
      421 nvcpkclvkf drvrdlrahm iracgfqakk mcpycdlagd naedihrhir rshpslrvav
      481 levynvdgs
//
LOCUS       XP_034185355             488 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X13 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185355
VERSION     XP_034185355.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329464.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..488
                     /product="uncharacterized protein LOC117606608 isoform
                     X13"
                     /calculated_mol_wt=53997
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          409..460
                     /region_name="Ribosomal_S27"
                     /note="Ribosomal protein S27a; cl00876"
                     /db_xref="CDD:469968"
     Region          427..447
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          456..477
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..488
                     /gene="LOC117606608"
                     /coded_by="XM_034329464.2:181..1647"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqvgh ayrvlyenyg ssypysrgep mkseeskfly ettvvrgrgn
      421 rrknyecpkc gngysvlksl rrhlryecgv apkfkcpyce irskqrahvn ehirrkhsgq
      481 rvyivdsp
//
LOCUS       XP_034185360             488 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X14 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185360
VERSION     XP_034185360.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329469.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..488
                     /product="uncharacterized protein LOC117606608 isoform
                     X14"
                     /calculated_mol_wt=53969
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          435..455
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          464..485
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..488
                     /gene="LOC117606608"
                     /coded_by="XM_034329469.2:181..1647"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgel iiwedyrgfd aatlaetgdf rsnscsyggr yfsdrkpvrv
      421 frnkcndpav grykcskcsk syrwkhhlve hvkafcgqkk aeccpycsyr snrkwnlksh
      481 mkrihadv
//
LOCUS       XP_034185371             487 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X15 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185371
VERSION     XP_034185371.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329480.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..487
                     /product="uncharacterized protein LOC117606608 isoform
                     X15"
                     /calculated_mol_wt=54085
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          417..437
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(422,424,426,428..429,432..433,436,451,453..455,
                     457..458,462)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          446..464
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..487
                     /gene="LOC117606608"
                     /coded_by="XM_034329480.2:181..1644"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgnf rrelkwndas fadspmeknl linkyrfypn erkqfeclnc
      421 gcrfsqktti trhlryfcgq gyryqcpycd mkascssniy rhvrsrhvgy kahaiklfvs
      481 istrrst
//
LOCUS       XP_034185377             485 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X16 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185377
VERSION     XP_034185377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="uncharacterized protein LOC117606608 isoform
                     X16"
                     /calculated_mol_wt=53630
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          425..>464
                     /region_name="cxxc_20_cxxc"
                     /note="cxxc_20_cxxc protein; TIGR04104"
                     /db_xref="CDD:274982"
     Region          425..445
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          454..473
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..485
                     /gene="LOC117606608"
                     /coded_by="XM_034329486.2:181..1638"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgrp lsgsydttsg vldmlranme ywdpndakkn eedhpwhsgk
      421 msyhcprcna gytykktlkt hmkydcgkep rfkcpycnkr dkcssniykh irvrhdgmpv
      481 lvhrn
//
LOCUS       XP_034185387             485 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X17 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185387
VERSION     XP_034185387.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="uncharacterized protein LOC117606608 isoform
                     X17"
                     /calculated_mol_wt=53517
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..485
                     /gene="LOC117606608"
                     /coded_by="XM_034329496.2:181..1638"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqaes kypggavfkh tcitcgkayk hkhhlkrhhd fecgidpkfk
      421 cafcphrtry kdslmkhila rhqylleqns qygpqqvldv hgeiegnsrm qgnmfasyhd
      481 fpqnl
//
LOCUS       XP_076546419             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X18 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546419
VERSION     XP_076546419.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690304.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC117606608 isoform
                     X18"
                     /calculated_mol_wt=53450
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          412..431
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(416,418,420,422..423,426..427,430,445,447..449,
                     451..452,456,461)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          440..462
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..484
                     /gene="LOC117606608"
                     /coded_by="XM_076690304.1:181..1635"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdfe krrwwlfggg etwrpeakyr kpqagvyvcp spncgksfnw
      421 kgnlnrhlry ecglqprfkc pyceyrckvk gdvskhitrr hhgsivyvvd lcnnpssipr
      481 svlg
//
LOCUS       XP_034185406             483 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X19 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185406
VERSION     XP_034185406.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329515.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="uncharacterized protein LOC117606608 isoform
                     X19"
                     /calculated_mol_wt=53494
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <382..468
                     /region_name="TackOD1"
                     /note="Thaumarchaeal output domain 1; pfam18551"
                     /db_xref="CDD:436569"
     Region          420..440
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          449..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..483
                     /gene="LOC117606608"
                     /coded_by="XM_034329515.2:181..1632"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgpm dqsgsfnfqd qarsmeqssq hgfswigkkp sntsderyac
      421 pkcersyrhl hhmqrhcrye cgrpprfqcp ycgmrskqsn nvykhirakh pgwkleivil
      481 hhd
//
LOCUS       XP_034185412             483 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X20 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185412
VERSION     XP_034185412.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329521.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="uncharacterized protein LOC117606608 isoform
                     X20"
                     /calculated_mol_wt=53160
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..483
                     /gene="LOC117606608"
                     /coded_by="XM_034329521.2:181..1632"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdff ppevftyass tgkrrrsnsk avvpvklivg ekqkfpcptc
      421 psvfshknnl yyhckfecgq lprfncpyct yrtkhvsnvr ahvrrkhpgn nvyaidvckm
      481 leg
//
LOCUS       XP_034185420             480 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X21 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185420
VERSION     XP_034185420.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329529.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..480
                     /product="uncharacterized protein LOC117606608 isoform
                     X21"
                     /calculated_mol_wt=53132
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          420..440
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          447..471
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          449..468
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..480
                     /gene="LOC117606608"
                     /coded_by="XM_034329529.2:181..1623"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdlp ipcitmpkny qrncnypmmp lmknlatlwm akrvefpyrc
      421 etcgkgyqhr atlvrhtrhe cgkepqfkcp ycthrtkqrg nlyqhirtnh pgknvfsssi
//
LOCUS       XP_034185426             478 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X22 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185426
VERSION     XP_034185426.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329535.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="uncharacterized protein LOC117606608 isoform
                     X22"
                     /calculated_mol_wt=53178
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          404..426
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..478
                     /gene="LOC117606608"
                     /coded_by="XM_034329535.2:181..1617"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgfs inswsleggs kqacdydawn rhpcpkcfrs ykhrshmirh
      421 ykyecgtsqr fecpyckhhl rqrthvwthi rtfhpnrely cvdiatnakl ihqehrtd
//
LOCUS       XP_034185436             476 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X23 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185436
VERSION     XP_034185436.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329545.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="uncharacterized protein LOC117606608 isoform
                     X23"
                     /calculated_mol_wt=52384
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          424..444
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          453..476
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          453..474
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..476
                     /gene="LOC117606608"
                     /coded_by="XM_034329545.2:181..1611"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqeat yyryhgrtag slyngaslps ivsgwktppk pgkgldrkpg
      421 cfrcprcnkg yrwlrnmrnh lkiqcgkdpn eccpycpyrt kyksslqrhi rgihfg
//
LOCUS       XP_076546420             476 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X24 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546420
VERSION     XP_076546420.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690305.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="uncharacterized protein LOC117606608 isoform
                     X24"
                     /calculated_mol_wt=53118
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          408..428
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          437..457
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..476
                     /gene="LOC117606608"
                     /coded_by="XM_076690305.1:181..1611"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgdv kevmrferfh grsgrefvii rrngmyecps chnlykwkks
      421 mlshlrnqcr qpprfecphc amknyqkahm irhlrvhhpq laqmfwdrkl ngffrl
//
LOCUS       XP_076546421             475 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X25 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546421
VERSION     XP_076546421.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690306.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="uncharacterized protein LOC117606608 isoform
                     X25"
                     /calculated_mol_wt=52355
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          410..430
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          437..458
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          439..460
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..475
                     /gene="LOC117606608"
                     /coded_by="XM_076690306.1:181..1608"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqddp fclerlmger gdvvsmsfaf qqvhnvrfpc pncssvfnrk
      421 nnlqkhlkye cgqlprfkcp yceyrskkts nirahvrtih fgcdvyvidl natcv
//
LOCUS       XP_076546422             473 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X26 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546422
VERSION     XP_076546422.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690307.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="uncharacterized protein LOC117606608 isoform
                     X26"
                     /calculated_mol_wt=52208
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          406..425
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(410,412,414,416..417,420..421,424,439,441..443,
                     445..446,450,453)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          432..457
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          434..453
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..473
                     /gene="LOC117606608"
                     /coded_by="XM_076690307.1:181..1602"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqglk gsllasaghr fangfvkphk facpnpncrs vfawkrnlts
      421 hlryqcgqkp rfkcpycdyl ckvkpdirkh irvkhknndv yvidifrswd llr
//
LOCUS       XP_034185443             472 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X27 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185443
VERSION     XP_034185443.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329552.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..472
                     /product="uncharacterized protein LOC117606608 isoform
                     X27"
                     /calculated_mol_wt=52362
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          401..421
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          430..448
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..472
                     /gene="LOC117606608"
                     /coded_by="XM_034329552.2:181..1599"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdld ridalmsvid rgskmanyyy cpkclrrytl ksnrnrhfrn
      421 ecgyeprfkc pycelkskqt sqiyhhirkw amrivyctri tgaairileg sr
//
LOCUS       XP_034185454             471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X28 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185454
VERSION     XP_034185454.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329563.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185454.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="uncharacterized protein LOC117606608 isoform
                     X28"
                     /calculated_mol_wt=51801
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          416..442
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          443..461
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..471
                     /gene="LOC117606608"
                     /coded_by="XM_034329563.2:181..1596"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdls ssseeskprv rktykyklrd stssqkhdid tkpypcdscn
      421 ksflrfrtlk shkendcgit rtcptcmknf pylasycrhi krcrasssss s
//
LOCUS       XP_034185463             471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X29 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185463
VERSION     XP_034185463.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329572.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="uncharacterized protein LOC117606608 isoform
                     X29"
                     /calculated_mol_wt=52388
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          404..424
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(409,411,413,415..416,419..420,423,438,440,444..445,
                     448..449,452,458,461,463..464,467..468)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          433..453
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..471
                     /gene="LOC117606608"
                     /coded_by="XM_034329572.2:181..1596"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgyt kskyyigqvp rdrrrsrgqg rfacdncdrr yhemknlrrh
      421 minecgkqpm hqcifcpyra tyrsylqvhm mkhtrqgftp rtanikqhrd s
//
LOCUS       XP_076546423             471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X30 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546423
VERSION     XP_076546423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690308.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="uncharacterized protein LOC117606608 isoform
                     X30"
                     /calculated_mol_wt=51748
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          404..424
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          407..>465
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          407..430
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Site            order(409,415,422,429,432,439)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          433..454
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          433..450
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..471
                     /gene="LOC117606608"
                     /coded_by="XM_076690308.1:181..1596"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgts sghvfegkvg rqksinatet pvrcprcgrs ykmkrnltth
      421 mkfecggqrn ftchicpaky tqniglrrhl lqrhniylpp kftvpkrifa l
//
LOCUS       XP_034185472             468 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X31 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185472
VERSION     XP_034185472.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329581.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..468
                     /product="uncharacterized protein LOC117606608 isoform
                     X31"
                     /calculated_mol_wt=51375
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          394..414
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(399,401,403,405..406,409..410,413,428,430..432,
                     434..435,439)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          423..440
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..468
                     /gene="LOC117606608"
                     /coded_by="XM_034329581.2:181..1587"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqetr tmgflncqrv typckncgkv ynyysslarh lkhecgvepk
      421 fhcplctyra khrsslnthl ngrhmkllnd fyampngnkl sssvdsgn
//
LOCUS       XP_034185481             467 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X32 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185481
VERSION     XP_034185481.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329590.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="uncharacterized protein LOC117606608 isoform
                     X32"
                     /calculated_mol_wt=51777
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..467
                     /gene="LOC117606608"
                     /coded_by="XM_034329590.2:181..1584"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdfr piflgkfhgv vrnsrhmlrl qtslnrrgfp cprcartfht
      421 sggmsrhyrl ecvdlprfkc phcdmrskyt qavyrhirak hrdmelr
//
LOCUS       XP_034185491             467 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X33 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185491
VERSION     XP_034185491.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329600.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="uncharacterized protein LOC117606608 isoform
                     X33"
                     /calculated_mol_wt=51524
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          406..426
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          435..455
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..467
                     /gene="LOC117606608"
                     /coded_by="XM_034329600.2:181..1584"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqall qmlplpwled kcperssrrs kirfpcprcr ksyttksavt
      421 ahfkydcgkp prfecpycgk lskkkfniqd hirhkhpskp vicntlf
//
LOCUS       XP_076546424             467 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X34 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546424
VERSION     XP_076546424.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690309.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..467
                     /product="uncharacterized protein LOC117606608 isoform
                     X34"
                     /calculated_mol_wt=51535
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          400..420
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(405,407,409,411..412,415..416,419,434,436..438,
                     440..441,445,449)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          427..447
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          429..450
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..467
                     /gene="LOC117606608"
                     /coded_by="XM_076690309.1:181..1584"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdtl afakqslfld nvsgrkkfpc pkcskvfvwk rtlrshtrne
      421 cgveprfrcp yceyrgkwkg nvlrhvksvh rdynieilnd itdelvg
//
LOCUS       XP_076546425             466 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X35 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546425
VERSION     XP_076546425.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690310.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="uncharacterized protein LOC117606608 isoform
                     X35"
                     /calculated_mol_wt=51871
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..466
                     /gene="LOC117606608"
                     /coded_by="XM_076690310.1:181..1581"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqaml wpykrlyvfe anrtarrndq qrerersfvc vdcgkayavh
      421 rslwrhrkfe cvnakpkftc evcpyksphr wcienhkkkh hqsvfn
//
LOCUS       XP_076546426             465 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X36 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546426
VERSION     XP_076546426.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690311.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..465
                     /product="uncharacterized protein LOC117606608 isoform
                     X36"
                     /calculated_mol_wt=51198
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          399..419
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          428..446
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..465
                     /gene="LOC117606608"
                     /coded_by="XM_076690311.1:181..1578"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqeli asgrcgrnrc frkvegftch qcgrgyqmrh nlvkhlrfec
      421 ggrkhfacsl cparytqngk lrqhmlnahn ifvpprrtrv kcays
//
LOCUS       XP_076546427             460 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X37 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546427
VERSION     XP_076546427.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690312.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..460
                     /product="uncharacterized protein LOC117606608 isoform
                     X37"
                     /calculated_mol_wt=51038
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          399..419
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          428..446
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..460
                     /gene="LOC117606608"
                     /coded_by="XM_076690312.1:181..1563"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgvc qnvtrrrrkm ysvnknyycp rcnhgftlkk nmtrhlrhec
      421 gmapkyqcpy cdkpskftqn iyahirkyhp gqvlcfrrly
//
LOCUS       XP_076546428             458 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X38 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546428
VERSION     XP_076546428.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690313.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..458
                     /product="uncharacterized protein LOC117606608 isoform
                     X38"
                     /calculated_mol_wt=50759
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <392..447
                     /region_name="PHA00733"
                     /note="hypothetical protein"
                     /db_xref="CDD:177301"
     Region          397..417
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          426..445
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..458
                     /gene="LOC117606608"
                     /coded_by="XM_076690313.1:181..1557"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqehp agswterkel mvqryvcprc cssyskkanm lthyryecgk
      421 eprfqcpycg krdrkssnty rhirtyhkds riqalkly
//
LOCUS       XP_076546429             457 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X39 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546429
VERSION     XP_076546429.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690314.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="uncharacterized protein LOC117606608 isoform
                     X39"
                     /calculated_mol_wt=50395
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          399..416
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          425..446
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..457
                     /gene="LOC117606608"
                     /coded_by="XM_076690314.1:181..1554"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdvr ilewsnartq aavvssyicp kcnagfrrmw dlirhkcgqi
      421 pryacpychk kdnsssnvyr hirrwhpklp vgvnkmf
//
LOCUS       XP_076546430             457 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X40 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546430
VERSION     XP_076546430.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690315.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="uncharacterized protein LOC117606608 isoform
                     X40"
                     /calculated_mol_wt=50064
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          402..422
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          429..453
                     /region_name="zf-H2C2_5"
                     /note="C2H2-type zinc-finger domain; pfam13909"
                     /db_xref="CDD:404746"
     Region          431..452
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..457
                     /gene="LOC117606608"
                     /coded_by="XM_076690315.1:181..1554"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgwg qselqlddsl tldqsgkpvf vcpkcgkgyt wkaslqrhls
      421 tgcglppmfr cklcdyrtsr kdilfrhirh vhsrvla
//
LOCUS       XP_034185516             455 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X41 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185516
VERSION     XP_034185516.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329625.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..455
                     /product="uncharacterized protein LOC117606608 isoform
                     X41"
                     /calculated_mol_wt=50550
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          390..410
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          420..441
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..455
                     /gene="LOC117606608"
                     /coded_by="XM_034329625.2:181..1548"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqgkq eskegpwfnc prcthsflsr dcmmrhfrsh cnlhetrfrc
      421 iycdygskys snvykhvrrv hrqmplfkah rwftc
//
LOCUS       XP_034185525             453 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X42 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185525
VERSION     XP_034185525.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329634.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="uncharacterized protein LOC117606608 isoform
                     X42"
                     /calculated_mol_wt=49422
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          394..423
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          424..447
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          426..447
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..453
                     /gene="LOC117606608"
                     /coded_by="XM_034329634.2:181..1542"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqasp mmegvsagel rfrcvmcgrg yrsrssltrh sryecgglgs
      421 rytftctlcg kyfcrpdhlk qhilnihggs vak
//
LOCUS       XP_034185534             452 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X43 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185534
VERSION     XP_034185534.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329643.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="uncharacterized protein LOC117606608 isoform
                     X43"
                     /calculated_mol_wt=49746
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..452
                     /gene="LOC117606608"
                     /coded_by="XM_034329643.2:181..1539"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdal krsllrheik kkegkvskvr rrcercyqen vkkfgsqvar
      421 nctvkvatfc encygkphlc lscfyaehfd mp
//
LOCUS       XP_034185550             449 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X44 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185550
VERSION     XP_034185550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329659.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="uncharacterized protein LOC117606608 isoform
                     X44"
                     /calculated_mol_wt=49528
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          398..427
                     /region_name="HTH_psq"
                     /note="helix-turn-helix, Psq domain; pfam05225"
                     /db_xref="CDD:283007"
     CDS             1..449
                     /gene="LOC117606608"
                     /coded_by="XM_034329659.2:181..1530"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp gggavarsrs rgnlggggga lwashfleck
      361 kgpnmrrprp dvdvgallks lankqgrkys ldsvkmslea ilqqgvsttq asemyginrs
      421 tlqfylkkln vvrrrwrqqp snlmqprqp
//
LOCUS       XP_034185554             448 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X45 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185554
VERSION     XP_034185554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329663.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..448
                     /product="uncharacterized protein LOC117606608 isoform
                     X45"
                     /calculated_mol_wt=49240
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          392..412
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(397,399,401,403..404,407..408,411,429,431..433,
                     435..436,440,444)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          424..445
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..448
                     /gene="LOC117606608"
                     /coded_by="XM_034329663.2:181..1527"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqdap lfpivrprlh vcprcgqmys wasnlrkhlk mgcgmvtfet
      421 hfscqycpyr srieasflkh llsvhnik
//
LOCUS       XP_034185563             447 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606608 isoform X46 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185563
VERSION     XP_034185563.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329672.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..447
                     /product="uncharacterized protein LOC117606608 isoform
                     X46"
                     /calculated_mol_wt=49162
     Region          31..114
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..447
                     /gene="LOC117606608"
                     /coded_by="XM_034329672.2:181..1524"
                     /db_xref="GeneID:117606608"
ORIGIN      
        1 meddqqfclr wnnhqstliq nfdtllesgt lvdctlaaeg kylkahkvvl sacspyfegl
       61 lsehydkhpv filkdvkfke lkammdymyr gevnisqdql aallkaaesl qikglseskt
      121 sgsnkmdtrq qkvvpqvtaa pcdipqgapg ltieknkvpt rqnlpqgsvg dlpedsaspq
      181 ippkglssre gsqsptsrkr krfrrrsige dnnsmenhea snsndmsqqv avpalgiapv
      241 aeetshvdpa dsigrsalmt qltrpademl qlplekpepn dsliepksey mddpeegved
      301 ltldddmndl nemepdnnra gpshdpsqhp agigawhvtg drsnaggvvg svagapgttd
      361 evflaaqeaa qahrdsqayr kmymgdqvlg ytctmcskfy kmwsnylkhk ceppqfkcpl
      421 cpfaafkafi lhahqaeqhf kvsspnt
//
LOCUS       XP_034185591             441 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial enolase superfamily member 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185591
VERSION     XP_034185591.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329700.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185591.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="mitochondrial enolase superfamily member 1"
                     /calculated_mol_wt=50157
     Region          4..416
                     /region_name="rTSbeta_L-fuconate_dehydratase"
                     /note="Human rTS beta is encoded by the rTS gene which,
                     through alternative RNA splicing, also encodes rTS alpha
                     whose mRNA is complementary to thymidylate synthase mRNA.
                     rTS beta expression is associated with the production of
                     small molecules that appear to...; cd03324"
                     /db_xref="CDD:239440"
     Site            order(187,217,219,247,271,273,302,325,352,383)
                     /site_type="active"
                     /note="putative active site pocket [active]"
                     /db_xref="CDD:239440"
     Site            order(247,273,302)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:239440"
     CDS             1..441
                     /gene="LOC117606791"
                     /coded_by="XM_034329700.2:62..1387"
                     /db_xref="GeneID:117606791"
ORIGIN      
        1 meviiteiev ndvrfptsll adgsdamhtd pdyscayvti ktnkgiegyg ltftlgrgte
       61 ivvqacksms ylvkgqkane iftnfgsfwr kltsesqlrw igpekgvthl ataaiinalw
      121 dlwariekkp vwklltdltp eqlvstvdfr yitdvitkee aiqllksske gkakreeilk
      181 kngypayttq vgwlgysdsk vkelcnkfln lgftsfktkv gqnlqddmrr cqmiretigy
      241 dnklmldanq vwdvnesiew mkqlikfkpt wieeptspdd vlghakiane lrphgvgvat
      301 gemcanrvmf kqflqmkaid ycqidsarig gineilavyl makkigvpvc phaggvglce
      361 mvqhlqmwdf iclsgttenr vieyidqqhe hfedpvvvqn acymppkqpg ysakfkddci
      421 rkysypdgeq wkymyekgif h
//
LOCUS       XP_034185604             264 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606798 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185604
VERSION     XP_034185604.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329713.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..264
                     /product="uncharacterized protein LOC117606798 isoform X1"
                     /calculated_mol_wt=29678
     CDS             1..264
                     /gene="LOC117606798"
                     /coded_by="XM_034329713.2:919..1713"
                     /db_xref="GeneID:117606798"
ORIGIN      
        1 mvsndrdsar vklasdkigg flesfqtriq ttwtmkwclv mlilagvtra dnsvestymn
       61 frcpplnsqe eidlnaimgk wyvvevlehk vdplkplsgs yvvnscpivk lraienaakf
      121 fsslrllwse emgnleytfr ipditrkpgf wisdsqqngt lvqkqysqft gnvyvmkava
      181 sdmvltfcag npdnqlysll larehilsks dkrgvhnllv rrglkivnir etcmnsaalr
      241 rasinligll glmgvlfnvl fgsw
//
LOCUS       XP_034185623             239 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606798 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185623
VERSION     XP_034185623.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329732.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="uncharacterized protein LOC117606798 isoform X2"
                     /calculated_mol_wt=26956
     CDS             1..239
                     /gene="LOC117606798"
                     /coded_by="XM_034329732.2:916..1635"
                     /db_xref="GeneID:117606798"
ORIGIN      
        1 mtriqttwtm kwclvmlila gvtradnsve stymnfrcpp lnsqeeidln aimgkwyvve
       61 vlehkvdplk plsgsyvvns cpivklraie naakffsslr llwseemgnl eytfripdit
      121 rkpgfwisds qqngtlvqkq ysqftgnvyv mkavasdmvl tfcagnpdnq lyslllareh
      181 ilsksdkrgv hnllvrrglk ivniretcmn saalrrasin ligllglmgv lfnvlfgsw
//
LOCUS       XP_034185641             230 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606798 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034185641
VERSION     XP_034185641.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329750.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="uncharacterized protein LOC117606798 isoform X3"
                     /calculated_mol_wt=25836
     CDS             1..230
                     /gene="LOC117606798"
                     /coded_by="XM_034329750.2:918..1610"
                     /db_xref="GeneID:117606798"
ORIGIN      
        1 mkwclvmlil agvtradnsv estymnfrcp plnsqeeidl naimgkwyvv evlehkvdpl
       61 kplsgsyvvn scpivklrai enaakffssl rllwseemgn leytfripdi trkpgfwisd
      121 sqqngtlvqk qysqftgnvy vmkavasdmv ltfcagnpdn qlyslllare hilsksdkrg
      181 vhnllvrrgl kivniretcm nsaalrrasi nligllglmg vlfnvlfgsw
//
LOCUS       XP_034185649             222 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar ATPase assembly protein VMA12 [Osmia lignaria lignaria].
ACCESSION   XP_034185649
VERSION     XP_034185649.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329758.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="vacuolar ATPase assembly protein VMA12"
                     /calculated_mol_wt=25408
     Region          80..207
                     /region_name="Vma12"
                     /note="Endoplasmic reticulum-based factor for assembly of
                     V-ATPase; pfam11712"
                     /db_xref="CDD:463329"
     CDS             1..222
                     /gene="LOC117606816"
                     /coded_by="XM_034329758.2:1..669"
                     /db_xref="GeneID:117606816"
ORIGIN      
        1 mvvesiedps ikikpnkkli eficknvkkt sntpssival kkstkdnqkd vllqlddirw
       61 lnkyleeyrt tkmesiylhe lldeadiklp tpklvprnpe leariqklka qqdareyqam
      121 tksidsnrrh fpedsisyqm kqinrqliav aqfvfsvvag fvfgfkgvdl iignldfgfr
      181 lllgiicali ialaeiyfla iklnedgyet vpqpmpkklh qe
//
LOCUS       XP_076546431             205 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305699 [Osmia lignaria lignaria].
ACCESSION   XP_076546431
VERSION     XP_076546431.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690316.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..205
                     /product="uncharacterized protein LOC143305699"
                     /calculated_mol_wt=23522
     CDS             1..205
                     /gene="LOC143305699"
                     /coded_by="XM_076690316.1:1..618"
                     /db_xref="GeneID:143305699"
ORIGIN      
        1 mlmpfksvph fteersfirt rtsdapvgfr scfspldlrt lpyphfltly ptslslslfs
       61 dsrlttetgr ssnhgrstfn pgreqgvkni kieadlenlc svvmeeakdf dyeeekplst
      121 rrrrsggvgv aatgglreie rhtcsrcsks yihawhlkrh tkfecgqepr vqcpyctarm
      181 kqrghvyrhi rqchrgqnvy vidln
//
LOCUS       XP_034185664             175 aa            linear   INV 03-SEP-2025
DEFINITION  chromatin accessibility complex 16kD protein-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034185664
VERSION     XP_034185664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="chromatin accessibility complex 16kD
                     protein-like isoform X1"
                     /calculated_mol_wt=19333
     Region          15..88
                     /region_name="HFD_CHRAC1-like"
                     /note="histone-fold domain found in chromatin
                     accessibility complex protein 1 (CHRAC-1) and similar
                     proteins; cd22924"
                     /db_xref="CDD:467049"
     Site            order(31..32,35..37,39..41,43..45,47..54,57,62..68,71,80,
                     83..84,87)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467049"
     CDS             1..175
                     /gene="LOC117606851"
                     /coded_by="XM_034329773.2:145..672"
                     /db_xref="GeneID:117606851"
ORIGIN      
        1 mtaqgspakm kelrlpmsrv ktimksspyv dtigqdglyl vtkatelfih ylteeahlqn
       61 nkgnsldykh laevvqtndt leflreimpr kitvrqfkem maaknsqsss esssdsdsds
      121 esdtnscsds dnrkadgnss nseqenetke ngncdsgsnk seaseksdse detkr
//
LOCUS       XP_076546432             141 aa            linear   INV 03-SEP-2025
DEFINITION  adenosine 5'-monophosphoramidase HINT3-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546432
VERSION     XP_076546432.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690317.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..141
                     /product="adenosine 5'-monophosphoramidase HINT3-like
                     isoform X2"
                     /calculated_mol_wt=16405
     Region          7..107
                     /region_name="HIT_like"
                     /note="HIT family: HIT (Histidine triad) proteins, named
                     for a motif related to the sequence HxHxH/Qxx (x, a
                     hydrophobic amino acid), are a superfamily of nucleotide
                     hydrolases and transferases, which act on the
                     alpha-phosphate of ribonucleotides. On the basis...;
                     cl00228"
                     /db_xref="CDD:469672"
     Site            order(32,34,42,44,89,98,102,104)
                     /site_type="active"
                     /note="nucleotide binding site/active site [active]"
                     /db_xref="CDD:238609"
     Site            order(100,102,104..106)
                     /site_type="active"
                     /note="HIT family signature motif [active]"
                     /db_xref="CDD:238609"
     Site            102
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238609"
     CDS             1..141
                     /gene="LOC117606851"
                     /coded_by="XM_076690317.1:166..591"
                     /db_xref="GeneID:117606851"
ORIGIN      
        1 mathvdncif ckiinnnaps ekvyedddmv cikdiypast hhylilprrh ianakelkpt
       61 daelydkmis tmhrmfemqg lnpaetrtgf hwppfntvhh lhlhiispis nmsilkrvmf
      121 kpdsywfvsv dyvksylqsn k
//
LOCUS       XP_076546433             175 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305700 [Osmia lignaria lignaria].
ACCESSION   XP_076546433
VERSION     XP_076546433.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690318.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="uncharacterized protein LOC143305700"
                     /calculated_mol_wt=19821
     Region          115..135
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          145..165
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..175
                     /gene="LOC143305700"
                     /coded_by="XM_076690318.1:1720..2247"
                     /db_xref="GeneID:143305700"
ORIGIN      
        1 mvalltllrs dykqqspqqp pqqqqqqqgm kktrsssglt attrstvvls grrvsasnag
       61 dnrdnnsyds gndnggrsst revvfpdsfe vlpqprdlsv iymgvpheqq rkfrcrfcgk
      121 gyrwkstmrr hemvecggkp pafqcpecpy karqrgnltv hykrhhqkid ydega
//
LOCUS       XP_076546434             174 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604380 [Osmia lignaria lignaria].
ACCESSION   XP_076546434
VERSION     XP_076546434.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690319.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..174
                     /product="uncharacterized protein LOC117604380"
                     /calculated_mol_wt=19893
     Region          <57..159
                     /region_name="Spt46"
                     /note="Spermatogenesis-associated protein 46; pfam17734"
                     /db_xref="CDD:375300"
     Region          122..142
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          149..171
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          151..171
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..174
                     /gene="LOC117604380"
                     /coded_by="XM_076690319.1:1014..1538"
                     /db_xref="GeneID:117604380"
ORIGIN      
        1 mnggnhrsek fqifqgselk elrndtdplv yflerkyspg aaiewyyqyq tlylrlmaes
       61 hqmatenpas edecsrkieg tgnddedsar seevlenlpn avsfigknat atsirdsssy
      121 scprcgnayt rphslsrhir fecgvepqfe cpvchkkskh khnlvlhmrt hqkh
//
LOCUS       XP_034185687             171 aa            linear   INV 03-SEP-2025
DEFINITION  biogenesis of lysosome-related organelles complex 1 subunit 5
            [Osmia lignaria lignaria].
ACCESSION   XP_034185687
VERSION     XP_034185687.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329796.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="biogenesis of lysosome-related organelles
                     complex 1 subunit 5"
                     /calculated_mol_wt=20325
     Region          24..167
                     /region_name="Muted"
                     /note="Organelle biogenesis, Muted-like protein;
                     pfam14942"
                     /db_xref="CDD:464390"
     CDS             1..171
                     /gene="Muted"
                     /coded_by="XM_034329796.2:154..669"
                     /db_xref="GeneID:117606826"
ORIGIN      
        1 mrenngnkfa ssapvlrrnm asvikdtgei wsrlfdhrpf vqgeitfflr efqekrgdte
       61 verlfkiley ttelkenqld rteqlgdchl pslkanvdva lsmcervfqr eqnfdtdtal
      121 qenrelrkle wekfvndmsd kcekvnqtfe ekekeikefy idlerklhit p
//
LOCUS       XP_076546435             165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606862 [Osmia lignaria lignaria].
ACCESSION   XP_076546435
VERSION     XP_076546435.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690320.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..165
                     /product="uncharacterized protein LOC117606862"
                     /calculated_mol_wt=18960
     Region          89..124
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          130..148
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..165
                     /gene="LOC117606862"
                     /coded_by="XM_076690320.1:918..1415"
                     /db_xref="GeneID:117606862"
ORIGIN      
        1 mhvsfgknvr ivktskmvvf ngervfagdk lleknaetle dddgedskic evfhgqlpmn
       61 yllgdssiln svetsllrkn vkshqqfrcd gcdrsytriy slkrhqmkcd aflenlqgea
      121 ewfhrqkfyc sqcgrgykrl dtlrrhqrla cekdnvstps dkats
//
LOCUS       XP_034185736             165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606862 [Osmia lignaria lignaria].
ACCESSION   XP_034185736
VERSION     XP_034185736.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329845.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034185736.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..165
                     /product="uncharacterized protein LOC117606862"
                     /calculated_mol_wt=18960
     Region          89..124
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          130..148
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..165
                     /gene="LOC117606862"
                     /coded_by="XM_034329845.2:918..1415"
                     /db_xref="GeneID:117606862"
ORIGIN      
        1 mhvsfgknvr ivktskmvvf ngervfagdk lleknaetle dddgedskic evfhgqlpmn
       61 yllgdssiln svetsllrkn vkshqqfrcd gcdrsytriy slkrhqmkcd aflenlqgea
      121 ewfhrqkfyc sqcgrgykrl dtlrrhqrla cekdnvstps dkats
//
LOCUS       XP_034185698             143 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606831 [Osmia lignaria lignaria].
ACCESSION   XP_034185698
VERSION     XP_034185698.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329807.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="uncharacterized protein LOC117606831"
                     /calculated_mol_wt=15522
     CDS             1..143
                     /gene="LOC117606831"
                     /coded_by="XM_034329807.2:98..529"
                     /db_xref="GeneID:117606831"
ORIGIN      
        1 mdrflhfvll favlpfairg aieeggceca vfqagssvsi ierplqynvt cnsegearcq
       61 llcvalaqsg rdkapqmice kmnahvenlq vavyskicna inwkftglkn sdlicchegk
      121 pipcsgsssi indwmdsitp lnp
//
LOCUS       XP_034185723             128 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117606856 [Osmia lignaria lignaria].
ACCESSION   XP_034185723
VERSION     XP_034185723.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034329832.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..128
                     /product="uncharacterized protein LOC117606856"
                     /calculated_mol_wt=15406
     Region          74..95
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(79,81,83,85..86,89..90,94,108,110..112,114..115,119,
                     122)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          103..123
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..128
                     /gene="LOC117606856"
                     /coded_by="XM_034329832.2:85..471"
                     /db_xref="GeneID:117606856"
ORIGIN      
        1 myrllvksaf tnrfsfstgy pmknvwsdhq sasmddmllk erhfeaiyfp ycpddpndkq
       61 qqqpqqiqpq lficslcgre ytwmyslrrh qlqcgnkear nkcqfcsrkf yrrdrlkehv
      121 lshhsnmi
//
LOCUS       XP_076546436             117 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117604641 [Osmia lignaria lignaria].
ACCESSION   XP_076546436
VERSION     XP_076546436.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690321.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..117
                     /product="uncharacterized protein LOC117604641"
                     /calculated_mol_wt=13511
     Region          46..66
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          75..92
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..117
                     /gene="LOC117604641"
                     /coded_by="XM_076690321.1:617..970"
                     /db_xref="GeneID:117604641"
ORIGIN      
        1 mllsqesqqp rensdlvwdh vamlgmpykp irswrnskda dpkyicnrcg ktykattsls
       61 rhkrlecgvv pcevcpicdr rfrhrfvlns hivgcrrklq nvsqkstdpl alpeere
//
LOCUS       XP_076546437             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305701 [Osmia lignaria lignaria].
ACCESSION   XP_076546437
VERSION     XP_076546437.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690322.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC143305701"
                     /calculated_mol_wt=13389
     Region          16..43
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(21,23,25,27..28,31..32,42,53,55,59..60,63..64,68,83,
                     85,87,89..90,93..94,97)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          42..>99
                     /region_name="ADNP_N"
                     /note="Activity-dependent neuroprotector homeobox protein
                     N-terminal; pfam19627"
                     /db_xref="CDD:466132"
     Region          48..69
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          78..97
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..116
                     /gene="LOC143305701"
                     /coded_by="XM_076690322.1:240..590"
                     /db_xref="GeneID:143305701"
ORIGIN      
        1 msgqtatsnc hkrhmcgfck kvfplknllr rhvqfgckmn prhsqfacsf cpykstykan
       61 merhvrnvhd tgvlkfrcdl cnfrsnysfc vrrhmktfhr aaddanatdp vntakk
//
LOCUS       XP_076546438             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305701 [Osmia lignaria lignaria].
ACCESSION   XP_076546438
VERSION     XP_076546438.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690323.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC143305701"
                     /calculated_mol_wt=13389
     Region          16..43
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(21,23,25,27..28,31..32,42,53,55,59..60,63..64,68,83,
                     85,87,89..90,93..94,97)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          42..>99
                     /region_name="ADNP_N"
                     /note="Activity-dependent neuroprotector homeobox protein
                     N-terminal; pfam19627"
                     /db_xref="CDD:466132"
     Region          48..69
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          78..97
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..116
                     /gene="LOC143305701"
                     /coded_by="XM_076690323.1:222..572"
                     /db_xref="GeneID:143305701"
ORIGIN      
        1 msgqtatsnc hkrhmcgfck kvfplknllr rhvqfgckmn prhsqfacsf cpykstykan
       61 merhvrnvhd tgvlkfrcdl cnfrsnysfc vrrhmktfhr aaddanatdp vntakk
//
LOCUS       XP_076546439             114 aa            linear   INV 03-SEP-2025
DEFINITION  longitudinals lacking protein-like [Osmia lignaria lignaria].
ACCESSION   XP_076546439
VERSION     XP_076546439.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690324.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..114
                     /product="longitudinals lacking protein-like"
                     /calculated_mol_wt=13215
     Region          <35..83
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Region          43..63
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          72..90
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..114
                     /gene="LOC143305702"
                     /coded_by="XM_076690324.1:1724..2068"
                     /db_xref="GeneID:143305702"
ORIGIN      
        1 meesadlpvq awkmqdqglp faelkipkac nyvgkkppgq fgcstcgrsy mrkdslqrhv
       61 hwecgkepqf qcpfcpqrck rkahwlrhir rlhidkigdm eayllaytpk leid
//
LOCUS       XP_076546440              89 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305703 [Osmia lignaria lignaria].
ACCESSION   XP_076546440
VERSION     XP_076546440.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690325.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="uncharacterized protein LOC143305703"
                     /calculated_mol_wt=10297
     Region          25..45
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          37..63
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          54..75
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..89
                     /gene="LOC143305703"
                     /coded_by="XM_076690325.1:158..427"
                     /db_xref="GeneID:143305703"
ORIGIN      
        1 mvqqfvtisa sktpltrflr trftcqqcgr gytmlcnlrr hmkwecggkr qfpcyycsys
       61 ftqktslqrh ltaihkidvv ngvepsiml
//
LOCUS       XP_034189690             687 aa            linear   INV 03-SEP-2025
DEFINITION  serine_rich_CAS and FAT-like_CAS_C domain-containing protein
            p130CAS isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189690
VERSION     XP_034189690.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333799.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..687
                     /product="serine_rich_CAS and FAT-like_CAS_C
                     domain-containing protein p130CAS isoform X1"
                     /calculated_mol_wt=74113
     Region          21..75
                     /region_name="SH3_CAS"
                     /note="Src homology 3 domain of CAS (Crk-Associated
                     Substrate) scaffolding proteins; cd11844"
                     /db_xref="CDD:212778"
     Site            order(25,27,30,34,55..56,69,71..72)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212778"
     Region          312..483
                     /region_name="Serine_rich"
                     /note="Serine rich protein interaction domain; pfam08824"
                     /db_xref="CDD:462610"
     Region          490..680
                     /region_name="CAS_C"
                     /note="Crk-Associated Substrate C-terminal domain;
                     pfam12026"
                     /db_xref="CDD:463437"
     CDS             1..687
                     /gene="p130CAS"
                     /coded_by="XM_034333799.2:90..2153"
                     /db_xref="GeneID:117608537"
ORIGIN      
        1 mlpqtpdiip ttlnqqkcvk aralydniae apdelafrkg dvltvleqnt aglegwwlca
       61 lrgrqgicpg nrlrllvgqy dtggclvgsr adlttsedgi qrhgkrrswh vqpnrvvtpq
      121 kcgdvylydl pasraspapp srhdsplnsn nehlhnsgry ttsnrssvdn ggdvsdcydv
      181 ppravpvips passpspaps cydiprppts ctpnsncsgg sgvtpldcyd vprslqpltp
      241 sssassltnd gslsgsnrss laapdydvpr srlpasslps rqntpvpktp tpppppqtqq
      301 iydvpvskel pleldsaleg lqrlqseasa aiarllgfvs pgwrtaqrld atlmdlrlaa
      361 lrlrtslhdl aefaegtlgn agkapdkgla sklrplvkal rdsdklvqea ateldamewd
      421 aeklcrgase tltptngpps tilstvqpdp ldqliacara ltedvrqvas fiqgnstllf
      481 krssiistgs snnsgagedy dyvnldsrev vakqreevra slpqelrsnf drvvsesdha
      541 tiqmppttpt pmdpndkqll afyaaqvith gnhlthaida fmqtvehnqp pkvflahgkf
      601 vvlsahrlvh igdtvhrnvt rndvrtrvlq canalneala qtvsktkqaa qffpsvsavq
      661 emvdsvvdvs hlakdlkvam ihaahqp
//
LOCUS       XP_034189699             686 aa            linear   INV 03-SEP-2025
DEFINITION  serine_rich_CAS and FAT-like_CAS_C domain-containing protein
            p130CAS isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034189699
VERSION     XP_034189699.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333808.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..686
                     /product="serine_rich_CAS and FAT-like_CAS_C
                     domain-containing protein p130CAS isoform X2"
                     /calculated_mol_wt=73979
     Region          20..74
                     /region_name="SH3_CAS"
                     /note="Src homology 3 domain of CAS (Crk-Associated
                     Substrate) scaffolding proteins; cd11844"
                     /db_xref="CDD:212778"
     Site            order(24,26,29,33,54..55,68,70..71)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212778"
     Region          311..482
                     /region_name="Serine_rich"
                     /note="Serine rich protein interaction domain; pfam08824"
                     /db_xref="CDD:462610"
     Region          489..679
                     /region_name="CAS_C"
                     /note="Crk-Associated Substrate C-terminal domain;
                     pfam12026"
                     /db_xref="CDD:463437"
     CDS             1..686
                     /gene="p130CAS"
                     /coded_by="XM_034333808.2:2740..4800"
                     /db_xref="GeneID:117608537"
ORIGIN      
        1 mlllirsagp sfdeqkcvka ralydniaea pdelafrkgd vltvleqnta glegwwlcal
       61 rgrqgicpgn rlrllvgqyd tggclvgsra dlttsedgiq rhgkrrswhv qpnrvvtpqk
      121 cgdvylydlp asraspapps rhdsplnsnn ehlhnsgryt tsnrssvdng gdvsdcydvp
      181 pravpvipsp asspspapsc ydiprpptsc tpnsncsggs gvtpldcydv prslqpltps
      241 ssassltndg slsgsnrssl aapdydvprs rlpasslpsr qntpvpktpt pppppqtqqi
      301 ydvpvskelp leldsalegl qrlqseasaa iarllgfvsp gwrtaqrlda tlmdlrlaal
      361 rlrtslhdla efaegtlgna gkapdkglas klrplvkalr dsdklvqeaa teldamewda
      421 eklcrgaset ltptngppst ilstvqpdpl dqliacaral tedvrqvasf iqgnstllfk
      481 rssiistgss nnsgagedyd yvnldsrevv akqreevras lpqelrsnfd rvvsesdhat
      541 iqmppttptp mdpndkqlla fyaaqvithg nhlthaidaf mqtvehnqpp kvflahgkfv
      601 vlsahrlvhi gdtvhrnvtr ndvrtrvlqc analnealaq tvsktkqaaq ffpsvsavqe
      661 mvdsvvdvsh lakdlkvami haahqp
//
LOCUS       XP_034189712             291 aa            linear   INV 03-SEP-2025
DEFINITION  yeti isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189712
VERSION     XP_034189712.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333821.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189712.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="yeti isoform X1"
                     /calculated_mol_wt=32737
     Region          216..283
                     /region_name="BCNT"
                     /note="Bucentaur or craniofacial development; pfam07572"
                     /db_xref="CDD:462213"
     CDS             1..291
                     /gene="Yeti"
                     /coded_by="XM_034333821.2:312..1187"
                     /db_xref="GeneID:117608547"
ORIGIN      
        1 mtdehqlpsd sdeddedyvp dgadsepvse vesegdvesg pedendenkr ettkkkgkkr
       61 gkvtksktkk rrtagknlkn eddekdeekd kteskekker teeeekkrad slwadfmkdt
      121 avgskskpqd tinkpkeksp plekpkveek vkitkvfefa geevkvekev pidsaearlt
      181 lssaenseks gntaspagkg sgrgkgfkra glggissvlt qigkkakist lekskldwdn
      241 ykkeenleee itthnkgkdg ylerqdflqr adlrqfeiek qlrntnrrss r
//
LOCUS       XP_034189722             291 aa            linear   INV 03-SEP-2025
DEFINITION  yeti isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189722
VERSION     XP_034189722.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189722.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="yeti isoform X1"
                     /calculated_mol_wt=32737
     Region          216..283
                     /region_name="BCNT"
                     /note="Bucentaur or craniofacial development; pfam07572"
                     /db_xref="CDD:462213"
     CDS             1..291
                     /gene="Yeti"
                     /coded_by="XM_034333831.2:316..1191"
                     /db_xref="GeneID:117608547"
ORIGIN      
        1 mtdehqlpsd sdeddedyvp dgadsepvse vesegdvesg pedendenkr ettkkkgkkr
       61 gkvtksktkk rrtagknlkn eddekdeekd kteskekker teeeekkrad slwadfmkdt
      121 avgskskpqd tinkpkeksp plekpkveek vkitkvfefa geevkvekev pidsaearlt
      181 lssaenseks gntaspagkg sgrgkgfkra glggissvlt qigkkakist lekskldwdn
      241 ykkeenleee itthnkgkdg ylerqdflqr adlrqfeiek qlrntnrrss r
//
LOCUS       XP_034189732             291 aa            linear   INV 03-SEP-2025
DEFINITION  yeti isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034189732
VERSION     XP_034189732.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333841.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189732.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="yeti isoform X1"
                     /calculated_mol_wt=32737
     Region          216..283
                     /region_name="BCNT"
                     /note="Bucentaur or craniofacial development; pfam07572"
                     /db_xref="CDD:462213"
     CDS             1..291
                     /gene="Yeti"
                     /coded_by="XM_034333841.2:170..1045"
                     /db_xref="GeneID:117608547"
ORIGIN      
        1 mtdehqlpsd sdeddedyvp dgadsepvse vesegdvesg pedendenkr ettkkkgkkr
       61 gkvtksktkk rrtagknlkn eddekdeekd kteskekker teeeekkrad slwadfmkdt
      121 avgskskpqd tinkpkeksp plekpkveek vkitkvfefa geevkvekev pidsaearlt
      181 lssaenseks gntaspagkg sgrgkgfkra glggissvlt qigkkakist lekskldwdn
      241 ykkeenleee itthnkgkdg ylerqdflqr adlrqfeiek qlrntnrrss r
//
LOCUS       XP_034189749             290 aa            linear   INV 03-SEP-2025
DEFINITION  yeti isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034189749
VERSION     XP_034189749.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333858.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189749.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..290
                     /product="yeti isoform X2"
                     /calculated_mol_wt=32666
     Region          215..282
                     /region_name="BCNT"
                     /note="Bucentaur or craniofacial development; pfam07572"
                     /db_xref="CDD:462213"
     CDS             1..290
                     /gene="Yeti"
                     /coded_by="XM_034333858.2:312..1184"
                     /db_xref="GeneID:117608547"
ORIGIN      
        1 mtdehqlpsd sdeddedyvp dgadsepvse vesegdvesg pedendenkr ettkkkgkkr
       61 gkvtksktkk rrtagknlkn eddekdeekd kteskekker teeeekkrad slwadfmkdt
      121 vgskskpqdt inkpkekspp lekpkveekv kitkvfefag eevkvekevp idsaearltl
      181 ssaenseksg ntaspagkgs grgkgfkrag lggissvltq igkkakistl ekskldwdny
      241 kkeenleeei tthnkgkdgy lerqdflqra dlrqfeiekq lrntnrrssr
//
LOCUS       XP_034189762             280 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608563 [Osmia lignaria lignaria].
ACCESSION   XP_034189762
VERSION     XP_034189762.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333871.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="uncharacterized protein LOC117608563"
                     /calculated_mol_wt=32693
     CDS             1..280
                     /gene="LOC117608563"
                     /coded_by="XM_034333871.2:301..1143"
                     /db_xref="GeneID:117608563"
ORIGIN      
        1 mrknccdpvc sanrqfacts rsksrskkrk lacyeacptt srtstkprkr cakskkrske
       61 qkvkicknkk craarwdves stsihvpeki cvrrtesmrs crscqdsrrp kritctchpr
      121 rsiplerkke nkccqfkkcr rqtrqlmplc icvrtsssen frsiqsspcp vfskygeccc
      181 ishkkkrsck etpvaprrvs ehrkcckcqy nptslktqqn vflrglsffg rkiqklrhpf
      241 snnprpnrlr scpinkccsr salkrrndnf perrkvmfck
//
LOCUS       XP_034189772             280 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608563 [Osmia lignaria lignaria].
ACCESSION   XP_034189772
VERSION     XP_034189772.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333881.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="uncharacterized protein LOC117608563"
                     /calculated_mol_wt=32693
     CDS             1..280
                     /gene="LOC117608563"
                     /coded_by="XM_034333881.2:297..1139"
                     /db_xref="GeneID:117608563"
ORIGIN      
        1 mrknccdpvc sanrqfacts rsksrskkrk lacyeacptt srtstkprkr cakskkrske
       61 qkvkicknkk craarwdves stsihvpeki cvrrtesmrs crscqdsrrp kritctchpr
      121 rsiplerkke nkccqfkkcr rqtrqlmplc icvrtsssen frsiqsspcp vfskygeccc
      181 ishkkkrsck etpvaprrvs ehrkcckcqy nptslktqqn vflrglsffg rkiqklrhpf
      241 snnprpnrlr scpinkccsr salkrrndnf perrkvmfck
//
LOCUS       XP_034189787             280 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608563 [Osmia lignaria lignaria].
ACCESSION   XP_034189787
VERSION     XP_034189787.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333896.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="uncharacterized protein LOC117608563"
                     /calculated_mol_wt=32693
     CDS             1..280
                     /gene="LOC117608563"
                     /coded_by="XM_034333896.2:301..1143"
                     /db_xref="GeneID:117608563"
ORIGIN      
        1 mrknccdpvc sanrqfacts rsksrskkrk lacyeacptt srtstkprkr cakskkrske
       61 qkvkicknkk craarwdves stsihvpeki cvrrtesmrs crscqdsrrp kritctchpr
      121 rsiplerkke nkccqfkkcr rqtrqlmplc icvrtsssen frsiqsspcp vfskygeccc
      181 ishkkkrsck etpvaprrvs ehrkcckcqy nptslktqqn vflrglsffg rkiqklrhpf
      241 snnprpnrlr scpinkccsr salkrrndnf perrkvmfck
//
LOCUS       XP_034189802             250 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608571 [Osmia lignaria lignaria].
ACCESSION   XP_034189802
VERSION     XP_034189802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333911.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..250
                     /product="uncharacterized protein LOC117608571"
                     /calculated_mol_wt=28748
     Region          21..152
                     /region_name="TSSC4"
                     /note="Tumour suppressing sub-chromosomal transferable
                     candidate 4; pfam15264"
                     /db_xref="CDD:434584"
     CDS             1..250
                     /gene="LOC117608571"
                     /coded_by="XM_034333911.2:352..1104"
                     /db_xref="GeneID:117608571"
ORIGIN      
        1 mhtsgdfvih ggnsafanrq kllfdklsna eqecnknkst iestemdidv deqkkslvpr
       61 hgqkrksetk rfrgkesifk rpegpapras nrnvpdyhkn phkwtkysld dvsnddmteq
      121 sntraalsfl kelktrrsie indvemdvdd pnlidknqsk ivfksrknkt tsniqfkkpe
      181 neientddnd avviddedkp tfksskvimp eyivgqkqkk kskkekpakk idqskqlkld
      241 hleeadeeed
//
LOCUS       XP_034189815             184 aa            linear   INV 03-SEP-2025
DEFINITION  GNAT family N-acetyltransferase vnc [Osmia lignaria lignaria].
ACCESSION   XP_034189815
VERSION     XP_034189815.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333924.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..184
                     /product="GNAT family N-acetyltransferase vnc"
                     /calculated_mol_wt=20939
     Region          56..154
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..184
                     /gene="vnc"
                     /coded_by="XM_034333924.2:663..1217"
                     /db_xref="GeneID:117608576"
ORIGIN      
        1 msinircatt ddllniqhcn lqclpenyqm kyylyhalsw pqlsyvaede kgrivgyvla
       61 kmeedcednp hghitslavk rshrrlgiaq klmnqasram vecfgakyvs lhvrrsnraa
      121 lnlytsslqf evsevepkyy adgedayamk rdlssfhmek naakekaikd gnththtgrc
      181 cdrs
//
LOCUS       XP_034189833             184 aa            linear   INV 03-SEP-2025
DEFINITION  GNAT family N-acetyltransferase vnc [Osmia lignaria lignaria].
ACCESSION   XP_034189833
VERSION     XP_034189833.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333942.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..184
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..184
                     /product="GNAT family N-acetyltransferase vnc"
                     /calculated_mol_wt=20939
     Region          56..154
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..184
                     /gene="vnc"
                     /coded_by="XM_034333942.2:740..1294"
                     /db_xref="GeneID:117608576"
ORIGIN      
        1 msinircatt ddllniqhcn lqclpenyqm kyylyhalsw pqlsyvaede kgrivgyvla
       61 kmeedcednp hghitslavk rshrrlgiaq klmnqasram vecfgakyvs lhvrrsnraa
      121 lnlytsslqf evsevepkyy adgedayamk rdlssfhmek naakekaikd gnththtgrc
      181 cdrs
//
LOCUS       XP_034189848             867 aa            linear   INV 03-SEP-2025
DEFINITION  puromycin-sensitive aminopeptidase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189848
VERSION     XP_034189848.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333957.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..867
                     /product="puromycin-sensitive aminopeptidase isoform X1"
                     /calculated_mol_wt=98155
     Region          20..455
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(137,139,272..275,308..309,312,331,335,389,394)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          533..847
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..867
                     /gene="Psa"
                     /coded_by="XM_034333957.2:169..2772"
                     /db_xref="GeneID:117608590"
ORIGIN      
        1 msstekkpfr rlptdvqpyh yhivlspnlk tfvfdgkedv hidvkqstdt ivlnsldidi
       61 knaafnakdg kvietskidl capeetatlv fneklpsgks gylslefvge indkmkgfyr
      121 skyigtngtv ehaavtqfep tdarrcfpcw depahkatfd itlnvpsglt alsnmpiksk
      181 vtnestetlv fertpimsty lvavvvgefd yiedkssdgv lvrvytpklk keqgqfalev
      241 atkvlpyykt yfgiayplpk idliaiadfs sgamenwglv tyretcllvd pqntsavrkq
      301 wialvvahel ahqwfgnlvt mewwthlwln egyasfvefl cvahlfpeyd iwtqfvtdty
      361 iraleldalk nshpievpvg hpseideifd disynkgacv irmlhsyigd ddfrkgmnly
      421 lkkhsygnae tgdlwdalee asnknvrsvm stwteqqgfp vvrvqhrqeg tdrvlslsqe
      481 rfladgstdt gtslwiipis istakdpeec vlkalldekt kelrvkdvpe nhwvkinpgt
      541 igfyrthysp ealslllpav kdhalppldr lgllddlfam vqaghastve vlqlmqafqk
      601 ednytvwssi vnslgkigvl vshldfedsf kaygrnlmrd itnklgwdpk pteshldtll
      661 rslvlgrmaa lndedtikea kkrfelhvsg ttllaadlrs pvyravlsvg dadtyetmlr
      721 lyreadlhee kdrilralga ikdetllakv lnfamsdevr aqdtvfaims vamtykgrvm
      781 awdffkenwk tlldrygggf lisrlvkftt enfvteerak dveeffkshp tpgtertvqq
      841 siesirlnas wlardkdsik eyltthv
//
LOCUS       XP_034189854             866 aa            linear   INV 03-SEP-2025
DEFINITION  puromycin-sensitive aminopeptidase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189854
VERSION     XP_034189854.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333963.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..866
                     /product="puromycin-sensitive aminopeptidase isoform X2"
                     /calculated_mol_wt=98229
     Region          20..454
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(134,136,271..274,307..308,311,330,334,388,393)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Region          532..846
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     CDS             1..866
                     /gene="Psa"
                     /coded_by="XM_034333963.2:172..2772"
                     /db_xref="GeneID:117608590"
ORIGIN      
        1 msstekkpfr rlptdvqpyh yhivlspnlk tfvfdgkedv hidvkqstdt ivlnsldidi
       61 knaafnakdg kvietskidl capeetatlv fneklpsgks gylslefvge indkmkgfyr
      121 skyigenkfa avtflcptsa rklfpcwdep ilkatftiql efpcnsqlig lsnmpikskv
      181 tnestetlvf ertpimstyl vavvvgefdy iedkssdgvl vrvytpklkk eqgqfaleva
      241 tkvlpyykty fgiayplpki dliaiadfss gamenwglvt yretcllvdp qntsavrkqw
      301 ialvvahela hqwfgnlvtm ewwthlwlne gyasfveflc vahlfpeydi wtqfvtdtyi
      361 raleldalkn shpievpvgh pseideifdd isynkgacvi rmlhsyigdd dfrkgmnlyl
      421 kkhsygnaet gdlwdaleea snknvrsvms twteqqgfpv vrvqhrqegt drvlslsqer
      481 fladgstdtg tslwiipisi stakdpeecv lkalldektk elrvkdvpen hwvkinpgti
      541 gfyrthyspe alslllpavk dhalppldrl gllddlfamv qaghastvev lqlmqafqke
      601 dnytvwssiv nslgkigvlv shldfedsfk aygrnlmrdi tnklgwdpkp teshldtllr
      661 slvlgrmaal ndedtikeak krfelhvsgt tllaadlrsp vyravlsvgd adtyetmlrl
      721 yreadlheek drilralgai kdetllakvl nfamsdevra qdtvfaimsv amtykgrvma
      781 wdffkenwkt lldrygggfl isrlvkftte nfvteerakd veeffkshpt pgtertvqqs
      841 iesirlnasw lardkdsike yltthv
//
LOCUS       XP_034189865             511 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189865
VERSION     XP_034189865.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333974.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="cyclin dependent kinase Eip63E isoform X1"
                     /calculated_mol_wt=58103
     Region          181..469
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(188..191,196,209,211,227,241,257..260,263,265..266,
                     306,308,310..311,313,324,327,339,341..344,346,384..385)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(188..191,196,209,211,241,257..260,263,266,310..311,
                     313,324)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(219..221,223,226..227,229..230,233..234,246,248,253,
                     295,298..301,329,331..334,338..339,455,459..461)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(227,265,306,308,327,339,341..344,346,384..385)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            323..346
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..511
                     /gene="Eip63E"
                     /coded_by="XM_034333974.2:520..2055"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mrewkkvllc tgkhaehpet rcgvsshsrn yqrsvtmrek kggalsrvqk lkkrlshsfg
       61 rlsiskeead daanrgqlpy ngyseefldr lepngnipad kdrryewtgv gdhervhrql
      121 svssdsklld edireearvi lrprrpprpk sevflgpdpp prrtkrfsaf ggdspfgkse
      181 ayikleqlge gsyatvfkgy shltnqvval keirlqeeeg apftaireas llkelkhsni
      241 vtlhdiihtr etltfvfeyv htdlsqymer ygsgnggldp rnvklflfql lrglaychrr
      301 rvlhrdvkpq nlliseigel kladfglara ksvpshtysh evvtlwyrpp dvllgsteys
      361 tsldmwgvgc ifmemltgep tfpgvrctyd qldkifkvlg tpteetwpgv thlpgykphr
      421 lgfypprklg lsfprlydia egdsmassll qlnpdqriga eealrhpyfa slprklyelp
      481 devsifsveg chlytntrhg cadsrhtalk t
//
LOCUS       XP_034189875             511 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189875
VERSION     XP_034189875.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333984.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="cyclin dependent kinase Eip63E isoform X1"
                     /calculated_mol_wt=58103
     Region          181..469
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(188..191,196,209,211,227,241,257..260,263,265..266,
                     306,308,310..311,313,324,327,339,341..344,346,384..385)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(188..191,196,209,211,241,257..260,263,266,310..311,
                     313,324)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(219..221,223,226..227,229..230,233..234,246,248,253,
                     295,298..301,329,331..334,338..339,455,459..461)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(227,265,306,308,327,339,341..344,346,384..385)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            323..346
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..511
                     /gene="Eip63E"
                     /coded_by="XM_034333984.2:521..2056"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mrewkkvllc tgkhaehpet rcgvsshsrn yqrsvtmrek kggalsrvqk lkkrlshsfg
       61 rlsiskeead daanrgqlpy ngyseefldr lepngnipad kdrryewtgv gdhervhrql
      121 svssdsklld edireearvi lrprrpprpk sevflgpdpp prrtkrfsaf ggdspfgkse
      181 ayikleqlge gsyatvfkgy shltnqvval keirlqeeeg apftaireas llkelkhsni
      241 vtlhdiihtr etltfvfeyv htdlsqymer ygsgnggldp rnvklflfql lrglaychrr
      301 rvlhrdvkpq nlliseigel kladfglara ksvpshtysh evvtlwyrpp dvllgsteys
      361 tsldmwgvgc ifmemltgep tfpgvrctyd qldkifkvlg tpteetwpgv thlpgykphr
      421 lgfypprklg lsfprlydia egdsmassll qlnpdqriga eealrhpyfa slprklyelp
      481 devsifsveg chlytntrhg cadsrhtalk t
//
LOCUS       XP_034189885             494 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189885
VERSION     XP_034189885.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="cyclin dependent kinase Eip63E isoform X2"
                     /calculated_mol_wt=55928
     Region          164..452
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(171..174,179,192,194,210,224,240..243,246,248..249,
                     289,291,293..294,296,307,310,322,324..327,329,367..368)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(171..174,179,192,194,224,240..243,246,249,293..294,
                     296,307)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(202..204,206,209..210,212..213,216..217,229,231,236,
                     278,281..284,312,314..317,321..322,438,442..444)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(210,248,289,291,310,322,324..327,329,367..368)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            306..329
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..494
                     /gene="Eip63E"
                     /coded_by="XM_034333994.2:508..1992"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mycqdkssta skskegsvtm rekkggalsr vqklkkrlsh sfgrlsiske eaddaanrgq
       61 lpyngyseef ldrlepngni padkdrryew tgvgdhervh rqlsvssdsk lldedireea
      121 rvilrprrpp rpksevflgp dppprrtkrf safggdspfg kseayikleq lgegsyatvf
      181 kgyshltnqv valkeirlqe eegapftair easllkelkh snivtlhdii htretltfvf
      241 eyvhtdlsqy merygsgngg ldprnvklfl fqllrglayc hrrrvlhrdv kpqnllisei
      301 gelkladfgl araksvpsht yshevvtlwy rppdvllgst eystsldmwg vgcifmemlt
      361 geptfpgvrc tydqldkifk vlgtpteetw pgvthlpgyk phrlgfyppr klglsfprly
      421 diaegdsmas sllqlnpdqr igaeealrhp yfaslprkly elpdevsifs vegchlytnt
      481 rhgcadsrht alkt
//
LOCUS       XP_034189895             481 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189895
VERSION     XP_034189895.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334004.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="cyclin dependent kinase Eip63E isoform X3"
                     /calculated_mol_wt=54669
     Region          151..439
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(158..161,166,179,181,197,211,227..230,233,235..236,
                     276,278,280..281,283,294,297,309,311..314,316,354..355)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(158..161,166,179,181,211,227..230,233,236,280..281,
                     283,294)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(189..191,193,196..197,199..200,203..204,216,218,223,
                     265,268..271,299,301..304,308..309,425,429..431)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(197,235,276,278,297,309,311..314,316,354..355)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            293..316
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..481
                     /gene="Eip63E"
                     /coded_by="XM_034334004.2:582..2027"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mwrsvtmrek kggalsrvqk lkkrlshsfg rlsiskeead daanrgqlpy ngyseefldr
       61 lepngnipad kdrryewtgv gdhervhrql svssdsklld edireearvi lrprrpprpk
      121 sevflgpdpp prrtkrfsaf ggdspfgkse ayikleqlge gsyatvfkgy shltnqvval
      181 keirlqeeeg apftaireas llkelkhsni vtlhdiihtr etltfvfeyv htdlsqymer
      241 ygsgnggldp rnvklflfql lrglaychrr rvlhrdvkpq nlliseigel kladfglara
      301 ksvpshtysh evvtlwyrpp dvllgsteys tsldmwgvgc ifmemltgep tfpgvrctyd
      361 qldkifkvlg tpteetwpgv thlpgykphr lgfypprklg lsfprlydia egdsmassll
      421 qlnpdqriga eealrhpyfa slprklyelp devsifsveg chlytntrhg cadsrhtalk
      481 t
//
LOCUS       XP_034189903             475 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189903
VERSION     XP_034189903.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334012.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="cyclin dependent kinase Eip63E isoform X4"
                     /calculated_mol_wt=53908
     Region          145..433
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(152..155,160,173,175,191,205,221..224,227,229..230,
                     270,272,274..275,277,288,291,303,305..308,310,348..349)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(152..155,160,173,175,205,221..224,227,230,274..275,
                     277,288)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(183..185,187,190..191,193..194,197..198,210,212,217,
                     259,262..265,293,295..298,302..303,419,423..425)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(191,229,270,272,291,303,305..308,310,348..349)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            287..310
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..475
                     /gene="Eip63E"
                     /coded_by="XM_034334012.2:594..2021"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mrekkggals rvqklkkrls hsfgrlsisk eeaddaanrg qlpyngysee fldrlepngn
       61 ipadkdrrye wtgvgdherv hrqlsvssds klldediree arvilrprrp prpksevflg
      121 pdppprrtkr fsafggdspf gkseayikle qlgegsyatv fkgyshltnq vvalkeirlq
      181 eeegapftai reasllkelk hsnivtlhdi ihtretltfv feyvhtdlsq ymerygsgng
      241 gldprnvklf lfqllrglay chrrrvlhrd vkpqnllise igelkladfg laraksvpsh
      301 tyshevvtlw yrppdvllgs teystsldmw gvgcifmeml tgeptfpgvr ctydqldkif
      361 kvlgtpteet wpgvthlpgy kphrlgfypp rklglsfprl ydiaegdsma ssllqlnpdq
      421 rigaeealrh pyfaslprkl yelpdevsif svegchlytn trhgcadsrh talkt
//
LOCUS       XP_034189911             475 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189911
VERSION     XP_034189911.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="cyclin dependent kinase Eip63E isoform X4"
                     /calculated_mol_wt=53908
     Region          145..433
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(152..155,160,173,175,191,205,221..224,227,229..230,
                     270,272,274..275,277,288,291,303,305..308,310,348..349)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(152..155,160,173,175,205,221..224,227,230,274..275,
                     277,288)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(183..185,187,190..191,193..194,197..198,210,212,217,
                     259,262..265,293,295..298,302..303,419,423..425)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(191,229,270,272,291,303,305..308,310,348..349)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            287..310
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..475
                     /gene="Eip63E"
                     /coded_by="XM_034334020.2:269..1696"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mrekkggals rvqklkkrls hsfgrlsisk eeaddaanrg qlpyngysee fldrlepngn
       61 ipadkdrrye wtgvgdherv hrqlsvssds klldediree arvilrprrp prpksevflg
      121 pdppprrtkr fsafggdspf gkseayikle qlgegsyatv fkgyshltnq vvalkeirlq
      181 eeegapftai reasllkelk hsnivtlhdi ihtretltfv feyvhtdlsq ymerygsgng
      241 gldprnvklf lfqllrglay chrrrvlhrd vkpqnllise igelkladfg laraksvpsh
      301 tyshevvtlw yrppdvllgs teystsldmw gvgcifmeml tgeptfpgvr ctydqldkif
      361 kvlgtpteet wpgvthlpgy kphrlgfypp rklglsfprl ydiaegdsma ssllqlnpdq
      421 rigaeealrh pyfaslprkl yelpdevsif svegchlytn trhgcadsrh talkt
//
LOCUS       XP_034189919             475 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189919
VERSION     XP_034189919.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334028.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="cyclin dependent kinase Eip63E isoform X4"
                     /calculated_mol_wt=53908
     Region          145..433
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(152..155,160,173,175,191,205,221..224,227,229..230,
                     270,272,274..275,277,288,291,303,305..308,310,348..349)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(152..155,160,173,175,205,221..224,227,230,274..275,
                     277,288)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(183..185,187,190..191,193..194,197..198,210,212,217,
                     259,262..265,293,295..298,302..303,419,423..425)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(191,229,270,272,291,303,305..308,310,348..349)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            287..310
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..475
                     /gene="Eip63E"
                     /coded_by="XM_034334028.2:657..2084"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mrekkggals rvqklkkrls hsfgrlsisk eeaddaanrg qlpyngysee fldrlepngn
       61 ipadkdrrye wtgvgdherv hrqlsvssds klldediree arvilrprrp prpksevflg
      121 pdppprrtkr fsafggdspf gkseayikle qlgegsyatv fkgyshltnq vvalkeirlq
      181 eeegapftai reasllkelk hsnivtlhdi ihtretltfv feyvhtdlsq ymerygsgng
      241 gldprnvklf lfqllrglay chrrrvlhrd vkpqnllise igelkladfg laraksvpsh
      301 tyshevvtlw yrppdvllgs teystsldmw gvgcifmeml tgeptfpgvr ctydqldkif
      361 kvlgtpteet wpgvthlpgy kphrlgfypp rklglsfprl ydiaegdsma ssllqlnpdq
      421 rigaeealrh pyfaslprkl yelpdevsif svegchlytn trhgcadsrh talkt
//
LOCUS       XP_034189930             417 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin dependent kinase Eip63E isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189930
VERSION     XP_034189930.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334039.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..417
                     /product="cyclin dependent kinase Eip63E isoform X5"
                     /calculated_mol_wt=47315
     Region          87..375
                     /region_name="STKc_PCTAIRE_like"
                     /note="Catalytic domain of PCTAIRE-like Serine/Threonine
                     Kinases; cd07844"
                     /db_xref="CDD:270835"
     Site            order(94..97,102,115,117,133,147,163..166,169,171..172,
                     212,214,216..217,219,230,233,245,247..250,252,290..291)
                     /site_type="active"
                     /db_xref="CDD:270835"
     Site            order(94..97,102,115,117,147,163..166,169,172,216..217,
                     219,230)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270835"
     Site            order(125..127,129,132..133,135..136,139..140,152,154,159,
                     201,204..207,235,237..240,244..245,361,365..367)
                     /site_type="other"
                     /note="putative CDK/cyclin interface [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            order(133,171,212,214,233,245,247..250,252,290..291)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270835"
     Site            229..252
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270835"
     CDS             1..417
                     /gene="Eip63E"
                     /coded_by="XM_034334039.2:127..1380"
                     /db_xref="GeneID:117608606"
ORIGIN      
        1 mkvlrtkldv iewtgvgdhe rvhrqlsvss dsklldedir eearvilrpr rpprpksevf
       61 lgpdppprrt krfsafggds pfgkseayik leqlgegsya tvfkgyshlt nqvvalkeir
      121 lqeeegapft aireasllke lkhsnivtlh diihtretlt fvfeyvhtdl sqymerygsg
      181 nggldprnvk lflfqllrgl aychrrrvlh rdvkpqnlli seigelklad fglaraksvp
      241 shtyshevvt lwyrppdvll gsteystsld mwgvgcifme mltgeptfpg vrctydqldk
      301 ifkvlgtpte etwpgvthlp gykphrlgfy pprklglsfp rlydiaegds massllqlnp
      361 dqrigaeeal rhpyfaslpr klyelpdevs ifsvegchly tntrhgcads rhtalkt
//
LOCUS       XP_034189945             126 aa            linear   INV 03-SEP-2025
DEFINITION  basic helix-loop-helix domain-containing extra-macrochaetae [Osmia
            lignaria lignaria].
ACCESSION   XP_034189945
VERSION     XP_034189945.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334054.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..126
                     /product="basic helix-loop-helix domain-containing
                     extra-macrochaetae"
                     /calculated_mol_wt=13793
     Region          34..80
                     /region_name="bHLH_dnHLH_EMC_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Drosophila melanogaster protein extra-macrochaetae and
                     similar proteins; cd19695"
                     /db_xref="CDD:381538"
     Site            order(34,37..38,41..42,44..45,48,60..61,64..65,67..68,
                     70..72,74..75,78)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381538"
     CDS             1..126
                     /gene="emc"
                     /coded_by="XM_034334054.2:501..881"
                     /db_xref="GeneID:117608630"
ORIGIN      
        1 mkamvvspvg grvppsrgil hssigingtr rdleaeevaa yltklrslvp dmprkrklsk
       61 leviqrviey icdlqttlee tnvhhesnvt kttprqplqp llnatttipt ttttttstmt
      121 gmvaer
//
LOCUS       XP_034188327            2127 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608015 [Osmia lignaria lignaria].
ACCESSION   XP_034188327
VERSION     XP_034188327.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332436.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188327.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2127
                     /product="uncharacterized protein LOC117608015"
                     /calculated_mol_wt=243355
     CDS             1..2127
                     /gene="LOC117608015"
                     /coded_by="XM_034332436.2:246..6629"
                     /db_xref="GeneID:117608015"
ORIGIN      
        1 mpsflarflg lsfrterlim niiglinstl dwsplkwtne leqiqnisls ystlilstig
       61 edriksvlsw iviiriiiah iytftyltni ypiitwsrpc lllpwlilsf fknvvleviv
      121 lavcllywyd krfslaifle fiivklvpli lasynwysns clflelryae klrklkrtlk
      181 sdtnfvtnrl cmkiadskyr trslttllsy esydtfnsqd tisrilddnd ltpaektimi
      241 lgiteqdiid aragkkered yrrltevddk lfkarkfalk kfymltkdye dqesffrpnn
      301 eelvedslvi eevplpseap tetivetlpe vtenpierse lnnsgkvdep klnnpgkvde
      361 pklnnskkvd epklnnsgei ntlkldnpik vdepklnnsg kidtpkldnp ikvdatepss
      421 neekeeinsl qknetkqnek csfsskdvea nssstqkrnc prlsnmkpet knvendcnli
      481 kfqslfncdc svddnvfkdg rahcclpdnf mlktstpnes rdtgrikenl esddlmnqnd
      541 galmlknllk ndlqssmnlg nevnskesil eeklkeevqv pnesllinya kilehenlkq
      601 evreitdavk rlfisfnedp clkgsyidek siertkdknr cgpdqnisig vqnlsevvkd
      661 mkianpveny emlttidlek skvklksane vlksmipiks pvltdinste knnsykpevr
      721 enrlrvnipe ysfgdtnipr fykdsmkvfd gcyeqdfann selqcvlqql ekkkwnpfdg
      781 qsnletllqq ddeinqstvr alrnvsspvk dcektnrfks tvhvssvntf hsenltsqkt
      841 qvtersleki pkklckfspt npiknsytqt dsvnssskin iitdsknrkk itlkrkylkd
      901 qssissmndh qnnkiktryf ansqsdkvik qsnqskssls ssdksvceck mqqletkcsp
      961 slesrredrr kssskpkvdt fdtvnlaqva rpsnfngsan krkppicpkk vkkelsssml
     1021 pkitpmirkl qeskkpktks rvessndkem ralkaynevt lvmdkkqlda krkippsktt
     1081 lnvlheeskh rlrprksnsk kklgvdkkli seeylnkenl sadasvdlkp ssvatsskvv
     1141 nesgkvlqls nevytcsklq qghtkmnrvt dkildrlfsi ekhslrkssa etdtrkqlsk
     1201 yskpkqmgde evvsassskk legmqngrel ertqakedir defkyltnss gvqpliesgk
     1261 gtkdskpqqd sllltikssv nqdvvstwss stisqleetq eaekddlpvm nqpqaildpe
     1321 dstkdptevp kvheeslplk qneirsvpee insnttnlqn ndgtrrttny tdftrnidvs
     1381 ylvhgvvlsn qntpksknpr qrdivddats rllhtafsyq smdsvldfaa dtlnvnnimn
     1441 ethldplddg fvqlsndvvd elplishdhg nleeaipaif resqdfdeqa encdllittv
     1501 deehsndvfs ivgqflrnln ignitldnvd dpglsvlqdl lrgddgeend tieeendtte
     1561 eeseedledd qtlrfikedv ftdikfpivi nwsdsliasd vdheelnpmi rdagirptgl
     1621 lclsgpeeek lkdvklecee tknveqsvff akleyssgsg klkheeknvy leeesekeie
     1681 edfgksksis sdeaaygmyr tikyfeseet aseteseenv kfeeeetivn eegamlgnve
     1741 eiikgfqlfn indtilmees meleetelpd rnaqpidfkd eeekiiliek ieeledtees
     1801 neienityiq nnqddeeeei tvlervsplt iflfwmigcl fyvqfwcrki fsaeisssts
     1861 tlnpfeihaa pvstmkllek ieekeeeedd slkeivnfdm eadfrmreqy ssniieftnd
     1921 seeiekevee amlslsehis knvkipsfln sdeektqsnl ssiasairdt etsrqnifqt
     1981 tnerlrefra aeeeiktfrf eeaddcsstk sllehneetk lsvdnqlnyp eikidedgpd
     2041 keeilendek keeekkrlks ettveeivee aesstekkqs aarvpepnfq ntdsfdstys
     2101 kisdtskrti sdlhassmee fedslns
//
LOCUS       XP_034188381            1143 aa            linear   INV 03-SEP-2025
DEFINITION  LLGL domain-containing protein l(2)gl isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188381
VERSION     XP_034188381.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332490.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1143
                     /product="LLGL domain-containing protein l(2)gl isoform
                     X1"
                     /calculated_mol_wt=125023
     Region          <32..259
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          39..77
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          83..123
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          128..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          185..228
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          235..276
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          270..372
                     /region_name="LLGL"
                     /note="LLGL2; pfam08366"
                     /db_xref="CDD:462446"
     Region          <747..948
                     /region_name="Lgl_C"
                     /note="Lethal giant larvae(Lgl) like, C-terminal;
                     pfam08596"
                     /db_xref="CDD:400768"
     CDS             1..1143
                     /gene="l(2)gl"
                     /coded_by="XM_034332490.2:658..4089"
                     /db_xref="GeneID:117608018"
ORIGIN      
        1 mlkfirgkgq qptaerqklq kdlfafrktv qhgfpnkpta lawdpslrlm iigtasgaik
       61 vfgrpgvefy gqhsiesgen avtkivalpn egrvvslcdd nslhlweine ssvvetksls
      121 legklkkisa mclessgehl llgteggniy llnlktfsmp dniiyqdvvm qnvpddykkn
      181 pgaveaiaeq pghpdnilig ynrglmvlwn katpgaqqlm glfsraqtfv stqqlesvhw
      241 isenrfvssh ndgsyafwsp isdtmvdsnt pygpfpckav tkilvyetde hgelilfsgg
      301 mqrasygdrh titvmtkkkq vvfdftskvi dfftvfpkqe dgkeipedgp ealivlaeee
      361 vvaidlinpe wkmmalpylv slhasavtcs qhvpnvpeel wdsivaagka qtehlysekp
      421 wpidggiiin qksgnekpkg remlltghed gtvrfwnasd valtplykyn ssilftgehl
      481 dvleqppedd enewppfrkv gtfdpysddp rlavkkvllc plsstlvvag taghvitati
      541 sseptnkeik aitmnivndr dgfvwkghdh lpvrttsisf avgfqpqsll qlyppaavta
      601 laihsewgll aagtahglav fdytrakavt vkctlnpndl sgagdtpisr rksfkkslre
      661 sfrrlrkgrs qrrattassp trnatekkkd vssvasspig dlspvelkpv erqvearpvd
      721 dalgsmvrcl yfarsyiism qnttptlwag tnngtvyvft laipagvrrt eedvnctlgk
      781 eiqlkhrapv iaitildgss vplpepfeae kgvspgpdma sphrvviase eqfkifnlps
      841 lkpyckyklt ahegsrvrkt gfakfscpve pagtheetcl lcltnlgdcl llsipelrrq
      901 lnaaaikred ingissltft kagealylhs sseiqrisls tskvtkanca lnlppnarsy
      961 peanneetqe qgvvegaaet egettqgsqq pttvqrtive ngvvgstdge espkespslq
     1021 paastndvng eddrqdlssi gditidsvkd hllnsslfrn atsseelhnr laglkmevts
     1081 rtseistqnq slvvktttvv sqttntttas ttvereirsg tetttthati tippnveisd
     1141 ilq
//
LOCUS       XP_076546441            1141 aa            linear   INV 03-SEP-2025
DEFINITION  LLGL domain-containing protein l(2)gl isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546441
VERSION     XP_076546441.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690326.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1141
                     /product="LLGL domain-containing protein l(2)gl isoform
                     X2"
                     /calculated_mol_wt=124849
     Region          <32..259
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          39..77
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          83..123
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          128..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          185..228
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          235..276
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          270..372
                     /region_name="LLGL"
                     /note="LLGL2; pfam08366"
                     /db_xref="CDD:462446"
     Region          <745..946
                     /region_name="Lgl_C"
                     /note="Lethal giant larvae(Lgl) like, C-terminal;
                     pfam08596"
                     /db_xref="CDD:400768"
     CDS             1..1141
                     /gene="l(2)gl"
                     /coded_by="XM_076690326.1:658..4083"
                     /db_xref="GeneID:117608018"
ORIGIN      
        1 mlkfirgkgq qptaerqklq kdlfafrktv qhgfpnkpta lawdpslrlm iigtasgaik
       61 vfgrpgvefy gqhsiesgen avtkivalpn egrvvslcdd nslhlweine ssvvetksls
      121 legklkkisa mclessgehl llgteggniy llnlktfsmp dniiyqdvvm qnvpddykkn
      181 pgaveaiaeq pghpdnilig ynrglmvlwn katpgaqqlm glfsraqtfv stqqlesvhw
      241 isenrfvssh ndgsyafwsp isdtmvdsnt pygpfpckav tkilvyetde hgelilfsgg
      301 mqrasygdrh titvmtkkkq vvfdftskvi dfftvfpkqe dgkeipedgp ealivlaeee
      361 vvaidlinpe wkmmalpylv slhasavtcs qhvpnvpeel wdsivaagka qtehlysekp
      421 wpidggiiin qksgnekpkg remlltghed gtvrfwnasd valtplykyn ssilftgehl
      481 dvleqppedd enewppfrkv gtfdpysddp rlavkkvllc plsstlvvag taghvitati
      541 sseptnkeik aitmnivndr dgfvwkghdh lpvrttsisf avgfqpqsll qlyppaavta
      601 laihsewgll aagtahglav fdytrakavt vkctlnpndl sgagdtpisr rksfkkslre
      661 sfrrlrkgrs qrrattassp trnatekkkd vvasspigdl spvelkpver qvearpvdda
      721 lgsmvrclyf arsyiismqn ttptlwagtn ngtvyvftla ipagvrrtee dvnctlgkei
      781 qlkhrapvia itildgssvp lpepfeaekg vspgpdmasp hrvviaseeq fkifnlpslk
      841 pyckykltah egsrvrktgf akfscpvepa gtheetcllc ltnlgdclll sipelrrqln
      901 aaaikredin gissltftka gealylhsss eiqrislsts kvtkancaln lppnarsype
      961 anneetqeqg vvegaaeteg ettqgsqqpt tvqrtiveng vvgstdgees pkespslqpa
     1021 astndvnged drqdlssigd itidsvkdhl lnsslfrnat sseelhnrla glkmevtsrt
     1081 seistqnqsl vvktttvvsq ttntttastt vereirsgte tttthatiti ppnveisdil
     1141 q
//
LOCUS       XP_076546442            1134 aa            linear   INV 03-SEP-2025
DEFINITION  LLGL domain-containing protein l(2)gl isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546442
VERSION     XP_076546442.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690327.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1134
                     /product="LLGL domain-containing protein l(2)gl isoform
                     X3"
                     /calculated_mol_wt=124019
     Region          <32..250
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          39..77
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          83..123
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          128..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          185..219
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          226..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..363
                     /region_name="LLGL"
                     /note="LLGL2; pfam08366"
                     /db_xref="CDD:462446"
     Region          <738..939
                     /region_name="Lgl_C"
                     /note="Lethal giant larvae(Lgl) like, C-terminal;
                     pfam08596"
                     /db_xref="CDD:400768"
     CDS             1..1134
                     /gene="l(2)gl"
                     /coded_by="XM_076690327.1:658..4062"
                     /db_xref="GeneID:117608018"
ORIGIN      
        1 mlkfirgkgq qptaerqklq kdlfafrktv qhgfpnkpta lawdpslrlm iigtasgaik
       61 vfgrpgvefy gqhsiesgen avtkivalpn egrvvslcdd nslhlweine ssvvetksls
      121 legklkkisa mclessgehl llgteggniy llnlktfsmp dniiyqdvvm qnvpddykkn
      181 pgaveaiaeq pghpdnilig ynrglmvlwn katpgaqqtf vstqqlesvh wisenrfvss
      241 hndgsyafws pisdtmvdsn tpygpfpcka vtkilvyetd ehgelilfsg gmqrasygdr
      301 htitvmtkkk qvvfdftskv idfftvfpkq edgkeipedg pealivlaee evvaidlinp
      361 ewkmmalpyl vslhasavtc sqhvpnvpee lwdsivaagk aqtehlysek pwpidggiii
      421 nqksgnekpk gremlltghe dgtvrfwnas dvaltplyky nssilftgeh ldvleqpped
      481 denewppfrk vgtfdpysdd prlavkkvll cplsstlvva gtaghvitat isseptnkei
      541 kaitmnivnd rdgfvwkghd hlpvrttsis favgfqpqsl lqlyppaavt alaihsewgl
      601 laagtahgla vfdytrakav tvkctlnpnd lsgagdtpis rrksfkkslr esfrrlrkgr
      661 sqrrattass ptrnatekkk dvssvasspi gdlspvelkp verqvearpv ddalgsmvrc
      721 lyfarsyiis mqnttptlwa gtnngtvyvf tlaipagvrr teedvnctlg keiqlkhrap
      781 viaitildgs svplpepfea ekgvspgpdm asphrvvias eeqfkifnlp slkpyckykl
      841 tahegsrvrk tgfakfscpv epagtheetc llcltnlgdc lllsipelrr qlnaaaikre
      901 dingissltf tkagealylh ssseiqrisl stskvtkanc alnlppnars ypeanneetq
      961 eqgvvegaae tegettqgsq qpttvqrtiv engvvgstdg eespkespsl qpaastndvn
     1021 geddrqdlss igditidsvk dhllnsslfr natsseelhn rlaglkmevt srtseistqn
     1081 qslvvktttv vsqttnttta sttvereirs gtetttthat itippnveis dilq
//
LOCUS       XP_076546443            1132 aa            linear   INV 03-SEP-2025
DEFINITION  LLGL domain-containing protein l(2)gl isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546443
VERSION     XP_076546443.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690328.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1132
                     /product="LLGL domain-containing protein l(2)gl isoform
                     X4"
                     /calculated_mol_wt=123845
     Region          <32..250
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          39..77
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          83..123
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          128..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          185..219
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          226..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..363
                     /region_name="LLGL"
                     /note="LLGL2; pfam08366"
                     /db_xref="CDD:462446"
     Region          <736..937
                     /region_name="Lgl_C"
                     /note="Lethal giant larvae(Lgl) like, C-terminal;
                     pfam08596"
                     /db_xref="CDD:400768"
     CDS             1..1132
                     /gene="l(2)gl"
                     /coded_by="XM_076690328.1:658..4056"
                     /db_xref="GeneID:117608018"
ORIGIN      
        1 mlkfirgkgq qptaerqklq kdlfafrktv qhgfpnkpta lawdpslrlm iigtasgaik
       61 vfgrpgvefy gqhsiesgen avtkivalpn egrvvslcdd nslhlweine ssvvetksls
      121 legklkkisa mclessgehl llgteggniy llnlktfsmp dniiyqdvvm qnvpddykkn
      181 pgaveaiaeq pghpdnilig ynrglmvlwn katpgaqqtf vstqqlesvh wisenrfvss
      241 hndgsyafws pisdtmvdsn tpygpfpcka vtkilvyetd ehgelilfsg gmqrasygdr
      301 htitvmtkkk qvvfdftskv idfftvfpkq edgkeipedg pealivlaee evvaidlinp
      361 ewkmmalpyl vslhasavtc sqhvpnvpee lwdsivaagk aqtehlysek pwpidggiii
      421 nqksgnekpk gremlltghe dgtvrfwnas dvaltplyky nssilftgeh ldvleqpped
      481 denewppfrk vgtfdpysdd prlavkkvll cplsstlvva gtaghvitat isseptnkei
      541 kaitmnivnd rdgfvwkghd hlpvrttsis favgfqpqsl lqlyppaavt alaihsewgl
      601 laagtahgla vfdytrakav tvkctlnpnd lsgagdtpis rrksfkkslr esfrrlrkgr
      661 sqrrattass ptrnatekkk dvvasspigd lspvelkpve rqvearpvdd algsmvrcly
      721 farsyiismq nttptlwagt nngtvyvftl aipagvrrte edvnctlgke iqlkhrapvi
      781 aitildgssv plpepfeaek gvspgpdmas phrvviasee qfkifnlpsl kpyckyklta
      841 hegsrvrktg fakfscpvep agtheetcll cltnlgdcll lsipelrrql naaaikredi
      901 ngissltftk agealylhss seiqrislst skvtkancal nlppnarsyp eanneetqeq
      961 gvvegaaete gettqgsqqp ttvqrtiven gvvgstdgee spkespslqp aastndvnge
     1021 ddrqdlssig ditidsvkdh llnsslfrna tsseelhnrl aglkmevtsr tseistqnqs
     1081 lvvktttvvs qttntttast tvereirsgt etttthatit ippnveisdi lq
//
LOCUS       XP_076546444            1116 aa            linear   INV 03-SEP-2025
DEFINITION  LLGL domain-containing protein l(2)gl isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546444
VERSION     XP_076546444.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690329.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1116
                     /product="LLGL domain-containing protein l(2)gl isoform
                     X5"
                     /calculated_mol_wt=121808
     Region          <5..232
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          12..50
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          56..96
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          101..144
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          158..201
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          208..249
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          243..345
                     /region_name="LLGL"
                     /note="LLGL2; pfam08366"
                     /db_xref="CDD:462446"
     Region          <720..921
                     /region_name="Lgl_C"
                     /note="Lethal giant larvae(Lgl) like, C-terminal;
                     pfam08596"
                     /db_xref="CDD:400768"
     CDS             1..1116
                     /gene="l(2)gl"
                     /coded_by="XM_076690329.1:124..3474"
                     /db_xref="GeneID:117608018"
ORIGIN      
        1 mtvqhgfpnk ptalawdpsl rlmiigtasg aikvfgrpgv efygqhsies genavtkiva
       61 lpnegrvvsl cddnslhlwe inessvvetk slslegklkk isamclessg ehlllgtegg
      121 niyllnlktf smpdniiyqd vvmqnvpddy kknpgaveai aeqpghpdni ligynrglmv
      181 lwnkatpgaq qlmglfsraq tfvstqqles vhwisenrfv sshndgsyaf wspisdtmvd
      241 sntpygpfpc kavtkilvye tdehgelilf sggmqrasyg drhtitvmtk kkqvvfdfts
      301 kvidfftvfp kqedgkeipe dgpealivla eeevvaidli npewkmmalp ylvslhasav
      361 tcsqhvpnvp eelwdsivaa gkaqtehlys ekpwpidggi iinqksgnek pkgremlltg
      421 hedgtvrfwn asdvaltply kynssilftg ehldvleqpp eddenewppf rkvgtfdpys
      481 ddprlavkkv llcplsstlv vagtaghvit atisseptnk eikaitmniv ndrdgfvwkg
      541 hdhlpvrtts isfavgfqpq sllqlyppaa vtalaihsew gllaagtahg lavfdytrak
      601 avtvkctlnp ndlsgagdtp isrrksfkks lresfrrlrk grsqrratta ssptrnatek
      661 kkdvssvass pigdlspvel kpverqvear pvddalgsmv rclyfarsyi ismqnttptl
      721 wagtnngtvy vftlaipagv rrteedvnct lgkeiqlkhr apviaitild gssvplpepf
      781 eaekgvspgp dmasphrvvi aseeqfkifn lpslkpycky kltahegsrv rktgfakfsc
      841 pvepagthee tcllcltnlg dclllsipel rrqlnaaaik redingissl tftkagealy
      901 lhssseiqri slstskvtka ncalnlppna rsypeannee tqeqgvvega aetegettqg
      961 sqqpttvqrt ivengvvgst dgeespkesp slqpaastnd vngeddrqdl ssigditids
     1021 vkdhllnssl frnatsseel hnrlaglkme vtsrtseist qnqslvvktt tvvsqttntt
     1081 tasttverei rsgtetttth atitippnve isdilq
//
LOCUS       XP_076546445            1128 aa            linear   INV 03-SEP-2025
DEFINITION  LLGL domain-containing protein l(2)gl isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546445
VERSION     XP_076546445.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690330.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1128
                     /product="LLGL domain-containing protein l(2)gl isoform
                     X6"
                     /calculated_mol_wt=123314
     Region          <32..250
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          39..77
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          83..123
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          128..171
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          185..219
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          226..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..363
                     /region_name="LLGL"
                     /note="LLGL2; pfam08366"
                     /db_xref="CDD:462446"
     Region          <738..939
                     /region_name="Lgl_C"
                     /note="Lethal giant larvae(Lgl) like, C-terminal;
                     pfam08596"
                     /db_xref="CDD:400768"
     CDS             1..1128
                     /gene="l(2)gl"
                     /coded_by="XM_076690330.1:658..4044"
                     /db_xref="GeneID:117608018"
ORIGIN      
        1 mlkfirgkgq qptaerqklq kdlfafrktv qhgfpnkpta lawdpslrlm iigtasgaik
       61 vfgrpgvefy gqhsiesgen avtkivalpn egrvvslcdd nslhlweine ssvvetksls
      121 legklkkisa mclessgehl llgteggniy llnlktfsmp dniiyqdvvm qnvpddykkn
      181 pgaveaiaeq pghpdnilig ynrglmvlwn katpgaqqtf vstqqlesvh wisenrfvss
      241 hndgsyafws pisdtmvdsn tpygpfpcka vtkilvyetd ehgelilfsg gmqrasygdr
      301 htitvmtkkk qvvfdftskv idfftvfpkq edgkeipedg pealivlaee evvaidlinp
      361 ewkmmalpyl vslhasavtc sqhvpnvpee lwdsivaagk aqtehlysek pwpidggiii
      421 nqksgnekpk gremlltghe dgtvrfwnas dvaltplyky nssilftgeh ldvleqpped
      481 denewppfrk vgtfdpysdd prlavkkvll cplsstlvva gtaghvitat isseptnkei
      541 kaitmnivnd rdgfvwkghd hlpvrttsis favgfqpqsl lqlyppaavt alaihsewgl
      601 laagtahgla vfdytrakav tvkctlnpnd lsgagdtpis rrksfkkslr esfrrlrkgr
      661 sqrrattass ptrnatekkk dvssvasspi gdlspvelkp verqvearpv ddalgsmvrc
      721 lyfarsyiis mqnttptlwa gtnngtvyvf tlaipagvrr teedvnctlg keiqlkhrap
      781 viaitildgs svplpepfea ekgvspgpdm asphrvvias eeqfkifnlp slkpyckykl
      841 tahegsrvrk tgfakfscpv epagtheetc llcltnlgdc lllsipelrr qlnaaaikre
      901 dingissltf tkagealylh ssseiqrisl stskvtkanc alnlppnars ypeanneetq
      961 eqgvvegaae tegettqgsq qpttvqrtiv engvvgstdg eespkespsl qpaastndvn
     1021 geddrqdlss igditidsvk dhllnatsse elhnrlaglk mevtsrtsei stqnqslvvk
     1081 tttvvsqttn tttasttver eirsgtettt thatitippn veisdilq
//
LOCUS       XP_034188539             636 aa            linear   INV 03-SEP-2025
DEFINITION  yellow-h isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034188539
VERSION     XP_034188539.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332648.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188539.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="yellow-h isoform X1"
                     /calculated_mol_wt=72673
     Region          330..619
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..636
                     /gene="Y-h"
                     /coded_by="XM_034332648.2:250..2160"
                     /db_xref="GeneID:117608085"
ORIGIN      
        1 mkeykllrrs tmrkrkgies kpgtlvlpdn cwissdtpkq tnnyayvsps nqfaytwdns
       61 nskrqhecvn wkvhgkysss ndgmgnvkpw llsmcllslq qfaiqpgsna lsrdletste
      121 ksvpptyrss lknyktliss hdelpghitc ispkpeenvl dtlsttpeyn nhlygstpds
      181 seyflsnfdr skfrprpsgr nlnmfkndpq llnqlethgl nydpgrgqve ddyvgpamel
      241 ifawstidyt ydsieardsa ifvgdfiaen nlplgleiwk nkvfitlpkw kdgipvtlay
      301 vpknsktksp klrpypdwgw hqpgnceglt svfrvqvdec drlwvldsgk vdvamggkqv
      361 cppaififdl ntdtlirkyi lpedqvkeds lytnivvdir nedcnsavay asdvfrygll
      421 vydffkdssf rfqhhyfypd plaskyelhg vkfqwtdgif gialspvdih ndrtlffhpm
      481 ssfrefavst silrdkktve dntdyfmpig rprakdyghs sgsvvdrngv mffnmvtrds
      541 vwcwdtrkey ipqnlgvigt snlslvfpnd ikvdheydqn vwllsnrlat ylygsvdssk
      601 vnyrvfkana keaikdtvcd pnhvvfsseh gydetc
//
LOCUS       XP_034188550             625 aa            linear   INV 03-SEP-2025
DEFINITION  yellow-h isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034188550
VERSION     XP_034188550.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332659.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188550.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..625
                     /product="yellow-h isoform X2"
                     /calculated_mol_wt=71266
     Region          319..608
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..625
                     /gene="Y-h"
                     /coded_by="XM_034332659.2:67..1944"
                     /db_xref="GeneID:117608085"
ORIGIN      
        1 mrkrkgiesk pgtlvlpdnc wissdtpkqt nnyayvspsn qfaytwdnsn skrqhecvnw
       61 kvhgkysssn dgmgnvkpwl lsmcllslqq faiqpgsnal srdletstek svpptyrssl
      121 knyktlissh delpghitci spkpeenvld tlsttpeynn hlygstpdss eyflsnfdrs
      181 kfrprpsgrn lnmfkndpql lnqlethgln ydpgrgqved dyvgpameli fawstidyty
      241 dsieardsai fvgdfiaenn lplgleiwkn kvfitlpkwk dgipvtlayv pknsktkspk
      301 lrpypdwgwh qpgnceglts vfrvqvdecd rlwvldsgkv dvamggkqvc ppaififdln
      361 tdtlirkyil pedqvkedsl ytnivvdirn edcnsavaya sdvfrygllv ydffkdssfr
      421 fqhhyfypdp laskyelhgv kfqwtdgifg ialspvdihn drtlffhpms sfrefavsts
      481 ilrdkktved ntdyfmpigr prakdyghss gsvvdrngvm ffnmvtrdsv wcwdtrkeyi
      541 pqnlgvigts nlslvfpndi kvdheydqnv wllsnrlaty lygsvdsskv nyrvfkanak
      601 eaikdtvcdp nhvvfssehg ydetc
//
LOCUS       XP_034188583             626 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188583
VERSION     XP_034188583.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332692.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..626
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X1"
                     /calculated_mol_wt=70966
     Region          <41..>122
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          59..84
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          89..122
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          229..547
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(244..245,480..485,493..495,516..519)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(244..245,481..485,518)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..626
                     /gene="LOC117608096"
                     /coded_by="XM_034332692.2:615..2495"
                     /db_xref="GeneID:117608096"
ORIGIN      
        1 mdiakeliln lkqnnkshvg yglqkeceal ighvlqnmvq selpelipev kheqdsiqyr
       61 eegnqhfvmg ddieaikcyt mslayannke lmsyahanrs aalyrkqmyk eciididaal
      121 nlgypeekre klkergtkav eeikkkllik kddhidtekl mnaclsekis dspnvvkyrn
      181 gkeeigkkin knvnadenkh dsvneekepr yladegplkl aygpgkeapa asdgvsiafs
      241 ekygrhlvat kefkpgdivt iedpyacily aqcyythchq clarcynlip ctkcpvaqyc
      301 setcrklawe mahqiecpil aivgnllnvd edkirmltki irflivvtak gekieelled
      361 mkiaesnpdn rtagftdegi ldsasarsal slatnmttrp ligisafaci sslaimllat
      421 qtnffckkye vnqlknidkf pkirfcgslm fracvimssn cfsvqqdpgi ktgsglyvth
      481 slynhscapn tfrhfegltm itralqpisp gdqiftnyga syaymtrsar rekimqdyff
      541 ecdctactfd wptyntilrn higsisknke lveklkpykq rltknmydie avksvllilf
      601 kvvtqpceev vhaeqylksy ylgkfk
//
LOCUS       XP_034188592             624 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188592
VERSION     XP_034188592.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332701.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..624
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X2"
                     /calculated_mol_wt=70718
     Region          <41..>122
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          59..84
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          89..122
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          229..547
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Site            order(244..245,480..485,493..495,516..519)
                     /site_type="active"
                     /db_xref="CDD:380914"
     Site            order(244..245,481..485,518)
                     /site_type="other"
                     /note="SAM binding site [polypeptide binding]"
                     /db_xref="CDD:380914"
     CDS             1..624
                     /gene="LOC117608096"
                     /coded_by="XM_034332701.2:615..2489"
                     /db_xref="GeneID:117608096"
ORIGIN      
        1 mdiakeliln lkqnnkshvg yglqkeceal ighvlqnmvq selpelipev kheqdsiqyr
       61 eegnqhfvmg ddieaikcyt mslayannke lmsyahanrs aalyrkqmyk eciididaal
      121 nlgypeekre klkergtkav eeikkkllik kddhidtekl mnaclsekis dspnvvkyrn
      181 gkeeigkkin knvnadenkh dsvneekepr yladegplkl aygpgkeapa asdgvsiafs
      241 ekygrhlvat kefkpgdivt iedpyacily aqcyythchq clarcynlip ctkcpvaqyc
      301 setcrklawe mahqiecpil aivgnllnvd edkirmltki irflivvtak gekieelled
      361 mkiaesnpdn rtagftdegi ldsasarsal slatnmttrp ligisafaci sslaimllat
      421 qtnffckkye vnqlknidkf pkirfcgslm fracvimssn cfsvqqdpgi ktgsglyvth
      481 slynhscapn tfrhfegltm itralqpisp gdqiftnyga syaymtrsar rekimqdyff
      541 ecdctactfd wptyntilrn higsisknke lveklkpykq rltknmydie avksvllilf
      601 kvvtqpceev vhaeqylksy yldp
//
LOCUS       XP_034188667             586 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034188667
VERSION     XP_034188667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="epsin homolog lqf isoform X1"
                     /calculated_mol_wt=63856
     Region          37..159
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(43,47..48,81,87,91)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <302..583
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..586
                     /gene="lqf"
                     /coded_by="XM_034332776.2:419..2179"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mptmamqkmr rqgqdvmqvn lagirrdivn lahnysnaqk avrkatsndp wgpsstlmae
       61 iadltynvva fteimqmlwk rlndhgknwr hvykalille yliktgtekv aqqckenifa
      121 iqtlkdfqym egskdqgvyv rekakqlval lkdderlrne raralkaker faqsvsgfgs
      181 dgldtmspvs sdfqdwepcr lgsestarrt skpeleaarp qtvgeeelql qlalamsree
      241 aeqeeqrrrs ddvrlqlals qsqqdfkapq tekqshmldl ldvnleeacg ynvktdpwgi
      301 pitappprpq qqndpwsapt tsstspaidp wtpvppqrpt aavdpwrapp sspvtvtssp
      361 stdpwsplps atatatttte adankkqadr egitstspaf nnstltqnig faaqspqnig
      421 fnlpttssat fnatsngfng tgpslnnfpl gnqnssaasp lndldefdii tnrnkpgssl
      481 qtanngdtls pdpfdlgsia dnlptstgav kktpqsflge nsalvnldnl vststikptt
      541 ptpaaaitys anpfatatpp prptinqirq dpwaantttt anpfls
//
LOCUS       XP_076546446             586 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546446
VERSION     XP_076546446.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690331.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="epsin homolog lqf isoform X1"
                     /calculated_mol_wt=63856
     Region          37..159
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(43,47..48,81,87,91)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <302..583
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..586
                     /gene="lqf"
                     /coded_by="XM_076690331.1:261..2021"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mptmamqkmr rqgqdvmqvn lagirrdivn lahnysnaqk avrkatsndp wgpsstlmae
       61 iadltynvva fteimqmlwk rlndhgknwr hvykalille yliktgtekv aqqckenifa
      121 iqtlkdfqym egskdqgvyv rekakqlval lkdderlrne raralkaker faqsvsgfgs
      181 dgldtmspvs sdfqdwepcr lgsestarrt skpeleaarp qtvgeeelql qlalamsree
      241 aeqeeqrrrs ddvrlqlals qsqqdfkapq tekqshmldl ldvnleeacg ynvktdpwgi
      301 pitappprpq qqndpwsapt tsstspaidp wtpvppqrpt aavdpwrapp sspvtvtssp
      361 stdpwsplps atatatttte adankkqadr egitstspaf nnstltqnig faaqspqnig
      421 fnlpttssat fnatsngfng tgpslnnfpl gnqnssaasp lndldefdii tnrnkpgssl
      481 qtanngdtls pdpfdlgsia dnlptstgav kktpqsflge nsalvnldnl vststikptt
      541 ptpaaaitys anpfatatpp prptinqirq dpwaantttt anpfls
//
LOCUS       XP_076546447             584 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546447
VERSION     XP_076546447.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690332.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..584
                     /product="epsin homolog lqf isoform X2"
                     /calculated_mol_wt=63581
     Region          37..159
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(43,47..48,81,87,91)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <300..581
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..584
                     /gene="lqf"
                     /coded_by="XM_076690332.1:418..2172"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mptmamqkmr rqgqdvmqvn lagirrdivn lahnysnaqk avrkatsndp wgpsstlmae
       61 iadltynvva fteimqmlwk rlndhgknwr hvykalille yliktgtekv aqqckenifa
      121 iqtlkdfqym egskdqgvyv rekakqlval lkdderlrne raralkaker faqsvsgfgs
      181 dgldtmspvs sddwepcrlg sestarrtsk peleaarpqt vgeeelqlql alamsreeae
      241 qeeqrrrsdd vrlqlalsqs qqdfkapqte kqshmldlld vnleeacgyn vktdpwgipi
      301 tappprpqqq ndpwsaptts stspaidpwt pvppqrptaa vdpwrappss pvtvtsspst
      361 dpwsplpsat atattttead ankkqadreg itstspafnn stltqnigfa aqspqnigfn
      421 lpttssatfn atsngfngtg pslnnfplgn qnssaaspln dldefdiitn rnkpgsslqt
      481 anngdtlspd pfdlgsiadn lptstgavkk tpqsflgens alvnldnlvs tstikpttpt
      541 paaaitysan pfatatpppr ptinqirqdp waanttttan pfls
//
LOCUS       XP_076546448             578 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546448
VERSION     XP_076546448.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690333.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="epsin homolog lqf isoform X3"
                     /calculated_mol_wt=62937
     Region          29..151
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(35,39..40,73,79,83)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <294..575
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..578
                     /gene="lqf"
                     /coded_by="XM_076690333.1:353..2089"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mrrqgqdvmq vnlagirrdi vnlahnysna qkavrkatsn dpwgpsstlm aeiadltynv
       61 vafteimqml wkrlndhgkn wrhvykalil leyliktgte kvaqqckeni faiqtlkdfq
      121 ymegskdqgv yvrekakqlv allkdderlr neraralkak erfaqsvsgf gsdgldtmsp
      181 vssdfqdwep crlgsestar rtskpeleaa rpqtvgeeel qlqlalamsr eeaeqeeqrr
      241 rsddvrlqla lsqsqqdfka pqtekqshml dlldvnleea cgynvktdpw gipitapppr
      301 pqqqndpwsa pttsstspai dpwtpvppqr ptaavdpwra ppsspvtvts spstdpwspl
      361 psatatattt teadankkqa dregitstsp afnnstltqn igfaaqspqn igfnlpttss
      421 atfnatsngf ngtgpslnnf plgnqnssaa splndldefd iitnrnkpgs slqtanngdt
      481 lspdpfdlgs iadnlptstg avkktpqsfl gensalvnld nlvststikp ttptpaaait
      541 ysanpfatat ppprptinqi rqdpwaantt ttanpfls
//
LOCUS       XP_076546449             578 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546449
VERSION     XP_076546449.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690334.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="epsin homolog lqf isoform X3"
                     /calculated_mol_wt=62937
     Region          29..151
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(35,39..40,73,79,83)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <294..575
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..578
                     /gene="lqf"
                     /coded_by="XM_076690334.1:195..1931"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mrrqgqdvmq vnlagirrdi vnlahnysna qkavrkatsn dpwgpsstlm aeiadltynv
       61 vafteimqml wkrlndhgkn wrhvykalil leyliktgte kvaqqckeni faiqtlkdfq
      121 ymegskdqgv yvrekakqlv allkdderlr neraralkak erfaqsvsgf gsdgldtmsp
      181 vssdfqdwep crlgsestar rtskpeleaa rpqtvgeeel qlqlalamsr eeaeqeeqrr
      241 rsddvrlqla lsqsqqdfka pqtekqshml dlldvnleea cgynvktdpw gipitapppr
      301 pqqqndpwsa pttsstspai dpwtpvppqr ptaavdpwra ppsspvtvts spstdpwspl
      361 psatatattt teadankkqa dregitstsp afnnstltqn igfaaqspqn igfnlpttss
      421 atfnatsngf ngtgpslnnf plgnqnssaa splndldefd iitnrnkpgs slqtanngdt
      481 lspdpfdlgs iadnlptstg avkktpqsfl gensalvnld nlvststikp ttptpaaait
      541 ysanpfatat ppprptinqi rqdpwaantt ttanpfls
//
LOCUS       XP_076546450             578 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546450
VERSION     XP_076546450.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690335.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..578
                     /product="epsin homolog lqf isoform X3"
                     /calculated_mol_wt=62937
     Region          29..151
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(35,39..40,73,79,83)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <294..575
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..578
                     /gene="lqf"
                     /coded_by="XM_076690335.1:186..1922"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mrrqgqdvmq vnlagirrdi vnlahnysna qkavrkatsn dpwgpsstlm aeiadltynv
       61 vafteimqml wkrlndhgkn wrhvykalil leyliktgte kvaqqckeni faiqtlkdfq
      121 ymegskdqgv yvrekakqlv allkdderlr neraralkak erfaqsvsgf gsdgldtmsp
      181 vssdfqdwep crlgsestar rtskpeleaa rpqtvgeeel qlqlalamsr eeaeqeeqrr
      241 rsddvrlqla lsqsqqdfka pqtekqshml dlldvnleea cgynvktdpw gipitapppr
      301 pqqqndpwsa pttsstspai dpwtpvppqr ptaavdpwra ppsspvtvts spstdpwspl
      361 psatatattt teadankkqa dregitstsp afnnstltqn igfaaqspqn igfnlpttss
      421 atfnatsngf ngtgpslnnf plgnqnssaa splndldefd iitnrnkpgs slqtanngdt
      481 lspdpfdlgs iadnlptstg avkktpqsfl gensalvnld nlvststikp ttptpaaait
      541 ysanpfatat ppprptinqi rqdpwaantt ttanpfls
//
LOCUS       XP_076546451             566 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546451
VERSION     XP_076546451.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690336.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..566
                     /product="epsin homolog lqf isoform X4"
                     /calculated_mol_wt=61764
     Region          37..159
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(43,47..48,81,87,91)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          301..>369
                     /region_name="FAP"
                     /note="Fibronectin-attachment protein (FAP); pfam07174"
                     /db_xref="CDD:429334"
     Region          <302..563
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..566
                     /gene="lqf"
                     /coded_by="XM_076690336.1:419..2119"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mptmamqkmr rqgqdvmqvn lagirrdivn lahnysnaqk avrkatsndp wgpsstlmae
       61 iadltynvva fteimqmlwk rlndhgknwr hvykalille yliktgtekv aqqckenifa
      121 iqtlkdfqym egskdqgvyv rekakqlval lkdderlrne raralkaker faqsvsgfgs
      181 dgldtmspvs sdfqdwepcr lgsestarrt skpeleaarp qtvgeeelql qlalamsree
      241 aeqeeqrrrs ddvrlqlals qsqqdfkapq tekqshmldl ldvnleeacg ynvktdpwgi
      301 pitappprpq qqndpwsapt tsstspaidp wtpvppqrpt aavdpwrapp sspvtvtssp
      361 stdpwsplps atatatttte adankkqnig faaqspqnig fnlpttssat fnatsngfng
      421 tgpslnnfpl gnqnssaasp lndldefdii tnrnkpgssl qtanngdtls pdpfdlgsia
      481 dnlptstgav kktpqsflge nsalvnldnl vststikptt ptpaaaitys anpfatatpp
      541 prptinqirq dpwaantttt anpfls
//
LOCUS       XP_076546452             559 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546452
VERSION     XP_076546452.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690337.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..559
                     /product="epsin homolog lqf isoform X5"
                     /calculated_mol_wt=61062
     Region          37..159
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(43,47..48,81,87,91)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <285..556
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          301..>369
                     /region_name="FAP"
                     /note="Fibronectin-attachment protein (FAP); pfam07174"
                     /db_xref="CDD:429334"
     CDS             1..559
                     /gene="lqf"
                     /coded_by="XM_076690337.1:419..2098"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mptmamqkmr rqgqdvmqvn lagirrdivn lahnysnaqk avrkatsndp wgpsstlmae
       61 iadltynvva fteimqmlwk rlndhgknwr hvykalille yliktgtekv aqqckenifa
      121 iqtlkdfqym egskdqgvyv rekakqlval lkdderlrne raralkaker faqsvsgfgs
      181 dgldtmspvs sdfqdwepcr lgsestarrt skpeleaarp qtvgeeelql qlalamsree
      241 aeqeeqrrrs ddvrlqlals qsqqdfkapq tekqshmldl ldvnleeacg ynvktdpwgi
      301 pitappprpq qqndpwsapt tsstspaidp wtpvppqrpt aavdpwrapp sspvtvtssp
      361 stdpwsplps atatatttte adankkqspq nigfnlptts satfnatsng fngtgpslnn
      421 fplgnqnssa asplndldef diitnrnkpg sslqtanngd tlspdpfdlg siadnlptst
      481 gavkktpqsf lgensalvnl dnlvststik pttptpaaai tysanpfata tppprptinq
      541 irqdpwaant tttanpfls
//
LOCUS       XP_076546453             552 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076546453
VERSION     XP_076546453.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690338.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..552
                     /product="epsin homolog lqf isoform X6"
                     /calculated_mol_wt=60186
     Region          37..159
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(43,47..48,81,87,91)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <267..>544
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          301..>369
                     /region_name="FAP"
                     /note="Fibronectin-attachment protein (FAP); pfam07174"
                     /db_xref="CDD:429334"
     CDS             1..552
                     /gene="lqf"
                     /coded_by="XM_076690338.1:421..2079"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mptmamqkmr rqgqdvmqvn lagirrdivn lahnysnaqk avrkatsndp wgpsstlmae
       61 iadltynvva fteimqmlwk rlndhgknwr hvykalille yliktgtekv aqqckenifa
      121 iqtlkdfqym egskdqgvyv rekakqlval lkdderlrne raralkaker faqsvsgfgs
      181 dgldtmspvs sdfqdwepcr lgsestarrt skpeleaarp qtvgeeelql qlalamsree
      241 aeqeeqrrrs ddvrlqlals qsqqdfkapq tekqshmldl ldvnleeacg ynvktdpwgi
      301 pitappprpq qqndpwsapt tsstspaidp wtpvppqrpt aavdpwrapp sspvtvtssp
      361 stdpwsplps atatatttte adankkqadr egitstspaf nnstltqnig faaqspqnig
      421 fnlpttssat fnatsngfng tgpslnnfpl gnqnssaasp lndldefdii tnrnkpgssl
      481 qtanngdtls pdpfdlgsia dnlptstgav kktpqsflge nsalvnldnl vststikptt
      541 ptpavldgln eg
//
LOCUS       XP_076546454             549 aa            linear   INV 03-SEP-2025
DEFINITION  epsin homolog lqf isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546454
VERSION     XP_076546454.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690339.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..549
                     /product="epsin homolog lqf isoform X7"
                     /calculated_mol_wt=60058
     Region          37..159
                     /region_name="ENTH_Epsin"
                     /note="Epsin N-Terminal Homology (ENTH) domain of Epsin
                     family; cd16990"
                     /db_xref="CDD:340787"
     Site            order(43,47..48,81,87,91)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340787"
     Region          <267..>544
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          301..>369
                     /region_name="FAP"
                     /note="Fibronectin-attachment protein (FAP); pfam07174"
                     /db_xref="CDD:429334"
     CDS             1..549
                     /gene="lqf"
                     /coded_by="XM_076690339.1:421..2070"
                     /db_xref="GeneID:117608108"
ORIGIN      
        1 mptmamqkmr rqgqdvmqvn lagirrdivn lahnysnaqk avrkatsndp wgpsstlmae
       61 iadltynvva fteimqmlwk rlndhgknwr hvykalille yliktgtekv aqqckenifa
      121 iqtlkdfqym egskdqgvyv rekakqlval lkdderlrne raralkaker faqsvsgfgs
      181 dgldtmspvs sdfqdwepcr lgsestarrt skpeleaarp qtvgeeelql qlalamsree
      241 aeqeeqrrrs ddvrlqlals qsqqdfkapq tekqshmldl ldvnleeacg ynvktdpwgi
      301 pitappprpq qqndpwsapt tsstspaidp wtpvppqrpt aavdpwrapp sspvtvtssp
      361 stdpwsplps atatatttte adankkqadr egitstspaf nnstltqnig faaqspqnig
      421 fnlpttssat fnatsngfng tgpslnnfpl gnqnssaasp lndldefdii tnrnkpgssl
      481 qtanngdtls pdpfdlgsia dnlptstgav kktpqsflge nsalvnldnl vststikptt
      541 ptpaafhwk
//
LOCUS       XP_034188715             474 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034188715
VERSION     XP_034188715.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332824.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="casein kinase I isoform X1"
                     /calculated_mol_wt=54116
     Region          8..282
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..474
                     /gene="LOC117608159"
                     /coded_by="XM_034332824.2:860..2284"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypvefas ylrycrglrf eerpdysylr qlfrtlfhge gftydyvfdw nmlkfgnvrq
      301 qtlsstqqap mqsqhtnaal psgtnndqeh rsrpytrqcl anasvgtvgp tvggtatnlr
      361 slrqkreaid alrdqdnqdk sdlqasgagl qerrvsmrlh rrdaaagemq pktkqnhhrr
      421 lddgcraskv gharnrellq adkaaklktn rslhlpipfe pnlkktsqsq aplr
//
LOCUS       XP_034188725             473 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034188725
VERSION     XP_034188725.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332834.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="casein kinase I isoform X2"
                     /calculated_mol_wt=54017
     Region          8..281
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..473
                     /gene="LOC117608159"
                     /coded_by="XM_034332834.2:863..2284"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypefasy lrycrglrfe erpdysylrq lfrtlfhgeg ftydyvfdwn mlkfgnvrqq
      301 tlsstqqapm qsqhtnaalp sgtnndqehr srpytrqcla nasvgtvgpt vggtatnlrs
      361 lrqkreaida lrdqdnqdks dlqasgaglq errvsmrlhr rdaaagemqp ktkqnhhrrl
      421 ddgcraskvg harnrellqa dkaaklktnr slhlpipfep nlkktsqsqa plr
//
LOCUS       XP_034188735             448 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034188735
VERSION     XP_034188735.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332844.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..448
                     /product="casein kinase I isoform X3"
                     /calculated_mol_wt=51140
     Region          8..282
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..448
                     /gene="LOC117608159"
                     /coded_by="XM_034332844.2:860..2206"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypvefas ylrycrglrf eerpdysylr qlfrtlfhge gftydyvfdw nmlkfgnvrq
      301 qtlsstqqap mqsqhtnaal psgtnndqeh rsrpytrqcl anasvgtvgp tvggtatnlr
      361 slrqkreaid alrdqdnqdk sdlqasgagl qerrvsmrlh rrdaaagemq pktksvtkek
      421 ergkgsrmmi tkfclatgil twfhrsta
//
LOCUS       XP_034188744             429 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034188744
VERSION     XP_034188744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..429
                     /product="casein kinase I isoform X4"
                     /calculated_mol_wt=49090
     Region          8..282
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..429
                     /gene="LOC117608159"
                     /coded_by="XM_034332853.2:858..2147"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypvefas ylrycrglrf eerpdysylr qlfrtlfhge gftydyvfdw nmlkfgnvrq
      301 qtlsstqqap mqsqhtnaal psgtnndqeh rsrpytrqcl anasvgtvgp tvggtatnlr
      361 slrqkreaid alrdqdnqdk sdlqasgagl qerrvsmrlh rrdaaagemq pktkiqrvlt
      421 aeptqryft
//
LOCUS       XP_034188752             421 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034188752
VERSION     XP_034188752.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332861.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="casein kinase I isoform X5"
                     /calculated_mol_wt=48095
     Region          8..282
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..421
                     /gene="LOC117608159"
                     /coded_by="XM_034332861.2:857..2122"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypvefas ylrycrglrf eerpdysylr qlfrtlfhge gftydyvfdw nmlkfgnvrq
      301 qtlsstqqap mqsqhtnaal psgtnndqeh rsrpytrqcl anasvgtvgp tvggtatnlr
      361 slrqkreaid alrdqdnqdk sdlqasgagl qerrvsmrlh rrdaaagemq pktklgrvqq
      421 k
//
LOCUS       XP_034188761             421 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034188761
VERSION     XP_034188761.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332870.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="casein kinase I isoform X6"
                     /calculated_mol_wt=47990
     Region          8..282
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..421
                     /gene="LOC117608159"
                     /coded_by="XM_034332870.2:857..2122"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypvefas ylrycrglrf eerpdysylr qlfrtlfhge gftydyvfdw nmlkfgnvrq
      301 qtlsstqqap mqsqhtnaal psgtnndqeh rsrpytrqcl anasvgtvgp tvggtatnlr
      361 slrqkreaid alrdqdnqdk sdlqasgagl qerrvsmrlh rrdaaagemq pktklmksts
      421 g
//
LOCUS       XP_034188771             415 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034188771
VERSION     XP_034188771.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332880.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..415
                     /product="casein kinase I isoform X7"
                     /calculated_mol_wt=47370
     Region          8..282
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..415
                     /gene="LOC117608159"
                     /coded_by="XM_034332880.2:857..2104"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypvefas ylrycrglrf eerpdysylr qlfrtlfhge gftydyvfdw nmlkfgnvrq
      301 qtlsstqqap mqsqhtnaal psgtnndqeh rsrpytrqcl anasvgtvgp tvggtatnlr
      361 slrqkreaid alrdqdnqdk sdlqasgagl qerrvsmrlh rrdaaagemq pktnv
//
LOCUS       XP_034188779             414 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034188779
VERSION     XP_034188779.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332888.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="casein kinase I isoform X8"
                     /calculated_mol_wt=47285
     Region          8..282
                     /region_name="STKc_CK1_delta_epsilon"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinases, Casein Kinase 1 delta and epsilon; cd14125"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,88,90,128,130,132..133,135,
                     149,152,175..178)
                     /site_type="active"
                     /db_xref="CDD:271027"
     Site            order(15..21,23,36,38,82..86,130,132..133,135,149)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271027"
     Site            order(19,88,90,128,130,132,152,175..178)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271027"
     Site            order(148..158,160..178)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271027"
     CDS             1..414
                     /gene="LOC117608159"
                     /coded_by="XM_034332888.2:857..2101"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 melrvgnkyr lgrkigsgsf gdiylgtnis tgeevaikle ciktrhpqlh ieskfykmmq
       61 ggvgiptikw cgsegdynvm vmellgpsle dlfnfcsrrf slktvlllad qlicrtdyih
      121 srnfihrdik pdnflmglgk kgnlvyiidf glakkyrdgr thkhipyren knltgtarya
      181 sinthlgieq srrddleslg yvlmyfnrgs lpwqglkaat krqkyerise kkmstpveel
      241 ckgypvefas ylrycrglrf eerpdysylr qlfrtlfhge gftydyvfdw nmlkfgnvrq
      301 qtlsstqqap mqsqhtnaal psgtnndqeh rsrpytrqcl anasvgtvgp tvggtatnlr
      361 slrqkreaid alrdqdnqdk sdlqasgagl qerrvsmrlh rrdaaagemq pktk
//
LOCUS       XP_076546455             339 aa            linear   INV 03-SEP-2025
DEFINITION  casein kinase I isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_076546455
VERSION     XP_076546455.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690340.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="casein kinase I isoform X9"
                     /calculated_mol_wt=38687
     Region          <1..147
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..339
                     /gene="LOC117608159"
                     /coded_by="XM_076690340.1:232..1251"
                     /db_xref="GeneID:117608159"
ORIGIN      
        1 mglgkkgnlv yiidfglakk yrdgrthkhi pyrenknltg taryasinth lgieqsrrdd
       61 leslgyvlmy fnrgslpwqg lkaatkrqky erisekkmst pveelckgyp vefasylryc
      121 rglrfeerpd ysylrqlfrt lfhgegftyd yvfdwnmlkf gnvrqqtlss tqqapmqsqh
      181 tnaalpsgtn ndqehrsrpy trqclanasv gtvgptvggt atnlrslrqk reaidalrdq
      241 dnqdksdlqa sgaglqerrv smrlhrrdaa agemqpktkq nhhrrlddgc raskvgharn
      301 rellqadkaa klktnrslhl pipfepnlkk tsqsqaplr
//
LOCUS       XP_076546456             435 aa            linear   INV 03-SEP-2025
DEFINITION  yellow-e3 [Osmia lignaria lignaria].
ACCESSION   XP_076546456
VERSION     XP_076546456.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690341.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..435
                     /product="yellow-e3"
                     /calculated_mol_wt=49217
     Region          130..421
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..435
                     /gene="Y-e3"
                     /coded_by="XM_076690341.1:199..1506"
                     /db_xref="GeneID:143305704"
ORIGIN      
        1 meiitllrfh skmrlcwtil fliifdarah eklkiiyswk aleflfpnqf akevaiknge
       61 fipgapvpid vdvyngghqs tvfvaiprfq kgvpvtlgyv tdkvstdgnp liapfpnwey
      121 naigncdmit svyrmqidqc drlwvldtgv veetkvcppk lhvfslrkgt litrytfprd
      181 hykedslhvt vavdvrnpeh dckdtfayia dvtgfalivy dhrnsrswki nnnlfypypp
      241 hgtfnikdvt fdlmdgilgl avgplrnddr ilyfhslasr vesrvstsvi rnytlfrdnp
      301 eaaarsfiay egerasqsva qamdrrgvlf fgllsdlaig cwnskhfpny ggpnneilvt
      361 dsetlqfpsg lkiiiskkgk qelwvlsasf qkymsgslhs netnfiiqag fvdelvrgtk
      421 cdvsaldsrg fqfpk
//
LOCUS       XP_034181033             409 aa            linear   INV 03-SEP-2025
DEFINITION  dopaminechrome tautomerase [Osmia lignaria lignaria].
ACCESSION   XP_034181033
VERSION     XP_034181033.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181033.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="dopaminechrome tautomerase"
                     /calculated_mol_wt=46397
     Region          110..378
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..409
                     /gene="LOC117604731"
                     /coded_by="XM_034325142.2:111..1340"
                     /db_xref="GeneID:117604731"
ORIGIN      
        1 mrilvfvlla gfssaevlqt iaqwplmdfa lpydrgflnq yrpenvvptg ieigwdkifv
       61 tiprlragvp stlnyiprnl plesspqlqa ypswdwhsag kgdlncskli svyrsrldrc
      121 nrlwvldsgv mtsiddfmpv cmpkimvfdl qtdqlvrqyt fprevlrpnt lltnliiddv
      181 sattcddvfi ymsdtvgpgi lvfdgatdrs wrvmhasmyp hpdysmyrig sdtfelmdgv
      241 vglafsakqg lvyyqplatd rlfsvpstal qagppafgeq lpvtlvgrks sqglalvvdp
      301 rddtilfspl tevaiaawqp qtnrqrilay speklqfvae irwadhdkgn fwimstrfqk
      361 ffrkevnvrd inirimrltp vqlplkhail nsytqssfpl nfynntlgf
//
LOCUS       XP_076546457             407 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305705 [Osmia lignaria lignaria].
ACCESSION   XP_076546457
VERSION     XP_076546457.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690342.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..407
                     /product="uncharacterized protein LOC143305705"
                     /calculated_mol_wt=46921
     CDS             1..407
                     /gene="LOC143305705"
                     /coded_by="XM_076690342.1:139..1362"
                     /db_xref="GeneID:143305705"
ORIGIN      
        1 msrdysanrp iegspnasfr qldkqsyffq syktetdidr emmmfdrsvl dekkidyade
       61 evaaasggsl ncfgglekek gcndhdekkq kssmavgrhh atthdtrrds gnveflekeg
      121 eifkisssel lqessgkkkr nfsfnnkekn hrsleslers idlsvmdcks ldllseeqpk
      181 tfyhgnqrsl dssqkskksm dssvnktrkr pnffqrvrrs whfllmqrri rqrnkyerpp
      241 sgrekceyfl qrlhkndfie rlsslpinpp rvqtsvdffp erdtskdqvi ltdvnddkef
      301 tvqlenstfr vndeekdlpd entnmltren eaegcsltge ndvslktqei vdnekkeqfm
      361 ewvvtianld alshgthsnd sigcgilhgt lgilksilfd iflcici
//
LOCUS       XP_076546458             405 aa            linear   INV 03-SEP-2025
DEFINITION  major royal jelly protein 9 [Osmia lignaria lignaria].
ACCESSION   XP_076546458
VERSION     XP_076546458.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690343.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="major royal jelly protein 9"
                     /calculated_mol_wt=45651
     Region          119..397
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..405
                     /gene="Mrjp9"
                     /coded_by="XM_076690343.1:169..1386"
                     /db_xref="GeneID:143305706"
ORIGIN      
        1 mkaswllllc faavsfqnav avskkeamkv ikewkymdyk ydseeqkqsa insgeynykn
       61 cfpidvdrwn gktfvtvird egvpsslntv skemgkggpl ltpypdwswa stsscnniis
      121 vyrvaidrcd rlwvldtgvk gsdricpakl lifylptskl ikrvtipdni amntttgqgl
      181 lvtpvvqtfg rgcqktfvyi sdveghglif ydgssfrrit staldydpka tvytiqkesf
      241 tlqdgpvgma lspisgniyy spmssfnlds vntrsivesg gtdvwfkeyk nilwtqssak
      301 amsdtgilff glvnntsigc wneyrqlrrp nidvvalnee nlqftsglkv kscagreely
      361 amtnryqkia tgsinfnqin frilkgdvft liqntrcqpe ryryy
//
LOCUS       XP_076546459             405 aa            linear   INV 03-SEP-2025
DEFINITION  major royal jelly protein 9 [Osmia lignaria lignaria].
ACCESSION   XP_076546459
VERSION     XP_076546459.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690344.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="major royal jelly protein 9"
                     /calculated_mol_wt=45651
     Region          119..397
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..405
                     /gene="Mrjp9"
                     /coded_by="XM_076690344.1:91..1308"
                     /db_xref="GeneID:143305706"
ORIGIN      
        1 mkaswllllc faavsfqnav avskkeamkv ikewkymdyk ydseeqkqsa insgeynykn
       61 cfpidvdrwn gktfvtvird egvpsslntv skemgkggpl ltpypdwswa stsscnniis
      121 vyrvaidrcd rlwvldtgvk gsdricpakl lifylptskl ikrvtipdni amntttgqgl
      181 lvtpvvqtfg rgcqktfvyi sdveghglif ydgssfrrit staldydpka tvytiqkesf
      241 tlqdgpvgma lspisgniyy spmssfnlds vntrsivesg gtdvwfkeyk nilwtqssak
      301 amsdtgilff glvnntsigc wneyrqlrrp nidvvalnee nlqftsglkv kscagreely
      361 amtnryqkia tgsinfnqin frilkgdvft liqntrcqpe ryryy
//
LOCUS       XP_034188792             376 aa            linear   INV 03-SEP-2025
DEFINITION  L-dopachrome tautomerase yellow-g [Osmia lignaria lignaria].
ACCESSION   XP_034188792
VERSION     XP_034188792.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332901.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188792.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="L-dopachrome tautomerase yellow-g"
                     /calculated_mol_wt=40802
     Region          118..373
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..376
                     /gene="yellow-g"
                     /coded_by="XM_034332901.2:715..1845"
                     /db_xref="GeneID:117608189"
ORIGIN      
        1 mkrlcitlcl mvlvkdsllt yttqdvsqsl vlsgqslnwp cqstkniyet sgryitrnvi
       61 atraqvykne ailalprykp gvpftlgvls lkssdcapki tpfpcwaiqe egncqalqsa
      121 vdialdmqgi lwvldvgivn tleqpvrrcp pkvvgvnans gkvvkvidls sladtssrlq
      181 ymavdyaadg qvyvyvsdag traiivynvt adsgyrvvlp savtsgmdkp dalylalirk
      241 scgtqvlyft ylgssrmfai kasnlrtgna ngsivdiggk knkivllgtd ngsaiffrik
      301 gdsnvymwns etpfvsdnfl lvqkagdcrl ptgvipgykg lmwviesnfq dyidnavscs
      361 gasvslhplv nsceey
//
LOCUS       XP_034188799             376 aa            linear   INV 03-SEP-2025
DEFINITION  L-dopachrome tautomerase yellow-g [Osmia lignaria lignaria].
ACCESSION   XP_034188799
VERSION     XP_034188799.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332908.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188799.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="L-dopachrome tautomerase yellow-g"
                     /calculated_mol_wt=40802
     Region          118..373
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..376
                     /gene="yellow-g"
                     /coded_by="XM_034332908.2:700..1830"
                     /db_xref="GeneID:117608189"
ORIGIN      
        1 mkrlcitlcl mvlvkdsllt yttqdvsqsl vlsgqslnwp cqstkniyet sgryitrnvi
       61 atraqvykne ailalprykp gvpftlgvls lkssdcapki tpfpcwaiqe egncqalqsa
      121 vdialdmqgi lwvldvgivn tleqpvrrcp pkvvgvnans gkvvkvidls sladtssrlq
      181 ymavdyaadg qvyvyvsdag traiivynvt adsgyrvvlp savtsgmdkp dalylalirk
      241 scgtqvlyft ylgssrmfai kasnlrtgna ngsivdiggk knkivllgtd ngsaiffrik
      301 gdsnvymwns etpfvsdnfl lvqkagdcrl ptgvipgykg lmwviesnfq dyidnavscs
      361 gasvslhplv nsceey
//
LOCUS       XP_034188813             350 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminyl-peptide cyclotransferase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188813
VERSION     XP_034188813.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332922.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188813.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="glutaminyl-peptide cyclotransferase isoform X1"
                     /calculated_mol_wt=40664
     Region          44..345
                     /region_name="M28_QC_like"
                     /note="M28 Zn-peptidase glutaminyl cyclase; cd03880"
                     /db_xref="CDD:349876"
     Site            order(147,184..186,190,195..196,232..233,290..291,308,312,
                     316..317)
                     /site_type="active"
                     /db_xref="CDD:349876"
     CDS             1..350
                     /gene="LOC117608204"
                     /coded_by="XM_034332922.2:91..1143"
                     /db_xref="GeneID:117608204"
ORIGIN      
        1 mmfdfllqif lifvgrfitd asistetgfr nqkyyhtpvt lsrsqileis elsnvnhmdq
       61 ilsnictvri vgtpehtrvk nyikesmrnl nwtvetdifd ehtptfgtlq feniiaklnp
      121 nakrylalac hydskytrer nfvgatdsav pcaqminlak vmqnhlnsik hndislmfif
      181 fdgeeafkew gpkdsiygar hlakiwhnny ttykedenis eldkldvlvl ldligapdpt
      241 fynyfsntek wysllmaiet nlaalkefes ysygqpaqry fqpysveahi eddhipflqr
      301 dipilhlipy pfpkvwhkps dhcdnidikt teninkilri fvasylhvgk
//
LOCUS       XP_034188821             293 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminyl-peptide cyclotransferase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188821
VERSION     XP_034188821.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332930.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="glutaminyl-peptide cyclotransferase isoform X2"
                     /calculated_mol_wt=34012
     Region          2..288
                     /region_name="M28_QC_like"
                     /note="M28 Zn-peptidase glutaminyl cyclase; cd03880"
                     /db_xref="CDD:349876"
     Site            order(90,127..129,133,138..139,175..176,233..234,251,255,
                     259..260)
                     /site_type="active"
                     /db_xref="CDD:349876"
     CDS             1..293
                     /gene="LOC117608204"
                     /coded_by="XM_034332930.2:236..1117"
                     /db_xref="GeneID:117608204"
ORIGIN      
        1 mdqilsnict vrivgtpeht rvknyikesm rnlnwtvetd ifdehtptfg tlqfeniiak
       61 lnpnakryla lachydskyt rernfvgatd savpcaqmin lakvmqnhln sikhndislm
      121 fiffdgeeaf kewgpkdsiy garhlakiwh nnyttykede niseldkldv lvlldligap
      181 dptfynyfsn tekwysllma ietnlaalke fesysygqpa qryfqpysve ahieddhipf
      241 lqrdipilhl ipypfpkvwh kpsdhcdnid iktteninki lrifvasylh vgk
//
LOCUS       XP_034188830             293 aa            linear   INV 03-SEP-2025
DEFINITION  glutaminyl-peptide cyclotransferase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188830
VERSION     XP_034188830.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332939.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="glutaminyl-peptide cyclotransferase isoform X2"
                     /calculated_mol_wt=34012
     Region          2..288
                     /region_name="M28_QC_like"
                     /note="M28 Zn-peptidase glutaminyl cyclase; cd03880"
                     /db_xref="CDD:349876"
     Site            order(90,127..129,133,138..139,175..176,233..234,251,255,
                     259..260)
                     /site_type="active"
                     /db_xref="CDD:349876"
     CDS             1..293
                     /gene="LOC117608204"
                     /coded_by="XM_034332939.2:215..1096"
                     /db_xref="GeneID:117608204"
ORIGIN      
        1 mdqilsnict vrivgtpeht rvknyikesm rnlnwtvetd ifdehtptfg tlqfeniiak
       61 lnpnakryla lachydskyt rernfvgatd savpcaqmin lakvmqnhln sikhndislm
      121 fiffdgeeaf kewgpkdsiy garhlakiwh nnyttykede niseldkldv lvlldligap
      181 dptfynyfsn tekwysllma ietnlaalke fesysygqpa qryfqpysve ahieddhipf
      241 lqrdipilhl ipypfpkvwh kpsdhcdnid iktteninki lrifvasylh vgk
//
LOCUS       XP_034188861             323 aa            linear   INV 03-SEP-2025
DEFINITION  nudC domain-containing protein 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188861
VERSION     XP_034188861.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332970.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..323
                     /product="nudC domain-containing protein 3 isoform X1"
                     /calculated_mol_wt=37278
     Region          5..62
                     /region_name="Nudc_N"
                     /note="N-terminal conserved domain of Nudc; pfam14050"
                     /db_xref="CDD:464073"
     Region          139..253
                     /region_name="alpha-crystallin domain (ACD) found in
                     alpha-crystallin-type small heat shock proteins, and a
                     similar domain found in p23 (a cochaperone for Hsp90) and
                     in other p23-like proteins."
                     /note="The alpha-crystallin-Hsps_p23-like superfamily
                     includes the alpha-crystallin domain (ACD) of
                     alpha-crystallin-type small heat shock proteins (sHsps)
                     and a similar domain found in p23-like proteins. sHsps are
                     small stress induced proteins with monomeric...; cl00175"
                     /db_xref="CDD:469641"
     CDS             1..323
                     /gene="LOC117608217"
                     /coded_by="XM_034332970.2:416..1387"
                     /db_xref="GeneID:117608217"
ORIGIN      
        1 mnsthdqafl qilqeeknit nfldaffgfl yrctdfyves gpdqklgfpp gtieklvlhs
       61 lrkwkniskf asgtdppntq eviiqsndkd qdesmtieee ivvpqvdhev eietskenqi
      121 igiptqller drssdsynga irenytwsqt vsdidvlvql psciktakdl rvhldskeik
      181 ieartsrveq nqeveecryf vwttifrgel sfkvrkdesv wsivpgqyis ihlekaserw
      241 weallvgepk idltkidcsr nldemgseeq mkvqelmwnh qqkllgkpts eqikmekvlr
      301 kawdakgspf egtpydpsii nfn
//
LOCUS       XP_076546460             298 aa            linear   INV 03-SEP-2025
DEFINITION  nudC domain-containing protein 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546460
VERSION     XP_076546460.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690345.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="nudC domain-containing protein 3 isoform X2"
                     /calculated_mol_wt=34290
     Region          <7..37
                     /region_name="Nudc_N"
                     /note="N-terminal conserved domain of Nudc; pfam14050"
                     /db_xref="CDD:464073"
     Region          114..228
                     /region_name="alpha-crystallin-Hsps_p23-like"
                     /note="alpha-crystallin domain (ACD) found in
                     alpha-crystallin-type small heat shock proteins, and a
                     similar domain found in p23 (a cochaperone for Hsp90) and
                     in other p23-like proteins; cl00175"
                     /db_xref="CDD:469641"
     CDS             1..298
                     /gene="LOC117608217"
                     /coded_by="XM_076690345.1:16..912"
                     /db_xref="GeneID:117608217"
ORIGIN      
        1 mhsldfctdd fyvesgpdqk lgfppgtiek lvlhslrkwk niskfasgtd ppntqeviiq
       61 sndkdqdesm tieeeivvpq vdheveiets kenqiigipt qllerdrssd syngaireny
      121 twsqtvsdid vlvqlpscik takdlrvhld skeikieart srveqnqeve ecryfvwtti
      181 frgelsfkvr kdesvwsivp gqyisihlek aserwweall vgepkidltk idcsrnldem
      241 gseeqmkvqe lmwnhqqkll gkptseqikm ekvlrkawda kgspfegtpy dpsiinfn
//
LOCUS       XP_076546461             309 aa            linear   INV 03-SEP-2025
DEFINITION  UPF0764 protein C16orf89 homolog [Osmia lignaria lignaria].
ACCESSION   XP_076546461
VERSION     XP_076546461.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690346.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..309
                     /product="UPF0764 protein C16orf89 homolog"
                     /calculated_mol_wt=35707
     Region          56..307
                     /region_name="DUF4735"
                     /note="Domain of unknown function (DUF4735); pfam15882"
                     /db_xref="CDD:464922"
     CDS             1..309
                     /gene="LOC117608216"
                     /coded_by="XM_076690346.1:23..952"
                     /db_xref="GeneID:117608216"
ORIGIN      
        1 mdrvrrflsf wlillfsdyl rlvdgkfvae dfernlials kvinyihhrp hqmnadvtls
       61 itiveanian tllhknaqyl gteyqkmlmt ilkmsdstrr yllnnivpvn rdvqllhetl
      121 nnpvlwmrpi swkegvlkrr rvssslndqd irelimqgtp keeesdrcls diirnksnlg
      181 ytipelcvdi lmgpgtsrgy plthrllivq vakamncdqa lpvssseltq yycskilqdl
      241 tdiqavgfpy htpdlmmeqv llcgmegfle ftddhydglv rnwshpsgcy ssfgyvlkrk
      301 rsfsrirkq
//
LOCUS       XP_034188877             288 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608218 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188877
VERSION     XP_034188877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="uncharacterized protein LOC117608218 isoform X1"
                     /calculated_mol_wt=32551
     Region          16..231
                     /region_name="Claudin_2"
                     /note="PMP-22/EMP/MP20/Claudin tight junction; pfam13903"
                     /db_xref="CDD:372799"
     CDS             1..288
                     /gene="LOC117608218"
                     /coded_by="XM_034332986.2:397..1263"
                     /db_xref="GeneID:117608218"
ORIGIN      
        1 mpcsavtlsl atitgiiatg llaiafstdn wlytevkraq iqqyaakhae qshlveqmnt
       61 kyyyytrtqg lfricypker pptvqtylsp vethcmniny fipdeenltr gfsddamtrl
      121 hmgrsvialf ivgfvaifta fwtgvvgcwk rspgnitata ilmllaclls asgmglwhgv
      181 eyyekekivg eeyyqqwsnv lkdnslisyd wsyvvawvgv gwslvsailf saaaiclrge
      241 rmreeamnmq ylmpvypqkq qyayagypap aaypgpyyhg sqygpyny
//
LOCUS       XP_034188887             288 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608218 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188887
VERSION     XP_034188887.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034332996.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="uncharacterized protein LOC117608218 isoform X2"
                     /calculated_mol_wt=32718
     Region          16..231
                     /region_name="Claudin_2"
                     /note="PMP-22/EMP/MP20/Claudin tight junction; pfam13903"
                     /db_xref="CDD:372799"
     CDS             1..288
                     /gene="LOC117608218"
                     /coded_by="XM_034332996.2:399..1265"
                     /db_xref="GeneID:117608218"
ORIGIN      
        1 mpcsavtlsl atitgiiatg llaiafstdn wlytevkraq iqqyaakhae qshlveqmnt
       61 kyyyytrtqg lfricypker pptvqtylsp vethcmniny fipdeenltr gfsddamtrl
      121 hmgrsvialf ivgfvaifta fwtgvvgcwk rspgnitata ilmllaclls asgmglwhgv
      181 eyyekekivg eeyyqqwsnv lrdntvqwyg wsfylawlgv aacfgtmllf liaasclrre
      241 hareqaqnvq yimpvypqkq qyayagypap aaypgpyyhg sqygpyny
//
LOCUS       XP_034188898             231 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608218 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188898
VERSION     XP_034188898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333007.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..231
                     /product="uncharacterized protein LOC117608218 isoform X3"
                     /calculated_mol_wt=26364
     Region          4..174
                     /region_name="Claudin_2"
                     /note="PMP-22/EMP/MP20/Claudin tight junction; pfam13903"
                     /db_xref="CDD:372799"
     CDS             1..231
                     /gene="LOC117608218"
                     /coded_by="XM_034333007.2:434..1129"
                     /db_xref="GeneID:117608218"
ORIGIN      
        1 mntkyyyytr tqglfricyp kerpptvqty lspvethcmn inyfipdeen ltrgfsddam
       61 trlhmgrsvi alfivgfvai ftafwtgvvg cwkrspgnit atailmllac llsasgmglw
      121 hgveyyekek ivgeeyyqqw snvlkdnsli sydwsyvvaw vgvgwslvsa ilfsaaaicl
      181 rgermreeam nmqylmpvyp qkqqyayagy papaaypgpy yhgsqygpyn y
//
LOCUS       XP_034188912             260 aa            linear   INV 03-SEP-2025
DEFINITION  L-xylulose reductase [Osmia lignaria lignaria].
ACCESSION   XP_034188912
VERSION     XP_034188912.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188912.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="L-xylulose reductase"
                     /calculated_mol_wt=28154
     Region          1..243
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(14,16..17,19,38..40,83..85,134..136,149,153,
                     179..182)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187609"
     Site            order(107,136,149,153)
                     /site_type="active"
                     /db_xref="CDD:187609"
     CDS             1..260
                     /gene="LOC117608225"
                     /coded_by="XM_034333021.2:151..933"
                     /db_xref="GeneID:117608225"
ORIGIN      
        1 mninfegkrv lvtgagqgig rdialrlsqy kaqvialskt kknleklcae dpriqsvcvd
       61 lrdwdatkra vqsvlpidll vnnagvahlk pfldatpedf dsifavnvka imnvsqvvar
      121 nmierkvpgn ivnissqasq aalkdhavyc sskgavdmls ktmalelgpy nirvntvnpt
      181 vimtemgklg wsdpdkartm lskiplgrfg evsevvdavv yllsnrssmi ngvtlpvdgg
      241 flairdslit depniqdtmv
//
LOCUS       XP_034188925             167 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608237 [Osmia lignaria lignaria].
ACCESSION   XP_034188925
VERSION     XP_034188925.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..167
                     /product="uncharacterized protein LOC117608237"
                     /calculated_mol_wt=18955
     CDS             1..167
                     /gene="LOC117608237"
                     /coded_by="XM_034333034.2:144..647"
                     /db_xref="GeneID:117608237"
ORIGIN      
        1 mqltsccrcy slkagtlftg icgiilsiis lvliftlnve wktilidvld knivriifai
       61 nlcmtilist llivgvlkkn tfmmipwvvl giilavgllv svlytsimff vnhevingvl
      121 wlvlglvavv vytylwfvvy syfqqlrydk vnnrmgpygr pysyrrp
//
LOCUS       XP_034188936             167 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608237 [Osmia lignaria lignaria].
ACCESSION   XP_034188936
VERSION     XP_034188936.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333045.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..167
                     /product="uncharacterized protein LOC117608237"
                     /calculated_mol_wt=18955
     CDS             1..167
                     /gene="LOC117608237"
                     /coded_by="XM_034333045.2:137..640"
                     /db_xref="GeneID:117608237"
ORIGIN      
        1 mqltsccrcy slkagtlftg icgiilsiis lvliftlnve wktilidvld knivriifai
       61 nlcmtilist llivgvlkkn tfmmipwvvl giilavgllv svlytsimff vnhevingvl
      121 wlvlglvavv vytylwfvvy syfqqlrydk vnnrmgpygr pysyrrp
//
LOCUS       XP_034188946             167 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608237 [Osmia lignaria lignaria].
ACCESSION   XP_034188946
VERSION     XP_034188946.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333055.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..167
                     /product="uncharacterized protein LOC117608237"
                     /calculated_mol_wt=18955
     CDS             1..167
                     /gene="LOC117608237"
                     /coded_by="XM_034333055.1:21..524"
                     /db_xref="GeneID:117608237"
ORIGIN      
        1 mqltsccrcy slkagtlftg icgiilsiis lvliftlnve wktilidvld knivriifai
       61 nlcmtilist llivgvlkkn tfmmipwvvl giilavgllv svlytsimff vnhevingvl
      121 wlvlglvavv vytylwfvvy syfqqlrydk vnnrmgpygr pysyrrp
//
LOCUS       XP_034188960             137 aa            linear   INV 03-SEP-2025
DEFINITION  prefoldin subunit 4 [Osmia lignaria lignaria].
ACCESSION   XP_034188960
VERSION     XP_034188960.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333069.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..137
                     /product="prefoldin subunit 4"
                     /calculated_mol_wt=16060
     Region          23..125
                     /region_name="Prefoldin_4"
                     /note="prefoldin subunit 4; cd23165"
                     /db_xref="CDD:467481"
     Site            order(23,25..28,31..32,35,102,105..106,108..109,112..113,
                     115..117,119..121,123..125)
                     /site_type="other"
                     /note="prefoldin/TRiC interface [polypeptide binding]"
                     /db_xref="CDD:467481"
     Region          23..64
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467481"
     Site            order(60..61,65,69,71..80,86,90)
                     /site_type="other"
                     /note="prefoldin oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467481"
     Region          84..125
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:467481"
     CDS             1..137
                     /gene="Pfdn4"
                     /coded_by="XM_034333069.2:128..541"
                     /db_xref="GeneID:117608248"
ORIGIN      
        1 msarkntqag fqpdsdiyvt yedqqkinkf arqnakmedl keelkikqne lknledacde
       61 vvlfddgaki pyyigevfiy edmektheyl eqlmekkqae idnleskcrd lknmmselkt
      121 qlyakfgnri nleaeed
//
LOCUS       XP_034188974             116 aa            linear   INV 03-SEP-2025
DEFINITION  protein C10 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034188974
VERSION     XP_034188974.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333083.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="protein C10 isoform X1"
                     /calculated_mol_wt=12764
     Region          7..109
                     /region_name="P_C10"
                     /note="Protein C10; pfam14974"
                     /db_xref="CDD:464416"
     CDS             1..116
                     /gene="LOC117608251"
                     /coded_by="XM_034333083.2:238..588"
                     /db_xref="GeneID:117608251"
ORIGIN      
        1 mttipaftpd iakavltdvl talnapeniq klaeakensg nemlkmmqfv fpiivqiqmd
       61 viknygfpeg regtvqfaql lraleredpe iaqlhsqvrs yflppvtiss steasl
//
LOCUS       XP_034188980             113 aa            linear   INV 03-SEP-2025
DEFINITION  protein C10 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034188980
VERSION     XP_034188980.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333089.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..113
                     /product="protein C10 isoform X2"
                     /calculated_mol_wt=12421
     Region          7..106
                     /region_name="P_C10"
                     /note="Protein C10; pfam14974"
                     /db_xref="CDD:464416"
     CDS             1..113
                     /gene="LOC117608251"
                     /coded_by="XM_034333089.2:259..600"
                     /db_xref="GeneID:117608251"
ORIGIN      
        1 mttipaftpd iakavltdvl talnapeniq klaeakensg nemlkmmqfv fpiivqiqmd
       61 viknygfpeg tvqfaqllra leredpeiaq lhsqvrsyfl ppvtissste asl
//
LOCUS       XP_034188996             102 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608259 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034188996
VERSION     XP_034188996.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333105.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034188996.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="uncharacterized protein LOC117608259 isoform X1"
                     /calculated_mol_wt=11349
     CDS             1..102
                     /gene="LOC117608259"
                     /coded_by="XM_034333105.2:1586..1894"
                     /db_xref="GeneID:117608259"
ORIGIN      
        1 mtsnfvcllv liaslnfatr issrtvesgd lmnsiqspvm mthsypnmik tmqekldegg
       61 erfcqieyqv trkrsgkcmq isngitgcis geyidpfhpd cf
//
LOCUS       XP_034189006             102 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608259 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189006
VERSION     XP_034189006.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333115.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189006.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="uncharacterized protein LOC117608259 isoform X1"
                     /calculated_mol_wt=11349
     CDS             1..102
                     /gene="LOC117608259"
                     /coded_by="XM_034333115.2:1522..1830"
                     /db_xref="GeneID:117608259"
ORIGIN      
        1 mtsnfvcllv liaslnfatr issrtvesgd lmnsiqspvm mthsypnmik tmqekldegg
       61 erfcqieyqv trkrsgkcmq isngitgcis geyidpfhpd cf
//
LOCUS       XP_034189016              71 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608259 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189016
VERSION     XP_034189016.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333125.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189016.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..71
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..71
                     /product="uncharacterized protein LOC117608259 isoform X2"
                     /calculated_mol_wt=8008
     CDS             1..71
                     /gene="LOC117608259"
                     /coded_by="XM_034333125.2:219..434"
                     /db_xref="GeneID:117608259"
ORIGIN      
        1 mnsiqspvmm thsypnmikt mqekldegge rfcqieyqvt rkrsgkcmqi sngitgcisg
       61 eyidpfhpdc f
//
LOCUS       XP_034189025              71 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608259 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189025
VERSION     XP_034189025.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333134.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189025.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..71
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..71
                     /product="uncharacterized protein LOC117608259 isoform X2"
                     /calculated_mol_wt=8008
     CDS             1..71
                     /gene="LOC117608259"
                     /coded_by="XM_034333134.2:372..587"
                     /db_xref="GeneID:117608259"
ORIGIN      
        1 mnsiqspvmm thsypnmikt mqekldegge rfcqieyqvt rkrsgkcmqi sngitgcisg
       61 eyidpfhpdc f
//
LOCUS       XP_034189034              71 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608259 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189034
VERSION     XP_034189034.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034333143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189034.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..71
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..71
                     /product="uncharacterized protein LOC117608259 isoform X2"
                     /calculated_mol_wt=8008
     CDS             1..71
                     /gene="LOC117608259"
                     /coded_by="XM_034333143.2:572..787"
                     /db_xref="GeneID:117608259"
ORIGIN      
        1 mnsiqspvmm thsypnmikt mqekldegge rfcqieyqvt rkrsgkcmqi sngitgcisg
       61 eyidpfhpdc f
//
LOCUS       XP_076546462             644 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor coactivator protein neosin [Osmia lignaria
            lignaria].
ACCESSION   XP_076546462
VERSION     XP_076546462.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690347.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..644
                     /product="nuclear receptor coactivator protein neosin"
                     /calculated_mol_wt=69969
     Region          14..80
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          521..>642
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..644
                     /gene="Neos"
                     /coded_by="XM_076690347.1:288..2222"
                     /db_xref="GeneID:117608277"
ORIGIN      
        1 msvrllkdpa tvssrifvgh lqtddmtkhe leehfskygt vvgslinrgf gfvqfeeeqs
       61 aqkaiqnedg amfkgrridv rpakkdnqss gggggagklg gpnnqfnsnn nhfnpgndsf
      121 nsgnqsyggn snfnpnqsfs cdpqlndsqk gnaiqnrsgg ndqfndsnqn rdnandqfan
      181 qnrnsgndqf nmmqnkgnnq fnpnnqnrsg tdqfpqnrgg nqfgpggnqn rpggnqnrna
      241 nnqnnhsqrg gggggmnsgg ggrargrgak nrnknrdnsv gnnfrdrspi nrggrlddkg
      301 grdwdhkqgd rlrgsnfgrd sfndtnnrye dfknsgprdm smsfnntnas nvvtekndce
      361 iivvnkalte yaesiemrlk kigltvdllf pnedvllsrv lgniasrgcl yavvvtpinq
      421 ehhsltlnil hglpqehrnm lvddainlis rdfanykagg rsvplntpfa nerhpdaiqv
      481 llnmladnhq ltvlqydrvi kyleikreeq vqvelgdvkd lptatttitv sdpkqaelqs
      541 rilnilnsnk tsssaptpsp vpaptstpap vppatwgaas tattasttts tttgvspspl
      601 lndptvqkal dsllqgnllk sigdqqpatt ttplfaafsn igrf
//
LOCUS       XP_034183776            2314 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605978 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183776
VERSION     XP_034183776.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327885.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183776.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2314
                     /product="uncharacterized protein LOC117605978 isoform X1"
                     /calculated_mol_wt=258969
     Region          405..501
                     /region_name="Bromo_gcn5_like"
                     /note="Bromodomain; Gcn5_like subfamily. Gcn5p is a
                     histone acetyltransferase (HAT) which mediates acetylation
                     of histones at lysine residues; such acetylation is
                     generally correlated with the activation of transcription.
                     Bromodomains are 110 amino acid long...; cd05509"
                     /db_xref="CDD:99941"
     Site            order(426,431,434,473,477,483)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99941"
     Region          632..>948
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1207..1622
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2314
                     /gene="LOC117605978"
                     /coded_by="XM_034327885.2:697..7641"
                     /db_xref="GeneID:117605978"
ORIGIN      
        1 mfptfigild iqswwevpsi ahfcslfraa fnlldfdied leealltdgg tegrlvqeli
       61 vrllegclpn dtrndistfn yqmflrrlfr kkcqeykcen pfntdvdfel lplrqkveil
      121 ralcdfrlda edveqslsnl dsdslrvepl ghdrknsayw yfygtrlyre dyidtsnsis
      181 ykqknkprdk krkrrrnrva keeeeeeekk edsliqgegk esvwqvvcft qqdwsrlvek
      241 frdseydter klyrtlsedf mpeipklfdl kekqqrrkll qrnssrvlrs hepttqmetv
      301 mvrskiktkt nkgnkkgtqn sknvyvkeet spplssppvq kkgrqtnnsl asavgqivih
      361 trdevegtek kkgvgsnsdg nyvasnygyk fgysfgieee errvgmhkvl eslkdhvdaw
      421 pfidpvdeey apryysvvrk pmdlstmeek lenglyknln efkrdfrliv dncrqyngsd
      481 neytemafnl keafdkavnr ylesetssde dasspksclt tpaspscpsr vssphqnkkr
      541 skkstkksks ykseskgksk asdkndeeek rgknyklpkk krgkkkskra kdeesvneee
      601 qeeqesedam sessiitskr rkhldvnnll lktkklsske sdelkkidkk mskerhqqkk
      661 eaesmkpmke skenkkrked feedyeplvi aksksrkiek keleeteift dnakknkvkk
      721 neshenevms enkdkiqhik vkknrkkeka glkalksdek sekteknhvk dkekkskqkn
      781 delkngelsn eldskdvdle sdldskemha skkipafigd kdiesldglk dkiserrree
      841 klknekekpk ktsknelhnm yskkvpsngs tfndsdkasa krpklkkgmk eekvpitedt
      901 vktqdqemne tkkvkvaqtk stkgfgkees siqalnqate qtlhdinkwl ddaprlsefs
      961 sgsdspvfhs svefgrsgpk veaprkrpss ikifgphgps rpkkiqrtid rlqpgkskgn
     1021 lllkkplnlp nvgsnettvh pltgdndqtn knadeepkls lgtvlknvds iqlickslvs
     1081 spnpnfsndd eeedhntvpa ipvsslkeek sssgatstqa paateeskdn qssvketqkp
     1141 kaatpnlsaw fkafgapksk kkdeesedst akkdgelqev fygrqrrmst ggssvsesvs
     1201 sfsqesppgr sgrspqgqpi masvepqirg agfyqdalst gsspynspyy atpprysaql
     1261 pptpspqnhp lspaypsssf eqapplysqi paqqphqtfq kspqensgev yhqlsptfpq
     1321 rspqtnnfpp nptpasepfn qpvqsttpnp sqsatysqhs pqpvqqvypq pspqpppsny
     1381 sqpspqqspt pkysqlspqq naanysqpsp qasnysqasp qqphspysqn spqapppnys
     1441 qpspqpqpsp yaqspqqsag ysqlspqpps nysqpspqpp anysqpspqp panysqpspq
     1501 ppsnysqpsp qpppvypqqs qpanfshpsp qthsvgftqs spqpltaysq pspqprnysq
     1561 pspqqiqpfp qhspqpppsy sqpspqnapy sqpspqqsak ysqpspqqpt nyshsihspq
     1621 tpqnysqlsp qqappsnysq pspspqqsrn ysqpspspqq srnysqpsps sqqshnysqp
     1681 spspqtrsys qpspqqksac psqtsqqpsn ysqpspqqst nyalqqqstk tteeypqnas
     1741 tpsnyshgyk qnhsyiqspt pssinetehr adymksssge ksssieqqrn ftvppeasln
     1801 lnanhqiyqs pnqypptfgn nypnidlqrs vsrhggqeqp qqqqpqqqqq qqqqqqqqqa
     1861 pqerpsftdl neslnaqkya qqrsflgktg stgeqlssqd qsqilafqqn ltaheslyps
     1921 sfqpsgypmp nsrpmypsph yfdtssktan sgtansssgn lppvkkrvyn essteasrgl
     1981 tqeaasrtgq eqfgfdpima lpqpeavsas qfdatfvgnl adsvatnpaa yarlglglvg
     2041 rtskeqqqll tiprppptka ehlayarspa tgaaeldlnl lqslqtasak nsqgilsmsr
     2101 rggdtssgst sapaktkksr kskqqqqeqq nianvsstvn tepqantgip gfpqytgtsa
     2161 dsiglkntam vppagsafnf aastntttss pfydkeataa aaafafldef rnpnsyysma
     2221 lrqqqqqqqq pqvppvsdat qqacnklsnq pprnypphpf lhsaqrsaay gppvsayvtp
     2281 hgpnltvdpt ayqqyihsly alqppphhhr pswl
//
LOCUS       XP_034183785            2278 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605978 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183785
VERSION     XP_034183785.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183785.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2278
                     /product="uncharacterized protein LOC117605978 isoform X2"
                     /calculated_mol_wt=254797
     Region          369..465
                     /region_name="Bromo_gcn5_like"
                     /note="Bromodomain; Gcn5_like subfamily. Gcn5p is a
                     histone acetyltransferase (HAT) which mediates acetylation
                     of histones at lysine residues; such acetylation is
                     generally correlated with the activation of transcription.
                     Bromodomains are 110 amino acid long...; cd05509"
                     /db_xref="CDD:99941"
     Site            order(390,395,398,437,441,447)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99941"
     Region          596..>912
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1171..1586
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2278
                     /gene="LOC117605978"
                     /coded_by="XM_034327894.2:129..6965"
                     /db_xref="GeneID:117605978"
ORIGIN      
        1 mrldleeall tdggtegrlv qelivrlleg clpndtrndi stfnyqmflr rlfrkkcqey
       61 kcenpfntdv dfellplrqk veilralcdf rldaedveqs lsnldsdslr veplghdrkn
      121 saywyfygtr lyredyidts nsisykqknk prdkkrkrrr nrvakeeeee eekkedsliq
      181 gegkesvwqv vcftqqdwsr lvekfrdsey dterklyrtl sedfmpeipk lfdlkekqqr
      241 rkllqrnssr vlrshepttq metvmvrski ktktnkgnkk gtqnsknvyv keetspplss
      301 ppvqkkgrqt nnslasavgq ivihtrdeve gtekkkgvgs nsdgnyvasn ygykfgysfg
      361 ieeeerrvgm hkvleslkdh vdawpfidpv deeyapryys vvrkpmdlst meeklengly
      421 knlnefkrdf rlivdncrqy ngsdneytem afnlkeafdk avnryleset ssdedasspk
      481 sclttpasps cpsrvssphq nkkrskkstk ksksyksesk gkskasdknd eeekrgknyk
      541 lpkkkrgkkk skrakdeesv neeeqeeqes edamsessii tskrrkhldv nnlllktkkl
      601 sskesdelkk idkkmskerh qqkkeaesmk pmkeskenkk rkedfeedye plviaksksr
      661 kiekkeleet eiftdnakkn kvkkneshen evmsenkdki qhikvkknrk kekaglkalk
      721 sdeksektek nhvkdkekks kqkndelkng elsneldskd vdlesdldsk emhaskkipa
      781 figdkdiesl dglkdkiser rreeklknek ekpkktskne lhnmyskkvp sngstfndsd
      841 kasakrpklk kgmkeekvpi tedtvktqdq emnetkkvkv aqtkstkgfg keessiqaln
      901 qateqtlhdi nkwlddaprl sefssgsdsp vfhssvefgr sgpkveaprk rpssikifgp
      961 hgpsrpkkiq rtidrlqpgk skgnlllkkp lnlpnvgsne ttvhpltgdn dqtnknadee
     1021 pklslgtvlk nvdsiqlick slvsspnpnf snddeeedhn tvpaipvssl keeksssgat
     1081 stqapaatee skdnqssvke tqkpkaatpn lsawfkafga pkskkkdees edstakkdge
     1141 lqevfygrqr rmstggssvs esvssfsqes ppgrsgrspq gqpimasvep qirgagfyqd
     1201 alstgsspyn spyyatppry saqlpptpsp qnhplspayp sssfeqappl ysqipaqqph
     1261 qtfqkspqen sgevyhqlsp tfpqrspqtn nfppnptpas epfnqpvqst tpnpsqsaty
     1321 sqhspqpvqq vypqpspqpp psnysqpspq qsptpkysql spqqnaanys qpspqasnys
     1381 qaspqqphsp ysqnspqapp pnysqpspqp qpspyaqspq qsagysqlsp qppsnysqps
     1441 pqppanysqp spqppanysq pspqppsnys qpspqpppvy pqqsqpanfs hpspqthsvg
     1501 ftqsspqplt aysqpspqpr nysqpspqqi qpfpqhspqp ppsysqpspq napysqpspq
     1561 qsakysqpsp qqptnyshsi hspqtpqnys qlspqqapps nysqpspspq qsrnysqpsp
     1621 spqqsrnysq pspssqqshn ysqpspspqt rsysqpspqq ksacpsqtsq qpsnysqpsp
     1681 qqstnyalqq qstktteeyp qnastpsnys hgykqnhsyi qsptpssine tehradymks
     1741 ssgeksssie qqrnftvppe aslnlnanhq iyqspnqypp tfgnnypnid lqrsvsrhgg
     1801 qeqpqqqqpq qqqqqqqqqq qqqapqerps ftdlneslna qkyaqqrsfl gktgstgeql
     1861 ssqdqsqila fqqnltahes lypssfqpsg ypmpnsrpmy psphyfdtss ktansgtans
     1921 ssgnlppvkk rvynesstea srgltqeaas rtgqeqfgfd pimalpqpea vsasqfdatf
     1981 vgnladsvat npaayarlgl glvgrtskeq qqlltiprpp ptkaehlaya rspatgaael
     2041 dlnllqslqt asaknsqgil smsrrggdts sgstsapakt kksrkskqqq qeqqnianvs
     2101 stvntepqan tgipgfpqyt gtsadsiglk ntamvppags afnfaastnt ttsspfydke
     2161 ataaaaafaf ldefrnpnsy ysmalrqqqq qqqqpqvppv sdatqqacnk lsnqpprnyp
     2221 phpflhsaqr saaygppvsa yvtphgpnlt vdptayqqyi hslyalqppp hhhrpswl
//
LOCUS       XP_034183793            2232 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605978 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183793
VERSION     XP_034183793.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327902.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183793.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2232
                     /product="uncharacterized protein LOC117605978 isoform X3"
                     /calculated_mol_wt=249592
     Region          323..419
                     /region_name="Bromo_gcn5_like"
                     /note="Bromodomain; Gcn5_like subfamily. Gcn5p is a
                     histone acetyltransferase (HAT) which mediates acetylation
                     of histones at lysine residues; such acetylation is
                     generally correlated with the activation of transcription.
                     Bromodomains are 110 amino acid long...; cd05509"
                     /db_xref="CDD:99941"
     Site            order(344,349,352,391,395,401)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99941"
     Region          550..>866
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1125..1540
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2232
                     /gene="LOC117605978"
                     /coded_by="XM_034327902.2:854..7552"
                     /db_xref="GeneID:117605978"
ORIGIN      
        1 mflrrlfrkk cqeykcenpf ntdvdfellp lrqkveilra lcdfrldaed veqslsnlds
       61 dslrveplgh drknsaywyf ygtrlyredy idtsnsisyk qknkprdkkr krrrnrvake
      121 eeeeeekked sliqgegkes vwqvvcftqq dwsrlvekfr dseydterkl yrtlsedfmp
      181 eipklfdlke kqqrrkllqr nssrvlrshe pttqmetvmv rskiktktnk gnkkgtqnsk
      241 nvyvkeetsp plssppvqkk grqtnnslas avgqivihtr devegtekkk gvgsnsdgny
      301 vasnygykfg ysfgieeeer rvgmhkvles lkdhvdawpf idpvdeeyap ryysvvrkpm
      361 dlstmeekle nglyknlnef krdfrlivdn crqyngsdne ytemafnlke afdkavnryl
      421 esetssdeda sspksclttp aspscpsrvs sphqnkkrsk kstkksksyk seskgkskas
      481 dkndeeekrg knyklpkkkr gkkkskrakd eesvneeeqe eqesedamse ssiitskrrk
      541 hldvnnlllk tkklsskesd elkkidkkms kerhqqkkea esmkpmkesk enkkrkedfe
      601 edyeplviak sksrkiekke leeteiftdn akknkvkkne shenevmsen kdkiqhikvk
      661 knrkkekagl kalksdekse kteknhvkdk ekkskqknde lkngelsnel dskdvdlesd
      721 ldskemhask kipafigdkd iesldglkdk iserrreekl knekekpkkt sknelhnmys
      781 kkvpsngstf ndsdkasakr pklkkgmkee kvpitedtvk tqdqemnetk kvkvaqtkst
      841 kgfgkeessi qalnqateqt lhdinkwldd aprlsefssg sdspvfhssv efgrsgpkve
      901 aprkrpssik ifgphgpsrp kkiqrtidrl qpgkskgnll lkkplnlpnv gsnettvhpl
      961 tgdndqtnkn adeepklslg tvlknvdsiq lickslvssp npnfsnddee edhntvpaip
     1021 vsslkeekss sgatstqapa ateeskdnqs svketqkpka atpnlsawfk afgapkskkk
     1081 deesedstak kdgelqevfy grqrrmstgg ssvsesvssf sqesppgrsg rspqgqpima
     1141 svepqirgag fyqdalstgs spynspyyat pprysaqlpp tpspqnhpls paypsssfeq
     1201 applysqipa qqphqtfqks pqensgevyh qlsptfpqrs pqtnnfppnp tpasepfnqp
     1261 vqsttpnpsq satysqhspq pvqqvypqps pqpppsnysq pspqqsptpk ysqlspqqna
     1321 anysqpspqa snysqaspqq phspysqnsp qapppnysqp spqpqpspya qspqqsagys
     1381 qlspqppsny sqpspqppan ysqpspqppa nysqpspqpp snysqpspqp ppvypqqsqp
     1441 anfshpspqt hsvgftqssp qpltaysqps pqprnysqps pqqiqpfpqh spqpppsysq
     1501 pspqnapysq pspqqsakys qpspqqptny shsihspqtp qnysqlspqq appsnysqps
     1561 pspqqsrnys qpspspqqsr nysqpspssq qshnysqpsp spqtrsysqp spqqksacps
     1621 qtsqqpsnys qpspqqstny alqqqstktt eeypqnastp snyshgykqn hsyiqsptps
     1681 sinetehrad ymksssgeks ssieqqrnft vppeaslnln anhqiyqspn qypptfgnny
     1741 pnidlqrsvs rhggqeqpqq qqpqqqqqqq qqqqqqqapq erpsftdlne slnaqkyaqq
     1801 rsflgktgst geqlssqdqs qilafqqnlt aheslypssf qpsgypmpns rpmypsphyf
     1861 dtssktansg tansssgnlp pvkkrvynes steasrgltq eaasrtgqeq fgfdpimalp
     1921 qpeavsasqf datfvgnlad svatnpaaya rlglglvgrt skeqqqllti prppptkaeh
     1981 layarspatg aaeldlnllq slqtasakns qgilsmsrrg gdtssgstsa paktkksrks
     2041 kqqqqeqqni anvsstvnte pqantgipgf pqytgtsads iglkntamvp pagsafnfaa
     2101 stntttsspf ydkeataaaa afafldefrn pnsyysmalr qqqqqqqqpq vppvsdatqq
     2161 acnklsnqpp rnypphpflh saqrsaaygp pvsayvtphg pnltvdptay qqyihslyal
     2221 qppphhhrps wl
//
LOCUS       XP_034183803            2133 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605978 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183803
VERSION     XP_034183803.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327912.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183803.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2133
                     /product="uncharacterized protein LOC117605978 isoform X4"
                     /calculated_mol_wt=237623
     Region          224..320
                     /region_name="Bromo_gcn5_like"
                     /note="Bromodomain; Gcn5_like subfamily. Gcn5p is a
                     histone acetyltransferase (HAT) which mediates acetylation
                     of histones at lysine residues; such acetylation is
                     generally correlated with the activation of transcription.
                     Bromodomains are 110 amino acid long...; cd05509"
                     /db_xref="CDD:99941"
     Site            order(245,250,253,292,296,302)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99941"
     Region          451..>767
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1060..1441
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2133
                     /gene="LOC117605978"
                     /coded_by="XM_034327912.2:136..6537"
                     /db_xref="GeneID:117605978"
ORIGIN      
        1 miknkprdkk rkrrrnrvak eeeeeeekke dsliqgegke svwqvvcftq qdwsrlvekf
       61 rdseydterk lyrtlsedfm peipklfdlk ekqqrrkllq rnssrvlrsh epttqmetvm
      121 vrskiktktn kgnkkgtqns knvyvkeets pplssppvqk kgrqtnnsla savgqiviht
      181 rdevegtekk kgvgsnsdgn yvasnygykf gysfgieeee rrvgmhkvle slkdhvdawp
      241 fidpvdeeya pryysvvrkp mdlstmeekl englyknlne fkrdfrlivd ncrqyngsdn
      301 eytemafnlk eafdkavnry lesetssded asspkscltt paspscpsrv ssphqnkkrs
      361 kkstkksksy kseskgkska sdkndeeekr gknyklpkkk rgkkkskrak deesvneeeq
      421 eeqesedams essiitskrr khldvnnlll ktkklsskes delkkidkkm skerhqqkke
      481 aesmkpmkes kenkkrkedf eedyeplvia ksksrkiekk eleeteiftd nakknkvkkn
      541 eshenevmse nkdkiqhikv kknrkkekag lkalksdeks ekteknhvkd kekkskqknd
      601 elkngelsne ldskdvdles dldskemhas kkipafigdk diesldglkd kiserrreek
      661 lknekekpkk tsknelhnmy skkvpsngst fndsdkasak rpklkkgmke ekvpitedtv
      721 ktqdqemnet kkvkvaqtks tkgfgkeess iqalnqateq tlhdinkwld daprlsefss
      781 gsdspvfhss vefgrsgpkv eaprkrpssi kifgphgpsr pkkiqrtidr lqpgkskgnl
      841 llkkplnlpn vgsnettvhp ltgdndqtnk nadeepklsl gtvlknvdsi qlickslvss
      901 pnpnfsndde eedhntvpai pvsslkeeks ssgatstqap aateeskdnq ssvketqkpk
      961 aatpnlsawf kafgapkskk kdeesedsta kkdgelqevf ygrqrrmstg gssvsesvss
     1021 fsqesppgrs grspqgqpim asvepqirga gfyqdalstg sspynspyya tpprysaqlp
     1081 ptpspqnhpl spaypsssfe qapplysqip aqqphqtfqk spqensgevy hqlsptfpqr
     1141 spqtnnfppn ptpasepfnq pvqsttpnps qsatysqhsp qpvqqvypqp spqpppsnys
     1201 qpspqqsptp kysqlspqqn aanysqpspq asnysqaspq qphspysqns pqapppnysq
     1261 pspqpqpspy aqspqqsagy sqlspqppsn ysqpspqppa nysqpspqpp anysqpspqp
     1321 psnysqpspq pppvypqqsq panfshpspq thsvgftqss pqpltaysqp spqprnysqp
     1381 spqqiqpfpq hspqpppsys qpspqnapys qpspqqsaky sqpspqqptn yshsihspqt
     1441 pqnysqlspq qappsnysqp spspqqsrny sqpspspqqs rnysqpspss qqshnysqps
     1501 pspqtrsysq pspqqksacp sqtsqqpsny sqpspqqstn yalqqqstkt teeypqnast
     1561 psnyshgykq nhsyiqsptp ssinetehra dymksssgek sssieqqrnf tvppeaslnl
     1621 nanhqiyqsp nqypptfgnn ypnidlqrsv srhggqeqpq qqqpqqqqqq qqqqqqqqap
     1681 qerpsftdln eslnaqkyaq qrsflgktgs tgeqlssqdq sqilafqqnl taheslypss
     1741 fqpsgypmpn srpmypsphy fdtssktans gtansssgnl ppvkkrvyne ssteasrglt
     1801 qeaasrtgqe qfgfdpimal pqpeavsasq fdatfvgnla dsvatnpaay arlglglvgr
     1861 tskeqqqllt iprppptkae hlayarspat gaaeldlnll qslqtasakn sqgilsmsrr
     1921 ggdtssgsts apaktkksrk skqqqqeqqn ianvsstvnt epqantgipg fpqytgtsad
     1981 siglkntamv ppagsafnfa astntttssp fydkeataaa aafafldefr npnsyysmal
     2041 rqqqqqqqqp qvppvsdatq qacnklsnqp prnypphpfl hsaqrsaayg ppvsayvtph
     2101 gpnltvdpta yqqyihslya lqppphhhrp swl
//
LOCUS       XP_034183865            1323 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 47 [Osmia lignaria lignaria].
ACCESSION   XP_034183865
VERSION     XP_034183865.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327974.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183865.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="ubiquitin specific protease 47"
                     /calculated_mol_wt=152027
     Region          153..557
                     /region_name="peptidase_C19C"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02659"
                     /db_xref="CDD:239124"
     Site            order(158,163,497,515)
                     /site_type="active"
                     /db_xref="CDD:239124"
     Region          1088..1318
                     /region_name="USP47_C"
                     /note="Ubiquitin carboxyl-terminal hydrolase 47
                     C-terminal; pfam19718"
                     /db_xref="CDD:466158"
     CDS             1..1323
                     /gene="Usp47"
                     /coded_by="XM_034327974.2:229..4200"
                     /db_xref="GeneID:117605996"
ORIGIN      
        1 mvciaglpcv ikfnlyngak dknlsniefv lnestrgrdl ydyiqecgqi qpdsfklyll
       61 ssevmidlse mkdktmseig vdfsyardst kskytlvvld ststsfctns lvqkqvecnl
      121 ptynppppsp nhssvwsegn vdfkslnkpe icyvglvnqa mtcylnsllq alymtpefrn
      181 alynweyveg sekdeansip yqlqklflnl qtsaksavet taltksfgwd steawqqhdi
      241 qelcrvmfda leqkfknteq adlinrlyeg kmidyvkcle csteksredt fldiplpvrp
      301 fgsnvayssv eealrafvqy etlegnnqyf cekcnkkcda hkglkftkfp ylltlhlkrf
      361 dfdfktfhri klndkvtfpd ilnlnpfiss ipnqespssd eniglvkcdd ssttdsgtld
      421 ddyvlcennl snsnhstnhd qdddegidms ngpstsnctv hnheneknrn sssaakgpyn
      481 yelfsimihs gsasgghyya yikdfrtqew lcfndqsvtq ithddiqkty gggpsrpyrv
      541 ssscanayml myrqidpvrn alpmqvqdfp khiqellkkm kenedndrkt risqheshtl
      601 riphdenrms qheshtlrmc vplyetlasn skshmsqlds rigyftrkvk vhcirpneni
      661 llshrlplpp datwaettkq vykdfrleki vdldqcrlva ydeynheiva syegrenetf
      721 esisnnrins llleirdkdk kfesyprgiv tkvfivdvpt reliegpiiv ralltqtvke
      781 ykqmvgkkin idlsemevvl rkhgtdttlv snsdvdlqaa gfcnlvnvfm ssmldteykp
      841 ffntaicgiv eeleniitlr iklpdtsket ledlnipple eepkdkekpv vstspskvsv
      901 ndsnhdagpk snevsktgkt tedtpvrnps pqlgegeewn tpeqsnseds slsdsdktlv
      961 gdapeddvfq lpylpsknra ikmlnhigle nwdnviddse yyvkvtpyms syrkclkvlv
     1021 dkrmtfstlk khlepyvgvr sdyfkifryf nesgesqcsl fnrlvsyede eelciklgra
     1081 lrngeftvkl yqffidspkp ykflcewils egmqvgqakk eflaeikrky didipyercr
     1141 lrkkcfskat kvflddqkfe lpfypqvemi iqelpdkepi tngnqvvlfv rrwsvetmql
     1201 aplqeivldr ksaeelykkl ssisdipekh ievakakasf sytsivriqn elewnetmid
     1261 iepsiqdgae yiyrdnretv knltreefse iavqevsrll ppssaprysa rrekalrinl
     1321 dte
//
LOCUS       XP_034183817            1323 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 47 [Osmia lignaria lignaria].
ACCESSION   XP_034183817
VERSION     XP_034183817.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327926.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183817.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="ubiquitin specific protease 47"
                     /calculated_mol_wt=152027
     Region          153..557
                     /region_name="peptidase_C19C"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02659"
                     /db_xref="CDD:239124"
     Site            order(158,163,497,515)
                     /site_type="active"
                     /db_xref="CDD:239124"
     Region          1088..1318
                     /region_name="USP47_C"
                     /note="Ubiquitin carboxyl-terminal hydrolase 47
                     C-terminal; pfam19718"
                     /db_xref="CDD:466158"
     CDS             1..1323
                     /gene="Usp47"
                     /coded_by="XM_034327926.2:885..4856"
                     /db_xref="GeneID:117605996"
ORIGIN      
        1 mvciaglpcv ikfnlyngak dknlsniefv lnestrgrdl ydyiqecgqi qpdsfklyll
       61 ssevmidlse mkdktmseig vdfsyardst kskytlvvld ststsfctns lvqkqvecnl
      121 ptynppppsp nhssvwsegn vdfkslnkpe icyvglvnqa mtcylnsllq alymtpefrn
      181 alynweyveg sekdeansip yqlqklflnl qtsaksavet taltksfgwd steawqqhdi
      241 qelcrvmfda leqkfknteq adlinrlyeg kmidyvkcle csteksredt fldiplpvrp
      301 fgsnvayssv eealrafvqy etlegnnqyf cekcnkkcda hkglkftkfp ylltlhlkrf
      361 dfdfktfhri klndkvtfpd ilnlnpfiss ipnqespssd eniglvkcdd ssttdsgtld
      421 ddyvlcennl snsnhstnhd qdddegidms ngpstsnctv hnheneknrn sssaakgpyn
      481 yelfsimihs gsasgghyya yikdfrtqew lcfndqsvtq ithddiqkty gggpsrpyrv
      541 ssscanayml myrqidpvrn alpmqvqdfp khiqellkkm kenedndrkt risqheshtl
      601 riphdenrms qheshtlrmc vplyetlasn skshmsqlds rigyftrkvk vhcirpneni
      661 llshrlplpp datwaettkq vykdfrleki vdldqcrlva ydeynheiva syegrenetf
      721 esisnnrins llleirdkdk kfesyprgiv tkvfivdvpt reliegpiiv ralltqtvke
      781 ykqmvgkkin idlsemevvl rkhgtdttlv snsdvdlqaa gfcnlvnvfm ssmldteykp
      841 ffntaicgiv eeleniitlr iklpdtsket ledlnipple eepkdkekpv vstspskvsv
      901 ndsnhdagpk snevsktgkt tedtpvrnps pqlgegeewn tpeqsnseds slsdsdktlv
      961 gdapeddvfq lpylpsknra ikmlnhigle nwdnviddse yyvkvtpyms syrkclkvlv
     1021 dkrmtfstlk khlepyvgvr sdyfkifryf nesgesqcsl fnrlvsyede eelciklgra
     1081 lrngeftvkl yqffidspkp ykflcewils egmqvgqakk eflaeikrky didipyercr
     1141 lrkkcfskat kvflddqkfe lpfypqvemi iqelpdkepi tngnqvvlfv rrwsvetmql
     1201 aplqeivldr ksaeelykkl ssisdipekh ievakakasf sytsivriqn elewnetmid
     1261 iepsiqdgae yiyrdnretv knltreefse iavqevsrll ppssaprysa rrekalrinl
     1321 dte
//
LOCUS       XP_034183833            1323 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 47 [Osmia lignaria lignaria].
ACCESSION   XP_034183833
VERSION     XP_034183833.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327942.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183833.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="ubiquitin specific protease 47"
                     /calculated_mol_wt=152027
     Region          153..557
                     /region_name="peptidase_C19C"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02659"
                     /db_xref="CDD:239124"
     Site            order(158,163,497,515)
                     /site_type="active"
                     /db_xref="CDD:239124"
     Region          1088..1318
                     /region_name="USP47_C"
                     /note="Ubiquitin carboxyl-terminal hydrolase 47
                     C-terminal; pfam19718"
                     /db_xref="CDD:466158"
     CDS             1..1323
                     /gene="Usp47"
                     /coded_by="XM_034327942.2:233..4204"
                     /db_xref="GeneID:117605996"
ORIGIN      
        1 mvciaglpcv ikfnlyngak dknlsniefv lnestrgrdl ydyiqecgqi qpdsfklyll
       61 ssevmidlse mkdktmseig vdfsyardst kskytlvvld ststsfctns lvqkqvecnl
      121 ptynppppsp nhssvwsegn vdfkslnkpe icyvglvnqa mtcylnsllq alymtpefrn
      181 alynweyveg sekdeansip yqlqklflnl qtsaksavet taltksfgwd steawqqhdi
      241 qelcrvmfda leqkfknteq adlinrlyeg kmidyvkcle csteksredt fldiplpvrp
      301 fgsnvayssv eealrafvqy etlegnnqyf cekcnkkcda hkglkftkfp ylltlhlkrf
      361 dfdfktfhri klndkvtfpd ilnlnpfiss ipnqespssd eniglvkcdd ssttdsgtld
      421 ddyvlcennl snsnhstnhd qdddegidms ngpstsnctv hnheneknrn sssaakgpyn
      481 yelfsimihs gsasgghyya yikdfrtqew lcfndqsvtq ithddiqkty gggpsrpyrv
      541 ssscanayml myrqidpvrn alpmqvqdfp khiqellkkm kenedndrkt risqheshtl
      601 riphdenrms qheshtlrmc vplyetlasn skshmsqlds rigyftrkvk vhcirpneni
      661 llshrlplpp datwaettkq vykdfrleki vdldqcrlva ydeynheiva syegrenetf
      721 esisnnrins llleirdkdk kfesyprgiv tkvfivdvpt reliegpiiv ralltqtvke
      781 ykqmvgkkin idlsemevvl rkhgtdttlv snsdvdlqaa gfcnlvnvfm ssmldteykp
      841 ffntaicgiv eeleniitlr iklpdtsket ledlnipple eepkdkekpv vstspskvsv
      901 ndsnhdagpk snevsktgkt tedtpvrnps pqlgegeewn tpeqsnseds slsdsdktlv
      961 gdapeddvfq lpylpsknra ikmlnhigle nwdnviddse yyvkvtpyms syrkclkvlv
     1021 dkrmtfstlk khlepyvgvr sdyfkifryf nesgesqcsl fnrlvsyede eelciklgra
     1081 lrngeftvkl yqffidspkp ykflcewils egmqvgqakk eflaeikrky didipyercr
     1141 lrkkcfskat kvflddqkfe lpfypqvemi iqelpdkepi tngnqvvlfv rrwsvetmql
     1201 aplqeivldr ksaeelykkl ssisdipekh ievakakasf sytsivriqn elewnetmid
     1261 iepsiqdgae yiyrdnretv knltreefse iavqevsrll ppssaprysa rrekalrinl
     1321 dte
//
LOCUS       XP_034183826            1323 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 47 [Osmia lignaria lignaria].
ACCESSION   XP_034183826
VERSION     XP_034183826.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327935.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183826.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="ubiquitin specific protease 47"
                     /calculated_mol_wt=152027
     Region          153..557
                     /region_name="peptidase_C19C"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02659"
                     /db_xref="CDD:239124"
     Site            order(158,163,497,515)
                     /site_type="active"
                     /db_xref="CDD:239124"
     Region          1088..1318
                     /region_name="USP47_C"
                     /note="Ubiquitin carboxyl-terminal hydrolase 47
                     C-terminal; pfam19718"
                     /db_xref="CDD:466158"
     CDS             1..1323
                     /gene="Usp47"
                     /coded_by="XM_034327935.2:241..4212"
                     /db_xref="GeneID:117605996"
ORIGIN      
        1 mvciaglpcv ikfnlyngak dknlsniefv lnestrgrdl ydyiqecgqi qpdsfklyll
       61 ssevmidlse mkdktmseig vdfsyardst kskytlvvld ststsfctns lvqkqvecnl
      121 ptynppppsp nhssvwsegn vdfkslnkpe icyvglvnqa mtcylnsllq alymtpefrn
      181 alynweyveg sekdeansip yqlqklflnl qtsaksavet taltksfgwd steawqqhdi
      241 qelcrvmfda leqkfknteq adlinrlyeg kmidyvkcle csteksredt fldiplpvrp
      301 fgsnvayssv eealrafvqy etlegnnqyf cekcnkkcda hkglkftkfp ylltlhlkrf
      361 dfdfktfhri klndkvtfpd ilnlnpfiss ipnqespssd eniglvkcdd ssttdsgtld
      421 ddyvlcennl snsnhstnhd qdddegidms ngpstsnctv hnheneknrn sssaakgpyn
      481 yelfsimihs gsasgghyya yikdfrtqew lcfndqsvtq ithddiqkty gggpsrpyrv
      541 ssscanayml myrqidpvrn alpmqvqdfp khiqellkkm kenedndrkt risqheshtl
      601 riphdenrms qheshtlrmc vplyetlasn skshmsqlds rigyftrkvk vhcirpneni
      661 llshrlplpp datwaettkq vykdfrleki vdldqcrlva ydeynheiva syegrenetf
      721 esisnnrins llleirdkdk kfesyprgiv tkvfivdvpt reliegpiiv ralltqtvke
      781 ykqmvgkkin idlsemevvl rkhgtdttlv snsdvdlqaa gfcnlvnvfm ssmldteykp
      841 ffntaicgiv eeleniitlr iklpdtsket ledlnipple eepkdkekpv vstspskvsv
      901 ndsnhdagpk snevsktgkt tedtpvrnps pqlgegeewn tpeqsnseds slsdsdktlv
      961 gdapeddvfq lpylpsknra ikmlnhigle nwdnviddse yyvkvtpyms syrkclkvlv
     1021 dkrmtfstlk khlepyvgvr sdyfkifryf nesgesqcsl fnrlvsyede eelciklgra
     1081 lrngeftvkl yqffidspkp ykflcewils egmqvgqakk eflaeikrky didipyercr
     1141 lrkkcfskat kvflddqkfe lpfypqvemi iqelpdkepi tngnqvvlfv rrwsvetmql
     1201 aplqeivldr ksaeelykkl ssisdipekh ievakakasf sytsivriqn elewnetmid
     1261 iepsiqdgae yiyrdnretv knltreefse iavqevsrll ppssaprysa rrekalrinl
     1321 dte
//
LOCUS       XP_034183840            1323 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 47 [Osmia lignaria lignaria].
ACCESSION   XP_034183840
VERSION     XP_034183840.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327949.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183840.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="ubiquitin specific protease 47"
                     /calculated_mol_wt=152027
     Region          153..557
                     /region_name="peptidase_C19C"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02659"
                     /db_xref="CDD:239124"
     Site            order(158,163,497,515)
                     /site_type="active"
                     /db_xref="CDD:239124"
     Region          1088..1318
                     /region_name="USP47_C"
                     /note="Ubiquitin carboxyl-terminal hydrolase 47
                     C-terminal; pfam19718"
                     /db_xref="CDD:466158"
     CDS             1..1323
                     /gene="Usp47"
                     /coded_by="XM_034327949.2:196..4167"
                     /db_xref="GeneID:117605996"
ORIGIN      
        1 mvciaglpcv ikfnlyngak dknlsniefv lnestrgrdl ydyiqecgqi qpdsfklyll
       61 ssevmidlse mkdktmseig vdfsyardst kskytlvvld ststsfctns lvqkqvecnl
      121 ptynppppsp nhssvwsegn vdfkslnkpe icyvglvnqa mtcylnsllq alymtpefrn
      181 alynweyveg sekdeansip yqlqklflnl qtsaksavet taltksfgwd steawqqhdi
      241 qelcrvmfda leqkfknteq adlinrlyeg kmidyvkcle csteksredt fldiplpvrp
      301 fgsnvayssv eealrafvqy etlegnnqyf cekcnkkcda hkglkftkfp ylltlhlkrf
      361 dfdfktfhri klndkvtfpd ilnlnpfiss ipnqespssd eniglvkcdd ssttdsgtld
      421 ddyvlcennl snsnhstnhd qdddegidms ngpstsnctv hnheneknrn sssaakgpyn
      481 yelfsimihs gsasgghyya yikdfrtqew lcfndqsvtq ithddiqkty gggpsrpyrv
      541 ssscanayml myrqidpvrn alpmqvqdfp khiqellkkm kenedndrkt risqheshtl
      601 riphdenrms qheshtlrmc vplyetlasn skshmsqlds rigyftrkvk vhcirpneni
      661 llshrlplpp datwaettkq vykdfrleki vdldqcrlva ydeynheiva syegrenetf
      721 esisnnrins llleirdkdk kfesyprgiv tkvfivdvpt reliegpiiv ralltqtvke
      781 ykqmvgkkin idlsemevvl rkhgtdttlv snsdvdlqaa gfcnlvnvfm ssmldteykp
      841 ffntaicgiv eeleniitlr iklpdtsket ledlnipple eepkdkekpv vstspskvsv
      901 ndsnhdagpk snevsktgkt tedtpvrnps pqlgegeewn tpeqsnseds slsdsdktlv
      961 gdapeddvfq lpylpsknra ikmlnhigle nwdnviddse yyvkvtpyms syrkclkvlv
     1021 dkrmtfstlk khlepyvgvr sdyfkifryf nesgesqcsl fnrlvsyede eelciklgra
     1081 lrngeftvkl yqffidspkp ykflcewils egmqvgqakk eflaeikrky didipyercr
     1141 lrkkcfskat kvflddqkfe lpfypqvemi iqelpdkepi tngnqvvlfv rrwsvetmql
     1201 aplqeivldr ksaeelykkl ssisdipekh ievakakasf sytsivriqn elewnetmid
     1261 iepsiqdgae yiyrdnretv knltreefse iavqevsrll ppssaprysa rrekalrinl
     1321 dte
//
LOCUS       XP_034183850            1323 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 47 [Osmia lignaria lignaria].
ACCESSION   XP_034183850
VERSION     XP_034183850.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327959.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183850.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="ubiquitin specific protease 47"
                     /calculated_mol_wt=152027
     Region          153..557
                     /region_name="peptidase_C19C"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02659"
                     /db_xref="CDD:239124"
     Site            order(158,163,497,515)
                     /site_type="active"
                     /db_xref="CDD:239124"
     Region          1088..1318
                     /region_name="USP47_C"
                     /note="Ubiquitin carboxyl-terminal hydrolase 47
                     C-terminal; pfam19718"
                     /db_xref="CDD:466158"
     CDS             1..1323
                     /gene="Usp47"
                     /coded_by="XM_034327959.2:194..4165"
                     /db_xref="GeneID:117605996"
ORIGIN      
        1 mvciaglpcv ikfnlyngak dknlsniefv lnestrgrdl ydyiqecgqi qpdsfklyll
       61 ssevmidlse mkdktmseig vdfsyardst kskytlvvld ststsfctns lvqkqvecnl
      121 ptynppppsp nhssvwsegn vdfkslnkpe icyvglvnqa mtcylnsllq alymtpefrn
      181 alynweyveg sekdeansip yqlqklflnl qtsaksavet taltksfgwd steawqqhdi
      241 qelcrvmfda leqkfknteq adlinrlyeg kmidyvkcle csteksredt fldiplpvrp
      301 fgsnvayssv eealrafvqy etlegnnqyf cekcnkkcda hkglkftkfp ylltlhlkrf
      361 dfdfktfhri klndkvtfpd ilnlnpfiss ipnqespssd eniglvkcdd ssttdsgtld
      421 ddyvlcennl snsnhstnhd qdddegidms ngpstsnctv hnheneknrn sssaakgpyn
      481 yelfsimihs gsasgghyya yikdfrtqew lcfndqsvtq ithddiqkty gggpsrpyrv
      541 ssscanayml myrqidpvrn alpmqvqdfp khiqellkkm kenedndrkt risqheshtl
      601 riphdenrms qheshtlrmc vplyetlasn skshmsqlds rigyftrkvk vhcirpneni
      661 llshrlplpp datwaettkq vykdfrleki vdldqcrlva ydeynheiva syegrenetf
      721 esisnnrins llleirdkdk kfesyprgiv tkvfivdvpt reliegpiiv ralltqtvke
      781 ykqmvgkkin idlsemevvl rkhgtdttlv snsdvdlqaa gfcnlvnvfm ssmldteykp
      841 ffntaicgiv eeleniitlr iklpdtsket ledlnipple eepkdkekpv vstspskvsv
      901 ndsnhdagpk snevsktgkt tedtpvrnps pqlgegeewn tpeqsnseds slsdsdktlv
      961 gdapeddvfq lpylpsknra ikmlnhigle nwdnviddse yyvkvtpyms syrkclkvlv
     1021 dkrmtfstlk khlepyvgvr sdyfkifryf nesgesqcsl fnrlvsyede eelciklgra
     1081 lrngeftvkl yqffidspkp ykflcewils egmqvgqakk eflaeikrky didipyercr
     1141 lrkkcfskat kvflddqkfe lpfypqvemi iqelpdkepi tngnqvvlfv rrwsvetmql
     1201 aplqeivldr ksaeelykkl ssisdipekh ievakakasf sytsivriqn elewnetmid
     1261 iepsiqdgae yiyrdnretv knltreefse iavqevsrll ppssaprysa rrekalrinl
     1321 dte
//
LOCUS       XP_034183856            1323 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 47 [Osmia lignaria lignaria].
ACCESSION   XP_034183856
VERSION     XP_034183856.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327965.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183856.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1323
                     /product="ubiquitin specific protease 47"
                     /calculated_mol_wt=152027
     Region          153..557
                     /region_name="peptidase_C19C"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02659"
                     /db_xref="CDD:239124"
     Site            order(158,163,497,515)
                     /site_type="active"
                     /db_xref="CDD:239124"
     Region          1088..1318
                     /region_name="USP47_C"
                     /note="Ubiquitin carboxyl-terminal hydrolase 47
                     C-terminal; pfam19718"
                     /db_xref="CDD:466158"
     CDS             1..1323
                     /gene="Usp47"
                     /coded_by="XM_034327965.2:458..4429"
                     /db_xref="GeneID:117605996"
ORIGIN      
        1 mvciaglpcv ikfnlyngak dknlsniefv lnestrgrdl ydyiqecgqi qpdsfklyll
       61 ssevmidlse mkdktmseig vdfsyardst kskytlvvld ststsfctns lvqkqvecnl
      121 ptynppppsp nhssvwsegn vdfkslnkpe icyvglvnqa mtcylnsllq alymtpefrn
      181 alynweyveg sekdeansip yqlqklflnl qtsaksavet taltksfgwd steawqqhdi
      241 qelcrvmfda leqkfknteq adlinrlyeg kmidyvkcle csteksredt fldiplpvrp
      301 fgsnvayssv eealrafvqy etlegnnqyf cekcnkkcda hkglkftkfp ylltlhlkrf
      361 dfdfktfhri klndkvtfpd ilnlnpfiss ipnqespssd eniglvkcdd ssttdsgtld
      421 ddyvlcennl snsnhstnhd qdddegidms ngpstsnctv hnheneknrn sssaakgpyn
      481 yelfsimihs gsasgghyya yikdfrtqew lcfndqsvtq ithddiqkty gggpsrpyrv
      541 ssscanayml myrqidpvrn alpmqvqdfp khiqellkkm kenedndrkt risqheshtl
      601 riphdenrms qheshtlrmc vplyetlasn skshmsqlds rigyftrkvk vhcirpneni
      661 llshrlplpp datwaettkq vykdfrleki vdldqcrlva ydeynheiva syegrenetf
      721 esisnnrins llleirdkdk kfesyprgiv tkvfivdvpt reliegpiiv ralltqtvke
      781 ykqmvgkkin idlsemevvl rkhgtdttlv snsdvdlqaa gfcnlvnvfm ssmldteykp
      841 ffntaicgiv eeleniitlr iklpdtsket ledlnipple eepkdkekpv vstspskvsv
      901 ndsnhdagpk snevsktgkt tedtpvrnps pqlgegeewn tpeqsnseds slsdsdktlv
      961 gdapeddvfq lpylpsknra ikmlnhigle nwdnviddse yyvkvtpyms syrkclkvlv
     1021 dkrmtfstlk khlepyvgvr sdyfkifryf nesgesqcsl fnrlvsyede eelciklgra
     1081 lrngeftvkl yqffidspkp ykflcewils egmqvgqakk eflaeikrky didipyercr
     1141 lrkkcfskat kvflddqkfe lpfypqvemi iqelpdkepi tngnqvvlfv rrwsvetmql
     1201 aplqeivldr ksaeelykkl ssisdipekh ievakakasf sytsivriqn elewnetmid
     1261 iepsiqdgae yiyrdnretv knltreefse iavqevsrll ppssaprysa rrekalrinl
     1321 dte
//
LOCUS       XP_034183877            1051 aa            linear   INV 03-SEP-2025
DEFINITION  tuberous sclerosis 1 protein hamartin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183877
VERSION     XP_034183877.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183877.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1051
                     /product="tuberous sclerosis 1 protein hamartin isoform
                     X1"
                     /calculated_mol_wt=118298
     Region          8..712
                     /region_name="Hamartin"
                     /note="Hamartin protein; pfam04388"
                     /db_xref="CDD:461287"
     Region          <646..958
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1051
                     /gene="Tsc1"
                     /coded_by="XM_034327986.2:292..3447"
                     /db_xref="GeneID:117606024"
ORIGIN      
        1 mninsigiad ifnllesnkl aeveeikkvf hefflctkdn wlvnglfdyy lstnslrave
       61 vlagvrdphd khlldrlsea lsksgsnsqr iqtltllghi arrqptwlyk ltshtlfrdl
      121 lkllkmeadt lslmsallll vmlvpmlpaa lgpylseife vfgrlasyyh yqsssvsssp
      181 mhftsttvtg tidknhlyll hlqvglyslf hrlyalypcn fisylkqqym qreqlatfth
      241 tvrpmldsvr mhpllvtask dteisatrwk kmehhdvmae cgrftldkcr eevfsttnsr
      301 stppldyssv cipistgggi asseiinged dtfwspsmtv ppqspqfpat sheqrsapst
      361 pnnrsgtspp eaaveatpet tpvkdlrkls trqiptgsaa vraltafgng tvgpssrpst
      421 pvpvnppvsg sligggdgnn ahgllshkis kimadrqtit qqksfvnvde dgrisetdtn
      481 qngkndswqe dqevleivss qaagkfessk yierqeqhne kfqnaftdls qrvyqlrlys
      541 qsqasehftn snslykirks kscpdieiqk qighnktnqe tiimgakklq etgtqtfell
      601 pyeylllgil dqktrmnvqn hiqkdtksrl spttmldryv eactrisnfs gdktktntmk
      661 qkqkkendge sevaeedald iecatqllql mqmqllferq rrevhaernr rllgklrdsr
      721 aleehnsalt nrlkimeeev eglkaelern kkeackieni yketvhhwqi kcveeqqqnl
      781 tlknrlesve leltneqkkv sdceqrlrst easlfdaghq lrvalkaadr skelertldt
      841 vqkkflllge aqlklkestd glspmtkqea tqiqksyaee ltnlrrqves rtsliealkv
      901 rlselenkda rkeaqlvelq rllqetkdqh eaeleavesk ykaqveinll legrilelhg
      961 tletatcgtt tlnnlassas pkerspplsa slasssegsl afihsstgii mndccdtage
     1021 ipnlqaivep apsqatspsr qnqqrrnpkq e
//
LOCUS       XP_034183886            1051 aa            linear   INV 03-SEP-2025
DEFINITION  tuberous sclerosis 1 protein hamartin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183886
VERSION     XP_034183886.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327995.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183886.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1051
                     /product="tuberous sclerosis 1 protein hamartin isoform
                     X1"
                     /calculated_mol_wt=118298
     Region          8..712
                     /region_name="Hamartin"
                     /note="Hamartin protein; pfam04388"
                     /db_xref="CDD:461287"
     Region          <646..958
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1051
                     /gene="Tsc1"
                     /coded_by="XM_034327995.2:433..3588"
                     /db_xref="GeneID:117606024"
ORIGIN      
        1 mninsigiad ifnllesnkl aeveeikkvf hefflctkdn wlvnglfdyy lstnslrave
       61 vlagvrdphd khlldrlsea lsksgsnsqr iqtltllghi arrqptwlyk ltshtlfrdl
      121 lkllkmeadt lslmsallll vmlvpmlpaa lgpylseife vfgrlasyyh yqsssvsssp
      181 mhftsttvtg tidknhlyll hlqvglyslf hrlyalypcn fisylkqqym qreqlatfth
      241 tvrpmldsvr mhpllvtask dteisatrwk kmehhdvmae cgrftldkcr eevfsttnsr
      301 stppldyssv cipistgggi asseiinged dtfwspsmtv ppqspqfpat sheqrsapst
      361 pnnrsgtspp eaaveatpet tpvkdlrkls trqiptgsaa vraltafgng tvgpssrpst
      421 pvpvnppvsg sligggdgnn ahgllshkis kimadrqtit qqksfvnvde dgrisetdtn
      481 qngkndswqe dqevleivss qaagkfessk yierqeqhne kfqnaftdls qrvyqlrlys
      541 qsqasehftn snslykirks kscpdieiqk qighnktnqe tiimgakklq etgtqtfell
      601 pyeylllgil dqktrmnvqn hiqkdtksrl spttmldryv eactrisnfs gdktktntmk
      661 qkqkkendge sevaeedald iecatqllql mqmqllferq rrevhaernr rllgklrdsr
      721 aleehnsalt nrlkimeeev eglkaelern kkeackieni yketvhhwqi kcveeqqqnl
      781 tlknrlesve leltneqkkv sdceqrlrst easlfdaghq lrvalkaadr skelertldt
      841 vqkkflllge aqlklkestd glspmtkqea tqiqksyaee ltnlrrqves rtsliealkv
      901 rlselenkda rkeaqlvelq rllqetkdqh eaeleavesk ykaqveinll legrilelhg
      961 tletatcgtt tlnnlassas pkerspplsa slasssegsl afihsstgii mndccdtage
     1021 ipnlqaivep apsqatspsr qnqqrrnpkq e
//
LOCUS       XP_076546463            1051 aa            linear   INV 03-SEP-2025
DEFINITION  tuberous sclerosis 1 protein hamartin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546463
VERSION     XP_076546463.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690348.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1051
                     /product="tuberous sclerosis 1 protein hamartin isoform
                     X1"
                     /calculated_mol_wt=118298
     Region          8..712
                     /region_name="Hamartin"
                     /note="Hamartin protein; pfam04388"
                     /db_xref="CDD:461287"
     Region          <646..958
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1051
                     /gene="Tsc1"
                     /coded_by="XM_076690348.1:303..3458"
                     /db_xref="GeneID:117606024"
ORIGIN      
        1 mninsigiad ifnllesnkl aeveeikkvf hefflctkdn wlvnglfdyy lstnslrave
       61 vlagvrdphd khlldrlsea lsksgsnsqr iqtltllghi arrqptwlyk ltshtlfrdl
      121 lkllkmeadt lslmsallll vmlvpmlpaa lgpylseife vfgrlasyyh yqsssvsssp
      181 mhftsttvtg tidknhlyll hlqvglyslf hrlyalypcn fisylkqqym qreqlatfth
      241 tvrpmldsvr mhpllvtask dteisatrwk kmehhdvmae cgrftldkcr eevfsttnsr
      301 stppldyssv cipistgggi asseiinged dtfwspsmtv ppqspqfpat sheqrsapst
      361 pnnrsgtspp eaaveatpet tpvkdlrkls trqiptgsaa vraltafgng tvgpssrpst
      421 pvpvnppvsg sligggdgnn ahgllshkis kimadrqtit qqksfvnvde dgrisetdtn
      481 qngkndswqe dqevleivss qaagkfessk yierqeqhne kfqnaftdls qrvyqlrlys
      541 qsqasehftn snslykirks kscpdieiqk qighnktnqe tiimgakklq etgtqtfell
      601 pyeylllgil dqktrmnvqn hiqkdtksrl spttmldryv eactrisnfs gdktktntmk
      661 qkqkkendge sevaeedald iecatqllql mqmqllferq rrevhaernr rllgklrdsr
      721 aleehnsalt nrlkimeeev eglkaelern kkeackieni yketvhhwqi kcveeqqqnl
      781 tlknrlesve leltneqkkv sdceqrlrst easlfdaghq lrvalkaadr skelertldt
      841 vqkkflllge aqlklkestd glspmtkqea tqiqksyaee ltnlrrqves rtsliealkv
      901 rlselenkda rkeaqlvelq rllqetkdqh eaeleavesk ykaqveinll legrilelhg
      961 tletatcgtt tlnnlassas pkerspplsa slasssegsl afihsstgii mndccdtage
     1021 ipnlqaivep apsqatspsr qnqqrrnpkq e
//
LOCUS       XP_034183894            1051 aa            linear   INV 03-SEP-2025
DEFINITION  tuberous sclerosis 1 protein hamartin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183894
VERSION     XP_034183894.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183894.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1051
                     /product="tuberous sclerosis 1 protein hamartin isoform
                     X1"
                     /calculated_mol_wt=118298
     Region          8..712
                     /region_name="Hamartin"
                     /note="Hamartin protein; pfam04388"
                     /db_xref="CDD:461287"
     Region          <646..958
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1051
                     /gene="Tsc1"
                     /coded_by="XM_034328003.2:296..3451"
                     /db_xref="GeneID:117606024"
ORIGIN      
        1 mninsigiad ifnllesnkl aeveeikkvf hefflctkdn wlvnglfdyy lstnslrave
       61 vlagvrdphd khlldrlsea lsksgsnsqr iqtltllghi arrqptwlyk ltshtlfrdl
      121 lkllkmeadt lslmsallll vmlvpmlpaa lgpylseife vfgrlasyyh yqsssvsssp
      181 mhftsttvtg tidknhlyll hlqvglyslf hrlyalypcn fisylkqqym qreqlatfth
      241 tvrpmldsvr mhpllvtask dteisatrwk kmehhdvmae cgrftldkcr eevfsttnsr
      301 stppldyssv cipistgggi asseiinged dtfwspsmtv ppqspqfpat sheqrsapst
      361 pnnrsgtspp eaaveatpet tpvkdlrkls trqiptgsaa vraltafgng tvgpssrpst
      421 pvpvnppvsg sligggdgnn ahgllshkis kimadrqtit qqksfvnvde dgrisetdtn
      481 qngkndswqe dqevleivss qaagkfessk yierqeqhne kfqnaftdls qrvyqlrlys
      541 qsqasehftn snslykirks kscpdieiqk qighnktnqe tiimgakklq etgtqtfell
      601 pyeylllgil dqktrmnvqn hiqkdtksrl spttmldryv eactrisnfs gdktktntmk
      661 qkqkkendge sevaeedald iecatqllql mqmqllferq rrevhaernr rllgklrdsr
      721 aleehnsalt nrlkimeeev eglkaelern kkeackieni yketvhhwqi kcveeqqqnl
      781 tlknrlesve leltneqkkv sdceqrlrst easlfdaghq lrvalkaadr skelertldt
      841 vqkkflllge aqlklkestd glspmtkqea tqiqksyaee ltnlrrqves rtsliealkv
      901 rlselenkda rkeaqlvelq rllqetkdqh eaeleavesk ykaqveinll legrilelhg
      961 tletatcgtt tlnnlassas pkerspplsa slasssegsl afihsstgii mndccdtage
     1021 ipnlqaivep apsqatspsr qnqqrrnpkq e
//
LOCUS       XP_034183912            1017 aa            linear   INV 03-SEP-2025
DEFINITION  tuberous sclerosis 1 protein hamartin isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183912
VERSION     XP_034183912.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183912.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1017
                     /product="tuberous sclerosis 1 protein hamartin isoform
                     X2"
                     /calculated_mol_wt=114437
     Region          8..678
                     /region_name="Hamartin"
                     /note="Hamartin protein; pfam04388"
                     /db_xref="CDD:461287"
     Region          <612..924
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1017
                     /gene="Tsc1"
                     /coded_by="XM_034328021.2:292..3345"
                     /db_xref="GeneID:117606024"
ORIGIN      
        1 mninsigiad ifnllesnkl aeveeikkvf hefflctkdn wlvnglfdyy lstnslrave
       61 vlagvrdphd khlldrlsea lsksgsnsqr iqtltllghi arrqptwlyk ltshtlfrdl
      121 lkllkmeadt lslmsallll vmlvpmlpaa lgpylseife vfgrlasyyh yqsssvsssp
      181 mhftsttvtg tidknhlyll hlqvglyslf hrlyalypcn fisylkqqym qreqlatfth
      241 tvrpmldsvr mhpllvtask dteisatrwk kmehhdvmae cgrftldkcr eevfsttnsr
      301 stppldyssv cipistgggi asseiinged dtfwspsmtv ppqspqfpat sheqrsapst
      361 pnnrsgtspp eaaveatpet tpvkdlrkls trqiptgsaa vraltafgng tvgpssrpst
      421 pvpvnppvsg sligggdgnn ahgllshkis kimadrqtit qqksfvnvde dgrisetdtn
      481 qngkndswqe dqevleivss qaagkfessk yierqeqhne kfqnaftdls qrvyqlrlys
      541 qsqasehftn snslyketgt qtfellpyey lllgildqkt rmnvqnhiqk dtksrlsptt
      601 mldryveact risnfsgdkt ktntmkqkqk kendgeseva eedaldieca tqllqlmqmq
      661 llferqrrev haernrrllg klrdsralee hnsaltnrlk imeeeveglk aelernkkea
      721 ckieniyket vhhwqikcve eqqqnltlkn rlesvelelt neqkkvsdce qrlrsteasl
      781 fdaghqlrva lkaadrskel ertldtvqkk flllgeaqlk lkestdglsp mtkqeatqiq
      841 ksyaeeltnl rrqvesrtsl iealkvrlse lenkdarkea qlvelqrllq etkdqheael
      901 eaveskykaq veinlllegr ilelhgtlet atcgtttlnn lassaspker spplsaslas
      961 ssegslafih sstgiimndc cdtageipnl qaivepapsq atspsrqnqq rrnpkqe
//
LOCUS       XP_034172784             625 aa            linear   INV 03-SEP-2025
DEFINITION  ran GTPase activating protein [Osmia lignaria lignaria].
ACCESSION   XP_034172784
VERSION     XP_034172784.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172784.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..625
                     /product="ran GTPase activating protein"
                     /calculated_mol_wt=68114
     Region          33..351
                     /region_name="LRR_RI"
                     /note="Leucine-rich repeats (LRRs), ribonuclease inhibitor
                     (RI)-like subfamily. LRRs are 20-29 residue sequence
                     motifs present in many proteins that participate in
                     protein-protein interactions and have different functions
                     and cellular locations. LRRs correspond...; cd00116"
                     /db_xref="CDD:238064"
     Site            order(33,35,53,56,58,61,63,82,85,87,90,92,117,120,122,125,
                     127,147,150,152,155,157,186,189,191,194,196,214,217,219,
                     222,224,242,245,247,250,252,270,273,275,278,280,301,304,
                     306,309,311,329,332,334,337,339)
                     /site_type="active"
                     /note="Leucine-rich repeats [active]"
                     /db_xref="CDD:238064"
     Region          53..84
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Site            order(59..60,124,193,221,277,305,307,333)
                     /site_type="other"
                     /note="Substrate binding site [chemical binding]"
                     /db_xref="CDD:238064"
     Region          85..116
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          117..146
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          147..185
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          186..213
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          214..241
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          242..269
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          270..300
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          301..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          421..614
                     /region_name="RanGAP1_C"
                     /note="RanGAP1 C-terminal domain; pfam07834"
                     /db_xref="CDD:462282"
     CDS             1..625
                     /gene="RanGAP"
                     /coded_by="XM_034316893.2:163..2040"
                     /db_xref="GeneID:117600884"
ORIGIN      
        1 mssfnlndlg aqlkdvtqns tgigvsfakk slkldskeda mevieaiqgc snleyldleg
       61 ntlgplaama vaealkekgs plkralwkdm ftgrlraeip kaleylgtal ctagtqltel
      121 dlsdnafgpv gaegiatfls ssscytlril klhntglgtt ggkilakall dcydnsskag
      181 tpplalkvfv agrnrledeg akalafvfqk ltsleevvmp qngiyhhgit aianglstns
      241 glkilnlndn tvgfkgaqal akvlpnfqnl eqlnlgdcll ktrgslilae alavkgnyps
      301 laevnlsyne irtkgansia hamadkkhli alqldgnafg kegrtllrns ltvskriesl
      361 gslsndesds eeendeenvd deeenedeee dddedddddd dddddddede dedeseiesk
      421 enedtvtngn etsnnvarvk vsiteflksp tgerllllqd nvvqgfidha knssknvdts
      481 pqlrfieeyt rivmkvsalc tsgyidvrlr aqnltdilys klcsfatend qisvwnnall
      541 vnlglikaed kcngkinwnl egcfkaleqv tqkdyfleei rttlkiflek stksrkakva
      601 dslqeskdsl kavlnhtqna gsqpg
//
LOCUS       XP_034183976             591 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183976
VERSION     XP_034183976.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328085.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183976.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="sugar baby transporter isoform X1"
                     /calculated_mol_wt=66296
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..591
                     /gene="Sugb"
                     /coded_by="XM_034328085.2:415..2190"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnhl vnyanpddai lltmnrelqt y
//
LOCUS       XP_034183921             591 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183921
VERSION     XP_034183921.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328030.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183921.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="sugar baby transporter isoform X1"
                     /calculated_mol_wt=66296
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..591
                     /gene="Sugb"
                     /coded_by="XM_034328030.2:184..1959"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnhl vnyanpddai lltmnrelqt y
//
LOCUS       XP_034183929             591 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183929
VERSION     XP_034183929.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328038.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183929.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="sugar baby transporter isoform X1"
                     /calculated_mol_wt=66296
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..591
                     /gene="Sugb"
                     /coded_by="XM_034328038.2:510..2285"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnhl vnyanpddai lltmnrelqt y
//
LOCUS       XP_034183937             591 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183937
VERSION     XP_034183937.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328046.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183937.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="sugar baby transporter isoform X1"
                     /calculated_mol_wt=66296
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..591
                     /gene="Sugb"
                     /coded_by="XM_034328046.2:468..2243"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnhl vnyanpddai lltmnrelqt y
//
LOCUS       XP_034183947             591 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183947
VERSION     XP_034183947.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328056.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183947.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="sugar baby transporter isoform X1"
                     /calculated_mol_wt=66296
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..591
                     /gene="Sugb"
                     /coded_by="XM_034328056.2:168..1943"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnhl vnyanpddai lltmnrelqt y
//
LOCUS       XP_034183956             591 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183956
VERSION     XP_034183956.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328065.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183956.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="sugar baby transporter isoform X1"
                     /calculated_mol_wt=66296
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..591
                     /gene="Sugb"
                     /coded_by="XM_034328065.2:459..2234"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnhl vnyanpddai lltmnrelqt y
//
LOCUS       XP_034183966             591 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183966
VERSION     XP_034183966.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034328075.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183966.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="sugar baby transporter isoform X1"
                     /calculated_mol_wt=66296
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..591
                     /gene="Sugb"
                     /coded_by="XM_034328075.2:444..2219"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnhl vnyanpddai lltmnrelqt y
//
LOCUS       XP_076546464             570 aa            linear   INV 03-SEP-2025
DEFINITION  sugar baby transporter isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546464
VERSION     XP_076546464.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690349.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="sugar baby transporter isoform X2"
                     /calculated_mol_wt=63894
     Region          11..551
                     /region_name="MFS_MFSD6"
                     /note="Major facilitator superfamily domain-containing
                     protein 6; cd17335"
                     /db_xref="CDD:340893"
     Site            order(19..20,23..24,27,59,266..267,269..271,274,293,
                     296..297,300,378,381..382,385..387,390,424,428,477..478,
                     482,486,507,510..511,514..515,518)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340893"
     CDS             1..570
                     /gene="Sugb"
                     /coded_by="XM_076690349.1:418..2130"
                     /db_xref="GeneID:117606048"
ORIGIN      
        1 mfeninrell pikahyflfn aatgpivpfl piiakqlgfs gilvgtiyti lpvsglvakp
       61 lfggladkfk ihkplfllfq avvaiamfti lfipeidrsa nvtlnclgea sleicsknef
      121 ssevihsvis ekmhtntsch vsckatkdiy telclhwkas efckllnsni sdtdtfdfnm
      181 rfdifhdihi gkciyihnft amfsndeayk phcanyvktf cktichnnpa fnqlleeaev
      241 sqnstqtssy qfqsflwati iswigmavvv siadaicfnl lgndrrkeyg kqkmwgsigf
      301 gifgisagyl vdvfsegrtq kdyscifyim limmifdiiv satlrkknpd csedepsifq
      361 elftifkegn vlafgwwcig agmctsviwn flfwytedla tgvqitwlkt lqglltgiqc
      421 fvgelpfnfi sgtvlrklgh vnvmslvlli yairfmayst vsnpwwflii evlhgpslgl
      481 cwptmvsygd kvtppgtkat iqgfigavfe gigvscgsli cgwlmdtygg vttmrvfsvg
      541 allwltcfwf illlvrklkv tplhlghnre
//
LOCUS       XP_034172810             546 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylserine lipase ABHD16A [Osmia lignaria lignaria].
ACCESSION   XP_034172810
VERSION     XP_034172810.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="phosphatidylserine lipase ABHD16A"
                     /calculated_mol_wt=61473
     Region          <282..411
                     /region_name="FrsA"
                     /note="Fermentation-respiration switch esterase FrsA,
                     DUF1100 family [Signal transduction mechanisms]; COG1073"
                     /db_xref="CDD:440691"
     CDS             1..546
                     /gene="LOC117600893"
                     /coded_by="XM_034316919.2:218..1858"
                     /db_xref="GeneID:117600893"
ORIGIN      
        1 msfirtlwkc tfsprlfkiy eitwigrlvd ksyepnsier wgdqivicfa aiwsislyti
       61 plvaiffyqr nsslsdniys lsklvvgaga ifiasliars csrannpvyl kflktlddan
      121 ahyntetkqe lhkydfefwa wpvdfkvpdi vgdkhqeklt lekiavssgr vkrqsgkefv
      181 ftlpckllsy ivahtfgikm iypgsvsvin wafrstllkg rmdlikrgge ryklltadnn
      241 eidtmfidqr nktsngnvlv itcegncgfy esgiistpln kgysilgwnh pgfgsstgap
      301 yplqeenaid cvmrfaidrl gfdeeqiily gwsiggypat waamnypsiq slvldatfdd
      361 ilplaimtms ssleglirni irdyfnlnia eqlnryngtv llirrtddev vctpsntlsg
      421 nrgnmlltkl lmrryphlls etaecavllv rflsadvaar kslietvkvd ekqcleliae
      481 diennggivs ypstlgqdcd skikqqlilf latmymkdqp sshctplavd lfhpgwdpas
      541 amsikh
//
LOCUS       XP_034172826             510 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600894 [Osmia lignaria lignaria].
ACCESSION   XP_034172826
VERSION     XP_034172826.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316935.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172826.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="uncharacterized protein LOC117600894"
                     /calculated_mol_wt=59105
     Region          131..170
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          182..219
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          206..243
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          230..267
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          287..327
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          336..375
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          384..423
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     CDS             1..510
                     /gene="LOC117600894"
                     /coded_by="XM_034316935.2:53..1585"
                     /db_xref="GeneID:117600894"
ORIGIN      
        1 mrirpgcaaa lavllqvalv askaldqnql qqqksqqqip ygtvaqkrwg gdagghaggd
       61 vgtgyggdfg ghggdsggyg gdigggfghs ggggdigggf ghsgggdigg gdaggagdeh
      121 hddhhhehgy wkkkliwkpg wkkiwkpakk qiwkpawkki wkpiwvptqk avwkdiqvpa
      181 wkkiwkpvwk eiqvpvwkei qvpawkkiwk pvwkpikvpa wkeiqvpawk kiwkpvwkei
      241 qvpawkeiqv pawkkiwipe wvkigipgeh yhgtdhhgwe ytshdlwkkk liwkplwkky
      301 wkpakkqiwv pdkklewvea wkqiwkpakk qiwvddkkli wkeewkqiwk pskkliwvpd
      361 kklewkeawk qiwktdkkle wvpdkklswk eawkqiwvpa wkeiwvpawk kiwkpvwise
      421 wfpaedhhph hhgwedrkds qaqgsqvvpy sseasakqna qgqqqqrqvd enkvrwdrss
      481 kteapslsqd lvpppaptnq ssanfafpkr
//
LOCUS       XP_034172840             494 aa            linear   INV 03-SEP-2025
DEFINITION  methyl farnesoate epoxidase [Osmia lignaria lignaria].
ACCESSION   XP_034172840
VERSION     XP_034172840.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316949.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172840.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="methyl farnesoate epoxidase"
                     /calculated_mol_wt=56887
     Region          65..487
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(123,127,134,182,298..299,302..303,306..307,310,361,
                     371,373,396,432..434,438..442,445..446)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(208..209,298,301..302,306,369..372)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..494
                     /gene="LOC117600899"
                     /coded_by="XM_034316949.2:20..1504"
                     /db_xref="GeneID:117600899"
ORIGIN      
        1 mfyaviclll vlfcifcvyd cfkpqnyppg pkwmpligcf ftfrqlklkn gyvylafhel
       61 mksygpilgl klgtqkvvvi sthdlvkkvl lqdefngrpd gfffrvrafg kkrgilfaeg
      121 ptwaqcrrft mrhlrafgfg qsimekqlii eaenfvdylr rasakgpvpm htafdvavln
      181 slwsmlaghr fdynneklme ildavhgafr lmdtmggivs hmpflrfiip elsgynelmr
      241 ilrklwgfld eeitihekql sdnepqdlie aflleissnr iiddsifdre nllivcldlf
      301 lagsktttdt laatllflsl hsdwikilqe eldnvvgrsr spalkdsssl pmmesflaev
      361 qrylnlapfg lphktmkdvn lngyhipkdt milldfhsvh ndkaywdhpe efrpqrfldd
      421 tgrfcpnsas mifslgkrrc pgemlarstl flfftyvvyy fdieispdhg kpdpngydgf
      481 tispkpyhlk ltlr
//
LOCUS       XP_034172851             487 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 305a1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172851
VERSION     XP_034172851.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316960.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172851.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..487
                     /product="putative cytochrome P450 305a1 isoform X1"
                     /calculated_mol_wt=55680
     Region          65..481
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(122,126,133,178,292..293,296..297,300..301,304,355,
                     365,367,390,425..427,431..435,438..439)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(203..204,292,295..296,300,363..366)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..487
                     /gene="LOC117600907"
                     /coded_by="XM_034316960.2:805..2268"
                     /db_xref="GeneID:117600907"
ORIGIN      
        1 mfitvvliil ililvvvkhk vknqppgpfp wpvignqfll krlthklgaq hfafielskr
       61 ynshlislgs gankvlvvsg sescmmevlk seefegrpwn afiemrnmgk kqgitmndgd
      121 ewreirgwtm rtmrsfgfgk qnmldmikne lniilekmkk ggvqrlkpmi vpavinvlwm
      181 lttgkpfgdd rklqyftell errarafdmn ggflaafpwl ryvapeisgy nllvtlnnel
      241 kaflmdiine hkrqyspenn ddlidlflhe mysengsssv fnedqlvmvl idfflagctt
      301 tatsmdflfl immlhqdvqr klqqeidsvi prdefpdlkh kiklpyteav itesqrlwpp
      361 fpiigprrcl rdtnlggyki pkdstillnm ysvhvdpely tdphtfnper fikdgvfepn
      421 vnslsfgkgr rrcpgemlak samfllftgi mqkytllpvp gkgptslein pgltispkpy
      481 qslvvpr
//
LOCUS       XP_034172894             403 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 305a1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172894
VERSION     XP_034172894.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172894.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="putative cytochrome P450 305a1 isoform X2"
                     /calculated_mol_wt=46396
     Region          3..397
                     /region_name="cytochrome_P450"
                     /note="cytochrome P450 (CYP) superfamily; cl41757"
                     /db_xref="CDD:477761"
     Site            order(38,42,49,94,208..209,212..213,216..217,220,271,281,
                     283,306,341..343,347..351,354..355)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410651"
     Site            order(119..120,208,211..212,216,279..282)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410651"
     CDS             1..403
                     /gene="LOC117600907"
                     /coded_by="XM_034317003.2:586..1797"
                     /db_xref="GeneID:117600907"
ORIGIN      
        1 mmevlkseef egrpwnafie mrnmgkkqgi tmndgdewre irgwtmrtmr sfgfgkqnml
       61 dmiknelnii lekmkkggvq rlkpmivpav invlwmlttg kpfgddrklq yftellerra
      121 rafdmnggfl aafpwlryva peisgynllv tlnnelkafl mdiinehkrq yspennddli
      181 dlflhemyse ngsssvfned qlvmvlidff lagctttats mdflflimml hqdvqrklqq
      241 eidsviprde fpdlkhkikl pyteavites qrlwppfpii gprrclrdtn lggykipkds
      301 tillnmysvh vdpelytdph tfnperfikd gvfepnvnsl sfgkgrrrcp gemlaksamf
      361 llftgimqky tllpvpgkgp tsleinpglt ispkpyqslv vpr
//
LOCUS       XP_076546465             466 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600935 [Osmia lignaria lignaria].
ACCESSION   XP_076546465
VERSION     XP_076546465.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690350.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="uncharacterized protein LOC117600935"
                     /calculated_mol_wt=52147
     Region          31..160
                     /region_name="Mucin-like"
                     /note="pfam16058"
                     /db_xref="CDD:464997"
     Region          209..249
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          260..297
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          284..321
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          341..381
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          366..405
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          414..453
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     CDS             1..466
                     /gene="LOC117600935"
                     /coded_by="XM_076690350.1:1977..3377"
                     /db_xref="GeneID:117600935"
ORIGIN      
        1 mrssllllgc fflvstlgdp irtkkeapls pppsqygpps asygvpnvgs synapsdsyg
       61 aplpsssyga psgssshggs fgpsssygap sgpsssygap sgpssshgap sssygapsss
      121 ygapsssyga psgpsssyga pssfdhgssf ggdqgyssgg sfgdhhsggg hggdhgsggg
      181 vsssygppsq sygppaqsyg ppaqpqgrwe kkltwkpewk qiwkteqklt wkqewkkvqv
      241 pvwkeiqvpa wkevkvsawk kvqkpvwkei qvpawkevqv pawkkvwkpv wkeiqvpiwk
      301 evqvpdwkki wvpewikvgi pgeqyvgkdq hgwqytshdl wkkkliwkpv wkkiwrtekk
      361 qawasdkkle wkaewkqiwk tekkqvwvsd kklvwkeesv qvwvpekkqi wvtqkkqvwk
      421 dewkskwvpv wkdvqvpawk kiwkpvwekv wvpaephhde hhhgyk
//
LOCUS       XP_034172922             466 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600935 [Osmia lignaria lignaria].
ACCESSION   XP_034172922
VERSION     XP_034172922.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317031.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172922.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="uncharacterized protein LOC117600935"
                     /calculated_mol_wt=52147
     Region          31..160
                     /region_name="Mucin-like"
                     /note="pfam16058"
                     /db_xref="CDD:464997"
     Region          209..249
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          260..297
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          284..321
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          341..381
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          366..405
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          414..453
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     CDS             1..466
                     /gene="LOC117600935"
                     /coded_by="XM_034317031.2:1829..3229"
                     /db_xref="GeneID:117600935"
ORIGIN      
        1 mrssllllgc fflvstlgdp irtkkeapls pppsqygpps asygvpnvgs synapsdsyg
       61 aplpsssyga psgssshggs fgpsssygap sgpsssygap sgpssshgap sssygapsss
      121 ygapsssyga psgpsssyga pssfdhgssf ggdqgyssgg sfgdhhsggg hggdhgsggg
      181 vsssygppsq sygppaqsyg ppaqpqgrwe kkltwkpewk qiwkteqklt wkqewkkvqv
      241 pvwkeiqvpa wkevkvsawk kvqkpvwkei qvpawkevqv pawkkvwkpv wkeiqvpiwk
      301 evqvpdwkki wvpewikvgi pgeqyvgkdq hgwqytshdl wkkkliwkpv wkkiwrtekk
      361 qawasdkkle wkaewkqiwk tekkqvwvsd kklvwkeesv qvwvpekkqi wvtqkkqvwk
      421 dewkskwvpv wkdvqvpawk kiwkpvwekv wvpaephhde hhhgyk
//
LOCUS       XP_076546466             466 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600935 [Osmia lignaria lignaria].
ACCESSION   XP_076546466
VERSION     XP_076546466.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690351.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="uncharacterized protein LOC117600935"
                     /calculated_mol_wt=52147
     Region          31..160
                     /region_name="Mucin-like"
                     /note="pfam16058"
                     /db_xref="CDD:464997"
     Region          209..249
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          260..297
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          284..321
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          341..381
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          366..405
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          414..453
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     CDS             1..466
                     /gene="LOC117600935"
                     /coded_by="XM_076690351.1:1735..3135"
                     /db_xref="GeneID:117600935"
ORIGIN      
        1 mrssllllgc fflvstlgdp irtkkeapls pppsqygpps asygvpnvgs synapsdsyg
       61 aplpsssyga psgssshggs fgpsssygap sgpsssygap sgpssshgap sssygapsss
      121 ygapsssyga psgpsssyga pssfdhgssf ggdqgyssgg sfgdhhsggg hggdhgsggg
      181 vsssygppsq sygppaqsyg ppaqpqgrwe kkltwkpewk qiwkteqklt wkqewkkvqv
      241 pvwkeiqvpa wkevkvsawk kvqkpvwkei qvpawkevqv pawkkvwkpv wkeiqvpiwk
      301 evqvpdwkki wvpewikvgi pgeqyvgkdq hgwqytshdl wkkkliwkpv wkkiwrtekk
      361 qawasdkkle wkaewkqiwk tekkqvwvsd kklvwkeesv qvwvpekkqi wvtqkkqvwk
      421 dewkskwvpv wkdvqvpawk kiwkpvwekv wvpaephhde hhhgyk
//
LOCUS       XP_076546467             466 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600935 [Osmia lignaria lignaria].
ACCESSION   XP_076546467
VERSION     XP_076546467.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690352.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="uncharacterized protein LOC117600935"
                     /calculated_mol_wt=52147
     Region          31..160
                     /region_name="Mucin-like"
                     /note="pfam16058"
                     /db_xref="CDD:464997"
     Region          209..249
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          260..297
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          284..321
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          341..381
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          366..405
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          414..453
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     CDS             1..466
                     /gene="LOC117600935"
                     /coded_by="XM_076690352.1:1855..3255"
                     /db_xref="GeneID:117600935"
ORIGIN      
        1 mrssllllgc fflvstlgdp irtkkeapls pppsqygpps asygvpnvgs synapsdsyg
       61 aplpsssyga psgssshggs fgpsssygap sgpsssygap sgpssshgap sssygapsss
      121 ygapsssyga psgpsssyga pssfdhgssf ggdqgyssgg sfgdhhsggg hggdhgsggg
      181 vsssygppsq sygppaqsyg ppaqpqgrwe kkltwkpewk qiwkteqklt wkqewkkvqv
      241 pvwkeiqvpa wkevkvsawk kvqkpvwkei qvpawkevqv pawkkvwkpv wkeiqvpiwk
      301 evqvpdwkki wvpewikvgi pgeqyvgkdq hgwqytshdl wkkkliwkpv wkkiwrtekk
      361 qawasdkkle wkaewkqiwk tekkqvwvsd kklvwkeesv qvwvpekkqi wvtqkkqvwk
      421 dewkskwvpv wkdvqvpawk kiwkpvwekv wvpaephhde hhhgyk
//
LOCUS       XP_076546468             466 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600935 [Osmia lignaria lignaria].
ACCESSION   XP_076546468
VERSION     XP_076546468.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690353.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="uncharacterized protein LOC117600935"
                     /calculated_mol_wt=52147
     Region          31..160
                     /region_name="Mucin-like"
                     /note="pfam16058"
                     /db_xref="CDD:464997"
     Region          209..249
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          260..297
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          284..321
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          341..381
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          366..405
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          414..453
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     CDS             1..466
                     /gene="LOC117600935"
                     /coded_by="XM_076690353.1:1729..3129"
                     /db_xref="GeneID:117600935"
ORIGIN      
        1 mrssllllgc fflvstlgdp irtkkeapls pppsqygpps asygvpnvgs synapsdsyg
       61 aplpsssyga psgssshggs fgpsssygap sgpsssygap sgpssshgap sssygapsss
      121 ygapsssyga psgpsssyga pssfdhgssf ggdqgyssgg sfgdhhsggg hggdhgsggg
      181 vsssygppsq sygppaqsyg ppaqpqgrwe kkltwkpewk qiwkteqklt wkqewkkvqv
      241 pvwkeiqvpa wkevkvsawk kvqkpvwkei qvpawkevqv pawkkvwkpv wkeiqvpiwk
      301 evqvpdwkki wvpewikvgi pgeqyvgkdq hgwqytshdl wkkkliwkpv wkkiwrtekk
      361 qawasdkkle wkaewkqiwk tekkqvwvsd kklvwkeesv qvwvpekkqi wvtqkkqvwk
      421 dewkskwvpv wkdvqvpawk kiwkpvwekv wvpaephhde hhhgyk
//
LOCUS       XP_076546469             466 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600935 [Osmia lignaria lignaria].
ACCESSION   XP_076546469
VERSION     XP_076546469.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690354.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..466
                     /product="uncharacterized protein LOC117600935"
                     /calculated_mol_wt=52147
     Region          31..160
                     /region_name="Mucin-like"
                     /note="pfam16058"
                     /db_xref="CDD:464997"
     Region          209..249
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          260..297
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          284..321
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          341..381
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          366..405
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     Region          414..453
                     /region_name="DUF4816"
                     /note="Domain of unknown function (DUF4816); pfam16086"
                     /db_xref="CDD:465010"
     CDS             1..466
                     /gene="LOC117600935"
                     /coded_by="XM_076690354.1:1741..3141"
                     /db_xref="GeneID:117600935"
ORIGIN      
        1 mrssllllgc fflvstlgdp irtkkeapls pppsqygpps asygvpnvgs synapsdsyg
       61 aplpsssyga psgssshggs fgpsssygap sgpsssygap sgpssshgap sssygapsss
      121 ygapsssyga psgpsssyga pssfdhgssf ggdqgyssgg sfgdhhsggg hggdhgsggg
      181 vsssygppsq sygppaqsyg ppaqpqgrwe kkltwkpewk qiwkteqklt wkqewkkvqv
      241 pvwkeiqvpa wkevkvsawk kvqkpvwkei qvpawkevqv pawkkvwkpv wkeiqvpiwk
      301 evqvpdwkki wvpewikvgi pgeqyvgkdq hgwqytshdl wkkkliwkpv wkkiwrtekk
      361 qawasdkkle wkaewkqiwk tekkqvwvsd kklvwkeesv qvwvpekkqi wvtqkkqvwk
      421 dewkskwvpv wkdvqvpawk kiwkpvwekv wvpaephhde hhhgyk
//
LOCUS       XP_034172956             433 aa            linear   INV 03-SEP-2025
DEFINITION  retinoid-inducible serine carboxypeptidase [Osmia lignaria
            lignaria].
ACCESSION   XP_034172956
VERSION     XP_034172956.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317065.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172956.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="retinoid-inducible serine carboxypeptidase"
                     /calculated_mol_wt=48282
     Region          25..431
                     /region_name="Peptidase_S10"
                     /note="Serine carboxypeptidase; pfam00450"
                     /db_xref="CDD:459815"
     CDS             1..433
                     /gene="LOC117600948"
                     /coded_by="XM_034317065.2:104..1405"
                     /db_xref="GeneID:117600948"
ORIGIN      
        1 mrflililva lcftskslak kgfgpgeqew gyvtvrpnan mfwwlyytta nvqsyyekpl
       61 viwlqggpgs ssttygnfee lgpldvdlkp rnytwvkdyn vlfidnpvgt gysyttspsa
      121 yattnaqian dllecikgfi kelprfsnvp tyittesygg kmgaefaltw ykaqqagsiq
      181 snlkgvalgd awispidsvm twapflqatg midragyeki dqaaqmtkra vergtwktat
      241 vlwantqnai fdvtgnidfy niltkmtsty tripniekfl tdpmsvsngs vfgsnslkel
      301 mngpvkedld lpvdhgkqsn tvfanlsedf mkpvidkveq llnqtdlkvv vltghmdliv
      361 dtpgtinwve klkwknataw nnakrvpmvv nniiegysks qgnfalywvd raghmvpkdn
      421 pngmaqilkd fan
//
LOCUS       XP_034173010             356 aa            linear   INV 03-SEP-2025
DEFINITION  GDP-mannose 4,6 dehydratase [Osmia lignaria lignaria].
ACCESSION   XP_034173010
VERSION     XP_034173010.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317119.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..356
                     /product="GDP-mannose 4,6 dehydratase"
                     /calculated_mol_wt=40207
     Region          8..351
                     /region_name="Gmd"
                     /note="GDP-D-mannose dehydratase [Cell
                     wall/membrane/envelope biogenesis]; COG1089"
                     /db_xref="CDD:440706"
     CDS             1..356
                     /gene="Gmd"
                     /coded_by="XM_034317119.2:89..1159"
                     /db_xref="GeneID:117600974"
ORIGIN      
        1 matgderrva litgitgqdg sylaefllek gydvhgiirr assfntariq hlyadpkchr
       61 qgkmklhygd mtdssslvkv issvqpteiy nlaaqshvmv sfevseytae vdavgtvrll
      121 dairtcglek svkfyhasts elygrvtqvp qnektpfypr spyacaklys fwivvnyrea
      181 ydmfacngil fnhesprrge nfvtrkvtrs iakiqlglqd flelgnldak rdwghakdyv
      241 eamwlmlqqs npddyviatg ethsvrefve dafhyvgrti kwqgegvnet gqdaetgkvl
      301 vkvnpkyfrp tevdvllgda tkakekigwk ptvtfedlvk dmmdsdlelm rknpna
//
LOCUS       XP_034173066             248 aa            linear   INV 03-SEP-2025
DEFINITION  forkhead box protein I1-ema isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034173066
VERSION     XP_034173066.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317175.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="forkhead box protein I1-ema isoform X1"
                     /calculated_mol_wt=28975
     Region          73..171
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(79,97..98,101,120..121,123..124,127,134,152..154,
                     156)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410788"
     CDS             1..248
                     /gene="LOC117601002"
                     /coded_by="XM_034317175.2:1817..2563"
                     /db_xref="GeneID:117601002"
ORIGIN      
        1 mdsnvqdhpc svskaygcdl wrsyelwnmd evfptmcplp syynlvpsmv nsrnvplswg
       61 lplmrykpqp ryekppysyi aliamainss pkqrltlsgi yrfimdrfpy yrenrqgwqn
      121 sirhnlslnd cfvkiprdkv vgngneedqa gkgsywtldp sasgmfehgn yrrrrmrrqr
      181 givqgkqeqd raivsvsskl ltnfkhqern ekatemietk dskdnsrnmk kysesmftie
      241 nilrkstn
//
LOCUS       XP_034173075             203 aa            linear   INV 03-SEP-2025
DEFINITION  fork head domain-containing protein L1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173075
VERSION     XP_034173075.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317184.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..203
                     /product="fork head domain-containing protein L1 isoform
                     X2"
                     /calculated_mol_wt=23715
     Region          73..171
                     /region_name="FH_FOX"
                     /note="Forkhead (FH) domain found in Forkhead box (FOX)
                     family of transcription factors and similar proteins;
                     cl00061"
                     /db_xref="CDD:469596"
     Site            order(79,97..98,101,120..121,123..124,127,134,152..154,
                     156)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410788"
     CDS             1..203
                     /gene="LOC117601002"
                     /coded_by="XM_034317184.2:1817..2428"
                     /db_xref="GeneID:117601002"
ORIGIN      
        1 mdsnvqdhpc svskaygcdl wrsyelwnmd evfptmcplp syynlvpsmv nsrnvplswg
       61 lplmrykpqp ryekppysyi aliamainss pkqrltlsgi yrfimdrfpy yrenrqgwqn
      121 sirhnlslnd cfvkiprdkv vgngneedqa gkgsywtldp sasgmfehgn yrrrrmrrqr
      181 givqgiieaf dqfqaprkkr esn
//
LOCUS       XP_034173088             236 aa            linear   INV 03-SEP-2025
DEFINITION  proteasome beta6 subunit [Osmia lignaria lignaria].
ACCESSION   XP_034173088
VERSION     XP_034173088.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317197.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="proteasome beta6 subunit"
                     /calculated_mol_wt=25992
     Region          25..236
                     /region_name="proteasome_beta_type_1"
                     /note="proteasome beta type-1 subunit. The 20S proteasome,
                     multisubunit proteolytic complex, is the central enzyme of
                     nonlysosomal protein degradation in both the cytosol and
                     nucleus. It is composed of 28 subunits arranged as four
                     homoheptameric rings that...; cd03757"
                     /db_xref="CDD:239726"
     Site            order(33,49,51,65,163,209,212..213)
                     /site_type="active"
                     /db_xref="CDD:239726"
     Site            order(51,54,56..61,81,83,98,110,113..114,116..117,120,123,
                     148,150,152..155,158,166..167,169..170,173..175,200,
                     207..210,213..214)
                     /site_type="other"
                     /note="beta subunit interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239726"
     CDS             1..236
                     /gene="Prosbeta6"
                     /coded_by="XM_034317197.2:141..851"
                     /db_xref="GeneID:117601006"
ORIGIN      
        1 mtfisenpgr slpdyetqaa kmksfspyad nggsvialag defcviaadt rlstgfsiyt
       61 reqqklfpls nktilgcsgc wcdtltltrl lsarmqmyeh ehqkemptpa vaqmlstmly
      121 ykrffpyyvs nilggldekg ngcvysydpi ghcevtkyra sgsagallqp lldnqvgykn
      181 qegvepvplt eeraiaiikd vftsaaerdi ytgdsitikv itkdgvkdls fglrkd
//
LOCUS       XP_034173101             147 aa            linear   INV 03-SEP-2025
DEFINITION  multiprotein bridging factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034173101
VERSION     XP_034173101.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317210.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..147
                     /product="multiprotein bridging factor 1"
                     /calculated_mol_wt=16124
     Region          3..70
                     /region_name="MBF1"
                     /note="Multiprotein bridging factor 1; pfam08523"
                     /db_xref="CDD:462508"
     Region          72..131
                     /region_name="aMBF1"
                     /note="Archaeal ribosome-binding protein aMBF1, putative
                     translation factor, contains Zn-ribbon and HTH domains
                     [Translation, ribosomal structure and biogenesis];
                     COG1813"
                     /db_xref="CDD:441418"
     CDS             1..147
                     /gene="mbf1"
                     /coded_by="XM_034317210.2:276..719"
                     /db_xref="GeneID:117601012"
ORIGIN      
        1 msdwdtapit lrkrppkasv lkseqavnaa rrqgfvietq akwgggtnrq hvatkntakl
       61 dreteelkhd kipldlgkli qqgrqnkgls qkdlatkvne kaqvindyea grgipnqmvi
      121 gkiervlgik lrgkdrgkpl tapgakk
//
LOCUS       XP_076546470             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601025 [Osmia lignaria lignaria].
ACCESSION   XP_076546470
VERSION     XP_076546470.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690355.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117601025"
                     /calculated_mol_wt=13826
     CDS             1..116
                     /gene="LOC117601025"
                     /coded_by="XM_076690355.1:1501..1851"
                     /db_xref="GeneID:117601025"
ORIGIN      
        1 mkrkelflrq ekgtkvsvqn sierylknfv vvekrnsmer rsltsselfi drinsfqprr
       61 iyvhipeswh rneavpvlsv lakwhdyinv tktekqtdss sffreftmnl tfllet
//
LOCUS       XP_076546471             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601025 [Osmia lignaria lignaria].
ACCESSION   XP_076546471
VERSION     XP_076546471.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690356.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117601025"
                     /calculated_mol_wt=13826
     CDS             1..116
                     /gene="LOC117601025"
                     /coded_by="XM_076690356.1:1419..1769"
                     /db_xref="GeneID:117601025"
ORIGIN      
        1 mkrkelflrq ekgtkvsvqn sierylknfv vvekrnsmer rsltsselfi drinsfqprr
       61 iyvhipeswh rneavpvlsv lakwhdyinv tktekqtdss sffreftmnl tfllet
//
LOCUS       XP_076546472             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601025 [Osmia lignaria lignaria].
ACCESSION   XP_076546472
VERSION     XP_076546472.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690357.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117601025"
                     /calculated_mol_wt=13826
     CDS             1..116
                     /gene="LOC117601025"
                     /coded_by="XM_076690357.1:1519..1869"
                     /db_xref="GeneID:117601025"
ORIGIN      
        1 mkrkelflrq ekgtkvsvqn sierylknfv vvekrnsmer rsltsselfi drinsfqprr
       61 iyvhipeswh rneavpvlsv lakwhdyinv tktekqtdss sffreftmnl tfllet
//
LOCUS       XP_076546473             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117601025 [Osmia lignaria lignaria].
ACCESSION   XP_076546473
VERSION     XP_076546473.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690358.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117601025"
                     /calculated_mol_wt=13826
     CDS             1..116
                     /gene="LOC117601025"
                     /coded_by="XM_076690358.1:1513..1863"
                     /db_xref="GeneID:117601025"
ORIGIN      
        1 mkrkelflrq ekgtkvsvqn sierylknfv vvekrnsmer rsltsselfi drinsfqprr
       61 iyvhipeswh rneavpvlsv lakwhdyinv tktekqtdss sffreftmnl tfllet
//
LOCUS       XP_034172664            2772 aa            linear   INV 03-SEP-2025
DEFINITION  small subunit processome component 20 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034172664
VERSION     XP_034172664.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172664.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2772
                     /product="small subunit processome component 20 homolog"
                     /calculated_mol_wt=319453
     Region          889..1529
                     /region_name="DRIM"
                     /note="Down-regulated in metastasis; pfam07539"
                     /db_xref="CDD:462198"
     Region          1811..2027
                     /region_name="DUF6700"
                     /note="Domain of unknown function (DUF6700); pfam20416"
                     /db_xref="CDD:466565"
     CDS             1..2772
                     /gene="LOC117600841"
                     /coded_by="XM_034316773.2:133..8451"
                     /db_xref="GeneID:117600841"
ORIGIN      
        1 mknkptrhke tntfqfktfs erisevdidv fhrvahrnee neeietyfhq tlqkwnflnl
       61 tegycnfkre vrdivtltql inqkeyvvdk lieylerrdi sflqpilelv valskdlqke
      121 fyeyfpkflt vmidllrtkd adqieyafts laylfkflwr yliknvktvf dlllplladt
      181 qpayvnnfaa esfafvvrki kdkesflklv lnllednpkg ipgcgkllfe vihglpgqfh
      241 scteqmlsly fsalnyetvn qnlafrvlke iitcivqnih pqkchvfwsv vlnnidvaie
      301 ntkdfrsine rekslilimr llhpvidykn grlvidpvpl mkklvqalkm fenqsdalqe
      361 iikvavaill aadvklmqet ssqillqims vkdinllyea vnnlmhyssf etlilphiir
      421 hsisttfndd tlklftniie tkvplcingm kldkwtkyil dirgaetdsi nyllkeleal
      481 ldqnistnal rmliilphlk plseefnevl kkgilslykg ilnhdstesn liklclpfll
      541 aleaaihile petlysfmet ndikilnlis kypdnrfiln atdlcityfs tteyrekymn
      601 qtvfdilnss iasnlsspfg evrlivthly slfsdvkemt ssttnestek samehmylae
      661 cesitvqsfr dkllhlqals fennvvsnld pkyykfplqy llgnlyinfs llwepvnkii
      721 atyadkkced fwptflaelk reqqaitdyk pvfecetisk ieiaiqksnd kpdyanhkvl
      781 swkcmtnfaq fcemknrdit gmfidfvndn ffksnsedak ccsilkkqel nstdnnmeid
      841 dddddddesd neadeneekk eetkeptpfi stdskqrskf tvknnckikl liaqlevfek
      901 ianprslyre aeiqriyldl lssrnpdiqr gafnclltyk ykyllpykdn lynlineknl
      961 knevicfkid qesnmiqneh rdefipilmr ivyakmalkt gmrtggkvgg itrrklllrf
     1021 lggiqesemi iftkmafrpf kayisfesde kinlkeltrs ivntidlnnv ippkrlksav
     1081 nllgilieqf gskmakkllp cllglvlcil aavtgilqks dkvyagylps iknvrtscil
     1141 ilarffnhfe dyewseheld alfdvavfpw lqklpmegih sptpllklfm swsqnsryyp
     1201 lfikhreddk svsplpyimq lllgpkthas vinaildmie kmvtlqdygk tnendmeida
     1261 pfvplipvlt nllevnekal ssginygsai llphvvsile hiknklgksn krinrteliv
     1321 lsrisefvkd pnacdilvtl tlpilikkss aggaeettve littvinlik hvaqptvhir
     1381 ailpllgtvs viparkllle lyrtivesat edcreilikn ynlisalnaw drrwidqpdf
     1441 qkrldafsei nnavekneit lefgiaiihn cyyflknead lalrdysgqc lkllgsklak
     1501 ehrenisdrr ylmddtilll ikngitskne vvklqsiafl ghmaiecpev hpvlrdlsll
     1561 ankadpevdf fenmqhlqlh rrarallkfc tvaktlqkkf nprtltqfil pfasmylcne
     1621 sfihknsild aaietvgtvc kllpwyqyei ilkyyigklr gsaeyqkqlv riivimldsf
     1681 hfnlskykpk dgsletkdvk etlkneissd kkedvekeid enineeieek leetlksesl
     1741 dvieekdgte aqediipiie keiilspyga qrviftiske llpqlhrsii aktsresshk
     1801 fnkkkvaarn eeeelmrvpi alamvkllqk lpenildinl pgifmklctf lksrlesvrr
     1861 atreilqkim itlgpkylhy liremntllt rgfqvhvlaf tvqsvlvalk pyfqkfdink
     1921 nlqsilsvck vdlfgptgee kevlgivktv aeakstksfd ifhilaqyit esclvdlilp
     1981 lkevlvkths hktlqkvvec lrnvvlglad ntfipleqml tflygivses ipelmpekkd
     2041 kqltpkeaev marqkpdcfl ippepknkmg vkvtsktskn tnvhviiefg lkllhillkr
     2101 dkisggefes fldpfvplis dclksqhvkl stlglqclnw llkkdlpsih erisdicisi
     2161 fnilhkyaae glskgdnfdl vmagfkcmsv lvrdvkhyti neeqlkvlim yaeqdmydsd
     2221 rhatafgllk aiisrkmvlp eihnvmvkia tlsitsdleh irqqsrsvfy tylmeyplgk
     2281 llnkhilfyl tqlsyemqpg rlsalemlhs ivtgfptrtl imkadpifim agarlinddd
     2341 ptcrklcakc ikemvtrirh nkrsklfniv mewlrdtkim hralaaqlcg ifvtvekdaf
     2401 esrlkellpp llkqfhanid dneepgrfvk lrtneemael emqpnikdpe rmkdhhmfqv
     2461 lqlllkisan ctaflkndey keavrsfaey sqsllahpht wvrlaasqmi gfilaaldid
     2521 kiieflenpe kcetensymy sdpagtirsl tldliaqlqp dmmleeladq tvknlifiak
     2581 ilksvktvtt vtvdedkkat dtdknqlsls wlvrrlrrsv nieitkapks itvrtaffkw
     2641 vagivvtipm eylntvlfni mspivreist teekntslrr lakeaaamik krldneeyir
     2701 llsriqqqld ikkaerrkvr tqqyvtdpel aakrkiarqq kkkearkrks dtvrgkkgak
     2761 krlkkeidld ii
//
LOCUS       XP_034172678             887 aa            linear   INV 03-SEP-2025
DEFINITION  kinesin-like protein KIF18A [Osmia lignaria lignaria].
ACCESSION   XP_034172678
VERSION     XP_034172678.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316787.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172678.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..887
                     /product="kinesin-like protein KIF18A"
                     /calculated_mol_wt=100749
     Region          51..390
                     /region_name="KISc_KIP3_like"
                     /note="Kinesin motor domain, KIP3-like subgroup; cd01370"
                     /db_xref="CDD:276821"
     Site            order(59,154,157,159..162,296)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276821"
     Site            order(343,346,349)
                     /site_type="other"
                     /note="microtubule interaction site [polypeptide binding]"
                     /db_xref="CDD:276821"
     Region          <543..630
                     /region_name="S_2TMBeta"
                     /note="SMODS-associating 2TM, beta-strand rich effector
                     domain; pfam18153"
                     /db_xref="CDD:407984"
     CDS             1..887
                     /gene="LOC117600846"
                     /coded_by="XM_034316787.2:255..2918"
                     /db_xref="GeneID:117600846"
ORIGIN      
        1 mvfnkkdlak afspnkakkl akkrlsnngt rpstsgstal siqtesgsqt sikvivrvrp
       61 qndrelqsnc rsiikliddk mlifdpkeee npffyhgvaq kgrdllkkqn kelefifdkv
      121 fdmssnntdv fegstkdlii slldgyncsv faygatgagk thtmlgnnqd pgityrtvae
      181 lffqiekqge hrefnlgvty leiynenvqd llhksgplhl rddgrhgvii aglkvitihs
      241 aeelltllak gnknrtqhpt daneessrsh avfqvyinit nkldgqvrqv klsmidlags
      301 erasatgckg arfkeganin ksllalgnci nnladgakhi tyrdskltrl lkdslggncq
      361 tvmianiaps sfsyedtynt lryanrakki kthikkniis cemhvtayik mveeqkkein
      421 ylkqkllvfe sgstpmlpel kdskvdeeid kcaltisnkl iellhkkrvl nekvlslesa
      481 dkilccriqy kkaaderlqn ltaavdastp eeqntsgksr vnkslhyfer qrdslkiqie
      541 aaweelcsie telqklnidi kskkfgkled mialqtceie ksrlrqqyeh akkvnnlqqc
      601 emqshysmik mmsttlqnyy nmmrgygtmt dtmkeefkql vkllegvrni kwsdmetinh
      661 eecfysltcl sipnlidpln nkvpictlyp iddqnqnqnc tkdtlnttfn asttekdnvs
      721 dvvvndttvt lqtdrnisfn ektkgkrerk esieeeddnv nctekvkkrv lldknninii
      781 kmptkqarki fprdkdtsca igakekenkq hiqkshsitm sarsvailnk lkadklkknn
      841 ldkentdrsl kvvrekerrg lmtthpyqkp iskqkliaap pssrvpr
//
LOCUS       XP_034172715             829 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600857 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172715
VERSION     XP_034172715.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316824.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172715.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..829
                     /product="uncharacterized protein LOC117600857 isoform X1"
                     /calculated_mol_wt=93620
     Region          <184..248
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..829
                     /gene="LOC117600857"
                     /coded_by="XM_034316824.2:680..3169"
                     /db_xref="GeneID:117600857"
ORIGIN      
        1 mgldteltgi lrdvvqflec lrevklptsl ecirnnllir skdtltmfmv erlgasnspe
       61 pylnmnaarp kgllatlkte sselqeyvga eesrtqkqhq qhqdyyetlr tidavtneta
      121 sprdtdeqie ntlfdvytnl saaqaknkcq mygsldrreg kklfvfeqyr pcwvgliglh
      181 lliygsdrdn rpctvlpihg ymaraapnai prdqrrsest feifcpgsrt fqfvarnrkn
      241 meewiakice lggaekpdhk tsstkaaina svesdttrpd eqknsnckee ryqdvgslti
      301 dnvseeasil tnvvvsehid kettndsssk dtsrettnss sssslppllv stctivpaas
      361 silspppppp ppppppsspa rilntssfsq iprklpslpv hlspyeflde eeddiyhkie
      421 dfrdstrygn vgetetdnar skkepvtydd vranikgest sdheqtrkds knvarkslnl
      481 qnettyddtt ttntvskdtk kvavneqakf vsyddvenla larstkvrgt dktdestksp
      541 qkksfldrvr skkespkkne kkvkckpapa svstsllvnd qeplpiyydd vsdltnvqqm
      601 krfeeensey tcppaprpiy vkpptivgtv tdsqefyddv gtyrerhekd hdrdfckfhq
      661 pkkhnygrdq vptdndrsll ceniqdnehy ktpridaghp ksvlidqrde lyddiailad
      721 ftarqkeyfg kknseeitkl qvspekrswn rfvsgkkskt fdstvsetnn srvsngtedt
      781 tddfneqqss mrmntiqkli srmensfgka svrtassmll nktnvanna
//
LOCUS       XP_034172722             829 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600857 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172722
VERSION     XP_034172722.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172722.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..829
                     /product="uncharacterized protein LOC117600857 isoform X1"
                     /calculated_mol_wt=93620
     Region          <184..248
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..829
                     /gene="LOC117600857"
                     /coded_by="XM_034316831.2:954..3443"
                     /db_xref="GeneID:117600857"
ORIGIN      
        1 mgldteltgi lrdvvqflec lrevklptsl ecirnnllir skdtltmfmv erlgasnspe
       61 pylnmnaarp kgllatlkte sselqeyvga eesrtqkqhq qhqdyyetlr tidavtneta
      121 sprdtdeqie ntlfdvytnl saaqaknkcq mygsldrreg kklfvfeqyr pcwvgliglh
      181 lliygsdrdn rpctvlpihg ymaraapnai prdqrrsest feifcpgsrt fqfvarnrkn
      241 meewiakice lggaekpdhk tsstkaaina svesdttrpd eqknsnckee ryqdvgslti
      301 dnvseeasil tnvvvsehid kettndsssk dtsrettnss sssslppllv stctivpaas
      361 silspppppp ppppppsspa rilntssfsq iprklpslpv hlspyeflde eeddiyhkie
      421 dfrdstrygn vgetetdnar skkepvtydd vranikgest sdheqtrkds knvarkslnl
      481 qnettyddtt ttntvskdtk kvavneqakf vsyddvenla larstkvrgt dktdestksp
      541 qkksfldrvr skkespkkne kkvkckpapa svstsllvnd qeplpiyydd vsdltnvqqm
      601 krfeeensey tcppaprpiy vkpptivgtv tdsqefyddv gtyrerhekd hdrdfckfhq
      661 pkkhnygrdq vptdndrsll ceniqdnehy ktpridaghp ksvlidqrde lyddiailad
      721 ftarqkeyfg kknseeitkl qvspekrswn rfvsgkkskt fdstvsetnn srvsngtedt
      781 tddfneqqss mrmntiqkli srmensfgka svrtassmll nktnvanna
//
LOCUS       XP_034172726             829 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600857 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172726
VERSION     XP_034172726.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316835.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172726.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..829
                     /product="uncharacterized protein LOC117600857 isoform X1"
                     /calculated_mol_wt=93620
     Region          <184..248
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     CDS             1..829
                     /gene="LOC117600857"
                     /coded_by="XM_034316835.2:188..2677"
                     /db_xref="GeneID:117600857"
ORIGIN      
        1 mgldteltgi lrdvvqflec lrevklptsl ecirnnllir skdtltmfmv erlgasnspe
       61 pylnmnaarp kgllatlkte sselqeyvga eesrtqkqhq qhqdyyetlr tidavtneta
      121 sprdtdeqie ntlfdvytnl saaqaknkcq mygsldrreg kklfvfeqyr pcwvgliglh
      181 lliygsdrdn rpctvlpihg ymaraapnai prdqrrsest feifcpgsrt fqfvarnrkn
      241 meewiakice lggaekpdhk tsstkaaina svesdttrpd eqknsnckee ryqdvgslti
      301 dnvseeasil tnvvvsehid kettndsssk dtsrettnss sssslppllv stctivpaas
      361 silspppppp ppppppsspa rilntssfsq iprklpslpv hlspyeflde eeddiyhkie
      421 dfrdstrygn vgetetdnar skkepvtydd vranikgest sdheqtrkds knvarkslnl
      481 qnettyddtt ttntvskdtk kvavneqakf vsyddvenla larstkvrgt dktdestksp
      541 qkksfldrvr skkespkkne kkvkckpapa svstsllvnd qeplpiyydd vsdltnvqqm
      601 krfeeensey tcppaprpiy vkpptivgtv tdsqefyddv gtyrerhekd hdrdfckfhq
      661 pkkhnygrdq vptdndrsll ceniqdnehy ktpridaghp ksvlidqrde lyddiailad
      721 ftarqkeyfg kknseeitkl qvspekrswn rfvsgkkskt fdstvsetnn srvsngtedt
      781 tddfneqqss mrmntiqkli srmensfgka svrtassmll nktnvanna
//
LOCUS       XP_034172744             383 aa            linear   INV 03-SEP-2025
DEFINITION  pyruvate dehydrogenase E1 component subunit alpha,
            mitochondrial-like isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034172744
VERSION     XP_034172744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="pyruvate dehydrogenase E1 component subunit
                     alpha, mitochondrial-like isoform X2"
                     /calculated_mol_wt=43131
     Region          34..383
                     /region_name="TPP_enzymes"
                     /note="Thiamine pyrophosphate (TPP) enzyme family,
                     TPP-binding module; found in many key metabolic enzymes
                     which use TPP (also known as thiamine diphosphate) as a
                     cofactor. These enzymes include, among others, the E1
                     components of the pyruvate, the acetoin and...; cl01629"
                     /db_xref="CDD:470272"
     Site            order(87,139,164..165,196,198..201,204,208,212,227..230,
                     244,294,298)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(117..118,164,166,194..197,224,226,290)
                     /site_type="other"
                     /note="TPP-binding site [chemical binding]"
                     /db_xref="CDD:238958"
     Site            order(159,161,163,168,171..172,175..176,178..179,182,
                     201..202,208..209,212)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(285..291,293..303,312..314)
                     /site_type="phosphorylation"
                     /note="phosphorylation loop region [posttranslational
                     modification]"
                     /db_xref="CDD:238958"
     CDS             1..383
                     /gene="LOC117600857"
                     /coded_by="XM_034316853.2:579..1730"
                     /db_xref="GeneID:117600857"
ORIGIN      
        1 myfywsrllr scrnqrylff trrremsndn dktitvdtky dlyrlengps ekatinekea
       61 myalktmiyi rrmenkaael yklrlingfl hlysgqeava vgmkmsmkeq dslitayrch
      121 gfavvfgasa revfaelmgr ktgiskgkgg smhmyakqfy ggdgivggqv pigaglgfae
      181 kyngtggvaw tlygdgaasq gqvhetynis qlwnlpvvyi cennqygmgt aihrhsantk
      241 fytrgdlipg vkvdgmkvad vceaikfard havrrgpiil emvtyryfgh smsdpgtsyr
      301 treevksvrd erdpiklfiq vlvenglktt aeiqqiqasv nkvvdeeleq akadpppems
      361 eiganvyvkp lekirgkvpw evh
//
LOCUS       XP_034172754             383 aa            linear   INV 03-SEP-2025
DEFINITION  pyruvate dehydrogenase E1 component subunit alpha,
            mitochondrial-like isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034172754
VERSION     XP_034172754.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316863.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="pyruvate dehydrogenase E1 component subunit
                     alpha, mitochondrial-like isoform X2"
                     /calculated_mol_wt=43131
     Region          34..383
                     /region_name="TPP_enzymes"
                     /note="Thiamine pyrophosphate (TPP) enzyme family,
                     TPP-binding module; found in many key metabolic enzymes
                     which use TPP (also known as thiamine diphosphate) as a
                     cofactor. These enzymes include, among others, the E1
                     components of the pyruvate, the acetoin and...; cl01629"
                     /db_xref="CDD:470272"
     Site            order(87,139,164..165,196,198..201,204,208,212,227..230,
                     244,294,298)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(117..118,164,166,194..197,224,226,290)
                     /site_type="other"
                     /note="TPP-binding site [chemical binding]"
                     /db_xref="CDD:238958"
     Site            order(159,161,163,168,171..172,175..176,178..179,182,
                     201..202,208..209,212)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(285..291,293..303,312..314)
                     /site_type="phosphorylation"
                     /note="phosphorylation loop region [posttranslational
                     modification]"
                     /db_xref="CDD:238958"
     CDS             1..383
                     /gene="LOC117600857"
                     /coded_by="XM_034316863.2:531..1682"
                     /db_xref="GeneID:117600857"
ORIGIN      
        1 myfywsrllr scrnqrylff trrremsndn dktitvdtky dlyrlengps ekatinekea
       61 myalktmiyi rrmenkaael yklrlingfl hlysgqeava vgmkmsmkeq dslitayrch
      121 gfavvfgasa revfaelmgr ktgiskgkgg smhmyakqfy ggdgivggqv pigaglgfae
      181 kyngtggvaw tlygdgaasq gqvhetynis qlwnlpvvyi cennqygmgt aihrhsantk
      241 fytrgdlipg vkvdgmkvad vceaikfard havrrgpiil emvtyryfgh smsdpgtsyr
      301 treevksvrd erdpiklfiq vlvenglktt aeiqqiqasv nkvvdeeleq akadpppems
      361 eiganvyvkp lekirgkvpw evh
//
LOCUS       XP_034172765             334 aa            linear   INV 03-SEP-2025
DEFINITION  pyruvate dehydrogenase E1 component subunit alpha, somatic form,
            mitochondrial-like isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034172765
VERSION     XP_034172765.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316874.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..334
                     /product="pyruvate dehydrogenase E1 component subunit
                     alpha, somatic form, mitochondrial-like isoform X3"
                     /calculated_mol_wt=37651
     Region          34..334
                     /region_name="TPP_enzymes"
                     /note="Thiamine pyrophosphate (TPP) enzyme family,
                     TPP-binding module; found in many key metabolic enzymes
                     which use TPP (also known as thiamine diphosphate) as a
                     cofactor. These enzymes include, among others, the E1
                     components of the pyruvate, the acetoin and...; cl01629"
                     /db_xref="CDD:470272"
     Site            order(87,139,164..165,196,198..201,204,208,212,227..230,
                     244,294,298)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(117..118,164,166,194..197,224,226,290)
                     /site_type="other"
                     /note="TPP-binding site [chemical binding]"
                     /db_xref="CDD:238958"
     Site            order(159,161,163,168,171..172,175..176,178..179,182,
                     201..202,208..209,212)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(285..291,293..303,312..314)
                     /site_type="phosphorylation"
                     /note="phosphorylation loop region [posttranslational
                     modification]"
                     /db_xref="CDD:238958"
     CDS             1..334
                     /gene="LOC117600857"
                     /coded_by="XM_034316874.2:579..1583"
                     /db_xref="GeneID:117600857"
ORIGIN      
        1 myfywsrllr scrnqrylff trrremsndn dktitvdtky dlyrlengps ekatinekea
       61 myalktmiyi rrmenkaael yklrlingfl hlysgqeava vgmkmsmkeq dslitayrch
      121 gfavvfgasa revfaelmgr ktgiskgkgg smhmyakqfy ggdgivggqv pigaglgfae
      181 kyngtggvaw tlygdgaasq gqvhetynis qlwnlpvvyi cennqygmgt aihrhsantk
      241 fytrgdlipg vkvdgmkvad vceaikfard havrrgpiil emvtyryfgh smsdpgtsyr
      301 treevksvrd erdpiklfiq vlvenglktt aeiq
//
LOCUS       XP_076546474             736 aa            linear   INV 03-SEP-2025
DEFINITION  putative methyltransferase NSUN7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546474
VERSION     XP_076546474.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690359.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="putative methyltransferase NSUN7 isoform X1"
                     /calculated_mol_wt=84086
     CDS             1..736
                     /gene="LOC117600852"
                     /coded_by="XM_076690359.1:1..2211"
                     /db_xref="GeneID:117600852"
ORIGIN      
        1 mekvsemeeg vgrggwewep kfrgfislka tvrrsgreei ngslknvrgv ggsmteeyvq
       61 pgwrindill aarilrkvqp spiffdeaem rrvfglvydv lrykkifdra lkdigfwrrn
      121 namrdreriv wlllydmqgr kfakqremda vkerktifea agladienal leakthlaas
      181 isrqrirgsa lsldellpsr lrviegvawg keaaiafgwv niakldnkee fleemsklgl
      241 vcrqdddttm eleetsyafd sicpklinfh ekareklavs slvrdhrfif lerslcigaa
      301 tlakairigr fcgpvilthc laprhtayla qllteidhag kllafgtgdr rceyetylrk
      361 lgvtlqqcrv fsekyvshsa taelerasvv lavppssytg vkdivdlava rggdsdlles
      421 ltnaytsddn rndhrypqaf ladqmstlky altrpnvqfl iyevhtilss ettvmvqrvv
      481 dyvnrlatek yvrehpkkvs keakellkfe kmdgsreedr vstisanvtv pdsdlfevgs
      541 iddiygknas clldsgcfla vikrkemmqf dslfminvae skglfgdpke rqeakqqslp
      601 prslesnvrk rakrikieid rimahtyssl srsksgtric prhlrynsdw ketirstmqv
      661 senveenssk swqhdkvnan rlntlactep qqekassftd yrffnrpigd ftwrlflrdy
      721 dktgdtsdql vvlder
//
LOCUS       XP_076546475             671 aa            linear   INV 03-SEP-2025
DEFINITION  putative methyltransferase NSUN7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546475
VERSION     XP_076546475.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690360.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..671
                     /product="putative methyltransferase NSUN7 isoform X2"
                     /calculated_mol_wt=76464
     CDS             1..671
                     /gene="LOC117600852"
                     /coded_by="XM_076690360.1:1..2016"
                     /db_xref="GeneID:117600852"
ORIGIN      
        1 mekvsemeeg vgrggwewep kfrgfislka tvrrsgreei ngslknvrgv ggsmteeyvq
       61 pgwrindill aarilrkvqp spiffdeaem rrvfglvydv lrykkifdra lkdigfwrrn
      121 namrdreriv wlllydmqgr kfakqremda vkerktifea agladienal leakthlaas
      181 isrqrirgsa lsldellpsr lrviegvawg keaaiafgwv niakldnkee fleemsklgl
      241 vcrqdddttm eleetsyafd sicpklinfh ekareklavs slvrdhrfif lerslcigaa
      301 tlakairigr fcgpvilthc laprhtayla qllteidhag kllafgtgdr rceyetylrk
      361 lgvtlqqcrv fsekyvshsa taelerasvv lavppssytg vkdivdlava rggdsdlles
      421 ltnaytsddn rndhrypqaf ladqmstlky altrpnvqfl iyevhtilss ettvmvqrvv
      481 dyvnrlatek yvrehpkkvs keakellkfe kmdgsreedr vstisanvtv pdsdlfevgs
      541 iddiygknas clldsgcfla vikrkemmqf dslfminvae skglfgdpke rqeakqqslp
      601 prslesnvrk rakrikieid rimahtyssl srsksgtric prhlrynsdw ketirstmqd
      661 mlracyihvs m
//
LOCUS       XP_076546476             671 aa            linear   INV 03-SEP-2025
DEFINITION  putative methyltransferase NSUN7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546476
VERSION     XP_076546476.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690361.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..671
                     /product="putative methyltransferase NSUN7 isoform X2"
                     /calculated_mol_wt=76464
     CDS             1..671
                     /gene="LOC117600852"
                     /coded_by="XM_076690361.1:1..2016"
                     /db_xref="GeneID:117600852"
ORIGIN      
        1 mekvsemeeg vgrggwewep kfrgfislka tvrrsgreei ngslknvrgv ggsmteeyvq
       61 pgwrindill aarilrkvqp spiffdeaem rrvfglvydv lrykkifdra lkdigfwrrn
      121 namrdreriv wlllydmqgr kfakqremda vkerktifea agladienal leakthlaas
      181 isrqrirgsa lsldellpsr lrviegvawg keaaiafgwv niakldnkee fleemsklgl
      241 vcrqdddttm eleetsyafd sicpklinfh ekareklavs slvrdhrfif lerslcigaa
      301 tlakairigr fcgpvilthc laprhtayla qllteidhag kllafgtgdr rceyetylrk
      361 lgvtlqqcrv fsekyvshsa taelerasvv lavppssytg vkdivdlava rggdsdlles
      421 ltnaytsddn rndhrypqaf ladqmstlky altrpnvqfl iyevhtilss ettvmvqrvv
      481 dyvnrlatek yvrehpkkvs keakellkfe kmdgsreedr vstisanvtv pdsdlfevgs
      541 iddiygknas clldsgcfla vikrkemmqf dslfminvae skglfgdpke rqeakqqslp
      601 prslesnvrk rakrikieid rimahtyssl srsksgtric prhlrynsdw ketirstmqd
      661 mlracyihvs m
//
LOCUS       XP_034172798             613 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear localized protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034172798
VERSION     XP_034172798.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316907.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172798.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..613
                     /product="nuclear localized protein 1"
                     /calculated_mol_wt=70514
     Region          227..556
                     /region_name="Tcf25"
                     /note="Transcriptional repressor TCF25; pfam04910"
                     /db_xref="CDD:461476"
     CDS             1..613
                     /gene="Nulp1"
                     /coded_by="XM_034316907.2:244..2085"
                     /db_xref="GeneID:117600885"
ORIGIN      
        1 mstrymkkvy gsdvllekdn eneneiensi ignvksktfn lfdvlnense vsekeneeep
       61 viedncdnet krkrrkkrrk kfensktqqa igtkqeeeee eeeeeegeed ideiertvre
      121 vnkllgeplp gcssqstdnl qwieqksked illvqhkhln pynelkrifg sktvqaeqnn
      181 rrnrgrlghl kktwlvstrn nwppvnksgl smsldhtvks annvqyfvye hspsyrqvql
      241 kfleavesln penivsilna hsyhvdallq iaelcklned lamaaefter alyclecafh
      301 plfnvttalc rldyrkqqnr alfitlfkhl nfvggracyr tslefcklll sldpegdpla
      361 vilsldffal rakeyewfie fcnlwdnarn ltqlpniafs lalahfhlgh qtvasellqn
      421 alimfsgvlm plldkcsiqc detvrihdff nskavistsp aleklqnlyv arsfrlwket
      481 dllpwlrenv hivltrvdsk ddyvkycevk rskryqgklp knilrhiils dikdvtvniq
      541 eiqnggsvls hdplppvdsi diykrptant tttrtssnll slfvsslftd ingevsvaal
      601 eglnlfydnn eqa
//
LOCUS       XP_034172908             477 aa            linear   INV 03-SEP-2025
DEFINITION  pyruvate dehydrogenase [acetyl-transferring]-phosphatase 1-like
            protein, mitochondrial [Osmia lignaria lignaria].
ACCESSION   XP_034172908
VERSION     XP_034172908.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317017.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="pyruvate dehydrogenase
                     [acetyl-transferring]-phosphatase 1-like protein,
                     mitochondrial"
                     /calculated_mol_wt=53410
     Region          64..383
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(75,79..80,99..101,361)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..477
                     /gene="Pdp"
                     /coded_by="XM_034317017.2:202..1635"
                     /db_xref="GeneID:117600930"
ORIGIN      
        1 mvlqnissnl iktvantyik qnkrnsntci qrsymtiprl tpqevtavlq tneytkefng
       61 dssikyydsn qlgsnnpied trseaqcllt kgillgvfdg hggstcaqiv skrlfhyisa
      121 cllptkllkq ylntvnsnnk lellqtfndk vefvseikdl yqasflnfvk dligtectke
      181 fqmetalena flrldndlsn eallkldknn aartlavams gavaavahid gphlhvagvg
      241 dcqavlgifs endgwsaklm tvehntdnra everilsehp lnekstvikm erllgqlvpl
      301 rslgdfrykw skkiikevvv pyfgeaaipp nyhtppylta kpdvkyhrlt prdkfliias
      361 dglwdlispl qavrlvgehm sgkvtlsplr lprknmklsd inkmllqrke glkkkpldsn
      421 aathllrnal ggteygidha klsrlltlps evgrifrddi titvvymdse flrhcpp
//
LOCUS       XP_034172943             440 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 4 [Osmia lignaria lignaria].
ACCESSION   XP_034172943
VERSION     XP_034172943.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317052.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..440
                     /product="vacuolar protein sorting 4"
                     /calculated_mol_wt=49110
     Region          7..75
                     /region_name="MIT_VPS4"
                     /note="MIT: domain contained within Microtubule
                     Interacting and Trafficking molecules. This sub-family of
                     MIT domains is found in intracellular protein transport
                     proteins of the AAA-ATPase family. The molecular function
                     of the MIT domain is unclear; cd02678"
                     /db_xref="CDD:239141"
     Region          124..294
                     /region_name="RecA-like_VPS4"
                     /note="ATPase domain of vacuolar protein
                     sorting-associated protein 4; cd19521"
                     /db_xref="CDD:410929"
     Site            order(125,144,148,151..152,156,160,172..173,177,181,192,
                     194,196..198,201,209,230..231,234,239,241,243..245,
                     247..249,252..253,276,279)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:410929"
     Site            order(131..133,135,172..178,230,275)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:410929"
     Region          319..359
                     /region_name="AAA_lid_3"
                     /note="AAA+ lid domain; pfam17862"
                     /db_xref="CDD:465537"
     Region          377..437
                     /region_name="Vps4_C"
                     /note="Vps4 C terminal oligomerization domain; pfam09336"
                     /db_xref="CDD:462762"
     CDS             1..440
                     /gene="Vps4"
                     /coded_by="XM_034317052.2:331..1653"
                     /db_xref="GeneID:117600941"
ORIGIN      
        1 malgtilqka idlvtkatee drnknyeeal rlyehaveyf lhsikyeaqg drakesirak
       61 ctqyleraen lkaylkkkkk pvkagednsk aedkksdsgd sdtdsdpekk klqtklegai
      121 iiekpdikws dvagldgake alkeavilpi rfphlftgkr ipwkgillfg ppgtgksyla
      181 kavateanns tffsvsssdl vskwlgesek lvknlfdlar qhkpsiifid eidslcssrs
      241 dnesesarri kteflvqmqg vgsdndgilv lgatnipwvl dsairrrfek riyiplpdeq
      301 araimfklhl gntahyltee dfkklaaatd gysgadisii vrdalmqpvr qvqtathfkr
      361 vrgpspkdps iivddlltpc spgdpaaiem swmevegdkl yeppvtmkdm lkslattrpt
      421 vneedmtkle kfkedfgqeg
//
LOCUS       XP_034172969             417 aa            linear   INV 03-SEP-2025
DEFINITION  actin-related protein 3 [Osmia lignaria lignaria].
ACCESSION   XP_034172969
VERSION     XP_034172969.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317078.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..417
                     /product="actin-related protein 3"
                     /calculated_mol_wt=46762
     Region          7..409
                     /region_name="ASKHA_NBD_Arp3-like"
                     /note="nucleotide-binding domain (NBD) of actin-related
                     protein3 (Arp3) and similar proteins; cd10221"
                     /db_xref="CDD:466822"
     CDS             1..417
                     /gene="Arp3"
                     /coded_by="XM_034317078.2:142..1395"
                     /db_xref="GeneID:117600956"
ORIGIN      
        1 mlgrlpacvi dvgtgytklg fafnkepqlm ipsaiaiket akvgdnnarr vtkgvedlda
       61 yigdeafeat gysvkypvrh glvedwdlme kflqqcifky lraepedhyf lltepplntp
      121 enreytaeim fesfnvpgly iavqavlala aswtaksven rtltgvvvds gdgvthvipv
      181 aegfvigsci khipiagrdi tyfiqsllre reigippeqs letakaikek ycyicpdisk
      241 efakydsdpt kikkyegins itkqpfvvdv gyerflgpei ffhpefsnpd fttplseivd
      301 dviqncpidv rrplysnivl sggstmfkdf grrlqrdikr ivdarlklse tlsgshitpk
      361 pidvhvishh kqrcavwygg allasdpefy tvchtkkayq eygpgicryn pvfhsmv
//
LOCUS       XP_034172983             376 aa            linear   INV 03-SEP-2025
DEFINITION  beta-parvin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034172983
VERSION     XP_034172983.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317092.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="beta-parvin isoform X1"
                     /calculated_mol_wt=42868
     Region          101..205
                     /region_name="CH_PARVA_B_rpt1"
                     /note="first calponin homology (CH) domain found in the
                     alpha/beta parvin subfamily; cd21304"
                     /db_xref="CDD:409153"
     Site            order(102,106,157,159..160,163..164,166,177..185,190,
                     192..193,195..196,199..200,203)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409153"
     Region          252..372
                     /region_name="CH_PARVA_B_rpt2"
                     /note="second calponin homology (CH) domain found in the
                     alpha/beta parvin subfamily; cd21306"
                     /db_xref="CDD:409155"
     Site            order(268,272,326,328..329,332..333,335,344..352,357,
                     359..360,362..363,366..367,370)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409155"
     CDS             1..376
                     /gene="parvin"
                     /coded_by="XM_034317092.2:68..1198"
                     /db_xref="GeneID:117600961"
ORIGIN      
        1 mkkkrvfgik lahwgeknvl kkvsydsern iivnlgcasf kkfiesisly vqevqeegky
       61 aidspgyaan pemppeeyal deneersmie prsledsklk elifvliewi ndeladqrii
      121 vkdiaedlyd gqvlqkllek ltgekldvpe vtqseegqkq klavvlstan rvlchyppyk
      181 wsvelvhskn ivailhllvg larqfrapvr lpervaaqvv vvrkkdgqli hktvreeits
      241 tyddlgmrce rdafdslfdd gpdklavvkr slitfvnkhl skvhlevtdl dtqfhdgvfl
      301 tlllgllegf fvplgsfhlt pkthdqkvhn vsfafdimqd iglpkpkarp edivnldlks
      361 tlrvlynlft kyksmn
//
LOCUS       XP_034172995             366 aa            linear   INV 03-SEP-2025
DEFINITION  beta-parvin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034172995
VERSION     XP_034172995.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317104.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="beta-parvin isoform X2"
                     /calculated_mol_wt=41734
     Region          91..195
                     /region_name="CH_PARVA_B_rpt1"
                     /note="first calponin homology (CH) domain found in the
                     alpha/beta parvin subfamily; cd21304"
                     /db_xref="CDD:409153"
     Site            order(92,96,147,149..150,153..154,156,167..175,180,
                     182..183,185..186,189..190,193)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409153"
     Region          242..362
                     /region_name="CH_PARVA_B_rpt2"
                     /note="second calponin homology (CH) domain found in the
                     alpha/beta parvin subfamily; cd21306"
                     /db_xref="CDD:409155"
     Site            order(258,262,316,318..319,322..323,325,334..342,347,
                     349..350,352..353,356..357,360)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409155"
     CDS             1..366
                     /gene="parvin"
                     /coded_by="XM_034317104.2:13..1113"
                     /db_xref="GeneID:117600961"
ORIGIN      
        1 mmasprpksp rppistkkde keesfwdkig tlgrkkrike vqevqeegky aidspgyaan
       61 pemppeeyal deneersmie prsledsklk elifvliewi ndeladqrii vkdiaedlyd
      121 gqvlqkllek ltgekldvpe vtqseegqkq klavvlstan rvlchyppyk wsvelvhskn
      181 ivailhllvg larqfrapvr lpervaaqvv vvrkkdgqli hktvreeits tyddlgmrce
      241 rdafdslfdd gpdklavvkr slitfvnkhl skvhlevtdl dtqfhdgvfl tlllgllegf
      301 fvplgsfhlt pkthdqkvhn vsfafdimqd iglpkpkarp edivnldlks tlrvlynlft
      361 kyksmn
//
LOCUS       XP_034173022             329 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein S35 [Osmia lignaria lignaria].
ACCESSION   XP_034173022
VERSION     XP_034173022.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173022.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="mitochondrial ribosomal protein S35"
                     /calculated_mol_wt=38237
     Region          <11..>71
                     /region_name="beta-trefoil_IL1"
                     /note="beta-trefoil domain found in the interleukin-1
                     (IL-1) family of cytokines; cl00094"
                     /db_xref="CDD:469612"
     Region          177..>241
                     /region_name="MRP-S28"
                     /note="Mitochondrial ribosomal subunit protein; pfam10213"
                     /db_xref="CDD:463002"
     CDS             1..329
                     /gene="mRpS35"
                     /coded_by="XM_034317131.2:135..1124"
                     /db_xref="GeneID:117600980"
ORIGIN      
        1 misltkcyea nkiivalkir klslpfstas stnddtefrv lqlipeekkt gpkrkhsqlf
       61 vinrsttkak tmptnqnwqa iwpaartfhp dvvplplrqg yknskgiypd kyanaelmki
      121 pnflhltppv ikkhcealkk fctkwpkele tseksvqyfp ieistsdycy ssptirepla
      181 rivtlrvkls slhldghakd kmlrlvgdry nsktdqitiv adrcptrkqn ldyakyllta
      241 lyhiswries weaekseddm eyyywdkrks rqtltavhcw pnsptdtnye siphateykn
      301 avselmnnee nhfflnqykq avknvlnik
//
LOCUS       XP_034173053             321 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat-containing protein 59 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173053
VERSION     XP_034173053.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317162.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="leucine-rich repeat-containing protein 59"
                     /calculated_mol_wt=36791
     Region          <13..>116
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          13..35
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          36..58
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          59..81
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          82..104
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          105..128
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..321
                     /gene="LOC117600988"
                     /coded_by="XM_034317162.2:363..1328"
                     /db_xref="GeneID:117600988"
ORIGIN      
        1 mnlksvkdrl kdetldlslc dlkevpvrei ttikkathld lsnnlltslp tnfinlkqiv
       61 kldlsrnmlt eipenfgelr qlkhldlyan qisrlplsls elknlrwldl kenpltpava
      121 svagpcsnls ecqacarniv tylshvklti eeeklrrlna iidtetdtvs tkktgkkkkk
      181 kaadkdnkpn ldknernslp klsrsdesem knstatndrk ytdkqsikgn tcrtflhtit
      241 wlflfssill mlililplys kqsellvnhf etnmgiplkt iqqrstdmfy sftrsvsnvy
      301 kdiyyvyekn fntnkdisin e
//
LOCUS       XP_076546477            4640 aa            linear   INV 03-SEP-2025
DEFINITION  MYC binding protein highwire [Osmia lignaria lignaria].
ACCESSION   XP_076546477
VERSION     XP_076546477.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690362.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4640
                     /product="MYC binding protein highwire"
                     /calculated_mol_wt=510250
     Region          <468..662
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          848..>963
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          1174..1324
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     Region          1670..1827
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     Region          <2331..2373
                     /region_name="IG_FLMN"
                     /note="Filamin-type immunoglobulin domains; smart00557"
                     /db_xref="CDD:214720"
     Region          2404..2459
                     /region_name="SH3_3"
                     /note="Bacterial SH3 domain; pfam08239"
                     /db_xref="CDD:462405"
     Region          2526..>2565
                     /region_name="SH3_3"
                     /note="Bacterial SH3 domain; pfam08239"
                     /db_xref="CDD:462405"
     Region          <3730..3848
                     /region_name="APC10-like"
                     /note="APC10-like DOC1 domains in E3 ubiquitin ligases
                     that mediate substrate ubiquitination; cl02148"
                     /db_xref="CDD:382862"
     Site            order(3739,3769,3776,3778,3834)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:176484"
     Region          4222..4270
                     /region_name="Bbox2_MYCBP2"
                     /note="B-box-type 2 zinc finger found in Myc-binding
                     protein 2 (MYCBP2) and similar proteins; cd19799"
                     /db_xref="CDD:380857"
     Region          4388..4442
                     /region_name="RING-H2_PHR"
                     /note="RING finger, H2 subclass, found in the PHR
                     (Pam/Highwire/RPM-1) protein family; cd16463"
                     /db_xref="CDD:438126"
     CDS             1..4640
                     /gene="hiw"
                     /coded_by="XM_076690362.1:47..13969"
                     /db_xref="GeneID:117608635"
ORIGIN      
        1 mvsgdhekll pepenyvkcf ydlfksiaea qrnkeewrkc kknksirkrd kkkidltgdl
       61 nynsppeiel pcnasafavf asvrsailek harttnevyr vsncnspspp ehsetmdtes
      121 ddedristiq slpkivgigl rsvftlmres raiepllctk alsalldilq gqlpeglkse
      181 pddvidplfd llldlatshg pesaaandgs hltavacacl lslvvvrgdt grllaaiaal
      241 lmcpralavq niqmpcvlts lqrsvqavll gklirpdwit ygvpkcskiy tstlklpnei
      301 nnitlngrsf vsdgkylylh tnrgllkigs ghggtiwghm yiqkadfypt etgwlgyann
      361 tlyfkctprk qselliidae tlvvtgiavl egrdwsssvm fsdgeclgmi tagkddgfvv
      421 rtintlsnpv avaselplkl arkcvdvfgy safdeeqavh ilnpgcddei atvtagkefg
      481 llktvsgkvl ytgkgtclgm ksntrpnrwv eltlgkgprl vhfaaghdgq hvvmimedgs
      541 vlfagtarrg edgdnnkvrr qpkpvkpkki akvegqfivd aacnngstal vtkegallmf
      601 gkdtlhsdpv sgvvtdlrdv cvvhvslgka haatltnkgh lytfginnkg qcgrdftavh
      661 avnkdvsvva memgtaddel lvteedgvep tedweetrgm cppglhqwkh rvcmvctvcr
      721 ectgcsiscl ssirpdrnpg qecgcgkgds gcaecgccrt carkscttgr sssnfreylq
      781 rrlgeikqkq rtkagpstak ygmkmkgpnn qklagpsviq kstgkmalgl stnlmieegi
      841 ggsdiergda ariasippar vpipsespvv qvscglhhtv vllqngevyt fgsniygqlg
      901 vgdlvahagv vhvklpgiat qvaagsnhtv iltskgevft fgayqkgqlg mnwwngqnds
      961 snttsqtsrr sdrtqpwhsf pnlvpnigpr wgrratwvga sgdqtyvkid einfisltrs
     1021 tvmankncii liphqtdhan sfkclviskr dgtcnsysga dqvdfsncat cldplynviw
     1081 tfnpikseis lyniistear tipglevsil npglalpvvs scfvtrsqaa mhllacldtl
     1141 tqaqdenlti veenecnqst hgkaysredf vtvnrfeshg ggwgytahsi eairfmpdtd
     1201 illggyglfg grgeytakik lfdigtdggd qendgellae seevpyecgp rqkysilfde
     1261 pialqanrwy vawakvsgps sdcgtggqgm vtakdqvmfy fksskrsnng tdvnagqipq
     1321 llyrivtpes qtpnrqrdqv epvyvlkrdf srtvtkecfq slisllqwsw ntlkaalndt
     1381 mvhaisshal lemerlvyis kaslrllrty ineiypnqvg kktpaesvrl aecigevral
     1441 lhqilsdsvp lntkskgktr snkstttisn kltnsildec hrtfvacyha fyptaylkwt
     1501 clcellseid reqgrsskdr llsavlaslc npairlrctf piltnimdss dsikrqlsps
     1561 dntgllmins teshqypilv eqisyksqve ssgketlnws frdvldrlld lilvpvkksl
     1621 ckektqslpe lvlhccylla rviaelaaqs sgnedelqaa cgrlmyttps rftrvnqsrs
     1681 wntgngspda icfsvdrpgi vivgvgiygg vgmydyelel lddqnntgnd pshtqrwssl
     1741 dftrgsfgpd dcvndivelk fdkpvliken vkyairlrnr ggrtsngdgg lsvvkgpdgt
     1801 tftftacsls fngttqtrgq iphilyysnp qdsdgqhtsk amaevqarkc tlamtatviq
     1861 rsneilalar eraedgiate vlgnatfitt llplvmahis platsdprsg vqvltliqem
     1921 lphvsalnll samglsqisq dseaqshvsp pittshhytw lesdhpykpa tvshykvtfp
     1981 dtvkwltief tpecgtaqpe dylqlyipni ispvgtsvta gvedtplywp vlhklsnvqs
     2041 qwpqnaivlp gnevifslet asdymkndra vmygfkclvi gydwitsgng lknleielsf
     2101 lggacaaslm krnlalppvs neeveedlel mqetakrifs mhstllgrgf alaspptvsq
     2161 aldgvlpfsc hsnerlflrd fvscsagtsg grlarwlqpd sfvdpskcea lyskeemkcg
     2221 wpaivtiltr dqygdvvhvp nlkvevkavp idkkdlaesd qgrkirrvsq pdpmsfgghp
     2281 qppldvpyev tinnktcfya itimkayqny sfeelrfmsp tikrssesml vrpngdgsys
     2341 atwtpssvgw yslmitvdgy nmednyrvev keppqgmqpp trnivkkpqh qpsrlrkfvv
     2401 knsaglrira hpslqseqig ivsvngtiaf ideihnddgv wlrlnaetik kfcssshlea
     2461 wclqynqhlg ktlllpveep ksildqvikd tilrkrpevv ddkrktvtfd ggrsmvvvkc
     2521 gasghnirsr pslkaapvgm lalgnsvtiq eyfannegtw vriddesmak ycfdkgrvae
     2581 geawslaink hgvtymkteq dlendpvekk pivadpsvsp nhkgfdfsvp svsnegfnft
     2641 aqnytpgtae sgegsntnpf ifgsynqhds pgsdkftpek tdvspkftkp hskermvker
     2701 ereggggggk fsvlqkwlrg ddkchgekks spgsfgicrd fsefvgvsvk elvkamgesr
     2761 angngatppe tprrlsrsss pknpqggspr fhspssspvp ipagssrqpv gsgnclfppa
     2821 ahapvsggmv dsitsqrrgs tqsdtsalvs sltrdpsqsp savsttantr dlspspscss
     2881 lhmrsegsia sppdtpkqdg gdsqesprkv sqaqtqtspe sattamkghf sigsagpkee
     2941 rhpaklsrre hskiaktrtk raispttsqs pspnrtlnlt kdkvkeaisp svaeclravf
     3001 aaflwhegiv hdamacasfl kfhpslpkqg alvvtrqtvl qstdkqkqeq karqrhsvev
     3061 tnagnylhiq pstletltrs aanananrnr kkqettikee vqengklgal pefqtvavlp
     3121 palkslvflw eelstnclqt ieqrsvlpsp isqiqttkls krpekrired kvkerekkts
     3181 kkkkewkpvg rangsealgg iereticelc glmfphpvty hmkmmhpgcg whaggkgyns
     3241 ggnycigwag ncgdggvggs swylicdtcr dkylrarklk lakkfltgmt kkkngtgkiv
     3301 splgspngne thiimknnam flldlasasg lnipkqqrrp sqtlssvaen ysppeaagpf
     3361 pptgpfqclq algvhnsqsh deryyeetlr rqnghpngye gnngmsnttn grplsehpis
     3421 dsdnestkgr gnfhrsvsms taapwtrntn dgrvvmmrkr nnsssemnne agssllcyps
     3481 aalqklvpsm nqsaivstsq aevtntdkie ilvrpvmlfv lqqhnlqhlq lamkqalrra
     3541 acrvyamqal nwllrsvtqp iclhdllwwf vaslspivsp diadandedn rtdrkedhdm
     3601 igvcehplsd lviagesvnp lptvfhtllq tiadlmllpp pgsplqqaai rcwgikftpa
     3661 dhmflhrshv fsniskilsr seeeedttls mheshqsvhs qqvnscvevl kdltcgveik
     3721 assrqamivs ltdnstetfw esgdedrnkt ktisivcgah siprvvyihi dncrdlthkv
     3781 ssvtfqsgsn ademvklrtv eiesrsagwi hcpvtdprhi ivrldlkgpd nslrvrqiri
     3841 lgeiegeslk igkqlsaqti qqrnceaetl kvfrlitsqv fgkliqgeqq qqqteaaega
     3901 nedledsndl rehmvgilfs rnnlthlqkq vcthivqaiq ketirmreew eallcspapa
     3961 nsllsdnsdl pkaadtycfe mlsmvlalsg ssvgqyylsh qygllkdlls llhtgsarvq
     4021 rqvtsllkri lpeikpetla nvisikrlpp tdfsivsaan ngfthtsefd ehspgildvf
     4081 lsciakaltv qvkvkgkenn gkalqtasla tsihpksyvg prwwlrgcmt rklaeviiql
     4141 lkdmasgkls eawasvtkaa iaenilnltr ldeksrdpse clktptlwla lsslcvldsd
     4201 hverlssgqw tgsegqpppp rptcsnhddg ettaiiqcnv cgnlcaecdr ilhlhrrtrm
     4261 hirqvckeee eairvdlheg cgrtklfwil aladsrtlka lvefrdgspr kpvgatsgic
     4321 rfcgttentg llaignvcad hdcqehakna ctkvhscghi cggvrneknc lsclhrclpg
     4381 sdlkqdaddm cmicfteals aapaiqlqcg hvfhlhccrh vlmkrwvgpr itfgfslcpi
     4441 ckvpmehstl adqlasikel yedvrrkalm rleyeglnka eavfgpggry hqdpaayame
     4501 ryayyvcykc qkayyggear cdaqiggetf dpaelvcggc sdvarahmcp khgadfleyk
     4561 cryccsvavf fcfgtthfcn pchddfqrvt tipkselpsc pagpkakqle gdecplhvkh
     4621 pptgeefalg cgicrnahtf
//
LOCUS       XP_076546478             989 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 47 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546478
VERSION     XP_076546478.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690363.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..989
                     /product="WD repeat-containing protein 47 isoform X1"
                     /calculated_mol_wt=109196
     Region          74..123
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     Region          <668..987
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          671..719
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          725..766
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          771..812
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          820..858
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          872..908
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          914..953
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          960..984
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..989
                     /gene="LOC117608649"
                     /coded_by="XM_076690363.1:598..3567"
                     /db_xref="GeneID:117608649"
ORIGIN      
        1 mvvvassait viedfskdtl grlriryvmp aahltlreed vvrltleflh srdlhisqls
       61 leretgving qysddvlflr qlildgqwdd vlefiqplea lpdfdmrkft ysilrhkyve
      121 llcikseanl natgtngsvd naveevvkvl sdlekvapsk eeysslclll tlprltdhlq
      181 ykdwnpsnar vqcfrevhll vekflpgdrk stdpahpvss akndrliqli ikgilyescv
      241 nycqakatgs keseqvemnf srlldgsvgf sdsdlsllsw lqsippetfa vpfaqrtlnv
      301 dverlerpsl etswtehmli tpikpktfph campftrprs aadmmsrslv paleaglgpr
      361 spgpkntpip saalmalstg dinpmsrssf asfhltgfkn nklmntsvdr lfenegdvfl
      421 sssyaeyqql psiqetttns qpkapprtrg qskspegika dpsaastper riagrespap
      481 starssrrds laekptgvaa kitepsvipg rpsiggdhle qsyngdlfke yqkqkqrlqe
      541 kfqqrerewd dlvrqlsapl spqvvdnrlq ndgikqplgs kgpspvhtst parsgiqspk
      601 pvingsepmv rqnsalgnaf dprvqeshyd vvkpikqepk qeldasngss nggrprfvpv
      661 taledvqavr caefhphgkl yavgsnsktl ricsypklhd vredhqtyqp tvlfkrtkhh
      721 kgsiyclawt pdgqlmatgs ndktvklmrf nadtsnlegq eveltmhdgt vrdlcfledt
      781 snkssllisg gagdckiyvt dcatgtpfqa lsghsghvlt lynwggamfv sgsqdktvrf
      841 wdlrtrgcvn mvtpatvpgs rhliplqvgs pvaalcvdps grllvsghed sscvlfdirg
      901 grtvqcfkph aadirsirfs psayylltgg ydnklvltdl qgdltmplps vvvaqhqdkv
      961 isgrwhptef sflstsadkt atlwalppv
//
LOCUS       XP_076546479             983 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 47 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546479
VERSION     XP_076546479.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690364.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..983
                     /product="WD repeat-containing protein 47 isoform X2"
                     /calculated_mol_wt=108494
     Region          74..123
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     Region          <668..981
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          671..719
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          725..766
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          771..812
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          820..858
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          866..902
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          908..947
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          954..978
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..983
                     /gene="LOC117608649"
                     /coded_by="XM_076690364.1:594..3545"
                     /db_xref="GeneID:117608649"
ORIGIN      
        1 mvvvassait viedfskdtl grlriryvmp aahltlreed vvrltleflh srdlhisqls
       61 leretgving qysddvlflr qlildgqwdd vlefiqplea lpdfdmrkft ysilrhkyve
      121 llcikseanl natgtngsvd naveevvkvl sdlekvapsk eeysslclll tlprltdhlq
      181 ykdwnpsnar vqcfrevhll vekflpgdrk stdpahpvss akndrliqli ikgilyescv
      241 nycqakatgs keseqvemnf srlldgsvgf sdsdlsllsw lqsippetfa vpfaqrtlnv
      301 dverlerpsl etswtehmli tpikpktfph campftrprs aadmmsrslv paleaglgpr
      361 spgpkntpip saalmalstg dinpmsrssf asfhltgfkn nklmntsvdr lfenegdvfl
      421 sssyaeyqql psiqetttns qpkapprtrg qskspegika dpsaastper riagrespap
      481 starssrrds laekptgvaa kitepsvipg rpsiggdhle qsyngdlfke yqkqkqrlqe
      541 kfqqrerewd dlvrqlsapl spqvvdnrlq ndgikqplgs kgpspvhtst parsgiqspk
      601 pvingsepmv rqnsalgnaf dprvqeshyd vvkpikqepk qeldasngss nggrprfvpv
      661 taledvqavr caefhphgkl yavgsnsktl ricsypklhd vredhqtyqp tvlfkrtkhh
      721 kgsiyclawt pdgqlmatgs ndktvklmrf nadtsnlegq eveltmhdgt vrdlcfledt
      781 snkssllisg gagdckiyvt dcatgtpfqa lsghsghvlt lynwggamfv sgsqdktvrf
      841 wdlrtrgcvn mvtpatvpgs rvgspvaalc vdpsgrllvs ghedsscvlf dirggrtvqc
      901 fkphaadirs irfspsayyl ltggydnklv ltdlqgdltm plpsvvvaqh qdkvisgrwh
      961 ptefsflsts adktatlwal ppv
//
LOCUS       XP_034189986             979 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 47 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034189986
VERSION     XP_034189986.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334095.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034189986.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..979
                     /product="WD repeat-containing protein 47 isoform X3"
                     /calculated_mol_wt=108322
     Region          64..113
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     Region          <658..977
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          661..709
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          715..756
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          761..802
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          810..848
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          862..898
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          904..943
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          950..974
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..979
                     /gene="LOC117608649"
                     /coded_by="XM_034334095.2:100..3039"
                     /db_xref="GeneID:117608649"
ORIGIN      
        1 mskvckrtid rklriryvmp aahltlreed vvrltleflh srdlhisqls leretgving
       61 qysddvlflr qlildgqwdd vlefiqplea lpdfdmrkft ysilrhkyve llcikseanl
      121 natgtngsvd naveevvkvl sdlekvapsk eeysslclll tlprltdhlq ykdwnpsnar
      181 vqcfrevhll vekflpgdrk stdpahpvss akndrliqli ikgilyescv nycqakatgs
      241 keseqvemnf srlldgsvgf sdsdlsllsw lqsippetfa vpfaqrtlnv dverlerpsl
      301 etswtehmli tpikpktfph campftrprs aadmmsrslv paleaglgpr spgpkntpip
      361 saalmalstg dinpmsrssf asfhltgfkn nklmntsvdr lfenegdvfl sssyaeyqql
      421 psiqetttns qpkapprtrg qskspegika dpsaastper riagrespap starssrrds
      481 laekptgvaa kitepsvipg rpsiggdhle qsyngdlfke yqkqkqrlqe kfqqrerewd
      541 dlvrqlsapl spqvvdnrlq ndgikqplgs kgpspvhtst parsgiqspk pvingsepmv
      601 rqnsalgnaf dprvqeshyd vvkpikqepk qeldasngss nggrprfvpv taledvqavr
      661 caefhphgkl yavgsnsktl ricsypklhd vredhqtyqp tvlfkrtkhh kgsiyclawt
      721 pdgqlmatgs ndktvklmrf nadtsnlegq eveltmhdgt vrdlcfledt snkssllisg
      781 gagdckiyvt dcatgtpfqa lsghsghvlt lynwggamfv sgsqdktvrf wdlrtrgcvn
      841 mvtpatvpgs rhliplqvgs pvaalcvdps grllvsghed sscvlfdirg grtvqcfkph
      901 aadirsirfs psayylltgg ydnklvltdl qgdltmplps vvvaqhqdkv isgrwhptef
      961 sflstsadkt atlwalppv
//
LOCUS       XP_034190004             961 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 47 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190004
VERSION     XP_034190004.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334113.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190004.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..961
                     /product="WD repeat-containing protein 47 isoform X4"
                     /calculated_mol_wt=106075
     Region          46..95
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     Region          <640..959
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          643..691
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          697..738
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          743..784
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          792..830
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          844..880
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          886..925
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          932..956
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..961
                     /gene="LOC117608649"
                     /coded_by="XM_034334113.2:320..3205"
                     /db_xref="GeneID:117608649"
ORIGIN      
        1 mpaahltlre edvvrltlef lhsrdlhisq lsleretgvi ngqysddvlf lrqlildgqw
       61 ddvlefiqpl ealpdfdmrk ftysilrhky vellciksea nlnatgtngs vdnaveevvk
      121 vlsdlekvap skeeysslcl lltlprltdh lqykdwnpsn arvqcfrevh llvekflpgd
      181 rkstdpahpv ssakndrliq liikgilyes cvnycqakat gskeseqvem nfsrlldgsv
      241 gfsdsdlsll swlqsippet favpfaqrtl nvdverlerp sletswtehm litpikpktf
      301 phcampftrp rsaadmmsrs lvpaleaglg prspgpkntp ipsaalmals tgdinpmsrs
      361 sfasfhltgf knnklmntsv drlfenegdv flsssyaeyq qlpsiqettt nsqpkapprt
      421 rgqskspegi kadpsaastp erriagresp apstarssrr dslaekptgv aakitepsvi
      481 pgrpsiggdh leqsyngdlf keyqkqkqrl qekfqqrere wddlvrqlsa plspqvvdnr
      541 lqndgikqpl gskgpspvht stparsgiqs pkpvingsep mvrqnsalgn afdprvqesh
      601 ydvvkpikqe pkqeldasng ssnggrprfv pvtaledvqa vrcaefhphg klyavgsnsk
      661 tlricsypkl hdvredhqty qptvlfkrtk hhkgsiycla wtpdgqlmat gsndktvklm
      721 rfnadtsnle gqeveltmhd gtvrdlcfle dtsnksslli sggagdckiy vtdcatgtpf
      781 qalsghsghv ltlynwggam fvsgsqdktv rfwdlrtrgc vnmvtpatvp gsrhliplqv
      841 gspvaalcvd psgrllvsgh edsscvlfdi rggrtvqcfk phaadirsir fspsayyllt
      901 ggydnklvlt dlqgdltmpl psvvvaqhqd kvisgrwhpt efsflstsad ktatlwalpp
      961 v
//
LOCUS       XP_034190028             893 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608673 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190028
VERSION     XP_034190028.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190028.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..893
                     /product="uncharacterized protein LOC117608673 isoform X1"
                     /calculated_mol_wt=99023
     Region          240..>332
                     /region_name="LRR_5"
                     /note="BspA type Leucine rich repeat region (6 copies);
                     pfam13306"
                     /db_xref="CDD:463839"
     Region          <676..893
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..893
                     /gene="LOC117608673"
                     /coded_by="XM_034334137.2:458..3139"
                     /db_xref="GeneID:117608673"
ORIGIN      
        1 mnarrrvfsg ldglkcdrai vfsvflwclv flsggvesap pgicamdgcn ctvkahrwin
       61 vkcvfgndqd vellegtips dvmevevshc relriqsgaf adgaplkrvh vsgiyslvan
      121 rqaflnlsap nthlevsecn svileshafr nskgtlsvsi srcrhvgikp nafsrllwit
      181 vrevptlels snafkleasq hgrhgpatki mfqsvritel ptavfpsava eirmddiwtk
      241 virknafcam tifsvtisna siqaietgaf sdraliqsle fvdvrlksie kgafraghdn
      301 ltiqysrlse vdtgaidisa atvsfnnnef qslhqgaivl hqwnhiaidy nlfvdletda
      361 itaevdatsa pnfefsftgn hiekarpgsl kfaaisqrvn tarvgnnyfk ekcscnldsw
      421 irevtgknss vswmmdssyc vvdqlldrcf nlpqgymsmr nftkiicmpg dhiscekpsa
      481 kpepsvspps vgphvyprhk gyfdvemsds eqlqrekrii vvicvlavli vlvvilssgv
      541 lymrrngvcp kltsghlmnl aswlspssgm taatsarsis rlsvheyagl qpetrileve
      601 tqpevaeeee edaeglypyt enkatqtlpe elteeylrdl rerlndpdny nqardmiehl
      661 ydlikveesc nnnndrqptg reenaydviq pkqrkhrgpp kpsvsvgtka pslekllpsg
      721 iqprppltey teprdqrpne qnhlyaelpg detvpstsrl sqpilatlag rapqplppdv
      781 vndhlindhs sqafgslkte klrppdspkf elnrnhgrpm sflkalgesi lgssktnnkr
      841 pnsllceyae psdatahlys elpepqtstp agkmanrplp tkpdqdsvnl trs
//
LOCUS       XP_076546480             835 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608673 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546480
VERSION     XP_076546480.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690365.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..835
                     /product="uncharacterized protein LOC117608673 isoform X2"
                     /calculated_mol_wt=92906
     Region          182..>274
                     /region_name="LRR_5"
                     /note="BspA type Leucine rich repeat region (6 copies);
                     pfam13306"
                     /db_xref="CDD:463839"
     Region          <618..835
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..835
                     /gene="LOC117608673"
                     /coded_by="XM_076690365.1:191..2698"
                     /db_xref="GeneID:117608673"
ORIGIN      
        1 mesfwprner ldvellegti psdvmevevs hcrelriqsg afadgaplkr vhvsgiyslv
       61 anrqaflnls apnthlevse cnsvilesha frnskgtlsv sisrcrhvgi kpnafsrllw
      121 itvrevptle lssnafklea sqhgrhgpat kimfqsvrit elptavfpsa vaeirmddiw
      181 tkvirknafc amtifsvtis nasiqaietg afsdraliqs lefvdvrlks iekgafragh
      241 dnltiqysrl sevdtgaidi saatvsfnnn efqslhqgai vlhqwnhiai dynlfvdlet
      301 daitaevdat sapnfefsft gnhiekarpg slkfaaisqr vntarvgnny fkekcscnld
      361 swirevtgkn ssvswmmdss ycvvdqlldr cfnlpqgyms mrnftkiicm pgdhiscekp
      421 sakpepsvsp psvgphvypr hkgyfdvems dseqlqrekr iivvicvlav livlvvilss
      481 gvlymrrngv cpkltsghlm nlaswlspss gmtaatsars isrlsvheya glqpetrile
      541 vetqpevaee eeedaeglyp ytenkatqtl peelteeylr dlrerlndpd nynqardmie
      601 hlydlikvee scnnnndrqp tgreenaydv iqpkqrkhrg ppkpsvsvgt kapslekllp
      661 sgiqprpplt eyteprdqrp neqnhlyael pgdetvpsts rlsqpilatl agrapqplpp
      721 dvvndhlind hssqafgslk teklrppdsp kfelnrnhgr pmsflkalge silgssktnn
      781 krpnsllcey aepsdatahl yselpepqts tpagkmanrp lptkpdqdsv nltrs
//
LOCUS       XP_034190041             850 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190041
VERSION     XP_034190041.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334150.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..850
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1 isoform X1"
                     /calculated_mol_wt=96891
     Region          107..135
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          155..178
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(169..170,174,177..178,209..210,213,216..217,220,
                     248..249,252,255..256,259,287..288,291,294..295,326..327,
                     330,333)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          193..220
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          230..260
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          269..295
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          306..333
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          566..591
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(618..619,622,625..626,659..660,663,666..667,670,
                     698..699,702,705..706,709,739..740,747,750..751,781..782,
                     785,788..789)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          638..670
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          680..710
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          682..826
                     /region_name="HEAT"
                     /note="HEAT repeat [General function prediction only];
                     COG1413"
                     /db_xref="CDD:441023"
     Region          716..751
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          761..789
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..850
                     /gene="LOC117608677"
                     /coded_by="XM_034334150.2:366..2918"
                     /db_xref="GeneID:117608677"
ORIGIN      
        1 mllerlcldl hpqstsddip rqltnsmeid evdeddvdyn lspnasgtgi csvggsgdea
       61 grdelllklq pyvtsdtnft rqrigrilve tfrtaanngt klnvneimqs vqqiiqdpdt
      121 qvrldlmeql phvaticqea shlfgdvlhd hlfsiiityl qdqdnqvrmt tqavvltlik
      181 sglldsttie vkvcpvievl crtsvdflqs gimfmskmap ligkklteki fldryialck
      241 eqdihvrkvc ashfgeicva vrrkaflqkl fpvfvdlccd kawgvrkacv dvmmpvaccm
      301 tlqqrqllla ellamhldde skwvrmsafq ilgpfistfa kqftevtyne hgelvftsqq
      361 dtrfsirysy egnyppkyvv rshsfdqeel dtkdnfnspv iiqkpisdde eeedsqedhi
      421 falraykskm qrkmnqnqst ddtekyntfl yyyiapdlpl deelveaakr ssaknnvdwk
      481 pmadtasska sslddvveee kfpevelitv neeelvplhl vnvfltmaep erysdigadi
      541 lhhcafsfpa valtlgrehw cylqptykll lnadqwkvrr tlassiheia tilgeeltas
      601 dlipvydwfi kdvdevrigv lkhlatflki lkpsdrclyl prlkeflvtd newnwrfree
      661 latqlleivn lfgatdvery ivplslells dkvaavrhva lslvtqivah lsdnerlita
      721 lfqelkfslg vwtkkwirrq tlvfvcaqli annaisghrf sqemlpsllk lsrdkvpnvr
      781 lavartlskn vipmgpnwlg aeqaeevekr lremrsdpdr dvrvlaggee npvldilsqt
      841 kaeqsrnilf
//
LOCUS       XP_034190048             824 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190048
VERSION     XP_034190048.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334157.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..824
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1 isoform X2"
                     /calculated_mol_wt=93912
     Region          81..109
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          129..152
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(143..144,148,151..152,183..184,187,190..191,194,
                     222..223,226,229..230,233,261..262,265,268..269,300..301,
                     304,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          167..194
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          204..234
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          243..269
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          280..307
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          540..565
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(592..593,596,599..600,633..634,637,640..641,644,
                     672..673,676,679..680,683,713..714,721,724..725,755..756,
                     759,762..763)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          612..644
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          654..684
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          656..800
                     /region_name="HEAT"
                     /note="HEAT repeat [General function prediction only];
                     COG1413"
                     /db_xref="CDD:441023"
     Region          690..725
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          735..763
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..824
                     /gene="LOC117608677"
                     /coded_by="XM_034334157.2:132..2606"
                     /db_xref="GeneID:117608677"
ORIGIN      
        1 meidevdedd vdynlspnas gtgicsvggs gdeagrdell lklqpyvtsd tnftrqrigr
       61 ilvetfrtaa nngtklnvne imqsvqqiiq dpdtqvrldl meqlphvati cqeashlfgd
      121 vlhdhlfsii itylqdqdnq vrmttqavvl tliksgllds ttievkvcpv ievlcrtsvd
      181 flqsgimfms kmapligkkl tekifldryi alckeqdihv rkvcashfge icvavrrkaf
      241 lqklfpvfvd lccdkawgvr kacvdvmmpv accmtlqqrq lllaellamh lddeskwvrm
      301 safqilgpfi stfakqftev tynehgelvf tsqqdtrfsi rysyegnypp kyvvrshsfd
      361 qeeldtkdnf nspviiqkpi sddeeeedsq edhifalray kskmqrkmnq nqstddteky
      421 ntflyyyiap dlpldeelve aakrssaknn vdwkpmadta sskasslddv veeekfpeve
      481 litvneeelv plhlvnvflt maeperysdi gadilhhcaf sfpavaltlg rehwcylqpt
      541 yklllnadqw kvrrtlassi heiatilgee ltasdlipvy dwfikdvdev rigvlkhlat
      601 flkilkpsdr clylprlkef lvtdnewnwr freelatqll eivnlfgatd veryivplsl
      661 ellsdkvaav rhvalslvtq ivahlsdner litalfqelk fslgvwtkkw irrqtlvfvc
      721 aqliannais ghrfsqemlp sllklsrdkv pnvrlavart lsknvipmgp nwlgaeqaee
      781 vekrlremrs dpdrdvrvla ggeenpvldi lsqtkaeqsr nilf
//
LOCUS       XP_034190058             824 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190058
VERSION     XP_034190058.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334167.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..824
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1 isoform X2"
                     /calculated_mol_wt=93912
     Region          81..109
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          129..152
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(143..144,148,151..152,183..184,187,190..191,194,
                     222..223,226,229..230,233,261..262,265,268..269,300..301,
                     304,307)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          167..194
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          204..234
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          243..269
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          280..307
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          540..565
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(592..593,596,599..600,633..634,637,640..641,644,
                     672..673,676,679..680,683,713..714,721,724..725,755..756,
                     759,762..763)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          612..644
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          654..684
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          656..800
                     /region_name="HEAT"
                     /note="HEAT repeat [General function prediction only];
                     COG1413"
                     /db_xref="CDD:441023"
     Region          690..725
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          735..763
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..824
                     /gene="LOC117608677"
                     /coded_by="XM_034334167.2:1090..3564"
                     /db_xref="GeneID:117608677"
ORIGIN      
        1 meidevdedd vdynlspnas gtgicsvggs gdeagrdell lklqpyvtsd tnftrqrigr
       61 ilvetfrtaa nngtklnvne imqsvqqiiq dpdtqvrldl meqlphvati cqeashlfgd
      121 vlhdhlfsii itylqdqdnq vrmttqavvl tliksgllds ttievkvcpv ievlcrtsvd
      181 flqsgimfms kmapligkkl tekifldryi alckeqdihv rkvcashfge icvavrrkaf
      241 lqklfpvfvd lccdkawgvr kacvdvmmpv accmtlqqrq lllaellamh lddeskwvrm
      301 safqilgpfi stfakqftev tynehgelvf tsqqdtrfsi rysyegnypp kyvvrshsfd
      361 qeeldtkdnf nspviiqkpi sddeeeedsq edhifalray kskmqrkmnq nqstddteky
      421 ntflyyyiap dlpldeelve aakrssaknn vdwkpmadta sskasslddv veeekfpeve
      481 litvneeelv plhlvnvflt maeperysdi gadilhhcaf sfpavaltlg rehwcylqpt
      541 yklllnadqw kvrrtlassi heiatilgee ltasdlipvy dwfikdvdev rigvlkhlat
      601 flkilkpsdr clylprlkef lvtdnewnwr freelatqll eivnlfgatd veryivplsl
      661 ellsdkvaav rhvalslvtq ivahlsdner litalfqelk fslgvwtkkw irrqtlvfvc
      721 aqliannais ghrfsqemlp sllklsrdkv pnvrlavart lsknvipmgp nwlgaeqaee
      781 vekrlremrs dpdrdvrvla ggeenpvldi lsqtkaeqsr nilf
//
LOCUS       XP_034190066             775 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform
            X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190066
VERSION     XP_034190066.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334175.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..775
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1 isoform X3"
                     /calculated_mol_wt=88824
     Region          32..60
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          80..103
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(94..95,99,102..103,134..135,138,141..142,145,
                     173..174,177,180..181,184,212..213,216,219..220,251..252,
                     255,258)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          118..145
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          155..185
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          194..220
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          231..258
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          491..516
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(543..544,547,550..551,584..585,588,591..592,595,
                     623..624,627,630..631,634,664..665,672,675..676,706..707,
                     710,713..714)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          563..595
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          605..635
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          607..751
                     /region_name="HEAT"
                     /note="HEAT repeat [General function prediction only];
                     COG1413"
                     /db_xref="CDD:441023"
     Region          641..676
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          686..714
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..775
                     /gene="LOC117608677"
                     /coded_by="XM_034334175.2:16..2343"
                     /db_xref="GeneID:117608677"
ORIGIN      
        1 mrgrfrqrig rilvetfrta anngtklnvn eimqsvqqii qdpdtqvrld lmeqlphvat
       61 icqeashlfg dvlhdhlfsi iitylqdqdn qvrmttqavv ltliksglld sttievkvcp
      121 vievlcrtsv dflqsgimfm skmapligkk ltekifldry ialckeqdih vrkvcashfg
      181 eicvavrrka flqklfpvfv dlccdkawgv rkacvdvmmp vaccmtlqqr qlllaellam
      241 hlddeskwvr msafqilgpf istfakqfte vtynehgelv ftsqqdtrfs irysyegnyp
      301 pkyvvrshsf dqeeldtkdn fnspviiqkp isddeeeeds qedhifalra ykskmqrkmn
      361 qnqstddtek yntflyyyia pdlpldeelv eaakrssakn nvdwkpmadt asskassldd
      421 vveeekfpev elitvneeel vplhlvnvfl tmaeperysd igadilhhca fsfpavaltl
      481 grehwcylqp tyklllnadq wkvrrtlass iheiatilge eltasdlipv ydwfikdvde
      541 vrigvlkhla tflkilkpsd rclylprlke flvtdnewnw rfreelatql leivnlfgat
      601 dveryivpls lellsdkvaa vrhvalslvt qivahlsdne rlitalfqel kfslgvwtkk
      661 wirrqtlvfv caqliannai sghrfsqeml psllklsrdk vpnvrlavar tlsknvipmg
      721 pnwlgaeqae evekrlremr sdpdrdvrvl aggeenpvld ilsqtkaeqs rnilf
//
LOCUS       XP_034190086             743 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform
            X4 [Osmia lignaria lignaria].
ACCESSION   XP_034190086
VERSION     XP_034190086.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334195.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..743
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1 isoform X4"
                     /calculated_mol_wt=85109
     Region          1..28
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          48..71
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(62..63,67,70..71,102..103,106,109..110,113,141..142,
                     145,148..149,152,180..181,184,187..188,219..220,223,226)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          86..113
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          123..153
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          162..188
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          199..226
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(511..512,515,518..519,552..553,556,559..560,563,
                     591..592,595,598..599,602,632..633,640,643..644,674..675,
                     678,681..682)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          531..563
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          573..603
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          575..719
                     /region_name="HEAT"
                     /note="HEAT repeat [General function prediction only];
                     COG1413"
                     /db_xref="CDD:441023"
     Region          609..644
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          654..682
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..743
                     /gene="LOC117608677"
                     /coded_by="XM_034334195.2:1121..3352"
                     /db_xref="GeneID:117608677"
ORIGIN      
        1 mqsvqqiiqd pdtqvrldlm eqlphvatic qeashlfgdv lhdhlfsiii tylqdqdnqv
       61 rmttqavvlt liksglldst tievkvcpvi evlcrtsvdf lqsgimfmsk mapligkklt
      121 ekifldryia lckeqdihvr kvcashfgei cvavrrkafl qklfpvfvdl ccdkawgvrk
      181 acvdvmmpva ccmtlqqrql llaellamhl ddeskwvrms afqilgpfis tfakqftevt
      241 ynehgelvft sqqdtrfsir ysyegnyppk yvvrshsfdq eeldtkdnfn spviiqkpis
      301 ddeeeedsqe dhifalrayk skmqrkmnqn qstddtekyn tflyyyiapd lpldeelvea
      361 akrssaknnv dwkpmadtas skasslddvv eeekfpevel itvneeelvp lhlvnvfltm
      421 aeperysdig adilhhcafs fpavaltlgr ehwcylqpty klllnadqwk vrrtlassih
      481 eiatilgeel tasdlipvyd wfikdvdevr igvlkhlatf lkilkpsdrc lylprlkefl
      541 vtdnewnwrf reelatqlle ivnlfgatdv eryivplsle llsdkvaavr hvalslvtqi
      601 vahlsdnerl italfqelkf slgvwtkkwi rrqtlvfvca qliannaisg hrfsqemlps
      661 llklsrdkvp nvrlavartl sknvipmgpn wlgaeqaeev ekrlremrsd pdrdvrvlag
      721 geenpvldil sqtkaeqsrn ilf
//
LOCUS       XP_034190096             697 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase 4 regulatory subunit 1 isoform
            X5 [Osmia lignaria lignaria].
ACCESSION   XP_034190096
VERSION     XP_034190096.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334205.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="serine/threonine-protein phosphatase 4
                     regulatory subunit 1 isoform X5"
                     /calculated_mol_wt=79571
     Region          107..135
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          155..178
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(169..170,174,177..178,209..210,213,216..217,220,
                     248..249,252,255..256,259,287..288,291,294..295,326..327,
                     330,333)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          193..220
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          230..260
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          269..295
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          306..333
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          602..630
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          640..671
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..697
                     /gene="LOC117608677"
                     /coded_by="XM_034334205.2:374..2467"
                     /db_xref="GeneID:117608677"
ORIGIN      
        1 mllerlcldl hpqstsddip rqltnsmeid evdeddvdyn lspnasgtgi csvggsgdea
       61 grdelllklq pyvtsdtnft rqrigrilve tfrtaanngt klnvneimqs vqqiiqdpdt
      121 qvrldlmeql phvaticqea shlfgdvlhd hlfsiiityl qdqdnqvrmt tqavvltlik
      181 sglldsttie vkvcpvievl crtsvdflqs gimfmskmap ligkklteki fldryialck
      241 eqdihvrkvc ashfgeicva vrrkaflqkl fpvfvdlccd kawgvrkacv dvmmpvaccm
      301 tlqqrqllla ellamhldde skwvrmsafq ilgpfistfa kqftevtyne hgelvftsqq
      361 dtrfsirysy egnyppkyvv rshsfdqeel dtkdnfnspv iiqkpisdde eeedsqedhi
      421 falraykskm qrkmnqnqst ddtekyntfl yyyiapdlpl deelveaakr ssaknnvdwk
      481 pmadtasska sslddvveee kfpevelitv neeelvplhl vnvfltmaep erysdigadi
      541 lhhcafsfpa valtlgrehw cylqptykll lnadqwkvrr tlassiheia tilgeeltas
      601 dlipvydwfi kdvdevrigv lkhlatflki lkpsdrclyl prlkeflvtd newnwrfree
      661 latqlleivn lfgatdvery ivplslells dkvaavr
//
LOCUS       XP_034190109             532 aa            linear   INV 03-SEP-2025
DEFINITION  RING finger and SPRY domain-containing protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190109
VERSION     XP_034190109.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334218.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..532
                     /product="RING finger and SPRY domain-containing protein
                     1"
                     /calculated_mol_wt=59556
     Region          316..437
                     /region_name="SPRY_RING"
                     /note="SPRY domain at N-terminus of Really Interesting New
                     Gene (RING) finger domain; cd12883"
                     /db_xref="CDD:293941"
     Region          482..524
                     /region_name="RING-HC_RSPRY1"
                     /note="RING finger, HC subclass, found in RING finger and
                     SPRY domain-containing protein 1 (RSPRY1) and similar
                     proteins; cd16566"
                     /db_xref="CDD:438228"
     CDS             1..532
                     /gene="LOC117608698"
                     /coded_by="XM_034334218.2:732..2330"
                     /db_xref="GeneID:117608698"
ORIGIN      
        1 mgnclckdpl edfdiyrgqn hagtentvlp esgvdtvasg dvtstpfkfp tsanidklvl
       61 etlgvigslv dneqepppam lklhaiadke dgwiqvvssm vnvipmhnpl gpsvitllld
      121 dcplpskesv lrlshmfqls qklgniqtsa tqqrnicvvl gciaeklagp ssiailseat
      181 ldylisnlke nidayvvlys lialekfaqt senkvtikkr lmaekpnpll elekwateth
      241 yvrrqvgfca qwcldnlflm egrkyshdav dmsginvmln tkdvseylki spnglearcd
      301 aysfesvrct fqvdsgiwyy etlvittgvm qigwatkdst flnhegygig ddefslaydg
      361 crrliwynak sekyhekpcw kagdilgcll dlnkleiifs ingvplkpcv qvfktvrsgf
      421 faaasfmsfq qclfnfgnvp fkypptdrey kkfndfaslk pedkvvlprh iyldqlrkls
      481 vredsctlcf dqrasvrllp cnhrgfcqtc snqlvecpmc ratieevald nt
//
LOCUS       XP_034190118             532 aa            linear   INV 03-SEP-2025
DEFINITION  RING finger and SPRY domain-containing protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190118
VERSION     XP_034190118.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334227.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..532
                     /product="RING finger and SPRY domain-containing protein
                     1"
                     /calculated_mol_wt=59556
     Region          316..437
                     /region_name="SPRY_RING"
                     /note="SPRY domain at N-terminus of Really Interesting New
                     Gene (RING) finger domain; cd12883"
                     /db_xref="CDD:293941"
     Region          482..524
                     /region_name="RING-HC_RSPRY1"
                     /note="RING finger, HC subclass, found in RING finger and
                     SPRY domain-containing protein 1 (RSPRY1) and similar
                     proteins; cd16566"
                     /db_xref="CDD:438228"
     CDS             1..532
                     /gene="LOC117608698"
                     /coded_by="XM_034334227.2:427..2025"
                     /db_xref="GeneID:117608698"
ORIGIN      
        1 mgnclckdpl edfdiyrgqn hagtentvlp esgvdtvasg dvtstpfkfp tsanidklvl
       61 etlgvigslv dneqepppam lklhaiadke dgwiqvvssm vnvipmhnpl gpsvitllld
      121 dcplpskesv lrlshmfqls qklgniqtsa tqqrnicvvl gciaeklagp ssiailseat
      181 ldylisnlke nidayvvlys lialekfaqt senkvtikkr lmaekpnpll elekwateth
      241 yvrrqvgfca qwcldnlflm egrkyshdav dmsginvmln tkdvseylki spnglearcd
      301 aysfesvrct fqvdsgiwyy etlvittgvm qigwatkdst flnhegygig ddefslaydg
      361 crrliwynak sekyhekpcw kagdilgcll dlnkleiifs ingvplkpcv qvfktvrsgf
      421 faaasfmsfq qclfnfgnvp fkypptdrey kkfndfaslk pedkvvlprh iyldqlrkls
      481 vredsctlcf dqrasvrllp cnhrgfcqtc snqlvecpmc ratieevald nt
//
LOCUS       XP_034190130             507 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608710 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190130
VERSION     XP_034190130.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334239.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="uncharacterized protein LOC117608710 isoform X1"
                     /calculated_mol_wt=56107
     CDS             1..507
                     /gene="LOC117608710"
                     /coded_by="XM_034334239.2:726..2249"
                     /db_xref="GeneID:117608710"
ORIGIN      
        1 mgatkfllgs avlgllwscl fkgaltnsts rcdyppcycd hhgrltcdck degeelvltt
       61 egerklspht srivvgncss vilsnsslas maglrsvdli nvanlslvrq sfelsprstr
      121 trisvrncsi dsmpsfvfrg dveaisfenv rigqlsafsf anllhtdtlr lencriesme
      181 aqafkkfdvg ylhviggsfg dqvpsrtmnd ievyhkfmld gvkmgtvrse afivrsprtv
      241 aiqncamesl eseafdvtar gavilknnsf gslavgaflg iradpeartv slplnlhdli
      301 fknnsvvnfe egsmmfdrss frleldnllv ekpcdcqmlp vwknnvlnyt nvytrfysrq
      361 nsvlpptmtp qdsinetpet flcydgevdg vpigfveydt rhcslggsil vfilivagll
      421 vflivlacli vwccrrrrrn grkkwisvpt napdvvskkn gvigretaas aapvdsritm
      481 vvpdgrlyre tefhviveka eplttel
//
LOCUS       XP_034190138             507 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608710 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190138
VERSION     XP_034190138.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334247.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="uncharacterized protein LOC117608710 isoform X1"
                     /calculated_mol_wt=56107
     CDS             1..507
                     /gene="LOC117608710"
                     /coded_by="XM_034334247.2:670..2193"
                     /db_xref="GeneID:117608710"
ORIGIN      
        1 mgatkfllgs avlgllwscl fkgaltnsts rcdyppcycd hhgrltcdck degeelvltt
       61 egerklspht srivvgncss vilsnsslas maglrsvdli nvanlslvrq sfelsprstr
      121 trisvrncsi dsmpsfvfrg dveaisfenv rigqlsafsf anllhtdtlr lencriesme
      181 aqafkkfdvg ylhviggsfg dqvpsrtmnd ievyhkfmld gvkmgtvrse afivrsprtv
      241 aiqncamesl eseafdvtar gavilknnsf gslavgaflg iradpeartv slplnlhdli
      301 fknnsvvnfe egsmmfdrss frleldnllv ekpcdcqmlp vwknnvlnyt nvytrfysrq
      361 nsvlpptmtp qdsinetpet flcydgevdg vpigfveydt rhcslggsil vfilivagll
      421 vflivlacli vwccrrrrrn grkkwisvpt napdvvskkn gvigretaas aapvdsritm
      481 vvpdgrlyre tefhviveka eplttel
//
LOCUS       XP_034190146             507 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608710 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190146
VERSION     XP_034190146.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334255.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="uncharacterized protein LOC117608710 isoform X1"
                     /calculated_mol_wt=56107
     CDS             1..507
                     /gene="LOC117608710"
                     /coded_by="XM_034334255.2:1058..2581"
                     /db_xref="GeneID:117608710"
ORIGIN      
        1 mgatkfllgs avlgllwscl fkgaltnsts rcdyppcycd hhgrltcdck degeelvltt
       61 egerklspht srivvgncss vilsnsslas maglrsvdli nvanlslvrq sfelsprstr
      121 trisvrncsi dsmpsfvfrg dveaisfenv rigqlsafsf anllhtdtlr lencriesme
      181 aqafkkfdvg ylhviggsfg dqvpsrtmnd ievyhkfmld gvkmgtvrse afivrsprtv
      241 aiqncamesl eseafdvtar gavilknnsf gslavgaflg iradpeartv slplnlhdli
      301 fknnsvvnfe egsmmfdrss frleldnllv ekpcdcqmlp vwknnvlnyt nvytrfysrq
      361 nsvlpptmtp qdsinetpet flcydgevdg vpigfveydt rhcslggsil vfilivagll
      421 vflivlacli vwccrrrrrn grkkwisvpt napdvvskkn gvigretaas aapvdsritm
      481 vvpdgrlyre tefhviveka eplttel
//
LOCUS       XP_034190155             507 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608710 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190155
VERSION     XP_034190155.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334264.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="uncharacterized protein LOC117608710 isoform X1"
                     /calculated_mol_wt=56107
     CDS             1..507
                     /gene="LOC117608710"
                     /coded_by="XM_034334264.2:1351..2874"
                     /db_xref="GeneID:117608710"
ORIGIN      
        1 mgatkfllgs avlgllwscl fkgaltnsts rcdyppcycd hhgrltcdck degeelvltt
       61 egerklspht srivvgncss vilsnsslas maglrsvdli nvanlslvrq sfelsprstr
      121 trisvrncsi dsmpsfvfrg dveaisfenv rigqlsafsf anllhtdtlr lencriesme
      181 aqafkkfdvg ylhviggsfg dqvpsrtmnd ievyhkfmld gvkmgtvrse afivrsprtv
      241 aiqncamesl eseafdvtar gavilknnsf gslavgaflg iradpeartv slplnlhdli
      301 fknnsvvnfe egsmmfdrss frleldnllv ekpcdcqmlp vwknnvlnyt nvytrfysrq
      361 nsvlpptmtp qdsinetpet flcydgevdg vpigfveydt rhcslggsil vfilivagll
      421 vflivlacli vwccrrrrrn grkkwisvpt napdvvskkn gvigretaas aapvdsritm
      481 vvpdgrlyre tefhviveka eplttel
//
LOCUS       XP_034190165             454 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608710 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190165
VERSION     XP_034190165.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334274.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..454
                     /product="uncharacterized protein LOC117608710 isoform X2"
                     /calculated_mol_wt=50370
     CDS             1..454
                     /gene="LOC117608710"
                     /coded_by="XM_034334274.2:479..1843"
                     /db_xref="GeneID:117608710"
ORIGIN      
        1 melvlttege rklsphtsri vvgncssvil snsslasmag lrsvdlinva nlslvrqsfe
       61 lsprstrtri svrncsidsm psfvfrgdve aisfenvrig qlsafsfanl lhtdtlrlen
      121 criesmeaqa fkkfdvgylh viggsfgdqv psrtmndiev yhkfmldgvk mgtvrseafi
      181 vrsprtvaiq ncameslese afdvtargav ilknnsfgsl avgaflgira dpeartvslp
      241 lnlhdlifkn nsvvnfeegs mmfdrssfrl eldnllvekp cdcqmlpvwk nnvlnytnvy
      301 trfysrqnsv lpptmtpqds inetpetflc ydgevdgvpi gfveydtrhc slggsilvfi
      361 livagllvfl ivlaclivwc crrrrrngrk kwisvptnap dvvskkngvi gretaasaap
      421 vdsritmvvp dgrlyretef hvivekaepl ttel
//
LOCUS       XP_034190176             417 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608710 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190176
VERSION     XP_034190176.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334285.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..417
                     /product="uncharacterized protein LOC117608710 isoform X3"
                     /calculated_mol_wt=46472
     CDS             1..417
                     /gene="LOC117608710"
                     /coded_by="XM_034334285.2:247..1500"
                     /db_xref="GeneID:117608710"
ORIGIN      
        1 maglrsvdli nvanlslvrq sfelsprstr trisvrncsi dsmpsfvfrg dveaisfenv
       61 rigqlsafsf anllhtdtlr lencriesme aqafkkfdvg ylhviggsfg dqvpsrtmnd
      121 ievyhkfmld gvkmgtvrse afivrsprtv aiqncamesl eseafdvtar gavilknnsf
      181 gslavgaflg iradpeartv slplnlhdli fknnsvvnfe egsmmfdrss frleldnllv
      241 ekpcdcqmlp vwknnvlnyt nvytrfysrq nsvlpptmtp qdsinetpet flcydgevdg
      301 vpigfveydt rhcslggsil vfilivagll vflivlacli vwccrrrrrn grkkwisvpt
      361 napdvvskkn gvigretaas aapvdsritm vvpdgrlyre tefhviveka eplttel
//
LOCUS       XP_034190382            1047 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190382
VERSION     XP_034190382.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334491.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1047
                     /product="myosin binding subunit isoform X1"
                     /calculated_mol_wt=115883
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          671..713
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          948..1043
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..1047
                     /gene="Mbs"
                     /coded_by="XM_034334491.2:394..3537"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vaiciscnna awteatretd meesddeges etssdshsst ysnqsdksne snhspcltdd
      421 ekknranree tsprnlsspi evnkvpnqap vmppkqqtds neegvipswr rsgsfrnriq
      481 ntettntvst rleekdknii sptipnklnt etdvvltrth sfetdekfye qylalrarik
      541 afscptlhcc naatpthtnt ttrsaslret yrrkeaklnl elsrlpqgsn tlsptstskt
      601 ivsstlpttt atatttattt tttspiptnq irsvqpvegt ttnnsvavpg tpttpggskl
      661 spgnifknff ksfvppvrde esetqrkaha krvretrrst qgvtldeiks aeqlvkkkqq
      721 nneipttvss tqpatttsnt asitatitta tpttvttnks peelnlperr pswrlkvdng
      781 skfqledann rtatvpnpdt ttaymrrpst gtsiprpssa pvetiatssa ettvtlplrr
      841 plkqpedkeq dkendsrnaq atqaviqrrr rpkrrstgvv hvdmdeidpe kqdltaggdc
      901 denkinhesg ndrsgrsnrl gsisslsset psvsarikst tsengeldyk klyeesqaen
      961 erlkeklrrs ddqlkevrnl ldkaqsaqnk tvlseaekre rramerklse meeelkqlqk
     1021 lkaenerlka enraltrvvs kltnttk
//
LOCUS       XP_034190391            1044 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190391
VERSION     XP_034190391.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334500.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="myosin binding subunit isoform X2"
                     /calculated_mol_wt=115531
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          671..713
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          948..1044
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..1044
                     /gene="Mbs"
                     /coded_by="XM_034334500.2:394..3528"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vaiciscnna awteatretd meesddeges etssdshsst ysnqsdksne snhspcltdd
      421 ekknranree tsprnlsspi evnkvpnqap vmppkqqtds neegvipswr rsgsfrnriq
      481 ntettntvst rleekdknii sptipnklnt etdvvltrth sfetdekfye qylalrarik
      541 afscptlhcc naatpthtnt ttrsaslret yrrkeaklnl elsrlpqgsn tlsptstskt
      601 ivsstlpttt atatttattt tttspiptnq irsvqpvegt ttnnsvavpg tpttpggskl
      661 spgnifknff ksfvppvrde esetqrkaha krvretrrst qgvtldeiks aeqlvkkkqq
      721 nneipttvss tqpatttsnt asitatitta tpttvttnks peelnlperr pswrlkvdng
      781 skfqledann rtatvpnpdt ttaymrrpst gtsiprpssa pvetiatssa ettvtlplrr
      841 plkqpedkeq dkendsrnaq atqaviqrrr rpkrrstgvv hvdmdeidpe kqdltaggdc
      901 denkinhesg ndrsgrsnrl gsisslsset psvsarikst tsengeldyk klyeesqaen
      961 erlkeklrrs ddqlkevrnl ldkaqsaqnk tvlseaekre rramerklse meeelkvmdq
     1021 lkcenqrlkd engalirvis klsk
//
LOCUS       XP_034190400            1035 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190400
VERSION     XP_034190400.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334509.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1035
                     /product="myosin binding subunit isoform X3"
                     /calculated_mol_wt=114635
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          659..701
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          936..1031
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..1035
                     /gene="Mbs"
                     /coded_by="XM_034334509.2:393..3500"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 veatretdme esddegeset ssdshsstys nqsdksnesn hspcltddek knranreets
      421 prnlsspiev nkvpnqapvm ppkqqtdsne egvipswrrs gsfrnriqnt ettntvstrl
      481 eekdkniisp tipnklntet dvvltrthsf etdekfyeqy lalrarikaf scptlhccna
      541 atpthtnttt rsaslretyr rkeaklnlel srlpqgsntl sptstsktiv sstlptttat
      601 atttattttt tspiptnqir svqpvegttt nnsvavpgtp ttpggsklsp gnifknffks
      661 fvppvrdees etqrkahakr vretrrstqg vtldeiksae qlvkkkqqnn eipttvsstq
      721 patttsntas itatittatp ttvttnkspe elnlperrps wrlkvdngsk fqledannrt
      781 atvpnpdttt aymrrpstgt siprpssapv etiatssaet tvtlplrrpl kqpedkeqdk
      841 endsrnaqat qaviqrrrrp krrstgvvhv dmdeidpekq dltaggdcde nkinhesgnd
      901 rsgrsnrlgs isslssetps vsarikstts engeldykkl yeesqaener lkeklrrsdd
      961 qlkevrnlld kaqsaqnktv lseaekrerr amerklseme eelkqlqklk aenerlkaen
     1021 raltrvvskl tnttk
//
LOCUS       XP_034190411            1009 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034190411
VERSION     XP_034190411.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334520.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1009
                     /product="myosin binding subunit isoform X4"
                     /calculated_mol_wt=112080
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          631..675
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          910..1005
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..1009
                     /gene="Mbs"
                     /coded_by="XM_034334520.2:392..3421"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vaiciscnna awteatretd meesddeges etssdshsst ysnqsdksne snhspcltdd
      421 ekknranree tsprnlsspi evnkvpnqap vmppkqqtds neegvipswr rsgsfrnriq
      481 ntettntvst rleekdknii sptipnklnt etdvvltrth sfetdekfye qylalrarik
      541 afscptlhcc naatpthtnt ttrsaslret yrrkeaklnl elsrlpqgsn tlsptstskt
      601 ivsstlpttt atatttattt tttspiptnq irrsfvppvr deesetqrka hakrvretrr
      661 stqgvtldei ksaeqlvkkk qqnneipttv sstqpattts ntasitatit tatpttvttn
      721 kspeelnlpe rrpswrlkvd ngskfqleda nnrtatvpnp dtttaymrrp stgtsiprps
      781 sapvetiats saettvtlpl rrplkqpedk eqdkendsrn aqatqaviqr rrrpkrrstg
      841 vvhvdmdeid pekqdltagg dcdenkinhe sgndrsgrsn rlgsisslss etpsvsarik
      901 sttsengeld ykklyeesqa enerlkeklr rsddqlkevr nlldkaqsaq nktvlseaek
      961 rerramerkl semeeelkql qklkaenerl kaenraltrv vskltnttk
//
LOCUS       XP_034190421            1002 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034190421
VERSION     XP_034190421.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334530.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1002
                     /product="myosin binding subunit isoform X5"
                     /calculated_mol_wt=110735
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          626..668
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          903..998
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..1002
                     /gene="Mbs"
                     /coded_by="XM_034334530.2:393..3401"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vaiciscnna awteatretd meesddeges etssdshsst ysnqsdksne snhspcltdd
      421 ekknranree tsprnlsspi evnkvpnqap vmppkqqtds neegvipswr rsgsfrnriq
      481 ntettntvst rleekdknii sptipnklnt etdvvltrth sfetdekrke aklnlelsrl
      541 pqgsntlspt stsktivsst lptttatatt tattttttsp iptnqirsvq pvegtttnns
      601 vavpgtpttp ggsklspgni fknffksfvp pvrdeesetq rkahakrvre trrstqgvtl
      661 deiksaeqlv kkkqqnneip ttvsstqpat ttsntasita tittatpttv ttnkspeeln
      721 lperrpswrl kvdngskfql edannrtatv pnpdtttaym rrpstgtsip rpssapveti
      781 atssaettvt lplrrplkqp edkeqdkend srnaqatqav iqrrrrpkrr stgvvhvdmd
      841 eidpekqdlt aggdcdenki nhesgndrsg rsnrlgsiss lssetpsvsa riksttseng
      901 eldykklyee sqaenerlke klrrsddqlk evrnlldkaq saqnktvlse aekrerrame
      961 rklsemeeel kqlqklkaen erlkaenral trvvskltnt tk
//
LOCUS       XP_034190431             997 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034190431
VERSION     XP_034190431.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334540.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..997
                     /product="myosin binding subunit isoform X6"
                     /calculated_mol_wt=110544
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          647..>979
                     /region_name="Mitofilin"
                     /note="Mitochondrial inner membrane protein; pfam09731"
                     /db_xref="CDD:430783"
     Region          671..713
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          898..993
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..997
                     /gene="Mbs"
                     /coded_by="XM_034334540.2:395..3388"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vaiciscnna awteatretd meesddeges etssdshsst ysnqsdksne snhspcltdd
      421 ekknranree tsprnlsspi evnkvpnqap vmppkqqtds neegvipswr rsgsfrnriq
      481 ntettntvst rleekdknii sptipnklnt etdvvltrth sfetdekfye qylalrarik
      541 afscptlhcc naatpthtnt ttrsaslret yrrkeaklnl elsrlpqgsn tlsptstskt
      601 ivsstlpttt atatttattt tttspiptnq irsvqpvegt ttnnsvavpg tpttpggskl
      661 spgnifknff ksfvppvrde esetqrkaha krvretrrst qgvtldeiks aeqlvkkkqq
      721 nneipttvss tqfqledann rtatvpnpdt ttaymrrpst gtsiprpssa pvetiatssa
      781 ettvtlplrr plkqpedkeq dkendsrnaq atqaviqrrr rpkrrstgvv hvdmdeidpe
      841 kqdltaggdc denkinhesg ndrsgrsnrl gsisslsset psvsarikst tsengeldyk
      901 klyeesqaen erlkeklrrs ddqlkevrnl ldkaqsaqnk tvlseaekre rramerklse
      961 meeelkqlqk lkaenerlka enraltrvvs kltnttk
//
LOCUS       XP_034190441             988 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034190441
VERSION     XP_034190441.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334550.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..988
                     /product="myosin binding subunit isoform X7"
                     /calculated_mol_wt=109480
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          612..654
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          889..984
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..988
                     /gene="Mbs"
                     /coded_by="XM_034334550.2:392..3358"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vekknranre etsprnlssp ievnkvpnqa pvmppkqqtd sneegvipsw rrsgsfrnri
      421 qntettntvs trleekdkni isptipnkln tetdvvltrt hsfetdekfy eqylalrari
      481 kafscptlhc cnaatpthtn tttrsaslre tyrrkeakln lelsrlpqgs ntlsptstsk
      541 tivsstlptt tatatttatt ttttspiptn qirsvqpveg tttnnsvavp gtpttpggsk
      601 lspgnifknf fksfvppvrd eesetqrkah akrvretrrs tqgvtldeik saeqlvkkkq
      661 qnneipttvs stqpatttsn tasitatitt atpttvttnk speelnlper rpswrlkvdn
      721 gskfqledan nrtatvpnpd tttaymrrps tgtsiprpss apvetiatss aettvtlplr
      781 rplkqpedke qdkendsrna qatqaviqrr rrpkrrstgv vhvdmdeidp ekqdltaggd
      841 cdenkinhes gndrsgrsnr lgsisslsse tpsvsariks ttsengeldy kklyeesqae
      901 nerlkeklrr sddqlkevrn lldkaqsaqn ktvlseaekr erramerkls emeeelkqlq
      961 klkaenerlk aenraltrvv skltnttk
//
LOCUS       XP_034190451             967 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034190451
VERSION     XP_034190451.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334560.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..967
                     /product="myosin binding subunit isoform X8"
                     /calculated_mol_wt=107092
     Region          <1..225
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1..27
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(29,31,35..36,39..41,43..44,48,51,60,122,124,
                     128..129,132..134,136..137,141,144,153,155,157,161..162,
                     165..167,169..170,174,177,186)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          29..60
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          122..153
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          155..186
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          591..633
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          868..963
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..967
                     /gene="Mbs"
                     /coded_by="XM_034334560.2:164..3067"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mylacinddl dmveflvekg adincgdneg wtplhatasc gfisiakyli ekgcnlaavn
       61 ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers mlndarawqs gaagkdtihp
      121 ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl haaahwgqle acellvenfc
      181 nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq iinkkqptip kkrvstsnen
      241 aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd vaiciscnna awteatretd
      301 meesddeges etssdshsst ysnqsdksne snhspcltdd ekknranree tsprnlsspi
      361 evnkvpnqap vmppkqqtds neegvipswr rsgsfrnriq ntettntvst rleekdknii
      421 sptipnklnt etdvvltrth sfetdekfye qylalrarik afscptlhcc naatpthtnt
      481 ttrsaslret yrrkeaklnl elsrlpqgsn tlsptstskt ivsstlpttt atatttattt
      541 tttspiptnq irsvqpvegt ttnnsvavpg tpttpggskl spgnifknff ksfvppvrde
      601 esetqrkaha krvretrrst qgvtldeiks aeqlvkkkqq nneipttvss tqpatttsnt
      661 asitatitta tpttvttnks peelnlperr pswrlkvdng skfqledann rtatvpnpdt
      721 ttaymrrpst gtsiprpssa pvetiatssa ettvtlplrr plkqpedkeq dkendsrnaq
      781 atqaviqrrr rpkrrstgvv hvdmdeidpe kqdltaggdc denkinhesg ndrsgrsnrl
      841 gsisslsset psvsarikst tsengeldyk klyeesqaen erlkeklrrs ddqlkevrnl
      901 ldkaqsaqnk tvlseaekre rramerklse meeelkqlqk lkaenerlka enraltrvvs
      961 kltnttk
//
LOCUS       XP_034190459             903 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034190459
VERSION     XP_034190459.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334568.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..903
                     /product="myosin binding subunit isoform X9"
                     /calculated_mol_wt=100504
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          526..569
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          804..899
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..903
                     /gene="Mbs"
                     /coded_by="XM_034334568.2:392..3103"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vaiciscnna awteatretd meesddeges etssdshsst ysnqsdksne snhspcltdd
      421 ekknranree tsprnlsspi evnkvpnqap vmppkqqtds neegvipswr rsgsfrnriq
      481 ntettntvst rleekdknii sptipnklnt etdvvltrth sfetdeksfv ppvrdeeset
      541 qrkahakrvr etrrstqgvt ldeiksaeql vkkkqqnnei pttvsstqpa tttsntasit
      601 atittatptt vttnkspeel nlperrpswr lkvdngskfq ledannrtat vpnpdtttay
      661 mrrpstgtsi prpssapvet iatssaettv tlplrrplkq pedkeqdken dsrnaqatqa
      721 viqrrrrpkr rstgvvhvdm deidpekqdl taggdcdenk inhesgndrs grsnrlgsis
      781 slssetpsvs ariksttsen geldykklye esqaenerlk eklrrsddql kevrnlldka
      841 qsaqnktvls eaekrerram erklsemeee lkqlqklkae nerlkaenra ltrvvskltn
      901 ttk
//
LOCUS       XP_076546481             950 aa            linear   INV 03-SEP-2025
DEFINITION  myosin binding subunit isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_076546481
VERSION     XP_076546481.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690366.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..950
                     /product="myosin binding subunit isoform X10"
                     /calculated_mol_wt=105677
     Region          <48..305
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(53..55,57..58,62,65,74,76,78,82..83,86..88,90..91,
                     95,98,107,109,111,115..116,119..121,123..124,128,131,140)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          76..107
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          109..140
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          235..266
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          572..616
                     /region_name="IPD_PPP1R12"
                     /note="inhibitory phosphorylation domain of protein
                     phosphatase 1 regulatory subunit 12 (PPP1R12) family;
                     cd21930"
                     /db_xref="CDD:412018"
     Region          851..946
                     /region_name="PRKG1_interact"
                     /note="cGMP-dependent protein kinase interacting domain;
                     pfam15898"
                     /db_xref="CDD:464927"
     CDS             1..950
                     /gene="Mbs"
                     /coded_by="XM_076690366.1:391..3243"
                     /db_xref="GeneID:117608839"
ORIGIN      
        1 mslesrsssa lfkraeqlkr weqsetnrep aqprqvarki kfsadcvfla acaagdkeev
       61 irllqkgadi ntgnvdglta lhqacinddl dmveflvekg adincgdneg wtplhatasc
      121 gfisiakyli ekgcnlaavn ydgqlaldia qsdemedmlq qhidkagvdc dqarseeers
      181 mlndarawqs gaagkdtihp ksgatalhva aakgyvdvme illqarcdvn aqdfdgwtpl
      241 haaahwgqle acellvenfc nmdiknyadq tafdiadtsi lsalkelkak qqtlmkdhpq
      301 iinkkqptip kkrvstsnen aivmqesvet feeetpnkvk kveleiqsdk edsstgtnsd
      361 vekknranre etsprnlssp ievnkvpnqa pvmppkqqtd sneegvipsw rrsgsfrnri
      421 qntettntvs trleekdkni isptipnkln tetdvvltrt hsfetdekfy eqylalrari
      481 kafscptlhc cnaatpthtn tttrsaslre tyrrkeakln lelsrlpqgs ntlsptstsk
      541 tivsstlptt tatatttatt ttttspiptn qirrsfvppv rdeesetqrk ahakrvretr
      601 rstqgvtlde iksaeqlvkk kqqnneiptt vsstqpattt sntasitati ttatpttvtt
      661 nkspeelnlp errpswrlkv dngskfqled annrtatvpn pdtttaymrr pstgtsiprp
      721 ssapvetiat ssaettvtlp lrrplkqped keqdkendsr naqatqaviq rrrrpkrrst
      781 gvvhvdmdei dpekqdltag gdcdenkinh esgndrsgrs nrlgsissls setpsvsari
      841 ksttsengel dykklyeesq aenerlkekl rrsddqlkev rnlldkaqsa qnktvlseae
      901 krerramerk lsemeeelkq lqklkaener lkaenraltr vvskltnttk
//
LOCUS       XP_034190483             813 aa            linear   INV 03-SEP-2025
DEFINITION  LEM domain-containing inner nuclear membrane protein MAN1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190483
VERSION     XP_034190483.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334592.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..813
                     /product="LEM domain-containing inner nuclear membrane
                     protein MAN1"
                     /calculated_mol_wt=91383
     Region          3..41
                     /region_name="LEM"
                     /note="LEM domain; pfam03020"
                     /db_xref="CDD:460781"
     Region          <436..654
                     /region_name="MSC"
                     /note="Man1-Src1p-C-terminal domain; pfam09402"
                     /db_xref="CDD:430586"
     Region          699..790
                     /region_name="RRM_Man1"
                     /note="RNA recognition motif (RRM) found in inner nuclear
                     membrane protein Man1 (Man1) and similar proteins;
                     cd12286"
                     /db_xref="CDD:409728"
     CDS             1..813
                     /gene="MAN1"
                     /coded_by="XM_034334592.2:344..2785"
                     /db_xref="GeneID:117608868"
ORIGIN      
        1 msvdtlsdse lrrklmeygy pvgpvtqttr kilakklknl ietrggtgsr hslaarysse
       61 dtdddssstv skkkkttass rrqtmanpmp ppspvltssd vnilknvkdk gdsefkdpvv
      121 pdydssssft trtltktthk kymksnksss vtdgletgsd sdvieepiks yspskylsnv
      181 gkshdsmiyk hnvsdqeqip ksydaksrsi htikdnkyne svfdsnmspi qattedvcmk
      241 dnsnqrdlla syetpflsef trrlssrssv nlpstslsnl kspsscltsn vpdlkekdsn
      301 ghfsslrsly sssnagsrht tssvdrpret msrtfksspt nredtrnnhn mvsvilvmvl
      361 alffgilavi ymglsgktet fpslatdsni plcfleddle tpgvncvlke nvesvlqllk
      421 rlqpiltkka vsmicdnsse tpyltdseim qmftnsrvts levkedlqna qllviknpqw
      481 gislidisdd ngnpgdvlds ldklfstrln gkvgmmitnp elpmkclikn klftifssll
      541 ivslgllaai giqklfvwyi rykksterev fklvsdiinm vemhhqnaav atpggtqesf
      601 lainhvrdnl ippkdrrkma glweravkfl denesrirre vqqvageefh vwrwlpnntl
      661 nksntqifgl nkkskvwqgq afetmegsvn sltcsptpcl kirhmfdadv efeddwetkv
      721 qdailekcgd gvkilhirvd rgsregcvym kcmsqedagk ayralhgcwf dghlvtvkyl
      781 rleryherfp darrcttplk psnnqrlsmq vdy
//
LOCUS       XP_034190512             521 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: PI4KA lipid kinase complex subunit hyccin
            [Osmia lignaria lignaria].
ACCESSION   XP_034190512
VERSION     XP_034190512.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334621.2
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190512.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts          :: corrected 3 indels
            internal stop codons :: corrected 5 genomic stop codons
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..521
                     /product="LOW QUALITY PROTEIN: PI4KA lipid kinase complex
                     subunit hyccin"
                     /calculated_mol_wt=57619
     Region          19..343
                     /region_name="Hyccin"
                     /note="pfam09790"
                     /db_xref="CDD:462903"
     CDS             1..521
                     /gene="Hyccin"
                     /coded_by="XM_034334621.2:144..1709"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 5 bases in 3 codons;
                     substituted 5 bases at 5 genomic stop codons"
                     /db_xref="GeneID:117608884"
ORIGIN      
        1 mteslinewl tdcadvssse lhtfattlsq dneivralyv lleerskysq lvdtvcdqly
       61 nfyrsretel qrftlqflpt liyiylnsaa hgdikncrsv etlliglynl eimdksgqpk
      121 aisfrlpsla mlsvyhepas lapasltesa vrrfeecntk lvswgplqqv etltaqnrlk
      181 vmtallfiyn qqlgyiskfa leqlckvatk lvtqgfmkpg hhqrssygse ssfvprllpr
      241 ipvssqflle flhavyfamy ndcwyigtqa vedihnracy etypdvmlvt nairnsassg
      301 psgqptdgpi gisvalspat atatvsksmi tnasfrakkl pddipiqetk edsggeaksn
      361 lvsiteeest eknrvgsirq skdqktvsti tnflvrgkrn ieegrxnvik nvridppeee
      421 nefgsqttlk esiknsmlia ihxxkcxxvf nqkkkkkkmn nvvennsirv ytnislidge
      481 rlalsgdvdn tnmeapitls idnetesltl siqvgsvrtn l
//
LOCUS       XP_034190495             514 aa            linear   INV 03-SEP-2025
DEFINITION  glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial [Osmia
            lignaria lignaria].
ACCESSION   XP_034190495
VERSION     XP_034190495.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334604.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="glutamyl-tRNA(Gln) amidotransferase subunit B,
                     mitochondrial"
                     /calculated_mol_wt=58713
     Region          33..513
                     /region_name="gatB"
                     /note="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB; PRK05477"
                     /db_xref="CDD:235489"
     CDS             1..514
                     /gene="GatB"
                     /coded_by="XM_034334604.2:116..1660"
                     /db_xref="GeneID:117608875"
ORIGIN      
        1 mfvcsknyis vgkhykdvyk kfhlrftstd ftqwkstvgl eihaqiatks klfsgastnf
       61 sspinscvsl fdcaipgtmp vlnkkcveag vstalalsce vnnvsmferk hyfysdlpag
      121 fqitqqkkpl aingelkfiv ftpemqkqpy fktskikqiq leqdsgrsfh sehfernlvd
      181 lnragiplme lvfepdltng eeaaalvrel sfilqligtc sckmeegalr vdanvsvsrd
      241 naslgtrtel knigstrald naieyeikrq isvlknggki vnetrgwdsq qqktipmrek
      301 eekhdyrfmp epnlpplylh nlidveflre qlpelpeqmr qktfqdlkpq afvgitsnle
      361 lfklfrnile ngkyrnpqlv atiliseitt flqvnnlqlt ycsdnlnrig eaidliqdna
      421 inymilqkll nklviepdkt pkqiieennw fmisdeikle klcleilekq pklvkkykkg
      481 rtrvfkvllq qvasatndca nmeqttkimt rlls
//
LOCUS       XP_034190535             424 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608899 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190535
VERSION     XP_034190535.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334644.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190535.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..424
                     /product="uncharacterized protein LOC117608899 isoform X1"
                     /calculated_mol_wt=49941
     Region          1..133
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <123..401
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..424
                     /gene="LOC117608899"
                     /coded_by="XM_034334644.2:99..1373"
                     /db_xref="GeneID:117608899"
ORIGIN      
        1 mdleiekihn ilieanlpss ikdlknptee fvvdlintfl rrfhiyvgti nnplmeqqdi
       61 meyiedsdii glinlhtamv qicdriyfkd lcitdisspg skkirkfakf lanfilfann
      121 kesdieeqin eiqnrakllh dmiekknevi qtknekalyi gkqlslkeky iseiqklqsk
      181 rernnkkdae itaketaakr kkqqavelcg tykaeasklh kkvtqlqsei iespdkyemh
      241 lkelekqqss kvqerktmee acqdkkhlme qqtnifnfvq kqleklvevq diyqqlkkvd
      301 vqednikkqv dmlrtdianf ekrlenqkgq reetevhdiq aqyeerlspl rnlssqllsd
      361 kklsevklee mkeqynegyl kldemrsttq kledettvli knyqdlydne iltekalwet
      421 wsne
//
LOCUS       XP_034190546             418 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117608899 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190546
VERSION     XP_034190546.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334655.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190546.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..418
                     /product="uncharacterized protein LOC117608899 isoform X2"
                     /calculated_mol_wt=49343
     Region          1..127
                     /region_name="Nuf2"
                     /note="Nuf2 family; pfam03800"
                     /db_xref="CDD:461057"
     Region          <117..395
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..418
                     /gene="LOC117608899"
                     /coded_by="XM_034334655.2:100..1356"
                     /db_xref="GeneID:117608899"
ORIGIN      
        1 mdleiekihn ilieanlpss ikdlknptee fvvdlintfl rrfhiyplme qqdimeyied
       61 sdiiglinlh tamvqicdri yfkdlcitdi sspgskkirk fakflanfil fannkesdie
      121 eqineiqnra kllhdmiekk neviqtknek alyigkqlsl kekyiseiqk lqskrernnk
      181 kdaeitaket aakrkkqqav elcgtykaea sklhkkvtql qseiiespdk yemhlkelek
      241 qqsskvqerk tmeeacqdkk hlmeqqtnif nfvqkqlekl vevqdiyqql kkvdvqedni
      301 kkqvdmlrtd ianfekrlen qkgqreetev hdiqaqyeer lsplrnlssq llsdkklsev
      361 kleemkeqyn egylkldemr sttqkledet tvliknyqdl ydneilteka lwetwsne
//
LOCUS       XP_034190228             907 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190228
VERSION     XP_034190228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334337.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..907
                     /product="glucose transporter 1 isoform X1"
                     /calculated_mol_wt=100012
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..907
                     /gene="Glut1"
                     /coded_by="XM_034334337.2:880..3603"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgnds rsslrdsrly
      841 gsmlncvnal eghippaesa almvaeekph pdsfvfaags erssgapaqp erpppplppp
      901 rfpnsgv
//
LOCUS       XP_034190236             907 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190236
VERSION     XP_034190236.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334345.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..907
                     /product="glucose transporter 1 isoform X1"
                     /calculated_mol_wt=100012
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..907
                     /gene="Glut1"
                     /coded_by="XM_034334345.2:1071..3794"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgnds rsslrdsrly
      841 gsmlncvnal eghippaesa almvaeekph pdsfvfaags erssgapaqp erpppplppp
      901 rfpnsgv
//
LOCUS       XP_034190245             906 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190245
VERSION     XP_034190245.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334354.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..906
                     /product="glucose transporter 1 isoform X2"
                     /calculated_mol_wt=99925
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..906
                     /gene="Glut1"
                     /coded_by="XM_034334354.2:878..3598"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgndr sslrdsrlyg
      841 smlncvnale ghippaesaa lmvaeekphp dsfvfaagse rssgapaqpe rpppplpppr
      901 fpnsgv
//
LOCUS       XP_034190253             900 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190253
VERSION     XP_034190253.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334362.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..900
                     /product="glucose transporter 1 isoform X3"
                     /calculated_mol_wt=99141
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..808
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     729..730,734,738,761,765)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..900
                     /gene="Glut1"
                     /coded_by="XM_034334362.2:883..3585"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikem idwmsylsvv
      721 stlsfvvffa vgpgsipwmi taelfsqgpr paamsiavlv nwmanflvgi gfpsmktsle
      781 nytflpfsaf laifwiftyk kvpetknktf eeilalfrhg ndsrsslrds rlygsmlncv
      841 naleghippa esaalmvaee kphpdsfvfa agserssgap aqperppppl ppprfpnsgv
//
LOCUS       XP_034190268             882 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034190268
VERSION     XP_034190268.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334377.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..882
                     /product="glucose transporter 1 isoform X4"
                     /calculated_mol_wt=97590
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..882
                     /gene="Glut1"
                     /coded_by="XM_034334377.2:876..3524"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgnds rsslrdsrly
      841 gcvcswirai frrtrstget tapaspatls eqrcltmgns ss
//
LOCUS       XP_034190274             881 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034190274
VERSION     XP_034190274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334383.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="glucose transporter 1 isoform X5"
                     /calculated_mol_wt=97503
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..881
                     /gene="Glut1"
                     /coded_by="XM_034334383.2:875..3520"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgndr sslrdsrlyg
      841 cvcswiraif rrtrstgett apaspatlse qrcltmgnss s
//
LOCUS       XP_034190298             870 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034190298
VERSION     XP_034190298.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334407.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..870
                     /product="glucose transporter 1 isoform X6"
                     /calculated_mol_wt=96254
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..870
                     /gene="Glut1"
                     /coded_by="XM_034334407.2:875..3487"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgnds rsslrdsrly
      841 geeelatnss ketvlsdatt anhthatdkp
//
LOCUS       XP_034190306             869 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034190306
VERSION     XP_034190306.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334415.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="glucose transporter 1 isoform X7"
                     /calculated_mol_wt=96167
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..869
                     /gene="Glut1"
                     /coded_by="XM_034334415.2:872..3481"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgndr sslrdsrlyg
      841 eeelatnssk etvlsdatta nhthatdkp
//
LOCUS       XP_034190316             859 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034190316
VERSION     XP_034190316.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334425.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..859
                     /product="glucose transporter 1 isoform X8"
                     /calculated_mol_wt=95032
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..815
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     736..737,741,745,768,772)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..859
                     /gene="Glut1"
                     /coded_by="XM_034334425.2:871..3450"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikef fgyvqemidw
      721 msylsvvstl sfvvffavgp gsipwmitae lfsqgprpaa msiavlvnwm anflvgigfp
      781 smktslenyt flpfsaflai fwiftykkvp etknktfeei lalfrhgndr eeelatnssk
      841 etvlsdatta nhthatdkp
//
LOCUS       XP_034190333             833 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034190333
VERSION     XP_034190333.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334442.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..833
                     /product="glucose transporter 1 isoform X9"
                     /calculated_mol_wt=92216
     Region          230..>342
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          362..808
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(374,378,509,512..513,516,630..631,635..636,639,667,
                     729..730,734,738,761,765)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..833
                     /gene="Glut1"
                     /coded_by="XM_034334442.2:873..3374"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 madsrgfkpi nqlwqsaavs tdpdseclye eisglsgsqd tepgpagaav geeeeeeeee
       61 eeeeeeeeee eeeeeeeeer garrldfgaq vggsswwrhh nkggsernvg naagtgsgrk
      121 ssksvgtsag srtrsadrps sgrrniirve eeqyasrrsy vesgtgailp ppatghkltr
      181 gratntvary lhsastntgh yhhhhhglhg qypagstgrh hqhhhpsrgs fsnetqeeiq
      241 eddeatlrel lvslqkqvsv msmnlsakld elqrgdrqle ttvalceirt qlqeltksve
      301 scqsevsevk rdmvaikhel dtvqqvkeei eelreyvdrl eehshrrklr lleqgltlfl
      361 syailaavlg mlqfgyntgv inapevnien fmkdvykdry gedisddsvk klysvavsif
      421 aiggmlggfs ggiianrfgr kgglllnnvl givgaclmgc tkiahsyeml ffgrfiigvn
      481 cglntslvpm yiseiaplnl rgglgtvnql avtvgllvsq vlgieqilgt negwpvllgl
      541 avcpailqll llpvcpespr yllitkqwee earkalrrlr asnqveedie emraeeraqq
      601 aesrismtel icsptlrapl vigvvmqlsq qlsginavfy ystnlftssg lteesakfat
      661 igigaimvcm tlvsiplmdr tgrrtlhlyg lggmfifsif itisflikem idwmsylsvv
      721 stlsfvvffa vgpgsipwmi taelfsqgpr paamsiavlv nwmanflvgi gfpsmktsle
      781 nytflpfsaf laifwiftyk kvpetknktf eeilalfrhg ndrsslrdsr lyg
//
LOCUS       XP_034190340             661 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_034190340
VERSION     XP_034190340.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334449.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="glucose transporter 1 isoform X10"
                     /calculated_mol_wt=72951
     Region          7..>96
                     /region_name="COG4372"
                     /note="Uncharacterized protein, contains DUF3084 domain
                     [Function unknown]"
                     /db_xref="CDD:443500"
     Region          116..569
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(128,132,263,266..267,270,384..385,389..390,393,421,
                     490..491,495,499,522,526)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..661
                     /gene="Glut1"
                     /coded_by="XM_034334449.2:280..2265"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 mvslppslqk qvsvmsmnls akldelqrgd rqlettvalc eirtqlqelt ksvescqsev
       61 sevkrdmvai kheldtvqqv keeieelrey vdrleehshr rklrlleqgl tlflsyaila
      121 avlgmlqfgy ntgvinapev nienfmkdvy kdrygedisd dsvkklysva vsifaiggml
      181 ggfsggiian rfgrkgglll nnvlgivgac lmgctkiahs yemlffgrfi igvncglnts
      241 lvpmyiseia plnlrgglgt vnqlavtvgl lvsqvlgieq ilgtnegwpv llglavcpai
      301 lqllllpvcp espryllitk qweeearkal rrlrasnqve edieemraee raqqaesris
      361 mtelicsptl raplvigvvm qlsqqlsgin avfyystnlf tssglteesa kfatigigai
      421 mvcmtlvsip lmdrtgrrtl hlyglggmfi fsifitisfl ikeffgyvqe midwmsylsv
      481 vstlsfvvff avgpgsipwm itaelfsqgp rpaamsiavl vnwmanflvg igfpsmktsl
      541 enytflpfsa flaifwifty kkvpetknkt feeilalfrh gndsrsslrd srlygsmlnc
      601 vnaleghipp aesaalmvae ekphpdsfvf aagserssga paqperpppp lppprfpnsg
      661 v
//
LOCUS       XP_034190358             560 aa            linear   INV 03-SEP-2025
DEFINITION  glucose transporter 1 isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_034190358
VERSION     XP_034190358.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334467.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..560
                     /product="glucose transporter 1 isoform X11"
                     /calculated_mol_wt=61135
     Region          15..468
                     /region_name="MFS_GLUT_Class1"
                     /note="Class 1 Glucose transporters (GLUTs) of the Major
                     Facilitator Superfamily; cd17431"
                     /db_xref="CDD:340989"
     Site            order(27,31,162,165..166,169,283..284,288..289,292,320,
                     389..390,394,398,421,425)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:340989"
     CDS             1..560
                     /gene="Glut1"
                     /coded_by="XM_034334467.2:803..2485"
                     /db_xref="GeneID:117608759"
ORIGIN      
        1 macesfkglt lflsyailaa vlgmlqfgyn tgvinapevn ienfmkdvyk drygedisdd
       61 svkklysvav sifaiggmlg gfsggiianr fgrkggllln nvlgivgacl mgctkiahsy
      121 emlffgrfii gvncglntsl vpmyiseiap lnlrgglgtv nqlavtvgll vsqvlgieqi
      181 lgtnegwpvl lglavcpail qllllpvcpe spryllitkq weeearkalr rlrasnqvee
      241 dieemraeer aqqaesrism telicsptlr aplvigvvmq lsqqlsgina vfyystnlft
      301 ssglteesak fatigigaim vcmtlvsipl mdrtgrrtlh lyglggmfif sifitisfli
      361 keffgyvqem idwmsylsvv stlsfvvffa vgpgsipwmi taelfsqgpr paamsiavlv
      421 nwmanflvgi gfpsmktsle nytflpfsaf laifwiftyk kvpetknktf eeilalfrhg
      481 ndsrsslrds rlygsmlncv naleghippa esaalmvaee kphpdsfvfa agserssgap
      541 aqperppppl ppprfpnsgv
//
LOCUS       XP_034190368             292 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit 27 [Osmia lignaria lignaria].
ACCESSION   XP_034190368
VERSION     XP_034190368.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334477.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="mediator complex subunit 27"
                     /calculated_mol_wt=32997
     Region          209..290
                     /region_name="Med27"
                     /note="Mediator complex subunit 27; pfam11571"
                     /db_xref="CDD:463298"
     CDS             1..292
                     /gene="MED27"
                     /coded_by="XM_034334477.2:339..1217"
                     /db_xref="GeneID:117608832"
ORIGIN      
        1 meqlqtalta ikvlrssvgq vfdslgnglr adhgeenken kyllelqell ttvnvnlrdv
       61 eqavsslnpp pgpfnlgsta ylsqettqer qalygtlvns ykwtdkvhey snvaqmllsq
      121 nslkrsyntp srakrgriqt snhnvpqqqv dsliatfdrl fndmtvsvsr pfasnavlhv
      181 tlghvlkgvi afkglmiewv vvkgygetmd lwtesrhkvf rkvtenahaa mlhfyspalp
      241 elavrsfmtw fhslnnlfsd pckrcglhlh salpptwrdf rtlepyhqec kp
//
LOCUS       XP_034190557             893 aa            linear   INV 03-SEP-2025
DEFINITION  staphylococcal nuclease domain-containing protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190557
VERSION     XP_034190557.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334666.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..893
                     /product="staphylococcal nuclease domain-containing
                     protein 1"
                     /calculated_mol_wt=100805
     Region          9..143
                     /region_name="SNc"
                     /note="Staphylococcal nuclease homologues. SNase
                     homologues are found in bacteria, archaea, and eukaryotes.
                     They contain no disufide bonds; cl00140"
                     /db_xref="CDD:469627"
     Site            order(22,40,46,49,93..94,96)
                     /site_type="other"
                     /note="Catalytic site"
                     /db_xref="CDD:238102"
     Region          182..317
                     /region_name="SNc"
                     /note="Staphylococcal nuclease homologues; smart00318"
                     /db_xref="CDD:214615"
     Site            order(195,210,216,259..260,261)
                     /site_type="other"
                     /note="Catalytic site"
                     /db_xref="CDD:238102"
     Region          334..478
                     /region_name="SNc"
                     /note="Staphylococcal nuclease homologues. SNase
                     homologues are found in bacteria, archaea, and eukaryotes.
                     They contain no disufide bonds; cl00140"
                     /db_xref="CDD:469627"
     Site            order(338,352,358,369,417..418,420)
                     /site_type="other"
                     /note="Catalytic site"
                     /db_xref="CDD:238102"
     Region          509..644
                     /region_name="SNc"
                     /note="Staphylococcal nuclease homologues. SNase
                     homologues are found in bacteria, archaea, and eukaryotes.
                     They contain no disufide bonds; cl00140"
                     /db_xref="CDD:469627"
     Site            order(520,535,541,544,586..587,590)
                     /site_type="other"
                     /note="Catalytic site"
                     /db_xref="CDD:238102"
     Region          693..771
                     /region_name="Tudor_TDRD11"
                     /note="Tudor domain found in Tudor domain-containing
                     protein 11 (TDRD11) and similar proteins; cd20433"
                     /db_xref="CDD:410504"
     Site            order(694,726,729,733,749,752,754)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:410504"
     Region          <835..880
                     /region_name="SNc"
                     /note="Staphylococcal nuclease homologues. SNase
                     homologues are found in bacteria, archaea, and eukaryotes.
                     They contain no disufide bonds; cl00140"
                     /db_xref="CDD:469627"
     CDS             1..893
                     /gene="Tudor-SN"
                     /coded_by="XM_034334666.2:267..2948"
                     /db_xref="GeneID:117608907"
ORIGIN      
        1 msapqaqvks rngvvkqvas gdtivirgqp mggpppeati tlcnitapkl erwkgndstd
       61 eskdepyawe areflrkkli gqdvtfvtek sintnrtygt vwlgkdksge niietlvseg
      121 lvtvkkdtrn pspeqtrlie lenaakaakk gkwsesplse hvrdvkwtvd dprklvekfg
      181 kkpvkaiiey vfdgstvkal llpdfynvvl misgvrcpgw pngrreysvg dpyadearyf
      241 vesrllhrdv eivlesvnnn nfigsilhpk gniaeillse gfakcqdwsi nnsragaekl
      301 ylaekaakea rlrlwkdykp sgpqieftgt vveivnadal mirtqngenk kvflssirpp
      361 srekkandet nnanrkdfkp lydipwmlea reflrekfir knvkvvvdyt qpardnfpek
      421 lcctvtcgkt niaealvarg lvrvikyrqn ddqrsshynl lqvaeskaek sqhglhakkd
      481 vpvhrlvdls ndppkakafl tslkraqgik avvefvtsgs rlklflpked qlitfvlagi
      541 rtprcqrslp sggvvkadey gekalaftre hcfqrdveik iestetkgsg figwltvndi
      601 nmsvslveeg laevvtfpdf geltrtlkaa eeraktkkln mwknfvevqv enekneneke
      661 iverkidyqe vvisevtddl hfyaqsvdqr smletlllql rqelasnppl pgaykatrgd
      721 lavakftgdd qwyrvkiekv sgtnvsvfyi dygnretvnv trvadlpsrf atdkpyaheh
      781 vpacvtlpgd nddkklaves fkedvmdkil llnteyklss svtavtlvds stnddivkgl
      841 isdglllvqn qrdrrltkli eeyrkaeeda khsrrniwry gdirtddeke fgl
//
LOCUS       XP_034190569             729 aa            linear   INV 03-SEP-2025
DEFINITION  sodium-dependent neutral amino acid transporter B(0)AT3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190569
VERSION     XP_034190569.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334678.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..729
                     /product="sodium-dependent neutral amino acid transporter
                     B(0)AT3"
                     /calculated_mol_wt=80556
     Region          92..665
                     /region_name="SLC6sbd-B0AT-like"
                     /note="System B(0) neutral amino acid transporter AT1, 2
                     and 3, and related proteins; solute-binding domain;
                     cd10332"
                     /db_xref="CDD:271364"
     Site            order(107,110,492,495..496)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:271364"
     Site            order(108..109,111..114,189,340..341,346,348,496,499..500)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:271364"
     Site            order(109,114,341,373)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:271364"
     Site            order(116..117,188,192,195,461,465)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:271364"
     CDS             1..729
                     /gene="LOC117608916"
                     /coded_by="XM_034334678.2:1..2190"
                     /db_xref="GeneID:117608916"
ORIGIN      
        1 mantahlvrr qssrdlkpqk svdklemkem rgrlvvdnrk nittanygat naafedsspn
       61 tknktgnggg vsklgsnegk aifrpeaged erenwdsklt fllatvgyav glgnvwrfpy
      121 laqkngggaf lipyfvmlai egipifylel aigqrlrkga igvwnqvspy mggigissav
      181 vsfnvalyyn tiiawclfyf vqsfqsqlpw aecpniyfqn gsyapepecv vssptqyfwy
      241 rttlmisddi ntpelfnwki alalviawil vymcmikgia ssgkvvyvta tfpyivliif
      301 ffrgvtlkgm sdglrhlftp kwwkltdpvv wleagtqiff slglafggli afssynpvnn
      361 ncyrdaimvs ltncftsmfa givvfsiigf katlvyercl sernstlmni fgqidkipdd
      421 ipisgtllni ttgngtldnl impelpecdl qkeldnsasg tglafiifte ainqfpgaqf
      481 wsvlfflmlf tlgidsqfgt legvvtsivd mklfpnlrke iltggiclvc cvismafahg
      541 agsyvfvlfd nfsgnfplli iaffeciavs yvyglkrfad dielmtgnrp glywlicwky
      601 lsplamlsil vasvveilvd gsgypawvas kgitekhewp mwsigligll ilasvlwipa
      661 vaicrlfgil iiednekawf paadlkefhg ivphevtpae tllfcirsdg seglccptgg
      721 psdddedlt
//
LOCUS       XP_034190587             337 aa            linear   INV 03-SEP-2025
DEFINITION  methylosome protein WDR77 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190587
VERSION     XP_034190587.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334696.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="methylosome protein WDR77 isoform X1"
                     /calculated_mol_wt=37422
     Region          <126..326
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          130..166
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          174..210
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          216..251
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          261..301
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..337
                     /gene="LOC117608929"
                     /coded_by="XM_034334696.2:116..1129"
                     /db_xref="GeneID:117608929"
ORIGIN      
        1 msslidpnln advyrnmtaa drppvpdkhl qfiliyneks ailggsnmtd rywsgtvwyy
       61 ndisdfnrdk gfvatktesg vcdaiyleed kfvitedsgv lqifslaeta etnlqelqcm
      121 gysclhdsst lsisafsdkd hvvtggmdcc lkvwdlsell vthsfgyahi dvvtcvdaqp
      181 ksttafvsts sdcealmwdi rqskpaqsil kkdigltavn wnpnsdnila igtdggdvvl
      241 idirktdtsv leetcifprs vhkllfnpns erseelaccc ddnivkvldt nsqlssiykd
      301 eshsdfvrgl twfkdalysc swdntvlkht ihsvqnv
//
LOCUS       XP_034190595             290 aa            linear   INV 03-SEP-2025
DEFINITION  methylosome protein WDR77 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190595
VERSION     XP_034190595.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334704.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..290
                     /product="methylosome protein WDR77 isoform X2"
                     /calculated_mol_wt=32239
     Region          <79..279
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          83..119
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          127..163
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          169..204
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          214..254
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..290
                     /gene="LOC117608929"
                     /coded_by="XM_034334704.2:142..1014"
                     /db_xref="GeneID:117608929"
ORIGIN      
        1 mtdrywsgtv wyyndisdfn rdkgfvatkt esgvcdaiyl eedkfvited sgvlqifsla
       61 etaetnlqel qcmgysclhd sstlsisafs dkdhvvtggm dcclkvwdls ellvthsfgy
      121 ahidvvtcvd aqpksttafv stssdcealm wdirqskpaq silkkdiglt avnwnpnsdn
      181 ilaigtdggd vvlidirktd tsvleetcif prsvhkllfn pnserseela cccddnivkv
      241 ldtnsqlssi ykdeshsdfv rgltwfkdal yscswdntvl khtihsvqnv
//
LOCUS       XP_076546483             290 aa            linear   INV 03-SEP-2025
DEFINITION  methylosome protein WDR77 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546483
VERSION     XP_076546483.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690368.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..290
                     /product="methylosome protein WDR77 isoform X2"
                     /calculated_mol_wt=32239
     Region          <79..279
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          83..119
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          127..163
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          169..204
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          214..254
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..290
                     /gene="LOC117608929"
                     /coded_by="XM_076690368.1:177..1049"
                     /db_xref="GeneID:117608929"
ORIGIN      
        1 mtdrywsgtv wyyndisdfn rdkgfvatkt esgvcdaiyl eedkfvited sgvlqifsla
       61 etaetnlqel qcmgysclhd sstlsisafs dkdhvvtggm dcclkvwdls ellvthsfgy
      121 ahidvvtcvd aqpksttafv stssdcealm wdirqskpaq silkkdiglt avnwnpnsdn
      181 ilaigtdggd vvlidirktd tsvleetcif prsvhkllfn pnserseela cccddnivkv
      241 ldtnsqlssi ykdeshsdfv rgltwfkdal yscswdntvl khtihsvqnv
//
LOCUS       XP_034190605             256 aa            linear   INV 03-SEP-2025
DEFINITION  VAMP-associated protein 33kDa [Osmia lignaria lignaria].
ACCESSION   XP_034190605
VERSION     XP_034190605.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334714.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="VAMP-associated protein 33kDa"
                     /calculated_mol_wt=28087
     Region          8..109
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     Region          10..252
                     /region_name="SCS2"
                     /note="VAMP-associated protein involved in inositol
                     metabolism [Intracellular trafficking and secretion];
                     COG5066"
                     /db_xref="CDD:227398"
     CDS             1..256
                     /gene="Vap33"
                     /coded_by="XM_034334714.2:171..941"
                     /db_xref="GeneID:117608939"
ORIGIN      
        1 mvkpeqvlii epqselrfrg pftgstvtsy ikltnpsnhk vyfkikttap krycvrpncg
       61 tvrpkevtqi avtlqpfdfd paeknkhkfm vqaliapddd dedysdvwkd tnpdqlmesk
      121 lkcvfenpvt ntttakspst atttktevnt nngknkgvgd svksspkilg eteeklikaa
      181 qevnqlrvee stlrqenlql kedllklqna algndatlia argltspsss qlspiptsvl
      241 iaivmailgy vlgkli
//
LOCUS       XP_034181752            1064 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605050 [Osmia lignaria lignaria].
ACCESSION   XP_034181752
VERSION     XP_034181752.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325861.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181752.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1064
                     /product="uncharacterized protein LOC117605050"
                     /calculated_mol_wt=117759
     Region          783..>849
                     /region_name="SEA"
                     /note="SEA domain; pfam01390"
                     /db_xref="CDD:460188"
     CDS             1..1064
                     /gene="LOC117605050"
                     /coded_by="XM_034325861.2:296..3490"
                     /db_xref="GeneID:117605050"
ORIGIN      
        1 mskcnengtw hvnvivalll fafyllptnc qqgtsdswdr fsrliptnsv yeqqdqnasp
       61 vagnpaetlp rsldennvmg gwrtnsknde reeetdeevy edeyeveesa kiehsenskl
      121 sritdgtskn dklddasrde dyvedpstsq ydayyyyddy esnnrsryra vanadtldyp
      181 daeeeekeee esspkkehvn epeereenss ekeesvdslr kviandsssd lleekdtdtp
      241 vsstnaeegs smegsdqtfa fvgspnakln ssssdssili geavvsvvtt ksvvngtisi
      301 pttsapttte qvaappssmg tteqpvttvt tedtskllas vqtsrsisga rflpfpvidr
      361 iepigekkts papestesii dkldrvqsel ssgflaggfr tagnslqldv lgdrdrgtrr
      421 rytttaktpv iskfvprryn dkrtdygngt pkpriettld slegllprdy lvkgsttstp
      481 sktgfrwptk sttsteatst tqvastafvs ttkkpksnvv vqdisaflpp gyklkkedvt
      541 tentllrdil skskvdissl lppdyskknn denssvkndv tttttttstt aksinvpsek
      601 sppvksihdl fskstfdiss llprdydqkk knqtsetknv evpkesstes nvsigtesas
      661 ttqkagglki vfpsrpggrk piykittpqs prseapgavt pkiqkgwptr atteftgwpt
      721 psttpisiek lleaartati ssvnaslvtt teatsststt stttttsrpt tpgvceqece
      781 vagtikiign atwvpelldr ntqewqnlan eiekemnfif sksavlakwy kkvridsfse
      841 gsilvdyfve ltdleqkvnt qelkvifhds lrtyntnrwn etqtkgsikl gsftidpkyt
      901 dfvvipklsg pqysekddkl ipqwtiaviv igvggllfii ifgvsvlinr hnasklkpsi
      961 stiyeeevak nitshrssdy skpvhtiwtd pdvswndksf estsnkvlve ksfqdnkkyn
     1021 mydswrsewn gyyyngshts skyggydstt nlssrhhpdy dtnf
//
LOCUS       XP_034181766             714 aa            linear   INV 03-SEP-2025
DEFINITION  protein SERAC1 [Osmia lignaria lignaria].
ACCESSION   XP_034181766
VERSION     XP_034181766.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325875.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181766.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..714
                     /product="protein SERAC1"
                     /calculated_mol_wt=82394
     Region          <519..578
                     /region_name="EstA"
                     /note="Triacylglycerol esterase/lipase EstA, alpha/beta
                     hydrolase fold [Lipid transport and metabolism]; COG1075"
                     /db_xref="CDD:440693"
     CDS             1..714
                     /gene="LOC117605056"
                     /coded_by="XM_034325875.2:191..2335"
                     /db_xref="GeneID:117605056"
ORIGIN      
        1 mgiqtyrkyi yylkstgici vmmggcwvly qlrqitqilq stlptnvlnl ehahaqyiyv
       61 ddprfkdiln kteslqssvp kqhvafsdai mkwwkavnhn mtykllhmaq hgneserlra
      121 lytlnstkhl kdwhyqhiaq mldartavsi arlpnvdlrf flkppyshmk hklhdvieri
      181 hqlllelsrl cehthpcliq flnkkfkdty sdsmildhdl tslglvvapd vvwdqellqn
      241 cvqalchhsc leeyskdiad vgglpvlmii kkffqnnini cillakiisn islhseyleq
      301 ffqagwigtl vewsqnsdir lsapasrala nldmdenkdv kyprriyllh plnkirgkik
      361 mdvvflhgll ggvfitwrqr didtstlgvv dsytipdgie tvaslmddqs peflkdlayd
      421 frkhewdkig qdfeiilddc pqnsnakssg pffhkgndic tkkyeydkqh rtqcwpkdwl
      481 pkdipslrvi ginydtnlsm wtpscpidgi qttmnersae yvkklltagi gkrpmiwvch
      541 smggllvkqm linewkngdk nnickntrgi vfystphrgs rvaalkqtvq mlvwpsvevq
      601 elreesphll elhkefldml kqypmevvsf setkstlita lkvpfqfvts nsadpgigef
      661 yeiaqdhlsi ckpasrysfl yqkllsmikh hvtpheenkf spivelvsls krlf
//
LOCUS       XP_034181784             698 aa            linear   INV 03-SEP-2025
DEFINITION  minichromosome maintenance 10 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034181784
VERSION     XP_034181784.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034181784.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..698
                     /product="minichromosome maintenance 10 homolog"
                     /calculated_mol_wt=78798
     Region          275..319
                     /region_name="zf-primase"
                     /note="Primase zinc finger; pfam09329"
                     /db_xref="CDD:462758"
     Region          361..660
                     /region_name="Mcm10"
                     /note="Mcm10 replication factor; pfam09332"
                     /db_xref="CDD:462760"
     CDS             1..698
                     /gene="Mcm10"
                     /coded_by="XM_034325893.2:314..2410"
                     /db_xref="GeneID:117605064"
ORIGIN      
        1 mdndsdsdvd dvlndllaig eentqdsspk ktlkeldfnf vdsvpktnde enesnkqdks
       61 evyddtldss ddedrryfee qkysnygrdi ksllkkegsn qnenrprais skafdfsatn
      121 kstaistttn nnntkskdvy sdpffglrii npmvssaelt srmngkipvt vskmkyhlns
      181 eniekdwvia gvvisksppk tskngssysi wkisdlsdti ntvslfmfst ayksfwkttv
      241 gtvigvlnpn ileskdnidq atlsidnpqk vmilgtskdl gkcrsrkkng dpcssivnls
      301 rcefclyhik qeykkcstrs elqaysntqk fsvdmlkkdk pkkslncnmp efnaivavks
      361 kklqekdakr lallsginkp ensastklay dmkdkilkes laqqiqknme klkesrgwra
      421 tvlsqasndt ssssptlsql nkskkqdlsm lssssrprlg lgcqggtidl sepitkkqmn
      481 vaknnaikwv qkngkikpsd pnkirlnked klekgkkrsr esendetqea kkpnvisdkf
      541 kelmqtksah tdliersyqe eeekyfnkle mkermeekml ntykvsckav kclickytsf
      601 sasdmckeqk hplrvidatk rffkctdcan rtvsldrips hscikcsssn wsraammder
      661 rtnvavtals irgdeetfmg smtkdanlnl lvpekddk
//
LOCUS       XP_034181796             437 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic release factor 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181796
VERSION     XP_034181796.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325905.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..437
                     /product="eukaryotic release factor 1 isoform X1"
                     /calculated_mol_wt=49065
     Region          13..417
                     /region_name="aRF1/eRF1"
                     /note="peptide chain release factor 1, archaeal and
                     eukaryotic forms; TIGR03676"
                     /db_xref="CDD:274719"
     CDS             1..437
                     /gene="eRF1"
                     /coded_by="XM_034325905.2:319..1632"
                     /db_xref="GeneID:117605069"
ORIGIN      
        1 msneetsadr nveiwkikkl ikslemargn gtsmisliip pkdqisrvsk mladefgtas
       61 niksrvnrls vlgaitsvqh rlklytkvpp nglviycgti vteegkekkv nidfepfkpi
      121 ntslylcdnk fhtealtall addnkfgfiv mdgngalfgt lqgntrevlh kftvdlpkkh
      181 grggqsalrf arlrmekrhn yvrkvaevat qlyitndkpn iaglilagsa dfktelsqsd
      241 mfdprlqakv iklvdvsygg engfnqaiel aaeslqnvkf iqekkligry fdeisqdtgk
      301 ycfgvedtlr alelgsvetl icwenldiqr yvlknhtnae ekvlhltpeq ekdkthftdk
      361 esgvelelve cqpllewlan nyksfgatle iitdksqegs qfvrgfggig gilrykvdfq
      421 smqledvevd sfdlddy
//
LOCUS       XP_076546485             437 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic release factor 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546485
VERSION     XP_076546485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690370.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..437
                     /product="eukaryotic release factor 1 isoform X1"
                     /calculated_mol_wt=49065
     Region          13..417
                     /region_name="aRF1/eRF1"
                     /note="peptide chain release factor 1, archaeal and
                     eukaryotic forms; TIGR03676"
                     /db_xref="CDD:274719"
     CDS             1..437
                     /gene="eRF1"
                     /coded_by="XM_076690370.1:297..1610"
                     /db_xref="GeneID:117605069"
ORIGIN      
        1 msneetsadr nveiwkikkl ikslemargn gtsmisliip pkdqisrvsk mladefgtas
       61 niksrvnrls vlgaitsvqh rlklytkvpp nglviycgti vteegkekkv nidfepfkpi
      121 ntslylcdnk fhtealtall addnkfgfiv mdgngalfgt lqgntrevlh kftvdlpkkh
      181 grggqsalrf arlrmekrhn yvrkvaevat qlyitndkpn iaglilagsa dfktelsqsd
      241 mfdprlqakv iklvdvsygg engfnqaiel aaeslqnvkf iqekkligry fdeisqdtgk
      301 ycfgvedtlr alelgsvetl icwenldiqr yvlknhtnae ekvlhltpeq ekdkthftdk
      361 esgvelelve cqpllewlan nyksfgatle iitdksqegs qfvrgfggig gilrykvdfq
      421 smqledvevd sfdlddy
//
LOCUS       XP_034181807             436 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic release factor 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181807
VERSION     XP_034181807.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325916.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..436
                     /product="eukaryotic release factor 1 isoform X2"
                     /calculated_mol_wt=48868
     Region          13..417
                     /region_name="aRF1/eRF1"
                     /note="peptide chain release factor 1, archaeal and
                     eukaryotic forms; TIGR03676"
                     /db_xref="CDD:274719"
     CDS             1..436
                     /gene="eRF1"
                     /coded_by="XM_034325916.2:321..1631"
                     /db_xref="GeneID:117605069"
ORIGIN      
        1 msneetsadr nveiwkikkl ikslemargn gtsmisliip pkdqisrvsk mladefgtas
       61 niksrvnrls vlgaitsvqh rlklytkvpp nglviycgti vteegkekkv nidfepfkpi
      121 ntslylcdnk fhtealtall addnkfgfiv mdgngalfgt lqgntrevlh kftvdlpkkh
      181 grggqsalrf arlrmekrhn yvrkvaevat qlyitndkpn iaglilagsa dfktelsqsd
      241 mfdprlqakv iklvdvsygg engfnqaiel aaeslqnvkf iqekkligry fdeisqdtgk
      301 ycfgvedtlr alelgsvetl icwenldiqr yvlknhtnae ekvlhltpeq ekdkthftdk
      361 esgvelelve cqpllewlan nyksfgatle iitdksqegs qfvrgfggig gilrykvdfq
      421 slqadepldd vdlddy
//
LOCUS       XP_034181817             436 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic release factor 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181817
VERSION     XP_034181817.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325926.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..436
                     /product="eukaryotic release factor 1 isoform X2"
                     /calculated_mol_wt=48868
     Region          13..417
                     /region_name="aRF1/eRF1"
                     /note="peptide chain release factor 1, archaeal and
                     eukaryotic forms; TIGR03676"
                     /db_xref="CDD:274719"
     CDS             1..436
                     /gene="eRF1"
                     /coded_by="XM_034325926.2:321..1631"
                     /db_xref="GeneID:117605069"
ORIGIN      
        1 msneetsadr nveiwkikkl ikslemargn gtsmisliip pkdqisrvsk mladefgtas
       61 niksrvnrls vlgaitsvqh rlklytkvpp nglviycgti vteegkekkv nidfepfkpi
      121 ntslylcdnk fhtealtall addnkfgfiv mdgngalfgt lqgntrevlh kftvdlpkkh
      181 grggqsalrf arlrmekrhn yvrkvaevat qlyitndkpn iaglilagsa dfktelsqsd
      241 mfdprlqakv iklvdvsygg engfnqaiel aaeslqnvkf iqekkligry fdeisqdtgk
      301 ycfgvedtlr alelgsvetl icwenldiqr yvlknhtnae ekvlhltpeq ekdkthftdk
      361 esgvelelve cqpllewlan nyksfgatle iitdksqegs qfvrgfggig gilrykvdfq
      421 slqadepldd vdlddy
//
LOCUS       XP_076546486             436 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic release factor 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546486
VERSION     XP_076546486.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690371.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..436
                     /product="eukaryotic release factor 1 isoform X2"
                     /calculated_mol_wt=48868
     Region          13..417
                     /region_name="aRF1/eRF1"
                     /note="peptide chain release factor 1, archaeal and
                     eukaryotic forms; TIGR03676"
                     /db_xref="CDD:274719"
     CDS             1..436
                     /gene="eRF1"
                     /coded_by="XM_076690371.1:321..1631"
                     /db_xref="GeneID:117605069"
ORIGIN      
        1 msneetsadr nveiwkikkl ikslemargn gtsmisliip pkdqisrvsk mladefgtas
       61 niksrvnrls vlgaitsvqh rlklytkvpp nglviycgti vteegkekkv nidfepfkpi
      121 ntslylcdnk fhtealtall addnkfgfiv mdgngalfgt lqgntrevlh kftvdlpkkh
      181 grggqsalrf arlrmekrhn yvrkvaevat qlyitndkpn iaglilagsa dfktelsqsd
      241 mfdprlqakv iklvdvsygg engfnqaiel aaeslqnvkf iqekkligry fdeisqdtgk
      301 ycfgvedtlr alelgsvetl icwenldiqr yvlknhtnae ekvlhltpeq ekdkthftdk
      361 esgvelelve cqpllewlan nyksfgatle iitdksqegs qfvrgfggig gilrykvdfq
      421 slqadepldd vdlddy
//
LOCUS       XP_034190797            4085 aa            linear   INV 03-SEP-2025
DEFINITION  dynein heavy chain at 16F [Osmia lignaria lignaria].
ACCESSION   XP_034190797
VERSION     XP_034190797.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334906.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190797.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4085
                     /product="dynein heavy chain at 16F"
                     /calculated_mol_wt=470930
     Region          <603..>836
                     /region_name="HEC1"
                     /note="Chromosome segregation protein NDC80, interacts
                     with SMC proteins [Cell cycle control, cell division,
                     chromosome partitioning]; COG5185"
                     /db_xref="CDD:444066"
     Region          828..1241
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          1054..3716
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1374..1700
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          3755..4080
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..4085
                     /gene="Dhc16F"
                     /coded_by="XM_034334906.2:212..12469"
                     /db_xref="GeneID:117609059"
ORIGIN      
        1 myqnksqesl khkvneknfn elskfertdk geksltvldv asstdlkfrw crkphrnynk
       61 grdevpffkp hkkllekidy kpevrepltg fsaqqekirk qvtkpdlstk mkeekvsekd
      121 fivpkfkpvt ailerktkdl dapplvdqle liqqlrddpk igfyymvyav eqsskyftpy
      181 alkvvhykdv gdtymtisas gvtqyspdem sftpieqwer eymfylkllk irtfivfrrw
      241 kafyvwkkli tykkfveaks yikenlftsd pilskallei ksmcsiflds sffddnviek
      301 fllfyfvekq ftkleslrnk ldefrnlald ivnnaclgal llagytpdds nitieqtayg
      361 klgefsavkf kmpkdgilkm syieqarkre kcyrlscfih lidymqtnmm hnlllntyqe
      421 fvtaleihtn clpnsdllsd dvidvplten rsatnriqsp yfvvdllilp sseividpse
      481 sifrfvikti enmweenlya ikpllsdqff hsftrpminh kieericgnp pemqivlkqd
      541 pflnslktel eqildinfda vkqysqrfke iqefyledlt fdenvirdnr dcklfrdwsi
      601 rykheidlir kvidfqplgm fliqlerfkn aaelaprgkl gvieavmpgl grsrvetllt
      661 ttidainyle shpvtteeyv eyiqfvdkak qdvdemesql dyvkelydtm eefdfpipsm
      721 dmanylgigt hftslrlave nkleerpkli nkfnaqlhkd italneqise iheeimqpwl
      781 ldyesnvdac lntlkeldar lkvcattaeq yrayqktfkl eqtrfdsldq vmnelrlrvl
      841 lwesltswee avfewyagdf daldveqits ftmqtmknii qlerglpsnn ilpklkenve
      901 rvrnklpilg ylrnpdlrer hwkkieavln ykfkpdekkt wtlfenlgaf lkpnelmeva
      961 aaasseanle nmlnkvintw etlkfivipy regkdvfimg tledvqtamd esninlqtim
     1021 asrhvgpiqp vveqwvkkld lftvtleawq ylqlnwlyle aifsapdiqr qlpmeaklfi
     1081 evdrfwkdlm rrtykaplam pvathpglld lleennkmld evlkcleayl etkrvafprf
     1141 fflsndelle ilaqtknpha vqrhlqkcfd aiyrlefatk egdsnaepet vlttdivami
     1201 spegervplg kglrargnve hwlarveeam fstlrkrmke gikdlatkgr eeflymhpsq
     1261 ivlsvsqifw trnihliles senvhhkmrg feekcysdls elatmvrgel pklirdviin
     1321 litvdvhard ivttlvenrv ssstsfdwmk alryywedev dncltrmsna eylygyeylg
     1381 aqkrlvitpl tdkcylclmg alqqdlggap agpagtgkte ttkdlakava vqcvvfncse
     1441 gldyrmmgrf fsglatsgaw ccfdefnrid ievlsviaqq litirnakla ravkfmfegr
     1501 miklvmscat fitmnpgyag rtelpdnlka lfrpfsmmvp dykliaevtl ysegfesskt
     1561 lsqkmtlmyt lsseqlsqqd hydfgmravk svlvmagslk rnnpdkpeda vliralresn
     1621 ipkflhddae lfegivgdlf pgiqipeedy gilretaikv iqeanlqpee ctitkaiqlh
     1681 eclqvrhgvm lvgptgsgkt svlrtlanty nrlhqmgvpg pcyqhvhmyv inpkaitige
     1741 lygevdlmtn ewrdgligsk vrhacsftte dhqwivcdgp vdaiwienmn tvlddnkmlc
     1801 lanserikft pymrmlfevm dlaqaspatv srcgmvyvdp iqlkwmpyvk swvvtlpeds
     1861 vkaeyqtqii nlfetyfdag lvfcsrncty pigqvdiska tmtcslieci lkepeaiert
     1921 tdksrikmfl tqvfviaylw avggnvvdss rdafevfvkk qfeenedayl pstdlwdlym
     1981 nvqahrldiw qdimpeflyd sdvpffdilv ptvdtvrfgy vmkklvkank pvfftgdtgv
     2041 gksvitkvvl ksleeshvwv pitlifsaqt ssgrtqeile lklekrkrtv lgapigkrvc
     2101 vfvddvnmpk ldtygsqppi ellrqlldfr gmydreklfw kdvedvvfav acappgggrn
     2161 pltprfvrhf amllipppte islkgifkai ingfledfvd svkqigdkiv naavdvyeri
     2221 atdllptpek shyifnlrdl skciqgimqv datvvkqpne myrlfyhecl rvfhdrlinl
     2281 qdksyfykll nnicagtfgi evvrlpdqei iekpplllfg dfmafgaare qriyeeltei
     2341 pivkrtledy ledycfsvgk emqmiffmda iehvcrlari lrsergngll vgvggmgkqs
     2401 ltklashlng ykcyhievtr tydksswhed lrrfyfdpga hakhttflft dtqivleefl
     2461 edinntlntg evpnlyeade lekviiatrp aakeagiget nrdgiyqffi grvrnqlhlm
     2521 icmspigdtf rhrcrmfpsl vncctidwft kwpreallsv aenslqiivp ddsnkllals
     2581 nicvliheqv eevtvrffle mrrryyttps sylellklfl ttltkkkkki eslkskiang
     2641 lnklretnem vsvmkehlvv lapklkastd evtklvkils kqqaeadkvk yvvtaeeaia
     2701 kakaeemavl eadarqdlea ampalleaqh aletlnkndi neirvfnqpp hlvrfvmeav
     2761 nlllgektdw ataklvlsdi rfldrlityp keeisnkllt klqyyvthpe frpdlvarqs
     2821 kacksmciwv raidgyakiy rvvepkrqrl qaaeeelrsi eeevalkqke laevedkikr
     2881 lqvqydtavk nlqkleseme lvearlnrsg rltsalvder irweeatrgf drqmvnltgd
     2941 ilvaagalay lgaftneyre elvqtwlsnc ksydiktten ynlitiladp yeirlwnthg
     3001 lprdkvsten aifvtqatrw plmidpqeqa nrwirnmeqn ndlkmckltd anftrilets
     3061 irlgmpvllq evgetldpnl epillkqifi lggralirfg dtdveydnnf rlyittklan
     3121 phylpevcik vtvvnftvti ggleeqllad vvrlerpdle amrndlimki nadkgqlqni
     3181 edkiltllys sggnildned lietlndske tseiiatrli eseateekis iarekyrsva
     3241 nrgsvlyfvv anladidpmy qfslkyfnqi fntvidtsek venlqqrlqi llneitlaiy
     3301 tnvsrglfek hklvfsfmln iavhineliv sqdqwnfmlr gathiateei pdyptlttqm
     3361 wscvnylann fqtfknilnd afkkieltlg yfkteinvip tnrnvatynw dnalsdiekl
     3421 mllkalkeeq lifavtayvs knlgqkfves pmaslqllfa dtsnkiplvf ilttgsdpfs
     3481 gflrfanemg fsdkydsisl gqgqgpvaeg hlrtgliegr wvflqnchla tswmikmeql
     3541 ilqiaeepeg vihtdfrlfl ssmpstsfpv svlqnsvkvt neppkglkan ikralydldd
     3601 qffeehhlke hwrkiifgvc ffhaiiqerk kfgplgwnil yefndsdrec cllnlkifcs
     3661 ynlipwnali yttgeitygg rvtdnwdlrc mktildmffs pkilstdyvy sqsgkyycpv
     3721 yetlqeykdf vetfpiiddp evfgmhenan viyqlkeaqf ilntmlevqp qesatdqgks
     3781 ssdiayeiad miktriqlki dstkcnpnhl irdsmgrlps lttvlvhevd rynillkkih
     3841 tslenlqrai kgfvvmseel envfvafvnn qvpkmwhnvn vypslkklgs wiknlelrid
     3901 fiqiwlidtk pvsfwisgls fpqgfltgmm qtcarkyntp idhlkldfip tkvvlyqedv
     3961 eekhkefeke vievykklkv pkdgvlihgl fidagrwdfe smllvdanig emhptlpvlh
     4021 inpvlelpkn dpryvsplyk tavragvlst tghstnfvva vllpsakqqs ywilkgtall
     4081 ieiid
//
LOCUS       XP_076546487            1028 aa            linear   INV 03-SEP-2025
DEFINITION  protein nervous wreck-like [Osmia lignaria lignaria].
ACCESSION   XP_076546487
VERSION     XP_076546487.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690372.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1028
                     /product="protein nervous wreck-like"
                     /calculated_mol_wt=114860
     Region          16..281
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(21,24..25,29,31..32,35..36,38..39,49..50,52..53,
                     56..57,59..60,63,99,102..103,226,229..230,232,235,
                     238..239,241..242,245..246,249..250,252..253,256..257,
                     259..260)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          546..599
                     /region_name="SH3_FCHSD_1"
                     /note="First Src Homology 3 domain of FCH and double SH3
                     domains proteins; cd11761"
                     /db_xref="CDD:212695"
     Site            order(551,553,556,560,579..580,594,596..597)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212695"
     Region          659..714
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(664,666,669,673,695..696,709,711..712)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     CDS             1..1028
                     /gene="LOC117609097"
                     /coded_by="XM_076690372.1:363..3449"
                     /db_xref="GeneID:117609097"
ORIGIN      
        1 mqppprkgny tkflknvhae qaaklqaknq hecdlledir nftikksaie ksyseallki
       61 ssaylnkkip nipdlkvdgg eekwnmwnvw rtvleenekl ararlaavev fqqqiaddak
      121 glkmhklqis kkaidqlmiv qkelqmcvqd vdktkklyfd eehsahdvrd kakdieeklk
      181 kkkgsffqsi tslqknsakv sskrdaleek stgarndyll slaaanahqn ryfvvdlqnt
      241 mqyleqgvyd kvaeyltlmg rtelltclat qnsfgkirdq aqqltreyni qccclyypvl
      301 kqhiqyefec cdndpvdrvt adhaaaatlg kearrwstri areistiren nrkiqvllql
      361 kesgqksdpn dpqgpdidtk idelkhairr aetaklkaea rieclrnggv nvdewlqeae
      421 tlsvqdmprs asslsvrtda sgtaehpssd sfydsdgdgg sdlttverpg sarnapqqee
      481 steerqrhds eevdalveqe kqrieqltvg wddptsvdwd neekdemqta etgettepps
      541 tqqiykctal ysytaqnpde lsiveseqle vvgegdgdgw lrarnyrgee gfvpqnyldv
      601 erettsglts qgpglvqqis fssvdytidd hdavdpdanl epavtetvvq nhigepeqyc
      661 ialydydttc deelsflegd ivkvlrkeph dvddgwwege lrgqrglfps livepcaadg
      721 spltpqenit ppssappmft ppevleflle delaqnsiqe segknsnnvn teqqqqqeqq
      781 qeqqegfvin lskdqkchyg sqfegdksde gpgivvvevl eeseakinde ktkhpqslnt
      841 dqskedfglg vaqivitaat pmeeiehpfp gleetnddtv ksaenseadl psstttdmne
      901 sdckeatvim dereeqette ateateatea tevtedieek stvddlptds apfpvssssg
      961 seadstsgps tadnsqsips rgtvievqdt edieivpekr vvggrasipd elqpdqlekl
     1021 qnlkesna
//
LOCUS       XP_034190816             927 aa            linear   INV 03-SEP-2025
DEFINITION  valyl-tRNA synthetase, mitochondrial [Osmia lignaria lignaria].
ACCESSION   XP_034190816
VERSION     XP_034190816.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334925.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..927
                     /product="valyl-tRNA synthetase, mitochondrial"
                     /calculated_mol_wt=107354
     Region          25..925
                     /region_name="PTZ00419"
                     /note="valyl-tRNA synthetase-like protein; Provisional"
                     /db_xref="CDD:240411"
     CDS             1..927
                     /gene="ValRS-m"
                     /coded_by="XM_034334925.2:204..2987"
                     /db_xref="GeneID:117609064"
ORIGIN      
        1 mhiarfndll hcssykfcyc tlstqvlsdf pktfnhkaae hkwyqiwekn nyftvknnek
       61 ealkmllppp nitgtlhlgh altvivqdvl arwyrmrgyp vlwipgfdha giatqmmvek
      121 ylsktkgisk sdigreqfls hiwqwkydke dviksqikal gasldwsrey ftmskdhtna
      181 vieafvtlne rnllyrkkdl inwsptlhsa isdieverly iskktqlqvp gyekkitfge
      241 iahiaypvkd skheiivatt rpetvfgdva iavhpddery teyigkqvwh slretlipvi
      301 sdplvdkefg tgavkitpah dqldyeiatn hglniievid eygnitnagk qfkglprfia
      361 rekvlnelsn kgflrsvreh emnipicsrs hdivecmlke qwfikcksma hkalyavkqg
      421 hlniiptthe qiwydylnni rdwcisrqtw wghsipayyi tvgdkiewiv arteddarii
      481 vrkkygsdvq lqkdpdvldt wfssgilpft vlgwpketed fkqyypltll etgydilffw
      541 varmvmlgle ltnrlpfnev llhgllcday gkkmsktlgn ivspenvitg itlndltaqm
      601 kksynmgivn etilrrmlsa nkkmfpngip ecgadalrmt lcshniknqh infdimecqt
      661 nkffsnkiwq askyillmts ekkyhepksm tvidrwilsr lslmvttvnd tllqrnfhkt
      721 vaalkqflyy efcdfyleat kwgfrgenen tdishtyglr tclevflraf apimpylsdd
      781 lynqlsnklp eflsiaslme asypipeqfn kwrdvtlder inevlkmile irsfmgnink
      841 kmnpevhvvi nqsedftlyn dtinlikags kvsnisvfpe nsytqtnssv cypftcnctl
      901 fitaqdpsvl kqieqnisrk tqiskev
//
LOCUS       XP_076546488             530 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546488
VERSION     XP_076546488.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690373.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..530
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X1"
                     /calculated_mol_wt=59378
     Region          209..312
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..530
                     /gene="LOC143305712"
                     /coded_by="XM_076690373.1:4..1596"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mersanacrn elssmtndrg vshylfhqfg mqlsvkrrgq vnfqscglei ctstnwflkk
       61 lgwylsisnl yatryeigei ededlrsflk mywskmsnpa drlnyvqdss sdtetdketg
      121 grgqhriykn rmscggsskp ksclvqrdgs serhchsfns grqntnvnqq nrlcsgvgvg
      181 knqqsqnrdn lpssgsvtgi nnpqasderi tlivdntrfi vdpalftahp ntmlgrmfss
      241 gveyaqpner geyevadgis amvfraildy ykggvircpp tvavqelrea cdyllvpfda
      301 stvkcqnlrg llhelsnega rcqfevfled lilplmvnsa rrgdrechiv vlleddivdw
      361 deeyppqmge eysqtvnsta myrffkyien rdvakqvmke rglkkirlgi egyptykekv
      421 kkraggraev iynyvqrpfi hmswekeeak srhvdfqcvk sksvtnlaea tadpvldagg
      481 npiaigllqa adpvpqpeiv lpvgsdadve gaiglptdqd lgsippdelp
//
LOCUS       XP_076546489             529 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546489
VERSION     XP_076546489.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690374.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..529
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X2"
                     /calculated_mol_wt=59197
     Region          208..311
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..529
                     /gene="LOC143305712"
                     /coded_by="XM_076690374.1:5..1594"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mersanacrn elssmtndrg vshylfhqfg mqlsvkrrgq vnfqscglei ctstnwflkk
       61 lgwylsisnl yatryeigei ededlrsflk iwskmsnpad rlnyvqdsss dtetdketgg
      121 rgqhriyknr mscggsskpk sclvqrdgss erhchsfnsg rqntnvnqqn rlcsgvgvgk
      181 nqqsqnrdnl pssgsvtgin npqasderit livdntrfiv dpalftahpn tmlgrmfssg
      241 veyaqpnerg eyevadgisa mvfraildyy kggvircppt vavqelreac dyllvpfdas
      301 tvkcqnlrgl lhelsnegar cqfevfledl ilplmvnsar rgdrechivv lleddivdwd
      361 eeyppqmgee ysqtvnstam yrffkyienr dvakqvmker glkkirlgie gyptykekvk
      421 kraggraevi ynyvqrpfih mswekeeaks rhvdfqcvks ksvtnlaeat adpvldaggn
      481 piaigllqaa dpvpqpeivl pvgsdadveg aiglptdqdl gsippdelp
//
LOCUS       XP_076546490             523 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546490
VERSION     XP_076546490.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690375.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..523
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X3"
                     /calculated_mol_wt=58587
     Region          202..305
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..523
                     /gene="LOC143305712"
                     /coded_by="XM_076690375.1:5..1576"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mersanacrn elssmtndrg vshylfhqfg mqlsvkrrgq vnfqscglei ctstnwflkk
       61 lgwyatryei geiededlrs flkmywskms npadrlnyvq dsssdtetdk etggrgqhri
      121 yknrmscggs skpksclvqr dgsserhchs fnsgrqntnv nqqnrlcsgv gvgknqqsqn
      181 rdnlpssgsv tginnpqasd eritlivdnt rfivdpalft ahpntmlgrm fssgveyaqp
      241 nergeyevad gisamvfrai ldyykggvir cpptvavqel reacdyllvp fdastvkcqn
      301 lrgllhelsn egarcqfevf ledlilplmv nsarrgdrec hivvlleddi vdwdeeyppq
      361 mgeeysqtvn stamyrffky ienrdvakqv mkerglkkir lgiegyptyk ekvkkraggr
      421 aeviynyvqr pfihmsweke eaksrhvdfq cvksksvtnl aeatadpvld aggnpiaigl
      481 lqaadpvpqp eivlpvgsda dvegaiglpt dqdlgsippd elp
//
LOCUS       XP_076546491             508 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546491
VERSION     XP_076546491.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690376.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X4"
                     /calculated_mol_wt=56688
     Region          187..290
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..508
                     /gene="LOC143305712"
                     /coded_by="XM_076690376.1:4..1530"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mersanacrn elssmtndrg vshylfhqfg mqlsvkrrgq vnfqscglei ctstnwflkk
       61 lgwylsisnf wskmsnpadr lnyvqdsssd tetdketggr gqhriyknrm scggsskpks
      121 clvqrdgsse rhchsfnsgr qntnvnqqnr lcsgvgvgkn qqsqnrdnlp ssgsvtginn
      181 pqasderitl ivdntrfivd palftahpnt mlgrmfssgv eyaqpnerge yevadgisam
      241 vfraildyyk ggvircpptv avqelreacd yllvpfdast vkcqnlrgll helsnegarc
      301 qfevfledli lplmvnsarr gdrechivvl leddivdwde eyppqmgeey sqtvnstamy
      361 rffkyienrd vakqvmkerg lkkirlgieg yptykekvkk raggraeviy nyvqrpfihm
      421 swekeeaksr hvdfqcvksk svtnlaeata dpvldaggnp iaigllqaad pvpqpeivlp
      481 vgsdadvega iglptdqdlg sippdelp
//
LOCUS       XP_076546492             503 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546492
VERSION     XP_076546492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690377.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..503
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X5"
                     /calculated_mol_wt=56273
     Region          182..285
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..503
                     /gene="LOC143305712"
                     /coded_by="XM_076690377.1:182..1693"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mfgmqlsvkr rgqvnfqscg leictstnwf lkklgwylsi snlyatryei geiededlrs
       61 flkmywskms npadrlnyvq dsssdtetdk etggrgqhri yknrmscggs skpksclvqr
      121 dgsserhchs fnsgrqntnv nqqnrlcsgv gvgknqqsqn rdnlpssgsv tginnpqasd
      181 eritlivdnt rfivdpalft ahpntmlgrm fssgveyaqp nergeyevad gisamvfrai
      241 ldyykggvir cpptvavqel reacdyllvp fdastvkcqn lrgllhelsn egarcqfevf
      301 ledlilplmv nsarrgdrec hivvlleddi vdwdeeyppq mgeeysqtvn stamyrffky
      361 ienrdvakqv mkerglkkir lgiegyptyk ekvkkraggr aeviynyvqr pfihmsweke
      421 eaksrhvdfq cvksksvtnl aeatadpvld aggnpiaigl lqaadpvpqp eivlpvgsda
      481 dvegaiglpt dqdlgsippd elp
//
LOCUS       XP_076546493             500 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546493
VERSION     XP_076546493.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690378.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..500
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X6"
                     /calculated_mol_wt=55937
     Region          179..282
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..500
                     /gene="LOC143305712"
                     /coded_by="XM_076690378.1:135..1637"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mqlsvkrrgq vnfqscglei ctstnwflkk lgwylsisnl yatryeigei ededlrsflk
       61 mywskmsnpa drlnyvqdss sdtetdketg grgqhriykn rmscggsskp ksclvqrdgs
      121 serhchsfns grqntnvnqq nrlcsgvgvg knqqsqnrdn lpssgsvtgi nnpqasderi
      181 tlivdntrfi vdpalftahp ntmlgrmfss gveyaqpner geyevadgis amvfraildy
      241 ykggvircpp tvavqelrea cdyllvpfda stvkcqnlrg llhelsnega rcqfevfled
      301 lilplmvnsa rrgdrechiv vlleddivdw deeyppqmge eysqtvnsta myrffkyien
      361 rdvakqvmke rglkkirlgi egyptykekv kkraggraev iynyvqrpfi hmswekeeak
      421 srhvdfqcvk sksvtnlaea tadpvldagg npiaigllqa adpvpqpeiv lpvgsdadve
      481 gaiglptdqd lgsippdelp
//
LOCUS       XP_076546494             496 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546494
VERSION     XP_076546494.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690379.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X7"
                     /calculated_mol_wt=55482
     Region          175..278
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..496
                     /gene="LOC143305712"
                     /coded_by="XM_076690379.1:183..1673"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mfgmqlsvkr rgqvnfqscg leictstnwf lkklgwyatr yeigeieded lrsflkmyws
       61 kmsnpadrln yvqdsssdte tdketggrgq hriyknrmsc ggsskpkscl vqrdgsserh
      121 chsfnsgrqn tnvnqqnrlc sgvgvgknqq sqnrdnlpss gsvtginnpq asderitliv
      181 dntrfivdpa lftahpntml grmfssgvey aqpnergeye vadgisamvf raildyykgg
      241 vircpptvav qelreacdyl lvpfdastvk cqnlrgllhe lsnegarcqf evfledlilp
      301 lmvnsarrgd rechivvlle ddivdwdeey ppqmgeeysq tvnstamyrf fkyienrdva
      361 kqvmkerglk kirlgiegyp tykekvkkra ggraeviyny vqrpfihmsw ekeeaksrhv
      421 dfqcvksksv tnlaeatadp vldaggnpia igllqaadpv pqpeivlpvg sdadvegaig
      481 lptdqdlgsi ppdelp
//
LOCUS       XP_076546495             491 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546495
VERSION     XP_076546495.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690380.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..491
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X8"
                     /calculated_mol_wt=55161
     Region          170..273
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..491
                     /gene="LOC143305712"
                     /coded_by="XM_076690380.1:4..1479"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mersanacrn elssmtndrg vshylfhqfg mqlsvkrrgq vnfqscglei ctstnwflkk
       61 lgwylsisnl yatryeigei ededlrsflk mywskmsnpa drlnyvqdss sdtetdketg
      121 grgqhriykn rmrlcsgvgv gknqqsqnrd nlpssgsvtg innpqasder itlivdntrf
      181 ivdpalftah pntmlgrmfs sgveyaqpne rgeyevadgi samvfraild yykggvircp
      241 ptvavqelre acdyllvpfd astvkcqnlr gllhelsneg arcqfevfle dlilplmvns
      301 arrgdrechi vvlleddivd wdeeyppqmg eeysqtvnst amyrffkyie nrdvakqvmk
      361 erglkkirlg iegyptykek vkkraggrae viynyvqrpf ihmswekeea ksrhvdfqcv
      421 ksksvtnlae atadpvldag gnpiaigllq aadpvpqpei vlpvgsdadv egaiglptdq
      481 dlgsippdel p
//
LOCUS       XP_076546496             481 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X9 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546496
VERSION     XP_076546496.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690381.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X9"
                     /calculated_mol_wt=53583
     Region          160..263
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..481
                     /gene="LOC143305712"
                     /coded_by="XM_076690381.1:181..1626"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mfgmqlsvkr rgqvnfqscg leictstnwf lkklgwylsi snfwskmsnp adrlnyvqds
       61 ssdtetdket ggrgqhriyk nrmscggssk pksclvqrdg sserhchsfn sgrqntnvnq
      121 qnrlcsgvgv gknqqsqnrd nlpssgsvtg innpqasder itlivdntrf ivdpalftah
      181 pntmlgrmfs sgveyaqpne rgeyevadgi samvfraild yykggvircp ptvavqelre
      241 acdyllvpfd astvkcqnlr gllhelsneg arcqfevfle dlilplmvns arrgdrechi
      301 vvlleddivd wdeeyppqmg eeysqtvnst amyrffkyie nrdvakqvmk erglkkirlg
      361 iegyptykek vkkraggrae viynyvqrpf ihmswekeea ksrhvdfqcv ksksvtnlae
      421 atadpvldag gnpiaigllq aadpvpqpei vlpvgsdadv egaiglptdq dlgsippdel
      481 p
//
LOCUS       XP_076546497             478 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X10 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546497
VERSION     XP_076546497.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690382.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X10"
                     /calculated_mol_wt=53247
     Region          157..260
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..478
                     /gene="LOC143305712"
                     /coded_by="XM_076690382.1:135..1571"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mqlsvkrrgq vnfqscglei ctstnwflkk lgwylsisnf wskmsnpadr lnyvqdsssd
       61 tetdketggr gqhriyknrm scggsskpks clvqrdgsse rhchsfnsgr qntnvnqqnr
      121 lcsgvgvgkn qqsqnrdnlp ssgsvtginn pqasderitl ivdntrfivd palftahpnt
      181 mlgrmfssgv eyaqpnerge yevadgisam vfraildyyk ggvircpptv avqelreacd
      241 yllvpfdast vkcqnlrgll helsnegarc qfevfledli lplmvnsarr gdrechivvl
      301 leddivdwde eyppqmgeey sqtvnstamy rffkyienrd vakqvmkerg lkkirlgieg
      361 yptykekvkk raggraeviy nyvqrpfihm swekeeaksr hvdfqcvksk svtnlaeata
      421 dpvldaggnp iaigllqaad pvpqpeivlp vgsdadvega iglptdqdlg sippdelp
//
LOCUS       XP_076546498             469 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein KCTD20-like isoform X11 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546498
VERSION     XP_076546498.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690383.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="BTB/POZ domain-containing protein KCTD20-like
                     isoform X11"
                     /calculated_mol_wt=52471
     Region          148..251
                     /region_name="BTB_3"
                     /note="BTB/POZ domain; pfam16017"
                     /db_xref="CDD:464976"
     CDS             1..469
                     /gene="LOC143305712"
                     /coded_by="XM_076690383.1:4..1413"
                     /db_xref="GeneID:143305712"
ORIGIN      
        1 mersanacrn elssmtndrg vshylfhqfg mqlsvkrrgq vnfqscglei ctstnwflkk
       61 lgwylsisnf wskmsnpadr lnyvqdsssd tetdketggr gqhriyknrm rlcsgvgvgk
      121 nqqsqnrdnl pssgsvtgin npqasderit livdntrfiv dpalftahpn tmlgrmfssg
      181 veyaqpnerg eyevadgisa mvfraildyy kggvircppt vavqelreac dyllvpfdas
      241 tvkcqnlrgl lhelsnegar cqfevfledl ilplmvnsar rgdrechivv lleddivdwd
      301 eeyppqmgee ysqtvnstam yrffkyienr dvakqvmker glkkirlgie gyptykekvk
      361 kraggraevi ynyvqrpfih mswekeeaks rhvdfqcvks ksvtnlaeat adpvldaggn
      421 piaigllqaa dpvpqpeivl pvgsdadveg aiglptdqdl gsippdelp
//
LOCUS       XP_034190828             525 aa            linear   INV 03-SEP-2025
DEFINITION  kelch domain-containing protein 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190828
VERSION     XP_034190828.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334937.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="kelch domain-containing protein 4 isoform X1"
                     /calculated_mol_wt=60033
     Region          69..120
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          <81..257
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          124..179
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          182..235
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          302..354
                     /region_name="Kelch_4"
                     /note="Galactose oxidase, central domain; pfam13418"
                     /db_xref="CDD:433191"
     CDS             1..525
                     /gene="LOC117609071"
                     /coded_by="XM_034334937.2:369..1946"
                     /db_xref="GeneID:117609071"
ORIGIN      
        1 mgkkdknkkk isgnvktalk tekkqnakqk kelaargedd iehviaeier eeakrkciie
       61 ktvappsrri nctltahpfk deliifggef hdgrttfvyg dmfiyslskn ewtiikapgs
      121 ppprcshqav tttankgelw ifggeftsps esqfyhyrdl wvyrfaekkw ekisspggps
      181 arsghrmtli kkqlivfggf hdnlrdykyy ndvhifnldt ytwhkielsg tpplprsgci
      241 mlptpdnkll iyggyskeki kkdvdkgyvh ndmflmtpek ndqtglkwkw vlvkqsgiki
      301 sprcsvsavl vqpnlaylfg gvfdneddee elhgtfyndl valdlekfqw rivalhgkkd
      361 ittrrrrrkp kedggqends eneddnhsem qepssvptes itthddgift vtigpartsv
      421 tqeqeitqkd vfmpcprina alavkhnily lhggmfedgd rqytlndfys ldfrkldewr
      481 timsddlstq twfdasssds dseeiddsdg senesedehm diden
//
LOCUS       XP_034190846             496 aa            linear   INV 03-SEP-2025
DEFINITION  kelch domain-containing protein 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190846
VERSION     XP_034190846.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334955.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="kelch domain-containing protein 4 isoform X2"
                     /calculated_mol_wt=56747
     Region          40..91
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          <52..228
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          95..150
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          153..206
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          273..325
                     /region_name="Kelch_4"
                     /note="Galactose oxidase, central domain; pfam13418"
                     /db_xref="CDD:433191"
     CDS             1..496
                     /gene="LOC117609071"
                     /coded_by="XM_034334955.2:45..1535"
                     /db_xref="GeneID:117609071"
ORIGIN      
        1 mqgckgckdd diehviaeie reeakrkcii ektvappsrr inctltahpf kdeliifgge
       61 fhdgrttfvy gdmfiyslsk newtiikapg sppprcshqa vtttankgel wifggeftsp
      121 sesqfyhyrd lwvyrfaekk wekisspggp sarsghrmtl ikkqlivfgg fhdnlrdyky
      181 yndvhifnld tytwhkiels gtpplprsgc imlptpdnkl liyggyskek ikkdvdkgyv
      241 hndmflmtpe kndqtglkwk wvlvkqsgik isprcsvsav lvqpnlaylf ggvfdnedde
      301 eelhgtfynd lvaldlekfq wrivalhgkk dittrrrrrk pkedggqend seneddnhse
      361 mqepssvpte sitthddgif tvtigparts vtqeqeitqk dvfmpcprin aalavkhnil
      421 ylhggmfedg drqytlndfy sldfrkldew rtimsddlst qtwfdasssd sdseeiddsd
      481 gsenesedeh mdiden
//
LOCUS       XP_076546499             496 aa            linear   INV 03-SEP-2025
DEFINITION  kelch domain-containing protein 4 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546499
VERSION     XP_076546499.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690384.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="kelch domain-containing protein 4 isoform X3"
                     /calculated_mol_wt=56747
     Region          40..91
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          <52..228
                     /region_name="NanM"
                     /note="N-acetylneuraminic acid mutarotase [Cell
                     wall/membrane/envelope biogenesis]; COG3055"
                     /db_xref="CDD:442289"
     Region          95..150
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          153..206
                     /region_name="KELCH repeat"
                     /note="KELCH repeat [structural motif]"
                     /db_xref="CDD:276965"
     Region          273..325
                     /region_name="Kelch_4"
                     /note="Galactose oxidase, central domain; pfam13418"
                     /db_xref="CDD:433191"
     CDS             1..496
                     /gene="LOC117609071"
                     /coded_by="XM_076690384.1:16..1506"
                     /db_xref="GeneID:117609071"
ORIGIN      
        1 mqgckgckdd diehviaeie reeakrkcii ektvappsrr inctltahpf kdeliifgge
       61 fhdgrttfvy gdmfiyslsk newtiikapg sppprcshqa vtttankgel wifggeftsp
      121 sesqfyhyrd lwvyrfaekk wekisspggp sarsghrmtl ikkqlivfgg fhdnlrdyky
      181 yndvhifnld tytwhkiels gtpplprsgc imlptpdnkl liyggyskek ikkdvdkgyv
      241 hndmflmtpe kndqtglkwk wvlvkqsgik isprcsvsav lvqpnlaylf ggvfdnedde
      301 eelhgtfynd lvaldlekfq wrivalhgkk dittrrrrrk pkedggqend seneddnhse
      361 mqepssvpte sitthddgif tvtigparts vtqeqeitqk dvfmpcprin aalavkhnil
      421 ylhggmfedg drqytlndfy sldfrkldew rtimsddlst qtwfdasssd sdseeiddsd
      481 gsenesedeh mdiden
//
LOCUS       XP_034190868             255 aa            linear   INV 03-SEP-2025
DEFINITION  hydroxysteroid 17-beta dehydrogenase 10 [Osmia lignaria lignaria].
ACCESSION   XP_034190868
VERSION     XP_034190868.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334977.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190868.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="hydroxysteroid 17-beta dehydrogenase 10"
                     /calculated_mol_wt=27149
     Region          3..255
                     /region_name="HSD10-like_SDR_c"
                     /note="17hydroxysteroid dehydrogenase type 10
                     (HSD10)-like, classical (c) SDRs; cd05371"
                     /db_xref="CDD:187629"
     Site            order(11,14..16,35..36,57..59,85..87,114,147..149,162,166,
                     192..195,197..200)
                     /site_type="other"
                     /note="NAD binding site [chemical binding]"
                     /db_xref="CDD:187629"
     Site            order(93..95,97,102..103,105,108..109,112,116..117,
                     120..121,124,127..128,131,138..140,152..158,160,163..164,
                     167..168,171..172,174..176,178..179,181..184,186,195,
                     216..218,220..222,226,229..230,233,235..250,252..255)
                     /site_type="other"
                     /note="homotetramer interface [polypeptide binding]"
                     /db_xref="CDD:187629"
     Site            order(93..95,102..103,105,108,112,117,120..121,124,
                     152..158,160,163..164,167..168,171..172,175..176,178..179,
                     253..255)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:187629"
     Site            order(115,149,162,166)
                     /site_type="active"
                     /db_xref="CDD:187629"
     CDS             1..255
                     /gene="scu"
                     /coded_by="XM_034334977.2:153..920"
                     /db_xref="GeneID:117609087"
ORIGIN      
        1 mlkgmvalvt ggasglglgt vqrfvregak vviadlptsk gnevattlgd savfspmdvt
       61 serdvnatvq liekqfekln vvvnaagvac aykvynpnsk lphkledfqr vinvnavgtf
      121 nvirlclpfm hnnkpdddgq rgviintasv aafdgqigqa aysaskgaiv gmtlplardl
      181 sklgvrvvti apglfdtpll gslpekvrvf lmktvpfpsr lgkpdeyaql aqhivenrll
      241 ngevirldga lrmqp
//
LOCUS       XP_034190882             245 aa            linear   INV 03-SEP-2025
DEFINITION  nurim homolog [Osmia lignaria lignaria].
ACCESSION   XP_034190882
VERSION     XP_034190882.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334991.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..245
                     /product="nurim homolog"
                     /calculated_mol_wt=28813
     CDS             1..245
                     /gene="LOC117609091"
                     /coded_by="XM_034334991.2:187..924"
                     /db_xref="GeneID:117609091"
ORIGIN      
        1 mfvkcisvvt caacflytfy ilcilsyfls thnenkekii slkkdenvds vlwllivnms
       61 llstfmlqhs lmasdfvkhl fckfhmdyle rsiynvtsam alhllfnqwq iispitlwnv
      121 ntssnstawf iftalhvlaw siiysgclmm diselagikq vyykfssrps pmvmkskell
      181 ryyshmrhps ftgfliilwi ypymtldrll laliltvymi lmwtidkedy nyhatvvkrk
      241 qrelf
//
LOCUS       XP_034190912             292 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609115 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190912
VERSION     XP_034190912.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190912.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="uncharacterized protein LOC117609115 isoform X1"
                     /calculated_mol_wt=32410
     Region          <83..135
                     /region_name="DUF308"
                     /note="Short repeat of unknown function (DUF308);
                     pfam03729"
                     /db_xref="CDD:427468"
     CDS             1..292
                     /gene="LOC117609115"
                     /coded_by="XM_034335021.2:516..1394"
                     /db_xref="GeneID:117609115"
ORIGIN      
        1 mhavglhsal aikrqrkrrd eqrrarerry saqsgesglt sprastdsld hpprhhkhgh
       61 pnrsagqgml dtkvvtsigm lhigvvflvl ggfllmsgll pgdlaqwgtk asggwwnelv
      121 aiglfaiivg iflivlnrvi akreendlee yvqrqltrsr sghrlerdve tgglstkhar
      181 rarqmqqmia sevesiesqn ekesegsppr spppaysppp tmngdhqtaq stlfleqite
      241 eeiisdrvet sttnslspgs psetrellqn prhskqngnp qqvhrplyvs ri
//
LOCUS       XP_034190919             204 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609115 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190919
VERSION     XP_034190919.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335028.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..204
                     /product="uncharacterized protein LOC117609115 isoform X2"
                     /calculated_mol_wt=21998
     CDS             1..204
                     /gene="LOC117609115"
                     /coded_by="XM_034335028.2:520..1134"
                     /db_xref="GeneID:117609115"
ORIGIN      
        1 migampavav rherkrqekr lkrpsqlylg gtwmpppqss pptpsphgpn shleplpely
       61 lcgkisglha vvvclllgav vlvvglvqla pgatttdhrl allvagtill llgiilaavr
      121 cyvlhcvplp tespvatpvp ppaneqaglp rgpldlligh qnqpendalm qtdhrhqhhh
      181 hhhhhhhgnh kkkrssqgsh sdea
//
LOCUS       XP_034173171             260 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolar protein viriato [Osmia lignaria lignaria].
ACCESSION   XP_034173171
VERSION     XP_034173171.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317280.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="nucleolar protein viriato"
                     /calculated_mol_wt=30496
     Region          39..>131
                     /region_name="Nop25"
                     /note="Nucleolar protein 12 (25kDa); pfam09805"
                     /db_xref="CDD:430839"
     CDS             1..260
                     /gene="vito"
                     /coded_by="XM_034317280.2:9..791"
                     /db_xref="GeneID:117601040"
ORIGIN      
        1 mfnlvgsgvl giyinvctmv qtkmqpklln vnriphrpkk rrkitvvfde kkrrdflkgf
       61 rkrklqrkek aaeklqqqlk eerkkikqgv reeykslvss rdipeiqqll sqheyetegh
      121 tisilelnva dltdnalige nksnieteen ekeesdnnse deeeiegmpl nektksttsk
      181 ktienklign qkdlkkaikk atlkqvkksk vfqrkqkler qknkkesmrk qkrnekirkh
      241 ngklkkklnd qmiyiaskif
//
LOCUS       XP_034190933             229 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609125 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190933
VERSION     XP_034190933.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335042.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..229
                     /product="uncharacterized protein LOC117609125 isoform X1"
                     /calculated_mol_wt=24571
     CDS             1..229
                     /gene="LOC117609125"
                     /coded_by="XM_034335042.2:9..698"
                     /db_xref="GeneID:117609125"
ORIGIN      
        1 mksqtrgtpk ipilwlysan fvelicsdtt dsdgvirktv lpvietsfai ligkmitalp
       61 vpsvkvfhkt yyqekgarit ssqpgsplte seavlalmdk eppeyyfcgk vnslhvvvgs
      121 lllgavvlvv glvqlapgae aaqnstalia vgcsllvvgv llaplralci kkqkaaqkdg
      181 thqrsvtsid mllaqhrdft vltpdeledl vgrptsnlen kirkqghnt
//
LOCUS       XP_034190942             175 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609125 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190942
VERSION     XP_034190942.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335051.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="uncharacterized protein LOC117609125 isoform X2"
                     /calculated_mol_wt=18621
     CDS             1..175
                     /gene="LOC117609125"
                     /coded_by="XM_034335051.2:184..711"
                     /db_xref="GeneID:117609125"
ORIGIN      
        1 mitalpvpsv kvfhktyyqe kgaritssqp gsplteseav lalmdkeppe yyfcgkvnsl
       61 hvvvgslllg avvlvvglvq lapgaeaaqn staliavgcs llvvgvllap lralcikkqk
      121 aaqkdgthqr svtsidmlla qhrdftvltp deledlvgrp tsnlenkirk qghnt
//
LOCUS       XP_034190953             227 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609132 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190953
VERSION     XP_034190953.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335062.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190953.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..227
                     /product="uncharacterized protein LOC117609132 isoform X1"
                     /calculated_mol_wt=24113
     CDS             1..227
                     /gene="LOC117609132"
                     /coded_by="XM_034335062.2:357..1040"
                     /db_xref="GeneID:117609132"
ORIGIN      
        1 mpdsgavyqp ttvgytydsg gdgaggsggl rngfwriisr hklssrrwfe wvllsvavsf
       61 fvaglttmlv sfisdgtsqe nkfqikmnep sttsatniee qvedaakssi algagmmlig
      121 lllgfvwawl rffhhgkspr sgmvgssgqm lgglnpstdl lvgstsqygp vltelpsqlk
      181 lkqttphdvs aiplsdqeee trtlmqdsvi psavssgpnt ianqipg
//
LOCUS       XP_034190963             209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609132 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190963
VERSION     XP_034190963.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335072.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190963.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="uncharacterized protein LOC117609132 isoform X2"
                     /calculated_mol_wt=22341
     CDS             1..209
                     /gene="LOC117609132"
                     /coded_by="XM_034335072.2:360..989"
                     /db_xref="GeneID:117609132"
ORIGIN      
        1 mpdsgavyqp ttvgytydsg gdgaggsggl rngfwriisr hklssrrwfe wvllsvavsf
       61 fvaglttmlv sfisdgtsqe nkfqikmnep sttsatniee qvedaakssi algagmmlig
      121 lllgfvwawl rffhhgkspr sgmvgssgqy gpvltelpsq lklkqttphd vsaiplsdqe
      181 eetrtlmqds vipsavssgp ntianqipg
//
LOCUS       XP_034190737             959 aa            linear   INV 03-SEP-2025
DEFINITION  transcription initiation factor TFIID subunit 4 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190737
VERSION     XP_034190737.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334846.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..959
                     /product="transcription initiation factor TFIID subunit 4
                     isoform X1"
                     /calculated_mol_wt=101687
     Region          <135..>394
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          465..550
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          712..956
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..959
                     /gene="LOC117609015"
                     /coded_by="XM_034334846.2:618..3497"
                     /db_xref="GeneID:117609015"
ORIGIN      
        1 masakfleea lstdvdesav naivgsletq lvtstpavsg hqvssasvsq qnhqvnsgis
       61 nggtitavqs qkhgvsnggg attvnslmtq evtksitast lhpvnvvtsn tvapqvttqq
      121 qqqhtqsavp paayinqvtt nqvtsnqttn ipsltkphep vkiiyptvgq viattgvana
      181 nnklsfpaqt vgqlangtlg itsqavlqtt snvsnvgsvs etgsqtvasv nkptgtalvi
      241 ktsvapsgmv svpmsvpvsv agnavstalq gkagvtstiv psnvqilnvn tmrpgtpvag
      301 qqagkqvapr vvigqhmlgt rpgapgitlq tlhglqpgtq ghillktesg qyqllrvgpp
      361 ptagtpagta vtpnsiagna vvaapspgtt yrlasvpavs rlntqftgpp latlrksvqt
      421 vaatittatt tpatvtaaat ttptppvttv qtvqtpaprq ttdntkekcr kflanllels
      481 srepkavern vrtliqelid tkvepeefcd rlerllnasp qpcligflkk slpllrqslv
      541 tkelviegik pppvnvvfpv tsatpvvtqq nqlrptvava aatipvtaat attqvrvmtp
      601 lpaatttvpr ppqpvqqrlm rpvttvvrsp saytvkstva vtgirptvpt vqkppiattv
      661 vktvttttqg ktinttttvn kavvpsfvpk pvikekekkt fssagytgdd dindvaamgg
      721 vnlaeesqri lgstefvgtq irsckdevfl hmtplqqrik qivsnyglee pnqevaalis
      781 haaqerlknl veklaviaeh ridlikvdpr yevtqdvraq lkfledldkv errrheeqer
      841 elllraaksr aktedpeqak lkakakemqr aemeelrqre anltalqaig prkkpkldvg
      901 gsasspgsnv glnasatgin rqmpmrprlk rvnfrdllfl leqeketcrs tmlyksylk
//
LOCUS       XP_034190743             958 aa            linear   INV 03-SEP-2025
DEFINITION  transcription initiation factor TFIID subunit 4 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190743
VERSION     XP_034190743.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334852.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..958
                     /product="transcription initiation factor TFIID subunit 4
                     isoform X2"
                     /calculated_mol_wt=101559
     Region          <135..>394
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          465..550
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          711..955
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..958
                     /gene="LOC117609015"
                     /coded_by="XM_034334852.2:687..3563"
                     /db_xref="GeneID:117609015"
ORIGIN      
        1 masakfleea lstdvdesav naivgsletq lvtstpavsg hqvssasvsq qnhqvnsgis
       61 nggtitavqs qkhgvsnggg attvnslmtq evtksitast lhpvnvvtsn tvapqvttqq
      121 qqqhtqsavp paayinqvtt nqvtsnqttn ipsltkphep vkiiyptvgq viattgvana
      181 nnklsfpaqt vgqlangtlg itsqavlqtt snvsnvgsvs etgsqtvasv nkptgtalvi
      241 ktsvapsgmv svpmsvpvsv agnavstalq gkagvtstiv psnvqilnvn tmrpgtpvag
      301 qqagkqvapr vvigqhmlgt rpgapgitlq tlhglqpgtq ghillktesg qyqllrvgpp
      361 ptagtpagta vtpnsiagna vvaapspgtt yrlasvpavs rlntqftgpp latlrksvqt
      421 vaatittatt tpatvtaaat ttptppvttv qtvqtpaprq ttdntkekcr kflanllels
      481 srepkavern vrtliqelid tkvepeefcd rlerllnasp qpcligflkk slpllrqslv
      541 tkelviegik pppvnvvfpv tsatpvvtqn qlrptvavaa atipvtaata ttqvrvmtpl
      601 paatttvprp pqpvqqrlmr pvttvvrsps aytvkstvav tgirptvptv qkppiattvv
      661 ktvttttqgk tinttttvnk avvpsfvpkp vikekekktf ssagytgddd indvaamggv
      721 nlaeesqril gstefvgtqi rsckdevflh mtplqqrikq ivsnygleep nqevaalish
      781 aaqerlknlv eklaviaehr idlikvdpry evtqdvraql kfledldkve rrrheeqere
      841 lllraaksra ktedpeqakl kakakemqra emeelrqrea nltalqaigp rkkpkldvgg
      901 sasspgsnvg lnasatginr qmpmrprlkr vnfrdllfll eqeketcrst mlyksylk
//
LOCUS       XP_034190752             938 aa            linear   INV 03-SEP-2025
DEFINITION  transcription initiation factor TFIID subunit 4 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190752
VERSION     XP_034190752.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334861.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..938
                     /product="transcription initiation factor TFIID subunit 4
                     isoform X3"
                     /calculated_mol_wt=99391
     Region          <135..>394
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          444..529
                     /region_name="TAFH"
                     /note="NHR1 homology to TAF; pfam07531"
                     /db_xref="CDD:462195"
     Region          691..935
                     /region_name="TAF4"
                     /note="Transcription initiation factor TFIID component
                     TAF4 family; pfam05236"
                     /db_xref="CDD:461598"
     CDS             1..938
                     /gene="LOC117609015"
                     /coded_by="XM_034334861.2:687..3503"
                     /db_xref="GeneID:117609015"
ORIGIN      
        1 masakfleea lstdvdesav naivgsletq lvtstpavsg hqvssasvsq qnhqvnsgis
       61 nggtitavqs qkhgvsnggg attvnslmtq evtksitast lhpvnvvtsn tvapqvttqq
      121 qqqhtqsavp paayinqvtt nqvtsnqttn ipsltkphep vkiiyptvgq viattgvana
      181 nnklsfpaqt vgqlangtlg itsqavlqtt snvsnvgsvs etgsqtvasv nkptgtalvi
      241 ktsvapsgmv svpmsvpvsv agnavstalq gkagvtstiv psnvqilnvn tmrpgtpvag
      301 qqagkqvapr vvigqhmlgt rpgapgitlq tlhglqpgtq ghillktesg qyqllrvgpp
      361 ptagtpagta vtpnsiagna vvaapspgtt yrlasvpatv aatittattt patvtaaatt
      421 tptppvttvq tvqtpaprqt tdntkekcrk flanllelss repkavernv rtliqelidt
      481 kvepeefcdr lerllnaspq pcligflkks lpllrqslvt kelviegikp ppvnvvfpvt
      541 satpvvtqqn qlrptvavaa atipvtaata ttqvrvmtpl paatttvprp pqpvqqrlmr
      601 pvttvvrsps aytvkstvav tgirptvptv qkppiattvv ktvttttqgk tinttttvnk
      661 avvpsfvpkp vikekekktf ssagytgddd indvaamggv nlaeesqril gstefvgtqi
      721 rsckdevflh mtplqqrikq ivsnygleep nqevaalish aaqerlknlv eklaviaehr
      781 idlikvdpry evtqdvraql kfledldkve rrrheeqere lllraaksra ktedpeqakl
      841 kakakemqra emeelrqrea nltalqaigp rkkpkldvgg sasspgsnvg lnasatginr
      901 qmpmrprlkr vnfrdllfll eqeketcrst mlyksylk
//
LOCUS       XP_034190762             429 aa            linear   INV 03-SEP-2025
DEFINITION  peptidoglycan-recognition protein LC isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190762
VERSION     XP_034190762.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334871.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..429
                     /product="peptidoglycan-recognition protein LC isoform X1"
                     /calculated_mol_wt=47348
     Region          266..405
                     /region_name="PGRP"
                     /note="Animal peptidoglycan recognition proteins
                     homologous to Bacteriophage T3 lysozyme; smart00701"
                     /db_xref="CDD:128941"
     Site            order(293,328,402,408,410)
                     /site_type="active"
                     /note="amidase catalytic site [active]"
                     /db_xref="CDD:133475"
     Site            order(293,402,410)
                     /site_type="other"
                     /note="Zn binding residues [ion binding]"
                     /db_xref="CDD:133475"
     Site            order(294..295,324,328,342,349..350,355,402,406,408..410)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:133475"
     CDS             1..429
                     /gene="LOC117609031"
                     /coded_by="XM_034334871.2:1000..2289"
                     /db_xref="GeneID:117609031"
ORIGIN      
        1 mgmvtlvqhh qetapdedry qkdqdqvini ptenrdsddc reeiahplkd tavvvagngs
       61 lagsstqcsd veeeddedet dsderewipd lpvptvyqqh qqvstadgnv vlpnaeasnf
      121 gdvrvknssn vhlgnktfyk gpvtikqfvy tnpvsvqeng nvkddneqth dangsassaa
      181 nnkedstvns svlsqsrnid kvtkwlwtwr caalmctlsl ilvttviavs vhytsnstap
      241 papifpeipd sslgvagvkv knvrfverse wgaqppsvql tkmklpvpyv iishtatefc
      301 ttqsectfhv rfaqtfhies rrwsdigynf lvggdgyvyv grswdymgsh afgfnnisig
      361 isfigtfnsv ippknqlyaa qrvielgveh gkispdykll ghrqvsrtls pgdalysiiq
      421 twphwsstp
//
LOCUS       XP_034190768             427 aa            linear   INV 03-SEP-2025
DEFINITION  peptidoglycan-recognition protein LC isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190768
VERSION     XP_034190768.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334877.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="peptidoglycan-recognition protein LC isoform X2"
                     /calculated_mol_wt=47178
     Region          264..403
                     /region_name="PGRP"
                     /note="Animal peptidoglycan recognition proteins
                     homologous to Bacteriophage T3 lysozyme; smart00701"
                     /db_xref="CDD:128941"
     Site            order(291,326,400,406,408)
                     /site_type="active"
                     /note="amidase catalytic site [active]"
                     /db_xref="CDD:133475"
     Site            order(291,400,408)
                     /site_type="other"
                     /note="Zn binding residues [ion binding]"
                     /db_xref="CDD:133475"
     Site            order(292..293,322,326,340,347..348,353,400,404,406..408)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:133475"
     CDS             1..427
                     /gene="LOC117609031"
                     /coded_by="XM_034334877.2:1071..2354"
                     /db_xref="GeneID:117609031"
ORIGIN      
        1 mgmvtlvqhh qetapdedry qkdqdqvini ptenrdsddc reeiahplkd tavvvagngs
       61 lagsstqcsd veeeddedet dsderewipd lpvptvyqqh qqvstadgnv vlpnaeasnf
      121 gdvrvknssn vhlgnktfyk gpvtikqfvy tnpvsvqeng nvkddneqth dangsassaa
      181 nnkedstvns svlsqsrnid kvtkwlwtwr caalmctlsl ilvttviavs vhytsnstap
      241 papifpeipd sslggvkvkn vrfversewg aqppsvqltk mklpvpyvii shtatefctt
      301 qsectfhvrf aqtfhiesrr wsdigynflv ggdgyvyvgr swdymgshaf gfnnisigis
      361 figtfnsvip pknqlyaaqr vielgvehgk ispdykllgh rqvsrtlspg dalysiiqtw
      421 phwsstp
//
LOCUS       XP_034190779             319 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein twz isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190779
VERSION     XP_034190779.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334888.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190779.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..319
                     /product="BTB/POZ domain-containing protein twz isoform
                     X1"
                     /calculated_mol_wt=34830
     Region          64..157
                     /region_name="BTB_POZ_KCTD1-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in potassium
                     channel tetramerization domain-containing proteins, KCTD1
                     and KCTD15; cd18361"
                     /db_xref="CDD:349670"
     Site            order(65,67,69..70,72..76,79,99,101,104,106,108,110..112,
                     114,117,121,124..125,127,129,131..133,135..136,139,142,
                     145)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349670"
     Region          <229..314
                     /region_name="H1_KCTD12-like"
                     /note="H1 domain found in potassium channel
                     tetramerization domain-containing proteins; cl41727"
                     /db_xref="CDD:425358"
     Site            order(248,253..254,257,260,263,269,275,280)
                     /site_type="other"
                     /note="G protein beta subunit interface [polypeptide
                     binding]"
                     /db_xref="CDD:409026"
     CDS             1..319
                     /gene="twz"
                     /coded_by="XM_034334888.2:285..1244"
                     /db_xref="GeneID:117609046"
ORIGIN      
        1 mfpsaqikms ifvrllspss spatsptmsn sssptpptpa apaynqkmtg ipcvaaasry
       61 tapvhidvgg tiytssletl tkypesrlak lfngsipivl dslkqhyfid rdggmfrhil
      121 nfmrnsrlli pdnfadldll leearyfdia pmcrqleqmk kerikngstt tttmssssps
      181 ppptnqqgar gtsctsvpcn rdsekirgne tncsyecval hvspdlgerv mlsggralld
      241 evfpettqav idarsgvawh qqdtrhvirf plngycklns vqaitrllna gfrvvasngg
      301 gvegqqfsey lfvrqavnt
//
LOCUS       XP_034190786             315 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein twz isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190786
VERSION     XP_034190786.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334895.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190786.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="BTB/POZ domain-containing protein twz isoform
                     X2"
                     /calculated_mol_wt=34383
     Region          60..153
                     /region_name="BTB_POZ_KCTD1-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in potassium
                     channel tetramerization domain-containing proteins, KCTD1
                     and KCTD15; cd18361"
                     /db_xref="CDD:349670"
     Site            order(61,63,65..66,68..72,75,95,97,100,102,104,106..108,
                     110,113,117,120..121,123,125,127..129,131..132,135,138,
                     141)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349670"
     Region          <225..310
                     /region_name="H1_KCTD12-like"
                     /note="H1 domain found in potassium channel
                     tetramerization domain-containing proteins; cl41727"
                     /db_xref="CDD:425358"
     Site            order(244,249..250,253,256,259,265,271,276)
                     /site_type="other"
                     /note="G protein beta subunit interface [polypeptide
                     binding]"
                     /db_xref="CDD:409026"
     CDS             1..315
                     /gene="twz"
                     /coded_by="XM_034334895.2:283..1230"
                     /db_xref="GeneID:117609046"
ORIGIN      
        1 mfpsaqikmr llspssspat sptmsnsssp tpptpaapay nqkmtgipcv aaasrytapv
       61 hidvggtiyt ssletltkyp esrlaklfng sipivldslk qhyfidrdgg mfrhilnfmr
      121 nsrllipdnf adldllleea ryfdiapmcr qleqmkkeri kngstttttm sssspspppt
      181 nqqgargtsc tsvpcnrdse kirgnetncs yecvalhvsp dlgervmlsg gralldevfp
      241 ettqavidar sgvawhqqdt rhvirfplng ycklnsvqai trllnagfrv vasngggveg
      301 qqfseylfvr qavnt
//
LOCUS       XP_034181831             326 aa            linear   INV 03-SEP-2025
DEFINITION  protein couch potato isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034181831
VERSION     XP_034181831.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325940.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="protein couch potato isoform X1"
                     /calculated_mol_wt=34903
     Region          22..105
                     /region_name="RRM_cpo"
                     /note="RNA recognition motif (RRM) found in Drosophila
                     couch potato (cpo) coding RNA-binding protein and similar
                     proteins; cd12684"
                     /db_xref="CDD:410085"
     Region          224..302
                     /region_name="RRM_scw1_like"
                     /note="RNA recognition motif (RRM) found in yeast cell
                     wall integrity protein scw1 and similar proteins; cd12245"
                     /db_xref="CDD:409691"
     CDS             1..326
                     /gene="LOC117605079"
                     /coded_by="XM_034325940.2:1219..2199"
                     /db_xref="GeneID:117605079"
ORIGIN      
        1 mdaplsqsmd svntvatged evrtlfvsgl pmdakprely llfrayegye gsllkvtskn
       61 gktaspvgfv tfhtragaea akqdlqqgvr fdpdmpqtir lefaksntkv skpkqpaaaa
      121 atshpalmhp ltghlgspff pggpelwhhp laystagelp gtlqhatlvh palhpqvpap
      181 mslphpttlt sihaslphfl pspalaspvg spssqpniav snapcstlfv anlgqfvseh
      241 elkdifssfp gfsrlrmhtk ggspvafvey qdvryaaqam atlqgsflls sdrgairiey
      301 akskmaevgf tnlwiegskr eengqp
//
LOCUS       XP_034181841             325 aa            linear   INV 03-SEP-2025
DEFINITION  protein couch potato isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034181841
VERSION     XP_034181841.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325950.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..325
                     /product="protein couch potato isoform X2"
                     /calculated_mol_wt=34775
     Region          22..104
                     /region_name="RRM_cpo"
                     /note="RNA recognition motif (RRM) found in Drosophila
                     couch potato (cpo) coding RNA-binding protein and similar
                     proteins; cd12684"
                     /db_xref="CDD:410085"
     Region          223..301
                     /region_name="RRM_scw1_like"
                     /note="RNA recognition motif (RRM) found in yeast cell
                     wall integrity protein scw1 and similar proteins; cd12245"
                     /db_xref="CDD:409691"
     CDS             1..325
                     /gene="LOC117605079"
                     /coded_by="XM_034325950.2:1224..2201"
                     /db_xref="GeneID:117605079"
ORIGIN      
        1 mdaplsqsmd svntvatged evrtlfvsgl pmdakprely llfrayegye gsllkvtskn
       61 gktaspvgfv tfhtragaea akqdlqgvrf dpdmpqtirl efaksntkvs kpkqpaaaaa
      121 tshpalmhpl tghlgspffp ggpelwhhpl aystagelpg tlqhatlvhp alhpqvpapm
      181 slphpttlts ihaslphflp spalaspvgs pssqpniavs napcstlfva nlgqfvsehe
      241 lkdifssfpg fsrlrmhtkg gspvafveyq dvryaaqama tlqgsfllss drgairieya
      301 kskmaevgft nlwiegskre engqp
//
LOCUS       XP_034181849             295 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605079 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034181849
VERSION     XP_034181849.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325958.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="uncharacterized protein LOC117605079 isoform X3"
                     /calculated_mol_wt=31653
     Region          1..74
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..271
                     /region_name="RRM_scw1_like"
                     /note="RNA recognition motif (RRM) found in yeast cell
                     wall integrity protein scw1 and similar proteins; cd12245"
                     /db_xref="CDD:409691"
     CDS             1..295
                     /gene="LOC117605079"
                     /coded_by="XM_034325958.2:870..1757"
                     /db_xref="GeneID:117605079"
ORIGIN      
        1 mdakprelyl lfrayegyeg sllkvtskng ktaspvgfvt fhtragaeaa kqdlqqgvrf
       61 dpdmpqtirl efaksntkvs kpkqpaaaaa tshpalmhpl tghlgspffp ggpelwhhpl
      121 aystagelpg tlqhatlvhp alhpqvpapm slphpttlts ihaslphflp spalaspvgs
      181 pssqpniavs napcstlfva nlgqfvsehe lkdifssfpg fsrlrmhtkg gspvafveyq
      241 dvryaaqama tlqgsfllss drgairieya kskmaevgft nlwiegskre engqp
//
LOCUS       XP_076546500             272 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605079 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546500
VERSION     XP_076546500.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690385.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="uncharacterized protein LOC117605079 isoform X4"
                     /calculated_mol_wt=29157
     Region          170..248
                     /region_name="RRM_scw1_like"
                     /note="RNA recognition motif (RRM) found in yeast cell
                     wall integrity protein scw1 and similar proteins; cd12245"
                     /db_xref="CDD:409691"
     CDS             1..272
                     /gene="LOC117605079"
                     /coded_by="XM_076690385.1:852..1670"
                     /db_xref="GeneID:117605079"
ORIGIN      
        1 mgkaygyevf rveqgthlvr erladgrqae gavspvqsir glrgvsaesh kqkwensvag
       61 gvrdfshksr crggetgptd lgspffpggp elwhhplays tagelpgtlq hatlvhpalh
      121 pqvpapmslp hpttltsiha slphflpspa laspvgspss qpniavsnap cstlfvanlg
      181 qfvsehelkd ifssfpgfsr lrmhtkggsp vafveyqdvr yaaqamatlq gsfllssdrg
      241 airieyaksk maevgftnlw iegskreeng qp
//
LOCUS       XP_034181857             265 aa            linear   INV 03-SEP-2025
DEFINITION  protein couch potato isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034181857
VERSION     XP_034181857.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034325966.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..265
                     /product="protein couch potato isoform X5"
                     /calculated_mol_wt=28245
     Region          22..105
                     /region_name="RRM_cpo"
                     /note="RNA recognition motif (RRM) found in Drosophila
                     couch potato (cpo) coding RNA-binding protein and similar
                     proteins; cd12684"
                     /db_xref="CDD:410085"
     Region          <25..247
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Region          224..>248
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..265
                     /gene="LOC117605079"
                     /coded_by="XM_034325966.2:1194..1991"
                     /db_xref="GeneID:117605079"
ORIGIN      
        1 mdaplsqsmd svntvatged evrtlfvsgl pmdakprely llfrayegye gsllkvtskn
       61 gktaspvgfv tfhtragaea akqdlqqgvr fdpdmpqtir lefaksntkv skpkqpaaaa
      121 atshpalmhp ltghlgspff pggpelwhhp laystagelp gtlqhatlvh palhpqvpap
      181 mslphpttlt sihaslphfl pspalaspvg spssqpniav snapcstlfv anlgqfvseh
      241 elkdifsrdq rekktgnlki dingm
//
LOCUS       XP_034175084            1608 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600005 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034175084
VERSION     XP_034175084.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034319193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034175084.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1608
                     /product="uncharacterized protein LOC117600005 isoform X1"
                     /calculated_mol_wt=182977
     Region          186..464
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(188..191,194,196,216,218,248,264..267,317,321..322,
                     324,334..335)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1608
                     /gene="LOC117600005"
                     /coded_by="XM_034319193.2:562..5388"
                     /db_xref="GeneID:117600005"
ORIGIN      
        1 mlniilcwic wlfwaglptl taypvsssik tdlstiyass kwerywiqyt lgilsvtata
       61 ftlvgclccr rprrpkgfqd kaekcnqefk dgnnigqesv fessvdglel dvprmlavad
      121 rvqfeplpve himsgtknss kpkplplsts ffekhdshsn alpehchewf nteelcvpre
      181 klkylreigh gwfgkvvegr adleghrrts knggvvvril teeattkeka wflgeatpyl
      241 klhhqniltl lgfcletdpy lllfescpag dlkgfllsnr dkksqealvk enipirlamd
      301 iltglkhmhs hgfvhtdlsa rnclvasdls aklgdygtgv ekypedyyvv gdralpirws
      361 apesvectdt tietreitfq anmwsyaifl weivtwgnrp yhnksdeqvi qmllslrtdl
      421 lsngiqvlqt ylencpsniy qtihlclnln pqkrpnldev krfllnehte yldfeqrwes
      481 lrinttkhvr saslqdlrgs idsdywttiv ddtprqssfr lgpgelvknv pnvknivqhe
      541 sgseteeesw kgrieqgvyt ekvkqksksv tdlmvlvhid fdsdaeismg tqvsekhgkk
      601 rlpatgsdsd lrhsartdef dealrklrdp fpnnankikt ldnserpkll tltmdhgqtp
      661 ilrlslddke sttsidapsv asvntetrnd khvlrllskg epnlpllryv pddrsfeelk
      721 qnnesecnnv pkhennnntc fsdnfsvnfe snesnlvdvv lwnhaldsal ekkvpgflne
      781 gefnecpess skkqnngtpe lmvtsvstpd tseftkctsy nvndvdverk clpndaeeer
      841 kqlstpddeq ssdsgfrdke sceeeenvcp svpstsadsv vnvptcteee qlqilfeldt
      901 ildaeyystl qgsertiees lssinhveds kiirtneeks etennecssf nvdlegines
      961 veednskpvv qlmqsnsplk tnyfnqeleh edendnecen rnesecenen eseyenenes
     1021 kserihekes iheddedsst mslrsdnsyv sfeieeefvt airnelrekl pcaqmsvvep
     1081 lethdddhpm sndienkwdd dddnedssnq gsggvgisir ynvygtplsp iqeerestlt
     1141 seslisnsrd vsiaskesvg sddillidtr tnkmilfdgs deklqdddrd ttnedlsdde
     1201 nldyncsivr srddkshimi psggaplpsp eeeskwqqsf plplplpled nlmstsfgte
     1261 hewgsqddde eeeeegedns sssgefiwkr ysephieqvr kslsdneeav vndieeemde
     1321 evekegkkre adaeeeeeee eeeftpsawn atlaphrsal rspdktlktn dqkksvwfkk
     1381 qryhcvyeyp ketlsaenqg ettttwepts ysdweemide prldlypldy ddvnqtgnee
     1441 ffvsssnrpf qfqasdskyv sqffpgasts mseeqdevdg nrleaqenkl ellndcnygq
     1501 qhqlgelrht rnrlklnlst ngapfvtikq lkegdteqlg dkgmeldsts qtinfthdqh
     1561 ilsnslnhnt lpmvppddlq qeksfdpitg ksaecdsqek ieptstvf
//
LOCUS       XP_034183491            1600 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117600005 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183491
VERSION     XP_034183491.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327600.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183491.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1600
                     /product="uncharacterized protein LOC117600005 isoform X2"
                     /calculated_mol_wt=182060
     Region          178..456
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(180..183,186,188,208,210,240,256..259,309,313..314,
                     316,326..327)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1600
                     /gene="LOC117600005"
                     /coded_by="XM_034327600.2:561..5363"
                     /db_xref="GeneID:117600005"
ORIGIN      
        1 mlniilcwic wlfwaglptl taypvsssik tdlstiyass kwerywiqyt lgilsvtata
       61 ftlvgclccr rprrpkgfqe fkdgnnigqe svfessvdgl eldvprmlav adrvqfeplp
      121 vehimsgtkn sskpkplpls tsffekhdsh snalpehche wfnteelcvp reklkylrei
      181 ghgwfgkvve gradleghrr tsknggvvvr ilteeattke kawflgeatp ylklhhqnil
      241 tllgfcletd pylllfescp agdlkgflls nrdkksqeal vkenipirla mdiltglkhm
      301 hshgfvhtdl sarnclvasd lsaklgdygt gvekypedyy vvgdralpir wsapesvect
      361 dttietreit fqanmwsyai flweivtwgn rpyhnksdeq viqmllslrt dllsngiqvl
      421 qtylencpsn iyqtihlcln lnpqkrpnld evkrfllneh teyldfeqrw eslrinttkh
      481 vrsaslqdlr gsidsdywtt ivddtprqss frlgpgelvk nvpnvknivq hesgseteee
      541 swkgrieqgv ytekvkqksk svtdlmvlvh idfdsdaeis mgtqvsekhg kkrlpatgsd
      601 sdlrhsartd efdealrklr dpfpnnanki ktldnserpk lltltmdhgq tpilrlsldd
      661 kesttsidap svasvntetr ndkhvlrlls kgepnlpllr yvpddrsfee lkqnnesecn
      721 nvpkhennnn tcfsdnfsvn fesnesnlvd vvlwnhalds alekkvpgfl negefnecpe
      781 ssskkqnngt pelmvtsvst pdtseftkct synvndvdve rkclpndaee erkqlstpdd
      841 eqssdsgfrd kesceeeenv cpsvpstsad svvnvptcte eeqlqilfel dtildaeyys
      901 tlqgsertie eslssinhve dskiirtnee ksetennecs sfnvdlegin esveednskp
      961 vvqlmqsnsp lktnyfnqel ehedendnec enrnesecen eneseyenen eskserihek
     1021 esiheddeds stmslrsdns yvsfeieeef vtairnelre klpcaqmsvv eplethdddh
     1081 pmsndienkw dddddnedss nqgsggvgis irynvygtpl spiqeerest ltseslisns
     1141 rdvsiaskes vgsddillid trtnkmilfd gsdeklqddd rdttnedlsd denldyncsi
     1201 vrsrddkshi mipsggaplp speeeskwqq sfplplplpl ednlmstsfg tehewgsqdd
     1261 deeeeeeged nssssgefiw krysephieq vrkslsdnee avvndieeem deevekegkk
     1321 readaeeeee eeeeeftpsa wnatlaphrs alrspdktlk tndqkksvwf kkqryhcvye
     1381 ypketlsaen qgettttwep tsysdweemi deprldlypl dyddvnqtgn eeffvsssnr
     1441 pfqfqasdsk yvsqffpgas tsmseeqdev dgnrleaqen klellndcny gqqhqlgelr
     1501 htrnrlklnl stngapfvti kqlkegdteq lgdkgmelds tsqtinfthd qhilsnslnh
     1561 ntlpmvppdd lqqeksfdpi tgksaecdsq ekieptstvf
//
LOCUS       XP_076546501            1494 aa            linear   INV 03-SEP-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546501
VERSION     XP_076546501.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690386.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1494
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X1"
                     /calculated_mol_wt=163567
     Region          25..122
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <227..423
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          232..253
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          254..282
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          284..324
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          327..358
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          359..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          386..412
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          413..444
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          445..475
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <455..>649
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          476..502
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          530..559
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          565..591
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          592..611
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          798..1121
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     Region          <1083..1394
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1494
                     /gene="LRR"
                     /coded_by="XM_076690386.1:107..4591"
                     /db_xref="GeneID:117609622"
ORIGIN      
        1 mstrsqltkd lnesvkallg khvkillknv vkletkqdkq enrvlvfspc rlflltakvp
       61 tridchfhyl eitsveskra nqlslsvger yynftttgag adttevdami ealhtairni
      121 fptvplnyii rkievipasr lqsirgsela rsteatrhtg pcggfstqya cmcdlhgvpy
      181 reevawdvdt iylshdtrel nlrdfdhldq kdlvpiisal eyntwftklr ashlklshep
      241 lerllhvmrr slsiqelyld nlgikwdfah klslalisna ntilqtidls hntiedkgas
      301 slcgiiaklm qggahlsgpi gklpkglqkl nlahcgltgk gisqiahals lnrsmptslq
      361 ylnlsenslk ddinnlcnfl aqpnslthld lsgtdttlec lfgallrgca tnlvhlnvar
      421 nsfsskktke ippsfkqfft atlslkylni sscklpleal khlllglacn estvgleldm
      481 sgnnlgsmga hvlescihgv rciasldisd snmdvdlaqv itaigknksi kqlymgrntv
      541 smkskhiavv mdalvqmlqe ddcvlqalhl pdsrlksdly nlinalgsnt clhtldisgn
      601 qigdpgarll akalqinnhl rtiiydknni tlqgyadivh alekncsvrh mpfpiydlqp
      661 cmktsaekte qlakkiqdll qrnvtpckys hgqafrlqqg fllsstqqmv drlvvqtqdt
      721 ikaiaaescd anndinyatg liqdadnskq llprlhevlq rrdesnpies klhemanelh
      781 kivtlylkds ldamikcase qcptiisqtv irgdesesia ieddlrmsck eknqissefi
      841 hatiteqaga divnrvneln lavaahvsdr itdeviesls rsyknligdc dnrtrsstpd
      901 vlrpsagsms sgsvigvtst vtstalpvgr tsiaseedcq qepyslvnsi gqcsndqspm
      961 kldylnlatp hlsnkrkslh grklrpksvv dsveglsadd ipdllpslpk sqaeaisete
     1021 hsltesldsv selpntvgqq lqhlvksrpr rtktraptrp mlrsdqpidg lalgegldvf
     1081 frpttpttpl isptsddssl htfptdgspn lsltshkstp pdldkkpgcn spmlktllep
     1141 tprsrssdnl ekfsplvgrr sqgdspltas plarrnttdn aqnheriask sesnkdtcsn
     1201 icnnsisnsa dvskrstlpi igsgtssrns rdsdensklt sgnssdkdvt slprdhenrr
     1261 slpkkstmdi dktvnqplps lklrstgfdl rsptngsntk nnsdsskspv lkpltkgnss
     1321 vsdgknngai lktkpappat apkprpwsma tdrksgefnl lsdgsspnts agntpdsgda
     1381 ldestdsgvs gpaslpptls asstasslsn tsvekrsvre laaslnkskp ekkenehtap
     1441 aawrsvlqrs irqpdvkvte vpktveedhi nfklrrtsfl rdsnfnynnd vvdv
//
LOCUS       XP_034195386            1487 aa            linear   INV 03-SEP-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195386
VERSION     XP_034195386.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339495.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1487
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X2"
                     /calculated_mol_wt=162707
     Region          25..122
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <227..423
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          232..253
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          254..282
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          284..324
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          327..358
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          359..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          386..412
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          413..444
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          445..475
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <455..>649
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          476..502
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          530..559
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          565..591
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          592..611
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          798..1114
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     Region          <1076..1387
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1487
                     /gene="LRR"
                     /coded_by="XM_034339495.2:107..4570"
                     /db_xref="GeneID:117609622"
ORIGIN      
        1 mstrsqltkd lnesvkallg khvkillknv vkletkqdkq enrvlvfspc rlflltakvp
       61 tridchfhyl eitsveskra nqlslsvger yynftttgag adttevdami ealhtairni
      121 fptvplnyii rkievipasr lqsirgsela rsteatrhtg pcggfstqya cmcdlhgvpy
      181 reevawdvdt iylshdtrel nlrdfdhldq kdlvpiisal eyntwftklr ashlklshep
      241 lerllhvmrr slsiqelyld nlgikwdfah klslalisna ntilqtidls hntiedkgas
      301 slcgiiaklm qggahlsgpi gklpkglqkl nlahcgltgk gisqiahals lnrsmptslq
      361 ylnlsenslk ddinnlcnfl aqpnslthld lsgtdttlec lfgallrgca tnlvhlnvar
      421 nsfsskktke ippsfkqfft atlslkylni sscklpleal khlllglacn estvgleldm
      481 sgnnlgsmga hvlescihgv rciasldisd snmdvdlaqv itaigknksi kqlymgrntv
      541 smkskhiavv mdalvqmlqe ddcvlqalhl pdsrlksdly nlinalgsnt clhtldisgn
      601 qigdpgarll akalqinnhl rtiiydknni tlqgyadivh alekncsvrh mpfpiydlqp
      661 cmktsaekte qlakkiqdll qrnvtpckys hgqafrlqqg fllsstqqmv drlvvqtqdt
      721 ikaiaaescd anndinyatg liqdadnskq llprlhevlq rrdesnpies klhemanelh
      781 kivtlylkds ldamikcase qcptiisqtv irgdesesia ieddlrmsck eknqissefi
      841 hatiteqaga divnrvneln lavaahvsdr itdeviesls rsyknligdc dnrtrsstpd
      901 vlrpsagsms sgsvigvtst vtstalpvgr tsiaseedcq qepyslvnsi gqcsndqspm
      961 atphlsnkrk slhgrklrpk svvdsvegls addipdllps lpksqaeais etehsltesl
     1021 dsvselpntv gqqlqhlvks rprrtktrap trpmlrsdqp idglalgegl dvffrpttpt
     1081 tplisptsdd sslhtfptdg spnlsltshk stppdldkkp gcnspmlktl leptprsrss
     1141 dnlekfsplv grrsqgdspl tasplarrnt tdnaqnheri asksesnkdt csnicnnsis
     1201 nsadvskrst lpiigsgtss rnsrdsdens kltsgnssdk dvtslprdhe nrrslpkkst
     1261 mdidktvnqp lpslklrstg fdlrsptngs ntknnsdssk spvlkpltkg nssvsdgknn
     1321 gailktkpap patapkprpw smatdrksge fnllsdgssp ntsagntpds gdaldestds
     1381 gvsgpaslpp tlsasstass lsntsvekrs vrelaaslnk skpekkeneh tapaawrsvl
     1441 qrsirqpdvk vtevpktvee dhinfklrrt sflrdsnfny nndvvdv
//
LOCUS       XP_076546502            1480 aa            linear   INV 03-SEP-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546502
VERSION     XP_076546502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690387.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1480
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X3"
                     /calculated_mol_wt=162193
     Region          25..122
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          229..488
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          284..312
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          313..344
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          345..371
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          372..398
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          399..430
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          431..461
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <441..>635
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          462..515
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          516..545
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          551..577
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          578..597
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          784..1107
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     Region          <1069..1380
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1480
                     /gene="LRR"
                     /coded_by="XM_076690387.1:107..4549"
                     /db_xref="GeneID:117609622"
ORIGIN      
        1 mstrsqltkd lnesvkallg khvkillknv vkletkqdkq enrvlvfspc rlflltakvp
       61 tridchfhyl eitsveskra nqlslsvger yynftttgag adttevdami ealhtairni
      121 fptvplnyii rkievipasr lqsirgsela rsteatrhtg pcggfstqya cmcdlhgvpy
      181 reevawdvdt iylshdtrel nlrdfdhldq kdlvpiisal eyntwftklr ashlklshep
      241 lerllhvmrr slsiqelyld nlgikwdfah klslalisna ntilqtidls hntiedkgga
      301 hlsgpigklp kglqklnlah cgltgkgisq iahalslnrs mptslqylnl senslkddin
      361 nlcnflaqpn slthldlsgt dttleclfga llrgcatnlv hlnvarnsfs skktkeipps
      421 fkqfftatls lkylnissck lplealkhll lglacnestv gleldmsgnn lgsmgahvle
      481 scihgvrcia sldisdsnmd vdlaqvitai gknksikqly mgrntvsmks khiavvmdal
      541 vqmlqeddcv lqalhlpdsr lksdlynlin algsntclht ldisgnqigd pgarllakal
      601 qinnhlrtii ydknnitlqg yadivhalek ncsvrhmpfp iydlqpcmkt saekteqlak
      661 kiqdllqrnv tpckyshgqa frlqqgflls stqqmvdrlv vqtqdtikai aaescdannd
      721 inyatgliqd adnskqllpr lhevlqrrde snpiesklhe manelhkivt lylkdsldam
      781 ikcaseqcpt iisqtvirgd esesiaiedd lrmsckeknq issefihati teqagadivn
      841 rvnelnlava ahvsdritde vieslsrsyk nligdcdnrt rsstpdvlrp sagsmssgsv
      901 igvtstvtst alpvgrtsia seedcqqepy slvnsigqcs ndqspmkldy lnlatphlsn
      961 krkslhgrkl rpksvvdsve glsaddipdl lpslpksqae aisetehslt esldsvselp
     1021 ntvgqqlqhl vksrprrtkt raptrpmlrs dqpidglalg egldvffrpt tpttplispt
     1081 sddsslhtfp tdgspnlslt shkstppdld kkpgcnspml ktlleptprs rssdnlekfs
     1141 plvgrrsqgd spltasplar rnttdnaqnh eriasksesn kdtcsnicnn sisnsadvsk
     1201 rstlpiigsg tssrnsrdsd enskltsgns sdkdvtslpr dhenrrslpk kstmdidktv
     1261 nqplpslklr stgfdlrspt ngsntknnsd sskspvlkpl tkgnssvsdg knngailktk
     1321 pappatapkp rpwsmatdrk sgefnllsdg sspntsagnt pdsgdaldes tdsgvsgpas
     1381 lpptlsasst asslsntsve krsvrelaas lnkskpekke nehtapaawr svlqrsirqp
     1441 dvkvtevpkt veedhinfkl rrtsflrdsn fnynndvvdv
//
LOCUS       XP_076546503            1443 aa            linear   INV 03-SEP-2025
DEFINITION  capping protein regulator and myosin 1 linker 1 leucine rich repeat
            protein isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546503
VERSION     XP_076546503.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690388.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1443
                     /product="capping protein regulator and myosin 1 linker 1
                     leucine rich repeat protein isoform X4"
                     /calculated_mol_wt=157332
     Region          25..122
                     /region_name="Carm_PH"
                     /note="Carmil pleckstrin homology domain; pfam17888"
                     /db_xref="CDD:436119"
     Region          <227..423
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          232..253
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          254..282
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          284..324
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          327..358
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          359..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          386..412
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          413..444
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          445..475
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <455..>649
                     /region_name="RNA1"
                     /note="Ran GTPase-activating protein (RanGAP) involved in
                     mRNA processing and transport [Translation, ribosomal
                     structure and biogenesis]; COG5238"
                     /db_xref="CDD:444072"
     Region          476..502
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          530..559
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          565..591
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          592..611
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          798..1121
                     /region_name="CARMIL_C"
                     /note="CARMIL C-terminus; pfam16000"
                     /db_xref="CDD:464966"
     Region          <1083..1394
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1443
                     /gene="LRR"
                     /coded_by="XM_076690388.1:107..4438"
                     /db_xref="GeneID:117609622"
ORIGIN      
        1 mstrsqltkd lnesvkallg khvkillknv vkletkqdkq enrvlvfspc rlflltakvp
       61 tridchfhyl eitsveskra nqlslsvger yynftttgag adttevdami ealhtairni
      121 fptvplnyii rkievipasr lqsirgsela rsteatrhtg pcggfstqya cmcdlhgvpy
      181 reevawdvdt iylshdtrel nlrdfdhldq kdlvpiisal eyntwftklr ashlklshep
      241 lerllhvmrr slsiqelyld nlgikwdfah klslalisna ntilqtidls hntiedkgas
      301 slcgiiaklm qggahlsgpi gklpkglqkl nlahcgltgk gisqiahals lnrsmptslq
      361 ylnlsenslk ddinnlcnfl aqpnslthld lsgtdttlec lfgallrgca tnlvhlnvar
      421 nsfsskktke ippsfkqfft atlslkylni sscklpleal khlllglacn estvgleldm
      481 sgnnlgsmga hvlescihgv rciasldisd snmdvdlaqv itaigknksi kqlymgrntv
      541 smkskhiavv mdalvqmlqe ddcvlqalhl pdsrlksdly nlinalgsnt clhtldisgn
      601 qigdpgarll akalqinnhl rtiiydknni tlqgyadivh alekncsvrh mpfpiydlqp
      661 cmktsaekte qlakkiqdll qrnvtpckys hgqafrlqqg fllsstqqmv drlvvqtqdt
      721 ikaiaaescd anndinyatg liqdadnskq llprlhevlq rrdesnpies klhemanelh
      781 kivtlylkds ldamikcase qcptiisqtv irgdesesia ieddlrmsck eknqissefi
      841 hatiteqaga divnrvneln lavaahvsdr itdeviesls rsyknligdc dnrtrsstpd
      901 vlrpsagsms sgsvigvtst vtstalpvgr tsiaseedcq qepyslvnsi gqcsndqspm
      961 kldylnlatp hlsnkrkslh grklrpksvv dsveglsadd ipdllpslpk sqaeaisete
     1021 hsltesldsv selpntvgqq lqhlvksrpr rtktraptrp mlrsdqpidg lalgegldvf
     1081 frpttpttpl isptsddssl htfptdgspn lsltshkstp pdldkkpgcn spmlktllep
     1141 tprsrssdnl ekfsplvgrr sqgdspltas plarrnttdn aqnheriask sesnkdtcsn
     1201 icnnsisnsa dvskrstlpi igsgtssrns rdsdensklt sgnssdkdvt slprdhenrr
     1261 slpkkstmdi dktvnqplps lklrstgfdl rsptngsntk nnsdsskspv lkpltkgnss
     1321 vsdgknngai lktkpappat apkprpwsma tdrksgefnl lsdgsspnts agntpdsgda
     1381 ldestdsgvs gpaslpptls asstasslsn tsvekrsvre laaslnkskp ekkengngsa
     1441 eng
//
LOCUS       XP_034172526             553 aa            linear   INV 03-SEP-2025
DEFINITION  dynein regulatory complex subunit 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034172526
VERSION     XP_034172526.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034316635.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034172526.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..553
                     /product="dynein regulatory complex subunit 3 isoform X1"
                     /calculated_mol_wt=64702
     Region          60..205
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          73..94
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          95..116
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          117..138
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          139..150
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..553
                     /gene="LOC117600593"
                     /coded_by="XM_034316635.2:71..1732"
                     /db_xref="GeneID:117600593"
ORIGIN      
        1 midqdvptil qefvqprvin qdmlmdlvid qgpkgeagkl fyedgiklne vteiriefln
       61 ilkidnlwit pnlvklklsn niiekienld vlinlkeldl sfnhiriien lhnlikleil
      121 llfnneisnv egidnlcnlt ifsignnait dwkhvlylrt fkklrslnmn gnpctekddy
      181 leyviafipq liyyqykmit deqrevaaek hyrvlrtlqe temkedeelq aqeeyekrla
      241 yhtnafveyl dsdelfhqmf indkegkels mvnedtqnah eeykanfvai sqelcelglk
      301 eqdernnein hftscvnkgk tisqnqgrii vnevlekktk ilsdvkqllk riagevdtat
      361 leevtqkaqq fseefneivi dawkklmsie velheqiedi nemfrinmtd mmdsfltiar
      421 gyfsqlrnse aeysdtingv ilyylsgled dtkvpshlln lcedkdtlnq nltnshekhl
      481 qvldarednm lkrlknwlee ytenlvkyen ernnqqilei shfadsqqqe fvqllqqlnl
      541 nvddsevila les
//
LOCUS       XP_034173402             449 aa            linear   INV 03-SEP-2025
DEFINITION  dynein regulatory complex subunit 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034173402
VERSION     XP_034173402.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034317511.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034173402.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="dynein regulatory complex subunit 3 isoform X2"
                     /calculated_mol_wt=52607
     Region          <47..106
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     CDS             1..449
                     /gene="LOC117600593"
                     /coded_by="XM_034317511.2:71..1420"
                     /db_xref="GeneID:117600593"
ORIGIN      
        1 midqdvptil qefvqprvin qdmlmdlvid qgpkgeagkl fyedgiklne vlylrtfkkl
       61 rslnmngnpc tekddyleyv iafipqliyy qykmitdeqr evaaekhyrv lrtlqetemk
      121 edeelqaqee yekrlayhtn afveyldsde lfhqmfindk egkelsmvne dtqnaheeyk
      181 anfvaisqel celglkeqde rnneinhfts cvnkgktisq nqgriivnev lekktkilsd
      241 vkqllkriag evdtatleev tqkaqqfsee fneividawk klmsievelh eqiedinemf
      301 rinmtdmmds fltiargyfs qlrnseaeys dtingvilyy lsgleddtkv pshllnlced
      361 kdtlnqnltn shekhlqvld arednmlkrl knwleeyten lvkyenernn qqileishfa
      421 dsqqqefvql lqqlnlnvdd sevilales
//
LOCUS       XP_034175936             278 aa            linear   INV 03-SEP-2025
DEFINITION  enoyl-CoA delta isomerase 1, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034175936
VERSION     XP_034175936.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320045.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="enoyl-CoA delta isomerase 1, mitochondrial"
                     /calculated_mol_wt=30848
     Region          33..278
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(46,48,80,84..88,127,129..131,154..155,158)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(86,131)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(111,119,140..143,156..159,165,167..169,171..172,
                     177..178,180..181,183..184,187,198,201)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..278
                     /gene="LOC117602083"
                     /coded_by="XM_034320045.2:166..1002"
                     /db_xref="GeneID:117602083"
ORIGIN      
        1 mfavrriskg ikiplhatys tnsklievth dnqtgiatis manppvnsln kdllsalkts
       61 lmdvqqkqck gviltsslpm vfsagldime mynrnekqlt efwqtlqdtw ltlygleipv
      121 aaaingaspa ggcllalste yrvivegkht iglnetqlgi vaptwfrsly ietlgyrtae
      181 iallrgtlfn vkkaleiglv deivpdktsa iskcqdyias fknipskgra ktkldlrqhn
      241 ldwlkenrel dlntfltliq lpkvqaglkl yieslkqk
//
LOCUS       XP_034176791             278 aa            linear   INV 03-SEP-2025
DEFINITION  enoyl-CoA delta isomerase 1, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034176791
VERSION     XP_034176791.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034320900.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="enoyl-CoA delta isomerase 1, mitochondrial"
                     /calculated_mol_wt=30848
     Region          33..278
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(46,48,80,84..88,127,129..131,154..155,158)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(86,131)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(111,119,140..143,156..159,165,167..169,171..172,
                     177..178,180..181,183..184,187,198,201)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..278
                     /gene="LOC117602083"
                     /coded_by="XM_034320900.2:169..1005"
                     /db_xref="GeneID:117602083"
ORIGIN      
        1 mfavrriskg ikiplhatys tnsklievth dnqtgiatis manppvnsln kdllsalkts
       61 lmdvqqkqck gviltsslpm vfsagldime mynrnekqlt efwqtlqdtw ltlygleipv
      121 aaaingaspa ggcllalste yrvivegkht iglnetqlgi vaptwfrsly ietlgyrtae
      181 iallrgtlfn vkkaleiglv deivpdktsa iskcqdyias fknipskgra ktkldlrqhn
      241 ldwlkenrel dlntfltliq lpkvqaglkl yieslkqk
//
LOCUS       XP_034177633             278 aa            linear   INV 03-SEP-2025
DEFINITION  enoyl-CoA delta isomerase 1, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034177633
VERSION     XP_034177633.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034321742.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="enoyl-CoA delta isomerase 1, mitochondrial"
                     /calculated_mol_wt=30848
     Region          33..278
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(46,48,80,84..88,127,129..131,154..155,158)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(86,131)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(111,119,140..143,156..159,165,167..169,171..172,
                     177..178,180..181,183..184,187,198,201)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..278
                     /gene="LOC117602083"
                     /coded_by="XM_034321742.2:159..995"
                     /db_xref="GeneID:117602083"
ORIGIN      
        1 mfavrriskg ikiplhatys tnsklievth dnqtgiatis manppvnsln kdllsalkts
       61 lmdvqqkqck gviltsslpm vfsagldime mynrnekqlt efwqtlqdtw ltlygleipv
      121 aaaingaspa ggcllalste yrvivegkht iglnetqlgi vaptwfrsly ietlgyrtae
      181 iallrgtlfn vkkaleiglv deivpdktsa iskcqdyias fknipskgra ktkldlrqhn
      241 ldwlkenrel dlntfltliq lpkvqaglkl yieslkqk
//
LOCUS       XP_034179294             278 aa            linear   INV 03-SEP-2025
DEFINITION  enoyl-CoA delta isomerase 1, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034179294
VERSION     XP_034179294.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034323403.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="enoyl-CoA delta isomerase 1, mitochondrial"
                     /calculated_mol_wt=30848
     Region          33..278
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(46,48,80,84..88,127,129..131,154..155,158)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(86,131)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(111,119,140..143,156..159,165,167..169,171..172,
                     177..178,180..181,183..184,187,198,201)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..278
                     /gene="LOC117602083"
                     /coded_by="XM_034323403.2:129..965"
                     /db_xref="GeneID:117602083"
ORIGIN      
        1 mfavrriskg ikiplhatys tnsklievth dnqtgiatis manppvnsln kdllsalkts
       61 lmdvqqkqck gviltsslpm vfsagldime mynrnekqlt efwqtlqdtw ltlygleipv
      121 aaaingaspa ggcllalste yrvivegkht iglnetqlgi vaptwfrsly ietlgyrtae
      181 iallrgtlfn vkkaleiglv deivpdktsa iskcqdyias fknipskgra ktkldlrqhn
      241 ldwlkenrel dlntfltliq lpkvqaglkl yieslkqk
//
LOCUS       XP_034178483             278 aa            linear   INV 03-SEP-2025
DEFINITION  enoyl-CoA delta isomerase 1, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034178483
VERSION     XP_034178483.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034322592.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="enoyl-CoA delta isomerase 1, mitochondrial"
                     /calculated_mol_wt=30848
     Region          33..278
                     /region_name="crotonase-like"
                     /note="Crotonase/Enoyl-Coenzyme A (CoA) hydratase
                     superfamily. This superfamily contains a diverse set of
                     enzymes including enoyl-CoA hydratase, napthoate synthase,
                     methylmalonyl-CoA decarboxylase, 3-hydoxybutyryl-CoA
                     dehydratase, and dienoyl-CoA isomerase; cl23717"
                     /db_xref="CDD:474030"
     Site            order(46,48,80,84..88,127,129..131,154..155,158)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:119339"
     Site            order(86,131)
                     /site_type="active"
                     /note="oxyanion hole (OAH) forming residues [active]"
                     /db_xref="CDD:119339"
     Site            order(111,119,140..143,156..159,165,167..169,171..172,
                     177..178,180..181,183..184,187,198,201)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:119339"
     CDS             1..278
                     /gene="LOC117602083"
                     /coded_by="XM_034322592.2:174..1010"
                     /db_xref="GeneID:117602083"
ORIGIN      
        1 mfavrriskg ikiplhatys tnsklievth dnqtgiatis manppvnsln kdllsalkts
       61 lmdvqqkqck gviltsslpm vfsagldime mynrnekqlt efwqtlqdtw ltlygleipv
      121 aaaingaspa ggcllalste yrvivegkht iglnetqlgi vaptwfrsly ietlgyrtae
      181 iallrgtlfn vkkaleiglv deivpdktsa iskcqdyias fknipskgra ktkldlrqhn
      241 ldwlkenrel dlntfltliq lpkvqaglkl yieslkqk
//
LOCUS       XP_034174688             432 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 13 [Osmia lignaria lignaria].
ACCESSION   XP_034174688
VERSION     XP_034174688.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034318797.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..432
                     /product="WD repeat-containing protein 13"
                     /calculated_mol_wt=48201
     Region          <109..426
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          119..158
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          163..203
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          211..247
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          254..293
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          301..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          355..377
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..432
                     /gene="LOC117601516"
                     /coded_by="XM_034318797.2:16..1314"
                     /db_xref="GeneID:117601516"
ORIGIN      
        1 mlsmwhqqvf aldakynaqr vnklptlgml yirrrnqllk ekfskdptir enylklrskl
       61 lflrygenle qnslsshtte eekseikikl hnvqrkstae sfafegvhhv fdqhnapvtm
      121 lkfanndrsk lccasldgsl sicdaigtpp kvialleghk kgvtafdwsi sndlivsssl
      181 dctirlwnvs npetgpnclr tvndqqraev lccgfipinn nlvvagnsqg lvqilnistg
      241 iytrggtcki ggkilsltce gsgglviwag ndrgiimsfq lepgmgrltk lrrvqeiggm
      301 itslswrswl skdnpwpall vssacnvvll yhiidnhgsl slwtkypikh kqrfvrstfc
      361 pqmetcliat gsedgtihll dsaregmaak inrlqghats tlalsfnyde sllasadyqg
      421 liilwrnrer hi
//
LOCUS       XP_034191075            1076 aa            linear   INV 03-SEP-2025
DEFINITION  non-specific lethal 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191075
VERSION     XP_034191075.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335184.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191075.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1076
                     /product="non-specific lethal 1 isoform X1"
                     /calculated_mol_wt=119973
     Region          829..>916
                     /region_name="PEHE"
                     /note="PEHE domain; pfam15275"
                     /db_xref="CDD:464607"
     CDS             1..1076
                     /gene="nsl1"
                     /coded_by="XM_034335184.2:1779..5009"
                     /db_xref="GeneID:117609176"
ORIGIN      
        1 mgvstahvkc rcgcvrtwep ldveaaalav svavmapalt eggpqkpenl lpplpasgfl
       61 speqatevcq nreilakfvv sanvqpskid dqclrgiskd lirdvinsky andldslspf
      121 thgsdlitkk glinqdceep dipgspekka tdiimndpsm gdqadnmgfl kssadlpmvg
      181 setagdnknl dvdqimafgt dittdneqcn mgnvgdigqn veeilqviks iegvenaesi
      241 sagggepvqs tvdgvemfsm peegfssfer dllndvdvms icnniniget vdqqkintlk
      301 lqqdivaqkq yeserrcafl lrrlrklqar ivgrhvaeen tgvlelahhg vkkylfqelv
      361 nissksnvrn fpeissslss flqkiekmcv aqsnsvnrqr lccryfgggs rdslgstsgn
      421 nsnrqpvfgt pqvkikgdev esvagplatq lhivesnlds dctasssgge scdemqsfnn
      481 lhqqhlsisk raawryaqdr agvaarwtwl qaqisdleyr irqhnelqrh vrankglvil
      541 dnaetvngys gvlpgstgry nspegelpas rtrpfvwsfy rkrkllqldr lhefskraak
      601 astvrcncdh vlppcalctg rsdpmqmqep ieqmsvqeri alvdpsfhpv lsfsddvtqg
      661 nhvdaimksv ewqqkmlrgn lritrlkdke tndrrnkklp ghrakyaarl kkssssilta
      721 rikrkmlkgk rnrlghdrsv hglsrkrvqk ltteddddis alsssskhss pvpsplhhta
      781 astekntike kssishgrlr qnsydidniv ipysvaastr leklqykeil tpkwrlceep
      841 lkldikngvm hrpsqdsdfe dmsdetialr hersereenk rfmtymtlph qsrirhnrrt
      901 dsradsgant pdpmsphasd fggdmmspit sppptpqvqd aehqqnldth rssalqnalr
      961 rrttptlrlg kddstlmned eneifpyepr vfplseevyd kmlevmpdgh wqassaslcs
     1021 reeekadddg emdspesdst esaccdiege dpndpewtva ddrdtekeri rptakr
//
LOCUS       XP_034191066            1076 aa            linear   INV 03-SEP-2025
DEFINITION  non-specific lethal 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191066
VERSION     XP_034191066.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335175.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191066.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1076
                     /product="non-specific lethal 1 isoform X1"
                     /calculated_mol_wt=119973
     Region          829..>916
                     /region_name="PEHE"
                     /note="PEHE domain; pfam15275"
                     /db_xref="CDD:464607"
     CDS             1..1076
                     /gene="nsl1"
                     /coded_by="XM_034335175.2:799..4029"
                     /db_xref="GeneID:117609176"
ORIGIN      
        1 mgvstahvkc rcgcvrtwep ldveaaalav svavmapalt eggpqkpenl lpplpasgfl
       61 speqatevcq nreilakfvv sanvqpskid dqclrgiskd lirdvinsky andldslspf
      121 thgsdlitkk glinqdceep dipgspekka tdiimndpsm gdqadnmgfl kssadlpmvg
      181 setagdnknl dvdqimafgt dittdneqcn mgnvgdigqn veeilqviks iegvenaesi
      241 sagggepvqs tvdgvemfsm peegfssfer dllndvdvms icnniniget vdqqkintlk
      301 lqqdivaqkq yeserrcafl lrrlrklqar ivgrhvaeen tgvlelahhg vkkylfqelv
      361 nissksnvrn fpeissslss flqkiekmcv aqsnsvnrqr lccryfgggs rdslgstsgn
      421 nsnrqpvfgt pqvkikgdev esvagplatq lhivesnlds dctasssgge scdemqsfnn
      481 lhqqhlsisk raawryaqdr agvaarwtwl qaqisdleyr irqhnelqrh vrankglvil
      541 dnaetvngys gvlpgstgry nspegelpas rtrpfvwsfy rkrkllqldr lhefskraak
      601 astvrcncdh vlppcalctg rsdpmqmqep ieqmsvqeri alvdpsfhpv lsfsddvtqg
      661 nhvdaimksv ewqqkmlrgn lritrlkdke tndrrnkklp ghrakyaarl kkssssilta
      721 rikrkmlkgk rnrlghdrsv hglsrkrvqk ltteddddis alsssskhss pvpsplhhta
      781 astekntike kssishgrlr qnsydidniv ipysvaastr leklqykeil tpkwrlceep
      841 lkldikngvm hrpsqdsdfe dmsdetialr hersereenk rfmtymtlph qsrirhnrrt
      901 dsradsgant pdpmsphasd fggdmmspit sppptpqvqd aehqqnldth rssalqnalr
      961 rrttptlrlg kddstlmned eneifpyepr vfplseevyd kmlevmpdgh wqassaslcs
     1021 reeekadddg emdspesdst esaccdiege dpndpewtva ddrdtekeri rptakr
//
LOCUS       XP_034191084            1031 aa            linear   INV 03-SEP-2025
DEFINITION  non-specific lethal 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191084
VERSION     XP_034191084.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335193.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191084.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1031
                     /product="non-specific lethal 1 isoform X2"
                     /calculated_mol_wt=114992
     Region          829..>916
                     /region_name="PEHE"
                     /note="PEHE domain; pfam15275"
                     /db_xref="CDD:464607"
     CDS             1..1031
                     /gene="nsl1"
                     /coded_by="XM_034335193.2:1779..4874"
                     /db_xref="GeneID:117609176"
ORIGIN      
        1 mgvstahvkc rcgcvrtwep ldveaaalav svavmapalt eggpqkpenl lpplpasgfl
       61 speqatevcq nreilakfvv sanvqpskid dqclrgiskd lirdvinsky andldslspf
      121 thgsdlitkk glinqdceep dipgspekka tdiimndpsm gdqadnmgfl kssadlpmvg
      181 setagdnknl dvdqimafgt dittdneqcn mgnvgdigqn veeilqviks iegvenaesi
      241 sagggepvqs tvdgvemfsm peegfssfer dllndvdvms icnniniget vdqqkintlk
      301 lqqdivaqkq yeserrcafl lrrlrklqar ivgrhvaeen tgvlelahhg vkkylfqelv
      361 nissksnvrn fpeissslss flqkiekmcv aqsnsvnrqr lccryfgggs rdslgstsgn
      421 nsnrqpvfgt pqvkikgdev esvagplatq lhivesnlds dctasssgge scdemqsfnn
      481 lhqqhlsisk raawryaqdr agvaarwtwl qaqisdleyr irqhnelqrh vrankglvil
      541 dnaetvngys gvlpgstgry nspegelpas rtrpfvwsfy rkrkllqldr lhefskraak
      601 astvrcncdh vlppcalctg rsdpmqmqep ieqmsvqeri alvdpsfhpv lsfsddvtqg
      661 nhvdaimksv ewqqkmlrgn lritrlkdke tndrrnkklp ghrakyaarl kkssssilta
      721 rikrkmlkgk rnrlghdrsv hglsrkrvqk ltteddddis alsssskhss pvpsplhhta
      781 astekntike kssishgrlr qnsydidniv ipysvaastr leklqykeil tpkwrlceep
      841 lkldikngvm hrpsqdsdfe dmsdetialr hersereenk rfmtymtlph qsrirhnrrt
      901 dsradsgant pdpmsphasd fggdmmspit sppptpqvqd aehqqnldth rssalqnalr
      961 rrttptlrlg kddstlmned eneifpyepr vfplseevyd kmlevmpdgh wqassaslcs
     1021 reeekaddpf r
//
LOCUS       XP_034191093             983 aa            linear   INV 03-SEP-2025
DEFINITION  non-specific lethal 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191093
VERSION     XP_034191093.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335202.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..983
                     /product="non-specific lethal 1 isoform X3"
                     /calculated_mol_wt=109377
     Region          829..>916
                     /region_name="PEHE"
                     /note="PEHE domain; pfam15275"
                     /db_xref="CDD:464607"
     CDS             1..983
                     /gene="nsl1"
                     /coded_by="XM_034335202.2:1779..4730"
                     /db_xref="GeneID:117609176"
ORIGIN      
        1 mgvstahvkc rcgcvrtwep ldveaaalav svavmapalt eggpqkpenl lpplpasgfl
       61 speqatevcq nreilakfvv sanvqpskid dqclrgiskd lirdvinsky andldslspf
      121 thgsdlitkk glinqdceep dipgspekka tdiimndpsm gdqadnmgfl kssadlpmvg
      181 setagdnknl dvdqimafgt dittdneqcn mgnvgdigqn veeilqviks iegvenaesi
      241 sagggepvqs tvdgvemfsm peegfssfer dllndvdvms icnniniget vdqqkintlk
      301 lqqdivaqkq yeserrcafl lrrlrklqar ivgrhvaeen tgvlelahhg vkkylfqelv
      361 nissksnvrn fpeissslss flqkiekmcv aqsnsvnrqr lccryfgggs rdslgstsgn
      421 nsnrqpvfgt pqvkikgdev esvagplatq lhivesnlds dctasssgge scdemqsfnn
      481 lhqqhlsisk raawryaqdr agvaarwtwl qaqisdleyr irqhnelqrh vrankglvil
      541 dnaetvngys gvlpgstgry nspegelpas rtrpfvwsfy rkrkllqldr lhefskraak
      601 astvrcncdh vlppcalctg rsdpmqmqep ieqmsvqeri alvdpsfhpv lsfsddvtqg
      661 nhvdaimksv ewqqkmlrgn lritrlkdke tndrrnkklp ghrakyaarl kkssssilta
      721 rikrkmlkgk rnrlghdrsv hglsrkrvqk ltteddddis alsssskhss pvpsplhhta
      781 astekntike kssishgrlr qnsydidniv ipysvaastr leklqykeil tpkwrlceep
      841 lkldikngvm hrpsqdsdfe dmsdetialr hersereenk rfmtymtlph qsrirhnrrt
      901 dsradsgant pdpmsphasd fggdmmspit sppptpqvqd aehqqnldth rssalqnalr
      961 rrttptlrlg kddstlmned ene
//
LOCUS       XP_034191104             766 aa            linear   INV 03-SEP-2025
DEFINITION  protein on ecdysone puffs [Osmia lignaria lignaria].
ACCESSION   XP_034191104
VERSION     XP_034191104.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335213.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..766
                     /product="protein on ecdysone puffs"
                     /calculated_mol_wt=85640
     CDS             1..766
                     /gene="Pep"
                     /coded_by="XM_034335213.2:125..2425"
                     /db_xref="GeneID:117609195"
ORIGIN      
        1 msfnrgmkrd pfgnrsfsnr gggggggggs ggrvgnmggg gggmggnmgg gmgggggggg
       61 gggsgsggmn pweggmmpgr gilptpnnnl slaspqaqla iasnlltnll rtqqevqpqq
      121 vpsllslgnn fsgpgpnfpn qqnfssgrfn drpirhpmkn qrpqpynkmg nrardgpagr
      181 rgpsaqqsra pqsgnqrmng nqnqhrndks skpipaskqn qtakkeqqdv ktsdnekaea
      241 pvlkseeted pedmkrdwkd ekkevvdtdv dkneegdekp eedseqkgnk svtdesgkat
      301 gmtekdtkmt gkksearhae sryaevpmnh mfchicnkhm wdgysfenhl rgrahqlmme
      361 kldesyklkv dlmrhelrva eeqrelsltn skrrgkkvsv dlnvreyctm cdlnfygtls
      421 thrksekhqq lktflhprcf pclkefpsri eydehcltpa hmknavqcee qrknkkkekl
      481 akgeaevrta edeekdvgpd iknekeedae geqeyitdit enlsekkfki psykycrqnq
      541 vsigkslvkd vqgfycekcr rfmllpedmn ahlrsithyr nfvqevkalt ttaetteqkp
      601 sekselnesg lennedydgn wkrrkvtnse eedsmeikqe tdismddsna qkkadgdeky
      661 dpleadaese eeepreiesa seqssqnitd tpekktpvdk vwadidndnd aeignliddg
      721 eekehstdie ksvqkvdreq nppikpargr gfvrgrgspr arrarr
//
LOCUS       XP_034191136             444 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta subunit 2 [Osmia lignaria lignaria].
ACCESSION   XP_034191136
VERSION     XP_034191136.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335245.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191136.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..444
                     /product="DNA polymerase delta subunit 2"
                     /calculated_mol_wt=49604
     Region          45..174
                     /region_name="DNA_pol_D_N"
                     /note="DNA polymerase delta subunit OB-fold domain;
                     pfam18018"
                     /db_xref="CDD:436215"
     Region          193..443
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(199,201,242,287..288,334,397)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(199,201,242,287,334,397)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..444
                     /gene="LOC117609208"
                     /coded_by="XM_034335245.2:218..1552"
                     /db_xref="GeneID:117609208"
ORIGIN      
        1 mvhkdgksii eesenekpqv ferkqaqykd lskkfvntin dyskqfahiy airlaelrni
       61 liprvkakwg nipivrladl gniegqkcii igtlykhqqg kpsilrelse dhqltnpcsk
      121 sdycsdkdqa fledemlrir ligpevdlke ivtgvvcail gnenndgtft vkdwcppgca
      181 pkeplknyts egkliivsgl dlsansksle inlftewlcg magntvvqkd nasivrliia
      241 gntikrndtv nsatntlake igeaikttda flntlamcch itlmpgeydp tnamlpqkpl
      301 hpcllpkssr lesftsttnp wigkieermv mgtsgqsind iikatgetgi spiewlertl
      361 lwrhmcptap dtllacpyyk edlfimkecp dvyfvgntdk fetkllkgee neivrlisip
      421 cfstthtavi vnlqnldtky icfg
//
LOCUS       XP_076546505             155 aa            linear   INV 03-SEP-2025
DEFINITION  selenoprotein F [Osmia lignaria lignaria].
ACCESSION   XP_076546505
VERSION     XP_076546505.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690390.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="selenoprotein F"
                     /calculated_mol_wt=17792
     Region          78..152
                     /region_name="Sep15_SelM"
                     /note="Sep15/SelM redox domain; pfam08806"
                     /db_xref="CDD:462607"
     CDS             1..155
                     /gene="LOC117609213"
                     /coded_by="XM_076690390.1:220..687"
                     /db_xref="GeneID:117609213"
ORIGIN      
        1 mdflvllhif afsimmnivs tefsaddcks lgfnkanllc stceefnkre lteiwnncke
       61 cclkdddydt sglkryprai levctckfga ypqiqafiks nrpskyknlq ikyvrgldpi
      121 iklldadnkv edildihkwd tdsvdeflat hlsmd
//
LOCUS       XP_076546506             155 aa            linear   INV 03-SEP-2025
DEFINITION  selenoprotein F [Osmia lignaria lignaria].
ACCESSION   XP_076546506
VERSION     XP_076546506.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690391.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="selenoprotein F"
                     /calculated_mol_wt=17792
     Region          78..152
                     /region_name="Sep15_SelM"
                     /note="Sep15/SelM redox domain; pfam08806"
                     /db_xref="CDD:462607"
     CDS             1..155
                     /gene="LOC117609213"
                     /coded_by="XM_076690391.1:220..687"
                     /db_xref="GeneID:117609213"
ORIGIN      
        1 mdflvllhif afsimmnivs tefsaddcks lgfnkanllc stceefnkre lteiwnncke
       61 cclkdddydt sglkryprai levctckfga ypqiqafiks nrpskyknlq ikyvrgldpi
      121 iklldadnkv edildihkwd tdsvdeflat hlsmd
//
LOCUS       XP_076546507             155 aa            linear   INV 03-SEP-2025
DEFINITION  selenoprotein F [Osmia lignaria lignaria].
ACCESSION   XP_076546507
VERSION     XP_076546507.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690392.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="selenoprotein F"
                     /calculated_mol_wt=17792
     Region          78..152
                     /region_name="Sep15_SelM"
                     /note="Sep15/SelM redox domain; pfam08806"
                     /db_xref="CDD:462607"
     CDS             1..155
                     /gene="LOC117609213"
                     /coded_by="XM_076690392.1:220..687"
                     /db_xref="GeneID:117609213"
ORIGIN      
        1 mdflvllhif afsimmnivs tefsaddcks lgfnkanllc stceefnkre lteiwnncke
       61 cclkdddydt sglkryprai levctckfga ypqiqafiks nrpskyknlq ikyvrgldpi
      121 iklldadnkv edildihkwd tdsvdeflat hlsmd
//
LOCUS       XP_076546508             155 aa            linear   INV 03-SEP-2025
DEFINITION  selenoprotein F [Osmia lignaria lignaria].
ACCESSION   XP_076546508
VERSION     XP_076546508.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690393.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="selenoprotein F"
                     /calculated_mol_wt=17792
     Region          78..152
                     /region_name="Sep15_SelM"
                     /note="Sep15/SelM redox domain; pfam08806"
                     /db_xref="CDD:462607"
     CDS             1..155
                     /gene="LOC117609213"
                     /coded_by="XM_076690393.1:220..687"
                     /db_xref="GeneID:117609213"
ORIGIN      
        1 mdflvllhif afsimmnivs tefsaddcks lgfnkanllc stceefnkre lteiwnncke
       61 cclkdddydt sglkryprai levctckfga ypqiqafiks nrpskyknlq ikyvrgldpi
      121 iklldadnkv edildihkwd tdsvdeflat hlsmd
//
LOCUS       XP_076546509             155 aa            linear   INV 03-SEP-2025
DEFINITION  selenoprotein F [Osmia lignaria lignaria].
ACCESSION   XP_076546509
VERSION     XP_076546509.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690394.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="selenoprotein F"
                     /calculated_mol_wt=17792
     Region          78..152
                     /region_name="Sep15_SelM"
                     /note="Sep15/SelM redox domain; pfam08806"
                     /db_xref="CDD:462607"
     CDS             1..155
                     /gene="LOC117609213"
                     /coded_by="XM_076690394.1:220..687"
                     /db_xref="GeneID:117609213"
ORIGIN      
        1 mdflvllhif afsimmnivs tefsaddcks lgfnkanllc stceefnkre lteiwnncke
       61 cclkdddydt sglkryprai levctckfga ypqiqafiks nrpskyknlq ikyvrgldpi
      121 iklldadnkv edildihkwd tdsvdeflat hlsmd
//
LOCUS       XP_076546510            1122 aa            linear   INV 03-SEP-2025
DEFINITION  RAB11-binding protein RELCH homolog isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546510
VERSION     XP_076546510.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690395.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1122
                     /product="RAB11-binding protein RELCH homolog isoform X1"
                     /calculated_mol_wt=125102
     Region          454..482
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..522
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          531..558
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          569..595
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          857..885
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          895..925
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          934..963
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1122
                     /gene="LOC117609265"
                     /coded_by="XM_076690395.1:222..3590"
                     /db_xref="GeneID:117609265"
ORIGIN      
        1 madgvqevrd slptdskgcp vtkslisyee iaikllnekl lltalelhae lceagkelpi
       61 lrdffsnpnh fethnikpep ytpmprsssq atldsldmtr ysedgggvde rvailefelr
      121 karesisalr anltvvtese aatsdkgsdk qvvskppikp heqralnfli neyllarsyk
      181 ltsitfsden enqdfedwqd vglniskpae liqiyreymr angydkppsv sigvqtdfce
      241 iesetdkdef kemvnkveel kqqailleqe kldlqqiiat sgslsqnptk qdssgtiqtl
      301 nsssttpdkf elletpardt stvtqepeed dsvsvvvslg etdpgdkewt riqlprvdvt
      361 egssilpnpp srnlplkfkm eviahclvni pssiisttee lfkngvtrdt lvqilsqtlp
      421 rivpniilnr reeviplils airlhsdsae rekllqllfs lkkrpqeder qlilagfvam
      481 akledepmeg eeilticweq sqhkypekrl laveccsvlt pymsvgirns lmlsmlqqml
      541 ledkdptvra svvrslalli almddpdkyf qceelaltal hdispivvev assillpila
      601 qwalslkrlq thllpritsk iknhlklghs qhspnkdhid egrivssiav lqyllphtvi
      661 cvadteivkt yidhgissdl pdeflnlyhs nivnpkvfyd gdtnigalln tffantwend
      721 twpelewftn klvveiltmv ksidilqesi lnalltyihs lclgfgqyit etriqpifas
      781 evaeleqqlt tlstdkknms ltlipaylii lstlngtkvs nslkqflval smsgtsitsl
      841 qiaiiilcsq ehmqeyvlsg lwdgvvhqrp ivrcatatlf gcviahvsdr lasakvvpai
      901 vtlvsdsdvt vrsaaipslg rliteckvre vrdkarltle tiakepqgvp ptlalplvst
      961 lafiapncpq nyiedviaaq lvgitssalq qgrkidlina lveaysvlvy cplsnqcvsg
     1021 mlvpglkcle qlvnqylpqq keavrsllre aesrqdlskp merslsmssg lslsmatvnv
     1081 gqgvedmrqr vskifqqkts spsmtsifrk kywmyfdgpv yt
//
LOCUS       XP_076546511            1111 aa            linear   INV 03-SEP-2025
DEFINITION  RAB11-binding protein RELCH homolog isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546511
VERSION     XP_076546511.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690396.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1111
                     /product="RAB11-binding protein RELCH homolog isoform X2"
                     /calculated_mol_wt=123679
     Region          454..482
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          492..522
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          531..558
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          569..595
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          857..885
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          895..925
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          934..963
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1111
                     /gene="LOC117609265"
                     /coded_by="XM_076690396.1:222..3557"
                     /db_xref="GeneID:117609265"
ORIGIN      
        1 madgvqevrd slptdskgcp vtkslisyee iaikllnekl lltalelhae lceagkelpi
       61 lrdffsnpnh fethnikpep ytpmprsssq atldsldmtr ysedgggvde rvailefelr
      121 karesisalr anltvvtese aatsdkgsdk qvvskppikp heqralnfli neyllarsyk
      181 ltsitfsden enqdfedwqd vglniskpae liqiyreymr angydkppsv sigvqtdfce
      241 iesetdkdef kemvnkveel kqqailleqe kldlqqiiat sgslsqnptk qdssgtiqtl
      301 nsssttpdkf elletpardt stvtqepeed dsvsvvvslg etdpgdkewt riqlprvdvt
      361 egssilpnpp srnlplkfkm eviahclvni pssiisttee lfkngvtrdt lvqilsqtlp
      421 rivpniilnr reeviplils airlhsdsae rekllqllfs lkkrpqeder qlilagfvam
      481 akledepmeg eeilticweq sqhkypekrl laveccsvlt pymsvgirns lmlsmlqqml
      541 ledkdptvra svvrslalli almddpdkyf qceelaltal hdispivvev assillpila
      601 qwalslkrlq thllpritsk iknhlklghs qhspnkdhid egrivssiav lqyllphtvi
      661 cvadteivkt yidhgissdl pdeflnlyhs nivnpkvfyd gdtnigalln tffantwend
      721 twpelewftn klvveiltmv ksidilqesi lnalltyihs lclgfgqyit etriqpifas
      781 evaeleqqlt tlstdkknms ltlipaylii lstlngtkvs nslkqflval smsgtsitsl
      841 qiaiiilcsq ehmqeyvlsg lwdgvvhqrp ivrcatatlf gcviahvsdr lasakvvpai
      901 vtlvsdsdvt vrsaaipslg rliteckvre vrdkarltle tiakepqgvp ptlalplvst
      961 lafiapncpq nyiedviaaq lvgitssalq qgrkidlina lveaysvlvy cplsnqcvsg
     1021 mlvpglkcle qlvnqylpqq keavrsllre aesrqdlskp merslsmssg lslsmatvnv
     1081 gqgvedmrqr vskifqqkts spsmtsifrk k
//
LOCUS       XP_076546512            1039 aa            linear   INV 03-SEP-2025
DEFINITION  RAB11-binding protein RELCH homolog isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546512
VERSION     XP_076546512.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690397.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1039
                     /product="RAB11-binding protein RELCH homolog isoform X3"
                     /calculated_mol_wt=115800
     Region          371..399
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          409..439
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          448..475
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          486..512
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          774..802
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          812..842
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          851..880
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1039
                     /gene="LOC117609265"
                     /coded_by="XM_076690397.1:312..3431"
                     /db_xref="GeneID:117609265"
ORIGIN      
        1 mprsssqatl dsldmtryse dgggvderva ilefelrkar esisalranl tvvteseaat
       61 sdkgsdkqvv skppikpheq ralnfliney llarsyklts itfsdenenq dfedwqdvgl
      121 niskpaeliq iyreymrang ydkppsvsig vqtdfceies etdkdefkem vnkveelkqq
      181 ailleqekld lqqiiatsgs lsqnptkqds sgtiqtlnss sttpdkfell etpardtstv
      241 tqepeeddsv svvvslgetd pgdkewtriq lprvdvtegs silpnppsrn lplkfkmevi
      301 ahclvnipss iistteelfk ngvtrdtlvq ilsqtlpriv pniilnrree viplilsair
      361 lhsdsaerek llqllfslkk rpqederqli lagfvamakl edepmegeei lticweqsqh
      421 kypekrllav eccsvltpym svgirnslml smlqqmlled kdptvrasvv rslallialm
      481 ddpdkyfqce elaltalhdi spivvevass illpilaqwa lslkrlqthl lpritskikn
      541 hlklghsqhs pnkdhidegr ivssiavlqy llphtvicva dteivktyid hgissdlpde
      601 flnlyhsniv npkvfydgdt nigallntff antwendtwp elewftnklv veiltmvksi
      661 dilqesilna lltyihslcl gfgqyitetr iqpifaseva eleqqlttls tdkknmsltl
      721 ipayliilst lngtkvsnsl kqflvalsms gtsitslqia iiilcsqehm qeyvlsglwd
      781 gvvhqrpivr catatlfgcv iahvsdrlas akvvpaivtl vsdsdvtvrs aaipslgrli
      841 teckvrevrd karltletia kepqgvpptl alplvstlaf iapncpqnyi edviaaqlvg
      901 itssalqqgr kidlinalve aysvlvycpl snqcvsgmlv pglkcleqlv nqylpqqkea
      961 vrsllreaes rqdlskpmer slsmssglsl smatvnvgqg vedmrqrvsk ifqqktssps
     1021 mtsifrkkyw myfdgpvyt
//
LOCUS       XP_034191300             728 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase promoting complex subunit 5 ida [Osmia lignaria lignaria].
ACCESSION   XP_034191300
VERSION     XP_034191300.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335409.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="anaphase promoting complex subunit 5 ida"
                     /calculated_mol_wt=81946
     Region          22..159
                     /region_name="Apc5_N"
                     /note="N-terminal domain of the anaphase-promoting complex
                     subunit Apc5 (or Anapc5); cd16270"
                     /db_xref="CDD:293878"
     Site            order(34..35,45..46,49..50,52..53,57,115,124..127)
                     /site_type="other"
                     /note="Apc4 interaction interface [polypeptide binding]"
                     /db_xref="CDD:293878"
     Site            order(95..97,100,104,138,142..145,147..148)
                     /site_type="other"
                     /note="CDC23 interaction interface [polypeptide binding]"
                     /db_xref="CDD:293878"
     Site            order(129,132..133,136,139..140)
                     /site_type="other"
                     /note="APC subunit 15 interaction interface [polypeptide
                     binding]"
                     /db_xref="CDD:293878"
     Region          228..262
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276806"
     Region          234..327
                     /region_name="ANAPC5"
                     /note="Anaphase-promoting complex subunit 5; pfam12862"
                     /db_xref="CDD:432838"
     Region          277..310
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276806"
     CDS             1..728
                     /gene="ida"
                     /coded_by="XM_034335409.2:277..2463"
                     /db_xref="GeneID:117609274"
ORIGIN      
        1 msvdlmnidg nikrvqketl tpykvatvil ikeycnettk aiverrdfcl aalkliqspd
       61 mefnallnil yspeyilhrf ahqlevqlnv lkgkgvegll dlfdsvgrlm kptidlalpa
      121 lnknsvlgly irrviiffek lpfdqvvaly ealkkylerr snasessgic mgskaedsys
      181 neikttwggr qaellvaqqa halqtdehka mppaelqvlv rellscspyy aeahylsyln
      241 clrvneycga vdslyhcfdr laamenrsap edrsrifrya alnlavlhaq fnhkevaqaa
      301 lkesimmaqe agdnvclqla hawmyylttr qkgpliersv gkasmlgith ttglgliaya
      361 hnsaleaknp sqvfetltks dvlncqhsmt dlmsityaek calwayygkt emssvcgqll
      421 llhntgdkkq hmfngpstcq avvnianilv efgeysmvdi vlahakdrfp nepcnkiwml
      481 seqlyvftql trqekwseae aialnissld lleckfrlae vylgkgdypk glecingiek
      541 deqitpqnki rtmilssqim casvspenri agsnslvlln talelavknh lsyyealikm
      601 hlaniqlimn mptlavnlve eviiqilahg gyydqgrafv lyakclvata pandkarkev
      661 ilkaiktlsk akdyfmkvea ygrlrttlcl esllcheidl kternqcafe frqldqqllt
      721 kldnlsly
//
LOCUS       XP_034191313             499 aa            linear   INV 03-SEP-2025
DEFINITION  lysophosphatidylcholine acyltransferase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191313
VERSION     XP_034191313.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335422.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191313.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..499
                     /product="lysophosphatidylcholine acyltransferase isoform
                     X1"
                     /calculated_mol_wt=56183
     Region          101..314
                     /region_name="LPLAT_LPCAT1-like"
                     /note="Lysophospholipid Acyltransferases (LPLATs) of
                     Glycerophospholipid Biosynthesis: LPCAT1-like; cd07991"
                     /db_xref="CDD:153253"
     Site            order(133,136,138,152..155,208..210)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153253"
     Region          <389..474
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..499
                     /gene="LPCAT"
                     /coded_by="XM_034335422.2:355..1854"
                     /db_xref="GeneID:117609281"
ORIGIN      
        1 maeangkmln khedidaasl gadilnpfvh rleldttydk lktafltval lpfrlaaita
       61 lvimawllac lgllglseed lrrapltgwr rdmrivicwv mralficggf hhlkvkgrka
      121 etkdapvlal aphssffdal pvvylggpsi vakaeigrip ffgklinytq pvyvwredpn
      181 srqntikeii eratskedwp qvmifpegtc tnrsclitfk sgafypgvpv qpvcirypnk
      241 ldtvtwtweg pgalkllwlt ltqlnsscei eflpvykpse aektdpklya nnvrrlmaea
      301 lqipvsdyty ddcriitkah qlhiphasti veahklrnkl glvtakteee lvqkkterfs
      361 eemnlhefaq ilridekepa tqqlfrihdk qakgiidfee ylfivlatak anselekvet
      421 afevcgtksl sclnkmelrk alklalslpe eesdkifqna kidsadttvn fefvlaalaa
      481 raelahifvg ntetrkkni
//
LOCUS       XP_034191322             498 aa            linear   INV 03-SEP-2025
DEFINITION  lysophosphatidylcholine acyltransferase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191322
VERSION     XP_034191322.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335431.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191322.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="lysophosphatidylcholine acyltransferase isoform
                     X2"
                     /calculated_mol_wt=56136
     Region          108..313
                     /region_name="LPLAT_LPCAT1-like"
                     /note="Lysophospholipid Acyltransferases (LPLATs) of
                     Glycerophospholipid Biosynthesis: LPCAT1-like; cd07991"
                     /db_xref="CDD:153253"
     Site            order(132,135,137,151..154,207..209)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153253"
     Region          <388..473
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..498
                     /gene="LPCAT"
                     /coded_by="XM_034335431.2:356..1852"
                     /db_xref="GeneID:117609281"
ORIGIN      
        1 maeangkmln khedidaasl gadilnpfvh rleldttydk lktafltval lpfrlaaita
       61 lvimawllac lgllglseed lrrapltgwr rkivpwlcff grltyqaggm riivrgrqat
      121 raeapilvva phstfmdggi vyitgfpsii vrresglnpf igklinytqp vyvwredpns
      181 rqntikeiie ratskedwpq vmifpegtct nrsclitfks gafypgvpvq pvcirypnkl
      241 dtvtwtwegp galkllwltl tqlnssceie flpvykpsea ektdpklyan nvrrlmaeal
      301 qipvsdytyd dcriitkahq lhiphastiv eahklrnklg lvtakteeel vqkkterfse
      361 emnlhefaqi lridekepat qqlfrihdkq akgiidfeey lfivlataka nselekveta
      421 fevcgtksls clnkmelrka lklalslpee esdkifqnak idsadttvnf efvlaalaar
      481 aelahifvgn tetrkkni
//
LOCUS       XP_034191348             426 aa            linear   INV 03-SEP-2025
DEFINITION  dipeptidase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034191348
VERSION     XP_034191348.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335457.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="dipeptidase 1"
                     /calculated_mol_wt=47028
     Region          50..383
                     /region_name="Peptidase_M19"
                     /note="Membrane dipeptidase (Peptidase family M19);
                     pfam01244"
                     /db_xref="CDD:395996"
     Site            order(60,62,116,173,244,265,276,334,337)
                     /site_type="active"
                     /db_xref="CDD:238626"
     Site            order(118,127..129,131..132,135,139,183..184,186..187,
                     190..191)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238626"
     CDS             1..426
                     /gene="LOC117609298"
                     /coded_by="XM_034335457.2:314..1594"
                     /db_xref="GeneID:117609298"
ORIGIN      
        1 mrcvgqsgvw tasviilsig swigkvgprp adgeishlts kerletvrqv lsevplidgh
       61 ndlpwnirnf vhnqladfdf dkdlrqvapw sksawsqtdl vrlrqgmvgg qfwaayvpce
      121 sqhlnavqlt leqvdlikrl iekysqdmqf aassreilea hgkgriasli gvegghslgs
      181 slavlrtlyq lgvryltlth tcntpwakss svedededgg gltafgravv qemnrlgmli
      241 dlshtaratm rdalrasrap lifshssafa icnssrnvpd dilrqladng glvmvtfyny
      301 fvkcgsqasv sdvaehiyyi rnligvdhig vggdfdgink tprgledvsk ypelfaellr
      361 sgkwnvldlk kvaglnllrv mrqvervrdd mrtagvtpse eylqdspdti gscaldinsv
      421 qrvmev
//
LOCUS       XP_034191355             426 aa            linear   INV 03-SEP-2025
DEFINITION  dipeptidase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034191355
VERSION     XP_034191355.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335464.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="dipeptidase 1"
                     /calculated_mol_wt=47028
     Region          50..383
                     /region_name="Peptidase_M19"
                     /note="Membrane dipeptidase (Peptidase family M19);
                     pfam01244"
                     /db_xref="CDD:395996"
     Site            order(60,62,116,173,244,265,276,334,337)
                     /site_type="active"
                     /db_xref="CDD:238626"
     Site            order(118,127..129,131..132,135,139,183..184,186..187,
                     190..191)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238626"
     CDS             1..426
                     /gene="LOC117609298"
                     /coded_by="XM_034335464.2:314..1594"
                     /db_xref="GeneID:117609298"
ORIGIN      
        1 mrcvgqsgvw tasviilsig swigkvgprp adgeishlts kerletvrqv lsevplidgh
       61 ndlpwnirnf vhnqladfdf dkdlrqvapw sksawsqtdl vrlrqgmvgg qfwaayvpce
      121 sqhlnavqlt leqvdlikrl iekysqdmqf aassreilea hgkgriasli gvegghslgs
      181 slavlrtlyq lgvryltlth tcntpwakss svedededgg gltafgravv qemnrlgmli
      241 dlshtaratm rdalrasrap lifshssafa icnssrnvpd dilrqladng glvmvtfyny
      301 fvkcgsqasv sdvaehiyyi rnligvdhig vggdfdgink tprgledvsk ypelfaellr
      361 sgkwnvldlk kvaglnllrv mrqvervrdd mrtagvtpse eylqdspdti gscaldinsv
      421 qrvmev
//
LOCUS       XP_034191383             425 aa            linear   INV 03-SEP-2025
DEFINITION  cGAS-like receptor 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191383
VERSION     XP_034191383.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335492.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191383.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="cGAS-like receptor 1 isoform X1"
                     /calculated_mol_wt=49456
     Region          69..266
                     /region_name="Mab-21"
                     /note="Mab-21 protein nucleotidyltransferase domain;
                     pfam03281"
                     /db_xref="CDD:460873"
     Region          272..349
                     /region_name="Mab-21_C"
                     /note="Mab-21 protein HhH/H2TH-like domain; pfam20266"
                     /db_xref="CDD:466416"
     CDS             1..425
                     /gene="cGlr1"
                     /coded_by="XM_034335492.2:356..1633"
                     /db_xref="GeneID:117609317"
ORIGIN      
        1 mdnaqlkkcf sddtvfnlin kmfitlqdse vkrnnvylel vvktliermk smnslfekay
       61 keiifcgsfy kgtkvghpne fdlniilklp iicdsinfsq vepafiqilv kensfasnyn
      121 nlsnkeqrll rtfmyngfln pdkfrqwveg iiskviselp rvgdkhclev knvsanfttr
      181 ikvkksgpaf tlmlnipqen deihidlvpa lafnihllng yfkkltelhg yqnktcyaip
      241 lplkdinsda thwrlsfcnq ekellskygr ikpiirqmkk lrdmqnwksi asyfietlfl
      301 ntlnksemdl dkipstllfy mmlkelhqac eqhkipffwd drynllgkiq hiemfnissr
      361 mnniikmiek nimtnkfila eyiltkheme lliakqslih mslpaysssn kgdepqengs
      421 icsil
//
LOCUS       XP_034191392             368 aa            linear   INV 03-SEP-2025
DEFINITION  cGAS-like receptor 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191392
VERSION     XP_034191392.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335501.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191392.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..368
                     /product="cGAS-like receptor 1 isoform X2"
                     /calculated_mol_wt=42923
     Region          37..209
                     /region_name="Mab-21"
                     /note="Mab-21 protein nucleotidyltransferase domain;
                     pfam03281"
                     /db_xref="CDD:460873"
     Region          215..292
                     /region_name="Mab-21_C"
                     /note="Mab-21 protein HhH/H2TH-like domain; pfam20266"
                     /db_xref="CDD:466416"
     CDS             1..368
                     /gene="cGlr1"
                     /coded_by="XM_034335501.2:357..1463"
                     /db_xref="GeneID:117609317"
ORIGIN      
        1 mdnaqlkkcf sddtvfnlin kmfitlqdse vkrnnvylel fsqvepafiq ilvkensfas
       61 nynnlsnkeq rllrtfmyng flnpdkfrqw vegiiskvis elprvgdkhc levknvsanf
      121 ttrikvkksg paftlmlnip qendeihidl vpalafnihl lngyfkklte lhgyqnktcy
      181 aiplplkdin sdathwrlsf cnqekellsk ygrikpiirq mkklrdmqnw ksiasyfiet
      241 lflntlnkse mdldkipstl lfymmlkelh qaceqhkipf fwddrynllg kiqhiemfni
      301 ssrmnniikm ieknimtnkf ilaeyiltkh emelliakqs lihmslpays ssnkgdepqe
      361 ngsicsil
//
LOCUS       XP_034191406             340 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609325 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191406
VERSION     XP_034191406.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335515.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="uncharacterized protein LOC117609325 isoform X1"
                     /calculated_mol_wt=38636
     Region          19..187
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(23..26,120..121,124..126,182)
                     /site_type="active"
                     /db_xref="CDD:99839"
     Site            order(23,25,126,182)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99839"
     Site            order(24..26,120..121,124..125,182)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:99839"
     CDS             1..340
                     /gene="LOC117609325"
                     /coded_by="XM_034335515.2:377..1399"
                     /db_xref="GeneID:117609325"
ORIGIN      
        1 mhgiklhald qslkmiktfi ffdlettgli erkvmpkiie lsliavsrna icetmdplpr
       61 vlhklvlpvh pntdisklih nltglsnknl eevqyfnyea cnlvmnfinr qtpptcfvay
      121 ngnnfdypif lselkninkd vsgdvlsidm lnlikdffsl kenaienrnd iqttssmsnk
      181 ediksllndg ydkalcdald svmnssfkky nknnhenkes nssaanilnt pktscykkiq
      241 kmnektpenq viksqdmdkc lhkrkesiar rklhftsgqp vnfklssvck hifgtdpqna
      301 htaegdclsm ircaiqlgny fvewadckat plinydkeke
//
LOCUS       XP_034191414             326 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609325 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191414
VERSION     XP_034191414.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335523.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="uncharacterized protein LOC117609325 isoform X2"
                     /calculated_mol_wt=37063
     Region          5..173
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     Site            order(9..12,106..107,110..112,168)
                     /site_type="active"
                     /db_xref="CDD:99839"
     Site            order(9,11,112,168)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99839"
     Site            order(10..12,106..107,110..111,168)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:99839"
     CDS             1..326
                     /gene="LOC117609325"
                     /coded_by="XM_034335523.2:339..1319"
                     /db_xref="GeneID:117609325"
ORIGIN      
        1 miktfiffdl ettglierkv mpkiielsli avsrnaicet mdplprvlhk lvlpvhpntd
       61 isklihnltg lsnknleevq yfnyeacnlv mnfinrqtpp tcfvayngnn fdypiflsel
      121 kninkdvsgd vlsidmlnli kdffslkena ienrndiqtt ssmsnkedik sllndgydka
      181 lcdaldsvmn ssfkkynknn henkesnssa anilntpkts cykkiqkmne ktpenqviks
      241 qdmdkclhkr kesiarrklh ftsgqpvnfk lssvckhifg tdpqnahtae gdclsmirca
      301 iqlgnyfvew adckatplin ydkeke
//
LOCUS       XP_034191421             297 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609325 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191421
VERSION     XP_034191421.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335530.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..297
                     /product="uncharacterized protein LOC117609325 isoform X3"
                     /calculated_mol_wt=33738
     Region          3..144
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     CDS             1..297
                     /gene="LOC117609325"
                     /coded_by="XM_034335530.2:379..1272"
                     /db_xref="GeneID:117609325"
ORIGIN      
        1 mhvsrnaice tmdplprvlh klvlpvhpnt disklihnlt glsnknleev qyfnyeacnl
       61 vmnfinrqtp ptcfvayngn nfdypiflse lkninkdvsg dvlsidmlnl ikdffslken
      121 aienrndiqt tssmsnkedi ksllndgydk alcdaldsvm nssfkkynkn nhenkesnss
      181 aanilntpkt scykkiqkmn ektpenqvik sqdmdkclhk rkesiarrkl hftsgqpvnf
      241 klssvckhif gtdpqnahta egdclsmirc aiqlgnyfve wadckatpli nydkeke
//
LOCUS       XP_034191430             286 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609325 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191430
VERSION     XP_034191430.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335539.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="uncharacterized protein LOC117609325 isoform X4"
                     /calculated_mol_wt=32495
     Region          <4..133
                     /region_name="DnaQ_like_exo"
                     /note="DnaQ-like (or DEDD) 3'-5' exonuclease domain
                     superfamily; cl10012"
                     /db_xref="CDD:447876"
     CDS             1..286
                     /gene="LOC117609325"
                     /coded_by="XM_034335539.2:328..1188"
                     /db_xref="GeneID:117609325"
ORIGIN      
        1 mdplprvlhk lvlpvhpntd isklihnltg lsnknleevq yfnyeacnlv mnfinrqtpp
       61 tcfvayngnn fdypiflsel kninkdvsgd vlsidmlnli kdffslkena ienrndiqtt
      121 ssmsnkedik sllndgydka lcdaldsvmn ssfkkynknn henkesnssa anilntpkts
      181 cykkiqkmne ktpenqviks qdmdkclhkr kesiarrklh ftsgqpvnfk lssvckhifg
      241 tdpqnahtae gdclsmirca iqlgnyfvew adckatplin ydkeke
//
LOCUS       XP_034191465            1055 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol glycan anchor biosynthesis class O [Osmia
            lignaria lignaria].
ACCESSION   XP_034191465
VERSION     XP_034191465.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1055
                     /product="phosphatidylinositol glycan anchor biosynthesis
                     class O"
                     /calculated_mol_wt=120235
     Region          72..339
                     /region_name="GPI_EPT_3"
                     /note="GPI ethanolamine phosphate transferase 3, PIG-O;
                     cd16023"
                     /db_xref="CDD:293747"
     Site            order(79,130,232,274..275)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293747"
     Site            order(79,130,232,275)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293747"
     CDS             1..1055
                     /gene="PIG-O"
                     /coded_by="XM_034335574.2:282..3449"
                     /db_xref="GeneID:117609341"
ORIGIN      
        1 msklwnylif qgwisylmaa gllvftsgfl lnrisrpera eckyctdsdc nieqllqdpq
       61 vagticverk trvvllvvda lkydfaywyn dnsstssyyr nklpiihell enqpmnsrly
      121 kfiadppttt mqrlkglttg slptfidigs nfasesided nivdqntarg iifmgddtwt
      181 nlfpgkfkrq fpspsfnvwd ldtvdkdvry riffemkkkd wslliahvlg idhcghkhgt
      241 nhpemarkln dtntlikeii esleedtvlf vvgdhgmtet gdhggdsine ieaamfvysm
      301 iplvqykaah stvnqvdlvp tlasvlgtpi pfsnlgsvii nslpssaknk rseddlwysl
      361 hstwrnivqt kkyidvystd sylfsndqlq slenmynhlf eqikhvntve efesfvmnsr
      421 nyfkllkntc sevwiqfdss lmskglllmf calfffylfi tgipesrmsk ifqssflqcs
      481 ilinlitalg iiflflfnvl eelkttllsa tgavsvvllv fliaknwdvi smswydyrki
      541 kkliyisrvi llltvcilfs nsyiveednv lsfllvtlfw llvfnlkken tnegierktk
      601 pffksqtksn lktvvivigl iasisirlsy yfwrcreeqq trvcslfvig kmgsiisnnl
      661 ervllaiali vlalyitiir lwlqncgnls gfspsvlvgq ycpvvigvcm gcywvlqrlp
      721 kfikikfals wqistlpniv yalclfaifi lyyrplsifl lpkrkesinv yqdenivprl
      781 fekikesiyr knidvdetpv iyglgtaysa tfvslsvflm llyslllgdi lspstflmfi
      841 scasvlglsa ieryknansi selvevptpt llcwfliaey ffygtghqst fptihwdaaf
      901 igtgghfygn llsailigin tfgshiilga tlpllvivpf tfylvfpkla kvkfveddmk
      961 rgelllfeqd sifhaaifsv agkyillygi rtfgsmlaat ihcrhlmvwk ifapklifeg
     1021 lgllvtlssv lasfymvfri dqqmehlisr vakgr
//
LOCUS       XP_034191477             641 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609349 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191477
VERSION     XP_034191477.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335586.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="uncharacterized protein LOC117609349 isoform X1"
                     /calculated_mol_wt=70075
     CDS             1..641
                     /gene="LOC117609349"
                     /coded_by="XM_034335586.2:49..1974"
                     /db_xref="GeneID:117609349"
ORIGIN      
        1 mfllkdlllf syltvliaga avkiptktpw tssasksvys raigppmgmd titvpymsks
       61 pffpkfvdpk mmiskktdll snlfgglgpa ypagfkpymp ygasgpslys aadatqmfna
      121 msknsadegn gpykrgifgp ysakpfgpms skfgpmskys glntipdysm enefsrkrrs
      181 idespvaklr simdagmpet knlgppppyp tlapiseepe devnvllkkm gggtapkqyl
      241 pgmfgpfgpv vdpsmfmakk safldtlfkn latsttpspa dtvtatstek stivppdfwf
      301 ptsiiptpte yndkveqfmd klfetlkink taassedesa fkndftrsat fdedvvqski
      361 arsaedlssi naakdtivdt ilaelgdlks nmvttmndli syekaasaas pmsskkpfks
      421 fwpgmkpvpn gmvpfqqkma ilsqvfdmlt dlqknitlai qnivkakmss vapqgsgtyp
      481 smlndfttpv nmslldaikq rldaldygmp maynpsfsqf dskmarslpk gpssfwvsyp
      541 edganikrqv dsdteslisk nansdqkqqa rsvkmqmhqg yqslppgaie svqagggsvp
      601 ghqgggiklf ddnnyddtnq wvnwmeylkn nyhghrhhnh h
//
LOCUS       XP_034191485             631 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609349 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191485
VERSION     XP_034191485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..631
                     /product="uncharacterized protein LOC117609349 isoform X2"
                     /calculated_mol_wt=68474
     CDS             1..631
                     /gene="LOC117609349"
                     /coded_by="XM_034335594.2:49..1944"
                     /db_xref="GeneID:117609349"
ORIGIN      
        1 mfllkdlllf syltvliaga avkiptktpw tssasksvys raigppmgmd titvpymsks
       61 pffpkfvdpk mmiskktdll snlfgglgpa ypagfkpymp ygasgpslys aadatqmfna
      121 msknsadegn gpykrgifgp ysakpfgpms skfgpmskys glntipdysm enefsrkrrs
      181 idespvaklr simdagmpet knlgppppyp tlapiseepe devnvllkkm gggtapkqyl
      241 pgmfgpfgpv vdpsmfmakk safldtlfkn latsttpspa dtvtatstek stivppdfwf
      301 ptsiiptpte yndkveqfmd klfetlkink taassedesa fkndftrsat fdedvvqski
      361 arsaedlssi naakdtivdt ilaelgdlks nmvttmndli syekaasaas pmsskkpfks
      421 fwpgmkpvpn gmvpfqqkma ilsqvfdmlt dlqknitlai qnivkakmss vapqgsgtyp
      481 smlndfttpv nmslldaikq rldaldygmp maynpsfsqf dskmarslpk gpssfwvsyp
      541 edganikrqv dsdteslisk nansdqkqqa rsvkmqmhqg yqslppgaie svqagggsvp
      601 ghqgggiklf iqmpalpnml vpgfqnnnyk n
//
LOCUS       XP_034191499             630 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609360 [Osmia lignaria lignaria].
ACCESSION   XP_034191499
VERSION     XP_034191499.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335608.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="uncharacterized protein LOC117609360"
                     /calculated_mol_wt=73230
     CDS             1..630
                     /gene="LOC117609360"
                     /coded_by="XM_034335608.2:335..2227"
                     /db_xref="GeneID:117609360"
ORIGIN      
        1 meiknstdmi eengnttvnv astfenypfs wialqqnsky knvinainql lsmyhpddgi
       61 nayehvlktl lvlsenfdsn mqeyiisiip lpkgasdiai kdiicktidl iksannmaay
      121 qnsvpenkfn tegldekskm hlnqnilvnt ssqnfefiss vpevfrgfpp neismihshv
      181 knvnyvyntl dnniphieac inikqnflsn vneeeeslkl qsklewlqtp hfqlsidkfn
      241 nynhcnianv prtlyqddty fkrkfykngv ilnkqdkngi snlrgthnsk yslksemnck
      301 avynedinqd lkknkiinee mykhyvqelr neqviqqdiy kmdvvcydek ekdtvmnskt
      361 leennfitnn fvahrklkks lysflkraeq eilievpifp qiksvhiapp tskcwdsvte
      421 knlenkneld fllntkkyvn vseninkyte kkplrsmniy pksaqlqvlh rnlntvigkt
      481 qaiecekkdn iehlqykytv kqtkqnncnt lqqsasitkl atfkkqyydt llniqkakva
      541 vansmalssv etstkydpcy sahlyqqqll qkheqltvnp qfavhsvtqs glsnmyntpy
      601 twvqydwrhp kakqlrfsqe fplqlsrnre
//
LOCUS       XP_034191509             518 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609367 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191509
VERSION     XP_034191509.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335618.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..518
                     /product="uncharacterized protein LOC117609367 isoform X1"
                     /calculated_mol_wt=57905
     Region          118..253
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..518
                     /gene="LOC117609367"
                     /coded_by="XM_034335618.2:393..1949"
                     /db_xref="GeneID:117609367"
ORIGIN      
        1 msetrhhlhs vsdlyaadei evllleeird lelpasaysr pediqdfdia gsrtggqiss
       61 qpleldspgv hstvhswdsh anyhghygyg dhhdtssnal awprtshlvl ycdiqghlkt
      121 aigvvrllll issaaclatl cssgtakvsl fmlplvgrlr fmifvavfcf litalllfld
      181 ishviyvfpf nwaklnawvf tgiglsyals sallacsiwe yysggwvpkr trsqlsaaav
      241 lglscailaf llswihgrsr sscrnpdsrn htptqlykpv dnssssgvtl kerspkrpds
      301 wvvknqqskk qntdsdtntn nghhgndths rykqaakrkd hwasarqhfl pseennediq
      361 rddvnterrr rrrrrngdss stgdgnlisn ktdsdhiied nkprrvprkl alqsveqsrp
      421 wpgaergsss smqirnktlq ipvnnnaqdy cgidesssmq vtqngfhwem ectssisiek
      481 ttteiamdrt amwsgqwhpp pddvqpcssk tidpysfa
//
LOCUS       XP_034191519             517 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609367 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191519
VERSION     XP_034191519.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335628.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..517
                     /product="uncharacterized protein LOC117609367 isoform X2"
                     /calculated_mol_wt=57834
     Region          117..252
                     /region_name="MARVEL"
                     /note="Membrane-associating domain; pfam01284"
                     /db_xref="CDD:366555"
     CDS             1..517
                     /gene="LOC117609367"
                     /coded_by="XM_034335628.2:394..1947"
                     /db_xref="GeneID:117609367"
ORIGIN      
        1 msetrhhlhs vsdlyaadei evllleeird lelpasaysr pediqdfdig srtggqissq
       61 pleldspgvh stvhswdsha nyhghygygd hhdtssnala wprtshlvly cdiqghlkta
      121 igvvrlllli ssaaclatlc ssgtakvslf mlplvgrlrf mifvavfcfl italllfldi
      181 shviyvfpfn waklnawvft giglsyalss allacsiwey ysggwvpkrt rsqlsaaavl
      241 glscailafl lswihgrsrs scrnpdsrnh tptqlykpvd nssssgvtlk erspkrpdsw
      301 vvknqqskkq ntdsdtntnn ghhgndthsr ykqaakrkdh wasarqhflp seennediqr
      361 ddvnterrrr rrrrngdsss tgdgnlisnk tdsdhiiedn kprrvprkla lqsveqsrpw
      421 pgaergssss mqirnktlqi pvnnnaqdyc gidesssmqv tqngfhweme ctssisiekt
      481 tteiamdrta mwsgqwhppp ddvqpcsskt idpysfa
//
LOCUS       XP_034187688            1799 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 9
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034187688
VERSION     XP_034187688.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331797.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1799
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 9 isoform X1"
                     /calculated_mol_wt=204323
     Region          132..253
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          380..590
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(527..528,531,537)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          606..673
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          685..737
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          741..844
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          846..963
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          972..1024
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1029..1087
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1092..1144
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1147..1198
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1201..1252
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1296..1343
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1346..1399
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1418..1478
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1482..1539
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1545..1599
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1601..1796
                     /region_name="GON"
                     /note="GON domain; pfam08685"
                     /db_xref="CDD:462559"
     CDS             1..1799
                     /gene="LOC117607743"
                     /coded_by="XM_034331797.2:746..6145"
                     /db_xref="GeneID:117607743"
ORIGIN      
        1 mcsagrgvvd msrisnrast iaailsilll vvflvvlwtg irtsnsrasg ietfdgfwke
       61 ilnetttgra vfdeesfsne vipdikktsr eqndisndas smhpdleyvs lkkisenqye
      121 dppdefstak rhhsghfrhr taevwdphpq yeftafgrrf rlrlahdtsf vspnirithm
      181 tengtrkehp ghelgcfysg tvdddpssvv svslchgmtg hvrtstgsyi ikpveswqen
      241 dkdprefslq haiqrirspn adhpnndekk srthscavid ddtptpildd mdgsqiyvgg
      301 rtrrrrslte ktaldhsqin esyeqfignn eehrnfirnd ryysverrrn easeeysqds
      361 dmesdpfvtw rprralprey fieimvaada emvryhgkgl fsyilvlmsm vsriykdrsi
      421 gnpinisvmk iiptdkmfga rhtvsegiaa admlerfchw qknnnpdeps rehhdlalll
      481 trenlchpee sscdtlglak lgrmcspiss caivqdngla avftiaheig hvfnmphdde
      541 akcegfrnhs gfhhvmsrml naqtfpwews ncsrhyvtef leagygncll depditmege
      601 dvsrlpgedf sgnkqcelvf gpgswicsdm vsdvcktlwc hvphsvrcrt syspwadgtv
      661 cgenrwchrg kcvsrinlqp vdgqwgewgr ygecsrtcgg gikkkyrecn npppqnygky
      721 cvgervkyrs catkecpgnh dfreqqcsqf dnnnlnilnl tkdvkwhaky rgilpperck
      781 lycqvasnqy ymlrekvidg tpcgrdtfhv cvnghckaag cdhvlnstae ldicgvckgd
      841 nstcqritgs ynstefgytr vakipagskd idirqhgwlg mgddnnylal rlgeggeyil
      901 ngnfmvmpqk iiirpgltie ysgpvspver lntsepidtd lilevlsvfn lyppqityey
      961 tvpkhilhsy twilsdwsec thtcqgmkyr kaecrstenk dvvpddychn teklreesqi
     1021 cnnhctlkwe itsvsecssq cgpgtrtvts rclqklinsd hpprpiptra cahlprpsek
     1081 epcigpcanv hwnyskwstc svtcgggvqy rtavcvdsnw rrvsdescar qekhlkricg
     1141 qdpcpqwdlg kwhscsvtcg igkrhrdylc hvenrivphs ycgdpppgvv evcnagpceq
     1201 wqagiwspcs vtcgegikrr kvickhsdrs isdkcppsek pedtticilk pcptvvnepp
     1261 ikyssdpphg ihsqqnnevy ditfrsgykw hgsqkecsrp ciggymhtmv kcisieteii
     1321 apdhycdrke kpptmipcnr hrcpiwntgd wsqcdvecgt gfqhrqvrcq sprgeilade
     1381 rchdsekpei vktcrkspci tgptdrknrl etnifrkwkv sswtpcsksc gsglqrrrve
     1441 ctmrrgnhgp evtvkdeqcs rlglskprsq rpcrrvacdy iwqegpwsec saqcgegiqs
     1501 ravtchranh hgmidptptd ncpmdqkpss qqtcklrecd dkyywttgqw tkcshtcgqk
     1561 grqtrklfch detgkkvarv ncprefkpqr krkcnqrkcy pmscleakky ykttkdseyp
     1621 liiggrsmsi ychemstsep keyltlrssf wenyaevydk sllhphtcpy ngerndscnc
     1681 atvngtisgk tiyrriridp vklyvidndy tfswtngtnp vkygtagdcy sqaecpqgrf
     1741 sinlsgtqfk lssdvvwprr mgsvveinki nsqrvlgkcg gycgycspkp glkldvlpp
//
LOCUS       XP_034187699            1797 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 9
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034187699
VERSION     XP_034187699.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331808.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1797
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 9 isoform X2"
                     /calculated_mol_wt=204151
     Region          132..253
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          380..590
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(527..528,531,537)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          606..671
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          683..735
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          739..842
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          844..961
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          970..1022
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1027..1085
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1090..1142
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1145..1196
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1199..1250
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1294..1341
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1344..1397
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1416..1476
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1480..1537
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1543..1597
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1599..1794
                     /region_name="GON"
                     /note="GON domain; pfam08685"
                     /db_xref="CDD:462559"
     CDS             1..1797
                     /gene="LOC117607743"
                     /coded_by="XM_034331808.2:746..6139"
                     /db_xref="GeneID:117607743"
ORIGIN      
        1 mcsagrgvvd msrisnrast iaailsilll vvflvvlwtg irtsnsrasg ietfdgfwke
       61 ilnetttgra vfdeesfsne vipdikktsr eqndisndas smhpdleyvs lkkisenqye
      121 dppdefstak rhhsghfrhr taevwdphpq yeftafgrrf rlrlahdtsf vspnirithm
      181 tengtrkehp ghelgcfysg tvdddpssvv svslchgmtg hvrtstgsyi ikpveswqen
      241 dkdprefslq haiqrirspn adhpnndekk srthscavid ddtptpildd mdgsqiyvgg
      301 rtrrrrslte ktaldhsqin esyeqfignn eehrnfirnd ryysverrrn easeeysqds
      361 dmesdpfvtw rprralprey fieimvaada emvryhgkgl fsyilvlmsm vsriykdrsi
      421 gnpinisvmk iiptdkmfga rhtvsegiaa admlerfchw qknnnpdeps rehhdlalll
      481 trenlchpee sscdtlglak lgrmcspiss caivqdngla avftiaheig hvfnmphdde
      541 akcegfrnhs gfhhvmsrml naqtfpwews ncsrhyvtef leagygncll depditmege
      601 dvsrlpgedf sgnkqcelvf gpgswicsdm evcktlwchv phsvrcrtsy spwadgtvcg
      661 enrwchrgkc vsrinlqpvd gqwgewgryg ecsrtcgggi kkkyrecnnp ppqnygkycv
      721 gervkyrsca tkecpgnhdf reqqcsqfdn nnlnilnltk dvkwhakyrg ilpperckly
      781 cqvasnqyym lrekvidgtp cgrdtfhvcv nghckaagcd hvlnstaeld icgvckgdns
      841 tcqritgsyn stefgytrva kipagskdid irqhgwlgmg ddnnylalrl geggeyilng
      901 nfmvmpqkii irpgltieys gpvspverln tsepidtdli levlsvfnly ppqityeytv
      961 pkhilhsytw ilsdwsecth tcqgmkyrka ecrstenkdv vpddychnte klreesqicn
     1021 nhctlkweit svsecssqcg pgtrtvtsrc lqklinsdhp prpiptraca hlprpsekep
     1081 cigpcanvhw nyskwstcsv tcgggvqyrt avcvdsnwrr vsdescarqe khlkricgqd
     1141 pcpqwdlgkw hscsvtcgig krhrdylchv enrivphsyc gdpppgvvev cnagpceqwq
     1201 agiwspcsvt cgegikrrkv ickhsdrsis dkcppsekpe dtticilkpc ptvvneppik
     1261 yssdpphgih sqqnnevydi tfrsgykwhg sqkecsrpci ggymhtmvkc isieteiiap
     1321 dhycdrkekp ptmipcnrhr cpiwntgdws qcdvecgtgf qhrqvrcqsp rgeiladerc
     1381 hdsekpeivk tcrkspcitg ptdrknrlet nifrkwkvss wtpcskscgs glqrrrvect
     1441 mrrgnhgpev tvkdeqcsrl glskprsqrp crrvacdyiw qegpwsecsa qcgegiqsra
     1501 vtchranhhg midptptdnc pmdqkpssqq tcklrecddk yywttgqwtk cshtcgqkgr
     1561 qtrklfchde tgkkvarvnc prefkpqrkr kcnqrkcypm scleakkyyk ttkdseypli
     1621 iggrsmsiyc hemstsepke yltlrssfwe nyaevydksl lhphtcpyng erndscncat
     1681 vngtisgkti yrriridpvk lyvidndytf swtngtnpvk ygtagdcysq aecpqgrfsi
     1741 nlsgtqfkls sdvvwprrmg svveinkins qrvlgkcggy cgycspkpgl kldvlpp
//
LOCUS       XP_076546513            1735 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 9
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546513
VERSION     XP_076546513.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690398.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1735
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 9 isoform X3"
                     /calculated_mol_wt=196988
     Region          132..253
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          380..590
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(527..528,531,537)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          606..673
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          685..737
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          741..844
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          846..963
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          972..1024
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1029..1087
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1092..1144
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1147..1198
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1201..1252
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1296..1343
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1346..1399
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1418..1475
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1481..1535
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1537..1732
                     /region_name="GON"
                     /note="GON domain; pfam08685"
                     /db_xref="CDD:462559"
     CDS             1..1735
                     /gene="LOC117607743"
                     /coded_by="XM_076690398.1:746..5953"
                     /db_xref="GeneID:117607743"
ORIGIN      
        1 mcsagrgvvd msrisnrast iaailsilll vvflvvlwtg irtsnsrasg ietfdgfwke
       61 ilnetttgra vfdeesfsne vipdikktsr eqndisndas smhpdleyvs lkkisenqye
      121 dppdefstak rhhsghfrhr taevwdphpq yeftafgrrf rlrlahdtsf vspnirithm
      181 tengtrkehp ghelgcfysg tvdddpssvv svslchgmtg hvrtstgsyi ikpveswqen
      241 dkdprefslq haiqrirspn adhpnndekk srthscavid ddtptpildd mdgsqiyvgg
      301 rtrrrrslte ktaldhsqin esyeqfignn eehrnfirnd ryysverrrn easeeysqds
      361 dmesdpfvtw rprralprey fieimvaada emvryhgkgl fsyilvlmsm vsriykdrsi
      421 gnpinisvmk iiptdkmfga rhtvsegiaa admlerfchw qknnnpdeps rehhdlalll
      481 trenlchpee sscdtlglak lgrmcspiss caivqdngla avftiaheig hvfnmphdde
      541 akcegfrnhs gfhhvmsrml naqtfpwews ncsrhyvtef leagygncll depditmege
      601 dvsrlpgedf sgnkqcelvf gpgswicsdm vsdvcktlwc hvphsvrcrt syspwadgtv
      661 cgenrwchrg kcvsrinlqp vdgqwgewgr ygecsrtcgg gikkkyrecn npppqnygky
      721 cvgervkyrs catkecpgnh dfreqqcsqf dnnnlnilnl tkdvkwhaky rgilpperck
      781 lycqvasnqy ymlrekvidg tpcgrdtfhv cvnghckaag cdhvlnstae ldicgvckgd
      841 nstcqritgs ynstefgytr vakipagskd idirqhgwlg mgddnnylal rlgeggeyil
      901 ngnfmvmpqk iiirpgltie ysgpvspver lntsepidtd lilevlsvfn lyppqityey
      961 tvpkhilhsy twilsdwsec thtcqgmkyr kaecrstenk dvvpddychn teklreesqi
     1021 cnnhctlkwe itsvsecssq cgpgtrtvts rclqklinsd hpprpiptra cahlprpsek
     1081 epcigpcanv hwnyskwstc svtcgggvqy rtavcvdsnw rrvsdescar qekhlkricg
     1141 qdpcpqwdlg kwhscsvtcg igkrhrdylc hvenrivphs ycgdpppgvv evcnagpceq
     1201 wqagiwspcs vtcgegikrr kvickhsdrs isdkcppsek pedtticilk pcptvvnepp
     1261 ikyssdpphg ihsqqnnevy ditfrsgykw hgsqkecsrp ciggymhtmv kcisieteii
     1321 apdhycdrke kpptmipcnr hrcpiwntgd wsqcdvecgt gfqhrqvrcq sprgeilade
     1381 rchdsekpei vktcrkspci tgptdrknrl etnifrkwkv sswtpcsaqc gegiqsravt
     1441 chranhhgmi dptptdncpm dqkpssqqtc klrecddkyy wttgqwtkcs htcgqkgrqt
     1501 rklfchdetg kkvarvncpr efkpqrkrkc nqrkcypmsc leakkyyktt kdseypliig
     1561 grsmsiyche mstsepkeyl tlrssfweny aevydksllh phtcpynger ndscncatvn
     1621 gtisgktiyr riridpvkly vidndytfsw tngtnpvkyg tagdcysqae cpqgrfsinl
     1681 sgtqfklssd vvwprrmgsv veinkinsqr vlgkcggycg ycspkpglkl dvlpp
//
LOCUS       XP_034187734             318 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607759 [Osmia lignaria lignaria].
ACCESSION   XP_034187734
VERSION     XP_034187734.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331843.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135040) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187734.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="9"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="uncharacterized protein LOC117607759"
                     /calculated_mol_wt=38021
     CDS             1..318
                     /gene="LOC117607759"
                     /coded_by="XM_034331843.2:270..1226"
                     /db_xref="GeneID:117607759"
ORIGIN      
        1 meeclrlntk sdisgediqi tlnvlknhin eyevkkcmis kedvtrsqri eelvtkekrl
       61 sqqfhqirre irryerryek ilkkkvklms mqikddlyrl ntlkekyqel neqlnksyhe
      121 tnketkehtk krckvrkikk glrmfkqkiv pfsrkikiki kqnkklgrre mlfvndsntq
      181 qskqnkklgr remlfdndsn tqqskqnkkl srremlfvnd sntqqsftkv vnvpksnrln
      241 treckvvlkk lseerinkii gqgenqqknk qtsvnssqkn nesettdkpn whikipksvf
      301 eeiqkedrgt vlndnisy
//
LOCUS       XP_034192328            1601 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192328
VERSION     XP_034192328.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192328.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1601
                     /product="uncharacterized protein LOC117609763 isoform X1"
                     /calculated_mol_wt=189063
     Region          <341..1162
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          890..>1117
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <929..1431
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     CDS             1..1601
                     /gene="LOC117609763"
                     /coded_by="XM_034336437.2:205..5010"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 msyaehgpri flankynagt pliygdyvlp kkrlegrlkl ydscsdiraw spnylensry
       61 frelnengsp igkiipfhvv idniihlnsq aqnelqivke elqkykclkl spkqeqqlhs
      121 fenkeemkkr lqndncttsf plrdafvdis ksvatinsns clsihipykg hfqnnikdes
      181 tlrnrsnevc pysltaydss lnnsnlvpsf rciksflted fsekplpcik cidvavqvem
      241 emcnagkids qmakyfnkss tksvmsqtsf aykysdedns mtetgssslq tnndkehsfe
      301 cdascsessq nsettiefct emqaesnldl hqrihkgiii qteskiiale helcvrdadl
      361 kelqdvkndl ekslqekedy iktqqenlkl lydrllklhq qhnyeienlk qklrdykyli
      421 eqlqqdlsnk yetchlqsqe ieklklcvkd vamlqlekts yieklqemeq svkkaktcnl
      481 alqqiksilq eknelkkeny eqchilidqe eeinqlinei siayeeqrkt kdilenlkae
      541 ihnkdikisq yekellsmkq eisdffnnlk halnnleeln glcedvynct nceldineea
      601 nnvlqninti mtrfqsykie sqnlvqeien lkrciennkq qihldqnfrn iidedfyyep
      661 dinlkntsgk dnmivlnikq dksstneiel eevsnsvnsk nslcttshnl diykhktech
      721 nyiikldnkt nidkvekeii eyfsqslikl hsltqklkiy veterpfvik liyklheile
      781 diqsavnisq ditlrklris klysqhkeey vkynscveev ptdlfkiqnr inefieaily
      841 qllneilrie qeslhnrkin cafemyfksc idrvnkvvqg agdqriqivd viekqqrelq
      901 ekdteiaqlk keiaehlerr rgegdclnqq efeksiaikq elskviteln nknaliselk
      961 gqlqifknel krknknlyni knklwkecqq trkrltmknl eiknlykklh nlskindlnn
     1021 amenelskmk kelsdiqlkn siileeqkee irkrntiisq kdetivilkn rleknetiss
     1081 reigeheivv kekedeivkl qnenkllgek lkdneskfir mnqtitslte qrdkiniiyv
     1141 vqedlakldk nkeklkveln slkvqlvndq nnnkkldtdl giilheneil qkyveywkne
     1201 nseltmklcn kiseeklksq lyalsnnilq kleilkkqct seenlanlqf sehslnkcti
     1261 hqdeimpltt nyinrdfklk tnnqereike neieevelrq llntanitkn inttsctmfk
     1321 qfessennnr kmkyktisfe nvhyedqnei enslneieir dckirnytet ieqltqenmd
     1381 lravlksqae yqnkltlmkk nydsslkavn erhkknieil qtqfednlks erifdsenwl
     1441 qslnmkelme lnerisviin snsnlmcakn knqcfyendv hkefytkide ieekiemrpk
     1501 niygreqtnt rlekqwplti fndiqnyptl qrqddklsle hsiryrycee kpselenkye
     1561 kekqvekdst fdqqrwnfik qcsayhklst tyeysrtiht d
//
LOCUS       XP_076546514            1583 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546514
VERSION     XP_076546514.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690399.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1583
                     /product="uncharacterized protein LOC117609763 isoform X2"
                     /calculated_mol_wt=186846
     Region          <341..1162
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          890..>1117
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <929..1431
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     CDS             1..1583
                     /gene="LOC117609763"
                     /coded_by="XM_076690399.1:206..4957"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 msyaehgpri flankynagt pliygdyvlp kkrlegrlkl ydscsdiraw spnylensry
       61 frelnengsp igkiipfhvv idniihlnsq aqnelqivke elqkykclkl spkqeqqlhs
      121 fenkeemkkr lqndncttsf plrdafvdis ksvatinsns clsihipykg hfqnnikdes
      181 tlrnrsnevc pysltaydss lnnsnlvpsf rciksflted fsekplpcik cidvavqvem
      241 emcnagkids qmakyfnkss tksvmsqtsf aykysdedns mtetgssslq tnndkehsfe
      301 cdascsessq nsettiefct emqaesnldl hqrihkgiii qteskiiale helcvrdadl
      361 kelqdvkndl ekslqekedy iktqqenlkl lydrllklhq qhnyeienlk qklrdykyli
      421 eqlqqdlsnk yetchlqsqe ieklklcvkd vamlqlekts yieklqemeq svkkaktcnl
      481 alqqiksilq eknelkkeny eqchilidqe eeinqlinei siayeeqrkt kdilenlkae
      541 ihnkdikisq yekellsmkq eisdffnnlk halnnleeln glcedvynct nceldineea
      601 nnvlqninti mtrfqsykie sqnlvqeien lkrciennkq qihldqnfrn iidedfyyep
      661 dinlkntsgk dnmivlnikq dksstneiel eevsnsvnsk nslcttshnl diykhktech
      721 nyiikldnkt nidkvekeii eyfsqslikl hsltqklkiy veterpfvik liyklheile
      781 diqsavnisq ditlrklris klysqhkeey vkynscveev ptdlfkiqnr inefieaily
      841 qllneilrie qeslhnrkin cafemyfksc idrvnkvvqg agdqriqivd viekqqrelq
      901 ekdteiaqlk keiaehlerr rgegdclnqq efeksiaikq elskviteln nknaliselk
      961 gqlqifknel krknknlyni knklwkecqq trkrltmknl eiknlykklh nlskindlnn
     1021 amenelskmk kelsdiqlkn siileeqkee irkrntiisq kdetivilkn rleknetiss
     1081 reigeheivv kekedeivkl qnenkllgek lkdneskfir mnqtitslte qrdkiniiyv
     1141 vqedlakldk nkeklkveln slkvqlvndq nnnkkldtdl giilheneil qkyveywkne
     1201 nseltmklcn kiseeklksq lyalsnnilq kleilkkqct seenlanlqf sehslnkcti
     1261 hqdeimpltt nyinrdfklk tnnqereike neieevelrq llntanitkn inttsctmfk
     1321 qfessennnr kmkyktisfe nvhyedqnei enslneieir dckirnytet ieqltqenmd
     1381 lravlksqae yqnkltlmkk nydsslkavn erhkknieil qtqfednlks erifdsenwl
     1441 qslnmkelme lnerisviin snsnlmcakn knqcfyendv hkefytkide ieekiemrpk
     1501 niygreqtnt rlekqwplti fndiqnyptl qrqddklsle hsiryrycee kpselenkye
     1561 kekqvekdst eqchklqete hlr
//
LOCUS       XP_034192336            1582 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192336
VERSION     XP_034192336.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336445.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192336.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1582
                     /product="uncharacterized protein LOC117609763 isoform X3"
                     /calculated_mol_wt=186977
     Region          <322..1143
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          871..>1098
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <910..1412
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     CDS             1..1582
                     /gene="LOC117609763"
                     /coded_by="XM_034336445.2:205..4953"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 msyaehgpri flankynagt pliygdyvlp kkrlegrlkl ydscsdiraw spnylensry
       61 frelnengsp igkiipfhvv idniihlnsq aqnelqivke elqkykclkl spkqeqqlhs
      121 fenkeemkkr lqndncttsf plrdafvdis ksvatinsns clsihipykg hfqnnikdes
      181 tlrnrsnevc pysltaydss lnnsnlvpsf rciksfltee memcnagkid sqmakyfnks
      241 stksvmsqts faykysdedn smtetgsssl qtnndkehsf ecdascsess qnsettiefc
      301 temqaesnld lhqrihkgii iqteskiial ehelcvrdad lkelqdvknd lekslqeked
      361 yiktqqenlk llydrllklh qqhnyeienl kqklrdykyl ieqlqqdlsn kyetchlqsq
      421 eieklklcvk dvamlqlekt syieklqeme qsvkkaktcn lalqqiksil qeknelkken
      481 yeqchilidq eeeinqline isiayeeqrk tkdilenlka eihnkdikis qyekellsmk
      541 qeisdffnnl khalnnleel nglcedvync tnceldinee annvlqnint imtrfqsyki
      601 esqnlvqeie nlkrciennk qqihldqnfr niidedfyye pdinlkntsg kdnmivlnik
      661 qdksstneie leevsnsvns knslcttshn ldiykhktec hnyiikldnk tnidkvekei
      721 ieyfsqslik lhsltqklki yveterpfvi kliyklheil ediqsavnis qditlrklri
      781 sklysqhkee yvkynscvee vptdlfkiqn rinefieail yqllneilri eqeslhnrki
      841 ncafemyfks cidrvnkvvq gagdqriqiv dviekqqrel qekdteiaql kkeiaehler
      901 rrgegdclnq qefeksiaik qelskvitel nnknalisel kgqlqifkne lkrknknlyn
      961 iknklwkecq qtrkrltmkn leiknlykkl hnlskindln namenelskm kkelsdiqlk
     1021 nsiileeqke eirkrntiis qkdetivilk nrleknetis sreigeheiv vkekedeivk
     1081 lqnenkllge klkdneskfi rmnqtitslt eqrdkiniiy vvqedlakld knkeklkvel
     1141 nslkvqlvnd qnnnkkldtd lgiilhenei lqkyveywkn enseltmklc nkiseeklks
     1201 qlyalsnnil qkleilkkqc tseenlanlq fsehslnkct ihqdeimplt tnyinrdfkl
     1261 ktnnqereik eneieevelr qllntanitk ninttsctmf kqfessennn rkmkyktisf
     1321 envhyedqne ienslneiei rdckirnyte tieqltqenm dlravlksqa eyqnkltlmk
     1381 knydsslkav nerhkkniei lqtqfednlk serifdsenw lqslnmkelm elnerisvii
     1441 nsnsnlmcak nknqcfyend vhkefytkid eieekiemrp kniygreqtn trlekqwplt
     1501 ifndiqnypt lqrqddklsl ehsiryryce ekpselenky ekekqvekds tfdqqrwnfi
     1561 kqcsayhkls ttyeysrtih td
//
LOCUS       XP_034192340            1580 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192340
VERSION     XP_034192340.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336449.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192340.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1580
                     /product="uncharacterized protein LOC117609763 isoform X4"
                     /calculated_mol_wt=186640
     Region          <320..1141
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          869..>1096
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <908..1410
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     CDS             1..1580
                     /gene="LOC117609763"
                     /coded_by="XM_034336449.2:204..4946"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 msyaehgpri flankynagt pliydiraws pnylensryf relnengspi gkiipfhvvi
       61 dniihlnsqa qnelqivkee lqkykclkls pkqeqqlhsf enkeemkkrl qndncttsfp
      121 lrdafvdisk svatinsnsc lsihipykgh fqnnikdest lrnrsnevcp ysltaydssl
      181 nnsnlvpsfr ciksfltedf sekplpcikc idvavqveme mcnagkidsq makyfnksst
      241 ksvmsqtsfa ykysdednsm tetgssslqt nndkehsfec dascsessqn settiefcte
      301 mqaesnldlh qrihkgiiiq teskiialeh elcvrdadlk elqdvkndle kslqekedyi
      361 ktqqenlkll ydrllklhqq hnyeienlkq klrdykylie qlqqdlsnky etchlqsqei
      421 eklklcvkdv amlqlektsy ieklqemeqs vkkaktcnla lqqiksilqe knelkkenye
      481 qchilidqee einqlineis iayeeqrktk dilenlkaei hnkdikisqy ekellsmkqe
      541 isdffnnlkh alnnleelng lcedvynctn celdineean nvlqnintim trfqsykies
      601 qnlvqeienl krciennkqq ihldqnfrni idedfyyepd inlkntsgkd nmivlnikqd
      661 ksstneiele evsnsvnskn slcttshnld iykhktechn yiikldnktn idkvekeiie
      721 yfsqsliklh sltqklkiyv eterpfvikl iyklheiled iqsavnisqd itlrklrisk
      781 lysqhkeeyv kynscveevp tdlfkiqnri nefieailyq llneilrieq eslhnrkinc
      841 afemyfksci drvnkvvqga gdqriqivdv iekqqrelqe kdteiaqlkk eiaehlerrr
      901 gegdclnqqe feksiaikqe lskvitelnn knaliselkg qlqifknelk rknknlynik
      961 nklwkecqqt rkrltmknle iknlykklhn lskindlnna menelskmkk elsdiqlkns
     1021 iileeqkeei rkrntiisqk detivilknr leknetissr eigeheivvk ekedeivklq
     1081 nenkllgekl kdneskfirm nqtitslteq rdkiniiyvv qedlakldkn keklkvelns
     1141 lkvqlvndqn nnkkldtdlg iilheneilq kyveywknen seltmklcnk iseeklksql
     1201 yalsnnilqk leilkkqcts eenlanlqfs ehslnkctih qdeimplttn yinrdfklkt
     1261 nnqereiken eieevelrql lntanitkni nttsctmfkq fessennnrk mkyktisfen
     1321 vhyedqneie nslneieird ckirnyteti eqltqenmdl ravlksqaey qnkltlmkkn
     1381 ydsslkavne rhkknieilq tqfednlkse rifdsenwlq slnmkelmel nerisviins
     1441 nsnlmcaknk nqcfyendvh kefytkidei eekiemrpkn iygreqtntr lekqwpltif
     1501 ndiqnyptlq rqddklsleh siryryceek pselenkyek ekqvekdstf dqqrwnfikq
     1561 csayhklstt yeysrtihtd
//
LOCUS       XP_034192347            1551 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192347
VERSION     XP_034192347.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336456.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192347.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1551
                     /product="uncharacterized protein LOC117609763 isoform X5"
                     /calculated_mol_wt=183046
     Region          357..1158
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          840..>1067
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     CDS             1..1551
                     /gene="LOC117609763"
                     /coded_by="XM_034336456.2:207..4862"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 msyaehgpri flankynagt pliygdyvlp kkrlegrlkl ydscsdiraw spnylensry
       61 frelnengsp igkiipfhvv idniihlnsq aqnelqivke elqkykclkl spkqeqqlhs
      121 fenkeemkkr lqndncttsf plrdafvdis ksvatinsns clsihipykg hfqnnikdes
      181 tlrnrsnevc pysltaydss lnnsnlvpsf rciksflted fsekplpcik cidvavqvem
      241 emcnagkids qmakyfnkss tksvmsqtsf aykysdedns mtetgssslq tnndkehsfe
      301 cdascsessq nsettiefct emqaesnldl hqrihkgiii qteskiiale helcvrdadl
      361 kelqdvkndl ekslqekedy iktqqenlkl lydrllklhq qhnyeienlk qkeklqemeq
      421 svkkaktcnl alqqiksilq eknelkkeny eqchilidqe eeinqlinei siayeeqrkt
      481 kdilenlkae ihnkdikisq yekellsmkq eisdffnnlk halnnleeln glcedvynct
      541 nceldineea nnvlqninti mtrfqsykie sqnlvqeien lkrciennkq qihldqnfrn
      601 iidedfyyep dinlkntsgk dnmivlnikq dksstneiel eevsnsvnsk nslcttshnl
      661 diykhktech nyiikldnkt nidkvekeii eyfsqslikl hsltqklkiy veterpfvik
      721 liyklheile diqsavnisq ditlrklris klysqhkeey vkynscveev ptdlfkiqnr
      781 inefieaily qllneilrie qeslhnrkin cafemyfksc idrvnkvvqg agdqriqivd
      841 viekqqrelq ekdteiaqlk keiaehlerr rgegdclnqq efeksiaikq elskviteln
      901 nknaliselk gqlqifknel krknknlyni knklwkecqq trkrltmknl eiknlykklh
      961 nlskindlnn amenelskmk kelsdiqlkn siileeqkee irkrntiisq kdetivilkn
     1021 rleknetiss reigeheivv kekedeivkl qnenkllgek lkdneskfir mnqtitslte
     1081 qrdkiniiyv vqedlakldk nkeklkveln slkvqlvndq nnnkkldtdl giilheneil
     1141 qkyveywkne nseltmklcn kiseeklksq lyalsnnilq kleilkkqct seenlanlqf
     1201 sehslnkcti hqdeimpltt nyinrdfklk tnnqereike neieevelrq llntanitkn
     1261 inttsctmfk qfessennnr kmkyktisfe nvhyedqnei enslneieir dckirnytet
     1321 ieqltqenmd lravlksqae yqnkltlmkk nydsslkavn erhkknieil qtqfednlks
     1381 erifdsenwl qslnmkelme lnerisviin snsnlmcakn knqcfyendv hkefytkide
     1441 ieekiemrpk niygreqtnt rlekqwplti fndiqnyptl qrqddklsle hsiryrycee
     1501 kpselenkye kekqvekdst fdqqrwnfik qcsayhklst tyeysrtiht d
//
LOCUS       XP_076546515            1509 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546515
VERSION     XP_076546515.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690400.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1509
                     /product="uncharacterized protein LOC117609763 isoform X6"
                     /calculated_mol_wt=177612
     Region          <341..1162
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          890..>1117
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <929..1431
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     CDS             1..1509
                     /gene="LOC117609763"
                     /coded_by="XM_076690400.1:207..4736"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 msyaehgpri flankynagt pliygdyvlp kkrlegrlkl ydscsdiraw spnylensry
       61 frelnengsp igkiipfhvv idniihlnsq aqnelqivke elqkykclkl spkqeqqlhs
      121 fenkeemkkr lqndncttsf plrdafvdis ksvatinsns clsihipykg hfqnnikdes
      181 tlrnrsnevc pysltaydss lnnsnlvpsf rciksflted fsekplpcik cidvavqvem
      241 emcnagkids qmakyfnkss tksvmsqtsf aykysdedns mtetgssslq tnndkehsfe
      301 cdascsessq nsettiefct emqaesnldl hqrihkgiii qteskiiale helcvrdadl
      361 kelqdvkndl ekslqekedy iktqqenlkl lydrllklhq qhnyeienlk qklrdykyli
      421 eqlqqdlsnk yetchlqsqe ieklklcvkd vamlqlekts yieklqemeq svkkaktcnl
      481 alqqiksilq eknelkkeny eqchilidqe eeinqlinei siayeeqrkt kdilenlkae
      541 ihnkdikisq yekellsmkq eisdffnnlk halnnleeln glcedvynct nceldineea
      601 nnvlqninti mtrfqsykie sqnlvqeien lkrciennkq qihldqnfrn iidedfyyep
      661 dinlkntsgk dnmivlnikq dksstneiel eevsnsvnsk nslcttshnl diykhktech
      721 nyiikldnkt nidkvekeii eyfsqslikl hsltqklkiy veterpfvik liyklheile
      781 diqsavnisq ditlrklris klysqhkeey vkynscveev ptdlfkiqnr inefieaily
      841 qllneilrie qeslhnrkin cafemyfksc idrvnkvvqg agdqriqivd viekqqrelq
      901 ekdteiaqlk keiaehlerr rgegdclnqq efeksiaikq elskviteln nknaliselk
      961 gqlqifknel krknknlyni knklwkecqq trkrltmknl eiknlykklh nlskindlnn
     1021 amenelskmk kelsdiqlkn siileeqkee irkrntiisq kdetivilkn rleknetiss
     1081 reigeheivv kekedeivkl qnenkllgek lkdneskfir mnqtitslte qrdkiniiyv
     1141 vqedlakldk nkeklkveln slkvqlvndq nnnkkldtdl giilheneil qkyveywkne
     1201 nseltmklcn kiseeklksq lyalsnnilq kleilkkqct seenlanlqf sehslnkcti
     1261 hqdeimpltt nyinrdfklk tnnqereike neieevelrq llntanitkn inttsctmfk
     1321 qfessennnr kmkyktisfe nvhyedqnei enslneieir dckirnytet ieqltqenmd
     1381 lravlksqae yqnkltlmkk nydsslkavn erhkknieil qtqfednlks erifdsenwl
     1441 qslnmkelme lnerisviin snsnlmcakn knqcfyendv hkefytkide ieekiemrpk
     1501 niygrgtmp
//
LOCUS       XP_034192360            1485 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192360
VERSION     XP_034192360.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336469.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192360.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1485
                     /product="uncharacterized protein LOC117609763 isoform X7"
                     /calculated_mol_wt=175913
     Region          234..1046
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          774..>1001
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <813..1315
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     CDS             1..1485
                     /gene="LOC117609763"
                     /coded_by="XM_034336469.2:204..4661"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 msyaehgpri flankynagt pliygdyvlp kkrlegrlkl ydscsdiraw spnylensry
       61 frelnengsp igkiipfhvv idniihlnsq aqnelqivke elqkykclkl spkqeqqlhs
      121 fvememcnag kidsqmakyf nksstksvms qtsfaykysd ednsmtetgs sslqtnndke
      181 hsfecdascs essqnsetti efctemqaes nldlhqrihk giiiqteski ialehelcvr
      241 dadlkelqdv kndlekslqe kedyiktqqe nlkllydrll klhqqhnyei enlkqklrdy
      301 kylieqlqqd lsnkyetchl qsqeieklkl cvkdvamlql ektsyieklq emeqsvkkak
      361 tcnlalqqik silqeknelk kenyeqchil idqeeeinql ineisiayee qrktkdilen
      421 lkaeihnkdi kisqyekell smkqeisdff nnlkhalnnl eelnglcedv ynctnceldi
      481 neeannvlqn intimtrfqs ykiesqnlvq eienlkrcie nnkqqihldq nfrniidedf
      541 yyepdinlkn tsgkdnmivl nikqdksstn eieleevsns vnsknslctt shnldiykhk
      601 technyiikl dnktnidkve keiieyfsqs liklhsltqk lkiyveterp fvikliyklh
      661 eilediqsav nisqditlrk lrisklysqh keeyvkynsc veevptdlfk iqnrinefie
      721 ailyqllnei lrieqeslhn rkincafemy fkscidrvnk vvqgagdqri qivdviekqq
      781 relqekdtei aqlkkeiaeh lerrrgegdc lnqqefeksi aikqelskvi telnnknali
      841 selkgqlqif knelkrknkn lyniknklwk ecqqtrkrlt mknleiknly kklhnlskin
      901 dlnnamenel skmkkelsdi qlknsiilee qkeeirkrnt iisqkdetiv ilknrlekne
      961 tissreigeh eivvkekede ivklqnenkl lgeklkdnes kfirmnqtit slteqrdkin
     1021 iiyvvqedla kldknkeklk velnslkvql vndqnnnkkl dtdlgiilhe neilqkyvey
     1081 wknenseltm klcnkiseek lksqlyalsn nilqkleilk kqctseenla nlqfsehsln
     1141 kctihqdeim plttnyinrd fklktnnqer eikeneieev elrqllntan itkninttsc
     1201 tmfkqfesse nnnrkmkykt isfenvhyed qneienslne ieirdckirn ytetieqltq
     1261 enmdlravlk sqaeyqnklt lmkknydssl kavnerhkkn ieilqtqfed nlkserifds
     1321 enwlqslnmk elmelneris viinsnsnlm caknknqcfy endvhkefyt kideieekie
     1381 mrpkniygre qtntrlekqw pltifndiqn yptlqrqddk lslehsiryr yceekpsele
     1441 nkyekekqve kdstfdqqrw nfikqcsayh klsttyeysr tihtd
//
LOCUS       XP_034192363            1362 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609763 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192363
VERSION     XP_034192363.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336472.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192363.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1362
                     /product="uncharacterized protein LOC117609763 isoform X8"
                     /calculated_mol_wt=161579
     Region          111..923
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          651..>878
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          <690..1192
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     CDS             1..1362
                     /gene="LOC117609763"
                     /coded_by="XM_034336472.2:146..4234"
                     /db_xref="GeneID:117609763"
ORIGIN      
        1 memcnagkid sqmakyfnks stksvmsqts faykysdedn smtetgsssl qtnndkehsf
       61 ecdascsess qnsettiefc temqaesnld lhqrihkgii iqteskiial ehelcvrdad
      121 lkelqdvknd lekslqeked yiktqqenlk llydrllklh qqhnyeienl kqklrdykyl
      181 ieqlqqdlsn kyetchlqsq eieklklcvk dvamlqlekt syieklqeme qsvkkaktcn
      241 lalqqiksil qeknelkken yeqchilidq eeeinqline isiayeeqrk tkdilenlka
      301 eihnkdikis qyekellsmk qeisdffnnl khalnnleel nglcedvync tnceldinee
      361 annvlqnint imtrfqsyki esqnlvqeie nlkrciennk qqihldqnfr niidedfyye
      421 pdinlkntsg kdnmivlnik qdksstneie leevsnsvns knslcttshn ldiykhktec
      481 hnyiikldnk tnidkvekei ieyfsqslik lhsltqklki yveterpfvi kliyklheil
      541 ediqsavnis qditlrklri sklysqhkee yvkynscvee vptdlfkiqn rinefieail
      601 yqllneilri eqeslhnrki ncafemyfks cidrvnkvvq gagdqriqiv dviekqqrel
      661 qekdteiaql kkeiaehler rrgegdclnq qefeksiaik qelskvitel nnknalisel
      721 kgqlqifkne lkrknknlyn iknklwkecq qtrkrltmkn leiknlykkl hnlskindln
      781 namenelskm kkelsdiqlk nsiileeqke eirkrntiis qkdetivilk nrleknetis
      841 sreigeheiv vkekedeivk lqnenkllge klkdneskfi rmnqtitslt eqrdkiniiy
      901 vvqedlakld knkeklkvel nslkvqlvnd qnnnkkldtd lgiilhenei lqkyveywkn
      961 enseltmklc nkiseeklks qlyalsnnil qkleilkkqc tseenlanlq fsehslnkct
     1021 ihqdeimplt tnyinrdfkl ktnnqereik eneieevelr qllntanitk ninttsctmf
     1081 kqfessennn rkmkyktisf envhyedqne ienslneiei rdckirnyte tieqltqenm
     1141 dlravlksqa eyqnkltlmk knydsslkav nerhkkniei lqtqfednlk serifdsenw
     1201 lqslnmkelm elnerisvii nsnsnlmcak nknqcfyend vhkefytkid eieekiemrp
     1261 kniygreqtn trlekqwplt ifndiqnypt lqrqddklsl ehsiryryce ekpselenky
     1321 ekekqvekds tfdqqrwnfi kqcsayhkls ttyeysrtih td
//
LOCUS       XP_034192367            1184 aa            linear   INV 03-SEP-2025
DEFINITION  haspin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192367
VERSION     XP_034192367.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336476.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192367.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1184
                     /product="haspin isoform X1"
                     /calculated_mol_wt=135275
     Region          842..1136
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(871..874,877,879,890,892,956,985..988,1029,
                     1033..1034,1036,1066..1067)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1184
                     /gene="Haspin"
                     /coded_by="XM_034336476.2:610..4164"
                     /db_xref="GeneID:117609797"
ORIGIN      
        1 mnpqfskpir tyerkkckdv aviypnlhiq ssydvdsknp ilfkkklinv erkeanddsm
       61 sydpfdttfd rlarntripp pvaktcnnen synnsatsts wndnesenks iewsdvtdkk
      121 lisvqkivhe kpkmqvvkkk ikrnkikskv kksdtykryk lcktkdnnks vsthlnidsl
      181 nlpseidsei rtqgknykky tlgkstkdig glnkqsteni qmkscsvvln tlstircdnk
      241 vnsmendlsl gvhentskkq rlskqditkv nlknvecndi dvrycfvklk qldvdsnakf
      301 fnfgkkcgri fsstpksrsv kshiislspi glsnklnnsi mlednistta dnkdsvsnns
      361 nevnaclhak sdlpstdvtn itnlskcnlk llknsltifd cndnnvcdqk ehstegtcek
      421 slklvrnnsi neikeihvsn klaqecvvvd tekrkyqlnf sidcnrshsl fdddtdenig
      481 nklhidvavs nkmstyntml nktnstsdne iivdinstdi kvtcdkikei sselsdlsks
      541 vildasldvk mietngglnk enaskqiesl pkkndtsitd ksfvnndknh svhdsyvilk
      601 rlqdpirvsk rrkqyckwnl dmtsifedsd nnterinler gkepkkqmls leklfsleni
      661 ieesdnkesv eikksiylkp gkswarslsi lnniqnisgl dklsigkgkr wrnsvqdvln
      721 mqkqgiiqsc ikenssgkel qvcneaines kcklddnksr tcdstnfghl srrisvcvvp
      781 ihktvesied apflevygiv pvksqrytll nnpkkssacn ihsdddftgc dikehvtsta
      841 kevilqkclq edyipfsayf tdsylehcrk igegvygevf lyaqedkksv ikvipiegne
      901 yvngepqkkf heilseiini frelhnlrfn tkynsdgfve vknikcikgk ypeklvelwn
      961 vydeekhsdn dcpsvfnddq lyivlelghg gqdleafvfn taeeahilfi qaalalavae
     1021 kavefehrdl hwgnilispt ndshvyyklg rrkikliskg vkvsiidftl srvtyqgcsv
     1081 fndlaldptl ftaqgeyqfe vyrlmrdkvk ndwqqfepyt nilwlhytld kmitavryrk
     1141 rnlrihqnni kklqelknei ltyssafdfv sncdkivnlv cnds
//
LOCUS       XP_034192374            1183 aa            linear   INV 03-SEP-2025
DEFINITION  haspin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192374
VERSION     XP_034192374.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336483.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192374.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1183
                     /product="haspin isoform X2"
                     /calculated_mol_wt=135046
     Region          842..1135
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(871..874,877,879,890,892,955,984..987,1028,
                     1032..1033,1035,1065..1066)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1183
                     /gene="Haspin"
                     /coded_by="XM_034336483.2:591..4142"
                     /db_xref="GeneID:117609797"
ORIGIN      
        1 mnpqfskpir tyerkkckdv aviypnlhiq ssydvdsknp ilfkkklinv erkeanddsm
       61 sydpfdttfd rlarntripp pvaktcnnen synnsatsts wndnesenks iewsdvtdkk
      121 lisvqkivhe kpkmqvvkkk ikrnkikskv kksdtykryk lcktkdnnks vsthlnidsl
      181 nlpseidsei rtqgknykky tlgkstkdig glnkqsteni qmkscsvvln tlstircdnk
      241 vnsmendlsl gvhentskkq rlskqditkv nlknvecndi dvrycfvklk qldvdsnakf
      301 fnfgkkcgri fsstpksrsv kshiislspi glsnklnnsi mlednistta dnkdsvsnns
      361 nevnaclhak sdlpstdvtn itnlskcnlk llknsltifd cndnnvcdqk ehstegtcek
      421 slklvrnnsi neikeihvsn klaqecvvvd tekrkyqlnf sidcnrshsl fdddtdenig
      481 nklhidvavs nkmstyntml nktnstsdne iivdinstdi kvtcdkikei sselsdlsks
      541 vildasldvk mietngglnk enaskqiesl pkkndtsitd ksfvnndknh svhdsyvilk
      601 rlqdpirvsk rrkqyckwnl dmtsifedsd nnterinler gkepkkqmls leklfsleni
      661 ieesdnkesv eikksiylkp gkswarslsi lnniqnisgl dklsigkgkr wrnsvqdvln
      721 mqkqgiiqsc ikenssgkel qvcneaines kcklddnksr tcdstnfghl srrisvcvvp
      781 ihktvesied apflevygiv pvksqrytll nnpkkssacn ihsdddftgc dikehvtsta
      841 kevilqkclq edyipfsayf tdsylehcrk igegvygevf lyaqedkksv ikvipiegne
      901 yvngepqkkf heilseivia melhnlrfnt kynsdgfvev knikcikgky peklvelwnv
      961 ydeekhsdnd cpsvfnddql yivlelghgg qdleafvfnt aeeahilfiq aalalavaek
     1021 avefehrdlh wgnilisptn dshvyyklgr rkikliskgv kvsiidftls rvtyqgcsvf
     1081 ndlaldptlf taqgeyqfev yrlmrdkvkn dwqqfepytn ilwlhytldk mitavryrkr
     1141 nlrihqnnik klqelkneil tyssafdfvs ncdkivnlvc nds
//
LOCUS       XP_034192383            1145 aa            linear   INV 03-SEP-2025
DEFINITION  haspin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034192383
VERSION     XP_034192383.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336492.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192383.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1145
                     /product="haspin isoform X3"
                     /calculated_mol_wt=130739
     Region          803..1097
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(832..835,838,840,851,853,917,946..949,990,994..995,
                     997,1027..1028)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1145
                     /gene="Haspin"
                     /coded_by="XM_034336492.2:643..4080"
                     /db_xref="GeneID:117609797"
ORIGIN      
        1 mkernkklin verkeandds msydpfdttf drlarntrip ppvaktcnne nsynnsatst
       61 swndnesenk siewsdvtdk klisvqkivh ekpkmqvvkk kikrnkiksk vkksdtykry
      121 klcktkdnnk svsthlnids lnlpseidse irtqgknykk ytlgkstkdi gglnkqsten
      181 iqmkscsvvl ntlstircdn kvnsmendls lgvhentskk qrlskqditk vnlknvecnd
      241 idvrycfvkl kqldvdsnak ffnfgkkcgr ifsstpksrs vkshiislsp iglsnklnns
      301 imlednistt adnkdsvsnn snevnaclha ksdlpstdvt nitnlskcnl kllknsltif
      361 dcndnnvcdq kehstegtce kslklvrnns ineikeihvs nklaqecvvv dtekrkyqln
      421 fsidcnrshs lfdddtdeni gnklhidvav snkmstyntm lnktnstsdn eiivdinstd
      481 ikvtcdkike isselsdlsk svildasldv kmietnggln kenaskqies lpkkndtsit
      541 dksfvnndkn hsvhdsyvil krlqdpirvs krrkqyckwn ldmtsifeds dnnterinle
      601 rgkepkkqml sleklfslen iieesdnkes veikksiylk pgkswarsls ilnniqnisg
      661 ldklsigkgk rwrnsvqdvl nmqkqgiiqs cikenssgke lqvcneaine skcklddnks
      721 rtcdstnfgh lsrrisvcvv pihktvesie dapflevygi vpvksqrytl lnnpkkssac
      781 nihsdddftg cdikehvtst akevilqkcl qedyipfsay ftdsylehcr kigegvygev
      841 flyaqedkks vikvipiegn eyvngepqkk fheilseiin ifrelhnlrf ntkynsdgfv
      901 evknikcikg kypeklvelw nvydeekhsd ndcpsvfndd qlyivlelgh ggqdleafvf
      961 ntaeeahilf iqaalalava ekavefehrd lhwgnilisp tndshvyykl grrkiklisk
     1021 gvkvsiidft lsrvtyqgcs vfndlaldpt lftaqgeyqf evyrlmrdkv kndwqqfepy
     1081 tnilwlhytl dkmitavryr krnlrihqnn ikklqelkne iltyssafdf vsncdkivnl
     1141 vcnds
//
LOCUS       XP_034192397             620 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger MYND domain-containing protein 11 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192397
VERSION     XP_034192397.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336506.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..620
                     /product="zinc finger MYND domain-containing protein 11
                     isoform X1"
                     /calculated_mol_wt=72157
     Region          93..>121
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(93,103..106,110)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          145..268
                     /region_name="Bromo_ZMYND11"
                     /note="Bromodomain; ZMYND11_like sub-family. ZMYND11 or
                     BS69 is a ubiquitously expressed nuclear protein that has
                     been shown to associate with chromatin. It interacts with
                     chromatin remodeling factors and might play a role in
                     chromatin remodeling and gene...; cd05492"
                     /db_xref="CDD:99924"
     Site            order(175,195,198,237,241,247)
                     /site_type="other"
                     /note="putative acetyllysine binding site [chemical
                     binding]"
                     /db_xref="CDD:99924"
     Region          286..381
                     /region_name="PWWP_BS69"
                     /note="PWWP domain found in protein BS69 and similar
                     proteins; cd20159"
                     /db_xref="CDD:438987"
     Site            order(288,292)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438987"
     Site            order(298,300,347,360..361,366..367)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438987"
     Site            order(302,305,321)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438987"
     Site            303..306
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438987"
     Region          581..616
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..620
                     /gene="LOC117609809"
                     /coded_by="XM_034336506.2:241..2103"
                     /db_xref="GeneID:117609809"
ORIGIN      
        1 msirrrtdpf mtqriwdaik itvhqrslps ndrmvrhlsr vygiteqeaq eelnkavddg
       61 lvylkrvptk sgieqesyrl padtvsnddh dwycckcqka gvvecceqch rvyhpechvp
      121 nniklkmchf cesinndtyq dkidlnhils ftcghlkakl ppeitnrtiv fnhdpivtan
      181 nkysgptwis egedawrpnv likhhmdlai mdskiknney nnlaefrada hnilhniiiy
      241 hgahsivgem gltmyqdccy dlqeirrcad cyrisnekse kmwfcipcnp phqlvyakqk
      301 gypywpakvm qvngniydvr ffgghhmran iekvfirpit sslqslqpse kgkwkemkik
      361 rstawnrafe elkhhqnlll klgkyindld inndsdgpkp tkirnieshp knstkqllkd
      421 lrvkverlss dshndilptq eatdnnerst ikakseerqv pclpvaedep argisstcpq
      481 glkkegsqed mvtsssqepr tkcvlvqteq iqtetlpaki krerrtseqp tttaveklrr
      541 elelekckel eklqaehake lrqltdrhqq iiseikkkqw cynceaeaiy hccwntaycs
      601 tdcqqvhwqr ehkrvcrrkr
//
LOCUS       XP_034192407             620 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger MYND domain-containing protein 11 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192407
VERSION     XP_034192407.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336516.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..620
                     /product="zinc finger MYND domain-containing protein 11
                     isoform X1"
                     /calculated_mol_wt=72157
     Region          93..>121
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(93,103..106,110)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          145..268
                     /region_name="Bromo_ZMYND11"
                     /note="Bromodomain; ZMYND11_like sub-family. ZMYND11 or
                     BS69 is a ubiquitously expressed nuclear protein that has
                     been shown to associate with chromatin. It interacts with
                     chromatin remodeling factors and might play a role in
                     chromatin remodeling and gene...; cd05492"
                     /db_xref="CDD:99924"
     Site            order(175,195,198,237,241,247)
                     /site_type="other"
                     /note="putative acetyllysine binding site [chemical
                     binding]"
                     /db_xref="CDD:99924"
     Region          286..381
                     /region_name="PWWP_BS69"
                     /note="PWWP domain found in protein BS69 and similar
                     proteins; cd20159"
                     /db_xref="CDD:438987"
     Site            order(288,292)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438987"
     Site            order(298,300,347,360..361,366..367)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438987"
     Site            order(302,305,321)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438987"
     Site            303..306
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438987"
     Region          581..616
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..620
                     /gene="LOC117609809"
                     /coded_by="XM_034336516.2:124..1986"
                     /db_xref="GeneID:117609809"
ORIGIN      
        1 msirrrtdpf mtqriwdaik itvhqrslps ndrmvrhlsr vygiteqeaq eelnkavddg
       61 lvylkrvptk sgieqesyrl padtvsnddh dwycckcqka gvvecceqch rvyhpechvp
      121 nniklkmchf cesinndtyq dkidlnhils ftcghlkakl ppeitnrtiv fnhdpivtan
      181 nkysgptwis egedawrpnv likhhmdlai mdskiknney nnlaefrada hnilhniiiy
      241 hgahsivgem gltmyqdccy dlqeirrcad cyrisnekse kmwfcipcnp phqlvyakqk
      301 gypywpakvm qvngniydvr ffgghhmran iekvfirpit sslqslqpse kgkwkemkik
      361 rstawnrafe elkhhqnlll klgkyindld inndsdgpkp tkirnieshp knstkqllkd
      421 lrvkverlss dshndilptq eatdnnerst ikakseerqv pclpvaedep argisstcpq
      481 glkkegsqed mvtsssqepr tkcvlvqteq iqtetlpaki krerrtseqp tttaveklrr
      541 elelekckel eklqaehake lrqltdrhqq iiseikkkqw cynceaeaiy hccwntaycs
      601 tdcqqvhwqr ehkrvcrrkr
//
LOCUS       XP_034192417             609 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger MYND domain-containing protein 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192417
VERSION     XP_034192417.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336526.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..609
                     /product="zinc finger MYND domain-containing protein 11
                     isoform X2"
                     /calculated_mol_wt=70828
     Region          93..>121
                     /region_name="PHD_SF"
                     /note="PHD finger superfamily; cl22851"
                     /db_xref="CDD:473978"
     Site            order(93,103..106,110)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          145..268
                     /region_name="Bromo_ZMYND11"
                     /note="Bromodomain; ZMYND11_like sub-family. ZMYND11 or
                     BS69 is a ubiquitously expressed nuclear protein that has
                     been shown to associate with chromatin. It interacts with
                     chromatin remodeling factors and might play a role in
                     chromatin remodeling and gene...; cd05492"
                     /db_xref="CDD:99924"
     Site            order(175,195,198,237,241,247)
                     /site_type="other"
                     /note="putative acetyllysine binding site [chemical
                     binding]"
                     /db_xref="CDD:99924"
     Region          286..370
                     /region_name="PWWP_BS69"
                     /note="PWWP domain found in protein BS69 and similar
                     proteins; cd20159"
                     /db_xref="CDD:438987"
     Site            order(288,292)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:438987"
     Site            order(298,300,347,349..350,355..356)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438987"
     Site            order(302,305,321)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438987"
     Site            303..306
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438987"
     Region          570..605
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..609
                     /gene="LOC117609809"
                     /coded_by="XM_034336526.2:237..2066"
                     /db_xref="GeneID:117609809"
ORIGIN      
        1 msirrrtdpf mtqriwdaik itvhqrslps ndrmvrhlsr vygiteqeaq eelnkavddg
       61 lvylkrvptk sgieqesyrl padtvsnddh dwycckcqka gvvecceqch rvyhpechvp
      121 nniklkmchf cesinndtyq dkidlnhils ftcghlkakl ppeitnrtiv fnhdpivtan
      181 nkysgptwis egedawrpnv likhhmdlai mdskiknney nnlaefrada hnilhniiiy
      241 hgahsivgem gltmyqdccy dlqeirrcad cyrisnekse kmwfcipcnp phqlvyakqk
      301 gypywpakvm qvngniydvr ffgghhmran iekvfirpit sslqslqikr stawnrafee
      361 lkhhqnlllk lgkyindldi nndsdgpkpt kirnieshpk nstkqllkdl rvkverlssd
      421 shndilptqe atdnnersti kakseerqvp clpvaedepa rgisstcpqg lkkegsqedm
      481 vtsssqeprt kcvlvqteqi qtetlpakik rerrtseqpt ttaveklrre lelekckele
      541 klqaehakel rqltdrhqqi iseikkkqwc ynceaeaiyh ccwntaycst dcqqvhwqre
      601 hkrvcrrkr
//
LOCUS       XP_034192432             248 aa            linear   INV 03-SEP-2025
DEFINITION  putative phosphatase phospho2 [Osmia lignaria lignaria].
ACCESSION   XP_034192432
VERSION     XP_034192432.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336541.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192432.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="putative phosphatase phospho2"
                     /calculated_mol_wt=28188
     Region          5..241
                     /region_name="HAD_like"
                     /note="Haloacid Dehalogenase-like Hydrolases; cl21460"
                     /db_xref="CDD:473868"
     CDS             1..248
                     /gene="LOC117609818"
                     /coded_by="XM_034336541.2:237..983"
                     /db_xref="GeneID:117609818"
ORIGIN      
        1 mhrsvlvafd fdhtiandnt dvvarkllpk eklpdsvkdl yrssgwipym gkifellhsn
       61 sidvkqikna ivnippvpgi dkllrelhsr gceiiiisds ntlfisewlq sknlnhvite
      121 tftnpakigd dgmirvdmyh iqnscklstv nlckgeiles yikkrndegi hfdrvvyvgd
      181 gkndlcpilr lserdlafpr kdymlvkiln dsknyevpki qaavfpwhdg iqilnkleee
      241 iglsqssm
//
LOCUS       XP_034192450             192 aa            linear   INV 03-SEP-2025
DEFINITION  protein LZIC [Osmia lignaria lignaria].
ACCESSION   XP_034192450
VERSION     XP_034192450.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336559.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..192
                     /product="protein LZIC"
                     /calculated_mol_wt=21657
     Region          <8..152
                     /region_name="Nup88"
                     /note="Nuclear pore component; pfam10168"
                     /db_xref="CDD:462975"
     Region          113..190
                     /region_name="ICAT"
                     /note="Beta-catenin-interacting protein ICAT; pfam06384"
                     /db_xref="CDD:399405"
     CDS             1..192
                     /gene="LOC117609835"
                     /coded_by="XM_034336559.2:223..801"
                     /db_xref="GeneID:117609835"
ORIGIN      
        1 mssrgklete klrrnleaql drlvqqledi eenrnlldad ayeeamqltk edlqsfnesl
       61 qrmisgdttl vdelssiqla tqsaiseafk tpavirmfgk retaqlrtrl aqldcdmklg
      121 klsteasdrq rgeilnalrq lgeklepqel qlleklrlns idttnyvqit estekgqmai
      181 avvgdevrtq nt
//
LOCUS       XP_034192518             569 aa            linear   INV 03-SEP-2025
DEFINITION  chronologically inappropriate morphogenesis [Osmia lignaria
            lignaria].
ACCESSION   XP_034192518
VERSION     XP_034192518.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336627.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..569
                     /product="chronologically inappropriate morphogenesis"
                     /calculated_mol_wt=60333
     Region          93..178
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          498..519
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          526..543
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..569
                     /gene="chinmo"
                     /coded_by="XM_034336627.2:66..1775"
                     /db_xref="GeneID:117609873"
ORIGIN      
        1 myapvgeghg nqqpslvnpw wlhppagrtv gherdqegky lheggrdrgg eghgvgpkgg
       61 imdshqhqfc lkwnsfgsnl atafsnlfks esltdvtlfc egvtfkahrl ilaacskhfq
      121 elfegmppsp aglivildgt sahnmaslle fmyrgevhvs qeslssflka aeclqvkgls
      181 ieheklavaq rhaaennnss tdagggnggg gdgggggggg ggggggsgge sgsgvsgsvs
      241 gnvagsvggs vgggigsvgg gvgevsdlge ageatmvrni tkrtptpaps papaytvpsl
      301 ystshyyesp qkrlmrspsd vdtlgrasvl rdgtafrpas aphplllenh fyqpfihpse
      361 tsahphtaph tpteleqele rarseersnc dlkesvaedl rikqepvals dreraeklae
      421 rlsgevysgg rgfdgshygs nsdqmqhggq aghgmmplpp ahpptpdlsp apttpamwnt
      481 kinkagtvtt pdgkklkcpf cerlygyetn lrahirqrhq girvpcpfcs rtftrnntvr
      541 rhiarehkte lnmkafqqsn hsmsdsvpq
//
LOCUS       XP_034192545             557 aa            linear   INV 03-SEP-2025
DEFINITION  neither inactivation nor afterpotential protein G [Osmia lignaria
            lignaria].
ACCESSION   XP_034192545
VERSION     XP_034192545.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336654.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192545.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="neither inactivation nor afterpotential protein
                     G"
                     /calculated_mol_wt=62390
     Region          32..555
                     /region_name="BetA"
                     /note="Choline dehydrogenase or related flavoprotein
                     [Lipid transport and metabolism, General function
                     prediction only]; COG2303"
                     /db_xref="CDD:441878"
     CDS             1..557
                     /gene="LOC117609893"
                     /coded_by="XM_034336654.2:83..1756"
                     /db_xref="GeneID:117609893"
ORIGIN      
        1 mwnyvlfssl vlivsllyhc yfnsplsiie hpnthydyii vgagtagcvv alrlsealnv
       61 tvllveaggy fgwvssvpil apvmqgtevd wsfstepqmf ssrgfwnhiq kvpqgkglgg
      121 sgqmnhlvhs fgrsedykaw pkgwshadll pyfkkvsdim nvmsspekey laeaflvaee
      181 slklnnvtlq rglyttkrgs rwstfnahlq nawnrinlhi ltntlvskil fkenlnvdgi
      241 kviykdglmr kivarkevil cagtintpql lllsgigpae dldkfqipvv snvsevgknl
      301 fdhfmlpvyv nleakvsitl vklqtlpevl nyfifgrgwy atngimavgr vnnsglmlfg
      361 lgttdermlk plsnyrtepy rslypsyknn sqegflflty clqpksrgtv tlrstkirhq
      421 pkidpayleh yddilcthea infairviet kqfreygakv hhpdleecrh lpqdyrdiey
      481 secvmrvgal sshhvggtck mgvddhsvvd enlrvrgvng lriidasvip spvsgnpnsv
      541 liamaerasd liigrtg
//
LOCUS       XP_034196280             450 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611917 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196280
VERSION     XP_034196280.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340389.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="uncharacterized protein LOC117611917 isoform X1"
                     /calculated_mol_wt=48057
     Region          107..181
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(116,118,120,124,127,131..132)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          212..282
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     Region          <214..>403
                     /region_name="hnRNP-R-Q"
                     /note="heterogeneous nuclear ribonucleoprotein R, Q
                     family; TIGR01648"
                     /db_xref="CDD:273732"
     CDS             1..450
                     /gene="LOC117611917"
                     /coded_by="XM_034340389.2:2874..4226"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mlfvpftvpt fpathhqtth pahqthhqql qattqnpvqq pqqltqlnql shlnhqgivp
       61 attnqptihs tscspqqqat tpnkemkvnw vtspgnqpkq dtskhyhifv gdlspeietq
      121 tlreafapfg eisdcrvvrd pqtlkskgyg fvsfvkkaea esaigamhgq wlgsrsirtn
      181 watrkppapk seanakpltf devynqsspt nctvycgglt nglteelmqk tfspfgsiqe
      241 irvfkdkgya firfstkesa thaivavhnt dingqtvkcs wgkesgdpnn aqqtgqalss
      301 atypyyaaaa aaaaaaagva gvggvggvgg agqlgywypp qsypttatqm qaggflqgmq
      361 gytygqfagy qqaqymgmgm gvhaagtaag wgqglpggpq lpphhattvt appgvqaapp
      421 pppppaqmma ypmqqfqlad edwltpsllv
//
LOCUS       XP_034196282             447 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611917 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196282
VERSION     XP_034196282.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340391.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..447
                     /product="uncharacterized protein LOC117611917 isoform X2"
                     /calculated_mol_wt=47448
     Region          107..181
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(116,118,120,124,127,131..132)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          212..282
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     Region          <214..>403
                     /region_name="hnRNP-R-Q"
                     /note="heterogeneous nuclear ribonucleoprotein R, Q
                     family; TIGR01648"
                     /db_xref="CDD:273732"
     CDS             1..447
                     /gene="LOC117611917"
                     /coded_by="XM_034340391.2:2876..4219"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mlfvpftvpt fpathhqtth pahqthhqql qattqnpvqq pqqltqlnql shlnhqgivp
       61 attnqptihs tscspqqqat tpnkemkvnw vtspgnqpkq dtskhyhifv gdlspeietq
      121 tlreafapfg eisdcrvvrd pqtlkskgyg fvsfvkkaea esaigamhgq wlgsrsirtn
      181 watrkppapk seanakpltf devynqsspt nctvycgglt nglteelmqk tfspfgsiqe
      241 irvfkdkgya firfstkesa thaivavhnt dingqtvkcs wgkesgdpnn aqqtgqalss
      301 atypyyaaaa aaaaaaagva gvggvggvgg agqlgywypp qsypttatqm qaggflqgmq
      361 gytygqfagy qqaqymgmgm gvhaagtaag wgqglpggpq lpphhattvt appgvqaapp
      421 pppppaqmma ypmqqfqcvl gcgpasg
//
LOCUS       XP_034196283             439 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611917 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196283
VERSION     XP_034196283.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340392.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..439
                     /product="uncharacterized protein LOC117611917 isoform X3"
                     /calculated_mol_wt=46803
     Region          107..181
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(116,118,120,124,127,131..132)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          212..282
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     Region          <214..>403
                     /region_name="hnRNP-R-Q"
                     /note="heterogeneous nuclear ribonucleoprotein R, Q
                     family; TIGR01648"
                     /db_xref="CDD:273732"
     CDS             1..439
                     /gene="LOC117611917"
                     /coded_by="XM_034340392.2:2875..4194"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mlfvpftvpt fpathhqtth pahqthhqql qattqnpvqq pqqltqlnql shlnhqgivp
       61 attnqptihs tscspqqqat tpnkemkvnw vtspgnqpkq dtskhyhifv gdlspeietq
      121 tlreafapfg eisdcrvvrd pqtlkskgyg fvsfvkkaea esaigamhgq wlgsrsirtn
      181 watrkppapk seanakpltf devynqsspt nctvycgglt nglteelmqk tfspfgsiqe
      241 irvfkdkgya firfstkesa thaivavhnt dingqtvkcs wgkesgdpnn aqqtgqalss
      301 atypyyaaaa aaaaaaagva gvggvggvgg agqlgywypp qsypttatqm qaggflqgmq
      361 gytygqfagy qqaqymgmgm gvhaagtaag wgqglpggpq lpphhattvt appgvqaapp
      421 pppppaqmma ypmqqfqaq
//
LOCUS       XP_034196284             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196284
VERSION     XP_034196284.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340393.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340393.2:843..2063"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196288             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196288
VERSION     XP_034196288.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340397.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340397.2:644..1864"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196285             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196285
VERSION     XP_034196285.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340394.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340394.2:816..2036"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196287             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196287
VERSION     XP_034196287.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340396.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340396.2:734..1954"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196289             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196289
VERSION     XP_034196289.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340398.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340398.2:1478..2698"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196291             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196291
VERSION     XP_034196291.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340400.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340400.2:617..1837"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196296             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196296
VERSION     XP_034196296.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340405.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340405.2:535..1755"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196292             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196292
VERSION     XP_034196292.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340401.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340401.2:1498..2718"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196295             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196295
VERSION     XP_034196295.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340404.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340404.2:605..1825"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196297             406 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196297
VERSION     XP_034196297.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340406.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="nucleolysin TIAR isoform X4"
                     /calculated_mol_wt=42895
     Region          63..137
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Region          71..>347
                     /region_name="ELAV_HUD_SF"
                     /note="ELAV/HuD family splicing factor; TIGR01661"
                     /db_xref="CDD:273741"
     Site            order(72,74,76,80,83,87..88)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          168..238
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     CDS             1..406
                     /gene="LOC117611917"
                     /coded_by="XM_034340406.2:1342..2562"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mavvpgknkl kmgsagryys ayliydvans claalhakmp ppvvvtrtnp pspvhvqnpq
       61 hyhifvgdls peietqtlre afapfgeisd crvvrdpqtl kskgygfvsf vkkaeaesai
      121 gamhgqwlgs rsirtnwatr kppapksean akpltfdevy nqssptnctv ycggltnglt
      181 eelmqktfsp fgsiqeirvf kdkgyafirf stkesathai vavhntding qtvkcswgke
      241 sgdpnnaqqt gqalssatyp yyaaaaaaaa aaagvagvgg vggvggagql gywyppqsyp
      301 ttatqmqagg flqgmqgyty gqfagyqqaq ymgmgmgvha agtaagwgqg lpggpqlpph
      361 hattvtappg vqaapppppp paqmmaypmq qfqladedwl tpsllv
//
LOCUS       XP_034196298             399 aa            linear   INV 03-SEP-2025
DEFINITION  cytotoxic granule associated RNA binding protein TIA1 isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034196298
VERSION     XP_034196298.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340407.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="cytotoxic granule associated RNA binding protein
                     TIA1 isoform X5"
                     /calculated_mol_wt=42013
     Region          56..130
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(65,67,69,73,76,80..81)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          161..231
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     Region          <163..>352
                     /region_name="hnRNP-R-Q"
                     /note="heterogeneous nuclear ribonucleoprotein R, Q
                     family; TIGR01648"
                     /db_xref="CDD:273732"
     CDS             1..399
                     /gene="LOC117611917"
                     /coded_by="XM_034340407.2:1053..2252"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mltmstlmmp ttmttmtnpg pipahsmpqk ieppkltaaa tlthtaaaan ksehyhifvg
       61 dlspeietqt lreafapfge isdcrvvrdp qtlkskgygf vsfvkkaeae saigamhgqw
      121 lgsrsirtnw atrkppapks eanakpltfd evynqssptn ctvycggltn glteelmqkt
      181 fspfgsiqei rvfkdkgyaf irfstkesat haivavhntd ingqtvkcsw gkesgdpnna
      241 qqtgqalssa typyyaaaaa aaaaaagvag vggvggvgga gqlgywyppq sypttatqmq
      301 aggflqgmqg ytygqfagyq qaqymgmgmg vhaagtaagw gqglpggpql pphhattvta
      361 ppgvqaappp ppppaqmmay pmqqfqlade dwltpsllv
//
LOCUS       XP_034196299             365 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolysin TIAR isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034196299
VERSION     XP_034196299.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340408.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="nucleolysin TIAR isoform X6"
                     /calculated_mol_wt=38590
     Region          22..96
                     /region_name="RRM2_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins p40-TIA-1 and
                     TIAR; cd12353"
                     /db_xref="CDD:409789"
     Site            order(31,33,35,39,42,46..47)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:409789"
     Region          127..197
                     /region_name="RRM3_TIA1_like"
                     /note="RNA recognition motif 2 (RRM2) found in
                     granule-associated RNA binding proteins (p40-TIA-1 and
                     TIAR), and yeast nuclear and cytoplasmic polyadenylated
                     RNA-binding protein PUB1; cd12354"
                     /db_xref="CDD:409790"
     Region          <129..>318
                     /region_name="hnRNP-R-Q"
                     /note="heterogeneous nuclear ribonucleoprotein R, Q
                     family; TIGR01648"
                     /db_xref="CDD:273732"
     CDS             1..365
                     /gene="LOC117611917"
                     /coded_by="XM_034340408.2:231..1328"
                     /db_xref="GeneID:117611917"
ORIGIN      
        1 mkvnwvtspg nqpkqdtskh yhifvgdlsp eietqtlrea fapfgeisdc rvvrdpqtlk
       61 skgygfvsfv kkaeaesaig amhgqwlgsr sirtnwatrk ppapkseana kpltfdevyn
      121 qssptnctvy cggltnglte elmqktfspf gsiqeirvfk dkgyafirfs tkesathaiv
      181 avhntdingq tvkcswgkes gdpnnaqqtg qalssatypy yaaaaaaaaa aagvagvggv
      241 ggvggagqlg ywyppqsypt tatqmqaggf lqgmqgytyg qfagyqqaqy mgmgmgvhaa
      301 gtaagwgqgl pggpqlpphh attvtappgv qaappppppp aqmmaypmqq fqladedwlt
      361 psllv
//
LOCUS       XP_034193111             339 aa            linear   INV 03-SEP-2025
DEFINITION  transforming growth factor beta regulator 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193111
VERSION     XP_034193111.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337220.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="transforming growth factor beta regulator 1"
                     /calculated_mol_wt=38427
     Region          151..201
                     /region_name="FYRN"
                     /note="F/Y-rich N-terminus; pfam05964"
                     /db_xref="CDD:461787"
     Region          207..277
                     /region_name="FYRC"
                     /note="F/Y rich C-terminus; cl02651"
                     /db_xref="CDD:470645"
     CDS             1..339
                     /gene="LOC117610165"
                     /coded_by="XM_034337220.2:70..1089"
                     /db_xref="GeneID:117610165"
ORIGIN      
        1 mtqaydsyyn dfqknaelqq nlkykkkyrr lkriikdtvf enaalcdqva qmqenlmlvk
       61 eerlfllrkl cqqqgdidps smvarsqsnn insgsfntec stpkktakkr vstdgsetkh
      121 kakrynktir kvvqlipldv hgrpifpial gdltvyslge vvsdriayht edliypvgyc
      181 strvyanlrd prtkslytck ildggqkprf eivsdndldq slvgstpdec hskllmtisp
      241 alrsitpkga dffgishptv qnliqstpgt rklamykqqr fevsknqsve rgtspiteee
      301 tdpglgfaal hrhyaltsty rikeepddkd lltlqefla
//
LOCUS       XP_076546516             326 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611630 [Osmia lignaria lignaria].
ACCESSION   XP_076546516
VERSION     XP_076546516.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690401.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="uncharacterized protein LOC117611630"
                     /calculated_mol_wt=36119
     Region          217..299
                     /region_name="RRM_RBM42"
                     /note="RNA recognition motif (RRM) found in RNA-binding
                     protein 42 (RBM42) and similar proteins; cd12383"
                     /db_xref="CDD:409817"
     CDS             1..326
                     /gene="LOC117611630"
                     /coded_by="XM_076690401.1:162..1142"
                     /db_xref="GeneID:117611630"
ORIGIN      
        1 meeklrqmed emnrfeaeig sqlvtpmvrp vigantynqv arqleqhelp tpvvaaaaas
       61 lafppmmgfp pppppppppp ppppmlipqq varqgtvpsi ttysspaqis npylsnmvdp
      121 cltatpktye ststpmihpe iieqiintki peddskkkpk vkgvstaael aisqgkassi
      181 manasaeesh pkgkgkknkk ivrmaggqiw edpsllewde ddfrifcgdl gndvtdemlv
      241 rvfgkypsfq rakvvrdkrt nktkgfgfvs fkdpqdfika mkemngryvg srpiklrkss
      301 wkqrnletvr kkekekqali glltgr
//
LOCUS       XP_034193124             318 aa            linear   INV 03-SEP-2025
DEFINITION  EI24 domain-containing protein tank isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193124
VERSION     XP_034193124.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337233.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="EI24 domain-containing protein tank isoform X1"
                     /calculated_mol_wt=35725
     Region          122..259
                     /region_name="EI24"
                     /note="Etoposide-induced protein 2.4 (EI24); pfam07264"
                     /db_xref="CDD:462129"
     CDS             1..318
                     /gene="tank"
                     /coded_by="XM_034337233.2:305..1261"
                     /db_xref="GeneID:117610171"
ORIGIN      
        1 mdnvtgiiha iyrgfvdslr gaivlfymdk rinekllkqs psriethgkd mavahnpskc
       61 ynqlreskvl krtiqccaln ggvfwasili fecgilpllk yllsiifghs ptmgmtvwsw
      121 tkpflsltfg tvwvlplfll srivnslwfq diadsayryr qgrplllssv sklvadtlfs
      181 ilvqalflgq gmlvsriplp pvgdilalih mcllyalyaf eykwfnmgwe lhrrlsfies
      241 nwpyfvgfgl plavltqmpn syvisgcvfs ilfplfivsg neaepvtgvc dcplklfspv
      301 iaiantlfnk tigpanrr
//
LOCUS       XP_034193143             318 aa            linear   INV 03-SEP-2025
DEFINITION  EI24 domain-containing protein tank isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193143
VERSION     XP_034193143.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337252.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="EI24 domain-containing protein tank isoform X1"
                     /calculated_mol_wt=35725
     Region          122..259
                     /region_name="EI24"
                     /note="Etoposide-induced protein 2.4 (EI24); pfam07264"
                     /db_xref="CDD:462129"
     CDS             1..318
                     /gene="tank"
                     /coded_by="XM_034337252.2:342..1298"
                     /db_xref="GeneID:117610171"
ORIGIN      
        1 mdnvtgiiha iyrgfvdslr gaivlfymdk rinekllkqs psriethgkd mavahnpskc
       61 ynqlreskvl krtiqccaln ggvfwasili fecgilpllk yllsiifghs ptmgmtvwsw
      121 tkpflsltfg tvwvlplfll srivnslwfq diadsayryr qgrplllssv sklvadtlfs
      181 ilvqalflgq gmlvsriplp pvgdilalih mcllyalyaf eykwfnmgwe lhrrlsfies
      241 nwpyfvgfgl plavltqmpn syvisgcvfs ilfplfivsg neaepvtgvc dcplklfspv
      301 iaiantlfnk tigpanrr
//
LOCUS       XP_034193156             318 aa            linear   INV 03-SEP-2025
DEFINITION  EI24 domain-containing protein tank isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193156
VERSION     XP_034193156.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337265.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="EI24 domain-containing protein tank isoform X1"
                     /calculated_mol_wt=35725
     Region          122..259
                     /region_name="EI24"
                     /note="Etoposide-induced protein 2.4 (EI24); pfam07264"
                     /db_xref="CDD:462129"
     CDS             1..318
                     /gene="tank"
                     /coded_by="XM_034337265.2:176..1132"
                     /db_xref="GeneID:117610171"
ORIGIN      
        1 mdnvtgiiha iyrgfvdslr gaivlfymdk rinekllkqs psriethgkd mavahnpskc
       61 ynqlreskvl krtiqccaln ggvfwasili fecgilpllk yllsiifghs ptmgmtvwsw
      121 tkpflsltfg tvwvlplfll srivnslwfq diadsayryr qgrplllssv sklvadtlfs
      181 ilvqalflgq gmlvsriplp pvgdilalih mcllyalyaf eykwfnmgwe lhrrlsfies
      241 nwpyfvgfgl plavltqmpn syvisgcvfs ilfplfivsg neaepvtgvc dcplklfspv
      301 iaiantlfnk tigpanrr
//
LOCUS       XP_034193150             318 aa            linear   INV 03-SEP-2025
DEFINITION  EI24 domain-containing protein tank isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193150
VERSION     XP_034193150.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337259.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="EI24 domain-containing protein tank isoform X1"
                     /calculated_mol_wt=35725
     Region          122..259
                     /region_name="EI24"
                     /note="Etoposide-induced protein 2.4 (EI24); pfam07264"
                     /db_xref="CDD:462129"
     CDS             1..318
                     /gene="tank"
                     /coded_by="XM_034337259.2:81..1037"
                     /db_xref="GeneID:117610171"
ORIGIN      
        1 mdnvtgiiha iyrgfvdslr gaivlfymdk rinekllkqs psriethgkd mavahnpskc
       61 ynqlreskvl krtiqccaln ggvfwasili fecgilpllk yllsiifghs ptmgmtvwsw
      121 tkpflsltfg tvwvlplfll srivnslwfq diadsayryr qgrplllssv sklvadtlfs
      181 ilvqalflgq gmlvsriplp pvgdilalih mcllyalyaf eykwfnmgwe lhrrlsfies
      241 nwpyfvgfgl plavltqmpn syvisgcvfs ilfplfivsg neaepvtgvc dcplklfspv
      301 iaiantlfnk tigpanrr
//
LOCUS       XP_076546517             291 aa            linear   INV 03-SEP-2025
DEFINITION  EI24 domain-containing protein tank isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546517
VERSION     XP_076546517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690402.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="EI24 domain-containing protein tank isoform X2"
                     /calculated_mol_wt=32701
     Region          95..232
                     /region_name="EI24"
                     /note="Etoposide-induced protein 2.4 (EI24); pfam07264"
                     /db_xref="CDD:462129"
     CDS             1..291
                     /gene="tank"
                     /coded_by="XM_076690402.1:176..1051"
                     /db_xref="GeneID:117610171"
ORIGIN      
        1 mdkrinekll kqspsrieth gkdmavahnp skcynqlres kvlkrtiqcc alnggvfwas
       61 ilifecgilp llkyllsiif ghsptmgmtv wswtkpflsl tfgtvwvlpl fllsrivnsl
      121 wfqdiadsay ryrqgrplll ssvsklvadt lfsilvqalf lgqgmlvsri plppvgdila
      181 lihmcllyal yafeykwfnm gwelhrrlsf iesnwpyfvg fglplavltq mpnsyvisgc
      241 vfsilfplfi vsgneaepvt gvcdcplklf spviaiantl fnktigpanr r
//
LOCUS       XP_076546518             307 aa            linear   INV 03-SEP-2025
DEFINITION  radial spoke head 1 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546518
VERSION     XP_076546518.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690403.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="radial spoke head 1 homolog isoform X1"
                     /calculated_mol_wt=35594
     Region          <3..166
                     /region_name="COG4642"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:443680"
     CDS             1..307
                     /gene="LOC117610223"
                     /coded_by="XM_076690403.1:65..988"
                     /db_xref="GeneID:117610223"
ORIGIN      
        1 mseypeitge pgeeetnplg lyegernekg drhgrgkall pngdmyegqy ckglrhgpgl
       61 yvfkngaryd gqyrqgvkyg qgtfwypdgt ryegewkrdt kygfgvyyyv ngdvyegswk
      121 kdlrhgmgsy lyadtntkfm gtwimdrmqg pgqlihprhr fhgywelnlp ygrgcftfen
      181 acmqhghyih vkdpdyvets eenkdetqpq penvektekk estgpppfep pplkkgfipl
      241 wrarcitpyn pdllppepvp lqeevsldsl vdkcsedisr aeeylkyede yegeyeeyyg
      301 endinni
//
LOCUS       XP_076546519             284 aa            linear   INV 03-SEP-2025
DEFINITION  radial spoke head 1 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546519
VERSION     XP_076546519.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690404.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="radial spoke head 1 homolog isoform X2"
                     /calculated_mol_wt=32803
     Region          <3..157
                     /region_name="COG4642"
                     /note="Uncharacterized conserved protein [Function
                     unknown]"
                     /db_xref="CDD:443680"
     CDS             1..284
                     /gene="LOC117610223"
                     /coded_by="XM_076690404.1:65..919"
                     /db_xref="GeneID:117610223"
ORIGIN      
        1 mseypeitge pgeeetnplg lyegernekg drhgrgkall pngdmyegqy ckglrhgpgl
       61 yvfkngaryd gqyrqgvkyg qgtfwypdgt ryegswkkdl rhgmgsylya dtntkfmgtw
      121 imdrmqgpgq lihprhrfhg ywelnlpygr gcftfenacm qhghyihvkd pdyvetseen
      181 kdetqpqpen vektekkest gpppfepppl kkgfiplwra rcitpynpdl lppepvplqe
      241 evsldslvdk csedisraee ylkyedeyeg eyeeyygend inni
//
LOCUS       XP_034193169             280 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610194 [Osmia lignaria lignaria].
ACCESSION   XP_034193169
VERSION     XP_034193169.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337278.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="uncharacterized protein LOC117610194"
                     /calculated_mol_wt=31170
     CDS             1..280
                     /gene="LOC117610194"
                     /coded_by="XM_034337278.2:253..1095"
                     /db_xref="GeneID:117610194"
ORIGIN      
        1 mhavvsnafn anrmywlqat nireemplsq inqsnfints esvnpgteva tirytappsy
       61 hniilplghp stltadrgap psyeeaidpn apppsydslf grmreahkvs kgvfdflinv
      121 ivlllgtigc tiilgvtmav picmmviggl ylydcpqgey ipvyllvggg fgvfkqllhl
      181 sakvrqrqee rdeerirqsp tqtlincfml gwfiigsmwv ykeyepnydp algkycnktl
      241 ylfafwlits vyiclgvita glcsisvasi vfqrpppdlm
//
LOCUS       XP_034193179             280 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610194 [Osmia lignaria lignaria].
ACCESSION   XP_034193179
VERSION     XP_034193179.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337288.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="uncharacterized protein LOC117610194"
                     /calculated_mol_wt=31170
     CDS             1..280
                     /gene="LOC117610194"
                     /coded_by="XM_034337288.2:184..1026"
                     /db_xref="GeneID:117610194"
ORIGIN      
        1 mhavvsnafn anrmywlqat nireemplsq inqsnfints esvnpgteva tirytappsy
       61 hniilplghp stltadrgap psyeeaidpn apppsydslf grmreahkvs kgvfdflinv
      121 ivlllgtigc tiilgvtmav picmmviggl ylydcpqgey ipvyllvggg fgvfkqllhl
      181 sakvrqrqee rdeerirqsp tqtlincfml gwfiigsmwv ykeyepnydp algkycnktl
      241 ylfafwlits vyiclgvita glcsisvasi vfqrpppdlm
//
LOCUS       XP_034193189             280 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610194 [Osmia lignaria lignaria].
ACCESSION   XP_034193189
VERSION     XP_034193189.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337298.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="uncharacterized protein LOC117610194"
                     /calculated_mol_wt=31170
     CDS             1..280
                     /gene="LOC117610194"
                     /coded_by="XM_034337298.2:135..977"
                     /db_xref="GeneID:117610194"
ORIGIN      
        1 mhavvsnafn anrmywlqat nireemplsq inqsnfints esvnpgteva tirytappsy
       61 hniilplghp stltadrgap psyeeaidpn apppsydslf grmreahkvs kgvfdflinv
      121 ivlllgtigc tiilgvtmav picmmviggl ylydcpqgey ipvyllvggg fgvfkqllhl
      181 sakvrqrqee rdeerirqsp tqtlincfml gwfiigsmwv ykeyepnydp algkycnktl
      241 ylfafwlits vyiclgvita glcsisvasi vfqrpppdlm
//
LOCUS       XP_034193230             207 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610218 [Osmia lignaria lignaria].
ACCESSION   XP_034193230
VERSION     XP_034193230.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193230.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..207
                     /product="uncharacterized protein LOC117610218"
                     /calculated_mol_wt=22701
     CDS             1..207
                     /gene="LOC117610218"
                     /coded_by="XM_034337339.2:248..871"
                     /db_xref="GeneID:117610218"
ORIGIN      
        1 mlfgvtvfvy alnslwvvcq avplldlrnt leqnvldami tsacssngec wewlpvqevh
       61 kniatvpdnl pilktptrrq tsnidpntii eswddnvptl eknslsplaa krngnrlskk
      121 dvlmsrgwga ggmpfsvlym nphaprgnya gneqqhelgk avdsptpamg qsnsriavrn
      181 ggptlprkqy siipqlfisy gwgpfgk
//
LOCUS       XP_076546520             186 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610206 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546520
VERSION     XP_076546520.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690405.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..186
                     /product="uncharacterized protein LOC117610206 isoform X1"
                     /calculated_mol_wt=21345
     CDS             1..186
                     /gene="LOC117610206"
                     /coded_by="XM_076690405.1:101..661"
                     /db_xref="GeneID:117610206"
ORIGIN      
        1 mqetrqkmkf cskngiykpr disgagcgir tnskirlrgg rdhiwmspsl hpsitnvasi
       61 hrksyekfhn plkefiedvg dtkkiqnkhs lyqpakvgir kikqvhvptk fkkvvipknn
      121 tklscpilkn myidstvdss stlimkrran ikvrvcilfe nniiiipstr ehtcfaqlre
      181 vlhsyf
//
LOCUS       XP_034193208             152 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610206 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193208
VERSION     XP_034193208.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337317.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..152
                     /product="uncharacterized protein LOC117610206 isoform X2"
                     /calculated_mol_wt=17300
     CDS             1..152
                     /gene="LOC117610206"
                     /coded_by="XM_034337317.2:96..554"
                     /db_xref="GeneID:117610206"
ORIGIN      
        1 mqetrqkmkf cskngiykpr disgagcgir tnskirlrgg rdhiwmspsl hpsitnvasi
       61 hrksyekfhn plkefiedvg dtkkiqnkhs lyqpakvgir kikqvhvptk fkkvvipknn
      121 tklscpilkn myidstvdss stlimkrran ik
//
LOCUS       XP_076546521             163 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305717 [Osmia lignaria lignaria].
ACCESSION   XP_076546521
VERSION     XP_076546521.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690406.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..163
                     /product="uncharacterized protein LOC143305717"
                     /calculated_mol_wt=17881
     CDS             1..163
                     /gene="LOC143305717"
                     /coded_by="XM_076690406.1:92..583"
                     /db_xref="GeneID:143305717"
ORIGIN      
        1 mqssipklll vsvlcvipyv maenkvtvld fkadvpgndv idswshdltn nvissninik
       61 kncpgaieae ikvykgddlv nqmvqnlkqp ikdfesykic esidspdled dscsiaegeq
      121 sakncdisgw fsdmspgdyk vecvfkqadv ittltlnvki esd
//
LOCUS       XP_034192461             991 aa            linear   INV 03-SEP-2025
DEFINITION  zona pellucida domain protein papillote [Osmia lignaria lignaria].
ACCESSION   XP_034192461
VERSION     XP_034192461.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336570.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..991
                     /product="zona pellucida domain protein papillote"
                     /calculated_mol_wt=110386
     Region          66..>150
                     /region_name="ZP"
                     /note="Zona pellucida (ZP) domain; smart00241"
                     /db_xref="CDD:214579"
     CDS             1..991
                     /gene="pot"
                     /coded_by="XM_034336570.2:267..3242"
                     /db_xref="GeneID:117609844"
ORIGIN      
        1 mlprliavlc talpvvlsqs nyasqgnsie yqpglppstv lngkvtkldd issmiflnrt
       61 kaylncsqgs mqvelkfeep fygvayadfd rnsacifkgr gstsaklelp lkgcgtrqdp
      121 qrvftnnvvv rfhpglemdg devitivcry pppvapkppa ppasilaipa paplpepplk
      181 gfqilliica ilflsllllg lgcsymclkr rnvrvihrhp fgsdsgseit kpstpnlgsi
      241 imfeglkipr prpfhtstst tgsdthlmnp slpsdypset segdeepslp gssagsydkq
      301 vfvhhethqr qemrtnssly ldtvaaemet ssmtamnasr stlprhimav piepkfdvqm
      361 rvtkvpptsp splpsesdia svalernltt ilekeesslt rslesppart tfsyvpelhv
      421 ppggssstst isrqqtppvy srilrkqaer etttstsvlq eripspspve qpplqhheir
      481 rprsltslnt eltetrslte vtdhthakyr vasilaptpp ppppivptvt sthtavqhre
      541 hrlfreevte plvepqvvap rrpeitthev ddvflktvte kktiedverh rrqvteyrak
      601 pppdpkwdvt irnyptenlp ppppewenfs dvssnsnlti tneptgiqtd apideeadvn
      661 ieptytarae ipcrpppvtr rsvdetdadw fsrlsrvldp ryvsefteee rakwreiitt
      721 dstlrtllte avvkedyeli rkdarytnlf ppakwdviir ilgppsmhag stsssthgkn
      781 hgqrykrsks sewdtrsrrs slptlyeyes dggssahths hrlqmsqstt amgqsrlrrl
      841 gsshrgtgga sevdvrsmse mtvrdfarvd rddltslssf egadslvrsl sqpslarsgs
      901 eftehwgips gmmdlppwaa eegsssvest pravrrndrl evlasfddrr qgsattrssr
      961 yverelnsvr vtegqrtsnw fhddsepemq i
//
LOCUS       XP_076546522            1064 aa            linear   INV 03-SEP-2025
DEFINITION  elongation factor-like GTPase 1 [Osmia lignaria lignaria].
ACCESSION   XP_076546522
VERSION     XP_076546522.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690407.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1064
                     /product="elongation factor-like GTPase 1"
                     /calculated_mol_wt=119190
     Region          7..1047
                     /region_name="PRK13351"
                     /note="elongation factor G-like protein; cl46912"
                     /db_xref="CDD:481252"
     CDS             1..1064
                     /gene="LOC117609599"
                     /coded_by="XM_076690407.1:909..4103"
                     /db_xref="GeneID:117609599"
ORIGIN      
        1 mrlvspekls eiqsnpasir nicilahvdh gkttladslv asngiisskl agklryldsr
       61 pdeqlrgitm ksssitlyyq ycleeyvinl idspghvdfa sevstavrlc dgslivvdvv
      121 egvcpqtrsa lkisyaeglk pilvlnkvdr litemklspl daymhltqvl eqvnavmgel
      181 fasdvmeree kenvkkesme ndsernlsdw qsvlddidds glyfspeqgn vlfssatdgw
      241 gfgvkefaki fskklgfsek vllktlwgdy yvnsktkrim kgaqekakkp lfvqlildni
      301 wtlyetitir kdkekvasmt erldiklttr dlrhtdckaq lqavcsqwlp laracldaic
      361 ekvpaplnlt sekverllsg nfdfstlpee tqrlkeafla cdpsvdapvv vfvskmfpve
      421 kkalpenkpk pltseelaqr revarmkhae rllkeqqaee aaqqdinsdh snaegksenp
      481 spegsvkgee ensettliaf ariysgclkk gstvfvlgpk hdprealerv kagelpaeen
      541 vtlkdlkagr hvtkvtiqrl ylmmgrelep tekitagnvf gigdleehvl ktatlsttia
      601 cpsfseltsl gvpimrvale pkhsndlqal inglkllnqa dacalvhiqe sgeivlstag
      661 evhlercled lrvryakvev nvsepivqfr etvvpppkvd mvneaiekkv eegsfsawta
      721 nrqcyfevda rplpdrvtki leknvnlika lyyyygksss eregetkkdk eigktesmys
      781 mserrqrele sfkselaiaf eeagqkdvld riwsfgprnc gsnillnetd ykqqkfwegh
      841 pksidprapy esglmngfql atlagplcee pmmgvcfvvk kweihqdsds ensgqnqsqg
      901 ggylmstfke acrrvfnsrh prlvtpmytc svlvnsdvlg klyaafgkrh grviaadsal
      961 gfggqfrvla tlpvpesfql arelrtqtsg laspqlvfsh wevikqdpyw tpstdeeylh
     1021 fgdkadsenr akkyidavrr rkglpvdsql vthaekqrtl sknk
//
LOCUS       XP_034192054            1064 aa            linear   INV 03-SEP-2025
DEFINITION  elongation factor-like GTPase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034192054
VERSION     XP_034192054.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336163.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192054.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1064
                     /product="elongation factor-like GTPase 1"
                     /calculated_mol_wt=119190
     Region          7..1047
                     /region_name="PRK13351"
                     /note="elongation factor G-like protein; cl46912"
                     /db_xref="CDD:481252"
     CDS             1..1064
                     /gene="LOC117609599"
                     /coded_by="XM_034336163.2:677..3871"
                     /db_xref="GeneID:117609599"
ORIGIN      
        1 mrlvspekls eiqsnpasir nicilahvdh gkttladslv asngiisskl agklryldsr
       61 pdeqlrgitm ksssitlyyq ycleeyvinl idspghvdfa sevstavrlc dgslivvdvv
      121 egvcpqtrsa lkisyaeglk pilvlnkvdr litemklspl daymhltqvl eqvnavmgel
      181 fasdvmeree kenvkkesme ndsernlsdw qsvlddidds glyfspeqgn vlfssatdgw
      241 gfgvkefaki fskklgfsek vllktlwgdy yvnsktkrim kgaqekakkp lfvqlildni
      301 wtlyetitir kdkekvasmt erldiklttr dlrhtdckaq lqavcsqwlp laracldaic
      361 ekvpaplnlt sekverllsg nfdfstlpee tqrlkeafla cdpsvdapvv vfvskmfpve
      421 kkalpenkpk pltseelaqr revarmkhae rllkeqqaee aaqqdinsdh snaegksenp
      481 spegsvkgee ensettliaf ariysgclkk gstvfvlgpk hdprealerv kagelpaeen
      541 vtlkdlkagr hvtkvtiqrl ylmmgrelep tekitagnvf gigdleehvl ktatlsttia
      601 cpsfseltsl gvpimrvale pkhsndlqal inglkllnqa dacalvhiqe sgeivlstag
      661 evhlercled lrvryakvev nvsepivqfr etvvpppkvd mvneaiekkv eegsfsawta
      721 nrqcyfevda rplpdrvtki leknvnlika lyyyygksss eregetkkdk eigktesmys
      781 mserrqrele sfkselaiaf eeagqkdvld riwsfgprnc gsnillnetd ykqqkfwegh
      841 pksidprapy esglmngfql atlagplcee pmmgvcfvvk kweihqdsds ensgqnqsqg
      901 ggylmstfke acrrvfnsrh prlvtpmytc svlvnsdvlg klyaafgkrh grviaadsal
      961 gfggqfrvla tlpvpesfql arelrtqtsg laspqlvfsh wevikqdpyw tpstdeeylh
     1021 fgdkadsenr akkyidavrr rkglpvdsql vthaekqrtl sknk
//
LOCUS       XP_034192064            1064 aa            linear   INV 03-SEP-2025
DEFINITION  elongation factor-like GTPase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034192064
VERSION     XP_034192064.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336173.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192064.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1064
                     /product="elongation factor-like GTPase 1"
                     /calculated_mol_wt=119190
     Region          7..1047
                     /region_name="PRK13351"
                     /note="elongation factor G-like protein; cl46912"
                     /db_xref="CDD:481252"
     CDS             1..1064
                     /gene="LOC117609599"
                     /coded_by="XM_034336173.2:729..3923"
                     /db_xref="GeneID:117609599"
ORIGIN      
        1 mrlvspekls eiqsnpasir nicilahvdh gkttladslv asngiisskl agklryldsr
       61 pdeqlrgitm ksssitlyyq ycleeyvinl idspghvdfa sevstavrlc dgslivvdvv
      121 egvcpqtrsa lkisyaeglk pilvlnkvdr litemklspl daymhltqvl eqvnavmgel
      181 fasdvmeree kenvkkesme ndsernlsdw qsvlddidds glyfspeqgn vlfssatdgw
      241 gfgvkefaki fskklgfsek vllktlwgdy yvnsktkrim kgaqekakkp lfvqlildni
      301 wtlyetitir kdkekvasmt erldiklttr dlrhtdckaq lqavcsqwlp laracldaic
      361 ekvpaplnlt sekverllsg nfdfstlpee tqrlkeafla cdpsvdapvv vfvskmfpve
      421 kkalpenkpk pltseelaqr revarmkhae rllkeqqaee aaqqdinsdh snaegksenp
      481 spegsvkgee ensettliaf ariysgclkk gstvfvlgpk hdprealerv kagelpaeen
      541 vtlkdlkagr hvtkvtiqrl ylmmgrelep tekitagnvf gigdleehvl ktatlsttia
      601 cpsfseltsl gvpimrvale pkhsndlqal inglkllnqa dacalvhiqe sgeivlstag
      661 evhlercled lrvryakvev nvsepivqfr etvvpppkvd mvneaiekkv eegsfsawta
      721 nrqcyfevda rplpdrvtki leknvnlika lyyyygksss eregetkkdk eigktesmys
      781 mserrqrele sfkselaiaf eeagqkdvld riwsfgprnc gsnillnetd ykqqkfwegh
      841 pksidprapy esglmngfql atlagplcee pmmgvcfvvk kweihqdsds ensgqnqsqg
      901 ggylmstfke acrrvfnsrh prlvtpmytc svlvnsdvlg klyaafgkrh grviaadsal
      961 gfggqfrvla tlpvpesfql arelrtqtsg laspqlvfsh wevikqdpyw tpstdeeylh
     1021 fgdkadsenr akkyidavrr rkglpvdsql vthaekqrtl sknk
//
LOCUS       XP_034192076             883 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609612 [Osmia lignaria lignaria].
ACCESSION   XP_034192076
VERSION     XP_034192076.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336185.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..883
                     /product="uncharacterized protein LOC117609612"
                     /calculated_mol_wt=100881
     CDS             1..883
                     /gene="LOC117609612"
                     /coded_by="XM_034336185.2:313..2964"
                     /db_xref="GeneID:117609612"
ORIGIN      
        1 mnkalfdnyt tttkmnddki keeyashnre ldqkyfvaws pleenkrlfk qkyiscsrsv
       61 prrnkrfiqy risrslnfda nihssnsens sfeedkhlsr sakimralnf nssssyygrt
      121 kikkslnfnl tpspkrfmpt kksirktlsl nfnsplsvsn kclnfddnpn nsadnsllys
      181 ssesidenqn etplqqnpke cgilhystpn tklnkrslcs tgpslrsqlr etidnivcit
      241 vtpnsqslkr tkgrldevts tsrnlfhefy dnnddrpstp envidiipes tsaikrshkk
      301 erssrrkdsr ytehtdsled sadlysstdl knsplcnsgn vsdtgslfdy aeeeensfke
      361 sgeasncdni lfeehkfdin eikseesaen sleiesinen ksfekcedvd kydlpdqdid
      421 ikfdirsatp eataevises qtsvtpenri nflqnvlkds ikkshkkikd dnkkklfspk
      481 flrskfetvn skqdeseets vehreeract pervnssrll lsqfssvkks hkkdkhnkil
      541 sgflkrqeyf nkemdlcqre adnsfeckns tadvssdfdn tenvkdaasf tsvhdtplik
      601 lsprksrrsl nvsldcepnv sydsdlqead sskeefkift plkrkrstdg sgvkeylhfy
      661 dlpsgknevs edlavdigls rcltpipnfr dnyiktdedd meakkcdstd vngrstprdm
      721 pttelyvnid sikrshkknk ransswkgfg lfnndsymee qalvksikve alessndcit
      781 vddvnesidh dgqlevstdr icneerasvs tklvpsnvtp pnclksknyl rliqetsikr
      841 shkkvrdkkk qelivdanel sddgsifgde eklacdedqs ikd
//
LOCUS       XP_034192088             820 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609618 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192088
VERSION     XP_034192088.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336197.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192088.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="uncharacterized protein LOC117609618 isoform X1"
                     /calculated_mol_wt=93656
     Region          <416..602
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..820
                     /gene="LOC117609618"
                     /coded_by="XM_034336197.2:267..2729"
                     /db_xref="GeneID:117609618"
ORIGIN      
        1 mdmekhddap kdvkraapve reinkgqkdd tfegypqvve kkkkwehcdk ppskkskhsa
       61 ksdrdccqqr kekrqkaaky imisvpekpc vlpkldivpr hcltkqkyid mlatpsrkcp
      121 pecyskpvlr klkpvsprik elaqptrqrm lmalqqgaat lppafldnlv rtlenetclt
      181 peqaavasrk kkhqkkakgr kskrlfketr ksrkltigvt stgstfdkdp vscqyliaer
      241 fvrsilkwkc pipkaefndi skviiqrlid tleytppqde drksqqlrfl adaiacwvtg
      301 vlsevaenqe kkliercrkk eeemkgdede ddddddrgde dkgkdqleed edeedeeded
      361 ltdrsgksed rereksreev veemeemeee iedesgaevi detdireteg eeqaetqvee
      421 qdlgiqtesk veegtepqae vtaeaeteae gpatadaave vdtiakeetd keqqltaelv
      481 pepepepete viteppeeae vkpeielttk pseelpvepi veeteeqpae seepvpevge
      541 teeitdegai pteeldasvp teeeekekee vealeteegg epleteegkk eesitaletk
      601 apsemglpkd vmkdlvelfk tdlpfltfgk iidtinkmia etpentgedp itngihraiy
      661 eklrnivmle npellteele nvmnvvcgki anwlrsilsk sqlafmkqfv peveskeird
      721 wtkwlsyisd vakdwngwlr dvidkmfeme sapitrgewr dwtksvdtka llwrrfhlqi
      781 rhqahrnvtm isgrhvvktg tkkpisadqs eqlfatdlhi
//
LOCUS       XP_034192096             820 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609618 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192096
VERSION     XP_034192096.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336205.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192096.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..820
                     /product="uncharacterized protein LOC117609618 isoform X1"
                     /calculated_mol_wt=93656
     Region          <416..602
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..820
                     /gene="LOC117609618"
                     /coded_by="XM_034336205.2:261..2723"
                     /db_xref="GeneID:117609618"
ORIGIN      
        1 mdmekhddap kdvkraapve reinkgqkdd tfegypqvve kkkkwehcdk ppskkskhsa
       61 ksdrdccqqr kekrqkaaky imisvpekpc vlpkldivpr hcltkqkyid mlatpsrkcp
      121 pecyskpvlr klkpvsprik elaqptrqrm lmalqqgaat lppafldnlv rtlenetclt
      181 peqaavasrk kkhqkkakgr kskrlfketr ksrkltigvt stgstfdkdp vscqyliaer
      241 fvrsilkwkc pipkaefndi skviiqrlid tleytppqde drksqqlrfl adaiacwvtg
      301 vlsevaenqe kkliercrkk eeemkgdede ddddddrgde dkgkdqleed edeedeeded
      361 ltdrsgksed rereksreev veemeemeee iedesgaevi detdireteg eeqaetqvee
      421 qdlgiqtesk veegtepqae vtaeaeteae gpatadaave vdtiakeetd keqqltaelv
      481 pepepepete viteppeeae vkpeielttk pseelpvepi veeteeqpae seepvpevge
      541 teeitdegai pteeldasvp teeeekekee vealeteegg epleteegkk eesitaletk
      601 apsemglpkd vmkdlvelfk tdlpfltfgk iidtinkmia etpentgedp itngihraiy
      661 eklrnivmle npellteele nvmnvvcgki anwlrsilsk sqlafmkqfv peveskeird
      721 wtkwlsyisd vakdwngwlr dvidkmfeme sapitrgewr dwtksvdtka llwrrfhlqi
      781 rhqahrnvtm isgrhvvktg tkkpisadqs eqlfatdlhi
//
LOCUS       XP_034192106             709 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609618 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192106
VERSION     XP_034192106.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336215.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192106.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..709
                     /product="uncharacterized protein LOC117609618 isoform X2"
                     /calculated_mol_wt=80716
     Region          <305..491
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..709
                     /gene="LOC117609618"
                     /coded_by="XM_034336215.2:171..2300"
                     /db_xref="GeneID:117609618"
ORIGIN      
        1 mlpcsrkcpp ecyskpvlrk lkpvsprike laqptrqrml malqqgaatl ppafldnlvr
       61 tlenetcltp eqaavasrkk khqkkakgrk skrlfketrk srkltigvts tgstfdkdpv
      121 scqyliaerf vrsilkwkcp ipkaefndis kviiqrlidt leytppqded rksqqlrfla
      181 daiacwvtgv lsevaenqek kliercrkke eemkgdeded dddddrgded kgkdqleede
      241 deedeededl tdrsgksedr ereksreevv eemeemeeei edesgaevid etdiretege
      301 eqaetqveeq dlgiqteskv eegtepqaev taeaeteaeg patadaavev dtiakeetdk
      361 eqqltaelvp epepepetev iteppeeaev kpeielttkp seelpvepiv eeteeqpaes
      421 eepvpevget eeitdegaip teeldasvpt eeeekekeev ealeteegge pleteegkke
      481 esitaletka psemglpkdv mkdlvelfkt dlpfltfgki idtinkmiae tpentgedpi
      541 tngihraiye klrnivmlen pellteelen vmnvvcgkia nwlrsilsks qlafmkqfvp
      601 eveskeirdw tkwlsyisdv akdwngwlrd vidkmfemes apitrgewrd wtksvdtkal
      661 lwrrfhlqir hqahrnvtmi sgrhvvktgt kkpisadqse qlfatdlhi
//
LOCUS       XP_034192115             706 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609618 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192115
VERSION     XP_034192115.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336224.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192115.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..706
                     /product="uncharacterized protein LOC117609618 isoform X3"
                     /calculated_mol_wt=80429
     Region          <302..488
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..706
                     /gene="LOC117609618"
                     /coded_by="XM_034336224.2:135..2255"
                     /db_xref="GeneID:117609618"
ORIGIN      
        1 mirkcppecy skpvlrklkp vsprikelaq ptrqrmlmal qqgaatlppa fldnlvrtle
       61 netcltpeqa avasrkkkhq kkakgrkskr lfketrksrk ltigvtstgs tfdkdpvscq
      121 yliaerfvrs ilkwkcpipk aefndiskvi iqrlidtley tppqdedrks qqlrfladai
      181 acwvtgvlse vaenqekkli ercrkkeeem kgdededddd ddrgdedkgk dqleededee
      241 deededltdr sgksedrere ksreevveem eemeeeiede sgaevidetd iretegeeqa
      301 etqveeqdlg iqteskveeg tepqaevtae aeteaegpat adaavevdti akeetdkeqq
      361 ltaelvpepe pepetevite ppeeaevkpe ielttkpsee lpvepiveet eeqpaeseep
      421 vpevgeteei tdegaiptee ldasvpteee ekekeeveal eteeggeple teegkkeesi
      481 taletkapse mglpkdvmkd lvelfktdlp fltfgkiidt inkmiaetpe ntgedpitng
      541 ihraiyeklr nivmlenpel lteelenvmn vvcgkianwl rsilsksqla fmkqfvpeve
      601 skeirdwtkw lsyisdvakd wngwlrdvid kmfemesapi trgewrdwtk svdtkallwr
      661 rfhlqirhqa hrnvtmisgr hvvktgtkkp isadqseqlf atdlhi
//
LOCUS       XP_034192128             704 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609636 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192128
VERSION     XP_034192128.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336237.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192128.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..704
                     /product="uncharacterized protein LOC117609636 isoform X1"
                     /calculated_mol_wt=77639
     Region          98..156
                     /region_name="VWC"
                     /note="von Willebrand factor (vWF) type C domain;
                     smart00214"
                     /db_xref="CDD:214564"
     CDS             1..704
                     /gene="LOC117609636"
                     /coded_by="XM_034336237.2:21..2135"
                     /db_xref="GeneID:117609636"
ORIGIN      
        1 msvfqktaem fyresltvle eitkgierwq wvgyqcstpi aapftnrsfr vsgvdasrck
       61 lvclfertht vqghlgvkee flryavirae sgvsckdqqg rsyesgflfv pgpepctlcv
      121 cengnpklck allcnsraee dckssrrgdi cceyiclssi sndkndgmgs nttdatanyd
      181 iglrsvasfv tavlslsllf flihrlrqrk irvciagrqs rqlaedqrnl gsmgyierdg
      241 lshgvpmddi pcganyplwk ppsnyfprge apppyeeava aaraeqalla mnpqtlpqin
      301 fpntyltnan srtsislvgn aqtglnpnta tvmcdtgvtd qgglisapnr pisnpniyac
      361 yrqpnqnetl spsvnvgtst tsftlgtgty enlpitpigi lnftthrldv taqpmsnlqp
      421 tipksyqsht tlprqtgaft isatlsnsnv nahrtiprti ttrsgtklqe iindctqkef
      481 lrsttstttt tttmtmtmav ttttatatti tnivpsnlsh svqqiaitkl entrenasqg
      541 tfyegvqiqr spvsvtssvg lqqierqqld rkieksqdfk pplnvanein eegsfesvtc
      601 acsmqalptl hddtddyrse cencksatgs ryyldnedel vtsphetmtl hrrpdettss
      661 ttpqyyrtsl tlpistrqrt rstgvrenwf ntmpqsstes sden
//
LOCUS       XP_034192137             700 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609636 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192137
VERSION     XP_034192137.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336246.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192137.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..700
                     /product="uncharacterized protein LOC117609636 isoform X2"
                     /calculated_mol_wt=77253
     Region          98..156
                     /region_name="VWC"
                     /note="von Willebrand factor (vWF) type C domain;
                     smart00214"
                     /db_xref="CDD:214564"
     CDS             1..700
                     /gene="LOC117609636"
                     /coded_by="XM_034336246.2:21..2123"
                     /db_xref="GeneID:117609636"
ORIGIN      
        1 msvfqktaem fyresltvle eitkgierwq wvgyqcstpi aapftnrsfr vsgvdasrck
       61 lvclfertht vqghlgvkee flryavirae sgvsckdqqg rsyesgflfv pgpepctlcv
      121 cengnpklck allcnsraee dckssrrgdi cceyiclssi sndkndgmgs nttdatanyd
      181 iglrsvasfv tavlslsllf flihrlrqrk irgrqsrqla edqrnlgsmg yierdglshg
      241 vpmddipcga nyplwkppsn yfprgeappp yeeavaaara eqallamnpq tlpqinfpnt
      301 yltnansrts islvgnaqtg lnpntatvmc dtgvtdqggl isapnrpisn pniyacyrqp
      361 nqnetlspsv nvgtsttsft lgtgtyenlp itpigilnft thrldvtaqp msnlqptipk
      421 syqshttlpr qtgaftisat lsnsnvnahr tiprtittrs gtklqeiind ctqkeflrst
      481 tstttttttm tmtmavtttt atattitniv psnlshsvqq iaitklentr enasqgtfye
      541 gvqiqrspvs vtssvglqqi erqqldrkie ksqdfkppln vaneineegs fesvtcacsm
      601 qalptlhddt ddyrsecenc ksatgsryyl dnedelvtsp hetmtlhrrp dettssttpq
      661 yyrtsltlpi strqrtrstg vrenwfntmp qsstessden
//
LOCUS       XP_034192142             640 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609636 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192142
VERSION     XP_034192142.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336251.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192142.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..640
                     /product="uncharacterized protein LOC117609636 isoform X3"
                     /calculated_mol_wt=70234
     Region          34..92
                     /region_name="VWC"
                     /note="von Willebrand factor (vWF) type C domain;
                     smart00214"
                     /db_xref="CDD:214564"
     CDS             1..640
                     /gene="LOC117609636"
                     /coded_by="XM_034336251.2:350..2272"
                     /db_xref="GeneID:117609636"
ORIGIN      
        1 mkysdsnllg yavfafilff nviraesgvs ckdqqgrsye sgflfvpgpe pctlcvceng
       61 npklckallc nsraeedcks srrgdiccey iclssisndk ndgmgsnttd atanydiglr
      121 svasfvtavl slsllfflih rlrqrkirvc iagrqsrqla edqrnlgsmg yierdglshg
      181 vpmddipcga nyplwkppsn yfprgeappp yeeavaaara eqallamnpq tlpqinfpnt
      241 yltnansrts islvgnaqtg lnpntatvmc dtgvtdqggl isapnrpisn pniyacyrqp
      301 nqnetlspsv nvgtsttsft lgtgtyenlp itpigilnft thrldvtaqp msnlqptipk
      361 syqshttlpr qtgaftisat lsnsnvnahr tiprtittrs gtklqeiind ctqkeflrst
      421 tstttttttm tmtmavtttt atattitniv psnlshsvqq iaitklentr enasqgtfye
      481 gvqiqrspvs vtssvglqqi erqqldrkie ksqdfkppln vaneineegs fesvtcacsm
      541 qalptlhddt ddyrsecenc ksatgsryyl dnedelvtsp hetmtlhrrp dettssttpq
      601 yyrtsltlpi strqrtrstg vrenwfntmp qsstessden
//
LOCUS       XP_034192153             586 aa            linear   INV 03-SEP-2025
DEFINITION  caseinolytic protease chaperone subunit isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192153
VERSION     XP_034192153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336262.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="caseinolytic protease chaperone subunit isoform
                     X1"
                     /calculated_mol_wt=64026
     Region          72..102
                     /region_name="Zn-ribbon"
                     /note="C-terminal zinc ribbon domain of RNA polymerase
                     intrinsic transcript cleavage subunit; cl02609"
                     /db_xref="CDD:445850"
     Site            order(75,78,97,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259791"
     Region          140..571
                     /region_name="ClpX"
                     /note="ATP-dependent protease Clp, ATPase subunit ClpX
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1219"
                     /db_xref="CDD:440832"
     CDS             1..586
                     /gene="ClpX"
                     /coded_by="XM_034336262.2:728..2488"
                     /db_xref="GeneID:117609654"
ORIGIN      
        1 mscvrcclis agriassnhv anhvhkivrv avirsltiss tlgkaptept ppgkdssggv
       61 ssvgsnggkk ntltcpkcgd pcthvetfvs strfvkcekc hhffvvlsev dskrslkeal
      121 rtddtkqgfy rkpppppkki feylnkhvvg qeyakkvlsv avynhykriy nnfpvqnsmq
      181 gsinttgtqd lnhpfthrgl kphllhistv gnslgvgfqq fptgseqkst dnqsssgsdi
      241 ldskqhqlkl eksnilllgp tgsgktllaq tiaqcldvpf aicdcttltq agyvgedies
      301 viakllqdan yvvdraqmgi vfldevdkig avpgihqlrd vggegvqqgm lkmlegtivn
      361 vpernssrkl rgdtlqvdtt nilfvasgay ngldrlisrr ktekylgfga tpasespgrr
      421 aanladvanm tpstekdnqe kdlllkqvea rdlidfgmip efvgrfpvlv pfhtldrdml
      481 vriltepqna mvpqyqmlfs mdkveltfta dalnaiasla mekktgargl raimesllle
      541 pmfevpgsdv msvhvtegcv rgqdkpqyvk rgdvtdeelq aqqiqn
//
LOCUS       XP_034192160             586 aa            linear   INV 03-SEP-2025
DEFINITION  caseinolytic protease chaperone subunit isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192160
VERSION     XP_034192160.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336269.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="caseinolytic protease chaperone subunit isoform
                     X1"
                     /calculated_mol_wt=64026
     Region          72..102
                     /region_name="Zn-ribbon"
                     /note="C-terminal zinc ribbon domain of RNA polymerase
                     intrinsic transcript cleavage subunit; cl02609"
                     /db_xref="CDD:445850"
     Site            order(75,78,97,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259791"
     Region          140..571
                     /region_name="ClpX"
                     /note="ATP-dependent protease Clp, ATPase subunit ClpX
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1219"
                     /db_xref="CDD:440832"
     CDS             1..586
                     /gene="ClpX"
                     /coded_by="XM_034336269.2:231..1991"
                     /db_xref="GeneID:117609654"
ORIGIN      
        1 mscvrcclis agriassnhv anhvhkivrv avirsltiss tlgkaptept ppgkdssggv
       61 ssvgsnggkk ntltcpkcgd pcthvetfvs strfvkcekc hhffvvlsev dskrslkeal
      121 rtddtkqgfy rkpppppkki feylnkhvvg qeyakkvlsv avynhykriy nnfpvqnsmq
      181 gsinttgtqd lnhpfthrgl kphllhistv gnslgvgfqq fptgseqkst dnqsssgsdi
      241 ldskqhqlkl eksnilllgp tgsgktllaq tiaqcldvpf aicdcttltq agyvgedies
      301 viakllqdan yvvdraqmgi vfldevdkig avpgihqlrd vggegvqqgm lkmlegtivn
      361 vpernssrkl rgdtlqvdtt nilfvasgay ngldrlisrr ktekylgfga tpasespgrr
      421 aanladvanm tpstekdnqe kdlllkqvea rdlidfgmip efvgrfpvlv pfhtldrdml
      481 vriltepqna mvpqyqmlfs mdkveltfta dalnaiasla mekktgargl raimesllle
      541 pmfevpgsdv msvhvtegcv rgqdkpqyvk rgdvtdeelq aqqiqn
//
LOCUS       XP_034192166             586 aa            linear   INV 03-SEP-2025
DEFINITION  caseinolytic protease chaperone subunit isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192166
VERSION     XP_034192166.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336275.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="caseinolytic protease chaperone subunit isoform
                     X1"
                     /calculated_mol_wt=64026
     Region          72..102
                     /region_name="Zn-ribbon"
                     /note="C-terminal zinc ribbon domain of RNA polymerase
                     intrinsic transcript cleavage subunit; cl02609"
                     /db_xref="CDD:445850"
     Site            order(75,78,97,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259791"
     Region          140..571
                     /region_name="ClpX"
                     /note="ATP-dependent protease Clp, ATPase subunit ClpX
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1219"
                     /db_xref="CDD:440832"
     CDS             1..586
                     /gene="ClpX"
                     /coded_by="XM_034336275.2:431..2191"
                     /db_xref="GeneID:117609654"
ORIGIN      
        1 mscvrcclis agriassnhv anhvhkivrv avirsltiss tlgkaptept ppgkdssggv
       61 ssvgsnggkk ntltcpkcgd pcthvetfvs strfvkcekc hhffvvlsev dskrslkeal
      121 rtddtkqgfy rkpppppkki feylnkhvvg qeyakkvlsv avynhykriy nnfpvqnsmq
      181 gsinttgtqd lnhpfthrgl kphllhistv gnslgvgfqq fptgseqkst dnqsssgsdi
      241 ldskqhqlkl eksnilllgp tgsgktllaq tiaqcldvpf aicdcttltq agyvgedies
      301 viakllqdan yvvdraqmgi vfldevdkig avpgihqlrd vggegvqqgm lkmlegtivn
      361 vpernssrkl rgdtlqvdtt nilfvasgay ngldrlisrr ktekylgfga tpasespgrr
      421 aanladvanm tpstekdnqe kdlllkqvea rdlidfgmip efvgrfpvlv pfhtldrdml
      481 vriltepqna mvpqyqmlfs mdkveltfta dalnaiasla mekktgargl raimesllle
      541 pmfevpgsdv msvhvtegcv rgqdkpqyvk rgdvtdeelq aqqiqn
//
LOCUS       XP_034192174             582 aa            linear   INV 03-SEP-2025
DEFINITION  caseinolytic protease chaperone subunit isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192174
VERSION     XP_034192174.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336283.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..582
                     /product="caseinolytic protease chaperone subunit isoform
                     X2"
                     /calculated_mol_wt=63609
     Region          72..102
                     /region_name="Zn-ribbon"
                     /note="C-terminal zinc ribbon domain of RNA polymerase
                     intrinsic transcript cleavage subunit; cl02609"
                     /db_xref="CDD:445850"
     Site            order(75,78,97,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259791"
     Region          140..567
                     /region_name="ClpX"
                     /note="ATP-dependent protease Clp, ATPase subunit ClpX
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1219"
                     /db_xref="CDD:440832"
     CDS             1..582
                     /gene="ClpX"
                     /coded_by="XM_034336283.2:201..1949"
                     /db_xref="GeneID:117609654"
ORIGIN      
        1 mscvrcclis agriassnhv anhvhkivrv avirsltiss tlgkaptept ppgkdssggv
       61 ssvgsnggkk ntltcpkcgd pcthvetfvs strfvkcekc hhffvvlsev dskrslkeal
      121 rtddtkqgfy rkpppppkki feylnkhvvg qeyakkvlsv avynhykriy nnfpvqnsmq
      181 gsinttgtqd lnhpfthrdl lhistvgnsl gvgfqqfptg seqkstdnqs ssgsdildsk
      241 qhqlkleksn illlgptgsg ktllaqtiaq cldvpfaicd cttltqagyv gediesviak
      301 llqdanyvvd raqmgivfld evdkigavpg ihqlrdvgge gvqqgmlkml egtivnvper
      361 nssrklrgdt lqvdttnilf vasgayngld rlisrrktek ylgfgatpas espgrraanl
      421 advanmtpst ekdnqekdll lkqveardli dfgmipefvg rfpvlvpfht ldrdmlvril
      481 tepqnamvpq yqmlfsmdkv eltftadaln aiaslamekk tgarglraim eslllepmfe
      541 vpgsdvmsvh vtegcvrgqd kpqyvkrgdv tdeelqaqqi qn
//
LOCUS       XP_034192176             564 aa            linear   INV 03-SEP-2025
DEFINITION  caseinolytic protease chaperone subunit isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192176
VERSION     XP_034192176.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336285.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="caseinolytic protease chaperone subunit isoform
                     X3"
                     /calculated_mol_wt=61631
     Region          72..102
                     /region_name="Zn-ribbon"
                     /note="C-terminal zinc ribbon domain of RNA polymerase
                     intrinsic transcript cleavage subunit; cl02609"
                     /db_xref="CDD:445850"
     Site            order(75,78,97,100)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:259791"
     Region          140..549
                     /region_name="ClpX"
                     /note="ATP-dependent protease Clp, ATPase subunit ClpX
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1219"
                     /db_xref="CDD:440832"
     CDS             1..564
                     /gene="ClpX"
                     /coded_by="XM_034336285.2:201..1895"
                     /db_xref="GeneID:117609654"
ORIGIN      
        1 mscvrcclis agriassnhv anhvhkivrv avirsltiss tlgkaptept ppgkdssggv
       61 ssvgsnggkk ntltcpkcgd pcthvetfvs strfvkcekc hhffvvlsev dskrslkeal
      121 rtddtkqgfy rkpppppkki feylnkhvvg qeyakkvlsv avynhykriy nnfpvqnsmq
      181 dllhistvgn slgvgfqqfp tgseqkstdn qsssgsdild skqhqlklek snilllgptg
      241 sgktllaqti aqcldvpfai cdcttltqag yvgediesvi akllqdanyv vdraqmgivf
      301 ldevdkigav pgihqlrdvg gegvqqgmlk mlegtivnvp ernssrklrg dtlqvdttni
      361 lfvasgayng ldrlisrrkt ekylgfgatp asespgrraa nladvanmtp stekdnqekd
      421 lllkqveard lidfgmipef vgrfpvlvpf htldrdmlvr iltepqnamv pqyqmlfsmd
      481 kveltftada lnaiaslame kktgarglra imeslllepm fevpgsdvms vhvtegcvrg
      541 qdkpqyvkrg dvtdeelqaq qiqn
//
LOCUS       XP_034192181             555 aa            linear   INV 03-SEP-2025
DEFINITION  de-etiolated protein 1 abo [Osmia lignaria lignaria].
ACCESSION   XP_034192181
VERSION     XP_034192181.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336290.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192181.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..555
                     /product="de-etiolated protein 1 abo"
                     /calculated_mol_wt=64000
     Region          152..554
                     /region_name="Det1"
                     /note="De-etiolated protein 1 Det1; pfam09737"
                     /db_xref="CDD:462868"
     CDS             1..555
                     /gene="abo"
                     /coded_by="XM_034336290.2:606..2273"
                     /db_xref="GeneID:117609675"
ORIGIN      
        1 maekeakiey vtepcpikpr kigpqnvvir lkrreifgcp ypgihvhnar qfyqnvfpnf
       61 tvmnvekppc flrkfspdgr yliafsadqt sievyeyrga saaadllanc egeyighknd
      121 eysfqirsni fsrffkakwi vnvvqsneql nrecslftdd gryvivgsaa hipdelrphf
      181 yqiytnneal tpnprspled yslhlvdlrg gklcdtrhfk vdkiylshnq glylykdila
      241 vlsvqhqtih ifqildgmfi nvrtigrfcl eddaylvtsa cpgvncrpfr dvtinslkhk
      301 llvylykraa yisettkdpy elrrfyqyfd qlnalrmwkm qlldtnhilv rfaseevatl
      361 qanepnaqpa llvvydmvsa kilaaydnas tqlltqfenf sdffrnarms tdcqymcsps
      421 nniyarllqq rfkqtivsar yggvteatkr llaqlpicaq syssspyldl slfcyddkwv
      481 smmerpkacg ehpirfyard sgllkfrmya gmlgrttpta arrlvaftfh ptdpfaisvq
      541 rtnaeyivsf hvrhv
//
LOCUS       XP_034192191             462 aa            linear   INV 03-SEP-2025
DEFINITION  RIO kinase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034192191
VERSION     XP_034192191.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336300.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192191.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="RIO kinase 2"
                     /calculated_mol_wt=53156
     Region          9..91
                     /region_name="Rio2_N"
                     /note="Rio2, N-terminal; pfam09202"
                     /db_xref="CDD:462715"
     Region          94..276
                     /region_name="RIO2_C"
                     /note="C-terminal catalytic domain of the atypical protein
                     serine kinase, RIO2 kinase; cd05144"
                     /db_xref="CDD:270695"
     Site            order(101,106..107,109,121,123,176,188..191,195,232..233,
                     245..246)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270695"
     CDS             1..462
                     /gene="RIOK2"
                     /coded_by="XM_034336300.2:213..1601"
                     /db_xref="GeneID:117609684"
ORIGIN      
        1 mgklnvtilr yltrddfrvl taiemgmknh elvpaslaaq ianlryggvh kfmkelckhr
       61 llsyergkhy dgyrltnagy dylalkvlaq rgtvssfgnq igvgkesniy ivadeegnpv
      121 clklhrlgrt cfrnikdkrd yhqhrksasw lylsrisatr efaymkalyd rgfpipkpid
      181 lnrhcvvmel veggplcgvy evddiewlyd elmnlivklg nhgvihgdfn efnimitssg
      241 kpilidfpqm vstehadaki yferdvncvr dffkrrfaye selyptfedi lredcidvei
      301 kasgltkqme kdllremgmi eseaeeaeee seenveeqke smdeseinyl qqqvedcvrn
      361 eyiytpkdvs vdaetneatf gseddqvqtg efsdsksmys tttvstiape vikkkvksal
      421 qkrekreqsr rilvkgeana vtrvrrenkd tikqstgiwg we
//
LOCUS       XP_034192196             452 aa            linear   INV 03-SEP-2025
DEFINITION  COUP transcription factor 2 [Osmia lignaria lignaria].
ACCESSION   XP_034192196
VERSION     XP_034192196.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336305.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="COUP transcription factor 2"
                     /calculated_mol_wt=48782
     Region          109..181
                     /region_name="NR_DBD_COUP_TF"
                     /note="DNA-binding domain of chicken ovalbumin upstream
                     promoter transcription factors (COUP-TFs) is composed of
                     two C4-type zinc fingers; cd06958"
                     /db_xref="CDD:143516"
     Site            order(109,112,126,129,145,151,161,164)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143516"
     Site            order(118..121,127..128,130,132,135,138,146,158..159,162,
                     165,176)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143516"
     Region          216..451
                     /region_name="NR_LBD_COUP-TF"
                     /note="Ligand binding domain of chicken ovalbumin upstream
                     promoter transcription factors, a member of the nuclear
                     receptor family; cd06948"
                     /db_xref="CDD:132746"
     Site            order(249..250,254,288,292,295,309,330)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:132746"
     Site            order(259,262,266,271,276..277,279..280,283..284)
                     /site_type="active"
                     /note="putative coactivator recognition site [active]"
                     /db_xref="CDD:132746"
     CDS             1..452
                     /gene="svp"
                     /coded_by="XM_034336305.2:1009..2367"
                     /db_xref="GeneID:117609691"
ORIGIN      
        1 malvaappsw psrdpgasla ataaaaaaga aavaastagp gpiqiapppp mppthltpsa
       61 tpeditclvp agavltprdp lppsttpgsq anssqsgssq tdkspniecv vcgdkssgkh
      121 ygqftcegck sffkrsvrrn ltyscrgnrn cpidqhhrnq cqfcrlkkcl kmgmrreavq
      181 rgrvppsqps lpglpgqfal tngdavacas lnghsylssy islllraepy ptsrygqcmq
      241 pnnimgidni celaarllfs avewarnipf fpdlqvtdqv allrlvwsel fvlnasqcsm
      301 plhvapllaa aglhaspmaa drvvafmdhi rifqeqvekl kalhvdsaey sclkaivlft
      361 tdacglsdva hieslqeksq caleeycrtq ypnqptrfgk lllrlpslrt vssqvieqlf
      421 fvrlvgktpi etlirdmlls gssfnwpyms tm
//
LOCUS       XP_034192206             449 aa            linear   INV 03-SEP-2025
DEFINITION  doublesex cognate 73A [Osmia lignaria lignaria].
ACCESSION   XP_034192206
VERSION     XP_034192206.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336315.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192206.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="doublesex cognate 73A"
                     /calculated_mol_wt=48433
     CDS             1..449
                     /gene="dsx-c73A"
                     /coded_by="XM_034336315.2:479..1828"
                     /db_xref="GeneID:117609697"
ORIGIN      
        1 mqpiqggkwf vlmivgsmal ppqsqtgkgk etmpvtgnda hfdpsstiss ntsnaivasn
       61 sannsvdidi nddsgstvsk pvsldvsapi gndettlass svtpilenrs spdeivensp
      121 ttrssiagae ststttttta ttksnvvtna kttmingnns ntgssvapte tssrtivstq
      181 dastflsssk kstssfnest ttpasisake trespgkirp qiklttnyts ltetgvrlpe
      241 gasaalakpt kyhyyphnqh iyllpecavq qvcnavyvrl nftqplcacp gryrdpcsas
      301 ldsddlhtte lvtdprtkal tlvktcepva emrecraprd wsllalqnvr tgkshylvic
      361 rcpdanileg pmshdqptya svpgirvygm mcvqrtrrgr plrnvrslid spiaeindns
      421 mpkenpetnd rlsfpwykvq qlmntavwd
//
LOCUS       XP_034192225             398 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial outer membrane protein SLC25A46 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192225
VERSION     XP_034192225.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336334.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..398
                     /product="mitochondrial outer membrane protein SLC25A46"
                     /calculated_mol_wt=44488
     Region          55..>130
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     Region          281..>356
                     /region_name="Mito_carr"
                     /note="Mitochondrial carrier protein; pfam00153"
                     /db_xref="CDD:395101"
     CDS             1..398
                     /gene="LOC117609710"
                     /coded_by="XM_034336334.2:131..1327"
                     /db_xref="GeneID:117609710"
ORIGIN      
        1 maglesyerv yrgksikswe tennlayiyq grevirpldi pavhhlaeds ppseedlsvk
       61 kymvagcgla tliaetllvh plivlrrqcq vnpglksyhi vpitlipvvi rlhqtqgiht
      121 lwkgigsvll vkgmllaved fiskitpwpk eitcnsslka fgqhillkcv siglvtpfys
      181 aslvetvqse iasespgild vfrdgairlv dvsnkgrlip iyallpptia ykvskylftl
      241 avrgvtsrim hirhkhsqei rgaysrdlls eatvqdielq salvstfiae alfypwqtvi
      301 yrlhlqgtrt iidnldtgrs vtplltgysg ardcystiis tegplglykg fgafileftv
      361 divivrvikw ivteletilr pkpksirdpp qhwyynet
//
LOCUS       XP_034192237             393 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609715 [Osmia lignaria lignaria].
ACCESSION   XP_034192237
VERSION     XP_034192237.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336346.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192237.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="uncharacterized protein LOC117609715"
                     /calculated_mol_wt=45411
     Region          <340..391
                     /region_name="BUD22"
                     /note="pfam09073"
                     /db_xref="CDD:401132"
     CDS             1..393
                     /gene="LOC117609715"
                     /coded_by="XM_034336346.2:22..1203"
                     /db_xref="GeneID:117609715"
ORIGIN      
        1 mrqteinnel vllrqpvhqa ricvvsklir eakklrtyrg nekqleknkt ksdkllrevf
       61 alkhikdddi stfgiinfeh lqdilknprt degtramikv arykclheri vefmekfpdy
      121 seyiswkkkk rpskrkgsns idddleenpk rsrkdtntvk vstqkrgnkn idstgttnhl
      181 qcttrqqkki neqsmklske sveqklegee sksvakvisk eaavkrfaev lqetdrkeea
      241 seheennneq ssndagecrk stddfflssd eagssvtvip aernedrtsd idhrafkalr
      301 irskdekfsk dkgeigsdkd isygpsntrk tnvthskqnr dvrvknnpvk rnvesdstkt
      361 edenlhpswv arrkqqeilk qgfqgkkikf ded
//
LOCUS       XP_034192251             373 aa            linear   INV 03-SEP-2025
DEFINITION  sorbitol dehydrogenase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192251
VERSION     XP_034192251.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336360.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192251.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="sorbitol dehydrogenase isoform X1"
                     /calculated_mol_wt=40144
     Region          8..372
                     /region_name="sorbitol_DH"
                     /note="Sorbitol dehydrogenase; cd05285"
                     /db_xref="CDD:176188"
     Site            order(41,43,47,53,56,66,115,118,152,296,319..320)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:176188"
     Site            order(41,43,66..67,152)
                     /site_type="other"
                     /note="catalytic Zn binding site [ion binding]"
                     /db_xref="CDD:176188"
     Site            order(96,99,102,110)
                     /site_type="other"
                     /note="structural Zn binding site [ion binding]"
                     /db_xref="CDD:176188"
     Site            order(156,178..180,199,223..224,228,243,271..272,274,
                     294..296,318..320)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:176188"
     Site            order(158,169,171,187,190..192,194,226,242,258,327,
                     330..331,334)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:176188"
     CDS             1..373
                     /gene="LOC117609724"
                     /coded_by="XM_034336360.2:234..1355"
                     /db_xref="GeneID:117609724"
ORIGIN      
        1 makdnltail ygindirleq tpieepnqde vllqmgcvgi cgsdvhylvs ggigdfvvrk
       61 pmimgheaag vvvklgknvk hlkvgdrvai epgvscrmct fckggrynlc kdmvfcatpp
      121 vhgnlrrfyk haadfcfklp dnvtlaegal leplsvgvha ckranisigs kvlilgagpi
      181 glvtllvaka mgaskivitg tftfknvlfl tfilvinrrs sldlsqnrln makklganat
      241 llikpeyaea dvvakvhqlf ggeepdrtid asgaqssirl ailatksggv avmvgmgaae
      301 vqiplinalv revdirgvfr yandysdald llatqkidvk plithnykie etveafetsk
      361 sgqdvikvmi ycn
//
LOCUS       XP_034192260             350 aa            linear   INV 03-SEP-2025
DEFINITION  sorbitol dehydrogenase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192260
VERSION     XP_034192260.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336369.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="sorbitol dehydrogenase isoform X2"
                     /calculated_mol_wt=37475
     Region          8..349
                     /region_name="sorbitol_DH"
                     /note="Sorbitol dehydrogenase; cd05285"
                     /db_xref="CDD:176188"
     Site            order(41,43,47,53,56,66,115,118,152,273,296..297)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:176188"
     Site            order(41,43,66..67,152)
                     /site_type="other"
                     /note="catalytic Zn binding site [ion binding]"
                     /db_xref="CDD:176188"
     Site            order(96,99,102,110)
                     /site_type="other"
                     /note="structural Zn binding site [ion binding]"
                     /db_xref="CDD:176188"
     Site            order(156,178..180,199..201,205,220,248..249,251,271..273,
                     295..297)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:176188"
     Site            order(158,169,171,187,190..192,194,203,219,235,304,
                     307..308,311)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:176188"
     CDS             1..350
                     /gene="LOC117609724"
                     /coded_by="XM_034336369.2:232..1284"
                     /db_xref="GeneID:117609724"
ORIGIN      
        1 makdnltail ygindirleq tpieepnqde vllqmgcvgi cgsdvhylvs ggigdfvvrk
       61 pmimgheaag vvvklgknvk hlkvgdrvai epgvscrmct fckggrynlc kdmvfcatpp
      121 vhgnlrrfyk haadfcfklp dnvtlaegal leplsvgvha ckranisigs kvlilgagpi
      181 glvtllvaka mgaskivitd lsqnrlnmak klganatlli kpeyaeadvv akvhqlfgge
      241 epdrtidasg aqssirlail atksggvavm vgmgaaevqi plinalvrev dirgvfryan
      301 dysdaldlla tqkidvkpli thnykieetv eafetsksgq dvikvmiycn
//
LOCUS       XP_034192267             326 aa            linear   INV 03-SEP-2025
DEFINITION  very-long-chain 3-oxoacyl-CoA reductase [Osmia lignaria lignaria].
ACCESSION   XP_034192267
VERSION     XP_034192267.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336376.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="very-long-chain 3-oxoacyl-CoA reductase"
                     /calculated_mol_wt=35586
     Region          48..284
                     /region_name="17beta-HSD1_like_SDR_c"
                     /note="17-beta-hydroxysteroid dehydrogenases (17beta-HSDs)
                     types -1, -3, and -12, -like, classical (c) SDRs; cd05356"
                     /db_xref="CDD:187614"
     Site            order(55,57..60,79..81,104..106,132..134,157,184..186,199,
                     203,229..232,234..235)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187614"
     Site            order(158,186,199,203)
                     /site_type="active"
                     /db_xref="CDD:187614"
     CDS             1..326
                     /gene="LOC117609732"
                     /coded_by="XM_034336376.2:151..1131"
                     /db_xref="GeneID:117609732"
ORIGIN      
        1 mtltcwekls lvvlaaiglr illrlslilw rqyigptlgf gidpkdqgrw avitgstsgi
       61 gkayaeqlar kgmdivlvsr slekleevsa eikerygvkv rivdadltkg qvayakvaka
      121 ieeleigvvv nnagasyehp elftqiteec vaqilqlnve aitgiakivl pkmferrkgi
      181 linissllgi mpapyltvyg askayvvkls qdlaveaeph gvtvqcivpa mvatkmskik
      241 kttwmapsae kfvesslkti gielistgyp phylllasvn vlrsicekat iwliaktmcn
      301 vrrralqgka rqqkkaqqqg splaae
//
LOCUS       XP_034192271             326 aa            linear   INV 03-SEP-2025
DEFINITION  very-long-chain 3-oxoacyl-CoA reductase [Osmia lignaria lignaria].
ACCESSION   XP_034192271
VERSION     XP_034192271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336380.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="very-long-chain 3-oxoacyl-CoA reductase"
                     /calculated_mol_wt=35586
     Region          48..284
                     /region_name="17beta-HSD1_like_SDR_c"
                     /note="17-beta-hydroxysteroid dehydrogenases (17beta-HSDs)
                     types -1, -3, and -12, -like, classical (c) SDRs; cd05356"
                     /db_xref="CDD:187614"
     Site            order(55,57..60,79..81,104..106,132..134,157,184..186,199,
                     203,229..232,234..235)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187614"
     Site            order(158,186,199,203)
                     /site_type="active"
                     /db_xref="CDD:187614"
     CDS             1..326
                     /gene="LOC117609732"
                     /coded_by="XM_034336380.2:148..1128"
                     /db_xref="GeneID:117609732"
ORIGIN      
        1 mtltcwekls lvvlaaiglr illrlslilw rqyigptlgf gidpkdqgrw avitgstsgi
       61 gkayaeqlar kgmdivlvsr slekleevsa eikerygvkv rivdadltkg qvayakvaka
      121 ieeleigvvv nnagasyehp elftqiteec vaqilqlnve aitgiakivl pkmferrkgi
      181 linissllgi mpapyltvyg askayvvkls qdlaveaeph gvtvqcivpa mvatkmskik
      241 kttwmapsae kfvesslkti gielistgyp phylllasvn vlrsicekat iwliaktmcn
      301 vrrralqgka rqqkkaqqqg splaae
//
LOCUS       XP_034192283             322 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase Iruka isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192283
VERSION     XP_034192283.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336392.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="E3 ubiquitin-protein ligase Iruka isoform X1"
                     /calculated_mol_wt=35238
     Region          12..43
                     /region_name="zinc_ribbon_9"
                     /note="zinc-ribbon; pfam14369"
                     /db_xref="CDD:433910"
     Region          226..268
                     /region_name="RING-H2_RNF126-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF126, RNF115, and similar proteins; cd16667"
                     /db_xref="CDD:438329"
     CDS             1..322
                     /gene="Iru"
                     /coded_by="XM_034336392.2:622..1590"
                     /db_xref="GeneID:117609745"
ORIGIN      
        1 madavvdgtp msrffchkcs ieierllpdy tcprcasgfi eelesssnds gsgmdissed
       61 lsdiddnppy nfpqrnlyga erdliemllg isnqgqtsna snrnngptgr rrvhwyrnaq
      121 drrrsnslgr reavsvenfi qefvlnlsgv gvaqtvtqpg qlpvfnirlf lgnpgdyvwg
      181 qdgldaivtq llnqldgtgp pplprkqide ipntmvtqth vesklqcsvc wedfklsesv
      241 kqlacqhlyh gpcivpwlel hgtcpicrqn lgdqnsadvn qdtvgpslaa lfraanesns
      301 trtsstsssg gnnssnnsts ei
//
LOCUS       XP_034192288             322 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase Iruka isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192288
VERSION     XP_034192288.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336397.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="E3 ubiquitin-protein ligase Iruka isoform X1"
                     /calculated_mol_wt=35238
     Region          12..43
                     /region_name="zinc_ribbon_9"
                     /note="zinc-ribbon; pfam14369"
                     /db_xref="CDD:433910"
     Region          226..268
                     /region_name="RING-H2_RNF126-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF126, RNF115, and similar proteins; cd16667"
                     /db_xref="CDD:438329"
     CDS             1..322
                     /gene="Iru"
                     /coded_by="XM_034336397.2:630..1598"
                     /db_xref="GeneID:117609745"
ORIGIN      
        1 madavvdgtp msrffchkcs ieierllpdy tcprcasgfi eelesssnds gsgmdissed
       61 lsdiddnppy nfpqrnlyga erdliemllg isnqgqtsna snrnngptgr rrvhwyrnaq
      121 drrrsnslgr reavsvenfi qefvlnlsgv gvaqtvtqpg qlpvfnirlf lgnpgdyvwg
      181 qdgldaivtq llnqldgtgp pplprkqide ipntmvtqth vesklqcsvc wedfklsesv
      241 kqlacqhlyh gpcivpwlel hgtcpicrqn lgdqnsadvn qdtvgpslaa lfraanesns
      301 trtsstsssg gnnssnnsts ei
//
LOCUS       XP_034192295             318 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase Iruka isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192295
VERSION     XP_034192295.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336404.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="E3 ubiquitin-protein ligase Iruka isoform X2"
                     /calculated_mol_wt=34708
     Region          12..43
                     /region_name="zinc_ribbon_9"
                     /note="zinc-ribbon; pfam14369"
                     /db_xref="CDD:433910"
     Region          222..264
                     /region_name="RING-H2_RNF126-like"
                     /note="RING finger, H2 subclass, found in RING finger
                     proteins RNF126, RNF115, and similar proteins; cd16667"
                     /db_xref="CDD:438329"
     CDS             1..318
                     /gene="Iru"
                     /coded_by="XM_034336404.2:792..1748"
                     /db_xref="GeneID:117609745"
ORIGIN      
        1 madavvdgtp msrffchkcs ieierllpdy tcprcasgfi eelesssnds gsgmdissed
       61 lsdiddnppy nfpqrnlyga erdliemllg isnqgqtsna snrnngptgr rrvhwyrnaq
      121 drrrsnslgr reavsvenfi qefvlnlsgv gvaqtvtqpg qlpvlflgnp gdyvwgqdgl
      181 daivtqllnq ldgtgppplp rkqideipnt mvtqthvesk lqcsvcwedf klsesvkqla
      241 cqhlyhgpci vpwlelhgtc picrqnlgdq nsadvnqdtv gpslaalfra anesnstrts
      301 stsssggnns snnstsei
//
LOCUS       XP_034192308             298 aa            linear   INV 03-SEP-2025
DEFINITION  protein argos [Osmia lignaria lignaria].
ACCESSION   XP_034192308
VERSION     XP_034192308.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336417.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="protein argos"
                     /calculated_mol_wt=33598
     Region          134..265
                     /region_name="Argos"
                     /note="Antagonist of EGFR signalling, Argos; pfam11581"
                     /db_xref="CDD:371613"
     CDS             1..298
                     /gene="aos"
                     /coded_by="XM_034336417.2:2890..3786"
                     /db_xref="GeneID:117609760"
ORIGIN      
        1 maeltcllla mfgqllvrgs kgsflgield ktgqkflney lsleegkylg elqpfqdtai
       61 eldlidqkrk hrpmhqpprv lyqigdseee lpecsdrsev cskvdlygsp wverqcrcpa
      121 grscpsslhg ddghtivdkt rqyklcepvk rlpicryfkd vtwtlapggg panatvqrvy
      181 crcrpgsvpy lvrrqayrss egnpgflyaf acspqsrlrc qhkepcrlft vrkrrtsqle
      241 evnasplcqc pkghrcprrh tdpgslpars ysgvleikty sgycvpsqph hseavytv
//
LOCUS       XP_034196265             734 aa            linear   INV 03-SEP-2025
DEFINITION  semaphorin 2a isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196265
VERSION     XP_034196265.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340374.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..734
                     /product="semaphorin 2a isoform X1"
                     /calculated_mol_wt=82801
     Region          70..528
                     /region_name="Sema_2A"
                     /note="The Sema domain, a protein interacting module, of
                     semaphorin 2A (Sema2A); cd11238"
                     /db_xref="CDD:200499"
     Site            order(121..124,182..183,187,215,217..218,225,243,246..247,
                     249,254,276,300..304,306,410,414,417)
                     /site_type="other"
                     /note="putative plexin binding site [polypeptide binding]"
                     /db_xref="CDD:200499"
     Site            order(277..278,280,283..285,319,323..325,327,329,349..351,
                     355..356,423,438)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:200499"
     Region          532..>555
                     /region_name="PSI"
                     /note="Plexin repeat; pfam01437"
                     /db_xref="CDD:396154"
     Region          580..675
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          591..595
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          607..611
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          639..643
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          653..658
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          670..673
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..734
                     /gene="Sema2a"
                     /coded_by="XM_034340374.2:1194..3398"
                     /db_xref="GeneID:117611913"
ORIGIN      
        1 mprarlpdam lllvraavla avlgqflgps hasrpagahp ghayfeqpcc grshlrhhke
       61 hvrefscgml yyrtlyldsk rdalyvgamd kvfrlnlsni shsncerdal nlepsnvanc
      121 vskgksehfd crnhirviqp mgdgnrlymc gtnahspkdw viysnlthlp rhefvpgvgm
      181 giakcpydpa dnstavwvek gnpgdlpaly sgtnaeftka dtvifrtdly nlttgrkafs
      241 fkrtlkydsk wldkpnfvgs ydigshvfff fretaveyin cgksvysrva rvckrdtggk
      301 nilsqnwsty lkarlncsip gefpfyfnei qsiykvpgdd thfygtftts tnglmgsaic
      361 sfhidaiqea frgkfkeqat sssawlpvls nkvpdprpgq cvndtetfpd tvlnfirshp
      421 lmdsaishen ekpvfykrdv mltrlvvdkl ridfvgadld ytvyyagssd grvhkvvqwi
      481 dnngeshsil ldvfdvtpge piqameiske hkalyvasdh rikqidlvmc trrydnclrc
      541 vhdpycgwdk dtntckpyep gllqdvsnnt advcdssvgk rklvvtwgqs vhlgcfvkmp
      601 evlanqevrw yhyskekgry qiaykygagg dkfietsekg lvivgvneqd agrydcwlgg
      661 sllcsynitv dahrcsapak sndyqkiysd wchefekyks amkswerkqa qcasrqndsn
      721 qnlhanevyg tplv
//
LOCUS       XP_034196268             711 aa            linear   INV 03-SEP-2025
DEFINITION  semaphorin 2a isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196268
VERSION     XP_034196268.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340377.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196268.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..711
                     /product="semaphorin 2a isoform X2"
                     /calculated_mol_wt=80677
     Region          47..505
                     /region_name="Sema_2A"
                     /note="The Sema domain, a protein interacting module, of
                     semaphorin 2A (Sema2A); cd11238"
                     /db_xref="CDD:200499"
     Site            order(98..101,159..160,164,192,194..195,202,220,223..224,
                     226,231,253,277..281,283,387,391,394)
                     /site_type="other"
                     /note="putative plexin binding site [polypeptide binding]"
                     /db_xref="CDD:200499"
     Site            order(254..255,257,260..262,296,300..302,304,306,326..328,
                     332..333,400,415)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:200499"
     Region          509..>532
                     /region_name="PSI"
                     /note="Plexin repeat; pfam01437"
                     /db_xref="CDD:396154"
     Region          557..652
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          568..572
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          584..588
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          616..620
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          630..635
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          647..650
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..711
                     /gene="Sema2a"
                     /coded_by="XM_034340377.2:14..2149"
                     /db_xref="GeneID:117611913"
ORIGIN      
        1 mdtsrnrtwl rrmacllwlv flfvgvsagt leklheehvr efscgmlyyr tlyldskrda
       61 lyvgamdkvf rlnlsnishs ncerdalnle psnvancvsk gksehfdcrn hirviqpmgd
      121 gnrlymcgtn ahspkdwviy snlthlprhe fvpgvgmgia kcpydpadns tavwvekgnp
      181 gdlpalysgt naeftkadtv ifrtdlynlt tgrkafsfkr tlkydskwld kpnfvgsydi
      241 gshvffffre taveyincgk svysrvarvc krdtggknil sqnwstylka rlncsipgef
      301 pfyfneiqsi ykvpgddthf ygtfttstng lmgsaicsfh idaiqeafrg kfkeqatsss
      361 awlpvlsnkv pdprpgqcvn dtetfpdtvl nfirshplmd saishenekp vfykrdvmlt
      421 rlvvdklrid fvgadldytv yyagssdgrv hkvvqwidnn geshsilldv fdvtpgepiq
      481 ameiskehka lyvasdhrik qidlvmctrr ydnclrcvhd pycgwdkdtn tckpyepgll
      541 qdvsnntadv cdssvgkrkl vvtwgqsvhl gcfvkmpevl anqevrwyhy skekgryqia
      601 ykygaggdkf ietsekglvi vgvneqdagr ydcwlggsll csynitvdah rcsapaksnd
      661 yqkiysdwch efekyksamk swerkqaqca srqndsnqnl hanevygtpl v
//
LOCUS       XP_034196270             681 aa            linear   INV 03-SEP-2025
DEFINITION  semaphorin 2a isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196270
VERSION     XP_034196270.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340379.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..681
                     /product="semaphorin 2a isoform X3"
                     /calculated_mol_wt=77102
     Region          17..475
                     /region_name="Sema_2A"
                     /note="The Sema domain, a protein interacting module, of
                     semaphorin 2A (Sema2A); cd11238"
                     /db_xref="CDD:200499"
     Site            order(68..71,129..130,134,162,164..165,172,190,193..194,
                     196,201,223,247..251,253,357,361,364)
                     /site_type="other"
                     /note="putative plexin binding site [polypeptide binding]"
                     /db_xref="CDD:200499"
     Site            order(224..225,227,230..232,266,270..272,274,276,296..298,
                     302..303,370,385)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:200499"
     Region          479..>502
                     /region_name="PSI"
                     /note="Plexin repeat; pfam01437"
                     /db_xref="CDD:396154"
     Region          527..622
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          538..542
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          554..558
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          586..590
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          600..605
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          617..620
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..681
                     /gene="Sema2a"
                     /coded_by="XM_034340379.2:74..2119"
                     /db_xref="GeneID:117611913"
ORIGIN      
        1 mdlsviehvr efscgmlyyr tlyldskrda lyvgamdkvf rlnlsnishs ncerdalnle
       61 psnvancvsk gksehfdcrn hirviqpmgd gnrlymcgtn ahspkdwviy snlthlprhe
      121 fvpgvgmgia kcpydpadns tavwvekgnp gdlpalysgt naeftkadtv ifrtdlynlt
      181 tgrkafsfkr tlkydskwld kpnfvgsydi gshvffffre taveyincgk svysrvarvc
      241 krdtggknil sqnwstylka rlncsipgef pfyfneiqsi ykvpgddthf ygtfttstng
      301 lmgsaicsfh idaiqeafrg kfkeqatsss awlpvlsnkv pdprpgqcvn dtetfpdtvl
      361 nfirshplmd saishenekp vfykrdvmlt rlvvdklrid fvgadldytv yyagssdgrv
      421 hkvvqwidnn geshsilldv fdvtpgepiq ameiskehka lyvasdhrik qidlvmctrr
      481 ydnclrcvhd pycgwdkdtn tckpyepgll qdvsnntadv cdssvgkrkl vvtwgqsvhl
      541 gcfvkmpevl anqevrwyhy skekgryqia ykygaggdkf ietsekglvi vgvneqdagr
      601 ydcwlggsll csynitvdah rcsapaksnd yqkiysdwch efekyksamk swerkqaqca
      661 srqndsnqnl hanevygtpl v
//
LOCUS       XP_034196271             666 aa            linear   INV 03-SEP-2025
DEFINITION  semaphorin 2a isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196271
VERSION     XP_034196271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340380.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..666
                     /product="semaphorin 2a isoform X4"
                     /calculated_mol_wt=75398
     Region          2..460
                     /region_name="Sema_2A"
                     /note="The Sema domain, a protein interacting module, of
                     semaphorin 2A (Sema2A); cd11238"
                     /db_xref="CDD:200499"
     Site            order(53..56,114..115,119,147,149..150,157,175,178..179,
                     181,186,208,232..236,238,342,346,349)
                     /site_type="other"
                     /note="putative plexin binding site [polypeptide binding]"
                     /db_xref="CDD:200499"
     Site            order(209..210,212,215..217,251,255..257,259,261,281..283,
                     287..288,355,370)
                     /site_type="other"
                     /note="putative homodimer interface [polypeptide binding]"
                     /db_xref="CDD:200499"
     Region          464..>487
                     /region_name="PSI"
                     /note="Plexin repeat; pfam01437"
                     /db_xref="CDD:396154"
     Region          512..607
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          523..527
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          539..543
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          571..575
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          585..590
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          602..605
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..666
                     /gene="Sema2a"
                     /coded_by="XM_034340380.2:308..2308"
                     /db_xref="GeneID:117611913"
ORIGIN      
        1 mlyyrtlyld skrdalyvga mdkvfrlnls nishsncerd alnlepsnva ncvskgkseh
       61 fdcrnhirvi qpmgdgnrly mcgtnahspk dwviysnlth lprhefvpgv gmgiakcpyd
      121 padnstavwv ekgnpgdlpa lysgtnaeft kadtvifrtd lynlttgrka fsfkrtlkyd
      181 skwldkpnfv gsydigshvf fffretavey incgksvysr varvckrdtg gknilsqnws
      241 tylkarlncs ipgefpfyfn eiqsiykvpg ddthfygtft tstnglmgsa icsfhidaiq
      301 eafrgkfkeq atsssawlpv lsnkvpdprp gqcvndtetf pdtvlnfirs hplmdsaish
      361 enekpvfykr dvmltrlvvd klridfvgad ldytvyyags sdgrvhkvvq widnngeshs
      421 illdvfdvtp gepiqameis kehkalyvas dhrikqidlv mctrrydncl rcvhdpycgw
      481 dkdtntckpy epgllqdvsn ntadvcdssv gkrklvvtwg qsvhlgcfvk mpevlanqev
      541 rwyhyskekg ryqiaykyga ggdkfietse kglvivgvne qdagrydcwl ggsllcsyni
      601 tvdahrcsap aksndyqkiy sdwchefeky ksamkswerk qaqcasrqnd snqnlhanev
      661 ygtplv
//
LOCUS       XP_034193347            1287 aa            linear   INV 03-SEP-2025
DEFINITION  leucine rich repeat containing G protein-coupled receptor chaoptin
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193347
VERSION     XP_034193347.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337456.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193347.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1287
                     /product="leucine rich repeat containing G protein-coupled
                     receptor chaoptin isoform X1"
                     /calculated_mol_wt=148342
     Region          78..>415
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          79..98
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          99..123
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          124..147
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          148..172
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          173..196
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          197..221
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          222..245
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          246..274
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          275..298
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          299..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          322..342
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          370..764
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          371..396
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          470..494
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          495..521
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          522..545
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          546..569
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          572..595
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          596..619
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          620..643
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          667..1082
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          672..703
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          704..728
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          729..752
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          753..776
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          777..800
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          801..823
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          824..849
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          850..874
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          875..898
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          899..946
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          947..970
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          994..1038
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          1019..1042
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          1185..>1216
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     CDS             1..1287
                     /gene="chp"
                     /coded_by="XM_034337456.2:96..3959"
                     /db_xref="GeneID:117610266"
ORIGIN      
        1 mnlmtmvkvg ytlmiltlll mlwaslarmh elpvqyppcf fnplctcska ipdlgivtcy
       61 nvpmpripqp insskvfmlq lennglmflq pqflmntgly slrikhnpla dipdeaflgl
      121 erslwelelp ynrlervpsr sfrhlqklql ldltgnkisk ivpenwrgle nslqklrlgr
      181 naidklpada fagltyldml dlrennlkei dpsvfrdgma hlvhlylngn qlthvpyaql
      241 sslkrmkvld lsynriskml nhqqepeirg lqmsldilrl dfnqieslmp gdfqhfykin
      301 rtyldgnplt iieegafrds rirelyfcdc hlmevsspdf aglesslell dlsgnnittl
      361 pspvfqeydf lrtlifrenk iqtfspaevf ngfqyslynl dlsgrensvv slqdlrqmrn
      421 mrflsisrmp qstlstedfm eygmdikelr ivqsnlntik ghafmhvrgi kyldfsensi
      481 ssieneafse vghslltlri ahglsssise iphapfkflp nlqhldfsnn kikslpdtsf
      541 hflkrikrie lqdneiddir kgtfqgdihs yledvnfsfn kiktiqthtf vdlpkitmin
      601 lednaidkie rrafmnmkll qyvnlrgnki kditdeafqn lpdlefldls yndmnefdfa
      661 sfdqvgtlss fkvnashnei pklwinsttf tppttiggai qsnikvldls ynnisdimry
      721 yfkpveyslt hlyishnqls nvtqgvfgnm phlqwldlsh ndlievdfdc frntknlqvl
      781 flswnnimdi paealrplkk lrivdlshnk lrtlsdnmfg daniesldls hnqfmrlpmk
      841 tmsisaaasl sildmswnsl sgihttdaif rlrtltwldl synrlvrldd gvfsdlqylt
      901 hldlshnkql lletrgrtfh gledsllyld lsnisllsvp elplrrlqtl ylahnelasi
      961 paemasnlts lhyldlsand ltvvplitht lpelktfnia dnpitavsnt sflgiadsle
     1021 eldirrlsls tfesgafcka tklrrlyita ytgiknfnfp nlleynhglr hllidiqnet
     1081 nlgkemrgkf pnklfnitlt gqalkylnpe ilkgirnphl hfglyntsvn mvsrlifdna
     1141 dwvrnvtihv hnsdirtlyn plngykpgvp gkrfltkltv agsyfncdcd igwmenwqrk
     1201 hrqyqedrct tysefknfdr deeddqfdcw dngwdddlre sfclnknnis vlealktdle
     1261 cgwgtanyvr tsyhlvllfv vltttiy
//
LOCUS       XP_034193367            1275 aa            linear   INV 03-SEP-2025
DEFINITION  leucine rich repeat containing G protein-coupled receptor chaoptin
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193367
VERSION     XP_034193367.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337476.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193367.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1275
                     /product="leucine rich repeat containing G protein-coupled
                     receptor chaoptin isoform X2"
                     /calculated_mol_wt=146966
     Region          66..>403
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          67..86
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          87..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..160
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          161..184
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          185..209
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          210..233
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          234..262
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          263..286
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          287..309
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          310..330
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          358..752
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          359..384
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          458..482
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          483..509
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          510..533
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          534..557
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          560..583
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          584..607
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          608..631
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          655..1070
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          660..691
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          692..716
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          717..740
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          741..764
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          765..788
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          789..811
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          812..837
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          838..862
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          863..886
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          887..934
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          935..958
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          982..1026
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          1007..1030
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          1173..>1204
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     CDS             1..1275
                     /gene="chp"
                     /coded_by="XM_034337476.2:1..3828"
                     /db_xref="GeneID:117610266"
ORIGIN      
        1 mlkivitefd ddgearmhel pvqyppcffn plctcskaip dlgivtcynv pmpripqpin
       61 sskvfmlqle nnglmflqpq flmntglysl rikhnpladi pdeaflgler slwelelpyn
      121 rlervpsrsf rhlqklqlld ltgnkiskiv penwrglens lqklrlgrna idklpadafa
      181 gltyldmldl rennlkeidp svfrdgmahl vhlylngnql thvpyaqlss lkrmkvldls
      241 ynriskmlnh qqepeirglq msldilrldf nqieslmpgd fqhfykinrt yldgnpltii
      301 eegafrdsri relyfcdchl mevsspdfag lesslelldl sgnnittlps pvfqeydflr
      361 tlifrenkiq tfspaevfng fqyslynldl sgrensvvsl qdlrqmrnmr flsisrmpqs
      421 tlstedfmey gmdikelriv qsnlntikgh afmhvrgiky ldfsensiss ieneafsevg
      481 hslltlriah glsssiseip hapfkflpnl qhldfsnnki kslpdtsfhf lkrikrielq
      541 dneiddirkg tfqgdihsyl edvnfsfnki ktiqthtfvd lpkitminle dnaidkierr
      601 afmnmkllqy vnlrgnkikd itdeafqnlp dlefldlsyn dmnefdfasf dqvgtlssfk
      661 vnashneipk lwinsttftp pttiggaiqs nikvldlsyn nisdimryyf kpveyslthl
      721 yishnqlsnv tqgvfgnmph lqwldlshnd lievdfdcfr ntknlqvlfl swnnimdipa
      781 ealrplkklr ivdlshnklr tlsdnmfgda niesldlshn qfmrlpmktm sisaaaslsi
      841 ldmswnslsg ihttdaifrl rtltwldlsy nrlvrlddgv fsdlqylthl dlshnkqlll
      901 etrgrtfhgl edsllyldls nisllsvpel plrrlqtlyl ahnelasipa emasnltslh
      961 yldlsandlt vvplithtlp elktfniadn pitavsntsf lgiadsleel dirrlslstf
     1021 esgafckatk lrrlyitayt giknfnfpnl leynhglrhl lidiqnetnl gkemrgkfpn
     1081 klfnitltgq alkylnpeil kgirnphlhf glyntsvnmv srlifdnadw vrnvtihvhn
     1141 sdirtlynpl ngykpgvpgk rfltkltvag syfncdcdig wmenwqrkhr qyqedrctty
     1201 sefknfdrde eddqfdcwdn gwdddlresf clnknnisvl ealktdlecg wgtanyvrts
     1261 yhlvllfvvl tttiy
//
LOCUS       XP_034193376            1259 aa            linear   INV 03-SEP-2025
DEFINITION  leucine rich repeat containing G protein-coupled receptor chaoptin
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193376
VERSION     XP_034193376.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193376.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1259
                     /product="leucine rich repeat containing G protein-coupled
                     receptor chaoptin isoform X3"
                     /calculated_mol_wt=145131
     Region          50..>387
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          51..70
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          71..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          120..144
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          169..193
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          194..217
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          218..246
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          247..270
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          271..293
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          294..314
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          342..736
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          343..368
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          442..466
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          467..493
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          494..517
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          518..541
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          544..567
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          568..591
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          592..615
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          639..1054
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          644..675
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          676..700
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          701..724
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          725..748
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          749..772
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          773..795
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          796..821
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          822..846
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          847..870
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          871..918
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          919..942
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          966..1010
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          991..1014
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          1157..>1188
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     CDS             1..1259
                     /gene="chp"
                     /coded_by="XM_034337485.2:106..3885"
                     /db_xref="GeneID:117610266"
ORIGIN      
        1 mhelpvqypp cffnplctcs kaipdlgivt cynvpmprip qpinsskvfm lqlennglmf
       61 lqpqflmntg lyslrikhnp ladipdeafl glerslwele lpynrlervp srsfrhlqkl
      121 qlldltgnki skivpenwrg lenslqklrl grnaidklpa dafagltyld mldlrennlk
      181 eidpsvfrdg mahlvhlyln gnqlthvpya qlsslkrmkv ldlsynrisk mlnhqqepei
      241 rglqmsldil rldfnqiesl mpgdfqhfyk inrtyldgnp ltiieegafr dsrirelyfc
      301 dchlmevssp dfaglessle lldlsgnnit tlpspvfqey dflrtlifre nkiqtfspae
      361 vfngfqysly nldlsgrens vvslqdlrqm rnmrflsisr mpqstlsted fmeygmdike
      421 lrivqsnlnt ikghafmhvr gikyldfsen sissieneaf sevghslltl riahglsssi
      481 seiphapfkf lpnlqhldfs nnkikslpdt sfhflkrikr ielqdneidd irkgtfqgdi
      541 hsyledvnfs fnkiktiqth tfvdlpkitm inlednaidk ierrafmnmk llqyvnlrgn
      601 kikditdeaf qnlpdlefld lsyndmnefd fasfdqvgtl ssfkvnashn eipklwinst
      661 tftppttigg aiqsnikvld lsynnisdim ryyfkpveys lthlyishnq lsnvtqgvfg
      721 nmphlqwldl shndlievdf dcfrntknlq vlflswnnim dipaealrpl kklrivdlsh
      781 nklrtlsdnm fgdaniesld lshnqfmrlp mktmsisaaa slsildmswn slsgihttda
      841 ifrlrtltwl dlsynrlvrl ddgvfsdlqy lthldlshnk qllletrgrt fhgledslly
      901 ldlsnislls vpelplrrlq tlylahnela sipaemasnl tslhyldlsa ndltvvplit
      961 htlpelktfn iadnpitavs ntsflgiads leeldirrls lstfesgafc katklrrlyi
     1021 taytgiknfn fpnlleynhg lrhllidiqn etnlgkemrg kfpnklfnit ltgqalkyln
     1081 peilkgirnp hlhfglynts vnmvsrlifd nadwvrnvti hvhnsdirtl ynplngykpg
     1141 vpgkrfltkl tvagsyfncd cdigwmenwq rkhrqyqedr cttysefknf drdeeddqfd
     1201 cwdngwdddl resfclnknn isvlealktd lecgwgtany vrtsyhlvll fvvltttiy
//
LOCUS       XP_034193389             865 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin protein ligase E3A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193389
VERSION     XP_034193389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..865
                     /product="ubiquitin protein ligase E3A isoform X1"
                     /calculated_mol_wt=97099
     Region          31..85
                     /region_name="AZUL"
                     /note="Amino-terminal Zinc-binding domain of ubiquitin
                     ligase E3A; pfam16558"
                     /db_xref="CDD:465173"
     Region          512..863
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(518,551,562,619,798,827..828,831..835,855,862)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(647,650..651,653..654,657,666,668,672..673,675,681,
                     686,703,707)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..865
                     /gene="Ube3a"
                     /coded_by="XM_034337498.2:577..3174"
                     /db_xref="GeneID:117610280"
ORIGIN      
        1 msakgqgdpg edhasrvgae spcsdmkrav akklieryfy qltdgcgnpd cdnqncassg
       61 kvtnltpnqa aaqaiqlfsq earlcdgtqp skvarttlep gpaslamslp vknvnptcsd
      121 eagkqdpylt eaklleiier cksegsysll irtlgevfsc akalslsfqk tvkndsplaa
      181 lldrasdtll rppgdmdkea irsiqgedke edssepsptv pnnddtsvdl pavrrafaal
      241 wslpgeafks alvnalvtla dnieldlrvf rimpwdnmds llnvflivfe ipmlgnseyl
      301 elafhmlcka lscfpivaqa klarvwakhc ksrlpsilqa lqelitvkvi ggsftrdycv
      361 qdadtitapt kvmkilyyas mlsgeldepd lnmdedvest sndksvrsss sqipqdplak
      421 elgitvldar kpfipftdfy neplsdaiem dkdfayykse epmkfsfmny afiltpatkt
      481 lglyydnrir myserrmsfl qtivghptnp ylrlkvrrdr liddalvelq miavehpldl
      541 kkqlvvefeg eqgvdeggvs keffqlivee ifnpdygmfi tqedtqmtwf nptsfesdah
      601 ftligvvlgl aiynnvildv hfsmvvyrkl lgrkgcfadl edwsptlyrt lkelmdytgd
      661 dmsetfmqtf kvaykdvfgs itfhdlkeng deifvtqenk kefvdhyadf llnksverqf
      721 kafrcgfqmv tdesplallf rpeeieqlvc gskvfdfael eaateyeggy tvdseavrnf
      781 wrvahslppe sqrrllqftt gsdrvpvggl srlkmviarh gpdsdrlpia htcynvlllp
      841 dystieklqd rllkainysk gfgml
//
LOCUS       XP_034193398             864 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin protein ligase E3A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193398
VERSION     XP_034193398.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337507.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..864
                     /product="ubiquitin protein ligase E3A isoform X2"
                     /calculated_mol_wt=97028
     Region          31..85
                     /region_name="AZUL"
                     /note="Amino-terminal Zinc-binding domain of ubiquitin
                     ligase E3A; pfam16558"
                     /db_xref="CDD:465173"
     Region          511..862
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(517,550,561,618,797,826..827,830..834,854,861)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(646,649..650,652..653,656,665,667,671..672,674,680,
                     685,702,706)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..864
                     /gene="Ube3a"
                     /coded_by="XM_034337507.2:578..3172"
                     /db_xref="GeneID:117610280"
ORIGIN      
        1 msakgqgdpg edhasrvgae spcsdmkrav akklieryfy qltdgcgnpd cdnqncassg
       61 kvtnltpnqa aaqaiqlfsq earlcdgtqp skvarttlep gpaslamslp vknvnptcsd
      121 egkqdpylte aklleiierc ksegsyslli rtlgevfsca kalslsfqkt vkndsplaal
      181 ldrasdtllr ppgdmdkeai rsiqgedkee dssepsptvp nnddtsvdlp avrrafaalw
      241 slpgeafksa lvnalvtlad nieldlrvfr impwdnmdsl lnvflivfei pmlgnseyle
      301 lafhmlckal scfpivaqak larvwakhck srlpsilqal qelitvkvig gsftrdycvq
      361 dadtitaptk vmkilyyasm lsgeldepdl nmdedvests ndksvrssss qipqdplake
      421 lgitvldark pfipftdfyn eplsdaiemd kdfayyksee pmkfsfmnya filtpatktl
      481 glyydnrirm yserrmsflq tivghptnpy lrlkvrrdrl iddalvelqm iavehpldlk
      541 kqlvvefege qgvdeggvsk effqliveei fnpdygmfit qedtqmtwfn ptsfesdahf
      601 tligvvlgla iynnvildvh fsmvvyrkll grkgcfadle dwsptlyrtl kelmdytgdd
      661 msetfmqtfk vaykdvfgsi tfhdlkengd eifvtqenkk efvdhyadfl lnksverqfk
      721 afrcgfqmvt desplallfr peeieqlvcg skvfdfaele aateyeggyt vdseavrnfw
      781 rvahslppes qrrllqfttg sdrvpvggls rlkmviarhg pdsdrlpiah tcynvlllpd
      841 ystieklqdr llkainyskg fgml
//
LOCUS       XP_034193404             840 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin protein ligase E3A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193404
VERSION     XP_034193404.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337513.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..840
                     /product="ubiquitin protein ligase E3A isoform X3"
                     /calculated_mol_wt=94629
     Region          6..60
                     /region_name="AZUL"
                     /note="Amino-terminal Zinc-binding domain of ubiquitin
                     ligase E3A; pfam16558"
                     /db_xref="CDD:465173"
     Region          487..838
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(493,526,537,594,773,802..803,806..810,830,837)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(622,625..626,628..629,632,641,643,647..648,650,656,
                     661,678,682)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..840
                     /gene="Ube3a"
                     /coded_by="XM_034337513.2:393..2915"
                     /db_xref="GeneID:117610280"
ORIGIN      
        1 mkravakkli eryfyqltdg cgnpdcdnqn cassgkvtnl tpnqaaaqai qlfsqearlc
       61 dgtqpskvar ttlepgpasl amslpvknvn ptcsdeagkq dpylteakll eiierckseg
      121 sysllirtlg evfscakals lsfqktvknd splaalldra sdtllrppgd mdkeairsiq
      181 gedkeedsse psptvpnndd tsvdlpavrr afaalwslpg eafksalvna lvtladniel
      241 dlrvfrimpw dnmdsllnvf livfeipmlg nseylelafh mlckalscfp ivaqaklarv
      301 wakhcksrlp silqalqeli tvkviggsft rdycvqdadt itaptkvmki lyyasmlsge
      361 ldepdlnmde dvestsndks vrssssqipq dplakelgit vldarkpfip ftdfynepls
      421 daiemdkdfa yykseepmkf sfmnyafilt patktlglyy dnrirmyser rmsflqtivg
      481 hptnpylrlk vrrdrlidda lvelqmiave hpldlkkqlv vefegeqgvd eggvskeffq
      541 liveeifnpd ygmfitqedt qmtwfnptsf esdahftlig vvlglaiynn vildvhfsmv
      601 vyrkllgrkg cfadledwsp tlyrtlkelm dytgddmset fmqtfkvayk dvfgsitfhd
      661 lkengdeifv tqenkkefvd hyadfllnks verqfkafrc gfqmvtdesp lallfrpeei
      721 eqlvcgskvf dfaeleaate yeggytvdse avrnfwrvah slppesqrrl lqfttgsdrv
      781 pvgglsrlkm viarhgpdsd rlpiahtcyn vlllpdysti eklqdrllka inyskgfgml
//
LOCUS       XP_034193414             840 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin protein ligase E3A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193414
VERSION     XP_034193414.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337523.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..840
                     /product="ubiquitin protein ligase E3A isoform X3"
                     /calculated_mol_wt=94629
     Region          6..60
                     /region_name="AZUL"
                     /note="Amino-terminal Zinc-binding domain of ubiquitin
                     ligase E3A; pfam16558"
                     /db_xref="CDD:465173"
     Region          487..838
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(493,526,537,594,773,802..803,806..810,830,837)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(622,625..626,628..629,632,641,643,647..648,650,656,
                     661,678,682)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..840
                     /gene="Ube3a"
                     /coded_by="XM_034337523.2:831..3353"
                     /db_xref="GeneID:117610280"
ORIGIN      
        1 mkravakkli eryfyqltdg cgnpdcdnqn cassgkvtnl tpnqaaaqai qlfsqearlc
       61 dgtqpskvar ttlepgpasl amslpvknvn ptcsdeagkq dpylteakll eiierckseg
      121 sysllirtlg evfscakals lsfqktvknd splaalldra sdtllrppgd mdkeairsiq
      181 gedkeedsse psptvpnndd tsvdlpavrr afaalwslpg eafksalvna lvtladniel
      241 dlrvfrimpw dnmdsllnvf livfeipmlg nseylelafh mlckalscfp ivaqaklarv
      301 wakhcksrlp silqalqeli tvkviggsft rdycvqdadt itaptkvmki lyyasmlsge
      361 ldepdlnmde dvestsndks vrssssqipq dplakelgit vldarkpfip ftdfynepls
      421 daiemdkdfa yykseepmkf sfmnyafilt patktlglyy dnrirmyser rmsflqtivg
      481 hptnpylrlk vrrdrlidda lvelqmiave hpldlkkqlv vefegeqgvd eggvskeffq
      541 liveeifnpd ygmfitqedt qmtwfnptsf esdahftlig vvlglaiynn vildvhfsmv
      601 vyrkllgrkg cfadledwsp tlyrtlkelm dytgddmset fmqtfkvayk dvfgsitfhd
      661 lkengdeifv tqenkkefvd hyadfllnks verqfkafrc gfqmvtdesp lallfrpeei
      721 eqlvcgskvf dfaeleaate yeggytvdse avrnfwrvah slppesqrrl lqfttgsdrv
      781 pvgglsrlkm viarhgpdsd rlpiahtcyn vlllpdysti eklqdrllka inyskgfgml
//
LOCUS       XP_034193423             840 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin protein ligase E3A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193423
VERSION     XP_034193423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..840
                     /product="ubiquitin protein ligase E3A isoform X3"
                     /calculated_mol_wt=94629
     Region          6..60
                     /region_name="AZUL"
                     /note="Amino-terminal Zinc-binding domain of ubiquitin
                     ligase E3A; pfam16558"
                     /db_xref="CDD:465173"
     Region          487..838
                     /region_name="HECTc"
                     /note="HECT domain; C-terminal catalytic domain of a
                     subclass of Ubiquitin-protein ligase (E3). It binds
                     specific ubiquitin-conjugating enzymes (E2), accepts
                     ubiquitin from E2, transfers ubiquitin to substrate lysine
                     side chains, and transfers additional...; cd00078"
                     /db_xref="CDD:238033"
     Site            order(493,526,537,594,773,802..803,806..810,830,837)
                     /site_type="active"
                     /note="catalytic cleft [active]"
                     /db_xref="CDD:238033"
     Site            order(622,625..626,628..629,632,641,643,647..648,650,656,
                     661,678,682)
                     /site_type="active"
                     /note="E2 interaction site [active]"
                     /db_xref="CDD:238033"
     CDS             1..840
                     /gene="Ube3a"
                     /coded_by="XM_034337532.2:692..3214"
                     /db_xref="GeneID:117610280"
ORIGIN      
        1 mkravakkli eryfyqltdg cgnpdcdnqn cassgkvtnl tpnqaaaqai qlfsqearlc
       61 dgtqpskvar ttlepgpasl amslpvknvn ptcsdeagkq dpylteakll eiierckseg
      121 sysllirtlg evfscakals lsfqktvknd splaalldra sdtllrppgd mdkeairsiq
      181 gedkeedsse psptvpnndd tsvdlpavrr afaalwslpg eafksalvna lvtladniel
      241 dlrvfrimpw dnmdsllnvf livfeipmlg nseylelafh mlckalscfp ivaqaklarv
      301 wakhcksrlp silqalqeli tvkviggsft rdycvqdadt itaptkvmki lyyasmlsge
      361 ldepdlnmde dvestsndks vrssssqipq dplakelgit vldarkpfip ftdfynepls
      421 daiemdkdfa yykseepmkf sfmnyafilt patktlglyy dnrirmyser rmsflqtivg
      481 hptnpylrlk vrrdrlidda lvelqmiave hpldlkkqlv vefegeqgvd eggvskeffq
      541 liveeifnpd ygmfitqedt qmtwfnptsf esdahftlig vvlglaiynn vildvhfsmv
      601 vyrkllgrkg cfadledwsp tlyrtlkelm dytgddmset fmqtfkvayk dvfgsitfhd
      661 lkengdeifv tqenkkefvd hyadfllnks verqfkafrc gfqmvtdesp lallfrpeei
      721 eqlvcgskvf dfaeleaate yeggytvdse avrnfwrvah slppesqrrl lqfttgsdrv
      781 pvgglsrlkm viarhgpdsd rlpiahtcyn vlllpdysti eklqdrllka inyskgfgml
//
LOCUS       XP_034193435             309 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610302 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193435
VERSION     XP_034193435.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337544.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..309
                     /product="uncharacterized protein LOC117610302 isoform X1"
                     /calculated_mol_wt=35470
     CDS             1..309
                     /gene="LOC117610302"
                     /coded_by="XM_034337544.2:812..1741"
                     /db_xref="GeneID:117610302"
ORIGIN      
        1 mctdcgsaat srvscftnmn diqnqnnfdf vpksynekwh ldnkclshnq flegdtckfg
       61 kssgksltsc dfckclddkl kitgenslnq nssglckhla eiynettden isnqksgdwn
      121 reeeiiehhs epviekpsih nwkwknpysf snfnrrsrnr fiqekkfstt strdgcnkvd
      181 rkgtnflhnf reylvnyqes cgvprteysf saliqimgqa tgrsllally vmlnilplae
      241 illyvlrfvl dkvinigssr dfrqtilrcf vfttellsiy iclififgfi vlpilqtvig
      301 ivakimlyn
//
LOCUS       XP_034193441             273 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610302 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193441
VERSION     XP_034193441.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337550.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..273
                     /product="uncharacterized protein LOC117610302 isoform X2"
                     /calculated_mol_wt=31315
     CDS             1..273
                     /gene="LOC117610302"
                     /coded_by="XM_034337550.2:1130..1951"
                     /db_xref="GeneID:117610302"
ORIGIN      
        1 mklinkpliv nysaflegdt ckfgkssgks ltscdfckcl ddklkitgen slnqnssglc
       61 khlaeiynet tdenisnqks gdwnreeeii ehhsepviek psihnwkwkn pysfsnfnrr
      121 srnrfiqekk fsttstrdgc nkvdrkgtnf lhnfreylvn yqescgvprt eysfsaliqi
      181 mgqatgrsll allyvmlnil plaeillyvl rfvldkvini gssrdfrqti lrcfvfttel
      241 lsiyiclifi fgfivlpilq tvigivakim lyn
//
LOCUS       XP_034195976             856 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611793 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195976
VERSION     XP_034195976.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340085.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..856
                     /product="uncharacterized protein LOC117611793 isoform X1"
                     /calculated_mol_wt=97191
     Region          498..518
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(505..507,509..510,512,517,534,537..541,543..544)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          529..551
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..856
                     /gene="LOC117611793"
                     /coded_by="XM_034340085.2:216..2786"
                     /db_xref="GeneID:117611793"
ORIGIN      
        1 msysynkcss smssrgrgfn srggssyrgr gggggnewns gstggnmssr ggysssrggr
       61 fkysttgyds rskynsggse ryssrggrge hsnsykrpre sysgrddhrs ssdstrkrmr
      121 sdsyqgggsg sgsqrysssy ggssasaayd dnkgssssav yedkrqsera ssyhrsedrh
      181 sasrsyappp pprisemapp tqrytssrgr ishqssnyrg ristrgsgrg ggfhrmssrs
      241 dvimarkrtl hsldyrrkll gsrsrdyiqr vrmtttklrr ssgttrlsgs kkesgsgtsm
      301 kdkdremtka inaefsddde edddnksnwd ddekpherdd eeedeeeekk kkdekrkkek
      361 qdrerqntdd eeekedeike eeddrkhedd eddgddeerd dendktdhgr tgseelpsrr
      421 dgrkfikltc phcahrsvtf keyslhlysg rhsaamrria srhkatltrm rvlqrqdqrr
      481 veardaargt lpsrtmfcpi cklnyrslka vhqlsdshrq mkrfltpfcr tcrmqfrspm
      541 lfethmcsle hikrksilke rmnagngeae adssgpeedd kevnlenfmt ldsvgdvdae
      601 deespekkke kpesegpndt ekkpkskqmi kvgaeyikrv evqfcelckm ylprsenser
      661 aialhcstrs hlkryvrdnd dkalrrqaer ihlqsssstn tttpnavnst enaksptnse
      721 plsanntlpt tttdgtdate sgkpieqkgn vepeknikdn kngqaeeddd ddypadssdk
      781 lwddvdkdlg dilreteagg kssddedsry drfrnsdkkq phsgkdkdrd nekneideke
      841 sikkqelkvk iekidm
//
LOCUS       XP_034195977             855 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611793 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195977
VERSION     XP_034195977.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340086.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..855
                     /product="uncharacterized protein LOC117611793 isoform X2"
                     /calculated_mol_wt=97120
     Region          498..518
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(505..507,509..510,512,517,534,537..541,543..544)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          529..551
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..855
                     /gene="LOC117611793"
                     /coded_by="XM_034340086.2:215..2782"
                     /db_xref="GeneID:117611793"
ORIGIN      
        1 msysynkcss smssrgrgfn srggssyrgr gggggnewns gstggnmssr ggysssrggr
       61 fkysttgyds rskynsggse ryssrggrge hsnsykrpre sysgrddhrs ssdstrkrmr
      121 sdsyqgggsg sgsqrysssy ggssasaayd dnkgssssav yedkrqsera ssyhrsedrh
      181 sasrsyappp pprisemapp tqrytssrgr ishqssnyrg ristrgsgrg ggfhrmssrs
      241 dvimarkrtl hsldyrrkll gsrsrdyiqr vrmtttklrr ssgttrlsgs kkesgsgtsm
      301 kdkdremtka inaefsddde edddnksnwd ddekpherdd eeedeeeekk kkdekrkkek
      361 qdrerqntdd eeekedeike eeddrkhedd eddgddeerd dendktdhgr tgseelpsrr
      421 dgrkfikltc phcahrsvtf keyslhlysg rhsaamrria srhkatltrm rvlqrqdqrr
      481 veardaargt lpsrtmfcpi cklnyrslka vhqlsdshrq mkrfltpfcr tcrmqfrspm
      541 lfethmcsle hikrksilke rmnagngeae adssgpeedd kevnlenfmt ldsvgdvded
      601 eespekkkek pesegpndte kkpkskqmik vgaeyikrve vqfcelckmy lprsensera
      661 ialhcstrsh lkryvrdndd kalrrqaeri hlqsssstnt ttpnavnste naksptnsep
      721 lsanntlptt ttdgtdates gkpieqkgnv epeknikdnk ngqaeedddd dypadssdkl
      781 wddvdkdlgd ilreteaggk ssddedsryd rfrnsdkkqp hsgkdkdrdn ekneidekes
      841 ikkqelkvki ekidm
//
LOCUS       XP_034195978             855 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611793 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195978
VERSION     XP_034195978.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340087.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..855
                     /product="uncharacterized protein LOC117611793 isoform X3"
                     /calculated_mol_wt=97104
     Region          497..517
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(504..506,508..509,511,516,533,536..540,542..543)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          528..550
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..855
                     /gene="LOC117611793"
                     /coded_by="XM_034340087.2:216..2783"
                     /db_xref="GeneID:117611793"
ORIGIN      
        1 msysynkcss smssrgrgfn srggssyrgr gggggnewns gstggnmssr ggysssrggr
       61 fkysttgyds rskynsggse ryssrggrge hsnsykrpre sysgrddhrs ssdstrkrmr
      121 sdsyqgggsg sgsqrysssy ggssasaayd dnkgssssav yedkrqsera ssyhrsedrh
      181 sasrsyappp pprisemapp tqrytssrgr ishqssnyrg ristrgsgrg ggfhrmssrs
      241 dvimarkrtl hsldyrrkll gsrsrdyiqr vrmtttklrr sgttrlsgsk kesgsgtsmk
      301 dkdremtkai naefsdddee dddnksnwdd dekpherdde eedeeeekkk kdekrkkekq
      361 drerqntdde eekedeikee eddrkhedde ddgddeerdd endktdhgrt gseelpsrrd
      421 grkfikltcp hcahrsvtfk eyslhlysgr hsaamrrias rhkatltrmr vlqrqdqrrv
      481 eardaargtl psrtmfcpic klnyrslkav hqlsdshrqm krfltpfcrt crmqfrspml
      541 fethmcsleh ikrksilker mnagngeaea dssgpeeddk evnlenfmtl dsvgdvdaed
      601 eespekkkek pesegpndte kkpkskqmik vgaeyikrve vqfcelckmy lprsensera
      661 ialhcstrsh lkryvrdndd kalrrqaeri hlqsssstnt ttpnavnste naksptnsep
      721 lsanntlptt ttdgtdates gkpieqkgnv epeknikdnk ngqaeedddd dypadssdkl
      781 wddvdkdlgd ilreteaggk ssddedsryd rfrnsdkkqp hsgkdkdrdn ekneidekes
      841 ikkqelkvki ekidm
//
LOCUS       XP_034195979             811 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611793 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195979
VERSION     XP_034195979.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340088.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..811
                     /product="uncharacterized protein LOC117611793 isoform X4"
                     /calculated_mol_wt=91928
     Region          498..518
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     Site            order(505..507,509..510,512,517,534,537..541,543..544)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275371"
     Region          529..551
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275371"
     CDS             1..811
                     /gene="LOC117611793"
                     /coded_by="XM_034340088.2:216..2651"
                     /db_xref="GeneID:117611793"
ORIGIN      
        1 msysynkcss smssrgrgfn srggssyrgr gggggnewns gstggnmssr ggysssrggr
       61 fkysttgyds rskynsggse ryssrggrge hsnsykrpre sysgrddhrs ssdstrkrmr
      121 sdsyqgggsg sgsqrysssy ggssasaayd dnkgssssav yedkrqsera ssyhrsedrh
      181 sasrsyappp pprisemapp tqrytssrgr ishqssnyrg ristrgsgrg ggfhrmssrs
      241 dvimarkrtl hsldyrrkll gsrsrdyiqr vrmtttklrr ssgttrlsgs kkesgsgtsm
      301 kdkdremtka inaefsddde edddnksnwd ddekpherdd eeedeeeekk kkdekrkkek
      361 qdrerqntdd eeekedeike eeddrkhedd eddgddeerd dendktdhgr tgseelpsrr
      421 dgrkfikltc phcahrsvtf keyslhlysg rhsaamrria srhkatltrm rvlqrqdqrr
      481 veardaargt lpsrtmfcpi cklnyrslka vhqlsdshrq mkrfltpfcr tcrmqfrspm
      541 lfethmcsle hikrksilke rmnagngeae adssgpeedd kevnlenfmt ldsvgdvdae
      601 deespekkke kpesegpndt ekkpkskqmi kvgaeyikrv evqfcelckm ylprsenser
      661 aialhcstrs hlkryvrdnd dkalrrqaer ihlqsssstn tttpnavnst enaksptnse
      721 plsanntlpt tttdgtdate sgkpieqkgn vepeknikdn kngqaeeddd ddypadssdk
      781 lwddvdkdlg dilreteagv rlktneylyl h
//
LOCUS       XP_034195980             692 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611794 [Osmia lignaria lignaria].
ACCESSION   XP_034195980
VERSION     XP_034195980.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340089.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195980.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..692
                     /product="uncharacterized protein LOC117611794"
                     /calculated_mol_wt=76742
     Region          <178..490
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <584..654
                     /region_name="GvpP"
                     /note="Gas vesicle protein YhaH [General function
                     prediction only]; COG4980"
                     /db_xref="CDD:444004"
     CDS             1..692
                     /gene="LOC117611794"
                     /coded_by="XM_034340089.2:92..2170"
                     /db_xref="GeneID:117611794"
ORIGIN      
        1 mfkllqifai lgiaavlalp ltsnedesnt ttakdsesss ttpddqntvt pevpnhsgsp
       61 dngnqdnden nnntssadik nktsnviddl eqlvqsvaha ienelknaan stlnllenak
      121 gkvnatkdki knavqnatrt legilwnikn qtdnnlerlr natqnnvnki kdelsnvvqe
      181 ardgiatlkn dtseighdis erlenllnks vdalknvsqs vnnaidqakq daaevfeqak
      241 natseklqel kdkgsellqn vtddlkslrq egedilskie dslndlknet slfvhsiane
      301 vqekineikq nvykavneta qlvnstlanl seeaqqigee iknqndhikd nitdimqhlk
      361 eklpdiankt mdtlqqvveg fvnefeqikg nvsnivykvv kdvlseilri kqeisngign
      421 mknkienien egkdvlhnat dkiqddikkv tdiistlvgd vkdqtqrrlq elkdkvdemr
      481 heseamkdni khqveqvhkn isdalkklen dmnntlrnat nkaleiinnv knstqgmike
      541 vmqntaravn eakdqfedak nktavalnqt lsslrqdagk isnqtketis nvvnelknqs
      601 snaldslkni tkkalndake ksrkfaedlk dsigkiaeds snlikdalrs vqnivrdvfg
      661 dinngientv ngikdiasgi fdevagrpkw sk
//
LOCUS       XP_076546523             394 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305718 [Osmia lignaria lignaria].
ACCESSION   XP_076546523
VERSION     XP_076546523.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690408.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..394
                     /product="uncharacterized protein LOC143305718"
                     /calculated_mol_wt=44514
     CDS             1..394
                     /gene="LOC143305718"
                     /coded_by="XM_076690408.1:4..1188"
                     /db_xref="GeneID:143305718"
ORIGIN      
        1 manitsimsm eqchdicped iansctnvtv snteinsngs ksledlqcfv cdakiqgrhy
       61 alatcrtqts rtkvieklge lvgerymvvi seddvicrsc anlintldrl eveiynvrdn
      121 vlrfleqkys leegellgnn ekqkrsqppq itkcnnqlit ncqgkrrdvi lssnvaektk
      181 hkksnvwlqc dkcqyttlhn sfmvhhvrnh ikqkvfcdkc gvqfsekehk shncslkehl
      241 nvkntvqnep qaesslkhit etildipile knmqqnvpim aietytgsqi sncnenipii
      301 rlssaenlpi qnilssddss gqpiyvrvlq pveinetsth stimasnsda dltmkfkdns
      361 dkqiltlted gnlemteiac wndiqssqsn iifq
//
LOCUS       XP_034193602             591 aa            linear   INV 03-SEP-2025
DEFINITION  dihydrolipoyllysine-residue acetyltransferase component of pyruvate
            dehydrogenase complex [Osmia lignaria lignaria].
ACCESSION   XP_034193602
VERSION     XP_034193602.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193602.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..591
                     /product="dihydrolipoyllysine-residue acetyltransferase
                     component of pyruvate dehydrogenase complex"
                     /calculated_mol_wt=63683
     Region          50..>178
                     /region_name="PRK11892"
                     /note="pyruvate dehydrogenase subunit beta; Provisional"
                     /db_xref="CDD:237011"
     Region          170..575
                     /region_name="PDHac_trf_mito"
                     /note="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase, long form; TIGR01349"
                     /db_xref="CDD:273567"
     CDS             1..591
                     /gene="LOC117610390"
                     /coded_by="XM_034337711.2:149..1924"
                     /db_xref="GeneID:117610390"
ORIGIN      
        1 mlnmaqtmip riallsikns nrvlpricvp lkyqrtcfht swvldvkgke llmpslsptm
       61 etgtiikwlk kegdkiepgd avadiqtdka vvtmevddes vlakilipeg tkdvkigtli
      121 altvepdedw ksvempdsas aatvtppapp agastpaatv taepppgqnn vampalsptm
      181 ttgtivkwlk kegdsiepgd alaeiqtdka vmtfefedea vlakllvpeg sqievgqlia
      241 itvekgmdwk nvvvpsttkp staaavpppt agdkkpassg qvyglavkrl leeyglnpds
      301 ikgtgrpnrl lksdilayiq annikkvapk vepppavetg kkeraaspsk khvptgrpsa
      361 yedipvsnir gviakrlgea kstiphsyay idikmdklme irselkadgi nvsvndfitk
      421 aaalalvecp fintlyknnq itqmprvdis vavstdngli tpivfdtaak slvdisknik
      481 elaekarngr lkpeefqggt ftisnlgmfg ikqfsaiinp pqtailavgs grkeldatlq
      541 kitkmsatls ydrraidedq aadflavlra mledptffva grlralrysq n
//
LOCUS       XP_034193617             502 aa            linear   INV 03-SEP-2025
DEFINITION  p21 (RAC1) activated kinase 3 [Osmia lignaria lignaria].
ACCESSION   XP_034193617
VERSION     XP_034193617.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337726.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="p21 (RAC1) activated kinase 3"
                     /calculated_mol_wt=56435
     Region          22..79
                     /region_name="PBD"
                     /note="P21-Rho-binding domain; pfam00786"
                     /db_xref="CDD:395634"
     Site            order(23,26,29,31,34,51,55)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238526"
     Region          221..478
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(233..236,239,241,254,256,285,301..304,346,350..351,
                     353,363..364)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..502
                     /gene="Pak3"
                     /coded_by="XM_034337726.2:1055..2563"
                     /db_xref="GeneID:117610393"
ORIGIN      
        1 mslsitklfs rkktgtvdtv aeigpptnvs hkfhvsknae tgqleglpes wirllntqit
       61 kseqdehpaa alqaikfyny sikrkpeeiv fkpfvtqdli eeesqeidki lskkyqsgds
      121 dgsisahstd sqvqlpelpp kvnktpkptp ricpdrtekt lteiledlnt yqiddeqlpe
      181 ddnsaniiee spilrrktey tgvklsdeev fdelraichn gdpnlrfekt kevgagasgt
      241 vfiatdlmvd qrvaikdidm skqpkkelil teikvlkefq hpnlvnflda ylvhdhlwvv
      301 melleggplt dvvtetvmkd aqiaavcrev lkaisflhtr giihrdiksd nvllgmngtv
      361 kvtdfgfcan idgdekrqtm vgtpywmape vvtrkqygkk vdiwslgima iemiegeppy
      421 mketplraly liaavgkpsi prwetlsptf qnflerclav evderatade llshpflenc
      481 aelttltpli raaqrilhka fn
//
LOCUS       XP_034193630             409 aa            linear   INV 03-SEP-2025
DEFINITION  branched chain amino acid transaminase [Osmia lignaria lignaria].
ACCESSION   XP_034193630
VERSION     XP_034193630.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337739.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193630.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="branched chain amino acid transaminase"
                     /calculated_mol_wt=45880
     Region          101..397
                     /region_name="BCAT_beta_family"
                     /note="Branched-chain aminotransferase catalyses the
                     transamination of the branched-chain amino acids leusine,
                     isoleucine and valine to their respective alpha-keto
                     acids, alpha-ketoisocaproate,
                     alpha-keto-beta-methylvalerate and alpha-ketoisovalerate.
                     The...; cd01557"
                     /db_xref="CDD:238798"
     Site            order(101..104,107..111,113,115,146,183,185,191..193,197,
                     204,245,250,252,256,270)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:238798"
     Site            order(110,115,139,195,243,248,278,311..312,355)
                     /site_type="active"
                     /note="substrate-cofactor binding pocket [active]"
                     /db_xref="CDD:238798"
     Site            243
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238798"
     CDS             1..409
                     /gene="Bcat"
                     /coded_by="XM_034337739.2:360..1589"
                     /db_xref="GeneID:117610399"
ORIGIN      
        1 mvqmrrqilt cggllnqlqp nvrrssslqi rdkkviqper sfkyadlsvr lagpeqlqpk
       61 pnvsaltfgk yftdhmlkif yhealggwqv peitpfenlv lhpaskvlhy avelfeglka
      121 yrgvdgkiri frpdlnmerm nstairtglp tfigdelikc csrlisidqe wvphsiassl
      181 yirptligid ptlgvasses allyvilspv gsyfsktdad vgisllanpq ytrawpggcg
      241 nykmgsnygp tiyvqreake kglqqvlwly gkdhevtevg tmnifmfcin ddgekelvtp
      301 plssglilpg itrksilaia rewnqfkvse rklcmdevcq llaenrllel fgagtagiic
      361 pvsyiefver sldiptlkqp dpvykmflkq lkdiqygvip dhpwalpie
//
LOCUS       XP_076546524             145 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305719 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546524
VERSION     XP_076546524.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690409.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..145
                     /product="uncharacterized protein LOC143305719 isoform X1"
                     /calculated_mol_wt=16751
     CDS             1..145
                     /gene="LOC143305719"
                     /coded_by="XM_076690409.1:4894..5331"
                     /db_xref="GeneID:143305719"
ORIGIN      
        1 mrqeilcfem qrpmhrivrs hgnkrgtigs rstsaviltk ysavrkrsrr epvdfgaill
       61 qysdakdrss ykhspdpfra rsmilqssyq rifrlysvvf heqknsslpi fkkrpglcrn
      121 gqcrlqgpld alhrismdta dsrrl
//
LOCUS       XP_076546525              80 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305719 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546525
VERSION     XP_076546525.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690410.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..80
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..80
                     /product="uncharacterized protein LOC143305719 isoform X2"
                     /calculated_mol_wt=9290
     CDS             1..80
                     /gene="LOC143305719"
                     /coded_by="XM_076690410.1:491..733"
                     /db_xref="GeneID:143305719"
ORIGIN      
        1 mryqdtkifs ceaetgnysk lecsrcfgvy rsskndqvyv emdnavskdh wtrcivlawt
       61 prirdgcdfl ngvkkkrekn
//
LOCUS       XP_034195415             751 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611560 [Osmia lignaria lignaria].
ACCESSION   XP_034195415
VERSION     XP_034195415.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339524.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195415.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..751
                     /product="uncharacterized protein LOC117611560"
                     /calculated_mol_wt=84567
     CDS             1..751
                     /gene="LOC117611560"
                     /coded_by="XM_034339524.2:46..2301"
                     /db_xref="GeneID:117611560"
ORIGIN      
        1 mnlsmkiier sietensgnv ddnkiylvsl vaydfrnfli mlrghfgsfe lvraifnpaf
       61 rrnlsgasir yaedkdkkka dnvfswlkdi frsddgmqea rkvsisggpv tvldypetne
      121 aslinlkfdq gsieyrrnfy dpknnnidye ksqrfsprhh llalsekidt sirtimgctp
      181 rntkppirep nmpekqekkd ekkekkkkkp virmegglgd tdqndptlka egtlhdnldi
      241 nlrhqwgvrl peevaksenm elqqsvgsgy qvpvydmknl qltmssskds esidaktegq
      301 merrettpln nakqlakkse ssknvspgmm teggvvdedy nippgthvda evtawkelvl
      361 rskvpvdpsk eqfipedgrq aemlsirpea prpkldvqav ptssssdetm neeedeaefs
      421 sddrtsfkpd iaragsgadv qkldskklht gstcftrkgs srildtidns vgfvasqral
      481 dfststdsgw ersdqstake tepvpiaaev qaesrfdqrs sdntelaenp sdnqgvnveg
      541 rqettanfdq qrqaemdqdf rdslnsiadr emdmemsmns ardedqsmre nsrmaidrqd
      601 qrqaenegql sslsedfqas fdyastpyed dfyptyesms leavpeksiv kkskeplqst
      661 aassdvqndq gyvnpademe yrdeeytsdg nyiripgdpy pyspehfnkw snnettkpge
      721 klaeiqpsvr klhndahvst lggaqgkevp n
//
LOCUS       XP_034195416             452 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611561 [Osmia lignaria lignaria].
ACCESSION   XP_034195416
VERSION     XP_034195416.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339525.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195416.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="uncharacterized protein LOC117611561"
                     /calculated_mol_wt=53280
     CDS             1..452
                     /gene="LOC117611561"
                     /coded_by="XM_034339525.2:508..1866"
                     /db_xref="GeneID:117611561"
ORIGIN      
        1 mrlilfelys llfllteida agigeawqvs pyakdywnhp swprsdssaf eirtvsgfgh
       61 lnpfenvrks snpeeiepna ipddaieeki irtgimmtvl qpnnkeileg tkkivsrmrr
      121 dadnetnlee istnknvrea rlsssdswst qplsiefprr ssldqmiqtv ddeelansrg
      181 yaipradfvt shhrrsyehr eprdmpvtrn yddydyswyr navrerdydp mtyrrgfsyy
      241 ypdryrmerd yytrlpndys yyydryrdee ldlygrsrpt pkpkriiyya tlpeivrkpv
      301 dlrnyprpyd ggtrtptsrd gsykripgnv dptryryrhp ydsydsyskr ssiadrpypy
      361 peeesrrkvt lenlrnneql dqnndrkigs qrndgklpwp vvqigtevsv kdderipgrk
      421 ifgesngyer yqsaqlqkap datgsselqs dn
//
LOCUS       XP_034195414             565 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611558 [Osmia lignaria lignaria].
ACCESSION   XP_034195414
VERSION     XP_034195414.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339523.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195414.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..565
                     /product="uncharacterized protein LOC117611558"
                     /calculated_mol_wt=65244
     Region          <229..>483
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..565
                     /gene="LOC117611558"
                     /coded_by="XM_034339523.2:1..1698"
                     /db_xref="GeneID:117611558"
ORIGIN      
        1 metaspnrrq pgeegivdda kkvqrntvad eqdgqgtarl iagertntqt kiastqtsid
       61 faqtneknlq iteaisnvyr ssscvcferk rstefrgvde epapqwliee alnrgsrser
      121 wtkgkisssk eieyqkiisk lesrlaqike dleealqsks lvkekykrsl enikmkaree
      181 semlqekivq ictsvlekfg pstivdkrks nyqikcrgrl kyhekisskl hkklrmavvr
      241 snklkrelta arkalknkse myesiskcfd elkrdmdtte mnlnnlisen lslrkkiddt
      301 rdwlaernae kvhhasnhlr daqknrelsn lkkkldedka tidqlrnrlm rsesantnkg
      361 fllnsyksql tdlnqeknrl vskinslene isvvrnnnsq lkakisilnn ekvklhsdnk
      421 ksktditeki ktqtvqqese iitakyeeti nsmkikitai snenleyska ikeflrkihn
      481 rrkyeshrns neneneseas ereahetacn ilnmtpeels glingktfns inpwivelnr
      541 ilsenhfsgn lsnflfrkam kklrt
//
LOCUS       XP_076546526             333 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610408 [Osmia lignaria lignaria].
ACCESSION   XP_076546526
VERSION     XP_076546526.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690411.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="uncharacterized protein LOC117610408"
                     /calculated_mol_wt=37864
     CDS             1..333
                     /gene="LOC117610408"
                     /coded_by="XM_076690411.1:1285..2286"
                     /db_xref="GeneID:117610408"
ORIGIN      
        1 mqqasvggck gdpgrvkspk nfamktdiag dpdtrvtvkv eapestemfe vkrepqdeyq
       61 hdyrydrksi apsklevnhd magqtqhqgy rpptsafpns ygfphfygpt mlppafpgpg
      121 arqdkmeeqe rerererere rereqeryrv ssvpgdgafl hhephrfeis dyhskhtgyp
      181 ampyasfrps mqprssyagh qnnsnyrstq yqnrkrkwsg aalrgmhpsm swptwfyrpd
      241 fplgtplppt nhvsnassvp tssplssrtl hldaskgpef ldsskihgqt ptictsirct
      301 vngcscdsft pgkrhirycd rchhgwvlhg krn
//
LOCUS       XP_076546527             297 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305720 [Osmia lignaria lignaria].
ACCESSION   XP_076546527
VERSION     XP_076546527.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690412.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..297
                     /product="uncharacterized protein LOC143305720"
                     /calculated_mol_wt=33509
     CDS             1..297
                     /gene="LOC143305720"
                     /coded_by="XM_076690412.1:495..1388"
                     /db_xref="GeneID:143305720"
ORIGIN      
        1 mmpndsrqdr rckttecspk tlarkiesci vrnaldeeqr aqkalyllka rdrriavlek
       61 rvaelqnlad nihevslrks ssilnperdk eavsdfrser slrtddetek sdsnlsnten
      121 lsktldedcd tvsgsqitvp rlklskssls nsekdifsta tnflvegrkc sassicvsgk
      181 drtfenaalq kdksskeivd srkretkvgg lndqgkidgv ekllseekkg gsvmfmknie
      241 gkvrkrcsfp rssasvpwir arhdcfrrkl rnlelrnakr veklpmgdgr atcttyr
//
LOCUS       XP_034193528             756 aa            linear   INV 03-SEP-2025
DEFINITION  INSC spindle orientation adaptor protein [Osmia lignaria lignaria].
ACCESSION   XP_034193528
VERSION     XP_034193528.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337637.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193528.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..756
                     /product="INSC spindle orientation adaptor protein"
                     /calculated_mol_wt=83887
     Region          288..575
                     /region_name="INSC_LBD"
                     /note="LGN-binding domain (LBD) of protein Inscuteable
                     (INSC) and similar proteins; cl41917"
                     /db_xref="CDD:477865"
     Site            order(288..292,294..296,298..317,329..333,339,343,
                     346..347,350..351,353..354,396,430,447..448,452)
                     /site_type="other"
                     /note="LGN binding site [polypeptide binding]"
                     /db_xref="CDD:439320"
     Region          <498..707
                     /region_name="INSC_LBD"
                     /note="LGN-binding domain (LBD) of protein Inscuteable
                     (INSC) and similar proteins; cl41917"
                     /db_xref="CDD:477865"
     CDS             1..756
                     /gene="insc"
                     /coded_by="XM_034337637.2:887..3157"
                     /db_xref="GeneID:117610361"
ORIGIN      
        1 msafkrhqsk vfwnhmatqd lesvdpfvar ndftrskqnh gpihdipeee aeersssine
       61 sdtfetanvt airicpngne nsqdlyhatt ihegndlmtq mvtmdikded kedeenvavp
      121 inrscsplsq dlrsldsgfs dsersncsel yenetprrrr rrkrvrdrkh gihsragatw
      181 lehetlpdpt ytstpkdsri lprntmvlna ytarsvprmq rteedqvdas aiptsisldd
      241 eclgdflyaa eppedaaepr sgnssittns yeaestsksl qhcrtyrqss svrawlsnla
      301 metetecnnt lqskalprrk hrellsekde mndlktlssl ataaanklli raeqfdkhye
      361 yifdkvshle rgrsekellr aieddafsvl selgappprr iqqtslkgil sqlesiknyv
      421 dsavdtrldf yiekivrgle espkdettva rgslaaltal glagpragss itrcsgiral
      481 ltslltagrt sidfvtaslr alasvcssat aihqfvkdgg peiltdllia ekmsekekme
      541 atalvvqita pwvnalglpy lepfakdlip slnrlvestn cgqtlllvaa afnhlsksrk
      601 cafvilehgt vkkllktvkk stgknvwlme qvaalisela ripearphla earasialvs
      661 flrmrppgle dayrrleita aaaltrlcvd peiarqvvav ggadclpscr iddllteded
      721 qieagllkyt kslrkacrra akqidiakas dystls
//
LOCUS       XP_076546528             756 aa            linear   INV 03-SEP-2025
DEFINITION  INSC spindle orientation adaptor protein [Osmia lignaria lignaria].
ACCESSION   XP_076546528
VERSION     XP_076546528.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690413.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..756
                     /product="INSC spindle orientation adaptor protein"
                     /calculated_mol_wt=83887
     Region          288..575
                     /region_name="INSC_LBD"
                     /note="LGN-binding domain (LBD) of protein Inscuteable
                     (INSC) and similar proteins; cl41917"
                     /db_xref="CDD:477865"
     Site            order(288..292,294..296,298..317,329..333,339,343,
                     346..347,350..351,353..354,396,430,447..448,452)
                     /site_type="other"
                     /note="LGN binding site [polypeptide binding]"
                     /db_xref="CDD:439320"
     Region          <498..707
                     /region_name="INSC_LBD"
                     /note="LGN-binding domain (LBD) of protein Inscuteable
                     (INSC) and similar proteins; cl41917"
                     /db_xref="CDD:477865"
     CDS             1..756
                     /gene="insc"
                     /coded_by="XM_076690413.1:523..2793"
                     /db_xref="GeneID:117610361"
ORIGIN      
        1 msafkrhqsk vfwnhmatqd lesvdpfvar ndftrskqnh gpihdipeee aeersssine
       61 sdtfetanvt airicpngne nsqdlyhatt ihegndlmtq mvtmdikded kedeenvavp
      121 inrscsplsq dlrsldsgfs dsersncsel yenetprrrr rrkrvrdrkh gihsragatw
      181 lehetlpdpt ytstpkdsri lprntmvlna ytarsvprmq rteedqvdas aiptsisldd
      241 eclgdflyaa eppedaaepr sgnssittns yeaestsksl qhcrtyrqss svrawlsnla
      301 metetecnnt lqskalprrk hrellsekde mndlktlssl ataaanklli raeqfdkhye
      361 yifdkvshle rgrsekellr aieddafsvl selgappprr iqqtslkgil sqlesiknyv
      421 dsavdtrldf yiekivrgle espkdettva rgslaaltal glagpragss itrcsgiral
      481 ltslltagrt sidfvtaslr alasvcssat aihqfvkdgg peiltdllia ekmsekekme
      541 atalvvqita pwvnalglpy lepfakdlip slnrlvestn cgqtlllvaa afnhlsksrk
      601 cafvilehgt vkkllktvkk stgknvwlme qvaalisela ripearphla earasialvs
      661 flrmrppgle dayrrleita aaaltrlcvd peiarqvvav ggadclpscr iddllteded
      721 qieagllkyt kslrkacrra akqidiakas dystls
//
LOCUS       XP_034193536             756 aa            linear   INV 03-SEP-2025
DEFINITION  INSC spindle orientation adaptor protein [Osmia lignaria lignaria].
ACCESSION   XP_034193536
VERSION     XP_034193536.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337645.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193536.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..756
                     /product="INSC spindle orientation adaptor protein"
                     /calculated_mol_wt=83887
     Region          288..575
                     /region_name="INSC_LBD"
                     /note="LGN-binding domain (LBD) of protein Inscuteable
                     (INSC) and similar proteins; cl41917"
                     /db_xref="CDD:477865"
     Site            order(288..292,294..296,298..317,329..333,339,343,
                     346..347,350..351,353..354,396,430,447..448,452)
                     /site_type="other"
                     /note="LGN binding site [polypeptide binding]"
                     /db_xref="CDD:439320"
     Region          <498..707
                     /region_name="INSC_LBD"
                     /note="LGN-binding domain (LBD) of protein Inscuteable
                     (INSC) and similar proteins; cl41917"
                     /db_xref="CDD:477865"
     CDS             1..756
                     /gene="insc"
                     /coded_by="XM_034337645.2:365..2635"
                     /db_xref="GeneID:117610361"
ORIGIN      
        1 msafkrhqsk vfwnhmatqd lesvdpfvar ndftrskqnh gpihdipeee aeersssine
       61 sdtfetanvt airicpngne nsqdlyhatt ihegndlmtq mvtmdikded kedeenvavp
      121 inrscsplsq dlrsldsgfs dsersncsel yenetprrrr rrkrvrdrkh gihsragatw
      181 lehetlpdpt ytstpkdsri lprntmvlna ytarsvprmq rteedqvdas aiptsisldd
      241 eclgdflyaa eppedaaepr sgnssittns yeaestsksl qhcrtyrqss svrawlsnla
      301 metetecnnt lqskalprrk hrellsekde mndlktlssl ataaanklli raeqfdkhye
      361 yifdkvshle rgrsekellr aieddafsvl selgappprr iqqtslkgil sqlesiknyv
      421 dsavdtrldf yiekivrgle espkdettva rgslaaltal glagpragss itrcsgiral
      481 ltslltagrt sidfvtaslr alasvcssat aihqfvkdgg peiltdllia ekmsekekme
      541 atalvvqita pwvnalglpy lepfakdlip slnrlvestn cgqtlllvaa afnhlsksrk
      601 cafvilehgt vkkllktvkk stgknvwlme qvaalisela ripearphla earasialvs
      661 flrmrppgle dayrrleita aaaltrlcvd peiarqvvav ggadclpscr iddllteded
      721 qieagllkyt kslrkacrra akqidiakas dystls
//
LOCUS       XP_034193549             534 aa            linear   INV 03-SEP-2025
DEFINITION  chaperonin containing TCP1 subunit 2 [Osmia lignaria lignaria].
ACCESSION   XP_034193549
VERSION     XP_034193549.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337658.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193549.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="chaperonin containing TCP1 subunit 2"
                     /calculated_mol_wt=57719
     Region          9..526
                     /region_name="TCP1_beta"
                     /note="TCP-1 (CTT or eukaryotic type II) chaperonin
                     family, beta subunit. Chaperonins are involved in
                     productive folding of proteins. They share a common
                     general morphology, a double toroid of 2 stacked rings. In
                     contrast to bacterial group I chaperonins (GroEL)...;
                     cd03336"
                     /db_xref="CDD:239452"
     Site            order(14,18,46..49,51,72,74,78,82,85,379,515..521)
                     /site_type="other"
                     /note="ring oligomerisation interface [polypeptide
                     binding]"
                     /db_xref="CDD:239452"
     Site            order(41..43,96,100,164,391,409,449,492,494)
                     /site_type="other"
                     /note="ATP/Mg binding site [chemical binding]"
                     /db_xref="CDD:239452"
     Site            order(429,447,456,458..459,462)
                     /site_type="active"
                     /note="stacking interactions [active]"
                     /db_xref="CDD:239452"
     CDS             1..534
                     /gene="CCT2"
                     /coded_by="XM_034337658.2:219..1823"
                     /db_xref="GeneID:117610369"
ORIGIN      
        1 mvslnpvrif kneaeeekae iarlslfvga iaigdlvkst lgpkgmdkvl vahgrsagqv
       61 qvtndgatil ksvgvdnpaa kilvdmsrvq ddevgdgtts vtvlasellr eaeklidqki
      121 hpqtiiagwr eatnvarkal qntatdnsad perfyedltn iarttlsski laqhkehfsa
      181 lavnavlrlk gsgnlsaiqi ikkrggtlgd sylddgflld kkpgmhqpqr vtdarilian
      241 tpmdtdkikv fgsrvrvdsm akiaeleaae kekmkdkvek iikhgsnvfv nrqliynype
      301 qlfadanima iehadfdgie rlalvtggei vstfdnpemv klgkcdliep vmvgedtllk
      361 fsgvplgeac tviirgatqq ivdeaerslh dalcvlsatv resrivyggg csemimalav
      421 mraaaatpgk eavamesfar alqqlptvia dnagydsaql vsqlraahns gavtmgldme
      481 qgkiscmkkl gvieswavkr qvllsaaeaa emilrvddil raaprkrvrd rgrc
//
LOCUS       XP_034193561             315 aa            linear   INV 03-SEP-2025
DEFINITION  NADH-cytochrome b5 reductase 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193561
VERSION     XP_034193561.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337670.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..315
                     /product="NADH-cytochrome b5 reductase 2 isoform X1"
                     /calculated_mol_wt=35205
     Region          <39..310
                     /region_name="PLN02252"
                     /note="nitrate reductase [NADPH]"
                     /db_xref="CDD:215141"
     CDS             1..315
                     /gene="LOC117610376"
                     /coded_by="XM_034337670.2:231..1178"
                     /db_xref="GeneID:117610376"
ORIGIN      
        1 mneqmnntss nnshalpvla avgtvvvigl afkfykswsg ekkkkspvll vdpvvkyslp
       61 liekeiishd trkfrfglpt pdhvlglpig qhvhltakig edavirsytp vssdddhgyv
      121 dlvikvyfkn vhpkfpeggk lsqyldnlki getidfrgps grliykghgk fsikilrkdp
      181 pveynvkkvv mlaggtgitp mlqliraivk dptdetqasl lfanqtekdi llrneldqia
      241 kdhpdklklw ytldtssenw pystghlnae mikehmfpps pdtivlmcgp ppminfacnp
      301 nldklgydqk lrfay
//
LOCUS       XP_034193570             308 aa            linear   INV 03-SEP-2025
DEFINITION  NADH-cytochrome b5 reductase 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193570
VERSION     XP_034193570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337679.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="NADH-cytochrome b5 reductase 3 isoform X2"
                     /calculated_mol_wt=34427
     Region          <32..303
                     /region_name="PLN02252"
                     /note="nitrate reductase [NADPH]"
                     /db_xref="CDD:215141"
     CDS             1..308
                     /gene="LOC117610376"
                     /coded_by="XM_034337679.2:286..1212"
                     /db_xref="GeneID:117610376"
ORIGIN      
        1 mpnlrslalp vlaavgtvvv iglafkfyks wsgekkkksp vllvdpvvky slpliekeii
       61 shdtrkfrfg lptpdhvlgl pigqhvhlta kigedavirs ytpvssdddh gyvdlvikvy
      121 fknvhpkfpe ggklsqyldn lkigetidfr gpsgrliykg hgkfsikilr kdppveynvk
      181 kvvmlaggtg itpmlqlira ivkdptdetq asllfanqte kdillrneld qiakdhpdkl
      241 klwytldtss enwpystghl naemikehmf ppspdtivlm cgpppminfa cnpnldklgy
      301 dqklrfay
//
LOCUS       XP_034196356            1216 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196356
VERSION     XP_034196356.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340465.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196356.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1216
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X1"
                     /calculated_mol_wt=136786
     Region          42..157
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          280..507
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(446..447,450,456)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          523..588
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          601..653
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          659..755
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          760..869
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          881..935
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          939..1000
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1106..1161
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..1216
                     /gene="LOC117611944"
                     /coded_by="XM_034340465.2:1110..4760"
                     /db_xref="GeneID:117611944"
ORIGIN      
        1 mryslvlqaf ivvvhvvpea rlhrpsnvrg iytlekhvhd heiviprkvt hlgelishnv
       61 thhhhedgpv vhyrlsvggn eyhieltgve nfigpgmvve rrkrgihvrs rpknrsskch
      121 yrgfiqghtn sqvalsacdg lagmlrgehg efwlepvavs peeegvrvee rleedagaeg
      181 rsldglhrns lagrphlvfr rsveqpqlei gaagrtrrrr kkkrklernc gtreprrlte
      241 trlewqtqpg lvqvqgrgrr kqhqtkrwqr srrsisrprh vealvvadtt mmafhqdgdv
      301 etylltimnm vsslyldpti gnfinvvvvr iilveedeae rghlhsasrg tvqanvsfqq
      361 glditvnadr tlynfckwqq klnpaddshp nhhdvailvt redicsrant pcstlgvahv
      421 agmcqpdrsc svnedngitl ahtithelgh nfgmyhdtek igcskrdgdt lhvmtptfev
      481 dtigvawsrc srrditnfld qgkgvclede padndyaypd lppgamynae hqcrlqfgvr
      541 easvcsplqe icsklwcivd gtcttmlhpa apgthcgkhm wcqnqecvpi vdrprqidgg
      601 wgewgswsec srscgagvsi verkcdhpep ahggkfcige rrrykicntq pcpegtpsfr
      661 aiqcsnydgk eykgknytwl pyfdqtepce lyctdteesv ivpwgeaald gtpcnvgtrd
      721 mciagicrkv gcdwtvdsda tedrcgichg dgtqcettgg iydkndgpgy kevvvvpsgs
      781 rnikieeign sknyigigvp nsdkyflngk rqitlageye vagtpalyer drdrekvrip
      841 gpirediavy lisrghyrnf glryeytvpk kepdrapeys wlfsdwslct atcgggtqts
      901 kavcnekksg ivedqfcdgi ekpesilrec nlnpcparww igpwqmcpvt cgegalrkrs
      961 vmcvssgmgp nrsdlalpdr dcnrdvrpee vspcpnlppc sstepplivy adnkdasfyn
     1021 vssndqdgit ivdgltteep eilefdnvvd enpdnsmynt kskwivskws hctngkrsrk
     1081 vscsvqgdcs lenkpvsvel cqggrwvtgr wgscnatclv rigikrreve crdritells
     1141 ndcnperrpi dtrrcyhrrq ctndkseckd tivpasmcat ykrmcnvsli vqekccatcf
     1201 kkrrhgrqnk rhildd
//
LOCUS       XP_076546529            1216 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546529
VERSION     XP_076546529.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690414.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1216
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X1"
                     /calculated_mol_wt=136786
     Region          42..157
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          280..507
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(446..447,450,456)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          523..588
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          601..653
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          659..755
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          760..869
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          881..935
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          939..1000
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1106..1161
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..1216
                     /gene="LOC117611944"
                     /coded_by="XM_076690414.1:1288..4938"
                     /db_xref="GeneID:117611944"
ORIGIN      
        1 mryslvlqaf ivvvhvvpea rlhrpsnvrg iytlekhvhd heiviprkvt hlgelishnv
       61 thhhhedgpv vhyrlsvggn eyhieltgve nfigpgmvve rrkrgihvrs rpknrsskch
      121 yrgfiqghtn sqvalsacdg lagmlrgehg efwlepvavs peeegvrvee rleedagaeg
      181 rsldglhrns lagrphlvfr rsveqpqlei gaagrtrrrr kkkrklernc gtreprrlte
      241 trlewqtqpg lvqvqgrgrr kqhqtkrwqr srrsisrprh vealvvadtt mmafhqdgdv
      301 etylltimnm vsslyldpti gnfinvvvvr iilveedeae rghlhsasrg tvqanvsfqq
      361 glditvnadr tlynfckwqq klnpaddshp nhhdvailvt redicsrant pcstlgvahv
      421 agmcqpdrsc svnedngitl ahtithelgh nfgmyhdtek igcskrdgdt lhvmtptfev
      481 dtigvawsrc srrditnfld qgkgvclede padndyaypd lppgamynae hqcrlqfgvr
      541 easvcsplqe icsklwcivd gtcttmlhpa apgthcgkhm wcqnqecvpi vdrprqidgg
      601 wgewgswsec srscgagvsi verkcdhpep ahggkfcige rrrykicntq pcpegtpsfr
      661 aiqcsnydgk eykgknytwl pyfdqtepce lyctdteesv ivpwgeaald gtpcnvgtrd
      721 mciagicrkv gcdwtvdsda tedrcgichg dgtqcettgg iydkndgpgy kevvvvpsgs
      781 rnikieeign sknyigigvp nsdkyflngk rqitlageye vagtpalyer drdrekvrip
      841 gpirediavy lisrghyrnf glryeytvpk kepdrapeys wlfsdwslct atcgggtqts
      901 kavcnekksg ivedqfcdgi ekpesilrec nlnpcparww igpwqmcpvt cgegalrkrs
      961 vmcvssgmgp nrsdlalpdr dcnrdvrpee vspcpnlppc sstepplivy adnkdasfyn
     1021 vssndqdgit ivdgltteep eilefdnvvd enpdnsmynt kskwivskws hctngkrsrk
     1081 vscsvqgdcs lenkpvsvel cqggrwvtgr wgscnatclv rigikrreve crdritells
     1141 ndcnperrpi dtrrcyhrrq ctndkseckd tivpasmcat ykrmcnvsli vqekccatcf
     1201 kkrrhgrqnk rhildd
//
LOCUS       XP_076546530            1216 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546530
VERSION     XP_076546530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690415.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1216
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X1"
                     /calculated_mol_wt=136786
     Region          42..157
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          280..507
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(446..447,450,456)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          523..588
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          601..653
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          659..755
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          760..869
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          881..935
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          939..1000
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1106..1161
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..1216
                     /gene="LOC117611944"
                     /coded_by="XM_076690415.1:367..4017"
                     /db_xref="GeneID:117611944"
ORIGIN      
        1 mryslvlqaf ivvvhvvpea rlhrpsnvrg iytlekhvhd heiviprkvt hlgelishnv
       61 thhhhedgpv vhyrlsvggn eyhieltgve nfigpgmvve rrkrgihvrs rpknrsskch
      121 yrgfiqghtn sqvalsacdg lagmlrgehg efwlepvavs peeegvrvee rleedagaeg
      181 rsldglhrns lagrphlvfr rsveqpqlei gaagrtrrrr kkkrklernc gtreprrlte
      241 trlewqtqpg lvqvqgrgrr kqhqtkrwqr srrsisrprh vealvvadtt mmafhqdgdv
      301 etylltimnm vsslyldpti gnfinvvvvr iilveedeae rghlhsasrg tvqanvsfqq
      361 glditvnadr tlynfckwqq klnpaddshp nhhdvailvt redicsrant pcstlgvahv
      421 agmcqpdrsc svnedngitl ahtithelgh nfgmyhdtek igcskrdgdt lhvmtptfev
      481 dtigvawsrc srrditnfld qgkgvclede padndyaypd lppgamynae hqcrlqfgvr
      541 easvcsplqe icsklwcivd gtcttmlhpa apgthcgkhm wcqnqecvpi vdrprqidgg
      601 wgewgswsec srscgagvsi verkcdhpep ahggkfcige rrrykicntq pcpegtpsfr
      661 aiqcsnydgk eykgknytwl pyfdqtepce lyctdteesv ivpwgeaald gtpcnvgtrd
      721 mciagicrkv gcdwtvdsda tedrcgichg dgtqcettgg iydkndgpgy kevvvvpsgs
      781 rnikieeign sknyigigvp nsdkyflngk rqitlageye vagtpalyer drdrekvrip
      841 gpirediavy lisrghyrnf glryeytvpk kepdrapeys wlfsdwslct atcgggtqts
      901 kavcnekksg ivedqfcdgi ekpesilrec nlnpcparww igpwqmcpvt cgegalrkrs
      961 vmcvssgmgp nrsdlalpdr dcnrdvrpee vspcpnlppc sstepplivy adnkdasfyn
     1021 vssndqdgit ivdgltteep eilefdnvvd enpdnsmynt kskwivskws hctngkrsrk
     1081 vscsvqgdcs lenkpvsvel cqggrwvtgr wgscnatclv rigikrreve crdritells
     1141 ndcnperrpi dtrrcyhrrq ctndkseckd tivpasmcat ykrmcnvsli vqekccatcf
     1201 kkrrhgrqnk rhildd
//
LOCUS       XP_034196358            1197 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196358
VERSION     XP_034196358.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340467.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196358.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1197
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X2"
                     /calculated_mol_wt=134752
     Region          42..157
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          280..488
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(427..428,431,437)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          504..569
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          582..634
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          640..736
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          741..850
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          862..916
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          920..981
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1087..1142
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..1197
                     /gene="LOC117611944"
                     /coded_by="XM_034340467.2:1110..4703"
                     /db_xref="GeneID:117611944"
ORIGIN      
        1 mryslvlqaf ivvvhvvpea rlhrpsnvrg iytlekhvhd heiviprkvt hlgelishnv
       61 thhhhedgpv vhyrlsvggn eyhieltgve nfigpgmvve rrkrgihvrs rpknrsskch
      121 yrgfiqghtn sqvalsacdg lagmlrgehg efwlepvavs peeegvrvee rleedagaeg
      181 rsldglhrns lagrphlvfr rsveqpqlei gaagrtrrrr kkkrklernc gtreprrlte
      241 trlewqtqpg lvqvqgrgrr kqhqtkrwqr srrsisrprh vealvvadtt mmafhqdgdv
      301 etylltimnm vsslyldpti gnfinvvvvr iilveedeae qglditvnad rtlynfckwq
      361 qklnpaddsh pnhhdvailv tredicsran tpcstlgvah vagmcqpdrs csvnedngit
      421 lahtithelg hnfgmyhdte kigcskrdgd tlhvmtptfe vdtigvawsr csrrditnfl
      481 dqgkgvcled epadndyayp dlppgamyna ehqcrlqfgv reasvcsplq eicsklwciv
      541 dgtcttmlhp aapgthcgkh mwcqnqecvp ivdrprqidg gwgewgswse csrscgagvs
      601 iverkcdhpe pahggkfcig errrykicnt qpcpegtpsf raiqcsnydg keykgknytw
      661 lpyfdqtepc elyctdtees vivpwgeaal dgtpcnvgtr dmciagicrk vgcdwtvdsd
      721 atedrcgich gdgtqcettg giydkndgpg ykevvvvpsg srnikieeig nsknyigigv
      781 pnsdkyflng krqitlagey evagtpalye rdrdrekvri pgpirediav ylisrghyrn
      841 fglryeytvp kkepdrapey swlfsdwslc tatcgggtqt skavcnekks givedqfcdg
      901 iekpesilre cnlnpcparw wigpwqmcpv tcgegalrkr svmcvssgmg pnrsdlalpd
      961 rdcnrdvrpe evspcpnlpp cssteppliv yadnkdasfy nvssndqdgi tivdglttee
     1021 peilefdnvv denpdnsmyn tkskwivskw shctngkrsr kvscsvqgdc slenkpvsve
     1081 lcqggrwvtg rwgscnatcl vrigikrrev ecrdritell sndcnperrp idtrrcyhrr
     1141 qctndkseck dtivpasmca tykrmcnvsl ivqekccatc fkkrrhgrqn krhildd
//
LOCUS       XP_034196359            1147 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196359
VERSION     XP_034196359.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340468.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196359.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1147
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X1"
                     /calculated_mol_wt=129224
     Region          73..199
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          263..472
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(410..411,414,420)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          489..553
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          566..618
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          <676..721
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          723..836
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          849..903
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          907..967
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..1147
                     /gene="LOC117611945"
                     /coded_by="XM_034340468.2:27..3470"
                     /db_xref="GeneID:117611945"
ORIGIN      
        1 mnefqalltl lgilvtvdgd ildkysrgsa kvyhgrytrd ihepellvpr rvfedgnfnt
       61 yslpnfydrq eiserrkrsi edreeeteme klhlilpfkg iehhvelspy hdfispdmvi
      121 etrgsgirdn lnealrfkrv pdrqchyrgi irghhgsraa lslcdgvvgy vqtnhgryfi
      181 epmndvepee dgqhvhiayr reaphekken qdgtpkrhct vgddweaawa eqlakrqkrl
      241 mednsigtkr enqvsfgths ihryieiglv adrrfldfhn nsdyeqyllt imnmvsdfyh
      301 dksvgnqidv vvvrmiylek ekeeidllis paaeetlasf akwadkmnpk dsthpnhhdi
      361 gvlvtrydic segtncelmg layvaaacds traasineds glllgiviah evghvmgcsh
      421 depaisgcqs qdkdesyfim spivfiftir wsscskrfit sflesglgec lnddpknppn
      481 kfaypnmlpg amydadfqcr mmfpeskhcp nsrlrvcdkl wcntnascys rgapmadgtk
      541 cgekkwcihk qcvdmgtrpt avhggwgkwg pagscsrtcg gglkfterec dnpppanggr
      601 ycigerkkva icnttpcdpk kpsfravqca aynnrkvlsd glhqwiayyg tnvdpcalyc
      661 ineqrifvki tpmvndstpc kpgtnymcis gkcrkvgcdw gldsnaiedg cgickgdgtk
      721 ckpvegffnl tearvrfeki vtipkgargi hveelkdhrn ilavryekkd vycingdmse
      781 arsgdydcag avliyqhpep hreeilikgp itedirieyl fhivdsnpgv ryvyyvtstd
      841 psytpkymwd fmewsdcnak cgggtmisea tcseehggkv sptfckdipk pdpksricnp
      901 npcpakwrvs qwskcsacds kkgtrhrkvq cvkpaarege ddvqanfnac kgrvpkqeee
      961 cignrpckkt cakktrdade nvetkkgrql ipeekqsrmi dkfvdlslar ylakshgdvp
     1021 ieanefdrtg dladfrqllr dwsivdedkk krgtcdkdlt tpqpgsiikd svpignvvle
     1081 evpyieeslq anlsdkafqq sgdligasid tshekvykga kavamieqla ghrettpltr
     1141 ttnqtnt
//
LOCUS       XP_034196360            1059 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196360
VERSION     XP_034196360.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340469.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196360.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1059
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X2"
                     /calculated_mol_wt=118789
     Region          2..111
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          175..384
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(322..323,326,332)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          401..465
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          478..530
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          <588..633
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          635..748
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          761..815
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          819..>854
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..1059
                     /gene="LOC117611945"
                     /coded_by="XM_034340469.2:278..3457"
                     /db_xref="GeneID:117611945"
ORIGIN      
        1 meklhlilpf kgiehhvels pyhdfispdm vietrgsgir dnlnealrfk rvpdrqchyr
       61 giirghhgsr aalslcdgvv gyvqtnhgry fiepmndvep eedgqhvhia yrreaphekk
      121 enqdgtpkrh ctvgddweaa waeqlakrqk rlmednsigt krenqvsfgt hsihryieig
      181 lvadrrfldf hnnsdyeqyl ltimnmvsdf yhdksvgnqi dvvvvrmiyl ekekeeidll
      241 ispaaeetla sfakwadkmn pkdsthpnhh digvlvtryd icsegtncel mglayvaaac
      301 dstraasine dsglllgivi ahevghvmgc shdepaisgc qsqdkdesyf imspivfift
      361 irwsscskrf itsflesglg eclnddpknp pnkfaypnml pgamydadfq crmmfpeskh
      421 cpnsrlrvcd klwcntnasc ysrgapmadg tkcgekkwci hkqcvdmgtr ptavhggwgk
      481 wgpagscsrt cggglkfter ecdnpppang grycigerkk vaicnttpcd pkkpsfravq
      541 caaynnrkvl sdglhqwiay ygtnvdpcal ycineqrifv kitpmvndst pckpgtnymc
      601 isgkcrkvgc dwgldsnaie dgcgickgdg tkckpvegff nltearvrfe kivtipkgar
      661 gihveelkdh rnilavryek kdvycingdm searsgdydc agavliyqhp ephreeilik
      721 gpitedirie ylfhivdsnp gvryvyyvts tdpsytpkym wdfmewsdcn akcgggtmis
      781 eatcseehgg kvsptfckdi pkpdpksric npnpcpakwr vsqwskcsac dskkgtrhrk
      841 vqcvkpaare geddvqanfn ackgrvpkqe eecignrpck ktcakktrda denvetkkgr
      901 qlipeekqsr midkfvdlsl arylakshgd vpieanefdr tgdladfrql lrdwsivded
      961 kkkrgtcdkd lttpqpgsii kdsvpignvv leevpyiees lqanlsdkaf qqsgdligas
     1021 idtshekvyk gakavamieq laghrettpl trttnqtnt
//
LOCUS       XP_034196361            1049 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196361
VERSION     XP_034196361.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340470.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196361.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1049
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X1"
                     /calculated_mol_wt=118522
     Region          43..194
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          239..451
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     Site            order(386..387,390,396)
                     /site_type="active"
                     /db_xref="CDD:238124"
     Region          467..531
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          544..596
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          <659..705
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          722..809
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          823..877
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..1049
                     /gene="LOC117611946"
                     /coded_by="XM_034340470.2:1011..4160"
                     /db_xref="GeneID:117611946"
ORIGIN      
        1 mikhpsmflf vyvllfvppr rggfangyrg rytrdvefsd eyeivvprkv rsdgkflshq
       61 iphhfkrsfy aarsnrdivp ddsvhyrlki gdkehhlele pnhrlvapga vieeqrgscn
      121 cknnedflkn iklqrmcdaq chylgrvrgq sensalstcy glagyirtkr wwytiepltg
      181 hdftkeaehp hvvykkrpde fgrnsqmlcn vtgnmgksia krafssqkrh psrkqarsyt
      241 lelllvldki vidyhkdfdv qnyvltllnm aagllhdvsl gilmelsivr iirmqiqdde
      301 msltatknpe ktlkyfqewq qtinpsddlh pnhhdcaili tksnicdtss scaftgtsti
      361 agtcdplkga avvkdvglrt gyhlahqigh tlgmshdiqe kngcpgiiqr rkgyiettvm
      421 ypgslyvtrk wskcsrnsln syieagfgfc ledkpyihef pstellpgvm ynaddqcrle
      481 yrkdarycdi gisceslrca ipgrgcvsar kplaegtscg knrwcygmkc lmvgerpgvv
      541 dgdwggwspw srcsrscgsg vafsvrrcsn pspsnggayc rgarkrhkic atnpcdvdap
      601 sfrdvqcaef nswifpedgn vhrwtaynlp enliasenpc alyclserkv vaslrpkvid
      661 gttcyrgird icvegicrei pcdlnmesna iedvcgvcrg ngtscrlkea iitiemasqp
      721 kkivdvpsga tnirveetep tksrimvrsk dgkslidgdr lgmfevagsk awlgtirpnq
      781 ealnipgpvm kdlvilvqpk envtvkysyg vkeksprkpe fswdfvdwek csancgpgeq
      841 iskprclekv aglvdemfck nirkpeakvr pchrapclpr wmigdwqgct scllgcekrr
      901 avkcvrpvgn aeqdvdiiad sycqapkpke rescsdrkkr dddrtreern kndpnrkste
      961 trterkqdak esqivkkgev vidkediknl tltiflerde etgdlnfpkd feprpsanst
     1021 eftlvgmdai ryiqkiqens giskkhtyl
//
LOCUS       XP_076546531             914 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546531
VERSION     XP_076546531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690416.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..914
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X2"
                     /calculated_mol_wt=102646
     Region          <3..59
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          104..316
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     Site            order(251..252,255,261)
                     /site_type="active"
                     /db_xref="CDD:238124"
     Region          332..396
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          409..461
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          <524..570
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          587..674
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          688..742
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..914
                     /gene="LOC117611946"
                     /coded_by="XM_076690416.1:1390..4134"
                     /db_xref="GeneID:117611946"
ORIGIN      
        1 mcdaqchylg rvrgqsensa lstcyglagy irtkrwwyti epltghdftk eaehphvvyk
       61 krpdefgrns qmlcnvtgnm gksiakrafs sqkrhpsrkq arsytlelll vldkividyh
      121 kdfdvqnyvl tllnmaagll hdvslgilme lsivriirmq iqddemslta tknpektlky
      181 fqewqqtinp sddlhpnhhd cailitksni cdtssscaft gtstiagtcd plkgaavvkd
      241 vglrtgyhla hqightlgms hdiqekngcp giiqrrkgyi ettvmypgsl yvtrkwskcs
      301 rnslnsyiea gfgfcledkp yihefpstel lpgvmynadd qcrleyrkda rycdigisce
      361 slrcaipgrg cvsarkplae gtscgknrwc ygmkclmvge rpgvvdgdwg gwspwsrcsr
      421 scgsgvafsv rrcsnpspsn ggaycrgark rhkicatnpc dvdapsfrdv qcaefnswif
      481 pedgnvhrwt aynlpenlia senpcalycl serkvvaslr pkvidgttcy rgirdicveg
      541 icreipcdln mesnaiedvc gvcrgngtsc rlkeaiitie masqpkkivd vpsgatnirv
      601 eeteptksri mvrskdgksl idgdrlgmfe vagskawlgt irpnqealni pgpvmkdlvi
      661 lvqpkenvtv kysygvkeks prkpefswdf vdwekcsanc gpgeqiskpr clekvaglvd
      721 emfcknirkp eakvrpchra pclprwmigd wqgctscllg cekrravkcv rpvgnaeqdv
      781 diiadsycqa pkpkerescs drkkrdddrt reernkndpn rkstetrter kqdakesqiv
      841 kkgevvidke diknltltif lerdeetgdl nfpkdfeprp sansteftlv gmdairyiqk
      901 iqensgiskk htyl
//
LOCUS       XP_076546532             914 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 7
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546532
VERSION     XP_076546532.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690417.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..914
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 7 isoform X2"
                     /calculated_mol_wt=102646
     Region          <3..59
                     /region_name="Pep_M12B_propep"
                     /note="Reprolysin family propeptide; pfam01562"
                     /db_xref="CDD:460254"
     Region          104..316
                     /region_name="ZnMc"
                     /note="Zinc-dependent metalloprotease. This super-family
                     of metalloproteases contains two major branches, the
                     astacin-like proteases and the adamalysin/reprolysin-like
                     proteases. Both branches have wide phylogenetic
                     distribution, and contain sub-families, which...; cl00064"
                     /db_xref="CDD:469599"
     Site            order(251..252,255,261)
                     /site_type="active"
                     /db_xref="CDD:238124"
     Region          332..396
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          409..461
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          <524..570
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          587..674
                     /region_name="ADAMTS_spacer1"
                     /note="ADAM-TS Spacer 1; pfam05986"
                     /db_xref="CDD:461796"
     Region          688..742
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     CDS             1..914
                     /gene="LOC117611946"
                     /coded_by="XM_076690417.1:875..3619"
                     /db_xref="GeneID:117611946"
ORIGIN      
        1 mcdaqchylg rvrgqsensa lstcyglagy irtkrwwyti epltghdftk eaehphvvyk
       61 krpdefgrns qmlcnvtgnm gksiakrafs sqkrhpsrkq arsytlelll vldkividyh
      121 kdfdvqnyvl tllnmaagll hdvslgilme lsivriirmq iqddemslta tknpektlky
      181 fqewqqtinp sddlhpnhhd cailitksni cdtssscaft gtstiagtcd plkgaavvkd
      241 vglrtgyhla hqightlgms hdiqekngcp giiqrrkgyi ettvmypgsl yvtrkwskcs
      301 rnslnsyiea gfgfcledkp yihefpstel lpgvmynadd qcrleyrkda rycdigisce
      361 slrcaipgrg cvsarkplae gtscgknrwc ygmkclmvge rpgvvdgdwg gwspwsrcsr
      421 scgsgvafsv rrcsnpspsn ggaycrgark rhkicatnpc dvdapsfrdv qcaefnswif
      481 pedgnvhrwt aynlpenlia senpcalycl serkvvaslr pkvidgttcy rgirdicveg
      541 icreipcdln mesnaiedvc gvcrgngtsc rlkeaiitie masqpkkivd vpsgatnirv
      601 eeteptksri mvrskdgksl idgdrlgmfe vagskawlgt irpnqealni pgpvmkdlvi
      661 lvqpkenvtv kysygvkeks prkpefswdf vdwekcsanc gpgeqiskpr clekvaglvd
      721 emfcknirkp eakvrpchra pclprwmigd wqgctscllg cekrravkcv rpvgnaeqdv
      781 diiadsycqa pkpkerescs drkkrdddrt reernkndpn rkstetrter kqdakesqiv
      841 kkgevvidke diknltltif lerdeetgdl nfpkdfeprp sansteftlv gmdairyiqk
      901 iqensgiskk htyl
//
LOCUS       XP_034196365             285 aa            linear   INV 03-SEP-2025
DEFINITION  zwei Ig domain protein zig-8-like [Osmia lignaria lignaria].
ACCESSION   XP_034196365
VERSION     XP_034196365.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340474.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..285
                     /product="zwei Ig domain protein zig-8-like"
                     /calculated_mol_wt=31849
     Region          63..156
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          74..78
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          87..91
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          119..127
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          <175..244
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     CDS             1..285
                     /gene="LOC117611949"
                     /coded_by="XM_034340474.2:50..907"
                     /db_xref="GeneID:117611949"
ORIGIN      
        1 mnlrhnvdls gfcvpvlakm icifffslll hegtvhssrl vetdafqews tldsshyfdt
       61 tipsnvtglv gdtvelmckv knlgnktvsw mrhrdihllt vsnyiytsdq riqtihksnt
      121 eewvlriqnp qkkdsgiyec qisttppvgh rvyltvvepe teisggpdlf inkystinlt
      181 clvkfapepp stivwshnhe pinfespkgg islitekgpv tssrlliqka iekdsglytc
      241 spnntrpnsv rvhivdehpa amhhgaghri aatllhflll ifgii
//
LOCUS       XP_076546533             525 aa            linear   INV 03-SEP-2025
DEFINITION  myrosinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546533
VERSION     XP_076546533.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690418.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="myrosinase 1 isoform X1"
                     /calculated_mol_wt=60534
     Region          65..519
                     /region_name="Glyco_hydro"
                     /note="Glycosyl hydrolases; cl23725"
                     /db_xref="CDD:474034"
     CDS             1..525
                     /gene="LOC117610474"
                     /coded_by="XM_076690418.1:16..1593"
                     /db_xref="GeneID:117610474"
ORIGIN      
        1 msevrslvaa atflnhisik eaesmasvyr irnmfsmlpl gssiflclvi sasgklveqn
       61 ekylqfppnf llgaataayq iegawnvsdk gesvwdrfsh ergnhvyhne tgdvaansyy
      121 kykedvailk nlgfksyrls lswprilptg ypnkisrdgi qyyhkviddl landiepmvt
      181 lyhwdhpqil edaggwtnse mvewfadyar vvfkefapki krfipvnepq eicrngylyg
      241 nhapgkrlhg igeylclhnv lkahartyri yekefkeqyk gevgilnnvf gyfpktpkde
      301 aaaeiafqfn ggwvlnpiyn gdypavmkef vakrsleqgf trsrlpefga ewiqyirgta
      361 dflalnhyts rivengtyge vpsqdndqmv lvsmdeswks saskwlkvvp egfrlclrkl
      421 aehygnppiy itesgfsdrg tlndddridy yreylkqmll ahyvdgvnvr gyllwslldn
      481 fewdkgyner fgivsidfnd pnrprtlkks atwwqkiisd rkldq
//
LOCUS       XP_034193813             492 aa            linear   INV 03-SEP-2025
DEFINITION  myrosinase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193813
VERSION     XP_034193813.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337922.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193813.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..492
                     /product="myrosinase 1 isoform X2"
                     /calculated_mol_wt=56857
     Region          32..486
                     /region_name="Glyco_hydro"
                     /note="Glycosyl hydrolases; cl23725"
                     /db_xref="CDD:474034"
     CDS             1..492
                     /gene="LOC117610474"
                     /coded_by="XM_034337922.2:189..1667"
                     /db_xref="GeneID:117610474"
ORIGIN      
        1 mfsmlplgss iflclvisas gklveqneky lqfppnfllg aataayqieg awnvsdkges
       61 vwdrfsherg nhvyhnetgd vaansyykyk edvailknlg fksyrlslsw prilptgypn
      121 kisrdgiqyy hkviddllan diepmvtlyh wdhpqileda ggwtnsemve wfadyarvvf
      181 kefapkikrf ipvnepqeic rngylygnha pgkrlhgige ylclhnvlka hartyriyek
      241 efkeqykgev gilnnvfgyf pktpkdeaaa eiafqfnggw vlnpiyngdy pavmkefvak
      301 rsleqgftrs rlpefgaewi qyirgtadfl alnhytsriv engtygevps qdndqmvlvs
      361 mdeswkssas kwlkvvpegf rlclrklaeh ygnppiyite sgfsdrgtln dddridyyre
      421 ylkqmllahy vdgvnvrgyl lwslldnfew dkgynerfgi vsidfndpnr prtlkksatw
      481 wqkiisdrkl dq
//
LOCUS       XP_034193805             528 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-glucosyltransferase 2-like [Osmia lignaria lignaria].
ACCESSION   XP_034193805
VERSION     XP_034193805.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337914.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193805.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..528
                     /product="UDP-glucosyltransferase 2-like"
                     /calculated_mol_wt=60504
     Region          7..498
                     /region_name="Glycosyltransferase_GTB-type"
                     /note="glycosyltransferase family 1 and related proteins
                     with GTB topology; cl10013"
                     /db_xref="CDD:471961"
     Site            order(36..37,39,187,208..209,213,245,304,368,370,372..373,
                     376)
                     /site_type="active"
                     /db_xref="CDD:340817"
     Site            order(36,304,368,370,372..373)
                     /site_type="active"
                     /note="TDP-binding site [active]"
                     /db_xref="CDD:340817"
     Site            37
                     /site_type="active"
                     /note="acceptor substrate-binding pocket [active]"
                     /db_xref="CDD:340817"
     CDS             1..528
                     /gene="LOC117610467"
                     /coded_by="XM_034337914.2:452..2038"
                     /db_xref="GeneID:117610467"
ORIGIN      
        1 mklnvavvlf vvcavcilee trgarilavv ptpsyshqip yrklwtelhk rghdvvlvta
       61 npianmtskn ftqidisgsy kdlqalnflk lrfegvnwit ftenylfdls ttfaesvlnn
      121 telrrlcapg snekfdvvlt ellympsvpa layrfdapli glsslglvai hehdlggfvl
      181 psheytweme antgsnlsfw rrlwnyvslw ritysiyrdl iprhqeiaeh ylgmrlpplt
      241 diarntslif vnqadaltpa rprlpnmitf tsfhvnenpa plpkdlqrfl aeakegfiyf
      301 slgsnarsad ipmavqqvff dvfaklpykv vwkyendlpv kldnvyaskw lpqqsilahp
      361 niklfiyqgg lqsteeavnf avpllgfpil adqdyqvari dtlrvgkrle iltvtreeld
      421 saireiitnk eykermlhlr dlvrdnpydl tdnlvwwtey virhkgaphl rstlagqpwy
      481 qrydidivmf ltivvfvial klvnivatla vhlyirflss avqkqkis
//
LOCUS       XP_034195872             402 aa            linear   INV 03-SEP-2025
DEFINITION  innexin 7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195872
VERSION     XP_034195872.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339981.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="innexin 7 isoform X1"
                     /calculated_mol_wt=45519
     Region          28..382
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..402
                     /gene="Inx7"
                     /coded_by="XM_034339981.2:54..1262"
                     /db_xref="GeneID:117611761"
ORIGIN      
        1 mstvlatfav lkdhvklkvt qdyvaidsmi fklhyrvtfl illvatllvt arqfigehir
       61 ciggagmsdd qvkvintfcf ftstytvvkh mnatavglge vahpgvgpas rddpvvhhay
      121 yqwvpfvlfi qaicfyaphy lwrnveggrl ktlvsglhma slalreksfk tdngisvlst
      181 serddkirqi rvaflnrvhl nrpwayylgl cevlnfinvl vqiyvtdwfl ggaflglgqa
      241 vaagsangem dpldvvfpkv tkcvfhkyga sgtiqnhdal cvmalnivne kiylflwywf
      301 iilavltglg llwriltmll harstlfnkm vfsmacpgrf spwnvltvtn echygdwlfl
      361 yyiaknldny vfkellqkla edlqerrtlk qnppqeeqpl th
//
LOCUS       XP_034195873             376 aa            linear   INV 03-SEP-2025
DEFINITION  innexin 7 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195873
VERSION     XP_034195873.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339982.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="innexin 7 isoform X2"
                     /calculated_mol_wt=42454
     Region          28..356
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..376
                     /gene="Inx7"
                     /coded_by="XM_034339982.2:54..1184"
                     /db_xref="GeneID:117611761"
ORIGIN      
        1 mstvlatfav lkdhvklkvt qdyvaidsmi fklhyrvtfl illvatllvt arqfigehir
       61 ciggagmsdd qvkvintfcf ftstytvvkh mnatavglge vahpgvgpas rddpvvhhay
      121 yqwvpfvlfi qaicfyaphy lwrnveggrl ktlvsglhma slalreksfk tdngisvlst
      181 serddkirqi rvaflnrvhl nrpwayylgl cevlnfinvl vqiyvtdwfl ggaflglgqa
      241 vaagsangem dpldvvfpkv tkcvfhkyga sgtiqnhdal cvmalnivne kiylflwstl
      301 fnkmvfsmac pgrfspwnvl tvtnechygd wlflyyiakn ldnyvfkell qklaedlqer
      361 rtlkqnppqe eqplth
//
LOCUS       XP_034195874             361 aa            linear   INV 03-SEP-2025
DEFINITION  optic ganglion reduced [Osmia lignaria lignaria].
ACCESSION   XP_034195874
VERSION     XP_034195874.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339983.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..361
                     /product="optic ganglion reduced"
                     /calculated_mol_wt=42086
     Region          21..352
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..361
                     /gene="ogre"
                     /coded_by="XM_034339983.2:926..2011"
                     /db_xref="GeneID:117611762"
ORIGIN      
        1 mykllgglgt flkwqeittd smvfrlhnhf tavllftcsm vitatqyvgn pincivqglp
       61 phpvntycwi tstftmpdaf nrqvgievah pgvandfgdv darkyytyyq wvcfvlffqa
      121 clcyvpqwlw nmwegglisk lvmgmnhgld kqenirkkks vlmdylmnhi rshnmyvyry
      181 ficealclvn ifvqlylmnr ffdgeflsyg lrvlqfsevp qeervdpmvy vfprvtkclf
      241 ykygasgtiq qhdslcilpl nivnektyif iwfwftilsi lllglmvyra aiifapavrp
      301 rllhlssrll cietchsisk kidlgdwwll yilssnmdsl iyrdflqelt kkmgdrqsam
      361 a
//
LOCUS       XP_034193841             717 aa            linear   INV 03-SEP-2025
DEFINITION  sodium- and chloride-dependent glycine transporter 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034193841
VERSION     XP_034193841.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337950.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..717
                     /product="sodium- and chloride-dependent glycine
                     transporter 1 isoform X1"
                     /calculated_mol_wt=79733
     Region          41..587
                     /region_name="SNF"
                     /note="Sodium:neurotransmitter symporter family;
                     pfam00209"
                     /db_xref="CDD:395155"
     CDS             1..717
                     /gene="LOC117610479"
                     /coded_by="XM_034337950.2:745..2898"
                     /db_xref="GeneID:117610479"
ORIGIN      
        1 meessprirs ylecwdqned kysvansqap lqagedehpe rgtwtgkfdf llsllgysvg
       61 lgnvwrfpyl cysngggafl ipftimliia glplmfmels lgqyaslgpv aaykrfcpll
      121 rglgygmvlv ssvvmlyynl iiawtlyyif dsvagagnlp wskcespwst edcfmpeaae
      181 acasqnmsyf kmkclnstqm asmgltieni tsairrppae eyfnnhvlgm ssgieetgsi
      241 rpsmavnlll awiivflcls kgvqssgkvv yftalfpyfv livlfvrgil lpgasegilf
      301 yltpdwrrlm sakvwgdaav qiffalspaw gglitlssyn kfnnncykds livavsnigt
      361 sffagfvifs vigflaheld vpvasvvdqg aglafivype vvarlpvapv wsllffvmll
      421 slgldsqfal metvttaild afpalrnykf wvvfaaavfg yaggiifttn agmywlqlmd
      481 kyaanwsvll iaiaecilva wiygadrfld dvqqmigprs rlwrffwtwm wkvitpatlf
      541 filffnwvey eplkyggyvy prwadivgwv vgmipilviv fvainqlrdp rrrsaydddd
      601 dddddldgrp ssqrsgkgkt kksknksvww rakmllqpts dwgpaarnsl tpsrtlthtp
      661 spgppggyds vrdtivlvng qpmmvqpstt sgtaqklpgp silknssktd litsqqv
//
LOCUS       XP_034193849             716 aa            linear   INV 03-SEP-2025
DEFINITION  sodium- and chloride-dependent glycine transporter 1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034193849
VERSION     XP_034193849.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337958.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..716
                     /product="sodium- and chloride-dependent glycine
                     transporter 1 isoform X2"
                     /calculated_mol_wt=79605
     Region          40..586
                     /region_name="SNF"
                     /note="Sodium:neurotransmitter symporter family;
                     pfam00209"
                     /db_xref="CDD:395155"
     CDS             1..716
                     /gene="LOC117610479"
                     /coded_by="XM_034337958.2:746..2896"
                     /db_xref="GeneID:117610479"
ORIGIN      
        1 meessprirs ylecwdnedk ysvansqapl qagedehper gtwtgkfdfl lsllgysvgl
       61 gnvwrfpylc ysngggafli pftimliiag lplmfmelsl gqyaslgpva aykrfcpllr
      121 glgygmvlvs svvmlyynli iawtlyyifd svagagnlpw skcespwste dcfmpeaaea
      181 casqnmsyfk mkclnstqma smgltienit sairrppaee yfnnhvlgms sgieetgsir
      241 psmavnllla wiivflclsk gvqssgkvvy ftalfpyfvl ivlfvrgill pgasegilfy
      301 ltpdwrrlms akvwgdaavq iffalspawg glitlssynk fnnncykdsl ivavsnigts
      361 ffagfvifsv igflaheldv pvasvvdqga glafivypev varlpvapvw sllffvmlls
      421 lgldsqfalm etvttailda fpalrnykfw vvfaaavfgy aggiifttna gmywlqlmdk
      481 yaanwsvlli aiaecilvaw iygadrfldd vqqmigprsr lwrffwtwmw kvitpatlff
      541 ilffnwveye plkyggyvyp rwadivgwvv gmipilvivf vainqlrdpr rrsayddddd
      601 ddddldgrps sqrsgkgktk ksknksvwwr akmllqptsd wgpaarnslt psrtlthtps
      661 pgppggydsv rdtivlvngq pmmvqpstts gtaqklpgps ilknssktdl itsqqv
//
LOCUS       XP_034193860             227 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane reductase CYB561D2 [Osmia lignaria lignaria].
ACCESSION   XP_034193860
VERSION     XP_034193860.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337969.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..227
                     /product="transmembrane reductase CYB561D2"
                     /calculated_mol_wt=24597
     Region          27..206
                     /region_name="Cyt_b561_CYB561D2_like"
                     /note="Eukaryotic cytochrome b(561), including the
                     CYB561D2 gene product; cd08761"
                     /db_xref="CDD:176491"
     Site            order(47,85,119,159)
                     /site_type="other"
                     /note="putative heme binding sites [chemical binding]"
                     /db_xref="CDD:176491"
     CDS             1..227
                     /gene="LOC117610491"
                     /coded_by="XM_034337969.2:189..872"
                     /db_xref="GeneID:117610491"
ORIGIN      
        1 mtdqpggkgp psaimlafsa lthfllvapv iyilvlafgh ysffawhpic msvgvgllit
       61 eavfsisgda yvsyklsrmn rvtmhwilht vglalvligl iiivvnkinn nkhhfatphs
      121 vlglvtiiis flvaafgivt nnskwlyprf rpvllkvlha fggivvtill latlitgtyt
      181 lwwpgsdtgr slvfssffia gffillkpil gavsrskvil apppttt
//
LOCUS       XP_034193871             227 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane reductase CYB561D2 [Osmia lignaria lignaria].
ACCESSION   XP_034193871
VERSION     XP_034193871.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..227
                     /product="transmembrane reductase CYB561D2"
                     /calculated_mol_wt=24597
     Region          27..206
                     /region_name="Cyt_b561_CYB561D2_like"
                     /note="Eukaryotic cytochrome b(561), including the
                     CYB561D2 gene product; cd08761"
                     /db_xref="CDD:176491"
     Site            order(47,85,119,159)
                     /site_type="other"
                     /note="putative heme binding sites [chemical binding]"
                     /db_xref="CDD:176491"
     CDS             1..227
                     /gene="LOC117610491"
                     /coded_by="XM_034337980.2:191..874"
                     /db_xref="GeneID:117610491"
ORIGIN      
        1 mtdqpggkgp psaimlafsa lthfllvapv iyilvlafgh ysffawhpic msvgvgllit
       61 eavfsisgda yvsyklsrmn rvtmhwilht vglalvligl iiivvnkinn nkhhfatphs
      121 vlglvtiiis flvaafgivt nnskwlyprf rpvllkvlha fggivvtill latlitgtyt
      181 lwwpgsdtgr slvfssffia gffillkpil gavsrskvil apppttt
//
LOCUS       XP_034194819             669 aa            linear   INV 03-SEP-2025
DEFINITION  inactive serine protease scarface [Osmia lignaria lignaria].
ACCESSION   XP_034194819
VERSION     XP_034194819.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338928.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194819.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..669
                     /product="inactive serine protease scarface"
                     /calculated_mol_wt=73321
     Region          212..277
                     /region_name="CLIP_SPH_Scar"
                     /note="Clip-domain serine protease homolog Scarface;
                     pfam18399"
                     /db_xref="CDD:465747"
     Region          389..625
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            order(427,480,580)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(574,600,602)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..669
                     /gene="scaf"
                     /coded_by="XM_034338928.2:141..2150"
                     /db_xref="GeneID:117611035"
ORIGIN      
        1 mriykpyiys tdrkkmlfga livvtsiwtt ngypstpptd ivlqdsaqqe vifqspeqqr
       61 arfqtsvqev eqepfnddff ayddskstsf sgqgadftis cvgvnkicvn khdcidgyih
      121 vansvaysls nkkgqcrvqt evccivaeey qgstvktvpl eqelssgtni gikvsppfgp
      181 sqeesggiss sqstegiaiq nafavavpvq vgcaaallcv eekfctadgt ispepvtlsg
      241 rqlfnrvpls scknpengii gkccrdpnyv dpwptgnlpa nysggfdeqg fptflniakv
      301 kanktptktp tktptkgetk sprpqipvpv eqkpepvllp ensvvsseil lppienvped
      361 khdenkvnlg qpqqicgvrn qvpqklkese tafaeipwqa mvlhaeerki lcsgalvstq
      421 dvltaancvd sllpndvsik lgewklgyev eqeeplpyki mnvsyinvhp gytrglgehd
      481 lailhleqpt afdyhinplc ipntkpplqq nrpcistgwg ksilqahyag aimhavsmni
      541 lpqeyckeqi ansdlrvntv dgsicttpke qqnnvcetdv ggplacenen glyelagiys
      601 qdtgcqatnq vaifaplddk wiketmfksi psetitiqre tstqykglli sndyrestls
      661 tdnqylppn
//
LOCUS       XP_034195460             557 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195460
VERSION     XP_034195460.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339569.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195460.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="BTB/POZ domain-containing protein 1 isoform X1"
                     /calculated_mol_wt=61475
     Region          127..253
                     /region_name="BTB_POZ_BTBD1_2"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing proteins, BTBD1 and BTBD2; cd18281"
                     /db_xref="CDD:349590"
     Region          249..343
                     /region_name="BACK_BTBD1_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing proteins, BTBD1 and BTBD2;
                     cd18487"
                     /db_xref="CDD:350562"
     Region          407..556
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     CDS             1..557
                     /gene="LOC117611584"
                     /coded_by="XM_034339569.2:690..2363"
                     /db_xref="GeneID:117611584"
ORIGIN      
        1 mssesslndv snvvsrlhtl rlghedndes qqnvnlaapg dsysepvcst qpignvtlsr
       61 trralnfvcd edqptsstar sgessssngd nrpptnaana lhlerrpqns ialenastns
      121 qatvaynwqg tkatmreriv flfnneilsd vsfivgrgaq kqripahklv lssgsavfda
      181 mfngtlatas seievpdvep aaflavllfl ytdeiqidpe tvmttlytak kyavaalekh
      241 cvdylknnlt sdnafllltq arlfdepqla avcldtidrf ttealnadgf tdididtlki
      301 vlerdtlrvr eskifqavvr wseaectrhq lpvapenqrr vlggafslvr fplmskeeft
      361 agpaqsgllt nsevlslfsy filnpkpmie fqtmprccmt gkeqtvcrfq htksrwgyng
      421 tsdrirfsvd rrifligygv ygsmhgpaiy evlielihta sgkviatnat sfscdgskyt
      481 yrlmfkelae ilpntiytas atfkgphshy gtkglktimv dsdsgngkvk fefnieagdn
      541 ngtsidegqi pelifyt
//
LOCUS       XP_076546534             556 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain-containing protein 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546534
VERSION     XP_076546534.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690419.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..556
                     /product="BTB/POZ domain-containing protein 1 isoform X2"
                     /calculated_mol_wt=61332
     Region          126..252
                     /region_name="BTB_POZ_BTBD1_2"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing proteins, BTBD1 and BTBD2; cd18281"
                     /db_xref="CDD:349590"
     Region          248..342
                     /region_name="BACK_BTBD1_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing proteins, BTBD1 and BTBD2;
                     cd18487"
                     /db_xref="CDD:350562"
     Region          406..555
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     CDS             1..556
                     /gene="LOC117611584"
                     /coded_by="XM_076690419.1:699..2369"
                     /db_xref="GeneID:117611584"
ORIGIN      
        1 mssesslndv snvvsrlhtl rlghedndes qqnvnlaapg dsysepvcst qpignvtlsr
       61 trralnfvcd edqptsstar sgessssngd kpptnaanal hlerrpqnsi alenastnsq
      121 atvaynwqgt katmrerivf lfnneilsdv sfivgrgaqk qripahklvl ssgsavfdam
      181 fngtlatass eievpdvepa aflavllfly tdeiqidpet vmttlytakk yavaalekhc
      241 vdylknnlts dnafllltqa rlfdepqlaa vcldtidrft tealnadgft dididtlkiv
      301 lerdtlrvre skifqavvrw seaectrhql pvapenqrrv lggafslvrf plmskeefta
      361 gpaqsglltn sevlslfsyf ilnpkpmief qtmprccmtg keqtvcrfqh tksrwgyngt
      421 sdrirfsvdr rifligygvy gsmhgpaiye vlielihtas gkviatnats fscdgskyty
      481 rlmfkelaei lpntiytasa tfkgphshyg tkglktimvd sdsgngkvkf efnieagdnn
      541 gtsidegqip elifyt
//
LOCUS       XP_034194827             242 aa            linear   INV 03-SEP-2025
DEFINITION  dnaJ homolog subfamily C member 5 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194827
VERSION     XP_034194827.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338936.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="dnaJ homolog subfamily C member 5 isoform X1"
                     /calculated_mol_wt=26734
     Region          12..74
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(40..42,51,54..55,58..59)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..242
                     /gene="LOC117611045"
                     /coded_by="XM_034338936.2:196..924"
                     /db_xref="GeneID:117611045"
ORIGIN      
        1 mdkrkmstag dslyqileip ktatpeeikk tyrklalkyh pdknpnnpea tekfkeinra
       61 hsiltdlskr niydnygslg lyiaeqfged nvnaycvncr wfnalfffca litacycccc
      121 cccccnfccg kckptppeds gayhnlqrdq npdvdvvtnq prapakeees dedaiiaqpq
      181 ggppnnsnnq qpifampppp ttadentnln sgaerviytt aastnpfaav nvgttdtstt
      241 kw
//
LOCUS       XP_034194831             223 aa            linear   INV 03-SEP-2025
DEFINITION  dnaJ homolog subfamily C member 5 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194831
VERSION     XP_034194831.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338940.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="dnaJ homolog subfamily C member 5 isoform X2"
                     /calculated_mol_wt=24632
     Region          12..74
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(40..42,51,54..55,58..59)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     CDS             1..223
                     /gene="LOC117611045"
                     /coded_by="XM_034338940.2:199..870"
                     /db_xref="GeneID:117611045"
ORIGIN      
        1 mdkrkmstag dslyqileip ktatpeeikk tyrklalkyh pdknpnnpea tekfkeinra
       61 hsiltdlskr niydnygslg lyiaeqfged nvnaycvncr wfnalfffca litacycccc
      121 cccccnfccg kckptppeds gayhnlqeee sdedaiiaqp qggppnnsnn qqpifamppp
      181 pttadentnl nsgaerviyt taastnpfaa vnvgttdtst tkw
//
LOCUS       XP_034194071             764 aa            linear   INV 03-SEP-2025
DEFINITION  conserved oligomeric Golgi complex subunit 5 four way stop isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194071
VERSION     XP_034194071.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338180.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..764
                     /product="conserved oligomeric Golgi complex subunit 5
                     four way stop isoform X1"
                     /calculated_mol_wt=85317
     Region          23..136
                     /region_name="COG5"
                     /note="Golgi transport complex subunit 5; pfam10392"
                     /db_xref="CDD:192566"
     CDS             1..764
                     /gene="fws"
                     /coded_by="XM_034338180.2:215..2509"
                     /db_xref="GeneID:117610593"
ORIGIN      
        1 mneitnweei etddflkqfl dgkskkadvn qvlsvakqin klgqaieiln selqkqvlan
       61 hedllsqatw veklegvlsi mqshvqslls averlrgkiv dpfnrietqt vvlarlhets
      121 dllrrvarmq hlskrlysqm tnnvqgpdii kaanslhele qlmmdtdlng ldviaddqqa
      181 vkahrmtvqr lathtltqgl qtmdrgkist avqvfenlgi vngaidnimd salseiekia
      241 tesldvtlas nhdfgkrgap graaipspgs sgnlrtriwd nlerlfqdtl ythclqiell
      301 qrillehhik gldelsqkfw drvncllskv lieraqgssf vkqalegeyp kflrifldls
      361 krlkersrsi giyhidrnvl lpfenaylsr svsrlldpvh tmfsgeglpt qdeidslirt
      421 vtnelsvslv ddglsivvar nvgkairlfc lkceqgllvg geasqvidsp tavqqtnvll
      481 anllyylssq inrvianlst glpsegstvi tvalketdkl tknilsplla sisdaiesii
      541 ltmhddsefr dpssplgkei ncslymrelq gfilrsvntf lapyknqavv aeccktvasr
      601 cielflrhac llrpltdfgk akliadfnqi evavaplcrg gqlgsseqqq yrtlralkal
      661 ipfnpdeivh kvlegdgdcg iprslvlshl fstappelvs phqnanwsvg rlsqwmdnhp
      721 kerdrisffs galeryqltv rqqnlptfhp lfplmkkltn sngh
//
LOCUS       XP_034194063             759 aa            linear   INV 03-SEP-2025
DEFINITION  conserved oligomeric Golgi complex subunit 5 four way stop isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194063
VERSION     XP_034194063.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194063.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..759
                     /product="conserved oligomeric Golgi complex subunit 5
                     four way stop isoform X2"
                     /calculated_mol_wt=84699
     Region          18..131
                     /region_name="COG5"
                     /note="Golgi transport complex subunit 5; pfam10392"
                     /db_xref="CDD:192566"
     CDS             1..759
                     /gene="fws"
                     /coded_by="XM_034338172.2:22..2301"
                     /db_xref="GeneID:117610593"
ORIGIN      
        1 mtlskytynf lkqfldgksk kadvnqvlsv akqinklgqa ieilnselqk qvlanhedll
       61 sqatwvekle gvlsimqshv qsllsaverl rgkivdpfnr ietqtvvlar lhetsdllrr
      121 varmqhlskr lysqmtnnvq gpdiikaans lheleqlmmd tdlngldvia ddqqavkahr
      181 mtvqrlatht ltqglqtmdr gkistavqvf enlgivngai dnimdsalse iekiatesld
      241 vtlasnhdfg krgapgraai pspgssgnlr triwdnlerl fqdtlythcl qiellqrill
      301 ehhikgldel sqkfwdrvnc llskvliera qgssfvkqal egeypkflri fldlskrlke
      361 rsrsigiyhi drnvllpfen aylsrsvsrl ldpvhtmfsg eglptqdeid slirtvtnel
      421 svslvddgls ivvarnvgka irlfclkceq gllvggeasq vidsptavqq tnvllanlly
      481 ylssqinrvi anlstglpse gstvitvalk etdkltknil spllasisda iesiiltmhd
      541 dsefrdpssp lgkeincsly mrelqgfilr svntflapyk nqavvaecck tvasrcielf
      601 lrhacllrpl tdfgkaklia dfnqievava plcrggqlgs seqqqyrtlr alkalipfnp
      661 deivhkvleg dgdcgiprsl vlshlfstap pelvsphqna nwsvgrlsqw mdnhpkerdr
      721 isffsgaler yqltvrqqnl ptfhplfplm kkltnsngh
//
LOCUS       XP_076546535             630 aa            linear   INV 03-SEP-2025
DEFINITION  conserved oligomeric Golgi complex subunit 5 four way stop isoform
            X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546535
VERSION     XP_076546535.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690420.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="conserved oligomeric Golgi complex subunit 5
                     four way stop isoform X3"
                     /calculated_mol_wt=70269
     Region          23..136
                     /region_name="COG5"
                     /note="Golgi transport complex subunit 5; pfam10392"
                     /db_xref="CDD:192566"
     CDS             1..630
                     /gene="fws"
                     /coded_by="XM_076690420.1:215..2107"
                     /db_xref="GeneID:117610593"
ORIGIN      
        1 mneitnweei etddflkqfl dgkskkadvn qvlsvakqin klgqaieiln selqkqvlan
       61 hedllsqatw veklegvlsi mqshvqslls averlrgkiv dpfnrietqt vvlarlhets
      121 dllrrvarmq hlskrlysqm tnnvqgpdii kaanslhele qlmmdtdlng ldviaddqqa
      181 vkahrmtvqr lathtltqgl qtmdrgkist avqvfenlgi vngaidnimd salseiekia
      241 tesldvtlas nhdfgkrgap graaipspgs sgnlrtriwd nlerlfqdtl ythclqiell
      301 qrillehhik gldelsqkfw drvncllskv lieraqgssf vkqalegeyp kflrifldls
      361 krlkersrsi giyhidrnvl lpfenaylsr svsrlldpvh tmfsgeglpt qdeidslirt
      421 vtnelsvslv ddglsivvar nvgkairlfc lkceqgllvg geasqvidsp tavqqtnvll
      481 anllyylssq inrvianlst glpsegstvi tvalketdkl tknilsplla sisdaiesii
      541 ltmhddsefr dpssplgkei ncslymrelq gfilrsvntf lapyknqavv aeccktvasr
      601 cielflrhac llrpltdfgk akliadfnqk
//
LOCUS       XP_034194091             568 aa            linear   INV 03-SEP-2025
DEFINITION  asparagine synthetase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194091
VERSION     XP_034194091.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338200.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194091.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..568
                     /product="asparagine synthetase isoform X1"
                     /calculated_mol_wt=64893
     Region          1..559
                     /region_name="asnB"
                     /note="asparagine synthetase B; Provisional; PRK09431"
                     /db_xref="CDD:236513"
     CDS             1..568
                     /gene="AsnS"
                     /coded_by="XM_034338200.2:208..1914"
                     /db_xref="GeneID:117610610"
ORIGIN      
        1 mcgiwalfgl dtqpsthiyq nfgkithrgp eafrlefdsr vkngylgfhr laivdslcgm
       61 qpmrlyeyph lfllcngeiy nhkeisqdhg ykrvtkcdve vilhlyadgg venvakmldg
      121 vfafclmdik trriliardp ygvrpifrfy skngqlavcs eskglmgitk qitsesvlep
      181 fppgyyeeye ilddgrtkll skvnyhkpdd kpsfkvyvpw nalsnldvhg nirklllaai
      241 kkrlmtdrei gcllsgglds sliaallakh akevnlpyki ksfaigmgds pdiiaarqva
      301 dyigtehhev ifsekdviei ldkliyqlet fdittirasi gmylvsryik hntkttvifs
      361 gegadelaqg yiyfrdapna ieahnesirl lkdiylydgl radrttsafs lelrvpfldl
      421 qftnyylsld vasrqpqggi ekyllrsafe gtnllpsnil wrhkeafsdg vtsikkslfq
      481 iihdvvdvrv sdddlekayl kyphctpktk eafyyrkvfe khyegrakhf vpyfwmprwv
      541 tgvsdpsarf iahyaadkkn ncikndkd
//
LOCUS       XP_034194100             568 aa            linear   INV 03-SEP-2025
DEFINITION  asparagine synthetase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194100
VERSION     XP_034194100.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338209.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194100.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..568
                     /product="asparagine synthetase isoform X1"
                     /calculated_mol_wt=64893
     Region          1..559
                     /region_name="asnB"
                     /note="asparagine synthetase B; Provisional; PRK09431"
                     /db_xref="CDD:236513"
     CDS             1..568
                     /gene="AsnS"
                     /coded_by="XM_034338209.2:88..1794"
                     /db_xref="GeneID:117610610"
ORIGIN      
        1 mcgiwalfgl dtqpsthiyq nfgkithrgp eafrlefdsr vkngylgfhr laivdslcgm
       61 qpmrlyeyph lfllcngeiy nhkeisqdhg ykrvtkcdve vilhlyadgg venvakmldg
      121 vfafclmdik trriliardp ygvrpifrfy skngqlavcs eskglmgitk qitsesvlep
      181 fppgyyeeye ilddgrtkll skvnyhkpdd kpsfkvyvpw nalsnldvhg nirklllaai
      241 kkrlmtdrei gcllsgglds sliaallakh akevnlpyki ksfaigmgds pdiiaarqva
      301 dyigtehhev ifsekdviei ldkliyqlet fdittirasi gmylvsryik hntkttvifs
      361 gegadelaqg yiyfrdapna ieahnesirl lkdiylydgl radrttsafs lelrvpfldl
      421 qftnyylsld vasrqpqggi ekyllrsafe gtnllpsnil wrhkeafsdg vtsikkslfq
      481 iihdvvdvrv sdddlekayl kyphctpktk eafyyrkvfe khyegrakhf vpyfwmprwv
      541 tgvsdpsarf iahyaadkkn ncikndkd
//
LOCUS       XP_034194117             509 aa            linear   INV 03-SEP-2025
DEFINITION  asparagine synthetase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194117
VERSION     XP_034194117.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338226.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194117.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..509
                     /product="asparagine synthetase isoform X2"
                     /calculated_mol_wt=58225
     Region          14..500
                     /region_name="asnB"
                     /note="asparagine synthetase B; Provisional; PRK09431"
                     /db_xref="CDD:236513"
     CDS             1..509
                     /gene="AsnS"
                     /coded_by="XM_034338226.2:197..1726"
                     /db_xref="GeneID:117610610"
ORIGIN      
        1 mqpmrlyeyp hlfllcngei ynhkeisqdh gykrvtkcdv evilhlyadg gvenvakmld
       61 gvfafclmdi ktrriliard pygvrpifrf yskngqlavc seskglmgit kqitsesvle
      121 pfppgyyeey eilddgrtkl lskvnyhkpd dkpsfkvyvp wnalsnldvh gnirklllaa
      181 ikkrlmtdre igcllsggld ssliaallak hakevnlpyk iksfaigmgd spdiiaarqv
      241 adyigtehhe vifsekdvie ildkliyqle tfdittiras igmylvsryi khntkttvif
      301 sgegadelaq gyiyfrdapn aieahnesir llkdiylydg lradrttsaf slelrvpfld
      361 lqftnyylsl dvasrqpqgg iekyllrsaf egtnllpsni lwrhkeafsd gvtsikkslf
      421 qiihdvvdvr vsdddlekay lkyphctpkt keafyyrkvf ekhyegrakh fvpyfwmprw
      481 vtgvsdpsar fiahyaadkk nncikndkd
//
LOCUS       XP_034194126             509 aa            linear   INV 03-SEP-2025
DEFINITION  asparagine synthetase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194126
VERSION     XP_034194126.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194126.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..509
                     /product="asparagine synthetase isoform X2"
                     /calculated_mol_wt=58225
     Region          14..500
                     /region_name="asnB"
                     /note="asparagine synthetase B; Provisional; PRK09431"
                     /db_xref="CDD:236513"
     CDS             1..509
                     /gene="AsnS"
                     /coded_by="XM_034338235.2:166..1695"
                     /db_xref="GeneID:117610610"
ORIGIN      
        1 mqpmrlyeyp hlfllcngei ynhkeisqdh gykrvtkcdv evilhlyadg gvenvakmld
       61 gvfafclmdi ktrriliard pygvrpifrf yskngqlavc seskglmgit kqitsesvle
      121 pfppgyyeey eilddgrtkl lskvnyhkpd dkpsfkvyvp wnalsnldvh gnirklllaa
      181 ikkrlmtdre igcllsggld ssliaallak hakevnlpyk iksfaigmgd spdiiaarqv
      241 adyigtehhe vifsekdvie ildkliyqle tfdittiras igmylvsryi khntkttvif
      301 sgegadelaq gyiyfrdapn aieahnesir llkdiylydg lradrttsaf slelrvpfld
      361 lqftnyylsl dvasrqpqgg iekyllrsaf egtnllpsni lwrhkeafsd gvtsikkslf
      421 qiihdvvdvr vsdddlekay lkyphctpkt keafyyrkvf ekhyegrakh fvpyfwmprw
      481 vtgvsdpsar fiahyaadkk nncikndkd
//
LOCUS       XP_034194141             430 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610628 [Osmia lignaria lignaria].
ACCESSION   XP_034194141
VERSION     XP_034194141.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338250.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194141.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="uncharacterized protein LOC117610628"
                     /calculated_mol_wt=48922
     Region          <33..197
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          41..70
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(42,46..47,50..52,54..55,59,62,70,72,74,78..79,
                     84..86,88..89,97,100,109,111,113,150..151,154..156,
                     158..159,163,166,175)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          72..109
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          111..175
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          116..142
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          236..289
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cd09487"
                     /db_xref="CDD:188886"
     CDS             1..430
                     /gene="LOC117610628"
                     /coded_by="XM_034338250.2:285..1577"
                     /db_xref="GeneID:117610628"
ORIGIN      
        1 meidekqmfe snminacmmg hfqvihkyle lynindvlct gwtpllyaas gaqaevidyl
       61 ikngadvnkh kdgytplmil cnsskntvet rltclklltk aganvnasnk qkqtplmfas
      121 msqepefvte likhvenida ydnrkqtalm yaiiankpei vkilvkntan valtdindlt
      181 akdiasikgy dkilsllhfn eeeeetipty eiakmynwmd mfplltnisn ktidfdvfti
      241 lhgmglekys yifqgtslka flklteqdlc rlgieinahr iqfiehlhkf hrkkwsiqsi
      301 gaidkslpyt lydgtvslgt vakqvaiigt sfehikndll kandnnihlt keqmsnyeeg
      361 ivktqktlcm lkkeleqmga lskkirkddn igtpatyicp kkhrinwsif lgitltvgiy
      421 fskrvyiqty
//
LOCUS       XP_034194150             417 aa            linear   INV 03-SEP-2025
DEFINITION  AP-3 complex subunit carmine isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194150
VERSION     XP_034194150.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338259.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..417
                     /product="AP-3 complex subunit carmine isoform X1"
                     /calculated_mol_wt=47100
     Region          3..141
                     /region_name="AP3_Mu_N"
                     /note="AP-3 complex subunit mu N-terminal domain; cd14837"
                     /db_xref="CDD:341441"
     Site            order(18,21,46..47,55,57,59,71..73,75..77,80,84,110..111,
                     114..115,117..119,121..124,133,135..136)
                     /site_type="other"
                     /note="putative AP-3 beta interface [polypeptide binding]"
                     /db_xref="CDD:341441"
     Region          165..416
                     /region_name="AP_MHD_Cterm"
                     /note="C-terminal domain of adaptor protein (AP) complexes
                     medium mu subunits and its homologs (MHD); cl10970"
                     /db_xref="CDD:472082"
     CDS             1..417
                     /gene="carmine"
                     /coded_by="XM_034338259.2:204..1457"
                     /db_xref="GeneID:117610637"
ORIGIN      
        1 mihslfiins sgdvfmekhw ksavarslcd yffdqqrrvl spedtppvia tphhylisiy
       61 rcnmffvavc mtevpplfvi eflhrvvdtf edyfsectet iikenyvvvy elldemldng
      121 fplatesnil kelikppnil rtiantvtgk snvsailpsg qlsnvpwrrt gvkytnneay
      181 fdvveevdai idrtgatvfa eiqgyidcci klsgmpdltl sfmnprlfdd vsfhpcvrfk
      241 rweserilsf ippdgnfrll syhigsqsiv aipiyvrhni slkepgggrl ditvgpkqti
      301 grtvenvtle ipmpkivlnc tltpnqgkys fdpvskillw digridvskl pnlrgsitiq
      361 ssasvlesnp ainvhftinq lavsglkvnr ldmygekykp fkgvkyitka gkfqirm
//
LOCUS       XP_034194160             402 aa            linear   INV 03-SEP-2025
DEFINITION  AP-3 complex subunit carmine isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194160
VERSION     XP_034194160.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338269.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="AP-3 complex subunit carmine isoform X2"
                     /calculated_mol_wt=45438
     Region          1..126
                     /region_name="AP3_Mu_N"
                     /note="AP-3 complex subunit mu N-terminal domain; cd14837"
                     /db_xref="CDD:341441"
     Site            order(3,6,31..32,40,42,44,56..58,60..62,65,69,95..96,
                     99..100,102..104,106..109,118,120..121)
                     /site_type="other"
                     /note="putative AP-3 beta interface [polypeptide binding]"
                     /db_xref="CDD:341441"
     Region          150..401
                     /region_name="AP_MHD_Cterm"
                     /note="C-terminal domain of adaptor protein (AP) complexes
                     medium mu subunits and its homologs (MHD); cl10970"
                     /db_xref="CDD:472082"
     CDS             1..402
                     /gene="carmine"
                     /coded_by="XM_034338269.2:112..1320"
                     /db_xref="GeneID:117610637"
ORIGIN      
        1 mekhwksava rslcdyffdq qrrvlspedt ppviatphhy lisiyrcnmf fvavcmtevp
       61 plfvieflhr vvdtfedyfs ectetiiken yvvvyellde mldngfplat esnilkelik
      121 ppnilrtian tvtgksnvsa ilpsgqlsnv pwrrtgvkyt nneayfdvve evdaiidrtg
      181 atvfaeiqgy idcciklsgm pdltlsfmnp rlfddvsfhp cvrfkrwese rilsfippdg
      241 nfrllsyhig sqsivaipiy vrhnislkep gggrlditvg pkqtigrtve nvtleipmpk
      301 ivlnctltpn qgkysfdpvs killwdigri dvsklpnlrg sitiqssasv lesnpainvh
      361 ftinqlavsg lkvnrldmyg ekykpfkgvk yitkagkfqi rm
//
LOCUS       XP_034194162             320 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional adapter 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194162
VERSION     XP_034194162.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338271.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194162.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="transcriptional adapter 1 isoform X1"
                     /calculated_mol_wt=36378
     Region          14..>66
                     /region_name="SAGA-Tad1"
                     /note="Transcriptional regulator of RNA polII, SAGA,
                     subunit; pfam12767"
                     /db_xref="CDD:463692"
     Region          139..222
                     /region_name="HFD_TADA1"
                     /note="histone-fold domain found in transcriptional
                     adapter 1 (TADA1) and similar proteins; cd22934"
                     /db_xref="CDD:467058"
     CDS             1..320
                     /gene="LOC117610645"
                     /coded_by="XM_034338271.2:92..1054"
                     /db_xref="GeneID:117610645"
ORIGIN      
        1 mttskelnla rkslvaslge nakvyfekmk lwfqmkttke efdcearnim tedqvhlhne
       61 fllclfnkvr glaaaapirk idrekaikek rlrlkrkykt dksnfepadi yvevlsqtss
      121 pvgdepigan rssaqelvlp drtfvlarlm laawennmdg aeentahivi aatqiflkni
      181 ltaiftrrkg yavrdgsfiy nigepvpssw krnsihisrp sdynvtdmvd pygqvptikp
      241 nfeeaeqata fayacssqti ppplkpvnta dlqytlkiyk nlvnshtiya tnmerlftya
      301 thqtwedlea kkllnyqpat
//
LOCUS       XP_034194167             292 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional adapter 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194167
VERSION     XP_034194167.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338276.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194167.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="transcriptional adapter 1 isoform X2"
                     /calculated_mol_wt=33267
     Region          111..194
                     /region_name="HFD_TADA1"
                     /note="histone-fold domain found in transcriptional
                     adapter 1 (TADA1) and similar proteins; cd22934"
                     /db_xref="CDD:467058"
     CDS             1..292
                     /gene="LOC117610645"
                     /coded_by="XM_034338276.2:500..1378"
                     /db_xref="GeneID:117610645"
ORIGIN      
        1 mklwfqmktt keefdcearn imtedqvhlh nefllclfnk vrglaaaapi rkidrekaik
       61 ekrlrlkrky ktdksnfepa diyvevlsqt sspvgdepig anrssaqelv lpdrtfvlar
      121 lmlaawennm dgaeentahi viaatqiflk niltaiftrr kgyavrdgsf iynigepvps
      181 swkrnsihis rpsdynvtdm vdpygqvpti kpnfeeaeqa tafayacssq tippplkpvn
      241 tadlqytlki yknlvnshti yatnmerlft yathqtwedl eakkllnyqp at
//
LOCUS       XP_034193884             485 aa            linear   INV 03-SEP-2025
DEFINITION  chitin deacetylase Cda4 [Osmia lignaria lignaria].
ACCESSION   XP_034193884
VERSION     XP_034193884.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="chitin deacetylase Cda4"
                     /calculated_mol_wt=55434
     Region          40..90
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     Region          140..406
                     /region_name="CE4_CDA_like_1"
                     /note="Putative catalytic domain of chitin
                     deacetylase-like proteins with additional chitin-binding
                     peritrophin-A domain (ChBD) and/or a low-density
                     lipoprotein receptor class A domain (LDLa); cd10974"
                     /db_xref="CDD:200596"
     Site            order(146..149,204..209,246,248,272,363,365)
                     /site_type="other"
                     /note="NodB motif"
                     /db_xref="CDD:200596"
     Site            order(148..149,204,208,248..250,365)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:200596"
     CDS             1..485
                     /gene="Cda4"
                     /coded_by="XM_034337993.2:90..1547"
                     /db_xref="GeneID:117610497"
ORIGIN      
        1 mmtlrrsrfl lcaalivaas gqkngkkdee kkdeefkcpe gqgngnfadp atcrrfyqcv
       61 dgypylnrcp sglhfddisk fctfknearc gpiattpapv tepptdlaek cdtsecqlpy
      121 cfcsrdgtii pgglhpedtp qmilmtfdga inhnnfdhyq kifntdrlnp nncplkgtff
      181 isheycnynm vqsiahdghe iatetislqk gledkgyeew vgemigmrei lksfsnisis
      241 eivgmrapyl kpgrntqykv ledfgyiyds sigisplkvp iwpytldyki pheckagtcp
      301 tksfpgvwel plnahyvesy egghcpyldq cvlhnhdpee vfewlqedfn ryyeqnrapy
      361 mmpfhtnwfq ikelerglsk fldwavtlpd vyfvtatqal twitdpkptk slnnfegwsc
      421 krkenipgpp cnnankcald fkppesnftt tryletcrec pnkypwlgds kgtglyndny
      481 nperk
//
LOCUS       XP_034193898             465 aa            linear   INV 03-SEP-2025
DEFINITION  tetratricopeptide repeat protein 8 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193898
VERSION     XP_034193898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338007.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..465
                     /product="tetratricopeptide repeat protein 8 isoform X1"
                     /calculated_mol_wt=52652
     Region          26..162
                     /region_name="TTC8_N"
                     /note="N-terminal domain of tetratricopeptide repeat
                     domain 8; cd21341"
                     /db_xref="CDD:411061"
     Region          213..443
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          213..236
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          241..269
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          275..303
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          310..337
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(344,347..348,351..352,354,380,383..384,387..388,
                     390..391,414,417..418,421..422,425)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          344..371
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          378..408
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          413..439
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..465
                     /gene="BBS8"
                     /coded_by="XM_034338007.2:40..1437"
                     /db_xref="GeneID:117610504"
ORIGIN      
        1 mmeffkalsl fdrgryeeca tictnllrks pldqavwvlk mrtltlqvyv ddiegeeegi
       61 aetlldnyti ssmprpgtsl kqpgtsyagq ylrpktqsgr pvtgvvrpat qsatsqsleq
      121 tlrtprtamt arpitasssr nvrlgtasml tepggpfiql srlniskyas qasiakplfe
      181 yiyyhehdvr yaldlavqat qvcqykdwww kvqlgkcyys lglvrdaeqq fksalrdckt
      241 ietilrlvrv yikldqplaa idackkgleh fpndvnilte mgrifdglnn msmslkyyki
      301 ivqedashte aiasigmhhf ysdqpelalr yyrrllqmgv yntelfnnlg lccfyaqqyd
      361 hviscferal slstdeniad vwynishial sigdtimaee clkltivsdn rhalaynnlg
      421 viqirngnit aaktyfhaaa nianfiyeph fnsaylayev vqivq
//
LOCUS       XP_034193906             462 aa            linear   INV 03-SEP-2025
DEFINITION  tetratricopeptide repeat protein 8 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193906
VERSION     XP_034193906.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338015.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="tetratricopeptide repeat protein 8 isoform X2"
                     /calculated_mol_wt=52234
     Region          26..162
                     /region_name="TTC8_N"
                     /note="N-terminal domain of tetratricopeptide repeat
                     domain 8; cd21341"
                     /db_xref="CDD:411061"
     Region          210..440
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          210..233
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          238..266
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          272..300
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          307..334
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(341,344..345,348..349,351,377,380..381,384..385,
                     387..388,411,414..415,418..419,422)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          341..368
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          375..405
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          410..436
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..462
                     /gene="BBS8"
                     /coded_by="XM_034338015.2:40..1428"
                     /db_xref="GeneID:117610504"
ORIGIN      
        1 mmeffkalsl fdrgryeeca tictnllrks pldqavwvlk mrtltlqvyv ddiegeeegi
       61 aetlldnyti ssmprpgtsl kqpgtsyagq ylrpktqsgr pvtgvvrpat qsatsqsleq
      121 tlrtprtamt arpitasssr nvrlgtasml tepggpfiql srlniskyas qasiakplfe
      181 yiyyhehdal dlavqatqvc qykdwwwkvq lgkcyyslgl vrdaeqqfks alrdcktiet
      241 ilrlvrvyik ldqplaaida ckkglehfpn dvniltemgr ifdglnnmsm slkyykiivq
      301 edashteaia sigmhhfysd qpelalryyr rllqmgvynt elfnnlglcc fyaqqydhvi
      361 scferalsls tdeniadvwy nishialsig dtimaeeclk ltivsdnrha laynnlgviq
      421 irngnitaak tyfhaaania nfiyephfns aylayevvqi vq
//
LOCUS       XP_034193922             237 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 134 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193922
VERSION     XP_034193922.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338031.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="coiled-coil domain-containing protein 134"
                     /calculated_mol_wt=27535
     Region          39..203
                     /region_name="ERK-JNK_inhib"
                     /note="ERK and JNK pathways, inhibitor; pfam15002"
                     /db_xref="CDD:464435"
     CDS             1..237
                     /gene="LOC117610514"
                     /coded_by="XM_034338031.2:160..873"
                     /db_xref="GeneID:117610514"
ORIGIN      
        1 mkkmprfviy ivvvstltrv thvqqvlsdi ennppisekp qngnpseiki yeelfrksfv
       61 hqrkehteai krlrnidnye rlykmitilg tkmidiieas kqliestdfn pddrslpknv
      121 tiqsaisttl entalfadil lhfphmthri lktqkewnpv inwslhftnr tkhlldaetl
      181 tvvhlaaqel niierepgyv npywqstgsq gedeekkkkk kskkkaqrvp ritktdl
//
LOCUS       XP_034193939             237 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 134 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193939
VERSION     XP_034193939.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338048.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="coiled-coil domain-containing protein 134"
                     /calculated_mol_wt=27535
     Region          39..203
                     /region_name="ERK-JNK_inhib"
                     /note="ERK and JNK pathways, inhibitor; pfam15002"
                     /db_xref="CDD:464435"
     CDS             1..237
                     /gene="LOC117610514"
                     /coded_by="XM_034338048.2:160..873"
                     /db_xref="GeneID:117610514"
ORIGIN      
        1 mkkmprfviy ivvvstltrv thvqqvlsdi ennppisekp qngnpseiki yeelfrksfv
       61 hqrkehteai krlrnidnye rlykmitilg tkmidiieas kqliestdfn pddrslpknv
      121 tiqsaisttl entalfadil lhfphmthri lktqkewnpv inwslhftnr tkhlldaetl
      181 tvvhlaaqel niierepgyv npywqstgsq gedeekkkkk kskkkaqrvp ritktdl
//
LOCUS       XP_034193929             237 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 134 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193929
VERSION     XP_034193929.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338038.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="coiled-coil domain-containing protein 134"
                     /calculated_mol_wt=27535
     Region          39..203
                     /region_name="ERK-JNK_inhib"
                     /note="ERK and JNK pathways, inhibitor; pfam15002"
                     /db_xref="CDD:464435"
     CDS             1..237
                     /gene="LOC117610514"
                     /coded_by="XM_034338038.2:160..873"
                     /db_xref="GeneID:117610514"
ORIGIN      
        1 mkkmprfviy ivvvstltrv thvqqvlsdi ennppisekp qngnpseiki yeelfrksfv
       61 hqrkehteai krlrnidnye rlykmitilg tkmidiieas kqliestdfn pddrslpknv
      121 tiqsaisttl entalfadil lhfphmthri lktqkewnpv inwslhftnr tkhlldaetl
      181 tvvhlaaqel niierepgyv npywqstgsq gedeekkkkk kskkkaqrvp ritktdl
//
LOCUS       XP_034193953             228 aa            linear   INV 03-SEP-2025
DEFINITION  protein YIPF6 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193953
VERSION     XP_034193953.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338062.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="protein YIPF6 isoform X1"
                     /calculated_mol_wt=25459
     Region          <45..224
                     /region_name="YIP1"
                     /note="Rab GTPase interacting factor, Golgi membrane
                     protein [Intracellular trafficking and secretion];
                     cl46908"
                     /db_xref="CDD:481248"
     CDS             1..228
                     /gene="LOC117610528"
                     /coded_by="XM_034338062.2:565..1251"
                     /db_xref="GeneID:117610528"
ORIGIN      
        1 maatedakld fkvtmeyads qnvegemtvg akqksnlgep dfntldepik dtilrdvcav
       61 gkkfyhvlyp kekksllkew dlwgplvlct fmamtlqgss drandpndgg peftevfviv
      121 wigsmivtln skllggnisf fqsicvlgyc llptaialil criilmveqt tflfslrfii
      181 smigfiwaty asmvflgdsq pagrkalavy piflfyfvis wlvisrtr
//
LOCUS       XP_034193962             228 aa            linear   INV 03-SEP-2025
DEFINITION  protein YIPF6 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193962
VERSION     XP_034193962.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338071.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..228
                     /product="protein YIPF6 isoform X1"
                     /calculated_mol_wt=25459
     Region          <45..224
                     /region_name="YIP1"
                     /note="Rab GTPase interacting factor, Golgi membrane
                     protein [Intracellular trafficking and secretion];
                     cl46908"
                     /db_xref="CDD:481248"
     CDS             1..228
                     /gene="LOC117610528"
                     /coded_by="XM_034338071.2:456..1142"
                     /db_xref="GeneID:117610528"
ORIGIN      
        1 maatedakld fkvtmeyads qnvegemtvg akqksnlgep dfntldepik dtilrdvcav
       61 gkkfyhvlyp kekksllkew dlwgplvlct fmamtlqgss drandpndgg peftevfviv
      121 wigsmivtln skllggnisf fqsicvlgyc llptaialil criilmveqt tflfslrfii
      181 smigfiwaty asmvflgdsq pagrkalavy piflfyfvis wlvisrtr
//
LOCUS       XP_034193975             217 aa            linear   INV 03-SEP-2025
DEFINITION  protein YIPF6 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193975
VERSION     XP_034193975.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338084.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="protein YIPF6 isoform X2"
                     /calculated_mol_wt=24269
     Region          <34..213
                     /region_name="YIP1"
                     /note="Rab GTPase interacting factor, Golgi membrane
                     protein [Intracellular trafficking and secretion];
                     cl46908"
                     /db_xref="CDD:481248"
     CDS             1..217
                     /gene="LOC117610528"
                     /coded_by="XM_034338084.2:19..672"
                     /db_xref="GeneID:117610528"
ORIGIN      
        1 mvtmeyadsq nvegemtvga kqksnlgepd fntldepikd tilrdvcavg kkfyhvlypk
       61 ekksllkewd lwgplvlctf mamtlqgssd randpndggp eftevfvivw igsmivtlns
      121 kllggnisff qsicvlgycl lptaialilc riilmveqtt flfslrfiis migfiwatya
      181 smvflgdsqp agrkalavyp iflfyfvisw lvisrtr
//
LOCUS       XP_034193982             214 aa            linear   INV 03-SEP-2025
DEFINITION  protein YIPF6 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193982
VERSION     XP_034193982.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338091.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..214
                     /product="protein YIPF6 isoform X3"
                     /calculated_mol_wt=23937
     Region          <31..210
                     /region_name="YIP1"
                     /note="Rab GTPase interacting factor, Golgi membrane
                     protein [Intracellular trafficking and secretion];
                     cl46908"
                     /db_xref="CDD:481248"
     CDS             1..214
                     /gene="LOC117610528"
                     /coded_by="XM_034338091.2:368..1012"
                     /db_xref="GeneID:117610528"
ORIGIN      
        1 meyadsqnve gemtvgakqk snlgepdfnt ldepikdtil rdvcavgkkf yhvlypkekk
       61 sllkewdlwg plvlctfmam tlqgssdran dpndggpeft evfvivwigs mivtlnskll
      121 ggnisffqsi cvlgycllpt aialilcrii lmveqttflf slrfiismig fiwatyasmv
      181 flgdsqpagr kalavypifl fyfviswlvi srtr
//
LOCUS       XP_034193988             214 aa            linear   INV 03-SEP-2025
DEFINITION  protein YIPF6 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193988
VERSION     XP_034193988.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338097.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..214
                     /product="protein YIPF6 isoform X3"
                     /calculated_mol_wt=23937
     Region          <31..210
                     /region_name="YIP1"
                     /note="Rab GTPase interacting factor, Golgi membrane
                     protein [Intracellular trafficking and secretion];
                     cl46908"
                     /db_xref="CDD:481248"
     CDS             1..214
                     /gene="LOC117610528"
                     /coded_by="XM_034338097.2:146..790"
                     /db_xref="GeneID:117610528"
ORIGIN      
        1 meyadsqnve gemtvgakqk snlgepdfnt ldepikdtil rdvcavgkkf yhvlypkekk
       61 sllkewdlwg plvlctfmam tlqgssdran dpndggpeft evfvivwigs mivtlnskll
      121 ggnisffqsi cvlgycllpt aialilcrii lmveqttflf slrfiismig fiwatyasmv
      181 flgdsqpagr kalavypifl fyfviswlvi srtr
//
LOCUS       XP_034194001             122 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase (ubiquinone) B14.5 B subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034194001
VERSION     XP_034194001.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338110.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..122
                     /product="NADH dehydrogenase (ubiquinone) B14.5 B subunit"
                     /calculated_mol_wt=13972
     Region          14..122
                     /region_name="NDUF_C2"
                     /note="NADH-ubiquinone oxidoreductase subunit b14.5b
                     (NDUFC2); pfam06374"
                     /db_xref="CDD:428908"
     CDS             1..122
                     /gene="ND-B14.5B"
                     /coded_by="XM_034338110.2:141..509"
                     /db_xref="GeneID:117610547"
ORIGIN      
        1 medqegnfpa qwaldlirgp tnykeslvtk yvtevtstlt gvgamfvkny lsnrpyyasi
       61 hwtiglgilg figgrtlvni mdlryakrda mmvdyikqhp erfpaprnkk faeiiepwyp
      121 ir
//
LOCUS       XP_034194034             733 aa            linear   INV 03-SEP-2025
DEFINITION  LON peptidase N-terminal domain and RING finger protein 3 isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194034
VERSION     XP_034194034.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194034.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..733
                     /product="LON peptidase N-terminal domain and RING finger
                     protein 3 isoform X1"
                     /calculated_mol_wt=81977
     Region          97..137
                     /region_name="RING-HC_LONFs_rpt1"
                     /note="first RING finger, HC subclass, found in the LON
                     peptidase N-terminal domain and RING finger protein
                     family; cd16513"
                     /db_xref="CDD:438176"
     Region          155..>268
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(164..165,168..169,171,195,198..199,202..203,
                     205..206,229,232..233,236..237,240)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          164..188
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          193..223
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          228..251
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <347..437
                     /region_name="PEX10"
                     /note="RING-finger-containing E3 ubiquitin ligase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5574"
                     /db_xref="CDD:227861"
     Region          397..441
                     /region_name="RING-HC_LONFs_rpt2"
                     /note="second RING finger, HC subclass, found in the LON
                     peptidase N-terminal domain and RING finger protein
                     family; cd16514"
                     /db_xref="CDD:438177"
     Region          500..696
                     /region_name="LON/PUA"
                     /note="Uncharacterized conserved protein, LON_N-like
                     domain, ASCH/PUA-like superfamily [Function unknown];
                     COG2802"
                     /db_xref="CDD:442054"
     CDS             1..733
                     /gene="LOC117610572"
                     /coded_by="XM_034338143.2:1497..3698"
                     /db_xref="GeneID:117610572"
ORIGIN      
        1 mtelakeafa srnyrlavem yernlkhqtp tfemlvgygd slakcgrire sigvyarcla
       61 vgsipperlk hlanalldel sgaattassr rkietsfacp vcegtlcqpv ttncghiyck
      121 ncvepgkncr vcgqkiglvg etnvlvqrlv ekwwpreaea srarhegdil mkeghlaqal
      181 erynlavhla pnsplhlsnr ahvllllnrp qasltdadha vrlrpdygkg hyrrgmalsa
      241 lgrheealfa lcisvaidkn pqavrhdlik ysfvnylqml ykvlssghrc yslssrtpyn
      301 ltegvsrvrs rhqllslkrn rrvalhgsdc ednssggeee ytqtvsrrll sttvpqnntk
      361 lqdgldrlyq dieklkrlea kpaeilfppl sggtageldc ilccrllwkp vttpcghtyc
      421 wmcldrcldy ssacplcvts ladylassqk tvtdfveral ktvapaeyas ratshrlelv
      481 qglgllgsee iavfvcttaf pcvacplfvy epryrlmvrr cvesgvrqfg iaaclnreat
      541 gtkryaeygt mleirdrvll kdgcsilstv ggrrfrvlsg gerdgydtaq veflrdtmvh
      601 edqllnllel hdkvrtkgrk wwdtvpssqq teiqrvfgrm peteedwprl pdgpswtwwl
      661 lailplgpql qvgilgttsl ekrlraiekt ldhmeqrqlt vapapseett tsssicrdeg
      721 gitdtpvsmv qqt
//
LOCUS       XP_034194038             725 aa            linear   INV 03-SEP-2025
DEFINITION  LON peptidase N-terminal domain and RING finger protein 3 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194038
VERSION     XP_034194038.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338147.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194038.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="LON peptidase N-terminal domain and RING finger
                     protein 3 isoform X2"
                     /calculated_mol_wt=80962
     Region          97..137
                     /region_name="RING-HC_LONFs_rpt1"
                     /note="first RING finger, HC subclass, found in the LON
                     peptidase N-terminal domain and RING finger protein
                     family; cd16513"
                     /db_xref="CDD:438176"
     Region          155..>274
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(164..165,168..169,171,195,198..199,202..203,
                     205..206,229,232..233,236..237,240)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          164..188
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          193..223
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          228..251
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          <339..429
                     /region_name="PEX10"
                     /note="RING-finger-containing E3 ubiquitin ligase
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5574"
                     /db_xref="CDD:227861"
     Region          389..433
                     /region_name="RING-HC_LONFs_rpt2"
                     /note="second RING finger, HC subclass, found in the LON
                     peptidase N-terminal domain and RING finger protein
                     family; cd16514"
                     /db_xref="CDD:438177"
     Region          492..688
                     /region_name="LON/PUA"
                     /note="Uncharacterized conserved protein, LON_N-like
                     domain, ASCH/PUA-like superfamily [Function unknown];
                     COG2802"
                     /db_xref="CDD:442054"
     CDS             1..725
                     /gene="LOC117610572"
                     /coded_by="XM_034338147.2:1495..3672"
                     /db_xref="GeneID:117610572"
ORIGIN      
        1 mtelakeafa srnyrlavem yernlkhqtp tfemlvgygd slakcgrire sigvyarcla
       61 vgsipperlk hlanalldel sgaattassr rkietsfacp vcegtlcqpv ttncghiyck
      121 ncvepgkncr vcgqkiglvg etnvlvqrlv ekwwpreaea srarhegdil mkeghlaqal
      181 erynlavhla pnsplhlsnr ahvllllnrp qasltdadha vrlrpdygkg hyrrgmalsa
      241 lgrheealfa lcisvaidkn pqavrhdlik mlykvlssgh rcyslssrtp ynltegvsrv
      301 rsrhqllslk rnrrvalhgs dcednssgge eeytqtvsrr llsttvpqnn tklqdgldrl
      361 yqdieklkrl eakpaeilfp plsggtagel dcilccrllw kpvttpcght ycwmcldrcl
      421 dyssacplcv tsladylass qktvtdfver alktvapaey asratshrle lvqglgllgs
      481 eeiavfvctt afpcvacplf vyepryrlmv rrcvesgvrq fgiaaclnre atgtkryaey
      541 gtmleirdrv llkdgcsils tvggrrfrvl sggerdgydt aqveflrdtm vhedqllnll
      601 elhdkvrtkg rkwwdtvpss qqteiqrvfg rmpeteedwp rlpdgpswtw wllailplgp
      661 qlqvgilgtt slekrlraie ktldhmeqrq ltvapapsee tttsssicrd eggitdtpvs
      721 mvqqt
//
LOCUS       XP_034194052             595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610585 [Osmia lignaria lignaria].
ACCESSION   XP_034194052
VERSION     XP_034194052.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338161.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194052.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="uncharacterized protein LOC117610585"
                     /calculated_mol_wt=68137
     Region          120..161
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(120..121,123..125,128..129,132,143..145,147,150)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(120,124,127..128,131..132,136,138..139,143..145,147)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          <279..>334
                     /region_name="PLC-beta_C"
                     /note="PLC-beta C terminal; pfam08703"
                     /db_xref="CDD:462571"
     CDS             1..595
                     /gene="LOC117610585"
                     /coded_by="XM_034338161.2:16..1803"
                     /db_xref="GeneID:117610585"
ORIGIN      
        1 mtldavqvsv lmqfqaered fseldassms gkieylekys iapspsrdyy glrvlgdqvi
       61 lylwrishgp hklpivrtts ldnftqdksl qdeirrifgk yllrhirqia sgrrntllal
      121 pkclikrlir ylavqdivkl sslshvakei fddnfvwetl ykkykpltkr qnerldfisy
      181 dwkrlfqqaq mqglikvpra rpvtkqakpn qkledftkts nfasrtiskp ipfenqsskk
      241 pvknvqkkii qnpiprkssd irldesimdk erekfeisek kastirslqm saqkntktlk
      301 qkstitries khemkdslrd kpsetklnkt qnkarkidak asnislktle eregnsvksk
      361 snnsrttkne ravsrtiqed qsktrtkgkg kkigqsksti fntatalldd pglrstvknd
      421 cldladliea slknmrsprs ildcnfdksk fcagdsaeki tehsrgllkn granvtldrl
      481 seksepltak svdsvkndas arsvaskykt knknefngtm efdayferyy nkhvvphntr
      541 eseenkrsil rktvdkmsvl rslgtknsyp kkigvdsvpq anryelkkav gnffk
//
LOCUS       XP_034194182            1139 aa            linear   INV 03-SEP-2025
DEFINITION  regulating synaptic membrane exocytosis protein fife isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034194182
VERSION     XP_034194182.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338291.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1139
                     /product="regulating synaptic membrane exocytosis protein
                     fife isoform X1"
                     /calculated_mol_wt=125819
     Region          84..138
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     Site            order(96..101,106,126)
                     /site_type="other"
                     /note="phosphatidylinositol 3-phosphate binding site
                     [chemical binding]"
                     /db_xref="CDD:277249"
     Region          499..589
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(517..523,538,541,570,574,577..578)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          647..778
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          989..1127
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1139
                     /gene="Fife"
                     /coded_by="XM_034338291.2:689..4108"
                     /db_xref="GeneID:117610657"
ORIGIN      
        1 mlpanvvsfv kkmvttqpds gttgsvtpdd ggttfskwrq lgsgvirdvt kfgpskvtpp
       61 eetqpkeett testktkskt gddcrvcrks fapdetsrtc cecqhkvced cacystttns
      121 ddpsswrcsv crrkiasrdq pivtqestds llevpvleal qrrhsdarlg cqsgsqiggl
      181 gsglapprsp elrrhsdvsp aslkelekfr kvagerrddl rwerdldrnk sratspdrhq
      241 vergratspq rvgvessvae psesmpidad edeerrrrar rtggtvrcks rmtrqrsydd
      301 emknvaamsg sggqhahpep glglpvqlpr rasaydvyaa pgvgglnama iaaaqqrasi
      361 saqggrkpee rptsrrssfr avkpvmpyev gaedesmmnl eassvpvvpp avvaqpvlvp
      421 deerrtrrrg sqlpdinaik alassapgva kvvpppavnr vaaedrelar qgslvdgesi
      481 kivihhadnd mtpwvnakrr lklrrepald kghrtagfgl rvvggktgad grtlayvmwt
      541 vpdgpaakag iqrgdkvlew ggvslvdrsf eevvqiterg ddmvelvveh aadtgdlped
      601 laavpssgkv sgnmgllmeg etdktpsspt rrklpktpeq iakekqvsgr vqiqvsyeae
      661 rkelivsvfm addlcaredt gygtlpeaya klvlvpicad qitlktavae ptqkpiwnat
      721 llfsgvdges fcssdkkrsi evtlwdfcpd gdnvflgecs vdleaalnnd racwyrledp
      781 rglraskspy csprssfsme iaqrllrkte lrersysddt qsdsgspelg flhpdhawha
      841 nsrrgssqse qlevepyeln rdysrslpgs rrssfqsqgg tdskrgsmge admpaivhyn
      901 rdrrrssftr pmrdpeeime glrslkaakr elgrtmslsg dkrresrrge rkdsvmnipe
      961 rfydrnsesd edekwshktr gengvdanlg rgqvppkgfk iiagaqngev klgfclskgt
     1021 levevicard icpgekeepd tyvktylrdg drwlqkrktr virhsrnpqy rqtlkysscd
     1081 algrnllvml wekkqafesn qglggaevsl drlpltkltv dwyplfpiht lgthtadsp
//
LOCUS       XP_034194188            1139 aa            linear   INV 03-SEP-2025
DEFINITION  regulating synaptic membrane exocytosis protein fife isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034194188
VERSION     XP_034194188.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338297.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1139
                     /product="regulating synaptic membrane exocytosis protein
                     fife isoform X1"
                     /calculated_mol_wt=125819
     Region          84..138
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     Site            order(96..101,106,126)
                     /site_type="other"
                     /note="phosphatidylinositol 3-phosphate binding site
                     [chemical binding]"
                     /db_xref="CDD:277249"
     Region          499..589
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(517..523,538,541,570,574,577..578)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          647..778
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          989..1127
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1139
                     /gene="Fife"
                     /coded_by="XM_034338297.2:563..3982"
                     /db_xref="GeneID:117610657"
ORIGIN      
        1 mlpanvvsfv kkmvttqpds gttgsvtpdd ggttfskwrq lgsgvirdvt kfgpskvtpp
       61 eetqpkeett testktkskt gddcrvcrks fapdetsrtc cecqhkvced cacystttns
      121 ddpsswrcsv crrkiasrdq pivtqestds llevpvleal qrrhsdarlg cqsgsqiggl
      181 gsglapprsp elrrhsdvsp aslkelekfr kvagerrddl rwerdldrnk sratspdrhq
      241 vergratspq rvgvessvae psesmpidad edeerrrrar rtggtvrcks rmtrqrsydd
      301 emknvaamsg sggqhahpep glglpvqlpr rasaydvyaa pgvgglnama iaaaqqrasi
      361 saqggrkpee rptsrrssfr avkpvmpyev gaedesmmnl eassvpvvpp avvaqpvlvp
      421 deerrtrrrg sqlpdinaik alassapgva kvvpppavnr vaaedrelar qgslvdgesi
      481 kivihhadnd mtpwvnakrr lklrrepald kghrtagfgl rvvggktgad grtlayvmwt
      541 vpdgpaakag iqrgdkvlew ggvslvdrsf eevvqiterg ddmvelvveh aadtgdlped
      601 laavpssgkv sgnmgllmeg etdktpsspt rrklpktpeq iakekqvsgr vqiqvsyeae
      661 rkelivsvfm addlcaredt gygtlpeaya klvlvpicad qitlktavae ptqkpiwnat
      721 llfsgvdges fcssdkkrsi evtlwdfcpd gdnvflgecs vdleaalnnd racwyrledp
      781 rglraskspy csprssfsme iaqrllrkte lrersysddt qsdsgspelg flhpdhawha
      841 nsrrgssqse qlevepyeln rdysrslpgs rrssfqsqgg tdskrgsmge admpaivhyn
      901 rdrrrssftr pmrdpeeime glrslkaakr elgrtmslsg dkrresrrge rkdsvmnipe
      961 rfydrnsesd edekwshktr gengvdanlg rgqvppkgfk iiagaqngev klgfclskgt
     1021 levevicard icpgekeepd tyvktylrdg drwlqkrktr virhsrnpqy rqtlkysscd
     1081 algrnllvml wekkqafesn qglggaevsl drlpltkltv dwyplfpiht lgthtadsp
//
LOCUS       XP_034194191            1136 aa            linear   INV 03-SEP-2025
DEFINITION  regulating synaptic membrane exocytosis protein fife isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034194191
VERSION     XP_034194191.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338300.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1136
                     /product="regulating synaptic membrane exocytosis protein
                     fife isoform X2"
                     /calculated_mol_wt=125387
     Region          84..138
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     Site            order(96..101,106,126)
                     /site_type="other"
                     /note="phosphatidylinositol 3-phosphate binding site
                     [chemical binding]"
                     /db_xref="CDD:277249"
     Region          496..586
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(514..520,535,538,567,571,574..575)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          644..775
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          986..1124
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1136
                     /gene="Fife"
                     /coded_by="XM_034338300.2:689..4099"
                     /db_xref="GeneID:117610657"
ORIGIN      
        1 mlpanvvsfv kkmvttqpds gttgsvtpdd ggttfskwrq lgsgvirdvt kfgpskvtpp
       61 eetqpkeett testktkskt gddcrvcrks fapdetsrtc cecqhkvced cacystttns
      121 ddpsswrcsv crrkiasrdq pivtqestds llevpvleal qrrhsdarlg cqsgsqiggl
      181 gsglapprsp elrrhsdvsp aslkelekva gerrddlrwe rdldrnksra tspdrhqver
      241 gratspqrvg vessvaepse smpidadede errrrarrtg gtvrcksrmt rqrsyddemk
      301 nvaamsgsgg qhahpepglg lpvqlprras aydvyaapgv gglnamaiaa aqqrasisaq
      361 ggrkpeerpt srrssfravk pvmpyevgae desmmnleas svpvvppavv aqpvlvpdee
      421 rrtrrrgsql pdinaikala ssapgvakvv pppavnrvaa edrelarqgs lvdgesikiv
      481 ihhadndmtp wvnakrrlkl rrepaldkgh rtagfglrvv ggktgadgrt layvmwtvpd
      541 gpaakagiqr gdkvlewggv slvdrsfeev vqitergddm velvvehaad tgdlpedlaa
      601 vpssgkvsgn mgllmegetd ktpssptrrk lpktpeqiak ekqvsgrvqi qvsyeaerke
      661 livsvfmadd lcaredtgyg tlpeayaklv lvpicadqit lktavaeptq kpiwnatllf
      721 sgvdgesfcs sdkkrsievt lwdfcpdgdn vflgecsvdl eaalnndrac wyrledprgl
      781 raskspycsp rssfsmeiaq rllrktelre rsysddtqsd sgspelgflh pdhawhansr
      841 rgssqseqle vepyelnrdy srslpgsrrs sfqsqggtds krgsmgeadm paivhynrdr
      901 rrssftrpmr dpeeimeglr slkaakrelg rtmslsgdkr resrrgerkd svmniperfy
      961 drnsesdede kwshktrgen gvdanlgrgq vppkgfkiia gaqngevklg fclskgtlev
     1021 evicardicp gekeepdtyv ktylrdgdrw lqkrktrvir hsrnpqyrqt lkysscdalg
     1081 rnllvmlwek kqafesnqgl ggaevsldrl pltkltvdwy plfpihtlgt htadsp
//
LOCUS       XP_034194195             980 aa            linear   INV 03-SEP-2025
DEFINITION  regulating synaptic membrane exocytosis protein fife isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034194195
VERSION     XP_034194195.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338304.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..980
                     /product="regulating synaptic membrane exocytosis protein
                     fife isoform X3"
                     /calculated_mol_wt=107469
     Region          84..138
                     /region_name="FYVE_like_SF"
                     /note="FYVE domain like superfamily; cl28890"
                     /db_xref="CDD:333710"
     Site            order(96..101,106,126)
                     /site_type="other"
                     /note="phosphatidylinositol 3-phosphate binding site
                     [chemical binding]"
                     /db_xref="CDD:277249"
     Region          499..589
                     /region_name="PDZ_canonical"
                     /note="canonical PDZ domain; cl49608"
                     /db_xref="CDD:483948"
     Site            order(517..523,538,541,570,574,577..578)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467153"
     Region          647..778
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..980
                     /gene="Fife"
                     /coded_by="XM_034338304.2:689..3631"
                     /db_xref="GeneID:117610657"
ORIGIN      
        1 mlpanvvsfv kkmvttqpds gttgsvtpdd ggttfskwrq lgsgvirdvt kfgpskvtpp
       61 eetqpkeett testktkskt gddcrvcrks fapdetsrtc cecqhkvced cacystttns
      121 ddpsswrcsv crrkiasrdq pivtqestds llevpvleal qrrhsdarlg cqsgsqiggl
      181 gsglapprsp elrrhsdvsp aslkelekfr kvagerrddl rwerdldrnk sratspdrhq
      241 vergratspq rvgvessvae psesmpidad edeerrrrar rtggtvrcks rmtrqrsydd
      301 emknvaamsg sggqhahpep glglpvqlpr rasaydvyaa pgvgglnama iaaaqqrasi
      361 saqggrkpee rptsrrssfr avkpvmpyev gaedesmmnl eassvpvvpp avvaqpvlvp
      421 deerrtrrrg sqlpdinaik alassapgva kvvpppavnr vaaedrelar qgslvdgesi
      481 kivihhadnd mtpwvnakrr lklrrepald kghrtagfgl rvvggktgad grtlayvmwt
      541 vpdgpaakag iqrgdkvlew ggvslvdrsf eevvqiterg ddmvelvveh aadtgdlped
      601 laavpssgkv sgnmgllmeg etdktpsspt rrklpktpeq iakekqvsgr vqiqvsyeae
      661 rkelivsvfm addlcaredt gygtlpeaya klvlvpicad qitlktavae ptqkpiwnat
      721 llfsgvdges fcssdkkrsi evtlwdfcpd gdnvflgecs vdleaalnnd racwyrledp
      781 rglraskspy csprssfsme iaqrllrkte lrersysddt qsdsgspelg flhpdhawha
      841 nsrrgssqse qlevepyeln rdysrslpgs rrssfqsqgg tdskrgsmge admpaivhyn
      901 rdrrrssftr pmrdpeeime glrslkaakr elgrtmslsg dkrrespcvv ptvtvwqcep
      961 vsprtgksas fplphsippr
//
LOCUS       XP_034194202             404 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger CCCH domain-containing protein 15 homolog isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034194202
VERSION     XP_034194202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338311.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="zinc finger CCCH domain-containing protein 15
                     homolog isoform X1"
                     /calculated_mol_wt=46284
     Region          1..289
                     /region_name="COG5252"
                     /note="Uncharacterized conserved protein, contains
                     CCCH-type Zn-finger protein [General function prediction
                     only]"
                     /db_xref="CDD:227577"
     CDS             1..404
                     /gene="LOC117610673"
                     /coded_by="XM_034338311.2:131..1345"
                     /db_xref="GeneID:117610673"
ORIGIN      
        1 mppkkapaps kkseqkkkek viedktfgik nkkgakqqkf iqqvekqvks ggvnprkled
       61 pnvkklekek klkeqkelal ifkpvqtqki dkgtdpksvv caffkqgqct kgdkckfshd
      121 lsverkaekr slycdmrddd dketdtmdkw dedklkevve kkhgggnrpt tdiickhfle
      181 avekakygwf wecpsgqkci yrhalppgfv lkkdkkkedk kdeisledli ekeranlgpn
      241 qtkitletfl awkkrklrek keqalkdeek rrndykagrq vgisgremfy fnpelaagdg
      301 iddgdeaiss yvreeeeede geerveyrel dmdrlafeps eidtsgitva sidrlkddkv
      361 tnnedstvvt vgegaaalai nenlfkeedl egleeeledl dlee
//
LOCUS       XP_034194209             403 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger CCCH domain-containing protein 15 homolog isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034194209
VERSION     XP_034194209.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338318.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="zinc finger CCCH domain-containing protein 15
                     homolog isoform X2"
                     /calculated_mol_wt=46185
     Region          1..289
                     /region_name="COG5252"
                     /note="Uncharacterized conserved protein, contains
                     CCCH-type Zn-finger protein [General function prediction
                     only]"
                     /db_xref="CDD:227577"
     CDS             1..403
                     /gene="LOC117610673"
                     /coded_by="XM_034338318.2:134..1345"
                     /db_xref="GeneID:117610673"
ORIGIN      
        1 mppkkapaps kkseqkkkek viedktfgik nkkgakqqkf iqqvekqvks ggvnprkled
       61 pnvkklekek klkeqkelal ifkpvqtqki dkgtdpksvv caffkqgqct kgdkckfshd
      121 lsverkaekr slycdmrddd dketdtmdkw dedklkevve kkhgggnrpt tdiickhfle
      181 avekakygwf wecpsgqkci yrhalppgfv lkkdkkkedk kdeisledli ekeranlgpn
      241 qtkitletfl awkkrklrek keqalkdeek rrndykagrq vgisgremfy fnpelaagdg
      301 iddgdeaiss yvreeeeede geerveyrel dmdrlafeps eidtsgitva sidrlkddkv
      361 tnnedstvtv gegaaalain enlfkeedle gleeeledld lee
//
LOCUS       XP_034194215             356 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial transcription factor B1 [Osmia lignaria lignaria].
ACCESSION   XP_034194215
VERSION     XP_034194215.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338324.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..356
                     /product="mitochondrial transcription factor B1"
                     /calculated_mol_wt=41201
     Region          19..300
                     /region_name="RsmA"
                     /note="16S rRNA A1518 and A1519 N6-dimethyltransferase
                     RsmA/KsgA/DIM1 (may also have DNA glycosylase/AP lyase
                     activity) [Translation, ribosomal structure and
                     biogenesis]; COG0030"
                     /db_xref="CDD:439801"
     CDS             1..356
                     /gene="mtTFB1"
                     /coded_by="XM_034338324.2:167..1237"
                     /db_xref="GeneID:117610683"
ORIGIN      
        1 mtalrlpplp tikevlkiyk lraikqlsqn fifdqnltdk iirktgnlad cyaleigpgp
       61 ggltrsilkq hpkkltviek derfkpmldm lidsyaavng emeviyndim kvdmsdlfpt
      121 svktawedrs pkirlignlp fnvstpliik wlhaisekrg pwafgrtrmt ltfqkevaer
      181 iiaepsniqr crlsvmaqaw trpvlhfiip grafipkpdv dvglvsfvpl vtprtrhefk
      241 ifekvnrhif sfrqkysirg ietlfpveyr telgqmmykl sdldpqlrsv qltvedidkl
      301 vtaykyllek ypdirmyeyr asrrivslsk mkdlkvidyv edinvedtqa ipeiin
//
LOCUS       XP_034194646            1340 aa            linear   INV 03-SEP-2025
DEFINITION  sushi domain containing 2 mesh isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194646
VERSION     XP_034194646.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338755.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194646.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1340
                     /product="sushi domain containing 2 mesh isoform X1"
                     /calculated_mol_wt=153330
     Region          272..433
                     /region_name="NIDO"
                     /note="Nidogen-like; cl02648"
                     /db_xref="CDD:470642"
     Region          670..813
                     /region_name="AMOP"
                     /note="AMOP domain; pfam03782"
                     /db_xref="CDD:461048"
     Region          833..1009
                     /region_name="VWD"
                     /note="von Willebrand factor type D domain; pfam00094"
                     /db_xref="CDD:459671"
     Region          1133..1183
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1143,1160)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     CDS             1..1340
                     /gene="mesh"
                     /coded_by="XM_034338755.2:62..4084"
                     /db_xref="GeneID:117610906"
ORIGIN      
        1 mrnklrpdrv sgfaksilvg cfllsilivr isaqidnetf enafgldete fpdnqesiyt
       61 gsntddekve aqnplseven lnggeggnne ddvkevngdv panvnensgq ennakkeisv
      121 shgisgkmag gksekyvryd sdlpeadrya prsddsvldy vltetrlkei rskfmywffd
      181 kggdddegdy qkqiqsstpq ihknfnfqlp fykfrynytr ismngfiefs dppvhytypl
      241 vfpvkdwpkk ndpsfigmff skcrigkirk tdidqrkpgv yfrmerdlen rkdqfgvemr
      301 ervkwdireg iigtesfepk havivtwknm sfaggidnsl yktntfqmvl atdevttyvi
      361 fnyldiqwts hteaggdttg geggvaafvg fnagngtery eykpysqkst irdlpgrgwa
      421 ngfpgrhifr idekimpgtc nkdiaganld lvfapesgnm lggtivnitg pcfnetemir
      481 cmfetvvvtg tvidknraic vqpfvraegy vrfaisigds ktynwkgkyf ietpataaek
      541 lkvsntvhek npatieitwk phnltnnlna giqialwgyr etkttpefey idileqgitn
      601 igsyiirpas lrnrtnryqq dmnfgfiqln ltdpmkqtgl nitpnlwsrp iplawyfgpq
      661 wermygsawa qklcdkwimn drylknfage valcpciveh altdkgrflp dydcdkdsnl
      721 dcmynkhayh cvrtgapsmd gseqqccydh kgylmltydq qwgsrphrsh nlgyypwdea
      781 nkvptlshwh hdvapfymcc lwqeehavgc etfrferrps qdciayqspa vatvfgdphv
      841 vtfdgleytf ngkgefvlvr vnnmkdkldi qarfeqlpnn vygevkatql tsiaargnns
      901 atievrlrpk haqwryrldv faneqrvyfd rralkfqhfs gvvvytptyi lnqseviimf
      961 dtgagveive negymtarvy lpwtylnktk glfgtwnfdi tddlttpdgn qvsgtnlnhf
     1021 etvhkdfgln wmledkedaq kggalftrkf grtssyysnr tfeaewrktp aeiipsnrsy
     1081 diqraidlcg dlyqcqydya lslnrdlahf tknyyntytq irdlnmnqrv vscgvletpr
     1141 fgrksnfffv pgtkvtfecn qdftligdqr rvctpegrwn vpeygytecl rqieyaqrta
     1201 wttmgiifav sipiigcilg sfflirknqk qggsvnswry serssgvdne stllkqatpl
     1261 ktyerpispv sdtstaastv kkphsydkvy rtheplpnrp nvdfedkdwd lkepnsptds
     1321 eksmtesirk agsptkesdv
//
LOCUS       XP_034194649            1270 aa            linear   INV 03-SEP-2025
DEFINITION  sushi domain containing 2 mesh isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194649
VERSION     XP_034194649.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338758.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194649.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="sushi domain containing 2 mesh isoform X2"
                     /calculated_mol_wt=145689
     Region          272..433
                     /region_name="NIDO"
                     /note="Extracellular domain of unknown function in nidogen
                     (entactin) and hypothetical proteins; smart00539"
                     /db_xref="CDD:214712"
     Region          670..813
                     /region_name="AMOP"
                     /note="AMOP domain; pfam03782"
                     /db_xref="CDD:461048"
     Region          833..1009
                     /region_name="VWD"
                     /note="von Willebrand factor type D domain; pfam00094"
                     /db_xref="CDD:459671"
     Region          1133..1183
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(1143,1160)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     CDS             1..1270
                     /gene="mesh"
                     /coded_by="XM_034338758.2:62..3874"
                     /db_xref="GeneID:117610906"
ORIGIN      
        1 mrnklrpdrv sgfaksilvg cfllsilivr isaqidnetf enafgldete fpdnqesiyt
       61 gsntddekve aqnplseven lnggeggnne ddvkevngdv panvnensgq ennakkeisv
      121 shgisgkmag gksekyvryd sdlpeadrya prsddsvldy vltetrlkei rskfmywffd
      181 kggdddegdy qkqiqsstpq ihknfnfqlp fykfrynytr ismngfiefs dppvhytypl
      241 vfpvkdwpkk ndpsfigmff skcrigkirk tdidqrkpgv yfrmerdlen rkdqfgvemr
      301 ervkwdireg iigtesfepk havivtwknm sfaggidnsl yktntfqmvl atdevttyvi
      361 fnyldiqwts hteaggdttg geggvaafvg fnagngtery eykpysqkst irdlpgrgwa
      421 ngfpgrhifr idekimpgtc nkdiaganld lvfapesgnm lggtivnitg pcfnetemir
      481 cmfetvvvtg tvidknraic vqpfvraegy vrfaisigds ktynwkgkyf ietpataaek
      541 lkvsntvhek npatieitwk phnltnnlna giqialwgyr etkttpefey idileqgitn
      601 igsyiirpas lrnrtnryqq dmnfgfiqln ltdpmkqtgl nitpnlwsrp iplawyfgpq
      661 wermygsawa qklcdkwimn drylknfage valcpciveh altdkgrflp dydcdkdsnl
      721 dcmynkhayh cvrtgapsmd gseqqccydh kgylmltydq qwgsrphrsh nlgyypwdea
      781 nkvptlshwh hdvapfymcc lwqeehavgc etfrferrps qdciayqspa vatvfgdphv
      841 vtfdgleytf ngkgefvlvr vnnmkdkldi qarfeqlpnn vygevkatql tsiaargnns
      901 atievrlrpk haqwryrldv faneqrvyfd rralkfqhfs gvvvytptyi lnqseviimf
      961 dtgagveive negymtarvy lpwtylnktk glfgtwnfdi tddlttpdgn qvsgtnlnhf
     1021 etvhkdfgln wmledkedaq kggalftrkf grtssyysnr tfeaewrktp aeiipsnrsy
     1081 diqraidlcg dlyqcqydya lslnrdlahf tknyyntytq irdlnmnqrv vscgvletpr
     1141 fgrksnfffv pgtkvtfecn qdftligdqr rvctpegrwn vpeygytecl rqqeyssrqa
     1201 gvttgiifac lipilllivc vavrvlkkqr eqreqeeslh rarsaelqrl rkikeddept
     1261 tysskatdin
//
LOCUS       XP_034194670             307 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit Pop2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194670
VERSION     XP_034194670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338779.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="CCR4-NOT transcription complex subunit Pop2
                     isoform X1"
                     /calculated_mol_wt=34507
     Region          33..287
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; cl23804"
                     /db_xref="CDD:474062"
     CDS             1..307
                     /gene="Pop2"
                     /coded_by="XM_034338779.2:214..1137"
                     /db_xref="GeneID:117610915"
ORIGIN      
        1 mpsatgtvke gggnpigqqk ggatmpsnee cgirdvwghn leeefrtirq vvqqyqyiam
       61 dtefpgvvar pigefrtsad yqyqllrcnv dllriiqlgl tfldesgntp ggsyttwqfn
      121 fkfnlqedmy aqdsidmlqn sgiqfkkhee egidpldfae llmtsgivlv ddikwlsfhs
      181 gydfgyllkl ltdqnlphee seffellriy fptiydvkyl mkscknlkgg lqevaeqlei
      241 qrvgpqhqag sdslltgmvf fkmremffed niddakycgh lyglgtsfvv ngsggyldsn
      301 gdnssss
//
LOCUS       XP_034194659             307 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit Pop2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194659
VERSION     XP_034194659.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338768.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="CCR4-NOT transcription complex subunit Pop2
                     isoform X1"
                     /calculated_mol_wt=34507
     Region          33..287
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; cl23804"
                     /db_xref="CDD:474062"
     CDS             1..307
                     /gene="Pop2"
                     /coded_by="XM_034338768.2:221..1144"
                     /db_xref="GeneID:117610915"
ORIGIN      
        1 mpsatgtvke gggnpigqqk ggatmpsnee cgirdvwghn leeefrtirq vvqqyqyiam
       61 dtefpgvvar pigefrtsad yqyqllrcnv dllriiqlgl tfldesgntp ggsyttwqfn
      121 fkfnlqedmy aqdsidmlqn sgiqfkkhee egidpldfae llmtsgivlv ddikwlsfhs
      181 gydfgyllkl ltdqnlphee seffellriy fptiydvkyl mkscknlkgg lqevaeqlei
      241 qrvgpqhqag sdslltgmvf fkmremffed niddakycgh lyglgtsfvv ngsggyldsn
      301 gdnssss
//
LOCUS       XP_034194667             307 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit Pop2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194667
VERSION     XP_034194667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="CCR4-NOT transcription complex subunit Pop2
                     isoform X1"
                     /calculated_mol_wt=34507
     Region          33..287
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; cl23804"
                     /db_xref="CDD:474062"
     CDS             1..307
                     /gene="Pop2"
                     /coded_by="XM_034338776.2:133..1056"
                     /db_xref="GeneID:117610915"
ORIGIN      
        1 mpsatgtvke gggnpigqqk ggatmpsnee cgirdvwghn leeefrtirq vvqqyqyiam
       61 dtefpgvvar pigefrtsad yqyqllrcnv dllriiqlgl tfldesgntp ggsyttwqfn
      121 fkfnlqedmy aqdsidmlqn sgiqfkkhee egidpldfae llmtsgivlv ddikwlsfhs
      181 gydfgyllkl ltdqnlphee seffellriy fptiydvkyl mkscknlkgg lqevaeqlei
      241 qrvgpqhqag sdslltgmvf fkmremffed niddakycgh lyglgtsfvv ngsggyldsn
      301 gdnssss
//
LOCUS       XP_034194674             302 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit Pop2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194674
VERSION     XP_034194674.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338783.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..302
                     /product="CCR4-NOT transcription complex subunit Pop2
                     isoform X2"
                     /calculated_mol_wt=33992
     Region          28..282
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; cl23804"
                     /db_xref="CDD:474062"
     CDS             1..302
                     /gene="Pop2"
                     /coded_by="XM_034338783.2:214..1122"
                     /db_xref="GeneID:117610915"
ORIGIN      
        1 mpsatgggnp igqqkggatm psneecgird vwghnleeef rtirqvvqqy qyiamdtefp
       61 gvvarpigef rtsadyqyql lrcnvdllri iqlgltflde sgntpggsyt twqfnfkfnl
      121 qedmyaqdsi dmlqnsgiqf kkheeegidp ldfaellmts givlvddikw lsfhsgydfg
      181 yllklltdqn lpheeseffe llriyfptiy dvkylmksck nlkgglqeva eqleiqrvgp
      241 qhqagsdsll tgmvffkmre mffednidda kycghlyglg tsfvvngsgg yldsngdnss
      301 ss
//
LOCUS       XP_034194678             302 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit Pop2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194678
VERSION     XP_034194678.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338787.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..302
                     /product="CCR4-NOT transcription complex subunit Pop2
                     isoform X2"
                     /calculated_mol_wt=33992
     Region          28..282
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; cl23804"
                     /db_xref="CDD:474062"
     CDS             1..302
                     /gene="Pop2"
                     /coded_by="XM_034338787.2:221..1129"
                     /db_xref="GeneID:117610915"
ORIGIN      
        1 mpsatgggnp igqqkggatm psneecgird vwghnleeef rtirqvvqqy qyiamdtefp
       61 gvvarpigef rtsadyqyql lrcnvdllri iqlgltflde sgntpggsyt twqfnfkfnl
      121 qedmyaqdsi dmlqnsgiqf kkheeegidp ldfaellmts givlvddikw lsfhsgydfg
      181 yllklltdqn lpheeseffe llriyfptiy dvkylmksck nlkgglqeva eqleiqrvgp
      241 qhqagsdsll tgmvffkmre mffednidda kycghlyglg tsfvvngsgg yldsngdnss
      301 ss
//
LOCUS       XP_034194685             302 aa            linear   INV 03-SEP-2025
DEFINITION  CCR4-NOT transcription complex subunit Pop2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194685
VERSION     XP_034194685.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338794.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..302
                     /product="CCR4-NOT transcription complex subunit Pop2
                     isoform X2"
                     /calculated_mol_wt=33992
     Region          28..282
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; cl23804"
                     /db_xref="CDD:474062"
     CDS             1..302
                     /gene="Pop2"
                     /coded_by="XM_034338794.2:126..1034"
                     /db_xref="GeneID:117610915"
ORIGIN      
        1 mpsatgggnp igqqkggatm psneecgird vwghnleeef rtirqvvqqy qyiamdtefp
       61 gvvarpigef rtsadyqyql lrcnvdllri iqlgltflde sgntpggsyt twqfnfkfnl
      121 qedmyaqdsi dmlqnsgiqf kkheeegidp ldfaellmts givlvddikw lsfhsgydfg
      181 yllklltdqn lpheeseffe llriyfptiy dvkylmksck nlkgglqeva eqleiqrvgp
      241 qhqagsdsll tgmvffkmre mffednidda kycghlyglg tsfvvngsgg yldsngdnss
      301 ss
//
LOCUS       XP_076546537             725 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546537
VERSION     XP_076546537.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690422.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="anaphase-promoting complex subunit 4 isoform X1"
                     /calculated_mol_wt=81822
     Region          17..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          20..109
                     /region_name="ANAPC4_WD40"
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
                     pfam12894"
                     /db_xref="CDD:403945"
     Region          61..100
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          231..411
                     /region_name="ANAPC4"
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
                     pfam12896"
                     /db_xref="CDD:463744"
     CDS             1..725
                     /gene="APC4"
                     /coded_by="XM_076690422.1:1118..3295"
                     /db_xref="GeneID:117610703"
ORIGIN      
        1 magsvrqlee rqlpaevtrm qwspkmdlla ianikgevtl hrltwqrvwl lspqeeadsv
       61 vnlawrpdgk flavcyetsk viclvdienk niiqrtklal hneitcmtwl pltnlesdtv
      121 lngnkpnmlp tgeylpplps lnrsfgqepe rkeflsqtld vlflgldngs vamyvfgmfy
      181 cgtisvgqdr ileisggfgk pmwvtwkdng gikacrlwcp lleqstaffk vaqaqaniey
      241 lmdylsrtlm aiseawetil lemdeklary aetnppggva adflellmig iptpnlesfl
      301 lrdltekglk klghsiemcy sniqklvlkn ltnvgmalvy qlaemrgmvr lggpyellgf
      361 anesiitnal haseaflaks seiqqvidhs mrdykaffrw lyvvilrltd erippevsrv
      421 sqqeltfiae flrgfdktep gvggrkgvnl eklgqylrre nlqtcltpeg sewtamlsen
      481 hclrdhplii kqdlnfsllq shaklisaih nvfreayqgl vehftvttia ltpsvsftss
      541 qiatnndgll vatcdmdhkv lrlfrikcsh tehislnfkl gtidvdhrse syskacidgt
      601 vidlqfysqe ylsllllnkh nhasfliqlp lkypeifenq dknavsladl ignnwprpfq
      661 gisarriavs garkvaailn esnrkirlle tevepedeed evdeedgvnd smldtthekp
      721 ttssq
//
LOCUS       XP_034194289             725 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194289
VERSION     XP_034194289.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338398.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194289.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="anaphase-promoting complex subunit 4 isoform X1"
                     /calculated_mol_wt=81822
     Region          17..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          20..109
                     /region_name="ANAPC4_WD40"
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
                     pfam12894"
                     /db_xref="CDD:403945"
     Region          61..100
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          231..411
                     /region_name="ANAPC4"
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
                     pfam12896"
                     /db_xref="CDD:463744"
     CDS             1..725
                     /gene="APC4"
                     /coded_by="XM_034338398.2:567..2744"
                     /db_xref="GeneID:117610703"
ORIGIN      
        1 magsvrqlee rqlpaevtrm qwspkmdlla ianikgevtl hrltwqrvwl lspqeeadsv
       61 vnlawrpdgk flavcyetsk viclvdienk niiqrtklal hneitcmtwl pltnlesdtv
      121 lngnkpnmlp tgeylpplps lnrsfgqepe rkeflsqtld vlflgldngs vamyvfgmfy
      181 cgtisvgqdr ileisggfgk pmwvtwkdng gikacrlwcp lleqstaffk vaqaqaniey
      241 lmdylsrtlm aiseawetil lemdeklary aetnppggva adflellmig iptpnlesfl
      301 lrdltekglk klghsiemcy sniqklvlkn ltnvgmalvy qlaemrgmvr lggpyellgf
      361 anesiitnal haseaflaks seiqqvidhs mrdykaffrw lyvvilrltd erippevsrv
      421 sqqeltfiae flrgfdktep gvggrkgvnl eklgqylrre nlqtcltpeg sewtamlsen
      481 hclrdhplii kqdlnfsllq shaklisaih nvfreayqgl vehftvttia ltpsvsftss
      541 qiatnndgll vatcdmdhkv lrlfrikcsh tehislnfkl gtidvdhrse syskacidgt
      601 vidlqfysqe ylsllllnkh nhasfliqlp lkypeifenq dknavsladl ignnwprpfq
      661 gisarriavs garkvaailn esnrkirlle tevepedeed evdeedgvnd smldtthekp
      721 ttssq
//
LOCUS       XP_034194280             725 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194280
VERSION     XP_034194280.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338389.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194280.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="anaphase-promoting complex subunit 4 isoform X1"
                     /calculated_mol_wt=81822
     Region          17..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          20..109
                     /region_name="ANAPC4_WD40"
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
                     pfam12894"
                     /db_xref="CDD:403945"
     Region          61..100
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          231..411
                     /region_name="ANAPC4"
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
                     pfam12896"
                     /db_xref="CDD:463744"
     CDS             1..725
                     /gene="APC4"
                     /coded_by="XM_034338389.2:130..2307"
                     /db_xref="GeneID:117610703"
ORIGIN      
        1 magsvrqlee rqlpaevtrm qwspkmdlla ianikgevtl hrltwqrvwl lspqeeadsv
       61 vnlawrpdgk flavcyetsk viclvdienk niiqrtklal hneitcmtwl pltnlesdtv
      121 lngnkpnmlp tgeylpplps lnrsfgqepe rkeflsqtld vlflgldngs vamyvfgmfy
      181 cgtisvgqdr ileisggfgk pmwvtwkdng gikacrlwcp lleqstaffk vaqaqaniey
      241 lmdylsrtlm aiseawetil lemdeklary aetnppggva adflellmig iptpnlesfl
      301 lrdltekglk klghsiemcy sniqklvlkn ltnvgmalvy qlaemrgmvr lggpyellgf
      361 anesiitnal haseaflaks seiqqvidhs mrdykaffrw lyvvilrltd erippevsrv
      421 sqqeltfiae flrgfdktep gvggrkgvnl eklgqylrre nlqtcltpeg sewtamlsen
      481 hclrdhplii kqdlnfsllq shaklisaih nvfreayqgl vehftvttia ltpsvsftss
      541 qiatnndgll vatcdmdhkv lrlfrikcsh tehislnfkl gtidvdhrse syskacidgt
      601 vidlqfysqe ylsllllnkh nhasfliqlp lkypeifenq dknavsladl ignnwprpfq
      661 gisarriavs garkvaailn esnrkirlle tevepedeed evdeedgvnd smldtthekp
      721 ttssq
//
LOCUS       XP_034194300             725 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194300
VERSION     XP_034194300.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338409.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194300.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="anaphase-promoting complex subunit 4 isoform X1"
                     /calculated_mol_wt=81822
     Region          17..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          20..109
                     /region_name="ANAPC4_WD40"
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
                     pfam12894"
                     /db_xref="CDD:403945"
     Region          61..100
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          231..411
                     /region_name="ANAPC4"
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
                     pfam12896"
                     /db_xref="CDD:463744"
     CDS             1..725
                     /gene="APC4"
                     /coded_by="XM_034338409.2:134..2311"
                     /db_xref="GeneID:117610703"
ORIGIN      
        1 magsvrqlee rqlpaevtrm qwspkmdlla ianikgevtl hrltwqrvwl lspqeeadsv
       61 vnlawrpdgk flavcyetsk viclvdienk niiqrtklal hneitcmtwl pltnlesdtv
      121 lngnkpnmlp tgeylpplps lnrsfgqepe rkeflsqtld vlflgldngs vamyvfgmfy
      181 cgtisvgqdr ileisggfgk pmwvtwkdng gikacrlwcp lleqstaffk vaqaqaniey
      241 lmdylsrtlm aiseawetil lemdeklary aetnppggva adflellmig iptpnlesfl
      301 lrdltekglk klghsiemcy sniqklvlkn ltnvgmalvy qlaemrgmvr lggpyellgf
      361 anesiitnal haseaflaks seiqqvidhs mrdykaffrw lyvvilrltd erippevsrv
      421 sqqeltfiae flrgfdktep gvggrkgvnl eklgqylrre nlqtcltpeg sewtamlsen
      481 hclrdhplii kqdlnfsllq shaklisaih nvfreayqgl vehftvttia ltpsvsftss
      541 qiatnndgll vatcdmdhkv lrlfrikcsh tehislnfkl gtidvdhrse syskacidgt
      601 vidlqfysqe ylsllllnkh nhasfliqlp lkypeifenq dknavsladl ignnwprpfq
      661 gisarriavs garkvaailn esnrkirlle tevepedeed evdeedgvnd smldtthekp
      721 ttssq
//
LOCUS       XP_034194320             725 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194320
VERSION     XP_034194320.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338429.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194320.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..725
                     /product="anaphase-promoting complex subunit 4 isoform X1"
                     /calculated_mol_wt=81822
     Region          17..55
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          20..109
                     /region_name="ANAPC4_WD40"
                     /note="Anaphase-promoting complex subunit 4 WD40 domain;
                     pfam12894"
                     /db_xref="CDD:403945"
     Region          61..100
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          231..411
                     /region_name="ANAPC4"
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
                     pfam12896"
                     /db_xref="CDD:463744"
     CDS             1..725
                     /gene="APC4"
                     /coded_by="XM_034338429.2:417..2594"
                     /db_xref="GeneID:117610703"
ORIGIN      
        1 magsvrqlee rqlpaevtrm qwspkmdlla ianikgevtl hrltwqrvwl lspqeeadsv
       61 vnlawrpdgk flavcyetsk viclvdienk niiqrtklal hneitcmtwl pltnlesdtv
      121 lngnkpnmlp tgeylpplps lnrsfgqepe rkeflsqtld vlflgldngs vamyvfgmfy
      181 cgtisvgqdr ileisggfgk pmwvtwkdng gikacrlwcp lleqstaffk vaqaqaniey
      241 lmdylsrtlm aiseawetil lemdeklary aetnppggva adflellmig iptpnlesfl
      301 lrdltekglk klghsiemcy sniqklvlkn ltnvgmalvy qlaemrgmvr lggpyellgf
      361 anesiitnal haseaflaks seiqqvidhs mrdykaffrw lyvvilrltd erippevsrv
      421 sqqeltfiae flrgfdktep gvggrkgvnl eklgqylrre nlqtcltpeg sewtamlsen
      481 hclrdhplii kqdlnfsllq shaklisaih nvfreayqgl vehftvttia ltpsvsftss
      541 qiatnndgll vatcdmdhkv lrlfrikcsh tehislnfkl gtidvdhrse syskacidgt
      601 vidlqfysqe ylsllllnkh nhasfliqlp lkypeifenq dknavsladl ignnwprpfq
      661 gisarriavs garkvaailn esnrkirlle tevepedeed evdeedgvnd smldtthekp
      721 ttssq
//
LOCUS       XP_034194338             619 aa            linear   INV 03-SEP-2025
DEFINITION  anaphase-promoting complex subunit 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194338
VERSION     XP_034194338.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338447.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194338.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..619
                     /product="anaphase-promoting complex subunit 4 isoform X2"
                     /calculated_mol_wt=69640
     Region          125..305
                     /region_name="ANAPC4"
                     /note="Anaphase-promoting complex, cyclosome, subunit 4;
                     pfam12896"
                     /db_xref="CDD:463744"
     CDS             1..619
                     /gene="APC4"
                     /coded_by="XM_034338447.2:407..2266"
                     /db_xref="GeneID:117610703"
ORIGIN      
        1 mtwlpltnle sdtvlngnkp nmlptgeylp plpslnrsfg qeperkefls qtldvlflgl
       61 dngsvamyvf gmfycgtisv gqdrileisg gfgkpmwvtw kdnggikacr lwcplleqst
      121 affkvaqaqa nieylmdyls rtlmaiseaw etillemdek laryaetnpp ggvaadflel
      181 lmigiptpnl esfllrdlte kglkklghsi emcysniqkl vlknltnvgm alvyqlaemr
      241 gmvrlggpye llgfanesii tnalhaseaf laksseiqqv idhsmrdyka ffrwlyvvil
      301 rltderippe vsrvsqqelt fiaeflrgfd ktepgvggrk gvnleklgqy lrrenlqtcl
      361 tpegsewtam lsenhclrdh pliikqdlnf sllqshakli saihnvfrea yqglvehftv
      421 ttialtpsvs ftssqiatnn dgllvatcdm dhkvlrlfri kcshtehisl nfklgtidvd
      481 hrsesyskac idgtvidlqf ysqeylslll lnkhnhasfl iqlplkypei fenqdknavs
      541 ladlignnwp rpfqgisarr iavsgarkva ailnesnrki rlletevepe deedevdeed
      601 gvndsmldtt hekpttssq
//
LOCUS       XP_034194349             511 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylglucosamine-6-sulfatase [Osmia lignaria lignaria].
ACCESSION   XP_034194349
VERSION     XP_034194349.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338458.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="N-acetylglucosamine-6-sulfatase"
                     /calculated_mol_wt=58047
     Region          36..478
                     /region_name="G6S"
                     /note="glucosamine (N-acetyl)-6-sulfatase(G6S, GNS) AND
                     sulfatase 1(SULF1); cd16147"
                     /db_xref="CDD:293766"
     Site            order(43..44,79,137,139,223,315..316)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:293766"
     Site            order(43..44,79,137,139,223,316)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:293766"
     CDS             1..511
                     /gene="LOC117610729"
                     /coded_by="XM_034338458.2:154..1689"
                     /db_xref="GeneID:117610729"
ORIGIN      
        1 mnfmkllqiy ikfpkmivll fyiyiscisl sqtaenivlv vtddldifld gmtpmtntln
       61 ligkqgatft ncfvatpvcc pnrasiltgk yqhnhlvlnn sisggcnsiq wqrlqepntf
      121 gahlkremly ttfyagkyln qygghavggt ahvpegwdww aglvgnsryy nyslsingte
      181 kkfgdkpney ltdvindlam eflktrnvdn qpflmvltpp aphapftpav rhtdkykdvk
      241 akrtpnfntl tqtdkhwlvq qgpsplpdkl lpklddiyrr rwetllavde litniyqalk
      301 sqnllnntym iftsdngyhi gqfsmpidkr qpyetdirvp lliqgpgitp skisapvstv
      361 dlfatileia giqlpsdgas llkktlpedr tllveyrger sddppsgcps dsdpnvalcn
      421 kdlackcqdt vnntftcirr mspqynnifc vfdddkhfie aynittdnfq mknvgysmkr
      481 hhrykfrkcl kkmstckgee cvltnmedmy m
//
LOCUS       XP_034194372             217 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610735 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194372
VERSION     XP_034194372.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338481.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="uncharacterized protein LOC117610735 isoform X1"
                     /calculated_mol_wt=24434
     Region          29..113
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     CDS             1..217
                     /gene="LOC117610735"
                     /coded_by="XM_034338481.2:247..900"
                     /db_xref="GeneID:117610735"
ORIGIN      
        1 mqqmsikcfl llysllfllk ngvhgvvtvt evpaghlael pclssddyhr fmfwqltddn
       61 riigpgnpmd enkydyevlt gklmikgvst aesgfykcvs kgvsdqsain ihvvelivkk
      121 dwedvwendf etnllrgmta vmvvvvglav vlfiittkrr rnrkffdlee srenspakyt
      181 pnvmarptap spipedggid nealdidfpk vfnqmqk
//
LOCUS       XP_034194362             217 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610735 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194362
VERSION     XP_034194362.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338471.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="uncharacterized protein LOC117610735 isoform X1"
                     /calculated_mol_wt=24434
     Region          29..113
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     CDS             1..217
                     /gene="LOC117610735"
                     /coded_by="XM_034338471.2:630..1283"
                     /db_xref="GeneID:117610735"
ORIGIN      
        1 mqqmsikcfl llysllfllk ngvhgvvtvt evpaghlael pclssddyhr fmfwqltddn
       61 riigpgnpmd enkydyevlt gklmikgvst aesgfykcvs kgvsdqsain ihvvelivkk
      121 dwedvwendf etnllrgmta vmvvvvglav vlfiittkrr rnrkffdlee srenspakyt
      181 pnvmarptap spipedggid nealdidfpk vfnqmqk
//
LOCUS       XP_034194381             215 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610735 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194381
VERSION     XP_034194381.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338490.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="uncharacterized protein LOC117610735 isoform X2"
                     /calculated_mol_wt=24178
     Region          27..111
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     CDS             1..215
                     /gene="LOC117610735"
                     /coded_by="XM_034338490.2:141..788"
                     /db_xref="GeneID:117610735"
ORIGIN      
        1 mmsikcflll ysllfllkng vhgvvtvtev paghlaelpc lssddyhrfm fwqltddnri
       61 igpgnpmden kydyevltgk lmikgvstae sgfykcvskg vsdqsainih vvelivkkdw
      121 edvwendfet nllrgmtavm vvvvglavvl fiittkrrrn rkffdleesr enspakytpn
      181 vmarptapsp ipedggidne aldidfpkvf nqmqk
//
LOCUS       XP_034194389             214 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610735 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194389
VERSION     XP_034194389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..214
                     /product="uncharacterized protein LOC117610735 isoform X3"
                     /calculated_mol_wt=24047
     Region          26..110
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     CDS             1..214
                     /gene="LOC117610735"
                     /coded_by="XM_034338498.2:436..1080"
                     /db_xref="GeneID:117610735"
ORIGIN      
        1 msikcfllly sllfllkngv hgvvtvtevp aghlaelpcl ssddyhrfmf wqltddnrii
       61 gpgnpmdenk ydyevltgkl mikgvstaes gfykcvskgv sdqsainihv velivkkdwe
      121 dvwendfetn llrgmtavmv vvvglavvlf iittkrrrnr kffdleesre nspakytpnv
      181 marptapspi pedggidnea ldidfpkvfn qmqk
//
LOCUS       XP_076546538             214 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610735 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546538
VERSION     XP_076546538.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690423.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..214
                     /product="uncharacterized protein LOC117610735 isoform X3"
                     /calculated_mol_wt=24047
     Region          26..110
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     CDS             1..214
                     /gene="LOC117610735"
                     /coded_by="XM_076690423.1:151..795"
                     /db_xref="GeneID:117610735"
ORIGIN      
        1 msikcfllly sllfllkngv hgvvtvtevp aghlaelpcl ssddyhrfmf wqltddnrii
       61 gpgnpmdenk ydyevltgkl mikgvstaes gfykcvskgv sdqsainihv velivkkdwe
      121 dvwendfetn llrgmtavmv vvvglavvlf iittkrrrnr kffdleesre nspakytpnv
      181 marptapspi pedggidnea ldidfpkvfn qmqk
//
LOCUS       XP_034194398             214 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610735 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194398
VERSION     XP_034194398.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338507.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..214
                     /product="uncharacterized protein LOC117610735 isoform X3"
                     /calculated_mol_wt=24047
     Region          26..110
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     CDS             1..214
                     /gene="LOC117610735"
                     /coded_by="XM_034338507.2:539..1183"
                     /db_xref="GeneID:117610735"
ORIGIN      
        1 msikcfllly sllfllkngv hgvvtvtevp aghlaelpcl ssddyhrfmf wqltddnrii
       61 gpgnpmdenk ydyevltgkl mikgvstaes gfykcvskgv sdqsainihv velivkkdwe
      121 dvwendfetn llrgmtavmv vvvglavvlf iittkrrrnr kffdleesre nspakytpnv
      181 marptapspi pedggidnea ldidfpkvfn qmqk
//
LOCUS       XP_034194651             472 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610911 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194651
VERSION     XP_034194651.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194651.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..472
                     /product="uncharacterized protein LOC117610911 isoform X1"
                     /calculated_mol_wt=54981
     CDS             1..472
                     /gene="LOC117610911"
                     /coded_by="XM_034338760.2:872..2290"
                     /db_xref="GeneID:117610911"
ORIGIN      
        1 messpgkiik rkykeesske eeagnstdsa kvgnmaepmt dknargldqw krgredkevs
       61 natvgsksnv kdlktktkge nngkdvkkdt kankvriies teikqkykii ddigknkete
      121 nkehglgkst dkeeisenkv rehayyktke esealyvael esgeyticvs lnkeklsnkr
      181 kynlvkiyni inilgakire irmmafsrte itlgtrrean riieygrkke agyeayiprr
      241 krtrrgvtke wedtleelkn cvmtgqgdfe aqrlkrrrlr ggkavwedsd avlltfkgds
      301 lptrlyigqg hvwlkiepfi esvkqcfrcf ryghlqavck srfrrykmcs eefhgdckke
      361 qkcvncgeah rsnareclif qreseikktm ayknvsysqa reilrdkytg rrfdldiese
      421 kdfpklrteq rgksdkwerl nvpernrgqn vwnrdtgyrr nqervssdes eg
//
LOCUS       XP_034194654             142 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610911 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194654
VERSION     XP_034194654.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338763.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..142
                     /product="uncharacterized protein LOC117610911 isoform X2"
                     /calculated_mol_wt=15798
     CDS             1..142
                     /gene="LOC117610911"
                     /coded_by="XM_034338763.2:871..1299"
                     /db_xref="GeneID:117610911"
ORIGIN      
        1 messpgkiik rkykeesske eeagnstdsa kvgnmaepmt dknargldqw krgredkevs
       61 natvgsksnv kdlktktkge nngkdvkkdt kankvriies teikqkykii ddigknkete
      121 nkehglgkst dkeeisenkv qk
//
LOCUS       XP_076546539             236 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305723 [Osmia lignaria lignaria].
ACCESSION   XP_076546539
VERSION     XP_076546539.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690424.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="uncharacterized protein LOC143305723"
                     /calculated_mol_wt=27026
     Region          <1..116
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     CDS             1..236
                     /gene="LOC143305723"
                     /coded_by="XM_076690424.1:2154..2864"
                     /db_xref="GeneID:143305723"
ORIGIN      
        1 mnpgatiftt eavavaealr lysqsgdgrr llvysnsasv lkvleaaknk dikdivkmkr
       61 geivakimke lceiylmknl plnetvnrkk tpvvfvwvpa hrgiagnera daaakrdmlv
      121 nlvgnawkef ateirneary kgvkyfseid tnkqrrkswf ekfvtqdrrt isvlsriran
      181 hynlseslar khlvedhgcd crehvedlah vlwecekydh irdefvakmr ekgfsg
//
LOCUS       XP_076546540             864 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611775 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546540
VERSION     XP_076546540.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690425.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..864
                     /product="uncharacterized protein LOC117611775 isoform X1"
                     /calculated_mol_wt=97021
     Region          139..>474
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          617..817
                     /region_name="FReD"
                     /note="Fibrinogen-related domains (FReDs); C terminal
                     globular domain of fibrinogen. Fibrinogen is involved in
                     blood clotting, being activated by thrombin to assemble
                     into fibrin clots. The N-termini of 2 times 3 chains come
                     together to form a globular...; cd00087"
                     /db_xref="CDD:238040"
     Site            740
                     /site_type="other"
                     /note="gamma-gamma dimer interface [polypeptide binding]"
                     /db_xref="CDD:238040"
     Site            order(772,774,776)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238040"
     Site            order(780,783..784,793..794)
                     /site_type="active"
                     /note="polymerization pocket [active]"
                     /db_xref="CDD:238040"
     CDS             1..864
                     /gene="LOC117611775"
                     /coded_by="XM_076690425.1:1291..3885"
                     /db_xref="GeneID:117611775"
ORIGIN      
        1 mfrlkrngil lrtsnrllsd pravsvgtll fiiltiisfs cssisssyrh sfsfssslpf
       61 plrhrnhrsi vfvrfidpri tlfssimrea iiittcllga vfagsprlpr tsaderntrn
      121 vsnnggnvag ntmpasvrns qrnreqylfn vfevivstle sklqrienld kavehlmrrv
      181 ealdsrvndn idktdavitk lrtldlklfn aqssffpvep aygtrtsdnk ngsaaksgst
      241 iqtinqkqed teasetlgek llsldqkvsd idnklidlkn qldnnflqnd dinaeasekk
      301 pismsvieit kamsnevinh vtieienlrq attnidrklq fhtnlvsdrl gaiqnmvtdv
      361 hdailvgata ngqqifnvtp avevspkesk idrlvqqmyp mitvsdkidq iwnvvvgtks
      421 svdnllpksd ellsqtqrqe raiseiqqdf rlktnkiien ledvenrlrk qvddvpgtlg
      481 qrpipaelll dptidrlvey dssryvgste eittetsatp vtpttivsss vsmsmsssts
      541 snivpvtgfn nsniisagln nsinsttspg ivgsssnsqr ssaknrgvif psvknkpspa
      601 nstfatdafl sfkdvkgysc vdllnagmrd sgvyylqirg ttywflkvyc eqdiaeggwt
      661 viqrrddfge prenfnrdwa dykngfgdpa refwlgneni ymltnnedym lrveledfeg
      721 nkryaqyshf kiysegeyyk leidgydgna gdslndpwyg snnspfstyn rdndrsslnc
      781 asmlkggwww kscgrglngl ylndpqdlta rqgtwfyclp lfsivtnvih iryrmvsler
      841 lglhsktryd ddqtkrthgs sigy
//
LOCUS       XP_034195935             842 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611775 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195935
VERSION     XP_034195935.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340044.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195935.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..842
                     /product="uncharacterized protein LOC117611775 isoform X2"
                     /calculated_mol_wt=94453
     Region          139..>474
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          617..836
                     /region_name="FReD"
                     /note="Fibrinogen-related domains (FReDs); C terminal
                     globular domain of fibrinogen. Fibrinogen is involved in
                     blood clotting, being activated by thrombin to assemble
                     into fibrin clots. The N-termini of 2 times 3 chains come
                     together to form a globular...; cd00087"
                     /db_xref="CDD:238040"
     Site            740
                     /site_type="other"
                     /note="gamma-gamma dimer interface [polypeptide binding]"
                     /db_xref="CDD:238040"
     Site            order(772,774,776)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238040"
     Site            order(780,783..784,793..794)
                     /site_type="active"
                     /note="polymerization pocket [active]"
                     /db_xref="CDD:238040"
     CDS             1..842
                     /gene="LOC117611775"
                     /coded_by="XM_034340044.2:1291..3819"
                     /db_xref="GeneID:117611775"
ORIGIN      
        1 mfrlkrngil lrtsnrllsd pravsvgtll fiiltiisfs cssisssyrh sfsfssslpf
       61 plrhrnhrsi vfvrfidpri tlfssimrea iiittcllga vfagsprlpr tsaderntrn
      121 vsnnggnvag ntmpasvrns qrnreqylfn vfevivstle sklqrienld kavehlmrrv
      181 ealdsrvndn idktdavitk lrtldlklfn aqssffpvep aygtrtsdnk ngsaaksgst
      241 iqtinqkqed teasetlgek llsldqkvsd idnklidlkn qldnnflqnd dinaeasekk
      301 pismsvieit kamsnevinh vtieienlrq attnidrklq fhtnlvsdrl gaiqnmvtdv
      361 hdailvgata ngqqifnvtp avevspkesk idrlvqqmyp mitvsdkidq iwnvvvgtks
      421 svdnllpksd ellsqtqrqe raiseiqqdf rlktnkiien ledvenrlrk qvddvpgtlg
      481 qrpipaelll dptidrlvey dssryvgste eittetsatp vtpttivsss vsmsmsssts
      541 snivpvtgfn nsniisagln nsinsttspg ivgsssnsqr ssaknrgvif psvknkpspa
      601 nstfatdafl sfkdvkgysc vdllnagmrd sgvyylqirg ttywflkvyc eqdiaeggwt
      661 viqrrddfge prenfnrdwa dykngfgdpa refwlgneni ymltnnedym lrveledfeg
      721 nkryaqyshf kiysegeyyk leidgydgna gdslndpwyg snnspfstyn rdndrsslnc
      781 asmlkggwww kscgrglngl ylndpqdlta rqgivwfrwr gwdytlkram mmikpkgpta
      841 ap
//
LOCUS       XP_034195936             739 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611775 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195936
VERSION     XP_034195936.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340045.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195936.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..739
                     /product="uncharacterized protein LOC117611775 isoform X3"
                     /calculated_mol_wt=82602
     Region          139..>474
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          617..>722
                     /region_name="FReD"
                     /note="Fibrinogen-related domains (FReDs); C terminal
                     globular domain of fibrinogen. Fibrinogen is involved in
                     blood clotting, being activated by thrombin to assemble
                     into fibrin clots. The N-termini of 2 times 3 chains come
                     together to form a globular...; cl00085"
                     /db_xref="CDD:412152"
     CDS             1..739
                     /gene="LOC117611775"
                     /coded_by="XM_034340045.2:1291..3510"
                     /db_xref="GeneID:117611775"
ORIGIN      
        1 mfrlkrngil lrtsnrllsd pravsvgtll fiiltiisfs cssisssyrh sfsfssslpf
       61 plrhrnhrsi vfvrfidpri tlfssimrea iiittcllga vfagsprlpr tsaderntrn
      121 vsnnggnvag ntmpasvrns qrnreqylfn vfevivstle sklqrienld kavehlmrrv
      181 ealdsrvndn idktdavitk lrtldlklfn aqssffpvep aygtrtsdnk ngsaaksgst
      241 iqtinqkqed teasetlgek llsldqkvsd idnklidlkn qldnnflqnd dinaeasekk
      301 pismsvieit kamsnevinh vtieienlrq attnidrklq fhtnlvsdrl gaiqnmvtdv
      361 hdailvgata ngqqifnvtp avevspkesk idrlvqqmyp mitvsdkidq iwnvvvgtks
      421 svdnllpksd ellsqtqrqe raiseiqqdf rlktnkiien ledvenrlrk qvddvpgtlg
      481 qrpipaelll dptidrlvey dssryvgste eittetsatp vtpttivsss vsmsmsssts
      541 snivpvtgfn nsniisagln nsinsttspg ivgsssnsqr ssaknrgvif psvknkpspa
      601 nstfatdafl sfkdvkgysc vdllnagmrd sgvyylqirg ttywflkvyc eqdiaeggwt
      661 viqrrddfge prenfnrdwa dykngfgdpa refwlgneni ymltnnedym lrveledfeg
      721 trntlisksi rkenitnwk
//
LOCUS       XP_034195938             306 aa            linear   INV 03-SEP-2025
DEFINITION  trypsin 3A1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195938
VERSION     XP_034195938.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340047.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195938.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..306
                     /product="trypsin 3A1 isoform X1"
                     /calculated_mol_wt=33843
     Region          41..276
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; smart00020"
                     /db_xref="CDD:214473"
     Site            42
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(83,141,238)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(232,253,255)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..306
                     /gene="LOC117611777"
                     /coded_by="XM_034340047.2:922..1842"
                     /db_xref="GeneID:117611777"
ORIGIN      
        1 mlllflllcs fqafiqpvdv ilsnelkrsr kilstksvaa rivggevtdi rkypflvsvq
       61 rrhishtcgg tyiaprfvls aahcmvrpas rgkwvpenph rfyviagaty vyfpawssiq
      121 lelidgifph rdfqftgmnh diglfrlrka llinsfvkya tlppaydani fqsytasctv
      181 vgwgvsmpgs nrgygtylrh vnvslipsdk cpveglnteq hlcagwlegg rdacqgdsgg
      241 pllcngtqig ivswgkgcar pnfpgvyarl dlyldwlnet mqrniacqrn ildvivilta
      301 visstl
//
LOCUS       XP_034195939             222 aa            linear   INV 03-SEP-2025
DEFINITION  trypsin-3 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195939
VERSION     XP_034195939.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340048.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195939.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="trypsin-3 isoform X2"
                     /calculated_mol_wt=24459
     Region          10..196
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            order(148,169,171)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..222
                     /gene="LOC117611777"
                     /coded_by="XM_034340048.2:228..896"
                     /db_xref="GeneID:117611777"
ORIGIN      
        1 mvrpasrgkw vpenphrfyv iagatyvyfp awssiqleli dgifphrdfq ftgmnhdigl
       61 frlrkallin sfvkyatlpp aydanifqsy tasctvvgwg vsmpgsnrgy gtylrhvnvs
      121 lipsdkcpve glnteqhlca gwleggrdac qgdsggpllc ngtqigivsw gkgcarpnfp
      181 gvyarldlyl dwlnetmqrn iacqrnildv iviltaviss tl
//
LOCUS       XP_034195940             280 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611778 [Osmia lignaria lignaria].
ACCESSION   XP_034195940
VERSION     XP_034195940.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340049.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="uncharacterized protein LOC117611778"
                     /calculated_mol_wt=31531
     Region          36..116
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          135..240
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          156..160
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          170..174
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          202..206
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          233..236
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..280
                     /gene="LOC117611778"
                     /coded_by="XM_034340049.2:147..989"
                     /db_xref="GeneID:117611778"
ORIGIN      
        1 mprqpahwrk fvlvyifqlf syatstvhvq liapryvtyg stavlhcnhs vsdaslhkve
       61 fmkddktilr yikdrkppfl kwgvdganme ymangttikl kdvrfeasgs ysclvsmttp
      121 iyaqpsdivq mkvivpqten pkitfkkgmy mvgeileanc tssaahpvph ltwyingkev
      181 dislvnhyph trhkdqlmsa taklmievsa lhagengyle iscystipdy plhqeqyadv
      241 reetvsveik papiaessra rgwplatllc ilctmhkiip
//
LOCUS       XP_034195551             535 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-glucosyltransferase 2-like [Osmia lignaria lignaria].
ACCESSION   XP_034195551
VERSION     XP_034195551.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195551.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..535
                     /product="UDP-glucosyltransferase 2-like"
                     /calculated_mol_wt=62147
     Region          14..471
                     /region_name="Glycosyltransferase_GTB-type"
                     /note="glycosyltransferase family 1 and related proteins
                     with GTB topology; cl10013"
                     /db_xref="CDD:471961"
     Site            order(42..43,45,194,197..198,202,245,309,374,376,378..379,
                     382)
                     /site_type="active"
                     /db_xref="CDD:340817"
     Site            order(42,309,374,376,378..379)
                     /site_type="active"
                     /note="TDP-binding site [active]"
                     /db_xref="CDD:340817"
     Site            43
                     /site_type="active"
                     /note="acceptor substrate-binding pocket [active]"
                     /db_xref="CDD:340817"
     CDS             1..535
                     /gene="LOC117611643"
                     /coded_by="XM_034339660.2:95..1702"
                     /db_xref="GeneID:117611643"
ORIGIN      
        1 myefssiqrt khlvpflvll vslsnqvtce riliitqapf yshesvmrsl glalhkrghe
       61 ivmitsfptk nlslinftei dfsenydrsl nfkfhddifn ypsimginmh iknylynftr
      121 yffehpdvqk iyrknskeki dliiaesays hgvfyvarvl nipvigtttl dfhahhsymw
      181 gipplpshls hwefdgliep nmslclkvrn fiytmlvlhd wynfeveqvh alvksylgnd
      241 tpsfselrrn islflvnhdl llgfakpvtp nviyfngfhi menppplpsd mdeflrkahd
      301 gfiymnlgsi vsssllsnit irsifnvfsr lpykilwkfe tnnvlpkhpn iftskwipqq
      361 svlarpntkl fvyqgglqst eeaihygvpi vgipifvdqi frikklvshg vgrmleikky
      421 deetfretin dvlnnerykt kmlklrelmn dkpydsknht lwwieyvmrq kevphlhfsg
      481 addpcitsal vsfginnslv neytdiklte sakiqgsnyk ffhltlegki vgglr
//
LOCUS       XP_034194907            1410 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034194907
VERSION     XP_034194907.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339016.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194907.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1410
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X1"
                     /calculated_mol_wt=155699
     Region          534..633
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <827..1057
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(835,837,841..842,845..847,849..850,854,857,936,938,
                     940,944..945,948..950,952..953,957,960,974,976,978,
                     982..983,986..988,990..991,995,998)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          835..936
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          938..974
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          976..1005
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1217..1333
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1329..1400
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1410
                     /gene="LOC117611103"
                     /coded_by="XM_034339016.2:1720..5952"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mndmsaihre scgddcycvi crelkdcrma gnaeggaqgr reginfilrr glpfvtrsps
       61 rewletdlkr edvsleekch spevrpvrkt dkdvahktvi yfgdtkqrek enrlgqrdvs
      121 spitsrdesr msdarkvetd gfeqgknlte nedtkvthei vvnvspsred vlkieedesq
      181 vedywslpgd nsgfkadwsf vqqwrlrgpg ggggggsgre lycppyskdf tesstkngvh
      241 nmvhmvaerd tdsvaptptp qhphhsqhhh lslheiralq rrpectgnss sdenrssgha
      301 smsdtgghts ssspphrhhr ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp
      361 aklqvpwsgs glediklaiq qltmrshkss stysslsgse ssepavrrlm rhssletint
      421 nvtsadefvw vdshnrlvel qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr
      481 alvrvgretq rlakpvglcs khevysafki vlcpaladsc tkaclraaam favsgdqlkq
      541 skasrsglql pvgrflrwms dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl
      601 tatmlehaia nsgdlwgllq pyahlnagrt asgalamprw asvsslnsss sssrsgrdat
      661 ssalepsllt tcvgsmseli dliskvaqag rspiplttka lnalfyymrc sqlehgergs
      721 giqelayera yvvlpplvew lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic
      781 feevsvcskr iddteyvrll tmdmafkmlt sgradliaqa mpllpsakin tvndngftal
      841 miacingdet avlalldaga dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim
      901 ssgksmqsss sasslpspsn nvssnvccnq tafnaetqhw taltytalmg hcniarille
      961 rgaaveggak psedkctvtp lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq
     1021 rgcysaisva tahgqrsclh qllshplnfs akrgekevls leeilaegsa asspqqqtvd
     1081 grgnrregke pvfnkvqtka lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah
     1141 emrldsvidq llqdflqvcp ddystqfvqe clpllfnifr yskkegttll ladifctcfg
     1201 wepikpirdt tlssgsridp kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss
     1261 klcegnppiv qindiryhif qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry
     1321 ceaqcssmvd ldnivsmyih akvynatqll eycqgfllqn mvalltydds vkrllfakkl
     1381 pnhdvlagll ltlqariktr rsqqqnkika
//
LOCUS       XP_076546541            1408 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_076546541
VERSION     XP_076546541.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690426.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1408
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X2"
                     /calculated_mol_wt=155458
     Region          532..631
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <825..1055
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(833,835,839..840,843..845,847..848,852,855,934,936,
                     938,942..943,946..948,950..951,955,958,972,974,976,
                     980..981,984..986,988..989,993,996)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          833..934
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          936..972
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          974..1003
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1215..1331
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1327..1398
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1408
                     /gene="LOC117611103"
                     /coded_by="XM_076690426.1:1720..5946"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mndmsaihre scgddcycvi crelkdcrma gnaeggaqgr reginfilrr glpfvtrsps
       61 rewletdlkr edvsleekch spevrpvrkt dkdvahktvi yfgdtkqrek enrlgqrdvs
      121 spitsrdesr msdarkvetd gfeqgknlte nedtkvthei vvnvspsred vlkieedesq
      181 vedywslpgd nsgfkadwsf vqqwrlrgpg ggggggsgre lycppyskdf tesstkngvh
      241 nmvhmvaerd tdsvaptptp qhphhsqhhh lslheiralq rrpectgnss sdenrssgha
      301 smsdtgghts ssspphrhhr ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp
      361 akvpwsgsgl ediklaiqql tmrshkssst ysslsgsess epavrrlmrh ssletintnv
      421 tsadefvwvd shnrlvelqq lpwthhdvlr vlqngrtreh meqvsmetip rlsyllqral
      481 vrvgretqrl akpvglcskh evysafkivl cpaladsctk aclraaamfa vsgdqlkqsk
      541 asrsglqlpv grflrwmsdv rlgrmiheya aiyltagien lleeillqci ptdphttlta
      601 tmlehaians gdlwgllqpy ahlnagrtas galamprwas vsslnsssss srsgrdatss
      661 alepsllttc vgsmselidl iskvaqagrs piplttkaln alfyymrcsq lehgergsgi
      721 qelayerayv vlpplvewlr vatahaehrh glvvdqddin qaarlllpgv dcpvrpicfe
      781 evsvcskrid dteyvrlltm dmafkmltsg radliaqamp llpsakintv ndngftalmi
      841 acingdetav lalldagadl niespppstg qlantpskvp vtpgtsstrs pvtpssimss
      901 gksmqssssa sslpspsnnv ssnvccnqta fnaetqhwta ltytalmghc niarillerg
      961 aaveggakps edkctvtplq aatatgnnem valllahgaq pflstlikds fsysgsaqrg
     1021 cysaisvata hgqrsclhql lshplnfsak rgekevlsle eilaegsaas spqqqtvdgr
     1081 gnrregkepv fnkvqtkalq eamyhsaesn hlditmelrg lkvgwtlhcw mhslgtahem
     1141 rldsvidqll qdflqvcpdd ystqfvqecl pllfnifrys kkegttllla difctcfgwe
     1201 pikpirdttl ssgsridpkf vnnpelsdvq frvegrvfyg hkivlvtssp rfrnmlsskl
     1261 cegnppivqi ndiryhifqm vmeflyhggc atlevnqsdv lelmaaanff qldgllryce
     1321 aqcssmvdld nivsmyihak vynatqlley cqgfllqnmv alltyddsvk rllfakklpn
     1381 hdvlaglllt lqariktrrs qqqnkika
//
LOCUS       XP_076546542            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546542
VERSION     XP_076546542.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690427.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690427.1:3841..7653"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546543            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546543
VERSION     XP_076546543.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690428.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690428.1:3893..7705"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546544            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546544
VERSION     XP_076546544.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690429.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690429.1:3891..7703"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546545            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546545
VERSION     XP_076546545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690430.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690430.1:3121..6933"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546546            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546546
VERSION     XP_076546546.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690431.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690431.1:3789..7601"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546547            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546547
VERSION     XP_076546547.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690432.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690432.1:3945..7757"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546548            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546548
VERSION     XP_076546548.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690433.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690433.1:3887..7699"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546549            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546549
VERSION     XP_076546549.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690434.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690434.1:1857..5669"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546550            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546550
VERSION     XP_076546550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690435.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690435.1:1169..4981"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546551            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546551
VERSION     XP_076546551.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690436.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690436.1:1580..5392"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_076546552            1270 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and BTB/POZ domain-containing protein 2 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_076546552
VERSION     XP_076546552.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690437.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1270
                     /product="ankyrin repeat and BTB/POZ domain-containing
                     protein 2 isoform X3"
                     /calculated_mol_wt=138917
     Region          394..493
                     /region_name="HFD_ABTB2-like"
                     /note="histone-fold domain found in ankyrin repeat and
                     BTB/POZ domain-containing protein 2 (ABTB2) and similar
                     proteins; cd22913"
                     /db_xref="CDD:467038"
     Region          <687..917
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(695,697,701..702,705..707,709..710,714,717,796,798,
                     800,804..805,808..810,812..813,817,820,834,836,838,
                     842..843,846..848,850..851,855,858)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          695..796
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          798..834
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          836..865
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1077..1193
                     /region_name="BTB_POZ_ABTB2-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in Ankyrin repeat
                     and BTB/POZ domain-containing protein 2 (ABTB2) and
                     similar proteins; cd18297"
                     /db_xref="CDD:349606"
     Region          1189..1260
                     /region_name="BACK_ABTB2_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     ankyrin repeat and BTB/POZ domain-containing protein 2
                     (ABTB2) and similar proteins; cd18491"
                     /db_xref="CDD:350566"
     CDS             1..1270
                     /gene="LOC117611103"
                     /coded_by="XM_076690437.1:545..4357"
                     /db_xref="GeneID:117611103"
ORIGIN      
        1 mnverptmdg cgqqplfltg tsgsggissp qqqtvqtgap pehyggplsl sicisdsghe
       61 ilrpkprrpg ggggggsgre lycppyskdf tesstkngvh nmvhmvaerd tdsvaptptp
      121 qhphhsqhhh lslheiralq rrpectgnss sdenrssgha smsdtgghts ssspphrhhr
      181 ahspqqlnsv peddrlsasv tqrngrsrsg qnrnrhratp aklqvpwsgs glediklaiq
      241 qltmrshkss stysslsgse ssepavrrlm rhssletint nvtsadefvw vdshnrlvel
      301 qqlpwthhdv lrvlqngrtr ehmeqvsmet iprlsyllqr alvrvgretq rlakpvglcs
      361 khevysafki vlcpaladsc tkaclraaam favsgdqlkq skasrsglql pvgrflrwms
      421 dvrlgrmihe yaaiyltagi enlleeillq ciptdphttl tatmlehaia nsgdlwgllq
      481 pyahlnagrt asgalamprw asvsslnsss sssrsgrdat ssalepsllt tcvgsmseli
      541 dliskvaqag rspiplttka lnalfyymrc sqlehgergs giqelayera yvvlpplvew
      601 lrvatahaeh rhglvvdqdd inqaarlllp gvdcpvrpic feevsvcskr iddteyvrll
      661 tmdmafkmlt sgradliaqa mpllpsakin tvndngftal miacingdet avlalldaga
      721 dlniesppps tgqlantpsk vpvtpgtsst rspvtpssim ssgksmqsss sasslpspsn
      781 nvssnvccnq tafnaetqhw taltytalmg hcniarille rgaaveggak psedkctvtp
      841 lqaatatgnn emvalllahg aqpflstlik dsfsysgsaq rgcysaisva tahgqrsclh
      901 qllshplnfs akrgekevls leeilaegsa asspqqqtvd grgnrregke pvfnkvqtka
      961 lqeamyhsae snhlditmel rglkvgwtlh cwmhslgtah emrldsvidq llqdflqvcp
     1021 ddystqfvqe clpllfnifr yskkegttll ladifctcfg wepikpirdt tlssgsridp
     1081 kfvnnpelsd vqfrvegrvf yghkivlvts sprfrnmlss klcegnppiv qindiryhif
     1141 qmvmeflyhg gcatlevnqs dvlelmaaan ffqldgllry ceaqcssmvd ldnivsmyih
     1201 akvynatqll eycqgfllqn mvalltydds vkrllfakkl pnhdvlagll ltlqariktr
     1261 rsqqqnkika
//
LOCUS       XP_034194911            1103 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611109 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194911
VERSION     XP_034194911.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1103
                     /product="uncharacterized protein LOC117611109 isoform X1"
                     /calculated_mol_wt=118029
     Region          99..247
                     /region_name="Spt20"
                     /note="Spt20 family; pfam12090"
                     /db_xref="CDD:463459"
     CDS             1..1103
                     /gene="LOC117611109"
                     /coded_by="XM_034339020.2:471..3782"
                     /db_xref="GeneID:117611109"
ORIGIN      
        1 migaacsled acrqaqdmin qvskcwknvq gqnslnltsd kpsvqrkeqn hskhsiqskl
       61 trlyfeeltk vphatpdsvl nnlenecdll grlvqreglh tlivnlyagn kgyslavrns
      121 dkgnqydkns ilaetqlmgy eqgellscid ngqlpamlae qletnhshlf ydgciiaevr
      181 dyrktfpqtk aevhhvllkp ttqsvlsdvs tltsdgdwsh eerlmleshl vaatqgplcl
      241 dpnpipslat trlrqsksll tdhqlirqak kfsqvtvnrk rkleqlaqpe gltiqdlmqk
      301 lkakrgvvtt tacpppsltn ppllppntpi dvlrfakaye rpretkdclp qvieeyilet
      361 ggsqgeidhi klsilqrpsn seylgelymd knhregekng sacrftlgtr vlanhyiqqf
      421 teifteegrk nvrikhvvpg qvprvtctpg mqrahqqaqq akaaqlvaqh lqqaqsqhva
      481 qqilsraqgq lntlagqvas iktiteatts aqnnltcvds snipqviaqs dasslspgqs
      541 ltnggsnast svqidnstms vtdgsscngs gilvfqqkst nninnatstn vpvlqaqlqa
      601 gtqncvtetv nqnqneipfk khstnpaita lvtslmnsaq qfqqqaaana aaaamnsntq
      661 tpnksnnaai lsllnsspan ltqqkvqprr islnasipsr vlsshgnvin vpnttgqvrv
      721 slsstltgql gckqqpvkat avrlarvqdp tstlglsmpg lsallagtps adnpipgins
      781 gssllerlta sssqnnqaat smttppstnv nlqgvnltsl snsinglqnv qvsfpglsqp
      841 itmslnvsat tgsvtptgvi vslpissatn tcttvssmva ttvvttaiag stptvvianp
      901 anthlslpia qiipsgvkgl sqqnirssns vtlsqggqai qlvgsqrprl nhmtrqvqpn
      961 qvkstvlpnq lvtvaktvta sqlilsgnkq vltpimaatk pvlmasqttp ssqvvpankq
     1021 tlltkslhar astghcsqqt qslgvatlsq qqlqrtlvkp vqlqqlqcla aqhkvavatg
     1081 naqnrtqies qrtststpge dpa
//
LOCUS       XP_034194920            1102 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611109 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194920
VERSION     XP_034194920.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339029.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1102
                     /product="uncharacterized protein LOC117611109 isoform X2"
                     /calculated_mol_wt=117958
     Region          99..247
                     /region_name="Spt20"
                     /note="Spt20 family; pfam12090"
                     /db_xref="CDD:463459"
     CDS             1..1102
                     /gene="LOC117611109"
                     /coded_by="XM_034339029.2:471..3779"
                     /db_xref="GeneID:117611109"
ORIGIN      
        1 migaacsled acrqaqdmin qvskcwknvq gqnslnltsd kpsvqrkeqn hskhsiqskl
       61 trlyfeeltk vphatpdsvl nnlenecdll grlvqreglh tlivnlyagn kgyslavrns
      121 dkgnqydkns ilaetqlmgy eqgellscid ngqlpamlae qletnhshlf ydgciiaevr
      181 dyrktfpqtk aevhhvllkp ttqsvlsdvs tltsdgdwsh eerlmleshl vaatqgplcl
      241 dpnpipslat trlrqsksll tdhqlirqak kfsqvtvnrk rkleqlaqpe gltiqdlmqk
      301 lkakrgvvtt tacpppsltn ppllppntpi dvlrfakaye rpretkdclp qvieeyilet
      361 ggsqgeidhi klsilqrpsn seylgelymd knhregekng sacrftlgtr vlanhyiqqf
      421 teifteegrk nvrikhvvpg qvprvtctpg mqrahqqaqq akaaqlvaqh lqqaqsqhva
      481 qqilsraqgq lntlagqvas iktiteatts aqnnltcvds snipqviaqs dasslspgqs
      541 ltnggsnast svqidnstms vtdgsscngs gilvfqqkst nninnatstn vpvlqaqlqa
      601 gtqncvtetv nqnqneipfk khstnpaita lvtslmnsaq qfqqqaaana aaaamnsntq
      661 tpnksnnaai lsllnsspan ltqqkvqprr islnasipsr vlsshgnvin vpnttgqvrv
      721 slsstltgql gckqqpvkat avrlarvqdp tstlglsmpg lsallagtps adnpipgins
      781 gssllerlta sssqnnqaat smttppstnv nlqgvnltsl snsinglqnv qvsfpglsqp
      841 itmslnvsat tgsvtptgvi vslpissatn tcttvssmva ttvvttaiag stptvvianp
      901 anthlslpia qiipsgvkgl sqqnirssns vtlsqggqai qlvgsqrprl nhmtrqvqpn
      961 qvkstvlpnq lvtvaktvta sqlilsgnkq vltpimatkp vlmasqttps sqvvpankqt
     1021 lltkslhara stghcsqqtq slgvatlsqq qlqrtlvkpv qlqqlqclaa qhkvavatgn
     1081 aqnrtqiesq rtststpged pa
//
LOCUS       XP_034194927            1101 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta 1, catalytic subunit isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194927
VERSION     XP_034194927.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339036.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1101
                     /product="DNA polymerase delta 1, catalytic subunit
                     isoform X1"
                     /calculated_mol_wt=125705
     Region          77..1097
                     /region_name="PTZ00166"
                     /note="DNA polymerase delta catalytic subunit;
                     Provisional"
                     /db_xref="CDD:240301"
     CDS             1..1101
                     /gene="PolD1"
                     /coded_by="XM_034339036.2:121..3426"
                     /db_xref="GeneID:117611119"
ORIGIN      
        1 mnrkkfstps aakkakygdd ddddlpgsfe aelatmdete ddfgdvsqfv eepadvklnd
       61 klfrgpeqen issrwsrppp pdlnpeedal ifqqieidhy ngaplvgmpg stippvpimr
      121 mygiteqgns vcchvhgfsp ylyvtappkf tdshcnrfkd aldkailkdm rsnteniqia
      181 vlavervykq tmygytgdek slflkitvtl pkliapcrrl lekenifpef vhtyrdfesn
      241 idfdvrfmvd tsivgcswie lpskvwklrg rhghdlpsit rcqieadvaw dafishspee
      301 wskiapfril sfdiecagrk gifpepnhdp viqianmvik qgdsepflrn iftlntcapi
      361 igcqvlsfdk eqtllekwae fvrqldpdif tgyninnfdf pylinraqhl nvknfnflgr
      421 ikniksvvkt kiiqskqmgr renksvnfeg rvpfdlylvl vrdyklrsyt lnavsyhflq
      481 eqkedvhhsi itelqngnsq trrrlavycl kdaylplrll dklmcifiym emarvtgvsi
      541 aslltrgqqi kvvsqllrki rekdylmpgy qsqgsedqfe gatviepkrg yyndpiatld
      601 fsslypsiim ahnlcyttll nrewqtkhei dpdditktps nsmfikanvk kgilpeilen
      661 llsarktaka qlknetdplk qkvldgrqya lkvsansvyg ftgaqvgklp cleisasvta
      721 ygramiertk qevedhycts ngyennavvi ygdtdsvmvk fgvktikeam algkeaaeyv
      781 sekflkpikl efekvyfpyl linkkryagl yftcpekydk mdckgletvr rdncplvanm
      841 mntclqklli drnptdalny akqvisdllc nridisqlvi tkeltktdyt akqahvelaa
      901 kmkkrdtgta pklgdrvpyv fitaakgapa yqkaedpiyv lennipidtn yylenqlskp
      961 lvrifepilg dkaeslllkg dhtrtksvat srvgalsaft rkkevclgck avlaperekm
     1021 alcthceske aevfqtelyv grkleekfcr lwtecqrcqg slhqevicts rdcpifymrk
     1081 kvqmeldtqi krierfgtpq w
//
LOCUS       XP_034194931            1089 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase delta 1, catalytic subunit isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194931
VERSION     XP_034194931.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339040.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1089
                     /product="DNA polymerase delta 1, catalytic subunit
                     isoform X2"
                     /calculated_mol_wt=124308
     Region          37..1085
                     /region_name="PTZ00166"
                     /note="DNA polymerase delta catalytic subunit;
                     Provisional"
                     /db_xref="CDD:240301"
     CDS             1..1089
                     /gene="PolD1"
                     /coded_by="XM_034339040.2:121..3390"
                     /db_xref="GeneID:117611119"
ORIGIN      
        1 mnrkkfstps aakkakygdd ddddlpgsfe aelatmdete ddfgdvsqfv eegpeqenis
       61 srwsrppppd lnpeedalif qqieidhyng aplvgmpgst ippvpimrmy giteqgnsvc
      121 chvhgfspyl yvtappkftd shcnrfkdal dkailkdmrs nteniqiavl avervykqtm
      181 ygytgdeksl flkitvtlpk liapcrrlle kenifpefvh tyrdfesnid fdvrfmvdts
      241 ivgcswielp skvwklrgrh ghdlpsitrc qieadvawda fishspeews kiapfrilsf
      301 diecagrkgi fpepnhdpvi qianmvikqg dsepflrnif tlntcapiig cqvlsfdkeq
      361 tllekwaefv rqldpdiftg yninnfdfpy linraqhlnv knfnflgrik niksvvktki
      421 iqskqmgrre nksvnfegrv pfdlylvlvr dyklrsytln avsyhflqeq kedvhhsiit
      481 elqngnsqtr rrlavyclkd aylplrlldk lmcifiymem arvtgvsias lltrgqqikv
      541 vsqllrkire kdylmpgyqs qgsedqfega tviepkrgyy ndpiatldfs slypsiimah
      601 nlcyttllnr ewqtkheidp dditktpsns mfikanvkkg ilpeilenll sarktakaql
      661 knetdplkqk vldgrqyalk vsansvygft gaqvgklpcl eisasvtayg ramiertkqe
      721 vedhyctsng yennavviyg dtdsvmvkfg vktikeamal gkeaaeyvse kflkpiklef
      781 ekvyfpylli nkkryaglyf tcpekydkmd ckgletvrrd ncplvanmmn tclqkllidr
      841 nptdalnyak qvisdllcnr idisqlvitk eltktdytak qahvelaakm kkrdtgtapk
      901 lgdrvpyvfi taakgapayq kaedpiyvle nnipidtnyy lenqlskplv rifepilgdk
      961 aeslllkgdh trtksvatsr vgalsaftrk kevclgckav laperekmal cthceskeae
     1021 vfqtelyvgr kleekfcrlw tecqrcqgsl hqevictsrd cpifymrkkv qmeldtqikr
     1081 ierfgtpqw
//
LOCUS       XP_034194946            1014 aa            linear   INV 03-SEP-2025
DEFINITION  RNA cytidine acetyltransferase l(1)G0020 [Osmia lignaria lignaria].
ACCESSION   XP_034194946
VERSION     XP_034194946.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339055.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194946.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1014
                     /product="RNA cytidine acetyltransferase l(1)G0020"
                     /calculated_mol_wt=115300
     Region          9..201
                     /region_name="TmcA_N"
                     /note="tRNA(Met) cytidine acetyltransferase TmcA,
                     N-terminal; pfam08351"
                     /db_xref="CDD:462441"
     Region          107..897
                     /region_name="TmcA"
                     /note="tRNA(Met) C34 N-acetyltransferase TmcA
                     [Translation, ribosomal structure and biogenesis];
                     COG1444"
                     /db_xref="CDD:441053"
     Region          521..746
                     /region_name="GNAT_acetyltr_2"
                     /note="GNAT acetyltransferase 2; pfam13718"
                     /db_xref="CDD:463966"
     CDS             1..1014
                     /gene="l(1)G0020"
                     /coded_by="XM_034339055.2:271..3315"
                     /db_xref="GeneID:117611130"
ORIGIN      
        1 mvrkkidnri rvliengvnt ghrtlfivig ekardqivll hhmlsksvvk arpsvlwcyk
       61 kdlgfsshrk krmksiqkkv ksgkldvned dpfelfvvst nirycyyhet hkilgntygm
      121 cvlqdfeait pnllartiet ieggglivfl lqsvnslkql ytmnmdvhqr frteahqnvv
      181 grfnerflls lasckrclvv ddqlnvlpis shnlkiepvq kltsseeqse ldilkeslkd
      241 tqpvsslvnc cktidqakal lkfiecisek tlrstvslta argrgksaal glaiagaitf
      301 gysniyvssp spenlntlfe fvfkgfdglg yqehldyglv qstnpefnka tvrvnvfrdh
      361 rqtiqyihpt dayklnqael lvideaaaip lpyvkamlgp yliflsstin gyegtgrsls
      421 lkllqqlrsq tvgsnsheks ekqqnektvi grqlheltle esirykpgds ieqwlcdllc
      481 lnathtlils gcpppdmcql yyinrdtlfs yhkaselflq rlvalyvash yknspndlqm
      541 msdapahhlf cllgpidsnk kslpeilavi qiclegemsk navndglvrg rkaagdlipw
      601 amaqqyqdqd fpmlagariv riathpdyhg mgygsralel lkqyyemkil niseapletc
      661 nteiskvede kvnlleeqie praslpplll klterrpenl dyigvsfgvt eqlmkfwkra
      721 nfvpvylrqt pnditgeysc imlyklnfeq edskwlqayw ndfrrrfinl lsfsfnsyps
      781 slalsilink tvkpetttlt keildvyfts ydlkrlsmys nnmadyhlim dllpslahly
      841 flnmmsdthl savqsaillg lglqhktvdk laeeldlpss qllalfnrii rkcvqylngi
      901 aenfiestmm kiqtsnedvk lnpingkslh deletaakel kakqkaelek lkrenleqya
      961 ikgsetewsn vlsgkkaknl isiksgekri seddsalerp kkqykkkirr sfkt
//
LOCUS       XP_034194959             998 aa            linear   INV 03-SEP-2025
DEFINITION  proton channel OtopLc isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194959
VERSION     XP_034194959.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339068.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194959.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..998
                     /product="proton channel OtopLc isoform X1"
                     /calculated_mol_wt=111509
     Region          514..974
                     /region_name="Otopetrin"
                     /note="pfam03189"
                     /db_xref="CDD:460840"
     CDS             1..998
                     /gene="LOC117611139"
                     /coded_by="XM_034339068.2:1..2997"
                     /db_xref="GeneID:117611139"
ORIGIN      
        1 mvaeltdipi asverktptv dpgklrssar rrnrprwiqt atrepiysvl dsnervvprn
       61 dlpvgvkrkv vvisavksdk rgdkptaeev ededplysvv rkpkkvrlps etggfferrs
      121 lqpikegsse ektcsrqeel rkwlqiaskq lpeprrlfth gemytnhpgd hdqlprsvsm
      181 prgivglvgp yrvyqnpner reymlqeeel veevvdsvse iaarendtke hrqrakwrir
      241 eedeetlvpd irdipgleef tgevdnkets ewkqvddqst ieynkteeis kskdeskgsf
      301 seiltgkeer aidvpddtst istslvkekk asneqndkds lnetgsttgg tvegapggap
      361 nrgernpete gttvettrts vpsrslvsri lsktrstndl ldhsepfsql wivlsnvygq
      421 liivmmialc laevmdtpvp llslqgvflm ylyvgsiavi isiyiwvlvd scgslgtgaa
      481 gvddaelgga sltrfgslkr ahisrsrtap tsfyirvgal lfglatlvfn glemamhsmm
      541 qgaeclsdvv fvhpvlhglf tflqmhflfv nsqvlverfg laarfgfihl aatniavwvr
      601 lviwdsaqew tyfvhlaqrg lhnsvsplnl rgfpeslirh trdligsfpf qlltayifnk
      661 lyvyisdhss sekdvyqpyq pisneqidqv ialqeclntn tlgqlwtssm pflypfivqf
      721 sliaaavtfv mgqnvgrsrl vhkqkfhgsk dlashtkvgc dgsskglflg ilcmvagivv
      781 iliflvvred ehfpsatlsw ltcgtltsil tlstlmtasg lvqvrqmsvv srapaaldsl
      841 lsnvalfgvq lysiftivvs acslalledv snetrgryim llaasilqlv qsfaqstlia
      901 easkrscitr fqiiakparq vitfllfsns vlwafdtvit qnwisqelql rffgvlawgv
      961 isriglplli fyrfhscvll leawnkcyrm prgehpln
//
LOCUS       XP_034194965             977 aa            linear   INV 03-SEP-2025
DEFINITION  proton channel OtopLc isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194965
VERSION     XP_034194965.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339074.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194965.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..977
                     /product="proton channel OtopLc isoform X2"
                     /calculated_mol_wt=109042
     Region          514..953
                     /region_name="Otopetrin"
                     /note="pfam03189"
                     /db_xref="CDD:460840"
     CDS             1..977
                     /gene="LOC117611139"
                     /coded_by="XM_034339074.2:1..2934"
                     /db_xref="GeneID:117611139"
ORIGIN      
        1 mvaeltdipi asverktptv dpgklrssar rrnrprwiqt atrepiysvl dsnervvprn
       61 dlpvgvkrkv vvisavksdk rgdkptaeev ededplysvv rkpkkvrlps etggfferrs
      121 lqpikegsse ektcsrqeel rkwlqiaskq lpeprrlfth gemytnhpgd hdqlprsvsm
      181 prgivglvgp yrvyqnpner reymlqeeel veevvdsvse iaarendtke hrqrakwrir
      241 eedeetlvpd irdipgleef tgevdnkets ewkqvddqst ieynkteeis kskdeskgsf
      301 seiltgkeer aidvpddtst istslvkekk asneqndkds lnetgsttgg tvegapggap
      361 nrgernpete gttvettrts vpsrslvsri lsktrstndl ldhsepfsql wivlsnvygq
      421 liivmmialc laevmdtpvp llslqgvflm ylyvgsiavi isiyiwvlvd scgslgtgaa
      481 gvddaelgga sltrfgslkr ahisrsrtap tsfyirvgal lfglatlvfn glemamhsmm
      541 qgaeclsdvv fvhpvlhglf tflqmhflfv nsqvlverfg laarfgfihl aatniavwvr
      601 lviwdsaqew tyfvhlaqrg lhnsvsplnl rgfpeslirh trdlidhsss ekdvyqpyqp
      661 isneqidqvi alqeclntnt lgqlwtssmp flypfivqfs liaaavtfvm gqnvgrsrlv
      721 hkqkfhgskd lashtkvgcd gsskglflgi lcmvagivvi liflvvrede hfpsatlswl
      781 tcgtltsilt lstlmtasgl vqvrqmsvvs rapaaldsll snvalfgvql ysiftivvsa
      841 cslalledvs netrgryiml laasilqlvq sfaqstliae askrscitrf qiiakparqv
      901 itfllfsnsv lwafdtvitq nwisqelqlr ffgvlawgvi sriglpllif yrfhscvlll
      961 eawnkcyrmp rgehpln
//
LOCUS       XP_034194977             869 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194977
VERSION     XP_034194977.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339086.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="N-acetyltransferase 1 isoform X1"
                     /calculated_mol_wt=99494
     Region          37..265
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          355..460
                     /region_name="DUF2756"
                     /note="Protein of unknown function (DUF2756); cl08110"
                     /db_xref="CDD:415463"
     Region          497..604
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          679..824
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..869
                     /gene="NAT1"
                     /coded_by="XM_034339086.2:348..2957"
                     /db_xref="GeneID:117611151"
ORIGIN      
        1 mpsrddirsl steqrwipps tvrrdaltpe srndlifrkv rgilnkltpe kfaklsndll
       61 nvelnsdvil kgviflifek aldepkyssm yaqlckrlsd eaanfeprka lidsqkgqst
      121 ftllllnkck defenrskas eafenqdelg peeeerrqva krkmlgnikf igelgklgiv
      181 sepilhrciq qllekkrrgg srgdtaedie clcqimrtcg rildsdkgrg lmdqyfrrmn
      241 slaessdlpl rikfmlrdvi elrrdgwvpr katstegpmp inqirndnee ssrgngfhrr
      301 edrlgaeflr kmgrsgldmd mmgsipltsp sfgmpppfsp ngfsgtsgvg ygrhnqrnqp
      361 gyyqnqnrhq nnyqgkhnqq qhnspqnfnn nsskeqlrfn knkmlighpe evslrpsans
      421 mmfkqtninp nlplnsdlfp graselpllr tntlkpsspl lhkelppaiv ikqgpvdkre
      481 kardrkdkgt sreeilkkvn almddlvsht niqdaitafq dlkiperflr havytlysnt
      541 ldrgdgerel vaklivelvk advitgqqvh egwkelvsni aekestvpcv ashvafltak
      601 aivdnliqit dlvavtengq hhplflltlq qlhksqgkar ltqifneskv nlisqlpead
      661 ktkerlaeil edreltflyp llkiqgdiwr qleydpnpnt lykwikekle pshhsnsefi
      721 nalmnvllky itqetilvpg vdpsnvaksv idkekallek ysrlmrlffv diesqvtalh
      781 alqafwfshn fpkgmllrwf dglyrlevie edayfkwkec vtdaypgkgk alfevnswlt
      841 wldnastede sddhndddds nknyrmqkl
//
LOCUS       XP_034194983             869 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194983
VERSION     XP_034194983.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339092.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="N-acetyltransferase 1 isoform X1"
                     /calculated_mol_wt=99494
     Region          37..265
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          355..460
                     /region_name="DUF2756"
                     /note="Protein of unknown function (DUF2756); cl08110"
                     /db_xref="CDD:415463"
     Region          497..604
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          679..824
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..869
                     /gene="NAT1"
                     /coded_by="XM_034339092.2:455..3064"
                     /db_xref="GeneID:117611151"
ORIGIN      
        1 mpsrddirsl steqrwipps tvrrdaltpe srndlifrkv rgilnkltpe kfaklsndll
       61 nvelnsdvil kgviflifek aldepkyssm yaqlckrlsd eaanfeprka lidsqkgqst
      121 ftllllnkck defenrskas eafenqdelg peeeerrqva krkmlgnikf igelgklgiv
      181 sepilhrciq qllekkrrgg srgdtaedie clcqimrtcg rildsdkgrg lmdqyfrrmn
      241 slaessdlpl rikfmlrdvi elrrdgwvpr katstegpmp inqirndnee ssrgngfhrr
      301 edrlgaeflr kmgrsgldmd mmgsipltsp sfgmpppfsp ngfsgtsgvg ygrhnqrnqp
      361 gyyqnqnrhq nnyqgkhnqq qhnspqnfnn nsskeqlrfn knkmlighpe evslrpsans
      421 mmfkqtninp nlplnsdlfp graselpllr tntlkpsspl lhkelppaiv ikqgpvdkre
      481 kardrkdkgt sreeilkkvn almddlvsht niqdaitafq dlkiperflr havytlysnt
      541 ldrgdgerel vaklivelvk advitgqqvh egwkelvsni aekestvpcv ashvafltak
      601 aivdnliqit dlvavtengq hhplflltlq qlhksqgkar ltqifneskv nlisqlpead
      661 ktkerlaeil edreltflyp llkiqgdiwr qleydpnpnt lykwikekle pshhsnsefi
      721 nalmnvllky itqetilvpg vdpsnvaksv idkekallek ysrlmrlffv diesqvtalh
      781 alqafwfshn fpkgmllrwf dglyrlevie edayfkwkec vtdaypgkgk alfevnswlt
      841 wldnastede sddhndddds nknyrmqkl
//
LOCUS       XP_034194990             869 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194990
VERSION     XP_034194990.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339099.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="N-acetyltransferase 1 isoform X1"
                     /calculated_mol_wt=99494
     Region          37..265
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          355..460
                     /region_name="DUF2756"
                     /note="Protein of unknown function (DUF2756); cl08110"
                     /db_xref="CDD:415463"
     Region          497..604
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          679..824
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..869
                     /gene="NAT1"
                     /coded_by="XM_034339099.2:281..2890"
                     /db_xref="GeneID:117611151"
ORIGIN      
        1 mpsrddirsl steqrwipps tvrrdaltpe srndlifrkv rgilnkltpe kfaklsndll
       61 nvelnsdvil kgviflifek aldepkyssm yaqlckrlsd eaanfeprka lidsqkgqst
      121 ftllllnkck defenrskas eafenqdelg peeeerrqva krkmlgnikf igelgklgiv
      181 sepilhrciq qllekkrrgg srgdtaedie clcqimrtcg rildsdkgrg lmdqyfrrmn
      241 slaessdlpl rikfmlrdvi elrrdgwvpr katstegpmp inqirndnee ssrgngfhrr
      301 edrlgaeflr kmgrsgldmd mmgsipltsp sfgmpppfsp ngfsgtsgvg ygrhnqrnqp
      361 gyyqnqnrhq nnyqgkhnqq qhnspqnfnn nsskeqlrfn knkmlighpe evslrpsans
      421 mmfkqtninp nlplnsdlfp graselpllr tntlkpsspl lhkelppaiv ikqgpvdkre
      481 kardrkdkgt sreeilkkvn almddlvsht niqdaitafq dlkiperflr havytlysnt
      541 ldrgdgerel vaklivelvk advitgqqvh egwkelvsni aekestvpcv ashvafltak
      601 aivdnliqit dlvavtengq hhplflltlq qlhksqgkar ltqifneskv nlisqlpead
      661 ktkerlaeil edreltflyp llkiqgdiwr qleydpnpnt lykwikekle pshhsnsefi
      721 nalmnvllky itqetilvpg vdpsnvaksv idkekallek ysrlmrlffv diesqvtalh
      781 alqafwfshn fpkgmllrwf dglyrlevie edayfkwkec vtdaypgkgk alfevnswlt
      841 wldnastede sddhndddds nknyrmqkl
//
LOCUS       XP_034194994             865 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194994
VERSION     XP_034194994.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339103.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..865
                     /product="N-acetyltransferase 1 isoform X2"
                     /calculated_mol_wt=99021
     Region          37..265
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          493..600
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          675..820
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..865
                     /gene="NAT1"
                     /coded_by="XM_034339103.2:349..2946"
                     /db_xref="GeneID:117611151"
ORIGIN      
        1 mpsrddirsl steqrwipps tvrrdaltpe srndlifrkv rgilnkltpe kfaklsndll
       61 nvelnsdvil kgviflifek aldepkyssm yaqlckrlsd eaanfeprka lidsqkgqst
      121 ftllllnkck defenrskas eafenqdelg peeeerrqva krkmlgnikf igelgklgiv
      181 sepilhrciq qllekkrrgg srgdtaedie clcqimrtcg rildsdkgrg lmdqyfrrmn
      241 slaessdlpl rikfmlrdvi elrrdgwvpr katstegpmp inqirndnee ssrgngfhrr
      301 edrlgaeflr kmgrsgldmd mmgsipltsp sfgmpppfsp ngfsgtsgvg ygrhnqrnqp
      361 gyyqnqnrhq nnyqgkhnqq qhnspqnfnn nsskeqlrfn knkmlighpe evslrpsans
      421 mmfkqtninp nlplnsgras elpllrtntl kpsspllhke lppaivikqg pvdkrekard
      481 rkdkgtsree ilkkvnalmd dlvshtniqd aitafqdlki perflrhavy tlysntldrg
      541 dgerelvakl ivelvkadvi tgqqvhegwk elvsniaeke stvpcvashv afltakaivd
      601 nliqitdlva vtengqhhpl flltlqqlhk sqgkarltqi fneskvnlis qlpeadktke
      661 rlaeiledre ltflypllki qgdiwrqley dpnpntlykw ikeklepshh snsefinalm
      721 nvllkyitqe tilvpgvdps nvaksvidke kallekysrl mrlffvdies qvtalhalqa
      781 fwfshnfpkg mllrwfdgly rlevieeday fkwkecvtda ypgkgkalfe vnswltwldn
      841 astedesddh nddddsnkny rmqkl
//
LOCUS       XP_034194998             780 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034194998
VERSION     XP_034194998.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339107.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..780
                     /product="N-acetyltransferase 1 isoform X3"
                     /calculated_mol_wt=89255
     Region          <1..176
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          266..371
                     /region_name="DUF2756"
                     /note="Protein of unknown function (DUF2756); cl08110"
                     /db_xref="CDD:415463"
     Region          408..515
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          590..735
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..780
                     /gene="NAT1"
                     /coded_by="XM_034339107.2:486..2828"
                     /db_xref="GeneID:117611151"
ORIGIN      
        1 myaqlckrls deaanfeprk alidsqkgqs tftllllnkc kdefenrska seafenqdel
       61 gpeeeerrqv akrkmlgnik figelgklgi vsepilhrci qqllekkrrg gsrgdtaedi
      121 eclcqimrtc grildsdkgr glmdqyfrrm nslaessdlp lrikfmlrdv ielrrdgwvp
      181 rkatstegpm pinqirndne essrgngfhr redrlgaefl rkmgrsgldm dmmgsiplts
      241 psfgmpppfs pngfsgtsgv gygrhnqrnq pgyyqnqnrh qnnyqgkhnq qqhnspqnfn
      301 nnsskeqlrf nknkmlighp eevslrpsan smmfkqtnin pnlplnsdlf pgraselpll
      361 rtntlkpssp llhkelppai vikqgpvdkr ekardrkdkg tsreeilkkv nalmddlvsh
      421 tniqdaitaf qdlkiperfl rhavytlysn tldrgdgere lvaklivelv kadvitgqqv
      481 hegwkelvsn iaekestvpc vashvaflta kaivdnliqi tdlvavteng qhhplflltl
      541 qqlhksqgka rltqifnesk vnlisqlpea dktkerlaei ledreltfly pllkiqgdiw
      601 rqleydpnpn tlykwikekl epshhsnsef inalmnvllk yitqetilvp gvdpsnvaks
      661 vidkekalle kysrlmrlff vdiesqvtal halqafwfsh nfpkgmllrw fdglyrlevi
      721 eedayfkwke cvtdaypgkg kalfevnswl twldnasted esddhndddd snknyrmqkl
//
LOCUS       XP_034195006             780 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195006
VERSION     XP_034195006.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339115.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..780
                     /product="N-acetyltransferase 1 isoform X3"
                     /calculated_mol_wt=89255
     Region          <1..176
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          266..371
                     /region_name="DUF2756"
                     /note="Protein of unknown function (DUF2756); cl08110"
                     /db_xref="CDD:415463"
     Region          408..515
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          590..735
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..780
                     /gene="NAT1"
                     /coded_by="XM_034339115.2:395..2737"
                     /db_xref="GeneID:117611151"
ORIGIN      
        1 myaqlckrls deaanfeprk alidsqkgqs tftllllnkc kdefenrska seafenqdel
       61 gpeeeerrqv akrkmlgnik figelgklgi vsepilhrci qqllekkrrg gsrgdtaedi
      121 eclcqimrtc grildsdkgr glmdqyfrrm nslaessdlp lrikfmlrdv ielrrdgwvp
      181 rkatstegpm pinqirndne essrgngfhr redrlgaefl rkmgrsgldm dmmgsiplts
      241 psfgmpppfs pngfsgtsgv gygrhnqrnq pgyyqnqnrh qnnyqgkhnq qqhnspqnfn
      301 nnsskeqlrf nknkmlighp eevslrpsan smmfkqtnin pnlplnsdlf pgraselpll
      361 rtntlkpssp llhkelppai vikqgpvdkr ekardrkdkg tsreeilkkv nalmddlvsh
      421 tniqdaitaf qdlkiperfl rhavytlysn tldrgdgere lvaklivelv kadvitgqqv
      481 hegwkelvsn iaekestvpc vashvaflta kaivdnliqi tdlvavteng qhhplflltl
      541 qqlhksqgka rltqifnesk vnlisqlpea dktkerlaei ledreltfly pllkiqgdiw
      601 rqleydpnpn tlykwikekl epshhsnsef inalmnvllk yitqetilvp gvdpsnvaks
      661 vidkekalle kysrlmrlff vdiesqvtal halqafwfsh nfpkgmllrw fdglyrlevi
      721 eedayfkwke cvtdaypgkg kalfevnswl twldnasted esddhndddd snknyrmqkl
//
LOCUS       XP_034195010             780 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetyltransferase 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195010
VERSION     XP_034195010.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339119.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..780
                     /product="N-acetyltransferase 1 isoform X3"
                     /calculated_mol_wt=89255
     Region          <1..176
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          266..371
                     /region_name="DUF2756"
                     /note="Protein of unknown function (DUF2756); cl08110"
                     /db_xref="CDD:415463"
     Region          408..515
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          590..735
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..780
                     /gene="NAT1"
                     /coded_by="XM_034339119.2:593..2935"
                     /db_xref="GeneID:117611151"
ORIGIN      
        1 myaqlckrls deaanfeprk alidsqkgqs tftllllnkc kdefenrska seafenqdel
       61 gpeeeerrqv akrkmlgnik figelgklgi vsepilhrci qqllekkrrg gsrgdtaedi
      121 eclcqimrtc grildsdkgr glmdqyfrrm nslaessdlp lrikfmlrdv ielrrdgwvp
      181 rkatstegpm pinqirndne essrgngfhr redrlgaefl rkmgrsgldm dmmgsiplts
      241 psfgmpppfs pngfsgtsgv gygrhnqrnq pgyyqnqnrh qnnyqgkhnq qqhnspqnfn
      301 nnsskeqlrf nknkmlighp eevslrpsan smmfkqtnin pnlplnsdlf pgraselpll
      361 rtntlkpssp llhkelppai vikqgpvdkr ekardrkdkg tsreeilkkv nalmddlvsh
      421 tniqdaitaf qdlkiperfl rhavytlysn tldrgdgere lvaklivelv kadvitgqqv
      481 hegwkelvsn iaekestvpc vashvaflta kaivdnliqi tdlvavteng qhhplflltl
      541 qqlhksqgka rltqifnesk vnlisqlpea dktkerlaei ledreltfly pllkiqgdiw
      601 rqleydpnpn tlykwikekl epshhsnsef inalmnvllk yitqetilvp gvdpsnvaks
      661 vidkekalle kysrlmrlff vdiesqvtal halqafwfsh nfpkgmllrw fdglyrlevi
      721 eedayfkwke cvtdaypgkg kalfevnswl twldnasted esddhndddd snknyrmqkl
//
LOCUS       XP_034195015             846 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611187 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195015
VERSION     XP_034195015.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339124.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195015.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..846
                     /product="uncharacterized protein LOC117611187 isoform X1"
                     /calculated_mol_wt=95063
     Region          <57..268
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          <96..>442
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          461..719
                     /region_name="EHD"
                     /note="Eps15 homology domain (EHD), C-terminal domain;
                     cd09913"
                     /db_xref="CDD:206740"
     Site            466..473
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206740"
     Site            order(471..474,573,639,641,675..677)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206740"
     Site            516
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206740"
     Site            520..522
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206740"
     Site            573..576
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206740"
     Site            order(575..576,602..603)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206740"
     Site            638..641
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206740"
     Site            675..677
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206740"
     CDS             1..846
                     /gene="LOC117611187"
                     /coded_by="XM_034339124.2:632..3172"
                     /db_xref="GeneID:117611187"
ORIGIN      
        1 mtgsrlrpay fvglflallv valqgvqsee elevppedlc rpyiekalkh lgtgsleqts
       61 teagtdadks heegeegdgk paseydaepg tdgsredvat eeekqesvea egadsteqge
      121 eesatasaea eqsaeadete egeaekaeeq saeadegtee ekesvenveg aadseqdgse
      181 eekksgeepq vdeeaqtedg ksdeegdeqs aeeakldeek sdeakteeee tdskaeetee
      241 vtdaaskedd tedskaeeet qsqeqplsee eegkegsegq eesaegqvek eeeeeevkes
      301 eeavkstedv ksaeeagees ksgeeegksr eeaksdeeea kldeeeaksr arrevgeeda
      361 deasketaes eeeeeptpee dliqeievpe nlrprehinq llkldeened keraiekiad
      421 vilkdlkkvy dnaiqplesl ykyrdlsnrh fgdpeifskp lilfmgpwsg gkssiinyll
      481 dieykqtslr tgglrkclee qcktangndt gaepspayfn ilmhgdeeei ldgtqlaadw
      541 tfsglqkfgq glldrlkglr lddkllkkvn iveipgilei rkqvqrlfpf ndacqwfidr
      601 adiiflvydp skldvgpete aildqlkgre hqtriilnka dqvkpeelmr vqgaliwnis
      661 plmssaeppv mysaslwslp yeagaptrll yaqeraflrd lrtaidkrve hkiasarrfa
      721 vrvrnhakmv dcylttyynh ktffankkei sdkiienpqd yhiyeglstl knisrydlpd
      781 pdvyrdffrl nplydfplls stctyfrgcp inrldvaiay dlpelvgkyr ksveqvihey
      841 etnpss
//
LOCUS       XP_034195021             827 aa            linear   INV 03-SEP-2025
DEFINITION  sarcalumenin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195021
VERSION     XP_034195021.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339130.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195021.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..827
                     /product="sarcalumenin isoform X2"
                     /calculated_mol_wt=93044
     Region          <57..268
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          <96..>442
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          461..700
                     /region_name="EHD"
                     /note="Eps15 homology domain (EHD), C-terminal domain;
                     cd09913"
                     /db_xref="CDD:206740"
     Site            466..473
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206740"
     Site            order(471..474,554,620,622,656..658)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206740"
     Site            497
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206740"
     Site            501..503
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206740"
     Site            554..557
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206740"
     Site            order(556..557,583..584)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206740"
     Site            619..622
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206740"
     Site            656..658
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206740"
     CDS             1..827
                     /gene="LOC117611187"
                     /coded_by="XM_034339130.2:627..3110"
                     /db_xref="GeneID:117611187"
ORIGIN      
        1 mtgsrlrpay fvglflallv valqgvqsee elevppedlc rpyiekalkh lgtgsleqts
       61 teagtdadks heegeegdgk paseydaepg tdgsredvat eeekqesvea egadsteqge
      121 eesatasaea eqsaeadete egeaekaeeq saeadegtee ekesvenveg aadseqdgse
      181 eekksgeepq vdeeaqtedg ksdeegdeqs aeeakldeek sdeakteeee tdskaeetee
      241 vtdaaskedd tedskaeeet qsqeqplsee eegkegsegq eesaegqvek eeeeeevkes
      301 eeavkstedv ksaeeagees ksgeeegksr eeaksdeeea kldeeeaksr arrevgeeda
      361 deasketaes eeeeeptpee dliqeievpe nlrprehinq llkldeened keraiekiad
      421 vilkdlkkvy dnaiqplesl ykyrdlsnrh fgdpeifskp lilfmgpwsg gkssiinyll
      481 dieykqtslr tgaepspayf nilmhgdeee ildgtqlaad wtfsglqkfg qglldrlkgl
      541 rlddkllkkv niveipgile irkqvqrlfp fndacqwfid radiiflvyd pskldvgpet
      601 eaildqlkgr ehqtriilnk adqvkpeelm rvqgaliwni splmssaepp vmysaslwsl
      661 pyeagaptrl lyaqeraflr dlrtaidkrv ehkiasarrf avrvrnhakm vdcylttyyn
      721 hktffankke isdkiienpq dyhiyeglst lknisrydlp dpdvyrdffr lnplydfpll
      781 sstctyfrgc pinrldvaia ydlpelvgky rksveqvihe yetnpss
//
LOCUS       XP_034195463             819 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase regulator associated with FAK isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195463
VERSION     XP_034195463.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339572.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..819
                     /product="GTPase regulator associated with FAK isoform X1"
                     /calculated_mol_wt=93063
     Region          21..227
                     /region_name="BAR_RhoGAP_OPHN1-like"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain of
                     Oligophrenin1-like Rho GTPase Activating Proteins;
                     cd07602"
                     /db_xref="CDD:153286"
     Site            order(30,33..34,38,40..41,44..45,47..48,51..52,54..55,
                     58..59,61..62,65,92,95..96,188,191..192,194,199,202..203,
                     205..206,210..211,214..215,217..218,220..221,223..224)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153286"
     Region          272..376
                     /region_name="BAR-PH_GRAF_family"
                     /note="GTPase Regulator Associated with Focal adhesion and
                     related proteins Pleckstrin homology (PH) domain; cd01249"
                     /db_xref="CDD:269953"
     Region          369..572
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(421,463,467,536,539..540,563)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238049"
     Site            421
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238049"
     Region          764..816
                     /region_name="SH3_GRAF-like"
                     /note="Src Homology 3 domain of GTPase Regulator
                     Associated with Focal adhesion kinase and similar
                     proteins; cd11882"
                     /db_xref="CDD:212815"
     Site            order(769,771,774,778,797..798,811,813..814)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212815"
     CDS             1..819
                     /gene="Graf"
                     /coded_by="XM_034339572.2:268..2727"
                     /db_xref="GeneID:117611586"
ORIGIN      
        1 mgvglkplef tdcltdspyf renlhyhere lentnqqikr likevkglll aaknlskaqk
       61 afsktlqnfs fecigetqte deshisqslk kfseliasie derdrmlkra ddqiilplen
      121 frkehiggvk egkkkfekqt ikfcqsqery lnlstkkqdn vlqeadatle maerhfcqas
      181 leyvfllqev qerkkfefve tllgfmfgwl tfyhqgheva kdfkpymtel qlkiqktrdn
      241 flatrdktes lmnkmkdmkk savesgvnkl ytregylflm ekkafgttwt kqyctyqkdk
      301 keftmipynq ltgkfsskev ltlascirrm sdtiekrfcf dittvdkpnv vytfqalsee
      361 drklwldamd gkesvsikny aavgastkse envldetgfm fvskciaale ergleeqgly
      421 rvvgvaskvn kllamgldrr kleklnledr fewesktits alktylrtls eplmtfryyn
      481 sfisaakqev kearisdihn lvyrlpkanf nmlvllirhl csvakksdkn lmtvgnlavc
      541 fgpsllrpee etvasimdik fynivveili enceriiagp pqdtirstqn tapprsqrvn
      601 tedgsassgn gtpflkypvh vmssphghhi trpyydgpsa vvtktpsidd rkngdyeeve
      661 htshrippyl dsrsgrvkln nanvmyhsad kvltsgntss ssesiapdph sfssdlpvkq
      721 krgsmmpvhy psgytqrnnp pvsvintspg sgrsspgqvp giwrvrtlya cvaesdgels
      781 fepnqiitnv kaslepgwle gtlngktglv pknyveqlp
//
LOCUS       XP_034195464             819 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase regulator associated with FAK isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195464
VERSION     XP_034195464.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339573.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..819
                     /product="GTPase regulator associated with FAK isoform X1"
                     /calculated_mol_wt=93063
     Region          21..227
                     /region_name="BAR_RhoGAP_OPHN1-like"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain of
                     Oligophrenin1-like Rho GTPase Activating Proteins;
                     cd07602"
                     /db_xref="CDD:153286"
     Site            order(30,33..34,38,40..41,44..45,47..48,51..52,54..55,
                     58..59,61..62,65,92,95..96,188,191..192,194,199,202..203,
                     205..206,210..211,214..215,217..218,220..221,223..224)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153286"
     Region          272..376
                     /region_name="BAR-PH_GRAF_family"
                     /note="GTPase Regulator Associated with Focal adhesion and
                     related proteins Pleckstrin homology (PH) domain; cd01249"
                     /db_xref="CDD:269953"
     Region          369..572
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(421,463,467,536,539..540,563)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238049"
     Site            421
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238049"
     Region          764..816
                     /region_name="SH3_GRAF-like"
                     /note="Src Homology 3 domain of GTPase Regulator
                     Associated with Focal adhesion kinase and similar
                     proteins; cd11882"
                     /db_xref="CDD:212815"
     Site            order(769,771,774,778,797..798,811,813..814)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212815"
     CDS             1..819
                     /gene="Graf"
                     /coded_by="XM_034339573.2:151..2610"
                     /db_xref="GeneID:117611586"
ORIGIN      
        1 mgvglkplef tdcltdspyf renlhyhere lentnqqikr likevkglll aaknlskaqk
       61 afsktlqnfs fecigetqte deshisqslk kfseliasie derdrmlkra ddqiilplen
      121 frkehiggvk egkkkfekqt ikfcqsqery lnlstkkqdn vlqeadatle maerhfcqas
      181 leyvfllqev qerkkfefve tllgfmfgwl tfyhqgheva kdfkpymtel qlkiqktrdn
      241 flatrdktes lmnkmkdmkk savesgvnkl ytregylflm ekkafgttwt kqyctyqkdk
      301 keftmipynq ltgkfsskev ltlascirrm sdtiekrfcf dittvdkpnv vytfqalsee
      361 drklwldamd gkesvsikny aavgastkse envldetgfm fvskciaale ergleeqgly
      421 rvvgvaskvn kllamgldrr kleklnledr fewesktits alktylrtls eplmtfryyn
      481 sfisaakqev kearisdihn lvyrlpkanf nmlvllirhl csvakksdkn lmtvgnlavc
      541 fgpsllrpee etvasimdik fynivveili enceriiagp pqdtirstqn tapprsqrvn
      601 tedgsassgn gtpflkypvh vmssphghhi trpyydgpsa vvtktpsidd rkngdyeeve
      661 htshrippyl dsrsgrvkln nanvmyhsad kvltsgntss ssesiapdph sfssdlpvkq
      721 krgsmmpvhy psgytqrnnp pvsvintspg sgrsspgqvp giwrvrtlya cvaesdgels
      781 fepnqiitnv kaslepgwle gtlngktglv pknyveqlp
//
LOCUS       XP_034195465             795 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase regulator associated with FAK isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195465
VERSION     XP_034195465.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339574.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..795
                     /product="GTPase regulator associated with FAK isoform X2"
                     /calculated_mol_wt=90178
     Region          21..227
                     /region_name="BAR_RhoGAP_OPHN1-like"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain of
                     Oligophrenin1-like Rho GTPase Activating Proteins;
                     cd07602"
                     /db_xref="CDD:153286"
     Site            order(30,33..34,38,40..41,44..45,47..48,51..52,54..55,
                     58..59,61..62,65,92,95..96,188,191..192,194,199,202..203,
                     205..206,210..211,214..215,217..218,220..221,223..224)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153286"
     Region          248..352
                     /region_name="BAR-PH_GRAF_family"
                     /note="GTPase Regulator Associated with Focal adhesion and
                     related proteins Pleckstrin homology (PH) domain; cd01249"
                     /db_xref="CDD:269953"
     Region          345..548
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(397,439,443,512,515..516,539)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238049"
     Site            397
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238049"
     Region          740..792
                     /region_name="SH3_GRAF-like"
                     /note="Src Homology 3 domain of GTPase Regulator
                     Associated with Focal adhesion kinase and similar
                     proteins; cd11882"
                     /db_xref="CDD:212815"
     Site            order(745,747,750,754,773..774,787,789..790)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212815"
     CDS             1..795
                     /gene="Graf"
                     /coded_by="XM_034339574.2:268..2655"
                     /db_xref="GeneID:117611586"
ORIGIN      
        1 mgvglkplef tdcltdspyf renlhyhere lentnqqikr likevkglll aaknlskaqk
       61 afsktlqnfs fecigetqte deshisqslk kfseliasie derdrmlkra ddqiilplen
      121 frkehiggvk egkkkfekqt ikfcqsqery lnlstkkqdn vlqeadatle maerhfcqas
      181 leyvfllqev qerkkfefve tllgfmfgwl tfyhqgheva kdfkpymtel qlkiqksave
      241 sgvnklytre gylflmekka fgttwtkqyc tyqkdkkeft mipynqltgk fsskevltla
      301 scirrmsdti ekrfcfditt vdkpnvvytf qalseedrkl wldamdgkes vsiknyaavg
      361 astkseenvl detgfmfvsk ciaaleergl eeqglyrvvg vaskvnklla mgldrrklek
      421 lnledrfewe sktitsalkt ylrtlseplm tfryynsfis aakqevkear isdihnlvyr
      481 lpkanfnmlv llirhlcsva kksdknlmtv gnlavcfgps llrpeeetva simdikfyni
      541 vveilience riiagppqdt irstqntapp rsqrvntedg sassgngtpf lkypvhvmss
      601 phghhitrpy ydgpsavvtk tpsiddrkng dyeevehtsh rippyldsrs grvklnnanv
      661 myhsadkvlt sgntsssses iapdphsfss dlpvkqkrgs mmpvhypsgy tqrnnppvsv
      721 intspgsgrs spgqvpgiwr vrtlyacvae sdgelsfepn qiitnvkasl epgwlegtln
      781 gktglvpkny veqlp
//
LOCUS       XP_034195467             714 aa            linear   INV 03-SEP-2025
DEFINITION  GTPase regulator associated with FAK isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195467
VERSION     XP_034195467.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339576.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..714
                     /product="GTPase regulator associated with FAK isoform X3"
                     /calculated_mol_wt=80932
     Region          <1..122
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Region          167..271
                     /region_name="BAR-PH_GRAF_family"
                     /note="GTPase Regulator Associated with Focal adhesion and
                     related proteins Pleckstrin homology (PH) domain; cd01249"
                     /db_xref="CDD:269953"
     Region          264..467
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(316,358,362,431,434..435,458)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238049"
     Site            316
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238049"
     Region          659..711
                     /region_name="SH3_GRAF-like"
                     /note="Src Homology 3 domain of GTPase Regulator
                     Associated with Focal adhesion kinase and similar
                     proteins; cd11882"
                     /db_xref="CDD:212815"
     Site            order(664,666,669,673,692..693,706,708..709)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212815"
     CDS             1..714
                     /gene="Graf"
                     /coded_by="XM_034339576.2:134..2278"
                     /db_xref="GeneID:117611586"
ORIGIN      
        1 mlkraddqii lplenfrkeh iggvkegkkk fekqtikfcq sqerylnlst kkqdnvlqea
       61 datlemaerh fcqasleyvf llqevqerkk fefvetllgf mfgwltfyhq ghevakdfkp
      121 ymtelqlkiq ktrdnflatr dkteslmnkm kdmkksaves gvnklytreg ylflmekkaf
      181 gttwtkqyct yqkdkkeftm ipynqltgkf sskevltlas cirrmsdtie krfcfdittv
      241 dkpnvvytfq alseedrklw ldamdgkesv siknyaavga stkseenvld etgfmfvskc
      301 iaaleergle eqglyrvvgv askvnkllam gldrrklekl nledrfewes ktitsalkty
      361 lrtlseplmt fryynsfisa akqevkeari sdihnlvyrl pkanfnmlvl lirhlcsvak
      421 ksdknlmtvg nlavcfgpsl lrpeeetvas imdikfyniv veiliencer iiagppqdti
      481 rstqntappr sqrvntedgs assgngtpfl kypvhvmssp hghhitrpyy dgpsavvtkt
      541 psiddrkngd yeevehtshr ippyldsrsg rvklnnanvm yhsadkvlts gntssssesi
      601 apdphsfssd lpvkqkrgsm mpvhypsgyt qrnnppvsvi ntspgsgrss pgqvpgiwrv
      661 rtlyacvaes dgelsfepnq iitnvkasle pgwlegtlng ktglvpknyv eqlp
//
LOCUS       XP_034195028             619 aa            linear   INV 03-SEP-2025
DEFINITION  brummer isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195028
VERSION     XP_034195028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..619
                     /product="brummer isoform X1"
                     /calculated_mol_wt=69920
     Region          1..245
                     /region_name="Pat_iPLA2"
                     /note="Calcium-independent phospholipase A2; Classified as
                     Group IVA-1 PLA2; cd07218"
                     /db_xref="CDD:132857"
     Site            order(8..9,11,38,157)
                     /site_type="active"
                     /db_xref="CDD:132857"
     Site            36..40
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132857"
     CDS             1..619
                     /gene="bmm"
                     /coded_by="XM_034339137.2:194..2053"
                     /db_xref="GeneID:117611199"
ORIGIN      
        1 mnlsfagcgf lgiyhvgvav cfkkyaphil ldkisgasag aiaaccllcd lplgeitsnv
       61 lrvarearqr tlgpfspsfn vqeilleslq kflpndahir vsgklhvslt rvydgknviv
      121 sqfssredll qallasafip ifsgllpprf hgirymdggf sdnlptlden titvspfcge
      181 sdicprdvss qlfhvnfant sielsrqniy rfarilfppn peilsnmckq gfddalrflh
      241 rnnllnctrc lavqstfvvs etlddnmdyd pecleckmhr qealvsnlpe tvmtifqdai
      301 dsankglinw lfkhksvkll sllslpytlp vdvvyatltn fignkietrs lefkvigril
      361 hthpqmisct khmtssrfil nvpklrrnll elgtffldql siifpkihvy yqqmpqtikc
      421 qlaiteygsn iydmeatkkt dnvyknkknl nltlqynelq pwkdhnnksn cvinmsdlst
      481 vddtfenilq vtseqeavma yyymdennkv qvteifdvtd teshavlsvd evenntklqf
      541 dewdeptwls qhtfndvvte slytdgnqqs ienlseisle ddilgglnts sdpeseweis
      601 kdlqtakals tpnteiemd
//
LOCUS       XP_034195034             582 aa            linear   INV 03-SEP-2025
DEFINITION  brummer isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195034
VERSION     XP_034195034.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..582
                     /product="brummer isoform X2"
                     /calculated_mol_wt=65654
     Region          1..245
                     /region_name="Pat_iPLA2"
                     /note="Calcium-independent phospholipase A2; Classified as
                     Group IVA-1 PLA2; cd07218"
                     /db_xref="CDD:132857"
     Site            order(8..9,11,38,157)
                     /site_type="active"
                     /db_xref="CDD:132857"
     Site            36..40
                     /site_type="other"
                     /note="nucleophile elbow"
                     /db_xref="CDD:132857"
     CDS             1..582
                     /gene="bmm"
                     /coded_by="XM_034339143.2:190..1938"
                     /db_xref="GeneID:117611199"
ORIGIN      
        1 mnlsfagcgf lgiyhvgvav cfkkyaphil ldkisgasag aiaaccllcd lplgeitsnv
       61 lrvarearqr tlgpfspsfn vqeilleslq kflpndahir vsgklhvslt rvydgknviv
      121 sqfssredll qallasafip ifsgllpprf hgirymdggf sdnlptlden titvspfcge
      181 sdicprdvss qlfhvnfant sielsrqniy rfarilfppn peilsnmckq gfddalrflh
      241 rnnllnctrc lavqstfvvs etlddnmdyd pecleckmhr qealvsnlpe tvmtifqdai
      301 dsankglinw lfkhksvkll sllslpytlp vdvvyatltk filnvpklrr nllelgtffl
      361 dqlsiifpki hvyyqqmpqt ikcqlaitey gsniydmeat kktdnvyknk knlnltlqyn
      421 elqpwkdhnn ksncvinmsd lstvddtfen ilqvtseqea vmayyymden nkvqvteifd
      481 vtdteshavl svdevenntk lqfdewdept wlsqhtfndv vteslytdgn qqsienlsei
      541 sleddilggl ntssdpesew eiskdlqtak alstpnteie md
//
LOCUS       XP_034195036             571 aa            linear   INV 03-SEP-2025
DEFINITION  brummer isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195036
VERSION     XP_034195036.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339145.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..571
                     /product="brummer isoform X3"
                     /calculated_mol_wt=65223
     Region          <7..197
                     /region_name="Patatin_and_cPLA2"
                     /note="Patatins and Phospholipases; cl11396"
                     /db_xref="CDD:416256"
     CDS             1..571
                     /gene="bmm"
                     /coded_by="XM_034339145.2:201..1916"
                     /db_xref="GeneID:117611199"
ORIGIN      
        1 mfffareits nvlrvarear qrtlgpfsps fnvqeilles lqkflpndah irvsgklhvs
       61 ltrvydgknv ivsqfssred llqallasaf ipifsgllpp rfhgirymdg gfsdnlptld
      121 entitvspfc gesdicprdv ssqlfhvnfa ntsielsrqn iyrfarilfp pnpeilsnmc
      181 kqgfddalrf lhrnnllnct rclavqstfv vsetlddnmd ydpecleckm hrqealvsnl
      241 petvmtifqd aidsankgli nwlfkhksvk llsllslpyt lpvdvvyatl tnfignkiet
      301 rslefkvigr ilhthpqmis ctkhmtssrf ilnvpklrrn llelgtffld qlsiifpkih
      361 vyyqqmpqti kcqlaiteyg sniydmeatk ktdnvyknkk nlnltlqyne lqpwkdhnnk
      421 sncvinmsdl stvddtfeni lqvtseqeav mayyymdenn kvqvteifdv tdteshavls
      481 vdevenntkl qfdewdeptw lsqhtfndvv teslytdgnq qsienlseis leddilggln
      541 tssdpesewe iskdlqtaka lstpnteiem d
//
LOCUS       XP_034195040             572 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and LEM domain-containing protein 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195040
VERSION     XP_034195040.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339149.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195040.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="ankyrin repeat and LEM domain-containing protein
                     2"
                     /calculated_mol_wt=65524
     Region          163..191
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          164..246
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Site            order(165..166,169..171,173..174,178,181,191,193,195,
                     197..199,201..202,206,209,216,218,220,225..226,229..231,
                     233..234,238,241)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          <511..548
                     /region_name="GST_C_family"
                     /note="C-terminal, alpha helical domain of the Glutathione
                     S-transferase family; cl02776"
                     /db_xref="CDD:470672"
     CDS             1..572
                     /gene="Ankle2"
                     /coded_by="XM_034339149.2:173..1891"
                     /db_xref="GeneID:117611210"
ORIGIN      
        1 mgeneqtsqt llvtygnsts lneplyhavy ipeenldnlp enntkennrv yqdkaealkv
       61 iqkfktgrlk sfkkrseaee yaktgfektn cvnnttlsti vpvveekssd fktprsqdlv
      121 cfrnlikdgd lyavkttiwg npkyligsgd tpailqegcr ynalhiavra drpdmcelil
      181 stvgnaefik lfygdecksy vdraqimldl ylntpdkgln etplhfavkf glkncvrvlv
      241 sypcciktlp nkykqlpidi ictrachddt elkrqirlll edqyyvpvlr sddnslqpvi
      301 gepfsptnpl slntdpispr levrafagpm tksravefrk kwktpprlri tsvrnvndae
      361 ynttdspinn ltlrlqdtek glervgrdla eeyevswkey wpflndfadf rttegltkle
      421 kylehkfqll yysqnsnngv tdsnlntete vklvqeidhl cnelqscsll nivnennvde
      481 lefftppssp eltedssdde mqtaeegpgi flegyipmkv dyavynavss sinpvtypni
      541 yrwqhdmqlv mkhdlhrtnn lrivkkklim tf
//
LOCUS       XP_034195053             572 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and LEM domain-containing protein 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195053
VERSION     XP_034195053.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339162.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195053.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="ankyrin repeat and LEM domain-containing protein
                     2"
                     /calculated_mol_wt=65524
     Region          163..191
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          164..246
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Site            order(165..166,169..171,173..174,178,181,191,193,195,
                     197..199,201..202,206,209,216,218,220,225..226,229..231,
                     233..234,238,241)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          <511..548
                     /region_name="GST_C_family"
                     /note="C-terminal, alpha helical domain of the Glutathione
                     S-transferase family; cl02776"
                     /db_xref="CDD:470672"
     CDS             1..572
                     /gene="Ankle2"
                     /coded_by="XM_034339162.2:159..1877"
                     /db_xref="GeneID:117611210"
ORIGIN      
        1 mgeneqtsqt llvtygnsts lneplyhavy ipeenldnlp enntkennrv yqdkaealkv
       61 iqkfktgrlk sfkkrseaee yaktgfektn cvnnttlsti vpvveekssd fktprsqdlv
      121 cfrnlikdgd lyavkttiwg npkyligsgd tpailqegcr ynalhiavra drpdmcelil
      181 stvgnaefik lfygdecksy vdraqimldl ylntpdkgln etplhfavkf glkncvrvlv
      241 sypcciktlp nkykqlpidi ictrachddt elkrqirlll edqyyvpvlr sddnslqpvi
      301 gepfsptnpl slntdpispr levrafagpm tksravefrk kwktpprlri tsvrnvndae
      361 ynttdspinn ltlrlqdtek glervgrdla eeyevswkey wpflndfadf rttegltkle
      421 kylehkfqll yysqnsnngv tdsnlntete vklvqeidhl cnelqscsll nivnennvde
      481 lefftppssp eltedssdde mqtaeegpgi flegyipmkv dyavynavss sinpvtypni
      541 yrwqhdmqlv mkhdlhrtnn lrivkkklim tf
//
LOCUS       XP_034195048             572 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and LEM domain-containing protein 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195048
VERSION     XP_034195048.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339157.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195048.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..572
                     /product="ankyrin repeat and LEM domain-containing protein
                     2"
                     /calculated_mol_wt=65524
     Region          163..191
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          164..246
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Site            order(165..166,169..171,173..174,178,181,191,193,195,
                     197..199,201..202,206,209,216,218,220,225..226,229..231,
                     233..234,238,241)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          <511..548
                     /region_name="GST_C_family"
                     /note="C-terminal, alpha helical domain of the Glutathione
                     S-transferase family; cl02776"
                     /db_xref="CDD:470672"
     CDS             1..572
                     /gene="Ankle2"
                     /coded_by="XM_034339157.2:158..1876"
                     /db_xref="GeneID:117611210"
ORIGIN      
        1 mgeneqtsqt llvtygnsts lneplyhavy ipeenldnlp enntkennrv yqdkaealkv
       61 iqkfktgrlk sfkkrseaee yaktgfektn cvnnttlsti vpvveekssd fktprsqdlv
      121 cfrnlikdgd lyavkttiwg npkyligsgd tpailqegcr ynalhiavra drpdmcelil
      181 stvgnaefik lfygdecksy vdraqimldl ylntpdkgln etplhfavkf glkncvrvlv
      241 sypcciktlp nkykqlpidi ictrachddt elkrqirlll edqyyvpvlr sddnslqpvi
      301 gepfsptnpl slntdpispr levrafagpm tksravefrk kwktpprlri tsvrnvndae
      361 ynttdspinn ltlrlqdtek glervgrdla eeyevswkey wpflndfadf rttegltkle
      421 kylehkfqll yysqnsnngv tdsnlntete vklvqeidhl cnelqscsll nivnennvde
      481 lefftppssp eltedssdde mqtaeegpgi flegyipmkv dyavynavss sinpvtypni
      541 yrwqhdmqlv mkhdlhrtnn lrivkkklim tf
//
LOCUS       XP_034195058             566 aa            linear   INV 03-SEP-2025
DEFINITION  FMR1-interacting protein NUFIP1 [Osmia lignaria lignaria].
ACCESSION   XP_034195058
VERSION     XP_034195058.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339167.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..566
                     /product="FMR1-interacting protein NUFIP1"
                     /calculated_mol_wt=64867
     Region          234..256
                     /region_name="zf-C2H2_4"
                     /note="C2H2-type zinc finger; pfam13894"
                     /db_xref="CDD:464025"
     Region          <294..330
                     /region_name="NUFIP1"
                     /note="Nuclear fragile X mental retardation-interacting
                     protein 1 (NUFIP1); pfam10453"
                     /db_xref="CDD:463096"
     CDS             1..566
                     /gene="LOC117611228"
                     /coded_by="XM_034339167.2:333..2033"
                     /db_xref="GeneID:117611228"
ORIGIN      
        1 msplnrgpll gprvppagir ppppprfggr lpplafppgm apppinpvfg pirqrlpppp
       61 rpggvnrpsg pmplfgprvr amapmalpmg lrgagprgpl mrpwhrralp pqilshmrpr
      121 fsigngnskg kplnnvkkvs kldelelkkp wmtdeirsei qkknklyaka kknkdakewe
      181 efkdlrnkvt rmirdakndy lakhpeqahl ypsdgepydq rdindtssed desfycevcd
      241 kdfssedals khkqehkfcg idgctfsayp llvekhiamq hstglyhkik nistpediek
      301 wimerkkryp tkvnielrna eklektqrge iikqkqditk qhkrkkllrs rnrkrtvqkq
      361 pteisntiqq ketyrglpef cgtsnlrdtn lqiesktdet ienvpeenin yisdeeeipt
      421 tssesvtttl nipnlvanye ssseesngpl evpikrekld nltveendik kneneiihnt
      481 qssvniqerp apqsseklms kdfkskkkqn iesnrdlknk kqkceinkkq pytsqllqkl
      541 lsrsiqhern licqcvkyiv dnnffd
//
LOCUS       XP_034195527             394 aa            linear   INV 03-SEP-2025
DEFINITION  neutral sphingomyelinase [Osmia lignaria lignaria].
ACCESSION   XP_034195527
VERSION     XP_034195527.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339636.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195527.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..394
                     /product="neutral sphingomyelinase"
                     /calculated_mol_wt=44362
     Region          9..274
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(13,47,134,176,178,232,266..267)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197313"
     Site            order(47,266)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:197313"
     Site            order(134,178,267)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197313"
     CDS             1..394
                     /gene="nSMase"
                     /coded_by="XM_034339636.2:149..1333"
                     /db_xref="GeneID:117611622"
ORIGIN      
        1 mvnevsinvl tlncwgipyv akdrkarmaa iaekfateny dviclqevws sddfkmmkak
       61 tqerlpyshy fhsgvagsgi cilsrypied iifhkwplng yvhkihhgdw fggkgvglck
      121 lkiynlnihf yiahlhaeys rsndeykahr vlqafdtaqf irltsgsads vilggdlnae
      181 sqdlaykimc giagltdacs nssshlgtne cannsytssk larvfpegkr idhilfsgsk
      241 nikievtdfq hpfpkriphk dfsysdheai masfkftsgs ksqctnidik dslkeaikic
      301 erslknvqrq rywylllgfi livpliwsmg ldcsiasyga viginigrlf ltavlcytlf
      361 mssiwniiek nalkaaclgm eiyltnlnnn lykq
//
LOCUS       XP_034195081             370 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611252 [Osmia lignaria lignaria].
ACCESSION   XP_034195081
VERSION     XP_034195081.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339190.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..370
                     /product="uncharacterized protein LOC117611252"
                     /calculated_mol_wt=40266
     Region          19..361
                     /region_name="adenosine_kinase"
                     /note="Adenosine kinase (AK) catalyzes the phosphorylation
                     of ribofuranosyl-containing nucleoside analogues at the
                     5'-hydroxyl using ATP or GTP as the phosphate donor.The
                     physiological function of AK is associated with the
                     regulation of extracellular adenosine...; cd01168"
                     /db_xref="CDD:238573"
     Site            order(24,26,28,49,75..77,80,137,148,150,152,181,212,
                     318..319,322)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238573"
     Site            order(286,289..290,293,305,307,314,317,320..321,324,349,
                     353)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238573"
     CDS             1..370
                     /gene="LOC117611252"
                     /coded_by="XM_034339190.2:218..1330"
                     /db_xref="GeneID:117611252"
ORIGIN      
        1 mfekiplrea iakldvpavv algnplldaf vflknkdllk khnltidget elteekmqel
       61 madlqlesep tisaggsaqn tmrilewlcd etfkkrygiy sgglgndskg ttlknlvrsa
      121 gvdtryaihs tlptgqcial inessrslva nigaagvytt ddfkrcnlsl dkikiiyieg
      181 ffithsfsva kelvtqaqqr niivafnlsg vyifndhhva icemvghani vfgnsremea
      241 laqalnityd dvtdipflln slkriavnvy svytnwwsrg gvfvmtqggs apaiavygkg
      301 ysvqvqpfkp ktpvvdttga gdslvagfla gvlagwepkd cleygckvas fmvtrlgvil
      361 ptnvppdllq
//
LOCUS       XP_034195093             269 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin 13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195093
VERSION     XP_034195093.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339202.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195093.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="syntaxin 13 isoform X1"
                     /calculated_mol_wt=30812
     Region          49..134
                     /region_name="Syntaxin_2"
                     /note="Syntaxin-like protein; pfam14523"
                     /db_xref="CDD:464199"
     Region          176..234
                     /region_name="SNARE_syntaxin7_like"
                     /note="SNARE motif of syntaxin 7, 12 and related
                     sequences; cd15847"
                     /db_xref="CDD:277200"
     Site            order(177,179..180,182..187,189..191,193..194,196..198,
                     200..201,203..205,207..208,210..215,217..222,224..229,
                     231..233)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277200"
     Site            207
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277200"
     CDS             1..269
                     /gene="Syx13"
                     /coded_by="XM_034339202.2:206..1015"
                     /db_xref="GeneID:117611260"
ORIGIN      
        1 mmahssqnyg stdqrsdvpd vgfsptelys lsenittniy tinttwkile ranknigtnk
       61 dnqdlrdkvh vtqlstnqvi tqtskdiarl tvlmrrgdkq qklqiekltt dfkdavqrys
      121 dmqksvaakm kryilpatsi enpmdpddee qqrllqvqed eqqakqrsle fqqgllfere
      181 drvkriegdi ldvnqimrel salvyqqndt intidnnien vhgnvelgaq elikgsnyls
      241 kyrrkvfvll llaiivvvil iiilvtkls
//
LOCUS       XP_076546554             268 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin 13 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546554
VERSION     XP_076546554.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690439.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="syntaxin 13 isoform X2"
                     /calculated_mol_wt=30681
     Region          48..133
                     /region_name="Syntaxin_2"
                     /note="Syntaxin-like protein; pfam14523"
                     /db_xref="CDD:464199"
     Region          175..233
                     /region_name="SNARE_syntaxin7_like"
                     /note="SNARE motif of syntaxin 7, 12 and related
                     sequences; cd15847"
                     /db_xref="CDD:277200"
     Site            order(176,178..179,181..186,188..190,192..193,195..197,
                     199..200,202..204,206..207,209..214,216..221,223..228,
                     230..232)
                     /site_type="other"
                     /note="heterotetramer interface [polypeptide binding]"
                     /db_xref="CDD:277200"
     Site            206
                     /site_type="other"
                     /note="zero layer"
                     /db_xref="CDD:277200"
     CDS             1..268
                     /gene="Syx13"
                     /coded_by="XM_076690439.1:160..966"
                     /db_xref="GeneID:117611260"
ORIGIN      
        1 mahssqnygs tdqrsdvpdv gfsptelysl senittniyt inttwkiler anknigtnkd
       61 nqdlrdkvhv tqlstnqvit qtskdiarlt vlmrrgdkqq klqieklttd fkdavqrysd
      121 mqksvaakmk ryilpatsie npmdpddeeq qrllqvqede qqakqrslef qqgllfered
      181 rvkriegdil dvnqimrels alvyqqndti ntidnnienv hgnvelgaqe likgsnylsk
      241 yrrkvfvlll laiivvvili iilvtkls
//
LOCUS       XP_034195064             462 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin A [Osmia lignaria lignaria].
ACCESSION   XP_034195064
VERSION     XP_034195064.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339173.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="cyclin A"
                     /calculated_mol_wt=53880
     Region          197..324
                     /region_name="CYCLIN_CCNA_rpt1"
                     /note="first cyclin box found in cyclin-A (CCNA) family;
                     cd20504"
                     /db_xref="CDD:410208"
     Site            order(197..199,201..202,205,283,286..290,294..295,
                     308..309,312,315..316,319..320,323..324)
                     /site_type="other"
                     /note="CDK interface [polypeptide binding]"
                     /db_xref="CDD:410208"
     Region          329..438
                     /region_name="CYCLIN_CCNA_rpt2"
                     /note="second cyclin box found in cyclin-A (CCNA) family;
                     cd20505"
                     /db_xref="CDD:410209"
     Site            order(332..333,336)
                     /site_type="other"
                     /note="CDK interface [polypeptide binding]"
                     /db_xref="CDD:410209"
     CDS             1..462
                     /gene="CycA"
                     /coded_by="XM_034339173.2:10..1398"
                     /db_xref="GeneID:117611236"
ORIGIN      
        1 matirvhedq enrivdvrrg kenitvplqn qtlqapkrav lgvlhnnckr nskpeiykde
       61 kfprtkavip tqfepfkiye ekkeevifki yedkleeeas vvlrdtkdkk eikdiqdvkf
      121 erekprlevn pinssnlltf cnatqdidqk keneiifsqd slmsleksvt yssskkdlqk
      181 rresikelra nfydvdeyra diynylriae tqhrpkpgym kkqpditysm rsilvdwlve
      241 vaeeyrlqte tlylavsyid rflsymsvvr aklqlvgtaa mfiaakyeei yppdvgefvy
      301 itddtytkkh vlrmehlilr vlsfdltvpt pltflmeyci snnlsekikf lamylcelsm
      361 legdpylqfl pshlaasaia larytlldem wpheielstg yslkdlkecv iclnttfcna
      421 lniqqqaiqe kyksskyghv alllprridn vtltsedeee na
//
LOCUS       XP_076546555             249 aa            linear   INV 03-SEP-2025
DEFINITION  putative protein FAM10A4 [Osmia lignaria lignaria].
ACCESSION   XP_076546555
VERSION     XP_076546555.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690440.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..249
                     /product="putative protein FAM10A4"
                     /calculated_mol_wt=27677
     Region          7..47
                     /region_name="Hip_N"
                     /note="N-terminal dimerization domain of the
                     Hsp70-interacting protein (Hip) and similar proteins;
                     cd14438"
                     /db_xref="CDD:271228"
     Site            order(9..10,12..14,16..17,20..21,27,31,34..35,37..39,
                     41..47)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:271228"
     Region          <49..141
                     /region_name="LGT"
                     /note="Prolipoprotein diacylglyceryl transferase; cl00478"
                     /db_xref="CDD:469786"
     Site            order(133..134,136,160,163..164,167..168,170..171,194,
                     197..198,201..202,205)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          <139..232
                     /region_name="NlpI"
                     /note="Lipoprotein NlpI, contains TPR repeats [Cell
                     wall/membrane/envelope biogenesis]; COG4785"
                     /db_xref="CDD:443815"
     Region          158..188
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          193..221
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..249
                     /gene="LOC117611245"
                     /coded_by="XM_076690440.1:191..940"
                     /db_xref="GeneID:117611245"
ORIGIN      
        1 msipfkpehl aqlkifvdlc mvdptilnrp elsfiksfve hfggkvpkde psckkpesan
       61 vskepeaeae lgpesepeta aepqaeiees dleldmsdvi epdtdtpqkm gnptlqptke
      121 eiaesqgkra eaisafvekd yegaiqlyte ailinpqasl lyakrghmyl llnkpnacir
      181 dcnravelnp dsaaahkfrg rayhllgkfe eaandlrlac kfdyddeade wlraatpnvs
      241 tfisvcyfv
//
LOCUS       XP_034195106             210 aa            linear   INV 03-SEP-2025
DEFINITION  clathrin light chain isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195106
VERSION     XP_034195106.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339215.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..210
                     /product="clathrin light chain isoform X1"
                     /calculated_mol_wt=23398
     Region          2..207
                     /region_name="Clathrin_lg_ch"
                     /note="Clathrin light chain; pfam01086"
                     /db_xref="CDD:460056"
     CDS             1..210
                     /gene="Clc"
                     /coded_by="XM_034339215.2:156..788"
                     /db_xref="GeneID:117611264"
ORIGIN      
        1 mdafgdnfvi epevdpvaef vareqdqlag ledeippvsi tapttisntd vgpggdaegs
       61 feiidavgqp tdpqappvie taskpppvke epekirkwre eqkarleekd aeeekkkeew
      121 reaarkelee wykhhaeais ktkttnresa knaekqfvae adevepgtew eriaklcdfn
      181 pkssraskdv srmrsiilql kqtppapvsl
//
LOCUS       XP_034195119             206 aa            linear   INV 03-SEP-2025
DEFINITION  clathrin light chain isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195119
VERSION     XP_034195119.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339228.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..206
                     /product="clathrin light chain isoform X2"
                     /calculated_mol_wt=22983
     Region          2..203
                     /region_name="Clathrin_lg_ch"
                     /note="Clathrin light chain; pfam01086"
                     /db_xref="CDD:460056"
     CDS             1..206
                     /gene="Clc"
                     /coded_by="XM_034339228.2:154..774"
                     /db_xref="GeneID:117611264"
ORIGIN      
        1 mdafgdnfvi epevdpvaef vareqdqlag ledeippvsi tapttisntd vgpggdaegs
       61 feiidavgqp tdpqappvie taskpppvke epekirkwre eqkarleekd aeeekkkeew
      121 reaarkelee wykhhaeais ktkttnrnae kqfvaeadev epgteweria klcdfnpkss
      181 raskdvsrmr siilqlkqtp papvsl
//
LOCUS       XP_034195127             197 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger matrin-type protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034195127
VERSION     XP_034195127.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339236.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..197
                     /product="zinc finger matrin-type protein 2"
                     /calculated_mol_wt=23026
     Region          75..106
                     /region_name="ZnF_U1"
                     /note="U1-like zinc finger; smart00451"
                     /db_xref="CDD:197732"
     CDS             1..197
                     /gene="LOC117611278"
                     /coded_by="XM_034339236.2:151..744"
                     /db_xref="GeneID:117611278"
ORIGIN      
        1 msmrpddhrr kwnreeyeri alqrlqdeia eeelgipkqp avkrellkqr dykvdleskl
       61 gksvvinknt pssqtggyyc nvcdcvvkds infldhingt khqrnlgmsm kierstleqv
      121 karfamnkkk leekkkdydl eqrvkelkee eekikeyrke krkdkkrkie eisddnggps
      181 demaaimgfs gfgskkk
//
LOCUS       XP_034195136             180 aa            linear   INV 03-SEP-2025
DEFINITION  ADP ribosylation factor-like 1 [Osmia lignaria lignaria].
ACCESSION   XP_034195136
VERSION     XP_034195136.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339245.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..180
                     /product="ADP ribosylation factor-like 1"
                     /calculated_mol_wt=20044
     Region          18..175
                     /region_name="Arl1"
                     /note="ADP ribosylation factor 1 (Arf1); cd04151"
                     /db_xref="CDD:206718"
     Site            23..30
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206718"
     Site            order(25..31,44,47,69,125..126,128,158..160)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206718"
     Site            order(25..26,30,34,46..53,75,80)
                     /site_type="other"
                     /note="putative GAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206718"
     Site            35..50
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206718"
     Site            order(47..51,56,66,70,76,78..80)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206718"
     Site            47
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206718"
     Site            order(49..50,63,79..80,82,114)
                     /site_type="active"
                     /note="effector interaction site [active]"
                     /db_xref="CDD:206718"
     Site            order(50..57,60..65)
                     /site_type="active"
                     /note="interswitch region [active]"
                     /db_xref="CDD:206718"
     Site            order(66..74,76..79)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206718"
     Site            66..69
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206718"
     Site            125..128
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206718"
     Site            158..160
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206718"
     CDS             1..180
                     /gene="Arl1"
                     /coded_by="XM_034339245.2:186..728"
                     /db_xref="GeneID:117611289"
ORIGIN      
        1 mggllsyfrn llgsremril ilgldgagkt tilyrlqvge vvttiptigf nveqvtyknl
       61 kfqvwdlggq tsirpywrcy ysntdaiiyv vdsadkdrig iskdeliyml reeelqgail
      121 vvlankqdma gclsvaevhq algldalknr tfqifktsat kgegldqamd wlsnalqsrk
//
LOCUS       XP_076546556             180 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305724 [Osmia lignaria lignaria].
ACCESSION   XP_076546556
VERSION     XP_076546556.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690441.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..180
                     /product="uncharacterized protein LOC143305724"
                     /calculated_mol_wt=19790
     Region          87..135
                     /region_name="STI1"
                     /note="STI1 domain; cl17974"
                     /db_xref="CDD:450205"
     CDS             1..180
                     /gene="LOC143305724"
                     /coded_by="XM_076690441.1:285..827"
                     /db_xref="GeneID:143305724"
ORIGIN      
        1 mclgqtvlfh gklclnikgf instcyfqaw kivehkrkke rkmqekvere rqdriknaqe
       61 sakafqenfr tfqadptgsa pagmpdfskf lddaeivqsl mepeiaefck eissnpasil
      121 kyqrnpkima finkmaskfg gagipgmpgg mpnfpstdga appppppppp steqsdvgld
//
LOCUS       XP_034195138             125 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611294 [Osmia lignaria lignaria].
ACCESSION   XP_034195138
VERSION     XP_034195138.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339247.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195138.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="uncharacterized protein LOC117611294"
                     /calculated_mol_wt=15197
     CDS             1..125
                     /gene="LOC117611294"
                     /coded_by="XM_034339247.2:1..378"
                     /db_xref="GeneID:117611294"
ORIGIN      
        1 mdlsnlehal qssrrklqrh hhhhhhhhhh lhrnhhhhhh hrrhqkkhrr vvsdpgtrnw
       61 ntddlevkrh rrmfsydtgi tglrlletpv teadkevlsp detktstfrk vpwhpnlhml
      121 kirrt
//
LOCUS       XP_034194492            1487 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 1A isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034194492
VERSION     XP_034194492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1487
                     /product="ankyrin repeat and SAM domain-containing protein
                     1A isoform X1"
                     /calculated_mol_wt=161937
     Region          7..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <26..264
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(52,54,58..59,62..64,66..67,71,74,83,85,87,91..92,
                     95..97,99..100,104,107,119,121,123,127..128,131..133,
                     135..136,140,143,152)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          85..119
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          121..152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          154..225
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          200..>332
                     /region_name="PTZ00322"
                     /note="6-phosphofructo-2-kinase/fructose-2,
                     6-biphosphatase; Provisional"
                     /db_xref="CDD:140343"
     Region          227..257
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          925..990
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          995..1058
                     /region_name="SAM_AIDA1AB-like_repeat2"
                     /note="SAM domain of AIDA1AB-like proteins, repeat 2;
                     cd09500"
                     /db_xref="CDD:188899"
     Site            order(1044..1047,1049..1050)
                     /site_type="other"
                     /note="intramolecular dimer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188899"
     Region          1167..1310
                     /region_name="PTB_Anks"
                     /note="Ankyrin repeat and sterile alpha motif (SAM)
                     domain-containing (Anks) protein family
                     Phosphotyrosine-binding (PTB) domain; cd01274"
                     /db_xref="CDD:269972"
     Site            order(1186,1261,1281)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269972"
     Site            order(1250..1255,1268,1292,1296)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269972"
     CDS             1..1487
                     /gene="LOC117610792"
                     /coded_by="XM_034338601.2:152..4615"
                     /db_xref="GeneID:117610792"
ORIGIN      
        1 mgkdqellea arsgnvtvve kilgqrakrs gplarwvspl rrgpganvqd asgysalhha
       61 alnghkevvk lllqyeastn vvdakgsspl hlaawagnae ivrliltqgp svpkvnlttk
      121 dnetalhcaa qyghtevvaq llqygcdpsi rnsrgesald laaqygrlet vqllvstype
      181 livplrnsss svifphtplh lasrnghrav vevllaagvd vntrtsagta mheaalcgkm
      241 evvralldrg vdlgirdsrq ntvldllgqf pphvtqdita vikrhrsssg vesdadsenl
      301 ppipvqggds lgspyenvrp gddlspaegt sptqwqfyhk prdddrrvsg tsvmsfeder
      361 nggpvngvrr tlqqtedsdp sslfdsvfms msdtlnsint fessdqtpdd kpqntttnri
      421 plaqrdttrg sdsglyqtpp vprgtmegsf vsedlsltlp tgygggresd qlsvsssssv
      481 ggpsprdrrc lpndssaagi ylpmaplsss lhspasnskv sptppkkppr rnlsvspthl
      541 qtqmslsadc tlgpsnyeyl fmarsgarsh idleqlqkrr eqlrhvtrsv dqyvemksrv
      601 pdgeerrdan vepvaitsiy enrpiktlnp rrklrrhafd nyeaesspca dkspcsetdm
      661 faqeqtfvsn afltlkssqe slldekrdtv deqsvpegre atdkrlkrvq mmlptspthy
      721 aqpptpdhpp psamqaeksi herirplsqv ykrrsrdmet etdedllqfp aggsldassg
      781 slssvslsdk smstdnveey fgdvpfagll kgsvtaerpr tlrrlrnvyg paacgmgsgg
      841 eggggerled gevpfrlsdr drerdeksls imspfdeqee wakiseimas fgtglvresv
      901 fvtelekefq trlglssdts sspivstsvg qwlstlglad yetlfinygf ddldfingvl
      961 deadlkdmgi ssdqeravim daagllnkrv dkrsggnsqs vdewlkiihl enyaetfkkh
     1021 lytdmdrvrr vwevelaavl eiqkpahrkr ilasvsgssa raqnrnssgp nledlnkdln
     1081 tlktniqqlk eeireklpet ttegnggtsi agtnstttgt lrhrknrpap qppqrpsshn
     1141 gaisapeqle irapselllg vpstlktqwr hqpkalvtgc vtyvanylgs tvvkelrgte
     1201 stkksiqklk ktcreprvtp ditlaisyrg vrflntvtne piceheirni hcacqdaddl
     1261 thfayitkdh asrthfchvf cvptmdqate viltlgqafe vayqmalrdk lgghtvrsqs
     1321 anqlttlsks pasikmsisp dstpdgnlvd qpaaavtnan ltcpnpklkp rskssipnpi
     1381 vqsvvpslah dmnsnsssgs saianctsss npatspstns vsssnsntst nqtakplvth
     1441 grshsvndik ingnqlklap tpiddigigv kdmkpgsrap ialseel
//
LOCUS       XP_034194502            1487 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 1A isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034194502
VERSION     XP_034194502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1487
                     /product="ankyrin repeat and SAM domain-containing protein
                     1A isoform X1"
                     /calculated_mol_wt=161937
     Region          7..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <26..264
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(52,54,58..59,62..64,66..67,71,74,83,85,87,91..92,
                     95..97,99..100,104,107,119,121,123,127..128,131..133,
                     135..136,140,143,152)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          85..119
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          121..152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          154..225
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          200..>332
                     /region_name="PTZ00322"
                     /note="6-phosphofructo-2-kinase/fructose-2,
                     6-biphosphatase; Provisional"
                     /db_xref="CDD:140343"
     Region          227..257
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          925..990
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          995..1058
                     /region_name="SAM_AIDA1AB-like_repeat2"
                     /note="SAM domain of AIDA1AB-like proteins, repeat 2;
                     cd09500"
                     /db_xref="CDD:188899"
     Site            order(1044..1047,1049..1050)
                     /site_type="other"
                     /note="intramolecular dimer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188899"
     Region          1167..1310
                     /region_name="PTB_Anks"
                     /note="Ankyrin repeat and sterile alpha motif (SAM)
                     domain-containing (Anks) protein family
                     Phosphotyrosine-binding (PTB) domain; cd01274"
                     /db_xref="CDD:269972"
     Site            order(1186,1261,1281)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269972"
     Site            order(1250..1255,1268,1292,1296)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269972"
     CDS             1..1487
                     /gene="LOC117610792"
                     /coded_by="XM_034338611.2:153..4616"
                     /db_xref="GeneID:117610792"
ORIGIN      
        1 mgkdqellea arsgnvtvve kilgqrakrs gplarwvspl rrgpganvqd asgysalhha
       61 alnghkevvk lllqyeastn vvdakgsspl hlaawagnae ivrliltqgp svpkvnlttk
      121 dnetalhcaa qyghtevvaq llqygcdpsi rnsrgesald laaqygrlet vqllvstype
      181 livplrnsss svifphtplh lasrnghrav vevllaagvd vntrtsagta mheaalcgkm
      241 evvralldrg vdlgirdsrq ntvldllgqf pphvtqdita vikrhrsssg vesdadsenl
      301 ppipvqggds lgspyenvrp gddlspaegt sptqwqfyhk prdddrrvsg tsvmsfeder
      361 nggpvngvrr tlqqtedsdp sslfdsvfms msdtlnsint fessdqtpdd kpqntttnri
      421 plaqrdttrg sdsglyqtpp vprgtmegsf vsedlsltlp tgygggresd qlsvsssssv
      481 ggpsprdrrc lpndssaagi ylpmaplsss lhspasnskv sptppkkppr rnlsvspthl
      541 qtqmslsadc tlgpsnyeyl fmarsgarsh idleqlqkrr eqlrhvtrsv dqyvemksrv
      601 pdgeerrdan vepvaitsiy enrpiktlnp rrklrrhafd nyeaesspca dkspcsetdm
      661 faqeqtfvsn afltlkssqe slldekrdtv deqsvpegre atdkrlkrvq mmlptspthy
      721 aqpptpdhpp psamqaeksi herirplsqv ykrrsrdmet etdedllqfp aggsldassg
      781 slssvslsdk smstdnveey fgdvpfagll kgsvtaerpr tlrrlrnvyg paacgmgsgg
      841 eggggerled gevpfrlsdr drerdeksls imspfdeqee wakiseimas fgtglvresv
      901 fvtelekefq trlglssdts sspivstsvg qwlstlglad yetlfinygf ddldfingvl
      961 deadlkdmgi ssdqeravim daagllnkrv dkrsggnsqs vdewlkiihl enyaetfkkh
     1021 lytdmdrvrr vwevelaavl eiqkpahrkr ilasvsgssa raqnrnssgp nledlnkdln
     1081 tlktniqqlk eeireklpet ttegnggtsi agtnstttgt lrhrknrpap qppqrpsshn
     1141 gaisapeqle irapselllg vpstlktqwr hqpkalvtgc vtyvanylgs tvvkelrgte
     1201 stkksiqklk ktcreprvtp ditlaisyrg vrflntvtne piceheirni hcacqdaddl
     1261 thfayitkdh asrthfchvf cvptmdqate viltlgqafe vayqmalrdk lgghtvrsqs
     1321 anqlttlsks pasikmsisp dstpdgnlvd qpaaavtnan ltcpnpklkp rskssipnpi
     1381 vqsvvpslah dmnsnsssgs saianctsss npatspstns vsssnsntst nqtakplvth
     1441 grshsvndik ingnqlklap tpiddigigv kdmkpgsrap ialseel
//
LOCUS       XP_076546557            1483 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 1A isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_076546557
VERSION     XP_076546557.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690442.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1483
                     /product="ankyrin repeat and SAM domain-containing protein
                     1A isoform X2"
                     /calculated_mol_wt=161398
     Region          7..46
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          33..262
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(48,50,54..55,58..60,62..63,67,70,79,81,83,87..88,
                     91..93,95..96,100,103,115,117,119,123..124,127..129,
                     131..132,136,139,148)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          48..79
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          81..115
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          117..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          150..221
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          223..253
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          921..986
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          991..1054
                     /region_name="SAM_AIDA1AB-like_repeat2"
                     /note="SAM domain of AIDA1AB-like proteins, repeat 2;
                     cd09500"
                     /db_xref="CDD:188899"
     Site            order(1040..1043,1045..1046)
                     /site_type="other"
                     /note="intramolecular dimer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188899"
     Region          1163..1306
                     /region_name="PTB_Anks"
                     /note="Ankyrin repeat and sterile alpha motif (SAM)
                     domain-containing (Anks) protein family
                     Phosphotyrosine-binding (PTB) domain; cd01274"
                     /db_xref="CDD:269972"
     Site            order(1182,1257,1277)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269972"
     Site            order(1246..1251,1264,1288,1292)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269972"
     CDS             1..1483
                     /gene="LOC117610792"
                     /coded_by="XM_076690442.1:152..4603"
                     /db_xref="GeneID:117610792"
ORIGIN      
        1 mgkdqellea arsgnvtvve kilgqrakrs gplaslrrgp ganvqdasgy salhhaalng
       61 hkevvklllq yeastnvvda kgssplhlaa wagnaeivrl iltqgpsvpk vnlttkdnet
      121 alhcaaqygh tevvaqllqy gcdpsirnsr gesaldlaaq ygrletvqll vstypelivp
      181 lrnssssvif phtplhlasr nghravvevl laagvdvntr tsagtamhea alcgkmevvr
      241 alldrgvdlg irdsrqntvl dllgqfpphv tqditavikr hrsssgvesd adsenlppip
      301 vqggdslgsp yenvrpgddl spaegtsptq wqfyhkprdd drrvsgtsvm sfedernggp
      361 vngvrrtlqq tedsdpsslf dsvfmsmsdt lnsintfess dqtpddkpqn tttnriplaq
      421 rdttrgsdsg lyqtppvprg tmegsfvsed lsltlptgyg ggresdqlsv sssssvggps
      481 prdrrclpnd ssaagiylpm aplssslhsp asnskvsptp pkkpprrnls vspthlqtqm
      541 slsadctlgp snyeylfmar sgarshidle qlqkrreqlr hvtrsvdqyv emksrvpdge
      601 errdanvepv aitsiyenrp iktlnprrkl rrhafdnyea esspcadksp csetdmfaqe
      661 qtfvsnaflt lkssqeslld ekrdtvdeqs vpegreatdk rlkrvqmmlp tspthyaqpp
      721 tpdhpppsam qaeksiheri rplsqvykrr srdmetetde dllqfpaggs ldassgslss
      781 vslsdksmst dnveeyfgdv pfagllkgsv taerprtlrr lrnvygpaac gmgsggeggg
      841 gerledgevp frlsdrdrer dekslsimsp fdeqeewaki seimasfgtg lvresvfvte
      901 lekefqtrlg lssdtssspi vstsvgqwls tlgladyetl finygfddld fingvldead
      961 lkdmgissdq eravimdaag llnkrvdkrs ggnsqsvdew lkiihlenya etfkkhlytd
     1021 mdrvrrvwev elaavleiqk pahrkrilas vsgssaraqn rnssgpnled lnkdlntlkt
     1081 niqqlkeeir eklpettteg nggtsiagtn stttgtlrhr knrpapqppq rpsshngais
     1141 apeqleirap selllgvpst lktqwrhqpk alvtgcvtyv anylgstvvk elrgtestkk
     1201 siqklkktcr eprvtpditl aisyrgvrfl ntvtnepice heirnihcac qdaddlthfa
     1261 yitkdhasrt hfchvfcvpt mdqatevilt lgqafevayq malrdklggh tvrsqsanql
     1321 ttlskspasi kmsispdstp dgnlvdqpaa avtnanltcp npklkprsks sipnpivqsv
     1381 vpslahdmns nsssgssaia nctsssnpat spstnsvsss nsntstnqta kplvthgrsh
     1441 svndikingn qlklaptpid digigvkdmk pgsrapials eel
//
LOCUS       XP_034194511            1483 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 1A isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034194511
VERSION     XP_034194511.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338620.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1483
                     /product="ankyrin repeat and SAM domain-containing protein
                     1A isoform X2"
                     /calculated_mol_wt=161398
     Region          7..46
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          33..262
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(48,50,54..55,58..60,62..63,67,70,79,81,83,87..88,
                     91..93,95..96,100,103,115,117,119,123..124,127..129,
                     131..132,136,139,148)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          48..79
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          81..115
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          117..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          150..221
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          223..253
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          921..986
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          991..1054
                     /region_name="SAM_AIDA1AB-like_repeat2"
                     /note="SAM domain of AIDA1AB-like proteins, repeat 2;
                     cd09500"
                     /db_xref="CDD:188899"
     Site            order(1040..1043,1045..1046)
                     /site_type="other"
                     /note="intramolecular dimer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188899"
     Region          1163..1306
                     /region_name="PTB_Anks"
                     /note="Ankyrin repeat and sterile alpha motif (SAM)
                     domain-containing (Anks) protein family
                     Phosphotyrosine-binding (PTB) domain; cd01274"
                     /db_xref="CDD:269972"
     Site            order(1182,1257,1277)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269972"
     Site            order(1246..1251,1264,1288,1292)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269972"
     CDS             1..1483
                     /gene="LOC117610792"
                     /coded_by="XM_034338620.2:153..4604"
                     /db_xref="GeneID:117610792"
ORIGIN      
        1 mgkdqellea arsgnvtvve kilgqrakrs gplaslrrgp ganvqdasgy salhhaalng
       61 hkevvklllq yeastnvvda kgssplhlaa wagnaeivrl iltqgpsvpk vnlttkdnet
      121 alhcaaqygh tevvaqllqy gcdpsirnsr gesaldlaaq ygrletvqll vstypelivp
      181 lrnssssvif phtplhlasr nghravvevl laagvdvntr tsagtamhea alcgkmevvr
      241 alldrgvdlg irdsrqntvl dllgqfpphv tqditavikr hrsssgvesd adsenlppip
      301 vqggdslgsp yenvrpgddl spaegtsptq wqfyhkprdd drrvsgtsvm sfedernggp
      361 vngvrrtlqq tedsdpsslf dsvfmsmsdt lnsintfess dqtpddkpqn tttnriplaq
      421 rdttrgsdsg lyqtppvprg tmegsfvsed lsltlptgyg ggresdqlsv sssssvggps
      481 prdrrclpnd ssaagiylpm aplssslhsp asnskvsptp pkkpprrnls vspthlqtqm
      541 slsadctlgp snyeylfmar sgarshidle qlqkrreqlr hvtrsvdqyv emksrvpdge
      601 errdanvepv aitsiyenrp iktlnprrkl rrhafdnyea esspcadksp csetdmfaqe
      661 qtfvsnaflt lkssqeslld ekrdtvdeqs vpegreatdk rlkrvqmmlp tspthyaqpp
      721 tpdhpppsam qaeksiheri rplsqvykrr srdmetetde dllqfpaggs ldassgslss
      781 vslsdksmst dnveeyfgdv pfagllkgsv taerprtlrr lrnvygpaac gmgsggeggg
      841 gerledgevp frlsdrdrer dekslsimsp fdeqeewaki seimasfgtg lvresvfvte
      901 lekefqtrlg lssdtssspi vstsvgqwls tlgladyetl finygfddld fingvldead
      961 lkdmgissdq eravimdaag llnkrvdkrs ggnsqsvdew lkiihlenya etfkkhlytd
     1021 mdrvrrvwev elaavleiqk pahrkrilas vsgssaraqn rnssgpnled lnkdlntlkt
     1081 niqqlkeeir eklpettteg nggtsiagtn stttgtlrhr knrpapqppq rpsshngais
     1141 apeqleirap selllgvpst lktqwrhqpk alvtgcvtyv anylgstvvk elrgtestkk
     1201 siqklkktcr eprvtpditl aisyrgvrfl ntvtnepice heirnihcac qdaddlthfa
     1261 yitkdhasrt hfchvfcvpt mdqatevilt lgqafevayq malrdklggh tvrsqsanql
     1321 ttlskspasi kmsispdstp dgnlvdqpaa avtnanltcp npklkprsks sipnpivqsv
     1381 vpslahdmns nsssgssaia nctsssnpat spstnsvsss nsntstnqta kplvthgrsh
     1441 svndikingn qlklaptpid digigvkdmk pgsrapials eel
//
LOCUS       XP_076546558            1446 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and sterile alpha motif domain-containing protein 1B
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546558
VERSION     XP_076546558.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690443.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1446
                     /product="ankyrin repeat and sterile alpha motif
                     domain-containing protein 1B isoform X3"
                     /calculated_mol_wt=157374
     Region          7..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <26..225
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(52,54,58..59,62..64,66..67,71,74,83,85,87,91..92,
                     95..97,99..100,104,107,119,121,123,127..128,131..133,
                     135..136,140,143,152)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          85..119
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          121..152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          154..184
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          186..216
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          884..949
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          954..1017
                     /region_name="SAM_AIDA1AB-like_repeat2"
                     /note="SAM domain of AIDA1AB-like proteins, repeat 2;
                     cd09500"
                     /db_xref="CDD:188899"
     Site            order(1003..1006,1008..1009)
                     /site_type="other"
                     /note="intramolecular dimer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188899"
     Region          1126..1269
                     /region_name="PTB_Anks"
                     /note="Ankyrin repeat and sterile alpha motif (SAM)
                     domain-containing (Anks) protein family
                     Phosphotyrosine-binding (PTB) domain; cd01274"
                     /db_xref="CDD:269972"
     Site            order(1145,1220,1240)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269972"
     Site            order(1209..1214,1227,1251,1255)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269972"
     CDS             1..1446
                     /gene="LOC117610792"
                     /coded_by="XM_076690443.1:152..4492"
                     /db_xref="GeneID:117610792"
ORIGIN      
        1 mgkdqellea arsgnvtvve kilgqrakrs gplarwvspl rrgpganvqd asgysalhha
       61 alnghkevvk lllqyeastn vvdakgsspl hlaawagnae ivrliltqgp svpkvnlttk
      121 dnetalhcaa qyghtevvaq llqygcdpsi rnsrgesald laaqygravv evllaagvdv
      181 ntrtsagtam heaalcgkme vvralldrgv dlgirdsrqn tvldllgqfp phvtqditav
      241 ikrhrsssgv esdadsenlp pipvqggdsl gspyenvrpg ddlspaegts ptqwqfyhkp
      301 rdddrrvsgt svmsfedern ggpvngvrrt lqqtedsdps slfdsvfmsm sdtlnsintf
      361 essdqtpddk pqntttnrip laqrdttrgs dsglyqtppv prgtmegsfv sedlsltlpt
      421 gygggresdq lsvsssssvg gpsprdrrcl pndssaagiy lpmaplsssl hspasnskvs
      481 ptppkkpprr nlsvspthlq tqmslsadct lgpsnyeylf marsgarshi dleqlqkrre
      541 qlrhvtrsvd qyvemksrvp dgeerrdanv epvaitsiye nrpiktlnpr rklrrhafdn
      601 yeaesspcad kspcsetdmf aqeqtfvsna fltlkssqes lldekrdtvd eqsvpegrea
      661 tdkrlkrvqm mlptspthya qpptpdhppp samqaeksih erirplsqvy krrsrdmete
      721 tdedllqfpa ggsldassgs lssvslsdks mstdnveeyf gdvpfagllk gsvtaerprt
      781 lrrlrnvygp aacgmgsgge ggggerledg evpfrlsdrd rerdekslsi mspfdeqeew
      841 akiseimasf gtglvresvf vtelekefqt rlglssdtss spivstsvgq wlstlglady
      901 etlfinygfd dldfingvld eadlkdmgis sdqeravimd aagllnkrvd krsggnsqsv
      961 dewlkiihle nyaetfkkhl ytdmdrvrrv wevelaavle iqkpahrkri lasvsgssar
     1021 aqnrnssgpn ledlnkdlnt lktniqqlke eireklpett tegnggtsia gtnstttgtl
     1081 rhrknrpapq ppqrpsshng aisapeqlei rapselllgv pstlktqwrh qpkalvtgcv
     1141 tyvanylgst vvkelrgtes tkksiqklkk tcreprvtpd itlaisyrgv rflntvtnep
     1201 iceheirnih cacqdaddlt hfayitkdha srthfchvfc vptmdqatev iltlgqafev
     1261 ayqmalrdkl gghtvrsqsa nqlttlsksp asikmsispd stpdgnlvdq paaavtnanl
     1321 tcpnpklkpr skssipnpiv qsvvpslahd mnsnsssgss aianctsssn patspstnsv
     1381 sssnsntstn qtakplvthg rshsvndiki ngnqlklapt piddigigvk dmkpgsrapi
     1441 alseel
//
LOCUS       XP_034194521            1414 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 1A isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034194521
VERSION     XP_034194521.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338630.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1414
                     /product="ankyrin repeat and SAM domain-containing protein
                     1A isoform X4"
                     /calculated_mol_wt=153757
     Region          7..50
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <26..264
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(52,54,58..59,62..64,66..67,71,74,83,85,87,91..92,
                     95..97,99..100,104,107,119,121,123,127..128,131..133,
                     135..136,140,143,152)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          52..83
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          85..119
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          121..152
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          154..225
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          200..>332
                     /region_name="PTZ00322"
                     /note="6-phosphofructo-2-kinase/fructose-2,
                     6-biphosphatase; Provisional"
                     /db_xref="CDD:140343"
     Region          227..257
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          852..917
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          922..985
                     /region_name="SAM_AIDA1AB-like_repeat2"
                     /note="SAM domain of AIDA1AB-like proteins, repeat 2;
                     cd09500"
                     /db_xref="CDD:188899"
     Site            order(971..974,976..977)
                     /site_type="other"
                     /note="intramolecular dimer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188899"
     Region          1094..1237
                     /region_name="PTB_Anks"
                     /note="Ankyrin repeat and sterile alpha motif (SAM)
                     domain-containing (Anks) protein family
                     Phosphotyrosine-binding (PTB) domain; cd01274"
                     /db_xref="CDD:269972"
     Site            order(1113,1188,1208)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269972"
     Site            order(1177..1182,1195,1219,1223)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269972"
     CDS             1..1414
                     /gene="LOC117610792"
                     /coded_by="XM_034338630.2:152..4396"
                     /db_xref="GeneID:117610792"
ORIGIN      
        1 mgkdqellea arsgnvtvve kilgqrakrs gplarwvspl rrgpganvqd asgysalhha
       61 alnghkevvk lllqyeastn vvdakgsspl hlaawagnae ivrliltqgp svpkvnlttk
      121 dnetalhcaa qyghtevvaq llqygcdpsi rnsrgesald laaqygrlet vqllvstype
      181 livplrnsss svifphtplh lasrnghrav vevllaagvd vntrtsagta mheaalcgkm
      241 evvralldrg vdlgirdsrq ntvldllgqf pphvtqdita vikrhrsssg vesdadsenl
      301 ppipvqggds lgspyenvrp gddlspaegt sptqwqfyhk prdddrrvsg tsvmslgsds
      361 glyqtppvpr gtmegsfvse dlsltlptgy gggresdqls vsssssvggp sprdrrclpn
      421 dssaagiylp maplssslhs pasnskvspt ppkkpprrnl svspthlqtq mslsadctlg
      481 psnyeylfma rsgarshidl eqlqkrreql rhvtrsvdqy vemksrvpdg eerrdanvep
      541 vaitsiyenr piktlnprrk lrrhafdnye aesspcadks pcsetdmfaq eqtfvsnafl
      601 tlkssqesll dekrdtvdeq svpegreatd krlkrvqmml ptspthyaqp ptpdhpppsa
      661 mqaeksiher irplsqvykr rsrdmetetd edllqfpagg sldassgsls svslsdksms
      721 tdnveeyfgd vpfagllkgs vtaerprtlr rlrnvygpaa cgmgsggegg ggerledgev
      781 pfrlsdrdre rdekslsims pfdeqeewak iseimasfgt glvresvfvt elekefqtrl
      841 glssdtsssp ivstsvgqwl stlgladyet lfinygfddl dfingvldea dlkdmgissd
      901 qeravimdaa gllnkrvdkr sggnsqsvde wlkiihleny aetfkkhlyt dmdrvrrvwe
      961 velaavleiq kpahrkrila svsgssaraq nrnssgpnle dlnkdlntlk tniqqlkeei
     1021 reklpettte gnggtsiagt nstttgtlrh rknrpapqpp qrpsshngai sapeqleira
     1081 pselllgvps tlktqwrhqp kalvtgcvty vanylgstvv kelrgtestk ksiqklkktc
     1141 reprvtpdit laisyrgvrf lntvtnepic eheirnihca cqdaddlthf ayitkdhasr
     1201 thfchvfcvp tmdqatevil tlgqafevay qmalrdklgg htvrsqsanq lttlskspas
     1261 ikmsispdst pdgnlvdqpa aavtnanltc pnpklkprsk ssipnpivqs vvpslahdmn
     1321 snsssgssai anctsssnpa tspstnsvss snsntstnqt akplvthgrs hsvndiking
     1381 nqlklaptpi ddigigvkdm kpgsrapial seel
//
LOCUS       XP_034194529            1410 aa            linear   INV 03-SEP-2025
DEFINITION  ankyrin repeat and SAM domain-containing protein 1A isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034194529
VERSION     XP_034194529.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338638.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1410
                     /product="ankyrin repeat and SAM domain-containing protein
                     1A isoform X5"
                     /calculated_mol_wt=153218
     Region          7..46
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          33..262
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(48,50,54..55,58..60,62..63,67,70,79,81,83,87..88,
                     91..93,95..96,100,103,115,117,119,123..124,127..129,
                     131..132,136,139,148)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          48..79
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          81..115
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          117..148
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          150..221
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          223..253
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          848..913
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     Region          918..981
                     /region_name="SAM_AIDA1AB-like_repeat2"
                     /note="SAM domain of AIDA1AB-like proteins, repeat 2;
                     cd09500"
                     /db_xref="CDD:188899"
     Site            order(967..970,972..973)
                     /site_type="other"
                     /note="intramolecular dimer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188899"
     Region          1090..1233
                     /region_name="PTB_Anks"
                     /note="Ankyrin repeat and sterile alpha motif (SAM)
                     domain-containing (Anks) protein family
                     Phosphotyrosine-binding (PTB) domain; cd01274"
                     /db_xref="CDD:269972"
     Site            order(1109,1184,1204)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:269972"
     Site            order(1173..1178,1191,1215,1219)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:269972"
     CDS             1..1410
                     /gene="LOC117610792"
                     /coded_by="XM_034338638.2:152..4384"
                     /db_xref="GeneID:117610792"
ORIGIN      
        1 mgkdqellea arsgnvtvve kilgqrakrs gplaslrrgp ganvqdasgy salhhaalng
       61 hkevvklllq yeastnvvda kgssplhlaa wagnaeivrl iltqgpsvpk vnlttkdnet
      121 alhcaaqygh tevvaqllqy gcdpsirnsr gesaldlaaq ygrletvqll vstypelivp
      181 lrnssssvif phtplhlasr nghravvevl laagvdvntr tsagtamhea alcgkmevvr
      241 alldrgvdlg irdsrqntvl dllgqfpphv tqditavikr hrsssgvesd adsenlppip
      301 vqggdslgsp yenvrpgddl spaegtsptq wqfyhkprdd drrvsgtsvm slgsdsglyq
      361 tppvprgtme gsfvsedlsl tlptgygggr esdqlsvsss ssvggpsprd rrclpndssa
      421 agiylpmapl ssslhspasn skvsptppkk pprrnlsvsp thlqtqmsls adctlgpsny
      481 eylfmarsga rshidleqlq krreqlrhvt rsvdqyvemk srvpdgeerr danvepvait
      541 siyenrpikt lnprrklrrh afdnyeaess pcadkspcse tdmfaqeqtf vsnafltlks
      601 sqeslldekr dtvdeqsvpe greatdkrlk rvqmmlptsp thyaqpptpd hpppsamqae
      661 ksiherirpl sqvykrrsrd metetdedll qfpaggslda ssgslssvsl sdksmstdnv
      721 eeyfgdvpfa gllkgsvtae rprtlrrlrn vygpaacgmg sggegggger ledgevpfrl
      781 sdrdrerdek slsimspfde qeewakisei masfgtglvr esvfvtelek efqtrlglss
      841 dtssspivst svgqwlstlg ladyetlfin ygfddldfin gvldeadlkd mgissdqera
      901 vimdaaglln krvdkrsggn sqsvdewlki ihlenyaetf kkhlytdmdr vrrvwevela
      961 avleiqkpah rkrilasvsg ssaraqnrns sgpnledlnk dlntlktniq qlkeeirekl
     1021 petttegngg tsiagtnstt tgtlrhrknr papqppqrps shngaisape qleirapsel
     1081 llgvpstlkt qwrhqpkalv tgcvtyvany lgstvvkelr gtestkksiq klkktcrepr
     1141 vtpditlais yrgvrflntv tnepicehei rnihcacqda ddlthfayit kdhasrthfc
     1201 hvfcvptmdq ateviltlgq afevayqmal rdklgghtvr sqsanqlttl skspasikms
     1261 ispdstpdgn lvdqpaaavt nanltcpnpk lkprskssip npivqsvvps lahdmnsnss
     1321 sgssaianct sssnpatsps tnsvsssnsn tstnqtakpl vthgrshsvn dikingnqlk
     1381 laptpiddig igvkdmkpgs rapialseel
//
LOCUS       XP_076546559             575 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane 7 superfamily member 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546559
VERSION     XP_076546559.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690444.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="transmembrane 7 superfamily member 3 isoform X1"
                     /calculated_mol_wt=63537
     Region          292..481
                     /region_name="DUF4203"
                     /note="Domain of unknown function (DUF4203); pfam13886"
                     /db_xref="CDD:464018"
     CDS             1..575
                     /gene="LOC117610813"
                     /coded_by="XM_076690444.1:150..1877"
                     /db_xref="GeneID:117610813"
ORIGIN      
        1 mnrsfvrkll ifllllancv aavkklevyd ddsvleinlr nlrdsipyik qiyispssnv
       61 tlsitnissn iafyivqiha hlhpvtlsyd anyktsiygs niglyvkstf nkvstlffkn
      121 eesyaikaml vimgyndkap ipggcnmefe teiapyakvl tsdtmivvdv qaaslpling
      181 taqtcekspv tlemyqvflp eqdrsvetyf syitsmltas sikehgqeit ssvlsnpmrr
      241 ifnaytgtgs vyvavanygk ysaayvpals ygcnpvgdpe scqvlndsys kfvcaccffi
      301 glfsvfcghk cpivdktvpv fftatiigyi aagqsitagw viglviaavy glcqhflvfr
      361 lllvltlgsf fgcvayfhfp esfiitqsdg afwalfatia vsvsiiklil ysctrgllsc
      421 aiigsymiil pfdyytgsal kyiiiniarr ctvsdfrfai iqppvqskdi tlivlwtlla
      481 iwrclkgfsv pilcqpsnve seetthllge ptapsapsip tlnsfsqsyh gyrgshrgrk
      541 hstylhimrh mlqckvtrvc trpkspgttk llndg
//
LOCUS       XP_034194537             547 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane 7 superfamily member 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194537
VERSION     XP_034194537.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338646.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194537.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..547
                     /product="transmembrane 7 superfamily member 3 isoform X2"
                     /calculated_mol_wt=60383
     Region          292..481
                     /region_name="DUF4203"
                     /note="Domain of unknown function (DUF4203); pfam13886"
                     /db_xref="CDD:464018"
     CDS             1..547
                     /gene="LOC117610813"
                     /coded_by="XM_034338646.2:149..1792"
                     /db_xref="GeneID:117610813"
ORIGIN      
        1 mnrsfvrkll ifllllancv aavkklevyd ddsvleinlr nlrdsipyik qiyispssnv
       61 tlsitnissn iafyivqiha hlhpvtlsyd anyktsiygs niglyvkstf nkvstlffkn
      121 eesyaikaml vimgyndkap ipggcnmefe teiapyakvl tsdtmivvdv qaaslpling
      181 taqtcekspv tlemyqvflp eqdrsvetyf syitsmltas sikehgqeit ssvlsnpmrr
      241 ifnaytgtgs vyvavanygk ysaayvpals ygcnpvgdpe scqvlndsys kfvcaccffi
      301 glfsvfcghk cpivdktvpv fftatiigyi aagqsitagw viglviaavy glcqhflvfr
      361 lllvltlgsf fgcvayfhfp esfiitqsdg afwalfatia vsvsiiklil ysctrgllsc
      421 aiigsymiil pfdyytgsal kyiiiniarr ctvsdfrfai iqppvqskdi tlivlwtlla
      481 iwrclkgfsv pilcqpsnve seetthllge ptapsapsip tlnsfsqsyh gyrgshrgrk
      541 hstylhi
//
LOCUS       XP_034194545             514 aa            linear   INV 03-SEP-2025
DEFINITION  paired box 6 protein twin of eyeless isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194545
VERSION     XP_034194545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338654.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="paired box 6 protein twin of eyeless isoform X1"
                     /calculated_mol_wt=54798
     Region          34..158
                     /region_name="PAX"
                     /note="Paired Box domain; smart00351"
                     /db_xref="CDD:128645"
     Site            order(39..40,45,47..49,51,56,68,70,79,82,84..85,89,
                     98..104,106..109,128..130,149,151..152,155,158)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238076"
     Region          281..336
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..514
                     /gene="toy"
                     /coded_by="XM_034338654.2:230..1774"
                     /db_xref="GeneID:117610821"
ORIGIN      
        1 mphkeedlmh gggagigggs mvgqnsifgc saaghsgvnq lggvyvngrp lpdstrqkiv
       61 elahsgarpc disrilqvsn gcvskilgry yetgsikpra iggskprvat tpvvnkiady
      121 krecpsifaw eirdrllqeg vcnndnipsv ssinrvlrnl asqkeqqaaa vqahqgaesv
      181 ydklrmfngq aagwppawys atpshhplat giptaatpgs gqsllpgsql hgrddsllkr
      241 sdtgsllshq qettsdgnse hnssgdedsq vrlrlkrklq rnrtsfsneq idslekefer
      301 thypdvfare rlaekiglpe ariqvwfsnr rakwrreekl rnqrraavdq vvgggssggs
      361 sstappaatp atprlplnag fnamyssipq piatmpdtys smssslggsm ggsclqqrae
      421 gypayvfhep lhsltqsysh ahstaahsqp hrgiptshgg tpttpggqpa gpggapyqpt
      481 tstatgvisa gvsvpvqips qtpdltgnyw prlq
//
LOCUS       XP_034194549             510 aa            linear   INV 03-SEP-2025
DEFINITION  paired box 6 protein twin of eyeless isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194549
VERSION     XP_034194549.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338658.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="paired box 6 protein twin of eyeless isoform X2"
                     /calculated_mol_wt=54330
     Region          34..158
                     /region_name="PAX"
                     /note="Paired Box domain; smart00351"
                     /db_xref="CDD:128645"
     Site            order(39..40,45,47..49,51,56,68,70,79,82,84..85,89,
                     98..104,106..109,128..130,149,151..152,155,158)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238076"
     Region          281..332
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..510
                     /gene="toy"
                     /coded_by="XM_034338658.2:230..1762"
                     /db_xref="GeneID:117610821"
ORIGIN      
        1 mphkeedlmh gggagigggs mvgqnsifgc saaghsgvnq lggvyvngrp lpdstrqkiv
       61 elahsgarpc disrilqvsn gcvskilgry yetgsikpra iggskprvat tpvvnkiady
      121 krecpsifaw eirdrllqeg vcnndnipsv ssinrvlrnl asqkeqqaaa vqahqgaesv
      181 ydklrmfngq aagwppawys atpshhplat giptaatpgs gqsllpgsql hgrddsllkr
      241 sdtgsllshq qettsdgnse hnssgdedsq vrlrlkrklq rnrtsfsneq idslekefer
      301 thypdvfare rlaekiglpe vwfsnrrakw rreeklrnqr raavdqvvgg gssggsssta
      361 ppaatpatpr lplnagfnam yssipqpiat mpdtyssmss slggsmggsc lqqraegypa
      421 yvfheplhsl tqsyshahst aahsqphrgi ptshggtptt pggqpagpgg apyqpttsta
      481 tgvisagvsv pvqipsqtpd ltgnywprlq
//
LOCUS       XP_034194558             457 aa            linear   INV 03-SEP-2025
DEFINITION  tetratricopeptide repeat protein 5 [Osmia lignaria lignaria].
ACCESSION   XP_034194558
VERSION     XP_034194558.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338667.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194558.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="tetratricopeptide repeat protein 5"
                     /calculated_mol_wt=51695
     Region          78..>209
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          86..117
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          122..150
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          156..192
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          184..>273
                     /region_name="NrfG"
                     /note="Cytochrome c-type biogenesis protein CcmH/NrfG
                     [Energy production and conversion, Posttranslational
                     modification, protein turnover, chaperones]; COG4235"
                     /db_xref="CDD:443378"
     Region          197..237
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          242..269
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          335..448
                     /region_name="TTC5_OB"
                     /note="Tetratricopeptide repeat protein 5 OB fold domain;
                     pfam16669"
                     /db_xref="CDD:465225"
     CDS             1..457
                     /gene="LOC117610834"
                     /coded_by="XM_034338667.2:40..1413"
                     /db_xref="GeneID:117610834"
ORIGIN      
        1 mkvfrkmsai iddkvvagak sansvkedpi valtekvnal yffrdhyfvn hsieeaikkk
       61 sdvekemkdt inkfdeckgy eidgsrakyy ylkgkalnvt erfipqaeel ltkavklepn
      121 lveawnelge cywknddiqq akncfvgalr hgrnkvslrn lsmvlrqesv stveqqiqni
      181 qkgveyakea vsldttdgis wailgnayly sfftvaqnps tlrlcmsayv qaekdivags
      241 npdlfhnrav alkyqeeycl alksferaml ldplwetphn krdellqylk dvqnlvnnng
      301 kvkpkrlyqm iraldvkhlg pykggsftsg qktvkldlvs lktltlglnp ekvifgkvvc
      361 wiqdtdcvpf afclvdeekt cigvtvynla kgrgvtvgds vaipepfvih qkfsyldndf
      421 dfksirvetp vilvvngrkl greqqasakl htfkktd
//
LOCUS       XP_034194565             433 aa            linear   INV 03-SEP-2025
DEFINITION  alg3, alpha-1,3- mannosyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034194565
VERSION     XP_034194565.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338674.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="alg3, alpha-1,3- mannosyltransferase"
                     /calculated_mol_wt=50421
     Region          50..411
                     /region_name="ALG3"
                     /note="ALG3 protein; pfam05208"
                     /db_xref="CDD:461587"
     CDS             1..433
                     /gene="Alg3"
                     /coded_by="XM_034338674.2:295..1596"
                     /db_xref="GeneID:117610842"
ORIGIN      
        1 maprrelrsn lnsaktlkkn tknwyenyle wekfvslftd prkliiigrv fivleiilnv
       61 lviekvpyte idwkaymqev egflngtldy sklrgdtgpl vypagfvyif silhyitgrg
      121 akikiaqysf aalyiilltl vfriyaktkk vppyvliimc ctsyrvhsif tlrlfndpva
      181 mvllfaslna fldnhwylgs ilyslavsvk mnillfapal lvaylcnlgl ikatvhlfic
      241 afiqlilglp fllnnplayi kgafdlgrvf efkwtvnwrf lsdnvfihpy fhvlllvlhi
      301 ltiiyciptw ikymksyakl kyieenvqsq lkkkekvdms tmsqlfifpf fvanfigiaf
      361 srslhyqfyi wyyhtlpyia wctdyktvik ltilgiielc wntypstils svllhichlm
      421 llygllkdks kne
//
LOCUS       XP_034194571             399 aa            linear   INV 03-SEP-2025
DEFINITION  membrane-attack complex domain containing protein torso-like
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194571
VERSION     XP_034194571.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338680.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="membrane-attack complex domain containing
                     protein torso-like isoform X1"
                     /calculated_mol_wt=46186
     Region          241..>322
                     /region_name="MACPF"
                     /note="membrane-attack complex / perforin; cl02616"
                     /db_xref="CDD:470633"
     CDS             1..399
                     /gene="tsl"
                     /coded_by="XM_034338680.2:9502..10701"
                     /db_xref="GeneID:117610846"
ORIGIN      
        1 mvifhvaair cfiiararkr avlrsrrftr asssllkrpn ivstkrwfsr rqpremwsrt
       61 rflvlasmla llvgarvsng sqrprlggav nifsrygyls ismrvvprnd tdtwifrept
      121 ldvfrnpvpv asrqrqqaaa vfdgdfhmef cdnirqlmqa yfrdftferl erpwraftgs
      181 wskaaiarhl ginssfitgd hcyvlvrvar frdnqklaat adsmildeav lretenvtvg
      241 dtasvvrfik hfgshyiaay vtgnslyqvf vytpqaylrl kerlktrgva dlsnrelsnf
      301 fspwyaehmg siqaasgnrt veawaverlr nqyyifsyas llklhgdall lkqldglled
      361 eallqlqlrt lapifkdpqr rdwflevidn yfklwevnm
//
LOCUS       XP_034194577             344 aa            linear   INV 03-SEP-2025
DEFINITION  membrane-attack complex domain containing protein torso-like
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194577
VERSION     XP_034194577.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338686.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..344
                     /product="membrane-attack complex domain containing
                     protein torso-like isoform X2"
                     /calculated_mol_wt=39606
     Region          186..>267
                     /region_name="MACPF"
                     /note="membrane-attack complex / perforin; cl02616"
                     /db_xref="CDD:470633"
     CDS             1..344
                     /gene="tsl"
                     /coded_by="XM_034338686.2:522..1556"
                     /db_xref="GeneID:117610846"
ORIGIN      
        1 mwsrtrflvl asmlallvga rvsngsqrpr lggavnifsr ygylsismrv vprndtdtwi
       61 freptldvfr npvpvasrqr qqaaavfdgd fhmefcdnir qlmqayfrdf tferlerpwr
      121 aftgswskaa iarhlginss fitgdhcyvl vrvarfrdnq klaatadsmi ldeavlrete
      181 nvtvgdtasv vrfikhfgsh yiaayvtgns lyqvfvytpq aylrlkerlk trgvadlsnr
      241 elsnffspwy aehmgsiqaa sgnrtveawa verlrnqyyi fsyasllklh gdalllkqld
      301 glledeallq lqlrtlapif kdpqrrdwfl evidnyfklw evnm
//
LOCUS       XP_034194580             122 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin like 3 [Osmia lignaria lignaria].
ACCESSION   XP_034194580
VERSION     XP_034194580.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338689.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..122
                     /product="ubiquitin like 3"
                     /calculated_mol_wt=13265
     Region          11..92
                     /region_name="Ubl_UBL3"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin-like
                     protein 3 (UBL3) and similar proteins; cd17048"
                     /db_xref="CDD:340568"
     CDS             1..122
                     /gene="UBL3"
                     /coded_by="XM_034338689.2:374..742"
                     /db_xref="GeneID:117610857"
ORIGIN      
        1 mssrnipsdk inlrlilvsg ktkeflfsps dsagdiahhv fenwpedwae eavakaeilr
       61 liyqgrflhs nvtlgalglp fgkttvmhlv prenlpepns qdqrqkskgg gssccsascc
      121 il
//
LOCUS       XP_034195752             365 aa            linear   INV 03-SEP-2025
DEFINITION  protein mab-21 [Osmia lignaria lignaria].
ACCESSION   XP_034195752
VERSION     XP_034195752.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339861.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="protein mab-21"
                     /calculated_mol_wt=41473
     Region          68..250
                     /region_name="Mab-21"
                     /note="Mab-21 protein nucleotidyltransferase domain;
                     pfam03281"
                     /db_xref="CDD:460873"
     Region          253..350
                     /region_name="Mab-21_C"
                     /note="Mab-21 protein HhH/H2TH-like domain; pfam20266"
                     /db_xref="CDD:466416"
     CDS             1..365
                     /gene="LOC117611713"
                     /coded_by="XM_034339861.2:338..1435"
                     /db_xref="GeneID:117611713"
ORIGIN      
        1 mllpadmlaa qskmvyqink yfgervmtrk sqvmktiqev crvvqdvlke vevqeprfis
       61 sltdyngrfd gldvisptef eiviylnqmg vlnfvddgtl pgcavlklsd grkrsmslwv
      121 efitasgyls arkirsrfqt lvaqacdkca yrdsvkmiad ttevklrire ryvvqitpaf
      181 kcaglwprsa shwpiahisw phpnivaevk tegfdmlske ciglqgkqsa megdawalsf
      241 idaenrllqg gsrkrclsil ktlrdrhldl pgnpvtsyhm ktlllyecek hpheaewdet
      301 cigdringil lqlisclqcr rcphyflpnl dlfkgkspsg lenaakqvwr ltrelltnsr
      361 alekl
//
LOCUS       XP_034195753             365 aa            linear   INV 03-SEP-2025
DEFINITION  protein mab-21 [Osmia lignaria lignaria].
ACCESSION   XP_034195753
VERSION     XP_034195753.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339862.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="protein mab-21"
                     /calculated_mol_wt=41566
     Region          68..250
                     /region_name="Mab-21"
                     /note="Mab-21 protein nucleotidyltransferase domain;
                     pfam03281"
                     /db_xref="CDD:460873"
     Region          252..350
                     /region_name="Mab-21_C"
                     /note="Mab-21 protein HhH/H2TH-like domain; pfam20266"
                     /db_xref="CDD:466416"
     CDS             1..365
                     /gene="LOC117611714"
                     /coded_by="XM_034339862.2:384..1481"
                     /db_xref="GeneID:117611714"
ORIGIN      
        1 mllpqdmmaa qskmlyqmnk yygervqarm gqvqktirev ckvvqdvlke vevqeprfis
       61 sltecngrye glevispgef evvlylnqmg vfnfvddgtl pgcavlklsd grkrsmslwv
      121 efitasgyls arkirsrfqt lvaqacdkca yrdsvkmiad ttevklrire ryvvqitpaf
      181 kcsgvwprsa ahwpiphipw phpnlvaevk tegfdllske svalqgkqsa megdawvlsf
      241 teaetrllqg gcrrrclsil ktlrdrhldl pgnpvtsyhm ktlllyecek hpletewdeg
      301 cladringif lqlisclqcr rcphyflpnl dlfkgkspsg lenaakqvwr ltrelltnsr
      361 alekl
//
LOCUS       XP_076546560             998 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein WDY isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546560
VERSION     XP_076546560.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690445.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..998
                     /product="WD repeat-containing protein WDY isoform X1"
                     /calculated_mol_wt=114363
     Region          <63..94
                     /region_name="EF-hand_8"
                     /note="EF-hand domain pair; pfam13833"
                     /db_xref="CDD:404678"
     Region          186..417
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          348..642
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          350..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(363,367,373..374,387..388,406,410,416..417,436..437,
                     454,463..464,477,494,499,505..506,526,529,547,551,
                     557..558,570..571,588,593,599..600,614,631,635,641..642)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          392..428
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          440..474
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          481..526
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          529..868
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(529,547,551,557..558,570..571,588,592,598,602,614,
                     630,635,641..642,666,672..673,697..698,731,735,741..742,
                     776,781,787,818,839..840,858,862,868)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          533..569
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          618..643
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          661..692
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          709..754
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          763..838
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          844..868
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..998
                     /gene="WDY"
                     /coded_by="XM_076690445.1:140..3136"
                     /db_xref="GeneID:117611019"
ORIGIN      
        1 menldsnisv qqafdnffkt ksieeqctee llirlheafl iseneemnst qlfgafenil
       61 hirmpkdefe vlfkkmnlkr dgnvswnefi syllvefqkk dnalqwkilk lpitgipqll
      121 ksrhrtlihk ivfcpellsn rtasfhrgsy ltitregiin ywsldleyer tvqsvnpylk
      181 vqstlitdmi vmpdvqvvct ssteydlrfy dtaakkfdlr ilisgldyai tcmdyyfskn
      241 vkedshivcg dmsgsikiis fnptergpfk qepqchtlfi ryesilkdev kgmkiiefkn
      301 mhtdwvkqva yyeslrsfms ssrccscsll vsdltgsriq ykfkvnmgis cftfceesqs
      361 lvtggpdciv riwnpfvtkr pnckfqghht ticalvvqns gkrvyslskd kcikvwdvla
      421 qsciqtyngl pselgghtlm svvyntlnhk mivastmiai iicdpvinee tsdgfthtkp
      481 vscvlynhly kvvvttglds ciivwdpwlg nrlylvthah srflygqhhd ieitaacfde
      541 seqllvtgtr dgtlkiwnfn tgtclrnmtv enqceitsla wvenrilcsg wnrqvvefat
      601 sdtdiykksw glkhtddilc savwypqvla tatyngeiil wrletgqpyr kykvddpmsr
      661 fsiryemtek mielnklqqf rtekfahkks kshaaskhqe salsiigtva vramiflcar
      721 sveynigtll vsldsgliqt wthhpaggfl qafsvihtlg dcalslatdp knhflvtghs
      781 agyikvwyle nylvphppki cmpllrlefp flwkskingr akravrnqpl plllssihgh
      841 lkaitsiqii pdariiisgs adhtvrlwtl ggryistfgt frpwspilpt ipvyhyfeyy
      901 rlppdvkrfg ssttmkvlgg daektiseyk ddttdilkhi perkqttidg rkltisvidr
      961 sllmifqdyp vldtslpyip iythlklrpl eiiqmprl
//
LOCUS       XP_076546561             997 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein WDY isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546561
VERSION     XP_076546561.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690446.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..997
                     /product="WD repeat-containing protein WDY isoform X2"
                     /calculated_mol_wt=114264
     Region          <63..94
                     /region_name="EF-hand_8"
                     /note="EF-hand domain pair; pfam13833"
                     /db_xref="CDD:404678"
     Region          186..417
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          348..641
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          350..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(363,367,373..374,387..388,406,410,416..417,436..437,
                     454,463..464,477,494,498,504..505,525,528,546,550,
                     556..557,569..570,587,592,598..599,613,630,634,640..641)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          392..428
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          440..474
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          481..525
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          528..867
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(528,546,550,556..557,569..570,587,591,597,601,613,
                     629,634,640..641,665,671..672,696..697,730,734,740..741,
                     775,780,786,817,838..839,857,861,867)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          532..568
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          617..642
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          660..691
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          708..753
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          762..837
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          843..867
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..997
                     /gene="WDY"
                     /coded_by="XM_076690446.1:140..3133"
                     /db_xref="GeneID:117611019"
ORIGIN      
        1 menldsnisv qqafdnffkt ksieeqctee llirlheafl iseneemnst qlfgafenil
       61 hirmpkdefe vlfkkmnlkr dgnvswnefi syllvefqkk dnalqwkilk lpitgipqll
      121 ksrhrtlihk ivfcpellsn rtasfhrgsy ltitregiin ywsldleyer tvqsvnpylk
      181 vqstlitdmi vmpdvqvvct ssteydlrfy dtaakkfdlr ilisgldyai tcmdyyfskn
      241 vkedshivcg dmsgsikiis fnptergpfk qepqchtlfi ryesilkdev kgmkiiefkn
      301 mhtdwvkqva yyeslrsfms ssrccscsll vsdltgsriq ykfkvnmgis cftfceesqs
      361 lvtggpdciv riwnpfvtkr pnckfqghht ticalvvqns gkrvyslskd kcikvwdvla
      421 qsciqtyngl pselgghtlm svvyntlnhk mivastmiai iicdpvinee tsdgfthtkp
      481 vscvlynhly kvvttgldsc iivwdpwlgn rlylvthahs rflygqhhdi eitaacfdes
      541 eqllvtgtrd gtlkiwnfnt gtclrnmtve nqceitslaw venrilcsgw nrqvvefats
      601 dtdiykkswg lkhtddilcs avwypqvlat atyngeiilw rletgqpyrk ykvddpmsrf
      661 siryemtekm ielnklqqfr tekfahkksk shaaskhqes alsiigtvav ramiflcars
      721 veynigtllv sldsgliqtw thhpaggflq afsvihtlgd calslatdpk nhflvtghsa
      781 gyikvwylen ylvphppkic mpllrlefpf lwkskingra kravrnqplp lllssihghl
      841 kaitsiqiip dariiisgsa dhtvrlwtlg gryistfgtf rpwspilpti pvyhyfeyyr
      901 lppdvkrfgs sttmkvlggd aektiseykd dttdilkhip erkqttidgr kltisvidrs
      961 llmifqdypv ldtslpyipi ythlklrple iiqmprl
//
LOCUS       XP_076546562             986 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein WDY isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546562
VERSION     XP_076546562.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690447.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..986
                     /product="WD repeat-containing protein WDY isoform X3"
                     /calculated_mol_wt=112943
     Region          <63..94
                     /region_name="EF-hand_8"
                     /note="EF-hand domain pair; pfam13833"
                     /db_xref="CDD:404678"
     Region          186..417
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          348..630
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          350..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(363,367,373..374,387..388,406,410,416..417,436..437,
                     454,463..464,477,483,487,493..494,514,517,535,539,
                     545..546,558..559,576,581,587..588,602,619,623,629..630)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          392..428
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          440..475
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          515..>872
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          521..557
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          606..631
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          708..742
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          751..826
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          832..856
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..986
                     /gene="WDY"
                     /coded_by="XM_076690447.1:140..3100"
                     /db_xref="GeneID:117611019"
ORIGIN      
        1 menldsnisv qqafdnffkt ksieeqctee llirlheafl iseneemnst qlfgafenil
       61 hirmpkdefe vlfkkmnlkr dgnvswnefi syllvefqkk dnalqwkilk lpitgipqll
      121 ksrhrtlihk ivfcpellsn rtasfhrgsy ltitregiin ywsldleyer tvqsvnpylk
      181 vqstlitdmi vmpdvqvvct ssteydlrfy dtaakkfdlr ilisgldyai tcmdyyfskn
      241 vkedshivcg dmsgsikiis fnptergpfk qepqchtlfi ryesilkdev kgmkiiefkn
      301 mhtdwvkqva yyeslrsfms ssrccscsll vsdltgsriq ykfkvnmgis cftfceesqs
      361 lvtggpdciv riwnpfvtkr pnckfqghht ticalvvqns gkrvyslskd kcikvwdvla
      421 qsciqtyngl pselgghtlm svvyntlnhk mivastmiai iicdpvinee tsdgfthtkp
      481 vvttgldsci ivwdpwlgnr lylvthahsr flygqhhdie itaacfdese qllvtgtrdg
      541 tlkiwnfntg tclrnmtven qceitslawv enrilcsgwn rqvvefatsd tdiykkswgl
      601 khtddilcsa vwypqvlata tyngeiilwr letgqpyrky kvddpmsrfs iryemtekmi
      661 elnklqqfrt ekfahkksks haaskhqesa lsiigtvavr amiflcarsv eynigtllvs
      721 ldsgliqtwt hhpaggflqa fsvihtlgdc alslatdpkn hflvtghsag yikvwyleny
      781 lvphppkicm pllrlefpfl wkskingrak ravrnqplpl llssihghlk aitsiqiipd
      841 ariiisgsad htvrlwtlgg ryistfgtfr pwspilptip vyhyfeyyrl ppdvkrfgss
      901 ttmkvlggda ektiseykdd ttdilkhipe rkqttidgrk ltisvidrsl lmifqdypvl
      961 dtslpyipiy thlklrplei iqmprl
//
LOCUS       XP_076546563             970 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein WDY isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546563
VERSION     XP_076546563.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690448.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..970
                     /product="WD repeat-containing protein WDY isoform X4"
                     /calculated_mol_wt=111226
     Region          <35..66
                     /region_name="EF-hand_8"
                     /note="EF-hand domain pair; pfam13833"
                     /db_xref="CDD:404678"
     Region          158..389
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          320..614
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          322..359
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(335,339,345..346,359..360,378,382,388..389,408..409,
                     426,435..436,449,466,471,477..478,498,501,519,523,
                     529..530,542..543,560,565,571..572,586,603,607,613..614)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          364..400
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          412..446
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          453..498
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          501..840
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(501,519,523,529..530,542..543,560,564,570,574,586,
                     602,607,613..614,638,644..645,669..670,703,707,713..714,
                     748,753,759,790,811..812,830,834,840)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          505..541
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          590..615
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          633..664
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          681..726
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          735..810
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          816..840
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..970
                     /gene="WDY"
                     /coded_by="XM_076690448.1:211..3123"
                     /db_xref="GeneID:117611019"
ORIGIN      
        1 myrrtfnsfa rsifeneemn stqlfgafen ilhirmpkde fevlfkkmnl krdgnvswne
       61 fisyllvefq kkdnalqwki lklpitgipq llksrhrtli hkivfcpell snrtasfhrg
      121 syltitregi inywsldley ertvqsvnpy lkvqstlitd mivmpdvqvv ctssteydlr
      181 fydtaakkfd lrilisgldy aitcmdyyfs knvkedshiv cgdmsgsiki isfnptergp
      241 fkqepqchtl firyesilkd evkgmkiief knmhtdwvkq vayyeslrsf msssrccscs
      301 llvsdltgsr iqykfkvnmg iscftfcees qslvtggpdc ivriwnpfvt krpnckfqgh
      361 htticalvvq nsgkrvysls kdkcikvwdv laqsciqtyn glpselgght lmsvvyntln
      421 hkmivastmi aiiicdpvin eetsdgftht kpvscvlynh lykvvvttgl dsciivwdpw
      481 lgnrlylvth ahsrflygqh hdieitaacf deseqllvtg trdgtlkiwn fntgtclrnm
      541 tvenqceits lawvenrilc sgwnrqvvef atsdtdiykk swglkhtddi lcsavwypqv
      601 latatyngei ilwrletgqp yrkykvddpm srfsiryemt ekmielnklq qfrtekfahk
      661 kskshaaskh qesalsiigt vavramiflc arsveynigt llvsldsgli qtwthhpagg
      721 flqafsviht lgdcalslat dpknhflvtg hsagyikvwy lenylvphpp kicmpllrle
      781 fpflwkskin grakravrnq plplllssih ghlkaitsiq iipdariiis gsadhtvrlw
      841 tlggryistf gtfrpwspil ptipvyhyfe yyrlppdvkr fgssttmkvl ggdaektise
      901 ykddttdilk hiperkqtti dgrkltisvi drsllmifqd ypvldtslpy ipiythlklr
      961 pleiiqmprl
//
LOCUS       XP_076546564             968 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein WDY isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546564
VERSION     XP_076546564.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690449.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..968
                     /product="WD repeat-containing protein WDY isoform X5"
                     /calculated_mol_wt=110549
     Region          <63..94
                     /region_name="EF-hand_8"
                     /note="EF-hand domain pair; pfam13833"
                     /db_xref="CDD:404678"
     Region          186..417
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          348..642
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          350..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(363,367,373..374,387..388,406,410,416..417,436..437,
                     454,463..464,477,494,499,505..506,526,529,547,551,
                     557..558,570..571,588,593,599..600,614,631,635,641..642)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          392..428
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          440..474
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          481..526
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          529..838
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          533..569
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          618..643
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          685..724
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          732..806
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          814..840
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..968
                     /gene="WDY"
                     /coded_by="XM_076690449.1:140..3046"
                     /db_xref="GeneID:117611019"
ORIGIN      
        1 menldsnisv qqafdnffkt ksieeqctee llirlheafl iseneemnst qlfgafenil
       61 hirmpkdefe vlfkkmnlkr dgnvswnefi syllvefqkk dnalqwkilk lpitgipqll
      121 ksrhrtlihk ivfcpellsn rtasfhrgsy ltitregiin ywsldleyer tvqsvnpylk
      181 vqstlitdmi vmpdvqvvct ssteydlrfy dtaakkfdlr ilisgldyai tcmdyyfskn
      241 vkedshivcg dmsgsikiis fnptergpfk qepqchtlfi ryesilkdev kgmkiiefkn
      301 mhtdwvkqva yyeslrsfms ssrccscsll vsdltgsriq ykfkvnmgis cftfceesqs
      361 lvtggpdciv riwnpfvtkr pnckfqghht ticalvvqns gkrvyslskd kcikvwdvla
      421 qsciqtyngl pselgghtlm svvyntlnhk mivastmiai iicdpvinee tsdgfthtkp
      481 vscvlynhly kvvvttglds ciivwdpwlg nrlylvthah srflygqhhd ieitaacfde
      541 seqllvtgtr dgtlkiwnfn tgtclrnmtv enqceitsla wvenrilcsg wnrqvvefat
      601 sdtdiykksw glkhtddilc savwypqvla tatyngeiil wrletgqpyr kykvddpmss
      661 kshaaskhqe salsiigtva vramiflcar sveynigtll vsldsgliqt wthhpaggfl
      721 qafsvihtlg dcalslatdp knhflvtghs agyikvwyle nylvphppki cmpllrlefp
      781 flwkskingr akravrnqpl plllssihgh lkaitsiqii pdariiisgs adhtvrlwtl
      841 ggryistfgt frpwspilpt ipvyhyfeyy rlppdvkrfg ssttmkvlgg daektiseyk
      901 ddttdilkhi perkqttidg rkltisvidr sllmifqdyp vldtslpyip iythlklrpl
      961 eiiqmprl
//
LOCUS       XP_034194806             874 aa            linear   INV 03-SEP-2025
DEFINITION  inner centromere protein [Osmia lignaria lignaria].
ACCESSION   XP_034194806
VERSION     XP_034194806.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338915.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194806.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..874
                     /product="inner centromere protein"
                     /calculated_mol_wt=100049
     Region          <362..760
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <616..>762
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..874
                     /gene="Incenp"
                     /coded_by="XM_034338915.2:287..2911"
                     /db_xref="GeneID:117611025"
ORIGIN      
        1 metqsrdtik amitkdfsdi nnrymevkvf ikdslkdtld ylhdiiaqip nsvsgplitk
       61 tpkvlrkkak qrietipend vvdltnmssd ctavhvtven ndittksvne tmpvrlkrea
      121 skkaadnikk qqslslntkl rrplsdheas nldvkkkkre sqtkrkistr sssdeesskg
      181 prkltkmeet ivikkvpeki sdshiekqss lkangrtskk sstatrsssr krtfsqtqdq
      241 nsnkqsniid dtiiapetne idepsmyeda igkplpimns tmnfnstytm qkmmcatvil
      301 eplsaiklne tvtvnkkknt nidgkmiqpn gefksprlss klestfsvtl qdrmvqlkea
      361 iankefdell tedesspeik krrqnakiqe ntkrqkrvtr stvssedeil stpakptlke
      421 lntgstfqev knmyksnalf spyakesvkk rveafeqvgm nspkpaidid aptrmtrtkt
      481 raqaaaqaem leiskaaekt vaqklarksi akakkislak qykdtdkenk iqakqkvlse
      541 kvkqkqqktt pltktkiqlp ssvtriphtp lnnysninhg kalsdartni itsmdsflqp
      601 pksvnkcnst gkvegkkrkl ndddakkkre evlrlvteer krkrqekelk nklareakek
      661 qdmekrqrae rereekarla qqmlekqree iekkrlaqlq ramekeekrk qeeqirlqrl
      721 qeqeeaeril aeqrrreqea ekrkeaeara qqqtaaeaik tkihihgtqt kygskhhgpt
      781 nyildsepde desddesrpk hvipywahpq vrksqlamqr yipegavykf fgsrkctpdl
      841 tklfqgidks rlkrtssaiw qtpprfsmig seml
//
LOCUS       XP_034194834            1804 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 131 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194834
VERSION     XP_034194834.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338943.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1804
                     /product="transmembrane protein 131 isoform X1"
                     /calculated_mol_wt=203231
     Region          79..162
                     /region_name="TMEM131_like_N"
                     /note="Transmembrane protein 131-like N-terminal;
                     pfam12371"
                     /db_xref="CDD:463553"
     Region          <706..>1140
                     /region_name="TMEM131_like"
                     /note="Transmembrane protein 131-like; pfam19532"
                     /db_xref="CDD:437364"
     CDS             1..1804
                     /gene="Tmem131"
                     /coded_by="XM_034338943.2:441..5855"
                     /db_xref="GeneID:117611055"
ORIGIN      
        1 mtelkvlyyl llltffelts qirpslhghn nafvqddndv qylldnipms mhkdfantvh
       61 gagdttsded kiqdslsyvh fkphvldfke rqlgiphqet vilfnrdhnk tihllsisgn
      121 trhfhasffr nkvipplgnt tfdviflgrd egdidthlfi htadgtvkyq vkgisvsspy
      181 rlrpvvgvkl plnatftpli ymhnphaeal qvlevyssgg efqlelpsge aegsrelwei
      241 ppyqtkpiir lhfnaytekn htayirlkvn ntsevlvvtv evevkngagl hwggssgvin
      301 lgmggslqpp iqypialkns akkpikimni istpvskalk lhfeqavipg dtdipiaiga
      361 liydwktgle lqhfkgklvi kaigpggssq kltipwiaqv lqgglevnas ithycslqsn
      421 qarnfsvvnk fklplaitnv tmsshvkslf tiknfiprvi kpeqkvnifs lqlakdrktd
      481 nvkmessili hsnvsvtevp llsydgkvrk ivpeeresde gtmnfgtvgs gteneaifal
      541 enqnpinidl hgwgvnmpga vlelmgcqrg padfldkelr nitvcshtgn qyikpgylai
      601 fkikvktpmv eeativgdvf vrttyerftl pvymrvahgr islkkliftd cfpgsicvqq
      661 lkvystfvrp mqvtqiapvn kdnrikyipl eeasmpvitk genyigsiri epsmackhyc
      721 ylglsldsna grqwlntlal pshtrdsdln llntrytrfl nstggrswdn itmrldttev
      781 rghkfylnik phwpslltgf gnnknkvalm fpltqvgnts ystikvynps tspliiqlvm
      841 dwnypqgarl yhslpakfkf tcvecgstia eefkleessv drekfekewd vtvipqsipl
      901 ylkplesrti rvsytpfsps lssallyirn nmtilevlrv igqgtnaqfr fgnrkpgstt
      961 pllfeladkh lkdcereqlk rnpvpnltvk rsfmarntge lpievydfyi nglrcegygf
     1021 kvlncmsfkl npnatkkvei aftpdftlsr verkllists lgsdsdieng mvilnllatl
     1081 pphsleacta altrpsweyv vhwaaislss illicilais fleadrilrg aiasfsresp
     1141 vqppldlrll shmpvhstqe stsvkeklin ddkqkimkke etypdwalmn vkkfkdkdnv
     1201 qkglkipdws adeerrfkld tetknllsfk ryeestldns niisnitlga kkknnkkqnn
     1261 vqeiqtdncv genvfpdiqg tqekkytvna faktspvtnr kgksnqggiv keetklvdhe
     1321 vqvdttglln ntraskldnk rkqttvtssn nnhvsfkkfe snssqrnnih lseeetsstt
     1381 tessvqddpp pcknfdqscg kseklqrkpv nkktkpqsia pvpcvdyrdn yegdcdddey
     1441 dkdrhnnpnr wktntrstik hhihtsrtve ssfkiprqnk npprkdkgsq krrgidkghi
     1501 ktaslngnts qredptrtlg tvstplpppp acwgenrarf sdvvarnqes ispfsglnkl
     1561 hktqtnthnl smvfnndtkd vdymkqqsgq eliqntkeyt mipkspplci qsvekekplg
     1621 qdslkiqnnt qhsnsyfina ftespferel vpyddlpetd eplmelespe edtrcqlwed
     1681 nnsmlkllsd ntsgfqless kppekplilt dtlkdnwasv etnweplytr tavgeersgv
     1741 wgvntggvwa agpwgaatpp misqlpslpt esdtqersgf dpfrslstiw tpsssecwkk
     1801 khed
//
LOCUS       XP_034194839            1803 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 131 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194839
VERSION     XP_034194839.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338948.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1803
                     /product="transmembrane protein 131 isoform X2"
                     /calculated_mol_wt=203160
     Region          79..162
                     /region_name="TMEM131_like_N"
                     /note="Transmembrane protein 131-like N-terminal;
                     pfam12371"
                     /db_xref="CDD:463553"
     Region          <706..>1140
                     /region_name="TMEM131_like"
                     /note="Transmembrane protein 131-like; pfam19532"
                     /db_xref="CDD:437364"
     CDS             1..1803
                     /gene="Tmem131"
                     /coded_by="XM_034338948.2:443..5854"
                     /db_xref="GeneID:117611055"
ORIGIN      
        1 mtelkvlyyl llltffelts qirpslhghn nafvqddndv qylldnipms mhkdfantvh
       61 gagdttsded kiqdslsyvh fkphvldfke rqlgiphqet vilfnrdhnk tihllsisgn
      121 trhfhasffr nkvipplgnt tfdviflgrd egdidthlfi htadgtvkyq vkgisvsspy
      181 rlrpvvgvkl plnatftpli ymhnphaeal qvlevyssgg efqlelpsge aegsrelwei
      241 ppyqtkpiir lhfnaytekn htayirlkvn ntsevlvvtv evevkngagl hwggssgvin
      301 lgmggslqpp iqypialkns akkpikimni istpvskalk lhfeqavipg dtdipiaiga
      361 liydwktgle lqhfkgklvi kaigpggssq kltipwiaqv lqgglevnas ithycslqsn
      421 qarnfsvvnk fklplaitnv tmsshvkslf tiknfiprvi kpeqkvnifs lqlakdrktd
      481 nvkmessili hsnvsvtevp llsydgkvrk ivpeeresde gtmnfgtvgs gteneaifal
      541 enqnpinidl hgwgvnmpga vlelmgcqrg padfldkelr nitvcshtgn qyikpgylai
      601 fkikvktpmv eeativgdvf vrttyerftl pvymrvahgr islkkliftd cfpgsicvqq
      661 lkvystfvrp mqvtqiapvn kdnrikyipl eeasmpvitk genyigsiri epsmackhyc
      721 ylglsldsna grqwlntlal pshtrdsdln llntrytrfl nstggrswdn itmrldttev
      781 rghkfylnik phwpslltgf gnnknkvalm fpltqvgnts ystikvynps tspliiqlvm
      841 dwnypqgarl yhslpakfkf tcvecgstia eefkleessv drekfekewd vtvipqsipl
      901 ylkplesrti rvsytpfsps lssallyirn nmtilevlrv igqgtnaqfr fgnrkpgstt
      961 pllfeladkh lkdcereqlk rnpvpnltvk rsfmarntge lpievydfyi nglrcegygf
     1021 kvlncmsfkl npnatkkvei aftpdftlsr verkllists lgsdsdieng mvilnllatl
     1081 pphsleacta altrpsweyv vhwaaislss illicilais fleadrilrg aiasfsresp
     1141 vqppldlrll shmpvhstqe stsvkeklin ddkqkimkke etypdwalmn vkkfkdkdnv
     1201 qkglkipdws adeerrfkld tetknllsfk ryeestldns niisnitlga kkknnkkqnn
     1261 vqeiqtdncv genvfpdiqg tqekkytvna faktspvtnr kgksnqggiv keetklvdhe
     1321 vqvdttglln ntraskldnk rkqttvtssn nnhvsfkkfe snssqrnnih lseeetsstt
     1381 tessvqddpp pcknfdqscg kseklqrkpv nkktkpqsia pvpcvdyrdn yegdcdddey
     1441 dkdrhnnpnr wktntrstik hhihtsrtve ssfkiprqnk npprkdkgsq krrgidkghi
     1501 ktslngntsq redptrtlgt vstplppppa cwgenrarfs dvvarnqesi spfsglnklh
     1561 ktqtnthnls mvfnndtkdv dymkqqsgqe liqntkeytm ipkspplciq svekekplgq
     1621 dslkiqnntq hsnsyfinaf tespferelv pyddlpetde plmelespee dtrcqlwedn
     1681 nsmlkllsdn tsgfqlessk ppekpliltd tlkdnwasve tnweplytrt avgeersgvw
     1741 gvntggvwaa gpwgaatppm isqlpslpte sdtqersgfd pfrslstiwt psssecwkkk
     1801 hed
//
LOCUS       XP_034194862            1565 aa            linear   INV 03-SEP-2025
DEFINITION  DENN domain-containing protein Crag isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194862
VERSION     XP_034194862.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338971.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1565
                     /product="DENN domain-containing protein Crag isoform X1"
                     /calculated_mol_wt=177007
     Region          50..>214
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     Region          198..281
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          355..538
                     /region_name="DENN"
                     /note="DENN (AEX-3) domain; pfam02141"
                     /db_xref="CDD:460461"
     Region          607..680
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          846..875
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          847..884
                     /region_name="PPR_2"
                     /note="PPR repeat family; pfam13041"
                     /db_xref="CDD:463778"
     CDS             1..1565
                     /gene="Crag"
                     /coded_by="XM_034338971.2:427..5124"
                     /db_xref="GeneID:117611071"
ORIGIN      
        1 mderrvadyf viaglpgqdn dfasdnende kledwyqegt hvkdvhmkpp itdlaiifpa
       61 lgeqcpegyt vlehtvsglp adlnhgslrt necylcyrrg rdkpplvdig viydgkerim
      121 qdaemvletp kghlanvnns sskifvtyrr ashkmacnsl vvtdicvilt nkgetpphaf
      181 cvinknlnkg mvgsdvylcy kksmnranli sfkpailfky ptsdyssfvf pnsvamfclp
      241 mgatiecwpk vackpkpvfs tfvltvadaa qkiygsaitf yeeleleidi snfkeneplt
      301 dvlmenmkns vehtdidyqe lktrihkqpg ifkkltpaql eklqftnpat qslnisksic
      361 ilshwpffda fekflvflhs mcnnklqnvp iekyiayfly dipfpspqrp rifvqlsand
      421 rlvltqpedl alprsgasfr qllinlgpdn cllilllilt eqkilvhslr pdvltsvsea
      481 iamilfpfkw qcpyiplcpl glaevlhapl pfligvdsrf fdlydppsdv ncvnldtnni
      541 aicddkkyln vkllpkkaar qlrncldhly selislgkry ssmkdtddee fsvdkdfqlk
      601 rkeqaielei qdaflrftat vlkgyrafll pitkaptvgs tdptslfniq aflrsrdkah
      661 akfysmlvkt qmfirfieet sfvsdmdmag laffdecter ieeenmplle ldesqhsert
      721 vyipppeagp nqlpityrtf klnpelirpl knfniknpnl safgiipgsp marrtkheik
      781 vaqrmarkqa ampdrwgrcl lgtcyslwfl hlpamlqvsn qpatilhqay dllvkmpklh
      841 ldpieevcyr ammqlcgvyg qpvlavkllf hmkrsgvqpn altygfynka vleatwpsdm
      901 tnssqlmwnk lrnaivgaal fkragkrsar rrlssnaell avdgktasme hafsrsslds
      961 shsqdteaah sdstkgssvs dlpdygpfei kklprqnsiv kdsalnviqt eeldniqlnq
     1021 rmirnmespl kspvrtpvte ndplgalmnd etpvvspsee ndsncstctl tifnnveerp
     1081 ggpllfrssi lprsatfhqt vdeagvaigg qlqrsetmph svaqqgeqdk ererleissi
     1141 wsqkdsvtss lsslgsslkl sfgstsitgk ksneiilggl sslksaattv vkkfdeikea
     1201 isatstpvkl kereykimhg ashesldsmt dgsiqdrnep lrmsgdgnld sytlydlaes
     1261 lypkgsreld eriaielvls sasrchqcas ilydeeimag wqpedsnlnt vcqycdkatv
     1321 plltitildy rceenekknd plmgalvell dkpkellepi tvpylnplvl rkelesvlsq
     1381 egdtcltkhk fidehpivyw nliwyferin lsshlpdlwl gndkkkeqin ynavgvktmw
     1441 dnerlhmdrl pmylqwklnn vdkslmqsvi tyvrrndlae pikrvalers knpadqslfs
     1501 iyrdilflaf tvlgrgnidq gvfdkeysls lerltekeek llckidapps tmsvfcrhyf
     1561 kqlsv
//
LOCUS       XP_034194847            1565 aa            linear   INV 03-SEP-2025
DEFINITION  DENN domain-containing protein Crag isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194847
VERSION     XP_034194847.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338956.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1565
                     /product="DENN domain-containing protein Crag isoform X1"
                     /calculated_mol_wt=177007
     Region          50..>214
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     Region          198..281
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          355..538
                     /region_name="DENN"
                     /note="DENN (AEX-3) domain; pfam02141"
                     /db_xref="CDD:460461"
     Region          607..680
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          846..875
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          847..884
                     /region_name="PPR_2"
                     /note="PPR repeat family; pfam13041"
                     /db_xref="CDD:463778"
     CDS             1..1565
                     /gene="Crag"
                     /coded_by="XM_034338956.2:336..5033"
                     /db_xref="GeneID:117611071"
ORIGIN      
        1 mderrvadyf viaglpgqdn dfasdnende kledwyqegt hvkdvhmkpp itdlaiifpa
       61 lgeqcpegyt vlehtvsglp adlnhgslrt necylcyrrg rdkpplvdig viydgkerim
      121 qdaemvletp kghlanvnns sskifvtyrr ashkmacnsl vvtdicvilt nkgetpphaf
      181 cvinknlnkg mvgsdvylcy kksmnranli sfkpailfky ptsdyssfvf pnsvamfclp
      241 mgatiecwpk vackpkpvfs tfvltvadaa qkiygsaitf yeeleleidi snfkeneplt
      301 dvlmenmkns vehtdidyqe lktrihkqpg ifkkltpaql eklqftnpat qslnisksic
      361 ilshwpffda fekflvflhs mcnnklqnvp iekyiayfly dipfpspqrp rifvqlsand
      421 rlvltqpedl alprsgasfr qllinlgpdn cllilllilt eqkilvhslr pdvltsvsea
      481 iamilfpfkw qcpyiplcpl glaevlhapl pfligvdsrf fdlydppsdv ncvnldtnni
      541 aicddkkyln vkllpkkaar qlrncldhly selislgkry ssmkdtddee fsvdkdfqlk
      601 rkeqaielei qdaflrftat vlkgyrafll pitkaptvgs tdptslfniq aflrsrdkah
      661 akfysmlvkt qmfirfieet sfvsdmdmag laffdecter ieeenmplle ldesqhsert
      721 vyipppeagp nqlpityrtf klnpelirpl knfniknpnl safgiipgsp marrtkheik
      781 vaqrmarkqa ampdrwgrcl lgtcyslwfl hlpamlqvsn qpatilhqay dllvkmpklh
      841 ldpieevcyr ammqlcgvyg qpvlavkllf hmkrsgvqpn altygfynka vleatwpsdm
      901 tnssqlmwnk lrnaivgaal fkragkrsar rrlssnaell avdgktasme hafsrsslds
      961 shsqdteaah sdstkgssvs dlpdygpfei kklprqnsiv kdsalnviqt eeldniqlnq
     1021 rmirnmespl kspvrtpvte ndplgalmnd etpvvspsee ndsncstctl tifnnveerp
     1081 ggpllfrssi lprsatfhqt vdeagvaigg qlqrsetmph svaqqgeqdk ererleissi
     1141 wsqkdsvtss lsslgsslkl sfgstsitgk ksneiilggl sslksaattv vkkfdeikea
     1201 isatstpvkl kereykimhg ashesldsmt dgsiqdrnep lrmsgdgnld sytlydlaes
     1261 lypkgsreld eriaielvls sasrchqcas ilydeeimag wqpedsnlnt vcqycdkatv
     1321 plltitildy rceenekknd plmgalvell dkpkellepi tvpylnplvl rkelesvlsq
     1381 egdtcltkhk fidehpivyw nliwyferin lsshlpdlwl gndkkkeqin ynavgvktmw
     1441 dnerlhmdrl pmylqwklnn vdkslmqsvi tyvrrndlae pikrvalers knpadqslfs
     1501 iyrdilflaf tvlgrgnidq gvfdkeysls lerltekeek llckidapps tmsvfcrhyf
     1561 kqlsv
//
LOCUS       XP_034194851            1565 aa            linear   INV 03-SEP-2025
DEFINITION  DENN domain-containing protein Crag isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194851
VERSION     XP_034194851.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338960.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1565
                     /product="DENN domain-containing protein Crag isoform X1"
                     /calculated_mol_wt=177007
     Region          50..>214
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     Region          198..281
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          355..538
                     /region_name="DENN"
                     /note="DENN (AEX-3) domain; pfam02141"
                     /db_xref="CDD:460461"
     Region          607..680
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          846..875
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          847..884
                     /region_name="PPR_2"
                     /note="PPR repeat family; pfam13041"
                     /db_xref="CDD:463778"
     CDS             1..1565
                     /gene="Crag"
                     /coded_by="XM_034338960.2:469..5166"
                     /db_xref="GeneID:117611071"
ORIGIN      
        1 mderrvadyf viaglpgqdn dfasdnende kledwyqegt hvkdvhmkpp itdlaiifpa
       61 lgeqcpegyt vlehtvsglp adlnhgslrt necylcyrrg rdkpplvdig viydgkerim
      121 qdaemvletp kghlanvnns sskifvtyrr ashkmacnsl vvtdicvilt nkgetpphaf
      181 cvinknlnkg mvgsdvylcy kksmnranli sfkpailfky ptsdyssfvf pnsvamfclp
      241 mgatiecwpk vackpkpvfs tfvltvadaa qkiygsaitf yeeleleidi snfkeneplt
      301 dvlmenmkns vehtdidyqe lktrihkqpg ifkkltpaql eklqftnpat qslnisksic
      361 ilshwpffda fekflvflhs mcnnklqnvp iekyiayfly dipfpspqrp rifvqlsand
      421 rlvltqpedl alprsgasfr qllinlgpdn cllilllilt eqkilvhslr pdvltsvsea
      481 iamilfpfkw qcpyiplcpl glaevlhapl pfligvdsrf fdlydppsdv ncvnldtnni
      541 aicddkkyln vkllpkkaar qlrncldhly selislgkry ssmkdtddee fsvdkdfqlk
      601 rkeqaielei qdaflrftat vlkgyrafll pitkaptvgs tdptslfniq aflrsrdkah
      661 akfysmlvkt qmfirfieet sfvsdmdmag laffdecter ieeenmplle ldesqhsert
      721 vyipppeagp nqlpityrtf klnpelirpl knfniknpnl safgiipgsp marrtkheik
      781 vaqrmarkqa ampdrwgrcl lgtcyslwfl hlpamlqvsn qpatilhqay dllvkmpklh
      841 ldpieevcyr ammqlcgvyg qpvlavkllf hmkrsgvqpn altygfynka vleatwpsdm
      901 tnssqlmwnk lrnaivgaal fkragkrsar rrlssnaell avdgktasme hafsrsslds
      961 shsqdteaah sdstkgssvs dlpdygpfei kklprqnsiv kdsalnviqt eeldniqlnq
     1021 rmirnmespl kspvrtpvte ndplgalmnd etpvvspsee ndsncstctl tifnnveerp
     1081 ggpllfrssi lprsatfhqt vdeagvaigg qlqrsetmph svaqqgeqdk ererleissi
     1141 wsqkdsvtss lsslgsslkl sfgstsitgk ksneiilggl sslksaattv vkkfdeikea
     1201 isatstpvkl kereykimhg ashesldsmt dgsiqdrnep lrmsgdgnld sytlydlaes
     1261 lypkgsreld eriaielvls sasrchqcas ilydeeimag wqpedsnlnt vcqycdkatv
     1321 plltitildy rceenekknd plmgalvell dkpkellepi tvpylnplvl rkelesvlsq
     1381 egdtcltkhk fidehpivyw nliwyferin lsshlpdlwl gndkkkeqin ynavgvktmw
     1441 dnerlhmdrl pmylqwklnn vdkslmqsvi tyvrrndlae pikrvalers knpadqslfs
     1501 iyrdilflaf tvlgrgnidq gvfdkeysls lerltekeek llckidapps tmsvfcrhyf
     1561 kqlsv
//
LOCUS       XP_076546565            1557 aa            linear   INV 03-SEP-2025
DEFINITION  DENN domain-containing protein Crag isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546565
VERSION     XP_076546565.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690450.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1557
                     /product="DENN domain-containing protein Crag isoform X2"
                     /calculated_mol_wt=176321
     Region          50..>214
                     /region_name="DUF2464"
                     /note="Multivesicular body subunit 12; pfam10240"
                     /db_xref="CDD:463017"
     Region          198..281
                     /region_name="uDENN"
                     /note="Domain always found upstream of DENN domain, found
                     in a variety of signalling proteins; smart00800"
                     /db_xref="CDD:214824"
     Region          355..538
                     /region_name="DENN"
                     /note="DENN (AEX-3) domain; pfam02141"
                     /db_xref="CDD:460461"
     Region          607..680
                     /region_name="dDENN"
                     /note="Domain always found downstream of DENN domain,
                     found in a variety of signalling proteins; smart00801"
                     /db_xref="CDD:129037"
     Region          846..875
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          847..884
                     /region_name="PPR_2"
                     /note="PPR repeat family; pfam13041"
                     /db_xref="CDD:463778"
     CDS             1..1557
                     /gene="Crag"
                     /coded_by="XM_076690450.1:428..5101"
                     /db_xref="GeneID:117611071"
ORIGIN      
        1 mderrvadyf viaglpgqdn dfasdnende kledwyqegt hvkdvhmkpp itdlaiifpa
       61 lgeqcpegyt vlehtvsglp adlnhgslrt necylcyrrg rdkpplvdig viydgkerim
      121 qdaemvletp kghlanvnns sskifvtyrr ashkmacnsl vvtdicvilt nkgetpphaf
      181 cvinknlnkg mvgsdvylcy kksmnranli sfkpailfky ptsdyssfvf pnsvamfclp
      241 mgatiecwpk vackpkpvfs tfvltvadaa qkiygsaitf yeeleleidi snfkeneplt
      301 dvlmenmkns vehtdidyqe lktrihkqpg ifkkltpaql eklqftnpat qslnisksic
      361 ilshwpffda fekflvflhs mcnnklqnvp iekyiayfly dipfpspqrp rifvqlsand
      421 rlvltqpedl alprsgasfr qllinlgpdn cllilllilt eqkilvhslr pdvltsvsea
      481 iamilfpfkw qcpyiplcpl glaevlhapl pfligvdsrf fdlydppsdv ncvnldtnni
      541 aicddkkyln vkllpkkaar qlrncldhly selislgkry ssmkdtddee fsvdkdfqlk
      601 rkeqaielei qdaflrftat vlkgyrafll pitkaptvgs tdptslfniq aflrsrdkah
      661 akfysmlvkt qmfirfieet sfvsdmdmag laffdecter ieeenmplle ldesqhsert
      721 vyipppeagp nqlpityrtf klnpelirpl knfniknpnl safgiipgsp marrtkheik
      781 vaqrmarkqa ampdrwgrcl lgtcyslwfl hlpamlqvsn qpatilhqay dllvkmpklh
      841 ldpieevcyr ammqlcgvyg qpvlavkllf hmkrsgvqpn altygfynka vleatwpsdm
      901 tnssqlmwnk lrnaivgaal fkragkrsar rrlssnaell avdgktasme hafsrsslds
      961 shsqdteaah sdylpdygpf eikklprqns ivkdsalnvi qteeldniql nqrmirnmes
     1021 plkspvrtpv tendplgalm ndetpvvsps eendsncstc tltifnnvee rpggpllfrs
     1081 silprsatfh qtvdeagvai ggqlqrsetm phsvaqqgeq dkererleis siwsqkdsvt
     1141 sslsslgssl klsfgstsit gkksneiilg glsslksaat tvvkkfdeik eaisatstpv
     1201 klkereykim hgasheslds mtdgsiqdrn eplrmsgdgn ldsytlydla eslypkgsre
     1261 lderiaielv lssasrchqc asilydeeim agwqpedsnl ntvcqycdka tvplltitil
     1321 dyrceenekk ndplmgalve lldkpkelle pitvpylnpl vlrkelesvl sqegdtcltk
     1381 hkfidehpiv ywnliwyfer inlsshlpdl wlgndkkkeq inynavgvkt mwdnerlhmd
     1441 rlpmylqwkl nnvdkslmqs vityvrrndl aepikrvale rsknpadqsl fsiyrdilfl
     1501 aftvlgrgni dqgvfdkeys lslerlteke ekllckidap pstmsvfcrh yfkqlsv
//
LOCUS       XP_034191727             595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609461 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191727
VERSION     XP_034191727.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335836.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191727.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..595
                     /product="uncharacterized protein LOC117609461 isoform X1"
                     /calculated_mol_wt=65334
     Region          <300..470
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..595
                     /gene="LOC117609461"
                     /coded_by="XM_034335836.2:10..1797"
                     /db_xref="GeneID:117609461"
ORIGIN      
        1 mgssviellt kmkvsvilll vsvswvvafp gpqwnveekg kdgssrghes liglggligg
       61 dgkdggllsg lvsgkgkinp lellvkkgdg ggkgkedknl ewssiskvii sqggdentvn
      121 klkgilakge gswgvnekki lqqvsvllkg egkpdlmeti kkeveklgkg ggkghgwseg
      181 riehaqsdvk vkkesvgavk eshgkksehg gikitehsge dheekgrkgy kssssseshs
      241 ksskdsssgk gsshshgkss ssdshskssk dsssdshsks ssssssesse ewhnhhgkrr
      301 skkveirghv ehknighgdh kkevlvehkn eghvehknee hvehkvegha ehkveghdhk
      361 revhvehkpe ihdehkvveq vehkveghde hkaeehgkev qvelkkdghd dhkteghdeh
      421 kvveqvehkv eghdehkaee hgkevqvdlk kdehddlkgk gdvehknveq vghgkeeevv
      481 ievqvkeqit kiaeekvlae lkksgkgkek lteeekkics etimkkveeq vvivkkggkk
      541 ledaeilkig eeviekelke gnllkllets qktevvdekk evvskgkddg mekki
//
LOCUS       XP_034191737             594 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609461 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191737
VERSION     XP_034191737.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335846.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191737.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..594
                     /product="uncharacterized protein LOC117609461 isoform X2"
                     /calculated_mol_wt=65277
     Region          <299..469
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..594
                     /gene="LOC117609461"
                     /coded_by="XM_034335846.2:10..1794"
                     /db_xref="GeneID:117609461"
ORIGIN      
        1 mgssviellt kmkvsvilll vsvswvvafp gpqwnveekg kdgssrghes liglggligg
       61 dgkdggllsg lvsgkgkinp lellvkkgdg gkgkedknle wssiskviis qggdentvnk
      121 lkgilakgeg swgvnekkil qqvsvllkge gkpdlmetik keveklgkgg gkghgwsegr
      181 iehaqsdvkv kkesvgavke shgkksehgg ikitehsged heekgrkgyk ssssseshsk
      241 sskdsssgkg sshshgksss sdshsksskd sssdshskss sssssessee whnhhgkrrs
      301 kkveirghve hknighgdhk kevlvehkne ghvehkneeh vehkveghae hkveghdhkr
      361 evhvehkpei hdehkvveqv ehkveghdeh kaeehgkevq velkkdghdd hkteghdehk
      421 vveqvehkve ghdehkaeeh gkevqvdlkk dehddlkgkg dvehknveqv ghgkeeevvi
      481 evqvkeqitk iaeekvlael kksgkgkekl teeekkicse timkkveeqv vivkkggkkl
      541 edaeilkige eviekelkeg nllklletsq ktevvdekke vvskgkddgm ekki
//
LOCUS       XP_034191655             313 aa            linear   INV 03-SEP-2025
DEFINITION  G-protein coupled receptor DopEcR [Osmia lignaria lignaria].
ACCESSION   XP_034191655
VERSION     XP_034191655.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335764.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..313
                     /product="G-protein coupled receptor DopEcR"
                     /calculated_mol_wt=35467
     Region          11..281
                     /region_name="7tm_classA_rhodopsin-like"
                     /note="rhodopsin receptor-like class A family of the
                     seven-transmembrane G protein-coupled receptor
                     superfamily; cd00637"
                     /db_xref="CDD:410626"
     Region          11..36
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410626"
     Region          43..69
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410626"
     Site            order(65,68..69,81..86,88..89,92,137,139..143,166,
                     169..171,173..175,177..178,237,240..241,243..244,247,
                     255..256,258..260,263,266..267)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410626"
     Region          81..111
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410626"
     Region          124..143
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410626"
     Region          166..191
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410626"
     Region          217..247
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410626"
     Region          256..281
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410626"
     CDS             1..313
                     /gene="DopEcR"
                     /coded_by="XM_034335764.2:695..1636"
                     /db_xref="GeneID:117609433"
ORIGIN      
        1 meepslealm qaglifvvgv aiilsnllii atylnfrgps evinyyllsl asadllcgll
       61 vvplsvypal vrrwvygdiv crlvgylevt lwavsvytfm wisvdrylai rkplryetvq
      121 tktrcqcwmv ftwisvammc cppllgfqkp ifdreaficm ldwgnmaayt itlsilvlgp
      181 svitiiytys yiftmmrrlr sgvlihdkey atalsenlsn pshimsfvlv mtfwmswapy
      241 aglriyavvn gppqvpflhf avvwlgitns fwkavvlgtl spqfrlaarv lcltlccrhr
      301 rlppellgld ddd
//
LOCUS       XP_034195958             511 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome P450 9e2-like [Osmia lignaria lignaria].
ACCESSION   XP_034195958
VERSION     XP_034195958.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340067.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195958.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="cytochrome P450 9e2-like"
                     /calculated_mol_wt=59031
     Region          70..491
                     /region_name="CYP6-like"
                     /note="cytochrome P450 family 6 and similar cytochrome
                     P450s; cd11056"
                     /db_xref="CDD:410679"
     Site            order(128,132,139,186,295..296,299..300,303..304,307,359,
                     368,370,400,434..436,440..444,447..448)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410679"
     Site            order(212..213,295,298..299,303,366..369)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:410679"
     CDS             1..511
                     /gene="LOC117611784"
                     /coded_by="XM_034340067.2:257..1792"
                     /db_xref="GeneID:117611784"
ORIGIN      
        1 mdpftltlfl tmgflllyhf lwqpmnyfkq rgikyespvp ifgnmasvvl rrttmaehfq
       61 rlyhrfsdvk yfglfnftsp vivirdpdli asitvknfdh fsdrrgfdvg dldpligrnl
      121 salrgdewre mrrllspsft smkmkimyhl mrdcandftd fianqskhgk vfdikdifgr
      181 yttdviatcs fgisidsmrn pnnefyvlar dtmssqssms wklmlamccp alcrtfgirv
      241 fsekvhryff nvvrqtvkmr eeqgitrpdm iqlmmdtkdk ersltieemt nqafvfflag
      301 ydtsstflsf liheiathpe vkaklmeeie evvrktngnp tydalknmpy mdavmnetlr
      361 ldgiaisldr vcvkefqlep assgaepvtl kpgdivwflp fslqrdskyv knptkfdpdr
      421 flgkdapsps vnipfgigpr icignrfall eskilmfyll wrcdvepcek tqvpmkfskh
      481 nialtaengf wlkfrardkv clqeangkvk n
//
LOCUS       XP_034195964             470 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611787 [Osmia lignaria lignaria].
ACCESSION   XP_034195964
VERSION     XP_034195964.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340073.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195964.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="uncharacterized protein LOC117611787"
                     /calculated_mol_wt=53228
     Region          71..446
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          72..98
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          105..130
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(127,130..131,143..148,150..151,154,199,201..205,238,
                     241..243,390,393..394,396..397,400,413..414,418..420,423,
                     426..427)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          143..173
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          185..205
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          283..401
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          414..441
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..470
                     /gene="LOC117611787"
                     /coded_by="XM_034340073.2:2721..4133"
                     /db_xref="GeneID:117611787"
ORIGIN      
        1 mfvttstlnp ntrcieaaat measrarrsv sptmwvnlth yayrnrsfea gidfeekpid
       61 achplyflld ffhqyyipsi illglvgnll scvvflnthl rirsssyyla alatadfgfl
      121 vtlllvwlnn tlgwkvfnkd gwcetlvyvs avcsslsvwl ivaftverfi avqyplhrph
      181 ictiarakti vlvlvilalt shsysfvtag vvssrdgeey cdlkveylet mkiisiidsi
      241 aslivplvli ivmntmimrn llrfsrrfkq tsasstdsps rersdinlnq ipsgsssnhg
      301 ggginlgpmv ggrrppsqqs fhssknnhsh hsrhqttsap sstprnacql peiaarcihi
      361 rsssrnlvst rnqqsitkml llistvfill nlpsyvirlc vffftlarkn tpdllwclqq
      421 ffmllyytnf sinfllyamc gitfrrclqq llrkllksmt ryhcnpqryi
//
LOCUS       XP_034195965             470 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611787 [Osmia lignaria lignaria].
ACCESSION   XP_034195965
VERSION     XP_034195965.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340074.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195965.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="uncharacterized protein LOC117611787"
                     /calculated_mol_wt=53228
     Region          71..446
                     /region_name="7tmA_FMRFamide_R-like"
                     /note="FMRFamide (Phe-Met-Arg-Phe) receptors and related
                     proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd14978"
                     /db_xref="CDD:410630"
     Region          72..98
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410630"
     Region          105..130
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410630"
     Site            order(127,130..131,143..148,150..151,154,199,201..205,238,
                     241..243,390,393..394,396..397,400,413..414,418..420,423,
                     426..427)
                     /site_type="other"
                     /note="putative ligand binding pocket [chemical binding]"
                     /db_xref="CDD:410630"
     Region          143..173
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410630"
     Region          185..205
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410630"
     Region          283..401
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410630"
     Region          414..441
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410630"
     CDS             1..470
                     /gene="LOC117611787"
                     /coded_by="XM_034340074.2:2708..4120"
                     /db_xref="GeneID:117611787"
ORIGIN      
        1 mfvttstlnp ntrcieaaat measrarrsv sptmwvnlth yayrnrsfea gidfeekpid
       61 achplyflld ffhqyyipsi illglvgnll scvvflnthl rirsssyyla alatadfgfl
      121 vtlllvwlnn tlgwkvfnkd gwcetlvyvs avcsslsvwl ivaftverfi avqyplhrph
      181 ictiarakti vlvlvilalt shsysfvtag vvssrdgeey cdlkveylet mkiisiidsi
      241 aslivplvli ivmntmimrn llrfsrrfkq tsasstdsps rersdinlnq ipsgsssnhg
      301 ggginlgpmv ggrrppsqqs fhssknnhsh hsrhqttsap sstprnacql peiaarcihi
      361 rsssrnlvst rnqqsitkml llistvfill nlpsyvirlc vffftlarkn tpdllwclqq
      421 ffmllyytnf sinfllyamc gitfrrclqq llrkllksmt ryhcnpqryi
//
LOCUS       XP_034195150             564 aa            linear   INV 03-SEP-2025
DEFINITION  modular serine protease isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195150
VERSION     XP_034195150.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339259.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195150.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="modular serine protease isoform X1"
                     /calculated_mol_wt=62221
     Region          116..150
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(121,129,140..141)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(133,136,140,146..147)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            143..147
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          160..215
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          309..558
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            309
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(353,411,511)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(505,530,536)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..564
                     /gene="LOC117611313"
                     /coded_by="XM_034339259.2:96..1790"
                     /db_xref="GeneID:117611313"
ORIGIN      
        1 mntlvaltla fslltlsasl adhgvkrspk ppwarpvnnn yneqtnyneh rgppggippg
       61 lsgrvppgls gdappgksqg kgqgkpggkp swsgelidek eimnssqqlg dnevyckkne
      121 frcrngeclp stmrcdnkfd crdssdeldc vreaenetgc alpeqpeggs yelggcgkpc
      181 pkrsgdkvpr nsiltytcrp nyilngsavs vcvndawynl ssclrtcpsl nsntvnilct
      241 yqgnevscsd rirpgtvatl sckssyklpv tndpgyrtvt cledglwdrr lfrclpecgt
      301 pvshgntlvv ngfeaklgif pwhvgiyskd kdeyeqicgg tlisnnliis aahcfydevy
      361 nkvndksqya iaagkhyrnw daneqyaqks mvkeillper yigaranfaq dialiklitp
      421 feltalvrpi cidwdseyer vqlqvgqsgk svgwgktikd tssetlqevn mpfvpydqcl
      481 savpydfrgy itsdkfcagr lngsslcegd sggglcfkih etwyirgvvs vspvkadrcd
      541 yhsytvftst dyfrdwiraa yins
//
LOCUS       XP_034195152             549 aa            linear   INV 03-SEP-2025
DEFINITION  modular serine protease isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195152
VERSION     XP_034195152.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339261.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195152.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..549
                     /product="modular serine protease isoform X2"
                     /calculated_mol_wt=60615
     Region          101..135
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(106,114,125..126)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(118,121,125,131..132)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            128..132
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     Region          145..200
                     /region_name="CCP"
                     /note="Domain abundant in complement control proteins;
                     SUSHI repeat; short complement-like repeat (SCR);
                     smart00032"
                     /db_xref="CDD:214478"
     Region          294..543
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            294
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(338,396,496)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(490,515,521)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..549
                     /gene="LOC117611313"
                     /coded_by="XM_034339261.2:108..1757"
                     /db_xref="GeneID:117611313"
ORIGIN      
        1 mgapvadhgv krspkppwar pvnnnyneqt nynehrgppg gippglsgrv ppglsgdapp
       61 gksqgkgqgk pggkpswsge lidekeimns sqqlgdnevy ckknefrcrn geclpstmrc
      121 dnkfdcrdss deldcvreae netgcalpeq peggsyelgg cgkpcpkrsg dkvprnsilt
      181 ytcrpnyiln gsavsvcvnd awynlssclr tcpslnsntv nilctyqgne vscsdrirpg
      241 tvatlsckss yklpvtndpg yrtvtcledg lwdrrlfrcl pecgtpvshg ntlvvngfea
      301 klgifpwhvg iyskdkdeye qicggtlisn nliisaahcf ydevynkvnd ksqyaiaagk
      361 hyrnwdaneq yaqksmvkei llperyigar anfaqdiali klitpfelta lvrpicidwd
      421 seyervqlqv gqsgksvgwg ktikdtsset lqevnmpfvp ydqclsavpy dfrgyitsdk
      481 fcagrlngss lcegdsgggl cfkihetwyi rgvvsvspvk adrcdyhsyt vftstdyfrd
      541 wiraayins
//
LOCUS       XP_034195154             526 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611322 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195154
VERSION     XP_034195154.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339263.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195154.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..526
                     /product="uncharacterized protein LOC117611322 isoform X1"
                     /calculated_mol_wt=59812
     Region          14..51
                     /region_name="DD_CABYR_SP17"
                     /note="dimerization/docking (D/D) domain of the sperm
                     fibrous sheath proteins, Calcium-Binding
                     tYrosine-phosphorylation Regulated protein and Sperm
                     Protein 17; cd12100"
                     /db_xref="CDD:438521"
     Site            order(16,20..21,24..25,28..29,32..33)
                     /site_type="other"
                     /note="putative AKAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:438521"
     Site            order(20,23..24,27..28,31..32,35..36,38..41,43..44,47..48,
                     51)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:438521"
     Region          <93..>162
                     /region_name="Adgb_C_mid-like"
                     /note="C-terminal middle region of Androglobins (Adgbs)
                     and related proteins; including permuted globin domain and
                     IQ motif; cd22307"
                     /db_xref="CDD:412094"
     Region          98..125
                     /region_name="globin helix H"
                     /note="globin helix H [structural motif]"
                     /db_xref="CDD:412094"
     Region          146..162
                     /region_name="IQ motif"
                     /note="IQ motif [structural motif]"
                     /db_xref="CDD:412094"
     Region          <378..>526
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..526
                     /gene="LOC117611322"
                     /coded_by="XM_034339263.2:554..2134"
                     /db_xref="GeneID:117611322"
ORIGIN      
        1 mnvtlqkhdg khiykvpegl relctdisre vlrsqpanly hfiseyvdtl litrentkia
       61 vkvvnnillg seailsilyr agftleqiav aapriqrafr eyldavdmhp vqicddyshd
      121 ekslmsiqsi leatgatred aeraatviqa afrghyerma lneaqgkvqw qravtntldi
      181 lkkagasqte iskavehlkf ayrgyytrrn qrldalgieq eplkkderil epgeaiqava
      241 wmemmyedsg ltvekaneaa cviqraykky rarrrcydvq klesskslvv eaildsvrhm
      301 vfervtsrqd ipteygtqee lmiastkvql tikqhlrktr fsresylqst eeeyegeqly
      361 deeeeefede ergegavaep qskeleakag adyveeqtee kddakeqape aevtehvhps
      421 pgleqpeeae lkevekeaee vhelketedq ekvaevteva qetevakete maeekevaek
      481 seveketkvp eetektevaq qtekaeeaee pnvaqetkgt sdagae
//
LOCUS       XP_076546566             521 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611322 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546566
VERSION     XP_076546566.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690451.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..521
                     /product="uncharacterized protein LOC117611322 isoform X2"
                     /calculated_mol_wt=59205
     Region          14..51
                     /region_name="DD_CABYR_SP17"
                     /note="dimerization/docking (D/D) domain of the sperm
                     fibrous sheath proteins, Calcium-Binding
                     tYrosine-phosphorylation Regulated protein and Sperm
                     Protein 17; cd12100"
                     /db_xref="CDD:438521"
     Site            order(16,20..21,24..25,28..29,32..33)
                     /site_type="other"
                     /note="putative AKAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:438521"
     Site            order(20,23..24,27..28,31..32,35..36,38..41,43..44,47..48,
                     51)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:438521"
     Region          <93..>162
                     /region_name="Adgb_C_mid-like"
                     /note="C-terminal middle region of Androglobins (Adgbs)
                     and related proteins; including permuted globin domain and
                     IQ motif; cd22307"
                     /db_xref="CDD:412094"
     Region          98..125
                     /region_name="globin helix H"
                     /note="globin helix H [structural motif]"
                     /db_xref="CDD:412094"
     Region          146..162
                     /region_name="IQ motif"
                     /note="IQ motif [structural motif]"
                     /db_xref="CDD:412094"
     Region          <376..520
                     /region_name="rne"
                     /note="ribonuclease E; Reviewed; PRK10811"
                     /db_xref="CDD:236766"
     CDS             1..521
                     /gene="LOC117611322"
                     /coded_by="XM_076690451.1:554..2119"
                     /db_xref="GeneID:117611322"
ORIGIN      
        1 mnvtlqkhdg khiykvpegl relctdisre vlrsqpanly hfiseyvdtl litrentkia
       61 vkvvnnillg seailsilyr agftleqiav aapriqrafr eyldavdmhp vqicddyshd
      121 ekslmsiqsi leatgatred aeraatviqa afrghyerma lneaqgkvqw qravtntldi
      181 lkkagasqte iskavehlkf ayrgyytrrn qrldalgieq eplkkderil epgeaiqava
      241 wmemmyedsg ltvekaneaa cviqraykky rarrrcydvq klesskslvv eaildsvrhm
      301 vfervtsrqd ipteygtqee lmiastkvql tikqhlrktr fsresylqst eeeyegeqly
      361 deeeeefede ergegavaep qskeleakag adyveeqtee kddakeqape aevtehvhps
      421 pgleqpeeae lkevekeaee etedqekvae vtevaqetev aketemaeek evaeksevek
      481 etkvpeetek tevaqqteka eeaeepnvaq etkgtsdaga e
//
LOCUS       XP_034195158             483 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611327 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195158
VERSION     XP_034195158.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339267.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195158.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="uncharacterized protein LOC117611327 isoform X1"
                     /calculated_mol_wt=54697
     CDS             1..483
                     /gene="LOC117611327"
                     /coded_by="XM_034339267.2:136..1587"
                     /db_xref="GeneID:117611327"
ORIGIN      
        1 mseicpsssh qlvaaaiafr rarrkfpkny glccnpeemt kfdetesiae neepndpenl
       61 sftsrkclng ksncaeaiwt decgesvsik enlvqtpiic sccrkrneqc ssgdaldivp
      121 skehngiark necflpeqse nndgkamkgf iteprnstrc rtsyshsrrr spketsskld
      181 svnllydrgh deadnvkgsn ssekrncnre eyscsggrkd sksvhrnsrs klnvcdsrkg
      241 npsdnvshnc dfhcvkhsip dslkshtdcc rlqnhrpccy sqnhchhcce khsystkdka
      301 rltsdtclcs snldnhtdcc nqdhvtygsi cnhdccikql gkmsgmqeqk deelddcvgt
      361 ihhkdslcil vekykanrkc kikkysdgse icderardec dksftsettc qldeinakqr
      421 ekcldnnehr frenrtligk tcrhgcgqif regdhshlkn lksrliktgt ccspkgtpwr
      481 htf
//
LOCUS       XP_034195163             475 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611327 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195163
VERSION     XP_034195163.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339272.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195163.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="uncharacterized protein LOC117611327 isoform X2"
                     /calculated_mol_wt=53852
     CDS             1..475
                     /gene="LOC117611327"
                     /coded_by="XM_034339272.2:136..1563"
                     /db_xref="GeneID:117611327"
ORIGIN      
        1 mseicpsssh qlvaaaiafr rarrkfpkny glccnpeemt kfdetesiae neepndpenl
       61 sftsrkclng ksncaeaiwt decgesvsik enlvqtpiic sccrkrneqc ssgdaldivp
      121 skehngiark necflpeqse nndgkamkgf iteprnstrc rtsyshsrrr spketsskld
      181 svnllydrgh deadnvkdcn reeyscsggr kdsksvhrns rsklnvcdsr kgnpsdnvsh
      241 ncdfhcvkhs ipdslkshtd ccrlqnhrpc cysqnhchhc cekhsystkd karltsdtcl
      301 cssnldnhtd ccnqdhvtyg sicnhdccik qlgkmsgmqe qkdeelddcv gtihhkdslc
      361 ilvekykanr kckikkysdg seicderard ecdksftset tcqldeinak qrekcldnne
      421 hrfrenrtli gktcrhgcgq ifregdhshl knlksrlikt gtccspkgtp wrhtf
//
LOCUS       XP_076546567             699 aa            linear   INV 03-SEP-2025
DEFINITION  peroxidasin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546567
VERSION     XP_076546567.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690452.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..699
                     /product="peroxidasin isoform X1"
                     /calculated_mol_wt=78388
     Region          140..667
                     /region_name="An_peroxidase"
                     /note="Animal haem peroxidase; pfam03098"
                     /db_xref="CDD:460804"
     CDS             1..699
                     /gene="LOC117611307"
                     /coded_by="XM_076690452.1:370..2469"
                     /db_xref="GeneID:117611307"
ORIGIN      
        1 mkrrpingtf aflsrllalf vlvestilps nmlldlgrrt nismekyhqa eqailtshth
       61 lnhgspswfl gashemteha qnlsrkalri etmavmlvea lnmsskeass vlpsvaaisc
      121 sgsptflqlt nscknvdpry rthtgkcnna lhptwgasme ayvrflpaey vdgvslprid
      181 lpsarevssr vhsggsdlkh pylmaltalf gqflvhdlah tpkmelpdga klkccdvdye
      241 hfhpecfpir adnavgcmey srsaphpgns lqgcklgprq qinqatsyld lspvygssee
      301 vakvlrsgkg gllntqrknl pmpstkyesc rsvnkafpcf lsgdsrvnen pgltlmhvlf
      361 lrehnrvate lgqlnphwdd erlyqearri viaemehity neflpvvlge ttldkyqlrl
      421 tnrgyfrgyd trtdatlsns vasaglffia tltpktldlv dsrsalksge rsllsafyap
      481 qefyeagaid rlivgatagh srkplppgln eilleryfhd gksndiavdy aaqiiqqgrd
      541 hglpsyvrwr sfcnlspinn fddlrglmak dtinrlrrvy rntedidlvt galseaplpn
      601 svlgptflcl lgrtfrnlrl gdrywyenan spgsftlsql eeirkikmaq ilcyngerlh
      661 wiqprafllr drflnemtnc tihtsqspnf aawkeqnts
//
LOCUS       XP_076546568             668 aa            linear   INV 03-SEP-2025
DEFINITION  peroxidasin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546568
VERSION     XP_076546568.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690453.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="peroxidasin isoform X2"
                     /calculated_mol_wt=74900
     Region          109..636
                     /region_name="An_peroxidase"
                     /note="Animal haem peroxidase; pfam03098"
                     /db_xref="CDD:460804"
     CDS             1..668
                     /gene="LOC117611307"
                     /coded_by="XM_076690453.1:550..2556"
                     /db_xref="GeneID:117611307"
ORIGIN      
        1 mlldlgrrtn ismekyhqae qailtshthl nhgspswflg ashemtehaq nlsrkalrie
       61 tmavmlveal nmsskeassv lpsvaaiscs gsptflqltn scknvdpryr thtgkcnnal
      121 hptwgasmea yvrflpaeyv dgvslpridl psarevssrv hsggsdlkhp ylmaltalfg
      181 qflvhdlaht pkmelpdgak lkccdvdyeh fhpecfpira dnavgcmeys rsaphpgnsl
      241 qgcklgprqq inqatsyldl spvygsseev akvlrsgkgg llntqrknlp mpstkyescr
      301 svnkafpcfl sgdsrvnenp gltlmhvlfl rehnrvatel gqlnphwdde rlyqearriv
      361 iaemehityn eflpvvlget tldkyqlrlt nrgyfrgydt rtdatlsnsv asaglffiat
      421 ltpktldlvd srsalksger sllsafyapq efyeagaidr livgataghs rkplppglne
      481 illeryfhdg ksndiavdya aqiiqqgrdh glpsyvrwrs fcnlspinnf ddlrglmakd
      541 tinrlrrvyr ntedidlvtg alseaplpns vlgptflcll grtfrnlrlg drywyenans
      601 pgsftlsqle eirkikmaqi lcyngerlhw iqprafllrd rflnemtnct ihtsqspnfa
      661 awkeqnts
//
LOCUS       XP_076546569             634 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA processing factor 3 [Osmia lignaria lignaria].
ACCESSION   XP_076546569
VERSION     XP_076546569.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690454.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..634
                     /product="pre-mRNA processing factor 3"
                     /calculated_mol_wt=72323
     Region          4..>56
                     /region_name="PWI"
                     /note="domain in splicing factors; smart00311"
                     /db_xref="CDD:214609"
     Region          101..>188
                     /region_name="Phage-scaffold"
                     /note="Bacteriophage, scaffolding protein; pfam09306"
                     /db_xref="CDD:286399"
     Region          266..476
                     /region_name="PRP3"
                     /note="pre-mRNA processing factor 3 (PRP3); pfam08572"
                     /db_xref="CDD:462524"
     Region          499..620
                     /region_name="DUF1115"
                     /note="Protein of unknown function (DUF1115); pfam06544"
                     /db_xref="CDD:461943"
     CDS             1..634
                     /gene="Prp3"
                     /coded_by="XM_076690454.1:301..2205"
                     /db_xref="GeneID:143305725"
ORIGIN      
        1 msyltrkeid emkpqiekav hkflgfgepa ivttavncit sgydkrktad klsalledkk
       61 askltekifa iyddaraaqk skkrnhvedk dkdkdakkpr fkddevknek tteaqlsadk
      121 irqmmanaqr eieerkralk aikqeesapv kpllkardsl ptvgsmynqg llsktdsdka
      181 rkiaalqaqi rsklssgllg nvnvpdkptp lildesgrtv ditgkevqlt qvvptlkani
      241 rakkreefka qlqeskgpee iqdthffdtr igvkpairgk rtlkfhepgk fqqlaerirm
      301 kaqleklqne isqiarktgi ssatklalia pktealsedv pnvewwdsvi ltggysnede
      361 pttiknstit nlvehptqmr pptdplkpiy mpvfltkker kklrrqnrre awkeeqekir
      421 lglepppepk lrisnlmrvl gteavqdptk ieahvrqqma krlkahedan aarrltadqr
      481 rekkarklke dttlgvhvav yrirdlvnna skkfkvetna kqlyltgcvm lfrdcnvvvv
      541 eggakqlsky krlmmhrikw eediikdndg ndvpnkcvlv wegtskqrhf geikfkvcpi
      601 ekmarehfkk hqvehywdla ysgavldntd dvhs
//
LOCUS       XP_034195369            4092 aa            linear   INV 03-SEP-2025
DEFINITION  dynein cytoplasmic heavy chain beethoven isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195369
VERSION     XP_034195369.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339478.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195369.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4092
                     /product="dynein cytoplasmic heavy chain beethoven isoform
                     X1"
                     /calculated_mol_wt=458121
     Region          <217..609
                     /region_name="DHC_N1"
                     /note="Dynein heavy chain, N-terminal region 1; pfam08385"
                     /db_xref="CDD:462457"
     Region          901..>1297
                     /region_name="YhaN"
                     /note="Uncharacterized conserved protein YhaN, contains
                     AAA domain [Function unknown]; COG4717"
                     /db_xref="CDD:443752"
     Region          1136..1463
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          <1355..3773
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1586..1912
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          <3952..4085
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..4092
                     /gene="btv"
                     /coded_by="XM_034339478.2:74..12352"
                     /db_xref="GeneID:117611538"
ORIGIN      
        1 mvvddrrrsl ivstaghffg vdlkdeqtir deesvldnfl drstcrtlca qpadanfegt
       61 slklsnelpv gkntlvffki eeaavteknf hdlvhvtttg sgkgstlvea lrqvwlptlr
      121 ssgldssylk kleenllgss ttasvleeed ywrkkwedak rshdktiyae tvkilksira
      181 elesaavtrd gligvedave avsgyvddlw klgdavyped rmksfldlig devvslvqnt
      241 ideirtsddr akieaismga eicekwtdma ekfttlfwph hslhpwkgtg yipercsrig
      301 krlrqiaelh aqyrqltrll tanersglgt dnlmqyfddv kvvfdadekg vnwnrlkkri
      361 eeglipaedr vaqklksqia dattpnslla efrryfelmk reglkralrg ereallsayg
      421 dlienclagp dtvnlldspr ilqeiqvars aelrleslnk lgkelladlp gydeissrvt
      481 salkeverkr qnlvdtwteg tkeavaskel tlgtdsavve ltgtsvmrvt ydprlmtlir
      541 earalssqgv efsrevrdli eraellsgra ralqqvatfh ntigdrmvps qrplmlttal
      601 elvravqeqs gvvwsdpqav daytarlkel vrkfaqqnse laakhsllrd lvsnllkgdt
      661 vnlignfntw ketlmnmrtt vdtveaeygn tkawklhwdr qllkalgvay rgalptllkk
      721 lteikveltf rdgslqwrpp leeiraklys airrslsipm nfrgvgdpse ahfsdliqrs
      781 gylfggvykq aeialsalet lrmkwlylaa pakidvaerl kgrspqdwtr afkdakqwaq
      841 evgrlrgnev kiwcisvdta ttrndlesas rrywerlcsd lrveassrlv aiveflssas
      901 kelerrprnv eevgvayeah arieaqssgm aeemeaivgl skvlaawtse klegvnaayt
      961 awqdltdrle rhktvvnrel edakvnlrhr avavrdeier wqakwtskpe ilsldwiism
     1021 rerwtnlkeq ldalktdcqr ivldiddile dneesikkle lqldieesnc rfqaefleel
     1081 knqeeeewtv arrrlprlhd wldswenrir vqlkdqlnee stnqrdslem dtfvgrkvre
     1141 vraaiewlql lrgdeiaeeh wgelmplldl knvknardit lghlfrsvek iqentekike
     1201 itkraaaesg irqglmeles weasaslqiq eakdsrnagi llvedygsll aragelrlll
     1261 egvkgatgye rfstrvarce aglyeveeri kilstvqrkw vylepvyegg aapkdtgrws
     1321 rankefrylm gevsrdpris slrrlplsaf anlkdlldrc qrsldeylee krssyprlyf
     1381 lsdedllelv sasgrgleth lpklyqgvgs iikddngltg vvspegevlq lpkpvdlrdp
     1441 lprwlsnlee amknalrqsl ekclvdatpd psayptqvll laerirfter ceialkegrs
     1501 slknfveyle tqraryrgle dagdkltvlk argllldvvh hleiarslln vtlreestvw
     1561 twqrqlrsyr tnkgpvmrca aaefpyrfey qgaavglvqt pltercflal tqamklglgg
     1621 sptgpagtgk tesvkalgai lgrtvlvfnc degmdtgsmr rilgglaqag awgcfdefnr
     1681 leeetlsava mlvrplqeav rdgspqvmlg dqkvnldphc cifitmnpag seyggrrklp
     1741 dslarlfrpi gmahpdrsdi vktllecagf ldaatlakql vetldvaeil lsnqphydwg
     1801 lralrsvlda iptrtdldet srlvaairas tlpklteedt pkflsllkdv fpkvnpstst
     1861 yierqdlqnv llelcgdqef gketanrciq lndqlksrtg vaivgppgsg ktiirkllcd
     1921 altkigekvv qfhvypgamp ksrllgrvdh qtrewkegll ssavgsagds ttwvilngdv
     1981 epdwaealns alddnrlltl pngvgvklgs gtkfifethk lddaspatvs rlgvvhlgsl
     2041 spssllvssr lgtlpeaake ltnshlclci eeclkmnadv ssasglmnsa lchlntarth
     2101 tsasyallas lcnqiedenr rnelaryiyq stdcwcpdpq kpcsvlyned idrleaagea
     2161 drstdtedgp vsltgamkea lssvlpwiks dqptmirgpe gcgksalisv ilssirsnep
     2221 stlikgssly gpqdlvmklk rgcvqlnsss hgrtykprng ssvvliledl hlaskhlqel
     2281 vrelvqeggf heddlefarv pltvvctada ttrlhprlea llsthylpyp stkdiaaise
     2341 lhlrtslkgd hivieswiaq mvpvmleafk sirsshgtaw npkdlilwad clkyypspen
     2401 etyiteylld agrrlfhprl tsrdqscwes vilsktlktl mpgsnvyiwk ggnngllpfd
     2461 eeqwrkeiin avarcaregd pvqplisshl lrtvaglswa fgtnlrgvil igrpgagrks
     2521 avrlvanfss lrlvdsgpgr grasmksavq vagidgeptl llleehhmre dglavlasai
     2581 vsrgelpglf tveeldglia pladvsrred fsgtleqyly hrlrnylrva iilnngemrs
     2641 swlsrsgllr hcaligpglg newwnreasl ielacnqnem dveeteekla gikvmvkahl
     2701 qaprqqqapa rffallhtwk hlhvswseev erklsnleag igklkeageq vakledevsk
     2761 qrqeleaeqg ranaaleqit atmrgatgqr gemvnlkaet eresaelarr kadiegelgk
     2821 veplveqaaq avagisadal aevrslrapp apvrdvlegv lrlmgikdts wnsmktflak
     2881 rgikdeirtw darrstsssl eavaklvkdr pdsfeektak rasvaaapla awvlanlqyg
     2941 qivqqvaple reqrqlaerl saaeaqigkl aaglntvesr vaklqeelae hsrgaaalql
     3001 raeatearla tarallqkld tehgdwqtql eeltgrkvkl dveaanvasl lvyedpgkdd
     3061 kwkksaidll itererllwr aqglpvdtgs lvgascalrg plvpifvdps gvavswlrnn
     3121 vgsrmeitkp edakflttle mavrfgkpll veelvefppi llpllrrrpl rlgertlpap
     3181 qgfklflatr rdrldgfpke vdavlfkial gagskslaer fvekvllket pelatqrkea
     3241 lereeklsge rdtarldlla qlatargqdl lqesegsqgg llsslqatqs kakeiamalq
     3301 esrrsfedvt rrakdhekla kfaaslyktv kaltalsply vfsaeaftdi yleaedyrgs
     3361 vlsedkreqd kaiekrlisl tlhycskavy rkhrlqlalq lalslnsvpd fekrflleev
     3421 plnddqdsdy nvpdwipeer rlsvqslvss fpqiaskmkp awlndvnniy aenslttfqk
     3481 illiktlrpd ylhtalskla aevlgvkdla ppnwsltkia eqeasypvll llspgadpgs
     3541 elrslannqv alatgfievs lgqgqvnqae laletacrng swillsnlhl alnwlprles
     3601 llrsamctin knpatriwlt teeclgfyps lsglclklay eppegvkrnv krslqqlqqk
     3661 rrnnenvpgs vllswlhatl qerrkfvpqg wirsyewsep dleaayelvv ketspkrgrd
     3721 kdgswqtgrg lldvavyggr iqdeydtral csivrdvwss qvfegrrkla gvlkvtdssf
     3781 eeairalenl pdndspkecf glpanahraw eraaaeavls hlkgvlikas isdeksnrkt
     3841 esriqrdlke lidrhgsifg ssiiegdgnn qlqrffidem kltkrtlnli radvdslrfe
     3901 dsktprhwme dwkygpkevl lfvkgllsry qtlesivsis ssvidisrla rprafltvlk
     3961 qhtaretgqp lenlrlkvnw lgnrrnegsk vslvidglli sgaliengtl kdldanaasv
     4021 aaapscqiaf lpedysenks ylgnynsedt lnvpvyansq remlicslpv ecpreehdnw
     4081 lrrgvgfhlk li
//
LOCUS       XP_034195370            4072 aa            linear   INV 03-SEP-2025
DEFINITION  dynein cytoplasmic heavy chain beethoven isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195370
VERSION     XP_034195370.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339479.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195370.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4072
                     /product="dynein cytoplasmic heavy chain beethoven isoform
                     X2"
                     /calculated_mol_wt=455844
     Region          <217..551
                     /region_name="DHC_N1"
                     /note="Dynein heavy chain, N-terminal region 1; pfam08385"
                     /db_xref="CDD:462457"
     Region          881..>1277
                     /region_name="YhaN"
                     /note="Uncharacterized conserved protein YhaN, contains
                     AAA domain [Function unknown]; COG4717"
                     /db_xref="CDD:443752"
     Region          1116..1443
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          <1335..3753
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1566..1892
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          <3932..4065
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..4072
                     /gene="btv"
                     /coded_by="XM_034339479.2:74..12292"
                     /db_xref="GeneID:117611538"
ORIGIN      
        1 mvvddrrrsl ivstaghffg vdlkdeqtir deesvldnfl drstcrtlca qpadanfegt
       61 slklsnelpv gkntlvffki eeaavteknf hdlvhvtttg sgkgstlvea lrqvwlptlr
      121 ssgldssylk kleenllgss ttasvleeed ywrkkwedak rshdktiyae tvkilksira
      181 elesaavtrd gligvedave avsgyvddlw klgdavyped rmksfldlig devvslvqnt
      241 ideirtsddr akieaismga eicekwtdma ekfttlfwph hslhpwkgtg yipercsrig
      301 krlrqiaelh aqyrqltrll tanersglgt dnlmqyfddv kvvfdadekg vnwnrlkkri
      361 eeglipaedr vaqklksqia dattpnslla efrryfelmk reglkralrg ereallsayg
      421 dlienclagp dtvnlldspr ilqeiqvars aelrleslnk lgkelladlp gydeissrvt
      481 salkeverkr qnlvdtwteg tkeavaskel tlgtdsavve ltgtsvmrvt ydprlmtlir
      541 earalssqgv efsrevatfh ntigdrmvps qrplmlttal elvravqeqs gvvwsdpqav
      601 daytarlkel vrkfaqqnse laakhsllrd lvsnllkgdt vnlignfntw ketlmnmrtt
      661 vdtveaeygn tkawklhwdr qllkalgvay rgalptllkk lteikveltf rdgslqwrpp
      721 leeiraklys airrslsipm nfrgvgdpse ahfsdliqrs gylfggvykq aeialsalet
      781 lrmkwlylaa pakidvaerl kgrspqdwtr afkdakqwaq evgrlrgnev kiwcisvdta
      841 ttrndlesas rrywerlcsd lrveassrlv aiveflssas kelerrprnv eevgvayeah
      901 arieaqssgm aeemeaivgl skvlaawtse klegvnaayt awqdltdrle rhktvvnrel
      961 edakvnlrhr avavrdeier wqakwtskpe ilsldwiism rerwtnlkeq ldalktdcqr
     1021 ivldiddile dneesikkle lqldieesnc rfqaefleel knqeeeewtv arrrlprlhd
     1081 wldswenrir vqlkdqlnee stnqrdslem dtfvgrkvre vraaiewlql lrgdeiaeeh
     1141 wgelmplldl knvknardit lghlfrsvek iqentekike itkraaaesg irqglmeles
     1201 weasaslqiq eakdsrnagi llvedygsll aragelrlll egvkgatgye rfstrvarce
     1261 aglyeveeri kilstvqrkw vylepvyegg aapkdtgrws rankefrylm gevsrdpris
     1321 slrrlplsaf anlkdlldrc qrsldeylee krssyprlyf lsdedllelv sasgrgleth
     1381 lpklyqgvgs iikddngltg vvspegevlq lpkpvdlrdp lprwlsnlee amknalrqsl
     1441 ekclvdatpd psayptqvll laerirfter ceialkegrs slknfveyle tqraryrgle
     1501 dagdkltvlk argllldvvh hleiarslln vtlreestvw twqrqlrsyr tnkgpvmrca
     1561 aaefpyrfey qgaavglvqt pltercflal tqamklglgg sptgpagtgk tesvkalgai
     1621 lgrtvlvfnc degmdtgsmr rilgglaqag awgcfdefnr leeetlsava mlvrplqeav
     1681 rdgspqvmlg dqkvnldphc cifitmnpag seyggrrklp dslarlfrpi gmahpdrsdi
     1741 vktllecagf ldaatlakql vetldvaeil lsnqphydwg lralrsvlda iptrtdldet
     1801 srlvaairas tlpklteedt pkflsllkdv fpkvnpstst yierqdlqnv llelcgdqef
     1861 gketanrciq lndqlksrtg vaivgppgsg ktiirkllcd altkigekvv qfhvypgamp
     1921 ksrllgrvdh qtrewkegll ssavgsagds ttwvilngdv epdwaealns alddnrlltl
     1981 pngvgvklgs gtkfifethk lddaspatvs rlgvvhlgsl spssllvssr lgtlpeaake
     2041 ltnshlclci eeclkmnadv ssasglmnsa lchlntarth tsasyallas lcnqiedenr
     2101 rnelaryiyq stdcwcpdpq kpcsvlyned idrleaagea drstdtedgp vsltgamkea
     2161 lssvlpwiks dqptmirgpe gcgksalisv ilssirsnep stlikgssly gpqdlvmklk
     2221 rgcvqlnsss hgrtykprng ssvvliledl hlaskhlqel vrelvqeggf heddlefarv
     2281 pltvvctada ttrlhprlea llsthylpyp stkdiaaise lhlrtslkgd hivieswiaq
     2341 mvpvmleafk sirsshgtaw npkdlilwad clkyypspen etyiteylld agrrlfhprl
     2401 tsrdqscwes vilsktlktl mpgsnvyiwk ggnngllpfd eeqwrkeiin avarcaregd
     2461 pvqplisshl lrtvaglswa fgtnlrgvil igrpgagrks avrlvanfss lrlvdsgpgr
     2521 grasmksavq vagidgeptl llleehhmre dglavlasai vsrgelpglf tveeldglia
     2581 pladvsrred fsgtleqyly hrlrnylrva iilnngemrs swlsrsgllr hcaligpglg
     2641 newwnreasl ielacnqnem dveeteekla gikvmvkahl qaprqqqapa rffallhtwk
     2701 hlhvswseev erklsnleag igklkeageq vakledevsk qrqeleaeqg ranaaleqit
     2761 atmrgatgqr gemvnlkaet eresaelarr kadiegelgk veplveqaaq avagisadal
     2821 aevrslrapp apvrdvlegv lrlmgikdts wnsmktflak rgikdeirtw darrstsssl
     2881 eavaklvkdr pdsfeektak rasvaaapla awvlanlqyg qivqqvaple reqrqlaerl
     2941 saaeaqigkl aaglntvesr vaklqeelae hsrgaaalql raeatearla tarallqkld
     3001 tehgdwqtql eeltgrkvkl dveaanvasl lvyedpgkdd kwkksaidll itererllwr
     3061 aqglpvdtgs lvgascalrg plvpifvdps gvavswlrnn vgsrmeitkp edakflttle
     3121 mavrfgkpll veelvefppi llpllrrrpl rlgertlpap qgfklflatr rdrldgfpke
     3181 vdavlfkial gagskslaer fvekvllket pelatqrkea lereeklsge rdtarldlla
     3241 qlatargqdl lqesegsqgg llsslqatqs kakeiamalq esrrsfedvt rrakdhekla
     3301 kfaaslyktv kaltalsply vfsaeaftdi yleaedyrgs vlsedkreqd kaiekrlisl
     3361 tlhycskavy rkhrlqlalq lalslnsvpd fekrflleev plnddqdsdy nvpdwipeer
     3421 rlsvqslvss fpqiaskmkp awlndvnniy aenslttfqk illiktlrpd ylhtalskla
     3481 aevlgvkdla ppnwsltkia eqeasypvll llspgadpgs elrslannqv alatgfievs
     3541 lgqgqvnqae laletacrng swillsnlhl alnwlprles llrsamctin knpatriwlt
     3601 teeclgfyps lsglclklay eppegvkrnv krslqqlqqk rrnnenvpgs vllswlhatl
     3661 qerrkfvpqg wirsyewsep dleaayelvv ketspkrgrd kdgswqtgrg lldvavyggr
     3721 iqdeydtral csivrdvwss qvfegrrkla gvlkvtdssf eeairalenl pdndspkecf
     3781 glpanahraw eraaaeavls hlkgvlikas isdeksnrkt esriqrdlke lidrhgsifg
     3841 ssiiegdgnn qlqrffidem kltkrtlnli radvdslrfe dsktprhwme dwkygpkevl
     3901 lfvkgllsry qtlesivsis ssvidisrla rprafltvlk qhtaretgqp lenlrlkvnw
     3961 lgnrrnegsk vslvidglli sgaliengtl kdldanaasv aaapscqiaf lpedysenks
     4021 ylgnynsedt lnvpvyansq remlicslpv ecpreehdnw lrrgvgfhlk li
//
LOCUS       XP_034195374            1846 aa            linear   INV 03-SEP-2025
DEFINITION  von Willebrand factor A domain-containing protein c12.2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195374
VERSION     XP_034195374.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339483.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1846
                     /product="von Willebrand factor A domain-containing
                     protein c12.2"
                     /calculated_mol_wt=207593
     Region          90..246
                     /region_name="AAA_5"
                     /note="AAA domain (dynein-related subfamily); pfam07728"
                     /db_xref="CDD:400191"
     Region          423..584
                     /region_name="AAA_5"
                     /note="AAA domain (dynein-related subfamily); pfam07728"
                     /db_xref="CDD:400191"
     Region          <705..1008
                     /region_name="PHA02244"
                     /note="ATPase-like protein"
                     /db_xref="CDD:107157"
     Region          752..898
                     /region_name="AAA_5"
                     /note="AAA domain (dynein-related subfamily); pfam07728"
                     /db_xref="CDD:400191"
     Region          <1574..>1686
                     /region_name="NorD"
                     /note="Nitric oxide reductase activation protein
                     [Inorganic ion transport and metabolism]; COG4548"
                     /db_xref="CDD:443612"
     Region          1651..1840
                     /region_name="vWFA"
                     /note="Von Willebrand factor type A (vWA) domain was
                     originally found in the blood coagulation protein von
                     Willebrand factor (vWF). Typically, the vWA domain is made
                     up of approximately 200 amino acid residues folded into a
                     classic a/b para-rossmann type of...; cl00057"
                     /db_xref="CDD:469594"
     Site            order(1658,1741,1769)
                     /site_type="other"
                     /note="metal ion-dependent adhesion site (MIDAS)"
                     /db_xref="CDD:238732"
     CDS             1..1846
                     /gene="c12.2"
                     /coded_by="XM_034339483.2:166..5706"
                     /db_xref="GeneID:117611541"
ORIGIN      
        1 mlhvncnfhr mstasqvltk skvfrsnlql firyksdnai vtignvskev kspknpeyvp
       61 rkyllkaqee dastlkhlkw mlqkdilsqd ifliggpgtr rrklalafle lterelefia
      121 lsrdtteadl kqrreirsgt ayyhdqsavr aatngrvlii egvekaernv lpvlnnllen
      181 remhledgrf lipaarydkl lethsqkeld twrlvrvsed frvialglps prypghpldp
      241 pfrsrfqarh isppsfeeql ntltslvrht dtaklrqlls cahafvtqea islglpdfpl
      301 dtlssaaiim dqcpylpvfd ifyrcypykl flgkegrsav eniletfhvl ngaqvskeis
      361 vknkiedkns invgikyane etvltvscgt krtnestnki kyvktnyqnn llvsmleshi
      421 vsdlcligpk gcgksitvqt lanlmgyqve pvvlyqdmts rdliqqrttl sngdtvwkns
      481 plvdaalqgk lavldginri dpstlailhr lvhdrelqlh dgkrlisadr ydeikekynr
      541 sdenlqdsgv lrihpsfrim alaespvint ssrqwlnsel lslflfhemr pltkseelhi
      601 irskygepst alqnilelah vlrssndptl qslsgflstr qllriagrmh kfqtddaysa
      661 vqraclarfl pavakqalqd scahvgivst ktvpntnikc titgdtvkig ntvverykta
      721 altkvpdvlf ydvpqhvall enllqdfslg qhlllvgnqg vgknkivdrl lqllnkprey
      781 iqlhrdttvq tltlqpmvrd gkvmyedspl vqavklghvl videadkapt hvtcilktlv
      841 esgemilsdg rrivssssdt qdpntialhp dfrmivlanr pgfpflghdf fgalgdlfst
      901 havdnptven eiqllkqygp nvdreiilkl vkafgelrnm adqglvsypy strevvnivk
      961 hlekfpqesl anvvrnvfdf dryseetfnt lvsilhkhgi pigtsptnia lakeislpsn
     1021 kicatwnvsn qqqllrvqek yiklkssikl rqntlpldlv earsalftel qsywtvplgn
     1081 sgvvaaltiv kgtrddgkdd kinilaenpl kiftmslesd tiqetllpsl isyerhnkpf
     1141 ytmasdnnqn vliheeisnt lllvntinrt vqkiellsfl dmasdklsna frtnnlrwkm
     1201 ktdliqshnq ivfytpnskr ievidlhsmc sysinlpfdi esivlpsekk wliqdtrrnk
     1261 yilkrsadss pcpnvlqele eiskdnvkvg aflscsntnl ssnvlsaale qkiespnrll
     1321 andntyatia vgfpdldsfn elhfwprphr srsftspivm engqiiravi peevpddvlp
     1381 ktgkhlgivg yleivdtvnh kmrylpipep vnvsyatqll ypnnyplyis sksnqdlvtv
     1441 daggcvrlwe aslasieksl gawrrmigdd nenlqitker ysgldvsspk hgkidqqnap
     1501 hvggntwagg tggrdtaglg gkggpyrlda ghtvhqlsde eknavpehik kaamemgrka
     1561 fqqrlrdinm seydhnlysq fsnavqkqvk alrtiidslq akskerqwcr hqtsgelddt
     1621 kliegltger tiyrrraeke pdpgapltkp krlklvvdvs gsmyrfngyd grldremeas
     1681 vmvmeafsgy egkfqydivg hsgddydivf vkhtqppadn krrleviktm hahsqfcmsg
     1741 dntlqatqqa iaslakedsd esivvvfsda nfdrygirpe sfakiltsnp nvnafaifig
     1801 slgnqavslt krmpsgrafv cmdlkdipri lqqifaasll sttrss
//
LOCUS       XP_034195375            1397 aa            linear   INV 03-SEP-2025
DEFINITION  SH2 domain-containing adapter heavyweight [Osmia lignaria
            lignaria].
ACCESSION   XP_034195375
VERSION     XP_034195375.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339484.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195375.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1397
                     /product="SH2 domain-containing adapter heavyweight"
                     /calculated_mol_wt=156008
     Region          1294..1392
                     /region_name="SH2_SHB_SHD_SHE_SHF_like"
                     /note="Src homology 2 domain found in SH2
                     domain-containing adapter proteins B, D, E, and F (SHB,
                     SHD, SHE, SHF); cd09945"
                     /db_xref="CDD:198198"
     Site            order(1303,1321,1342,1344)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198198"
     Site            order(1343,1373)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198198"
     CDS             1..1397
                     /gene="hwt"
                     /coded_by="XM_034339484.2:86..4279"
                     /db_xref="GeneID:117611542"
ORIGIN      
        1 mervwrrvqg agrrgatarc gcpvqpcqlh vdqrakrggg rksrggpqti vcscqvqpcp
       61 vhpvlirvrr trptvgncdc ppesqpcvha qrasavrrir rcdcperpcr htapagtrhr
      121 vhsssptfsq asqstsqqqq qqqcecveri ncthtvagdg srececgeev kpcshtvrqr
      181 rsrrscdcsg sgsqpcshkp qhegrikrvk crvrragcam arctaelaml hlhwglatec
      241 apckpraltr rlcrrqqsdn elyrsnsfkf erfertkdec stplrkqvsl cddyslpvdf
      301 vnkrrpasaa aatstvqwal psptienaev evveqysdpr dsknkayvep gaetslptiy
      361 skvnlnycrp ytdpsasgss cedddddrhp dirkrknsvy atsstratsl drekelsftd
      421 aalvdcvalv hltdqsptds nqpvrhpspy yygdlfkvpe qlskpqpnky rksvsldvpg
      481 ersrtpgipk rnsvagdggs yssqpqlqpq qqqqqqqqpl qpqqhyqsed lvsptsgseh
      541 avparneils sciitewdht lmpphrllsh dlpttvctcv asaeeeedel dqrqpqltrh
      601 qvrklrrtrr idpqpilved eddvegedeg eeeeeedvev eedeeemarq rhlyetafdc
      661 kvnrsdddld dldrvtnhpv lhsqmrsnsa iavsrtssst dtskqqqpqq ipyagqshss
      721 kdrrkvvllr pkpipsrlqq qqqqqqqqqq qqqqqsdnis tlsqdieslq inssdektgs
      781 prlpargytp sppstaplpa kfhgnrdhll lnirstpnlp sqpdhprlkd lrlpvksllr
      841 akdspqsseg sileiksrpk sfaqdnvess qgrrfdkeli lefkgrpree rqrprslire
      901 skpimefkgr pheaesdllr prrevgllef klrttrrrag ysstesmats ssggsmeslr
      961 sstsegnrst sssesrhsts lsshssdsgs tncyhhhqqp smtgflthha nklhilspis
     1021 dkssqepase tsdnnrnnns qkaspeenvt tennvtvsnn tntntntntn tntantntns
     1081 vtspmdtsfk krrtpqnknl inlalqssss gdaeiqgsds gisiesragi kckpfgfhll
     1141 kpqldninlv dnepelsdlp fdmpklrrrr llmqqdatts gsatsvdlrd lpfdmpklrr
     1201 rlrcmqstes sgsqasssls vrdveppalf ggglarpkmt lnldagngvn nggngggqia
     1261 pkkpgglslg lpleintarg sadlvdvdlp lerqgwyhgs itrieaeavl rllregsylv
     1321 rnsestkqdy slslksargf mhmriqknee lnayilgqfs kpfdsipemv rhfsvnrlpi
     1381 rgaehmcllh pviaqll
//
LOCUS       XP_034195377            1326 aa            linear   INV 03-SEP-2025
DEFINITION  retinal degeneration A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195377
VERSION     XP_034195377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1326
                     /product="retinal degeneration A isoform X1"
                     /calculated_mol_wt=145875
     Region          387..443
                     /region_name="C1_DGK_typeIV_rpt1"
                     /note="first protein kinase C conserved region 1 (C1
                     domain) found in type IV diacylglycerol kinases; cd20802"
                     /db_xref="CDD:410352"
     Region          457..518
                     /region_name="C1_DGK_typeIV_rpt2"
                     /note="second protein kinase C conserved region 1 (C1
                     domain) found in type IV diacylglycerol kinases; cd20855"
                     /db_xref="CDD:410405"
     Region          593..714
                     /region_name="DAGKc"
                     /note="Diacylglycerol kinase catalytic domain (presumed);
                     smart00046"
                     /db_xref="CDD:214487"
     Region          742..896
                     /region_name="DAGK_acc"
                     /note="Diacylglycerol kinase accessory domain; cl02440"
                     /db_xref="CDD:470579"
     Region          <1036..1127
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          <1196..1309
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          1221..1253
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1222,1226..1227,1230..1232,1234..1235,1239,1242,
                     1253,1255,1258,1262..1263,1266..1268,1270..1271,1275,1278,
                     1287,1289,1291,1295..1296,1299..1301,1303..1304,1308,1311)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1255..1287
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1326
                     /gene="rdgA"
                     /coded_by="XM_034339486.2:1761..5741"
                     /db_xref="GeneID:117611543"
ORIGIN      
        1 mqrlrstfkr srtptgaemk sqsslevpkq vrsasfdeiq leakrqddir darstrttss
       61 sssscspaks qdssrgrras tlrvpqlqsg qrsksfdvce rsygssssqq slgsirrpet
      121 ptiqvsgcyh cacveeyknl yssrdedeps sredeevdeg edtsddedse lidrsegspe
      181 irvtlttfte kgadvtdtvp evvspvvpei vpevarddtd tsavrgelfp erqrrrslas
      241 pklarqealt sypidlptvv sstedeekle ddeddeevan vetnkskivv rdifltvpel
      301 krdraasvds cfnnkngngg dadtlavpqq nirsksvdiv lptdaqtryt allpgtearp
      361 prggreeggt sgghreprst pdwgpgalng ehlwvptsas gdfcyvndcs khgarmkcaa
      421 crvvahtvca tslnfackss frdvgvrqyr eqpntqhhwv hrrsqkgkcg ncgksfqskl
      481 sftskeivav ncswckrayh nkeacfnvqk igencelgah ssiivppswi vklprkgsfk
      541 sslrksprkk ksggggstgs rkkskekdke kdqdqgklwv vkpiptasvk pvlvfinpks
      601 ggnqgakllq kfqwllnprq vfdltqggpk mglelfkkvp nlrvlacggd gtvgwvlsil
      661 dqigaspppa vgvlplgtgn dlaralgwgg gytdepigki ltnigesdtt lldrwelive
      721 rnpdaqgddd ngkgkenlpl nvvnnyyslg vdahialefh eareahpekf nsrlrnkmyy
      781 gqmggkdlvl rkwkdlsefv tlecdgqdvt pklkehrvha ivflniasyg ggthpwgaas
      841 gtkepstedg iievvgltty qlpllqaggh gtciaqcsta klvttktipm qvdgeacrll
      901 psiinmklln katmlakrrn askppqdvkl drlnlpvmki kmsdyekyhh dkdmlknaas
      961 twiadpldld attdleslrt ilakdhnmdt ccfldsctae rffridraqe qlhyvtdvav
     1021 evvyvleesa nnqstepegk vsasqepeta hpspseerpk snaamtqeqp kseskaqpsp
     1081 vpesetaekp aedtktqaet qdtakcqgsm kknmardepq ntkkeekane gkvsrdavke
     1141 sgssksisvt tvaqsqhqap ptfisprdrl fglssevhtf ntrllektsd gilktaklgn
     1201 iqalkelher gyslmcidat gqtalhlasr yghkdivryl iacapssiln midndkgqta
     1261 lhkaaqykrr svccmlvagg anltikdrqg ntprdlalqa edrelaayly sqeqfqltte
     1321 emenal
//
LOCUS       XP_076546570            1278 aa            linear   INV 03-SEP-2025
DEFINITION  retinal degeneration A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546570
VERSION     XP_076546570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690455.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1278
                     /product="retinal degeneration A isoform X2"
                     /calculated_mol_wt=140501
     Region          387..443
                     /region_name="C1_DGK_typeIV_rpt1"
                     /note="first protein kinase C conserved region 1 (C1
                     domain) found in type IV diacylglycerol kinases; cd20802"
                     /db_xref="CDD:410352"
     Region          457..518
                     /region_name="C1_DGK_typeIV_rpt2"
                     /note="second protein kinase C conserved region 1 (C1
                     domain) found in type IV diacylglycerol kinases; cd20855"
                     /db_xref="CDD:410405"
     Region          593..714
                     /region_name="DAGKc"
                     /note="Diacylglycerol kinase catalytic domain (presumed);
                     smart00046"
                     /db_xref="CDD:214487"
     Region          742..896
                     /region_name="DAGK_acc"
                     /note="Diacylglycerol kinase accessory domain; cl02440"
                     /db_xref="CDD:470579"
     Region          <1036..1127
                     /region_name="PspC_subgroup_2"
                     /note="pneumococcal surface protein PspC, LPXTG-anchored
                     form; NF033839"
                     /db_xref="CDD:468202"
     Region          1196..>1257
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     CDS             1..1278
                     /gene="rdgA"
                     /coded_by="XM_076690455.1:2846..6682"
                     /db_xref="GeneID:117611543"
ORIGIN      
        1 mqrlrstfkr srtptgaemk sqsslevpkq vrsasfdeiq leakrqddir darstrttss
       61 sssscspaks qdssrgrras tlrvpqlqsg qrsksfdvce rsygssssqq slgsirrpet
      121 ptiqvsgcyh cacveeyknl yssrdedeps sredeevdeg edtsddedse lidrsegspe
      181 irvtlttfte kgadvtdtvp evvspvvpei vpevarddtd tsavrgelfp erqrrrslas
      241 pklarqealt sypidlptvv sstedeekle ddeddeevan vetnkskivv rdifltvpel
      301 krdraasvds cfnnkngngg dadtlavpqq nirsksvdiv lptdaqtryt allpgtearp
      361 prggreeggt sgghreprst pdwgpgalng ehlwvptsas gdfcyvndcs khgarmkcaa
      421 crvvahtvca tslnfackss frdvgvrqyr eqpntqhhwv hrrsqkgkcg ncgksfqskl
      481 sftskeivav ncswckrayh nkeacfnvqk igencelgah ssiivppswi vklprkgsfk
      541 sslrksprkk ksggggstgs rkkskekdke kdqdqgklwv vkpiptasvk pvlvfinpks
      601 ggnqgakllq kfqwllnprq vfdltqggpk mglelfkkvp nlrvlacggd gtvgwvlsil
      661 dqigaspppa vgvlplgtgn dlaralgwgg gytdepigki ltnigesdtt lldrwelive
      721 rnpdaqgddd ngkgkenlpl nvvnnyyslg vdahialefh eareahpekf nsrlrnkmyy
      781 gqmggkdlvl rkwkdlsefv tlecdgqdvt pklkehrvha ivflniasyg ggthpwgaas
      841 gtkepstedg iievvgltty qlpllqaggh gtciaqcsta klvttktipm qvdgeacrll
      901 psiinmklln katmlakrrn askppqdvkl drlnlpvmki kmsdyekyhh dkdmlknaas
      961 twiadpldld attdleslrt ilakdhnmdt ccfldsctae rffridraqe qlhyvtdvav
     1021 evvyvleesa nnqstepegk vsasqepeta hpspseerpk snaamtqeqp kseskaqpsp
     1081 vpesetaekp aedtktqaet qdtakcqgsm kknmardepq ntkkeekane gkvsrdavke
     1141 sgssksisvt tvaqsqhqap ptfisprdrl fglssevhtf ntrllektsd gilktaklgn
     1201 iqalkelher gyslmcidat gqtalhlasr yghkdivryl iacapssiln midndksrav
     1261 padngrnger pltygrnr
//
LOCUS       XP_034195378            1073 aa            linear   INV 03-SEP-2025
DEFINITION  retinal degeneration A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195378
VERSION     XP_034195378.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339487.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1073
                     /product="retinal degeneration A isoform X3"
                     /calculated_mol_wt=118133
     Region          387..443
                     /region_name="C1_DGK_typeIV_rpt1"
                     /note="first protein kinase C conserved region 1 (C1
                     domain) found in type IV diacylglycerol kinases; cd20802"
                     /db_xref="CDD:410352"
     Region          457..518
                     /region_name="C1_DGK_typeIV_rpt2"
                     /note="second protein kinase C conserved region 1 (C1
                     domain) found in type IV diacylglycerol kinases; cd20855"
                     /db_xref="CDD:410405"
     Region          593..714
                     /region_name="DAGKc"
                     /note="Diacylglycerol kinase catalytic domain (presumed);
                     smart00046"
                     /db_xref="CDD:214487"
     Region          742..896
                     /region_name="DAGK_acc"
                     /note="Diacylglycerol kinase accessory domain; cl02440"
                     /db_xref="CDD:470579"
     CDS             1..1073
                     /gene="rdgA"
                     /coded_by="XM_034339487.2:2846..6067"
                     /db_xref="GeneID:117611543"
ORIGIN      
        1 mqrlrstfkr srtptgaemk sqsslevpkq vrsasfdeiq leakrqddir darstrttss
       61 sssscspaks qdssrgrras tlrvpqlqsg qrsksfdvce rsygssssqq slgsirrpet
      121 ptiqvsgcyh cacveeyknl yssrdedeps sredeevdeg edtsddedse lidrsegspe
      181 irvtlttfte kgadvtdtvp evvspvvpei vpevarddtd tsavrgelfp erqrrrslas
      241 pklarqealt sypidlptvv sstedeekle ddeddeevan vetnkskivv rdifltvpel
      301 krdraasvds cfnnkngngg dadtlavpqq nirsksvdiv lptdaqtryt allpgtearp
      361 prggreeggt sgghreprst pdwgpgalng ehlwvptsas gdfcyvndcs khgarmkcaa
      421 crvvahtvca tslnfackss frdvgvrqyr eqpntqhhwv hrrsqkgkcg ncgksfqskl
      481 sftskeivav ncswckrayh nkeacfnvqk igencelgah ssiivppswi vklprkgsfk
      541 sslrksprkk ksggggstgs rkkskekdke kdqdqgklwv vkpiptasvk pvlvfinpks
      601 ggnqgakllq kfqwllnprq vfdltqggpk mglelfkkvp nlrvlacggd gtvgwvlsil
      661 dqigaspppa vgvlplgtgn dlaralgwgg gytdepigki ltnigesdtt lldrwelive
      721 rnpdaqgddd ngkgkenlpl nvvnnyyslg vdahialefh eareahpekf nsrlrnkmyy
      781 gqmggkdlvl rkwkdlsefv tlecdgqdvt pklkehrvha ivflniasyg ggthpwgaas
      841 gtkepstedg iievvgltty qlpllqaggh gtciaqcsta klvttktipm qvdgeacrll
      901 psiinmklln katmlakrrn askppqdvkl drlnlpvmki kmsdyekyhh dkdmlknaas
      961 twiadpldld attdleslrt ilakdhnmdt ccfldsctae rffridraqe qlhyvtdvav
     1021 evvyvleesa nnqstepegk vsasqepeta hpsprliert cniakngqnq mqp
//
LOCUS       XP_034195380            1157 aa            linear   INV 03-SEP-2025
DEFINITION  3'-5' RNA helicase YTHDC2 [Osmia lignaria lignaria].
ACCESSION   XP_034195380
VERSION     XP_034195380.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1157
                     /product="3'-5' RNA helicase YTHDC2"
                     /calculated_mol_wt=131367
     Region          18..77
                     /region_name="R3H"
                     /note="R3H domain. The name of the R3H domain comes from
                     the characteristic spacing of the most conserved arginine
                     and histidine residues. R3H domains are found in proteins
                     together with ATPase domains, SF1 helicase domains, SF2
                     DEAH helicase domains, Cys-rich...; cl00297"
                     /db_xref="CDD:469711"
     Site            order(48,52)
                     /site_type="other"
                     /note="RxxxH motif"
                     /db_xref="CDD:100064"
     Region          160..>978
                     /region_name="DEAH_box_HrpA"
                     /note="RNA helicase HrpA; TIGR01967"
                     /db_xref="CDD:273900"
     Region          166..341
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Site            order(191..197,289)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     Region          932..1028
                     /region_name="OB_NTP_bind"
                     /note="Oligonucleotide/oligosaccharide-binding (OB)-fold;
                     pfam07717"
                     /db_xref="CDD:400182"
     CDS             1..1157
                     /gene="LOC117611544"
                     /coded_by="XM_034339489.2:315..3788"
                     /db_xref="GeneID:117611544"
ORIGIN      
        1 mprrnrhkkt pligedtria vnltlkklle tpdqkelefp ssytaeeray ihklshelgl
       61 ksksrgkgtn rfltvykreg stivqadavi klqkasrqsi ynllqtfpln hkecqdllpp
      121 iererplnae vsvststkam grlnnsvpqi pqlktnfdvl nyrkalpifn sredilnaln
      181 snqvviigge tgsgkttqvp qfilehcqqr hqlcriictq prrlsavsva ervaferdek
      241 igqtfgyqir lesrvapktl ltyctngvll rtlmagdsal stvthvivde iherdrfcdf
      301 llialkdalq khkslklilm satldvsvfv kyfskcavin vlgrsydvdt yfledvlkit
      361 gymtkemlsk rkellnkkdq rklleswtqy kpqqlgshtg nercllpapi laqqneplpe
      421 kiklepwlie emdksiseaw lrggednfaq llyfilsenv svdyqhstts vtplmvaagr
      481 gcintteqll nlganlnlra gnewtaldwa rnmnqtecae lieaymktyd ctvqddtith
      541 akdvslseed kllldvyhht fnddninydl llqlimhihl kmplgsllif lpgyddivtm
      601 rekinneeke mnqglryvly ilhsnmqtcd qkkvfkpspm gtrkiilstn iaetsitidd
      661 vvyvidsgkv keksfdaisg vctlksnwis qacakqrkgr agrcrkgicy rlfssvryns
      721 mqpyqtpeil rlplqelcly tkhlapgntp iaeyldraie ppsnmvtrna vqllktidal
      781 dpwedltelg shlldlpiep rlgkmllyav vlkcldpvlt ivcslaykdp fvlpsqpsqk
      841 raataarkrf atntysdhma vlrafqgwqn arasgkeraf ceqnfisaav memvvgmrtq
      901 llgqlrasgf vrargpgdir dlnsnsenwa vvkaaltagl ypnlirvdre hlqlrtqkev
      961 kvffhpsstl rdypksprmt saqthatnve slpcdwllye emsrtgrfch vrmatlvnpl
     1021 tvalfcgpar lsvdviyeae svpesesdse adennegtif klddwvvfkl dpetarfflh
     1081 lrqkwnalfl rrmktpnkam sqldekvvst lvnvitneeq acglqqpsgi gqrprplivd
     1141 yypanarrtd dfvernf
//
LOCUS       XP_034195382             901 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611545 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195382
VERSION     XP_034195382.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339491.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195382.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..901
                     /product="uncharacterized protein LOC117611545 isoform X1"
                     /calculated_mol_wt=100574
     Region          <242..600
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          504..675
                     /region_name="DUF4795"
                     /note="Domain of unknown function (DUF4795); pfam16043"
                     /db_xref="CDD:464990"
     CDS             1..901
                     /gene="LOC117611545"
                     /coded_by="XM_034339491.2:665..3370"
                     /db_xref="GeneID:117611545"
ORIGIN      
        1 msaetakapv atpanpapdd iaslqvslpq mldlalgtpe vgvvnlnilh hflhillhqi
       61 nlrttkveyr gedanriktm vsslkagpsl hlqeysiisg andvkqrvqd gepanlnvdv
      121 vvdgpvetpa vktgsgsvae seksviekei msthsqadad lktakgtrqk ispgvngepe
      181 tviyveplvd gstptalgfk rleqsvnelr hqfhvleela ttpelvervk gkvtdpvtdv
      241 wqiinitkrl dateqgidkl tkmmqdvmkg disfegeets qlddrlanve idlenlnriv
      301 knlqiisdsa edngeeillq eapstakeng qpgqrvslsq llggidvkqm nqdvaslqgq
      361 vtqmkeelqn lntkihksks eliqhvakeq akykeqelsk ksqvdidasa eeseleekse
      421 kyaegvketp ketkpapkda skekqmestp rkstseaveg adaidseqlq emrerigkle
      481 kdvsclfekv esapmagvsg naelddlvsk iqgiqtdmdk lnqtadnlid drenreihln
      541 alleqvellk tikadredle daladkadaq aitrkvsydq fdaacddlaq gledainklg
      601 kqesiwqqam devqreiegk vdkmemtplk dfvsaklksl qekvknvaqm rqeseaagtk
      661 kllkdvqcis cdknvvmkme dshrfrsetl pctgsmkpyl tyeldqvrkq hrrlphsrnm
      721 lqfeaalqed arkqrsakad tlvktprdhl cnrycggsht vttpqqrvmr vghfltqwgp
      781 eiiqltegmi kgtdgkmyks rpmpgkfdvc gpgycqdrgd eirlsmkvps tpqdqrkise
      841 kqsngrkkts rrssreltte vieelpetpr erdrnaseft tyetsdrgvr ymneddmedl
      901 e
//
LOCUS       XP_076546571             901 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611545 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546571
VERSION     XP_076546571.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690456.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..901
                     /product="uncharacterized protein LOC117611545 isoform X1"
                     /calculated_mol_wt=100574
     Region          <242..600
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          504..675
                     /region_name="DUF4795"
                     /note="Domain of unknown function (DUF4795); pfam16043"
                     /db_xref="CDD:464990"
     CDS             1..901
                     /gene="LOC117611545"
                     /coded_by="XM_076690456.1:344..3049"
                     /db_xref="GeneID:117611545"
ORIGIN      
        1 msaetakapv atpanpapdd iaslqvslpq mldlalgtpe vgvvnlnilh hflhillhqi
       61 nlrttkveyr gedanriktm vsslkagpsl hlqeysiisg andvkqrvqd gepanlnvdv
      121 vvdgpvetpa vktgsgsvae seksviekei msthsqadad lktakgtrqk ispgvngepe
      181 tviyveplvd gstptalgfk rleqsvnelr hqfhvleela ttpelvervk gkvtdpvtdv
      241 wqiinitkrl dateqgidkl tkmmqdvmkg disfegeets qlddrlanve idlenlnriv
      301 knlqiisdsa edngeeillq eapstakeng qpgqrvslsq llggidvkqm nqdvaslqgq
      361 vtqmkeelqn lntkihksks eliqhvakeq akykeqelsk ksqvdidasa eeseleekse
      421 kyaegvketp ketkpapkda skekqmestp rkstseaveg adaidseqlq emrerigkle
      481 kdvsclfekv esapmagvsg naelddlvsk iqgiqtdmdk lnqtadnlid drenreihln
      541 alleqvellk tikadredle daladkadaq aitrkvsydq fdaacddlaq gledainklg
      601 kqesiwqqam devqreiegk vdkmemtplk dfvsaklksl qekvknvaqm rqeseaagtk
      661 kllkdvqcis cdknvvmkme dshrfrsetl pctgsmkpyl tyeldqvrkq hrrlphsrnm
      721 lqfeaalqed arkqrsakad tlvktprdhl cnrycggsht vttpqqrvmr vghfltqwgp
      781 eiiqltegmi kgtdgkmyks rpmpgkfdvc gpgycqdrgd eirlsmkvps tpqdqrkise
      841 kqsngrkkts rrssreltte vieelpetpr erdrnaseft tyetsdrgvr ymneddmedl
      901 e
//
LOCUS       XP_076546572             822 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611545 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546572
VERSION     XP_076546572.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690457.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..822
                     /product="uncharacterized protein LOC117611545 isoform X2"
                     /calculated_mol_wt=92001
     Region          <163..521
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          425..596
                     /region_name="DUF4795"
                     /note="Domain of unknown function (DUF4795); pfam16043"
                     /db_xref="CDD:464990"
     CDS             1..822
                     /gene="LOC117611545"
                     /coded_by="XM_076690457.1:275..2743"
                     /db_xref="GeneID:117611545"
ORIGIN      
        1 mvsslkagps lhlqeysiis gandvkqrvq dgepanlnvd vvvdgpvetp avktgsgsva
       61 eseksvieke imsthsqada dlktakgtrq kispgvngep etviyveplv dgstptalgf
      121 krleqsvnel rhqfhvleel attpelverv kgkvtdpvtd vwqiinitkr ldateqgidk
      181 ltkmmqdvmk gdisfegeet sqlddrlanv eidlenlnri vknlqiisds aedngeeill
      241 qeapstaken gqpgqrvsls qllggidvkq mnqdvaslqg qvtqmkeelq nlntkihksk
      301 seliqhvake qakykeqels kksqvdidas aeeseleeks ekyaegvket pketkpapkd
      361 askekqmest prkstseave gadaidseql qemrerigkl ekdvsclfek vesapmagvs
      421 gnaelddlvs kiqgiqtdmd klnqtadnli ddrenreihl nalleqvell ktikadredl
      481 edaladkada qaitrkvsyd qfdaacddla qgledainkl gkqesiwqqa mdevqreieg
      541 kvdkmemtpl kdfvsaklks lqekvknvaq mrqeseaagt kkllkdvqci scdknvvmkm
      601 edshrfrset lpctgsmkpy ltyeldqvrk qhrrlphsrn mlqfeaalqe darkqrsaka
      661 dtlvktprdh lcnrycggsh tvttpqqrvm rvghfltqwg peiiqltegm ikgtdgkmyk
      721 srpmpgkfdv cgpgycqdrg deirlsmkvp stpqdqrkis ekqsngrkkt srrssreltt
      781 evieelpetp rerdrnasef ttyetsdrgv rymneddmed le
//
LOCUS       XP_034195384             708 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611546 [Osmia lignaria lignaria].
ACCESSION   XP_034195384
VERSION     XP_034195384.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339493.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="uncharacterized protein LOC117611546"
                     /calculated_mol_wt=80252
     Region          40..289
                     /region_name="STKc_MLCK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase; cd14103"
                     /db_xref="CDD:271005"
     Site            order(40..44,46,48,61,63,92,108..109,111,115,117,155,157,
                     159..160,162,174..175,178,193..196)
                     /site_type="active"
                     /db_xref="CDD:271005"
     Site            order(40..44,46,48,61,63,92,108..109,111,115,159..160,162,
                     174..175)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271005"
     Site            order(44,115,117,155,157,159,178,193..196)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271005"
     Site            order(174..182,185..196)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271005"
     CDS             1..708
                     /gene="LOC117611546"
                     /coded_by="XM_034339493.2:218..2344"
                     /db_xref="GeneID:117611546"
ORIGIN      
        1 mirvdesdpv geiepsfpyr dvtvrrgvef kdyydiqsei grgkfgtvyr ckekasglml
       61 aakvvntskk edrrsverev eimrrlqhpr liqlydaidt gkqihvilel ieggelferv
      121 idddfvlter sctvfmrqic egiefihrqn ilhldlkpen ilcltkegnr ikiidfglar
      181 eydpkkklqv lfgtpefvap evvnfdqigf gtdmwsigvi cyvllsglsp fmgdtdietm
      241 anvtiakydf dhdafadisd dakdfircll vkdkekrtsa aqcrehrwla kkpskqqveq
      301 mvsksksnvp qqiheiprle msnaeeldva kdnlrlfver wrehpnspym ldippynflq
      361 nstkdhddrv slkghspspc asicstpset tdnsprgsyg sfltvpgfgf errasegiat
      421 eksrgdpasq iilaeeiikl sehlrsiamg tciksndedv rsldsqekkt ekpeeksarn
      481 gvhsksnglm saensdcnei tekltsimrh rklngttfhs ssrnfekyte tnsgnifasl
      541 kkqkqnader scsrkeqdrt seetatssvr sqsvqekveg ttreknvrls adgemdltpp
      601 wrrsrvkhlf getsrdvpri selrnlhksl nldestntkd lllhlleewd gvatrssgvg
      661 rksvsvdwcg eesvgrktmn slaeyfqakq qkstassvns taqnsvhr
//
LOCUS       XP_076546573             405 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546573
VERSION     XP_076546573.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690458.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="troponin T, skeletal muscle isoform X1"
                     /calculated_mol_wt=48568
     Region          166..>232
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..405
                     /gene="TpnT"
                     /coded_by="XM_076690458.1:164..1381"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqyss eeevveetqp erkiegrasq kesenpefmk rqeqkrsdld eqlkeyiaew
       61 rkqrakeeee lkrlkekqak rkitradeek rlaqkkkeee errqreieek kqrdieekrk
      121 rleesekkrq ammqamkdqs kkgpnftitr kdlagnltsa qlernktkeq leeekkisls
      181 irikpleidg lsieklrska nelwdtivkl etekydleer qkrqdydlke lkerqkqqlr
      241 hkalkkgldp ealtgkyppk iqvaskyerr vdtrsyddkk klfegglteq qkeiiekqwa
      301 qqkqqflgrq ktklpkwfge rpgkkpgdpe spegeedvka aaedeeleep qfdeeeeeee
      361 eeeeeeeeeg ekkgegegee eeeeeeeeee eeeeeeeeee eeeee
//
LOCUS       XP_076546574             405 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546574
VERSION     XP_076546574.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690459.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="troponin T, skeletal muscle isoform X1"
                     /calculated_mol_wt=48568
     Region          166..>232
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..405
                     /gene="TpnT"
                     /coded_by="XM_076690459.1:130..1347"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqyss eeevveetqp erkiegrasq kesenpefmk rqeqkrsdld eqlkeyiaew
       61 rkqrakeeee lkrlkekqak rkitradeek rlaqkkkeee errqreieek kqrdieekrk
      121 rleesekkrq ammqamkdqs kkgpnftitr kdlagnltsa qlernktkeq leeekkisls
      181 irikpleidg lsieklrska nelwdtivkl etekydleer qkrqdydlke lkerqkqqlr
      241 hkalkkgldp ealtgkyppk iqvaskyerr vdtrsyddkk klfegglteq qkeiiekqwa
      301 qqkqqflgrq ktklpkwfge rpgkkpgdpe spegeedvka aaedeeleep qfdeeeeeee
      361 eeeeeeeeeg ekkgegegee eeeeeeeeee eeeeeeeeee eeeee
//
LOCUS       XP_076546575             405 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546575
VERSION     XP_076546575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690460.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="troponin T, skeletal muscle isoform X2"
                     /calculated_mol_wt=48582
     Region          <21..>245
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          166..>232
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..405
                     /gene="TpnT"
                     /coded_by="XM_076690460.1:164..1381"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqyss eeevveetqp erkiegrasq kesenpefmk rqeqkrsdld eqlkeyiaew
       61 rkqrakeeee lkrlkekqak rkitradeek rlaqkkkeee errqreieek kqrdieekrk
      121 rleesekkrq ammqamkdqs kkgpnftitr kdlagnltsa qlernktkeq leeekkisls
      181 irikpleidg lsieklrska nelwdtivkl etekydleer qkrqdydlke lkerqkqqlr
      241 hkalkkgldp ealtgkyppk iqvaskyerr vdtrsyddkk klfeggydtl laeineklwk
      301 qkseqfmkrt ktklpkwfge rpgkkpgdpe spegeedvka aaedeeleep qfdeeeeeee
      361 eeeeeeeeeg ekkgegegee eeeeeeeeee eeeeeeeeee eeeee
//
LOCUS       XP_076546576             399 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546576
VERSION     XP_076546576.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690461.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="troponin T, skeletal muscle isoform X3"
                     /calculated_mol_wt=47941
     Region          160..>226
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..399
                     /gene="TpnT"
                     /coded_by="XM_076690461.1:164..1363"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqyss eeevveetqp erkieesenp efmkrqeqkr sdldeqlkey iaewrkqrak
       61 eeeelkrlke kqakrkitra deekrlaqkk keeeerrqre ieekkqrdie ekrkrleese
      121 kkrqammqam kdqskkgpnf titrkdlagn ltsaqlernk tkeqleeekk islsirikpl
      181 eidglsiekl rskanelwdt ivkletekyd leerqkrqdy dlkelkerqk qqlrhkalkk
      241 gldpealtgk yppkiqvask yerrvdtrsy ddkkklfegg lteqqkeiie kqwaqqkqqf
      301 lgrqktklpk wfgerpgkkp gdpespegee dvkaaaedee leepqfdeee eeeeeeeeee
      361 eeegekkgeg egeeeeeeee eeeeeeeeee eeeeeeeee
//
LOCUS       XP_076546577             396 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546577
VERSION     XP_076546577.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690462.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="troponin T, skeletal muscle isoform X4"
                     /calculated_mol_wt=47536
     Region          <1..>236
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          157..>223
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..396
                     /gene="TpnT"
                     /coded_by="XM_076690462.1:164..1354"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqysq perkiegras qkesenpefm krqeqkrsdl deqlkeyiae wrkqrakeee
       61 elkrlkekqa krkitradee krlaqkkkee eerrqreiee kkqrdieekr krleesekkr
      121 qammqamkdq skkgpnftit rkdlagnlts aqlernktke qleeekkisl sirikpleid
      181 glsieklrsk anelwdtivk letekydlee rqkrqdydlk elkerqkqql rhkalkkgld
      241 pealtgkypp kiqvaskyer rvdtrsyddk kklfegglte qqkeiiekqw aqqkqqflgr
      301 qktklpkwfg erpgkkpgdp espegeedvk aaaedeelee pqfdeeeeee eeeeeeeeee
      361 gekkgegege eeeeeeeeee eeeeeeeeee eeeeee
//
LOCUS       XP_076546578             396 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546578
VERSION     XP_076546578.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690463.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="troponin T, skeletal muscle isoform X4"
                     /calculated_mol_wt=47536
     Region          <1..>236
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          157..>223
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..396
                     /gene="TpnT"
                     /coded_by="XM_076690463.1:124..1314"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqysq perkiegras qkesenpefm krqeqkrsdl deqlkeyiae wrkqrakeee
       61 elkrlkekqa krkitradee krlaqkkkee eerrqreiee kkqrdieekr krleesekkr
      121 qammqamkdq skkgpnftit rkdlagnlts aqlernktke qleeekkisl sirikpleid
      181 glsieklrsk anelwdtivk letekydlee rqkrqdydlk elkerqkqql rhkalkkgld
      241 pealtgkypp kiqvaskyer rvdtrsyddk kklfegglte qqkeiiekqw aqqkqqflgr
      301 qktklpkwfg erpgkkpgdp espegeedvk aaaedeelee pqfdeeeeee eeeeeeeeee
      361 gekkgegege eeeeeeeeee eeeeeeeeee eeeeee
//
LOCUS       XP_076546579             390 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546579
VERSION     XP_076546579.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690464.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="troponin T, skeletal muscle isoform X5"
                     /calculated_mol_wt=46909
     Region          151..>217
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..390
                     /gene="TpnT"
                     /coded_by="XM_076690464.1:164..1336"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqysq perkieesen pefmkrqeqk rsdldeqlke yiaewrkqra keeeelkrlk
       61 ekqakrkitr adeekrlaqk kkeeeerrqr eieekkqrdi eekrkrlees ekkrqammqa
      121 mkdqskkgpn ftitrkdlag nltsaqlern ktkeqleeek kislsirikp leidglsiek
      181 lrskanelwd tivkleteky dleerqkrqd ydlkelkerq kqqlrhkalk kgldpealtg
      241 kyppkiqvas kyerrvdtrs yddkkklfeg glteqqkeii ekqwaqqkqq flgrqktklp
      301 kwfgerpgkk pgdpespege edvkaaaede eleepqfdee eeeeeeeeee eeeegekkge
      361 gegeeeeeee eeeeeeeeee eeeeeeeeee
//
LOCUS       XP_076546580             390 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546580
VERSION     XP_076546580.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690465.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="troponin T, skeletal muscle isoform X5"
                     /calculated_mol_wt=46909
     Region          151..>217
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..390
                     /gene="TpnT"
                     /coded_by="XM_076690465.1:124..1296"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqysq perkieesen pefmkrqeqk rsdldeqlke yiaewrkqra keeeelkrlk
       61 ekqakrkitr adeekrlaqk kkeeeerrqr eieekkqrdi eekrkrlees ekkrqammqa
      121 mkdqskkgpn ftitrkdlag nltsaqlern ktkeqleeek kislsirikp leidglsiek
      181 lrskanelwd tivkleteky dleerqkrqd ydlkelkerq kqqlrhkalk kgldpealtg
      241 kyppkiqvas kyerrvdtrs yddkkklfeg glteqqkeii ekqwaqqkqq flgrqktklp
      301 kwfgerpgkk pgdpespege edvkaaaede eleepqfdee eeeeeeeeee eeeegekkge
      361 gegeeeeeee eeeeeeeeee eeeeeeeeee
//
LOCUS       XP_034195388             389 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195388
VERSION     XP_034195388.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339497.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="troponin T, skeletal muscle isoform X6"
                     /calculated_mol_wt=46655
     Region          150..>216
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..389
                     /gene="TpnT"
                     /coded_by="XM_034339497.2:164..1333"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqysg rasqkesenp efmkrqeqkr sdldeqlkey iaewrkqrak eeeelkrlke
       61 kqakrkitra deekrlaqkk keeeerrqre ieekkqrdie ekrkrleese kkrqammqam
      121 kdqskkgpnf titrkdlagn ltsaqlernk tkeqleeekk islsirikpl eidglsiekl
      181 rskanelwdt ivkletekyd leerqkrqdy dlkelkerqk qqlrhkalkk gldpealtgk
      241 yppkiqvask yerrvdtrsy ddkkklfegg lteqqkeiie kqwaqqkqqf lgrqktklpk
      301 wfgerpgkkp gdpespegee dvkaaaedee leepqfdeee eeeeeeeeee eeegekkgeg
      361 egeeeeeeee eeeeeeeeee eeeeeeeee
//
LOCUS       XP_034195385             389 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195385
VERSION     XP_034195385.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339494.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="troponin T, skeletal muscle isoform X6"
                     /calculated_mol_wt=46655
     Region          150..>216
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..389
                     /gene="TpnT"
                     /coded_by="XM_034339494.2:124..1293"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqysg rasqkesenp efmkrqeqkr sdldeqlkey iaewrkqrak eeeelkrlke
       61 kqakrkitra deekrlaqkk keeeerrqre ieekkqrdie ekrkrleese kkrqammqam
      121 kdqskkgpnf titrkdlagn ltsaqlernk tkeqleeekk islsirikpl eidglsiekl
      181 rskanelwdt ivkletekyd leerqkrqdy dlkelkerqk qqlrhkalkk gldpealtgk
      241 yppkiqvask yerrvdtrsy ddkkklfegg lteqqkeiie kqwaqqkqqf lgrqktklpk
      301 wfgerpgkkp gdpespegee dvkaaaedee leepqfdeee eeeeeeeeee eeegekkgeg
      361 egeeeeeeee eeeeeeeeee eeeeeeeee
//
LOCUS       XP_034195389             389 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034195389
VERSION     XP_034195389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="troponin T, skeletal muscle isoform X7"
                     /calculated_mol_wt=46669
     Region          150..>216
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..389
                     /gene="TpnT"
                     /coded_by="XM_034339498.2:164..1333"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqysg rasqkesenp efmkrqeqkr sdldeqlkey iaewrkqrak eeeelkrlke
       61 kqakrkitra deekrlaqkk keeeerrqre ieekkqrdie ekrkrleese kkrqammqam
      121 kdqskkgpnf titrkdlagn ltsaqlernk tkeqleeekk islsirikpl eidglsiekl
      181 rskanelwdt ivkletekyd leerqkrqdy dlkelkerqk qqlrhkalkk gldpealtgk
      241 yppkiqvask yerrvdtrsy ddkkklfegg ydtllaeine klwkqkseqf mkrtktklpk
      301 wfgerpgkkp gdpespegee dvkaaaedee leepqfdeee eeeeeeeeee eeegekkgeg
      361 egeeeeeeee eeeeeeeeee eeeeeeeee
//
LOCUS       XP_034195390             383 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034195390
VERSION     XP_034195390.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339499.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="troponin T, skeletal muscle isoform X8"
                     /calculated_mol_wt=46028
     Region          144..>210
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..383
                     /gene="TpnT"
                     /coded_by="XM_034339499.2:164..1315"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqyse senpefmkrq eqkrsdldeq lkeyiaewrk qrakeeeelk rlkekqakrk
       61 itradeekrl aqkkkeeeer rqreieekkq rdieekrkrl eesekkrqam mqamkdqskk
      121 gpnftitrkd lagnltsaql ernktkeqle eekkislsir ikpleidgls ieklrskane
      181 lwdtivklet ekydleerqk rqdydlkelk erqkqqlrhk alkkgldpea ltgkyppkiq
      241 vaskyerrvd trsyddkkkl fegglteqqk eiiekqwaqq kqqflgrqkt klpkwfgerp
      301 gkkpgdpesp egeedvkaaa edeeleepqf deeeeeeeee eeeeeeegek kgegegeeee
      361 eeeeeeeeee eeeeeeeeee eee
//
LOCUS       XP_034195391             383 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034195391
VERSION     XP_034195391.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339500.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="troponin T, skeletal muscle isoform X8"
                     /calculated_mol_wt=46028
     Region          144..>210
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..383
                     /gene="TpnT"
                     /coded_by="XM_034339500.2:126..1277"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqyse senpefmkrq eqkrsdldeq lkeyiaewrk qrakeeeelk rlkekqakrk
       61 itradeekrl aqkkkeeeer rqreieekkq rdieekrkrl eesekkrqam mqamkdqskk
      121 gpnftitrkd lagnltsaql ernktkeqle eekkislsir ikpleidgls ieklrskane
      181 lwdtivklet ekydleerqk rqdydlkelk erqkqqlrhk alkkgldpea ltgkyppkiq
      241 vaskyerrvd trsyddkkkl fegglteqqk eiiekqwaqq kqqflgrqkt klpkwfgerp
      301 gkkpgdpesp egeedvkaaa edeeleepqf deeeeeeeee eeeeeeegek kgegegeeee
      361 eeeeeeeeee eeeeeeeeee eee
//
LOCUS       XP_034195392             383 aa            linear   INV 03-SEP-2025
DEFINITION  troponin T, skeletal muscle isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034195392
VERSION     XP_034195392.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339501.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="troponin T, skeletal muscle isoform X9"
                     /calculated_mol_wt=46042
     Region          144..>210
                     /region_name="Troponin"
                     /note="pfam00992"
                     /db_xref="CDD:460018"
     CDS             1..383
                     /gene="TpnT"
                     /coded_by="XM_034339501.2:164..1315"
                     /db_xref="GeneID:117611547"
ORIGIN      
        1 msddeeqyse senpefmkrq eqkrsdldeq lkeyiaewrk qrakeeeelk rlkekqakrk
       61 itradeekrl aqkkkeeeer rqreieekkq rdieekrkrl eesekkrqam mqamkdqskk
      121 gpnftitrkd lagnltsaql ernktkeqle eekkislsir ikpleidgls ieklrskane
      181 lwdtivklet ekydleerqk rqdydlkelk erqkqqlrhk alkkgldpea ltgkyppkiq
      241 vaskyerrvd trsyddkkkl feggydtlla eineklwkqk seqfmkrtkt klpkwfgerp
      301 gkkpgdpesp egeedvkaaa edeeleepqf deeeeeeeee eeeeeeegek kgegegeeee
      361 eeeeeeeeee eeeeeeeeee eee
//
LOCUS       XP_034195396             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611548 [Osmia lignaria lignaria].
ACCESSION   XP_034195396
VERSION     XP_034195396.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117611548"
                     /calculated_mol_wt=39442
     Region          18..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,33,37,40,43,50,53)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..341
                     /gene="LOC117611548"
                     /coded_by="XM_034339505.2:238..1263"
                     /db_xref="GeneID:117611548"
ORIGIN      
        1 mdslypnlye rfkseglcpi cllemelapr ytctnghtic yrckpyyhgc ptclapldvq
       61 mppshssssy tpppshflph pvppqfheny ptappaedfl eherswfpse psenqelrsc
      121 ayadsgcgvk vpaqladlhe srcqfrphle eeqlptdlvh qqddlvvcry eavgckvrtt
      181 pwrtsiheky ciykdrheal neindalent sitenqycdp delveckyrk ygcmvnmprk
      241 rkpmhqekcn ykkyandess dneydgsdes sseneydlne qvsckwaeyg crvkpkrcdl
      301 etheemcnhk meecsfkyng cnalftpfkr fahesacqfa g
//
LOCUS       XP_076546581             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611548 [Osmia lignaria lignaria].
ACCESSION   XP_076546581
VERSION     XP_076546581.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690466.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117611548"
                     /calculated_mol_wt=39442
     Region          18..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,33,37,40,43,50,53)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..341
                     /gene="LOC117611548"
                     /coded_by="XM_076690466.1:238..1263"
                     /db_xref="GeneID:117611548"
ORIGIN      
        1 mdslypnlye rfkseglcpi cllemelapr ytctnghtic yrckpyyhgc ptclapldvq
       61 mppshssssy tpppshflph pvppqfheny ptappaedfl eherswfpse psenqelrsc
      121 ayadsgcgvk vpaqladlhe srcqfrphle eeqlptdlvh qqddlvvcry eavgckvrtt
      181 pwrtsiheky ciykdrheal neindalent sitenqycdp delveckyrk ygcmvnmprk
      241 rkpmhqekcn ykkyandess dneydgsdes sseneydlne qvsckwaeyg crvkpkrcdl
      301 etheemcnhk meecsfkyng cnalftpfkr fahesacqfa g
//
LOCUS       XP_076546582             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611548 [Osmia lignaria lignaria].
ACCESSION   XP_076546582
VERSION     XP_076546582.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690467.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117611548"
                     /calculated_mol_wt=39442
     Region          18..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,33,37,40,43,50,53)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..341
                     /gene="LOC117611548"
                     /coded_by="XM_076690467.1:238..1263"
                     /db_xref="GeneID:117611548"
ORIGIN      
        1 mdslypnlye rfkseglcpi cllemelapr ytctnghtic yrckpyyhgc ptclapldvq
       61 mppshssssy tpppshflph pvppqfheny ptappaedfl eherswfpse psenqelrsc
      121 ayadsgcgvk vpaqladlhe srcqfrphle eeqlptdlvh qqddlvvcry eavgckvrtt
      181 pwrtsiheky ciykdrheal neindalent sitenqycdp delveckyrk ygcmvnmprk
      241 rkpmhqekcn ykkyandess dneydgsdes sseneydlne qvsckwaeyg crvkpkrcdl
      301 etheemcnhk meecsfkyng cnalftpfkr fahesacqfa g
//
LOCUS       XP_076546583             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611548 [Osmia lignaria lignaria].
ACCESSION   XP_076546583
VERSION     XP_076546583.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690468.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117611548"
                     /calculated_mol_wt=39442
     Region          18..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,33,37,40,43,50,53)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..341
                     /gene="LOC117611548"
                     /coded_by="XM_076690468.1:238..1263"
                     /db_xref="GeneID:117611548"
ORIGIN      
        1 mdslypnlye rfkseglcpi cllemelapr ytctnghtic yrckpyyhgc ptclapldvq
       61 mppshssssy tpppshflph pvppqfheny ptappaedfl eherswfpse psenqelrsc
      121 ayadsgcgvk vpaqladlhe srcqfrphle eeqlptdlvh qqddlvvcry eavgckvrtt
      181 pwrtsiheky ciykdrheal neindalent sitenqycdp delveckyrk ygcmvnmprk
      241 rkpmhqekcn ykkyandess dneydgsdes sseneydlne qvsckwaeyg crvkpkrcdl
      301 etheemcnhk meecsfkyng cnalftpfkr fahesacqfa g
//
LOCUS       XP_076546584             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611548 [Osmia lignaria lignaria].
ACCESSION   XP_076546584
VERSION     XP_076546584.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690469.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117611548"
                     /calculated_mol_wt=39442
     Region          18..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,33,37,40,43,50,53)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..341
                     /gene="LOC117611548"
                     /coded_by="XM_076690469.1:238..1263"
                     /db_xref="GeneID:117611548"
ORIGIN      
        1 mdslypnlye rfkseglcpi cllemelapr ytctnghtic yrckpyyhgc ptclapldvq
       61 mppshssssy tpppshflph pvppqfheny ptappaedfl eherswfpse psenqelrsc
      121 ayadsgcgvk vpaqladlhe srcqfrphle eeqlptdlvh qqddlvvcry eavgckvrtt
      181 pwrtsiheky ciykdrheal neindalent sitenqycdp delveckyrk ygcmvnmprk
      241 rkpmhqekcn ykkyandess dneydgsdes sseneydlne qvsckwaeyg crvkpkrcdl
      301 etheemcnhk meecsfkyng cnalftpfkr fahesacqfa g
//
LOCUS       XP_034195397             341 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611548 [Osmia lignaria lignaria].
ACCESSION   XP_034195397
VERSION     XP_034195397.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339506.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="uncharacterized protein LOC117611548"
                     /calculated_mol_wt=39442
     Region          18..56
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(18,21,33,37,40,43,50,53)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     CDS             1..341
                     /gene="LOC117611548"
                     /coded_by="XM_034339506.2:393..1418"
                     /db_xref="GeneID:117611548"
ORIGIN      
        1 mdslypnlye rfkseglcpi cllemelapr ytctnghtic yrckpyyhgc ptclapldvq
       61 mppshssssy tpppshflph pvppqfheny ptappaedfl eherswfpse psenqelrsc
      121 ayadsgcgvk vpaqladlhe srcqfrphle eeqlptdlvh qqddlvvcry eavgckvrtt
      181 pwrtsiheky ciykdrheal neindalent sitenqycdp delveckyrk ygcmvnmprk
      241 rkpmhqekcn ykkyandess dneydgsdes sseneydlne qvsckwaeyg crvkpkrcdl
      301 etheemcnhk meecsfkyng cnalftpfkr fahesacqfa g
//
LOCUS       XP_034195398             254 aa            linear   INV 03-SEP-2025
DEFINITION  trypsin-1-like [Osmia lignaria lignaria].
ACCESSION   XP_034195398
VERSION     XP_034195398.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339507.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195398.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="trypsin-1-like"
                     /calculated_mol_wt=27392
     Region          26..252
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            26
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(68,114,210)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(204,225,227)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..254
                     /gene="LOC143305205"
                     /coded_by="XM_034339507.2:105..869"
                     /db_xref="GeneID:143305205"
ORIGIN      
        1 mllttlsvls llslsnagll rfdprivnge dakvgeipyq vslqnqgssf hfcggsvlne
       61 nyvitaahcv egktaagikv iagtinltap ksehnvkkii iheqynpnds wkndiallkv
      121 ekpfvksaqi afvplpqknq gvkandnavv sgwgrlwkgg pttvhlqrvn iliadqeyck
      181 ytyknianiy nshvcaydps vakgscngds ggpltvngvl tglvswamgc artdyptvyt
      241 rvsahldwik shav
//
LOCUS       XP_034195399             254 aa            linear   INV 03-SEP-2025
DEFINITION  chymotrypsin-2-like [Osmia lignaria lignaria].
ACCESSION   XP_034195399
VERSION     XP_034195399.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339508.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="chymotrypsin-2-like"
                     /calculated_mol_wt=27572
     Region          26..252
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            26
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(68,114,210)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(204,225,227)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..254
                     /gene="LOC117611549"
                     /coded_by="XM_034339508.2:104..868"
                     /db_xref="GeneID:117611549"
ORIGIN      
        1 mllttlslft llslsnagvl rfdprivnge dakvgeipyq vslqnkdnsf hfcggsvlne
       61 nyvitaahcv igktaasikv iagtinlaaq kseynvknii ihekynpnds wkndiallkv
      121 ekpfvkseqi afvplppkdq vvkandkavv sgwgrlsqgg pttvhlqrvn imiadqeyck
      181 ysykgsqnvy dsqvcaydpr vgkgschgds ggpltvngrl tglvswsrgc adtyspavft
      241 rvsayiewik snav
//
LOCUS       XP_034195401             254 aa            linear   INV 03-SEP-2025
DEFINITION  chymotrypsin-2-like [Osmia lignaria lignaria].
ACCESSION   XP_034195401
VERSION     XP_034195401.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339510.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195401.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="chymotrypsin-2-like"
                     /calculated_mol_wt=27733
     Region          26..252
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            26
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(68,114,210)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(204,225,227)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..254
                     /gene="LOC117611550"
                     /coded_by="XM_034339510.2:76..840"
                     /db_xref="GeneID:117611550"
ORIGIN      
        1 militvslft llslsnagll rfdprivnge dakegefpyq vslqnkdnsf hfcggsvlne
       61 nyvitaahcv egktaasikv iagtinlaaq kseynvkkii ihekydeads wkndiallkv
      121 dksfvksqqv afvplppknq vvkandkavv sgwgrlwqgg pttvhlqrvn imiadqeyck
      181 ytykgsqniy dsqicaydps vgkgpchgds ggplivngrl tglvswlrgc gntyspavft
      241 rvsayiewik snav
//
LOCUS       XP_034195402             254 aa            linear   INV 03-SEP-2025
DEFINITION  chymotrypsin-2-like [Osmia lignaria lignaria].
ACCESSION   XP_034195402
VERSION     XP_034195402.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339511.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="chymotrypsin-2-like"
                     /calculated_mol_wt=27869
     Region          26..252
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            26
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(68,114,210)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(204,225,227)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..254
                     /gene="LOC117611551"
                     /coded_by="XM_034339511.2:105..869"
                     /db_xref="GeneID:117611551"
ORIGIN      
        1 mllttlslft llslsnagll rfdprivnge dakegeipyq vslqnkdssf hfcggsvlne
       61 nyvitaahcv egktapsikv iagtinltap ksehnvkkii iherynktds wkndiallkv
      121 ekpfvksqqi afvplppknq vvkandkavv sgwgrlwldg pkpvhlqrat iliadqefck
      181 ytykgsqniy dsqvcaydpt vkkgackgds ggpltvngrl tglvswsrgc arteypsvyt
      241 rvsahidwik shav
//
LOCUS       XP_034195404             254 aa            linear   INV 03-SEP-2025
DEFINITION  chymotrypsin-2-like [Osmia lignaria lignaria].
ACCESSION   XP_034195404
VERSION     XP_034195404.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339513.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195404.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="chymotrypsin-2-like"
                     /calculated_mol_wt=27688
     Region          26..252
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            26
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(68,114,210)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(204,225,227)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..254
                     /gene="LOC143305229"
                     /coded_by="XM_034339513.2:109..873"
                     /db_xref="GeneID:143305229"
ORIGIN      
        1 mllttlsift llslsnagll rfdprivnge dakegeipyq vslqnkdssf hfcggsvlne
       61 nyvitaahcv esktavgikv iagtinlttp ksehnvkkii ihekynpnds wkndiallkv
      121 ekpfvkseqi afvplppknq vvkandkavv sgwgrlwhgg pttvhlqrvn imiadqeyck
      181 niykgsqnvy dsqvcayesg vekgpchgds ggpltvngrl tglaswlrsc gntssptvft
      241 rvsayiewik snav
//
LOCUS       XP_076546585             185 aa            linear   INV 03-SEP-2025
DEFINITION  U11/U12 small nuclear ribonucleoprotein 35 kDa protein isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_076546585
VERSION     XP_076546585.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690470.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..185
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..185
                     /product="U11/U12 small nuclear ribonucleoprotein 35 kDa
                     protein isoform X1"
                     /calculated_mol_wt=20878
     Region          59..150
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..185
                     /gene="LOC117611553"
                     /coded_by="XM_076690470.1:16..573"
                     /db_xref="GeneID:117611553"
ORIGIN      
        1 mtssilitda wqnwspyake ydplkagsid gtdtephdka ishameahye pprglkskpe
       61 rtlfvarfgp kvtrhdlkef fcrygdvisa kvivdivtgl sqgygfvemr tedearrair
      121 rsadamlkgh kifvdyecdr tmkgwkprrl gggfggkkes gqlrfggrdr pfkkpivpni
      181 irphn
//
LOCUS       XP_034195408             179 aa            linear   INV 03-SEP-2025
DEFINITION  U11/U12 small nuclear ribonucleoprotein 35 kDa protein isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034195408
VERSION     XP_034195408.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339517.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..179
                     /product="U11/U12 small nuclear ribonucleoprotein 35 kDa
                     protein isoform X2"
                     /calculated_mol_wt=20263
     Region          53..144
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..179
                     /gene="LOC117611553"
                     /coded_by="XM_034339517.2:1438..1977"
                     /db_xref="GeneID:117611553"
ORIGIN      
        1 mtdawqnwsp yakeydplka gsidgtdtep hdkaishame ahyepprglk skpertlfva
       61 rfgpkvtrhd lkeffcrygd visakvivdi vtglsqgygf vemrtedear rairrsadam
      121 lkghkifvdy ecdrtmkgwk prrlgggfgg kkesgqlrfg grdrpfkkpi vpniirphn
//
LOCUS       XP_034195409             183 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611554 [Osmia lignaria lignaria].
ACCESSION   XP_034195409
VERSION     XP_034195409.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339518.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..183
                     /product="uncharacterized protein LOC117611554"
                     /calculated_mol_wt=20390
     CDS             1..183
                     /gene="LOC117611554"
                     /coded_by="XM_034339518.2:1..552"
                     /db_xref="GeneID:117611554"
ORIGIN      
        1 mlrtrvilpv lifalcltfy ctacspdigq sldlkqfage wffaagtpin eslsrcgrfl
       61 vrqtspstfa ikftavsykn nipisfnvdg stngnkiigt wqlqgskril gpfqhvivea
      121 kyksvvgmlv csdngsshqq rykfsmiwsr erslplpvfk eiksklgvyi nqgeirmvdh
      181 gnc
//
LOCUS       XP_076546586              66 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305726 [Osmia lignaria lignaria].
ACCESSION   XP_076546586
VERSION     XP_076546586.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690471.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..66
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..66
                     /product="uncharacterized protein LOC143305726"
                     /calculated_mol_wt=7377
     CDS             1..66
                     /gene="LOC143305726"
                     /coded_by="XM_076690471.1:3542..3742"
                     /db_xref="GeneID:143305726"
ORIGIN      
        1 mfieclpwfs wlscntsspe ptkvyglsfk llglspelgq rvhfaegtnl rcredkkpsd
       61 eseesv
//
LOCUS       XP_034191620             332 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609421 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191620
VERSION     XP_034191620.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191620.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..332
                     /product="uncharacterized protein LOC117609421 isoform X1"
                     /calculated_mol_wt=36593
     Region          3..71
                     /region_name="DSRM_PRKRA-like_rpt1"
                     /note="first double-stranded RNA binding motif of protein
                     activator of the interferon-induced protein kinase (PRKRA)
                     and similar proteins; cd19862"
                     /db_xref="CDD:380691"
     Site            order(3..4,6..7,10..11,14..15,22,30..32,34,36,52..55,58)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380691"
     Region          109..175
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Site            order(115..116,118,142,159..161,164)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380679"
     CDS             1..332
                     /gene="LOC117609421"
                     /coded_by="XM_034335729.2:567..1565"
                     /db_xref="GeneID:117609421"
ORIGIN      
        1 mnktpvsilq emmvkkhtvp nyelihdggg thmntftyqv tcdglsatgt grskkdakhe
       61 aakamletia ahrgypqlpa spaqspvrtp lpqpvpevpr ippnvpfvna igalqdlcve
      121 nnlqepqytq isdvgpphak iftiqcgvst fkevgvartk kqakqeaakk mlekisdlvs
      181 sieddindtt nevksnalak adylllsrlp sskkvnlgik iaeyhtklks sftstvcddl
      241 iqtleslipe dyndvseefv edllskfsdf lltlnlqiev kimksnvpdk hvvimkidss
      301 ppisqvscen tmtqarftai qelinimiff lk
//
LOCUS       XP_076546587             332 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609421 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546587
VERSION     XP_076546587.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690472.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..332
                     /product="uncharacterized protein LOC117609421 isoform X1"
                     /calculated_mol_wt=36593
     Region          3..71
                     /region_name="DSRM_PRKRA-like_rpt1"
                     /note="first double-stranded RNA binding motif of protein
                     activator of the interferon-induced protein kinase (PRKRA)
                     and similar proteins; cd19862"
                     /db_xref="CDD:380691"
     Site            order(3..4,6..7,10..11,14..15,22,30..32,34,36,52..55,58)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380691"
     Region          109..175
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Site            order(115..116,118,142,159..161,164)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380679"
     CDS             1..332
                     /gene="LOC117609421"
                     /coded_by="XM_076690472.1:464..1462"
                     /db_xref="GeneID:117609421"
ORIGIN      
        1 mnktpvsilq emmvkkhtvp nyelihdggg thmntftyqv tcdglsatgt grskkdakhe
       61 aakamletia ahrgypqlpa spaqspvrtp lpqpvpevpr ippnvpfvna igalqdlcve
      121 nnlqepqytq isdvgpphak iftiqcgvst fkevgvartk kqakqeaakk mlekisdlvs
      181 sieddindtt nevksnalak adylllsrlp sskkvnlgik iaeyhtklks sftstvcddl
      241 iqtleslipe dyndvseefv edllskfsdf lltlnlqiev kimksnvpdk hvvimkidss
      301 ppisqvscen tmtqarftai qelinimiff lk
//
LOCUS       XP_076546588             330 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609421 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546588
VERSION     XP_076546588.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690473.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="uncharacterized protein LOC117609421 isoform X2"
                     /calculated_mol_wt=36509
     Region          9..69
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Site            order(9,11,34,51..53,56)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380679"
     Region          107..173
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Site            order(113..114,116,140,157..159,162)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380679"
     CDS             1..330
                     /gene="LOC117609421"
                     /coded_by="XM_076690473.1:307..1299"
                     /db_xref="GeneID:117609421"
ORIGIN      
        1 myirmfegem mvkkhtvpny elihdgggth mntftyqvtc dglsatgtgr skkdakheaa
       61 kamletiaah rgypqlpasp aqspvrtplp qpvpevprip pnvpfvnaig alqdlcvenn
      121 lqepqytqis dvgpphakif tiqcgvstfk evgvartkkq akqeaakkml ekisdlvssi
      181 eddindttne vksnalakad ylllsrlpss kkvnlgikia eyhtklkssf tstvcddliq
      241 tleslipedy ndvseefved llskfsdfll tlnlqievki mksnvpdkhv vimkidsspp
      301 isqvscentm tqarftaiqe linimifflk
//
LOCUS       XP_034191631             321 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609421 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191631
VERSION     XP_034191631.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335740.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191631.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="uncharacterized protein LOC117609421 isoform X3"
                     /calculated_mol_wt=35352
     Region          3..60
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Region          98..164
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Site            order(104..105,107,131,148..150,153)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380679"
     CDS             1..321
                     /gene="LOC117609421"
                     /coded_by="XM_034335740.2:435..1400"
                     /db_xref="GeneID:117609421"
ORIGIN      
        1 mmvkkhtvpn yelihdgggt hmntftyqvt cdglsatgtg rskkdakhea akamletiaa
       61 hrgypqlpas paqspvrtpl pqpvpevpri ppnvpfvnai galqdlcven nlqepqytqi
      121 sdvgpphaki ftiqcgvstf kevgvartkk qakqeaakkm lekisdlvss ieddindttn
      181 evksnalaka dylllsrlps skkvnlgiki aeyhtklkss ftstvcddli qtlesliped
      241 yndvseefve dllskfsdfl ltlnlqievk imksnvpdkh vvimkidssp pisqvscent
      301 mtqarftaiq elinimiffl k
//
LOCUS       XP_034191713            4665 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal heavy chain 1 [Osmia lignaria lignaria].
ACCESSION   XP_034191713
VERSION     XP_034191713.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335822.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191713.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4665
                     /product="dynein axonemal heavy chain 1"
                     /calculated_mol_wt=537604
     Region          275..834
                     /region_name="DHC_N1"
                     /note="Dynein heavy chain, N-terminal region 1; pfam08385"
                     /db_xref="CDD:462457"
     Region          1158..4313
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1431..1836
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          1971..2298
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          4366..4662
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..4665
                     /gene="Dhc1"
                     /coded_by="XM_034335822.2:330..14327"
                     /db_xref="GeneID:117609454"
ORIGIN      
        1 mfvqapalmi qqksenseeg sdeessgdvl qkrreecerk arrgemdprl efafqllmda
       61 tqlsrhevmd hvfegdmlde inllflphmr stlvwyyqem depetlrpve itqtrsssar
      121 ggpnvltpkv keqketkepg kkklfltdgw qvpctgiciy mfrlnvskql peegfqkdly
      181 tgvidtkkvg iitaiqrvve yffmdalahp gsegeddcpm ikslllpglr sfcsalkvce
      241 qvahegrifn dgetlmnevh nfeeakafia kenavnivee rvkawikrlk dfmaeskqvk
      301 rendtsgpqq eleywkrrga qfsqlvnklq dheirmsllc lqvarsklik dwvdtedeit
      361 ycyneakdna kfiqalekcc hclylddpvk mrdslvsllq tvrliysvsr yyntsertss
      421 lmikitnqmi vacrdyvtnr gretvwgqdr aaarsklkhc lrlnkayret yrlvrcspai
      481 teqefsfset hvfgrfdlfc drirkiltmf eliqdyknlf errmeglllg ealddairif
      541 eeaeniattk sydyldprnt efdtdydsfm aktdslkqni gniieknyed vwetpqgirf
      601 ltrfekvsek ipltkldeky nivvkycdke vdrilrlfkk qrddpplprh mpaiagrltw
      661 anslkvhlde latsvsthpv lktlpltmel ekrynyalgi lnqyksdiae vwthqnswvi
      721 edclkrsllt vdektgtikv nlegrimlli readclskln leipivalti ltkkdhftli
      781 tdalqlmiek fvfttkrvkl evrplllphl vrvasllepg lmsltwtdpe wknfyqntke
      841 qikefdilit rvhdvydnri lqvltsmqki tlhslpesdq pwtieefvek teeicrlaav
      901 dlyrksmmve eaveevldlv knaaenfksa anssqfdffn ieedeetvdq taqqdwslvw
      961 slfddphqll atpgslpkgm qdmvrnavse mrryysrkvv dvlikvtrss ldairkrfvr
     1021 eidseetpsp ifllhatlmi pvvtvkpsld dvqeammlvg ktiagvakgv sqwnsrvnkn
     1081 swkqaypkrt fadivmraqi qaakkkreep edarirrrkl ykiiseerpm fpvqesnfhg
     1141 mlmdnkevmk llsmlntcmq efkpdlvefi drwkpykflw kneksirell qislpefest
     1201 lrkhseledl latepdmiif gtsiavstek lkyglyteik acthkigqam kkkyrremey
     1261 vyalmnemdr kldrqirdld dvrlimdtlk kireqevdme lridpieeaf nivtryevpv
     1321 dqecleqvdn lrytwqqlln raqqshvlll elqpqfeedl rnnlerfrid ntmyceeyrs
     1381 sgpmqpglsp reasdrqilf qnrfdgmwrk lqtyqsgeel fglpitdype lsqirkelnl
     1441 lqklyklynd vidrvnsyyd ipwgevnied innelmefqn rcrklpkglk ewpaffalkk
     1501 tiddfndmcp llelmankam kprhwhrive vtgysfdlds egfclknile apllkykedi
     1561 edicisamke kdieaklrtv vnewsshelt fmtfnnrgel llrgdttaet igqledslmv
     1621 lgslmsnryn apfrkqiqqw ladlsntnei lerwllvqnm wvyleavfvg gdiakqlpke
     1681 akrfskidks wqkimqrahe tpgvvpccvg ddllkqllph lqeqlelcqk slsgylekkr
     1741 mmfprfffvs dpalleilgq asdshtiqnh llsifdntry vkfhdieynk mmaiissege
     1801 tillekavra egsvetwltq llqtsqqslh siirqcysmi ndinfnllgf lnkmpaqigl
     1861 lgiqmiwtrd seqalaqara dkkimgetnn rfldllntli dqttrdlaki ertkfetlit
     1921 ihvhqrdifd ilcrlnvrsv ndfewlkqcr fyfkedldkt sicitdvnft yqneylgcte
     1981 rlvitpltdr cyitlaqals msmggspcgp agtgktetvk dmgktlakyv vvfncsdqmd
     2041 yrglgriykg laqsgswgcf defnrielpv lsvaaqqvav vlaakkekkk qfvftdgdli
     2101 dmcpelgifi tmnpgyagrk elpenlkiqf rtvammvpdr qiiirvklas cgflenitla
     2161 rkfytlyklc eeqltkqvhy dfglrnilsv lrtlgaskrv nskdsestiv mrvlrdmnls
     2221 klidedeplf islvadlfpn qalektsype leaainqqve eagliyhppw vlkliqlyet
     2281 qrvrhgimtl gptgagkttc ihilmkaltq cgnfhremrm npksitaaqm fgrldvatnd
     2341 wtdgifsalw rktlklkege hvwlvldgpv dsiwienlns vlddnktltl angdrlpmaa
     2401 tckiifephn idnaspatvs rngmvymsss gldwnpvvta wlktrstleq evfgqcfads
     2461 faqiyswstq vltltidvlq cnivkqmlcl leglvplets ieeeedeeme eekrqpmtme
     2521 hlqrfyvftq iwgigallet sdrakydqyl renfdnldlp ntennpeakl fdfyvtekgk
     2581 wdtwtsmvtn yvypeystpd ysnvlvpipd nvriqylidl igrqdkavll igeqgsaktv
     2641 mmksymkkan pettlsrsfn fssatspfqf qktiesyvek rlgntfgppg gkkmmvfvdd
     2701 inlpqinewg dqvtneivrq tmdmkgfysl ekpgdftniv dmtylaamcq pgggrndips
     2761 rlkrqfcifn ctlpdkasid rifsvlgegh ynnkrgfsvd vrnlvkkmvp ltrilwertr
     2821 shllptpakf hyvfnlrdls riwqgmvgtl stvidkenvl mllwkhecsr vfsdrftiqt
     2881 dkdwfegelv rvvnemlgek yvdmldqdpa fvdfmrdape ptgeegedad velpkvyepv
     2941 yddqvlrdrl emflsqfnem qrgsgmdlvf fpdamlhlvk isrvirhpkg nvmlvgvggs
     3001 gkqsltklss fiagyktfqi tltrsynvan fledlrylyr tcgaqgkgtt fiftdldike
     3061 egfleylnni lssgvisnlf trdeqqeiil eltpilrren pkrsinnelv mdfflqrtcq
     3121 nlhvvfcfsp vgekfrnraq rfpalisgct idwfqpwpkd alilvakhfl hdfsiactse
     3181 vkdelvhalg siqdivslts aeyfqrfrra thvtpksyln figgykniyq skqhelgega
     3241 krmdtglakl eeasisveil krdlavmekd lvqasekaet vlqevteram qaeafknqvq
     3301 kvkekaeqlv aciaeekala eqkleaakpa leeaeaalnt ikpahiatvr klgrpphlim
     3361 rimdcvlilf qrkigpvvpd taapcpkpsw seslkmmast tfllqlqnyp kdiinnemve
     3421 llqpyfkmed ynmetarrvc gdvagllswt kamaffhsvn kevlplkanl alqearlkva
     3481 medlanaere lseremalqa vkeqydsavs ekqrlteaan vcirkmtaat alinglggek
     3541 irwteqssef kvqlgrlvgd vllatgflsy cgpynqqyra slvtswmkil atkaipfttn
     3601 lnitnmlvds atmsewtlqg lpndelsvqn alivtksssy pllvdpqnqg kmwiknkecm
     3661 nelqitslnh kyfrthleds lslgrpllie diaeeldpvl dnvleknfik sgsiekvivg
     3721 dkecdvmpgf mlyvttklpn payspeisak tsiidftvtm lgledqllgr vilmekadle
     3781 aervalfesv mtnqrsmkel estllhrlts segslvddea linvlretkv taesvneklk
     3841 vsaltekkit lareefrava srgsilyfli vemsnvnvmy qnslrqflti fdnsitkstk
     3901 spitserini ilrhltyevw aftlrslyer hkslftlmla mkidchrgai thaefnafik
     3961 ggasldlnav vqkpfrwild itwlnlveis kletfsdilm kieynekewr vwyekekpee
     4021 eelpcgyqkk ldvfrkllli rswspdrtls qarkyviesl gkeygeakil dleatwlese
     4081 vrtplicils igsdpspqit ilakqksiql ksvsmgqgqe fharrlisda mntgswvllq
     4141 nihlslpfct evmdalvete tvheafrlwm ttevhpqfpi gllqmaikft neppqgiras
     4201 lkrtyqgvtq dtldystqsq wppllyavaf lhtvvqerrk fgplgwnipy efnqadfaas
     4261 vqfiqnhldd mdpkrgvswp tvcymlgevq yggrvtddfd krllttfthv wfcdvllrsg
     4321 fefyrgykvp qtknlqgyld yidslpatdt pevfglhpna dityqintak gildtilsvq
     4381 pkeggaqgge trenvvykla sdmleklpkq ynsfevkeal qrmgallpmn iflrqeidri
     4441 trvikevrtt ltdlklaieg tivmsqglrk sldamydari pekwlkiswe satlgfwyte
     4501 llerdyqfrq wcihgrpkvf wmtgffnpqg fltamrqevt rahkgwalds vvlqnlitrf
     4561 skeeiknqpq egvyvhglfl egasldrkts klveskpkvl yeqmpviyiy ainttagkdp
     4621 klyecpiyrk pqrtdskyig sidfetdhnp rhwtlrgval lcdik
//
LOCUS       XP_034195217             733 aa            linear   INV 03-SEP-2025
DEFINITION  cysteine--tRNA ligase, cytoplasmic isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195217
VERSION     XP_034195217.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339326.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..733
                     /product="cysteine--tRNA ligase, cytoplasmic isoform X1"
                     /calculated_mol_wt=84342
     Region          3..728
                     /region_name="PTZ00399"
                     /note="cysteinyl-tRNA-synthetase; Provisional"
                     /db_xref="CDD:240402"
     CDS             1..733
                     /gene="CysRS"
                     /coded_by="XM_034339326.2:225..2426"
                     /db_xref="GeneID:117611390"
ORIGIN      
        1 makrlqppwc ppqrknaatl kvynsltrdk evfipqfgnr ilwyscgptv ydashmghar
       61 syisfdilrr vlsdyfgydi lyvmnitdid dkiikrarqn ylyekyveed hpldeildda
      121 ksvmctfedt vkatndvdkk cmlqkmlcdv ekaienlena vklkdnnkik eyqelllkea
      181 rdplaewldk kngatvtehs ifsklsqhwe sefhkdmdal nvlkpnvltr vseyipeiia
      241 fiqkiiengv ayesngsiyf dvasfdkqdk hyyaklvpea ygdtssleeg egvlsstevs
      301 ekrsstdfal wkcskegepw wdspwgkgrp gwhiecsvma siicgesldi htggvdlkfp
      361 hhdneiaqae ayfnnsnwvr yflhaghlti agckmskslk nfitiqdalk khsarqlrla
      421 fllhswkdtl dysdntmnma mqyekflnef flnvkcgirs lgsetnintf tkwtnseiel
      481 nkkfsnakda vhaalcdnid trsaldaire lvthcniyrk qvkhqntlll rdiavyitkm
      541 ftifgaipyp hdtigfpvds itensnveet vmpylevlas frekvrnhak vlkandilee
      601 cdklrdeilp nigvrledsn eelckvklvn keellrekec krkmemekll ekekrkaeat
      661 aaaaakeaqr kippsemfkl ekdkysqfds ngipthdisg keiskgqmkk lqklqqaqek
      721 ryneyfasiq ngs
//
LOCUS       XP_034195220             733 aa            linear   INV 03-SEP-2025
DEFINITION  cysteine--tRNA ligase, cytoplasmic isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195220
VERSION     XP_034195220.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339329.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..733
                     /product="cysteine--tRNA ligase, cytoplasmic isoform X1"
                     /calculated_mol_wt=84342
     Region          3..728
                     /region_name="PTZ00399"
                     /note="cysteinyl-tRNA-synthetase; Provisional"
                     /db_xref="CDD:240402"
     CDS             1..733
                     /gene="CysRS"
                     /coded_by="XM_034339329.2:147..2348"
                     /db_xref="GeneID:117611390"
ORIGIN      
        1 makrlqppwc ppqrknaatl kvynsltrdk evfipqfgnr ilwyscgptv ydashmghar
       61 syisfdilrr vlsdyfgydi lyvmnitdid dkiikrarqn ylyekyveed hpldeildda
      121 ksvmctfedt vkatndvdkk cmlqkmlcdv ekaienlena vklkdnnkik eyqelllkea
      181 rdplaewldk kngatvtehs ifsklsqhwe sefhkdmdal nvlkpnvltr vseyipeiia
      241 fiqkiiengv ayesngsiyf dvasfdkqdk hyyaklvpea ygdtssleeg egvlsstevs
      301 ekrsstdfal wkcskegepw wdspwgkgrp gwhiecsvma siicgesldi htggvdlkfp
      361 hhdneiaqae ayfnnsnwvr yflhaghlti agckmskslk nfitiqdalk khsarqlrla
      421 fllhswkdtl dysdntmnma mqyekflnef flnvkcgirs lgsetnintf tkwtnseiel
      481 nkkfsnakda vhaalcdnid trsaldaire lvthcniyrk qvkhqntlll rdiavyitkm
      541 ftifgaipyp hdtigfpvds itensnveet vmpylevlas frekvrnhak vlkandilee
      601 cdklrdeilp nigvrledsn eelckvklvn keellrekec krkmemekll ekekrkaeat
      661 aaaaakeaqr kippsemfkl ekdkysqfds ngipthdisg keiskgqmkk lqklqqaqek
      721 ryneyfasiq ngs
//
LOCUS       XP_034195224             733 aa            linear   INV 03-SEP-2025
DEFINITION  cysteine--tRNA ligase, cytoplasmic isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195224
VERSION     XP_034195224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339333.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..733
                     /product="cysteine--tRNA ligase, cytoplasmic isoform X1"
                     /calculated_mol_wt=84342
     Region          3..728
                     /region_name="PTZ00399"
                     /note="cysteinyl-tRNA-synthetase; Provisional"
                     /db_xref="CDD:240402"
     CDS             1..733
                     /gene="CysRS"
                     /coded_by="XM_034339333.2:26..2227"
                     /db_xref="GeneID:117611390"
ORIGIN      
        1 makrlqppwc ppqrknaatl kvynsltrdk evfipqfgnr ilwyscgptv ydashmghar
       61 syisfdilrr vlsdyfgydi lyvmnitdid dkiikrarqn ylyekyveed hpldeildda
      121 ksvmctfedt vkatndvdkk cmlqkmlcdv ekaienlena vklkdnnkik eyqelllkea
      181 rdplaewldk kngatvtehs ifsklsqhwe sefhkdmdal nvlkpnvltr vseyipeiia
      241 fiqkiiengv ayesngsiyf dvasfdkqdk hyyaklvpea ygdtssleeg egvlsstevs
      301 ekrsstdfal wkcskegepw wdspwgkgrp gwhiecsvma siicgesldi htggvdlkfp
      361 hhdneiaqae ayfnnsnwvr yflhaghlti agckmskslk nfitiqdalk khsarqlrla
      421 fllhswkdtl dysdntmnma mqyekflnef flnvkcgirs lgsetnintf tkwtnseiel
      481 nkkfsnakda vhaalcdnid trsaldaire lvthcniyrk qvkhqntlll rdiavyitkm
      541 ftifgaipyp hdtigfpvds itensnveet vmpylevlas frekvrnhak vlkandilee
      601 cdklrdeilp nigvrledsn eelckvklvn keellrekec krkmemekll ekekrkaeat
      661 aaaaakeaqr kippsemfkl ekdkysqfds ngipthdisg keiskgqmkk lqklqqaqek
      721 ryneyfasiq ngs
//
LOCUS       XP_076546589             653 aa            linear   INV 03-SEP-2025
DEFINITION  cysteine--tRNA ligase, cytoplasmic isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546589
VERSION     XP_076546589.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690474.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..653
                     /product="cysteine--tRNA ligase, cytoplasmic isoform X2"
                     /calculated_mol_wt=75120
     Region          112..648
                     /region_name="PTZ00399"
                     /note="cysteinyl-tRNA-synthetase; Provisional"
                     /db_xref="CDD:240402"
     CDS             1..653
                     /gene="CysRS"
                     /coded_by="XM_076690474.1:151..2112"
                     /db_xref="GeneID:117611390"
ORIGIN      
        1 mmhhiwdmld dkiikrarqn ylyekyveed hpldeildda ksvmctfedt vkatndvdkk
       61 cmlqkmlcdv ekaienlena vklkdnnkik eyqelllkea rdplaewldk kngatvtehs
      121 ifsklsqhwe sefhkdmdal nvlkpnvltr vseyipeiia fiqkiiengv ayesngsiyf
      181 dvasfdkqdk hyyaklvpea ygdtssleeg egvlsstevs ekrsstdfal wkcskegepw
      241 wdspwgkgrp gwhiecsvma siicgesldi htggvdlkfp hhdneiaqae ayfnnsnwvr
      301 yflhaghlti agckmskslk nfitiqdalk khsarqlrla fllhswkdtl dysdntmnma
      361 mqyekflnef flnvkcgirs lgsetnintf tkwtnseiel nkkfsnakda vhaalcdnid
      421 trsaldaire lvthcniyrk qvkhqntlll rdiavyitkm ftifgaipyp hdtigfpvds
      481 itensnveet vmpylevlas frekvrnhak vlkandilee cdklrdeilp nigvrledsn
      541 eelckvklvn keellrekec krkmemekll ekekrkaeat aaaaakeaqr kippsemfkl
      601 ekdkysqfds ngipthdisg keiskgqmkk lqklqqaqek ryneyfasiq ngs
//
LOCUS       XP_034195235             307 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger protein 146 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195235
VERSION     XP_034195235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339344.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="ring finger protein 146 isoform X1"
                     /calculated_mol_wt=35038
     Region          33..82
                     /region_name="RING-HC_RNF146"
                     /note="RING finger, HC subclass, found in RING finger
                     protein 146 (RNF146) and similar proteins; cd16546"
                     /db_xref="CDD:438208"
     Site            order(36..37,39,68,70..71)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438208"
     Region          103..194
                     /region_name="WWE"
                     /note="Domain in Deltex and TRIP12 homologues. Possibly
                     involved in regulation of ubiquitin-mediated proteolysis;
                     smart00678"
                     /db_xref="CDD:128922"
     CDS             1..307
                     /gene="Rnf146"
                     /coded_by="XM_034339344.2:205..1128"
                     /db_xref="GeneID:117611404"
ORIGIN      
        1 maqaklnsqe etsgtlndke kesdekegtt tvlecavclq pciyparlpc nhiycylcvk
       61 gvanqskrcp mcrqeippdf lnrpqlvevd eaqkesehfe eeyqwfyegr ngwwkydsrt
      121 sqdlediynl gwwqydqrts heletaykqg krncelliag flyiadfgsm lqlrrndpsr
      181 rrkikrdlyn vpkkgvaglr lnhqdeeiir evrgaerpas pasddmgimf stgdgtntpv
      241 ppsntpqtpa ggtasgdatp lndrmeqrds lhqvleqmrs lvlrehlssd tddeldedee
      301 tsthptl
//
LOCUS       XP_034195241             303 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger protein 146 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195241
VERSION     XP_034195241.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339350.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="ring finger protein 146 isoform X2"
                     /calculated_mol_wt=34559
     Region          33..82
                     /region_name="RING-HC_RNF146"
                     /note="RING finger, HC subclass, found in RING finger
                     protein 146 (RNF146) and similar proteins; cd16546"
                     /db_xref="CDD:438208"
     Site            order(36..37,39,68,70..71)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438208"
     Region          103..194
                     /region_name="WWE"
                     /note="Domain in Deltex and TRIP12 homologues. Possibly
                     involved in regulation of ubiquitin-mediated proteolysis;
                     smart00678"
                     /db_xref="CDD:128922"
     CDS             1..303
                     /gene="Rnf146"
                     /coded_by="XM_034339350.2:205..1116"
                     /db_xref="GeneID:117611404"
ORIGIN      
        1 maqaklnsqe etsgtlndke kesdekegtt tvlecavclq pciyparlpc nhiycylcvk
       61 gvanqskrcp mcrqeippdf lnrpqlvevd eaqkesehfe eeyqwfyegr ngwwkydsrt
      121 sqdlediynl gwwqydqrts heletaykqg krncelliag flyiadfgsm lqlrrndpsr
      181 rrkikrdlyn vpkkgvaglr lnhqdeeiir evrgaerpas pasddmgtgd gtntpvppsn
      241 tpqtpaggta sgdatplndr meqrdslhqv leqmrslvlr ehlssdtdde ldedeetsth
      301 ptl
//
LOCUS       XP_034195246             288 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger protein 146 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195246
VERSION     XP_034195246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="ring finger protein 146 isoform X3"
                     /calculated_mol_wt=32666
     Region          33..82
                     /region_name="RING-HC_RNF146"
                     /note="RING finger, HC subclass, found in RING finger
                     protein 146 (RNF146) and similar proteins; cd16546"
                     /db_xref="CDD:438208"
     Site            order(36..37,39,68,70..71)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438208"
     Region          103..175
                     /region_name="WWE"
                     /note="Domain in Deltex and TRIP12 homologues. Possibly
                     involved in regulation of ubiquitin-mediated proteolysis;
                     smart00678"
                     /db_xref="CDD:128922"
     CDS             1..288
                     /gene="Rnf146"
                     /coded_by="XM_034339355.2:204..1070"
                     /db_xref="GeneID:117611404"
ORIGIN      
        1 maqaklnsqe etsgtlndke kesdekegtt tvlecavclq pciyparlpc nhiycylcvk
       61 gvanqskrcp mcrqeippdf lnrpqlvevd eaqkesehfe eeyqwfyegr ngwwqydqrt
      121 sheletaykq gkrncellia gflyiadfgs mlqlrrndps rrrkikrdly nvpkkgvagl
      181 rlnhqdeeii revrgaerpa spasddmgim fstgdgtntp vppsntpqtp aggtasgdat
      241 plndrmeqrd slhqvleqmr slvlrehlss dtddeldede etsthptl
//
LOCUS       XP_034195250             284 aa            linear   INV 03-SEP-2025
DEFINITION  ring finger protein 146 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195250
VERSION     XP_034195250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339359.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="ring finger protein 146 isoform X4"
                     /calculated_mol_wt=32188
     Region          33..82
                     /region_name="RING-HC_RNF146"
                     /note="RING finger, HC subclass, found in RING finger
                     protein 146 (RNF146) and similar proteins; cd16546"
                     /db_xref="CDD:438208"
     Site            order(36..37,39,68,70..71)
                     /site_type="other"
                     /note="E2 binding site [polypeptide binding]"
                     /db_xref="CDD:438208"
     Region          103..175
                     /region_name="WWE"
                     /note="Domain in Deltex and TRIP12 homologues. Possibly
                     involved in regulation of ubiquitin-mediated proteolysis;
                     smart00678"
                     /db_xref="CDD:128922"
     CDS             1..284
                     /gene="Rnf146"
                     /coded_by="XM_034339359.2:203..1057"
                     /db_xref="GeneID:117611404"
ORIGIN      
        1 maqaklnsqe etsgtlndke kesdekegtt tvlecavclq pciyparlpc nhiycylcvk
       61 gvanqskrcp mcrqeippdf lnrpqlvevd eaqkesehfe eeyqwfyegr ngwwqydqrt
      121 sheletaykq gkrncellia gflyiadfgs mlqlrrndps rrrkikrdly nvpkkgvagl
      181 rlnhqdeeii revrgaerpa spasddmgtg dgtntpvpps ntpqtpaggt asgdatplnd
      241 rmeqrdslhq vleqmrslvl rehlssdtdd eldedeetst hptl
//
LOCUS       XP_034195410             865 aa            linear   INV 03-SEP-2025
DEFINITION  DNA repair endonuclease XPF mei-9 [Osmia lignaria lignaria].
ACCESSION   XP_034195410
VERSION     XP_034195410.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339519.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..865
                     /product="DNA repair endonuclease XPF mei-9"
                     /calculated_mol_wt=98769
     Region          83..833
                     /region_name="rad1"
                     /note="DNA repair protein (rad1); TIGR00596"
                     /db_xref="CDD:273163"
     CDS             1..865
                     /gene="mei-9"
                     /coded_by="XM_034339519.2:521..3118"
                     /db_xref="GeneID:117611555"
ORIGIN      
        1 mleyenqmfl eiihedglvv vakglgletv fanvlrayld pgnliivlgt tnhdeqyfid
       61 tlkglgnkql prvvtfecss derevmyleg gvlfmsgril vvdllknrvp lklvtgilvy
      121 rahnilnsyq eafalrlyrq snktgfikaf tnsalaftvg faqverimka lfvkklylwp
      181 rfhtlvnssl gkhkpdviel hvkitskmln iqtslldimn yvikelkrln kyldldeltv
      241 enaiskkfhk qlqlqldpmw hqlsatskql lsdlktlrgl lacltyedcv sfyamlsclr
      301 tmeyavknsg wlmldsvdtl fqsakervyn ekdelkpevt pkwtaltevl leiqdqsktk
      361 adkenektli lvhnrnlcyq lknyltmgak eyllyeamkk lsrkelqkpp tekntkeest
      421 saektddnen dekdtymltl sqkvpeesqs dlatedekhq tlfeecsqia didltnlttn
      481 apiiliqslk kdgdpmalqr alaehmpsni vmyvadisav rqievyqnnn psidlkiyfl
      541 iyggsveeqd yltslrreke afhtlintkt kmvipddqdg ksedclslai qtdtdaetnt
      601 rkggipiqpe vpntiivdmr efrselpail ytrgmkiepi tlqvgdyils pdicverksv
      661 sdligslnng rlynqaiamt rhyskamlli efdpnkpfcf qgyyyaskdi qnmfitsklq
      721 lltlhfprlk lvwspgphat aqlfeelkqg reqpdaakaa qigmeetkqi avekfnpriq
      781 dfvsklpgit tknlqsvlsk gqsldhlnkl tkeeltemie nkneaqmlyn afhdksspie
      841 eksntankkt afrgrgkrlf tkikq
//
LOCUS       XP_034195411             540 aa            linear   INV 03-SEP-2025
DEFINITION  putative inactive peptidyl-prolyl cis-trans isomerase-like 6
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195411
VERSION     XP_034195411.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339520.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="putative inactive peptidyl-prolyl cis-trans
                     isomerase-like 6 isoform X1"
                     /calculated_mol_wt=63180
     Region          151..309
                     /region_name="Pro_isomerase"
                     /note="Cyclophilin type peptidyl-prolyl cis-trans
                     isomerase/CLD; pfam00160"
                     /db_xref="CDD:459694"
     CDS             1..540
                     /gene="LOC117611556"
                     /coded_by="XM_034339520.2:1..1623"
                     /db_xref="GeneID:117611556"
ORIGIN      
        1 mmqnkvksal fktdccsslf endyikikiv giintsafqk aricaeklyq hmsfkftlpq
       61 iiemfqidwh eyiekmkrqi ggkmwslkrp vavfinnefi gsdmeffhyi sedyifslpf
      121 gieyyeniai erckqfmeks kriyvyftft innriagsfv fmlysdllpl tcqhflnlcn
      181 gydevkhcyr dayyintrvh rivkngwiqc gdaelseise dragnvpvip desyciphdr
      241 rgvlsmandg khcnqfqfiv clkpnpwmnh yyvafgqlid gartlkiles istyyehpik
      301 qililqcgey cleeepkmea esqvflkhqp pssiegeatr iisdnmfdfy citpwldniv
      361 dvidvrdtas llmverylng lyclstdymp gmdmrvykkl ssvsiakrnt tvklyqllrn
      421 fqpdnmtele krifisdise iiiayifyhe hnefclkhis mdthriihki lenahkiaqk
      481 vvekaekrsl isrecgslni tkmfetcmqd ilpsenclll lqdilnkail cliqvidvqe
//
LOCUS       XP_034195412             455 aa            linear   INV 03-SEP-2025
DEFINITION  putative inactive peptidyl-prolyl cis-trans isomerase-like 6
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195412
VERSION     XP_034195412.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339521.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..455
                     /product="putative inactive peptidyl-prolyl cis-trans
                     isomerase-like 6 isoform X2"
                     /calculated_mol_wt=53396
     Region          136..309
                     /region_name="cyclophilin"
                     /note="cyclophilin-type peptidylprolyl cis- trans
                     isomerases. This family contains eukaryotic, bacterial and
                     archeal proteins which exhibit a peptidylprolyl cis- trans
                     isomerases activity (PPIase, Rotamase) and in addition
                     bind the immunosuppressive drug...; cl00197"
                     /db_xref="CDD:469651"
     Site            order(201,203,206..207,209,247..248,257,259,267..268,272)
                     /site_type="active"
                     /db_xref="CDD:238194"
     CDS             1..455
                     /gene="LOC117611556"
                     /coded_by="XM_034339521.2:1..1368"
                     /db_xref="GeneID:117611556"
ORIGIN      
        1 mmqnkvksal fktdccsslf endyikikiv giintsafqk aricaeklyq hmsfkftlpq
       61 iiemfqidwh eyiekmkrqi ggkmwslkrp vavfinnefi gsdmeffhyi sedyifslpf
      121 gieyyeniai erckqfmeks kriyvyftft innriagsfv fmlysdllpl tcqhflnlcn
      181 gydevkhcyr dayyintrvh rivkngwiqc gdaelseise dragnvpvip desyciphdr
      241 rgvlsmandg khcnqfqfiv clkpnpwmnh yyvafgqlid gartlkiles istyyehpik
      301 qililqcgey cleeepkmea esqvflkhqp pssiegeatr iisdnmfdfy citpwldniv
      361 dvidvrdtas llmverylng lyclstdymp gmdmrvykkl ssvsiakrnt tvklyqllrn
      421 fqpdnmtele krifisdise iiiayifyhe hnenv
//
LOCUS       XP_034195413             417 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein 277 [Osmia lignaria lignaria].
ACCESSION   XP_034195413
VERSION     XP_034195413.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339522.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195413.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..417
                     /product="zinc finger protein 277"
                     /calculated_mol_wt=49541
     Region          157..244
                     /region_name="zf-C2H2_2"
                     /note="C2H2 type zinc-finger (2 copies); pfam12756"
                     /db_xref="CDD:463690"
     Region          293..382
                     /region_name="zf-C2H2_2"
                     /note="C2H2 type zinc-finger (2 copies); pfam12756"
                     /db_xref="CDD:463690"
     CDS             1..417
                     /gene="LOC117611557"
                     /coded_by="XM_034339522.2:118..1371"
                     /db_xref="GeneID:117611557"
ORIGIN      
        1 mnseesqkns lsdnvcnqvs tvyksqfvgl ddkpyatkcl lcnlkfvlpq neeellihlf
       61 eehrlvigdv wkiaslksyi hywsvkfkee pvtnfcttvl mnctpdgqpc eneryfllsd
      121 cifedktlrd eihraklewv ltmqakertd tdfkrgcilc rmefsglrin yvkhlaqkhn
      181 lylgkpdnlv fidefldkiq stienlvciy ceklfkdrtv lkehmrkklh krinpnnkey
      241 dkfyinnyle pgkswrhkqi yfkgkraedq ssenededtw sdwdddegvs isclfckynd
      301 khfhlilehm ktnhdfdfkv askdltfyqk vkvvnyvrrq iylqqcifce vkvndvlehm
      361 keqshfkipt qkswdqpefy fpmcendsfl yhldtsdssd gesdvenltk smceisk
//
LOCUS       XP_034195326             489 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195326
VERSION     XP_034195326.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339435.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="beta-1,4-glucuronyltransferase 1 isoform X1"
                     /calculated_mol_wt=56791
     Region          152..477
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..489
                     /gene="LOC117611489"
                     /coded_by="XM_034339435.2:119..1588"
                     /db_xref="GeneID:117611489"
ORIGIN      
        1 mygtrvqrqr lartdfairl ppagqvclac qhtsmvqfgc rpwnlslksv ivlaisnill
       61 tflllqsetc vpdevprdme snvitewnlg tlikeqqsec vtqdyqpalp adanslkmnv
      121 klgrwdarrm frtfdhvlvg ssfvelsqay rvclatqssv eklhslvqaa hqwtgpmsva
      181 lyaagdeefe vlqkylvylr rcyepirerv sfslavprsr ppkkqpfehe lpdnvdcakp
      241 egtlnelmng isneqtnwri hnvypqnhmr nlarkncqse yvfltdvdiv psynltgald
      301 eflrtdscdk cayviptyel dsrvrfpqnk telvrlarkg larpfhqkvf ihnqfatnft
      361 rwildtvpnh kndenvrsgk vyishdvtnf eflyepfyva kdivpphder fmgygytrnt
      421 qvyemyvagy qfkvlspvft ghwglqtrkt rpawrerqns anrkhfetfk kevfarymrd
      481 plkmmknak
//
LOCUS       XP_034195333             453 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195333
VERSION     XP_034195333.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339442.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="beta-1,4-glucuronyltransferase 1 isoform X2"
                     /calculated_mol_wt=52736
     Region          116..441
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..453
                     /gene="LOC117611489"
                     /coded_by="XM_034339442.2:134..1495"
                     /db_xref="GeneID:117611489"
ORIGIN      
        1 mfgcrpwnls lksvivlais nilltflllq setcvpdevp rdmesnvite wnlgtlikeq
       61 qsecvtqdyq palpadansl kmnvklgrwd arrmfrtfdh vlvgssfvel sqayrvclat
      121 qssveklhsl vqaahqwtgp msvalyaagd eefevlqkyl vylrrcyepi rervsfslav
      181 prsrppkkqp fehelpdnvd cakpegtlne lmngisneqt nwrihnvypq nhmrnlarkn
      241 cqseyvfltd vdivpsynlt galdeflrtd scdkcayvip tyeldsrvrf pqnktelvrl
      301 arkglarpfh qkvfihnqfa tnftrwildt vpnhkndenv rsgkvyishd vtnfeflyep
      361 fyvakdivpp hderfmgygy trntqvyemy vagyqfkvls pvftghwglq trktrpawre
      421 rqnsanrkhf etfkkevfar ymrdplkmmk nak
//
LOCUS       XP_034195339             411 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195339
VERSION     XP_034195339.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339448.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="beta-1,4-glucuronyltransferase 1 isoform X3"
                     /calculated_mol_wt=48022
     Region          74..399
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..411
                     /gene="LOC117611489"
                     /coded_by="XM_034339448.2:687..1922"
                     /db_xref="GeneID:117611489"
ORIGIN      
        1 mesnvitewn lgtlikeqqs ecvtqdyqpa lpadanslkm nvklgrwdar rmfrtfdhvl
       61 vgssfvelsq ayrvclatqs sveklhslvq aahqwtgpms valyaagdee fevlqkylvy
      121 lrrcyepire rvsfslavpr srppkkqpfe helpdnvdca kpegtlnelm ngisneqtnw
      181 rihnvypqnh mrnlarkncq seyvfltdvd ivpsynltga ldeflrtdsc dkcayvipty
      241 eldsrvrfpq nktelvrlar kglarpfhqk vfihnqfatn ftrwildtvp nhkndenvrs
      301 gkvyishdvt nfeflyepfy vakdivpphd erfmgygytr ntqvyemyva gyqfkvlspv
      361 ftghwglqtr ktrpawrerq nsanrkhfet fkkevfarym rdplkmmkna k
//
LOCUS       XP_034195348             411 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195348
VERSION     XP_034195348.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339457.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="beta-1,4-glucuronyltransferase 1 isoform X3"
                     /calculated_mol_wt=48022
     Region          74..399
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..411
                     /gene="LOC117611489"
                     /coded_by="XM_034339457.2:678..1913"
                     /db_xref="GeneID:117611489"
ORIGIN      
        1 mesnvitewn lgtlikeqqs ecvtqdyqpa lpadanslkm nvklgrwdar rmfrtfdhvl
       61 vgssfvelsq ayrvclatqs sveklhslvq aahqwtgpms valyaagdee fevlqkylvy
      121 lrrcyepire rvsfslavpr srppkkqpfe helpdnvdca kpegtlnelm ngisneqtnw
      181 rihnvypqnh mrnlarkncq seyvfltdvd ivpsynltga ldeflrtdsc dkcayvipty
      241 eldsrvrfpq nktelvrlar kglarpfhqk vfihnqfatn ftrwildtvp nhkndenvrs
      301 gkvyishdvt nfeflyepfy vakdivpphd erfmgygytr ntqvyemyva gyqfkvlspv
      361 ftghwglqtr ktrpawrerq nsanrkhfet fkkevfarym rdplkmmkna k
//
LOCUS       XP_034195344             411 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195344
VERSION     XP_034195344.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339453.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="beta-1,4-glucuronyltransferase 1 isoform X3"
                     /calculated_mol_wt=48022
     Region          74..399
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..411
                     /gene="LOC117611489"
                     /coded_by="XM_034339453.2:548..1783"
                     /db_xref="GeneID:117611489"
ORIGIN      
        1 mesnvitewn lgtlikeqqs ecvtqdyqpa lpadanslkm nvklgrwdar rmfrtfdhvl
       61 vgssfvelsq ayrvclatqs sveklhslvq aahqwtgpms valyaagdee fevlqkylvy
      121 lrrcyepire rvsfslavpr srppkkqpfe helpdnvdca kpegtlnelm ngisneqtnw
      181 rihnvypqnh mrnlarkncq seyvfltdvd ivpsynltga ldeflrtdsc dkcayvipty
      241 eldsrvrfpq nktelvrlar kglarpfhqk vfihnqfatn ftrwildtvp nhkndenvrs
      301 gkvyishdvt nfeflyepfy vakdivpphd erfmgygytr ntqvyemyva gyqfkvlspv
      361 ftghwglqtr ktrpawrerq nsanrkhfet fkkevfarym rdplkmmkna k
//
LOCUS       XP_034195351             411 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-glucuronyltransferase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195351
VERSION     XP_034195351.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339460.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="beta-1,4-glucuronyltransferase 1 isoform X3"
                     /calculated_mol_wt=48022
     Region          74..399
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..411
                     /gene="LOC117611489"
                     /coded_by="XM_034339460.2:339..1574"
                     /db_xref="GeneID:117611489"
ORIGIN      
        1 mesnvitewn lgtlikeqqs ecvtqdyqpa lpadanslkm nvklgrwdar rmfrtfdhvl
       61 vgssfvelsq ayrvclatqs sveklhslvq aahqwtgpms valyaagdee fevlqkylvy
      121 lrrcyepire rvsfslavpr srppkkqpfe helpdnvdca kpegtlnelm ngisneqtnw
      181 rihnvypqnh mrnlarkncq seyvfltdvd ivpsynltga ldeflrtdsc dkcayvipty
      241 eldsrvrfpq nktelvrlar kglarpfhqk vfihnqfatn ftrwildtvp nhkndenvrs
      301 gkvyishdvt nfeflyepfy vakdivpphd erfmgygytr ntqvyemyva gyqfkvlspv
      361 ftghwglqtr ktrpawrerq nsanrkhfet fkkevfarym rdplkmmkna k
//
LOCUS       XP_034195354             470 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611523 [Osmia lignaria lignaria].
ACCESSION   XP_034195354
VERSION     XP_034195354.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195354.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="uncharacterized protein LOC117611523"
                     /calculated_mol_wt=53240
     CDS             1..470
                     /gene="LOC117611523"
                     /coded_by="XM_034339463.2:104..1516"
                     /db_xref="GeneID:117611523"
ORIGIN      
        1 msdystdsle dtnevsprsp dsllknnvsp nlqpikatnd lshdvacrlk ptnsllprkq
       61 dtgcnettpg igaskdkgtv gsrnaiiasg dtykrrrpas sknrkvdvtd slkrnnidns
      121 kqkkevakrv vaprctkgsa kaftlkqncc skessmpvkr ekkfgnvsts ntrqtmecsy
      181 lelhcrrfsg pwytvrspkg stlsvwgdsh qeeitddetr slasprddqs elsfyrrlie
      241 asndplasai rssetvhqpd lqdtdllsss fsfdervkqp sddkskksvg yrpytieeyk
      301 tlsipkldrs lgpdkvemqa krewlmrrrs ygnsvsarnc qrmlmqaqki klkddiaekc
      361 flpplkdqem daktsdnsms smifedqgst sktksehsrk tykiskvtlp rrskkferts
      421 sfcsslnsrd fiedsylesl rqrhlyekem vdriinralc srehskqlaa
//
LOCUS       XP_034195361             470 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611523 [Osmia lignaria lignaria].
ACCESSION   XP_034195361
VERSION     XP_034195361.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339470.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195361.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="uncharacterized protein LOC117611523"
                     /calculated_mol_wt=53240
     CDS             1..470
                     /gene="LOC117611523"
                     /coded_by="XM_034339470.2:196..1608"
                     /db_xref="GeneID:117611523"
ORIGIN      
        1 msdystdsle dtnevsprsp dsllknnvsp nlqpikatnd lshdvacrlk ptnsllprkq
       61 dtgcnettpg igaskdkgtv gsrnaiiasg dtykrrrpas sknrkvdvtd slkrnnidns
      121 kqkkevakrv vaprctkgsa kaftlkqncc skessmpvkr ekkfgnvsts ntrqtmecsy
      181 lelhcrrfsg pwytvrspkg stlsvwgdsh qeeitddetr slasprddqs elsfyrrlie
      241 asndplasai rssetvhqpd lqdtdllsss fsfdervkqp sddkskksvg yrpytieeyk
      301 tlsipkldrs lgpdkvemqa krewlmrrrs ygnsvsarnc qrmlmqaqki klkddiaekc
      361 flpplkdqem daktsdnsms smifedqgst sktksehsrk tykiskvtlp rrskkferts
      421 sfcsslnsrd fiedsylesl rqrhlyekem vdriinralc srehskqlaa
//
LOCUS       XP_034195366             470 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611523 [Osmia lignaria lignaria].
ACCESSION   XP_034195366
VERSION     XP_034195366.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339475.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195366.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..470
                     /product="uncharacterized protein LOC117611523"
                     /calculated_mol_wt=53240
     CDS             1..470
                     /gene="LOC117611523"
                     /coded_by="XM_034339475.2:199..1611"
                     /db_xref="GeneID:117611523"
ORIGIN      
        1 msdystdsle dtnevsprsp dsllknnvsp nlqpikatnd lshdvacrlk ptnsllprkq
       61 dtgcnettpg igaskdkgtv gsrnaiiasg dtykrrrpas sknrkvdvtd slkrnnidns
      121 kqkkevakrv vaprctkgsa kaftlkqncc skessmpvkr ekkfgnvsts ntrqtmecsy
      181 lelhcrrfsg pwytvrspkg stlsvwgdsh qeeitddetr slasprddqs elsfyrrlie
      241 asndplasai rssetvhqpd lqdtdllsss fsfdervkqp sddkskksvg yrpytieeyk
      301 tlsipkldrs lgpdkvemqa krewlmrrrs ygnsvsarnc qrmlmqaqki klkddiaekc
      361 flpplkdqem daktsdnsms smifedqgst sktksehsrk tykiskvtlp rrskkferts
      421 sfcsslnsrd fiedsylesl rqrhlyekem vdriinralc srehskqlaa
//
LOCUS       XP_034195304             406 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611459 [Osmia lignaria lignaria].
ACCESSION   XP_034195304
VERSION     XP_034195304.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339413.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="uncharacterized protein LOC117611459"
                     /calculated_mol_wt=46988
     Region          41..324
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..406
                     /gene="LOC117611459"
                     /coded_by="XM_034339413.2:186..1406"
                     /db_xref="GeneID:117611459"
ORIGIN      
        1 mevkhlkidk hlfekalrrk sdetvqvlei knnpisdigl nflsdlfevs vrytvtskke
       61 ktkserstdv fikieplndv prdlvrqqdl flielkvlrd vlpkikqlms cqlgphlwyg
      121 sdnppvlmme dlknrgfimk nrqkglsfeh cnlaiqqiak lhagsvavfe kdpetieffr
      181 nggivspkcp tsylrlmevs lirisneirn wedkkcanaa dklikvaeti gtrcidayky
      241 dpdefcvlnh gdcwinnmmf kenekgeptd lllvdyqmav ytspvidliy flnicpefsv
      301 kydnddyflk iylntlketm rsigcktkpp tmqqlkdalh krriyavfsg ivlylrmman
      361 kedtedftvv lkklsgetkm dvfrnpdavk lahkmipvmn ergyfd
//
LOCUS       XP_034195309             406 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611459 [Osmia lignaria lignaria].
ACCESSION   XP_034195309
VERSION     XP_034195309.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339418.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="uncharacterized protein LOC117611459"
                     /calculated_mol_wt=46988
     Region          41..324
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..406
                     /gene="LOC117611459"
                     /coded_by="XM_034339418.2:220..1440"
                     /db_xref="GeneID:117611459"
ORIGIN      
        1 mevkhlkidk hlfekalrrk sdetvqvlei knnpisdigl nflsdlfevs vrytvtskke
       61 ktkserstdv fikieplndv prdlvrqqdl flielkvlrd vlpkikqlms cqlgphlwyg
      121 sdnppvlmme dlknrgfimk nrqkglsfeh cnlaiqqiak lhagsvavfe kdpetieffr
      181 nggivspkcp tsylrlmevs lirisneirn wedkkcanaa dklikvaeti gtrcidayky
      241 dpdefcvlnh gdcwinnmmf kenekgeptd lllvdyqmav ytspvidliy flnicpefsv
      301 kydnddyflk iylntlketm rsigcktkpp tmqqlkdalh krriyavfsg ivlylrmman
      361 kedtedftvv lkklsgetkm dvfrnpdavk lahkmipvmn ergyfd
//
LOCUS       XP_034195314             406 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611459 [Osmia lignaria lignaria].
ACCESSION   XP_034195314
VERSION     XP_034195314.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339423.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="uncharacterized protein LOC117611459"
                     /calculated_mol_wt=46988
     Region          41..324
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..406
                     /gene="LOC117611459"
                     /coded_by="XM_034339423.2:214..1434"
                     /db_xref="GeneID:117611459"
ORIGIN      
        1 mevkhlkidk hlfekalrrk sdetvqvlei knnpisdigl nflsdlfevs vrytvtskke
       61 ktkserstdv fikieplndv prdlvrqqdl flielkvlrd vlpkikqlms cqlgphlwyg
      121 sdnppvlmme dlknrgfimk nrqkglsfeh cnlaiqqiak lhagsvavfe kdpetieffr
      181 nggivspkcp tsylrlmevs lirisneirn wedkkcanaa dklikvaeti gtrcidayky
      241 dpdefcvlnh gdcwinnmmf kenekgeptd lllvdyqmav ytspvidliy flnicpefsv
      301 kydnddyflk iylntlketm rsigcktkpp tmqqlkdalh krriyavfsg ivlylrmman
      361 kedtedftvv lkklsgetkm dvfrnpdavk lahkmipvmn ergyfd
//
LOCUS       XP_034195317             406 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611459 [Osmia lignaria lignaria].
ACCESSION   XP_034195317
VERSION     XP_034195317.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339426.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="uncharacterized protein LOC117611459"
                     /calculated_mol_wt=46988
     Region          41..324
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..406
                     /gene="LOC117611459"
                     /coded_by="XM_034339426.2:185..1405"
                     /db_xref="GeneID:117611459"
ORIGIN      
        1 mevkhlkidk hlfekalrrk sdetvqvlei knnpisdigl nflsdlfevs vrytvtskke
       61 ktkserstdv fikieplndv prdlvrqqdl flielkvlrd vlpkikqlms cqlgphlwyg
      121 sdnppvlmme dlknrgfimk nrqkglsfeh cnlaiqqiak lhagsvavfe kdpetieffr
      181 nggivspkcp tsylrlmevs lirisneirn wedkkcanaa dklikvaeti gtrcidayky
      241 dpdefcvlnh gdcwinnmmf kenekgeptd lllvdyqmav ytspvidliy flnicpefsv
      301 kydnddyflk iylntlketm rsigcktkpp tmqqlkdalh krriyavfsg ivlylrmman
      361 kedtedftvv lkklsgetkm dvfrnpdavk lahkmipvmn ergyfd
//
LOCUS       XP_034194247             224 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610694 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194247
VERSION     XP_034194247.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338356.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..224
                     /product="uncharacterized protein LOC117610694 isoform X1"
                     /calculated_mol_wt=25587
     Region          80..>145
                     /region_name="Spaetzle"
                     /note="cl29083"
                     /db_xref="CDD:475150"
     CDS             1..224
                     /gene="LOC117610694"
                     /coded_by="XM_034338356.2:3898..4572"
                     /db_xref="GeneID:117610694"
ORIGIN      
        1 mvpvgiratl llvvlltavl cprlaaannl tlsfsprktr ehpfrghisr rtqtlddptk
       61 ryreilrqeg gehglpvdcc ptveemvepv ggrnrenmyv qlyrdgqnaq rffeysckpe
      121 vldkpcrfid rkfsnqsrcv qkfsytyaiv ensgstggke ehgrhrhrer liptfagntv
      181 ggsmwtldyi kvrsgcscev spkpkkkkkf kksrprdqdf dlep
//
LOCUS       XP_034194257             223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610694 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194257
VERSION     XP_034194257.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="uncharacterized protein LOC117610694 isoform X2"
                     /calculated_mol_wt=25563
     Region          79..>144
                     /region_name="Spaetzle"
                     /note="cl29083"
                     /db_xref="CDD:475150"
     CDS             1..223
                     /gene="LOC117610694"
                     /coded_by="XM_034338366.2:4140..4811"
                     /db_xref="GeneID:117610694"
ORIGIN      
        1 mrievlntlv avvlltavlc prlaaannlt lsfsprktre hpfrghisrr tqtlddptkr
       61 yreilrqegg ehglpvdccp tveemvepvg grnrenmyvq lyrdgqnaqr ffeysckpev
      121 ldkpcrfidr kfsnqsrcvq kfsytyaive nsgstggkee hgrhrhrerl iptfagntvg
      181 gsmwtldyik vrsgcscevs pkpkkkkkfk ksrprdqdfd lep
//
LOCUS       XP_076546590             223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610694 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546590
VERSION     XP_076546590.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690475.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="uncharacterized protein LOC117610694 isoform X2"
                     /calculated_mol_wt=25563
     Region          79..>144
                     /region_name="Spaetzle"
                     /note="cl29083"
                     /db_xref="CDD:475150"
     CDS             1..223
                     /gene="LOC117610694"
                     /coded_by="XM_076690475.1:382..1053"
                     /db_xref="GeneID:117610694"
ORIGIN      
        1 mrievlntlv avvlltavlc prlaaannlt lsfsprktre hpfrghisrr tqtlddptkr
       61 yreilrqegg ehglpvdccp tveemvepvg grnrenmyvq lyrdgqnaqr ffeysckpev
      121 ldkpcrfidr kfsnqsrcvq kfsytyaive nsgstggkee hgrhrhrerl iptfagntvg
      181 gsmwtldyik vrsgcscevs pkpkkkkkfk ksrprdqdfd lep
//
LOCUS       XP_034195418             275 aa            linear   INV 03-SEP-2025
DEFINITION  Rieske iron-sulfur protein [Osmia lignaria lignaria].
ACCESSION   XP_034195418
VERSION     XP_034195418.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339527.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195418.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="Rieske iron-sulfur protein"
                     /calculated_mol_wt=29852
     Region          1..71
                     /region_name="Ubiq-Cytc-red_N"
                     /note="Ubiquinol-cytochrome c reductase 8 kDa, N-terminal;
                     pfam09165"
                     /db_xref="CDD:462698"
     Region          81..146
                     /region_name="UCR_TM"
                     /note="Ubiquinol cytochrome reductase transmembrane
                     region; pfam02921"
                     /db_xref="CDD:460747"
     Region          152..275
                     /region_name="Rieske_cytochrome_bc1"
                     /note="Iron-sulfur protein (ISP) component of the bc(1)
                     complex family, Rieske domain; The Rieske domain is a
                     [2Fe-2S] cluster binding domain involved in electron
                     transfer. The bc(1) complex is a multisubunit enzyme found
                     in many different organisms including...; cd03470"
                     /db_xref="CDD:239552"
     Site            order(218,220..221,237,240,242)
                     /site_type="other"
                     /note="[2Fe-2S] cluster binding site [ion binding]"
                     /db_xref="CDD:239552"
     CDS             1..275
                     /gene="RFeSP"
                     /coded_by="XM_034339527.2:77..904"
                     /db_xref="GeneID:117611562"
ORIGIN      
        1 mnvvakssnl spflrstvtv vtngtqpvvs saavvkpkii gkstvklllk esmlqslitg
       61 plrvssgiga sllavqrrma htdiqwpdft dyrqdstkdp tvkskesaas rktftyvmtt
      121 amgvgcmysa ksavhnavsl laatkdvlal akievkldti pegksvifkw rgkplfvrhr
      181 sakeiekesq vditnlrdpq ddkdrvkqpe wlivlgvcth lgcvpianag efggyycpch
      241 gshydasgri rkgpaplnle vppyefidqk tvlvg
//
LOCUS       XP_034195419             173 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L24-like [Osmia lignaria lignaria].
ACCESSION   XP_034195419
VERSION     XP_034195419.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339528.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..173
                     /product="ribosomal protein L24-like"
                     /calculated_mol_wt=20766
     Region          1..130
                     /region_name="Ribosomal_L24e_L24"
                     /note="Ribosomal protein L24e/L24 is a ribosomal protein
                     found in eukaryotes (L24) and in archaea (L24e, distinct
                     from archaeal L24). L24e/L24 is located on the surface of
                     the large subunit, adjacent to proteins L14 and L3, and
                     near the translation factor...; cl00909"
                     /db_xref="CDD:469985"
     Region          2..64
                     /region_name="Ribosomal_L24e"
                     /note="Ribosomal protein L24e; pfam01246"
                     /db_xref="CDD:460131"
     CDS             1..173
                     /gene="RpL24-like"
                     /coded_by="XM_034339528.2:78..599"
                     /db_xref="GeneID:117611563"
ORIGIN      
        1 mrietcyfcs sriypghgiq fvrndckifk fcrskchaaf kkkknprkvr wtkayrktvg
       61 kelavdpafe fekrrnipvk ynrelwnkti eamrkvetir qrrqnlhimq rlrkgreleq
      121 erdvkevqrd lslirspaag lkerkkleea aeqeemmqes ndeqepeeme mns
//
LOCUS       XP_034195539             263 aa            linear   INV 03-SEP-2025
DEFINITION  trypsin 3A1-like [Osmia lignaria lignaria].
ACCESSION   XP_034195539
VERSION     XP_034195539.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339648.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195539.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="trypsin 3A1-like"
                     /calculated_mol_wt=29145
     Region          26..243
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            order(60,105,201)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(195,216,218)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..263
                     /gene="LOC117611632"
                     /coded_by="XM_034339648.2:164..955"
                     /db_xref="GeneID:117611632"
ORIGIN      
        1 mfkkilllvc vifcdtktvf entkyvdire ypyhvsiekh gihacsgalm heswivtvas
       61 cvfrtdpstv tvrvrtstls tggdelkvsn vvvhenfney vllndialik lkipvqfgek
      121 llpigvpene dyeldygtmc fvtgwkqtlp gpaesrlsvt tvplmnrssc vskmppyqpv
      181 lqsmlcagnm tqgvetcqgd pgaplmegqt ligvlsyglg cktmihpavy vrvssyltwi
      241 stntdvhytr vtrkfsskkt lph
//
LOCUS       XP_076546591             251 aa            linear   INV 03-SEP-2025
DEFINITION  trypsin-1-like [Osmia lignaria lignaria].
ACCESSION   XP_076546591
VERSION     XP_076546591.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690476.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..251
                     /product="trypsin-1-like"
                     /calculated_mol_wt=26670
     Region          30..248
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            30
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(69,112,206)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(200,221,223)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..251
                     /gene="LOC143305727"
                     /coded_by="XM_076690476.1:115..870"
                     /db_xref="GeneID:143305727"
ORIGIN      
        1 mlrvvvlsvf iigcfgtalr gypplpdthi vggveadirq hpyqlslqts ghvcgasiis
       61 sewavtaahc vgsvasrysl ragnsnknlg trylidtivi hpnydrtiid hdvallkvsg
      121 gfsfgptvqp ikladeevpa dtmvnvtgwg alrqggmtss qlmrvsipiv trtkceeayq
      181 glnaitsrmi cagyteggkd scqgdsggpl tasetlygiv swgygcakpe ypgvytnvan
      241 lrswikeisg v
//
LOCUS       XP_034195602             399 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611666 [Osmia lignaria lignaria].
ACCESSION   XP_034195602
VERSION     XP_034195602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..399
                     /product="uncharacterized protein LOC117611666"
                     /calculated_mol_wt=46186
     Region          49..310
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..399
                     /gene="LOC117611666"
                     /coded_by="XM_034339711.2:90..1289"
                     /db_xref="GeneID:117611666"
ORIGIN      
        1 mceqvvdyrv cldpliekvt sslnqnfdnv hyelstpdds fffsdmffvk itvnqsegkn
       61 etinvvvkkp sqmdhvrevl esdaqfhnei lfyekyakgd nelprcyyte ekppmdtvil
      121 lenitqrgyk lshwkfnvpl eytlaafrri aryhakgyam keqhpdqffd ivskiwqtrf
      181 nqdstlkgvl natvtrpvey lrktgsekdf cdkfeekcqd mfnnvmlkiv epeeplatic
      241 hgdftinntf fkrengqlna mlidfalmry gspvidlstf lclhcteald knlldnvlka
      301 yhdalieclk ehgadcekys yqafyedykk rgifgyfiav fflsivmnka ttspdelmkm
      361 speesglilr elggdevsei lanmllklke fgclddiis
//
LOCUS       XP_034191640             438 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor-like protein slowdown [Osmia lignaria
            lignaria].
ACCESSION   XP_034191640
VERSION     XP_034191640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335749.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..438
                     /product="epidermal growth factor-like protein slowdown"
                     /calculated_mol_wt=50017
     Region          <222..247
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          249..287
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Site            order(249,252,265)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          <279..>425
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..438
                     /gene="slow"
                     /coded_by="XM_034335749.2:158..1474"
                     /db_xref="GeneID:117609428"
ORIGIN      
        1 mssekivptm afvqkifftc sllvlpieiv siehssgsdr ydssshrliw piwfheklsr
       61 irhdrdsrss qqhlfperft stiapqsvgs rhhveqrvhq qrsgttkpek lstvavaeyg
      121 gtraiayagy hnshrhqpre titqsyphlt ttpstytrhh pgrrvcnrsv psstvhhhqn
      181 gqirylnifn ikadtsvflc cpgwtqathl sfgcnkptcp apclnggict spgrctcpkg
      241 ftgnqcqtdv decvtekpcd qlcrnlpgty echcrpgfql qkdgqscrkn dtedtafear
      301 dlevdfheat ttkrpsissh dtenevaddd ldqdyeiilk rltklekqfa kgrkrdtdtt
      361 emsvkvasvv dsinemkrav enmhhmqqei yemrnklrey eletrkmqhl tnrvmelenr
      421 lrlrcratmp inggalnf
//
LOCUS       XP_034191689             140 aa            linear   INV 03-SEP-2025
DEFINITION  protein LLP homolog [Osmia lignaria lignaria].
ACCESSION   XP_034191689
VERSION     XP_034191689.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335798.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="protein LLP homolog"
                     /calculated_mol_wt=16153
     Region          3..124
                     /region_name="Laps"
                     /note="Learning-associated protein; pfam10169"
                     /db_xref="CDD:462976"
     CDS             1..140
                     /gene="LOC117609443"
                     /coded_by="XM_034335798.2:172..594"
                     /db_xref="GeneID:117609443"
ORIGIN      
        1 makslrskwr rkcravkrer ygakeldrlk ktlgisengs qdvemseise iatvidakti
       61 kentkskegt eeddldtmev dsngriynkr tmkdqfgnyp vwmnrrkiak hkkgraksqk
      121 sttgsnkrlt rkqkkttkkk
//
LOCUS       XP_034195177            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611351 [Osmia lignaria lignaria].
ACCESSION   XP_034195177
VERSION     XP_034195177.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339286.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195177.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117611351"
                     /calculated_mol_wt=137977
     CDS             1..1226
                     /gene="LOC117611351"
                     /coded_by="XM_034339286.2:458..4138"
                     /db_xref="GeneID:117611351"
ORIGIN      
        1 mdfngnsyts atggsvcqks rrsndslemn slccdstrnq lqnctehrea crqsgnhgki
       61 cehgkveswr gsfsdgktrn fnennmseyr eqesrydnyg nendtagfdv ngsnepcgrc
      121 ksgfnvnvvl hqesptnqki nmvepkynvs fkrccdennk kweqkrrkrs npisltgsff
      181 nlskmkkckk qetnrskgss vqqktdgsde nssnfrctfe nccgncesyi meipqdnlna
      241 yqnwqnmefe ggntcfdecc cpremkmyss nlslasspqh eqcfvsyaet ekccsfpiea
      301 kcefpsnepk epskhclqrv hfakadnntr tnhsscsfcs rkctpskasl fveigesiad
      361 vcnlrsaqvf qlcidskesl kneqerkeql sarknmsdss rerykskkqh gknakcsvnc
      421 kalpaavtrs ysdritsmer fdnqsveert egspceqtmr nlkssisfqe srdcraqtes
      481 nmymnrktad cnqqcegidh svwedirkak iwsqlqdtyk hlvkmattsi yqimkqsnkl
      541 rkkytkdrsk drekcvpymd rsetpndywn gtlielnncd atycgnvccs lnenlnnglt
      601 eceqslyetc kvcqngqeat slecsscfgg pcdiqenmyp cnegsptyql lptcgivrer
      661 ggsdgpggsn qrrkvsnpna srrptqngre qpgtsgnrrs tnprnqttrk mgsraqerne
      721 mpgydqgtec ntmvidmstv rliepcdppc vtesvsyqks iravrrrety sdaiverpss
      781 drplpvkgkh vpmieeahsp vqplpspkee tkkptrtsml tpkkpdtrsn agqksksvav
      841 tspkvpkpeg nnrnasvssn krdstlkqrp itkaaekvda rssltakkvq ceiiknlrvt
      901 cgnkdskkll eqlclcnpqg tpvapvfsev qvteetgnev alpspitadd apesasleee
      961 reetipieee estpveetye sisaedkeet thieeieest pveeeeestp veeeeestpv
     1021 eeekeitsvd ekeesipeee ieeettedtk evqstaidty aekseaitsi tpltdgavnl
     1081 qdtagtskpm efyrefkggr nyvsvqmqts stiltkilse fqvgnkkrqv lmniklrttl
     1141 dgsndedqqp etdvasasss rgqsmtress rvsmqyyyve qkrsavrerd qrvsmdpaid
     1201 ddrhlsndrg spcnkcsgpc gkkklg
//
LOCUS       XP_034195180            1226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611351 [Osmia lignaria lignaria].
ACCESSION   XP_034195180
VERSION     XP_034195180.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339289.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195180.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1226
                     /product="uncharacterized protein LOC117611351"
                     /calculated_mol_wt=137977
     CDS             1..1226
                     /gene="LOC117611351"
                     /coded_by="XM_034339289.2:443..4123"
                     /db_xref="GeneID:117611351"
ORIGIN      
        1 mdfngnsyts atggsvcqks rrsndslemn slccdstrnq lqnctehrea crqsgnhgki
       61 cehgkveswr gsfsdgktrn fnennmseyr eqesrydnyg nendtagfdv ngsnepcgrc
      121 ksgfnvnvvl hqesptnqki nmvepkynvs fkrccdennk kweqkrrkrs npisltgsff
      181 nlskmkkckk qetnrskgss vqqktdgsde nssnfrctfe nccgncesyi meipqdnlna
      241 yqnwqnmefe ggntcfdecc cpremkmyss nlslasspqh eqcfvsyaet ekccsfpiea
      301 kcefpsnepk epskhclqrv hfakadnntr tnhsscsfcs rkctpskasl fveigesiad
      361 vcnlrsaqvf qlcidskesl kneqerkeql sarknmsdss rerykskkqh gknakcsvnc
      421 kalpaavtrs ysdritsmer fdnqsveert egspceqtmr nlkssisfqe srdcraqtes
      481 nmymnrktad cnqqcegidh svwedirkak iwsqlqdtyk hlvkmattsi yqimkqsnkl
      541 rkkytkdrsk drekcvpymd rsetpndywn gtlielnncd atycgnvccs lnenlnnglt
      601 eceqslyetc kvcqngqeat slecsscfgg pcdiqenmyp cnegsptyql lptcgivrer
      661 ggsdgpggsn qrrkvsnpna srrptqngre qpgtsgnrrs tnprnqttrk mgsraqerne
      721 mpgydqgtec ntmvidmstv rliepcdppc vtesvsyqks iravrrrety sdaiverpss
      781 drplpvkgkh vpmieeahsp vqplpspkee tkkptrtsml tpkkpdtrsn agqksksvav
      841 tspkvpkpeg nnrnasvssn krdstlkqrp itkaaekvda rssltakkvq ceiiknlrvt
      901 cgnkdskkll eqlclcnpqg tpvapvfsev qvteetgnev alpspitadd apesasleee
      961 reetipieee estpveetye sisaedkeet thieeieest pveeeeestp veeeeestpv
     1021 eeekeitsvd ekeesipeee ieeettedtk evqstaidty aekseaitsi tpltdgavnl
     1081 qdtagtskpm efyrefkggr nyvsvqmqts stiltkilse fqvgnkkrqv lmniklrttl
     1141 dgsndedqqp etdvasasss rgqsmtress rvsmqyyyve qkrsavrerd qrvsmdpaid
     1201 ddrhlsndrg spcnkcsgpc gkkklg
//
LOCUS       XP_034195192             385 aa            linear   INV 03-SEP-2025
DEFINITION  NAD kinase 2, mitochondrial isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195192
VERSION     XP_034195192.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339301.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195192.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..385
                     /product="NAD kinase 2, mitochondrial isoform X1"
                     /calculated_mol_wt=43945
     Region          67..>262
                     /region_name="NadK"
                     /note="NAD kinase [Coenzyme transport and metabolism];
                     COG0061"
                     /db_xref="CDD:439831"
     CDS             1..385
                     /gene="LOC117611365"
                     /coded_by="XM_034339301.2:1..1158"
                     /db_xref="GeneID:117611365"
ORIGIN      
        1 mklstclnst syrpqlvlgv srlssleylk frnpdlnedq ilsrikeegq dpqeiltehq
       61 eqisceqnla avlkkldisf ritkrigdat kcvkwadlvi piggdgtfll asklisdnkk
      121 pvlginplvs dkntyftlpy kyttnmesif eklhsgeyel lmrsrirtvm tgeglycrpf
      181 hihekgrtkg ekrsqslirs kgkkisdalq prrrilpwla lnevfigefl avrpitlhlq
      241 vedqegyvfr ssgicvctgt gsrswyrsmn vkpadtvqsi vematgkklt ieetnkllyk
      301 yrqsllypae dlrmtymire myraprcwrn kcypdremcn kitvksygfd agliidgsis
      361 lpfndgasas fdikpeyalm niimt
//
LOCUS       XP_034195198             384 aa            linear   INV 03-SEP-2025
DEFINITION  NAD kinase 2, mitochondrial isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195198
VERSION     XP_034195198.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339307.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195198.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="NAD kinase 2, mitochondrial isoform X2"
                     /calculated_mol_wt=43816
     Region          67..>262
                     /region_name="NadK"
                     /note="NAD kinase [Coenzyme transport and metabolism];
                     COG0061"
                     /db_xref="CDD:439831"
     CDS             1..384
                     /gene="LOC117611365"
                     /coded_by="XM_034339307.2:1..1155"
                     /db_xref="GeneID:117611365"
ORIGIN      
        1 mklstclnst syrpqlvlgv srlssleylk frnpdlnedq ilsrikeegq dpqeiltehq
       61 eqisceqnla avlkkldisf ritkrigdat kcvkwadlvi piggdgtfll asklisdnkk
      121 pvlginplvs dkntyftlpy kyttnmesif eklhsgeyel lmrsrirtvm tgeglycrpf
      181 hihekgrtkg ekrsqslirs kgkkisdalq prrrilpwla lnevfigefl avrpitlhlq
      241 vedqegyvfr ssgicvctgt gsrswyrsmn vkpadtvqsi vematgkklt ieetnkllyk
      301 yrqsllypad lrmtymirem yraprcwrnk cypdremcnk itvksygfda gliidgsisl
      361 pfndgasasf dikpeyalmn iimt
//
LOCUS       XP_034195205             335 aa            linear   INV 03-SEP-2025
DEFINITION  NAD kinase 2, mitochondrial isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195205
VERSION     XP_034195205.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339314.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195205.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..335
                     /product="NAD kinase 2, mitochondrial isoform X3"
                     /calculated_mol_wt=38285
     Region          67..>106
                     /region_name="DAGK_cat"
                     /note="Diacylglycerol kinase catalytic domain; cl01255"
                     /db_xref="CDD:445337"
     CDS             1..335
                     /gene="LOC117611365"
                     /coded_by="XM_034339314.2:1..1008"
                     /db_xref="GeneID:117611365"
ORIGIN      
        1 mklstclnst syrpqlvlgv srlssleylk frnpdlnedq ilsrikeegq dpqeiltehq
       61 eqisceqnla avlkkldisf ritkrigdat kcvkwadlvi piggdgeyel lmrsrirtvm
      121 tgeglycrpf hihekgrtkg ekrsqslirs kgkkisdalq prrrilpwla lnevfigefl
      181 avrpitlhlq vedqegyvfr ssgicvctgt gsrswyrsmn vkpadtvqsi vematgkklt
      241 ieetnkllyk yrqsllypae dlrmtymire myraprcwrn kcypdremcn kitvksygfd
      301 agliidgsis lpfndgasas fdikpeyalm niimt
//
LOCUS       XP_034195456             458 aa            linear   INV 03-SEP-2025
DEFINITION  krueppel isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195456
VERSION     XP_034195456.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339565.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..458
                     /product="krueppel isoform X1"
                     /calculated_mol_wt=50188
     Region          207..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(212,214,216,218..219,222..223,226,240,242,246..247,
                     250..251,254,268,270,272,274..275,278..279,282)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          220..244
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          235..255
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          247..272
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          263..283
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          275..299
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          291..311
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          319..335
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..458
                     /gene="Kr"
                     /coded_by="XM_034339565.2:83..1459"
                     /db_xref="GeneID:117611582"
ORIGIN      
        1 malsylqeaq inaglltgqh ldmkqepekq pvspplnnnt sqvmfpgmgs ttaglsmltp
       61 qqllaasraa almaagipvs lhatlaasps lyghhqtlfe gwapptassp psplhhspgp
      121 vspalstkst srrannavtn nnnnnnnvvt ssrdriskkg qttkrkaqks kadsvqvsve
      181 ggadplsppt sispeagkdg rdkvftcgvc srsfgykhvl qnherthtge kpfecpechk
      241 rftrdhhlkt hmrlhtgekp yhcshcdrqf vqvanlrrhl rvhtgerpya celcaakfsd
      301 snqlkahlli hkgekpfece hcrmrfrrrh hlmhhkcgta mnasgqiprs qvtspslasd
      361 yldedididi dvemeeseat plvtrksnnp vrpahrrlps pvvsasmpmp lnlsgipvdl
      421 peqtepedls mstdthrphs nssngstmsr gndrhtis
//
LOCUS       XP_034195457             436 aa            linear   INV 03-SEP-2025
DEFINITION  krueppel isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195457
VERSION     XP_034195457.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339566.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..436
                     /product="krueppel isoform X2"
                     /calculated_mol_wt=47819
     Region          185..205
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(190,192,194,196..197,200..201,204,218,220,224..225,
                     228..229,232,246,248,250,252..253,256..257,260)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          198..222
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          213..233
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          225..250
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          241..261
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          253..277
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          269..289
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          297..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..436
                     /gene="Kr"
                     /coded_by="XM_034339566.2:356..1666"
                     /db_xref="GeneID:117611582"
ORIGIN      
        1 mkqepekqpv spplnnntsq vmfpgmgstt aglsmltpqq llaasraaal maagipvslh
       61 atlaaspsly ghhqtlfegw apptasspps plhhspgpvs palstkstsr rannavtnnn
      121 nnnnnvvtss rdriskkgqt tkrkaqkska dsvqvsvegg adplspptsi speagkdgrd
      181 kvftcgvcsr sfgykhvlqn herthtgekp fecpechkrf trdhhlkthm rlhtgekpyh
      241 cshcdrqfvq vanlrrhlrv htgerpyace lcaakfsdsn qlkahllihk gekpfecehc
      301 rmrfrrrhhl mhhkcgtamn asgqiprsqv tspslasdyl dedidididv emeeseatpl
      361 vtrksnnpvr pahrrlpspv vsasmpmpln lsgipvdlpe qtepedlsms tdthrphsns
      421 sngstmsrgn drhtis
//
LOCUS       XP_034195458             436 aa            linear   INV 03-SEP-2025
DEFINITION  krueppel isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195458
VERSION     XP_034195458.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339567.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..436
                     /product="krueppel isoform X2"
                     /calculated_mol_wt=47819
     Region          185..205
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(190,192,194,196..197,200..201,204,218,220,224..225,
                     228..229,232,246,248,250,252..253,256..257,260)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          198..222
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          213..233
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          225..250
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          241..261
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          253..277
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          269..289
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          297..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..436
                     /gene="Kr"
                     /coded_by="XM_034339567.2:228..1538"
                     /db_xref="GeneID:117611582"
ORIGIN      
        1 mkqepekqpv spplnnntsq vmfpgmgstt aglsmltpqq llaasraaal maagipvslh
       61 atlaaspsly ghhqtlfegw apptasspps plhhspgpvs palstkstsr rannavtnnn
      121 nnnnnvvtss rdriskkgqt tkrkaqkska dsvqvsvegg adplspptsi speagkdgrd
      181 kvftcgvcsr sfgykhvlqn herthtgekp fecpechkrf trdhhlkthm rlhtgekpyh
      241 cshcdrqfvq vanlrrhlrv htgerpyace lcaakfsdsn qlkahllihk gekpfecehc
      301 rmrfrrrhhl mhhkcgtamn asgqiprsqv tspslasdyl dedidididv emeeseatpl
      361 vtrksnnpvr pahrrlpspv vsasmpmpln lsgipvdlpe qtepedlsms tdthrphsns
      421 sngstmsrgn drhtis
//
LOCUS       XP_034196329             575 aa            linear   INV 03-SEP-2025
DEFINITION  alpha glucosidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034196329
VERSION     XP_034196329.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340438.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196329.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="alpha glucosidase 2"
                     /calculated_mol_wt=65626
     Region          25..472
                     /region_name="AmyAc_maltase"
                     /note="Alpha amylase catalytic domain found in maltase
                     (also known as alpha glucosidase) and related proteins;
                     cd11328"
                     /db_xref="CDD:200467"
     Site            order(83,86,123,126,172,191,195,227,295,356..357)
                     /site_type="active"
                     /db_xref="CDD:200467"
     Site            order(227,295,357)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:200467"
     CDS             1..575
                     /gene="AGLU2"
                     /coded_by="XM_034340438.2:25..1752"
                     /db_xref="GeneID:117611929"
ORIGIN      
        1 mkttnnlvgi vllltvafas aeikdkgwwn htvfyqiypr sfqdsnddgv gdlkgitnkl
       61 shfndigvda iwlspiyksp mvdfgydied flevdpvfgt sadlkelate arkknlklvl
      121 dlvpnhtsdk hkwfqlsinr tgkyadyyiw ndgiivngte erlppnnwvs vfngsawtwn
      181 dqrkqfyyhq fyyqqpdlny nnpdvqqemk nvlrywldqg vdgfridavp hliennitqd
      241 eplfnstynt tlhasynhtl tkdqpetykl vqswrdyvde yasqrneaek vilteaytsw
      301 ehtieyynsg ahvpfnfkfi veadanskps dfrniinkwm qmmpkgqvan wvmgnhdrvr
      361 lgtrypgred qmvmlemllp gvavtyygee igmlddtelr vydfrdgcrt pfqwddttna
      421 gfsnasktwl pvhynyktln lkkqkadats hyklykaltt mrksnvlqng nlsiailndd
      481 vlavvrnipk eavtllinfs seksvtvnlt silpyasakv tltnvgssvq sntnfasskv
      541 tippkgsivl hsgssivscs almilvlalg syfrs
//
LOCUS       XP_034195461             779 aa            linear   INV 03-SEP-2025
DEFINITION  protein zer-1 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034195461
VERSION     XP_034195461.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339570.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..779
                     /product="protein zer-1 homolog"
                     /calculated_mol_wt=89080
     Region          87..110
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          111..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          136..166
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <161..>332
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          167..191
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          192..214
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          215..238
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          271..294
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..779
                     /gene="LOC117611585"
                     /coded_by="XM_034339570.2:352..2691"
                     /db_xref="GeneID:117611585"
ORIGIN      
        1 madlddlfhl dqyigpesla elcfqvickn ldiisvkdkr gyrnlskgli fpseicdkli
       61 eyvlrndphk ehdcfitifq nvsttklkri kivrskitdy avqiitnhkp veleltdcpr
      121 ltersikyin anaenlqsli chgtsviipe eitayqkrgy vfkapnlrtl aleyvtiyas
      181 eynillaglt nlknldlsns fnvdnfdfyh lipnlvslvl ynvniqpqal vnichlknlr
      241 hldisqsnpq qgifenpntv ltdivnglpq ltsldisgtn lagiqivdrg iqavehsfys
      301 efddmanndl cdipglasri nrplqflgly gtsdgacrrr nipakliagn anedqilvaa
      361 hvcmnnkqel lqkvlndlyh vyryenchrm dqalcailea mekhplqkhi qisgsatlfy
      421 ivkmkekgel eqrlkerivr tllvgmsihk ndetmmrngc lalcqfrlpq dvmsnykalv
      481 elllhsakha eqesyvqrig ifllntlacr vtgrekrllg nlgciktmle liryrvesgt
      541 fddvlevaws tmwnvtdetp vncqrfffed gmslflkcvk qyphkeellk nmmgllgnva
      601 evqylrvhfm qqpymnvfan llltvsegie vpynavgila hiasdgpeaw tiekpsrnev
      661 lelmveaier wdissernin yrsflpllrl ldiyhtpqcq hwavwalanl ttvyphkyci
      721 lvirenglek letlmsdprp yerikelahi vvencchysn ntsdlnahsp ldgdynldd
//
LOCUS       XP_034195729             702 aa            linear   INV 03-SEP-2025
DEFINITION  guanylate cyclase 1 soluble subunit alpha 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195729
VERSION     XP_034195729.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339838.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..702
                     /product="guanylate cyclase 1 soluble subunit alpha 2"
                     /calculated_mol_wt=78915
     Region          <116..235
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          287..479
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          458..648
                     /region_name="CYCc"
                     /note="Adenylyl- / guanylyl cyclase, catalytic domain;
                     smart00044"
                     /db_xref="CDD:214485"
     Site            order(497,499..504,539,541..543,546,608..610,614..615,
                     618..619)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(499,543)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(504,516,519..520,523,527,539..540,596,599,609..612,
                     615)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..702
                     /gene="Gycalpha99B"
                     /coded_by="XM_034339838.2:865..2973"
                     /db_xref="GeneID:117611704"
ORIGIN      
        1 macpfsrerl kasceeqrer gndgqprigr lgrddggksr qssfevtsll mreetddted
       61 tqtlnlkhlr aavlvltnps nevvavalga llskgeepls tsrsleklly nvdaeenynl
      121 lediyetlsy ecdvevstff dhlgqeliyt acvglleraf rclgndltaf lttldgvndv
      181 vqhqsgseae aefvciatpe aielhfttdh psiayllvgs lkgiarqfyn dnanvyilpd
      241 pyntkffryr itperyseql lvdsevddnv dtvtspfrpl steatdlrmg vasfckafpw
      301 hfvvdrqlel vqlgvgfmri fghhlnrlgr eistyfvftr psgvtltfhe ilkrantpfv
      361 ltlqrpqgvd kypaeglemk gqmvhcpesd silfvsspfl nglegltgrg lfisdiplhd
      421 atrdvilvge qaraqdglrr rmdklkssie eanhavsaer eknvsllhli fppdiakrlw
      481 lgetieakty pevtmlfsdi vgfteicsta tpmmvinmlq nlyeqfdsfc gqldvykvet
      541 igdaycvacg lhrdtcihaq qiawmalkmi qacsnhlthk gkpirmrigi htgmvlagvv
      601 gkkmpryclf ghnvtlankf eslseplrih vspttydllk ypvpgfelep rrkeflpkef
      661 paniegtsyf lygykhkdvd vnhpldlhiq saireygiga sl
//
LOCUS       XP_034195425             278 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611570 [Osmia lignaria lignaria].
ACCESSION   XP_034195425
VERSION     XP_034195425.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339534.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="uncharacterized protein LOC117611570"
                     /calculated_mol_wt=31694
     Region          74..233
                     /region_name="DUF4779"
                     /note="Domain of unknown function (DUF4779); pfam16009"
                     /db_xref="CDD:464971"
     CDS             1..278
                     /gene="LOC117611570"
                     /coded_by="XM_034339534.2:696..1532"
                     /db_xref="GeneID:117611570"
ORIGIN      
        1 mlravlpivl lafllqtyqa relnvprvrr dvriedndas qtvlseissa stkvgdskdd
       61 lvtaasghqg hrhesgggqs hhsdhheehg eedekgyksh hhhdkndkge hdkqhraghh
      121 eehgghkkah hdehekygeh hegekghkgg kfgekkghkk ghktkgyhnk fhkdeyhkeh
      181 kfyddyhkgg hhkkhgdfhg hhekkegqhk kgghhhsgyh edhhgkkghh dkghvdeehk
      241 ghhgkhghde hyshhdsygk kgdqhagkkf gyskghkg
//
LOCUS       XP_034195540             263 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611633 [Osmia lignaria lignaria].
ACCESSION   XP_034195540
VERSION     XP_034195540.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339649.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="uncharacterized protein LOC117611633"
                     /calculated_mol_wt=29872
     Region          62..204
                     /region_name="DUF4779"
                     /note="Domain of unknown function (DUF4779); pfam16009"
                     /db_xref="CDD:464971"
     CDS             1..263
                     /gene="LOC117611633"
                     /coded_by="XM_034339649.2:488..1279"
                     /db_xref="GeneID:117611633"
ORIGIN      
        1 makslclwla lamvcawlsl ttareirnem dlemaashhh ghhheegggh hhhshheddh
       61 gekgekgykh hhhhdkgdhg hygkehdegh heehgghkkg hhdeheehgh hhehekghkg
      121 ekhghkkghk kgekthgyhh kahkdeyhke hkfyddyhkg ghhekhgnhh ghheskdghh
      181 kkgghhhsgh hedhhgkkgh sdkghydddh kgyhgkhghd ehhshyedyg kkgehhegkk
      241 hgyssggggg gghggggghh hhh
//
LOCUS       XP_034195520             246 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611618 [Osmia lignaria lignaria].
ACCESSION   XP_034195520
VERSION     XP_034195520.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339629.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..246
                     /product="uncharacterized protein LOC117611618"
                     /calculated_mol_wt=28654
     Region          67..194
                     /region_name="DUF4779"
                     /note="Domain of unknown function (DUF4779); pfam16009"
                     /db_xref="CDD:464971"
     CDS             1..246
                     /gene="LOC117611618"
                     /coded_by="XM_034339629.1:200..940"
                     /db_xref="GeneID:117611618"
ORIGIN      
        1 mkfrlpicll lvclacqcli dareiredyd dlavaashhh ehhehgggde hhsdhhhkhg
       61 dhgdkgykss hhhekgdkgh hdkdehkghy deeeghkksh hhedgyydeh hkgekgekgh
      121 kfheeghygk ghktkghhev hkldefkkdk effdehhdsg hhedhgghhh ehehekgghf
      181 kkghhdhgeh ddhygkkghh ekghhhhdhk ghksddghde hhhhhshhgk kgghddhksw
      241 gfkkgh
//
LOCUS       XP_034195586             267 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611659 [Osmia lignaria lignaria].
ACCESSION   XP_034195586
VERSION     XP_034195586.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339695.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..267
                     /product="uncharacterized protein LOC117611659"
                     /calculated_mol_wt=29752
     Region          31..83
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..267
                     /gene="LOC117611659"
                     /coded_by="XM_034339695.1:112..915"
                     /db_xref="GeneID:117611659"
ORIGIN      
        1 magpylsplg pqadllteym fgrrrqrrnr ttftpqqlqe leslfqkthy pdvflreeva
       61 lrislsearv qvwfqnrrak wrkqarlqll qdawrmrclg lgsaaplllr pppttslerp
      121 daaspvpppp ppppppppst sattngspla sisankmsdt palsvcrdyr ilppphgysl
      181 scekspdrmk cgcgsppric gspverdtsl pmrdrpqeae mdltvkgtpr htpistlfcq
      241 lpqqqefsps qnvdepldvt lntnknn
//
LOCUS       XP_034195468            1922 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611587 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195468
VERSION     XP_034195468.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339577.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195468.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1922
                     /product="uncharacterized protein LOC117611587 isoform X1"
                     /calculated_mol_wt=215680
     Region          103..155
                     /region_name="HEAT_EZ"
                     /note="HEAT-like repeat; pfam13513"
                     /db_xref="CDD:463906"
     Region          129..155
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1702..1730
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(1719..1720,1723,1726..1727,1730,1757,1762,1765,
                     1768..1769,1774,1802..1803,1806,1809..1810,1838..1839,
                     1842,1845..1846)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          1739..1775
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1784..1810
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1821..1847
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1922
                     /gene="LOC117611587"
                     /coded_by="XM_034339577.2:669..6437"
                     /db_xref="GeneID:117611587"
ORIGIN      
        1 msecfefsss mtgstnsgpl qpqkdssplp sspfhfhstp kspsrrnhrq rlprkmtlts
       61 sngqpiqltp lwehvvrtrk fpsevdtrlt fleiserlrd pewevrqhal rvlidvlptl
      121 nadivdkvmq pvvpelvnnl ghpapavrkg aldalrvflt hnrdkentik rilqdglnrs
      181 evcdsfqtnv ttgvilsvps llfpssssps pspqlvkdat ialasrlaqv phqeavlksl
      241 tkirdavgte tfesyledyd nklkknieil skiynvrsnk rtqkksiekv teskeqdrkw
      301 dsdsdtsgia eeedevntga msaarrvvle teikfneeta itmtileekd dsekeqdees
      361 gtdvrndteg knashdrrkt prrvhfggei vklrtpdsde tesietipkt ripvpvspat
      421 kmpnarprps sqpcsprrea gktnrpsrsv ssspkrdvyt hnaelspkks ilartnspvs
      481 mkksaeqsrk kkntkngkqn petneeriai sgkpeeakre dvilvqnaqs sndqnsvivv
      541 keyenkdnnk aerieeevre eriledkrvt veniqkvdsl fgspircnet eqirddvned
      601 drsggklksc lttgskhdnv dcertsrgdr dkaapsviek ngnaggeaev kseepswegl
      661 glvdqevled lhnkedwrar vrglervasa lrtssaliai eprlgsllha vlgcerscrv
      721 aaaglavakv vvagvseeal kkqlpqlawg larhggpsat qlaritmlrl rpallleqll
      781 qpqclnarna ktrentlqll ifslvtfpst efkadiiank vakmvrdrrr rvrqaaldtl
      841 avlaqiyese evlaagkqas egyhdaeamm sairarlark slplvsvdgl vmyglqispt
      901 vqiatgpdvd wivagsgsvs psigrtksqi iatrsekerf arnenances pwierpnfva
      961 lgvgmrsktd pplvwqmvpt qnqnlgcede vnlletkgat tnirngeafn ldiksrnqtr
     1021 ipeypresan yriivedkfd rrdngnktes ripvlyirdr gakidynqdk sinsqlinpn
     1081 skrrfrdnie enfrnceamy yrrrknqqdy dnyrsmknna saeysdvadn snqelsnvns
     1141 rmypkyterd nlskksdrsk cscsssiesh qpslsknvqq sfimhnmyna vshrenrfgd
     1201 ensfrplqta ptpltypynt adfnqrtden ndsnlsrpkf hrrtkdaaaq kitnvevsss
     1261 daqtseyfpa icsgtpcrrr lrslspsqly hrqqfvkiyd tdktvneeey neknkqhfls
     1321 eenyrvaqmn vetqfegddy ehsssdaldn nqedieqdvq dtqedtdngs sssdrqnaes
     1381 afdiitgqss pardtvdfvi tldqntettd eviitsnede pskewssdee etlqnekqts
     1441 srqlefhddd qnesqlsssd eaertnikrr sivsvlsser ilaneslqts neilhsnfqn
     1501 vgasplrssa spkkssrsss rnsndfealk spgrgscsss rklsfeledk nfvdsgeekv
     1561 vasemqcekd syqtiesgqs rrgsktgesp tiiiasrphs stdvsnysen hvettvqiae
     1621 nnnnplrfvn esnennnley gqskdssset ntepgilkie tqpvesinls krlpskvpra
     1681 tgryrgissk rnsdkpektk pivqqcfahl eskdweitmk glktlsqlak qqpeyldvca
     1741 taticrllgr qikslrsqva rtaclaagdi fsssirgidq dfediaepll hrtadtnrfl
     1801 radsnaaldl miehlpphkt iavivqrgas hqnaivraat arllasivdr igpehtmilp
     1861 kdvkdkllst garllidgnl darnhakkmf rrimgcegfr kaltdavpet tlrhidktlk
     1921 tl
//
LOCUS       XP_034195469            1922 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611587 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195469
VERSION     XP_034195469.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339578.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195469.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1922
                     /product="uncharacterized protein LOC117611587 isoform X1"
                     /calculated_mol_wt=215680
     Region          103..155
                     /region_name="HEAT_EZ"
                     /note="HEAT-like repeat; pfam13513"
                     /db_xref="CDD:463906"
     Region          129..155
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1702..1730
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(1719..1720,1723,1726..1727,1730,1757,1762,1765,
                     1768..1769,1774,1802..1803,1806,1809..1810,1838..1839,
                     1842,1845..1846)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          1739..1775
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1784..1810
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1821..1847
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1922
                     /gene="LOC117611587"
                     /coded_by="XM_034339578.2:486..6254"
                     /db_xref="GeneID:117611587"
ORIGIN      
        1 msecfefsss mtgstnsgpl qpqkdssplp sspfhfhstp kspsrrnhrq rlprkmtlts
       61 sngqpiqltp lwehvvrtrk fpsevdtrlt fleiserlrd pewevrqhal rvlidvlptl
      121 nadivdkvmq pvvpelvnnl ghpapavrkg aldalrvflt hnrdkentik rilqdglnrs
      181 evcdsfqtnv ttgvilsvps llfpssssps pspqlvkdat ialasrlaqv phqeavlksl
      241 tkirdavgte tfesyledyd nklkknieil skiynvrsnk rtqkksiekv teskeqdrkw
      301 dsdsdtsgia eeedevntga msaarrvvle teikfneeta itmtileekd dsekeqdees
      361 gtdvrndteg knashdrrkt prrvhfggei vklrtpdsde tesietipkt ripvpvspat
      421 kmpnarprps sqpcsprrea gktnrpsrsv ssspkrdvyt hnaelspkks ilartnspvs
      481 mkksaeqsrk kkntkngkqn petneeriai sgkpeeakre dvilvqnaqs sndqnsvivv
      541 keyenkdnnk aerieeevre eriledkrvt veniqkvdsl fgspircnet eqirddvned
      601 drsggklksc lttgskhdnv dcertsrgdr dkaapsviek ngnaggeaev kseepswegl
      661 glvdqevled lhnkedwrar vrglervasa lrtssaliai eprlgsllha vlgcerscrv
      721 aaaglavakv vvagvseeal kkqlpqlawg larhggpsat qlaritmlrl rpallleqll
      781 qpqclnarna ktrentlqll ifslvtfpst efkadiiank vakmvrdrrr rvrqaaldtl
      841 avlaqiyese evlaagkqas egyhdaeamm sairarlark slplvsvdgl vmyglqispt
      901 vqiatgpdvd wivagsgsvs psigrtksqi iatrsekerf arnenances pwierpnfva
      961 lgvgmrsktd pplvwqmvpt qnqnlgcede vnlletkgat tnirngeafn ldiksrnqtr
     1021 ipeypresan yriivedkfd rrdngnktes ripvlyirdr gakidynqdk sinsqlinpn
     1081 skrrfrdnie enfrnceamy yrrrknqqdy dnyrsmknna saeysdvadn snqelsnvns
     1141 rmypkyterd nlskksdrsk cscsssiesh qpslsknvqq sfimhnmyna vshrenrfgd
     1201 ensfrplqta ptpltypynt adfnqrtden ndsnlsrpkf hrrtkdaaaq kitnvevsss
     1261 daqtseyfpa icsgtpcrrr lrslspsqly hrqqfvkiyd tdktvneeey neknkqhfls
     1321 eenyrvaqmn vetqfegddy ehsssdaldn nqedieqdvq dtqedtdngs sssdrqnaes
     1381 afdiitgqss pardtvdfvi tldqntettd eviitsnede pskewssdee etlqnekqts
     1441 srqlefhddd qnesqlsssd eaertnikrr sivsvlsser ilaneslqts neilhsnfqn
     1501 vgasplrssa spkkssrsss rnsndfealk spgrgscsss rklsfeledk nfvdsgeekv
     1561 vasemqcekd syqtiesgqs rrgsktgesp tiiiasrphs stdvsnysen hvettvqiae
     1621 nnnnplrfvn esnennnley gqskdssset ntepgilkie tqpvesinls krlpskvpra
     1681 tgryrgissk rnsdkpektk pivqqcfahl eskdweitmk glktlsqlak qqpeyldvca
     1741 taticrllgr qikslrsqva rtaclaagdi fsssirgidq dfediaepll hrtadtnrfl
     1801 radsnaaldl miehlpphkt iavivqrgas hqnaivraat arllasivdr igpehtmilp
     1861 kdvkdkllst garllidgnl darnhakkmf rrimgcegfr kaltdavpet tlrhidktlk
     1921 tl
//
LOCUS       XP_034195470            1839 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611587 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195470
VERSION     XP_034195470.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339579.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195470.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1839
                     /product="uncharacterized protein LOC117611587 isoform X2"
                     /calculated_mol_wt=205636
     Region          103..155
                     /region_name="HEAT_EZ"
                     /note="HEAT-like repeat; pfam13513"
                     /db_xref="CDD:463906"
     Region          129..155
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1619..1647
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(1636..1637,1640,1643..1644,1647,1674,1679,1682,
                     1685..1686,1691,1719..1720,1723,1726..1727,1755..1756,
                     1759,1762..1763)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          1656..1692
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1701..1727
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1738..1764
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1839
                     /gene="LOC117611587"
                     /coded_by="XM_034339579.2:671..6190"
                     /db_xref="GeneID:117611587"
ORIGIN      
        1 msecfefsss mtgstnsgpl qpqkdssplp sspfhfhstp kspsrrnhrq rlprkmtlts
       61 sngqpiqltp lwehvvrtrk fpsevdtrlt fleiserlrd pewevrqhal rvlidvlptl
      121 nadivdkvmq pvvpelvnnl ghpapavrkg aldalrvflt hnrdkentik rilqdglnrs
      181 evcdsfqtnv ttgvilsvps llfpssssps pspqlvkdat ialasrlaqv phqeavlksl
      241 tkirdavgte tfesyledyd nklkknieil skiynvrsnk rtqkksiekv teskeqdrkw
      301 dsdsdtsgia eeedevntga msaarrvvle teikfneeta itmtileekd dsekeqdees
      361 gtdvrndteg knashdrrkt prrvhfggei vklrtpdsde tesietipkt ripvpvspat
      421 kmpnarprps sqpcsprrea gktnrpsrsv ssspkrdvyt hnaelspkks ilartnspvs
      481 mkksaeqsrk kkntkngkqn petneeriai sgkpeeakre dvilvqnaqs sndqnsvivv
      541 keyenkdnnk aerieeevre eriledkrvt veniqkvdsl fgspircnet eqirddvned
      601 drsggklksc lttgskhdnv dcertsrgdr dkaapsviek ngnaggeaev kseepswegl
      661 glvdqevled lhnkedwrar vrglervasa lrtssaliai eprlgsllha vlgcerscrv
      721 aaaglavakv vvagvseeal kkqlpqlawg larhggpsat qlaritmlrl rpallleqll
      781 qpqclnarna ktrentlqll ifslvtfpst efkadiiank vakmvrdrrr rvrqaaldtl
      841 avlaqiyese evlaagkqas egyhdaeamm sairarlark slplvsvdgl vmyglqispt
      901 vqiatgpdvd wivagsgsvs psigrtksqi iatrsekerf arnenances pwierpnfva
      961 lgvgmrsktd pplvwqmvpt qnqnlgcede vnlletkgat tnirngeafn ldiksrnqtr
     1021 ipeypresan yriivedkfd rrdvadnsnq elsnvnsrmy pkyterdnls kksdrskcsc
     1081 sssieshqps lsknvqqsfi mhnmynavsh renrfgdens frplqtaptp ltypyntadf
     1141 nqrtdennds nlsrpkfhrr tkdaaaqkit nvevsssdaq tseyfpaics gtpcrrrlrs
     1201 lspsqlyhrq qfvkiydtdk tvneeeynek nkqhflseen yrvaqmnvet qfegddyehs
     1261 ssdaldnnqe dieqdvqdtq edtdngssss drqnaesafd iitgqsspar dtvdfvitld
     1321 qntettdevi itsnedepsk ewssdeeetl qnekqtssrq lefhdddqne sqlsssdeae
     1381 rtnikrrsiv svlsserila neslqtsnei lhsnfqnvga splrssaspk kssrsssrns
     1441 ndfealkspg rgscsssrkl sfeledknfv dsgeekvvas emqcekdsyq tiesgqsrrg
     1501 sktgesptii iasrphsstd vsnysenhve ttvqiaennn nplrfvnesn ennnleygqs
     1561 kdsssetnte pgilkietqp vesinlskrl pskvpratgr yrgisskrns dkpektkpiv
     1621 qqcfahlesk dweitmkglk tlsqlakqqp eyldvcatat icrllgrqik slrsqvarta
     1681 claagdifss sirgidqdfe diaepllhrt adtnrflrad snaaldlmie hlpphktiav
     1741 ivqrgashqn aivraatarl lasivdrigp ehtmilpkdv kdkllstgar llidgnldar
     1801 nhakkmfrri mgcegfrkal tdavpettlr hidktlktl
//
LOCUS       XP_034195471            1000 aa            linear   INV 03-SEP-2025
DEFINITION  cilia- and flagella-associated protein 70 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195471
VERSION     XP_034195471.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339580.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195471.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1000
                     /product="cilia- and flagella-associated protein 70"
                     /calculated_mol_wt=116148
     Region          595..690
                     /region_name="NrfG"
                     /note="Cytochrome c-type biogenesis protein CcmH/NrfG
                     [Energy production and conversion, Posttranslational
                     modification, protein turnover, chaperones]; COG4235"
                     /db_xref="CDD:443378"
     Region          596..624
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(597,600..601,604..605,607,631,634..635,638..639,
                     641..642,665,668..669,672..673,676)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          629..659
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          664..690
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          754..780
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          788..>949
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          788..814
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(790..791,794..795,797,821,824..825,828..829,
                     831..832,855,858,862,865..866,869)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          819..849
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          854..880
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          892..918
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(894..895,898..899,901,925,928..929,932..933,
                     935..936,959,962..963,966..967,970)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          923..953
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..1000
                     /gene="LOC117611588"
                     /coded_by="XM_034339580.2:133..3135"
                     /db_xref="GeneID:117611588"
ORIGIN      
        1 mekeqnlseg knieitidti qnvikktnvs vsfivehngt ilgnsgptfi sancedvvyg
       61 vefvvslpvs tkdkqnlnsi vstpvlikam qnvesehdek sigsekgtkk ksvftsksis
      121 tggsamlgic nfdllpiilg ekcyteklil etpkffmdea lvswqnlpll tvtasqnnvp
      181 ffqpeesvnf lnitvesiyn lpqtftenlq ykvgtiayid neipeniife nglwtkfrdv
      241 ertkrwnslr flenraqlsk ykldcdfmgv knefkkwfnl tdkvyedlpr iewnslerfi
      301 twttgieamk nhiikyrywp fqimitekgv psklsmllyq cyidlsellf sgrkscrvvg
      361 qlysynptdi sekvglekni fdlekgpkes kerekkskas khspsetaet vfepsvelis
      421 enaeptvivi evelykplfv stviedftri ydmiskpekk tryvytsela ekqfmnciqk
      481 lteyitecyr dfreeneqse eskekycfnp sedelacftq hlyktgqyla irntlrskvp
      541 llldqkfnmp rnfvdsnetq nfiasiysyl veqmhialnk ivenrftddl dhsvdsklmy
      601 fyaeeayelg eldraryyyt tviasnksdp hswtkyamfl kkigdverak eccleaisld
      661 rhhpigllmy alllfenrey keaetflral tnfyprffec waslyifyir teyypgidlt
      721 lriaekcikd ktkqiildqe slswtmvhcp qdnvciliat fllklhfcef agialaqams
      781 nsnqstsvly yiavehylsg rfedalshlk eaecnygidf sisslmghcy lklgedekav
      841 eyydfacmlf drprmahlve irlgyhhynv nefdrakrlf lntcktspal htwlgvgmsc
      901 yelghfeeae malmeaniid nrnadtwgyl cllnmtlery defsqcyaem iknnlknkkl
      961 wlritnlmka ldytspdlat gtndfitsqn eeesqeqfeq
//
LOCUS       XP_034195473             930 aa            linear   INV 03-SEP-2025
DEFINITION  periodic tryptophan protein 2 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034195473
VERSION     XP_034195473.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339582.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195473.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..930
                     /product="periodic tryptophan protein 2 homolog"
                     /calculated_mol_wt=104473
     Region          10..468
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          19..52
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          58..94
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          99..138
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          149..185
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          314..660
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          356..394
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          400..436
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          441..477
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          485..521
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          527..560
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          571..609
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          623..668
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          796..898
                     /region_name="Utp12"
                     /note="Dip2/Utp12 Family; pfam04003"
                     /db_xref="CDD:461125"
     CDS             1..930
                     /gene="LOC117611591"
                     /coded_by="XM_034339582.2:103..2895"
                     /db_xref="GeneID:117611591"
ORIGIN      
        1 mkfaykfsnl lgavyrkgnl ifspdgstvi spvgnritiy dlknnksstl piestynytc
       61 mdlspngtll iavnevgegn iismiskmvi hkyhfkqrvr cvkfspdgkh favckehkvf
      121 ifnapnlysc eynpfimehv flgavddttc idwsydskli avgskdmttk iygiekfanf
      181 ryislgshsd aivacffekk tydlsticrn grlciwecti npedlvqwep rskrdkheds
      241 dieddidvek viektdkqak ayehslqqsq naietendkn sdndteikkl ryqklcrhyl
      301 tnevqkerke ivkltaaayh qethilvvgf sngsfylyem pevnmihsls isqqyissia
      361 instgdwial gcstagqllv wewqsetyam kqqghsnnmn clayspdgqy ivtggddgkv
      421 klwnttngfc sitfqehtst vtgvlfshnr kfivstsldg tvraydlary rnfktltspr
      481 pvqfscvald ssdeflaagg qdffeiylws vklgtlleil sghegpvvsl afnpniaste
      541 lvsvswdksl kiwnavengs shetiqltsd glyvtykpng eevavatldg hisffdckta
      601 vqtgsiegrn dlgsgrsetd litakkslqg kaftslcysa dgtcilaggq skniciynvq
      661 ecmlvkkfvv tqnrsldavd eyinkrklte fgskalieer eeneggnvtl rlpgvksgdm
      721 asrsikpevr vfslqfsptg qawaaatteg lliysldvgl vfdpfqlelg itpdtvkial
      781 neneyakalm malrlnekll iqrvvenipl sdielttanl pdiyvdkvlk fiaseleltr
      841 hvhfyllwie tiltkhgaki nsalqmpill tlhknmqkky ddlskicdfn qytmsylkkv
      901 gefkakkpmn veinsdeese kssieemdvd
//
LOCUS       XP_034195474             797 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611592 [Osmia lignaria lignaria].
ACCESSION   XP_034195474
VERSION     XP_034195474.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339583.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195474.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..797
                     /product="uncharacterized protein LOC117611592"
                     /calculated_mol_wt=91028
     Region          <163..>196
                     /region_name="DUF4594"
                     /note="Domain of unknown function (DUF4594); pfam15266"
                     /db_xref="CDD:464604"
     Region          <352..647
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..797
                     /gene="LOC117611592"
                     /coded_by="XM_034339583.2:247..2640"
                     /db_xref="GeneID:117611592"
ORIGIN      
        1 maanalteki nkirqlneei krrheeveed kknaakqnal vqmvpstdwp erkeppefti
       61 spktkqkstk erheyipqsh sgegrkihsf aqgegpppdp kynfladser eepmeyaket
      121 vgsrstnkgs rgsfrkkigg renvekdnki nkgnykdesq sgydawraer nridedrisr
      181 qrtaegnwlr ewdndkfish srndeferdp nrehaptqyy sdrlenttfp tfsrikkkyk
      241 dsgrrydnnn ehvnynqsgn prpyrgsakn vhgnrdnrsh ltynqhrnnv sasakvaesl
      301 tseertviat dksikvtvnq ssatkgpvms ikvnspsiag tgrvgprqrt rvtyshsdid
      361 tpmtenesff rqksfedksk gtffnnnqkf sninrshsqr ksdsrplhyq rkegrkednd
      421 tnfhkyheae frsysqkdqq ksyvakspkp mrgntkiiks essnvnvsqk tvaqeslttd
      481 etnvehdveh dvktensesv qlteadhtei staetseini enktindiil kkeseetsln
      541 lssntenteh vdkdngnkdl eevantstkd lpetrdsnvn ktdfnnekks seevtnhlte
      601 dvqenndsns inkndsdner vnsekiannl penndtssei nnaqfkevlv lnennevsdd
      661 ivksqevfsp kddqqpleqv vkqfvhadck dtedqvstqq tvevatdnsv slletntqsv
      721 ievndqlacn sevvqhqeek tnennessde ilkdvncset npedtisnmn isinsteekk
      781 ekseeiingk vedaeel
//
LOCUS       XP_034195477             779 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil and C2 domain-containing protein 2A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195477
VERSION     XP_034195477.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339586.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195477.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..779
                     /product="coiled-coil and C2 domain-containing protein 2A
                     isoform X1"
                     /calculated_mol_wt=88642
     Region          327..426
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     CDS             1..779
                     /gene="LOC117611593"
                     /coded_by="XM_034339586.2:615..2954"
                     /db_xref="GeneID:117611593"
ORIGIN      
        1 mstldidfik gyaqrienqt eenlslcysy ptihqdqgtk vlttdnrslq klnqetnlve
       61 dglyasdspf iyndikssef snmkmleyes nhkfhgklle ivirdvnint lrdqsdtvei
      121 tsvcllfkkk iickanspfn rklhkllirn mecnnlsiqv ltkggqssil plplpkrnve
      181 nnkveidfai sdnlgkihag tvistvtvkn ygkqkeepct sqlyklsndp ndpqnslsll
      241 rppknlckkq vkyflledpa ltfnvdsevt vskrvqeenv kppdivvteq palslldmsf
      301 rnlfqikhpl esssgtrrhh tigktvlavt ilrgieipir eesalvqpfi evewgdtiha
      361 tsiaegpapv wqqtvqfelp rqsgeycikf rlydqhpvwg qqwlgetrip leyhrnyqel
      421 erwitlsplf spvllfgyiq aspgqsctri yvlikmehpn tpksvesttt ntllkgiqrc
      481 lvtsykisgl enpkdaakiv mllpslpahy gpitprqaln ikkvdhygra allavllqsf
      541 dlqtyvllgs sqiskltafv lsisnngiyv lwdpetgnyh klddshcplm kvsrlinyfg
      601 iwenlqksil phnlkydvks skdwrsvdtt tlvktdhnvq tlelqitdee vkqikktaid
      661 ieqclkdkla dwrsnigltt ifnrhatavl rnsiskiepf seiqlgkkdl kqlyrayhih
      721 gfvlnkrech vdelckdlya tkihnisgpv efavvchiqc yagkissiwl aiiilrshd
//
LOCUS       XP_034195476             779 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil and C2 domain-containing protein 2A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195476
VERSION     XP_034195476.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339585.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195476.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..779
                     /product="coiled-coil and C2 domain-containing protein 2A
                     isoform X1"
                     /calculated_mol_wt=88642
     Region          327..426
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     CDS             1..779
                     /gene="LOC117611593"
                     /coded_by="XM_034339585.2:622..2961"
                     /db_xref="GeneID:117611593"
ORIGIN      
        1 mstldidfik gyaqrienqt eenlslcysy ptihqdqgtk vlttdnrslq klnqetnlve
       61 dglyasdspf iyndikssef snmkmleyes nhkfhgklle ivirdvnint lrdqsdtvei
      121 tsvcllfkkk iickanspfn rklhkllirn mecnnlsiqv ltkggqssil plplpkrnve
      181 nnkveidfai sdnlgkihag tvistvtvkn ygkqkeepct sqlyklsndp ndpqnslsll
      241 rppknlckkq vkyflledpa ltfnvdsevt vskrvqeenv kppdivvteq palslldmsf
      301 rnlfqikhpl esssgtrrhh tigktvlavt ilrgieipir eesalvqpfi evewgdtiha
      361 tsiaegpapv wqqtvqfelp rqsgeycikf rlydqhpvwg qqwlgetrip leyhrnyqel
      421 erwitlsplf spvllfgyiq aspgqsctri yvlikmehpn tpksvesttt ntllkgiqrc
      481 lvtsykisgl enpkdaakiv mllpslpahy gpitprqaln ikkvdhygra allavllqsf
      541 dlqtyvllgs sqiskltafv lsisnngiyv lwdpetgnyh klddshcplm kvsrlinyfg
      601 iwenlqksil phnlkydvks skdwrsvdtt tlvktdhnvq tlelqitdee vkqikktaid
      661 ieqclkdkla dwrsnigltt ifnrhatavl rnsiskiepf seiqlgkkdl kqlyrayhih
      721 gfvlnkrech vdelckdlya tkihnisgpv efavvchiqc yagkissiwl aiiilrshd
//
LOCUS       XP_034195478             779 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil and C2 domain-containing protein 2A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195478
VERSION     XP_034195478.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339587.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195478.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..779
                     /product="coiled-coil and C2 domain-containing protein 2A
                     isoform X1"
                     /calculated_mol_wt=88642
     Region          327..426
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     CDS             1..779
                     /gene="LOC117611593"
                     /coded_by="XM_034339587.2:688..3027"
                     /db_xref="GeneID:117611593"
ORIGIN      
        1 mstldidfik gyaqrienqt eenlslcysy ptihqdqgtk vlttdnrslq klnqetnlve
       61 dglyasdspf iyndikssef snmkmleyes nhkfhgklle ivirdvnint lrdqsdtvei
      121 tsvcllfkkk iickanspfn rklhkllirn mecnnlsiqv ltkggqssil plplpkrnve
      181 nnkveidfai sdnlgkihag tvistvtvkn ygkqkeepct sqlyklsndp ndpqnslsll
      241 rppknlckkq vkyflledpa ltfnvdsevt vskrvqeenv kppdivvteq palslldmsf
      301 rnlfqikhpl esssgtrrhh tigktvlavt ilrgieipir eesalvqpfi evewgdtiha
      361 tsiaegpapv wqqtvqfelp rqsgeycikf rlydqhpvwg qqwlgetrip leyhrnyqel
      421 erwitlsplf spvllfgyiq aspgqsctri yvlikmehpn tpksvesttt ntllkgiqrc
      481 lvtsykisgl enpkdaakiv mllpslpahy gpitprqaln ikkvdhygra allavllqsf
      541 dlqtyvllgs sqiskltafv lsisnngiyv lwdpetgnyh klddshcplm kvsrlinyfg
      601 iwenlqksil phnlkydvks skdwrsvdtt tlvktdhnvq tlelqitdee vkqikktaid
      661 ieqclkdkla dwrsnigltt ifnrhatavl rnsiskiepf seiqlgkkdl kqlyrayhih
      721 gfvlnkrech vdelckdlya tkihnisgpv efavvchiqc yagkissiwl aiiilrshd
//
LOCUS       XP_034195479             779 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil and C2 domain-containing protein 2A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195479
VERSION     XP_034195479.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339588.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195479.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..779
                     /product="coiled-coil and C2 domain-containing protein 2A
                     isoform X1"
                     /calculated_mol_wt=88642
     Region          327..426
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     CDS             1..779
                     /gene="LOC117611593"
                     /coded_by="XM_034339588.2:572..2911"
                     /db_xref="GeneID:117611593"
ORIGIN      
        1 mstldidfik gyaqrienqt eenlslcysy ptihqdqgtk vlttdnrslq klnqetnlve
       61 dglyasdspf iyndikssef snmkmleyes nhkfhgklle ivirdvnint lrdqsdtvei
      121 tsvcllfkkk iickanspfn rklhkllirn mecnnlsiqv ltkggqssil plplpkrnve
      181 nnkveidfai sdnlgkihag tvistvtvkn ygkqkeepct sqlyklsndp ndpqnslsll
      241 rppknlckkq vkyflledpa ltfnvdsevt vskrvqeenv kppdivvteq palslldmsf
      301 rnlfqikhpl esssgtrrhh tigktvlavt ilrgieipir eesalvqpfi evewgdtiha
      361 tsiaegpapv wqqtvqfelp rqsgeycikf rlydqhpvwg qqwlgetrip leyhrnyqel
      421 erwitlsplf spvllfgyiq aspgqsctri yvlikmehpn tpksvesttt ntllkgiqrc
      481 lvtsykisgl enpkdaakiv mllpslpahy gpitprqaln ikkvdhygra allavllqsf
      541 dlqtyvllgs sqiskltafv lsisnngiyv lwdpetgnyh klddshcplm kvsrlinyfg
      601 iwenlqksil phnlkydvks skdwrsvdtt tlvktdhnvq tlelqitdee vkqikktaid
      661 ieqclkdkla dwrsnigltt ifnrhatavl rnsiskiepf seiqlgkkdl kqlyrayhih
      721 gfvlnkrech vdelckdlya tkihnisgpv efavvchiqc yagkissiwl aiiilrshd
//
LOCUS       XP_076546593             779 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil and C2 domain-containing protein 2A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546593
VERSION     XP_076546593.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690478.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..779
                     /product="coiled-coil and C2 domain-containing protein 2A
                     isoform X1"
                     /calculated_mol_wt=88642
     Region          327..426
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     CDS             1..779
                     /gene="LOC117611593"
                     /coded_by="XM_076690478.1:549..2888"
                     /db_xref="GeneID:117611593"
ORIGIN      
        1 mstldidfik gyaqrienqt eenlslcysy ptihqdqgtk vlttdnrslq klnqetnlve
       61 dglyasdspf iyndikssef snmkmleyes nhkfhgklle ivirdvnint lrdqsdtvei
      121 tsvcllfkkk iickanspfn rklhkllirn mecnnlsiqv ltkggqssil plplpkrnve
      181 nnkveidfai sdnlgkihag tvistvtvkn ygkqkeepct sqlyklsndp ndpqnslsll
      241 rppknlckkq vkyflledpa ltfnvdsevt vskrvqeenv kppdivvteq palslldmsf
      301 rnlfqikhpl esssgtrrhh tigktvlavt ilrgieipir eesalvqpfi evewgdtiha
      361 tsiaegpapv wqqtvqfelp rqsgeycikf rlydqhpvwg qqwlgetrip leyhrnyqel
      421 erwitlsplf spvllfgyiq aspgqsctri yvlikmehpn tpksvesttt ntllkgiqrc
      481 lvtsykisgl enpkdaakiv mllpslpahy gpitprqaln ikkvdhygra allavllqsf
      541 dlqtyvllgs sqiskltafv lsisnngiyv lwdpetgnyh klddshcplm kvsrlinyfg
      601 iwenlqksil phnlkydvks skdwrsvdtt tlvktdhnvq tlelqitdee vkqikktaid
      661 ieqclkdkla dwrsnigltt ifnrhatavl rnsiskiepf seiqlgkkdl kqlyrayhih
      721 gfvlnkrech vdelckdlya tkihnisgpv efavvchiqc yagkissiwl aiiilrshd
//
LOCUS       XP_076546594             744 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil and C2 domain-containing protein 2A isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546594
VERSION     XP_076546594.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690479.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..744
                     /product="coiled-coil and C2 domain-containing protein 2A
                     isoform X2"
                     /calculated_mol_wt=84791
     Region          327..426
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     CDS             1..744
                     /gene="LOC117611593"
                     /coded_by="XM_076690479.1:613..2847"
                     /db_xref="GeneID:117611593"
ORIGIN      
        1 mstldidfik gyaqrienqt eenlslcysy ptihqdqgtk vlttdnrslq klnqetnlve
       61 dglyasdspf iyndikssef snmkmleyes nhkfhgklle ivirdvnint lrdqsdtvei
      121 tsvcllfkkk iickanspfn rklhkllirn mecnnlsiqv ltkggqssil plplpkrnve
      181 nnkveidfai sdnlgkihag tvistvtvkn ygkqkeepct sqlyklsndp ndpqnslsll
      241 rppknlckkq vkyflledpa ltfnvdsevt vskrvqeenv kppdivvteq palslldmsf
      301 rnlfqikhpl esssgtrrhh tigktvlavt ilrgieipir eesalvqpfi evewgdtiha
      361 tsiaegpapv wqqtvqfelp rqsgeycikf rlydqhpvwg qqwlgetrip leyhrnyqel
      421 erwitlsplf spvllfgyiq aspgqsctri yvlikmehpn tpksvesttt ntllkgiqrc
      481 lvtsykisgl enpkdaakiv mllpslpahy gpitprqaln ikkvdhygra allavllqsf
      541 dlqtyvllgs sqiskltafv lsisnngiyv lwdpetgnyh klddshcplm kvsrlinyfg
      601 iwenlqksil phnlkydvks skdwrsvdtt tlvktdhnvq tlelqitdee vkqikktaid
      661 ieqclkdkla dwrsnigltt ifnrhatavl rnsiskiepf seiqlvemfd eqmllginnt
      721 fffdiftlhs ndvfitiisy isrh
//
LOCUS       XP_076546595             708 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil and C2 domain-containing protein 2A isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546595
VERSION     XP_076546595.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690480.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="coiled-coil and C2 domain-containing protein 2A
                     isoform X3"
                     /calculated_mol_wt=80439
     Region          327..426
                     /region_name="C2"
                     /note="C2 domain; cd00030"
                     /db_xref="CDD:175973"
     CDS             1..708
                     /gene="LOC117611593"
                     /coded_by="XM_076690480.1:613..2739"
                     /db_xref="GeneID:117611593"
ORIGIN      
        1 mstldidfik gyaqrienqt eenlslcysy ptihqdqgtk vlttdnrslq klnqetnlve
       61 dglyasdspf iyndikssef snmkmleyes nhkfhgklle ivirdvnint lrdqsdtvei
      121 tsvcllfkkk iickanspfn rklhkllirn mecnnlsiqv ltkggqssil plplpkrnve
      181 nnkveidfai sdnlgkihag tvistvtvkn ygkqkeepct sqlyklsndp ndpqnslsll
      241 rppknlckkq vkyflledpa ltfnvdsevt vskrvqeenv kppdivvteq palslldmsf
      301 rnlfqikhpl esssgtrrhh tigktvlavt ilrgieipir eesalvqpfi evewgdtiha
      361 tsiaegpapv wqqtvqfelp rqsgeycikf rlydqhpvwg qqwlgetrip leyhrnyqel
      421 erwitlsplf spvllfgyiq aspgqsctri yvlikmehpn tpksvesttt ntllkgiqrc
      481 lvtsykisgl enpkdaakiv mllpslpahy gpitprqaln ikkvdhygra allavllqsf
      541 dlqtyvllgs sqiskltafv lsisnngiyv lwdpetgnyh klddshcplm kvsrlinyfg
      601 iwenlqksil phnlkydvks skdwrsvdtt tlvktdhnvq tlelqitdee vkqikktaid
      661 ieqclkdkla dwrsnigltt ifnrhatavl rnsiskiepf seiqlvae
//
LOCUS       XP_034195481             666 aa            linear   INV 03-SEP-2025
DEFINITION  TBC1 domain family member 23 [Osmia lignaria lignaria].
ACCESSION   XP_034195481
VERSION     XP_034195481.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339590.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..666
                     /product="TBC1 domain family member 23"
                     /calculated_mol_wt=74988
     Region          88..235
                     /region_name="RabGAP-TBC"
                     /note="Rab-GTPase-TBC domain; pfam00566"
                     /db_xref="CDD:459855"
     Region          325..>373
                     /region_name="Rhodanese"
                     /note="Rhodanese-like domain; pfam00581"
                     /db_xref="CDD:425764"
     Region          547..660
                     /region_name="TBC1D23_C-like"
                     /note="C-terminal domain of TBC1 domain family member 23,
                     and similar proteins; cd20788"
                     /db_xref="CDD:412053"
     CDS             1..666
                     /gene="TBC1D23"
                     /coded_by="XM_034339590.2:344..2344"
                     /db_xref="GeneID:117611594"
ORIGIN      
        1 malqedentw vleleaalld teapsasdiy aickgqavpt nlrpdvwqac ldvidrgnql
       61 nhfnevfdlp eqniirddcq qlvaklgndd edkvsvvsdl esiltfycks krkqyergng
      121 wiellgplia lklprsptyn lfeaiidiyi prgetyssvl rllllyhepe lcsfldtkrv
      181 spdqytkgwv ttlfagvcsl pavctmwdly fmqadpffml flslimvina reqilsmkdn
      241 dkqsiidaia alpcaleaed vtdfcslaqy yamktpssfk hdlypvmfge sfdekyisha
      301 lclpvsaqel vensiespsv pntsvesvrf flvdcrpaeq ynaghlptaf hldcnlmlqe
      361 paafatavqg llqaqrqala vgsqaggehl cflgsgrqee drythmvvas flqkhtqyvs
      421 mvtsgyqaih eyfgdevvss lidhnsqhcl vcnanisetn snetspvkvk nnnsdflgki
      481 kkvkgklfdy ivnpsasvhs nmenrnskdl eyskrqrktg pvfsidddqe ldmtmtnnes
      541 eepievvsiq qwmkdpkllh sfkceevkvn gdlcdsillv tdthlivlre iqerkgaahv
      601 ivkrpltsiv kitsrkkysd litfkygttq yndtvisdmd kflipnasea tklitqqilk
      661 qlktsn
//
LOCUS       XP_034195482             662 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 62 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195482
VERSION     XP_034195482.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339591.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..662
                     /product="transmembrane protein 62 isoform X1"
                     /calculated_mol_wt=76414
     Region          65..361
                     /region_name="MPP_TMEM62_N"
                     /note="Homo sapiens TMEM62, N-terminal metallophosphatase
                     domain; cd07401"
                     /db_xref="CDD:277346"
     Site            order(71,73,107,150..151,242,273,275)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:277346"
     Site            order(71,73,107,150,242,273,275)
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:277346"
     CDS             1..662
                     /gene="LOC117611595"
                     /coded_by="XM_034339591.2:341..2329"
                     /db_xref="GeneID:117611595"
ORIGIN      
        1 mkitkstvll lvfvlvlsil vanvadlinv dthvlddtsp nknvrndwsk pkfydigass
       61 dhliwflqis dihisifqdp lritelkefc nitvgtikpp vvlasgdltd aktkdkmgsk
      121 qileewqyyk rilddtevnk rtlwldvrgn hdnfnvvsle srenyyynys iqgkkhprsy
      181 mytinigtel ytfiaidacl kpgprrpfnf vgvldereir sihelmnksq esnadyimwf
      241 ghyptscils qsstgirnvi grykesmayl cghyhmlggi apnmytlqqa gfleleladw
      301 kdnriyrlga idhgqfsfid vkhkewpvvl itnpkhalym mprkenlmsi ikstyirila
      361 fsiapikive vrlddsawsk cehvngplyv lkwnsteysd gihtiraqvt dvdgretvvs
      421 qpfaldgsrl sfrilprlil msnvsvifqf lfgtvlvlvv iplcllrflh ilceknqmhr
      481 prfkikfiqa wlrklwilst vdrlffplvl ytlyltvgpw aigevienht gvifawgtfv
      541 knsylpggft yaygffqlfs fhlplmlilg hradkrlqta nklhdkqlsk icrlweylpi
      601 tllvlmqtcm ayffwlaygt lativcplrt wsiflavmlw hqvntmpqsc lrsaaeiwss
      661 hg
//
LOCUS       XP_034195483             634 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 62 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195483
VERSION     XP_034195483.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339592.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..634
                     /product="transmembrane protein 62 isoform X2"
                     /calculated_mol_wt=73336
     Region          65..333
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     Site            order(71,73,107,150..151,214,245)
                     /site_type="active"
                     /db_xref="CDD:277317"
     Site            order(71,73,107,150,214,245)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:277317"
     CDS             1..634
                     /gene="LOC117611595"
                     /coded_by="XM_034339592.2:341..2245"
                     /db_xref="GeneID:117611595"
ORIGIN      
        1 mkitkstvll lvfvlvlsil vanvadlinv dthvlddtsp nknvrndwsk pkfydigass
       61 dhliwflqis dihisifqdp lritelkefc nitvgtikpp vvlasgdltd aktkdkmgsk
      121 qileewqyyk rilddtevnk rtlwldvrgn hdnfnvvsle srenyyynys iqgkkhprsy
      181 mytinidere irsihelmnk sqesnadyim wfghyptsci lsqsstgirn vigrykesma
      241 ylcghyhmlg giapnmytlq qagflelela dwkdnriyrl gaidhgqfsf idvkhkewpv
      301 vlitnpkhal ymmprkenlm siikstyiri lafsiapiki vevrlddsaw skcehvngpl
      361 yvlkwnstey sdgihtiraq vtdvdgretv vsqpfaldgs rlsfrilprl ilmsnvsvif
      421 qflfgtvlvl vviplcllrf lhilceknqm hrprfkikfi qawlrklwil stvdrlffpl
      481 vlytlyltvg pwaigevien htgvifawgt fvknsylpgg ftyaygffql fsfhlplmli
      541 lghradkrlq tanklhdkql skicrlweyl pitllvlmqt cmayffwlay gtlativcpl
      601 rtwsiflavm lwhqvntmpq sclrsaaeiw sshg
//
LOCUS       XP_034195484             546 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 62 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195484
VERSION     XP_034195484.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339593.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="transmembrane protein 62 isoform X3"
                     /calculated_mol_wt=63484
     Region          <1..245
                     /region_name="MPP_superfamily"
                     /note="metallophosphatase superfamily, metallophosphatase
                     domain; cl13995"
                     /db_xref="CDD:472684"
     CDS             1..546
                     /gene="LOC117611595"
                     /coded_by="XM_034339593.2:161..1801"
                     /db_xref="GeneID:117611595"
ORIGIN      
        1 mgskqileew qyykrilddt evnkrtlwld vrgnhdnfnv vslesrenyy ynysiqgkkh
       61 prsymytini gtelytfiai daclkpgprr pfnfvgvlde reirsihelm nksqesnady
      121 imwfghypts cilsqsstgi rnvigrykes maylcghyhm lggiapnmyt lqqagflele
      181 ladwkdnriy rlgaidhgqf sfidvkhkew pvvlitnpkh alymmprken lmsiikstyi
      241 rilafsiapi kivevrldds awskcehvng plyvlkwnst eysdgihtir aqvtdvdgre
      301 tvvsqpfald gsrlsfrilp rlilmsnvsv ifqflfgtvl vlvviplcll rflhilcekn
      361 qmhrprfkik fiqawlrklw ilstvdrlff plvlytlylt vgpwaigevi enhtgvifaw
      421 gtfvknsylp ggftyaygff qlfsfhlplm lilghradkr lqtanklhdk qlskicrlwe
      481 ylpitllvlm qtcmayffwl aygtlativc plrtwsifla vmlwhqvntm pqsclrsaae
      541 iwsshg
//
LOCUS       XP_034195485             581 aa            linear   INV 03-SEP-2025
DEFINITION  translocase of outer membrane 70 [Osmia lignaria lignaria].
ACCESSION   XP_034195485
VERSION     XP_034195485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="translocase of outer membrane 70"
                     /calculated_mol_wt=65313
     Region          31..568
                     /region_name="3a0801s09"
                     /note="mitochondrial precursor proteins import receptor
                     (72 kDa mitochondrial outermembrane protein)
                     (mitochondrial import receptor for the ADP/ATP carrier)
                     (translocase of outermembrane tom70); TIGR00990"
                     /db_xref="CDD:273380"
     Region          91..119
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(92,95..96,99..100,102,131,134..135,138..139,
                     141..142,165,168..169,172..173,176)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          124..159
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          164..190
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          298..325
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          335..363
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          370..397
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          402..439
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          444..472
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(445,448..449,452..453,455,479,482..483,486..487,
                     489..490,514,517..518,521..522,525)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          477..508
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          513..541
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..581
                     /gene="Tom70"
                     /coded_by="XM_034339594.2:185..1930"
                     /db_xref="GeneID:117611597"
ORIGIN      
        1 mtgasgassv tssplpkwql alavaapval glgymyyknn tnnkpssksn rgkskgsske
       61 ngtpatdkqi sididyppks tsepetllek aqkyknegne qfkkgkydea iiqynlaiei
      121 cpkentevla tfyqnraaay eqlkkysavk adctkalelk pkyakallrr aramehcndl
      181 esaledvtaa cilenfsnqt tilmadrvlk qlgrqhameh lankklimps kyfiktyist
      241 fpkdpvfvrl qnandgnisq gllkvlesvk eqnyddvipl cneeldssep ntllhrmevl
      301 ltratfylll gqhdaaiedl gtiidndsvs ngvkvnalik ratlfmqlen aeksfcdfkm
      361 aaeldpecgd iyhhrgqvnl lmenideare dfrkavelnp nfgvafvqkc yadyrygivk
      421 rdmeviream kgfeqavekf pdcpecytly aqmlsetqdy qkadryfaka iekdptnatv
      481 hvhrgllqlq wnadvdkave yinkallldd kceygyetlg tievqrgnlt eaiklfdkal
      541 algrtamelt hifslrdaak tqltikdklg ssimfnfqnv s
//
LOCUS       XP_034195486             512 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase RNF14 [Osmia lignaria lignaria].
ACCESSION   XP_034195486
VERSION     XP_034195486.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339595.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195486.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="E3 ubiquitin-protein ligase RNF14"
                     /calculated_mol_wt=60267
     Region          6..132
                     /region_name="RWD_RNF14"
                     /note="RWD domain of RING finger protein 14 (RNF14) and
                     related proteins; cd23820"
                     /db_xref="CDD:467656"
     Region          230..288
                     /region_name="RING-HC_RBR_RNF14"
                     /note="RING finger, HC subclass, found in RING finger
                     protein 14 (RNF14) and similar proteins; cd16628"
                     /db_xref="CDD:438290"
     Region          317..369
                     /region_name="BRcat_RBR_RNF14"
                     /note="BRcat domain found in RING finger protein 14
                     (RNF14); cd20341"
                     /db_xref="CDD:439002"
     Region          406..472
                     /region_name="Rcat_RBR_RNF14"
                     /note="Rcat domain found in RING finger protein 14
                     (RNF14); cd20354"
                     /db_xref="CDD:439015"
     CDS             1..512
                     /gene="LOC117611598"
                     /coded_by="XM_034339595.2:204..1742"
                     /db_xref="GeneID:117611598"
ORIGIN      
        1 mdaekqkdev ialesiynte efsyheengs yqctfmifin lppnyyatyk dfrrldepeq
       61 tvkishlppl tlrvvlpeny psklppkftl yspwlhvsll sklckkldel waenkgqeil
      121 ftwvaflqde tleflhiqen lnmnyiytyy ketlekaqnn qknriidnsg kehtedvrnk
      181 mkvgtnskyl skkkliqkry dkraildcpv grnpiqilid ysekrnqief kknfytckic
      241 fvdklgehct qflpcshvfc kdcitnylei rikdgnvqni ycpedectae atpaqikdlv
      301 sselfakyds illsatlatm tdiiycprrn cqypvscepn eqmancpicq yafcvfckmv
      361 yhgiepckly sagthqlvte yqeapddkkl qmeqrygkkq lltlventms enwiktnsqk
      421 cpkckaaiek ldgcnkmkcw rcntpfcwlc ntilnhdrpy ehfedtnskc ynmlyhgmpi
      481 edddededdt vffdllqyld eheseeelfy ef
//
LOCUS       XP_034195487             512 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase RNF14 [Osmia lignaria lignaria].
ACCESSION   XP_034195487
VERSION     XP_034195487.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339596.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195487.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="E3 ubiquitin-protein ligase RNF14"
                     /calculated_mol_wt=60267
     Region          6..132
                     /region_name="RWD_RNF14"
                     /note="RWD domain of RING finger protein 14 (RNF14) and
                     related proteins; cd23820"
                     /db_xref="CDD:467656"
     Region          230..288
                     /region_name="RING-HC_RBR_RNF14"
                     /note="RING finger, HC subclass, found in RING finger
                     protein 14 (RNF14) and similar proteins; cd16628"
                     /db_xref="CDD:438290"
     Region          317..369
                     /region_name="BRcat_RBR_RNF14"
                     /note="BRcat domain found in RING finger protein 14
                     (RNF14); cd20341"
                     /db_xref="CDD:439002"
     Region          406..472
                     /region_name="Rcat_RBR_RNF14"
                     /note="Rcat domain found in RING finger protein 14
                     (RNF14); cd20354"
                     /db_xref="CDD:439015"
     CDS             1..512
                     /gene="LOC117611598"
                     /coded_by="XM_034339596.2:202..1740"
                     /db_xref="GeneID:117611598"
ORIGIN      
        1 mdaekqkdev ialesiynte efsyheengs yqctfmifin lppnyyatyk dfrrldepeq
       61 tvkishlppl tlrvvlpeny psklppkftl yspwlhvsll sklckkldel waenkgqeil
      121 ftwvaflqde tleflhiqen lnmnyiytyy ketlekaqnn qknriidnsg kehtedvrnk
      181 mkvgtnskyl skkkliqkry dkraildcpv grnpiqilid ysekrnqief kknfytckic
      241 fvdklgehct qflpcshvfc kdcitnylei rikdgnvqni ycpedectae atpaqikdlv
      301 sselfakyds illsatlatm tdiiycprrn cqypvscepn eqmancpicq yafcvfckmv
      361 yhgiepckly sagthqlvte yqeapddkkl qmeqrygkkq lltlventms enwiktnsqk
      421 cpkckaaiek ldgcnkmkcw rcntpfcwlc ntilnhdrpy ehfedtnskc ynmlyhgmpi
      481 edddededdt vffdllqyld eheseeelfy ef
//
LOCUS       XP_034195488             450 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin alpha-1 chain [Osmia lignaria lignaria].
ACCESSION   XP_034195488
VERSION     XP_034195488.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339597.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="tubulin alpha-1 chain"
                     /calculated_mol_wt=49894
     Region          1..439
                     /region_name="PTZ00335"
                     /note="tubulin alpha chain; Provisional"
                     /db_xref="CDD:185562"
     CDS             1..450
                     /gene="LOC117611599"
                     /coded_by="XM_034339597.2:157..1509"
                     /db_xref="GeneID:117611599"
ORIGIN      
        1 mrecisvhvg qagvqignac welyclehgi qpdgqmpsdk tigggddsfn tffsetgagk
       61 hvpravfidl eptvvdevrt gtyrqlfhpe qlitgkedaa nnyarghyti gkeivdlvld
      121 rirklsdqct glqgflifhs fgggtgsgft sllmerlsvd ygkksklefa iypapqvsta
      181 vvepynsilt thttlehsdc afmvdneaiy dicrrnldie rptytnlnrl igqivssita
      241 slrfdgalnv dltefqtnlv pyprihfplv tyapvisaek ayheqlsvse itnacfepan
      301 qmvkcdprhg kymaccmlyr gdvvpkdvna aiatiktkrt iqfvdwcptg fkvginyqpp
      361 tvvpggdlak vqravcmlsn ttaiaeawar ldhkfdlmya krafvhwyvg egmeegefse
      421 aredlaalek dyeevgmdsa egeaegveey
//
LOCUS       XP_034195490             440 aa            linear   INV 03-SEP-2025
DEFINITION  glycine receptor subunit alpha alkaliphile isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195490
VERSION     XP_034195490.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339599.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..440
                     /product="glycine receptor subunit alpha alkaliphile
                     isoform X1"
                     /calculated_mol_wt=51064
     Region          1..431
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     CDS             1..440
                     /gene="alka"
                     /coded_by="XM_034339599.2:1479..2801"
                     /db_xref="GeneID:117611600"
ORIGIN      
        1 mkntlltilf ysslkfftev rsiassslvd mknstlksri vlpehyvkei rppakrgtpv
       61 tvdfnifvad insinvedmd frvdmfvrqs wiesrlempe difeegddyv tlppeffdnl
      121 wqpdpyflna kvseiaalnh kfssvtlyrn ktvkysarmh aiiacqmefq lypmdiqtcp
      181 iyiesfsyhk qkvrlrwgag dvtvnpemkl lqydigkprv aeetidymme ksgnfsrlvv
      241 yfrferqigh hliqtfapst lvvmlswfsf wlgldaipgr vallvtsmlt lvtmftglks
      301 dippvayvka ldvwmagcmm fvfaalgefv vvkvldlrnq leydlqtsll srhystriia
      361 mekgqsiwdy dttytpktkn nragskhllq nawidheypi irmyktrsqk idtysrigfp
      421 ilfmlfivly wpilllkkaa
//
LOCUS       XP_076546596             440 aa            linear   INV 03-SEP-2025
DEFINITION  glycine receptor subunit alpha alkaliphile isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546596
VERSION     XP_076546596.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690481.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..440
                     /product="glycine receptor subunit alpha alkaliphile
                     isoform X1"
                     /calculated_mol_wt=51064
     Region          1..431
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     CDS             1..440
                     /gene="alka"
                     /coded_by="XM_076690481.1:1732..3054"
                     /db_xref="GeneID:117611600"
ORIGIN      
        1 mkntlltilf ysslkfftev rsiassslvd mknstlksri vlpehyvkei rppakrgtpv
       61 tvdfnifvad insinvedmd frvdmfvrqs wiesrlempe difeegddyv tlppeffdnl
      121 wqpdpyflna kvseiaalnh kfssvtlyrn ktvkysarmh aiiacqmefq lypmdiqtcp
      181 iyiesfsyhk qkvrlrwgag dvtvnpemkl lqydigkprv aeetidymme ksgnfsrlvv
      241 yfrferqigh hliqtfapst lvvmlswfsf wlgldaipgr vallvtsmlt lvtmftglks
      301 dippvayvka ldvwmagcmm fvfaalgefv vvkvldlrnq leydlqtsll srhystriia
      361 mekgqsiwdy dttytpktkn nragskhllq nawidheypi irmyktrsqk idtysrigfp
      421 ilfmlfivly wpilllkkaa
//
LOCUS       XP_034195491             427 aa            linear   INV 03-SEP-2025
DEFINITION  glycine receptor subunit alpha alkaliphile isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195491
VERSION     XP_034195491.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339600.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="glycine receptor subunit alpha alkaliphile
                     isoform X2"
                     /calculated_mol_wt=49691
     Region          33..418
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     CDS             1..427
                     /gene="alka"
                     /coded_by="XM_034339600.2:673..1956"
                     /db_xref="GeneID:117611600"
ORIGIN      
        1 mkkkkkkkks lysslvdmkn stlksrivlp ehyvkeirpp akrgtpvtvd fnifvadins
       61 invedmdfrv dmfvrqswie srlempedif eegddyvtlp peffdnlwqp dpyflnakvs
      121 eiaalnhkfs svtlyrnktv kysarmhaii acqmefqlyp mdiqtcpiyi esfsyhkqkv
      181 rlrwgagdvt vnpemkllqy digkprvaee tidymmeksg nfsrlvvyfr ferqighhli
      241 qtfapstlvv mlswfsfwlg ldaipgrval lvtsmltlvt mftglksdip pvayvkaldv
      301 wmagcmmfvf aalgefvvvk vldlrnqley dlqtsllsrh ystriiamek gqsiwdydtt
      361 ytpktknnra gskhllqnaw idheypiirm yktrsqkidt ysrigfpilf mlfivlywpi
      421 lllkkaa
//
LOCUS       XP_034195492             408 aa            linear   INV 03-SEP-2025
DEFINITION  glycine receptor subunit alpha alkaliphile isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195492
VERSION     XP_034195492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..408
                     /product="glycine receptor subunit alpha alkaliphile
                     isoform X3"
                     /calculated_mol_wt=47233
     Region          1..>332
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     CDS             1..408
                     /gene="alka"
                     /coded_by="XM_034339601.2:1479..2705"
                     /db_xref="GeneID:117611600"
ORIGIN      
        1 mkntlltilf ysslkfftev rsiassslvd mknstlksri vlpehyvkei rppakrgtpv
       61 tvdfnifvad insinvedmd frvdmfvrqs wiesrlempe difeegddyv tlppeffdnl
      121 wqpdpyflna kvseiaalnh kfssvtlyrn ktvkysarmh aiiacqmefq lypmdiqtcp
      181 iyiesfsyhk qkvrlrwgag dvtvnpemkl lqydigkprv aeetidymme ksgnfsrlvv
      241 yfrferqigh hliqtfapst lvvmlswfsf wlgldaipgr vallvtsmlt lvtmftglks
      301 dippvayvka ldvwmagcmm fvfaalgefv vvkvldlrnq leydlqtsll srhystriia
      361 mekgqsiwdy dttytpktkn nragskhllq nawidheypi ilnihffd
//
LOCUS       XP_034195493             428 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-mannosyl-glycoprotein
            4-beta-N-acetylglucosaminyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034195493
VERSION     XP_034195493.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339602.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..428
                     /product="beta-1,4-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase"
                     /calculated_mol_wt=49770
     Region          144..>285
                     /region_name="Glyco_transf_17"
                     /note="Glycosyltransferase family 17; pfam04724"
                     /db_xref="CDD:428090"
     CDS             1..428
                     /gene="Mgat3"
                     /coded_by="XM_034339602.2:601..1887"
                     /db_xref="GeneID:117611601"
ORIGIN      
        1 mqprldgkla llytllvlqv livmiyvatt ppseltvstt htsvtfvkfe epqkpqiqqt
       61 nykkipiyqt ingevilkdi ktfalfeeyn hticwkygvd dqkmknsenl qkcicthgwh
      121 gsdcgqpevv wraimaskqn iklkhrkiar riihafflhe ynsaiaeviv eelynvvdlf
      181 vicdfsnaed nfhhkllkgl lqhkqkkily invatkihkp srvvskyiwe kmrsvvrnlr
      241 dddiyvttes eqilnsralm flkvyngwpq pigfrlrwsv ygffwqhplk ttitvgacti
      301 glmheayqsn simleqlegd fserdtlglv igdlnhyggw ycylcqapsn iitslqdkik
      361 skeieidkti dvpfiedlig sglwldgktn llrvsksrdl yfapetifnn twkydwlven
      421 fyakldyy
//
LOCUS       XP_034195494             428 aa            linear   INV 03-SEP-2025
DEFINITION  beta-1,4-mannosyl-glycoprotein
            4-beta-N-acetylglucosaminyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034195494
VERSION     XP_034195494.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339603.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..428
                     /product="beta-1,4-mannosyl-glycoprotein
                     4-beta-N-acetylglucosaminyltransferase"
                     /calculated_mol_wt=49770
     Region          144..>285
                     /region_name="Glyco_transf_17"
                     /note="Glycosyltransferase family 17; pfam04724"
                     /db_xref="CDD:428090"
     CDS             1..428
                     /gene="Mgat3"
                     /coded_by="XM_034339603.2:857..2143"
                     /db_xref="GeneID:117611601"
ORIGIN      
        1 mqprldgkla llytllvlqv livmiyvatt ppseltvstt htsvtfvkfe epqkpqiqqt
       61 nykkipiyqt ingevilkdi ktfalfeeyn hticwkygvd dqkmknsenl qkcicthgwh
      121 gsdcgqpevv wraimaskqn iklkhrkiar riihafflhe ynsaiaeviv eelynvvdlf
      181 vicdfsnaed nfhhkllkgl lqhkqkkily invatkihkp srvvskyiwe kmrsvvrnlr
      241 dddiyvttes eqilnsralm flkvyngwpq pigfrlrwsv ygffwqhplk ttitvgacti
      301 glmheayqsn simleqlegd fserdtlglv igdlnhyggw ycylcqapsn iitslqdkik
      361 skeieidkti dvpfiedlig sglwldgktn llrvsksrdl yfapetifnn twkydwlven
      421 fyakldyy
//
LOCUS       XP_034195495             409 aa            linear   INV 03-SEP-2025
DEFINITION  alg7 dolichyl-phosphate N-acetylglucosaminephosphotransferase
            [Osmia lignaria lignaria].
ACCESSION   XP_034195495
VERSION     XP_034195495.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339604.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195495.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="alg7 dolichyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /calculated_mol_wt=46888
     Region          35..321
                     /region_name="GT_GPT_euk"
                     /note="UDP-GlcNAc:dolichol-P GlcNAc-1-P transferase (GPT)
                     catalyzes the transfer of GlcNAc-1-P from UDP-GlcNAc to
                     dolichol-P to form GlcNAc-P-P-dolichol. The reaction is
                     the first step in the assembly of dolichol-linked
                     oligosaccharide intermediates and is...; cd06855"
                     /db_xref="CDD:133465"
     Site            119..120
                     /site_type="other"
                     /note="Mg++ binding site [ion binding]"
                     /db_xref="CDD:133465"
     Site            253..256
                     /site_type="active"
                     /note="putative catalytic motif [active]"
                     /db_xref="CDD:133465"
     CDS             1..409
                     /gene="Alg7"
                     /coded_by="XM_034339604.2:1..1230"
                     /db_xref="GeneID:117611602"
ORIGIN      
        1 mfkmkydndt wkfifpilin flmsvsvyfl tvrlipkikd mfikanlygi dlnknsgekv
       61 pealgvvtgc lflitlflfi pipftnyifn dtnfphsefm eflaallsic cmlllgfadd
      121 vldlrwrhkl llptiaslpl lmvyyinfns tliivpkplr swfgfsvdlw ifyylymgml
      181 avfctnaini laginglevg qslvialsil vfniielsgn lwkahqfsly fmfpyiatsl
      241 gllkfnwypa qvfvgdtfcy lsgmtfavvg iighfskttl lffipqilnf lysvpqlfhf
      301 ipcprhrlpk ynketdklti sntvfnkkdi gvigsfilwi fgklniikwe eddkgiitcn
      361 nftlinfvli kigpmreptl tltllliqvv ssllafviry plasifydv
//
LOCUS       XP_034195496             350 aa            linear   INV 03-SEP-2025
DEFINITION  carbohydrate sulfotransferase 11 [Osmia lignaria lignaria].
ACCESSION   XP_034195496
VERSION     XP_034195496.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339605.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195496.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="carbohydrate sulfotransferase 11"
                     /calculated_mol_wt=40915
     Region          100..339
                     /region_name="Sulfotransfer_2"
                     /note="Sulfotransferase family; pfam03567"
                     /db_xref="CDD:427369"
     CDS             1..350
                     /gene="LOC117611603"
                     /coded_by="XM_034339605.2:175..1227"
                     /db_xref="GeneID:117611603"
ORIGIN      
        1 mirsykrgti qilkciiscg laffviklll igdeskplpe fgdreklmie aendnsnris
       61 tirntcqkyn lgiykkiggs sifkhpptpq ysvfyivrnh glsycpiyka gsttwiynlc
      121 llmnvsekel nggkeqisti arrlipelef peadqalkat kkllvvrqpf erllsayrdk
      181 lensvagreh gtlhfyrkyg gkivekyrek nftkprddqv irrkdipepa gieptfkefv
      241 qylidtdlas ygddhwmpyy ffctpcivny diiakvetlw rdqiytihkl qlqevikprw
      301 rhsggysnas riyfsqlnrd mveklyekfk ldfelfdysp kvyfqyatst
//
LOCUS       XP_034195498             339 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-galactose transporter senju [Osmia lignaria lignaria].
ACCESSION   XP_034195498
VERSION     XP_034195498.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339607.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="UDP-galactose transporter senju"
                     /calculated_mol_wt=38375
     Region          49..320
                     /region_name="Nuc_sug_transp"
                     /note="Nucleotide-sugar transporter; pfam04142"
                     /db_xref="CDD:398009"
     CDS             1..339
                     /gene="senju"
                     /coded_by="XM_034339607.2:372..1391"
                     /db_xref="GeneID:117611604"
ORIGIN      
        1 mgsinwgelf pgrwspvifi symalfvnqg iivtwsqrdg hyeynivmvv lmtevlklla
       61 siilyckdns vssliqevtr nkkvlllyti psflyclynn lafinlaafd pttyyvllql
      121 rvvmtgiifq vvfdkklslk qwfslvllti gcmvkhleld lnvnifkakv nlnsniilif
      181 vqticsclag vyneyllkeq gasinifvqn vfmyvdsifc nfsvfvllyi fenstsnifk
      241 daspsillqp kvilimlnnt aigiitslfl knlnsilktf asalellfta vlcwlifsip
      301 ihlntvvsia mvsyavilys qhpvqnvktk ertkadsiv
//
LOCUS       XP_034195499             337 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195499
VERSION     XP_034195499.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339608.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="very long chain fatty acid elongase 4 isoform
                     X1"
                     /calculated_mol_wt=38981
     Region          55..291
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..337
                     /gene="LOC117611605"
                     /coded_by="XM_034339608.2:303..1316"
                     /db_xref="GeneID:117611605"
ORIGIN      
        1 mkankwiyqr rctctcykft amaniinsta tlisdvydyy lwtlslader trgwllvdsp
       61 kptfiytmly llivwagpki mknrkafklt walvpynlam allnayiaiq lfvastrlry
      121 syvcqpirhv trpdelqian avwwyyfskl lefcdtfffi lrkkdnqlsf lhvyhhstmf
      181 slwwigikwv psgstflpam vnsfihvlmy syyglaalgp svtkylwwkk yltilqliqf
      241 ttalilging irsgcdfplw mqyalviymv sfivlfgnfy akayiakgkk ayaerqlekm
      301 kvaaqlktel tdgavrngnv tnghangyan gvskktq
//
LOCUS       XP_034195500             316 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195500
VERSION     XP_034195500.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339609.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="very long chain fatty acid elongase 4 isoform
                     X2"
                     /calculated_mol_wt=36383
     Region          34..270
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..316
                     /gene="LOC117611605"
                     /coded_by="XM_034339609.2:272..1222"
                     /db_xref="GeneID:117611605"
ORIGIN      
        1 maniinstat lisdvydyyl wtlsladert rgwllvdspk ptfiytmlyl livwagpkim
       61 knrkafkltw alvpynlama llnayiaiql fvastrlrys yvcqpirhvt rpdelqiana
      121 vwwyyfskll efcdtfffil rkkdnqlsfl hvyhhstmfs lwwigikwvp sgstflpamv
      181 nsfihvlmys yyglaalgps vtkylwwkky ltilqliqft talilgingi rsgcdfplwm
      241 qyalviymvs fivlfgnfya kayiakgkka yaerqlekmk vaaqlktelt dgavrngnvt
      301 nghangyang vskktq
//
LOCUS       XP_034195501             316 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195501
VERSION     XP_034195501.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339610.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="very long chain fatty acid elongase 4 isoform
                     X2"
                     /calculated_mol_wt=36383
     Region          34..270
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..316
                     /gene="LOC117611605"
                     /coded_by="XM_034339610.2:146..1096"
                     /db_xref="GeneID:117611605"
ORIGIN      
        1 maniinstat lisdvydyyl wtlsladert rgwllvdspk ptfiytmlyl livwagpkim
       61 knrkafkltw alvpynlama llnayiaiql fvastrlrys yvcqpirhvt rpdelqiana
      121 vwwyyfskll efcdtfffil rkkdnqlsfl hvyhhstmfs lwwigikwvp sgstflpamv
      181 nsfihvlmys yyglaalgps vtkylwwkky ltilqliqft talilgingi rsgcdfplwm
      241 qyalviymvs fivlfgnfya kayiakgkka yaerqlekmk vaaqlktelt dgavrngnvt
      301 nghangyang vskktq
//
LOCUS       XP_034195502             316 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195502
VERSION     XP_034195502.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="very long chain fatty acid elongase 4 isoform
                     X2"
                     /calculated_mol_wt=36383
     Region          34..270
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..316
                     /gene="LOC117611605"
                     /coded_by="XM_034339611.2:146..1096"
                     /db_xref="GeneID:117611605"
ORIGIN      
        1 maniinstat lisdvydyyl wtlsladert rgwllvdspk ptfiytmlyl livwagpkim
       61 knrkafkltw alvpynlama llnayiaiql fvastrlrys yvcqpirhvt rpdelqiana
      121 vwwyyfskll efcdtfffil rkkdnqlsfl hvyhhstmfs lwwigikwvp sgstflpamv
      181 nsfihvlmys yyglaalgps vtkylwwkky ltilqliqft talilgingi rsgcdfplwm
      241 qyalviymvs fivlfgnfya kayiakgkka yaerqlekmk vaaqlktelt dgavrngnvt
      301 nghangyang vskktq
//
LOCUS       XP_034195503             161 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar H+ ATP synthase 16 kDa proteolipid subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034195503
VERSION     XP_034195503.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339612.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="vacuolar H+ ATP synthase 16 kDa proteolipid
                     subunit"
                     /calculated_mol_wt=16407
     Region          17..124
                     /region_name="V_ATP_synt_C"
                     /note="vacuolar ATP synthase 16 kDa proteolipid subunit;
                     TIGR01100"
                     /db_xref="CDD:130170"
     Region          92..159
                     /region_name="ATP-synt_Vo_c_ATP6C_rpt2"
                     /note="V-type proton ATPase 16 kDa proteolipid subunit
                     (ATP6C/ATP6V0C/ATP6L/ATPL) and similar proteins; cd18176"
                     /db_xref="CDD:349416"
     CDS             1..161
                     /gene="Vha16"
                     /coded_by="XM_034339612.2:180..665"
                     /db_xref="GeneID:117611606"
ORIGIN      
        1 msssgeltee rpvyapffgv mgaasaiifs algaaygtak sgtgiaamsv mrpelimksi
       61 ipvvmagiia iyglvvavli agdleepsky slfkgfihlg aglavgfsgl aagfaigivg
      121 dagvrgtaqq prlfvgmili lifaevlgly glivaiylyt k
//
LOCUS       XP_034196364             425 aa            linear   INV 03-SEP-2025
DEFINITION  putative fatty acyl-CoA reductase CG5065 [Osmia lignaria lignaria].
ACCESSION   XP_034196364
VERSION     XP_034196364.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340473.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196364.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="putative fatty acyl-CoA reductase CG5065"
                     /calculated_mol_wt=47927
     Region          23..244
                     /region_name="NADB_Rossmann"
                     /note="Rossmann-fold NAD(P)(+)-binding proteins; cl21454"
                     /db_xref="CDD:473865"
     Site            order(29,31..32,34,55..57,121..123,144..146,173..176)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Region          290..379
                     /region_name="Sterile"
                     /note="Male sterility protein; pfam03015"
                     /db_xref="CDD:460779"
     CDS             1..425
                     /gene="LOC117611948"
                     /coded_by="XM_034340473.2:640..1917"
                     /db_xref="GeneID:117611948"
ORIGIN      
        1 mellsfpigq sksmalsewy adrellltgv tsdfgralle kilrsfpnvk vytvlrsrng
       61 ldkedrirni flsprfervr qelpdaisrv kafegnlvyd glgtmkedrk llqnvsvvlh
      121 aggpcqklfe fcqklprlev aaivssvfqq egrtsegsre edvadvpvaf lrlpligpay
      181 repmpgfvdv ltgptalivg agfalgksql raeiipidla vnslivsawe ratiknvqet
      241 vvynaipvdc twaelikkgr rgnrkftypt fglrgmtsia glhwilvllf ewlpsafcdt
      301 ilsqlgakkr lleeqrrvrn alrslesiss rswsaernrl yllhqrlsse eqdafpvaae
      361 idvesyvlca taaarkycvd dsnigivkif rllfflsiav aflsmfffnk yrtsetvtrr
      421 eieqf
//
LOCUS       XP_034195510             762 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein 28 [Osmia lignaria lignaria].
ACCESSION   XP_034195510
VERSION     XP_034195510.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339619.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195510.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..762
                     /product="RNA-binding protein 28"
                     /calculated_mol_wt=87434
     Region          44..119
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          194..269
                     /region_name="RRM2_RBM28_like"
                     /note="RNA recognition motif 2 (RRM2) found in RNA-binding
                     protein 28 (RBM28) and similar proteins; cd12414"
                     /db_xref="CDD:409848"
     Region          372..455
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          519..611
                     /region_name="RRM4_RBM28_like"
                     /note="RNA recognition motif 4 (RRM4) found in RNA-binding
                     protein 28 (RBM28) and similar proteins; cd12416"
                     /db_xref="CDD:409850"
     CDS             1..762
                     /gene="LOC117611609"
                     /coded_by="XM_034339619.2:343..2631"
                     /db_xref="GeneID:117611609"
ORIGIN      
        1 mrntgkrgdk kklswiyrkk irfqkskrgt eitnnngeed kipriivrnv pfkatiedvq
       61 klyepfgqis dinfpkradg ipvgccfiqf kqleqaskai fntnkkeflg riissswaip
      121 kskyseklkn esivnldngk vddnnitkdn alnndnenqd fdksddfdkk ekltqirker
      181 rkllkeknrk krariivrnl sfqvteenlk dhfsqygtie evqilkkedg khvgcaflqf
      241 nhvqsaakai hyanlqplld rpivvdwavp knkfcknsen etngavevki ekdsdaedtd
      301 lavttgdglc sedeitdahd sdsddlveae vkseceneal dqelsevdek dkvnekeeik
      361 rpryeshdvs egktvflknv pfsikneelk kymeqfgpiy yalvcidplt eyskgtafvk
      421 fknvedaekc lsagtelqme dqiieaqral dkneienkan skhqrqkdsr nlylvkegvv
      481 lagspssvgv saadmakrlq ieqwksqilr nlnmfvsrvr lvihnlpatl nddklrkive
      541 qhsppnatik evrimrdlkn vdmkgvgksk eygfvaftrh edaletlrni nnnpniftpk
      601 rrpivsfsie nrvlvnakqk rlqksrennp lcsennnnkr kgekadaevt ikklkvgnvd
      661 ksdkksyvgm igkpgenklr skfniknqai lhnqtlkkek kmkksskkld ikrranianr
      721 neikpkqgtk vhaddvnldk lvhkykntlk sielrkskwy es
//
LOCUS       XP_034195504             483 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein KIAA0513 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195504
VERSION     XP_034195504.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339613.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="uncharacterized protein KIAA0513 isoform X1"
                     /calculated_mol_wt=54220
     Region          <298..>389
                     /region_name="SBF2"
                     /note="Myotubularin protein; pfam12335"
                     /db_xref="CDD:463536"
     CDS             1..483
                     /gene="LOC117611607"
                     /coded_by="XM_034339613.2:235..1686"
                     /db_xref="GeneID:117611607"
ORIGIN      
        1 mvdvttapps tggatligrt rgalrsvrsa lggsgeylqg lgsrigsals ssleeseltt
       61 tpstppsspq alpqtdkpee qlarrklrlp rfgagmcyed yeaiarhkle rqgsasmsrc
      121 mrkyppgmty eeitssrtvg kktqedkied dnspdrdsqe sieplqekdi katgpdpfdk
      181 lnyraarapt ryqtvvfrld mqpcssdsms dqdgygpsts ldsnmdgrrl wqrsgssgsg
      241 qswasslsad smseesaadf mktfvpllfd apnsidqeqk asfgqmvlte sgriwfsrvv
      301 narrarpcvt eesfyslaqh favalfeche addfapaksl mnmcftfyhe vevpglepyr
      361 eylythlrvq piwtsmrfwt aaffdavqce rasrpvpprp ksldqeaiai edrkfqaniv
      421 fgqlgtftcn mhafglsrtl cleflrkqcv ianltkeqek mlrdniermf detepwrree
      481 rvs
//
LOCUS       XP_034195505             483 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein KIAA0513 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195505
VERSION     XP_034195505.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339614.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="uncharacterized protein KIAA0513 isoform X1"
                     /calculated_mol_wt=54220
     Region          <298..>389
                     /region_name="SBF2"
                     /note="Myotubularin protein; pfam12335"
                     /db_xref="CDD:463536"
     CDS             1..483
                     /gene="LOC117611607"
                     /coded_by="XM_034339614.2:136..1587"
                     /db_xref="GeneID:117611607"
ORIGIN      
        1 mvdvttapps tggatligrt rgalrsvrsa lggsgeylqg lgsrigsals ssleeseltt
       61 tpstppsspq alpqtdkpee qlarrklrlp rfgagmcyed yeaiarhkle rqgsasmsrc
      121 mrkyppgmty eeitssrtvg kktqedkied dnspdrdsqe sieplqekdi katgpdpfdk
      181 lnyraarapt ryqtvvfrld mqpcssdsms dqdgygpsts ldsnmdgrrl wqrsgssgsg
      241 qswasslsad smseesaadf mktfvpllfd apnsidqeqk asfgqmvlte sgriwfsrvv
      301 narrarpcvt eesfyslaqh favalfeche addfapaksl mnmcftfyhe vevpglepyr
      361 eylythlrvq piwtsmrfwt aaffdavqce rasrpvpprp ksldqeaiai edrkfqaniv
      421 fgqlgtftcn mhafglsrtl cleflrkqcv ianltkeqek mlrdniermf detepwrree
      481 rvs
//
LOCUS       XP_034195506             483 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein KIAA0513 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195506
VERSION     XP_034195506.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339615.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="uncharacterized protein KIAA0513 isoform X1"
                     /calculated_mol_wt=54220
     Region          <298..>389
                     /region_name="SBF2"
                     /note="Myotubularin protein; pfam12335"
                     /db_xref="CDD:463536"
     CDS             1..483
                     /gene="LOC117611607"
                     /coded_by="XM_034339615.2:127..1578"
                     /db_xref="GeneID:117611607"
ORIGIN      
        1 mvdvttapps tggatligrt rgalrsvrsa lggsgeylqg lgsrigsals ssleeseltt
       61 tpstppsspq alpqtdkpee qlarrklrlp rfgagmcyed yeaiarhkle rqgsasmsrc
      121 mrkyppgmty eeitssrtvg kktqedkied dnspdrdsqe sieplqekdi katgpdpfdk
      181 lnyraarapt ryqtvvfrld mqpcssdsms dqdgygpsts ldsnmdgrrl wqrsgssgsg
      241 qswasslsad smseesaadf mktfvpllfd apnsidqeqk asfgqmvlte sgriwfsrvv
      301 narrarpcvt eesfyslaqh favalfeche addfapaksl mnmcftfyhe vevpglepyr
      361 eylythlrvq piwtsmrfwt aaffdavqce rasrpvpprp ksldqeaiai edrkfqaniv
      421 fgqlgtftcn mhafglsrtl cleflrkqcv ianltkeqek mlrdniermf detepwrree
      481 rvs
//
LOCUS       XP_034195507             477 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein KIAA0513 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195507
VERSION     XP_034195507.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339616.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="uncharacterized protein KIAA0513 isoform X2"
                     /calculated_mol_wt=53463
     Region          <298..>389
                     /region_name="SBF2"
                     /note="Myotubularin protein; pfam12335"
                     /db_xref="CDD:463536"
     CDS             1..477
                     /gene="LOC117611607"
                     /coded_by="XM_034339616.2:235..1668"
                     /db_xref="GeneID:117611607"
ORIGIN      
        1 mvdvttapps tggatligrt rgalrsvrsa lggsgeylqg lgsrigsals ssleeseltt
       61 tpstppsspq alpqtdkpee qlarrklrlp rfgagmcyed yeaiarhkle rqgsasmsrc
      121 mrkyppgmty eeitssrtvg kktqedkied dnspdrdsqe sieplqekdi katgpdpfdk
      181 lnyraarapt ryqtvvfrld mqpcssdsms dqdgygpsts ldsnmdgrrl wqrsgssgsg
      241 qswasslsad smseesaadf mktfvpllfd apnsidqeqk asfgqmvlte sgriwfsrvv
      301 narrarpcvt eesfyslaqh favalfeche addfapaksl mnmcftfyhe vevpglepyr
      361 eylythlrvq piwtsmrfwt aaffdavqce rasrpvpprp ksldqeaiai edrkfqaniv
      421 fgqlgtftcn mhafglsrtl cleflrkqcv ianltkeqek mlrdniermf detepwr
//
LOCUS       XP_034195508             469 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein KIAA0513 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195508
VERSION     XP_034195508.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339617.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="uncharacterized protein KIAA0513 isoform X3"
                     /calculated_mol_wt=52560
     Region          <284..>375
                     /region_name="SBF2"
                     /note="Myotubularin protein; pfam12335"
                     /db_xref="CDD:463536"
     CDS             1..469
                     /gene="LOC117611607"
                     /coded_by="XM_034339617.2:235..1644"
                     /db_xref="GeneID:117611607"
ORIGIN      
        1 mvdvttapps tggatligrt rgalrsvrsa lggsgeylqg lgsrigsals ssleeseltt
       61 tpstppsspq alpqtdkpee qlarrklrlp rfgagmcyed yeaiarhkle rqgsasmsrc
      121 mrkyppgmty eeitssrtvg kktqedkied dnspdrdsqe sieplqekdi katgpdpfdk
      181 lnyradmqpc ssdsmsdqdg ygpstsldsn mdgrrlwqrs gssgsgqswa sslsadsmse
      241 esaadfmktf vpllfdapns idqeqkasfg qmvltesgri wfsrvvnarr arpcvteesf
      301 yslaqhfava lfecheaddf apakslmnmc ftfyhevevp glepyreyly thlrvqpiwt
      361 smrfwtaaff davqcerasr pvpprpksld qeaiaiedrk fqanivfgql gtftcnmhaf
      421 glsrtlclef lrkqcvianl tkeqekmlrd niermfdete pwrreervs
//
LOCUS       XP_076546597             431 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610677 [Osmia lignaria lignaria].
ACCESSION   XP_076546597
VERSION     XP_076546597.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690482.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..431
                     /product="uncharacterized protein LOC117610677"
                     /calculated_mol_wt=48548
     CDS             1..431
                     /gene="LOC117610677"
                     /coded_by="XM_076690482.1:1651..2946"
                     /db_xref="GeneID:117610677"
ORIGIN      
        1 msvtprgssr rrrsetedpf srklrcleee yrrsrsrhgr trsrrrssds shnrrssadd
       61 fdkengeria giyrrtshsn qnhltsskrd rssslfdevs gechrraasr ssrrhsysse
      121 kdtqisaies dreggernpr dtsrpshisl esakqtdaeg ivsameldev tlgllgddpa
      181 kdtqkgpdih vavaeiwdki lrdglskdqk rdlqekypti gncpltrapk lnpeiktals
      241 alagkkdhyq ylaqnqlgag inaigaalte vlkaeksiel avdstkfier ladagrilad
      301 lhhdmsktrk sfivpglnpi vkqiadespi dgllfgekfs enlkaakvme kaskdvikqg
      361 gsgyrksigi tsrdenrryq ssrwkqsgyp shhqgeklnw rrsfgmsyrg tqrkgqkske
      421 prpkdnrqhk d
//
LOCUS       XP_034195420             715 aa            linear   INV 03-SEP-2025
DEFINITION  XPG-like endonuclease [Osmia lignaria lignaria].
ACCESSION   XP_034195420
VERSION     XP_034195420.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339529.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195420.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..715
                     /product="XPG-like endonuclease"
                     /calculated_mol_wt=81790
     Region          2..216
                     /region_name="PIN_GEN1"
                     /note="FEN-like PIN domains of Gap Endonuclease 1, a
                     structure-specific, divalent-metal-ion dependent, 5'
                     nuclease and homologs; cd09869"
                     /db_xref="CDD:350217"
     Site            order(16,30,37,53..54,57..58,74,133,135,154,156,169..170)
                     /site_type="active"
                     /db_xref="CDD:350217"
     Site            order(80..91,103..110)
                     /site_type="other"
                     /note="helical arch"
                     /db_xref="CDD:350217"
     Region          200..329
                     /region_name="H3TH_StructSpec-5'-nucleases"
                     /note="H3TH domains of structure-specific 5' nucleases (or
                     flap endonuclease-1-like) involved in DNA replication,
                     repair, and recombination; cl22433"
                     /db_xref="CDD:473957"
     Site            order(210..211,213,220..222,227)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:188616"
     Site            213
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:188616"
     Region          396..469
                     /region_name="Chromo_2"
                     /note="Chromatin organization modifier domain 2;
                     pfam18704"
                     /db_xref="CDD:465841"
     CDS             1..715
                     /gene="Gen"
                     /coded_by="XM_034339529.2:99..2246"
                     /db_xref="GeneID:117611564"
ORIGIN      
        1 mgvkdlwnil splcerkpmf elqgktiaid mscwvvdsqt vtdnhvqpkm ylrnlyfrta
       61 fllmqdilpv fvlegkapdl klntiakrnd nrngvrerkt srkegrpqfn rilkeckell
      121 nymglkcvqg hgeaeamcay lnedglvhgc isqdsdcfly gakvvyrnfc asargnrggt
      181 ggavdeynid kiekildlgr nkmialallc gcdydeglng vgkeaamklf kivkdqdiie
      241 rikswktddi ldhkesells snmcsscghs gkvqkhtksg cidcgtvvkc nnsykeqral
      301 ilneislrkk allvpdfpkq elideflvrk dsvptkldiq wkqpqvdkfi dfverhlcwe
      361 pqyafekifp latrwqllyl pnisiehrls ipdlfipeki kkirnirsva cyeiiwktdh
      421 avieklkeyi alskdndasn teilseltsi epqaaakecy telveifent rnvktkkrtt
      481 knkaqnnvds ggiiknkatk rrqkktekap isvennrkid efigkdrcls leesfgrmsi
      541 tpkrskpldk ekqeenirik rgpqlnkvlq iekvnsklnn tldrmfnels pddfvsdndd
      601 hdlnmseiid nickervfqf dvmegadvck nersenfnad sstkcyeteh tecnavades
      661 inefpsises yvplheriga fnesnefrni htkkndftlg indllndtda dqael
//
LOCUS       XP_034195421             192 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611565 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195421
VERSION     XP_034195421.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339530.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195421.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..192
                     /product="uncharacterized protein LOC117611565 isoform X1"
                     /calculated_mol_wt=20362
     Region          24..74
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..192
                     /gene="LOC117611565"
                     /coded_by="XM_034339530.2:282..860"
                     /db_xref="GeneID:117611565"
ORIGIN      
        1 mkatfavafa avfvtfaygt rrvhcppend vdvtllpnpd dcttyyscnq giawpmycpg
       61 rlhfnaelrv cdspehakca spslniaaek hrptppgtrp tppgtrptpp gtrptppgtr
      121 pprpgtrppr pgtrpprpgt rpphttvpst tvppttvppt tvppttvppt tvppttelpt
      181 tawptavppt tv
//
LOCUS       XP_076546598             168 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611565 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546598
VERSION     XP_076546598.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690483.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..168
                     /product="uncharacterized protein LOC117611565 isoform X2"
                     /calculated_mol_wt=17817
     Region          24..74
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..168
                     /gene="LOC117611565"
                     /coded_by="XM_076690483.1:279..785"
                     /db_xref="GeneID:117611565"
ORIGIN      
        1 mkatfavafa avfvtfaygt rrvhcppend vdvtllpnpd dcttyyscnq giawpmycpg
       61 rlhfnaelrv cdspehakca spslniaaek hrptppgtrp tppgtrptpp gtrptppgtr
      121 pprpgtrppp ttvppttvpp ttvppttvpp ttelpttawp tavppttv
//
LOCUS       XP_034196203             316 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611886 [Osmia lignaria lignaria].
ACCESSION   XP_034196203
VERSION     XP_034196203.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340312.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196203.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="uncharacterized protein LOC117611886"
                     /calculated_mol_wt=35137
     CDS             1..316
                     /gene="LOC117611886"
                     /coded_by="XM_034340312.2:71..1021"
                     /db_xref="GeneID:117611886"
ORIGIN      
        1 mmarncdscs fvlrcvfltc lvilhaakge scdktkcpgp lryykelgct pvyknpddcc
       61 aeeydcshlk slskdkcyan gheynigdkl rdedanpcdv ecrcrsyedr aasfvcaald
      121 cgiaftpgcy nknsvgqccp gplvcpekge eratcevdgk iymdgeffkp nssdpdlscy
      181 cqpgykgenv epfckmpnrs ycsplfrnap aihqncapvf ynnqnpqqdc syasrcqnan
      241 dtvihkhesg kslseednkt cqfgdmkmhi gdelnqgtny dstcvkcvce vppyptcqrl
      301 pdnecdvtdh glfinl
//
LOCUS       XP_076546599             201 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305730 [Osmia lignaria lignaria].
ACCESSION   XP_076546599
VERSION     XP_076546599.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690484.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="uncharacterized protein LOC143305730"
                     /calculated_mol_wt=23405
     Region          <3..137
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     Region          137..196
                     /region_name="Transp_Tc5_C"
                     /note="Tc5 transposase C-terminal domain; pfam04236"
                     /db_xref="CDD:398081"
     CDS             1..201
                     /gene="LOC143305730"
                     /coded_by="XM_076690484.1:10..615"
                     /db_xref="GeneID:143305730"
ORIGIN      
        1 mfkptnilvt astsgkltne hfkiwlkevy fpntndktal lldswsghcg rsiveatpen
       61 kelvvftipa gttkylqpld vygfrvwknf vrrfsdtvil lhyeinlhtr dnilklqsld
      121 hnqfssprfe nfikyswyks gyvssrpphf enpvefcyaa dneprchvcg dvaiitcawc
      181 rkplclkhff ddyhfcheyv p
//
LOCUS       XP_076546601             284 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610935 [Osmia lignaria lignaria].
ACCESSION   XP_076546601
VERSION     XP_076546601.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690486.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="uncharacterized protein LOC117610935"
                     /calculated_mol_wt=32478
     CDS             1..284
                     /gene="LOC117610935"
                     /coded_by="XM_076690486.1:1..855"
                     /db_xref="GeneID:117610935"
ORIGIN      
        1 mvyinpinvl tllsiafnsv dkkvtpvnsa elelkdilyd iivqrmedpt fnieqetyln
       61 ieeefanged pdvnfqwlke temdltetdl tdeeeqdqql ekctlseapt syeyrinave
      121 ywkngktkrl kletvqsrfr tvksvrqlrk waaqvemggt rldkllriad ytyknviqai
      181 rsniivhdid irrwalqakl eedlasftsf enlynsdqsg fnlelhsgrt lalkgtkkve
      241 aviqsipstt hsytiqptis asgkllsplf vvlketsgef gprv
//
LOCUS       XP_076546602             336 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305731 [Osmia lignaria lignaria].
ACCESSION   XP_076546602
VERSION     XP_076546602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690487.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="uncharacterized protein LOC143305731"
                     /calculated_mol_wt=39378
     CDS             1..336
                     /gene="LOC143305731"
                     /coded_by="XM_076690487.1:353..1363"
                     /db_xref="GeneID:143305731"
ORIGIN      
        1 mrfmdngiat ilvlcekrtv tlyiaifnli lklpnninfi msdyeiaavk avckffpnah
       61 ihgcwfhycq tvlrkwrkfg ltnapnivvr mtmslpllpe mkfqegllii qreadiisnn
      121 fpnvllfmay mrtnwlkmas rvsvhncpvr tnniaksfhn iaalklgkqn invwtfleki
      181 rdllidqeld fnrlqngiac rrprtygnin crkkiikaqa eydterltle eflrifnyed
      241 tifqsnkidt vaiillkrli vqwkkngcig eeveepylir lryrriprkp ktgkhqnaek
      301 rnyrflhilq vksrtklets ifsaqtqmlv niqaip
//
LOCUS       XP_076546603            2274 aa            linear   INV 03-SEP-2025
DEFINITION  sushi, von Willebrand factor type A, EGF and pentraxin
            domain-containing protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_076546603
VERSION     XP_076546603.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690488.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2274
                     /product="sushi, von Willebrand factor type A, EGF and
                     pentraxin domain-containing protein 1"
                     /calculated_mol_wt=256069
     Region          100..275
                     /region_name="VWA"
                     /note="von Willebrand factor type A domain; pfam00092"
                     /db_xref="CDD:459670"
     Region          334..386
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          404..460
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(414,434)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          465..532
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(475,505)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          536..591
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Site            order(546,569)
                     /site_type="active"
                     /note="receptor-ligand interactions [active]"
                     /db_xref="CDD:153056"
     Region          600..681
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          683..764
                     /region_name="HYR"
                     /note="HYR domain; pfam02494"
                     /db_xref="CDD:460572"
     Region          1053..1100
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          1121..1162
                     /region_name="Ephrin_rec_like"
                     /note="Tyrosine-protein kinase ephrin type A/B
                     receptor-like; pfam07699"
                     /db_xref="CDD:429604"
     Region          1173..1205
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1209..1248
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1251..1286
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1293..1323
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1369..1404
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1404..1602
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     Site            order(1463,1539,1541,1548,1551)
                     /site_type="active"
                     /note="calcium mediated ligand binding site [active]"
                     /db_xref="CDD:238086"
     Region          1611..1884
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          <1940..1980
                     /region_name="CCP"
                     /note="Complement control protein (CCP) modules (aka short
                     consensus repeats SCRs or SUSHI repeats) have been
                     identified in several proteins of the complement system;
                     cd00033"
                     /db_xref="CDD:153056"
     Region          <2060..2219
                     /region_name="PHA02927"
                     /note="secreted complement-binding protein; Provisional"
                     /db_xref="CDD:222943"
     Region          2160..2217
                     /region_name="Sushi"
                     /note="Sushi repeat (SCR repeat); pfam00084"
                     /db_xref="CDD:459664"
     CDS             1..2274
                     /gene="LOC117610767"
                     /coded_by="XM_076690488.1:265..7089"
                     /db_xref="GeneID:117610767"
ORIGIN      
        1 migsitrkci ligllsrlli aiaffndfpr fnedfhfevg avnntaslii nfkekrhvnq
       61 hefneslenv qrtfqnkana lshllkihid hlrnetdqie lvflvdasgs vglknfqsel
      121 nfvknllsdf svepsvtrva ivtfggrrni rrnvdqisrs sgidnkcyll nkqlnnityt
      181 gggtytkgal leafrilekg rpnarkavfl itdgfsnggd prpasnllkn agatiftfgi
      241 rtgnvdelhd iasypgytys yfldsftefe alarralhrd lktgkyvpvt ypdncnllcr
      301 nisevgnern ccdnsatcac gtatghysci cptgyfgsgf kgscqpcpng tyasgeisgd
      361 stsmcipcpd anhvtikipa tsvehcvcds gfttdghkce vitcpklkip engylvkasa
      421 cnnvvhaacg ircrigfyli gdsiqlcgkd gswsgnqpkc llktcpvlrv pahgrmrcqt
      481 eedqhritdn staypidtrc qfkceigyql rgskirnclp laqwdglkat ckaikceplk
      541 qiangqispk acsgpekvpf gtnctitckk gfvlegpksr tcggrsgiwt qrrtvnrcad
      601 ktppsitcps diitenlpgk nyayvnwtap taidnvdesp tlwskpyinf pwkvkigtrt
      661 vvyvsqdssg nkarckfkvk vldkeppmie ncinppilyt dngigvynvt wdepgfydns
      721 kspvqveqny hpgenifpig stkvvytaid mydnnascil nvtikdicde vpkvlnsysr
      781 csnlsnetne cvincdegfg fatedsnhqi vedilllkcn tnnsswteed ympdcsessv
      841 pktvsqegsi vlegnvsvvc dnqttlrels qyitadlrst lldicgndie cnlitfdpec
      901 eenlsaggnv ysnltrrrre lnyydisste lheidtmimn gvnnkekrna klkdnnknka
      961 kmkkkkekie ikfkflgkii eenfenpreg vqklrqkies lsqsgklnll nnktneeiar
     1021 lalnlhlvfk nfqelcepgt vlkkhtcvkc plgtffdsst krcqpcpfge yqdttgslkc
     1081 krcpehtftr rirskslrdc irvcrpgyys rrrryydtgf alepclmcdi gfyqseygqt
     1141 hclpcppntt tenrgtkyfn dclpfyetes gvcetescln ggqctqeeds fsceclnyyi
     1201 gskcetfrnp cdsspclneg tcnvqnfgng ticyncdctn gysgsncely idecvvnpcl
     1261 nngtcvsten dytceckhgf egefceiamn hcdpspcmeg stckninetw qcfckpgflg
     1321 rycnllpcdw lpcnensyci nieernatrm syrcecidgy igencttrid hckndpclnn
     1381 gkcvngvfny tcvcsvpftg rhcetelssd yvvqftksgt tdyvsmkgpv tnlleltmcl
     1441 wlqskdtfny gtvlsyatsy ydnaftltdy nglviyingq kvvtdikvnd ghwhficitw
     1501 eseygywnif idgflrdngt hlakgsvvqg sgtlvigqeq drmgggfsel esflgkltll
     1561 diwstvlktt dvhhlfntcd kyhgnviaws qvqehihgdv ailgspfcrg cplpvvpfkg
     1621 ninvsddale ityycdngym vrfygkeyrs lrvkclkqgq wegyytpvct rrkcgfpgyf
     1681 prgrmhgrsy lygdeirysc ltgyelrgnp rrictadgrw sglppicigk tcknllapen
     1741 gdieyvieey erddlsilqv gqqiefkcdd gfhligekfl tclesgvwdh drpicvangc
     1801 pppkeiqhgy mitensnnlq epstrnfilq ntnntferny hfdeiieync qsgykfrgny
     1861 nlstefklrc aengtwigfv pdcvplkcpw psiinnarif lktendtiel fkrrptnvtn
     1921 leiiseesed sfeedtilee qfingseili kcdmgyklid sdvricmdna ewssgsssce
     1981 pqecpllthp vfglfykgmn ldnhtdilrn dqnmkwtdrd yysgsygnie ffvegdtymk
     2041 riilscrnni eiklndesvs nltwscneyg ewtiinsmln tttienllkn klndicqelm
     2101 casvtipeyg yivnnnysri instvtfkcr qgyiiegnem svclpnitws aipsckpvtc
     2161 gkppkltnav ikdtvetanf tfgdtitykc lpgylmfgqp nmrclangkw srtfskcsrl
     2221 scnkpkfptg vtvrgrsyly qdqltyicpg skkqglitcq angqwsdppi cdki
//
LOCUS       XP_034195865            2243 aa            linear   INV 03-SEP-2025
DEFINITION  mediator complex subunit kohtalo [Osmia lignaria lignaria].
ACCESSION   XP_034195865
VERSION     XP_034195865.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339974.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2243
                     /product="mediator complex subunit kohtalo"
                     /calculated_mol_wt=249348
     Region          97..157
                     /region_name="Med12"
                     /note="Transcription mediator complex subunit Med12;
                     pfam09497"
                     /db_xref="CDD:462818"
     Region          336..804
                     /region_name="Med12-LCEWAV"
                     /note="Eukaryotic Mediator 12 subunit domain; pfam12145"
                     /db_xref="CDD:463472"
     Region          1798..>2010
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     CDS             1..2243
                     /gene="kto"
                     /coded_by="XM_034339974.2:352..7083"
                     /db_xref="GeneID:117611759"
ORIGIN      
        1 mmgilyekrp lkrprlgppd vypqepkqke delssinvkh gfatmpqlsd efgtarhcnv
       61 taakvgayfn ailakkeels tmpdtgrkrq qinpkdnfwp vtartkngie awfkdlsgck
      121 plialakkap nfnkkeeifm mlceyqvpml raawfiklss aytvavseak ikkrqlpdpt
      181 tewtgtlikf lkdqlsklqe yyylnnyitn sisnngiann scnsngtgsn nsnnnsnsnv
      241 nnnttnnngv vnnqpatpns snqvttgnvm neehklalkq whycvqlaky mfeeglldrq
      301 ellqwilell dkiksspled gilklllpla lqyleefvqs ellarrlayl ccrklahmcs
      361 nvetngnpqs psitksevng gkdtatashv qnpltaafnd ylscphhrdv iyslstiiqv
      421 itlecptalv wnsvgegkap svlngspldy lpcppttlps ppatttnptl kqlkvaqeni
      481 rarsqaaegk wscdkwqqss agitttkvla aldaldrhsf drmdstnsld tlygkiftsp
      541 pkdttneret kpeynpqqds avveilcewa vsaerwgehr amavakllek rqsevtgetn
      601 dnddkdsics ngsppvlpif qpllmkfldm dapvldnttt qskvqftnlv hlfselirhd
      661 vfshdaymct lisrgdliqg paaskpgtps nrepidedsl fpgidlkptk levtdhgrtm
      721 dyddskiddd ldkllqhike dqqnsmdapd spkedalagh gtqegldskm psshsrhlly
      781 tthfplpqde tcsqhdcnqr hvllygvgrv rdearhivkk mtkevcklfg kkfsidvaeg
      841 gkvkkhsrse fnfeaitlkf qnlsyfdqhv vtwqcatqvi emlntfalag ssylpvqehv
      901 aflfdlmela lniyglidvc iqilkelpev eaqltvrnsq lvrsyttsls lyvvgvlrry
      961 hcclllspeq ttavfdllck vvkhvsnpsd cssaercvla hlydlysscs llktkphgve
     1021 afsnaypkir talystlqpt tsshvynsqf mvdvftsprr ggkiepqwar qlnetpanry
     1081 sfvcnaivav csetdndkln diaitcaelt accnalnaew lgvlmalccs sgssafyidv
     1141 lnqvdvqdls ihnslavfts iliarhcfsl edfvvhialp slvkacnegr gdadmeaeag
     1201 arltchlllr lfktvecpqp alysvstsph plpngnsrgy siklscdrhl laaahnnirv
     1261 gpvlavlkai lvvadatagk qppkkpdvpm nhsnkaggpa svgvgvgvnt ggsselsish
     1321 ilgtsdilgg gddlgldlam sssnssagmt tenvkglsdf aqhvlrqics qewvlerclq
     1381 npeelchpdm lldnmltprq aqrllhmicy petsidafid qkthitnile nleqwslrms
     1441 wldlqlmykq fppgssdlsq wldtvakaai dvfqlntmss ksdkrsgsiw lvaplvsklp
     1501 savqgrvlkv agqvlesgnw sktaagrers rskspslfnh qpflslvltc lkgqddqreg
     1561 llmslhsqlt qflnitkeek nfatedpktr evlqdalqlr fslvggvfdt iqrnttattd
     1621 waillvqlvs ygvidlnnnf elfttvidml atlihstlvs dsqsekdenk khyqnlmkkl
     1681 kkelgdrnsp sikyvrqllp lpkltmevit cepvgcltdt kgnkiagfds idkkqglqvc
     1741 dsqrvsawev leghknpapi swawfravrl erkpltyqna hkllryhths qirppshyld
     1801 ppplpaedle pdkkesepgk adtpmsidsp grvpssvggn aigtavtngk gkamkskrhr
     1861 rnkgaatptt pvsqqiqqpa nqlqqiafgn qqvpvsqqpg mftgqppqhq qawyanqqth
     1921 apaqqygygq qlpptpvgpr ydrpgmnnqs kqalshmlrl rlpnnqliss qqqpnaapvg
     1981 gsgfqgmqrq qfirqqlraq hgapninpqq gmfasqqqqq qpqqqgiytg mqqgmnqnya
     2041 gyggqqmipq qqqqqqqqqa pqqaqqqqql lqqqqqqqgl mnqqqnmmft nqqqimgpqr
     2101 gqeyipqrlq pgatrppylq apnvtmntmg pmaggvqnqp appyrqtsgk pgtvgvtgvg
     2161 tasvglqqnq qfqqqqainq qrmrqqmlam qqqqqqaqqq qvqqqqqqqg naggqqptpq
     2221 lvthlqrhls qppqhyqhqp ppy
//
LOCUS       XP_076546604            1597 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2 alpha kinase Gcn2
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546604
VERSION     XP_076546604.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690489.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1597
                     /product="eukaryotic translation initiation factor 2 alpha
                     kinase Gcn2 isoform X1"
                     /calculated_mol_wt=184005
     Region          7..127
                     /region_name="RWD_GCN2"
                     /note="RWD domain of eIF-2-alpha kinase GCN2 and related
                     proteins; cd23823"
                     /db_xref="CDD:467659"
     Region          267..495
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(267..269,272,274,318,338..341,384,388..389,391,
                     401..402)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          538..947
                     /region_name="STKc_EIF2AK4_GCN2_rpt2"
                     /note="Catalytic domain, repeat 2, of the Serine/Threonine
                     kinase, eukaryotic translation Initiation Factor 2-Alpha
                     Kinase 4 or General Control Non-derepressible-2; cd14046"
                     /db_xref="CDD:270948"
     Site            order(538..539,542..543,589..590,593..594,596..597,599,
                     605..613,738..739,779,782..784)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270948"
     Site            order(550..551,556,558,571,573,743..746,750,793..794,796,
                     807,852..854,889,891..898)
                     /site_type="active"
                     /db_xref="CDD:270948"
     Site            order(550..551,558,571,573,604,743..746,789,791,794,796,
                     807)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270948"
     Site            order(806..815,829..837,843..854)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270948"
     Site            order(852..854,889,891..898)
                     /site_type="other"
                     /note="eIF2alpha (substrate) binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270948"
     Region          <1021..1444
                     /region_name="PLN02972"
                     /note="Histidyl-tRNA synthetase"
                     /db_xref="CDD:215525"
     CDS             1..1597
                     /gene="Gcn2"
                     /coded_by="XM_076690489.1:311..5104"
                     /db_xref="GeneID:117610229"
ORIGIN      
        1 mscesfqdrq eneievlksi fgdelcdlrn rknrrkwqpl dvnitltpqk gmsgpakvyv
       61 qidlrvtcsd rypvevpkiv lkngrglsdq qiavlydelm eltkrlqgev milelaqhvq
      121 kylyennkrr ynsfyeemis rhqekikyem lekqlkedke rqvlqdevqk rqealkaelr
      181 nrreslrlfs dasnnsfqvi plssqeksri ysrrrcvsts etldgvlceh tgtkllhfdh
      241 skddrevyra rcmghstqgs vvyagvdttt gelfaisewm lkttnksnes ntqhvikqig
      301 sleqeinhlh klhhpnlvhy lnmkylqdge tiviyilqef vvgtscsffl lesipvdidl
      361 lrylatgils alrylhennv vhkdlrdtsi yidrtglvkl ldyslnkrls diyqsctaik
      421 svpdfpsiqs rggkkadiyr fgalmfslln givvsgdqid ptvikqpdlq dfllkclind
      481 erkrwsaeql lhhsfiktpl ihalsppkip rrdeqesqep eepdtdirqy vpllgghsri
      541 tnefeildwl grgafgdvlk vknkldggvy aikrielnpk nkqlnrkitr evkllsrmnh
      601 envvryynsw iesctitnka eeqssektss ekkspnllnh lgmddiekla ppmqevewnv
      661 syksrtnatl sadsdddnsd assdsdsded csflmqnilk idssdniefe rdtncqttis
      721 siredengte etvkeiqfmy iqmefcekst lrtaidggly kdlkrvwrlf reiveglahi
      781 hqqgmihrdl kpmnvfldsn dhvkigdfgl attnilstfv qtietdkesq gldkrisfgt
      841 ddvgsltgqv gtalyvapel ttkaakaiyn qkvdiyslgi ilfemcykpl ttgmerikvl
      901 lnlrskeiil psemqqtdms rqthilrwll nhdpsqrpta qellsseylp pppleetelq
      961 emirrtlsnn qskaykylis ccfmqevspa dditydmnvp srghmnfltw rkhqegvkck
     1021 vieifqkhgg vylgtpllmp kshqfcsfsn ssvklmtrng nivciphdlr apfaryivwn
     1081 niphirryai ervfrekkvl gfhprelyec afdiisptpd nllmeaeliy ivweiinels
     1141 lsqernftic lnhtsllqav lmycgidqek yqdiysilrd ardgkfsrfq iqthlislcl
     1201 tdqametlfn lfesessltk ihsilktiak rkgdaatlak eglkeiervm eniealgvkw
     1261 slvivpllvh ninqhsgiiy qitynvkrrr rkcgeeviaa ggrydkmlss fkkilertgm
     1321 tskeikqyga gisvslekli svvsettesv eskhgmyvai scignsyrek emidllkelw
     1381 slglkvtild lasveeileh crenlinhvi llksgekgcv riqswerdrf qekkvtnqei
     1441 geffqrldns mpilnrsesk titndsclsn nspvnininf isseksekvs gsskrslkns
     1501 ilaqmstyfq rishkvpiei favsldmsvi rtiicfleid ekehdflksv qliiekhpkh
     1561 kkyimeicee mqearkekqr palilyslmd nqymtll
//
LOCUS       XP_076546605            1597 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2 alpha kinase Gcn2
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546605
VERSION     XP_076546605.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690490.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1597
                     /product="eukaryotic translation initiation factor 2 alpha
                     kinase Gcn2 isoform X1"
                     /calculated_mol_wt=184005
     Region          7..127
                     /region_name="RWD_GCN2"
                     /note="RWD domain of eIF-2-alpha kinase GCN2 and related
                     proteins; cd23823"
                     /db_xref="CDD:467659"
     Region          267..495
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(267..269,272,274,318,338..341,384,388..389,391,
                     401..402)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          538..947
                     /region_name="STKc_EIF2AK4_GCN2_rpt2"
                     /note="Catalytic domain, repeat 2, of the Serine/Threonine
                     kinase, eukaryotic translation Initiation Factor 2-Alpha
                     Kinase 4 or General Control Non-derepressible-2; cd14046"
                     /db_xref="CDD:270948"
     Site            order(538..539,542..543,589..590,593..594,596..597,599,
                     605..613,738..739,779,782..784)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270948"
     Site            order(550..551,556,558,571,573,743..746,750,793..794,796,
                     807,852..854,889,891..898)
                     /site_type="active"
                     /db_xref="CDD:270948"
     Site            order(550..551,558,571,573,604,743..746,789,791,794,796,
                     807)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270948"
     Site            order(806..815,829..837,843..854)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270948"
     Site            order(852..854,889,891..898)
                     /site_type="other"
                     /note="eIF2alpha (substrate) binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270948"
     Region          <1021..1444
                     /region_name="PLN02972"
                     /note="Histidyl-tRNA synthetase"
                     /db_xref="CDD:215525"
     CDS             1..1597
                     /gene="Gcn2"
                     /coded_by="XM_076690490.1:227..5020"
                     /db_xref="GeneID:117610229"
ORIGIN      
        1 mscesfqdrq eneievlksi fgdelcdlrn rknrrkwqpl dvnitltpqk gmsgpakvyv
       61 qidlrvtcsd rypvevpkiv lkngrglsdq qiavlydelm eltkrlqgev milelaqhvq
      121 kylyennkrr ynsfyeemis rhqekikyem lekqlkedke rqvlqdevqk rqealkaelr
      181 nrreslrlfs dasnnsfqvi plssqeksri ysrrrcvsts etldgvlceh tgtkllhfdh
      241 skddrevyra rcmghstqgs vvyagvdttt gelfaisewm lkttnksnes ntqhvikqig
      301 sleqeinhlh klhhpnlvhy lnmkylqdge tiviyilqef vvgtscsffl lesipvdidl
      361 lrylatgils alrylhennv vhkdlrdtsi yidrtglvkl ldyslnkrls diyqsctaik
      421 svpdfpsiqs rggkkadiyr fgalmfslln givvsgdqid ptvikqpdlq dfllkclind
      481 erkrwsaeql lhhsfiktpl ihalsppkip rrdeqesqep eepdtdirqy vpllgghsri
      541 tnefeildwl grgafgdvlk vknkldggvy aikrielnpk nkqlnrkitr evkllsrmnh
      601 envvryynsw iesctitnka eeqssektss ekkspnllnh lgmddiekla ppmqevewnv
      661 syksrtnatl sadsdddnsd assdsdsded csflmqnilk idssdniefe rdtncqttis
      721 siredengte etvkeiqfmy iqmefcekst lrtaidggly kdlkrvwrlf reiveglahi
      781 hqqgmihrdl kpmnvfldsn dhvkigdfgl attnilstfv qtietdkesq gldkrisfgt
      841 ddvgsltgqv gtalyvapel ttkaakaiyn qkvdiyslgi ilfemcykpl ttgmerikvl
      901 lnlrskeiil psemqqtdms rqthilrwll nhdpsqrpta qellsseylp pppleetelq
      961 emirrtlsnn qskaykylis ccfmqevspa dditydmnvp srghmnfltw rkhqegvkck
     1021 vieifqkhgg vylgtpllmp kshqfcsfsn ssvklmtrng nivciphdlr apfaryivwn
     1081 niphirryai ervfrekkvl gfhprelyec afdiisptpd nllmeaeliy ivweiinels
     1141 lsqernftic lnhtsllqav lmycgidqek yqdiysilrd ardgkfsrfq iqthlislcl
     1201 tdqametlfn lfesessltk ihsilktiak rkgdaatlak eglkeiervm eniealgvkw
     1261 slvivpllvh ninqhsgiiy qitynvkrrr rkcgeeviaa ggrydkmlss fkkilertgm
     1321 tskeikqyga gisvslekli svvsettesv eskhgmyvai scignsyrek emidllkelw
     1381 slglkvtild lasveeileh crenlinhvi llksgekgcv riqswerdrf qekkvtnqei
     1441 geffqrldns mpilnrsesk titndsclsn nspvnininf isseksekvs gsskrslkns
     1501 ilaqmstyfq rishkvpiei favsldmsvi rtiicfleid ekehdflksv qliiekhpkh
     1561 kkyimeicee mqearkekqr palilyslmd nqymtll
//
LOCUS       XP_034193262            1586 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2 alpha kinase Gcn2
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193262
VERSION     XP_034193262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1586
                     /product="eukaryotic translation initiation factor 2 alpha
                     kinase Gcn2 isoform X2"
                     /calculated_mol_wt=182740
     Region          7..127
                     /region_name="RWD_GCN2"
                     /note="RWD domain of eIF-2-alpha kinase GCN2 and related
                     proteins; cd23823"
                     /db_xref="CDD:467659"
     Region          256..484
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(256..258,261,263,307,327..330,373,377..378,380,
                     390..391)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          527..936
                     /region_name="STKc_EIF2AK4_GCN2_rpt2"
                     /note="Catalytic domain, repeat 2, of the Serine/Threonine
                     kinase, eukaryotic translation Initiation Factor 2-Alpha
                     Kinase 4 or General Control Non-derepressible-2; cd14046"
                     /db_xref="CDD:270948"
     Site            order(527..528,531..532,578..579,582..583,585..586,588,
                     594..602,727..728,768,771..773)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270948"
     Site            order(539..540,545,547,560,562,732..735,739,782..783,785,
                     796,841..843,878,880..887)
                     /site_type="active"
                     /db_xref="CDD:270948"
     Site            order(539..540,547,560,562,593,732..735,778,780,783,785,
                     796)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270948"
     Site            order(795..804,818..826,832..843)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270948"
     Site            order(841..843,878,880..887)
                     /site_type="other"
                     /note="eIF2alpha (substrate) binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270948"
     Region          <1010..1433
                     /region_name="PLN02972"
                     /note="Histidyl-tRNA synthetase"
                     /db_xref="CDD:215525"
     CDS             1..1586
                     /gene="Gcn2"
                     /coded_by="XM_034337371.2:302..5062"
                     /db_xref="GeneID:117610229"
ORIGIN      
        1 mscesfqdrq eneievlksi fgdelcdlrn rknrrkwqpl dvnitltpqk gmsgpakvyv
       61 qidlrvtcsd rypvevpkiv lkngrglsdq qiavlydelm eltkrlqgev milelaqhvq
      121 kylyennkrr ynsfyeemis rhqekikyem lekqlkedke rqvlqdevqk rqealkaelr
      181 nrreslrlfs dasnnsfqvi plssqeksri ysrrrcvsts etldgvlceh tddrevyrar
      241 cmghstqgsv vyagvdtttg elfaisewml kttnksnesn tqhvikqigs leqeinhlhk
      301 lhhpnlvhyl nmkylqdget iviyilqefv vgtscsffll esipvdidll rylatgilsa
      361 lrylhennvv hkdlrdtsiy idrtglvkll dyslnkrlsd iyqsctaiks vpdfpsiqsr
      421 ggkkadiyrf galmfsllng ivvsgdqidp tvikqpdlqd fllkclinde rkrwsaeqll
      481 hhsfiktpli halsppkipr rdeqesqepe epdtdirqyv pllgghsrit nefeildwlg
      541 rgafgdvlkv knkldggvya ikrielnpkn kqlnrkitre vkllsrmnhe nvvryynswi
      601 esctitnkae eqssektsse kkspnllnhl gmddieklap pmqevewnvs yksrtnatls
      661 adsdddnsda ssdsdsdedc sflmqnilki dssdniefer dtncqttiss iredengtee
      721 tvkeiqfmyi qmefcekstl rtaidgglyk dlkrvwrlfr eiveglahih qqgmihrdlk
      781 pmnvfldsnd hvkigdfgla ttnilstfvq tietdkesqg ldkrisfgtd dvgsltgqvg
      841 talyvapelt tkaakaiynq kvdiyslgii lfemcykplt tgmerikvll nlrskeiilp
      901 semqqtdmsr qthilrwlln hdpsqrptaq ellsseylpp ppleetelqe mirrtlsnnq
      961 skaykylisc cfmqevspad ditydmnvps rghmnfltwr khqegvkckv ieifqkhggv
     1021 ylgtpllmpk shqfcsfsns svklmtrngn ivciphdlra pfaryivwnn iphirryaie
     1081 rvfrekkvlg fhprelyeca fdiisptpdn llmeaeliyi vweiinelsl sqernfticl
     1141 nhtsllqavl mycgidqeky qdiysilrda rdgkfsrfqi qthlislclt dqametlfnl
     1201 fesessltki hsilktiakr kgdaatlake glkeiervme niealgvkws lvivpllvhn
     1261 inqhsgiiyq itynvkrrrr kcgeeviaag grydkmlssf kkilertgmt skeikqygag
     1321 isvsleklis vvsettesve skhgmyvais cignsyreke midllkelws lglkvtildl
     1381 asveeilehc renlinhvil lksgekgcvr iqswerdrfq ekkvtnqeig effqrldnsm
     1441 pilnrseskt itndsclsnn spvnininfi sseksekvsg sskrslknsi laqmstyfqr
     1501 ishkvpieif avsldmsvir tiicfleide kehdflksvq liiekhpkhk kyimeiceem
     1561 qearkekqrp alilyslmdn qymtll
//
LOCUS       XP_034193272            1573 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2 alpha kinase Gcn2
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034193272
VERSION     XP_034193272.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337381.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193272.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1573
                     /product="eukaryotic translation initiation factor 2 alpha
                     kinase Gcn2 isoform X3"
                     /calculated_mol_wt=181329
     Region          7..127
                     /region_name="RWD_GCN2"
                     /note="RWD domain of eIF-2-alpha kinase GCN2 and related
                     proteins; cd23823"
                     /db_xref="CDD:467659"
     Region          267..495
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(267..269,272,274,318,338..341,384,388..389,391,
                     401..402)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          538..947
                     /region_name="STKc_EIF2AK4_GCN2_rpt2"
                     /note="Catalytic domain, repeat 2, of the Serine/Threonine
                     kinase, eukaryotic translation Initiation Factor 2-Alpha
                     Kinase 4 or General Control Non-derepressible-2; cd14046"
                     /db_xref="CDD:270948"
     Site            order(538..539,542..543,589..590,593..594,596..597,599,
                     605..613,738..739,779,782..784)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270948"
     Site            order(550..551,556,558,571,573,743..746,750,793..794,796,
                     807,852..854,889,891..898)
                     /site_type="active"
                     /db_xref="CDD:270948"
     Site            order(550..551,558,571,573,604,743..746,789,791,794,796,
                     807)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270948"
     Site            order(806..815,829..837,843..854)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270948"
     Site            order(852..854,889,891..898)
                     /site_type="other"
                     /note="eIF2alpha (substrate) binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270948"
     Region          <1031..1420
                     /region_name="PLN02972"
                     /note="Histidyl-tRNA synthetase"
                     /db_xref="CDD:215525"
     CDS             1..1573
                     /gene="Gcn2"
                     /coded_by="XM_034337381.2:302..5023"
                     /db_xref="GeneID:117610229"
ORIGIN      
        1 mscesfqdrq eneievlksi fgdelcdlrn rknrrkwqpl dvnitltpqk gmsgpakvyv
       61 qidlrvtcsd rypvevpkiv lkngrglsdq qiavlydelm eltkrlqgev milelaqhvq
      121 kylyennkrr ynsfyeemis rhqekikyem lekqlkedke rqvlqdevqk rqealkaelr
      181 nrreslrlfs dasnnsfqvi plssqeksri ysrrrcvsts etldgvlceh tgtkllhfdh
      241 skddrevyra rcmghstqgs vvyagvdttt gelfaisewm lkttnksnes ntqhvikqig
      301 sleqeinhlh klhhpnlvhy lnmkylqdge tiviyilqef vvgtscsffl lesipvdidl
      361 lrylatgils alrylhennv vhkdlrdtsi yidrtglvkl ldyslnkrls diyqsctaik
      421 svpdfpsiqs rggkkadiyr fgalmfslln givvsgdqid ptvikqpdlq dfllkclind
      481 erkrwsaeql lhhsfiktpl ihalsppkip rrdeqesqep eepdtdirqy vpllgghsri
      541 tnefeildwl grgafgdvlk vknkldggvy aikrielnpk nkqlnrkitr evkllsrmnh
      601 envvryynsw iesctitnka eeqssektss ekkspnllnh lgmddiekla ppmqevewnv
      661 syksrtnatl sadsdddnsd assdsdsded csflmqnilk idssdniefe rdtncqttis
      721 siredengte etvkeiqfmy iqmefcekst lrtaidggly kdlkrvwrlf reiveglahi
      781 hqqgmihrdl kpmnvfldsn dhvkigdfgl attnilstfv qtietdkesq gldkrisfgt
      841 ddvgsltgqv gtalyvapel ttkaakaiyn qkvdiyslgi ilfemcykpl ttgmerikvl
      901 lnlrskeiil psemqqtdms rqthilrwll nhdpsqrpta qellsseylp pppleetelq
      961 emirrtlsnn qskaykylis ccfmqevspa dditydmnvp srghmnfltw rkhqegvkck
     1021 fcsfsnssvk lmtrngnivc iphdlrapfa ryivwnniph irryaiervf rekkvlgfhp
     1081 relyecafdi isptpdnllm eaeliyivwe iinelslsqe rnfticlnht sllqavlmyc
     1141 gidqekyqdi ysilrdardg kfsrfqiqth lislcltdqa metlfnlfes essltkihsi
     1201 lktiakrkgd aatlakeglk eiervmenie algvkwslvi vpllvhninq hsgiiyqity
     1261 nvkrrrrkcg eeviaaggry dkmlssfkki lertgmtske ikqygagisv sleklisvvs
     1321 ettesveskh gmyvaiscig nsyrekemid llkelwslgl kvtildlasv eeilehcren
     1381 linhvillks gekgcvriqs werdrfqekk vtnqeigeff qrldnsmpil nrsesktitn
     1441 dsclsnnspv nininfisse ksekvsgssk rslknsilaq mstyfqrish kvpieifavs
     1501 ldmsvirtii cfleidekeh dflksvqlii ekhpkhkkyi meiceemqea rkekqrpali
     1561 lyslmdnqym tll
//
LOCUS       XP_034193285            1408 aa            linear   INV 03-SEP-2025
DEFINITION  Bloom syndrome helicase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193285
VERSION     XP_034193285.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337394.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193285.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1408
                     /product="Bloom syndrome helicase isoform X1"
                     /calculated_mol_wt=159498
     Region          670..1161
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     Region          1230..1296
                     /region_name="HRDC"
                     /note="HRDC domain; pfam00570"
                     /db_xref="CDD:425755"
     CDS             1..1408
                     /gene="Blm"
                     /coded_by="XM_034337394.2:277..4503"
                     /db_xref="GeneID:117610242"
ORIGIN      
        1 mskkiplsqn diqptfkgfl ermknkqmqs stpkasninl qlnegldmck nhesdnvkks
       61 evntkssiis ldssneslcd ssniikksdn qdakqeetls nsicnptfst ientqksktk
      121 viyssdsedd irvkptssln rknrifnssn eesiinehsp svnkdcrsai ndvsskespd
      181 nnnvyetein ekkligddsp rtprkystdi knenfiisds dsspsfkhsp iqisrkkwig
      241 pdfklnlkpl glgkqldpwi essrkkpims svpvtkqkle drykhlkdfq ievlekfyaa
      301 ldqiplpvle kfpdfnkhsf qnlkilyqhv kakirlvetk lihvktdene rpaddklcei
      361 knssplskii gtsggqsils isdssscnls tvnlvdtess sintmkment vspivkgssq
      421 ffekknesqv keqkdtiins ygspnqvkst dslvqgkksk fqlkrpikat igsevskrig
      481 eiwekeeqsk ivnpkavavn sdnyinfddy kakqieemwe kdkqskivns kavtvnsdny
      541 tnfdnyeakr ieinkdacyt eekkqdlrss eilkcennqq dwfdtlnvdd dalysedivd
      601 cltqeleqfp alnkecniks vvatqnindn eygkeqqkta ktnteigift gnykndgvsg
      661 efdgltyphs remlkifrqk fglysfrpnq lqainaamlg fdcfvlmptg ggkslcyqlp
      721 alllrgitiv isplkslild qvqkltsldi paahmsgtit dsqaegiyre lskkepalkl
      781 lyvtpekisa sqkfcnlltt lyerellarf videahcvsq wghdfrpdyk rlkclrdkyp
      841 kvpmialtat atprvrtdil yqlgltqpkw fmssfnrpnl rysiiakkgk ncsdeviami
      901 ntkykndcgi vyclsrkecd dyamqmkkng ikalsyhagl tdnnrsniqg rwiseeikvv
      961 catiafgmgi dkpnvrfvih aalpksiegy yqesgragrd genadcilfy nyvdmhrirk
     1021 miemdnsnpd aiktnidnlf kmvsfcenkt dcrrtqqlny fgevfnrqqc iankptscdn
     1081 crckddftml natedakqim kavrdinqsr scnltlvllt difkgsdlkk iretglnklp
     1141 lygrgrswnr sdierllhnm vlkdylqetm yinneiacay lkigpnanel mtrkdikvei
     1201 pmkssnnssn avatvstvtk kidkvllelq ercykelmei ikgiagaldi saasvmnmia
     1261 iramsqklpd teeamlqiph vtkanfekyg kallditqky aaekyvllse eqaesngsdn
     1321 dddgtwdndt ssftsnfnkr rgtkrkgksn yrntmkkfkr tttsttqkgk tttkgknaas
     1381 ssskgpglvs lntkkdyssn pyrymqfg
//
LOCUS       XP_076546606            1408 aa            linear   INV 03-SEP-2025
DEFINITION  Bloom syndrome helicase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546606
VERSION     XP_076546606.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690491.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1408
                     /product="Bloom syndrome helicase isoform X1"
                     /calculated_mol_wt=159498
     Region          670..1161
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     Region          1230..1296
                     /region_name="HRDC"
                     /note="HRDC domain; pfam00570"
                     /db_xref="CDD:425755"
     CDS             1..1408
                     /gene="Blm"
                     /coded_by="XM_076690491.1:36..4262"
                     /db_xref="GeneID:117610242"
ORIGIN      
        1 mskkiplsqn diqptfkgfl ermknkqmqs stpkasninl qlnegldmck nhesdnvkks
       61 evntkssiis ldssneslcd ssniikksdn qdakqeetls nsicnptfst ientqksktk
      121 viyssdsedd irvkptssln rknrifnssn eesiinehsp svnkdcrsai ndvsskespd
      181 nnnvyetein ekkligddsp rtprkystdi knenfiisds dsspsfkhsp iqisrkkwig
      241 pdfklnlkpl glgkqldpwi essrkkpims svpvtkqkle drykhlkdfq ievlekfyaa
      301 ldqiplpvle kfpdfnkhsf qnlkilyqhv kakirlvetk lihvktdene rpaddklcei
      361 knssplskii gtsggqsils isdssscnls tvnlvdtess sintmkment vspivkgssq
      421 ffekknesqv keqkdtiins ygspnqvkst dslvqgkksk fqlkrpikat igsevskrig
      481 eiwekeeqsk ivnpkavavn sdnyinfddy kakqieemwe kdkqskivns kavtvnsdny
      541 tnfdnyeakr ieinkdacyt eekkqdlrss eilkcennqq dwfdtlnvdd dalysedivd
      601 cltqeleqfp alnkecniks vvatqnindn eygkeqqkta ktnteigift gnykndgvsg
      661 efdgltyphs remlkifrqk fglysfrpnq lqainaamlg fdcfvlmptg ggkslcyqlp
      721 alllrgitiv isplkslild qvqkltsldi paahmsgtit dsqaegiyre lskkepalkl
      781 lyvtpekisa sqkfcnlltt lyerellarf videahcvsq wghdfrpdyk rlkclrdkyp
      841 kvpmialtat atprvrtdil yqlgltqpkw fmssfnrpnl rysiiakkgk ncsdeviami
      901 ntkykndcgi vyclsrkecd dyamqmkkng ikalsyhagl tdnnrsniqg rwiseeikvv
      961 catiafgmgi dkpnvrfvih aalpksiegy yqesgragrd genadcilfy nyvdmhrirk
     1021 miemdnsnpd aiktnidnlf kmvsfcenkt dcrrtqqlny fgevfnrqqc iankptscdn
     1081 crckddftml natedakqim kavrdinqsr scnltlvllt difkgsdlkk iretglnklp
     1141 lygrgrswnr sdierllhnm vlkdylqetm yinneiacay lkigpnanel mtrkdikvei
     1201 pmkssnnssn avatvstvtk kidkvllelq ercykelmei ikgiagaldi saasvmnmia
     1261 iramsqklpd teeamlqiph vtkanfekyg kallditqky aaekyvllse eqaesngsdn
     1321 dddgtwdndt ssftsnfnkr rgtkrkgksn yrntmkkfkr tttsttqkgk tttkgknaas
     1381 ssskgpglvs lntkkdyssn pyrymqfg
//
LOCUS       XP_034193295            1407 aa            linear   INV 03-SEP-2025
DEFINITION  Bloom syndrome helicase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193295
VERSION     XP_034193295.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337404.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193295.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1407
                     /product="Bloom syndrome helicase isoform X2"
                     /calculated_mol_wt=159399
     Region          669..1160
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     Region          1229..1295
                     /region_name="HRDC"
                     /note="HRDC domain; pfam00570"
                     /db_xref="CDD:425755"
     CDS             1..1407
                     /gene="Blm"
                     /coded_by="XM_034337404.2:277..4500"
                     /db_xref="GeneID:117610242"
ORIGIN      
        1 mskkiplsqn diqptfkgfl ermknkqmqs stpkasninl qlnegldmck nhesdnvkks
       61 evntkssiis ldssneslcd ssniikksdn qdakqeetls nsicnptfst ientqksktk
      121 viyssdsedd irvkptssln rknrifnssn eesiinehsp svnkdcrsai ndvsskespd
      181 nnnvyetein ekkligddsp rtprkystdi knenfiisds dsspsfkhsp iqisrkkwig
      241 pdfklnlkpl glgkqldpwi essrkkpims svpvtkqkle drykhlkdfq ievlekfyaa
      301 ldqiplpvle kfpdfnkhsf qnlkilyqhv kakirlvetk lihvktdene rpaddklcei
      361 knssplskii gtsggqsils isdssscnls tvnlvdtess sintmkment vspivkgssq
      421 ffekknesqv keqkdtiins ygspnqvkst dslvqgkksk fqlkrpikat igsevskrig
      481 eiwekeeqsk ivnpkavavn sdnyinfddy kakqieemwe kdkqskivns kavtvnsdny
      541 tnfdnyeakr ieinkdacyt eekkqdlrss eilkcennqq dwfdtlnvdd dalysedivd
      601 cltqeleqfp alnkecniks vatqnindne ygkeqqktak tnteigiftg nykndgvsge
      661 fdgltyphsr emlkifrqkf glysfrpnql qainaamlgf dcfvlmptgg gkslcyqlpa
      721 lllrgitivi splkslildq vqkltsldip aahmsgtitd sqaegiyrel skkepalkll
      781 yvtpekisas qkfcnllttl yerellarfv ideahcvsqw ghdfrpdykr lkclrdkypk
      841 vpmialtata tprvrtdily qlgltqpkwf mssfnrpnlr ysiiakkgkn csdeviamin
      901 tkykndcgiv yclsrkecdd yamqmkkngi kalsyhaglt dnnrsniqgr wiseeikvvc
      961 atiafgmgid kpnvrfviha alpksiegyy qesgragrdg enadcilfyn yvdmhrirkm
     1021 iemdnsnpda iktnidnlfk mvsfcenktd crrtqqlnyf gevfnrqqci ankptscdnc
     1081 rckddftmln atedakqimk avrdinqsrs cnltlvlltd ifkgsdlkki retglnklpl
     1141 ygrgrswnrs dierllhnmv lkdylqetmy inneiacayl kigpnanelm trkdikveip
     1201 mkssnnssna vatvstvtkk idkvllelqe rcykelmeii kgiagaldis aasvmnmiai
     1261 ramsqklpdt eeamlqiphv tkanfekygk allditqkya aekyvllsee qaesngsdnd
     1321 ddgtwdndts sftsnfnkrr gtkrkgksny rntmkkfkrt ttsttqkgkt ttkgknaass
     1381 sskgpglvsl ntkkdyssnp yrymqfg
//
LOCUS       XP_034193308            1040 aa            linear   INV 03-SEP-2025
DEFINITION  mutagen-sensitive 301 [Osmia lignaria lignaria].
ACCESSION   XP_034193308
VERSION     XP_034193308.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337417.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193308.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1040
                     /product="mutagen-sensitive 301"
                     /calculated_mol_wt=118329
     Region          248..738
                     /region_name="BRR2"
                     /note="Replicative superfamily II helicase [Replication,
                     recombination and repair]; COG1204"
                     /db_xref="CDD:440817"
     Region          956..1011
                     /region_name="HHH_5"
                     /note="Helix-hairpin-helix domain; pfam14520"
                     /db_xref="CDD:434010"
     CDS             1..1040
                     /gene="mus301"
                     /coded_by="XM_034337417.2:51..3173"
                     /db_xref="GeneID:117610249"
ORIGIN      
        1 myictikdkl fmivdiiimp ntnsflnnsh vnvnkssegq yeivskslqv idsilsmddt
       61 ilrsieddea lvspemhkkt ldnfthkwrd tcesneklev sdksknnvqi eareentlsi
      121 lhgispeann hwldntflne vftqlessnk endamehciq vatetfenys gesafmenih
      181 tsnkehqnvk llkrnliqyn nvipskvhcs ndkkinrwsi dtnhstsikd dtfyglsntv
      241 kklfsqirgi ntlyqwqdec lsldairnrk nliyalptsg gktlvaeilm lkeiicnkrn
      301 aifilpfvai vqekvqamap faleldflie eyaaskgvyp pkkrrkkksi ymctiekalg
      361 linslieekr fneigtiivd elhllgesgg rgatlevila ktlyvnenih ivgmsatign
      421 lneiaeflna dvytknfrpv eikeyvkced niwlfdptae eiltdvkkin yryssdaafi
      481 dpdrlgglvm dvipqdscli fcssrkncen vallltrilf ksleihkkde kqnllnalks
      541 eeglcpilqk titfgvayhh sgltteerrl ledafkvgtl cvicctstla agvnlparrv
      601 ilrcpyvgnq fvnlsrykqm tgragrtgmr nigesilick rdelskvkel leskmddcls
      661 tlhvnkdrgi nnlilsalls siattrselh kiskktllni qqkrldvnik hivdttitef
      721 lksgvlkikd psrnidefkp nisvvipsqi tnsndavien kekklikirn dtklelcslg
      781 raamkgcidm qcaytlyedl kktqehlilv dylhllylvt pyditsqiki vgpvyydvvi
      841 nlsetqmkta rllginevti skirdgimpk kveprvvqrf yvtlilyelw tqhevyaiae
      901 kfqvnrgvvq nllnavssfa fsvvrfcqel nefwafqdll ntfskklsyc cpselealmd
      961 lpsvkigrar qlynagfktv qciakvqpkd lqdkiqylsk kvatqiieaa kmiilekven
     1021 lrdetedild ginmnnlkri
//
LOCUS       XP_076546607             861 aa            linear   INV 03-SEP-2025
DEFINITION  daxx-like protein [Osmia lignaria lignaria].
ACCESSION   XP_076546607
VERSION     XP_076546607.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690492.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="daxx-like protein"
                     /calculated_mol_wt=98347
     Region          273..484
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(284..285,288,291..292,294..300,302..305,307..310,
                     312..313,316,319..320,323,336..338,384..388,391,418,
                     422..423,426,429,432..433,436..437,439..441,443,468,
                     471..472,475..476,478..479,482..483)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(298,384,386,389,430,433..434,437,442,447..450,453,
                     456,460,463,467,470..471,474..475,478,482)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..861
                     /gene="Daxx"
                     /coded_by="XM_076690492.1:714..3299"
                     /db_xref="GeneID:117611760"
ORIGIN      
        1 meseiiciss dddesmehnk tnntkssvik qtkdehsssi kerstlsrne itkinkkkrk
       61 isevidenda cfknqakvim ctedsitkrd aiknsdelpt vqkdntlssd edvmllkpkl
      121 vvkekksifl eeqdifpmfi slclqkdrse dmktvvnklk rryehldpty atsetfinll
      181 nekrndimls knklyihiae vmnemknnck knsalltnga ncvtninkln tgnssaktlr
      241 sstshntvsd neemlttgek ndkdekrdgk hnsqrkirvi lkamrkceky iklleesevd
      301 fdqennsnyi klerykhriv elhqiyceci gesadagrqy lrpkhfsttg ivsvdhaits
      361 finskmskrs klkkigaftn alifpdyrdi lecvtkcnem nnlgldnkkq qqiakkafte
      421 lgeylqrsrr ndywdtfslf fenkddddpa lkdrqlaekl mqnkqegekr lanvfqeyvk
      481 kqeekndhsi dsetssenee needctened mdinnetdni sedgtslnnd dnkastdede
      541 ngidgskqsv skmdinnkps vnevqynvie irekqqtksn nklkdnvsnn nlkikqvdid
      601 enisksehtq likenetiql sdtvphnlnt spgliplttl smsdnkipie kakeddsnii
      661 neklinepps eeekpllrvr sfaklpgtwk diqekvgqld knndlidltq espkqnstah
      721 qqasiqsksk vlpttkdkrk mlvipsgkhi ikvqnvtnny mrigskssmd fnnkaklqke
      781 vvtssyqivg nssrtttirl ppnqnfnret kdnnqgnssq akkkniivqv pqaaqivlll
      841 gkqkekslqq sktnvsvsqs k
//
LOCUS       XP_034195866             861 aa            linear   INV 03-SEP-2025
DEFINITION  daxx-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034195866
VERSION     XP_034195866.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339975.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195866.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="daxx-like protein"
                     /calculated_mol_wt=98347
     Region          273..484
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(284..285,288,291..292,294..300,302..305,307..310,
                     312..313,316,319..320,323,336..338,384..388,391,418,
                     422..423,426,429,432..433,436..437,439..441,443,468,
                     471..472,475..476,478..479,482..483)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(298,384,386,389,430,433..434,437,442,447..450,453,
                     456,460,463,467,470..471,474..475,478,482)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..861
                     /gene="Daxx"
                     /coded_by="XM_034339975.2:440..3025"
                     /db_xref="GeneID:117611760"
ORIGIN      
        1 meseiiciss dddesmehnk tnntkssvik qtkdehsssi kerstlsrne itkinkkkrk
       61 isevidenda cfknqakvim ctedsitkrd aiknsdelpt vqkdntlssd edvmllkpkl
      121 vvkekksifl eeqdifpmfi slclqkdrse dmktvvnklk rryehldpty atsetfinll
      181 nekrndimls knklyihiae vmnemknnck knsalltnga ncvtninkln tgnssaktlr
      241 sstshntvsd neemlttgek ndkdekrdgk hnsqrkirvi lkamrkceky iklleesevd
      301 fdqennsnyi klerykhriv elhqiyceci gesadagrqy lrpkhfsttg ivsvdhaits
      361 finskmskrs klkkigaftn alifpdyrdi lecvtkcnem nnlgldnkkq qqiakkafte
      421 lgeylqrsrr ndywdtfslf fenkddddpa lkdrqlaekl mqnkqegekr lanvfqeyvk
      481 kqeekndhsi dsetssenee needctened mdinnetdni sedgtslnnd dnkastdede
      541 ngidgskqsv skmdinnkps vnevqynvie irekqqtksn nklkdnvsnn nlkikqvdid
      601 enisksehtq likenetiql sdtvphnlnt spgliplttl smsdnkipie kakeddsnii
      661 neklinepps eeekpllrvr sfaklpgtwk diqekvgqld knndlidltq espkqnstah
      721 qqasiqsksk vlpttkdkrk mlvipsgkhi ikvqnvtnny mrigskssmd fnnkaklqke
      781 vvtssyqivg nssrtttirl ppnqnfnret kdnnqgnssq akkkniivqv pqaaqivlll
      841 gkqkekslqq sktnvsvsqs k
//
LOCUS       XP_034195867             861 aa            linear   INV 03-SEP-2025
DEFINITION  daxx-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034195867
VERSION     XP_034195867.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339976.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195867.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="daxx-like protein"
                     /calculated_mol_wt=98347
     Region          273..484
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(284..285,288,291..292,294..300,302..305,307..310,
                     312..313,316,319..320,323,336..338,384..388,391,418,
                     422..423,426,429,432..433,436..437,439..441,443,468,
                     471..472,475..476,478..479,482..483)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(298,384,386,389,430,433..434,437,442,447..450,453,
                     456,460,463,467,470..471,474..475,478,482)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..861
                     /gene="Daxx"
                     /coded_by="XM_034339976.2:714..3299"
                     /db_xref="GeneID:117611760"
ORIGIN      
        1 meseiiciss dddesmehnk tnntkssvik qtkdehsssi kerstlsrne itkinkkkrk
       61 isevidenda cfknqakvim ctedsitkrd aiknsdelpt vqkdntlssd edvmllkpkl
      121 vvkekksifl eeqdifpmfi slclqkdrse dmktvvnklk rryehldpty atsetfinll
      181 nekrndimls knklyihiae vmnemknnck knsalltnga ncvtninkln tgnssaktlr
      241 sstshntvsd neemlttgek ndkdekrdgk hnsqrkirvi lkamrkceky iklleesevd
      301 fdqennsnyi klerykhriv elhqiyceci gesadagrqy lrpkhfsttg ivsvdhaits
      361 finskmskrs klkkigaftn alifpdyrdi lecvtkcnem nnlgldnkkq qqiakkafte
      421 lgeylqrsrr ndywdtfslf fenkddddpa lkdrqlaekl mqnkqegekr lanvfqeyvk
      481 kqeekndhsi dsetssenee needctened mdinnetdni sedgtslnnd dnkastdede
      541 ngidgskqsv skmdinnkps vnevqynvie irekqqtksn nklkdnvsnn nlkikqvdid
      601 enisksehtq likenetiql sdtvphnlnt spgliplttl smsdnkipie kakeddsnii
      661 neklinepps eeekpllrvr sfaklpgtwk diqekvgqld knndlidltq espkqnstah
      721 qqasiqsksk vlpttkdkrk mlvipsgkhi ikvqnvtnny mrigskssmd fnnkaklqke
      781 vvtssyqivg nssrtttirl ppnqnfnret kdnnqgnssq akkkniivqv pqaaqivlll
      841 gkqkekslqq sktnvsvsqs k
//
LOCUS       XP_076546608             861 aa            linear   INV 03-SEP-2025
DEFINITION  daxx-like protein [Osmia lignaria lignaria].
ACCESSION   XP_076546608
VERSION     XP_076546608.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690493.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="daxx-like protein"
                     /calculated_mol_wt=98347
     Region          273..484
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(284..285,288,291..292,294..300,302..305,307..310,
                     312..313,316,319..320,323,336..338,384..388,391,418,
                     422..423,426,429,432..433,436..437,439..441,443,468,
                     471..472,475..476,478..479,482..483)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(298,384,386,389,430,433..434,437,442,447..450,453,
                     456,460,463,467,470..471,474..475,478,482)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..861
                     /gene="Daxx"
                     /coded_by="XM_076690493.1:657..3242"
                     /db_xref="GeneID:117611760"
ORIGIN      
        1 meseiiciss dddesmehnk tnntkssvik qtkdehsssi kerstlsrne itkinkkkrk
       61 isevidenda cfknqakvim ctedsitkrd aiknsdelpt vqkdntlssd edvmllkpkl
      121 vvkekksifl eeqdifpmfi slclqkdrse dmktvvnklk rryehldpty atsetfinll
      181 nekrndimls knklyihiae vmnemknnck knsalltnga ncvtninkln tgnssaktlr
      241 sstshntvsd neemlttgek ndkdekrdgk hnsqrkirvi lkamrkceky iklleesevd
      301 fdqennsnyi klerykhriv elhqiyceci gesadagrqy lrpkhfsttg ivsvdhaits
      361 finskmskrs klkkigaftn alifpdyrdi lecvtkcnem nnlgldnkkq qqiakkafte
      421 lgeylqrsrr ndywdtfslf fenkddddpa lkdrqlaekl mqnkqegekr lanvfqeyvk
      481 kqeekndhsi dsetssenee needctened mdinnetdni sedgtslnnd dnkastdede
      541 ngidgskqsv skmdinnkps vnevqynvie irekqqtksn nklkdnvsnn nlkikqvdid
      601 enisksehtq likenetiql sdtvphnlnt spgliplttl smsdnkipie kakeddsnii
      661 neklinepps eeekpllrvr sfaklpgtwk diqekvgqld knndlidltq espkqnstah
      721 qqasiqsksk vlpttkdkrk mlvipsgkhi ikvqnvtnny mrigskssmd fnnkaklqke
      781 vvtssyqivg nssrtttirl ppnqnfnret kdnnqgnssq akkkniivqv pqaaqivlll
      841 gkqkekslqq sktnvsvsqs k
//
LOCUS       XP_034195871             861 aa            linear   INV 03-SEP-2025
DEFINITION  daxx-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034195871
VERSION     XP_034195871.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195871.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="daxx-like protein"
                     /calculated_mol_wt=98347
     Region          273..484
                     /region_name="DAXX_histone_binding"
                     /note="Histone binding domain of the death-domain
                     associated protein (DAXX); cd13150"
                     /db_xref="CDD:240523"
     Site            order(284..285,288,291..292,294..300,302..305,307..310,
                     312..313,316,319..320,323,336..338,384..388,391,418,
                     422..423,426,429,432..433,436..437,439..441,443,468,
                     471..472,475..476,478..479,482..483)
                     /site_type="other"
                     /note="Histone H3.3 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     Site            order(298,384,386,389,430,433..434,437,442,447..450,453,
                     456,460,463,467,470..471,474..475,478,482)
                     /site_type="other"
                     /note="Histone H4 interface [polypeptide binding]"
                     /db_xref="CDD:240523"
     CDS             1..861
                     /gene="Daxx"
                     /coded_by="XM_034339980.2:643..3228"
                     /db_xref="GeneID:117611760"
ORIGIN      
        1 meseiiciss dddesmehnk tnntkssvik qtkdehsssi kerstlsrne itkinkkkrk
       61 isevidenda cfknqakvim ctedsitkrd aiknsdelpt vqkdntlssd edvmllkpkl
      121 vvkekksifl eeqdifpmfi slclqkdrse dmktvvnklk rryehldpty atsetfinll
      181 nekrndimls knklyihiae vmnemknnck knsalltnga ncvtninkln tgnssaktlr
      241 sstshntvsd neemlttgek ndkdekrdgk hnsqrkirvi lkamrkceky iklleesevd
      301 fdqennsnyi klerykhriv elhqiyceci gesadagrqy lrpkhfsttg ivsvdhaits
      361 finskmskrs klkkigaftn alifpdyrdi lecvtkcnem nnlgldnkkq qqiakkafte
      421 lgeylqrsrr ndywdtfslf fenkddddpa lkdrqlaekl mqnkqegekr lanvfqeyvk
      481 kqeekndhsi dsetssenee needctened mdinnetdni sedgtslnnd dnkastdede
      541 ngidgskqsv skmdinnkps vnevqynvie irekqqtksn nklkdnvsnn nlkikqvdid
      601 enisksehtq likenetiql sdtvphnlnt spgliplttl smsdnkipie kakeddsnii
      661 neklinepps eeekpllrvr sfaklpgtwk diqekvgqld knndlidltq espkqnstah
      721 qqasiqsksk vlpttkdkrk mlvipsgkhi ikvqnvtnny mrigskssmd fnnkaklqke
      781 vvtssyqivg nssrtttirl ppnqnfnret kdnnqgnssq akkkniivqv pqaaqivlll
      841 gkqkekslqq sktnvsvsqs k
//
LOCUS       XP_034194436             507 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610773 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194436
VERSION     XP_034194436.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338545.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="uncharacterized protein LOC117610773 isoform X1"
                     /calculated_mol_wt=58290
     Region          55..>225
                     /region_name="SelP_N"
                     /note="Selenoprotein P, N terminal region; pfam04592"
                     /db_xref="CDD:461363"
     CDS             1..507
                     /gene="LOC117610773"
                     /coded_by="XM_034338545.2:396..1919"
                     /db_xref="GeneID:117610773"
ORIGIN      
        1 msifngilii lsvagcigqr sprtpetpsr hfrqfeesre nrgyveqcra tnlwkgqlah
       61 vnviaffdps wyysyrqtim lkllkrrldk sgftnilffc vappsglpeg ntensveikt
      121 wreisknvwk seylfdidqr vlddstksky diifiqdnlk lriwenfral kdevviidrc
      181 grltyqvivp wsilyfpyvk aailstykdd pcgpcnvqss vdrdsmdyee yllknmnlng
      241 nldpdkkkid nkqststitp fstnpstist tdieigttse vkeeqeqiqi sstisnsgmt
      301 esytgtgdhi llnnqtnidn nsstnedaqn eepkalplri ilyaphlhqe gkmlkkythl
      361 vlktgspdyh dhfnvkntvs deenkmlvsq lnttledkkl teyvdsines pglygeisdy
      421 wrtqnneevn dknentefld yddttvdgse snindafvse ssivrpseid tintdpnien
      481 nddandftqq rlmehysrll twiyyii
//
LOCUS       XP_034194447             505 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610773 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194447
VERSION     XP_034194447.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338556.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="uncharacterized protein LOC117610773 isoform X2"
                     /calculated_mol_wt=58048
     Region          55..>225
                     /region_name="SelP_N"
                     /note="Selenoprotein P, N terminal region; pfam04592"
                     /db_xref="CDD:461363"
     CDS             1..505
                     /gene="LOC117610773"
                     /coded_by="XM_034338556.2:407..1924"
                     /db_xref="GeneID:117610773"
ORIGIN      
        1 msifngilii lsvagcigqr sprtpetpsr hfrqfeesre nrgyveqcra tnlwkgqlah
       61 vnviaffdps wyysyrqtim lkllkrrldk sgftnilffc vappsglpeg ntensveikt
      121 wreisknvwk seylfdidqr vlddstksky diifiqdnlk lriwenfral kdevviidrc
      181 grltyqvivp wsilyfpyvk aailstykdd pcgpcnvqss vdrdsmdyee yllknmnlng
      241 nldpdkkkid nkqststitp fstnpstist tdieigttse vkeeqeqiqi sstisnsgmt
      301 esytgtgdhi llnnqtnidn nsstnedaqn eepkalplri ilyaphlhqe gkmlkkythl
      361 vlktgspdyh dhfnvkntvs deemlvsqln ttledkklte yvdsinespg lygeisdywr
      421 tqnneevndk nentefldyd dttvdgsesn indafvsess ivrpseidti ntdpniennd
      481 dandftqqrl mehysrlltw iyyii
//
LOCUS       XP_034194456             462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610773 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194456
VERSION     XP_034194456.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338565.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="uncharacterized protein LOC117610773 isoform X3"
                     /calculated_mol_wt=53056
     Region          55..>225
                     /region_name="SelP_N"
                     /note="Selenoprotein P, N terminal region; pfam04592"
                     /db_xref="CDD:461363"
     CDS             1..462
                     /gene="LOC117610773"
                     /coded_by="XM_034338565.2:403..1791"
                     /db_xref="GeneID:117610773"
ORIGIN      
        1 msifngilii lsvagcigqr sprtpetpsr hfrqfeesre nrgyveqcra tnlwkgqlah
       61 vnviaffdps wyysyrqtim lkllkrrldk sgftnilffc vappsglpeg ntensveikt
      121 wreisknvwk seylfdidqr vlddstksky diifiqdnlk lriwenfral kdevviidrc
      181 grltyqvivp wsilyfpyvk aailstykdd pcgpcnvqss vdrdsmdyee yllknmnlng
      241 nldpdkkkid nkqststitp fstnpstist tdieigttse vkeeqeqiqi sstisnsgmt
      301 esytgtgdhi llnnqtnidn nsstnedaqn eepkalplri ilyaphlhqe gkmlkkythl
      361 vlktgspdyh dhfnvkntvs deenkmlvsq lnttledkkl teyvdsines pglygeisdy
      421 wrtqnneevn dknentefld yddttmvqrv ilmmlslvkv vs
//
LOCUS       XP_034193323             451 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610256 [Osmia lignaria lignaria].
ACCESSION   XP_034193323
VERSION     XP_034193323.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337432.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193323.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..451
                     /product="uncharacterized protein LOC117610256"
                     /calculated_mol_wt=52638
     Region          238..451
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..451
                     /gene="LOC117610256"
                     /coded_by="XM_034337432.2:250..1605"
                     /db_xref="GeneID:117610256"
ORIGIN      
        1 makkikknan svkinntdis ektvdkkkkq qqkkldknkv tiirdqnlqf ksklkknkyq
       61 lakklkqiqg eeksrtftdi vdqksvkiln pknrgegpks kfqivnkkqr rkgknserhk
      121 hkeqkkemkq kkqkkemkqn ehkikiknsn qsksktnsdk qflnekqcks tmhkknkkqi
      181 gdnktkvdsa lhkikkkkst pkkhqldikk lkklvatkqe eikdqkentk prslrermmt
      241 klkasrfryl neslyssesl esknyfkndp dafkayhegy kqqvdqwpln pldiiitsik
      301 kmpkthviad fgcgegklav svphkvhsfd fislnenvta cdvahtpllt sgvdvvvfcl
      361 slmgtnlkdy iieanrvlkk ngilkiaeve srfeqvedfi kilesygfkn twkdlshnlf
      421 yfldfrkekd ignkrnklpp ivlkpclykk r
//
LOCUS       XP_034193665             391 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit M [Osmia
            lignaria lignaria].
ACCESSION   XP_034193665
VERSION     XP_034193665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337774.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..391
                     /product="eukaryotic translation initiation factor 3
                     subunit M"
                     /calculated_mol_wt=44314
     Region          239..338
                     /region_name="PCI"
                     /note="PCI domain; pfam01399"
                     /db_xref="CDD:460195"
     Region          343..371
                     /region_name="eIF3m_C_helix"
                     /note="eIF3 subunit M, C-terminal helix; pfam18005"
                     /db_xref="CDD:465614"
     CDS             1..391
                     /gene="eIF3m"
                     /coded_by="XM_034337774.2:115..1290"
                     /db_xref="GeneID:117610416"
ORIGIN      
        1 mqvppifmel pledqaqelr vyfkslgaei seekspkgie ddlhkiigvc eacfkegnen
       61 eietvlndiv simivipter aenlilafce kltkapgykl glvclkalwl lfqslsddsp
      121 mryhvyyhlv qiarnvdqvk avysgidqlk qqfasfppsn eqmqkllrll hevllsckqg
      181 eqaaavmvel lgtytaenas aaredaqrci laaladpntf lldpllalkp vrflegelih
      241 dlllvfvqdk lpaylhfyqh hkefvehqlg lnheqnmkkm rlltfmqlae tnpemsfdti
      301 qeelqinede vesfiidvlk tklvrarmdq agrkvlisst mhrtfgrpqw mqlrdllaaw
      361 kanlsavqeg mksvaaaqme laaknkaain h
//
LOCUS       XP_034193678             407 aa            linear   INV 03-SEP-2025
DEFINITION  proline-rich Akt substrate 40 kDa [Osmia lignaria lignaria].
ACCESSION   XP_034193678
VERSION     XP_034193678.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337787.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..407
                     /product="proline-rich Akt substrate 40 kDa"
                     /calculated_mol_wt=45518
     Region          312..405
                     /region_name="PRAS"
                     /note="Proline-rich AKT1 substrate 1; pfam15798"
                     /db_xref="CDD:406279"
     CDS             1..407
                     /gene="PRAS40"
                     /coded_by="XM_034337787.2:751..1974"
                     /db_xref="GeneID:117610422"
ORIGIN      
        1 mhiackclnv siksrgtelq kvnindielt peeqndgffh qnlstisele gitkeqpglv
       61 eirnvgswvi hrcyncsmyt havhrdygaa lvlintdivt speeinklrs npdyspvfri
      121 iiahntldel dylqqptkfs vsqlpnniqm algglqqqle eavqqqsaei enkiraftte
      181 qyqlleqfre rahnehrllt rlickgeetn rltnnietpp ttpenfttnl ttsttntvnt
      241 sqvisnetkv itgpnikqks gakyflstlv ngnidkkdkl yictkdptsf dtealfpleg
      301 vedaipndqv qsseegsdtd dsgqdegihm prgqrgghpt lakslpvsvp sfpsfvrrtv
      361 qdqdddqlsr dphdphnira sikalaksvh gdtvfgdlpr prfstqi
//
LOCUS       XP_034193692             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193692
VERSION     XP_034193692.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337801.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_034337801.2:378..1508"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_034193701             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193701
VERSION     XP_034193701.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337810.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_034337810.2:375..1505"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_034193721             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193721
VERSION     XP_034193721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337830.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_034337830.2:152..1282"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_034193730             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193730
VERSION     XP_034193730.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337839.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_034337839.2:143..1273"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_034193737             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193737
VERSION     XP_034193737.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337846.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_034337846.2:38..1168"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_076546609             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546609
VERSION     XP_076546609.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690494.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_076690494.1:56..1186"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_034193756             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193756
VERSION     XP_034193756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_034337865.2:112..1242"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_034193747             376 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193747
VERSION     XP_034193747.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337856.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="ethanolamine kinase 1 isoform X1"
                     /calculated_mol_wt=44542
     Region          72..367
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(73,75,77..78,80,94,96,125,137..140,142..144,240,242,
                     244..245,247,258..259,262,278,283)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(73,78,80,94,96,125,137..140,142..144,244,247,
                     258..259)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(75,77,240,242,245,262,278,283)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(208..211,215..216,222,226,330,354..355,358,361..362,
                     365..367)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..376
                     /gene="eas"
                     /coded_by="XM_034337856.2:35..1165"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkfvmiigtl lcyqifttll
       61 mvvcfscfdy iftdgitnkl igvwysghyn dmvlvrvygh ktdllinrkd etrnirilnk
      121 agfthslyat fnnglayqfi egntlttetv rnpdiytlia krmaqmhkln pdndeipkqa
      181 ciwnklekfm eimpkefcdd tkqsrlekli kpfsvlkqny qllkeeltks nskvvfahnd
      241 lllgnvlynq kensitfidf eytaynyqaf dianhfaefa gidnpdfsly peeqlqkrwl
      301 yiylqeynnv nyvpenelnl lyvqvnkfvl lshffwgcwg liqsehstid fdfleyaair
      361 fneyfkwkee ylnmel
//
LOCUS       XP_034193765             348 aa            linear   INV 03-SEP-2025
DEFINITION  ethanolamine kinase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193765
VERSION     XP_034193765.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337874.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..348
                     /product="ethanolamine kinase 1 isoform X2"
                     /calculated_mol_wt=41275
     Region          39..339
                     /region_name="ETNK_euk"
                     /note="Euykaryotic Ethanolamine kinase; cd05157"
                     /db_xref="CDD:270706"
     Site            order(45,47,49..50,52,66,68,97,109..112,114..116,212,214,
                     216..217,219,230..231,234,250,255)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:270706"
     Site            order(45,50,52,66,68,97,109..112,114..116,216,219,
                     230..231)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(47,49,212,214,217,234,250,255)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:270706"
     Site            order(180..183,187..188,194,198,302,326..327,330,333..334,
                     337..339)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270706"
     CDS             1..348
                     /gene="eas"
                     /coded_by="XM_034337874.2:378..1424"
                     /db_xref="GeneID:117610428"
ORIGIN      
        1 mdqickephl nitideneiv dgakeiikri rpmwplnnlh fkiftdgitn kligvwysgh
       61 yndmvlvrvy ghktdllinr kdetrniril nkagfthsly atfnnglayq fiegntltte
      121 tvrnpdiytl iakrmaqmhk lnpdndeipk qaciwnklek fmeimpkefc ddtkqsrlek
      181 likpfsvlkq nyqllkeelt ksnskvvfah ndlllgnvly nqkensitfi dfeytaynyq
      241 afdianhfae fagidnpdfs lypeeqlqkr wlyiylqeyn nvnyvpenel nllyvqvnkf
      301 vllshffwgc wgliqsehst idfdfleyaa irfneyfkwk eeylnmel
//
LOCUS       XP_034195875             358 aa            linear   INV 03-SEP-2025
DEFINITION  innexin 2 [Osmia lignaria lignaria].
ACCESSION   XP_034195875
VERSION     XP_034195875.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339984.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="innexin 2"
                     /calculated_mol_wt=41403
     Region          1..349
                     /region_name="Innexin"
                     /note="cl03000"
                     /db_xref="CDD:470716"
     CDS             1..358
                     /gene="Inx2"
                     /coded_by="XM_034339984.2:593..1669"
                     /db_xref="GeneID:117611763"
ORIGIN      
        1 mfdvfgsvkg llkldsvcid nnvfrlhyka tviiliafsl lvtsrqyigd pidcivdeip
       61 lhvmdtycwi ystftipdrt givgkdivqp gvashvegdd eikyhkyyqw vcftlffqai
      121 lfyvprylwk tweggrikml vldlncpvms edckterrkl lvdyftsnrh tqnfyafrff
      181 lcevlnfvnv vgqiyfmdff ldgefttygs dvvkftemep eeridpmsrv fpkvtkctfh
      241 kygasgtvqk fdglcvlpln ivnekiyvfl wfwfiilsvl sgltlayraa vvagpklrli
      301 llrarsrlsp qedieliakd cqigdwfvly qlgknidplv yqqliselal klqgkepv
//
LOCUS       XP_076546610             312 aa            linear   INV 03-SEP-2025
DEFINITION  kynurenine formamidase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546610
VERSION     XP_076546610.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690495.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="kynurenine formamidase isoform X1"
                     /calculated_mol_wt=35127
     Region          78..>206
                     /region_name="Aes"
                     /note="Acetyl esterase/lipase [Lipid transport and
                     metabolism]; COG0657"
                     /db_xref="CDD:440422"
     CDS             1..312
                     /gene="KFase"
                     /coded_by="XM_076690495.1:39..977"
                     /db_xref="GeneID:117611764"
ORIGIN      
        1 mrssttitqw llenkirver cyeqevnytp skwskrlgha evlahyykfa kevtenarkt
       61 lkceldvayg ttdrtkydiy gidlpkdapi fvfihggywq egskdmaafa apvlickgik
      121 vitvgydlcp nvklrdiisq ikkaieqilk yaltlgsrgv wisghsagah lassllfdpl
      181 wmekitksgy lnllkgvili ggvfnlkpli nttindnlkl tkdeiveysf ttldtknnkp
      241 iqgvkvivtv aecdspglin esrecaqkli tivdnvqyif lrdnidhfdi vekftdeefv
      301 ltkiilntvk vg
//
LOCUS       XP_034195876             294 aa            linear   INV 03-SEP-2025
DEFINITION  kynurenine formamidase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195876
VERSION     XP_034195876.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339985.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="kynurenine formamidase isoform X2"
                     /calculated_mol_wt=32835
     Region          60..>188
                     /region_name="Aes"
                     /note="Acetyl esterase/lipase [Lipid transport and
                     metabolism]; COG0657"
                     /db_xref="CDD:440422"
     CDS             1..294
                     /gene="KFase"
                     /coded_by="XM_034339985.2:106..990"
                     /db_xref="GeneID:117611764"
ORIGIN      
        1 msiseqevny tpskwskrlg haevlahyyk fakevtenar ktlkceldva ygttdrtkyd
       61 iygidlpkda pifvfihggy wqegskdmaa faapvlickg ikvitvgydl cpnvklrdii
      121 sqikkaieqi lkyaltlgsr gvwisghsag ahlassllfd plwmekitks gylnllkgvi
      181 liggvfnlkp linttindnl kltkdeivey sfttldtknn kpiqgvkviv tvaecdspgl
      241 inesrecaqk litivdnvqy iflrdnidhf divekftdee fvltkiilnt vkvg
//
LOCUS       XP_034193778             292 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610459 [Osmia lignaria lignaria].
ACCESSION   XP_034193778
VERSION     XP_034193778.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337887.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="uncharacterized protein LOC117610459"
                     /calculated_mol_wt=33392
     CDS             1..292
                     /gene="LOC117610459"
                     /coded_by="XM_034337887.2:151..1029"
                     /db_xref="GeneID:117610459"
ORIGIN      
        1 mnnteelmsd iyntlvkiry pkitttvaqn vysmilsges risllswilt ekspeiatkl
       61 qklkgdilea elfkcyseig ictdknlllg nckledelst lrllldfikc mfiessdiey
      121 eekesiddil nvyinedlsa ltsivkpkls ysealeyfdn lqkqldesss cklekehlee
      181 hpteikreen neeeqtsifn tekenfieaf snigswpmlk tkstrsnlys mdadieniys
      241 nflsltqflq akeeicnasi pkeiskints lseiveetav ateeilnvtn ny
//
LOCUS       XP_034194468             175 aa            linear   INV 03-SEP-2025
DEFINITION  mesencephalic astrocyte-derived neurotrophic factor [Osmia lignaria
            lignaria].
ACCESSION   XP_034194468
VERSION     XP_034194468.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338577.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="mesencephalic astrocyte-derived neurotrophic
                     factor"
                     /calculated_mol_wt=19829
     Region          26..121
                     /region_name="ARMET_N"
                     /note="ARMET, N-terminal; pfam20145"
                     /db_xref="CDD:466306"
     Region          125..167
                     /region_name="ARMET_C"
                     /note="ARMET, C-terminal; pfam10208"
                     /db_xref="CDD:462997"
     CDS             1..175
                     /gene="Manf"
                     /coded_by="XM_034338577.2:206..733"
                     /db_xref="GeneID:117610780"
ORIGIN      
        1 mnksmliifs iiigiisvtt alkndecevc istvekfvnt lsddvkkntk kieaafkefc
       61 kgtkskenrf cyylgglees atgilgelsk piswsmpank vceklkkmda qicdlrfekq
      121 idvntvdlkk lkvrdlkkil sewdetcegc iektdfikri eelkpkyshs sksel
//
LOCUS       XP_034193337             151 aa            linear   INV 03-SEP-2025
DEFINITION  succinate dehydrogenase assembly factor 2, mitochondrial [Osmia
            lignaria lignaria].
ACCESSION   XP_034193337
VERSION     XP_034193337.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337446.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="succinate dehydrogenase assembly factor 2,
                     mitochondrial"
                     /calculated_mol_wt=17630
     Region          61..136
                     /region_name="Sdh5"
                     /note="Flavinator of succinate dehydrogenase; pfam03937"
                     /db_xref="CDD:461097"
     CDS             1..151
                     /gene="LOC117610259"
                     /coded_by="XM_034337446.2:214..669"
                     /db_xref="GeneID:117610259"
ORIGIN      
        1 mntlaksfvl rvnpvhsvrn vsasctrhkd dfinlihpeg qeptipqyve rneegidvkr
       61 arlvyqsrkr gmlengllls tfvkkylhef ddkqlqlydh linlptndwd ifywatgakp
      121 tpaefdnevm dllkkhirne drqarimqpe l
//
LOCUS       XP_034193792             143 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S23 [Osmia lignaria lignaria].
ACCESSION   XP_034193792
VERSION     XP_034193792.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337901.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="ribosomal protein S23"
                     /calculated_mol_wt=15896
     Region          1..143
                     /region_name="PTZ00067"
                     /note="40S ribosomal S23; Provisional"
                     /db_xref="CDD:185422"
     Site            order(26,28..32,34,36..41,43..49,60..68,74..76,84,86..89,
                     102..108,111..114,120..121,126,128,138..140)
                     /site_type="other"
                     /note="18S rRNA interaction site [nucleotide binding]"
                     /db_xref="CDD:239465"
     Site            order(60..61,112)
                     /site_type="other"
                     /note="streptomycin interaction site [chemical binding]"
                     /db_xref="CDD:239465"
     Site            61..62
                     /site_type="other"
                     /note="28S rRNA interaction site [nucleotide binding]"
                     /db_xref="CDD:239465"
     Site            order(62..67,88..96)
                     /site_type="other"
                     /note="aminoacyl-tRNA interaction site (A-site)
                     [nucleotide binding]"
                     /db_xref="CDD:239465"
     Site            97
                     /site_type="other"
                     /note="eEF2 interaction site [polypeptide binding]"
                     /db_xref="CDD:239465"
     CDS             1..143
                     /gene="RpS23"
                     /coded_by="XM_034337901.1:136..567"
                     /db_xref="GeneID:117610463"
ORIGIN      
        1 mgkprglrta rkhvnhrrdq rwndkdykka hlgtrwkanp fggashakgi vlekvgveak
       61 qpnsairkcv rvqlikngkk itafvprdgc lnnieendev lvagfgrkgh avgdipgvrf
      121 kvvkvanvsl lalykekker prs
//
LOCUS       XP_034194476             131 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610787 [Osmia lignaria lignaria].
ACCESSION   XP_034194476
VERSION     XP_034194476.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338585.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194476.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..131
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..131
                     /product="uncharacterized protein LOC117610787"
                     /calculated_mol_wt=15068
     Region          19..102
                     /region_name="LLC1"
                     /note="Normal lung function maintenance, Low in Lung
                     Cancer 1 protein; pfam14945"
                     /db_xref="CDD:464392"
     CDS             1..131
                     /gene="LOC117610787"
                     /coded_by="XM_034338585.2:70..465"
                     /db_xref="GeneID:117610787"
ORIGIN      
        1 mdardfkard fvsvsknfte rmknerwsqi kwlqryewll eeqaklnkel eelwkekqtv
       61 lptegfvreg gktclpapvt tsgeygwlas kpkfqlekyg syipqypdpl kdvvlltgni
      121 pllaagkgfi l
//
LOCUS       XP_034195422             132 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611566 [Osmia lignaria lignaria].
ACCESSION   XP_034195422
VERSION     XP_034195422.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339531.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..132
                     /product="uncharacterized protein LOC117611566"
                     /calculated_mol_wt=14620
     Region          38..77
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..132
                     /gene="LOC117611566"
                     /coded_by="XM_034339531.2:146..544"
                     /db_xref="GeneID:117611566"
ORIGIN      
        1 mkglffitia vylayvvygq aptvncppyd qnsdgpllvp hpcncsayyv cfspvnipmp
       61 cpvglqfnna tkkcdwpwiv kcrpqincpg akiqdavesh eelsreeevv iqeeqgevsr
      121 eqvgklsaaf fh
//
LOCUS       XP_034195423             111 aa            linear   INV 03-SEP-2025
DEFINITION  peritrophin-1-like [Osmia lignaria lignaria].
ACCESSION   XP_034195423
VERSION     XP_034195423.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195423.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..111
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..111
                     /product="peritrophin-1-like"
                     /calculated_mol_wt=11895
     Region          37..81
                     /region_name="CBM_14"
                     /note="Chitin binding Peritrophin-A domain; pfam01607"
                     /db_xref="CDD:426342"
     CDS             1..111
                     /gene="LOC117611567"
                     /coded_by="XM_034339532.2:116..451"
                     /db_xref="GeneID:117611567"
ORIGIN      
        1 mkgvfeillt vvaflllvvs lanavvcppk neadvtllpn pdncqtfylc ndgipylmqc
       61 pdgldfnpke rvcdlpeqan cissvvtttt stpsgydadf eassededfg l
//
LOCUS       XP_076546611             314 aa            linear   INV 03-SEP-2025
DEFINITION  CD151 antigen [Osmia lignaria lignaria].
ACCESSION   XP_076546611
VERSION     XP_076546611.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690496.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..314
                     /product="CD151 antigen"
                     /calculated_mol_wt=34263
     Region          9..>160
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          116..240
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cd03127"
                     /db_xref="CDD:239401"
     Site            order(117,122,126,128..129,132,148,151..152,155..156)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239401"
     CDS             1..314
                     /gene="LOC117611612"
                     /coded_by="XM_076690496.1:277..1221"
                     /db_xref="GeneID:117611612"
ORIGIN      
        1 mdrkslgvsr ifvccsnvif lisgfalmsl gallladder illsrllgpg dihpnqplfy
       61 ylafaivglg flitltgllg cwatciynrc vtisylitit lllfgectvc vlvvfwphvl
      121 gidvrsarli ralqrsyavp greqftaald laqttfaccg ingssnygts wwrlqevgrr
      181 elvvplscct lnnanqtdsf lnpepanltl cqalnpaehq yarhttgcle mlekwtqdqa
      241 lilltvglav vfvevgalls tffacsrgnr raksqastft stqtlspfse sdhdfglgie
      301 nrmhvagttf gaks
//
LOCUS       XP_034195523             723 aa            linear   INV 03-SEP-2025
DEFINITION  cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195523
VERSION     XP_034195523.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339632.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..723
                     /product="cGMP-dependent 3',5'-cyclic phosphodiesterase
                     isoform X1"
                     /calculated_mol_wt=83185
     Region          195..345
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          439..671
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..723
                     /gene="LOC117611621"
                     /coded_by="XM_034339632.2:276..2447"
                     /db_xref="GeneID:117611621"
ORIGIN      
        1 mnaislntsn aenilsllee lcdlsypqvq qklnkyiqnv tkakvsflvp ilikseemvi
       61 hvigekildr elrfpvsnni lyktlqnkkp ttaditmlde ellrhlqpic pmsksfltip
      121 iqhpkqnhva lvvslvddnn qneitqecae vvqecfrfcl gfllnslicy eetrlkqqcq
      181 kllavsrklf thlgdfsdll reimaevrnl tnaercslfl ldpdqqdlva kvfdgiamke
      241 sikemripig qgiaghvatt gkllnirnay ehplfysgid evtgfrtrni lcfpirdeng
      301 ivgvaqlcnk kdglyfdvfd eevatafsiy cgisimhsiv ykkmqdaqar nklsnevmmy
      361 hmkveedavq allnckdehn ikdfdkfqft prsvpykhmp cytikmfndl glikhwkmkl
      421 stlarfilyv kkgyrdapyh nwvhafsvah faylliknle litenymtql qalvflvscl
      481 chdidhrgtn nsfqtkcstv laslyssegs vmerhhlaqt mcilntencn ifenfnsdey
      541 sealdllrnn ilatdlashf rtvekqeemi knnfdkkdsk hlklffsmlm tccdlsdqtk
      601 hwkvskktae qiydeffsqg dleksmgssp iemmdreras ipdlqvqfit nivlplfinl
      661 stlfpviqpl vhvlnenrvl weesrsifqk yvktgmkgid illdptfeee vlqisiqsnn
      721 dll
//
LOCUS       XP_034195524             666 aa            linear   INV 03-SEP-2025
DEFINITION  cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195524
VERSION     XP_034195524.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339633.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..666
                     /product="cGMP-dependent 3',5'-cyclic phosphodiesterase
                     isoform X2"
                     /calculated_mol_wt=76765
     Region          138..288
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          382..614
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..666
                     /gene="LOC117611621"
                     /coded_by="XM_034339633.2:130..2130"
                     /db_xref="GeneID:117611621"
ORIGIN      
        1 mvihvigeki ldrelrfpvs nnilyktlqn kkpttaditm ldeellrhlq picpmsksfl
       61 tipiqhpkqn hvalvvslvd dnnqneitqe caevvqecfr fclgfllnsl icyeetrlkq
      121 qcqkllavsr klfthlgdfs dllreimaev rnltnaercs lflldpdqqd lvakvfdgia
      181 mkesikemri pigqgiaghv attgkllnir nayehplfys gidevtgfrt rnilcfpird
      241 engivgvaql cnkkdglyfd vfdeevataf siycgisimh sivykkmqda qarnklsnev
      301 mmyhmkveed avqallnckd ehnikdfdkf qftprsvpyk hmpcytikmf ndlglikhwk
      361 mklstlarfi lyvkkgyrda pyhnwvhafs vahfayllik nlelitenym tqlqalvflv
      421 sclchdidhr gtnnsfqtkc stvlaslyss egsvmerhhl aqtmcilnte ncnifenfns
      481 deysealdll rnnilatdla shfrtvekqe emiknnfdkk dskhlklffs mlmtccdlsd
      541 qtkhwkvskk taeqiydeff sqgdleksmg sspiemmdre rasipdlqvq fitnivlplf
      601 inlstlfpvi qplvhvlnen rvlweesrsi fqkyvktgmk gidilldptf eeevlqisiq
      661 snndll
//
LOCUS       XP_034195525             654 aa            linear   INV 03-SEP-2025
DEFINITION  cGMP-dependent 3',5'-cyclic phosphodiesterase isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195525
VERSION     XP_034195525.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339634.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..654
                     /product="cGMP-dependent 3',5'-cyclic phosphodiesterase
                     isoform X3"
                     /calculated_mol_wt=75379
     Region          126..276
                     /region_name="GAF"
                     /note="Domain present in phytochromes and cGMP-specific
                     phosphodiesterases; smart00065"
                     /db_xref="CDD:214500"
     Region          370..602
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..654
                     /gene="LOC117611621"
                     /coded_by="XM_034339634.2:148..2112"
                     /db_xref="GeneID:117611621"
ORIGIN      
        1 mkpyievsnn ilyktlqnkk pttaditmld eellrhlqpi cpmsksflti piqhpkqnhv
       61 alvvslvddn nqneitqeca evvqecfrfc lgfllnslic yeetrlkqqc qkllavsrkl
      121 fthlgdfsdl lreimaevrn ltnaercslf lldpdqqdlv akvfdgiamk esikemripi
      181 gqgiaghvat tgkllnirna yehplfysgi devtgfrtrn ilcfpirden givgvaqlcn
      241 kkdglyfdvf deevatafsi ycgisimhsi vykkmqdaqa rnklsnevmm yhmkveedav
      301 qallnckdeh nikdfdkfqf tprsvpykhm pcytikmfnd lglikhwkmk lstlarfily
      361 vkkgyrdapy hnwvhafsva hfaylliknl elitenymtq lqalvflvsc lchdidhrgt
      421 nnsfqtkcst vlaslysseg svmerhhlaq tmcilntenc nifenfnsde ysealdllrn
      481 nilatdlash frtvekqeem iknnfdkkds khlklffsml mtccdlsdqt khwkvskkta
      541 eqiydeffsq gdleksmgss piemmdrera sipdlqvqfi tnivlplfin lstlfpviqp
      601 lvhvlnenrv lweesrsifq kyvktgmkgi dilldptfee evlqisiqsn ndll
//
LOCUS       XP_034196015            1457 aa            linear   INV 03-SEP-2025
DEFINITION  SNF4/AMP-activated protein kinase gamma subunit isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196015
VERSION     XP_034196015.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340124.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196015.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1457
                     /product="SNF4/AMP-activated protein kinase gamma subunit
                     isoform X1"
                     /calculated_mol_wt=161859
     Region          1073..1209
                     /region_name="CBS_euAMPK_gamma-like_repeat1"
                     /note="Two tandem repeats of the cystathionine
                     beta-synthase (CBS pair) domains found in AMP-activated
                     protein kinase gamma-like proteins, repeat 1; cd04618"
                     /db_xref="CDD:341388"
     Site            order(1081,1083..1085,1107..1109,1187,1201,1203,
                     1205..1206,1209)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1081..1154
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Site            order(1107,1121,1125..1126,1129,1165,1187..1189,1205)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1165..1209
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Region          1235..1358
                     /region_name="CBS_euAMPK_gamma-like_repeat2"
                     /note="CBS pair domain found in 5'-AMP (adenosine
                     monophosphate)-activated protein kinase; cd04641"
                     /db_xref="CDD:341399"
     Site            order(1236,1238..1240,1262..1264,1334,1347,1349,
                     1351..1352,1355)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1236..1301
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     Site            order(1262,1275,1279..1280,1283,1312,1334..1336,1351)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1312..1355
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     CDS             1..1457
                     /gene="SNF4Agamma"
                     /coded_by="XM_034340124.2:1505..5878"
                     /db_xref="GeneID:117611811"
ORIGIN      
        1 messggdkqn esddcdvtan nqhqnkmtgq edtdvkikkn lgkqeilknl knlenigqnd
       61 eatnklpktv sqddeeeekd dddddddddd ddddddddde eeeeeeeeec ckgsdedelk
      121 eektdgkkde essssttsts asddesetss edkteaskte qiktvtteqe etaemnkeke
      181 kekekkkedq ttknddttdd sdieddlisa inyntitsla alkdmlqssg edsdgvdsff
      241 hhyflshvnr lpsrnqthsp ypwgrrlsec reedeyetee gknveslana netkkdtskt
      301 eeseekndsl sskasspsvs eksssekidq ksnlpsliss eskptatsav tttttsmitt
      361 ttttttptpt ptmtttttmt tttatttttt tatttlttpt tsttttsrft tctvtsptst
      421 tkpplrrrht tgpgmtfpat dppsystsmt fsrtsplpsp hldkrffdss liemksqass
      481 sstldydste evwirrvdfv qerkrkelgs qplptivted adnsnhasqd qghrpragtw
      541 gshsrsirkp tsgsapssgk stplpqpave pssssssskg grkasgtrsg stsrsnsrsn
      601 saerrrsgst vddtasptkk palfdafrpr sksdaskrkp siianmksav qhslhrgshg
      661 ssstdvhtdk dhhrdskdhk egreqpvgrp ragsessrnp vskvmdlirh rshsalssdd
      721 krkartaaqh qaqiaaqapl dtcesrdayd lknrtdslnv pqkrgnssss kmhginhlls
      781 hhgshngdar tgvaqpnqee kslsasdttr qqntkilqel lsqkhiqkri srgsvsstys
      841 ygsstsygss hgssssnhss slyhgannhs vyhtihgnsh ythqtnhhhh hplqhihqmh
      901 qtnsgraspt spsptgdqek hhhqhhhhys iqemirhfgr rlghirrqse cqdpprkkee
      961 dfrnrsqsld ggarhpslrd adcettyriy esilrqgalr rssldpgarr fslgtpaiph
     1021 rasdayldpv haailfrdsr glpvvdpfle kvslsdleed esqifvkffk fhkcydlipt
     1081 saklvvfdth llvkkaffal vyngvraapl wdsgrqefvg mltitdfiki lqmyytspsv
     1141 tmdeleehel dtwrkvlkdq vhplvsispd aslyeaiktl iqnrihrlpv idpdtgnvly
     1201 ilthkrilrf lflyihelpk psftnktlre lrigtfenie tateetsiil alkkfverrv
     1261 salpiidsdg klvniyskfd vinlaaekty nnldvslrea nehrnewfeg vqsckldetl
     1321 ftvmekivra evhrlvvvdd ddkvigiisl sdllfylvlr pcgengssnk dssislraqd
     1381 siaskapssl qseasipdge aevteqdtae vnesevviet pspplspats disepqsalv
     1441 nqsqesawre vtvsgge
//
LOCUS       XP_034196016            1457 aa            linear   INV 03-SEP-2025
DEFINITION  SNF4/AMP-activated protein kinase gamma subunit isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196016
VERSION     XP_034196016.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340125.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196016.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1457
                     /product="SNF4/AMP-activated protein kinase gamma subunit
                     isoform X1"
                     /calculated_mol_wt=161859
     Region          1073..1209
                     /region_name="CBS_euAMPK_gamma-like_repeat1"
                     /note="Two tandem repeats of the cystathionine
                     beta-synthase (CBS pair) domains found in AMP-activated
                     protein kinase gamma-like proteins, repeat 1; cd04618"
                     /db_xref="CDD:341388"
     Site            order(1081,1083..1085,1107..1109,1187,1201,1203,
                     1205..1206,1209)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1081..1154
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Site            order(1107,1121,1125..1126,1129,1165,1187..1189,1205)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1165..1209
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Region          1235..1358
                     /region_name="CBS_euAMPK_gamma-like_repeat2"
                     /note="CBS pair domain found in 5'-AMP (adenosine
                     monophosphate)-activated protein kinase; cd04641"
                     /db_xref="CDD:341399"
     Site            order(1236,1238..1240,1262..1264,1334,1347,1349,
                     1351..1352,1355)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1236..1301
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     Site            order(1262,1275,1279..1280,1283,1312,1334..1336,1351)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1312..1355
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     CDS             1..1457
                     /gene="SNF4Agamma"
                     /coded_by="XM_034340125.2:1520..5893"
                     /db_xref="GeneID:117611811"
ORIGIN      
        1 messggdkqn esddcdvtan nqhqnkmtgq edtdvkikkn lgkqeilknl knlenigqnd
       61 eatnklpktv sqddeeeekd dddddddddd ddddddddde eeeeeeeeec ckgsdedelk
      121 eektdgkkde essssttsts asddesetss edkteaskte qiktvtteqe etaemnkeke
      181 kekekkkedq ttknddttdd sdieddlisa inyntitsla alkdmlqssg edsdgvdsff
      241 hhyflshvnr lpsrnqthsp ypwgrrlsec reedeyetee gknveslana netkkdtskt
      301 eeseekndsl sskasspsvs eksssekidq ksnlpsliss eskptatsav tttttsmitt
      361 ttttttptpt ptmtttttmt tttatttttt tatttlttpt tsttttsrft tctvtsptst
      421 tkpplrrrht tgpgmtfpat dppsystsmt fsrtsplpsp hldkrffdss liemksqass
      481 sstldydste evwirrvdfv qerkrkelgs qplptivted adnsnhasqd qghrpragtw
      541 gshsrsirkp tsgsapssgk stplpqpave pssssssskg grkasgtrsg stsrsnsrsn
      601 saerrrsgst vddtasptkk palfdafrpr sksdaskrkp siianmksav qhslhrgshg
      661 ssstdvhtdk dhhrdskdhk egreqpvgrp ragsessrnp vskvmdlirh rshsalssdd
      721 krkartaaqh qaqiaaqapl dtcesrdayd lknrtdslnv pqkrgnssss kmhginhlls
      781 hhgshngdar tgvaqpnqee kslsasdttr qqntkilqel lsqkhiqkri srgsvsstys
      841 ygsstsygss hgssssnhss slyhgannhs vyhtihgnsh ythqtnhhhh hplqhihqmh
      901 qtnsgraspt spsptgdqek hhhqhhhhys iqemirhfgr rlghirrqse cqdpprkkee
      961 dfrnrsqsld ggarhpslrd adcettyriy esilrqgalr rssldpgarr fslgtpaiph
     1021 rasdayldpv haailfrdsr glpvvdpfle kvslsdleed esqifvkffk fhkcydlipt
     1081 saklvvfdth llvkkaffal vyngvraapl wdsgrqefvg mltitdfiki lqmyytspsv
     1141 tmdeleehel dtwrkvlkdq vhplvsispd aslyeaiktl iqnrihrlpv idpdtgnvly
     1201 ilthkrilrf lflyihelpk psftnktlre lrigtfenie tateetsiil alkkfverrv
     1261 salpiidsdg klvniyskfd vinlaaekty nnldvslrea nehrnewfeg vqsckldetl
     1321 ftvmekivra evhrlvvvdd ddkvigiisl sdllfylvlr pcgengssnk dssislraqd
     1381 siaskapssl qseasipdge aevteqdtae vnesevviet pspplspats disepqsalv
     1441 nqsqesawre vtvsgge
//
LOCUS       XP_034196017            1457 aa            linear   INV 03-SEP-2025
DEFINITION  SNF4/AMP-activated protein kinase gamma subunit isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196017
VERSION     XP_034196017.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340126.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196017.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1457
                     /product="SNF4/AMP-activated protein kinase gamma subunit
                     isoform X1"
                     /calculated_mol_wt=161859
     Region          1073..1209
                     /region_name="CBS_euAMPK_gamma-like_repeat1"
                     /note="Two tandem repeats of the cystathionine
                     beta-synthase (CBS pair) domains found in AMP-activated
                     protein kinase gamma-like proteins, repeat 1; cd04618"
                     /db_xref="CDD:341388"
     Site            order(1081,1083..1085,1107..1109,1187,1201,1203,
                     1205..1206,1209)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1081..1154
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Site            order(1107,1121,1125..1126,1129,1165,1187..1189,1205)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1165..1209
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Region          1235..1358
                     /region_name="CBS_euAMPK_gamma-like_repeat2"
                     /note="CBS pair domain found in 5'-AMP (adenosine
                     monophosphate)-activated protein kinase; cd04641"
                     /db_xref="CDD:341399"
     Site            order(1236,1238..1240,1262..1264,1334,1347,1349,
                     1351..1352,1355)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1236..1301
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     Site            order(1262,1275,1279..1280,1283,1312,1334..1336,1351)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1312..1355
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     CDS             1..1457
                     /gene="SNF4Agamma"
                     /coded_by="XM_034340126.2:290..4663"
                     /db_xref="GeneID:117611811"
ORIGIN      
        1 messggdkqn esddcdvtan nqhqnkmtgq edtdvkikkn lgkqeilknl knlenigqnd
       61 eatnklpktv sqddeeeekd dddddddddd ddddddddde eeeeeeeeec ckgsdedelk
      121 eektdgkkde essssttsts asddesetss edkteaskte qiktvtteqe etaemnkeke
      181 kekekkkedq ttknddttdd sdieddlisa inyntitsla alkdmlqssg edsdgvdsff
      241 hhyflshvnr lpsrnqthsp ypwgrrlsec reedeyetee gknveslana netkkdtskt
      301 eeseekndsl sskasspsvs eksssekidq ksnlpsliss eskptatsav tttttsmitt
      361 ttttttptpt ptmtttttmt tttatttttt tatttlttpt tsttttsrft tctvtsptst
      421 tkpplrrrht tgpgmtfpat dppsystsmt fsrtsplpsp hldkrffdss liemksqass
      481 sstldydste evwirrvdfv qerkrkelgs qplptivted adnsnhasqd qghrpragtw
      541 gshsrsirkp tsgsapssgk stplpqpave pssssssskg grkasgtrsg stsrsnsrsn
      601 saerrrsgst vddtasptkk palfdafrpr sksdaskrkp siianmksav qhslhrgshg
      661 ssstdvhtdk dhhrdskdhk egreqpvgrp ragsessrnp vskvmdlirh rshsalssdd
      721 krkartaaqh qaqiaaqapl dtcesrdayd lknrtdslnv pqkrgnssss kmhginhlls
      781 hhgshngdar tgvaqpnqee kslsasdttr qqntkilqel lsqkhiqkri srgsvsstys
      841 ygsstsygss hgssssnhss slyhgannhs vyhtihgnsh ythqtnhhhh hplqhihqmh
      901 qtnsgraspt spsptgdqek hhhqhhhhys iqemirhfgr rlghirrqse cqdpprkkee
      961 dfrnrsqsld ggarhpslrd adcettyriy esilrqgalr rssldpgarr fslgtpaiph
     1021 rasdayldpv haailfrdsr glpvvdpfle kvslsdleed esqifvkffk fhkcydlipt
     1081 saklvvfdth llvkkaffal vyngvraapl wdsgrqefvg mltitdfiki lqmyytspsv
     1141 tmdeleehel dtwrkvlkdq vhplvsispd aslyeaiktl iqnrihrlpv idpdtgnvly
     1201 ilthkrilrf lflyihelpk psftnktlre lrigtfenie tateetsiil alkkfverrv
     1261 salpiidsdg klvniyskfd vinlaaekty nnldvslrea nehrnewfeg vqsckldetl
     1321 ftvmekivra evhrlvvvdd ddkvigiisl sdllfylvlr pcgengssnk dssislraqd
     1381 siaskapssl qseasipdge aevteqdtae vnesevviet pspplspats disepqsalv
     1441 nqsqesawre vtvsgge
//
LOCUS       XP_034196018            1453 aa            linear   INV 03-SEP-2025
DEFINITION  SNF4/AMP-activated protein kinase gamma subunit isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196018
VERSION     XP_034196018.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340127.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196018.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1453
                     /product="SNF4/AMP-activated protein kinase gamma subunit
                     isoform X2"
                     /calculated_mol_wt=161404
     Region          1073..1209
                     /region_name="CBS_euAMPK_gamma-like_repeat1"
                     /note="Two tandem repeats of the cystathionine
                     beta-synthase (CBS pair) domains found in AMP-activated
                     protein kinase gamma-like proteins, repeat 1; cd04618"
                     /db_xref="CDD:341388"
     Site            order(1081,1083..1085,1107..1109,1187,1201,1203,
                     1205..1206,1209)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1081..1154
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Site            order(1107,1121,1125..1126,1129,1165,1187..1189,1205)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341388"
     Region          1165..1209
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Region          1235..1354
                     /region_name="CBS_euAMPK_gamma-like_repeat2"
                     /note="CBS pair domain found in 5'-AMP (adenosine
                     monophosphate)-activated protein kinase; cd04641"
                     /db_xref="CDD:341399"
     Site            order(1236,1238..1240,1262..1264,1330,1343,1345,
                     1347..1348,1351)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1236..1301
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     Site            order(1262,1275,1279..1280,1283,1312,1330..1332,1347)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1312..1351
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     CDS             1..1453
                     /gene="SNF4Agamma"
                     /coded_by="XM_034340127.2:1506..5867"
                     /db_xref="GeneID:117611811"
ORIGIN      
        1 messggdkqn esddcdvtan nqhqnkmtgq edtdvkikkn lgkqeilknl knlenigqnd
       61 eatnklpktv sqddeeeekd dddddddddd ddddddddde eeeeeeeeec ckgsdedelk
      121 eektdgkkde essssttsts asddesetss edkteaskte qiktvtteqe etaemnkeke
      181 kekekkkedq ttknddttdd sdieddlisa inyntitsla alkdmlqssg edsdgvdsff
      241 hhyflshvnr lpsrnqthsp ypwgrrlsec reedeyetee gknveslana netkkdtskt
      301 eeseekndsl sskasspsvs eksssekidq ksnlpsliss eskptatsav tttttsmitt
      361 ttttttptpt ptmtttttmt tttatttttt tatttlttpt tsttttsrft tctvtsptst
      421 tkpplrrrht tgpgmtfpat dppsystsmt fsrtsplpsp hldkrffdss liemksqass
      481 sstldydste evwirrvdfv qerkrkelgs qplptivted adnsnhasqd qghrpragtw
      541 gshsrsirkp tsgsapssgk stplpqpave pssssssskg grkasgtrsg stsrsnsrsn
      601 saerrrsgst vddtasptkk palfdafrpr sksdaskrkp siianmksav qhslhrgshg
      661 ssstdvhtdk dhhrdskdhk egreqpvgrp ragsessrnp vskvmdlirh rshsalssdd
      721 krkartaaqh qaqiaaqapl dtcesrdayd lknrtdslnv pqkrgnssss kmhginhlls
      781 hhgshngdar tgvaqpnqee kslsasdttr qqntkilqel lsqkhiqkri srgsvsstys
      841 ygsstsygss hgssssnhss slyhgannhs vyhtihgnsh ythqtnhhhh hplqhihqmh
      901 qtnsgraspt spsptgdqek hhhqhhhhys iqemirhfgr rlghirrqse cqdpprkkee
      961 dfrnrsqsld ggarhpslrd adcettyriy esilrqgalr rssldpgarr fslgtpaiph
     1021 rasdayldpv haailfrdsr glpvvdpfle kvslsdleed esqifvkffk fhkcydlipt
     1081 saklvvfdth llvkkaffal vyngvraapl wdsgrqefvg mltitdfiki lqmyytspsv
     1141 tmdeleehel dtwrkvlkdq vhplvsispd aslyeaiktl iqnrihrlpv idpdtgnvly
     1201 ilthkrilrf lflyihelpk psftnktlre lrigtfenie tateetsiil alkkfverrv
     1261 salpiidsdg klvniyskfd vinlaaekty nnldvslrea nehrnewfeg vqsckldetl
     1321 ftvmekivhr lvvvddddkv igiislsdll fylvlrpcge ngssnkdssi slraqdsias
     1381 kapsslqsea sipdgeaevt eqdtaevnes evvietpspp lspatsdise pqsalvnqsq
     1441 esawrevtvs gge
//
LOCUS       XP_034196020            1198 aa            linear   INV 03-SEP-2025
DEFINITION  SNF4/AMP-activated protein kinase gamma subunit isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196020
VERSION     XP_034196020.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340129.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196020.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1198
                     /product="SNF4/AMP-activated protein kinase gamma subunit
                     isoform X3"
                     /calculated_mol_wt=132496
     Region          814..950
                     /region_name="CBS_euAMPK_gamma-like_repeat1"
                     /note="Two tandem repeats of the cystathionine
                     beta-synthase (CBS pair) domains found in AMP-activated
                     protein kinase gamma-like proteins, repeat 1; cd04618"
                     /db_xref="CDD:341388"
     Site            order(822,824..826,848..850,928,942,944,946..947,950)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341388"
     Region          822..895
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Site            order(848,862,866..867,870,906,928..930,946)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341388"
     Region          906..950
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341388"
     Region          976..1099
                     /region_name="CBS_euAMPK_gamma-like_repeat2"
                     /note="CBS pair domain found in 5'-AMP (adenosine
                     monophosphate)-activated protein kinase; cd04641"
                     /db_xref="CDD:341399"
     Site            order(977,979..981,1003..1005,1075,1088,1090,1092..1093,
                     1096)
                     /site_type="other"
                     /note="ligand binding site II [chemical binding]"
                     /db_xref="CDD:341399"
     Region          977..1042
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     Site            order(1003,1016,1020..1021,1024,1053,1075..1077,1092)
                     /site_type="other"
                     /note="ligand binding site I [chemical binding]"
                     /db_xref="CDD:341399"
     Region          1053..1096
                     /region_name="CBS repeat"
                     /note="CBS repeat [structural motif]"
                     /db_xref="CDD:341399"
     CDS             1..1198
                     /gene="SNF4Agamma"
                     /coded_by="XM_034340129.2:1505..5101"
                     /db_xref="GeneID:117611811"
ORIGIN      
        1 messggdkqn esddcdvtan nqhqnkmtgq edtdvkikkn lgkqeilknl knlenigqnd
       61 eatnklpktv sqddeeeekd dddddddddd ddddddddde eeeeeeeeec ckgsdedelk
      121 eektdgkkde essssttsts asddesetss edkteaskte qiktvtteqe etaemnkeke
      181 kekekkkedq ttknddttdd sdieddlisa inyntitsla alkdmlqssg edsdgvdsff
      241 hhyflshvnr lpsrnqthsp ypwgrrlsec reedeyetee gknveslana netkkdtskt
      301 eeseekndsl sskasspsvs eksssekidq ksnlpsliss eskptatsav tttttsmitt
      361 ttttttptpt ptmtttttmt tttatttttt tatttlttpt tsttttsrft tctvtsptst
      421 tkpplrrrht tgpgmtfpat dppsystsmt fsrtsplpsp hldkrffdss liemksqass
      481 sstldydste evwirrvdfv qerkrkelgs qplptivted adnsnhasqd qghrpragtw
      541 gshsrsirkp tsgsapssgk stplpqpave pssssssskg grkasgtrsg stsrsnsrsn
      601 saerrrsgst vddtasptkk palfdafrpr sksdaskrkp siianmksav qhslhrgshg
      661 ssstdvhtdk dhhrdskdhk egreqpvgrp ragsessrnp vskvmdlirh rshsalssdd
      721 krkartaaqh qaqiaaqgal rrssldpgar rfslgtpaip hrasdayldp vhaailfrds
      781 rglpvvdpfl ekvslsdlee desqifvkff kfhkcydlip tsaklvvfdt hllvkkaffa
      841 lvyngvraap lwdsgrqefv gmltitdfik ilqmyytsps vtmdeleehe ldtwrkvlkd
      901 qvhplvsisp daslyeaikt liqnrihrlp vidpdtgnvl yilthkrilr flflyihelp
      961 kpsftnktlr elrigtfeni etateetsii lalkkfverr vsalpiidsd gklvniyskf
     1021 dvinlaaekt ynnldvslre anehrnewfe gvqsckldet lftvmekivr aevhrlvvvd
     1081 dddkvigiis lsdllfylvl rpcgengssn kdssislraq dsiaskapss lqseasipdg
     1141 eaevteqdta evnesevvie tpspplspat sdisepqsal vnqsqesawr evtvsgge
//
LOCUS       XP_034196300             937 aa            linear   INV 03-SEP-2025
DEFINITION  WD40 repeat domain-containing protein pink isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196300
VERSION     XP_034196300.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340409.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196300.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..937
                     /product="WD40 repeat domain-containing protein pink
                     isoform X1"
                     /calculated_mol_wt=106718
     Region          28..64
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <32..157
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          71..107
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <498..>649
                     /region_name="PspF"
                     /note="Transcriptional regulators containing an AAA-type
                     ATPase domain and a DNA-binding domain [Transcription,
                     Signal transduction mechanisms]; COG1221"
                     /db_xref="CDD:440834"
     Region          892..932
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     CDS             1..937
                     /gene="pink"
                     /coded_by="XM_034340409.2:154..2967"
                     /db_xref="GeneID:117611920"
ORIGIN      
        1 mqefpcvlse yeeinpllyk pitstqriky tcfntspnyi ilgstsgsiy lflrkpctfv
       61 qliplsegsv shvlispdek tialtttrgt vclvalkptp kllaistehi qeqinclcwn
      121 dnsseiyigd gngkvsvmvm sifmmhtlqv ngmfqspact lmnldsavvq lnfcsplllv
      181 stltrcyicd tvqeqykqvg nktrngefga cfyktyltdk stlvtpkeer lvnrkgsfnf
      241 ipegnsniee gnfpqifcar pssrlwevsa ngtvmkthqf kealatppvt icrpnmrksm
      301 yqkpteqawm pqsinfshlf tiaekylfsy tstgiyiidp itatvilwsn efsnisvaet
      361 veneiylmtf dgnfhclils vldalilwly nrkryhecle vcflyktqlk qlinsteina
      421 icnienklqi lrddelstll hpliillesn sktspkklds givvvncgns nlkeeesfrh
      481 nslpyclsqn nespcetels egketltnll edstnevtki kdanyntenv qksnneerak
      541 ineelysnkt itqriqadle siyalidnik psmdeeqlek iileidwkmn vlkdsyeilt
      601 elkgfvyevl rsvelyyfna llenisvqli qpsdnetiik qimkafinin aqnykkctcg
      661 spcptdelve pkflgigryl lkkiidenke qciilcnkvp ymwreylpiy vnqhgvlmnd
      721 llrqclqikd nnllstllpl ldgkhwnlva tyakemqegq clfcgksirk gnneilinwt
      781 aviheimkkq gpdvamtlli klekaipnis vdksifqsli ftkilyhhgm khainfnknt
      841 lesseyntic stkirdqlve viekdlskpv nknifgngah hwgmhyqnkl htcpcctlsl
      901 qtpvllgnng iaifdcghsy hvncmiekkl tacnlhs
//
LOCUS       XP_034196301             932 aa            linear   INV 03-SEP-2025
DEFINITION  WD40 repeat domain-containing protein pink isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196301
VERSION     XP_034196301.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340410.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196301.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..932
                     /product="WD40 repeat domain-containing protein pink
                     isoform X2"
                     /calculated_mol_wt=106107
     Region          28..64
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <36..>137
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          71..107
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <493..>644
                     /region_name="PspF"
                     /note="Transcriptional regulators containing an AAA-type
                     ATPase domain and a DNA-binding domain [Transcription,
                     Signal transduction mechanisms]; COG1221"
                     /db_xref="CDD:440834"
     Region          887..927
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     CDS             1..932
                     /gene="pink"
                     /coded_by="XM_034340410.2:154..2952"
                     /db_xref="GeneID:117611920"
ORIGIN      
        1 mqefpcvlse yeeinpllyk pitstqriky tcfntspnyi ilgstsgsiy lflrkpctfv
       61 qliplsegsv shvlispdek tialtttrgt vclvalkptp kllaistehi qeqinclcwn
      121 dnsseiyigd gngkvsvmvm sifmvngmfq spactlmnld savvqlnfcs plllvstltr
      181 cyicdtvqeq ykqvgnktrn gefgacfykt yltdkstlvt pkeerlvnrk gsfnfipegn
      241 snieegnfpq ifcarpssrl wevsangtvm kthqfkeala tppvticrpn mrksmyqkpt
      301 eqawmpqsin fshlftiaek ylfsytstgi yiidpitatv ilwsnefsni svaetvenei
      361 ylmtfdgnfh clilsvldal ilwlynrkry heclevcfly ktqlkqlins teinaicnie
      421 nklqilrdde lstllhplii llesnsktsp kkldsgivvv ncgnsnlkee esfrhnslpy
      481 clsqnnespc etelsegket ltnlledstn evtkikdany ntenvqksnn eerakineel
      541 ysnktitqri qadlesiyal idnikpsmde eqlekiilei dwkmnvlkds yeiltelkgf
      601 vyevlrsvel yyfnalleni svqliqpsdn etiikqimka fininaqnyk kctcgspcpt
      661 delvepkflg igryllkkii denkeqciil cnkvpymwre ylpiyvnqhg vlmndllrqc
      721 lqikdnnlls tllplldgkh wnlvatyake mqegqclfcg ksirkgnnei linwtavihe
      781 imkkqgpdva mtllikleka ipnisvdksi fqsliftkil yhhgmkhain fnkntlesse
      841 ynticstkir dqlveviekd lskpvnknif gngahhwgmh yqnklhtcpc ctlslqtpvl
      901 lgnngiaifd cghsyhvncm iekkltacnl hs
//
LOCUS       XP_034196303             779 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 6 isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196303
VERSION     XP_034196303.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340412.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..779
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform X1"
                     /calculated_mol_wt=85420
     Region          86..162
                     /region_name="RRM_CFIm68"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     cleavage factor Im 68 kDa subunit (CFIm68 or CPSF6) and
                     similar proteins; cd12643"
                     /db_xref="CDD:410048"
     Site            order(94..99,117,120..127)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410048"
     CDS             1..779
                     /gene="Cpsf6"
                     /coded_by="XM_034340412.2:124..2463"
                     /db_xref="GeneID:117611921"
ORIGIN      
        1 madgdidlya ddleqdfaqd efagdgvdly ddviaapagg nggvstgnsg dgggdttspn
       61 eetngsapyh qlgnniqpnq igrrhqlyvg nltwwtsdqd itdavqsigv sdfvevkffe
      121 nrangqskgf cvislgseqs mricmerlpk kelhgqnpvv tfptkqalnq fesqcktrpa
      181 papqqsqsqr phnphqhqpp mpphqqhpqh pqhpqhsqqn hgprmmmgpp qgvrpqrmpp
      241 pgmgppgpgg pgqqgpprmh gppmgpgpgp hhplpghpnq gppppgyqqg pwngprpngp
      301 pgpprgpsgp ggppqqgppg pgpgqhrppg mqfhggppgp pgqgpprgpp ghpggppgdp
      361 rgaqprpewn rppgmhhgpq gppgfpqhqh mqgpqpgqgp pqrgpppgsm ggmlpgpggp
      421 ppghggppqg ppqgppggpa phvnpaffpq gpphqhpgqh ppgppgpphg pphgpphgpp
      481 hgpphgpphg qphgpphvpp hgygppaaqp pygapgpdhr pegppplteq efeeimgrnr
      541 tvsssaiara vsdaaageya saietlvtai slikqskvaa ddrckiliss lqdtlrgvet
      601 ksygsarrer srsrdrersh rrrrersrsr dreyrersrd rdrerdrerd rererdrdrd
      661 reryysepyp rersrsrerd rererdreyr ersreesttr qsarprvkee ppetapvsss
      721 kasryyddry rererdrdre ressrrpser erepererer ererdrrder gdsshrsrh
//
LOCUS       XP_034196304             775 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 6 isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196304
VERSION     XP_034196304.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340413.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..775
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform X2"
                     /calculated_mol_wt=85021
     Region          86..162
                     /region_name="RRM_CFIm68"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     cleavage factor Im 68 kDa subunit (CFIm68 or CPSF6) and
                     similar proteins; cd12643"
                     /db_xref="CDD:410048"
     Site            order(94..99,117,120..127)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410048"
     CDS             1..775
                     /gene="Cpsf6"
                     /coded_by="XM_034340413.2:123..2450"
                     /db_xref="GeneID:117611921"
ORIGIN      
        1 madgdidlya ddleqdfaqd efagdgvdly ddviaapagg nggvstgnsg dgggdttspn
       61 eetngsapyh qlgnniqpnq igrrhqlyvg nltwwtsdqd itdavqsigv sdfvevkffe
      121 nrangqskgf cvislgseqs mricmerlpk kelhgqnpvv tfptkqalnq fesqcktrpa
      181 papqqsqsqr phnphqhqpp mpphqqhpqh pqhpqhsqqn hgprmmmgpp qgvrpqrmpp
      241 pgmgppgpgg pgqqgpprmh gppmgpgpgp hhplpghpnq gppppgyqqg pwngprpngp
      301 pgpprgpsgp ggppqqgppg pgpgqhrppg mqfhggppgp pgqgpprgpp ghpggppgdp
      361 rgaqprpewn rppgmhhgpq gppgfpqhqh mqgpqpgqgp pqrgpppgsm ggpggpppgh
      421 ggppqgppqg ppggpaphvn paffpqgpph qhpgqhppgp pgpphgpphg pphgpphgpp
      481 hgpphgqphg pphvpphgyg ppaaqppyga pgpdhrpegp pplteqefee imgrnrtvss
      541 saiaravsda aageyasaie tlvtaislik qskvaaddrc kilisslqdt lrgvetksyg
      601 sarrersrsr drershrrrr ersrsrdrey rersrdrdre rdrerdrere rdrdrdrery
      661 ysepyprers rsrerdrere rdreyrersr eesttrqsar prvkeeppet apvssskasr
      721 yyddryrere rdrdreress rrpsererep ererererer drrdergdss hrsrh
//
LOCUS       XP_034196305             764 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 6 isoform
            X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196305
VERSION     XP_034196305.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340414.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..764
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform X3"
                     /calculated_mol_wt=83707
     Region          71..147
                     /region_name="RRM_CFIm68"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     cleavage factor Im 68 kDa subunit (CFIm68 or CPSF6) and
                     similar proteins; cd12643"
                     /db_xref="CDD:410048"
     Site            order(79..84,102,105..112)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410048"
     CDS             1..764
                     /gene="Cpsf6"
                     /coded_by="XM_034340414.2:335..2629"
                     /db_xref="GeneID:117611921"
ORIGIN      
        1 mvladefagd gvdlyddvia apaggnggvs tgnsgdgggd ttspneetng sapyhqlgnn
       61 iqpnqigrrh qlyvgnltww tsdqditdav qsigvsdfve vkffenrang qskgfcvisl
      121 gseqsmricm erlpkkelhg qnpvvtfptk qalnqfesqc ktrpapapqq sqsqrphnph
      181 qhqppmpphq qhpqhpqhpq hsqqnhgprm mmgppqgvrp qrmpppgmgp pgpggpgqqg
      241 pprmhgppmg pgpgphhplp ghpnqgpppp gyqqgpwngp rpngppgppr gpsgpggppq
      301 qgppgpgpgq hrppgmqfhg gppgppgqgp prgppghpgg ppgdprgaqp rpewnrppgm
      361 hhgpqgppgf pqhqhmqgpq pgqgppqrgp ppgsmggmlp gpggpppghg gppqgppqgp
      421 pggpaphvnp affpqgpphq hpgqhppgpp gpphgpphgp phgpphgpph gpphgqphgp
      481 phvpphgygp paaqppygap gpdhrpegpp plteqefeei mgrnrtvsss aiaravsdaa
      541 ageyasaiet lvtaislikq skvaaddrck ilisslqdtl rgvetksygs arrersrsrd
      601 rershrrrre rsrsrdreyr ersrdrdrer drerdrerer drdrdreryy sepyprersr
      661 srerdrerer dreyrersre esttrqsarp rvkeeppeta pvssskasry yddryrerer
      721 drdreressr rpsererepe rererererd rrdergdssh rsrh
//
LOCUS       XP_034196306             757 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 6 isoform
            X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196306
VERSION     XP_034196306.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340415.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..757
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform X4"
                     /calculated_mol_wt=82010
     Region          86..162
                     /region_name="RRM_CFIm68"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     cleavage factor Im 68 kDa subunit (CFIm68 or CPSF6) and
                     similar proteins; cd12643"
                     /db_xref="CDD:410048"
     Site            order(94..99,117,120..127)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410048"
     CDS             1..757
                     /gene="Cpsf6"
                     /coded_by="XM_034340415.2:124..2397"
                     /db_xref="GeneID:117611921"
ORIGIN      
        1 madgdidlya ddleqdfaqd efagdgvdly ddviaapagg nggvstgnsg dgggdttspn
       61 eetngsapyh qlgnniqpnq igrrhqlyvg nltwwtsdqd itdavqsigv sdfvevkffe
      121 nrangqskgf cvislgseqs mricmerlpk kelhgqnpvv tfptkqalnq fesqcktrpa
      181 papqqsqsqr phnphqhqpp mpphqqhpqh pqhpqhsqqn hgprmmmgpp qgvrpqrmpp
      241 pgmgppgpgg pgqqgpprmh gppmgpgpgp hhplpghpnq gppppgyqqg pwngprpngp
      301 pgpprgpsgp ggppqqgppg pgpgqhrppg mqfhggppgp pgqgpprgpp ghpggppgdp
      361 rgaqprpewn rppgmhhgpq gppgfpqhqh mqgpqpgqgp pqrgpppgsm ggmlpgpggp
      421 ppghggppqg ppqgppggpa phvnpaffpq gpphqhpgqh ppgppgpphg pphgpphgpp
      481 hgpphgpphg qphgpphvpp hgygppaaqp pygapgpdhr pegppplteq efeeimgrnr
      541 tvsssaiara vsdaaageya saietlvtai slikqskvaa ddrckiliss lqdtlrgvet
      601 ksygsarrer srsrdrersh rrrrersrsr dreyrersrd rdrerdrerd rererdrdrd
      661 reryysepyp rersrsrerd rererdreyr ersreesttr qsarprvkee ppetapvsss
      721 kasreeatlq kayysrssrf hevhgrqsvk entietk
//
LOCUS       XP_076546613             741 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 6 isoform
            X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546613
VERSION     XP_076546613.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690498.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..741
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform X5"
                     /calculated_mol_wt=81551
     Region          86..162
                     /region_name="RRM_CFIm68"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     cleavage factor Im 68 kDa subunit (CFIm68 or CPSF6) and
                     similar proteins; cd12643"
                     /db_xref="CDD:410048"
     Site            order(94..99,117,120..127)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410048"
     CDS             1..741
                     /gene="Cpsf6"
                     /coded_by="XM_076690498.1:124..2349"
                     /db_xref="GeneID:117611921"
ORIGIN      
        1 madgdidlya ddleqdfaqd efagdgvdly ddviaapagg nggvstgnsg dgggdttspn
       61 eetngsapyh qlgnniqpnq igrrhqlyvg nltwwtsdqd itdavqsigv sdfvevkffe
      121 nrangqskgf cvislgseqs mricmerlpk kelhgqnpvv tfptkqalnq fesqcktrpa
      181 papqqsqsqr phnphqhqpp mpphqqhpqh pqhpqhsqqn hgprmmmgpp qgvrpqrmpp
      241 pgmgppgpgg pgqqgpprmh gppmgpgpgp hhplpghpnq gppppgyqqg pwngprpngp
      301 pgpprgpsgp ggppqqgppg pgpgqhrppg mqfhggppgp pgqgpprgpp ghpggppgdp
      361 rgaqprpewn rppgmlpgpg gpppghggpp qgppqgppgg paphvnpaff pqgpphqhpg
      421 qhppgppgpp hgpphgpphg pphgpphgpp hgqphgpphv pphgygppaa qppygapgpd
      481 hrpegppplt eqefeeimgr nrtvsssaia ravsdaaage yasaietlvt aislikqskv
      541 aaddrckili sslqdtlrgv etksygsarr ersrsrdrer shrrrrersr srdreyrers
      601 rdrdrerdre rdrererdrd rdreryysep yprersrsre rdrererdre yrersreest
      661 trqsarprvk eeppetapvs sskasryydd ryrererdrd reressrrps erereperer
      721 erererdrrd ergdsshrsr h
//
LOCUS       XP_034196307             737 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 6 isoform
            X6 [Osmia lignaria lignaria].
ACCESSION   XP_034196307
VERSION     XP_034196307.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340416.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..737
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform X6"
                     /calculated_mol_wt=81153
     Region          86..162
                     /region_name="RRM_CFIm68"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     cleavage factor Im 68 kDa subunit (CFIm68 or CPSF6) and
                     similar proteins; cd12643"
                     /db_xref="CDD:410048"
     Site            order(94..99,117,120..127)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410048"
     CDS             1..737
                     /gene="Cpsf6"
                     /coded_by="XM_034340416.2:123..2336"
                     /db_xref="GeneID:117611921"
ORIGIN      
        1 madgdidlya ddleqdfaqd efagdgvdly ddviaapagg nggvstgnsg dgggdttspn
       61 eetngsapyh qlgnniqpnq igrrhqlyvg nltwwtsdqd itdavqsigv sdfvevkffe
      121 nrangqskgf cvislgseqs mricmerlpk kelhgqnpvv tfptkqalnq fesqcktrpa
      181 papqqsqsqr phnphqhqpp mpphqqhpqh pqhpqhsqqn hgprmmmgpp qgvrpqrmpp
      241 pgmgppgpgg pgqqgpprmh gppmgpgpgp hhplpghpnq gppppgyqqg pwngprpngp
      301 pgpprgpsgp ggppqqgppg pgpgqhrppg mqfhggppgp pgqgpprgpp ghpggppgdp
      361 rgaqprpewn rppgpggppp ghggppqgpp qgppggpaph vnpaffpqgp phqhpgqhpp
      421 gppgpphgpp hgpphgpphg pphgpphgqp hgpphvpphg ygppaaqppy gapgpdhrpe
      481 gppplteqef eeimgrnrtv sssaiaravs daaageyasa ietlvtaisl ikqskvaadd
      541 rckilisslq dtlrgvetks ygsarrersr srdrershrr rrersrsrdr eyrersrdrd
      601 rerdrerdre rerdrdrdre ryysepypre rsrsrerdre rerdreyrer sreesttrqs
      661 arprvkeepp etapvssska sryyddryre rerdrdrere ssrrpserer eperererer
      721 erdrrdergd sshrsrh
//
LOCUS       XP_034196308             732 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage and polyadenylation specificity factor subunit 6 isoform
            X7 [Osmia lignaria lignaria].
ACCESSION   XP_034196308
VERSION     XP_034196308.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340417.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..732
                     /product="cleavage and polyadenylation specificity factor
                     subunit 6 isoform X7"
                     /calculated_mol_wt=79273
     Region          86..162
                     /region_name="RRM_CFIm68"
                     /note="RNA recognition motif (RRM) found in pre-mRNA
                     cleavage factor Im 68 kDa subunit (CFIm68 or CPSF6) and
                     similar proteins; cd12643"
                     /db_xref="CDD:410048"
     Site            order(94..99,117,120..127)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:410048"
     CDS             1..732
                     /gene="Cpsf6"
                     /coded_by="XM_034340417.2:125..2323"
                     /db_xref="GeneID:117611921"
ORIGIN      
        1 madgdidlya ddleqdfaqd efagdgvdly ddviaapagg nggvstgnsg dgggdttspn
       61 eetngsapyh qlgnniqpnq igrrhqlyvg nltwwtsdqd itdavqsigv sdfvevkffe
      121 nrangqskgf cvislgseqs mricmerlpk kelhgqnpvv tfptkqalnq fesqcktrpa
      181 papqqsqsqr phnphqhqpp mpphqqhpqh pqhpqhsqqn hgprmmmgpp qgvrpqrmpp
      241 pgmgppgpgg pgqqgpprmh gppmgpgpgp hhplpghpnq gppppgyqqg pwngprpngp
      301 pgpprgpsgp ggppqqgppg pgpgqhrppg mqfhggppgp pgqgpprgpp ghpggppgdp
      361 rgaqprpewn rppgmhhgpq gppgfpqhqh mqgpqpgqgp pqrgpppgsm ggmlpgpggp
      421 ppghggppqg ppqgppggpa phvnpaffpq gpphqhpgqh ppgppgpphg pphgpphgpp
      481 hgpphgpphg qphgpphvpp hgygppaaqp pygapgpdhr pegppplteq efeeimgrnr
      541 tvsssaiara vsdaaageya saietlvtai slikqskvaa ddrckiliss lqdtlrgvet
      601 ksygsarrer srsrdrersh rrrrersrsr dreyrersrd rdrerdrerd rererdrdrd
      661 reryysepyp rersrsrerd rererdreyr ersreerene kgrgpqraer lvpisskyqe
      721 tdeefsqstv qd
//
LOCUS       XP_034196309             694 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611922 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196309
VERSION     XP_034196309.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340418.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196309.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..694
                     /product="uncharacterized protein LOC117611922 isoform X1"
                     /calculated_mol_wt=79020
     Region          <399..556
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     CDS             1..694
                     /gene="LOC117611922"
                     /coded_by="XM_034340418.2:729..2813"
                     /db_xref="GeneID:117611922"
ORIGIN      
        1 mvvptiwirn nmlfsgeqyq erqltdaelk vqrsleklsv pdwylnkrst spkivnvdpi
       61 elrppswkcp lpknssinst ssdlsdtptk asknnnekst snnssdqpkp peskqrksss
      121 ksphtkeeif dtwvpevnlp vdisktfpkv spsrkihnin ivfppgpkvd ifdnqdckti
      181 snnysncnti rvqeqsadfs rkneespcrk knnvqevvet pvktpttrgs yrfrttstpk
      241 ftpanlfsst viedspkpkk nlsknileks sifeksfgsn dhsdfsidhc rkpkklsqdl
      301 lektfvfdrs fqsiedlstn isrdscifek nirsiepiff ekddgalstl ksssmvrdmv
      361 rklevsknss qtpqktstft wnkqapiqir kstfqlktps mfqekrfnla srnpreekpc
      421 letqspctrf lnsnrkstel envvrskhlt rekgvvqgli gvlaekvnqs tlprqkqvae
      481 vnrnfvkkvv salenadrsk neqvpktrmi sanedtgsgs dsefksytts vslkdtdssd
      541 sdqrtpspks dednrfdysy mtttsihqdd dsvywipvsr cklprtssli simsrlsgng
      601 hspcvspirs eheeqqtwgv tyrrtsslsk klfridetvv idsgysdksd rsgissryed
      661 snwsednqyd sgseckytkv krsrrksivn nfhf
//
LOCUS       XP_076546614             694 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611922 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546614
VERSION     XP_076546614.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690499.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..694
                     /product="uncharacterized protein LOC117611922 isoform X1"
                     /calculated_mol_wt=79020
     Region          <399..556
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     CDS             1..694
                     /gene="LOC117611922"
                     /coded_by="XM_076690499.1:920..3004"
                     /db_xref="GeneID:117611922"
ORIGIN      
        1 mvvptiwirn nmlfsgeqyq erqltdaelk vqrsleklsv pdwylnkrst spkivnvdpi
       61 elrppswkcp lpknssinst ssdlsdtptk asknnnekst snnssdqpkp peskqrksss
      121 ksphtkeeif dtwvpevnlp vdisktfpkv spsrkihnin ivfppgpkvd ifdnqdckti
      181 snnysncnti rvqeqsadfs rkneespcrk knnvqevvet pvktpttrgs yrfrttstpk
      241 ftpanlfsst viedspkpkk nlsknileks sifeksfgsn dhsdfsidhc rkpkklsqdl
      301 lektfvfdrs fqsiedlstn isrdscifek nirsiepiff ekddgalstl ksssmvrdmv
      361 rklevsknss qtpqktstft wnkqapiqir kstfqlktps mfqekrfnla srnpreekpc
      421 letqspctrf lnsnrkstel envvrskhlt rekgvvqgli gvlaekvnqs tlprqkqvae
      481 vnrnfvkkvv salenadrsk neqvpktrmi sanedtgsgs dsefksytts vslkdtdssd
      541 sdqrtpspks dednrfdysy mtttsihqdd dsvywipvsr cklprtssli simsrlsgng
      601 hspcvspirs eheeqqtwgv tyrrtsslsk klfridetvv idsgysdksd rsgissryed
      661 snwsednqyd sgseckytkv krsrrksivn nfhf
//
LOCUS       XP_034196310             693 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611922 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196310
VERSION     XP_034196310.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340419.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196310.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..693
                     /product="uncharacterized protein LOC117611922 isoform X2"
                     /calculated_mol_wt=78891
     Region          <398..555
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     CDS             1..693
                     /gene="LOC117611922"
                     /coded_by="XM_034340419.2:729..2810"
                     /db_xref="GeneID:117611922"
ORIGIN      
        1 mvvptiwirn nmlfsgeqye rqltdaelkv qrsleklsvp dwylnkrsts pkivnvdpie
       61 lrppswkcpl pknssinsts sdlsdtptka sknnneksts nnssdqpkpp eskqrksssk
      121 sphtkeeifd twvpevnlpv disktfpkvs psrkihnini vfppgpkvdi fdnqdcktis
      181 nnysncntir vqeqsadfsr kneespcrkk nnvqevvetp vktpttrgsy rfrttstpkf
      241 tpanlfsstv iedspkpkkn lsknilekss ifeksfgsnd hsdfsidhcr kpkklsqdll
      301 ektfvfdrsf qsiedlstni srdscifekn irsiepiffe kddgalstlk sssmvrdmvr
      361 klevsknssq tpqktstftw nkqapiqirk stfqlktpsm fqekrfnlas rnpreekpcl
      421 etqspctrfl nsnrkstele nvvrskhltr ekgvvqglig vlaekvnqst lprqkqvaev
      481 nrnfvkkvvs alenadrskn eqvpktrmis anedtgsgsd sefksyttsv slkdtdssds
      541 dqrtpspksd ednrfdysym tttsihqddd svywipvsrc klprtsslis imsrlsgngh
      601 spcvspirse heeqqtwgvt yrrtsslskk lfridetvvi dsgysdksdr sgissryeds
      661 nwsednqyds gseckytkvk rsrrksivnn fhf
//
LOCUS       XP_034196311             530 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 301a1, mitochondrial isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196311
VERSION     XP_034196311.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340420.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196311.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..530
                     /product="putative cytochrome P450 301a1, mitochondrial
                     isoform X1"
                     /calculated_mol_wt=60683
     Region          91..524
                     /region_name="CYP24A1-like"
                     /note="cytochrome P450 family 24 subfamily A, polypeptide
                     1 and similar cytochrome P450s; cd11054"
                     /db_xref="CDD:410677"
     Site            order(131,137,156,213..214,217,254,256..257,285,336,
                     339..340,343..344,405..410,515..516)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410677"
     Site            order(131,155..156,163,167,337,340..341,344..345,348,400,
                     405..406,409,411,471..474,477..481,485)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410677"
     CDS             1..530
                     /gene="Cyp301a1"
                     /coded_by="XM_034340420.2:130..1722"
                     /db_xref="GeneID:117611923"
ORIGIN      
        1 mnprmnchlq fnmirrlsqs qdilrrslcs gtvtsatrdc tvdhdaaiqa rpyeeipgpr
       61 pipilgntwr lfpvigqyqi sdmakvsqif hdeygkivrl tgligrpdll fvydadeiek
      121 iyrqegptpf rpsmpclvhy ksvvrkdffg dlpgvvgvhg epwrefrtri qkpvlqpqtv
      181 rkyitpievv tsdfikrmee ikgedgelpa dfdneihkwa lecigrvald vrlgclggnl
      241 tpdsepqkii dgakfalrnv avlelkapyw ryvptplwsr yvrnmnyfve vcmkyidaav
      301 erlktkksvn eadlslieri laketdpkma yilaldlilv gidtismavc silyqlatrp
      361 eeqekvhqel vdilpdpsvp ltnshldrav ytkafirevf rvystvigng rtlqndttis
      421 gyrvpkgvqv vfptvitgnm eeyvtdaktf kperwfkqss neklhpfasl pyghgarmcl
      481 grrfadlemq vllaklirsy kleyhhepln ykvtfmyapd gelkfkllpr
//
LOCUS       XP_034196312             427 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 301a1, mitochondrial isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196312
VERSION     XP_034196312.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340421.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196312.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="putative cytochrome P450 301a1, mitochondrial
                     isoform X2"
                     /calculated_mol_wt=48591
     Region          91..>427
                     /region_name="CYP24A1-like"
                     /note="cytochrome P450 family 24 subfamily A, polypeptide
                     1 and similar cytochrome P450s; cd11054"
                     /db_xref="CDD:410677"
     Site            order(131,137,156,213..214,217,254,256..257,285,336,
                     339..340,343..344,405..410)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410677"
     CDS             1..427
                     /gene="Cyp301a1"
                     /coded_by="XM_034340421.2:130..1413"
                     /db_xref="GeneID:117611923"
ORIGIN      
        1 mnprmnchlq fnmirrlsqs qdilrrslcs gtvtsatrdc tvdhdaaiqa rpyeeipgpr
       61 pipilgntwr lfpvigqyqi sdmakvsqif hdeygkivrl tgligrpdll fvydadeiek
      121 iyrqegptpf rpsmpclvhy ksvvrkdffg dlpgvvgvhg epwrefrtri qkpvlqpqtv
      181 rkyitpievv tsdfikrmee ikgedgelpa dfdneihkwa lecigrvald vrlgclggnl
      241 tpdsepqkii dgakfalrnv avlelkapyw ryvptplwsr yvrnmnyfve vcmkyidaav
      301 erlktkksvn eadlslieri laketdpkma yilaldlilv gidtismavc silyqlatrp
      361 eeqekvhqel vdilpdpsvp ltnshldrav ytkafirevf rvystvigng rtlqndttis
      421 gyrvpkg
//
LOCUS       XP_034196313             518 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 301a1, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034196313
VERSION     XP_034196313.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340422.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..518
                     /product="putative cytochrome P450 301a1, mitochondrial"
                     /calculated_mol_wt=60085
     Region          84..512
                     /region_name="CYP24A1-like"
                     /note="cytochrome P450 family 24 subfamily A, polypeptide
                     1 and similar cytochrome P450s; cd11054"
                     /db_xref="CDD:410677"
     Site            order(124,130,149,206..207,210,245,247..248,276,326,
                     329..330,333..334,395..400,503..504)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410677"
     Site            order(124,148..149,156,160,327,330..331,334..335,338,390,
                     395..396,399,401,459..462,465..469,473)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410677"
     CDS             1..518
                     /gene="LOC117611925"
                     /coded_by="XM_034340422.2:237..1793"
                     /db_xref="GeneID:117611925"
ORIGIN      
        1 mkmnilnyef iglrrhiikq ssniirtlet latevegqdw sqcrpysaip gprpipffgn
       61 twrfipfigd ykihavdqvs kklhneygdi vkiegllgrp dmvfiydane ierifrqeer
      121 mpyrpsmpsl nyykhvlrke ffnehagvia vhgeswynfr skvqkvmlqp qtarmyvnsi
      181 eeastaflkr irkirdknde vpddflneih kwslesiasv aldvrlgcld dnanietqql
      241 idsvttffkn vgvlelkipf wklfstptwl kyvndldtiv ritskytaaa lsriqegkns
      301 gkeasllekv lasenntkla tvlsldlflv gidttssava stlyqlalhp ekqdlaykev
      361 cnifpskdtl iegkhleqlk ylkaciketl rmypvvigng rcmtkdtiir gyrvpkgvqv
      421 vfqhyvisnl dkyfprskey lperwlqsdg vrhsfaslpf gygrrmclgr rfaelemlvv
      481 iskilqyyki eyhhekleyy inpmytpkgp lnlkfidr
//
LOCUS       XP_034196314             518 aa            linear   INV 03-SEP-2025
DEFINITION  putative cytochrome P450 301a1, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034196314
VERSION     XP_034196314.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340423.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..518
                     /product="putative cytochrome P450 301a1, mitochondrial"
                     /calculated_mol_wt=60085
     Region          84..512
                     /region_name="CYP24A1-like"
                     /note="cytochrome P450 family 24 subfamily A, polypeptide
                     1 and similar cytochrome P450s; cd11054"
                     /db_xref="CDD:410677"
     Site            order(124,130,149,206..207,210,245,247..248,276,326,
                     329..330,333..334,395..400,503..504)
                     /site_type="other"
                     /note="chemical substrate binding pocket [chemical
                     binding]"
                     /db_xref="CDD:410677"
     Site            order(124,148..149,156,160,327,330..331,334..335,338,390,
                     395..396,399,401,459..462,465..469,473)
                     /site_type="other"
                     /note="heme binding site [chemical binding]"
                     /db_xref="CDD:410677"
     CDS             1..518
                     /gene="LOC117611925"
                     /coded_by="XM_034340423.2:184..1740"
                     /db_xref="GeneID:117611925"
ORIGIN      
        1 mkmnilnyef iglrrhiikq ssniirtlet latevegqdw sqcrpysaip gprpipffgn
       61 twrfipfigd ykihavdqvs kklhneygdi vkiegllgrp dmvfiydane ierifrqeer
      121 mpyrpsmpsl nyykhvlrke ffnehagvia vhgeswynfr skvqkvmlqp qtarmyvnsi
      181 eeastaflkr irkirdknde vpddflneih kwslesiasv aldvrlgcld dnanietqql
      241 idsvttffkn vgvlelkipf wklfstptwl kyvndldtiv ritskytaaa lsriqegkns
      301 gkeasllekv lasenntkla tvlsldlflv gidttssava stlyqlalhp ekqdlaykev
      361 cnifpskdtl iegkhleqlk ylkaciketl rmypvvigng rcmtkdtiir gyrvpkgvqv
      421 vfqhyvisnl dkyfprskey lperwlqsdg vrhsfaslpf gygrrmclgr rfaelemlvv
      481 iskilqyyki eyhhekleyy inpmytpkgp lnlkfidr
//
LOCUS       XP_034196021             421 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat domain 74 lethal (2) k09848 [Osmia lignaria lignaria].
ACCESSION   XP_034196021
VERSION     XP_034196021.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340130.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196021.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="WD repeat domain 74 lethal (2) k09848"
                     /calculated_mol_wt=47970
     Region          15..324
                     /region_name="Nsa1_WDR74-like"
                     /note="Ribosome biogenesis protein Nsa1 and similar
                     proteins; cl45912"
                     /db_xref="CDD:459257"
     Region          16..40
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:439302"
     Region          43..84
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:439302"
     Region          45..86
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          92..130
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          92..127
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:439302"
     Region          130..169
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:439302"
     Region          135..168
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(168,171..173,178,196,221..224,262..265)
                     /site_type="other"
                     /note="putative rRNA binding site [nucleotide binding]"
                     /db_xref="CDD:439302"
     Region          184..230
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:439302"
     Region          184..220
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          226..263
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          233..267
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:439302"
     Region          269..311
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          270..305
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:439302"
     CDS             1..421
                     /gene="l(2)k09848"
                     /coded_by="XM_034340130.2:128..1393"
                     /db_xref="GeneID:117611812"
ORIGIN      
        1 mevkdnfhiy vgcksgafkg iriepeknvt rniqnlvfik dndevtamsw gddeekevli
       61 acgtkeigsi kvydtdyttf tcsftcdagr grvngisryd egiltavesg evklykynek
      121 eeilvnagan lrkmrhskin kkmiatggfe hylklfdlek qkqvfqaknv ppdwlqlrvp
      181 vwisdmdfip ttenivtvgr yghvsvydpk tqrrpviqlq lkdeaittls tmprekqiii
      241 gtgkgkmklv dlrkpskvln tykgflgsvt giacstsepy vvsvsldryl rihhidtkql
      301 lrkvyltsri ssillhsgfs lpkennvned srkntngvde ylkeehdnvp rdqnsdtdyd
      361 ilfdkmqvvg dkkhkalter krtnsrndlt idneiifhks plnkneklpk sskkqkttnl
      421 i
//
LOCUS       XP_034196022             389 aa            linear   INV 03-SEP-2025
DEFINITION  nucleosome assembly protein 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196022
VERSION     XP_034196022.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196022.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="nucleosome assembly protein 1 isoform X1"
                     /calculated_mol_wt=44653
     Region          63..336
                     /region_name="NAP"
                     /note="Nucleosome assembly protein (NAP); pfam00956"
                     /db_xref="CDD:460010"
     CDS             1..389
                     /gene="Nap1"
                     /coded_by="XM_034340131.2:159..1328"
                     /db_xref="GeneID:117611813"
ORIGIN      
        1 mttdperagd ksdmesvede ddnntsefts qilrrpdvla vlqdrfsapg leqmqnlpas
       61 vkrrikalkq lqlvttniea kfyeevhale ckyykmcasl yekrseivsg vheptdeqcv
      121 wesdddeegl tndlkskvkl eddkeqkkee epneetkgip dfwltifknv gtladmvqeh
      181 depilkhlyd ikviflesnp mgfvlefhfe pneyftnsvl tkeyimkctp dkddpfsfeg
      241 peiykckgcv idwkkgknvt vktikknqkh ksrgsmrtvt ktvqndsffn ffnppvvpdd
      301 aeaelddetq alltsdfeig hyirerivpr avlyytgegl edededyeee egdeddpede
      361 deddeesspt napsggklqg nepneckqq
//
LOCUS       XP_034196023             389 aa            linear   INV 03-SEP-2025
DEFINITION  nucleosome assembly protein 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196023
VERSION     XP_034196023.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340132.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196023.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="nucleosome assembly protein 1 isoform X1"
                     /calculated_mol_wt=44653
     Region          63..336
                     /region_name="NAP"
                     /note="Nucleosome assembly protein (NAP); pfam00956"
                     /db_xref="CDD:460010"
     CDS             1..389
                     /gene="Nap1"
                     /coded_by="XM_034340132.2:132..1301"
                     /db_xref="GeneID:117611813"
ORIGIN      
        1 mttdperagd ksdmesvede ddnntsefts qilrrpdvla vlqdrfsapg leqmqnlpas
       61 vkrrikalkq lqlvttniea kfyeevhale ckyykmcasl yekrseivsg vheptdeqcv
      121 wesdddeegl tndlkskvkl eddkeqkkee epneetkgip dfwltifknv gtladmvqeh
      181 depilkhlyd ikviflesnp mgfvlefhfe pneyftnsvl tkeyimkctp dkddpfsfeg
      241 peiykckgcv idwkkgknvt vktikknqkh ksrgsmrtvt ktvqndsffn ffnppvvpdd
      301 aeaelddetq alltsdfeig hyirerivpr avlyytgegl edededyeee egdeddpede
      361 deddeesspt napsggklqg nepneckqq
//
LOCUS       XP_034196024             389 aa            linear   INV 03-SEP-2025
DEFINITION  nucleosome assembly protein 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196024
VERSION     XP_034196024.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340133.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196024.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="nucleosome assembly protein 1 isoform X1"
                     /calculated_mol_wt=44653
     Region          63..336
                     /region_name="NAP"
                     /note="Nucleosome assembly protein (NAP); pfam00956"
                     /db_xref="CDD:460010"
     CDS             1..389
                     /gene="Nap1"
                     /coded_by="XM_034340133.2:145..1314"
                     /db_xref="GeneID:117611813"
ORIGIN      
        1 mttdperagd ksdmesvede ddnntsefts qilrrpdvla vlqdrfsapg leqmqnlpas
       61 vkrrikalkq lqlvttniea kfyeevhale ckyykmcasl yekrseivsg vheptdeqcv
      121 wesdddeegl tndlkskvkl eddkeqkkee epneetkgip dfwltifknv gtladmvqeh
      181 depilkhlyd ikviflesnp mgfvlefhfe pneyftnsvl tkeyimkctp dkddpfsfeg
      241 peiykckgcv idwkkgknvt vktikknqkh ksrgsmrtvt ktvqndsffn ffnppvvpdd
      301 aeaelddetq alltsdfeig hyirerivpr avlyytgegl edededyeee egdeddpede
      361 deddeesspt napsggklqg nepneckqq
//
LOCUS       XP_034196025             389 aa            linear   INV 03-SEP-2025
DEFINITION  nucleosome assembly protein 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196025
VERSION     XP_034196025.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340134.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196025.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="nucleosome assembly protein 1 isoform X1"
                     /calculated_mol_wt=44653
     Region          63..336
                     /region_name="NAP"
                     /note="Nucleosome assembly protein (NAP); pfam00956"
                     /db_xref="CDD:460010"
     CDS             1..389
                     /gene="Nap1"
                     /coded_by="XM_034340134.2:27..1196"
                     /db_xref="GeneID:117611813"
ORIGIN      
        1 mttdperagd ksdmesvede ddnntsefts qilrrpdvla vlqdrfsapg leqmqnlpas
       61 vkrrikalkq lqlvttniea kfyeevhale ckyykmcasl yekrseivsg vheptdeqcv
      121 wesdddeegl tndlkskvkl eddkeqkkee epneetkgip dfwltifknv gtladmvqeh
      181 depilkhlyd ikviflesnp mgfvlefhfe pneyftnsvl tkeyimkctp dkddpfsfeg
      241 peiykckgcv idwkkgknvt vktikknqkh ksrgsmrtvt ktvqndsffn ffnppvvpdd
      301 aeaelddetq alltsdfeig hyirerivpr avlyytgegl edededyeee egdeddpede
      361 deddeesspt napsggklqg nepneckqq
//
LOCUS       XP_034196026             336 aa            linear   INV 03-SEP-2025
DEFINITION  nucleosome assembly protein 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196026
VERSION     XP_034196026.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340135.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..336
                     /product="nucleosome assembly protein 1 isoform X2"
                     /calculated_mol_wt=38714
     Region          10..283
                     /region_name="NAP"
                     /note="Nucleosome assembly protein (NAP); pfam00956"
                     /db_xref="CDD:460010"
     CDS             1..336
                     /gene="Nap1"
                     /coded_by="XM_034340135.2:117..1127"
                     /db_xref="GeneID:117611813"
ORIGIN      
        1 mqnlpasvkr rikalkqlql vttnieakfy eevhalecky ykmcaslyek rseivsgvhe
       61 ptdeqcvwes dddeegltnd lkskvkledd keqkkeeepn eetkgipdfw ltifknvgtl
      121 admvqehdep ilkhlydikv iflesnpmgf vlefhfepne yftnsvltke yimkctpdkd
      181 dpfsfegpei ykckgcvidw kkgknvtvkt ikknqkhksr gsmrtvtktv qndsffnffn
      241 ppvvpddaea elddetqall tsdfeighyi rerivpravl yytgeglede dedyeeeegd
      301 eddpededed deessptnap sggklqgnep neckqq
//
LOCUS       XP_034196318             350 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611926 [Osmia lignaria lignaria].
ACCESSION   XP_034196318
VERSION     XP_034196318.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340427.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196318.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="uncharacterized protein LOC117611926"
                     /calculated_mol_wt=41446
     CDS             1..350
                     /gene="LOC117611926"
                     /coded_by="XM_034340427.2:251..1303"
                     /db_xref="GeneID:117611926"
ORIGIN      
        1 malrltnsrs ksasdileet trnkkkfeqh yftenryrtf tlkgfeeekt sfvekeqstp
       61 kstqnfvkkl iaslekknea ckaddeffak yytrndcpnk tdncrkenlt ienkipvpgk
      121 svtgnnncvn ktplrnlnrn eckindqkly cnryhtfqsv yesnvrknee kkygfssfdr
      181 nryrverkqm eivkkeeent errtkfvrsk sfkdffgsml kwrrsrnkkp qhfmscdhli
      241 eqsknlnsrn gqilsrssss lqatvlqdcd dkniirnker ratirpiygg rrneqlleil
      301 skkkedlent ehllkpsaik diakcfnqlp nkslinnqln errklhvkkh
//
LOCUS       XP_034196027             160 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196027
VERSION     XP_034196027.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340136.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..160
                     /product="uncharacterized protein LOC117611814 isoform X1"
                     /calculated_mol_wt=18973
     CDS             1..160
                     /gene="LOC117611814"
                     /coded_by="XM_034340136.2:1837..2319"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv feipvhdiqs lndtqheeiq
      121 eeeeeikias rkfklkngir ilnektffnr klkeqrnsvc
//
LOCUS       XP_034196028             155 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196028
VERSION     XP_034196028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="uncharacterized protein LOC117611814 isoform X2"
                     /calculated_mol_wt=18387
     CDS             1..155
                     /gene="LOC117611814"
                     /coded_by="XM_034340137.2:1837..2304"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv hdiqslndtq heeiqeeeee
      121 ikiasrkfkl kngirilnek tffnrklkeq rnsvc
//
LOCUS       XP_034196029             149 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196029
VERSION     XP_034196029.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="uncharacterized protein LOC117611814 isoform X3"
                     /calculated_mol_wt=17641
     CDS             1..149
                     /gene="LOC117611814"
                     /coded_by="XM_034340138.2:1837..2286"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv feipvhdiqs lndtqhevfr
      121 mniihmttrk svsnlydpik tcdrenffq
//
LOCUS       XP_076546615             146 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546615
VERSION     XP_076546615.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690500.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..146
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..146
                     /product="uncharacterized protein LOC117611814 isoform X4"
                     /calculated_mol_wt=17238
     CDS             1..146
                     /gene="LOC117611814"
                     /coded_by="XM_076690500.1:1837..2277"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv feipvhdiqs lndtqhemni
      121 ihmttrksvs nlydpiktcd renffq
//
LOCUS       XP_076546616             145 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546616
VERSION     XP_076546616.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690501.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..145
                     /product="uncharacterized protein LOC117611814 isoform X5"
                     /calculated_mol_wt=17035
     CDS             1..145
                     /gene="LOC117611814"
                     /coded_by="XM_076690501.1:1837..2274"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv feipvhdiqs lndtqheeiq
      121 eeeeeikias rkfklkngir ilngn
//
LOCUS       XP_034196031             144 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196031
VERSION     XP_034196031.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340140.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..144
                     /product="uncharacterized protein LOC117611814 isoform X6"
                     /calculated_mol_wt=17055
     CDS             1..144
                     /gene="LOC117611814"
                     /coded_by="XM_034340140.2:1837..2271"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv hdiqslndtq hevfrmniih
      121 mttrksvsnl ydpiktcdre nffq
//
LOCUS       XP_076546617             140 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546617
VERSION     XP_076546617.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690502.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="uncharacterized protein LOC117611814 isoform X7"
                     /calculated_mol_wt=16449
     CDS             1..140
                     /gene="LOC117611814"
                     /coded_by="XM_076690502.1:1837..2259"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv hdiqslndtq heeiqeeeee
      121 ikiasrkfkl kngirilngn
//
LOCUS       XP_034196035             127 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196035
VERSION     XP_034196035.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340144.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..127
                     /product="uncharacterized protein LOC117611814 isoform X8"
                     /calculated_mol_wt=14939
     CDS             1..127
                     /gene="LOC117611814"
                     /coded_by="XM_034340144.2:1837..2220"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv feipvhdiqs lndtqheklk
      121 eqrnsvc
//
LOCUS       XP_034196036             122 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196036
VERSION     XP_034196036.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340145.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..122
                     /product="uncharacterized protein LOC117611814 isoform X9"
                     /calculated_mol_wt=14419
     CDS             1..122
                     /gene="LOC117611814"
                     /coded_by="XM_034340145.2:1837..2205"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv feipvhdiqs lndtqheenf
      121 fq
//
LOCUS       XP_034196037             122 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196037
VERSION     XP_034196037.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340146.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..122
                     /product="uncharacterized protein LOC117611814 isoform
                     X10"
                     /calculated_mol_wt=14354
     CDS             1..122
                     /gene="LOC117611814"
                     /coded_by="XM_034340146.2:1837..2205"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv hdiqslndtq heklkeqrns
      121 vc
//
LOCUS       XP_034196038             118 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X11 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196038
VERSION     XP_034196038.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340147.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..118
                     /product="uncharacterized protein LOC117611814 isoform
                     X11"
                     /calculated_mol_wt=13882
     CDS             1..118
                     /gene="LOC117611814"
                     /coded_by="XM_034340147.2:1837..2193"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv feipvhdiqs lndtqhee
//
LOCUS       XP_034196040             117 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X12 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196040
VERSION     XP_034196040.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340149.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..117
                     /product="uncharacterized protein LOC117611814 isoform
                     X12"
                     /calculated_mol_wt=13833
     CDS             1..117
                     /gene="LOC117611814"
                     /coded_by="XM_034340149.2:1837..2190"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv hdiqslndtq heenffq
//
LOCUS       XP_034196041             113 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611814 isoform X13 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196041
VERSION     XP_034196041.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340150.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..113
                     /product="uncharacterized protein LOC117611814 isoform
                     X13"
                     /calculated_mol_wt=13296
     CDS             1..113
                     /gene="LOC117611814"
                     /coded_by="XM_034340150.2:1837..2178"
                     /db_xref="GeneID:117611814"
ORIGIN      
        1 msdrilrklf swqeawiscr elflarivhd ddddrrrggg rsssnrrnte ssqrrfgysv
       61 irfakedese gssrrdrsng dnyldlkerr spfvdfelsv hdiqslndtq hee
//
LOCUS       XP_034195522             191 aa            linear   INV 03-SEP-2025
DEFINITION  peroxiredoxin 5 [Osmia lignaria lignaria].
ACCESSION   XP_034195522
VERSION     XP_034195522.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339631.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..191
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..191
                     /product="peroxiredoxin 5"
                     /calculated_mol_wt=20335
     Region          40..189
                     /region_name="PRX5_like"
                     /note="Peroxiredoxin (PRX) family, PRX5-like subfamily;
                     members are similar to the human protein, PRX5, a
                     homodimeric TRX peroxidase, widely expressed in tissues
                     and found cellularly in mitochondria, peroxisomes and the
                     cytosol. The cellular location of PRX5...; cd03013"
                     /db_xref="CDD:239311"
     Site            order(79,82,159)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239311"
     Site            order(80,112,114,135..136,156)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239311"
     CDS             1..191
                     /gene="Prx5"
                     /coded_by="XM_034339631.2:256..831"
                     /db_xref="GeneID:117611620"
ORIGIN      
        1 mnqlinrivc asqqallskk lctvtvrgfr vsaqnmviav gdklpaidlf edtptnkvnl
       61 aklaadkkiv vfgvpgaftp gcskthlpgy iqkaqelksk giaeivcisv ndpfvmaawg
      121 kehgaqgkvr mladpaaqft dalglstdlp vlggkrskrf smllnkgvvq elniepdntg
      181 lscslaenlk m
//
LOCUS       XP_034191671             164 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609437 [Osmia lignaria lignaria].
ACCESSION   XP_034191671
VERSION     XP_034191671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..164
                     /product="uncharacterized protein LOC117609437"
                     /calculated_mol_wt=18629
     CDS             1..164
                     /gene="LOC117609437"
                     /coded_by="XM_034335780.2:440..934"
                     /db_xref="GeneID:117609437"
ORIGIN      
        1 mfrpvdrpls frqrasewiy kmlflwsrep apgpkagpwf awfatalviw rcrrsvakai
       61 lavspfrllk krhglrfats rasstvtvkq hprlklgnsi lqrkhkhtsi ktkakrlctg
      121 dgphvtnsts amqqihyrmt rsgkiygkyp nkvampancn qgsl
//
LOCUS       XP_034195571             881 aa            linear   INV 03-SEP-2025
DEFINITION  paramyosin, long form [Osmia lignaria lignaria].
ACCESSION   XP_034195571
VERSION     XP_034195571.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339680.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="paramyosin, long form"
                     /calculated_mol_wt=102777
     Region          <34..856
                     /region_name="Myosin_tail_1"
                     /note="Myosin tail; pfam01576"
                     /db_xref="CDD:460256"
     CDS             1..881
                     /gene="LOC117611651"
                     /coded_by="XM_034339680.2:285..2930"
                     /db_xref="GeneID:117611651"
ORIGIN      
        1 mnqtdrmssa iakaskytyr stgggtadvs ieysadlsal trledkirll qddleserel
       61 rqriererad lsvqviqlse rleeaeggae sqfeinkkrd telaklrkll edvhleseet
      121 ahllrkkhqe vvvdfqdqid qlskararad kekskfqqev yellaqldsv tkeklmsikt
      181 vekleihvae lnvkieelnr tivditshkt rlsqenielt kevqdlkvni enviylktqi
      241 agqledarrr lededrrrsl veaslhqvet elesvriqle eesearldie rqlvkangev
      301 qiwrskyete anaraeevee lrrkysariq eqeeqietll vkinnlekqk srlqsevevl
      361 iidlekangt arelqkrveq lekinielka rldesmamye qsqrdlrnkq qelqrtnael
      421 dktrelkdql arenkkladd lsdaknqlad mnrrlhelel elrrlenere elaaaykeae
      481 agrkieeqrs qrlsaeltql rhdyerrlte kdeeiecirk qtsieieqln arvveaetkl
      541 ktevqrvkkk lqiqitelel tldvanknni dlqktikkqs ltltelqahy devqrqlqvt
      601 ldqlgisqrr lqsltaelee vrgnyesalr akrsveqqye esvsrinelt tinvniassk
      661 akleqelatl agdyeevtke lrvsderyqr vqnelkhtve ilheeqeriv kieaikksle
      721 ievknlsvrl eeveanaivg gkriisklea rirdleleld eekrrhaetv kilrkkerni
      781 kevmiqveed skniallqes ldkahqkvsi ykrqlqeqeg msqqsvtrvr rfqreleaae
      841 dradtaesnl tlirakhrsf vttstvpgsq vylvqetrsd l
//
LOCUS       XP_034195572             850 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 53 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195572
VERSION     XP_034195572.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339681.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..850
                     /product="vacuolar protein sorting 53 isoform X1"
                     /calculated_mol_wt=97183
     Region          53..453
                     /region_name="Vps53_N"
                     /note="Vps53-like, N-terminal; pfam04100"
                     /db_xref="CDD:427710"
     CDS             1..850
                     /gene="Vps53"
                     /coded_by="XM_034339681.2:231..2783"
                     /db_xref="GeneID:117611652"
ORIGIN      
        1 mncmieeiev kakmegheed dlddlnstvy tfppnvqnvi eqvlpstdpl dqpnfnvvdy
       61 inslfpteqs lsniddvvnn melkihtidk eirsvvrgqt nvgqdgraal edaqkvikql
      121 fvhikdikdk aeqseevvke itrdikqldf akrnltasit alnhlhmlve gvdtlkiltq
      181 kkqygeivlp lqavmevmqh fnsymdipqv kqlsdevhqi hvelaqqita dfkqafsgqn
      241 pkyfsqlteg clvlsvldpk vkkdlltwfv giqlqeyahl fdenqdfawl dkidrryawi
      301 kkhlldfesk fatifpqdwe iseriavqfc hvtredltkl mhkrrseidv klllyaiqrt
      361 snfesllakr fsgvtlenve tvnkkntpni naidnkvpgn pfeeneeaqi ekpkpspfan
      421 ligkcfepyl siyiesldrn ladlmdkfvs dsktqppgak dfdgiegpss vlsscadlfv
      481 fykkcmlqct qlstgsimln laetfqkylr eyalkvlqnn lpkiggsagi atsmssitrd
      541 frdlstsgfi qnfqsflkeg entkfskeeq sriccvltta eyclettqql eeklrertdk
      601 cyaekinlsq eqdifhnvis nciqllvqdl esacdsalta mtkiqwssie vvgdqsnyvn
      661 tivahlrqti ptirdrlfsc rkyftqlcvk fassfivklv qqlykckpln tvgaeqllld
      721 vhmlktalld lpstgyqiqr kapatytkvv ikgmataemi lkivmspies pkdfvkqcrm
      781 rlpdlqapef qkildmkglk kteqvllleq fkqpenidit hdnrshitqd tpeheagrik
      841 rleklikkri
//
LOCUS       XP_076546618             811 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 53 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546618
VERSION     XP_076546618.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690503.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..811
                     /product="vacuolar protein sorting 53 isoform X2"
                     /calculated_mol_wt=92580
     Region          53..453
                     /region_name="Vps53_N"
                     /note="Vps53-like, N-terminal; pfam04100"
                     /db_xref="CDD:427710"
     CDS             1..811
                     /gene="Vps53"
                     /coded_by="XM_076690503.1:320..2755"
                     /db_xref="GeneID:117611652"
ORIGIN      
        1 mncmieeiev kakmegheed dlddlnstvy tfppnvqnvi eqvlpstdpl dqpnfnvvdy
       61 inslfpteqs lsniddvvnn melkihtidk eirsvvrgqt nvgqdgraal edaqkvikql
      121 fvhikdikdk aeqseevvke itrdikqldf akrnltasit alnhlhmlve gvdtlkiltq
      181 kkqygeivlp lqavmevmqh fnsymdipqv kqlsdevhqi hvelaqqita dfkqafsgqn
      241 pkyfsqlteg clvlsvldpk vkkdlltwfv giqlqeyahl fdenqdfawl dkidrryawi
      301 kkhlldfesk fatifpqdwe iseriavqfc hvtredltkl mhkrrseidv klllyaiqrt
      361 snfesllakr fsgvtlenve tvnkkntpni naidnkvpgn pfeeneeaqi ekpkpspfan
      421 ligkcfepyl siyiesldrn ladlmdkfvs dsktqppgak dfdgiegpss vlsscadlfv
      481 fykkcmlqct qlstgsimln laetfqkylr eyalkvlqnn lpkiggsagi atsmssitrd
      541 frdlstsgfi qnfqsflkeg entkfskeeq sriccvltta eyclettqql eeklrertdk
      601 cyaekinlsq eqdifhnvis nciqllvqdl esacdsalta mtkiqwssie vvgdqsnyvn
      661 tivahlrqti ptirdrlfsc rkyftqlcvk fassfivklv qqlykckpln tvgaeqllld
      721 vhmlktalld lpstgyqiqr kapatytkvv ikgmataemi lkivmspies pkdfvkqcrm
      781 rlpdlqapef qkildmkvrf sfvlsilyln l
//
LOCUS       XP_034195573             522 aa            linear   INV 03-SEP-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195573
VERSION     XP_034195573.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339682.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="TGF-beta receptor type-1 babo isoform X1"
                     /calculated_mol_wt=58846
     Region          31..112
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          195..224
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          228..514
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(230..234,238,249,251,279,299..302,306,308,352,354,
                     356..357,359,370,373,394..397)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(230..236,238,249,251,299..300,302,306,356..357,359,
                     370)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(234,306,308,352,354,356,373,394..397)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(261..262,265..266,269..270,284,286)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            order(369..387,389..397)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..522
                     /gene="babo"
                     /coded_by="XM_034339682.2:857..2425"
                     /db_xref="GeneID:117611653"
ORIGIN      
        1 mrgacdggia sflalallcl fhtsclvdgl lkcfcdickd nnqtcetdgy cfastslend
       61 tpmyarrcyd kvhffpapey siwcsmnstl rgpgdveyvv accdhsdfcn rdlrpsfpsr
      121 efrggpysri tdylgrvpsi gddatwvtwk maltiampic aicvvvmviy hvymtkpptp
      181 pdpddsdrpi ldgvtirdml ekttsgsgsv glpllvqrsi arqiqlveti gkgrfgevwr
      241 grwrgenvav kifssreers wfreveiyqt vmlrhdnilg fiaadnkdng twtqlwlitd
      301 yhekgslfdy lnvstvdtng mirmalsitt glahlhmeiv gtqgkpaiah rdlksknilv
      361 ktngtcaigd lglavrhdvr tntvdiqlnn nrvgtkryma pevleetvnm nhfesfkrad
      421 vyalglilwe iarrcnvggi hdeyqlpfyd lvpsdptiee mrkvvctdrq rpsipnrwqs
      481 iealqvmskv mkecwyhnaa arltalrikk slanygamed lk
//
LOCUS       XP_034195575             521 aa            linear   INV 03-SEP-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195575
VERSION     XP_034195575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..521
                     /product="TGF-beta receptor type-1 babo isoform X2"
                     /calculated_mol_wt=58747
     Region          31..112
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          195..223
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          227..513
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(229..233,237,248,250,278,298..301,305,307,351,353,
                     355..356,358,369,372,393..396)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(229..235,237,248,250,298..299,301,305,355..356,358,
                     369)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(233,305,307,351,353,355,372,393..396)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(260..261,264..265,268..269,283,285)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            order(368..386,388..396)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..521
                     /gene="babo"
                     /coded_by="XM_034339684.2:857..2422"
                     /db_xref="GeneID:117611653"
ORIGIN      
        1 mrgacdggia sflalallcl fhtsclvdgl lkcfcdickd nnqtcetdgy cfastslend
       61 tpmyarrcyd kvhffpapey siwcsmnstl rgpgdveyvv accdhsdfcn rdlrpsfpsr
      121 efrggpysri tdylgrvpsi gddatwvtwk maltiampic aicvvvmviy hvymtkpptp
      181 pdpddsdrpi ldgvtirdml ekttsgsgsg lpllvqrsia rqiqlvetig kgrfgevwrg
      241 rwrgenvavk ifssreersw freveiyqtv mlrhdnilgf iaadnkdngt wtqlwlitdy
      301 hekgslfdyl nvstvdtngm irmalsittg lahlhmeivg tqgkpaiahr dlksknilvk
      361 tngtcaigdl glavrhdvrt ntvdiqlnnn rvgtkrymap evleetvnmn hfesfkradv
      421 yalglilwei arrcnvggih deyqlpfydl vpsdptieem rkvvctdrqr psipnrwqsi
      481 ealqvmskvm kecwyhnaaa rltalrikks lanygamedl k
//
LOCUS       XP_034195576             509 aa            linear   INV 03-SEP-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195576
VERSION     XP_034195576.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339685.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..509
                     /product="TGF-beta receptor type-1 babo isoform X3"
                     /calculated_mol_wt=57409
     Region          31..112
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          182..211
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          215..501
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(217..221,225,236,238,266,286..289,293,295,339,341,
                     343..344,346,357,360,381..384)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(217..223,225,236,238,286..287,289,293,343..344,346,
                     357)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(221,293,295,339,341,343,360,381..384)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(248..249,252..253,256..257,271,273)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            order(356..374,376..384)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..509
                     /gene="babo"
                     /coded_by="XM_034339685.2:857..2386"
                     /db_xref="GeneID:117611653"
ORIGIN      
        1 mrgacdggia sflalallcl fhtsclvdgl lkcfcdickd nnqtcetdgy cfastslend
       61 tpmyarrcyd kvhffpapey siwcsmnstl rgpgdveyvv accdhsdfcn rdlrpsfpsr
      121 efrvpsigdd atwvtwkmal tiampicaic vvvmviyhvy mtkpptppdp ddsdrpildg
      181 vtirdmlekt tsgsgsvglp llvqrsiarq iqlvetigkg rfgevwrgrw rgenvavkif
      241 ssreerswfr eveiyqtvml rhdnilgfia adnkdngtwt qlwlitdyhe kgslfdylnv
      301 stvdtngmir malsittgla hlhmeivgtq gkpaiahrdl ksknilvktn gtcaigdlgl
      361 avrhdvrtnt vdiqlnnnrv gtkrymapev leetvnmnhf esfkradvya lglilweiar
      421 rcnvggihde yqlpfydlvp sdptieemrk vvctdrqrps ipnrwqsiea lqvmskvmke
      481 cwyhnaaarl talrikksla nygamedlk
//
LOCUS       XP_034195577             505 aa            linear   INV 03-SEP-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195577
VERSION     XP_034195577.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339686.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="TGF-beta receptor type-1 babo isoform X4"
                     /calculated_mol_wt=56870
     Region          31..104
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          178..207
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          211..497
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(213..217,221,232,234,262,282..285,289,291,335,337,
                     339..340,342,353,356,377..380)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(213..219,221,232,234,282..283,285,289,339..340,342,
                     353)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(217,289,291,335,337,339,356,377..380)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(244..245,248..249,252..253,267,269)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            order(352..370,372..380)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..505
                     /gene="babo"
                     /coded_by="XM_034339686.2:857..2374"
                     /db_xref="GeneID:117611653"
ORIGIN      
        1 mrgacdggia sflalallcl fhtsclvdgl lkcfcdickd nnqtcetdgy cfastslend
       61 tpmyarrcld rkrwflpeep licnskpgiy wsslccnkdy cnqnlypvll ssdspdlssv
      121 psigddatwv twkmaltiam picaicvvvm viyhvymtkp ptppdpddsd rpildgvtir
      181 dmlekttsgs gsvglpllvq rsiarqiqlv etigkgrfge vwrgrwrgen vavkifssre
      241 erswfrevei yqtvmlrhdn ilgfiaadnk dngtwtqlwl itdyhekgsl fdylnvstvd
      301 tngmirmals ittglahlhm eivgtqgkpa iahrdlkskn ilvktngtca igdlglavrh
      361 dvrtntvdiq lnnnrvgtkr ymapevleet vnmnhfesfk radvyalgli lweiarrcnv
      421 ggihdeyqlp fydlvpsdpt ieemrkvvct drqrpsipnr wqsiealqvm skvmkecwyh
      481 naaarltalr ikkslanyga medlk
//
LOCUS       XP_034195578             503 aa            linear   INV 03-SEP-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034195578
VERSION     XP_034195578.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..503
                     /product="TGF-beta receptor type-1 babo isoform X5"
                     /calculated_mol_wt=56654
     Region          31..107
                     /region_name="TFP_LU_ECD_Babo"
                     /note="extracellular domain (ECD) found in Drosophila
                     melanogaster Baboon and similar proteins; cd23598"
                     /db_xref="CDD:467127"
     Region          176..205
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          209..495
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(211..215,219,230,232,260,280..283,287,289,333,335,
                     337..338,340,351,354,375..378)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(211..217,219,230,232,280..281,283,287,337..338,340,
                     351)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(215,287,289,333,335,337,354,375..378)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(242..243,246..247,250..251,265,267)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            order(350..368,370..378)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..503
                     /gene="babo"
                     /coded_by="XM_034339687.2:856..2367"
                     /db_xref="GeneID:117611653"
ORIGIN      
        1 mrgacdggia sflalallcl fhtsclvdgl lkcfcdickd nnqtcetdgy cfastslend
       61 tpmyarrcvn kqlspsqpep lifcmtsdsr nttfviecck edfcnrdlkp plhprkevps
      121 igddatwvtw kmaltiampi caicvvvmvi yhvymtkppt ppdpddsdrp ildgvtirdm
      181 lekttsgsgs vglpllvqrs iarqiqlvet igkgrfgevw rgrwrgenva vkifssreer
      241 swfreveiyq tvmlrhdnil gfiaadnkdn gtwtqlwlit dyhekgslfd ylnvstvdtn
      301 gmirmalsit tglahlhmei vgtqgkpaia hrdlksknil vktngtcaig dlglavrhdv
      361 rtntvdiqln nnrvgtkrym apevleetvn mnhfesfkra dvyalglilw eiarrcnvgg
      421 ihdeyqlpfy dlvpsdptie emrkvvctdr qrpsipnrwq siealqvmsk vmkecwyhna
      481 aarltalrik kslanygame dlk
//
LOCUS       XP_034195579             437 aa            linear   INV 03-SEP-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195579
VERSION     XP_034195579.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339688.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..437
                     /product="TGF-beta receptor type-1 babo isoform X6"
                     /calculated_mol_wt=49381
     Region          <14..33
                     /region_name="TFP"
                     /note="three-fingered protein (TFP) fold found in Ly6/uPAR
                     (LU) and snake toxin superfamily; cl45932"
                     /db_xref="CDD:480272"
     Region          110..139
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          143..429
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(145..149,153,164,166,194,214..217,221,223,267,269,
                     271..272,274,285,288,309..312)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(145..151,153,164,166,214..215,217,221,271..272,274,
                     285)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(149,221,223,267,269,271,288,309..312)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(176..177,180..181,184..185,199,201)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            order(284..302,304..312)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..437
                     /gene="babo"
                     /coded_by="XM_034339688.2:6692..8005"
                     /db_xref="GeneID:117611653"
ORIGIN      
        1 mnstlrgpgd veyvvaccdh sdfcnrdlrp sfpsrefrgg pysritdylg rvpsigddat
       61 wvtwkmalti ampicaicvv vmviyhvymt kpptppdpdd sdrpildgvt irdmlektts
      121 gsgsvglpll vqrsiarqiq lvetigkgrf gevwrgrwrg envavkifss reerswfrev
      181 eiyqtvmlrh dnilgfiaad nkdngtwtql wlitdyhekg slfdylnvst vdtngmirma
      241 lsittglahl hmeivgtqgk paiahrdlks knilvktngt caigdlglav rhdvrtntvd
      301 iqlnnnrvgt krymapevle etvnmnhfes fkradvyalg lilweiarrc nvggihdeyq
      361 lpfydlvpsd ptieemrkvv ctdrqrpsip nrwqsiealq vmskvmkecw yhnaaarlta
      421 lrikkslany gamedlk
//
LOCUS       XP_034195580             405 aa            linear   INV 03-SEP-2025
DEFINITION  TGF-beta receptor type-1 babo isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034195580
VERSION     XP_034195580.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339689.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="TGF-beta receptor type-1 babo isoform X7"
                     /calculated_mol_wt=45594
     Region          78..107
                     /region_name="GS"
                     /note="GS motif; smart00467"
                     /db_xref="CDD:197743"
     Region          111..397
                     /region_name="STKc_TGFbR1_ACVR1b_ACVR1c"
                     /note="Catalytic domain of the Serine/Threonine Kinases,
                     Transforming Growth Factor beta Type I Receptor and
                     Activin Type IB/IC Receptors; cd14143"
                     /db_xref="CDD:271045"
     Site            order(113..117,121,132,134,162,182..185,189,191,235,237,
                     239..240,242,253,256,277..280)
                     /site_type="active"
                     /db_xref="CDD:271045"
     Site            order(113..119,121,132,134,182..183,185,189,239..240,242,
                     253)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271045"
     Site            order(117,189,191,235,237,239,256,277..280)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271045"
     Site            order(144..145,148..149,152..153,167,169)
                     /site_type="other"
                     /note="FKBP12 binding site [polypeptide binding]"
                     /db_xref="CDD:271045"
     Site            order(252..270,272..280)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271045"
     CDS             1..405
                     /gene="babo"
                     /coded_by="XM_034339689.2:997..2214"
                     /db_xref="GeneID:117611653"
ORIGIN      
        1 maivspapaw rtilpcmlgv psigddatwv twkmaltiam picaicvvvm viyhvymtkp
       61 ptppdpddsd rpildgvtir dmlekttsgs gsvglpllvq rsiarqiqlv etigkgrfge
      121 vwrgrwrgen vavkifssre erswfrevei yqtvmlrhdn ilgfiaadnk dngtwtqlwl
      181 itdyhekgsl fdylnvstvd tngmirmals ittglahlhm eivgtqgkpa iahrdlkskn
      241 ilvktngtca igdlglavrh dvrtntvdiq lnnnrvgtkr ymapevleet vnmnhfesfk
      301 radvyalgli lweiarrcnv ggihdeyqlp fydlvpsdpt ieemrkvvct drqrpsipnr
      361 wqsiealqvm skvmkecwyh naaarltalr ikkslanyga medlk
//
LOCUS       XP_034195581             479 aa            linear   INV 03-SEP-2025
DEFINITION  UDP-glucose 6-dehydrogenase sgl [Osmia lignaria lignaria].
ACCESSION   XP_034195581
VERSION     XP_034195581.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339690.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="UDP-glucose 6-dehydrogenase sgl"
                     /calculated_mol_wt=52870
     Region          3..462
                     /region_name="PLN02353"
                     /note="probable UDP-glucose 6-dehydrogenase"
                     /db_xref="CDD:177986"
     CDS             1..479
                     /gene="sgl"
                     /coded_by="XM_034339690.2:370..1809"
                     /db_xref="GeneID:117611654"
ORIGIN      
        1 mtikkicgig agyvggptcs vialkcpeiq vtvvdksker iaqwnseklp iyepglddvv
       61 rkcrgknlyf stnietaiqe adlifisvnt ptktfgngkg raadlkyves aarmiaeiat
      121 gdkivvekst vpvraaesim nilranhkpg vsyqilsnpe flaegtaikd lvnaervlig
      181 gedspegqaa ieelskvyeh wiprkniltt ntwsselskl aanaflaqri ssinslsavc
      241 eatgadvsev aravgldsri gskflhasvg fggscfqkdi lnlvyicecl nlpevaaywq
      301 qvidmneyqk srfsakvies lfntvtdkki smlgfafkkn tgdtrespai hvaktlldeg
      361 amlhiydpkv eetqimedlm hpsitsnpqh vknrisiykd aysatrgtha ivvctewdef
      421 ieldyeqiyl smmkpayifd grkildhdsl qkigfivqti gkritrtvls rawgsqtqi
//
LOCUS       XP_034195582             292 aa            linear   INV 03-SEP-2025
DEFINITION  paramyosin, short form isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195582
VERSION     XP_034195582.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339691.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195582.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="paramyosin, short form isoform X1"
                     /calculated_mol_wt=34393
     CDS             1..292
                     /gene="LOC117611655"
                     /coded_by="XM_034339691.2:767..1645"
                     /db_xref="GeneID:117611655"
ORIGIN      
        1 magylppmyv psepkwkhwp tyiydknysc gvnyyqpmld yierqgrsny rssrmseppe
       61 lpwsdgralw edkpvepysr helirraida edeardhlsh fkianrsdfs lsktaqashv
      121 trevfprkle evrlrslpll vqsavvrnra rqiqrdmani efqslkdiqn msevqdaldh
      181 grslrgksak aiefhlraea lknltrseel adirkyqren msvlwdgrdh lrlmeqrtrn
      241 lidedkltap lnnlsrelrg yeekssnyfl nkryqrllrp arlygclgir pa
//
LOCUS       XP_034195583             287 aa            linear   INV 03-SEP-2025
DEFINITION  paramyosin, short form isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195583
VERSION     XP_034195583.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339692.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195583.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="paramyosin, short form isoform X2"
                     /calculated_mol_wt=34064
     CDS             1..287
                     /gene="LOC117611655"
                     /coded_by="XM_034339692.2:767..1630"
                     /db_xref="GeneID:117611655"
ORIGIN      
        1 magylppmyv psepkwkhwp tyiydknysc gvnyyqpmld yierqgrsny rssrmseppe
       61 lpwsdgralw edkpvepysr helirraida edeardhlsh fkianrsdfs lsktaqashv
      121 trevfprkle evrlrslpll vqsavvrnra rqiqrdmani efqslkdiqn msevqdaldh
      181 grslrgksak aiefhlraea lknltrseel adirkyqren msvlwdgrdh lrlmeqrtrn
      241 lidedkltap lnnlsrelrg yeekssnyfl nkrfyrrssv rrwnkec
//
LOCUS       XP_034195584             269 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L7A [Osmia lignaria lignaria].
ACCESSION   XP_034195584
VERSION     XP_034195584.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339693.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="ribosomal protein L7A"
                     /calculated_mol_wt=30401
     Region          18..269
                     /region_name="SNU13"
                     /note="U4/U6.U5 small nuclear ribonucleoprotein SNU13;
                     cl42391"
                     /db_xref="CDD:455736"
     Site            order(144,147..148,151,166,168..169,171..172,175)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411046"
     CDS             1..269
                     /gene="RpL7A"
                     /coded_by="XM_034339693.2:134..943"
                     /db_xref="GeneID:117611657"
ORIGIN      
        1 mvqkkpkkkv gkkvaaapla vkkvepkkqt nplfekrprn fgigqdiqpa rdlsrfvkwp
       61 kyiriqrqra vlqkrlkvpp pinqftqald kqtatqlfkm lekyrpesti qkkmrlkaka
      121 eqrvakkeea ppakkpnvlr sgtntvttlv eqrkaqlvvi ahdvdpieiv lflptlcrkm
      181 gvpycivksk arlgrlvrrk tctavaltqv dsgdranfsk lveaiktnfn drydeirrhw
      241 gggllgsksa ariaklekak akelaqkqg
//
LOCUS       XP_034195521            1632 aa            linear   INV 03-SEP-2025
DEFINITION  cilia- and flagella-associated protein 43 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195521
VERSION     XP_034195521.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339630.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195521.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1632
                     /product="cilia- and flagella-associated protein 43"
                     /calculated_mol_wt=190645
     Region          1012..>1594
                     /region_name="CCDC158"
                     /note="Coiled-coil domain-containing protein 158;
                     pfam15921"
                     /db_xref="CDD:464943"
     CDS             1..1632
                     /gene="LOC117611619"
                     /coded_by="XM_034339630.2:83..4981"
                     /db_xref="GeneID:117611619"
ORIGIN      
        1 mllnndawsp swirgsklde ivwiakyvla wctginivff ninekklsfr wcwnsdsgeg
       61 arclsghskf pifafaektm qpriivisyp smtkvsectg gcpsgylata ftaqdymvsv
      121 gfyphfsmiv wnwrtgekii svnthirdev gqilritqge ptviaqmgkt cgtmftweln
      181 iagnvavlkd hevqlpnhgk iawidwcpts sepllaitdt fghiflsnyd gsnvyrivls
      241 qrcgictdia lplvhwfrdg ivlrttfcqi rfytknvrkd rwqrtwyvts vtkpyivvih
      301 psnnnrffyh tfegylmqid ffeedenpri skyldygaih rfvdfvypwc hhlvvsddsk
      361 gllilecysg tpvstfdadi dgevacqvsh pdypmivlgt tqgeaiflsl vqpitptiva
      421 slklqrepld likfsysgrf liaaekasgn cycinlhrek aytvqtlirv qrsitdllvy
      481 eafrklrvlv lfvgvaqyev gqqlmvfevq enqnlvtdvv eildlpgvyr aisyvpgnpt
      541 lfvgspyltr qlrvqkvqdy kdvimvdgla tghqvrlani fvdrawistt aldglvlvrd
      601 ktvrqlqtci mthhrtdfgt vkamvnrngd fivcigyngs lvavrsvpad nkkntatpgd
      661 epfmykvdya lyekyqrkif sdyvtldpta ygiltrrrsv fpgpkqkdkt wsewreefqi
      721 lqetetckee kekilqdfka lkgkvkklvd enevcpkiek lpmssfdldk agrdhklkag
      781 rddcedlrlk lehdigemkr vtrwiretfw npqtilgksi iaifgtehvt nypsvaedsn
      841 vkyrlqlatf ykeviqtive netfrpwqly dkeeleielg krlklkqeer rridllfaee
      901 edeeiveedt keeiamegtt thrfiaaspy ysqlecyafd qllinnyflm hdcaelrihf
      961 nrafdemyek keremnmiqa rierirhids elrsmfgqhv prvptdskwh wkerpediik
     1021 vldhevkakp yvspsqqeil dkqaaeeeri rqllladdfr eralmtmmgg vlevrwedti
     1081 kidvpkpacm lqkkpedyta edlqvvkqye kdvqflqeer erykrmlete yvrvmgllqe
     1141 gidkfnekln elfklkmnie aainqmnlry irgyiqiynr eqslqeemkl ktritekeey
     1201 entlksdlqt yqnvhqqlia kyelldhkqk vmskrfksef ssmsklnlel lerqykrrpk
     1261 tnlknlgpse llelghhvlk ksrpiflpse cneylkaldh ldvrpttlpp tidtsqwdhl
     1321 irlrrlkida efktrskqvq ivdaetvilg feqriekcka evertkknle etrrrrtisn
     1381 ldvevqlvlk mgqveintng nmkdtetail ishneiesvn kliraagack lkalsqllnf
     1441 qqgtlmkqwe heckrkrled lredlrftes vtvtkemqlf lkrkargmke dktqqqlaeh
     1501 ieamkqgfeq nldeqkdrlq siqteiestr sknkqldqqi lemnvarcem eqrrdligea
     1561 rqqehaekkl kmimkraali kklqdnyael lelqtehell rlrryptfgf kmlddkpkpn
     1621 ddppsrfdsc pc
//
LOCUS       XP_034195553            4802 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195553
VERSION     XP_034195553.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339662.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195553.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4802
                     /product="FAT atypical cadherin kugelei isoform X1"
                     /calculated_mol_wt=532428
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1370..1456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1472..1561
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1477..1478,1527,1529,1557,1559..1560)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1569..1667
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1576..1577,1630,1632,1663,1665..1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1675..1767
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1780..1870
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1787..1788,1835,1837,1866,1868..1869)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1878..1983
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1885..1886,1945,1947,1980,1982..1983)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <2014..2079
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2092..2189
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2099..2100,2149,2151,2185,2187..2188)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2197..2288
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2299..2399
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2305..2306,2364,2366,2396,2398..2399)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2408..2507
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2415..2416,2470,2472,2503,2505..2506)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2515..2608
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2522..2523,2574,2576,2604,2606..2607)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2618..2711
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2623..2624,2676,2678,2707,2709..2710)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2719..2818
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2726..2727,2782,2784,2815,2817..2818)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2830..2919
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2834..2835,2882,2884,2915,2917..2918)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2927..3026
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2934..2935,2989,2991,3022,3024..3025)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3035..3127
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3041..3042,3093,3095,3123,3125..3126)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3136..3231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3142..3143,3194,3196,3227,3229..3230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3239..3335
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3246..3247,3298,3300,3331,3333..3334)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3343..3440
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3350..3351,3403,3405,3436,3438..3439)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3448..3545
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3455..3456,3508,3510,3541,3543..3544)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3554..3645
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3669..3743
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3926..3962
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3980..4136
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4176..4205
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4211..4250
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4211,4214,4230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4255..4284
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4288..4323
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4288,4291,4305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4802
                     /gene="kug"
                     /coded_by="XM_034339662.2:503..14911"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqksrnrs refdlsidtl lengtwetfs pdslsgdrif
     1381 rvlandkdig dngkiqysik ggrgkgkfki hpttgmvysq rgfeagqeye mmiraadsge
     1441 pqrshqtrvs vqvvevpkes enppvfktnn qrvevtesde vgflvalmqa tdkdgdslwy
     1501 dildgdkrde ffigrdngnv llakkldwet qnfynltigv tdsvhtavtq llvtvidind
     1561 hrpeftetty rvdisenvek gemilqlhat dededkkvfy slhaaqnqas leifhvdsvt
     1621 gsvtlnevld retieehvlt vmvkdqgtpa krnyarvivt vhdhndhape fiseiiqgkv
     1681 fetspigaav vqvwaidrdr gdnakityti tsgnvgnmfs idpelgvirv areldlsvss
     1741 eyillvkatd hgspalantv pvhvmvtmad napprfiqke lsaeiyenqq lgtyvkhvea
     1801 rstsslqfei vdgnkddtff vnpstgiivi kneldyektk fynltvaatn magakahcnv
     1861 ivhvldrndn aprflqatyt geisegasig slvltntstp lvikaedads elnallnydi
     1921 vedlprkyfh idsstgairt vmvldhesip kftfhvkvsd lgkpklsset takvmivvtd
     1981 vndcppkfle ndynttlllp tyknvavvrv navdpdsseg valrydiidg nkghtfdida
     2041 qtgvitvynp erikknyllr vrvsdgkyss vtqvnvavek sensglvfqk evyegsipen
     2101 strittvavv nvlgsalneh ivfsilnptd mfvigptsga irttgirfdr evcdhyeliv
     2161 eaksqmpdre kprvahvivn vtildindnc pmfvnlpyya vvsvdaqkgd vitkvhaidm
     2221 dsgdngevry elkkghgelf kvcrktgeis lkqnleghnr eyqltiaayd ggntpcsiev
     2281 pvnvkvidrs mpvfdkqfyt dsvlesieih splalsiqae splnrkliys itkgndfeef
     2341 aldfntapds nngpcviyvv deldyeqkkq yeltvratds vsgvyaevlv silvldvndc
     2401 ppeftqdsyn isvseaapfg tsvlkvssrd ndtginqqvr yaiqndtens selfhidpee
     2461 gviflkrsld hesheshhft viaidrgvps lsstahvwvt vidmndnppk feqpsytcsl
     2521 sehaergqfv tvvsasdpdy vdekltytiv ggneqqtyni dpstgiisli nmqnfgekkl
     2581 tmlnvsvtdn vytsfarvki eilpanrhnp kfpnpvvevt vlenqlpgrl vtsviasded
     2641 fgeygtviyt iasdlmkeif dinrltgeiv trkkldreeq klyeipvmat dgggrsgfvm
     2701 vrvkvgdend nspvfllrey kasihgnltv ntpflkvkaq dadeddaari vysiydlqss
     2761 eakalfginp dtgalflqks aipwenqlfe lfiraedkga mthhadvpls vyimgpqdip
     2821 plferkedkf fiseasaigt pitrlrtvtn ttvtyrivsg dedsphfmvd shgqvtlikp
     2881 ldretkdnhl igvlaetdss plltalaeis lqvldendha pefesnpygi ilaenieegt
     2941 silkiiahdd dlgsngevry sfgsdigela nvftidaytg wistlvqldk ekqpeykfqv
     3001 vatdngnpkh fartsvhvkl kdyndnppaf vddryeatvn edalpgtvvv klitvdkdsd
     3061 vntpiefyit sgdsrsqfqi rstgevyvak aldretidry eleivgtdgk yvfktrvtvq
     3121 vldvndnppy clryryreil segshpgtyv ltvlatdydd epnaklrfyl tgdnndkfsl
     3181 dketgvlktv gqldretqak ysltahvqdr dkpswecssq leilisdlnd nppkftmqty
     3241 satlpedvev gtlvtkvhat dddiginrki ryefidsadg qfliaadsgi vtlgksldre
     3301 tkamynvtiq aldqgtpqlt svtslivnvq dindnppefa skvyfskvpe iyavgtevar
     3361 vlatskdtgv nadvyysivg gnehkkfqid arsgvitiae qldyerardy fltiqavdgg
     3421 ipplsnhatv nitvidsndn apifsevsyr asvredakig ekvtqvfand ldseengnvs
     3481 yyiergdrqk qfsidqktgq itvaapldre eianyvlevh grdsgipmls sfvmvnievl
     3541 dandnpplfs lsnytavvqe dkplghtvlq fvvtdadiep nadpytfdfr sgnegdafrl
     3601 eqdgtlrtat kfnnrvkdky vlhirvfdng spplysdawv tikvieesqy ppvitplevi
     3661 insymdeypg giigkvhatd qdqydtlmys lvssspipnd lfkidkidgt lvalprldvg
     3721 eyrvnvtvtd gkfhsyslvk vnvdlitekm lensvivrfr evspedfmls hrkgfvrtlr
     3781 namncrpkdi viisvqpssd eanliksrai rqavhndldl lfavkksgta lasptfyvse
     3841 sirealnqri eeleestklv veeivrfkcn kgycvygdcq dkivldltqi tpvatdvmsf
     3901 vsprhkhkme cnckegyagn hcemvvneca rdpcplfkvc vpdssvqgys cqcpegfagq
     3961 tcdidiskch descyiprnp isfsgksyar yridkalirq tiedrlslsl rirtvqltgn
     4021 lmyaagkvdy nilevvngvv qykfdlgsge glvrvssvyv sdgqwheiql eresnsarlt
     4081 vdgkhvahgs apgindilnl qsddlylgae vrqhpsilgf edvqrgfigc mddvriarvs
     4141 vplhmsgass vavlkrfanv efscdattvl vppgicgsqp cqnggtckds ggdtyecqch
     4201 srfaglacei dtdpcasspc lyggrckivp eggdyscecv gpslsgkrce fgrycspnpc
     4261 ihggvceegn ngpickcqgf mgdrcetdvn ecdtnpctng gtcvneigty rcvcppnmtg
     4321 lncgnpayst piisshfnit weqlmwigva vilnvfliii flafrrirmk rtrarannin
     4381 netrkqivln sarphehefk rssklsnlev iqreppqcpp rpasytpssn nepapysnsa
     4441 avalnnldtl rsygsagdel enfppdylrn lnrttpvppp sltlanpvgg natgsdtdsl
     4501 hkpswqeqmq lasfitdttk ikndlkrasp vvpelttggl llnssrrvph gggtsvasmt
     4561 sieddprivg gyhwdcsdwv rpsqnplpni tevpgsevpd sssfhsnesn esnthqlpsv
     4621 hgsvdptrdi etlnedqese yvgdsecgte fseqyqcaet qrlnpldsgg egeykykgse
     4681 sylrhpnsyl phynihtdte netnplngrl stlesdeeed dvvpygfpss rrnrchkgde
     4741 adeigsvitt leernsllgg atsnsdlstn lceiddseye iadqksngrl wlsgngitqt
     4801 sv
//
LOCUS       XP_034195554            4795 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195554
VERSION     XP_034195554.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339663.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195554.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4795
                     /product="FAT atypical cadherin kugelei isoform X2"
                     /calculated_mol_wt=531579
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1370..1456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1472..1561
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1477..1478,1527,1529,1557,1559..1560)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1569..1667
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1576..1577,1630,1632,1663,1665..1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1675..1767
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1780..1870
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1787..1788,1835,1837,1866,1868..1869)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1878..1983
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1885..1886,1945,1947,1980,1982..1983)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <2014..2079
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2092..2189
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2099..2100,2149,2151,2185,2187..2188)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2197..2288
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2299..2399
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2305..2306,2364,2366,2396,2398..2399)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2408..2507
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2415..2416,2470,2472,2503,2505..2506)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2515..2608
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2522..2523,2574,2576,2604,2606..2607)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2618..2711
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2623..2624,2676,2678,2707,2709..2710)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2719..2818
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2726..2727,2782,2784,2815,2817..2818)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2830..2919
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2834..2835,2882,2884,2915,2917..2918)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2927..3026
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2934..2935,2989,2991,3022,3024..3025)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3035..3127
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3041..3042,3093,3095,3123,3125..3126)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3136..3231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3142..3143,3194,3196,3227,3229..3230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3239..3335
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3246..3247,3298,3300,3331,3333..3334)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3343..3440
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3350..3351,3403,3405,3436,3438..3439)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3448..3545
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3455..3456,3508,3510,3541,3543..3544)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3554..3645
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3669..3743
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3926..3962
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3980..4136
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4176..4205
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4211..4250
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4211,4214,4230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4255..4284
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4288..4323
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4288,4291,4305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4795
                     /gene="kug"
                     /coded_by="XM_034339663.2:503..14890"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqksrnrs refdlsidtl lengtwetfs pdslsgdrif
     1381 rvlandkdig dngkiqysik ggrgkgkfki hpttgmvysq rgfeagqeye mmiraadsge
     1441 pqrshqtrvs vqvvevpkes enppvfktnn qrvevtesde vgflvalmqa tdkdgdslwy
     1501 dildgdkrde ffigrdngnv llakkldwet qnfynltigv tdsvhtavtq llvtvidind
     1561 hrpeftetty rvdisenvek gemilqlhat dededkkvfy slhaaqnqas leifhvdsvt
     1621 gsvtlnevld retieehvlt vmvkdqgtpa krnyarvivt vhdhndhape fiseiiqgkv
     1681 fetspigaav vqvwaidrdr gdnakityti tsgnvgnmfs idpelgvirv areldlsvss
     1741 eyillvkatd hgspalantv pvhvmvtmad napprfiqke lsaeiyenqq lgtyvkhvea
     1801 rstsslqfei vdgnkddtff vnpstgiivi kneldyektk fynltvaatn magakahcnv
     1861 ivhvldrndn aprflqatyt geisegasig slvltntstp lvikaedads elnallnydi
     1921 vedlprkyfh idsstgairt vmvldhesip kftfhvkvsd lgkpklsset takvmivvtd
     1981 vndcppkfle ndynttlllp tyknvavvrv navdpdsseg valrydiidg nkghtfdida
     2041 qtgvitvynp erikknyllr vrvsdgkyss vtqvnvavek sensglvfqk evyegsipen
     2101 strittvavv nvlgsalneh ivfsilnptd mfvigptsga irttgirfdr evcdhyeliv
     2161 eaksqmpdre kprvahvivn vtildindnc pmfvnlpyya vvsvdaqkgd vitkvhaidm
     2221 dsgdngevry elkkghgelf kvcrktgeis lkqnleghnr eyqltiaayd ggntpcsiev
     2281 pvnvkvidrs mpvfdkqfyt dsvlesieih splalsiqae splnrkliys itkgndfeef
     2341 aldfntapds nngpcviyvv deldyeqkkq yeltvratds vsgvyaevlv silvldvndc
     2401 ppeftqdsyn isvseaapfg tsvlkvssrd ndtginqqvr yaiqndtens selfhidpee
     2461 gviflkrsld hesheshhft viaidrgvps lsstahvwvt vidmndnppk feqpsytcsl
     2521 sehaergqfv tvvsasdpdy vdekltytiv ggneqqtyni dpstgiisli nmqnfgekkl
     2581 tmlnvsvtdn vytsfarvki eilpanrhnp kfpnpvvevt vlenqlpgrl vtsviasded
     2641 fgeygtviyt iasdlmkeif dinrltgeiv trkkldreeq klyeipvmat dgggrsgfvm
     2701 vrvkvgdend nspvfllrey kasihgnltv ntpflkvkaq dadeddaari vysiydlqss
     2761 eakalfginp dtgalflqks aipwenqlfe lfiraedkga mthhadvpls vyimgpqdip
     2821 plferkedkf fiseasaigt pitrlrtvtn ttvtyrivsg dedsphfmvd shgqvtlikp
     2881 ldretkdnhl igvlaetdss plltalaeis lqvldendha pefesnpygi ilaenieegt
     2941 silkiiahdd dlgsngevry sfgsdigela nvftidaytg wistlvqldk ekqpeykfqv
     3001 vatdngnpkh fartsvhvkl kdyndnppaf vddryeatvn edalpgtvvv klitvdkdsd
     3061 vntpiefyit sgdsrsqfqi rstgevyvak aldretidry eleivgtdgk yvfktrvtvq
     3121 vldvndnppy clryryreil segshpgtyv ltvlatdydd epnaklrfyl tgdnndkfsl
     3181 dketgvlktv gqldretqak ysltahvqdr dkpswecssq leilisdlnd nppkftmqty
     3241 satlpedvev gtlvtkvhat dddiginrki ryefidsadg qfliaadsgi vtlgksldre
     3301 tkamynvtiq aldqgtpqlt svtslivnvq dindnppefa skvyfskvpe iyavgtevar
     3361 vlatskdtgv nadvyysivg gnehkkfqid arsgvitiae qldyerardy fltiqavdgg
     3421 ipplsnhatv nitvidsndn apifsevsyr asvredakig ekvtqvfand ldseengnvs
     3481 yyiergdrqk qfsidqktgq itvaapldre eianyvlevh grdsgipmls sfvmvnievl
     3541 dandnpplfs lsnytavvqe dkplghtvlq fvvtdadiep nadpytfdfr sgnegdafrl
     3601 eqdgtlrtat kfnnrvkdky vlhirvfdng spplysdawv tikvieesqy ppvitplevi
     3661 insymdeypg giigkvhatd qdqydtlmys lvssspipnd lfkidkidgt lvalprldvg
     3721 eyrvnvtvtd gkfhsyslvk vnvdlitekm lensvivrfr evspedfmls hrkgfvrtlr
     3781 namncrpkdi viisvqpssd eanliksrai rqavhndldl lfavkksgta lasptfyvse
     3841 sirealnqri eeleestklv veeivrfkcn kgycvygdcq dkivldltqi tpvatdvmsf
     3901 vsprhkhkme cnckegyagn hcemvvneca rdpcplfkvc vpdssvqgys cqcpegfagq
     3961 tcdidiskch descyiprnp isfsgksyar yridkalirq tiedrlslsl rirtvqltgn
     4021 lmyaagkvdy nilevvngvv qykfdlgsge glvrvssvyv sdgqwheiql eresnsarlt
     4081 vdgkhvahgs apgindilnl qsddlylgae vrqhpsilgf edvqrgfigc mddvriarvs
     4141 vplhmsgass vavlkrfanv efscdattvl vppgicgsqp cqnggtckds ggdtyecqch
     4201 srfaglacei dtdpcasspc lyggrckivp eggdyscecv gpslsgkrce fgrycspnpc
     4261 ihggvceegn ngpickcqgf mgdrcetdvn ecdtnpctng gtcvneigty rcvcppnmtg
     4321 lncgnpayst piisshfnit weqlmwigva vilnvfliii flafrrirmk rtrarannin
     4381 netrkqivln sarphehefk rssklsnlev iqreppqcpp rpasytpssn nepapysnsa
     4441 avalnnldtl rsygsagdel enfppdylrn lnrttpvppp sltlanpvgg natgsdtdsl
     4501 hkpswqeqmq lasfitdttk ikndlkrasp vvpelttggl llnssrrvph gggtsvasmt
     4561 sieddprivg dwvrpsqnpl pnitevpgse vpdsssfhsn esnesnthql psvhgsvdpt
     4621 rdietlnedq eseyvgdsec gtefseqyqc aetqrlnpld sggegeykyk gsesylrhpn
     4681 sylphyniht dtenetnpln grlstlesde eeddvvpygf pssrrnrchk gdeadeigsv
     4741 ittleernsl lggatsnsdl stnlceidds eyeiadqksn grlwlsgngi tqtsv
//
LOCUS       XP_034195555            4794 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195555
VERSION     XP_034195555.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339664.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195555.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4794
                     /product="FAT atypical cadherin kugelei isoform X3"
                     /calculated_mol_wt=531629
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1370..1456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1472..1561
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1477..1478,1527,1529,1557,1559..1560)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1569..1667
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1576..1577,1630,1632,1663,1665..1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1675..1767
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1780..1870
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1787..1788,1835,1837,1866,1868..1869)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1878..1983
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1885..1886,1945,1947,1980,1982..1983)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <2014..2079
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2092..2189
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2099..2100,2149,2151,2185,2187..2188)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2197..2288
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2299..2391
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2305..2306,2356,2358,2388,2390..2391)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2400..2499
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2407..2408,2462,2464,2495,2497..2498)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2507..2600
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2514..2515,2566,2568,2596,2598..2599)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2610..2703
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2615..2616,2668,2670,2699,2701..2702)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2711..2810
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2718..2719,2774,2776,2807,2809..2810)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2822..2911
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2826..2827,2874,2876,2907,2909..2910)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2919..3018
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2926..2927,2981,2983,3014,3016..3017)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3027..3119
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3033..3034,3085,3087,3115,3117..3118)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3128..3223
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3134..3135,3186,3188,3219,3221..3222)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3231..3327
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3238..3239,3290,3292,3323,3325..3326)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3335..3432
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3342..3343,3395,3397,3428,3430..3431)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3440..3537
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3447..3448,3500,3502,3533,3535..3536)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3546..3637
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3661..3735
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3918..3954
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3972..4128
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4168..4197
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4203..4242
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4203,4206,4222)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4247..4276
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4280..4315
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4280,4283,4297)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4794
                     /gene="kug"
                     /coded_by="XM_034339664.2:503..14887"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqksrnrs refdlsidtl lengtwetfs pdslsgdrif
     1381 rvlandkdig dngkiqysik ggrgkgkfki hpttgmvysq rgfeagqeye mmiraadsge
     1441 pqrshqtrvs vqvvevpkes enppvfktnn qrvevtesde vgflvalmqa tdkdgdslwy
     1501 dildgdkrde ffigrdngnv llakkldwet qnfynltigv tdsvhtavtq llvtvidind
     1561 hrpeftetty rvdisenvek gemilqlhat dededkkvfy slhaaqnqas leifhvdsvt
     1621 gsvtlnevld retieehvlt vmvkdqgtpa krnyarvivt vhdhndhape fiseiiqgkv
     1681 fetspigaav vqvwaidrdr gdnakityti tsgnvgnmfs idpelgvirv areldlsvss
     1741 eyillvkatd hgspalantv pvhvmvtmad napprfiqke lsaeiyenqq lgtyvkhvea
     1801 rstsslqfei vdgnkddtff vnpstgiivi kneldyektk fynltvaatn magakahcnv
     1861 ivhvldrndn aprflqatyt geisegasig slvltntstp lvikaedads elnallnydi
     1921 vedlprkyfh idsstgairt vmvldhesip kftfhvkvsd lgkpklsset takvmivvtd
     1981 vndcppkfle ndynttlllp tyknvavvrv navdpdsseg valrydiidg nkghtfdida
     2041 qtgvitvynp erikknyllr vrvsdgkyss vtqvnvavek sensglvfqk evyegsipen
     2101 strittvavv nvlgsalneh ivfsilnptd mfvigptsga irttgirfdr evcdhyeliv
     2161 eaksqmpdre kprvahvivn vtildindnc pmfvnlpyya vvsvdaqkgd vitkvhaidm
     2221 dsgdngevry elkkghgelf kvcrktgeis lkqnleghnr eyqltiaayd ggntpcsiev
     2281 pvnvkvidrs mpvfdkqfyt dsvlesieih splalsiqae splnrkliys itkgndfeef
     2341 aldfntgviy vvdeldyeqk kqyeltvrat dsvsgvyaev lvsilvldvn dcppeftqds
     2401 ynisvseaap fgtsvlkvss rdndtginqq vryaiqndte nsselfhidp eegviflkrs
     2461 ldhesheshh ftviaidrgv pslsstahvw vtvidmndnp pkfeqpsytc slsehaergq
     2521 fvtvvsasdp dyvdekltyt ivggneqqty nidpstgiis linmqnfgek kltmlnvsvt
     2581 dnvytsfarv kieilpanrh npkfpnpvve vtvlenqlpg rlvtsviasd edfgeygtvi
     2641 ytiasdlmke ifdinrltge ivtrkkldre eqklyeipvm atdgggrsgf vmvrvkvgde
     2701 ndnspvfllr eykasihgnl tvntpflkvk aqdadeddaa rivysiydlq sseakalfgi
     2761 npdtgalflq ksaipwenql felfiraedk gamthhadvp lsvyimgpqd ipplferked
     2821 kffiseasai gtpitrlrtv tnttvtyriv sgdedsphfm vdshgqvtli kpldretkdn
     2881 hligvlaetd ssplltalae islqvldend hapefesnpy giilaeniee gtsilkiiah
     2941 dddlgsngev rysfgsdige lanvftiday tgwistlvql dkekqpeykf qvvatdngnp
     3001 khfartsvhv klkdyndnpp afvddryeat vnedalpgtv vvklitvdkd sdvntpiefy
     3061 itsgdsrsqf qirstgevyv akaldretid ryeleivgtd gkyvfktrvt vqvldvndnp
     3121 pyclryryre ilsegshpgt yvltvlatdy ddepnaklrf yltgdnndkf sldketgvlk
     3181 tvgqldretq akysltahvq drdkpswecs sqleilisdl ndnppkftmq tysatlpedv
     3241 evgtlvtkvh atdddiginr kiryefidsa dgqfliaads givtlgksld retkamynvt
     3301 iqaldqgtpq ltsvtslivn vqdindnppe faskvyfskv peiyavgtev arvlatskdt
     3361 gvnadvyysi vggnehkkfq idarsgviti aeqldyerar dyfltiqavd ggipplsnha
     3421 tvnitvidsn dnapifsevs yrasvredak igekvtqvfa ndldseengn vsyyiergdr
     3481 qkqfsidqkt gqitvaapld reeianyvle vhgrdsgipm lssfvmvnie vldandnppl
     3541 fslsnytavv qedkplghtv lqfvvtdadi epnadpytfd frsgnegdaf rleqdgtlrt
     3601 atkfnnrvkd kyvlhirvfd ngspplysda wvtikviees qyppvitple viinsymdey
     3661 pggiigkvha tdqdqydtlm yslvssspip ndlfkidkid gtlvalprld vgeyrvnvtv
     3721 tdgkfhsysl vkvnvdlite kmlensvivr frevspedfm lshrkgfvrt lrnamncrpk
     3781 diviisvqps sdeanliksr airqavhndl dllfavkksg talasptfyv sesirealnq
     3841 rieeleestk lvveeivrfk cnkgycvygd cqdkivldlt qitpvatdvm sfvsprhkhk
     3901 mecnckegya gnhcemvvne cardpcplfk vcvpdssvqg yscqcpegfa gqtcdidisk
     3961 chdescyipr npisfsgksy aryridkali rqtiedrlsl slrirtvqlt gnlmyaagkv
     4021 dynilevvng vvqykfdlgs geglvrvssv yvsdgqwhei qleresnsar ltvdgkhvah
     4081 gsapgindil nlqsddlylg aevrqhpsil gfedvqrgfi gcmddvriar vsvplhmsga
     4141 ssvavlkrfa nvefscdatt vlvppgicgs qpcqnggtck dsggdtyecq chsrfaglac
     4201 eidtdpcass pclyggrcki vpeggdysce cvgpslsgkr cefgrycspn pcihggvcee
     4261 gnngpickcq gfmgdrcetd vnecdtnpct nggtcvneig tyrcvcppnm tglncgnpay
     4321 stpiisshfn itweqlmwig vavilnvfli iiflafrrir mkrtrarann innetrkqiv
     4381 lnsarphehe fkrssklsnl eviqreppqc pprpasytps snnepapysn saavalnnld
     4441 tlrsygsagd elenfppdyl rnlnrttpvp ppsltlanpv ggnatgsdtd slhkpswqeq
     4501 mqlasfitdt tkikndlkra spvvpelttg glllnssrrv phgggtsvas mtsieddpri
     4561 vggyhwdcsd wvrpsqnplp nitevpgsev pdsssfhsne snesnthqlp svhgsvdptr
     4621 dietlnedqe seyvgdsecg tefseqyqca etqrlnplds ggegeykykg sesylrhpns
     4681 ylphynihtd tenetnplng rlstlesdee eddvvpygfp ssrrnrchkg deadeigsvi
     4741 ttleernsll ggatsnsdls tnlceiddse yeiadqksng rlwlsgngit qtsv
//
LOCUS       XP_034195556            4765 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195556
VERSION     XP_034195556.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339665.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195556.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4765
                     /product="FAT atypical cadherin kugelei isoform X4"
                     /calculated_mol_wt=528099
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1328..1419
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1435..1524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1440..1441,1490,1492,1520,1522..1523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1532..1630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1539..1540,1593,1595,1626,1628..1629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1638..1730
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1743..1833
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1750..1751,1798,1800,1829,1831..1832)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1841..1946
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1848..1849,1908,1910,1943,1945..1946)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <1977..2042
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2055..2152
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2062..2063,2112,2114,2148,2150..2151)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2160..2251
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2262..2362
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2268..2269,2327,2329,2359,2361..2362)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2371..2470
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2378..2379,2433,2435,2466,2468..2469)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2478..2571
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2485..2486,2537,2539,2567,2569..2570)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2581..2674
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2586..2587,2639,2641,2670,2672..2673)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2682..2781
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2689..2690,2745,2747,2778,2780..2781)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2793..2882
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2797..2798,2845,2847,2878,2880..2881)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2890..2989
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2897..2898,2952,2954,2985,2987..2988)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2998..3090
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3004..3005,3056,3058,3086,3088..3089)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3099..3194
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3105..3106,3157,3159,3190,3192..3193)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3202..3298
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3209..3210,3261,3263,3294,3296..3297)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3306..3403
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3313..3314,3366,3368,3399,3401..3402)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3411..3508
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3418..3419,3471,3473,3504,3506..3507)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3517..3608
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3632..3706
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3889..3925
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3943..4099
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4139..4168
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4174..4213
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4174,4177,4193)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4218..4247
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4251..4286
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4251,4254,4268)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4765
                     /gene="kug"
                     /coded_by="XM_034339665.2:503..14800"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqvlandk digdngkiqy sikggrgkgk fkihpttgmv
     1381 ysqrgfeagq eyemmiraad sgepqrshqt rvsvqvvevp kesenppvfk tnnqrvevte
     1441 sdevgflval mqatdkdgds lwydildgdk rdeffigrdn gnvllakkld wetqnfynlt
     1501 igvtdsvhta vtqllvtvid indhrpefte ttyrvdisen vekgemilql hatdededkk
     1561 vfyslhaaqn qasleifhvd svtgsvtlne vldretieeh vltvmvkdqg tpakrnyarv
     1621 ivtvhdhndh apefiseiiq gkvfetspig aavvqvwaid rdrgdnakit ytitsgnvgn
     1681 mfsidpelgv irvareldls vsseyillvk atdhgspala ntvpvhvmvt madnapprfi
     1741 qkelsaeiye nqqlgtyvkh vearstsslq feivdgnkdd tffvnpstgi ivikneldye
     1801 ktkfynltva atnmagakah cnvivhvldr ndnaprflqa tytgeisega sigslvltnt
     1861 stplvikaed adselnalln ydivedlprk yfhidsstga irtvmvldhe sipkftfhvk
     1921 vsdlgkpkls settakvmiv vtdvndcppk flendynttl llptyknvav vrvnavdpds
     1981 segvalrydi idgnkghtfd idaqtgvitv ynperikkny llrvrvsdgk yssvtqvnva
     2041 veksensglv fqkevyegsi penstrittv avvnvlgsal nehivfsiln ptdmfvigpt
     2101 sgairttgir fdrevcdhye liveaksqmp drekprvahv ivnvtildin dncpmfvnlp
     2161 yyavvsvdaq kgdvitkvha idmdsgdnge vryelkkghg elfkvcrktg eislkqnleg
     2221 hnreyqltia aydggntpcs ievpvnvkvi drsmpvfdkq fytdsvlesi eihsplalsi
     2281 qaesplnrkl iysitkgndf eefaldfnta pdsnngpcvi yvvdeldyeq kkqyeltvra
     2341 tdsvsgvyae vlvsilvldv ndcppeftqd synisvseaa pfgtsvlkvs srdndtginq
     2401 qvryaiqndt ensselfhid peegviflkr sldheshesh hftviaidrg vpslsstahv
     2461 wvtvidmndn ppkfeqpsyt cslsehaerg qfvtvvsasd pdyvdeklty tivggneqqt
     2521 ynidpstgii slinmqnfge kkltmlnvsv tdnvytsfar vkieilpanr hnpkfpnpvv
     2581 evtvlenqlp grlvtsvias dedfgeygtv iytiasdlmk eifdinrltg eivtrkkldr
     2641 eeqklyeipv matdgggrsg fvmvrvkvgd endnspvfll reykasihgn ltvntpflkv
     2701 kaqdadedda arivysiydl qsseakalfg inpdtgalfl qksaipwenq lfelfiraed
     2761 kgamthhadv plsvyimgpq dipplferke dkffiseasa igtpitrlrt vtnttvtyri
     2821 vsgdedsphf mvdshgqvtl ikpldretkd nhligvlaet dssplltala eislqvlden
     2881 dhapefesnp ygiilaenie egtsilkiia hdddlgsnge vrysfgsdig elanvftida
     2941 ytgwistlvq ldkekqpeyk fqvvatdngn pkhfartsvh vklkdyndnp pafvddryea
     3001 tvnedalpgt vvvklitvdk dsdvntpief yitsgdsrsq fqirstgevy vakaldreti
     3061 dryeleivgt dgkyvfktrv tvqvldvndn ppyclryryr eilsegshpg tyvltvlatd
     3121 yddepnaklr fyltgdnndk fsldketgvl ktvgqldret qakysltahv qdrdkpswec
     3181 ssqleilisd lndnppkftm qtysatlped vevgtlvtkv hatdddigin rkiryefids
     3241 adgqfliaad sgivtlgksl dretkamynv tiqaldqgtp qltsvtsliv nvqdindnpp
     3301 efaskvyfsk vpeiyavgte varvlatskd tgvnadvyys ivggnehkkf qidarsgvit
     3361 iaeqldyera rdyfltiqav dggipplsnh atvnitvids ndnapifsev syrasvreda
     3421 kigekvtqvf andldseeng nvsyyiergd rqkqfsidqk tgqitvaapl dreeianyvl
     3481 evhgrdsgip mlssfvmvni evldandnpp lfslsnytav vqedkplght vlqfvvtdad
     3541 iepnadpytf dfrsgnegda frleqdgtlr tatkfnnrvk dkyvlhirvf dngspplysd
     3601 awvtikviee sqyppvitpl eviinsymde ypggiigkvh atdqdqydtl myslvssspi
     3661 pndlfkidki dgtlvalprl dvgeyrvnvt vtdgkfhsys lvkvnvdlit ekmlensviv
     3721 rfrevspedf mlshrkgfvr tlrnamncrp kdiviisvqp ssdeanliks rairqavhnd
     3781 ldllfavkks gtalasptfy vsesirealn qrieeleest klvveeivrf kcnkgycvyg
     3841 dcqdkivldl tqitpvatdv msfvsprhkh kmecnckegy agnhcemvvn ecardpcplf
     3901 kvcvpdssvq gyscqcpegf agqtcdidis kchdescyip rnpisfsgks yaryridkal
     3961 irqtiedrls lslrirtvql tgnlmyaagk vdynilevvn gvvqykfdlg sgeglvrvss
     4021 vyvsdgqwhe iqleresnsa rltvdgkhva hgsapgindi lnlqsddlyl gaevrqhpsi
     4081 lgfedvqrgf igcmddvria rvsvplhmsg assvavlkrf anvefscdat tvlvppgicg
     4141 sqpcqnggtc kdsggdtyec qchsrfagla ceidtdpcas spclyggrck ivpeggdysc
     4201 ecvgpslsgk rcefgrycsp npcihggvce egnngpickc qgfmgdrcet dvnecdtnpc
     4261 tnggtcvnei gtyrcvcppn mtglncgnpa ystpiisshf nitweqlmwi gvavilnvfl
     4321 iiiflafrri rmkrtraran ninnetrkqi vlnsarpheh efkrssklsn leviqreppq
     4381 cpprpasytp ssnnepapys nsaavalnnl dtlrsygsag delenfppdy lrnlnrttpv
     4441 pppsltlanp vggnatgsdt dslhkpswqe qmqlasfitd ttkikndlkr aspvvpeltt
     4501 gglllnssrr vphgggtsva smtsieddpr ivggyhwdcs dwvrpsqnpl pnitevpgse
     4561 vpdsssfhsn esnesnthql psvhgsvdpt rdietlnedq eseyvgdsec gtefseqyqc
     4621 aetqrlnpld sggegeykyk gsesylrhpn sylphyniht dtenetnpln grlstlesde
     4681 eeddvvpygf pssrrnrchk gdeadeigsv ittleernsl lggatsnsdl stnlceidds
     4741 eyeiadqksn grlwlsgngi tqtsv
//
LOCUS       XP_034195557            4757 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034195557
VERSION     XP_034195557.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339666.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195557.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4757
                     /product="FAT atypical cadherin kugelei isoform X5"
                     /calculated_mol_wt=527301
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1328..1419
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1435..1524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1440..1441,1490,1492,1520,1522..1523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1532..1630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1539..1540,1593,1595,1626,1628..1629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1638..1730
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1743..1833
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1750..1751,1798,1800,1829,1831..1832)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1841..1946
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1848..1849,1908,1910,1943,1945..1946)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <1977..2042
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2055..2152
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2062..2063,2112,2114,2148,2150..2151)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2160..2251
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2262..2354
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2268..2269,2319,2321,2351,2353..2354)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2363..2462
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2370..2371,2425,2427,2458,2460..2461)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2470..2563
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2477..2478,2529,2531,2559,2561..2562)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2573..2666
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2578..2579,2631,2633,2662,2664..2665)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2674..2773
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2681..2682,2737,2739,2770,2772..2773)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2785..2874
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2789..2790,2837,2839,2870,2872..2873)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2882..2981
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2889..2890,2944,2946,2977,2979..2980)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2990..3082
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2996..2997,3048,3050,3078,3080..3081)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3091..3186
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3097..3098,3149,3151,3182,3184..3185)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3194..3290
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3201..3202,3253,3255,3286,3288..3289)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3298..3395
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3305..3306,3358,3360,3391,3393..3394)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3403..3500
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3410..3411,3463,3465,3496,3498..3499)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3509..3600
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3624..3698
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3881..3917
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3935..4091
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4131..4160
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4166..4205
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4166,4169,4185)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4210..4239
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4243..4278
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4243,4246,4260)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4757
                     /gene="kug"
                     /coded_by="XM_034339666.2:503..14776"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqvlandk digdngkiqy sikggrgkgk fkihpttgmv
     1381 ysqrgfeagq eyemmiraad sgepqrshqt rvsvqvvevp kesenppvfk tnnqrvevte
     1441 sdevgflval mqatdkdgds lwydildgdk rdeffigrdn gnvllakkld wetqnfynlt
     1501 igvtdsvhta vtqllvtvid indhrpefte ttyrvdisen vekgemilql hatdededkk
     1561 vfyslhaaqn qasleifhvd svtgsvtlne vldretieeh vltvmvkdqg tpakrnyarv
     1621 ivtvhdhndh apefiseiiq gkvfetspig aavvqvwaid rdrgdnakit ytitsgnvgn
     1681 mfsidpelgv irvareldls vsseyillvk atdhgspala ntvpvhvmvt madnapprfi
     1741 qkelsaeiye nqqlgtyvkh vearstsslq feivdgnkdd tffvnpstgi ivikneldye
     1801 ktkfynltva atnmagakah cnvivhvldr ndnaprflqa tytgeisega sigslvltnt
     1861 stplvikaed adselnalln ydivedlprk yfhidsstga irtvmvldhe sipkftfhvk
     1921 vsdlgkpkls settakvmiv vtdvndcppk flendynttl llptyknvav vrvnavdpds
     1981 segvalrydi idgnkghtfd idaqtgvitv ynperikkny llrvrvsdgk yssvtqvnva
     2041 veksensglv fqkevyegsi penstrittv avvnvlgsal nehivfsiln ptdmfvigpt
     2101 sgairttgir fdrevcdhye liveaksqmp drekprvahv ivnvtildin dncpmfvnlp
     2161 yyavvsvdaq kgdvitkvha idmdsgdnge vryelkkghg elfkvcrktg eislkqnleg
     2221 hnreyqltia aydggntpcs ievpvnvkvi drsmpvfdkq fytdsvlesi eihsplalsi
     2281 qaesplnrkl iysitkgndf eefaldfntg viyvvdeldy eqkkqyeltv ratdsvsgvy
     2341 aevlvsilvl dvndcppeft qdsynisvse aapfgtsvlk vssrdndtgi nqqvryaiqn
     2401 dtensselfh idpeegvifl krsldheshe shhftviaid rgvpslssta hvwvtvidmn
     2461 dnppkfeqps ytcslsehae rgqfvtvvsa sdpdyvdekl tytivggneq qtynidpstg
     2521 iislinmqnf gekkltmlnv svtdnvytsf arvkieilpa nrhnpkfpnp vvevtvlenq
     2581 lpgrlvtsvi asdedfgeyg tviytiasdl mkeifdinrl tgeivtrkkl dreeqklyei
     2641 pvmatdgggr sgfvmvrvkv gdendnspvf llreykasih gnltvntpfl kvkaqdaded
     2701 daarivysiy dlqsseakal fginpdtgal flqksaipwe nqlfelfira edkgamthha
     2761 dvplsvyimg pqdipplfer kedkffisea saigtpitrl rtvtnttvty rivsgdedsp
     2821 hfmvdshgqv tlikpldret kdnhligvla etdsspllta laeislqvld endhapefes
     2881 npygiilaen ieegtsilki iahdddlgsn gevrysfgsd igelanvfti daytgwistl
     2941 vqldkekqpe ykfqvvatdn gnpkhfarts vhvklkdynd nppafvddry eatvnedalp
     3001 gtvvvklitv dkdsdvntpi efyitsgdsr sqfqirstge vyvakaldre tidryeleiv
     3061 gtdgkyvfkt rvtvqvldvn dnppyclryr yreilsegsh pgtyvltvla tdyddepnak
     3121 lrfyltgdnn dkfsldketg vlktvgqldr etqakyslta hvqdrdkpsw ecssqleili
     3181 sdlndnppkf tmqtysatlp edvevgtlvt kvhatdddig inrkiryefi dsadgqflia
     3241 adsgivtlgk sldretkamy nvtiqaldqg tpqltsvtsl ivnvqdindn ppefaskvyf
     3301 skvpeiyavg tevarvlats kdtgvnadvy ysivggnehk kfqidarsgv itiaeqldye
     3361 rardyfltiq avdggippls nhatvnitvi dsndnapifs evsyrasvre dakigekvtq
     3421 vfandldsee ngnvsyyier gdrqkqfsid qktgqitvaa pldreeiany vlevhgrdsg
     3481 ipmlssfvmv nievldandn pplfslsnyt avvqedkplg htvlqfvvtd adiepnadpy
     3541 tfdfrsgneg dafrleqdgt lrtatkfnnr vkdkyvlhir vfdngspply sdawvtikvi
     3601 eesqyppvit pleviinsym deypggiigk vhatdqdqyd tlmyslvsss pipndlfkid
     3661 kidgtlvalp rldvgeyrvn vtvtdgkfhs yslvkvnvdl itekmlensv ivrfrevspe
     3721 dfmlshrkgf vrtlrnamnc rpkdiviisv qpssdeanli ksrairqavh ndldllfavk
     3781 ksgtalaspt fyvsesirea lnqrieelee stklvveeiv rfkcnkgycv ygdcqdkivl
     3841 dltqitpvat dvmsfvsprh khkmecncke gyagnhcemv vnecardpcp lfkvcvpdss
     3901 vqgyscqcpe gfagqtcdid iskchdescy iprnpisfsg ksyaryridk alirqtiedr
     3961 lslslrirtv qltgnlmyaa gkvdynilev vngvvqykfd lgsgeglvrv ssvyvsdgqw
     4021 heiqleresn sarltvdgkh vahgsapgin dilnlqsddl ylgaevrqhp silgfedvqr
     4081 gfigcmddvr iarvsvplhm sgassvavlk rfanvefscd attvlvppgi cgsqpcqngg
     4141 tckdsggdty ecqchsrfag laceidtdpc asspclyggr ckivpeggdy scecvgpsls
     4201 gkrcefgryc spnpcihggv ceegnngpic kcqgfmgdrc etdvnecdtn pctnggtcvn
     4261 eigtyrcvcp pnmtglncgn paystpiiss hfnitweqlm wigvavilnv fliiiflafr
     4321 rirmkrtrar anninnetrk qivlnsarph ehefkrsskl snleviqrep pqcpprpasy
     4381 tpssnnepap ysnsaavaln nldtlrsygs agdelenfpp dylrnlnrtt pvpppsltla
     4441 npvggnatgs dtdslhkpsw qeqmqlasfi tdttkikndl kraspvvpel ttgglllnss
     4501 rrvphgggts vasmtsiedd privggyhwd csdwvrpsqn plpnitevpg sevpdsssfh
     4561 snesnesnth qlpsvhgsvd ptrdietlne dqeseyvgds ecgtefseqy qcaetqrlnp
     4621 ldsggegeyk ykgsesylrh pnsylphyni htdtenetnp lngrlstles deeeddvvpy
     4681 gfpssrrnrc hkgdeadeig svittleern sllggatsns dlstnlceid dseyeiadqk
     4741 sngrlwlsgn gitqtsv
//
LOCUS       XP_034195558            4755 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195558
VERSION     XP_034195558.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339667.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195558.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4755
                     /product="FAT atypical cadherin kugelei isoform X6"
                     /calculated_mol_wt=527602
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1370..1456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1472..1561
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1477..1478,1527,1529,1557,1559..1560)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1569..1667
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1576..1577,1630,1632,1663,1665..1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1675..1767
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1780..1870
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1787..1788,1835,1837,1866,1868..1869)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1878..1983
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1885..1886,1945,1947,1980,1982..1983)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <2014..2079
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2092..2189
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2099..2100,2149,2151,2185,2187..2188)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2197..2288
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2299..2399
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2305..2306,2364,2366,2396,2398..2399)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2408..2507
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2415..2416,2470,2472,2503,2505..2506)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2515..2608
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2522..2523,2574,2576,2604,2606..2607)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2618..2711
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2623..2624,2676,2678,2707,2709..2710)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2719..2818
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2726..2727,2782,2784,2815,2817..2818)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2830..2919
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2834..2835,2882,2884,2915,2917..2918)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2927..3026
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2934..2935,2989,2991,3022,3024..3025)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3035..3127
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3041..3042,3093,3095,3123,3125..3126)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3136..3231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3142..3143,3194,3196,3227,3229..3230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3239..3335
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3246..3247,3298,3300,3331,3333..3334)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3343..3440
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3350..3351,3403,3405,3436,3438..3439)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3448..3545
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3455..3456,3508,3510,3541,3543..3544)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3554..3645
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3669..3743
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3926..3962
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3980..4136
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4176..4205
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4211..4250
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4211,4214,4230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4255..4284
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4288..4323
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4288,4291,4305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4755
                     /gene="kug"
                     /coded_by="XM_034339667.2:503..14770"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqksrnrs refdlsidtl lengtwetfs pdslsgdrif
     1381 rvlandkdig dngkiqysik ggrgkgkfki hpttgmvysq rgfeagqeye mmiraadsge
     1441 pqrshqtrvs vqvvevpkes enppvfktnn qrvevtesde vgflvalmqa tdkdgdslwy
     1501 dildgdkrde ffigrdngnv llakkldwet qnfynltigv tdsvhtavtq llvtvidind
     1561 hrpeftetty rvdisenvek gemilqlhat dededkkvfy slhaaqnqas leifhvdsvt
     1621 gsvtlnevld retieehvlt vmvkdqgtpa krnyarvivt vhdhndhape fiseiiqgkv
     1681 fetspigaav vqvwaidrdr gdnakityti tsgnvgnmfs idpelgvirv areldlsvss
     1741 eyillvkatd hgspalantv pvhvmvtmad napprfiqke lsaeiyenqq lgtyvkhvea
     1801 rstsslqfei vdgnkddtff vnpstgiivi kneldyektk fynltvaatn magakahcnv
     1861 ivhvldrndn aprflqatyt geisegasig slvltntstp lvikaedads elnallnydi
     1921 vedlprkyfh idsstgairt vmvldhesip kftfhvkvsd lgkpklsset takvmivvtd
     1981 vndcppkfle ndynttlllp tyknvavvrv navdpdsseg valrydiidg nkghtfdida
     2041 qtgvitvynp erikknyllr vrvsdgkyss vtqvnvavek sensglvfqk evyegsipen
     2101 strittvavv nvlgsalneh ivfsilnptd mfvigptsga irttgirfdr evcdhyeliv
     2161 eaksqmpdre kprvahvivn vtildindnc pmfvnlpyya vvsvdaqkgd vitkvhaidm
     2221 dsgdngevry elkkghgelf kvcrktgeis lkqnleghnr eyqltiaayd ggntpcsiev
     2281 pvnvkvidrs mpvfdkqfyt dsvlesieih splalsiqae splnrkliys itkgndfeef
     2341 aldfntapds nngpcviyvv deldyeqkkq yeltvratds vsgvyaevlv silvldvndc
     2401 ppeftqdsyn isvseaapfg tsvlkvssrd ndtginqqvr yaiqndtens selfhidpee
     2461 gviflkrsld hesheshhft viaidrgvps lsstahvwvt vidmndnppk feqpsytcsl
     2521 sehaergqfv tvvsasdpdy vdekltytiv ggneqqtyni dpstgiisli nmqnfgekkl
     2581 tmlnvsvtdn vytsfarvki eilpanrhnp kfpnpvvevt vlenqlpgrl vtsviasded
     2641 fgeygtviyt iasdlmkeif dinrltgeiv trkkldreeq klyeipvmat dgggrsgfvm
     2701 vrvkvgdend nspvfllrey kasihgnltv ntpflkvkaq dadeddaari vysiydlqss
     2761 eakalfginp dtgalflqks aipwenqlfe lfiraedkga mthhadvpls vyimgpqdip
     2821 plferkedkf fiseasaigt pitrlrtvtn ttvtyrivsg dedsphfmvd shgqvtlikp
     2881 ldretkdnhl igvlaetdss plltalaeis lqvldendha pefesnpygi ilaenieegt
     2941 silkiiahdd dlgsngevry sfgsdigela nvftidaytg wistlvqldk ekqpeykfqv
     3001 vatdngnpkh fartsvhvkl kdyndnppaf vddryeatvn edalpgtvvv klitvdkdsd
     3061 vntpiefyit sgdsrsqfqi rstgevyvak aldretidry eleivgtdgk yvfktrvtvq
     3121 vldvndnppy clryryreil segshpgtyv ltvlatdydd epnaklrfyl tgdnndkfsl
     3181 dketgvlktv gqldretqak ysltahvqdr dkpswecssq leilisdlnd nppkftmqty
     3241 satlpedvev gtlvtkvhat dddiginrki ryefidsadg qfliaadsgi vtlgksldre
     3301 tkamynvtiq aldqgtpqlt svtslivnvq dindnppefa skvyfskvpe iyavgtevar
     3361 vlatskdtgv nadvyysivg gnehkkfqid arsgvitiae qldyerardy fltiqavdgg
     3421 ipplsnhatv nitvidsndn apifsevsyr asvredakig ekvtqvfand ldseengnvs
     3481 yyiergdrqk qfsidqktgq itvaapldre eianyvlevh grdsgipmls sfvmvnievl
     3541 dandnpplfs lsnytavvqe dkplghtvlq fvvtdadiep nadpytfdfr sgnegdafrl
     3601 eqdgtlrtat kfnnrvkdky vlhirvfdng spplysdawv tikvieesqy ppvitplevi
     3661 insymdeypg giigkvhatd qdqydtlmys lvssspipnd lfkidkidgt lvalprldvg
     3721 eyrvnvtvtd gkfhsyslvk vnvdlitekm lensvivrfr evspedfmls hrkgfvrtlr
     3781 namncrpkdi viisvqpssd eanliksrai rqavhndldl lfavkksgta lasptfyvse
     3841 sirealnqri eeleestklv veeivrfkcn kgycvygdcq dkivldltqi tpvatdvmsf
     3901 vsprhkhkme cnckegyagn hcemvvneca rdpcplfkvc vpdssvqgys cqcpegfagq
     3961 tcdidiskch descyiprnp isfsgksyar yridkalirq tiedrlslsl rirtvqltgn
     4021 lmyaagkvdy nilevvngvv qykfdlgsge glvrvssvyv sdgqwheiql eresnsarlt
     4081 vdgkhvahgs apgindilnl qsddlylgae vrqhpsilgf edvqrgfigc mddvriarvs
     4141 vplhmsgass vavlkrfanv efscdattvl vppgicgsqp cqnggtckds ggdtyecqch
     4201 srfaglacei dtdpcasspc lyggrckivp eggdyscecv gpslsgkrce fgrycspnpc
     4261 ihggvceegn ngpickcqgf mgdrcetdvn ecdtnpctng gtcvneigty rcvcppnmtg
     4321 lncgnpayst piisshfnit weqlmwigva vilnvfliii flafrrirmk rtrarannin
     4381 netrkqivln sarphehefk rssklsnlev iqreppqcpp rpasytpssn nepapysnsa
     4441 avalnnldtl rsygsagdel enfppdylrn lnrttpvppp sltlanpvgg natgsdtdsl
     4501 hkpswqeqmq lasfitdttk ikngyhwdcs dwvrpsqnpl pnitevpgse vpdsssfhsn
     4561 esnesnthql psvhgsvdpt rdietlnedq eseyvgdsec gtefseqyqc aetqrlnpld
     4621 sggegeykyk gsesylrhpn sylphyniht dtenetnpln grlstlesde eeddvvpygf
     4681 pssrrnrchk gdeadeigsv ittleernsl lggatsnsdl stnlceidds eyeiadqksn
     4741 grlwlsgngi tqtsv
//
LOCUS       XP_034195559            4748 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034195559
VERSION     XP_034195559.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339668.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195559.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4748
                     /product="FAT atypical cadherin kugelei isoform X7"
                     /calculated_mol_wt=526753
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1370..1456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1472..1561
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1477..1478,1527,1529,1557,1559..1560)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1569..1667
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1576..1577,1630,1632,1663,1665..1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1675..1767
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1780..1870
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1787..1788,1835,1837,1866,1868..1869)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1878..1983
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1885..1886,1945,1947,1980,1982..1983)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <2014..2079
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2092..2189
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2099..2100,2149,2151,2185,2187..2188)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2197..2288
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2299..2399
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2305..2306,2364,2366,2396,2398..2399)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2408..2507
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2415..2416,2470,2472,2503,2505..2506)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2515..2608
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2522..2523,2574,2576,2604,2606..2607)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2618..2711
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2623..2624,2676,2678,2707,2709..2710)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2719..2818
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2726..2727,2782,2784,2815,2817..2818)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2830..2919
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2834..2835,2882,2884,2915,2917..2918)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2927..3026
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2934..2935,2989,2991,3022,3024..3025)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3035..3127
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3041..3042,3093,3095,3123,3125..3126)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3136..3231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3142..3143,3194,3196,3227,3229..3230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3239..3335
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3246..3247,3298,3300,3331,3333..3334)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3343..3440
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3350..3351,3403,3405,3436,3438..3439)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3448..3545
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3455..3456,3508,3510,3541,3543..3544)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3554..3645
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3669..3743
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3926..3962
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3980..4136
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4176..4205
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4211..4250
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4211,4214,4230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4255..4284
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4288..4323
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4288,4291,4305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4748
                     /gene="kug"
                     /coded_by="XM_034339668.2:503..14749"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqksrnrs refdlsidtl lengtwetfs pdslsgdrif
     1381 rvlandkdig dngkiqysik ggrgkgkfki hpttgmvysq rgfeagqeye mmiraadsge
     1441 pqrshqtrvs vqvvevpkes enppvfktnn qrvevtesde vgflvalmqa tdkdgdslwy
     1501 dildgdkrde ffigrdngnv llakkldwet qnfynltigv tdsvhtavtq llvtvidind
     1561 hrpeftetty rvdisenvek gemilqlhat dededkkvfy slhaaqnqas leifhvdsvt
     1621 gsvtlnevld retieehvlt vmvkdqgtpa krnyarvivt vhdhndhape fiseiiqgkv
     1681 fetspigaav vqvwaidrdr gdnakityti tsgnvgnmfs idpelgvirv areldlsvss
     1741 eyillvkatd hgspalantv pvhvmvtmad napprfiqke lsaeiyenqq lgtyvkhvea
     1801 rstsslqfei vdgnkddtff vnpstgiivi kneldyektk fynltvaatn magakahcnv
     1861 ivhvldrndn aprflqatyt geisegasig slvltntstp lvikaedads elnallnydi
     1921 vedlprkyfh idsstgairt vmvldhesip kftfhvkvsd lgkpklsset takvmivvtd
     1981 vndcppkfle ndynttlllp tyknvavvrv navdpdsseg valrydiidg nkghtfdida
     2041 qtgvitvynp erikknyllr vrvsdgkyss vtqvnvavek sensglvfqk evyegsipen
     2101 strittvavv nvlgsalneh ivfsilnptd mfvigptsga irttgirfdr evcdhyeliv
     2161 eaksqmpdre kprvahvivn vtildindnc pmfvnlpyya vvsvdaqkgd vitkvhaidm
     2221 dsgdngevry elkkghgelf kvcrktgeis lkqnleghnr eyqltiaayd ggntpcsiev
     2281 pvnvkvidrs mpvfdkqfyt dsvlesieih splalsiqae splnrkliys itkgndfeef
     2341 aldfntapds nngpcviyvv deldyeqkkq yeltvratds vsgvyaevlv silvldvndc
     2401 ppeftqdsyn isvseaapfg tsvlkvssrd ndtginqqvr yaiqndtens selfhidpee
     2461 gviflkrsld hesheshhft viaidrgvps lsstahvwvt vidmndnppk feqpsytcsl
     2521 sehaergqfv tvvsasdpdy vdekltytiv ggneqqtyni dpstgiisli nmqnfgekkl
     2581 tmlnvsvtdn vytsfarvki eilpanrhnp kfpnpvvevt vlenqlpgrl vtsviasded
     2641 fgeygtviyt iasdlmkeif dinrltgeiv trkkldreeq klyeipvmat dgggrsgfvm
     2701 vrvkvgdend nspvfllrey kasihgnltv ntpflkvkaq dadeddaari vysiydlqss
     2761 eakalfginp dtgalflqks aipwenqlfe lfiraedkga mthhadvpls vyimgpqdip
     2821 plferkedkf fiseasaigt pitrlrtvtn ttvtyrivsg dedsphfmvd shgqvtlikp
     2881 ldretkdnhl igvlaetdss plltalaeis lqvldendha pefesnpygi ilaenieegt
     2941 silkiiahdd dlgsngevry sfgsdigela nvftidaytg wistlvqldk ekqpeykfqv
     3001 vatdngnpkh fartsvhvkl kdyndnppaf vddryeatvn edalpgtvvv klitvdkdsd
     3061 vntpiefyit sgdsrsqfqi rstgevyvak aldretidry eleivgtdgk yvfktrvtvq
     3121 vldvndnppy clryryreil segshpgtyv ltvlatdydd epnaklrfyl tgdnndkfsl
     3181 dketgvlktv gqldretqak ysltahvqdr dkpswecssq leilisdlnd nppkftmqty
     3241 satlpedvev gtlvtkvhat dddiginrki ryefidsadg qfliaadsgi vtlgksldre
     3301 tkamynvtiq aldqgtpqlt svtslivnvq dindnppefa skvyfskvpe iyavgtevar
     3361 vlatskdtgv nadvyysivg gnehkkfqid arsgvitiae qldyerardy fltiqavdgg
     3421 ipplsnhatv nitvidsndn apifsevsyr asvredakig ekvtqvfand ldseengnvs
     3481 yyiergdrqk qfsidqktgq itvaapldre eianyvlevh grdsgipmls sfvmvnievl
     3541 dandnpplfs lsnytavvqe dkplghtvlq fvvtdadiep nadpytfdfr sgnegdafrl
     3601 eqdgtlrtat kfnnrvkdky vlhirvfdng spplysdawv tikvieesqy ppvitplevi
     3661 insymdeypg giigkvhatd qdqydtlmys lvssspipnd lfkidkidgt lvalprldvg
     3721 eyrvnvtvtd gkfhsyslvk vnvdlitekm lensvivrfr evspedfmls hrkgfvrtlr
     3781 namncrpkdi viisvqpssd eanliksrai rqavhndldl lfavkksgta lasptfyvse
     3841 sirealnqri eeleestklv veeivrfkcn kgycvygdcq dkivldltqi tpvatdvmsf
     3901 vsprhkhkme cnckegyagn hcemvvneca rdpcplfkvc vpdssvqgys cqcpegfagq
     3961 tcdidiskch descyiprnp isfsgksyar yridkalirq tiedrlslsl rirtvqltgn
     4021 lmyaagkvdy nilevvngvv qykfdlgsge glvrvssvyv sdgqwheiql eresnsarlt
     4081 vdgkhvahgs apgindilnl qsddlylgae vrqhpsilgf edvqrgfigc mddvriarvs
     4141 vplhmsgass vavlkrfanv efscdattvl vppgicgsqp cqnggtckds ggdtyecqch
     4201 srfaglacei dtdpcasspc lyggrckivp eggdyscecv gpslsgkrce fgrycspnpc
     4261 ihggvceegn ngpickcqgf mgdrcetdvn ecdtnpctng gtcvneigty rcvcppnmtg
     4321 lncgnpayst piisshfnit weqlmwigva vilnvfliii flafrrirmk rtrarannin
     4381 netrkqivln sarphehefk rssklsnlev iqreppqcpp rpasytpssn nepapysnsa
     4441 avalnnldtl rsygsagdel enfppdylrn lnrttpvppp sltlanpvgg natgsdtdsl
     4501 hkpswqeqmq lasfitdttk ikndwvrpsq nplpnitevp gsevpdsssf hsnesnesnt
     4561 hqlpsvhgsv dptrdietln edqeseyvgd secgtefseq yqcaetqrln pldsggegey
     4621 kykgsesylr hpnsylphyn ihtdtenetn plngrlstle sdeeeddvvp ygfpssrrnr
     4681 chkgdeadei gsvittleer nsllggatsn sdlstnlcei ddseyeiadq ksngrlwlsg
     4741 ngitqtsv
//
LOCUS       XP_034195560            4706 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034195560
VERSION     XP_034195560.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339669.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195560.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4706
                     /product="FAT atypical cadherin kugelei isoform X8"
                     /calculated_mol_wt=522153
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1370..1456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1472..1561
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1477..1478,1527,1529,1557,1559..1560)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1569..1667
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1576..1577,1630,1632,1663,1665..1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1675..1767
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1780..1870
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1787..1788,1835,1837,1866,1868..1869)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1878..1983
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1885..1886,1945,1947,1980,1982..1983)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <2014..2079
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2092..2189
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2099..2100,2149,2151,2185,2187..2188)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2197..2288
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2299..2399
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2305..2306,2364,2366,2396,2398..2399)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2408..2507
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2415..2416,2470,2472,2503,2505..2506)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2515..2608
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2522..2523,2574,2576,2604,2606..2607)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2618..2711
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2623..2624,2676,2678,2707,2709..2710)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2719..2818
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2726..2727,2782,2784,2815,2817..2818)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2830..2919
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2834..2835,2882,2884,2915,2917..2918)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2927..3026
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2934..2935,2989,2991,3022,3024..3025)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3035..3127
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3041..3042,3093,3095,3123,3125..3126)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3136..3231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3142..3143,3194,3196,3227,3229..3230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3239..3335
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3246..3247,3298,3300,3331,3333..3334)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3343..3440
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3350..3351,3403,3405,3436,3438..3439)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3448..3545
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3455..3456,3508,3510,3541,3543..3544)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3554..3645
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3669..3743
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3926..3962
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3980..4136
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4176..4205
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4211..4250
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4211,4214,4230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4255..4284
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4288..4323
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4288,4291,4305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4706
                     /gene="kug"
                     /coded_by="XM_034339669.2:503..14623"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqksrnrs refdlsidtl lengtwetfs pdslsgdrif
     1381 rvlandkdig dngkiqysik ggrgkgkfki hpttgmvysq rgfeagqeye mmiraadsge
     1441 pqrshqtrvs vqvvevpkes enppvfktnn qrvevtesde vgflvalmqa tdkdgdslwy
     1501 dildgdkrde ffigrdngnv llakkldwet qnfynltigv tdsvhtavtq llvtvidind
     1561 hrpeftetty rvdisenvek gemilqlhat dededkkvfy slhaaqnqas leifhvdsvt
     1621 gsvtlnevld retieehvlt vmvkdqgtpa krnyarvivt vhdhndhape fiseiiqgkv
     1681 fetspigaav vqvwaidrdr gdnakityti tsgnvgnmfs idpelgvirv areldlsvss
     1741 eyillvkatd hgspalantv pvhvmvtmad napprfiqke lsaeiyenqq lgtyvkhvea
     1801 rstsslqfei vdgnkddtff vnpstgiivi kneldyektk fynltvaatn magakahcnv
     1861 ivhvldrndn aprflqatyt geisegasig slvltntstp lvikaedads elnallnydi
     1921 vedlprkyfh idsstgairt vmvldhesip kftfhvkvsd lgkpklsset takvmivvtd
     1981 vndcppkfle ndynttlllp tyknvavvrv navdpdsseg valrydiidg nkghtfdida
     2041 qtgvitvynp erikknyllr vrvsdgkyss vtqvnvavek sensglvfqk evyegsipen
     2101 strittvavv nvlgsalneh ivfsilnptd mfvigptsga irttgirfdr evcdhyeliv
     2161 eaksqmpdre kprvahvivn vtildindnc pmfvnlpyya vvsvdaqkgd vitkvhaidm
     2221 dsgdngevry elkkghgelf kvcrktgeis lkqnleghnr eyqltiaayd ggntpcsiev
     2281 pvnvkvidrs mpvfdkqfyt dsvlesieih splalsiqae splnrkliys itkgndfeef
     2341 aldfntapds nngpcviyvv deldyeqkkq yeltvratds vsgvyaevlv silvldvndc
     2401 ppeftqdsyn isvseaapfg tsvlkvssrd ndtginqqvr yaiqndtens selfhidpee
     2461 gviflkrsld hesheshhft viaidrgvps lsstahvwvt vidmndnppk feqpsytcsl
     2521 sehaergqfv tvvsasdpdy vdekltytiv ggneqqtyni dpstgiisli nmqnfgekkl
     2581 tmlnvsvtdn vytsfarvki eilpanrhnp kfpnpvvevt vlenqlpgrl vtsviasded
     2641 fgeygtviyt iasdlmkeif dinrltgeiv trkkldreeq klyeipvmat dgggrsgfvm
     2701 vrvkvgdend nspvfllrey kasihgnltv ntpflkvkaq dadeddaari vysiydlqss
     2761 eakalfginp dtgalflqks aipwenqlfe lfiraedkga mthhadvpls vyimgpqdip
     2821 plferkedkf fiseasaigt pitrlrtvtn ttvtyrivsg dedsphfmvd shgqvtlikp
     2881 ldretkdnhl igvlaetdss plltalaeis lqvldendha pefesnpygi ilaenieegt
     2941 silkiiahdd dlgsngevry sfgsdigela nvftidaytg wistlvqldk ekqpeykfqv
     3001 vatdngnpkh fartsvhvkl kdyndnppaf vddryeatvn edalpgtvvv klitvdkdsd
     3061 vntpiefyit sgdsrsqfqi rstgevyvak aldretidry eleivgtdgk yvfktrvtvq
     3121 vldvndnppy clryryreil segshpgtyv ltvlatdydd epnaklrfyl tgdnndkfsl
     3181 dketgvlktv gqldretqak ysltahvqdr dkpswecssq leilisdlnd nppkftmqty
     3241 satlpedvev gtlvtkvhat dddiginrki ryefidsadg qfliaadsgi vtlgksldre
     3301 tkamynvtiq aldqgtpqlt svtslivnvq dindnppefa skvyfskvpe iyavgtevar
     3361 vlatskdtgv nadvyysivg gnehkkfqid arsgvitiae qldyerardy fltiqavdgg
     3421 ipplsnhatv nitvidsndn apifsevsyr asvredakig ekvtqvfand ldseengnvs
     3481 yyiergdrqk qfsidqktgq itvaapldre eianyvlevh grdsgipmls sfvmvnievl
     3541 dandnpplfs lsnytavvqe dkplghtvlq fvvtdadiep nadpytfdfr sgnegdafrl
     3601 eqdgtlrtat kfnnrvkdky vlhirvfdng spplysdawv tikvieesqy ppvitplevi
     3661 insymdeypg giigkvhatd qdqydtlmys lvssspipnd lfkidkidgt lvalprldvg
     3721 eyrvnvtvtd gkfhsyslvk vnvdlitekm lensvivrfr evspedfmls hrkgfvrtlr
     3781 namncrpkdi viisvqpssd eanliksrai rqavhndldl lfavkksgta lasptfyvse
     3841 sirealnqri eeleestklv veeivrfkcn kgycvygdcq dkivldltqi tpvatdvmsf
     3901 vsprhkhkme cnckegyagn hcemvvneca rdpcplfkvc vpdssvqgys cqcpegfagq
     3961 tcdidiskch descyiprnp isfsgksyar yridkalirq tiedrlslsl rirtvqltgn
     4021 lmyaagkvdy nilevvngvv qykfdlgsge glvrvssvyv sdgqwheiql eresnsarlt
     4081 vdgkhvahgs apgindilnl qsddlylgae vrqhpsilgf edvqrgfigc mddvriarvs
     4141 vplhmsgass vavlkrfanv efscdattvl vppgicgsqp cqnggtckds ggdtyecqch
     4201 srfaglacei dtdpcasspc lyggrckivp eggdyscecv gpslsgkrce fgrycspnpc
     4261 ihggvceegn ngpickcqgf mgdrcetdvn ecdtnpctng gtcvneigty rcvcppnmtg
     4321 lncgnpayst piisshfnit weqlmwigva vilnvfliii flafrrirmk rtrarannin
     4381 netrkqivln sarphehefk rssklsnlev iqreppqcpp rpasytpssn nepapysnsa
     4441 avalnnldtl rsygsagdel enfppdylrn lnrttpvppp sltlanpvgg natgsdtdsl
     4501 hkpswqeqmq lasfitdttk iknvhgsvdp trdietlned qeseyvgdse cgtefseqyq
     4561 caetqrlnpl dsggegeyky kgsesylrhp nsylphynih tdtenetnpl ngrlstlesd
     4621 eeeddvvpyg fpssrrnrch kgdeadeigs vittleerns llggatsnsd lstnlceidd
     4681 seyeiadqks ngrlwlsgng itqtsv
//
LOCUS       XP_034195561            4413 aa            linear   INV 03-SEP-2025
DEFINITION  FAT atypical cadherin kugelei isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_034195561
VERSION     XP_034195561.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339670.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195561.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4413
                     /product="FAT atypical cadherin kugelei isoform X9"
                     /calculated_mol_wt=489942
     Region          82..196
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(88..89,154,156,193,195..196)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          205..306
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(212..213,263,265,302,304..305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          330..417
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          425..524
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(432..433,485,487,520,522..523)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          532..630
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(539..540,591,593,626,628..629)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          640..>712
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          798..893
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(803..804,856,858,889,891..892)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          903..1001
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(908..909,959,961,997,999..1000)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1009..1107
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1016..1017,1070,1072,1103,1105..1106)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1119..1214
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1124..1125,1177,1179,1210,1212..1213)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1222..1319
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1229..1230,1282,1284,1315,1317..1318)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1370..1456
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1472..1561
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1477..1478,1527,1529,1557,1559..1560)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1569..1667
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1576..1577,1630,1632,1663,1665..1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1675..1767
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1780..1870
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1787..1788,1835,1837,1866,1868..1869)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1878..1983
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1885..1886,1945,1947,1980,1982..1983)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          <2014..2079
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2092..2189
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2099..2100,2149,2151,2185,2187..2188)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2197..2288
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          2299..2399
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2305..2306,2364,2366,2396,2398..2399)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2408..2507
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2415..2416,2470,2472,2503,2505..2506)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2515..2608
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2522..2523,2574,2576,2604,2606..2607)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2618..2711
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2623..2624,2676,2678,2707,2709..2710)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2719..2818
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2726..2727,2782,2784,2815,2817..2818)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2830..2919
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2834..2835,2882,2884,2915,2917..2918)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          2927..3026
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(2934..2935,2989,2991,3022,3024..3025)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3035..3127
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3041..3042,3093,3095,3123,3125..3126)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3136..3231
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3142..3143,3194,3196,3227,3229..3230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3239..3335
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3246..3247,3298,3300,3331,3333..3334)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3343..3440
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3350..3351,3403,3405,3436,3438..3439)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3448..3545
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(3455..3456,3508,3510,3541,3543..3544)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          3554..3645
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3669..3743
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          3926..3962
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          3980..4136
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          4176..4205
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4211..4250
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4211,4214,4230)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          4255..4284
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          4288..4323
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(4288,4291,4305)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..4413
                     /gene="kug"
                     /coded_by="XM_034339670.2:503..13744"
                     /db_xref="GeneID:117611644"
ORIGIN      
        1 mrrmkqrall awpwlwlwlf aatagspssd tfvsttfsss aisssvtrpf lssaiptsts
       61 vsftedfalp ldqefrftqp lynvsipens igktyvvpee rmgiklaspd tnfdvrfrim
      121 sgdrdkffka eertvgdfcf llirtrtgnv dvlnrerkdr yvlevratas rgegknrvti
      181 leadttivvt vldtndlnpl fypteykqni tedtplhrsi lrvmaedadl grngeiyysf
      241 aeetdqfavh pvsgvitltr plryseraih elvvlakdrg atfrsgggsr astakvtikv
      301 rqvnlhapei dvhhlpdive hsnadiyaiv rvtdsdegvh gqiasldivg gdpaghfrvr
      361 paggphsgey nievlhlldr etavqgynlt lramdrgvpq rssyksvpvh ladlndnapv
      421 fsreiyevkv petapintpv irlkvtdade gknalvflei vggneggefy vnaetgmlyt
      481 amnldaekka fytltvsavd qgnagtrkqs sakvkinvvd tndndptfeq semevwiden
      541 epagtsvvki takdrdsgen ayisysidni qkvpfeidhf sgivrtkqvl dyetmkreyl
      601 lrvrvgdwgl pyrrqaemrl hvkvrdindn rpqferiect ghvpryvpig seiitvsaid
      661 fdagniisyr ivsgnedgcf aldsasgvls vacdlsdvkv tdrvvnvtat dgshfadvnp
      721 vhmhlvnakr nlgsqgrilt dqsgafecrd tgvarrltea iaaaernnvp skddeyaltp
      781 srygenvhap efidfpneik vnesvkigtt lvkirardkd ldyngklvfc issgdrdsvf
      841 gidpdtgkln vigyldrere neyflnisvy dlgkpqksas rmlpvtildv ndnapkfeks
      901 lasfriseta lngtnvwran atdadlgena rvtyslvtet ndfrvdpvtg vlsvfgrldr
      961 erqeiyelri raqdnggkgt dtpplysdal vrvtvddvnd naptfalssy tvkiredvpi
     1021 wtvvavvdat dpdegaggdv eyflsdames egffkvdkvs gtirtvqsld feerqvhtlt
     1081 ivakdrgeps lssetmviie vvdvnenlha pvfddfvvsa svfenqpigt lvttvrakda
     1141 dplggdsrig ytirggdgig ifsidnegni ktkavldves krgywltvya qdhgvvplss
     1201 slqvyvevld endntpltev pvyypsvpen spagvsvlqi rafdrdvspq qftfsitsgn
     1261 pegyflinst tglittsgrk ldrenqaehv levtvrddgr pslssttrvv isvadindhg
     1321 pefeqkfytv qipaspstdk plfqksrnrs refdlsidtl lengtwetfs pdslsgdrif
     1381 rvlandkdig dngkiqysik ggrgkgkfki hpttgmvysq rgfeagqeye mmiraadsge
     1441 pqrshqtrvs vqvvevpkes enppvfktnn qrvevtesde vgflvalmqa tdkdgdslwy
     1501 dildgdkrde ffigrdngnv llakkldwet qnfynltigv tdsvhtavtq llvtvidind
     1561 hrpeftetty rvdisenvek gemilqlhat dededkkvfy slhaaqnqas leifhvdsvt
     1621 gsvtlnevld retieehvlt vmvkdqgtpa krnyarvivt vhdhndhape fiseiiqgkv
     1681 fetspigaav vqvwaidrdr gdnakityti tsgnvgnmfs idpelgvirv areldlsvss
     1741 eyillvkatd hgspalantv pvhvmvtmad napprfiqke lsaeiyenqq lgtyvkhvea
     1801 rstsslqfei vdgnkddtff vnpstgiivi kneldyektk fynltvaatn magakahcnv
     1861 ivhvldrndn aprflqatyt geisegasig slvltntstp lvikaedads elnallnydi
     1921 vedlprkyfh idsstgairt vmvldhesip kftfhvkvsd lgkpklsset takvmivvtd
     1981 vndcppkfle ndynttlllp tyknvavvrv navdpdsseg valrydiidg nkghtfdida
     2041 qtgvitvynp erikknyllr vrvsdgkyss vtqvnvavek sensglvfqk evyegsipen
     2101 strittvavv nvlgsalneh ivfsilnptd mfvigptsga irttgirfdr evcdhyeliv
     2161 eaksqmpdre kprvahvivn vtildindnc pmfvnlpyya vvsvdaqkgd vitkvhaidm
     2221 dsgdngevry elkkghgelf kvcrktgeis lkqnleghnr eyqltiaayd ggntpcsiev
     2281 pvnvkvidrs mpvfdkqfyt dsvlesieih splalsiqae splnrkliys itkgndfeef
     2341 aldfntapds nngpcviyvv deldyeqkkq yeltvratds vsgvyaevlv silvldvndc
     2401 ppeftqdsyn isvseaapfg tsvlkvssrd ndtginqqvr yaiqndtens selfhidpee
     2461 gviflkrsld hesheshhft viaidrgvps lsstahvwvt vidmndnppk feqpsytcsl
     2521 sehaergqfv tvvsasdpdy vdekltytiv ggneqqtyni dpstgiisli nmqnfgekkl
     2581 tmlnvsvtdn vytsfarvki eilpanrhnp kfpnpvvevt vlenqlpgrl vtsviasded
     2641 fgeygtviyt iasdlmkeif dinrltgeiv trkkldreeq klyeipvmat dgggrsgfvm
     2701 vrvkvgdend nspvfllrey kasihgnltv ntpflkvkaq dadeddaari vysiydlqss
     2761 eakalfginp dtgalflqks aipwenqlfe lfiraedkga mthhadvpls vyimgpqdip
     2821 plferkedkf fiseasaigt pitrlrtvtn ttvtyrivsg dedsphfmvd shgqvtlikp
     2881 ldretkdnhl igvlaetdss plltalaeis lqvldendha pefesnpygi ilaenieegt
     2941 silkiiahdd dlgsngevry sfgsdigela nvftidaytg wistlvqldk ekqpeykfqv
     3001 vatdngnpkh fartsvhvkl kdyndnppaf vddryeatvn edalpgtvvv klitvdkdsd
     3061 vntpiefyit sgdsrsqfqi rstgevyvak aldretidry eleivgtdgk yvfktrvtvq
     3121 vldvndnppy clryryreil segshpgtyv ltvlatdydd epnaklrfyl tgdnndkfsl
     3181 dketgvlktv gqldretqak ysltahvqdr dkpswecssq leilisdlnd nppkftmqty
     3241 satlpedvev gtlvtkvhat dddiginrki ryefidsadg qfliaadsgi vtlgksldre
     3301 tkamynvtiq aldqgtpqlt svtslivnvq dindnppefa skvyfskvpe iyavgtevar
     3361 vlatskdtgv nadvyysivg gnehkkfqid arsgvitiae qldyerardy fltiqavdgg
     3421 ipplsnhatv nitvidsndn apifsevsyr asvredakig ekvtqvfand ldseengnvs
     3481 yyiergdrqk qfsidqktgq itvaapldre eianyvlevh grdsgipmls sfvmvnievl
     3541 dandnpplfs lsnytavvqe dkplghtvlq fvvtdadiep nadpytfdfr sgnegdafrl
     3601 eqdgtlrtat kfnnrvkdky vlhirvfdng spplysdawv tikvieesqy ppvitplevi
     3661 insymdeypg giigkvhatd qdqydtlmys lvssspipnd lfkidkidgt lvalprldvg
     3721 eyrvnvtvtd gkfhsyslvk vnvdlitekm lensvivrfr evspedfmls hrkgfvrtlr
     3781 namncrpkdi viisvqpssd eanliksrai rqavhndldl lfavkksgta lasptfyvse
     3841 sirealnqri eeleestklv veeivrfkcn kgycvygdcq dkivldltqi tpvatdvmsf
     3901 vsprhkhkme cnckegyagn hcemvvneca rdpcplfkvc vpdssvqgys cqcpegfagq
     3961 tcdidiskch descyiprnp isfsgksyar yridkalirq tiedrlslsl rirtvqltgn
     4021 lmyaagkvdy nilevvngvv qykfdlgsge glvrvssvyv sdgqwheiql eresnsarlt
     4081 vdgkhvahgs apgindilnl qsddlylgae vrqhpsilgf edvqrgfigc mddvriarvs
     4141 vplhmsgass vavlkrfanv efscdattvl vppgicgsqp cqnggtckds ggdtyecqch
     4201 srfaglacei dtdpcasspc lyggrckivp eggdyscecv gpslsgkrce fgrycspnpc
     4261 ihggvceegn ngpickcqgf mgdrcetdvn ecdtnpctng gtcvneigty rcvcppnmtg
     4321 lncgnpayst piisshfnit weqlmwigva vilnvfliii flafrrirmk rtrarannin
     4381 netrkqivln sarphehefk rssklsnlev iqt
//
LOCUS       XP_034195563             693 aa            linear   INV 03-SEP-2025
DEFINITION  TNF receptor associated protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034195563
VERSION     XP_034195563.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339672.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195563.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..693
                     /product="TNF receptor associated protein 1"
                     /calculated_mol_wt=78844
     Region          75..691
                     /region_name="HtpG"
                     /note="Molecular chaperone, HSP90 family
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG0326"
                     /db_xref="CDD:440095"
     CDS             1..693
                     /gene="Trap1"
                     /coded_by="XM_034339672.2:33..2114"
                     /db_xref="GeneID:117611645"
ORIGIN      
        1 matafrlrla ikcgrnvsrp lsrhlerkil ekqcqqfval agtsvrylss qaatqttesh
       61 niikdaekvi gesdkhefqs etqmllqiva kslysekevf lrelisnasd aleklrymql
      121 tdgkaaeiig srnleihiat dkqnrtltiq dtgigmtree lisnlgtiar sgsraflekl
      181 kekqdagdas qiigqfgvgf ystfmvadkv evftksyapd veglcwtsdg sstfkiakae
      241 gvqpgtkivi hlkadcrefs dedtvnrlit kysnfigcpi ylngrrvnti qplwmldpkd
      301 itplqheefy rfigncfdkp rfvlhyttda plnirallyf peekpplydf ikndtsgvsl
      361 ysrkiliknr aenilpkwlr fikgvvdsed iplnlsrell qnstligklr nvltarvlky
      421 lhersqkqpe eyikfykdhs iflkegiist dnqkekeeia kllrfessat apgevisldd
      481 yckrispnqk kinylvapsr slaeqspyye slkkrnievl fcyeahddvi flhlrnyksy
      541 tltsieeemr eadssksdss giidqgeisn llnymkkvla gkahdvkitn rleshpcvit
      601 vqdlasarhf triqtrqlkd dalyhllkps feinpnhpiv kklcsltntn skladllikq
      661 lftnsmveag lidnprllva smnellteal eky
//
LOCUS       XP_034195528             208 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 43 [Osmia lignaria lignaria].
ACCESSION   XP_034195528
VERSION     XP_034195528.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339637.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="coiled-coil domain-containing protein 43"
                     /calculated_mol_wt=23475
     CDS             1..208
                     /gene="LOC117611623"
                     /coded_by="XM_034339637.2:178..804"
                     /db_xref="GeneID:117611623"
ORIGIN      
        1 mavvtnsfds wlskklqaln tdegvfgsyi kgilegdete dektealesi lsgitdesis
       61 khvaeilsaw tkwlpvedva askvpvedvd vrlarmlesq slptttqksy teeerkirea
      121 ilaqysqmsd eehsegeged egasggdcgi ekntnvaaii qqekerreka kqdsqkkkek
      181 dkedrekqkq lkeekkekrk tqkgerrr
//
LOCUS       XP_034195529             270 aa            linear   INV 03-SEP-2025
DEFINITION  trypsin 5G1 [Osmia lignaria lignaria].
ACCESSION   XP_034195529
VERSION     XP_034195529.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339638.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195529.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="trypsin 5G1"
                     /calculated_mol_wt=29469
     Region          40..266
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            40
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(87,132,225)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(219,240,242)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..270
                     /gene="LOC117611625"
                     /coded_by="XM_034339638.2:69..881"
                     /db_xref="GeneID:117611625"
ORIGIN      
        1 myssllmifg fsvlctasgy gnyqkrivpm qeqdspgsri iggaqagiee ypfavslqnn
       61 gtffgheveh fcggsiigek wiitsaqcal rirvkgfhvr agtshyyeng eifrvksvti
      121 hpafneniyd ydvglielad sikydnmkqs iklpqshsmi edgvqvqvlg wgasrllgpv
      181 seellrstmq kinnencqqa sggdiltnrm fcalsngagp cvgdsgspli fenmlfgits
      241 wsrscsskyp taftatsevk dwitevtgia
//
LOCUS       XP_076546619             610 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546619
VERSION     XP_076546619.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690504.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="CUGBP Elav-like family member 1-A isoform X1"
                     /calculated_mol_wt=65381
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          518..609
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..610
                     /gene="LOC117611675"
                     /coded_by="XM_076690504.1:449..2281"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn tageyantgv spmsmqnlvt laamtggngn
      421 lqvspntlsg lanstagmsl svllgktgnt gstggslspv tslalnsltg tplnglqdsl
      481 snaysslqqy agfpaftaaa aaaaaaaqka kftntdkqie gpegcnlfiy hlpqefndtd
      541 lvttflpfgn visakvfidk qtqlskcfgf vsydnaasaq aaiqamngfq igmkrlkvql
      601 krskdaskpy
//
LOCUS       XP_076546620             610 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546620
VERSION     XP_076546620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690505.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="CUGBP Elav-like family member 1-A isoform X1"
                     /calculated_mol_wt=65381
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          518..609
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..610
                     /gene="LOC117611675"
                     /coded_by="XM_076690505.1:449..2281"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn tageyantgv spmsmqnlvt laamtggngn
      421 lqvspntlsg lanstagmsl svllgktgnt gstggslspv tslalnsltg tplnglqdsl
      481 snaysslqqy agfpaftaaa aaaaaaaqka kftntdkqie gpegcnlfiy hlpqefndtd
      541 lvttflpfgn visakvfidk qtqlskcfgf vsydnaasaq aaiqamngfq igmkrlkvql
      601 krskdaskpy
//
LOCUS       XP_076546621             610 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546621
VERSION     XP_076546621.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690506.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="CUGBP Elav-like family member 1-A isoform X1"
                     /calculated_mol_wt=65381
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          518..609
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..610
                     /gene="LOC117611675"
                     /coded_by="XM_076690506.1:449..2281"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn tageyantgv spmsmqnlvt laamtggngn
      421 lqvspntlsg lanstagmsl svllgktgnt gstggslspv tslalnsltg tplnglqdsl
      481 snaysslqqy agfpaftaaa aaaaaaaqka kftntdkqie gpegcnlfiy hlpqefndtd
      541 lvttflpfgn visakvfidk qtqlskcfgf vsydnaasaq aaiqamngfq igmkrlkvql
      601 krskdaskpy
//
LOCUS       XP_076546622             610 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546622
VERSION     XP_076546622.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690507.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..610
                     /product="CUGBP Elav-like family member 1-A isoform X1"
                     /calculated_mol_wt=65381
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          518..609
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..610
                     /gene="LOC117611675"
                     /coded_by="XM_076690507.1:449..2281"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn tageyantgv spmsmqnlvt laamtggngn
      421 lqvspntlsg lanstagmsl svllgktgnt gstggslspv tslalnsltg tplnglqdsl
      481 snaysslqqy agfpaftaaa aaaaaaaqka kftntdkqie gpegcnlfiy hlpqefndtd
      541 lvttflpfgn visakvfidk qtqlskcfgf vsydnaasaq aaiqamngfq igmkrlkvql
      601 krskdaskpy
//
LOCUS       XP_076546623             607 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546623
VERSION     XP_076546623.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690508.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..607
                     /product="CUGBP Elav-like family member 1-A isoform X2"
                     /calculated_mol_wt=65032
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          515..606
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..607
                     /gene="LOC117611675"
                     /coded_by="XM_076690508.1:449..2272"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn taantgvspm smqnlvtlaa mtggngnlqv
      421 spntlsglan stagmslsvl lgktgntgst ggslspvtsl alnsltgtpl nglqdslsna
      481 ysslqqyagf paftaaaaaa aaaaqkakft ntdkqiegpe gcnlfiyhlp qefndtdlvt
      541 tflpfgnvis akvfidkqtq lskcfgfvsy dnaasaqaai qamngfqigm krlkvqlkrs
      601 kdaskpy
//
LOCUS       XP_076546624             600 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546624
VERSION     XP_076546624.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690509.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..600
                     /product="CUGBP Elav-like family member 1-A isoform X3"
                     /calculated_mol_wt=64539
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          414..>592
                     /region_name="sex-lethal"
                     /note="sex-lethal family splicing factor; TIGR01659"
                     /db_xref="CDD:273740"
     Region          508..599
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..600
                     /gene="LOC117611675"
                     /coded_by="XM_076690509.1:448..2250"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn tageyantgv spmsmqnlvt laamtggngn
      421 lqvspntlsg lanstagmsl wstggslspv tslalnsltg tplnglqdsl snaysslqqy
      481 agfpaftaaa aaaaaaaqka kftntdkqie gpegcnlfiy hlpqefndtd lvttflpfgn
      541 visakvfidk qtqlskcfgf vsydnaasaq aaiqamngfq igmkrlkvql krskdaskpy
//
LOCUS       XP_076546625             596 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546625
VERSION     XP_076546625.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690510.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="CUGBP Elav-like family member 1 isoform X4"
                     /calculated_mol_wt=64021
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          504..595
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..596
                     /gene="LOC117611675"
                     /coded_by="XM_076690510.1:448..2238"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn tageyantgv spmsmqnlvt laamtggngn
      421 lqvspntlsg lanstagstg gslspvtsla lnsltgtpln glqdslsnay sslqqyagfp
      481 aftaaaaaaa aaaqkakftn tdkqiegpeg cnlfiyhlpq efndtdlvtt flpfgnvisa
      541 kvfidkqtql skcfgfvsyd naasaqaaiq amngfqigmk rlkvqlkrsk daskpy
//
LOCUS       XP_076546626             588 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546626
VERSION     XP_076546626.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690511.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="CUGBP Elav-like family member 1-A isoform X5"
                     /calculated_mol_wt=62951
     Region          84..165
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          171..251
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          496..587
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..588
                     /gene="LOC117611675"
                     /coded_by="XM_076690511.1:1498..3264"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mkmlqslnal agkispgspt dsnankvhmh nnnnnnnnnv vhhhhpyskq qtqqpspssq
       61 ssnsdqkent imnnnsveqp dpdnikmfvg qvphdmdend lrklfeefgr vhqinilrdk
      121 itgshrgccf vtfytrkaal daqnalhnvk tfsgmrhpiq mkpadsenrn erklfvgmls
      181 kkftendvrn mfsvygtiee csvlrdstgk skacafvtfa skqyainaik alhhsqtmeg
      241 cssplvvkfa dtqkekdqkr mqqlqtnlwn iagvnmaphy ltndtptlap aslqllqqlq
      301 attsaatpva asaganalss vqhqlllqqh lglgaatpah aaapalpspp einpanlqgl
      361 atlaslsnta geyantgvsp msmqnlvtla amtggngnlq vspntlsgla nstagmslsv
      421 llgktgntgs tggslspvts lalnsltgtp lnglqdslsn aysslqqyag fpaftaaaaa
      481 aaaaaqkakf tntdkqiegp egcnlfiyhl pqefndtdlv ttflpfgnvi sakvfidkqt
      541 qlskcfgfvs ydnaasaqaa iqamngfqig mkrlkvqlkr skdaskpy
//
LOCUS       XP_076546627             588 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546627
VERSION     XP_076546627.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690512.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="CUGBP Elav-like family member 1-A isoform X5"
                     /calculated_mol_wt=62951
     Region          84..165
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          171..251
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          496..587
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..588
                     /gene="LOC117611675"
                     /coded_by="XM_076690512.1:1238..3004"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mkmlqslnal agkispgspt dsnankvhmh nnnnnnnnnv vhhhhpyskq qtqqpspssq
       61 ssnsdqkent imnnnsveqp dpdnikmfvg qvphdmdend lrklfeefgr vhqinilrdk
      121 itgshrgccf vtfytrkaal daqnalhnvk tfsgmrhpiq mkpadsenrn erklfvgmls
      181 kkftendvrn mfsvygtiee csvlrdstgk skacafvtfa skqyainaik alhhsqtmeg
      241 cssplvvkfa dtqkekdqkr mqqlqtnlwn iagvnmaphy ltndtptlap aslqllqqlq
      301 attsaatpva asaganalss vqhqlllqqh lglgaatpah aaapalpspp einpanlqgl
      361 atlaslsnta geyantgvsp msmqnlvtla amtggngnlq vspntlsgla nstagmslsv
      421 llgktgntgs tggslspvts lalnsltgtp lnglqdslsn aysslqqyag fpaftaaaaa
      481 aaaaaqkakf tntdkqiegp egcnlfiyhl pqefndtdlv ttflpfgnvi sakvfidkqt
      541 qlskcfgfvs ydnaasaqaa iqamngfqig mkrlkvqlkr skdaskpy
//
LOCUS       XP_076546628             588 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546628
VERSION     XP_076546628.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690513.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="CUGBP Elav-like family member 1-A isoform X5"
                     /calculated_mol_wt=62951
     Region          84..165
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          171..251
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          496..587
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..588
                     /gene="LOC117611675"
                     /coded_by="XM_076690513.1:864..2630"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mkmlqslnal agkispgspt dsnankvhmh nnnnnnnnnv vhhhhpyskq qtqqpspssq
       61 ssnsdqkent imnnnsveqp dpdnikmfvg qvphdmdend lrklfeefgr vhqinilrdk
      121 itgshrgccf vtfytrkaal daqnalhnvk tfsgmrhpiq mkpadsenrn erklfvgmls
      181 kkftendvrn mfsvygtiee csvlrdstgk skacafvtfa skqyainaik alhhsqtmeg
      241 cssplvvkfa dtqkekdqkr mqqlqtnlwn iagvnmaphy ltndtptlap aslqllqqlq
      301 attsaatpva asaganalss vqhqlllqqh lglgaatpah aaapalpspp einpanlqgl
      361 atlaslsnta geyantgvsp msmqnlvtla amtggngnlq vspntlsgla nstagmslsv
      421 llgktgntgs tggslspvts lalnsltgtp lnglqdslsn aysslqqyag fpaftaaaaa
      481 aaaaaqkakf tntdkqiegp egcnlfiyhl pqefndtdlv ttflpfgnvi sakvfidkqt
      541 qlskcfgfvs ydnaasaqaa iqamngfqig mkrlkvqlkr skdaskpy
//
LOCUS       XP_076546629             588 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546629
VERSION     XP_076546629.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690514.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="CUGBP Elav-like family member 1-A isoform X5"
                     /calculated_mol_wt=62951
     Region          84..165
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          171..251
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          496..587
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..588
                     /gene="LOC117611675"
                     /coded_by="XM_076690514.1:604..2370"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mkmlqslnal agkispgspt dsnankvhmh nnnnnnnnnv vhhhhpyskq qtqqpspssq
       61 ssnsdqkent imnnnsveqp dpdnikmfvg qvphdmdend lrklfeefgr vhqinilrdk
      121 itgshrgccf vtfytrkaal daqnalhnvk tfsgmrhpiq mkpadsenrn erklfvgmls
      181 kkftendvrn mfsvygtiee csvlrdstgk skacafvtfa skqyainaik alhhsqtmeg
      241 cssplvvkfa dtqkekdqkr mqqlqtnlwn iagvnmaphy ltndtptlap aslqllqqlq
      301 attsaatpva asaganalss vqhqlllqqh lglgaatpah aaapalpspp einpanlqgl
      361 atlaslsnta geyantgvsp msmqnlvtla amtggngnlq vspntlsgla nstagmslsv
      421 llgktgntgs tggslspvts lalnsltgtp lnglqdslsn aysslqqyag fpaftaaaaa
      481 aaaaaqkakf tntdkqiegp egcnlfiyhl pqefndtdlv ttflpfgnvi sakvfidkqt
      541 qlskcfgfvs ydnaasaqaa iqamngfqig mkrlkvqlkr skdaskpy
//
LOCUS       XP_076546630             588 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546630
VERSION     XP_076546630.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690515.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="CUGBP Elav-like family member 1-A isoform X5"
                     /calculated_mol_wt=62951
     Region          84..165
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          171..251
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          496..587
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..588
                     /gene="LOC117611675"
                     /coded_by="XM_076690515.1:1148..2914"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mkmlqslnal agkispgspt dsnankvhmh nnnnnnnnnv vhhhhpyskq qtqqpspssq
       61 ssnsdqkent imnnnsveqp dpdnikmfvg qvphdmdend lrklfeefgr vhqinilrdk
      121 itgshrgccf vtfytrkaal daqnalhnvk tfsgmrhpiq mkpadsenrn erklfvgmls
      181 kkftendvrn mfsvygtiee csvlrdstgk skacafvtfa skqyainaik alhhsqtmeg
      241 cssplvvkfa dtqkekdqkr mqqlqtnlwn iagvnmaphy ltndtptlap aslqllqqlq
      301 attsaatpva asaganalss vqhqlllqqh lglgaatpah aaapalpspp einpanlqgl
      361 atlaslsnta geyantgvsp msmqnlvtla amtggngnlq vspntlsgla nstagmslsv
      421 llgktgntgs tggslspvts lalnsltgtp lnglqdslsn aysslqqyag fpaftaaaaa
      481 aaaaaqkakf tntdkqiegp egcnlfiyhl pqefndtdlv ttflpfgnvi sakvfidkqt
      541 qlskcfgfvs ydnaasaqaa iqamngfqig mkrlkvqlkr skdaskpy
//
LOCUS       XP_076546631             588 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546631
VERSION     XP_076546631.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690516.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..588
                     /product="CUGBP Elav-like family member 1-A isoform X5"
                     /calculated_mol_wt=62951
     Region          84..165
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          171..251
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          496..587
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..588
                     /gene="LOC117611675"
                     /coded_by="XM_076690516.1:254..2020"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mkmlqslnal agkispgspt dsnankvhmh nnnnnnnnnv vhhhhpyskq qtqqpspssq
       61 ssnsdqkent imnnnsveqp dpdnikmfvg qvphdmdend lrklfeefgr vhqinilrdk
      121 itgshrgccf vtfytrkaal daqnalhnvk tfsgmrhpiq mkpadsenrn erklfvgmls
      181 kkftendvrn mfsvygtiee csvlrdstgk skacafvtfa skqyainaik alhhsqtmeg
      241 cssplvvkfa dtqkekdqkr mqqlqtnlwn iagvnmaphy ltndtptlap aslqllqqlq
      301 attsaatpva asaganalss vqhqlllqqh lglgaatpah aaapalpspp einpanlqgl
      361 atlaslsnta geyantgvsp msmqnlvtla amtggngnlq vspntlsgla nstagmslsv
      421 llgktgntgs tggslspvts lalnsltgtp lnglqdslsn aysslqqyag fpaftaaaaa
      481 aaaaaqkakf tntdkqiegp egcnlfiyhl pqefndtdlv ttflpfgnvi sakvfidkqt
      541 qlskcfgfvs ydnaasaqaa iqamngfqig mkrlkvqlkr skdaskpy
//
LOCUS       XP_076546632             586 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546632
VERSION     XP_076546632.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690517.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="CUGBP Elav-like family member 1 isoform X6"
                     /calculated_mol_wt=63205
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          494..585
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..586
                     /gene="LOC117611675"
                     /coded_by="XM_076690517.1:448..2208"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn tageyantgv spmsmqnlvt laamtggngn
      421 lqvspnrstg gslspvtsla lnsltgtpln glqdslsnay sslqqyagfp aftaaaaaaa
      481 aaaqkakftn tdkqiegpeg cnlfiyhlpq efndtdlvtt flpfgnvisa kvfidkqtql
      541 skcfgfvsyd naasaqaaiq amngfqigmk rlkvqlkrsk daskpy
//
LOCUS       XP_076546633             583 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 2 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546633
VERSION     XP_076546633.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690518.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..583
                     /product="CUGBP Elav-like family member 2 isoform X7"
                     /calculated_mol_wt=62855
     Region          106..187
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          193..273
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          491..582
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..583
                     /gene="LOC117611675"
                     /coded_by="XM_076690518.1:448..2199"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigineaeg rghrepvarr tpmkmlqsln alagkispgs ptdsnankvh mhnnnnnnnn
       61 nvvhhhhpys kqqtqqpsps sqssnsdqke ntimnnnsve qpdpdnikmf vgqvphdmde
      121 ndlrklfeef grvhqinilr dkitgshrgc cfvtfytrka aldaqnalhn vktfsgmrhp
      181 iqmkpadsen rnerklfvgm lskkftendv rnmfsvygti eecsvlrdst gkskacafvt
      241 faskqyaina ikalhhsqtm egcssplvvk fadtqkekdq krmqqlqtnl wniagvnmap
      301 hyltndtptl apaslqllqq lqattsaatp vaasaganal ssvqhqlllq qhlglgaatp
      361 ahaaapalps ppeinpanlq glatlaslsn taantgvspm smqnlvtlaa mtggngnlqv
      421 spnrstggsl spvtslalns ltgtplnglq dslsnayssl qqyagfpaft aaaaaaaaaa
      481 qkakftntdk qiegpegcnl fiyhlpqefn dtdlvttflp fgnvisakvf idkqtqlskc
      541 fgfvsydnaa saqaaiqamn gfqigmkrlk vqlkrskdas kpy
//
LOCUS       XP_076546634             522 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546634
VERSION     XP_076546634.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690519.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="CUGBP Elav-like family member 1-A isoform X8"
                     /calculated_mol_wt=55655
     Region          18..99
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          105..185
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          430..521
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..522
                     /gene="LOC117611675"
                     /coded_by="XM_076690519.1:449..2017"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mtigimnnns veqpdpdnik mfvgqvphdm dendlrklfe efgrvhqini lrdkitgshr
       61 gccfvtfytr kaaldaqnal hnvktfsgmr hpiqmkpads enrnerklfv gmlskkften
      121 dvrnmfsvyg tieecsvlrd stgkskacaf vtfaskqyai naikalhhsq tmegcssplv
      181 vkfadtqkek dqkrmqqlqt nlwniagvnm aphyltndtp tlapaslqll qqlqattsaa
      241 tpvaasagan alssvqhqll lqqhlglgaa tpahaaapal psppeinpan lqglatlasl
      301 sntageyant gvspmsmqnl vtlaamtggn gnlqvspntl sglanstagm slsvllgktg
      361 ntgstggsls pvtslalnsl tgtplnglqd slsnaysslq qyagfpafta aaaaaaaaaq
      421 kakftntdkq iegpegcnlf iyhlpqefnd tdlvttflpf gnvisakvfi dkqtqlskcf
      481 gfvsydnaas aqaaiqamng fqigmkrlkv qlkrskdask py
//
LOCUS       XP_076546635             517 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546635
VERSION     XP_076546635.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690520.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..517
                     /product="CUGBP Elav-like family member 1-A isoform X9"
                     /calculated_mol_wt=55140
     Region          13..94
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          100..180
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          425..516
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..517
                     /gene="LOC117611675"
                     /coded_by="XM_076690520.1:1098..2651"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mnnnsveqpd pdnikmfvgq vphdmdendl rklfeefgrv hqinilrdki tgshrgccfv
       61 tfytrkaald aqnalhnvkt fsgmrhpiqm kpadsenrne rklfvgmlsk kftendvrnm
      121 fsvygtieec svlrdstgks kacafvtfas kqyainaika lhhsqtmegc ssplvvkfad
      181 tqkekdqkrm qqlqtnlwni agvnmaphyl tndtptlapa slqllqqlqa ttsaatpvaa
      241 saganalssv qhqlllqqhl glgaatpaha aapalpsppe inpanlqgla tlaslsntag
      301 eyantgvspm smqnlvtlaa mtggngnlqv spntlsglan stagmslsvl lgktgntgst
      361 ggslspvtsl alnsltgtpl nglqdslsna ysslqqyagf paftaaaaaa aaaaqkakft
      421 ntdkqiegpe gcnlfiyhlp qefndtdlvt tflpfgnvis akvfidkqtq lskcfgfvsy
      481 dnaasaqaai qamngfqigm krlkvqlkrs kdaskpy
//
LOCUS       XP_076546636             517 aa            linear   INV 03-SEP-2025
DEFINITION  CUGBP Elav-like family member 1-A isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546636
VERSION     XP_076546636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690521.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..517
                     /product="CUGBP Elav-like family member 1-A isoform X9"
                     /calculated_mol_wt=55140
     Region          13..94
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          100..180
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          425..516
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..517
                     /gene="LOC117611675"
                     /coded_by="XM_076690521.1:190..1743"
                     /db_xref="GeneID:117611675"
ORIGIN      
        1 mnnnsveqpd pdnikmfvgq vphdmdendl rklfeefgrv hqinilrdki tgshrgccfv
       61 tfytrkaald aqnalhnvkt fsgmrhpiqm kpadsenrne rklfvgmlsk kftendvrnm
      121 fsvygtieec svlrdstgks kacafvtfas kqyainaika lhhsqtmegc ssplvvkfad
      181 tqkekdqkrm qqlqtnlwni agvnmaphyl tndtptlapa slqllqqlqa ttsaatpvaa
      241 saganalssv qhqlllqqhl glgaatpaha aapalpsppe inpanlqgla tlaslsntag
      301 eyantgvspm smqnlvtlaa mtggngnlqv spntlsglan stagmslsvl lgktgntgst
      361 ggslspvtsl alnsltgtpl nglqdslsna ysslqqyagf paftaaaaaa aaaaqkakft
      421 ntdkqiegpe gcnlfiyhlp qefndtdlvt tflpfgnvis akvfidkqtq lskcfgfvsy
      481 dnaasaqaai qamngfqigm krlkvqlkrs kdaskpy
//
LOCUS       XP_034195634             173 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611676 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195634
VERSION     XP_034195634.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339743.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195634.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..173
                     /product="uncharacterized protein LOC117611676 isoform X1"
                     /calculated_mol_wt=20351
     CDS             1..173
                     /gene="LOC117611676"
                     /coded_by="XM_034339743.2:234..755"
                     /db_xref="GeneID:117611676"
ORIGIN      
        1 mdeiqkieil kqeliqaeke ksetfltiis leneiektey kqtsneevan dyeyevdqil
       61 rqsknvgmrr edrwetrkad eyrlkaiqae hhierlydsr eyeerkitql dkkidklkle
      121 insincqnqs stsgygepst skspkmygrc rqlycfaaan gperetstpt srr
//
LOCUS       XP_034195635             162 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611676 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195635
VERSION     XP_034195635.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339744.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195635.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..162
                     /product="uncharacterized protein LOC117611676 isoform X2"
                     /calculated_mol_wt=18984
     CDS             1..162
                     /gene="LOC117611676"
                     /coded_by="XM_034339744.2:235..723"
                     /db_xref="GeneID:117611676"
ORIGIN      
        1 mdeiqkieil kqeliqaeke ksetfltiis leneiektey kqtsneevan dyeyevdqil
       61 rqsknvgmrr edrwetrkad eyrlkaiqae hhierlydsr eyeerkitql dkkidklkle
      121 insincqnqs stsgygepst skspkiwtgt pidcfaqglq cc
//
LOCUS       XP_076546637             364 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305737 [Osmia lignaria lignaria].
ACCESSION   XP_076546637
VERSION     XP_076546637.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690522.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="uncharacterized protein LOC143305737"
                     /calculated_mol_wt=44101
     CDS             1..364
                     /gene="LOC143305737"
                     /coded_by="XM_076690522.1:93..1187"
                     /db_xref="GeneID:143305737"
ORIGIN      
        1 mesqeevlre lrgireevrr lreeikdwrl ergikregke kqesveeiek rrrgleeeeg
       61 kreeeedeae gggcenriev nenavksere easkeeeiev qevekernsg geeekekngr
      121 etieeakekw rrrsegdvde legekewrrr meaegtmrrk eewekiweer leeirkerrk
      181 rsliwkgveg knleerlkyi kkilekelgq evvikrater vgegrrnivl telededrkk
      241 emvwtkngiw drwrvavded lskeerrmkw rikekareei rkgnwvefnn rriwiegeew
      301 kwneraekmg sqqtegqeik kkdrrqgkrt egsrkeeeka kekdqkskev wegeksaggk
      361 gqgr
//
LOCUS       XP_034195530             404 aa            linear   INV 03-SEP-2025
DEFINITION  homeobox protein C15 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195530
VERSION     XP_034195530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339639.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="homeobox protein C15 isoform X1"
                     /calculated_mol_wt=44332
     Region          <252..368
                     /region_name="COG5576"
                     /note="Homeodomain-containing transcription factor
                     [Transcription]"
                     /db_xref="CDD:227863"
     Region          270..326
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..404
                     /gene="C15"
                     /coded_by="XM_034339639.2:726..1940"
                     /db_xref="GeneID:117611626"
ORIGIN      
        1 msvhgdsdad ateeinvdds dsgscsprny thdnrrlpqe hsecsnsppp sqtssrtlqn
       61 esprshpfsi srllgdnrsq nrkspssedl dsdkerkwsw eesnnnvgss mnlddgrrsw
      121 gheieeklse kddddtgsta etqtnssssv hctstadlla pfrlfptgsg liytgggvir
      181 vpahrppgan gtptgalpaq alippwslqa lqhsqqqaaa aglhraslla nlaahplqgh
      241 phlkdrlava gafprrighp yqnrtppkrk kprtsftrlq iaelekrfhk qkylasaera
      301 alakslkmtd aqvktwfqnr rtkwrrqtae ereaerqaan rlmlslqaea lgkvayhpat
      361 vanhppgstv asldrqqppt althpvgqwt spygppqplt epic
//
LOCUS       XP_034195531             347 aa            linear   INV 03-SEP-2025
DEFINITION  homeobox protein C15 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195531
VERSION     XP_034195531.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339640.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..347
                     /product="homeobox protein C15 isoform X2"
                     /calculated_mol_wt=38207
     Region          270..324
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..347
                     /gene="C15"
                     /coded_by="XM_034339640.2:726..1769"
                     /db_xref="GeneID:117611626"
ORIGIN      
        1 msvhgdsdad ateeinvdds dsgscsprny thdnrrlpqe hsecsnsppp sqtssrtlqn
       61 esprshpfsi srllgdnrsq nrkspssedl dsdkerkwsw eesnnnvgss mnlddgrrsw
      121 gheieeklse kddddtgsta etqtnssssv hctstadlla pfrlfptgsg liytgggvir
      181 vpahrppgan gtptgalpaq alippwslqa lqhsqqqaaa aglhraslla nlaahplqgh
      241 phlkdrlava gafprrighp yqnrtppkrk kprtsftrlq iaelekrfhk qkylasaera
      301 alakslkmtd aqvktwfqnr rtkwsllfvs gdkppkkgrl kdkqrig
//
LOCUS       XP_034195532             328 aa            linear   INV 03-SEP-2025
DEFINITION  homeobox protein C15 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195532
VERSION     XP_034195532.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339641.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="homeobox protein C15 isoform X3"
                     /calculated_mol_wt=36056
     Region          270..326
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..328
                     /gene="C15"
                     /coded_by="XM_034339641.2:726..1712"
                     /db_xref="GeneID:117611626"
ORIGIN      
        1 msvhgdsdad ateeinvdds dsgscsprny thdnrrlpqe hsecsnsppp sqtssrtlqn
       61 esprshpfsi srllgdnrsq nrkspssedl dsdkerkwsw eesnnnvgss mnlddgrrsw
      121 gheieeklse kddddtgsta etqtnssssv hctstadlla pfrlfptgsg liytgggvir
      181 vpahrppgan gtptgalpaq alippwslqa lqhsqqqaaa aglhraslla nlaahplqgh
      241 phlkdrlava gafprrighp yqnrtppkrk kprtsftrlq iaelekrfhk qkylasaera
      301 alakslkmtd aqvktwfqnr rtkwrkgg
//
LOCUS       XP_076546638             328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305738 [Osmia lignaria lignaria].
ACCESSION   XP_076546638
VERSION     XP_076546638.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690523.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="uncharacterized protein LOC143305738"
                     /calculated_mol_wt=38405
     Region          116..>216
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     CDS             1..328
                     /gene="LOC143305738"
                     /coded_by="XM_076690523.1:658..1644"
                     /db_xref="GeneID:143305738"
ORIGIN      
        1 msktgetesp kdsgkrgrpa lveqlgkyrs gsqgslldsf krkreeeeae eerrkvekel
       61 ienfqkarri grsppqeate gegteseate gehkdiemdk ldrltelvlt vkrdtqdikk
      121 enesknkeir elkeewkrkq eawelekeei qnriknleik rdkadelier inrierkeea
      181 rekrekrnni tlkgeelprg etpkdtaekv isenlqvkve iedafwmrkg ergsiliakl
      241 rnwqqkkeim mkkgklkgkk icidndltkn erdtqkailg iakaereqga hvkigyrkvr
      301 indrifkwke gvgleeakfw srqssvtl
//
LOCUS       XP_034196112            3718 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196112
VERSION     XP_034196112.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340221.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196112.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3718
                     /product="uncharacterized protein LOC117611843 isoform X1"
                     /calculated_mol_wt=415946
     Region          185..287
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(199,201..202,209,211,222,265)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2776..>3075
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3718
                     /gene="LOC117611843"
                     /coded_by="XM_034340221.2:1479..12635"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mskccscigg yttdtyqpls shisrgsvkl pkdsrdafrs prnqqrklsv rydvvrsvgs
       61 asdpstphrh lagslrgsvn yshnhrhnhl hrhdpplppa yctppppsss syptvqrgca
      121 lhsrldqpvt sstsgmdskk vvqaaplpvp vqvrskndgs gvaasvsgvr rrsgsqglrs
      181 paakrplsap valqgwlhkq gseglmlwkk rwfvlseycl fyykgpeeek llgsillpsy
      241 kvtvckpedk vnrkfafkae hanmrtyhfa adsfesmnqw vnaltlatll qdpnpglgea
      301 avvieiagqq dgersarpsv ssissilnhs addsdsgfhg fqsrddpsha snnnsspnsv
      361 ntasfpnlsg snsnsttnni nnnntnnnva lvnsnnnvne vqpmvngwiq qqtpyiqast
      421 sqqqqqygql mpspqqlqqa mlhqhishqn sqsahqvhqh khvvqqqqsq shlasnvqtv
      481 qpmprkfgqp iyanappkpr rltdgsteys tpspdpdyrk spvspdvtvt skspmsdydr
      541 nsmiygtrms qpsqqslrtd ksglnygytg qvqterrtpd tygrsaakpr snhrgngdye
      601 evygapqlyq rpagpvgytk gaspapipip lyaqqhqqyq qqihspvaas nvpimrqpra
      661 qppprphsad fleyeanrkp qqqlppqcee slnqqrrpqr pkssldivtp sdpandgyfy
      721 seeryaaqmr qsavylhqtp qhhqqqrnqs lsratmpska tgirdgrsed griatlpeas
      781 ctglrrgqre hvhqqhvmph qslqrhtemn sneakmrrsl reksyepssr emlshvedgt
      841 vprriprdye ssmgwgrstt shtsqgahvg qpchaahgaa rrwneqqqfc rsasarlprt
      901 rhpaaldpdd ddygersseq dsrdgerkiq qreesmkrll ewkqrmlqsp ltrkpsgsan
      961 rgrmqnelss yykqqallel aaheasvaen rhsrrredgh rpharskstd grrtvanvsr
     1021 ynsyssddee lgdvrrkrtr rpshagrspr qlgdnrssgi pvqdtvpgsg rqnpnvprcs
     1081 ndhrtspnas vlpnlggipp dagyeevsfp psqrtdrsps yflseahrsv grcdqqelda
     1141 sydeekfkkk psgilktsat ygveqsnksp nsssssgypa eswsgqktvq wttkkerddw
     1201 dsnideskvi kefsyqyikp keevesaede rspgkpeamn lvqcrirsfe snidnsspvk
     1261 evlamqeplk vsplqateah vskldcskks ssiirnfalg eaeaqdskyr nvkeghakqa
     1321 stdsnksvkd lladferksq qvqrqeygrr qevkhrgvfs dseallydtg sdldqrekne
     1381 etgnerdskt dqivpepind ykpyadatkk kgdasfrrkk rspskesttd eipnedldtv
     1441 snpscnrlsv tesllthgdh fsgesgttsp tvkqedinpe ehylpmsprk aildpncddr
     1501 pnpnmmeslf atleheessy vemaqngmth sllapteenr khdsgdystl dtphyefvcv
     1561 sdskmepvym evsqlsekee kdsgklskkr shddsnhsrt dlpdilttlk sdssdaddes
     1621 skdldsidap rhprfslsdt frpasyylga srtmiaelhd ssdselvspp pipttpppld
     1681 dldsldmqes leikkvspqv avmkdnssnr dspkswnkpm gqveshrpps rfsdatisst
     1741 frdsrisles asgsdsvelr tpeeearhrq lktrpvsevc evldsldele slgsrfdgas
     1801 idldqyleel qardafnvdl yakdlsynsi ygfnenmlvv dklqkttchd lsgqvnlgfd
     1861 nleqsmrygn gnkmgsassl psmrvsdvvp pshqhsqsft gdahyenits fsplrgspai
     1921 rsdneqtrdv ghrlqrgsqn nlltashsrd ssysiasaaa sistsmacqr pasaqssmhs
     1981 ptgsislgnh hgnnlptsvl rntspysdqr fqnhsrsasq dsarysmisn hrssssqdsn
     2041 hyptstptvk tnhpsqsylp netraqneqs gapyyysdlq asanavdses skphiprlpq
     2101 lnnqrddasa lnkkndigri vnpisrqmpr qiqvddidea rriaaelrkt tcqllgdnkv
     2161 dlvdkknfye adtlrrvkst dplpdissae isprnlyphg lrdksssqas nretfelthg
     2221 tqtshrrsrs legllddvgl qnlvehrnrs nrvatvqptq vdempqsmgt snvtgsnsnf
     2281 saedpweqds lwreslrrvs lrnarsldnl dspprpsvst sdtksrnkvt rgatyvndsv
     2341 ilrrencmee qqserprvkr rpnkdeerll ddltyeslsm drihaggyvw daeneayrka
     2401 tdedrnhfle egnlppvrsi sesqvsasts aafeldrekl rqwdllssac llqeqqrgsm
     2461 adsgrglpiv erpgdiplpr lttptivpav ndpdvglkvl dnglgnvsta ttttsistst
     2521 tngaqqlkes neaccnraaa asgsgsvigr dplppravst sqlaqrtqtq ppttvstlpl
     2581 srnnashrqp lplqqstpqs vrqsdtdams nsqllraasn gdigtnsvmi nssegkdqrl
     2641 tspgghsnqr lispagalrv vslndhrvss psdsdirihs piernmispi grevdrgggt
     2701 aarqnqrawe cnellhkrsn vtgcklaqpg thglvrvsag ellgrtheel vllliqlrrq
     2761 natiykamet chmeieaqar laeldtprrl enlqkleelk khlmdlekqy ekgkplvnlv
     2821 dnmvklgsly nrntangtss vsgsrhdlah dnsrdhrlef nqkvqeqrll aeerrdwdrl
     2881 spdhgqlqak vqqlykldrl lqeesgtlhs lqqdkeilek algglrhklq gsrsnlaeae
     2941 ryrkqqllle relsrvrvll ahnskkleet vaenarleqd lvvlrqklqa srryagnmtr
     3001 dtsattgple velrrvqqlv gdlqrqrkel svqvrqltek shslvqqirp qppsapqvhq
     3061 pkkrtqnswl etdldsgitl dhgldspssp alsssprgkq nggsylhfss stqikdsspt
     3121 nrqqnhqtfp qspqnhissl spqlreqiqq hqlkqqllkd qmqgkgnlmq anvaplyvnt
     3181 esrmhennkh eenltngtvi pppeyipppp ppplneetll ndnyrqnedn kfaglihnre
     3241 kqeiktvriv kreserrqrd rgdrtgnigi pltnglqapg gakrlcdddl ggsqkfekaq
     3301 lgkvveespm ihaqstvqlt dlddvqfqrs mslprgfggq rqqqpeihqv pvvppprsds
     3361 mhalrsmlar rnkvrfesqd vssdstlspy tdshtssshl pmsspnysts psyspsqqpy
     3421 pgqpirsnqq avyyqqyrhy enpissraen tgdpvspell sptspdvqdr afgstlnvna
     3481 snspqlspvf kseaakqiik emterkvegp rrrqiprekr rhytvssskp vldledtfsk
     3541 mgmgrarddl dmeralrpri napdvvrstl shkelkynes tidqllgtpn kiviperyip
     3601 eqtpeltaee qqhrlkkaea irkmlsettv tategnddni nieksdnlkr kvveekrqrd
     3661 hilqlnqila kqvmekskmv avkalatlpl ntessldded lspvtplply qqrenyys
//
LOCUS       XP_076546639            3675 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546639
VERSION     XP_076546639.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690524.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3675
                     /product="uncharacterized protein LOC117611843 isoform X2"
                     /calculated_mol_wt=411122
     Region          142..244
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(156,158..159,166,168,179,222)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2733..>3032
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3675
                     /gene="LOC117611843"
                     /coded_by="XM_076690524.1:180..11207"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mreavssgmv sfksnnvdsd pstphrhlag slrgsvnysh nhrhnhlhrh dpplppayct
       61 ppppssssyp tvqrgcalhs rldqpvtsst sgmdskkvvq aaplpvpvqv rskndgsgva
      121 asvsgvrrrs gsqglrspaa krplsapval qgwlhkqgse glmlwkkrwf vlseyclfyy
      181 kgpeeekllg sillpsykvt vckpedkvnr kfafkaehan mrtyhfaads fesmnqwvna
      241 ltlatllqdp npglgeaavv ieiagqqdge rsarpsvssi ssilnhsadd sdsgfhgfqs
      301 rddpshasnn nsspnsvnta sfpnlsgsns nsttnninnn ntnnnvalvn snnnvnevqp
      361 mvngwiqqqt pyiqastsqq qqqygqlmps pqqlqqamlh qhishqnsqs ahqvhqhkhv
      421 vqqqqsqshl asnvqtvqpm prkfgqpiya nappkprrlt dgsteystps pdpdyrkspv
      481 spdvtvtsks pmsdydrnsm iygtrmsqps qqslrtdksg lnygytgqvq terrtpdtyg
      541 rsaakprsnh rgngdyeevy gapqlyqrpa gpvgytkgas papipiplya qqhqqyqqqi
      601 hspvaasnvp imrqpraqpp prphsadfle yeanrkpqqq lppqceesln qqrrpqrpks
      661 sldivtpsdp andgyfysee ryaaqmrqsa vylhqtpqhh qqqrnqslsr atmpskatgi
      721 rdgrsedgri atlpeasctg lrrgqrehvh qqhvmphqsl qrhtemnsne akmrrslrek
      781 syepssreml shvedgtvpr riprdyessm gwgrsttsht sqgahvgqpc haahgaarrw
      841 neqqqfcrsa sarlprtrhp aaldpddddy gersseqdsr dgerkiqqre esmkrllewk
      901 qrmlqspltr kpsgsanrgr mqnelssyyk qqallelaah easvaenrhs rrredghrph
      961 arskstdgrr tvanvsryns yssddeelgd vrrkrtrrps hagrsprqlg dnrssgipvq
     1021 dtvpgsgrqn pnvprcsndh rtspnasvlp nlggippdag yeevsfppsq rtdrspsyfl
     1081 seahrsvgrc dqqeldasyd eekfkkkpsg ilktsatygv eqsnkspnss sssgypaesw
     1141 sgqktvqwtt kkerddwdsn ideskvikef syqyikpkee vesaedersp gkpeamnlvq
     1201 crirsfesni dnsspvkevl amqeplkvsp lqateahvsk ldcskksssi irnfalgeae
     1261 aqdskyrnvk eghakqastd snksvkdlla dferksqqvq rqeygrrqev khrgvfsdse
     1321 allydtgsdl dqrekneetg nerdsktdqi vpepindykp yadatkkkgd asfrrkkrsp
     1381 skesttdeip nedldtvsnp scnrlsvtes llthgdhfsg esgttsptvk qedinpeehy
     1441 lpmsprkail dpncddrpnp nmmeslfatl eheessyvem aqngmthsll apteenrkhd
     1501 sgdystldtp hyefvcvsds kmepvymevs qlsekeekds gklskkrshd dsnhsrtdlp
     1561 dilttlksds sdaddesskd ldsidaprhp rfslsdtfrp asyylgasrt miaelhdssd
     1621 selvspppip ttppplddld sldmqeslei kkvspqvavm kdnssnrdsp kswnkpmgqv
     1681 eshrppsrfs datisstfrd srislesasg sdsvelrtpe eearhrqlkt rpvsevcevl
     1741 dsldeleslg srfdgasidl dqyleelqar dafnvdlyak dlsynsiygf nenmlvvdkl
     1801 qkttchdlsg qvnlgfdnle qsmrygngnk mgsasslpsm rvsdvvppsh qhsqsftgda
     1861 hyenitsfsp lrgspairsd neqtrdvghr lqrgsqnnll tashsrdssy siasaaasis
     1921 tsmacqrpas aqssmhsptg sislgnhhgn nlptsvlrnt spysdqrfqn hsrsasqdsa
     1981 rysmisnhrs sssqdsnhyp tstptvktnh psqsylpnet raqneqsgap yyysdlqasa
     2041 navdsesskp hiprlpqlnn qrddasalnk kndigrivnp isrqmprqiq vddidearri
     2101 aaelrkttcq llgdnkvdlv dkknfyeadt lrrvkstdpl pdissaeisp rnlyphglrd
     2161 ksssqasnre tfelthgtqt shrrsrsleg llddvglqnl vehrnrsnrv atvqptqvde
     2221 mpqsmgtsnv tgsnsnfsae dpweqdslwr eslrrvslrn arsldnldsp prpsvstsdt
     2281 ksrnkvtrga tyvndsvilr rencmeeqqs erprvkrrpn kdeerllddl tyeslsmdri
     2341 haggyvwdae neayrkatde drnhfleegn lppvrsises qvsastsaaf eldreklrqw
     2401 dllssacllq eqqrgsmads grglpiverp gdiplprltt ptivpavndp dvglkvldng
     2461 lgnvstattt tsiststtng aqqlkesnea ccnraaaasg sgsvigrdpl ppravstsql
     2521 aqrtqtqppt tvstlplsrn nashrqplpl qqstpqsvrq sdtdamsnsq llraasngdi
     2581 gtnsvminss egkdqrltsp gghsnqrlis pagalrvvsl ndhrvsspsd sdirihspie
     2641 rnmispigre vdrgggtaar qnqrawecne llhkrsnvtg cklaqpgthg lvrvsagell
     2701 grtheelvll liqlrrqnat iykametchm eieaqarlae ldtprrlenl qkleelkkhl
     2761 mdlekqyekg kplvnlvdnm vklgslynrn tangtssvsg srhdlahdns rdhrlefnqk
     2821 vqeqrllaee rrdwdrlspd hgqlqakvqq lykldrllqe esgtlhslqq dkeilekalg
     2881 glrhklqgsr snlaeaeryr kqqlllerel srvrvllahn skkleetvae narleqdlvv
     2941 lrqklqasrr yagnmtrdts attgplevel rrvqqlvgdl qrqrkelsvq vrqltekshs
     3001 lvqqirpqpp sapqvhqpkk rtqnswletd ldsgitldhg ldspsspals ssprgkqngg
     3061 sylhfssstq ikdssptnrq qnhqtfpqsp qnhisslspq lreqiqqhql kqqllkdqmq
     3121 gkgnlmqanv aplyvntesr mhennkheen ltngtvippp eyippppppp lneetllndn
     3181 yrqnednkfa glihnrekqe iktvrivkre serrqrdrgd rtgnigiplt nglqapggak
     3241 rlcdddlggs qkfekaqlgk vveespmiha qstvqltdld dvqfqrsmsl prgfggqrqq
     3301 qpeihqvpvv ppprsdsmha lrsmlarrnk vrfesqdvss dstlspytds htssshlpms
     3361 spnystspsy spsqqpypgq pirsnqqavy yqqyrhyenp issraentgd pvspellspt
     3421 spdvqdrafg stlnvnasns pqlspvfkse aakqiikemt erkvegprrr qiprekrrhy
     3481 tvssskpvld ledtfskmgm grarddldme ralrprinap dvvrstlshk elkynestid
     3541 qllgtpnkiv iperyipeqt peltaeeqqh rlkkaeairk mlsettvtat egnddninie
     3601 ksdnlkrkvv eekrqrdhil qlnqilakqv mekskmvavk alatlplnte sslddedlsp
     3661 vtplplyqqr enyys
//
LOCUS       XP_076546640            3658 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546640
VERSION     XP_076546640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690525.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3658
                     /product="uncharacterized protein LOC117611843 isoform X3"
                     /calculated_mol_wt=409334
     Region          185..287
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(199,201..202,209,211,222,265)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2715..>3015
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3658
                     /gene="LOC117611843"
                     /coded_by="XM_076690525.1:1479..12455"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mskccscigg yttdtyqpls shisrgsvkl pkdsrdafrs prnqqrklsv rydvvrsvgs
       61 asdpstphrh lagslrgsvn yshnhrhnhl hrhdpplppa yctppppsss syptvqrgca
      121 lhsrldqpvt sstsgmdskk vvqaaplpvp vqvrskndgs gvaasvsgvr rrsgsqglrs
      181 paakrplsap valqgwlhkq gseglmlwkk rwfvlseycl fyykgpeeek llgsillpsy
      241 kvtvckpedk vnrkfafkae hanmrtyhfa adsfesmnqw vnaltlatll qdpnpglgea
      301 avvieiagqq dgersarpsv ssissilnhs addsdsgfhg fqsrddpsha snnnsspnsv
      361 ntasfpnlsg snsnsttnni nnnntnnnva lvnsnnnvne vqpmvngwiq qqtpyiqast
      421 sqqqqqygql mpspqqlqqa mlhqhishqn sqsahqvhqh khvvqqqqsq shlasnvqtv
      481 qpmprkfgqp iyanappkpr rltdgsteys tpspdpdyrk spvspdvtvt skspmsdydr
      541 nsmiygtrms qpsqqslrtd ksglnygytg qvqterrtpd tygrsaakpr snhrgngdye
      601 evygapqlyq rpagpvgytk gaspapipip lyaqqhqqyq qqihspvaas nvpimrqpra
      661 qppprphsad fleyeanrkp qqqlppqcee slnqqrrpqr pkssldivtp sdpandgyfy
      721 seeryaaqmr qsavylhqtp qhhqqqrnqs lsratmpska tgirdgrsed griatlpeas
      781 ctglrrgqre hvhqqhvmph qslqrhtemn sneakmrrsl reksyepssr emlshvedgt
      841 vprriprdye ssmgwgrstt shtsqgahvg qpchaahgaa rrwneqqqfc rsasarlprt
      901 rhpaaldpdd ddygersseq dsrdgerkiq qreesmkrll ewkqrmlqsp ltrkpsgsan
      961 rgrmqnelss yykqqallel aaheasvaen rhsrrredgh rpharskstd grrtvanvsr
     1021 ynsyssddee lgdvrrkrtr rpshagrspr qlgdnrssgi pvqdtvpgsg rqnpnvprcs
     1081 ndhrtspnas vlpnlggipp dagyeevsfp psqrtdrsps yflseahrsv grcdqqelda
     1141 sydeekfkkk psgilktsat ygveqsnksp nsssssgypa eswsgqktvq wttkkerddw
     1201 dsnideskvi kefsyqyikp keevesaede rspgkpeamn lvqcrirsfe snidnsspvk
     1261 evlamqeplk vsplqateah vskldcskks ssiirnfalg eaeaqdskyr nvkeghakqa
     1321 stdsnksvkd lladferksq qvqrqeygrr qevkhrgvfs dseallydtg sdldqrekne
     1381 etgnerdskt dqivpepind ykpyadatkk kgdasfrrkk rspskesttd eipnedldtv
     1441 snpscnrlsv tesllthgdh fsgesgttsp tvkqedinpe ehylpmsprk aildpncddr
     1501 pnpnmmeslf atleheessy vemaqngmth sllapteenr khdsgdystl dtphyefvcv
     1561 sdskmepvym evsqlsekee kdsgklskkr shddsnhsrt dlpdilttlk sdssdaddes
     1621 skdldsidap rhprfslsdt frpasyylga srtmiaelhd ssdselvspp pipttpppld
     1681 dldsldmqes leikkvspqv avmkdnssnr dspkswnkpm gqveshrpps rfsdatisst
     1741 frdsrisles asgsdsvelr tpeeearhrq lktrpvsevc evldsldele slgsrfdgas
     1801 idldqyleel qardafnvdl yakdlsynsi ygfnenmlvv dklqkttchd lsgqvnlgfd
     1861 nleqsmrygn gnkmgsassl psmrvsdvvp pshqhsqsft gdahyenits fsplrgspai
     1921 rsdneqtrdv ghrlqrgsqn nlltashsrd ssysiasaaa sistsmacqr pasaqssmhs
     1981 ptgsislgnh hgnnlptsvl rntspysdqr fqnhsrsasq dsarysmisn hrssssqdsn
     2041 hyptstptvk tnhpsqsylp netraqneqs gapyyysdlq asanavdses skphiprlpq
     2101 lnnqrddasa lnkkndigri vnpisrqmpr qiqvddidea rriaaelrkt tcqllgdnkv
     2161 dlvdkknfye adtlrrvkst dplpdissae isprnlyphg lrdksssqas nretfelthg
     2221 tqtshrrsrs legllddvgl qnlvehrnrs nrvatvqptq vdempqsmgt snvtgsnsnf
     2281 saedpweqds lwreslrrvs lrnarsldnl dspprpsvst sdtksrnkvt rgatyvndsv
     2341 ilrrencmee qqserprvkr rpnkdeerll ddltyeslsm drihaggyvw daeneayrka
     2401 tdedrnhfle egnlppvrsi sesqvsasts aafeldrekl rqwdllssac llqeqqrgsm
     2461 adsgrglpiv erpgdiplpr lttptivpav ndpdvglkvl dnglgnvsta ttttsistst
     2521 tngaqqlkes neaccnraaa asgsgsvigr dplppravst sqlaqrtqtq ppttvstlpl
     2581 srnnashrqp lplqqstpqs vrqsdtdams nsqllraasn gdigtnsvmi nssegkdqrl
     2641 tspgghsnqr lispagalrv vslndhrvss psdsdirihs piernmispi grevdrgggt
     2701 aarqnqrawe cnellhkrar laeldtprrl enlqkleelk khlmdlekqy ekgkplvnlv
     2761 dnmvklgsly nrntangtss vsgsrhdlah dnsrdhrlef nqkvqeqrll aeerrdwdrl
     2821 spdhgqlqak vqqlykldrl lqeesgtlhs lqqdkeilek algglrhklq gsrsnlaeae
     2881 ryrkqqllle relsrvrvll ahnskkleet vaenarleqd lvvlrqklqa srryagnmtr
     2941 dtsattgple velrrvqqlv gdlqrqrkel svqvrqltek shslvqqirp qppsapqvhq
     3001 pkkrtqnswl etdldsgitl dhgldspssp alsssprgkq nggsylhfss stqikdsspt
     3061 nrqqnhqtfp qspqnhissl spqlreqiqq hqlkqqllkd qmqgkgnlmq anvaplyvnt
     3121 esrmhennkh eenltngtvi pppeyipppp ppplneetll ndnyrqnedn kfaglihnre
     3181 kqeiktvriv kreserrqrd rgdrtgnigi pltnglqapg gakrlcdddl ggsqkfekaq
     3241 lgkvveespm ihaqstvqlt dlddvqfqrs mslprgfggq rqqqpeihqv pvvppprsds
     3301 mhalrsmlar rnkvrfesqd vssdstlspy tdshtssshl pmsspnysts psyspsqqpy
     3361 pgqpirsnqq avyyqqyrhy enpissraen tgdpvspell sptspdvqdr afgstlnvna
     3421 snspqlspvf kseaakqiik emterkvegp rrrqiprekr rhytvssskp vldledtfsk
     3481 mgmgrarddl dmeralrpri napdvvrstl shkelkynes tidqllgtpn kiviperyip
     3541 eqtpeltaee qqhrlkkaea irkmlsettv tategnddni nieksdnlkr kvveekrqrd
     3601 hilqlnqila kqvmekskmv avkalatlpl ntessldded lspvtplply qqrenyys
//
LOCUS       XP_076546641            3605 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546641
VERSION     XP_076546641.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690526.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3605
                     /product="uncharacterized protein LOC117611843 isoform X4"
                     /calculated_mol_wt=403715
     Region          72..174
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(86,88..89,96,98,109,152)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2663..>2972
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3605
                     /gene="LOC117611843"
                     /coded_by="XM_076690526.1:654..11471"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mftclwnffd wldqpvtsst sgmdskkvvq aaplpvpvqv rskndgsgva asvsgvrrrs
       61 gsqglrspaa krplsapval qgwlhkqgse glmlwkkrwf vlseyclfyy kgpeeekllg
      121 sillpsykvt vckpedkvnr kfafkaehan mrtyhfaads fesmnqwvna ltlatllqdp
      181 npglgeaavv ieiagqqdge rsarpsvssi ssilnhsadd sdsgfhgfqs rddpshasnn
      241 nsspnsvnta sfpnlsgsns nsttnninnn ntnnnvalvn snnnvnevqp mvngwiqqqt
      301 pyiqastsqq qqqygqlmps pqqlqqamlh qhishqnsqs ahqvhqhkhv vqqqqsqshl
      361 asnvqtvqpm prkfgqpiya nappkprrlt dgsteystps pdpdyrkspv spdvtvtsks
      421 pmsdydrnsm iygtrmsqps qqslrtdksg lnygytgqvq terrtpdtyg rsaakprsnh
      481 rgngdyeevy gapqlyqrpa gpvgytkgas papipiplya qqhqqyqqqi hspvaasnvp
      541 imrqpraqpp prphsadfle yeanrkpqqq lppqceesln qqrrpqrpks sldivtpsdp
      601 andgyfysee ryaaqmrqsa vylhqtpqhh qqqrnqslsr atmpskatgi rdgrsedgri
      661 atlpeasctg lrrgqrehvh qqhvmphqsl qrhtemnsne akmrrslrek syepssreml
      721 shvedgtvpr riprdyessm gwgrsttsht sqgahvgqpc haahgaarrw neqqqfcrsa
      781 sarlprtrhp aaldpddddy gersseqdsr dgerkiqqre esmkrllewk qrmlqspltr
      841 kpsgsanrgr mqnelssyyk qqallelaah easvaenrhs rrredghrph arskstdgrr
      901 tvanvsryns yssddeelgd vrrkrtrrps hagrsprqlg dnrssgipvq dtvpgsgrqn
      961 pnvprcsndh rtspnasvlp nlggippdag yeevsfppsq rtdrspsyfl seahrsvgrc
     1021 dqqeldasyd eekfkkkpsg ilktsatygv eqsnkspnss sssgypaesw sgqktvqwtt
     1081 kkerddwdsn ideskvikef syqyikpkee vesaedersp gkpeamnlvq crirsfesni
     1141 dnsspvkevl amqeplkvsp lqateahvsk ldcskksssi irnfalgeae aqdskyrnvk
     1201 eghakqastd snksvkdlla dferksqqvq rqeygrrqev khrgvfsdse allydtgsdl
     1261 dqrekneetg nerdsktdqi vpepindykp yadatkkkgd asfrrkkrsp skesttdeip
     1321 nedldtvsnp scnrlsvtes llthgdhfsg esgttsptvk qedinpeehy lpmsprkail
     1381 dpncddrpnp nmmeslfatl eheessyvem aqngmthsll apteenrkhd sgdystldtp
     1441 hyefvcvsds kmepvymevs qlsekeekds gklskkrshd dsnhsrtdlp dilttlksds
     1501 sdaddesskd ldsidaprhp rfslsdtfrp asyylgasrt miaelhdssd selvspppip
     1561 ttppplddld sldmqeslei kkvspqvavm kdnssnrdsp kswnkpmgqv eshrppsrfs
     1621 datisstfrd srislesasg sdsvelrtpe eearhrqlkt rpvsevcevl dsldeleslg
     1681 srfdgasidl dqyleelqar dafnvdlyak dlsynsiygf nenmlvvdkl qkttchdlsg
     1741 qvnlgfdnle qsmrygngnk mgsasslpsm rvsdvvppsh qhsqsftgda hyenitsfsp
     1801 lrgspairsd neqtrdvghr lqrgsqnnll tashsrdssy siasaaasis tsmacqrpas
     1861 aqssmhsptg sislgnhhgn nlptsvlrnt spysdqrfqn hsrsasqdsa rysmisnhrs
     1921 sssqdsnhyp tstptvktnh psqsylpnet raqneqsgap yyysdlqasa navdsesskp
     1981 hiprlpqlnn qrddasalnk kndigrivnp isrqmprqiq vddidearri aaelrkttcq
     2041 llgdnkvdlv dkknfyeadt lrrvkstdpl pdissaeisp rnlyphglrd ksssqasnre
     2101 tfelthgtqt shrrsrsleg llddvglqnl vehrnrsnrv atvqptqvde mpqsmgtsnv
     2161 tgsnsnfsae dpweqdslwr eslrrvslrn arsldnldsp prpsvstsdt ksrnkvtrga
     2221 tyvndsvilr rencmeeqqs erprvkrrpn kdeerllddl tyeslsmdri haggyvwdae
     2281 neayrkatde drnhfleegn lppvrsises qvsastsaaf eldreklrqw dllssacllq
     2341 eqqrgsmads grglpiverp gdiplprltt ptivpavndp dvglkvldng lgnvstattt
     2401 tsiststtng aqqlkesnea ccnraaaasg sgsvigrdpl ppravstsql aqrtqtqppt
     2461 tvstlplsrn nashrqplpl qqstpqsvrq sdtdamsnsq llraasngdi gtnsvminss
     2521 egkdqrltsp gghsnqrlis pagalrvvsl ndhrvsspsd sdirihspie rnmispigre
     2581 vdrgggtaar qnqrawecne llhkrsnvtg cklaqpgthg lvrvsagell grtheelvll
     2641 liqlrrqnat iykametchm eieaqarlae ldtprrlenl qkleelkkhl mdlekqyekg
     2701 kplvnlvdnm vklgslynrn tangtssvsg srhdlahdns rdhrlefnqk vqeqrllaee
     2761 rrdwdrlspd hgqlqakvqq lykldrllqe esgtlhslqq dkeilekalg glrhklqgsr
     2821 snlaeaeryr kqqlllerel srvrvllahn skkleetvae narleqdlvv lrqklqasrr
     2881 yagnmtrdts attgplevel rrvqqlvgdl qrqrkelsvq vrqltekshs lvqqirpqpp
     2941 sapqvhqpkk rtqnswletd ldsgitldhg ldspsspals ssprgkqngg sylhfssstq
     3001 ikdssptnrq qnhqtfpqsp qnhisslspq lreqiqqhql kqqllkdqmq gkgnlmqanv
     3061 aplyvntesr mhennkheen ltngtvippp eyippppppp lneetllndn yrqnednkfa
     3121 glihnrekqe iktvrivkre serrqrdrgd rtgnigiplt nglqapggak rlcdddlggs
     3181 qkfekaqlgk vveespmiha qstvqltdld dvqfqrsmsl prgfggqrqq qpeihqvpvv
     3241 ppprsdsmha lrsmlarrnk vrfesqdvss dstlspytds htssshlpms spnystspsy
     3301 spsqqpypgq pirsnqqavy yqqyrhyenp issraentgd pvspellspt spdvqdrafg
     3361 stlnvnasns pqlspvfkse aakqiikemt erkvegprrr qiprekrrhy tvssskpvld
     3421 ledtfskmgm grarddldme ralrprinap dvvrstlshk elkynestid qllgtpnkiv
     3481 iperyipeqt peltaeeqqh rlkkaeairk mlsettvtat egnddninie ksdnlkrkvv
     3541 eekrqrdhil qlnqilakqv mekskmvavk alatlplnte sslddedlsp vtplplyqqr
     3601 enyys
//
LOCUS       XP_076546642            3583 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546642
VERSION     XP_076546642.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690527.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3583
                     /product="uncharacterized protein LOC117611843 isoform X5"
                     /calculated_mol_wt=401150
     Region          50..152
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(64,66..67,74,76,87,130)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2641..>2940
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3583
                     /gene="LOC117611843"
                     /coded_by="XM_076690527.1:2934..13685"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mdskkvvqaa plpvpvqvrs kndgsgvaas vsgvrrrsgs qglrspaakr plsapvalqg
       61 wlhkqgsegl mlwkkrwfvl seyclfyykg peeekllgsi llpsykvtvc kpedkvnrkf
      121 afkaehanmr tyhfaadsfe smnqwvnalt latllqdpnp glgeaavvie iagqqdgers
      181 arpsvssiss ilnhsaddsd sgfhgfqsrd dpshasnnns spnsvntasf pnlsgsnsns
      241 ttnninnnnt nnnvalvnsn nnvnevqpmv ngwiqqqtpy iqastsqqqq qygqlmpspq
      301 qlqqamlhqh ishqnsqsah qvhqhkhvvq qqqsqshlas nvqtvqpmpr kfgqpiyana
      361 ppkprrltdg steystpspd pdyrkspvsp dvtvtskspm sdydrnsmiy gtrmsqpsqq
      421 slrtdksgln ygytgqvqte rrtpdtygrs aakprsnhrg ngdyeevyga pqlyqrpagp
      481 vgytkgaspa pipiplyaqq hqqyqqqihs pvaasnvpim rqpraqpppr phsadfleye
      541 anrkpqqqlp pqceeslnqq rrpqrpkssl divtpsdpan dgyfyseery aaqmrqsavy
      601 lhqtpqhhqq qrnqslsrat mpskatgird grsedgriat lpeasctglr rgqrehvhqq
      661 hvmphqslqr htemnsneak mrrslreksy epssremlsh vedgtvprri prdyessmgw
      721 grsttshtsq gahvgqpcha ahgaarrwne qqqfcrsasa rlprtrhpaa ldpddddyge
      781 rsseqdsrdg erkiqqrees mkrllewkqr mlqspltrkp sgsanrgrmq nelssyykqq
      841 allelaahea svaenrhsrr redghrphar skstdgrrtv anvsrynsys sddeelgdvr
      901 rkrtrrpsha grsprqlgdn rssgipvqdt vpgsgrqnpn vprcsndhrt spnasvlpnl
      961 ggippdagye evsfppsqrt drspsyflse ahrsvgrcdq qeldasydee kfkkkpsgil
     1021 ktsatygveq snkspnssss sgypaeswsg qktvqwttkk erddwdsnid eskvikefsy
     1081 qyikpkeeve saederspgk peamnlvqcr irsfesnidn sspvkevlam qeplkvsplq
     1141 ateahvskld cskksssiir nfalgeaeaq dskyrnvkeg hakqastdsn ksvkdlladf
     1201 erksqqvqrq eygrrqevkh rgvfsdseal lydtgsdldq rekneetgne rdsktdqivp
     1261 epindykpya datkkkgdas frrkkrspsk esttdeipne dldtvsnpsc nrlsvtesll
     1321 thgdhfsges gttsptvkqe dinpeehylp msprkaildp ncddrpnpnm meslfatleh
     1381 eessyvemaq ngmthsllap teenrkhdsg dystldtphy efvcvsdskm epvymevsql
     1441 sekeekdsgk lskkrshdds nhsrtdlpdi lttlksdssd addesskdld sidaprhprf
     1501 slsdtfrpas yylgasrtmi aelhdssdse lvspppiptt ppplddldsl dmqesleikk
     1561 vspqvavmkd nssnrdspks wnkpmgqves hrppsrfsda tisstfrdsr islesasgsd
     1621 svelrtpeee arhrqlktrp vsevcevlds ldeleslgsr fdgasidldq yleelqarda
     1681 fnvdlyakdl synsiygfne nmlvvdklqk ttchdlsgqv nlgfdnleqs mrygngnkmg
     1741 sasslpsmrv sdvvppshqh sqsftgdahy enitsfsplr gspairsdne qtrdvghrlq
     1801 rgsqnnllta shsrdssysi asaaasists macqrpasaq ssmhsptgsi slgnhhgnnl
     1861 ptsvlrntsp ysdqrfqnhs rsasqdsary smisnhrsss sqdsnhypts tptvktnhps
     1921 qsylpnetra qneqsgapyy ysdlqasana vdsesskphi prlpqlnnqr ddasalnkkn
     1981 digrivnpis rqmprqiqvd didearriaa elrkttcqll gdnkvdlvdk knfyeadtlr
     2041 rvkstdplpd issaeisprn lyphglrdks ssqasnretf elthgtqtsh rrsrslegll
     2101 ddvglqnlve hrnrsnrvat vqptqvdemp qsmgtsnvtg snsnfsaedp weqdslwres
     2161 lrrvslrnar sldnldsppr psvstsdtks rnkvtrgaty vndsvilrre ncmeeqqser
     2221 prvkrrpnkd eerllddlty eslsmdriha ggyvwdaene ayrkatdedr nhfleegnlp
     2281 pvrsisesqv sastsaafel dreklrqwdl lssacllqeq qrgsmadsgr glpiverpgd
     2341 iplprlttpt ivpavndpdv glkvldnglg nvstatttts iststtngaq qlkesneacc
     2401 nraaaasgsg svigrdplpp ravstsqlaq rtqtqppttv stlplsrnna shrqplplqq
     2461 stpqsvrqsd tdamsnsqll raasngdigt nsvminsseg kdqrltspgg hsnqrlispa
     2521 galrvvslnd hrvsspsdsd irihspiern mispigrevd rgggtaarqn qrawecnell
     2581 hkrsnvtgck laqpgthglv rvsagellgr theelvllli qlrrqnatiy kametchmei
     2641 eaqarlaeld tprrlenlqk leelkkhlmd lekqyekgkp lvnlvdnmvk lgslynrnta
     2701 ngtssvsgsr hdlahdnsrd hrlefnqkvq eqrllaeerr dwdrlspdhg qlqakvqqly
     2761 kldrllqees gtlhslqqdk eilekalggl rhklqgsrsn laeaeryrkq qlllerelsr
     2821 vrvllahnsk kleetvaena rleqdlvvlr qklqasrrya gnmtrdtsat tgplevelrr
     2881 vqqlvgdlqr qrkelsvqvr qltekshslv qqirpqppsa pqvhqpkkrt qnswletdld
     2941 sgitldhgld spsspalsss prgkqnggsy lhfssstqik dssptnrqqn hqtfpqspqn
     3001 hisslspqlr eqiqqhqlkq qllkdqmqgk gnlmqanvap lyvntesrmh ennkheenlt
     3061 ngtvipppey ipppppppln eetllndnyr qnednkfagl ihnrekqeik tvrivkrese
     3121 rrqrdrgdrt gnigipltng lqapggakrl cdddlggsqk fekaqlgkvv eespmihaqs
     3181 tvqltdlddv qfqrsmslpr gfggqrqqqp eihqvpvvpp prsdsmhalr smlarrnkvr
     3241 fesqdvssds tlspytdsht ssshlpmssp nystspsysp sqqpypgqpi rsnqqavyyq
     3301 qyrhyenpis sraentgdpv spellsptsp dvqdrafgst lnvnasnspq lspvfkseaa
     3361 kqiikemter kvegprrrqi prekrrhytv ssskpvldle dtfskmgmgr arddldmera
     3421 lrprinapdv vrstlshkel kynestidql lgtpnkivip eryipeqtpe ltaeeqqhrl
     3481 kkaeairkml settvtateg nddninieks dnlkrkvvee krqrdhilql nqilakqvme
     3541 kskmvavkal atlplntess lddedlspvt plplyqqren yys
//
LOCUS       XP_076546643            3583 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546643
VERSION     XP_076546643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690528.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3583
                     /product="uncharacterized protein LOC117611843 isoform X5"
                     /calculated_mol_wt=401150
     Region          50..152
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(64,66..67,74,76,87,130)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2641..>2940
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3583
                     /gene="LOC117611843"
                     /coded_by="XM_076690528.1:2864..13615"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mdskkvvqaa plpvpvqvrs kndgsgvaas vsgvrrrsgs qglrspaakr plsapvalqg
       61 wlhkqgsegl mlwkkrwfvl seyclfyykg peeekllgsi llpsykvtvc kpedkvnrkf
      121 afkaehanmr tyhfaadsfe smnqwvnalt latllqdpnp glgeaavvie iagqqdgers
      181 arpsvssiss ilnhsaddsd sgfhgfqsrd dpshasnnns spnsvntasf pnlsgsnsns
      241 ttnninnnnt nnnvalvnsn nnvnevqpmv ngwiqqqtpy iqastsqqqq qygqlmpspq
      301 qlqqamlhqh ishqnsqsah qvhqhkhvvq qqqsqshlas nvqtvqpmpr kfgqpiyana
      361 ppkprrltdg steystpspd pdyrkspvsp dvtvtskspm sdydrnsmiy gtrmsqpsqq
      421 slrtdksgln ygytgqvqte rrtpdtygrs aakprsnhrg ngdyeevyga pqlyqrpagp
      481 vgytkgaspa pipiplyaqq hqqyqqqihs pvaasnvpim rqpraqpppr phsadfleye
      541 anrkpqqqlp pqceeslnqq rrpqrpkssl divtpsdpan dgyfyseery aaqmrqsavy
      601 lhqtpqhhqq qrnqslsrat mpskatgird grsedgriat lpeasctglr rgqrehvhqq
      661 hvmphqslqr htemnsneak mrrslreksy epssremlsh vedgtvprri prdyessmgw
      721 grsttshtsq gahvgqpcha ahgaarrwne qqqfcrsasa rlprtrhpaa ldpddddyge
      781 rsseqdsrdg erkiqqrees mkrllewkqr mlqspltrkp sgsanrgrmq nelssyykqq
      841 allelaahea svaenrhsrr redghrphar skstdgrrtv anvsrynsys sddeelgdvr
      901 rkrtrrpsha grsprqlgdn rssgipvqdt vpgsgrqnpn vprcsndhrt spnasvlpnl
      961 ggippdagye evsfppsqrt drspsyflse ahrsvgrcdq qeldasydee kfkkkpsgil
     1021 ktsatygveq snkspnssss sgypaeswsg qktvqwttkk erddwdsnid eskvikefsy
     1081 qyikpkeeve saederspgk peamnlvqcr irsfesnidn sspvkevlam qeplkvsplq
     1141 ateahvskld cskksssiir nfalgeaeaq dskyrnvkeg hakqastdsn ksvkdlladf
     1201 erksqqvqrq eygrrqevkh rgvfsdseal lydtgsdldq rekneetgne rdsktdqivp
     1261 epindykpya datkkkgdas frrkkrspsk esttdeipne dldtvsnpsc nrlsvtesll
     1321 thgdhfsges gttsptvkqe dinpeehylp msprkaildp ncddrpnpnm meslfatleh
     1381 eessyvemaq ngmthsllap teenrkhdsg dystldtphy efvcvsdskm epvymevsql
     1441 sekeekdsgk lskkrshdds nhsrtdlpdi lttlksdssd addesskdld sidaprhprf
     1501 slsdtfrpas yylgasrtmi aelhdssdse lvspppiptt ppplddldsl dmqesleikk
     1561 vspqvavmkd nssnrdspks wnkpmgqves hrppsrfsda tisstfrdsr islesasgsd
     1621 svelrtpeee arhrqlktrp vsevcevlds ldeleslgsr fdgasidldq yleelqarda
     1681 fnvdlyakdl synsiygfne nmlvvdklqk ttchdlsgqv nlgfdnleqs mrygngnkmg
     1741 sasslpsmrv sdvvppshqh sqsftgdahy enitsfsplr gspairsdne qtrdvghrlq
     1801 rgsqnnllta shsrdssysi asaaasists macqrpasaq ssmhsptgsi slgnhhgnnl
     1861 ptsvlrntsp ysdqrfqnhs rsasqdsary smisnhrsss sqdsnhypts tptvktnhps
     1921 qsylpnetra qneqsgapyy ysdlqasana vdsesskphi prlpqlnnqr ddasalnkkn
     1981 digrivnpis rqmprqiqvd didearriaa elrkttcqll gdnkvdlvdk knfyeadtlr
     2041 rvkstdplpd issaeisprn lyphglrdks ssqasnretf elthgtqtsh rrsrslegll
     2101 ddvglqnlve hrnrsnrvat vqptqvdemp qsmgtsnvtg snsnfsaedp weqdslwres
     2161 lrrvslrnar sldnldsppr psvstsdtks rnkvtrgaty vndsvilrre ncmeeqqser
     2221 prvkrrpnkd eerllddlty eslsmdriha ggyvwdaene ayrkatdedr nhfleegnlp
     2281 pvrsisesqv sastsaafel dreklrqwdl lssacllqeq qrgsmadsgr glpiverpgd
     2341 iplprlttpt ivpavndpdv glkvldnglg nvstatttts iststtngaq qlkesneacc
     2401 nraaaasgsg svigrdplpp ravstsqlaq rtqtqppttv stlplsrnna shrqplplqq
     2461 stpqsvrqsd tdamsnsqll raasngdigt nsvminsseg kdqrltspgg hsnqrlispa
     2521 galrvvslnd hrvsspsdsd irihspiern mispigrevd rgggtaarqn qrawecnell
     2581 hkrsnvtgck laqpgthglv rvsagellgr theelvllli qlrrqnatiy kametchmei
     2641 eaqarlaeld tprrlenlqk leelkkhlmd lekqyekgkp lvnlvdnmvk lgslynrnta
     2701 ngtssvsgsr hdlahdnsrd hrlefnqkvq eqrllaeerr dwdrlspdhg qlqakvqqly
     2761 kldrllqees gtlhslqqdk eilekalggl rhklqgsrsn laeaeryrkq qlllerelsr
     2821 vrvllahnsk kleetvaena rleqdlvvlr qklqasrrya gnmtrdtsat tgplevelrr
     2881 vqqlvgdlqr qrkelsvqvr qltekshslv qqirpqppsa pqvhqpkkrt qnswletdld
     2941 sgitldhgld spsspalsss prgkqnggsy lhfssstqik dssptnrqqn hqtfpqspqn
     3001 hisslspqlr eqiqqhqlkq qllkdqmqgk gnlmqanvap lyvntesrmh ennkheenlt
     3061 ngtvipppey ipppppppln eetllndnyr qnednkfagl ihnrekqeik tvrivkrese
     3121 rrqrdrgdrt gnigipltng lqapggakrl cdddlggsqk fekaqlgkvv eespmihaqs
     3181 tvqltdlddv qfqrsmslpr gfggqrqqqp eihqvpvvpp prsdsmhalr smlarrnkvr
     3241 fesqdvssds tlspytdsht ssshlpmssp nystspsysp sqqpypgqpi rsnqqavyyq
     3301 qyrhyenpis sraentgdpv spellsptsp dvqdrafgst lnvnasnspq lspvfkseaa
     3361 kqiikemter kvegprrrqi prekrrhytv ssskpvldle dtfskmgmgr arddldmera
     3421 lrprinapdv vrstlshkel kynestidql lgtpnkivip eryipeqtpe ltaeeqqhrl
     3481 kkaeairkml settvtateg nddninieks dnlkrkvvee krqrdhilql nqilakqvme
     3541 kskmvavkal atlplntess lddedlspvt plplyqqren yys
//
LOCUS       XP_076546644            3583 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546644
VERSION     XP_076546644.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690529.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3583
                     /product="uncharacterized protein LOC117611843 isoform X5"
                     /calculated_mol_wt=401150
     Region          50..152
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(64,66..67,74,76,87,130)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2641..>2940
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3583
                     /gene="LOC117611843"
                     /coded_by="XM_076690529.1:2514..13265"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mdskkvvqaa plpvpvqvrs kndgsgvaas vsgvrrrsgs qglrspaakr plsapvalqg
       61 wlhkqgsegl mlwkkrwfvl seyclfyykg peeekllgsi llpsykvtvc kpedkvnrkf
      121 afkaehanmr tyhfaadsfe smnqwvnalt latllqdpnp glgeaavvie iagqqdgers
      181 arpsvssiss ilnhsaddsd sgfhgfqsrd dpshasnnns spnsvntasf pnlsgsnsns
      241 ttnninnnnt nnnvalvnsn nnvnevqpmv ngwiqqqtpy iqastsqqqq qygqlmpspq
      301 qlqqamlhqh ishqnsqsah qvhqhkhvvq qqqsqshlas nvqtvqpmpr kfgqpiyana
      361 ppkprrltdg steystpspd pdyrkspvsp dvtvtskspm sdydrnsmiy gtrmsqpsqq
      421 slrtdksgln ygytgqvqte rrtpdtygrs aakprsnhrg ngdyeevyga pqlyqrpagp
      481 vgytkgaspa pipiplyaqq hqqyqqqihs pvaasnvpim rqpraqpppr phsadfleye
      541 anrkpqqqlp pqceeslnqq rrpqrpkssl divtpsdpan dgyfyseery aaqmrqsavy
      601 lhqtpqhhqq qrnqslsrat mpskatgird grsedgriat lpeasctglr rgqrehvhqq
      661 hvmphqslqr htemnsneak mrrslreksy epssremlsh vedgtvprri prdyessmgw
      721 grsttshtsq gahvgqpcha ahgaarrwne qqqfcrsasa rlprtrhpaa ldpddddyge
      781 rsseqdsrdg erkiqqrees mkrllewkqr mlqspltrkp sgsanrgrmq nelssyykqq
      841 allelaahea svaenrhsrr redghrphar skstdgrrtv anvsrynsys sddeelgdvr
      901 rkrtrrpsha grsprqlgdn rssgipvqdt vpgsgrqnpn vprcsndhrt spnasvlpnl
      961 ggippdagye evsfppsqrt drspsyflse ahrsvgrcdq qeldasydee kfkkkpsgil
     1021 ktsatygveq snkspnssss sgypaeswsg qktvqwttkk erddwdsnid eskvikefsy
     1081 qyikpkeeve saederspgk peamnlvqcr irsfesnidn sspvkevlam qeplkvsplq
     1141 ateahvskld cskksssiir nfalgeaeaq dskyrnvkeg hakqastdsn ksvkdlladf
     1201 erksqqvqrq eygrrqevkh rgvfsdseal lydtgsdldq rekneetgne rdsktdqivp
     1261 epindykpya datkkkgdas frrkkrspsk esttdeipne dldtvsnpsc nrlsvtesll
     1321 thgdhfsges gttsptvkqe dinpeehylp msprkaildp ncddrpnpnm meslfatleh
     1381 eessyvemaq ngmthsllap teenrkhdsg dystldtphy efvcvsdskm epvymevsql
     1441 sekeekdsgk lskkrshdds nhsrtdlpdi lttlksdssd addesskdld sidaprhprf
     1501 slsdtfrpas yylgasrtmi aelhdssdse lvspppiptt ppplddldsl dmqesleikk
     1561 vspqvavmkd nssnrdspks wnkpmgqves hrppsrfsda tisstfrdsr islesasgsd
     1621 svelrtpeee arhrqlktrp vsevcevlds ldeleslgsr fdgasidldq yleelqarda
     1681 fnvdlyakdl synsiygfne nmlvvdklqk ttchdlsgqv nlgfdnleqs mrygngnkmg
     1741 sasslpsmrv sdvvppshqh sqsftgdahy enitsfsplr gspairsdne qtrdvghrlq
     1801 rgsqnnllta shsrdssysi asaaasists macqrpasaq ssmhsptgsi slgnhhgnnl
     1861 ptsvlrntsp ysdqrfqnhs rsasqdsary smisnhrsss sqdsnhypts tptvktnhps
     1921 qsylpnetra qneqsgapyy ysdlqasana vdsesskphi prlpqlnnqr ddasalnkkn
     1981 digrivnpis rqmprqiqvd didearriaa elrkttcqll gdnkvdlvdk knfyeadtlr
     2041 rvkstdplpd issaeisprn lyphglrdks ssqasnretf elthgtqtsh rrsrslegll
     2101 ddvglqnlve hrnrsnrvat vqptqvdemp qsmgtsnvtg snsnfsaedp weqdslwres
     2161 lrrvslrnar sldnldsppr psvstsdtks rnkvtrgaty vndsvilrre ncmeeqqser
     2221 prvkrrpnkd eerllddlty eslsmdriha ggyvwdaene ayrkatdedr nhfleegnlp
     2281 pvrsisesqv sastsaafel dreklrqwdl lssacllqeq qrgsmadsgr glpiverpgd
     2341 iplprlttpt ivpavndpdv glkvldnglg nvstatttts iststtngaq qlkesneacc
     2401 nraaaasgsg svigrdplpp ravstsqlaq rtqtqppttv stlplsrnna shrqplplqq
     2461 stpqsvrqsd tdamsnsqll raasngdigt nsvminsseg kdqrltspgg hsnqrlispa
     2521 galrvvslnd hrvsspsdsd irihspiern mispigrevd rgggtaarqn qrawecnell
     2581 hkrsnvtgck laqpgthglv rvsagellgr theelvllli qlrrqnatiy kametchmei
     2641 eaqarlaeld tprrlenlqk leelkkhlmd lekqyekgkp lvnlvdnmvk lgslynrnta
     2701 ngtssvsgsr hdlahdnsrd hrlefnqkvq eqrllaeerr dwdrlspdhg qlqakvqqly
     2761 kldrllqees gtlhslqqdk eilekalggl rhklqgsrsn laeaeryrkq qlllerelsr
     2821 vrvllahnsk kleetvaena rleqdlvvlr qklqasrrya gnmtrdtsat tgplevelrr
     2881 vqqlvgdlqr qrkelsvqvr qltekshslv qqirpqppsa pqvhqpkkrt qnswletdld
     2941 sgitldhgld spsspalsss prgkqnggsy lhfssstqik dssptnrqqn hqtfpqspqn
     3001 hisslspqlr eqiqqhqlkq qllkdqmqgk gnlmqanvap lyvntesrmh ennkheenlt
     3061 ngtvipppey ipppppppln eetllndnyr qnednkfagl ihnrekqeik tvrivkrese
     3121 rrqrdrgdrt gnigipltng lqapggakrl cdddlggsqk fekaqlgkvv eespmihaqs
     3181 tvqltdlddv qfqrsmslpr gfggqrqqqp eihqvpvvpp prsdsmhalr smlarrnkvr
     3241 fesqdvssds tlspytdsht ssshlpmssp nystspsysp sqqpypgqpi rsnqqavyyq
     3301 qyrhyenpis sraentgdpv spellsptsp dvqdrafgst lnvnasnspq lspvfkseaa
     3361 kqiikemter kvegprrrqi prekrrhytv ssskpvldle dtfskmgmgr arddldmera
     3421 lrprinapdv vrstlshkel kynestidql lgtpnkivip eryipeqtpe ltaeeqqhrl
     3481 kkaeairkml settvtateg nddninieks dnlkrkvvee krqrdhilql nqilakqvme
     3541 kskmvavkal atlplntess lddedlspvt plplyqqren yys
//
LOCUS       XP_076546645            3583 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546645
VERSION     XP_076546645.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690530.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3583
                     /product="uncharacterized protein LOC117611843 isoform X5"
                     /calculated_mol_wt=401150
     Region          50..152
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(64,66..67,74,76,87,130)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2641..>2940
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3583
                     /gene="LOC117611843"
                     /coded_by="XM_076690530.1:1944..12695"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mdskkvvqaa plpvpvqvrs kndgsgvaas vsgvrrrsgs qglrspaakr plsapvalqg
       61 wlhkqgsegl mlwkkrwfvl seyclfyykg peeekllgsi llpsykvtvc kpedkvnrkf
      121 afkaehanmr tyhfaadsfe smnqwvnalt latllqdpnp glgeaavvie iagqqdgers
      181 arpsvssiss ilnhsaddsd sgfhgfqsrd dpshasnnns spnsvntasf pnlsgsnsns
      241 ttnninnnnt nnnvalvnsn nnvnevqpmv ngwiqqqtpy iqastsqqqq qygqlmpspq
      301 qlqqamlhqh ishqnsqsah qvhqhkhvvq qqqsqshlas nvqtvqpmpr kfgqpiyana
      361 ppkprrltdg steystpspd pdyrkspvsp dvtvtskspm sdydrnsmiy gtrmsqpsqq
      421 slrtdksgln ygytgqvqte rrtpdtygrs aakprsnhrg ngdyeevyga pqlyqrpagp
      481 vgytkgaspa pipiplyaqq hqqyqqqihs pvaasnvpim rqpraqpppr phsadfleye
      541 anrkpqqqlp pqceeslnqq rrpqrpkssl divtpsdpan dgyfyseery aaqmrqsavy
      601 lhqtpqhhqq qrnqslsrat mpskatgird grsedgriat lpeasctglr rgqrehvhqq
      661 hvmphqslqr htemnsneak mrrslreksy epssremlsh vedgtvprri prdyessmgw
      721 grsttshtsq gahvgqpcha ahgaarrwne qqqfcrsasa rlprtrhpaa ldpddddyge
      781 rsseqdsrdg erkiqqrees mkrllewkqr mlqspltrkp sgsanrgrmq nelssyykqq
      841 allelaahea svaenrhsrr redghrphar skstdgrrtv anvsrynsys sddeelgdvr
      901 rkrtrrpsha grsprqlgdn rssgipvqdt vpgsgrqnpn vprcsndhrt spnasvlpnl
      961 ggippdagye evsfppsqrt drspsyflse ahrsvgrcdq qeldasydee kfkkkpsgil
     1021 ktsatygveq snkspnssss sgypaeswsg qktvqwttkk erddwdsnid eskvikefsy
     1081 qyikpkeeve saederspgk peamnlvqcr irsfesnidn sspvkevlam qeplkvsplq
     1141 ateahvskld cskksssiir nfalgeaeaq dskyrnvkeg hakqastdsn ksvkdlladf
     1201 erksqqvqrq eygrrqevkh rgvfsdseal lydtgsdldq rekneetgne rdsktdqivp
     1261 epindykpya datkkkgdas frrkkrspsk esttdeipne dldtvsnpsc nrlsvtesll
     1321 thgdhfsges gttsptvkqe dinpeehylp msprkaildp ncddrpnpnm meslfatleh
     1381 eessyvemaq ngmthsllap teenrkhdsg dystldtphy efvcvsdskm epvymevsql
     1441 sekeekdsgk lskkrshdds nhsrtdlpdi lttlksdssd addesskdld sidaprhprf
     1501 slsdtfrpas yylgasrtmi aelhdssdse lvspppiptt ppplddldsl dmqesleikk
     1561 vspqvavmkd nssnrdspks wnkpmgqves hrppsrfsda tisstfrdsr islesasgsd
     1621 svelrtpeee arhrqlktrp vsevcevlds ldeleslgsr fdgasidldq yleelqarda
     1681 fnvdlyakdl synsiygfne nmlvvdklqk ttchdlsgqv nlgfdnleqs mrygngnkmg
     1741 sasslpsmrv sdvvppshqh sqsftgdahy enitsfsplr gspairsdne qtrdvghrlq
     1801 rgsqnnllta shsrdssysi asaaasists macqrpasaq ssmhsptgsi slgnhhgnnl
     1861 ptsvlrntsp ysdqrfqnhs rsasqdsary smisnhrsss sqdsnhypts tptvktnhps
     1921 qsylpnetra qneqsgapyy ysdlqasana vdsesskphi prlpqlnnqr ddasalnkkn
     1981 digrivnpis rqmprqiqvd didearriaa elrkttcqll gdnkvdlvdk knfyeadtlr
     2041 rvkstdplpd issaeisprn lyphglrdks ssqasnretf elthgtqtsh rrsrslegll
     2101 ddvglqnlve hrnrsnrvat vqptqvdemp qsmgtsnvtg snsnfsaedp weqdslwres
     2161 lrrvslrnar sldnldsppr psvstsdtks rnkvtrgaty vndsvilrre ncmeeqqser
     2221 prvkrrpnkd eerllddlty eslsmdriha ggyvwdaene ayrkatdedr nhfleegnlp
     2281 pvrsisesqv sastsaafel dreklrqwdl lssacllqeq qrgsmadsgr glpiverpgd
     2341 iplprlttpt ivpavndpdv glkvldnglg nvstatttts iststtngaq qlkesneacc
     2401 nraaaasgsg svigrdplpp ravstsqlaq rtqtqppttv stlplsrnna shrqplplqq
     2461 stpqsvrqsd tdamsnsqll raasngdigt nsvminsseg kdqrltspgg hsnqrlispa
     2521 galrvvslnd hrvsspsdsd irihspiern mispigrevd rgggtaarqn qrawecnell
     2581 hkrsnvtgck laqpgthglv rvsagellgr theelvllli qlrrqnatiy kametchmei
     2641 eaqarlaeld tprrlenlqk leelkkhlmd lekqyekgkp lvnlvdnmvk lgslynrnta
     2701 ngtssvsgsr hdlahdnsrd hrlefnqkvq eqrllaeerr dwdrlspdhg qlqakvqqly
     2761 kldrllqees gtlhslqqdk eilekalggl rhklqgsrsn laeaeryrkq qlllerelsr
     2821 vrvllahnsk kleetvaena rleqdlvvlr qklqasrrya gnmtrdtsat tgplevelrr
     2881 vqqlvgdlqr qrkelsvqvr qltekshslv qqirpqppsa pqvhqpkkrt qnswletdld
     2941 sgitldhgld spsspalsss prgkqnggsy lhfssstqik dssptnrqqn hqtfpqspqn
     3001 hisslspqlr eqiqqhqlkq qllkdqmqgk gnlmqanvap lyvntesrmh ennkheenlt
     3061 ngtvipppey ipppppppln eetllndnyr qnednkfagl ihnrekqeik tvrivkrese
     3121 rrqrdrgdrt gnigipltng lqapggakrl cdddlggsqk fekaqlgkvv eespmihaqs
     3181 tvqltdlddv qfqrsmslpr gfggqrqqqp eihqvpvvpp prsdsmhalr smlarrnkvr
     3241 fesqdvssds tlspytdsht ssshlpmssp nystspsysp sqqpypgqpi rsnqqavyyq
     3301 qyrhyenpis sraentgdpv spellsptsp dvqdrafgst lnvnasnspq lspvfkseaa
     3361 kqiikemter kvegprrrqi prekrrhytv ssskpvldle dtfskmgmgr arddldmera
     3421 lrprinapdv vrstlshkel kynestidql lgtpnkivip eryipeqtpe ltaeeqqhrl
     3481 kkaeairkml settvtateg nddninieks dnlkrkvvee krqrdhilql nqilakqvme
     3541 kskmvavkal atlplntess lddedlspvt plplyqqren yys
//
LOCUS       XP_076546646            3552 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611843 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546646
VERSION     XP_076546646.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690531.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3552
                     /product="uncharacterized protein LOC117611843 isoform X6"
                     /calculated_mol_wt=397306
     Region          185..287
                     /region_name="PH_PEPP1_2_3"
                     /note="Phosphoinositol 3-phosphate binding proteins 1, 2,
                     and 3 pleckstrin homology (PH) domain; cd13248"
                     /db_xref="CDD:270068"
     Site            order(199,201..202,209,211,222,265)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:270068"
     Region          2776..>3075
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..3552
                     /gene="LOC117611843"
                     /coded_by="XM_076690531.1:1481..12139"
                     /db_xref="GeneID:117611843"
ORIGIN      
        1 mskccscigg yttdtyqpls shisrgsvkl pkdsrdafrs prnqqrklsv rydvvrsvgs
       61 asdpstphrh lagslrgsvn yshnhrhnhl hrhdpplppa yctppppsss syptvqrgca
      121 lhsrldqpvt sstsgmdskk vvqaaplpvp vqvrskndgs gvaasvsgvr rrsgsqglrs
      181 paakrplsap valqgwlhkq gseglmlwkk rwfvlseycl fyykgpeeek llgsillpsy
      241 kvtvckpedk vnrkfafkae hanmrtyhfa adsfesmnqw vnaltlatll qdpnpglgea
      301 avvieiagqq dgersarpsv ssissilnhs addsdsgfhg fqsrddpsha snnnsspnsv
      361 ntasfpnlsg snsnsttnni nnnntnnnva lvnsnnnvne vqpmvngwiq qqtpyiqast
      421 sqqqqqygql mpspqqlqqa mlhqhishqn sqsahqvhqh khvvqqqqsq shlasnvqtv
      481 qpmprkfgqp iyanappkpr rltdgsteys tpspdpdyrk spvspdvtvt skspmsdydr
      541 nsmiygtrms qpsqqslrtd ksglnygytg qvqterrtpd tygrsaakpr snhrgngdye
      601 evygapqlyq rpagpvgytk gaspapipip lyaqqhqqyq qqihspvaas nvpimrqpra
      661 qppprphsad fleyeanrkp qqqlppqcee slnqqrrpqr pkssldivtp sdpandgyfy
      721 seeryaaqmr qsavylhqtp qhhqqqrnqs lsratmpska tgirdgrsed griatlpeas
      781 ctglrrgqre hvhqqhvmph qslqrhtemn sneakmrrsl reksyepssr emlshvedgt
      841 vprriprdye ssmgwgrstt shtsqgahvg qpchaahgaa rrwneqqqfc rsasarlprt
      901 rhpaaldpdd ddygersseq dsrdgerkiq qreesmkrll ewkqrmlqsp ltrkpsgsan
      961 rgrmqnelss yykqqallel aaheasvaen rhsrrredgh rpharskstd grrtvanvsr
     1021 ynsyssddee lgdvrrkrtr rpshagrspr qlgdnrssgi pvqdtvpgsg rqnpnvprcs
     1081 ndhrtspnas vlpnlggipp dagyeevsfp psqrtdrsps yflseahrsv grcdqqelda
     1141 sydeekfkkk psgilktsat ygveqsnksp nsssssgypa eswsgqktvq wttkkerddw
     1201 dsnideskvi kefsyqyikp keevesaede rspgkpeamn lvqcrirsfe snidnsspvk
     1261 evlamqeplk vsplqateah vskldcskks ssiirnfalg eaeaqdskyr nvkeghakqa
     1321 stdsnksvkd lladferksq qvqrqeygrr qevkhrgvfs dseallydtg sdldqrekne
     1381 etgnerdskt dqivpepind ykpyadatkk kgdasfrrkk rspskesttd eipnedldtv
     1441 snpscnrlsv tesllthgdh fsgesgttsp tvkqedinpe ehylpmsprk aildpncddr
     1501 pnpnmmeslf atleheessy vemaqngmth sllapteenr khdsgdystl dtphyefvcv
     1561 sdskmepvym evsqlsekee kdsgklskkr shddsnhsrt dlpdilttlk sdssdaddes
     1621 skdldsidap rhprfslsdt frpasyylga srtmiaelhd ssdselvspp pipttpppld
     1681 dldsldmqes leikkvspqv avmkdnssnr dspkswnkpm gqveshrpps rfsdatisst
     1741 frdsrisles asgsdsvelr tpeeearhrq lktrpvsevc evldsldele slgsrfdgas
     1801 idldqyleel qardafnvdl yakdlsynsi ygfnenmlvv dklqkttchd lsgqvnlgfd
     1861 nleqsmrygn gnkmgsassl psmrvsdvvp pshqhsqsft gdahyenits fsplrgspai
     1921 rsdneqtrdv ghrlqrgsqn nlltashsrd ssysiasaaa sistsmacqr pasaqssmhs
     1981 ptgsislgnh hgnnlptsvl rntspysdqr fqnhsrsasq dsarysmisn hrssssqdsn
     2041 hyptstptvk tnhpsqsylp netraqneqs gapyyysdlq asanavdses skphiprlpq
     2101 lnnqrddasa lnkkndigri vnpisrqmpr qiqvddidea rriaaelrkt tcqllgdnkv
     2161 dlvdkknfye adtlrrvkst dplpdissae isprnlyphg lrdksssqas nretfelthg
     2221 tqtshrrsrs legllddvgl qnlvehrnrs nrvatvqptq vdempqsmgt snvtgsnsnf
     2281 saedpweqds lwreslrrvs lrnarsldnl dspprpsvst sdtksrnkvt rgatyvndsv
     2341 ilrrencmee qqserprvkr rpnkdeerll ddltyeslsm drihaggyvw daeneayrka
     2401 tdedrnhfle egnlppvrsi sesqvsasts aafeldrekl rqwdllssac llqeqqrgsm
     2461 adsgrglpiv erpgdiplpr lttptivpav ndpdvglkvl dnglgnvsta ttttsistst
     2521 tngaqqlkes neaccnraaa asgsgsvigr dplppravst sqlaqrtqtq ppttvstlpl
     2581 srnnashrqp lplqqstpqs vrqsdtdams nsqllraasn gdigtnsvmi nssegkdqrl
     2641 tspgghsnqr lispagalrv vslndhrvss psdsdirihs piernmispi grevdrgggt
     2701 aarqnqrawe cnellhkrsn vtgcklaqpg thglvrvsag ellgrtheel vllliqlrrq
     2761 natiykamet chmeieaqar laeldtprrl enlqkleelk khlmdlekqy ekgkplvnlv
     2821 dnmvklgsly nrntangtss vsgsrhdlah dnsrdhrlef nqkvqeqrll aeerrdwdrl
     2881 spdhgqlqak vqqlykldrl lqeesgtlhs lqqdkeilek algglrhklq gsrsnlaeae
     2941 ryrkqqllle relsrvrvll ahnskkleet vaenarleqd lvvlrqklqa srryagnmtr
     3001 dtsattgple velrrvqqlv gdlqrqrkel svqvrqltek shslvqqirp qppsapqvhq
     3061 pkkrtqnswl etdldsgitl dhgldspssp alsssprgkq nggsylhfss stqikdsspt
     3121 nrqqnhqtfp qspqnhissl spqlreqiqq hqlkqqllkd qmqgkgnlmq anvaplyvnt
     3181 esrmhennkh eenltngtvi pppeyipppp ppplneetll ndnyrqnedn kfaglihnre
     3241 kqeiktvriv kreserrqrd rgdrtgnigi pltnglqapg gakrlcdddl ggsqkfekaq
     3301 lgkvveespm ihaqstvqlt dlddvqfqrs mslprgfggq rqqqpeihqv pvvppprsds
     3361 mhalrsmlar rnkvrgmgra rddldmeral rprinapdvv rstlshkelk ynestidqll
     3421 gtpnkivipe ryipeqtpel taeeqqhrlk kaeairkmls ettvtategn ddninieksd
     3481 nlkrkvveek rqrdhilqln qilakqvmek skmvavkala tlplntessl ddedlspvtp
     3541 lplyqqreny ys
//
LOCUS       XP_076546647            1083 aa            linear   INV 03-SEP-2025
DEFINITION  integrator complex subunit 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546647
VERSION     XP_076546647.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690532.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1083
                     /product="integrator complex subunit 3 isoform X1"
                     /calculated_mol_wt=123002
     Region          58..464
                     /region_name="Ints3_N"
                     /note="Integrator complex subunit 3 N-terminal; pfam10189"
                     /db_xref="CDD:431123"
     CDS             1..1083
                     /gene="IntS3"
                     /coded_by="XM_076690532.1:253..3504"
                     /db_xref="GeneID:117611859"
ORIGIN      
        1 meqaktpasr llstscienk ddleekferc ytvlqnltag lsekeahdtl nnavckdkth
       61 eevslgllvv iltdpqsaak syrdltlitr dglaivlghl nqlvlerylr lndvtrsqll
      121 wllremirts vasvdnlcls llrhaaggdi sprnlflvda lldifqenrp wldkfpflva
      181 sivytylrli edhnaphlsg lrqkevtftv slirermvdc lvigrdlvrl lqnvaripef
      241 ealwkdmlln pkslcpnfng vlqllqtrts rrflqsrltp dmerklvflt sqvrfgnhkr
      301 yqdwfqrqyl atpesqslrc dlirfivgvi hptnellcsd iiprwavigw lfttctstva
      361 asnaklalfy dwlffepekd nimniepail vmhnsmrshp pvtatlldfl criipnfypp
      421 ltekvrngif sslrqilekr vlpslyplfd spkldrelrs kiretfkefc lppnadpagn
      481 kvpvysgkme elnkdltpga ilenaanapv ennhvnqdpe pafsdeeeei plrivtkvee
      541 edeedvplan vklkneqknt ncvvkkedit sqlnlilepe lrtavdslhs etdnevrcqt
      601 merivqmvve deidaetipg lascistils sqitsqifpt dnlneealtd sistplfvmf
      661 rnqfqlckee dnrrkllarv laemqnvqsr igylllyflk vwgreeekre gepsnvlndv
      721 kasvykdfca hrekkldacl vsdlklched nifmlcylvp diymgfqnvt lgnvqllhlv
      781 vstvdacqlq elvcqimqgh lkmlkkesft slltaslnwe tfeqycfwql ifahdfpidy
      841 vlpvlpklqf rdhaealtsi llmlkqekpt lellrqllar qnvdgdmfvv aalrywcrdy
      901 eeklgellan llstrypats pnkrkrsggk hnqqpgppsg eqvlghldql rqhctssael
      961 qlyhsegmqr alqqaqaass dslrksygdl falaevneen epppppptss arkhaaastg
     1021 agggagkggh rktgantrer ssskrpppry hltstsssee eeivnlkqak krkkinpvgs
     1081 dsd
//
LOCUS       XP_076546648            1074 aa            linear   INV 03-SEP-2025
DEFINITION  integrator complex subunit 3 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546648
VERSION     XP_076546648.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690533.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1074
                     /product="integrator complex subunit 3 isoform X2"
                     /calculated_mol_wt=122086
     Region          58..464
                     /region_name="Ints3_N"
                     /note="Integrator complex subunit 3 N-terminal; pfam10189"
                     /db_xref="CDD:431123"
     CDS             1..1074
                     /gene="IntS3"
                     /coded_by="XM_076690533.1:253..3477"
                     /db_xref="GeneID:117611859"
ORIGIN      
        1 meqaktpasr llstscienk ddleekferc ytvlqnltag lsekeahdtl nnavckdkth
       61 eevslgllvv iltdpqsaak syrdltlitr dglaivlghl nqlvlerylr lndvtrsqll
      121 wllremirts vasvdnlcls llrhaaggdi sprnlflvda lldifqenrp wldkfpflva
      181 sivytylrli edhnaphlsg lrqkevtftv slirermvdc lvigrdlvrl lqnvaripef
      241 ealwkdmlln pkslcpnfng vlqllqtrts rrflqsrltp dmerklvflt sqvrfgnhkr
      301 yqdwfqrqyl atpesqslrc dlirfivgvi hptnellcsd iiprwavigw lfttctstva
      361 asnaklalfy dwlffepekd nimniepail vmhnsmrshp pvtatlldfl criipnfypp
      421 ltekvrngif sslrqilekr vlpslyplfd spkldrelrs kiretfkefc lppnadpgkm
      481 eelnkdltpg ailenaanap vennhvnqdp epafsdeeee iplrivtkve eedeedvpla
      541 nvklkneqkn tncvvkkedi tsqlnlilep elrtavdslh setdnevrcq tmerivqmvv
      601 edeidaetip glascistil ssqitsqifp tdnlneealt dsistplfvm frnqfqlcke
      661 ednrrkllar vlaemqnvqs rigylllyfl kvwgreeekr egepsnvlnd vkasvykdfc
      721 ahrekkldac lvsdlklche dnifmlcylv pdiymgfqnv tlgnvqllhl vvstvdacql
      781 qelvcqimqg hlkmlkkesf tslltaslnw etfeqycfwq lifahdfpid yvlpvlpklq
      841 frdhaealts illmlkqekp tlellrqlla rqnvdgdmfv vaalrywcrd yeeklgella
      901 nllstrypat spnkrkrsgg khnqqpgpps geqvlghldq lrqhctssae lqlyhsegmq
      961 ralqqaqaas sdslrksygd lfalaevnee neppppppts sarkhaaast gagggagkgg
     1021 hrktgantre rssskrpppr yhltstssse eeeivnlkqa kkrkkinpvg sdsd
//
LOCUS       XP_034196121             960 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 39 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196121
VERSION     XP_034196121.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340230.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196121.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..960
                     /product="coiled-coil domain-containing protein 39 isoform
                     X1"
                     /calculated_mol_wt=112644
     Region          128..802
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..960
                     /gene="LOC117611845"
                     /coded_by="XM_034340230.2:57..2939"
                     /db_xref="GeneID:117611845"
ORIGIN      
        1 mtmninidev ltalgwqdgf ripvaneenk qleeeiekki klkvnlttkl qtteerikmm
       61 ekrinnlmte qdmnqniint hsvqlenedh hyrlscstes nlrqearnfe kewksvnetv
      121 snietelekm tkkiessket ikydekglqe leemlsqned nnqlieqyik edlkeykele
      181 lkrqklstel qsyrdtiiki tneaqemnii lsrtstlydq tlieyqqifn qwsesvtmlq
      241 qrnndiknai qeietlheit qgkkkvlees eqflkeqnen nkqieesikk lekdlfrmke
      301 eqqklketfg tyenqliihn nnakkltqhm qqmqadikhk esqiqkkytk leyvnkqvsd
      361 ltkklhsidn qktgieekak evenmiaeqe kkkskitkei nrlqagnlrv inqikdlene
      421 nkilkmqyqk elkkfeylnk lytkdeqvye ekkealymve fevqkaemkl drlrgneydk
      481 teverkqqni eelqnvlnek mkiskllqkq itsledemrk msnnlandnn elaslknkrq
      541 dlvllmnage kelksaqnry eekqveesml rlkvsqmekt isnlgnnvyd lerhqlelea
      601 avrerkteia iqkeslviqk rvassecsel rsaiaernir ikqlqarydn yvamlgtntd
      661 gtpvntthlk iqnaqekyll qeegdkldet irkteqeiqa mentlrivna cndkykatln
      721 veedksetee hkkldnelqn aekdlkqkqe qlqsltedmq kmqedyakvl kdieetqeyk
      781 enknqyltnl kqqiqeqkek isradknlqn akkgiqkifm ntgnktliiq ekevqlrelq
      841 eqnsivlqdi aeftihhiet eayikkllav knielpsvpi lnqspvssqc hssansvvcv
      901 pkstnrgsic gssrgsighv vniepefeta satnsskqsi rlkqqcvkqs lqtqstkrtf
//
LOCUS       XP_076546649             819 aa            linear   INV 03-SEP-2025
DEFINITION  coiled-coil domain-containing protein 39 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546649
VERSION     XP_076546649.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690534.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..819
                     /product="coiled-coil domain-containing protein 39 isoform
                     X2"
                     /calculated_mol_wt=97059
     Region          128..802
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..819
                     /gene="LOC117611845"
                     /coded_by="XM_076690534.1:57..2516"
                     /db_xref="GeneID:117611845"
ORIGIN      
        1 mtmninidev ltalgwqdgf ripvaneenk qleeeiekki klkvnlttkl qtteerikmm
       61 ekrinnlmte qdmnqniint hsvqlenedh hyrlscstes nlrqearnfe kewksvnetv
      121 snietelekm tkkiessket ikydekglqe leemlsqned nnqlieqyik edlkeykele
      181 lkrqklstel qsyrdtiiki tneaqemnii lsrtstlydq tlieyqqifn qwsesvtmlq
      241 qrnndiknai qeietlheit qgkkkvlees eqflkeqnen nkqieesikk lekdlfrmke
      301 eqqklketfg tyenqliihn nnakkltqhm qqmqadikhk esqiqkkytk leyvnkqvsd
      361 ltkklhsidn qktgieekak evenmiaeqe kkkskitkei nrlqagnlrv inqikdlene
      421 nkilkmqyqk elkkfeylnk lytkdeqvye ekkealymve fevqkaemkl drlrgneydk
      481 teverkqqni eelqnvlnek mkiskllqkq itsledemrk msnnlandnn elaslknkrq
      541 dlvllmnage kelksaqnry eekqveesml rlkvsqmekt isnlgnnvyd lerhqlelea
      601 avrerkteia iqkeslviqk rvassecsel rsaiaernir ikqlqarydn yvamlgtntd
      661 gtpvntthlk iqnaqekyll qeegdkldet irkteqeiqa mentlrivna cndkykatln
      721 veedksetee hkkldnelqn aekdlkqkqe qlqsltedmq kmqedyakvl kdieetqeyk
      781 enknqyltnl kqqiqeqkek isradknlqn akkergttt
//
LOCUS       XP_034196125             941 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 2, subunit alpha isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196125
VERSION     XP_034196125.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340234.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..941
                     /product="adaptor protein complex 2, subunit alpha isoform
                     X1"
                     /calculated_mol_wt=105259
     Region          28..591
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          714..817
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     Region          823..936
                     /region_name="Alpha_adaptin_C"
                     /note="Alpha adaptin AP2, C-terminal domain; pfam02296"
                     /db_xref="CDD:426707"
     CDS             1..941
                     /gene="AP-2alpha"
                     /coded_by="XM_034340234.2:164..2989"
                     /db_xref="GeneID:117611846"
ORIGIN      
        1 mpavrgdgmr glavfisdir nckskeaeik rinkelanir skfkgdktld gyqkkkyvck
       61 llfifllghd idfghmeavn llssnkysek qigylfisvl vntnsdlikl iiqsikndla
      121 srnpihvnla lqcianigsk emaeafgnei pkllvsgdtm dvvkqsaalc llrllrtapd
      181 vvpggewtsr ivhllndqhl gvvtaaasli dalvkrnpde ykgcvslavs rlsrivtssy
      241 tdlqdytyyf vpapwlsvkl lrllqnytpp aedpgvrgrl necletilnk aqeppkskkv
      301 qhsnaknavl feaisliihn dselplsvra cnqlgqflsn retnlrylal esmchlatse
      361 lsheavkkhq evvilsmkme kdvsvrqqav dllyamcdks naeeivqeml nyletadysi
      421 reemvlkvai laekyatdyt wyvdvilnli riagdyvsee vwyrviqivi nrddvqgyaa
      481 kmvfealqap achenmvkvg gyilgefgnl iagdqrsspa vqfqllhsky hlcspmtral
      541 llstyikfvn lfpemrsqiq dvfrqhsnlr sadaelqqra seylqlsiia ssdvlatvle
      601 empafperes silavlkkkk pgrvpeneir eskspapntn hhpeipataa ptvnntsadl
      661 lglstppssq ptsntgvlld vlgdiynmpn kvatngaqnt ynpkkfvckn ngvlfendli
      721 qigvksefrq nlgrvglfyg nktqyalhsf qpqlswseen qaklavqvkp vdpvleagaq
      781 iqqminaeci ddysdapsmi isfiynnvpq kitmklplti nkffeptemn gesffarwkn
      841 lggtisranq qrsqkifkaq qpmdlaqvrt klqgfgmqll dgidpnpdnf vcagivhmra
      901 qqvgcllrle pnkqaqmfrl tvrsskesms veicdllvdq f
//
LOCUS       XP_034196126             941 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 2, subunit alpha isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196126
VERSION     XP_034196126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..941
                     /product="adaptor protein complex 2, subunit alpha isoform
                     X1"
                     /calculated_mol_wt=105259
     Region          28..591
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          714..817
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     Region          823..936
                     /region_name="Alpha_adaptin_C"
                     /note="Alpha adaptin AP2, C-terminal domain; pfam02296"
                     /db_xref="CDD:426707"
     CDS             1..941
                     /gene="AP-2alpha"
                     /coded_by="XM_034340235.2:47..2872"
                     /db_xref="GeneID:117611846"
ORIGIN      
        1 mpavrgdgmr glavfisdir nckskeaeik rinkelanir skfkgdktld gyqkkkyvck
       61 llfifllghd idfghmeavn llssnkysek qigylfisvl vntnsdlikl iiqsikndla
      121 srnpihvnla lqcianigsk emaeafgnei pkllvsgdtm dvvkqsaalc llrllrtapd
      181 vvpggewtsr ivhllndqhl gvvtaaasli dalvkrnpde ykgcvslavs rlsrivtssy
      241 tdlqdytyyf vpapwlsvkl lrllqnytpp aedpgvrgrl necletilnk aqeppkskkv
      301 qhsnaknavl feaisliihn dselplsvra cnqlgqflsn retnlrylal esmchlatse
      361 lsheavkkhq evvilsmkme kdvsvrqqav dllyamcdks naeeivqeml nyletadysi
      421 reemvlkvai laekyatdyt wyvdvilnli riagdyvsee vwyrviqivi nrddvqgyaa
      481 kmvfealqap achenmvkvg gyilgefgnl iagdqrsspa vqfqllhsky hlcspmtral
      541 llstyikfvn lfpemrsqiq dvfrqhsnlr sadaelqqra seylqlsiia ssdvlatvle
      601 empafperes silavlkkkk pgrvpeneir eskspapntn hhpeipataa ptvnntsadl
      661 lglstppssq ptsntgvlld vlgdiynmpn kvatngaqnt ynpkkfvckn ngvlfendli
      721 qigvksefrq nlgrvglfyg nktqyalhsf qpqlswseen qaklavqvkp vdpvleagaq
      781 iqqminaeci ddysdapsmi isfiynnvpq kitmklplti nkffeptemn gesffarwkn
      841 lggtisranq qrsqkifkaq qpmdlaqvrt klqgfgmqll dgidpnpdnf vcagivhmra
      901 qqvgcllrle pnkqaqmfrl tvrsskesms veicdllvdq f
//
LOCUS       XP_034196127             937 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 2, subunit alpha isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196127
VERSION     XP_034196127.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340236.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..937
                     /product="adaptor protein complex 2, subunit alpha isoform
                     X2"
                     /calculated_mol_wt=104801
     Region          28..591
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          714..817
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     Region          823..932
                     /region_name="Alpha_adaptin_C"
                     /note="Alpha adaptin AP2, C-terminal domain; pfam02296"
                     /db_xref="CDD:426707"
     CDS             1..937
                     /gene="AP-2alpha"
                     /coded_by="XM_034340236.2:164..2977"
                     /db_xref="GeneID:117611846"
ORIGIN      
        1 mpavrgdgmr glavfisdir nckskeaeik rinkelanir skfkgdktld gyqkkkyvck
       61 llfifllghd idfghmeavn llssnkysek qigylfisvl vntnsdlikl iiqsikndla
      121 srnpihvnla lqcianigsk emaeafgnei pkllvsgdtm dvvkqsaalc llrllrtapd
      181 vvpggewtsr ivhllndqhl gvvtaaasli dalvkrnpde ykgcvslavs rlsrivtssy
      241 tdlqdytyyf vpapwlsvkl lrllqnytpp aedpgvrgrl necletilnk aqeppkskkv
      301 qhsnaknavl feaisliihn dselplsvra cnqlgqflsn retnlrylal esmchlatse
      361 lsheavkkhq evvilsmkme kdvsvrqqav dllyamcdks naeeivqeml nyletadysi
      421 reemvlkvai laekyatdyt wyvdvilnli riagdyvsee vwyrviqivi nrddvqgyaa
      481 kmvfealqap achenmvkvg gyilgefgnl iagdqrsspa vqfqllhsky hlcspmtral
      541 llstyikfvn lfpemrsqiq dvfrqhsnlr sadaelqqra seylqlsiia ssdvlatvle
      601 empafperes silavlkkkk pgrvpeneir eskspapntn hhpeipataa ptvnntsadl
      661 lglstppssq ptsntgvlld vlgdiynmpn kvatngaqnt ynpkkfvckn ngvlfendli
      721 qigvksefrq nlgrvglfyg nktqyalhsf qpqlswseen qaklavqvkp vdpvleagaq
      781 iqqminaeci ddysdapsmi isfiynnvpq kitmklplti nkffeptemn gesffarwkn
      841 lgganqqrsq kifkaqqpmd laqvrtklqg fgmqlldgid pnpdnfvcag ivhmraqqvg
      901 cllrlepnkq aqmfrltvrs skesmsveic dllvdqf
//
LOCUS       XP_034196128             759 aa            linear   INV 03-SEP-2025
DEFINITION  suppressor of Triplolethal [Osmia lignaria lignaria].
ACCESSION   XP_034196128
VERSION     XP_034196128.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340237.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..759
                     /product="suppressor of Triplolethal"
                     /calculated_mol_wt=83131
     Region          10..301
                     /region_name="ELL"
                     /note="RNA polymerase II elongation factor ELL; pfam10390"
                     /db_xref="CDD:463068"
     Region          626..728
                     /region_name="Occludin_ELL"
                     /note="Occludin homology domain; pfam07303"
                     /db_xref="CDD:462140"
     CDS             1..759
                     /gene="Su(Tpl)"
                     /coded_by="XM_034340237.2:719..2998"
                     /db_xref="GeneID:117611847"
ORIGIN      
        1 maalvagvqy glsshsnfhe nkslifvklt dsaqraiedf lrnrnktsqn ptiqflgneg
       61 qlsfpstqss hgsagftfsl sgnqdiegpq ggfeciqqtg pksleslgal pckmriqand
      121 dvyettrhrm avaeennknk ctrvikangp digrkvkvks sgrpipspsn srhrestsip
      181 tsgsqsrlss sykpaasnpp psrnqpekki sdimrrplke rlihllalrp ykkpelydri
      241 nregikdrer nvmttilkqv afmrdntyhl hryvwndvqe dwpyyteqek amlkrrkpqn
      301 ltppgssdgg ssgsgqspns thpgsppait apppsllsnk rpgyyqgndg lptkrprish
      361 ykkpetnsgs snagengrta gggstnsvns avaggaggns asgsgggsgv adgwdqrqhq
      421 rdrrneyrae rtansdvlrg gnygtgkpcl tptsdseetg qighrdaiva stgtsgstas
      481 nnasnshnsh ssvahsssls vgrhhhagks atsgadgngs tsvdyaarsm psvvseggsa
      541 gsysgssngi laerrdrsdr ndrgrgsdrd retrkrnnaa ssnnytdltt pdyagnednt
      601 vttstcsldl aespksseyp dyltyyttis saeqrrryka efnadyeeyr rlhaqvakvs
      661 krfaqlqerl kqeeasgnwd eyeeirrqil hdynetkrdp vhketkrrfh ylheklshik
      721 rlvleydtqn csgnmmtnsn sngnangsne mkgvdslhy
//
LOCUS       XP_034196129             523 aa            linear   INV 03-SEP-2025
DEFINITION  endochitinase [Osmia lignaria lignaria].
ACCESSION   XP_034196129
VERSION     XP_034196129.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340238.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196129.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..523
                     /product="endochitinase"
                     /calculated_mol_wt=59599
     Region          21..387
                     /region_name="GH18_chitinase-like"
                     /note="The GH18 (glycosyl hydrolase, family 18) type II
                     chitinases hydrolyze chitin, an abundant polymer of
                     beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a
                     major component of the cell wall of fungi and the
                     exoskeleton of arthropods. Chitinases have...; cl10447"
                     /db_xref="CDD:471972"
     Site            order(24,55,138,140,142,211..212,266,366)
                     /site_type="active"
                     /db_xref="CDD:119349"
     Region          468..513
                     /region_name="ChtBD2"
                     /note="Chitin-binding domain type 2; smart00494"
                     /db_xref="CDD:214696"
     CDS             1..523
                     /gene="LOC117611848"
                     /coded_by="XM_034340238.2:130..1701"
                     /db_xref="GeneID:117611848"
ORIGIN      
        1 mnllvlpllf viaaaeqqgr ivcyfsnwai yrpdvgrygi ndvpgdlcth iiysfigvsn
       61 vtwevlildp eldvdkgnfl afnnlrtkyp hlktsiavgg wgeggrkysa lvsmkerrdt
      121 fiksvvefmy kydfdgfdld weypaatdrg grfsdkdkff yfveelrraf dkekkgweit
      181 mavpmakfrl degyhvpelc inldaihvma ydlrgnwagf adthsplyrr phdqwayekl
      241 nvhdglllwe ekgcpakklv vgiplygrtf tlsqgnhnye pgtyinkeag ggepgeytqa
      301 kgflsyyeic trlqapgsnw tqkfdnigkv pymyketqwv gyentesiky kidfikgsry
      361 agamvwaidm ddfqglcger nplmntihqg lkgyvvqekn fpttptpdwa kppsttsand
      421 vqttqsssty kptekptqkp evssttedsn ksestqqpvd dneaektvcq stnkfvpskd
      481 chsyfecvhg lpmkftcpnk liwntinnvc dwpqnadree cks
//
LOCUS       XP_034196130             468 aa            linear   INV 03-SEP-2025
DEFINITION  nischarin [Osmia lignaria lignaria].
ACCESSION   XP_034196130
VERSION     XP_034196130.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340239.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..468
                     /product="nischarin"
                     /calculated_mol_wt=53400
     Region          8..123
                     /region_name="PX_IRAS"
                     /note="The phosphoinositide binding Phox Homology domain
                     of the Imidazoline Receptor Antisera-Selected; cd06875"
                     /db_xref="CDD:132785"
     Site            order(43..45,68..69,82)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:132785"
     Region          246..410
                     /region_name="PPP1R42"
                     /note="protein phosphatase 1 regulatory subunit 42;
                     cl42388"
                     /db_xref="CDD:455733"
     Region          275..296
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          297..318
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          319..342
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          343..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          365..389
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..468
                     /gene="LOC117611849"
                     /coded_by="XM_034340239.2:284..1690"
                     /db_xref="GeneID:117611849"
ORIGIN      
        1 maclllnqen vhikipsadt vdgvtyycie vriasikwtv khryndfael hdklvsenyv
       61 kkdilppkkl ignkceafve krrqsleiyl nivynylkka mprelavfld mhiydiffll
      121 qsmaleffte gdsllqksks ykfnpvqlya iserlkqpcp plevvdkkyd fshvldfnsh
      181 lsglviegss dpyrtsniyt salsidlstf kniedltinc ypvdkiyhmg nlrdtvkylk
      241 vnntklrniv elamceevhk nienandshv wlevthldls dnrietidea ikllphiecl
      301 tlnnnllsei snvtllprls qlylasnnft tlpdnlhtkl gyivyidlsq nklttlasfs
      361 klyslegldv scnriekiee vknighlpcl eylrltgnpv stivdyrvkv lepfgkraad
      421 icldnekpnq keldtvsvhq alriakegks ptftasdapl fsaeipsi
//
LOCUS       XP_034196131             405 aa            linear   INV 03-SEP-2025
DEFINITION  glutamine synthetase 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196131
VERSION     XP_034196131.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340240.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="glutamine synthetase 2 isoform X1"
                     /calculated_mol_wt=45133
     Region          54..404
                     /region_name="PLN02284"
                     /note="glutamine synthetase"
                     /db_xref="CDD:177922"
     CDS             1..405
                     /gene="Gs2"
                     /coded_by="XM_034340240.2:145..1362"
                     /db_xref="GeneID:117611850"
ORIGIN      
        1 mspnilvklc gtrvlqnvgi gqkpkwitvn rflsitmsrl mlkespnaal dktllnryle
       61 lpqpentvqa kyiwidgtge nlrsktrtvd fvpkhpselp iwtydgssty qadgansdiy
      121 lcpvaiyddp frrgknklvl cdtyysdmtp tasnkrfkan eameavkdqe pwfgieqeyt
      181 fldfdgrplg wpkngfpgpq gpyycgvgad kvigreivea hyraclyagv kiagtnaevm
      241 psqwefqvgp cvgisagddl wvarfilhrv aeeygvivtl dpkpvdgswn gagahtnfst
      301 kamrnengia eiekaidkls kqhlrhiqay dprggkdner rltgkcetss ihdfsagvan
      361 rnvsiriprg vaedkkgyle drrpssncdp ysvcdalirt cvlne
//
LOCUS       XP_034196132             369 aa            linear   INV 03-SEP-2025
DEFINITION  glutamine synthetase 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196132
VERSION     XP_034196132.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340241.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="glutamine synthetase 2 isoform X2"
                     /calculated_mol_wt=41125
     Region          18..368
                     /region_name="PLN02284"
                     /note="glutamine synthetase"
                     /db_xref="CDD:177922"
     CDS             1..369
                     /gene="Gs2"
                     /coded_by="XM_034340241.2:171..1280"
                     /db_xref="GeneID:117611850"
ORIGIN      
        1 msrlmlkesp naaldktlln rylelpqpen tvqakyiwid gtgenlrskt rtvdfvpkhp
       61 selpiwtydg sstyqadgan sdiylcpvai yddpfrrgkn klvlcdtyys dmtptasnkr
      121 fkaneameav kdqepwfgie qeytfldfdg rplgwpkngf pgpqgpyycg vgadkvigre
      181 iveahyracl yagvkiagtn aevmpsqwef qvgpcvgisa gddlwvarfi lhrvaeeygv
      241 ivtldpkpvd gswngagaht nfstkamrne ngiaeiekai dklskqhlrh iqaydprggk
      301 dnerrltgkc etssihdfsa gvanrnvsir iprgvaedkk gyledrrpss ncdpysvcda
      361 lirtcvlne
//
LOCUS       XP_076546650             333 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid family intramembrane serine protease rho-7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546650
VERSION     XP_076546650.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690535.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="rhomboid family intramembrane serine protease
                     rho-7"
                     /calculated_mol_wt=38655
     Region          131..303
                     /region_name="GlpG"
                     /note="Membrane-associated serine protease, rhomboid
                     family [Posttranslational modification, protein turnover,
                     chaperones]; COG0705"
                     /db_xref="CDD:440469"
     CDS             1..333
                     /gene="rho-7"
                     /coded_by="XM_076690535.1:240..1241"
                     /db_xref="GeneID:117611852"
ORIGIN      
        1 malrpflhlg dnvykcvftf vqlkpkiyva qkyaqirnfs klrdktrlsk nfqkhknghv
       61 vkiwkpltft vmfsgatfig aviweyesir erthrmirny rqwgihrtgw rreievwwns
      121 lsegekiffp icflnvivfl swhvpafrvt mnryfcsnpa ssvlcwplll stfshfsvlh
      181 lmanmyvlyn fstiavkklg keqfvalylt sgvvasfssy lykvmiglqn pslgasgavm
      241 gilgyvctef pntylsiifl pmikftasta iktmmgvdtl gcllrwqrld haahlggvif
      301 gifwqvwgna niwqkrepvl mfwhqlrdqs ksr
//
LOCUS       XP_034196138             314 aa            linear   INV 03-SEP-2025
DEFINITION  surfeit locus protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034196138
VERSION     XP_034196138.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340247.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..314
                     /product="surfeit locus protein 1"
                     /calculated_mol_wt=36876
     Region          78..294
                     /region_name="SURF1"
                     /note="SURF1 superfamily. Surf1/Shy1 has been implicated
                     in the posttranslational steps of the biogenesis of the
                     mitochondrially-encoded Cox1 subunit of cytochrome c
                     oxidase (complex IV). Cytochrome c oxidase (complex IV),
                     the terminal electron-transferring...; cd06662"
                     /db_xref="CDD:119401"
     CDS             1..314
                     /gene="Surf1"
                     /coded_by="XM_034340247.2:359..1303"
                     /db_xref="GeneID:117611853"
ORIGIN      
        1 mkfriskvfs rignqfnvni vqsllnkays irslcnvtni tkpevkpnll lrdkinayns
       61 rknhdpytyf llsiplatfm lgtwqiqrrk wkldliekls srinhqpiel penlqelqsm
      121 eyypvkvkgt flyekeffvg yrsllvdgks adynaffnkq tpqigyhvit plkladrdlt
      181 ilvnrgwips lynnavrnhe nqteneveiv givrlnekrp pfipgnapek svwyyrdlnq
      241 makkadaspv yiemihnkns sefpigsqtk velrnehlsy iitwyslslf tgymwykqfm
      301 krpiafkgkr lsyr
//
LOCUS       XP_034196122             312 aa            linear   INV 03-SEP-2025
DEFINITION  S-formylglutathione hydrolase-like [Osmia lignaria lignaria].
ACCESSION   XP_034196122
VERSION     XP_034196122.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340231.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="S-formylglutathione hydrolase-like"
                     /calculated_mol_wt=35121
     Region          28..312
                     /region_name="alpha/beta hydrolases"
                     /note="A functionally diverse superfamily containing
                     proteases, lipases, peroxidases, esterases, epoxide
                     hydrolases and dehalogenases. The catalytic apparatus
                     typically involves three residues (catalytic triad): a
                     serine, a glutamate or aspartate and a...; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..312
                     /gene="LOC143305203"
                     /coded_by="XM_034340231.2:63..1001"
                     /db_xref="GeneID:143305203"
ORIGIN      
        1 milriihvak lnfcykrlna cnftrykmpd itevssnkcf ggwqkvyshe syelrckmnf
       61 giflppqvee gpvpviywls gltcteanfi qkagaqkyas ehgvilvapd tsprnlnipg
      121 edddwdfgtg agfyvdatke pwnknyrmys yvtkellali nekfpvlphk qsimghsmgg
      181 hgalicalkn pglyktvsaf apisnpiscp wgkkafsgyf ggtetnvawk dwdatelakk
      241 yngppldilv dqgkedkfwk dgqllpenll avakdaglsl vlrfqegydh syffiatfie
      301 dhikhhvkyl ks
//
LOCUS       XP_076546651             243 aa            linear   INV 03-SEP-2025
DEFINITION  sperm-associated antigen 1-like [Osmia lignaria lignaria].
ACCESSION   XP_076546651
VERSION     XP_076546651.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690536.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="sperm-associated antigen 1-like"
                     /calculated_mol_wt=27963
     Region          3..>137
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          9..37
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(10,13..14,17..18,20,44,47..48,51..52,54..55,78,
                     81..82,85..86,89)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          42..72
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          77..105
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..243
                     /gene="LOC143305740"
                     /coded_by="XM_076690536.1:5345..6076"
                     /db_xref="GeneID:143305740"
ORIGIN      
        1 msnpreaevq slkergntcv keqkyeeamf hythaikldp qnyslysnrs faflkmhqyh
       61 famedalmti qlkpdwtkgy frkaevesqt fhfsealqsy ckalslqpne ptileamhrv
      121 aklliqdkra dqqipwlgag vgiilgvivv iadyvftnkp tlthpllmvl ltmsiamlgf
      181 giakgfryfv krqrksllep pvdlfgndke elddieaemn nekekhpkys kaqarqrfkk
      241 gks
//
LOCUS       XP_034196139             226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611854 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196139
VERSION     XP_034196139.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340248.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..226
                     /product="uncharacterized protein LOC117611854 isoform X1"
                     /calculated_mol_wt=25364
     Region          130..211
                     /region_name="metazoan_ACD"
                     /note="Alpha-crystallin domain (ACD) of metazoan
                     alpha-crystallin-type small(s) heat shock proteins (Hsps).
                     sHsps are small stress induced proteins with monomeric
                     masses between 12 -43 kDa, whose common feature is the
                     Alpha-crystallin domain (ACD). sHsps are...; cd06526"
                     /db_xref="CDD:107247"
     Site            order(130..134,141,143,145..146,182,203..204)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:107247"
     CDS             1..226
                     /gene="LOC117611854"
                     /coded_by="XM_034340248.2:160..840"
                     /db_xref="GeneID:117611854"
ORIGIN      
        1 madsgikrni piklgdfsvi dtefsnirer fdaemkkmed emsrfrselm nresnnffks
       61 ttsrhhtsss ehrtsttskt egwdkvdpaa ppkrsafdsf kstttstqns slspqhdsaw
      121 ldglnspliq degdskmlkl rfdvsqytpe eivvktvdnk llvhakheek tesksvyrey
      181 nrefllpkgt npesikssls kdgvltveap lpaigtgekl ipiahq
//
LOCUS       XP_034196140             187 aa            linear   INV 03-SEP-2025
DEFINITION  heat shock protein beta-1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196140
VERSION     XP_034196140.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340249.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="heat shock protein beta-1 isoform X2"
                     /calculated_mol_wt=21025
     Region          91..172
                     /region_name="metazoan_ACD"
                     /note="Alpha-crystallin domain (ACD) of metazoan
                     alpha-crystallin-type small(s) heat shock proteins (Hsps).
                     sHsps are small stress induced proteins with monomeric
                     masses between 12 -43 kDa, whose common feature is the
                     Alpha-crystallin domain (ACD). sHsps are...; cd06526"
                     /db_xref="CDD:107247"
     Site            order(91..95,102,104,106..107,143,164..165)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:107247"
     CDS             1..187
                     /gene="LOC117611854"
                     /coded_by="XM_034340249.2:155..718"
                     /db_xref="GeneID:117611854"
ORIGIN      
        1 madsgikrni piklgdfsvi dtefsnirer fdaemkkmed emsrfrselm nresnnffks
       61 ttstttstqn sslspqhdsa wldglnspli qdegdskmlk lrfdvsqytp eeivvktvdn
      121 kllvhakhee ktesksvyre ynrefllpkg tnpesikssl skdgvltvea plpaigtgek
      181 lipiahq
//
LOCUS       XP_076546652             182 aa            linear   INV 03-SEP-2025
DEFINITION  alpha-crystallin B chain isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546652
VERSION     XP_076546652.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690537.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..182
                     /product="alpha-crystallin B chain isoform X3"
                     /calculated_mol_wt=20203
     Region          86..167
                     /region_name="metazoan_ACD"
                     /note="Alpha-crystallin domain (ACD) of metazoan
                     alpha-crystallin-type small(s) heat shock proteins (Hsps).
                     sHsps are small stress induced proteins with monomeric
                     masses between 12 -43 kDa, whose common feature is the
                     Alpha-crystallin domain (ACD). sHsps are...; cd06526"
                     /db_xref="CDD:107247"
     Site            order(86..90,97,99,101..102,138,159..160)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:107247"
     CDS             1..182
                     /gene="LOC117611854"
                     /coded_by="XM_076690537.1:98..646"
                     /db_xref="GeneID:117611854"
ORIGIN      
        1 mivdrdvfpa ehktviklrr hhtsssehrt sttsktegwd kvdpaappkr safdsfkstt
       61 tstqnsslsp qhdsawldgl nspliqdegd skmlklrfdv sqytpeeivv ktvdnkllvh
      121 akheektesk svyreynref llpkgtnpes iksslskdgv ltveaplpai gtgeklipia
      181 hq
//
LOCUS       XP_076546653             176 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611855 [Osmia lignaria lignaria].
ACCESSION   XP_076546653
VERSION     XP_076546653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690538.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..176
                     /product="uncharacterized protein LOC117611855"
                     /calculated_mol_wt=19203
     Region          <38..101
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     CDS             1..176
                     /gene="LOC117611855"
                     /coded_by="XM_076690538.1:1811..2341"
                     /db_xref="GeneID:117611855"
ORIGIN      
        1 mfpgrglivi algvcciial lqviearpri rhqkggthcs rcgpgwgvvn rctrgrdtec
       61 gkcaqgtysp hhstqpcwic srcgpglyea hpctsttdtv cdschrpapd npdyqrkckd
      121 rtsvflaped aqetgeesvl vndpdgqdql ndgeevlend aeaeamskel rslerf
//
LOCUS       XP_076546654             176 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611855 [Osmia lignaria lignaria].
ACCESSION   XP_076546654
VERSION     XP_076546654.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690539.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..176
                     /product="uncharacterized protein LOC117611855"
                     /calculated_mol_wt=19203
     Region          <38..101
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     CDS             1..176
                     /gene="LOC117611855"
                     /coded_by="XM_076690539.1:1945..2475"
                     /db_xref="GeneID:117611855"
ORIGIN      
        1 mfpgrglivi algvcciial lqviearpri rhqkggthcs rcgpgwgvvn rctrgrdtec
       61 gkcaqgtysp hhstqpcwic srcgpglyea hpctsttdtv cdschrpapd npdyqrkckd
      121 rtsvflaped aqetgeesvl vndpdgqdql ndgeevlend aeaeamskel rslerf
//
LOCUS       XP_076546655             176 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611855 [Osmia lignaria lignaria].
ACCESSION   XP_076546655
VERSION     XP_076546655.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690540.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..176
                     /product="uncharacterized protein LOC117611855"
                     /calculated_mol_wt=19203
     Region          <38..101
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     CDS             1..176
                     /gene="LOC117611855"
                     /coded_by="XM_076690540.1:1753..2283"
                     /db_xref="GeneID:117611855"
ORIGIN      
        1 mfpgrglivi algvcciial lqviearpri rhqkggthcs rcgpgwgvvn rctrgrdtec
       61 gkcaqgtysp hhstqpcwic srcgpglyea hpctsttdtv cdschrpapd npdyqrkckd
      121 rtsvflaped aqetgeesvl vndpdgqdql ndgeevlend aeaeamskel rslerf
//
LOCUS       XP_034196145             176 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611855 [Osmia lignaria lignaria].
ACCESSION   XP_034196145
VERSION     XP_034196145.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340254.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..176
                     /product="uncharacterized protein LOC117611855"
                     /calculated_mol_wt=19203
     Region          <38..101
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     CDS             1..176
                     /gene="LOC117611855"
                     /coded_by="XM_034340254.2:937..1467"
                     /db_xref="GeneID:117611855"
ORIGIN      
        1 mfpgrglivi algvcciial lqviearpri rhqkggthcs rcgpgwgvvn rctrgrdtec
       61 gkcaqgtysp hhstqpcwic srcgpglyea hpctsttdtv cdschrpapd npdyqrkckd
      121 rtsvflaped aqetgeesvl vndpdgqdql ndgeevlend aeaeamskel rslerf
//
LOCUS       XP_034196146             169 aa            linear   INV 03-SEP-2025
DEFINITION  peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196146
VERSION     XP_034196146.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340255.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..169
                     /product="peptidyl-prolyl cis-trans isomerase
                     NIMA-interacting 1"
                     /calculated_mol_wt=18718
     Region          6..36
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          58..169
                     /region_name="PTZ00356"
                     /note="peptidyl-prolyl cis-trans isomerase (PPIase);
                     Provisional"
                     /db_xref="CDD:185573"
     CDS             1..169
                     /gene="LOC117611857"
                     /coded_by="XM_034340255.2:87..596"
                     /db_xref="GeneID:117611857"
ORIGIN      
        1 madeelpsgw ekrlsrstgq hyylniytke sqwdrpdkpa gnnpsgnnps gnnkrdspee
       61 vqcshllvkh sgsrrpsswr eekitrskee aleliksyre qivsgkatfa elaskysdcs
      121 sakrggdlgp fnrgamqkpf eqaafalkvg elsspvhtds gihiiqrta
//
LOCUS       XP_034196148             167 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611858 [Osmia lignaria lignaria].
ACCESSION   XP_034196148
VERSION     XP_034196148.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340257.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196148.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..167
                     /product="uncharacterized protein LOC117611858"
                     /calculated_mol_wt=20244
     Region          24..164
                     /region_name="rRNA_processing"
                     /note="rRNA processing; pfam08524"
                     /db_xref="CDD:400708"
     CDS             1..167
                     /gene="LOC117611858"
                     /coded_by="XM_034340257.2:79..582"
                     /db_xref="GeneID:117611858"
ORIGIN      
        1 mknqiknlsn gkqsekvdnv kkpfnkkkyr iqkysneyki nqweerrkka ilrnlykeld
       61 kdqqqnlkkp lplksndqee naeekklkqk dafkkakqef lrkrdekrkq veealrvkse
      121 reealkkyke kkmqaykkls khtkrgqpvm kdrlelllek iqqqvsk
//
LOCUS       XP_034195535             426 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611628 [Osmia lignaria lignaria].
ACCESSION   XP_034195535
VERSION     XP_034195535.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339644.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195535.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="uncharacterized protein LOC117611628"
                     /calculated_mol_wt=45773
     Region          <6..>215
                     /region_name="COG5025"
                     /note="Transcription factor of the Forkhead/HNF3 family
                     [Transcription]"
                     /db_xref="CDD:227358"
     Region          109..187
                     /region_name="FH_FOXG"
                     /note="Forkhead (FH) domain found in the Forkhead box
                     protein G (FOXG) subfamily; cd20021"
                     /db_xref="CDD:410795"
     Site            order(114,132..133,136,155..156,158..159,162,169,179..181,
                     183)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410795"
     CDS             1..426
                     /gene="LOC117611628"
                     /coded_by="XM_034339644.2:229..1509"
                     /db_xref="GeneID:117611628"
ORIGIN      
        1 mvnmegsseq htvtaspppr aalksnfsir silpeacagt papsvnrsts peishvedse
       61 dssdldvtgd ggtetppldc srnaansvss seqkdskdrq sdekkkcekp pysynalimm
      121 airqspekrl tlngiyeyim rhfpyyennk qgwqnsirhn lslnkcfvkv prhyddpgkg
      181 nywmldpsse dvfiggttgk lrrrttaasr srlaafkrsv vlgglypsay appgwpasly
      241 tlpylhraaa yppasgayst pagypasllp gaatsstsnl pckpqplpat aappahgpfs
      301 merllqppta tgypngitts gipisgnpyd fystlrslaa hqqhqttaam fghnqqpary
      361 hvnqppvlgq ptsataspgs spepmsphsp pvticnstgv qqprsllhsp pqlllkpitv
      421 ltgrqs
//
LOCUS       XP_034194656             170 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610916 [Osmia lignaria lignaria].
ACCESSION   XP_034194656
VERSION     XP_034194656.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338765.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194656.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..170
                     /product="uncharacterized protein LOC117610916"
                     /calculated_mol_wt=18706
     CDS             1..170
                     /gene="LOC117610916"
                     /coded_by="XM_034338765.2:321..833"
                     /db_xref="GeneID:117610916"
ORIGIN      
        1 mnnsntlimy cqvtfldcde ngvynetrti etfstedssl eytsngdrsi qyvqvdstat
       61 ndidddipst mpdnsiciqn qavptystve gndvyiicsc reneacvhsg nknneepieg
      121 disstpinkp sdistkalee lhirtgqrtc ccesgvsstr lpcghiyfcq
//
LOCUS       XP_076546656             264 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305745 [Osmia lignaria lignaria].
ACCESSION   XP_076546656
VERSION     XP_076546656.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690541.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..264
                     /product="uncharacterized protein LOC143305745"
                     /calculated_mol_wt=30776
     Region          43..229
                     /region_name="PDDEXK_lambda_exonuclease-like"
                     /note="Uncharacterized nucleases similar to lambda phage
                     exonuclease; cd22343"
                     /db_xref="CDD:411747"
     Site            order(99,133,142,144)
                     /site_type="active"
                     /db_xref="CDD:411747"
     CDS             1..264
                     /gene="LOC143305745"
                     /coded_by="XM_076690541.1:266..1060"
                     /db_xref="GeneID:143305745"
ORIGIN      
        1 mtritapvqq nqiwkrlyer vhmailhewq tmrhtieaet rdqaknekwi ayrrklltas
       61 nfgkicrlrn dtprantlkn imypqipnlr sleygrenea nalrqlenel gihilpcglf
      121 idavvpylga tpdgivddii vevkcpesak dlspaeaieq lpairrifrg demnkihhyy
      181 yqvqgqlhit nrqcalfclw tpkgmkivkv prndtfwate meeklkkfyk dcmlpeiies
      241 rfnrhqpire ppyiktggtc tstq
//
LOCUS       XP_034195921            3964 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal heavy chain 7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195921
VERSION     XP_034195921.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340030.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195921.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3964
                     /product="dynein axonemal heavy chain 7 isoform X1"
                     /calculated_mol_wt=456383
     Region          672..1073
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          848..3603
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1205..1531
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          2251..2513
                     /region_name="AAA_8"
                     /note="P-loop containing dynein motor region D4;
                     pfam12780"
                     /db_xref="CDD:463701"
     Region          2899..3120
                     /region_name="AAA_9"
                     /note="ATP-binding dynein motor region; pfam12781"
                     /db_xref="CDD:463702"
     Region          3658..3960
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..3964
                     /gene="LOC117611772"
                     /coded_by="XM_034340030.2:2255..14149"
                     /db_xref="GeneID:117611772"
ORIGIN      
        1 mkcskkksvs ktykylllsq kreefrmhll nvikekkrke tmrakrkkcv qrmeeedddd
       61 ddeeeikdfv tkndrdilry yyyltqgidd dyvgsmgtdl lenilsvipi kwrqkfkecl
      121 ndlvkevkee yvlsikksvi efvignslys llnqlvalek sktigemqlp tdhavnyqwn
      181 itkleristl yrpvmrrifd ccyrefsdlk lldeselaay gesyglenfe rtvlrmchrv
      241 kenlsknwlp kiqtilsvaf ernelpsmsn svkcrrffnc ialimesqlr rlyiqtlddy
      301 idylcnkngt chgfdikvtv rhaivlfdpp fntfvetlst imnsmqeava glprvetlls
      361 niscigsdek llqvkvsdkr lercadeiek ligeekigat eqlqefekye nliygaenek
      421 itdfinstdt vehferyqnl iehyyrlygr ikvefprtsf hglfvvhrge lighilatar
      481 kikedlvtkm iqdyqrkart vgnayqhivd raltvpanss elikleefin vtktettgel
      541 ekelreiiky ivfltdysil ttielktnnf afqwyhklpe vfksheeiva skteefqrnl
      601 kkrieqflrd letyakqcne lqywgnidei yrykkkadrl enkliaamdt idkfneeevl
      661 fgwemsqypl rkatadklsp ykklydaacd fltnydtwmn smigsqdped iematgtayr
      721 tvfrlersiq epiakklaet irinieefke hmpvistlgn psmksrhweq vseivgfpik
      781 vdlsttlaki ldygladyvp kfeaiaeaat kegslekall rmhvdwedia ftvnpyrdtg
      841 tyviasvdei qlllddhltk aqtmknslyi rpfeketlew eskllllqdi mdywlqvqat
      901 wmyfepifss adiqqqmpee grrfsavdki wreimslvss dprvmsvigi dkmlerlkkc
      961 tnlldlvqkg ltayldkkrl yfprffflsn delleilset kdptrvqphl kkcfegierl
     1021 sftddmeitt mqssegekvi ledivdtvaa rgqverwlld lersmkksvr aqviasknaf
     1081 lkqvrsmwal ewpgqtilcv sklywtadvt kalssdplqn vknyihtcth qlneivklvr
     1141 gklstqnrtt lealvtldvh grdvvsglvg qrvtdatdfn wlaqlryywm nndlwttmin
     1201 tslkyayeyl gntsrlvitp ltdrcyrtlf salslhlgga pegpagtgkt ettkdlakav
     1261 akqcvvfncs dgldyialgk ffkglastga wscfdefnri dlevlsvvaq qiltiqrain
     1321 agldqllfeg teisldptca vfitmnpgya grtelpdnlk alfrpvammv pdyaliaent
     1381 lysygfyear plsvkivtay rlcseqlssq nhydygmrav ksvliaagnl kikypdeaed
     1441 ililrsikdv nlpkflkedi plfngitsdl fpgvklpepd ytdlntcvrd acselnlqct
     1501 svflqkiqqi yemmivrhgf mivgypfagk saaynvlada lklceqrnli nehavekfvi
     1561 npkavtlghl ygefdpvshe wsdgvlaisy rafatstnen rkwlifdgpi davwiesmnt
     1621 vlddnkklcl msgeiiqlsp vtslifetmd leqaspatvs rcgmiymeph tlgwdpllks
     1681 wlakvpavid ewlknfiyqs lfrrfcdpll ywlfnnnvqk icpmpesnli rsltylmdcf
     1741 vneyhdekit ksmteldlra qiegsfffsc iwaiggiltv ksresfsmlf rgfleknfpt
     1801 dlmetfelpg pieaplkpyi fvmprdslvf dyrftkegkg kwklwsdell dsppiprdip
     1861 vnqiivptve tirytslfrl lighqkpvlf vgptgtgksv ytvdfllrkn dpavnkplii
     1921 nfsaqttakq tqdtimgkld rrrkgvfgap igkrwivfvd dlsmpmkety gaqppiellr
     1981 qwldygewfd tkdktvikli dvnlmcamgp ptsggkdvtp rfkrhftvlt isefddevmt
     2041 mifskiilwh ldtrgfgkef dpcieeivla tldvykesls nllptpakch yvfnlrdfsr
     2101 viqgvllsvp etmpaltnmr rlwvheilrv fgdrliddnd ntwlvgqiri tldkrmnvim
     2161 eklfndlvps kekdskvssy flyfhspssc tvlqkqpali teqelrnlvf cdfadarvdn
     2221 rlyqevpdle rlreivenyl teynamsrkp mnlvlfrfai ehlsricrii kqprshallv
     2281 gvggsgrqsl trlashisdy dvfqievsqq ygvnawhedv kdilkrvtlt elhstflfsd
     2341 tqikeesfle disnmlnsge ipniftveev seicermrii drqrdrsvqt dgspvalfnl
     2401 fvqtvrdqlh ivivvspign nfrnrirkfp alvncctidw lqswpddall avatrflsti
     2461 eltdqeriag idmcqffhis tqnlsteffl rlnrhvyvtp tsylemintf kdllekkrrn
     2521 imdaktryer glgqldetqv qvvamsetlq slrpqlivat qdvqkmlldv nkekeevaef
     2581 ertvkedena aeiyaseaaa ikaecdadla ealpilkraq aaldtlttad vavvramkqp
     2641 pqgvrliaes vcvlkqvkpe riqqpdgsfl edywkaalrm lsdarfldsl lnfdkdnipd
     2701 nviekirkvy lsnpdfdpek ikkvstaceg lcrwvyamse ydkvskvvap kkralaqaqe
     2761 vydtamgtlr akreqlqrvq eklrtleell eqrriafqtm sdkvtdcetk lkraedligg
     2821 lggeytrwsr tavvlgeryh rltgdvvias gvvaylgpft mpfrlqqmsm wiqrctdlkv
     2881 icstdfqlrd ilgeavlirs wnifglpnda ysvdngiiit narrwpliid pqgqanrwik
     2941 nmerknnlni irltqsdysr vlenalqfgq pvllehvgee idavlepvlm ketfkqggal
     3001 cikfgdtive ysdhfrlyit tklrnphylp eiavkvtlln fmitptgled qllgivvake
     3061 rpdlesekna livqgaankq llqetedkil eilslaegnl lddeeavevl tsskdlsndi
     3121 qvkqaaaeqt eksiddarlq ytsiavystv lfftiamlin idpmyqysla wfinlfelai
     3181 dntepadtve krltdltkyf tyslytnvcr slfekdkllf sfllainllk qqgklslpqw
     3241 iflltggigi dnpyanpttw lpskqwnelc nldsvddfsg iretftqhvk ewrhlfdske
     3301 pqngaipppf hklnlfqrml ilrcirpdki ipgvqnfvee qlglqfveap pfdlpssyad
     3361 sncstplifi ltpgtdpgql llafadeqgy svsrlfylsl gqgqgpvaee tiqkavmvgn
     3421 wvvlqnchla iswmgtleni ceglipdtih sefrlwltsy psehfpssvl ensikmtnep
     3481 pkglranilr sytsdpisdp dffescsqpe yfkkllyslc ffhaivqerr kfgpigwnis
     3541 yefnetdlri svlqlhlfld efedvrfdai ryltgecnyg grvtdewdrr tlntilskfy
     3601 caelldekiy yfdetstsyy apnlrefeaf meytrdlpli tqpsvfgmne nadiikdqhe
     3661 tnllfsslil tqesvesgtg lisndeivfk masdilqklp adydillalk kypmsygqsm
     3721 ntvlvqemgr fnkltghvrt slqnikraik gfivmsfele diyeailtnk ipalwlrysy
     3781 pslkplgsyi hdllarlnfl qkwydegppv iywisgfyft qafltgtrqn ysrkyqipid
     3841 llifdffvlh vheiphsppe ngvyihglfl dgarfnnehm tldesfskvl yesmprmwlv
     3901 pvkkedikkr ptylcpvykt serkgvlstt ghstnfviam tlptakppeh wimrgvallc
     3961 qlse
//
LOCUS       XP_076546657            3964 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal heavy chain 7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546657
VERSION     XP_076546657.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690542.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3964
                     /product="dynein axonemal heavy chain 7 isoform X1"
                     /calculated_mol_wt=456383
     Region          672..1073
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          848..3603
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1205..1531
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          2251..2513
                     /region_name="AAA_8"
                     /note="P-loop containing dynein motor region D4;
                     pfam12780"
                     /db_xref="CDD:463701"
     Region          2899..3120
                     /region_name="AAA_9"
                     /note="ATP-binding dynein motor region; pfam12781"
                     /db_xref="CDD:463702"
     Region          3658..3960
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..3964
                     /gene="LOC117611772"
                     /coded_by="XM_076690542.1:2185..14079"
                     /db_xref="GeneID:117611772"
ORIGIN      
        1 mkcskkksvs ktykylllsq kreefrmhll nvikekkrke tmrakrkkcv qrmeeedddd
       61 ddeeeikdfv tkndrdilry yyyltqgidd dyvgsmgtdl lenilsvipi kwrqkfkecl
      121 ndlvkevkee yvlsikksvi efvignslys llnqlvalek sktigemqlp tdhavnyqwn
      181 itkleristl yrpvmrrifd ccyrefsdlk lldeselaay gesyglenfe rtvlrmchrv
      241 kenlsknwlp kiqtilsvaf ernelpsmsn svkcrrffnc ialimesqlr rlyiqtlddy
      301 idylcnkngt chgfdikvtv rhaivlfdpp fntfvetlst imnsmqeava glprvetlls
      361 niscigsdek llqvkvsdkr lercadeiek ligeekigat eqlqefekye nliygaenek
      421 itdfinstdt vehferyqnl iehyyrlygr ikvefprtsf hglfvvhrge lighilatar
      481 kikedlvtkm iqdyqrkart vgnayqhivd raltvpanss elikleefin vtktettgel
      541 ekelreiiky ivfltdysil ttielktnnf afqwyhklpe vfksheeiva skteefqrnl
      601 kkrieqflrd letyakqcne lqywgnidei yrykkkadrl enkliaamdt idkfneeevl
      661 fgwemsqypl rkatadklsp ykklydaacd fltnydtwmn smigsqdped iematgtayr
      721 tvfrlersiq epiakklaet irinieefke hmpvistlgn psmksrhweq vseivgfpik
      781 vdlsttlaki ldygladyvp kfeaiaeaat kegslekall rmhvdwedia ftvnpyrdtg
      841 tyviasvdei qlllddhltk aqtmknslyi rpfeketlew eskllllqdi mdywlqvqat
      901 wmyfepifss adiqqqmpee grrfsavdki wreimslvss dprvmsvigi dkmlerlkkc
      961 tnlldlvqkg ltayldkkrl yfprffflsn delleilset kdptrvqphl kkcfegierl
     1021 sftddmeitt mqssegekvi ledivdtvaa rgqverwlld lersmkksvr aqviasknaf
     1081 lkqvrsmwal ewpgqtilcv sklywtadvt kalssdplqn vknyihtcth qlneivklvr
     1141 gklstqnrtt lealvtldvh grdvvsglvg qrvtdatdfn wlaqlryywm nndlwttmin
     1201 tslkyayeyl gntsrlvitp ltdrcyrtlf salslhlgga pegpagtgkt ettkdlakav
     1261 akqcvvfncs dgldyialgk ffkglastga wscfdefnri dlevlsvvaq qiltiqrain
     1321 agldqllfeg teisldptca vfitmnpgya grtelpdnlk alfrpvammv pdyaliaent
     1381 lysygfyear plsvkivtay rlcseqlssq nhydygmrav ksvliaagnl kikypdeaed
     1441 ililrsikdv nlpkflkedi plfngitsdl fpgvklpepd ytdlntcvrd acselnlqct
     1501 svflqkiqqi yemmivrhgf mivgypfagk saaynvlada lklceqrnli nehavekfvi
     1561 npkavtlghl ygefdpvshe wsdgvlaisy rafatstnen rkwlifdgpi davwiesmnt
     1621 vlddnkklcl msgeiiqlsp vtslifetmd leqaspatvs rcgmiymeph tlgwdpllks
     1681 wlakvpavid ewlknfiyqs lfrrfcdpll ywlfnnnvqk icpmpesnli rsltylmdcf
     1741 vneyhdekit ksmteldlra qiegsfffsc iwaiggiltv ksresfsmlf rgfleknfpt
     1801 dlmetfelpg pieaplkpyi fvmprdslvf dyrftkegkg kwklwsdell dsppiprdip
     1861 vnqiivptve tirytslfrl lighqkpvlf vgptgtgksv ytvdfllrkn dpavnkplii
     1921 nfsaqttakq tqdtimgkld rrrkgvfgap igkrwivfvd dlsmpmkety gaqppiellr
     1981 qwldygewfd tkdktvikli dvnlmcamgp ptsggkdvtp rfkrhftvlt isefddevmt
     2041 mifskiilwh ldtrgfgkef dpcieeivla tldvykesls nllptpakch yvfnlrdfsr
     2101 viqgvllsvp etmpaltnmr rlwvheilrv fgdrliddnd ntwlvgqiri tldkrmnvim
     2161 eklfndlvps kekdskvssy flyfhspssc tvlqkqpali teqelrnlvf cdfadarvdn
     2221 rlyqevpdle rlreivenyl teynamsrkp mnlvlfrfai ehlsricrii kqprshallv
     2281 gvggsgrqsl trlashisdy dvfqievsqq ygvnawhedv kdilkrvtlt elhstflfsd
     2341 tqikeesfle disnmlnsge ipniftveev seicermrii drqrdrsvqt dgspvalfnl
     2401 fvqtvrdqlh ivivvspign nfrnrirkfp alvncctidw lqswpddall avatrflsti
     2461 eltdqeriag idmcqffhis tqnlsteffl rlnrhvyvtp tsylemintf kdllekkrrn
     2521 imdaktryer glgqldetqv qvvamsetlq slrpqlivat qdvqkmlldv nkekeevaef
     2581 ertvkedena aeiyaseaaa ikaecdadla ealpilkraq aaldtlttad vavvramkqp
     2641 pqgvrliaes vcvlkqvkpe riqqpdgsfl edywkaalrm lsdarfldsl lnfdkdnipd
     2701 nviekirkvy lsnpdfdpek ikkvstaceg lcrwvyamse ydkvskvvap kkralaqaqe
     2761 vydtamgtlr akreqlqrvq eklrtleell eqrriafqtm sdkvtdcetk lkraedligg
     2821 lggeytrwsr tavvlgeryh rltgdvvias gvvaylgpft mpfrlqqmsm wiqrctdlkv
     2881 icstdfqlrd ilgeavlirs wnifglpnda ysvdngiiit narrwpliid pqgqanrwik
     2941 nmerknnlni irltqsdysr vlenalqfgq pvllehvgee idavlepvlm ketfkqggal
     3001 cikfgdtive ysdhfrlyit tklrnphylp eiavkvtlln fmitptgled qllgivvake
     3061 rpdlesekna livqgaankq llqetedkil eilslaegnl lddeeavevl tsskdlsndi
     3121 qvkqaaaeqt eksiddarlq ytsiavystv lfftiamlin idpmyqysla wfinlfelai
     3181 dntepadtve krltdltkyf tyslytnvcr slfekdkllf sfllainllk qqgklslpqw
     3241 iflltggigi dnpyanpttw lpskqwnelc nldsvddfsg iretftqhvk ewrhlfdske
     3301 pqngaipppf hklnlfqrml ilrcirpdki ipgvqnfvee qlglqfveap pfdlpssyad
     3361 sncstplifi ltpgtdpgql llafadeqgy svsrlfylsl gqgqgpvaee tiqkavmvgn
     3421 wvvlqnchla iswmgtleni ceglipdtih sefrlwltsy psehfpssvl ensikmtnep
     3481 pkglranilr sytsdpisdp dffescsqpe yfkkllyslc ffhaivqerr kfgpigwnis
     3541 yefnetdlri svlqlhlfld efedvrfdai ryltgecnyg grvtdewdrr tlntilskfy
     3601 caelldekiy yfdetstsyy apnlrefeaf meytrdlpli tqpsvfgmne nadiikdqhe
     3661 tnllfsslil tqesvesgtg lisndeivfk masdilqklp adydillalk kypmsygqsm
     3721 ntvlvqemgr fnkltghvrt slqnikraik gfivmsfele diyeailtnk ipalwlrysy
     3781 pslkplgsyi hdllarlnfl qkwydegppv iywisgfyft qafltgtrqn ysrkyqipid
     3841 llifdffvlh vheiphsppe ngvyihglfl dgarfnnehm tldesfskvl yesmprmwlv
     3901 pvkkedikkr ptylcpvykt serkgvlstt ghstnfviam tlptakppeh wimrgvallc
     3961 qlse
//
LOCUS       XP_076546658            3964 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal heavy chain 7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546658
VERSION     XP_076546658.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690543.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3964
                     /product="dynein axonemal heavy chain 7 isoform X1"
                     /calculated_mol_wt=456383
     Region          672..1073
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          848..3603
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1205..1531
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          2251..2513
                     /region_name="AAA_8"
                     /note="P-loop containing dynein motor region D4;
                     pfam12780"
                     /db_xref="CDD:463701"
     Region          2899..3120
                     /region_name="AAA_9"
                     /note="ATP-binding dynein motor region; pfam12781"
                     /db_xref="CDD:463702"
     Region          3658..3960
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..3964
                     /gene="LOC117611772"
                     /coded_by="XM_076690543.1:2161..14055"
                     /db_xref="GeneID:117611772"
ORIGIN      
        1 mkcskkksvs ktykylllsq kreefrmhll nvikekkrke tmrakrkkcv qrmeeedddd
       61 ddeeeikdfv tkndrdilry yyyltqgidd dyvgsmgtdl lenilsvipi kwrqkfkecl
      121 ndlvkevkee yvlsikksvi efvignslys llnqlvalek sktigemqlp tdhavnyqwn
      181 itkleristl yrpvmrrifd ccyrefsdlk lldeselaay gesyglenfe rtvlrmchrv
      241 kenlsknwlp kiqtilsvaf ernelpsmsn svkcrrffnc ialimesqlr rlyiqtlddy
      301 idylcnkngt chgfdikvtv rhaivlfdpp fntfvetlst imnsmqeava glprvetlls
      361 niscigsdek llqvkvsdkr lercadeiek ligeekigat eqlqefekye nliygaenek
      421 itdfinstdt vehferyqnl iehyyrlygr ikvefprtsf hglfvvhrge lighilatar
      481 kikedlvtkm iqdyqrkart vgnayqhivd raltvpanss elikleefin vtktettgel
      541 ekelreiiky ivfltdysil ttielktnnf afqwyhklpe vfksheeiva skteefqrnl
      601 kkrieqflrd letyakqcne lqywgnidei yrykkkadrl enkliaamdt idkfneeevl
      661 fgwemsqypl rkatadklsp ykklydaacd fltnydtwmn smigsqdped iematgtayr
      721 tvfrlersiq epiakklaet irinieefke hmpvistlgn psmksrhweq vseivgfpik
      781 vdlsttlaki ldygladyvp kfeaiaeaat kegslekall rmhvdwedia ftvnpyrdtg
      841 tyviasvdei qlllddhltk aqtmknslyi rpfeketlew eskllllqdi mdywlqvqat
      901 wmyfepifss adiqqqmpee grrfsavdki wreimslvss dprvmsvigi dkmlerlkkc
      961 tnlldlvqkg ltayldkkrl yfprffflsn delleilset kdptrvqphl kkcfegierl
     1021 sftddmeitt mqssegekvi ledivdtvaa rgqverwlld lersmkksvr aqviasknaf
     1081 lkqvrsmwal ewpgqtilcv sklywtadvt kalssdplqn vknyihtcth qlneivklvr
     1141 gklstqnrtt lealvtldvh grdvvsglvg qrvtdatdfn wlaqlryywm nndlwttmin
     1201 tslkyayeyl gntsrlvitp ltdrcyrtlf salslhlgga pegpagtgkt ettkdlakav
     1261 akqcvvfncs dgldyialgk ffkglastga wscfdefnri dlevlsvvaq qiltiqrain
     1321 agldqllfeg teisldptca vfitmnpgya grtelpdnlk alfrpvammv pdyaliaent
     1381 lysygfyear plsvkivtay rlcseqlssq nhydygmrav ksvliaagnl kikypdeaed
     1441 ililrsikdv nlpkflkedi plfngitsdl fpgvklpepd ytdlntcvrd acselnlqct
     1501 svflqkiqqi yemmivrhgf mivgypfagk saaynvlada lklceqrnli nehavekfvi
     1561 npkavtlghl ygefdpvshe wsdgvlaisy rafatstnen rkwlifdgpi davwiesmnt
     1621 vlddnkklcl msgeiiqlsp vtslifetmd leqaspatvs rcgmiymeph tlgwdpllks
     1681 wlakvpavid ewlknfiyqs lfrrfcdpll ywlfnnnvqk icpmpesnli rsltylmdcf
     1741 vneyhdekit ksmteldlra qiegsfffsc iwaiggiltv ksresfsmlf rgfleknfpt
     1801 dlmetfelpg pieaplkpyi fvmprdslvf dyrftkegkg kwklwsdell dsppiprdip
     1861 vnqiivptve tirytslfrl lighqkpvlf vgptgtgksv ytvdfllrkn dpavnkplii
     1921 nfsaqttakq tqdtimgkld rrrkgvfgap igkrwivfvd dlsmpmkety gaqppiellr
     1981 qwldygewfd tkdktvikli dvnlmcamgp ptsggkdvtp rfkrhftvlt isefddevmt
     2041 mifskiilwh ldtrgfgkef dpcieeivla tldvykesls nllptpakch yvfnlrdfsr
     2101 viqgvllsvp etmpaltnmr rlwvheilrv fgdrliddnd ntwlvgqiri tldkrmnvim
     2161 eklfndlvps kekdskvssy flyfhspssc tvlqkqpali teqelrnlvf cdfadarvdn
     2221 rlyqevpdle rlreivenyl teynamsrkp mnlvlfrfai ehlsricrii kqprshallv
     2281 gvggsgrqsl trlashisdy dvfqievsqq ygvnawhedv kdilkrvtlt elhstflfsd
     2341 tqikeesfle disnmlnsge ipniftveev seicermrii drqrdrsvqt dgspvalfnl
     2401 fvqtvrdqlh ivivvspign nfrnrirkfp alvncctidw lqswpddall avatrflsti
     2461 eltdqeriag idmcqffhis tqnlsteffl rlnrhvyvtp tsylemintf kdllekkrrn
     2521 imdaktryer glgqldetqv qvvamsetlq slrpqlivat qdvqkmlldv nkekeevaef
     2581 ertvkedena aeiyaseaaa ikaecdadla ealpilkraq aaldtlttad vavvramkqp
     2641 pqgvrliaes vcvlkqvkpe riqqpdgsfl edywkaalrm lsdarfldsl lnfdkdnipd
     2701 nviekirkvy lsnpdfdpek ikkvstaceg lcrwvyamse ydkvskvvap kkralaqaqe
     2761 vydtamgtlr akreqlqrvq eklrtleell eqrriafqtm sdkvtdcetk lkraedligg
     2821 lggeytrwsr tavvlgeryh rltgdvvias gvvaylgpft mpfrlqqmsm wiqrctdlkv
     2881 icstdfqlrd ilgeavlirs wnifglpnda ysvdngiiit narrwpliid pqgqanrwik
     2941 nmerknnlni irltqsdysr vlenalqfgq pvllehvgee idavlepvlm ketfkqggal
     3001 cikfgdtive ysdhfrlyit tklrnphylp eiavkvtlln fmitptgled qllgivvake
     3061 rpdlesekna livqgaankq llqetedkil eilslaegnl lddeeavevl tsskdlsndi
     3121 qvkqaaaeqt eksiddarlq ytsiavystv lfftiamlin idpmyqysla wfinlfelai
     3181 dntepadtve krltdltkyf tyslytnvcr slfekdkllf sfllainllk qqgklslpqw
     3241 iflltggigi dnpyanpttw lpskqwnelc nldsvddfsg iretftqhvk ewrhlfdske
     3301 pqngaipppf hklnlfqrml ilrcirpdki ipgvqnfvee qlglqfveap pfdlpssyad
     3361 sncstplifi ltpgtdpgql llafadeqgy svsrlfylsl gqgqgpvaee tiqkavmvgn
     3421 wvvlqnchla iswmgtleni ceglipdtih sefrlwltsy psehfpssvl ensikmtnep
     3481 pkglranilr sytsdpisdp dffescsqpe yfkkllyslc ffhaivqerr kfgpigwnis
     3541 yefnetdlri svlqlhlfld efedvrfdai ryltgecnyg grvtdewdrr tlntilskfy
     3601 caelldekiy yfdetstsyy apnlrefeaf meytrdlpli tqpsvfgmne nadiikdqhe
     3661 tnllfsslil tqesvesgtg lisndeivfk masdilqklp adydillalk kypmsygqsm
     3721 ntvlvqemgr fnkltghvrt slqnikraik gfivmsfele diyeailtnk ipalwlrysy
     3781 pslkplgsyi hdllarlnfl qkwydegppv iywisgfyft qafltgtrqn ysrkyqipid
     3841 llifdffvlh vheiphsppe ngvyihglfl dgarfnnehm tldesfskvl yesmprmwlv
     3901 pvkkedikkr ptylcpvykt serkgvlstt ghstnfviam tlptakppeh wimrgvallc
     3961 qlse
//
LOCUS       XP_076546659            3964 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal heavy chain 7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546659
VERSION     XP_076546659.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690544.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3964
                     /product="dynein axonemal heavy chain 7 isoform X1"
                     /calculated_mol_wt=456383
     Region          672..1073
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          848..3603
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1205..1531
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          2251..2513
                     /region_name="AAA_8"
                     /note="P-loop containing dynein motor region D4;
                     pfam12780"
                     /db_xref="CDD:463701"
     Region          2899..3120
                     /region_name="AAA_9"
                     /note="ATP-binding dynein motor region; pfam12781"
                     /db_xref="CDD:463702"
     Region          3658..3960
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..3964
                     /gene="LOC117611772"
                     /coded_by="XM_076690544.1:223..12117"
                     /db_xref="GeneID:117611772"
ORIGIN      
        1 mkcskkksvs ktykylllsq kreefrmhll nvikekkrke tmrakrkkcv qrmeeedddd
       61 ddeeeikdfv tkndrdilry yyyltqgidd dyvgsmgtdl lenilsvipi kwrqkfkecl
      121 ndlvkevkee yvlsikksvi efvignslys llnqlvalek sktigemqlp tdhavnyqwn
      181 itkleristl yrpvmrrifd ccyrefsdlk lldeselaay gesyglenfe rtvlrmchrv
      241 kenlsknwlp kiqtilsvaf ernelpsmsn svkcrrffnc ialimesqlr rlyiqtlddy
      301 idylcnkngt chgfdikvtv rhaivlfdpp fntfvetlst imnsmqeava glprvetlls
      361 niscigsdek llqvkvsdkr lercadeiek ligeekigat eqlqefekye nliygaenek
      421 itdfinstdt vehferyqnl iehyyrlygr ikvefprtsf hglfvvhrge lighilatar
      481 kikedlvtkm iqdyqrkart vgnayqhivd raltvpanss elikleefin vtktettgel
      541 ekelreiiky ivfltdysil ttielktnnf afqwyhklpe vfksheeiva skteefqrnl
      601 kkrieqflrd letyakqcne lqywgnidei yrykkkadrl enkliaamdt idkfneeevl
      661 fgwemsqypl rkatadklsp ykklydaacd fltnydtwmn smigsqdped iematgtayr
      721 tvfrlersiq epiakklaet irinieefke hmpvistlgn psmksrhweq vseivgfpik
      781 vdlsttlaki ldygladyvp kfeaiaeaat kegslekall rmhvdwedia ftvnpyrdtg
      841 tyviasvdei qlllddhltk aqtmknslyi rpfeketlew eskllllqdi mdywlqvqat
      901 wmyfepifss adiqqqmpee grrfsavdki wreimslvss dprvmsvigi dkmlerlkkc
      961 tnlldlvqkg ltayldkkrl yfprffflsn delleilset kdptrvqphl kkcfegierl
     1021 sftddmeitt mqssegekvi ledivdtvaa rgqverwlld lersmkksvr aqviasknaf
     1081 lkqvrsmwal ewpgqtilcv sklywtadvt kalssdplqn vknyihtcth qlneivklvr
     1141 gklstqnrtt lealvtldvh grdvvsglvg qrvtdatdfn wlaqlryywm nndlwttmin
     1201 tslkyayeyl gntsrlvitp ltdrcyrtlf salslhlgga pegpagtgkt ettkdlakav
     1261 akqcvvfncs dgldyialgk ffkglastga wscfdefnri dlevlsvvaq qiltiqrain
     1321 agldqllfeg teisldptca vfitmnpgya grtelpdnlk alfrpvammv pdyaliaent
     1381 lysygfyear plsvkivtay rlcseqlssq nhydygmrav ksvliaagnl kikypdeaed
     1441 ililrsikdv nlpkflkedi plfngitsdl fpgvklpepd ytdlntcvrd acselnlqct
     1501 svflqkiqqi yemmivrhgf mivgypfagk saaynvlada lklceqrnli nehavekfvi
     1561 npkavtlghl ygefdpvshe wsdgvlaisy rafatstnen rkwlifdgpi davwiesmnt
     1621 vlddnkklcl msgeiiqlsp vtslifetmd leqaspatvs rcgmiymeph tlgwdpllks
     1681 wlakvpavid ewlknfiyqs lfrrfcdpll ywlfnnnvqk icpmpesnli rsltylmdcf
     1741 vneyhdekit ksmteldlra qiegsfffsc iwaiggiltv ksresfsmlf rgfleknfpt
     1801 dlmetfelpg pieaplkpyi fvmprdslvf dyrftkegkg kwklwsdell dsppiprdip
     1861 vnqiivptve tirytslfrl lighqkpvlf vgptgtgksv ytvdfllrkn dpavnkplii
     1921 nfsaqttakq tqdtimgkld rrrkgvfgap igkrwivfvd dlsmpmkety gaqppiellr
     1981 qwldygewfd tkdktvikli dvnlmcamgp ptsggkdvtp rfkrhftvlt isefddevmt
     2041 mifskiilwh ldtrgfgkef dpcieeivla tldvykesls nllptpakch yvfnlrdfsr
     2101 viqgvllsvp etmpaltnmr rlwvheilrv fgdrliddnd ntwlvgqiri tldkrmnvim
     2161 eklfndlvps kekdskvssy flyfhspssc tvlqkqpali teqelrnlvf cdfadarvdn
     2221 rlyqevpdle rlreivenyl teynamsrkp mnlvlfrfai ehlsricrii kqprshallv
     2281 gvggsgrqsl trlashisdy dvfqievsqq ygvnawhedv kdilkrvtlt elhstflfsd
     2341 tqikeesfle disnmlnsge ipniftveev seicermrii drqrdrsvqt dgspvalfnl
     2401 fvqtvrdqlh ivivvspign nfrnrirkfp alvncctidw lqswpddall avatrflsti
     2461 eltdqeriag idmcqffhis tqnlsteffl rlnrhvyvtp tsylemintf kdllekkrrn
     2521 imdaktryer glgqldetqv qvvamsetlq slrpqlivat qdvqkmlldv nkekeevaef
     2581 ertvkedena aeiyaseaaa ikaecdadla ealpilkraq aaldtlttad vavvramkqp
     2641 pqgvrliaes vcvlkqvkpe riqqpdgsfl edywkaalrm lsdarfldsl lnfdkdnipd
     2701 nviekirkvy lsnpdfdpek ikkvstaceg lcrwvyamse ydkvskvvap kkralaqaqe
     2761 vydtamgtlr akreqlqrvq eklrtleell eqrriafqtm sdkvtdcetk lkraedligg
     2821 lggeytrwsr tavvlgeryh rltgdvvias gvvaylgpft mpfrlqqmsm wiqrctdlkv
     2881 icstdfqlrd ilgeavlirs wnifglpnda ysvdngiiit narrwpliid pqgqanrwik
     2941 nmerknnlni irltqsdysr vlenalqfgq pvllehvgee idavlepvlm ketfkqggal
     3001 cikfgdtive ysdhfrlyit tklrnphylp eiavkvtlln fmitptgled qllgivvake
     3061 rpdlesekna livqgaankq llqetedkil eilslaegnl lddeeavevl tsskdlsndi
     3121 qvkqaaaeqt eksiddarlq ytsiavystv lfftiamlin idpmyqysla wfinlfelai
     3181 dntepadtve krltdltkyf tyslytnvcr slfekdkllf sfllainllk qqgklslpqw
     3241 iflltggigi dnpyanpttw lpskqwnelc nldsvddfsg iretftqhvk ewrhlfdske
     3301 pqngaipppf hklnlfqrml ilrcirpdki ipgvqnfvee qlglqfveap pfdlpssyad
     3361 sncstplifi ltpgtdpgql llafadeqgy svsrlfylsl gqgqgpvaee tiqkavmvgn
     3421 wvvlqnchla iswmgtleni ceglipdtih sefrlwltsy psehfpssvl ensikmtnep
     3481 pkglranilr sytsdpisdp dffescsqpe yfkkllyslc ffhaivqerr kfgpigwnis
     3541 yefnetdlri svlqlhlfld efedvrfdai ryltgecnyg grvtdewdrr tlntilskfy
     3601 caelldekiy yfdetstsyy apnlrefeaf meytrdlpli tqpsvfgmne nadiikdqhe
     3661 tnllfsslil tqesvesgtg lisndeivfk masdilqklp adydillalk kypmsygqsm
     3721 ntvlvqemgr fnkltghvrt slqnikraik gfivmsfele diyeailtnk ipalwlrysy
     3781 pslkplgsyi hdllarlnfl qkwydegppv iywisgfyft qafltgtrqn ysrkyqipid
     3841 llifdffvlh vheiphsppe ngvyihglfl dgarfnnehm tldesfskvl yesmprmwlv
     3901 pvkkedikkr ptylcpvykt serkgvlstt ghstnfviam tlptakppeh wimrgvallc
     3961 qlse
//
LOCUS       XP_034195924            3946 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal heavy chain 7 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195924
VERSION     XP_034195924.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340033.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195924.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3946
                     /product="dynein axonemal heavy chain 7 isoform X2"
                     /calculated_mol_wt=454335
     Region          672..1073
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          848..3585
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1205..1531
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          2233..2495
                     /region_name="AAA_8"
                     /note="P-loop containing dynein motor region D4;
                     pfam12780"
                     /db_xref="CDD:463701"
     Region          2881..3102
                     /region_name="AAA_9"
                     /note="ATP-binding dynein motor region; pfam12781"
                     /db_xref="CDD:463702"
     Region          3640..3942
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..3946
                     /gene="LOC117611772"
                     /coded_by="XM_034340033.2:2255..14095"
                     /db_xref="GeneID:117611772"
ORIGIN      
        1 mkcskkksvs ktykylllsq kreefrmhll nvikekkrke tmrakrkkcv qrmeeedddd
       61 ddeeeikdfv tkndrdilry yyyltqgidd dyvgsmgtdl lenilsvipi kwrqkfkecl
      121 ndlvkevkee yvlsikksvi efvignslys llnqlvalek sktigemqlp tdhavnyqwn
      181 itkleristl yrpvmrrifd ccyrefsdlk lldeselaay gesyglenfe rtvlrmchrv
      241 kenlsknwlp kiqtilsvaf ernelpsmsn svkcrrffnc ialimesqlr rlyiqtlddy
      301 idylcnkngt chgfdikvtv rhaivlfdpp fntfvetlst imnsmqeava glprvetlls
      361 niscigsdek llqvkvsdkr lercadeiek ligeekigat eqlqefekye nliygaenek
      421 itdfinstdt vehferyqnl iehyyrlygr ikvefprtsf hglfvvhrge lighilatar
      481 kikedlvtkm iqdyqrkart vgnayqhivd raltvpanss elikleefin vtktettgel
      541 ekelreiiky ivfltdysil ttielktnnf afqwyhklpe vfksheeiva skteefqrnl
      601 kkrieqflrd letyakqcne lqywgnidei yrykkkadrl enkliaamdt idkfneeevl
      661 fgwemsqypl rkatadklsp ykklydaacd fltnydtwmn smigsqdped iematgtayr
      721 tvfrlersiq epiakklaet irinieefke hmpvistlgn psmksrhweq vseivgfpik
      781 vdlsttlaki ldygladyvp kfeaiaeaat kegslekall rmhvdwedia ftvnpyrdtg
      841 tyviasvdei qlllddhltk aqtmknslyi rpfeketlew eskllllqdi mdywlqvqat
      901 wmyfepifss adiqqqmpee grrfsavdki wreimslvss dprvmsvigi dkmlerlkkc
      961 tnlldlvqkg ltayldkkrl yfprffflsn delleilset kdptrvqphl kkcfegierl
     1021 sftddmeitt mqssegekvi ledivdtvaa rgqverwlld lersmkksvr aqviasknaf
     1081 lkqvrsmwal ewpgqtilcv sklywtadvt kalssdplqn vknyihtcth qlneivklvr
     1141 gklstqnrtt lealvtldvh grdvvsglvg qrvtdatdfn wlaqlryywm nndlwttmin
     1201 tslkyayeyl gntsrlvitp ltdrcyrtlf salslhlgga pegpagtgkt ettkdlakav
     1261 akqcvvfncs dgldyialgk ffkglastga wscfdefnri dlevlsvvaq qiltiqrain
     1321 agldqllfeg teisldptca vfitmnpgya grtelpdnlk alfrpvammv pdyaliaent
     1381 lysygfyear plsvkivtay rlcseqlssq nhydygmrav ksvliaagnl kikypdeaed
     1441 ililrsikdv nlpkflkedi plfngitsdl fpgvklpepd ytdlntcvrd acselnlqct
     1501 svflqkiqqi yemmivrhgf mivgypfagk saaynvlada lklceqrnli nehavekfvi
     1561 npkavtlghl ygefdpvshe wsdgvlaisy rafatstnen rkwlifdgpi davwiesmnt
     1621 vlddnkklcl msgeiiqlsp vtslifetmd leqaspatvs rcgmiymeph tlgwdpllks
     1681 wlakvpavid ewlknfiyqs lfrrfcdpll ywlfnnnvqk icpmpesnli rsltylmdcf
     1741 vneyhdekit ksmteldlra qiegsfffsc iwaiggiltv ksresfsmlf rgfleknfpt
     1801 dlmetfelpg pieaplkpyi fvmprdslvf dyrftkegkg kwklwsdell dsppiprdip
     1861 vnqiivptve tirytslfrl lighqkpvlf vgptgtgksv ytvdfllrkn dpavnkplii
     1921 nfsaqttakq tqdtimgkld rrrkgvfgap igkrwivfvd dlsmpmkety gaqppiellr
     1981 qwldygewfd tkdktvikli dvnlmcamgp ptsggkdvtp rfkrhftvlt isefddevmt
     2041 mifskiilwh ldtrgfgkef dpcieeivla tldvykesls nllptpakch yvfnlrdfsr
     2101 viqgvllsvp etmpaltnmr rlwvheilrv fgdrliddnd ntwlvgqiri tldkrmnvim
     2161 eklfndlvps kekdskkqpa liteqelrnl vfcdfadarv dnrlyqevpd lerlreiven
     2221 ylteynamsr kpmnlvlfrf aiehlsricr iikqprshal lvgvggsgrq sltrlashis
     2281 dydvfqievs qqygvnawhe dvkdilkrvt ltelhstflf sdtqikeesf ledisnmlns
     2341 geipniftve evseicermr iidrqrdrsv qtdgspvalf nlfvqtvrdq lhivivvspi
     2401 gnnfrnrirk fpalvnccti dwlqswpdda llavatrfls tieltdqeri agidmcqffh
     2461 istqnlstef flrlnrhvyv tptsylemin tfkdllekkr rnimdaktry erglgqldet
     2521 qvqvvamset lqslrpqliv atqdvqkmll dvnkekeeva efertvkede naaeiyasea
     2581 aaikaecdad laealpilkr aqaaldtltt advavvramk qppqgvrlia esvcvlkqvk
     2641 periqqpdgs fledywkaal rmlsdarfld sllnfdkdni pdnviekirk vylsnpdfdp
     2701 ekikkvstac eglcrwvyam seydkvskvv apkkralaqa qevydtamgt lrakreqlqr
     2761 vqeklrtlee lleqrriafq tmsdkvtdce tklkraedli gglggeytrw srtavvlger
     2821 yhrltgdvvi asgvvaylgp ftmpfrlqqm smwiqrctdl kvicstdfql rdilgeavli
     2881 rswnifglpn daysvdngii itnarrwpli idpqgqanrw iknmerknnl niirltqsdy
     2941 srvlenalqf gqpvllehvg eeidavlepv lmketfkqgg alcikfgdti veysdhfrly
     3001 ittklrnphy lpeiavkvtl lnfmitptgl edqllgivva kerpdlesek nalivqgaan
     3061 kqllqetedk ileilslaeg nllddeeave vltsskdlsn diqvkqaaae qteksiddar
     3121 lqytsiavys tvlfftiaml inidpmyqys lawfinlfel aidntepadt vekrltdltk
     3181 yftyslytnv crslfekdkl lfsfllainl lkqqgklslp qwiflltggi gidnpyanpt
     3241 twlpskqwne lcnldsvddf sgiretftqh vkewrhlfds kepqngaipp pfhklnlfqr
     3301 mlilrcirpd kiipgvqnfv eeqlglqfve appfdlpssy adsncstpli filtpgtdpg
     3361 qlllafadeq gysvsrlfyl slgqgqgpva eetiqkavmv gnwvvlqnch laiswmgtle
     3421 niceglipdt ihsefrlwlt sypsehfpss vlensikmtn eppkglrani lrsytsdpis
     3481 dpdffescsq peyfkkllys lcffhaivqe rrkfgpigwn isyefnetdl risvlqlhlf
     3541 ldefedvrfd airyltgecn yggrvtdewd rrtlntilsk fycaelldek iyyfdetsts
     3601 yyapnlrefe afmeytrdlp litqpsvfgm nenadiikdq hetnllfssl iltqesvesg
     3661 tglisndeiv fkmasdilqk lpadydilla lkkypmsygq smntvlvqem grfnkltghv
     3721 rtslqnikra ikgfivmsfe lediyeailt nkipalwlry sypslkplgs yihdllarln
     3781 flqkwydegp pviywisgfy ftqafltgtr qnysrkyqip idllifdffv lhvheiphsp
     3841 pengvyihgl fldgarfnne hmtldesfsk vlyesmprmw lvpvkkedik krptylcpvy
     3901 ktserkgvls ttghstnfvi amtlptakpp ehwimrgval lcqlse
//
LOCUS       XP_034195925            3400 aa            linear   INV 03-SEP-2025
DEFINITION  rabconnectin-3 alpha isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195925
VERSION     XP_034195925.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195925.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3400
                     /product="rabconnectin-3 alpha isoform X1"
                     /calculated_mol_wt=379439
     Region          <192..>309
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1371..2059
                     /region_name="Rav1p_C"
                     /note="RAVE protein 1 C terminal; pfam12234"
                     /db_xref="CDD:432413"
     Region          3126..3395
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(3142,3146,3152..3153,3167..3168,3186,3190,
                     3196..3197,3213..3214,3231,3239,3245..3246,3262,3279,3284,
                     3290..3291,3303..3304,3322,3326,3332..3333,3345,3347,3381,
                     3387..3388)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          3173..3213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3218..3260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3267..3302
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3308..3356
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3400
                     /gene="Rbcn-3A"
                     /coded_by="XM_034340034.2:56..10258"
                     /db_xref="GeneID:117611773"
ORIGIN      
        1 mnchqilsga cnagdrcyav gsvegisfta yaagcnivil asnfervqii pgavhnyiri
       61 scldcstdtg kiaaayenqv cifeptplih stcshqleyr wvqtgslttd snitslswnl
      121 egtrlltgge llqlwhqniv pfqkehttke ntlrtqegvv tgreentsii lpesstvtfs
      181 iggdaespap sdtatgnepg awncvwkcrt atpvhlmsfs pdgtlfattg fndrlvkiwf
      241 enkqlftsrn idhtnasqsm gndsysfvyi ahpravthls wrktskhmpk gavsnmlvts
      301 crdnicrvwa etippevegl anmsqfegsd rhghhgkhrh hnmhkhrfmq rlkhmktcfh
      361 irrhakqqhq aghataptlp tlpstysvhd fhnnyqgtgq ypgmhfhlaa sinaetdipl
      421 vpslitgdpe repnfilhwl nnkemyftmq aesflqelar ivvekeeglh qqdaerpehd
      481 seeeglkkgv rlqpiqkpgg gtirstsqee hssdehhtth tvsshhslph svhsnlshqs
      541 lsnttsinsi atdvtssmnh apdsldtkie tllrdwhhnp dllfsihpid gsfliwhiew
      601 ldeyhpgsfr qaqisfstri pnafplgdas tmshsvslys hntggpllni revaksstks
      661 sneateiatp lpslieqdee qstltskagq ellknlenss dpnqnankdk ngqlengktn
      721 qetyndllth pspivsmvtk hsngtlnlwq ltfadktkfs qvlsighasr asghrfrvnd
      781 itchpvlpll vttshhnipe sgsqcrnsds setidsksdp nkmtkelspt gfcselilwr
      841 vdtvgplsks ggvselarin speisafsnv awiptllpst tlgnlsnsps acfvasdgec
      901 lrvyqavida rtllaevsss errsrmmdsm aslstdissd dgirhsihdk ikivsqqsta
      961 rpgcviqlda isdathdwqn tqflhvfqeq litgdrneek qagvdtsind lglmestlda
     1021 mvdlqqsaif eepfyivvle rtqqgttvhm wrlviasqpe ttglsgsmmy vpdshlvqde
     1081 eeegtpsryh qegrrsrrps qsrresqges dtffqrranq nshvlitttk vctqelplpd
     1141 gvevihaapa aghlssssiy pacfapyiiv tacsdstirf wkckvtkdpp ddklqyewce
     1201 wemirkdqes tiditgqpln vsaaysgria caykygksft rptksdpdsr yvnlcvaiye
     1261 cestggsewi ledtihlkni hlpripvdqh ldlsylydsr flqkkqrltq vlqtlshedv
     1321 rlprngenge stksnaglla vpsfstlqsl rksiiengnt cpltqkhlvq ldwvskedgs
     1381 hiltvavgsk imlftpvcsd laqanmkamk esqsnnrpil rkasslaqpq fvdeirwmkl
     1441 rkielttadg lpplpmqisw vrdgilvvgm dsemhvysqw kpnpkkecfh snlqhqesde
     1501 fqasrnlrde dlrtlanets qrrlanvssm phlsrvssin ltmldakkkr gmqsenisfd
     1561 ympdyglfea sriacpvlpq yhpkqlmell nsgkirwvka ilahlvrcig sscslradde
     1621 slmkqrggaw srsrtmsvsy vgttsplesr gsttqipeel mldyaeitsi splplwtlli
     1681 adketnlphq hktedkhdyn elfdsnldeg eslddmleed ydcsrqkdrr ssvperqgis
     1741 hfgprqgrll srllththlp glssldqmhl laladtvstc nvdfaerfai daaknaiake
     1801 nltgipdget istdslddcg lrfllamkhy nylirclpla qrahfqkqgv ssnnlvwafh
     1861 seteeellgl ipsyakgqpk wsvlkelgig wwirsntvlk rcvekiakaa yqqkqeplda
     1921 alyylamkkk nlvwglfrnk rddkmtsffa nnftevrwrk aaltnafall gkqrfehaaa
     1981 ffllagalrd aievcldkln diqlamviar lyeddttspn mrrllyeeil gydkdgqcqd
     2041 mnkahpdpfl rsmalwilkd yagslntlll tnvgtlhpqy ndesdkpegt tvsanpnvfn
     2101 fyvylrthpl lirqyiasta qdkkkghsvv isgfsygtdt ktqpdkqlml edsitplerq
     2161 lyfttahahf kagcpalale vlsklpskvm dtncedspsl lnspskartq dfqidtgiit
     2221 wnedhskqts ndtgaavldw sepvtkqted elvlnwedde veqgdsdspp lsmkldkket
     2281 ddnladaekd ekakaagqld imaqqlkfva clkilmeels tlatgfevdg gqlryqlyvw
     2341 lerevealrq lcsysvnadg dannapeyeg gmvddvqsyr pgeqptlhei lvadkldfea
     2401 kvqravkrkk wlkanetllr tllsycslhg asggglasvr melvlllqel qqektqqqll
     2461 splpfpttlp llsasvacnk tvvadpvrhl qslahdmlqt lvelrnppmp trnthycevf
     2521 imrdlavals aciyqslcds dtfvmkhhqp dsfpavaeie tfsgghlvas nrhhrrfstd
     2581 dgvcitttps kwpgvtnlra llarekdedt pklnvllcea fvatymslfv yamwscdshi
     2641 lyrlvgqhfd nstwsclfgg gvkkllrvas tssqasgsgt tekvtsgsid agsseiqtaa
     2701 gamwntmasl tkqrvklnmk llgpftgqqp nmkedkptyr eqfvspqmsm isyflmkpqi
     2761 dseyadeidy dssdsavsdl dsseeeedvf dtnskpktri kdntehsnpn syswcvmrla
     2821 iarilqqqlq dflnvagiem qelpvnspli hgtlavvaqw qetlreelds kgpplvnyip
     2881 gcapdptptp gkpaihkyrs llekgntpfn trlasaapan rlwcylvrqe svqdifirav
     2941 fgkrrclssi lennqntidn lnrgtaedkg sdsgttslpe pvriihkeqd sisafclnqv
     3001 sqglmalatp revqemnisl llelpswled ecefdiinln kqpepepvqp tsflviqtaa
     3061 drpllaqspq pnspqpqsgi asqsgrgasv mkgmpafpgs hdlrfcqfva drskhllqpi
     3121 lkhkidgirr isshpllply ltgsqdgsvs lwewghqtav atprppgtfa kvtrvrfsqh
     3181 gnkfgvadsd ghlslfqvac regsarpffn yqchskvtad fvflgacsli ataghgsegr
     3241 nvalwdtllp qnksliqgft chdqgassli lapqhqllis ggkkgdinif dvrqrqqrhr
     3301 fqahesaikc laldpheeff vsgagdgdik iwgltvhsll ysfpaehprs sffknigqnv
     3361 fqgvtqlhvd sagrlfscga dgsmkvrqlp erdcvvhtly
//
LOCUS       XP_034195926            3396 aa            linear   INV 03-SEP-2025
DEFINITION  rabconnectin-3 alpha isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195926
VERSION     XP_034195926.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340035.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195926.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3396
                     /product="rabconnectin-3 alpha isoform X2"
                     /calculated_mol_wt=378950
     Region          <192..>309
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1371..2059
                     /region_name="Rav1p_C"
                     /note="RAVE protein 1 C terminal; pfam12234"
                     /db_xref="CDD:432413"
     Region          3126..3391
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(3142,3146,3152..3153,3167..3168,3186,3190,
                     3196..3197,3213..3214,3231,3239,3245..3246,3262,3279,3284,
                     3290..3291,3303..3304,3322,3326,3332..3333,3345,3347,3377,
                     3383..3384)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          3173..3213
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3218..3260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3267..3302
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3308..3340
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3396
                     /gene="Rbcn-3A"
                     /coded_by="XM_034340035.2:56..10246"
                     /db_xref="GeneID:117611773"
ORIGIN      
        1 mnchqilsga cnagdrcyav gsvegisfta yaagcnivil asnfervqii pgavhnyiri
       61 scldcstdtg kiaaayenqv cifeptplih stcshqleyr wvqtgslttd snitslswnl
      121 egtrlltgge llqlwhqniv pfqkehttke ntlrtqegvv tgreentsii lpesstvtfs
      181 iggdaespap sdtatgnepg awncvwkcrt atpvhlmsfs pdgtlfattg fndrlvkiwf
      241 enkqlftsrn idhtnasqsm gndsysfvyi ahpravthls wrktskhmpk gavsnmlvts
      301 crdnicrvwa etippevegl anmsqfegsd rhghhgkhrh hnmhkhrfmq rlkhmktcfh
      361 irrhakqqhq aghataptlp tlpstysvhd fhnnyqgtgq ypgmhfhlaa sinaetdipl
      421 vpslitgdpe repnfilhwl nnkemyftmq aesflqelar ivvekeeglh qqdaerpehd
      481 seeeglkkgv rlqpiqkpgg gtirstsqee hssdehhtth tvsshhslph svhsnlshqs
      541 lsnttsinsi atdvtssmnh apdsldtkie tllrdwhhnp dllfsihpid gsfliwhiew
      601 ldeyhpgsfr qaqisfstri pnafplgdas tmshsvslys hntggpllni revaksstks
      661 sneateiatp lpslieqdee qstltskagq ellknlenss dpnqnankdk ngqlengktn
      721 qetyndllth pspivsmvtk hsngtlnlwq ltfadktkfs qvlsighasr asghrfrvnd
      781 itchpvlpll vttshhnipe sgsqcrnsds setidsksdp nkmtkelspt gfcselilwr
      841 vdtvgplsks ggvselarin speisafsnv awiptllpst tlgnlsnsps acfvasdgec
      901 lrvyqavida rtllaevsss errsrmmdsm aslstdissd dgirhsihdk ikivsqqsta
      961 rpgcviqlda isdathdwqn tqflhvfqeq litgdrneek qagvdtsind lglmestlda
     1021 mvdlqqsaif eepfyivvle rtqqgttvhm wrlviasqpe ttglsgsmmy vpdshlvqde
     1081 eeegtpsryh qegrrsrrps qsrresqges dtffqrranq nshvlitttk vctqelplpd
     1141 gvevihaapa aghlssssiy pacfapyiiv tacsdstirf wkckvtkdpp ddklqyewce
     1201 wemirkdqes tiditgqpln vsaaysgria caykygksft rptksdpdsr yvnlcvaiye
     1261 cestggsewi ledtihlkni hlpripvdqh ldlsylydsr flqkkqrltq vlqtlshedv
     1321 rlprngenge stksnaglla vpsfstlqsl rksiiengnt cpltqkhlvq ldwvskedgs
     1381 hiltvavgsk imlftpvcsd laqanmkamk esqsnnrpil rkasslaqpq fvdeirwmkl
     1441 rkielttadg lpplpmqisw vrdgilvvgm dsemhvysqw kpnpkkecfh snlqhqesde
     1501 fqasrnlrde dlrtlanets qrrlanvssm phlsrvssin ltmldakkkr gmqsenisfd
     1561 ympdyglfea sriacpvlpq yhpkqlmell nsgkirwvka ilahlvrcig sscslradde
     1621 slmkqrggaw srsrtmsvsy vgttsplesr gsttqipeel mldyaeitsi splplwtlli
     1681 adketnlphq hktedkhdyn elfdsnldeg eslddmleed ydcsrqkdrr ssvperqgis
     1741 hfgprqgrll srllththlp glssldqmhl laladtvstc nvdfaerfai daaknaiake
     1801 nltgipdget istdslddcg lrfllamkhy nylirclpla qrahfqkqgv ssnnlvwafh
     1861 seteeellgl ipsyakgqpk wsvlkelgig wwirsntvlk rcvekiakaa yqqkqeplda
     1921 alyylamkkk nlvwglfrnk rddkmtsffa nnftevrwrk aaltnafall gkqrfehaaa
     1981 ffllagalrd aievcldkln diqlamviar lyeddttspn mrrllyeeil gydkdgqcqd
     2041 mnkahpdpfl rsmalwilkd yagslntlll tnvgtlhpqy ndesdkpegt tvsanpnvfn
     2101 fyvylrthpl lirqyiasta qdkkkghsvv isgfsygtdt ktqpdkqlml edsitplerq
     2161 lyfttahahf kagcpalale vlsklpskvm dtncedspsl lnspskartq dfqidtgiit
     2221 wnedhskqts ndtgaavldw sepvtkqted elvlnwedde veqgdsdspp lsmkldkket
     2281 ddnladaekd ekakaagqld imaqqlkfva clkilmeels tlatgfevdg gqlryqlyvw
     2341 lerevealrq lcsysvnadg dannapeyeg gmvddvqsyr pgeqptlhei lvadkldfea
     2401 kvqravkrkk wlkanetllr tllsycslhg asggglasvr melvlllqel qqektqqqll
     2461 splpfpttlp llsasvacnk tvvadpvrhl qslahdmlqt lvelrnppmp trnthycevf
     2521 imrdlavals aciyqslcds dtfvmkhhqp dsfpavaeie tfsgghlvas nrhhrrfstd
     2581 dgvcitttps kwpgvtnlra llarekdedt pklnvllcea fvatymslfv yamwscdshi
     2641 lyrlvgqhfd nstwsclfgg gvkkllrvas tssqasgsgt tekvtsgsid agsseiqtaa
     2701 gamwntmasl tkqrvklnmk llgpftgqqp nmkedkptyr eqfvspqmsm isyflmkpqi
     2761 dseyadeidy dssdsavsdl dsseeeedvf dtnskpktri kdntehsnpn syswcvmrla
     2821 iarilqqqlq dflnvagiem qelpvnspli hgtlavvaqw qetlreelds kgpplvnyip
     2881 gcapdptptp gkpaihkyrs llekgntpfn trlasaapan rlwcylvrqe svqdifirav
     2941 fgkrrclssi lennqntidn lnrgtaedkg sdsgttslpe pvriihkeqd sisafclnqv
     3001 sqglmalatp revqemnisl llelpswled ecefdiinln kqpepepvqp tsflviqtaa
     3061 drpllaqspq pnspqpqsgi asqsgrgasv mkgmpafpgs hdlrfcqfva drskhllqpi
     3121 lkhkidgirr isshpllply ltgsqdgsvs lwewghqtav atprppgtfa kvtrvrfsqh
     3181 gnkfgvadsd ghlslfqvac regsarpffn yqchskvtad fvflgacsli ataghgsegr
     3241 nvalwdtllp qnksliqgft chdqgassli lapqhqllis ggkkgdinif dvrqrqqrhr
     3301 fqahesaikc laldpheeff vsgagdgdik iwgltvhsll ysfpaehprs sffknigqgv
     3361 tqlhvdsagr lfscgadgsm kvrqlperdc vvhtly
//
LOCUS       XP_034195927            3373 aa            linear   INV 03-SEP-2025
DEFINITION  rabconnectin-3 alpha isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195927
VERSION     XP_034195927.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340036.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195927.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3373
                     /product="rabconnectin-3 alpha isoform X3"
                     /calculated_mol_wt=376455
     Region          61..98
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <110..>282
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          113..153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          187..233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          249..282
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1344..2032
                     /region_name="Rav1p_C"
                     /note="RAVE protein 1 C terminal; pfam12234"
                     /db_xref="CDD:432413"
     Region          3099..3368
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(3115,3119,3125..3126,3140..3141,3159,3163,
                     3169..3170,3186..3187,3204,3212,3218..3219,3235,3252,3257,
                     3263..3264,3276..3277,3295,3299,3305..3306,3318,3320,3354,
                     3360..3361)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          3146..3186
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3191..3233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3240..3275
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3281..3329
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3373
                     /gene="Rbcn-3A"
                     /coded_by="XM_034340036.2:56..10177"
                     /db_xref="GeneID:117611773"
ORIGIN      
        1 mnchqilsga cnagdrcyav gsvegisfta yaagcnivil asnfervqii pgavhnyiri
       61 scldcstdtg kiaaayenqv cifeptplih stcshqleyr wvqtgslttd snitslswnl
      121 egtrlltgge llqlwhqniv pfqkehtstv tfsiggdaes papsdtatgn epgawncvwk
      181 crtatpvhlm sfspdgtlfa ttgfndrlvk iwfenkqlft srnidhtnas qsmgndsysf
      241 vyiahpravt hlswrktskh mpkgavsnml vtscrdnicr vwaetippev eglanmsqfe
      301 gsdrhghhgk hrhhnmhkhr fmqrlkhmkt cfhirrhakq qhqaghatap tlptlpstys
      361 vhdfhnnyqg tgqypgmhfh laasinaetd iplvpslitg dperepnfil hwlnnkemyf
      421 tmqaesflqe larivvekee glhqqdaerp ehdseeeglk kgvrlqpiqk pgggtirsts
      481 qeehssdehh tthtvsshhs lphsvhsnls hqslsnttsi nsiatdvtss mnhapdsldt
      541 kietllrdwh hnpdllfsih pidgsfliwh iewldeyhpg sfrqaqisfs tripnafplg
      601 dastmshsvs lyshntggpl lnirevakss tkssneatei atplpslieq deeqstltsk
      661 agqellknle nssdpnqnan kdkngqleng ktnqetyndl lthpspivsm vtkhsngtln
      721 lwqltfadkt kfsqvlsigh asrasghrfr vnditchpvl pllvttshhn ipesgsqcrn
      781 sdssetidsk sdpnkmtkel sptgfcseli lwrvdtvgpl sksggvsela rinspeisaf
      841 snvawiptll psttlgnlsn spsacfvasd geclrvyqav idartllaev ssserrsrmm
      901 dsmaslstdi ssddgirhsi hdkikivsqq starpgcviq ldaisdathd wqntqflhvf
      961 qeqlitgdrn eekqagvdts indlglmest ldamvdlqqs aifeepfyiv vlertqqgtt
     1021 vhmwrlvias qpettglsgs mmyvpdshlv qdeeeegtps ryhqegrrsr rpsqsrresq
     1081 gesdtffqrr anqnshvlit ttkvctqelp lpdgveviha apaaghlsss siypacfapy
     1141 iivtacsdst irfwkckvtk dppddklqye wcewemirkd qestiditgq plnvsaaysg
     1201 riacaykygk sftrptksdp dsryvnlcva iyecestggs ewiledtihl knihlpripv
     1261 dqhldlsyly dsrflqkkqr ltqvlqtlsh edvrlprnge ngestksnag llavpsfstl
     1321 qslrksiien gntcpltqkh lvqldwvske dgshiltvav gskimlftpv csdlaqanmk
     1381 amkesqsnnr pilrkassla qpqfvdeirw mklrkieltt adglpplpmq iswvrdgilv
     1441 vgmdsemhvy sqwkpnpkke cfhsnlqhqe sdefqasrnl rdedlrtlan etsqrrlanv
     1501 ssmphlsrvs sinltmldak kkrgmqseni sfdympdygl feasriacpv lpqyhpkqlm
     1561 ellnsgkirw vkailahlvr cigsscslra ddeslmkqrg gawsrsrtms vsyvgttspl
     1621 esrgsttqip eelmldyaei tsisplplwt lliadketnl phqhktedkh dynelfdsnl
     1681 degeslddml eedydcsrqk drrssvperq gishfgprqg rllsrlltht hlpglssldq
     1741 mhllaladtv stcnvdfaer faidaaknai akenltgipd getistdsld dcglrfllam
     1801 khynylircl plaqrahfqk qgvssnnlvw afhseteeel lglipsyakg qpkwsvlkel
     1861 gigwwirsnt vlkrcvekia kaayqqkqep ldaalyylam kkknlvwglf rnkrddkmts
     1921 ffannftevr wrkaaltnaf allgkqrfeh aaaffllaga lrdaievcld klndiqlamv
     1981 iarlyeddtt spnmrrllye eilgydkdgq cqdmnkahpd pflrsmalwi lkdyagslnt
     2041 llltnvgtlh pqyndesdkp egttvsanpn vfnfyvylrt hpllirqyia staqdkkkgh
     2101 svvisgfsyg tdtktqpdkq lmledsitpl erqlyfttah ahfkagcpal alevlsklps
     2161 kvmdtnceds psllnspska rtqdfqidtg iitwnedhsk qtsndtgaav ldwsepvtkq
     2221 tedelvlnwe ddeveqgdsd spplsmkldk ketddnlada ekdekakaag qldimaqqlk
     2281 fvaclkilme elstlatgfe vdggqlryql yvwlerevea lrqlcsysvn adgdannape
     2341 yeggmvddvq syrpgeqptl heilvadkld feakvqravk rkkwlkanet llrtllsycs
     2401 lhgasgggla svrmelvlll qelqqektqq qllsplpfpt tlpllsasva cnktvvadpv
     2461 rhlqslahdm lqtlvelrnp pmptrnthyc evfimrdlav alsaciyqsl cdsdtfvmkh
     2521 hqpdsfpava eietfsgghl vasnrhhrrf stddgvcitt tpskwpgvtn lrallarekd
     2581 edtpklnvll ceafvatyms lfvyamwscd shilyrlvgq hfdnstwscl fgggvkkllr
     2641 vastssqasg sgttekvtsg sidagsseiq taagamwntm asltkqrvkl nmkllgpftg
     2701 qqpnmkedkp tyreqfvspq msmisyflmk pqidseyade idydssdsav sdldsseeee
     2761 dvfdtnskpk trikdntehs npnsyswcvm rlaiarilqq qlqdflnvag iemqelpvns
     2821 plihgtlavv aqwqetlree ldskgpplvn yipgcapdpt ptpgkpaihk yrsllekgnt
     2881 pfntrlasaa panrlwcylv rqesvqdifi ravfgkrrcl ssilennqnt idnlnrgtae
     2941 dkgsdsgtts lpepvriihk eqdsisafcl nqvsqglmal atprevqemn islllelpsw
     3001 ledecefdii nlnkqpepep vqptsflviq taadrpllaq spqpnspqpq sgiasqsgrg
     3061 asvmkgmpaf pgshdlrfcq fvadrskhll qpilkhkidg irrisshpll plyltgsqdg
     3121 svslwewghq tavatprppg tfakvtrvrf sqhgnkfgva dsdghlslfq vacregsarp
     3181 ffnyqchskv tadfvflgac sliataghgs egrnvalwdt llpqnksliq gftchdqgas
     3241 slilapqhql lisggkkgdi nifdvrqrqq rhrfqahesa ikclaldphe effvsgagdg
     3301 dikiwgltvh sllysfpaeh prssffknig qnvfqgvtql hvdsagrlfs cgadgsmkvr
     3361 qlperdcvvh tly
//
LOCUS       XP_034195928            3371 aa            linear   INV 03-SEP-2025
DEFINITION  rabconnectin-3 alpha isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195928
VERSION     XP_034195928.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340037.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195928.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3371
                     /product="rabconnectin-3 alpha isoform X4"
                     /calculated_mol_wt=376142
     Region          <192..>309
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1371..2059
                     /region_name="Rav1p_C"
                     /note="RAVE protein 1 C terminal; pfam12234"
                     /db_xref="CDD:432413"
     Region          3090..3366
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(3094,3113,3117,3123..3124,3138..3139,3157,3161,
                     3167..3168,3184..3185,3202,3210,3216..3217,3233,3250,3255,
                     3261..3262,3274..3275,3293,3297,3303..3304,3316,3318,3352,
                     3358..3359)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          3144..3184
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3189..3231
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3238..3273
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3279..3327
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3371
                     /gene="Rbcn-3A"
                     /coded_by="XM_034340037.2:56..10171"
                     /db_xref="GeneID:117611773"
ORIGIN      
        1 mnchqilsga cnagdrcyav gsvegisfta yaagcnivil asnfervqii pgavhnyiri
       61 scldcstdtg kiaaayenqv cifeptplih stcshqleyr wvqtgslttd snitslswnl
      121 egtrlltgge llqlwhqniv pfqkehttke ntlrtqegvv tgreentsii lpesstvtfs
      181 iggdaespap sdtatgnepg awncvwkcrt atpvhlmsfs pdgtlfattg fndrlvkiwf
      241 enkqlftsrn idhtnasqsm gndsysfvyi ahpravthls wrktskhmpk gavsnmlvts
      301 crdnicrvwa etippevegl anmsqfegsd rhghhgkhrh hnmhkhrfmq rlkhmktcfh
      361 irrhakqqhq aghataptlp tlpstysvhd fhnnyqgtgq ypgmhfhlaa sinaetdipl
      421 vpslitgdpe repnfilhwl nnkemyftmq aesflqelar ivvekeeglh qqdaerpehd
      481 seeeglkkgv rlqpiqkpgg gtirstsqee hssdehhtth tvsshhslph svhsnlshqs
      541 lsnttsinsi atdvtssmnh apdsldtkie tllrdwhhnp dllfsihpid gsfliwhiew
      601 ldeyhpgsfr qaqisfstri pnafplgdas tmshsvslys hntggpllni revaksstks
      661 sneateiatp lpslieqdee qstltskagq ellknlenss dpnqnankdk ngqlengktn
      721 qetyndllth pspivsmvtk hsngtlnlwq ltfadktkfs qvlsighasr asghrfrvnd
      781 itchpvlpll vttshhnipe sgsqcrnsds setidsksdp nkmtkelspt gfcselilwr
      841 vdtvgplsks ggvselarin speisafsnv awiptllpst tlgnlsnsps acfvasdgec
      901 lrvyqavida rtllaevsss errsrmmdsm aslstdissd dgirhsihdk ikivsqqsta
      961 rpgcviqlda isdathdwqn tqflhvfqeq litgdrneek qagvdtsind lglmestlda
     1021 mvdlqqsaif eepfyivvle rtqqgttvhm wrlviasqpe ttglsgsmmy vpdshlvqde
     1081 eeegtpsryh qegrrsrrps qsrresqges dtffqrranq nshvlitttk vctqelplpd
     1141 gvevihaapa aghlssssiy pacfapyiiv tacsdstirf wkckvtkdpp ddklqyewce
     1201 wemirkdqes tiditgqpln vsaaysgria caykygksft rptksdpdsr yvnlcvaiye
     1261 cestggsewi ledtihlkni hlpripvdqh ldlsylydsr flqkkqrltq vlqtlshedv
     1321 rlprngenge stksnaglla vpsfstlqsl rksiiengnt cpltqkhlvq ldwvskedgs
     1381 hiltvavgsk imlftpvcsd laqanmkamk esqsnnrpil rkasslaqpq fvdeirwmkl
     1441 rkielttadg lpplpmqisw vrdgilvvgm dsemhvysqw kpnpkkecfh snlqhqesde
     1501 fqasrnlrde dlrtlanets qrrlanvssm phlsrvssin ltmldakkkr gmqsenisfd
     1561 ympdyglfea sriacpvlpq yhpkqlmell nsgkirwvka ilahlvrcig sscslradde
     1621 slmkqrggaw srsrtmsvsy vgttsplesr gsttqipeel mldyaeitsi splplwtlli
     1681 adketnlphq hktedkhdyn elfdsnldeg eslddmleed ydcsrqkdrr ssvperqgis
     1741 hfgprqgrll srllththlp glssldqmhl laladtvstc nvdfaerfai daaknaiake
     1801 nltgipdget istdslddcg lrfllamkhy nylirclpla qrahfqkqgv ssnnlvwafh
     1861 seteeellgl ipsyakgqpk wsvlkelgig wwirsntvlk rcvekiakaa yqqkqeplda
     1921 alyylamkkk nlvwglfrnk rddkmtsffa nnftevrwrk aaltnafall gkqrfehaaa
     1981 ffllagalrd aievcldkln diqlamviar lyeddttspn mrrllyeeil gydkdgqcqd
     2041 mnkahpdpfl rsmalwilkd yagslntlll tnvgtlhpqy ndesdkpegt tvsanpnvfn
     2101 fyvylrthpl lirqyiasta qdkkkghsvv isgfsygtdt ktqpdkqlml edsitplerq
     2161 lyfttahahf kagcpalale vlsklpskvm dtncedspsl lnspskartq dfqidtgiit
     2221 wnedhskqts ndtgaavldw sepvtkqted elvlnwedde veqgdsdspp lsmkldkket
     2281 ddnladaekd ekakaagqld imaqqlkfva clkilmeels tlatgfevdg gqlryqlyvw
     2341 lerevealrq lcsysvnadg dannapeyeg gmvddvqsyr pgeqptlhei lvadkldfea
     2401 kvqravkrkk wlkanetllr tllsycslhg asggglasvr melvlllqel qqektqqqll
     2461 splpfpttlp llsasvacnk tvvadpvrhl qslahdmlqt lvelrnppmp trnthycevf
     2521 imrdlavals aciyqslcds dtfvmkhhqp dsfpavaeie tfsgghlvas nrhhrrfstd
     2581 dgvcitttps kwpgvtnlra llarekdedt pklnvllcea fvatymslfv yamwscdshi
     2641 lyrlvgqhfd nstwsclfgg gvkkllrvas tssqasgsgt tekvtsgsid agsseiqtaa
     2701 gamwntmasl tkqrvklnmk llgpftgqqp nmkedkptyr eqfvspqmsm isyflmkpqi
     2761 dseyadeidy dssdsavsdl dsseeeedvf dtnskpktri kdntehsnpn syswcvmrla
     2821 iarilqqqlq dflnvagiem qelpvnspli hgtlavvaqw qetlreelds kgpplvnyip
     2881 gcapdptptp gkpaihkyrs llekgntpfn trlasaapan rlwcylvrqe svqdifirav
     2941 fgkrrclssi lennqntidn lnrgtaedkg sdsgttslpe pvriihkeqd sisafclnqv
     3001 sqglmalatp revqemnisl llelpswled ecefdiinln kqpepepvqp tsflviqtaa
     3061 drpllaqspq pnspqpqsgi asqsgrgasv ilkhkidgir risshpllpl yltgsqdgsv
     3121 slwewghqta vatprppgtf akvtrvrfsq hgnkfgvads dghlslfqva cregsarpff
     3181 nyqchskvta dfvflgacsl iataghgseg rnvalwdtll pqnksliqgf tchdqgassl
     3241 ilapqhqlli sggkkgdini fdvrqrqqrh rfqahesaik claldpheef fvsgagdgdi
     3301 kiwgltvhsl lysfpaehpr ssffknigqn vfqgvtqlhv dsagrlfscg adgsmkvrql
     3361 perdcvvhtl y
//
LOCUS       XP_034195929            3367 aa            linear   INV 03-SEP-2025
DEFINITION  rabconnectin-3 alpha isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034195929
VERSION     XP_034195929.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340038.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195929.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3367
                     /product="rabconnectin-3 alpha isoform X5"
                     /calculated_mol_wt=375653
     Region          <192..>309
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          1371..2059
                     /region_name="Rav1p_C"
                     /note="RAVE protein 1 C terminal; pfam12234"
                     /db_xref="CDD:432413"
     Region          3090..3362
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(3094,3113,3117,3123..3124,3138..3139,3157,3161,
                     3167..3168,3184..3185,3202,3210,3216..3217,3233,3250,3255,
                     3261..3262,3274..3275,3293,3297,3303..3304,3316,3318,3348,
                     3354..3355)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          3144..3184
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3189..3231
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3238..3273
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3279..3311
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3367
                     /gene="Rbcn-3A"
                     /coded_by="XM_034340038.2:56..10159"
                     /db_xref="GeneID:117611773"
ORIGIN      
        1 mnchqilsga cnagdrcyav gsvegisfta yaagcnivil asnfervqii pgavhnyiri
       61 scldcstdtg kiaaayenqv cifeptplih stcshqleyr wvqtgslttd snitslswnl
      121 egtrlltgge llqlwhqniv pfqkehttke ntlrtqegvv tgreentsii lpesstvtfs
      181 iggdaespap sdtatgnepg awncvwkcrt atpvhlmsfs pdgtlfattg fndrlvkiwf
      241 enkqlftsrn idhtnasqsm gndsysfvyi ahpravthls wrktskhmpk gavsnmlvts
      301 crdnicrvwa etippevegl anmsqfegsd rhghhgkhrh hnmhkhrfmq rlkhmktcfh
      361 irrhakqqhq aghataptlp tlpstysvhd fhnnyqgtgq ypgmhfhlaa sinaetdipl
      421 vpslitgdpe repnfilhwl nnkemyftmq aesflqelar ivvekeeglh qqdaerpehd
      481 seeeglkkgv rlqpiqkpgg gtirstsqee hssdehhtth tvsshhslph svhsnlshqs
      541 lsnttsinsi atdvtssmnh apdsldtkie tllrdwhhnp dllfsihpid gsfliwhiew
      601 ldeyhpgsfr qaqisfstri pnafplgdas tmshsvslys hntggpllni revaksstks
      661 sneateiatp lpslieqdee qstltskagq ellknlenss dpnqnankdk ngqlengktn
      721 qetyndllth pspivsmvtk hsngtlnlwq ltfadktkfs qvlsighasr asghrfrvnd
      781 itchpvlpll vttshhnipe sgsqcrnsds setidsksdp nkmtkelspt gfcselilwr
      841 vdtvgplsks ggvselarin speisafsnv awiptllpst tlgnlsnsps acfvasdgec
      901 lrvyqavida rtllaevsss errsrmmdsm aslstdissd dgirhsihdk ikivsqqsta
      961 rpgcviqlda isdathdwqn tqflhvfqeq litgdrneek qagvdtsind lglmestlda
     1021 mvdlqqsaif eepfyivvle rtqqgttvhm wrlviasqpe ttglsgsmmy vpdshlvqde
     1081 eeegtpsryh qegrrsrrps qsrresqges dtffqrranq nshvlitttk vctqelplpd
     1141 gvevihaapa aghlssssiy pacfapyiiv tacsdstirf wkckvtkdpp ddklqyewce
     1201 wemirkdqes tiditgqpln vsaaysgria caykygksft rptksdpdsr yvnlcvaiye
     1261 cestggsewi ledtihlkni hlpripvdqh ldlsylydsr flqkkqrltq vlqtlshedv
     1321 rlprngenge stksnaglla vpsfstlqsl rksiiengnt cpltqkhlvq ldwvskedgs
     1381 hiltvavgsk imlftpvcsd laqanmkamk esqsnnrpil rkasslaqpq fvdeirwmkl
     1441 rkielttadg lpplpmqisw vrdgilvvgm dsemhvysqw kpnpkkecfh snlqhqesde
     1501 fqasrnlrde dlrtlanets qrrlanvssm phlsrvssin ltmldakkkr gmqsenisfd
     1561 ympdyglfea sriacpvlpq yhpkqlmell nsgkirwvka ilahlvrcig sscslradde
     1621 slmkqrggaw srsrtmsvsy vgttsplesr gsttqipeel mldyaeitsi splplwtlli
     1681 adketnlphq hktedkhdyn elfdsnldeg eslddmleed ydcsrqkdrr ssvperqgis
     1741 hfgprqgrll srllththlp glssldqmhl laladtvstc nvdfaerfai daaknaiake
     1801 nltgipdget istdslddcg lrfllamkhy nylirclpla qrahfqkqgv ssnnlvwafh
     1861 seteeellgl ipsyakgqpk wsvlkelgig wwirsntvlk rcvekiakaa yqqkqeplda
     1921 alyylamkkk nlvwglfrnk rddkmtsffa nnftevrwrk aaltnafall gkqrfehaaa
     1981 ffllagalrd aievcldkln diqlamviar lyeddttspn mrrllyeeil gydkdgqcqd
     2041 mnkahpdpfl rsmalwilkd yagslntlll tnvgtlhpqy ndesdkpegt tvsanpnvfn
     2101 fyvylrthpl lirqyiasta qdkkkghsvv isgfsygtdt ktqpdkqlml edsitplerq
     2161 lyfttahahf kagcpalale vlsklpskvm dtncedspsl lnspskartq dfqidtgiit
     2221 wnedhskqts ndtgaavldw sepvtkqted elvlnwedde veqgdsdspp lsmkldkket
     2281 ddnladaekd ekakaagqld imaqqlkfva clkilmeels tlatgfevdg gqlryqlyvw
     2341 lerevealrq lcsysvnadg dannapeyeg gmvddvqsyr pgeqptlhei lvadkldfea
     2401 kvqravkrkk wlkanetllr tllsycslhg asggglasvr melvlllqel qqektqqqll
     2461 splpfpttlp llsasvacnk tvvadpvrhl qslahdmlqt lvelrnppmp trnthycevf
     2521 imrdlavals aciyqslcds dtfvmkhhqp dsfpavaeie tfsgghlvas nrhhrrfstd
     2581 dgvcitttps kwpgvtnlra llarekdedt pklnvllcea fvatymslfv yamwscdshi
     2641 lyrlvgqhfd nstwsclfgg gvkkllrvas tssqasgsgt tekvtsgsid agsseiqtaa
     2701 gamwntmasl tkqrvklnmk llgpftgqqp nmkedkptyr eqfvspqmsm isyflmkpqi
     2761 dseyadeidy dssdsavsdl dsseeeedvf dtnskpktri kdntehsnpn syswcvmrla
     2821 iarilqqqlq dflnvagiem qelpvnspli hgtlavvaqw qetlreelds kgpplvnyip
     2881 gcapdptptp gkpaihkyrs llekgntpfn trlasaapan rlwcylvrqe svqdifirav
     2941 fgkrrclssi lennqntidn lnrgtaedkg sdsgttslpe pvriihkeqd sisafclnqv
     3001 sqglmalatp revqemnisl llelpswled ecefdiinln kqpepepvqp tsflviqtaa
     3061 drpllaqspq pnspqpqsgi asqsgrgasv ilkhkidgir risshpllpl yltgsqdgsv
     3121 slwewghqta vatprppgtf akvtrvrfsq hgnkfgvads dghlslfqva cregsarpff
     3181 nyqchskvta dfvflgacsl iataghgseg rnvalwdtll pqnksliqgf tchdqgassl
     3241 ilapqhqlli sggkkgdini fdvrqrqqrh rfqahesaik claldpheef fvsgagdgdi
     3301 kiwgltvhsl lysfpaehpr ssffknigqg vtqlhvdsag rlfscgadgs mkvrqlperd
     3361 cvvhtly
//
LOCUS       XP_034195931            3369 aa            linear   INV 03-SEP-2025
DEFINITION  rabconnectin-3 alpha isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195931
VERSION     XP_034195931.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340040.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195931.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3369
                     /product="rabconnectin-3 alpha isoform X6"
                     /calculated_mol_wt=375966
     Region          61..98
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <110..>282
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          113..153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          187..233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          249..282
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1344..2032
                     /region_name="Rav1p_C"
                     /note="RAVE protein 1 C terminal; pfam12234"
                     /db_xref="CDD:432413"
     Region          3099..3364
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(3115,3119,3125..3126,3140..3141,3159,3163,
                     3169..3170,3186..3187,3204,3212,3218..3219,3235,3252,3257,
                     3263..3264,3276..3277,3295,3299,3305..3306,3318,3320,3350,
                     3356..3357)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          3146..3186
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3191..3233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3240..3275
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3281..3313
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3369
                     /gene="Rbcn-3A"
                     /coded_by="XM_034340040.2:56..10165"
                     /db_xref="GeneID:117611773"
ORIGIN      
        1 mnchqilsga cnagdrcyav gsvegisfta yaagcnivil asnfervqii pgavhnyiri
       61 scldcstdtg kiaaayenqv cifeptplih stcshqleyr wvqtgslttd snitslswnl
      121 egtrlltgge llqlwhqniv pfqkehtstv tfsiggdaes papsdtatgn epgawncvwk
      181 crtatpvhlm sfspdgtlfa ttgfndrlvk iwfenkqlft srnidhtnas qsmgndsysf
      241 vyiahpravt hlswrktskh mpkgavsnml vtscrdnicr vwaetippev eglanmsqfe
      301 gsdrhghhgk hrhhnmhkhr fmqrlkhmkt cfhirrhakq qhqaghatap tlptlpstys
      361 vhdfhnnyqg tgqypgmhfh laasinaetd iplvpslitg dperepnfil hwlnnkemyf
      421 tmqaesflqe larivvekee glhqqdaerp ehdseeeglk kgvrlqpiqk pgggtirsts
      481 qeehssdehh tthtvsshhs lphsvhsnls hqslsnttsi nsiatdvtss mnhapdsldt
      541 kietllrdwh hnpdllfsih pidgsfliwh iewldeyhpg sfrqaqisfs tripnafplg
      601 dastmshsvs lyshntggpl lnirevakss tkssneatei atplpslieq deeqstltsk
      661 agqellknle nssdpnqnan kdkngqleng ktnqetyndl lthpspivsm vtkhsngtln
      721 lwqltfadkt kfsqvlsigh asrasghrfr vnditchpvl pllvttshhn ipesgsqcrn
      781 sdssetidsk sdpnkmtkel sptgfcseli lwrvdtvgpl sksggvsela rinspeisaf
      841 snvawiptll psttlgnlsn spsacfvasd geclrvyqav idartllaev ssserrsrmm
      901 dsmaslstdi ssddgirhsi hdkikivsqq starpgcviq ldaisdathd wqntqflhvf
      961 qeqlitgdrn eekqagvdts indlglmest ldamvdlqqs aifeepfyiv vlertqqgtt
     1021 vhmwrlvias qpettglsgs mmyvpdshlv qdeeeegtps ryhqegrrsr rpsqsrresq
     1081 gesdtffqrr anqnshvlit ttkvctqelp lpdgveviha apaaghlsss siypacfapy
     1141 iivtacsdst irfwkckvtk dppddklqye wcewemirkd qestiditgq plnvsaaysg
     1201 riacaykygk sftrptksdp dsryvnlcva iyecestggs ewiledtihl knihlpripv
     1261 dqhldlsyly dsrflqkkqr ltqvlqtlsh edvrlprnge ngestksnag llavpsfstl
     1321 qslrksiien gntcpltqkh lvqldwvske dgshiltvav gskimlftpv csdlaqanmk
     1381 amkesqsnnr pilrkassla qpqfvdeirw mklrkieltt adglpplpmq iswvrdgilv
     1441 vgmdsemhvy sqwkpnpkke cfhsnlqhqe sdefqasrnl rdedlrtlan etsqrrlanv
     1501 ssmphlsrvs sinltmldak kkrgmqseni sfdympdygl feasriacpv lpqyhpkqlm
     1561 ellnsgkirw vkailahlvr cigsscslra ddeslmkqrg gawsrsrtms vsyvgttspl
     1621 esrgsttqip eelmldyaei tsisplplwt lliadketnl phqhktedkh dynelfdsnl
     1681 degeslddml eedydcsrqk drrssvperq gishfgprqg rllsrlltht hlpglssldq
     1741 mhllaladtv stcnvdfaer faidaaknai akenltgipd getistdsld dcglrfllam
     1801 khynylircl plaqrahfqk qgvssnnlvw afhseteeel lglipsyakg qpkwsvlkel
     1861 gigwwirsnt vlkrcvekia kaayqqkqep ldaalyylam kkknlvwglf rnkrddkmts
     1921 ffannftevr wrkaaltnaf allgkqrfeh aaaffllaga lrdaievcld klndiqlamv
     1981 iarlyeddtt spnmrrllye eilgydkdgq cqdmnkahpd pflrsmalwi lkdyagslnt
     2041 llltnvgtlh pqyndesdkp egttvsanpn vfnfyvylrt hpllirqyia staqdkkkgh
     2101 svvisgfsyg tdtktqpdkq lmledsitpl erqlyfttah ahfkagcpal alevlsklps
     2161 kvmdtnceds psllnspska rtqdfqidtg iitwnedhsk qtsndtgaav ldwsepvtkq
     2221 tedelvlnwe ddeveqgdsd spplsmkldk ketddnlada ekdekakaag qldimaqqlk
     2281 fvaclkilme elstlatgfe vdggqlryql yvwlerevea lrqlcsysvn adgdannape
     2341 yeggmvddvq syrpgeqptl heilvadkld feakvqravk rkkwlkanet llrtllsycs
     2401 lhgasgggla svrmelvlll qelqqektqq qllsplpfpt tlpllsasva cnktvvadpv
     2461 rhlqslahdm lqtlvelrnp pmptrnthyc evfimrdlav alsaciyqsl cdsdtfvmkh
     2521 hqpdsfpava eietfsgghl vasnrhhrrf stddgvcitt tpskwpgvtn lrallarekd
     2581 edtpklnvll ceafvatyms lfvyamwscd shilyrlvgq hfdnstwscl fgggvkkllr
     2641 vastssqasg sgttekvtsg sidagsseiq taagamwntm asltkqrvkl nmkllgpftg
     2701 qqpnmkedkp tyreqfvspq msmisyflmk pqidseyade idydssdsav sdldsseeee
     2761 dvfdtnskpk trikdntehs npnsyswcvm rlaiarilqq qlqdflnvag iemqelpvns
     2821 plihgtlavv aqwqetlree ldskgpplvn yipgcapdpt ptpgkpaihk yrsllekgnt
     2881 pfntrlasaa panrlwcylv rqesvqdifi ravfgkrrcl ssilennqnt idnlnrgtae
     2941 dkgsdsgtts lpepvriihk eqdsisafcl nqvsqglmal atprevqemn islllelpsw
     3001 ledecefdii nlnkqpepep vqptsflviq taadrpllaq spqpnspqpq sgiasqsgrg
     3061 asvmkgmpaf pgshdlrfcq fvadrskhll qpilkhkidg irrisshpll plyltgsqdg
     3121 svslwewghq tavatprppg tfakvtrvrf sqhgnkfgva dsdghlslfq vacregsarp
     3181 ffnyqchskv tadfvflgac sliataghgs egrnvalwdt llpqnksliq gftchdqgas
     3241 slilapqhql lisggkkgdi nifdvrqrqq rhrfqahesa ikclaldphe effvsgagdg
     3301 dikiwgltvh sllysfpaeh prssffknig qgvtqlhvds agrlfscgad gsmkvrqlpe
     3361 rdcvvhtly
//
LOCUS       XP_034195930            3340 aa            linear   INV 03-SEP-2025
DEFINITION  rabconnectin-3 alpha isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034195930
VERSION     XP_034195930.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340039.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195930.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3340
                     /product="rabconnectin-3 alpha isoform X7"
                     /calculated_mol_wt=372669
     Region          61..98
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          <110..>282
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          113..153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          187..233
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          249..282
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          1344..2032
                     /region_name="Rav1p_C"
                     /note="RAVE protein 1 C terminal; pfam12234"
                     /db_xref="CDD:432413"
     Region          3063..3335
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(3067,3086,3090,3096..3097,3111..3112,3130,3134,
                     3140..3141,3157..3158,3175,3183,3189..3190,3206,3223,3228,
                     3234..3235,3247..3248,3266,3270,3276..3277,3289,3291,3321,
                     3327..3328)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          3117..3157
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3162..3204
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3211..3246
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3252..3284
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3340
                     /gene="Rbcn-3A"
                     /coded_by="XM_034340039.2:56..10078"
                     /db_xref="GeneID:117611773"
ORIGIN      
        1 mnchqilsga cnagdrcyav gsvegisfta yaagcnivil asnfervqii pgavhnyiri
       61 scldcstdtg kiaaayenqv cifeptplih stcshqleyr wvqtgslttd snitslswnl
      121 egtrlltgge llqlwhqniv pfqkehtstv tfsiggdaes papsdtatgn epgawncvwk
      181 crtatpvhlm sfspdgtlfa ttgfndrlvk iwfenkqlft srnidhtnas qsmgndsysf
      241 vyiahpravt hlswrktskh mpkgavsnml vtscrdnicr vwaetippev eglanmsqfe
      301 gsdrhghhgk hrhhnmhkhr fmqrlkhmkt cfhirrhakq qhqaghatap tlptlpstys
      361 vhdfhnnyqg tgqypgmhfh laasinaetd iplvpslitg dperepnfil hwlnnkemyf
      421 tmqaesflqe larivvekee glhqqdaerp ehdseeeglk kgvrlqpiqk pgggtirsts
      481 qeehssdehh tthtvsshhs lphsvhsnls hqslsnttsi nsiatdvtss mnhapdsldt
      541 kietllrdwh hnpdllfsih pidgsfliwh iewldeyhpg sfrqaqisfs tripnafplg
      601 dastmshsvs lyshntggpl lnirevakss tkssneatei atplpslieq deeqstltsk
      661 agqellknle nssdpnqnan kdkngqleng ktnqetyndl lthpspivsm vtkhsngtln
      721 lwqltfadkt kfsqvlsigh asrasghrfr vnditchpvl pllvttshhn ipesgsqcrn
      781 sdssetidsk sdpnkmtkel sptgfcseli lwrvdtvgpl sksggvsela rinspeisaf
      841 snvawiptll psttlgnlsn spsacfvasd geclrvyqav idartllaev ssserrsrmm
      901 dsmaslstdi ssddgirhsi hdkikivsqq starpgcviq ldaisdathd wqntqflhvf
      961 qeqlitgdrn eekqagvdts indlglmest ldamvdlqqs aifeepfyiv vlertqqgtt
     1021 vhmwrlvias qpettglsgs mmyvpdshlv qdeeeegtps ryhqegrrsr rpsqsrresq
     1081 gesdtffqrr anqnshvlit ttkvctqelp lpdgveviha apaaghlsss siypacfapy
     1141 iivtacsdst irfwkckvtk dppddklqye wcewemirkd qestiditgq plnvsaaysg
     1201 riacaykygk sftrptksdp dsryvnlcva iyecestggs ewiledtihl knihlpripv
     1261 dqhldlsyly dsrflqkkqr ltqvlqtlsh edvrlprnge ngestksnag llavpsfstl
     1321 qslrksiien gntcpltqkh lvqldwvske dgshiltvav gskimlftpv csdlaqanmk
     1381 amkesqsnnr pilrkassla qpqfvdeirw mklrkieltt adglpplpmq iswvrdgilv
     1441 vgmdsemhvy sqwkpnpkke cfhsnlqhqe sdefqasrnl rdedlrtlan etsqrrlanv
     1501 ssmphlsrvs sinltmldak kkrgmqseni sfdympdygl feasriacpv lpqyhpkqlm
     1561 ellnsgkirw vkailahlvr cigsscslra ddeslmkqrg gawsrsrtms vsyvgttspl
     1621 esrgsttqip eelmldyaei tsisplplwt lliadketnl phqhktedkh dynelfdsnl
     1681 degeslddml eedydcsrqk drrssvperq gishfgprqg rllsrlltht hlpglssldq
     1741 mhllaladtv stcnvdfaer faidaaknai akenltgipd getistdsld dcglrfllam
     1801 khynylircl plaqrahfqk qgvssnnlvw afhseteeel lglipsyakg qpkwsvlkel
     1861 gigwwirsnt vlkrcvekia kaayqqkqep ldaalyylam kkknlvwglf rnkrddkmts
     1921 ffannftevr wrkaaltnaf allgkqrfeh aaaffllaga lrdaievcld klndiqlamv
     1981 iarlyeddtt spnmrrllye eilgydkdgq cqdmnkahpd pflrsmalwi lkdyagslnt
     2041 llltnvgtlh pqyndesdkp egttvsanpn vfnfyvylrt hpllirqyia staqdkkkgh
     2101 svvisgfsyg tdtktqpdkq lmledsitpl erqlyfttah ahfkagcpal alevlsklps
     2161 kvmdtnceds psllnspska rtqdfqidtg iitwnedhsk qtsndtgaav ldwsepvtkq
     2221 tedelvlnwe ddeveqgdsd spplsmkldk ketddnlada ekdekakaag qldimaqqlk
     2281 fvaclkilme elstlatgfe vdggqlryql yvwlerevea lrqlcsysvn adgdannape
     2341 yeggmvddvq syrpgeqptl heilvadkld feakvqravk rkkwlkanet llrtllsycs
     2401 lhgasgggla svrmelvlll qelqqektqq qllsplpfpt tlpllsasva cnktvvadpv
     2461 rhlqslahdm lqtlvelrnp pmptrnthyc evfimrdlav alsaciyqsl cdsdtfvmkh
     2521 hqpdsfpava eietfsgghl vasnrhhrrf stddgvcitt tpskwpgvtn lrallarekd
     2581 edtpklnvll ceafvatyms lfvyamwscd shilyrlvgq hfdnstwscl fgggvkkllr
     2641 vastssqasg sgttekvtsg sidagsseiq taagamwntm asltkqrvkl nmkllgpftg
     2701 qqpnmkedkp tyreqfvspq msmisyflmk pqidseyade idydssdsav sdldsseeee
     2761 dvfdtnskpk trikdntehs npnsyswcvm rlaiarilqq qlqdflnvag iemqelpvns
     2821 plihgtlavv aqwqetlree ldskgpplvn yipgcapdpt ptpgkpaihk yrsllekgnt
     2881 pfntrlasaa panrlwcylv rqesvqdifi ravfgkrrcl ssilennqnt idnlnrgtae
     2941 dkgsdsgtts lpepvriihk eqdsisafcl nqvsqglmal atprevqemn islllelpsw
     3001 ledecefdii nlnkqpepep vqptsflviq taadrpllaq spqpnspqpq sgiasqsgrg
     3061 asvilkhkid girrisshpl lplyltgsqd gsvslwewgh qtavatprpp gtfakvtrvr
     3121 fsqhgnkfgv adsdghlslf qvacregsar pffnyqchsk vtadfvflga csliataghg
     3181 segrnvalwd tllpqnksli qgftchdqga sslilapqhq llisggkkgd inifdvrqrq
     3241 qrhrfqahes aikclaldph eeffvsgagd gdikiwgltv hsllysfpae hprssffkni
     3301 gqgvtqlhvd sagrlfscga dgsmkvrqlp erdcvvhtly
//
LOCUS       XP_034196373            1251 aa            linear   INV 03-SEP-2025
DEFINITION  protein girdin [Osmia lignaria lignaria].
ACCESSION   XP_034196373
VERSION     XP_034196373.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340482.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196373.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1251
                     /product="protein girdin"
                     /calculated_mol_wt=144616
     Region          10..153
                     /region_name="HkD_HkRP"
                     /note="Hook domain found in the Hook-related protein
                     (HkRP) family; cd22223"
                     /db_xref="CDD:411794"
     Site            order(121,127,130..131,133,138,141..142,144..145,148..149,
                     152)
                     /site_type="other"
                     /note="putative LIC-binding interface [polypeptide
                     binding]"
                     /db_xref="CDD:411794"
     Region          <211..945
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1251
                     /gene="Girdin"
                     /coded_by="XM_034340482.2:275..4030"
                     /db_xref="GeneID:117611955"
ORIGIN      
        1 masseiddll sgplvtwfts cledpnslts yydlvdgvll hnvflqidpe pihdeivppe
       61 gnslirtknl ravvdnmkqf yeeelghlvl rlpdtvnlgk epehyvaemk lllllllgca
      121 vqcpnkekfi tniktlnvdt qlaivdcikq vtdhqdivit qdamenvnmg clfgqikklt
      181 qeldlyrqkw rntvinenve gndssisgep eeinkiqqsn vviksenedn hhyaveladw
      241 ksrvrkqrqe leektealle ckeeleyhkm lvnklkqenq elmheartak syrdeldavi
      301 eradradrle levvryrekl tdiefyktri eelrednrvl metremledq lnssrrradk
      361 vleleseiik ykqllndmal eraadkekyq elveentqlq kltkaaanea aladsisdte
      421 epahtdnrls eqltnnaqtr alklelenrr lvtlidslke nsfhenssrv lelekekkkl
      481 slkieslndn ierltqqnsd lelvwkqald enkklhnslk nqkvasekqq qefqvqhvkv
      541 ieleknydta vkekqrvqtl lesvqrradd lernfevanq kveelkiven nikeinskcl
      601 dlesklvvte kekdvaqrdi hryretieek dvaldkaans ievlerkvmq leqdlhdsvt
      661 qisrlqeier sskeldsraa idretleilq snlvaeklnt qqlytvlekl gfsdntllsl
      721 pldnilkkia qipevvsyvq esndscccdk sipesktfes netgnlhstl eatveslkke
      781 qehlqmklat aqtasenlls ehaklqvqit tlqsqnnsla aqhtalqlan sqlvaekeel
      841 lkqrssqqqt haklvhdqvt lqslheqlnn eyenlfhehd tlksslrdvk neirmlresy
      901 egikeknkll qtekeslvnd akslnnlrge hsklkddfrn lytateklkm eyrnlqedyr
      961 knkietnrls lkltemqgel stkdercsnl elqvnklnqr cevllhmnsg ldndrrslmd
     1021 hisllfsqyh ellthsledk ehyhmeekmy tdtvnylyrq kekledkime hykklescts
     1081 kkkgfgsnlv rrvrkasefl nknrrswaed skhsegkiye sesggndsdg stedhrlags
     1141 arglgsdals lghpgtrrtv yytddsppps anlpeqgddr rsilleqtpr sevqveprpv
     1201 liynkvsavi nesknlnnsd mkdveqeeiv etpvdkdtka gnpvwyeygc v
//
LOCUS       XP_034195933             982 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611774 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195933
VERSION     XP_034195933.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340042.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195933.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..982
                     /product="uncharacterized protein LOC117611774 isoform X1"
                     /calculated_mol_wt=108517
     Region          577..626
                     /region_name="SWAP"
                     /note="Suppressor-of-White-APricot splicing regulator;
                     smart00648"
                     /db_xref="CDD:197818"
     Region          900..944
                     /region_name="G-patch"
                     /note="G-patch domain; pfam01585"
                     /db_xref="CDD:396249"
     CDS             1..982
                     /gene="LOC117611774"
                     /coded_by="XM_034340042.2:64..3012"
                     /db_xref="GeneID:117611774"
ORIGIN      
        1 mayrgvkgsd pfvsktsrne rfaqmskqeq iiqqkkleiq akmqeqkake avenmrktna
       61 llpnsagdqa snstkkeeek ptasqtinlf andgsfldqf kkiannskgg getesmfplk
      121 seekkeegsr enayekeqkr nsdrirdsen rrdrrrgdsr wgrnsdkrhs sssspsptrr
      181 stsqspearr sfnqsssngq rmqqsqqqfl ppngnssnvp sfapqsmihl tgvpppnmgn
      241 iitnvpppnl sslpppkvtg nsysgtnnqp gnprmqipss haihqpppsl svqsvppntn
      301 ipppnihmar tvppnvqnlp ppppppppls stpssstall plssspssss sftstcastc
      361 ssaspltsst mhlspltsss splttplsms slpstpsatl tstasgtqqr mfpnaqqrgv
      421 pppgplqptn lpppnipppn llstmtqmpp nmmpipgsqt itvnvpnatn vpnvpppnvp
      481 ppvmmpappp vhnvpsnins vpppnlnipp pnvppppppp sliqntappp lmstsyplpn
      541 qvsqvamgpp nlqvpppvrp miglrpseql vcpveaeqla rivadcgdei eqeareknaq
      601 dpklwflhqk qsaaylqyrg lvakyraers tkesknsgae pycpeealsd nddndhanig
      661 khsftqetek kreresednc tyrskednke erkrkrrsrw gdpdnkvpit eintvtarkq
      721 qsgsllqpgi aipgqigqpa viipsakmqr teaknpmltk isrndpaliq yarqtfgtld
      781 lteeqwkkae dhykinllyq nllkkreevr rleaqgkhky eydsdeeteg gtwehklrsa
      841 emeatqmwae eltaqaegkh higdflppde lkkfleqyna vkqgkepdls dykeyklked
      901 nigfqmlqkl gwsegqglgs egngridpin katnrfdsag lgserpdgvs rdddefdayr
      961 krmmlayrfr pnplnnprrp yy
//
LOCUS       XP_034195934             978 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611774 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195934
VERSION     XP_034195934.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340043.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195934.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..978
                     /product="uncharacterized protein LOC117611774 isoform X2"
                     /calculated_mol_wt=108118
     Region          573..622
                     /region_name="SWAP"
                     /note="Suppressor-of-White-APricot splicing regulator;
                     smart00648"
                     /db_xref="CDD:197818"
     Region          896..940
                     /region_name="G-patch"
                     /note="G-patch domain; pfam01585"
                     /db_xref="CDD:396249"
     CDS             1..978
                     /gene="LOC117611774"
                     /coded_by="XM_034340043.2:64..3000"
                     /db_xref="GeneID:117611774"
ORIGIN      
        1 mayrgvkgsd pfvsktsrne rfaqmskqeq iiqqkkleiq akmqeqkake avenmrktna
       61 llpnsagdqa snstkkeeek ptasqtinlf andgsfldqf kkiannskgg getesmfplk
      121 seekkeegsr enayekeqkr nsdrirdsen rrdrrrgdsr wgrnsdkrhs sssspsptrr
      181 stsqspearr sfnqsssngq rmqqsqqqfl ppngnssnvp sfapqsmihl tgvpppnmgn
      241 iitnvpppnl sslpppkvtg nsysgtnnqp gnprmqipss haihqpppsl svqsvppntn
      301 ipppnihmar tvppnvqnlp ppppppppls stpssstall plssspssss sftstcastc
      361 ssaspltsst mhlspltsss splttplsms slpstpsatl tstasgtqqr mfpnaqqrgv
      421 pppgplqptn lpppnipppn llstmtqmpp nmmpipgsqt itvnvpnatn vpnvpppnvp
      481 ppvmmpappp vhnvpsnins vpppnlnipp pnvppppppp sliqntappp lmstsyplpn
      541 qvsqvamgpp nlqvpppvrp miglrpseql eaeqlariva dcgdeieqea reknaqdpkl
      601 wflhqkqsaa ylqyrglvak yraerstkes knsgaepycp eealsdnddn dhanigkhsf
      661 tqetekkrer esednctyrs kednkeerkr krrsrwgdpd nkvpiteint vtarkqqsgs
      721 llqpgiaipg qigqpaviip sakmqrteak npmltkisrn dpaliqyarq tfgtldltee
      781 qwkkaedhyk inllyqnllk kreevrrlea qgkhkyeyds deeteggtwe hklrsaemea
      841 tqmwaeelta qaegkhhigd flppdelkkf leqynavkqg kepdlsdyke yklkednigf
      901 qmlqklgwse gqglgsegng ridpinkatn rfdsaglgse rpdgvsrddd efdayrkrmm
      961 layrfrpnpl nnprrpyy
//
LOCUS       XP_034196374             602 aa            linear   INV 03-SEP-2025
DEFINITION  NGFI-A-binding protein homolog isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196374
VERSION     XP_034196374.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340483.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="NGFI-A-binding protein homolog isoform X1"
                     /calculated_mol_wt=64794
     Region          <3..325
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          121..198
                     /region_name="NCD1"
                     /note="NAB conserved region 1 (NCD1); pfam04904"
                     /db_xref="CDD:461473"
     Region          330..452
                     /region_name="NCD2"
                     /note="NAB conserved region 2 (NCD2); pfam04905"
                     /db_xref="CDD:461474"
     CDS             1..602
                     /gene="nab"
                     /coded_by="XM_034340483.2:678..2486"
                     /db_xref="GeneID:117611956"
ORIGIN      
        1 mesrvlggpt teetavavtt tcnigtpspv pttpinavpg vggagagtlt sttctiqnpv
       61 vvvaspptkh qqsatvlsta pviptvptvp tavstvtavt atvpkilsrn vngtlvqtsv
      121 pqneaelqly rvmqrasllg yydtllemgg ddvqqlcdag eeefleimal vgmaskplhv
      181 rrlqkalqew ltnpslfqtp vvptnatckn pgsigfpcas prpqvqnlqg ftttsqtvta
      241 tpyvsshvsl tplpcrstsp ivsvtsvtap vasttfrqsp spntslpyat shspgvlqvg
      301 tcesscgsgt tpspsgggga pasptqptpt llqsqiqrla eaaerlaati rpvdpkphnv
      361 kkkicknlei vmampdsdpr rmeeirkyaa iygrfdckrk pekpltlhev svneaaaqic
      421 rfvpalltrr delfplarrv vrdsgyhysk shylsvsrsr engeeneper tkrqkleleg
      481 enedatqeel dlrcpeidin nstmrrhrss spvwrkknnn gmfggnieei sdsdsqlsfs
      541 neesssihdt neveadntdk esdfnlkvia srgdniiava npalmecnhp ikeeptdekp
      601 tl
//
LOCUS       XP_034196375             601 aa            linear   INV 03-SEP-2025
DEFINITION  NGFI-A-binding protein homolog isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196375
VERSION     XP_034196375.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340484.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..601
                     /product="NGFI-A-binding protein homolog isoform X2"
                     /calculated_mol_wt=64665
     Region          <3..325
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          121..198
                     /region_name="NCD1"
                     /note="NAB conserved region 1 (NCD1); pfam04904"
                     /db_xref="CDD:461473"
     Region          330..452
                     /region_name="NCD2"
                     /note="NAB conserved region 2 (NCD2); pfam04905"
                     /db_xref="CDD:461474"
     CDS             1..601
                     /gene="nab"
                     /coded_by="XM_034340484.2:678..2483"
                     /db_xref="GeneID:117611956"
ORIGIN      
        1 mesrvlggpt teetavavtt tcnigtpspv pttpinavpg vggagagtlt sttctiqnpv
       61 vvvaspptkh qqsatvlsta pviptvptvp tavstvtavt atvpkilsrn vngtlvqtsv
      121 pqneaelqly rvmqrasllg yydtllemgg ddvqqlcdag eeefleimal vgmaskplhv
      181 rrlqkalqew ltnpslfqtp vvptnatckn pgsigfpcas prpqvqnlqg ftttsqtvta
      241 tpyvsshvsl tplpcrstsp ivsvtsvtap vasttfrqsp spntslpyat shspgvlqvg
      301 tcesscgsgt tpspsgggga pasptqptpt llqsqiqrla eaaerlaati rpvdpkphnv
      361 kkkicknlei vmampdsdpr rmeeirkyaa iygrfdckrk pekpltlhev svneaaaqic
      421 rfvpalltrr delfplarrv vrdsgyhysk shylsvsrsr engeeneper tkrqkleleg
      481 enedatqeld lrcpeidinn stmrrhrsss pvwrkknnng mfggnieeis dsdsqlsfsn
      541 eesssihdtn eveadntdke sdfnlkvias rgdniiavan palmecnhpi keeptdekpt
      601 l
//
LOCUS       XP_034196376             570 aa            linear   INV 03-SEP-2025
DEFINITION  NGFI-A-binding protein homolog isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196376
VERSION     XP_034196376.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="NGFI-A-binding protein homolog isoform X3"
                     /calculated_mol_wt=60893
     Region          <3..325
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          121..198
                     /region_name="NCD1"
                     /note="NAB conserved region 1 (NCD1); pfam04904"
                     /db_xref="CDD:461473"
     Region          330..>420
                     /region_name="NCD2"
                     /note="NAB conserved region 2 (NCD2); pfam04905"
                     /db_xref="CDD:461474"
     CDS             1..570
                     /gene="nab"
                     /coded_by="XM_034340485.2:678..2390"
                     /db_xref="GeneID:117611956"
ORIGIN      
        1 mesrvlggpt teetavavtt tcnigtpspv pttpinavpg vggagagtlt sttctiqnpv
       61 vvvaspptkh qqsatvlsta pviptvptvp tavstvtavt atvpkilsrn vngtlvqtsv
      121 pqneaelqly rvmqrasllg yydtllemgg ddvqqlcdag eeefleimal vgmaskplhv
      181 rrlqkalqew ltnpslfqtp vvptnatckn pgsigfpcas prpqvqnlqg ftttsqtvta
      241 tpyvsshvsl tplpcrstsp ivsvtsvtap vasttfrqsp spntslpyat shspgvlqvg
      301 tcesscgsgt tpspsgggga pasptqptpt llqsqiqrla eaaerlaati rpvdpkphnv
      361 kkkicknlei vmampdsdpr rmeeirkyaa iygrfdckrk pekpltlhev svneaaaqic
      421 slsvsrsren geenepertk rqklelegen edatqeeldl rcpeidinns tmrrhrsssp
      481 vwrkknnngm fggnieeisd sdsqlsfsne esssihdtne veadntdkes dfnlkviasr
      541 gdniiavanp almecnhpik eeptdekptl
//
LOCUS       XP_076546660             511 aa            linear   INV 03-SEP-2025
DEFINITION  NGFI-A-binding protein homolog isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546660
VERSION     XP_076546660.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690545.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..511
                     /product="NGFI-A-binding protein homolog isoform X4"
                     /calculated_mol_wt=56248
     Region          30..107
                     /region_name="NCD1"
                     /note="NAB conserved region 1 (NCD1); pfam04904"
                     /db_xref="CDD:461473"
     Region          <83..>238
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          239..361
                     /region_name="NCD2"
                     /note="NAB conserved region 2 (NCD2); pfam04905"
                     /db_xref="CDD:461474"
     CDS             1..511
                     /gene="nab"
                     /coded_by="XM_076690545.1:4851..6386"
                     /db_xref="GeneID:117611956"
ORIGIN      
        1 mmtvvvvvvy verrseegnv nenvvqtsvp qneaelqlyr vmqrasllgy ydtllemggd
       61 dvqqlcdage eefleimalv gmaskplhvr rlqkalqewl tnpslfqtpv vptnatcknp
      121 gsigfpcasp rpqvqnlqgf tttsqtvtat pyvsshvslt plpcrstspi vsvtsvtapv
      181 asttfrqsps pntslpyats hspgvlqvgt cesscgsgtt pspsggggap asptqptptl
      241 lqsqiqrlae aaerlaatir pvdpkphnvk kkicknleiv mampdsdprr meeirkyaai
      301 ygrfdckrkp ekpltlhevs vneaaaqicr fvpalltrrd elfplarrvv rdsgyhysks
      361 hylsvsrsre ngeenepert krqklelege nedatqeeld lrcpeidinn stmrrhrsss
      421 pvwrkknnng mfggnieeis dsdsqlsfsn eesssihdtn eveadntdke sdfnlkvias
      481 rgdniiavan palmecnhpi keeptdekpt l
//
LOCUS       XP_034196380             581 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosaminyltransferase 7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196380
VERSION     XP_034196380.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196380.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="N-acetylgalactosaminyltransferase 7 isoform X1"
                     /calculated_mol_wt=67086
     Region          135..442
                     /region_name="pp-GalNAc-T"
                     /note="pp-GalNAc-T initiates the formation of mucin-type
                     O-linked glycans; cd02510"
                     /db_xref="CDD:133004"
     Site            order(139..141,143,172,202,205,209,225..227,311..312,
                     334..335,337,362,365,370)
                     /site_type="active"
                     /note="ligand binding site [active]"
                     /db_xref="CDD:133004"
     Site            order(225,227,362)
                     /site_type="active"
                     /note="Mn binding site [active]"
                     /db_xref="CDD:133004"
     Region          454..574
                     /region_name="beta-trefoil_Ricin_GALNT7"
                     /note="ricin B-type lectin domain, beta-trefoil fold,
                     found in polypeptide N-acetylgalactosaminyltransferase 7
                     (GALNT7) and similar proteins; cd23437"
                     /db_xref="CDD:467315"
     Site            order(471..474,482,484,487,492..493)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     Site            order(511..514,518,520,523,528..529)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     Site            order(550..553,559,561,564,569..570)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     CDS             1..581
                     /gene="Pgant7"
                     /coded_by="XM_034340489.2:207..1952"
                     /db_xref="GeneID:117611957"
ORIGIN      
        1 mrivqlkrnr lfsiivcsif vlftlyiiin fiaekpvsni ktywsaslks kqvpvlvkdl
       61 gnfeakhvsi rtgsgeggkp hilrddqqnd vqqseseygm nmvcsdeisl drsvpdtrmp
      121 eckhwnypet lpqtsviivf hnegwsvlmr tvhsvinrtp pqfleeillv ddysdkdnlk
      181 gdlesyieqw ngkvklirny ereglirtrs rgareakgev ivfldahcev nvnwlpplla
      241 piaadrtvmt vpvidgidhk tfeyrpvyqe grlyrgifew gmlykenelp areqktrpyn
      301 smpyksptha gglfainrey flslggydeg llvwggenfe lsfkiwqcgg silwvpcshv
      361 ghvyrgfmpy tfgklaqkkk gplitinykr vietwfddky keffytrepl aqlldhgdit
      421 eqlafkkrkr cksfqwymen vaydvfdkfp elppnihwge lrnvatgtcl dtmshsppsl
      481 matshchgfg nnqlirlnak gqlgvgerci sadslgikfv fcrlgtvdgp wqydektktl
      541 lhrvhkkcma lhpqtqqlsl mpcdinntyq qwsfhqihpr w
//
LOCUS       XP_034196381             581 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosaminyltransferase 7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196381
VERSION     XP_034196381.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340490.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196381.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..581
                     /product="N-acetylgalactosaminyltransferase 7 isoform X1"
                     /calculated_mol_wt=67086
     Region          135..442
                     /region_name="pp-GalNAc-T"
                     /note="pp-GalNAc-T initiates the formation of mucin-type
                     O-linked glycans; cd02510"
                     /db_xref="CDD:133004"
     Site            order(139..141,143,172,202,205,209,225..227,311..312,
                     334..335,337,362,365,370)
                     /site_type="active"
                     /note="ligand binding site [active]"
                     /db_xref="CDD:133004"
     Site            order(225,227,362)
                     /site_type="active"
                     /note="Mn binding site [active]"
                     /db_xref="CDD:133004"
     Region          454..574
                     /region_name="beta-trefoil_Ricin_GALNT7"
                     /note="ricin B-type lectin domain, beta-trefoil fold,
                     found in polypeptide N-acetylgalactosaminyltransferase 7
                     (GALNT7) and similar proteins; cd23437"
                     /db_xref="CDD:467315"
     Site            order(471..474,482,484,487,492..493)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     Site            order(511..514,518,520,523,528..529)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     Site            order(550..553,559,561,564,569..570)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     CDS             1..581
                     /gene="Pgant7"
                     /coded_by="XM_034340490.2:187..1932"
                     /db_xref="GeneID:117611957"
ORIGIN      
        1 mrivqlkrnr lfsiivcsif vlftlyiiin fiaekpvsni ktywsaslks kqvpvlvkdl
       61 gnfeakhvsi rtgsgeggkp hilrddqqnd vqqseseygm nmvcsdeisl drsvpdtrmp
      121 eckhwnypet lpqtsviivf hnegwsvlmr tvhsvinrtp pqfleeillv ddysdkdnlk
      181 gdlesyieqw ngkvklirny ereglirtrs rgareakgev ivfldahcev nvnwlpplla
      241 piaadrtvmt vpvidgidhk tfeyrpvyqe grlyrgifew gmlykenelp areqktrpyn
      301 smpyksptha gglfainrey flslggydeg llvwggenfe lsfkiwqcgg silwvpcshv
      361 ghvyrgfmpy tfgklaqkkk gplitinykr vietwfddky keffytrepl aqlldhgdit
      421 eqlafkkrkr cksfqwymen vaydvfdkfp elppnihwge lrnvatgtcl dtmshsppsl
      481 matshchgfg nnqlirlnak gqlgvgerci sadslgikfv fcrlgtvdgp wqydektktl
      541 lhrvhkkcma lhpqtqqlsl mpcdinntyq qwsfhqihpr w
//
LOCUS       XP_034196383             482 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylgalactosaminyltransferase 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196383
VERSION     XP_034196383.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340492.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196383.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..482
                     /product="N-acetylgalactosaminyltransferase 7 isoform X2"
                     /calculated_mol_wt=55851
     Region          36..343
                     /region_name="pp-GalNAc-T"
                     /note="pp-GalNAc-T initiates the formation of mucin-type
                     O-linked glycans; cd02510"
                     /db_xref="CDD:133004"
     Site            order(40..42,44,73,103,106,110,126..128,212..213,235..236,
                     238,263,266,271)
                     /site_type="active"
                     /note="ligand binding site [active]"
                     /db_xref="CDD:133004"
     Site            order(126,128,263)
                     /site_type="active"
                     /note="Mn binding site [active]"
                     /db_xref="CDD:133004"
     Region          355..475
                     /region_name="beta-trefoil_Ricin_GALNT7"
                     /note="ricin B-type lectin domain, beta-trefoil fold,
                     found in polypeptide N-acetylgalactosaminyltransferase 7
                     (GALNT7) and similar proteins; cd23437"
                     /db_xref="CDD:467315"
     Site            order(372..375,383,385,388,393..394)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     Site            order(412..415,419,421,424,429..430)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     Site            order(451..454,460,462,465,470..471)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467315"
     CDS             1..482
                     /gene="Pgant7"
                     /coded_by="XM_034340492.2:305..1753"
                     /db_xref="GeneID:117611957"
ORIGIN      
        1 mnmvcsdeis ldrsvpdtrm peckhwnype tlpqtsviiv fhnegwsvlm rtvhsvinrt
       61 ppqfleeill vddysdkdnl kgdlesyieq wngkvklirn yereglirtr srgareakge
      121 vivfldahce vnvnwlppll apiaadrtvm tvpvidgidh ktfeyrpvyq egrlyrgife
      181 wgmlykenel pareqktrpy nsmpykspth agglfainre yflslggyde gllvwggenf
      241 elsfkiwqcg gsilwvpcsh vghvyrgfmp ytfgklaqkk kgplitinyk rvietwfddk
      301 ykeffytrep laqlldhgdi teqlafkkrk rcksfqwyme nvaydvfdkf pelppnihwg
      361 elrnvatgtc ldtmshspps lmatshchgf gnnqlirlna kgqlgvgerc isadslgikf
      421 vfcrlgtvdg pwqydektkt llhrvhkkcm alhpqtqqls lmpcdinnty qqwsfhqihp
      481 rw
//
LOCUS       XP_034195937             556 aa            linear   INV 03-SEP-2025
DEFINITION  asparagine--tRNA ligase [Osmia lignaria lignaria].
ACCESSION   XP_034195937
VERSION     XP_034195937.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340046.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..556
                     /product="asparagine--tRNA ligase"
                     /calculated_mol_wt=64245
     Region          122..556
                     /region_name="AsnS"
                     /note="Aspartyl/asparaginyl-tRNA synthetase [Translation,
                     ribosomal structure and biogenesis]; COG0017"
                     /db_xref="CDD:439788"
     CDS             1..556
                     /gene="AsnRS"
                     /coded_by="XM_034340046.2:402..2072"
                     /db_xref="GeneID:117611776"
ORIGIN      
        1 mpekesshiv pdaiytsqkn gndetgngsd tnpfktvlra mhhagkepfp piyqdctkgn
       61 ekyelvsksq lkkvqkiwvr eqykseekqk klledeekrl knleeakalv iqedstlppa
      121 irikikdsis hrdqrvklyg wvhrlrrqgk almfitlrdg tgflqcvlnd tlcqtydali
      181 lsteasvevf gilkivpegk napnghellv dywtligasp pggadsilne ealpdvqldn
      241 rhimirgent srilimrsvl mqafrdhyke rnycevtppt lvqtqveggs tlfkldyfge
      301 eafltqssql yletcipamg dvyciaqsyr aeqsrtrrhl aeythveaec pfitfdelld
      361 rledlicdvv drvlksplgh lvkelnpdfk vpkkpfkrmn yseaieylrv nnitkedgtf
      421 yefgedipem perkmtdain epimlcrfpa eiksfymqrc pedksltesv dvllpnvgei
      481 vggsmriwdy dellegynre nieskpyywy tdqrkygtcp hggyglgler flcwllnryh
      541 irevclyprf lercrp
//
LOCUS       XP_034196384             503 aa            linear   INV 03-SEP-2025
DEFINITION  WASH complex subunit 1 [Osmia lignaria lignaria].
ACCESSION   XP_034196384
VERSION     XP_034196384.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340493.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196384.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..503
                     /product="WASH complex subunit 1"
                     /calculated_mol_wt=55203
     Region          7..290
                     /region_name="WASH_WAHD"
                     /note="WAHD domain of WASH complex; pfam11945"
                     /db_xref="CDD:463408"
     CDS             1..503
                     /gene="LOC117611958"
                     /coded_by="XM_034340493.2:231..1742"
                     /db_xref="GeneID:117611958"
ORIGIN      
        1 mperieigvi pdnlrheeti vqiaealdnl gsavdyvfes idkrlfengq rlsniknrat
       61 klqdrlqylq tnlnlkavkm ysaakypash tykeyemtik pecnntskip kaykcsvpik
      121 nvpetylssn cktedgqyva nnnlqeklqf yhvrsksnke aylngneasf plhmssinal
      181 lfnsmdnpyk vsikesshkt ndkqeiedap dsiiqpwksa etdssssyly tptlgevpqi
      241 nvppmlpdlp givddekfvl dlsfqspiap ssavttptvr pdlptptstt nkkdsstkfe
      301 hsvpnlpgfs endsakdsvq niappppppp plmpsdksis apsetqqplp sappmipppp
      361 pppppppppp ssktnvsdsq kdskknevie kkskianvdd rsnlmaeirn aggigraklr
      421 htvqsdekgn rwssasvggd lmadlhakla lrrkgiagst gsplermssl ippppkpnet
      481 ttsdrnsats eydsqpdtdd wde
//
LOCUS       XP_034196385             360 aa            linear   INV 03-SEP-2025
DEFINITION  adipokinetic hormone receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196385
VERSION     XP_034196385.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340494.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="adipokinetic hormone receptor isoform X1"
                     /calculated_mol_wt=41328
     Region          49..335
                     /region_name="7tmA_AKHR"
                     /note="adipokinetic hormone receptor, member of the class
                     A family of seven-transmembrane G protein-coupled
                     receptors; cd15382"
                     /db_xref="CDD:320504"
     Region          50..76
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320504"
     Region          84..111
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320504"
     Site            order(107,110..111,122..127,129..130,133,176,178..182,212,
                     215..217,219..221,223..224,284,287..288,290..291,294,
                     305..306,308..310,313,316..317)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320504"
     Region          122..152
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320504"
     Region          163..184
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320504"
     Region          212..241
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320504"
     Region          264..294
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320504"
     Region          306..331
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320504"
     CDS             1..360
                     /gene="AkhR"
                     /coded_by="XM_034340494.2:181..1263"
                     /db_xref="GeneID:117611959"
ORIGIN      
        1 mlvrvsgtvr simggdinva tstelpqleg nssdhlelpi dmrfneghiv sifiysvlmi
       61 isavgnttvl vliarrrrtt ksrihnmlmh laiadllvtf lmmpleigwa itvswkagda
      121 mcrimaffrv fglylssfil vcisidryya vihplqlwgv dkrgklmlcl awggsivcsm
      181 pqmivfhlet hpnitwysqc vtfntfptyt heityslfgm immywfplvv iiytytsill
      241 eicrrskkse gdkirrssmg fltrakvrtl kmtviivavf ficwtpyyvm slwywidrhs
      301 aykvdqriqk glflfactns cmnpivygaf nirdrhktsv rpttietrvt pltlslklld
//
LOCUS       XP_034196386             359 aa            linear   INV 03-SEP-2025
DEFINITION  adipokinetic hormone receptor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196386
VERSION     XP_034196386.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340495.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..359
                     /product="adipokinetic hormone receptor isoform X2"
                     /calculated_mol_wt=41271
     Region          49..334
                     /region_name="7tmA_AKHR"
                     /note="adipokinetic hormone receptor, member of the class
                     A family of seven-transmembrane G protein-coupled
                     receptors; cd15382"
                     /db_xref="CDD:320504"
     Region          50..76
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320504"
     Region          84..111
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320504"
     Site            order(107,110..111,122..127,129..130,133,176,178..182,212,
                     215..217,219..221,223..224,283,286..287,289..290,293,
                     304..305,307..309,312,315..316)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320504"
     Region          122..152
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320504"
     Region          163..184
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320504"
     Region          212..241
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320504"
     Region          263..293
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320504"
     Region          305..330
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320504"
     CDS             1..359
                     /gene="AkhR"
                     /coded_by="XM_034340495.2:186..1265"
                     /db_xref="GeneID:117611959"
ORIGIN      
        1 mlvrvsgtvr simggdinva tstelpqleg nssdhlelpi dmrfneghiv sifiysvlmi
       61 isavgnttvl vliarrrrtt ksrihnmlmh laiadllvtf lmmpleigwa itvswkagda
      121 mcrimaffrv fglylssfil vcisidryya vihplqlwgv dkrgklmlcl awggsivcsm
      181 pqmivfhlet hpnitwysqc vtfntfptyt heityslfgm immywfplvv iiytytsill
      241 eicrrskkse dkirrssmgf ltrakvrtlk mtviivavff icwtpyyvms lwywidrhsa
      301 ykvdqriqkg lflfactnsc mnpivygafn irdrhktsvr pttietrvtp ltlslklld
//
LOCUS       XP_034196388             289 aa            linear   INV 03-SEP-2025
DEFINITION  adipokinetic hormone receptor isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196388
VERSION     XP_034196388.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340497.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="adipokinetic hormone receptor isoform X3"
                     /calculated_mol_wt=32806
     Region          49..>267
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          51..75
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          85..108
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          123..145
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          166..182
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          213..236
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..289
                     /gene="AkhR"
                     /coded_by="XM_034340497.2:190..1059"
                     /db_xref="GeneID:117611959"
ORIGIN      
        1 mlvrvsgtvr simggdinva tstelpqleg nssdhlelpi dmrfneghiv sifiysvlmi
       61 isavgnttvl vliarrrrtt ksrihnmlmh laiadllvtf lmmpleigwa itvswkagda
      121 mcrimaffrv fglylssfil vcisidryya vihplqlwgv dkrgklmlcl awggsivcsm
      181 pqmivfhlet hpnitwysqc vtfntfptyt heityslfgm immywfplvv iiytytsill
      241 eicrrskkse gdkirrssmg fltraktsvr pttietrvtp ltlslklld
//
LOCUS       XP_034196389             288 aa            linear   INV 03-SEP-2025
DEFINITION  adipokinetic hormone receptor isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196389
VERSION     XP_034196389.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..288
                     /product="adipokinetic hormone receptor isoform X4"
                     /calculated_mol_wt=32749
     Region          49..>266
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          51..75
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          85..108
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          123..145
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          166..182
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          213..236
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..288
                     /gene="AkhR"
                     /coded_by="XM_034340498.2:193..1059"
                     /db_xref="GeneID:117611959"
ORIGIN      
        1 mlvrvsgtvr simggdinva tstelpqleg nssdhlelpi dmrfneghiv sifiysvlmi
       61 isavgnttvl vliarrrrtt ksrihnmlmh laiadllvtf lmmpleigwa itvswkagda
      121 mcrimaffrv fglylssfil vcisidryya vihplqlwgv dkrgklmlcl awggsivcsm
      181 pqmivfhlet hpnitwysqc vtfntfptyt heityslfgm immywfplvv iiytytsill
      241 eicrrskkse dkirrssmgf ltraktsvrp ttietrvtpl tlslklld
//
LOCUS       XP_034195941             275 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611780 [Osmia lignaria lignaria].
ACCESSION   XP_034195941
VERSION     XP_034195941.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340050.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195941.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="uncharacterized protein LOC117611780"
                     /calculated_mol_wt=32791
     CDS             1..275
                     /gene="LOC117611780"
                     /coded_by="XM_034340050.2:967..1794"
                     /db_xref="GeneID:117611780"
ORIGIN      
        1 mdefltvlae kyidvgvgki atenfyskgk iligkielfc qlvknspkry lieqsqktel
       61 ydltasidtl tlqvkqwtia lqeekldfsr fqknieadwk vmkltkknid enlkekltai
      121 iaeysskmls rnlehetrkk llqmdlklfe drlkrtsnvr ysrrnelyda clkskkvcfd
      181 qlkrydnnig vlhtckstll idadviekey fivqnqlipq ralynqlkke celnareall
      241 rkvehfrrnr aakiiqrywr fyyirvswkk rkgrk
//
LOCUS       XP_034195942             275 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611780 [Osmia lignaria lignaria].
ACCESSION   XP_034195942
VERSION     XP_034195942.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340051.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195942.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="uncharacterized protein LOC117611780"
                     /calculated_mol_wt=32791
     CDS             1..275
                     /gene="LOC117611780"
                     /coded_by="XM_034340051.2:967..1794"
                     /db_xref="GeneID:117611780"
ORIGIN      
        1 mdefltvlae kyidvgvgki atenfyskgk iligkielfc qlvknspkry lieqsqktel
       61 ydltasidtl tlqvkqwtia lqeekldfsr fqknieadwk vmkltkknid enlkekltai
      121 iaeysskmls rnlehetrkk llqmdlklfe drlkrtsnvr ysrrnelyda clkskkvcfd
      181 qlkrydnnig vlhtckstll idadviekey fivqnqlipq ralynqlkke celnareall
      241 rkvehfrrnr aakiiqrywr fyyirvswkk rkgrk
//
LOCUS       XP_034195943             275 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611780 [Osmia lignaria lignaria].
ACCESSION   XP_034195943
VERSION     XP_034195943.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340052.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195943.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="uncharacterized protein LOC117611780"
                     /calculated_mol_wt=32791
     CDS             1..275
                     /gene="LOC117611780"
                     /coded_by="XM_034340052.2:967..1794"
                     /db_xref="GeneID:117611780"
ORIGIN      
        1 mdefltvlae kyidvgvgki atenfyskgk iligkielfc qlvknspkry lieqsqktel
       61 ydltasidtl tlqvkqwtia lqeekldfsr fqknieadwk vmkltkknid enlkekltai
      121 iaeysskmls rnlehetrkk llqmdlklfe drlkrtsnvr ysrrnelyda clkskkvcfd
      181 qlkrydnnig vlhtckstll idadviekey fivqnqlipq ralynqlkke celnareall
      241 rkvehfrrnr aakiiqrywr fyyirvswkk rkgrk
//
LOCUS       XP_034194657             163 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610917 [Osmia lignaria lignaria].
ACCESSION   XP_034194657
VERSION     XP_034194657.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338766.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194657.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..163
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..163
                     /product="uncharacterized protein LOC117610917"
                     /calculated_mol_wt=18309
     CDS             1..163
                     /gene="LOC117610917"
                     /coded_by="XM_034338766.2:625..1116"
                     /db_xref="GeneID:117610917"
ORIGIN      
        1 msgtlplgef lrffsedhrq ehyesdfmsk dsnendkcne vlpkttfiae gvstatidgy
       61 nefpktfvae aatiasvnkf yknqptqtra tsniedysda mlaenrvctr nvhaittgge
      121 nkncqkpiss ketltrnmdp qvvlekiciq tqrrkslrkt tsi
//
LOCUS       XP_034195517             281 aa            linear   INV 03-SEP-2025
DEFINITION  arylalkylamine N-acetyltransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034195517
VERSION     XP_034195517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339626.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..281
                     /product="arylalkylamine N-acetyltransferase 1"
                     /calculated_mol_wt=32152
     Region          174..244
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..281
                     /gene="LOC117611617"
                     /coded_by="XM_034339626.2:685..1530"
                     /db_xref="GeneID:117611617"
ORIGIN      
        1 matyllksfg nsvakclkra sncngfgnir anfhstprnl qticsdrpay itrlalppdy
       61 dnvvdfmcdv yykdeptivn mglagteapl vwrkmmddlv rcglsfiaen hehciigaal
      121 nspttpqeiq mlcnlakccq tepiskvief yayvldsarv werfcvhtif eqsslaisee
      181 yrglgiaqrl iheswilard cgyrlfrmdc nnsycaricr gfgweevwri pfsqyvnnge
      241 vvfkcvkeph tdcqvfvdrl rycktfllpy kkckrvtppp a
//
LOCUS       XP_034195519             281 aa            linear   INV 03-SEP-2025
DEFINITION  arylalkylamine N-acetyltransferase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034195519
VERSION     XP_034195519.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339628.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..281
                     /product="arylalkylamine N-acetyltransferase 1"
                     /calculated_mol_wt=32152
     Region          174..244
                     /region_name="RimI"
                     /note="Ribosomal protein S18 acetylase RimI and related
                     acetyltransferases [Translation, ribosomal structure and
                     biogenesis]; COG0456"
                     /db_xref="CDD:440224"
     CDS             1..281
                     /gene="LOC117611617"
                     /coded_by="XM_034339628.2:685..1530"
                     /db_xref="GeneID:117611617"
ORIGIN      
        1 matyllksfg nsvakclkra sncngfgnir anfhstprnl qticsdrpay itrlalppdy
       61 dnvvdfmcdv yykdeptivn mglagteapl vwrkmmddlv rcglsfiaen hehciigaal
      121 nspttpqeiq mlcnlakccq tepiskvief yayvldsarv werfcvhtif eqsslaisee
      181 yrglgiaqrl iheswilard cgyrlfrmdc nnsycaricr gfgweevwri pfsqyvnnge
      241 vvfkcvkeph tdcqvfvdrl rycktfllpy kkckrvtppp a
//
LOCUS       XP_034195712             894 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate kinase 7 [Osmia lignaria lignaria].
ACCESSION   XP_034195712
VERSION     XP_034195712.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339821.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195712.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..894
                     /product="adenylate kinase 7"
                     /calculated_mol_wt=103254
     Region          <202..417
                     /region_name="WcaG"
                     /note="Nucleoside-diphosphate-sugar epimerase [Cell
                     wall/membrane/envelope biogenesis]; COG0451"
                     /db_xref="CDD:440220"
     Region          425..>641
                     /region_name="ADK"
                     /note="Adenylate kinase (ADK) catalyzes the reversible
                     phosphoryl transfer from adenosine triphosphates (ATP) to
                     adenosine monophosphates (AMP) and to yield adenosine
                     diphosphates (ADP). This enzyme is required for the
                     biosynthesis of ADP and is essential for...; cd01428"
                     /db_xref="CDD:238713"
     Site            order(454,459,525,551..552,554..555,559)
                     /site_type="other"
                     /note="AMP-binding site [chemical binding]"
                     /db_xref="CDD:238713"
     Region          754..794
                     /region_name="DD_AK7"
                     /note="dimerization/docking (D/D) domain found in
                     adenylate kinase 7 and similar proteins; cd22967"
                     /db_xref="CDD:438536"
     CDS             1..894
                     /gene="LOC117611701"
                     /coded_by="XM_034339821.2:130..2814"
                     /db_xref="GeneID:117611701"
ORIGIN      
        1 mttpkrasil vtksesdkki sldndrvife eskdgfaske lvepsfkpwr vfinhidsyh
       61 gtkiveflsd rvyvnpqsed lageeeeaee eetigdeeea ddeqsrveka ipedktskqy
      121 eiigttldpe ypkpedvtmi irgtknrdaf ladlincgiv vydithdsdq ideatwalqa
      181 iaqnlenmel iapkafkhsd evryfvlist imtwantkpl npeepelpft eddyrkrkph
      241 pnfkqhiqce kdvvtmkknt klkdklktlv icsgitygde egplhhlfkm awqnaeflpv
      301 fgegankipl lhvrdlaavv fdvlqrwpts ryiiaaeqep isqraivkki skalsngkvk
      361 kisneeafll peitqqifdv ttinlivepv yiadritwhf dipfrenina ivkayrtarn
      421 lrpmkiivlg ppaagktrva qylakhyeih ylhakplike tieqltkeie eitiaqarde
      481 aeeagagdvd dddepeddee apnveelqel ldeiernkqr nkdriddtll nrlfarklrs
      541 kqcqnqgyim dgypktleqa kglfggenln etieeeieee meidettgys impelvvsle
      601 asddflkeri iqrpqreihg thyteehmir rlkefrkrnt dentpvqffd eieihpliid
      661 veddvcpdmf ptvyrcieki gpprnyglta eqarearkrl eeelrgaeaa sksreekefm
      721 erkkereekm aewtnlmekl keeeeerlcv lglplrnylv kyvfptlsqg lievanlrpd
      781 dpidflaeyl fkenpegkmf epdftrtmts vldvieefqg evlppeqlsr ritelikqrr
      841 kqsaetlnst vsdvctskkt tvcvtpsyty cdtdtydgeg etmsdktedy dked
//
LOCUS       XP_076546661             106 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305747 [Osmia lignaria lignaria].
ACCESSION   XP_076546661
VERSION     XP_076546661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690546.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="uncharacterized protein LOC143305747"
                     /calculated_mol_wt=11962
     CDS             1..106
                     /gene="LOC143305747"
                     /coded_by="XM_076690546.1:6364..6684"
                     /db_xref="GeneID:143305747"
ORIGIN      
        1 maedkmdvdk emekkkegtq rkeetekkee vqkkedntkk eqvpktqeek lsaseikkmv
       61 kklmyggrrg grhggrggrw ggrrggrggr rgggrggdrr vifnif
//
LOCUS       XP_076546662             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305748 [Osmia lignaria lignaria].
ACCESSION   XP_076546662
VERSION     XP_076546662.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690547.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC143305748"
                     /calculated_mol_wt=56770
     Region          100..>195
                     /region_name="Gypsy"
                     /note="Gypsy protein; pfam07253"
                     /db_xref="CDD:254125"
     CDS             1..493
                     /gene="LOC143305748"
                     /coded_by="XM_076690547.1:453..1934"
                     /db_xref="GeneID:143305748"
ORIGIN      
        1 mkiiltltis iilvksiqtd pnathynyqq gigrimenyh ylhfyinits lrhtynkili
       61 sytilsgree lrnsslfrqt eslcneiyqn lqqiepltkr vkrglinglg tvikyifgnp
      121 dandldkinn yltslerqqh egiltlgktv siintisnti ngnteminkn lqnlantlse
      181 qstrldlfea iitlitqeqh flnllekikr sfvfsgevfd leiltyeqms dikahllkly
      241 srkelmlhyh nlldfrftqg sivtapdfii ytlkipilnp iefslfqrla tishnnqtei
      301 ittpwkltgt tvklfahkcq liydhdylcy rivaeeirpi yistniplvl vyplsnnstl
      361 iscnypitie ynrnkttlqg thfvqgndvq vqghnlnenl gelkqpeilp liirnyqlnl
      421 eplktltspt idiptfqklg iefhmsvtsv aiifliiigl lihftyrkkg kirqptqtin
      481 nedvielrqg gvm
//
LOCUS       XP_034194682             193 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610936 [Osmia lignaria lignaria].
ACCESSION   XP_034194682
VERSION     XP_034194682.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338791.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194682.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..193
                     /product="uncharacterized protein LOC117610936"
                     /calculated_mol_wt=21538
     Region          54..191
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..193
                     /gene="LOC117610936"
                     /coded_by="XM_034338791.2:2156..2737"
                     /db_xref="GeneID:117610936"
ORIGIN      
        1 macdsprlte gclssaqssa pisfddcttk llgiqwmpqt dtfgfsstit dqpirakmll
       61 qelwlhkinw ddplpsqiis rwfiiredln slaklsiprw fntwsnspve irgfsdayql
      121 amaaviyitv rlpsnnstts ivcsktkvap lkrltiprle lsaalllakh akyvestlkv
      181 tinathlwsd sqv
//
LOCUS       XP_034195612            1179 aa            linear   INV 03-SEP-2025
DEFINITION  neurogenic protein mastermind isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195612
VERSION     XP_034195612.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339721.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195612.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1179
                     /product="neurogenic protein mastermind isoform X1"
                     /calculated_mol_wt=122617
     Region          77..134
                     /region_name="MamL-1"
                     /note="MamL-1 domain; pfam09596"
                     /db_xref="CDD:462834"
     Region          605..>1008
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <625..744
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..1179
                     /gene="mam"
                     /coded_by="XM_034339721.2:1190..4729"
                     /db_xref="GeneID:117611672"
ORIGIN      
        1 meaggllprq ppqgppgnap gpisvcvgqa pnnnnannvn nvigqsggih dlnvtsqqlq
       61 lqqqmgqapg mgevltpkrq avvdrlrrri esyrrrqtdc iprfdqsfng lceqniqdtl
      121 vlkqrylesk akrqakktdk kqsdpvglss vhvvsvlrnl ldpikadsrq qkfvkrtaee
      181 lepagggsgd ggfgeppvkl qctqaqhqhq qgpggggggg phhghgqhpa gngtnstgqt
      241 hggggggggg gggggneglt kfsveivqql efttsaansq aqqistnvtv kaltnasvks
      301 dllnassspk sgpdtpasgt sraqagngng ggpgsggagt ggpaapggig cvdignlvec
      361 kqepdnefvd leqcaaalek daaangagfp gfsdfmgddt gdeiitsdaf kdliseisdf
      421 hpefmkdfdf eekipvdala nnaavaaaaa aaaaannnnn nsiatnngqi kieddkdaih
      481 qqhgttnnnn vnngtgpnng nsmpansspg tlgsaqyspv rlpysgldfk semspaaqtl
      541 kqmaeqhqhk sqqlglggfn psaaaaaaar gptarspyae fpqfggasdy lgspgstgpa
      601 ggqsqtsagt gsyhknngts gfqsqqtndm fvgsqtqfaa gladmkrpqa gsggkpsmlg
      661 psggykqqys pygspgsmpn hgspgyplpp rgsqgggpnq tgsqgpftss tpprppsgpg
      721 tstlqinqaq qlhinnpghq iqvsagqhmq ftgdlkpgvs vaaqqgmyfn qqqsqnqsgp
      781 ggqndtycsv sqsqtinftq qslkqraaaa ggvpqpagap garnhpqqvp qnqvdqhqha
      841 kllqqqqlmr aqqvmqqqqh maggmggvrp pppeykaaqa qmmhagigmg qqarfpntgp
      901 mrrvtqqpmp psgpmmrpqm aqqqqqalha aggnmymggg smaaiggmhq mhqrlgyprt
      961 nnqrppnvsv gppeslgnsi agrgaqqewr hvlmqqqgfq aqmrsqfnqq ghqggfgmgg
     1021 tggmqmnaaq mqhqqlirsq sggiggsgma nstqmqqlls qqhqqqtlam qqsnnqmslq
     1081 mqmsqsssvn svsstgtgsp lhphqqygag spgvrslpqq qqqqqhsqpq atttdpsvaa
     1141 adfsleflen lptgdtsnfs aqellnslds tagfnldil
//
LOCUS       XP_034195613            1178 aa            linear   INV 03-SEP-2025
DEFINITION  neurogenic protein mastermind isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195613
VERSION     XP_034195613.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339722.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195613.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1178
                     /product="neurogenic protein mastermind isoform X2"
                     /calculated_mol_wt=122489
     Region          77..134
                     /region_name="MamL-1"
                     /note="MamL-1 domain; pfam09596"
                     /db_xref="CDD:462834"
     Region          604..>1007
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <624..743
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..1178
                     /gene="mam"
                     /coded_by="XM_034339722.2:1190..4726"
                     /db_xref="GeneID:117611672"
ORIGIN      
        1 meaggllprq ppqgppgnap gpisvcvgqa pnnnnannvn nvigqsggih dlnvtsqqlq
       61 lqqqmgqapg mgevltpkrq avvdrlrrri esyrrrqtdc iprfdqsfng lceqniqdtl
      121 vlkqrylesk akrqakktdk kqsdpvglss vhvvsvlrnl ldpikadsrq kfvkrtaeel
      181 epagggsgdg gfgeppvklq ctqaqhqhqq gpgggggggp hhghgqhpag ngtnstgqth
      241 gggggggggg ggggnegltk fsveivqqle fttsaansqa qqistnvtvk altnasvksd
      301 llnassspks gpdtpasgts raqagngngg gpgsggagtg gpaapggigc vdignlveck
      361 qepdnefvdl eqcaaalekd aaangagfpg fsdfmgddtg deiitsdafk dliseisdfh
      421 pefmkdfdfe ekipvdalan naavaaaaaa aaaannnnnn siatnngqik ieddkdaihq
      481 qhgttnnnnv nngtgpnngn smpansspgt lgsaqyspvr lpysgldfks emspaaqtlk
      541 qmaeqhqhks qqlglggfnp saaaaaaarg ptarspyaef pqfggasdyl gspgstgpag
      601 gqsqtsagtg syhknngtsg fqsqqtndmf vgsqtqfaag ladmkrpqag sggkpsmlgp
      661 sggykqqysp ygspgsmpnh gspgyplppr gsqgggpnqt gsqgpftsst pprppsgpgt
      721 stlqinqaqq lhinnpghqi qvsagqhmqf tgdlkpgvsv aaqqgmyfnq qqsqnqsgpg
      781 gqndtycsvs qsqtinftqq slkqraaaag gvpqpagapg arnhpqqvpq nqvdqhqhak
      841 llqqqqlmra qqvmqqqqhm aggmggvrpp ppeykaaqaq mmhagigmgq qarfpntgpm
      901 rrvtqqpmpp sgpmmrpqma qqqqqalhaa ggnmymgggs maaiggmhqm hqrlgyprtn
      961 nqrppnvsvg ppeslgnsia grgaqqewrh vlmqqqgfqa qmrsqfnqqg hqggfgmggt
     1021 ggmqmnaaqm qhqqlirsqs ggiggsgman stqmqqllsq qhqqqtlamq qsnnqmslqm
     1081 qmsqsssvns vsstgtgspl hphqqygags pgvrslpqqq qqqqhsqpqa tttdpsvaaa
     1141 dfsleflenl ptgdtsnfsa qellnsldst agfnldil
//
LOCUS       XP_034195614            1163 aa            linear   INV 03-SEP-2025
DEFINITION  neurogenic protein mastermind isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195614
VERSION     XP_034195614.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339723.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195614.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1163
                     /product="neurogenic protein mastermind isoform X3"
                     /calculated_mol_wt=120839
     Region          77..134
                     /region_name="MamL-1"
                     /note="MamL-1 domain; pfam09596"
                     /db_xref="CDD:462834"
     Region          <609..728
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..1163
                     /gene="mam"
                     /coded_by="XM_034339723.2:1187..4678"
                     /db_xref="GeneID:117611672"
ORIGIN      
        1 meaggllprq ppqgppgnap gpisvcvgqa pnnnnannvn nvigqsggih dlnvtsqqlq
       61 lqqqmgqapg mgevltpkrq avvdrlrrri esyrrrqtdc iprfdqsfng lceqniqdtl
      121 vlkqrylesk akrqakktdk kqsdpvglss vhvqqkfvkr taeelepagg gsgdggfgep
      181 pvklqctqaq hqhqqgpggg ggggphhghg qhpagngtns tgqthggggg gggggggggn
      241 egltkfsvei vqqlefttsa ansqaqqist nvtvkaltna svksdllnas sspksgpdtp
      301 asgtsraqag ngngggpgsg gagtggpaap ggigcvdign lveckqepdn efvdleqcaa
      361 alekdaaang agfpgfsdfm gddtgdeiit sdafkdlise isdfhpefmk dfdfeekipv
      421 dalannaava aaaaaaaaan nnnnnsiatn ngqikieddk daihqqhgtt nnnnvnngtg
      481 pnngnsmpan sspgtlgsaq yspvrlpysg ldfksemspa aqtlkqmaeq hqhksqqlgl
      541 ggfnpsaaaa aaargptars pyaefpqfgg asdylgspgs tgpaggqsqt sagtgsyhkn
      601 ngtsgfqsqq tndmfvgsqt qfaagladmk rpqagsggkp smlgpsggyk qqyspygspg
      661 smpnhgspgy plpprgsqgg gpnqtgsqgp ftsstpprpp sgpgtstlqi nqaqqlhinn
      721 pghqiqvsag qhmqftgdlk pgvsvaaqqg myfnqqqsqn qsgpggqndt ycsvsqsqti
      781 nftqqslkqr aaaaggvpqp agapgarnhp qqvpqnqvdq hqhakllqqq qlmraqqvmq
      841 qqqhmaggmg gvrppppeyk aaqaqmmhag igmgqqarfp ntgpmrrvtq qpmppsgpmm
      901 rpqmaqqqqq alhaaggnmy mgggsmaaig gmhqmhqrlg yprtnnqrpp nvsvgppesl
      961 gnsiagrgaq qewrhvlmqq qgfqaqmrsq fnqqghqggf gmggtggmqm naaqmqhqql
     1021 irsqsggigg sgmanstqmq qllsqqhqqq tlamqqsnnq mslqmqmsqs ssvnsvsstg
     1081 tgsplhphqq ygagspgvrs lpqqqqqqqh sqpqatttdp svaaadfsle flenlptgdt
     1141 snfsaqelln sldstagfnl dil
//
LOCUS       XP_034195615            1162 aa            linear   INV 03-SEP-2025
DEFINITION  neurogenic protein mastermind isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195615
VERSION     XP_034195615.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339724.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195615.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1162
                     /product="neurogenic protein mastermind isoform X4"
                     /calculated_mol_wt=120711
     Region          77..134
                     /region_name="MamL-1"
                     /note="MamL-1 domain; pfam09596"
                     /db_xref="CDD:462834"
     Region          <608..727
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..1162
                     /gene="mam"
                     /coded_by="XM_034339724.2:1188..4676"
                     /db_xref="GeneID:117611672"
ORIGIN      
        1 meaggllprq ppqgppgnap gpisvcvgqa pnnnnannvn nvigqsggih dlnvtsqqlq
       61 lqqqmgqapg mgevltpkrq avvdrlrrri esyrrrqtdc iprfdqsfng lceqniqdtl
      121 vlkqrylesk akrqakktdk kqsdpvglss vhvqkfvkrt aeelepaggg sgdggfgepp
      181 vklqctqaqh qhqqgpgggg gggphhghgq hpagngtnst gqthgggggg ggggggggne
      241 gltkfsveiv qqlefttsaa nsqaqqistn vtvkaltnas vksdllnass spksgpdtpa
      301 sgtsraqagn gngggpgsgg agtggpaapg gigcvdignl veckqepdne fvdleqcaaa
      361 lekdaaanga gfpgfsdfmg ddtgdeiits dafkdlisei sdfhpefmkd fdfeekipvd
      421 alannaavaa aaaaaaaann nnnnsiatnn gqikieddkd aihqqhgttn nnnvnngtgp
      481 nngnsmpans spgtlgsaqy spvrlpysgl dfksemspaa qtlkqmaeqh qhksqqlglg
      541 gfnpsaaaaa aargptarsp yaefpqfgga sdylgspgst gpaggqsqts agtgsyhknn
      601 gtsgfqsqqt ndmfvgsqtq faagladmkr pqagsggkps mlgpsggykq qyspygspgs
      661 mpnhgspgyp lpprgsqggg pnqtgsqgpf tsstpprpps gpgtstlqin qaqqlhinnp
      721 ghqiqvsagq hmqftgdlkp gvsvaaqqgm yfnqqqsqnq sgpggqndty csvsqsqtin
      781 ftqqslkqra aaaggvpqpa gapgarnhpq qvpqnqvdqh qhakllqqqq lmraqqvmqq
      841 qqhmaggmgg vrppppeyka aqaqmmhagi gmgqqarfpn tgpmrrvtqq pmppsgpmmr
      901 pqmaqqqqqa lhaaggnmym gggsmaaigg mhqmhqrlgy prtnnqrppn vsvgppeslg
      961 nsiagrgaqq ewrhvlmqqq gfqaqmrsqf nqqghqggfg mggtggmqmn aaqmqhqqli
     1021 rsqsggiggs gmanstqmqq llsqqhqqqt lamqqsnnqm slqmqmsqss svnsvsstgt
     1081 gsplhphqqy gagspgvrsl pqqqqqqqhs qpqatttdps vaaadfslef lenlptgdts
     1141 nfsaqellns ldstagfnld il
//
LOCUS       XP_034195616            1012 aa            linear   INV 03-SEP-2025
DEFINITION  neurogenic protein mastermind isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034195616
VERSION     XP_034195616.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339725.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195616.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1012
                     /product="neurogenic protein mastermind isoform X5"
                     /calculated_mol_wt=104300
     Region          <458..577
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..1012
                     /gene="mam"
                     /coded_by="XM_034339725.2:4731..7769"
                     /db_xref="GeneID:117611672"
ORIGIN      
        1 mmqqkfvkrt aeelepaggg sgdggfgepp vklqctqaqh qhqqgpgggg gggphhghgq
       61 hpagngtnst gqthgggggg ggggggggne gltkfsveiv qqlefttsaa nsqaqqistn
      121 vtvkaltnas vksdllnass spksgpdtpa sgtsraqagn gngggpgsgg agtggpaapg
      181 gigcvdignl veckqepdne fvdleqcaaa lekdaaanga gfpgfsdfmg ddtgdeiits
      241 dafkdlisei sdfhpefmkd fdfeekipvd alannaavaa aaaaaaaann nnnnsiatnn
      301 gqikieddkd aihqqhgttn nnnvnngtgp nngnsmpans spgtlgsaqy spvrlpysgl
      361 dfksemspaa qtlkqmaeqh qhksqqlglg gfnpsaaaaa aargptarsp yaefpqfgga
      421 sdylgspgst gpaggqsqts agtgsyhknn gtsgfqsqqt ndmfvgsqtq faagladmkr
      481 pqagsggkps mlgpsggykq qyspygspgs mpnhgspgyp lpprgsqggg pnqtgsqgpf
      541 tsstpprpps gpgtstlqin qaqqlhinnp ghqiqvsagq hmqftgdlkp gvsvaaqqgm
      601 yfnqqqsqnq sgpggqndty csvsqsqtin ftqqslkqra aaaggvpqpa gapgarnhpq
      661 qvpqnqvdqh qhakllqqqq lmraqqvmqq qqhmaggmgg vrppppeyka aqaqmmhagi
      721 gmgqqarfpn tgpmrrvtqq pmppsgpmmr pqmaqqqqqa lhaaggnmym gggsmaaigg
      781 mhqmhqrlgy prtnnqrppn vsvgppeslg nsiagrgaqq ewrhvlmqqq gfqaqmrsqf
      841 nqqghqggfg mggtggmqmn aaqmqhqqli rsqsggiggs gmanstqmqq llsqqhqqqt
      901 lamqqsnnqm slqmqmsqss svnsvsstgt gsplhphqqy gagspgvrsl pqqqqqqqhs
      961 qpqatttdps vaaadfslef lenlptgdts nfsaqellns ldstagfnld il
//
LOCUS       XP_034195618            1011 aa            linear   INV 03-SEP-2025
DEFINITION  neurogenic protein mastermind isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195618
VERSION     XP_034195618.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339727.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195618.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1011
                     /product="neurogenic protein mastermind isoform X6"
                     /calculated_mol_wt=104172
     Region          <457..576
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     CDS             1..1011
                     /gene="mam"
                     /coded_by="XM_034339727.2:4731..7766"
                     /db_xref="GeneID:117611672"
ORIGIN      
        1 mmqkfvkrta eelepagggs gdggfgeppv klqctqaqhq hqqgpggggg ggphhghgqh
       61 pagngtnstg qthggggggg gggggggneg ltkfsveivq qlefttsaan sqaqqistnv
      121 tvkaltnasv ksdllnasss pksgpdtpas gtsraqagng ngggpgsgga gtggpaapgg
      181 igcvdignlv eckqepdnef vdleqcaaal ekdaaangag fpgfsdfmgd dtgdeiitsd
      241 afkdliseis dfhpefmkdf dfeekipvda lannaavaaa aaaaaaannn nnnsiatnng
      301 qikieddkda ihqqhgttnn nnvnngtgpn ngnsmpanss pgtlgsaqys pvrlpysgld
      361 fksemspaaq tlkqmaeqhq hksqqlglgg fnpsaaaaaa argptarspy aefpqfggas
      421 dylgspgstg paggqsqtsa gtgsyhknng tsgfqsqqtn dmfvgsqtqf aagladmkrp
      481 qagsggkpsm lgpsggykqq yspygspgsm pnhgspgypl pprgsqgggp nqtgsqgpft
      541 sstpprppsg pgtstlqinq aqqlhinnpg hqiqvsagqh mqftgdlkpg vsvaaqqgmy
      601 fnqqqsqnqs gpggqndtyc svsqsqtinf tqqslkqraa aaggvpqpag apgarnhpqq
      661 vpqnqvdqhq hakllqqqql mraqqvmqqq qhmaggmggv rppppeykaa qaqmmhagig
      721 mgqqarfpnt gpmrrvtqqp mppsgpmmrp qmaqqqqqal haaggnmymg ggsmaaiggm
      781 hqmhqrlgyp rtnnqrppnv svgppeslgn siagrgaqqe wrhvlmqqqg fqaqmrsqfn
      841 qqghqggfgm ggtggmqmna aqmqhqqlir sqsggiggsg manstqmqql lsqqhqqqtl
      901 amqqsnnqms lqmqmsqsss vnsvsstgtg splhphqqyg agspgvrslp qqqqqqqhsq
      961 pqatttdpsv aaadfslefl enlptgdtsn fsaqellnsl dstagfnldi l
//
LOCUS       XP_034195619             362 aa            linear   INV 03-SEP-2025
DEFINITION  elongator complex protein 4 [Osmia lignaria lignaria].
ACCESSION   XP_034195619
VERSION     XP_034195619.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339728.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..362
                     /product="elongator complex protein 4"
                     /calculated_mol_wt=41201
     Region          10..362
                     /region_name="PAXNEB"
                     /note="PAXNEB protein; pfam05625"
                     /db_xref="CDD:461696"
     CDS             1..362
                     /gene="Elp4"
                     /coded_by="XM_034339728.2:89..1177"
                     /db_xref="GeneID:117611674"
ORIGIN      
        1 mdrsgakhsr ipfipgtkps iknsqlliss gipsldhiig gglpvgslfl ieedrygtya
       61 kvmlryfmae givtnqplli askdvkpsdf vsdiptvite tpvekpfvtd eqmkiawryq
      121 nmklidtspt ggqvfghfyd ltktmkkevl eqanithwcd dsfpkkddmf enmtysrllk
      181 ciqeilkegq ylvseipekr qvlritihsl gsrlwfsdse ndshqdllkf lyhfrallry
      241 syavgvitvp vdcldnsnai veriehlsdi aiklesfags qketnplfkd yhgllhlkkm
      301 paintiaphk tescdltfkl rrkkffievl hlppelgdtv qreqdeepfg ccsqsrespl
      361 df
//
LOCUS       XP_034195620             362 aa            linear   INV 03-SEP-2025
DEFINITION  elongator complex protein 4 [Osmia lignaria lignaria].
ACCESSION   XP_034195620
VERSION     XP_034195620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..362
                     /product="elongator complex protein 4"
                     /calculated_mol_wt=41201
     Region          10..362
                     /region_name="PAXNEB"
                     /note="PAXNEB protein; pfam05625"
                     /db_xref="CDD:461696"
     CDS             1..362
                     /gene="Elp4"
                     /coded_by="XM_034339729.2:153..1241"
                     /db_xref="GeneID:117611674"
ORIGIN      
        1 mdrsgakhsr ipfipgtkps iknsqlliss gipsldhiig gglpvgslfl ieedrygtya
       61 kvmlryfmae givtnqplli askdvkpsdf vsdiptvite tpvekpfvtd eqmkiawryq
      121 nmklidtspt ggqvfghfyd ltktmkkevl eqanithwcd dsfpkkddmf enmtysrllk
      181 ciqeilkegq ylvseipekr qvlritihsl gsrlwfsdse ndshqdllkf lyhfrallry
      241 syavgvitvp vdcldnsnai veriehlsdi aiklesfags qketnplfkd yhgllhlkkm
      301 paintiaphk tescdltfkl rrkkffievl hlppelgdtv qreqdeepfg ccsqsrespl
      361 df
//
LOCUS       XP_034196330             557 aa            linear   INV 03-SEP-2025
DEFINITION  maltase 1-like [Osmia lignaria lignaria].
ACCESSION   XP_034196330
VERSION     XP_034196330.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340439.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196330.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..557
                     /product="maltase 1-like"
                     /calculated_mol_wt=64082
     Region          25..472
                     /region_name="AmyAc_maltase"
                     /note="Alpha amylase catalytic domain found in maltase
                     (also known as alpha glucosidase) and related proteins;
                     cd11328"
                     /db_xref="CDD:200467"
     Site            order(83,86,123,126,171,190,194,226,294,355..356)
                     /site_type="active"
                     /db_xref="CDD:200467"
     Site            order(226,294,356)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:200467"
     CDS             1..557
                     /gene="LOC117611930"
                     /coded_by="XM_034340439.2:455..2128"
                     /db_xref="GeneID:117611930"
ORIGIN      
        1 mktttnlvgi vvfltvafas aeiknkgwwk ntvfyqvypr sfmdsngdgv gdlkgitskl
       61 dyledigina vwlspiyksp nvdfgydisd fkavdpifgt sadlkelamk arkknlklvl
      121 dlvpnhtsde hkwfqlsinr tgkyadyyvw hdgivnetge rqppdnwksi tggvawtwnd
      181 qrkqfyfhqn fkqqpdlnys nphvqqemke vlkywldqgv dgfridsvyf mfesnitqne
      241 plinetydpt lyysynhilt kdqpetykll qswrdyvdey srqrnetekv ilteaysswe
      301 ntmkyyrygs dipfnfffit qtnanstasd frniinkwmq mmpkrqvanw vmgnhdqkrl
      361 strypgrgdq mimlemilpg vavtyngeei gmvdytglei fdsrdssrtp fqwddsmnag
      421 fsnasktwlp vnknyktlnl kkqktdavsh yklykalitl rksnvlqhgk lttavlnndv
      481 lavvrsikke avtllinfss kksvsvdlrs mlpytsatvi lsnvgskvqs ntkfasskvt
      541 lppkvsmvlh sgsrkan
//
LOCUS       XP_076546663             104 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611624 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546663
VERSION     XP_076546663.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690548.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..104
                     /product="uncharacterized protein LOC117611624 isoform X1"
                     /calculated_mol_wt=11395
     CDS             1..104
                     /gene="LOC117611624"
                     /coded_by="XM_076690548.1:677..991"
                     /db_xref="GeneID:117611624"
ORIGIN      
        1 mwvgiglval vslmkheypl gchryllygd gslcmnrqdk kdpsvaclrd gcmldaplsn
       61 sssglgllqt fllyievfkl egwrkaagcn seeaegqkvi ckrk
//
LOCUS       XP_076546664             100 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611624 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546664
VERSION     XP_076546664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690549.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..100
                     /product="uncharacterized protein LOC117611624 isoform X2"
                     /calculated_mol_wt=10880
     CDS             1..100
                     /gene="LOC117611624"
                     /coded_by="XM_076690549.1:677..979"
                     /db_xref="GeneID:117611624"
ORIGIN      
        1 mwvgiglval vslmkheypl gchryllygd gslcmnrqdk kdpsvaclrd gcmldaplsn
       61 sssglgllqt fllyievfkl egwrkaagcn seeaegqkvi
//
LOCUS       XP_034195516             296 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611616 [Osmia lignaria lignaria].
ACCESSION   XP_034195516
VERSION     XP_034195516.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339625.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195516.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..296
                     /product="uncharacterized protein LOC117611616"
                     /calculated_mol_wt=33344
     Region          139..284
                     /region_name="DUF4779"
                     /note="Domain of unknown function (DUF4779); pfam16009"
                     /db_xref="CDD:464971"
     CDS             1..296
                     /gene="LOC117611616"
                     /coded_by="XM_034339625.2:154..1044"
                     /db_xref="GeneID:117611616"
ORIGIN      
        1 mnvlgigwyt lyaficvcvq linargisvy vinprihdlr aiemepiygq iakypphfmg
       61 kklipikepr psrnlalple pdaemlpahy ervkppgvdh melkyaetqi vkdipkiqps
      121 kapaqtrraq qssekqrsea kndknesqkt vsveiketgd dltekkkvey vdaagrkkvh
      181 ddhdrvskhh teksksaddv shaaksndkv drvatgyrni yhkdeykknh dfydnddhgg
      241 hskqhgryne rhvstegtfk kgdshggsfd qgelqkekga knsgangktk ghdgfv
//
LOCUS       XP_034194190             217 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610664 [Osmia lignaria lignaria].
ACCESSION   XP_034194190
VERSION     XP_034194190.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338299.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194190.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="uncharacterized protein LOC117610664"
                     /calculated_mol_wt=24354
     CDS             1..217
                     /gene="LOC117610664"
                     /coded_by="XM_034338299.2:471..1124"
                     /db_xref="GeneID:117610664"
ORIGIN      
        1 mqegypedkk kniletyprk ddfyteapkv nieigpvmte valkrdehfa etqncigsai
       61 salgaavsmi leepedgidq ealmkylcdt gklltdvfhq qskarrafit plmnksvkpt
      121 vdatkadewl yakkfaeqvk eakdiekacs sikatpktin pksrdqgnsk yppakykqvg
      181 nqqkksvirf ksrpqrtsys tikasswaas hrlpprk
//
LOCUS       XP_076546665             295 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305749 [Osmia lignaria lignaria].
ACCESSION   XP_076546665
VERSION     XP_076546665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690550.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 7% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="uncharacterized protein LOC143305749"
                     /calculated_mol_wt=33677
     Region          75..231
                     /region_name="DDE_3"
                     /note="DDE superfamily endonuclease; cl46863"
                     /db_xref="CDD:481203"
     Region          231..290
                     /region_name="Transp_Tc5_C"
                     /note="Tc5 transposase C-terminal domain; pfam04236"
                     /db_xref="CDD:398081"
     CDS             1..295
                     /gene="LOC143305749"
                     /coded_by="XM_076690550.1:1..888"
                     /db_xref="GeneID:143305749"
ORIGIN      
        1 mqscspsnlf igryrivkvc slrrqsgfnl elhsgrtlal kgtkkveavi qsipstthsy
       61 tiqptisasg kllsplfvvl ketsgefgpr vqqtmfkptn ilatastsgk ltnehfkiwl
      121 kevyfpntnd ktallldsws ghcgrsivea tpenkelvvf tipagttkyl qpldvygfrv
      181 wknfvrrfsd tvillhyein lhtrdnilkl eslahnqfss prfenfikys wyksgyvssr
      241 pphfenpvef cyaadneprc hvcgdvaiit rawcrkplcl khffddyhfc heyvp
//
LOCUS       XP_076546666             100 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305750 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546666
VERSION     XP_076546666.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690551.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 2% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..100
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305750"
                     /calculated_mol_wt=11118
     CDS             1..100
                     /gene="LOC143305750"
                     /coded_by="XM_076690551.1:1..303"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:143305750"
ORIGIN      
        1 mshggyngtc cppwkplrpe wlwldhsret egswhvkpgl pharsqdksi ttehqrncpm
       61 twkkggkstq pleswvcpis casscrllqm qkststkgas
//
LOCUS       XP_076546667            1527 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611765 [Osmia lignaria lignaria].
ACCESSION   XP_076546667
VERSION     XP_076546667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690552.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 18% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1527
                     /product="uncharacterized protein LOC117611765"
                     /calculated_mol_wt=167920
     Region          <667..1238
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <819..1076
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..1527
                     /gene="LOC117611765"
                     /coded_by="XM_076690552.1:1..4584"
                     /db_xref="GeneID:117611765"
ORIGIN      
        1 mvngvhrspf stssaaslkw knrskgvkng clrvtnpntl nqeakfssdt stmtyecrnd
       61 rknfimsdfe esgklerass syatiierys nelefddwns splpkrnapm dlndfpalmt
      121 ntkhrpmklr sdrneritsg eskkteekgq tdwmskmedv ikdlkkitsk nemvdsksse
      181 kwalqsvneh aewmdrvvga hkkgklrkgd sdepkssttm qesvvdaane nrgsksnpit
      241 syinrgsigs stssssqgpt gtatgstkqp qielrmtpsq nesvvsinvd elaktagtsa
      301 keqlsvvisn nakrdtsasn vrsnfdsmqi sisentvpvk qlefsingkp vselksivar
      361 ankldvfssm dkveiripqt entfskqqtn tmrtgtnpsq vlnlqiaakf neprsqqpkd
      421 tgiktsttat pssvpssssn ppkptptptq vppyklprmq nfasktndea akitkspedv
      481 attkkindsp ledsrekigt lsavesdega vqranakvas nasqpssmnd nsrpvisndd
      541 rtssniipww ssedsfrkik kkgdtsknvs afdeklmkki egdtgaerkf dqtykpggqf
      601 idlvnvpkle tpakdltpkn llskekknss dagnfqktep pvkdsatkpv pkenedsfns
      661 snenfravrv rslrqsarik rssgarasnq mnrvkkvryr vkrprttkvv nkgiekpivs
      721 avrspkiiky vpvnakrisg neksetkaia agpkneeask agtkvekvae dasttgktnv
      781 qdsntvfktk dlkkpanesa disatssaak eqgerdesrr dlpsrresie depdqrikqi
      841 lkslkpiekr kdgtlidygv vlpsrksstk fgqvsksrsi skfdmkkeps nqekivepqk
      901 vtstkkkmlt epkseaqvki vepekissvr kpneitdfek kytknelsts kgkketseek
      961 svisvkknnl ikhdtpksme skknsdnqse kvkttqiegt sfkandkpkn vssedrinss
     1021 kvtaprdegs qsplrpknke kplegtfsvq sskastkssd tnvtnvsksq sadksknits
     1081 pekqdskitk sesqekakli gsysvppskv sikvsdinan vsksqspdks knittspekq
     1141 dskitksesq ekaklkgsys vppskvsikv sdtnakvsks qlpdksknit tspekqdski
     1201 tksesqekak lkgsysvpps kisikdskia kpesqekrnl gslpvqpski sikpsntnit
     1261 nvsksqspdk pknittssen qdskitkpef qekknlgplp vqpskisikp sntnitnvsk
     1321 shsldkskni akpvekqnsk stksefleki ksgplpvqpf kdsikasdtn itnfsklkpl
     1381 nepmsrarsa eqteskrtlk svvpekvple gtipssrgld rtaaridplp lmgtivspsp
     1441 qkssdkgtkn dgkseesnvr sekkvidtim aplegtinne stkkldnlms ldstrkvkve
     1501 avldpmalqi llqdllvsdl qgcllch
//
LOCUS       XP_076546668            1209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305751 [Osmia lignaria lignaria].
ACCESSION   XP_076546668
VERSION     XP_076546668.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690553.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1209
                     /product="uncharacterized protein LOC143305751"
                     /calculated_mol_wt=137688
     Region          <3..103
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          230..443
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(294..299,332..333,368,370..371,434..435)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(294..299,332,370)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            333
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          459..619
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <727..788
                     /region_name="metallo-dependent_hydrolases"
                     /note="Superfamily of metallo-dependent hydrolases (also
                     called amidohydrolase superfamily) is a large group of
                     proteins that show conservation in their 3-dimensional
                     fold (TIM barrel) and in details of their active site. The
                     vast majority of the members have a...; cl00281"
                     /db_xref="CDD:469705"
     Region          830..885
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1114..1205
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1209
                     /gene="LOC143305751"
                     /coded_by="XM_076690553.1:1..3630"
                     /db_xref="GeneID:143305751"
ORIGIN      
        1 mntiasssin itfssihsdl kkdltclavp riadsvpeev fprelikipa nlrladpefh
       61 iprpvdilig sgatlsllsi gqiklsrdds dlflqktrlg wvivggagdn aekqavscal
      121 lelkeqiakf wlienidtet tglseealce thyrenttrd asgryivrlp frtgdrdfgd
      181 srrsafrrfn alqrklnana vlkaeytkvm qeyitlghms raddetvagy ymphhavikt
      241 tsattkvrvv fdasaksetn islndrlmig ptiqdklfeh lvrfrthayv ltadiekmyr
      301 qilvhpqdrk yqrifwyhed rvktyemntv tfgvssapyl airtiqqlad dertdfpyas
      361 eilkrdlyvd dlltgansle eilrirdeii ellkrggfni rqwasnhnha ldninekilg
      421 tdcvtddstv kktlglgwna redklvysvk sfelpakftk rvilseiaki fdplgllgpv
      481 ilaakvfmqe cwkaklewde svpqalcssw tafssqlhfv nnltvdrrli lknasniqih
      541 gfcdaskvgy gaciylrscd qdghtlvrls caksrvaplk emtmprlelc galtlarlyr
      601 etrsaidipi nrvtfwtdst ivlqwlrksp svlrlfesnr vaeiqktvea ewrhvrtkdn
      661 padalsrgqy paafcqnnsw fegpnwlrgr edswpinsvl tvtdlpglkk mtclaltink
      721 cdffinfssy pklvrvvayc lrmlrsnshr gelcaeekia aersiiraiq reqfsseiqh
      781 isssggakgn rlaslnpfid rdgllrvggr lqnaeisysq khpillpsyh hvtdliirdt
      841 hertyhagiq stlytirhrf wlldgknqvr kivrrcvrci rfratpvqrv mgnlpksrve
      901 eaaafthtgv dffgplfvke kkhrnrnrvk aygcvfvcms vkavhieivs dlttegflga
      961 frrfigrrai pthvysdngt nfvgannqlr elyalfgsdq fkgqvnefav sknitwhfnp
     1021 plsphfggiw eaavksfkhh fkrvvgerlf tfeeintfai eieailnsrp lcpissdpnd
     1081 plaltpahvl vgrpltilpe dnllsvpenr lsswrliika rqdfwkrwyt eylnelqkrq
     1141 kwleargeik vdtivllmdk nlpcmqwrlg rvleihpggd gevrvatikg angvfkrnis
     1201 vlcplledv
//
LOCUS       XP_076546669             427 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305752 [Osmia lignaria lignaria].
ACCESSION   XP_076546669
VERSION     XP_076546669.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690554.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="uncharacterized protein LOC143305752"
                     /calculated_mol_wt=48534
     Region          126..264
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..427
                     /gene="LOC143305752"
                     /coded_by="XM_076690554.1:1..1284"
                     /db_xref="GeneID:143305752"
ORIGIN      
        1 mdrirvinrr rttlktqvtq lvnnvasgrf nklvlksrla rvtdmltaye elndelaiid
       61 ptndrlddil eiqdqyyeca gaiegmedag asgartnetl gntglnntli etrrtmklpv
      121 aelptfdgnf dewlsykntf ltmidartdi tdlerfiylk nslrgealkk viifsasven
      181 yksawqllad syekrrilis rhlnaildmk rlttatpsgl tkliddvrqh lsmlktldie
      241 pdhtmvicvl ekalpsdlrq kweelldedv fpslekfykf vsatanrlhh leevdsceks
      301 anqsgnkrrs drepraaktr kteasarsfv tatsiscfcc kqnhniykcp tfqkmttsqr
      361 wdtvkrkqlc rnclrphage ckgsrcrfcg rfhntllhsf anqqssagps tsqakpgiet
      421 tdqtkss
//
LOCUS       XP_076546670             594 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305753 [Osmia lignaria lignaria].
ACCESSION   XP_076546670
VERSION     XP_076546670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690555.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..594
                     /product="uncharacterized protein LOC143305753"
                     /calculated_mol_wt=66530
     Region          134..273
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          446..>568
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     CDS             1..594
                     /gene="LOC143305753"
                     /coded_by="XM_076690555.1:1..1785"
                     /db_xref="GeneID:143305753"
ORIGIN      
        1 mssleeliqk qtqtmrsier alanfkklgq skmtaattqn rmamlkeafl qcqdlhakia
       61 anadprtkts asyfvedqfs iceeryqeaq dfmaetlykv spspdnsapn gsfsnsmtsr
      121 ppathlprih lptfegafee wesfrdrfts mvinndtlsn vdrlhflcss lkgdasravs
      181 hlpvtennfe vawqiitsry dnkrrlvsth lntlfslpsv ssetpselrs lrdninisiq
      241 mlrnlqrpte awsdiivflg sqkldrasrk awefnlgdst efptyeefdt flekrirale
      301 alptskrekp nesdkvkhlk skplsinstt tykilcavck qnhllyqcpt flaktpsery
      361 nilkqqkrcv ncfsvkhaav qctsthhcke cqqkhhtllh frtsnearat pqgtssntse
      421 egveissnfa fkspihtril lstarvrvhs lagrcviara lldqgsaatl isehlaqslr
      481 lervrrttqi tgigeiqsva rhvadilitp atknepayst taivmtaltr yvpsrnnsia
      541 tlshlsglkl adedpmssdp idliigadiy gqlllhevrq gsactptaqh ttlg
//
LOCUS       XP_076546671             431 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305754 [Osmia lignaria lignaria].
ACCESSION   XP_076546671
VERSION     XP_076546671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690556.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..431
                     /product="uncharacterized protein LOC143305754"
                     /calculated_mol_wt=49330
     Region          126..344
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(191..196,231..232,267,269..270,335..336)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(191..196,231,269)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            232
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     CDS             1..431
                     /gene="LOC143305754"
                     /coded_by="XM_076690556.1:1..1296"
                     /db_xref="GeneID:143305754"
ORIGIN      
        1 mssvnihhis llealdqnlk rfweledlpg kvmltpeeqk ceshfretht rtpegryvvr
       61 lpfktdppie lgesrlgalr slrsqenrlh qnqttyqeyc nflseyeslg hmreinqahi
      121 tsnnptyyip hhavfrstsa ttrlrvvfna srrtsngksl ndhllsgpkl qkdlaailvr
      181 wrqfqyvytt diakmyrqil idsrdrdfqr ilwrndqtdp vkeyqlltvt ygttsapyla
      241 lrflqqlnqd agfnypdasy vlnnqayvdd flfggdsiqs lrhirnqtie llkrggfslr
      301 kwasndstll sdidptdhgl alskslnate tvsvlgliwn paldsfsvps snsnqlriyq
      361 tnnpvcdykn frshgmdrps cninkdshpk amvselrmgr scppgkphkm agilsatsgv
      421 rkyfdspknl k
//
LOCUS       XP_076546672             633 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305755 [Osmia lignaria lignaria].
ACCESSION   XP_076546672
VERSION     XP_076546672.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690557.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="uncharacterized protein LOC143305755"
                     /calculated_mol_wt=72091
     Region          248..299
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          528..621
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..633
                     /gene="LOC143305755"
                     /coded_by="XM_076690557.1:1..1902"
                     /db_xref="GeneID:143305755"
ORIGIN      
        1 mlerfrrysw tdssitltwv sqspsrwktf vanrvskiqe lipnahwhhv stddnpadca
       61 srgisieqlk thrlwwcgps wlredqtawp rsfpqstpda pleerthdvt cmavqaitwd
      121 lahrysswpk llrvtamiqi firklrtrte qkgfctqtnp nlsikstpai lapddvrqae
      181 llwlrqmqqe mfpeelktlv ssrtvkksss irtlapyida nglirakerl ahsqlpgeak
      241 tpiilrshpl ltliiehthr kllhagpqlt laclrnqywi vhsrttvrav lykcvpctre
      301 ratvphelmg dlpaprvnqv erafihtgvd yagpiqvrtt pgrehkstka yiavficlti
      361 kaihielvsd ysssaflaay qrfvsrrglp aamysdngtt fqganrelas ayhsaiqnsd
      421 lrnhlassgv awhfmppnap hfgglweaav kslkhhmkrt igsytltfee lstllcriea
      481 clnsrplapm seciddcqal tpghfligtp lvappepsvl dlrdnrlsrw qliqklteif
      541 wkswqndylh tlhqrpkwqi vkkllkvgql vlirnpnrpp skwelgrita chpgedglvr
      601 vvtvktarse ykrpisrlcf lpipingetn ntt
//
LOCUS       XP_076546673             594 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305756 [Osmia lignaria lignaria].
ACCESSION   XP_076546673
VERSION     XP_076546673.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690558.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..594
                     /product="uncharacterized protein LOC143305756"
                     /calculated_mol_wt=66530
     Region          134..273
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          446..>568
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     CDS             1..594
                     /gene="LOC143305756"
                     /coded_by="XM_076690558.1:1..1785"
                     /db_xref="GeneID:143305756"
ORIGIN      
        1 mssleeliqk qtqtmrsier alanfkklgq skmtaattqn rmamlkeafl qcqdlhakia
       61 anadprtkts asyfvedqfs iceeryqeaq dfmaetlykv spspdnsapn gsfsnsmtsr
      121 ppathlprih lptfegafee wesfrdrfts mvinndtlsn vdrlhflcss lkgdasravs
      181 hlpvtennfe vawqiitsry dnkrrlvsth lntlfslpsv ssetpselrs lrdninisiq
      241 mlrnlqrpte awsdiivflg sqkldrasrk awefnlgdst efptyeefdt flekrirale
      301 alptskrekp nesdkvkhlk skplsinstt tykilcavck qnhllyqcpt flaktpsery
      361 nilkqqkrcv ncfsvkhaav qctsthhcke cqqkhhtllh frtsnearat pqgtssntse
      421 egveissnfa fkspihtril lstarvrvhs lagrcviara lldqgsaatl isehlaqslr
      481 lervrrttqi tgigeiqsva rhvadilitp atknepayst taivmtaltr yvpsrnnsia
      541 tlshlsglkl adedpmssdp idliigadiy gqlllhevrq gsactptaqh ttlg
//
LOCUS       XP_076546674             431 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305757 [Osmia lignaria lignaria].
ACCESSION   XP_076546674
VERSION     XP_076546674.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690559.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..431
                     /product="uncharacterized protein LOC143305757"
                     /calculated_mol_wt=49288
     Region          126..344
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(191..196,231..232,267,269..270,335..336)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(191..196,231,269)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            232
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     CDS             1..431
                     /gene="LOC143305757"
                     /coded_by="XM_076690559.1:1..1296"
                     /db_xref="GeneID:143305757"
ORIGIN      
        1 mssvnihhis llealdqnlk rfweledlpg kvmltpeeqk ceshfretht rtpegryvvr
       61 lpfktdppie lgesrlgalr slrsqenrlh qnqttyqeyc nflseyeslg hmreinqahi
      121 tsnnptyyip hhavfrstsa ttrlrvvfna srrtsngksl ndhllsgpkl qkdlaailgr
      181 wrqfqyvytt diakmyrqil idsrdrdfqr ilwrndqtdp vkeyqlltvt ygttsapyla
      241 lrflqqlnqd agfnypdasy vlnnqayvdd flfggdsiqs lrhirnqtie llkrggfslr
      301 kwasndstll sdidptdhgl alskslnate tvsvlgliwn paldsfsvps snsnqlriyq
      361 tnnpvcdykn frshgmdrps cninkdshpk amvselrmgr scppgkphkm agilsatsgv
      421 rkyfdspknl k
//
LOCUS       XP_076546675             633 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305758 [Osmia lignaria lignaria].
ACCESSION   XP_076546675
VERSION     XP_076546675.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690560.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="uncharacterized protein LOC143305758"
                     /calculated_mol_wt=72091
     Region          248..299
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          528..621
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..633
                     /gene="LOC143305758"
                     /coded_by="XM_076690560.1:1..1902"
                     /db_xref="GeneID:143305758"
ORIGIN      
        1 mlerfrrysw tdssitltwv sqspsrwktf vanrvskiqe lipnahwhhv stddnpadca
       61 srgisieqlk thrlwwcgps wlredqtawp rsfpqstpda pleerthdvt cmavqaitwd
      121 lahrysswpk llrvtamiqi firklrtrte qkgfctqtnp nlsikstpai lapddvrqae
      181 llwlrqmqqe mfpeelktlv ssrtvkksss irtlapyida nglirakerl ahsqlpgeak
      241 tpiilrshpl ltliiehthr kllhagpqlt laclrnqywi vhsrttvrav lykcvpctre
      301 ratvphelmg dlpaprvnqv erafihtgvd yagpiqvrtt pgrehkstka yiavficlti
      361 kaihielvsd ysssaflaay qrfvsrrglp aamysdngtt fqganrelas ayhsaiqnsd
      421 lrnhlassgv awhfmppnap hfgglweaav kslkhhmkrt igsytltfee lstllcriea
      481 clnsrplapm seciddcqal tpghfligtp lvappepsvl dlrdnrlsrw qliqklteif
      541 wkswqndylh tlhqrpkwqi vkkllkvgql vlirnpnrpp skwelgrita chpgedglvr
      601 vvtvktarse ykrpisrlcf lpipingetn ntt
//
LOCUS       XP_034194634             738 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610895 [Osmia lignaria lignaria].
ACCESSION   XP_034194634
VERSION     XP_034194634.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338743.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194634.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..738
                     /product="uncharacterized protein LOC117610895"
                     /calculated_mol_wt=82919
     Region          6..164
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Site            23..25
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            23
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Region          284..496
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(348..353,387..388,423,425..426,487..488)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(348..353,387,425)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            388
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          511..671
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..738
                     /gene="LOC117610895"
                     /coded_by="XM_034338743.2:1..2217"
                     /db_xref="GeneID:117610895"
ORIGIN      
        1 mataiiyvmd rdqkpiecrt lidtcsnanf itediasklr lpakqhttti ealnqlstvt
       61 tqivstsiks rlsnfkrtlt citiplisgp ipdqqidrgk ipipaninla dphfhrptsv
      121 dmllgidtal aclsvgqidi spsnktdlil qktlfgwiig gslstsmarh kvflttpnfd
      181 lkkfweleeg pqvrylsaee qeceehfrnh tkrdksgryi valpfnekyt llgesrsral
      241 nrflslqrkf nrepklkcey anviqeyqdl ghmsevqpne rddfylphha vikpssattk
      301 vrvvfdgsak tssglslndp lmvgptiqdd ivlllirfrm hafvltgdie kmyrqflvrp
      361 egrryqrilw rsddgkvkty nlntvtfglt sapylaircl hqladdegnr npaaarvikq
      421 dlyvddlltg adtcggamnl rdgiidilkr gglnirqwas nepqllsgld eaqiskkflg
      481 dstlktlgvi wdaqndkily tidpivanrv tkrtilssia kifdplglla pviikakilm
      541 qrlwqlqldw deatpanlht ewitfaaefn flnncsfdrh vlqhvvsktg lhgfcdaser
      601 aygaciyvrt vtksgnikte llcaksrvap lktvslarle lcgavllsfl yntvtkatsr
      661 nfdtstfwtd stivlnwink epstlktfva nritevqskt dinawkhvks adnpadlisr
      721 gqspsqflad siwrhgpq
//
LOCUS       XP_034194632             257 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610893 [Osmia lignaria lignaria].
ACCESSION   XP_034194632
VERSION     XP_034194632.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338741.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194632.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..257
                     /product="uncharacterized protein LOC117610893"
                     /calculated_mol_wt=29494
     Region          <1..59
                     /region_name="rve"
                     /note="Integrase core domain; pfam00665"
                     /db_xref="CDD:459897"
     Region          158..251
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..257
                     /gene="LOC117610893"
                     /coded_by="XM_034338741.2:1..774"
                     /db_xref="GeneID:117610893"
ORIGIN      
        1 mttealiaal krfiaqrgic qniysdngtn fvgvsneiid ihrtlqrddk vrhfltdkri
       61 swhfmpalsp nfgglweaav kcfkhhlkrv vgeelftfeq fntfvieiea ilnsrpltpl
      121 ssdpndpsal tpahflisss ltsmpevdfs etpsnrlskw qhiqkvkqnf wsrwykeyih
      181 qlnvrqkwtk gsheikegsl vvlkddhlpp lqwhlgriee vhlgqdnvir avtvrgpsgv
      241 ykrnvkqlal lpiqevv
//
LOCUS       XP_076546676             213 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610914 [Osmia lignaria lignaria].
ACCESSION   XP_076546676
VERSION     XP_076546676.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690561.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 27% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..213
                     /product="uncharacterized protein LOC117610914"
                     /calculated_mol_wt=24326
     CDS             1..213
                     /gene="LOC117610914"
                     /coded_by="XM_076690561.1:1..642"
                     /db_xref="GeneID:117610914"
ORIGIN      
        1 mleersknps vnnkdifdni chtnldaaty isynsmksvm srekrksrpg lpktvqsahe
       61 ilkdydvlke iykgcaisnd nkyalifsnt tlltalenak evymdgtfsv vprmppfhrl
      121 ytvhiryndt giatifalce crtksmykai wgkivslvpr lqdnletvmc gyeraamesv
      181 qaqfprftih gcwfhftqal lrkwkklglq gap
//
LOCUS       XP_076546677             413 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305759 [Osmia lignaria lignaria].
ACCESSION   XP_076546677
VERSION     XP_076546677.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690562.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..413
                     /product="uncharacterized protein LOC143305759"
                     /calculated_mol_wt=46985
     Region          <36..159
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     Site            order(43,78,152)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197311"
     CDS             1..413
                     /gene="LOC143305759"
                     /coded_by="XM_076690562.1:1..1242"
                     /db_xref="GeneID:143305759"
ORIGIN      
        1 mrlrekrara tklaefctrd qatvllpaga ggsganlvvc stylpyvsev ppptrelrel
       61 vdyckakglq llvgckaneh yivcdssnin nrgeaqleyl sttdleilnr gssstfvtsr
      121 rqevlditlg smktaqaiqd wrvndeatlp shrlimfklt tetylsvtet yrnprstlwf
      181 qyretlkeel rghcvrprta egveievasl haaitrsyer scpakqrres rrvpwwnnel
      241 nklrararkl lnkamkatte aawdlykeaq kaykklisap krkswknfcq etdalpmvar
      301 lrkvfvlgsp qkskglklpn ggvtvdppqt lrhlveshfp gselvpeeqf qeervplrns
      361 lrdwdlaskv vtmdrlrwai nsfsrnkspg vdgtfpallq etkedllrhl cni
//
LOCUS       XP_076546678             107 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610927 [Osmia lignaria lignaria].
ACCESSION   XP_076546678
VERSION     XP_076546678.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690563.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..107
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..107
                     /product="uncharacterized protein LOC117610927"
                     /calculated_mol_wt=12098
     CDS             1..107
                     /gene="LOC117610927"
                     /coded_by="XM_076690563.1:92..415"
                     /db_xref="GeneID:117610927"
ORIGIN      
        1 mvakqqqhll hqhhgipskp aflpisslag mtefqnided syrevvnyle yvggfnlkea
       61 vtlsfketvs dclltsyswl grednnlalc etrivhaihv filyayi
//
LOCUS       XP_034194672             697 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117610928 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194672
VERSION     XP_034194672.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338781.2
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194672.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 10% of CDS bases
            frameshifts          :: corrected 1 indel
            internal stop codons :: corrected 2 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117610928"
                     /calculated_mol_wt=77526
     Region          <189..308
                     /region_name="Transposase_21"
                     /note="Transposase family tnp2; cl29371"
                     /db_xref="CDD:475188"
     CDS             1..697
                     /gene="LOC117610928"
                     /coded_by="XM_034338781.2:1..2094"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: inserted 1 base in 1 codon;
                     substituted 2 bases at 2 genomic stop codons"
                     /db_xref="GeneID:117610928"
ORIGIN      
        1 mssfnfklsv srkpifkiss trqrrnirne vgsnsvvssl aspsssciqe inapvnladv
       61 pssstdyadg idaihgadvd dssssssssl sssvlcssea vpetsfqesl asysvdnnin
      121 hvqgnnlltl lrthtsftnl pkdirtvlgt prtrvstyvv epgeyvhfdv eaeivkyirn
      181 asfspdrele ldfstdgcal draxtihlwp iqcmtrnipd arpivvgiyr glqkpsdpnr
      241 lfnmfimnit hmlsnggvdl dgtkvpiklr cfiadapart filnhrghts srpcskckvs
      301 gthsegryvf ngikhvlrtd nqysqcvded hhkegcspla slpfgmvsqv sfeymhlvcl
      361 gvtkkilsaw isgkysrlsk lsgshlagms arldrlrefx psefgrpsrt lvyyakykat
      421 efrqfllytg pvvaygllnd nlythflflh tairilasqs pssqflnfae lglqkfvlrs
      481 eelygpnfcs ynvhgllhlt ndvrrlgpvd sfsafpyesn mrifrkycxk pglpvqqfvn
      541 rmaeiqahga nrarvvassd iavtmplgcg tdilqyrriq fngmffsldi rdnccilcdg
      601 aicilssiae drntfrlgvq kftevgdifd igikssavgl ykcrylspef nispydvcsk
      661 cfrmpfpgdn mddneedeqe rreyavvvml hsdnmlh
//
LOCUS       XP_076546679             362 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610679 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546679
VERSION     XP_076546679.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690564.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..362
                     /product="uncharacterized protein LOC117610679 isoform X1"
                     /calculated_mol_wt=42265
     CDS             1..362
                     /gene="LOC117610679"
                     /coded_by="XM_076690564.1:544..1632"
                     /db_xref="GeneID:117610679"
ORIGIN      
        1 mlrsdesfkl rkqeehhkit sniedlnigm vtqfpldymh lvllgvmkkl lqfwikgkhn
       61 vrmvatqitl astsllsmss flpkefarkp rkldevdrfk atelrqllly tgpiiffkkl
      121 srdkylhfls lsvaiqilcn keycvqlldy ahslllyfve nygtlygrqh isynvhnlih
      181 lcsyvklwge ldkfsafkfe nymqkiksri rsssrpleql vnrckeednc nitniernyp
      241 ilkeliingi kiiksvqckg fylssrtadn ccllldgtti simkiyfknk eiyiigkkyl
      301 niksffhipc nsfnigikki nesqhelitv plsdiklkcv klplcdkehv vlpllhqqei
      361 cl
//
LOCUS       XP_034194208             360 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610679 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194208
VERSION     XP_034194208.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338317.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194208.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="uncharacterized protein LOC117610679 isoform X2"
                     /calculated_mol_wt=40985
     Region          237..311
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..360
                     /gene="LOC117610679"
                     /coded_by="XM_034338317.2:367..1449"
                     /db_xref="GeneID:117610679"
ORIGIN      
        1 mvknfavvtf psdgnneeei vsevpslwls snltecwwps vknintfivk qtppvnndpk
       61 wtlhpikfhg hydsldearq ratnysssde sntnqikksc ikrslntvyn sesesdsddp
      121 rsiipnppnl kdtnvsqsek niqsisnild stiinnidme ildgtivphk siqdvytlvs
      181 gmsdkmednf ekiytaitti slniqdidtr lknmetqhnq hkskasamdt sniqksfpfq
      241 nineviafen slsentdeyn kfmlyisrig ggsakenlir iyrtifsnev akvsswkglr
      301 nhfkisslks mltgiqattl aqyqftnkef editkewfrq ggqrlsrqqk yseevnpqai
//
LOCUS       XP_034194210             360 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610679 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194210
VERSION     XP_034194210.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338319.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194210.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="uncharacterized protein LOC117610679 isoform X2"
                     /calculated_mol_wt=40985
     Region          237..311
                     /region_name="DUF4806"
                     /note="Domain of unknown function (DUF4806); pfam16064"
                     /db_xref="CDD:465001"
     CDS             1..360
                     /gene="LOC117610679"
                     /coded_by="XM_034338319.2:139..1221"
                     /db_xref="GeneID:117610679"
ORIGIN      
        1 mvknfavvtf psdgnneeei vsevpslwls snltecwwps vknintfivk qtppvnndpk
       61 wtlhpikfhg hydsldearq ratnysssde sntnqikksc ikrslntvyn sesesdsddp
      121 rsiipnppnl kdtnvsqsek niqsisnild stiinnidme ildgtivphk siqdvytlvs
      181 gmsdkmednf ekiytaitti slniqdidtr lknmetqhnq hkskasamdt sniqksfpfq
      241 nineviafen slsentdeyn kfmlyisrig ggsakenlir iyrtifsnev akvsswkglr
      301 nhfkisslks mltgiqattl aqyqftnkef editkewfrq ggqrlsrqqk yseevnpqai
//
LOCUS       XP_034195637            2463 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195637
VERSION     XP_034195637.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339746.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195637.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2463
                     /product="nuclear receptor corepressor smrter isoform X1"
                     /calculated_mol_wt=265958
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1492..1893
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1915..>2029
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2463
                     /gene="Smr"
                     /coded_by="XM_034339746.2:2272..9663"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssateta
     1381 degqgstdle nssvvvtltt psngtplqqh qqshqqvppp shsppsttsn snpltvkdlm
     1441 lgviemqlkr npnspagtgg ssvpvnsgtp tissilrtdh rnditfvrey kpsstmants
     1501 mprdsnlatl svvsgphhgh rsapspqqig qiqatitpcv papsssqsst sdilpkeglv
     1561 vmqvqqalre tegtldlsik kprqqeynhs lqtlhkpptv tlyrpepppg ayyhphshpe
     1621 qgrgakspqv yassprpqap ltpkmnkvav ppphpklspk lgamgttghk sgsithgtpv
     1681 ssaryegllr qmtppgnpgs vagtpsvtsg psavnapssq gpketgsitq gtpvhpfavd
     1741 krapmydyhr tirpspvttg nipgqgqsqa gtaavvvths nqynsysgrp ppsytmeqql
     1801 ssrqiiindy itsqqmhars rsgvtsesss knevpstlyy tntpppqtht qprqgviqrh
     1861 npqkqihypp pppgleafss lvdvavqqps lpvphphshp asgssshegl gktmadrlla
     1921 drygdkhrla ihqdhrmava hhhqrdrere rdlvhlreke eyrlqrekei lqqdhrmaqq
     1981 rekdsqhadh rltvhqqrek eihqqmavaa aqrekehqeh rlavqqqrer dlahlqqqqq
     2041 qqqqqhlqqh iqqqrmeaer rhmvqlyrdq qqldrdsrli ssgftqsrlq qpqqsqqqqq
     2101 qqqqqqqsss rqsqssgqqq nesastltaa slidaiithq inqsventgg nsqpprpgdr
     2161 lfqgfhreas iepngmahsp agegnggnng pgsasgngng nkpitlgehv dhivskdygp
     2221 phssyrsysg yqisedqwkr rkaneadsvk agderqiirv tqqqsqqqqq qqqqqqqqqq
     2281 qhqhqhqqta ssvgkqqfhs vepvsppeag tnhytrrmye tttatstsgt psnkphlspl
     2341 dyvknkivev mrtseddkgg nagdrnggnv tsgdkdekig gnvespssge mmvdespnqt
     2401 pqppapaptt tfypfsalgv htgpppappp ptsgstsvtk seqmqaepap llsaqyepls
     2461 ded
//
LOCUS       XP_076546680            2463 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546680
VERSION     XP_076546680.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690565.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2463
                     /product="nuclear receptor corepressor smrter isoform X1"
                     /calculated_mol_wt=265958
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1492..1893
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1915..>2029
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2463
                     /gene="Smr"
                     /coded_by="XM_076690565.1:2201..9592"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssateta
     1381 degqgstdle nssvvvtltt psngtplqqh qqshqqvppp shsppsttsn snpltvkdlm
     1441 lgviemqlkr npnspagtgg ssvpvnsgtp tissilrtdh rnditfvrey kpsstmants
     1501 mprdsnlatl svvsgphhgh rsapspqqig qiqatitpcv papsssqsst sdilpkeglv
     1561 vmqvqqalre tegtldlsik kprqqeynhs lqtlhkpptv tlyrpepppg ayyhphshpe
     1621 qgrgakspqv yassprpqap ltpkmnkvav ppphpklspk lgamgttghk sgsithgtpv
     1681 ssaryegllr qmtppgnpgs vagtpsvtsg psavnapssq gpketgsitq gtpvhpfavd
     1741 krapmydyhr tirpspvttg nipgqgqsqa gtaavvvths nqynsysgrp ppsytmeqql
     1801 ssrqiiindy itsqqmhars rsgvtsesss knevpstlyy tntpppqtht qprqgviqrh
     1861 npqkqihypp pppgleafss lvdvavqqps lpvphphshp asgssshegl gktmadrlla
     1921 drygdkhrla ihqdhrmava hhhqrdrere rdlvhlreke eyrlqrekei lqqdhrmaqq
     1981 rekdsqhadh rltvhqqrek eihqqmavaa aqrekehqeh rlavqqqrer dlahlqqqqq
     2041 qqqqqhlqqh iqqqrmeaer rhmvqlyrdq qqldrdsrli ssgftqsrlq qpqqsqqqqq
     2101 qqqqqqqsss rqsqssgqqq nesastltaa slidaiithq inqsventgg nsqpprpgdr
     2161 lfqgfhreas iepngmahsp agegnggnng pgsasgngng nkpitlgehv dhivskdygp
     2221 phssyrsysg yqisedqwkr rkaneadsvk agderqiirv tqqqsqqqqq qqqqqqqqqq
     2281 qhqhqhqqta ssvgkqqfhs vepvsppeag tnhytrrmye tttatstsgt psnkphlspl
     2341 dyvknkivev mrtseddkgg nagdrnggnv tsgdkdekig gnvespssge mmvdespnqt
     2401 pqppapaptt tfypfsalgv htgpppappp ptsgstsvtk seqmqaepap llsaqyepls
     2461 ded
//
LOCUS       XP_076546681            2463 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546681
VERSION     XP_076546681.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690566.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2463
                     /product="nuclear receptor corepressor smrter isoform X1"
                     /calculated_mol_wt=265958
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1492..1893
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1915..>2029
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2463
                     /gene="Smr"
                     /coded_by="XM_076690566.1:2186..9577"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssateta
     1381 degqgstdle nssvvvtltt psngtplqqh qqshqqvppp shsppsttsn snpltvkdlm
     1441 lgviemqlkr npnspagtgg ssvpvnsgtp tissilrtdh rnditfvrey kpsstmants
     1501 mprdsnlatl svvsgphhgh rsapspqqig qiqatitpcv papsssqsst sdilpkeglv
     1561 vmqvqqalre tegtldlsik kprqqeynhs lqtlhkpptv tlyrpepppg ayyhphshpe
     1621 qgrgakspqv yassprpqap ltpkmnkvav ppphpklspk lgamgttghk sgsithgtpv
     1681 ssaryegllr qmtppgnpgs vagtpsvtsg psavnapssq gpketgsitq gtpvhpfavd
     1741 krapmydyhr tirpspvttg nipgqgqsqa gtaavvvths nqynsysgrp ppsytmeqql
     1801 ssrqiiindy itsqqmhars rsgvtsesss knevpstlyy tntpppqtht qprqgviqrh
     1861 npqkqihypp pppgleafss lvdvavqqps lpvphphshp asgssshegl gktmadrlla
     1921 drygdkhrla ihqdhrmava hhhqrdrere rdlvhlreke eyrlqrekei lqqdhrmaqq
     1981 rekdsqhadh rltvhqqrek eihqqmavaa aqrekehqeh rlavqqqrer dlahlqqqqq
     2041 qqqqqhlqqh iqqqrmeaer rhmvqlyrdq qqldrdsrli ssgftqsrlq qpqqsqqqqq
     2101 qqqqqqqsss rqsqssgqqq nesastltaa slidaiithq inqsventgg nsqpprpgdr
     2161 lfqgfhreas iepngmahsp agegnggnng pgsasgngng nkpitlgehv dhivskdygp
     2221 phssyrsysg yqisedqwkr rkaneadsvk agderqiirv tqqqsqqqqq qqqqqqqqqq
     2281 qhqhqhqqta ssvgkqqfhs vepvsppeag tnhytrrmye tttatstsgt psnkphlspl
     2341 dyvknkivev mrtseddkgg nagdrnggnv tsgdkdekig gnvespssge mmvdespnqt
     2401 pqppapaptt tfypfsalgv htgpppappp ptsgstsvtk seqmqaepap llsaqyepls
     2461 ded
//
LOCUS       XP_076546682            2463 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546682
VERSION     XP_076546682.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690567.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2463
                     /product="nuclear receptor corepressor smrter isoform X1"
                     /calculated_mol_wt=265958
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1492..1893
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1915..>2029
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2463
                     /gene="Smr"
                     /coded_by="XM_076690567.1:2409..9800"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssateta
     1381 degqgstdle nssvvvtltt psngtplqqh qqshqqvppp shsppsttsn snpltvkdlm
     1441 lgviemqlkr npnspagtgg ssvpvnsgtp tissilrtdh rnditfvrey kpsstmants
     1501 mprdsnlatl svvsgphhgh rsapspqqig qiqatitpcv papsssqsst sdilpkeglv
     1561 vmqvqqalre tegtldlsik kprqqeynhs lqtlhkpptv tlyrpepppg ayyhphshpe
     1621 qgrgakspqv yassprpqap ltpkmnkvav ppphpklspk lgamgttghk sgsithgtpv
     1681 ssaryegllr qmtppgnpgs vagtpsvtsg psavnapssq gpketgsitq gtpvhpfavd
     1741 krapmydyhr tirpspvttg nipgqgqsqa gtaavvvths nqynsysgrp ppsytmeqql
     1801 ssrqiiindy itsqqmhars rsgvtsesss knevpstlyy tntpppqtht qprqgviqrh
     1861 npqkqihypp pppgleafss lvdvavqqps lpvphphshp asgssshegl gktmadrlla
     1921 drygdkhrla ihqdhrmava hhhqrdrere rdlvhlreke eyrlqrekei lqqdhrmaqq
     1981 rekdsqhadh rltvhqqrek eihqqmavaa aqrekehqeh rlavqqqrer dlahlqqqqq
     2041 qqqqqhlqqh iqqqrmeaer rhmvqlyrdq qqldrdsrli ssgftqsrlq qpqqsqqqqq
     2101 qqqqqqqsss rqsqssgqqq nesastltaa slidaiithq inqsventgg nsqpprpgdr
     2161 lfqgfhreas iepngmahsp agegnggnng pgsasgngng nkpitlgehv dhivskdygp
     2221 phssyrsysg yqisedqwkr rkaneadsvk agderqiirv tqqqsqqqqq qqqqqqqqqq
     2281 qhqhqhqqta ssvgkqqfhs vepvsppeag tnhytrrmye tttatstsgt psnkphlspl
     2341 dyvknkivev mrtseddkgg nagdrnggnv tsgdkdekig gnvespssge mmvdespnqt
     2401 pqppapaptt tfypfsalgv htgpppappp ptsgstsvtk seqmqaepap llsaqyepls
     2461 ded
//
LOCUS       XP_076546683            2463 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546683
VERSION     XP_076546683.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690568.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2463
                     /product="nuclear receptor corepressor smrter isoform X1"
                     /calculated_mol_wt=265958
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1492..1893
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1915..>2029
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2463
                     /gene="Smr"
                     /coded_by="XM_076690568.1:2334..9725"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssateta
     1381 degqgstdle nssvvvtltt psngtplqqh qqshqqvppp shsppsttsn snpltvkdlm
     1441 lgviemqlkr npnspagtgg ssvpvnsgtp tissilrtdh rnditfvrey kpsstmants
     1501 mprdsnlatl svvsgphhgh rsapspqqig qiqatitpcv papsssqsst sdilpkeglv
     1561 vmqvqqalre tegtldlsik kprqqeynhs lqtlhkpptv tlyrpepppg ayyhphshpe
     1621 qgrgakspqv yassprpqap ltpkmnkvav ppphpklspk lgamgttghk sgsithgtpv
     1681 ssaryegllr qmtppgnpgs vagtpsvtsg psavnapssq gpketgsitq gtpvhpfavd
     1741 krapmydyhr tirpspvttg nipgqgqsqa gtaavvvths nqynsysgrp ppsytmeqql
     1801 ssrqiiindy itsqqmhars rsgvtsesss knevpstlyy tntpppqtht qprqgviqrh
     1861 npqkqihypp pppgleafss lvdvavqqps lpvphphshp asgssshegl gktmadrlla
     1921 drygdkhrla ihqdhrmava hhhqrdrere rdlvhlreke eyrlqrekei lqqdhrmaqq
     1981 rekdsqhadh rltvhqqrek eihqqmavaa aqrekehqeh rlavqqqrer dlahlqqqqq
     2041 qqqqqhlqqh iqqqrmeaer rhmvqlyrdq qqldrdsrli ssgftqsrlq qpqqsqqqqq
     2101 qqqqqqqsss rqsqssgqqq nesastltaa slidaiithq inqsventgg nsqpprpgdr
     2161 lfqgfhreas iepngmahsp agegnggnng pgsasgngng nkpitlgehv dhivskdygp
     2221 phssyrsysg yqisedqwkr rkaneadsvk agderqiirv tqqqsqqqqq qqqqqqqqqq
     2281 qhqhqhqqta ssvgkqqfhs vepvsppeag tnhytrrmye tttatstsgt psnkphlspl
     2341 dyvknkivev mrtseddkgg nagdrnggnv tsgdkdekig gnvespssge mmvdespnqt
     2401 pqppapaptt tfypfsalgv htgpppappp ptsgstsvtk seqmqaepap llsaqyepls
     2461 ded
//
LOCUS       XP_076546684            2463 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546684
VERSION     XP_076546684.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690569.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2463
                     /product="nuclear receptor corepressor smrter isoform X1"
                     /calculated_mol_wt=265958
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1492..1893
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1915..>2029
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2463
                     /gene="Smr"
                     /coded_by="XM_076690569.1:2048..9439"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssateta
     1381 degqgstdle nssvvvtltt psngtplqqh qqshqqvppp shsppsttsn snpltvkdlm
     1441 lgviemqlkr npnspagtgg ssvpvnsgtp tissilrtdh rnditfvrey kpsstmants
     1501 mprdsnlatl svvsgphhgh rsapspqqig qiqatitpcv papsssqsst sdilpkeglv
     1561 vmqvqqalre tegtldlsik kprqqeynhs lqtlhkpptv tlyrpepppg ayyhphshpe
     1621 qgrgakspqv yassprpqap ltpkmnkvav ppphpklspk lgamgttghk sgsithgtpv
     1681 ssaryegllr qmtppgnpgs vagtpsvtsg psavnapssq gpketgsitq gtpvhpfavd
     1741 krapmydyhr tirpspvttg nipgqgqsqa gtaavvvths nqynsysgrp ppsytmeqql
     1801 ssrqiiindy itsqqmhars rsgvtsesss knevpstlyy tntpppqtht qprqgviqrh
     1861 npqkqihypp pppgleafss lvdvavqqps lpvphphshp asgssshegl gktmadrlla
     1921 drygdkhrla ihqdhrmava hhhqrdrere rdlvhlreke eyrlqrekei lqqdhrmaqq
     1981 rekdsqhadh rltvhqqrek eihqqmavaa aqrekehqeh rlavqqqrer dlahlqqqqq
     2041 qqqqqhlqqh iqqqrmeaer rhmvqlyrdq qqldrdsrli ssgftqsrlq qpqqsqqqqq
     2101 qqqqqqqsss rqsqssgqqq nesastltaa slidaiithq inqsventgg nsqpprpgdr
     2161 lfqgfhreas iepngmahsp agegnggnng pgsasgngng nkpitlgehv dhivskdygp
     2221 phssyrsysg yqisedqwkr rkaneadsvk agderqiirv tqqqsqqqqq qqqqqqqqqq
     2281 qhqhqhqqta ssvgkqqfhs vepvsppeag tnhytrrmye tttatstsgt psnkphlspl
     2341 dyvknkivev mrtseddkgg nagdrnggnv tsgdkdekig gnvespssge mmvdespnqt
     2401 pqppapaptt tfypfsalgv htgpppappp ptsgstsvtk seqmqaepap llsaqyepls
     2461 ded
//
LOCUS       XP_076546685            2463 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546685
VERSION     XP_076546685.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690570.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2463
                     /product="nuclear receptor corepressor smrter isoform X1"
                     /calculated_mol_wt=265958
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1492..1893
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1915..>2029
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2463
                     /gene="Smr"
                     /coded_by="XM_076690570.1:2184..9575"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssateta
     1381 degqgstdle nssvvvtltt psngtplqqh qqshqqvppp shsppsttsn snpltvkdlm
     1441 lgviemqlkr npnspagtgg ssvpvnsgtp tissilrtdh rnditfvrey kpsstmants
     1501 mprdsnlatl svvsgphhgh rsapspqqig qiqatitpcv papsssqsst sdilpkeglv
     1561 vmqvqqalre tegtldlsik kprqqeynhs lqtlhkpptv tlyrpepppg ayyhphshpe
     1621 qgrgakspqv yassprpqap ltpkmnkvav ppphpklspk lgamgttghk sgsithgtpv
     1681 ssaryegllr qmtppgnpgs vagtpsvtsg psavnapssq gpketgsitq gtpvhpfavd
     1741 krapmydyhr tirpspvttg nipgqgqsqa gtaavvvths nqynsysgrp ppsytmeqql
     1801 ssrqiiindy itsqqmhars rsgvtsesss knevpstlyy tntpppqtht qprqgviqrh
     1861 npqkqihypp pppgleafss lvdvavqqps lpvphphshp asgssshegl gktmadrlla
     1921 drygdkhrla ihqdhrmava hhhqrdrere rdlvhlreke eyrlqrekei lqqdhrmaqq
     1981 rekdsqhadh rltvhqqrek eihqqmavaa aqrekehqeh rlavqqqrer dlahlqqqqq
     2041 qqqqqhlqqh iqqqrmeaer rhmvqlyrdq qqldrdsrli ssgftqsrlq qpqqsqqqqq
     2101 qqqqqqqsss rqsqssgqqq nesastltaa slidaiithq inqsventgg nsqpprpgdr
     2161 lfqgfhreas iepngmahsp agegnggnng pgsasgngng nkpitlgehv dhivskdygp
     2221 phssyrsysg yqisedqwkr rkaneadsvk agderqiirv tqqqsqqqqq qqqqqqqqqq
     2281 qhqhqhqqta ssvgkqqfhs vepvsppeag tnhytrrmye tttatstsgt psnkphlspl
     2341 dyvknkivev mrtseddkgg nagdrnggnv tsgdkdekig gnvespssge mmvdespnqt
     2401 pqppapaptt tfypfsalgv htgpppappp ptsgstsvtk seqmqaepap llsaqyepls
     2461 ded
//
LOCUS       XP_076546686            2462 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546686
VERSION     XP_076546686.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690571.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2462
                     /product="nuclear receptor corepressor smrter isoform X2"
                     /calculated_mol_wt=265829
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1203..1245
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1491..1892
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1914..>2028
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2462
                     /gene="Smr"
                     /coded_by="XM_076690571.1:2272..9660"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkviid gkstssssss lsvnastlqs dlkdsnkkkt gcreansggs
     1021 gtignasrik ekkkdnhatp metsdeeaqs idtiesgrqi gphacavcqn ivegntqsra
     1081 lpprqasqyg lredqvapga rvcntcrcka vrgrytacpl lgcpnlnnts sktkvkrlra
     1141 lpakwfdlpa evrdpiiqef qipnnatkcc sacfnrisrr laphltggte vpedtadsla
     1201 rqwtdeeleq lrralrehgt nwvkvadqip gktnhqckny yfayrkklsl dqvvaeyyqs
     1261 lgeerrpclt deeesgssts scdelavhds sdtasagspa nnmtsgtpgt pgasasanlp
     1321 mpfsrstdqk lgdkladiav vslvppvsis ssqqsstsgg snaasagtre dydssattad
     1381 egqgstdlen ssvvvtlttp sngtplqqhq qshqqvppps hsppsttsns npltvkdlml
     1441 gviemqlkrn pnspagtggs svpvnsgtpt issilrtdhr nditfvreyk psstmantsm
     1501 prdsnlatls vvsgphhghr sapspqqigq iqatitpcvp apsssqssts dilpkeglvv
     1561 mqvqqalret egtldlsikk prqqeynhsl qtlhkpptvt lyrpepppga yyhphshpeq
     1621 grgakspqvy assprpqapl tpkmnkvavp pphpklspkl gamgttghks gsithgtpvs
     1681 saryegllrq mtppgnpgsv agtpsvtsgp savnapssqg pketgsitqg tpvhpfavdk
     1741 rapmydyhrt irpspvttgn ipgqgqsqag taavvvthsn qynsysgrpp psytmeqqls
     1801 srqiiindyi tsqqmharsr sgvtsesssk nevpstlyyt ntpppqthtq prqgviqrhn
     1861 pqkqihyppp ppgleafssl vdvavqqpsl pvphphshpa sgsssheglg ktmadrllad
     1921 rygdkhrlai hqdhrmavah hhqrdrerer dlvhlrekee yrlqrekeil qqdhrmaqqr
     1981 ekdsqhadhr ltvhqqreke ihqqmavaaa qrekehqehr lavqqqrerd lahlqqqqqq
     2041 qqqqhlqqhi qqqrmeaerr hmvqlyrdqq qldrdsrlis sgftqsrlqq pqqsqqqqqq
     2101 qqqqqqsssr qsqssgqqqn esastltaas lidaiithqi nqsventggn sqpprpgdrl
     2161 fqgfhreasi epngmahspa gegnggnngp gsasgngngn kpitlgehvd hivskdygpp
     2221 hssyrsysgy qisedqwkrr kaneadsvka gderqiirvt qqqsqqqqqq qqqqqqqqqq
     2281 hqhqhqqtas svgkqqfhsv epvsppeagt nhytrrmyet ttatstsgtp snkphlspld
     2341 yvknkivevm rtseddkggn agdrnggnvt sgdkdekigg nvespssgem mvdespnqtp
     2401 qppapapttt fypfsalgvh tgpppapppp tsgstsvtks eqmqaepapl lsaqyeplsd
     2461 ed
//
LOCUS       XP_034195638            2428 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195638
VERSION     XP_034195638.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339747.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195638.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2428
                     /product="nuclear receptor corepressor smrter isoform X3"
                     /calculated_mol_wt=262376
     Region          461..550
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <477..>694
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          747..790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(747,776..777,779..780,782..784,786..788)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1168..1210
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1590..1858
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1880..>1994
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2428
                     /gene="Smr"
                     /coded_by="XM_034339747.2:2271..9557"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 metvgkqvqv vsglgvggpv gpvgpvggdl hhhhhphphs hpphphahph ghphgthghs
       61 lvgvtstpgr gqtqppvshn qhlparstpv qlhrptqnyv nikssspvsp rtvgtavtyv
      121 qggaaassaa aaagtvgsgt aggpsigggg ggggsgggvg vsnasgansg tgvvgagsga
      181 ggavgnvgaa gsgnssgtsn saagagsnsg gnsgtvsgap ggyvavpmag glryihpypp
      241 tptsasvpav atvvtsassa ptpvpapaaa panpptvsqt pppslsgtay tardayrsat
      301 tnvneriais vsssplsqig vgvgvvtaey aansgigsam aggrgdrpri sllppasvtp
      361 tpsptapdyq hvssvrnsri glmpvqpdqy csrtaieman lqeappfkki rlgqptqvqs
      421 qsqsqsrcqs lspadpcvkq ehvvqlqqpl ridtrpttga ytpqteaisp tlpepntqed
      481 aqfrstkddl lqqiakvdre iakresqink lrkklkelee tankpleang lkrqveeqsq
      541 qpkhqslaqk iyaenrrkae eahrllerlg pkvelplynq psdtsvyqen rtkhqtcmra
      601 rliarlrreh aeraslhrqq sqtyailvqe whrkverlea tqkrkskeak nreffekvfp
      661 elrkqredke rfnrvgarik seadleeimd glqeqemedk kmrsyavipp llldtkqrri
      721 afqnrngllq peelealhse rklinvwsav ehelfkekyl qhpknfgtia qslehksvpd
      781 cvhhyyltkk aenykqllrk srqrtrssrn npsnkeknnl ppsigpidil stgvttrlqr
      841 eqqqktqenp qsnsaatttt ttttttttss vstsaattiv ttaatalstp tssicistes
      901 viasttsatt tsvpsittcv ttsatsatts akdnretnke nketkseske qhlvkvevke
      961 epqwlnsetv agkdvkvmea nsggsgtign asrikekkkd nhatpmetsd eeaqsidtie
     1021 sgrqigphac avcqnivegn tqsralpprq asqyglredq vapgarvcnt crckavrgry
     1081 tacpllgcpn lnntssktkv krlralpakw fdlpaevrdp iiqefqipnn atkccsacfn
     1141 risrrlaphl tggtevpedt adslarqwtd eeleqlrral rehgtnwvkv adqipgktnh
     1201 qcknyyfayr kklsldqvva eyyqslgeer rpcltdeees gsstsscdel avhdssdtas
     1261 agspannmts gtpgtpgasa sanlpmpfsr stdqklgdkl adiavvslvp pvsisssqqs
     1321 stsggsnaas agtredydss atetadegqg stdlenssvv vtlttpsngt plqqhqqshq
     1381 qvpppshspp sttsnsnplt vkdlmlgvie mqlkrnpnsp agtggssvpv nsgtptissi
     1441 lrtdhrndit fvreykpsst mantsmprds nlatlsvvsg phhghrsaps pqqigqiqat
     1501 itpcvpapss sqsstsdilp keglvvmqvq qalretegtl dlsikkprqq eynhslqtlh
     1561 kpptvtlyrp epppgayyhp hshpeqgrga kspqvyassp rpqapltpkm nkvavppphp
     1621 klspklgamg ttghksgsit hgtpvssary egllrqmtpp gnpgsvagtp svtsgpsavn
     1681 apssqgpket gsitqgtpvh pfavdkrapm ydyhrtirps pvttgnipgq gqsqagtaav
     1741 vvthsnqyns ysgrpppsyt meqqlssrqi iindyitsqq mharsrsgvt sesssknevp
     1801 stlyytntpp pqthtqprqg viqrhnpqkq ihypppppgl eafsslvdva vqqpslpvph
     1861 phshpasgss sheglgktma drlladrygd khrlaihqdh rmavahhhqr drererdlvh
     1921 lrekeeyrlq rekeilqqdh rmaqqrekds qhadhrltvh qqrekeihqq mavaaaqrek
     1981 ehqehrlavq qqrerdlahl qqqqqqqqqq hlqqhiqqqr meaerrhmvq lyrdqqqldr
     2041 dsrlissgft qsrlqqpqqs qqqqqqqqqq qqsssrqsqs sgqqqnesas tltaaslida
     2101 iithqinqsv entggnsqpp rpgdrlfqgf hreasiepng mahspagegn ggnngpgsas
     2161 gngngnkpit lgehvdhivs kdygpphssy rsysgyqise dqwkrrkane adsvkagder
     2221 qiirvtqqqs qqqqqqqqqq qqqqqqhqhq hqqtassvgk qqfhsvepvs ppeagtnhyt
     2281 rrmyetttat stsgtpsnkp hlspldyvkn kivevmrtse ddkggnagdr nggnvtsgdk
     2341 dekiggnves pssgemmvde spnqtpqppa paptttfypf salgvhtgpp papppptsgs
     2401 tsvtkseqmq aepapllsaq yeplsded
//
LOCUS       XP_076546687            2388 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor corepressor smrter isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546687
VERSION     XP_076546687.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690572.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2388
                     /product="nuclear receptor corepressor smrter isoform X4"
                     /calculated_mol_wt=258506
     Region          386..475
                     /region_name="GPS2_interact"
                     /note="G-protein pathway suppressor 2-interacting domain;
                     pfam15784"
                     /db_xref="CDD:464868"
     Region          <402..>619
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          672..715
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cl21498"
                     /db_xref="CDD:473887"
     Site            order(672,701..702,704..705,707..709,711..713)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:212559"
     Region          1128..1170
                     /region_name="Myb_DNA-binding"
                     /note="Myb-like DNA-binding domain; pfam00249"
                     /db_xref="CDD:459731"
     Region          <1417..1818
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1840..>1954
                     /region_name="MAP7"
                     /note="MAP7 (E-MAP-115) family; pfam05672"
                     /db_xref="CDD:461709"
     CDS             1..2388
                     /gene="Smr"
                     /coded_by="XM_076690572.1:845..8011"
                     /db_xref="GeneID:117611681"
ORIGIN      
        1 mqkrkdffet srlsvcmmkp tqnyvnikss spvsprtvgt avtyvqggaa assaaaaagt
       61 vgsgtaggps iggggggggs gggvgvsnas gansgtgvvg agsgaggavg nvgaagsgns
      121 sgtsnsaaga gsnsggnsgt vsgapggyva vpmagglryi hpypptptsa svpavatvvt
      181 sassaptpvp apaaapanpp tvsqtpppsl sgtaytarda yrsattnvne riaisvsssp
      241 lsqigvgvgv vtaeyaansg igsamaggrg drprisllpp asvtptpspt apdyqhvssv
      301 rnsriglmpv qpdqycsrta iemanlqeap pfkkirlgqp tqvqsqsqsq srcqslspad
      361 pcvkqehvvq lqqplridtr pttgaytpqt eaisptlpep ntqedaqfrs tkddllqqia
      421 kvdreiakre sqinklrkkl keleetankp leanglkrqv eeqsqqpkhq slaqkiyaen
      481 rrkaeeahrl lerlgpkvel plynqpsdts vyqenrtkhq tcmrarliar lrrehaeras
      541 lhrqqsqtya ilvqewhrkv erleatqkrk skeaknreff ekvfpelrkq redkerfnrv
      601 garikseadl eeimdglqeq emedkkmrsy avippllldt kqrriafqnr ngllqpeele
      661 alhserklin vwsavehelf kekylqhpkn fgtiaqsleh ksvpdcvhhy yltkkaenyk
      721 qllrksrqrt rssrnnpsnk eknnlppsig pidilstgvt trlqreqqqk tqenpqsnsa
      781 attttttttt tttssvstsa attivttaat alstptssic istesviast tsatttsvps
      841 ittcvttsat sattsakdnr etnkenketk seskeqhlvk vevkeepqwl nsetvagkdv
      901 kviidgksts ssssslsvna stlqsdlkds nkkktgcrea nsggsgtign asrikekkkd
      961 nhatpmetsd eeaqsidtie sgrqigphac avcqnivegn tqsralpprq asqyglredq
     1021 vapgarvcnt crckavrgry tacpllgcpn lnntssktkv krlralpakw fdlpaevrdp
     1081 iiqefqipnn atkccsacfn risrrlaphl tggtevpedt adslarqwtd eeleqlrral
     1141 rehgtnwvkv adqipgktnh qcknyyfayr kklsldqvva eyyqslgeer rpcltdeees
     1201 gsstsscdel avhdssdtas agspannmts gtpgtpgasa sanlpmpfsr stdqklgdkl
     1261 adiavvslvp pvsisssqqs stsggsnaas agtredydss atetadegqg stdlenssvv
     1321 vtlttpsngt plqqhqqshq qvpppshspp sttsnsnplt vkdlmlgvie mqlkrnpnsp
     1381 agtggssvpv nsgtptissi lrtdhrndit fvreykpsst mantsmprds nlatlsvvsg
     1441 phhghrsaps pqqigqiqat itpcvpapss sqsstsdilp keglvvmqvq qalretegtl
     1501 dlsikkprqq eynhslqtlh kpptvtlyrp epppgayyhp hshpeqgrga kspqvyassp
     1561 rpqapltpkm nkvavppphp klspklgamg ttghksgsit hgtpvssary egllrqmtpp
     1621 gnpgsvagtp svtsgpsavn apssqgpket gsitqgtpvh pfavdkrapm ydyhrtirps
     1681 pvttgnipgq gqsqagtaav vvthsnqyns ysgrpppsyt meqqlssrqi iindyitsqq
     1741 mharsrsgvt sesssknevp stlyytntpp pqthtqprqg viqrhnpqkq ihypppppgl
     1801 eafsslvdva vqqpslpvph phshpasgss sheglgktma drlladrygd khrlaihqdh
     1861 rmavahhhqr drererdlvh lrekeeyrlq rekeilqqdh rmaqqrekds qhadhrltvh
     1921 qqrekeihqq mavaaaqrek ehqehrlavq qqrerdlahl qqqqqqqqqq hlqqhiqqqr
     1981 meaerrhmvq lyrdqqqldr dsrlissgft qsrlqqpqqs qqqqqqqqqq qqsssrqsqs
     2041 sgqqqnesas tltaaslida iithqinqsv entggnsqpp rpgdrlfqgf hreasiepng
     2101 mahspagegn ggnngpgsas gngngnkpit lgehvdhivs kdygpphssy rsysgyqise
     2161 dqwkrrkane adsvkagder qiirvtqqqs qqqqqqqqqq qqqqqqhqhq hqqtassvgk
     2221 qqfhsvepvs ppeagtnhyt rrmyetttat stsgtpsnkp hlspldyvkn kivevmrtse
     2281 ddkggnagdr nggnvtsgdk dekiggnves pssgemmvde spnqtpqppa paptttfypf
     2341 salgvhtgpp papppptsgs tsvtkseqmq aepapllsaq yeplsded
//
LOCUS       XP_034195640             384 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid-4 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195640
VERSION     XP_034195640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339749.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="rhomboid-4 isoform X1"
                     /calculated_mol_wt=43518
     Region          44..108
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          202..346
                     /region_name="Rhomboid"
                     /note="Rhomboid family; pfam01694"
                     /db_xref="CDD:426384"
     CDS             1..384
                     /gene="rho-4"
                     /coded_by="XM_034339749.2:460..1614"
                     /db_xref="GeneID:117611683"
ORIGIN      
        1 mfspqrvmrq iseisyvggf klirmsttqd gesgitiplq ynnshwksif ekydldgdgk
       61 isyqelrami rssaysndip trvvhlimrk adlddsgyld ypefiamihr kdmqgvfghl
      121 vqryvqsmvp qrpstlhltr ssdfpdgqye eeytcnppal amiiisilei vlflydvivy
      181 rtpsvegpaa tlfiynphkr yqawryitym fvhvgvfhlv vnllvqimlg iplemvhkww
      241 rvliiylagv vagslgtsvs dprvylagas ggvyalitah vatilmnwsq mefavlqllv
      301 flvvtsadig qaiynryvla tddqvgyvah fagaiagllv ginvlrnlev ktwekvvwwa
      361 siitytalmt aaifwnilyt shye
//
LOCUS       XP_034195641             379 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid-4 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195641
VERSION     XP_034195641.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339750.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195641.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..379
                     /product="rhomboid-4 isoform X2"
                     /calculated_mol_wt=43127
     Region          39..103
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          197..341
                     /region_name="Rhomboid"
                     /note="Rhomboid family; pfam01694"
                     /db_xref="CDD:426384"
     CDS             1..379
                     /gene="rho-4"
                     /coded_by="XM_034339750.2:94..1233"
                     /db_xref="GeneID:117611683"
ORIGIN      
        1 mfirrtfrhs ckrgfklirm sttqdgesgi tiplqynnsh wksifekydl dgdgkisyqe
       61 lramirssay sndiptrvvh limrkadldd sgyldypefi amihrkdmqg vfghlvqryv
      121 qsmvpqrpst lhltrssdfp dgqyeeeytc nppalamiii sileivlfly dvivyrtpsv
      181 egpaatlfiy nphkryqawr yitymfvhvg vfhlvvnllv qimlgiplem vhkwwrvlii
      241 ylagvvagsl gtsvsdprvy lagasggvya litahvatil mnwsqmefav lqllvflvvt
      301 sadigqaiyn ryvlatddqv gyvahfagai agllvginvl rnlevktwek vvwwasiity
      361 talmtaaifw nilytshye
//
LOCUS       XP_034195643             360 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid-4 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195643
VERSION     XP_034195643.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339752.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="rhomboid-4 isoform X3"
                     /calculated_mol_wt=40692
     Region          20..84
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          178..322
                     /region_name="Rhomboid"
                     /note="Rhomboid family; pfam01694"
                     /db_xref="CDD:426384"
     CDS             1..360
                     /gene="rho-4"
                     /coded_by="XM_034339752.2:331..1413"
                     /db_xref="GeneID:117611683"
ORIGIN      
        1 msttqdgesg itiplqynns hwksifekyd ldgdgkisyq elramirssa ysndiptrvv
       61 hlimrkadld dsgyldypef iamihrkdmq gvfghlvqry vqsmvpqrps tlhltrssdf
      121 pdgqyeeeyt cnppalamii isileivlfl ydvivyrtps vegpaatlfi ynphkryqaw
      181 ryitymfvhv gvfhlvvnll vqimlgiple mvhkwwrvli iylagvvags lgtsvsdprv
      241 ylagasggvy alitahvati lmnwsqmefa vlqllvflvv tsadigqaiy nryvlatddq
      301 vgyvahfaga iagllvginv lrnlevktwe kvvwwasiit ytalmtaaif wnilytshye
//
LOCUS       XP_034195642             360 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid-4 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195642
VERSION     XP_034195642.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339751.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="rhomboid-4 isoform X3"
                     /calculated_mol_wt=40692
     Region          20..84
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          178..322
                     /region_name="Rhomboid"
                     /note="Rhomboid family; pfam01694"
                     /db_xref="CDD:426384"
     CDS             1..360
                     /gene="rho-4"
                     /coded_by="XM_034339751.2:534..1616"
                     /db_xref="GeneID:117611683"
ORIGIN      
        1 msttqdgesg itiplqynns hwksifekyd ldgdgkisyq elramirssa ysndiptrvv
       61 hlimrkadld dsgyldypef iamihrkdmq gvfghlvqry vqsmvpqrps tlhltrssdf
      121 pdgqyeeeyt cnppalamii isileivlfl ydvivyrtps vegpaatlfi ynphkryqaw
      181 ryitymfvhv gvfhlvvnll vqimlgiple mvhkwwrvli iylagvvags lgtsvsdprv
      241 ylagasggvy alitahvati lmnwsqmefa vlqllvflvv tsadigqaiy nryvlatddq
      301 vgyvahfaga iagllvginv lrnlevktwe kvvwwasiit ytalmtaaif wnilytshye
//
LOCUS       XP_034195644             360 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid-4 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195644
VERSION     XP_034195644.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339753.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="rhomboid-4 isoform X3"
                     /calculated_mol_wt=40692
     Region          20..84
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          178..322
                     /region_name="Rhomboid"
                     /note="Rhomboid family; pfam01694"
                     /db_xref="CDD:426384"
     CDS             1..360
                     /gene="rho-4"
                     /coded_by="XM_034339753.2:601..1683"
                     /db_xref="GeneID:117611683"
ORIGIN      
        1 msttqdgesg itiplqynns hwksifekyd ldgdgkisyq elramirssa ysndiptrvv
       61 hlimrkadld dsgyldypef iamihrkdmq gvfghlvqry vqsmvpqrps tlhltrssdf
      121 pdgqyeeeyt cnppalamii isileivlfl ydvivyrtps vegpaatlfi ynphkryqaw
      181 ryitymfvhv gvfhlvvnll vqimlgiple mvhkwwrvli iylagvvags lgtsvsdprv
      241 ylagasggvy alitahvati lmnwsqmefa vlqllvflvv tsadigqaiy nryvlatddq
      301 vgyvahfaga iagllvginv lrnlevktwe kvvwwasiit ytalmtaaif wnilytshye
//
LOCUS       XP_034195646             360 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid-4 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195646
VERSION     XP_034195646.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339755.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="rhomboid-4 isoform X3"
                     /calculated_mol_wt=40692
     Region          20..84
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          178..322
                     /region_name="Rhomboid"
                     /note="Rhomboid family; pfam01694"
                     /db_xref="CDD:426384"
     CDS             1..360
                     /gene="rho-4"
                     /coded_by="XM_034339755.2:162..1244"
                     /db_xref="GeneID:117611683"
ORIGIN      
        1 msttqdgesg itiplqynns hwksifekyd ldgdgkisyq elramirssa ysndiptrvv
       61 hlimrkadld dsgyldypef iamihrkdmq gvfghlvqry vqsmvpqrps tlhltrssdf
      121 pdgqyeeeyt cnppalamii isileivlfl ydvivyrtps vegpaatlfi ynphkryqaw
      181 ryitymfvhv gvfhlvvnll vqimlgiple mvhkwwrvli iylagvvags lgtsvsdprv
      241 ylagasggvy alitahvati lmnwsqmefa vlqllvflvv tsadigqaiy nryvlatddq
      301 vgyvahfaga iagllvginv lrnlevktwe kvvwwasiit ytalmtaaif wnilytshye
//
LOCUS       XP_034195647             360 aa            linear   INV 03-SEP-2025
DEFINITION  rhomboid-4 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195647
VERSION     XP_034195647.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339756.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="rhomboid-4 isoform X3"
                     /calculated_mol_wt=40692
     Region          20..84
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     Region          178..322
                     /region_name="Rhomboid"
                     /note="Rhomboid family; pfam01694"
                     /db_xref="CDD:426384"
     CDS             1..360
                     /gene="rho-4"
                     /coded_by="XM_034339756.2:159..1241"
                     /db_xref="GeneID:117611683"
ORIGIN      
        1 msttqdgesg itiplqynns hwksifekyd ldgdgkisyq elramirssa ysndiptrvv
       61 hlimrkadld dsgyldypef iamihrkdmq gvfghlvqry vqsmvpqrps tlhltrssdf
      121 pdgqyeeeyt cnppalamii isileivlfl ydvivyrtps vegpaatlfi ynphkryqaw
      181 ryitymfvhv gvfhlvvnll vqimlgiple mvhkwwrvli iylagvvags lgtsvsdprv
      241 ylagasggvy alitahvati lmnwsqmefa vlqllvflvv tsadigqaiy nryvlatddq
      301 vgyvahfaga iagllvginv lrnlevktwe kvvwwasiit ytalmtaaif wnilytshye
//
LOCUS       XP_034195639             508 aa            linear   INV 03-SEP-2025
DEFINITION  Meckel syndrome, type 1 [Osmia lignaria lignaria].
ACCESSION   XP_034195639
VERSION     XP_034195639.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339748.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195639.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="Meckel syndrome, type 1"
                     /calculated_mol_wt=59277
     Region          285..459
                     /region_name="B9-C2"
                     /note="Ciliary basal body-associated, B9 protein;
                     pfam07162"
                     /db_xref="CDD:462108"
     CDS             1..508
                     /gene="Mks1"
                     /coded_by="XM_034339748.2:56..1582"
                     /db_xref="GeneID:117611682"
ORIGIN      
        1 mpnkirkkki aanykvkepi gnfklrvkiv qqrplladll enegdtrdsn fleeedrifs
       61 wqekvfspfe atfysdqnnc lteqqklyyq qikdndiqgs llytytqnds yylenellte
      121 pcktrlgirn qtalpalqnr kpfqerynki vlddvpnktr irinhyhyme rstmyvmvdl
      181 sqrdktltss dddmetvlcv itydglhkil sinpdftddh cyiiansngv kfnywlehas
      241 ekpsslelqq qqnearreir nrlmykeaei shgiqltspd vykffikldi lsvhnfffdg
      301 lsvsyqmnlp eqwstnkedr lfgrtqrcnl innsayfsya temsldlhsa sietedtvpc
      361 wprlllsvis ldswsryrte gyaslplpvl pgsykfnipt wratgsiins lrrfftggty
      421 eleditycgi pqghegkmls ksqlnvtpsg niklnmnivh qtssakdrdq leylqhlstd
      481 kfmtnienif eqfkaarehm lkiknlni
//
LOCUS       XP_034195648             312 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein L44 [Osmia lignaria lignaria].
ACCESSION   XP_034195648
VERSION     XP_034195648.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339757.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="mitochondrial ribosomal protein L44"
                     /calculated_mol_wt=35152
     Region          77..209
                     /region_name="RIBOc"
                     /note="Ribonuclease III C terminal domain. This group
                     consists of eukaryotic, bacterial and archeal ribonuclease
                     III (RNAse III) proteins. RNAse III is a double stranded
                     RNA-specific endonuclease. Prokaryotic RNAse III is
                     important in post-transcriptional...; cl00258"
                     /db_xref="CDD:469693"
     Region          213..296
                     /region_name="DSRM_MRPL44"
                     /note="double-stranded RNA binding motif of mitochondrial
                     39S ribosomal protein L44 (MRPL44) and similar proteins;
                     cd19874"
                     /db_xref="CDD:380703"
     Site            order(213..214,216,219,225,227..228,230..231,235,242..244,
                     251..252,254..257,259,263,276..279,282,286)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380703"
     CDS             1..312
                     /gene="mRpL44"
                     /coded_by="XM_034339757.2:80..1018"
                     /db_xref="GeneID:117611684"
ORIGIN      
        1 mnklrlclts iatlnainyg gcrhfkrwva ptlkeickrk eqlppqpepk rstfiewnre
       61 aelyafnqrl kekfdmekln qafihksyil qelkkqeemg itdpkldiqd nreliekgke
      121 itseivtiyl nmalpdlpes gfvslydhim sqeilakasl higtkdiilt aehpaseetl
      181 aqtflalvga ltestnkghv sdfvqdflit gladkdltei wtpakpfeml sdimsterka
      241 sieariigqa gtntilsayh vgiyidrefl gsgfgqtiee aknvaatnil samygisess
      301 qplpftnkyf fi
//
LOCUS       XP_034195650             224 aa            linear   INV 03-SEP-2025
DEFINITION  ER membrane protein complex subunit 7 [Osmia lignaria lignaria].
ACCESSION   XP_034195650
VERSION     XP_034195650.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339759.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195650.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..224
                     /product="ER membrane protein complex subunit 7"
                     /calculated_mol_wt=25869
     Region          48..159
                     /region_name="EMC7_beta-sandw"
                     /note="ER membrane protein complex subunit 7,
                     beta-sandwich domain; pfam09430"
                     /db_xref="CDD:430607"
     CDS             1..224
                     /gene="EMC7"
                     /coded_by="XM_034339759.2:76..750"
                     /db_xref="GeneID:117611685"
ORIGIN      
        1 mimhrsvifl fflivnisnk yviaendedt stelyiiegk vfpwengasn gwqlmthvma
       61 nggehygflr edgtfiisnv psgsyiievv npncvyepvr veinskgkfr arkvnliqta
      121 qvtqvpyplk mrpltpfryf qvreqwrvtd flfnpmvlmm ilpfllimii pkimndpetr
      181 kemeqlnnft nynipemsev itsyclsgge kqktkavkaa kkrq
//
LOCUS       XP_034195653             218 aa            linear   INV 03-SEP-2025
DEFINITION  vacuolar protein sorting 37B [Osmia lignaria lignaria].
ACCESSION   XP_034195653
VERSION     XP_034195653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339762.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..218
                     /product="vacuolar protein sorting 37B"
                     /calculated_mol_wt=24415
     Region          18..162
                     /region_name="Mod_r"
                     /note="Modifier of rudimentary (Mod(r)) protein;
                     pfam07200"
                     /db_xref="CDD:462117"
     CDS             1..218
                     /gene="Vps37B"
                     /coded_by="XM_034339762.2:371..1027"
                     /db_xref="GeneID:117611687"
ORIGIN      
        1 mfqsnqepdi paalgllshl tndelkelln ndgkfedivk dikqfkdlet ekemlmasnr
       61 slaefnlskq pelqdgkqil relsekgtnl ctsvkdklde ireksgemtv ntaldllqta
      121 ateieeeset iaekflagdm evdefleqfl srrklmhlrk vkvdklreli rkshsvtsgp
      181 gypiasnfpg iapsvpyptg pvsmpmpvps glphyrpy
//
LOCUS       XP_034195655             600 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611688 [Osmia lignaria lignaria].
ACCESSION   XP_034195655
VERSION     XP_034195655.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339764.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195655.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..600
                     /product="uncharacterized protein LOC117611688"
                     /calculated_mol_wt=65364
     Region          17..154
                     /region_name="BTB"
                     /note="BTB/POZ domain; pfam00651"
                     /db_xref="CDD:395526"
     Region          <316..390
                     /region_name="DMP1"
                     /note="Dentin matrix protein 1 (DMP1); pfam07263"
                     /db_xref="CDD:462128"
     Region          492..514
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     CDS             1..600
                     /gene="LOC117611688"
                     /coded_by="XM_034339764.2:78..1880"
                     /db_xref="GeneID:117611688"
ORIGIN      
        1 myslyrawfg dsvnadnysr iqetlsppdt vvrvgnegeh qfiahkgvla ahsgylkall
       61 tnatntpstv asscsnlpat aiavtatnls eqsmrqpvts vsvssiggea fapllnymyt
      121 grlevtldnv ysvllathll hmpgaleqcr aallrlrapp plptpmlvpt gptstltsts
      181 mststgsgni lrpipnrlmi dpsmcwppat plypptapap agiphmpqlq psvlmqstvp
      241 itvvptssvq etqsyrevps pksslfqmer nrglrmdnra kspetttsts lplvaaaaaa
      301 ftafttsnaa tstrtsspsq sisptlsses qsplpvcqsk tseqrqikee teyeqrnsng
      361 gqtqsrsspv tsveetsstr drsvesgend rqrssrrrgr gstsrndgts sgvlsvvydv
      421 accdgpvkfh rvlnknysss gsnpalqprl qrscfepenv stendenggp aevrpcdpir
      481 sdidtantsg sytcgycrht fksqycyrkh tkrhllptrv netvvnrprq dtarsrrevr
      541 lldlnvqyyp ckicgckfps yyfvhkhrkl chanaeerpq eeastaaedl asttssnerq
//
LOCUS       XP_034195429             774 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034195429
VERSION     XP_034195429.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339538.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..774
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X1"
                     /calculated_mol_wt=83037
     Region          24..134
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <485..772
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          489..530
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          538..576
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          581..617
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          624..659
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          664..697
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          705..739
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          746..770
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..774
                     /gene="gro"
                     /coded_by="XM_034339538.2:788..3112"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmv rhfyyemsyg lnvemhkqte iakrlnaiiv qvlpyltqeh glqhqqqvan
      121 averakqvtm telnaiigqq qqqglqqllq qihaqqlphg avvgglppag llgfagaaaa
      181 aaaagvpphl appphpaaaa avqaqvqall kpsdlqqhra aaetpedrkp ialnderlrr
      241 svsppekfrs rtpdlesdpk rrkeeklghd sdgeksdqdl vvdvaneeaa sphangehsd
      301 prengtlekl gapghvgpts gagstsvkdr ppsrsgsssa rstpslkskd idkpgtpvaa
      361 argsrsctpt mggipgplas rvyhppssqq tppqgyqglp srgneppqsp spysnlpyar
      421 tsmlgfdghv ripssnglsn ipggkpaysf hmngegqlqp vpfptdalig pgiprharqi
      481 ntlthgevvc avtisnptky vytggkgcvk vwdighgggg sikhvsqldc lqrdnyirsv
      541 kllpdcrtli vggeasqlii wdlasptpri kaeltssapa cyalaispds kvcfsccsdg
      601 niavwdlqne ilvrqfqght dgascidisa dgsklwtggl dntvrswdlr egrqlqqhdf
      661 tsqifslgyc ptgewlavgm ensnvevlha sksdkyqlhl hescvlslrf ascgkwfvst
      721 gkdnllnawr tpygasifqs kesssvlscd isaddkyivt gsgdkkatvy eviy
//
LOCUS       XP_034195430             774 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034195430
VERSION     XP_034195430.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339539.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..774
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X1"
                     /calculated_mol_wt=83037
     Region          24..134
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <485..772
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          489..530
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          538..576
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          581..617
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          624..659
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          664..697
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          705..739
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          746..770
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..774
                     /gene="gro"
                     /coded_by="XM_034339539.2:307..2631"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmv rhfyyemsyg lnvemhkqte iakrlnaiiv qvlpyltqeh glqhqqqvan
      121 averakqvtm telnaiigqq qqqglqqllq qihaqqlphg avvgglppag llgfagaaaa
      181 aaaagvpphl appphpaaaa avqaqvqall kpsdlqqhra aaetpedrkp ialnderlrr
      241 svsppekfrs rtpdlesdpk rrkeeklghd sdgeksdqdl vvdvaneeaa sphangehsd
      301 prengtlekl gapghvgpts gagstsvkdr ppsrsgsssa rstpslkskd idkpgtpvaa
      361 argsrsctpt mggipgplas rvyhppssqq tppqgyqglp srgneppqsp spysnlpyar
      421 tsmlgfdghv ripssnglsn ipggkpaysf hmngegqlqp vpfptdalig pgiprharqi
      481 ntlthgevvc avtisnptky vytggkgcvk vwdighgggg sikhvsqldc lqrdnyirsv
      541 kllpdcrtli vggeasqlii wdlasptpri kaeltssapa cyalaispds kvcfsccsdg
      601 niavwdlqne ilvrqfqght dgascidisa dgsklwtggl dntvrswdlr egrqlqqhdf
      661 tsqifslgyc ptgewlavgm ensnvevlha sksdkyqlhl hescvlslrf ascgkwfvst
      721 gkdnllnawr tpygasifqs kesssvlscd isaddkyivt gsgdkkatvy eviy
//
LOCUS       XP_034195431             774 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034195431
VERSION     XP_034195431.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339540.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..774
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X1"
                     /calculated_mol_wt=83037
     Region          24..134
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <485..772
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          489..530
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          538..576
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          581..617
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          624..659
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          664..697
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          705..739
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          746..770
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..774
                     /gene="gro"
                     /coded_by="XM_034339540.2:325..2649"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmv rhfyyemsyg lnvemhkqte iakrlnaiiv qvlpyltqeh glqhqqqvan
      121 averakqvtm telnaiigqq qqqglqqllq qihaqqlphg avvgglppag llgfagaaaa
      181 aaaagvpphl appphpaaaa avqaqvqall kpsdlqqhra aaetpedrkp ialnderlrr
      241 svsppekfrs rtpdlesdpk rrkeeklghd sdgeksdqdl vvdvaneeaa sphangehsd
      301 prengtlekl gapghvgpts gagstsvkdr ppsrsgsssa rstpslkskd idkpgtpvaa
      361 argsrsctpt mggipgplas rvyhppssqq tppqgyqglp srgneppqsp spysnlpyar
      421 tsmlgfdghv ripssnglsn ipggkpaysf hmngegqlqp vpfptdalig pgiprharqi
      481 ntlthgevvc avtisnptky vytggkgcvk vwdighgggg sikhvsqldc lqrdnyirsv
      541 kllpdcrtli vggeasqlii wdlasptpri kaeltssapa cyalaispds kvcfsccsdg
      601 niavwdlqne ilvrqfqght dgascidisa dgsklwtggl dntvrswdlr egrqlqqhdf
      661 tsqifslgyc ptgewlavgm ensnvevlha sksdkyqlhl hescvlslrf ascgkwfvst
      721 gkdnllnawr tpygasifqs kesssvlscd isaddkyivt gsgdkkatvy eviy
//
LOCUS       XP_034195432             770 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034195432
VERSION     XP_034195432.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339541.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..770
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X2"
                     /calculated_mol_wt=82497
     Region          24..130
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <481..768
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          485..526
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          534..572
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          577..613
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          620..655
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          660..693
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          701..735
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          742..766
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..770
                     /gene="gro"
                     /coded_by="XM_034339541.2:784..3096"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmy yemsyglnve mhkqteiakr lnaiivqvlp yltqehglqh qqqvanaver
      121 akqvtmteln aiigqqqqqg lqqllqqiha qqlphgavvg glppagllgf agaaaaaaaa
      181 gvpphlappp hpaaaaavqa qvqallkpsd lqqhraaaet pedrkpialn derlrrsvsp
      241 pekfrsrtpd lesdpkrrke eklghdsdge ksdqdlvvdv aneeaaspha ngehsdpren
      301 gtleklgapg hvgptsgags tsvkdrppsr sgsssarstp slkskdidkp gtpvaaargs
      361 rsctptmggi pgplasrvyh ppssqqtppq gyqglpsrgn eppqspspys nlpyartsml
      421 gfdghvrips snglsnipgg kpaysfhmng egqlqpvpfp tdaligpgip rharqintlt
      481 hgevvcavti snptkyvytg gkgcvkvwdi ghggggsikh vsqldclqrd nyirsvkllp
      541 dcrtlivgge asqliiwdla sptprikael tssapacyal aispdskvcf sccsdgniav
      601 wdlqneilvr qfqghtdgas cidisadgsk lwtggldntv rswdlregrq lqqhdftsqi
      661 fslgycptge wlavgmensn vevlhasksd kyqlhlhesc vlslrfascg kwfvstgkdn
      721 llnawrtpyg asifqskess svlscdisad dkyivtgsgd kkatvyeviy
//
LOCUS       XP_034195433             770 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034195433
VERSION     XP_034195433.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339542.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..770
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X3"
                     /calculated_mol_wt=82601
     Region          24..130
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <481..768
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          485..526
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          534..572
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          577..613
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          620..655
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          660..693
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          701..735
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          742..766
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..770
                     /gene="gro"
                     /coded_by="XM_034339542.2:786..3098"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmv rhfyyemsyg lnvemhkqte iakrlnaiiv qvlpyltqeh qqqvanaver
      121 akqvtmteln aiigqqqqqg lqqllqqiha qqlphgavvg glppagllgf agaaaaaaaa
      181 gvpphlappp hpaaaaavqa qvqallkpsd lqqhraaaet pedrkpialn derlrrsvsp
      241 pekfrsrtpd lesdpkrrke eklghdsdge ksdqdlvvdv aneeaaspha ngehsdpren
      301 gtleklgapg hvgptsgags tsvkdrppsr sgsssarstp slkskdidkp gtpvaaargs
      361 rsctptmggi pgplasrvyh ppssqqtppq gyqglpsrgn eppqspspys nlpyartsml
      421 gfdghvrips snglsnipgg kpaysfhmng egqlqpvpfp tdaligpgip rharqintlt
      481 hgevvcavti snptkyvytg gkgcvkvwdi ghggggsikh vsqldclqrd nyirsvkllp
      541 dcrtlivgge asqliiwdla sptprikael tssapacyal aispdskvcf sccsdgniav
      601 wdlqneilvr qfqghtdgas cidisadgsk lwtggldntv rswdlregrq lqqhdftsqi
      661 fslgycptge wlavgmensn vevlhasksd kyqlhlhesc vlslrfascg kwfvstgkdn
      721 llnawrtpyg asifqskess svlscdisad dkyivtgsgd kkatvyeviy
//
LOCUS       XP_034195434             766 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034195434
VERSION     XP_034195434.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339543.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..766
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X4"
                     /calculated_mol_wt=82062
     Region          24..126
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <477..764
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          481..522
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          530..568
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          573..609
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          616..651
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          656..689
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          697..731
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          738..762
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..766
                     /gene="gro"
                     /coded_by="XM_034339543.2:782..3082"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmy yemsyglnve mhkqteiakr lnaiivqvlp yltqehqqqv anaverakqv
      121 tmtelnaiig qqqqqglqql lqqihaqqlp hgavvgglpp agllgfagaa aaaaaagvpp
      181 hlappphpaa aaavqaqvqa llkpsdlqqh raaaetpedr kpialnderl rrsvsppekf
      241 rsrtpdlesd pkrrkeeklg hdsdgeksdq dlvvdvanee aasphangeh sdprengtle
      301 klgapghvgp tsgagstsvk drppsrsgss sarstpslks kdidkpgtpv aaargsrsct
      361 ptmggipgpl asrvyhppss qqtppqgyqg lpsrgneppq spspysnlpy artsmlgfdg
      421 hvripssngl snipggkpay sfhmngegql qpvpfptdal igpgiprhar qintlthgev
      481 vcavtisnpt kyvytggkgc vkvwdighgg ggsikhvsql dclqrdnyir svkllpdcrt
      541 livggeasql iiwdlasptp rikaeltssa pacyalaisp dskvcfsccs dgniavwdlq
      601 neilvrqfqg htdgascidi sadgsklwtg gldntvrswd lregrqlqqh dftsqifslg
      661 ycptgewlav gmensnvevl hasksdkyql hlhescvlsl rfascgkwfv stgkdnllna
      721 wrtpygasif qskesssvls cdisaddkyi vtgsgdkkat vyeviy
//
LOCUS       XP_034195435             712 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034195435
VERSION     XP_034195435.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339544.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..712
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X5"
                     /calculated_mol_wt=76479
     Region          <1..72
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <423..710
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          427..468
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          476..514
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          519..555
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          562..597
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          602..635
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          643..677
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          684..708
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..712
                     /gene="gro"
                     /coded_by="XM_034339544.2:867..3005"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mqrhyvmvrh fyyemsygln vemhkqteia krlnaiivqv lpyltqehgl qhqqqvanav
       61 erakqvtmte lnaiigqqqq qglqqllqqi haqqlphgav vgglppagll gfagaaaaaa
      121 aagvpphlap pphpaaaaav qaqvqallkp sdlqqhraaa etpedrkpia lnderlrrsv
      181 sppekfrsrt pdlesdpkrr keeklghdsd geksdqdlvv dvaneeaasp hangehsdpr
      241 engtleklga pghvgptsga gstsvkdrpp srsgsssars tpslkskdid kpgtpvaaar
      301 gsrsctptmg gipgplasrv yhppssqqtp pqgyqglpsr gneppqspsp ysnlpyarts
      361 mlgfdghvri pssnglsnip ggkpaysfhm ngegqlqpvp fptdaligpg iprharqint
      421 lthgevvcav tisnptkyvy tggkgcvkvw dighggggsi khvsqldclq rdnyirsvkl
      481 lpdcrtlivg geasqliiwd lasptprika eltssapacy alaispdskv cfsccsdgni
      541 avwdlqneil vrqfqghtdg ascidisadg sklwtggldn tvrswdlreg rqlqqhdfts
      601 qifslgycpt gewlavgmen snvevlhask sdkyqlhlhe scvlslrfas cgkwfvstgk
      661 dnllnawrtp ygasifqske sssvlscdis addkyivtgs gdkkatvyev iy
//
LOCUS       XP_034195436             711 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X6
            [Osmia lignaria lignaria].
ACCESSION   XP_034195436
VERSION     XP_034195436.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339545.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..711
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X6"
                     /calculated_mol_wt=76761
     Region          24..134
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <422..709
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          426..467
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          475..513
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          518..554
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          561..596
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          601..634
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          642..676
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          683..707
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..711
                     /gene="gro"
                     /coded_by="XM_034339545.2:790..2925"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmv rhfyyemsyg lnvemhkqte iakrlnaiiv qvlpyltqeh glqhqqqvan
      121 averakqvtm telnaiigqq qqqglqqllq qihaqqlphg avvgglppag llgfagaaaa
      181 aaaagvpphl appphpaaaa avqaqvqall kpsdlqqhra aaetpedrkp ialnderlrr
      241 svsppekfrs rtpdlesdpk rrkeeklghd sdgeksdqdl vvdvaneidk pgtpvaaarg
      301 srsctptmgg ipgplasrvy hppssqqtpp qgyqglpsrg neppqspspy snlpyartsm
      361 lgfdghvrip ssnglsnipg gkpaysfhmn gegqlqpvpf ptdaligpgi prharqintl
      421 thgevvcavt isnptkyvyt ggkgcvkvwd ighggggsik hvsqldclqr dnyirsvkll
      481 pdcrtlivgg easqliiwdl asptprikae ltssapacya laispdskvc fsccsdgnia
      541 vwdlqneilv rqfqghtdga scidisadgs klwtggldnt vrswdlregr qlqqhdftsq
      601 ifslgycptg ewlavgmens nvevlhasks dkyqlhlhes cvlslrfasc gkwfvstgkd
      661 nllnawrtpy gasifqskes ssvlscdisa ddkyivtgsg dkkatvyevi y
//
LOCUS       XP_034195437             708 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X7
            [Osmia lignaria lignaria].
ACCESSION   XP_034195437
VERSION     XP_034195437.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339546.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X7"
                     /calculated_mol_wt=75940
     Region          <1..68
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <419..706
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          423..464
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          472..510
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          515..551
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          558..593
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          598..631
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          639..673
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          680..704
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..708
                     /gene="gro"
                     /coded_by="XM_034339546.2:866..2992"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mqrhyvmyye msyglnvemh kqteiakrln aiivqvlpyl tqehglqhqq qvanaverak
       61 qvtmtelnai igqqqqqglq qllqqihaqq lphgavvggl ppagllgfag aaaaaaaagv
      121 pphlappphp aaaaavqaqv qallkpsdlq qhraaaetpe drkpialnde rlrrsvsppe
      181 kfrsrtpdle sdpkrrkeek lghdsdgeks dqdlvvdvan eeaasphang ehsdprengt
      241 leklgapghv gptsgagsts vkdrppsrsg sssarstpsl kskdidkpgt pvaaargsrs
      301 ctptmggipg plasrvyhpp ssqqtppqgy qglpsrgnep pqspspysnl pyartsmlgf
      361 dghvripssn glsnipggkp aysfhmngeg qlqpvpfptd aligpgiprh arqintlthg
      421 evvcavtisn ptkyvytggk gcvkvwdigh ggggsikhvs qldclqrdny irsvkllpdc
      481 rtlivggeas qliiwdlasp tprikaelts sapacyalai spdskvcfsc csdgniavwd
      541 lqneilvrqf qghtdgasci disadgsklw tggldntvrs wdlregrqlq qhdftsqifs
      601 lgycptgewl avgmensnve vlhasksdky qlhlhescvl slrfascgkw fvstgkdnll
      661 nawrtpygas ifqskesssv lscdisaddk yivtgsgdkk atvyeviy
//
LOCUS       XP_034195438             701 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X8
            [Osmia lignaria lignaria].
ACCESSION   XP_034195438
VERSION     XP_034195438.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339547.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..701
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X8"
                     /calculated_mol_wt=75450
     Region          24..134
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <412..699
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          416..457
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          465..503
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          508..544
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          551..586
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          591..624
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          632..666
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          673..697
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..701
                     /gene="gro"
                     /coded_by="XM_034339547.2:794..2899"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmv rhfyyemsyg lnvemhkqte iakrlnaiiv qvlpyltqeh glqhqqqvan
      121 averakqvtm telnaiigqq qqqglqqllq qihaqqlphg avvgglppag llgfagaaaa
      181 aaaagvpphl appphpaaaa avqaqvqall kpsdlqqhra aaetpedrkp ialnderlrr
      241 svsppekfrs rtpdlesdpk rrkeeklghd sdgeksdqdl vvdvaneeaa sphangehsd
      301 prengtlekl gapghvgpts gagstsvkdr ppsrsgsssa rstpslkskd lgfdghvrip
      361 ssnglsnipg gkpaysfhmn gegqlqpvpf ptdaligpgi prharqintl thgevvcavt
      421 isnptkyvyt ggkgcvkvwd ighggggsik hvsqldclqr dnyirsvkll pdcrtlivgg
      481 easqliiwdl asptprikae ltssapacya laispdskvc fsccsdgnia vwdlqneilv
      541 rqfqghtdga scidisadgs klwtggldnt vrswdlregr qlqqhdftsq ifslgycptg
      601 ewlavgmens nvevlhasks dkyqlhlhes cvlslrfasc gkwfvstgkd nllnawrtpy
      661 gasifqskes ssvlscdisa ddkyivtgsg dkkatvyevi y
//
LOCUS       XP_034195439             638 aa            linear   INV 03-SEP-2025
DEFINITION  TLE family member transcriptional corepressor groucho isoform X9
            [Osmia lignaria lignaria].
ACCESSION   XP_034195439
VERSION     XP_034195439.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339548.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..638
                     /product="TLE family member transcriptional corepressor
                     groucho isoform X9"
                     /calculated_mol_wt=69174
     Region          24..134
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <349..636
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          353..394
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          402..440
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          445..481
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          488..523
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          528..561
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          569..603
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          610..634
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..638
                     /gene="gro"
                     /coded_by="XM_034339548.2:792..2708"
                     /db_xref="GeneID:117611573"
ORIGIN      
        1 mypaparhpa apggggsgga agglkftvad tcerikeefn fiqqqnhslk ieceklasek
       61 temqrhyvmv rhfyyemsyg lnvemhkqte iakrlnaiiv qvlpyltqeh glqhqqqvan
      121 averakqvtm telnaiigqq qqqglqqllq qihaqqlphg avvgglppag llgfagaaaa
      181 aaaagvpphl appphpaaaa avqaqvqall kpsdlqqhra aaetpedrkp ialnderlrr
      241 svsppekfrs rtpdlesdpk rrkeeklghd sdgeksdqdl vvdvanelgf dghvripssn
      301 glsnipggkp aysfhmngeg qlqpvpfptd aligpgiprh arqintlthg evvcavtisn
      361 ptkyvytggk gcvkvwdigh ggggsikhvs qldclqrdny irsvkllpdc rtlivggeas
      421 qliiwdlasp tprikaelts sapacyalai spdskvcfsc csdgniavwd lqneilvrqf
      481 qghtdgasci disadgsklw tggldntvrs wdlregrqlq qhdftsqifs lgycptgewl
      541 avgmensnve vlhasksdky qlhlhescvl slrfascgkw fvstgkdnll nawrtpygas
      601 ifqskesssv lscdisaddk yivtgsgdkk atvyeviy
//
LOCUS       XP_034195440             623 aa            linear   INV 03-SEP-2025
DEFINITION  protein fem-1 homolog CG6966 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195440
VERSION     XP_034195440.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339549.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..623
                     /product="protein fem-1 homolog CG6966 isoform X1"
                     /calculated_mol_wt=69278
     Region          10..112
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          40..72
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <82..273
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          82..113
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          118..146
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(121..122,125..127,129..130,134,137,146,148,150,
                     154..155,158..160,162..163,167,170,179,181,183,187..188,
                     191..193,195..196,200,203)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          148..179
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          181..210
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <420..>573
                     /region_name="PHA02876"
                     /note="ankyrin repeat protein; Provisional"
                     /db_xref="CDD:165207"
     Region          <517..587
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          535..568
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..623
                     /gene="LOC117611575"
                     /coded_by="XM_034339549.2:393..2264"
                     /db_xref="GeneID:117611575"
ORIGIN      
        1 mdfksvvyna ardgtlkrlk vfldhrgkde vgqlvgakth gatplvmacr nghydvaeyl
       61 ikecgadieq pgsvvfgget iegapplwca aaaghlalvr llvkrgakvn sttktnstpl
      121 raacfdgyfe ivrflvnhga diemanhqgh tslmiacykg hikiakflla lkadvnrksv
      181 rgytalhdca esgsldivkl liehgarmdv dsygmtpllv aavtghkhiv eyfigipnlv
      241 srkeridale llgatyvdke rdmigalefw kramderyrr dspviakplp ppvvaaydft
      301 reisnpdeld dlltdpdemr mqalvireri lgpahpdtsy yiryrgalya dggkfdrcik
      361 lwnyaldmqq smlepldpmt qsslfsftel fsfmigtqts tgrrvppvqk kellrvfkka
      421 vlevalgkqm ldkipvckrd ltylnrvlvi tlhlaclltr eipekgtdey itlhkaiyel
      481 vginakgkqg rdvlqliyse kgavvgsyst skfpssnlan alikvgadvt ardndgntal
      541 hlaavshfrr pdlavtllea gahidtvnne gktfemllrd krlydsvcpv kyttltclaa
      601 rvvkrtyele dvpkhlrafv qmh
//
LOCUS       XP_034195441             615 aa            linear   INV 03-SEP-2025
DEFINITION  protein fem-1 homolog CG6966 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195441
VERSION     XP_034195441.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339550.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..615
                     /product="protein fem-1 homolog CG6966 isoform X2"
                     /calculated_mol_wt=68520
     Region          <4..60
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          32..64
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <33..104
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <74..265
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          74..105
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          110..138
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(113..114,117..119,121..122,126,129,138,140,142,
                     146..147,150..152,154..155,159,162,171,173,175,179..180,
                     183..185,187..188,192,195)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          140..171
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          173..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <412..>565
                     /region_name="PHA02876"
                     /note="ankyrin repeat protein; Provisional"
                     /db_xref="CDD:165207"
     Region          <509..579
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          527..560
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..615
                     /gene="LOC117611575"
                     /coded_by="XM_034339550.2:2105..3952"
                     /db_xref="GeneID:117611575"
ORIGIN      
        1 meyeaneehf trvfldhrgk devgqlvgak thgatplvma crnghydvae ylikecgadi
       61 eqpgsvvfgg etiegapplw caaaaghlal vrllvkrgak vnsttktnst plraacfdgy
      121 feivrflvnh gadiemanhq ghtslmiacy kghikiakfl lalkadvnrk svrgytalhd
      181 caesgsldiv klliehgarm dvdsygmtpl lvaavtghkh iveyfigipn lvsrkerida
      241 lellgatyvd kerdmigale fwkramdery rrdspviakp lpppvvaayd ftreisnpde
      301 lddlltdpde mrmqalvire rilgpahpdt syyiryrgal yadggkfdrc iklwnyaldm
      361 qqsmlepldp mtqsslfsft elfsfmigtq tstgrrvppv qkkellrvfk kavlevalgk
      421 qmldkipvck rdltylnrvl vitlhlacll treipekgtd eyitlhkaiy elvginakgk
      481 qgrdvlqliy sekgavvgsy stskfpssnl analikvgad vtardndgnt alhlaavshf
      541 rrpdlavtll eagahidtvn negktfemll rdkrlydsvc pvkyttltcl aarvvkrtye
      601 ledvpkhlra fvqmh
//
LOCUS       XP_034195443             615 aa            linear   INV 03-SEP-2025
DEFINITION  protein fem-1 homolog CG6966 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195443
VERSION     XP_034195443.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339552.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..615
                     /product="protein fem-1 homolog CG6966 isoform X2"
                     /calculated_mol_wt=68520
     Region          <4..60
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          32..64
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <33..104
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <74..265
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          74..105
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          110..138
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(113..114,117..119,121..122,126,129,138,140,142,
                     146..147,150..152,154..155,159,162,171,173,175,179..180,
                     183..185,187..188,192,195)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          140..171
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          173..202
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <412..>565
                     /region_name="PHA02876"
                     /note="ankyrin repeat protein; Provisional"
                     /db_xref="CDD:165207"
     Region          <509..579
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          527..560
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..615
                     /gene="LOC117611575"
                     /coded_by="XM_034339552.2:1986..3833"
                     /db_xref="GeneID:117611575"
ORIGIN      
        1 meyeaneehf trvfldhrgk devgqlvgak thgatplvma crnghydvae ylikecgadi
       61 eqpgsvvfgg etiegapplw caaaaghlal vrllvkrgak vnsttktnst plraacfdgy
      121 feivrflvnh gadiemanhq ghtslmiacy kghikiakfl lalkadvnrk svrgytalhd
      181 caesgsldiv klliehgarm dvdsygmtpl lvaavtghkh iveyfigipn lvsrkerida
      241 lellgatyvd kerdmigale fwkramdery rrdspviakp lpppvvaayd ftreisnpde
      301 lddlltdpde mrmqalvire rilgpahpdt syyiryrgal yadggkfdrc iklwnyaldm
      361 qqsmlepldp mtqsslfsft elfsfmigtq tstgrrvppv qkkellrvfk kavlevalgk
      421 qmldkipvck rdltylnrvl vitlhlacll treipekgtd eyitlhkaiy elvginakgk
      481 qgrdvlqliy sekgavvgsy stskfpssnl analikvgad vtardndgnt alhlaavshf
      541 rrpdlavtll eagahidtvn negktfemll rdkrlydsvc pvkyttltcl aarvvkrtye
      601 ledvpkhlra fvqmh
//
LOCUS       XP_034195444             577 aa            linear   INV 03-SEP-2025
DEFINITION  protein fem-1 homolog CG6966 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195444
VERSION     XP_034195444.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339553.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="protein fem-1 homolog CG6966 isoform X3"
                     /calculated_mol_wt=64256
     Region          <1..66
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <36..227
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          36..67
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          72..100
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(75..76,79..81,83..84,88,91,100,102,104,108..109,
                     112..114,116..117,121,124,133,135,137,141..142,145..147,
                     149..150,154,157)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          102..133
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          135..164
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <374..>527
                     /region_name="PHA02876"
                     /note="ankyrin repeat protein; Provisional"
                     /db_xref="CDD:165207"
     Region          <471..541
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          489..522
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..577
                     /gene="LOC117611575"
                     /coded_by="XM_034339553.2:1997..3730"
                     /db_xref="GeneID:117611575"
ORIGIN      
        1 macrnghydv aeylikecga dieqpgsvvf ggetiegapp lwcaaaaghl alvrllvkrg
       61 akvnsttktn stplraacfd gyfeivrflv nhgadieman hqghtslmia cykghikiak
      121 fllalkadvn rksvrgytal hdcaesgsld ivklliehga rmdvdsygmt pllvaavtgh
      181 khiveyfigi pnlvsrkeri dalellgaty vdkerdmiga lefwkramde ryrrdspvia
      241 kplpppvvaa ydftreisnp delddlltdp demrmqalvi rerilgpahp dtsyyiryrg
      301 alyadggkfd rciklwnyal dmqqsmlepl dpmtqsslfs ftelfsfmig tqtstgrrvp
      361 pvqkkellrv fkkavleval gkqmldkipv ckrdltylnr vlvitlhlac lltreipekg
      421 tdeyitlhka iyelvginak gkqgrdvlql iysekgavvg systskfpss nlanalikvg
      481 advtardndg ntalhlaavs hfrrpdlavt lleagahidt vnnegktfem llrdkrlyds
      541 vcpvkyttlt claarvvkrt yeledvpkhl rafvqmh
//
LOCUS       XP_034195445             577 aa            linear   INV 03-SEP-2025
DEFINITION  protein fem-1 homolog CG6966 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195445
VERSION     XP_034195445.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339554.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="protein fem-1 homolog CG6966 isoform X3"
                     /calculated_mol_wt=64256
     Region          <1..66
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          <36..227
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          36..67
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          72..100
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(75..76,79..81,83..84,88,91,100,102,104,108..109,
                     112..114,116..117,121,124,133,135,137,141..142,145..147,
                     149..150,154,157)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          102..133
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          135..164
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          <374..>527
                     /region_name="PHA02876"
                     /note="ankyrin repeat protein; Provisional"
                     /db_xref="CDD:165207"
     Region          <471..541
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          489..522
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..577
                     /gene="LOC117611575"
                     /coded_by="XM_034339554.2:252..1985"
                     /db_xref="GeneID:117611575"
ORIGIN      
        1 macrnghydv aeylikecga dieqpgsvvf ggetiegapp lwcaaaaghl alvrllvkrg
       61 akvnsttktn stplraacfd gyfeivrflv nhgadieman hqghtslmia cykghikiak
      121 fllalkadvn rksvrgytal hdcaesgsld ivklliehga rmdvdsygmt pllvaavtgh
      181 khiveyfigi pnlvsrkeri dalellgaty vdkerdmiga lefwkramde ryrrdspvia
      241 kplpppvvaa ydftreisnp delddlltdp demrmqalvi rerilgpahp dtsyyiryrg
      301 alyadggkfd rciklwnyal dmqqsmlepl dpmtqsslfs ftelfsfmig tqtstgrrvp
      361 pvqkkellrv fkkavleval gkqmldkipv ckrdltylnr vlvitlhlac lltreipekg
      421 tdeyitlhka iyelvginak gkqgrdvlql iysekgavvg systskfpss nlanalikvg
      481 advtardndg ntalhlaavs hfrrpdlavt lleagahidt vnnegktfem llrdkrlyds
      541 vcpvkyttlt claarvvkrt yeledvpkhl rafvqmh
//
LOCUS       XP_034195446             575 aa            linear   INV 03-SEP-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein rumpelstiltskin isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034195446
VERSION     XP_034195446.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339555.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="heterogeneous nuclear ribonucleoprotein
                     rumpelstiltskin isoform X1"
                     /calculated_mol_wt=62014
     Region          50..376
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          56..131
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          218..291
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          508..573
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..575
                     /gene="rump"
                     /coded_by="XM_034339555.2:340..2067"
                     /db_xref="GeneID:117611576"
ORIGIN      
        1 mikqeenpaq nsderdrsre rdrnrrserq nrinstsrdr srerndrggr kpsdrriyvs
       61 nipydfrwqd lkdlfrtevg kvahvelftd endkprgcgi vefedsdsvk iavekmhryd
      121 ikgrklvvke vdfdverdky grlatarnnd rvrddrfrdp prpqgggrqn mnppaggggg
      181 gggggggggg dnkfgntygl stqfleslgi ngplvtrvfv anldykvdek kllevfklag
      241 kvlhvelgkd kdgksrgfgv veydhpvesv qaismlhnqq lydrrmtvrl dranepdmpp
      301 klpeglkgig mglgaggnrl mdvarnipnv qannppvvnp isapvlaaga fgaglnnvvp
      361 aqlasaltnt naalqasfag glganlasss llnssltnel asnlnnfggg vggvgglsnl
      421 qasltggqsn nsfasrglsk mdndvgfggn nafggsnfgg grdfdggfnr gdndrvpgag
      481 ggfsgnqsqg gggnrqntng srpmsdtili gnlppnttwq mlrdkfqdvg evkfaemrgt
      541 dmgmvrfase wdaeravsmm nrsridgrti dvrly
//
LOCUS       XP_034195447             574 aa            linear   INV 03-SEP-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein rumpelstiltskin isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034195447
VERSION     XP_034195447.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339556.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..574
                     /product="heterogeneous nuclear ribonucleoprotein
                     rumpelstiltskin isoform X2"
                     /calculated_mol_wt=61915
     Region          50..375
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          56..131
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          217..290
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          507..572
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..574
                     /gene="rump"
                     /coded_by="XM_034339556.2:337..2061"
                     /db_xref="GeneID:117611576"
ORIGIN      
        1 mikqeenpaq nsderdrsre rdrnrrserq nrinstsrdr srerndrggr kpsdrriyvs
       61 nipydfrwqd lkdlfrtevg kvahvelftd endkprgcgi vefedsdsvk iavekmhryd
      121 ikgrklvvke dfdverdkyg rlatarnndr vrddrfrdpp rpqgggrqnm nppagggggg
      181 gggggggggd nkfgntygls tqfleslgin gplvtrvfva nldykvdekk llevfklagk
      241 vlhvelgkdk dgksrgfgvv eydhpvesvq aismlhnqql ydrrmtvrld ranepdmppk
      301 lpeglkgigm glgaggnrlm dvarnipnvq annppvvnpi sapvlaagaf gaglnnvvpa
      361 qlasaltntn aalqasfagg lganlasssl lnssltnela snlnnfgggv ggvgglsnlq
      421 asltggqsnn sfasrglskm dndvgfggnn afggsnfggg rdfdggfnrg dndrvpgagg
      481 gfsgnqsqgg ggnrqntngs rpmsdtilig nlppnttwqm lrdkfqdvge vkfaemrgtd
      541 mgmvrfasew daeravsmmn rsridgrtid vrly
//
LOCUS       XP_034195448             545 aa            linear   INV 03-SEP-2025
DEFINITION  heterogeneous nuclear ribonucleoprotein rumpelstiltskin isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034195448
VERSION     XP_034195448.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339557.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..545
                     /product="heterogeneous nuclear ribonucleoprotein
                     rumpelstiltskin isoform X3"
                     /calculated_mol_wt=58963
     Region          50..376
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          56..131
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          218..291
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          478..543
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..545
                     /gene="rump"
                     /coded_by="XM_034339557.2:341..1978"
                     /db_xref="GeneID:117611576"
ORIGIN      
        1 mikqeenpaq nsderdrsre rdrnrrserq nrinstsrdr srerndrggr kpsdrriyvs
       61 nipydfrwqd lkdlfrtevg kvahvelftd endkprgcgi vefedsdsvk iavekmhryd
      121 ikgrklvvke vdfdverdky grlatarnnd rvrddrfrdp prpqgggrqn mnppaggggg
      181 gggggggggg dnkfgntygl stqfleslgi ngplvtrvfv anldykvdek kllevfklag
      241 kvlhvelgkd kdgksrgfgv veydhpvesv qaismlhnqq lydrrmtvrl dranepdmpp
      301 klpeglkgig mglgaggnrl mdvarnipnv qannppvvnp isapvlaaga fgaglnnvvp
      361 aqlasaltnt naalqasfag glganlasss llnssltnel asnlnnfggg vggvggfggn
      421 nafggsnfgg grdfdggfnr gdndrvpgag ggfsgnqsqg gggnrqntng srpmsdtili
      481 gnlppnttwq mlrdkfqdvg evkfaemrgt dmgmvrfase wdaeravsmm nrsridgrti
      541 dvrly
//
LOCUS       XP_034195450             458 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611577 [Osmia lignaria lignaria].
ACCESSION   XP_034195450
VERSION     XP_034195450.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339559.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195450.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..458
                     /product="uncharacterized protein LOC117611577"
                     /calculated_mol_wt=52011
     CDS             1..458
                     /gene="LOC117611577"
                     /coded_by="XM_034339559.2:1..1377"
                     /db_xref="GeneID:117611577"
ORIGIN      
        1 mttttgdpst lkspaslsgg dlvsrllaat ppylynvplt phsfffseml rsfvqaktea
       61 sstsnvtnap rrrkrswrda rdrplelttk erqhhhhhhh hhpqqppqek yfhsqqqqqq
      121 qpearlesrs kqsdgydaes kvgnfeqkps fggdilktpd etksdfckqs knffderprh
      181 feqaqppenm ftgelqkvkp eeshssdrkn cnyndrstyd ertkstvgnq elplltdqkk
      241 ldfsrnflpg lpgrgcdmlp avkgfglpgn vtnpeflpsp lwyppypipq sypgidplhf
      301 fidlrvsghi wdrklserql pfkgkhcsaf svpqskeyns nrplnltrde astsrssedn
      361 prgtnyilrq ltrtyrdigq arkasrsets isgesedssk dvdnnerspk pedassastn
      421 teeerkdlra liglelvvdy vkepkgdpsn eqtsqvte
//
LOCUS       XP_034195451             358 aa            linear   INV 03-SEP-2025
DEFINITION  isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Osmia
            lignaria lignaria].
ACCESSION   XP_034195451
VERSION     XP_034195451.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339560.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..358
                     /product="isocitrate dehydrogenase [NAD] subunit alpha,
                     mitochondrial"
                     /calculated_mol_wt=38851
     Region          23..353
                     /region_name="Iso_dh"
                     /note="Isocitrate/isopropylmalate dehydrogenase; cl00445"
                     /db_xref="CDD:444908"
     CDS             1..358
                     /gene="Idh3a"
                     /coded_by="XM_034339560.2:133..1209"
                     /db_xref="GeneID:117611579"
ORIGIN      
        1 maahwmrnav pkigsvrfys skvhkctlip gdgigpeisa avqkifdaak vpiewesvdv
       61 tpvkgpdgkf gipqaaidsv nknkiglkgp lmtpigkghr slnlalrkef nlyanvrpcl
      121 slegyktlyd nvdvvtiren tegeysgieh eivegvvqsi kliteeassr vaefafqyaq
      181 dnnrkkvtav hkanimrmsd glflrccrda aqkfpsikfe eryldtvcln mvqdpsqydv
      241 lvmpnlygdi lsdmcaglvg glgltpsgni glngalfesv hgtapdiagq dkanptalll
      301 savmmlkymg lnqhakiien aaydtikegk yltgdlggta kcseytneic kkvsaisk
//
LOCUS       XP_034195454             142 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611581 [Osmia lignaria lignaria].
ACCESSION   XP_034195454
VERSION     XP_034195454.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339563.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195454.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..142
                     /product="uncharacterized protein LOC117611581"
                     /calculated_mol_wt=15925
     CDS             1..142
                     /gene="LOC117611581"
                     /coded_by="XM_034339563.2:117..545"
                     /db_xref="GeneID:117611581"
ORIGIN      
        1 mkrnqtflli vlaiivvavn grvyylptld enrysgltyp ipneqssrdl dlnfsagdad
       61 efdnkirpvk rvytvmgpek pfievnkeel pitryklvea pvkgldnviv vpessrksvk
      121 idgnnkgevi lelrvianhd tv
//
LOCUS       XP_076546688             843 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143305761 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546688
VERSION     XP_076546688.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690573.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio            :: 35% of CDS bases
            internal stop codons :: corrected 1 genomic stop codon
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..843
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143305761"
                     /calculated_mol_wt=97858
     Region          281..449
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(336..341,370..371,398,400..401,445..446)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(336..341,370,400)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            371
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          544..662
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(547..550,583..585,588,613,646,660)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     CDS             1..843
                     /gene="LOC143305761"
                     /coded_by="XM_076690573.1:1..2532"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: substituted 1 base at 1
                     genomic stop codon"
                     /db_xref="GeneID:143305761"
ORIGIN      
        1 mekeirklre kvenlreelr swrleekrsk ekekdrrvke rdddeeevag eetdgknken
       61 eerdykekgn revmrgkslk eeyqeesekk ieekgesrke tkgrvempne avvdyssrvs
      121 sillqiqncn eievpehtqq ynvsaeanav kafltrikpe ifsrmrncef dtlneaisaa
      181 ikgeveyken mmrmkltegy tqavqcsncf gyghstntcs rrsfqrqqks mdttkpgstk
      241 pnpgepaakh rsesnkptpr ngtttgfsts gkpsfgesdf ltpvpkkent qgnkswrlvi
      301 dfrklnekti sdayhlpniv dildqlgsak yfsifdlkss fhqipmhpnd khktafstpf
      361 ghfqskrmpf glknaagsfq rlmnnfldrl qgveafvyid diviyassie ehetkfnrlm
      421 arlreanlyl qpekcellkk evaylghiit edgvkpdpkk ieavkefpvp ktvknirqfl
      481 glcgyyrxii infskvakpl sdltkkehkf ewtqqqqqaf iilrdklcee pilqypdftq
      541 pfnittdasg iavgavlsqg eigkdqpvsy tsrvlnkget qysaterell avvyavthfk
      601 pyiygrkfyl itdhkpltwl hnltdlasrl mrwklklsey dyeikykpgk qninadalsr
      661 npivtlpiqa krkhsttdsk evkkkkqwhq yatrpratts ttesreikkk kqwhqyptrp
      721 rsttsttgsk kvtkrktewh qyptrprpst tsdsrehkkr kethqyptrp rpsttsdsre
      781 ykkkkethqh ptrprsttss gsrevkkkne ihqyptrrrd tsselnketk kkkqilpnlk
      841 evl
//
LOCUS       XP_076546689             138 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305762 [Osmia lignaria lignaria].
ACCESSION   XP_076546689
VERSION     XP_076546689.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690574.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..138
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..138
                     /product="uncharacterized protein LOC143305762"
                     /calculated_mol_wt=16134
     CDS             1..138
                     /gene="LOC143305762"
                     /coded_by="XM_076690574.1:7..423"
                     /db_xref="GeneID:143305762"
ORIGIN      
        1 mrereslril lfqshltdsf difvkrfdql rknyrvvrhr splspspmps srrrvvqmki
       61 sstrrrrsig vviqkifrmv dnnaaaeapr aevpaeikki rkmarremnk tlrkvrkltf
      121 krrppppaay pggkilse
//
LOCUS       XP_076546690            1356 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305763 [Osmia lignaria lignaria].
ACCESSION   XP_076546690
VERSION     XP_076546690.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690575.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 43% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1356
                     /product="uncharacterized protein LOC143305763"
                     /calculated_mol_wt=155229
     Region          223..310
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(235,237,239,263..264,304)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            235..237
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            235
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(263..265,269..272)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          433..>563
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(496..501,530)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            531
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          539..617
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(546,548,550,573..575,611)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            546..548
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            546
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(573..576,580..583)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          837..1015
                     /region_name="RT_LTR"
                     /note="Reverse transcriptases (RTs) from retrotransposons
                     and retroviruses which have long terminal repeats (LTRs)
                     in their DNA copies but not in their RNA template. RT
                     catalyzes DNA replication from an RNA template, and is
                     responsible for the replication of...; cd01647"
                     /db_xref="CDD:238825"
     Site            order(905..910,939..940,965,967..968,1012..1013)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238825"
     Site            order(905..910,939,967)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238825"
     Site            940
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238825"
     Region          1110..1229
                     /region_name="RNase_HI_RT_Ty3"
                     /note="Ty3/Gypsy family of RNase HI in long-term repeat
                     retroelements; cd09274"
                     /db_xref="CDD:260006"
     Site            order(1114..1117,1150..1152,1155,1180,1213,1227)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260006"
     CDS             1..1356
                     /gene="LOC143305763"
                     /coded_by="XM_076690575.1:1..4071"
                     /db_xref="GeneID:143305763"
ORIGIN      
        1 mlfsdnlran sfrasraqfr iskqqverlr vmyqlddcas stmimlrlrg kglkwfhskp
       61 slmelsatal leemrkmfev rldklaarre fekrtwqkte sfcdyfhdkv tlanrilidp
      121 eelvdlvidg vpdprlrdqa rmqrletasd lleafrklsl rdevssdreg lqktstgmgi
      181 llqmrssasr kelpekyphg gpgakaatgt vvdidinids dagnfckfai tamidsgssv
      241 slikygllps alrldspkln nyyginhsrl dilgvfstma nindmrleik ffvvldntms
      301 cfallgrdfi ssrsvritmg dafeiatssn kdheletknf agqilqidyv etpitvtenl
      361 nidpnldskt akelrqiyld iqnhpidnne ppcsveavia lkteqpvsfr prrlafadke
      421 klriilddll kqgiirlsns pyaspivmvr kkngeirlcv dfreinklti rdnfpvplid
      481 dyldqlrdkq yftkldlkda fhhvrvsens vkytsfitpl gqyeylkmpf gltnapqlet
      541 aeflidggaa vnlivrdvlg dqakritkev ieitgltksp iysigttilh ihgkpalfyv
      601 venlaieadg iigspfllqe eaeisyyhgt lvlkdkpirp lrfsnyrdla qqknpkstkh
      661 riearttkqi aihianpnik egylprlkts eniyigeaav ynrdgicyal atntgeddvd
      721 ieiepqyihp ydiydssddd pissymtent penratrirn llrtdhlnae erkhvfnivd
      781 efsdrfylpg dklgevpnfy hsihttddvp intrqyrypp vhqdeiqrqv kalllqgviq
      841 pssspynspl wivpkkpdak gnkrwrmvid fralnektls dkfplpnite ildklggaky
      901 ftifdiangf hqvqmdpkdc pktafttpdg hfefvrmpfg lknapptfqr lmnqvisgle
      961 nahvfiddmv vfssslrehe ikvkkllnrl refgltlqid kceflrkevt ylghilteng
     1021 vkpdprklqa vkefpipktq knvqqflglt gyyrrfikdy sikskplvql lgkgirfkwt
     1081 edqqksfenl rdelctqpil qypdfnrpfi vttdasdyai gavlsqgeig ndlpiayasr
     1141 tlnkaernys atekeclamv yavqyfrpyl ygtkftlvtd hrplvwlhsv kdptsrlmkw
     1201 rlrlleyeyq vihkagktnk nadalsrnpv psclpliprd pqdpdykhig qkpeidtdsi
     1261 gfrvrqlqrd rerrpkyqee ntssdeepar gkvqhspiad rprvlpnlsh eismpdehme
     1321 tinfhpqans tkitsteedr lgpqeqtvrs ngstvl
//
LOCUS       XP_034195319             213 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611480 [Osmia lignaria lignaria].
ACCESSION   XP_034195319
VERSION     XP_034195319.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339428.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195319.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..213
                     /product="uncharacterized protein LOC117611480"
                     /calculated_mol_wt=24834
     CDS             1..213
                     /gene="LOC117611480"
                     /coded_by="XM_034339428.2:1..642"
                     /db_xref="GeneID:117611480"
ORIGIN      
        1 miniiekare qhyikkdwts rqqlipgekn ikhtnlvdpq nvylprlhik lgimknfvka
       61 mdksgpgfey lktkfpsisd akikegifvg pqirnlmndk nftlklnnle kavwpvfkkt
      121 thnffgnhra rnyisivnkl lkackelgcn msltiyflhs hldffppnlg dvsdehgecf
      181 hqdifrmekr yqgkkscaml adycwtlike kkm
//
LOCUS       XP_034195547             529 aa            linear   INV 03-SEP-2025
DEFINITION  potassium/sodium hyperpolarization-activated cyclic
            nucleotide-gated channel 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195547
VERSION     XP_034195547.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339656.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..529
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 2 isoform X1"
                     /calculated_mol_wt=62303
     Region          63..>470
                     /region_name="PLN03192"
                     /note="Voltage-dependent potassium channel; Provisional"
                     /db_xref="CDD:215625"
     Region          402..508
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(467..468,478..480)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(500..502,506..508)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     CDS             1..529
                     /gene="LOC117611641"
                     /coded_by="XM_034339656.2:132..1721"
                     /db_xref="GeneID:117611641"
ORIGIN      
        1 mlhvehqcei iakeadvlfv pgrgrfrkmy dfirlsmias rnnpaahkyl rssasivyek
       61 rrhlrmflyi ihpfsmfrhf wdflmifmmt svlilvpyqa tfemirkska wiasknfllv
      121 fccadivvnf mtgyfdkeeh gvileqqkva hhyiksstfi fdflgsfptd lffvttwyqy
      181 tvirktlsli cifrvfslgl yidriafayd iplalyefck ilfwlmmalh wqsclhwlip
      241 iaatsmylpq rpkndswlha gnlweaprnq qylnslmrav ttflrggmlr tnprhvgdiy
      301 liitlqligi vspwflitrv mqffkganss rlryqgtvaq lkqymrhrql pyptqrriie
      361 yyefrfqhrf freteiihtl sshmrneism hscrklvenv tffnnlplsl lsrivsllrs
      421 eifltndviv ranqpgdcmy figtgtvaiy tnsgkevchl edgahfgeva lvmpnemrva
      481 sviavevcel yrlnradfar tihpypmlwe sikkiaierh ektiilnaq
//
LOCUS       XP_076546692             445 aa            linear   INV 03-SEP-2025
DEFINITION  potassium/sodium hyperpolarization-activated cyclic
            nucleotide-gated channel 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546692
VERSION     XP_076546692.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690577.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..445
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 2 isoform X2"
                     /calculated_mol_wt=52076
     Region          1..>386
                     /region_name="PLN03192"
                     /note="Voltage-dependent potassium channel; Provisional"
                     /db_xref="CDD:215625"
     Region          318..424
                     /region_name="CAP_ED"
                     /note="effector domain of the CAP family of transcription
                     factors; members include CAP (or cAMP receptor protein
                     (CRP)), which binds cAMP, FNR (fumarate and nitrate
                     reduction), which uses an iron-sulfur cluster to sense
                     oxygen) and CooA, a heme containing CO...; cd00038"
                     /db_xref="CDD:237999"
     Site            order(383..384,394..396)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:237999"
     Site            order(416..418,422..424)
                     /site_type="other"
                     /note="flexible hinge region"
                     /db_xref="CDD:237999"
     CDS             1..445
                     /gene="LOC117611641"
                     /coded_by="XM_076690577.1:168..1505"
                     /db_xref="GeneID:117611641"
ORIGIN      
        1 mifmmtsvli lvpyqatfem irkskawias knfllvfcca divvnfmtgy fdkeehgvil
       61 eqqkvahhyi ksstfifdfl gsfptdlffv ttwyqytvir ktlslicifr vfslglyidr
      121 iafaydipla lyefckilfw lmmalhwqsc lhwlipiaat smylpqrpkn dswlhagnlw
      181 eaprnqqyln slmravttfl rggmlrtnpr hvgdiyliit lqligivspw flitrvmqff
      241 kganssrlry qgtvaqlkqy mrhrqlpypt qrriieyyef rfqhrffret eiihtlsshm
      301 rneismhscr klvenvtffn nlplsllsri vsllrseifl tndvivranq pgdcmyfigt
      361 gtvaiytnsg kevchledga hfgevalvmp nemrvasvia vevcelyrln radfartihp
      421 ypmlwesikk iaierhekti ilnaq
//
LOCUS       XP_034194206             100 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610678 [Osmia lignaria lignaria].
ACCESSION   XP_034194206
VERSION     XP_034194206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338315.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..100
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..100
                     /product="uncharacterized protein LOC117610678"
                     /calculated_mol_wt=11413
     CDS             1..100
                     /gene="LOC117610678"
                     /coded_by="XM_034338315.2:353..655"
                     /db_xref="GeneID:117610678"
ORIGIN      
        1 mddstidqna imnalnmlqh iqqrqdaqqq eqqslqqnlq qallqqqqqp lqqeqqevkp
       61 qrkrgtaagt rhrpfrgkwp cggrgggrgg shnkyyvffk
//
LOCUS       XP_076546693             224 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC117610671 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546693
VERSION     XP_076546693.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690578.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 10% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..224
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC117610671"
                     /calculated_mol_wt=24812
     Region          41..206
                     /region_name="DNA_BRE_C"
                     /note="DNA breaking-rejoining enzymes, C-terminal
                     catalytic domain; cl00213"
                     /db_xref="CDD:469662"
     Site            order(55..56,144,146,166,203)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:271157"
     Site            order(55,166,169,192,203)
                     /site_type="active"
                     /db_xref="CDD:271157"
     CDS             1..224
                     /gene="LOC117610671"
                     /coded_by="XM_076690578.1:1..675"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:117610671"
ORIGIN      
        1 mdpelllfed slaprsinle lltkklvmll alatahsgtk lllaiavlsh caaqrvpslt
       61 kirisnikif dteirikisd riktsgpgrt qpmlqllffk eqsrlcvati lcqyikqsak
      121 irkqgedmll ltykkphgva tpqsvsrwlk svmaksgidt rnfgshstrh astsaasrrg
      181 vdieairraa gwteksntft rfynrpvikn tsvarsviga eiql
//
LOCUS       XP_034194194             202 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610667 [Osmia lignaria lignaria].
ACCESSION   XP_034194194
VERSION     XP_034194194.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338303.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="uncharacterized protein LOC117610667"
                     /calculated_mol_wt=23185
     Region          <81..156
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..202
                     /gene="LOC117610667"
                     /coded_by="XM_034338303.1:1..609"
                     /db_xref="GeneID:117610667"
ORIGIN      
        1 mncinfekwi tekllpnias nsetvmgnal yhsiqlnkpp nryakkadii nwlkdnnisy
       61 aetmrrnely nlvlahkkte ktfrvdeilk lnghtvfrlp pymcelnpie lawaqvkrti
      121 renntltlta selailtyka ieevtetnwe nfskhvekte ekscekdgll keamneltld
      181 ilstdsdhss dtdsaftmsd nv
//
LOCUS       XP_034195684            3223 aa            linear   INV 03-SEP-2025
DEFINITION  wing blister isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195684
VERSION     XP_034195684.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339793.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195684.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3223
                     /product="wing blister isoform X1"
                     /calculated_mol_wt=359232
     Region          59..285
                     /region_name="Laminin_N"
                     /note="Laminin N-terminal (Domain VI); pfam00055"
                     /db_xref="CDD:459653"
     Region          286..334
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(287,289,307,309,318)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          414..470
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(414,416,425,441,443,452)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          468..514
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(468,470,481,485,487,496)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          577..727
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          766..814
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(766,768,775,782,785,794)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          815..873
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(816,818,831,845,848,857)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          877..919
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(878,880,885,892,895,904)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          922..970
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(923,925,935,942,944,953)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          970..1017
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(970,972,981,988,990,999)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1017..1063
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1017,1019,1029,1036,1038,1047)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1065..1112
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1066,1068,1078,1085,1087,1096)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1114..1158
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(1114,1124,1131,1133,1142)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1161..1207
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1161,1163,1172,1178,1180,1189)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1205..1257
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1205,1207,1220,1228,1230,1239)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1343..1478
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1522..1570
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1522,1524,1531,1538,1541,1550)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          <1591..1620
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Region          1622..>1656
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1623,1625,1635,1642,1644,1653)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1722..2231
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          2267..2415
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          2446..2611
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2637..2803
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2890..3018
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          3042..3199
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     CDS             1..3223
                     /gene="wb"
                     /coded_by="XM_034339793.2:871..10542"
                     /db_xref="GeneID:117611699"
ORIGIN      
        1 mmavrvhhrs slmlpwlifl slvlwnlnda nagrgfrhst rhsqsglygt srnlknsglf
       61 pstfnvaaka difvnatcge egpetfckps essrcavcda rspdpgkrhn isnildsnpg
      121 rwwqsptlar gdhyeyvtiv ldlkqvyqie yvivkaansp rpaawilers idgenfepwq
      181 yyapsdeecw trysvppvsg kpvyigddev vctsvhsrqt pmengeihth lvngrpgaln
      241 hsttlqeftq aryvrlrlqg lrrngetiad krrafysike iniggrclcs ghaarcrysv
      301 hhghqecece rhtcgercek ccpmynqipw kpgtaakgfh cekcncngha tscrydqdva
      361 drrmsmdirg kfrgggvcin ctehtaginc encqvgyyrp ngvapdapep cipcdcnvyg
      421 stgyctpdds ythmgkvaga cecksgysgy kcdqcaagyr qfpdcmpcpc dsrgilpshd
      481 cegdclckan vagefcdhck pghfaltken ldgclscycf gisnrcstak ltyssissle
      541 dwlitdmnas raiipildtd sgwltvaafd veydspfwla priycgnrls sygsnltysv
      601 swvvmrgdts gkpttgpnvi lvgnngmria hgeeqyngqe aeitvalren gwyhvrseiq
      661 diptrqrrte frgdpvtriq miqvladlky lmiraqyhte qiegsfqsat lsigelvssd
      721 esnsfvevce cpegyiglsc encawgyvkv irnesdhqdq hvcvkcdcng hastcdlvlg
      781 ectscehntv gpkcdrcapg fygialkgtp ndckrcacpl lnesnnfspn cqlddptdad
      841 sgyvctqcpn gytgdhcesc digffgnpli pggtcepclc gggpcdqetg rclecrgnte
      901 gwkcdkckla hygnpleqnc lpcecdplgs ssvecdqrsg qcpcmplfeg rdcssciegy
      961 gnvtagcrec dcdvgaldev cdpvtgecrc adgvlgfrcd hcdvdhygls edgckgcrcn
     1021 tigsitsacd ivtgqcqckp hvtsrqcdhc vvgywglttg agcapcgcdp igshnsschd
     1081 vtgqcyckpg vggarcdtcl pgyygfsskg cqvcdpclrs ghicdpdtgr cvcptltfge
     1141 hcdrcrpgsw dlvhgvgcrp cdctlgstrp hcdhqgqcpc rigydgrrce kcakgyygyp
     1201 rcrpcgcnsa gtlqcgdeic dcddqgqcpc kelvygrqcn qcregtfgla vdspkgctec
     1261 fcfgrttscq qaglswgqrr ltrprtlyvn dtindiiitk frsqiylspi ngglnvtngl
     1321 siipdiegdv tipsnlyyny plywmlpeqf lgdkvvsygg flrfttsteg giplrstyqy
     1381 pvvqlqgnhr ivleyylplp vdnnhyevrf heslwqlqnr pdykvtrevl mvalqnlqyi
     1441 lvkasdnaef tkttllqasl daavltptht ppfatgieic kcppeynsts cqdpsigyyr
     1501 wyknttvtst ividlvgear rcqcngrsei cdketghcln crgntvgprc dvcadsfygh
     1561 pdygckpcpc pqtdkrfsnt cmilanteai cvckpgytgr kcercspgyy ghphlpdgkc
     1621 mpcncnsags lsdecdmetg qcrcragstg rdcsectayr hvyinnvcts cndnctglll
     1681 dtlailsqel aegtahiadg yipppwedla yidsnvtayf eeielqnklk qrmknipwhe
     1741 yrklknhlea lfrtaneqvk qgdtfksksn dlknnifsak iemdnlkkyi ddtiqlltqy
     1801 nsdnkrieik kalkmaklil nnlksvhltk ktmeienfle dvtdytdwln slydatepla
     1861 iaqddaeeya vklndmrnii edtmetlysy dtlynninqt llearnscse idmlrsavne
     1921 sinegeklid etrgfiidlq iniqdlpelk nkltywfdkl nmkeellyrl nyeykdkyvv
     1981 pavehaknls nyvdqyvslf setrniaasp lkasqaykni vdsirtanit akaakdivdd
     2041 iynktfpngp kskslldesk eislasskqw dsaekhgkiv qnasdqldfq kqavnalknt
     2101 lnntgvkdnq vnvklqslqn dserlreniq silddneevl etvkdnqrli ddynegianr
     2161 lkpklyelkr egdskislas ekltealsni kkadaklisl snaavkrknv fdkwndtlas
     2221 klqnlkdkia earntadgir vslrstegke cirsyrpstl qpsstntivm tfalpkprke
     2281 gplfylpssi nddfialeiv dkrvrfvwnv gggtgilvhp eviesgdlrn dtswyrieae
     2341 rmrhlgklyv rkqasaiggy ppvtnstdfe ygrfdvtaad rvwiggiprs qrrrtellan
     2401 nglpgcvhqv ildgkpiglw nfittapdma cracvegved vrddiaysfn gegyavrnrv
     2461 ssgpynkytf gvsinfrtyd enallflain pknyqhimif lregkvilhi gygsnvsmem
     2521 ssyskyntgn wtkvdafrqy hlrkniekcs lsvgdadkki gvptpqpkkd dipdfiqaky
     2581 yiggvppsfr aeklmlppqa sflgcmsnii vqegydpmae qyygveptcg nkplkivgfy
     2641 gdgylehaay tlrkinstvs fsfrtmqtea vlllstfegq eervtnirhg neerdsyysv
     2701 clingqvqvr lnagrgelvl qsndtfndgr yhsvtiikrr kdvelridda yqtsgrspts
     2761 aaikapegng glffgglpvl inntkmittt tplygaikda vfnediirfd evvsfdhali
     2821 grpgpsmgkd pptytpsasl srglstqpeg cqkvpyysle pgalkfgdkp nshtqlylnf
     2881 kkfwekkyai efdfrtyypn gllfitpgvr pkhylmvvir dgqllllvks kqkkeilfkt
     2941 pfndgnwhhv vishderklt llvdaqtprt ikvprkigla smmyigglpe sgtpipeqvv
     3001 vkletlkgci rglrvngnvy dmvgstsray hvgqcfpnve sgayfqdeay aiykrnfelg
     3061 avlelqlefr tselsgvlls itapgnspsl slelnngkvi msgdlgdnnp lyveqrftsp
     3121 yticdnrwhr iqavyndeel alkvdemdqk yglpanvnyh imdstvsgpl yigglpasap
     3181 kgtlitrdhf ngcirnvmig gerrdwtdmd elhnihlssc pvq
//
LOCUS       XP_034195685            3144 aa            linear   INV 03-SEP-2025
DEFINITION  wing blister isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195685
VERSION     XP_034195685.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339794.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195685.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3144
                     /product="wing blister isoform X2"
                     /calculated_mol_wt=350287
     Region          59..285
                     /region_name="Laminin_N"
                     /note="Laminin N-terminal (Domain VI); pfam00055"
                     /db_xref="CDD:459653"
     Region          335..391
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(335,337,346,362,364,373)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          389..435
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(389,391,402,406,408,417)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          498..648
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          687..735
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(687,689,696,703,706,715)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          736..794
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(737,739,752,766,769,778)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          798..840
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(799,801,806,813,816,825)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          843..891
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(844,846,856,863,865,874)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          891..938
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(891,893,902,909,911,920)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          938..984
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(938,940,950,957,959,968)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          986..1033
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(987,989,999,1006,1008,1017)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1035..1079
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(1035,1045,1052,1054,1063)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1082..1128
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1082,1084,1093,1099,1101,1110)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1126..1178
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1126,1128,1141,1149,1151,1160)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1264..1399
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1443..1491
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1443,1445,1452,1459,1462,1471)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          <1512..1541
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Region          1543..>1577
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1544,1546,1556,1563,1565,1574)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1643..2152
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          2188..2336
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          2367..2532
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2558..2724
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2811..2939
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          2963..3120
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     CDS             1..3144
                     /gene="wb"
                     /coded_by="XM_034339794.2:871..10305"
                     /db_xref="GeneID:117611699"
ORIGIN      
        1 mmavrvhhrs slmlpwlifl slvlwnlnda nagrgfrhst rhsqsglygt srnlknsglf
       61 pstfnvaaka difvnatcge egpetfckps essrcavcda rspdpgkrhn isnildsnpg
      121 rwwqsptlar gdhyeyvtiv ldlkqvyqie yvivkaansp rpaawilers idgenfepwq
      181 yyapsdeecw trysvppvsg kpvyigddev vctsvhsrqt pmengeihth lvngrpgaln
      241 hsttlqeftq aryvrlrlqg lrrngetiad krrafysike iniggrclcs ghaarcrysv
      301 hhgehtagin cencqvgyyr pngvapdape pcipcdcnvy gstgyctpdd sythmgkvag
      361 acecksgysg ykcdqcaagy rqfpdcmpcp cdsrgilpsh dcegdclcka nvagefcdhc
      421 kpghfaltke nldgclscyc fgisnrcsta kltyssissl edwlitdmna sraiipildt
      481 dsgwltvaaf dveydspfwl apriycgnrl ssygsnltys vswvvmrgdt sgkpttgpnv
      541 ilvgnngmri ahgeeqyngq eaeitvalre ngwyhvrsei qdiptrqrrt efrgdpvtri
      601 qmiqvladlk ylmiraqyht eqiegsfqsa tlsigelvss desnsfvevc ecpegyigls
      661 cencawgyvk virnesdhqd qhvcvkcdcn ghastcdlvl gectscehnt vgpkcdrcap
      721 gfygialkgt pndckrcacp llnesnnfsp ncqlddptda dsgyvctqcp ngytgdhces
      781 cdigffgnpl ipggtcepcl cgggpcdqet grclecrgnt egwkcdkckl ahygnpleqn
      841 clpcecdplg sssvecdqrs gqcpcmplfe grdcsscieg ygnvtagcre cdcdvgalde
      901 vcdpvtgecr cadgvlgfrc dhcdvdhygl sedgckgcrc ntigsitsac divtgqcqck
      961 phvtsrqcdh cvvgywgltt gagcapcgcd pigshnssch dvtgqcyckp gvggarcdtc
     1021 lpgyygfssk gcqvcdpclr sghicdpdtg rcvcptltfg ehcdrcrpgs wdlvhgvgcr
     1081 pcdctlgstr phcdhqgqcp crigydgrrc ekcakgyygy prcrpcgcns agtlqcgdei
     1141 cdcddqgqcp ckelvygrqc nqcregtfgl avdspkgcte cfcfgrttsc qqaglswgqr
     1201 rltrprtlyv ndtindiiit kfrsqiylsp ingglnvtng lsiipdiegd vtipsnlyyn
     1261 yplywmlpeq flgdkvvsyg gflrfttste ggiplrstyq ypvvqlqgnh rivleyylpl
     1321 pvdnnhyevr fheslwqlqn rpdykvtrev lmvalqnlqy ilvkasdnae ftkttllqas
     1381 ldaavltpth tppfatgiei ckcppeynst scqdpsigyy rwyknttvts tividlvgea
     1441 rrcqcngrse icdketghcl ncrgntvgpr cdvcadsfyg hpdygckpcp cpqtdkrfsn
     1501 tcmilantea icvckpgytg rkcercspgy yghphlpdgk cmpcncnsag slsdecdmet
     1561 gqcrcragst grdcsectay rhvyinnvct scndnctgll ldtlailsqe laegtahiad
     1621 gyipppwedl ayidsnvtay feeielqnkl kqrmknipwh eyrklknhle alfrtaneqv
     1681 kqgdtfksks ndlknnifsa kiemdnlkky iddtiqlltq ynsdnkriei kkalkmakli
     1741 lnnlksvhlt kktmeienfl edvtdytdwl nslydatepl aiaqddaeey avklndmrni
     1801 iedtmetlys ydtlynninq tllearnscs eidmlrsavn esinegekli detrgfiidl
     1861 qiniqdlpel knkltywfdk lnmkeellyr lnyeykdkyv vpavehaknl snyvdqyvsl
     1921 fsetrniaas plkasqaykn ivdsirtani takaakdivd diynktfpng pkskslldes
     1981 keislasskq wdsaekhgki vqnasdqldf qkqavnalkn tlnntgvkdn qvnvklqslq
     2041 ndserlreni qsilddneev letvkdnqrl iddynegian rlkpklyelk regdskisla
     2101 sekltealsn ikkadaklis lsnaavkrkn vfdkwndtla sklqnlkdki aearntadgi
     2161 rvslrstegk ecirsyrpst lqpsstntiv mtfalpkprk egplfylpss inddfialei
     2221 vdkrvrfvwn vgggtgilvh peviesgdlr ndtswyriea ermrhlgkly vrkqasaigg
     2281 yppvtnstdf eygrfdvtaa drvwiggipr sqrrrtella nnglpgcvhq vildgkpigl
     2341 wnfittapdm acracvegve dvrddiaysf ngegyavrnr vssgpynkyt fgvsinfrty
     2401 denallflai npknyqhimi flregkvilh igygsnvsme mssyskyntg nwtkvdafrq
     2461 yhlrkniekc slsvgdadkk igvptpqpkk ddipdfiqak yyiggvppsf raeklmlppq
     2521 asflgcmsni ivqegydpma eqyygveptc gnkplkivgf ygdgylehaa ytlrkinstv
     2581 sfsfrtmqte avlllstfeg qeervtnirh gneerdsyys vclingqvqv rlnagrgelv
     2641 lqsndtfndg ryhsvtiikr rkdvelridd ayqtsgrspt saaikapegn gglffgglpv
     2701 linntkmitt ttplygaikd avfnediirf devvsfdhal igrpgpsmgk dpptytpsas
     2761 lsrglstqpe gcqkvpyysl epgalkfgdk pnshtqlyln fkkfwekkya iefdfrtyyp
     2821 ngllfitpgv rpkhylmvvi rdgqllllvk skqkkeilfk tpfndgnwhh vvishderkl
     2881 tllvdaqtpr tikvprkigl asmmyigglp esgtpipeqv vvkletlkgc irglrvngnv
     2941 ydmvgstsra yhvgqcfpnv esgayfqdea yaiykrnfel gavlelqlef rtselsgvll
     3001 sitapgnsps lslelnngkv imsgdlgdnn plyveqrfts pyticdnrwh riqavyndee
     3061 lalkvdemdq kyglpanvny himdstvsgp lyigglpasa pkgtlitrdh fngcirnvmi
     3121 ggerrdwtdm delhnihlss cpvq
//
LOCUS       XP_034195686            2758 aa            linear   INV 03-SEP-2025
DEFINITION  wing blister isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195686
VERSION     XP_034195686.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339795.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195686.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2758
                     /product="wing blister isoform X3"
                     /calculated_mol_wt=307284
     Region          3..49
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(3,5,16,20,22,31)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          112..262
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          301..349
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(301,303,310,317,320,329)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          350..408
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(351,353,366,380,383,392)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          412..454
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(413,415,420,427,430,439)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          457..505
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(458,460,470,477,479,488)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          505..552
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(505,507,516,523,525,534)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          552..598
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(552,554,564,571,573,582)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          600..647
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(601,603,613,620,622,631)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          649..693
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(649,659,666,668,677)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          696..742
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(696,698,707,713,715,724)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          740..792
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(740,742,755,763,765,774)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          878..1013
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1057..1105
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1057,1059,1066,1073,1076,1085)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          <1126..1155
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Region          1157..>1191
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1158,1160,1170,1177,1179,1188)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1257..1766
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          1802..1950
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          1981..2146
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2172..2338
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2425..2553
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          2577..2734
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     CDS             1..2758
                     /gene="wb"
                     /coded_by="XM_034339795.2:224..8500"
                     /db_xref="GeneID:117611699"
ORIGIN      
        1 mpcpcdsrgi lpshdcegdc lckanvagef cdhckpghfa ltkenldgcl scycfgisnr
       61 cstakltyss issledwlit dmnasraiip ildtdsgwlt vaafdveyds pfwlapriyc
      121 gnrlssygsn ltysvswvvm rgdtsgkptt gpnvilvgnn gmriahgeeq yngqeaeitv
      181 alrengwyhv rseiqdiptr qrrtefrgdp vtriqmiqvl adlkylmira qyhteqiegs
      241 fqsatlsige lvssdesnsf vevcecpegy iglscencaw gyvkvirnes dhqdqhvcvk
      301 cdcnghastc dlvlgectsc ehntvgpkcd rcapgfygia lkgtpndckr cacpllnesn
      361 nfspncqldd ptdadsgyvc tqcpngytgd hcescdigff gnplipggtc epclcgggpc
      421 dqetgrclec rgntegwkcd kcklahygnp leqnclpcec dplgsssvec dqrsgqcpcm
      481 plfegrdcss ciegygnvta gcrecdcdvg aldevcdpvt gecrcadgvl gfrcdhcdvd
      541 hyglsedgck gcrcntigsi tsacdivtgq cqckphvtsr qcdhcvvgyw glttgagcap
      601 cgcdpigshn sschdvtgqc yckpgvggar cdtclpgyyg fsskgcqvcd pclrsghicd
      661 pdtgrcvcpt ltfgehcdrc rpgswdlvhg vgcrpcdctl gstrphcdhq gqcpcrigyd
      721 grrcekcakg yygyprcrpc gcnsagtlqc gdeicdcddq gqcpckelvy grqcnqcreg
      781 tfglavdspk gctecfcfgr ttscqqagls wgqrrltrpr tlyvndtind iiitkfrsqi
      841 ylspinggln vtnglsiipd iegdvtipsn lyynyplywm lpeqflgdkv vsyggflrft
      901 tsteggiplr styqypvvql qgnhrivley ylplpvdnnh yevrfheslw qlqnrpdykv
      961 trevlmvalq nlqyilvkas dnaeftkttl lqasldaavl tpthtppfat gieickcppe
     1021 ynstscqdps igyyrwyknt tvtstividl vgearrcqcn grseicdket ghclncrgnt
     1081 vgprcdvcad sfyghpdygc kpcpcpqtdk rfsntcmila nteaicvckp gytgrkcerc
     1141 spgyyghphl pdgkcmpcnc nsagslsdec dmetgqcrcr agstgrdcse ctayrhvyin
     1201 nvctscndnc tgllldtlai lsqelaegta hiadgyippp wedlayidsn vtayfeeiel
     1261 qnklkqrmkn ipwheyrklk nhlealfrta neqvkqgdtf ksksndlknn ifsakiemdn
     1321 lkkyiddtiq lltqynsdnk rieikkalkm aklilnnlks vhltkktmei enfledvtdy
     1381 tdwlnslyda teplaiaqdd aeeyavklnd mrniiedtme tlysydtlyn ninqtllear
     1441 nscseidmlr savnesineg eklidetrgf iidlqiniqd lpelknklty wfdklnmkee
     1501 llyrlnyeyk dkyvvpaveh aknlsnyvdq yvslfsetrn iaasplkasq ayknivdsir
     1561 tanitakaak divddiynkt fpngpksksl ldeskeisla sskqwdsaek hgkivqnasd
     1621 qldfqkqavn alkntlnntg vkdnqvnvkl qslqndserl reniqsildd neevletvkd
     1681 nqrliddyne gianrlkpkl yelkregdsk islaseklte alsnikkada klislsnaav
     1741 krknvfdkwn dtlasklqnl kdkiaearnt adgirvslrs tegkecirsy rpstlqpsst
     1801 ntivmtfalp kprkegplfy lpssinddfi aleivdkrvr fvwnvgggtg ilvhpevies
     1861 gdlrndtswy rieaermrhl gklyvrkqas aiggyppvtn stdfeygrfd vtaadrvwig
     1921 giprsqrrrt ellannglpg cvhqvildgk piglwnfitt apdmacracv egvedvrddi
     1981 aysfngegya vrnrvssgpy nkytfgvsin frtydenall flainpknyq himiflregk
     2041 vilhigygsn vsmemssysk yntgnwtkvd afrqyhlrkn iekcslsvgd adkkigvptp
     2101 qpkkddipdf iqakyyiggv ppsfraeklm lppqasflgc msniivqegy dpmaeqyygv
     2161 eptcgnkplk ivgfygdgyl ehaaytlrki nstvsfsfrt mqteavllls tfegqeervt
     2221 nirhgneerd syysvcling qvqvrlnagr gelvlqsndt fndgryhsvt iikrrkdvel
     2281 riddayqtsg rsptsaaika pegngglffg glpvlinntk mittttplyg aikdavfned
     2341 iirfdevvsf dhaligrpgp smgkdpptyt psaslsrgls tqpegcqkvp yyslepgalk
     2401 fgdkpnshtq lylnfkkfwe kkyaiefdfr tyypngllfi tpgvrpkhyl mvvirdgqll
     2461 llvkskqkke ilfktpfndg nwhhvvishd erkltllvda qtprtikvpr kiglasmmyi
     2521 gglpesgtpi peqvvvklet lkgcirglrv ngnvydmvgs tsrayhvgqc fpnvesgayf
     2581 qdeayaiykr nfelgavlel qlefrtsels gvllsitapg nspslsleln ngkvimsgdl
     2641 gdnnplyveq rftspyticd nrwhriqavy ndeelalkvd emdqkyglpa nvnyhimdst
     2701 vsgplyiggl pasapkgtli trdhfngcir nvmiggerrd wtdmdelhni hlsscpvq
//
LOCUS       XP_034195687            2726 aa            linear   INV 03-SEP-2025
DEFINITION  wing blister isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195687
VERSION     XP_034195687.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339796.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195687.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2726
                     /product="wing blister isoform X4"
                     /calculated_mol_wt=305598
     Region          289..379
                     /region_name="Cby_like"
                     /note="Chibby, a nuclear inhibitor of Wnt/beta-catenin
                     mediated transcription, and similar proteins; cl12007"
                     /db_xref="CDD:472255"
     Region          520..566
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(520,522,532,539,541,550)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          568..615
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(569,571,581,588,590,599)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          614..661
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(614,617,627,634,636,645)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          664..710
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(664,666,675,681,683,692)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          708..760
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(708,710,723,731,733,742)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          846..981
                     /region_name="Laminin_B"
                     /note="Laminin B (Domain IV); pfam00052"
                     /db_xref="CDD:459652"
     Region          1025..1073
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Site            order(1025,1027,1034,1041,1044,1053)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          <1094..1123
                     /region_name="Laminin_EGF"
                     /note="Laminin EGF domain; pfam00053"
                     /db_xref="CDD:395007"
     Region          1125..>1159
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domain;
                     laminins are the major noncollagenous components of
                     basement membranes that mediate cell adhesion, growth
                     migration, and differentiation; the laminin-type epidermal
                     growth factor-like module occurs in...; cd00055"
                     /db_xref="CDD:238012"
     Site            order(1126,1128,1138,1145,1147,1156)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          1223..1734
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     Region          1770..1918
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          1949..2114
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2140..2306
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          2393..2521
                     /region_name="LamG"
                     /note="Laminin G domain; smart00282"
                     /db_xref="CDD:214598"
     Region          2545..2702
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     CDS             1..2726
                     /gene="wb"
                     /coded_by="XM_034339796.2:1388..9568"
                     /db_xref="GeneID:117611699"
ORIGIN      
        1 mypgrsiwtr gvlalgvgal fllgwrisst gdassasase risslkidsn rnyfsvvlas
       61 srsprsrddd ddeeerkked plwqdrpveg dysagdaeld vehstsysae rslpfakdyr
      121 estslaeltd vstslknpen ddesgetrte ddnqfskdlg afngsagger shvnenssrq
      181 vngdsmnevv edfeptyyld fpeqtkiiat ssvkhfyste tdedvateiv sdfhnseesa
      241 ttkrergavd eesisgyppa fsrrrrltem qkdrapffrd qssantlesr kgrsserkih
      301 eggrsanrsp pavdlsldfr airgernnqm legdeltdpt gdgdyssrse rdssasfsdn
      361 vklpentple nlqidailef qnedytgesa vkkfqneens knigkaskss eiektylddr
      421 kvssidgrlg danlqrgknk vrrkrytnyy spqsvtpmay vhiqpvypva tpppanrkcv
      481 rcmvvykpcp smprqpprva lptykyqepa skwhglkygc rcntigsits acdivtgqcq
      541 ckphvtsrqc dhcvvgywgl ttgagcapcg cdpigshnss chdvtgqcyc kpgvggarcd
      601 tclpgyygfs skgcqvcdpc lrsghicdpd tgrcvcptlt fgehcdrcrp gswdlvhgvg
      661 crpcdctlgs trphcdhqgq cpcrigydgr rcekcakgyy gyprcrpcgc nsagtlqcgd
      721 eicdcddqgq cpckelvygr qcnqcregtf glavdspkgc tecfcfgrtt scqqaglswg
      781 qrrltrprtl yvndtindii itkfrsqiyl spingglnvt nglsiipdie gdvtipsnly
      841 ynyplywmlp eqflgdkvvs yggflrftts teggiplrst yqypvvqlqg nhrivleyyl
      901 plpvdnnhye vrfheslwql qnrpdykvtr evlmvalqnl qyilvkasdn aeftkttllq
      961 asldaavltp thtppfatgi eickcppeyn stscqdpsig yyrwyknttv tstividlvg
     1021 earrcqcngr seicdketgh clncrgntvg prcdvcadsf yghpdygckp cpcpqtdkrf
     1081 sntcmilant eaicvckpgy tgrkcercsp gyyghphlpd gkcmpcncns agslsdecdm
     1141 etgqcrcrag stgrdcsect ayrhvyinnv ctscndnctg llldtlails qelaegtahi
     1201 adgyipppwe dlayidsnvt ayfeeielqn klkqrmknip wheyrklknh lealfrtane
     1261 qvkqgdtfks ksndlknnif sakiemdnlk kyiddtiqll tqynsdnkri eikkalkmak
     1321 lilnnlksvh ltkktmeien fledvtdytd wlnslydate plaiaqddae eyavklndmr
     1381 niiedtmetl ysydtlynni nqtllearns cseidmlrsa vnesinegek lidetrgfii
     1441 dlqiniqdlp elknkltywf dklnmkeell yrlnyeykdk yvvpavehak nlsnyvdqyv
     1501 slfsetrnia asplkasqay knivdsirta nitakaakdi vddiynktfp ngpkskslld
     1561 eskeislass kqwdsaekhg kivqnasdql dfqkqavnal kntlnntgvk dnqvnvklqs
     1621 lqndserlre niqsilddne evletvkdnq rliddynegi anrlkpklye lkregdskis
     1681 laseklteal snikkadakl islsnaavkr knvfdkwndt lasklqnlkd kiaearntad
     1741 girvslrste gkecirsyrp stlqpsstnt ivmtfalpkp rkegplfylp ssinddfial
     1801 eivdkrvrfv wnvgggtgil vhpeviesgd lrndtswyri eaermrhlgk lyvrkqasai
     1861 ggyppvtnst dfeygrfdvt aadrvwiggi prsqrrrtel lannglpgcv hqvildgkpi
     1921 glwnfittap dmacracveg vedvrddiay sfngegyavr nrvssgpynk ytfgvsinfr
     1981 tydenallfl ainpknyqhi miflregkvi lhigygsnvs memssyskyn tgnwtkvdaf
     2041 rqyhlrknie kcslsvgdad kkigvptpqp kkddipdfiq akyyiggvpp sfraeklmlp
     2101 pqasflgcms niivqegydp maeqyygvep tcgnkplkiv gfygdgyleh aaytlrkins
     2161 tvsfsfrtmq teavlllstf egqeervtni rhgneerdsy ysvclingqv qvrlnagrge
     2221 lvlqsndtfn dgryhsvtii krrkdvelri ddayqtsgrs ptsaaikape gngglffggl
     2281 pvlinntkmi ttttplygai kdavfnedii rfdevvsfdh aligrpgpsm gkdpptytps
     2341 aslsrglstq pegcqkvpyy slepgalkfg dkpnshtqly lnfkkfwekk yaiefdfrty
     2401 ypngllfitp gvrpkhylmv virdgqllll vkskqkkeil fktpfndgnw hhvvishder
     2461 kltllvdaqt prtikvprki glasmmyigg lpesgtpipe qvvvkletlk gcirglrvng
     2521 nvydmvgsts rayhvgqcfp nvesgayfqd eayaiykrnf elgavlelql efrtselsgv
     2581 llsitapgns pslslelnng kvimsgdlgd nnplyveqrf tspyticdnr whriqavynd
     2641 eelalkvdem dqkyglpanv nyhimdstvs gplyigglpa sapkgtlitr dhfngcirnv
     2701 miggerrdwt dmdelhnihl sscpvq
//
LOCUS       XP_076546694            1763 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546694
VERSION     XP_076546694.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690579.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1763
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X1"
                     /calculated_mol_wt=197849
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          <1242..>1393
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1623..1741
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1763
                     /gene="Patronin"
                     /coded_by="XM_076690579.1:1162..6453"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtkgkvksps sstsggdspa eigpptpvts gssgdtptsv settlvtqqp
      781 sqekpqrpfs lkeisedvrd vehkwlehdg sapfietrrt pdienmdleq yhqsisqmnn
      841 slseiqadiq rlanqqnqiq qqhlmtqhqq qmqqqlqqlq slsqqhlqsf giapinplts
      901 klqdsqqqsq fylhdqpqlq rrmwgqpppt qslanemaav gyqqpmdpry gtqptstyim
      961 dqlikqsldk nkllgiynll yvtksiayqq dmrlyqdtrn wgthppqqkg fvlhdtpqep
     1021 rylnggdhsl cnnqmshpgs typssasifn qtppssaspq hrnavhrisq lmsespepkr
     1081 ptvhhipikc esptekrqvt alhapvpapp vddmkpqnis fignddeltq girglhitsg
     1141 srtyripspt rpsisrnsfq phpslreatp spsgtpevtp ldptdagekg fyicfdndap
     1201 kkpkptlrvk rtspkkersv ssyvdnedfm vrpespsvsa vdrqqkqlet hrdfdrekqr
     1261 qaderefqrq emrdrelqre verekqrerr etsaegrqsg vgliignqla npdpnsldem
     1321 erkkerimll slqrrqqqee mkerkeaeaq arreqeklka eerarkkeee rqrraaileq
     1381 hkvkkaieea eregkvidke llnaikptkl rnktattrpr pktihvdaga dldsgaltps
     1441 rgkkgsssnl stasltsptm rrdyyrgsqd sltaahfder rsgplyrggs lrvssvdspd
     1501 dgrgsspcrs mnqlgrrgsy ktsrdvqepq qqvrgrpkyp syqnfkgrks nslmnlcdtd
     1561 sglgratppr rapspgmgsm rhlpspsgpg slppglmtkr rvfddgssdi sstpssmmdy
     1621 ngprlykqpt tksnrgimln aveycvfpgt vnkeakrrvl deiarseskh flilfrdagc
     1681 qfralysycp dreevsklyg tgpkqvtdkm fdkffkynsg gkcfsqvhtk hltvtidaft
     1741 ihnslwqgkk vnlpnkkdmp lvi
//
LOCUS       XP_034195689            1750 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195689
VERSION     XP_034195689.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339798.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195689.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1750
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X2"
                     /calculated_mol_wt=196298
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1610..1728
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1750
                     /gene="Patronin"
                     /coded_by="XM_034339798.2:1266..6518"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg
     1441 sqdsltaahf derrsgplyr ggslrvssvd spddgrgssp crsmnqlgrr gsyktsrdvq
     1501 epqqqvrgrp kypsyqnfkg rksnslmnlc gpssdqdgmm crytdtdsgl gratpprrap
     1561 spgmgsmrhl pspsgpgslp pglmtkrrvf ddgssdisst pssmmdyngp rlykqpttks
     1621 nrgimlnave ycvfpgtvnk eakrrvldei arseskhfli lfrdagcqfr alysycpdre
     1681 evsklygtgp kqvtdkmfdk ffkynsggkc fsqvhtkhlt vtidaftihn slwqgkkvnl
     1741 pnkkdmplvi
//
LOCUS       XP_034195690            1746 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195690
VERSION     XP_034195690.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339799.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195690.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1746
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X3"
                     /calculated_mol_wt=195809
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          748..>1268
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1211..>1362
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1606..1724
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1746
                     /gene="Patronin"
                     /coded_by="XM_034339799.2:1266..6506"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtkgkvksps sstsggdspa eigpptpvts gssgdtptsv settlvtqqp
      781 sqekpqrpfs lkeisedvrd vehkwlehdg sapfietrrt pdienmdleq yhqsisqmnn
      841 slseiqadiq rlanqqnqiq qqhlmtqhqq qmqqqlqqlq slsqqhlqsf giapinplts
      901 klqdsqqqsq fylhdqpqlq rrmwgqpppt qslanemaav gyqqpmdpry gtqpttyqqd
      961 mrlyqdtrnw gthppqqkgf vlhdtpqepr ylnggdhslc nnqmshpgst ypssasifnq
     1021 tppssaspqh rnavhrisql msespepkrp tvhhipikce sptekrqvta lhapvpappv
     1081 ddmkpqnisf ignddeltqg irglhitsgs rtyripsptr psisrnsfqp hpslreatps
     1141 psgtpevtpl dptdagekgf yicfdndapk kpkptlrvkr tspkkersvs syvdnedfmv
     1201 rpespsvsav drqqkqleth rdfdrekqrq aderefqrqe mrdrelqrev erekqrerre
     1261 tsaegrqsgv gliignqlan pdpnsldeme rkkerimlls lqrrqqqeem kerkeaeaqa
     1321 rreqeklkae erarkkeeer qrraaileqh kvkkaieeae regkvidkel lnaikptklr
     1381 nktattrprp ktihvdagad ldsgaltpsr gkkgsssnls tasltsptmr rdyyrgsqds
     1441 ltaahfderr sgplyrggsl rvssvdspdd grgsspcrsm nqlgrrgsyk tsrdvqepqq
     1501 qvrgrpkyps yqnfkgrksn slmnlcgpss dqdgmmcryt dtdsglgrat pprrapspgm
     1561 gsmrhlpsps gpgslppglm tkrrvfddgs sdisstpssm mdyngprlyk qpttksnrgi
     1621 mlnaveycvf pgtvnkeakr rvldeiarse skhflilfrd agcqfralys ycpdreevsk
     1681 lygtgpkqvt dkmfdkffky nsggkcfsqv htkhltvtid aftihnslwq gkkvnlpnkk
     1741 dmplvi
//
LOCUS       XP_034195692            1746 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195692
VERSION     XP_034195692.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339801.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195692.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1746
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X4"
                     /calculated_mol_wt=195926
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1606..1724
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1746
                     /gene="Patronin"
                     /coded_by="XM_034339801.2:1266..6506"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg
     1441 sqdsltaahf derrsgplyr ggslrdspdd grgsspcrsm nqlgrrgsyk tsrdvqepqq
     1501 qvrgrpkyps yqnfkgrksn slmnlcgpss dqdgmmcryt dtdsglgrat pprrapspgm
     1561 gsmrhlpsps gpgslppglm tkrrvfddgs sdisstpssm mdyngprlyk qpttksnrgi
     1621 mlnaveycvf pgtvnkeakr rvldeiarse skhflilfrd agcqfralys ycpdreevsk
     1681 lygtgpkqvt dkmfdkffky nsggkcfsqv htkhltvtid aftihnslwq gkkvnlpnkk
     1741 dmplvi
//
LOCUS       XP_034195693            1746 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X5 [Osmia lignaria lignaria].
ACCESSION   XP_034195693
VERSION     XP_034195693.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339802.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195693.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1746
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X5"
                     /calculated_mol_wt=195860
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          <1211..>1362
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1606..1724
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1746
                     /gene="Patronin"
                     /coded_by="XM_034339802.2:1266..6506"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 hlseiqadiq rlanqqnqiq qqhlmtqhqq qmqqqlqqlq slsqqhlqsf giapinplts
      901 klqdsqqqsq fylhdqpqlq rrmwgqpppt qslanemaav gyqqpmdpry gtqpttyqqd
      961 mrlyqdtrnw gthppqqkgf vlhdtpqepr ylnggdhslc nnqmshpgst ypssasifnq
     1021 tppssaspqh rnavhrisql msespepkrp tvhhipikce sptekrqvta lhapvpappv
     1081 ddmkpqnisf ignddeltqg irglhitsgs rtyripsptr psisrnsfqp hpslreatps
     1141 psgtpevtpl dptdagekgf yicfdndapk kpkptlrvkr tspkkersvs syvdnedfmv
     1201 rpespsvsav drqqkqleth rdfdrekqrq aderefqrqe mrdrelqrev erekqrerre
     1261 tsaegrqsgv gliignqlan pdpnsldeme rkkerimlls lqrrqqqeem kerkeaeaqa
     1321 rreqeklkae erarkkeeer qrraaileqh kvkkaieeae regkvidkel lnaikptklr
     1381 nktattrprp ktihvdagad ldsgaltpsr gkkgsssnls tasltsptmr rdyyrgsqds
     1441 ltaahfderr sgplyrggsl rvssvdspdd grgsspcrsm nqlgrrgsyk tsrdvqepqq
     1501 qvrgrpkyps yqnfkgrksn slmnlcgpss dqdgmmcryt dtdsglgrat pprrapspgm
     1561 gsmrhlpsps gpgslppglm tkrrvfddgs sdisstpssm mdyngprlyk qpttksnrgi
     1621 mlnaveycvf pgtvnkeakr rvldeiarse skhflilfrd agcqfralys ycpdreevsk
     1681 lygtgpkqvt dkmfdkffky nsggkcfsqv htkhltvtid aftihnslwq gkkvnlpnkk
     1741 dmplvi
//
LOCUS       XP_034195694            1736 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195694
VERSION     XP_034195694.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339803.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195694.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1736
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X6"
                     /calculated_mol_wt=194768
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1596..1714
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1736
                     /gene="Patronin"
                     /coded_by="XM_034339803.2:1266..6476"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg
     1441 sqdsltaahf derrsgplyr ggslrvssvd spddgrgssp crsmnqlgrr gsyktsrdvq
     1501 epqqqvrgrp kypsyqnfkg rksnslmnlc dtdsglgrat pprrapspgm gsmrhlpsps
     1561 gpgslppglm tkrrvfddgs sdisstpssm mdyngprlyk qpttksnrgi mlnaveycvf
     1621 pgtvnkeakr rvldeiarse skhflilfrd agcqfralys ycpdreevsk lygtgpkqvt
     1681 dkmfdkffky nsggkcfsqv htkhltvtid aftihnslwq gkkvnlpnkk dmplvi
//
LOCUS       XP_034195695            1732 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X7 [Osmia lignaria lignaria].
ACCESSION   XP_034195695
VERSION     XP_034195695.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339804.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195695.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1732
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X7"
                     /calculated_mol_wt=194280
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          748..>1268
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1211..>1362
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1592..1710
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1732
                     /gene="Patronin"
                     /coded_by="XM_034339804.2:1266..6464"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtkgkvksps sstsggdspa eigpptpvts gssgdtptsv settlvtqqp
      781 sqekpqrpfs lkeisedvrd vehkwlehdg sapfietrrt pdienmdleq yhqsisqmnn
      841 slseiqadiq rlanqqnqiq qqhlmtqhqq qmqqqlqqlq slsqqhlqsf giapinplts
      901 klqdsqqqsq fylhdqpqlq rrmwgqpppt qslanemaav gyqqpmdpry gtqpttyqqd
      961 mrlyqdtrnw gthppqqkgf vlhdtpqepr ylnggdhslc nnqmshpgst ypssasifnq
     1021 tppssaspqh rnavhrisql msespepkrp tvhhipikce sptekrqvta lhapvpappv
     1081 ddmkpqnisf ignddeltqg irglhitsgs rtyripsptr psisrnsfqp hpslreatps
     1141 psgtpevtpl dptdagekgf yicfdndapk kpkptlrvkr tspkkersvs syvdnedfmv
     1201 rpespsvsav drqqkqleth rdfdrekqrq aderefqrqe mrdrelqrev erekqrerre
     1261 tsaegrqsgv gliignqlan pdpnsldeme rkkerimlls lqrrqqqeem kerkeaeaqa
     1321 rreqeklkae erarkkeeer qrraaileqh kvkkaieeae regkvidkel lnaikptklr
     1381 nktattrprp ktihvdagad ldsgaltpsr gkkgsssnls tasltsptmr rdyyrgsqds
     1441 ltaahfderr sgplyrggsl rvssvdspdd grgsspcrsm nqlgrrgsyk tsrdvqepqq
     1501 qvrgrpkyps yqnfkgrksn slmnlcdtds glgratpprr apspgmgsmr hlpspsgpgs
     1561 lppglmtkrr vfddgssdis stpssmmdyn gprlykqptt ksnrgimlna veycvfpgtv
     1621 nkeakrrvld eiarseskhf lilfrdagcq fralysycpd reevsklygt gpkqvtdkmf
     1681 dkffkynsgg kcfsqvhtkh ltvtidafti hnslwqgkkv nlpnkkdmpl vi
//
LOCUS       XP_076546695            1731 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X8 [Osmia lignaria lignaria].
ACCESSION   XP_076546695
VERSION     XP_076546695.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690580.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1731
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X8"
                     /calculated_mol_wt=194114
     Region          218..297
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          643..701
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          <1210..>1361
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1591..1709
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1731
                     /gene="Patronin"
                     /coded_by="XM_076690580.1:897..6092"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mtvgfkmase vmlaptaapg tsrreiddvr dmvrnatvtt iyakqrasvk wllskaynnr
       61 vpenlrepyy vdhedqehlk pqivhalsna elyclalani ysdpnyhnqn hcgilqalar
      121 kgvylaepnn tqltetiliq nsplkmsahm avieglmvly akevvtgdrv vsairrfdpq
      181 advdvptdhe kglllwisha shaliakiqt eegagdktrl pelpaakdfq slcdgvglaa
      241 vvafycpgel nwmeirvskr psiadalhnl slvhafcnrc lpysifhmqp edvtymrgsm
      301 kqnlvvflad mynvleihpa kcvrypgeer amqfldacpr nshgvahkrs lpqsiapipd
      361 lrsnlsvsap gftvakapss sssvkksqsl qqtaenyshd drragseesf vvhrgkgipt
      421 lssvadeksv tradaagrps nwedqrrssy agrrsrrnsv sddsqltien fggsqdnlhn
      481 fgrnpdkevg ahigkrstte ptlparssvq dvygsgvqhi lsdngygnee paklrrqtsn
      541 ssldnvalkq ilhssdndns egdtsklasf anlsrqssdk ginltyteqe reditsksnl
      601 snkkhgqtng ngngekkttf atlpntttwq qqsnqqsqqa eqhsvdengg ntimasqlnn
      661 irlkleekrr hienekrrme vvmskqrqkv gkaaflqavt kgkvkspsss tsggdspaei
      721 gpptpvtsgs sgdtptsvse ttlvtqqpsq ekpqrpfslk eisedvrdve hkwlehdgsa
      781 pfietrrtpd ienmdleqyh qsisqmnnsl seiqadiqrl anqqnqiqqq hlmtqhqqqm
      841 qqqlqqlqsl sqqhlqsfgi apinpltskl qdsqqqsqfy lhdqpqlqrr mwgqppptqs
      901 lanemaavgy qqpmdprygt qptstyimdq likqsldknk llgiynllyv tksiayqqdm
      961 rlyqdtrnwg thppqqkgfv lhdtpqepry lnggdhslcn nqmshpgsty pssasifnqt
     1021 ppssaspqhr navhrisqlm sespepkrpt vhhipikces ptekrqvtal hapvpappvd
     1081 dmkpqnisfi gnddeltqgi rglhitsgsr tyripsptrp sisrnsfqph pslreatpsp
     1141 sgtpevtpld ptdagekgfy icfdndapkk pkptlrvkrt spkkersvss yvdnedfmvr
     1201 pespsvsavd rqqkqlethr dfdrekqrqa derefqrqem rdrelqreve rekqrerret
     1261 saegrqsgvg liignqlanp dpnsldemer kkerimllsl qrrqqqeemk erkeaeaqar
     1321 reqeklkaee rarkkeeerq rraaileqhk vkkaieeaer egkvidkell naikptklrn
     1381 ktattrprpk tihvdagadl dsgaltpsrg kkgsssnlst asltsptmrr dyyrgsqdsl
     1441 taahfderrs gplyrggslr vssvdspddg rgsspcrsmn qlgrrgsykt srdvqepqqq
     1501 vrgrpkypsy qnfkgrksns lmnlcdtdsg lgratpprra pspgmgsmrh lpspsgpgsl
     1561 ppglmtkrrv fddgssdiss tpssmmdyng prlykqpttk snrgimlnav eycvfpgtvn
     1621 keakrrvlde iarseskhfl ilfrdagcqf ralysycpdr eevsklygtg pkqvtdkmfd
     1681 kffkynsggk cfsqvhtkhl tvtidaftih nslwqgkkvn lpnkkdmplv i
//
LOCUS       XP_076546696            1726 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X9 [Osmia lignaria lignaria].
ACCESSION   XP_076546696
VERSION     XP_076546696.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690581.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1726
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X9"
                     /calculated_mol_wt=193984
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          638..696
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          <1205..>1356
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1586..1704
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1726
                     /gene="Patronin"
                     /coded_by="XM_076690581.1:1266..6446"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldva kapsssssvk ksqslqqtae nyshddrrag seesfvvhrg kgiptlssva
      421 deksvtrada agrpsnwedq rrssyagrrs rrnsvsddsq ltienfggsq dnlhnfgrnp
      481 dkevgahigk rstteptlpa rssvqdvygs gvqhilsdng ygneepaklr rqtsnssldn
      541 valkqilhss dndnsegdts klasfanlsr qssdkginlt yteqeredit sksnlsnkkh
      601 gqtngngnge kkttfatlpn tttwqqqsnq qsqqaeqhsv denggntima sqlnnirlkl
      661 eekrrhiene krrmevvmsk qrqkvgkaaf lqavtkgkvk spssstsggd spaeigpptp
      721 vtsgssgdtp tsvsettlvt qqpsqekpqr pfslkeised vrdvehkwle hdgsapfiet
      781 rrtpdienmd leqyhqsisq mnnslseiqa diqrlanqqn qiqqqhlmtq hqqqmqqqlq
      841 qlqslsqqhl qsfgiapinp ltsklqdsqq qsqfylhdqp qlqrrmwgqp pptqslanem
      901 aavgyqqpmd prygtqptst yimdqlikqs ldknkllgiy nllyvtksia yqqdmrlyqd
      961 trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas ifnqtppssa
     1021 spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp appvddmkpq
     1081 nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre atpspsgtpe
     1141 vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne dfmvrpesps
     1201 vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr erretsaegr
     1261 qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea eaqarreqek
     1321 lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp tklrnktatt
     1381 rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg sqdsltaahf
     1441 derrsgplyr ggslrvssvd spddgrgssp crsmnqlgrr gsyktsrdvq epqqqvrgrp
     1501 kypsyqnfkg rksnslmnlc dtdsglgrat pprrapspgm gsmrhlpsps gpgslppglm
     1561 tkrrvfddgs sdisstpssm mdyngprlyk qpttksnrgi mlnaveycvf pgtvnkeakr
     1621 rvldeiarse skhflilfrd agcqfralys ycpdreevsk lygtgpkqvt dkmfdkffky
     1681 nsggkcfsqv htkhltvtid aftihnslwq gkkvnlpnkk dmplvi
//
LOCUS       XP_034195697            1717 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X10 [Osmia lignaria lignaria].
ACCESSION   XP_034195697
VERSION     XP_034195697.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339806.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195697.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1717
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X10"
                     /calculated_mol_wt=192419
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1577..1695
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1717
                     /gene="Patronin"
                     /coded_by="XM_034339806.2:1266..6419"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg
     1441 sqdsltaahf derrsgplyr ggslrvssvd spddgrgssp crsmnqlgrr gsyktsrgps
     1501 sdqdgmmcry tdtdsglgra tpprrapspg mgsmrhlpsp sgpgslppgl mtkrrvfddg
     1561 ssdisstpss mmdyngprly kqpttksnrg imlnaveycv fpgtvnkeak rrvldeiars
     1621 eskhflilfr dagcqfraly sycpdreevs klygtgpkqv tdkmfdkffk ynsggkcfsq
     1681 vhtkhltvti daftihnslw qgkkvnlpnk kdmplvi
//
LOCUS       XP_034195699            1713 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X11 [Osmia lignaria lignaria].
ACCESSION   XP_034195699
VERSION     XP_034195699.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339808.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195699.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1713
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X11"
                     /calculated_mol_wt=192046
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1573..1691
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1713
                     /gene="Patronin"
                     /coded_by="XM_034339808.2:1266..6407"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg
     1441 sqdsltaahf derrsgplyr ggslrdspdd grgsspcrsm nqlgrrgsyk tsrgpssdqd
     1501 gmmcrytdtd sglgratppr rapspgmgsm rhlpspsgpg slppglmtkr rvfddgssdi
     1561 sstpssmmdy ngprlykqpt tksnrgimln aveycvfpgt vnkeakrrvl deiarseskh
     1621 flilfrdagc qfralysycp dreevsklyg tgpkqvtdkm fdkffkynsg gkcfsqvhtk
     1681 hltvtidaft ihnslwqgkk vnlpnkkdmp lvi
//
LOCUS       XP_034195700            1710 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X12 [Osmia lignaria lignaria].
ACCESSION   XP_034195700
VERSION     XP_034195700.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339809.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195700.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1710
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X12"
                     /calculated_mol_wt=191783
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1570..1688
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1710
                     /gene="Patronin"
                     /coded_by="XM_034339809.2:1266..6398"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstvssvd spddgrgssp
     1441 crsmnqlgrr gsyktsrdvq epqqqvrgrp kypsyqnfkg rksnslmnlc gpssdqdgmm
     1501 crytdtdsgl gratpprrap spgmgsmrhl pspsgpgslp pglmtkrrvf ddgssdisst
     1561 pssmmdyngp rlykqpttks nrgimlnave ycvfpgtvnk eakrrvldei arseskhfli
     1621 lfrdagcqfr alysycpdre evsklygtgp kqvtdkmfdk ffkynsggkc fsqvhtkhlt
     1681 vtidaftihn slwqgkkvnl pnkkdmplvi
//
LOCUS       XP_034195701            1706 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X13 [Osmia lignaria lignaria].
ACCESSION   XP_034195701
VERSION     XP_034195701.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339810.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195701.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1706
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X13"
                     /calculated_mol_wt=191411
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1566..1684
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1706
                     /gene="Patronin"
                     /coded_by="XM_034339810.2:1266..6386"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstdspdd grgsspcrsm
     1441 nqlgrrgsyk tsrdvqepqq qvrgrpkyps yqnfkgrksn slmnlcgpss dqdgmmcryt
     1501 dtdsglgrat pprrapspgm gsmrhlpsps gpgslppglm tkrrvfddgs sdisstpssm
     1561 mdyngprlyk qpttksnrgi mlnaveycvf pgtvnkeakr rvldeiarse skhflilfrd
     1621 agcqfralys ycpdreevsk lygtgpkqvt dkmfdkffky nsggkcfsqv htkhltvtid
     1681 aftihnslwq gkkvnlpnkk dmplvi
//
LOCUS       XP_034195703            1703 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X14 [Osmia lignaria lignaria].
ACCESSION   XP_034195703
VERSION     XP_034195703.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339812.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195703.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1703
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X14"
                     /calculated_mol_wt=190889
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1563..1681
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1703
                     /gene="Patronin"
                     /coded_by="XM_034339812.2:1266..6377"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg
     1441 sqdsltaahf derrsgplyr ggslrvssvd spddgrgssp crsmnqlgrr gsyktsrdtd
     1501 sglgratppr rapspgmgsm rhlpspsgpg slppglmtkr rvfddgssdi sstpssmmdy
     1561 ngprlykqpt tksnrgimln aveycvfpgt vnkeakrrvl deiarseskh flilfrdagc
     1621 qfralysycp dreevsklyg tgpkqvtdkm fdkffkynsg gkcfsqvhtk hltvtidaft
     1681 ihnslwqgkk vnlpnkkdmp lvi
//
LOCUS       XP_034195704            1699 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X15 [Osmia lignaria lignaria].
ACCESSION   XP_034195704
VERSION     XP_034195704.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339813.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195704.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1699
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X15"
                     /calculated_mol_wt=190516
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1559..1677
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1699
                     /gene="Patronin"
                     /coded_by="XM_034339813.2:1266..6365"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstaslts ptmrrdyyrg
     1441 sqdsltaahf derrsgplyr ggslrdspdd grgsspcrsm nqlgrrgsyk tsrdtdsglg
     1501 ratpprraps pgmgsmrhlp spsgpgslpp glmtkrrvfd dgssdisstp ssmmdyngpr
     1561 lykqpttksn rgimlnavey cvfpgtvnke akrrvldeia rseskhflil frdagcqfra
     1621 lysycpdree vsklygtgpk qvtdkmfdkf fkynsggkcf sqvhtkhltv tidaftihns
     1681 lwqgkkvnlp nkkdmplvi
//
LOCUS       XP_034195705            1687 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X16 [Osmia lignaria lignaria].
ACCESSION   XP_034195705
VERSION     XP_034195705.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339814.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195705.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1687
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X16"
                     /calculated_mol_wt=189899
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          <835..>1209
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1152..>1303
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1547..1665
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1687
                     /gene="Patronin"
                     /coded_by="XM_034339814.2:1266..6329"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtkeisedvr dvehkwlehd gsapfietrr tpdienmdle qyhqsisqmn
      781 nslseiqadi qrlanqqnqi qqqhlmtqhq qqmqqqlqql qslsqqhlqs fgiapinplt
      841 sklqdsqqqs qfylhdqpql qrrmwgqppp tqslanemaa vgyqqpmdpr ygtqpttyqq
      901 dmrlyqdtrn wgthppqqkg fvlhdtpqep rylnggdhsl cnnqmshpgs typssasifn
      961 qtppssaspq hrnavhrisq lmsespepkr ptvhhipikc esptekrqvt alhapvpapp
     1021 vddmkpqnis fignddeltq girglhitsg srtyripspt rpsisrnsfq phpslreatp
     1081 spsgtpevtp ldptdagekg fyicfdndap kkpkptlrvk rtspkkersv ssyvdnedfm
     1141 vrpespsvsa vdrqqkqlet hrdfdrekqr qaderefqrq emrdrelqre verekqrerr
     1201 etsaegrqsg vgliignqla npdpnsldem erkkerimll slqrrqqqee mkerkeaeaq
     1261 arreqeklka eerarkkeee rqrraaileq hkvkkaieea eregkvidke llnaikptkl
     1321 rnktattrpr pktihvdaga dldsgaltps rgkkgsssnl stasltsptm rrdyyrgsqd
     1381 sltaahfder rsgplyrggs lrvssvdspd dgrgsspcrs mnqlgrrgsy ktsrdvqepq
     1441 qqvrgrpkyp syqnfkgrks nslmnlcgps sdqdgmmcry tdtdsglgra tpprrapspg
     1501 mgsmrhlpsp sgpgslppgl mtkrrvfddg ssdisstpss mmdyngprly kqpttksnrg
     1561 imlnaveycv fpgtvnkeak rrvldeiars eskhflilfr dagcqfraly sycpdreevs
     1621 klygtgpkqv tdkmfdkffk ynsggkcfsq vhtkhltvti daftihnslw qgkkvnlpnk
     1681 kdmplvi
//
LOCUS       XP_034195706            1677 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X17 [Osmia lignaria lignaria].
ACCESSION   XP_034195706
VERSION     XP_034195706.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339815.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195706.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1677
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X17"
                     /calculated_mol_wt=187904
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1537..1655
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1677
                     /gene="Patronin"
                     /coded_by="XM_034339815.2:1266..6299"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstvssvd spddgrgssp
     1441 crsmnqlgrr gsyktsrgps sdqdgmmcry tdtdsglgra tpprrapspg mgsmrhlpsp
     1501 sgpgslppgl mtkrrvfddg ssdisstpss mmdyngprly kqpttksnrg imlnaveycv
     1561 fpgtvnkeak rrvldeiars eskhflilfr dagcqfraly sycpdreevs klygtgpkqv
     1621 tdkmfdkffk ynsggkcfsq vhtkhltvti daftihnslw qgkkvnlpnk kdmplvi
//
LOCUS       XP_034195707            1673 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X18 [Osmia lignaria lignaria].
ACCESSION   XP_034195707
VERSION     XP_034195707.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339816.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195707.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1673
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X18"
                     /calculated_mol_wt=187531
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1533..1651
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1673
                     /gene="Patronin"
                     /coded_by="XM_034339816.2:1266..6287"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstdspdd grgsspcrsm
     1441 nqlgrrgsyk tsrgpssdqd gmmcrytdtd sglgratppr rapspgmgsm rhlpspsgpg
     1501 slppglmtkr rvfddgssdi sstpssmmdy ngprlykqpt tksnrgimln aveycvfpgt
     1561 vnkeakrrvl deiarseskh flilfrdagc qfralysycp dreevsklyg tgpkqvtdkm
     1621 fdkffkynsg gkcfsqvhtk hltvtidaft ihnslwqgkk vnlpnkkdmp lvi
//
LOCUS       XP_034195708            1663 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X19 [Osmia lignaria lignaria].
ACCESSION   XP_034195708
VERSION     XP_034195708.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339817.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195708.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1663
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X19"
                     /calculated_mol_wt=186374
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1523..1641
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1663
                     /gene="Patronin"
                     /coded_by="XM_034339817.2:1266..6257"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstvssvd spddgrgssp
     1441 crsmnqlgrr gsyktsrdtd sglgratppr rapspgmgsm rhlpspsgpg slppglmtkr
     1501 rvfddgssdi sstpssmmdy ngprlykqpt tksnrgimln aveycvfpgt vnkeakrrvl
     1561 deiarseskh flilfrdagc qfralysycp dreevsklyg tgpkqvtdkm fdkffkynsg
     1621 gkcfsqvhtk hltvtidaft ihnslwqgkk vnlpnkkdmp lvi
//
LOCUS       XP_034195709            1659 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X20 [Osmia lignaria lignaria].
ACCESSION   XP_034195709
VERSION     XP_034195709.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339818.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195709.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1659
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X20"
                     /calculated_mol_wt=186001
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1519..1637
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1659
                     /gene="Patronin"
                     /coded_by="XM_034339818.2:1266..6245"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstdspdd grgsspcrsm
     1441 nqlgrrgsyk tsrdtdsglg ratpprraps pgmgsmrhlp spsgpgslpp glmtkrrvfd
     1501 dgssdisstp ssmmdyngpr lykqpttksn rgimlnavey cvfpgtvnke akrrvldeia
     1561 rseskhflil frdagcqfra lysycpdree vsklygtgpk qvtdkmfdkf fkynsggkcf
     1621 sqvhtkhltv tidaftihns lwqgkkvnlp nkkdmplvi
//
LOCUS       XP_034195710            1645 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X21 [Osmia lignaria lignaria].
ACCESSION   XP_034195710
VERSION     XP_034195710.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339819.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195710.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1645
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X21"
                     /calculated_mol_wt=184478
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1505..1623
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1645
                     /gene="Patronin"
                     /coded_by="XM_034339819.2:1266..6203"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstgpssd qdgmmcrytd
     1441 tdsglgratp prrapspgmg smrhlpspsg pgslppglmt krrvfddgss disstpssmm
     1501 dyngprlykq pttksnrgim lnaveycvfp gtvnkeakrr vldeiarses khflilfrda
     1561 gcqfralysy cpdreevskl ygtgpkqvtd kmfdkffkyn sggkcfsqvh tkhltvtida
     1621 ftihnslwqg kkvnlpnkkd mplvi
//
LOCUS       XP_034195711            1631 aa            linear   INV 03-SEP-2025
DEFINITION  calmodulin-regulated spectrin-associated protein patronin isoform
            X22 [Osmia lignaria lignaria].
ACCESSION   XP_034195711
VERSION     XP_034195711.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339820.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195711.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1631
                     /product="calmodulin-regulated spectrin-associated protein
                     patronin isoform X22"
                     /calculated_mol_wt=182948
     Region          250..329
                     /region_name="CH_SF"
                     /note="calponin homology (CH) domain superfamily; cl00030"
                     /db_xref="CDD:469584"
     Region          675..733
                     /region_name="CAMSAP_CC1"
                     /note="Spectrin-binding region of Ca2+-Calmodulin;
                     pfam17095"
                     /db_xref="CDD:465344"
     Region          752..>1272
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <1215..>1366
                     /region_name="TPH"
                     /note="Trichohyalin-plectin-homology domain; pfam13868"
                     /db_xref="CDD:464007"
     Region          1491..1609
                     /region_name="CAMSAP_CKK"
                     /note="Microtubule-binding calmodulin-regulated
                     spectrin-associated; pfam08683"
                     /db_xref="CDD:462558"
     CDS             1..1631
                     /gene="Patronin"
                     /coded_by="XM_034339820.2:1266..6161"
                     /db_xref="GeneID:117611700"
ORIGIN      
        1 mwsaisrlfv kgkteepapr tedwtcddvp dtvvhvfdam drnagddrrk gpageqqhgg
       61 tesehfsday dsrqakqras vkwllskayn nrvpenlrep yyvdhedqeh lkpqivhals
      121 naelyclala niysdpnyhn qnhcgilqal arkgvylaep nntqltetil iqnsplkmsa
      181 hmavieglmv lyakevvtgd rvvsairrfd pqadvdvptd hekglllwis hashaliaki
      241 qteegagdkt rlpelpaakd fqslcdgvgl aavvafycpg elnwmeirvs krpsiadalh
      301 nlslvhafcn rclpysifhm qpedvtymrg smkqnlvvfl admynvleih pakcvrypge
      361 eramqfldac prnshgvahk rslpqsiapi pdlrsnlsvs apgftvakap sssssvkksq
      421 slqqtaenys hddrragsee sfvvhrgkgi ptlssvadek svtradaagr psnwedqrrs
      481 syagrrsrrn svsddsqlti enfggsqdnl hnfgrnpdke vgahigkrst teptlparss
      541 vqdvygsgvq hilsdngygn eepaklrrqt snssldnval kqilhssdnd nsegdtskla
      601 sfanlsrqss dkginltyte qereditsks nlsnkkhgqt ngngngekkt tfatlpnttt
      661 wqqqsnqqsq qaeqhsvden ggntimasql nnirlkleek rrhienekrr mevvmskqrq
      721 kvgkaaflqa vtklylvgkv kspssstsgg dspaeigppt pvtsgssgdt ptsvsettlv
      781 tqqpsqekpq rpfslkeise dvrdvehkwl ehdgsapfie trrtpdienm dleqyhqsis
      841 qmnnslseiq adiqrlanqq nqiqqqhlmt qhqqqmqqql qqlqslsqqh lqsfgiapin
      901 pltsklqdsq qqsqfylhdq pqlqrrmwgq ppptqslane maavgyqqpm dprygtqptt
      961 yqqdmrlyqd trnwgthppq qkgfvlhdtp qeprylnggd hslcnnqmsh pgstypssas
     1021 ifnqtppssa spqhrnavhr isqlmsespe pkrptvhhip ikcesptekr qvtalhapvp
     1081 appvddmkpq nisfigndde ltqgirglhi tsgsrtyrip sptrpsisrn sfqphpslre
     1141 atpspsgtpe vtpldptdag ekgfyicfdn dapkkpkptl rvkrtspkke rsvssyvdne
     1201 dfmvrpesps vsavdrqqkq lethrdfdre kqrqaderef qrqemrdrel qreverekqr
     1261 erretsaegr qsgvgliign qlanpdpnsl demerkkeri mllslqrrqq qeemkerkea
     1321 eaqarreqek lkaeerarkk eeerqrraai leqhkvkkai eeaeregkvi dkellnaikp
     1381 tklrnktatt rprpktihvd agadldsgal tpsrgkkgss snlstdtdsg lgratpprra
     1441 pspgmgsmrh lpspsgpgsl ppglmtkrrv fddgssdiss tpssmmdyng prlykqpttk
     1501 snrgimlnav eycvfpgtvn keakrrvlde iarseskhfl ilfrdagcqf ralysycpdr
     1561 eevsklygtg pkqvtdkmfd kffkynsggk cfsqvhtkhl tvtidaftih nslwqgkkvn
     1621 lpnkkdmplv i
//
LOCUS       XP_034195741             520 aa            linear   INV 03-SEP-2025
DEFINITION  protein SHQ1 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195741
VERSION     XP_034195741.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339850.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195741.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..520
                     /product="protein SHQ1 homolog isoform X1"
                     /calculated_mol_wt=59605
     Region          232..418
                     /region_name="SHQ1"
                     /note="SHQ1 protein; pfam04925"
                     /db_xref="CDD:461483"
     CDS             1..520
                     /gene="LOC117611708"
                     /coded_by="XM_034339850.2:170..1732"
                     /db_xref="GeneID:117611708"
ORIGIN      
        1 mltprfeitq tdaevaimih apyanikdte vyvegtdfrf ystpyylrlr lpreieends
       61 sfgsydcekg dfslrfskvn hgeyfenldm istllapqkk kstivpniev ignpsansed
      121 ineisnehns tlneningde wyipqnnpte tipllssspk ygfankisaa lvafesawik
      181 evidlpmpdt tlqserkilr ekrelsdfne ehyladlmqp eciepyllft aewdtleken
      241 isfneseidv lkelpnkeyl ldneetdrll lslvdilfgs cynhrttlge ntvesswtin
      301 klsstlswfd dfstpeevik acfrrsicyp lfrnwklsik vledvkkvvk lgkkyiikrf
      361 ceihgmfnns cepryilnql yikdyliwlq qinesliell dtlianvqps keemgldlie
      421 leqaaysvqe edliiensvh evvdqmdelt vndndknkpk tiyyikdkhs lkyllsssss
      481 ssdssdtdse sdsettsstt ttsdssslds ddtsepegct
//
LOCUS       XP_034195742             504 aa            linear   INV 03-SEP-2025
DEFINITION  protein SHQ1 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195742
VERSION     XP_034195742.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339851.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195742.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..504
                     /product="protein SHQ1 homolog isoform X2"
                     /calculated_mol_wt=57612
     Region          232..418
                     /region_name="SHQ1"
                     /note="SHQ1 protein; pfam04925"
                     /db_xref="CDD:461483"
     CDS             1..504
                     /gene="LOC117611708"
                     /coded_by="XM_034339851.2:170..1684"
                     /db_xref="GeneID:117611708"
ORIGIN      
        1 mltprfeitq tdaevaimih apyanikdte vyvegtdfrf ystpyylrlr lpreieends
       61 sfgsydcekg dfslrfskvn hgeyfenldm istllapqkk kstivpniev ignpsansed
      121 ineisnehns tlneningde wyipqnnpte tipllssspk ygfankisaa lvafesawik
      181 evidlpmpdt tlqserkilr ekrelsdfne ehyladlmqp eciepyllft aewdtleken
      241 isfneseidv lkelpnkeyl ldneetdrll lslvdilfgs cynhrttlge ntvesswtin
      301 klsstlswfd dfstpeevik acfrrsicyp lfrnwklsik vledvkkvvk lgkkyiikrf
      361 ceihgmfnns cepryilnql yikdyliwlq qinesliell dtlianvqps keemgldlie
      421 leqaaysvqe edliiensvh evvdqmdelt vndndknkls ssssssdssd tdsesdsett
      481 ssttttsdss sldsddtsep egct
//
LOCUS       XP_034195743             503 aa            linear   INV 03-SEP-2025
DEFINITION  protein SHQ1 homolog isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195743
VERSION     XP_034195743.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339852.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195743.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..503
                     /product="protein SHQ1 homolog isoform X3"
                     /calculated_mol_wt=57688
     Region          215..401
                     /region_name="SHQ1"
                     /note="SHQ1 protein; pfam04925"
                     /db_xref="CDD:461483"
     CDS             1..503
                     /gene="LOC117611708"
                     /coded_by="XM_034339852.2:369..1880"
                     /db_xref="GeneID:117611708"
ORIGIN      
        1 mihapyanik dtevyvegtd frfystpyyl rlrlpreiee ndssfgsydc ekgdfslrfs
       61 kvnhgeyfen ldmistllap qkkkstivpn ievignpsan sedineisne hnstlnenin
      121 gdewyipqnn ptetipllss spkygfanki saalvafesa wikevidlpm pdttlqserk
      181 ilrekrelsd fneehyladl mqpeciepyl lftaewdtle kenisfnese idvlkelpnk
      241 eylldneetd rlllslvdil fgscynhrtt lgentvessw tinklsstls wfddfstpee
      301 vikacfrrsi cyplfrnwkl sikvledvkk vvklgkkyii krfceihgmf nnscepryil
      361 nqlyikdyli wlqqinesli elldtlianv qpskeemgld lieleqaays vqeedliien
      421 svhevvdqmd eltvndndkn kpktiyyikd khslkyllss sssssdssdt dsesdsetts
      481 sttttsdsss ldsddtsepe gct
//
LOCUS       XP_034195749             439 aa            linear   INV 03-SEP-2025
DEFINITION  glass bottom boat isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195749
VERSION     XP_034195749.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339858.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..439
                     /product="glass bottom boat isoform X1"
                     /calculated_mol_wt=49671
     Region          51..291
                     /region_name="TGFb_propeptide"
                     /note="TGF-beta propeptide; pfam00688"
                     /db_xref="CDD:459906"
     Region          338..439
                     /region_name="TGF_beta_BMP5_like"
                     /note="transforming growth factor beta (TGF-beta) like
                     domain found in bone morphogenetic proteins BMP-5, BMP-6,
                     BMP-7, BMP-8A/B and similar proteins; cd13761"
                     /db_xref="CDD:381639"
     Site            order(343,345,349..350,352,362,364..366,368,383..384,
                     387..388,390,394,400,403..406,409,429..431,433,439)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:381639"
     CDS             1..439
                     /gene="gbb"
                     /coded_by="XM_034339858.2:760..2079"
                     /db_xref="GeneID:117611711"
ORIGIN      
        1 mvpsgpcrsr aerraemspr rlapllilgt ilagltstaa aermsglyvd ngfdqtivhr
       61 vvsqrekrev eheilnllgl pdrprntagr ppqvkrsapk flldiyknal gededekpia
      121 eqrsvsefdl tgqdlraidq sdvimtfaah nhhvpgvrhe rgkrlwfdvs evppgehiig
      181 aelrlyrsmd vknrrnrgsy mitayrvlrt edgtrelqyv davntttgke gwltlnvsep
      241 ldhwvnnpdg nkglylsvhp adrsvhemrp edigivgfrg dpdkqpfmvg yfkssgiret
      301 kvrqkrdarr rkksesssld yrnnpytdpg vqynsrtcki qtlyvsfrdl kwqdwiiapd
      361 gydayycsge cnfplnahmn atnhaivqtl vhlvspgkvp kpccaptkls pisvlyflde
      421 snvilkkykn mvvkscgch
//
LOCUS       XP_034195750             354 aa            linear   INV 03-SEP-2025
DEFINITION  glass bottom boat isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195750
VERSION     XP_034195750.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339859.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..354
                     /product="glass bottom boat isoform X2"
                     /calculated_mol_wt=39857
     Region          51..291
                     /region_name="TGFb_propeptide"
                     /note="TGF-beta propeptide; pfam00688"
                     /db_xref="CDD:459906"
     CDS             1..354
                     /gene="gbb"
                     /coded_by="XM_034339859.2:765..1829"
                     /db_xref="GeneID:117611711"
ORIGIN      
        1 mvpsgpcrsr aerraemspr rlapllilgt ilagltstaa aermsglyvd ngfdqtivhr
       61 vvsqrekrev eheilnllgl pdrprntagr ppqvkrsapk flldiyknal gededekpia
      121 eqrsvsefdl tgqdlraidq sdvimtfaah nhhvpgvrhe rgkrlwfdvs evppgehiig
      181 aelrlyrsmd vknrrnrgsy mitayrvlrt edgtrelqyv davntttgke gwltlnvsep
      241 ldhwvnnpdg nkglylsvhp adrsvhemrp edigivgfrg dpdkqpfmvg yfkssgiret
      301 kvrqkrdarr rkksesssld yrnnpytgkf tkilvcsttl gpvkskrcts vsgt
//
LOCUS       XP_034195756             226 aa            linear   INV 03-SEP-2025
DEFINITION  spliceosome-associated protein CWC15 [Osmia lignaria lignaria].
ACCESSION   XP_034195756
VERSION     XP_034195756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..226
                     /product="spliceosome-associated protein CWC15"
                     /calculated_mol_wt=26304
     Region          1..225
                     /region_name="Cwf_Cwc_15"
                     /note="Cwf15/Cwc15 cell cycle control protein; pfam04889"
                     /db_xref="CDD:428176"
     CDS             1..226
                     /gene="c12.1"
                     /coded_by="XM_034339865.2:149..829"
                     /db_xref="GeneID:117611716"
ORIGIN      
        1 mttaarptfe parggqgrge kdlsaiskqy ssrdlpshtk lkyrehgqgt ieelrnrdfr
       61 keleererer ekekssnrrm ieapretsts sakrqkldqv ptasldaddp lddddseses
      121 deddtaalla elqrikkera aeqakkemek rqeeerirme nilsgnplln ysaqsartdm
      181 kvrrrwdddv vfkncarsep kkkhdvfind slrsefhrkf mekyvk
//
LOCUS       XP_034195757             155 aa            linear   INV 03-SEP-2025
DEFINITION  deoxycytidylate deaminase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195757
VERSION     XP_034195757.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339866.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="deoxycytidylate deaminase-like isoform X1"
                     /calculated_mol_wt=17362
     Region          5..151
                     /region_name="ComEB"
                     /note="Deoxycytidylate deaminase [Nucleotide transport and
                     metabolism]; COG2131"
                     /db_xref="CDD:441734"
     CDS             1..155
                     /gene="LOC117611717"
                     /coded_by="XM_034339866.2:108..575"
                     /db_xref="GeneID:117611717"
ORIGIN      
        1 malahpegri etdeifmgia llasklsndk nckvgacivh nektivgvgy nampdyrdse
       61 fpfkknkhnf leckltyvch aelnaivnkt ccnirnstiy vtrhpcneca klivqfgiks
      121 vvykdnpheg mndvlaarri fdttgvtvrq yiprn
//
LOCUS       XP_034195758             151 aa            linear   INV 03-SEP-2025
DEFINITION  deoxycytidylate deaminase-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195758
VERSION     XP_034195758.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339867.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="deoxycytidylate deaminase-like isoform X2"
                     /calculated_mol_wt=16734
     Region          5..149
                     /region_name="ComEB"
                     /note="Deoxycytidylate deaminase [Nucleotide transport and
                     metabolism]; COG2131"
                     /db_xref="CDD:441734"
     CDS             1..151
                     /gene="LOC117611717"
                     /coded_by="XM_034339867.2:108..563"
                     /db_xref="GeneID:117611717"
ORIGIN      
        1 malahpegri etdeifmgia llasklsndk nckvgacivh nektivgvgy nampdyrdse
       61 fpfkknkhnf leckltyvch aelnaivnkt ccnirnstiy vtrhpcneca klivqfgiks
      121 vvykdnpheg mndvlaarri fdttgvtvrg s
//
LOCUS       XP_034195759             139 aa            linear   INV 03-SEP-2025
DEFINITION  deoxycytidylate deaminase-like isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195759
VERSION     XP_034195759.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339868.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..139
                     /product="deoxycytidylate deaminase-like isoform X3"
                     /calculated_mol_wt=15551
     Region          1..135
                     /region_name="ComEB"
                     /note="Deoxycytidylate deaminase [Nucleotide transport and
                     metabolism]; COG2131"
                     /db_xref="CDD:441734"
     CDS             1..139
                     /gene="LOC117611717"
                     /coded_by="XM_034339868.2:284..703"
                     /db_xref="GeneID:117611717"
ORIGIN      
        1 mgiallaskl sndknckvga civhnektiv gvgynampdy rdsefpfkkn khnflecklt
       61 yvchaelnai vnktccnirn stiyvtrhpc necaklivqf giksvvykdn phegmndvla
      121 arrifdttgv tvrqyiprn
//
LOCUS       XP_034195760             139 aa            linear   INV 03-SEP-2025
DEFINITION  deoxycytidylate deaminase-like isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195760
VERSION     XP_034195760.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339869.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..139
                     /product="deoxycytidylate deaminase-like isoform X3"
                     /calculated_mol_wt=15551
     Region          1..135
                     /region_name="ComEB"
                     /note="Deoxycytidylate deaminase [Nucleotide transport and
                     metabolism]; COG2131"
                     /db_xref="CDD:441734"
     CDS             1..139
                     /gene="LOC117611717"
                     /coded_by="XM_034339869.2:199..618"
                     /db_xref="GeneID:117611717"
ORIGIN      
        1 mgiallaskl sndknckvga civhnektiv gvgynampdy rdsefpfkkn khnflecklt
       61 yvchaelnai vnktccnirn stiyvtrhpc necaklivqf giksvvykdn phegmndvla
      121 arrifdttgv tvrqyiprn
//
LOCUS       XP_034195761             144 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611718 [Osmia lignaria lignaria].
ACCESSION   XP_034195761
VERSION     XP_034195761.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339870.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195761.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..144
                     /product="uncharacterized protein LOC117611718"
                     /calculated_mol_wt=17181
     CDS             1..144
                     /gene="LOC117611718"
                     /coded_by="XM_034339870.2:67..501"
                     /db_xref="GeneID:117611718"
ORIGIN      
        1 mgdlkneelr qanyellalf ddssmlshfe ncaldlsyfn ffedlrvadi dhqkeetken
       61 esrlnndtng knhvfavpkm yncmycfkqf ysrtasrrhq vchakqqlqc sycktrsysi
      121 salrrhfeyf hpctrrrqil iesr
//
LOCUS       XP_076546697             330 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305765 [Osmia lignaria lignaria].
ACCESSION   XP_076546697
VERSION     XP_076546697.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690582.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="uncharacterized protein LOC143305765"
                     /calculated_mol_wt=37364
     Region          147..276
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(150..153,182..184,187,211,255,273)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..330
                     /gene="LOC143305765"
                     /coded_by="XM_076690582.1:1..993"
                     /db_xref="GeneID:143305765"
ORIGIN      
        1 mdalrlamgy rrstptnvil getkllsikd rtsylgskyv lkaltnvtnp fakvladfce
       61 vywnqehisn peldkdrsla rvvnlieeft pllvrlpnlv antadygtqy iqpkietefi
      121 tksrntshpi eifhetmqnk yqnhkliftn gskldsspsv gaacvvpqdn yigkitlpkq
      181 asvytaecva memateyinr nyqqsyeict dslnlvqtlv spginpqink hiinikstlr
      241 qikvkapnqe vvvmwapahr giggneladr qaktaaqave kqlcqvpyid ifpvlkqkir
      301 vntenelarq erfkgreffr kfhvksnahp
//
LOCUS       XP_034194193             586 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610666 [Osmia lignaria lignaria].
ACCESSION   XP_034194193
VERSION     XP_034194193.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338302.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194193.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="uncharacterized protein LOC117610666"
                     /calculated_mol_wt=66020
     Region          13..248
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(98..103,155..156,184,186..187)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(98..103,155,186)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            156
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          295..426
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(299..302,331..333,336,360,405,423)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..586
                     /gene="LOC117610666"
                     /coded_by="XM_034338302.2:1..1761"
                     /db_xref="GeneID:117610666"
ORIGIN      
        1 mygtnnypee wkksyihfin kpdgksvrpi sltsclcklf eslvknkldw wiehnnflpr
       61 aqsgfrkghs tadnlinlti sieeassnkk dllavfldin safdnvnidi llsqlakvgc
      121 paslvkfiqh ltferlifte atginprkvy kgvpqggvls pilfniyvst iaenipkgvt
      181 iskfaddiav ysknkkllek tisriydkll nlglellthk ttfvhfnkhn ikpgqteiti
      241 kdtviksnyy pihrtdyyal ikninintef gktlvrtgnp ntlidqltte enshaiytdg
      301 skskespsvg sacmckdlni lttrsihkds siftaecvai sdamdialkh pnrkfliftd
      361 slsvllalth ptasiktnpy ileikrkise lcvkaqnsnp iklywipghk girgneeadq
      421 lakaaanlap tdrtkipftd lskqaktiat nttrdiitql gltkgknyft hfhnnhakpw
      481 ftgrnlkret itvvnrcrag hynlahfltr inivndpkcq cgyerqdigh vfwqcslldd
      541 qrkdlicklq klsyhlplsc nillqnptta icniiqsflk scnlsi
//
LOCUS       XP_076546698             144 aa            linear   INV 03-SEP-2025
DEFINITION  protein GVQW3-like [Osmia lignaria lignaria].
ACCESSION   XP_076546698
VERSION     XP_076546698.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690583.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..144
                     /product="protein GVQW3-like"
                     /calculated_mol_wt=16901
     Region          12..76
                     /region_name="HTH_32"
                     /note="Homeodomain-like domain; pfam13565"
                     /db_xref="CDD:463923"
     CDS             1..144
                     /gene="LOC143305766"
                     /coded_by="XM_076690583.1:1..435"
                     /db_xref="GeneID:143305766"
ORIGIN      
        1 mvnrayqkrk tyewykafkk greevqdspr sgrpsmvstd envekikkmv idnrrlsvre
       61 vsrdvemshm svhniltevl gmrrvvarlv pkelnflqke hrkavaedmi srtssdfnfm
      121 kriitgdetw vyecdmqpsq qsse
//
LOCUS       XP_034195536            1299 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611629 [Osmia lignaria lignaria].
ACCESSION   XP_034195536
VERSION     XP_034195536.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339645.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195536.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 3% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1299
                     /product="uncharacterized protein LOC117611629"
                     /calculated_mol_wt=149637
     Region          224..516
                     /region_name="Periplasmic_Binding_Protein_Type_2"
                     /note="Type 2 periplasmic binding fold superfamily;
                     cl21456"
                     /db_xref="CDD:473866"
     CDS             1..1299
                     /gene="LOC117611629"
                     /coded_by="XM_034339645.2:23..3922"
                     /db_xref="GeneID:117611629"
ORIGIN      
        1 mncvylllls tvqltavyca nenyiirdyf afrkvgrivg fscdnvendf klaktlngmy
       61 ttyismsnst iesrldvkkl ltaeyhqlgv fldcrcdpqr yikviadagk ysmydemhkw
      121 lilgsdlrqt leilnddtfs vstdvvivip sndnyllydv ynpckdrggs mnvtyfgsws
      181 sesrlnvsln qskfqrrsnl hgmklkvgvv lnikvenksl detmleysmk skygrskfly
      241 vllhhlsdlf nftmdlvqin aqrrfdnsgp vftafkkkli dmsaspvamk idrltngdii
      301 gpvwpirscf ifrtissskm kptqflqpls mkvwyvifat igmvtiilii lirmegirsp
      361 teiyglsvll tigaisqqgs afipnrfagr iallhtllfs vlilnyysas ivsnrlknkg
      421 ermndslisl aksnmklaae stpyirsflk vpdkevryfy enrwmkipef srylpleegl
      481 drvadgslay htmidsaypy ieqsfnyrsi celtevhlfr silafyarhk spftelmkig
      541 ltkirnvgiq krelkrwrsr kpfcpnnlli aeplsiheaa pvfiflsfsm flsllfclve
      601 nllfylssar resalmekkh ligrnfdlst lkdvnlqtsr nfnhqvyhag gfnptitkiv
      661 knllvtmsii fllllqfili snaqdhifir dyfvhkkvrn vvgfscgdti gdfnllktls
      721 tigiftiire psmkidfrrf lrsetwtvgv vidvrchnet avrifaessk hrmydysynw
      781 lvlgsnynrs ipllndtayn ivtdlvlais nkhgydlydv fnhckyrggl lnvtklgtwr
      841 qdsglnivlt qplinrranm hgmrlkisgv iqyrpkdmrl edymqdintr sldsmhkfvh
      901 ammlhtgdlf nfsvhaseii ywdrhsvhgl ifeflrsnyi dfasnprimv serldfasli
      961 gaawpikpcf mllststnki kleiflkpft rqtwyvfaaf gifsifimkl imnrekvgkr
     1021 ekysgavvls igiiaqqgan flpkrvpsri alfqitlhsw imynyysasi vsarlsepld
     1081 mmedsvtvla dsnlkiaaea vpylnyflyk lnwesdyfrk krwdplpesk rylpleegir
     1141 qvsqgilayh tdpntaypyv ermfdsnkic elteihlfkq svmgmyashn gqfieiakig
     1201 ltkmfntglr nrqikhwssr kpqcqpdtls trsitiyeta pallllafgi ivagticive
     1261 niifnrsmrk mdinqkkrig trgsiasgns rdnlldlnh
//
LOCUS       XP_034195538             517 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protease serine 9 [Osmia lignaria lignaria].
ACCESSION   XP_034195538
VERSION     XP_034195538.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339647.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195538.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..517
                     /product="transmembrane protease serine 9"
                     /calculated_mol_wt=56747
     Region          23..244
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; Many of these are
                     synthesized as inactive precursor zymogens that are
                     cleaved during limited proteolysis to generate their
                     active forms. Alignment contains also inactive enzymes
                     that have substitutions of the catalytic triad...;
                     cd00190"
                     /db_xref="CDD:238113"
     Site            23
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(63,108,202)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(196,217,219)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     Region          290..511
                     /region_name="Tryp_SPc"
                     /note="Trypsin-like serine protease; smart00020"
                     /db_xref="CDD:214473"
     Site            291
                     /site_type="active"
                     /note="cleavage site [active]"
                     /db_xref="CDD:238113"
     Site            order(331,376,472)
                     /site_type="active"
                     /db_xref="CDD:238113"
     Site            order(466,487,489)
                     /site_type="other"
                     /note="substrate binding sites [chemical binding]"
                     /db_xref="CDD:238113"
     CDS             1..517
                     /gene="LOC117611631"
                     /coded_by="XM_034339647.2:45..1598"
                     /db_xref="GeneID:117611631"
ORIGIN      
        1 mwrllvllsa swyvysyslg priiggentt idkypyqvsi qvggkhacgg siisenfvlt
       61 aahcvynelf qdvtvrvkst yseksgelig gvkfiwppqf dknildydia llklpnpikn
      121 vkpikladas tvvpdgskav vtgwgrlvpg ggmsstlqva tvptinwaec kkkyanlkpg
      181 vtermicass lngemnvcqg dsggplvfng vqigivswgd gctnrgnygv ytrvsslnnw
      241 iqntmkemyk flllsfrqlp amytclpfll lllapftlsq yflfqldegr ivggqpasid
      301 ehpyqvslrf nnrhicggai iseewvitaa hcvqtkfirf vsikagtsdl rddgivvfak
      361 qiishdnymr rnadydialv rlekplvyss wvrpillapi adhytagska vvtgwgvlrs
      421 dgpltdqlrk vevplvsnse cselyvtrpi tsrmicagyv nlggkdacqg dsggplvqyd
      481 kligivswgf gcarptypgv ytsitalrsw itektgl
//
LOCUS       XP_034195541             414 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611635 [Osmia lignaria lignaria].
ACCESSION   XP_034195541
VERSION     XP_034195541.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339650.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195541.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="uncharacterized protein LOC117611635"
                     /calculated_mol_wt=47250
     Region          233..373
                     /region_name="DUF4779"
                     /note="Domain of unknown function (DUF4779); pfam16009"
                     /db_xref="CDD:464971"
     CDS             1..414
                     /gene="LOC117611635"
                     /coded_by="XM_034339650.2:57..1301"
                     /db_xref="GeneID:117611635"
ORIGIN      
        1 mlsrtflicy islvvrndav ptriqrttmt ktrftdldgs atgytynqqq gvpvyyvqyt
       61 nqgsgryyht pdtvhyvles aapvasaipk ttlhqqpyat vhdvprtihq kheeaanygn
      121 qqslkhatsq prapyyvsek vthereekkd tydedveedh ddekkeldds eedddedvhg
      181 dsnggvikfn ysgrgsvdgs sfepadaege akkhsehgek hkeeehaehg ekgesgykks
      241 ekhskgeqgi ddeehqkgyy dikgghkkkh ldkaeehgsh eeaeeskksn dygqssshkk
      301 ghktsgyhnv yhkdeyknet dfydedhkeg hfdkheefdk gyktaeggsk kgghnsseqd
      361 hedrgkkgwy qkghrdnqnq ghqaeegeks ffddhehhds eedskfakth kseh
//
LOCUS       XP_076546699             446 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611638 [Osmia lignaria lignaria].
ACCESSION   XP_076546699
VERSION     XP_076546699.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690584.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 12% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..446
                     /product="uncharacterized protein LOC117611638"
                     /calculated_mol_wt=48752
     Region          294..342
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cl08302"
                     /db_xref="CDD:415501"
     Region          <320..430
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..446
                     /gene="LOC117611638"
                     /coded_by="XM_076690584.1:1..1341"
                     /db_xref="GeneID:117611638"
ORIGIN      
        1 mskvhacdis vffivslkii gcyvdsngld vsaetatrpp ppkpgrfvee shrivsnrnk
       61 fegnsvqctk easnisiqin tkgnnvvvre krarrslrlr reefagpsse rrgsaregar
      121 krtpgpvvvl sagggggggg ggsgttwgrg srgrgrdrgg ganligsgva panyssalqg
      181 vapelrevvn ilgernqvgt ltgvgpppea ldndsqgdee egttikrppr plyrrlinyi
      241 rqawtgvkfa ldseyeeeqt pryrpdslas lcratrftea elkriyrgfk aecptgvvre
      301 etfkciysqf fpqgantsqy ahyvfntldq dhsgilsfed fviglsilsr gsideklrwt
      361 fslydingdg citreemtdi vtavyelmgk fadpnldhqg vrekvdrmfq kmdgnkdgvv
      421 tlsefleacq adpdistsma aldtaf
//
LOCUS       XP_076546700            1209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305767 [Osmia lignaria lignaria].
ACCESSION   XP_076546700
VERSION     XP_076546700.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690585.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1209
                     /product="uncharacterized protein LOC143305767"
                     /calculated_mol_wt=137722
     Region          <3..103
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          230..443
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(294..299,332..333,368,370..371,434..435)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(294..299,332,370)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            333
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          459..619
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     Region          <727..788
                     /region_name="metallo-dependent_hydrolases"
                     /note="Superfamily of metallo-dependent hydrolases (also
                     called amidohydrolase superfamily) is a large group of
                     proteins that show conservation in their 3-dimensional
                     fold (TIM barrel) and in details of their active site. The
                     vast majority of the members have a...; cl00281"
                     /db_xref="CDD:469705"
     Region          830..885
                     /region_name="Integrase_H2C2"
                     /note="Integrase zinc binding domain; pfam17921"
                     /db_xref="CDD:465569"
     Region          1114..1205
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..1209
                     /gene="LOC143305767"
                     /coded_by="XM_076690585.1:1..3630"
                     /db_xref="GeneID:143305767"
ORIGIN      
        1 mntiasssin itfssihsdf kkdltclavp riadsvpeev fprelikipa nlrladpefh
       61 iprpvdilig sgatlsllsi gqiklsrdds dlflqktrlg wvivggagdn aekqavscal
      121 lelkeqiakf wlienidtet tglseealce thyrenttrd asgryivrlp frtgdrdfgd
      181 srrsafrrfn alqrklnana vlkaeytkvm qeyitlghms raddetvagy ymphhavikt
      241 tsattkvrvv fdasaksetn islndrlmig ptiqdklfeh lvrfrthayv ltadiekmyr
      301 qilvhpqdrk yqrifwyhed rvktyemntv tfgvssapyl airtiqqlad dertdfpyas
      361 eilkrdlyvd dlltgansle eilrirdeii ellkrggfni rqwasnhnha ldninekilg
      421 tdcvtddstv kktlglgwna redklvysvk sfelpakftk rvilseiaki fdplgllgpv
      481 ilaakvfmqe cwkaklewde svpqalcssw tafssqlhfv nnltvdrrli lknasniqih
      541 gfcdaskvgy gaciylrscd qdghtlvrls caksrvaplk emtmprlelc galtlarlyr
      601 etrsaidipi nrvtfwtdst ivlqwlrksp svlrlfesnr vaeiqktvea ewrhvrtkdn
      661 padalsrgqy paafcqnnsw fegpnwlrgr edswpinsvl tvtdlpglkk mtclaltink
      721 cdffinfssy pklvrvvayc lrmlrsnshr gelcaeekia aersiiraiq reqfsseiqh
      781 isssggakgn rlaslnpfid rdgllrvggr lqnaeisysq khpillpsyh hvtdliirdt
      841 hertyhagiq stlytirhrf wlldgknqvr kivrrcvrci rfratpvqrv mgnlpksrve
      901 eaaafthtgv dffgplfvke kkhrnrnrvk aygcvfvcms vkavhieivs dlttegflga
      961 frrfigrrai pthvysdngt nfvgannqlr elyalfgsdq fkgqvnefav sknitwhfnp
     1021 plsphfggiw eaavksfkhh fkrvvgerlf tfeeintfai eieailnsrp lcpissdpnd
     1081 plaltpahvl vgrpltilpe dnllsvpenr lsswrliika rqdfwkrwyt eylnelqkrq
     1141 kwleargeik vdtivllmdk nlpcmqwrlg rvleihpggd gevrvatikg angvfkrnis
     1201 vlcplledv
//
LOCUS       XP_076546701             427 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305768 [Osmia lignaria lignaria].
ACCESSION   XP_076546701
VERSION     XP_076546701.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690586.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="uncharacterized protein LOC143305768"
                     /calculated_mol_wt=48563
     Region          126..264
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     CDS             1..427
                     /gene="LOC143305768"
                     /coded_by="XM_076690586.1:1..1284"
                     /db_xref="GeneID:143305768"
ORIGIN      
        1 mdrirvinrr rttlktqvtq lvnnvasgrf nklvlksrla rvtdmltaye elndelaiid
       61 ptndrlddil eiqdqyyeca gaiegmedag tsgartnetl gntglnntli etrrtmklpv
      121 aelptfdgnf dewlsykntf ltmidartdi tdlerfiylk nslrgealkk viifsasven
      181 yksawqllad syekrrilis rhlnaildmk rlttatpsgl tkliddvrqh lsmlktldie
      241 pdhtmvicvl ekalpsdlrq kweelldedv fpslekfykf vsatanrlhh leevdsceks
      301 anqsgnkrrs drepraaktr kteasarsfv tatsiscfcc kqnhniykcp tfqkmttsqr
      361 wdtvkrkqlc rnclrphage ckgsrcrfcg rfhntllhsf anqqssagps tsqakpgiet
      421 tnqtkss
//
LOCUS       XP_034195511            1016 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611610 [Osmia lignaria lignaria].
ACCESSION   XP_034195511
VERSION     XP_034195511.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339620.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195511.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1016
                     /product="uncharacterized protein LOC117611610"
                     /calculated_mol_wt=118555
     CDS             1..1016
                     /gene="LOC117611610"
                     /coded_by="XM_034339620.2:401..3451"
                     /db_xref="GeneID:117611610"
ORIGIN      
        1 mskssmslsk istklskisf katddeattd ytttggesea tadeggnege kevteepgek
       61 rkkswiercv mrekwkcddk dlwrlymnty medreklekd difkrrkpap yrkfprvqse
      121 edlnahhkcl plnafqlrdq lalpnkldrk kmiriwrnmr anlpvhesks dtqidtqrdg
      181 vskttsdkwp gvwpcttrdl aneilerrrq krrtrtdlfp cdtdqiedef edpakdfsdr
      241 kelgharipq pcplpgkell vdraagehpg lvdgdyiife lpskakgari yadvclhglk
      301 gmhlpelgvl rarqktwkfc fyqaiialla ktqirykyaw svnidyiydh awmlythigm
      361 inikekqrld divvynykys ieveliqein dvpivtdipw dyeernmkrk mllerkelmn
      421 vqleigseki tdeqkaavhk gikkiedwfd klkirfrnci frttrfslaf wndnlfwyly
      481 npyrcdkfgy wdddgyacim kfcsrqslrr hlmilllray vysvpkpekq apesetvete
      541 eaataeeqpe attesasqgd aqsrttgdkq srtagdeqsr mtgdnqsrit gdnqsritgd
      601 nqsritgkeq srttteeqpq ttteeaasek eeaaeeeste ekfftvqiyh vvhrccqihn
      661 lkllqrgapk ppkhlvkkkt iddcpfdpfd mrdpctieqe egelkeaiek ptwlklykis
      721 wakclavpkk kgnkdfvvkm rwhqyvvees nklfslwgei hitdsmfeke nrgmqtyacy
      781 vvsagmtrim apeywtaktl dvivmcgdry ythskleaef kstkyeyshv ncwnrylmsh
      841 fkvgetmfea kvlpaicgrl yaksckylwh sleqmflkyh fgiltcestc lgvfkfcgay
      901 ymydvnsfgp plyqygegas yllrstyfyk fitvlvltig specsqfaln pieilkvvdv
      961 gpntlpvgra ekdrgrrvtk vecpydeekk rqmkkwrhkt aetvrtidrl cckstq
//
LOCUS       XP_034195545             770 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611639 [Osmia lignaria lignaria].
ACCESSION   XP_034195545
VERSION     XP_034195545.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339654.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195545.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..770
                     /product="uncharacterized protein LOC117611639"
                     /calculated_mol_wt=89340
     Region          69..370
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     Region          440..760
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..770
                     /gene="LOC117611639"
                     /coded_by="XM_034339654.2:166..2478"
                     /db_xref="GeneID:117611639"
ORIGIN      
        1 mndkkveikd vlllnervfs icdawplsns ykkfvmylsy lsvhlvimym dlydvmgele
       61 ylveniidnt iatttyfmlf lfrfskllkn vititkdels rnefrnneem qlylsyntis
      121 dnfgryavtt taviavmwyi tpmlellkat slsdkhnssk iyrlpfrvhs wvdyednlqn
      181 yilmyvyqip ltliallhis sislllslvl hvcgkfails yriqnitdds tdsfqrkvke
      241 mvdrhvelil ranslnsalq iflaieltqt sirmavlmyt illtddivni ftyglyvtiv
      301 tlmlylysyi gerleyesrk vndafydadw lklsirnqkf llycmsngrk tlhltagkyy
      361 afslsgfiqv givdsrvynn rrllqekarv kdvlvlneqi ysmcdvwpln ksytkfvmya
      421 sylfvhlivm ninlydvigd ltetvknitd saimtttfim ifqvrfskli rnviivvkke
      481 lasgdfenid eldlylsynk vsnnfgkyai rgsfitwlww ytgplihall sgsvtgkgnd
      541 tksfvlpfsa rafvdyednf qnymimfvyq lpimfvalfh tntvslifnl vmhvcgkfsi
      601 lshriqniqv dslsvnqfts kikelvnthl klivmtkmln saldiillie liqtsirmgv
      661 lvymlstemg nflntlrytl yiiavtaily lysyigecma qestrvieay ydfnwlklsi
      721 kdqktlllcm itgrrpmyit agkvyifslf gfigilktsf gfvsllqavi
//
LOCUS       XP_076546702            1452 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305769 [Osmia lignaria lignaria].
ACCESSION   XP_076546702
VERSION     XP_076546702.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690587.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 6% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1452
                     /product="uncharacterized protein LOC143305769"
                     /calculated_mol_wt=155690
     Region          1221..1242
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(1226,1228,1230,1232..1233,1236..1237,1241,1256,
                     1258..1260,1262..1263,1267)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          1249..1267
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..1452
                     /gene="LOC143305769"
                     /coded_by="XM_076690587.1:1..4359"
                     /db_xref="GeneID:143305769"
ORIGIN      
        1 mevriaekmv vggtastraq fvqyslarls sggdvvsspr gtgrdersvg grvkqatptt
       61 tvtttttatr tsasregggc dvgggvlggg rstggegtvs gggvgegggs vggggdvaek
      121 eerrpasesv eptaafdvas lvlygsralp irlkilldql fnqlqhaqvt rllaafgwty
      181 edytrgyilq nsqngpvldr wsicspeeep lvlqqflrfa etrpfvqqll laagtpgtda
      241 mspsrrpspp tmplahlpps lhllhcglpp pgsrlppplp pppppppppp psvshpsmsp
      301 taqkhyssmt ggnagvtsta nspprgldsh ltvsplnrlq smqpfdfrkl gtiglpafpp
      361 ippppsteql lqnrksmrnp hsphspphhs tsnqlqrphm ppvapvsssa lnfghplsva
      421 vssgalppsf pahfppppmg kssgavtgit aptdvhsgge kssefgseed eedeensdsa
      481 lnlsrrdskh anehrhhppl slqaaplgrp pgipgrkphs pgkrpgsqwg asanippnlg
      541 tpfinpttgk krvqcnvclk tfcdkgalki hfsavhlrem hkctvtgcsm mfssrrsrnr
      601 hsanpnpklh sphlrrkisp hdgrssmaha lvlppqvglp vpapglnhlp fgsfplltpp
      661 pdlrspaslc gldfkhldls saqnqarpgy eetlqqrmsv nasmsttalt tataamsnai
      721 etpnttmatr gsfsgssaad tvspstqast aaaegeeeee dedggivvva eddtsnlpsr
      781 lplrsgeedd dqpadfslak rpklyfghdt vdedlvsnpd snedndsmgt ldqqsysvsq
      841 hslnstslhs rgvrkrksqh ptrcavltei hssstdgdss ndedriqrrc lsesagissm
      901 ndfqnqpedm smsrleaegr kttpsspktl sfndqesnsr speacnrera aigtvhrdtv
      961 asqentedla eeqprdlssk spkrqispep ktshepaegd pvetttpatt nvqkesrscp
     1021 eqgdrvkckl eegtkeeeek sirrcakkeg eaeekakeee enqeeeeeee depmeeveed
     1081 dddeevndal rnqegerpdd psrapssvds rpttaddlsh dsstntlrql eslsqgramq
     1141 ytgmqqaasr sgrgstspvs ltthqettmd nssrgsrsss aspstaamdt dnslityary
     1201 enggfvstqe vpldrenprr ctacgkvfqn hfgvkthyqn vhlklmhrcs vdgcnaafps
     1261 krsrdrhsan lnlhrkllst ssspplsssl ppslsprved sqmnpllhne flarlyadag
     1321 isalgayplt pglpsavdla arippphpll lpphlgatfs glssfashla glnglthqhl
     1381 nhltrmsqeq gtrhvsgsgs pmsspgsddr keearctipg cdrvfgsrav rdahskdpqa
     1441 hrdlpvlsts gs
//
LOCUS       XP_034195656            2113 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4 gamma isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195656
VERSION     XP_034195656.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339765.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195656.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2113
                     /product="eukaryotic translation initiation factor 4 gamma
                     isoform X1"
                     /calculated_mol_wt=230935
     Region          <284..>494
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          439..>767
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          <800..>1071
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <1125..>1159
                     /region_name="eIF_4EBP"
                     /note="Eukaryotic translation initiation factor 4E binding
                     protein (EIF4EBP); pfam05456"
                     /db_xref="CDD:461656"
     Region          1255..1534
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          1744..1855
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          1942..2067
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..2113
                     /gene="Eif4g"
                     /coded_by="XM_034339765.2:755..7096"
                     /db_xref="GeneID:117611691"
ORIGIN      
        1 msmptkgkhh hlphhqhhsq phhnpsqhqp qllvnvnqps vhstqsyntv vtsntprfip
       61 nagvpvtlek eekvtsgrmv srygvskega vgvavgvgpm hcplphcggp hhhhhlsaph
      121 pqnpqpghnh hvhpahqpsp spsphlnhql shhqltvnin hlshqqqqqq qtqhqqappq
      181 yrvvtanars dfhvsgqnyg tqpggqvggg ggsvgvpggr gppplgglqs igqsgagipp
      241 ggpaggqapp qtsapgvqsq qpqgpapttt ppvhtpspqe mgkqahlqpq pqplsqvygp
      301 tqtrptsqgy ypgprpqqpr gishrggqgg tgaqvvgmsg vgggggqpta iyhpgglpvq
      361 tgamyqvpnl hpsphqqsvf amnsqiplqv vfggppqrhq thqnqsyfpp fqhsaiitaq
      421 nmfgygapap tpqpyfwptg qptntplsls rpgasavtgg aqhvagplas aqgapvvpqg
      481 tlqqptqqaq plppigisls qtdvysghng gasttnsttk srkprgqnai tdivdpstgk
      541 nisheiykdn etiqssessn retpqlqnnd aevqadfaaq vaktlakvat eestsdstvp
      601 tcvpitntnt sttqnvtqsl snsqtnsnnd vnsqcnttst aaqnipnvta lcsqslgttn
      661 nvtqidscvv kteskplqip vkefqprgei ktvviedppp pvprnvnkdd isaqllstta
      721 tevemkstsv psiatqtsvp pvttlpstnv peipkpstta pstnpvpare lfpnlsnkns
      781 ssspprrksq tythnqpata aelpssakeq kekktreksl ssrgttptpa hnqtdhhvks
      841 ngdatadkpe peaartevqq qkssdgkamq kqksknklkp relnrkgaek egtdmdafvn
      901 tvppakteak qdikeplpvk dsnkeetsre itkdikekdn yeskkekeis ssvhtktekq
      961 vtpstpeipk esttpaqapv leklsgnnet mkesspkied tnksnacnvq viksvpndvv
     1021 dhaivkeese veaivaqkne enskvsaari pteekpleas apvieksven etltqietts
     1081 svvattattt tttsaattpv snqitlkyky ndyqwspink tgkkvydref lmklqndpss
     1141 rikpsnlpdl dvvlkdnska rstvdlrpyk dtnmnrpdvl fpmfikvlpn arvpptnrks
     1201 hpgksskptk pnvihmslsl redvklrete nawrptrlkp islteeeakt ealykrvrsv
     1261 lnkltpqkfn tlvdqvraln idtperlqgv inlvfekavd epsfsvayal mckelammev
     1321 sggggtggga gggggggggg gggagagagp gaggtggggg gggggsekrd tyerqeysfs
     1381 fkkliinrcq nefeknpvne varaaklkei dectdperkk dlqvqleeee rririksvgn
     1441 irfigelykq gmltttimrr cikellnqnd edsleclckl lttigknler kgaademqdy
     1501 fnkmqdivsr rnqgkissri rfmlqdvidl rankwvprrd esnpktidqi qreaeserld
     1561 sqlsntplnt prkddrtndr krnrgigqtd esgwsqpirt rqtysvetak lknkpppmdd
     1621 lqlgsrnmym wktlsncgst ainsnkfacl dnvstldqdk rmpspqlsgs rstgpreygr
     1681 dykpsydgrg srngshqlss ssssressll dstqsqsvsm ppplkssqst tsnshkppmn
     1741 deefmkvlnk ifkdyfknln iekaasaiqq nfdstltkfi relinfvlek spidrehvsh
     1801 lmshlisqki lplqhlrngf ieileivdel vldipkvwsy laevlahpie ddilplselk
     1861 pifdslrsrg fvgrflgell skvsrdkgpk wvadkwdqsg lqlsnlidpe reevdkivnd
     1921 yniefctgdy nsaksstsnq ptlqqshnel rrlmkdsnfd vicdwiiani gnrvkepefi
     1981 rvlttaiiet smetannkwt lntdvfinlq qlirrfvdan eflelqclya iqlhlhrlqy
     2041 ppgilfdima slsennmiss dafltwkrcp epaqrewhgv atmlltsfft glqeaddass
     2101 vedvstsapq erc
//
LOCUS       XP_034195657            1985 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 4 gamma isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195657
VERSION     XP_034195657.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339766.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195657.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1985
                     /product="eukaryotic translation initiation factor 4 gamma
                     isoform X2"
                     /calculated_mol_wt=216890
     Region          311..>639
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     Region          <672..>943
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <997..>1031
                     /region_name="eIF_4EBP"
                     /note="Eukaryotic translation initiation factor 4E binding
                     protein (EIF4EBP); pfam05456"
                     /db_xref="CDD:461656"
     Region          1127..1406
                     /region_name="MIF4G"
                     /note="MIF4G domain; pfam02854"
                     /db_xref="CDD:397130"
     Region          1616..1727
                     /region_name="MA3"
                     /note="MA3 domain; cl02653"
                     /db_xref="CDD:413424"
     Region          1814..1939
                     /region_name="W2_eIF4G1_like"
                     /note="C-terminal W2 domain of eukaryotic translation
                     initiation factor 4 gamma 1 and similar proteins; cd11559"
                     /db_xref="CDD:211397"
     CDS             1..1985
                     /gene="Eif4g"
                     /coded_by="XM_034339766.2:748..6705"
                     /db_xref="GeneID:117611691"
ORIGIN      
        1 msmptkgkhh hlphhqhhsq phhnpsqhqp qllvnvnqps vhstqsyntv vtsntprfip
       61 nagsdfhvsg qnygtqpggq vgggggsvgv pggrgppplg glqsigqsga gippggpagg
      121 qappqtsapg vqsqqpqgpa ptttppvhtp spqemgkqah lqpqpqplsq vygptqtrpt
      181 sqgyypgprp qqprgishrg gqggtgaqvv gmsgvggggg qptaiyhpgg lpvqtgamyq
      241 vpnlhpsphq qsvfamnsqi plqfggppqr hqthqnqsyf ppfqhsaiit aqnmfgygap
      301 aptpqpyfwp tgqptntpls lsrpgasavt ggaqhvagpl asaqgapvvp qgtlqqptqq
      361 aqplppigis lsqtdvysgh nggasttnst tksrkprgqn aitdivdpst gknisheiyk
      421 dnetiqsses snretpqlqn ndaevqadfa aqvaktlakv ateestsdst vptcvpitnt
      481 ntsttqnvtq slsnsqtnsn ndvnsqcntt staaqnipnv talcsqslgt tnnvtqidsc
      541 vvkteskplq ipvkefqprg eiktvviedp pppvprnvnk ddisaqllst tatevemkst
      601 svpsiatqts vppvttlpst nvpeipkpst tapstnpvpa relfpnlsnk nsssspprrk
      661 sqtythnqpa taaelpssak eqkekktrek slssrgttpt pahnqtdhhv ksngdatadk
      721 pepeaartev qqqkssdgka mqkqksknkl kprelnrkga ekegtdmdaf vntvppakte
      781 akqdikeplp vkdsnkeets reitkdikek dnyeskkeke isssvhtkte kqvtpstpei
      841 pkesttpaqa pvleklsgnn etmkesspki edtnksnacn vqviksvpnd vvdhaivkee
      901 seveaivaqk neenskvsaa ripteekple asapvieksv enetltqiet tssvvattat
      961 tttttsaatt pvsnqitlky kyndyqwspi nktgkkvydr eflmklqndp ssrikpsnlp
     1021 dldvvlkdns karstvdlrp ykdtnmnrpd vlfpmfikvl pnarvpptnr kshpgksskp
     1081 tkpnvihmsl slredvklre tenawrptrl kpislteeea ktealykrvr svlnkltpqk
     1141 fntlvdqvra lnidtperlq gvinlvfeka vdepsfsvay almckelamm evsggggtgg
     1201 gagggggggg gggggagaga gpgaggtggg gggggggsek rdtyerqeys fsfkkliinr
     1261 cqnefeknpv nevaraaklk eidectdper kkdlqvqlee eerririksv gnirfigely
     1321 kqgmltttim rrcikellnq ndedsleclc kllttigknl erkgaademq dyfnkmqdiv
     1381 srrnqgkiss rirfmlqdvi dlrankwvpr rdesnpktid qiqreaeser ldsqlsntpl
     1441 ntprkddrtn drkrnrgigq tdesgwsqpi rtrqtysvet aklknkpppm ddlqlgsrnm
     1501 ymwktlsncg stainsnkfa cldnvstldq dkrmpspqls gsrstgprey grdykpsydg
     1561 rgsrngshql ssssssress lldstqsqsv smppplkssq sttsnshkpp mndeefmkvl
     1621 nkifkdyfkn lniekaasai qqnfdstltk firelinfvl ekspidrehv shlmshlisq
     1681 kilplqhlrn gfieileivd elvldipkvw sylaevlahp ieddilplse lkpifdslrs
     1741 rgfvgrflge llskvsrdkg pkwvadkwdq sglqlsnlid pereevdkiv ndyniefctg
     1801 dynsakssts nqptlqqshn elrrlmkdsn fdvicdwiia nignrvkepe firvlttaii
     1861 etsmetannk wtlntdvfin lqqlirrfvd aneflelqcl yaiqlhlhrl qyppgilfdi
     1921 maslsennmi ssdafltwkr cpepaqrewh gvatmlltsf ftglqeadda ssvedvstsa
     1981 pqerc
//
LOCUS       XP_034195658             815 aa            linear   INV 03-SEP-2025
DEFINITION  sarcolemma associated protein [Osmia lignaria lignaria].
ACCESSION   XP_034195658
VERSION     XP_034195658.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339767.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195658.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..815
                     /product="sarcolemma associated protein"
                     /calculated_mol_wt=92373
     Region          34..159
                     /region_name="FHA_SLMAP"
                     /note="forkhead associated (FHA) domain found in
                     sarcolemmal membrane-associated protein (SLMAP) and
                     similar proteins; cd22679"
                     /db_xref="CDD:438731"
     Site            order(62..64,81..84,86,103..106,136..138)
                     /site_type="other"
                     /note="putative phosphopeptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438731"
     Region          182..>501
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          182..244
                     /region_name="CC1_SLMAP-like"
                     /note="first coiled-coil (CC1) domain found in Sarcolemmal
                     membrane-associated protein and similar proteins; cl41740"
                     /db_xref="CDD:425371"
     Site            order(190,193..194,197,200..201,204,208,211,214..215,
                     218..219,221..222)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:409286"
     CDS             1..815
                     /gene="Slmap"
                     /coded_by="XM_034339767.2:1..2448"
                     /db_xref="GeneID:117611692"
ORIGIN      
        1 mvvasggwvq nasipanqnn snlnvntaqn nkmtakgili crdnshpfqd rmlsleqavk
       61 igrsvarara tpnnaifdck vlsrnhallw yaggkfflqd trssngtfvn nhrlspagle
      121 sapkevcsgd ivqfgvdvve ntkkvthgci vatlklylpn gieakaslst svtspvnnvs
      181 ledlyklnqv mqeasrreka lhykleylqq lvektrkaad qswkalited rllswvetie
      241 sqlavyseny kedairselv klqeekaqyq naakealhkv fleklevavt lyqtqaclsk
      301 tegecqslhn vakfaqselq evcskyteaq kklqettnkl eecenkmkdt vqnmeqekre
      361 lvkklehqaq ieknlqarlr dsrldsvniy kqitalknym qilqdmnskl vtssmmdskd
      421 ednpiniiln elnsihidna eldaetnlyp lkdeqdnqin sqdndsvqlk nsdlifskeq
      481 lddslsdndn lteyillpss rrtlvngngn gngnvninld nsdsnlesdt nsdatddtcs
      541 itsddtseks vievkkeeee eeedeeeaak areeiclpyi sevkfsseds dkqaafryse
      601 ivgklietev tversvesmn nvkilnepvs ktkellsege kkgetdkddn eldeefsceg
      661 ehlegeyvkt lkplskndss qsihtreyvl qtligsldsl kaeddfesqq vikkeleclk
      721 dwlicephed vigklkelyy rakneaqriq elheelvilk ekynilieek tdlskkyttl
      781 kaqcgdllnt tytvpihyva pivimliwml lekff
//
LOCUS       XP_034195659             433 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger CCCH domain-containing protein 10 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195659
VERSION     XP_034195659.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339768.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195659.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="zinc finger CCCH domain-containing protein 10
                     isoform X1"
                     /calculated_mol_wt=47667
     Region          40..58
                     /region_name="zf_CCCH_4"
                     /note="Zinc finger domain; pfam18345"
                     /db_xref="CDD:465719"
     Region          <247..288
                     /region_name="DUF6262"
                     /note="Family of unknown function (DUF6262); pfam19776"
                     /db_xref="CDD:466180"
     Region          <295..>417
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..433
                     /gene="LOC117611693"
                     /coded_by="XM_034339768.2:218..1519"
                     /db_xref="GeneID:117611693"
ORIGIN      
        1 mkklknklds vrggqpsktg aiavagapna ngdvsptrvc rdflrnvchr gkrckylher
       61 seddpveeyt fchdfqngmc nwpgckflhc tegeekrfra tgelpahimt rlknnnekse
      121 ypmckdfikg scqrtsckyr hwkndeqqhn litashhtvs rpqhnfngts ngenrryeed
      181 rnfhwqmddq hnlvtnngyn asshppdyig ppepkrrivs getvvhfeas plvgqhapqp
      241 vtpgyyypii prnearaivl edenvllrkk ieelkkqvsd ltatneflld qnaqlrmsgk
      301 rtanvtavtv pavtitntvp psqaptpqqm vnaavaagtl rtvtasvatv pvsiatvapv
      361 siaavsmapv sipppivtma qqtitmsgsg pqatnqqppn tqqpaslpls isgataplvs
      421 ypimtqelrp vlq
//
LOCUS       XP_034195660             433 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger CCCH domain-containing protein 10 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195660
VERSION     XP_034195660.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195660.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="zinc finger CCCH domain-containing protein 10
                     isoform X1"
                     /calculated_mol_wt=47667
     Region          40..58
                     /region_name="zf_CCCH_4"
                     /note="Zinc finger domain; pfam18345"
                     /db_xref="CDD:465719"
     Region          <247..288
                     /region_name="DUF6262"
                     /note="Family of unknown function (DUF6262); pfam19776"
                     /db_xref="CDD:466180"
     Region          <295..>417
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..433
                     /gene="LOC117611693"
                     /coded_by="XM_034339769.2:216..1517"
                     /db_xref="GeneID:117611693"
ORIGIN      
        1 mkklknklds vrggqpsktg aiavagapna ngdvsptrvc rdflrnvchr gkrckylher
       61 seddpveeyt fchdfqngmc nwpgckflhc tegeekrfra tgelpahimt rlknnnekse
      121 ypmckdfikg scqrtsckyr hwkndeqqhn litashhtvs rpqhnfngts ngenrryeed
      181 rnfhwqmddq hnlvtnngyn asshppdyig ppepkrrivs getvvhfeas plvgqhapqp
      241 vtpgyyypii prnearaivl edenvllrkk ieelkkqvsd ltatneflld qnaqlrmsgk
      301 rtanvtavtv pavtitntvp psqaptpqqm vnaavaagtl rtvtasvatv pvsiatvapv
      361 siaavsmapv sipppivtma qqtitmsgsg pqatnqqppn tqqpaslpls isgataplvs
      421 ypimtqelrp vlq
//
LOCUS       XP_034195662             433 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger CCCH domain-containing protein 10 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195662
VERSION     XP_034195662.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339771.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195662.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="zinc finger CCCH domain-containing protein 10
                     isoform X1"
                     /calculated_mol_wt=47667
     Region          40..58
                     /region_name="zf_CCCH_4"
                     /note="Zinc finger domain; pfam18345"
                     /db_xref="CDD:465719"
     Region          <247..288
                     /region_name="DUF6262"
                     /note="Family of unknown function (DUF6262); pfam19776"
                     /db_xref="CDD:466180"
     Region          <295..>417
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..433
                     /gene="LOC117611693"
                     /coded_by="XM_034339771.2:190..1491"
                     /db_xref="GeneID:117611693"
ORIGIN      
        1 mkklknklds vrggqpsktg aiavagapna ngdvsptrvc rdflrnvchr gkrckylher
       61 seddpveeyt fchdfqngmc nwpgckflhc tegeekrfra tgelpahimt rlknnnekse
      121 ypmckdfikg scqrtsckyr hwkndeqqhn litashhtvs rpqhnfngts ngenrryeed
      181 rnfhwqmddq hnlvtnngyn asshppdyig ppepkrrivs getvvhfeas plvgqhapqp
      241 vtpgyyypii prnearaivl edenvllrkk ieelkkqvsd ltatneflld qnaqlrmsgk
      301 rtanvtavtv pavtitntvp psqaptpqqm vnaavaagtl rtvtasvatv pvsiatvapv
      361 siaavsmapv sipppivtma qqtitmsgsg pqatnqqppn tqqpaslpls isgataplvs
      421 ypimtqelrp vlq
//
LOCUS       XP_034195663             409 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger CCCH domain-containing protein 10 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195663
VERSION     XP_034195663.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339772.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195663.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..409
                     /product="zinc finger CCCH domain-containing protein 10
                     isoform X2"
                     /calculated_mol_wt=44752
     Region          40..58
                     /region_name="zf_CCCH_4"
                     /note="Zinc finger domain; pfam18345"
                     /db_xref="CDD:465719"
     Region          <223..264
                     /region_name="DUF6262"
                     /note="Family of unknown function (DUF6262); pfam19776"
                     /db_xref="CDD:466180"
     Region          <271..>393
                     /region_name="PRK12323"
                     /note="DNA polymerase III subunit gamma/tau; cl46901"
                     /db_xref="CDD:481241"
     CDS             1..409
                     /gene="LOC117611693"
                     /coded_by="XM_034339772.2:218..1447"
                     /db_xref="GeneID:117611693"
ORIGIN      
        1 mkklknklds vrggqpsktg aiavagapna ngdvsptrvc rdflrnvchr gkrckylher
       61 seddpveeyt fchdfqngmc nwpgckflhc tegeekrfra tgelpahimt rlknnnekse
      121 ypmckdfika shhtvsrpqh nfngtsngen rryeedrnfh wqmddqhnlv tnngynassh
      181 ppdyigppep krrivsgetv vhfeasplvg qhapqpvtpg yyypiiprne araivleden
      241 vllrkkieel kkqvsdltat neflldqnaq lrmsgkrtan vtavtvpavt itntvppsqa
      301 ptpqqmvnaa vaagtlrtvt asvatvpvsi atvapvsiaa vsmapvsipp pivtmaqqti
      361 tmsgsgpqat nqqppntqqp aslplsisga taplvsypim tqelrpvlq
//
LOCUS       XP_034195664             247 aa            linear   INV 03-SEP-2025
DEFINITION  G patch domain-containing protein 11 [Osmia lignaria lignaria].
ACCESSION   XP_034195664
VERSION     XP_034195664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..247
                     /product="G patch domain-containing protein 11"
                     /calculated_mol_wt=28516
     Region          73..113
                     /region_name="G-patch"
                     /note="G-patch domain; pfam01585"
                     /db_xref="CDD:396249"
     Region          202..247
                     /region_name="DUF4187"
                     /note="Domain of unknown function (DUF4187); pfam13821"
                     /db_xref="CDD:463989"
     CDS             1..247
                     /gene="LOC117611694"
                     /coded_by="XM_034339773.2:339..1082"
                     /db_xref="GeneID:117611694"
ORIGIN      
        1 msdeedymsd kflqgsqkdt sstllyrhad krkfelmkkk seieaklkek gsakyieeek
       61 reaglssait stnkgfemlm kmgykpghgi gktesgmtep lgvevklnrh glgkeakkke
      121 vkvktnsisd kldnknmkdf rgriaqkrae qllkgdlyks qkiceqldmq lnlekpreiw
      181 fwlpqendes ddtdseedde tlpvnekldi ltkylrekhf yciwcgttfq ntedlrdncp
      241 gntrddh
//
LOCUS       XP_034195666             235 aa            linear   INV 03-SEP-2025
DEFINITION  dephospho-CoA kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195666
VERSION     XP_034195666.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339775.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..235
                     /product="dephospho-CoA kinase isoform X1"
                     /calculated_mol_wt=27014
     Region          1..194
                     /region_name="CoaE"
                     /note="Dephospho-CoA kinase [Coenzyme transport and
                     metabolism]; COG0237"
                     /db_xref="CDD:440007"
     CDS             1..235
                     /gene="Dpck"
                     /coded_by="XM_034339775.2:443..1150"
                     /db_xref="GeneID:117611695"
ORIGIN      
        1 mflvgltggi atgkssvaai freygipvid adqiarkvve aekpawhkik refgpevfld
       61 tgeldrvklg dlifndiekr kklnaithpd iyreiywqtf klflqghqfi vmdlpllfet
      121 ehmlnylyki ivvtceedlq lqrlmertgf teakaklrva aqmslekkae manfviensg
      181 serdtreqti kvinvlkask hhwklrfivg fcctvllagv ywirnknfrp lpita
//
LOCUS       XP_034195667             202 aa            linear   INV 03-SEP-2025
DEFINITION  dephospho-CoA kinase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195667
VERSION     XP_034195667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="dephospho-CoA kinase isoform X2"
                     /calculated_mol_wt=23714
     Region          6..161
                     /region_name="CoaE"
                     /note="Dephospho-CoA kinase [Coenzyme transport and
                     metabolism]; COG0237"
                     /db_xref="CDD:440007"
     CDS             1..202
                     /gene="Dpck"
                     /coded_by="XM_034339776.2:519..1127"
                     /db_xref="GeneID:117611695"
ORIGIN      
        1 mklkfveaek pawhkikref gpevfldtge ldrvklgdli fndiekrkkl naithpdiyr
       61 eiywqtfklf lqghqfivmd lpllfetehm lnylykiivv tceedlqlqr lmertgftea
      121 kaklrvaaqm slekkaeman fviensgser dtreqtikvi nvlkaskhhw klrfivgfcc
      181 tvllagvywi rnknfrplpi ta
//
LOCUS       XP_034195668             263 aa            linear   INV 03-SEP-2025
DEFINITION  bursicon [Osmia lignaria lignaria].
ACCESSION   XP_034195668
VERSION     XP_034195668.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339777.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195668.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="bursicon"
                     /calculated_mol_wt=29435
     Region          137..225
                     /region_name="DAN"
                     /note="DAN domain; pfam03045"
                     /db_xref="CDD:460786"
     CDS             1..263
                     /gene="LOC117611696"
                     /coded_by="XM_034339777.2:208..999"
                     /db_xref="GeneID:117611696"
ORIGIN      
        1 mfaifllmna liftsciiaq vtddencetl qsevhitkde ydeigrlkrt csgdvsvtkc
       61 egfcnsqvqp svasttgfsk ecyccresyl kerhitlrhc ydadgiklmn eedgimeiki
      121 repaecrcik caiigvdecq atpvihflqy pgcipkpips yacrgrcssy lqvsgskiwq
      181 merscmccqe sgereasvsl fcprakpgek kfrkvitkap lecmcrpcts veeyaiipqe
      241 iagfadegpf ttsahfrrss dlq
//
LOCUS       XP_034195534             563 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611627 [Osmia lignaria lignaria].
ACCESSION   XP_034195534
VERSION     XP_034195534.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339643.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..563
                     /product="uncharacterized protein LOC117611627"
                     /calculated_mol_wt=64046
     CDS             1..563
                     /gene="LOC117611627"
                     /coded_by="XM_034339643.2:135..1826"
                     /db_xref="GeneID:117611627"
ORIGIN      
        1 mntskwslvp itmlalistg spassnpveq qdlqkrhvpi anqslavqpy wehmlrefvf
       61 ktisppppgn nrlykrllda pfyagpfpmv sndvpsitls rgdvyylpng hwlfcqegci
      121 scevcsehth ptvkwvlrrv rrystqgssr hdlqltiipq tdgsyhmnnl wprsyvyvts
      181 qgeqaaywnd klgyhglgay vnledgssgp rektnnfwry vqrdsladrr hpgekkndti
      241 agetlekipk vdvesttaap leestttkka pskdlgkqrp rviqnnsdkk gtnvssisne
      301 nwknklkqlv pklilgtdqt gqkhlvhvvp advptnatsi nslasnllns tgqskiayqr
      361 ilrrifdsln snrrpienfl epfkqsrkis kpneehqqee tknglypvld ertrnesllh
      421 nrwpftlnwk rqrrksndgv fvsdftkstg npgwnsnhns nseiqnltds tinpyvnnrn
      481 gsvgfhpgii nrddysssas ndnlelmivh qnnrmyeftp rhfqlfvgpl ptrkpeeivr
      541 psknnqnqsd sipmhqsiel kdv
//
LOCUS       XP_034195549             406 aa            linear   INV 03-SEP-2025
DEFINITION  putative G-protein coupled receptor No18 [Osmia lignaria lignaria].
ACCESSION   XP_034195549
VERSION     XP_034195549.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339658.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..406
                     /product="putative G-protein coupled receptor No18"
                     /calculated_mol_wt=45082
     Region          39..371
                     /region_name="7tmA_tyramine_R-like"
                     /note="tyramine receptors and similar proteins, member of
                     the class A family of seven-transmembrane G
                     protein-coupled receptors; cd15061"
                     /db_xref="CDD:320189"
     Region          39..65
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320189"
     Region          72..98
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320189"
     Region          111..141
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320189"
     Site            order(114,118..119,122..123,192,199,202..203,206,320,
                     323..324,327,342,346,350)
                     /site_type="other"
                     /note="putative ligand binding site [chemical binding]"
                     /db_xref="CDD:320189"
     Region          154..177
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320189"
     Region          195..220
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320189"
     Region          300..330
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320189"
     Region          339..364
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320189"
     CDS             1..406
                     /gene="LOC117611642"
                     /coded_by="XM_034339658.2:634..1854"
                     /db_xref="GeneID:117611642"
ORIGIN      
        1 manqttnyyg dvyqwnstvs tterniqaey ylpnwtdlvl aglftmliiv tivgntlvia
       61 avittrrlrs vtncfvssla aadllvglav mppavllqlt ggtwelgavl cdswvsldil
      121 lctasilslc aisidrylav tqpliysrrr rskrlaglmi vavwvlagai tsppllgcfp
      181 ratnrdskkc synmdssyvi fsamgsfflp mlvmlyvygr iscviasrhr nleatetent
      241 rprrnvlier aksirirrte cgtgsvtcdr gtdeveppss sskksgivrs hqqscinrva
      301 retktagtla vvvggfvacw lpffilylat pfvpmappdi lmpaltwlgw insainpfiy
      361 afysadfrla fwrltcrkcf knrsnlersn rklptagtwk ketsrt
//
LOCUS       XP_034195768            1060 aa            linear   INV 03-SEP-2025
DEFINITION  kinesin-like protein 3A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195768
VERSION     XP_034195768.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339877.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1060
                     /product="kinesin-like protein 3A isoform X1"
                     /calculated_mol_wt=121905
     Region          7..332
                     /region_name="KISc_KIF4"
                     /note="Kinesin motor domain, KIF4-like subfamily; cd01372"
                     /db_xref="CDD:276823"
     Site            order(15,87,90,92..95,235)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276823"
     Site            order(284,287,290)
                     /site_type="other"
                     /note="microtubule interaction site [polypeptide binding]"
                     /db_xref="CDD:276823"
     Region          374..>918
                     /region_name="PRK03918"
                     /note="DNA double-strand break repair ATPase Rad50"
                     /db_xref="CDD:235175"
     Region          <594..>811
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          997..1029
                     /region_name="preSET_CXC"
                     /note="CXC domain; pfam18264"
                     /db_xref="CDD:408079"
     CDS             1..1060
                     /gene="Klp3A"
                     /coded_by="XM_034339877.2:187..3369"
                     /db_xref="GeneID:117611722"
ORIGIN      
        1 mtmsddtvrv avrirpliet eiergcqtcl evvpdepqiv irntdksftf nyvfppaigq
       61 edfyntavkd mvknifegyn vtilaygqtg sgkthsmgtn ysgadnsgvi pqavhdifdi
      121 vslkeewnfk itvsfmelyq eqlydlladk qrshsivdir ddgknikvtg vvenevtnaa
      181 etlqyltqgs lgratgatam nanssrshai ftlcvhqqkk snpdiattsk fhlvdlagse
      241 rskktqatge rfkegvnink gllalgnvis qlgegsttch vsyrdskltr llqdslggns
      301 mtlmiacvsp adynldetls tlryadrark iknkpvvnqd pkaaeinrln klvqelklal
      361 vgqeiktsip ieyeelelkn qslqekirnl reklntslie ivvmherael aeqarekiqs
      421 vmteilkeck ellddlnknp dkhsehcmkl qalyskifdi qndqqkisek lmnheisian
      481 aktisdekds eqtsidetny etpdtlddfd aknerytllq vkrnnevqni nkelaiketl
      541 isqllkdtsy iiddskerqe meqeikalqt ereellqalq nvhtskvssk laesrrkkvq
      601 elekkmaelr kkvveqdkiv kmkekqdqqi kkllsetqll kqtrvklirq mrtesdkftk
      661 wkqskekeln rlkdqnrkqt nevtrlkmwh nkqetvfkrk meeafaankr lkealdlqkr
      721 aamrkdkmsn ntdriknwlv qeieilestv aaehsleklm edrahltsil nelqnssdtd
      781 eqkiselvef lnvrniqiad lqqkivesdq enrayqrrqk iqtmedghta fkllckhite
      841 ekkrqcikes klleknallq aqldeyetrk knrrltpdns ndttesdnqe vaiiklreel
      901 eyyknqtqql krdlneikpg nkiskpkidk nvkktkesvd ldlptddsil edddpvndpd
      961 wtrtplynri qklmkttkns stehtalkrs sngdikcack tkcashlclc rknkiscnnc
     1021 scnpelcqnk ddninrtlfl dhkedetknt ikkprltgak
//
LOCUS       XP_034195769             964 aa            linear   INV 03-SEP-2025
DEFINITION  kinesin-like protein 3A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195769
VERSION     XP_034195769.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339878.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..964
                     /product="kinesin-like protein 3A isoform X2"
                     /calculated_mol_wt=111101
     Region          <1..236
                     /region_name="Motor_domain"
                     /note="Myosin and Kinesin motor domain; cl22853"
                     /db_xref="CDD:473979"
     Region          <275..544
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <498..>715
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          901..933
                     /region_name="preSET_CXC"
                     /note="CXC domain; pfam18264"
                     /db_xref="CDD:408079"
     CDS             1..964
                     /gene="Klp3A"
                     /coded_by="XM_034339878.2:222..3116"
                     /db_xref="GeneID:117611722"
ORIGIN      
        1 mgtnysgadn sgvipqavhd ifdivslkee wnfkitvsfm elyqeqlydl ladkqrshsi
       61 vdirddgkni kvtgvvenev tnaaetlqyl tqgslgratg atamnanssr shaiftlcvh
      121 qqkksnpdia ttskfhlvdl agserskktq atgerfkegv ninkgllalg nvisqlgegs
      181 ttchvsyrds kltrllqdsl ggnsmtlmia cvspadynld etlstlryad rarkiknkpv
      241 vnqdpkaaei nrlnklvqel klalvgqeik tsipieyeel elknqslqek irnlreklnt
      301 slieivvmhe raelaeqare kiqsvmteil keckellddl nknpdkhseh cmklqalysk
      361 ifdiqndqqk iseklmnhei sianaktisd ekdseqtsid etnyetpdtl ddfdaknery
      421 tllqvkrnne vqninkelai ketlisqllk dtsyiiddsk erqemeqeik alqtereell
      481 qalqnvhtsk vssklaesrr kkvqelekkm aelrkkvveq dkivkmkekq dqqikkllse
      541 tqllkqtrvk lirqmrtesd kftkwkqske kelnrlkdqn rkqtnevtrl kmwhnkqetv
      601 fkrkmeeafa ankrlkeald lqkraamrkd kmsnntdrik nwlvqeieil estvaaehsl
      661 eklmedrahl tsilnelqns sdtdeqkise lveflnvrni qiadlqqkiv esdqenrayq
      721 rrqkiqtmed ghtafkllck hiteekkrqc ikeskllekn allqaqldey etrkknrrlt
      781 pdnsndttes dnqevaiikl reeleyyknq tqqlkrdlne ikpgnkiskp kidknvkktk
      841 esvdldlptd dsiledddpv ndpdwtrtpl ynriqklmkt tknsstehta lkrssngdik
      901 cacktkcash lclcrknkis cnncscnpel cqnkddninr tlfldhkede tkntikkprl
      961 tgak
//
LOCUS       XP_034195770             871 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome biogenesis protein BOP1 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034195770
VERSION     XP_034195770.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339879.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195770.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..871
                     /product="ribosome biogenesis protein BOP1 homolog"
                     /calculated_mol_wt=99789
     Region          <17..234
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     Region          257..523
                     /region_name="BOP1NT"
                     /note="BOP1NT (NUC169) domain; pfam08145"
                     /db_xref="CDD:462371"
     Region          529..870
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(533,551,555,561..562,575,593,597,640..641,657..658,
                     675,680,689..690,703,720,730..731,743..744,762,766,771,
                     775,786..787,804,809,820..821,837..838,860,864,870)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          538..573
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          578..613
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          620..650
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          662..699
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          707..741
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          748..772
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          791..839
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          846..870
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..871
                     /gene="LOC117611723"
                     /coded_by="XM_034339879.2:32..2647"
                     /db_xref="GeneID:117611723"
ORIGIN      
        1 mkagkrvtkr klnslkvedn veekeknsmk seheeesdsg tedlllaevn nndestdeei
       61 denedvpesd tekdpivfes ddedelsfes dsddlgldes yseddsedss adqnnviknv
      121 gkknenlaak sqqlkskkev qksiseidnk nvsvasetkk rnknesnksn ipklaqsski
      181 qnkknlslnk nrettlkqni seksiktttk kgvalntsse nvdnvsvvnq pvdeyeydss
      241 deedvrntvg nipmkwydty dhigydwdgk kiikpqkgdq ldnflkrmed pdfwrtikdl
      301 qtgqdvvlse adielitriq kqkipeitfd eyapwiewft sevmktpvrk fpehkrsflp
      361 skpearkvsk lvhalkmgwm kstaeleker qqknsepqfy mlwqsddqse emrrihkhip
      421 ppkrhlpgha esynpppeyl fdkkelkewd klqatpwkrk lhfipqkyns lrevpaypky
      481 ikerfqrcld lylcpralkm rltiepealv pqlpspkdlq pfpttmsmvf kghadmvrsi
      541 taepmgqyva sggddmdlki weiatgrcvk tvpcggiirs vawcpnqsls liavaadkkv
      601 llinpgvgds litsktdqll qiipqsdviv sdrvktavqw eqaegehwsq girvilnhfk
      661 tvkqvtwhgk gdyfatvmpd gqnrsvlinq lskrrsqlpf nrskgliqcv lfhpirpylf
      721 vatqrnvriy dlvkqemikk llsnsqwiss maihpggdnv lvgtydrkml wfdldlstkp
      781 yqtlrlhgtg vrgvafhkry plfasgaddk gliishgmvy ndllqnpliv plkrlcnhes
      841 yndfgildvm fhpiqpwvfs agadatirmy t
//
LOCUS       XP_034195771             838 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195771
VERSION     XP_034195771.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339880.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="tubulin glycylase 3A isoform X1"
                     /calculated_mol_wt=97693
     Region          490..745
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..838
                     /gene="LOC117611724"
                     /coded_by="XM_034339880.2:16..2532"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 myfcspcirs rvpgcatklr itvedlpelc ktdiqpvner mqfdvsedeq wsratadnew
       61 nqqeqfehfn qvirelpdra mdlpidlrtf hlrrsrdvki akepipsdtt gnenkieqsn
      121 gkstalispe kkespmnpyn radwskmnak wsipdtedne insvnslsql dcgafpksym
      181 dtcsccprdi fdkhdtdtit sykgvtfkdg gccklhwtrk eryqniktkv graikrhkif
      241 lirgelpklk ealekrgwvq kyeatktrtl pygsvaslea rslgdltqpd gtlneksvvf
      301 allrhkapdf iwdcrndfvd whrglssnti lnryqkpsvy tsklgmarll eeahwlyekd
      361 vssvlfprsy nlsrepkafl ddfrltaavg llkwfvqtmq ddqtavqnhg qrlipisrle
      421 favkrceefi acenhqdide dfvsefseee wnsflddyaa avhesgriet tsergeeqlq
      481 kyhevanstl eklkevdpqy elngmrniwi lkpselccgt gisishnlrd ifrrvkskpk
      541 dyfivqkyie rpllihdtkf dirqwylvtn tfpmtiwlfk egllrfsskp ytfstyheai
      601 hicntaiqek ydeerrrrrk rgtseevvkt irdqgwdcdk lneylkqtgy egepyyekiy
      661 pkmseaivlt mlasqehmdr rrcsfelyga dfvvmedlsv wlieintnpr mhppssritk
      721 rlysnvlesl vkvvmdvpvn avadtggfsl vykqnipdfr pylgpclfvf gksitlqehp
      781 rkrekkkkgn csskqqqqqq qqqqhrawta ppmiprlrep kivdfidyln tarctaan
//
LOCUS       XP_076546703             838 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546703
VERSION     XP_076546703.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690588.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="tubulin glycylase 3A isoform X1"
                     /calculated_mol_wt=97693
     Region          490..745
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..838
                     /gene="LOC117611724"
                     /coded_by="XM_076690588.1:16..2532"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 myfcspcirs rvpgcatklr itvedlpelc ktdiqpvner mqfdvsedeq wsratadnew
       61 nqqeqfehfn qvirelpdra mdlpidlrtf hlrrsrdvki akepipsdtt gnenkieqsn
      121 gkstalispe kkespmnpyn radwskmnak wsipdtedne insvnslsql dcgafpksym
      181 dtcsccprdi fdkhdtdtit sykgvtfkdg gccklhwtrk eryqniktkv graikrhkif
      241 lirgelpklk ealekrgwvq kyeatktrtl pygsvaslea rslgdltqpd gtlneksvvf
      301 allrhkapdf iwdcrndfvd whrglssnti lnryqkpsvy tsklgmarll eeahwlyekd
      361 vssvlfprsy nlsrepkafl ddfrltaavg llkwfvqtmq ddqtavqnhg qrlipisrle
      421 favkrceefi acenhqdide dfvsefseee wnsflddyaa avhesgriet tsergeeqlq
      481 kyhevanstl eklkevdpqy elngmrniwi lkpselccgt gisishnlrd ifrrvkskpk
      541 dyfivqkyie rpllihdtkf dirqwylvtn tfpmtiwlfk egllrfsskp ytfstyheai
      601 hicntaiqek ydeerrrrrk rgtseevvkt irdqgwdcdk lneylkqtgy egepyyekiy
      661 pkmseaivlt mlasqehmdr rrcsfelyga dfvvmedlsv wlieintnpr mhppssritk
      721 rlysnvlesl vkvvmdvpvn avadtggfsl vykqnipdfr pylgpclfvf gksitlqehp
      781 rkrekkkkgn csskqqqqqq qqqqhrawta ppmiprlrep kivdfidyln tarctaan
//
LOCUS       XP_076546704             838 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546704
VERSION     XP_076546704.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690589.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="tubulin glycylase 3A isoform X1"
                     /calculated_mol_wt=97693
     Region          490..745
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..838
                     /gene="LOC117611724"
                     /coded_by="XM_076690589.1:16..2532"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 myfcspcirs rvpgcatklr itvedlpelc ktdiqpvner mqfdvsedeq wsratadnew
       61 nqqeqfehfn qvirelpdra mdlpidlrtf hlrrsrdvki akepipsdtt gnenkieqsn
      121 gkstalispe kkespmnpyn radwskmnak wsipdtedne insvnslsql dcgafpksym
      181 dtcsccprdi fdkhdtdtit sykgvtfkdg gccklhwtrk eryqniktkv graikrhkif
      241 lirgelpklk ealekrgwvq kyeatktrtl pygsvaslea rslgdltqpd gtlneksvvf
      301 allrhkapdf iwdcrndfvd whrglssnti lnryqkpsvy tsklgmarll eeahwlyekd
      361 vssvlfprsy nlsrepkafl ddfrltaavg llkwfvqtmq ddqtavqnhg qrlipisrle
      421 favkrceefi acenhqdide dfvsefseee wnsflddyaa avhesgriet tsergeeqlq
      481 kyhevanstl eklkevdpqy elngmrniwi lkpselccgt gisishnlrd ifrrvkskpk
      541 dyfivqkyie rpllihdtkf dirqwylvtn tfpmtiwlfk egllrfsskp ytfstyheai
      601 hicntaiqek ydeerrrrrk rgtseevvkt irdqgwdcdk lneylkqtgy egepyyekiy
      661 pkmseaivlt mlasqehmdr rrcsfelyga dfvvmedlsv wlieintnpr mhppssritk
      721 rlysnvlesl vkvvmdvpvn avadtggfsl vykqnipdfr pylgpclfvf gksitlqehp
      781 rkrekkkkgn csskqqqqqq qqqqhrawta ppmiprlrep kivdfidyln tarctaan
//
LOCUS       XP_076546705             838 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546705
VERSION     XP_076546705.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690590.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="tubulin glycylase 3A isoform X1"
                     /calculated_mol_wt=97693
     Region          490..745
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..838
                     /gene="LOC117611724"
                     /coded_by="XM_076690590.1:16..2532"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 myfcspcirs rvpgcatklr itvedlpelc ktdiqpvner mqfdvsedeq wsratadnew
       61 nqqeqfehfn qvirelpdra mdlpidlrtf hlrrsrdvki akepipsdtt gnenkieqsn
      121 gkstalispe kkespmnpyn radwskmnak wsipdtedne insvnslsql dcgafpksym
      181 dtcsccprdi fdkhdtdtit sykgvtfkdg gccklhwtrk eryqniktkv graikrhkif
      241 lirgelpklk ealekrgwvq kyeatktrtl pygsvaslea rslgdltqpd gtlneksvvf
      301 allrhkapdf iwdcrndfvd whrglssnti lnryqkpsvy tsklgmarll eeahwlyekd
      361 vssvlfprsy nlsrepkafl ddfrltaavg llkwfvqtmq ddqtavqnhg qrlipisrle
      421 favkrceefi acenhqdide dfvsefseee wnsflddyaa avhesgriet tsergeeqlq
      481 kyhevanstl eklkevdpqy elngmrniwi lkpselccgt gisishnlrd ifrrvkskpk
      541 dyfivqkyie rpllihdtkf dirqwylvtn tfpmtiwlfk egllrfsskp ytfstyheai
      601 hicntaiqek ydeerrrrrk rgtseevvkt irdqgwdcdk lneylkqtgy egepyyekiy
      661 pkmseaivlt mlasqehmdr rrcsfelyga dfvvmedlsv wlieintnpr mhppssritk
      721 rlysnvlesl vkvvmdvpvn avadtggfsl vykqnipdfr pylgpclfvf gksitlqehp
      781 rkrekkkkgn csskqqqqqq qqqqhrawta ppmiprlrep kivdfidyln tarctaan
//
LOCUS       XP_034195775             830 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195775
VERSION     XP_034195775.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339884.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..830
                     /product="tubulin glycylase 3A isoform X2"
                     /calculated_mol_wt=96639
     Region          482..737
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..830
                     /gene="LOC117611724"
                     /coded_by="XM_034339884.2:16..2508"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 myfcspcirs rvpgcatklr itvedlpelc ktdiqpvner mqfdvsedeq wsratadnew
       61 nqqeqfehfn qvirelpdra mdlpidlrdv kiakepipsd ttgnenkieq sngkstalis
      121 pekkespmnp ynradwskmn akwsipdted neinsvnsls qldcgafpks ymdtcsccpr
      181 difdkhdtdt itsykgvtfk dggccklhwt rkeryqnikt kvgraikrhk iflirgelpk
      241 lkealekrgw vqkyeatktr tlpygsvasl earslgdltq pdgtlneksv vfallrhkap
      301 dfiwdcrndf vdwhrglssn tilnryqkps vytsklgmar lleeahwlye kdvssvlfpr
      361 synlsrepka flddfrltaa vgllkwfvqt mqddqtavqn hgqrlipisr lefavkrcee
      421 fiacenhqdi dedfvsefse eewnsflddy aaavhesgri ettsergeeq lqkyhevans
      481 tleklkevdp qyelngmrni wilkpselcc gtgisishnl rdifrrvksk pkdyfivqky
      541 ierpllihdt kfdirqwylv tntfpmtiwl fkegllrfss kpytfstyhe aihicntaiq
      601 ekydeerrrr rkrgtseevv ktirdqgwdc dklneylkqt gyegepyyek iypkmseaiv
      661 ltmlasqehm drrrcsfely gadfvvmedl svwlieintn prmhppssri tkrlysnvle
      721 slvkvvmdvp vnavadtggf slvykqnipd frpylgpclf vfgksitlqe hprkrekkkk
      781 gncsskqqqq qqqqqqhraw tappmiprlr epkivdfidy lntarctaan
//
LOCUS       XP_034195776             798 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195776
VERSION     XP_034195776.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339885.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..798
                     /product="tubulin glycylase 3A isoform X3"
                     /calculated_mol_wt=93128
     Region          450..705
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..798
                     /gene="LOC117611724"
                     /coded_by="XM_034339885.2:165..2561"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 mqfdvsedeq wsratadnew nqqeqfehfn qvirelpdra mdlpidlrtf hlrrsrdvki
       61 akepipsdtt gnenkieqsn gkstalispe kkespmnpyn radwskmnak wsipdtedne
      121 insvnslsql dcgafpksym dtcsccprdi fdkhdtdtit sykgvtfkdg gccklhwtrk
      181 eryqniktkv graikrhkif lirgelpklk ealekrgwvq kyeatktrtl pygsvaslea
      241 rslgdltqpd gtlneksvvf allrhkapdf iwdcrndfvd whrglssnti lnryqkpsvy
      301 tsklgmarll eeahwlyekd vssvlfprsy nlsrepkafl ddfrltaavg llkwfvqtmq
      361 ddqtavqnhg qrlipisrle favkrceefi acenhqdide dfvsefseee wnsflddyaa
      421 avhesgriet tsergeeqlq kyhevanstl eklkevdpqy elngmrniwi lkpselccgt
      481 gisishnlrd ifrrvkskpk dyfivqkyie rpllihdtkf dirqwylvtn tfpmtiwlfk
      541 egllrfsskp ytfstyheai hicntaiqek ydeerrrrrk rgtseevvkt irdqgwdcdk
      601 lneylkqtgy egepyyekiy pkmseaivlt mlasqehmdr rrcsfelyga dfvvmedlsv
      661 wlieintnpr mhppssritk rlysnvlesl vkvvmdvpvn avadtggfsl vykqnipdfr
      721 pylgpclfvf gksitlqehp rkrekkkkgn csskqqqqqq qqqqhrawta ppmiprlrep
      781 kivdfidyln tarctaan
//
LOCUS       XP_034195777             758 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195777
VERSION     XP_034195777.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339886.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..758
                     /product="tubulin glycylase 3A isoform X4"
                     /calculated_mol_wt=88221
     Region          410..665
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..758
                     /gene="LOC117611724"
                     /coded_by="XM_034339886.2:446..2722"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 mdlpidlrtf hlrrsrdvki akepipsdtt gnenkieqsn gkstalispe kkespmnpyn
       61 radwskmnak wsipdtedne insvnslsql dcgafpksym dtcsccprdi fdkhdtdtit
      121 sykgvtfkdg gccklhwtrk eryqniktkv graikrhkif lirgelpklk ealekrgwvq
      181 kyeatktrtl pygsvaslea rslgdltqpd gtlneksvvf allrhkapdf iwdcrndfvd
      241 whrglssnti lnryqkpsvy tsklgmarll eeahwlyekd vssvlfprsy nlsrepkafl
      301 ddfrltaavg llkwfvqtmq ddqtavqnhg qrlipisrle favkrceefi acenhqdide
      361 dfvsefseee wnsflddyaa avhesgriet tsergeeqlq kyhevanstl eklkevdpqy
      421 elngmrniwi lkpselccgt gisishnlrd ifrrvkskpk dyfivqkyie rpllihdtkf
      481 dirqwylvtn tfpmtiwlfk egllrfsskp ytfstyheai hicntaiqek ydeerrrrrk
      541 rgtseevvkt irdqgwdcdk lneylkqtgy egepyyekiy pkmseaivlt mlasqehmdr
      601 rrcsfelyga dfvvmedlsv wlieintnpr mhppssritk rlysnvlesl vkvvmdvpvn
      661 avadtggfsl vykqnipdfr pylgpclfvf gksitlqehp rkrekkkkgn csskqqqqqq
      721 qqqqhrawta ppmiprlrep kivdfidyln tarctaan
//
LOCUS       XP_034195778             750 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3A isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034195778
VERSION     XP_034195778.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339887.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..750
                     /product="tubulin glycylase 3A isoform X5"
                     /calculated_mol_wt=87167
     Region          402..657
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..750
                     /gene="LOC117611724"
                     /coded_by="XM_034339887.2:445..2697"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 mdlpidlrdv kiakepipsd ttgnenkieq sngkstalis pekkespmnp ynradwskmn
       61 akwsipdted neinsvnsls qldcgafpks ymdtcsccpr difdkhdtdt itsykgvtfk
      121 dggccklhwt rkeryqnikt kvgraikrhk iflirgelpk lkealekrgw vqkyeatktr
      181 tlpygsvasl earslgdltq pdgtlneksv vfallrhkap dfiwdcrndf vdwhrglssn
      241 tilnryqkps vytsklgmar lleeahwlye kdvssvlfpr synlsrepka flddfrltaa
      301 vgllkwfvqt mqddqtavqn hgqrlipisr lefavkrcee fiacenhqdi dedfvsefse
      361 eewnsflddy aaavhesgri ettsergeeq lqkyhevans tleklkevdp qyelngmrni
      421 wilkpselcc gtgisishnl rdifrrvksk pkdyfivqky ierpllihdt kfdirqwylv
      481 tntfpmtiwl fkegllrfss kpytfstyhe aihicntaiq ekydeerrrr rkrgtseevv
      541 ktirdqgwdc dklneylkqt gyegepyyek iypkmseaiv ltmlasqehm drrrcsfely
      601 gadfvvmedl svwlieintn prmhppssri tkrlysnvle slvkvvmdvp vnavadtggf
      661 slvykqnipd frpylgpclf vfgksitlqe hprkrekkkk gncsskqqqq qqqqqqhraw
      721 tappmiprlr epkivdfidy lntarctaan
//
LOCUS       XP_034195779             701 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin glycylase 3B isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195779
VERSION     XP_034195779.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339888.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..701
                     /product="tubulin glycylase 3B isoform X6"
                     /calculated_mol_wt=81751
     Region          353..608
                     /region_name="CPSase_L_D2"
                     /note="Carbamoyl-phosphate synthase L chain, ATP binding
                     domain; cl17255"
                     /db_xref="CDD:473076"
     CDS             1..701
                     /gene="LOC117611724"
                     /coded_by="XM_034339888.2:17..2122"
                     /db_xref="GeneID:117611724"
ORIGIN      
        1 myfcspcirs rvpgcatklr itvedlpelc ktdiqpvner mqfdvsedeq wsratadnew
       61 nqqeqfehfn qvirelpdra mdlpidlrtf hlrrsrdvki akepipsdtt gnenkieqsn
      121 gkstalispe kkespmnpyn radwskmnak wsipdtedne insvnslsql dcgafpksym
      181 dtcsccprdi fdkhdtdtit sykgvtfkdg gccklhwtrk eryqniktkv graikrhkif
      241 lirgelpklk ealekrgwvq kyeatktrtl pygsvaslea rslgdltqpd gtlneksvvf
      301 allrhkapdf iwdcrndfvd whrglssnti lnryqkpsvy tskkyhevan stleklkevd
      361 pqyelngmrn iwilkpselc cgtgisishn lrdifrrvks kpkdyfivqk yierpllihd
      421 tkfdirqwyl vtntfpmtiw lfkegllrfs skpytfstyh eaihicntai qekydeerrr
      481 rrkrgtseev vktirdqgwd cdklneylkq tgyegepyye kiypkmseai vltmlasqeh
      541 mdrrrcsfel ygadfvvmed lsvwlieint nprmhppssr itkrlysnvl eslvkvvmdv
      601 pvnavadtgg fslvykqnip dfrpylgpcl fvfgksitlq ehprkrekkk kgncsskqqq
      661 qqqqqqqhra wtappmiprl repkivdfid ylntarctaa n
//
LOCUS       XP_034195780             627 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611725 [Osmia lignaria lignaria].
ACCESSION   XP_034195780
VERSION     XP_034195780.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339889.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195780.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..627
                     /product="uncharacterized protein LOC117611725"
                     /calculated_mol_wt=70590
     Site            order(86..87,127..129,131,138,142,166..171,175,177,179,
                     208..213)
                     /site_type="other"
                     /note="homodimer interaction site [polypeptide binding]"
                     /db_xref="CDD:187753"
     Region          99..218
                     /region_name="TrmO"
                     /note="tRNA-methyltransferase O; pfam01980"
                     /db_xref="CDD:460402"
     Site            order(141,143..144,171,179,181,200,205)
                     /site_type="active"
                     /note="cofactor binding site [active]"
                     /db_xref="CDD:187753"
     Region          527..587
                     /region_name="TrmO_C"
                     /note="TrmO C-terminal domain; pfam18389"
                     /db_xref="CDD:465738"
     CDS             1..627
                     /gene="LOC117611725"
                     /coded_by="XM_034339889.2:251..2134"
                     /db_xref="GeneID:117611725"
ORIGIN      
        1 mdcscqnsnf nakyllgqln tarkeinnlr qqikslryih ekdidtikhl lesfrnepsa
       61 srtkvigpdd vkpststdde aiklkpigvi stwfpskrgt prqtgicgkv pgklllynsv
      121 ftnpdhaleg lqdfshmwvl fyfhrndsth vrakvapprl ngtktgvfst rsphrpcpig
      181 lslvkitrie nhtiyfegvd mvdqspvldi kpyipqydsp lafdksnnrs qessindalg
      241 sieettrnqt cstfsaninv adetrnllsd syyrksssks sqetdisrde eialrlqaee
      301 fqrnsnfeny nnvrhffels dvnvhnnsil qhnidvtgih rlshtftntl sdtrsslnts
      361 vghntissnl eqqpeslidi nsrlqninvt ghtnveaayt srnnyvetha srsrlldgad
      421 gpntvcgtdi dliqrrslss ridnspvrmg vreapdgeeg fepqnlipsq slpnsqvirt
      481 tsdsslldsa ntnlvytsds gnaenreaet nsssevripd wisrprtplc vvfndraliq
      541 lneilgtkin dqkvaienvl redprsvylr qrwgsqfytf lihdlhitcr fddnrgivtv
      601 fqvrhagric ecgelewqcl ghsppps
//
LOCUS       XP_076546706             602 aa            linear   INV 03-SEP-2025
DEFINITION  KIF-binding protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546706
VERSION     XP_076546706.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690591.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="KIF-binding protein isoform X1"
                     /calculated_mol_wt=70327
     Region          232..595
                     /region_name="KBP_C"
                     /note="KIF-1 binding protein C terminal; pfam12309"
                     /db_xref="CDD:463528"
     CDS             1..602
                     /gene="LOC117611726"
                     /coded_by="XM_076690591.1:638..2446"
                     /db_xref="GeneID:117611726"
ORIGIN      
        1 meadttrqdm qadmrakfke vrkllddhek ftnerepyki kyqaievlmg metdltnalt
       61 nvpeeqlsie vimlavvhln lgilhtdtee lkageeqfmk cikllknkel epeailpvls
      121 alnqlgiiws qwnqpekaks fldqaeqiyn dfknmnrqcr dpvnmaqnfg ieevknelst
      181 kevlekihtl tlyylaqiys slkdhyksai ychmtlrrql ghkniaqdld yidwalntat
      241 lsqyfmennt fsqarhhlaa asyvlekyen ilkkklednk eseavaaewe tfkhrsadva
      301 rcwakygill mclskqrllq etetekkdqs dnkdssklki iddlkfdvle kdiepianqi
      361 tdkylltfdd arlvflnvqk wleqaksyyt lenhasdhiq iiqdisqayr ylaffeeqed
      421 rqakmhkrri dilenvikel npqyyksacr qiwielgeay seildikldr lrasnenpap
      481 qaltkinnla knaiknfenf fdsienrnsd sgmkpfpddi mqpaliayfh lgrlynkiit
      541 pdkaiqlent qnslnaykfv vdycekypka aelmhvelnl ckelvnllpm kisrlqetsr
      601 lq
//
LOCUS       XP_034195781             602 aa            linear   INV 03-SEP-2025
DEFINITION  KIF-binding protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195781
VERSION     XP_034195781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="KIF-binding protein isoform X1"
                     /calculated_mol_wt=70327
     Region          232..595
                     /region_name="KBP_C"
                     /note="KIF-1 binding protein C terminal; pfam12309"
                     /db_xref="CDD:463528"
     CDS             1..602
                     /gene="LOC117611726"
                     /coded_by="XM_034339890.2:736..2544"
                     /db_xref="GeneID:117611726"
ORIGIN      
        1 meadttrqdm qadmrakfke vrkllddhek ftnerepyki kyqaievlmg metdltnalt
       61 nvpeeqlsie vimlavvhln lgilhtdtee lkageeqfmk cikllknkel epeailpvls
      121 alnqlgiiws qwnqpekaks fldqaeqiyn dfknmnrqcr dpvnmaqnfg ieevknelst
      181 kevlekihtl tlyylaqiys slkdhyksai ychmtlrrql ghkniaqdld yidwalntat
      241 lsqyfmennt fsqarhhlaa asyvlekyen ilkkklednk eseavaaewe tfkhrsadva
      301 rcwakygill mclskqrllq etetekkdqs dnkdssklki iddlkfdvle kdiepianqi
      361 tdkylltfdd arlvflnvqk wleqaksyyt lenhasdhiq iiqdisqayr ylaffeeqed
      421 rqakmhkrri dilenvikel npqyyksacr qiwielgeay seildikldr lrasnenpap
      481 qaltkinnla knaiknfenf fdsienrnsd sgmkpfpddi mqpaliayfh lgrlynkiit
      541 pdkaiqlent qnslnaykfv vdycekypka aelmhvelnl ckelvnllpm kisrlqetsr
      601 lq
//
LOCUS       XP_034195782             602 aa            linear   INV 03-SEP-2025
DEFINITION  KIF-binding protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195782
VERSION     XP_034195782.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339891.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="KIF-binding protein isoform X1"
                     /calculated_mol_wt=70327
     Region          232..595
                     /region_name="KBP_C"
                     /note="KIF-1 binding protein C terminal; pfam12309"
                     /db_xref="CDD:463528"
     CDS             1..602
                     /gene="LOC117611726"
                     /coded_by="XM_034339891.2:673..2481"
                     /db_xref="GeneID:117611726"
ORIGIN      
        1 meadttrqdm qadmrakfke vrkllddhek ftnerepyki kyqaievlmg metdltnalt
       61 nvpeeqlsie vimlavvhln lgilhtdtee lkageeqfmk cikllknkel epeailpvls
      121 alnqlgiiws qwnqpekaks fldqaeqiyn dfknmnrqcr dpvnmaqnfg ieevknelst
      181 kevlekihtl tlyylaqiys slkdhyksai ychmtlrrql ghkniaqdld yidwalntat
      241 lsqyfmennt fsqarhhlaa asyvlekyen ilkkklednk eseavaaewe tfkhrsadva
      301 rcwakygill mclskqrllq etetekkdqs dnkdssklki iddlkfdvle kdiepianqi
      361 tdkylltfdd arlvflnvqk wleqaksyyt lenhasdhiq iiqdisqayr ylaffeeqed
      421 rqakmhkrri dilenvikel npqyyksacr qiwielgeay seildikldr lrasnenpap
      481 qaltkinnla knaiknfenf fdsienrnsd sgmkpfpddi mqpaliayfh lgrlynkiit
      541 pdkaiqlent qnslnaykfv vdycekypka aelmhvelnl ckelvnllpm kisrlqetsr
      601 lq
//
LOCUS       XP_034195783             602 aa            linear   INV 03-SEP-2025
DEFINITION  KIF-binding protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195783
VERSION     XP_034195783.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339892.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="KIF-binding protein isoform X1"
                     /calculated_mol_wt=70327
     Region          232..595
                     /region_name="KBP_C"
                     /note="KIF-1 binding protein C terminal; pfam12309"
                     /db_xref="CDD:463528"
     CDS             1..602
                     /gene="LOC117611726"
                     /coded_by="XM_034339892.2:519..2327"
                     /db_xref="GeneID:117611726"
ORIGIN      
        1 meadttrqdm qadmrakfke vrkllddhek ftnerepyki kyqaievlmg metdltnalt
       61 nvpeeqlsie vimlavvhln lgilhtdtee lkageeqfmk cikllknkel epeailpvls
      121 alnqlgiiws qwnqpekaks fldqaeqiyn dfknmnrqcr dpvnmaqnfg ieevknelst
      181 kevlekihtl tlyylaqiys slkdhyksai ychmtlrrql ghkniaqdld yidwalntat
      241 lsqyfmennt fsqarhhlaa asyvlekyen ilkkklednk eseavaaewe tfkhrsadva
      301 rcwakygill mclskqrllq etetekkdqs dnkdssklki iddlkfdvle kdiepianqi
      361 tdkylltfdd arlvflnvqk wleqaksyyt lenhasdhiq iiqdisqayr ylaffeeqed
      421 rqakmhkrri dilenvikel npqyyksacr qiwielgeay seildikldr lrasnenpap
      481 qaltkinnla knaiknfenf fdsienrnsd sgmkpfpddi mqpaliayfh lgrlynkiit
      541 pdkaiqlent qnslnaykfv vdycekypka aelmhvelnl ckelvnllpm kisrlqetsr
      601 lq
//
LOCUS       XP_034195784             602 aa            linear   INV 03-SEP-2025
DEFINITION  KIF-binding protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195784
VERSION     XP_034195784.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..602
                     /product="KIF-binding protein isoform X1"
                     /calculated_mol_wt=70327
     Region          232..595
                     /region_name="KBP_C"
                     /note="KIF-1 binding protein C terminal; pfam12309"
                     /db_xref="CDD:463528"
     CDS             1..602
                     /gene="LOC117611726"
                     /coded_by="XM_034339893.2:617..2425"
                     /db_xref="GeneID:117611726"
ORIGIN      
        1 meadttrqdm qadmrakfke vrkllddhek ftnerepyki kyqaievlmg metdltnalt
       61 nvpeeqlsie vimlavvhln lgilhtdtee lkageeqfmk cikllknkel epeailpvls
      121 alnqlgiiws qwnqpekaks fldqaeqiyn dfknmnrqcr dpvnmaqnfg ieevknelst
      181 kevlekihtl tlyylaqiys slkdhyksai ychmtlrrql ghkniaqdld yidwalntat
      241 lsqyfmennt fsqarhhlaa asyvlekyen ilkkklednk eseavaaewe tfkhrsadva
      301 rcwakygill mclskqrllq etetekkdqs dnkdssklki iddlkfdvle kdiepianqi
      361 tdkylltfdd arlvflnvqk wleqaksyyt lenhasdhiq iiqdisqayr ylaffeeqed
      421 rqakmhkrri dilenvikel npqyyksacr qiwielgeay seildikldr lrasnenpap
      481 qaltkinnla knaiknfenf fdsienrnsd sgmkpfpddi mqpaliayfh lgrlynkiit
      541 pdkaiqlent qnslnaykfv vdycekypka aelmhvelnl ckelvnllpm kisrlqetsr
      601 lq
//
LOCUS       XP_034195785             504 aa            linear   INV 03-SEP-2025
DEFINITION  KIF-binding protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195785
VERSION     XP_034195785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..504
                     /product="KIF-binding protein isoform X2"
                     /calculated_mol_wt=58964
     Region          134..497
                     /region_name="KBP_C"
                     /note="KIF-1 binding protein C terminal; pfam12309"
                     /db_xref="CDD:463528"
     CDS             1..504
                     /gene="LOC117611726"
                     /coded_by="XM_034339894.2:934..2448"
                     /db_xref="GeneID:117611726"
ORIGIN      
        1 mkcikllknk elepeailpv lsalnqlgii wsqwnqpeka ksfldqaeqi yndfknmnrq
       61 crdpvnmaqn fgieevknel stkevlekih tltlyylaqi ysslkdhyks aiychmtlrr
      121 qlghkniaqd ldyidwalnt atlsqyfmen ntfsqarhhl aaasyvleky enilkkkled
      181 nkeseavaae wetfkhrsad varcwakygi llmclskqrl lqetetekkd qsdnkdsskl
      241 kiiddlkfdv lekdiepian qitdkylltf ddarlvflnv qkwleqaksy ytlenhasdh
      301 iqiiqdisqa yrylaffeeq edrqakmhkr ridilenvik elnpqyyksa crqiwielge
      361 ayseildikl drlrasnenp apqaltkinn laknaiknfe nffdsienrn sdsgmkpfpd
      421 dimqpaliay fhlgrlynki itpdkaiqle ntqnslnayk fvvdycekyp kaaelmhvel
      481 nlckelvnll pmkisrlqet srlq
//
LOCUS       XP_034195786             543 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein S30 [Osmia lignaria lignaria].
ACCESSION   XP_034195786
VERSION     XP_034195786.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339895.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195786.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..543
                     /product="mitochondrial ribosomal protein S30"
                     /calculated_mol_wt=63886
     Region          30..422
                     /region_name="PDCD9"
                     /note="Mitochondrial 28S ribosomal protein S30 (PDCD9);
                     pfam07147"
                     /db_xref="CDD:462101"
     CDS             1..543
                     /gene="mRpS30"
                     /coded_by="XM_034339895.2:182..1813"
                     /db_xref="GeneID:117611728"
ORIGIN      
        1 myavlrksrt lknviqrcns ttstdtstva yppvldlayk arvrrkdeiw yekikkletv
       61 eeklyginmp ryygwkslcl kerhvlcnsl dqaqyitrth vvnnhelpdf ynnvitseql
      121 daviqiiknh ienilgfeyy nrlreqeite ktaenkkklq ddlingvvyq inrillatls
      181 snlphlmkaq vdyeprveaf wfiggidppt lvrkaransk hleayaddpv nmpvqyfgsp
      241 ilqlrhqfpl keivplneck npelnvpefk fdprtlghtf sykhatcipg fwpgdpdefg
      301 llsyhninns tnsevyyeds yivqaifasy gwllsqasyq gfstvydity pltsqtiftn
      361 gqewsfcvyq lnttlvhseh vdqnpkrnmc wvtkpmklfd tieegkvhgf nedvlrtlik
      421 fyvnmpeerl nvdmkpylgk tikyladied rdrrvflerg fkhissnrpk hylmpemyaw
      481 qkiyivdhkt rpidkkrdpw qfgykptkrr ldshlplyvp kclranpkkk rvdrwaktyy
      541 pda
//
LOCUS       XP_034195788             527 aa            linear   INV 03-SEP-2025
DEFINITION  apoptosis antagonizing transcription factor [Osmia lignaria
            lignaria].
ACCESSION   XP_034195788
VERSION     XP_034195788.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339897.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195788.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..527
                     /product="apoptosis antagonizing transcription factor"
                     /calculated_mol_wt=61779
     Region          186..360
                     /region_name="AATF-Che1"
                     /note="Apoptosis antagonizing transcription factor;
                     pfam13339"
                     /db_xref="CDD:463850"
     Region          432..515
                     /region_name="TRAUB"
                     /note="Apoptosis-antagonizing transcription factor,
                     C-terminal; pfam08164"
                     /db_xref="CDD:462388"
     CDS             1..527
                     /gene="Aatf"
                     /coded_by="XM_034339897.2:110..1693"
                     /db_xref="GeneID:117611729"
ORIGIN      
        1 malkakkksl adkintlitt tpinfgsdde aedtkakvve rydesddseg nfqvseirkr
       61 nvdpldqide rykgkkvsrk dvydneddsm eeeskneisd nedededdeg nddddddddd
      121 ddddnirvdd seyedkensn esnnsddmsy dsetdlkqhs edehksissk eekdfktmsh
      181 tnmredidkg ncvrsqlklw enmleirikl qrclntsnqm pqhdvytnfk sdteytkkad
      241 eaksqlkall nnmlqlqsfl lkqypetknl ltqnnkrkae enidkemkdl ddsmdeeips
      301 dtedenedkd tlvpaenvad fdehsfrkkl klnnyekiln dnhklytdyr nsviqkwnek
      361 tkiatgkvgk amnettlkqi efalsdkekt rkktrlkrse ykiigkaeat ddnegrrvqe
      421 ydseiydddd fyhqllrdli eykssditdp iqlskqwiql qnmrskmkrk idtkatkgrr
      481 irynvhnklv nfmapitvnd twtdhakdel ysslfgkikp inrevnr
//
LOCUS       XP_034195792             505 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal acid glucosylceramidase-like [Osmia lignaria lignaria].
ACCESSION   XP_034195792
VERSION     XP_034195792.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339901.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195792.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="lysosomal acid glucosylceramidase-like"
                     /calculated_mol_wt=56791
     Region          84..503
                     /region_name="XynC"
                     /note="O-Glycosyl hydrolase [Cell wall/membrane/envelope
                     biogenesis]; COG5520"
                     /db_xref="CDD:444271"
     CDS             1..505
                     /gene="LOC117611731"
                     /coded_by="XM_034339901.2:95..1612"
                     /db_xref="GeneID:117611731"
ORIGIN      
        1 mwwkvllltp fliaigstte cvprsfgtdk ivcvcnatyc dsieepkiqt tgsfysyvss
       61 kaglrmkpke gqfgschqtl ftdtltidtt kkyqtiygfg gaftdsagin ikklseaaqd
      121 qiirtyyhpt egskylfgrl giggtdfsir sysyddrpnd kslehfalap edykykipfm
      181 kkalelnpet kffaatwttp swmktknktn gygflkeeyy qsyadylvkf ldaykenglk
      241 mwaistgnep lfeylpsdai gilgwtpkgv gtwvannlgp tlaasqhdtk iltlddnrkd
      301 lswfvpqmfe nekakdyvsg vavhwysdhi tppsvldlah nsfpdkfilm teacegngsp
      361 qnvilgswer gqkymlsiie ylnnwaigwv dwnlaldkng gpnfvnnyvd spiivnpetd
      421 efykqpmyya iahfsrfidr gsvrisitdt ttvksaafvt psnevvvvlh ngnpesknvv
      481 lkdlkkgtic lelpansmht viygl
//
LOCUS       XP_034195789             505 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal acid glucosylceramidase-like [Osmia lignaria lignaria].
ACCESSION   XP_034195789
VERSION     XP_034195789.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339898.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195789.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="lysosomal acid glucosylceramidase-like"
                     /calculated_mol_wt=57184
     Region          84..503
                     /region_name="XynC"
                     /note="O-Glycosyl hydrolase [Cell wall/membrane/envelope
                     biogenesis]; COG5520"
                     /db_xref="CDD:444271"
     CDS             1..505
                     /gene="LOC117611730"
                     /coded_by="XM_034339898.2:388..1905"
                     /db_xref="GeneID:117611730"
ORIGIN      
        1 mwwkvllltp fliaigstte cvprsfgtdr ivcvcnatyc drieepkiqt nesfysyvss
       61 kaglrmkpke gqfgschqtl ftdtltidtt kkyqtifgfg gaftdsagin ikklsqaaqd
      121 qiirtyyhpt egsrylfgrl giggtdfsth aytyddhpnd ttlehfalap edynykipfm
      181 kkalelnpet rffgatwtpp iwmktndkyn gfgflkeeyy qlyanyilkf ldaykenglk
      241 mwaistgnep inaiipfdpl svmgwtpktv gnwvannlgp tlaaskhdtk ilalddqrie
      301 lpwfvqqmfe nekakdyvsg vavhwysdfi aspvlldlth nsfpekfilm teacegsgsp
      361 ekvilgswer gqkymlsile ylnnwavgwv dwnlaldksg gpnyinnnvd spiivnpetd
      421 efykqpmyya iahfsrfidr gsvrisitdt ttvksaafvt psnevvvvly nrnpvsknvv
      481 lkdlkkgtic lklpaysmht viygl
//
LOCUS       XP_034195790             505 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal acid glucosylceramidase-like [Osmia lignaria lignaria].
ACCESSION   XP_034195790
VERSION     XP_034195790.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339899.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195790.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="lysosomal acid glucosylceramidase-like"
                     /calculated_mol_wt=57184
     Region          84..503
                     /region_name="XynC"
                     /note="O-Glycosyl hydrolase [Cell wall/membrane/envelope
                     biogenesis]; COG5520"
                     /db_xref="CDD:444271"
     CDS             1..505
                     /gene="LOC117611730"
                     /coded_by="XM_034339899.2:69..1586"
                     /db_xref="GeneID:117611730"
ORIGIN      
        1 mwwkvllltp fliaigstte cvprsfgtdr ivcvcnatyc drieepkiqt nesfysyvss
       61 kaglrmkpke gqfgschqtl ftdtltidtt kkyqtifgfg gaftdsagin ikklsqaaqd
      121 qiirtyyhpt egsrylfgrl giggtdfsth aytyddhpnd ttlehfalap edynykipfm
      181 kkalelnpet rffgatwtpp iwmktndkyn gfgflkeeyy qlyanyilkf ldaykenglk
      241 mwaistgnep inaiipfdpl svmgwtpktv gnwvannlgp tlaaskhdtk ilalddqrie
      301 lpwfvqqmfe nekakdyvsg vavhwysdfi aspvlldlth nsfpekfilm teacegsgsp
      361 ekvilgswer gqkymlsile ylnnwavgwv dwnlaldksg gpnyinnnvd spiivnpetd
      421 efykqpmyya iahfsrfidr gsvrisitdt ttvksaafvt psnevvvvly nrnpvsknvv
      481 lkdlkkgtic lklpaysmht viygl
//
LOCUS       XP_076546707             505 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal acid glucosylceramidase-like [Osmia lignaria lignaria].
ACCESSION   XP_076546707
VERSION     XP_076546707.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690592.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="lysosomal acid glucosylceramidase-like"
                     /calculated_mol_wt=57184
     Region          84..503
                     /region_name="XynC"
                     /note="O-Glycosyl hydrolase [Cell wall/membrane/envelope
                     biogenesis]; COG5520"
                     /db_xref="CDD:444271"
     CDS             1..505
                     /gene="LOC117611730"
                     /coded_by="XM_076690592.1:378..1895"
                     /db_xref="GeneID:117611730"
ORIGIN      
        1 mwwkvllltp fliaigstte cvprsfgtdr ivcvcnatyc drieepkiqt nesfysyvss
       61 kaglrmkpke gqfgschqtl ftdtltidtt kkyqtifgfg gaftdsagin ikklsqaaqd
      121 qiirtyyhpt egsrylfgrl giggtdfsth aytyddhpnd ttlehfalap edynykipfm
      181 kkalelnpet rffgatwtpp iwmktndkyn gfgflkeeyy qlyanyilkf ldaykenglk
      241 mwaistgnep inaiipfdpl svmgwtpktv gnwvannlgp tlaaskhdtk ilalddqrie
      301 lpwfvqqmfe nekakdyvsg vavhwysdfi aspvlldlth nsfpekfilm teacegsgsp
      361 ekvilgswer gqkymlsile ylnnwavgwv dwnlaldksg gpnyinnnvd spiivnpetd
      421 efykqpmyya iahfsrfidr gsvrisitdt ttvksaafvt psnevvvvly nrnpvsknvv
      481 lkdlkkgtic lklpaysmht viygl
//
LOCUS       XP_034195793             403 aa            linear   INV 03-SEP-2025
DEFINITION  RAB6A-GEF complex partner protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195793
VERSION     XP_034195793.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339902.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="RAB6A-GEF complex partner protein 2 isoform X1"
                     /calculated_mol_wt=45334
     Region          <96..350
                     /region_name="Rgp1"
                     /note="pfam08737"
                     /db_xref="CDD:430181"
     CDS             1..403
                     /gene="LOC117611732"
                     /coded_by="XM_034339902.2:17..1228"
                     /db_xref="GeneID:117611732"
ORIGIN      
        1 mieitaklir gpvyfsgevi eclvtfsnpp npihqisqsn sdifeslawa saqvhcqcst
       61 nsklvvsekl nttarlvain anttfapwqq dnghvvlntk pkilfcdlrl spgesktyiy
      121 reiipsdapp syrghavkys ykitvgtqrv ntaikllrvp frvlslsgki dtfvlkkhvt
      181 yhltitiste lpevtacnds vdlspnnpfm etqhretsld valqtlqnlt arrspnfyni
      241 tngrgrvvrf clfknsyklg edivgtfdfs natvscaqvs valqseehis edyrrgknta
      301 ptlvsynkhh emcmglkysh lvlpiplhvt pdfttdlmtl kwrlhfefvt tsklietpnk
      361 ntmnwqgplt ldvetmiwdl pvhihptttp pntaqqtkyn ivi
//
LOCUS       XP_034195794             381 aa            linear   INV 03-SEP-2025
DEFINITION  RAB6A-GEF complex partner protein 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195794
VERSION     XP_034195794.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339903.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..381
                     /product="RAB6A-GEF complex partner protein 2 isoform X2"
                     /calculated_mol_wt=42836
     Region          <96..328
                     /region_name="Rgp1"
                     /note="pfam08737"
                     /db_xref="CDD:430181"
     CDS             1..381
                     /gene="LOC117611732"
                     /coded_by="XM_034339903.2:17..1162"
                     /db_xref="GeneID:117611732"
ORIGIN      
        1 mieitaklir gpvyfsgevi eclvtfsnpp npihqisqsn sdifeslawa saqvhcqcst
       61 nsklvvsekl nttarlvain anttfapwqq dnghvvlntk pkilfcdlrl spgesktyiy
      121 reiipsdapp syrghavkys ykitvgtqrv ntaikllrvp frvlslselp evtacndsvd
      181 lspnnpfmet qhretsldva lqtlqnltar rspnfynitn grgrvvrfcl fknsyklged
      241 ivgtfdfsna tvscaqvsva lqseehised yrrgkntapt lvsynkhhem cmglkyshlv
      301 lpiplhvtpd fttdlmtlkw rlhfefvtts klietpnknt mnwqgpltld vetmiwdlpv
      361 hihptttppn taqqtkyniv i
//
LOCUS       XP_034195795             380 aa            linear   INV 03-SEP-2025
DEFINITION  RAB6A-GEF complex partner protein 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195795
VERSION     XP_034195795.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339904.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..380
                     /product="RAB6A-GEF complex partner protein 2 isoform X3"
                     /calculated_mol_wt=42748
     Region          <96..327
                     /region_name="Rgp1"
                     /note="pfam08737"
                     /db_xref="CDD:430181"
     CDS             1..380
                     /gene="LOC117611732"
                     /coded_by="XM_034339904.2:17..1159"
                     /db_xref="GeneID:117611732"
ORIGIN      
        1 mieitaklir gpvyfsgevi eclvtfsnpp npihqisqsn sdifeslawa saqvhcqcst
       61 nsklvvsekl nttarlvain anttfapwqq dnghvvlntk pkilfcdlrl spgesktyiy
      121 reiipsdapp syrghavkys ykitvgtqrv ntaikllrvp frvlslklpe vtacndsvdl
      181 spnnpfmetq hretsldval qtlqnltarr spnfynitng rgrvvrfclf knsyklgedi
      241 vgtfdfsnat vscaqvsval qseehisedy rrgkntaptl vsynkhhemc mglkyshlvl
      301 piplhvtpdf ttdlmtlkwr lhfefvttsk lietpnkntm nwqgpltldv etmiwdlpvh
      361 ihptttppnt aqqtkynivi
//
LOCUS       XP_034195797             327 aa            linear   INV 03-SEP-2025
DEFINITION  RAB6A-GEF complex partner protein 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195797
VERSION     XP_034195797.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339906.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="RAB6A-GEF complex partner protein 2 isoform X4"
                     /calculated_mol_wt=37191
     Region          <20..274
                     /region_name="Rgp1"
                     /note="pfam08737"
                     /db_xref="CDD:430181"
     CDS             1..327
                     /gene="LOC117611732"
                     /coded_by="XM_034339906.2:117..1100"
                     /db_xref="GeneID:117611732"
ORIGIN      
        1 mqyftdttfa pwqqdnghvv lntkpkilfc dlrlspgesk tyiyreiips dappsyrgha
       61 vkysykitvg tqrvntaikl lrvpfrvlsl sgkidtfvlk khvtyhltit istelpevta
      121 cndsvdlspn npfmetqhre tsldvalqtl qnltarrspn fynitngrgr vvrfclfkns
      181 yklgedivgt fdfsnatvsc aqvsvalqse ehisedyrrg kntaptlvsy nkhhemcmgl
      241 kyshlvlpip lhvtpdfttd lmtlkwrlhf efvttsklie tpnkntmnwq gpltldvetm
      301 iwdlpvhihp tttppntaqq tkynivi
//
LOCUS       XP_034195798             340 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611733 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195798
VERSION     XP_034195798.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339907.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="uncharacterized protein LOC117611733 isoform X1"
                     /calculated_mol_wt=39217
     Region          40..>222
                     /region_name="XRCC4"
                     /note="DNA double-strand break repair and V(D)J
                     recombination protein XRCC4; pfam06632"
                     /db_xref="CDD:369011"
     CDS             1..340
                     /gene="LOC117611733"
                     /coded_by="XM_034339907.2:1..1023"
                     /db_xref="GeneID:117611733"
ORIGIN      
        1 mftikgvkqp ietysifitf knssimikin tceivnqvdn kcltlyvewl nshfkville
       61 ptmpplsgem vaedidyfck eldkssdeyl qetknifsgk deeiqfyiqd dilrwerkkt
      121 wtlgkitlcp vldikvfsea fqqllklyqs iqdkigtler enknlrdtna dllskinkmv
      181 eskatmeqdl ykkfililns kkkrirelea aikekqntae svfdahtdeg eesedddqkp
      241 ddnrikikkr ksldndnpes iqkarkknsi vnknhidnne tinktsvnkv ctmykahsin
      301 ystaskneni leepgcsstt kssrsslnfv eeesgdelfs
//
LOCUS       XP_034195799             293 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611733 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195799
VERSION     XP_034195799.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339908.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="uncharacterized protein LOC117611733 isoform X2"
                     /calculated_mol_wt=34204
     Region          40..>222
                     /region_name="XRCC4"
                     /note="DNA double-strand break repair and V(D)J
                     recombination protein XRCC4; pfam06632"
                     /db_xref="CDD:369011"
     CDS             1..293
                     /gene="LOC117611733"
                     /coded_by="XM_034339908.2:1..882"
                     /db_xref="GeneID:117611733"
ORIGIN      
        1 mftikgvkqp ietysifitf knssimikin tceivnqvdn kcltlyvewl nshfkville
       61 ptmpplsgem vaedidyfck eldkssdeyl qetknifsgk deeiqfyiqd dilrwerkkt
      121 wtlgkitlcp vldikvfsea fqqllklyqs iqdkigtler enknlrdtna dllskinkmv
      181 eskatmeqdl ykkfililns kkkrirelea aikekqntae svfdahtdeg eesedddqkp
      241 ddnrikikkr ksldndnpes iqkarkknsi hqkmrtflkn qdvalqpnhq evv
//
LOCUS       XP_034195801             275 aa            linear   INV 03-SEP-2025
DEFINITION  zwei Ig domain protein zig-8 [Osmia lignaria lignaria].
ACCESSION   XP_034195801
VERSION     XP_034195801.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339910.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="zwei Ig domain protein zig-8"
                     /calculated_mol_wt=30401
     Region          51..140
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          58..62
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          72..75
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          107..111
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          151..238
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          161..165
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          176..180
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          207..211
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          221..226
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          231..234
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..275
                     /gene="LOC117611735"
                     /coded_by="XM_034339910.2:1902..2729"
                     /db_xref="GeneID:117611735"
ORIGIN      
        1 mstslpvivc fiisfggias clrnfrtdfl eewptpgtgp hfdssmqsnf tglvgktvql
       61 vckvknlgnr tvswvrhrdi hlltvgryty tsdqrfealh yphteewtlk irypqrkdsg
      121 iyecqisttp pighpvylti vepitiivga pdlfvnkgst inltcivkya pepppmmiws
      181 hdtevinfds prggislvte kgpettsrlm iqkavpsdsg iytcapsnan pssikvhvvn
      241 eehpaamhhg dgsssyaktr pmcfiiliva nvifi
//
LOCUS       XP_034195802             149 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L14 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195802
VERSION     XP_034195802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339911.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="ribosomal protein L14 isoform X1"
                     /calculated_mol_wt=17372
     Region          5..122
                     /region_name="eL14"
                     /note="Vairimorpha necatrix ribosomal protein eL14 and
                     similar proteins; cd23702"
                     /db_xref="CDD:467915"
     CDS             1..149
                     /gene="RpL14"
                     /coded_by="XM_034339911.2:121..570"
                     /db_xref="GeneID:117611736"
ORIGIN      
        1 mpferfvesg rvayvsdgph lgklvtivdi idqnrvlvdg pstsvprcem rlnqlhltkf
       61 rlnfpftgst rvvrkawdaa kindlwketm warkveakkk rlelsdfdrf klrkarqirn
      121 klrtdafyrl kkkvkkvktt gankkvekk
//
LOCUS       XP_034195803             149 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L14 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195803
VERSION     XP_034195803.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339912.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="ribosomal protein L14 isoform X2"
                     /calculated_mol_wt=17372
     Region          5..122
                     /region_name="eL14"
                     /note="Vairimorpha necatrix ribosomal protein eL14 and
                     similar proteins; cd23702"
                     /db_xref="CDD:467915"
     CDS             1..149
                     /gene="RpL14"
                     /coded_by="XM_034339912.2:21..470"
                     /db_xref="GeneID:117611736"
ORIGIN      
        1 mpferfvesg rvayvsdgph lgklvtivdi idqnrvlvdg pstsvprcem rlnqlhltkf
       61 rlnfpftgst rvvrkawdaa kindlwketm warkveakkk rlelsdfdrf klrkarqirn
      121 klrtdafyrl kkkvkkvktt gankkvekk
//
LOCUS       XP_034195800             276 aa            linear   INV 03-SEP-2025
DEFINITION  salivary gland-expressed bHLH [Osmia lignaria lignaria].
ACCESSION   XP_034195800
VERSION     XP_034195800.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339909.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="salivary gland-expressed bHLH"
                     /calculated_mol_wt=32289
     Region          140..195
                     /region_name="bHLH_TS"
                     /note="tissue specific basic helix-loop-helix (bHLH-TS)
                     domain family; cd11390"
                     /db_xref="CDD:381396"
     Site            order(140,144,146..148,150,155,174..177)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381396"
     Site            order(153..154,157..158,160..161,164..165,177..178,
                     181..182,185,187..188,191..192,194..195)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381396"
     CDS             1..276
                     /gene="sage"
                     /coded_by="XM_034339909.2:16..846"
                     /db_xref="GeneID:117611734"
ORIGIN      
        1 mdseeytnel srnesendsn nedcthspil ldldknskqq qeilkqgens ttklppvgqy
       61 lqdqlikkrs enssvprrqs grkswqdgnf edrhqnsdrv fvirvpepev inthphleil
      121 hetniksvqr epsqtekdyk ksacdrertr mrdmnrafel lrsklpickp pgkklskies
      181 lrhaityirh lqsllepqys yvpnvpergy yptntsvtss tsfdvqhppr wesfayyryd
      241 yhtpfvnpts atlpppihpr vpteqddrqf pyesrh
//
LOCUS       XP_076546708             153 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305770 [Osmia lignaria lignaria].
ACCESSION   XP_076546708
VERSION     XP_076546708.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690593.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="uncharacterized protein LOC143305770"
                     /calculated_mol_wt=17358
     CDS             1..153
                     /gene="LOC143305770"
                     /coded_by="XM_076690593.1:2683..3144"
                     /db_xref="GeneID:143305770"
ORIGIN      
        1 mknemrqmcw etliplkevf ddvcrrypda actlsynsmk avlfrerqrn yphiptslta
       61 lnecivnhsf mrnicktvik denakvailf stetllqiln vsqtiyadgt fsvlpaqppi
      121 aqlyiihiqy mdtgvgtvfi lcealtkamy tai
//
LOCUS       XP_076546709             153 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305770 [Osmia lignaria lignaria].
ACCESSION   XP_076546709
VERSION     XP_076546709.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690594.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="uncharacterized protein LOC143305770"
                     /calculated_mol_wt=17358
     CDS             1..153
                     /gene="LOC143305770"
                     /coded_by="XM_076690594.1:2597..3058"
                     /db_xref="GeneID:143305770"
ORIGIN      
        1 mknemrqmcw etliplkevf ddvcrrypda actlsynsmk avlfrerqrn yphiptslta
       61 lnecivnhsf mrnicktvik denakvailf stetllqiln vsqtiyadgt fsvlpaqppi
      121 aqlyiihiqy mdtgvgtvfi lcealtkamy tai
//
LOCUS       XP_076546710             153 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305770 [Osmia lignaria lignaria].
ACCESSION   XP_076546710
VERSION     XP_076546710.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690595.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="uncharacterized protein LOC143305770"
                     /calculated_mol_wt=17358
     CDS             1..153
                     /gene="LOC143305770"
                     /coded_by="XM_076690595.1:2587..3048"
                     /db_xref="GeneID:143305770"
ORIGIN      
        1 mknemrqmcw etliplkevf ddvcrrypda actlsynsmk avlfrerqrn yphiptslta
       61 lnecivnhsf mrnicktvik denakvailf stetllqiln vsqtiyadgt fsvlpaqppi
      121 aqlyiihiqy mdtgvgtvfi lcealtkamy tai
//
LOCUS       XP_034196363             449 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611947 [Osmia lignaria lignaria].
ACCESSION   XP_034196363
VERSION     XP_034196363.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340472.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196363.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="uncharacterized protein LOC117611947"
                     /calculated_mol_wt=51915
     Region          346..379
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF domain; pfam07645"
                     /db_xref="CDD:429571"
     Site            order(346,349,367)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     CDS             1..449
                     /gene="LOC117611947"
                     /coded_by="XM_034340472.2:400..1749"
                     /db_xref="GeneID:117611947"
ORIGIN      
        1 mksilyaslc fcvlpelcsi qqmtfpiqel etttpisesr tatkeegeie avpmteqvid
       61 pdvrmtdvpd vdepsedwda ewdknkvird vayyirahkf qdydrryyrr pedsrgrlye
      121 efpkpplrsl hwevrkycda sfveclkyle gvikdtplkr eddtvtimke qkwkvenntk
      181 qiqavqrdcq taqrrdelta lpfqgpierf qwrstvsyym cwytmlgvpe lsifgescdn
      241 yancqmeheg gngdpraddt qpyacalysf cpdhccpirh irdmtdcyqs pvnpcyaanp
      301 pehrtctlnr qenqdffsli gnqiniscec mesgyewssr fglcvdvnec trgehscsin
      361 dgeacmnmpg gyqcickigy vydpgrrecv fsstihella geknetnvte tnnilemvvk
      421 aitrssdnsl rinysilflv mlmyflate
//
LOCUS       XP_034194772            2133 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis protein Ziz isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194772
VERSION     XP_034194772.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338881.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194772.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2133
                     /product="dedicator of cytokinesis protein Ziz isoform X1"
                     /calculated_mol_wt=243979
     Region          36..147
                     /region_name="DOCK_C-D_N"
                     /note="Dedicator of cytokinesis C/D, N terminal;
                     pfam11878"
                     /db_xref="CDD:463380"
     Region          159..286
                     /region_name="PH_DOCK-D"
                     /note="Dedicator of cytokinesis-D subfamily Pleckstrin
                     homology (PH) domain; cd13267"
                     /db_xref="CDD:270087"
     Region          655..845
                     /region_name="C2_Dock-D"
                     /note="C2 domains found in Dedicator Of CytoKinesis (Dock)
                     class C proteins; cd08697"
                     /db_xref="CDD:176079"
     Region          1675..2095
                     /region_name="DHR2_DOCK_D"
                     /note="Dock Homology Region 2, a GEF domain, of Class D
                     Dedicator of Cytokinesis proteins; cd11694"
                     /db_xref="CDD:212567"
     Site            order(1788,1798,1800..1801,1803..1804,1807..1808,
                     1810..1811,1814..1815)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            order(1822..1825,1856..1857,1859..1862,1864..1865,
                     1884..1886,1903,1905,1934,1936,1943..1944,1950,1952,
                     1989..1990,1993,2007,2009,2013,2016..2018,2021..2027,2030,
                     2058,2076..2077,2079..2080,2083)
                     /site_type="other"
                     /note="Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            2021..2026
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212567"
     CDS             1..2133
                     /gene="Ziz"
                     /coded_by="XM_034338881.2:367..6768"
                     /db_xref="GeneID:117610997"
ORIGIN      
        1 mserkftrgl gkpgmaaqlr etvsqvvres tvqnkphlve pidfesfvlk nktllqndpq
       61 relllypqdd vsqvvlprry rtlvptvsqt sdneegeenl ltkeclqsyt snwnlihyky
      121 saysgsylel pkiakaddlk eevyeidtev dqvdeeltks ngitkegylm kgpeigssdr
      181 mfanigsksf krrfchlrqe vdgtyilelf kdekkgeakl tivmdfctev vrnpkrgryc
      241 felrmsgthk sytlaadnet dmqdwllkls svlqhykqqe ekraaslera cntpppspqp
      301 mqvygtlkgl eqsmnpqlik ysretdtsia larrenrkrl fniypniphs kqqsgnssel
      361 nidpykeqfg qrifvkcesl kfrlqapide keslcqvepy qttlslydar hgrkltenfh
      421 fdinhevvqg makelspigi mtestedvkl pndlksipld wikypkqaif sisnphpdif
      481 lvvridkilq gnicqtsepy lratkdprlg lkvhkqvrac cqrlgnyrmp fawaarplfr
      541 lysneldvss dfpaiyrqeg nkikddellk llseyrkpek lskltvipgw lkikiesite
      601 lpdsmctwhi iiaytlstsl aalkpfplpp tseptleiae festsekdvh pyttyinhly
      661 vypqtlcfda qkiftrarni acivelrddd senaaplrci ygrpgspllc lrascavlhh
      721 navpswyeei kirlptklhs khhllfsfyh iscdmnkkke ngvescvgya wspllhkgrl
      781 nvdmdmnvqi lpvathlppg ylsiqplglg kgnagpeivw idsqrpvftv afqlistvft
      841 rdvhlhnlfa hmerildmki gavpadsetc kilkaahavq lvtiitflpt ilnqlfallt
      901 yttneevgly iirvlihfin mvhdagrket lqayikfvfv ppsqgngsit vheqlgkhlp
      961 tllqpnntdf lvvnkfmhhs nfffeimiks maqhllstgr ikmhrnerfs keyhekirnl
     1021 vevimpylmn kykempveth elnkslaqfl krcltfmdrg fvfrlinlyv dnfspgdqrt
     1081 lhdfkftflq iicshehyvs fnlpmmqsri tsrdlmneyc lsedfckhhf lvgllmqevk
     1141 tslneivqir kvaiatlrdl makhelddry qnkgqlsria fiyipwlgiv lenlhrlqsv
     1201 ydngkteikq ngtdristss sflankdtas nittvgtpks vhrltihldt qspirasmhl
     1261 rdstyfaaia gqglvngysc tsiesdtstm sgasqsnvsq ettiirepie ngtnekkrhs
     1321 rslsvtqssp rcdklqssev kdillcflfv ikylgdhqii awlqqcsdce mlsfftviem
     1381 slhhfkyvgk rqistnians sgkprtakam tlparmappd fstdapatst lqphntivre
     1441 nlvetdggkv hqaileanma tevglialdc lglfcihfkd vllatdgdnp imqkvfsiyl
     1501 sflqvgqset llrhvfasfr aflnnysiil fqgnavlcgr lcyellrccn sklscirqes
     1561 callyllmrs nfeftsrkgl trvhlqviis vsqmlgnvig lnnsrfqesl slinsyassd
     1621 kvmkgtgfpv evkdlnkrir tvlmataqmr ehnndpemlv dlqhslansy astpelrhtw
     1681 letmarnhar dgnfseaacc qlhiaalmae ylklrnvhsw gaeafdkise nisrdecslk
     1741 ldageicmfc tcvqdihyne yilleqlevc admlekaerf ellghlyrli vpmyeekrny
     1801 ealancyshl aqacnkivev tksgkrllgr fyrvaffgsa yfeeengqey iykepkitsl
     1861 seiserlhhl ysekfgaenv kmimdsvpid iteldpkisy iqvthvtayf ekfeletrqt
     1921 efeqnhniss fmfetpftke gkargspeeq wkrrtilktq ysfpyikkri lviqkrimel
     1981 spievaldem rqrvqeledv aligptdvkk lqlrlqgsic vtvnagplay asafldpals
     2041 pqypddkvee lkdvfrefvk icytalqins klitsdqhey qevlrenyqk lcqnlssllg
     2101 epiwpdeqvg nfkrnsaalf nvisgansht sta
//
LOCUS       XP_034194778            2128 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis protein Ziz isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194778
VERSION     XP_034194778.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338887.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194778.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2128
                     /product="dedicator of cytokinesis protein Ziz isoform X2"
                     /calculated_mol_wt=243393
     Region          36..147
                     /region_name="DOCK_C-D_N"
                     /note="Dedicator of cytokinesis C/D, N terminal;
                     pfam11878"
                     /db_xref="CDD:463380"
     Region          159..286
                     /region_name="PH_DOCK-D"
                     /note="Dedicator of cytokinesis-D subfamily Pleckstrin
                     homology (PH) domain; cd13267"
                     /db_xref="CDD:270087"
     Region          655..845
                     /region_name="C2_Dock-D"
                     /note="C2 domains found in Dedicator Of CytoKinesis (Dock)
                     class C proteins; cd08697"
                     /db_xref="CDD:176079"
     Region          1675..2090
                     /region_name="DHR2_DOCK_D"
                     /note="Dock Homology Region 2, a GEF domain, of Class D
                     Dedicator of Cytokinesis proteins; cd11694"
                     /db_xref="CDD:212567"
     Site            order(1783,1793,1795..1796,1798..1799,1802..1803,
                     1805..1806,1809..1810)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            order(1817..1820,1851..1852,1854..1857,1859..1860,
                     1879..1881,1898,1900,1929,1931,1938..1939,1945,1947,
                     1984..1985,1988,2002,2004,2008,2011..2013,2016..2022,2025,
                     2053,2071..2072,2074..2075,2078)
                     /site_type="other"
                     /note="Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            2016..2021
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212567"
     CDS             1..2128
                     /gene="Ziz"
                     /coded_by="XM_034338887.2:367..6753"
                     /db_xref="GeneID:117610997"
ORIGIN      
        1 mserkftrgl gkpgmaaqlr etvsqvvres tvqnkphlve pidfesfvlk nktllqndpq
       61 relllypqdd vsqvvlprry rtlvptvsqt sdneegeenl ltkeclqsyt snwnlihyky
      121 saysgsylel pkiakaddlk eevyeidtev dqvdeeltks ngitkegylm kgpeigssdr
      181 mfanigsksf krrfchlrqe vdgtyilelf kdekkgeakl tivmdfctev vrnpkrgryc
      241 felrmsgthk sytlaadnet dmqdwllkls svlqhykqqe ekraaslera cntpppspqp
      301 mqvygtlkgl eqsmnpqlik ysretdtsia larrenrkrl fniypniphs kqqsgnssel
      361 nidpykeqfg qrifvkcesl kfrlqapide keslcqvepy qttlslydar hgrkltenfh
      421 fdinhevvqg makelspigi mtestedvkl pndlksipld wikypkqaif sisnphpdif
      481 lvvridkilq gnicqtsepy lratkdprlg lkvhkqvrac cqrlgnyrmp fawaarplfr
      541 lysneldvss dfpaiyrqeg nkikddellk llseyrkpek lskltvipgw lkikiesite
      601 lpdsmctwhi iiaytlstsl aalkpfplpp tseptleiae festsekdvh pyttyinhly
      661 vypqtlcfda qkiftrarni acivelrddd senaaplrci ygrpgspllc lrascavlhh
      721 navpswyeei kirlptklhs khhllfsfyh iscdmnkkke ngvescvgya wspllhkgrl
      781 nvdmdmnvqi lpvathlppg ylsiqplglg kgnagpeivw idsqrpvftv afqlistvft
      841 rdvhlhnlfa hmerildmki gavpadsetc kilkaahavq lvtiitflpt ilnqlfallt
      901 yttneevgly iirvlihfin mvhdagrket lqayikfvfv ppsqgngsit vheqlgkhlp
      961 tllqpnntdf lvvnkfmhhs nfffeimiks maqhllstgr ikmhrnerfs keyhekirnl
     1021 vevimpylmn kykempveth elnkslaqfl krcltfmdrg fvfrlinlyv dnfspgdqrt
     1081 lhdfkftflq iicshehyvs fnlpmmqsri tsrdlmneyc lsedfckhhf lvgllmqevk
     1141 tslneivqir kvaiatlrdl makhelddry qnkgqlsria fiyipwlgiv lenlhrlqsv
     1201 ydngkteikq ngtdristss sflankdtas nittvgtpks vhrltihldt qspirasmhl
     1261 rdstyfaaia gqglvngysc tsiesdtstm sgasqsnvsq ettiirepie ngtnekkrhs
     1321 rslsvtqssp rcdklqssev kdillcflfv ikylgdhqii awlqqcsdce mlsfftviem
     1381 slhhfkyvgk rqistnians sgkprtakam tlparmappd fstdapatst lqphntivre
     1441 nlvetdggkv hqaileanma tevglialdc lglfcihfkd vllatdgdnp imqkvfsiyl
     1501 sflqvgqset llrhvfasfr aflnnysiil fqgnavlcgr lcyellrccn sklscirqes
     1561 callyllmrs nfeftsrkgl trvhlqviis vsqmlgnvig lnnsrfqesl slinsyassd
     1621 kvmkgtgfpv evkdlnkrir tvlmataqmr ehnndpemlv dlqhslansy astpelrhtw
     1681 letmarnhar dgnfseaacc qlhiaalmae ylklrnvhsw gaeafdkise nisrdecslk
     1741 ldageicvqd ihyneyille qlevcadmle kaerfellgh lyrlivpmye ekrnyealan
     1801 cyshlaqacn kivevtksgk rllgrfyrva ffgsayfeee ngqeyiykep kitslseise
     1861 rlhhlysekf gaenvkmimd svpiditeld pkisyiqvth vtayfekfel etrqtefeqn
     1921 hnissfmfet pftkegkarg speeqwkrrt ilktqysfpy ikkrilviqk rimelspiev
     1981 aldemrqrvq eledvaligp tdvkklqlrl qgsicvtvna gplayasafl dpalspqypd
     2041 dkveelkdvf refvkicyta lqinsklits dqheyqevlr enyqklcqnl ssllgepiwp
     2101 deqvgnfkrn saalfnvisg anshtsta
//
LOCUS       XP_034194786            2125 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis protein Ziz isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194786
VERSION     XP_034194786.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338895.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194786.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2125
                     /product="dedicator of cytokinesis protein Ziz isoform X3"
                     /calculated_mol_wt=243047
     Region          36..147
                     /region_name="DOCK_C-D_N"
                     /note="Dedicator of cytokinesis C/D, N terminal;
                     pfam11878"
                     /db_xref="CDD:463380"
     Region          159..286
                     /region_name="PH_DOCK-D"
                     /note="Dedicator of cytokinesis-D subfamily Pleckstrin
                     homology (PH) domain; cd13267"
                     /db_xref="CDD:270087"
     Region          655..845
                     /region_name="C2_Dock-D"
                     /note="C2 domains found in Dedicator Of CytoKinesis (Dock)
                     class C proteins; cd08697"
                     /db_xref="CDD:176079"
     Region          1675..2087
                     /region_name="DHR2_DOCK_D"
                     /note="Dock Homology Region 2, a GEF domain, of Class D
                     Dedicator of Cytokinesis proteins; cd11694"
                     /db_xref="CDD:212567"
     Site            order(1780,1790,1792..1793,1795..1796,1799..1800,
                     1802..1803,1806..1807)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            order(1814..1817,1848..1849,1851..1854,1856..1857,
                     1876..1878,1895,1897,1926,1928,1935..1936,1942,1944,
                     1981..1982,1985,1999,2001,2005,2008..2010,2013..2019,2022,
                     2050,2068..2069,2071..2072,2075)
                     /site_type="other"
                     /note="Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            2013..2018
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212567"
     CDS             1..2125
                     /gene="Ziz"
                     /coded_by="XM_034338895.2:367..6744"
                     /db_xref="GeneID:117610997"
ORIGIN      
        1 mserkftrgl gkpgmaaqlr etvsqvvres tvqnkphlve pidfesfvlk nktllqndpq
       61 relllypqdd vsqvvlprry rtlvptvsqt sdneegeenl ltkeclqsyt snwnlihyky
      121 saysgsylel pkiakaddlk eevyeidtev dqvdeeltks ngitkegylm kgpeigssdr
      181 mfanigsksf krrfchlrqe vdgtyilelf kdekkgeakl tivmdfctev vrnpkrgryc
      241 felrmsgthk sytlaadnet dmqdwllkls svlqhykqqe ekraaslera cntpppspqp
      301 mqvygtlkgl eqsmnpqlik ysretdtsia larrenrkrl fniypniphs kqqsgnssel
      361 nidpykeqfg qrifvkcesl kfrlqapide keslcqvepy qttlslydar hgrkltenfh
      421 fdinhevvqg makelspigi mtestedvkl pndlksipld wikypkqaif sisnphpdif
      481 lvvridkilq gnicqtsepy lratkdprlg lkvhkqvrac cqrlgnyrmp fawaarplfr
      541 lysneldvss dfpaiyrqeg nkikddellk llseyrkpek lskltvipgw lkikiesite
      601 lpdsmctwhi iiaytlstsl aalkpfplpp tseptleiae festsekdvh pyttyinhly
      661 vypqtlcfda qkiftrarni acivelrddd senaaplrci ygrpgspllc lrascavlhh
      721 navpswyeei kirlptklhs khhllfsfyh iscdmnkkke ngvescvgya wspllhkgrl
      781 nvdmdmnvqi lpvathlppg ylsiqplglg kgnagpeivw idsqrpvftv afqlistvft
      841 rdvhlhnlfa hmerildmki gavpadsetc kilkaahavq lvtiitflpt ilnqlfallt
      901 yttneevgly iirvlihfin mvhdagrket lqayikfvfv ppsqgngsit vheqlgkhlp
      961 tllqpnntdf lvvnkfmhhs nfffeimiks maqhllstgr ikmhrnerfs keyhekirnl
     1021 vevimpylmn kykempveth elnkslaqfl krcltfmdrg fvfrlinlyv dnfspgdqrt
     1081 lhdfkftflq iicshehyvs fnlpmmqsri tsrdlmneyc lsedfckhhf lvgllmqevk
     1141 tslneivqir kvaiatlrdl makhelddry qnkgqlsria fiyipwlgiv lenlhrlqsv
     1201 ydngkteikq ngtdristss sflankdtas nittvgtpks vhrltihldt qspirasmhl
     1261 rdstyfaaia gqglvngysc tsiesdtstm sgasqsnvsq ettiirepie ngtnekkrhs
     1321 rslsvtqssp rcdklqssev kdillcflfv ikylgdhqii awlqqcsdce mlsfftviem
     1381 slhhfkyvgk rqistnians sgkprtakam tlparmappd fstdapatst lqphntivre
     1441 nlvetdggkv hqaileanma tevglialdc lglfcihfkd vllatdgdnp imqkvfsiyl
     1501 sflqvgqset llrhvfasfr aflnnysiil fqgnavlcgr lcyellrccn sklscirqes
     1561 callyllmrs nfeftsrkgl trvhlqviis vsqmlgnvig lnnsrfqesl slinsyassd
     1621 kvmkgtgfpv evkdlnkrir tvlmataqmr ehnndpemlv dlqhslansy astpelrhtw
     1681 letmarnhar dgnfseaacc qlhiaalmae ylklrnvhsw gaeafdkise nisrdecslk
     1741 ldagvqdihy neyilleqle vcadmlekae rfellghlyr livpmyeekr nyealancys
     1801 hlaqacnkiv evtksgkrll grfyrvaffg sayfeeengq eyiykepkit slseiserlh
     1861 hlysekfgae nvkmimdsvp iditeldpki syiqvthvta yfekfeletr qtefeqnhni
     1921 ssfmfetpft kegkargspe eqwkrrtilk tqysfpyikk rilviqkrim elspievald
     1981 emrqrvqele dvaligptdv kklqlrlqgs icvtvnagpl ayasafldpa lspqypddkv
     2041 eelkdvfref vkicytalqi nsklitsdqh eyqevlreny qklcqnlssl lgepiwpdeq
     2101 vgnfkrnsaa lfnvisgans htsta
//
LOCUS       XP_034194791            2123 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis protein Ziz isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194791
VERSION     XP_034194791.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338900.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194791.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2123
                     /product="dedicator of cytokinesis protein Ziz isoform X4"
                     /calculated_mol_wt=242773
     Region          36..147
                     /region_name="DOCK_C-D_N"
                     /note="Dedicator of cytokinesis C/D, N terminal;
                     pfam11878"
                     /db_xref="CDD:463380"
     Region          159..286
                     /region_name="PH_DOCK-D"
                     /note="Dedicator of cytokinesis-D subfamily Pleckstrin
                     homology (PH) domain; cd13267"
                     /db_xref="CDD:270087"
     Region          645..835
                     /region_name="C2_Dock-D"
                     /note="C2 domains found in Dedicator Of CytoKinesis (Dock)
                     class C proteins; cd08697"
                     /db_xref="CDD:176079"
     Region          1665..2085
                     /region_name="DHR2_DOCK_D"
                     /note="Dock Homology Region 2, a GEF domain, of Class D
                     Dedicator of Cytokinesis proteins; cd11694"
                     /db_xref="CDD:212567"
     Site            order(1778,1788,1790..1791,1793..1794,1797..1798,
                     1800..1801,1804..1805)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            order(1812..1815,1846..1847,1849..1852,1854..1855,
                     1874..1876,1893,1895,1924,1926,1933..1934,1940,1942,
                     1979..1980,1983,1997,1999,2003,2006..2008,2011..2017,2020,
                     2048,2066..2067,2069..2070,2073)
                     /site_type="other"
                     /note="Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            2011..2016
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212567"
     CDS             1..2123
                     /gene="Ziz"
                     /coded_by="XM_034338900.2:367..6738"
                     /db_xref="GeneID:117610997"
ORIGIN      
        1 mserkftrgl gkpgmaaqlr etvsqvvres tvqnkphlve pidfesfvlk nktllqndpq
       61 relllypqdd vsqvvlprry rtlvptvsqt sdneegeenl ltkeclqsyt snwnlihyky
      121 saysgsylel pkiakaddlk eevyeidtev dqvdeeltks ngitkegylm kgpeigssdr
      181 mfanigsksf krrfchlrqe vdgtyilelf kdekkgeakl tivmdfctev vrnpkrgryc
      241 felrmsgthk sytlaadnet dmqdwllkls svlqhykqqe ekraaslera cntpppspqp
      301 mqvygtlkgl eqsmnpqlik ysretdtsia larrenrkrl fniypniphs kqqsgnssel
      361 nidpykeqfg qrifvkcesl kfrlqapide keslcqvepy qttlslydar hgrkltenfh
      421 fdinhevvqg makelspigi mtestedvkl pndlksipld wikypkqaif sisnphpdif
      481 lvvridkilq gnicqtsepy lratkdprlg lkvhkqvrac cqrlgnyrmp fawaarplfr
      541 lysneldvss dfpaiyrqeg nkikddellk llseyrkpek lskltvipgw lkikiesite
      601 lpdntlstsl aalkpfplpp tseptleiae festsekdvh pyttyinhly vypqtlcfda
      661 qkiftrarni acivelrddd senaaplrci ygrpgspllc lrascavlhh navpswyeei
      721 kirlptklhs khhllfsfyh iscdmnkkke ngvescvgya wspllhkgrl nvdmdmnvqi
      781 lpvathlppg ylsiqplglg kgnagpeivw idsqrpvftv afqlistvft rdvhlhnlfa
      841 hmerildmki gavpadsetc kilkaahavq lvtiitflpt ilnqlfallt yttneevgly
      901 iirvlihfin mvhdagrket lqayikfvfv ppsqgngsit vheqlgkhlp tllqpnntdf
      961 lvvnkfmhhs nfffeimiks maqhllstgr ikmhrnerfs keyhekirnl vevimpylmn
     1021 kykempveth elnkslaqfl krcltfmdrg fvfrlinlyv dnfspgdqrt lhdfkftflq
     1081 iicshehyvs fnlpmmqsri tsrdlmneyc lsedfckhhf lvgllmqevk tslneivqir
     1141 kvaiatlrdl makhelddry qnkgqlsria fiyipwlgiv lenlhrlqsv ydngkteikq
     1201 ngtdristss sflankdtas nittvgtpks vhrltihldt qspirasmhl rdstyfaaia
     1261 gqglvngysc tsiesdtstm sgasqsnvsq ettiirepie ngtnekkrhs rslsvtqssp
     1321 rcdklqssev kdillcflfv ikylgdhqii awlqqcsdce mlsfftviem slhhfkyvgk
     1381 rqistnians sgkprtakam tlparmappd fstdapatst lqphntivre nlvetdggkv
     1441 hqaileanma tevglialdc lglfcihfkd vllatdgdnp imqkvfsiyl sflqvgqset
     1501 llrhvfasfr aflnnysiil fqgnavlcgr lcyellrccn sklscirqes callyllmrs
     1561 nfeftsrkgl trvhlqviis vsqmlgnvig lnnsrfqesl slinsyassd kvmkgtgfpv
     1621 evkdlnkrir tvlmataqmr ehnndpemlv dlqhslansy astpelrhtw letmarnhar
     1681 dgnfseaacc qlhiaalmae ylklrnvhsw gaeafdkise nisrdecslk ldageicmfc
     1741 tcvqdihyne yilleqlevc admlekaerf ellghlyrli vpmyeekrny ealancyshl
     1801 aqacnkivev tksgkrllgr fyrvaffgsa yfeeengqey iykepkitsl seiserlhhl
     1861 ysekfgaenv kmimdsvpid iteldpkisy iqvthvtayf ekfeletrqt efeqnhniss
     1921 fmfetpftke gkargspeeq wkrrtilktq ysfpyikkri lviqkrimel spievaldem
     1981 rqrvqeledv aligptdvkk lqlrlqgsic vtvnagplay asafldpals pqypddkvee
     2041 lkdvfrefvk icytalqins klitsdqhey qevlrenyqk lcqnlssllg epiwpdeqvg
     2101 nfkrnsaalf nvisgansht sta
//
LOCUS       XP_076546712            2118 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis protein Ziz isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546712
VERSION     XP_076546712.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690597.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2118
                     /product="dedicator of cytokinesis protein Ziz isoform X5"
                     /calculated_mol_wt=242187
     Region          36..147
                     /region_name="DOCK_C-D_N"
                     /note="Dedicator of cytokinesis C/D, N terminal;
                     pfam11878"
                     /db_xref="CDD:463380"
     Region          159..286
                     /region_name="PH_DOCK-D"
                     /note="Dedicator of cytokinesis-D subfamily Pleckstrin
                     homology (PH) domain; cd13267"
                     /db_xref="CDD:270087"
     Region          645..835
                     /region_name="C2_Dock-D"
                     /note="C2 domains found in Dedicator Of CytoKinesis (Dock)
                     class C proteins; cd08697"
                     /db_xref="CDD:176079"
     Region          1665..2080
                     /region_name="DHR2_DOCK_D"
                     /note="Dock Homology Region 2, a GEF domain, of Class D
                     Dedicator of Cytokinesis proteins; cd11694"
                     /db_xref="CDD:212567"
     Site            order(1773,1783,1785..1786,1788..1789,1792..1793,
                     1795..1796,1799..1800)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            order(1807..1810,1841..1842,1844..1847,1849..1850,
                     1869..1871,1888,1890,1919,1921,1928..1929,1935,1937,
                     1974..1975,1978,1992,1994,1998,2001..2003,2006..2012,2015,
                     2043,2061..2062,2064..2065,2068)
                     /site_type="other"
                     /note="Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            2006..2011
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212567"
     CDS             1..2118
                     /gene="Ziz"
                     /coded_by="XM_076690597.1:367..6723"
                     /db_xref="GeneID:117610997"
ORIGIN      
        1 mserkftrgl gkpgmaaqlr etvsqvvres tvqnkphlve pidfesfvlk nktllqndpq
       61 relllypqdd vsqvvlprry rtlvptvsqt sdneegeenl ltkeclqsyt snwnlihyky
      121 saysgsylel pkiakaddlk eevyeidtev dqvdeeltks ngitkegylm kgpeigssdr
      181 mfanigsksf krrfchlrqe vdgtyilelf kdekkgeakl tivmdfctev vrnpkrgryc
      241 felrmsgthk sytlaadnet dmqdwllkls svlqhykqqe ekraaslera cntpppspqp
      301 mqvygtlkgl eqsmnpqlik ysretdtsia larrenrkrl fniypniphs kqqsgnssel
      361 nidpykeqfg qrifvkcesl kfrlqapide keslcqvepy qttlslydar hgrkltenfh
      421 fdinhevvqg makelspigi mtestedvkl pndlksipld wikypkqaif sisnphpdif
      481 lvvridkilq gnicqtsepy lratkdprlg lkvhkqvrac cqrlgnyrmp fawaarplfr
      541 lysneldvss dfpaiyrqeg nkikddellk llseyrkpek lskltvipgw lkikiesite
      601 lpdntlstsl aalkpfplpp tseptleiae festsekdvh pyttyinhly vypqtlcfda
      661 qkiftrarni acivelrddd senaaplrci ygrpgspllc lrascavlhh navpswyeei
      721 kirlptklhs khhllfsfyh iscdmnkkke ngvescvgya wspllhkgrl nvdmdmnvqi
      781 lpvathlppg ylsiqplglg kgnagpeivw idsqrpvftv afqlistvft rdvhlhnlfa
      841 hmerildmki gavpadsetc kilkaahavq lvtiitflpt ilnqlfallt yttneevgly
      901 iirvlihfin mvhdagrket lqayikfvfv ppsqgngsit vheqlgkhlp tllqpnntdf
      961 lvvnkfmhhs nfffeimiks maqhllstgr ikmhrnerfs keyhekirnl vevimpylmn
     1021 kykempveth elnkslaqfl krcltfmdrg fvfrlinlyv dnfspgdqrt lhdfkftflq
     1081 iicshehyvs fnlpmmqsri tsrdlmneyc lsedfckhhf lvgllmqevk tslneivqir
     1141 kvaiatlrdl makhelddry qnkgqlsria fiyipwlgiv lenlhrlqsv ydngkteikq
     1201 ngtdristss sflankdtas nittvgtpks vhrltihldt qspirasmhl rdstyfaaia
     1261 gqglvngysc tsiesdtstm sgasqsnvsq ettiirepie ngtnekkrhs rslsvtqssp
     1321 rcdklqssev kdillcflfv ikylgdhqii awlqqcsdce mlsfftviem slhhfkyvgk
     1381 rqistnians sgkprtakam tlparmappd fstdapatst lqphntivre nlvetdggkv
     1441 hqaileanma tevglialdc lglfcihfkd vllatdgdnp imqkvfsiyl sflqvgqset
     1501 llrhvfasfr aflnnysiil fqgnavlcgr lcyellrccn sklscirqes callyllmrs
     1561 nfeftsrkgl trvhlqviis vsqmlgnvig lnnsrfqesl slinsyassd kvmkgtgfpv
     1621 evkdlnkrir tvlmataqmr ehnndpemlv dlqhslansy astpelrhtw letmarnhar
     1681 dgnfseaacc qlhiaalmae ylklrnvhsw gaeafdkise nisrdecslk ldageicvqd
     1741 ihyneyille qlevcadmle kaerfellgh lyrlivpmye ekrnyealan cyshlaqacn
     1801 kivevtksgk rllgrfyrva ffgsayfeee ngqeyiykep kitslseise rlhhlysekf
     1861 gaenvkmimd svpiditeld pkisyiqvth vtayfekfel etrqtefeqn hnissfmfet
     1921 pftkegkarg speeqwkrrt ilktqysfpy ikkrilviqk rimelspiev aldemrqrvq
     1981 eledvaligp tdvkklqlrl qgsicvtvna gplayasafl dpalspqypd dkveelkdvf
     2041 refvkicyta lqinsklits dqheyqevlr enyqklcqnl ssllgepiwp deqvgnfkrn
     2101 saalfnvisg anshtsta
//
LOCUS       XP_034194796            2115 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis protein Ziz isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194796
VERSION     XP_034194796.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338905.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194796.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2115
                     /product="dedicator of cytokinesis protein Ziz isoform X6"
                     /calculated_mol_wt=241842
     Region          36..147
                     /region_name="DOCK_C-D_N"
                     /note="Dedicator of cytokinesis C/D, N terminal;
                     pfam11878"
                     /db_xref="CDD:463380"
     Region          159..286
                     /region_name="PH_DOCK-D"
                     /note="Dedicator of cytokinesis-D subfamily Pleckstrin
                     homology (PH) domain; cd13267"
                     /db_xref="CDD:270087"
     Region          645..835
                     /region_name="C2_Dock-D"
                     /note="C2 domains found in Dedicator Of CytoKinesis (Dock)
                     class C proteins; cd08697"
                     /db_xref="CDD:176079"
     Region          1665..2077
                     /region_name="DHR2_DOCK_D"
                     /note="Dock Homology Region 2, a GEF domain, of Class D
                     Dedicator of Cytokinesis proteins; cd11694"
                     /db_xref="CDD:212567"
     Site            order(1770,1780,1782..1783,1785..1786,1789..1790,
                     1792..1793,1796..1797)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            order(1804..1807,1838..1839,1841..1844,1846..1847,
                     1866..1868,1885,1887,1916,1918,1925..1926,1932,1934,
                     1971..1972,1975,1989,1991,1995,1998..2000,2003..2009,2012,
                     2040,2058..2059,2061..2062,2065)
                     /site_type="other"
                     /note="Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212567"
     Site            2003..2008
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212567"
     CDS             1..2115
                     /gene="Ziz"
                     /coded_by="XM_034338905.2:367..6714"
                     /db_xref="GeneID:117610997"
ORIGIN      
        1 mserkftrgl gkpgmaaqlr etvsqvvres tvqnkphlve pidfesfvlk nktllqndpq
       61 relllypqdd vsqvvlprry rtlvptvsqt sdneegeenl ltkeclqsyt snwnlihyky
      121 saysgsylel pkiakaddlk eevyeidtev dqvdeeltks ngitkegylm kgpeigssdr
      181 mfanigsksf krrfchlrqe vdgtyilelf kdekkgeakl tivmdfctev vrnpkrgryc
      241 felrmsgthk sytlaadnet dmqdwllkls svlqhykqqe ekraaslera cntpppspqp
      301 mqvygtlkgl eqsmnpqlik ysretdtsia larrenrkrl fniypniphs kqqsgnssel
      361 nidpykeqfg qrifvkcesl kfrlqapide keslcqvepy qttlslydar hgrkltenfh
      421 fdinhevvqg makelspigi mtestedvkl pndlksipld wikypkqaif sisnphpdif
      481 lvvridkilq gnicqtsepy lratkdprlg lkvhkqvrac cqrlgnyrmp fawaarplfr
      541 lysneldvss dfpaiyrqeg nkikddellk llseyrkpek lskltvipgw lkikiesite
      601 lpdntlstsl aalkpfplpp tseptleiae festsekdvh pyttyinhly vypqtlcfda
      661 qkiftrarni acivelrddd senaaplrci ygrpgspllc lrascavlhh navpswyeei
      721 kirlptklhs khhllfsfyh iscdmnkkke ngvescvgya wspllhkgrl nvdmdmnvqi
      781 lpvathlppg ylsiqplglg kgnagpeivw idsqrpvftv afqlistvft rdvhlhnlfa
      841 hmerildmki gavpadsetc kilkaahavq lvtiitflpt ilnqlfallt yttneevgly
      901 iirvlihfin mvhdagrket lqayikfvfv ppsqgngsit vheqlgkhlp tllqpnntdf
      961 lvvnkfmhhs nfffeimiks maqhllstgr ikmhrnerfs keyhekirnl vevimpylmn
     1021 kykempveth elnkslaqfl krcltfmdrg fvfrlinlyv dnfspgdqrt lhdfkftflq
     1081 iicshehyvs fnlpmmqsri tsrdlmneyc lsedfckhhf lvgllmqevk tslneivqir
     1141 kvaiatlrdl makhelddry qnkgqlsria fiyipwlgiv lenlhrlqsv ydngkteikq
     1201 ngtdristss sflankdtas nittvgtpks vhrltihldt qspirasmhl rdstyfaaia
     1261 gqglvngysc tsiesdtstm sgasqsnvsq ettiirepie ngtnekkrhs rslsvtqssp
     1321 rcdklqssev kdillcflfv ikylgdhqii awlqqcsdce mlsfftviem slhhfkyvgk
     1381 rqistnians sgkprtakam tlparmappd fstdapatst lqphntivre nlvetdggkv
     1441 hqaileanma tevglialdc lglfcihfkd vllatdgdnp imqkvfsiyl sflqvgqset
     1501 llrhvfasfr aflnnysiil fqgnavlcgr lcyellrccn sklscirqes callyllmrs
     1561 nfeftsrkgl trvhlqviis vsqmlgnvig lnnsrfqesl slinsyassd kvmkgtgfpv
     1621 evkdlnkrir tvlmataqmr ehnndpemlv dlqhslansy astpelrhtw letmarnhar
     1681 dgnfseaacc qlhiaalmae ylklrnvhsw gaeafdkise nisrdecslk ldagvqdihy
     1741 neyilleqle vcadmlekae rfellghlyr livpmyeekr nyealancys hlaqacnkiv
     1801 evtksgkrll grfyrvaffg sayfeeengq eyiykepkit slseiserlh hlysekfgae
     1861 nvkmimdsvp iditeldpki syiqvthvta yfekfeletr qtefeqnhni ssfmfetpft
     1921 kegkargspe eqwkrrtilk tqysfpyikk rilviqkrim elspievald emrqrvqele
     1981 dvaligptdv kklqlrlqgs icvtvnagpl ayasafldpa lspqypddkv eelkdvfref
     2041 vkicytalqi nsklitsdqh eyqevlreny qklcqnlssl lgepiwpdeq vgnfkrnsaa
     2101 lfnvisgans htsta
//
LOCUS       XP_034196043            1164 aa            linear   INV 03-SEP-2025
DEFINITION  blastoderm-specific gene 25D [Osmia lignaria lignaria].
ACCESSION   XP_034196043
VERSION     XP_034196043.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340152.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196043.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1164
                     /product="blastoderm-specific gene 25D"
                     /calculated_mol_wt=134935
     Region          142..194
                     /region_name="EF-hand_8"
                     /note="EF-hand domain pair; pfam13833"
                     /db_xref="CDD:404678"
     Region          259..>885
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <276..>496
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1164
                     /gene="Bsg25D"
                     /coded_by="XM_034340152.2:197..3691"
                     /db_xref="GeneID:117611816"
ORIGIN      
        1 mdehnndpye qqlyavfqsc ltkgetelqe dnwlslchkl hlteqsdelr sciqqqkkek
       61 qsisfqefrt allmlldksq ekdtitesqc dlslnavdnk rgnlllpnni ksnvdflsir
      121 tgvpvdenrl kclwekvnic lkenvdsati yficnclgip alpkqiiqci fekldqncdg
      181 lisleeflvi fqsrtpveeq ltldknneta keltkldfrk rnfdvhtsrk tsftrnasfl
      241 dtwklsnian tslltdgglk sseisladlt atlcdelknf ndsldhsair shimllrdvl
      301 ilyqeelhkl nlvienvsge keklrtdiie anerantlaq eideqhvrqe eivqnlrkql
      361 eqrhaetlqe lsnqlssere mnatalkskd eqiqmsqken heirnkfvna lqenqvlese
      421 nenlrdqiek lkqsntellt qikmlaaehd esqnievkhq qeviylidri khlqseavll
      481 rdqndeltae leslkqydch akdirqnnll gahttrssiq sdktenskea fvvdedeldv
      541 flkhstptfc nsneednmqn ckkidlknfk divikqlknv llsnnkctle ncslkneise
      601 iitrlqsnvn sqggkeidfv ksiasemete ceertteslr dfvvskhkhs ipvkraiisn
      661 nndnnydidn lnnrdiksls qnkvsslrdy pprkeectnv dhsksnkekd tlliqstkvs
      721 neisskinss ltkmnessed vvaflnlkiq eseaahaaem kqlteqcael ernldllkie
      781 yeecedfwtg kleeerqlfe qeqkisdekf seliakmaey eelispvdka kngrlspiee
      841 kfnleqqyld leeefekwkg qmeeeitqkd keiknlhekl ksferpimvd isvqvtdeft
      901 fssllarsny vpsdsfniqs ynelssklaq cndnihklpd efkynriles snlsndfipr
      961 ltkeevdvsn cdpyemhtqy svksksgtck mekpelqwel tevdgklyke enipdteydv
     1021 iqntlklrnl nvsncqcmrn daqmccvnvs ilrnlnaklq tqerkkhqlq ecfkqqqyhi
     1081 ehvlqyiisq hqvevselqc llrntqerlq iefqtsaeqa eqlartdilv kdlyienacl
     1141 tanlkrlqqh yhiltglnae stsi
//
LOCUS       XP_034196044            1164 aa            linear   INV 03-SEP-2025
DEFINITION  blastoderm-specific gene 25D [Osmia lignaria lignaria].
ACCESSION   XP_034196044
VERSION     XP_034196044.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340153.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196044.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1164
                     /product="blastoderm-specific gene 25D"
                     /calculated_mol_wt=134935
     Region          142..194
                     /region_name="EF-hand_8"
                     /note="EF-hand domain pair; pfam13833"
                     /db_xref="CDD:404678"
     Region          259..>885
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <276..>496
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1164
                     /gene="Bsg25D"
                     /coded_by="XM_034340153.2:175..3669"
                     /db_xref="GeneID:117611816"
ORIGIN      
        1 mdehnndpye qqlyavfqsc ltkgetelqe dnwlslchkl hlteqsdelr sciqqqkkek
       61 qsisfqefrt allmlldksq ekdtitesqc dlslnavdnk rgnlllpnni ksnvdflsir
      121 tgvpvdenrl kclwekvnic lkenvdsati yficnclgip alpkqiiqci fekldqncdg
      181 lisleeflvi fqsrtpveeq ltldknneta keltkldfrk rnfdvhtsrk tsftrnasfl
      241 dtwklsnian tslltdgglk sseisladlt atlcdelknf ndsldhsair shimllrdvl
      301 ilyqeelhkl nlvienvsge keklrtdiie anerantlaq eideqhvrqe eivqnlrkql
      361 eqrhaetlqe lsnqlssere mnatalkskd eqiqmsqken heirnkfvna lqenqvlese
      421 nenlrdqiek lkqsntellt qikmlaaehd esqnievkhq qeviylidri khlqseavll
      481 rdqndeltae leslkqydch akdirqnnll gahttrssiq sdktenskea fvvdedeldv
      541 flkhstptfc nsneednmqn ckkidlknfk divikqlknv llsnnkctle ncslkneise
      601 iitrlqsnvn sqggkeidfv ksiasemete ceertteslr dfvvskhkhs ipvkraiisn
      661 nndnnydidn lnnrdiksls qnkvsslrdy pprkeectnv dhsksnkekd tlliqstkvs
      721 neisskinss ltkmnessed vvaflnlkiq eseaahaaem kqlteqcael ernldllkie
      781 yeecedfwtg kleeerqlfe qeqkisdekf seliakmaey eelispvdka kngrlspiee
      841 kfnleqqyld leeefekwkg qmeeeitqkd keiknlhekl ksferpimvd isvqvtdeft
      901 fssllarsny vpsdsfniqs ynelssklaq cndnihklpd efkynriles snlsndfipr
      961 ltkeevdvsn cdpyemhtqy svksksgtck mekpelqwel tevdgklyke enipdteydv
     1021 iqntlklrnl nvsncqcmrn daqmccvnvs ilrnlnaklq tqerkkhqlq ecfkqqqyhi
     1081 ehvlqyiisq hqvevselqc llrntqerlq iefqtsaeqa eqlartdilv kdlyienacl
     1141 tanlkrlqqh yhiltglnae stsi
//
LOCUS       XP_034196045             927 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611818 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196045
VERSION     XP_034196045.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340154.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196045.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..927
                     /product="uncharacterized protein LOC117611818 isoform X1"
                     /calculated_mol_wt=104633
     Region          82..284
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(113,151,155,230,233..234,258)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            113
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..927
                     /gene="LOC117611818"
                     /coded_by="XM_034340154.2:332..3115"
                     /db_xref="GeneID:117611818"
ORIGIN      
        1 mmeltmedfn dgvdgklafr mrkvnpeqfh tlvkmhlsfe ldlntedssd cinektklkk
       61 wgllsfskkp kstinlqkgi eganlsedgi nqmkqlidyl skeqnimqeg ifrrtgkltr
      121 qqdlksslyq giplnlndgr ysvhdcasvl kgflaelpep llsdlhypah cqiaelcsse
      181 vnendarllr slqllllllp smnrillkhv lnllnktasf ecsnkmncdt latlftphlm
      241 cprklspeal hinsqnlscl vafmikkase lfeippklat diraywveqe rkllspkrvd
      301 lnesipdttg tahtvfsfvd hkltakanst qdtqaalaql yahiqampes akkrrlvkqf
      361 nkengqgtpr qvkhcrtksl gdsikkhifq kkilghkkfi dinigcnitr ssseenilst
      421 dleksllqtk mllsksddel svdnsdtnne nllhtkhisn stgslniptl rnykekrram
      481 lmrfvnikee qldnsyhnyi mdepsksfqe nsytddsmrs ksdanislqe hfnieeseqd
      541 dntvrlsrqm seppdmyssq ttlhetsenq lirqnlrptl rtidgisssk gskdntnise
      601 qhteaesntd nacnlspivk nrlwnvyvah tstpsnllrk slvnndymlt pitnndksms
      661 pitqsatkmt kamqetmmtp rsrkpvmmvs gsnlcnlaiv nnswnqhsms nnirgsnldi
      721 igsmnytsil eesqkpnntd seiiygdivd senkenfnke lteqrklvss vpddcyavaq
      781 qstvsitstf reyllsrsvl taspvdlsft srtgdfeqse sdlnilnedg lsdsllccld
      841 gnqpesdtsg iisgstnsas nslekhdegt nsprkratsl qrndskrsir enrisnsire
      901 qgfkykqlse stlnsvkikd tfqetsf
//
LOCUS       XP_034196046             926 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611818 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196046
VERSION     XP_034196046.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340155.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196046.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..926
                     /product="uncharacterized protein LOC117611818 isoform X2"
                     /calculated_mol_wt=104546
     Region          81..283
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(112,150,154,229,232..233,257)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:238090"
     Site            112
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:238090"
     CDS             1..926
                     /gene="LOC117611818"
                     /coded_by="XM_034340155.2:331..3111"
                     /db_xref="GeneID:117611818"
ORIGIN      
        1 mmeltmedfn dgvdgklafr mrkvnpeqfh tlvkmhlsfe ldlntedsdc inektklkkw
       61 gllsfskkpk stinlqkgie ganlsedgin qmkqlidyls keqnimqegi frrtgkltrq
      121 qdlksslyqg iplnlndgry svhdcasvlk gflaelpepl lsdlhypahc qiaelcssev
      181 nendarllrs lqllllllps mnrillkhvl nllnktasfe csnkmncdtl atlftphlmc
      241 prklspealh insqnlsclv afmikkasel feippklatd iraywveqer kllspkrvdl
      301 nesipdttgt ahtvfsfvdh kltakanstq dtqaalaqly ahiqampesa kkrrlvkqfn
      361 kengqgtprq vkhcrtkslg dsikkhifqk kilghkkfid inigcnitrs sseenilstd
      421 leksllqtkm llsksddels vdnsdtnnen llhtkhisns tgslniptlr nykekrraml
      481 mrfvnikeeq ldnsyhnyim depsksfqen sytddsmrsk sdanislqeh fnieeseqdd
      541 ntvrlsrqms eppdmyssqt tlhetsenql irqnlrptlr tidgissskg skdntniseq
      601 hteaesntdn acnlspivkn rlwnvyvaht stpsnllrks lvnndymltp itnndksmsp
      661 itqsatkmtk amqetmmtpr srkpvmmvsg snlcnlaivn nswnqhsmsn nirgsnldii
      721 gsmnytsile esqkpnntds eiiygdivds enkenfnkel teqrklvssv pddcyavaqq
      781 stvsitstfr eyllsrsvlt aspvdlsfts rtgdfeqses dlnilnedgl sdsllccldg
      841 nqpesdtsgi isgstnsasn slekhdegtn sprkratslq rndskrsire nrisnsireq
      901 gfkykqlses tlnsvkikdt fqetsf
//
LOCUS       XP_034196047             845 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611819 [Osmia lignaria lignaria].
ACCESSION   XP_034196047
VERSION     XP_034196047.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340156.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..845
                     /product="uncharacterized protein LOC117611819"
                     /calculated_mol_wt=94806
     Region          641..806
                     /region_name="MEIOC"
                     /note="Meiosis-specific coiled-coil domain-containing
                     protein MEIOC; pfam15189"
                     /db_xref="CDD:434527"
     CDS             1..845
                     /gene="LOC117611819"
                     /coded_by="XM_034340156.2:142..2679"
                     /db_xref="GeneID:117611819"
ORIGIN      
        1 malsevhnla aiitnngftv sknnviecni nmleprnnyp adrcnaeyny wnydlmecnt
       61 qknmnveded sqdlnnpmvn dlvskilddd svvsdnaqns sysgniqyhs dsqccivenk
      121 menktdpnlt ninhfnihtt fnklhnnisn naklecnynn lceeiktlsl nrcagqvlde
      181 gnlysngvsa ysntyssdsq syilrpsddt ccingstgmp lcndlltttn vrnsnkqatn
      241 wsenlsasgc tsdlfgnyqr ienctnpdln fnmaltlald spdpvtlnei eyhhnigqng
      301 ttnaynnptp smhelysmta nnqnyrpssa mtdlsidsgf lsnsplqhfs padtnlhncf
      361 gnniqqrsna gdykdlhdss rlsmgnisip neqlqflqqq arsyklnqav dqeykqlidy
      421 ypgvsdftpp singldtnen clsnndyrid nnllkiispk sktietktys pigfpknlss
      481 rhthqsiaky gyhnyqqyta nngetlkmlp qsstsyhdlk qpntntntyk megfdltkei
      541 asfpsvshlt nqiaagssmn kdtfnylmkq rhhmpriqns hgipsadvlf nsgvvpnpst
      601 vrsgvfppvm pvpvplpvpr lfsvlygggl glrngsgaar rtapssilhf rleqtyeqfk
      661 qlekerkkce aglaahfpgk rvtsannipi prlqgnpsrv drliidylre harvitliak
      721 merlrgttmn qrvhkameyw leaikfvqec rkreianatk rqkenppcil vyndndiltl
      781 aasvyeltka sryartgmyn alqatllydl diekkvvess kdpvlvikrn esqvgsnagd
      841 ysnct
//
LOCUS       XP_034196049             726 aa            linear   INV 03-SEP-2025
DEFINITION  exonuclease tos isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196049
VERSION     XP_034196049.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340158.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..726
                     /product="exonuclease tos isoform X1"
                     /calculated_mol_wt=82842
     Region          1..202
                     /region_name="PIN_EXO1"
                     /note="FEN-like PIN domains of Exonuclease-1, a
                     structure-specific, divalent-metal-ion dependent, 5'
                     nuclease and homologs; cd09857"
                     /db_xref="CDD:350207"
     Site            order(2..4,7..8,30,32..33,36..37,40..41,54,58,78,85,89,92,
                     95..96,117,120..121,150,152,170..173,185)
                     /site_type="active"
                     /db_xref="CDD:350207"
     Site            order(84..109,111..127)
                     /site_type="other"
                     /note="helical arch"
                     /db_xref="CDD:350207"
     Region          214..291
                     /region_name="H3TH_EXO1"
                     /note="H3TH domain of Exonuclease-1, a structure-specific,
                     divalent-metal-ion dependent, 5' nuclease; cd09908"
                     /db_xref="CDD:188628"
     Site            order(223..228,230..235,237..240)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:188628"
     Site            226
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:188628"
     CDS             1..726
                     /gene="tos"
                     /coded_by="XM_034340158.2:376..2556"
                     /db_xref="GeneID:117611820"
ORIGIN      
        1 mgitgllpfl ekcsertnin kfsggtvaid tycwlhrgvf acadklsmgq qtkayinycm
       61 kqihmllayk ikpilvfdgr hlpakaqtea krrearetnr rkaaelmqmg qhaegrnllr
      121 rsvdithema lelikechkm nidcivapye adaqlaylni ngiadvvite dsdltlfgck
      181 kiffkmdayg ngllvqqdli hlamgvssad fsldefrymc ilsgcdylss lpgiglskar
      241 kfirtnkdcn ihraltrlgs ylnmkslvvt qeyrdafila ditfkhqlvf cplqrkqvrl
      301 nppmsdvtee qlyyagtets pdialqlayg ncdpftlkml hnfdpdktei lnsksnntwr
      361 qkmlhpqhis iwskkyvskq nllqsfiqkk dlttwpntvg keavlntnrl rnaclvieed
      421 hpdckelnqk eildiyedkn tkeiksnlei dsvpsdeeis pvlirrtnlf akqpsstkts
      481 psllfkhksr vkgrhimrlr rtivneevvt eskffakptn etdsnvvtnd ntlcptdnsn
      541 killekenin knesketndi klqalmitde vktdtyrmdv deehnasnec katkysnssf
      601 ncdlmtspvn vqisevlthk tninescnln ssltvsevdt dfdfliqqgd istlsnssfk
      661 wsdtrqcvqi nkkakcdkyr nsksrtvvnn trrsqqlcsv qrqqgllsmy gfekkififr
      721 igiksd
//
LOCUS       XP_034196050             718 aa            linear   INV 03-SEP-2025
DEFINITION  exonuclease tos isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196050
VERSION     XP_034196050.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340159.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..718
                     /product="exonuclease tos isoform X2"
                     /calculated_mol_wt=81864
     Region          1..202
                     /region_name="PIN_EXO1"
                     /note="FEN-like PIN domains of Exonuclease-1, a
                     structure-specific, divalent-metal-ion dependent, 5'
                     nuclease and homologs; cd09857"
                     /db_xref="CDD:350207"
     Site            order(2..4,7..8,30,32..33,36..37,40..41,54,58,78,85,89,92,
                     95..96,117,120..121,150,152,170..173,185)
                     /site_type="active"
                     /db_xref="CDD:350207"
     Site            order(84..109,111..127)
                     /site_type="other"
                     /note="helical arch"
                     /db_xref="CDD:350207"
     Region          214..291
                     /region_name="H3TH_EXO1"
                     /note="H3TH domain of Exonuclease-1, a structure-specific,
                     divalent-metal-ion dependent, 5' nuclease; cd09908"
                     /db_xref="CDD:188628"
     Site            order(223..228,230..235,237..240)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:188628"
     Site            226
                     /site_type="other"
                     /note="putative metal binding site [ion binding]"
                     /db_xref="CDD:188628"
     CDS             1..718
                     /gene="tos"
                     /coded_by="XM_034340159.2:376..2532"
                     /db_xref="GeneID:117611820"
ORIGIN      
        1 mgitgllpfl ekcsertnin kfsggtvaid tycwlhrgvf acadklsmgq qtkayinycm
       61 kqihmllayk ikpilvfdgr hlpakaqtea krrearetnr rkaaelmqmg qhaegrnllr
      121 rsvdithema lelikechkm nidcivapye adaqlaylni ngiadvvite dsdltlfgck
      181 kiffkmdayg ngllvqqdli hlamgvssad fsldefrymc ilsgcdylss lpgiglskar
      241 kfirtnkdcn ihraltrlgs ylnmkslvvt qeyrdafila ditfkhqlvf cplqrkqvrl
      301 nppmsdvtee qlyyagtets pdialqlayg ncdpftlkml hnfdpdktei lnsksnntwr
      361 qkmlhpqhis iwskkyvskq nllqsfiqkk dlttwpntvg keavlntnrl rnaclvieed
      421 hpdckelnqk eildiyedkn tkeiksnlei dsvpsdeeis pvlirrtnlf akqpsstkts
      481 psllfkhksr vkgrhimrlr rtivneevvt eskffakptn etdsnvvtnd ntlcptdnsn
      541 killekenin knesketndi klqalmitde vktdtyrmdv deehnasnec katkysnssf
      601 ncdlmtspvn vqisevlthk tninescnln ssltvsevdt dfdfliqqgd istlsnssfk
      661 wsdtrqcvqi nkkakcdkyr nsksrtvvnn trrsqqlcsv qrqqgllsmy gfekkkla
//
LOCUS       XP_034196051             495 aa            linear   INV 03-SEP-2025
DEFINITION  major facilitator superfamily transporter 9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196051
VERSION     XP_034196051.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340160.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..495
                     /product="major facilitator superfamily transporter 9"
                     /calculated_mol_wt=55279
     Region          42..464
                     /region_name="MFS_SLC17"
                     /note="Solute carrier 17 (SLC17) family of the Major
                     Facilitator Superfamily of transporters; cd17318"
                     /db_xref="CDD:340876"
     Site            order(50..51,54..55,58,106,158..159,161..163,166,186,
                     189..190,193,285,288..289,292..294,297,325,329,387..388,
                     392,396,412,415..416,419..420,423)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340876"
     CDS             1..495
                     /gene="MFS9"
                     /coded_by="XM_034340160.2:233..1720"
                     /db_xref="GeneID:117611821"
ORIGIN      
        1 mkgrkssves akdteygtvk yedemasswr fwkkrrhvvg alaflgffts yilrvnlsva
       61 ivamvkaevr kvdengnvyy ekefdwdsqm qgvllssffy gyictqlfgg wlgariggkr
      121 vfgigiaita fltiitppla kisyniflal rivegicegv typcihaiws hwapplersk
      181 latfafsgsf fgtvfampva glmveylgwe svfyvfggvg lvwfflwwii vkdkpeedph
      241 iskaeleyim cslgaskteg tsqkithpwk smltsppvwa iiaahfsenw gfytmltqlp
      301 tfmndvlnfk ldktgylsal pylamtiavq fsgyladylr tkkiltttqv rklfncgafa
      361 fqtifmlcaa filtpagvvt citfgiglgg fawsgfgvnh ldiapqhasv lmgigntiat
      421 lpgvvsplit gyivrnksts ewrvifiits viyligaviy glfasgekqr waeenkekkr
      481 akqfyenpam evdnl
//
LOCUS       XP_034196054             484 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase 1L [Osmia lignaria lignaria].
ACCESSION   XP_034196054
VERSION     XP_034196054.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340163.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="protein phosphatase 1L"
                     /calculated_mol_wt=54047
     Region          120..470
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(130,134..135,155..157,421,461)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..484
                     /gene="LOC117611822"
                     /coded_by="XM_034340163.2:192..1646"
                     /db_xref="GeneID:117611822"
ORIGIN      
        1 mddeledril yqtyvshmkl vskfavgipt glnagintpl gyfwriiray alkpevlicg
       61 vvlavvlfyi qavdlwsrsl lgkiqytlgr ttskvsklqf lvndsvnngv klswelkqgy
      121 iaayavqghr armedrfvvn edmnntgvsl favfdghgge faanyardkl ipninrkvie
      181 lkdliagkap rvpedtdkie epeqkeeknd tgiperkksf rktvstsltd dcmkknvgvt
      241 dpelldklds lsrpitrevr pcrttekptk vditnyldgn kinygrlltd evlavdrllv
      301 eaakknmdva gttalialle dnklivanvg dsrgvmcdgk gnaiplsfdh kpqqererkr
      361 inkagglvtf ngvwrvagil atsralgdyp lkdkkfviad pdiltfdlsd hnpmfivlas
      421 dglwdtftne eavafikeri nephfgaksi tlqsyyrgsa dnitvvvinl kdrkysiset
      481 kknq
//
LOCUS       XP_076546713             484 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase 1L [Osmia lignaria lignaria].
ACCESSION   XP_076546713
VERSION     XP_076546713.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690598.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="protein phosphatase 1L"
                     /calculated_mol_wt=54047
     Region          120..470
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(130,134..135,155..157,421,461)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..484
                     /gene="LOC117611822"
                     /coded_by="XM_076690598.1:156..1610"
                     /db_xref="GeneID:117611822"
ORIGIN      
        1 mddeledril yqtyvshmkl vskfavgipt glnagintpl gyfwriiray alkpevlicg
       61 vvlavvlfyi qavdlwsrsl lgkiqytlgr ttskvsklqf lvndsvnngv klswelkqgy
      121 iaayavqghr armedrfvvn edmnntgvsl favfdghgge faanyardkl ipninrkvie
      181 lkdliagkap rvpedtdkie epeqkeeknd tgiperkksf rktvstsltd dcmkknvgvt
      241 dpelldklds lsrpitrevr pcrttekptk vditnyldgn kinygrlltd evlavdrllv
      301 eaakknmdva gttalialle dnklivanvg dsrgvmcdgk gnaiplsfdh kpqqererkr
      361 inkagglvtf ngvwrvagil atsralgdyp lkdkkfviad pdiltfdlsd hnpmfivlas
      421 dglwdtftne eavafikeri nephfgaksi tlqsyyrgsa dnitvvvinl kdrkysiset
      481 kknq
//
LOCUS       XP_076546714             484 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase 1L [Osmia lignaria lignaria].
ACCESSION   XP_076546714
VERSION     XP_076546714.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690599.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="protein phosphatase 1L"
                     /calculated_mol_wt=54047
     Region          120..470
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(130,134..135,155..157,421,461)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..484
                     /gene="LOC117611822"
                     /coded_by="XM_076690599.1:193..1647"
                     /db_xref="GeneID:117611822"
ORIGIN      
        1 mddeledril yqtyvshmkl vskfavgipt glnagintpl gyfwriiray alkpevlicg
       61 vvlavvlfyi qavdlwsrsl lgkiqytlgr ttskvsklqf lvndsvnngv klswelkqgy
      121 iaayavqghr armedrfvvn edmnntgvsl favfdghgge faanyardkl ipninrkvie
      181 lkdliagkap rvpedtdkie epeqkeeknd tgiperkksf rktvstsltd dcmkknvgvt
      241 dpelldklds lsrpitrevr pcrttekptk vditnyldgn kinygrlltd evlavdrllv
      301 eaakknmdva gttalialle dnklivanvg dsrgvmcdgk gnaiplsfdh kpqqererkr
      361 inkagglvtf ngvwrvagil atsralgdyp lkdkkfviad pdiltfdlsd hnpmfivlas
      421 dglwdtftne eavafikeri nephfgaksi tlqsyyrgsa dnitvvvinl kdrkysiset
      481 kknq
//
LOCUS       XP_034196055             484 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase 1L [Osmia lignaria lignaria].
ACCESSION   XP_034196055
VERSION     XP_034196055.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340164.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="protein phosphatase 1L"
                     /calculated_mol_wt=54047
     Region          120..470
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(130,134..135,155..157,421,461)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..484
                     /gene="LOC117611822"
                     /coded_by="XM_034340164.2:205..1659"
                     /db_xref="GeneID:117611822"
ORIGIN      
        1 mddeledril yqtyvshmkl vskfavgipt glnagintpl gyfwriiray alkpevlicg
       61 vvlavvlfyi qavdlwsrsl lgkiqytlgr ttskvsklqf lvndsvnngv klswelkqgy
      121 iaayavqghr armedrfvvn edmnntgvsl favfdghgge faanyardkl ipninrkvie
      181 lkdliagkap rvpedtdkie epeqkeeknd tgiperkksf rktvstsltd dcmkknvgvt
      241 dpelldklds lsrpitrevr pcrttekptk vditnyldgn kinygrlltd evlavdrllv
      301 eaakknmdva gttalialle dnklivanvg dsrgvmcdgk gnaiplsfdh kpqqererkr
      361 inkagglvtf ngvwrvagil atsralgdyp lkdkkfviad pdiltfdlsd hnpmfivlas
      421 dglwdtftne eavafikeri nephfgaksi tlqsyyrgsa dnitvvvinl kdrkysiset
      481 kknq
//
LOCUS       XP_034196056             484 aa            linear   INV 03-SEP-2025
DEFINITION  protein phosphatase 1L [Osmia lignaria lignaria].
ACCESSION   XP_034196056
VERSION     XP_034196056.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340165.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="protein phosphatase 1L"
                     /calculated_mol_wt=54047
     Region          120..470
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(130,134..135,155..157,421,461)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..484
                     /gene="LOC117611822"
                     /coded_by="XM_034340165.2:74..1528"
                     /db_xref="GeneID:117611822"
ORIGIN      
        1 mddeledril yqtyvshmkl vskfavgipt glnagintpl gyfwriiray alkpevlicg
       61 vvlavvlfyi qavdlwsrsl lgkiqytlgr ttskvsklqf lvndsvnngv klswelkqgy
      121 iaayavqghr armedrfvvn edmnntgvsl favfdghgge faanyardkl ipninrkvie
      181 lkdliagkap rvpedtdkie epeqkeeknd tgiperkksf rktvstsltd dcmkknvgvt
      241 dpelldklds lsrpitrevr pcrttekptk vditnyldgn kinygrlltd evlavdrllv
      301 eaakknmdva gttalialle dnklivanvg dsrgvmcdgk gnaiplsfdh kpqqererkr
      361 inkagglvtf ngvwrvagil atsralgdyp lkdkkfviad pdiltfdlsd hnpmfivlas
      421 dglwdtftne eavafikeri nephfgaksi tlqsyyrgsa dnitvvvinl kdrkysiset
      481 kknq
//
LOCUS       XP_034196057             386 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 4a isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196057
VERSION     XP_034196057.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340166.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="autophagy-related 4a isoform X1"
                     /calculated_mol_wt=43298
     Region          38..323
                     /region_name="Peptidase_C54"
                     /note="Peptidase family C54; pfam03416"
                     /db_xref="CDD:460915"
     CDS             1..386
                     /gene="Atg4a"
                     /coded_by="XM_034340166.2:289..1449"
                     /db_xref="GeneID:117611823"
ORIGIN      
        1 mkmdslftqn isdepedipq tdepvwilgk kysaikelda irrdirsklw ftyrkgfvpi
       61 ggytstftsd kgwgcmlrcg qmvlghalii lhlgrdwqwt petrnstylk ilerfedrrt
      121 apfsihqias mgasegkevg qwfgpntiaq vlkklviyde wssitihval dntlivndil
      181 rqcrveggtt aeadgniplk apsqwkplll liplrlglse inpiyinglk nsfkipqslg
      241 viggkpnlal yfigcvgnev iyldphttqr sgsvdkkvee deiemdatyh ckfasripit
      301 gmdpsvalcf fcatekdfkt lckiiqeeli tpekqpllel caerlahwsp aedvaaseai
      361 aapsfvdfeh idpqydtsde dfellg
//
LOCUS       XP_034196058             386 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 4a isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196058
VERSION     XP_034196058.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340167.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="autophagy-related 4a isoform X1"
                     /calculated_mol_wt=43298
     Region          38..323
                     /region_name="Peptidase_C54"
                     /note="Peptidase family C54; pfam03416"
                     /db_xref="CDD:460915"
     CDS             1..386
                     /gene="Atg4a"
                     /coded_by="XM_034340167.2:303..1463"
                     /db_xref="GeneID:117611823"
ORIGIN      
        1 mkmdslftqn isdepedipq tdepvwilgk kysaikelda irrdirsklw ftyrkgfvpi
       61 ggytstftsd kgwgcmlrcg qmvlghalii lhlgrdwqwt petrnstylk ilerfedrrt
      121 apfsihqias mgasegkevg qwfgpntiaq vlkklviyde wssitihval dntlivndil
      181 rqcrveggtt aeadgniplk apsqwkplll liplrlglse inpiyinglk nsfkipqslg
      241 viggkpnlal yfigcvgnev iyldphttqr sgsvdkkvee deiemdatyh ckfasripit
      301 gmdpsvalcf fcatekdfkt lckiiqeeli tpekqpllel caerlahwsp aedvaaseai
      361 aapsfvdfeh idpqydtsde dfellg
//
LOCUS       XP_034196059             386 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 4a isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196059
VERSION     XP_034196059.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340168.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="autophagy-related 4a isoform X1"
                     /calculated_mol_wt=43298
     Region          38..323
                     /region_name="Peptidase_C54"
                     /note="Peptidase family C54; pfam03416"
                     /db_xref="CDD:460915"
     CDS             1..386
                     /gene="Atg4a"
                     /coded_by="XM_034340168.2:301..1461"
                     /db_xref="GeneID:117611823"
ORIGIN      
        1 mkmdslftqn isdepedipq tdepvwilgk kysaikelda irrdirsklw ftyrkgfvpi
       61 ggytstftsd kgwgcmlrcg qmvlghalii lhlgrdwqwt petrnstylk ilerfedrrt
      121 apfsihqias mgasegkevg qwfgpntiaq vlkklviyde wssitihval dntlivndil
      181 rqcrveggtt aeadgniplk apsqwkplll liplrlglse inpiyinglk nsfkipqslg
      241 viggkpnlal yfigcvgnev iyldphttqr sgsvdkkvee deiemdatyh ckfasripit
      301 gmdpsvalcf fcatekdfkt lckiiqeeli tpekqpllel caerlahwsp aedvaaseai
      361 aapsfvdfeh idpqydtsde dfellg
//
LOCUS       XP_034196060             386 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 4a isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196060
VERSION     XP_034196060.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340169.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="autophagy-related 4a isoform X1"
                     /calculated_mol_wt=43298
     Region          38..323
                     /region_name="Peptidase_C54"
                     /note="Peptidase family C54; pfam03416"
                     /db_xref="CDD:460915"
     CDS             1..386
                     /gene="Atg4a"
                     /coded_by="XM_034340169.2:215..1375"
                     /db_xref="GeneID:117611823"
ORIGIN      
        1 mkmdslftqn isdepedipq tdepvwilgk kysaikelda irrdirsklw ftyrkgfvpi
       61 ggytstftsd kgwgcmlrcg qmvlghalii lhlgrdwqwt petrnstylk ilerfedrrt
      121 apfsihqias mgasegkevg qwfgpntiaq vlkklviyde wssitihval dntlivndil
      181 rqcrveggtt aeadgniplk apsqwkplll liplrlglse inpiyinglk nsfkipqslg
      241 viggkpnlal yfigcvgnev iyldphttqr sgsvdkkvee deiemdatyh ckfasripit
      301 gmdpsvalcf fcatekdfkt lckiiqeeli tpekqpllel caerlahwsp aedvaaseai
      361 aapsfvdfeh idpqydtsde dfellg
//
LOCUS       XP_034196061             386 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 4a isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196061
VERSION     XP_034196061.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340170.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="autophagy-related 4a isoform X2"
                     /calculated_mol_wt=43376
     Region          38..323
                     /region_name="Peptidase_C54"
                     /note="Peptidase family C54; pfam03416"
                     /db_xref="CDD:460915"
     CDS             1..386
                     /gene="Atg4a"
                     /coded_by="XM_034340170.2:212..1372"
                     /db_xref="GeneID:117611823"
ORIGIN      
        1 mlffqvkggr yfdepedipq tdepvwilgk kysaikelda irrdirsklw ftyrkgfvpi
       61 ggytstftsd kgwgcmlrcg qmvlghalii lhlgrdwqwt petrnstylk ilerfedrrt
      121 apfsihqias mgasegkevg qwfgpntiaq vlkklviyde wssitihval dntlivndil
      181 rqcrveggtt aeadgniplk apsqwkplll liplrlglse inpiyinglk nsfkipqslg
      241 viggkpnlal yfigcvgnev iyldphttqr sgsvdkkvee deiemdatyh ckfasripit
      301 gmdpsvalcf fcatekdfkt lckiiqeeli tpekqpllel caerlahwsp aedvaaseai
      361 aapsfvdfeh idpqydtsde dfellg
//
LOCUS       XP_034196064             382 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 4a isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196064
VERSION     XP_034196064.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340173.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..382
                     /product="autophagy-related 4a isoform X3"
                     /calculated_mol_wt=42852
     Region          34..319
                     /region_name="Peptidase_C54"
                     /note="Peptidase family C54; pfam03416"
                     /db_xref="CDD:460915"
     CDS             1..382
                     /gene="Atg4a"
                     /coded_by="XM_034340173.2:212..1360"
                     /db_xref="GeneID:117611823"
ORIGIN      
        1 mlffqvkgde pedipqtdep vwilgkkysa ikeldairrd irsklwftyr kgfvpiggyt
       61 stftsdkgwg cmlrcgqmvl ghaliilhlg rdwqwtpetr nstylkiler fedrrtapfs
      121 ihqiasmgas egkevgqwfg pntiaqvlkk lviydewssi tihvaldntl ivndilrqcr
      181 veggttaead gniplkapsq wkpllllipl rlglseinpi yinglknsfk ipqslgvigg
      241 kpnlalyfig cvgneviyld phttqrsgsv dkkveedeie mdatyhckfa sripitgmdp
      301 svalcffcat ekdfktlcki iqeelitpek qpllelcaer lahwspaedv aaseaiaaps
      361 fvdfehidpq ydtsdedfel lg
//
LOCUS       XP_034196065             381 aa            linear   INV 03-SEP-2025
DEFINITION  autophagy-related 4a isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196065
VERSION     XP_034196065.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340174.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..381
                     /product="autophagy-related 4a isoform X4"
                     /calculated_mol_wt=42795
     Region          33..318
                     /region_name="Peptidase_C54"
                     /note="Peptidase family C54; pfam03416"
                     /db_xref="CDD:460915"
     CDS             1..381
                     /gene="Atg4a"
                     /coded_by="XM_034340174.2:212..1357"
                     /db_xref="GeneID:117611823"
ORIGIN      
        1 mlffqvkdep edipqtdepv wilgkkysai keldairrdi rsklwftyrk gfvpiggyts
       61 tftsdkgwgc mlrcgqmvlg haliilhlgr dwqwtpetrn stylkilerf edrrtapfsi
      121 hqiasmgase gkevgqwfgp ntiaqvlkkl viydewssit ihvaldntli vndilrqcrv
      181 eggttaeadg niplkapsqw kplllliplr lglseinpiy inglknsfki pqslgviggk
      241 pnlalyfigc vgneviyldp httqrsgsvd kkveedeiem datyhckfas ripitgmdps
      301 valcffcate kdfktlckii qeelitpekq pllelcaerl ahwspaedva aseaiaapsf
      361 vdfehidpqy dtsdedfell g
//
LOCUS       XP_034196066             204 aa            linear   INV 03-SEP-2025
DEFINITION  FGFR1 oncogene partner 2 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034196066
VERSION     XP_034196066.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340175.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..204
                     /product="FGFR1 oncogene partner 2 homolog"
                     /calculated_mol_wt=23314
     Region          2..180
                     /region_name="SIKE"
                     /note="SIKE family; pfam05769"
                     /db_xref="CDD:461735"
     CDS             1..204
                     /gene="LOC117611825"
                     /coded_by="XM_034340175.2:267..881"
                     /db_xref="GeneID:117611825"
ORIGIN      
        1 msltfqqiil daeklvtris dhentaddli ieiesvcgqi dnmkeykeev eilnaeakqk
       61 phlqlisgiq ienkhfqeiq aenkelknvl edyqhtleli mskyrqqtac llrlcktdip
      121 sthnakyani itsqaekine maaimktavs ldeenelkek emyyslkeen atlreivnia
      181 nkygslskdt evenktvqtd pvvl
//
LOCUS       XP_034196068             156 aa            linear   INV 03-SEP-2025
DEFINITION  U6 snRNA-associated Sm-like protein LSm1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196068
VERSION     XP_034196068.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340177.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196068.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..156
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..156
                     /product="U6 snRNA-associated Sm-like protein LSm1 isoform
                     X1"
                     /calculated_mol_wt=17828
     Region          26..99
                     /region_name="LSm1"
                     /note="Like-Sm protein 1; cd01728"
                     /db_xref="CDD:212475"
     Site            order(32,35..36,41,43..45,48,50,55..58,61..62,64,85..97)
                     /site_type="other"
                     /note="putative oligomer interface [polypeptide binding]"
                     /db_xref="CDD:212475"
     Site            order(40..46,48..60,62..66)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212475"
     Site            order(58,60,89)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212475"
     Site            85..96
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212475"
     CDS             1..156
                     /gene="LSm1"
                     /coded_by="XM_034340177.2:291..761"
                     /db_xref="GeneID:117611826"
ORIGIN      
        1 mchlgicesl ndtcsgyrky ckmnilpgta slleeldkkl mvllrdgrtl igylrsvdqf
       61 anivlhrtie rihvgkeygd iprgifivrg envvllgeid rdkekdlplt evsvddilda
      121 qrreqelkqd qkrlinkalk eralsyipdm ghddmf
//
LOCUS       XP_034196069             151 aa            linear   INV 03-SEP-2025
DEFINITION  U6 snRNA-associated Sm-like protein LSm1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196069
VERSION     XP_034196069.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340178.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196069.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="U6 snRNA-associated Sm-like protein LSm1 isoform
                     X2"
                     /calculated_mol_wt=17333
     Region          21..94
                     /region_name="LSm1"
                     /note="Like-Sm protein 1; cd01728"
                     /db_xref="CDD:212475"
     Site            order(27,30..31,36,38..40,43,45,50..53,56..57,59,80..92)
                     /site_type="other"
                     /note="putative oligomer interface [polypeptide binding]"
                     /db_xref="CDD:212475"
     Site            order(35..41,43..55,57..61)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212475"
     Site            order(53,55,84)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212475"
     Site            80..91
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212475"
     CDS             1..151
                     /gene="LSm1"
                     /coded_by="XM_034340178.2:237..692"
                     /db_xref="GeneID:117611826"
ORIGIN      
        1 mnaylndtcs gyrkyckmni lpgtasllee ldkklmvllr dgrtligylr svdqfanivl
       61 hrtierihvg keygdiprgi fivrgenvvl lgeidrdkek dlpltevsvd dildaqrreq
      121 elkqdqkrli nkalkerals yipdmghddm f
//
LOCUS       XP_034196070             134 aa            linear   INV 03-SEP-2025
DEFINITION  U6 snRNA-associated Sm-like protein LSm1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196070
VERSION     XP_034196070.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340179.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196070.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..134
                     /product="U6 snRNA-associated Sm-like protein LSm1 isoform
                     X3"
                     /calculated_mol_wt=15321
     Region          4..77
                     /region_name="LSm1"
                     /note="Like-Sm protein 1; cd01728"
                     /db_xref="CDD:212475"
     Site            order(10,13..14,19,21..23,26,28,33..36,39..40,42,63..75)
                     /site_type="other"
                     /note="putative oligomer interface [polypeptide binding]"
                     /db_xref="CDD:212475"
     Site            order(18..24,26..38,40..44)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212475"
     Site            order(36,38,67)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212475"
     Site            63..74
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212475"
     CDS             1..134
                     /gene="LSm1"
                     /coded_by="XM_034340179.2:266..670"
                     /db_xref="GeneID:117611826"
ORIGIN      
        1 mnilpgtasl leeldkklmv llrdgrtlig ylrsvdqfan ivlhrtieri hvgkeygdip
       61 rgifivrgen vvllgeidrd kekdlpltev svddildaqr reqelkqdqk rlinkalker
      121 alsyipdmgh ddmf
//
LOCUS       XP_034196071             134 aa            linear   INV 03-SEP-2025
DEFINITION  U6 snRNA-associated Sm-like protein LSm1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196071
VERSION     XP_034196071.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340180.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196071.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..134
                     /product="U6 snRNA-associated Sm-like protein LSm1 isoform
                     X3"
                     /calculated_mol_wt=15321
     Region          4..77
                     /region_name="LSm1"
                     /note="Like-Sm protein 1; cd01728"
                     /db_xref="CDD:212475"
     Site            order(10,13..14,19,21..23,26,28,33..36,39..40,42,63..75)
                     /site_type="other"
                     /note="putative oligomer interface [polypeptide binding]"
                     /db_xref="CDD:212475"
     Site            order(18..24,26..38,40..44)
                     /site_type="other"
                     /note="Sm1 motif"
                     /db_xref="CDD:212475"
     Site            order(36,38,67)
                     /site_type="other"
                     /note="putative RNA binding site [nucleotide binding]"
                     /db_xref="CDD:212475"
     Site            63..74
                     /site_type="other"
                     /note="Sm2 motif"
                     /db_xref="CDD:212475"
     CDS             1..134
                     /gene="LSm1"
                     /coded_by="XM_034340180.2:80..484"
                     /db_xref="GeneID:117611826"
ORIGIN      
        1 mnilpgtasl leeldkklmv llrdgrtlig ylrsvdqfan ivlhrtieri hvgkeygdip
       61 rgifivrgen vvllgeidrd kekdlpltev svddildaqr reqelkqdqk rlinkalker
      121 alsyipdmgh ddmf
//
LOCUS       XP_034196072              94 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome c oxidase assembly protein COX16 homolog l(3)neo43
            [Osmia lignaria lignaria].
ACCESSION   XP_034196072
VERSION     XP_034196072.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340181.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..94
                     /product="cytochrome c oxidase assembly protein COX16
                     homolog l(3)neo43"
                     /calculated_mol_wt=11115
     Region          14..88
                     /region_name="COX16"
                     /note="Cytochrome c oxidase assembly protein COX16;
                     pfam14138"
                     /db_xref="CDD:464091"
     CDS             1..94
                     /gene="l(3)neo43"
                     /coded_by="XM_034340181.2:230..514"
                     /db_xref="GeneID:117611827"
ORIGIN      
        1 mtqlyktkif kqfvpfmllv iggsflvref akirykyrkv ttydlakeae avgikmkkpa
       61 tleeeyentm kkididnwen irisrpweet tdtk
//
LOCUS       XP_034195852            2281 aa            linear   INV 03-SEP-2025
DEFINITION  unconventional myosin-IXb isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195852
VERSION     XP_034195852.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339961.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195852.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2281
                     /product="unconventional myosin-IXb isoform X1"
                     /calculated_mol_wt=259189
     Region          8..102
                     /region_name="RA_Myosin-IX"
                     /note="Ras-associating (RA) domain found in Myosin-IX;
                     cd01779"
                     /db_xref="CDD:340477"
     Region          148..992
                     /region_name="MYSc_Myo9"
                     /note="class IX myosin, motor domain; cd01385"
                     /db_xref="CDD:276836"
     Site            order(175..183,228..235,275..285,512..517)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276836"
     Site            175..183
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276836"
     Site            228..235
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276836"
     Site            275..285
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276836"
     Site            455
                     /site_type="other"
                     /note="putative phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:276836"
     Site            512..517
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276836"
     Site            order(542..553,556..565)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276836"
     Site            928..937
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276836"
     Site            order(940..967,971..992)
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276836"
     Region          1643..1696
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cd00029"
                     /db_xref="CDD:410341"
     Region          1878..1934
                     /region_name="C1_Myosin-IX"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in the unconventional myosin-IX family; cd20818"
                     /db_xref="CDD:410368"
     Region          1945..2131
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1982,2020,2024,2093,2096..2097,2122)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:239835"
     Site            1982
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239835"
     CDS             1..2281
                     /gene="LOC117611756"
                     /coded_by="XM_034339961.2:272..7117"
                     /db_xref="GeneID:117611756"
ORIGIN      
        1 mdncgsgvvq vfvgewspey ealsikatkq tssaeiveci ierlglvdps vgyelaevvg
       61 nsvgqecker rlgpsecpva lmllwpksaa qqeyyrfylr kkqsdylwsd srfpmdpqll
      121 kdyfnrflyq prdkeypdlc qlpdlneqtl ldnlrarfla gniytyvgsi lialnpfkfy
      181 piynpkyvkl yqnrrlgpdi pphifaiada ayhcmlkekr nqcivisges gsgktestnf
      241 llhhltalsq kgshgsgveq tilsagpvle afgnaktahn nnssrfgkfi qvnykengmv
      301 hgavvqkyll eksrivsqgr nernyhvfyy llagaseqek qllhlescdr ynylnksgcy
      361 gleniderhe fsrlkqsmem vgftpekqrr lfavlsavll lgnvefqprk syhhhdeavg
      421 vknpevvali sellrvkqet llaaltakra rasgetlvin yrlpeaiaar damakclyga
      481 lfdwivlqvn hallskkdtl rdhqgnsigv ldifgfedfk tcnsfeqlci nyaneqlqhy
      541 fnqhvfqyeq reyrkqgirw tdigysdnsg clnliegkpn gllcllddqc nfpgatnetl
      601 lqkfnsvhkd npfyeapqrr eaafvvrhya gavkyqasnm reknldlmrp dgvvgvlkns
      661 slafvrelvg adpvavfrwa ilraffrahf afqeagrahr hgradgtkas vqnryrtpne
      721 nlisshkhnr ppsyqkqrsv cipsaipttt lssiqletnd nvnnnrlswp qfrninqtqh
      781 psnvttmkgy lirgredqph snnklrrdth tplervlpkd eanvmeranq ivmknksfrp
      841 rergkkglkn lqtvktlagr iqsygtgpgk arkqpmtvsa qfqqslhslm dtlnqanpff
      901 irciksnank vpnefdeetv qrqlrytgml etvrirqagf nvrltyeefi qlyrmllpkg
      961 llssqsdvrd flltlnlnrd nyqlgttkvf lresekikld ielhqqiits ittiqkwfra
     1021 clerrkflrl rnavvqiqsf wrmviaqrfa hslrsrieaa thiqsawray kqqswfkklk
     1081 scvitfqahv rgnnarkafa elkkqkkllv gkieeykesq dqrelaydns kiysrlrdsd
     1141 esltedhgll eyntlrksef qnrlpstrad nilrdsnidt smsyskrkvt pttrilqess
     1201 ailrnteplf tsedfnerks slesltslrs yesqvsvnss esqensyckp vpstrtkrgd
     1261 qspvvatntg lmnassrlss vskradssst gysdgetdte ipsavhsapp vfnatppfps
     1321 preikyhqsn vnlshspnsd vwrrradlss inysdyllsg pqkttltrsp nvscyknitd
     1381 nmfdqtqqek pheasnynvk ldpnerfvhm esssgreqrr lsdnnvpser ienvpassar
     1441 rdqtyvhfny lrrqnsegdt smkldavnde nvlkssllkg ttskeknsrp kekcepdvpv
     1501 rsarrnrpsr evqcrsmges vseagsgetk slflgtikes dlhkstnvrt rkdsphevas
     1561 rsvtdwpvnk nesgsktqqp gkrmrsnait tlelrrrnsd patkisglse dkagtdtspn
     1621 dlklapgmnl lewkgnlftl aghcfrkvar fakddvcvcc hekmdafvtq gykcidckql
     1681 yhvkciqngg vlkmpcilan tpgrrknrkp prtpydaskq tvapkfsltg tsafsdstdk
     1741 iisdakelal mqdfitkkiy imegqeegrk psevdrvfkq alrkfkddlv itysvaiqqr
     1801 vegnikytdl ianflhvmet vckqentted fpvtmgvnaf rgfmnefmtl vkteapekqs
     1861 kskrkkekkr kqeeptrhgn hmfqltiini ptacevctsf fmwpierglv cqnckltchk
     1921 kcymrasaec gkdaslhemn shkvfgvply kldcgdgkvp vvvdrlitti emhglytegi
     1981 yrksgvsskv kelklkmdeg dlekvdfeny qvhvlaavlk sffrdmpepl ltfeyyddfl
     2041 haanltdprd ristlfailk klpkpnfdlm erlivhlarv alhevdnrms psalaivfap
     2101 cilrtnralp aqdslqdvgr qtccvetivq eklrvvratl adintlesac htathrlssl
     2161 rsskifspee lnaatpnata rcgptdrdrd rgdeeeailv dhiqeiqkek alltstlpsl
     2221 trassdddll lsatdlddgs lddllpssad glvrkkpiqr qssadnafpt iisvdedmvm
     2281 v
//
LOCUS       XP_034195853            2281 aa            linear   INV 03-SEP-2025
DEFINITION  unconventional myosin-IXb isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195853
VERSION     XP_034195853.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339962.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195853.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2281
                     /product="unconventional myosin-IXb isoform X1"
                     /calculated_mol_wt=259189
     Region          8..102
                     /region_name="RA_Myosin-IX"
                     /note="Ras-associating (RA) domain found in Myosin-IX;
                     cd01779"
                     /db_xref="CDD:340477"
     Region          148..992
                     /region_name="MYSc_Myo9"
                     /note="class IX myosin, motor domain; cd01385"
                     /db_xref="CDD:276836"
     Site            order(175..183,228..235,275..285,512..517)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276836"
     Site            175..183
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276836"
     Site            228..235
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276836"
     Site            275..285
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276836"
     Site            455
                     /site_type="other"
                     /note="putative phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:276836"
     Site            512..517
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276836"
     Site            order(542..553,556..565)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276836"
     Site            928..937
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276836"
     Site            order(940..967,971..992)
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276836"
     Region          1643..1696
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cd00029"
                     /db_xref="CDD:410341"
     Region          1878..1934
                     /region_name="C1_Myosin-IX"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in the unconventional myosin-IX family; cd20818"
                     /db_xref="CDD:410368"
     Region          1945..2131
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1982,2020,2024,2093,2096..2097,2122)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:239835"
     Site            1982
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239835"
     CDS             1..2281
                     /gene="LOC117611756"
                     /coded_by="XM_034339962.2:957..7802"
                     /db_xref="GeneID:117611756"
ORIGIN      
        1 mdncgsgvvq vfvgewspey ealsikatkq tssaeiveci ierlglvdps vgyelaevvg
       61 nsvgqecker rlgpsecpva lmllwpksaa qqeyyrfylr kkqsdylwsd srfpmdpqll
      121 kdyfnrflyq prdkeypdlc qlpdlneqtl ldnlrarfla gniytyvgsi lialnpfkfy
      181 piynpkyvkl yqnrrlgpdi pphifaiada ayhcmlkekr nqcivisges gsgktestnf
      241 llhhltalsq kgshgsgveq tilsagpvle afgnaktahn nnssrfgkfi qvnykengmv
      301 hgavvqkyll eksrivsqgr nernyhvfyy llagaseqek qllhlescdr ynylnksgcy
      361 gleniderhe fsrlkqsmem vgftpekqrr lfavlsavll lgnvefqprk syhhhdeavg
      421 vknpevvali sellrvkqet llaaltakra rasgetlvin yrlpeaiaar damakclyga
      481 lfdwivlqvn hallskkdtl rdhqgnsigv ldifgfedfk tcnsfeqlci nyaneqlqhy
      541 fnqhvfqyeq reyrkqgirw tdigysdnsg clnliegkpn gllcllddqc nfpgatnetl
      601 lqkfnsvhkd npfyeapqrr eaafvvrhya gavkyqasnm reknldlmrp dgvvgvlkns
      661 slafvrelvg adpvavfrwa ilraffrahf afqeagrahr hgradgtkas vqnryrtpne
      721 nlisshkhnr ppsyqkqrsv cipsaipttt lssiqletnd nvnnnrlswp qfrninqtqh
      781 psnvttmkgy lirgredqph snnklrrdth tplervlpkd eanvmeranq ivmknksfrp
      841 rergkkglkn lqtvktlagr iqsygtgpgk arkqpmtvsa qfqqslhslm dtlnqanpff
      901 irciksnank vpnefdeetv qrqlrytgml etvrirqagf nvrltyeefi qlyrmllpkg
      961 llssqsdvrd flltlnlnrd nyqlgttkvf lresekikld ielhqqiits ittiqkwfra
     1021 clerrkflrl rnavvqiqsf wrmviaqrfa hslrsrieaa thiqsawray kqqswfkklk
     1081 scvitfqahv rgnnarkafa elkkqkkllv gkieeykesq dqrelaydns kiysrlrdsd
     1141 esltedhgll eyntlrksef qnrlpstrad nilrdsnidt smsyskrkvt pttrilqess
     1201 ailrnteplf tsedfnerks slesltslrs yesqvsvnss esqensyckp vpstrtkrgd
     1261 qspvvatntg lmnassrlss vskradssst gysdgetdte ipsavhsapp vfnatppfps
     1321 preikyhqsn vnlshspnsd vwrrradlss inysdyllsg pqkttltrsp nvscyknitd
     1381 nmfdqtqqek pheasnynvk ldpnerfvhm esssgreqrr lsdnnvpser ienvpassar
     1441 rdqtyvhfny lrrqnsegdt smkldavnde nvlkssllkg ttskeknsrp kekcepdvpv
     1501 rsarrnrpsr evqcrsmges vseagsgetk slflgtikes dlhkstnvrt rkdsphevas
     1561 rsvtdwpvnk nesgsktqqp gkrmrsnait tlelrrrnsd patkisglse dkagtdtspn
     1621 dlklapgmnl lewkgnlftl aghcfrkvar fakddvcvcc hekmdafvtq gykcidckql
     1681 yhvkciqngg vlkmpcilan tpgrrknrkp prtpydaskq tvapkfsltg tsafsdstdk
     1741 iisdakelal mqdfitkkiy imegqeegrk psevdrvfkq alrkfkddlv itysvaiqqr
     1801 vegnikytdl ianflhvmet vckqentted fpvtmgvnaf rgfmnefmtl vkteapekqs
     1861 kskrkkekkr kqeeptrhgn hmfqltiini ptacevctsf fmwpierglv cqnckltchk
     1921 kcymrasaec gkdaslhemn shkvfgvply kldcgdgkvp vvvdrlitti emhglytegi
     1981 yrksgvsskv kelklkmdeg dlekvdfeny qvhvlaavlk sffrdmpepl ltfeyyddfl
     2041 haanltdprd ristlfailk klpkpnfdlm erlivhlarv alhevdnrms psalaivfap
     2101 cilrtnralp aqdslqdvgr qtccvetivq eklrvvratl adintlesac htathrlssl
     2161 rsskifspee lnaatpnata rcgptdrdrd rgdeeeailv dhiqeiqkek alltstlpsl
     2221 trassdddll lsatdlddgs lddllpssad glvrkkpiqr qssadnafpt iisvdedmvm
     2281 v
//
LOCUS       XP_076546715            2172 aa            linear   INV 03-SEP-2025
DEFINITION  unconventional myosin-IXa isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546715
VERSION     XP_076546715.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690600.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2172
                     /product="unconventional myosin-IXa isoform X2"
                     /calculated_mol_wt=246637
     Region          8..102
                     /region_name="RA_Myosin-IX"
                     /note="Ras-associating (RA) domain found in Myosin-IX;
                     cd01779"
                     /db_xref="CDD:340477"
     Region          148..883
                     /region_name="MYSc_Myo9"
                     /note="class IX myosin, motor domain; cd01385"
                     /db_xref="CDD:276836"
     Site            order(175..183,228..235,275..285,512..517)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276836"
     Site            175..183
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276836"
     Site            228..235
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276836"
     Site            275..285
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276836"
     Site            455
                     /site_type="other"
                     /note="putative phosphorylation site [posttranslational
                     modification]"
                     /db_xref="CDD:276836"
     Site            512..517
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276836"
     Site            order(542..553,556..565)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276836"
     Site            819..828
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276836"
     Site            order(831..858,862..883)
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276836"
     Region          1534..1587
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cd00029"
                     /db_xref="CDD:410341"
     Region          1769..1825
                     /region_name="C1_Myosin-IX"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     found in the unconventional myosin-IX family; cd20818"
                     /db_xref="CDD:410368"
     Region          1836..2022
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     Site            order(1873,1911,1915,1984,1987..1988,2013)
                     /site_type="other"
                     /note="GTPase interaction site"
                     /db_xref="CDD:239835"
     Site            1873
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239835"
     CDS             1..2172
                     /gene="LOC117611756"
                     /coded_by="XM_076690600.1:268..6786"
                     /db_xref="GeneID:117611756"
ORIGIN      
        1 mdncgsgvvq vfvgewspey ealsikatkq tssaeiveci ierlglvdps vgyelaevvg
       61 nsvgqecker rlgpsecpva lmllwpksaa qqeyyrfylr kkqsdylwsd srfpmdpqll
      121 kdyfnrflyq prdkeypdlc qlpdlneqtl ldnlrarfla gniytyvgsi lialnpfkfy
      181 piynpkyvkl yqnrrlgpdi pphifaiada ayhcmlkekr nqcivisges gsgktestnf
      241 llhhltalsq kgshgsgveq tilsagpvle afgnaktahn nnssrfgkfi qvnykengmv
      301 hgavvqkyll eksrivsqgr nernyhvfyy llagaseqek qllhlescdr ynylnksgcy
      361 gleniderhe fsrlkqsmem vgftpekqrr lfavlsavll lgnvefqprk syhhhdeavg
      421 vknpevvali sellrvkqet llaaltakra rasgetlvin yrlpeaiaar damakclyga
      481 lfdwivlqvn hallskkdtl rdhqgnsigv ldifgfedfk tcnsfeqlci nyaneqlqhy
      541 fnqhvfqyeq reyrkqgirw tdigysdnsg clnliegkpn gllcllddqc nfpgatnetl
      601 lqkfnsvhkd npfyeapqrr eaafvvrhya gavkyqasnm reknldlmrp dgvvgvlkns
      661 slafvrelvg adpvavfrwa ilraffrahf afqeagrahr hgradgtkas vqnryrtpne
      721 nlirknksfr prergkkglk nlqtvktlag riqsygtgpg karkqpmtvs aqfqqslhsl
      781 mdtlnqanpf firciksnan kvpnefdeet vqrqlrytgm letvrirqag fnvrltyeef
      841 iqlyrmllpk gllssqsdvr dflltlnlnr dnyqlgttkv flresekikl dielhqqiit
      901 sittiqkwfr aclerrkflr lrnavvqiqs fwrmviaqrf ahslrsriea athiqsawra
      961 ykqqswfkkl kscvitfqah vrgnnarkaf aelkkqkkll vgkieeykes qdqrelaydn
     1021 skiysrlrds desltedhgl leyntlrkse fqnrlpstra dnilrdsnid tsmsyskrkv
     1081 tpttrilqes sailrntepl ftsedfnerk sslesltslr syesqvsvns sesqensyck
     1141 pvpstrtkrg dqspvvatnt glmnassrls svskradsss tgysdgetdt eipsavhsap
     1201 pvfnatppfp spreikyhqs nvnlshspns dvwrrradls sinysdylls gpqkttltrs
     1261 pnvscyknit dnmfdqtqqe kpheasnynv kldpnerfvh messsgreqr rlsdnnvpse
     1321 rienvpassa rrdqtyvhfn ylrrqnsegd tsmkldavnd envlkssllk gttskeknsr
     1381 pkekcepdvp vrsarrnrps revqcrsmge svseagsget kslflgtike sdlhkstnvr
     1441 trkdspheva srsvtdwpvn knesgsktqq pgkrmrsnai ttlelrrrns dpatkisgls
     1501 edkagtdtsp ndlklapgmn llewkgnlft laghcfrkva rfakddvcvc chekmdafvt
     1561 qgykcidckq lyhvkciqng gvlkmpcila ntpgrrknrk pprtpydask qtvapkfslt
     1621 gtsafsdstd kiisdakela lmqdfitkki yimegqeegr kpsevdrvfk qalrkfkddl
     1681 vitysvaiqq rvegnikytd lianflhvme tvckqentte dfpvtmgvna frgfmnefmt
     1741 lvkteapekq skskrkkekk rkqeeptrhg nhmfqltiin iptacevcts ffmwpiergl
     1801 vcqnckltch kkcymrasae cgkdaslhem nshkvfgvpl ykldcgdgkv pvvvdrlitt
     1861 iemhglyteg iyrksgvssk vkelklkmde gdlekvdfen yqvhvlaavl ksffrdmpep
     1921 lltfeyyddf lhaanltdpr dristlfail kklpkpnfdl merlivhlar valhevdnrm
     1981 spsalaivfa pcilrtnral paqdslqdvg rqtccvetiv qeklrvvrat ladintlesa
     2041 chtathrlss lrsskifspe elnaatpnat arcgptdrdr drgdeeeail vdhiqeiqke
     2101 kalltstlps ltrassdddl llsatdlddg slddllpssa dglvrkkpiq rqssadnafp
     2161 tiisvdedmv mv
//
LOCUS       XP_034195855             330 aa            linear   INV 03-SEP-2025
DEFINITION  3-ketodihydrosphingosine reductase [Osmia lignaria lignaria].
ACCESSION   XP_034195855
VERSION     XP_034195855.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339964.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="3-ketodihydrosphingosine reductase"
                     /calculated_mol_wt=36158
     Region          33..267
                     /region_name="KDSR-like_SDR_c"
                     /note="3-ketodihydrosphingosine reductase (KDSR) and
                     related proteins, classical (c) SDR; cd08939"
                     /db_xref="CDD:187643"
     Site            order(39,41..44,63..65,93..95,122..124,145,172..174,187,
                     191,217..220,222..223)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187643"
     Site            order(99,130..132,134,139,143,151,155,158..159,180..183,
                     185,188..189,192..193,195..197,199..201,203..204,211,250,
                     254,258,260..261,267)
                     /site_type="other"
                     /note="homotetramer interface [polypeptide binding]"
                     /db_xref="CDD:187643"
     Site            order(146,174,187,191)
                     /site_type="active"
                     /db_xref="CDD:187643"
     CDS             1..330
                     /gene="Kdsr"
                     /coded_by="XM_034339964.2:530..1522"
                     /db_xref="GeneID:117611757"
ORIGIN      
        1 mlkialiiis lviaalfary yfwrgrlknv nnkhvvvtgg ssgigkcvai laakygahvt
       61 iiardvqkle aarneiirac knknvqkvey lsldigddyn mvekalndle rtmgpiymlv
      121 ncagtaicgk iedtsvedln kmihinflgs yyctkavvpr mkasqdgiiv ltssqaallg
      181 ifgysaycst kfalrglaes ismelapyni svtlslppdt dtpgfaieel skpletklis
      241 qtsslvspev vadklfkdal agkffstvgi egfmltvlcv gmspfnsime llsqsmlvgl
      301 frlvsawylv nfkkiiqncm kmrdknkkse
//
LOCUS       XP_034195857             304 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195857
VERSION     XP_034195857.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339966.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="neurotrimin isoform X1"
                     /calculated_mol_wt=34333
     Region          50..143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          61..65
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          74..79
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          110..114
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..129
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          136..139
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          154..241
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          164..168
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          180..183
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          210..214
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          224..229
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          234..237
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..304
                     /gene="LOC117611758"
                     /coded_by="XM_034339966.2:2945..3859"
                     /db_xref="GeneID:117611758"
ORIGIN      
        1 mrrscyvnyy vlclvcwill gitgvstrsh slvkrfdgmy mgpyfdsnip tnitaqlgsh
       61 aylpckvrql gnksvswvrr rdshilsvdr nmfipderfq alfvdasdtw tlqvkyvqar
      121 degeyecqis tdpkkshiik lnvvvpkiei igdrdmyvkt gstvaircvi kqslegpfyv
      181 fwyhegdrvl dyqlnkidiq tekidhdtvs slvihkakre dsgnytcsps sldsasvqlh
      241 vlngehpaai qrgissapgg cptlwwlagv vtlysshgsr lfvlallilm vlysknpsyf
      301 aper
//
LOCUS       XP_034195858             292 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195858
VERSION     XP_034195858.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339967.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195858.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="neurotrimin isoform X2"
                     /calculated_mol_wt=32975
     Region          38..131
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          49..53
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          62..67
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          98..102
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          112..117
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..127
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          142..229
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          152..156
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          167..171
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          198..202
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..292
                     /gene="LOC117611758"
                     /coded_by="XM_034339967.2:203..1081"
                     /db_xref="GeneID:117611758"
ORIGIN      
        1 mvrhtikwii kgvstrshsl vkrfdgmymg pyfdsniptn itaqlgshay lpckvrqlgn
       61 ksvswvrrrd shilsvdrnm fipderfqal fvdasdtwtl qvkyvqarde geyecqistd
      121 pkkshiikln vvvpkieiig drdmyvktgs tvaircvikq slegpfyvfw yhegdrvldy
      181 qlnkidiqte kidhdtvssl vihkakreds gnytcspssl dsasvqlhvl ngehpaaiqr
      241 gissapggcp tlwwlagvvt lysshgsrlf vlallilmvl ysknpsyfap er
//
LOCUS       XP_076546716             284 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546716
VERSION     XP_076546716.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690601.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="neurotrimin isoform X3"
                     /calculated_mol_wt=31899
     Region          30..123
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          41..45
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          54..59
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          90..94
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          104..109
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          116..119
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          134..221
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          144..148
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          159..163
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          190..194
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..284
                     /gene="LOC117611758"
                     /coded_by="XM_076690601.1:8362..9216"
                     /db_xref="GeneID:117611758"
ORIGIN      
        1 makgvstrsh slvkrfdgmy mgpyfdsnip tnitaqlgsh aylpckvrql gnksvswvrr
       61 rdshilsvdr nmfipderfq alfvdasdtw tlqvkyvqar degeyecqis tdpkkshiik
      121 lnvvvpkiei igdrdmyvkt gstvaircvi kqslegpfyv fwyhegdrvl dyqlnkidiq
      181 tekidhdtvs slvihkakre dsgnytcsps sldsasvqlh vlngehpaai qrgissapgg
      241 cptlwwlagv vtlysshgsr lfvlallilm vlysknpsyf aper
//
LOCUS       XP_034195862             269 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195862
VERSION     XP_034195862.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339971.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="neurotrimin isoform X4"
                     /calculated_mol_wt=30715
     Region          50..143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          61..65
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          74..79
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          110..114
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..129
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          136..139
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          154..241
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          164..168
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          180..183
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          210..214
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          224..229
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          234..237
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..269
                     /gene="LOC117611758"
                     /coded_by="XM_034339971.2:5926..6735"
                     /db_xref="GeneID:117611758"
ORIGIN      
        1 mrrscyvnyy vlclvcwill gitgvstrsh slvkrfdgmy mgpyfdsnip tnitaqlgsh
       61 aylpckvrql gnksvswvrr rdshilsvdr nmfipderfq alfvdasdtw tlqvkyvqar
      121 degeyecqis tdpkkshiik lnvvvpkiei igdrdmyvkt gstvaircvi kqslegpfyv
      181 fwyhegdrvl dyqlnkidiq tekidhdtvs slvihkakre dsgnytcsps sldsasvqlh
      241 vlndldqaiq sklnhnvhsv slkktlkeh
//
LOCUS       XP_034195863             268 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034195863
VERSION     XP_034195863.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339972.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="neurotrimin isoform X5"
                     /calculated_mol_wt=30539
     Region          50..143
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          61..65
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          74..79
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          110..114
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..129
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          136..139
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          154..241
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          164..168
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          180..183
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          210..214
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          224..229
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          234..237
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..268
                     /gene="LOC117611758"
                     /coded_by="XM_034339972.2:5926..6732"
                     /db_xref="GeneID:117611758"
ORIGIN      
        1 mrrscyvnyy vlclvcwill gitgvstrsh slvkrfdgmy mgpyfdsnip tnitaqlgsh
       61 aylpckvrql gnksvswvrr rdshilsvdr nmfipderfq alfvdasdtw tlqvkyvqar
      121 degeyecqis tdpkkshiik lnvvvpkiei igdrdmyvkt gstvaircvi kqslegpfyv
      181 fwyhegdrvl dyqlnkidiq tekidhdtvs slvihkakre dsgnytcsps sldsasvqlh
      241 vlngqfsrlr ragivsntda kegetree
//
LOCUS       XP_034195864             266 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034195864
VERSION     XP_034195864.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339973.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..266
                     /product="neurotrimin isoform X6"
                     /calculated_mol_wt=29940
     Region          12..105
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          23..27
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          36..41
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          72..76
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          86..91
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          98..101
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          116..203
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          126..130
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          141..145
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          172..176
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..266
                     /gene="LOC117611758"
                     /coded_by="XM_034339973.2:2303..3103"
                     /db_xref="GeneID:117611758"
ORIGIN      
        1 mymgpyfdsn iptnitaqlg shaylpckvr qlgnksvswv rrrdshilsv drnmfipder
       61 fqalfvdasd twtlqvkyvq ardegeyecq istdpkkshi iklnvvvpki eiigdrdmyv
      121 ktgstvairc vikqslegpf yvfwyhegdr vldyqlnkid iqtekidhdt vsslvihkak
      181 redsgnytcs pssldsasvq lhvlngehpa aiqrgissap ggcptlwwla gvvtlysshg
      241 srlfvlalli lmvlysknps yfaper
//
LOCUS       XP_034195592            1268 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611662 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195592
VERSION     XP_034195592.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339701.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195592.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1268
                     /product="uncharacterized protein LOC117611662 isoform X1"
                     /calculated_mol_wt=142936
     Region          <614..971
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1268
                     /gene="LOC117611662"
                     /coded_by="XM_034339701.2:944..4750"
                     /db_xref="GeneID:117611662"
ORIGIN      
        1 minipynvpl ispimptqnv pvqtshnhfq pfqqpqtntq qmqtnpafpm nmqdpykapf
       61 yqfqqsdmfm anqyvpnvly prtfqpanni qpplhtqpny apllqnlleq irgqvsntap
      121 ifilpggcqq stnhhqqagm pnqgggpqat gqptsfnyps svhcppsfyp ypiplpvyqp
      181 fvgqtrnges srgcrcchrr qessdlksys lncccnlaeq edhrctatnd dtvcskrncp
      241 asvslqalas qflslpgivs caatrlvlrk vpgsnitstm edtmdkaqka ismltkeqll
      301 tesrnaqqvn alinlhmttn ppaniipllt liqlkvnvlk aqieslinkk vmecqgfgye
      361 vetsgpidpt vlsmktneel rhllsalrqk ecdervnvnf spyhsqrvia earlnnvqak
      421 irqveaefdr rrsvslplps ltsrviqqfa estctfgfaq tklfepypqe nplqspdpfs
      481 asirnpkrly lkpraaesea trqkdqskat stsqdappsc kdtgtgegna dcskersvgp
      541 rssedscsch ltsseesidq gkkklrlrid ragkvtissa gkledaslan fspnvvvstd
      601 qyrctfngrc vsikeddiad repeireife vedertedpk tdadtlelfd lprvkkeped
      661 atdvetsenv dvpikikepe dephykatml idlkskktem etqpsaaate kriqikeqpk
      721 ilkkikvkdi kvedktetqd vktehmdlkp ekkrevhvds kpkkvktely inlegpspet
      781 kdikaheklt aevpeekvtt tppseqtiek kpssdipksf fgitlwkage skkkpkttah
      841 edehskdkik seddeasaad mkekliveda skqqatesam ladeqltdke ssevkknyfg
      901 itlkkrgepk rrpktendks hpkdgehenv qiismvthme ydrgfgdfdg wvdtkdyidr
      961 kikdkertkd nclmsikher mfpknhqpsd ekhingiarf pplknaaqtv tnipenifhq
     1021 eeiesgssra vynrrvtyhp dsgnsivsti ysvienqlts ftslfanipn lqnivsslcy
     1081 dklpseqnvs tlnpetivnr kesnkeisge ddwtllpgdd dgtsdescvs inegfsnihk
     1141 ikqsiivdke nknyvliegs fsgqlnelsr nglqrpyhtr rtlvirsrlk npgrlkdqrp
     1201 ftilkrskkn kvlnhqetkq vkhqsktskd fcfknntkin kncsfpsvdr slsknkkifs
     1261 dsgvnkqv
//
LOCUS       XP_076546717            1246 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611662 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546717
VERSION     XP_076546717.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690602.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1246
                     /product="uncharacterized protein LOC117611662 isoform X2"
                     /calculated_mol_wt=140604
     Region          <5..159
                     /region_name="SOBP"
                     /note="Sine oculis-binding protein; pfam15279"
                     /db_xref="CDD:464609"
     Region          <592..949
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1246
                     /gene="LOC117611662"
                     /coded_by="XM_076690602.1:944..4684"
                     /db_xref="GeneID:117611662"
ORIGIN      
        1 minipynvpl ispimptqnv pvqtshnhfq pfqqpqtntq qmqtnpafpm nmqdpykapf
       61 yqfqqsdmfm anqyvpnvly prtfqpanni qpplhtqpny apllqnlleq irgqqagmpn
      121 qgggpqatgq ptsfnypssv hcppsfypyp iplpvyqpfv gqtrngessr gcrcchrrqe
      181 ssdlksysln cccnlaeqed hrctatnddt vcskrncpas vslqalasqf lslpgivsca
      241 atrlvlrkvp gsnitstmed tmdkaqkais mltkeqllte srnaqqvnal inlhmttnpp
      301 aniiplltli qlkvnvlkaq ieslinkkvm ecqgfgyeve tsgpidptvl smktneelrh
      361 llsalrqkec dervnvnfsp yhsqrviaea rlnnvqakir qveaefdrrr svslplpslt
      421 srviqqfaes tctfgfaqtk lfepypqenp lqspdpfsas irnpkrlylk praaeseatr
      481 qkdqskatst sqdappsckd tgtgegnadc skersvgprs sedscschlt sseesidqgk
      541 kklrlridra gkvtissagk ledaslanfs pnvvvstdqy rctfngrcvs ikeddiadre
      601 peireifeve dertedpktd adtlelfdlp rvkkepedat dvetsenvdv pikikepede
      661 phykatmlid lkskktemet qpsaaatekr iqikeqpkil kkikvkdikv edktetqdvk
      721 tehmdlkpek krevhvdskp kkvktelyin legpspetkd ikahekltae vpeekvtttp
      781 pseqtiekkp ssdipksffg itlwkagesk kkpkttahed ehskdkikse ddeasaadmk
      841 eklivedask qqatesamla deqltdkess evkknyfgit lkkrgepkrr pktendkshp
      901 kdgehenvqi ismvthmeyd rgfgdfdgwv dtkdyidrki kdkertkdnc lmsikhermf
      961 pknhqpsdek hingiarfpp lknaaqtvtn ipenifhqee iesgssravy nrrvtyhpds
     1021 gnsivstiys vienqltsft slfanipnlq nivsslcydk lpseqnvstl npetivnrke
     1081 snkeisgedd wtllpgdddg tsdescvsin egfsnihkik qsiivdkenk nyvliegsfs
     1141 gqlnelsrng lqrpyhtrrt lvirsrlknp grlkdqrpft ilkrskknkv lnhqetkqvk
     1201 hqsktskdfc fknntkinkn csfpsvdrsl sknkkifsds gvnkqv
//
LOCUS       XP_034195593            1223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611662 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195593
VERSION     XP_034195593.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339702.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195593.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1223
                     /product="uncharacterized protein LOC117611662 isoform X3"
                     /calculated_mol_wt=137713
     Region          <569..926
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1223
                     /gene="LOC117611662"
                     /coded_by="XM_034339702.2:120..3791"
                     /db_xref="GeneID:117611662"
ORIGIN      
        1 mksigllhdp ykapfyqfqq sdmfmanqyv pnvlyprtfq panniqpplh tqpnyapllq
       61 nlleqirgqv sntapifilp ggcqqstnhh qqagmpnqgg gpqatgqpts fnypssvhcp
      121 psfypypipl pvyqpfvgqt rngessrgcr cchrrqessd lksyslnccc nlaeqedhrc
      181 tatnddtvcs krncpasvsl qalasqflsl pgivscaatr lvlrkvpgsn itstmedtmd
      241 kaqkaismlt keqlltesrn aqqvnalinl hmttnppani iplltliqlk vnvlkaqies
      301 linkkvmecq gfgyevetsg pidptvlsmk tneelrhlls alrqkecder vnvnfspyhs
      361 qrviaearln nvqakirqve aefdrrrsvs lplpsltsrv iqqfaestct fgfaqtklfe
      421 pypqenplqs pdpfsasirn pkrlylkpra aeseatrqkd qskatstsqd appsckdtgt
      481 gegnadcske rsvgprssed scschltsse esidqgkkkl rlridragkv tissagkled
      541 aslanfspnv vvstdqyrct fngrcvsike ddiadrepei reifeveder tedpktdadt
      601 lelfdlprvk kepedatdve tsenvdvpik ikepedephy katmlidlks kktemetqps
      661 aaatekriqi keqpkilkki kvkdikvedk tetqdvkteh mdlkpekkre vhvdskpkkv
      721 ktelyinleg pspetkdika hekltaevpe ekvtttppse qtiekkpssd ipksffgitl
      781 wkageskkkp kttahedehs kdkiksedde asaadmkekl ivedaskqqa tesamladeq
      841 ltdkessevk knyfgitlkk rgepkrrpkt endkshpkdg ehenvqiism vthmeydrgf
      901 gdfdgwvdtk dyidrkikdk ertkdnclms ikhermfpkn hqpsdekhin giarfpplkn
      961 aaqtvtnipe nifhqeeies gssravynrr vtyhpdsgns ivstiysvie nqltsftslf
     1021 anipnlqniv sslcydklps eqnvstlnpe tivnrkesnk eisgeddwtl lpgdddgtsd
     1081 escvsinegf snihkikqsi ivdkenknyv liegsfsgql nelsrnglqr pyhtrrtlvi
     1141 rsrlknpgrl kdqrpftilk rskknkvlnh qetkqvkhqs ktskdfcfkn ntkinkncsf
     1201 psvdrslskn kkifsdsgvn kqv
//
LOCUS       XP_034195597             582 aa            linear   INV 03-SEP-2025
DEFINITION  UFM1 specific peptidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034195597
VERSION     XP_034195597.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339706.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..582
                     /product="UFM1 specific peptidase 2"
                     /calculated_mol_wt=65694
     Region          390..574
                     /region_name="Peptidase_C78"
                     /note="Peptidase family C78; pfam07910"
                     /db_xref="CDD:400318"
     CDS             1..582
                     /gene="Ufsp2"
                     /coded_by="XM_034339706.2:231..1979"
                     /db_xref="GeneID:117611663"
ORIGIN      
        1 mapqlqilsn vierlksind gvtghlygvm ynntltvltf sinvlnntev ninhttlqlh
       61 mpaevylcgi lhvgqceekm pdsfqdidit dnplflkyth dslkveayfy ihqkletvdd
      121 vnvinendiy qeftyirlkg slplivqngn vtevleeirr niasgkigiq fpsnntflsn
      181 ndngikdtsl kelldmhehy egsknikrgi mrstgvvdav naniflkisg drlteenikc
      241 apvlqyikrp fnslecnllv dtlslanlnm spadlygvlv esmcrnikli ekcfedqlqn
      301 seviklpvpl hfkaqgfghl ltvvypkgyt dketmkyred lhiilglemd kpyfrygnal
      361 kfydsqaeni linpheaiqq nhdaasnsrk vsivqglyay hhymqdnfdd ngwgcayrsl
      421 qtivswyrlq gytdipipsh stiqkclvni gdkpsnfvns kqwigstevg fvlesllgis
      481 vkilcastge evsalavnll hhfqtqstpv migggvlaht ilgvsydeis gdvkflildp
      541 hytgsehlpt iinkgwcgwk tkdfwkndaf ynmclpqrpv ci
//
LOCUS       XP_034195598             501 aa            linear   INV 03-SEP-2025
DEFINITION  lysophospholipid acyltransferase 6 [Osmia lignaria lignaria].
ACCESSION   XP_034195598
VERSION     XP_034195598.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339707.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="lysophospholipid acyltransferase 6"
                     /calculated_mol_wt=57370
     Region          14..485
                     /region_name="MBOAT"
                     /note="membrane-bound O-acyltransferase family; cl00738"
                     /db_xref="CDD:445070"
     CDS             1..501
                     /gene="oys"
                     /coded_by="XM_034339707.2:414..1919"
                     /db_xref="GeneID:117611664"
ORIGIN      
        1 mtaivedyyd gsrtfqrlad ligvpidqvn flvtqfsali lagilrnfls ptkvspttrh
       61 vfglvnglll gyfcfgrqai hlaglpilcy ivmctqnprn mqnfvlatal iylscvhyhr
      121 qiynygsytl ditgplmvit qkvtslvynv hdglmrseee ltptqrhqav kkmpmvleyf
      181 syvfhfqalm agpviyyrdy mdfiygrmps klddpkdndk nsndravivn ppsptwvvik
      241 kvlasllcal mfvifipayp iqrlkeddfl ysttvfykiw yliisttlvr fkyyhawifa
      301 daicnnsglg fsgynekgka vwdatsnvdi fgfevsqslk msiehwnkgt nrwlrsvvye
      361 rvkhnkliyt yvlsaiwhgf ypgyyltfan gafitmvsrt arrkirpyfl gskgkkflyd
      421 tisfiatrii mayitfsfvl lefipsikvy lslymfphiv giailliapr lpkvpsqtss
      481 tetksnsnsq elingtarks m
//
LOCUS       XP_034195599             494 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase CSK isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195599
VERSION     XP_034195599.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339708.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="tyrosine-protein kinase CSK isoform X1"
                     /calculated_mol_wt=55412
     Region          117..214
                     /region_name="SH2_csk_like"
                     /note="Src homology 2 (SH2) domain found in
                     Carboxyl-Terminal Src Kinase (Csk); cd09937"
                     /db_xref="CDD:198190"
     Site            order(128,146,167,169)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198190"
     Site            order(168,195)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198190"
     Region          230..475
                     /region_name="PTKc_Csk_like"
                     /note="Catalytic domain of C-terminal Src kinase-like
                     Protein Tyrosine Kinases; cd05039"
                     /db_xref="CDD:270635"
     Site            order(243..245,251,262,264,292,308..311,314..315,356,
                     360..361,363,373..374,387..391,400,434)
                     /site_type="active"
                     /db_xref="CDD:270635"
     Site            order(243..245,251,262,264,292,308..311,314..315,360..361,
                     363,373..374)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270635"
     Site            order(257,268,281,284..285,287,301)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270635"
     Site            order(356,360,387..391,400,434)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270635"
     Site            373..393
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270635"
     CDS             1..494
                     /gene="LOC117611665"
                     /coded_by="XM_034339708.2:291..1775"
                     /db_xref="GeneID:117611665"
ORIGIN      
        1 mptyhnaggg ypnvsaaarq akldgnqnhh tipsnvtshp liqnstqhnv psnlsasnip
       61 shpvsptmtt hsnvtspnit shmntgsppv iltstnlanp snpavlsanp rhevklnamp
      121 wfhgkisret aerllrpred glflvrestn fpgdytlcvc yqgrvqhyrv kyknnqltid
      181 deeffenlal lvehyeqdad glctqltksl pkqgkqdfcv dpkafveagw viqthelelr
      241 ecigkgefgd vllgvyrger vavkmlkdns eaaqrflaea slmtslihdn lvkllglvfn
      301 nqhmylvtey mskgslvdyl rsrgrlhvsk kdqinfaydt cagmaylesr hvvhrdlaar
      361 nvlvaednsa kvsdfglard enfsleggkl pikwtapeal kqnkfsnksd mwsfgillwe
      421 iysfgrvpyp ripladvvkc vekgykmepp dgcphevydi mrqawdlqpe krptfydiki
      481 vlgqlkaeyt kgvn
//
LOCUS       XP_034195600             494 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase CSK isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195600
VERSION     XP_034195600.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339709.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="tyrosine-protein kinase CSK isoform X1"
                     /calculated_mol_wt=55412
     Region          117..214
                     /region_name="SH2_csk_like"
                     /note="Src homology 2 (SH2) domain found in
                     Carboxyl-Terminal Src Kinase (Csk); cd09937"
                     /db_xref="CDD:198190"
     Site            order(128,146,167,169)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198190"
     Site            order(168,195)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198190"
     Region          230..475
                     /region_name="PTKc_Csk_like"
                     /note="Catalytic domain of C-terminal Src kinase-like
                     Protein Tyrosine Kinases; cd05039"
                     /db_xref="CDD:270635"
     Site            order(243..245,251,262,264,292,308..311,314..315,356,
                     360..361,363,373..374,387..391,400,434)
                     /site_type="active"
                     /db_xref="CDD:270635"
     Site            order(243..245,251,262,264,292,308..311,314..315,360..361,
                     363,373..374)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270635"
     Site            order(257,268,281,284..285,287,301)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270635"
     Site            order(356,360,387..391,400,434)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270635"
     Site            373..393
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270635"
     CDS             1..494
                     /gene="LOC117611665"
                     /coded_by="XM_034339709.2:248..1732"
                     /db_xref="GeneID:117611665"
ORIGIN      
        1 mptyhnaggg ypnvsaaarq akldgnqnhh tipsnvtshp liqnstqhnv psnlsasnip
       61 shpvsptmtt hsnvtspnit shmntgsppv iltstnlanp snpavlsanp rhevklnamp
      121 wfhgkisret aerllrpred glflvrestn fpgdytlcvc yqgrvqhyrv kyknnqltid
      181 deeffenlal lvehyeqdad glctqltksl pkqgkqdfcv dpkafveagw viqthelelr
      241 ecigkgefgd vllgvyrger vavkmlkdns eaaqrflaea slmtslihdn lvkllglvfn
      301 nqhmylvtey mskgslvdyl rsrgrlhvsk kdqinfaydt cagmaylesr hvvhrdlaar
      361 nvlvaednsa kvsdfglard enfsleggkl pikwtapeal kqnkfsnksd mwsfgillwe
      421 iysfgrvpyp ripladvvkc vekgykmepp dgcphevydi mrqawdlqpe krptfydiki
      481 vlgqlkaeyt kgvn
//
LOCUS       XP_034195601             493 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein kinase CSK isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195601
VERSION     XP_034195601.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339710.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="tyrosine-protein kinase CSK isoform X2"
                     /calculated_mol_wt=55284
     Region          117..214
                     /region_name="SH2_csk_like"
                     /note="Src homology 2 (SH2) domain found in
                     Carboxyl-Terminal Src Kinase (Csk); cd09937"
                     /db_xref="CDD:198190"
     Site            order(128,146,167,169)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198190"
     Site            order(168,195)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198190"
     Region          230..474
                     /region_name="PTKc_Csk_like"
                     /note="Catalytic domain of C-terminal Src kinase-like
                     Protein Tyrosine Kinases; cd05039"
                     /db_xref="CDD:270635"
     Site            order(243..245,251,262,264,292,308..311,314..315,356,
                     360..361,363,373..374,387..391,400,433)
                     /site_type="active"
                     /db_xref="CDD:270635"
     Site            order(243..245,251,262,264,292,308..311,314..315,360..361,
                     363,373..374)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270635"
     Site            order(257,268,281,284..285,287,301)
                     /site_type="other"
                     /note="SH3/SH2 domain interface [polypeptide binding]"
                     /db_xref="CDD:270635"
     Site            order(356,360,387..391,400,433)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270635"
     Site            373..393
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270635"
     CDS             1..493
                     /gene="LOC117611665"
                     /coded_by="XM_034339710.2:292..1773"
                     /db_xref="GeneID:117611665"
ORIGIN      
        1 mptyhnaggg ypnvsaaarq akldgnqnhh tipsnvtshp liqnstqhnv psnlsasnip
       61 shpvsptmtt hsnvtspnit shmntgsppv iltstnlanp snpavlsanp rhevklnamp
      121 wfhgkisret aerllrpred glflvrestn fpgdytlcvc yqgrvqhyrv kyknnqltid
      181 deeffenlal lvehyeqdad glctqltksl pkqgkqdfcv dpkafveagw viqthelelr
      241 ecigkgefgd vllgvyrger vavkmlkdns eaaqrflaea slmtslihdn lvkllglvfn
      301 nqhmylvtey mskgslvdyl rsrgrlhvsk kdqinfaydt cagmaylesr hvvhrdlaar
      361 nvlvaednsa kvsdfglard enfsleggkl pikwtapeal kqnfsnksdm wsfgillwei
      421 ysfgrvpypr ipladvvkcv ekgykmeppd gcphevydim rqawdlqpek rptfydikiv
      481 lgqlkaeytk gvn
//
LOCUS       XP_034195603             361 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611667 [Osmia lignaria lignaria].
ACCESSION   XP_034195603
VERSION     XP_034195603.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339712.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..361
                     /product="uncharacterized protein LOC117611667"
                     /calculated_mol_wt=41337
     Region          52..281
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..361
                     /gene="LOC117611667"
                     /coded_by="XM_034339712.2:410..1495"
                     /db_xref="GeneID:117611667"
ORIGIN      
        1 mksheawers iefiehhlip emvqdrcfcv pnsrefvele savvrldevs rtseiyrati
       61 vllfsgepct isllvkllpe gmeqntiafd afqneemfys kmtlqygtdf vpksylsdlg
      121 rygrpvlvfe dldaagylqv dgqldddhlk lcakllgkfh atglrlkase pnifrefqak
      181 lleivlneet ieryekrssr mtdilesmpn srltgevkrr ldnspvetlk nvlgdvndvs
      241 tichghfshd nllfkyengk pidakvidwq tmrycspavd lgpillynvs hengpskvqe
      301 iltlyvdtvk seypevpgek lrediadkfi fvcfvlslqe hitddeltri lllveklyds
      361 v
//
LOCUS       XP_034195605             287 aa            linear   INV 03-SEP-2025
DEFINITION  zwei Ig domain protein zig-8 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195605
VERSION     XP_034195605.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339714.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="zwei Ig domain protein zig-8 isoform X1"
                     /calculated_mol_wt=31693
     Region          60..157
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          71..75
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          88..92
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..128
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          138..143
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          168..255
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          178..182
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:143220"
     Region          193..197
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:143220"
     Region          221..228
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:143220"
     Region          238..243
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:143220"
     CDS             1..287
                     /gene="LOC117611668"
                     /coded_by="XM_034339714.2:1320..2183"
                     /db_xref="GeneID:117611668"
ORIGIN      
        1 msavsvaawl lsavafaain neahcysmlh gssgkngnah qnslddsstp sgpyfdians
       61 knvtallgkt tylncrvknl gnrtmtlqvs wmrhrdvhll tnghytytnd qrfraihhah
      121 tddwmlqiky pqlrdsgfye cqvsttphms hivhlnviep kteilgapdl yinrgstinl
      181 tcvvlqspep payifwnhnd aiisydstrg gvsvvtekgd tttsfllvqe akpsdsgryt
      241 cnpsnaqpks itvhvlngey paamqhggqa kqprlyslll clcllly
//
LOCUS       XP_034195606             286 aa            linear   INV 03-SEP-2025
DEFINITION  zwei Ig domain protein zig-8 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195606
VERSION     XP_034195606.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339715.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="zwei Ig domain protein zig-8 isoform X2"
                     /calculated_mol_wt=31636
     Region          60..157
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          71..75
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          88..92
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..128
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          138..143
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          168..255
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          178..182
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:143220"
     Region          193..197
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:143220"
     Region          221..228
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:143220"
     Region          238..243
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:143220"
     CDS             1..286
                     /gene="LOC117611668"
                     /coded_by="XM_034339715.2:1320..2180"
                     /db_xref="GeneID:117611668"
ORIGIN      
        1 msavsvaawl lsavafaain neahcysmlh gssgkngnah qnslddsstp sgpyfdians
       61 knvtallgkt tylncrvknl gnrtmtlqvs wmrhrdvhll tnghytytnd qrfraihhah
      121 tddwmlqiky pqlrdsgfye cqvsttphms hivhlnviep kteilgapdl yinrgstinl
      181 tcvvlqspep payifwnhnd aiisydstrg gvsvvtekgd tttsfllvqe akpsdsgryt
      241 cnpsnaqpks itvhvlneyp aamqhggqak qprlyslllc lcllly
//
LOCUS       XP_034195607             283 aa            linear   INV 03-SEP-2025
DEFINITION  zwei Ig domain protein zig-8 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195607
VERSION     XP_034195607.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339716.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="zwei Ig domain protein zig-8 isoform X3"
                     /calculated_mol_wt=31219
     Region          60..153
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          71..75
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          84..88
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          120..124
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          134..139
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          164..251
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          174..178
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:143220"
     Region          189..193
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:143220"
     Region          217..224
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:143220"
     Region          234..239
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:143220"
     CDS             1..283
                     /gene="LOC117611668"
                     /coded_by="XM_034339716.2:1324..2175"
                     /db_xref="GeneID:117611668"
ORIGIN      
        1 msavsvaawl lsavafaain neahcysmlh gssgkngnah qnslddsstp sgpyfdians
       61 knvtallgkt tylncrvknl gnrtvswmrh rdvhlltngh ytytndqrfr aihhahtddw
      121 mlqikypqlr dsgfyecqvs ttphmshivh lnviepktei lgapdlyinr gstinltcvv
      181 lqspeppayi fwnhndaiis ydstrggvsv vtekgdttts fllvqeakps dsgrytcnps
      241 naqpksitvh vlngeypaam qhggqakqpr lyslllclcl lly
//
LOCUS       XP_034195608             282 aa            linear   INV 03-SEP-2025
DEFINITION  zwei Ig domain protein zig-8 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195608
VERSION     XP_034195608.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339717.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="zwei Ig domain protein zig-8 isoform X4"
                     /calculated_mol_wt=31162
     Region          60..153
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          71..75
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          84..88
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          120..124
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          134..139
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          164..251
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          174..178
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:143220"
     Region          189..193
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:143220"
     Region          217..224
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:143220"
     Region          234..239
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:143220"
     CDS             1..282
                     /gene="LOC117611668"
                     /coded_by="XM_034339717.2:1324..2172"
                     /db_xref="GeneID:117611668"
ORIGIN      
        1 msavsvaawl lsavafaain neahcysmlh gssgkngnah qnslddsstp sgpyfdians
       61 knvtallgkt tylncrvknl gnrtvswmrh rdvhlltngh ytytndqrfr aihhahtddw
      121 mlqikypqlr dsgfyecqvs ttphmshivh lnviepktei lgapdlyinr gstinltcvv
      181 lqspeppayi fwnhndaiis ydstrggvsv vtekgdttts fllvqeakps dsgrytcnps
      241 naqpksitvh vlneypaamq hggqakqprl yslllclcll ly
//
LOCUS       XP_034195609             154 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611669 [Osmia lignaria lignaria].
ACCESSION   XP_034195609
VERSION     XP_034195609.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339718.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..154
                     /product="uncharacterized protein LOC117611669"
                     /calculated_mol_wt=16583
     Region          <75..148
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..154
                     /gene="LOC117611669"
                     /coded_by="XM_034339718.2:691..1155"
                     /db_xref="GeneID:117611669"
ORIGIN      
        1 mgltavkalc dcngtslstr qtlecyvsgk dllpscvpil katvrtrcsa rrmlisscil
       61 iiimaiiycm sfanlrrpsl gipfrrqsdp qpaadshsps ettiqasent tediaediae
      121 diaediaedt aedtaedade adtengdeep pqee
//
LOCUS       XP_034195610             149 aa            linear   INV 03-SEP-2025
DEFINITION  hymenoptaecin [Osmia lignaria lignaria].
ACCESSION   XP_034195610
VERSION     XP_034195610.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339719.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..149
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..149
                     /product="hymenoptaecin"
                     /calculated_mol_wt=16320
     CDS             1..149
                     /gene="LOC117611670"
                     /coded_by="XM_034339719.2:103..552"
                     /db_xref="GeneID:117611670"
ORIGIN      
        1 mkfialaval fcavayvtae fgeeqllety npvrfrrean pqgsvvirge kplsgpdrrp
       61 sldidyqqri idrnglnana ygglnvrpgq saqphvglna errfkdgfig gfgqvqrgpg
      121 grpsptfgln ggfrfrrdvd evveqgeee
//
LOCUS       XP_034195611              90 aa            linear   INV 03-SEP-2025
DEFINITION  translocase of inner membrane 8 [Osmia lignaria lignaria].
ACCESSION   XP_034195611
VERSION     XP_034195611.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339720.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..90
                     /product="translocase of inner membrane 8"
                     /calculated_mol_wt=10130
     Region          20..82
                     /region_name="zf-Tim10_DDP"
                     /note="Tim10/DDP family zinc finger; pfam02953"
                     /db_xref="CDD:460764"
     CDS             1..90
                     /gene="Tim8"
                     /coded_by="XM_034339720.2:153..425"
                     /db_xref="GeneID:117611671"
ORIGIN      
        1 msdsslqdsq iagadselqe fflaekqkaq fqaqirefnd icwdkcvekp gvkldsrtet
       61 clsncvdrfi dvsllitnrf gqvlqkvlen
//
LOCUS       XP_076546719              90 aa            linear   INV 03-SEP-2025
DEFINITION  translocase of inner membrane 8 [Osmia lignaria lignaria].
ACCESSION   XP_076546719
VERSION     XP_076546719.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690604.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..90
                     /product="translocase of inner membrane 8"
                     /calculated_mol_wt=10130
     Region          20..82
                     /region_name="zf-Tim10_DDP"
                     /note="Tim10/DDP family zinc finger; pfam02953"
                     /db_xref="CDD:460764"
     CDS             1..90
                     /gene="Tim8"
                     /coded_by="XM_076690604.1:192..464"
                     /db_xref="GeneID:117611671"
ORIGIN      
        1 msdsslqdsq iagadselqe fflaekqkaq fqaqirefnd icwdkcvekp gvkldsrtet
       61 clsncvdrfi dvsllitnrf gqvlqkvlen
//
LOCUS       XP_034194650             104 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610909 [Osmia lignaria lignaria].
ACCESSION   XP_034194650
VERSION     XP_034194650.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338759.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..104
                     /product="uncharacterized protein LOC117610909"
                     /calculated_mol_wt=12165
     CDS             1..104
                     /gene="LOC117610909"
                     /coded_by="XM_034338759.2:1884..2198"
                     /db_xref="GeneID:117610909"
ORIGIN      
        1 mrtnnitecy nnivllkmgk gkkniwvfle kleqlimdee ikikrlnegq cvcrksnkkt
       61 iirnkrieti qkdlanrrlt ltesllsfyk gnkiliygdy egkv
//
LOCUS       XP_034195564            2901 aa            linear   INV 03-SEP-2025
DEFINITION  teneurin-a transmembrane protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195564
VERSION     XP_034195564.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339673.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2901
                     /product="teneurin-a transmembrane protein isoform X1"
                     /calculated_mol_wt=320382
     Region          616..639
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          661..794
                     /region_name="C_rich_MXAN6577"
                     /note="MXAN_6577-like cysteine-rich domain; NF041328"
                     /db_xref="CDD:469225"
     Region          796..839
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     Region          878..904
                     /region_name="acid_disulf_rpt"
                     /note="acidic double-disulfide repeat; NF033662"
                     /db_xref="CDD:411265"
     Region          1292..1649
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          1345..1391
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1415..1457
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1472..1511
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1545..1578
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1612..2575
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          2796..2873
                     /region_name="Tox-GHH"
                     /note="GHH signature containing HNH/Endo VII superfamily
                     nuclease toxin; pfam15636"
                     /db_xref="CDD:464783"
     CDS             1..2901
                     /gene="Ten-a"
                     /coded_by="XM_034339673.2:1136..9841"
                     /db_xref="GeneID:117611646"
ORIGIN      
        1 mdsegtlgrg hgnggrglrr qrslewrerr gygpsaqsss ddeensrhav gptstssagr
       61 garspgqvfa sllaqqygsv gdrsyrtgsd tegaattdnp ttpfptpppt ltpnhrqasp
      121 fplestnsrr hhyngsisse rsrngngetv yaapskktnt gggtlgrrtr rghtsalsss
      181 stasdrsetv fpdeharmhl tndtgrdspp epappevppr gpslhathtl rtqqnrngcp
      241 pnatgnfavp tdqmqtekys eylmsgnprs yparspgvas tptvtrlppp qqqvtvgsvg
      301 gglgagagng gtgtvgaqgq amvmpgfplr aaavphyspy spsrfhidkr cqhrcswkcf
      361 sialillava ltamlayfat vssmrpidst kcvvvqdvka vthenahihd pistqiptee
      421 slptstaehs sasdsagdqq sdpqiqqsaq qwpavlelqa ynvahsavip pyhfwntefr
      481 nkqpafirln ltlpwganfa vygrrnvaps vtqydfvefv kggrvdhrlk rdtsseavsf
      541 mktaaqetht nvenrsyqhv likrtivepm mvnvsllqyl dtgrwflsvy ndelqpykvt
      601 lvvteaegvs ttcpndcsgr gscylgkcdc idgfqgadcs ksvcpvlcss hgqygggmch
      661 cedgwkgaec diplgdcqvp dcnqhgqcvr gscvcnpgwk gvfcdepdcs dpncsghgac
      721 vsgkcyckag wqgercnqvd qqvyqclprc sdhgtydles atciceghwt gvdcsqpscg
      781 ldcgphgtce qgvckcnddw tgtkcdqkpc dprcaehgqc kngicvcsqg wngrhctlpg
      841 cengcsrhgl ctlqdgeysc ecstgwagrd csirlemecn dfidndqdgm mdcsdseccs
      901 haacadhimc ltsnnpvdvl lrkqppsvta sfyqrvkflv eensvqsyah mneytestfw
      961 ssftprrvav mrgqvvteqg igivgirvsv drdprfgftl tradgwfdvl vngggavtlq
     1021 fqrspfkplt ttvfvpwnqi vvlppvvmtl neegeshksn qppspavgfp gismglfsdh
     1081 gpclehdhel lrpiivstwm pekvgdlpgk slvfaesqiv qesiaipgsn lhlmyqssqa
     1141 agylstvrmq ltgsfipksl thvhvhveie gslhtktyea dpnlthtfaw nkrnvykqkv
     1201 ygvaqarisi gyqhstcpsv iwetqtatlq gfdvdisdig gwgldihhhy nfhegilqkg
     1261 dgttlhfkqy prtvkvvmgt glqrslvcqd cdglakdarl ltpvaltsgp dgsiyvgdfn
     1321 lvrritpegn vytvltlsat qvayqyylcv spadghlyis dpekhqilka lslknvqhps
     1381 lniepvvgsg ercipgdesh cgdegpaira klahpkgiai aadktmyiad grniravdpn
     1441 gvihtlvghh ghhnhwspap cvgaipahqa qlqwptgltl spldgslhfi ddrlvlklts
     1501 dlkvrvvagt plhctsngng ndsdltkvns siatipkkpd evlgsvlavs fsptgelyia
     1561 esdshrvnsi kivdssgkms hfagqqqerl rgqcecnntt pstkelesca ctddtsstet
     1621 llssnakfta isalavspdg vlhvadqgsl hilalqpylp nhdesgefhi pfppsnevyv
     1681 fnrygqhist kdltsgkiry sffyskntsf gklstvtdsa gnkiqfwrdy sgvvnsient
     1741 qdhkselkis avgfleklse rgraeialay dgstglltsr sgggetyiyn ydslgrvtdv
     1801 ilptgeklkl ssdlsddegl lvkvsaplqa lskgdkqrtv efrmknersk vltitdgtki
     1861 kkaiaftnss levllpgggr vlsaattkhp lleaalpvea emlpmwsyql mslgeltntm
     1921 ttaynlvgdv shfqqtlnre iwvndtrvig vefeqafrre tfydksrnpl ltvtfdpagl
     1981 pltwqpheqg ynlsitydrf nrieswkwgg sdesygydrh gqlaevtnsq dgtkrytynd
     2041 fnilsnitla sdrhfslqyd ddgglrhiil psgtkhsfsc qaslgflrvt ytppgssray
     2101 lqhyshdgnl lqtvfpgdga rivyryhssg qlaevvhgdg kseirytesg lpsevihser
     2161 dveykwdyqy sagllveeri dfgaktglsn akftfdydsn frlinvqgri ggqtlpphtm
     2221 aysartgmle qigqfkvtkp qtnettvfdg talfsritdd rfletqvtlt ihqlevfrme
     2281 fthdsrgrin qtrtytrnva vntytnvkny twdcdgqltg veaqepwgfk ydangnmlsl
     2341 tyrgntipme ynnmdrivkf geglykydnr glvvqnaree rfsynakgll vraakrgrfd
     2401 vryyydhldr latrkdnygn vtqffynnqk rphevsqiys prdgklmslv yddrghliya
     2461 qvyrhkyyia tdqcgtpimi fsqygegire imrspyghiv ydsnpylylp vdfcgglldq
     2521 vtslvhmpng kvydpligkw msplwenvld rvntpthlhl yrfngndpid vrhtdrpnqp
     2581 tdhiswlshl gydlkslapq lfpdelpdsl vppslgpgts ifgkkkktrn lgvqsgflah
     2641 iaqrhsgdav slsapprsal kkdtsdlips rlgaasdppf gkgilvsrtm dgqavvssvp
     2701 tanaiygdvf tsvfnrsyfl pftfvvhsaq qdafyfvkee twrasedrgq lkrlggqvnt
     2761 tfhesengsg ssslidvkih gasyvvnlry gttadkekqr llhhakatai rkawhrerea
     2821 lkantpttie wmvteqdeil ksgsasnyeg eyihdaqlyp elaedpynir fvkkavdpss
     2881 kkrrrrrgvh tcklwwldki c
//
LOCUS       XP_076546720            2901 aa            linear   INV 03-SEP-2025
DEFINITION  teneurin-a transmembrane protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546720
VERSION     XP_076546720.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690605.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2901
                     /product="teneurin-a transmembrane protein isoform X1"
                     /calculated_mol_wt=320382
     Region          616..639
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          661..794
                     /region_name="C_rich_MXAN6577"
                     /note="MXAN_6577-like cysteine-rich domain; NF041328"
                     /db_xref="CDD:469225"
     Region          796..839
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     Region          878..904
                     /region_name="acid_disulf_rpt"
                     /note="acidic double-disulfide repeat; NF033662"
                     /db_xref="CDD:411265"
     Region          1292..1649
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          1345..1391
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1415..1457
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1472..1511
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1545..1578
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1612..2575
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          2796..2873
                     /region_name="Tox-GHH"
                     /note="GHH signature containing HNH/Endo VII superfamily
                     nuclease toxin; pfam15636"
                     /db_xref="CDD:464783"
     CDS             1..2901
                     /gene="Ten-a"
                     /coded_by="XM_076690605.1:1100..9805"
                     /db_xref="GeneID:117611646"
ORIGIN      
        1 mdsegtlgrg hgnggrglrr qrslewrerr gygpsaqsss ddeensrhav gptstssagr
       61 garspgqvfa sllaqqygsv gdrsyrtgsd tegaattdnp ttpfptpppt ltpnhrqasp
      121 fplestnsrr hhyngsisse rsrngngetv yaapskktnt gggtlgrrtr rghtsalsss
      181 stasdrsetv fpdeharmhl tndtgrdspp epappevppr gpslhathtl rtqqnrngcp
      241 pnatgnfavp tdqmqtekys eylmsgnprs yparspgvas tptvtrlppp qqqvtvgsvg
      301 gglgagagng gtgtvgaqgq amvmpgfplr aaavphyspy spsrfhidkr cqhrcswkcf
      361 sialillava ltamlayfat vssmrpidst kcvvvqdvka vthenahihd pistqiptee
      421 slptstaehs sasdsagdqq sdpqiqqsaq qwpavlelqa ynvahsavip pyhfwntefr
      481 nkqpafirln ltlpwganfa vygrrnvaps vtqydfvefv kggrvdhrlk rdtsseavsf
      541 mktaaqetht nvenrsyqhv likrtivepm mvnvsllqyl dtgrwflsvy ndelqpykvt
      601 lvvteaegvs ttcpndcsgr gscylgkcdc idgfqgadcs ksvcpvlcss hgqygggmch
      661 cedgwkgaec diplgdcqvp dcnqhgqcvr gscvcnpgwk gvfcdepdcs dpncsghgac
      721 vsgkcyckag wqgercnqvd qqvyqclprc sdhgtydles atciceghwt gvdcsqpscg
      781 ldcgphgtce qgvckcnddw tgtkcdqkpc dprcaehgqc kngicvcsqg wngrhctlpg
      841 cengcsrhgl ctlqdgeysc ecstgwagrd csirlemecn dfidndqdgm mdcsdseccs
      901 haacadhimc ltsnnpvdvl lrkqppsvta sfyqrvkflv eensvqsyah mneytestfw
      961 ssftprrvav mrgqvvteqg igivgirvsv drdprfgftl tradgwfdvl vngggavtlq
     1021 fqrspfkplt ttvfvpwnqi vvlppvvmtl neegeshksn qppspavgfp gismglfsdh
     1081 gpclehdhel lrpiivstwm pekvgdlpgk slvfaesqiv qesiaipgsn lhlmyqssqa
     1141 agylstvrmq ltgsfipksl thvhvhveie gslhtktyea dpnlthtfaw nkrnvykqkv
     1201 ygvaqarisi gyqhstcpsv iwetqtatlq gfdvdisdig gwgldihhhy nfhegilqkg
     1261 dgttlhfkqy prtvkvvmgt glqrslvcqd cdglakdarl ltpvaltsgp dgsiyvgdfn
     1321 lvrritpegn vytvltlsat qvayqyylcv spadghlyis dpekhqilka lslknvqhps
     1381 lniepvvgsg ercipgdesh cgdegpaira klahpkgiai aadktmyiad grniravdpn
     1441 gvihtlvghh ghhnhwspap cvgaipahqa qlqwptgltl spldgslhfi ddrlvlklts
     1501 dlkvrvvagt plhctsngng ndsdltkvns siatipkkpd evlgsvlavs fsptgelyia
     1561 esdshrvnsi kivdssgkms hfagqqqerl rgqcecnntt pstkelesca ctddtsstet
     1621 llssnakfta isalavspdg vlhvadqgsl hilalqpylp nhdesgefhi pfppsnevyv
     1681 fnrygqhist kdltsgkiry sffyskntsf gklstvtdsa gnkiqfwrdy sgvvnsient
     1741 qdhkselkis avgfleklse rgraeialay dgstglltsr sgggetyiyn ydslgrvtdv
     1801 ilptgeklkl ssdlsddegl lvkvsaplqa lskgdkqrtv efrmknersk vltitdgtki
     1861 kkaiaftnss levllpgggr vlsaattkhp lleaalpvea emlpmwsyql mslgeltntm
     1921 ttaynlvgdv shfqqtlnre iwvndtrvig vefeqafrre tfydksrnpl ltvtfdpagl
     1981 pltwqpheqg ynlsitydrf nrieswkwgg sdesygydrh gqlaevtnsq dgtkrytynd
     2041 fnilsnitla sdrhfslqyd ddgglrhiil psgtkhsfsc qaslgflrvt ytppgssray
     2101 lqhyshdgnl lqtvfpgdga rivyryhssg qlaevvhgdg kseirytesg lpsevihser
     2161 dveykwdyqy sagllveeri dfgaktglsn akftfdydsn frlinvqgri ggqtlpphtm
     2221 aysartgmle qigqfkvtkp qtnettvfdg talfsritdd rfletqvtlt ihqlevfrme
     2281 fthdsrgrin qtrtytrnva vntytnvkny twdcdgqltg veaqepwgfk ydangnmlsl
     2341 tyrgntipme ynnmdrivkf geglykydnr glvvqnaree rfsynakgll vraakrgrfd
     2401 vryyydhldr latrkdnygn vtqffynnqk rphevsqiys prdgklmslv yddrghliya
     2461 qvyrhkyyia tdqcgtpimi fsqygegire imrspyghiv ydsnpylylp vdfcgglldq
     2521 vtslvhmpng kvydpligkw msplwenvld rvntpthlhl yrfngndpid vrhtdrpnqp
     2581 tdhiswlshl gydlkslapq lfpdelpdsl vppslgpgts ifgkkkktrn lgvqsgflah
     2641 iaqrhsgdav slsapprsal kkdtsdlips rlgaasdppf gkgilvsrtm dgqavvssvp
     2701 tanaiygdvf tsvfnrsyfl pftfvvhsaq qdafyfvkee twrasedrgq lkrlggqvnt
     2761 tfhesengsg ssslidvkih gasyvvnlry gttadkekqr llhhakatai rkawhrerea
     2821 lkantpttie wmvteqdeil ksgsasnyeg eyihdaqlyp elaedpynir fvkkavdpss
     2881 kkrrrrrgvh tcklwwldki c
//
LOCUS       XP_034195565            2900 aa            linear   INV 03-SEP-2025
DEFINITION  teneurin-a transmembrane protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195565
VERSION     XP_034195565.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339674.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2900
                     /product="teneurin-a transmembrane protein isoform X2"
                     /calculated_mol_wt=320313
     Region          615..638
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          660..793
                     /region_name="C_rich_MXAN6577"
                     /note="MXAN_6577-like cysteine-rich domain; NF041328"
                     /db_xref="CDD:469225"
     Region          795..838
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     Region          877..903
                     /region_name="acid_disulf_rpt"
                     /note="acidic double-disulfide repeat; NF033662"
                     /db_xref="CDD:411265"
     Region          1291..1648
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          1344..1390
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1414..1456
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1471..1510
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1544..1577
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1611..2574
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          2795..2872
                     /region_name="Tox-GHH"
                     /note="GHH signature containing HNH/Endo VII superfamily
                     nuclease toxin; pfam15636"
                     /db_xref="CDD:464783"
     CDS             1..2900
                     /gene="Ten-a"
                     /coded_by="XM_034339674.2:1136..9838"
                     /db_xref="GeneID:117611646"
ORIGIN      
        1 mdsegtlgrg hgnggrglrr qrslewrerr gygpsaqsss ddeensrhav gptstssagr
       61 garspgqvfa sllaqqygsv gdrsyrtgsd tegaattdnp ttpfptpppt ltpnhrqasp
      121 fplestnsrr hhyngsisse rsrngngetv yaapskktnt gggtlgrrtr rghtsalsss
      181 stasdrsetv fpdeharmhl tndtgrdspp epappevppr gpslhathtl rtqqnrngcp
      241 pnatgnfavp tdqmqtekys eylmsgnprs yparspgvas tptvtrlppp qqqvtvgsvg
      301 gglgagagng gtgtvgaqgq amvmpgfplr aaavphyspy spsrfhidkr cqhrcswkcf
      361 sialillava ltamlayfam ssmrpidstk cvvvqdvkav thenahihdp istqiptees
      421 lptstaehss asdsagdqqs dpqiqqsaqq wpavlelqay nvahsavipp yhfwntefrn
      481 kqpafirlnl tlpwganfav ygrrnvapsv tqydfvefvk ggrvdhrlkr dtsseavsfm
      541 ktaaqethtn venrsyqhvl ikrtivepmm vnvsllqyld tgrwflsvyn delqpykvtl
      601 vvteaegvst tcpndcsgrg scylgkcdci dgfqgadcsk svcpvlcssh gqygggmchc
      661 edgwkgaecd iplgdcqvpd cnqhgqcvrg scvcnpgwkg vfcdepdcsd pncsghgacv
      721 sgkcyckagw qgercnqvdq qvyqclprcs dhgtydlesa tciceghwtg vdcsqpscgl
      781 dcgphgtceq gvckcnddwt gtkcdqkpcd prcaehgqck ngicvcsqgw ngrhctlpgc
      841 engcsrhglc tlqdgeysce cstgwagrdc sirlemecnd fidndqdgmm dcsdseccsh
      901 aacadhimcl tsnnpvdvll rkqppsvtas fyqrvkflve ensvqsyahm neytestfws
      961 sftprrvavm rgqvvteqgi givgirvsvd rdprfgftlt radgwfdvlv ngggavtlqf
     1021 qrspfkpltt tvfvpwnqiv vlppvvmtln eegeshksnq ppspavgfpg ismglfsdhg
     1081 pclehdhell rpiivstwmp ekvgdlpgks lvfaesqivq esiaipgsnl hlmyqssqaa
     1141 gylstvrmql tgsfipkslt hvhvhveieg slhtktyead pnlthtfawn krnvykqkvy
     1201 gvaqarisig yqhstcpsvi wetqtatlqg fdvdisdigg wgldihhhyn fhegilqkgd
     1261 gttlhfkqyp rtvkvvmgtg lqrslvcqdc dglakdarll tpvaltsgpd gsiyvgdfnl
     1321 vrritpegnv ytvltlsatq vayqyylcvs padghlyisd pekhqilkal slknvqhpsl
     1381 niepvvgsge rcipgdeshc gdegpairak lahpkgiaia adktmyiadg rniravdpng
     1441 vihtlvghhg hhnhwspapc vgaipahqaq lqwptgltls pldgslhfid drlvlkltsd
     1501 lkvrvvagtp lhctsngngn dsdltkvnss iatipkkpde vlgsvlavsf sptgelyiae
     1561 sdshrvnsik ivdssgkmsh fagqqqerlr gqcecnnttp stkelescac tddtsstetl
     1621 lssnakftai salavspdgv lhvadqgslh ilalqpylpn hdesgefhip fppsnevyvf
     1681 nrygqhistk dltsgkirys ffyskntsfg klstvtdsag nkiqfwrdys gvvnsientq
     1741 dhkselkisa vgfleklser graeialayd gstglltsrs gggetyiyny dslgrvtdvi
     1801 lptgeklkls sdlsddegll vkvsaplqal skgdkqrtve frmknerskv ltitdgtkik
     1861 kaiaftnssl evllpgggrv lsaattkhpl leaalpveae mlpmwsyqlm slgeltntmt
     1921 taynlvgdvs hfqqtlnrei wvndtrvigv efeqafrret fydksrnpll tvtfdpaglp
     1981 ltwqpheqgy nlsitydrfn rieswkwggs desygydrhg qlaevtnsqd gtkrytyndf
     2041 nilsnitlas drhfslqydd dgglrhiilp sgtkhsfscq aslgflrvty tppgssrayl
     2101 qhyshdgnll qtvfpgdgar ivyryhssgq laevvhgdgk seirytesgl psevihserd
     2161 veykwdyqys agllveerid fgaktglsna kftfdydsnf rlinvqgrig gqtlpphtma
     2221 ysartgmleq igqfkvtkpq tnettvfdgt alfsritddr fletqvtlti hqlevfrmef
     2281 thdsrgrinq trtytrnvav ntytnvknyt wdcdgqltgv eaqepwgfky dangnmlslt
     2341 yrgntipmey nnmdrivkfg eglykydnrg lvvqnareer fsynakgllv raakrgrfdv
     2401 ryyydhldrl atrkdnygnv tqffynnqkr phevsqiysp rdgklmslvy ddrghliyaq
     2461 vyrhkyyiat dqcgtpimif sqygegirei mrspyghivy dsnpylylpv dfcgglldqv
     2521 tslvhmpngk vydpligkwm splwenvldr vntpthlhly rfngndpidv rhtdrpnqpt
     2581 dhiswlshlg ydlkslapql fpdelpdslv ppslgpgtsi fgkkkktrnl gvqsgflahi
     2641 aqrhsgdavs lsapprsalk kdtsdlipsr lgaasdppfg kgilvsrtmd gqavvssvpt
     2701 anaiygdvft svfnrsyflp ftfvvhsaqq dafyfvkeet wrasedrgql krlggqvntt
     2761 fhesengsgs sslidvkihg asyvvnlryg ttadkekqrl lhhakatair kawhrereal
     2821 kantpttiew mvteqdeilk sgsasnyege yihdaqlype laedpynirf vkkavdpssk
     2881 krrrrrgvht cklwwldkic
//
LOCUS       XP_034195566            2898 aa            linear   INV 03-SEP-2025
DEFINITION  teneurin-a transmembrane protein isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195566
VERSION     XP_034195566.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339675.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2898
                     /product="teneurin-a transmembrane protein isoform X3"
                     /calculated_mol_wt=320081
     Region          616..639
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          661..794
                     /region_name="C_rich_MXAN6577"
                     /note="MXAN_6577-like cysteine-rich domain; NF041328"
                     /db_xref="CDD:469225"
     Region          796..839
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     Region          878..904
                     /region_name="acid_disulf_rpt"
                     /note="acidic double-disulfide repeat; NF033662"
                     /db_xref="CDD:411265"
     Region          1289..1646
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          1342..1388
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1412..1454
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1469..1508
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1542..1575
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1609..2572
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          2793..2870
                     /region_name="Tox-GHH"
                     /note="GHH signature containing HNH/Endo VII superfamily
                     nuclease toxin; pfam15636"
                     /db_xref="CDD:464783"
     CDS             1..2898
                     /gene="Ten-a"
                     /coded_by="XM_034339675.2:1137..9833"
                     /db_xref="GeneID:117611646"
ORIGIN      
        1 mdsegtlgrg hgnggrglrr qrslewrerr gygpsaqsss ddeensrhav gptstssagr
       61 garspgqvfa sllaqqygsv gdrsyrtgsd tegaattdnp ttpfptpppt ltpnhrqasp
      121 fplestnsrr hhyngsisse rsrngngetv yaapskktnt gggtlgrrtr rghtsalsss
      181 stasdrsetv fpdeharmhl tndtgrdspp epappevppr gpslhathtl rtqqnrngcp
      241 pnatgnfavp tdqmqtekys eylmsgnprs yparspgvas tptvtrlppp qqqvtvgsvg
      301 gglgagagng gtgtvgaqgq amvmpgfplr aaavphyspy spsrfhidkr cqhrcswkcf
      361 sialillava ltamlayfat vssmrpidst kcvvvqdvka vthenahihd pistqiptee
      421 slptstaehs sasdsagdqq sdpqiqqsaq qwpavlelqa ynvahsavip pyhfwntefr
      481 nkqpafirln ltlpwganfa vygrrnvaps vtqydfvefv kggrvdhrlk rdtsseavsf
      541 mktaaqetht nvenrsyqhv likrtivepm mvnvsllqyl dtgrwflsvy ndelqpykvt
      601 lvvteaegvs ttcpndcsgr gscylgkcdc idgfqgadcs ksvcpvlcss hgqygggmch
      661 cedgwkgaec diplgdcqvp dcnqhgqcvr gscvcnpgwk gvfcdepdcs dpncsghgac
      721 vsgkcyckag wqgercnqvd qqvyqclprc sdhgtydles atciceghwt gvdcsqpscg
      781 ldcgphgtce qgvckcnddw tgtkcdqkpc dprcaehgqc kngicvcsqg wngrhctlpg
      841 cengcsrhgl ctlqdgeysc ecstgwagrd csirlemecn dfidndqdgm mdcsdseccs
      901 haacadhimc ltsnnpvdvl lrkqppsvta sfyqrvkflv eensvqsyah mneytestfw
      961 ssftprrvav mrgqvvteqg igivgirvsv drdprfgftl tradgwfdvl vngggavtlq
     1021 fqrspfkplt ttvfvpwnqi vvlppvvmtl neegeshksn qppspavgis mglfsdhgpc
     1081 lehdhellrp iivstwmpek vgdlpgkslv faesqivqes iaipgsnlhl myqssqaagy
     1141 lstvrmqltg sfipkslthv hvhveiegsl htktyeadpn lthtfawnkr nvykqkvygv
     1201 aqarisigyq hstcpsviwe tqtatlqgfd vdisdiggwg ldihhhynfh egilqkgdgt
     1261 tlhfkqyprt vkvvmgtglq rslvcqdcdg lakdarlltp valtsgpdgs iyvgdfnlvr
     1321 ritpegnvyt vltlsatqva yqyylcvspa dghlyisdpe khqilkalsl knvqhpslni
     1381 epvvgsgerc ipgdeshcgd egpairakla hpkgiaiaad ktmyiadgrn iravdpngvi
     1441 htlvghhghh nhwspapcvg aipahqaqlq wptgltlspl dgslhfiddr lvlkltsdlk
     1501 vrvvagtplh ctsngngnds dltkvnssia tipkkpdevl gsvlavsfsp tgelyiaesd
     1561 shrvnsikiv dssgkmshfa gqqqerlrgq cecnnttpst kelescactd dtsstetlls
     1621 snakftaisa lavspdgvlh vadqgslhil alqpylpnhd esgefhipfp psnevyvfnr
     1681 ygqhistkdl tsgkirysff yskntsfgkl stvtdsagnk iqfwrdysgv vnsientqdh
     1741 kselkisavg fleklsergr aeialaydgs tglltsrsgg getyiynyds lgrvtdvilp
     1801 tgeklklssd lsddegllvk vsaplqalsk gdkqrtvefr mknerskvlt itdgtkikka
     1861 iaftnsslev llpgggrvls aattkhplle aalpveaeml pmwsyqlmsl geltntmtta
     1921 ynlvgdvshf qqtlnreiwv ndtrvigvef eqafrretfy dksrnplltv tfdpaglplt
     1981 wqpheqgynl sitydrfnri eswkwggsde sygydrhgql aevtnsqdgt krytyndfni
     2041 lsnitlasdr hfslqydddg glrhiilpsg tkhsfscqas lgflrvtytp pgssraylqh
     2101 yshdgnllqt vfpgdgariv yryhssgqla evvhgdgkse irytesglps evihserdve
     2161 ykwdyqysag llveeridfg aktglsnakf tfdydsnfrl invqgriggq tlpphtmays
     2221 artgmleqig qfkvtkpqtn ettvfdgtal fsritddrfl etqvtltihq levfrmefth
     2281 dsrgrinqtr tytrnvavnt ytnvknytwd cdgqltgvea qepwgfkyda ngnmlsltyr
     2341 gntipmeynn mdrivkfgeg lykydnrglv vqnareerfs ynakgllvra akrgrfdvry
     2401 yydhldrlat rkdnygnvtq ffynnqkrph evsqiysprd gklmslvydd rghliyaqvy
     2461 rhkyyiatdq cgtpimifsq ygegireimr spyghivyds npylylpvdf cgglldqvts
     2521 lvhmpngkvy dpligkwmsp lwenvldrvn tpthlhlyrf ngndpidvrh tdrpnqptdh
     2581 iswlshlgyd lkslapqlfp delpdslvpp slgpgtsifg kkkktrnlgv qsgflahiaq
     2641 rhsgdavsls apprsalkkd tsdlipsrlg aasdppfgkg ilvsrtmdgq avvssvptan
     2701 aiygdvftsv fnrsyflpft fvvhsaqqda fyfvkeetwr asedrgqlkr lggqvnttfh
     2761 esengsgsss lidvkihgas yvvnlrygtt adkekqrllh hakatairka whrerealka
     2821 ntpttiewmv teqdeilksg sasnyegeyi hdaqlypela edpynirfvk kavdpsskkr
     2881 rrrrgvhtck lwwldkic
//
LOCUS       XP_034195567            2892 aa            linear   INV 03-SEP-2025
DEFINITION  teneurin-a transmembrane protein isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195567
VERSION     XP_034195567.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339676.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2892
                     /product="teneurin-a transmembrane protein isoform X4"
                     /calculated_mol_wt=319272
     Region          616..639
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          661..794
                     /region_name="C_rich_MXAN6577"
                     /note="MXAN_6577-like cysteine-rich domain; NF041328"
                     /db_xref="CDD:469225"
     Region          796..839
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     Region          878..904
                     /region_name="acid_disulf_rpt"
                     /note="acidic double-disulfide repeat; NF033662"
                     /db_xref="CDD:411265"
     Region          1283..1640
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          1336..1382
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1406..1448
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1463..1502
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1536..1569
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1603..2566
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          2787..2864
                     /region_name="Tox-GHH"
                     /note="GHH signature containing HNH/Endo VII superfamily
                     nuclease toxin; pfam15636"
                     /db_xref="CDD:464783"
     CDS             1..2892
                     /gene="Ten-a"
                     /coded_by="XM_034339676.2:1136..9814"
                     /db_xref="GeneID:117611646"
ORIGIN      
        1 mdsegtlgrg hgnggrglrr qrslewrerr gygpsaqsss ddeensrhav gptstssagr
       61 garspgqvfa sllaqqygsv gdrsyrtgsd tegaattdnp ttpfptpppt ltpnhrqasp
      121 fplestnsrr hhyngsisse rsrngngetv yaapskktnt gggtlgrrtr rghtsalsss
      181 stasdrsetv fpdeharmhl tndtgrdspp epappevppr gpslhathtl rtqqnrngcp
      241 pnatgnfavp tdqmqtekys eylmsgnprs yparspgvas tptvtrlppp qqqvtvgsvg
      301 gglgagagng gtgtvgaqgq amvmpgfplr aaavphyspy spsrfhidkr cqhrcswkcf
      361 sialillava ltamlayfat vssmrpidst kcvvvqdvka vthenahihd pistqiptee
      421 slptstaehs sasdsagdqq sdpqiqqsaq qwpavlelqa ynvahsavip pyhfwntefr
      481 nkqpafirln ltlpwganfa vygrrnvaps vtqydfvefv kggrvdhrlk rdtsseavsf
      541 mktaaqetht nvenrsyqhv likrtivepm mvnvsllqyl dtgrwflsvy ndelqpykvt
      601 lvvteaegvs ttcpndcsgr gscylgkcdc idgfqgadcs ksvcpvlcss hgqygggmch
      661 cedgwkgaec diplgdcqvp dcnqhgqcvr gscvcnpgwk gvfcdepdcs dpncsghgac
      721 vsgkcyckag wqgercnqvd qqvyqclprc sdhgtydles atciceghwt gvdcsqpscg
      781 ldcgphgtce qgvckcnddw tgtkcdqkpc dprcaehgqc kngicvcsqg wngrhctlpg
      841 cengcsrhgl ctlqdgeysc ecstgwagrd csirlemecn dfidndqdgm mdcsdseccs
      901 haacadhimc ltsnnpvdvl lrkqppsvta sfyqrvkflv eensvqsyah mneytesrva
      961 vmrgqvvteq gigivgirvs vdrdprfgft ltradgwfdv lvngggavtl qfqrspfkpl
     1021 tttvfvpwnq ivvlppvvmt lneegeshks nqppspavgf pgismglfsd hgpclehdhe
     1081 llrpiivstw mpekvgdlpg kslvfaesqi vqesiaipgs nlhlmyqssq aagylstvrm
     1141 qltgsfipks lthvhvhvei egslhtktye adpnlthtfa wnkrnvykqk vygvaqaris
     1201 igyqhstcps viwetqtatl qgfdvdisdi ggwgldihhh ynfhegilqk gdgttlhfkq
     1261 yprtvkvvmg tglqrslvcq dcdglakdar lltpvaltsg pdgsiyvgdf nlvrritpeg
     1321 nvytvltlsa tqvayqyylc vspadghlyi sdpekhqilk alslknvqhp slniepvvgs
     1381 gercipgdes hcgdegpair aklahpkgia iaadktmyia dgrniravdp ngvihtlvgh
     1441 hghhnhwspa pcvgaipahq aqlqwptglt lspldgslhf iddrlvlklt sdlkvrvvag
     1501 tplhctsngn gndsdltkvn ssiatipkkp devlgsvlav sfsptgelyi aesdshrvns
     1561 ikivdssgkm shfagqqqer lrgqcecnnt tpstkelesc actddtsste tllssnakft
     1621 aisalavspd gvlhvadqgs lhilalqpyl pnhdesgefh ipfppsnevy vfnrygqhis
     1681 tkdltsgkir ysffysknts fgklstvtds agnkiqfwrd ysgvvnsien tqdhkselki
     1741 savgflekls ergraeiala ydgstgllts rsgggetyiy nydslgrvtd vilptgeklk
     1801 lssdlsddeg llvkvsaplq alskgdkqrt vefrmkners kvltitdgtk ikkaiaftns
     1861 slevllpggg rvlsaattkh plleaalpve aemlpmwsyq lmslgeltnt mttaynlvgd
     1921 vshfqqtlnr eiwvndtrvi gvefeqafrr etfydksrnp lltvtfdpag lpltwqpheq
     1981 gynlsitydr fnrieswkwg gsdesygydr hgqlaevtns qdgtkrytyn dfnilsnitl
     2041 asdrhfslqy dddgglrhii lpsgtkhsfs cqaslgflrv tytppgssra ylqhyshdgn
     2101 llqtvfpgdg arivyryhss gqlaevvhgd gkseirytes glpsevihse rdveykwdyq
     2161 ysagllveer idfgaktgls nakftfdyds nfrlinvqgr iggqtlppht maysartgml
     2221 eqigqfkvtk pqtnettvfd gtalfsritd drfletqvtl tihqlevfrm efthdsrgri
     2281 nqtrtytrnv avntytnvkn ytwdcdgqlt gveaqepwgf kydangnmls ltyrgntipm
     2341 eynnmdrivk fgeglykydn rglvvqnare erfsynakgl lvraakrgrf dvryyydhld
     2401 rlatrkdnyg nvtqffynnq krphevsqiy sprdgklmsl vyddrghliy aqvyrhkyyi
     2461 atdqcgtpim ifsqygegir eimrspyghi vydsnpylyl pvdfcgglld qvtslvhmpn
     2521 gkvydpligk wmsplwenvl drvntpthlh lyrfngndpi dvrhtdrpnq ptdhiswlsh
     2581 lgydlkslap qlfpdelpds lvppslgpgt sifgkkkktr nlgvqsgfla hiaqrhsgda
     2641 vslsapprsa lkkdtsdlip srlgaasdpp fgkgilvsrt mdgqavvssv ptanaiygdv
     2701 ftsvfnrsyf lpftfvvhsa qqdafyfvke etwrasedrg qlkrlggqvn ttfhesengs
     2761 gssslidvki hgasyvvnlr ygttadkekq rllhhakata irkawhrere alkantptti
     2821 ewmvteqdei lksgsasnye geyihdaqly pelaedpyni rfvkkavdps skkrrrrrgv
     2881 htcklwwldk ic
//
LOCUS       XP_076546721            2654 aa            linear   INV 03-SEP-2025
DEFINITION  teneurin-a transmembrane protein isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546721
VERSION     XP_076546721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690606.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2654
                     /product="teneurin-a transmembrane protein isoform X5"
                     /calculated_mol_wt=294255
     Region          369..392
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          414..547
                     /region_name="C_rich_MXAN6577"
                     /note="MXAN_6577-like cysteine-rich domain; NF041328"
                     /db_xref="CDD:469225"
     Region          549..592
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     Region          631..657
                     /region_name="acid_disulf_rpt"
                     /note="acidic double-disulfide repeat; NF033662"
                     /db_xref="CDD:411265"
     Region          1045..1402
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          1098..1144
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1168..1210
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1225..1264
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1298..1331
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1365..2328
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          2549..2626
                     /region_name="Tox-GHH"
                     /note="GHH signature containing HNH/Endo VII superfamily
                     nuclease toxin; pfam15636"
                     /db_xref="CDD:464783"
     CDS             1..2654
                     /gene="Ten-a"
                     /coded_by="XM_076690606.1:937..8901"
                     /db_xref="GeneID:117611646"
ORIGIN      
        1 mhhyypqedi drveylmsgn prsyparspg vastptvtrl pppqqqvtvg svggglgaga
       61 gnggtgtvga qgqamvmpgf plraaavphy spyspsrfhi dkrcqhrcsw kcfsialill
      121 avaltamlay fatvssmrpi dstkcvvvqd vkavthenah ihdpistqip teeslptsta
      181 ehssasdsag dqqsdpqiqq saqqwpavle lqaynvahsa vippyhfwnt efrnkqpafi
      241 rlnltlpwga nfavygrrnv apsvtqydfv efvkggrvdh rlkrdtssea vsfmktaaqe
      301 thtnvenrsy qhvlikrtiv epmmvnvsll qyldtgrwfl svyndelqpy kvtlvvteae
      361 gvsttcpndc sgrgscylgk cdcidgfqga dcsksvcpvl csshgqyggg mchcedgwkg
      421 aecdiplgdc qvpdcnqhgq cvrgscvcnp gwkgvfcdep dcsdpncsgh gacvsgkcyc
      481 kagwqgercn qvdqqvyqcl prcsdhgtyd lesatciceg hwtgvdcsqp scgldcgphg
      541 tceqgvckcn ddwtgtkcdq kpcdprcaeh gqckngicvc sqgwngrhct lpgcengcsr
      601 hglctlqdge yscecstgwa grdcsirlem ecndfidndq dgmmdcsdse ccshaacadh
      661 imcltsnnpv dvllrkqpps vtasfyqrvk flveensvqs yahmneytes tfwssftprr
      721 vavmrgqvvt eqgigivgir vsvdrdprfg ftltradgwf dvlvngggav tlqfqrspfk
      781 pltttvfvpw nqivvlppvv mtlneegesh ksnqppspav gfpgismglf sdhgpclehd
      841 hellrpiivs twmpekvgdl pgkslvfaes qivqesiaip gsnlhlmyqs sqaagylstv
      901 rmqltgsfip kslthvhvhv eiegslhtkt yeadpnltht fawnkrnvyk qkvygvaqar
      961 isigyqhstc psviwetqta tlqgfdvdis diggwgldih hhynfhegil qkgdgttlhf
     1021 kqyprtvkvv mgtglqrslv cqdcdglakd arlltpvalt sgpdgsiyvg dfnlvrritp
     1081 egnvytvltl satqvayqyy lcvspadghl yisdpekhqi lkalslknvq hpslniepvv
     1141 gsgercipgd eshcgdegpa iraklahpkg iaiaadktmy iadgrnirav dpngvihtlv
     1201 ghhghhnhws papcvgaipa hqaqlqwptg ltlspldgsl hfiddrlvlk ltsdlkvrvv
     1261 agtplhctsn gngndsdltk vnssiatipk kpdevlgsvl avsfsptgel yiaesdshrv
     1321 nsikivdssg kmshfagqqq erlrgqcecn nttpstkele scactddtss tetllssnak
     1381 ftaisalavs pdgvlhvadq gslhilalqp ylpnhdesge fhipfppsne vyvfnrygqh
     1441 istkdltsgk irysffyskn tsfgklstvt dsagnkiqfw rdysgvvnsi entqdhksel
     1501 kisavgflek lsergraeia laydgstgll tsrsgggety iynydslgrv tdvilptgek
     1561 lklssdlsdd egllvkvsap lqalskgdkq rtvefrmkne rskvltitdg tkikkaiaft
     1621 nsslevllpg ggrvlsaatt khplleaalp veaemlpmws yqlmslgelt ntmttaynlv
     1681 gdvshfqqtl nreiwvndtr vigvefeqaf rretfydksr nplltvtfdp aglpltwqph
     1741 eqgynlsity drfnrieswk wggsdesygy drhgqlaevt nsqdgtkryt yndfnilsni
     1801 tlasdrhfsl qydddgglrh iilpsgtkhs fscqaslgfl rvtytppgss raylqhyshd
     1861 gnllqtvfpg dgarivyryh ssgqlaevvh gdgkseiryt esglpsevih serdveykwd
     1921 yqysagllve eridfgaktg lsnakftfdy dsnfrlinvq griggqtlpp htmaysartg
     1981 mleqigqfkv tkpqtnettv fdgtalfsri tddrfletqv tltihqlevf rmefthdsrg
     2041 rinqtrtytr nvavntytnv knytwdcdgq ltgveaqepw gfkydangnm lsltyrgnti
     2101 pmeynnmdri vkfgeglyky dnrglvvqna reerfsynak gllvraakrg rfdvryyydh
     2161 ldrlatrkdn ygnvtqffyn nqkrphevsq iysprdgklm slvyddrghl iyaqvyrhky
     2221 yiatdqcgtp imifsqygeg ireimrspyg hivydsnpyl ylpvdfcggl ldqvtslvhm
     2281 pngkvydpli gkwmsplwen vldrvntpth lhlyrfngnd pidvrhtdrp nqptdhiswl
     2341 shlgydlksl apqlfpdelp dslvppslgp gtsifgkkkk trnlgvqsgf lahiaqrhsg
     2401 davslsappr salkkdtsdl ipsrlgaasd ppfgkgilvs rtmdgqavvs svptanaiyg
     2461 dvftsvfnrs yflpftfvvh saqqdafyfv keetwrased rgqlkrlggq vnttfhesen
     2521 gsgssslidv kihgasyvvn lrygttadke kqrllhhaka tairkawhre realkantpt
     2581 tiewmvteqd eilksgsasn yegeyihdaq lypelaedpy nirfvkkavd psskkrrrrr
     2641 gvhtcklwwl dkic
//
LOCUS       XP_076546722            2634 aa            linear   INV 03-SEP-2025
DEFINITION  teneurin-a transmembrane protein isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546722
VERSION     XP_076546722.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690607.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2634
                     /product="teneurin-a transmembrane protein isoform X6"
                     /calculated_mol_wt=292073
     Region          349..372
                     /region_name="EGF_2"
                     /note="EGF-like domain; pfam07974"
                     /db_xref="CDD:400365"
     Region          394..527
                     /region_name="C_rich_MXAN6577"
                     /note="MXAN_6577-like cysteine-rich domain; NF041328"
                     /db_xref="CDD:469225"
     Region          529..572
                     /region_name="DSL"
                     /note="Delta serrate ligand; cl19567"
                     /db_xref="CDD:473190"
     Region          611..637
                     /region_name="acid_disulf_rpt"
                     /note="acidic double-disulfide repeat; NF033662"
                     /db_xref="CDD:411265"
     Region          1025..1382
                     /region_name="NHL"
                     /note="NHL repeat unit of beta-propeller proteins;
                     cl18310"
                     /db_xref="CDD:302697"
     Region          1078..1124
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1148..1190
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1205..1244
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1278..1311
                     /region_name="NHL repeat"
                     /note="NHL repeat [structural motif]"
                     /db_xref="CDD:271320"
     Region          1345..2308
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          2529..2606
                     /region_name="Tox-GHH"
                     /note="GHH signature containing HNH/Endo VII superfamily
                     nuclease toxin; pfam15636"
                     /db_xref="CDD:464783"
     CDS             1..2634
                     /gene="Ten-a"
                     /coded_by="XM_076690607.1:1655..9559"
                     /db_xref="GeneID:117611646"
ORIGIN      
        1 mmenfhspvp pplprrhvrl pppqqqvtvg svggglgaga gnggtgtvga qgqamvmpgf
       61 plraaavphy spyspsrfhi dkrcqhrcsw kcfsialill avaltamlay fatvssmrpi
      121 dstkcvvvqd vkavthenah ihdpistqip teeslptsta ehssasdsag dqqsdpqiqq
      181 saqqwpavle lqaynvahsa vippyhfwnt efrnkqpafi rlnltlpwga nfavygrrnv
      241 apsvtqydfv efvkggrvdh rlkrdtssea vsfmktaaqe thtnvenrsy qhvlikrtiv
      301 epmmvnvsll qyldtgrwfl svyndelqpy kvtlvvteae gvsttcpndc sgrgscylgk
      361 cdcidgfqga dcsksvcpvl csshgqyggg mchcedgwkg aecdiplgdc qvpdcnqhgq
      421 cvrgscvcnp gwkgvfcdep dcsdpncsgh gacvsgkcyc kagwqgercn qvdqqvyqcl
      481 prcsdhgtyd lesatciceg hwtgvdcsqp scgldcgphg tceqgvckcn ddwtgtkcdq
      541 kpcdprcaeh gqckngicvc sqgwngrhct lpgcengcsr hglctlqdge yscecstgwa
      601 grdcsirlem ecndfidndq dgmmdcsdse ccshaacadh imcltsnnpv dvllrkqpps
      661 vtasfyqrvk flveensvqs yahmneytes tfwssftprr vavmrgqvvt eqgigivgir
      721 vsvdrdprfg ftltradgwf dvlvngggav tlqfqrspfk pltttvfvpw nqivvlppvv
      781 mtlneegesh ksnqppspav gfpgismglf sdhgpclehd hellrpiivs twmpekvgdl
      841 pgkslvfaes qivqesiaip gsnlhlmyqs sqaagylstv rmqltgsfip kslthvhvhv
      901 eiegslhtkt yeadpnltht fawnkrnvyk qkvygvaqar isigyqhstc psviwetqta
      961 tlqgfdvdis diggwgldih hhynfhegil qkgdgttlhf kqyprtvkvv mgtglqrslv
     1021 cqdcdglakd arlltpvalt sgpdgsiyvg dfnlvrritp egnvytvltl satqvayqyy
     1081 lcvspadghl yisdpekhqi lkalslknvq hpslniepvv gsgercipgd eshcgdegpa
     1141 iraklahpkg iaiaadktmy iadgrnirav dpngvihtlv ghhghhnhws papcvgaipa
     1201 hqaqlqwptg ltlspldgsl hfiddrlvlk ltsdlkvrvv agtplhctsn gngndsdltk
     1261 vnssiatipk kpdevlgsvl avsfsptgel yiaesdshrv nsikivdssg kmshfagqqq
     1321 erlrgqcecn nttpstkele scactddtss tetllssnak ftaisalavs pdgvlhvadq
     1381 gslhilalqp ylpnhdesge fhipfppsne vyvfnrygqh istkdltsgk irysffyskn
     1441 tsfgklstvt dsagnkiqfw rdysgvvnsi entqdhksel kisavgflek lsergraeia
     1501 laydgstgll tsrsgggety iynydslgrv tdvilptgek lklssdlsdd egllvkvsap
     1561 lqalskgdkq rtvefrmkne rskvltitdg tkikkaiaft nsslevllpg ggrvlsaatt
     1621 khplleaalp veaemlpmws yqlmslgelt ntmttaynlv gdvshfqqtl nreiwvndtr
     1681 vigvefeqaf rretfydksr nplltvtfdp aglpltwqph eqgynlsity drfnrieswk
     1741 wggsdesygy drhgqlaevt nsqdgtkryt yndfnilsni tlasdrhfsl qydddgglrh
     1801 iilpsgtkhs fscqaslgfl rvtytppgss raylqhyshd gnllqtvfpg dgarivyryh
     1861 ssgqlaevvh gdgkseiryt esglpsevih serdveykwd yqysagllve eridfgaktg
     1921 lsnakftfdy dsnfrlinvq griggqtlpp htmaysartg mleqigqfkv tkpqtnettv
     1981 fdgtalfsri tddrfletqv tltihqlevf rmefthdsrg rinqtrtytr nvavntytnv
     2041 knytwdcdgq ltgveaqepw gfkydangnm lsltyrgnti pmeynnmdri vkfgeglyky
     2101 dnrglvvqna reerfsynak gllvraakrg rfdvryyydh ldrlatrkdn ygnvtqffyn
     2161 nqkrphevsq iysprdgklm slvyddrghl iyaqvyrhky yiatdqcgtp imifsqygeg
     2221 ireimrspyg hivydsnpyl ylpvdfcggl ldqvtslvhm pngkvydpli gkwmsplwen
     2281 vldrvntpth lhlyrfngnd pidvrhtdrp nqptdhiswl shlgydlksl apqlfpdelp
     2341 dslvppslgp gtsifgkkkk trnlgvqsgf lahiaqrhsg davslsappr salkkdtsdl
     2401 ipsrlgaasd ppfgkgilvs rtmdgqavvs svptanaiyg dvftsvfnrs yflpftfvvh
     2461 saqqdafyfv keetwrased rgqlkrlggq vnttfhesen gsgssslidv kihgasyvvn
     2521 lrygttadke kqrllhhaka tairkawhre realkantpt tiewmvteqd eilksgsasn
     2581 yegeyihdaq lypelaedpy nirfvkkavd psskkrrrrr gvhtcklwwl dkic
//
LOCUS       XP_034195568            1239 aa            linear   INV 03-SEP-2025
DEFINITION  dosage compensation regulator mle isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195568
VERSION     XP_034195568.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339677.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195568.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1239
                     /product="dosage compensation regulator mle isoform X1"
                     /calculated_mol_wt=138779
     Region          3..71
                     /region_name="DSRM_DHX9_rpt1"
                     /note="first double-stranded RNA binding motif of DEAH box
                     protein 9 (DHX9) and similar proteins; cd19854"
                     /db_xref="CDD:380683"
     Site            order(5..6,9..10,13,16,34,53..55,58)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380683"
     Region          162..236
                     /region_name="DSRM_DHX9_rpt2"
                     /note="second double-stranded RNA binding motif of DEAH
                     box protein 9 (DHX9) and similar proteins; cd19855"
                     /db_xref="CDD:380684"
     Site            order(163,166..167,170..171,174..175,220,224)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:380684"
     Region          325..558
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Region          381..>930
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     Site            order(408..414,506)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     Region          993..1072
                     /region_name="OB_NTP_bind"
                     /note="Oligonucleotide/oligosaccharide-binding (OB)-fold;
                     pfam07717"
                     /db_xref="CDD:400182"
     CDS             1..1239
                     /gene="mle"
                     /coded_by="XM_034339677.2:351..4070"
                     /db_xref="GeneID:117611647"
ORIGIN      
        1 mgdiksflhq wctkngkepq fdvrptglkh rqrflcelri pgfdyvgagn stnkkdaqgn
       61 aardyvnylv rtghvnandv pkdtnnlqpf dtvpikvevq npttksvfqd gmgpndigha
      121 yrvynehgqg nytymdriad qkrvedaedv dvnsgihgnw tienaksklh qfmqtnkint
      181 dykytqlgpd htrsfmaemt fyvkqlgrdi tgretgsnkq taskscalsl vrqlyhlgvi
      241 eafngtlkkn keaeqmkpyp vkispelidq icdvltslnv tpisiksvee svqqqedqst
      301 navslltnqv lddfissvpq pagvvswspp qqnwdpwtgc nidegplaam tleqlsedll
      361 yeqrerlqqd vnlqqiiker stlpvfsmkn eimnainens iiiiqgntgc gkttqvcqfi
      421 lddyiasgqg afcsiaitqp rrisavsvad rvatergenl gesvgysvrf esalprpygs
      481 imfctvgvll rklegglrgv shvivdeihe rdvnsdfimv vlrdmihmyp dlriilmsat
      541 idttlfsnyf nncpiievpg rsypvqryfl edciqltnfv ppmdskkrks rdtddlpteg
      601 epeenlnkvi getysiqtkn amaqltekei sfelieallr yikeqgipga vliflpgwnl
      661 ifalmkhlqq hpvyggpdyv iiplhsqlpr edqrkvfdpv dvgitkiila tniaetsiti
      721 ndvvyvidsc kakmklftsh nnmtnyatvw asrtnleqre gragrvkpgf cfhlcskarf
      781 nkmdehmtpe mfrtplhela lsikllrlgs igkflskaie pppidaviea evilremkcl
      841 dkndeltplg kilarlpiep rlgkmmilgc mfrvgdalst maansttfpe vynmgpdvrr
      901 lsaqqkwfag arysdhvaml hafqaweear lggeyaeqtf cdsknlsmpt lritweaknq
      961 lqallqnagf pqetlcptpl nyqagadvrl dtitallcmg lypnvcyhke krkvlttesk
     1021 aalihktsvn csnfeqnfpf pffvfgekir travsckqmt mvspihlllf gsrkveytng
     1081 vvkldnwinl dmdpnhaaai valrpalesl vvkaakepet ilelspneek vlnvikslcg
     1141 mnscrynldq itgggfqsrr pprghstnfy sdngnpakmm rmgggfrgsp rgggegsgys
     1201 rnsyfggyrg ggnrgygmqn srgwgggnwg grgnfrgrf
//
LOCUS       XP_034195569            1047 aa            linear   INV 03-SEP-2025
DEFINITION  dosage compensation regulator mle isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195569
VERSION     XP_034195569.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339678.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195569.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1047
                     /product="dosage compensation regulator mle isoform X2"
                     /calculated_mol_wt=116927
     Region          <2..44
                     /region_name="DSRM_SF"
                     /note="double-stranded RNA binding motif (DSRM)
                     superfamily; cl00054"
                     /db_xref="CDD:444671"
     Region          133..366
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     Region          189..>738
                     /region_name="HrpA"
                     /note="HrpA-like RNA helicase [Translation, ribosomal
                     structure and biogenesis]; COG1643"
                     /db_xref="CDD:441249"
     Site            order(216..222,314)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:350670"
     Region          801..880
                     /region_name="OB_NTP_bind"
                     /note="Oligonucleotide/oligosaccharide-binding (OB)-fold;
                     pfam07717"
                     /db_xref="CDD:400182"
     CDS             1..1047
                     /gene="mle"
                     /coded_by="XM_034339678.2:243..3386"
                     /db_xref="GeneID:117611647"
ORIGIN      
        1 msfmaemtfy vkqlgrditg retgsnkqta skscalslvr qlyhlgviea fngtlkknke
       61 aeqmkpypvk ispelidqic dvltslnvtp isiksveesv qqqedqstna vslltnqvld
      121 dfissvpqpa gvvswsppqq nwdpwtgcni degplaamtl eqlsedllye qrerlqqdvn
      181 lqqiikerst lpvfsmknei mnainensii iiqgntgcgk ttqvcqfild dyiasgqgaf
      241 csiaitqprr isavsvadrv atergenlge svgysvrfes alprpygsim fctvgvllrk
      301 legglrgvsh vivdeiherd vnsdfimvvl rdmihmypdl riilmsatid ttlfsnyfnn
      361 cpiievpgrs ypvqryfled ciqltnfvpp mdskkrksrd tddlptegep eenlnkvige
      421 tysiqtknam aqltekeisf elieallryi keqgipgavl iflpgwnlif almkhlqqhp
      481 vyggpdyvii plhsqlpred qrkvfdpvdv gitkiilatn iaetsitind vvyvidscka
      541 kmklftshnn mtnyatvwas rtnleqregr agrvkpgfcf hlcskarfnk mdehmtpemf
      601 rtplhelals ikllrlgsig kflskaiepp pidavieaev ilremkcldk ndeltplgki
      661 larlpieprl gkmmilgcmf rvgdalstma ansttfpevy nmgpdvrrls aqqkwfagar
      721 ysdhvamlha fqaweearlg geyaeqtfcd sknlsmptlr itweaknqlq allqnagfpq
      781 etlcptplny qagadvrldt itallcmgly pnvcyhkekr kvltteskaa lihktsvncs
      841 nfeqnfpfpf fvfgekirtr avsckqmtmv spihlllfgs rkveytngvv kldnwinldm
      901 dpnhaaaiva lrpaleslvv kaakepetil elspneekvl nvikslcgmn scrynldqit
      961 gggfqsrrpp rghstnfysd ngnpakmmrm gggfrgsprg ggegsgysrn syfggyrggg
     1021 nrgygmqnsr gwgggnwggr gnfrgrf
//
LOCUS       XP_034195570             281 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin-11 [Osmia lignaria lignaria].
ACCESSION   XP_034195570
VERSION     XP_034195570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339679.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..281
                     /product="tetraspanin-11"
                     /calculated_mol_wt=32082
     Region          66..>201
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          163..251
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cd03127"
                     /db_xref="CDD:239401"
     Site            order(165,169,171..172,175,189,192..193,196..197)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239401"
     CDS             1..281
                     /gene="LOC117611648"
                     /coded_by="XM_034339679.2:186..1031"
                     /db_xref="GeneID:117611648"
ORIGIN      
        1 mthmnwnkff tgtkdndhrn dkdetesede editdsnata stdkkpyflg igfhkfssnr
       61 lskrcikfvf ivlnsamfla givgsvisiw tltdnlimsr ligqrlfvtt lllisliasf
      121 vslfgifgll kkrrnflniy awcyllflcv ifisaimsfw ifeeiirrih admvssirry
      181 dslpsskeaw dnthrylkcc ginssndwak ysidipkscc srnieeclqm tldvsyksgc
      241 lknavlllks hvhaisistv llfliallnf llamcilarm n
//
LOCUS       XP_034195902            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195902
VERSION     XP_034195902.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340011.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195902.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X1"
                     /calculated_mol_wt=344674
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_034340011.2:2330..11593"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_076546723            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546723
VERSION     XP_076546723.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690608.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X1"
                     /calculated_mol_wt=344674
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_076690608.1:1883..11146"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_076546724            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546724
VERSION     XP_076546724.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690609.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X1"
                     /calculated_mol_wt=344674
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_076690609.1:1970..11233"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_076546725            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546725
VERSION     XP_076546725.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690610.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X1"
                     /calculated_mol_wt=344674
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_076690610.1:1935..11198"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_076546726            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546726
VERSION     XP_076546726.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690611.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X1"
                     /calculated_mol_wt=344674
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_076690611.1:1848..11111"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_076546727            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546727
VERSION     XP_076546727.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690612.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X1"
                     /calculated_mol_wt=344674
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_076690612.1:12108..21371"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_034195903            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195903
VERSION     XP_034195903.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340012.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195903.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X2"
                     /calculated_mol_wt=344629
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_034340012.2:2330..11593"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirsvf
     1981 gvdeeahtrn mravkqsvld gtskwsakia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_076546728            3087 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546728
VERSION     XP_076546728.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690613.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3087
                     /product="unc-13 isoform X1"
                     /calculated_mol_wt=344674
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2930..3051
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3087
                     /gene="unc-13"
                     /coded_by="XM_076690613.1:11945..21208"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp lnkasqlrqr qrsmssergg degegaegme
     2881 sleeplrqsk pslrresrqv pdtagtdegs vgevsiqvdl fthpgtgeqk vtvkvvaand
     2941 lkwqlatgmf rpyvevnlig phltgkkrkq stksksnnws pqfnesfdfm igneqqqldf
     3001 yelhicvkdy cfaredrlvg vavmqlkdiv eegscacwls lgkriqmdet gwtilrilsq
     3061 rnndeiakef vklksdirqe tplpnpt
//
LOCUS       XP_076546729            3086 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546729
VERSION     XP_076546729.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690614.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3086
                     /product="unc-13 isoform X3"
                     /calculated_mol_wt=344545
     Region          1865..1946
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2006..2132
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2022,2028,2074,2076,2093)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2323..2840
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2929..3050
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3086
                     /gene="unc-13"
                     /coded_by="XM_076690614.1:2330..11590"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelmhvykkt lqamiypiss ttphkfvtwt atsptycyec egllwgiarq gvrctecgvk
     1921 chekckdlln adclqraaek sskhgaedka nsiitamker mkqrerekpe ifelirttft
     1981 vdpdthidsl eqaeqivleg tskwsckiai tvicaqglia kdksgtsdpy vtvqvgkvkk
     2041 rtrtmpreln pvwhekfyfe chnssdrikv rvwdedndlk sklrqkltre sddflgqtii
     2101 evrtlsgemd vwynlekrtd ksavsgairl hisveikgee kvapyhvqyt clhenlfhsl
     2161 cekndgvval pqakgddawk vyfdppgeel vdefamrygi esiyqamthf hclstkylcp
     2221 gvpavmstll aninayyaht sassavsasd rfaasnfgke kfvklldqlh nslridlsmy
     2281 rnnfpassqe klmdlkstvd lltsitffrm kvqelssppr astvvkdcvk aclrstyqfl
     2341 fencydiynr efqvdpseak rdaedhgpsl esldfwhkli alivsvieed knsyapvlnq
     2401 fpqelnigql saatmwslfa vdmkyaleeh eqhrlckssa ymnlhfkvkw lhtnyvkdvp
     2461 pykgavpeyp awfepfvmqw lnenddvsle ylhgafnrdk kdgfqksseh alfsvsvvdv
     2521 ftqltqcfdv vsklecpdpe iwkrymkrfa ktivkvlvay adivkkefps hlkeeriaci
     2581 lmnniqqlrv qlekmfesmg gekleedaan ilkelqqqln galddlamlf aksleprita
     2641 svkelgdlll aikgggqvql sqpaqrnnva veadevlrpl mvlldgslsl yaescektvl
     2701 krllkelwki vmrilektvv lppmsdksmm fknltdnakn laanakiedm sklfknhmsg
     2761 kpdvknalsg vmdiskevek nlspkqcavl evaldtikqy fhaggnglkk aflekspelq
     2821 slryalslyt qttdtliktf vtsqvnqvpl nkasqlrqrq rsmsserggd egegaegmes
     2881 leeplrqskp slrresrqvp dtagtdegsv gevsiqvdlf thpgtgeqkv tvkvvaandl
     2941 kwqlatgmfr pyvevnligp hltgkkrkqs tksksnnwsp qfnesfdfmi gneqqqldfy
     3001 elhicvkdyc faredrlvgv avmqlkdive egscacwlsl gkriqmdetg wtilrilsqr
     3061 nndeiakefv klksdirqet plpnpt
//
LOCUS       XP_076546730            3036 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546730
VERSION     XP_076546730.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690615.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3036
                     /product="unc-13 isoform X4"
                     /calculated_mol_wt=338922
     Region          1866..1947
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          2007..2133
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(2023,2029,2075,2077,2094)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          2324..2841
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2879..3000
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..3036
                     /gene="unc-13"
                     /coded_by="XM_076690615.1:2329..11439"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 mkmdstklgg paptsprads palptyatta dgnidyiigd ymerlgtrln iletelkyaw
       61 raldllsqey ikmwerlekl egllyeqqtv isqliefyts ggthenanva eesmasqqss
      121 vseldaiaks igatmgieet talreklaqq emarmhgltq dsttaaavvt dmhrnvrnld
      181 tletlaeesn ipdeafyrsl nnayredlic gdtsrptsql gmiweeaede dvngkkeidt
      241 tmfektatkd teleelagkp tkeeiqesvt kkedeadede gevfsaadyk syrgntpcvs
      301 eqdlaqlsrl ssidqvalek lheldrltnk lqkdsqnlie lqsrlmdspk rqynseaeak
      361 laeeasside qlrkiyaetd vdswtfsksp gstgrsisrv stdsglatdg dfttrsispr
      421 ltstsrtnld sisstytppr lgyaatvrek spdpmiqlpi dvgsfakgya enkeltdlmv
      481 esfaavtcas ptiystttdk vpsptlsags vhsvhsvhsv qslrtrqdgy fgsaprlnle
      541 fqesrtppsp spssppppap qdsgetflmp gtdqrepdtk rsqsptflrs teklvsleqg
      601 rlsprtphsp ksprvspkht akpgsvaniv aaksdsglss msgwssldks psspkssisk
      661 mltsyaveha raslistttt grssspippl petttsvvtq eplydtpegr dafaataats
      721 hsyaaamnhl saftsmktpt tkddynfvpp paptattcqs pkkrsrqqtl qqhtydtvap
      781 gstmdyvyrs eqpaiysvag snrqqaitsv ytsgsgtygp kttttayypd smdqysyqen
      841 fssaqytess gmahakkvlr saayadgmtn hegykaamyr tmfptgnitd alsyyptsan
      901 lprtetness wlnsmedqip hdstsssirs ltsdgsysqs pytdrrypqp payqahqyhh
      961 tyanqgypqa yqqqyqpynq rlsssesaql adgyqrqwqr ehqyiqdheg grlpidqaqh
     1021 qqseyydass vivsqsgyis iatnlkdhem enakagkkir rgsslknams smsnwwpdlh
     1081 lskrhrshsl pggvrredld msqepqqklp adagvtrgrg rgqtarkkkk hilvstvsgi
     1141 lqkakrrthq sqslsdpeqs etewssgrqs glsededsvm sdanqeppff akvktastkr
     1201 kpvsqpvvqq qqpttqplpl qqkeyiqqqq qqqnlqqqqq vlqqqiqqhq eqlqqqalqd
     1261 gwgkekdqrk eldhdgsdqm ggfdeetsgk stgsgsggss ifqtvgdikr stgssdeapn
     1321 ekapklppvt lmgasmefav sralgkyrqr qsstvsdeqp pseeassekk sedgtvqtle
     1381 tsfeypedvs ekseksekss strlpelvts iseegppseg spsasstrgq ttgtrflprh
     1441 qqsleipwtg srpgeldedn rsthsyrsts rvssrrqste dsidsedewy ryelrkleel
     1501 erqsqievem gevlveepee tehyqpddtv kekmsfvlke lklkakpreq ikeekeevrg
     1561 skingtlakd rrieerdryr dekpipkrks aesieavfar vtdyhtwahe eeakkekpss
     1621 ameegssget sgpdspvqsm deeeeeelpk dvdeeqsrrs ssgstfrqge kihpgsepis
     1681 regsvsvpps evsvsipgaw dsestvlegl erdgagsaet attatlslpk ikidssscgs
     1741 sdttlkdgqv spgppgskwk llkalkerka eekmkeveea skeptisqgp tangsgpgge
     1801 sggrgnghlg dnpfysnids mpdirprrks vplvseltma atkrntagla savpratlnd
     1861 eelkmhvykk tlqamiypis sttphkfvtw tatsptycye cegllwgiar qgvrctecgv
     1921 kchekckdll nadclqraae ksskhgaedk ansiitamke rmkqrerekp eifelirttf
     1981 tvdpdthids leqaeqivle gtskwsckia itvicaqgli akdksgtsdp yvtvqvgkvk
     2041 krtrtmprel npvwhekfyf echnssdrik vrvwdedndl ksklrqkltr esddflgqti
     2101 ievrtlsgem dvwynlekrt dksavsgair lhisveikge ekvapyhvqy tclhenlfhs
     2161 lcekndgvva lpqakgddaw kvyfdppgee lvdefamryg iesiyqamth fhclstkylc
     2221 pgvpavmstl laninayyah tsassavsas drfaasnfgk ekfvklldql hnslridlsm
     2281 yrnnfpassq eklmdlkstv dlltsitffr mkvqelsspp rastvvkdcv kaclrstyqf
     2341 lfencydiyn refqvdpsea krdaedhgps lesldfwhkl ialivsviee dknsyapvln
     2401 qfpqelnigq lsaatmwslf avdmkyalee heqhrlckss aymnlhfkvk wlhtnyvkdv
     2461 ppykgavpey pawfepfvmq wlnenddvsl eylhgafnrd kkdgfqksse halfsvsvvd
     2521 vftqltqcfd vvsklecpdp eiwkrymkrf aktivkvlva yadivkkefp shlkeeriac
     2581 ilmnniqqlr vqlekmfesm ggekleedaa nilkelqqql ngalddlaml faksleprit
     2641 asvkelgdll laikgggqvq lsqpaqrnnv aveadevlrp lmvlldgsls lyaescektv
     2701 lkrllkelwk ivmrilektv vlppmsdksm mfknltdnak nlaanakied msklfknhms
     2761 gkpdvknals gvmdiskeve knlspkqcav levaldtikq yfhaggnglk kaflekspel
     2821 qslryalsly tqttdtlikt fvtsqvnqvp dtagtdegsv gevsiqvdlf thpgtgeqkv
     2881 tvkvvaandl kwqlatgmfr pyvevnligp hltgkkrkqs tksksnnwsp qfnesfdfmi
     2941 gneqqqldfy elhicvkdyc faredrlvgv avmqlkdive egscacwlsl gkriqmdetg
     3001 wtilrilsqr nndeiakefv klksdirqet plpnpt
//
LOCUS       XP_076546731            2723 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546731
VERSION     XP_076546731.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690616.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2723
                     /product="unc-13 isoform X5"
                     /calculated_mol_wt=311628
     Region          1..187
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          264..>849
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <866..>1106
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1058..>1406
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1502..1583
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1643..1769
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1659,1665,1711,1713,1730)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1960..2477
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2566..2687
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2723
                     /gene="unc-13"
                     /coded_by="XM_076690616.1:118..8289"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne vtskqsynts kstvstgkqe
      121 qeqqqqqhis ldsmrdtnsn tqappdftnt egsgqwvsld selemrgaei igtkkptghs
      181 llidcrlelp fgkceqrtrw kslltsetam qlraarpmcg sdpenteaad lqrklemlnn
      241 mmdqearaeq arrqilynig hsgysedsdy tsdlnypvgg qganssasqf rtaanqlatp
      301 qrsletsren syerddhqip atvggrlaps nygnyggsgh sprydepype egppqrysqs
      361 qhasessepl fynsrprhyk eyrrrkhtwd yedsqdgwys egydyhgtrl detriysdte
      421 yypstttsss rrraphrrps lerqmtlydd hsyytdgyss dgrvgkmsat ypecqtdiry
      481 neyydsitgy gyqdesrygq ddedrqwdsg gkwylgssty yenkrnrktl pqrpassigi
      541 hrpdyrptvt rqrsyeseel nysshstrrr kplqpqqrps srtegtgkkl pptpttpsnv
      601 vinrklaslp atpsrqlpkt rtpseesyyk sdynedynya yrsqdnlpqd tyidsiyseq
      661 sgydqmhvpg ktqyaedsqy gssytpqvdd hygrsyqepq tqdtydqtyl qnsykdeyqk
      721 pyveqntqvy qdtyqdmtyp nasqpndqyt sqpndqyrkt srdmiiddny qvmgfdknnv
      781 qyqdrtkvhp ykedtyqeqy edysvsvpss vydqnnvtnt ynqyqqdqyd sqynintstg
      841 yqksypdtyn qetyktevyd qdtynqgtyn qetykqdtyn qetykpdtyk edtykqdtyn
      901 qgtyiqdtyn qetynqgtyn qetypqdtyt qdtynqvpit sqsnyedays kpqkdyvdyq
      961 tpytkeetvt ttykneyeeq yqdypdqyee syhdsypgsf eerykdspsa sterrssevp
     1021 elsvttprgq trpngyqsse sdyyftsqes qdlsstglsr rkklekrdas plvqqntdsl
     1081 esrddelkes ietavssiss ipqkkvisdy stagdsspaa ptltespqst vqpsttmvnh
     1141 vtpnhvppsm tvtamvhptt tangkrltra esyqeevied eeypeiipke pqrkdsqlsh
     1201 qsqlsqhssq hsqhsqkpkl trgdsyasdh fpeesysrrs syaqptrkds fssttqesfk
     1261 ppltrtdsyq krgsygqnft apqpisraes yqrgyfkdqd sieepevgis navnddykmr
     1321 depleryera deamirgsre gslvdaykts ppmshtespr gsitkqasle esvqmdtppr
     1381 sppepppdee llemvgaapv ptqlkerpem tarqrwhway nqivmqlrng sgpggesggr
     1441 gnghlgdnpf ysnidsmpdi rprrksvplv seltmaatkr ntaglasavp ratlndeelk
     1501 mhvykktlqa miypissttp hkfvtwtats ptycyecegl lwgiarqgvr ctecgvkche
     1561 kckdllnadc lqraaekssk hgaedkansi itamkermkq rerekpeife lirttftvdp
     1621 dthidsleqa eqivlegtsk wsckiaitvi caqgliakdk sgtsdpyvtv qvgkvkkrtr
     1681 tmprelnpvw hekfyfechn ssdrikvrvw dedndlkskl rqkltresdd flgqtiievr
     1741 tlsgemdvwy nlekrtdksa vsgairlhis veikgeekva pyhvqytclh enlfhslcek
     1801 ndgvvalpqa kgddawkvyf dppgeelvde famrygiesi yqamthfhcl stkylcpgvp
     1861 avmstllani nayyahtsas savsasdrfa asnfgkekfv klldqlhnsl ridlsmyrnn
     1921 fpassqeklm dlkstvdllt sitffrmkvq elsspprast vvkdcvkacl rstyqflfen
     1981 cydiynrefq vdpseakrda edhgpslesl dfwhkliali vsvieedkns yapvlnqfpq
     2041 elnigqlsaa tmwslfavdm kyaleeheqh rlckssaymn lhfkvkwlht nyvkdvppyk
     2101 gavpeypawf epfvmqwlne nddvsleylh gafnrdkkdg fqkssehalf svsvvdvftq
     2161 ltqcfdvvsk lecpdpeiwk rymkrfakti vkvlvayadi vkkefpshlk eeriacilmn
     2221 niqqlrvqle kmfesmggek leedaanilk elqqqlngal ddlamlfaks lepritasvk
     2281 elgdlllaik gggqvqlsqp aqrnnvavea devlrplmvl ldgslslyae scektvlkrl
     2341 lkelwkivmr ilektvvlpp msdksmmfkn ltdnaknlaa nakiedmskl fknhmsgkpd
     2401 vknalsgvmd iskeveknls pkqcavleva ldtikqyfha ggnglkkafl ekspelqslr
     2461 yalslytqtt dtliktfvts qvnqvplnka sqlrqrqrsm sserggdege gaegmeslee
     2521 plrqskpslr resrqvpdta gtdegsvgev siqvdlfthp gtgeqkvtvk vvaandlkwq
     2581 latgmfrpyv evnligphlt gkkrkqstks ksnnwspqfn esfdfmigne qqqldfyelh
     2641 icvkdycfar edrlvgvavm qlkdiveegs cacwlslgkr iqmdetgwti lrilsqrnnd
     2701 eiakefvklk sdirqetplp npt
//
LOCUS       XP_076546732            2722 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076546732
VERSION     XP_076546732.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690617.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2722
                     /product="unc-13 isoform X6"
                     /calculated_mol_wt=311541
     Region          1..187
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          260..>848
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <865..>1105
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1057..>1405
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1501..1582
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1642..1768
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1658,1664,1710,1712,1729)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1959..2476
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2565..2686
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2722
                     /gene="unc-13"
                     /coded_by="XM_076690617.1:118..8286"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne vtskqsynts kstvstgkqe
      121 qeqqqqqhis ldsmrdtnsn tqappdftnt egsgqwvsld selemrgaei igtkkptghs
      181 llidcrlelp fgkceqrtrw kslltsetam qlraarpmcg sdpenteaad lqrklemlnn
      241 mmdqearaeq arrqilynig hsgysedsdy tsdlnypvgg qganssasqf rtaanqlatp
      301 qrsletsren syerddhqip atvggrlaps nygnyggsgh sprydepype egppqrysqs
      361 qhasessepl fynsrprhyk eyrrrkhtwd yedsqdgwys egydyhgtrl detriysdte
      421 yypstttsss rrraphrrps lerqmtlydd hsyytdgyss dgrvgkmsat ypecqtdiry
      481 neyydsitgy gyqderygqd dedrqwdsgg kwylgsstyy enkrnrktlp qrpassigih
      541 rpdyrptvtr qrsyeseeln ysshstrrrk plqpqqrpss rtegtgkklp ptpttpsnvv
      601 inrklaslpa tpsrqlpktr tpseesyyks dynedynyay rsqdnlpqdt yidsiyseqs
      661 gydqmhvpgk tqyaedsqyg ssytpqvddh ygrsyqepqt qdtydqtylq nsykdeyqkp
      721 yveqntqvyq dtyqdmtypn asqpndqyts qpndqyrkts rdmiiddnyq vmgfdknnvq
      781 yqdrtkvhpy kedtyqeqye dysvsvpssv ydqnnvtnty nqyqqdqyds qynintstgy
      841 qksypdtynq etyktevydq dtynqgtynq etykqdtynq etykpdtyke dtykqdtynq
      901 gtyiqdtynq etynqgtynq etypqdtytq dtynqvpits qsnyedaysk pqkdyvdyqt
      961 pytkeetvtt tykneyeeqy qdypdqyees yhdsypgsfe erykdspsas terrssevpe
     1021 lsvttprgqt rpngyqsses dyyftsqesq dlsstglsrr kklekrdasp lvqqntdsle
     1081 srddelkesi etavssissi pqkkvisdys tagdsspaap tltespqstv qpsttmvnhv
     1141 tpnhvppsmt vtamvhpttt angkrltrae syqeeviede eypeiipkep qrkdsqlshq
     1201 sqlsqhssqh sqhsqkpklt rgdsyasdhf peesysrrss yaqptrkdsf ssttqesfkp
     1261 pltrtdsyqk rgsygqnfta pqpisraesy qrgyfkdqds ieepevgisn avnddykmrd
     1321 epleryerad eamirgsreg slvdayktsp pmshtesprg sitkqaslee svqmdtpprs
     1381 ppepppdeel lemvgaapvp tqlkerpemt arqrwhwayn qivmqlrngs gpggesggrg
     1441 nghlgdnpfy snidsmpdir prrksvplvs eltmaatkrn taglasavpr atlndeelkm
     1501 hvykktlqam iypissttph kfvtwtatsp tycyecegll wgiarqgvrc tecgvkchek
     1561 ckdllnadcl qraaeksskh gaedkansii tamkermkqr erekpeifel irttftvdpd
     1621 thidsleqae qivlegtskw sckiaitvic aqgliakdks gtsdpyvtvq vgkvkkrtrt
     1681 mprelnpvwh ekfyfechns sdrikvrvwd edndlksklr qkltresddf lgqtiievrt
     1741 lsgemdvwyn lekrtdksav sgairlhisv eikgeekvap yhvqytclhe nlfhslcekn
     1801 dgvvalpqak gddawkvyfd ppgeelvdef amrygiesiy qamthfhcls tkylcpgvpa
     1861 vmstllanin ayyahtsass avsasdrfaa snfgkekfvk lldqlhnslr idlsmyrnnf
     1921 passqeklmd lkstvdllts itffrmkvqe lsspprastv vkdcvkaclr styqflfenc
     1981 ydiynrefqv dpseakrdae dhgpslesld fwhklialiv svieedknsy apvlnqfpqe
     2041 lnigqlsaat mwslfavdmk yaleeheqhr lckssaymnl hfkvkwlhtn yvkdvppykg
     2101 avpeypawfe pfvmqwlnen ddvsleylhg afnrdkkdgf qkssehalfs vsvvdvftql
     2161 tqcfdvvskl ecpdpeiwkr ymkrfaktiv kvlvayadiv kkefpshlke eriacilmnn
     2221 iqqlrvqlek mfesmggekl eedaanilke lqqqlngald dlamlfaksl epritasvke
     2281 lgdlllaikg ggqvqlsqpa qrnnvavead evlrplmvll dgslslyaes cektvlkrll
     2341 kelwkivmri lektvvlppm sdksmmfknl tdnaknlaan akiedmsklf knhmsgkpdv
     2401 knalsgvmdi skeveknlsp kqcavleval dtikqyfhag gnglkkafle kspelqslry
     2461 alslytqttd tliktfvtsq vnqvplnkas qlrqrqrsms serggdegeg aegmesleep
     2521 lrqskpslrr esrqvpdtag tdegsvgevs iqvdlfthpg tgeqkvtvkv vaandlkwql
     2581 atgmfrpyve vnligphltg kkrkqstksk snnwspqfne sfdfmigneq qqldfyelhi
     2641 cvkdycfare drlvgvavmq lkdiveegsc acwlslgkri qmdetgwtil rilsqrnnde
     2701 iakefvklks dirqetplpn pt
//
LOCUS       XP_076546733            2714 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546733
VERSION     XP_076546733.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690618.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2714
                     /product="unc-13 isoform X7"
                     /calculated_mol_wt=310665
     Region          1..178
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          255..>840
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <857..>1097
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1049..>1397
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1493..1574
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1634..1760
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1650,1656,1702,1704,1721)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1951..2468
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2557..2678
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2714
                     /gene="unc-13"
                     /coded_by="XM_076690618.1:118..8262"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceeadq fnsyvtlklq nvksttvtvk ganpcweqdf lfetndmntg
       61 llvevwckgf lwdrflgyyy vplsevsymn evtskqsynt skstvstgkq eqeqqqqqhi
      121 sldsmrdtns ntqappdftn tegsgqwvsl dselemrgae iigtkkptgh sllidcrlel
      181 pfgkceqrtr wkslltseta mqlraarpmc gsdpenteaa dlqrklemln nmmdqearae
      241 qarrqilyni ghsgysedsd ytsdlnypvg gqganssasq frtaanqlat pqrsletsre
      301 nsyerddhqi patvggrlap snygnyggsg hsprydepyp eegppqrysq sqhasessep
      361 lfynsrprhy keyrrrkhtw dyedsqdgwy segydyhgtr ldetriysdt eyypstttss
      421 srrraphrrp slerqmtlyd dhsyytdgys sdgrvgkmsa typecqtdir yneyydsitg
      481 ygyqdesryg qddedrqwds ggkwylgsst yyenkrnrkt lpqrpassig ihrpdyrptv
      541 trqrsyesee lnysshstrr rkplqpqqrp ssrtegtgkk lpptpttpsn vvinrklasl
      601 patpsrqlpk trtpseesyy ksdynedyny ayrsqdnlpq dtyidsiyse qsgydqmhvp
      661 gktqyaedsq ygssytpqvd dhygrsyqep qtqdtydqty lqnsykdeyq kpyveqntqv
      721 yqdtyqdmty pnasqpndqy tsqpndqyrk tsrdmiiddn yqvmgfdknn vqyqdrtkvh
      781 pykedtyqeq yedysvsvps svydqnnvtn tynqyqqdqy dsqynintst gyqksypdty
      841 nqetyktevy dqdtynqgty nqetykqdty nqetykpdty kedtykqdty nqgtyiqdty
      901 nqetynqgty nqetypqdty tqdtynqvpi tsqsnyeday skpqkdyvdy qtpytkeetv
      961 tttykneyee qyqdypdqye esyhdsypgs feerykdsps asterrssev pelsvttprg
     1021 qtrpngyqss esdyyftsqe sqdlsstgls rrkklekrda splvqqntds lesrddelke
     1081 sietavssis sipqkkvisd ystagdsspa aptltespqs tvqpsttmvn hvtpnhvpps
     1141 mtvtamvhpt ttangkrltr aesyqeevie deeypeiipk epqrkdsqls hqsqlsqhss
     1201 qhsqhsqkpk ltrgdsyasd hfpeesysrr ssyaqptrkd sfssttqesf kppltrtdsy
     1261 qkrgsygqnf tapqpisrae syqrgyfkdq dsieepevgi snavnddykm rdepleryer
     1321 adeamirgsr egslvdaykt sppmshtesp rgsitkqasl eesvqmdtpp rsppepppde
     1381 ellemvgaap vptqlkerpe mtarqrwhwa ynqivmqlrn gsgpggesgg rgnghlgdnp
     1441 fysnidsmpd irprrksvpl vseltmaatk rntaglasav pratlndeel kmhvykktlq
     1501 amiypisstt phkfvtwtat sptycyeceg llwgiarqgv rctecgvkch ekckdllnad
     1561 clqraaekss khgaedkans iitamkermk qrerekpeif elirttftvd pdthidsleq
     1621 aeqivlegts kwsckiaitv icaqgliakd ksgtsdpyvt vqvgkvkkrt rtmprelnpv
     1681 whekfyfech nssdrikvrv wdedndlksk lrqkltresd dflgqtiiev rtlsgemdvw
     1741 ynlekrtdks avsgairlhi sveikgeekv apyhvqytcl henlfhslce kndgvvalpq
     1801 akgddawkvy fdppgeelvd efamrygies iyqamthfhc lstkylcpgv pavmstllan
     1861 inayyahtsa ssavsasdrf aasnfgkekf vklldqlhns lridlsmyrn nfpassqekl
     1921 mdlkstvdll tsitffrmkv qelssppras tvvkdcvkac lrstyqflfe ncydiynref
     1981 qvdpseakrd aedhgpsles ldfwhklial ivsvieedkn syapvlnqfp qelnigqlsa
     2041 atmwslfavd mkyaleeheq hrlckssaym nlhfkvkwlh tnyvkdvppy kgavpeypaw
     2101 fepfvmqwln enddvsleyl hgafnrdkkd gfqkssehal fsvsvvdvft qltqcfdvvs
     2161 klecpdpeiw krymkrfakt ivkvlvayad ivkkefpshl keeriacilm nniqqlrvql
     2221 ekmfesmgge kleedaanil kelqqqlnga lddlamlfak slepritasv kelgdlllai
     2281 kgggqvqlsq paqrnnvave adevlrplmv lldgslslya escektvlkr llkelwkivm
     2341 rilektvvlp pmsdksmmfk nltdnaknla anakiedmsk lfknhmsgkp dvknalsgvm
     2401 diskeveknl spkqcavlev aldtikqyfh aggnglkkaf lekspelqsl ryalslytqt
     2461 tdtliktfvt sqvnqvplnk asqlrqrqrs msserggdeg egaegmesle eplrqskpsl
     2521 rresrqvpdt agtdegsvge vsiqvdlfth pgtgeqkvtv kvvaandlkw qlatgmfrpy
     2581 vevnligphl tgkkrkqstk sksnnwspqf nesfdfmign eqqqldfyel hicvkdycfa
     2641 redrlvgvav mqlkdiveeg scacwlslgk riqmdetgwt ilrilsqrnn deiakefvkl
     2701 ksdirqetpl pnpt
//
LOCUS       XP_076546734            2712 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_076546734
VERSION     XP_076546734.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690619.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2712
                     /product="unc-13 isoform X8"
                     /calculated_mol_wt=310084
     Region          1..187
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          257..>838
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <855..>1095
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1047..>1395
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1491..1572
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1632..1758
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1648,1654,1700,1702,1719)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1949..2466
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2555..2676
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2712
                     /gene="unc-13"
                     /coded_by="XM_076690619.1:118..8256"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne vtskqsynts kstvstgkqe
      121 qeqqqqqhis ldsmrdtnsn tqappdftnt egsgqwvsld selemrgaei igtkkptghs
      181 llidcrlelp fgkceqrtrw kslltsetam qlraarpmcg sdpenteaad lqrklemlnn
      241 mmdqearaeq arrqilynig hsgysedsdy tsdlnypvgg qganssasqf rtaanqlatp
      301 qrsletsren syerddhqip atvggrlaps nygnyggsgh sprydepype egppqrysqs
      361 qhasessepl fynsrprhyk eysqdgwyse gydyhgtrld etriysdtey ypstttsssr
      421 rraphrrpsl erqmtlyddh syytdgyssd grvgkmsaty pecqtdiryn eyydsitgyg
      481 yqdesrygqd dedrqwdsgg kwylgsstyy enkrnrktlp qrpassigih rpdyrptvtr
      541 qrsyeseeln ysshstrrrk plqpqqrpss rtegtgkklp ptpttpsnvv inrklaslpa
      601 tpsrqlpktr tpseesyyks dynedynyay rsqdnlpqdt yidsiyseqs gydqmhvpgk
      661 tqyaedsqyg ssytpqvddh ygrsyqepqt qdtydqtylq nsykdeyqkp yveqntqvyq
      721 dtyqdmtypn asqpndqyts qpndqyrkts rdmiiddnyq vmgfdknnvq yqdrtkvhpy
      781 kedtyqeqye dysvsvpssv ydqnnvtnty nqyqqdqyds qynintstgy qksypdtynq
      841 etyktevydq dtynqgtynq etykqdtynq etykpdtyke dtykqdtynq gtyiqdtynq
      901 etynqgtynq etypqdtytq dtynqvpits qsnyedaysk pqkdyvdyqt pytkeetvtt
      961 tykneyeeqy qdypdqyees yhdsypgsfe erykdspsas terrssevpe lsvttprgqt
     1021 rpngyqsses dyyftsqesq dlsstglsrr kklekrdasp lvqqntdsle srddelkesi
     1081 etavssissi pqkkvisdys tagdsspaap tltespqstv qpsttmvnhv tpnhvppsmt
     1141 vtamvhpttt angkrltrae syqeeviede eypeiipkep qrkdsqlshq sqlsqhssqh
     1201 sqhsqkpklt rgdsyasdhf peesysrrss yaqptrkdsf ssttqesfkp pltrtdsyqk
     1261 rgsygqnfta pqpisraesy qrgyfkdqds ieepevgisn avnddykmrd epleryerad
     1321 eamirgsreg slvdayktsp pmshtesprg sitkqaslee svqmdtpprs ppepppdeel
     1381 lemvgaapvp tqlkerpemt arqrwhwayn qivmqlrngs gpggesggrg nghlgdnpfy
     1441 snidsmpdir prrksvplvs eltmaatkrn taglasavpr atlndeelkm hvykktlqam
     1501 iypissttph kfvtwtatsp tycyecegll wgiarqgvrc tecgvkchek ckdllnadcl
     1561 qraaeksskh gaedkansii tamkermkqr erekpeifel irttftvdpd thidsleqae
     1621 qivlegtskw sckiaitvic aqgliakdks gtsdpyvtvq vgkvkkrtrt mprelnpvwh
     1681 ekfyfechns sdrikvrvwd edndlksklr qkltresddf lgqtiievrt lsgemdvwyn
     1741 lekrtdksav sgairlhisv eikgeekvap yhvqytclhe nlfhslcekn dgvvalpqak
     1801 gddawkvyfd ppgeelvdef amrygiesiy qamthfhcls tkylcpgvpa vmstllanin
     1861 ayyahtsass avsasdrfaa snfgkekfvk lldqlhnslr idlsmyrnnf passqeklmd
     1921 lkstvdllts itffrmkvqe lsspprastv vkdcvkaclr styqflfenc ydiynrefqv
     1981 dpseakrdae dhgpslesld fwhklialiv svieedknsy apvlnqfpqe lnigqlsaat
     2041 mwslfavdmk yaleeheqhr lckssaymnl hfkvkwlhtn yvkdvppykg avpeypawfe
     2101 pfvmqwlnen ddvsleylhg afnrdkkdgf qkssehalfs vsvvdvftql tqcfdvvskl
     2161 ecpdpeiwkr ymkrfaktiv kvlvayadiv kkefpshlke eriacilmnn iqqlrvqlek
     2221 mfesmggekl eedaanilke lqqqlngald dlamlfaksl epritasvke lgdlllaikg
     2281 ggqvqlsqpa qrnnvavead evlrplmvll dgslslyaes cektvlkrll kelwkivmri
     2341 lektvvlppm sdksmmfknl tdnaknlaan akiedmsklf knhmsgkpdv knalsgvmdi
     2401 skeveknlsp kqcavleval dtikqyfhag gnglkkafle kspelqslry alslytqttd
     2461 tliktfvtsq vnqvplnkas qlrqrqrsms serggdegeg aegmesleep lrqskpslrr
     2521 esrqvpdtag tdegsvgevs iqvdlfthpg tgeqkvtvkv vaandlkwql atgmfrpyve
     2581 vnligphltg kkrkqstksk snnwspqfne sfdfmigneq qqldfyelhi cvkdycfare
     2641 drlvgvavmq lkdiveegsc acwlslgkri qmdetgwtil rilsqrnnde iakefvklks
     2701 dirqetplpn pt
//
LOCUS       XP_076546735            2693 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_076546735
VERSION     XP_076546735.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690620.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2693
                     /product="unc-13 isoform X9"
                     /calculated_mol_wt=308249
     Region          1..187
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          234..>819
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <836..>1076
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1028..>1376
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1472..1553
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1613..1739
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1629,1635,1681,1683,1700)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1930..2447
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2536..2657
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2693
                     /gene="unc-13"
                     /coded_by="XM_076690620.1:118..8199"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne vtskqsynts kstvstgkqe
      121 qeqqqqqhis ldsmrdtnsn tqappdftnt egsgqwvsld selemrgaei igtkkptghs
      181 llidcrlelp fdpenteaad lqrklemlnn mmdqearaeq arrqilynig hsgysedsdy
      241 tsdlnypvgg qganssasqf rtaanqlatp qrsletsren syerddhqip atvggrlaps
      301 nygnyggsgh sprydepype egppqrysqs qhasessepl fynsrprhyk eyrrrkhtwd
      361 yedsqdgwys egydyhgtrl detriysdte yypstttsss rrraphrrps lerqmtlydd
      421 hsyytdgyss dgrvgkmsat ypecqtdiry neyydsitgy gyqdesrygq ddedrqwdsg
      481 gkwylgssty yenkrnrktl pqrpassigi hrpdyrptvt rqrsyeseel nysshstrrr
      541 kplqpqqrps srtegtgkkl pptpttpsnv vinrklaslp atpsrqlpkt rtpseesyyk
      601 sdynedynya yrsqdnlpqd tyidsiyseq sgydqmhvpg ktqyaedsqy gssytpqvdd
      661 hygrsyqepq tqdtydqtyl qnsykdeyqk pyveqntqvy qdtyqdmtyp nasqpndqyt
      721 sqpndqyrkt srdmiiddny qvmgfdknnv qyqdrtkvhp ykedtyqeqy edysvsvpss
      781 vydqnnvtnt ynqyqqdqyd sqynintstg yqksypdtyn qetyktevyd qdtynqgtyn
      841 qetykqdtyn qetykpdtyk edtykqdtyn qgtyiqdtyn qetynqgtyn qetypqdtyt
      901 qdtynqvpit sqsnyedays kpqkdyvdyq tpytkeetvt ttykneyeeq yqdypdqyee
      961 syhdsypgsf eerykdspsa sterrssevp elsvttprgq trpngyqsse sdyyftsqes
     1021 qdlsstglsr rkklekrdas plvqqntdsl esrddelkes ietavssiss ipqkkvisdy
     1081 stagdsspaa ptltespqst vqpsttmvnh vtpnhvppsm tvtamvhptt tangkrltra
     1141 esyqeevied eeypeiipke pqrkdsqlsh qsqlsqhssq hsqhsqkpkl trgdsyasdh
     1201 fpeesysrrs syaqptrkds fssttqesfk ppltrtdsyq krgsygqnft apqpisraes
     1261 yqrgyfkdqd sieepevgis navnddykmr depleryera deamirgsre gslvdaykts
     1321 ppmshtespr gsitkqasle esvqmdtppr sppepppdee llemvgaapv ptqlkerpem
     1381 tarqrwhway nqivmqlrng sgpggesggr gnghlgdnpf ysnidsmpdi rprrksvplv
     1441 seltmaatkr ntaglasavp ratlndeelk mhvykktlqa miypissttp hkfvtwtats
     1501 ptycyecegl lwgiarqgvr ctecgvkche kckdllnadc lqraaekssk hgaedkansi
     1561 itamkermkq rerekpeife lirttftvdp dthidsleqa eqivlegtsk wsckiaitvi
     1621 caqgliakdk sgtsdpyvtv qvgkvkkrtr tmprelnpvw hekfyfechn ssdrikvrvw
     1681 dedndlkskl rqkltresdd flgqtiievr tlsgemdvwy nlekrtdksa vsgairlhis
     1741 veikgeekva pyhvqytclh enlfhslcek ndgvvalpqa kgddawkvyf dppgeelvde
     1801 famrygiesi yqamthfhcl stkylcpgvp avmstllani nayyahtsas savsasdrfa
     1861 asnfgkekfv klldqlhnsl ridlsmyrnn fpassqeklm dlkstvdllt sitffrmkvq
     1921 elsspprast vvkdcvkacl rstyqflfen cydiynrefq vdpseakrda edhgpslesl
     1981 dfwhkliali vsvieedkns yapvlnqfpq elnigqlsaa tmwslfavdm kyaleeheqh
     2041 rlckssaymn lhfkvkwlht nyvkdvppyk gavpeypawf epfvmqwlne nddvsleylh
     2101 gafnrdkkdg fqkssehalf svsvvdvftq ltqcfdvvsk lecpdpeiwk rymkrfakti
     2161 vkvlvayadi vkkefpshlk eeriacilmn niqqlrvqle kmfesmggek leedaanilk
     2221 elqqqlngal ddlamlfaks lepritasvk elgdlllaik gggqvqlsqp aqrnnvavea
     2281 devlrplmvl ldgslslyae scektvlkrl lkelwkivmr ilektvvlpp msdksmmfkn
     2341 ltdnaknlaa nakiedmskl fknhmsgkpd vknalsgvmd iskeveknls pkqcavleva
     2401 ldtikqyfha ggnglkkafl ekspelqslr yalslytqtt dtliktfvts qvnqvplnka
     2461 sqlrqrqrsm sserggdege gaegmeslee plrqskpslr resrqvpdta gtdegsvgev
     2521 siqvdlfthp gtgeqkvtvk vvaandlkwq latgmfrpyv evnligphlt gkkrkqstks
     2581 ksnnwspqfn esfdfmigne qqqldfyelh icvkdycfar edrlvgvavm qlkdiveegs
     2641 cacwlslgkr iqmdetgwti lrilsqrnnd eiakefvklk sdirqetplp npt
//
LOCUS       XP_076546736            2673 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_076546736
VERSION     XP_076546736.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690621.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2673
                     /product="unc-13 isoform X10"
                     /calculated_mol_wt=306043
     Region          1..137
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          214..>799
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <816..>1056
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1008..>1356
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1452..1533
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1593..1719
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1609,1615,1661,1663,1680)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1910..2427
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2516..2637
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2673
                     /gene="unc-13"
                     /coded_by="XM_076690621.1:118..8139"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne egsgqwvsld selemrgaei
      121 igtkkptghs llidcrlelp fgkceqrtrw kslltsetam qlraarpmcg sdpenteaad
      181 lqrklemlnn mmdqearaeq arrqilynig hsgysedsdy tsdlnypvgg qganssasqf
      241 rtaanqlatp qrsletsren syerddhqip atvggrlaps nygnyggsgh sprydepype
      301 egppqrysqs qhasessepl fynsrprhyk eyrrrkhtwd yedsqdgwys egydyhgtrl
      361 detriysdte yypstttsss rrraphrrps lerqmtlydd hsyytdgyss dgrvgkmsat
      421 ypecqtdiry neyydsitgy gyqdesrygq ddedrqwdsg gkwylgssty yenkrnrktl
      481 pqrpassigi hrpdyrptvt rqrsyeseel nysshstrrr kplqpqqrps srtegtgkkl
      541 pptpttpsnv vinrklaslp atpsrqlpkt rtpseesyyk sdynedynya yrsqdnlpqd
      601 tyidsiyseq sgydqmhvpg ktqyaedsqy gssytpqvdd hygrsyqepq tqdtydqtyl
      661 qnsykdeyqk pyveqntqvy qdtyqdmtyp nasqpndqyt sqpndqyrkt srdmiiddny
      721 qvmgfdknnv qyqdrtkvhp ykedtyqeqy edysvsvpss vydqnnvtnt ynqyqqdqyd
      781 sqynintstg yqksypdtyn qetyktevyd qdtynqgtyn qetykqdtyn qetykpdtyk
      841 edtykqdtyn qgtyiqdtyn qetynqgtyn qetypqdtyt qdtynqvpit sqsnyedays
      901 kpqkdyvdyq tpytkeetvt ttykneyeeq yqdypdqyee syhdsypgsf eerykdspsa
      961 sterrssevp elsvttprgq trpngyqsse sdyyftsqes qdlsstglsr rkklekrdas
     1021 plvqqntdsl esrddelkes ietavssiss ipqkkvisdy stagdsspaa ptltespqst
     1081 vqpsttmvnh vtpnhvppsm tvtamvhptt tangkrltra esyqeevied eeypeiipke
     1141 pqrkdsqlsh qsqlsqhssq hsqhsqkpkl trgdsyasdh fpeesysrrs syaqptrkds
     1201 fssttqesfk ppltrtdsyq krgsygqnft apqpisraes yqrgyfkdqd sieepevgis
     1261 navnddykmr depleryera deamirgsre gslvdaykts ppmshtespr gsitkqasle
     1321 esvqmdtppr sppepppdee llemvgaapv ptqlkerpem tarqrwhway nqivmqlrng
     1381 sgpggesggr gnghlgdnpf ysnidsmpdi rprrksvplv seltmaatkr ntaglasavp
     1441 ratlndeelk mhvykktlqa miypissttp hkfvtwtats ptycyecegl lwgiarqgvr
     1501 ctecgvkche kckdllnadc lqraaekssk hgaedkansi itamkermkq rerekpeife
     1561 lirttftvdp dthidsleqa eqivlegtsk wsckiaitvi caqgliakdk sgtsdpyvtv
     1621 qvgkvkkrtr tmprelnpvw hekfyfechn ssdrikvrvw dedndlkskl rqkltresdd
     1681 flgqtiievr tlsgemdvwy nlekrtdksa vsgairlhis veikgeekva pyhvqytclh
     1741 enlfhslcek ndgvvalpqa kgddawkvyf dppgeelvde famrygiesi yqamthfhcl
     1801 stkylcpgvp avmstllani nayyahtsas savsasdrfa asnfgkekfv klldqlhnsl
     1861 ridlsmyrnn fpassqeklm dlkstvdllt sitffrmkvq elsspprast vvkdcvkacl
     1921 rstyqflfen cydiynrefq vdpseakrda edhgpslesl dfwhkliali vsvieedkns
     1981 yapvlnqfpq elnigqlsaa tmwslfavdm kyaleeheqh rlckssaymn lhfkvkwlht
     2041 nyvkdvppyk gavpeypawf epfvmqwlne nddvsleylh gafnrdkkdg fqkssehalf
     2101 svsvvdvftq ltqcfdvvsk lecpdpeiwk rymkrfakti vkvlvayadi vkkefpshlk
     2161 eeriacilmn niqqlrvqle kmfesmggek leedaanilk elqqqlngal ddlamlfaks
     2221 lepritasvk elgdlllaik gggqvqlsqp aqrnnvavea devlrplmvl ldgslslyae
     2281 scektvlkrl lkelwkivmr ilektvvlpp msdksmmfkn ltdnaknlaa nakiedmskl
     2341 fknhmsgkpd vknalsgvmd iskeveknls pkqcavleva ldtikqyfha ggnglkkafl
     2401 ekspelqslr yalslytqtt dtliktfvts qvnqvplnka sqlrqrqrsm sserggdege
     2461 gaegmeslee plrqskpslr resrqvpdta gtdegsvgev siqvdlfthp gtgeqkvtvk
     2521 vvaandlkwq latgmfrpyv evnligphlt gkkrkqstks ksnnwspqfn esfdfmigne
     2581 qqqldfyelh icvkdycfar edrlvgvavm qlkdiveegs cacwlslgkr iqmdetgwti
     2641 lrilsqrnnd eiakefvklk sdirqetplp npt
//
LOCUS       XP_034195913            2672 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_034195913
VERSION     XP_034195913.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340022.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2672
                     /product="unc-13 isoform X11"
                     /calculated_mol_wt=305877
     Region          1..187
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          264..>849
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <866..>1106
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1058..>1406
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1502..1583
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1643..1769
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1659,1665,1711,1713,1730)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1960..2477
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2515..2636
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2672
                     /gene="unc-13"
                     /coded_by="XM_034340022.2:118..8136"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne vtskqsynts kstvstgkqe
      121 qeqqqqqhis ldsmrdtnsn tqappdftnt egsgqwvsld selemrgaei igtkkptghs
      181 llidcrlelp fgkceqrtrw kslltsetam qlraarpmcg sdpenteaad lqrklemlnn
      241 mmdqearaeq arrqilynig hsgysedsdy tsdlnypvgg qganssasqf rtaanqlatp
      301 qrsletsren syerddhqip atvggrlaps nygnyggsgh sprydepype egppqrysqs
      361 qhasessepl fynsrprhyk eyrrrkhtwd yedsqdgwys egydyhgtrl detriysdte
      421 yypstttsss rrraphrrps lerqmtlydd hsyytdgyss dgrvgkmsat ypecqtdiry
      481 neyydsitgy gyqdesrygq ddedrqwdsg gkwylgssty yenkrnrktl pqrpassigi
      541 hrpdyrptvt rqrsyeseel nysshstrrr kplqpqqrps srtegtgkkl pptpttpsnv
      601 vinrklaslp atpsrqlpkt rtpseesyyk sdynedynya yrsqdnlpqd tyidsiyseq
      661 sgydqmhvpg ktqyaedsqy gssytpqvdd hygrsyqepq tqdtydqtyl qnsykdeyqk
      721 pyveqntqvy qdtyqdmtyp nasqpndqyt sqpndqyrkt srdmiiddny qvmgfdknnv
      781 qyqdrtkvhp ykedtyqeqy edysvsvpss vydqnnvtnt ynqyqqdqyd sqynintstg
      841 yqksypdtyn qetyktevyd qdtynqgtyn qetykqdtyn qetykpdtyk edtykqdtyn
      901 qgtyiqdtyn qetynqgtyn qetypqdtyt qdtynqvpit sqsnyedays kpqkdyvdyq
      961 tpytkeetvt ttykneyeeq yqdypdqyee syhdsypgsf eerykdspsa sterrssevp
     1021 elsvttprgq trpngyqsse sdyyftsqes qdlsstglsr rkklekrdas plvqqntdsl
     1081 esrddelkes ietavssiss ipqkkvisdy stagdsspaa ptltespqst vqpsttmvnh
     1141 vtpnhvppsm tvtamvhptt tangkrltra esyqeevied eeypeiipke pqrkdsqlsh
     1201 qsqlsqhssq hsqhsqkpkl trgdsyasdh fpeesysrrs syaqptrkds fssttqesfk
     1261 ppltrtdsyq krgsygqnft apqpisraes yqrgyfkdqd sieepevgis navnddykmr
     1321 depleryera deamirgsre gslvdaykts ppmshtespr gsitkqasle esvqmdtppr
     1381 sppepppdee llemvgaapv ptqlkerpem tarqrwhway nqivmqlrng sgpggesggr
     1441 gnghlgdnpf ysnidsmpdi rprrksvplv seltmaatkr ntaglasavp ratlndeelk
     1501 mhvykktlqa miypissttp hkfvtwtats ptycyecegl lwgiarqgvr ctecgvkche
     1561 kckdllnadc lqraaekssk hgaedkansi itamkermkq rerekpeife lirttftvdp
     1621 dthidsleqa eqivlegtsk wsckiaitvi caqgliakdk sgtsdpyvtv qvgkvkkrtr
     1681 tmprelnpvw hekfyfechn ssdrikvrvw dedndlkskl rqkltresdd flgqtiievr
     1741 tlsgemdvwy nlekrtdksa vsgairlhis veikgeekva pyhvqytclh enlfhslcek
     1801 ndgvvalpqa kgddawkvyf dppgeelvde famrygiesi yqamthfhcl stkylcpgvp
     1861 avmstllani nayyahtsas savsasdrfa asnfgkekfv klldqlhnsl ridlsmyrnn
     1921 fpassqeklm dlkstvdllt sitffrmkvq elsspprast vvkdcvkacl rstyqflfen
     1981 cydiynrefq vdpseakrda edhgpslesl dfwhkliali vsvieedkns yapvlnqfpq
     2041 elnigqlsaa tmwslfavdm kyaleeheqh rlckssaymn lhfkvkwlht nyvkdvppyk
     2101 gavpeypawf epfvmqwlne nddvsleylh gafnrdkkdg fqkssehalf svsvvdvftq
     2161 ltqcfdvvsk lecpdpeiwk rymkrfakti vkvlvayadi vkkefpshlk eeriacilmn
     2221 niqqlrvqle kmfesmggek leedaanilk elqqqlngal ddlamlfaks lepritasvk
     2281 elgdlllaik gggqvqlsqp aqrnnvavea devlrplmvl ldgslslyae scektvlkrl
     2341 lkelwkivmr ilektvvlpp msdksmmfkn ltdnaknlaa nakiedmskl fknhmsgkpd
     2401 vknalsgvmd iskeveknls pkqcavleva ldtikqyfha ggnglkkafl ekspelqslr
     2461 yalslytqtt dtliktfvts qvnqvpdtag tdegsvgevs iqvdlfthpg tgeqkvtvkv
     2521 vaandlkwql atgmfrpyve vnligphltg kkrkqstksk snnwspqfne sfdfmigneq
     2581 qqldfyelhi cvkdycfare drlvgvavmq lkdiveegsc acwlslgkri qmdetgwtil
     2641 rilsqrnnde iakefvklks dirqetplpn pt
//
LOCUS       XP_076546737            2653 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X12 [Osmia lignaria lignaria].
ACCESSION   XP_076546737
VERSION     XP_076546737.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690622.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2653
                     /product="unc-13 isoform X12"
                     /calculated_mol_wt=303537
     Region          1..187
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          21..>779
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <796..>1036
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          988..>1336
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1432..1513
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1573..1699
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1589,1595,1641,1643,1660)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1890..2407
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2496..2617
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2653
                     /gene="unc-13"
                     /coded_by="XM_076690622.1:118..8079"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne vtskqsynts kstvstgkqe
      121 qeqqqqqhis ldsmrdtnsn tqappdftnt egsgqwvsld selemrgaei igtkkptghs
      181 llidcrlelp fagysedsdy tsdlnypvgg qganssasqf rtaanqlatp qrsletsren
      241 syerddhqip atvggrlaps nygnyggsgh sprydepype egppqrysqs qhasessepl
      301 fynsrprhyk eyrrrkhtwd yedsqdgwys egydyhgtrl detriysdte yypstttsss
      361 rrraphrrps lerqmtlydd hsyytdgyss dgrvgkmsat ypecqtdiry neyydsitgy
      421 gyqdesrygq ddedrqwdsg gkwylgssty yenkrnrktl pqrpassigi hrpdyrptvt
      481 rqrsyeseel nysshstrrr kplqpqqrps srtegtgkkl pptpttpsnv vinrklaslp
      541 atpsrqlpkt rtpseesyyk sdynedynya yrsqdnlpqd tyidsiyseq sgydqmhvpg
      601 ktqyaedsqy gssytpqvdd hygrsyqepq tqdtydqtyl qnsykdeyqk pyveqntqvy
      661 qdtyqdmtyp nasqpndqyt sqpndqyrkt srdmiiddny qvmgfdknnv qyqdrtkvhp
      721 ykedtyqeqy edysvsvpss vydqnnvtnt ynqyqqdqyd sqynintstg yqksypdtyn
      781 qetyktevyd qdtynqgtyn qetykqdtyn qetykpdtyk edtykqdtyn qgtyiqdtyn
      841 qetynqgtyn qetypqdtyt qdtynqvpit sqsnyedays kpqkdyvdyq tpytkeetvt
      901 ttykneyeeq yqdypdqyee syhdsypgsf eerykdspsa sterrssevp elsvttprgq
      961 trpngyqsse sdyyftsqes qdlsstglsr rkklekrdas plvqqntdsl esrddelkes
     1021 ietavssiss ipqkkvisdy stagdsspaa ptltespqst vqpsttmvnh vtpnhvppsm
     1081 tvtamvhptt tangkrltra esyqeevied eeypeiipke pqrkdsqlsh qsqlsqhssq
     1141 hsqhsqkpkl trgdsyasdh fpeesysrrs syaqptrkds fssttqesfk ppltrtdsyq
     1201 krgsygqnft apqpisraes yqrgyfkdqd sieepevgis navnddykmr depleryera
     1261 deamirgsre gslvdaykts ppmshtespr gsitkqasle esvqmdtppr sppepppdee
     1321 llemvgaapv ptqlkerpem tarqrwhway nqivmqlrng sgpggesggr gnghlgdnpf
     1381 ysnidsmpdi rprrksvplv seltmaatkr ntaglasavp ratlndeelk mhvykktlqa
     1441 miypissttp hkfvtwtats ptycyecegl lwgiarqgvr ctecgvkche kckdllnadc
     1501 lqraaekssk hgaedkansi itamkermkq rerekpeife lirttftvdp dthidsleqa
     1561 eqivlegtsk wsckiaitvi caqgliakdk sgtsdpyvtv qvgkvkkrtr tmprelnpvw
     1621 hekfyfechn ssdrikvrvw dedndlkskl rqkltresdd flgqtiievr tlsgemdvwy
     1681 nlekrtdksa vsgairlhis veikgeekva pyhvqytclh enlfhslcek ndgvvalpqa
     1741 kgddawkvyf dppgeelvde famrygiesi yqamthfhcl stkylcpgvp avmstllani
     1801 nayyahtsas savsasdrfa asnfgkekfv klldqlhnsl ridlsmyrnn fpassqeklm
     1861 dlkstvdllt sitffrmkvq elsspprast vvkdcvkacl rstyqflfen cydiynrefq
     1921 vdpseakrda edhgpslesl dfwhkliali vsvieedkns yapvlnqfpq elnigqlsaa
     1981 tmwslfavdm kyaleeheqh rlckssaymn lhfkvkwlht nyvkdvppyk gavpeypawf
     2041 epfvmqwlne nddvsleylh gafnrdkkdg fqkssehalf svsvvdvftq ltqcfdvvsk
     2101 lecpdpeiwk rymkrfakti vkvlvayadi vkkefpshlk eeriacilmn niqqlrvqle
     2161 kmfesmggek leedaanilk elqqqlngal ddlamlfaks lepritasvk elgdlllaik
     2221 gggqvqlsqp aqrnnvavea devlrplmvl ldgslslyae scektvlkrl lkelwkivmr
     2281 ilektvvlpp msdksmmfkn ltdnaknlaa nakiedmskl fknhmsgkpd vknalsgvmd
     2341 iskeveknls pkqcavleva ldtikqyfha ggnglkkafl ekspelqslr yalslytqtt
     2401 dtliktfvts qvnqvplnka sqlrqrqrsm sserggdege gaegmeslee plrqskpslr
     2461 resrqvpdta gtdegsvgev siqvdlfthp gtgeqkvtvk vvaandlkwq latgmfrpyv
     2521 evnligphlt gkkrkqstks ksnnwspqfn esfdfmigne qqqldfyelh icvkdycfar
     2581 edrlvgvavm qlkdiveegs cacwlslgkr iqmdetgwti lrilsqrnnd eiakefvklk
     2641 sdirqetplp npt
//
LOCUS       XP_076546738            2643 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X13 [Osmia lignaria lignaria].
ACCESSION   XP_076546738
VERSION     XP_076546738.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690623.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2643
                     /product="unc-13 isoform X13"
                     /calculated_mol_wt=302664
     Region          1..137
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          184..>769
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <786..>1026
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          978..>1326
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1422..1503
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1563..1689
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1579,1585,1631,1633,1650)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1880..2397
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2486..2607
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2643
                     /gene="unc-13"
                     /coded_by="XM_076690623.1:118..8049"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne egsgqwvsld selemrgaei
      121 igtkkptghs llidcrlelp fdpenteaad lqrklemlnn mmdqearaeq arrqilynig
      181 hsgysedsdy tsdlnypvgg qganssasqf rtaanqlatp qrsletsren syerddhqip
      241 atvggrlaps nygnyggsgh sprydepype egppqrysqs qhasessepl fynsrprhyk
      301 eyrrrkhtwd yedsqdgwys egydyhgtrl detriysdte yypstttsss rrraphrrps
      361 lerqmtlydd hsyytdgyss dgrvgkmsat ypecqtdiry neyydsitgy gyqdesrygq
      421 ddedrqwdsg gkwylgssty yenkrnrktl pqrpassigi hrpdyrptvt rqrsyeseel
      481 nysshstrrr kplqpqqrps srtegtgkkl pptpttpsnv vinrklaslp atpsrqlpkt
      541 rtpseesyyk sdynedynya yrsqdnlpqd tyidsiyseq sgydqmhvpg ktqyaedsqy
      601 gssytpqvdd hygrsyqepq tqdtydqtyl qnsykdeyqk pyveqntqvy qdtyqdmtyp
      661 nasqpndqyt sqpndqyrkt srdmiiddny qvmgfdknnv qyqdrtkvhp ykedtyqeqy
      721 edysvsvpss vydqnnvtnt ynqyqqdqyd sqynintstg yqksypdtyn qetyktevyd
      781 qdtynqgtyn qetykqdtyn qetykpdtyk edtykqdtyn qgtyiqdtyn qetynqgtyn
      841 qetypqdtyt qdtynqvpit sqsnyedays kpqkdyvdyq tpytkeetvt ttykneyeeq
      901 yqdypdqyee syhdsypgsf eerykdspsa sterrssevp elsvttprgq trpngyqsse
      961 sdyyftsqes qdlsstglsr rkklekrdas plvqqntdsl esrddelkes ietavssiss
     1021 ipqkkvisdy stagdsspaa ptltespqst vqpsttmvnh vtpnhvppsm tvtamvhptt
     1081 tangkrltra esyqeevied eeypeiipke pqrkdsqlsh qsqlsqhssq hsqhsqkpkl
     1141 trgdsyasdh fpeesysrrs syaqptrkds fssttqesfk ppltrtdsyq krgsygqnft
     1201 apqpisraes yqrgyfkdqd sieepevgis navnddykmr depleryera deamirgsre
     1261 gslvdaykts ppmshtespr gsitkqasle esvqmdtppr sppepppdee llemvgaapv
     1321 ptqlkerpem tarqrwhway nqivmqlrng sgpggesggr gnghlgdnpf ysnidsmpdi
     1381 rprrksvplv seltmaatkr ntaglasavp ratlndeelk mhvykktlqa miypissttp
     1441 hkfvtwtats ptycyecegl lwgiarqgvr ctecgvkche kckdllnadc lqraaekssk
     1501 hgaedkansi itamkermkq rerekpeife lirttftvdp dthidsleqa eqivlegtsk
     1561 wsckiaitvi caqgliakdk sgtsdpyvtv qvgkvkkrtr tmprelnpvw hekfyfechn
     1621 ssdrikvrvw dedndlkskl rqkltresdd flgqtiievr tlsgemdvwy nlekrtdksa
     1681 vsgairlhis veikgeekva pyhvqytclh enlfhslcek ndgvvalpqa kgddawkvyf
     1741 dppgeelvde famrygiesi yqamthfhcl stkylcpgvp avmstllani nayyahtsas
     1801 savsasdrfa asnfgkekfv klldqlhnsl ridlsmyrnn fpassqeklm dlkstvdllt
     1861 sitffrmkvq elsspprast vvkdcvkacl rstyqflfen cydiynrefq vdpseakrda
     1921 edhgpslesl dfwhkliali vsvieedkns yapvlnqfpq elnigqlsaa tmwslfavdm
     1981 kyaleeheqh rlckssaymn lhfkvkwlht nyvkdvppyk gavpeypawf epfvmqwlne
     2041 nddvsleylh gafnrdkkdg fqkssehalf svsvvdvftq ltqcfdvvsk lecpdpeiwk
     2101 rymkrfakti vkvlvayadi vkkefpshlk eeriacilmn niqqlrvqle kmfesmggek
     2161 leedaanilk elqqqlngal ddlamlfaks lepritasvk elgdlllaik gggqvqlsqp
     2221 aqrnnvavea devlrplmvl ldgslslyae scektvlkrl lkelwkivmr ilektvvlpp
     2281 msdksmmfkn ltdnaknlaa nakiedmskl fknhmsgkpd vknalsgvmd iskeveknls
     2341 pkqcavleva ldtikqyfha ggnglkkafl ekspelqslr yalslytqtt dtliktfvts
     2401 qvnqvplnka sqlrqrqrsm sserggdege gaegmeslee plrqskpslr resrqvpdta
     2461 gtdegsvgev siqvdlfthp gtgeqkvtvk vvaandlkwq latgmfrpyv evnligphlt
     2521 gkkrkqstks ksnnwspqfn esfdfmigne qqqldfyelh icvkdycfar edrlvgvavm
     2581 qlkdiveegs cacwlslgkr iqmdetgwti lrilsqrnnd eiakefvklk sdirqetplp
     2641 npt
//
LOCUS       XP_076546739            2603 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X14 [Osmia lignaria lignaria].
ACCESSION   XP_076546739
VERSION     XP_076546739.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690624.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2603
                     /product="unc-13 isoform X14"
                     /calculated_mol_wt=297952
     Region          1..137
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          140..>729
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <746..>986
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          938..>1286
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1382..1463
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1523..1649
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1539,1545,1591,1593,1610)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1840..2357
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2446..2567
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2603
                     /gene="unc-13"
                     /coded_by="XM_076690624.1:118..7929"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne egsgqwvsld selemrgaei
      121 igtkkptghs llidcrlelp fagysedsdy tsdlnypvgg qganssasqf rtaanqlatp
      181 qrsletsren syerddhqip atvggrlaps nygnyggsgh sprydepype egppqrysqs
      241 qhasessepl fynsrprhyk eyrrrkhtwd yedsqdgwys egydyhgtrl detriysdte
      301 yypstttsss rrraphrrps lerqmtlydd hsyytdgyss dgrvgkmsat ypecqtdiry
      361 neyydsitgy gyqdesrygq ddedrqwdsg gkwylgssty yenkrnrktl pqrpassigi
      421 hrpdyrptvt rqrsyeseel nysshstrrr kplqpqqrps srtegtgkkl pptpttpsnv
      481 vinrklaslp atpsrqlpkt rtpseesyyk sdynedynya yrsqdnlpqd tyidsiyseq
      541 sgydqmhvpg ktqyaedsqy gssytpqvdd hygrsyqepq tqdtydqtyl qnsykdeyqk
      601 pyveqntqvy qdtyqdmtyp nasqpndqyt sqpndqyrkt srdmiiddny qvmgfdknnv
      661 qyqdrtkvhp ykedtyqeqy edysvsvpss vydqnnvtnt ynqyqqdqyd sqynintstg
      721 yqksypdtyn qetyktevyd qdtynqgtyn qetykqdtyn qetykpdtyk edtykqdtyn
      781 qgtyiqdtyn qetynqgtyn qetypqdtyt qdtynqvpit sqsnyedays kpqkdyvdyq
      841 tpytkeetvt ttykneyeeq yqdypdqyee syhdsypgsf eerykdspsa sterrssevp
      901 elsvttprgq trpngyqsse sdyyftsqes qdlsstglsr rkklekrdas plvqqntdsl
      961 esrddelkes ietavssiss ipqkkvisdy stagdsspaa ptltespqst vqpsttmvnh
     1021 vtpnhvppsm tvtamvhptt tangkrltra esyqeevied eeypeiipke pqrkdsqlsh
     1081 qsqlsqhssq hsqhsqkpkl trgdsyasdh fpeesysrrs syaqptrkds fssttqesfk
     1141 ppltrtdsyq krgsygqnft apqpisraes yqrgyfkdqd sieepevgis navnddykmr
     1201 depleryera deamirgsre gslvdaykts ppmshtespr gsitkqasle esvqmdtppr
     1261 sppepppdee llemvgaapv ptqlkerpem tarqrwhway nqivmqlrng sgpggesggr
     1321 gnghlgdnpf ysnidsmpdi rprrksvplv seltmaatkr ntaglasavp ratlndeelk
     1381 mhvykktlqa miypissttp hkfvtwtats ptycyecegl lwgiarqgvr ctecgvkche
     1441 kckdllnadc lqraaekssk hgaedkansi itamkermkq rerekpeife lirttftvdp
     1501 dthidsleqa eqivlegtsk wsckiaitvi caqgliakdk sgtsdpyvtv qvgkvkkrtr
     1561 tmprelnpvw hekfyfechn ssdrikvrvw dedndlkskl rqkltresdd flgqtiievr
     1621 tlsgemdvwy nlekrtdksa vsgairlhis veikgeekva pyhvqytclh enlfhslcek
     1681 ndgvvalpqa kgddawkvyf dppgeelvde famrygiesi yqamthfhcl stkylcpgvp
     1741 avmstllani nayyahtsas savsasdrfa asnfgkekfv klldqlhnsl ridlsmyrnn
     1801 fpassqeklm dlkstvdllt sitffrmkvq elsspprast vvkdcvkacl rstyqflfen
     1861 cydiynrefq vdpseakrda edhgpslesl dfwhkliali vsvieedkns yapvlnqfpq
     1921 elnigqlsaa tmwslfavdm kyaleeheqh rlckssaymn lhfkvkwlht nyvkdvppyk
     1981 gavpeypawf epfvmqwlne nddvsleylh gafnrdkkdg fqkssehalf svsvvdvftq
     2041 ltqcfdvvsk lecpdpeiwk rymkrfakti vkvlvayadi vkkefpshlk eeriacilmn
     2101 niqqlrvqle kmfesmggek leedaanilk elqqqlngal ddlamlfaks lepritasvk
     2161 elgdlllaik gggqvqlsqp aqrnnvavea devlrplmvl ldgslslyae scektvlkrl
     2221 lkelwkivmr ilektvvlpp msdksmmfkn ltdnaknlaa nakiedmskl fknhmsgkpd
     2281 vknalsgvmd iskeveknls pkqcavleva ldtikqyfha ggnglkkafl ekspelqslr
     2341 yalslytqtt dtliktfvts qvnqvplnka sqlrqrqrsm sserggdege gaegmeslee
     2401 plrqskpslr resrqvpdta gtdegsvgev siqvdlfthp gtgeqkvtvk vvaandlkwq
     2461 latgmfrpyv evnligphlt gkkrkqstks ksnnwspqfn esfdfmigne qqqldfyelh
     2521 icvkdycfar edrlvgvavm qlkdiveegs cacwlslgkr iqmdetgwti lrilsqrnnd
     2581 eiakefvklk sdirqetplp npt
//
LOCUS       XP_076546740            2713 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X15 [Osmia lignaria lignaria].
ACCESSION   XP_076546740
VERSION     XP_076546740.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690625.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2713
                     /product="unc-13 isoform X15"
                     /calculated_mol_wt=310578
     Region          1..178
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          251..>839
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <856..>1096
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1048..>1396
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1492..1573
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1633..1759
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1649,1655,1701,1703,1720)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1950..2467
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2556..2677
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2713
                     /gene="unc-13"
                     /coded_by="XM_076690625.1:118..8259"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceeadq fnsyvtlklq nvksttvtvk ganpcweqdf lfetndmntg
       61 llvevwckgf lwdrflgyyy vplsevsymn evtskqsynt skstvstgkq eqeqqqqqhi
      121 sldsmrdtns ntqappdftn tegsgqwvsl dselemrgae iigtkkptgh sllidcrlel
      181 pfgkceqrtr wkslltseta mqlraarpmc gsdpenteaa dlqrklemln nmmdqearae
      241 qarrqilyni ghsgysedsd ytsdlnypvg gqganssasq frtaanqlat pqrsletsre
      301 nsyerddhqi patvggrlap snygnyggsg hsprydepyp eegppqrysq sqhasessep
      361 lfynsrprhy keyrrrkhtw dyedsqdgwy segydyhgtr ldetriysdt eyypstttss
      421 srrraphrrp slerqmtlyd dhsyytdgys sdgrvgkmsa typecqtdir yneyydsitg
      481 ygyqderygq ddedrqwdsg gkwylgssty yenkrnrktl pqrpassigi hrpdyrptvt
      541 rqrsyeseel nysshstrrr kplqpqqrps srtegtgkkl pptpttpsnv vinrklaslp
      601 atpsrqlpkt rtpseesyyk sdynedynya yrsqdnlpqd tyidsiyseq sgydqmhvpg
      661 ktqyaedsqy gssytpqvdd hygrsyqepq tqdtydqtyl qnsykdeyqk pyveqntqvy
      721 qdtyqdmtyp nasqpndqyt sqpndqyrkt srdmiiddny qvmgfdknnv qyqdrtkvhp
      781 ykedtyqeqy edysvsvpss vydqnnvtnt ynqyqqdqyd sqynintstg yqksypdtyn
      841 qetyktevyd qdtynqgtyn qetykqdtyn qetykpdtyk edtykqdtyn qgtyiqdtyn
      901 qetynqgtyn qetypqdtyt qdtynqvpit sqsnyedays kpqkdyvdyq tpytkeetvt
      961 ttykneyeeq yqdypdqyee syhdsypgsf eerykdspsa sterrssevp elsvttprgq
     1021 trpngyqsse sdyyftsqes qdlsstglsr rkklekrdas plvqqntdsl esrddelkes
     1081 ietavssiss ipqkkvisdy stagdsspaa ptltespqst vqpsttmvnh vtpnhvppsm
     1141 tvtamvhptt tangkrltra esyqeevied eeypeiipke pqrkdsqlsh qsqlsqhssq
     1201 hsqhsqkpkl trgdsyasdh fpeesysrrs syaqptrkds fssttqesfk ppltrtdsyq
     1261 krgsygqnft apqpisraes yqrgyfkdqd sieepevgis navnddykmr depleryera
     1321 deamirgsre gslvdaykts ppmshtespr gsitkqasle esvqmdtppr sppepppdee
     1381 llemvgaapv ptqlkerpem tarqrwhway nqivmqlrng sgpggesggr gnghlgdnpf
     1441 ysnidsmpdi rprrksvplv seltmaatkr ntaglasavp ratlndeelk mhvykktlqa
     1501 miypissttp hkfvtwtats ptycyecegl lwgiarqgvr ctecgvkche kckdllnadc
     1561 lqraaekssk hgaedkansi itamkermkq rerekpeife lirttftvdp dthidsleqa
     1621 eqivlegtsk wsckiaitvi caqgliakdk sgtsdpyvtv qvgkvkkrtr tmprelnpvw
     1681 hekfyfechn ssdrikvrvw dedndlkskl rqkltresdd flgqtiievr tlsgemdvwy
     1741 nlekrtdksa vsgairlhis veikgeekva pyhvqytclh enlfhslcek ndgvvalpqa
     1801 kgddawkvyf dppgeelvde famrygiesi yqamthfhcl stkylcpgvp avmstllani
     1861 nayyahtsas savsasdrfa asnfgkekfv klldqlhnsl ridlsmyrnn fpassqeklm
     1921 dlkstvdllt sitffrmkvq elsspprast vvkdcvkacl rstyqflfen cydiynrefq
     1981 vdpseakrda edhgpslesl dfwhkliali vsvieedkns yapvlnqfpq elnigqlsaa
     2041 tmwslfavdm kyaleeheqh rlckssaymn lhfkvkwlht nyvkdvppyk gavpeypawf
     2101 epfvmqwlne nddvsleylh gafnrdkkdg fqkssehalf svsvvdvftq ltqcfdvvsk
     2161 lecpdpeiwk rymkrfakti vkvlvayadi vkkefpshlk eeriacilmn niqqlrvqle
     2221 kmfesmggek leedaanilk elqqqlngal ddlamlfaks lepritasvk elgdlllaik
     2281 gggqvqlsqp aqrnnvavea devlrplmvl ldgslslyae scektvlkrl lkelwkivmr
     2341 ilektvvlpp msdksmmfkn ltdnaknlaa nakiedmskl fknhmsgkpd vknalsgvmd
     2401 iskeveknls pkqcavleva ldtikqyfha ggnglkkafl ekspelqslr yalslytqtt
     2461 dtliktfvts qvnqvplnka sqlrqrqrsm sserggdege gaegmeslee plrqskpslr
     2521 resrqvpdta gtdegsvgev siqvdlfthp gtgeqkvtvk vvaandlkwq latgmfrpyv
     2581 evnligphlt gkkrkqstks ksnnwspqfn esfdfmigne qqqldfyelh icvkdycfar
     2641 edrlvgvavm qlkdiveegs cacwlslgkr iqmdetgwti lrilsqrnnd eiakefvklk
     2701 sdirqetplp npt
//
LOCUS       XP_076546741            2723 aa            linear   INV 03-SEP-2025
DEFINITION  unc-13 isoform X16 [Osmia lignaria lignaria].
ACCESSION   XP_076546741
VERSION     XP_076546741.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690626.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2723
                     /product="unc-13 isoform X16"
                     /calculated_mol_wt=311583
     Region          1..187
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          264..>849
                     /region_name="RhsA"
                     /note="Uncharacterized conserved protein RhaS, contains 28
                     RHS repeats [General function prediction only]; COG3209"
                     /db_xref="CDD:442442"
     Region          <866..>1106
                     /region_name="PTZ00395"
                     /note="Sec24-related protein; Provisional"
                     /db_xref="CDD:185594"
     Region          1058..>1406
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          1502..1583
                     /region_name="C1"
                     /note="protein kinase C conserved region 1 (C1 domain)
                     superfamily; cl00040"
                     /db_xref="CDD:412127"
     Region          1643..1769
                     /region_name="C2B_Munc13"
                     /note="C2 domain second repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd04027"
                     /db_xref="CDD:175993"
     Site            order(1659,1665,1711,1713,1730)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175993"
     Region          1960..2477
                     /region_name="MUN"
                     /note="MUN domain; pfam06292"
                     /db_xref="CDD:461870"
     Region          2566..2687
                     /region_name="C2C_Munc13"
                     /note="C2 domain third repeat in Munc13 (mammalian
                     uncoordinated) proteins; cd08395"
                     /db_xref="CDD:176041"
     CDS             1..2723
                     /gene="unc-13"
                     /coded_by="XM_076690626.1:118..8289"
                     /db_xref="GeneID:117611769"
ORIGIN      
        1 msllsvtvkk arfvceedse rsttvsadqf nsyvtlklqn vksttvtvkg anpcweqdfl
       61 fetndmntgl lvevwckgfl wdrflgyyyv plsevsymne vtskqsynts kstvstgkqe
      121 qeqqqqqhis ldsmrdtnsn tqappdftnt egsgqwvsld selemrgaei igtkkptghs
      181 llidcrlelp fgkceqrtrw kslltsetam qlraarpmcg sdpenteaad lqrklemlnn
      241 mmdqearaeq arrqilynig hsgysedsdy tsdlnypvgg qganssasqf rtaanqlatp
      301 qrsletsren syerddhqip atvggrlaps nygnyggsgh sprydepype egppqrysqs
      361 qhasessepl fynsrprhyk eyrrrkhtwd yedsqdgwys egydyhgtrl detriysdte
      421 yypstttsss rrraphrrps lerqmtlydd hsyytdgyss dgrvgkmsat ypecqtdiry
      481 neyydsitgy gyqdesrygq ddedrqwdsg gkwylgssty yenkrnrktl pqrpassigi
      541 hrpdyrptvt rqrsyeseel nysshstrrr kplqpqqrps srtegtgkkl pptpttpsnv
      601 vinrklaslp atpsrqlpkt rtpseesyyk sdynedynya yrsqdnlpqd tyidsiyseq
      661 sgydqmhvpg ktqyaedsqy gssytpqvdd hygrsyqepq tqdtydqtyl qnsykdeyqk
      721 pyveqntqvy qdtyqdmtyp nasqpndqyt sqpndqyrkt srdmiiddny qvmgfdknnv
      781 qyqdrtkvhp ykedtyqeqy edysvsvpss vydqnnvtnt ynqyqqdqyd sqynintstg
      841 yqksypdtyn qetyktevyd qdtynqgtyn qetykqdtyn qetykpdtyk edtykqdtyn
      901 qgtyiqdtyn qetynqgtyn qetypqdtyt qdtynqvpit sqsnyedays kpqkdyvdyq
      961 tpytkeetvt ttykneyeeq yqdypdqyee syhdsypgsf eerykdspsa sterrssevp
     1021 elsvttprgq trpngyqsse sdyyftsqes qdlsstglsr rkklekrdas plvqqntdsl
     1081 esrddelkes ietavssiss ipqkkvisdy stagdsspaa ptltespqst vqpsttmvnh
     1141 vtpnhvppsm tvtamvhptt tangkrltra esyqeevied eeypeiipke pqrkdsqlsh
     1201 qsqlsqhssq hsqhsqkpkl trgdsyasdh fpeesysrrs syaqptrkds fssttqesfk
     1261 ppltrtdsyq krgsygqnft apqpisraes yqrgyfkdqd sieepevgis navnddykmr
     1321 depleryera deamirgsre gslvdaykts ppmshtespr gsitkqasle esvqmdtppr
     1381 sppepppdee llemvgaapv ptqlkerpem tarqrwhway nqivmqlrng sgpggesggr
     1441 gnghlgdnpf ysnidsmpdi rprrksvplv seltmaatkr ntaglasavp ratlndeelk
     1501 mhvykktlqa miypissttp hkfvtwtats ptycyecegl lwgiarqgvr ctecgvkche
     1561 kckdllnadc lqraaekssk hgaedkansi itamkermkq rerekpeife lirsvfgvde
     1621 eahtrnmrav kqsvldgtsk wsakiaitvi caqgliakdk sgtsdpyvtv qvgkvkkrtr
     1681 tmprelnpvw hekfyfechn ssdrikvrvw dedndlkskl rqkltresdd flgqtiievr
     1741 tlsgemdvwy nlekrtdksa vsgairlhis veikgeekva pyhvqytclh enlfhslcek
     1801 ndgvvalpqa kgddawkvyf dppgeelvde famrygiesi yqamthfhcl stkylcpgvp
     1861 avmstllani nayyahtsas savsasdrfa asnfgkekfv klldqlhnsl ridlsmyrnn
     1921 fpassqeklm dlkstvdllt sitffrmkvq elsspprast vvkdcvkacl rstyqflfen
     1981 cydiynrefq vdpseakrda edhgpslesl dfwhkliali vsvieedkns yapvlnqfpq
     2041 elnigqlsaa tmwslfavdm kyaleeheqh rlckssaymn lhfkvkwlht nyvkdvppyk
     2101 gavpeypawf epfvmqwlne nddvsleylh gafnrdkkdg fqkssehalf svsvvdvftq
     2161 ltqcfdvvsk lecpdpeiwk rymkrfakti vkvlvayadi vkkefpshlk eeriacilmn
     2221 niqqlrvqle kmfesmggek leedaanilk elqqqlngal ddlamlfaks lepritasvk
     2281 elgdlllaik gggqvqlsqp aqrnnvavea devlrplmvl ldgslslyae scektvlkrl
     2341 lkelwkivmr ilektvvlpp msdksmmfkn ltdnaknlaa nakiedmskl fknhmsgkpd
     2401 vknalsgvmd iskeveknls pkqcavleva ldtikqyfha ggnglkkafl ekspelqslr
     2461 yalslytqtt dtliktfvts qvnqvplnka sqlrqrqrsm sserggdege gaegmeslee
     2521 plrqskpslr resrqvpdta gtdegsvgev siqvdlfthp gtgeqkvtvk vvaandlkwq
     2581 latgmfrpyv evnligphlt gkkrkqstks ksnnwspqfn esfdfmigne qqqldfyelh
     2641 icvkdycfar edrlvgvavm qlkdiveegs cacwlslgkr iqmdetgwti lrilsqrnnd
     2701 eiakefvklk sdirqetplp npt
//
LOCUS       XP_034195920             190 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611771 [Osmia lignaria lignaria].
ACCESSION   XP_034195920
VERSION     XP_034195920.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340029.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195920.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..190
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..190
                     /product="uncharacterized protein LOC117611771"
                     /calculated_mol_wt=21405
     CDS             1..190
                     /gene="LOC117611771"
                     /coded_by="XM_034340029.2:941..1513"
                     /db_xref="GeneID:117611771"
ORIGIN      
        1 miitrkvarw alllkrealg rnygaqkrse qapsgvtaml prsgrvddni dtdtdtvatd
       61 lhrtssrval alplpfpfpy yyhyyyhynp rvgiaasksl fkldrrtldk dheddlpipv
      121 eridepqgka tcakllaast epktkeqqdv ehgeqetenv prgiekeitv fasvdsncln
      181 yqnvrarfsa
//
LOCUS       XP_034195589            1160 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611661 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195589
VERSION     XP_034195589.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339698.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195589.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1160
                     /product="uncharacterized protein LOC117611661 isoform X1"
                     /calculated_mol_wt=129796
     Region          672..1014
                     /region_name="PKc_DYRK4"
                     /note="Catalytic domain of the protein kinase,
                     Dual-specificity tYrosine-phosphorylated and -Regulated
                     Kinase 4; cd14225"
                     /db_xref="CDD:271127"
     Site            order(722..726,730,743,745,759,781,797..800,803,805..806,
                     844,846,848..849,851,864,867,876,878..881,883,920)
                     /site_type="active"
                     /db_xref="CDD:271127"
     Site            order(722..726,730,743,745,781,797..800,803,844,846,
                     848..849,851,864)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271127"
     Site            order(759,805,844,846,867,876,878..881,883,920)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271127"
     Site            863..883
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271127"
     CDS             1..1160
                     /gene="LOC117611661"
                     /coded_by="XM_034339698.2:659..4141"
                     /db_xref="GeneID:117611661"
ORIGIN      
        1 mlgvsqpgnp psslrhsasf sclqrgtase ghrartstll qqpslqlsgs hhqyssgqts
       61 llqhstgtvq hriealesiq dsndygfvki rprlrstnat lyyeeevaaq knalwdrdas
      121 afsdrecdtn yrecslkmkd rdhspkvgss knqnplrgal ilftstssww karqredqln
      181 gstgdrtlgs ttsgvgssse rkwsstsmas psnerkwpsv tsptndrkws vgngaltspg
      241 nerkwtrssv csnstgqqdr kwrslgallr aptgngshav qhslshnmsv smiegehfrd
      301 dprgssckst ryqqptsysa rvsrvsrdiy rengefgqts grskvsrrly qdnnnaesie
      361 rdiddrhpnr hqfddecrgr tnresickte qtgnetrats trssraqsfy llddflrpqp
      421 qpnkcmlnvy lspshsksgn nndhgrstev kqisqgnvtn itpprrhnss sdslevatsp
      481 lpppvppppp qvttrdrerg enfkekdvcq ckmcctimhq rsfveevsrr skeraslyqe
      541 tgrspgtlhk dvggagtkvs gktvggigta tlttlssinn tsntsrnknn pvsqekllsf
      601 fhpkptrvek sdnnntnnss annihnnmpi hesinatshd vypgrlpmts qealkyygsr
      661 lteferaeie kyseiwylgl sahkihgeeg ssqnggydde ngsynkvlhd hisyryeile
      721 vigkgsfgqv irawdhktgh yiaikiirnk krfhhqalve veilehlrkk dldanashnv
      781 ihmleyfyfr nhlcitfelm slnlyelikk nnykgfslsl irrfanslis clrllyreki
      841 ihcdlkpenv llkqrgsssi kvidfgsscy shqrvytylq srfyrspevi lglpygtpid
      901 mwslgcilae lytgcplfpg edeieqlaci mevlglppeh iinhasrrrl ffdpkgnprc
      961 tsnskgkkrw vgsknlamal rctdtlfvdf vsrclewdpk krmtpdeamr hewlnssssh
     1021 vsssssstla tstvntnant nantnantts lettsqsaha qtisvtvsap rqrattvedp
     1081 pytmyrlykg rkyvqristt entdnsglvv ksklngsass halasstqtt tssrhastgd
     1141 ivasldpnld dsgtflppil
//
LOCUS       XP_034195591            1143 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611661 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195591
VERSION     XP_034195591.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339700.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195591.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1143
                     /product="uncharacterized protein LOC117611661 isoform X2"
                     /calculated_mol_wt=127791
     Region          655..997
                     /region_name="PKc_DYRK4"
                     /note="Catalytic domain of the protein kinase,
                     Dual-specificity tYrosine-phosphorylated and -Regulated
                     Kinase 4; cd14225"
                     /db_xref="CDD:271127"
     Site            order(705..709,713,726,728,742,764,780..783,786,788..789,
                     827,829,831..832,834,847,850,859,861..864,866,903)
                     /site_type="active"
                     /db_xref="CDD:271127"
     Site            order(705..709,713,726,728,764,780..783,786,827,829,
                     831..832,834,847)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271127"
     Site            order(742,788,827,829,850,859,861..864,866,903)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271127"
     Site            846..866
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271127"
     CDS             1..1143
                     /gene="LOC117611661"
                     /coded_by="XM_034339700.2:659..4090"
                     /db_xref="GeneID:117611661"
ORIGIN      
        1 mlgvsqpgnp psslrhsasf sclqrgtase ghrartstll qqpslqlsgs hhqyssgqts
       61 llqhstgtvq hriealesiq dsndygfvki rprlrstnat lyyeeevaaq knalwdrdas
      121 afsdrecdtn yrecslkmkd rdhspkvgss knqnplrgal ilftstssww karqredqln
      181 gstgdrtlgs ttsgvgssse rkwsstsmas psnerkwpsv tsptndrkws vgngaltspg
      241 nerkwtrssv csnstgqqdr kwrslgallr aptgngshav qhslshnmsv smiegehfrd
      301 dprgssckst ryqqptsysa rvsrvsrdiy rengefgqts grskvsrrly qdnnnaesie
      361 rdiddrhpnr hqfddecrgr tnresickte qtgnetrats trssraqsfy llddflrpqp
      421 qpnkcmlnvy lspshsksgn nndhgrstev kqisqgnvtn itpprrhnss sdslevatsp
      481 lpppvppppp qvttrdrerg enfkekdvcq ckmcctimhq rsfveespgt lhkdvggagt
      541 kvsgktvggi gtatlttlss inntsntsrn knnpvsqekl lsffhpkptr veksdnnntn
      601 nssannihnn mpihesinat shdvypgrlp mtsqealkyy gsrltefera eiekyseiwy
      661 lglsahkihg eegssqnggy ddengsynkv lhdhisyrye ilevigkgsf gqvirawdhk
      721 tghyiaikii rnkkrfhhqa lveveilehl rkkdldanas hnvihmleyf yfrnhlcitf
      781 elmslnlyel ikknnykgfs lslirrfans lisclrllyr ekiihcdlkp envllkqrgs
      841 ssikvidfgs scyshqrvyt ylqsrfyrsp evilglpygt pidmwslgci laelytgcpl
      901 fpgedeieql acimevlglp pehiinhasr rrlffdpkgn prctsnskgk krwvgsknla
      961 malrctdtlf vdfvsrclew dpkkrmtpde amrhewlnss sshvssssss tlatstvntn
     1021 antnantnan ttslettsqs ahaqtisvtv saprqrattv edppytmyrl ykgrkyvqri
     1081 sttentdnsg lvvksklngs asshalasst qtttssrhas tgdivasldp nlddsgtflp
     1141 pil
//
LOCUS       XP_076546742             109 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305773 [Osmia lignaria lignaria].
ACCESSION   XP_076546742
VERSION     XP_076546742.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690627.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..109
                     /product="uncharacterized protein LOC143305773"
                     /calculated_mol_wt=11803
     CDS             1..109
                     /gene="LOC143305773"
                     /coded_by="XM_076690627.1:25091..25420"
                     /db_xref="GeneID:143305773"
ORIGIN      
        1 mdalelqaal vkldpattvt pigssvkilp ahhriaftvd ldendltlek ntvqnndeks
       61 nnvsgvqttt taprkmqnnc saqltvashq nhpnkqqiev skcemaevr
//
LOCUS       XP_034195884            1459 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195884
VERSION     XP_034195884.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1459
                     /product="epidermal growth factor receptor isoform X1"
                     /calculated_mol_wt=163641
     Region          72..183
                     /region_name="Recep_L_domain"
                     /note="Receptor L domain; pfam01030"
                     /db_xref="CDD:460032"
     Region          201..346
                     /region_name="Furin-like"
                     /note="Furin-like cysteine rich region; pfam00757"
                     /db_xref="CDD:395614"
     Region          364..491
                     /region_name="Recep_L_domain"
                     /note="Receptor L domain; pfam01030"
                     /db_xref="CDD:460032"
     Region          516..629
                     /region_name="GF_recep_IV"
                     /note="Growth factor receptor domain IV; pfam14843"
                     /db_xref="CDD:464344"
     Region          684..>776
                     /region_name="GF_recep_IV"
                     /note="Growth factor receptor domain IV; pfam14843"
                     /db_xref="CDD:464344"
     Region          871..1149
                     /region_name="PTKc_EGFR_like"
                     /note="Catalytic domain of Epidermal Growth Factor
                     Receptor-like Protein Tyrosine Kinases; cd05057"
                     /db_xref="CDD:270648"
     Site            order(885..886,889..890,912,958,960,964,1008..1009,1022,
                     1043..1047,1052,1056)
                     /site_type="active"
                     /db_xref="CDD:270648"
     Site            order(885..886,893,910,912,933,957..958,960,1008..1009,
                     1011,1022)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270648"
     Site            1021..1046
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270648"
     Site            order(1043..1047,1052,1056)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270648"
     CDS             1..1459
                     /gene="Egfr"
                     /coded_by="XM_034339993.2:1120..5499"
                     /db_xref="GeneID:117611766"
ORIGIN      
        1 mfqhrhgprt lstcnlfgvt fllliiagsy dvsadlnsef vkgkicigtn grlsvpsnkq
       61 hhyrnlrdry tnctyvdgnl eitwlqnetf dlsflqyire vtgyvlishv dvrkivlprl
      121 qiirgrtlfk lnihaeefal fvtmcqmqnl empalrdiln gsvgmynnyn lchirtinwd
      181 eiitsqsaty iyvynftspe rvcspcdksc eqgcwgegpe ncqkysktnc spqcwqgrcf
      241 gpnprecchl fcaggctgpk qsdciacknf fddgvctqec ppmqkynptt ysweanpdgk
      301 yaygatcvrr cpehllkdng acvrscppkk kalngecvpc dgpcpktckg vekvhsgnid
      361 sfkdctiieg sitildqsfq gfqhvypnfs fgkryekmhp dklevfstlk eitgflniqg
      421 dhkdfknlsy frnleviggr tlteyfasly ivktslvsfg lsslkkiysg siailenknl
      481 cyaqsinwtr ikkssehesl lsnnrnesec ikdglvcdeq cseegcwgpg paqclscknf
      541 ilgndclqdc tapgiyqade ktcklchdec dgsctgpnae hctkckhard gpfcvpecps
      601 skyndngqck dchencvggc egpennigpn gchscdkaim nghvpegclq krepcpdghy
      661 yewvspqeqg alkplagkav crechprckk ctgygfhehv cqecmkykkg eqcedecpad
      721 hfadadtqrc ipcfsecrgc fgpepnqcyk crnykihide didgntttfn ctetcppeyp
      781 hkifppdsep ycsletvglg fqvenelqpa ilagvavfal vflvvaaiim yfwrvrvkak
      841 enavkmtmtl tglddneplr ptgvkpnlak lriikeeemr kggilgygaf gnvykgvwvp
      901 egenvkipva ikvlhdgtga ntskefldea yimasvehpn llqllavcmt sqmmlvtqlm
      961 plgclldfvr rfkdkigske llnwctqiar gmayleerrl vhrdlaarnv lvqtsncvki
     1021 tdfglaklld ineeqykaag gkmpikwlal eciqhrvfth ksdvwafgvt iwevltyggr
     1081 pyenvparnv pellekgerl pqpsictidv ymimikcwml daesrpsfke laedfakmsr
     1141 dpgrylaikg dkymrlpsyt lqdekemirn lasamdgpea lvdaeeylqp ksrapippgl
     1201 sasstsgspp ntpvktcwpn gkplaadspt pqnqqnwdre llrygtnhrn gntshepgns
     1261 gqhphyappn ghcghvvgsd tsssrycsdp lkmigvrdcd vtddcfdgev nsahqqaqvg
     1321 nlkldlplde ddylmpspql ptnttqymdl igdsktteme pkrvnngyrk ypefltiqgk
     1381 tsldnpeyim sqdegpltpq tigiptpdle kvltngtfgs qvrqrsseee sdheyyndfd
     1441 rlerelqplk plrknettv
//
LOCUS       XP_034195883            1459 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195883
VERSION     XP_034195883.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339992.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1459
                     /product="epidermal growth factor receptor isoform X1"
                     /calculated_mol_wt=163641
     Region          72..183
                     /region_name="Recep_L_domain"
                     /note="Receptor L domain; pfam01030"
                     /db_xref="CDD:460032"
     Region          201..346
                     /region_name="Furin-like"
                     /note="Furin-like cysteine rich region; pfam00757"
                     /db_xref="CDD:395614"
     Region          364..491
                     /region_name="Recep_L_domain"
                     /note="Receptor L domain; pfam01030"
                     /db_xref="CDD:460032"
     Region          516..629
                     /region_name="GF_recep_IV"
                     /note="Growth factor receptor domain IV; pfam14843"
                     /db_xref="CDD:464344"
     Region          684..>776
                     /region_name="GF_recep_IV"
                     /note="Growth factor receptor domain IV; pfam14843"
                     /db_xref="CDD:464344"
     Region          871..1149
                     /region_name="PTKc_EGFR_like"
                     /note="Catalytic domain of Epidermal Growth Factor
                     Receptor-like Protein Tyrosine Kinases; cd05057"
                     /db_xref="CDD:270648"
     Site            order(885..886,889..890,912,958,960,964,1008..1009,1022,
                     1043..1047,1052,1056)
                     /site_type="active"
                     /db_xref="CDD:270648"
     Site            order(885..886,893,910,912,933,957..958,960,1008..1009,
                     1011,1022)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270648"
     Site            1021..1046
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270648"
     Site            order(1043..1047,1052,1056)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270648"
     CDS             1..1459
                     /gene="Egfr"
                     /coded_by="XM_034339992.2:1028..5407"
                     /db_xref="GeneID:117611766"
ORIGIN      
        1 mfqhrhgprt lstcnlfgvt fllliiagsy dvsadlnsef vkgkicigtn grlsvpsnkq
       61 hhyrnlrdry tnctyvdgnl eitwlqnetf dlsflqyire vtgyvlishv dvrkivlprl
      121 qiirgrtlfk lnihaeefal fvtmcqmqnl empalrdiln gsvgmynnyn lchirtinwd
      181 eiitsqsaty iyvynftspe rvcspcdksc eqgcwgegpe ncqkysktnc spqcwqgrcf
      241 gpnprecchl fcaggctgpk qsdciacknf fddgvctqec ppmqkynptt ysweanpdgk
      301 yaygatcvrr cpehllkdng acvrscppkk kalngecvpc dgpcpktckg vekvhsgnid
      361 sfkdctiieg sitildqsfq gfqhvypnfs fgkryekmhp dklevfstlk eitgflniqg
      421 dhkdfknlsy frnleviggr tlteyfasly ivktslvsfg lsslkkiysg siailenknl
      481 cyaqsinwtr ikkssehesl lsnnrnesec ikdglvcdeq cseegcwgpg paqclscknf
      541 ilgndclqdc tapgiyqade ktcklchdec dgsctgpnae hctkckhard gpfcvpecps
      601 skyndngqck dchencvggc egpennigpn gchscdkaim nghvpegclq krepcpdghy
      661 yewvspqeqg alkplagkav crechprckk ctgygfhehv cqecmkykkg eqcedecpad
      721 hfadadtqrc ipcfsecrgc fgpepnqcyk crnykihide didgntttfn ctetcppeyp
      781 hkifppdsep ycsletvglg fqvenelqpa ilagvavfal vflvvaaiim yfwrvrvkak
      841 enavkmtmtl tglddneplr ptgvkpnlak lriikeeemr kggilgygaf gnvykgvwvp
      901 egenvkipva ikvlhdgtga ntskefldea yimasvehpn llqllavcmt sqmmlvtqlm
      961 plgclldfvr rfkdkigske llnwctqiar gmayleerrl vhrdlaarnv lvqtsncvki
     1021 tdfglaklld ineeqykaag gkmpikwlal eciqhrvfth ksdvwafgvt iwevltyggr
     1081 pyenvparnv pellekgerl pqpsictidv ymimikcwml daesrpsfke laedfakmsr
     1141 dpgrylaikg dkymrlpsyt lqdekemirn lasamdgpea lvdaeeylqp ksrapippgl
     1201 sasstsgspp ntpvktcwpn gkplaadspt pqnqqnwdre llrygtnhrn gntshepgns
     1261 gqhphyappn ghcghvvgsd tsssrycsdp lkmigvrdcd vtddcfdgev nsahqqaqvg
     1321 nlkldlplde ddylmpspql ptnttqymdl igdsktteme pkrvnngyrk ypefltiqgk
     1381 tsldnpeyim sqdegpltpq tigiptpdle kvltngtfgs qvrqrsseee sdheyyndfd
     1441 rlerelqplk plrknettv
//
LOCUS       XP_034195885            1442 aa            linear   INV 03-SEP-2025
DEFINITION  epidermal growth factor receptor isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195885
VERSION     XP_034195885.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1442
                     /product="epidermal growth factor receptor isoform X2"
                     /calculated_mol_wt=161832
     Region          55..166
                     /region_name="Recep_L_domain"
                     /note="Receptor L domain; pfam01030"
                     /db_xref="CDD:460032"
     Region          184..329
                     /region_name="Furin-like"
                     /note="Furin-like cysteine rich region; pfam00757"
                     /db_xref="CDD:395614"
     Region          347..474
                     /region_name="Recep_L_domain"
                     /note="Receptor L domain; pfam01030"
                     /db_xref="CDD:460032"
     Region          499..612
                     /region_name="GF_recep_IV"
                     /note="Growth factor receptor domain IV; pfam14843"
                     /db_xref="CDD:464344"
     Region          667..>759
                     /region_name="GF_recep_IV"
                     /note="Growth factor receptor domain IV; pfam14843"
                     /db_xref="CDD:464344"
     Region          854..1132
                     /region_name="PTKc_EGFR_like"
                     /note="Catalytic domain of Epidermal Growth Factor
                     Receptor-like Protein Tyrosine Kinases; cd05057"
                     /db_xref="CDD:270648"
     Site            order(868..869,872..873,895,941,943,947,991..992,1005,
                     1026..1030,1035,1039)
                     /site_type="active"
                     /db_xref="CDD:270648"
     Site            order(868..869,876,893,895,916,940..941,943,991..992,994,
                     1005)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270648"
     Site            1004..1029
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270648"
     Site            order(1026..1030,1035,1039)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270648"
     CDS             1..1442
                     /gene="Egfr"
                     /coded_by="XM_034339994.2:668..4996"
                     /db_xref="GeneID:117611766"
ORIGIN      
        1 mgpakilgvy vlfmfvyigk tdvieervci gtngrlsvps nkqhhyrnlr drytnctyvd
       61 gnleitwlqn etfdlsflqy irevtgyvli shvdvrkivl prlqiirgrt lfklnihaee
      121 falfvtmcqm qnlempalrd ilngsvgmyn nynlchirti nwdeiitsqs atyiyvynft
      181 spervcspcd ksceqgcwge gpencqkysk tncspqcwqg rcfgpnprec chlfcaggct
      241 gpkqsdciac knffddgvct qecppmqkyn pttysweanp dgkyaygatc vrrcpehllk
      301 dngacvrscp pkkkalngec vpcdgpcpkt ckgvekvhsg nidsfkdcti iegsitildq
      361 sfqgfqhvyp nfsfgkryek mhpdklevfs tlkeitgfln iqgdhkdfkn lsyfrnlevi
      421 ggrtlteyfa slyivktslv sfglsslkki ysgsiailen knlcyaqsin wtrikksseh
      481 esllsnnrne secikdglvc deqcseegcw gpgpaqclsc knfilgndcl qdctapgiyq
      541 adektcklch decdgsctgp naehctkckh ardgpfcvpe cpsskyndng qckdchencv
      601 ggcegpenni gpngchscdk aimnghvpeg clqkrepcpd ghyyewvspq eqgalkplag
      661 kavcrechpr ckkctgygfh ehvcqecmky kkgeqcedec padhfadadt qrcipcfsec
      721 rgcfgpepnq cykcrnykih idedidgntt tfnctetcpp eyphkifppd sepycsletv
      781 glgfqvenel qpailagvav falvflvvaa iimyfwrvrv kakenavkmt mtltglddne
      841 plrptgvkpn laklriikee emrkggilgy gafgnvykgv wvpegenvki pvaikvlhdg
      901 tgantskefl deayimasve hpnllqllav cmtsqmmlvt qlmplgclld fvrrfkdkig
      961 skellnwctq iargmaylee rrlvhrdlaa rnvlvqtsnc vkitdfglak lldineeqyk
     1021 aaggkmpikw laleciqhrv fthksdvwaf gvtiwevlty ggrpyenvpa rnvpellekg
     1081 erlpqpsict idvymimikc wmldaesrps fkelaedfak msrdpgryla ikgdkymrlp
     1141 sytlqdekem irnlasamdg pealvdaeey lqpksrapip pglsasstsg sppntpvktc
     1201 wpngkplaad sptpqnqqnw drellrygtn hrngntshep gnsgqhphya ppnghcghvv
     1261 gsdtsssryc sdplkmigvr dcdvtddcfd gevnsahqqa qvgnlkldlp ldeddylmps
     1321 pqlptnttqy mdligdsktt emepkrvnng yrkypeflti qgktsldnpe yimsqdegpl
     1381 tpqtigiptp dlekvltngt fgsqvrqrss eeesdheyyn dfdrlerelq plkplrknet
     1441 tv
//
LOCUS       XP_034195888             462 aa            linear   INV 03-SEP-2025
DEFINITION  2-aminoadipate transaminase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195888
VERSION     XP_034195888.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339997.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195888.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="2-aminoadipate transaminase isoform X1"
                     /calculated_mol_wt=52770
     Region          30..450
                     /region_name="ARO8"
                     /note="DNA-binding transcriptional regulator, MocR family,
                     contains an aminotransferase domain [Transcription, Amino
                     acid transport and metabolism]; COG1167"
                     /db_xref="CDD:440781"
     CDS             1..462
                     /gene="LOC117611767"
                     /coded_by="XM_034339997.2:367..1755"
                     /db_xref="GeneID:117611767"
ORIGIN      
        1 mnetvgeitq slvtafphfs qllsvvrphy ytahnmettl ydpylrhlfd gdpvfnvyne
       61 eatnlsvgap gpdllqhcve miqkatdhrl eeekkegkyy lfqygitagl wecrdelaif
      121 lnrrygdpvk renliltcga shglqlllnt vlspngiifv eevtymiald afkqfplmki
      181 vtvpmkndiv dldmlekiiv eekrnsnfll neekifwamf ytiptfhnpt gttlppesck
      241 rlieiarsns ivvacddvyn llyygdnfpp hrlffydnpt dpnykggnvv sngsfskils
      301 pairfgwiec sprvakifrs sgilcsggav nhyvsgvias llhlkiqdeh ldrlvqvyre
      361 rlstlcnvld eylpkqcsyq rpdggyfvwi hlppeldgsd fikwcqeehk vsaipgvrfs
      421 ykkncpnflr isigfhkkev lenaskilck gllqyikeni ks
//
LOCUS       XP_034195889             427 aa            linear   INV 03-SEP-2025
DEFINITION  2-aminoadipate transaminase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195889
VERSION     XP_034195889.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339998.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195889.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="2-aminoadipate transaminase isoform X2"
                     /calculated_mol_wt=48799
     Region          <29..415
                     /region_name="ARO8"
                     /note="DNA-binding transcriptional regulator, MocR family,
                     contains an aminotransferase domain [Transcription, Amino
                     acid transport and metabolism]; COG1167"
                     /db_xref="CDD:440781"
     CDS             1..427
                     /gene="LOC117611767"
                     /coded_by="XM_034339998.2:586..1869"
                     /db_xref="GeneID:117611767"
ORIGIN      
        1 mettlydpyl rhlfdgdpvf nvyneeatnl svgapgpdll qhcvemiqka tdhrleeekk
       61 egkyylfqyg itaglwecrd elaiflnrry gdpvkrenli ltcgashglq lllntvlspn
      121 giifveevty mialdafkqf plmkivtvpm kndivdldml ekiiveekrn snfllneeki
      181 fwamfytipt fhnptgttlp pesckrliei arsnsivvac ddvynllyyg dnfpphrlff
      241 ydnptdpnyk ggnvvsngsf skilspairf gwiecsprva kifrssgilc sggavnhyvs
      301 gviasllhlk iqdehldrlv qvyrerlstl cnvldeylpk qcsyqrpdgg yfvwihlppe
      361 ldgsdfikwc qeehkvsaip gvrfsykknc pnflrisigf hkkevlenas kilckgllqy
      421 ikeniks
//
LOCUS       XP_034195890             268 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosome recycling factor 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195890
VERSION     XP_034195890.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339999.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195890.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="mitochondrial ribosome recycling factor 1"
                     /calculated_mol_wt=30992
     Site            order(114..117,187..190)
                     /site_type="other"
                     /note="hinge region"
                     /db_xref="CDD:238288"
     Region          116..264
                     /region_name="RRF"
                     /note="Ribosome recycling factor; pfam01765"
                     /db_xref="CDD:460316"
     CDS             1..268
                     /gene="mRRF1"
                     /coded_by="XM_034339999.2:201..1007"
                     /db_xref="GeneID:117611768"
ORIGIN      
        1 mhstllrhql leltncltrh vrslyilkgy enilisnelm sskfnsycyt fnlnkvnqfs
       61 ttsmclknks rvkdkskvhv dvhqlaevik lnkmmsefde eleklkedli kyanvrsnig
      121 aienlptmye deeyklaelv eisrkpnmiv lniaafpqii psiletlaks qmnlnpqqeg
      181 ttiyvpipki tkehrenlak nakqyfvkck taisdirnly vkklkraesv pedliyngen
      241 yitavqreyv ekaeqllktk qkellges
//
LOCUS       XP_034194872             824 aa            linear   INV 03-SEP-2025
DEFINITION  ribonucleoside diphosphate reductase large subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034194872
VERSION     XP_034194872.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338981.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194872.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..824
                     /product="ribonucleoside diphosphate reductase large
                     subunit"
                     /calculated_mol_wt=93704
     Region          7..824
                     /region_name="PLN02437"
                     /note="ribonucleoside--diphosphate reductase large
                     subunit"
                     /db_xref="CDD:178056"
     Site            order(208..209,223..224,253,433,435,437,452,609..614)
                     /site_type="active"
                     /db_xref="CDD:153088"
     Site            order(220,234..235,238,241..242,245..246,249,264,276,
                     280..281,283..284,287..288,291..295)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153088"
     Site            order(224,433,435,437,450,744..745)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:153088"
     Site            order(232..234,237,249,262,276,281)
                     /site_type="active"
                     /note="effector binding site [active]"
                     /db_xref="CDD:153088"
     Site            order(342,345..346,348..349,393,397,404,723..725,727..728,
                     732..733,735..736)
                     /site_type="active"
                     /note="R2 peptide binding site [active]"
                     /db_xref="CDD:153088"
     CDS             1..824
                     /gene="RnrL"
                     /coded_by="XM_034338981.2:118..2592"
                     /db_xref="GeneID:117611084"
ORIGIN      
        1 mvgkgkmfvv krdgrkedvh fdkitsriqk lcynldmdyv dpsaitirvi nglysgvstv
       61 eldnlaaeta atmttkhpdy avlaariavs nlhketkksf sdvihdlyhv keqytnkpkp
      121 visekyynii qnnanklnsa iiydrdfsyn yfgfktlers yllkihgkvv erpqqmlmrv
      181 avaihredid raietynfls eryfthaspt lfsactmvqq msscflltms edsiegiydt
      241 lkrcalisks aggiglnvhc irargtpiag tlgvsnglvp mlrvynntar yvdqggnkrp
      301 gafaiylepw hadifefldl kkntgkeenr arelfyalwi pdlfmkrvla ndvwtlmcph
      361 espgladiwg defealytry enekrykrqv qardlwtail vaqvetgtpy mlykdhcnrk
      421 snqqnigtik nsnlcteivq yssadevavc nlasiavnmf vnstnkrfdf qklkkvtkiv
      481 trnldkvidi nyypipeakt snlkhrpigi giqgladafl llrypfesee aqklniqife
      541 tlyygaleas celaeekgky etyegspisk gilqydmwdv tptdlwdwaa lkekiakhgv
      601 rnslliapmp tastaqilgn nesiepytsn iytrrvlsge fqivnphllr dlterdlwde
      661 dmkneiiann gsiqnverip telkmlyktv weipqktvlk maadrgafid qsqslnvhma
      721 kpstekltsm hfygwqmglk tgmyylrtkp aanalqftvd ksklrnvstt taneslnsen
      781 enneeenkfd tsktswiqnq stenieavls csrengdacm acgs
//
LOCUS       XP_034196155             806 aa            linear   INV 03-SEP-2025
DEFINITION  trehalose-6-phosphate synthase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196155
VERSION     XP_034196155.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340264.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..806
                     /product="trehalose-6-phosphate synthase 1 isoform X1"
                     /calculated_mol_wt=90763
     Region          15..763
                     /region_name="PRK14501"
                     /note="putative bifunctional trehalose-6-phosphate
                     synthase/HAD hydrolase subfamily IIB; Provisional"
                     /db_xref="CDD:184712"
     CDS             1..806
                     /gene="Tps1"
                     /coded_by="XM_034340264.2:28..2448"
                     /db_xref="GeneID:117611863"
ORIGIN      
        1 mstepgsfts ngsmivvsnr lpfvlkrnev tgklerkasa gglvtavapv visgngvwvg
       61 wpgmhmenrn epipesdpnd rtptagllsr kvvavhvdpt ifdsyyngcc ngtfwplfhs
      121 mpdratfiad hwraystvne efaaktvgal eqihkeqenq sngtplvwvh dyhlmltanw
      181 irqaaeekrl klklgfflhi pfppwdifrl fpwadeilqg mlgcdmvgfh iqdyclnfvd
      241 ccqrslgcrv drknllvehg grtvrvrplp igipfdrfvs laetankvms tnqkivlgvd
      301 rldytkglvh rlkafemlle khpehreqvt mlqiavpsrt dvreyqdlkl emdqligrin
      361 grfttpnwsp iryiygcvsq delaafyrda avalvtplrd gmnlvakefv acqintppgv
      421 livspfagag emmhealicn pyeideaaev ihraltmped ertlrmnhlr rrerlydvny
      481 wmksflqvmg sleehdsvga ttmqpvtmdd fddylskspr yigdnhklal lldydgtlap
      541 iathpdlail pletknvlqr lsnmsdvyia iisgrnvnnv ksmvgiegit yagnhgleil
      601 hpdgskfvhp mpaelegkva nlmqalqdql crdgawvenk galltfhyre tpmegrtemi
      661 eqakkiiena gfkpcpahca ieakppvewn kgrasiyilr tafgldwser iriiyagddv
      721 tdedamkalk gmaatfrvas shiirtsaer rlpstdsvlt mlkwverhls krkprnnadi
      781 psrfrrgsag itmemsymps natles
//
LOCUS       XP_034196156             803 aa            linear   INV 03-SEP-2025
DEFINITION  trehalose-6-phosphate synthase 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196156
VERSION     XP_034196156.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340265.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..803
                     /product="trehalose-6-phosphate synthase 1 isoform X2"
                     /calculated_mol_wt=90422
     Region          15..760
                     /region_name="PRK14501"
                     /note="putative bifunctional trehalose-6-phosphate
                     synthase/HAD hydrolase subfamily IIB; Provisional"
                     /db_xref="CDD:184712"
     CDS             1..803
                     /gene="Tps1"
                     /coded_by="XM_034340265.2:28..2439"
                     /db_xref="GeneID:117611863"
ORIGIN      
        1 mstepgsfts ngsmivvsnr lpfvlkrnev tgklerkasa gglvtavapv visgngvwvg
       61 wpgmhmenrn epipesdpnd rtptagllsr kvvavhvdpt ifdsyyngcc ngtfwplfhs
      121 mpdratfiad hwraystvne efaaktvgal eqihkeqenq sngtplvwvh dyhlmltanw
      181 irqaaeekrl klklgfflhi pfppwdifrl fpwadeilqg mlgcdmvgfh iqdyclnfvd
      241 ccqrslgcrv drknllvehg grtvrvrplp igipfdrfvs laetankvms tnqkivlgvd
      301 rldytkglvh rlkafemlle khpehreqvt mlqiavpsrt dvreyqdlkl emdqligrin
      361 grfttpnwsp iryiygcvsq delaafyrda avalvtplrd gmnlvakefv acqintppgv
      421 livspfagag emmhealicn pyeideaaev ihraltmped ertlrmnhlr rrerlydvny
      481 wmksflqvmg sleehdsvga ttmqpvtmdd fddylskyig dnhklallld ydgtlapiat
      541 hpdlailple tknvlqrlsn msdvyiaiis grnvnnvksm vgiegityag nhgleilhpd
      601 gskfvhpmpa elegkvanlm qalqdqlcrd gawvenkgal ltfhyretpm egrtemieqa
      661 kkiienagfk pcpahcaiea kppvewnkgr asiyilrtaf gldwseriri iyagddvtde
      721 damkalkgma atfrvasshi irtsaerrlp stdsvltmlk wverhlskrk prnnadipsr
      781 frrgsagitm emsympsnat les
//
LOCUS       XP_034194879             646 aa            linear   INV 03-SEP-2025
DEFINITION  GABA neurotransmitter transporter-1B [Osmia lignaria lignaria].
ACCESSION   XP_034194879
VERSION     XP_034194879.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="GABA neurotransmitter transporter-1B"
                     /calculated_mol_wt=72043
     Region          72..621
                     /region_name="SLC6sbd-TauT-like"
                     /note="Na(+)- and Cl(-)-dependent taurine transporter
                     TauT, and related proteins; solute-binding domain;
                     cd11496"
                     /db_xref="CDD:271387"
     Site            order(87,90,440,443..444)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(88..89,91..94,168,342..343,348,350,444,447..448)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     Site            order(89,94,343,375)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(96..97,167,171,174,409,413)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     CDS             1..646
                     /gene="Gat-1B"
                     /coded_by="XM_034338988.2:313..2253"
                     /db_xref="GeneID:117611089"
ORIGIN      
        1 mpssgsarvp kvdrstsplp hkpanpvqel kalfagpssr vsngskeqdf rfeaiqclqs
       61 svtkesktlp ergtwsskve filsviglai glgnlwrfpy lcykngggaf mvpyfialal
      121 agvpmflmel slgqmmtigg lgvfkiapif kgigyatcvl scwtnvyyii ilawalfyfl
      181 vslridvpwr tcgnpwnsry cltsterlea lcwpengdti cstsignlsh sllkdpvkef
      241 werrtlqisd gvenvgsirw elagtlaivw imcyfciwkg vkwtgkvvyf tslfpyalla
      301 vllvrgltlp gameglryya tpnlsklgdp evwidavtqi ffsyalglga lvalgsynkf
      361 nnnvykdali vctvnsctsm lsgvvifsvv gfmaheqqkp vadvaasgpg laflvypsav
      421 lelpgssiwa clfffmlili gldsqfctve gfitaavdew pqllrkrkei fiaivcfisy
      481 lvglscvteg gmyvfqllds yavsgfcllf lmffecisvs wafgvnrfyd girdmigyyp
      541 cywwkicwtv ttpavcvgvf tfniikfvpv kyltyeypww shllgwlcgl ssmlcipgym
      601 iyiwcttsgt tsekfrklvr idddvatlrm klnptkaaai ntefel
//
LOCUS       XP_076546743             646 aa            linear   INV 03-SEP-2025
DEFINITION  GABA neurotransmitter transporter-1B [Osmia lignaria lignaria].
ACCESSION   XP_076546743
VERSION     XP_076546743.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690628.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="GABA neurotransmitter transporter-1B"
                     /calculated_mol_wt=72043
     Region          72..621
                     /region_name="SLC6sbd-TauT-like"
                     /note="Na(+)- and Cl(-)-dependent taurine transporter
                     TauT, and related proteins; solute-binding domain;
                     cd11496"
                     /db_xref="CDD:271387"
     Site            order(87,90,440,443..444)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(88..89,91..94,168,342..343,348,350,444,447..448)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     Site            order(89,94,343,375)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(96..97,167,171,174,409,413)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     CDS             1..646
                     /gene="Gat-1B"
                     /coded_by="XM_076690628.1:764..2704"
                     /db_xref="GeneID:117611089"
ORIGIN      
        1 mpssgsarvp kvdrstsplp hkpanpvqel kalfagpssr vsngskeqdf rfeaiqclqs
       61 svtkesktlp ergtwsskve filsviglai glgnlwrfpy lcykngggaf mvpyfialal
      121 agvpmflmel slgqmmtigg lgvfkiapif kgigyatcvl scwtnvyyii ilawalfyfl
      181 vslridvpwr tcgnpwnsry cltsterlea lcwpengdti cstsignlsh sllkdpvkef
      241 werrtlqisd gvenvgsirw elagtlaivw imcyfciwkg vkwtgkvvyf tslfpyalla
      301 vllvrgltlp gameglryya tpnlsklgdp evwidavtqi ffsyalglga lvalgsynkf
      361 nnnvykdali vctvnsctsm lsgvvifsvv gfmaheqqkp vadvaasgpg laflvypsav
      421 lelpgssiwa clfffmlili gldsqfctve gfitaavdew pqllrkrkei fiaivcfisy
      481 lvglscvteg gmyvfqllds yavsgfcllf lmffecisvs wafgvnrfyd girdmigyyp
      541 cywwkicwtv ttpavcvgvf tfniikfvpv kyltyeypww shllgwlcgl ssmlcipgym
      601 iyiwcttsgt tsekfrklvr idddvatlrm klnptkaaai ntefel
//
LOCUS       XP_076546744             646 aa            linear   INV 03-SEP-2025
DEFINITION  GABA neurotransmitter transporter-1B [Osmia lignaria lignaria].
ACCESSION   XP_076546744
VERSION     XP_076546744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690629.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="GABA neurotransmitter transporter-1B"
                     /calculated_mol_wt=72043
     Region          72..621
                     /region_name="SLC6sbd-TauT-like"
                     /note="Na(+)- and Cl(-)-dependent taurine transporter
                     TauT, and related proteins; solute-binding domain;
                     cd11496"
                     /db_xref="CDD:271387"
     Site            order(87,90,440,443..444)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(88..89,91..94,168,342..343,348,350,444,447..448)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     Site            order(89,94,343,375)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(96..97,167,171,174,409,413)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     CDS             1..646
                     /gene="Gat-1B"
                     /coded_by="XM_076690629.1:826..2766"
                     /db_xref="GeneID:117611089"
ORIGIN      
        1 mpssgsarvp kvdrstsplp hkpanpvqel kalfagpssr vsngskeqdf rfeaiqclqs
       61 svtkesktlp ergtwsskve filsviglai glgnlwrfpy lcykngggaf mvpyfialal
      121 agvpmflmel slgqmmtigg lgvfkiapif kgigyatcvl scwtnvyyii ilawalfyfl
      181 vslridvpwr tcgnpwnsry cltsterlea lcwpengdti cstsignlsh sllkdpvkef
      241 werrtlqisd gvenvgsirw elagtlaivw imcyfciwkg vkwtgkvvyf tslfpyalla
      301 vllvrgltlp gameglryya tpnlsklgdp evwidavtqi ffsyalglga lvalgsynkf
      361 nnnvykdali vctvnsctsm lsgvvifsvv gfmaheqqkp vadvaasgpg laflvypsav
      421 lelpgssiwa clfffmlili gldsqfctve gfitaavdew pqllrkrkei fiaivcfisy
      481 lvglscvteg gmyvfqllds yavsgfcllf lmffecisvs wafgvnrfyd girdmigyyp
      541 cywwkicwtv ttpavcvgvf tfniikfvpv kyltyeypww shllgwlcgl ssmlcipgym
      601 iyiwcttsgt tsekfrklvr idddvatlrm klnptkaaai ntefel
//
LOCUS       XP_076546745             646 aa            linear   INV 03-SEP-2025
DEFINITION  GABA neurotransmitter transporter-1B [Osmia lignaria lignaria].
ACCESSION   XP_076546745
VERSION     XP_076546745.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690630.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="GABA neurotransmitter transporter-1B"
                     /calculated_mol_wt=72043
     Region          72..621
                     /region_name="SLC6sbd-TauT-like"
                     /note="Na(+)- and Cl(-)-dependent taurine transporter
                     TauT, and related proteins; solute-binding domain;
                     cd11496"
                     /db_xref="CDD:271387"
     Site            order(87,90,440,443..444)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(88..89,91..94,168,342..343,348,350,444,447..448)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     Site            order(89,94,343,375)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(96..97,167,171,174,409,413)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     CDS             1..646
                     /gene="Gat-1B"
                     /coded_by="XM_076690630.1:210..2150"
                     /db_xref="GeneID:117611089"
ORIGIN      
        1 mpssgsarvp kvdrstsplp hkpanpvqel kalfagpssr vsngskeqdf rfeaiqclqs
       61 svtkesktlp ergtwsskve filsviglai glgnlwrfpy lcykngggaf mvpyfialal
      121 agvpmflmel slgqmmtigg lgvfkiapif kgigyatcvl scwtnvyyii ilawalfyfl
      181 vslridvpwr tcgnpwnsry cltsterlea lcwpengdti cstsignlsh sllkdpvkef
      241 werrtlqisd gvenvgsirw elagtlaivw imcyfciwkg vkwtgkvvyf tslfpyalla
      301 vllvrgltlp gameglryya tpnlsklgdp evwidavtqi ffsyalglga lvalgsynkf
      361 nnnvykdali vctvnsctsm lsgvvifsvv gfmaheqqkp vadvaasgpg laflvypsav
      421 lelpgssiwa clfffmlili gldsqfctve gfitaavdew pqllrkrkei fiaivcfisy
      481 lvglscvteg gmyvfqllds yavsgfcllf lmffecisvs wafgvnrfyd girdmigyyp
      541 cywwkicwtv ttpavcvgvf tfniikfvpv kyltyeypww shllgwlcgl ssmlcipgym
      601 iyiwcttsgt tsekfrklvr idddvatlrm klnptkaaai ntefel
//
LOCUS       XP_034196161             354 aa            linear   INV 03-SEP-2025
DEFINITION  uridylate-specific endoribonuclease B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196161
VERSION     XP_034196161.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340270.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..354
                     /product="uridylate-specific endoribonuclease B isoform
                     X1"
                     /calculated_mol_wt=40872
     Region          88..349
                     /region_name="XendoU"
                     /note="Xenopus laevis endoribonuclease XendoU, and related
                     proteins; cd21159"
                     /db_xref="CDD:394910"
     Site            order(218,220,227,232,236,246,248,291,334,336,346,348)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:394910"
     CDS             1..354
                     /gene="LOC117611866"
                     /coded_by="XM_034340270.2:248..1312"
                     /db_xref="GeneID:117611866"
ORIGIN      
        1 mygivillls msiqllcnli glnstgynlq hiyticlklk hfnikkmlsk iigvsfyilv
       61 ilntvflyis tsfqigeasi lpnmslisni elinlseqif nkssyelfrh liiryqgqtn
      121 lknftdvape rllyvsprip wtettksfmk lldnyeldty qpeiitkded ieenefinnl
      181 mkadvilhvm nflsvkgffe nnsliyknil keiwfhlysr skgingssgf ehvfvgerkp
      241 rkgitglhnw isfssgelln tinyfgfshn melsdkmgil etyftygnkr kmstifigtt
      301 pelevalytl cffarpnkrc nvsftkfkfs iqtyvlhsnn kkfvasafpi igkr
//
LOCUS       XP_034196164             308 aa            linear   INV 03-SEP-2025
DEFINITION  endoribonuclease Arlr isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196164
VERSION     XP_034196164.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340273.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="endoribonuclease Arlr isoform X2"
                     /calculated_mol_wt=35482
     Region          88..303
                     /region_name="XendoU"
                     /note="Xenopus laevis endoribonuclease XendoU, and related
                     proteins; cd21159"
                     /db_xref="CDD:394910"
     CDS             1..308
                     /gene="LOC117611866"
                     /coded_by="XM_034340273.2:254..1180"
                     /db_xref="GeneID:117611866"
ORIGIN      
        1 mygivillls msiqllcnli glnstgynlq hiyticlklk hfnikkmlsk iigvsfyilv
       61 ilntvflyis tsfqigeasi lpnmslisni elinlseqif nkssyelfrh liiryqgqtn
      121 lknftdvape ripwtettks fmklldnyel dtyqpeiitk dedieenefi nnlmkadvil
      181 hvmnflsvkg erkprkgitg lhnwisfssg ellntinyfg fshnmelsdk mgiletyfty
      241 gnkrkmstif igttpeleva lytlcffarp nkrcnvsftk fkfsiqtyvl hsnnkkfvas
      301 afpiigkr
//
LOCUS       XP_034196166             330 aa            linear   INV 03-SEP-2025
DEFINITION  goosecoid homeobox [Osmia lignaria lignaria].
ACCESSION   XP_034196166
VERSION     XP_034196166.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340275.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="goosecoid homeobox"
                     /calculated_mol_wt=37072
     Region          215..271
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..330
                     /gene="Gsc"
                     /coded_by="XM_034340275.2:7046..8038"
                     /db_xref="GeneID:117611867"
ORIGIN      
        1 mvesiyasds prsrspmtep ssplqdgpgi sgyshrylyn yspeslqlkq kssdeqnnva
       61 grrhntektf srspvsseet rfrardvsrp sssatmsspt saftienila prpignvtpt
      121 stsglgsiiq npetvgspsr taamhplqqq ihhlaftsad lfaaaypsly sggyvaamaa
      181 agvslhgqpa fyhaahpyqi npnvtavgpm tkrkrrhrti fteeqlerle atfdkthypd
      241 vilreqlalq vdlkeeriev wfknrrakwr kqkreeqerm rklqleqrqr sddnvstatv
      301 kvadhltlar qqqqqqqheq dtdssdleva
//
LOCUS       XP_034196167             280 aa            linear   INV 03-SEP-2025
DEFINITION  DNA-binding protein D-ETS-6 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196167
VERSION     XP_034196167.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340276.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196167.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..280
                     /product="DNA-binding protein D-ETS-6 isoform X1"
                     /calculated_mol_wt=31030
     Region          11..74
                     /region_name="SAM_PNT"
                     /note="Sterile alpha motif (SAM)/Pointed domain; cd08203"
                     /db_xref="CDD:188876"
     Region          121..206
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..280
                     /gene="LOC117611868"
                     /coded_by="XM_034340276.2:1645..2487"
                     /db_xref="GeneID:117611868"
ORIGIN      
        1 metepvmvps dpskwnsnhi aswiswcsra fsmksppdpg kfpstgkell klssdfwqgi
       61 pggkifakhl gylqfqvtgi qtpdllqeek iderfsllqr scsligstag asgsgavggg
      121 qvqlwqflle llsdssnssc iawegsngef kltdpdevar rwgerkskpn mnydklsral
      181 ryyydknimt kvhgkryayk fdfhglmmac qaqagvtlet ssssrstgtn chphhshhah
      241 hlyptgasns qhssvsahpp ppppppphyc wpyhrysppt
//
LOCUS       XP_076546746             215 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional regulator ERG homolog isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546746
VERSION     XP_076546746.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690631.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..215
                     /product="transcriptional regulator ERG homolog isoform
                     X2"
                     /calculated_mol_wt=23968
     Region          56..141
                     /region_name="ETS"
                     /note="erythroblast transformation specific domain;
                     smart00413"
                     /db_xref="CDD:197710"
     CDS             1..215
                     /gene="LOC117611868"
                     /coded_by="XM_076690631.1:823..1470"
                     /db_xref="GeneID:117611868"
ORIGIN      
        1 mwriqthplh lqqetrfhps aaailynerf sllqrscsli gstagasgsg avgggqvqlw
       61 qfllellsds snssciaweg sngefkltdp devarrwger kskpnmnydk lsralryyyd
      121 knimtkvhgk ryaykfdfhg lmmacqaqag vtletssssr stgtnchphh shhahhlypt
      181 gasnsqhssv sahppppppp pphycwpyhr ysppt
//
LOCUS       XP_034194885             183 aa            linear   INV 03-SEP-2025
DEFINITION  male-enhanced antigen 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194885
VERSION     XP_034194885.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194885.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..183
                     /product="male-enhanced antigen 1 isoform X1"
                     /calculated_mol_wt=20509
     Region          66..181
                     /region_name="MEA1"
                     /note="Male enhanced antigen 1 (MEA1); pfam06910"
                     /db_xref="CDD:429188"
     CDS             1..183
                     /gene="LOC117611095"
                     /coded_by="XM_034338994.2:19..570"
                     /db_xref="GeneID:117611095"
ORIGIN      
        1 mnsgihavlt cffrlqvkla vkiekrklct mspdptqepa datlnapnll letertndsd
       61 seddvgmagy vplsqiptds dpilyededd ewisnageps qtlppmlles qqdymsetie
      121 vwsspcnrsn idmdadkisq vksmmasftl pttaipewak tiseeqwkeq ligrikeiqn
      181 rek
//
LOCUS       XP_034194894             153 aa            linear   INV 03-SEP-2025
DEFINITION  male-enhanced antigen 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194894
VERSION     XP_034194894.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="male-enhanced antigen 1 isoform X2"
                     /calculated_mol_wt=17079
     Region          36..151
                     /region_name="MEA1"
                     /note="Male enhanced antigen 1 (MEA1); pfam06910"
                     /db_xref="CDD:429188"
     CDS             1..153
                     /gene="LOC117611095"
                     /coded_by="XM_034339003.2:191..652"
                     /db_xref="GeneID:117611095"
ORIGIN      
        1 mspdptqepa datlnapnll letertndsd seddvgmagy vplsqiptds dpilyededd
       61 ewisnageps qtlppmlles qqdymsetie vwsspcnrsn idmdadkisq vksmmasftl
      121 pttaipewak tiseeqwkeq ligrikeiqn rek
//
LOCUS       XP_034194904             153 aa            linear   INV 03-SEP-2025
DEFINITION  male-enhanced antigen 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194904
VERSION     XP_034194904.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339013.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="male-enhanced antigen 1 isoform X2"
                     /calculated_mol_wt=17079
     Region          36..151
                     /region_name="MEA1"
                     /note="Male enhanced antigen 1 (MEA1); pfam06910"
                     /db_xref="CDD:429188"
     CDS             1..153
                     /gene="LOC117611095"
                     /coded_by="XM_034339013.2:172..633"
                     /db_xref="GeneID:117611095"
ORIGIN      
        1 mspdptqepa datlnapnll letertndsd seddvgmagy vplsqiptds dpilyededd
       61 ewisnageps qtlppmlles qqdymsetie vwsspcnrsn idmdadkisq vksmmasftl
      121 pttaipewak tiseeqwkeq ligrikeiqn rek
//
LOCUS       XP_076546747             141 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S12 [Osmia lignaria lignaria].
ACCESSION   XP_076546747
VERSION     XP_076546747.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690632.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..141
                     /product="ribosomal protein S12"
                     /calculated_mol_wt=15087
     Region          23..118
                     /region_name="Ribosomal_L7Ae"
                     /note="Ribosomal protein L7Ae/L30e/S12e/Gadd45 family;
                     pfam01248"
                     /db_xref="CDD:426153"
     CDS             1..141
                     /gene="RpS12"
                     /coded_by="XM_076690632.1:119..544"
                     /db_xref="GeneID:117611870"
ORIGIN      
        1 msdvenddvp samaaggsmd vitalqevlk navihdgvvh glheaakald krqamlcila
       61 dncdepmykk lvqalcnehq iplvkvdnnk klgewaglck idsagkarkv vgcscvvikd
      121 fgedtpakdv vmeyvkqssv h
//
LOCUS       XP_034196168             141 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S12 [Osmia lignaria lignaria].
ACCESSION   XP_034196168
VERSION     XP_034196168.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340277.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..141
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..141
                     /product="ribosomal protein S12"
                     /calculated_mol_wt=15087
     Region          23..118
                     /region_name="Ribosomal_L7Ae"
                     /note="Ribosomal protein L7Ae/L30e/S12e/Gadd45 family;
                     pfam01248"
                     /db_xref="CDD:426153"
     CDS             1..141
                     /gene="RpS12"
                     /coded_by="XM_034340277.2:119..544"
                     /db_xref="GeneID:117611870"
ORIGIN      
        1 msdvenddvp samaaggsmd vitalqevlk navihdgvvh glheaakald krqamlcila
       61 dncdepmykk lvqalcnehq iplvkvdnnk klgewaglck idsagkarkv vgcscvvikd
      121 fgedtpakdv vmeyvkqssv h
//
LOCUS       XP_076546748             819 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger MYND-type containing 10 [Osmia lignaria lignaria].
ACCESSION   XP_076546748
VERSION     XP_076546748.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690633.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..819
                     /product="zinc finger MYND-type containing 10"
                     /calculated_mol_wt=95009
     Region          4..46
                     /region_name="RING_Ubox"
                     /note="RING finger (Really Interesting New Gene) domain
                     and U-box domain superfamily; cl17238"
                     /db_xref="CDD:473075"
     Site            order(5,8,23,25,28,31,42,45)
                     /site_type="other"
                     /note="cross-brace motif"
                     /db_xref="CDD:438111"
     Region          69..>312
                     /region_name="EnvC"
                     /note="Septal ring factor EnvC, activator of murein
                     hydrolases AmiA and AmiB [Cell cycle control, cell
                     division, chromosome partitioning]; COG4942"
                     /db_xref="CDD:443969"
     Region          770..806
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     CDS             1..819
                     /gene="Zmynd10"
                     /coded_by="XM_076690633.1:400..2859"
                     /db_xref="GeneID:117611864"
ORIGIN      
        1 mnvicsicsd plvqtddify trcghvfhlq clnawlersk scpqcrekvt qskihrlyft
       61 fanneivesq astlqdkidg lkfqillkek diqyytsknv tlekqnaglr qevykleaei
      121 nqknsaihal keqieyfkkr ssksdnlkke itqlkdkled lkplqallhg plsdvsrmig
      181 ndkdpdtlik fisimkkeli gsfnnckqlr mtvkrlreel slvnaklnsl lekqskqtdl
      241 eegptfshsq nktlqstdne leeiheihdk snnvseiqem endifkqqpv evktkkdksn
      301 keslgknvip vkrkkhktsi crnndedkne ykvqggktet dsihsdtdtk wnssndsptv
      361 skkyrmsdnn intsdtsnvs sssvskkkkk hstkikvsnt eddacsvidl twlefhkrli
      421 llnqqnvlei nalreenvke wfvsfkkipi liyeaiqidv wkhkvfpilv emnaepsntf
      481 mhftifyhen ivvsllesvl fhsesaetmd dssldlvdya vkyvsslldi qdieiyenlk
      541 dpnscleeil ekkkelefdi smrcisilry laeyadnlpi cvlsrllath dvpyllaqli
      601 enqpwkkena egesmiynsg wkkvksseeg kiskiegqvw fglrelllnp ksapyyeite
      661 hrltqliklq kylhetvldq isplidlkrw lsyltmsqcq srgpltvnve lipqikssim
      721 ekyqkkwkkl akyqskllyt kdaefiknaa kilsdaydld klgwidpkec flchedakkr
      781 cskckeawyc grqcqvkdwe shknicdkit ktvkdeqqd
//
LOCUS       XP_034195969            1177 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611792 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195969
VERSION     XP_034195969.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340078.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195969.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1177
                     /product="uncharacterized protein LOC117611792 isoform X1"
                     /calculated_mol_wt=128414
     Region          2..73
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,44,46,48..51,69..73)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          294..401
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1177
                     /gene="LOC117611792"
                     /coded_by="XM_034340078.2:148..3681"
                     /db_xref="GeneID:117611792"
ORIGIN      
        1 midltvktld sqnhafsled dqitvrgfke yiaesvavpa dsqrliycgr vlqdekklnd
       61 ydvngkvihl vqrappqpgq rgndggqnhg shgwqnaqrt hyrltraqmh gnamylgams
      121 vpaeiveghg lalpqfsntl sngrlivarh mvertnrlmd rlenpsrpvn laasennrpa
      181 lgelmqesel epeqfvfsni egrtnngail aeaaaaaaiv aalsaagann vtplragssd
      241 etaearptte aaeenqtdsq qpqqpqaeqq gstsntdgqs nrraspqlpr ppqlaellem
      301 lqdtqarlrp vldryckimr ddpslppggr rveenqayvd ganeclhyms hachalsdii
      361 vdmsqqpprn lrcrpiiiqh sailqpgipi qveahislhg rnannnngne esgdstnnas
      421 qpestesnae dssqqpepes atqqqaeqpq eqaqspfgtv fnlpnnvevl mevspesnmd
      481 aasgseqtqt gennnnnnnn nvggrvggta ggfswgsapa pdfirnlmqa vaghmvqsgm
      541 tnttittrnp vtvgqqnvtt mdsgntnagq stqarsnvgt hpttatqtrs tsrphvfhqq
      601 ahplgvgmsi gqafdfdpfl pcnshhvrrt sttssntvts tsqstqtsqt atsetqsqaq
      661 tattssatsn tsstnttast tsqtntndpf mflpqqilgg rsgqpqasis insnnhdlae
      721 slgnilqlfs grnihvgivg dsgnptgsdv tfadllehag iqmnllssqd sesegdllld
      781 lavlvvqnlt legiirlrtg qwepishlrr plqeflqysf pntstealqe qaaerllsql
      841 rplfqnlfas eeevnirngs ridicatvea llrrhikdil qflfnndidd arfgqeifni
      901 vnnlgrqlca vlrysirggq sglevvvsrf mtemlegtrp efhrwlpnif vaqlrtyssr
      961 vsqppeseil pllvykdvps qpstvpsapt hqpsqtqsev epmeteaiee rttfeaslpd
     1021 dgeevpetfp ghealpsdwv piiardgvrq rrqlqmqgmt nggvatfsda ylgtlptkrr
     1081 klieqqkprl lvsptpnhsa iaasmerlvr egvtragvee vegaavavav dpgvrrafgq
     1141 airdclnprr ygtpdfpdpi rfpnatkyfs dqdrqpk
//
LOCUS       XP_034195971            1176 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611792 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195971
VERSION     XP_034195971.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340080.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195971.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1176
                     /product="uncharacterized protein LOC117611792 isoform X2"
                     /calculated_mol_wt=128343
     Region          2..73
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,44,46,48..51,69..73)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          293..400
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1176
                     /gene="LOC117611792"
                     /coded_by="XM_034340080.2:148..3678"
                     /db_xref="GeneID:117611792"
ORIGIN      
        1 midltvktld sqnhafsled dqitvrgfke yiaesvavpa dsqrliycgr vlqdekklnd
       61 ydvngkvihl vqrappqpgq rgndggqnhg shgwqnaqrt hyrltraqmh gnamylgams
      121 vpaeiveghg lalpqfsntl sngrlivarh mvertnrlmd rlenpsrpvn laasennrpa
      181 lgelmqesel epeqfvfsni egrtnngail aeaaaaaaiv aalsaagann vtplrgssde
      241 taearpttea aeenqtdsqq pqqpqaeqqg stsntdgqsn rraspqlprp pqlaelleml
      301 qdtqarlrpv ldryckimrd dpslppggrr veenqayvdg aneclhymsh achalsdiiv
      361 dmsqqpprnl rcrpiiiqhs ailqpgipiq veahislhgr nannnngnee sgdstnnasq
      421 pestesnaed ssqqpepesa tqqqaeqpqe qaqspfgtvf nlpnnvevlm evspesnmda
      481 asgseqtqtg ennnnnnnnn vggrvggtag gfswgsapap dfirnlmqav aghmvqsgmt
      541 nttittrnpv tvgqqnvttm dsgntnagqs tqarsnvgth pttatqtrst srphvfhqqa
      601 hplgvgmsig qafdfdpflp cnshhvrrts ttssntvtst sqstqtsqta tsetqsqaqt
      661 attssatsnt sstnttastt sqtntndpfm flpqqilggr sgqpqasisi nsnnhdlaes
      721 lgnilqlfsg rnihvgivgd sgnptgsdvt fadllehagi qmnllssqds esegdllldl
      781 avlvvqnltl egiirlrtgq wepishlrrp lqeflqysfp ntstealqeq aaerllsqlr
      841 plfqnlfase eevnirngsr idicatveal lrrhikdilq flfnndidda rfgqeifniv
      901 nnlgrqlcav lrysirggqs glevvvsrfm temlegtrpe fhrwlpnifv aqlrtyssrv
      961 sqppeseilp llvykdvpsq pstvpsapth qpsqtqseve pmeteaieer ttfeaslpdd
     1021 geevpetfpg healpsdwvp iiardgvrqr rqlqmqgmtn ggvatfsday lgtlptkrrk
     1081 lieqqkprll vsptpnhsai aasmerlvre gvtragveev egaavavavd pgvrrafgqa
     1141 irdclnprry gtpdfpdpir fpnatkyfsd qdrqpk
//
LOCUS       XP_034195972            1175 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611792 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195972
VERSION     XP_034195972.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340081.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195972.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1175
                     /product="uncharacterized protein LOC117611792 isoform X3"
                     /calculated_mol_wt=128186
     Region          2..73
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,44,46,48..51,69..73)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          294..395
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1175
                     /gene="LOC117611792"
                     /coded_by="XM_034340081.2:148..3675"
                     /db_xref="GeneID:117611792"
ORIGIN      
        1 midltvktld sqnhafsled dqitvrgfke yiaesvavpa dsqrliycgr vlqdekklnd
       61 ydvngkvihl vqrappqpgq rgndggqnhg shgwqnaqrt hyrltraqmh gnamylgams
      121 vpaeiveghg lalpqfsntl sngrlivarh mvertnrlmd rlenpsrpvn laasennrpa
      181 lgelmqesel epeqfvfsni egrtnngail aeaaaaaaiv aalsaagann vtplragssd
      241 etaearptte aaeenqtdsq qpqqpqaeqq gstsntdgqs nrraspqlpr ppqlaellem
      301 lqdtqarlrp vldryckimr ddpslppggr rveenqayvd ganeclhyms hachalsdii
      361 vdmsqqpprn lrcrpiiiqh sailqpgipi qahislhgrn annnngnees gdstnnasqp
      421 estesnaeds sqqpepesat qqqaeqpqeq aqspfgtvfn lpnnvevlme vspesnmdaa
      481 sgseqtqtge nnnnnnnnnv ggrvggtagg fswgsapapd firnlmqava ghmvqsgmtn
      541 ttittrnpvt vgqqnvttmd sgntnagqst qarsnvgthp ttatqtrsts rphvfhqqah
      601 plgvgmsigq afdfdpflpc nshhvrrtst tssntvtsts qstqtsqtat setqsqaqta
      661 ttssatsnts stnttastts qtntndpfmf lpqqilggrs gqpqasisin snnhdlaesl
      721 gnilqlfsgr nihvgivgds gnptgsdvtf adllehagiq mnllssqdse segdllldla
      781 vlvvqnltle giirlrtgqw epishlrrpl qeflqysfpn tstealqeqa aerllsqlrp
      841 lfqnlfasee evnirngsri dicatveall rrhikdilqf lfnndiddar fgqeifnivn
      901 nlgrqlcavl rysirggqsg levvvsrfmt emlegtrpef hrwlpnifva qlrtyssrvs
      961 qppeseilpl lvykdvpsqp stvpsapthq psqtqsevep meteaieert tfeaslpddg
     1021 eevpetfpgh ealpsdwvpi iardgvrqrr qlqmqgmtng gvatfsdayl gtlptkrrkl
     1081 ieqqkprllv sptpnhsaia asmerlvreg vtragveeve gaavavavdp gvrrafgqai
     1141 rdclnprryg tpdfpdpirf pnatkyfsdq drqpk
//
LOCUS       XP_034195973            1174 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611792 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195973
VERSION     XP_034195973.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340082.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195973.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1174
                     /product="uncharacterized protein LOC117611792 isoform X4"
                     /calculated_mol_wt=128081
     Region          2..73
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,44,46,48..51,69..73)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          291..398
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1174
                     /gene="LOC117611792"
                     /coded_by="XM_034340082.2:148..3672"
                     /db_xref="GeneID:117611792"
ORIGIN      
        1 midltvktld sqnhafsled dqitvrgfke yiaesvavpa dsqrliycgr vlqdekklnd
       61 ydvngkvihl vqrappqpgq rgndggqnhg shgwqnaqrt hyrltraqmh gnamylgams
      121 vpaeiveghg lalpqfsntl sngrlivarh mvertnrlmd rlenpsrpvn laasennrpa
      181 lgelmqesel epeqfniegr tnngailaea aaaaaivaal saagannvtp lragssdeta
      241 earptteaae enqtdsqqpq qpqaeqqgst sntdgqsnrr aspqlprppq laellemlqd
      301 tqarlrpvld ryckimrddp slppggrrve enqayvdgan eclhymshac halsdiivdm
      361 sqqpprnlrc rpiiiqhsai lqpgipiqve ahislhgrna nnnngneesg dstnnasqpe
      421 stesnaedss qqpepesatq qqaeqpqeqa qspfgtvfnl pnnvevlmev spesnmdaas
      481 gseqtqtgen nnnnnnnnvg grvggtaggf swgsapapdf irnlmqavag hmvqsgmtnt
      541 tittrnpvtv gqqnvttmds gntnagqstq arsnvgthpt tatqtrstsr phvfhqqahp
      601 lgvgmsigqa fdfdpflpcn shhvrrtstt ssntvtstsq stqtsqtats etqsqaqtat
      661 tssatsntss tnttasttsq tntndpfmfl pqqilggrsg qpqasisins nnhdlaeslg
      721 nilqlfsgrn ihvgivgdsg nptgsdvtfa dllehagiqm nllssqdses egdllldlav
      781 lvvqnltleg iirlrtgqwe pishlrrplq eflqysfpnt stealqeqaa erllsqlrpl
      841 fqnlfaseee vnirngsrid icatveallr rhikdilqfl fnndiddarf gqeifnivnn
      901 lgrqlcavlr ysirggqsgl evvvsrfmte mlegtrpefh rwlpnifvaq lrtyssrvsq
      961 ppeseilpll vykdvpsqps tvpsapthqp sqtqsevepm eteaieertt feaslpddge
     1021 evpetfpghe alpsdwvpii ardgvrqrrq lqmqgmtngg vatfsdaylg tlptkrrkli
     1081 eqqkprllvs ptpnhsaiaa smerlvregv tragveeveg aavavavdpg vrrafgqair
     1141 dclnprrygt pdfpdpirfp natkyfsdqd rqpk
//
LOCUS       XP_034195974            1173 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611792 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195974
VERSION     XP_034195974.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340083.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195974.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1173
                     /product="uncharacterized protein LOC117611792 isoform X5"
                     /calculated_mol_wt=128010
     Region          2..73
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,44,46,48..51,69..73)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          290..397
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1173
                     /gene="LOC117611792"
                     /coded_by="XM_034340083.2:148..3669"
                     /db_xref="GeneID:117611792"
ORIGIN      
        1 midltvktld sqnhafsled dqitvrgfke yiaesvavpa dsqrliycgr vlqdekklnd
       61 ydvngkvihl vqrappqpgq rgndggqnhg shgwqnaqrt hyrltraqmh gnamylgams
      121 vpaeiveghg lalpqfsntl sngrlivarh mvertnrlmd rlenpsrpvn laasennrpa
      181 lgelmqesel epeqfniegr tnngailaea aaaaaivaal saagannvtp lrgssdetae
      241 arptteaaee nqtdsqqpqq pqaeqqgsts ntdgqsnrra spqlprppql aellemlqdt
      301 qarlrpvldr yckimrddps lppggrrvee nqayvdgane clhymshach alsdiivdms
      361 qqpprnlrcr piiiqhsail qpgipiqvea hislhgrnan nnngneesgd stnnasqpes
      421 tesnaedssq qpepesatqq qaeqpqeqaq spfgtvfnlp nnvevlmevs pesnmdaasg
      481 seqtqtgenn nnnnnnnvgg rvggtaggfs wgsapapdfi rnlmqavagh mvqsgmtntt
      541 ittrnpvtvg qqnvttmdsg ntnagqstqa rsnvgthptt atqtrstsrp hvfhqqahpl
      601 gvgmsigqaf dfdpflpcns hhvrrtstts sntvtstsqs tqtsqtatse tqsqaqtatt
      661 ssatsntsst nttasttsqt ntndpfmflp qqilggrsgq pqasisinsn nhdlaeslgn
      721 ilqlfsgrni hvgivgdsgn ptgsdvtfad llehagiqmn llssqdsese gdllldlavl
      781 vvqnltlegi irlrtgqwep ishlrrplqe flqysfpnts tealqeqaae rllsqlrplf
      841 qnlfaseeev nirngsridi catveallrr hikdilqflf nndiddarfg qeifnivnnl
      901 grqlcavlry sirggqsgle vvvsrfmtem legtrpefhr wlpnifvaql rtyssrvsqp
      961 peseilpllv ykdvpsqpst vpsapthqps qtqsevepme teaieerttf easlpddgee
     1021 vpetfpghea lpsdwvpiia rdgvrqrrql qmqgmtnggv atfsdaylgt lptkrrklie
     1081 qqkprllvsp tpnhsaiaas merlvregvt ragveevega avavavdpgv rrafgqaird
     1141 clnprrygtp dfpdpirfpn atkyfsdqdr qpk
//
LOCUS       XP_034195975            1165 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611792 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195975
VERSION     XP_034195975.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340084.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195975.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1165
                     /product="uncharacterized protein LOC117611792 isoform X6"
                     /calculated_mol_wt=127102
     Region          2..73
                     /region_name="Ubl_BAG6"
                     /note="ubiquitin-like (Ubl) domain found in
                     BCL2-associated athanogene 6 (BAG6) and similar proteins;
                     cd01809"
                     /db_xref="CDD:340507"
     Site            order(7..10,44,46,48..51,69..73)
                     /site_type="other"
                     /note="ZF interaction site [polypeptide binding]"
                     /db_xref="CDD:340507"
     Region          294..401
                     /region_name="BAG6"
                     /note="BCL2-associated athanogene 6; pfam12057"
                     /db_xref="CDD:463449"
     CDS             1..1165
                     /gene="LOC117611792"
                     /coded_by="XM_034340084.2:148..3645"
                     /db_xref="GeneID:117611792"
ORIGIN      
        1 midltvktld sqnhafsled dqitvrgfke yiaesvavpa dsqrliycgr vlqdekklnd
       61 ydvngkvihl vqrappqpgq rgndggqnhg shgwqnaqrt hyrltraqmh gnamylgams
      121 vpaeiveghg lalpqfsntl sngrlivarh mvertnrlmd rlenpsrpvn laasennrpa
      181 lgelmqesel epeqfvfsni egrtnngail aeaaaaaaiv aalsaagann vtplragssd
      241 etaearptte aaeenqtdsq qpqqpqaeqq gstsntdgqs nrraspqlpr ppqlaellem
      301 lqdtqarlrp vldryckimr ddpslppggr rveenqayvd ganeclhyms hachalsdii
      361 vdmsqqpprn lrcrpiiiqh sailqpgipi qveahislhg rnannnngne esgdstnnas
      421 qpestesnae dssqqpepes atqqqaeqpq eqaqspfgtv fnlpnnvevl mevspesnmd
      481 aasgseqtqt ggrvggtagg fswgsapapd firnlmqava ghmvqsgmtn ttittrnpvt
      541 vgqqnvttmd sgntnagqst qarsnvgthp ttatqtrsts rphvfhqqah plgvgmsigq
      601 afdfdpflpc nshhvrrtst tssntvtsts qstqtsqtat setqsqaqta ttssatsnts
      661 stnttastts qtntndpfmf lpqqilggrs gqpqasisin snnhdlaesl gnilqlfsgr
      721 nihvgivgds gnptgsdvtf adllehagiq mnllssqdse segdllldla vlvvqnltle
      781 giirlrtgqw epishlrrpl qeflqysfpn tstealqeqa aerllsqlrp lfqnlfasee
      841 evnirngsri dicatveall rrhikdilqf lfnndiddar fgqeifnivn nlgrqlcavl
      901 rysirggqsg levvvsrfmt emlegtrpef hrwlpnifva qlrtyssrvs qppeseilpl
      961 lvykdvpsqp stvpsapthq psqtqsevep meteaieert tfeaslpddg eevpetfpgh
     1021 ealpsdwvpi iardgvrqrr qlqmqgmtng gvatfsdayl gtlptkrrkl ieqqkprllv
     1081 sptpnhsaia asmerlvreg vtragveeve gaavavavdp gvrrafgqai rdclnprryg
     1141 tpdfpdpirf pnatkyfsdq drqpk
//
LOCUS       XP_034193102             798 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho [Osmia lignaria lignaria].
ACCESSION   XP_034193102
VERSION     XP_034193102.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337211.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..798
                     /product="protein groucho"
                     /calculated_mol_wt=85320
     Region          31..155
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <509..796
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          513..554
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          562..600
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          605..641
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          648..683
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          688..721
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          729..763
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          770..794
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..798
                     /gene="LOC117610156"
                     /coded_by="XM_034337211.2:387..2783"
                     /db_xref="GeneID:117610156"
ORIGIN      
        1 mypnaginaa aavavaaaar hpgppqpgqq ikftvgescd rikeefnflq nqchalklec
       61 eklatekiei qrhyvmyyem syglnvemhk qaeiskrlna iigqilpyls qeqqqqvata
      121 vdrakqvtmt elnsiigqqr pdisrllqqm haqqlppgaa igahpglpgl pglgpaaglp
      181 vptsasaall slgltpgaat apgttaahls mlskpelhrg qpddlksngg lssteerhrs
      241 sispgdkysr prtpqdahhs hhsqnhhdhp mhikkmkkeq dkdighsdge ksdqdlvvdd
      301 asegptspvv ngttspreng ldkvvppmga gvqtkkdvpp hsprsgtssn astpsakkme
      361 erekpttpis kpltptsasg iggagmkisg sakllqgppp pgvpsaypph yapgppphhl
      421 apagqhphvd mmgyngyvap rappslqqly dphvamrapm gsvgvpggkp aysfhvsseg
      481 qmqpvpfppd aligqgiprh arqintlshg evvcavtisn ptkyvytggk gcvkvwdigq
      541 ggtgstksvs qldclqpdny irsvkllpdg rtlivggeas rlsiwdlasp tprikaelis
      601 sapacyalai spdskvcfsc csdgniavwd lqnqtlvrqf qghtdgasci disadgsklw
      661 tggldntvrs wdlregrqlq qhdftsqifs lgycptgewl avgmensnve vlhaskpdky
      721 qlhlhescvl slrfascgkw fvstgkdnll nawrtpygas ifqskesssv lscdisaddr
      781 yivtgsgdkk atvyevmy
//
LOCUS       XP_034195981             633 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat-containing larval translucida [Osmia lignaria
            lignaria].
ACCESSION   XP_034195981
VERSION     XP_034195981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="leucine-rich repeat-containing larval
                     translucida"
                     /calculated_mol_wt=69783
     Region          97..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          120..143
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <126..>362
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          144..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          168..191
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          192..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          215..236
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          237..260
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          261..283
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          284..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          307..325
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          367..426
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          426..486
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          427..450
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          451..475
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          475..534
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          476..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..523
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          524..544
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..633
                     /gene="ltl"
                     /coded_by="XM_034340090.2:4186..6087"
                     /db_xref="GeneID:117611796"
ORIGIN      
        1 msgllytllg laastslhca vrreispctc rqeefstsvi npaqtavava tavvntgnna
       61 ghhghgerie vvcermdsfe qvagalrgkf taeqqitlrv shsnlrdisr hdfkelrmsi
      121 trlelnhdhl gfvdgevfag lgrtqylsla dnevpsiprh ilshlsllrt ldlsrnrisr
      181 idsddfkynp tlqhlsmagn aisemvpgsl pplvkhlhvg rnhlqslnrt lrdlnqlewl
      241 linaneltsl dgelpssghn lkmlyavdnk lthlpaefry lhrletlflq hnkirsldgt
      301 lqkarrlkfl elsyndlqel teadfveaem ledlelghns lqslggsngg ngdgsggnsv
      361 lyplrslkcl nlthnelref sfaslrglre lrlldlsnnr igqlhrgrtp senlveeege
      421 etaggniqdm rlqhnelrsl dgslflgmke lqrlnlshna lgptigprdl rgldglkvld
      481 lshnelttle dtsetwlpsl eelnashnrl vtlserdfrg fpvlcwadvs anrirtlmpe
      541 lvantrctvh gvpdvlriyl qdnpvlcdpg ladltvalev nhakvygvvn ncpttpaapt
      601 eqtsplpplp ptlllaaaaa asggnvsfaa tle
//
LOCUS       XP_034195982             633 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat-containing larval translucida [Osmia lignaria
            lignaria].
ACCESSION   XP_034195982
VERSION     XP_034195982.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340091.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="leucine-rich repeat-containing larval
                     translucida"
                     /calculated_mol_wt=69783
     Region          97..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          120..143
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <126..>362
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          144..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          168..191
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          192..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          215..236
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          237..260
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          261..283
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          284..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          307..325
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          367..426
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          426..486
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          427..450
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          451..475
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          475..534
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          476..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..523
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          524..544
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..633
                     /gene="ltl"
                     /coded_by="XM_034340091.2:4124..6025"
                     /db_xref="GeneID:117611796"
ORIGIN      
        1 msgllytllg laastslhca vrreispctc rqeefstsvi npaqtavava tavvntgnna
       61 ghhghgerie vvcermdsfe qvagalrgkf taeqqitlrv shsnlrdisr hdfkelrmsi
      121 trlelnhdhl gfvdgevfag lgrtqylsla dnevpsiprh ilshlsllrt ldlsrnrisr
      181 idsddfkynp tlqhlsmagn aisemvpgsl pplvkhlhvg rnhlqslnrt lrdlnqlewl
      241 linaneltsl dgelpssghn lkmlyavdnk lthlpaefry lhrletlflq hnkirsldgt
      301 lqkarrlkfl elsyndlqel teadfveaem ledlelghns lqslggsngg ngdgsggnsv
      361 lyplrslkcl nlthnelref sfaslrglre lrlldlsnnr igqlhrgrtp senlveeege
      421 etaggniqdm rlqhnelrsl dgslflgmke lqrlnlshna lgptigprdl rgldglkvld
      481 lshnelttle dtsetwlpsl eelnashnrl vtlserdfrg fpvlcwadvs anrirtlmpe
      541 lvantrctvh gvpdvlriyl qdnpvlcdpg ladltvalev nhakvygvvn ncpttpaapt
      601 eqtsplpplp ptlllaaaaa asggnvsfaa tle
//
LOCUS       XP_076546749             633 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat-containing larval translucida [Osmia lignaria
            lignaria].
ACCESSION   XP_076546749
VERSION     XP_076546749.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690634.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="leucine-rich repeat-containing larval
                     translucida"
                     /calculated_mol_wt=69783
     Region          97..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          120..143
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <126..>362
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          144..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          168..191
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          192..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          215..236
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          237..260
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          261..283
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          284..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          307..325
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          367..426
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          426..486
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          427..450
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          451..475
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          475..534
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          476..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..523
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          524..544
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..633
                     /gene="ltl"
                     /coded_by="XM_076690634.1:3040..4941"
                     /db_xref="GeneID:117611796"
ORIGIN      
        1 msgllytllg laastslhca vrreispctc rqeefstsvi npaqtavava tavvntgnna
       61 ghhghgerie vvcermdsfe qvagalrgkf taeqqitlrv shsnlrdisr hdfkelrmsi
      121 trlelnhdhl gfvdgevfag lgrtqylsla dnevpsiprh ilshlsllrt ldlsrnrisr
      181 idsddfkynp tlqhlsmagn aisemvpgsl pplvkhlhvg rnhlqslnrt lrdlnqlewl
      241 linaneltsl dgelpssghn lkmlyavdnk lthlpaefry lhrletlflq hnkirsldgt
      301 lqkarrlkfl elsyndlqel teadfveaem ledlelghns lqslggsngg ngdgsggnsv
      361 lyplrslkcl nlthnelref sfaslrglre lrlldlsnnr igqlhrgrtp senlveeege
      421 etaggniqdm rlqhnelrsl dgslflgmke lqrlnlshna lgptigprdl rgldglkvld
      481 lshnelttle dtsetwlpsl eelnashnrl vtlserdfrg fpvlcwadvs anrirtlmpe
      541 lvantrctvh gvpdvlriyl qdnpvlcdpg ladltvalev nhakvygvvn ncpttpaapt
      601 eqtsplpplp ptlllaaaaa asggnvsfaa tle
//
LOCUS       XP_034195984             633 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat-containing larval translucida [Osmia lignaria
            lignaria].
ACCESSION   XP_034195984
VERSION     XP_034195984.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340093.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..633
                     /product="leucine-rich repeat-containing larval
                     translucida"
                     /calculated_mol_wt=69783
     Region          97..119
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          120..143
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          <126..>362
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          144..167
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          168..191
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          192..212
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          215..236
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          237..260
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          261..283
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          284..306
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          307..325
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          367..426
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          426..486
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          427..450
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          451..475
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          475..534
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          476..499
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          500..523
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          524..544
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..633
                     /gene="ltl"
                     /coded_by="XM_034340093.2:2739..4640"
                     /db_xref="GeneID:117611796"
ORIGIN      
        1 msgllytllg laastslhca vrreispctc rqeefstsvi npaqtavava tavvntgnna
       61 ghhghgerie vvcermdsfe qvagalrgkf taeqqitlrv shsnlrdisr hdfkelrmsi
      121 trlelnhdhl gfvdgevfag lgrtqylsla dnevpsiprh ilshlsllrt ldlsrnrisr
      181 idsddfkynp tlqhlsmagn aisemvpgsl pplvkhlhvg rnhlqslnrt lrdlnqlewl
      241 linaneltsl dgelpssghn lkmlyavdnk lthlpaefry lhrletlflq hnkirsldgt
      301 lqkarrlkfl elsyndlqel teadfveaem ledlelghns lqslggsngg ngdgsggnsv
      361 lyplrslkcl nlthnelref sfaslrglre lrlldlsnnr igqlhrgrtp senlveeege
      421 etaggniqdm rlqhnelrsl dgslflgmke lqrlnlshna lgptigprdl rgldglkvld
      481 lshnelttle dtsetwlpsl eelnashnrl vtlserdfrg fpvlcwadvs anrirtlmpe
      541 lvantrctvh gvpdvlriyl qdnpvlcdpg ladltvalev nhakvygvvn ncpttpaapt
      601 eqtsplpplp ptlllaaaaa asggnvsfaa tle
//
LOCUS       XP_076546750             240 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611797 [Osmia lignaria lignaria].
ACCESSION   XP_076546750
VERSION     XP_076546750.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690635.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="uncharacterized protein LOC117611797"
                     /calculated_mol_wt=28355
     Region          57..>128
                     /region_name="CwlO1"
                     /note="Uncharacterized N-terminal coiled-coil domain of
                     peptidoglycan hydrolase CwlO [Function unknown]; COG3883"
                     /db_xref="CDD:443091"
     CDS             1..240
                     /gene="LOC117611797"
                     /coded_by="XM_076690635.1:95..817"
                     /db_xref="GeneID:117611797"
ORIGIN      
        1 meefscnieh vlqnrdfsil qkltsyysnf cnkiatnvkd vtahdieytk rrtksiskis
       61 elqqeienlk seidttklqq mivdkkisna itqqeklkad vddaklkrdn lsvemvdlqq
      121 estkrkeekl lawdaikrac kiykeyldis iqfiendgye rikvsffvnd dstrdkyyvy
      181 lnhsdnqwkv eqiqpilkme elnefkgiid fskqseisnv itfvcklrhi fvkhylnteq
//
LOCUS       XP_034196000            1709 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase I subunit RpI1 [Osmia lignaria lignaria].
ACCESSION   XP_034196000
VERSION     XP_034196000.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340109.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196000.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1709
                     /product="RNA polymerase I subunit RpI1"
                     /calculated_mol_wt=194426
     Region          22..1002
                     /region_name="RNAP_I_RPA1_N"
                     /note="Largest subunit (RPA1) of eukaryotic RNA polymerase
                     I (RNAP I), N-terminal domain; cd01435"
                     /db_xref="CDD:259844"
     Site            order(71,74,81,84)
                     /site_type="other"
                     /note="Zn-binding [ion binding]"
                     /db_xref="CDD:259844"
     Site            order(419,424,431,437,548..550,584,586,588,984..985)
                     /site_type="active"
                     /note="putative active site region [active]"
                     /db_xref="CDD:259844"
     Region          1158..>1222
                     /region_name="PRK14898"
                     /note="DNA-directed RNA polymerase subunit A'';
                     Provisional"
                     /db_xref="CDD:237854"
     Region          1193..1694
                     /region_name="RNAP_I_Rpa1_C"
                     /note="Largest subunit (Rpa1) of Eukaryotic RNA polymerase
                     I (RNAP I), C-terminal domain; cd02735"
                     /db_xref="CDD:132722"
     Site            order(1195..1196,1200,1203,1688,1691..1694)
                     /site_type="other"
                     /note="Rpb1 (Rpa1) - Rpb6 interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:132722"
     Site            order(1202..1249,1582..1639,1641..1645)
                     /site_type="active"
                     /note="cleft [active]"
                     /db_xref="CDD:132722"
     Site            order(1204,1207,1660,1670..1672,1678..1679,1681,1683,1688)
                     /site_type="other"
                     /note="Rpb1 (Rpa1) - Rpb2 (Rpa2) interaction site
                     [polypeptide binding]"
                     /db_xref="CDD:132722"
     Site            order(1249,1637,1653..1654,1657)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:132722"
     Site            order(1575..1576,1578,1588..1594,1596,1616,1627..1628,
                     1630)
                     /site_type="other"
                     /note="Rpb1 (Rpa1) - Rpb5 interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:132722"
     Site            1645..1686
                     /site_type="active"
                     /note="clamp [active]"
                     /db_xref="CDD:132722"
     CDS             1..1709
                     /gene="RpI1"
                     /coded_by="XM_034340109.2:193..5322"
                     /db_xref="GeneID:117611803"
ORIGIN      
        1 mvqykhrasr ltpkhldpkc lmfsmftand irnlsvtkir tplsfnilgh plkgglydpa
       61 lgpllessdp cgtcgcnvfk cpghfghiel pmpvvnplfh kglymliklt clkcftvqip
      121 ayvklllstk lkllqqgyls diegleqevm fivsdahtpy dvdlesvqnv idnyvenlyn
      181 rsrfnqsvir ydenqlstkn inmqwhtyve nvlkqyttpk icvncqetip rittlknkim
      241 ttkmsnienk eiqshvvihk letimilpdq skeylrklwq negdflkvii pcfgmvdiey
      301 ptdifffevi pvlppivrpv nfvngqmieh pqsqvyksii qdclvlrnii qtiqdgdtsq
      361 lpeegklvfe qikgntaiek lhnawqslqv nvdhlmdrdm nktadsancq glkqviekke
      421 gvirmhmmgk rvnfaarsvi tpdpnlnide igipetfalr ltyptpvtpw nvvhlrqlvs
      481 ngpnvhpgav mvenedgsvq risadniiqr eaiakrlltt sdkanqffkg ikiihrhlqn
      541 gdilllnrqp tlhkpsimah karilrgekt lrfhyancka ynadfdgdem nvhfpqnela
      601 rsegynianv snqylvpkdg tplsgliqdh mvsgvrltvr grffsredyq qlvyaalsti
      661 qdniillpps iikpkplwsg kqlvstiivn vippnlarin ltagakinak awqvakprrw
      721 kcgyefsdpk tmseaeviir ngellsgvld kthygatpfg lvhcvfelyg gtcsikllsa
      781 fgklfqaflq rsgftlgied illvkkadik rkeiiskcrt igeetqksll elpddtpmdv
      841 vtakmeesyw snpkfraqvd rkykialdvy tndinktclp agllkkfpdn nlqlmvqsga
      901 kgstvntmqi scllgqiele gkrpplmisg kslpsfpayd pspraggfid grfmtgikpq
      961 efffhcmagr eglidtavkt srsgylqrcl ikhlegliin ydstvrdsdg sliqiyyged
     1021 gldvpgsrfl rkeqidflmd nkdaiidekl mkylkevsnt drilklvkki kkwkskngsa
     1081 lekrrvspfa rfcvenanii kpskfkrink ssgrskgals lmrkwirtte eekqtinsrc
     1141 ikcpdpvmse yrqdidfgvi serleelidd ymtsksknav vrkkdlrdll smkvmkivcp
     1201 pgepvgllaa qsigepstqm tlntfhfagr gemnvtlgip rlreilmmas ksiktpsmei
     1261 pfkndlknlt kssnrlklkl tkcilsdvld nisitrrmeq fpnrrlvctm tinylphksy
     1321 kgefsvqpqd ilkkteeiff eemfkemfke ikkvvkvtgl lhveeekkst ydddalldqa
     1381 epdqgtestf ksranlgemh essdeeeaae dadatlarti srhkenqeye dpeeeeemed
     1441 vnerdddddv enenekikrt eqledydddd vdgrtevqys tqrrndittm ytqaleydyd
     1501 edkctwcklt fwlplkmikl dlptiirnva nkvilwevpc ikraftfqnn aretilktdg
     1561 iniiemfkyd rllnlnklys ndiygivqty gieaanriiv keirdvfkmy gitvdsrhls
     1621 liadymtfdg tfqplsrkgm edsasplqqi sfessltflk natlqgkrdn lvspssrlml
     1681 gqpckagtga csllvkiqtq peivlsgga
//
LOCUS       XP_076546751            1709 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase I subunit RpI1 [Osmia lignaria lignaria].
ACCESSION   XP_076546751
VERSION     XP_076546751.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690636.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1709
                     /product="RNA polymerase I subunit RpI1"
                     /calculated_mol_wt=194426
     Region          22..1002
                     /region_name="RNAP_I_RPA1_N"
                     /note="Largest subunit (RPA1) of eukaryotic RNA polymerase
                     I (RNAP I), N-terminal domain; cd01435"
                     /db_xref="CDD:259844"
     Site            order(71,74,81,84)
                     /site_type="other"
                     /note="Zn-binding [ion binding]"
                     /db_xref="CDD:259844"
     Site            order(419,424,431,437,548..550,584,586,588,984..985)
                     /site_type="active"
                     /note="putative active site region [active]"
                     /db_xref="CDD:259844"
     Region          1158..>1222
                     /region_name="PRK14898"
                     /note="DNA-directed RNA polymerase subunit A'';
                     Provisional"
                     /db_xref="CDD:237854"
     Region          1193..1694
                     /region_name="RNAP_I_Rpa1_C"
                     /note="Largest subunit (Rpa1) of Eukaryotic RNA polymerase
                     I (RNAP I), C-terminal domain; cd02735"
                     /db_xref="CDD:132722"
     Site            order(1195..1196,1200,1203,1688,1691..1694)
                     /site_type="other"
                     /note="Rpb1 (Rpa1) - Rpb6 interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:132722"
     Site            order(1202..1249,1582..1639,1641..1645)
                     /site_type="active"
                     /note="cleft [active]"
                     /db_xref="CDD:132722"
     Site            order(1204,1207,1660,1670..1672,1678..1679,1681,1683,1688)
                     /site_type="other"
                     /note="Rpb1 (Rpa1) - Rpb2 (Rpa2) interaction site
                     [polypeptide binding]"
                     /db_xref="CDD:132722"
     Site            order(1249,1637,1653..1654,1657)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:132722"
     Site            order(1575..1576,1578,1588..1594,1596,1616,1627..1628,
                     1630)
                     /site_type="other"
                     /note="Rpb1 (Rpa1) - Rpb5 interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:132722"
     Site            1645..1686
                     /site_type="active"
                     /note="clamp [active]"
                     /db_xref="CDD:132722"
     CDS             1..1709
                     /gene="RpI1"
                     /coded_by="XM_076690636.1:283..5412"
                     /db_xref="GeneID:117611803"
ORIGIN      
        1 mvqykhrasr ltpkhldpkc lmfsmftand irnlsvtkir tplsfnilgh plkgglydpa
       61 lgpllessdp cgtcgcnvfk cpghfghiel pmpvvnplfh kglymliklt clkcftvqip
      121 ayvklllstk lkllqqgyls diegleqevm fivsdahtpy dvdlesvqnv idnyvenlyn
      181 rsrfnqsvir ydenqlstkn inmqwhtyve nvlkqyttpk icvncqetip rittlknkim
      241 ttkmsnienk eiqshvvihk letimilpdq skeylrklwq negdflkvii pcfgmvdiey
      301 ptdifffevi pvlppivrpv nfvngqmieh pqsqvyksii qdclvlrnii qtiqdgdtsq
      361 lpeegklvfe qikgntaiek lhnawqslqv nvdhlmdrdm nktadsancq glkqviekke
      421 gvirmhmmgk rvnfaarsvi tpdpnlnide igipetfalr ltyptpvtpw nvvhlrqlvs
      481 ngpnvhpgav mvenedgsvq risadniiqr eaiakrlltt sdkanqffkg ikiihrhlqn
      541 gdilllnrqp tlhkpsimah karilrgekt lrfhyancka ynadfdgdem nvhfpqnela
      601 rsegynianv snqylvpkdg tplsgliqdh mvsgvrltvr grffsredyq qlvyaalsti
      661 qdniillpps iikpkplwsg kqlvstiivn vippnlarin ltagakinak awqvakprrw
      721 kcgyefsdpk tmseaeviir ngellsgvld kthygatpfg lvhcvfelyg gtcsikllsa
      781 fgklfqaflq rsgftlgied illvkkadik rkeiiskcrt igeetqksll elpddtpmdv
      841 vtakmeesyw snpkfraqvd rkykialdvy tndinktclp agllkkfpdn nlqlmvqsga
      901 kgstvntmqi scllgqiele gkrpplmisg kslpsfpayd pspraggfid grfmtgikpq
      961 efffhcmagr eglidtavkt srsgylqrcl ikhlegliin ydstvrdsdg sliqiyyged
     1021 gldvpgsrfl rkeqidflmd nkdaiidekl mkylkevsnt drilklvkki kkwkskngsa
     1081 lekrrvspfa rfcvenanii kpskfkrink ssgrskgals lmrkwirtte eekqtinsrc
     1141 ikcpdpvmse yrqdidfgvi serleelidd ymtsksknav vrkkdlrdll smkvmkivcp
     1201 pgepvgllaa qsigepstqm tlntfhfagr gemnvtlgip rlreilmmas ksiktpsmei
     1261 pfkndlknlt kssnrlklkl tkcilsdvld nisitrrmeq fpnrrlvctm tinylphksy
     1321 kgefsvqpqd ilkkteeiff eemfkemfke ikkvvkvtgl lhveeekkst ydddalldqa
     1381 epdqgtestf ksranlgemh essdeeeaae dadatlarti srhkenqeye dpeeeeemed
     1441 vnerdddddv enenekikrt eqledydddd vdgrtevqys tqrrndittm ytqaleydyd
     1501 edkctwcklt fwlplkmikl dlptiirnva nkvilwevpc ikraftfqnn aretilktdg
     1561 iniiemfkyd rllnlnklys ndiygivqty gieaanriiv keirdvfkmy gitvdsrhls
     1621 liadymtfdg tfqplsrkgm edsasplqqi sfessltflk natlqgkrdn lvspssrlml
     1681 gqpckagtga csllvkiqtq peivlsgga
//
LOCUS       XP_076546752            1350 aa            linear   INV 03-SEP-2025
DEFINITION  protein expanded isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546752
VERSION     XP_076546752.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690637.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1350
                     /product="protein expanded isoform X1"
                     /calculated_mol_wt=146168
     Region          20..119
                     /region_name="FERM_F1_PTPN13_like"
                     /note="FERM (Four.1 protein, Ezrin, Radixin, Moesin)
                     domain, F1 sub-domain, found in tyrosine-protein
                     phosphatase non-receptor type 13 (PTPN13) and similar
                     proteins; cd17101"
                     /db_xref="CDD:340621"
     Region          23..239
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          245..346
                     /region_name="FERM_C_FRMD1_FRMD6"
                     /note="FERM domain C-lobe of FERM domain containing 1 and
                     6 proteins; cd13185"
                     /db_xref="CDD:270006"
     Site            order(258,276,278,284)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270006"
     Site            order(288,293..296,326,330,333..334)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     Site            326..337
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     CDS             1..1350
                     /gene="LOC117611804"
                     /coded_by="XM_076690637.1:527..4579"
                     /db_xref="GeneID:117611804"
ORIGIN      
        1 mrgsgvtaar sclqplvsas rylavqalpg dplyfvveak srvkevyaqt cmllgqqgmr
       61 dcelfglail sdgeylfvdp enklskyapk nwrsshtygl dssgrpafvl yfrvryyidt
      121 plllsdettr hhyylqlrdn vvrhgggves lhphhplhep sivtplltla glalqadlgd
      181 yseerhrpha gavgyckptd ylpphmcles nvlgvlaaqh rdnrglsree aelqyireav
      241 lleaplnahl yrlrrsknea gpgrillaic argvrvyaee etprvfawnn igklcfdrkk
      301 feiravdqpd kltlysgcdd ksklllglcr dthqfsmaia prvneakrre eeerkvlrdc
      361 ymyparckls ltargargdq risvisstss nttsgivsdr vhsedepdta pasteclprl
      421 tenssshpgg gggggggclv ngsnadiedr smpsspastv devdgtdntp ttaalrlass
      481 aatgaegsqc ssscstvvvv napaggpprm rrtsatssle lgyshtaqns avssetasfp
      541 gaiydrckka gkyagtdias etsgvytlrs semqderigd lyspsgdane ssaasdmcal
      601 ssvqsttdea svtsgfytih sglrsetsdv ytedvgtddq epiysvckgd ddvandivvs
      661 amsavsldqq ledsrsrsns ilsagsfrgd gsdpssvgpl lsadelsdli vgrypprkti
      721 snsmdsdcdy vtmppppppp rtdsdrqlpp pppypvsmdy atinssraki pdierepppp
      781 ppynatrgef vplpprrtdp ppppppytsp reriqipppt pprndavqpr epppppppyp
      841 evsdvtrqea isklyptsst eeivsrvttv tsskfqsnlt nnksnngnit plnskttssi
      901 aanhtsnddv asiapkpppr iqpptypgdk mkptpvhapv aalvvgpnny ldvhasrggp
      961 vllpyltppp ppppppppmi hprqpppppp slatvytsqv arsqieqykq qlysdvdyvm
     1021 fplkdpavsk qeyidakqgs llaamaaypp ppypsfnnks lvmyrstpyl pgssfsspsk
     1081 yasnqnlsss dtssnnylph snlsshyaas tsslysgatc ssagsqslrr eppapthpht
     1141 lhafsrtrsd enilkcfdsp ssiklqslpq lkhrrlpppp ppppyeiqnl eknqplpsas
     1201 ssssspnkts ktmgteerer eegkengmld irtlreksrn ldlplisalc ndryllkqtk
     1261 afvmpkhppt ettssstsak ngtrtsnigt ssrnkypvsg lsttqiakpp rksststhkh
     1321 paevkgnayk vsnstavssv kkdptrashs
//
LOCUS       XP_076546753            1332 aa            linear   INV 03-SEP-2025
DEFINITION  protein expanded isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546753
VERSION     XP_076546753.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690638.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1332
                     /product="protein expanded isoform X2"
                     /calculated_mol_wt=144452
     Region          22..221
                     /region_name="B41"
                     /note="Band 4.1 homologues; smart00295"
                     /db_xref="CDD:214604"
     Region          <22..101
                     /region_name="FERM_F1_PTPN13_like"
                     /note="FERM (Four.1 protein, Ezrin, Radixin, Moesin)
                     domain, F1 sub-domain, found in tyrosine-protein
                     phosphatase non-receptor type 13 (PTPN13) and similar
                     proteins; cd17101"
                     /db_xref="CDD:340621"
     Region          227..328
                     /region_name="FERM_C_FRMD1_FRMD6"
                     /note="FERM domain C-lobe of FERM domain containing 1 and
                     6 proteins; cd13185"
                     /db_xref="CDD:270006"
     Site            order(240,258,260,266)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270006"
     Site            order(270,275..278,308,312,315..316)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     Site            308..319
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     CDS             1..1332
                     /gene="LOC117611804"
                     /coded_by="XM_076690638.1:312..4310"
                     /db_xref="GeneID:117611804"
ORIGIN      
        1 mrekasdaay sildilrrak aksrvkevya qtcmllgqqg mrdcelfgla ilsdgeylfv
       61 dpenklskya pknwrsshty gldssgrpaf vlyfrvryyi dtplllsdet trhhyylqlr
      121 dnvvrhgggv eslhphhplh epsivtpllt laglalqadl gdyseerhrp hagavgyckp
      181 tdylpphmcl esnvlgvlaa qhrdnrglsr eeaelqyire avlleaplna hlyrlrrskn
      241 eagpgrilla icargvrvya eeetprvfaw nnigklcfdr kkfeiravdq pdkltlysgc
      301 ddksklllgl crdthqfsma iaprvneakr reeeerkvlr dcymyparck lsltargarg
      361 dqrisvisst ssnttsgivs drvhsedepd tapasteclp rltenssshp gggggggggc
      421 lvngsnadie drsmpsspas tvdevdgtdn tpttaalrla ssaatgaegs qcssscstvv
      481 vvnapaggpp rmrrtsatss lelgyshtaq nsavssetas fpgaiydrck kagkyagtdi
      541 asetsgvytl rssemqderi gdlyspsgda nessaasdmc alssvqsttd easvtsgfyt
      601 ihsglrsets dvytedvgtd dqepiysvck gdddvandiv vsamsavsld qqledsrsrs
      661 nsilsagsfr gdgsdpssvg pllsadelsd livgrypprk tisnsmdsdc dyvtmppppp
      721 pprtdsdrql pppppypvsm dyatinssra kipdierepp ppppynatrg efvplpprrt
      781 dpppppppyt spreriqipp ptpprndavq preppppppp ypevsdvtrq eaisklypts
      841 steeivsrvt tvtsskfqsn ltnnksnngn itplnsktts siaanhtsnd dvasiapkpp
      901 priqpptypg dkmkptpvha pvaalvvgpn nyldvhasrg gpvllpyltp pppppppppp
      961 mihprqpppp ppslatvyts qvarsqieqy kqqlysdvdy vmfplkdpav skqeyidakq
     1021 gsllaamaay ppppypsfnn kslvmyrstp ylpgssfssp skyasnqnls ssdtssnnyl
     1081 phsnlsshya astsslysga tcssagsqsl rreppapthp htlhafsrtr sdenilkcfd
     1141 spssiklqsl pqlkhrrlpp ppppppyeiq nleknqplps asssssspnk tsktmgteer
     1201 ereegkengm ldirtlreks rnldlplisa lcndryllkq tkafvmpkhp ptettsssts
     1261 akngtrtsni gtssrnkypv sglsttqiak pprksststh khpaevkgna ykvsnstavs
     1321 svkkdptras hs
//
LOCUS       XP_076546755            1253 aa            linear   INV 03-SEP-2025
DEFINITION  protein expanded isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546755
VERSION     XP_076546755.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690640.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1253
                     /product="protein expanded isoform X3"
                     /calculated_mol_wt=135424
     Region          22..142
                     /region_name="FERM_M"
                     /note="FERM central domain; pfam00373"
                     /db_xref="CDD:459788"
     Region          148..249
                     /region_name="FERM_C_FRMD1_FRMD6"
                     /note="FERM domain C-lobe of FERM domain containing 1 and
                     6 proteins; cd13185"
                     /db_xref="CDD:270006"
     Site            order(161,179,181,187)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270006"
     Site            order(191,196..199,229,233,236..237)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     Site            229..240
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     CDS             1..1253
                     /gene="LOC117611804"
                     /coded_by="XM_076690640.1:60..3821"
                     /db_xref="GeneID:117611804"
ORIGIN      
        1 mgldssgrpa fvlyfrvryy idtplllsde ttrhhyylql rdnvvrhggg veslhphhpl
       61 hepsivtpll tlaglalqad lgdyseerhr phagavgyck ptdylpphmc lesnvlgvla
      121 aqhrdnrgls reeaelqyir eavlleapln ahlyrlrrsk neagpgrill aicargvrvy
      181 aeeetprvfa wnnigklcfd rkkfeiravd qpdkltlysg cddksklllg lcrdthqfsm
      241 aiaprvneak rreeeerkvl rdcymyparc klsltargar gdqrisviss tssnttsgiv
      301 sdrvhsedep dtapastecl prltensssh pggggggggg clvngsnadi edrsmpsspa
      361 stvdevdgtd ntpttaalrl assaatgaeg sqcssscstv vvvnapaggp prmrrtsats
      421 slelgyshta qnsavsseta sfpgaiydrc kkagkyagtd iasetsgvyt lrssemqder
      481 igdlyspsgd anessaasdm calssvqstt deasvtsgfy tihsglrset sdvytedvgt
      541 ddqepiysvc kgdddvandi vvsamsavsl dqqledsrsr snsilsagsf rgdgsdpssv
      601 gpllsadels dlivgryppr ktisnsmdsd cdyvtmpppp ppprtdsdrq lpppppypvs
      661 mdyatinssr akipdierep pppppynatr gefvplpprr tdpppppppy tspreriqip
      721 pptpprndav qprepppppp pypevsdvtr qeaisklypt ssteeivsrv ttvtsskfqs
      781 nltnnksnng nitplnsktt ssiaanhtsn ddvasiapkp ppriqpptyp gdkmkptpvh
      841 apvaalvvgp nnyldvhasr ggpvllpylt pppppppppp pmihprqppp pppslatvyt
      901 sqvarsqieq ykqqlysdvd yvmfplkdpa vskqeyidak qgsllaamaa yppppypsfn
      961 nkslvmyrst pylpgssfss pskyasnqnl sssdtssnny lphsnlsshy aastsslysg
     1021 atcssagsqs lrreppapth phtlhafsrt rsdenilkcf dspssiklqs lpqlkhrrlp
     1081 pppppppyei qnleknqplp sasssssspn ktsktmgtee rereegkeng mldirtlrek
     1141 srnldlplis alcndryllk qtkafvmpkh pptettssst sakngtrtsn igtssrnkyp
     1201 vsglsttqia kpprksstst hkhpaevkgn aykvsnstav ssvkkdptra shs
//
LOCUS       XP_076546756            1145 aa            linear   INV 03-SEP-2025
DEFINITION  protein expanded isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546756
VERSION     XP_076546756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690641.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1145
                     /product="protein expanded isoform X4"
                     /calculated_mol_wt=123248
     Region          <11..34
                     /region_name="FERM_M"
                     /note="FERM central domain; pfam00373"
                     /db_xref="CDD:459788"
     Region          40..141
                     /region_name="FERM_C_FRMD1_FRMD6"
                     /note="FERM domain C-lobe of FERM domain containing 1 and
                     6 proteins; cd13185"
                     /db_xref="CDD:270006"
     Site            order(53,71,73,79)
                     /site_type="other"
                     /note="putative phosphoinositide binding site [chemical
                     binding]"
                     /db_xref="CDD:270006"
     Site            order(83,88..91,121,125,128..129)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     Site            121..132
                     /site_type="other"
                     /note="putative actin binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:270006"
     CDS             1..1145
                     /gene="LOC117611804"
                     /coded_by="XM_076690641.1:504..3941"
                     /db_xref="GeneID:117611804"
ORIGIN      
        1 mclesnvlgv laaqhrdnrg lsreeaelqy ireavlleap lnahlyrlrr skneagpgri
       61 llaicargvr vyaeeetprv fawnnigklc fdrkkfeira vdqpdkltly sgcddkskll
      121 lglcrdthqf smaiaprvne akrreeeerk vlrdcymypa rcklsltarg argdqrisvi
      181 sstssnttsg ivsdrvhsed epdtapaste clprltenss shpggggggg ggclvngsna
      241 diedrsmpss pastvdevdg tdntpttaal rlassaatga egsqcssscs tvvvvnapag
      301 gpprmrrtsa tsslelgysh taqnsavsse tasfpgaiyd rckkagkyag tdiasetsgv
      361 ytlrssemqd erigdlysps gdanessaas dmcalssvqs ttdeasvtsg fytihsglrs
      421 etsdvytedv gtddqepiys vckgdddvan divvsamsav sldqqledsr srsnsilsag
      481 sfrgdgsdps svgpllsade lsdlivgryp prktisnsmd sdcdyvtmpp ppppprtdsd
      541 rqlpppppyp vsmdyatins srakipdier eppppppyna trgefvplpp rrtdpppppp
      601 pytspreriq ippptpprnd avqprepppp pppypevsdv trqeaiskly ptssteeivs
      661 rvttvtsskf qsnltnnksn ngnitplnsk ttssiaanht snddvasiap kpppriqppt
      721 ypgdkmkptp vhapvaalvv gpnnyldvha srggpvllpy ltpppppppp pppmihprqp
      781 pppppslatv ytsqvarsqi eqykqqlysd vdyvmfplkd pavskqeyid akqgsllaam
      841 aayppppyps fnnkslvmyr stpylpgssf sspskyasnq nlsssdtssn nylphsnlss
      901 hyaastssly sgatcssags qslrreppap thphtlhafs rtrsdenilk cfdspssikl
      961 qslpqlkhrr lppppppppy eiqnleknqp lpsassssss pnktsktmgt eerereegke
     1021 ngmldirtlr eksrnldlpl isalcndryl lkqtkafvmp khpptettss stsakngtrt
     1081 snigtssrnk ypvsglsttq iakpprksst sthkhpaevk gnaykvsnst avssvkkdpt
     1141 rashs
//
LOCUS       XP_034196002            1293 aa            linear   INV 03-SEP-2025
DEFINITION  putative multidrug resistance-associated protein lethal(2)03659
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196002
VERSION     XP_034196002.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340111.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196002.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1293
                     /product="putative multidrug resistance-associated protein
                     lethal(2)03659 isoform X1"
                     /calculated_mol_wt=147337
     Region          4..1270
                     /region_name="MRP_assoc_pro"
                     /note="multi drug resistance-associated protein (MRP);
                     TIGR00957"
                     /db_xref="CDD:188098"
     CDS             1..1293
                     /gene="LOC117611805"
                     /coded_by="XM_034340111.2:621..4502"
                     /db_xref="GeneID:117611805"
ORIGIN      
        1 mdksekkevk npcenanpls iltfwwilkl fiigykkelk eedlysplke dkssylgqri
       61 vknweievkr hekrkdspkp slfrvlykcf gkavmntgla lfvlefgiri vnpfllakll
      121 ryfsgnrkdw tsdvyyyaaa fsllplldav ilhwslqnlm hvgmkvrvac ctliyrkilk
      181 lsnavlenet sagqmvnfls ndvnrldyfv fsihylwigp lqvflisyli yreiglgait
      241 gmttfllcip lqlyfgkkvs rltlvsaqkt dnrlrlmnqi iagveiikmy vwevpysllv
      301 ekarrkevdv ikkysiveql gltfdiyvpr vslfitilty vltgntidae kvfmitafyt
      361 ilrssmtvgf alsvhqlaea mvsirrlekf mmhpeiaapp kvqnqvatqs lpvylknvta
      421 rwdetrdytl qnvdltirag sfvavigqig sgkssllqai lgelplqegt lesygkirfa
      481 dqrpwifass irqnilfgep mnekrynqvi nvcqlrtdid tfthkdrtmv gergmnlsgg
      541 qrarinlara vyadadiyll ddplsavdsh vgsrivdeci cgflrdktri lvthqiqylk
      601 sadqiivmnn giiqakgsfe elqrmsldfm kifkeveenk knkepevisd erqtiveeet
      661 kkeeepavsd eepvelaetr tvgrisstvf lsywkasrnl cllvlmvilf iasqimasgs
      721 dyllafwvnt eaaswfkten gtrtfqwngs lsrdgiiylw sgltmgvvlv ymfqtftyyg
      781 vcmrasknlh aqmfrsivra amyfyntnpa grilnrfskd igvidkklpf tmfdvtimfl
      841 tfigtivivg tvsvwllipt fivillfyym rviyistsrs vkrmegttrs pvfdhvgatl
      901 qglttirafk aekvvtadfd nhqdlhtstw fifisisraf glyiewfcli yvglitvifl
      961 lfddlavagn iglvitqita itgilqwgmr qtaelenhmt siervleysn ldeepfldst
     1021 pdkkppndwp nqglvefknv klkygpkspy vlkginfvvk akekvgvvgr tgagktslis
     1081 alfrlayieg eiivddiptd rvalhdfrlk isiipqepvl fggslrrnld pfdeysdndl
     1141 waalqevelk esisemaagl nskvaeegsn fsvgqrqllc lvralvrnnk imvldeatan
     1201 vdpqtdsliq ktvrkkfmhc tvftiahrln timdsdkilv mdqghlveyd hpyvllqrkg
     1261 yfynmvqetg stmayslsei aknafyknnq vss
//
LOCUS       XP_034196003            1293 aa            linear   INV 03-SEP-2025
DEFINITION  putative multidrug resistance-associated protein lethal(2)03659
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196003
VERSION     XP_034196003.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340112.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196003.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1293
                     /product="putative multidrug resistance-associated protein
                     lethal(2)03659 isoform X1"
                     /calculated_mol_wt=147337
     Region          4..1270
                     /region_name="MRP_assoc_pro"
                     /note="multi drug resistance-associated protein (MRP);
                     TIGR00957"
                     /db_xref="CDD:188098"
     CDS             1..1293
                     /gene="LOC117611805"
                     /coded_by="XM_034340112.2:191..4072"
                     /db_xref="GeneID:117611805"
ORIGIN      
        1 mdksekkevk npcenanpls iltfwwilkl fiigykkelk eedlysplke dkssylgqri
       61 vknweievkr hekrkdspkp slfrvlykcf gkavmntgla lfvlefgiri vnpfllakll
      121 ryfsgnrkdw tsdvyyyaaa fsllplldav ilhwslqnlm hvgmkvrvac ctliyrkilk
      181 lsnavlenet sagqmvnfls ndvnrldyfv fsihylwigp lqvflisyli yreiglgait
      241 gmttfllcip lqlyfgkkvs rltlvsaqkt dnrlrlmnqi iagveiikmy vwevpysllv
      301 ekarrkevdv ikkysiveql gltfdiyvpr vslfitilty vltgntidae kvfmitafyt
      361 ilrssmtvgf alsvhqlaea mvsirrlekf mmhpeiaapp kvqnqvatqs lpvylknvta
      421 rwdetrdytl qnvdltirag sfvavigqig sgkssllqai lgelplqegt lesygkirfa
      481 dqrpwifass irqnilfgep mnekrynqvi nvcqlrtdid tfthkdrtmv gergmnlsgg
      541 qrarinlara vyadadiyll ddplsavdsh vgsrivdeci cgflrdktri lvthqiqylk
      601 sadqiivmnn giiqakgsfe elqrmsldfm kifkeveenk knkepevisd erqtiveeet
      661 kkeeepavsd eepvelaetr tvgrisstvf lsywkasrnl cllvlmvilf iasqimasgs
      721 dyllafwvnt eaaswfkten gtrtfqwngs lsrdgiiylw sgltmgvvlv ymfqtftyyg
      781 vcmrasknlh aqmfrsivra amyfyntnpa grilnrfskd igvidkklpf tmfdvtimfl
      841 tfigtivivg tvsvwllipt fivillfyym rviyistsrs vkrmegttrs pvfdhvgatl
      901 qglttirafk aekvvtadfd nhqdlhtstw fifisisraf glyiewfcli yvglitvifl
      961 lfddlavagn iglvitqita itgilqwgmr qtaelenhmt siervleysn ldeepfldst
     1021 pdkkppndwp nqglvefknv klkygpkspy vlkginfvvk akekvgvvgr tgagktslis
     1081 alfrlayieg eiivddiptd rvalhdfrlk isiipqepvl fggslrrnld pfdeysdndl
     1141 waalqevelk esisemaagl nskvaeegsn fsvgqrqllc lvralvrnnk imvldeatan
     1201 vdpqtdsliq ktvrkkfmhc tvftiahrln timdsdkilv mdqghlveyd hpyvllqrkg
     1261 yfynmvqetg stmayslsei aknafyknnq vss
//
LOCUS       XP_034196004            1293 aa            linear   INV 03-SEP-2025
DEFINITION  putative multidrug resistance-associated protein lethal(2)03659
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196004
VERSION     XP_034196004.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340113.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196004.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1293
                     /product="putative multidrug resistance-associated protein
                     lethal(2)03659 isoform X1"
                     /calculated_mol_wt=147337
     Region          4..1270
                     /region_name="MRP_assoc_pro"
                     /note="multi drug resistance-associated protein (MRP);
                     TIGR00957"
                     /db_xref="CDD:188098"
     CDS             1..1293
                     /gene="LOC117611805"
                     /coded_by="XM_034340113.2:207..4088"
                     /db_xref="GeneID:117611805"
ORIGIN      
        1 mdksekkevk npcenanpls iltfwwilkl fiigykkelk eedlysplke dkssylgqri
       61 vknweievkr hekrkdspkp slfrvlykcf gkavmntgla lfvlefgiri vnpfllakll
      121 ryfsgnrkdw tsdvyyyaaa fsllplldav ilhwslqnlm hvgmkvrvac ctliyrkilk
      181 lsnavlenet sagqmvnfls ndvnrldyfv fsihylwigp lqvflisyli yreiglgait
      241 gmttfllcip lqlyfgkkvs rltlvsaqkt dnrlrlmnqi iagveiikmy vwevpysllv
      301 ekarrkevdv ikkysiveql gltfdiyvpr vslfitilty vltgntidae kvfmitafyt
      361 ilrssmtvgf alsvhqlaea mvsirrlekf mmhpeiaapp kvqnqvatqs lpvylknvta
      421 rwdetrdytl qnvdltirag sfvavigqig sgkssllqai lgelplqegt lesygkirfa
      481 dqrpwifass irqnilfgep mnekrynqvi nvcqlrtdid tfthkdrtmv gergmnlsgg
      541 qrarinlara vyadadiyll ddplsavdsh vgsrivdeci cgflrdktri lvthqiqylk
      601 sadqiivmnn giiqakgsfe elqrmsldfm kifkeveenk knkepevisd erqtiveeet
      661 kkeeepavsd eepvelaetr tvgrisstvf lsywkasrnl cllvlmvilf iasqimasgs
      721 dyllafwvnt eaaswfkten gtrtfqwngs lsrdgiiylw sgltmgvvlv ymfqtftyyg
      781 vcmrasknlh aqmfrsivra amyfyntnpa grilnrfskd igvidkklpf tmfdvtimfl
      841 tfigtivivg tvsvwllipt fivillfyym rviyistsrs vkrmegttrs pvfdhvgatl
      901 qglttirafk aekvvtadfd nhqdlhtstw fifisisraf glyiewfcli yvglitvifl
      961 lfddlavagn iglvitqita itgilqwgmr qtaelenhmt siervleysn ldeepfldst
     1021 pdkkppndwp nqglvefknv klkygpkspy vlkginfvvk akekvgvvgr tgagktslis
     1081 alfrlayieg eiivddiptd rvalhdfrlk isiipqepvl fggslrrnld pfdeysdndl
     1141 waalqevelk esisemaagl nskvaeegsn fsvgqrqllc lvralvrnnk imvldeatan
     1201 vdpqtdsliq ktvrkkfmhc tvftiahrln timdsdkilv mdqghlveyd hpyvllqrkg
     1261 yfynmvqetg stmayslsei aknafyknnq vss
//
LOCUS       XP_034196006            1293 aa            linear   INV 03-SEP-2025
DEFINITION  putative multidrug resistance-associated protein lethal(2)03659
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196006
VERSION     XP_034196006.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340115.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196006.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1293
                     /product="putative multidrug resistance-associated protein
                     lethal(2)03659 isoform X1"
                     /calculated_mol_wt=147337
     Region          4..1270
                     /region_name="MRP_assoc_pro"
                     /note="multi drug resistance-associated protein (MRP);
                     TIGR00957"
                     /db_xref="CDD:188098"
     CDS             1..1293
                     /gene="LOC117611805"
                     /coded_by="XM_034340115.2:211..4092"
                     /db_xref="GeneID:117611805"
ORIGIN      
        1 mdksekkevk npcenanpls iltfwwilkl fiigykkelk eedlysplke dkssylgqri
       61 vknweievkr hekrkdspkp slfrvlykcf gkavmntgla lfvlefgiri vnpfllakll
      121 ryfsgnrkdw tsdvyyyaaa fsllplldav ilhwslqnlm hvgmkvrvac ctliyrkilk
      181 lsnavlenet sagqmvnfls ndvnrldyfv fsihylwigp lqvflisyli yreiglgait
      241 gmttfllcip lqlyfgkkvs rltlvsaqkt dnrlrlmnqi iagveiikmy vwevpysllv
      301 ekarrkevdv ikkysiveql gltfdiyvpr vslfitilty vltgntidae kvfmitafyt
      361 ilrssmtvgf alsvhqlaea mvsirrlekf mmhpeiaapp kvqnqvatqs lpvylknvta
      421 rwdetrdytl qnvdltirag sfvavigqig sgkssllqai lgelplqegt lesygkirfa
      481 dqrpwifass irqnilfgep mnekrynqvi nvcqlrtdid tfthkdrtmv gergmnlsgg
      541 qrarinlara vyadadiyll ddplsavdsh vgsrivdeci cgflrdktri lvthqiqylk
      601 sadqiivmnn giiqakgsfe elqrmsldfm kifkeveenk knkepevisd erqtiveeet
      661 kkeeepavsd eepvelaetr tvgrisstvf lsywkasrnl cllvlmvilf iasqimasgs
      721 dyllafwvnt eaaswfkten gtrtfqwngs lsrdgiiylw sgltmgvvlv ymfqtftyyg
      781 vcmrasknlh aqmfrsivra amyfyntnpa grilnrfskd igvidkklpf tmfdvtimfl
      841 tfigtivivg tvsvwllipt fivillfyym rviyistsrs vkrmegttrs pvfdhvgatl
      901 qglttirafk aekvvtadfd nhqdlhtstw fifisisraf glyiewfcli yvglitvifl
      961 lfddlavagn iglvitqita itgilqwgmr qtaelenhmt siervleysn ldeepfldst
     1021 pdkkppndwp nqglvefknv klkygpkspy vlkginfvvk akekvgvvgr tgagktslis
     1081 alfrlayieg eiivddiptd rvalhdfrlk isiipqepvl fggslrrnld pfdeysdndl
     1141 waalqevelk esisemaagl nskvaeegsn fsvgqrqllc lvralvrnnk imvldeatan
     1201 vdpqtdsliq ktvrkkfmhc tvftiahrln timdsdkilv mdqghlveyd hpyvllqrkg
     1261 yfynmvqetg stmayslsei aknafyknnq vss
//
LOCUS       XP_034196007            1293 aa            linear   INV 03-SEP-2025
DEFINITION  putative multidrug resistance-associated protein lethal(2)03659
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196007
VERSION     XP_034196007.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340116.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196007.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1293
                     /product="putative multidrug resistance-associated protein
                     lethal(2)03659 isoform X1"
                     /calculated_mol_wt=147337
     Region          4..1270
                     /region_name="MRP_assoc_pro"
                     /note="multi drug resistance-associated protein (MRP);
                     TIGR00957"
                     /db_xref="CDD:188098"
     CDS             1..1293
                     /gene="LOC117611805"
                     /coded_by="XM_034340116.2:89..3970"
                     /db_xref="GeneID:117611805"
ORIGIN      
        1 mdksekkevk npcenanpls iltfwwilkl fiigykkelk eedlysplke dkssylgqri
       61 vknweievkr hekrkdspkp slfrvlykcf gkavmntgla lfvlefgiri vnpfllakll
      121 ryfsgnrkdw tsdvyyyaaa fsllplldav ilhwslqnlm hvgmkvrvac ctliyrkilk
      181 lsnavlenet sagqmvnfls ndvnrldyfv fsihylwigp lqvflisyli yreiglgait
      241 gmttfllcip lqlyfgkkvs rltlvsaqkt dnrlrlmnqi iagveiikmy vwevpysllv
      301 ekarrkevdv ikkysiveql gltfdiyvpr vslfitilty vltgntidae kvfmitafyt
      361 ilrssmtvgf alsvhqlaea mvsirrlekf mmhpeiaapp kvqnqvatqs lpvylknvta
      421 rwdetrdytl qnvdltirag sfvavigqig sgkssllqai lgelplqegt lesygkirfa
      481 dqrpwifass irqnilfgep mnekrynqvi nvcqlrtdid tfthkdrtmv gergmnlsgg
      541 qrarinlara vyadadiyll ddplsavdsh vgsrivdeci cgflrdktri lvthqiqylk
      601 sadqiivmnn giiqakgsfe elqrmsldfm kifkeveenk knkepevisd erqtiveeet
      661 kkeeepavsd eepvelaetr tvgrisstvf lsywkasrnl cllvlmvilf iasqimasgs
      721 dyllafwvnt eaaswfkten gtrtfqwngs lsrdgiiylw sgltmgvvlv ymfqtftyyg
      781 vcmrasknlh aqmfrsivra amyfyntnpa grilnrfskd igvidkklpf tmfdvtimfl
      841 tfigtivivg tvsvwllipt fivillfyym rviyistsrs vkrmegttrs pvfdhvgatl
      901 qglttirafk aekvvtadfd nhqdlhtstw fifisisraf glyiewfcli yvglitvifl
      961 lfddlavagn iglvitqita itgilqwgmr qtaelenhmt siervleysn ldeepfldst
     1021 pdkkppndwp nqglvefknv klkygpkspy vlkginfvvk akekvgvvgr tgagktslis
     1081 alfrlayieg eiivddiptd rvalhdfrlk isiipqepvl fggslrrnld pfdeysdndl
     1141 waalqevelk esisemaagl nskvaeegsn fsvgqrqllc lvralvrnnk imvldeatan
     1201 vdpqtdsliq ktvrkkfmhc tvftiahrln timdsdkilv mdqghlveyd hpyvllqrkg
     1261 yfynmvqetg stmayslsei aknafyknnq vss
//
LOCUS       XP_034196008            1187 aa            linear   INV 03-SEP-2025
DEFINITION  putative multidrug resistance-associated protein lethal(2)03659
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196008
VERSION     XP_034196008.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340117.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196008.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1187
                     /product="putative multidrug resistance-associated protein
                     lethal(2)03659 isoform X2"
                     /calculated_mol_wt=134844
     Region          <1..1164
                     /region_name="MRP_assoc_pro"
                     /note="multi drug resistance-associated protein (MRP);
                     TIGR00957"
                     /db_xref="CDD:188098"
     CDS             1..1187
                     /gene="LOC117611805"
                     /coded_by="XM_034340117.2:129..3692"
                     /db_xref="GeneID:117611805"
ORIGIN      
        1 mlrivnpfll akllryfsgn rkdwtsdvyy yaaafsllpl ldavilhwsl qnlmhvgmkv
       61 rvacctliyr kilklsnavl enetsagqmv nflsndvnrl dyfvfsihyl wigplqvfli
      121 syliyreigl gaitgmttfl lciplqlyfg kkvsrltlvs aqktdnrlrl mnqiiagvei
      181 ikmyvwevpy sllvekarrk evdvikkysi veqlgltfdi yvprvslfit iltyvltgnt
      241 idaekvfmit afytilrssm tvgfalsvhq laeamvsirr lekfmmhpei aappkvqnqv
      301 atqslpvylk nvtarwdetr dytlqnvdlt iragsfvavi gqigsgkssl lqailgelpl
      361 qegtlesygk irfadqrpwi fassirqnil fgepmnekry nqvinvcqlr tdidtfthkd
      421 rtmvgergmn lsggqrarin laravyadad iyllddplsa vdshvgsriv decicgflrd
      481 ktrilvthqi qylksadqii vmnngiiqak gsfeelqrms ldfmkifkev eenkknkepe
      541 visderqtiv eeetkkeeep avsdeepvel aetrtvgris stvflsywka srnlcllvlm
      601 vilfiasqim asgsdyllaf wvnteaaswf ktengtrtfq wngslsrdgi iylwsgltmg
      661 vvlvymfqtf tyygvcmras knlhaqmfrs ivraamyfyn tnpagrilnr fskdigvidk
      721 klpftmfdvt imfltfigti vivgtvsvwl liptfivill fyymrviyis tsrsvkrmeg
      781 ttrspvfdhv gatlqgltti rafkaekvvt adfdnhqdlh tstwfifisi srafglyiew
      841 fcliyvglit vifllfddla vagniglvit qitaitgilq wgmrqtaele nhmtsiervl
      901 eysnldeepf ldstpdkkpp ndwpnqglve fknvklkygp kspyvlkgin fvvkakekvg
      961 vvgrtgagkt slisalfrla yiegeiivdd iptdrvalhd frlkisiipq epvlfggslr
     1021 rnldpfdeys dndlwaalqe velkesisem aaglnskvae egsnfsvgqr qllclvralv
     1081 rnnkimvlde atanvdpqtd sliqktvrkk fmhctvftia hrlntimdsd kilvmdqghl
     1141 veydhpyvll qrkgyfynmv qetgstmays lseiaknafy knnqvss
//
LOCUS       XP_076546757             735 aa            linear   INV 03-SEP-2025
DEFINITION  maltase A2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546757
VERSION     XP_076546757.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690642.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..735
                     /product="maltase A2 isoform X1"
                     /calculated_mol_wt=81540
     Region          229..644
                     /region_name="AmyAc_family"
                     /note="Alpha amylase catalytic domain family; cl38930"
                     /db_xref="CDD:476817"
     Site            order(336,438,440,471,535..536)
                     /site_type="active"
                     /db_xref="CDD:200451"
     Site            order(440,471,536)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:200451"
     CDS             1..735
                     /gene="LOC117611807"
                     /coded_by="XM_076690642.1:144..2351"
                     /db_xref="GeneID:117611807"
ORIGIN      
        1 mniqkpegll svvlptelas svssrqliin qdqqvcsrqt iqdeeasdcp lltpsppgve
       61 psnalesgai fvslegnpve sippptleni nkedglnefm nivnsssrvl ndepssrdpm
      121 vestssgsss dtngpvcaql ltqlntayqh lapdaqalfy nqenggkppl vgiqlvvpkp
      181 skdyrfmkwn wplirktcfw slmsvlagct alvigviatm prkcdptvqw wqgsvfyeif
      241 pasfqdsskg gdgigdirgi tmrldylkel girgirlnsi fpaahypeyy snidnltdln
      301 ehlgtlddfa tlvrqvhrrn mtilldlply pfvktlvedd asvgkinkte kavrfererr
      361 dvtdnddndd ddpvlkdiap ttpsmsvqai realssippp ldqsqhealf snplkavten
      421 svtiairtwl qrgvdgfylk glehyvdeds fasnlkhwks ilgserilic hvnalnaakt
      481 vasrnsiltr idlldvtlrv angtkeikrq veditrgvlf ekagypwvhw siggvdsari
      541 astisvknat maatllgmml pgtpsifygd eigivdcece dhkdlshvhn lapmyweknd
      601 gsgnkfasig vtawlpeatk pletslmgti aemadlrsqt tpiyvkavlk qnqvlancdi
      661 ryaddemivi erwyprrnsy vfvanlgnet qtkdlsflyy gghvvvgpty rlnrdvyfke
      721 liippgeafv ikldk
//
LOCUS       XP_076546758             731 aa            linear   INV 03-SEP-2025
DEFINITION  maltase A2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546758
VERSION     XP_076546758.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690643.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..731
                     /product="maltase A2 isoform X2"
                     /calculated_mol_wt=80987
     Region          225..640
                     /region_name="AmyAc_family"
                     /note="Alpha amylase catalytic domain family; cl38930"
                     /db_xref="CDD:476817"
     Site            order(332,434,436,467,531..532)
                     /site_type="active"
                     /db_xref="CDD:200451"
     Site            order(436,467,532)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:200451"
     CDS             1..731
                     /gene="LOC117611807"
                     /coded_by="XM_076690643.1:144..2339"
                     /db_xref="GeneID:117611807"
ORIGIN      
        1 mniqkpegll svvlptelas svssrqliin qdqqvcsrqt iqdeeasdcp lltpsppgve
       61 psnalesgai fvslegnpve sippptleni nkedglnefm nivnsssrvl ndepssrdpm
      121 vestssgsss dtngpvcaql ltqlntayqh lapdaqalen ggkpplvgiq lvvpkpskdy
      181 rfmkwnwpli rktcfwslms vlagctalvi gviatmprkc dptvqwwqgs vfyeifpasf
      241 qdsskggdgi gdirgitmrl dylkelgirg irlnsifpaa hypeyysnid nltdlnehlg
      301 tlddfatlvr qvhrrnmtil ldlplypfvk tlveddasvg kinktekavr fererrdvtd
      361 nddnddddpv lkdiapttps msvqaireal ssipppldqs qhealfsnpl kavtensvti
      421 airtwlqrgv dgfylkgleh yvdedsfasn lkhwksilgs erilichvna lnaaktvasr
      481 nsiltridll dvtlrvangt keikrqvedi trgvlfekag ypwvhwsigg vdsariasti
      541 svknatmaat llgmmlpgtp sifygdeigi vdcecedhkd lshvhnlapm ywekndgsgn
      601 kfasigvtaw lpeatkplet slmgtiaema dlrsqttpiy vkavlkqnqv lancdiryad
      661 demivierwy prrnsyvfva nlgnetqtkd lsflyygghv vvgptyrlnr dvyfkeliip
      721 pgeafvikld k
//
LOCUS       XP_034196009             727 aa            linear   INV 03-SEP-2025
DEFINITION  maltase A2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196009
VERSION     XP_034196009.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340118.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..727
                     /product="maltase A2 isoform X3"
                     /calculated_mol_wt=80624
     Region          221..636
                     /region_name="AmyAc_family"
                     /note="Alpha amylase catalytic domain family; cl38930"
                     /db_xref="CDD:476817"
     Site            order(328,430,432,463,527..528)
                     /site_type="active"
                     /db_xref="CDD:200451"
     Site            order(432,463,528)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:200451"
     CDS             1..727
                     /gene="LOC117611807"
                     /coded_by="XM_034340118.2:144..2327"
                     /db_xref="GeneID:117611807"
ORIGIN      
        1 mniqkpegll svvlptelas svssrqliin qdqqdeeasd cplltpsppg vepsnalesg
       61 aifvslegnp vesippptle ninkedglne fmnivnsssr vlndepssrd pmvestssgs
      121 ssdtngpvca qlltqlntay qhlapdaqal fynqenggkp plvgiqlvvp kpskdyrfmk
      181 wnwplirktc fwslmsvlag ctalvigvia tmprkcdptv qwwqgsvfye ifpasfqdss
      241 kggdgigdir gitmrldylk elgirgirln sifpaahype yysnidnltd lnehlgtldd
      301 fatlvrqvhr rnmtilldlp lypfvktlve ddasvgkink tekavrfere rrdvtdnddn
      361 ddddpvlkdi apttpsmsvq airealssip ppldqsqhea lfsnplkavt ensvtiairt
      421 wlqrgvdgfy lkglehyvde dsfasnlkhw ksilgseril ichvnalnaa ktvasrnsil
      481 tridlldvtl rvangtkeik rqveditrgv lfekagypwv hwsiggvdsa riastisvkn
      541 atmaatllgm mlpgtpsify gdeigivdce cedhkdlshv hnlapmywek ndgsgnkfas
      601 igvtawlpea tkpletslmg tiaemadlrs qttpiyvkav lkqnqvlanc diryaddemi
      661 vierwyprrn syvfvanlgn etqtkdlsfl yygghvvvgp tyrlnrdvyf keliippgea
      721 fvikldk
//
LOCUS       XP_034196011             636 aa            linear   INV 03-SEP-2025
DEFINITION  maltase A2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196011
VERSION     XP_034196011.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340120.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="maltase A2 isoform X4"
                     /calculated_mol_wt=70945
     Region          130..545
                     /region_name="AmyAc_family"
                     /note="Alpha amylase catalytic domain family; cl38930"
                     /db_xref="CDD:476817"
     Site            order(237,339,341,372,436..437)
                     /site_type="active"
                     /db_xref="CDD:200451"
     Site            order(341,372,437)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:200451"
     CDS             1..636
                     /gene="LOC117611807"
                     /coded_by="XM_034340120.2:311..2221"
                     /db_xref="GeneID:117611807"
ORIGIN      
        1 mnivnsssrv lndepssrdp mvestssgss sdtngpvcaq lltqlntayq hlapdaqalf
       61 ynqenggkpp lvgiqlvvpk pskdyrfmkw nwplirktcf wslmsvlagc talvigviat
      121 mprkcdptvq wwqgsvfyei fpasfqdssk ggdgigdirg itmrldylke lgirgirlns
      181 ifpaahypey ysnidnltdl nehlgtlddf atlvrqvhrr nmtilldlpl ypfvktlved
      241 dasvgkinkt ekavrferer rdvtdnddnd dddpvlkdia pttpsmsvqa irealssipp
      301 pldqsqheal fsnplkavte nsvtiairtw lqrgvdgfyl kglehyvded sfasnlkhwk
      361 silgserili chvnalnaak tvasrnsilt ridlldvtlr vangtkeikr qveditrgvl
      421 fekagypwvh wsiggvdsar iastisvkna tmaatllgmm lpgtpsifyg deigivdcec
      481 edhkdlshvh nlapmywekn dgsgnkfasi gvtawlpeat kpletslmgt iaemadlrsq
      541 ttpiyvkavl kqnqvlancd iryaddemiv ierwyprrns yvfvanlgne tqtkdlsfly
      601 ygghvvvgpt yrlnrdvyfk eliippgeaf vikldk
//
LOCUS       XP_034196013             568 aa            linear   INV 03-SEP-2025
DEFINITION  putative sodium-dependent multivitamin transporter [Osmia lignaria
            lignaria].
ACCESSION   XP_034196013
VERSION     XP_034196013.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196013.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..568
                     /product="putative sodium-dependent multivitamin
                     transporter"
                     /calculated_mol_wt=62613
     Region          7..527
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(57,60,342,345..346)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..568
                     /gene="LOC117611808"
                     /coded_by="XM_034340122.2:240..1946"
                     /db_xref="GeneID:117611808"
ORIGIN      
        1 mitlgtvdya vivltmlist gigvyywrtg gkeksteeyf vanrsmrvap vaialtvsyl
       61 savsllgvss enylygsqya vinvgyglat pivayfympv ffnlgsasaf eylqqrfgta
      121 arlagsfafl lqlllysgvv lyapalalea ttgisttasv iliglvctfy stiggikavl
      181 itdvfqsilm liavivvivt eavdvgglde iweiardgsr iefdnismdp tvrhtwwslt
      241 fggfftylsl fgsnqvqvqr mltirnlkda rsaiwwswpl ssimslglcf cglaiyaeyr
      301 ncdpleigri tsydqlmply vmdmlstypg ipglfiagif saglstisat vnslaavile
      361 dfirpvcrtr gkdmsraesm viskilaliv gvaciavafm srylggllqa altifgvvgg
      421 pvlglftlgm ftergnqpga viglmislif slwigfgqpk ppipnkvqat ecnvtfyhdy
      481 eeslnlhfqn gesnddsyfy lyrisymwyc pigsvlsfga gwifswifdr iskeerkeln
      541 pdlftpilas rvrkrrskyi nefhsnis
//
LOCUS       XP_076546759             121 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305774 [Osmia lignaria lignaria].
ACCESSION   XP_076546759
VERSION     XP_076546759.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690644.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..121
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..121
                     /product="uncharacterized protein LOC143305774"
                     /calculated_mol_wt=13782
     CDS             1..121
                     /gene="LOC143305774"
                     /coded_by="XM_076690644.1:543..908"
                     /db_xref="GeneID:143305774"
ORIGIN      
        1 mellainqrt ndsglsskls ivvneaidtm kngillyglk qrlhnettag hsvtkfdilr
       61 vvvgceilmn rdmshntlkh vetqfehgch iyqlcttwht vvrllienfy krsrtsnnfl
      121 p
//
LOCUS       XP_034196014             105 aa            linear   INV 03-SEP-2025
DEFINITION  thioredoxin-2 [Osmia lignaria lignaria].
ACCESSION   XP_034196014
VERSION     XP_034196014.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340123.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="thioredoxin-2"
                     /calculated_mol_wt=11814
     Region          9..93
                     /region_name="TRX_family"
                     /note="TRX family; composed of two groups: Group I, which
                     includes proteins that exclusively encode a TRX domain;
                     and Group II, which are composed of fusion proteins of TRX
                     and additional domains. Group I TRX is a small ancient
                     protein that alter the redox...; cd02947"
                     /db_xref="CDD:239245"
     Site            order(32,35)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239245"
     CDS             1..105
                     /gene="LOC117611809"
                     /coded_by="XM_034340123.2:144..461"
                     /db_xref="GeneID:117611809"
ORIGIN      
        1 mvveiesved lkskiekagd qlividfyat wcgpckmiap kieelskemq dvifvkvdvd
       61 vrediatdyd insmptfvfi kngkvvesfa ganydkvkdt ilkyk
//
LOCUS       XP_034195988             459 aa            linear   INV 03-SEP-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195988
VERSION     XP_034195988.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340097.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="misexpression suppressor of KSR 2 isoform X1"
                     /calculated_mol_wt=51103
     Region          124..413
                     /region_name="Ndr"
                     /note="Ndr family; pfam03096"
                     /db_xref="CDD:397285"
     CDS             1..459
                     /gene="MESK2"
                     /coded_by="XM_034340097.2:567..1946"
                     /db_xref="GeneID:117611798"
ORIGIN      
        1 mpagigasyr slgdlgenvy ksttivdqtq ttgqsvlesv kkafsfaspk velrkkdpli
       61 edrresvsiv srernrmpta ataeesallg tmpsdsmddi elkniqlqfp alrylsrddn
      121 svreervetd kgsllvavqg nrakpailty hdlglnyiss fqaffnyidm rvllenfcvy
      181 hvnapgqeeg aptlpedyvy psmdelaeql lfvlghfglk svigfgvgag anilarfala
      241 hpekvnalcl incvstqagw iewgyqklnv rhlrsqgmtq gvldylmwhh fgrgteernh
      301 dlvqvyknyf errvnptnla lfidsyvrrt dlnitreldp trkkegltlg vpvmnitgal
      361 sphvddtvtl ngrldpanss wmkisdcgmv leeqpgkvse afrlflqgeg yvaplsplkm
      421 adyrlasleg lnhvcskkid wptatihite npiseavvc
//
LOCUS       XP_034195989             459 aa            linear   INV 03-SEP-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195989
VERSION     XP_034195989.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="misexpression suppressor of KSR 2 isoform X1"
                     /calculated_mol_wt=51103
     Region          124..413
                     /region_name="Ndr"
                     /note="Ndr family; pfam03096"
                     /db_xref="CDD:397285"
     CDS             1..459
                     /gene="MESK2"
                     /coded_by="XM_034340098.2:527..1906"
                     /db_xref="GeneID:117611798"
ORIGIN      
        1 mpagigasyr slgdlgenvy ksttivdqtq ttgqsvlesv kkafsfaspk velrkkdpli
       61 edrresvsiv srernrmpta ataeesallg tmpsdsmddi elkniqlqfp alrylsrddn
      121 svreervetd kgsllvavqg nrakpailty hdlglnyiss fqaffnyidm rvllenfcvy
      181 hvnapgqeeg aptlpedyvy psmdelaeql lfvlghfglk svigfgvgag anilarfala
      241 hpekvnalcl incvstqagw iewgyqklnv rhlrsqgmtq gvldylmwhh fgrgteernh
      301 dlvqvyknyf errvnptnla lfidsyvrrt dlnitreldp trkkegltlg vpvmnitgal
      361 sphvddtvtl ngrldpanss wmkisdcgmv leeqpgkvse afrlflqgeg yvaplsplkm
      421 adyrlasleg lnhvcskkid wptatihite npiseavvc
//
LOCUS       XP_034195990             459 aa            linear   INV 03-SEP-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195990
VERSION     XP_034195990.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340099.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="misexpression suppressor of KSR 2 isoform X1"
                     /calculated_mol_wt=51103
     Region          124..413
                     /region_name="Ndr"
                     /note="Ndr family; pfam03096"
                     /db_xref="CDD:397285"
     CDS             1..459
                     /gene="MESK2"
                     /coded_by="XM_034340099.2:561..1940"
                     /db_xref="GeneID:117611798"
ORIGIN      
        1 mpagigasyr slgdlgenvy ksttivdqtq ttgqsvlesv kkafsfaspk velrkkdpli
       61 edrresvsiv srernrmpta ataeesallg tmpsdsmddi elkniqlqfp alrylsrddn
      121 svreervetd kgsllvavqg nrakpailty hdlglnyiss fqaffnyidm rvllenfcvy
      181 hvnapgqeeg aptlpedyvy psmdelaeql lfvlghfglk svigfgvgag anilarfala
      241 hpekvnalcl incvstqagw iewgyqklnv rhlrsqgmtq gvldylmwhh fgrgteernh
      301 dlvqvyknyf errvnptnla lfidsyvrrt dlnitreldp trkkegltlg vpvmnitgal
      361 sphvddtvtl ngrldpanss wmkisdcgmv leeqpgkvse afrlflqgeg yvaplsplkm
      421 adyrlasleg lnhvcskkid wptatihite npiseavvc
//
LOCUS       XP_034195992             441 aa            linear   INV 03-SEP-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195992
VERSION     XP_034195992.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340101.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="misexpression suppressor of KSR 2 isoform X2"
                     /calculated_mol_wt=49135
     Region          106..395
                     /region_name="Ndr"
                     /note="Ndr family; pfam03096"
                     /db_xref="CDD:397285"
     CDS             1..441
                     /gene="MESK2"
                     /coded_by="XM_034340101.2:567..1892"
                     /db_xref="GeneID:117611798"
ORIGIN      
        1 mpagigasyr slgdlgenvy ksttivdqtq ttgqsvlesv kkafsfaspk velrkkdpli
       61 edrresvsiv sstmpsdsmd dielkniqlq fpalrylsrd dnsvreerve tdkgsllvav
      121 qgnrakpail tyhdlglnyi ssfqaffnyi dmrvllenfc vyhvnapgqe egaptlpedy
      181 vypsmdelae qllfvlghfg lksvigfgvg aganilarfa lahpekvnal clincvstqa
      241 gwiewgyqkl nvrhlrsqgm tqgvldylmw hhfgrgteer nhdlvqvykn yferrvnptn
      301 lalfidsyvr rtdlnitrel dptrkkeglt lgvpvmnitg alsphvddtv tlngrldpan
      361 sswmkisdcg mvleeqpgkv seafrlflqg egyvaplspl kmadyrlasl eglnhvcskk
      421 idwptatihi tenpiseavv c
//
LOCUS       XP_034195993             383 aa            linear   INV 03-SEP-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195993
VERSION     XP_034195993.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340102.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..383
                     /product="misexpression suppressor of KSR 2 isoform X3"
                     /calculated_mol_wt=42692
     Region          48..337
                     /region_name="alpha/beta hydrolases"
                     /note="A functionally diverse superfamily containing
                     proteases, lipases, peroxidases, esterases, epoxide
                     hydrolases and dehalogenases. The catalytic apparatus
                     typically involves three residues (catalytic triad): a
                     serine, a glutamate or aspartate and a...; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..383
                     /gene="MESK2"
                     /coded_by="XM_034340102.2:408..1559"
                     /db_xref="GeneID:117611798"
ORIGIN      
        1 mptaataees allgtmpsds mddielkniq lqfpalryls rddnsvreer vetdkgsllv
       61 avqgnrakpa iltyhdlgln yissfqaffn yidmrvllen fcvyhvnapg qeegaptlpe
      121 dyvypsmdel aeqllfvlgh fglksvigfg vgaganilar falahpekvn alclincvst
      181 qagwiewgyq klnvrhlrsq gmtqgvldyl mwhhfgrgte ernhdlvqvy knyferrvnp
      241 tnlalfidsy vrrtdlnitr eldptrkkeg ltlgvpvmni tgalsphvdd tvtlngrldp
      301 ansswmkisd cgmvleeqpg kvseafrlfl qgegyvapls plkmadyrla sleglnhvcs
      361 kkidwptati hitenpisea vvc
//
LOCUS       XP_034195994             474 aa            linear   INV 03-SEP-2025
DEFINITION  misexpression suppressor of KSR 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195994
VERSION     XP_034195994.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340103.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..474
                     /product="misexpression suppressor of KSR 2 isoform X4"
                     /calculated_mol_wt=52749
     Region          124..411
                     /region_name="Ndr"
                     /note="Ndr family; pfam03096"
                     /db_xref="CDD:397285"
     CDS             1..474
                     /gene="MESK2"
                     /coded_by="XM_034340103.2:568..1992"
                     /db_xref="GeneID:117611798"
ORIGIN      
        1 mpagigasyr slgdlgenvy ksttivdqtq ttgqsvlesv kkafsfaspk velrkkdpli
       61 edrresvsiv srernrmpta ataeesallg tmpsdsmddi elkniqlqfp alrylsrddn
      121 svreervetd kgsllvavqg nrakpailty hdlglnyiss fqaffnyidm rvllenfcvy
      181 hvnapgqeeg aptlpedyvy psmdelaeql lfvlghfglk svigfgvgag anilarfala
      241 hpekvnalcl incvstqagw iewgyqklnv rhlrsqgmtq gvldylmwhh fgrgteernh
      301 dlvqvyknyf errvnptnla lfidsyvrrt dlnitreldp trkkegltlg vpvmnitgal
      361 sphvddtvtl ngrldpanss wmkisdcgmv leeqpgkvse afrlflqgeg yvvrsprkpv
      421 tpttpevapl splkmadyrl asleglnhvc skkidwptat ihitenpise avvc
//
LOCUS       XP_034195995             310 aa            linear   INV 03-SEP-2025
DEFINITION  peptidyl-prolyl cis-trans isomerase Fkb14 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195995
VERSION     XP_034195995.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340104.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..310
                     /product="peptidyl-prolyl cis-trans isomerase Fkb14
                     isoform X1"
                     /calculated_mol_wt=34519
     Region          126..218
                     /region_name="FKBP_C"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase;
                     pfam00254"
                     /db_xref="CDD:459735"
     Region          229..302
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     CDS             1..310
                     /gene="Fkbp14"
                     /coded_by="XM_034340104.2:279..1211"
                     /db_xref="GeneID:117611800"
ORIGIN      
        1 mmkkeaeaet yifrganeta qcggtvflvl fqrvypimer staqpsrgeg segswseqsg
       61 rptqlltsir qrtyernmav rflnnagvvl atctvflvfa nilvhadaeq tqstfnvekl
      121 yvpevcdaks kigdqltmhy tgtlqdgtkf dssldrdqpf tfqlgvgqvi kgwdqglvdm
      181 cvgekrklti ppelgygekg agnvipggat llfdvelini sdspptanvf keidsnhdnq
      241 lsreevrtmv seylrkqlie aeqggsenee vkkmlgehdk lveeifqhed kdkngfishd
      301 eftgpkhdel
//
LOCUS       XP_034195996             306 aa            linear   INV 03-SEP-2025
DEFINITION  peptidyl-prolyl cis-trans isomerase Fkb14 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195996
VERSION     XP_034195996.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340105.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..306
                     /product="peptidyl-prolyl cis-trans isomerase Fkb14
                     isoform X2"
                     /calculated_mol_wt=34031
     Region          126..218
                     /region_name="FKBP_C"
                     /note="FKBP-type peptidyl-prolyl cis-trans isomerase;
                     pfam00254"
                     /db_xref="CDD:459735"
     Region          229..298
                     /region_name="EF-hand_7"
                     /note="EF-hand domain pair; pfam13499"
                     /db_xref="CDD:463900"
     CDS             1..306
                     /gene="Fkbp14"
                     /coded_by="XM_034340105.2:16..936"
                     /db_xref="GeneID:117611800"
ORIGIN      
        1 mmkkeaeaet yifrganeta qcggtvflvl fqrvypimer staqpsrgeg segswseqsg
       61 rptqlltsir qrtyernmav rflnnagvvl atctvflvfa nilvhadaeq tqstfnvekl
      121 yvpevcdaks kigdqltmhy tgtlqdgtkf dssldrdqpf tfqlgvgqvi kgwdqglvdm
      181 cvgekrklti ppelgygekg agnvipggat llfdvelini sdspptanvf keidsnhdnq
      241 lsreevseyl rkqlieaeqg gseneevkkm lgehdklvee ifqhedkdkn gfishdeftg
      301 pkhdel
//
LOCUS       XP_034195821            1006 aa            linear   INV 03-SEP-2025
DEFINITION  smg5 nonsense mediated mRNA decay factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195821
VERSION     XP_034195821.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339930.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195821.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1006
                     /product="smg5 nonsense mediated mRNA decay factor isoform
                     X1"
                     /calculated_mol_wt=114224
     Region          77..194
                     /region_name="EST1"
                     /note="Telomerase activating protein Est1; pfam10374"
                     /db_xref="CDD:463062"
     Region          202..>404
                     /region_name="EST1_DNA_bind"
                     /note="Est1 DNA/RNA binding domain; pfam10373"
                     /db_xref="CDD:431239"
     Region          <407..>621
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          840..999
                     /region_name="PIN_Smg5-like"
                     /note="VapC-like PIN domain of human nonsense-mediated
                     decay factor Smg5, and other similar eukaryotic homologs;
                     cd09884"
                     /db_xref="CDD:350232"
     Site            845
                     /site_type="other"
                     /note="conserved Asp residue"
                     /db_xref="CDD:350232"
     CDS             1..1006
                     /gene="Smg5"
                     /coded_by="XM_034339930.2:60..3080"
                     /db_xref="GeneID:117611742"
ORIGIN      
        1 mrrtyntaad vrttdcleqt rrlhkgvtdi akrldeqksr altitdlftp sgetlrgklk
       61 dycvrliikd pvgyarktee llwrkafydi vyvskklrkg ntwteaekam lcvhlavgig
      121 fyhhlilkvq leynldlvgt idfafmqnet rsnaksktsq skhhtedvkq cvmrlihrsl
      181 vclgdlaryk leldpywdsm ianryykmai aidpnigmph nqlgtvagnk nygldavyhy
      241 mrcvlctepf egaegnlkra ivthsictde kcpthmcvsr lfsllqlwdy dvpnsdrinq
      301 ecqdlltnie tclsveqsea gntkynekec nievylqnck ngqtnyltdd mifkivaicl
      361 msvsrlkake ssevqgvvai tlailsqlmq ftitrlqesf idlplpniel dinlkdnern
      421 sesinveeni nlkqsnsnen nnkstiindn ktcekgsaed ngdvhngtrk irdkpksllt
      481 klrrrkrkns sesdasdvdh tissdeinsd iseteddils eenvlsddal sedvtddegt
      541 cverneetae nfetkklngh ierkteseis dtindriqng eeipddqvdk kvndkdsvtn
      601 sgstvtissn nileengess drimyvaqlk kqnlkpdevl kiligkeila sikiccdwlr
      661 snpdiiricg kssrtllkrv tillnlinin adallknckd dsmilssvdk lmecvkvipl
      721 penidlrgln lleeshksln wkmlhkhkin kreetvlrvl kliefghfls svedagvkye
      781 qtkqlfvtid lnasnsakgs gkdwemdhsr gklmrhmgkl wlkaevsale trlrsrlmsp
      841 ylvpdheals khtpalkrlv yakkfivvip svvvsaldev krvsgrarea trwletqlkr
      901 gsrflraqrp herlalpfik gprpkdkeaw lffqiiecch yltqqtkvgm tsdaetpvvt
      961 lltgcnpddk kvltfspegl aksagvniey iesfhtkwka sskshg
//
LOCUS       XP_034195822             982 aa            linear   INV 03-SEP-2025
DEFINITION  smg5 nonsense mediated mRNA decay factor isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195822
VERSION     XP_034195822.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339931.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195822.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..982
                     /product="smg5 nonsense mediated mRNA decay factor isoform
                     X2"
                     /calculated_mol_wt=111337
     Region          53..170
                     /region_name="EST1"
                     /note="Telomerase activating protein Est1; pfam10374"
                     /db_xref="CDD:463062"
     Region          178..>380
                     /region_name="EST1_DNA_bind"
                     /note="Est1 DNA/RNA binding domain; pfam10373"
                     /db_xref="CDD:431239"
     Region          <383..>597
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          816..975
                     /region_name="PIN_Smg5-like"
                     /note="VapC-like PIN domain of human nonsense-mediated
                     decay factor Smg5, and other similar eukaryotic homologs;
                     cd09884"
                     /db_xref="CDD:350232"
     Site            821
                     /site_type="other"
                     /note="conserved Asp residue"
                     /db_xref="CDD:350232"
     CDS             1..982
                     /gene="Smg5"
                     /coded_by="XM_034339931.2:135..3083"
                     /db_xref="GeneID:117611742"
ORIGIN      
        1 mgvtdiakrl deqksralti tdlftpsget lrgklkdycv rliikdpvgy arkteellwr
       61 kafydivyvs kklrkgntwt eaekamlcvh lavgigfyhh lilkvqleyn ldlvgtidfa
      121 fmqnetrsna ksktsqskhh tedvkqcvmr lihrslvclg dlarykleld pywdsmianr
      181 yykmaiaidp nigmphnqlg tvagnknygl davyhymrcv lctepfegae gnlkraivth
      241 sictdekcpt hmcvsrlfsl lqlwdydvpn sdrinqecqd lltnietcls veqseagntk
      301 ynekecniev ylqnckngqt nyltddmifk ivaiclmsvs rlkakessev qgvvaitlai
      361 lsqlmqftit rlqesfidlp lpnieldinl kdnernsesi nveeninlkq snsnennnks
      421 tiindnktce kgsaedngdv hngtrkirdk pkslltklrr rkrknssesd asdvdhtiss
      481 deinsdiset eddilseenv lsddalsedv tddegtcver neetaenfet kklnghierk
      541 teseisdtin driqngeeip ddqvdkkvnd kdsvtnsgst vtissnnile engessdrim
      601 yvaqlkkqnl kpdevlkili gkeilasiki ccdwlrsnpd iiricgkssr tllkrvtill
      661 nlininadal lknckddsmi lssvdklmec vkviplpeni dlrglnllee shkslnwkml
      721 hkhkinkree tvlrvlklie fghflssved agvkyeqtkq lfvtidlnas nsakgsgkdw
      781 emdhsrgklm rhmgklwlka evsaletrlr srlmspylvp dhealskhtp alkrlvyakk
      841 fivvipsvvv saldevkrvs grareatrwl etqlkrgsrf lraqrpherl alpfikgprp
      901 kdkeawlffq iiecchyltq qtkvgmtsda etpvvtlltg cnpddkkvlt fspeglaksa
      961 gvnieyiesf htkwkassks hg
//
LOCUS       XP_034195824             998 aa            linear   INV 03-SEP-2025
DEFINITION  lon protease homolog, mitochondrial [Osmia lignaria lignaria].
ACCESSION   XP_034195824
VERSION     XP_034195824.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339933.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195824.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..998
                     /product="lon protease homolog, mitochondrial"
                     /calculated_mol_wt=112793
     Region          113..968
                     /region_name="lon"
                     /note="endopeptidase La; TIGR00763"
                     /db_xref="CDD:273258"
     CDS             1..998
                     /gene="Lon"
                     /coded_by="XM_034339933.2:277..3273"
                     /db_xref="GeneID:117611743"
ORIGIN      
        1 mrlatamnsk llpvfrhrmt nirsysrnhr vdhdelsvyl rpqfrrikii eshgmfarni
       61 hsrrqnaavi pvrffatkrs dkdatngnne dylsdppslp atvvvpevwp hvpviainrn
      121 pvfprfikli eltnpilidl irrkvklnqp yvgiflkkte eneaevvqnl devysvgtfa
      181 qihevqdlgn rlrlvvmahr rikivgqile dlekpasdel vtsakpsrrs llrkkmeqkv
      241 vepervqkve aeiitpenkn tvpenkeavt eksgekvgpv etppqansns pqpllmvevv
      301 nithekfrqt eeikaltqel iktirdiism nplyreslqq mlhqgqrvvd npvylsdlga
      361 altgadaqel qqvleemdil krlrlslall kkeyelsklq qkigreveek vkqqhrkyil
      421 heqlkvikke lglekddkda iaekyrerir ektvpkvvmd vleeelnkln fleshssefn
      481 vtrnyldwlt smpwgvtsae nlnlqqaiei ldkdhygmed ikkrilefia vsqlkgstqg
      541 kilcfhgppg vgktsiaksi sralnreyfr fsvggmtdva eikghrrtyv gampgkviqc
      601 lkktktenpl ilidevdkig kghqgdpasa llemldpeqn anfldhyldv pvdlskvlfi
      661 ctanvidtip eplrdrmemi dmsgyvaeek laiakqylvp qamndsgltk dqieigdnal
      721 hlliksycre sgvrslqkhi ekvhrkvafk vvkkesekin vtaenlhefv gkpvfthdrm
      781 ydstppgvvm glawtamggs tlfiettirk plisgksegs fevtghlgdv mkesiqialt
      841 vsrnflklke psntflydsh lhlhvpegat pkdgpsagit iaialislak nkairqnvam
      901 tgelslmgrv lpvggikeki iaakrvgvnc vilpeenkkd fndlakyitd glevhfattf
      961 edvyricfet eqadqlqypk lnvnistaqs apllskkd
//
LOCUS       XP_034195825             890 aa            linear   INV 03-SEP-2025
DEFINITION  transient receptor potential cation channel family member painless
            [Osmia lignaria lignaria].
ACCESSION   XP_034195825
VERSION     XP_034195825.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339934.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195825.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..890
                     /product="transient receptor potential cation channel
                     family member painless"
                     /calculated_mol_wt=100970
     Region          <5..241
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          67..98
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          105..133
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(107..108,111..113,115..116,120,123,133,134,136,
                     140..141,144..146,148..149,153,156,165,167,169,173..174,
                     177..179,181..182,186,192)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          134..165
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          167..198
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          297..398
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          367..391
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          498..721
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     CDS             1..890
                     /gene="pain"
                     /coded_by="XM_034339934.2:149..2821"
                     /db_xref="GeneID:117611745"
ORIGIN      
        1 mdpedesvqt hllhnytavs trsqiiykll ldslrskdig sfknlveqsl kkqpfgidin
       61 ypypsrsdet cldiackngl pefvrfllek gakvntinea hnrgpihfat enghvqvlgv
      121 lldeptlnpn leagqqtalh mavkkndlnc aelliekgas pnipnnkglt alhmaamkgq
      181 kdmvnlilqk skhgldldny kdynnqttrd vlqknftdip lpavqkrgvn vhdlnyylna
      241 ndeanflksl klvqddvvnn vaedliemav qrnfkdtvat llertkgngc sleksaiiai
      301 qqglpnilrq ilnmdteidk nllllnacve lgipgnegse smsnrleclk iilerdddvd
      361 vrcadskgnt plhyaaranc reavtlllek gsyigqmnnf gvppvedisv smlsqyfddc
      421 iqarkertne ytiefdykcl mpheytsyqe knsmnqgkre mdifryiadn sglkhllkhp
      481 llssflylkw hkiryllyln fafyvlfyls lnayilhvty ikdpkmvtns sdldgsirst
      541 svnvlqiltg illiflalrk ilqlisspch yvssfeywle mtliflgfat lcdagtqfav
      601 iaillsawem iiligqhprm stgiemfktv slnfmrflfl yaflifafal afftlfkngd
      661 ddnfpdpghs lfktiimltg eldandipfl lhpvlshlvf vlfvfliaiv lfnllnglav
      721 sdtadilgka elvglisrvr vlayienvav tvpfihgphc cicygilhgw kwnpfaflre
      781 kvllfpkylk sgklsvkpyd sleafnnrry ykvrsnensk gkvfptfkmd phiikqakei
      841 lskrgqesds drvirqlemm kerlalmeit lntikqgien nnfnvaredd
//
LOCUS       XP_034195826             844 aa            linear   INV 03-SEP-2025
DEFINITION  gamma-tubulin complex component 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195826
VERSION     XP_034195826.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339935.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195826.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="gamma-tubulin complex component 2 isoform X1"
                     /calculated_mol_wt=97145
     Region          195..483
                     /region_name="GCP_N_terminal"
                     /note="Gamma tubulin complex component N-terminal;
                     pfam17681"
                     /db_xref="CDD:465456"
     Region          486..825
                     /region_name="GCP_C_terminal"
                     /note="Gamma tubulin complex component C-terminal;
                     pfam04130"
                     /db_xref="CDD:461187"
     CDS             1..844
                     /gene="LOC117611746"
                     /coded_by="XM_034339935.2:176..2710"
                     /db_xref="GeneID:117611746"
ORIGIN      
        1 msefklhhlv veligllgss sapekhverl qkegiptsas altvvasqss irnlarnspv
       61 pelflqkyee lkakkvdllg lfiqvlelis qdkelksyla kqastltsss tknsaitted
      121 lpqicknvik aavegekkln kqvntvakks epsvikhnwv herpritwdf ynepnvtpcq
      181 kvvpvvsqes illwdilycl kgidgsyivs esltglyavk tfnispdvgi sykqmtqqil
      241 plashysmta rfveekvlpe dgqvnhalrg aikcllkdyl lfvvqlemeh vrgklnlqkl
      301 wfyiqptmia msilfqitst ickanakggk vlsllheqan nisgeakfke lclfliqtas
      361 vpymqilekw vykgvicdpy eeffvednel ihreelpidy sadywekryt mrperiptfl
      421 nehaqtilrt gkyfnvirqc gktvqwgkqe pliyqhqgqk yiaaidrays eaaktllevl
      481 ihendlmgrl rsvksyflla qgdfvvqfmn lceaelnksm ydivihrlas llevalrmst
      541 adsdpykddl kpellpydlq fqmfrilsiq trdekeycfq tdktltglea fvfnydvkwp
      601 vslilnrkai acyqmlfrhl fyckyierrl crvwvsnkia ktfthnvams yrqafslrqr
      661 mldciqhlay ymmveviepn wltflnkmsk vsnvddvlsv hqdlqdsylk ecmltdpdll
      721 gcitgicaac ldfcnfmerm spyyidaelt smigayqddv ydsedeegdt skenaasfee
      781 mivlldekft qvlirlldri cdlgcdnnne kllnvfcrld fnlfytdili qrgrekttqe
      841 dvsg
//
LOCUS       XP_034195827             818 aa            linear   INV 03-SEP-2025
DEFINITION  gamma-tubulin complex component 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195827
VERSION     XP_034195827.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339936.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195827.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..818
                     /product="gamma-tubulin complex component 2 isoform X2"
                     /calculated_mol_wt=94130
     Region          195..483
                     /region_name="GCP_N_terminal"
                     /note="Gamma tubulin complex component N-terminal;
                     pfam17681"
                     /db_xref="CDD:465456"
     Region          486..799
                     /region_name="GCP_C_terminal"
                     /note="Gamma tubulin complex component C-terminal;
                     pfam04130"
                     /db_xref="CDD:461187"
     CDS             1..818
                     /gene="LOC117611746"
                     /coded_by="XM_034339936.2:174..2630"
                     /db_xref="GeneID:117611746"
ORIGIN      
        1 msefklhhlv veligllgss sapekhverl qkegiptsas altvvasqss irnlarnspv
       61 pelflqkyee lkakkvdllg lfiqvlelis qdkelksyla kqastltsss tknsaitted
      121 lpqicknvik aavegekkln kqvntvakks epsvikhnwv herpritwdf ynepnvtpcq
      181 kvvpvvsqes illwdilycl kgidgsyivs esltglyavk tfnispdvgi sykqmtqqil
      241 plashysmta rfveekvlpe dgqvnhalrg aikcllkdyl lfvvqlemeh vrgklnlqkl
      301 wfyiqptmia msilfqitst ickanakggk vlsllheqan nisgeakfke lclfliqtas
      361 vpymqilekw vykgvicdpy eeffvednel ihreelpidy sadywekryt mrperiptfl
      421 nehaqtilrt gkyfnvirqc gktvqwgkqe pliyqhqgqk yiaaidrays eaaktllevl
      481 ihendlmgrl rsvksyflla qgdfvvqfmn lceaelnksm ydivihrlas llevalrmst
      541 adsdpykddl kpellpydlq fqmfrilsiq trdekeycfq tdktltglea fvfnydvkwp
      601 vslilnrkai acyqmlfrhl fyckyierrl crvwvsnkia ktfthnvams yrqafslrqr
      661 mldciqhlay ymmveviepn wltflnkmsk vsnvddvlsv hqdlqdsylk ecmltdpdll
      721 gcitgicaac ldfcnfmede egdtskenaa sfeemivlld ekftqvlirl ldricdlgcd
      781 nnnekllnvf crldfnlfyt diliqrgrek ttqedvsg
//
LOCUS       XP_034195830             650 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611747 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195830
VERSION     XP_034195830.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339939.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195830.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..650
                     /product="uncharacterized protein LOC117611747 isoform X1"
                     /calculated_mol_wt=70161
     CDS             1..650
                     /gene="LOC117611747"
                     /coded_by="XM_034339939.2:185..2137"
                     /db_xref="GeneID:117611747"
ORIGIN      
        1 mggmtqrpwt ptkrrgpiaa eysspgpacv tlppligkti pdskrgrapa ysfgsrhssk
       61 ndspgpgpgq ynvsglsakg kdgapasslh grtkstkpei tpgpcdynpe kaekvildss
      121 pkysfgiktq tekidctpap sdyctekvnl dntpkysfgl kpvlekpsdi papgtyypek
      181 vrldhapqft fglrpsiekp sdtpapgtys pekvnldkgp qysltgkgre gkpddtpapg
      241 tycpekvkld ntpqftfgsr tplerindtp apgtyspekv hldkgpqfsl sgktfydksd
      301 nnpapgtysp ekvnldkspq ysfglrvpld kpsnnpgpge ycpekvnlnk gpeyslsgrr
      361 pseklsdtpa pgtyspekvn lnkgpqysly gkgmsikpdd npapgtyspe kinlnkspqf
      421 sisgkgivek pndnpapgty spekvnldks pqysfglktf vekpndtpap gsyhpekvnl
      481 nkspqysfgi ktevhgidsv pgpgtyspek amlllekalq ftfglrtnvs rpndipapnl
      541 ynipsalggt kegnkkaapa ysisgrqkvf tddrvlvpgp gtyeavkpda irvkspaysm
      601 saryqlpddh sqipgpgayc pekvildipp ahsfgirhsp ymcnlkdvvy
//
LOCUS       XP_076546760             617 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611747 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546760
VERSION     XP_076546760.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690645.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..617
                     /product="uncharacterized protein LOC117611747 isoform X2"
                     /calculated_mol_wt=66512
     CDS             1..617
                     /gene="LOC117611747"
                     /coded_by="XM_076690645.1:184..2037"
                     /db_xref="GeneID:117611747"
ORIGIN      
        1 mggmtqrpwt ptkrrgpiaa eysspgpacv tlppligkti pdskrgrapa ysfgsrhssk
       61 ndspgpgpgq ynvsglsakg kdgapasslh grtkstkpei tpgpcdynpe kaekvildss
      121 pkysfgiktq tekidctpap gtyypekvrl dhapqftfgl rpsiekpsdt papgtyspek
      181 vnldkgpqys ltgkgregkp ddtpapgtyc pekvkldntp qftfgsrtpl erindtpapg
      241 tyspekvhld kgpqfslsgk tfydksdnnp apgtyspekv nldkspqysf glrvpldkps
      301 nnpgpgeycp ekvnlnkgpe yslsgrrpse klsdtpapgt yspekvnlnk gpqyslygkg
      361 msikpddnpa pgtyspekin lnkspqfsis gkgivekpnd npapgtyspe kvnldkspqy
      421 sfglktfvek pndtpapgsy hpekvnlnks pqysfgikte vhgidsvpgp gtyspekaml
      481 llekalqftf glrtnvsrpn dipapnlyni psalggtkeg nkkaapaysi sgrqkvftdd
      541 rvlvpgpgty eavkpdairv kspaysmsar yqlpddhsqi pgpgaycpek vildippahs
      601 fgirhspymc nlkdvvy
//
LOCUS       XP_076546761             617 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611747 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546761
VERSION     XP_076546761.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690646.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..617
                     /product="uncharacterized protein LOC117611747 isoform X3"
                     /calculated_mol_wt=66651
     CDS             1..617
                     /gene="LOC117611747"
                     /coded_by="XM_076690646.1:185..2038"
                     /db_xref="GeneID:117611747"
ORIGIN      
        1 mggmtqrpwt ptkrrgpiaa eysspgpacv tlppligkti pdskrgrapa ysfgsrhssk
       61 ndspgpgpgq ynvsglsakg kdgapasslh grtkstkpei tpgpcdynpe kaekvildss
      121 pkysfgiktq tekidctpap sdyctekvnl dntpkysfgl kpvlekpsdi papgtyypek
      181 vrldhapqft fglrpsiekp sdtpapgtys pekvnldkgp qysltgkgre gkpddtpapg
      241 tycpekvkld ntpqftfgsr tplerindtp apgtyspekv hldkgpqfsl sgktfydksd
      301 nnpapgtysp ekvnldkspq ysfglrvpld kpsnnpgpge ycpekvnlnk gpeyslsgrr
      361 pseklsdtpa pgtyspekvn lnkgpqysly gkgmsikpdd npapgtyspe kvnldkspqy
      421 sfglktfvek pndtpapgsy hpekvnlnks pqysfgikte vhgidsvpgp gtyspekaml
      481 llekalqftf glrtnvsrpn dipapnlyni psalggtkeg nkkaapaysi sgrqkvftdd
      541 rvlvpgpgty eavkpdairv kspaysmsar yqlpddhsqi pgpgaycpek vildippahs
      601 fgirhspymc nlkdvvy
//
LOCUS       XP_076546762             641 aa            linear   INV 03-SEP-2025
DEFINITION  polypeptide N-acetylgalactosaminyltransferase 35A [Osmia lignaria
            lignaria].
ACCESSION   XP_076546762
VERSION     XP_076546762.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690647.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="polypeptide N-acetylgalactosaminyltransferase
                     35A"
                     /calculated_mol_wt=74192
     Region          175..470
                     /region_name="pp-GalNAc-T"
                     /note="pp-GalNAc-T initiates the formation of mucin-type
                     O-linked glycans; cd02510"
                     /db_xref="CDD:133004"
     Site            order(179..181,183,212,243,246,250,266..268,348..349,
                     371..372,374,399,402,407)
                     /site_type="active"
                     /note="ligand binding site [active]"
                     /db_xref="CDD:133004"
     Site            order(266,268,399)
                     /site_type="active"
                     /note="Mn binding site [active]"
                     /db_xref="CDD:133004"
     Region          504..630
                     /region_name="beta-trefoil_Ricin_GALNT11"
                     /note="ricin B-type lectin domain, beta-trefoil fold,
                     found in polypeptide N-acetylgalactosaminyltransferase 11
                     (GALNT11) and similar proteins; cd23440"
                     /db_xref="CDD:467318"
     Site            order(521..524,534,536,539,543..544)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467318"
     Site            order(563..566,570,572,575,580..581)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467318"
     Site            order(603..606,614,616,619,623..624)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467318"
     CDS             1..641
                     /gene="Pgant35A"
                     /coded_by="XM_076690647.1:185..2110"
                     /db_xref="GeneID:117611748"
ORIGIN      
        1 mmstryvsfi sgmviasltw afslylysrl sqntdavnpt mlisentkll deftydrkky
       61 naksqnnlmv hrdkeswmdk gvynlkgnnf knsdkllqql qpvpiksavt lagqgldelg
      121 mirnfddqrk reegyknysf nilvsdnigl hrelpdtrhk lcelqkysnk lpnasivicf
      181 ynehyttllr slhsiierts khllheiilv ndwsdskelh gkmkvyidnn fdgkvkffkt
      241 ekreglirar mfgarkatge vlifldshie vnkmwvepll sriarsktvv ampvidiinp
      301 dtfqytdspl vrggfnwglh fkwdnlpvgt lvhdedfikp iksptmaggl famdreyfik
      361 lgeydagmdv wggenleisf riwmcggnie lipcsrvghv frkrrpygtd dqhdtmlkns
      421 lrvayvwlde ykdyflrnvk ridygditdr lnlreklkck nfswylkvvy pelalpddnk
      481 srlkdkwakv eqkamqpwhs rkrnytdqyq irlsntalci qsekdiktkg aklilmpclr
      541 iksqmwyetd kkelvlgqml cmeaaekyak lgkchemggn qewrnksakg tpvynmaagt
      601 clgasqaakg aqvimdlctk sdtsfiswdl vrskilskei r
//
LOCUS       XP_034195831             641 aa            linear   INV 03-SEP-2025
DEFINITION  polypeptide N-acetylgalactosaminyltransferase 35A [Osmia lignaria
            lignaria].
ACCESSION   XP_034195831
VERSION     XP_034195831.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339940.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195831.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..641
                     /product="polypeptide N-acetylgalactosaminyltransferase
                     35A"
                     /calculated_mol_wt=74192
     Region          175..470
                     /region_name="pp-GalNAc-T"
                     /note="pp-GalNAc-T initiates the formation of mucin-type
                     O-linked glycans; cd02510"
                     /db_xref="CDD:133004"
     Site            order(179..181,183,212,243,246,250,266..268,348..349,
                     371..372,374,399,402,407)
                     /site_type="active"
                     /note="ligand binding site [active]"
                     /db_xref="CDD:133004"
     Site            order(266,268,399)
                     /site_type="active"
                     /note="Mn binding site [active]"
                     /db_xref="CDD:133004"
     Region          504..630
                     /region_name="beta-trefoil_Ricin_GALNT11"
                     /note="ricin B-type lectin domain, beta-trefoil fold,
                     found in polypeptide N-acetylgalactosaminyltransferase 11
                     (GALNT11) and similar proteins; cd23440"
                     /db_xref="CDD:467318"
     Site            order(521..524,534,536,539,543..544)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467318"
     Site            order(563..566,570,572,575,580..581)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467318"
     Site            order(603..606,614,616,619,623..624)
                     /site_type="other"
                     /note="putative sugar binding site [chemical binding]"
                     /db_xref="CDD:467318"
     CDS             1..641
                     /gene="Pgant35A"
                     /coded_by="XM_034339940.2:104..2029"
                     /db_xref="GeneID:117611748"
ORIGIN      
        1 mmstryvsfi sgmviasltw afslylysrl sqntdavnpt mlisentkll deftydrkky
       61 naksqnnlmv hrdkeswmdk gvynlkgnnf knsdkllqql qpvpiksavt lagqgldelg
      121 mirnfddqrk reegyknysf nilvsdnigl hrelpdtrhk lcelqkysnk lpnasivicf
      181 ynehyttllr slhsiierts khllheiilv ndwsdskelh gkmkvyidnn fdgkvkffkt
      241 ekreglirar mfgarkatge vlifldshie vnkmwvepll sriarsktvv ampvidiinp
      301 dtfqytdspl vrggfnwglh fkwdnlpvgt lvhdedfikp iksptmaggl famdreyfik
      361 lgeydagmdv wggenleisf riwmcggnie lipcsrvghv frkrrpygtd dqhdtmlkns
      421 lrvayvwlde ykdyflrnvk ridygditdr lnlreklkck nfswylkvvy pelalpddnk
      481 srlkdkwakv eqkamqpwhs rkrnytdqyq irlsntalci qsekdiktkg aklilmpclr
      541 iksqmwyetd kkelvlgqml cmeaaekyak lgkchemggn qewrnksakg tpvynmaagt
      601 clgasqaakg aqvimdlctk sdtsfiswdl vrskilskei r
//
LOCUS       XP_076546763             615 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611749 [Osmia lignaria lignaria].
ACCESSION   XP_076546763
VERSION     XP_076546763.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690648.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..615
                     /product="uncharacterized protein LOC117611749"
                     /calculated_mol_wt=67297
     Region          <275..407
                     /region_name="Jiraiya"
                     /note="pfam15038"
                     /db_xref="CDD:464461"
     CDS             1..615
                     /gene="LOC117611749"
                     /coded_by="XM_076690648.1:372..2219"
                     /db_xref="GeneID:117611749"
ORIGIN      
        1 mlkhiltgqp ssagsrhhhn eyqassgslh gghhhhhhhh qqdqqhqhhh ynqssnvrps
       61 gnggrsvdly dsvvhpssqq ranvhqaatt pssthrhpln hsqlsvnnls qrlnhshaln
      121 lstlstskhs vnsvspvagg nnnnnnnnnn nnnhlsnalg htvvpaapvh qdnrpkvngg
      181 fdisrlsrlp sqttpspapv lqatvlggql tggggsialp lnaswsssls rnhkdheaga
      241 ketltslgll clvslllall slifllkisp ltvtpsslis peeytivyev tlalcalals
      301 lnlccllvca iqflftvklv ktsyqghwsn kylqkssisr icavggffis ipvfltgmil
      361 ytfiqfhstp aivtsvfigl givfcgcamv hnvfvwqrek tnavkalare qceaavqlqr
      421 qqqlqhhqnq pqlhhqqhqh hqqqqqqqqq qqqqqqqqqq qqqqqqqqqq qqqqqqqlrt
      481 pltmhhpgca tkvgslasqp nashtthgra aqyqhyhhhh hhrhvssmsp pllisspapm
      541 tashnhlnvs shrnvitppd tpgdrsppsp gnklnrsqhl preasgsvsp glpaatldls
      601 saattnsphe lstlv
//
LOCUS       XP_034195844             403 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylglucosamine-6-phosphate deacetylase isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195844
VERSION     XP_034195844.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339953.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195844.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="N-acetylglucosamine-6-phosphate deacetylase
                     isoform X1"
                     /calculated_mol_wt=44114
     Region          6..390
                     /region_name="NagA"
                     /note="N-acetylglucosamine-6-phosphate deacetylase, NagA,
                     catalyzes the hydrolysis of the N-acetyl group of
                     N-acetyl-glucosamine-6-phosphate (GlcNAc-6-P) to
                     glucosamine 6-phosphate and acetate. This is the first
                     committed step in the biosynthetic pathway to...; cd00854"
                     /db_xref="CDD:238434"
     Site            order(64,66,136,147,203,224,227..228,264,286,320)
                     /site_type="active"
                     /db_xref="CDD:238434"
     Site            order(226,232,234..235,237,244..245,263..266,268..269,
                     271..272,275)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238434"
     CDS             1..403
                     /gene="LOC117611751"
                     /coded_by="XM_034339953.2:80..1291"
                     /db_xref="GeneID:117611751"
ORIGIN      
        1 msqilkqfyn ccilrggkil nedlwvrdgk ivnpekifyd ekikpnirvd cngalispgy
       61 idlqinggfg idfthnvdnv qeginkvakk liefgvtsfc ptlvtspset yykilpnirk
      121 rsggihgasi lgihlegpfi spskkgahpe nyieqfdkgf qsvikmygtl envclvtlap
      181 elpnsktvik elckrnikvs lghsianlhe geeavksgas fithlfnaml pfhhrdpglv
      241 glltsdkips erivhygiia dgihthpaal riahrthpeg lvlvtdalsa lgleegvhql
      301 gqlkiemrsg cayiagtdtl cgstaemskc vrhfkeatgc svvealeaat lhpaktlgie
      361 ktkgildyea dadfvmldkd lkllstwisg ecvystynyv esk
//
LOCUS       XP_076546764             411 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylglucosamine-6-phosphate deacetylase isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546764
VERSION     XP_076546764.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690649.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..411
                     /product="N-acetylglucosamine-6-phosphate deacetylase
                     isoform X2"
                     /calculated_mol_wt=44973
     Region          6..398
                     /region_name="NagA"
                     /note="N-acetylglucosamine-6-phosphate deacetylase, NagA,
                     catalyzes the hydrolysis of the N-acetyl group of
                     N-acetyl-glucosamine-6-phosphate (GlcNAc-6-P) to
                     glucosamine 6-phosphate and acetate. This is the first
                     committed step in the biosynthetic pathway to...; cd00854"
                     /db_xref="CDD:238434"
     Site            order(64,66,136,147,211,232,235..236,272,294,328)
                     /site_type="active"
                     /db_xref="CDD:238434"
     Site            order(234,240,242..243,245,252..253,271..274,276..277,
                     279..280,283)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238434"
     CDS             1..411
                     /gene="LOC117611751"
                     /coded_by="XM_076690649.1:41..1276"
                     /db_xref="GeneID:117611751"
ORIGIN      
        1 msqilkqfyn ccilrggkil nedlwvrdgk ivnpekifyd ekikpnirvd cngalispgy
       61 idlqinggfg idfthnvdnv qeginkvakk liefgvtsfc ptlvtspset yykilpnirk
      121 rsggihgasi lgihlegpfi spskkgahpe nyieqfdkas llnvfngfqs vikmygtlen
      181 vclvtlapel pnsktvikel ckrnikvslg hsianlhege eavksgasfi thlfnamlpf
      241 hhrdpglvgl ltsdkipser ivhygiiadg ihthpaalri ahrthpeglv lvtdalsalg
      301 leegvhqlgq lkiemrsgca yiagtdtlcg staemskcvr hfkeatgcsv vealeaatlh
      361 paktlgiekt kgildyeada dfvmldkdlk llstwisgec vystynyves k
//
LOCUS       XP_034195845             209 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611752 [Osmia lignaria lignaria].
ACCESSION   XP_034195845
VERSION     XP_034195845.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339954.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195845.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="uncharacterized protein LOC117611752"
                     /calculated_mol_wt=24392
     CDS             1..209
                     /gene="LOC117611752"
                     /coded_by="XM_034339954.2:75..704"
                     /db_xref="GeneID:117611752"
ORIGIN      
        1 mdlksissri ifsdniirqs lnkqpivsvt lddddqffqk iksdidrnlp htkenykpnn
       61 yyqgtslkrk kcifdeetqn iikrfkkern hkihtlqtsr dinipqsnhv dnnmkqihdi
      121 itnintnkni iqetnmfssn vlttnlrnpk nyvfqmaskl qknvlpfptv ettilkeeye
      181 eslffamflt ppdnnsiedn egfetcsti
//
LOCUS       XP_034195846             187 aa            linear   INV 03-SEP-2025
DEFINITION  gonadal protein gdl isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195846
VERSION     XP_034195846.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339955.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195846.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..187
                     /product="gonadal protein gdl isoform X1"
                     /calculated_mol_wt=21553
     Region          22..184
                     /region_name="DGCR6"
                     /note="DiGeorge syndrome critical region 6 (DGCR6)
                     protein; cl46389"
                     /db_xref="CDD:480729"
     CDS             1..187
                     /gene="gdl"
                     /coded_by="XM_034339955.2:35..598"
                     /db_xref="GeneID:117611753"
ORIGIN      
        1 medygfvfpd diamdvpapt pqdlqrklyf lveqlqhmag elpakyqmrl pyellsglan
       61 sllndtifei vkglmeiqhv tekhlfqqrl qllnqqkiem qeslssiste eertamktal
      121 ykkhkdelkq ldmklvlqld qkvadqqsil ekagvpgfyv tnnpieiqvq mrlcdfiirl
      181 skmevps
//
LOCUS       XP_034195847             174 aa            linear   INV 03-SEP-2025
DEFINITION  gonadal protein gdl isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195847
VERSION     XP_034195847.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339956.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195847.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..174
                     /product="gonadal protein gdl isoform X2"
                     /calculated_mol_wt=20052
     Region          9..171
                     /region_name="DGCR6"
                     /note="DiGeorge syndrome critical region 6 (DGCR6)
                     protein; cl46389"
                     /db_xref="CDD:480729"
     CDS             1..174
                     /gene="gdl"
                     /coded_by="XM_034339956.2:176..700"
                     /db_xref="GeneID:117611753"
ORIGIN      
        1 mdvpaptpqd lqrklyflve qlqhmagelp akyqmrlpye llsglansll ndtifeivkg
       61 lmeiqhvtek hlfqqrlqll nqqkiemqes lssisteeer tamktalykk hkdelkqldm
      121 klvlqldqkv adqqsileka gvpgfyvtnn pieiqvqmrl cdfiirlskm evps
//
LOCUS       XP_034195848             174 aa            linear   INV 03-SEP-2025
DEFINITION  gonadal protein gdl isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195848
VERSION     XP_034195848.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339957.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195848.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..174
                     /product="gonadal protein gdl isoform X2"
                     /calculated_mol_wt=20052
     Region          9..171
                     /region_name="DGCR6"
                     /note="DiGeorge syndrome critical region 6 (DGCR6)
                     protein; cl46389"
                     /db_xref="CDD:480729"
     CDS             1..174
                     /gene="gdl"
                     /coded_by="XM_034339957.2:149..673"
                     /db_xref="GeneID:117611753"
ORIGIN      
        1 mdvpaptpqd lqrklyflve qlqhmagelp akyqmrlpye llsglansll ndtifeivkg
       61 lmeiqhvtek hlfqqrlqll nqqkiemqes lssisteeer tamktalykk hkdelkqldm
      121 klvlqldqkv adqqsileka gvpgfyvtnn pieiqvqmrl cdfiirlskm evps
//
LOCUS       XP_034195849             174 aa            linear   INV 03-SEP-2025
DEFINITION  gonadal protein gdl isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195849
VERSION     XP_034195849.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339958.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195849.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..174
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..174
                     /product="gonadal protein gdl isoform X2"
                     /calculated_mol_wt=20052
     Region          9..171
                     /region_name="DGCR6"
                     /note="DiGeorge syndrome critical region 6 (DGCR6)
                     protein; cl46389"
                     /db_xref="CDD:480729"
     CDS             1..174
                     /gene="gdl"
                     /coded_by="XM_034339958.2:170..694"
                     /db_xref="GeneID:117611753"
ORIGIN      
        1 mdvpaptpqd lqrklyflve qlqhmagelp akyqmrlpye llsglansll ndtifeivkg
       61 lmeiqhvtek hlfqqrlqll nqqkiemqes lssisteeer tamktalykk hkdelkqldm
      121 klvlqldqkv adqqsileka gvpgfyvtnn pieiqvqmrl cdfiirlskm evps
//
LOCUS       XP_076546766             154 aa            linear   INV 03-SEP-2025
DEFINITION  gonadal protein gdl isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546766
VERSION     XP_076546766.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690651.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..154
                     /product="gonadal protein gdl isoform X3"
                     /calculated_mol_wt=17834
     Region          22..151
                     /region_name="DGCR6"
                     /note="DiGeorge syndrome critical region 6 (DGCR6)
                     protein; cl46389"
                     /db_xref="CDD:480729"
     CDS             1..154
                     /gene="gdl"
                     /coded_by="XM_076690651.1:16..480"
                     /db_xref="GeneID:117611753"
ORIGIN      
        1 medygfvfpd diamdvpapt pqdlqrklyf lveqlqhmag lmeiqhvtek hlfqqrlqll
       61 nqqkiemqes lssisteeer tamktalykk hkdelkqldm klvlqldqkv adqqsileka
      121 gvpgfyvtnn pieiqvqmrl cdfiirlskm evps
//
LOCUS       XP_034195945            4133 aa            linear   INV 03-SEP-2025
DEFINITION  dynein axonemal heavy chain 1 [Osmia lignaria lignaria].
ACCESSION   XP_034195945
VERSION     XP_034195945.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340054.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195945.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4133
                     /product="dynein axonemal heavy chain 1"
                     /calculated_mol_wt=476096
     Region          903..1306
                     /region_name="DHC_N2"
                     /note="Dynein heavy chain, N-terminal region 2; pfam08393"
                     /db_xref="CDD:462462"
     Region          1106..3773
                     /region_name="DYN1"
                     /note="Dynein, heavy chain [Cytoskeleton]; COG5245"
                     /db_xref="CDD:227570"
     Region          1433..1759
                     /region_name="AAA_6"
                     /note="Hydrolytic ATP binding site of dynein motor region;
                     pfam12774"
                     /db_xref="CDD:463697"
     Region          2426..2687
                     /region_name="AAA_8"
                     /note="P-loop containing dynein motor region D4;
                     pfam12780"
                     /db_xref="CDD:463701"
     Region          3833..4129
                     /region_name="Dynein_C"
                     /note="Dynein heavy chain C-terminal domain; pfam18199"
                     /db_xref="CDD:465677"
     CDS             1..4133
                     /gene="LOC117611781"
                     /coded_by="XM_034340054.2:263..12664"
                     /db_xref="GeneID:117611781"
ORIGIN      
        1 menrkikrrg yyseiitgta thpvrpeval lnaapkrvkl lwqvltehhs arvqelkdhe
       61 kynflgeakr witfpqdrsi vfpaetfapn vqkeqnvkpk elprnveier lrrsyrnlki
      121 edvleedgis pkemlppavv lsllsheqiy glfatasilp leifddeeyd crttedwisl
      181 siidgvrypl paevfvqkyr etdevaridd ealnhlykwy daavtyydyk hklwsvltld
      241 glkrkfllpr iyirfyaenp rvfakrlata iehrkiadas ikynfyldcm qmdgmltlne
      301 kqkeaivrlt lngirikfpe trvsalinei sldyqrtmcd lmwrkmiqwn pemfkfiawt
      361 psveeirvpd kgkidtgmen fmkvqkfihw vtlyvqeevy qamacivarc iqlstmnlfg
      421 vnygklitls efeemhsqst vsvvkylkep wlekiaqsvr mclrdigkgw fdlkqkvpgv
      481 ydvmklkrfm nlttlrmqya lrvlvensit lygntlegpa lclldveetf iwgnnlvdsq
      541 fksvynpift vdlqmdgnka yysvdpdqfg etiitlydna ltlchqirqi npfllpalkf
      601 ppdlflssvg llegyvcqir drlkiayqkc smilkayvke ytayldfynl kvaeyiqkfk
      661 deehntaeik eeitfhktmi snldftlpkr isigpfnvnv rplknflmgk reecatgllv
      721 mfterlraev dnileeytqi ryklkevpqs iehifeirdw metiplrvqd lqekmeilkl
      781 dfdildsfyw nlsdedfeak wlvigsplqi eneidetner fveeqdkfyk iqiqdevlll
      841 eridtlvgnv sniglqtdin rvhetaldvk rvwkgmtdcr emglllnerq klfgmkvvpf
      901 ehlhklmref epyrslwvta sdwlrwyevw mdnplinvdg sqieshvmdm yrvitrgvrt
      961 fqefpkvqai attirdqide fkpyigliqa lrnpgmkdrh fdqlsaqtgi qmaltpsmtf
     1021 ktllflgiee feelvktvad taakeyater tlnkmiiewe tivmeilpyk atgtyimkvt
     1081 eetlmlldnh ilnvqqlafs plktvfedei neweeklklt qevlilwiev qrdwmylepi
     1141 ftseditvql pvearrynam ernwrrimkn akdnpyiiki cpdsnllegl qecksllemv
     1201 qkglsdylev krkvfprfyf lsdeelleil aqaknvyavq phlrkcfeni qqvkfesdle
     1261 itrmysaege evifrpsmyp vrsveywlgd lenvmrntir eiirealrmi dsisrktwvy
     1321 mwpgqitlcc gqtywtahve ngilrnnlrg yyeellehld dlrdlvrapq tdvqrlmlea
     1381 vitvevhard vlhrlieekv snvndfdwis qlryywvdds elkvravnae fsygyeylgn
     1441 tgrlvitplt drcyltltga lhlkfggapa gpagtgktet tkdlakafai qcvvfncsdq
     1501 ldfmsmgkff kglasagawa cfdefnridi evlsviaqqi ttiqqaqqmr vetfvfegde
     1561 ivlkpscavf itmnpgyagr telpdnlkal frpvammvpd yaliaeislf sygftdaktl
     1621 agkitttfkl sseqlstqdh ydfgmravkt viavagnlkr eqkdlneqqi clralrdvnv
     1681 pkflkddltl fngivsdlfp lleekhvdyg ileaeirasi rkmgmeeide fvkkviqlye
     1741 ttvvrhglml vgptgsaktk cyevlqqact klkgqpqpsg kpftrvlthv lnpksitmgq
     1801 lygeydlntr ewtdgifstl lragiatddd nkrwyifdgp vdalwienmn tvlddnkklc
     1861 lssgeimkil ptmtmmfeva dlrvaspatv srcgmvylep eglgleplvn cwlkslpknm
     1921 tdhvdgiael tghyllpglq vlrsslheiv stvdsgmvqs yinlmnfrig pmagrdgrpp
     1981 paapfqqlip tllspwaafa avwslgassd ynsrcifsew irqvqkdkqh slpfpkdglv
     2041 fdyrlhdggf tdpieshepv apkwykwldd ipsititpem kfadievptm dsvrsatlvg
     2101 yllinnsnal cigptgsgkt laistklsrn mpkkficdfi tfsarttanq tqdlideklt
     2161 krrkdvygpp llrkqvffid dlnmpaldty gaqppielir qfmdfegwyd rkeigsfrri
     2221 edvnfvgamg ppgggrnpvt arllrhfhfi afpemeddak rhifgtilns wlsktlfanm
     2281 ldiflgttln lyttickell ptphkshytf nlrdlskvfq gmlmmnpqki emrenllrlw
     2341 yhenvrvfsd rlindtdrtw fddllretmr kkfeidpqmv vgetmlfysd fcaankeyeq
     2401 itdmkkmdhm lnefledyna tttspmklvl fqdaidhicr inrilrqprg nalllgmggs
     2461 grqsltrlas hiqdyncfqi elsgaytthd wrddikhsmm kagvqaqlmv flfsdtqikd
     2521 dsmledlnsv lnngdvpniy qmdemeriyh smrgqvqeag lqinrsnlfs ayvktvrnnl
     2581 hvvitmspig evfrarirqf palvncctid wfcpwpaaal qsvamrflad ikdesinadi
     2641 lrsivklcey mqssvieasd lylkelnrhn yvtptsylel lsnygnllgk kktelksaeh
     2701 rlsagldkla saevevkkmq ellasmkpkl eraaeatakm ierithdtie aektragake
     2761 qeaiagklki qnqmirdeae adlsearpml iaaekslkal nrnditevka mkrppvgvll
     2821 vietvciinn vkpvkvdtgt ygkgavkldy wgpgsqmlad pghflytmen ydkenlteem
     2881 inklkvyied pnfqpskiey vskacyslcl wvhamynyyf vnqkvkpkme alakaeealv
     2941 etertllaai qrlqeveagv eklrnllree eekkaelerq kqlcedrmgr avrlidglag
     3001 eqirwkstvv elntslhnai gdillasgai ayltpftdky rdslltswqk llegvphtpg
     3061 sdpvstlgdq veirrwqieg lprdmlsven avlamhsnrw plfidpqaqa nkwirsmyke
     3121 sgisvakmsd kdilrvletc vrfgrvclie nvgieleagl dpillrslfe hggqwcvkig
     3181 etivpyspdf rlflttrlsn phytpdvsvk illvnfaltt tgledqvlsl vviqerpdle
     3241 qtrnalivsn aemrrelkei edrilyrlsv segsavddmd liltleaskv kseeikikmk
     3301 eaeitqadie vtrsmyipva vrgqllffcl sdlqhidtmy qyslewfvdi fnhsiaatak
     3361 sndiqervqn inrnftfslf snvcrslfer hklhfaflvc aririhgnli dhtewrhfla
     3421 gpepyeegpn pspdwitprc wkeiqalenl skfnefvesf khamsqfkav fdaqeahlap
     3481 fpepwqtkld dfqkmlvlkc lrpdkvtnam qsylakhlgr efvepqttel savysesspt
     3541 tpivfilstg tdpaaelykf adrlkmgnkl hpislgqgqg praeamlkfa sehgywcffq
     3601 nchlapswmp dmesvveals raknhrdfrl wltsapspdf pvsilqnstk mtiepprgik
     3661 anmfrayltq viemtaflqs ehpkaaqfkw lvfslclfhs vllerrkfgp lgfnipyeft
     3721 dgdlticisq lhmflleydt vpfkvltyta ghinyggrit ddwdrrcvlt ilqdfyrpqi
     3781 lsptyvfdee khyyqlveta tfdeyieyik tfplndepsm fgmhpnadis faqaetytcl
     3841 dtllalqprq igvagasaee vttqlaedml seipqifdlf tlqtkypvly eesfntvllq
     3901 eairyngllh vvkttlmdll kalkglvvms gqletlftsl ynnripqiwq dkgypslkpl
     3961 gswfldlkdr iaflksweed gipaafwisg fyfpqafltg tlqnfarkhi vsidtinfsf
     4021 kvlqtkpmrr pndgcvvygl flegcrwggh yldeslpkel ytnmppilll pevhhkepeg
     4081 iyscpvyktt nragtlsttg hstnfvltme ipshkpqahw ikrgvalfca ldy
//
LOCUS       XP_034195946            1985 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611782 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195946
VERSION     XP_034195946.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340055.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195946.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1985
                     /product="uncharacterized protein LOC117611782 isoform X1"
                     /calculated_mol_wt=231105
     Region          87..148
                     /region_name="cc_Cep135_MBD"
                     /note="coiled-coil microtubule binding domain of
                     centrosomal protein of 135 kDa (Cep135) and similar
                     proteins; cd22292"
                     /db_xref="CDD:412088"
     Site            order(88,91..92,95..96,98..99,102..103,105..106,109..110,
                     112..113,116..117,119..120,123..124,126..127,130..131,
                     133..134,137..138)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:412088"
     Region          292..1144
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1985
                     /gene="LOC117611782"
                     /coded_by="XM_034340055.2:378..6335"
                     /db_xref="GeneID:117611782"
ORIGIN      
        1 mkmsmeeeyn vaaryrtvrk hldslgykqa ltvdglplie klladllqtt eslkhfksia
       61 qenieahvel ksiidpykcd nvrlvqecnq lhldlikeke shqkqikdlk keiyklehec
      121 ndlqlassrn lhrikeleie sskkskrile lqgkclkpti snvglaskkr pvfplrrpvi
      181 egeplprtgs ksknplpnls tvepkvvdll smadhkmncl shevtklkge lslqtenvht
      241 lksqlatkek eimrlkkmld ggrpytavlk dclckktdkd nvdvnelkil qqekleleqq
      301 lkealnkqhd amtqalklad rneelekelr didhialave adcnstvket nkrvsklqqk
      361 leevmtqvhv legeltverr evqelkadle vcrmeknnik htlestldek kqmtdrinel
      421 ivietnlnde ierlarenek qrqdiiqlqy snkllkdqmn mmeilkdndi inikrtrekg
      481 krnddnngqn gieihethke keiqnengvw ynnvlrqlne ketqinnlqq tveqlgrerd
      541 fyrdeynkik dyhnktidkd hadlwtrire lklqltekes avdelqrekk elyrekvnle
      601 mrlknykaeh ksscrpcnvc krgnscncsl savkdisktk siierleher dvaradverl
      661 ieerdalrer lkmaaeahts eqhrlrenlt dvearlkqie kerqellltq gsrratikgl
      721 edqlqdlree lkrtkqelgt qrtqyfqlra lqdqtdqaln daqnqltqse selnkafdrs
      781 ramerqqlql esqvkelqqe icnlktkmth ldhekdqllm vldekteria alekeimlkd
      841 qqvmgseqqi relqqknemt idqsaeqdrq lrmmqleven lqrqlhtasy drenaiqenq
      901 rvqddlaavt cevrnlqrel dasraecsdl krqlqtyvge vrraeellnr kenertemln
      961 hfrslsleat vlennnhsle seaaeargal hsardrlldf erqladkdsl irgyetqise
     1021 ltqnvasmet qlrqqgeqrh rteadlnavr dlcvkldqqk dtlmqqmddk daikaqydmq
     1081 iaklkaeqva iqdqmnrdrv tverlemlle qarqesitaq annqelqnem srlrqkmsel
     1141 qnklssessk lrqyqtqaae yckqiselrr qvtnerfdra rkdeesrrhs eglpdslvqt
     1201 phptnqnpne sndtgfatrr ylqneehdgt tdrsgmkmvd diavsrfgyl kvesnyrcnn
     1261 rpkeiqrdvp ciniilrsdc kmhsnqynyv qkenaqnenk peprnnedlv ktnvqrptvs
     1321 glnspgtkyn styfntdnhf dvlqtlgmky nytafdienk lnvsqsvgvq nkaqcnqgtk
     1381 qlasnstski ryssplfsyy pvhsteisnd skaldnaakk rlafsknsnv vlndkianiv
     1441 ctnksynisk nnydkytsle estinktisr nkkltkflhq ddinfctdtt ctktncsast
     1501 tkrenkhkst htdikcmhdv ttstllkste nfhynrerek kcintidtal dtvqnmlkgv
     1561 kkfedkdidk qnaenyhyqe vrcknmgcny lnavhdvais kykqncanla eiikpeyvkm
     1621 lqeikeqtma leeqliimnk nmkikkrtie klmsapeyqd tynteqqrdi itqnpekrni
     1681 cvqepsests yvncmqyttl kpekdndeqd ikkqdvalkk skhkreveqn cknfteqqen
     1741 kdhrkdinnf krthnvqiqc irsfhiidas sfksfststs kkvnpannkn kvyyisrrvq
     1801 ssltskenlr sqrinktmld kliinavemn nkgindeqes ltyeekaimm lllaksisnk
     1861 clkskhkkse linfkpkcii dscipcdqrl vkcklskrkm qipvvfkqsk pikyndeqni
     1921 ysnqnscecl plqsennlvt tvctqssnlq ittatsvsct sfnnriivdn vieerrtrgd
     1981 acils
//
LOCUS       XP_034195948            1964 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611782 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195948
VERSION     XP_034195948.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340057.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195948.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1964
                     /product="uncharacterized protein LOC117611782 isoform X2"
                     /calculated_mol_wt=228770
     Region          87..148
                     /region_name="cc_Cep135_MBD"
                     /note="coiled-coil microtubule binding domain of
                     centrosomal protein of 135 kDa (Cep135) and similar
                     proteins; cd22292"
                     /db_xref="CDD:412088"
     Site            order(88,91..92,95..96,98..99,102..103,105..106,109..110,
                     112..113,116..117,119..120,123..124,126..127,130..131,
                     133..134,137..138)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:412088"
     Region          271..1119
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1964
                     /gene="LOC117611782"
                     /coded_by="XM_034340057.2:378..6272"
                     /db_xref="GeneID:117611782"
ORIGIN      
        1 mkmsmeeeyn vaaryrtvrk hldslgykqa ltvdglplie klladllqtt eslkhfksia
       61 qenieahvel ksiidpykcd nvrlvqecnq lhldlikeke shqkqikdlk keiyklehec
      121 ndlqlassrn lhrikeleie sskkskrile lqgkclkpti snvglaskkr pvfplrrpvi
      181 egeplprtgs ksknplpnls tvepkvvdll smadhkmncl shelatkeke imrlkkmldg
      241 grpytavlkd clckktdkdn vdvnelkilq qekleleqql kealnkqhda mtqalkladr
      301 neelekelrd idhialavea dcnstvketn krvsklqqkl eevmtqvhvl egeltverre
      361 vqelkadlev crmeknnikh tlestldekk qmtdrineli vietnlndei erlarenekq
      421 rqdiiqlqys nkllkdqmnm meilkdndii nikrtrekgk rnddnngqng ieihethkek
      481 eiqnengvwy nnvlrqlnek etqinnlqqt veqlgrerdf yrdeynkikd yhnktidkdh
      541 adlwtrirel klqltekesa vdelqrekke lyrekvnlem rlknykaehk sscrpcnvck
      601 rgnscncsls avkdisktks iierleherd varadverli eerdalrerl kmaaeahtse
      661 qhrlrenltd vearlkqiek erqellltqg srratikgle dqlqdlreel krtkqelgtq
      721 rtqyfqlral qdqtdqalnd aqnqltqses elnkafdrsr amerqqlqle sqvkelqqei
      781 cnlktkmthl dhekdqllmv ldekteriaa lekeimlkdq qvmgseqqir elqqknemti
      841 dqsaeqdrql rmmqlevenl qrqlhtasyd renaiqenqr vqddlaavtc evrnlqreld
      901 asraecsdlk rqlqtyvgev rraeellnrk enertemlnh frslsleatv lennnhsles
      961 eaaeargalh sardrlldfe rqladkdsli rgyetqisel tqnvasmetq lrqqgeqrhr
     1021 teadlnavrd lcvkldqqkd tlmqqmddkd aikaqydmqi aklkaeqvai qdqmnrdrvt
     1081 verlemlleq arqesitaqa nnqelqnems rlrqkmselq nklssesskl rqyqtqaaey
     1141 ckqiselrrq vtnerfdrar kdeesrrhse glpdslvqtp hptnqnpnes ndtgfatrry
     1201 lqneehdgtt drsgmkmvdd iavsrfgylk vesnyrcnnr pkeiqrdvpc iniilrsdck
     1261 mhsnqynyvq kenaqnenkp eprnnedlvk tnvqrptvsg lnspgtkyns tyfntdnhfd
     1321 vlqtlgmkyn ytafdienkl nvsqsvgvqn kaqcnqgtkq lasnstskir yssplfsyyp
     1381 vhsteisnds kaldnaakkr lafsknsnvv lndkianivc tnksyniskn nydkytslee
     1441 stinktisrn kkltkflhqd dinfctdttc tktncsastt krenkhksth tdikcmhdvt
     1501 tstllksten fhynrerekk cintidtald tvqnmlkgvk kfedkdidkq naenyhyqev
     1561 rcknmgcnyl navhdvaisk ykqncanlae iikpeyvkml qeikeqtmal eeqliimnkn
     1621 mkikkrtiek lmsapeyqdt ynteqqrdii tqnpekrnic vqepsestsy vncmqyttlk
     1681 pekdndeqdi kkqdvalkks khkreveqnc knfteqqenk dhrkdinnfk rthnvqiqci
     1741 rsfhiidass fksfststsk kvnpannknk vyyisrrvqs sltskenlrs qrinktmldk
     1801 liinavemnn kgindeqesl tyeekaimml llaksisnkc lkskhkksel infkpkciid
     1861 scipcdqrlv kcklskrkmq ipvvfkqskp ikyndeqniy snqnsceclp lqsennlvtt
     1921 vctqssnlqi ttatsvscts fnnriivdnv ieerrtrgda cils
//
LOCUS       XP_034195949            1297 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611782 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195949
VERSION     XP_034195949.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340058.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195949.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1297
                     /product="uncharacterized protein LOC117611782 isoform X3"
                     /calculated_mol_wt=151637
     Region          83..898
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          575..>1171
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1297
                     /gene="LOC117611782"
                     /coded_by="XM_034340058.2:379..4272"
                     /db_xref="GeneID:117611782"
ORIGIN      
        1 mkmsmeeeyn vaaryrtvrk hldslgykqa ltvdglplie klladllqtt eslkhfksia
       61 qenieahvel ksiidpykcd nvrlvqecnq lhldlikeke shqkqikdlk keiyklehec
      121 ndlqlassrn lhrikeleie sskkskrile lqgkclkpti snvglaskkr pvfplrrpvi
      181 egeplprtgs ksknplpnls tvepkvvdll smadhkmncl shevtklkge lslqtenvht
      241 lksqlatkek eimrlkkmld ggrpytavlk dclckktdkd nvdvnelkil qqekleleqq
      301 lkealnkqhd amtqalklad rneelekelr didhialave adcnstvket nkrvsklqqk
      361 leevmtqvhv legeltverr evqelkadle vcrmeknnik htlestldek kqmtdrinel
      421 ivietnlnde ierlarenek qrqdiiqlqy snkllkdqmn mmeilkdndi inikrtrekg
      481 krnddnngqn gieihethke keiqnengvw ynnvlrqlne ketqinnlqq tveqlgrerd
      541 fyrdeynkik dyhnktidkd hadlwtrire lklqltekes avdelqrekk elyrekvnle
      601 mrlknykaeh ksscrpcnvc krgnscncsl savkdisktk siierleher dvaradverl
      661 ieerdalrer lkmaaeahts eqhrlrenlt dvearlkqie kerqellltq gsrratikgl
      721 edqlqdlree lkrtkqelgt qrtqyfqlra lqdqtdqaln daqnqltqse selnkafdrs
      781 ramerqqlql esqvkelqqe icnlktkmth ldhekdqllm vldekteria alekeimlkd
      841 qqvmgseqqi relqqknemt idqsaeqdrq lrmmqleven lqrqlhtasy drenaiqenq
      901 rvqddlaavt cevrnlqrel dasraecsdl krqlqtyvge vrraeellnr kenertemln
      961 hfrslsleat vlennnhsle seaaeargal hsardrlldf erqladkdsl irgyetqise
     1021 ltqnvasmet qlrqqgeqrh rteadlnavr dlcvkldqqk dtlmqqmddk daikaqydmq
     1081 iaklkaeqva iqdqmnrdrv tverlemlle qarqesitaq annqelqnem srlrqkmsel
     1141 qnklssessk lrqyqtqaae yckqiselrr qvtnerfdra rkdeesrrhs eglpdslvqt
     1201 phptnqnpne sndtgfatrr qvdwlkkpyt aipkysrils parsvnerhs fpmdckclps
     1261 rtifhesssn klplfpsvsn neadvsrrnn sensnkr
//
LOCUS       XP_034195950            1237 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611782 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195950
VERSION     XP_034195950.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340059.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1237
                     /product="uncharacterized protein LOC117611782 isoform X4"
                     /calculated_mol_wt=144593
     Region          83..898
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          575..>1171
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1237
                     /gene="LOC117611782"
                     /coded_by="XM_034340059.2:377..4090"
                     /db_xref="GeneID:117611782"
ORIGIN      
        1 mkmsmeeeyn vaaryrtvrk hldslgykqa ltvdglplie klladllqtt eslkhfksia
       61 qenieahvel ksiidpykcd nvrlvqecnq lhldlikeke shqkqikdlk keiyklehec
      121 ndlqlassrn lhrikeleie sskkskrile lqgkclkpti snvglaskkr pvfplrrpvi
      181 egeplprtgs ksknplpnls tvepkvvdll smadhkmncl shevtklkge lslqtenvht
      241 lksqlatkek eimrlkkmld ggrpytavlk dclckktdkd nvdvnelkil qqekleleqq
      301 lkealnkqhd amtqalklad rneelekelr didhialave adcnstvket nkrvsklqqk
      361 leevmtqvhv legeltverr evqelkadle vcrmeknnik htlestldek kqmtdrinel
      421 ivietnlnde ierlarenek qrqdiiqlqy snkllkdqmn mmeilkdndi inikrtrekg
      481 krnddnngqn gieihethke keiqnengvw ynnvlrqlne ketqinnlqq tveqlgrerd
      541 fyrdeynkik dyhnktidkd hadlwtrire lklqltekes avdelqrekk elyrekvnle
      601 mrlknykaeh ksscrpcnvc krgnscncsl savkdisktk siierleher dvaradverl
      661 ieerdalrer lkmaaeahts eqhrlrenlt dvearlkqie kerqellltq gsrratikgl
      721 edqlqdlree lkrtkqelgt qrtqyfqlra lqdqtdqaln daqnqltqse selnkafdrs
      781 ramerqqlql esqvkelqqe icnlktkmth ldhekdqllm vldekteria alekeimlkd
      841 qqvmgseqqi relqqknemt idqsaeqdrq lrmmqleven lqrqlhtasy drenaiqenq
      901 rvqddlaavt cevrnlqrel dasraecsdl krqlqtyvge vrraeellnr kenertemln
      961 hfrslsleat vlennnhsle seaaeargal hsardrlldf erqladkdsl irgyetqise
     1021 ltqnvasmet qlrqqgeqrh rteadlnavr dlcvkldqqk dtlmqqmddk daikaqydmq
     1081 iaklkaeqva iqdqmnrdrv tverlemlle qarqesitaq annqelqnem srlrqkmsel
     1141 qnklssessk lrqyqtqaae yckqiselrr qvtnerfdra rkdeesrrhs eglpdslvqt
     1201 phptnqnpne sndtgfatrr slnstpdsfn vdlnvvi
//
LOCUS       XP_034195951            1205 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611782 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195951
VERSION     XP_034195951.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340060.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1205
                     /product="uncharacterized protein LOC117611782 isoform X5"
                     /calculated_mol_wt=141093
     Region          83..898
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          575..>1171
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1205
                     /gene="LOC117611782"
                     /coded_by="XM_034340060.2:376..3993"
                     /db_xref="GeneID:117611782"
ORIGIN      
        1 mkmsmeeeyn vaaryrtvrk hldslgykqa ltvdglplie klladllqtt eslkhfksia
       61 qenieahvel ksiidpykcd nvrlvqecnq lhldlikeke shqkqikdlk keiyklehec
      121 ndlqlassrn lhrikeleie sskkskrile lqgkclkpti snvglaskkr pvfplrrpvi
      181 egeplprtgs ksknplpnls tvepkvvdll smadhkmncl shevtklkge lslqtenvht
      241 lksqlatkek eimrlkkmld ggrpytavlk dclckktdkd nvdvnelkil qqekleleqq
      301 lkealnkqhd amtqalklad rneelekelr didhialave adcnstvket nkrvsklqqk
      361 leevmtqvhv legeltverr evqelkadle vcrmeknnik htlestldek kqmtdrinel
      421 ivietnlnde ierlarenek qrqdiiqlqy snkllkdqmn mmeilkdndi inikrtrekg
      481 krnddnngqn gieihethke keiqnengvw ynnvlrqlne ketqinnlqq tveqlgrerd
      541 fyrdeynkik dyhnktidkd hadlwtrire lklqltekes avdelqrekk elyrekvnle
      601 mrlknykaeh ksscrpcnvc krgnscncsl savkdisktk siierleher dvaradverl
      661 ieerdalrer lkmaaeahts eqhrlrenlt dvearlkqie kerqellltq gsrratikgl
      721 edqlqdlree lkrtkqelgt qrtqyfqlra lqdqtdqaln daqnqltqse selnkafdrs
      781 ramerqqlql esqvkelqqe icnlktkmth ldhekdqllm vldekteria alekeimlkd
      841 qqvmgseqqi relqqknemt idqsaeqdrq lrmmqleven lqrqlhtasy drenaiqenq
      901 rvqddlaavt cevrnlqrel dasraecsdl krqlqtyvge vrraeellnr kenertemln
      961 hfrslsleat vlennnhsle seaaeargal hsardrlldf erqladkdsl irgyetqise
     1021 ltqnvasmet qlrqqgeqrh rteadlnavr dlcvkldqqk dtlmqqmddk daikaqydmq
     1081 iaklkaeqva iqdqmnrdrv tverlemlle qarqesitaq annqelqnem srlrqkmsel
     1141 qnklssessk lrqyqtqaae yckqiselrr qvtnerfdra rkdeesrrsl nstpdsfnvd
     1201 lnvvi
//
LOCUS       XP_034195952            1867 aa            linear   INV 03-SEP-2025
DEFINITION  brahma associated protein 170kD isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195952
VERSION     XP_034195952.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340061.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195952.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1867
                     /product="brahma associated protein 170kD isoform X1"
                     /calculated_mol_wt=204652
     Region          15..102
                     /region_name="ARID_ARID2"
                     /note="ARID/BRIGHT DNA binding domain of AT-rich
                     interactive domain-containing protein 2 (ARID2) and
                     similar proteins; cd16866"
                     /db_xref="CDD:350630"
     Site            order(36..42,67,69..70,73,84,86..88,90)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350630"
     Region          <546..796
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          626..706
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     Region          <1399..1585
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     CDS             1..1867
                     /gene="Bap170"
                     /coded_by="XM_034340061.2:1349..6952"
                     /db_xref="GeneID:117611783"
ORIGIN      
        1 makilnkdpv tyererenfl kdlrhfhetr gtpfkkipkv sgkdidlyll yvvvtahggw
       61 irvnlrnewt slceqfhlpk gcvnsgvglk qiylryldry ekvhflgedg qqaddddeds
      121 rhrkwsaral hsvpltynhh qhnvaeslrd ynglssdlyk psnydklals llsplpneqd
      181 fainvctlls negkhilrld kyprlvnill ahagvfdspg trqlfievys rvrnysinsf
      241 wsdvldsqdv idltnerafm kkptaspqtf srrkilekek qnkstalstt enddastsme
      301 iegvpqdcpr ldptgshqdd nlrgdqnqns ikfeeedkdl fcvgrtlgtq dpygqrvlqi
      361 asilrnlsft penaavlgrn rcflrfvllc vrarwsnlhq lgfdilgnia neiilkeage
      421 ritdvvlscv akgiesqdrf ivisclevln kisqqdsnee ivtfglednv yelicrflal
      481 ndiallvytl eclyaltslg erpctsvarv rgaidtlval vtveaqsygp kacilmrvie
      541 tvstvaappn ttqntpitat apaatvsssv pstpatvtat papvspapsr pttpaatvtk
      601 stthkavean nsiqqhahqq iiqeneqfal swlratfept pgvrieqeel ykkylgcctk
      661 igrrgviapl hfprcvrsvf ggsvgpnplk getsgtqyye girvratppq vtypsqtavg
      721 tntapitavn atpvkvlpvq qrtiknispa pdstaldnpa sgppripapa spilkaqlsa
      781 ppkppsntsn tanqsqnpvt kvdsksqvsv shphlsqall ssgsqiqtqq qqlqqqqpqq
      841 vqhqpqqpqq vqqqsqtiqv vakednrsgt tsssiiksll atkvtvssdc mpstaatcvs
      901 tptacvtntt asiassisan qlitsnqvaq rqqqqrllqq qlinvsqppt tttstilskt
      961 pvnskqkpai tpipakkiqr lngakfimtn ncdkyfvfqt evsdnenvnq iatsttssiv
     1021 vlnstenhis stikvcrpps ttvttvnktn qrstctsiae dsdstnnsla sssgiggsrd
     1081 csgvgveedn sltsfegill ngapsnmdid aqddgsskds ssviskekpl qsmmladlle
     1141 rkvdkepiln gvlgknsine kgmdlvenhi kkvlkesptd ikvklevees nvvqtevsed
     1201 tlieaprgik raasesdevd vkkvkysngt mspdpavdst taestvssik seeqdddkds
     1261 ekatvsstaa nlyaalaadc iedetdlden invnastgts paikeephif vnqinqqqql
     1321 qqqqqqklql qqqlqqqqqq qqqqqqqqqq qqqqqhqqqq qhqqqhhhhh hhhhhqlqqp
     1381 qqtqtqlqqq qhqhqqliva asrqivvqqt iqpnnqviip agqvkgrqaq pqpqvllqqg
     1441 aggqlqyvvs ggvpgqnyvl aqpqttlvqg qaqtvlvaqt tqqqgtgakt iiilqpqaaa
     1501 qptqqkmvav tpqgqqvvvt qvsrpilqsp algnippplv ptsgtipqts iivnssvaqt
     1561 npntvtvtip amaqqtpvst svpsrvvtpt pastppptrp ttphqaaath glpakqpakl
     1621 iktvssstst epeskpstsq nqpektitik idpnaylcew rgclrqfktp hevylhvcea
     1681 hcptggeeil clwascdalk rrrfslmthl ydrhcnaetm smrrkqltvt gktevststp
     1741 ptphhpgyap naafhaikrh alefvnpkel mqrptkpaaa tsssssprpg qnpppeqddn
     1801 egpvtksirl taalilrnlv iysthgrrhl rayephlagv alsnvessrt iaqvlydmnd
     1861 qsnsnhr
//
LOCUS       XP_034195953            1862 aa            linear   INV 03-SEP-2025
DEFINITION  brahma associated protein 170kD isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195953
VERSION     XP_034195953.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340062.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195953.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1862
                     /product="brahma associated protein 170kD isoform X2"
                     /calculated_mol_wt=203967
     Region          15..102
                     /region_name="ARID_ARID2"
                     /note="ARID/BRIGHT DNA binding domain of AT-rich
                     interactive domain-containing protein 2 (ARID2) and
                     similar proteins; cd16866"
                     /db_xref="CDD:350630"
     Site            order(36..42,67,69..70,73,84,86..88,90)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350630"
     Region          <546..796
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          626..706
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     Region          <1394..1580
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     CDS             1..1862
                     /gene="Bap170"
                     /coded_by="XM_034340062.2:1228..6816"
                     /db_xref="GeneID:117611783"
ORIGIN      
        1 makilnkdpv tyererenfl kdlrhfhetr gtpfkkipkv sgkdidlyll yvvvtahggw
       61 irvnlrnewt slceqfhlpk gcvnsgvglk qiylryldry ekvhflgedg qqaddddeds
      121 rhrkwsaral hsvpltynhh qhnvaeslrd ynglssdlyk psnydklals llsplpneqd
      181 fainvctlls negkhilrld kyprlvnill ahagvfdspg trqlfievys rvrnysinsf
      241 wsdvldsqdv idltnerafm kkptaspqtf srrkilekek qnkstalstt enddastsme
      301 iegvpqdcpr ldptgshqdd nlrgdqnqns ikfeeedkdl fcvgrtlgtq dpygqrvlqi
      361 asilrnlsft penaavlgrn rcflrfvllc vrarwsnlhq lgfdilgnia neiilkeage
      421 ritdvvlscv akgiesqdrf ivisclevln kisqqdsnee ivtfglednv yelicrflal
      481 ndiallvytl eclyaltslg erpctsvarv rgaidtlval vtveaqsygp kacilmrvie
      541 tvstvaappn ttqntpitat apaatvsssv pstpatvtat papvspapsr pttpaatvtk
      601 stthkavean nsiqqhahqq iiqeneqfal swlratfept pgvrieqeel ykkylgcctk
      661 igrrgviapl hfprcvrsvf ggsvgpnplk getsgtqyye girvratppq vtypsqtavg
      721 tntapitavn atpvkvlpvq qrtiknispa pdstaldnpa sgppripapa spilkaqlsa
      781 ppkppsntsn tanqsqnpvt kvdsksqvsv shphlsqall ssgsqiqtqq qqlqqqqpqq
      841 vqhqpqqpqq vqqqsqtiqv vakednrsgt tsssiiksll atkvtvssdc mpstaatcvs
      901 tptacvtntt asiassisan qlitsnqvaq rqqqqrllqq qlinvsqppt tttstilskt
      961 pvnskqkpai tpipakkiqr lngakfimtn ncdktevsdn envnqiatst tssivvlnst
     1021 enhisstikv crppsttvtt vnktnqrstc tsiaedsdst nnslasssgi ggsrdcsgvg
     1081 veednsltsf egillngaps nmdidaqddg sskdsssvis kekplqsmml adllerkvdk
     1141 epilngvlgk nsinekgmdl venhikkvlk esptdikvkl eveesnvvqt evsedtliea
     1201 prgikraase sdevdvkkvk ysngtmspdp avdsttaest vssikseeqd ddkdsekatv
     1261 sstaanlyaa laadciedet dldeninvna stgtspaike ephifvnqin qqqqlqqqqq
     1321 qklqlqqqlq qqqqqqqqqq qqqqqqqqqq hqqqqqhqqq hhhhhhhhhh qlqqpqqtqt
     1381 qlqqqqhqhq qlivaasrqi vvqqtiqpnn qviipagqvk grqaqpqpqv llqqgaggql
     1441 qyvvsggvpg qnyvlaqpqt tlvqgqaqtv lvaqttqqqg tgaktiiilq pqaaaqptqq
     1501 kmvavtpqgq qvvvtqvsrp ilqspalgni ppplvptsgt ipqtsiivns svaqtnpntv
     1561 tvtipamaqq tpvstsvpsr vvtptpastp pptrpttphq aaathglpak qpakliktvs
     1621 sststepesk pstsqnqpek titikidpna ylcewrgclr qfktphevyl hvceahcptg
     1681 geeilclwas cdalkrrrfs lmthlydrhc naetmsmrrk qltvtgktev ststpptphh
     1741 pgyapnaafh aikrhalefv npkelmqrpt kpaaatssss sprpgqnppp eqddnegpvt
     1801 ksirltaali lrnlviysth grrhlrayep hlagvalsnv essrtiaqvl ydmndqsnsn
     1861 hr
//
LOCUS       XP_034195956            1823 aa            linear   INV 03-SEP-2025
DEFINITION  brahma associated protein 170kD isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195956
VERSION     XP_034195956.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340065.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195956.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1823
                     /product="brahma associated protein 170kD isoform X3"
                     /calculated_mol_wt=200334
     Region          15..102
                     /region_name="ARID_ARID2"
                     /note="ARID/BRIGHT DNA binding domain of AT-rich
                     interactive domain-containing protein 2 (ARID2) and
                     similar proteins; cd16866"
                     /db_xref="CDD:350630"
     Site            order(36..42,67,69..70,73,84,86..88,90)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350630"
     Region          <546..796
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          626..706
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     Region          <1355..1541
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     CDS             1..1823
                     /gene="Bap170"
                     /coded_by="XM_034340065.2:1350..6821"
                     /db_xref="GeneID:117611783"
ORIGIN      
        1 makilnkdpv tyererenfl kdlrhfhetr gtpfkkipkv sgkdidlyll yvvvtahggw
       61 irvnlrnewt slceqfhlpk gcvnsgvglk qiylryldry ekvhflgedg qqaddddeds
      121 rhrkwsaral hsvpltynhh qhnvaeslrd ynglssdlyk psnydklals llsplpneqd
      181 fainvctlls negkhilrld kyprlvnill ahagvfdspg trqlfievys rvrnysinsf
      241 wsdvldsqdv idltnerafm kkptaspqtf srrkilekek qnkstalstt enddastsme
      301 iegvpqdcpr ldptgshqdd nlrgdqnqns ikfeeedkdl fcvgrtlgtq dpygqrvlqi
      361 asilrnlsft penaavlgrn rcflrfvllc vrarwsnlhq lgfdilgnia neiilkeage
      421 ritdvvlscv akgiesqdrf ivisclevln kisqqdsnee ivtfglednv yelicrflal
      481 ndiallvytl eclyaltslg erpctsvarv rgaidtlval vtveaqsygp kacilmrvie
      541 tvstvaappn ttqntpitat apaatvsssv pstpatvtat papvspapsr pttpaatvtk
      601 stthkavean nsiqqhahqq iiqeneqfal swlratfept pgvrieqeel ykkylgcctk
      661 igrrgviapl hfprcvrsvf ggsvgpnplk getsgtqyye girvratppq vtypsqtavg
      721 tntapitavn atpvkvlpvq qrtiknispa pdstaldnpa sgppripapa spilkaqlsa
      781 ppkppsntsn tanqsqnpvt kvdsksqvsv shphlsqall ssgsqiqtqq qqlqqqqpqq
      841 vqhqpqqpqq vqqqsqtiqv vakednrsgt tsssiiksll atkvaqrqqq qrllqqqlin
      901 vsqpptttts tilsktpvns kqkpaitpip akkiqrlnga kfimtnncdk yfvfqtevsd
      961 nenvnqiats ttssivvlns tenhisstik vcrppsttvt tvnktnqrst ctsiaedsds
     1021 tnnslasssg iggsrdcsgv gveednslts fegillngap snmdidaqdd gsskdsssvi
     1081 skekplqsmm ladllerkvd kepilngvlg knsinekgmd lvenhikkvl kesptdikvk
     1141 leveesnvvq tevsedtlie aprgikraas esdevdvkkv kysngtmspd pavdsttaes
     1201 tvssikseeq dddkdsekat vsstaanlya alaadciede tdldeninvn astgtspaik
     1261 eephifvnqi nqqqqlqqqq qqklqlqqql qqqqqqqqqq qqqqqqqqqq qhqqqqqhqq
     1321 qhhhhhhhhh hqlqqpqqtq tqlqqqqhqh qqlivaasrq ivvqqtiqpn nqviipagqv
     1381 kgrqaqpqpq vllqqgaggq lqyvvsggvp gqnyvlaqpq ttlvqgqaqt vlvaqttqqq
     1441 gtgaktiiil qpqaaaqptq qkmvavtpqg qqvvvtqvsr pilqspalgn ippplvptsg
     1501 tipqtsiivn ssvaqtnpnt vtvtipamaq qtpvstsvps rvvtptpast ppptrpttph
     1561 qaaathglpa kqpakliktv ssststepes kpstsqnqpe ktitikidpn aylcewrgcl
     1621 rqfktphevy lhvceahcpt ggeeilclwa scdalkrrrf slmthlydrh cnaetmsmrr
     1681 kqltvtgkte vststpptph hpgyapnaaf haikrhalef vnpkelmqrp tkpaaatsss
     1741 ssprpgqnpp peqddnegpv tksirltaal ilrnlviyst hgrrhlraye phlagvalsn
     1801 vessrtiaqv lydmndqsns nhr
//
LOCUS       XP_034195957            1795 aa            linear   INV 03-SEP-2025
DEFINITION  brahma associated protein 170kD isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195957
VERSION     XP_034195957.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340066.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195957.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1795
                     /product="brahma associated protein 170kD isoform X4"
                     /calculated_mol_wt=197236
     Region          15..102
                     /region_name="ARID_ARID2"
                     /note="ARID/BRIGHT DNA binding domain of AT-rich
                     interactive domain-containing protein 2 (ARID2) and
                     similar proteins; cd16866"
                     /db_xref="CDD:350630"
     Site            order(36..42,67,69..70,73,84,86..88,90)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:350630"
     Region          <541..>612
                     /region_name="PRK10856"
                     /note="cytoskeleton protein RodZ"
                     /db_xref="CDD:236776"
     Region          626..706
                     /region_name="RFX_DNA_binding"
                     /note="RFX DNA-binding domain; pfam02257"
                     /db_xref="CDD:460512"
     Region          <1327..1513
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     CDS             1..1795
                     /gene="Bap170"
                     /coded_by="XM_034340066.2:1282..6669"
                     /db_xref="GeneID:117611783"
ORIGIN      
        1 makilnkdpv tyererenfl kdlrhfhetr gtpfkkipkv sgkdidlyll yvvvtahggw
       61 irvnlrnewt slceqfhlpk gcvnsgvglk qiylryldry ekvhflgedg qqaddddeds
      121 rhrkwsaral hsvpltynhh qhnvaeslrd ynglssdlyk psnydklals llsplpneqd
      181 fainvctlls negkhilrld kyprlvnill ahagvfdspg trqlfievys rvrnysinsf
      241 wsdvldsqdv idltnerafm kkptaspqtf srrkilekek qnkstalstt enddastsme
      301 iegvpqdcpr ldptgshqdd nlrgdqnqns ikfeeedkdl fcvgrtlgtq dpygqrvlqi
      361 asilrnlsft penaavlgrn rcflrfvllc vrarwsnlhq lgfdilgnia neiilkeage
      421 ritdvvlscv akgiesqdrf ivisclevln kisqqdsnee ivtfglednv yelicrflal
      481 ndiallvytl eclyaltslg erpctsvarv rgaidtlval vtveaqsygp kacilmrvie
      541 tvstvaappn ttqntpitat apaatvsssv pstpatvtat papvspapsr pttpaatvtk
      601 stthkavean nsiqqhahqq iiqeneqfal swlratfept pgvrieqeel ykkylgcctk
      661 igrrgviapl hfprcvrsvf ggsvgpnplk getsgtqyye girvratppq vtypsqtavg
      721 tntapitavn atpvkvsvsh phlsqallss gsqiqtqqqq lqqqqpqqvq hqpqqpqqvq
      781 qqsqtiqvva kednrsgtts ssiiksllat kvtvssdcmp staatcvstp tacvtnttas
      841 iassisanql itsnqvaqrq qqqrllqqql invsqppttt tstilsktpv nskqkpaitp
      901 ipakkiqrln gakfimtnnc dkyfvfqtev sdnenvnqia tsttssivvl nstenhisst
      961 ikvcrppstt vttvnktnqr stctsiaeds dstnnslass sgiggsrdcs gvgveednsl
     1021 tsfegillng apsnmdidaq ddgsskdsss viskekplqs mmladllerk vdkepilngv
     1081 lgknsinekg mdlvenhikk vlkesptdik vkleveesnv vqtevsedtl ieaprgikra
     1141 asesdevdvk kvkysngtms pdpavdstta estvssikse eqdddkdsek atvsstaanl
     1201 yaalaadcie detdldenin vnastgtspa ikeephifvn qinqqqqlqq qqqqklqlqq
     1261 qlqqqqqqqq qqqqqqqqqq qqqhqqqqqh qqqhhhhhhh hhhqlqqpqq tqtqlqqqqh
     1321 qhqqlivaas rqivvqqtiq pnnqviipag qvkgrqaqpq pqvllqqgag gqlqyvvsgg
     1381 vpgqnyvlaq pqttlvqgqa qtvlvaqttq qqgtgaktii ilqpqaaaqp tqqkmvavtp
     1441 qgqqvvvtqv srpilqspal gnippplvpt sgtipqtsii vnssvaqtnp ntvtvtipam
     1501 aqqtpvstsv psrvvtptpa stppptrptt phqaaathgl pakqpaklik tvssststep
     1561 eskpstsqnq pektitikid pnaylcewrg clrqfktphe vylhvceahc ptggeeilcl
     1621 wascdalkrr rfslmthlyd rhcnaetmsm rrkqltvtgk tevststppt phhpgyapna
     1681 afhaikrhal efvnpkelmq rptkpaaats ssssprpgqn pppeqddneg pvtksirlta
     1741 alilrnlviy sthgrrhlra yephlagval snvessrtia qvlydmndqs nsnhr
//
LOCUS       XP_076546767             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611785 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546767
VERSION     XP_076546767.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690652.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC117611785 isoform X1"
                     /calculated_mol_wt=53442
     Region          72..165
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     Region          270..332
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     Region          <311..399
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     CDS             1..486
                     /gene="LOC117611785"
                     /coded_by="XM_076690652.1:2142..3602"
                     /db_xref="GeneID:117611785"
ORIGIN      
        1 mftmkssfsv rdllvviwft smvqlrarsa impppwadps snpcaaqprg wqllfwppdg
       61 kcykifqigp pcpetmelgp aaggggtvae crcppgtaqs prdalchpiy trascpsgqf
      121 fepvpetsgk srwgvcrepe pctekgevyw prdgkcypkf srgpcpkgei latdedglti
      181 cscsttgelg ryhwpgsvgg chehytkgpc tepgelflpg gscgchsrlp hyheptrmcy
      241 qlggigpclk ghyyvvtnsa snedtarasc vckpghvlyk ngfcyrlytr gpcepgymlm
      301 nstgcapvpc krgrlyipqe ktcykigtrg pcpngqivly dynvrpsidg isyngmcgct
      361 nifkdsekcs eesndscest pgmveinktc yklytqgpct agewlvarrm pkptlweske
      421 seprarcecr pgykritens eiselennnl lspgscqppt vslakflnen vksinlsvre
      481 ielavh
//
LOCUS       XP_034195959             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611785 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195959
VERSION     XP_034195959.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340068.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195959.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC117611785 isoform X1"
                     /calculated_mol_wt=53442
     Region          72..165
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     Region          270..332
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     Region          <311..399
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     CDS             1..486
                     /gene="LOC117611785"
                     /coded_by="XM_034340068.2:580..2040"
                     /db_xref="GeneID:117611785"
ORIGIN      
        1 mftmkssfsv rdllvviwft smvqlrarsa impppwadps snpcaaqprg wqllfwppdg
       61 kcykifqigp pcpetmelgp aaggggtvae crcppgtaqs prdalchpiy trascpsgqf
      121 fepvpetsgk srwgvcrepe pctekgevyw prdgkcypkf srgpcpkgei latdedglti
      181 cscsttgelg ryhwpgsvgg chehytkgpc tepgelflpg gscgchsrlp hyheptrmcy
      241 qlggigpclk ghyyvvtnsa snedtarasc vckpghvlyk ngfcyrlytr gpcepgymlm
      301 nstgcapvpc krgrlyipqe ktcykigtrg pcpngqivly dynvrpsidg isyngmcgct
      361 nifkdsekcs eesndscest pgmveinktc yklytqgpct agewlvarrm pkptlweske
      421 seprarcecr pgykritens eiselennnl lspgscqppt vslakflnen vksinlsvre
      481 ielavh
//
LOCUS       XP_034195960             485 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611785 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195960
VERSION     XP_034195960.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340069.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195960.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..485
                     /product="uncharacterized protein LOC117611785 isoform X2"
                     /calculated_mol_wt=53355
     Region          71..164
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     Region          269..331
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     Region          <310..398
                     /region_name="DUF4789"
                     /note="Domain of unknown function (DUF4789); pfam16033"
                     /db_xref="CDD:435087"
     CDS             1..485
                     /gene="LOC117611785"
                     /coded_by="XM_034340069.2:585..2042"
                     /db_xref="GeneID:117611785"
ORIGIN      
        1 mftmkssfvr dllvviwfts mvqlrarsai mpppwadpss npcaaqprgw qllfwppdgk
       61 cykifqigpp cpetmelgpa aggggtvaec rcppgtaqsp rdalchpiyt rascpsgqff
      121 epvpetsgks rwgvcrepep ctekgevywp rdgkcypkfs rgpcpkgeil atdedgltic
      181 scsttgelgr yhwpgsvggc hehytkgpct epgelflpgg scgchsrlph yheptrmcyq
      241 lggigpclkg hyyvvtnsas nedtarascv ckpghvlykn gfcyrlytrg pcepgymlmn
      301 stgcapvpck rgrlyipqek tcykigtrgp cpngqivlyd ynvrpsidgi syngmcgctn
      361 ifkdsekcse esndscestp gmveinktcy klytqgpcta gewlvarrmp kptlweskes
      421 eprarcecrp gykritense iselennnll spgscqpptv slakflnenv ksinlsvrei
      481 elavh
//
LOCUS       XP_034195961             482 aa            linear   INV 03-SEP-2025
DEFINITION  serine--tRNA synthetase-like protein Slimp [Osmia lignaria
            lignaria].
ACCESSION   XP_034195961
VERSION     XP_034195961.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340070.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195961.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..482
                     /product="serine--tRNA synthetase-like protein Slimp"
                     /calculated_mol_wt=54590
     Region          58..476
                     /region_name="PLN02320"
                     /note="seryl-tRNA synthetase"
                     /db_xref="CDD:177954"
     CDS             1..482
                     /gene="LOC117611786"
                     /coded_by="XM_034340070.2:1..1449"
                     /db_xref="GeneID:117611786"
ORIGIN      
        1 makylsyfvn iytihnvakh calkhvrkis ncnryyssal lvpgdkrset fvfltpcldf
       61 dniventekl qqelsgrgln inaddvkkmw mfyksvnadk iklqaryeel ihkqkkffgk
      121 tnltaeeqie siklkeqtkv vkndlksvks iisdlqetvl lkmlhlpnev drrtpleapv
      181 vlkhvntlne ysdsklrknh veigkelgll eyknpmqyyl cneaalfelg vlsyagkvfs
      241 andmirvaga dfsrsvvveg sglnhedpsd afiiddhcdv nkgssnrmhl vggaslisfl
      301 amhtkqlinp nhlpvsyfst grqytpfrpg sasiglftvc qtsaavafvv vkdknseeyq
      361 tqfeklfntv stlygnicdh yqivmrpase lrpwesmrvs felwssfskq yievghisac
      421 gdyfskrlli ayqtptgrdf psvisgtvls vprllgclle qnpekfiips kirehmpdyt
      481 ss
//
LOCUS       XP_034195967             143 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein 593 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034195967
VERSION     XP_034195967.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340076.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..143
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..143
                     /product="zinc finger protein 593 homolog"
                     /calculated_mol_wt=16674
     Region          5..125
                     /region_name="UFD2"
                     /note="U1-like Zn-finger-containing protein [General
                     function prediction only]; COG5112"
                     /db_xref="CDD:227443"
     CDS             1..143
                     /gene="LOC117611788"
                     /coded_by="XM_034340076.2:52..483"
                     /db_xref="GeneID:117611788"
ORIGIN      
        1 mtykrrkyhr gdthlkkgwr trrktkdlde idedlkdenv krllnqevdl dkpgagqyyc
       61 ihcaryfind talkdhlttk vhkrrlkale lepytiedse kaagkgnyva pekrkietii
      121 rdsfnmdaes eftsnakkak vds
//
LOCUS       XP_076546768            1456 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546768
VERSION     XP_076546768.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690653.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1456
                     /product="ATPase phospholipid transporting 8B isoform X1"
                     /calculated_mol_wt=164449
     Region          220..1238
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(587..589,791,813..815,880,927..929,1054,1057,1060,
                     1080,1083)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1456
                     /gene="ATP8B"
                     /coded_by="XM_076690653.1:2156..6526"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 miredegeeg srwkyvrgea divssarlkc heeeevkdcd eddkvsvact ttksdaedsr
       61 kgsataasvc ieeenggtrr kkkkrrktrk kqqlqlqelp pldvsgdgpq ahtvlnlpss
      121 sdeveavgec skrdsssslg gasrhntlre slltvlgklv iwkgtryrsa taasssssap
      181 pnsppateci grstsffsne terrirannr eynsqfnyan nyiktskysv ltflplnlfe
      241 qfqrlanfyf lcllvlqmip aissltpitt aipligvlml tavkdayddf qrhssdsqvn
      301 nrksqtlrgt slreekwsqv qvgdvirmen dqfvaadvll lstsepnglc yietaeldge
      361 tnlkcrqcla etaemmdnhe ligqfdgeiv cetpnnllnk fdgnltwkgr kypldndkii
      421 lrgcvlrntq wcygvvifag kdtklmqnsg ktkfkrtsid rllnlliigi vffllsmclf
      481 cmigcgiwes lvgryfqvyl pwdslvpsep mggatviall vffsyaivln tvvpislyvs
      541 vevirfvqsf linwdeemyy aptnthakar tttlneelgq ieyifsdktg tltqnimtfn
      601 kcsiagkcyg dvidevtgev vdltetdkaa rtptmrwkng qefvqvytpi sgpnvrlleq
      661 vdrisniird pgvygspmip hkrsttpsld fsfnkdyepe fkfydsalle avkrdnedvh
      721 sffrllalch tvmpeekngr leyqaqspde aalvsaarnf gfvfkerspn sitievmgkr
      781 evyellcild fnnvrkrmsv ilrkdghlrl yckgadnviy erlkkgseei makslehlnk
      841 fageglrtlc lsvrdldeqf fndwkqrhqe aalsqenrdd kldaiyeeie kdmtllgata
      901 iedklqdgvp qtianlglag ikiwvltgdk qetainigys cqlltddltd vfivdattyd
      961 gvenqltryl etikttsshh nrptlsvvtf rwdkessdte ynpsrdeqde hemeqatgfa
     1021 vvinghslvh alhpqleqlf ldvssqckav iccrvtplqk amvvelikkn knavtlaigd
     1081 gandvsmikt ahigvgisgq eglqavlasd ysigqfrfle rlllvhgrws yyrmskflry
     1141 ffyknfaftl chiwfaffcg fsaqtvfdpm yisvynlfyt slpvmavgif dqdvndknsl
     1201 mypklyapgl qnllfnkkef cwsaihgffa scvlflvpyg tykdgvspkg yvlsdhmllg
     1261 svvatilviv vtvqialdts ywtivnhimv wgsliwyfvl dyfynfvigg syvgsltmam
     1321 seatfwftav isciilvipv lswrfffidv rptlsdrvrl kqrlaqlrsr qsqdilrtps
     1381 trrtrrslrs gyafahqegf grlitsgkim rklpngadfk fampftnnts kqvsvattsp
     1441 kdnasknsht ldtinl
//
LOCUS       XP_076546769            1456 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546769
VERSION     XP_076546769.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690654.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1456
                     /product="ATPase phospholipid transporting 8B isoform X1"
                     /calculated_mol_wt=164449
     Region          220..1238
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(587..589,791,813..815,880,927..929,1054,1057,1060,
                     1080,1083)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1456
                     /gene="ATP8B"
                     /coded_by="XM_076690654.1:1360..5730"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 miredegeeg srwkyvrgea divssarlkc heeeevkdcd eddkvsvact ttksdaedsr
       61 kgsataasvc ieeenggtrr kkkkrrktrk kqqlqlqelp pldvsgdgpq ahtvlnlpss
      121 sdeveavgec skrdsssslg gasrhntlre slltvlgklv iwkgtryrsa taasssssap
      181 pnsppateci grstsffsne terrirannr eynsqfnyan nyiktskysv ltflplnlfe
      241 qfqrlanfyf lcllvlqmip aissltpitt aipligvlml tavkdayddf qrhssdsqvn
      301 nrksqtlrgt slreekwsqv qvgdvirmen dqfvaadvll lstsepnglc yietaeldge
      361 tnlkcrqcla etaemmdnhe ligqfdgeiv cetpnnllnk fdgnltwkgr kypldndkii
      421 lrgcvlrntq wcygvvifag kdtklmqnsg ktkfkrtsid rllnlliigi vffllsmclf
      481 cmigcgiwes lvgryfqvyl pwdslvpsep mggatviall vffsyaivln tvvpislyvs
      541 vevirfvqsf linwdeemyy aptnthakar tttlneelgq ieyifsdktg tltqnimtfn
      601 kcsiagkcyg dvidevtgev vdltetdkaa rtptmrwkng qefvqvytpi sgpnvrlleq
      661 vdrisniird pgvygspmip hkrsttpsld fsfnkdyepe fkfydsalle avkrdnedvh
      721 sffrllalch tvmpeekngr leyqaqspde aalvsaarnf gfvfkerspn sitievmgkr
      781 evyellcild fnnvrkrmsv ilrkdghlrl yckgadnviy erlkkgseei makslehlnk
      841 fageglrtlc lsvrdldeqf fndwkqrhqe aalsqenrdd kldaiyeeie kdmtllgata
      901 iedklqdgvp qtianlglag ikiwvltgdk qetainigys cqlltddltd vfivdattyd
      961 gvenqltryl etikttsshh nrptlsvvtf rwdkessdte ynpsrdeqde hemeqatgfa
     1021 vvinghslvh alhpqleqlf ldvssqckav iccrvtplqk amvvelikkn knavtlaigd
     1081 gandvsmikt ahigvgisgq eglqavlasd ysigqfrfle rlllvhgrws yyrmskflry
     1141 ffyknfaftl chiwfaffcg fsaqtvfdpm yisvynlfyt slpvmavgif dqdvndknsl
     1201 mypklyapgl qnllfnkkef cwsaihgffa scvlflvpyg tykdgvspkg yvlsdhmllg
     1261 svvatilviv vtvqialdts ywtivnhimv wgsliwyfvl dyfynfvigg syvgsltmam
     1321 seatfwftav isciilvipv lswrfffidv rptlsdrvrl kqrlaqlrsr qsqdilrtps
     1381 trrtrrslrs gyafahqegf grlitsgkim rklpngadfk fampftnnts kqvsvattsp
     1441 kdnasknsht ldtinl
//
LOCUS       XP_034196169            1456 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196169
VERSION     XP_034196169.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340278.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1456
                     /product="ATPase phospholipid transporting 8B isoform X1"
                     /calculated_mol_wt=164449
     Region          220..1238
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(587..589,791,813..815,880,927..929,1054,1057,1060,
                     1080,1083)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1456
                     /gene="ATP8B"
                     /coded_by="XM_034340278.2:1737..6107"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 miredegeeg srwkyvrgea divssarlkc heeeevkdcd eddkvsvact ttksdaedsr
       61 kgsataasvc ieeenggtrr kkkkrrktrk kqqlqlqelp pldvsgdgpq ahtvlnlpss
      121 sdeveavgec skrdsssslg gasrhntlre slltvlgklv iwkgtryrsa taasssssap
      181 pnsppateci grstsffsne terrirannr eynsqfnyan nyiktskysv ltflplnlfe
      241 qfqrlanfyf lcllvlqmip aissltpitt aipligvlml tavkdayddf qrhssdsqvn
      301 nrksqtlrgt slreekwsqv qvgdvirmen dqfvaadvll lstsepnglc yietaeldge
      361 tnlkcrqcla etaemmdnhe ligqfdgeiv cetpnnllnk fdgnltwkgr kypldndkii
      421 lrgcvlrntq wcygvvifag kdtklmqnsg ktkfkrtsid rllnlliigi vffllsmclf
      481 cmigcgiwes lvgryfqvyl pwdslvpsep mggatviall vffsyaivln tvvpislyvs
      541 vevirfvqsf linwdeemyy aptnthakar tttlneelgq ieyifsdktg tltqnimtfn
      601 kcsiagkcyg dvidevtgev vdltetdkaa rtptmrwkng qefvqvytpi sgpnvrlleq
      661 vdrisniird pgvygspmip hkrsttpsld fsfnkdyepe fkfydsalle avkrdnedvh
      721 sffrllalch tvmpeekngr leyqaqspde aalvsaarnf gfvfkerspn sitievmgkr
      781 evyellcild fnnvrkrmsv ilrkdghlrl yckgadnviy erlkkgseei makslehlnk
      841 fageglrtlc lsvrdldeqf fndwkqrhqe aalsqenrdd kldaiyeeie kdmtllgata
      901 iedklqdgvp qtianlglag ikiwvltgdk qetainigys cqlltddltd vfivdattyd
      961 gvenqltryl etikttsshh nrptlsvvtf rwdkessdte ynpsrdeqde hemeqatgfa
     1021 vvinghslvh alhpqleqlf ldvssqckav iccrvtplqk amvvelikkn knavtlaigd
     1081 gandvsmikt ahigvgisgq eglqavlasd ysigqfrfle rlllvhgrws yyrmskflry
     1141 ffyknfaftl chiwfaffcg fsaqtvfdpm yisvynlfyt slpvmavgif dqdvndknsl
     1201 mypklyapgl qnllfnkkef cwsaihgffa scvlflvpyg tykdgvspkg yvlsdhmllg
     1261 svvatilviv vtvqialdts ywtivnhimv wgsliwyfvl dyfynfvigg syvgsltmam
     1321 seatfwftav isciilvipv lswrfffidv rptlsdrvrl kqrlaqlrsr qsqdilrtps
     1381 trrtrrslrs gyafahqegf grlitsgkim rklpngadfk fampftnnts kqvsvattsp
     1441 kdnasknsht ldtinl
//
LOCUS       XP_076546770            1455 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546770
VERSION     XP_076546770.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690655.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1455
                     /product="ATPase phospholipid transporting 8B isoform X2"
                     /calculated_mol_wt=164362
     Region          220..1237
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(587..589,791,813..815,880,927..929,1053,1056,1059,
                     1079,1082)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1455
                     /gene="ATP8B"
                     /coded_by="XM_076690655.1:2156..6523"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 miredegeeg srwkyvrgea divssarlkc heeeevkdcd eddkvsvact ttksdaedsr
       61 kgsataasvc ieeenggtrr kkkkrrktrk kqqlqlqelp pldvsgdgpq ahtvlnlpss
      121 sdeveavgec skrdsssslg gasrhntlre slltvlgklv iwkgtryrsa taasssssap
      181 pnsppateci grstsffsne terrirannr eynsqfnyan nyiktskysv ltflplnlfe
      241 qfqrlanfyf lcllvlqmip aissltpitt aipligvlml tavkdayddf qrhssdsqvn
      301 nrksqtlrgt slreekwsqv qvgdvirmen dqfvaadvll lstsepnglc yietaeldge
      361 tnlkcrqcla etaemmdnhe ligqfdgeiv cetpnnllnk fdgnltwkgr kypldndkii
      421 lrgcvlrntq wcygvvifag kdtklmqnsg ktkfkrtsid rllnlliigi vffllsmclf
      481 cmigcgiwes lvgryfqvyl pwdslvpsep mggatviall vffsyaivln tvvpislyvs
      541 vevirfvqsf linwdeemyy aptnthakar tttlneelgq ieyifsdktg tltqnimtfn
      601 kcsiagkcyg dvidevtgev vdltetdkaa rtptmrwkng qefvqvytpi sgpnvrlleq
      661 vdrisniird pgvygspmip hkrsttpsld fsfnkdyepe fkfydsalle avkrdnedvh
      721 sffrllalch tvmpeekngr leyqaqspde aalvsaarnf gfvfkerspn sitievmgkr
      781 evyellcild fnnvrkrmsv ilrkdghlrl yckgadnviy erlkkgseei makslehlnk
      841 fageglrtlc lsvrdldeqf fndwkqrhqe aalsqenrdd kldaiyeeie kdmtllgata
      901 iedklqdgvp qtianlglag ikiwvltgdk qetainigys cqlltddltd vfivdattyd
      961 gvenqltryl etikttsshh nrptlsvvtf rwdkesdtey npsrdeqdeh emeqatgfav
     1021 vinghslvha lhpqleqlfl dvssqckavi ccrvtplqka mvvelikknk navtlaigdg
     1081 andvsmikta higvgisgqe glqavlasdy sigqfrfler lllvhgrwsy yrmskflryf
     1141 fyknfaftlc hiwfaffcgf saqtvfdpmy isvynlfyts lpvmavgifd qdvndknslm
     1201 ypklyapglq nllfnkkefc wsaihgffas cvlflvpygt ykdgvspkgy vlsdhmllgs
     1261 vvatilvivv tvqialdtsy wtivnhimvw gsliwyfvld yfynfviggs yvgsltmams
     1321 eatfwftavi sciilvipvl swrfffidvr ptlsdrvrlk qrlaqlrsrq sqdilrtpst
     1381 rrtrrslrsg yafahqegfg rlitsgkimr klpngadfkf ampftnntsk qvsvattspk
     1441 dnasknshtl dtinl
//
LOCUS       XP_076546771            1451 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546771
VERSION     XP_076546771.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690656.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1451
                     /product="ATPase phospholipid transporting 8B isoform X3"
                     /calculated_mol_wt=163734
     Region          220..1233
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(587..589,791,813..815,880,927..929,1049,1052,1055,
                     1075,1078)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1451
                     /gene="ATP8B"
                     /coded_by="XM_076690656.1:2155..6510"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 miredegeeg srwkyvrgea divssarlkc heeeevkdcd eddkvsvact ttksdaedsr
       61 kgsataasvc ieeenggtrr kkkkrrktrk kqqlqlqelp pldvsgdgpq ahtvlnlpss
      121 sdeveavgec skrdsssslg gasrhntlre slltvlgklv iwkgtryrsa taasssssap
      181 pnsppateci grstsffsne terrirannr eynsqfnyan nyiktskysv ltflplnlfe
      241 qfqrlanfyf lcllvlqmip aissltpitt aipligvlml tavkdayddf qrhssdsqvn
      301 nrksqtlrgt slreekwsqv qvgdvirmen dqfvaadvll lstsepnglc yietaeldge
      361 tnlkcrqcla etaemmdnhe ligqfdgeiv cetpnnllnk fdgnltwkgr kypldndkii
      421 lrgcvlrntq wcygvvifag kdtklmqnsg ktkfkrtsid rllnlliigi vffllsmclf
      481 cmigcgiwes lvgryfqvyl pwdslvpsep mggatviall vffsyaivln tvvpislyvs
      541 vevirfvqsf linwdeemyy aptnthakar tttlneelgq ieyifsdktg tltqnimtfn
      601 kcsiagkcyg dvidevtgev vdltetdkaa rtptmrwkng qefvqvytpi sgpnvrlleq
      661 vdrisniird pgvygspmip hkrsttpsld fsfnkdyepe fkfydsalle avkrdnedvh
      721 sffrllalch tvmpeekngr leyqaqspde aalvsaarnf gfvfkerspn sitievmgkr
      781 evyellcild fnnvrkrmsv ilrkdghlrl yckgadnviy erlkkgseei makslehlnk
      841 fageglrtlc lsvrdldeqf fndwkqrhqe aalsqenrdd kldaiyeeie kdmtllgata
      901 iedklqdgvp qtianlglag ikiwvltgdk qetainigys cqlltddltd vfivdattyd
      961 gvenqltryl etikttsshh nrptlsvvtf ssdteynpsr deqdehemeq atgfavving
     1021 hslvhalhpq leqlfldvss qckaviccrv tplqkamvve likknknavt laigdgandv
     1081 smiktahigv gisgqeglqa vlasdysigq frflerlllv hgrwsyyrms kflryffykn
     1141 faftlchiwf affcgfsaqt vfdpmyisvy nlfytslpvm avgifdqdvn dknslmypkl
     1201 yapglqnllf nkkefcwsai hgffascvlf lvpygtykdg vspkgyvlsd hmllgsvvat
     1261 ilvivvtvqi aldtsywtiv nhimvwgsli wyfvldyfyn fviggsyvgs ltmamseatf
     1321 wftaviscii lvipvlswrf ffidvrptls drvrlkqrla qlrsrqsqdi lrtpstrrtr
     1381 rslrsgyafa hqegfgrlit sgkimrklpn gadfkfampf tnntskqvsv attspkdnas
     1441 knshtldtin l
//
LOCUS       XP_076546772            1450 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546772
VERSION     XP_076546772.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690657.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1450
                     /product="ATPase phospholipid transporting 8B isoform X4"
                     /calculated_mol_wt=163647
     Region          220..1232
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(587..589,791,813..815,880,927..929,1048,1051,1054,
                     1074,1077)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1450
                     /gene="ATP8B"
                     /coded_by="XM_076690657.1:2156..6508"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 miredegeeg srwkyvrgea divssarlkc heeeevkdcd eddkvsvact ttksdaedsr
       61 kgsataasvc ieeenggtrr kkkkrrktrk kqqlqlqelp pldvsgdgpq ahtvlnlpss
      121 sdeveavgec skrdsssslg gasrhntlre slltvlgklv iwkgtryrsa taasssssap
      181 pnsppateci grstsffsne terrirannr eynsqfnyan nyiktskysv ltflplnlfe
      241 qfqrlanfyf lcllvlqmip aissltpitt aipligvlml tavkdayddf qrhssdsqvn
      301 nrksqtlrgt slreekwsqv qvgdvirmen dqfvaadvll lstsepnglc yietaeldge
      361 tnlkcrqcla etaemmdnhe ligqfdgeiv cetpnnllnk fdgnltwkgr kypldndkii
      421 lrgcvlrntq wcygvvifag kdtklmqnsg ktkfkrtsid rllnlliigi vffllsmclf
      481 cmigcgiwes lvgryfqvyl pwdslvpsep mggatviall vffsyaivln tvvpislyvs
      541 vevirfvqsf linwdeemyy aptnthakar tttlneelgq ieyifsdktg tltqnimtfn
      601 kcsiagkcyg dvidevtgev vdltetdkaa rtptmrwkng qefvqvytpi sgpnvrlleq
      661 vdrisniird pgvygspmip hkrsttpsld fsfnkdyepe fkfydsalle avkrdnedvh
      721 sffrllalch tvmpeekngr leyqaqspde aalvsaarnf gfvfkerspn sitievmgkr
      781 evyellcild fnnvrkrmsv ilrkdghlrl yckgadnviy erlkkgseei makslehlnk
      841 fageglrtlc lsvrdldeqf fndwkqrhqe aalsqenrdd kldaiyeeie kdmtllgata
      901 iedklqdgvp qtianlglag ikiwvltgdk qetainigys cqlltddltd vfivdattyd
      961 gvenqltryl etikttsshh nrptlsvvtf sdteynpsrd eqdehemeqa tgfavvingh
     1021 slvhalhpql eqlfldvssq ckaviccrvt plqkamvvel ikknknavtl aigdgandvs
     1081 miktahigvg isgqeglqav lasdysigqf rflerlllvh grwsyyrmsk flryffyknf
     1141 aftlchiwfa ffcgfsaqtv fdpmyisvyn lfytslpvma vgifdqdvnd knslmypkly
     1201 apglqnllfn kkefcwsaih gffascvlfl vpygtykdgv spkgyvlsdh mllgsvvati
     1261 lvivvtvqia ldtsywtivn himvwgsliw yfvldyfynf viggsyvgsl tmamseatfw
     1321 ftavisciil vipvlswrff fidvrptlsd rvrlkqrlaq lrsrqsqdil rtpstrrtrr
     1381 slrsgyafah qegfgrlits gkimrklpng adfkfampft nntskqvsva ttspkdnask
     1441 nshtldtinl
//
LOCUS       XP_076546773            1435 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546773
VERSION     XP_076546773.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690658.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1435
                     /product="ATPase phospholipid transporting 8B isoform X5"
                     /calculated_mol_wt=161964
     Region          199..1217
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(566..568,770,792..794,859,906..908,1033,1036,1039,
                     1059,1062)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1435
                     /gene="ATP8B"
                     /coded_by="XM_076690658.1:2303..6610"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 msvevdtlel evfevkdcde ddkvsvactt tksdaedsrk gsataasvci eeenggtrrk
       61 kkkrrktrkk qqlqlqelpp ldvsgdgpqa htvlnlpsss deveavgecs krdsssslgg
      121 asrhntlres lltvlgklvi wkgtryrsat aasssssapp nsppatecig rstsffsnet
      181 errirannre ynsqfnyann yiktskysvl tflplnlfeq fqrlanfyfl cllvlqmipa
      241 issltpitta ipligvlmlt avkdayddfq rhssdsqvnn rksqtlrgts lreekwsqvq
      301 vgdvirmend qfvaadvlll stsepnglcy ietaeldget nlkcrqclae taemmdnhel
      361 igqfdgeivc etpnnllnkf dgnltwkgrk ypldndkiil rgcvlrntqw cygvvifagk
      421 dtklmqnsgk tkfkrtsidr llnlliigiv ffllsmclfc migcgiwesl vgryfqvylp
      481 wdslvpsepm ggatviallv ffsyaivlnt vvpislyvsv evirfvqsfl inwdeemyya
      541 ptnthakart ttlneelgqi eyifsdktgt ltqnimtfnk csiagkcygd videvtgevv
      601 dltetdkaar tptmrwkngq efvqvytpis gpnvrlleqv drisniirdp gvygspmiph
      661 krsttpsldf sfnkdyepef kfydsallea vkrdnedvhs ffrllalcht vmpeekngrl
      721 eyqaqspdea alvsaarnfg fvfkerspns itievmgkre vyellcildf nnvrkrmsvi
      781 lrkdghlrly ckgadnviye rlkkgseeim akslehlnkf ageglrtlcl svrdldeqff
      841 ndwkqrhqea alsqenrddk ldaiyeeiek dmtllgatai edklqdgvpq tianlglagi
      901 kiwvltgdkq etainigysc qlltddltdv fivdattydg venqltryle tikttsshhn
      961 rptlsvvtfr wdkessdtey npsrdeqdeh emeqatgfav vinghslvha lhpqleqlfl
     1021 dvssqckavi ccrvtplqka mvvelikknk navtlaigdg andvsmikta higvgisgqe
     1081 glqavlasdy sigqfrfler lllvhgrwsy yrmskflryf fyknfaftlc hiwfaffcgf
     1141 saqtvfdpmy isvynlfyts lpvmavgifd qdvndknslm ypklyapglq nllfnkkefc
     1201 wsaihgffas cvlflvpygt ykdgvspkgy vlsdhmllgs vvatilvivv tvqialdtsy
     1261 wtivnhimvw gsliwyfvld yfynfviggs yvgsltmams eatfwftavi sciilvipvl
     1321 swrfffidvr ptlsdrvrlk qrlaqlrsrq sqdilrtpst rrtrrslrsg yafahqegfg
     1381 rlitsgkimr klpngadfkf ampftnntsk qvsvattspk dnasknshtl dtinl
//
LOCUS       XP_076546774            1397 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546774
VERSION     XP_076546774.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690659.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1397
                     /product="ATPase phospholipid transporting 8B isoform X6"
                     /calculated_mol_wt=157744
     Region          220..1179
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(587..589,732,754..756,821,868..870,995,998,1001,
                     1021,1024)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1397
                     /gene="ATP8B"
                     /coded_by="XM_076690659.1:2156..6349"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 miredegeeg srwkyvrgea divssarlkc heeeevkdcd eddkvsvact ttksdaedsr
       61 kgsataasvc ieeenggtrr kkkkrrktrk kqqlqlqelp pldvsgdgpq ahtvlnlpss
      121 sdeveavgec skrdsssslg gasrhntlre slltvlgklv iwkgtryrsa taasssssap
      181 pnsppateci grstsffsne terrirannr eynsqfnyan nyiktskysv ltflplnlfe
      241 qfqrlanfyf lcllvlqmip aissltpitt aipligvlml tavkdayddf qrhssdsqvn
      301 nrksqtlrgt slreekwsqv qvgdvirmen dqfvaadvll lstsepnglc yietaeldge
      361 tnlkcrqcla etaemmdnhe ligqfdgeiv cetpnnllnk fdgnltwkgr kypldndkii
      421 lrgcvlrntq wcygvvifag kdtklmqnsg ktkfkrtsid rllnlliigi vffllsmclf
      481 cmigcgiwes lvgryfqvyl pwdslvpsep mggatviall vffsyaivln tvvpislyvs
      541 vevirfvqsf linwdeemyy aptnthakar tttlneelgq ieyifsdktg tltqnimtfn
      601 kcsiagkcyg dvidevtgev vdltettpsl dfsfnkdyep efkfydsall eavkrdnedv
      661 hsffrllalc htvmpeekng rleyqaqspd eaalvsaarn fgfvfkersp nsitievmgk
      721 revyellcil dfnnvrkrms vilrkdghlr lyckgadnvi yerlkkgsee imakslehln
      781 kfageglrtl clsvrdldeq ffndwkqrhq eaalsqenrd dkldaiyeei ekdmtllgat
      841 aiedklqdgv pqtianlgla gikiwvltgd kqetainigy scqlltddlt dvfivdatty
      901 dgvenqltry letikttssh hnrptlsvvt frwdkessdt eynpsrdeqd ehemeqatgf
      961 avvinghslv halhpqleql fldvssqcka viccrvtplq kamvvelikk nknavtlaig
     1021 dgandvsmik tahigvgisg qeglqavlas dysigqfrfl erlllvhgrw syyrmskflr
     1081 yffyknfaft lchiwfaffc gfsaqtvfdp myisvynlfy tslpvmavgi fdqdvndkns
     1141 lmypklyapg lqnllfnkke fcwsaihgff ascvlflvpy gtykdgvspk gyvlsdhmll
     1201 gsvvatilvi vvtvqialdt sywtivnhim vwgsliwyfv ldyfynfvig gsyvgsltma
     1261 mseatfwfta visciilvip vlswrfffid vrptlsdrvr lkqrlaqlrs rqsqdilrtp
     1321 strrtrrslr sgyafahqeg fgrlitsgki mrklpngadf kfampftnnt skqvsvatts
     1381 pkdnasknsh tldtinl
//
LOCUS       XP_034196175            1268 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196175
VERSION     XP_034196175.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340284.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1268
                     /product="ATPase phospholipid transporting 8B isoform X7"
                     /calculated_mol_wt=144079
     Region          32..1050
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(399..401,603,625..627,692,739..741,866,869,872,892,
                     895)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1268
                     /gene="ATP8B"
                     /coded_by="XM_034340284.2:1754..5560"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 msrsaatrpm qeterriran nreynsqfny annyiktsky svltflplnl feqfqrlanf
       61 yflcllvlqm ipaissltpi ttaipligvl mltavkdayd dfqrhssdsq vnnrksqtlr
      121 gtslreekws qvqvgdvirm endqfvaadv lllstsepng lcyietaeld getnlkcrqc
      181 laetaemmdn heligqfdge ivcetpnnll nkfdgnltwk grkypldndk iilrgcvlrn
      241 tqwcygvvif agkdtklmqn sgktkfkrts idrllnllii givffllsmc lfcmigcgiw
      301 eslvgryfqv ylpwdslvps epmggatvia llvffsyaiv lntvvpisly vsvevirfvq
      361 sflinwdeem yyaptnthak artttlneel gqieyifsdk tgtltqnimt fnkcsiagkc
      421 ygdvidevtg evvdltetdk aartptmrwk ngqefvqvyt pisgpnvrll eqvdrisnii
      481 rdpgvygspm iphkrsttps ldfsfnkdye pefkfydsal leavkrdned vhsffrllal
      541 chtvmpeekn grleyqaqsp deaalvsaar nfgfvfkers pnsitievmg krevyellci
      601 ldfnnvrkrm svilrkdghl rlyckgadnv iyerlkkgse eimakslehl nkfageglrt
      661 lclsvrdlde qffndwkqrh qeaalsqenr ddkldaiyee iekdmtllga taiedklqdg
      721 vpqtianlgl agikiwvltg dkqetainig yscqlltddl tdvfivdatt ydgvenqltr
      781 yletikttss hhnrptlsvv tfrwdkessd teynpsrdeq dehemeqatg favvinghsl
      841 vhalhpqleq lfldvssqck aviccrvtpl qkamvvelik knknavtlai gdgandvsmi
      901 ktahigvgis gqeglqavla sdysigqfrf lerlllvhgr wsyyrmskfl ryffyknfaf
      961 tlchiwfaff cgfsaqtvfd pmyisvynlf ytslpvmavg ifdqdvndkn slmypklyap
     1021 glqnllfnkk efcwsaihgf fascvlflvp ygtykdgvsp kgyvlsdhml lgsvvatilv
     1081 ivvtvqiald tsywtivnhi mvwgsliwyf vldyfynfvi ggsyvgsltm amseatfwft
     1141 avisciilvi pvlswrfffi dvrptlsdrv rlkqrlaqlr srqsqdilrt pstrrtrrsl
     1201 rsgyafahqe gfgrlitsgk imrklpngad fkfampftnn tskqvsvatt spkdnaskns
     1261 htldtinl
//
LOCUS       XP_034196177            1199 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase phospholipid transporting 8B isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196177
VERSION     XP_034196177.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340286.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1199
                     /product="ATPase phospholipid transporting 8B isoform X8"
                     /calculated_mol_wt=135763
     Region          1..981
                     /region_name="P-type_ATPase_APLT_Dnf-like"
                     /note="Aminophospholipid translocases (APLTs), similar to
                     Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2,
                     -10D, -11B, -11C; cd02073"
                     /db_xref="CDD:319770"
     Site            order(330..332,534,556..558,623,670..672,797,800,803,823,
                     826)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319770"
     CDS             1..1199
                     /gene="ATP8B"
                     /coded_by="XM_034340286.2:2828..6427"
                     /db_xref="GeneID:117611871"
ORIGIN      
        1 mipaissltp ittaipligv lmltavkday ddfqrhssds qvnnrksqtl rgtslreekw
       61 sqvqvgdvir mendqfvaad vlllstsepn glcyietael dgetnlkcrq claetaemmd
      121 nheligqfdg eivcetpnnl lnkfdgnltw kgrkypldnd kiilrgcvlr ntqwcygvvi
      181 fagkdtklmq nsgktkfkrt sidrllnlli igivffllsm clfcmigcgi weslvgryfq
      241 vylpwdslvp sepmggatvi allvffsyai vlntvvpisl yvsvevirfv qsflinwdee
      301 myyaptntha kartttlnee lgqieyifsd ktgtltqnim tfnkcsiagk cygdvidevt
      361 gevvdltetd kaartptmrw kngqefvqvy tpisgpnvrl leqvdrisni irdpgvygsp
      421 miphkrsttp sldfsfnkdy epefkfydsa lleavkrdne dvhsffrlla lchtvmpeek
      481 ngrleyqaqs pdeaalvsaa rnfgfvfker spnsitievm gkrevyellc ildfnnvrkr
      541 msvilrkdgh lrlyckgadn viyerlkkgs eeimaksleh lnkfageglr tlclsvrdld
      601 eqffndwkqr hqeaalsqen rddkldaiye eiekdmtllg ataiedklqd gvpqtianlg
      661 lagikiwvlt gdkqetaini gyscqlltdd ltdvfivdat tydgvenqlt ryletiktts
      721 shhnrptlsv vtfrwdkess dteynpsrde qdehemeqat gfavvinghs lvhalhpqle
      781 qlfldvssqc kaviccrvtp lqkamvveli kknknavtla igdgandvsm iktahigvgi
      841 sgqeglqavl asdysigqfr flerlllvhg rwsyyrmskf lryffyknfa ftlchiwfaf
      901 fcgfsaqtvf dpmyisvynl fytslpvmav gifdqdvndk nslmypklya pglqnllfnk
      961 kefcwsaihg ffascvlflv pygtykdgvs pkgyvlsdhm llgsvvatil vivvtvqial
     1021 dtsywtivnh imvwgsliwy fvldyfynfv iggsyvgslt mamseatfwf tavisciilv
     1081 ipvlswrfff idvrptlsdr vrlkqrlaql rsrqsqdilr tpstrrtrrs lrsgyafahq
     1141 egfgrlitsg kimrklpnga dfkfampftn ntskqvsvat tspkdnaskn shtldtinl
//
LOCUS       XP_034196178             967 aa            linear   INV 03-SEP-2025
DEFINITION  methionyl-tRNA synthetase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034196178
VERSION     XP_034196178.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340287.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196178.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..967
                     /product="methionyl-tRNA synthetase 1"
                     /calculated_mol_wt=109361
     Region          1..62
                     /region_name="GST_N_5"
                     /note="Glutathione S-transferase, N-terminal domain;
                     pfam18485"
                     /db_xref="CDD:436537"
     Region          74..167
                     /region_name="GST_C_family"
                     /note="C-terminal, alpha helical domain of the Glutathione
                     S-transferase family; cl02776"
                     /db_xref="CDD:470672"
     Site            order(92,99,131,138..139,142)
                     /site_type="other"
                     /note="N-terminal domain interface [polypeptide binding]"
                     /db_xref="CDD:198286"
     Site            order(92..93,96..97,100,112)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:198286"
     Site            order(99,102..103,143)
                     /site_type="other"
                     /note="substrate binding pocket (H-site) [chemical
                     binding]"
                     /db_xref="CDD:198286"
     Region          249..>854
                     /region_name="PLN02610"
                     /note="probable methionyl-tRNA synthetase"
                     /db_xref="CDD:215329"
     Region          834..881
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          911..>938
                     /region_name="S15_NS1_EPRS_RNA-bind"
                     /note="S15/NS1/EPRS_RNA-binding domain. This short domain
                     consists of a helix-turn-helix structure, which can bind
                     to several types of RNA. It is found in the ribosomal
                     protein S15, the influenza A viral nonstructural protein
                     (NSA) and in several eukaryotic...; cl00349"
                     /db_xref="CDD:469733"
     CDS             1..967
                     /gene="MetRS"
                     /coded_by="XM_034340287.2:300..3203"
                     /db_xref="GeneID:117611873"
ORIGIN      
        1 miistnegnp nalkliiaak maqqsvtikt vqpkvsflgr lpavelpsgl vlfstsaamq
       61 llvpppkesn alnnkwlewd isklqpaivl ytntgsykta sklclwslle elnrelkekk
      121 ylnkdhndls ladtciwvtv wstvlvtdig kelckeylfl kdwlaniell piiqdsikey
      181 kferglraiv siqaaswfpv isslnspank aqpsdisptk ekeleavsqe emqniasnws
      241 sksypdvkns pypvlpkkge rnilitsalp yvnnvphlgn iigcvlsadi farycrqrny
      301 ntlfisgtde ygtateakal eekttpqaic dkffdihndi yrwfsigfdy fgrtttpeqt
      361 eivqsfflri ksqgyilsei vdqllcescn rfladrfveg tcprckyeda rgdqcdgcgh
      421 lvnatdlisp rckvcsnrpi vkkseqffld lpklesklke wsptvekgws tvakvvakpw
      481 lrdglkprci trdlkwgipv pvkgfenkvf yvwfdapfgy isitkrytke yeqwwkptdv
      541 evdlyqfmak dnvpfhaimf pacllaaneg yillkhlmat eylnyedtkf sksrgigvfg
      601 tdardtgipa dvwrfylayv rpetqdsnfn widlvtknnn ellnnfgnfv nralvfaeky
      661 feskipamtl qeedltllvl aqrelssyih nleqaklrdg lkhvlaiskh gnqymqfqep
      721 wvkikgsdtd kkragtiigi ccnlacllsa llapympnvs rqirtqlgld assygyipdi
      781 itnilptghk igkpsplfkk ieekevetlr qkyagkqetk ndaqknndnd vksleaaiae
      841 qgnkvrelka kqdksiwqpe vqillnlkkk lsdlvsgssk ptessqpknk kpevvfvpeq
      901 nggasvdvas lksaitkqge lvrelkakeq ksvwqpevek llklkkqlad ltgitpaptd
      961 kkskkkk
//
LOCUS       XP_034196180             948 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611874 [Osmia lignaria lignaria].
ACCESSION   XP_034196180
VERSION     XP_034196180.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340289.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196180.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..948
                     /product="uncharacterized protein LOC117611874"
                     /calculated_mol_wt=103913
     Region          26..106
                     /region_name="KH-I_KHDC4_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in KH homology domain-containing protein 4 (KHDC4)
                     and similar proteins; cd22385"
                     /db_xref="CDD:411813"
     Region          137..231
                     /region_name="KH-I_KHDC4_rpt2"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in KH homology domain-containing protein 4 (KHDC4)
                     and similar proteins; cd22386"
                     /db_xref="CDD:411814"
     Site            order(152,154..156,158..162,165..166,169..170,175..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411814"
     Region          351..>565
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..948
                     /gene="LOC117611874"
                     /coded_by="XM_034340289.2:229..3075"
                     /db_xref="GeneID:117611874"
ORIGIN      
        1 makgklrgsd snnkltasna tfkngetyts efeineipps artlltkgyi qdeinsysga
       61 tvstrgrfmt eqeklrcpse rplylyiqgh tkhnvdlaiq kineiikteh qsslnrpsrf
      121 tnappplmsl hsgvtsveki cvgienapeg fdlrgriiga gaanllyirg etganvtlrg
      181 rgsqfidpvl gtespeplhl yiehpkpeal qnakqlavnl iqtmqselqs yiqqqpppvq
      241 sqqvieqsqi qqtqfqtmni gtlgqpnvvt iqhqdiiqhp qssvvtlpat iltatvagap
      301 gsgitvpppg vhipphsgpl vpppqtqeiq tfmppppvgq vqligppstv sqvqyqihpg
      361 qplqiqgiqp gsqaspqpvt qmyvmsqppp qnpaqqnfit sssapvsgav syvytqpnvq
      421 rpstpsqgii eavnvnlqqp ppqapinltq qppppllhlh fpppnfppnq ppppvpqtyq
      481 iqyqqvqapv sqsqtqfvlq pgehivppql qqnheqsqav aqhmlppqfe ghapqfhlqv
      541 pppttqtflv pnaqspqhpq qhplppggei qhqqegpvdq gphpqpvppp qivppppsaa
      601 qmpnsmnilt sippptqapp pqnapwlyqa qqpqqmlqsq gqlqvqmppa niplhnvppp
      661 qvqaqiqyha qhmqyqnghv ppqlhytvqp ppqqfeqkpd speqkshgvk rrfsdvetnp
      721 easpyqcvpl paqrhgtgeg drqqqvlhgp sptatglphg drnkllmppp hpgekrnmdq
      781 kppgmssgne qnamgdsmni hpgggpplpp plppqapwqs hhvrvpwgrp ppmprdgdhq
      841 gmcptlpptn mpppqirprg hiegnrsplq nailehqmnv eynqmlpytt kpppynshpl
      901 mqsicnpppp ppphhqqrsq hppqtaqhyq vpisqtyqpp tscppwmn
//
LOCUS       XP_034196182             948 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611874 [Osmia lignaria lignaria].
ACCESSION   XP_034196182
VERSION     XP_034196182.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340291.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196182.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..948
                     /product="uncharacterized protein LOC117611874"
                     /calculated_mol_wt=103913
     Region          26..106
                     /region_name="KH-I_KHDC4_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in KH homology domain-containing protein 4 (KHDC4)
                     and similar proteins; cd22385"
                     /db_xref="CDD:411813"
     Region          137..231
                     /region_name="KH-I_KHDC4_rpt2"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in KH homology domain-containing protein 4 (KHDC4)
                     and similar proteins; cd22386"
                     /db_xref="CDD:411814"
     Site            order(152,154..156,158..162,165..166,169..170,175..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411814"
     Region          351..>565
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..948
                     /gene="LOC117611874"
                     /coded_by="XM_034340291.2:179..3025"
                     /db_xref="GeneID:117611874"
ORIGIN      
        1 makgklrgsd snnkltasna tfkngetyts efeineipps artlltkgyi qdeinsysga
       61 tvstrgrfmt eqeklrcpse rplylyiqgh tkhnvdlaiq kineiikteh qsslnrpsrf
      121 tnappplmsl hsgvtsveki cvgienapeg fdlrgriiga gaanllyirg etganvtlrg
      181 rgsqfidpvl gtespeplhl yiehpkpeal qnakqlavnl iqtmqselqs yiqqqpppvq
      241 sqqvieqsqi qqtqfqtmni gtlgqpnvvt iqhqdiiqhp qssvvtlpat iltatvagap
      301 gsgitvpppg vhipphsgpl vpppqtqeiq tfmppppvgq vqligppstv sqvqyqihpg
      361 qplqiqgiqp gsqaspqpvt qmyvmsqppp qnpaqqnfit sssapvsgav syvytqpnvq
      421 rpstpsqgii eavnvnlqqp ppqapinltq qppppllhlh fpppnfppnq ppppvpqtyq
      481 iqyqqvqapv sqsqtqfvlq pgehivppql qqnheqsqav aqhmlppqfe ghapqfhlqv
      541 pppttqtflv pnaqspqhpq qhplppggei qhqqegpvdq gphpqpvppp qivppppsaa
      601 qmpnsmnilt sippptqapp pqnapwlyqa qqpqqmlqsq gqlqvqmppa niplhnvppp
      661 qvqaqiqyha qhmqyqnghv ppqlhytvqp ppqqfeqkpd speqkshgvk rrfsdvetnp
      721 easpyqcvpl paqrhgtgeg drqqqvlhgp sptatglphg drnkllmppp hpgekrnmdq
      781 kppgmssgne qnamgdsmni hpgggpplpp plppqapwqs hhvrvpwgrp ppmprdgdhq
      841 gmcptlpptn mpppqirprg hiegnrsplq nailehqmnv eynqmlpytt kpppynshpl
      901 mqsicnpppp ppphhqqrsq hppqtaqhyq vpisqtyqpp tscppwmn
//
LOCUS       XP_034196181             948 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611874 [Osmia lignaria lignaria].
ACCESSION   XP_034196181
VERSION     XP_034196181.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340290.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196181.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..948
                     /product="uncharacterized protein LOC117611874"
                     /calculated_mol_wt=103913
     Region          26..106
                     /region_name="KH-I_KHDC4_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in KH homology domain-containing protein 4 (KHDC4)
                     and similar proteins; cd22385"
                     /db_xref="CDD:411813"
     Region          137..231
                     /region_name="KH-I_KHDC4_rpt2"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in KH homology domain-containing protein 4 (KHDC4)
                     and similar proteins; cd22386"
                     /db_xref="CDD:411814"
     Site            order(152,154..156,158..162,165..166,169..170,175..178)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411814"
     Region          351..>565
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..948
                     /gene="LOC117611874"
                     /coded_by="XM_034340290.2:140..2986"
                     /db_xref="GeneID:117611874"
ORIGIN      
        1 makgklrgsd snnkltasna tfkngetyts efeineipps artlltkgyi qdeinsysga
       61 tvstrgrfmt eqeklrcpse rplylyiqgh tkhnvdlaiq kineiikteh qsslnrpsrf
      121 tnappplmsl hsgvtsveki cvgienapeg fdlrgriiga gaanllyirg etganvtlrg
      181 rgsqfidpvl gtespeplhl yiehpkpeal qnakqlavnl iqtmqselqs yiqqqpppvq
      241 sqqvieqsqi qqtqfqtmni gtlgqpnvvt iqhqdiiqhp qssvvtlpat iltatvagap
      301 gsgitvpppg vhipphsgpl vpppqtqeiq tfmppppvgq vqligppstv sqvqyqihpg
      361 qplqiqgiqp gsqaspqpvt qmyvmsqppp qnpaqqnfit sssapvsgav syvytqpnvq
      421 rpstpsqgii eavnvnlqqp ppqapinltq qppppllhlh fpppnfppnq ppppvpqtyq
      481 iqyqqvqapv sqsqtqfvlq pgehivppql qqnheqsqav aqhmlppqfe ghapqfhlqv
      541 pppttqtflv pnaqspqhpq qhplppggei qhqqegpvdq gphpqpvppp qivppppsaa
      601 qmpnsmnilt sippptqapp pqnapwlyqa qqpqqmlqsq gqlqvqmppa niplhnvppp
      661 qvqaqiqyha qhmqyqnghv ppqlhytvqp ppqqfeqkpd speqkshgvk rrfsdvetnp
      721 easpyqcvpl paqrhgtgeg drqqqvlhgp sptatglphg drnkllmppp hpgekrnmdq
      781 kppgmssgne qnamgdsmni hpgggpplpp plppqapwqs hhvrvpwgrp ppmprdgdhq
      841 gmcptlpptn mpppqirprg hiegnrsplq nailehqmnv eynqmlpytt kpppynshpl
      901 mqsicnpppp ppphhqqrsq hppqtaqhyq vpisqtyqpp tscppwmn
//
LOCUS       XP_034196185             338 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin-dependent kinase 7 [Osmia lignaria lignaria].
ACCESSION   XP_034196185
VERSION     XP_034196185.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340294.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="cyclin-dependent kinase 7"
                     /calculated_mol_wt=37934
     Region          7..304
                     /region_name="STKc_CDK7"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Cyclin-Dependent protein Kinase 7; cd07841"
                     /db_xref="CDD:270833"
     Site            order(14..17,19..20,22,35,37,57,71,87..90,93,95..96,133,
                     135,137..138,140,151,154,156,166,168..171,173,211..212)
                     /site_type="active"
                     /db_xref="CDD:270833"
     Site            order(14,16..20,22,35,37,71,87..90,137..138,156)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270833"
     Site            order(49..51,53,56..57,59..60,63..64,76,78,83,122,
                     125..128,156,158..161,165..166,277,281..283)
                     /site_type="other"
                     /note="CDK/cyclin interface [polypeptide binding]"
                     /db_xref="CDD:270833"
     Site            order(57,95,133,135,154,166,168..171,173,211..212)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270833"
     Site            order(150..161,163..173)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270833"
     CDS             1..338
                     /gene="Cdk7"
                     /coded_by="XM_034340294.2:232..1248"
                     /db_xref="GeneID:117611876"
ORIGIN      
        1 mseklrryek idflgegqfa tvykakdiet gkivavkkik vgsraeakdg inrtalreik
       61 llqelkhdnv iglldvfgyk snvslvfdfm dtdleiiikd snivltaani kaymiqtlqg
      121 ldylhhnwil hrdlkpnnll vdaegvlkig dfglakffgs pnrinthqvv trwyrapell
      181 ygarlygtgi dmwavgcila elllrvpflp gesdldqltr ifqtlgtpte etwpgmtelp
      241 dfiqfkpfpg tplkhiftaa gddlldlias llnvnplerc tcdqalqmpy fsnkpaptpg
      301 prlplptsvk rqpeekpslk rkllesmdga slakrlqf
//
LOCUS       XP_034196186             322 aa            linear   INV 03-SEP-2025
DEFINITION  pancreatic triacylglycerol lipase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196186
VERSION     XP_034196186.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340295.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="pancreatic triacylglycerol lipase isoform X1"
                     /calculated_mol_wt=35513
     Region          46..315
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            159..163
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(161,185,256)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(245..246,253..254)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..322
                     /gene="LOC117611877"
                     /coded_by="XM_034340295.2:416..1384"
                     /db_xref="GeneID:117611877"
ORIGIN      
        1 mspvetllfl aivlvsayay pqntllndve tvirsvfkaa repatfnlyt renpfgeeql
       61 flnntevlya shfnesrptk fiihgfsdtg neawvrglid ayllhedvnv ivvgwgilaa
      121 diyplaannt rrvgeflgdf leflnhesnl eykdvhisgh slgshvagfa gayldgrigr
      181 itgldpaspl fetisgivdp efrldptdaq fvdvihtsgp afgflaplgh adfypnngtf
      241 pqpgcsfipt ttycshsrah qfmtesigst agfkartces wekykegrcd ynpivlmgey
      301 astslrgkfy lstndappfa ie
//
LOCUS       XP_076546775             268 aa            linear   INV 03-SEP-2025
DEFINITION  endothelial lipase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546775
VERSION     XP_076546775.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690660.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="endothelial lipase isoform X2"
                     /calculated_mol_wt=29556
     Region          46..>259
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            159..163
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(245..246,258..259)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..268
                     /gene="LOC117611877"
                     /coded_by="XM_076690660.1:438..1244"
                     /db_xref="GeneID:117611877"
ORIGIN      
        1 mspvetllfl aivlvsayay pqntllndve tvirsvfkaa repatfnlyt renpfgeeql
       61 flnntevlya shfnesrptk fiihgfsdtg neawvrglid ayllhedvnv ivvgwgilaa
      121 diyplaannt rrvgeflgdf leflnhesnl eykdvhisgh slgshvagfa gayldgrigr
      181 itgldpaspl fetisgivdp efrldptdaq fvdvihtsgp afgflaplgh adfypnngtf
      241 pqpgcsfipt tsyeenficq rttplhsr
//
LOCUS       XP_034196183             636 aa            linear   INV 03-SEP-2025
DEFINITION  ionotropic receptor 75a [Osmia lignaria lignaria].
ACCESSION   XP_034196183
VERSION     XP_034196183.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340292.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="ionotropic receptor 75a"
                     /calculated_mol_wt=73976
     CDS             1..636
                     /gene="LOC117611875"
                     /coded_by="XM_034340292.2:1..1911"
                     /db_xref="GeneID:117611875"
ORIGIN      
        1 mivrkmhfrs ffylqlivtl yannvniird yflfkqvngv vgfscgnsen dyqiirifne
       61 agiaasikql tsnidisqfl ysdhwnlgvf ldlqcsftdd dvvklfsets ayymfdhlhq
      121 wlilgesmnr tiqllndsvf siitdvvisv pknddyilyd vynhckpcgg llnitefgsw
      181 rendgldinl kadkfsrrwn yhkmkvrvag vvtqrpkdqi lteylqeqns valtdnwpkf
      241 gyslivdiae ifnftlevle ldhwekndrn gplvtglskr ifdlgyypsi ltnerlnyad
      301 vitqvwpvrt cfmfltvpss kidiniifrp fstnvwymif llsfiiifal wmilkveknv
      361 nnsdcgttvl iivaamcqqg lpfvsnkwag rvaflhimif gllvynyysa aivsarlnap
      421 snkmndslys linskmklta ykdiylnfll rapvpevqyf kkywdiipen kkfrpieegv
      481 kgimnpryay hadpinvypv iermfskqmi cqlseihlfr pntlglwstk nspfreitki
      541 gliriatagi rkrevlrwts rkpycdkdkh yvssvtihet ipiilllfig ivlsaiicfi
      601 enivfhvlqt kqrkenykqv lqkyiavirk kiitkq
//
LOCUS       XP_034196151             418 aa            linear   INV 03-SEP-2025
DEFINITION  RNA-binding protein MEX3B-like [Osmia lignaria lignaria].
ACCESSION   XP_034196151
VERSION     XP_034196151.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340260.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..418
                     /product="RNA-binding protein MEX3B-like"
                     /calculated_mol_wt=43263
     Region          81..153
                     /region_name="KH-I_MEX3_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in the family of MEX-3 RNA-binding proteins;
                     cd22423"
                     /db_xref="CDD:411851"
     Site            order(90..91,93..95,97..101,103..105,108,114..120,
                     148..149,152..153)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411851"
     Region          175..246
                     /region_name="KH-I_MEX3_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in the family of MEX-3 RNA-binding proteins;
                     cd22424"
                     /db_xref="CDD:411852"
     Site            order(187,189..191,193..197,200..201,204,210..216,245)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411852"
     Region          365..417
                     /region_name="RING-HC_MEX3"
                     /note="RING finger, HC subclass, found in RNA-binding
                     proteins of the evolutionarily-conserved MEX-3 family;
                     cd16518"
                     /db_xref="CDD:438181"
     CDS             1..418
                     /gene="LOC143305213"
                     /coded_by="XM_034340260.2:2930..4186"
                     /db_xref="GeneID:143305213"
ORIGIN      
        1 mptslfsemd rgangggtas ledqralqla lelsmlgleg tpgcpgtgtg tgtgtandpd
       61 plqttpagvf eearskksqn mtecvpvpss ehvaeivgrq gckikalrak tntyiktpvr
      121 geepvfvvtg rkedvarakr eilsaaehfs qirasrkssl gallgappgp pasvpghvti
      181 qvrvpyrvvg lvvgpkgati kriqhqthty ivtpsrdkep vfevtglpes veaarreiea
      241 hialrtgtgp slddsellsv lcrgglgsil gcldppgsng sngsngafss sgscssssss
      301 sgapglndiv aiwgagverd eslgespsfe sassiwsfpg valpsrsspp asiptsptds
      361 llgrrecvvc gdkevtaalv pcghnhfcld csnrvcessd pscpvcsrpv lqalrifs
//
LOCUS       XP_034196153             223 aa            linear   INV 03-SEP-2025
DEFINITION  methyl-CpG-binding domain protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196153
VERSION     XP_034196153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340262.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..223
                     /product="methyl-CpG-binding domain protein 2 isoform X1"
                     /calculated_mol_wt=24858
     Region          37..121
                     /region_name="MBDa"
                     /note="p55-binding region of Methyl-CpG-binding domain
                     proteins MBD; pfam16564"
                     /db_xref="CDD:465179"
     Region          125..218
                     /region_name="MBD_C"
                     /note="C-terminal domain of methyl-CpG binding protein 2
                     and 3; pfam14048"
                     /db_xref="CDD:464072"
     CDS             1..223
                     /gene="MBD-like"
                     /coded_by="XM_034340262.2:129..800"
                     /db_xref="GeneID:117611861"
ORIGIN      
        1 mnmsvekkky psalptnwpt reetsrksqn qlsstgkvdv yyysrgvrnd aslvppirqt
       61 asifkqpvti yktqegkvkd ikhgnqekpk qegadktdgk ygsqdkpkql fwekrleglr
      121 acdpdgyefd amdlpkslkp vgpyiteetl lqsvatalhv ssqpvtgqtg sktaleknpg
      181 vflnpdqplv qavsivdedi krqedrvala rkklqealrt ipt
//
LOCUS       XP_076546776             212 aa            linear   INV 03-SEP-2025
DEFINITION  methyl-CpG-binding domain protein 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546776
VERSION     XP_076546776.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690661.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..212
                     /product="methyl-CpG-binding domain protein 2 isoform X2"
                     /calculated_mol_wt=23607
     Region          37..121
                     /region_name="MBDa"
                     /note="p55-binding region of Methyl-CpG-binding domain
                     proteins MBD; pfam16564"
                     /db_xref="CDD:465179"
     Region          125..207
                     /region_name="MBD_C"
                     /note="C-terminal domain of methyl-CpG binding protein 2
                     and 3; pfam14048"
                     /db_xref="CDD:464072"
     CDS             1..212
                     /gene="MBD-like"
                     /coded_by="XM_076690661.1:130..768"
                     /db_xref="GeneID:117611861"
ORIGIN      
        1 mnmsvekkky psalptnwpt reetsrksqn qlsstgkvdv yyysrgvrnd aslvppirqt
       61 asifkqpvti yktqegkvkd ikhgnqekpk qegadktdgk ygsqdkpkql fwekrleglr
      121 acdpdgyefd amdlpkslkp vgpyiteetl lqsvatalhv ssqpvtgqtg sktaleknpg
      181 avsivdedik rqedrvalar kklqealrti pt
//
LOCUS       XP_034196154             205 aa            linear   INV 03-SEP-2025
DEFINITION  methyl-CpG-binding domain protein 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196154
VERSION     XP_034196154.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340263.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..205
                     /product="methyl-CpG-binding domain protein 2 isoform X3"
                     /calculated_mol_wt=22924
     Region          37..103
                     /region_name="MBDa"
                     /note="p55-binding region of Methyl-CpG-binding domain
                     proteins MBD; pfam16564"
                     /db_xref="CDD:465179"
     Region          107..200
                     /region_name="MBD_C"
                     /note="C-terminal domain of methyl-CpG binding protein 2
                     and 3; pfam14048"
                     /db_xref="CDD:464072"
     CDS             1..205
                     /gene="MBD-like"
                     /coded_by="XM_034340263.2:127..744"
                     /db_xref="GeneID:117611861"
ORIGIN      
        1 mnmsvekkky psalptnwpt reetsrksqn qlsstgkvdv yyysrgvrnd aslvppirqt
       61 asifkqpvti yktqegkvkd ikhgnqekpk qlfwekrleg lracdpdgye fdamdlpksl
      121 kpvgpyitee tllqsvatal hvssqpvtgq tgsktalekn pgvflnpdqp lvqavsivde
      181 dikrqedrva larkklqeal rtipt
//
LOCUS       XP_076546777             208 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611860 [Osmia lignaria lignaria].
ACCESSION   XP_076546777
VERSION     XP_076546777.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690662.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="uncharacterized protein LOC117611860"
                     /calculated_mol_wt=23361
     CDS             1..208
                     /gene="LOC117611860"
                     /coded_by="XM_076690662.1:459..1085"
                     /db_xref="GeneID:117611860"
ORIGIN      
        1 msfsrdngsf vacsfvgrrs vlcwngcvll vphkrvhgqv ygtsvfrrct kgnnqfrgds
       61 adkhhqvgrg lskealvhrl nswgtfdsql lyvidvdlls lswarqtrav drktghfvvf
      121 prtttkptck hqelynylmk qaqappirhl qwrtmlsvgt spglsnppqp vytqspvsdd
      181 getrgrsalf eiptfylhsv vssrcplk
//
LOCUS       XP_034196220             646 aa            linear   INV 03-SEP-2025
DEFINITION  protein regulator of cytokinesis 1-like [Osmia lignaria lignaria].
ACCESSION   XP_034196220
VERSION     XP_034196220.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340329.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..646
                     /product="protein regulator of cytokinesis 1-like"
                     /calculated_mol_wt=74557
     Region          21..456
                     /region_name="MAP65_ASE1"
                     /note="Microtubule associated protein (MAP65/ASE1 family);
                     pfam03999"
                     /db_xref="CDD:427641"
     CDS             1..646
                     /gene="LOC117611896"
                     /coded_by="XM_034340329.2:137..2077"
                     /db_xref="GeneID:117611896"
ORIGIN      
        1 msdiskwesi vqetivklrs afshlnkiwe digfneetct vyceqasnhi ktllnemvse
       61 seskkdiiln nvkelmeqia vlskelgtdv aingyeelpl keieqvlitd lhklqyckeq
      121 rmvrlkella kerllckslg tqpinieekl pseeevnsfk lylekqeaek nrleamfkem
      181 rravvimmdd lgispstnfe hlvykdsenf vfnssnmskl relrdelkhq vdqakehved
      241 ikhelialwk yldepqdicq sflnsyvgys vatinalase lerckekrrq niakyvaqvr
      301 selarlwdlc kfseqqrkqf ihynsdtyte dlltlhelev krvqefyean ksifellker
      361 dnlwikmkel lqrannpdrf ynrggqllme ekerktiqkk lpkieeqlqs likeyesqhg
      421 evftingvsi eellqesweh lneeketikq arkeakdksv kkvtlsaskk aassstkktp
      481 tvlstrhhtp lssskrkllf taspnatakr rnvsivgsgs klrksgrivk ntrcsgnkns
      541 ekensisqss ldttysqfke hlkdkqelrs sllsekvlfn ankskmrtpv rtpvkpsrkn
      601 lltptgvatg tpskfpqsql hrsprsprta qsstrlavtt plpiif
//
LOCUS       XP_034196221             544 aa            linear   INV 03-SEP-2025
DEFINITION  ADP-ribosylation factor GTPase activating protein 3 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034196221
VERSION     XP_034196221.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340330.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..544
                     /product="ADP-ribosylation factor GTPase activating
                     protein 3 isoform X1"
                     /calculated_mol_wt=60263
     Region          10..125
                     /region_name="ArfGap_ArfGap2_3_like"
                     /note="Arf1 GTPase-activating protein 2/3-like; cd08831"
                     /db_xref="CDD:350060"
     Site            order(27,30,47,50,55)
                     /site_type="other"
                     /note="arginine finger"
                     /db_xref="CDD:350060"
     Site            order(27,30,47,50)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:350060"
     CDS             1..544
                     /gene="ArfGAP3"
                     /coded_by="XM_034340330.2:41..1675"
                     /db_xref="GeneID:117611897"
ORIGIN      
        1 madpapsksd ieeifkrlra iptnktcfdc naknpawssv tygvflcidc savhrglgvh
       61 ltfvrstqld tnwtwlqlrn mqlggnanar kyftqhnctt tdaqqkynsr aamqyrekla
      121 qasaqamrhy gtkvflpstk nktmlhleeg ptstseeqge idffkehenf evhhnessvp
      181 letnsislfn siphndnedk nnqkdmpdmp snflgptvkl sdstsnlipe rkstiggrkv
      241 qakrpglgkk agglgaqrvk tnfdeleksv aeagkelqek dhqentkeee eeiatrlayr
      301 yeqnlseqak kveqrakhld pskatqaerl gmgfntrsga shsavgdmrt itqetsskti
      361 tasepkprei dierdlfisl defyaalstp ynsnnnnksr neevlviaep eppkrtapps
      421 kpnsnkndnk ilegeaqkkf gsakaissdq yfqdskddds werksnlrrf egsssissad
      481 yfgtgsstat sptsslsmrl gggragvdld dvresvrqgv nkvagrlssl anaavsslqd
      541 rygl
//
LOCUS       XP_034196222             533 aa            linear   INV 03-SEP-2025
DEFINITION  ADP-ribosylation factor GTPase activating protein 3 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034196222
VERSION     XP_034196222.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340331.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="ADP-ribosylation factor GTPase activating
                     protein 3 isoform X2"
                     /calculated_mol_wt=59015
     Region          9..472
                     /region_name="PLN03114"
                     /note="ADP-ribosylation factor GTPase-activating protein
                     AGD10; Provisional"
                     /db_xref="CDD:178661"
     Region          10..125
                     /region_name="ArfGap_ArfGap2_3_like"
                     /note="Arf1 GTPase-activating protein 2/3-like; cd08831"
                     /db_xref="CDD:350060"
     Site            order(27,30,47,50,55)
                     /site_type="other"
                     /note="arginine finger"
                     /db_xref="CDD:350060"
     Site            order(27,30,47,50)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:350060"
     CDS             1..533
                     /gene="ArfGAP3"
                     /coded_by="XM_034340331.2:41..1642"
                     /db_xref="GeneID:117611897"
ORIGIN      
        1 madpapsksd ieeifkrlra iptnktcfdc naknpawssv tygvflcidc savhrglgvh
       61 ltfvrstqld tnwtwlqlrn mqlggnanar kyftqhnctt tdaqqkynsr aamqyrekla
      121 qasaqamrhy gtklhleegp tstseeqgei dffkehenfe vhhnessvpl etnsislfns
      181 iphndnedkn nqkdmpdmps nflgptvkls dstsnliper kstiggrkvq akrpglgkka
      241 gglgaqrvkt nfdeleksva eagkelqekd hqentkeeee eiatrlayry eqnlseqakk
      301 veqrakhldp skatqaerlg mgfntrsgas hsavgdmrti tqetssktit asepkpreid
      361 ierdlfisld efyaalstpy nsnnnnksrn eevlviaepe ppkrtappsk pnsnkndnki
      421 legeaqkkfg sakaissdqy fqdskdddsw erksnlrrfe gsssissady fgtgsstats
      481 ptsslsmrlg ggragvdldd vresvrqgvn kvagrlssla naavsslqdr ygl
//
LOCUS       XP_034195171             550 aa            linear   INV 03-SEP-2025
DEFINITION  chaperonin containing TCP1 subunit 3 [Osmia lignaria lignaria].
ACCESSION   XP_034195171
VERSION     XP_034195171.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339280.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..550
                     /product="chaperonin containing TCP1 subunit 3"
                     /calculated_mol_wt=60586
     Region          9..531
                     /region_name="chap_CCT_gamma"
                     /note="T-complex protein 1, gamma subunit; TIGR02344"
                     /db_xref="CDD:274085"
     CDS             1..550
                     /gene="CCT3"
                     /coded_by="XM_034339280.2:138..1790"
                     /db_xref="GeneID:117611342"
ORIGIN      
        1 mfgsgaasiv vlspntkrdt grkvqreniq agkaiadvir tclgpqamlk mlmdpmggiv
       61 mtndgnailr eitvqhpagk smieiartqd eevgdgttsv ivlageilav gepfleqnmh
      121 ptviirafrq aledmvtiln eqisvdldrn drskliqvin scvgtkfigr wselacqial
      181 davntvmlee ngrreidikr yakvekipgg svedstvlkg vmfskdvthp kmrryiknpr
      241 ivlldcsley kkgesqtnie imkdtdftri leleeeyvkk icediiavkp dvvitekgvs
      301 diaqhylvka gisairrlrk sdinriarac gatvvnrtee lreedigtka glfevkklgd
      361 dyfcfiteck dpkactiilr gaskdilnet ernlqdalhv arnlliepkl vpgggavema
      421 vsrlltekag rlagveqwpy kavaqaleii prtlaqncga ntirtltalr akhatggvtw
      481 gidgetgqlv dmkergiwep lsvklqtykt aietailllr iddivsgskk kksdneptpp
      541 aqvseesmke
//
LOCUS       XP_034196224             544 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone-induced protein 78C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196224
VERSION     XP_034196224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340333.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..544
                     /product="ecdysone-induced protein 78C isoform X1"
                     /calculated_mol_wt=58845
     Region          115..195
                     /region_name="NR_DBD_DmE78_like"
                     /note="DNA-binding domain of Drosophila ecdysone-induced
                     protein 78 (E78) like is composed of two C4-type zinc
                     fingers; cd07165"
                     /db_xref="CDD:143539"
     Site            order(115,118,132,135,151,157,167,170)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143539"
     Site            order(125..127,133..134,136,138,141,144,164..165,168,171,
                     182,185)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143539"
     Site            order(162..163,165..166)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:143539"
     Region          336..535
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(342..343,346,349..350,379..380,383..384,387,421)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(351,354,358,363,368..369,371..372,375..376)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..544
                     /gene="Eip78C"
                     /coded_by="XM_034340333.2:2026..3660"
                     /db_xref="GeneID:117611898"
ORIGIN      
        1 mdvfkidqsl saglgsapap dtltpevsfd agsefnlsff dgpeenstqg fsdpgsvgsa
       61 saspppgtpg nipvnpsnaa lpivqvpsgi vikqeqhyaa adsnsstpak sfvacrvcgd
      121 kasgyhygvt scegckgffr rsiqkqieyr clrdgkclvi rlnrnrcqyc rfkkclavgm
      181 srdsvrygrv pkrsrergpe davptttttt attgvqqlts pgggnnnnnn nnnnnttsqn
      241 nnnssgqgqn aipcvpaaav veadqsdadv kqlavydvil qvsqahhahc gfteestrgi
      301 vrkpmiipas tssqpasped peaasstaet vesqriwlwq qfatrvtpsv qrvvefakrv
      361 pgfcdlsqdd qlilikvgff evwlshiskm ttdssmtfed gtcitrqqme lmyepdfvsa
      421 lmqftsslng hqlsdtelgl fsgavllger pglndvkavq rlqdrlleal kvqalrnhtv
      481 sgeatsaggc sgvssvsqri pelralgarh anlldwfrrn wtklklpplf aeifdipkce
      541 edlq
//
LOCUS       XP_034196225             544 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone-induced protein 78C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196225
VERSION     XP_034196225.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340334.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..544
                     /product="ecdysone-induced protein 78C isoform X1"
                     /calculated_mol_wt=58845
     Region          115..195
                     /region_name="NR_DBD_DmE78_like"
                     /note="DNA-binding domain of Drosophila ecdysone-induced
                     protein 78 (E78) like is composed of two C4-type zinc
                     fingers; cd07165"
                     /db_xref="CDD:143539"
     Site            order(115,118,132,135,151,157,167,170)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143539"
     Site            order(125..127,133..134,136,138,141,144,164..165,168,171,
                     182,185)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143539"
     Site            order(162..163,165..166)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:143539"
     Region          336..535
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(342..343,346,349..350,379..380,383..384,387,421)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(351,354,358,363,368..369,371..372,375..376)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..544
                     /gene="Eip78C"
                     /coded_by="XM_034340334.2:4123..5757"
                     /db_xref="GeneID:117611898"
ORIGIN      
        1 mdvfkidqsl saglgsapap dtltpevsfd agsefnlsff dgpeenstqg fsdpgsvgsa
       61 saspppgtpg nipvnpsnaa lpivqvpsgi vikqeqhyaa adsnsstpak sfvacrvcgd
      121 kasgyhygvt scegckgffr rsiqkqieyr clrdgkclvi rlnrnrcqyc rfkkclavgm
      181 srdsvrygrv pkrsrergpe davptttttt attgvqqlts pgggnnnnnn nnnnnttsqn
      241 nnnssgqgqn aipcvpaaav veadqsdadv kqlavydvil qvsqahhahc gfteestrgi
      301 vrkpmiipas tssqpasped peaasstaet vesqriwlwq qfatrvtpsv qrvvefakrv
      361 pgfcdlsqdd qlilikvgff evwlshiskm ttdssmtfed gtcitrqqme lmyepdfvsa
      421 lmqftsslng hqlsdtelgl fsgavllger pglndvkavq rlqdrlleal kvqalrnhtv
      481 sgeatsaggc sgvssvsqri pelralgarh anlldwfrrn wtklklpplf aeifdipkce
      541 edlq
//
LOCUS       XP_034196226             544 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone-induced protein 78C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196226
VERSION     XP_034196226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340335.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..544
                     /product="ecdysone-induced protein 78C isoform X1"
                     /calculated_mol_wt=58845
     Region          115..195
                     /region_name="NR_DBD_DmE78_like"
                     /note="DNA-binding domain of Drosophila ecdysone-induced
                     protein 78 (E78) like is composed of two C4-type zinc
                     fingers; cd07165"
                     /db_xref="CDD:143539"
     Site            order(115,118,132,135,151,157,167,170)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143539"
     Site            order(125..127,133..134,136,138,141,144,164..165,168,171,
                     182,185)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143539"
     Site            order(162..163,165..166)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:143539"
     Region          336..535
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(342..343,346,349..350,379..380,383..384,387,421)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(351,354,358,363,368..369,371..372,375..376)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..544
                     /gene="Eip78C"
                     /coded_by="XM_034340335.2:2142..3776"
                     /db_xref="GeneID:117611898"
ORIGIN      
        1 mdvfkidqsl saglgsapap dtltpevsfd agsefnlsff dgpeenstqg fsdpgsvgsa
       61 saspppgtpg nipvnpsnaa lpivqvpsgi vikqeqhyaa adsnsstpak sfvacrvcgd
      121 kasgyhygvt scegckgffr rsiqkqieyr clrdgkclvi rlnrnrcqyc rfkkclavgm
      181 srdsvrygrv pkrsrergpe davptttttt attgvqqlts pgggnnnnnn nnnnnttsqn
      241 nnnssgqgqn aipcvpaaav veadqsdadv kqlavydvil qvsqahhahc gfteestrgi
      301 vrkpmiipas tssqpasped peaasstaet vesqriwlwq qfatrvtpsv qrvvefakrv
      361 pgfcdlsqdd qlilikvgff evwlshiskm ttdssmtfed gtcitrqqme lmyepdfvsa
      421 lmqftsslng hqlsdtelgl fsgavllger pglndvkavq rlqdrlleal kvqalrnhtv
      481 sgeatsaggc sgvssvsqri pelralgarh anlldwfrrn wtklklpplf aeifdipkce
      541 edlq
//
LOCUS       XP_034196227             544 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone-induced protein 78C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196227
VERSION     XP_034196227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340336.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..544
                     /product="ecdysone-induced protein 78C isoform X1"
                     /calculated_mol_wt=58845
     Region          115..195
                     /region_name="NR_DBD_DmE78_like"
                     /note="DNA-binding domain of Drosophila ecdysone-induced
                     protein 78 (E78) like is composed of two C4-type zinc
                     fingers; cd07165"
                     /db_xref="CDD:143539"
     Site            order(115,118,132,135,151,157,167,170)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143539"
     Site            order(125..127,133..134,136,138,141,144,164..165,168,171,
                     182,185)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143539"
     Site            order(162..163,165..166)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:143539"
     Region          336..535
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(342..343,346,349..350,379..380,383..384,387,421)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(351,354,358,363,368..369,371..372,375..376)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..544
                     /gene="Eip78C"
                     /coded_by="XM_034340336.2:1812..3446"
                     /db_xref="GeneID:117611898"
ORIGIN      
        1 mdvfkidqsl saglgsapap dtltpevsfd agsefnlsff dgpeenstqg fsdpgsvgsa
       61 saspppgtpg nipvnpsnaa lpivqvpsgi vikqeqhyaa adsnsstpak sfvacrvcgd
      121 kasgyhygvt scegckgffr rsiqkqieyr clrdgkclvi rlnrnrcqyc rfkkclavgm
      181 srdsvrygrv pkrsrergpe davptttttt attgvqqlts pgggnnnnnn nnnnnttsqn
      241 nnnssgqgqn aipcvpaaav veadqsdadv kqlavydvil qvsqahhahc gfteestrgi
      301 vrkpmiipas tssqpasped peaasstaet vesqriwlwq qfatrvtpsv qrvvefakrv
      361 pgfcdlsqdd qlilikvgff evwlshiskm ttdssmtfed gtcitrqqme lmyepdfvsa
      421 lmqftsslng hqlsdtelgl fsgavllger pglndvkavq rlqdrlleal kvqalrnhtv
      481 sgeatsaggc sgvssvsqri pelralgarh anlldwfrrn wtklklpplf aeifdipkce
      541 edlq
//
LOCUS       XP_034196228             538 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone-induced protein 78C isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196228
VERSION     XP_034196228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340337.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..538
                     /product="ecdysone-induced protein 78C isoform X2"
                     /calculated_mol_wt=58284
     Region          115..195
                     /region_name="NR_DBD_DmE78_like"
                     /note="DNA-binding domain of Drosophila ecdysone-induced
                     protein 78 (E78) like is composed of two C4-type zinc
                     fingers; cd07165"
                     /db_xref="CDD:143539"
     Site            order(115,118,132,135,151,157,167,170)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143539"
     Site            order(125..127,133..134,136,138,141,144,164..165,168,171,
                     182,185)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143539"
     Site            order(162..163,165..166)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:143539"
     Region          330..529
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(336..337,340,343..344,373..374,377..378,381,415)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(345,348,352,357,362..363,365..366,369..370)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..538
                     /gene="Eip78C"
                     /coded_by="XM_034340337.2:2911..4527"
                     /db_xref="GeneID:117611898"
ORIGIN      
        1 mdvfkidqsl saglgsapap dtltpevsfd agsefnlsff dgpeenstqg fsdpgsvgsa
       61 saspppgtpg nipvnpsnaa lpivqvpsgi vikqeqhyaa adsnsstpak sfvacrvcgd
      121 kasgyhygvt scegckgffr rsiqkqieyr clrdgkclvi rlnrnrcqyc rfkkclavgm
      181 srdsvrygrv pkrsrergpe davptttttt attgvqqlts pgggnnnnnn nnnnnttsqn
      241 nnnssgqgqn aipcvpaaav veadqsdadv kqlavydvil qvsqahhahc gfteestrgi
      301 vrkpmiippa spedpeaass taetvesqri wlwqqfatrv tpsvqrvvef akrvpgfcdl
      361 sqddqlilik vgffevwlsh iskmttdssm tfedgtcitr qqmelmyepd fvsalmqfts
      421 slnghqlsdt elglfsgavl lgerpglndv kavqrlqdrl lealkvqalr nhtvsgeats
      481 aggcsgvssv sqripelral garhanlldw frrnwtklkl pplfaeifdi pkceedlq
//
LOCUS       XP_034196229             508 aa            linear   INV 03-SEP-2025
DEFINITION  ecdysone-induced protein 78C isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196229
VERSION     XP_034196229.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="ecdysone-induced protein 78C isoform X3"
                     /calculated_mol_wt=55527
     Region          79..159
                     /region_name="NR_DBD_DmE78_like"
                     /note="DNA-binding domain of Drosophila ecdysone-induced
                     protein 78 (E78) like is composed of two C4-type zinc
                     fingers; cd07165"
                     /db_xref="CDD:143539"
     Site            order(79,82,96,99,115,121,131,134)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143539"
     Site            order(89..91,97..98,100,102,105,108,128..129,132,135,146,
                     149)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143539"
     Site            order(126..127,129..130)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:143539"
     Region          300..499
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(306..307,310,313..314,343..344,347..348,351,385)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(315,318,322,327,332..333,335..336,339..340)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..508
                     /gene="Eip78C"
                     /coded_by="XM_034340338.2:855..2381"
                     /db_xref="GeneID:117611898"
ORIGIN      
        1 mmmdveetnt asnasssprp sspaadstfi svkrepstee psspleatsp gsilrnnneq
       61 hyaaadsnss tpaksfvacr vcgdkasgyh ygvtscegck gffrrsiqkq ieyrclrdgk
      121 clvirlnrnr cqycrfkkcl avgmsrdsvr ygrvpkrsre rgpedavptt ttttattgvq
      181 qltspgggnn nnnnnnnnnt tsqnnnnssg qgqnaipcvp aaavveadqs dadvkqlavy
      241 dvilqvsqah hahcgftees trgivrkpmi ipastssqpa spedpeaass taetvesqri
      301 wlwqqfatrv tpsvqrvvef akrvpgfcdl sqddqlilik vgffevwlsh iskmttdssm
      361 tfedgtcitr qqmelmyepd fvsalmqfts slnghqlsdt elglfsgavl lgerpglndv
      421 kavqrlqdrl lealkvqalr nhtvsgeats aggcsgvssv sqripelral garhanlldw
      481 frrnwtklkl pplfaeifdi pkceedlq
//
LOCUS       XP_076546778             365 aa            linear   INV 03-SEP-2025
DEFINITION  putative protein phosphatase 2C T23F11.1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546778
VERSION     XP_076546778.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690663.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..365
                     /product="putative protein phosphatase 2C T23F11.1 isoform
                     X1"
                     /calculated_mol_wt=41216
     Region          71..344
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(82,86..87,108..110,277,335)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..365
                     /gene="LOC117611901"
                     /coded_by="XM_076690663.1:87..1184"
                     /db_xref="GeneID:117611901"
ORIGIN      
        1 mvhqevilgy ihltayegkn iifedyfynv eswnntmrin fckriikkim ghtlsepvtt
       61 rksvycrnsn yrvgsscmqg wrikmedchv hilslpddpg taffavydgh ggaamalhag
      121 khlheyiirr seykagnivq ameqgfleld kamqhdvtlk deqagttvia llvkdnilys
      181 anagdsrsva sikghavpls rdhkptlkde rerieaaggw vefnrvngll slsralgdft
      241 fkrnerkspq eqivtafpev qqfqitedwe fvvlacdgiw dvmtsnevvn firarlmqpk
      301 fgtgqendtm dpeeiceeli qhclapdplm gtgcdnmtvv lvcflhgkpy sylisrcres
      361 sstnp
//
LOCUS       XP_034196239             316 aa            linear   INV 03-SEP-2025
DEFINITION  putative protein phosphatase 2C T23F11.1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196239
VERSION     XP_034196239.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340348.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196239.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="putative protein phosphatase 2C T23F11.1 isoform
                     X2"
                     /calculated_mol_wt=35237
     Region          22..295
                     /region_name="PP2Cc"
                     /note="Serine/threonine phosphatases, family 2C, catalytic
                     domain; The protein architecture and deduced catalytic
                     mechanism of PP2C phosphatases are similar to the PP1,
                     PP2A, PP2B family of protein Ser/Thr phosphatases, with
                     which PP2C shares no sequence...; cd00143"
                     /db_xref="CDD:238083"
     Site            order(33,37..38,59..61,228,286)
                     /site_type="active"
                     /db_xref="CDD:238083"
     CDS             1..316
                     /gene="LOC117611901"
                     /coded_by="XM_034340348.2:158..1108"
                     /db_xref="GeneID:117611901"
ORIGIN      
        1 mghtlsepvt trksvycrns nyrvgsscmq gwrikmedch vhilslpddp gtaffavydg
       61 hggaamalha gkhlheyiir rseykagniv qameqgflel dkamqhdvtl kdeqagttvi
      121 allvkdnily sanagdsrsv asikghavpl srdhkptlkd ererieaagg wvefnrvngl
      181 lslsralgdf tfkrnerksp qeqivtafpe vqqfqitedw efvvlacdgi wdvmtsnevv
      241 nfirarlmqp kfgtgqendt mdpeeiceel iqhclapdpl mgtgcdnmtv vlvcflhgkp
      301 ysylisrcre ssstnp
//
LOCUS       XP_034196230             346 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611899 [Osmia lignaria lignaria].
ACCESSION   XP_034196230
VERSION     XP_034196230.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="uncharacterized protein LOC117611899"
                     /calculated_mol_wt=40714
     Region          33..>83
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          33..52
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(37,39,41,43..44,47..48,51,67,69,73..74,77..78,81,96,
                     98,100,102..103,106..107,110)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <54..163
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          60..82
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          91..110
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          119..139
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          146..166
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          201..221
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(206,208,210,212..213,216..217,220,237,239,243..244,
                     247..248,251,265,267,269,271..272,275..276,280)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          230..252
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          260..281
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..346
                     /gene="LOC117611899"
                     /coded_by="XM_034340339.2:142..1182"
                     /db_xref="GeneID:117611899"
ORIGIN      
        1 mgqdrnsetd lientnticf qakkqkrhec typgckaify rpsrlarhvr lhtgekshkc
       61 nypncdkayt nsshlkrhme thnttktifq ctecslfivh rhnlkrhyrt qhdrgkltck
      121 icnesftkky qlathmathg svsyicekcg ksfisnpklk rhreihekry pctvpgcnev
      181 lekwllfrah lktqhvkshk ctycgkvfls kshlkmhfqv hmkhrlvlsc pydkcprvyc
      241 fqknldqhir vhhleqkyec dicklkissk aklrnhiqnl hvsvkriqkt rksqrkrkdi
      301 glpkrsmvsk ltgvilppqv ekiilerevv rssetyetvl ntdsdl
//
LOCUS       XP_034196231             346 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611899 [Osmia lignaria lignaria].
ACCESSION   XP_034196231
VERSION     XP_034196231.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340340.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="uncharacterized protein LOC117611899"
                     /calculated_mol_wt=40714
     Region          33..>83
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          33..52
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(37,39,41,43..44,47..48,51,67,69,73..74,77..78,81,96,
                     98,100,102..103,106..107,110)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <54..163
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          60..82
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          91..110
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          119..139
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          146..166
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          201..221
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(206,208,210,212..213,216..217,220,237,239,243..244,
                     247..248,251,265,267,269,271..272,275..276,280)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          230..252
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          260..281
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..346
                     /gene="LOC117611899"
                     /coded_by="XM_034340340.2:129..1169"
                     /db_xref="GeneID:117611899"
ORIGIN      
        1 mgqdrnsetd lientnticf qakkqkrhec typgckaify rpsrlarhvr lhtgekshkc
       61 nypncdkayt nsshlkrhme thnttktifq ctecslfivh rhnlkrhyrt qhdrgkltck
      121 icnesftkky qlathmathg svsyicekcg ksfisnpklk rhreihekry pctvpgcnev
      181 lekwllfrah lktqhvkshk ctycgkvfls kshlkmhfqv hmkhrlvlsc pydkcprvyc
      241 fqknldqhir vhhleqkyec dicklkissk aklrnhiqnl hvsvkriqkt rksqrkrkdi
      301 glpkrsmvsk ltgvilppqv ekiilerevv rssetyetvl ntdsdl
//
LOCUS       XP_034196233             337 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611900 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196233
VERSION     XP_034196233.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340342.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="uncharacterized protein LOC117611900 isoform X1"
                     /calculated_mol_wt=38590
     Region          137..218
                     /region_name="HFD_SUPT7L"
                     /note="histone-fold domain found in Suppressor of Ty
                     7-like (SUPT7L) and similar proteins; cd06847"
                     /db_xref="CDD:467022"
     CDS             1..337
                     /gene="LOC117611900"
                     /coded_by="XM_034340342.2:301..1314"
                     /db_xref="GeneID:117611900"
ORIGIN      
        1 msmkvltmks nknsgtgslw gelparetlk qevitpdiie nvwrqvmees nntdcdfqmd
       61 rqpeyiqlpm gnlhvlntin lhqqlrmmee gvvlgnmtti etktsqegke tqnsfhflpm
      121 qkkrnmfkds stihhltpqt arcilrhavi illahigfek ssdiaietlt diadhflkrm
      181 tllikvayeq kdhgfpdiie rvlletgvgn vstlhdyyqe yvlkfeenmk kkveammekq
      241 rqlelnacss kmvldeaank lqfeeldefg nvyrevptlq lldpemgfpp sldagfqmly
      301 sleqdelnnl eveeeevnvs dspsvgqrsd tsdkkks
//
LOCUS       XP_034196234             330 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611900 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196234
VERSION     XP_034196234.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340343.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="uncharacterized protein LOC117611900 isoform X2"
                     /calculated_mol_wt=37799
     Region          130..211
                     /region_name="HFD_SUPT7L"
                     /note="histone-fold domain found in Suppressor of Ty
                     7-like (SUPT7L) and similar proteins; cd06847"
                     /db_xref="CDD:467022"
     CDS             1..330
                     /gene="LOC117611900"
                     /coded_by="XM_034340343.2:150..1142"
                     /db_xref="GeneID:117611900"
ORIGIN      
        1 mksnknsgtg slwgelpare tlkqevitpd iienvwrqvm eesnntdcdf qmdrqpeyiq
       61 lpmgnlhvln tinlhqqlrm meegvvlgnm ttietktsqe gketqnsfhf lpmqkkrnmf
      121 kdsstihhlt pqtarcilrh aviillahig fekssdiaie tltdiadhfl krmtllikva
      181 yeqkdhgfpd iiervlletg vgnvstlhdy yqeyvlkfee nmkkkveamm ekqrqlelna
      241 csskmvldea anklqfeeld efgnvyrevp tlqlldpemg fppsldagfq mlysleqdel
      301 nnleveeeev nvsdspsvgq rsdtsdkkks
//
LOCUS       XP_034196235             330 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611900 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196235
VERSION     XP_034196235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340344.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="uncharacterized protein LOC117611900 isoform X2"
                     /calculated_mol_wt=37799
     Region          130..211
                     /region_name="HFD_SUPT7L"
                     /note="histone-fold domain found in Suppressor of Ty
                     7-like (SUPT7L) and similar proteins; cd06847"
                     /db_xref="CDD:467022"
     CDS             1..330
                     /gene="LOC117611900"
                     /coded_by="XM_034340344.2:196..1188"
                     /db_xref="GeneID:117611900"
ORIGIN      
        1 mksnknsgtg slwgelpare tlkqevitpd iienvwrqvm eesnntdcdf qmdrqpeyiq
       61 lpmgnlhvln tinlhqqlrm meegvvlgnm ttietktsqe gketqnsfhf lpmqkkrnmf
      121 kdsstihhlt pqtarcilrh aviillahig fekssdiaie tltdiadhfl krmtllikva
      181 yeqkdhgfpd iiervlletg vgnvstlhdy yqeyvlkfee nmkkkveamm ekqrqlelna
      241 csskmvldea anklqfeeld efgnvyrevp tlqlldpemg fppsldagfq mlysleqdel
      301 nnleveeeev nvsdspsvgq rsdtsdkkks
//
LOCUS       XP_034196236             330 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611900 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196236
VERSION     XP_034196236.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340345.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="uncharacterized protein LOC117611900 isoform X3"
                     /calculated_mol_wt=37889
     Region          137..218
                     /region_name="HFD_SUPT7L"
                     /note="histone-fold domain found in Suppressor of Ty
                     7-like (SUPT7L) and similar proteins; cd06847"
                     /db_xref="CDD:467022"
     CDS             1..330
                     /gene="LOC117611900"
                     /coded_by="XM_034340345.2:301..1293"
                     /db_xref="GeneID:117611900"
ORIGIN      
        1 msmkvltmks nknsgtgslw gelparetlk qevitpdiie nvwrqvmees nntdcdfqmd
       61 rqpeyiqlpm gnlhvlntin lhqqlrmmee gvvlgnmtti etktsqegke tqnsfhflpm
      121 qkkrnmfkds stihhltpqt arcilrhavi illahigfek ssdiaietlt diadhflkrm
      181 tllikvayeq kdhgfpdiie rvlletgvgn vstlhdyyqe yvlkfeenmk kkveammekq
      241 rqlelnayea anklqfeeld efgnvyrevp tlqlldpemg fppsldagfq mlysleqdel
      301 nnleveeeev nvsdspsvgq rsdtsdkkks
//
LOCUS       XP_034196237             327 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611900 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196237
VERSION     XP_034196237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340346.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..327
                     /product="uncharacterized protein LOC117611900 isoform X4"
                     /calculated_mol_wt=37516
     Region          137..218
                     /region_name="HFD_SUPT7L"
                     /note="histone-fold domain found in Suppressor of Ty
                     7-like (SUPT7L) and similar proteins; cd06847"
                     /db_xref="CDD:467022"
     CDS             1..327
                     /gene="LOC117611900"
                     /coded_by="XM_034340346.2:301..1284"
                     /db_xref="GeneID:117611900"
ORIGIN      
        1 msmkvltmks nknsgtgslw gelparetlk qevitpdiie nvwrqvmees nntdcdfqmd
       61 rqpeyiqlpm gnlhvlntin lhqqlrmmee gvvlgnmtti etktsqegke tqnsfhflpm
      121 qkkrnmfkds stihhltpqt arcilrhavi illahigfek ssdiaietlt diadhflkrm
      181 tllikvayeq kdhgfpdiie rvlletgvgn vstlhdyyqe yvlkfeenmk kkveammekq
      241 rmvldeaank lqfeeldefg nvyrevptlq lldpemgfpp sldagfqmly sleqdelnnl
      301 eveeeevnvs dspsvgqrsd tsdkkks
//
LOCUS       XP_034196240             240 aa            linear   INV 03-SEP-2025
DEFINITION  MIT domain-containing protein 1-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196240
VERSION     XP_034196240.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340349.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="MIT domain-containing protein 1-like isoform X1"
                     /calculated_mol_wt=27238
     Region          1..76
                     /region_name="MIT"
                     /note="domain contained within Microtubule Interacting and
                     Trafficking molecules. The MIT domain is found in sorting
                     nexins, the nuclear thiol protease PalBH, the AAA protein
                     spastin and archaebacterial proteins with similar domain
                     architecture, vacuolar...; cl00299"
                     /db_xref="CDD:469712"
     Region          90..226
                     /region_name="MIT_C"
                     /note="Phospholipase D-like domain at C-terminus of MIT;
                     pfam16565"
                     /db_xref="CDD:465180"
     CDS             1..240
                     /gene="LOC117611902"
                     /coded_by="XM_034340349.2:124..846"
                     /db_xref="GeneID:117611902"
ORIGIN      
        1 mesaaasilk ravemdnnkq ytmalvlyqe gvqilldsmk etkgsamqkh fatkaseyld
       61 raekikklie rekaagtyre likiesgntg ygyatvfgrf ldatvtyihi edpyirafhq
      121 cqnlvrlcel avrkchalsk islvttldtd dkknqiarle elkksltshl isfefkfsdt
      181 lhdrqillsn gwiikigrgl dyfkapdgkf vlgacdlelr pcletiidif htehlktdit
//
LOCUS       XP_034196241             194 aa            linear   INV 03-SEP-2025
DEFINITION  MIT domain-containing protein 1-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196241
VERSION     XP_034196241.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340350.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..194
                     /product="MIT domain-containing protein 1-like isoform X2"
                     /calculated_mol_wt=22178
     Region          <2..30
                     /region_name="MIT"
                     /note="domain contained within Microtubule Interacting and
                     Trafficking molecules. The MIT domain is found in sorting
                     nexins, the nuclear thiol protease PalBH, the AAA protein
                     spastin and archaebacterial proteins with similar domain
                     architecture, vacuolar...; cl00299"
                     /db_xref="CDD:469712"
     Region          44..180
                     /region_name="MIT_C"
                     /note="Phospholipase D-like domain at C-terminus of MIT;
                     pfam16565"
                     /db_xref="CDD:465180"
     CDS             1..194
                     /gene="LOC117611902"
                     /coded_by="XM_034340350.2:169..753"
                     /db_xref="GeneID:117611902"
ORIGIN      
        1 mqkhfatkas eyldraekik klierekaag tyrelikies gntgygyatv fgrfldatvt
       61 yihiedpyir afhqcqnlvr lcelavrkch alskislvtt ldtddkknqi arleelkksl
      121 tshlisfefk fsdtlhdrqi llsngwiiki grgldyfkap dgkfvlgacd lelrpcleti
      181 idifhtehlk tdit
//
LOCUS       XP_034196073            1941 aa            linear   INV 03-SEP-2025
DEFINITION  Down syndrome cell adhesion molecule 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196073
VERSION     XP_034196073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1941
                     /product="Down syndrome cell adhesion molecule 2 isoform
                     X1"
                     /calculated_mol_wt=210713
     Region          29..127
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          48..52
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          61..65
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          85..89
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          105..110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          237..314
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          330..417
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          348..352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          421..516
                     /region_name="IgI_4_Dscam"
                     /note="Fourth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20956"
                     /db_xref="CDD:409548"
     Region          421..424
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409548"
     Region          430..434
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409548"
     Region          437..446
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409548"
     Region          451..457
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409548"
     Region          459..462
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409548"
     Region          467..475
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409548"
     Region          479..488
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409548"
     Region          495..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409548"
     Region          506..516
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409548"
     Region          520..607
                     /region_name="IgI_5_Dscam"
                     /note="Fifth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20958"
                     /db_xref="CDD:409550"
     Region          520..522
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409550"
     Region          527..531
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409550"
     Region          534..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409550"
     Region          548..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409550"
     Region          555..557
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409550"
     Region          564..568
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409550"
     Region          571..577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409550"
     Region          585..593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409550"
     Region          597..607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409550"
     Region          610..707
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          628..632
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          673..677
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          687..692
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          700..703
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          710..805
                     /region_name="IgI_7_Dscam"
                     /note="Seventh immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20954"
                     /db_xref="CDD:409546"
     Region          710..714
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409546"
     Region          719..723
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409546"
     Region          726..735
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409546"
     Region          741..747
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409546"
     Region          753..756
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409546"
     Region          764..767
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409546"
     Region          770..776
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409546"
     Region          783..791
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409546"
     Region          796..805
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409546"
     Region          815..905
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          826..830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          839..843
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          871..875
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          885..890
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          898..901
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          909..1002
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(909,975,990)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <971..1315
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(991..992,994..995)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1332..1392
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          1332..1336
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1368..1372
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1382..1387
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            order(1406,1468,1483)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          1421..1483
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          1500..1580
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1500,1565,1580)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..1941
                     /gene="Dscam2"
                     /coded_by="XM_034340182.2:452..6277"
                     /db_xref="GeneID:117611830"
ORIGIN      
        1 mlarclicig avaavtsagg hgfdahlrgp sfviepssrv efsnssgawl dctatgsppp
       61 nidwstadgh pvndvsgvrr vlrngtlvll pfpaaafrqd vhsaayrcva snsvgrvlsr
      121 dvqvravvaq aykvdvevig gasrgctavl rcvvpsfvkd lvrvvswlqe psfyiypslq
      181 gdgkfhllpt gellvhslef ndqihgyrcr tmhrltrqvv vssmanvria dhrgvmppvi
      241 lensgvvhva qdestslvcv aqacptpeyr wyaqtgsepm lvlsgprtrl lgsvlaleav
      301 tledsgiyrc saanpggeas aeirlivtap lhvevtppll svhlggnaef rcevsthpqa
      361 gphfvtwykd grqlpgtgrq sellrlngig redrgmyqci vrraegdtaq asaelqlgda
      421 ppmllysfie qtlqpgpavs lkcsaagnpt pqvswaldgf plptngrfvi gqyvtvhgdv
      481 ishvnishvm vedggeysct aenragkvth aarlnvyglp yirlipkvta vagetlrlkc
      541 pvagypieei kweranrelp ddlrqkvlpd gtlvitsvqk kgdagvytcs arnkqghsar
      601 rsgdvavivp piiepftfqe glsegmrtrt vcgvaagdpp ltiswlkdgq tpfplppnla
      661 svanvsqldp yssllsitnl aaehsgdytc vaanpaaevr ytaklqvkvp prwiveptdv
      721 svernkhval hcqaqgvptp tivwkkatgs ksgeyeelre raytkilsng tlllqhvked
      781 regfylcqas ngigsgigkv vqlkvnsspy faapsrlvtv kkgdtatlhc evhgdtpvtv
      841 twlkggkiel npstnyrvtv krevtpdgvi aqlqissaea sdsgayfcqa snlygrdqql
      901 vqllvqeppq ppnsletamv asrsinvkwq hksqdssevt kyilqykegd ggmwqqqeft
      961 gpplpyaali delkpatryt irviaegpag rsvpsaelvv rtepqrpagp pinlaarals
     1021 sseilitwsp plpelrhgdi qgfnvgyret ssanpsynfs svsgdgeegg aelrltglrp
     1081 ytkytlvvqa ynqvgsgpls eplltqtled vpsmppedvr caaltsqslq vtwepppnth
     1141 sngiiqgykl hyepiladiw rsvdemevrk tsalttvltg lrkytnytiq vlaftrvgdg
     1201 vptiatycqt eedvpgspad ikvvvsspqa lfiswlpple pngiitkynl ytrvvdgree
     1261 lnhgkktlpa tntffeatdl qqhveyqfwv tgstrvgegq ssrvaaqvpt nrvparitsf
     1321 gghvvrpwrg satlacnavg dptrewykgv aeqirtdttr niqilpsgel vlsnlqsqdg
     1381 gnytcqvena qgsdklhytl tvqvppsapv lyvtsstsss illhwkpghs ggapltgytl
     1441 hyrtthgnld elqlsrhats helkgllcgn tyqlyltshn kigsspsspv lsvrtqgqap
     1501 gippaaafls pnsttlvlrl hvwpdngcpi lyfviqyrpi nefhwtlvsn svkmqrrfvv
     1561 tnlqpssvyq lkveahnvag snqaeftfvt ltkegepppp elskrgitsa vpfyadvkvm
     1621 lpliaatial lvavvivalr wrsrylgerm qrpmketqen qqnaetqrer yyatihkval
     1681 qqgagpdkip gepllrygrq petaedispy atfqlseggg gslaglgglg glgsaaeasa
     1741 aglhpnntll hsfmyheham tegcaspppa atsmksvssr rrqqrkqqtp gdvesdeses
     1801 dpdqltssrt essnqldagk lkhiravsdf iyhgtsstss dispmseqks lprrgrsrwh
     1861 vpsrsslrtl lppisvaett fvganqgnvv pgngdradrp elseaecdid slkklklglr
     1921 sslwsrpstq nnpssdysia v
//
LOCUS       XP_034196074            1936 aa            linear   INV 03-SEP-2025
DEFINITION  Down syndrome cell adhesion molecule 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196074
VERSION     XP_034196074.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340183.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1936
                     /product="Down syndrome cell adhesion molecule 2 isoform
                     X2"
                     /calculated_mol_wt=210634
     Region          29..127
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          48..52
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          61..65
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          85..89
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          105..110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          237..314
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          330..417
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          348..352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          421..516
                     /region_name="IgI_4_Dscam"
                     /note="Fourth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20956"
                     /db_xref="CDD:409548"
     Region          421..424
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409548"
     Region          430..434
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409548"
     Region          437..446
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409548"
     Region          451..457
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409548"
     Region          459..462
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409548"
     Region          467..475
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409548"
     Region          479..488
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409548"
     Region          495..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409548"
     Region          506..516
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409548"
     Region          520..607
                     /region_name="IgI_5_Dscam"
                     /note="Fifth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20958"
                     /db_xref="CDD:409550"
     Region          520..522
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409550"
     Region          527..531
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409550"
     Region          534..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409550"
     Region          548..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409550"
     Region          555..557
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409550"
     Region          564..568
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409550"
     Region          571..577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409550"
     Region          585..593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409550"
     Region          597..607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409550"
     Region          610..702
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          628..632
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          668..672
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          682..687
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          695..698
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          705..800
                     /region_name="IgI_7_Dscam"
                     /note="Seventh immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20954"
                     /db_xref="CDD:409546"
     Region          705..709
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409546"
     Region          714..718
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409546"
     Region          721..730
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409546"
     Region          736..742
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409546"
     Region          748..751
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409546"
     Region          759..762
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409546"
     Region          765..771
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409546"
     Region          778..786
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409546"
     Region          791..800
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409546"
     Region          810..900
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          821..825
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          834..838
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          866..870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          880..885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          893..896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          904..997
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(904,970,985)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <966..1310
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(986..987,989..990)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1327..1387
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          1327..1331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1363..1367
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1377..1382
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            order(1401,1463,1478)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          1416..1478
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          1495..1575
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1495,1560,1575)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..1936
                     /gene="Dscam2"
                     /coded_by="XM_034340183.2:452..6262"
                     /db_xref="GeneID:117611830"
ORIGIN      
        1 mlarclicig avaavtsagg hgfdahlrgp sfviepssrv efsnssgawl dctatgsppp
       61 nidwstadgh pvndvsgvrr vlrngtlvll pfpaaafrqd vhsaayrcva snsvgrvlsr
      121 dvqvravvaq aykvdvevig gasrgctavl rcvvpsfvkd lvrvvswlqe psfyiypslq
      181 gdgkfhllpt gellvhslef ndqihgyrcr tmhrltrqvv vssmanvria dhrgvmppvi
      241 lensgvvhva qdestslvcv aqacptpeyr wyaqtgsepm lvlsgprtrl lgsvlaleav
      301 tledsgiyrc saanpggeas aeirlivtap lhvevtppll svhlggnaef rcevsthpqa
      361 gphfvtwykd grqlpgtgrq sellrlngig redrgmyqci vrraegdtaq asaelqlgda
      421 ppmllysfie qtlqpgpavs lkcsaagnpt pqvswaldgf plptngrfvi gqyvtvhgdv
      481 ishvnishvm vedggeysct aenragkvth aarlnvyglp yirlipkvta vagetlrlkc
      541 pvagypieei kweranrelp ddlrqkvlpd gtlvitsvqk kgdagvytcs arnkqghsar
      601 rsgdvavivp pkispfttdr dihlgerttl tcsvtrgdlp ltiswlkdgr amgpservtv
      661 tnmdqynsil mieslspdhn gnyscvarnl aaevshtqrl vvhvpprwiv eptdvsvern
      721 khvalhcqaq gvptptivwk katgsksgey eelreraytk ilsngtlllq hvkedregfy
      781 lcqasngigs gigkvvqlkv nsspyfaaps rlvtvkkgdt atlhcevhgd tpvtvtwlkg
      841 gkielnpstn yrvtvkrevt pdgviaqlqi ssaeasdsga yfcqasnlyg rdqqlvqllv
      901 qeppqppnsl etamvasrsi nvkwqhksqd ssevtkyilq ykegdggmwq qqeftgpplp
      961 yaalidelkp atrytirvia egpagrsvps aelvvrtepq rpagppinla aralssseil
     1021 itwspplpel rhgdiqgfnv gyretssanp synfssvsgd geeggaelrl tglrpytkyt
     1081 lvvqaynqvg sgplsepllt qtledvpsmp pedvrcaalt sqslqvtwep ppnthsngii
     1141 qgyklhyepi ladiwrsvde mevrktsalt tvltglrkyt nytiqvlaft rvgdgvptia
     1201 tycqteedvp gspadikvvv sspqalfisw lpplepngii tkynlytrvv dgreelnhgk
     1261 ktlpatntff eatdlqqhve yqfwvtgstr vgegqssrva aqvptnrvpa ritsfgghvv
     1321 rpwrgsatla cnavgdptre wykgvaeqir tdttrniqil psgelvlsnl qsqdggnytc
     1381 qvenaqgsdk lhytltvqvp psapvlyvts stsssillhw kpghsggapl tgytlhyrtt
     1441 hgnldelqls rhatshelkg llcgntyqly ltshnkigss psspvlsvrt qgqapgippa
     1501 aaflspnstt lvlrlhvwpd ngcpilyfvi qyrpinefhw tlvsnsvkmq rrfvvtnlqp
     1561 ssvyqlkvea hnvagsnqae ftfvtltkeg eppppelskr gitsavpfya dvkvmlplia
     1621 atiallvavv ivalrwrsry lgermqrpmk etqenqqnae tqreryyati hkvalqqgag
     1681 pdkipgepll rygrqpetae dispyatfql seggggslag lgglgglgsa aeasaaglhp
     1741 nntllhsfmy hehamtegca spppaatsmk svssrrrqqr kqqtpgdves desesdpdql
     1801 tssrtessnq ldagklkhir avsdfiyhgt sstssdispm seqkslprrg rsrwhvpsrs
     1861 slrtllppis vaettfvgan qgnvvpgngd radrpelsea ecdidslkkl klglrsslws
     1921 rpstqnnpss dysiav
//
LOCUS       XP_076546779            1935 aa            linear   INV 03-SEP-2025
DEFINITION  Down syndrome cell adhesion molecule 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546779
VERSION     XP_076546779.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690664.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1935
                     /product="Down syndrome cell adhesion molecule 2 isoform
                     X3"
                     /calculated_mol_wt=209950
     Region          29..127
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          48..52
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409547"
     Region          61..65
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409547"
     Region          85..89
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409547"
     Region          105..110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409547"
     Region          118..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409547"
     Region          237..314
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          330..417
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          348..352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          421..516
                     /region_name="IgI_4_Dscam"
                     /note="Fourth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20956"
                     /db_xref="CDD:409548"
     Region          421..424
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409548"
     Region          430..434
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409548"
     Region          437..446
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409548"
     Region          451..457
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409548"
     Region          459..462
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409548"
     Region          467..475
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409548"
     Region          479..488
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409548"
     Region          495..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409548"
     Region          506..516
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409548"
     Region          520..607
                     /region_name="IgI_5_Dscam"
                     /note="Fifth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20958"
                     /db_xref="CDD:409550"
     Region          520..522
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409550"
     Region          527..531
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409550"
     Region          534..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409550"
     Region          548..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409550"
     Region          555..557
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409550"
     Region          564..568
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409550"
     Region          571..577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409550"
     Region          585..593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409550"
     Region          597..607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409550"
     Region          610..707
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          628..632
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          673..677
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          687..692
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          700..703
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          710..805
                     /region_name="IgI_7_Dscam"
                     /note="Seventh immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20954"
                     /db_xref="CDD:409546"
     Region          710..714
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409546"
     Region          719..723
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409546"
     Region          726..735
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409546"
     Region          741..747
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409546"
     Region          753..756
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409546"
     Region          764..767
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409546"
     Region          770..776
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409546"
     Region          783..791
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409546"
     Region          796..805
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409546"
     Region          815..905
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          826..830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          839..843
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          871..875
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          885..890
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          898..901
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          909..1002
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(909,975,990)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <971..1315
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(991..992,994..995)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1332..1392
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          1332..1336
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1368..1372
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1382..1387
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            order(1406,1468,1483)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          1421..1483
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          1500..1580
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1500,1565,1580)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..1935
                     /gene="Dscam2"
                     /coded_by="XM_076690664.1:452..6259"
                     /db_xref="GeneID:117611830"
ORIGIN      
        1 mlarclicig avaavtsagg hgfdahlrgp sfviepssrv efsnssgawl dctatgsppp
       61 nidwstadgh pvndvsgvrr vlrngtlvll pfpaaafrqd vhsaayrcva snsvgrvlsr
      121 dvqvravvaq aykvdvevig gasrgctavl rcvvpsfvkd lvrvvswlqe psfyiypslq
      181 gdgkfhllpt gellvhslef ndqihgyrcr tmhrltrqvv vssmanvria dhrgvmppvi
      241 lensgvvhva qdestslvcv aqacptpeyr wyaqtgsepm lvlsgprtrl lgsvlaleav
      301 tledsgiyrc saanpggeas aeirlivtap lhvevtppll svhlggnaef rcevsthpqa
      361 gphfvtwykd grqlpgtgrq sellrlngig redrgmyqci vrraegdtaq asaelqlgda
      421 ppmllysfie qtlqpgpavs lkcsaagnpt pqvswaldgf plptngrfvi gqyvtvhgdv
      481 ishvnishvm vedggeysct aenragkvth aarlnvyglp yirlipkvta vagetlrlkc
      541 pvagypieei kweranrelp ddlrqkvlpd gtlvitsvqk kgdagvytcs arnkqghsar
      601 rsgdvavivp piiepftfqe glsegmrtrt vcgvaagdpp ltiswlkdgq tpfplppnla
      661 svanvsqldp yssllsitnl aaehsgdytc vaanpaaevr ytaklqvkvp prwiveptdv
      721 svernkhval hcqaqgvptp tivwkkatgs ksgeyeelre raytkilsng tlllqhvked
      781 regfylcqas ngigsgigkv vqlkvnsspy faapsrlvtv kkgdtatlhc evhgdtpvtv
      841 twlkggkiel npstnyrvtv krevtpdgvi aqlqissaea sdsgayfcqa snlygrdqql
      901 vqllvqeppq ppnsletamv asrsinvkwq hksqdssevt kyilqykegd ggmwqqqeft
      961 gpplpyaali delkpatryt irviaegpag rsvpsaelvv rtepqrpagp pinlaarals
     1021 sseilitwsp plpelrhgdi qgfnvgyret ssanpsynfs svsgdgeegg aelrltglrp
     1081 ytkytlvvqa ynqvgsgpls eplltqtled vpsmppedvr caaltsqslq vtwepppnth
     1141 sngiiqgykl hyepiladiw rsvdemevrk tsalttvltg lrkytnytiq vlaftrvgdg
     1201 vptiatycqt eedvpgspad ikvvvsspqa lfiswlpple pngiitkynl ytrvvdgree
     1261 lnhgkktlpa tntffeatdl qqhveyqfwv tgstrvgegq ssrvaaqvpt nrvparitsf
     1321 gghvvrpwrg satlacnavg dptrewykgv aeqirtdttr niqilpsgel vlsnlqsqdg
     1381 gnytcqvena qgsdklhytl tvqvppsapv lyvtsstsss illhwkpghs ggapltgytl
     1441 hyrtthgnld elqlsrhats helkgllcgn tyqlyltshn kigsspsspv lsvrtqgqap
     1501 gippaaafls pnsttlvlrl hvwpdngcpi lyfviqyrpi nefhwtlvsn svkmqrrfvv
     1561 tnlqpssvyq lkveahnvag snqaeftfvt ltkegepppp elskrgitsa vpfyadvkvm
     1621 lpliaatial lvavvivalr wrsrylgerm qrpmketqen qqnaetqrer yyatihkval
     1681 qqgagpdkip gepllrygrq petaedispy atfqlseggg gslaglgglg glgsaaeasa
     1741 aglhpnntll hsfmyheham tegcaspppa atsmksvssr rrqqrkqqtp gdvesdeses
     1801 dpdqltssrt essnqldagk lkhiravsdf iyhgtsstss dispmseqks lprrgrsrss
     1861 lrtllppisv aettfvganq gnvvpgngdr adrpelseae cdidslkklk lglrsslwsr
     1921 pstqnnpssd ysiav
//
LOCUS       XP_076546780            1930 aa            linear   INV 03-SEP-2025
DEFINITION  Down syndrome cell adhesion molecule 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546780
VERSION     XP_076546780.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690665.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1930
                     /product="Down syndrome cell adhesion molecule 2 isoform
                     X4"
                     /calculated_mol_wt=209446
     Region          29..127
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          48..52
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409547"
     Region          61..65
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409547"
     Region          85..89
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409547"
     Region          105..110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409547"
     Region          118..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409547"
     Region          237..314
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          330..417
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          348..352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          421..516
                     /region_name="IgI_4_Dscam"
                     /note="Fourth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20956"
                     /db_xref="CDD:409548"
     Region          421..424
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409548"
     Region          430..434
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409548"
     Region          437..446
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409548"
     Region          451..457
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409548"
     Region          459..462
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409548"
     Region          467..475
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409548"
     Region          479..488
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409548"
     Region          495..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409548"
     Region          506..516
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409548"
     Region          520..607
                     /region_name="IgI_5_Dscam"
                     /note="Fifth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20958"
                     /db_xref="CDD:409550"
     Region          520..522
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409550"
     Region          527..531
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409550"
     Region          534..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409550"
     Region          548..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409550"
     Region          555..557
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409550"
     Region          564..568
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409550"
     Region          571..577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409550"
     Region          585..593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409550"
     Region          597..607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409550"
     Region          610..707
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          628..632
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          673..677
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          687..692
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          700..703
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          710..805
                     /region_name="IgI_7_Dscam"
                     /note="Seventh immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20954"
                     /db_xref="CDD:409546"
     Region          710..714
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409546"
     Region          719..723
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409546"
     Region          726..735
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409546"
     Region          741..747
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409546"
     Region          753..756
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409546"
     Region          764..767
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409546"
     Region          770..776
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409546"
     Region          783..791
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409546"
     Region          796..805
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409546"
     Region          815..905
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          826..830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          839..843
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          871..875
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          885..890
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          898..901
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          909..1002
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(909,975,990)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <971..1315
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(991..992,994..995)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1332..1392
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          1332..1336
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1368..1372
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1382..1387
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            order(1406,1468,1483)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          1421..1483
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          1500..1580
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1500,1565,1580)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..1930
                     /gene="Dscam2"
                     /coded_by="XM_076690665.1:452..6244"
                     /db_xref="GeneID:117611830"
ORIGIN      
        1 mlarclicig avaavtsagg hgfdahlrgp sfviepssrv efsnssgawl dctatgsppp
       61 nidwstadgh pvndvsgvrr vlrngtlvll pfpaaafrqd vhsaayrcva snsvgrvlsr
      121 dvqvravvaq aykvdvevig gasrgctavl rcvvpsfvkd lvrvvswlqe psfyiypslq
      181 gdgkfhllpt gellvhslef ndqihgyrcr tmhrltrqvv vssmanvria dhrgvmppvi
      241 lensgvvhva qdestslvcv aqacptpeyr wyaqtgsepm lvlsgprtrl lgsvlaleav
      301 tledsgiyrc saanpggeas aeirlivtap lhvevtppll svhlggnaef rcevsthpqa
      361 gphfvtwykd grqlpgtgrq sellrlngig redrgmyqci vrraegdtaq asaelqlgda
      421 ppmllysfie qtlqpgpavs lkcsaagnpt pqvswaldgf plptngrfvi gqyvtvhgdv
      481 ishvnishvm vedggeysct aenragkvth aarlnvyglp yirlipkvta vagetlrlkc
      541 pvagypieei kweranrelp ddlrqkvlpd gtlvitsvqk kgdagvytcs arnkqghsar
      601 rsgdvavivp piiepftfqe glsegmrtrt vcgvaagdpp ltiswlkdgq tpfplppnla
      661 svanvsqldp yssllsitnl aaehsgdytc vaanpaaevr ytaklqvkvp prwiveptdv
      721 svernkhval hcqaqgvptp tivwkkatgs ksgeyeelre raytkilsng tlllqhvked
      781 regfylcqas ngigsgigkv vqlkvnsspy faapsrlvtv kkgdtatlhc evhgdtpvtv
      841 twlkggkiel npstnyrvtv krevtpdgvi aqlqissaea sdsgayfcqa snlygrdqql
      901 vqllvqeppq ppnsletamv asrsinvkwq hksqdssevt kyilqykegd ggmwqqqeft
      961 gpplpyaali delkpatryt irviaegpag rsvpsaelvv rtepqrpagp pinlaarals
     1021 sseilitwsp plpelrhgdi qgfnvgyret ssanpsynfs svsgdgeegg aelrltglrp
     1081 ytkytlvvqa ynqvgsgpls eplltqtled vpsmppedvr caaltsqslq vtwepppnth
     1141 sngiiqgykl hyepiladiw rsvdemevrk tsalttvltg lrkytnytiq vlaftrvgdg
     1201 vptiatycqt eedvpgspad ikvvvsspqa lfiswlpple pngiitkynl ytrvvdgree
     1261 lnhgkktlpa tntffeatdl qqhveyqfwv tgstrvgegq ssrvaaqvpt nrvparitsf
     1321 gghvvrpwrg satlacnavg dptrewykgv aeqirtdttr niqilpsgel vlsnlqsqdg
     1381 gnytcqvena qgsdklhytl tvqvppsapv lyvtsstsss illhwkpghs ggapltgytl
     1441 hyrtthgnld elqlsrhats helkgllcgn tyqlyltshn kigsspsspv lsvrtqgqap
     1501 gippaaafls pnsttlvlrl hvwpdngcpi lyfviqyrpi nefhwtlvsn svkmqrrfvv
     1561 tnlqpssvyq lkveahnvag snqaeftfvt ltkegepppp elskrgitsa vpfyadvkvm
     1621 lpliaatial lvavvivalr wrsrylgerm qrpmketqen qqnaetqrer yyatihkval
     1681 qqgagpdkip etaedispya tfqlsegggg slaglgglgg lgsaaeasaa glhpnntllh
     1741 sfmyhehamt egcaspppaa tsmksvssrr rqqrkqqtpg dvesdesesd pdqltssrte
     1801 ssnqldagkl khiravsdfi yhgtsstssd ispmseqksl prrgrsrwhv psrsslrtll
     1861 ppisvaettf vganqgnvvp gngdradrpe lseaecdids lkklklglrs slwsrpstqn
     1921 npssdysiav
//
LOCUS       XP_076546781            1925 aa            linear   INV 03-SEP-2025
DEFINITION  Down syndrome cell adhesion molecule 2 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546781
VERSION     XP_076546781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690666.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1925
                     /product="Down syndrome cell adhesion molecule 2 isoform
                     X5"
                     /calculated_mol_wt=209367
     Region          29..127
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          48..52
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409547"
     Region          61..65
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409547"
     Region          85..89
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409547"
     Region          105..110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409547"
     Region          118..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409547"
     Region          237..314
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          330..417
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          348..352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          421..516
                     /region_name="IgI_4_Dscam"
                     /note="Fourth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20956"
                     /db_xref="CDD:409548"
     Region          421..424
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409548"
     Region          430..434
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409548"
     Region          437..446
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409548"
     Region          451..457
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409548"
     Region          459..462
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409548"
     Region          467..475
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409548"
     Region          479..488
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409548"
     Region          495..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409548"
     Region          506..516
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409548"
     Region          520..607
                     /region_name="IgI_5_Dscam"
                     /note="Fifth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20958"
                     /db_xref="CDD:409550"
     Region          520..522
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409550"
     Region          527..531
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409550"
     Region          534..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409550"
     Region          548..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409550"
     Region          555..557
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409550"
     Region          564..568
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409550"
     Region          571..577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409550"
     Region          585..593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409550"
     Region          597..607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409550"
     Region          610..702
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          628..632
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          668..672
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          682..687
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          695..698
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          705..800
                     /region_name="IgI_7_Dscam"
                     /note="Seventh immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20954"
                     /db_xref="CDD:409546"
     Region          705..709
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409546"
     Region          714..718
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409546"
     Region          721..730
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409546"
     Region          736..742
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409546"
     Region          748..751
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409546"
     Region          759..762
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409546"
     Region          765..771
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409546"
     Region          778..786
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409546"
     Region          791..800
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409546"
     Region          810..900
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          821..825
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          834..838
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          866..870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          880..885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          893..896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          904..997
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(904,970,985)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <966..1310
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(986..987,989..990)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1327..1387
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          1327..1331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1363..1367
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1377..1382
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            order(1401,1463,1478)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          1416..1478
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          1495..1575
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1495,1560,1575)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..1925
                     /gene="Dscam2"
                     /coded_by="XM_076690666.1:452..6229"
                     /db_xref="GeneID:117611830"
ORIGIN      
        1 mlarclicig avaavtsagg hgfdahlrgp sfviepssrv efsnssgawl dctatgsppp
       61 nidwstadgh pvndvsgvrr vlrngtlvll pfpaaafrqd vhsaayrcva snsvgrvlsr
      121 dvqvravvaq aykvdvevig gasrgctavl rcvvpsfvkd lvrvvswlqe psfyiypslq
      181 gdgkfhllpt gellvhslef ndqihgyrcr tmhrltrqvv vssmanvria dhrgvmppvi
      241 lensgvvhva qdestslvcv aqacptpeyr wyaqtgsepm lvlsgprtrl lgsvlaleav
      301 tledsgiyrc saanpggeas aeirlivtap lhvevtppll svhlggnaef rcevsthpqa
      361 gphfvtwykd grqlpgtgrq sellrlngig redrgmyqci vrraegdtaq asaelqlgda
      421 ppmllysfie qtlqpgpavs lkcsaagnpt pqvswaldgf plptngrfvi gqyvtvhgdv
      481 ishvnishvm vedggeysct aenragkvth aarlnvyglp yirlipkvta vagetlrlkc
      541 pvagypieei kweranrelp ddlrqkvlpd gtlvitsvqk kgdagvytcs arnkqghsar
      601 rsgdvavivp pkispfttdr dihlgerttl tcsvtrgdlp ltiswlkdgr amgpservtv
      661 tnmdqynsil mieslspdhn gnyscvarnl aaevshtqrl vvhvpprwiv eptdvsvern
      721 khvalhcqaq gvptptivwk katgsksgey eelreraytk ilsngtlllq hvkedregfy
      781 lcqasngigs gigkvvqlkv nsspyfaaps rlvtvkkgdt atlhcevhgd tpvtvtwlkg
      841 gkielnpstn yrvtvkrevt pdgviaqlqi ssaeasdsga yfcqasnlyg rdqqlvqllv
      901 qeppqppnsl etamvasrsi nvkwqhksqd ssevtkyilq ykegdggmwq qqeftgpplp
      961 yaalidelkp atrytirvia egpagrsvps aelvvrtepq rpagppinla aralssseil
     1021 itwspplpel rhgdiqgfnv gyretssanp synfssvsgd geeggaelrl tglrpytkyt
     1081 lvvqaynqvg sgplsepllt qtledvpsmp pedvrcaalt sqslqvtwep ppnthsngii
     1141 qgyklhyepi ladiwrsvde mevrktsalt tvltglrkyt nytiqvlaft rvgdgvptia
     1201 tycqteedvp gspadikvvv sspqalfisw lpplepngii tkynlytrvv dgreelnhgk
     1261 ktlpatntff eatdlqqhve yqfwvtgstr vgegqssrva aqvptnrvpa ritsfgghvv
     1321 rpwrgsatla cnavgdptre wykgvaeqir tdttrniqil psgelvlsnl qsqdggnytc
     1381 qvenaqgsdk lhytltvqvp psapvlyvts stsssillhw kpghsggapl tgytlhyrtt
     1441 hgnldelqls rhatshelkg llcgntyqly ltshnkigss psspvlsvrt qgqapgippa
     1501 aaflspnstt lvlrlhvwpd ngcpilyfvi qyrpinefhw tlvsnsvkmq rrfvvtnlqp
     1561 ssvyqlkvea hnvagsnqae ftfvtltkeg eppppelskr gitsavpfya dvkvmlplia
     1621 atiallvavv ivalrwrsry lgermqrpmk etqenqqnae tqreryyati hkvalqqgag
     1681 pdkipetaed ispyatfqls eggggslagl gglgglgsaa easaaglhpn ntllhsfmyh
     1741 ehamtegcas pppaatsmks vssrrrqqrk qqtpgdvesd esesdpdqlt ssrtessnql
     1801 dagklkhira vsdfiyhgts stssdispms eqkslprrgr srwhvpsrss lrtllppisv
     1861 aettfvganq gnvvpgngdr adrpelseae cdidslkklk lglrsslwsr pstqnnpssd
     1921 ysiav
//
LOCUS       XP_034196076            1925 aa            linear   INV 03-SEP-2025
DEFINITION  Down syndrome cell adhesion molecule 2 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196076
VERSION     XP_034196076.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340185.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1925
                     /product="Down syndrome cell adhesion molecule 2 isoform
                     X6"
                     /calculated_mol_wt=208928
     Region          29..127
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          48..52
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          61..65
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          85..89
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          105..110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          237..314
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          330..417
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          348..352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          421..516
                     /region_name="IgI_4_Dscam"
                     /note="Fourth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20956"
                     /db_xref="CDD:409548"
     Region          421..424
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409548"
     Region          430..434
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409548"
     Region          437..446
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409548"
     Region          451..457
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409548"
     Region          459..462
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409548"
     Region          467..475
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409548"
     Region          479..488
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409548"
     Region          495..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409548"
     Region          506..516
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409548"
     Region          520..607
                     /region_name="IgI_5_Dscam"
                     /note="Fifth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20958"
                     /db_xref="CDD:409550"
     Region          520..522
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409550"
     Region          527..531
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409550"
     Region          534..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409550"
     Region          548..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409550"
     Region          555..557
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409550"
     Region          564..568
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409550"
     Region          571..577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409550"
     Region          585..593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409550"
     Region          597..607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409550"
     Region          610..707
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          628..632
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          673..677
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          687..692
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          700..703
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          710..805
                     /region_name="IgI_7_Dscam"
                     /note="Seventh immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20954"
                     /db_xref="CDD:409546"
     Region          710..714
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409546"
     Region          719..723
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409546"
     Region          726..735
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409546"
     Region          741..747
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409546"
     Region          753..756
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409546"
     Region          764..767
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409546"
     Region          770..776
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409546"
     Region          783..791
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409546"
     Region          796..805
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409546"
     Region          815..905
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          826..830
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          839..843
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          871..875
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          885..890
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          898..901
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          909..1002
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(909,975,990)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <971..1315
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(991..992,994..995)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1332..1392
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          1332..1336
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1368..1372
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1382..1387
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            order(1406,1468,1483)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          1421..1483
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          1500..1580
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1500,1565,1580)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..1925
                     /gene="Dscam2"
                     /coded_by="XM_034340185.2:452..6229"
                     /db_xref="GeneID:117611830"
ORIGIN      
        1 mlarclicig avaavtsagg hgfdahlrgp sfviepssrv efsnssgawl dctatgsppp
       61 nidwstadgh pvndvsgvrr vlrngtlvll pfpaaafrqd vhsaayrcva snsvgrvlsr
      121 dvqvravvaq aykvdvevig gasrgctavl rcvvpsfvkd lvrvvswlqe psfyiypslq
      181 gdgkfhllpt gellvhslef ndqihgyrcr tmhrltrqvv vssmanvria dhrgvmppvi
      241 lensgvvhva qdestslvcv aqacptpeyr wyaqtgsepm lvlsgprtrl lgsvlaleav
      301 tledsgiyrc saanpggeas aeirlivtap lhvevtppll svhlggnaef rcevsthpqa
      361 gphfvtwykd grqlpgtgrq sellrlngig redrgmyqci vrraegdtaq asaelqlgda
      421 ppmllysfie qtlqpgpavs lkcsaagnpt pqvswaldgf plptngrfvi gqyvtvhgdv
      481 ishvnishvm vedggeysct aenragkvth aarlnvyglp yirlipkvta vagetlrlkc
      541 pvagypieei kweranrelp ddlrqkvlpd gtlvitsvqk kgdagvytcs arnkqghsar
      601 rsgdvavivp piiepftfqe glsegmrtrt vcgvaagdpp ltiswlkdgq tpfplppnla
      661 svanvsqldp yssllsitnl aaehsgdytc vaanpaaevr ytaklqvkvp prwiveptdv
      721 svernkhval hcqaqgvptp tivwkkatgs ksgeyeelre raytkilsng tlllqhvked
      781 regfylcqas ngigsgigkv vqlkvnsspy faapsrlvtv kkgdtatlhc evhgdtpvtv
      841 twlkggkiel npstnyrvtv krevtpdgvi aqlqissaea sdsgayfcqa snlygrdqql
      901 vqllvqeppq ppnsletamv asrsinvkwq hksqdssevt kyilqykegd ggmwqqqeft
      961 gpplpyaali delkpatryt irviaegpag rsvpsaelvv rtepqrpagp pinlaarals
     1021 sseilitwsp plpelrhgdi qgfnvgyret ssanpsynfs svsgdgeegg aelrltglrp
     1081 ytkytlvvqa ynqvgsgpls eplltqtled vpsmppedvr caaltsqslq vtwepppnth
     1141 sngiiqgykl hyepiladiw rsvdemevrk tsalttvltg lrkytnytiq vlaftrvgdg
     1201 vptiatycqt eedvpgspad ikvvvsspqa lfiswlpple pngiitkynl ytrvvdgree
     1261 lnhgkktlpa tntffeatdl qqhveyqfwv tgstrvgegq ssrvaaqvpt nrvparitsf
     1321 gghvvrpwrg satlacnavg dptrewykgv aeqirtdttr niqilpsgel vlsnlqsqdg
     1381 gnytcqvena qgsdklhytl tvqvppsapv lyvtsstsss illhwkpghs ggapltgytl
     1441 hyrtthgnld elqlsrhats helkgllcgn tyqlyltshn kigsspsspv lsvrtqgqap
     1501 gippaaafls pnsttlvlrl hvwpdngcpi lyfviqyrpi nefhwtlvsn svkmqrrfvv
     1561 tnlqpssvyq lkveahnvag snqaeftfvt ltkegelskr gitsavpfya dvkvmlplia
     1621 atiallvavv ivalrwrsry lgermqrpmk etqenqqnae tqreryyati hkvalqqgag
     1681 pdkipetaed ispyatfqls eggggslagl gglgglgsaa easaaglhpn ntllhsfmyh
     1741 ehamtegcas pppaatsmks vssrrrqqrk qqtpgdvesd esesdpdqlt ssrtessnql
     1801 dagklkhira vsdfiyhgts stssdispms eqkslprrgr srwhvpsrss lrtllppisv
     1861 aettfvganq gnvvpgngdr adrpelseae cdidslkklk lglrsslwsr pstqnnpssd
     1921 ysiav
//
LOCUS       XP_034196077            1920 aa            linear   INV 03-SEP-2025
DEFINITION  Down syndrome cell adhesion molecule 2 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196077
VERSION     XP_034196077.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340186.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1920
                     /product="Down syndrome cell adhesion molecule 2 isoform
                     X7"
                     /calculated_mol_wt=208849
     Region          29..127
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          48..52
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          61..65
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          85..89
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          105..110
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          118..122
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          237..314
                     /region_name="Ig_3"
                     /note="Immunoglobulin domain; pfam13927"
                     /db_xref="CDD:464046"
     Region          330..417
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          348..352
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          364..368
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          396..401
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          410..413
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          421..516
                     /region_name="IgI_4_Dscam"
                     /note="Fourth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20956"
                     /db_xref="CDD:409548"
     Region          421..424
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409548"
     Region          430..434
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409548"
     Region          437..446
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409548"
     Region          451..457
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409548"
     Region          459..462
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409548"
     Region          467..475
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409548"
     Region          479..488
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409548"
     Region          495..503
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409548"
     Region          506..516
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409548"
     Region          520..607
                     /region_name="IgI_5_Dscam"
                     /note="Fifth immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20958"
                     /db_xref="CDD:409550"
     Region          520..522
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409550"
     Region          527..531
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409550"
     Region          534..541
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409550"
     Region          548..554
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409550"
     Region          555..557
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409550"
     Region          564..568
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409550"
     Region          571..577
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409550"
     Region          585..593
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409550"
     Region          597..607
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409550"
     Region          610..702
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          628..632
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          642..646
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          668..672
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          682..687
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          695..698
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          705..800
                     /region_name="IgI_7_Dscam"
                     /note="Seventh immunoglobulin domain of the Drosophila
                     melanogaster Dscam protein, and similar domains; a member
                     of the I-set of IgSF domains; cd20954"
                     /db_xref="CDD:409546"
     Region          705..709
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409546"
     Region          714..718
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409546"
     Region          721..730
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409546"
     Region          736..742
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409546"
     Region          748..751
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409546"
     Region          759..762
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409546"
     Region          765..771
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409546"
     Region          778..786
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409546"
     Region          791..800
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409546"
     Region          810..900
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          821..825
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          834..838
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          866..870
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          880..885
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          893..896
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          904..997
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(904,970,985)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          <966..1310
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(986..987,989..990)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1327..1387
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cd00096"
                     /db_xref="CDD:409353"
     Region          1327..1331
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          1363..1367
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          1377..1382
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            order(1401,1463,1478)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Region          1416..1478
                     /region_name="fn3"
                     /note="Fibronectin type III domain; pfam00041"
                     /db_xref="CDD:394996"
     Region          1495..1575
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1495,1560,1575)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     CDS             1..1920
                     /gene="Dscam2"
                     /coded_by="XM_034340186.2:452..6214"
                     /db_xref="GeneID:117611830"
ORIGIN      
        1 mlarclicig avaavtsagg hgfdahlrgp sfviepssrv efsnssgawl dctatgsppp
       61 nidwstadgh pvndvsgvrr vlrngtlvll pfpaaafrqd vhsaayrcva snsvgrvlsr
      121 dvqvravvaq aykvdvevig gasrgctavl rcvvpsfvkd lvrvvswlqe psfyiypslq
      181 gdgkfhllpt gellvhslef ndqihgyrcr tmhrltrqvv vssmanvria dhrgvmppvi
      241 lensgvvhva qdestslvcv aqacptpeyr wyaqtgsepm lvlsgprtrl lgsvlaleav
      301 tledsgiyrc saanpggeas aeirlivtap lhvevtppll svhlggnaef rcevsthpqa
      361 gphfvtwykd grqlpgtgrq sellrlngig redrgmyqci vrraegdtaq asaelqlgda
      421 ppmllysfie qtlqpgpavs lkcsaagnpt pqvswaldgf plptngrfvi gqyvtvhgdv
      481 ishvnishvm vedggeysct aenragkvth aarlnvyglp yirlipkvta vagetlrlkc
      541 pvagypieei kweranrelp ddlrqkvlpd gtlvitsvqk kgdagvytcs arnkqghsar
      601 rsgdvavivp pkispfttdr dihlgerttl tcsvtrgdlp ltiswlkdgr amgpservtv
      661 tnmdqynsil mieslspdhn gnyscvarnl aaevshtqrl vvhvpprwiv eptdvsvern
      721 khvalhcqaq gvptptivwk katgsksgey eelreraytk ilsngtlllq hvkedregfy
      781 lcqasngigs gigkvvqlkv nsspyfaaps rlvtvkkgdt atlhcevhgd tpvtvtwlkg
      841 gkielnpstn yrvtvkrevt pdgviaqlqi ssaeasdsga yfcqasnlyg rdqqlvqllv
      901 qeppqppnsl etamvasrsi nvkwqhksqd ssevtkyilq ykegdggmwq qqeftgpplp
      961 yaalidelkp atrytirvia egpagrsvps aelvvrtepq rpagppinla aralssseil
     1021 itwspplpel rhgdiqgfnv gyretssanp synfssvsgd geeggaelrl tglrpytkyt
     1081 lvvqaynqvg sgplsepllt qtledvpsmp pedvrcaalt sqslqvtwep ppnthsngii
     1141 qgyklhyepi ladiwrsvde mevrktsalt tvltglrkyt nytiqvlaft rvgdgvptia
     1201 tycqteedvp gspadikvvv sspqalfisw lpplepngii tkynlytrvv dgreelnhgk
     1261 ktlpatntff eatdlqqhve yqfwvtgstr vgegqssrva aqvptnrvpa ritsfgghvv
     1321 rpwrgsatla cnavgdptre wykgvaeqir tdttrniqil psgelvlsnl qsqdggnytc
     1381 qvenaqgsdk lhytltvqvp psapvlyvts stsssillhw kpghsggapl tgytlhyrtt
     1441 hgnldelqls rhatshelkg llcgntyqly ltshnkigss psspvlsvrt qgqapgippa
     1501 aaflspnstt lvlrlhvwpd ngcpilyfvi qyrpinefhw tlvsnsvkmq rrfvvtnlqp
     1561 ssvyqlkvea hnvagsnqae ftfvtltkeg elskrgitsa vpfyadvkvm lpliaatial
     1621 lvavvivalr wrsrylgerm qrpmketqen qqnaetqrer yyatihkval qqgagpdkip
     1681 etaedispya tfqlsegggg slaglgglgg lgsaaeasaa glhpnntllh sfmyhehamt
     1741 egcaspppaa tsmksvssrr rqqrkqqtpg dvesdesesd pdqltssrte ssnqldagkl
     1801 khiravsdfi yhgtsstssd ispmseqksl prrgrsrwhv psrsslrtll ppisvaettf
     1861 vganqgnvvp gngdradrpe lseaecdids lkklklglrs slwsrpstqn npssdysiav
//
LOCUS       XP_076546782            1268 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546782
VERSION     XP_076546782.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690667.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1268
                     /product="JNK-interacting protein syd isoform X1"
                     /calculated_mol_wt=140668
     Region          30..203
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          386..447
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          896..>1120
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1268
                     /gene="syd"
                     /coded_by="XM_076690667.1:152..3958"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhgtk atgslrpslh ktslyivrle ekeaemkrey
      181 trlherytel fkthvdymer tkmlvgster lenatsgrgp srlpslglah msrssgplsy
      241 gfqsleasin aedvqddipp naanlrteml dnsseaaiet sdksqltdkp akankttais
      301 rhespeteap pllvtptspt veklattpsg rsrtereqrs gntlyqelsf qdadalgemd
      361 egaditgswv hpgeyassvn dnffgmgkev enlimennel latknalniv kddlivkvde
      421 ltseqeilre evrglqqare rlkqrvaale eelkkvkeea eaaakaaksd deedvplaqr
      481 krftrvemar vlmernqyke rfmelqeavr wtemiraskt dpssisggkg svwkffsslf
      541 tgpadrgtlv rgahtlphir ysaptnqvvp assldamrrr tlkgrheifd qgdtiasekl
      601 varranerre qyrqvrahvr kedgrlqayg wslpgkpsap vrqpvpvpvy crplqesepg
      661 mkiwcgagvn lsggktrdgg cmiggsvfya aeaqevssnt kveaedaveh ldkeiqenen
      721 qrveaeqleq qlsslvwict stqkmskvtv idannpagvl evfnvcqghl lciasvpgak
      781 esdyiqgvne epvqttngvn dndgheenan veqinqknkq etqapsdksk nesdsvseeq
      841 nnestesdnt nqnittepqs lesvdsetmn lgkvyfvkvn lesppsrlne kddtneenqn
      901 deveegtpie kmssiqptmw lgaqngavfv hsavakwsvc lhsvklkdaa laivhvqgrv
      961 lvaladgtva lfrrgadgqw dlsqyhvitl gspqhsircm aavsgrtvwc gyrnkihvid
     1021 pvlmtvectv dahprresqv rqlawlgegv wvsirldstl rlyhahtyqh lqdvdiepyv
     1081 skmlgtgklg fsfvritall issnrlwigt gngviisvpl aedvyvvgag gsmavsrvqa
     1141 asgkteapgv gvrifasdrs vtrgsfipyc smahaqlsfh ghrdavkmfv avpghggqsa
     1201 vsdgsqpaml vlsggegyid frvgdgedte esmersnsav aanaevhgeq shlivwqvqc
     1261 plpvpmng
//
LOCUS       XP_076546783            1264 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546783
VERSION     XP_076546783.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690668.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1264
                     /product="JNK-interacting protein syd isoform X2"
                     /calculated_mol_wt=140146
     Region          26..199
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          382..443
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          892..>1116
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1264
                     /gene="syd"
                     /coded_by="XM_076690668.1:310..4104"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 msqvemdqet vygthedshv vmsekvqsla gsiyqefekm iarydedvvk dlmpllvnvl
       61 ecldisyten qerevelell redneqlvtq yerekqlrka sdqklfeled vaederkelv
      121 skidslesiv rmlelktkns hdhgtkatgs lrpslhktsl yivrleekea emkreytrlh
      181 erytelfkth vdymertkml vgsterlena tsgrgpsrlp slglahmsrs sgplsygfqs
      241 leasinaedv qddippnaan lrtemldnss eaaietsdks qltdkpakan kttaisrhes
      301 peteappllv tptsptvekl attpsgrsrt ereqrsgntl yqelsfqdad algemdegad
      361 itgswvhpge yassvndnff gmgkevenli mennellatk nalnivkddl ivkvdeltse
      421 qeilreevrg lqqarerlkq rvaaleeelk kvkeeaeaaa kaaksddeed vplaqrkrft
      481 rvemarvlme rnqykerfme lqeavrwtem irasktdpss isggkgsvwk ffsslftgpa
      541 drgtlvrgah tlphirysap tnqvvpassl damrrrtlkg rheifdqgdt iaseklvarr
      601 anerreqyrq vrahvrkedg rlqaygwslp gkpsapvrqp vpvpvycrpl qesepgmkiw
      661 cgagvnlsgg ktrdggcmig gsvfyaaeaq evssntkvea edavehldke iqenenqrve
      721 aeqleqqlss lvwictstqk mskvtvidan npagvlevfn vcqghllcia svpgakesdy
      781 iqgvneepvq ttngvndndg heenanveqi nqknkqetqa psdksknesd svseeqnnes
      841 tesdntnqni ttepqslesv dsetmnlgkv yfvkvnlesp psrlnekddt neenqndeve
      901 egtpiekmss iqptmwlgaq ngavfvhsav akwsvclhsv klkdaalaiv hvqgrvlval
      961 adgtvalfrr gadgqwdlsq yhvitlgspq hsircmaavs grtvwcgyrn kihvidpvlm
     1021 tvectvdahp rresqvrqla wlgegvwvsi rldstlrlyh ahtyqhlqdv diepyvskml
     1081 gtgklgfsfv ritallissn rlwigtgngv iisvplaedv yvvgaggsma vsrvqaasgk
     1141 teapgvgvri fasdrsvtrg sfipycsmah aqlsfhghrd avkmfvavpg hggqsavsdg
     1201 sqpamlvlsg gegyidfrvg dgedteesme rsnsavaana evhgeqshli vwqvqcplpv
     1261 pmng
//
LOCUS       XP_076546784            1264 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546784
VERSION     XP_076546784.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690669.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1264
                     /product="JNK-interacting protein syd isoform X2"
                     /calculated_mol_wt=140146
     Region          26..199
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          382..443
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          892..>1116
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1264
                     /gene="syd"
                     /coded_by="XM_076690669.1:163..3957"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 msqvemdqet vygthedshv vmsekvqsla gsiyqefekm iarydedvvk dlmpllvnvl
       61 ecldisyten qerevelell redneqlvtq yerekqlrka sdqklfeled vaederkelv
      121 skidslesiv rmlelktkns hdhgtkatgs lrpslhktsl yivrleekea emkreytrlh
      181 erytelfkth vdymertkml vgsterlena tsgrgpsrlp slglahmsrs sgplsygfqs
      241 leasinaedv qddippnaan lrtemldnss eaaietsdks qltdkpakan kttaisrhes
      301 peteappllv tptsptvekl attpsgrsrt ereqrsgntl yqelsfqdad algemdegad
      361 itgswvhpge yassvndnff gmgkevenli mennellatk nalnivkddl ivkvdeltse
      421 qeilreevrg lqqarerlkq rvaaleeelk kvkeeaeaaa kaaksddeed vplaqrkrft
      481 rvemarvlme rnqykerfme lqeavrwtem irasktdpss isggkgsvwk ffsslftgpa
      541 drgtlvrgah tlphirysap tnqvvpassl damrrrtlkg rheifdqgdt iaseklvarr
      601 anerreqyrq vrahvrkedg rlqaygwslp gkpsapvrqp vpvpvycrpl qesepgmkiw
      661 cgagvnlsgg ktrdggcmig gsvfyaaeaq evssntkvea edavehldke iqenenqrve
      721 aeqleqqlss lvwictstqk mskvtvidan npagvlevfn vcqghllcia svpgakesdy
      781 iqgvneepvq ttngvndndg heenanveqi nqknkqetqa psdksknesd svseeqnnes
      841 tesdntnqni ttepqslesv dsetmnlgkv yfvkvnlesp psrlnekddt neenqndeve
      901 egtpiekmss iqptmwlgaq ngavfvhsav akwsvclhsv klkdaalaiv hvqgrvlval
      961 adgtvalfrr gadgqwdlsq yhvitlgspq hsircmaavs grtvwcgyrn kihvidpvlm
     1021 tvectvdahp rresqvrqla wlgegvwvsi rldstlrlyh ahtyqhlqdv diepyvskml
     1081 gtgklgfsfv ritallissn rlwigtgngv iisvplaedv yvvgaggsma vsrvqaasgk
     1141 teapgvgvri fasdrsvtrg sfipycsmah aqlsfhghrd avkmfvavpg hggqsavsdg
     1201 sqpamlvlsg gegyidfrvg dgedteesme rsnsavaana evhgeqshli vwqvqcplpv
     1261 pmng
//
LOCUS       XP_076546785            1264 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546785
VERSION     XP_076546785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690670.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1264
                     /product="JNK-interacting protein syd isoform X2"
                     /calculated_mol_wt=140146
     Region          26..199
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          382..443
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          892..>1116
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1264
                     /gene="syd"
                     /coded_by="XM_076690670.1:223..4017"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 msqvemdqet vygthedshv vmsekvqsla gsiyqefekm iarydedvvk dlmpllvnvl
       61 ecldisyten qerevelell redneqlvtq yerekqlrka sdqklfeled vaederkelv
      121 skidslesiv rmlelktkns hdhgtkatgs lrpslhktsl yivrleekea emkreytrlh
      181 erytelfkth vdymertkml vgsterlena tsgrgpsrlp slglahmsrs sgplsygfqs
      241 leasinaedv qddippnaan lrtemldnss eaaietsdks qltdkpakan kttaisrhes
      301 peteappllv tptsptvekl attpsgrsrt ereqrsgntl yqelsfqdad algemdegad
      361 itgswvhpge yassvndnff gmgkevenli mennellatk nalnivkddl ivkvdeltse
      421 qeilreevrg lqqarerlkq rvaaleeelk kvkeeaeaaa kaaksddeed vplaqrkrft
      481 rvemarvlme rnqykerfme lqeavrwtem irasktdpss isggkgsvwk ffsslftgpa
      541 drgtlvrgah tlphirysap tnqvvpassl damrrrtlkg rheifdqgdt iaseklvarr
      601 anerreqyrq vrahvrkedg rlqaygwslp gkpsapvrqp vpvpvycrpl qesepgmkiw
      661 cgagvnlsgg ktrdggcmig gsvfyaaeaq evssntkvea edavehldke iqenenqrve
      721 aeqleqqlss lvwictstqk mskvtvidan npagvlevfn vcqghllcia svpgakesdy
      781 iqgvneepvq ttngvndndg heenanveqi nqknkqetqa psdksknesd svseeqnnes
      841 tesdntnqni ttepqslesv dsetmnlgkv yfvkvnlesp psrlnekddt neenqndeve
      901 egtpiekmss iqptmwlgaq ngavfvhsav akwsvclhsv klkdaalaiv hvqgrvlval
      961 adgtvalfrr gadgqwdlsq yhvitlgspq hsircmaavs grtvwcgyrn kihvidpvlm
     1021 tvectvdahp rresqvrqla wlgegvwvsi rldstlrlyh ahtyqhlqdv diepyvskml
     1081 gtgklgfsfv ritallissn rlwigtgngv iisvplaedv yvvgaggsma vsrvqaasgk
     1141 teapgvgvri fasdrsvtrg sfipycsmah aqlsfhghrd avkmfvavpg hggqsavsdg
     1201 sqpamlvlsg gegyidfrvg dgedteesme rsnsavaana evhgeqshli vwqvqcplpv
     1261 pmng
//
LOCUS       XP_076546786            1263 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546786
VERSION     XP_076546786.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690671.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1263
                     /product="JNK-interacting protein syd isoform X3"
                     /calculated_mol_wt=140092
     Region          30..203
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          386..447
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          896..>1120
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1263
                     /gene="syd"
                     /coded_by="XM_076690671.1:151..3942"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhgtk atgslrpslh ktslyivrle ekeaemkrey
      181 trlherytel fkthvdymer tkmlvgster lenatsgrgp srlpslglah msrssgplsy
      241 gfqsleasin aedvqddipp naanlrteml dnsseaaiet sdksqltdkp akankttais
      301 rhespeteap pllvtptspt veklattpsg rsrtereqrs gntlyqelsf qdadalgemd
      361 egaditgswv hpgeyassvn dnffgmgkev enlimennel latknalniv kddlivkvde
      421 ltseqeilre evrglqqare rlkqrvaale eelkkvkeea eaaakaaksd deedvplaqr
      481 krftrvemar vlmernqyke rfmelqeavr wtemiraskt dpssisggkg svwkffsslf
      541 tgpadrgtlv rgahtlphir ysaptnqvvp assldamrrr tlkgrheifd qgdtiasekl
      601 varranerre qyrqvrahvr kedgrlqayg wslpgkpsap vrqpvpvpvy crplqesepg
      661 mkiwcgagvn lsggktrdgg cmiggsvfya aeaqevssnt kveaedaveh ldkeiqenen
      721 qrveaeqleq qlsslvwict stqkmskvtv idannpagvl evfnvcqghl lciasvpgak
      781 esdyiqgvne epvqttngvn dndgheenan veqinqknkq etqapsdksk nesdsvseeq
      841 nnestesdnt nqnittepqs lesvdsetmn lgkvyfvkvn lesppsrlne kddtneenqn
      901 deveegtpie kmssiqptmw lgaqngavfv hsavakwsvc lhsvklkdaa laivhvqgrv
      961 lvaladgtva lfrrgadgqw dlsqyhvitl gspqhsircm aavsgrtvwc gyrnkihvid
     1021 pvlmtvectv dahprresqv rqlawlgegv wvsirldstl rlyhahtyqh lqdvdiepyv
     1081 skmlgtgklg fsfvritall issnrlwigt gngviisvpl aegaggsmav srvqaasgkt
     1141 eapgvgvrif asdrsvtrgs fipycsmaha qlsfhghrda vkmfvavpgh ggqsavsdgs
     1201 qpamlvlsgg egyidfrvgd gedteesmer snsavaanae vhgeqshliv wqvqcplpvp
     1261 mng
//
LOCUS       XP_076546787            1262 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546787
VERSION     XP_076546787.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690672.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1262
                     /product="JNK-interacting protein syd isoform X4"
                     /calculated_mol_wt=139931
     Region          30..203
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          380..441
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          890..>1114
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1262
                     /gene="syd"
                     /coded_by="XM_076690672.1:153..3941"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhgtk atgslrpslh ktslyivrle ekeaemkrey
      181 trlherytel fkthvdymer tkmlvgster lenatsgrgp srlpslglah msrssgplsy
      241 gfqsleasin aedvqddipp naanlrteml dnsseaaiet sdksqltdkp akankttais
      301 rhespeteap pllvtptspt veklattpsg rsrtereqrs gntlyqelsf qdadalgemd
      361 egaditgswv hpgeyassgm gkevenlime nnellatkna lnivkddliv kvdeltseqe
      421 ilreevrglq qarerlkqrv aaleeelkkv keeaeaaaka aksddeedvp laqrkrftrv
      481 emarvlmern qykerfmelq eavrwtemir asktdpssis ggkgsvwkff sslftgpadr
      541 gtlvrgahtl phirysaptn qvvpasslda mrrrtlkgrh eifdqgdtia seklvarran
      601 erreqyrqvr ahvrkedgrl qaygwslpgk psapvrqpvp vpvycrplqe sepgmkiwcg
      661 agvnlsggkt rdggcmiggs vfyaaeaqev ssntkveaed avehldkeiq enenqrveae
      721 qleqqlsslv wictstqkms kvtvidannp agvlevfnvc qghllciasv pgakesdyiq
      781 gvneepvqtt ngvndndghe enanveqinq knkqetqaps dksknesdsv seeqnneste
      841 sdntnqnitt epqslesvds etmnlgkvyf vkvnlespps rlnekddtne enqndeveeg
      901 tpiekmssiq ptmwlgaqng avfvhsavak wsvclhsvkl kdaalaivhv qgrvlvalad
      961 gtvalfrrga dgqwdlsqyh vitlgspqhs ircmaavsgr tvwcgyrnki hvidpvlmtv
     1021 ectvdahprr esqvrqlawl gegvwvsirl dstlrlyhah tyqhlqdvdi epyvskmlgt
     1081 gklgfsfvri tallissnrl wigtgngvii svplaedvyv vgaggsmavs rvqaasgkte
     1141 apgvgvrifa sdrsvtrgsf ipycsmahaq lsfhghrdav kmfvavpghg gqsavsdgsq
     1201 pamlvlsgge gyidfrvgdg edteesmers nsavaanaev hgeqshlivw qvqcplpvpm
     1261 ng
//
LOCUS       XP_034196082            1250 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034196082
VERSION     XP_034196082.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196082.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1250
                     /product="JNK-interacting protein syd isoform X5"
                     /calculated_mol_wt=138755
     Region          30..185
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          368..429
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          878..>1102
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1250
                     /gene="syd"
                     /coded_by="XM_034340191.2:150..3902"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhvvr leekeaemkr eytrlheryt elfkthvdym
      181 ertkmlvgst erlenatsgr gpsrlpslgl ahmsrssgpl sygfqsleas inaedvqddi
      241 ppnaanlrte mldnsseaai etsdksqltd kpakanktta isrhespete appllvtpts
      301 ptveklattp sgrsrtereq rsgntlyqel sfqdadalge mdegaditgs wvhpgeyass
      361 vndnffgmgk evenlimenn ellatknaln ivkddlivkv deltseqeil reevrglqqa
      421 rerlkqrvaa leeelkkvke eaeaaakaak sddeedvpla qrkrftrvem arvlmernqy
      481 kerfmelqea vrwtemiras ktdpssisgg kgsvwkffss lftgpadrgt lvrgahtlph
      541 irysaptnqv vpassldamr rrtlkgrhei fdqgdtiase klvarraner reqyrqvrah
      601 vrkedgrlqa ygwslpgkps apvrqpvpvp vycrplqese pgmkiwcgag vnlsggktrd
      661 ggcmiggsvf yaaeaqevss ntkveaedav ehldkeiqen enqrveaeql eqqlsslvwi
      721 ctstqkmskv tvidannpag vlevfnvcqg hllciasvpg akesdyiqgv neepvqttng
      781 vndndgheen anveqinqkn kqetqapsdk sknesdsvse eqnnestesd ntnqnittep
      841 qslesvdset mnlgkvyfvk vnlesppsrl nekddtneen qndeveegtp iekmssiqpt
      901 mwlgaqngav fvhsavakws vclhsvklkd aalaivhvqg rvlvaladgt valfrrgadg
      961 qwdlsqyhvi tlgspqhsir cmaavsgrtv wcgyrnkihv idpvlmtvec tvdahprres
     1021 qvrqlawlge gvwvsirlds tlrlyhahty qhlqdvdiep yvskmlgtgk lgfsfvrita
     1081 llissnrlwi gtgngviisv plaedvyvvg aggsmavsrv qaasgkteap gvgvrifasd
     1141 rsvtrgsfip ycsmahaqls fhghrdavkm fvavpghggq savsdgsqpa mlvlsggegy
     1201 idfrvgdged teesmersns avaanaevhg eqshlivwqv qcplpvpmng
//
LOCUS       XP_076546788            1250 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076546788
VERSION     XP_076546788.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690673.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1250
                     /product="JNK-interacting protein syd isoform X6"
                     /calculated_mol_wt=138990
     Region          30..203
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          386..447
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          896..>1120
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1250
                     /gene="syd"
                     /coded_by="XM_076690673.1:153..3905"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhgtk atgslrpslh ktslyivrle ekeaemkrey
      181 trlherytel fkthvdymer tkmlvgster lenatsgrgp srlpslglah msrssgplsy
      241 gfqsleasin aedvqddipp naanlrteml dnsseaaiet sdksqltdkp akankttais
      301 rhespeteap pllvtptspt veklattpsg rsrtereqrs gntlyqelsf qdadalgemd
      361 egaditgswv hpgeyassvn dnffgmgkev enlimennel latknalniv kddlivkvde
      421 ltseqeilre evrglqqare rlkqrvaale eelkkvkeea eaaakaaksd deedvplaqr
      481 krftrvemar vlmernqyke rfmelqeavr wtemiraskt dpssisggkg svwkffsslf
      541 tgpadrgtlv rgahtlphir ysaptnqvvp assldamrrr tlkgrheifd qgdtiasekl
      601 varranerre qyrqvrahvr kedgrlqayg wslpgkpsap vrqpvpvpvy crplqesepg
      661 mkiwcgagvn lsggktrdgg cmiggsvfya aeaqevssnt kveaedaveh ldkeiqenen
      721 qrveaeqleq qlsslvwict stqkmskvtv idannpagvl evfnvcqghl lciasvpgak
      781 esdyiqgvne epvqttngvn dndgheenan veqinqknkq etqapsdksk nesdsvseeq
      841 nnestesdnt nqnittepqs lesvdsetmn lgkvyfvkvn lesppsrlne kddtneenqn
      901 deveegtpie kmssiqptmw lgaqngavfv hsavakwsvc lhsvklkdaa laivhvqgrv
      961 lvaladgtva lfrrgadgqw dlsqyhvitl gspqhsircm aavsgrtvwc gyrnkihvid
     1021 pvlmtvectv dahprresqv rqlawlgegv wvsirldstl rlyhahtyqh lqdvdiepyv
     1081 skmlgtgklg fsfvritall issnrlwigt gngviisvpl aedvyvvgag gsmavsrvqa
     1141 asgkteapgv gvrifasdrs vtrgsfipyc smahaqlsfh ghrdavkmfv avpghggqsa
     1201 vsdgsqpaml vlsggegyid frvadeaede sdvathlivw qlvryapree
//
LOCUS       XP_076546789            1249 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076546789
VERSION     XP_076546789.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690674.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1249
                     /product="JNK-interacting protein syd isoform X7"
                     /calculated_mol_wt=138919
     Region          30..203
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          386..447
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          896..>1120
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1249
                     /gene="syd"
                     /coded_by="XM_076690674.1:153..3902"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhgtk atgslrpslh ktslyivrle ekeaemkrey
      181 trlherytel fkthvdymer tkmlvgster lenatsgrgp srlpslglah msrssgplsy
      241 gfqsleasin aedvqddipp naanlrteml dnsseaaiet sdksqltdkp akankttais
      301 rhespeteap pllvtptspt veklattpsg rsrtereqrs gntlyqelsf qdadalgemd
      361 egaditgswv hpgeyassvn dnffgmgkev enlimennel latknalniv kddlivkvde
      421 ltseqeilre evrglqqare rlkqrvaale eelkkvkeea eaaakaaksd deedvplaqr
      481 krftrvemar vlmernqyke rfmelqeavr wtemiraskt dpssisggkg svwkffsslf
      541 tgpadrgtlv rgahtlphir ysaptnqvvp assldamrrr tlkgrheifd qgdtiasekl
      601 varranerre qyrqvrahvr kedgrlqayg wslpgkpsap vrqpvpvpvy crplqesepg
      661 mkiwcgagvn lsggktrdgg cmiggsvfya aeaqevssnt kveaedaveh ldkeiqenen
      721 qrveaeqleq qlsslvwict stqkmskvtv idannpagvl evfnvcqghl lciasvpgak
      781 esdyiqgvne epvqttngvn dndgheenan veqinqknkq etqapsdksk nesdsvseeq
      841 nnestesdnt nqnittepqs lesvdsetmn lgkvyfvkvn lesppsrlne kddtneenqn
      901 deveegtpie kmssiqptmw lgaqngavfv hsavakwsvc lhsvklkdaa laivhvqgrv
      961 lvaladgtva lfrrgadgqw dlsqyhvitl gspqhsircm aavsgrtvwc gyrnkihvid
     1021 pvlmtvectv dahprresqv rqlawlgegv wvsirldstl rlyhahtyqh lqdvdiepyv
     1081 skmlgtgklg fsfvritall issnrlwigt gngviisvpl aedvyvvgag gsmavsrvqa
     1141 asgkteapgv gvrifasdrs vtrgsfipyc smahaqlsfh ghrdavkmfv avpghggqsa
     1201 vsdgsqpaml vlsggegyid frvdeaedes dvathlivwq lvryapree
//
LOCUS       XP_034196087            1245 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034196087
VERSION     XP_034196087.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340196.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196087.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="JNK-interacting protein syd isoform X8"
                     /calculated_mol_wt=138179
     Region          30..185
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          368..429
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          878..>1102
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1245
                     /gene="syd"
                     /coded_by="XM_034340196.2:149..3886"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhvvr leekeaemkr eytrlheryt elfkthvdym
      181 ertkmlvgst erlenatsgr gpsrlpslgl ahmsrssgpl sygfqsleas inaedvqddi
      241 ppnaanlrte mldnsseaai etsdksqltd kpakanktta isrhespete appllvtpts
      301 ptveklattp sgrsrtereq rsgntlyqel sfqdadalge mdegaditgs wvhpgeyass
      361 vndnffgmgk evenlimenn ellatknaln ivkddlivkv deltseqeil reevrglqqa
      421 rerlkqrvaa leeelkkvke eaeaaakaak sddeedvpla qrkrftrvem arvlmernqy
      481 kerfmelqea vrwtemiras ktdpssisgg kgsvwkffss lftgpadrgt lvrgahtlph
      541 irysaptnqv vpassldamr rrtlkgrhei fdqgdtiase klvarraner reqyrqvrah
      601 vrkedgrlqa ygwslpgkps apvrqpvpvp vycrplqese pgmkiwcgag vnlsggktrd
      661 ggcmiggsvf yaaeaqevss ntkveaedav ehldkeiqen enqrveaeql eqqlsslvwi
      721 ctstqkmskv tvidannpag vlevfnvcqg hllciasvpg akesdyiqgv neepvqttng
      781 vndndgheen anveqinqkn kqetqapsdk sknesdsvse eqnnestesd ntnqnittep
      841 qslesvdset mnlgkvyfvk vnlesppsrl nekddtneen qndeveegtp iekmssiqpt
      901 mwlgaqngav fvhsavakws vclhsvklkd aalaivhvqg rvlvaladgt valfrrgadg
      961 qwdlsqyhvi tlgspqhsir cmaavsgrtv wcgyrnkihv idpvlmtvec tvdahprres
     1021 qvrqlawlge gvwvsirlds tlrlyhahty qhlqdvdiep yvskmlgtgk lgfsfvrita
     1081 llissnrlwi gtgngviisv plaegaggsm avsrvqaasg kteapgvgvr ifasdrsvtr
     1141 gsfipycsma haqlsfhghr davkmfvavp ghggqsavsd gsqpamlvls ggegyidfrv
     1201 gdgedteesm ersnsavaan aevhgeqshl ivwqvqcplp vpmng
//
LOCUS       XP_076546790            1244 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X9 [Osmia lignaria lignaria].
ACCESSION   XP_076546790
VERSION     XP_076546790.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690675.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1244
                     /product="JNK-interacting protein syd isoform X9"
                     /calculated_mol_wt=138245
     Region          30..203
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          386..447
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          896..>1120
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1244
                     /gene="syd"
                     /coded_by="XM_076690675.1:153..3887"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhgtk atgslrpslh ktslyivrle ekeaemkrey
      181 trlherytel fkthvdymer tkmlvgster lenatsgrgp srlpslglah msrssgplsy
      241 gfqsleasin aedvqddipp naanlrteml dnsseaaiet sdksqltdkp akankttais
      301 rhespeteap pllvtptspt veklattpsg rsrtereqrs gntlyqelsf qdadalgemd
      361 egaditgswv hpgeyassvn dnffgmgkev enlimennel latknalniv kddlivkvde
      421 ltseqeilre evrglqqare rlkqrvaale eelkkvkeea eaaakaaksd deedvplaqr
      481 krftrvemar vlmernqyke rfmelqeavr wtemiraskt dpssisggkg svwkffsslf
      541 tgpadrgtlv rgahtlphir ysaptnqvvp assldamrrr tlkgrheifd qgdtiasekl
      601 varranerre qyrqvrahvr kedgrlqayg wslpgkpsap vrqpvpvpvy crplqesepg
      661 mkiwcgagvn lsggktrdgg cmiggsvfya aeaqevssnt kveaedaveh ldkeiqenen
      721 qrveaeqleq qlsslvwict stqkmskvtv idannpagvl evfnvcqghl lciasvpgak
      781 esdyiqgvne epvqttngvn dndgheenan veqinqknkq etqapsdksk nesdsvseeq
      841 nnestesdnt nqnittepqs lesvdsetmn lgkvyfvkvn lesppsrlne kddtneenqn
      901 deveegtpie kmssiqptmw lgaqngavfv hsavakwsvc lhsvklkdaa laivhvqgrv
      961 lvaladgtva lfrrgadgqw dlsqyhvitl gspqhsircm aavsgrtvwc gyrnkihvid
     1021 pvlmtvectv dahprresqv rqlawlgegv wvsirldstl rlyhahtyqh lqdvdiepyv
     1081 skmlgtgklg fsfvritall issnrlwigt gngviisvpl aedvyvvgag gsmavsrvqa
     1141 asgkteapgv gvrifasdrs vtrgsfipyc smahaqlsfh ghrdavkmfv avpghggqsa
     1201 vsdgsqpaml vlsggegyid frvadeaede sdvathlivw qlvr
//
LOCUS       XP_076546791            1243 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X10 [Osmia lignaria lignaria].
ACCESSION   XP_076546791
VERSION     XP_076546791.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690676.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1243
                     /product="JNK-interacting protein syd isoform X10"
                     /calculated_mol_wt=138173
     Region          30..203
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          386..447
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          896..>1120
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1243
                     /gene="syd"
                     /coded_by="XM_076690676.1:153..3884"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhgtk atgslrpslh ktslyivrle ekeaemkrey
      181 trlherytel fkthvdymer tkmlvgster lenatsgrgp srlpslglah msrssgplsy
      241 gfqsleasin aedvqddipp naanlrteml dnsseaaiet sdksqltdkp akankttais
      301 rhespeteap pllvtptspt veklattpsg rsrtereqrs gntlyqelsf qdadalgemd
      361 egaditgswv hpgeyassvn dnffgmgkev enlimennel latknalniv kddlivkvde
      421 ltseqeilre evrglqqare rlkqrvaale eelkkvkeea eaaakaaksd deedvplaqr
      481 krftrvemar vlmernqyke rfmelqeavr wtemiraskt dpssisggkg svwkffsslf
      541 tgpadrgtlv rgahtlphir ysaptnqvvp assldamrrr tlkgrheifd qgdtiasekl
      601 varranerre qyrqvrahvr kedgrlqayg wslpgkpsap vrqpvpvpvy crplqesepg
      661 mkiwcgagvn lsggktrdgg cmiggsvfya aeaqevssnt kveaedaveh ldkeiqenen
      721 qrveaeqleq qlsslvwict stqkmskvtv idannpagvl evfnvcqghl lciasvpgak
      781 esdyiqgvne epvqttngvn dndgheenan veqinqknkq etqapsdksk nesdsvseeq
      841 nnestesdnt nqnittepqs lesvdsetmn lgkvyfvkvn lesppsrlne kddtneenqn
      901 deveegtpie kmssiqptmw lgaqngavfv hsavakwsvc lhsvklkdaa laivhvqgrv
      961 lvaladgtva lfrrgadgqw dlsqyhvitl gspqhsircm aavsgrtvwc gyrnkihvid
     1021 pvlmtvectv dahprresqv rqlawlgegv wvsirldstl rlyhahtyqh lqdvdiepyv
     1081 skmlgtgklg fsfvritall issnrlwigt gngviisvpl aedvyvvgag gsmavsrvqa
     1141 asgkteapgv gvrifasdrs vtrgsfipyc smahaqlsfh ghrdavkmfv avpghggqsa
     1201 vsdgsqpaml vlsggegyid frvdeaedes dvathlivwq lvr
//
LOCUS       XP_034196088            1244 aa            linear   INV 03-SEP-2025
DEFINITION  JNK-interacting protein syd isoform X11 [Osmia lignaria lignaria].
ACCESSION   XP_034196088
VERSION     XP_034196088.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340197.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196088.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1244
                     /product="JNK-interacting protein syd isoform X11"
                     /calculated_mol_wt=138018
     Region          30..185
                     /region_name="Jnk-SapK_ap_N"
                     /note="JNK_SAPK-associated protein-1; pfam09744"
                     /db_xref="CDD:462875"
     Region          362..423
                     /region_name="JIP_LZII"
                     /note="JNK-interacting protein leucine zipper II;
                     pfam16471"
                     /db_xref="CDD:465127"
     Region          872..>1096
                     /region_name="WD40_2"
                     /note="WD40 repeated domain; pfam19056"
                     /db_xref="CDD:465964"
     CDS             1..1244
                     /gene="syd"
                     /coded_by="XM_034340197.2:150..3884"
                     /db_xref="GeneID:117611831"
ORIGIN      
        1 mvykmsqvem dqetvygthe dshvvmsekv qslagsiyqe fekmiaryde dvvkdlmpll
       61 vnvlecldis ytenqereve lellredneq lvtqyerekq lrkasdqklf eledvaeder
      121 kelvskidsl esivrmlelk tknshdhvvr leekeaemkr eytrlheryt elfkthvdym
      181 ertkmlvgst erlenatsgr gpsrlpslgl ahmsrssgpl sygfqsleas inaedvqddi
      241 ppnaanlrte mldnsseaai etsdksqltd kpakanktta isrhespete appllvtpts
      301 ptveklattp sgrsrtereq rsgntlyqel sfqdadalge mdegaditgs wvhpgeyass
      361 gmgkevenli mennellatk nalnivkddl ivkvdeltse qeilreevrg lqqarerlkq
      421 rvaaleeelk kvkeeaeaaa kaaksddeed vplaqrkrft rvemarvlme rnqykerfme
      481 lqeavrwtem irasktdpss isggkgsvwk ffsslftgpa drgtlvrgah tlphirysap
      541 tnqvvpassl damrrrtlkg rheifdqgdt iaseklvarr anerreqyrq vrahvrkedg
      601 rlqaygwslp gkpsapvrqp vpvpvycrpl qesepgmkiw cgagvnlsgg ktrdggcmig
      661 gsvfyaaeaq evssntkvea edavehldke iqenenqrve aeqleqqlss lvwictstqk
      721 mskvtvidan npagvlevfn vcqghllcia svpgakesdy iqgvneepvq ttngvndndg
      781 heenanveqi nqknkqetqa psdksknesd svseeqnnes tesdntnqni ttepqslesv
      841 dsetmnlgkv yfvkvnlesp psrlnekddt neenqndeve egtpiekmss iqptmwlgaq
      901 ngavfvhsav akwsvclhsv klkdaalaiv hvqgrvlval adgtvalfrr gadgqwdlsq
      961 yhvitlgspq hsircmaavs grtvwcgyrn kihvidpvlm tvectvdahp rresqvrqla
     1021 wlgegvwvsi rldstlrlyh ahtyqhlqdv diepyvskml gtgklgfsfv ritallissn
     1081 rlwigtgngv iisvplaedv yvvgaggsma vsrvqaasgk teapgvgvri fasdrsvtrg
     1141 sfipycsmah aqlsfhghrd avkmfvavpg hggqsavsdg sqpamlvlsg gegyidfrvg
     1201 dgedteesme rsnsavaana evhgeqshli vwqvqcplpv pmng
//
LOCUS       XP_034196094            1166 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196094
VERSION     XP_034196094.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340203.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1166
                     /product="death-associated protein kinase 1 isoform X1"
                     /calculated_mol_wt=130006
     Region          53..313
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(65..68,71,73,86,88,120,136..139,178,182..183,
                     198..199)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <326..>440
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          326..353
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(328..329,332..334,336..337,341,344,353,355,357,
                     361..362,365..367,369..370,374,377,386,388,390,394..395,
                     398..400,402..403,407,410,419)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          355..386
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          388..419
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1068..1144
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1166
                     /gene="LOC117611833"
                     /coded_by="XM_034340203.2:130..3630"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mmpgtsrqlg tlngwqmfns flqfntiegc kacqqrfqyt spewdtlmev hhepieknyk
       61 lleeigkgqf aivrkcmeik tgelyaakim rkrrvargva aadiareagl larlrhpniv
      121 slhkvvdtgt tvvlllelis ggelfhwvps geleaahvvr qvlmalnhlh shqvahldik
      181 penillstpp pmpsiklidl glshrlipgs ehralfgtpe fvapeivnye plslgtdlwa
      241 vgvltyills gaspflgedk qetyanvaac qyqfdneyfs tvseiakdfi rsllikdpke
      301 rgtaesclkh pwilteseas qglgeamisa vkrgcieavs qlllqgapln vkdskedtll
      361 hlacetgdeg mvtfliesgi dldtpnkkgl tplhvaarhg finlvrhlcl agcdvdktnr
      421 giradvtaik yghpdiasll dklrnpiqre nyikqlqpth rpidrlhirl lghcasgkss
      481 linslksgif sfgffrrsrs qnytakreps ieldvtskhg slsfeysdne yegtqgvdws
      541 rgnvggecif welcgredfl asyhyvltfq qpavhlitvs lrepltvqlq qvkfwlrfit
      601 driaptnfgf ggkyndvkvi lvgtyapepl apnsnsgsll apllpflkef tpvlgdepqm
      661 vavdatnpss pglkllrsyl nnarmeflee gpevaesilr rdtpraaasy vplanldkqs
      721 alvwtglaea wrsyvqslev plvmltqeef lrevrkinpl vclehcrhlg lqlqnlgecm
      781 llpgnlivls pewfwqdvin wqlspeqrgr lggrttgvya ledfqarcpc pagqalqalq
      841 avnlcvpcev dddeveyeip clnlverlpg lwepwkpcsn ilphtglrls pseaplyhli
      901 aifphlqsql rkitqswdps nsdlyqwwrg sklcigpces iitfededqs yvevrvrgpr
      961 gtsaqcfall siildavett ielvapgmll erhwlspsql reydeiihsw epapmisali
     1021 dkgleeatlk nplngkeetv weivgcglpl ienclpgpkq pikyiksavq rrlaqmldpp
     1081 drhgrdwcll avrlglgdrv aqldstvdsp tlrllnnaga dctvgslilq lraldredav
     1141 hlllsytpty ilstgtdset gsnlsr
//
LOCUS       XP_034196095            1142 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196095
VERSION     XP_034196095.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340204.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1142
                     /product="death-associated protein kinase 1 isoform X2"
                     /calculated_mol_wt=127287
     Region          29..289
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(41..44,47,49,62,64,96,112..115,154,158..159,
                     174..175)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <302..>416
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          302..329
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(304..305,308..310,312..313,317,320,329,331,333,
                     337..338,341..343,345..346,350,353,362,364,366,370..371,
                     374..376,378..379,383,386,395)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          331..362
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          364..395
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1044..1120
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1142
                     /gene="LOC117611833"
                     /coded_by="XM_034340204.2:216..3644"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mqsvpttfsi yeyclyspew dtlmevhhep ieknykllee igkgqfaivr kcmeiktgel
       61 yaakimrkrr vargvaaadi areagllarl rhpnivslhk vvdtgttvvl llelisggel
      121 fhwvpsgele aahvvrqvlm alnhlhshqv ahldikpeni llstpppmps iklidlglsh
      181 rlipgsehra lfgtpefvap eivnyeplsl gtdlwavgvl tyillsgasp flgedkqety
      241 anvaacqyqf dneyfstvse iakdfirsll ikdpkergta esclkhpwil teseasqglg
      301 eamisavkrg cieavsqlll qgaplnvkds kedtllhlac etgdegmvtf liesgidldt
      361 pnkkgltplh vaarhgfinl vrhlclagcd vdktnrgira dvtaikyghp diaslldklr
      421 npiqrenyik qlqpthrpid rlhirllghc asgksslins lksgifsfgf frrsrsqnyt
      481 akrepsield vtskhgslsf eysdneyegt qgvdwsrgnv ggecifwelc gredflasyh
      541 yvltfqqpav hlitvslrep ltvqlqqvkf wlrfitdria ptnfgfggky ndvkvilvgt
      601 yapeplapns nsgsllapll pflkeftpvl gdepqmvavd atnpsspglk llrsylnnar
      661 mefleegpev aesilrrdtp raaasyvpla nldkqsalvw tglaeawrsy vqslevplvm
      721 ltqeeflrev rkinplvcle hcrhlglqlq nlgecmllpg nlivlspewf wqdvinwqls
      781 peqrgrlggr ttgvyaledf qarcpcpagq alqalqavnl cvpcevddde veyeipclnl
      841 verlpglwep wkpcsnilph tglrlspsea plyhliaifp hlqsqlrkit qswdpsnsdl
      901 yqwwrgsklc igpcesiitf ededqsyvev rvrgprgtsa qcfallsiil davettielv
      961 apgmllerhw lspsqlreyd eiihswepap misalidkgl eeatlknpln gkeetvweiv
     1021 gcglplienc lpgpkqpiky iksavqrrla qmldppdrhg rdwcllavrl glgdrvaqld
     1081 stvdsptlrl lnnagadctv gslilqlral dredavhlll sytptyilst gtdsetgsnl
     1141 sr
//
LOCUS       XP_034196096            1139 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196096
VERSION     XP_034196096.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340205.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1139
                     /product="death-associated protein kinase 1 isoform X3"
                     /calculated_mol_wt=126717
     Region          26..286
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(38..41,44,46,59,61,93,109..112,151,155..156,
                     171..172)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <299..>413
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          299..326
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(301..302,305..307,309..310,314,317,326,328,330,
                     334..335,338..340,342..343,347,350,359,361,363,367..368,
                     371..373,375..376,380,383,392)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          328..359
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          361..392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1041..1117
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1139
                     /gene="LOC117611833"
                     /coded_by="XM_034340205.2:130..3549"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mmpgtsrqlg tlngpewdtl mevhhepiek nyklleeigk gqfaivrkcm eiktgelyaa
       61 kimrkrrvar gvaaadiare agllarlrhp nivslhkvvd tgttvvllle lisggelfhw
      121 vpsgeleaah vvrqvlmaln hlhshqvahl dikpenills tpppmpsikl idlglshrli
      181 pgsehralfg tpefvapeiv nyeplslgtd lwavgvltyi llsgaspflg edkqetyanv
      241 aacqyqfdne yfstvseiak dfirsllikd pkergtaesc lkhpwiltes easqglgeam
      301 isavkrgcie avsqlllqga plnvkdsked tllhlacetg degmvtflie sgidldtpnk
      361 kgltplhvaa rhgfinlvrh lclagcdvdk tnrgiradvt aikyghpdia slldklrnpi
      421 qrenyikqlq pthrpidrlh irllghcasg ksslinslks gifsfgffrr srsqnytakr
      481 epsieldvts khgslsfeys dneyegtqgv dwsrgnvgge cifwelcgre dflasyhyvl
      541 tfqqpavhli tvslrepltv qlqqvkfwlr fitdriaptn fgfggkyndv kvilvgtyap
      601 eplapnsnsg sllapllpfl keftpvlgde pqmvavdatn psspglkllr sylnnarmef
      661 leegpevaes ilrrdtpraa asyvplanld kqsalvwtgl aeawrsyvqs levplvmltq
      721 eeflrevrki nplvclehcr hlglqlqnlg ecmllpgnli vlspewfwqd vinwqlspeq
      781 rgrlggrttg vyaledfqar cpcpagqalq alqavnlcvp cevdddevey eipclnlver
      841 lpglwepwkp csnilphtgl rlspseaply hliaifphlq sqlrkitqsw dpsnsdlyqw
      901 wrgsklcigp cesiitfede dqsyvevrvr gprgtsaqcf allsiildav ettielvapg
      961 mllerhwlsp sqlreydeii hswepapmis alidkgleea tlknplngke etvweivgcg
     1021 lplienclpg pkqpikyiks avqrrlaqml dppdrhgrdw cllavrlglg drvaqldstv
     1081 dsptlrllnn agadctvgsl ilqlraldre davhlllsyt ptyilstgtd setgsnlsr
//
LOCUS       XP_034196097            1136 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196097
VERSION     XP_034196097.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340206.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1136
                     /product="death-associated protein kinase 1 isoform X4"
                     /calculated_mol_wt=126756
     Region          53..313
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(65..68,71,73,86,88,120,136..139,178,182..183,
                     198..199)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <326..>440
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          326..353
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(328..329,332..334,336..337,341,344,353,355,357,
                     361..362,365..367,369..370,374,377,386,388,390,394..395,
                     398..400,402..403,407,410,419)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          355..386
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          388..419
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1038..1114
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1136
                     /gene="LOC117611833"
                     /coded_by="XM_034340206.2:130..3540"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mmpgtsrqlg tlngwqmfns flqfntiegc kacqqrfqyt spewdtlmev hhepieknyk
       61 lleeigkgqf aivrkcmeik tgelyaakim rkrrvargva aadiareagl larlrhpniv
      121 slhkvvdtgt tvvlllelis ggelfhwvps geleaahvvr qvlmalnhlh shqvahldik
      181 penillstpp pmpsiklidl glshrlipgs ehralfgtpe fvapeivnye plslgtdlwa
      241 vgvltyills gaspflgedk qetyanvaac qyqfdneyfs tvseiakdfi rsllikdpke
      301 rgtaesclkh pwilteseas qglgeamisa vkrgcieavs qlllqgapln vkdskedtll
      361 hlacetgdeg mvtfliesgi dldtpnkkgl tplhvaarhg finlvrhlcl agcdvdktnr
      421 giradvtaik yghpdiasll dklrnpiqre nyikqlqpth rpidrlhirl lghcasgkss
      481 linslksgif sfgffrrsrs qnytakreps ieldvtskhg slsfeysdne yegtqgvdws
      541 rgnvggecif welcgredfl asyhyvltfq qpavhlitvs lrepltvqlq qvkfwlrfit
      601 driaptnfgf ggkyndvkvi lvgtyapepl apnsnsgsll apllpflkef tpvlgdepqm
      661 vavdatnpss pglkllrsyl nnarmefles alvwtglaea wrsyvqslev plvmltqeef
      721 lrevrkinpl vclehcrhlg lqlqnlgecm llpgnlivls pewfwqdvin wqlspeqrgr
      781 lggrttgvya ledfqarcpc pagqalqalq avnlcvpcev dddeveyeip clnlverlpg
      841 lwepwkpcsn ilphtglrls pseaplyhli aifphlqsql rkitqswdps nsdlyqwwrg
      901 sklcigpces iitfededqs yvevrvrgpr gtsaqcfall siildavett ielvapgmll
      961 erhwlspsql reydeiihsw epapmisali dkgleeatlk nplngkeetv weivgcglpl
     1021 ienclpgpkq pikyiksavq rrlaqmldpp drhgrdwcll avrlglgdrv aqldstvdsp
     1081 tlrllnnaga dctvgslilq lraldredav hlllsytpty ilstgtdset gsnlsr
//
LOCUS       XP_034196098            1119 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196098
VERSION     XP_034196098.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340207.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1119
                     /product="death-associated protein kinase 1 isoform X5"
                     /calculated_mol_wt=124531
     Region          6..266
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(18..21,24,26,39,41,73,89..92,131,135..136,151..152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <279..>393
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          279..306
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(281..282,285..287,289..290,294,297,306,308,310,
                     314..315,318..320,322..323,327,330,339,341,343,347..348,
                     351..353,355..356,360,363,372)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          308..339
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          341..372
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1021..1097
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1119
                     /gene="LOC117611833"
                     /coded_by="XM_034340207.2:356..3715"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mevhhepiek nyklleeigk gqfaivrkcm eiktgelyaa kimrkrrvar gvaaadiare
       61 agllarlrhp nivslhkvvd tgttvvllle lisggelfhw vpsgeleaah vvrqvlmaln
      121 hlhshqvahl dikpenills tpppmpsikl idlglshrli pgsehralfg tpefvapeiv
      181 nyeplslgtd lwavgvltyi llsgaspflg edkqetyanv aacqyqfdne yfstvseiak
      241 dfirsllikd pkergtaesc lkhpwiltes easqglgeam isavkrgcie avsqlllqga
      301 plnvkdsked tllhlacetg degmvtflie sgidldtpnk kgltplhvaa rhgfinlvrh
      361 lclagcdvdk tnrgiradvt aikyghpdia slldklrnpi qrenyikqlq pthrpidrlh
      421 irllghcasg ksslinslks gifsfgffrr srsqnytakr epsieldvts khgslsfeys
      481 dneyegtqgv dwsrgnvgge cifwelcgre dflasyhyvl tfqqpavhli tvslrepltv
      541 qlqqvkfwlr fitdriaptn fgfggkyndv kvilvgtyap eplapnsnsg sllapllpfl
      601 keftpvlgde pqmvavdatn psspglkllr sylnnarmef leegpevaes ilrrdtpraa
      661 asyvplanld kqsalvwtgl aeawrsyvqs levplvmltq eeflrevrki nplvclehcr
      721 hlglqlqnlg ecmllpgnli vlspewfwqd vinwqlspeq rgrlggrttg vyaledfqar
      781 cpcpagqalq alqavnlcvp cevdddevey eipclnlver lpglwepwkp csnilphtgl
      841 rlspseaply hliaifphlq sqlrkitqsw dpsnsdlyqw wrgsklcigp cesiitfede
      901 dqsyvevrvr gprgtsaqcf allsiildav ettielvapg mllerhwlsp sqlreydeii
      961 hswepapmis alidkgleea tlknplngke etvweivgcg lplienclpg pkqpikyiks
     1021 avqrrlaqml dppdrhgrdw cllavrlglg drvaqldstv dsptlrllnn agadctvgsl
     1081 ilqlraldre davhlllsyt ptyilstgtd setgsnlsr
//
LOCUS       XP_034196099            1119 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196099
VERSION     XP_034196099.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340208.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1119
                     /product="death-associated protein kinase 1 isoform X5"
                     /calculated_mol_wt=124531
     Region          6..266
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(18..21,24,26,39,41,73,89..92,131,135..136,151..152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <279..>393
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          279..306
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(281..282,285..287,289..290,294,297,306,308,310,
                     314..315,318..320,322..323,327,330,339,341,343,347..348,
                     351..353,355..356,360,363,372)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          308..339
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          341..372
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1021..1097
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1119
                     /gene="LOC117611833"
                     /coded_by="XM_034340208.2:369..3728"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mevhhepiek nyklleeigk gqfaivrkcm eiktgelyaa kimrkrrvar gvaaadiare
       61 agllarlrhp nivslhkvvd tgttvvllle lisggelfhw vpsgeleaah vvrqvlmaln
      121 hlhshqvahl dikpenills tpppmpsikl idlglshrli pgsehralfg tpefvapeiv
      181 nyeplslgtd lwavgvltyi llsgaspflg edkqetyanv aacqyqfdne yfstvseiak
      241 dfirsllikd pkergtaesc lkhpwiltes easqglgeam isavkrgcie avsqlllqga
      301 plnvkdsked tllhlacetg degmvtflie sgidldtpnk kgltplhvaa rhgfinlvrh
      361 lclagcdvdk tnrgiradvt aikyghpdia slldklrnpi qrenyikqlq pthrpidrlh
      421 irllghcasg ksslinslks gifsfgffrr srsqnytakr epsieldvts khgslsfeys
      481 dneyegtqgv dwsrgnvgge cifwelcgre dflasyhyvl tfqqpavhli tvslrepltv
      541 qlqqvkfwlr fitdriaptn fgfggkyndv kvilvgtyap eplapnsnsg sllapllpfl
      601 keftpvlgde pqmvavdatn psspglkllr sylnnarmef leegpevaes ilrrdtpraa
      661 asyvplanld kqsalvwtgl aeawrsyvqs levplvmltq eeflrevrki nplvclehcr
      721 hlglqlqnlg ecmllpgnli vlspewfwqd vinwqlspeq rgrlggrttg vyaledfqar
      781 cpcpagqalq alqavnlcvp cevdddevey eipclnlver lpglwepwkp csnilphtgl
      841 rlspseaply hliaifphlq sqlrkitqsw dpsnsdlyqw wrgsklcigp cesiitfede
      901 dqsyvevrvr gprgtsaqcf allsiildav ettielvapg mllerhwlsp sqlreydeii
      961 hswepapmis alidkgleea tlknplngke etvweivgcg lplienclpg pkqpikyiks
     1021 avqrrlaqml dppdrhgrdw cllavrlglg drvaqldstv dsptlrllnn agadctvgsl
     1081 ilqlraldre davhlllsyt ptyilstgtd setgsnlsr
//
LOCUS       XP_034196100            1119 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196100
VERSION     XP_034196100.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340209.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1119
                     /product="death-associated protein kinase 1 isoform X5"
                     /calculated_mol_wt=124531
     Region          6..266
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(18..21,24,26,39,41,73,89..92,131,135..136,151..152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <279..>393
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          279..306
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(281..282,285..287,289..290,294,297,306,308,310,
                     314..315,318..320,322..323,327,330,339,341,343,347..348,
                     351..353,355..356,360,363,372)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          308..339
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          341..372
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1021..1097
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1119
                     /gene="LOC117611833"
                     /coded_by="XM_034340209.2:81..3440"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mevhhepiek nyklleeigk gqfaivrkcm eiktgelyaa kimrkrrvar gvaaadiare
       61 agllarlrhp nivslhkvvd tgttvvllle lisggelfhw vpsgeleaah vvrqvlmaln
      121 hlhshqvahl dikpenills tpppmpsikl idlglshrli pgsehralfg tpefvapeiv
      181 nyeplslgtd lwavgvltyi llsgaspflg edkqetyanv aacqyqfdne yfstvseiak
      241 dfirsllikd pkergtaesc lkhpwiltes easqglgeam isavkrgcie avsqlllqga
      301 plnvkdsked tllhlacetg degmvtflie sgidldtpnk kgltplhvaa rhgfinlvrh
      361 lclagcdvdk tnrgiradvt aikyghpdia slldklrnpi qrenyikqlq pthrpidrlh
      421 irllghcasg ksslinslks gifsfgffrr srsqnytakr epsieldvts khgslsfeys
      481 dneyegtqgv dwsrgnvgge cifwelcgre dflasyhyvl tfqqpavhli tvslrepltv
      541 qlqqvkfwlr fitdriaptn fgfggkyndv kvilvgtyap eplapnsnsg sllapllpfl
      601 keftpvlgde pqmvavdatn psspglkllr sylnnarmef leegpevaes ilrrdtpraa
      661 asyvplanld kqsalvwtgl aeawrsyvqs levplvmltq eeflrevrki nplvclehcr
      721 hlglqlqnlg ecmllpgnli vlspewfwqd vinwqlspeq rgrlggrttg vyaledfqar
      781 cpcpagqalq alqavnlcvp cevdddevey eipclnlver lpglwepwkp csnilphtgl
      841 rlspseaply hliaifphlq sqlrkitqsw dpsnsdlyqw wrgsklcigp cesiitfede
      901 dqsyvevrvr gprgtsaqcf allsiildav ettielvapg mllerhwlsp sqlreydeii
      961 hswepapmis alidkgleea tlknplngke etvweivgcg lplienclpg pkqpikyiks
     1021 avqrrlaqml dppdrhgrdw cllavrlglg drvaqldstv dsptlrllnn agadctvgsl
     1081 ilqlraldre davhlllsyt ptyilstgtd setgsnlsr
//
LOCUS       XP_076546793            1119 aa            linear   INV 03-SEP-2025
DEFINITION  death-associated protein kinase 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546793
VERSION     XP_076546793.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690678.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1119
                     /product="death-associated protein kinase 1 isoform X5"
                     /calculated_mol_wt=124531
     Region          6..266
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(18..21,24,26,39,41,73,89..92,131,135..136,151..152)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Region          <279..>393
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          279..306
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(281..282,285..287,289..290,294,297,306,308,310,
                     314..315,318..320,322..323,327,330,339,341,343,347..348,
                     351..353,355..356,360,363,372)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          308..339
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          341..372
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1021..1097
                     /region_name="Death_DAPK1"
                     /note="Death domain found in death-associated protein
                     kinase 1; cd08782"
                     /db_xref="CDD:260052"
     CDS             1..1119
                     /gene="LOC117611833"
                     /coded_by="XM_076690678.1:42..3401"
                     /db_xref="GeneID:117611833"
ORIGIN      
        1 mevhhepiek nyklleeigk gqfaivrkcm eiktgelyaa kimrkrrvar gvaaadiare
       61 agllarlrhp nivslhkvvd tgttvvllle lisggelfhw vpsgeleaah vvrqvlmaln
      121 hlhshqvahl dikpenills tpppmpsikl idlglshrli pgsehralfg tpefvapeiv
      181 nyeplslgtd lwavgvltyi llsgaspflg edkqetyanv aacqyqfdne yfstvseiak
      241 dfirsllikd pkergtaesc lkhpwiltes easqglgeam isavkrgcie avsqlllqga
      301 plnvkdsked tllhlacetg degmvtflie sgidldtpnk kgltplhvaa rhgfinlvrh
      361 lclagcdvdk tnrgiradvt aikyghpdia slldklrnpi qrenyikqlq pthrpidrlh
      421 irllghcasg ksslinslks gifsfgffrr srsqnytakr epsieldvts khgslsfeys
      481 dneyegtqgv dwsrgnvgge cifwelcgre dflasyhyvl tfqqpavhli tvslrepltv
      541 qlqqvkfwlr fitdriaptn fgfggkyndv kvilvgtyap eplapnsnsg sllapllpfl
      601 keftpvlgde pqmvavdatn psspglkllr sylnnarmef leegpevaes ilrrdtpraa
      661 asyvplanld kqsalvwtgl aeawrsyvqs levplvmltq eeflrevrki nplvclehcr
      721 hlglqlqnlg ecmllpgnli vlspewfwqd vinwqlspeq rgrlggrttg vyaledfqar
      781 cpcpagqalq alqavnlcvp cevdddevey eipclnlver lpglwepwkp csnilphtgl
      841 rlspseaply hliaifphlq sqlrkitqsw dpsnsdlyqw wrgsklcigp cesiitfede
      901 dqsyvevrvr gprgtsaqcf allsiildav ettielvapg mllerhwlsp sqlreydeii
      961 hswepapmis alidkgleea tlknplngke etvweivgcg lplienclpg pkqpikyiks
     1021 avqrrlaqml dppdrhgrdw cllavrlglg drvaqldstv dsptlrllnn agadctvgsl
     1081 ilqlraldre davhlllsyt ptyilstgtd setgsnlsr
//
LOCUS       XP_034196102             604 aa            linear   INV 03-SEP-2025
DEFINITION  catalase [Osmia lignaria lignaria].
ACCESSION   XP_034196102
VERSION     XP_034196102.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340211.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196102.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..604
                     /product="catalase"
                     /calculated_mol_wt=67922
     Region          111..589
                     /region_name="KatE"
                     /note="Catalase [Inorganic ion transport and metabolism];
                     COG0753"
                     /db_xref="CDD:440516"
     CDS             1..604
                     /gene="Cat"
                     /coded_by="XM_034340211.2:16..1830"
                     /db_xref="GeneID:117611835"
ORIGIN      
        1 mpkrsscliq afkvvslgpp ltrnaaplrv fhstvsrhdk splllpaisc cserctqrsv
       61 ffdkkpiltl afqrislrny ccknskdndd dmsekqrdpa seqlneyksk aenkrpvllt
      121 gngvpiankt asltvgpngp illqdyvyld emshfdreri pervvhakga gafgyfevtn
      181 ditkyskasv fsqvgkrtpi avrfssvgge sgsadtvrdp rgfavkfyte dgiwdlvgnn
      241 tpiffvkdpi ffpsfihtqk rnpvthlkda dmfwdflslr pesthqvlfl fsdrgipdgh
      301 rhmngygsht fklvnannem vyckfhyktd qgiknipvdk aaelsasdpd ysirdlynai
      361 ashryptwsf siqvmtpsqa knfkwnpfdv tkvwphkefp lipvgklvld rnpenyfadv
      421 eqiafdpahm ipgiepspdk mlqgrlfayg dthrhrlgpn hlqlpvncpf kaisamnyqr
      481 dglmavhnqn gapnyfpnsf ngpkecpavr ppkfnvsgdv dryepqnedd fgqagmfyrd
      541 vlnadekkrl adnlvgslrn astfiverav knftqadadl grkltdglrk agvrinvfgk
      601 tasl
//
LOCUS       XP_034196103             284 aa            linear   INV 03-SEP-2025
DEFINITION  tropomyosin [Osmia lignaria lignaria].
ACCESSION   XP_034196103
VERSION     XP_034196103.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340212.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="tropomyosin"
                     /calculated_mol_wt=32855
     Region          48..282
                     /region_name="Tropomyosin"
                     /note="pfam00261"
                     /db_xref="CDD:459736"
     CDS             1..284
                     /gene="LOC117611836"
                     /coded_by="XM_034340212.2:212..1066"
                     /db_xref="GeneID:117611836"
ORIGIN      
        1 meaikkkiaa lkqemdaane kveanetkqk lenmradtln devrdlqkkl aqlerdyeva
       61 kanldrstad leqcekswsr aeqdrttltk rvqeieaslt kkeelrltaq tklgrasela
      121 ddarrmckvl edrsrldeer teklmqelkd arliaedada kseeiskklq lveeelegae
      181 ervktseaki ieredelfiv qnivkslevs eekanqrved fklqlkelkk klraaekrav
      241 maertvkkll kevdtkedel reekekykav cddmdatfae mtgy
//
LOCUS       XP_034196104             284 aa            linear   INV 03-SEP-2025
DEFINITION  tropomyosin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196104
VERSION     XP_034196104.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340213.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="tropomyosin isoform X1"
                     /calculated_mol_wt=33110
     Region          48..282
                     /region_name="Tropomyosin"
                     /note="pfam00261"
                     /db_xref="CDD:459736"
     CDS             1..284
                     /gene="LOC117611837"
                     /coded_by="XM_034340213.2:152..1006"
                     /db_xref="GeneID:117611837"
ORIGIN      
        1 mnaikkrlqt lkiekdlamd radvcdqqak eanrreeklr devrelakkl vqmerdlels
       61 kaqlkksnrd leqkervyiv tqselavlnr kmqqclqnle kseerrltaq aklaqameta
      121 edakrickvl enrskqdeer mdqlmaqlke arliaedadt ksdeisrkla fvedeleaae
      181 ervksseaki veredelfiv gnilkslevs eekanqrvee fkaqlkelkv klkaaekrai
      241 iaekmvkvfs keldaredfl frekekykyi cddmdstfae ltgy
//
LOCUS       XP_076546794             284 aa            linear   INV 03-SEP-2025
DEFINITION  tropomyosin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546794
VERSION     XP_076546794.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690679.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="tropomyosin isoform X1"
                     /calculated_mol_wt=33110
     Region          48..282
                     /region_name="Tropomyosin"
                     /note="pfam00261"
                     /db_xref="CDD:459736"
     CDS             1..284
                     /gene="LOC117611837"
                     /coded_by="XM_076690679.1:173..1027"
                     /db_xref="GeneID:117611837"
ORIGIN      
        1 mnaikkrlqt lkiekdlamd radvcdqqak eanrreeklr devrelakkl vqmerdlels
       61 kaqlkksnrd leqkervyiv tqselavlnr kmqqclqnle kseerrltaq aklaqameta
      121 edakrickvl enrskqdeer mdqlmaqlke arliaedadt ksdeisrkla fvedeleaae
      181 ervksseaki veredelfiv gnilkslevs eekanqrvee fkaqlkelkv klkaaekrai
      241 iaekmvkvfs keldaredfl frekekykyi cddmdstfae ltgy
//
LOCUS       XP_034196106             284 aa            linear   INV 03-SEP-2025
DEFINITION  tropomyosin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196106
VERSION     XP_034196106.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340215.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="tropomyosin isoform X1"
                     /calculated_mol_wt=33110
     Region          48..282
                     /region_name="Tropomyosin"
                     /note="pfam00261"
                     /db_xref="CDD:459736"
     CDS             1..284
                     /gene="LOC117611837"
                     /coded_by="XM_034340215.2:472..1326"
                     /db_xref="GeneID:117611837"
ORIGIN      
        1 mnaikkrlqt lkiekdlamd radvcdqqak eanrreeklr devrelakkl vqmerdlels
       61 kaqlkksnrd leqkervyiv tqselavlnr kmqqclqnle kseerrltaq aklaqameta
      121 edakrickvl enrskqdeer mdqlmaqlke arliaedadt ksdeisrkla fvedeleaae
      181 ervksseaki veredelfiv gnilkslevs eekanqrvee fkaqlkelkv klkaaekrai
      241 iaekmvkvfs keldaredfl frekekykyi cddmdstfae ltgy
//
LOCUS       XP_034196105             284 aa            linear   INV 03-SEP-2025
DEFINITION  tropomyosin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196105
VERSION     XP_034196105.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340214.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="tropomyosin isoform X1"
                     /calculated_mol_wt=33110
     Region          48..282
                     /region_name="Tropomyosin"
                     /note="pfam00261"
                     /db_xref="CDD:459736"
     CDS             1..284
                     /gene="LOC117611837"
                     /coded_by="XM_034340214.2:135..989"
                     /db_xref="GeneID:117611837"
ORIGIN      
        1 mnaikkrlqt lkiekdlamd radvcdqqak eanrreeklr devrelakkl vqmerdlels
       61 kaqlkksnrd leqkervyiv tqselavlnr kmqqclqnle kseerrltaq aklaqameta
      121 edakrickvl enrskqdeer mdqlmaqlke arliaedadt ksdeisrkla fvedeleaae
      181 ervksseaki veredelfiv gnilkslevs eekanqrvee fkaqlkelkv klkaaekrai
      241 iaekmvkvfs keldaredfl frekekykyi cddmdstfae ltgy
//
LOCUS       XP_034196107             284 aa            linear   INV 03-SEP-2025
DEFINITION  tropomyosin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196107
VERSION     XP_034196107.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340216.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="tropomyosin isoform X1"
                     /calculated_mol_wt=33110
     Region          48..282
                     /region_name="Tropomyosin"
                     /note="pfam00261"
                     /db_xref="CDD:459736"
     CDS             1..284
                     /gene="LOC117611837"
                     /coded_by="XM_034340216.2:461..1315"
                     /db_xref="GeneID:117611837"
ORIGIN      
        1 mnaikkrlqt lkiekdlamd radvcdqqak eanrreeklr devrelakkl vqmerdlels
       61 kaqlkksnrd leqkervyiv tqselavlnr kmqqclqnle kseerrltaq aklaqameta
      121 edakrickvl enrskqdeer mdqlmaqlke arliaedadt ksdeisrkla fvedeleaae
      181 ervksseaki veredelfiv gnilkslevs eekanqrvee fkaqlkelkv klkaaekrai
      241 iaekmvkvfs keldaredfl frekekykyi cddmdstfae ltgy
//
LOCUS       XP_076546795             233 aa            linear   INV 03-SEP-2025
DEFINITION  tropomyosin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546795
VERSION     XP_076546795.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690680.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="tropomyosin isoform X2"
                     /calculated_mol_wt=26864
     Region          48..>203
                     /region_name="Tropomyosin"
                     /note="pfam00261"
                     /db_xref="CDD:459736"
     CDS             1..233
                     /gene="LOC117611837"
                     /coded_by="XM_076690680.1:152..853"
                     /db_xref="GeneID:117611837"
ORIGIN      
        1 mnaikkrlqt lkiekdlamd radvcdqqak eanrreeklr devrelakkl vqmerdlels
       61 kaqlkksnrd leqkervyiv tqselavlnr kmqqclqnle kseerrltaq aklaqameta
      121 edakrickvl enrskqdeer mdqlmaqlke arliaedadt ksdeisrkla fvedeleaae
      181 ervksseaki veredelfiv gqseggriqs aiegiegeie scrkeghhrr kdg
//
LOCUS       XP_034196108             102 aa            linear   INV 03-SEP-2025
DEFINITION  defensin [Osmia lignaria lignaria].
ACCESSION   XP_034196108
VERSION     XP_034196108.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340217.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196108.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="defensin"
                     /calculated_mol_wt=11257
     Region          52..89
                     /region_name="DEFL_defensin-like"
                     /note="defensin-like domain found in bilateria defensins,
                     sapecins, tenecins, phormicins, and lucifensins; cd21806"
                     /db_xref="CDD:409639"
     CDS             1..102
                     /gene="LOC117611839"
                     /coded_by="XM_034340217.2:63..371"
                     /db_xref="GeneID:117611839"
ORIGIN      
        1 mkfnifltfl lvsvvalmaa ptsiiqdepk dempldairs eteradrqrr vtcdllsfkg
       61 iaehsacaan clsmgkaggr cengicicrk ttfkelwdkr fg
//
LOCUS       XP_034196109              94 aa            linear   INV 03-SEP-2025
DEFINITION  defensin-like [Osmia lignaria lignaria].
ACCESSION   XP_034196109
VERSION     XP_034196109.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340218.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..94
                     /product="defensin-like"
                     /calculated_mol_wt=10162
     Region          44..81
                     /region_name="DEFL_defensin-like"
                     /note="defensin-like domain found in bilateria defensins,
                     sapecins, tenecins, phormicins, and lucifensins; cd21806"
                     /db_xref="CDD:409639"
     CDS             1..94
                     /gene="LOC117611840"
                     /coded_by="XM_034340218.2:679..963"
                     /db_xref="GeneID:117611840"
ORIGIN      
        1 mvkiyflaal llvaiaaima qpvdneyepl vlpegrgdrh rrvtcdllty kgvvgdsaca
       61 anclsmgkag grchdgvctc rkttfkevwd krfg
//
LOCUS       XP_034196187            1758 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor at 64C [Osmia lignaria
            lignaria].
ACCESSION   XP_034196187
VERSION     XP_034196187.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340296.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196187.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1758
                     /product="rho guanine nucleotide exchange factor at 64C"
                     /calculated_mol_wt=198203
     Region          <582..787
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1363..1555
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1369,1373,1473,1502..1503,1506..1507,1509..1510,
                     1513..1514,1517..1518,1521,1551,1555)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     CDS             1..1758
                     /gene="RhoGEF64C"
                     /coded_by="XM_034340296.2:435..5711"
                     /db_xref="GeneID:117611878"
ORIGIN      
        1 mtmtsvavap gslgapssml tpkmyhlqqg lnstsasptp gknydtlskg kkswsvrlgl
       61 srqnqstddp gkgwgtisgg sgisrqmiyg dpwlygtvrs sraghepppr fmtppppppp
      121 gapvlvvcsc peflsgttrk fgncrkcggh rlagvplggt crlpsvssrs rpslagvlks
      181 siddpydqmr rnrlmeprtr arsisphrpl sqrdsrsqsv arntkerkss iegtcsrsew
      241 fdkeasyeet arksrasftg astgddwlee apsseqsrgq ststtpgrlv ripkkikgrd
      301 wtdrglvkst evksnqdevw kerpssagds rrsilecdvn pylllkkhkd eddlsddlsd
      361 naleqedsrp lsslfdsskv ksieripnrs avaaiggqri rvfnepreis ddeeippvtr
      421 ipipkvspkr pprrlkeakq tlksilkrgk vvetrrknvl fnvdnvifap ekpsevtrls
      481 wsrigricas greqrddees gdeeeddeee eeeeepislh dqrerynfit ipnirdpked
      541 rvrlkepris qdvcqnpvnv dgikvdqfvp sgnverafsk kkkenvqssr hnedtsrnfg
      601 dqsvhslrqe ntkeeveerk riteeeqgnk meedtevqik ieehedrfpr iavdekdlil
      661 ksevpenskr sslsrsqser ssnrpevsag dvlfmdtmri slsrklkdtp sslnsseqtl
      721 qredsiestk etienpnpnd stetehqqdv siakepeevp qdriqttikt elelltessn
      781 syernndhfr gmrptswspp pgkpkhryri sdpgvktkfs eqfesnaglk tswrtsscyn
      841 sskveigtpt tennvykitv sprasplvhr lqigpgtnes rrtsilingd ataptaettp
      901 dnkvtisvgg edsvynptvi svnsenlpri ktsgenrtlv ilenyksniv vesgkedpks
      961 klktrsesde edvsgknkea geksaersem kasnteksak fyvtndqksd qknpektkls
     1021 kealisklle dslrkareng eilnedsgea ilkilkqsll kskeyesses tleanysras
     1081 slnsegdfis tnlfleenpy evikepiyee ipdeppplpl sppptedyik driyfgdvdy
     1141 yrknnteqfl dtyltdnvfk ktnpediten ylskspedff kkmstspeee nisskfelln
     1201 flmdskdrai sveeeeddde edeedtegdl ealyeqkets lgdlsskssq isnvsdssee
     1261 cniiltssse tskaravdie rtdsgvgses sqasstrgvp rrwrtgnvss gpgsllsgip
     1321 qvisgdsklc edceqrldpl itdsgvvyap fvcrkcskkr verkeiitei vetelkygrd
     1381 lqiileefhr pmlragllts dqltaiflnv eellehnlvl aeklkdavef aqesgdedll
     1441 tvdvgkifle sermlhafes yctrqgsasl llqnlekeke llriflrvsq mentvlrrmn
     1501 lnsflmvpvq rvtkypllla rllkatpsvr pdiqeakekl kqaqanielh lehmnaeakd
     1561 vtstklwrri siiqngrrpi geqdmvnikl rkmavevlew aheeakfvle grllvaqptd
     1621 nnwrrgrtvk lapvtamlvt ngkpngedle fnddslfprq igikeatlll vkeklgrysl
     1681 lreplyldkc ivccetdled yfeiqelssk etfifkaedg artkrwcrtl qahaqslgaw
     1741 rkrrgalpni micgvarn
//
LOCUS       XP_034196188            1758 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor at 64C [Osmia lignaria
            lignaria].
ACCESSION   XP_034196188
VERSION     XP_034196188.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340297.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196188.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1758
                     /product="rho guanine nucleotide exchange factor at 64C"
                     /calculated_mol_wt=198203
     Region          <582..787
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1363..1555
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous
                     (DH) domain. It appears that PH domains invariably occur
                     C-terminal to RhoGEF/DH domains; cd00160"
                     /db_xref="CDD:238091"
     Site            order(1369,1373,1473,1502..1503,1506..1507,1509..1510,
                     1513..1514,1517..1518,1521,1551,1555)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     CDS             1..1758
                     /gene="RhoGEF64C"
                     /coded_by="XM_034340297.2:430..5706"
                     /db_xref="GeneID:117611878"
ORIGIN      
        1 mtmtsvavap gslgapssml tpkmyhlqqg lnstsasptp gknydtlskg kkswsvrlgl
       61 srqnqstddp gkgwgtisgg sgisrqmiyg dpwlygtvrs sraghepppr fmtppppppp
      121 gapvlvvcsc peflsgttrk fgncrkcggh rlagvplggt crlpsvssrs rpslagvlks
      181 siddpydqmr rnrlmeprtr arsisphrpl sqrdsrsqsv arntkerkss iegtcsrsew
      241 fdkeasyeet arksrasftg astgddwlee apsseqsrgq ststtpgrlv ripkkikgrd
      301 wtdrglvkst evksnqdevw kerpssagds rrsilecdvn pylllkkhkd eddlsddlsd
      361 naleqedsrp lsslfdsskv ksieripnrs avaaiggqri rvfnepreis ddeeippvtr
      421 ipipkvspkr pprrlkeakq tlksilkrgk vvetrrknvl fnvdnvifap ekpsevtrls
      481 wsrigricas greqrddees gdeeeddeee eeeeepislh dqrerynfit ipnirdpked
      541 rvrlkepris qdvcqnpvnv dgikvdqfvp sgnverafsk kkkenvqssr hnedtsrnfg
      601 dqsvhslrqe ntkeeveerk riteeeqgnk meedtevqik ieehedrfpr iavdekdlil
      661 ksevpenskr sslsrsqser ssnrpevsag dvlfmdtmri slsrklkdtp sslnsseqtl
      721 qredsiestk etienpnpnd stetehqqdv siakepeevp qdriqttikt elelltessn
      781 syernndhfr gmrptswspp pgkpkhryri sdpgvktkfs eqfesnaglk tswrtsscyn
      841 sskveigtpt tennvykitv sprasplvhr lqigpgtnes rrtsilingd ataptaettp
      901 dnkvtisvgg edsvynptvi svnsenlpri ktsgenrtlv ilenyksniv vesgkedpks
      961 klktrsesde edvsgknkea geksaersem kasnteksak fyvtndqksd qknpektkls
     1021 kealisklle dslrkareng eilnedsgea ilkilkqsll kskeyesses tleanysras
     1081 slnsegdfis tnlfleenpy evikepiyee ipdeppplpl sppptedyik driyfgdvdy
     1141 yrknnteqfl dtyltdnvfk ktnpediten ylskspedff kkmstspeee nisskfelln
     1201 flmdskdrai sveeeeddde edeedtegdl ealyeqkets lgdlsskssq isnvsdssee
     1261 cniiltssse tskaravdie rtdsgvgses sqasstrgvp rrwrtgnvss gpgsllsgip
     1321 qvisgdsklc edceqrldpl itdsgvvyap fvcrkcskkr verkeiitei vetelkygrd
     1381 lqiileefhr pmlragllts dqltaiflnv eellehnlvl aeklkdavef aqesgdedll
     1441 tvdvgkifle sermlhafes yctrqgsasl llqnlekeke llriflrvsq mentvlrrmn
     1501 lnsflmvpvq rvtkypllla rllkatpsvr pdiqeakekl kqaqanielh lehmnaeakd
     1561 vtstklwrri siiqngrrpi geqdmvnikl rkmavevlew aheeakfvle grllvaqptd
     1621 nnwrrgrtvk lapvtamlvt ngkpngedle fnddslfprq igikeatlll vkeklgrysl
     1681 lreplyldkc ivccetdled yfeiqelssk etfifkaedg artkrwcrtl qahaqslgaw
     1741 rkrrgalpni micgvarn
//
LOCUS       XP_034196189             550 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein C18orf63-like [Osmia lignaria lignaria].
ACCESSION   XP_034196189
VERSION     XP_034196189.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340298.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196189.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..550
                     /product="uncharacterized protein C18orf63-like"
                     /calculated_mol_wt=62759
     Region          15..288
                     /region_name="DUF4708"
                     /note="Domain of unknown function (DUF4708); pfam15813"
                     /db_xref="CDD:464889"
     CDS             1..550
                     /gene="LOC117611880"
                     /coded_by="XM_034340298.2:253..1905"
                     /db_xref="GeneID:117611880"
ORIGIN      
        1 mdtynvnekk ddtvlynttl palndlrcam ceidfieird ssaksnfhwk tmkcrllihl
       61 vsdliaspvs gtetfiivit hkqffdtgkl ekilrtvklv yrglqpvtie vykscliyti
      121 qakiaplwni adqyliqgkd fynftegara lkldvlmkdm kiclklhaek ikiscikled
      181 ylpsfvisqf ladpkgyidl svynlpfihv lpstkkgkll svskkmppkc vfknydqlrr
      241 hwknmygyal pktedgilyy eikflipkss ifiypnlcvm sgplniipnr dkgptiiqfl
      301 sdmlvklpav cnkklqiskd dpfntqlgna ssltdtllnn teleihssqn nstsnshfef
      361 stkyvdelkk dsssvptslk trlamihqlh nasfgestnt nlsikniren qsdmqpkkkf
      421 cvvnsasstt nrnfitskeg tisryfpiqk lhssnnites tqqekqdhvs lkeklpnvkp
      481 neqskislse tdenvnieam aknnqldqln snilsnwlrk hsiphnpngk kvelikkils
      541 hirntqqlri
//
LOCUS       XP_034196190             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611881 [Osmia lignaria lignaria].
ACCESSION   XP_034196190
VERSION     XP_034196190.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340299.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196190.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC117611881"
                     /calculated_mol_wt=54930
     Region          <24..178
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(43,45,49..50,53..55,57..58,62,65,77,79,81,85..86,
                     89..91,93..94,98,101,110,112,114,118..119,122..124,126,
                     129)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..77
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          79..110
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          429..483
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..486
                     /gene="LOC117611881"
                     /coded_by="XM_034340299.2:91..1551"
                     /db_xref="GeneID:117611881"
ORIGIN      
        1 msimscenni klqinanikq aveaeiikal ktedytlntv nsygenllhi saangclgvi
       61 teilqkwdsh dvidrknqfg wtplmlairn rdiktvkfll kqnvninest ylgmsvlgla
      121 aainkemfei vyeacpsalp ncindditpl ciaamkndkd lffrllelgf dvsksneytq
      181 immkqsivpe irnlgkrnls ienywndvsd nipieynien kcdikknnin nnnvlspnih
      241 tkpicvpnsl kddnnecnnn ividsnkylk pcalkliskq lecvphslis ptltctldei
      301 lpqspniyfm qnkqdeqinf nimtdienkk pienkdiita csgtndlsep hlsrlqsicp
      361 pnliiqteqe dldatlgyip efsplrspnv ppdindenvf gestptppry ktpprrmiln
      421 seeakmfvll kqyglsqhip ifleqevdvs lfltltdadl ieigienesd rkiilnviad
      481 crksmi
//
LOCUS       XP_076546796             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305777 [Osmia lignaria lignaria].
ACCESSION   XP_076546796
VERSION     XP_076546796.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690681.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC143305777"
                     /calculated_mol_wt=54930
     Region          <24..178
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(43,45,49..50,53..55,57..58,62,65,77,79,81,85..86,
                     89..91,93..94,98,101,110,112,114,118..119,122..124,126,
                     129)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..77
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          79..110
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          429..483
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..486
                     /gene="LOC143305777"
                     /coded_by="XM_076690681.1:91..1551"
                     /db_xref="GeneID:143305777"
ORIGIN      
        1 msimscenni klqinanikq aveaeiikal ktedytlntv nsygenllhi saangclgvi
       61 teilqkwdsh dvidrknqfg wtplmlairn rdiktvkfll kqnvninest ylgmsvlgla
      121 aainkemfei vyeacpsalp ncindditpl ciaamkndkd lffrllelgf dvsksneytq
      181 immkqsivpe irnlgkrnls ienywndvsd nipieynien kcdikknnin nnnvlspnih
      241 tkpicvpnsl kddnnecnnn ividsnkylk pcalkliskq lecvphslis ptltctldei
      301 lpqspniyfm qnkqdeqinf nimtdienkk pienkdiita csgtndlsep hlsrlqsicp
      361 pnliiqteqe dldatlgyip efsplrspnv ppdindenvf gestptppry ktpprrmiln
      421 seeakmfvll kqyglsqhip ifleqevdvs lfltltdadl ieigienesd rkiilnviad
      481 crksmi
//
LOCUS       XP_076546797             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305778 [Osmia lignaria lignaria].
ACCESSION   XP_076546797
VERSION     XP_076546797.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690682.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC143305778"
                     /calculated_mol_wt=54930
     Region          <24..178
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(43,45,49..50,53..55,57..58,62,65,77,79,81,85..86,
                     89..91,93..94,98,101,110,112,114,118..119,122..124,126,
                     129)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..77
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          79..110
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          429..483
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..486
                     /gene="LOC143305778"
                     /coded_by="XM_076690682.1:91..1551"
                     /db_xref="GeneID:143305778"
ORIGIN      
        1 msimscenni klqinanikq aveaeiikal ktedytlntv nsygenllhi saangclgvi
       61 teilqkwdsh dvidrknqfg wtplmlairn rdiktvkfll kqnvninest ylgmsvlgla
      121 aainkemfei vyeacpsalp ncindditpl ciaamkndkd lffrllelgf dvsksneytq
      181 immkqsivpe irnlgkrnls ienywndvsd nipieynien kcdikknnin nnnvlspnih
      241 tkpicvpnsl kddnnecnnn ividsnkylk pcalkliskq lecvphslis ptltctldei
      301 lpqspniyfm qnkqdeqinf nimtdienkk pienkdiita csgtndlsep hlsrlqsicp
      361 pnliiqteqe dldatlgyip efsplrspnv ppdindenvf gestptppry ktpprrmiln
      421 seeakmfvll kqyglsqhip ifleqevdvs lfltltdadl ieigienesd rkiilnviad
      481 crksmi
//
LOCUS       XP_076546798             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305779 [Osmia lignaria lignaria].
ACCESSION   XP_076546798
VERSION     XP_076546798.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690683.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC143305779"
                     /calculated_mol_wt=54930
     Region          <24..178
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(43,45,49..50,53..55,57..58,62,65,77,79,81,85..86,
                     89..91,93..94,98,101,110,112,114,118..119,122..124,126,
                     129)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..77
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          79..110
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          429..483
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..486
                     /gene="LOC143305779"
                     /coded_by="XM_076690683.1:91..1551"
                     /db_xref="GeneID:143305779"
ORIGIN      
        1 msimscenni klqinanikq aveaeiikal ktedytlntv nsygenllhi saangclgvi
       61 teilqkwdsh dvidrknqfg wtplmlairn rdiktvkfll kqnvninest ylgmsvlgla
      121 aainkemfei vyeacpsalp ncindditpl ciaamkndkd lffrllelgf dvsksneytq
      181 immkqsivpe irnlgkrnls ienywndvsd nipieynien kcdikknnin nnnvlspnih
      241 tkpicvpnsl kddnnecnnn ividsnkylk pcalkliskq lecvphslis ptltctldei
      301 lpqspniyfm qnkqdeqinf nimtdienkk pienkdiita csgtndlsep hlsrlqsicp
      361 pnliiqteqe dldatlgyip efsplrspnv ppdindenvf gestptppry ktpprrmiln
      421 seeakmfvll kqyglsqhip ifleqevdvs lfltltdadl ieigienesd rkiilnviad
      481 crksmi
//
LOCUS       XP_076546799             486 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305780 [Osmia lignaria lignaria].
ACCESSION   XP_076546799
VERSION     XP_076546799.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690684.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="uncharacterized protein LOC143305780"
                     /calculated_mol_wt=54930
     Region          <24..178
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(43,45,49..50,53..55,57..58,62,65,77,79,81,85..86,
                     89..91,93..94,98,101,110,112,114,118..119,122..124,126,
                     129)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          43..77
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          79..110
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          429..483
                     /region_name="SAM_superfamily"
                     /note="SAM (Sterile alpha motif); cl15755"
                     /db_xref="CDD:472832"
     CDS             1..486
                     /gene="LOC143305780"
                     /coded_by="XM_076690684.1:91..1551"
                     /db_xref="GeneID:143305780"
ORIGIN      
        1 msimscenni klqinanikq aveaeiikal ktedytlntv nsygenllhi saangclgvi
       61 teilqkwdsh dvidrknqfg wtplmlairn rdiktvkfll kqnvninest ylgmsvlgla
      121 aainkemfei vyeacpsalp ncindditpl ciaamkndkd lffrllelgf dvsksneytq
      181 immkqsivpe irnlgkrnls ienywndvsd nipieynien kcdikknnin nnnvlspnih
      241 tkpicvpnsl kddnnecnnn ividsnkylk pcalkliskq lecvphslis ptltctldei
      301 lpqspniyfm qnkqdeqinf nimtdienkk pienkdiita csgtndlsep hlsrlqsicp
      361 pnliiqteqe dldatlgyip efsplrspnv ppdindenvf gestptppry ktpprrmiln
      421 seeakmfvll kqyglsqhip ifleqevdvs lfltltdadl ieigienesd rkiilnviad
      481 crksmi
//
LOCUS       XP_034196191             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611882 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196191
VERSION     XP_034196191.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340300.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196191.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC117611882 isoform X1"
                     /calculated_mol_wt=54820
     CDS             1..479
                     /gene="LOC117611882"
                     /coded_by="XM_034340300.2:325..1764"
                     /db_xref="GeneID:117611882"
ORIGIN      
        1 mfclkgrgnn dgrfkesske edfgwwinne ddfksprsnq trecqslgss esmlscadsd
       61 dsesyflaqe frddynddce rieltslihh dldidliekd aipkeegita fsakeakqyf
      121 datetksnmf qaicytperl vrsqeyrilt psprylsicq srvtgetenh rntfrsqrrl
      181 nylidskqlg ttllssnsee malstitche wrsdqricnd nlstknnndp kmedkclngi
      241 dgknsnslqt snviskkssy erftvssedg ekmgihsssl ednssirsnd wsldshnsqs
      301 tplclsdela ttsykldtsq ercsavnevv silevldtnp ekatvlleed rfcdstsshd
      361 ritrlalaie pdtnvsgvve hpdnlvknlr nkverlqtns kdiykdisdl rrsfqrdeqs
      421 itnitsnask lrqdiqelrh lddllnllrg elkriskrnw pfiegrteey nseemnliv
//
LOCUS       XP_034196192             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611882 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196192
VERSION     XP_034196192.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340301.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196192.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC117611882 isoform X1"
                     /calculated_mol_wt=54820
     CDS             1..479
                     /gene="LOC117611882"
                     /coded_by="XM_034340301.2:361..1800"
                     /db_xref="GeneID:117611882"
ORIGIN      
        1 mfclkgrgnn dgrfkesske edfgwwinne ddfksprsnq trecqslgss esmlscadsd
       61 dsesyflaqe frddynddce rieltslihh dldidliekd aipkeegita fsakeakqyf
      121 datetksnmf qaicytperl vrsqeyrilt psprylsicq srvtgetenh rntfrsqrrl
      181 nylidskqlg ttllssnsee malstitche wrsdqricnd nlstknnndp kmedkclngi
      241 dgknsnslqt snviskkssy erftvssedg ekmgihsssl ednssirsnd wsldshnsqs
      301 tplclsdela ttsykldtsq ercsavnevv silevldtnp ekatvlleed rfcdstsshd
      361 ritrlalaie pdtnvsgvve hpdnlvknlr nkverlqtns kdiykdisdl rrsfqrdeqs
      421 itnitsnask lrqdiqelrh lddllnllrg elkriskrnw pfiegrteey nseemnliv
//
LOCUS       XP_034196193             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611882 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196193
VERSION     XP_034196193.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340302.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196193.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC117611882 isoform X1"
                     /calculated_mol_wt=54820
     CDS             1..479
                     /gene="LOC117611882"
                     /coded_by="XM_034340302.2:83..1522"
                     /db_xref="GeneID:117611882"
ORIGIN      
        1 mfclkgrgnn dgrfkesske edfgwwinne ddfksprsnq trecqslgss esmlscadsd
       61 dsesyflaqe frddynddce rieltslihh dldidliekd aipkeegita fsakeakqyf
      121 datetksnmf qaicytperl vrsqeyrilt psprylsicq srvtgetenh rntfrsqrrl
      181 nylidskqlg ttllssnsee malstitche wrsdqricnd nlstknnndp kmedkclngi
      241 dgknsnslqt snviskkssy erftvssedg ekmgihsssl ednssirsnd wsldshnsqs
      301 tplclsdela ttsykldtsq ercsavnevv silevldtnp ekatvlleed rfcdstsshd
      361 ritrlalaie pdtnvsgvve hpdnlvknlr nkverlqtns kdiykdisdl rrsfqrdeqs
      421 itnitsnask lrqdiqelrh lddllnllrg elkriskrnw pfiegrteey nseemnliv
//
LOCUS       XP_034196194             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611882 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196194
VERSION     XP_034196194.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340303.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196194.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC117611882 isoform X1"
                     /calculated_mol_wt=54820
     CDS             1..479
                     /gene="LOC117611882"
                     /coded_by="XM_034340303.2:82..1521"
                     /db_xref="GeneID:117611882"
ORIGIN      
        1 mfclkgrgnn dgrfkesske edfgwwinne ddfksprsnq trecqslgss esmlscadsd
       61 dsesyflaqe frddynddce rieltslihh dldidliekd aipkeegita fsakeakqyf
      121 datetksnmf qaicytperl vrsqeyrilt psprylsicq srvtgetenh rntfrsqrrl
      181 nylidskqlg ttllssnsee malstitche wrsdqricnd nlstknnndp kmedkclngi
      241 dgknsnslqt snviskkssy erftvssedg ekmgihsssl ednssirsnd wsldshnsqs
      301 tplclsdela ttsykldtsq ercsavnevv silevldtnp ekatvlleed rfcdstsshd
      361 ritrlalaie pdtnvsgvve hpdnlvknlr nkverlqtns kdiykdisdl rrsfqrdeqs
      421 itnitsnask lrqdiqelrh lddllnllrg elkriskrnw pfiegrteey nseemnliv
//
LOCUS       XP_034196195             454 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611882 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196195
VERSION     XP_034196195.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340304.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196195.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..454
                     /product="uncharacterized protein LOC117611882 isoform X2"
                     /calculated_mol_wt=52023
     CDS             1..454
                     /gene="LOC117611882"
                     /coded_by="XM_034340304.2:369..1733"
                     /db_xref="GeneID:117611882"
ORIGIN      
        1 mfclkgrgnn dgrfkesske edfgwwinne ddfksprsnq trecqslgss esmlscadsd
       61 dsesyflaqe frddynddce rieltslihh dldidliekd aipkeeaicy tperlvrsqe
      121 yriltpspry lsicqsrvtg etenhrntfr sqrrlnylid skqlgttlls snseemalst
      181 itchewrsdq ricndnlstk nnndpkmedk clngidgkns nslqtsnvis kkssyerftv
      241 ssedgekmgi hssslednss irsndwslds hnsqstplcl sdelattsyk ldtsqercsa
      301 vnevvsilev ldtnpekatv lleedrfcds tsshdritrl alaiepdtnv sgvvehpdnl
      361 vknlrnkver lqtnskdiyk disdlrrsfq rdeqsitnit snasklrqdi qelrhlddll
      421 nllrgelkri skrnwpfieg rteeynseem nliv
//
LOCUS       XP_076546800             419 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611884 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546800
VERSION     XP_076546800.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690685.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..419
                     /product="uncharacterized protein LOC117611884 isoform X1"
                     /calculated_mol_wt=44968
     Region          32..115
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <307..335
                     /region_name="GAGA"
                     /note="GAGA factor; pfam09237"
                     /db_xref="CDD:462719"
     CDS             1..419
                     /gene="LOC117611884"
                     /coded_by="XM_076690685.1:137..1396"
                     /db_xref="GeneID:117611884"
ORIGIN      
        1 mgssgqlyrl swgefsssla stvqllrdhr dladvtlavg grtfsvhkiv lcaaspllld
       61 llkstpcqhp vvmlagiaad dlksllefvy rgevsvepsq lpsllqaahs lcihgltppt
      121 iltengeevp isaiptanea lsketvspyf plkrkrkrrk sssssgkwpr tsnttdnenr
      181 svdgtdnrsa gydqkdednt ehgddtaand rlkysnhrgc hspraqeaqp vssensqagq
      241 hqdqmseset hlhtlmpmqp ysplqipqfp gplnmgfppg iplplvtqsa tvgtvasgpt
      301 gnmnlskirg asdcpgvcpl cgatlrqarn lrrhllssck yrfssnsvhg stadsmmiei
      361 kpevelpgyn eptvtygtds gsstseqiic kpsplpspts hgsnvvspqn nipsptiar
//
LOCUS       XP_034196199             419 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611884 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196199
VERSION     XP_034196199.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340308.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196199.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..419
                     /product="uncharacterized protein LOC117611884 isoform X1"
                     /calculated_mol_wt=44968
     Region          32..115
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <307..335
                     /region_name="GAGA"
                     /note="GAGA factor; pfam09237"
                     /db_xref="CDD:462719"
     CDS             1..419
                     /gene="LOC117611884"
                     /coded_by="XM_034340308.2:142..1401"
                     /db_xref="GeneID:117611884"
ORIGIN      
        1 mgssgqlyrl swgefsssla stvqllrdhr dladvtlavg grtfsvhkiv lcaaspllld
       61 llkstpcqhp vvmlagiaad dlksllefvy rgevsvepsq lpsllqaahs lcihgltppt
      121 iltengeevp isaiptanea lsketvspyf plkrkrkrrk sssssgkwpr tsnttdnenr
      181 svdgtdnrsa gydqkdednt ehgddtaand rlkysnhrgc hspraqeaqp vssensqagq
      241 hqdqmseset hlhtlmpmqp ysplqipqfp gplnmgfppg iplplvtqsa tvgtvasgpt
      301 gnmnlskirg asdcpgvcpl cgatlrqarn lrrhllssck yrfssnsvhg stadsmmiei
      361 kpevelpgyn eptvtygtds gsstseqiic kpsplpspts hgsnvvspqn nipsptiar
//
LOCUS       XP_034196200             418 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611884 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196200
VERSION     XP_034196200.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340309.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196200.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..418
                     /product="uncharacterized protein LOC117611884 isoform X2"
                     /calculated_mol_wt=44897
     Region          32..115
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <306..334
                     /region_name="GAGA"
                     /note="GAGA factor; pfam09237"
                     /db_xref="CDD:462719"
     CDS             1..418
                     /gene="LOC117611884"
                     /coded_by="XM_034340309.2:137..1393"
                     /db_xref="GeneID:117611884"
ORIGIN      
        1 mgssgqlyrl swgefsssla stvqllrdhr dladvtlavg grtfsvhkiv lcaaspllld
       61 llkstpcqhp vvmlagiaad dlksllefvy rgevsvepsq lpsllqaahs lcihgltppt
      121 iltengeevp isaiptanea lsketvspyf plkrkrkrrk sssssgkwpr tsnttdnenr
      181 svdgtdnrsa gydqkdednt ehgddtandr lkysnhrgch spraqeaqpv ssensqagqh
      241 qdqmseseth lhtlmpmqpy splqipqfpg plnmgfppgi plplvtqsat vgtvasgptg
      301 nmnlskirga sdcpgvcplc gatlrqarnl rrhllsscky rfssnsvhgs tadsmmieik
      361 pevelpgyne ptvtygtdsg sstseqiick psplpsptsh gsnvvspqnn ipsptiar
//
LOCUS       XP_076546801             363 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611637 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546801
VERSION     XP_076546801.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690686.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..363
                     /product="uncharacterized protein LOC117611637 isoform X1"
                     /calculated_mol_wt=39930
     Region          <74..163
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Region          174..202
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(174,183,194..195)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(187,190,194,200..201)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            197..201
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..363
                     /gene="LOC117611637"
                     /coded_by="XM_076690686.1:526..1617"
                     /db_xref="GeneID:117611637"
ORIGIN      
        1 msvmsrgspw cwtapglrrs mffltiiilf gllspgvfge kskkyqisdv ckknfmrdly
       61 rkidgavlis qhekdldcti tfqthsilqr fmlrfdqlql dcndhlyiyd gahavssyka
      121 dlscrntkqs vgaiytrtnf vtlkyvtdsw gtitngfrlv itavkdpkha ckdfrctqne
      181 fcietdllcd gvnhcgdgsd eatsicanse tstilgmqki wfavalvfli lsvaglvtaa
      241 vlcfcrqrat tprhphnahn aqshppvsfp yvrierserr pasghdhveg hhghagtpge
      301 lrlgprtggn gchdppaagk laapcgpqar aqrgpgppll pgkvrshgsd sdisssqkde
      361 wfv
//
LOCUS       XP_076546802             350 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611637 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546802
VERSION     XP_076546802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690687.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="uncharacterized protein LOC117611637 isoform X2"
                     /calculated_mol_wt=38264
     Region          <61..150
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Region          161..189
                     /region_name="LDLa"
                     /note="Low Density Lipoprotein Receptor Class A domain, a
                     cysteine-rich repeat that plays a central role in
                     mammalian cholesterol metabolism; the receptor protein
                     binds LDL and transports it into cells by endocytosis; 7
                     successive cysteine-rich repeats of about...; cd00112"
                     /db_xref="CDD:238060"
     Site            order(161,170,181..182)
                     /site_type="active"
                     /note="putative binding surface [active]"
                     /db_xref="CDD:238060"
     Site            order(174,177,181,187..188)
                     /site_type="other"
                     /note="calcium-binding site [ion binding]"
                     /db_xref="CDD:238060"
     Site            184..188
                     /site_type="other"
                     /note="D-X-S-D-E motif"
                     /db_xref="CDD:238060"
     CDS             1..350
                     /gene="LOC117611637"
                     /coded_by="XM_076690687.1:133..1185"
                     /db_xref="GeneID:117611637"
ORIGIN      
        1 mcftegscaf llllaltlcq gpghaqaknd nyqisdvckk nfmrdlyrki dgavlisqhe
       61 kdldctitfq thsilqrfml rfdqlqldcn dhlyiydgah avssykadls crntkqsvga
      121 iytrtnfvtl kyvtdswgti tngfrlvita vkdpkhackd frctqnefci etdllcdgvn
      181 hcgdgsdeat sicansetst ilgmqkiwfa valvflilsv aglvtaavlc fcrqrattpr
      241 hphnahnaqs hppvsfpyvr ierserrpas ghdhveghhg hagtpgelrl gprtggngch
      301 dppaagklaa pcgpqaraqr gpgppllpgk vrshgsdsdi sssqkdewfv
//
LOCUS       XP_076546803             349 aa            linear   INV 03-SEP-2025
DEFINITION  lipase member H [Osmia lignaria lignaria].
ACCESSION   XP_076546803
VERSION     XP_076546803.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690688.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..349
                     /product="lipase member H"
                     /calculated_mol_wt=39168
     Region          63..326
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            178..182
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(180,205,271)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     CDS             1..349
                     /gene="LOC143305781"
                     /coded_by="XM_076690688.1:234..1283"
                     /db_xref="GeneID:143305781"
ORIGIN      
        1 maapvvwnal yvalslvlts rssmakqtet fvvsntidqg aggkdyfigp clvntnqtcp
       61 dkeitfflyt rrnfnesqqi lvdetssnla qtnfdpskpt kivihgydsn mmlsylvdvr
      121 meylktydyn viavdwhrla napcypivvq nvphvgdcla qlvqrlrdtg vqdihvigfs
      181 lgahvpgfaa ntlhpykisr itgldpampl fitddksrkl dagdaefvdv fhtnafiqgk
      241 iemsghidfy mngginqpgc wdrwnpfecd hhrsvmyfae sinsevgfwg wqcggfvnyl
      301 lglcpprypa tlagdkvdkk rrgfylvrth ahspyamgnf svnqldmyt
//
LOCUS       XP_034196197             320 aa            linear   INV 03-SEP-2025
DEFINITION  polyubiquitin-B [Osmia lignaria lignaria].
ACCESSION   XP_034196197
VERSION     XP_034196197.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340306.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196197.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="polyubiquitin-B"
                     /calculated_mol_wt=35686
     Region          1..76
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(6..9,11,34..37,40,42,44,46..49,68..76)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(8..12,31..35,40,42,44,46..49,68,70..76)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     Region          77..152
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(82..85,87,110..113,116,118,120,122..125,144..152)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(84..88,107..111,116,118,120,122..125,144,146..152)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     Region          153..228
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(158..161,163,186..189,192,194,196,198..201,220..228)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(160..164,183..187,192,194,196,198..201,220,222..228)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     Region          229..304
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(234..237,239,262..265,268,270,272,274..277,296..304)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(236..240,259..263,268,270,272,274..277,296,298..304)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     CDS             1..320
                     /gene="LOC117611883"
                     /coded_by="XM_034340306.2:165..1127"
                     /db_xref="GeneID:117611883"
ORIGIN      
        1 mqifvktltg ktitleveas dtienvkaki qdkegippdq qrlifagkql edgrtlsdyn
       61 iqkestlhlv lrlrggmqif vktltgktit leveasdtie nvkakiqdke gippdqqrli
      121 fagkqledgr tlsdyniqke stlhlvlrlr ggmqifvktl tgktitleve asdtienvka
      181 kiqdkegipp dqqrlifagk qledgrtlsd yniqkestlh lvlrlrggmq ifvktltgkt
      241 itleveasdt ienvkakiqd kegippdqqr lifagkqled grtlsdyniq kestlhlvlr
      301 lrggaethip vmaadelsys
//
LOCUS       XP_034196198             320 aa            linear   INV 03-SEP-2025
DEFINITION  polyubiquitin-B [Osmia lignaria lignaria].
ACCESSION   XP_034196198
VERSION     XP_034196198.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340307.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196198.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="polyubiquitin-B"
                     /calculated_mol_wt=35686
     Region          1..76
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(6..9,11,34..37,40,42,44,46..49,68..76)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(8..12,31..35,40,42,44,46..49,68,70..76)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     Region          77..152
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(82..85,87,110..113,116,118,120,122..125,144..152)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(84..88,107..111,116,118,120,122..125,144,146..152)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     Region          153..228
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(158..161,163,186..189,192,194,196,198..201,220..228)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(160..164,183..187,192,194,196,198..201,220,222..228)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     Region          229..304
                     /region_name="Ubl_ubiquitin"
                     /note="ubiquitin-like (Ubl) domain found in ubiquitin;
                     cd01803"
                     /db_xref="CDD:340501"
     Site            order(234..237,239,262..265,268,270,272,274..277,296..304)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:340501"
     Site            order(236..240,259..263,268,270,272,274..277,296,298..304)
                     /site_type="other"
                     /note="E1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340501"
     CDS             1..320
                     /gene="LOC117611883"
                     /coded_by="XM_034340307.2:141..1103"
                     /db_xref="GeneID:117611883"
ORIGIN      
        1 mqifvktltg ktitleveas dtienvkaki qdkegippdq qrlifagkql edgrtlsdyn
       61 iqkestlhlv lrlrggmqif vktltgktit leveasdtie nvkakiqdke gippdqqrli
      121 fagkqledgr tlsdyniqke stlhlvlrlr ggmqifvktl tgktitleve asdtienvka
      181 kiqdkegipp dqqrlifagk qledgrtlsd yniqkestlh lvlrlrggmq ifvktltgkt
      241 itleveasdt ienvkakiqd kegippdqqr lifagkqled grtlsdyniq kestlhlvlr
      301 lrggaethip vmaadelsys
//
LOCUS       XP_076546804             212 aa            linear   INV 03-SEP-2025
DEFINITION  longitudinals lacking protein-like [Osmia lignaria lignaria].
ACCESSION   XP_076546804
VERSION     XP_076546804.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690689.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..212
                     /product="longitudinals lacking protein-like"
                     /calculated_mol_wt=23012
     Region          32..115
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..212
                     /gene="LOC143305782"
                     /coded_by="XM_076690689.1:137..775"
                     /db_xref="GeneID:143305782"
ORIGIN      
        1 mgssgqlyrl swgefsssla stvqllrdhr dladvtlavg grtfsvhkiv lcaaspllld
       61 llkstpcqhp vvmlagiaad dlksllefvy rgevsvepsq lpsllqaahs lcihgltppt
      121 iltengeevp isaiptanea lsketvspyf plkrkrkrrk sssssgkwpr tsnttdnenr
      181 svdgtdnrsa gydqkdednt ehgddtgkky yi
//
LOCUS       XP_076546805             212 aa            linear   INV 03-SEP-2025
DEFINITION  longitudinals lacking protein-like [Osmia lignaria lignaria].
ACCESSION   XP_076546805
VERSION     XP_076546805.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690690.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..212
                     /product="longitudinals lacking protein-like"
                     /calculated_mol_wt=23012
     Region          32..115
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     CDS             1..212
                     /gene="LOC143305782"
                     /coded_by="XM_076690690.1:142..780"
                     /db_xref="GeneID:143305782"
ORIGIN      
        1 mgssgqlyrl swgefsssla stvqllrdhr dladvtlavg grtfsvhkiv lcaaspllld
       61 llkstpcqhp vvmlagiaad dlksllefvy rgevsvepsq lpsllqaahs lcihgltppt
      121 iltengeevp isaiptanea lsketvspyf plkrkrkrrk sssssgkwpr tsnttdnenr
      181 svdgtdnrsa gydqkdednt ehgddtgkky yi
//
LOCUS       XP_076546806             140 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305783 [Osmia lignaria lignaria].
ACCESSION   XP_076546806
VERSION     XP_076546806.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690691.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="uncharacterized protein LOC143305783"
                     /calculated_mol_wt=15279
     Region          <1..43
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..140
                     /gene="LOC143305783"
                     /coded_by="XM_076690691.1:123..545"
                     /db_xref="GeneID:143305783"
ORIGIN      
        1 mlagiaaddl ksllefvyrg evsvepsqlp sllqaahslc ihgltpptil tengeevpis
       61 aiptaneals ketvspyfpl krkrkrrkss sssgkwprts nttdnenrsv dgtdnrsagy
      121 dqkdednteh gddtgkkyyi
//
LOCUS       XP_076546807             140 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305784 [Osmia lignaria lignaria].
ACCESSION   XP_076546807
VERSION     XP_076546807.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690692.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="uncharacterized protein LOC143305784"
                     /calculated_mol_wt=15279
     Region          <1..43
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..140
                     /gene="LOC143305784"
                     /coded_by="XM_076690692.1:123..545"
                     /db_xref="GeneID:143305784"
ORIGIN      
        1 mlagiaaddl ksllefvyrg evsvepsqlp sllqaahslc ihgltpptil tengeevpis
       61 aiptaneals ketvspyfpl krkrkrrkss sssgkwprts nttdnenrsv dgtdnrsagy
      121 dqkdednteh gddtgkkyyi
//
LOCUS       XP_076546808             140 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143305785 [Osmia lignaria lignaria].
ACCESSION   XP_076546808
VERSION     XP_076546808.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690693.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..140
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..140
                     /product="uncharacterized protein LOC143305785"
                     /calculated_mol_wt=15339
     Region          <1..43
                     /region_name="BTB_POZ"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain superfamily; cl38908"
                     /db_xref="CDD:453885"
     CDS             1..140
                     /gene="LOC143305785"
                     /coded_by="XM_076690693.1:123..545"
                     /db_xref="GeneID:143305785"
ORIGIN      
        1 mlagiaaddl ksllefvyrg evsvepsqlp sllqaahflc ihgltpptil tengeevpis
       61 aiptaneals ketvspyfpl krkrkrrkss sssgkwprts nttdnenrsv dgtdnrsagy
      121 dqkdednteh gddtgkkyyi
//
LOCUS       XP_034196204             902 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611888 [Osmia lignaria lignaria].
ACCESSION   XP_034196204
VERSION     XP_034196204.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340313.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196204.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..902
                     /product="uncharacterized protein LOC117611888"
                     /calculated_mol_wt=104482
     Region          475..>518
                     /region_name="CAF1A"
                     /note="Chromatin assembly factor 1 subunit A; pfam12253"
                     /db_xref="CDD:463507"
     Region          602..>772
                     /region_name="CAF1-p150_C2"
                     /note="CAF1 complex subunit p150, region binding to
                     CAF1-p60 at C-term; pfam15539"
                     /db_xref="CDD:434777"
     CDS             1..902
                     /gene="LOC117611888"
                     /coded_by="XM_034340313.2:316..3024"
                     /db_xref="GeneID:117611888"
ORIGIN      
        1 mkmtdsnekd dcmieavtpi rtkkmkqaql pfqirsptqs pnvmktkkrk lsspsvgcks
       61 pkavkvskpa snkddakvet lrmdsndiei vesieiesvk entsnkvnav qksttpkqls
      121 tkqkkiekqd ksksncltkf lkktdtkedd anddqenpll eekdesvnes fdtetdanvs
      181 qkaeelvgav ndscdvnsdv vnlssdsndr ggdhlksnte qsmnsdsskt pkidkikktk
      241 ltptqqekkl lsikkkeemq krrrereikl eedrnnrrke kemkrkkred kikaekeqkl
      301 ferkmrkqkk eqeieqklke kqareedrkk keeekrkkee ekleaerkkq kaasnftsff
      361 vtkkleksfq eestlevknf mpfevkadmk mapisrrtln kkekllldek cnegnmqisd
      421 lylseikdkk iisrkstktw pleakedavt ilddendgts nivnqnivfe khrakllqfs
      481 enqrppywgt wrkrsrniks rkpfsrdtkw fnyevdsdee weeeepgesl hgseeekdee
      541 npedneydvd nefmvphgyl sneevradee deedmnpqtq kfklkvlgqq fesersrkts
      601 klkpkiigci wrgpnnefpp nvpertrefl taheawvrqi pvalpviien etmnptecgt
      661 psqqsalsss kksqvpeavl pdlirllhgn vhgkkflvke flaylnkege seyqmskarl
      721 mkkiceigkw mmcseegpmh lkacwyvsee vrkeymkedl plpnqwsytl tpkrrsifne
      781 ateklekedk dkekknvsli tqftkkisqe emkkhlnasp iettaqnsta ssslsvkpvg
      841 gkapkratfi tlskegepvs nvskeslsrt vntetkkrks deviildsse ddnvkvekkk
      901 kk
//
LOCUS       XP_034196205             614 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase Hip14 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196205
VERSION     XP_034196205.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340314.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..614
                     /product="palmitoyltransferase Hip14 isoform X1"
                     /calculated_mol_wt=68898
     Region          <44..247
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          72..98
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(75..76,79..81,83..84,88,91,100,102,109..110,
                     113..115,117..118,122,125,134,136,138,142..143,146..148,
                     150..151,155,158,167)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          100..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          136..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          209..240
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          413..549
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..614
                     /gene="Hip14"
                     /coded_by="XM_034340314.2:489..2333"
                     /db_xref="GeneID:117611889"
ORIGIN      
        1 myalkmqtac qseasgeped psshhqepvk ppvqdcssfd ivratqygal drvtelveag
       61 advnqpdset vtllhwaain nrkdivkyli akgavvdaig gelastplhw atrqghlptv
      121 vilmragadp tlrdsegfsc ihlaaqfght sivaylvakg vnpnmpdrsa mtplmwsayk
      181 vnsflfysld ptrllltlga shsltdnlhg ntalhwaiia enstaistlv hhgasldvpn
      241 irdetpmtll grhigaawlg hkisqeirer qgrtrtwcrd krirwycmvs tpfivfylig
      301 milqsgldyl vklgvfvtly ialylanhfv fderlfhiip msiylatkmw iyvtwifwlg
      361 ihaawylwll laggsvplwi cflqswrgdp gvitathedk lntiielaes ggfelhsfcs
      421 sclirrpmrs khcstcdrcv arfdhhcpwv nncigvhnhk yflgflasll glcivvlsas
      481 vqywqfecwt nltnghtadn ylvaaatcda wvmwvtants lhffwvgtll acqcyqimvl
      541 gmttnermna gryahfkqgn pfhrgaiqna adfcnisfcg ikakpssdwl hsfdhkqsie
      601 klpllatkdn fqyi
//
LOCUS       XP_034196206             614 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase Hip14 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196206
VERSION     XP_034196206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340315.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..614
                     /product="palmitoyltransferase Hip14 isoform X1"
                     /calculated_mol_wt=68898
     Region          <44..247
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          72..98
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(75..76,79..81,83..84,88,91,100,102,109..110,
                     113..115,117..118,122,125,134,136,138,142..143,146..148,
                     150..151,155,158,167)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          100..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          136..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          209..240
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          413..549
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..614
                     /gene="Hip14"
                     /coded_by="XM_034340315.2:535..2379"
                     /db_xref="GeneID:117611889"
ORIGIN      
        1 myalkmqtac qseasgeped psshhqepvk ppvqdcssfd ivratqygal drvtelveag
       61 advnqpdset vtllhwaain nrkdivkyli akgavvdaig gelastplhw atrqghlptv
      121 vilmragadp tlrdsegfsc ihlaaqfght sivaylvakg vnpnmpdrsa mtplmwsayk
      181 vnsflfysld ptrllltlga shsltdnlhg ntalhwaiia enstaistlv hhgasldvpn
      241 irdetpmtll grhigaawlg hkisqeirer qgrtrtwcrd krirwycmvs tpfivfylig
      301 milqsgldyl vklgvfvtly ialylanhfv fderlfhiip msiylatkmw iyvtwifwlg
      361 ihaawylwll laggsvplwi cflqswrgdp gvitathedk lntiielaes ggfelhsfcs
      421 sclirrpmrs khcstcdrcv arfdhhcpwv nncigvhnhk yflgflasll glcivvlsas
      481 vqywqfecwt nltnghtadn ylvaaatcda wvmwvtants lhffwvgtll acqcyqimvl
      541 gmttnermna gryahfkqgn pfhrgaiqna adfcnisfcg ikakpssdwl hsfdhkqsie
      601 klpllatkdn fqyi
//
LOCUS       XP_034196207             609 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase Hip14 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196207
VERSION     XP_034196207.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340316.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..609
                     /product="palmitoyltransferase Hip14 isoform X2"
                     /calculated_mol_wt=68240
     Region          40..252
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          72..98
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(75..76,79..81,83..84,88,91,100,102,109..110,
                     113..115,117..118,122,125,134,136,138,142..143,146..148,
                     150..151,155,158,167)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          100..134
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          136..167
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          169..201
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          204..235
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          408..544
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..609
                     /gene="Hip14"
                     /coded_by="XM_034340316.2:488..2317"
                     /db_xref="GeneID:117611889"
ORIGIN      
        1 myalkmqtac qseasgeped psshhqepvk ppvqdcssfd ivratqygal drvtelveag
       61 advnqpdset vtllhwaain nrkdivkyli akgavvdaig gelastplhw atrqghlptv
      121 vilmragadp tlrdsegfsc ihlaaqfght sivaylvakg vnpnmpdrsa mtplmwsayk
      181 vnsldptrll ltlgashslt dnlhgntalh waiiaensta istlvhhgas ldvpnirdet
      241 pmtllgrhig aawlghkisq eirerqgrtr twcrdkrirw ycmvstpfiv fyligmilqs
      301 gldylvklgv fvtlyialyl anhfvfderl fhiipmsiyl atkmwiyvtw ifwlgihaaw
      361 ylwlllaggs vplwicflqs wrgdpgvita thedklntii elaesggfel hsfcssclir
      421 rpmrskhcst cdrcvarfdh hcpwvnncig vhnhkyflgf lasllglciv vlsasvqywq
      481 fecwtnltng htadnylvaa atcdawvmwv tantslhffw vgtllacqcy qimvlgmttn
      541 ermnagryah fkqgnpfhrg aiqnaadfcn isfcgikakp ssdwlhsfdh kqsieklpll
      601 atkdnfqyi
//
LOCUS       XP_034196208             353 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611890 [Osmia lignaria lignaria].
ACCESSION   XP_034196208
VERSION     XP_034196208.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340317.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="uncharacterized protein LOC117611890"
                     /calculated_mol_wt=40134
     CDS             1..353
                     /gene="LOC117611890"
                     /coded_by="XM_034340317.2:3075..4136"
                     /db_xref="GeneID:117611890"
ORIGIN      
        1 mvsgedctyn dtteasnits lqsdnscvvn tnfkeaefvk kedntfeqfs hkdckcygih
       61 splnhgvvse hcgrpntvqe lpkdlqknqc tmkqiqstap clcrthkina ytlplkhkng
      121 phsrpfwcrf kwkwpkktyl ekkdamyevn lrsskcahyn kpfmyceise gipnkrkqnp
      181 pkvvkpeeke kgkeikvtek ieiyyfdhgn sayyrttdsp pilatekcae ktdqaatrfw
      241 aeifgtihig tafvtafilq flrfilysvi rpltvgilql ladyfikpll siifnaliqp
      301 vlillyniat slrdlcepia eamglflrel anvcaairiv evkqgtapsa vct
//
LOCUS       XP_034196209             320 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611891 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196209
VERSION     XP_034196209.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340318.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196209.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="uncharacterized protein LOC117611891 isoform X1"
                     /calculated_mol_wt=35872
     Region          195..>279
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          214..236
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(221,223,225,227..228,231..232,235,251,253,257..258,
                     261..262,265,279,281,283,285..286,289..290,293)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <238..292
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          244..266
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          258..283
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          274..294
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..320
                     /gene="LOC117611891"
                     /coded_by="XM_034340318.2:496..1458"
                     /db_xref="GeneID:117611891"
ORIGIN      
        1 miedaamydm tdellfsalg lttdtgverq lftswntvsc dyepsscrtt phsdysetid
       61 shsnnfanpi qcytdltcpt ktswnewsdn tstslsgcls elseldsele wcldkswnsg
      121 lpertplcta gcegflhlpl pnpqqtfqhe eplwvlgidl ralddtmets gidynnnqve
      181 enlissaaaa aalathdytn rnfanaaedr cfpctyqgcv kvyakashlk ahlrrhtgek
      241 pfactwsgcg wrfsrsdela rhrrshsgvk pypcemcskr farsdhlakh rkvhrknayp
      301 lfhagrglrg gkmnivptei
//
LOCUS       XP_034196210             311 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611891 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196210
VERSION     XP_034196210.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340319.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196210.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="uncharacterized protein LOC117611891 isoform X2"
                     /calculated_mol_wt=34832
     Region          186..>270
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          205..227
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(212,214,216,218..219,222..223,226,242,244,248..249,
                     252..253,256,270,272,274,276..277,280..281,284)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          <229..283
                     /region_name="SFP1"
                     /note="Putative transcriptional repressor regulating G2/M
                     transition [Transcription / Cell division and chromosome
                     partitioning]; COG5189"
                     /db_xref="CDD:227516"
     Region          235..257
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          249..274
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          265..285
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..311
                     /gene="LOC117611891"
                     /coded_by="XM_034340319.2:599..1534"
                     /db_xref="GeneID:117611891"
ORIGIN      
        1 mtdellfsal glttdtgver qlftswntvs cdyepsscrt tphsdyseti dshsnnfanp
       61 iqcytdltcp tktswnewsd ntstslsgcl selseldsel ewcldkswns glpertplct
      121 agcegflhlp lpnpqqtfqh eeplwvlgid lralddtmet sgidynnnqv eenlissaaa
      181 aaalathdyt nrnfanaaed rcfpctyqgc vkvyakashl kahlrrhtge kpfactwsgc
      241 gwrfsrsdel arhrrshsgv kpypcemcsk rfarsdhlak hrkvhrknay plfhagrglr
      301 ggkmnivpte i
//
LOCUS       XP_034196211             294 aa            linear   INV 03-SEP-2025
DEFINITION  MOB kinase activator 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196211
VERSION     XP_034196211.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340320.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="MOB kinase activator 2 isoform X1"
                     /calculated_mol_wt=32310
     Region          101..263
                     /region_name="Mob1_phocein"
                     /note="Mob1/phocein family; pfam03637"
                     /db_xref="CDD:461000"
     CDS             1..294
                     /gene="Mob2"
                     /coded_by="XM_034340320.2:1067..1951"
                     /db_xref="GeneID:117611892"
ORIGIN      
        1 mlrsparvvl qdssastsst lsapptpvhh gaasgsagta sgssssvssr lslldtchrk
       61 irffgelvak arrkekdagt tedpklylee aalerqlpel dlrmlvdlpp gldynewlas
      121 htlalfdhin lvygtisefc tmtgcpdmtg pglrtylwfd ekgkktrvaa pqyidyvmtf
      181 tqrtvsdeti fptkyanefp ssfesivrki lrllyhvvah iyhchfreva llglhahlnc
      241 vfahltllnq rfnlidpket eilgdleaal lgdstsssap sqtlaiqetp ttat
//
LOCUS       XP_034196212             294 aa            linear   INV 03-SEP-2025
DEFINITION  MOB kinase activator 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196212
VERSION     XP_034196212.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340321.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="MOB kinase activator 2 isoform X1"
                     /calculated_mol_wt=32310
     Region          101..263
                     /region_name="Mob1_phocein"
                     /note="Mob1/phocein family; pfam03637"
                     /db_xref="CDD:461000"
     CDS             1..294
                     /gene="Mob2"
                     /coded_by="XM_034340321.2:565..1449"
                     /db_xref="GeneID:117611892"
ORIGIN      
        1 mlrsparvvl qdssastsst lsapptpvhh gaasgsagta sgssssvssr lslldtchrk
       61 irffgelvak arrkekdagt tedpklylee aalerqlpel dlrmlvdlpp gldynewlas
      121 htlalfdhin lvygtisefc tmtgcpdmtg pglrtylwfd ekgkktrvaa pqyidyvmtf
      181 tqrtvsdeti fptkyanefp ssfesivrki lrllyhvvah iyhchfreva llglhahlnc
      241 vfahltllnq rfnlidpket eilgdleaal lgdstsssap sqtlaiqetp ttat
//
LOCUS       XP_034196213             294 aa            linear   INV 03-SEP-2025
DEFINITION  MOB kinase activator 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196213
VERSION     XP_034196213.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340322.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="MOB kinase activator 2 isoform X1"
                     /calculated_mol_wt=32310
     Region          101..263
                     /region_name="Mob1_phocein"
                     /note="Mob1/phocein family; pfam03637"
                     /db_xref="CDD:461000"
     CDS             1..294
                     /gene="Mob2"
                     /coded_by="XM_034340322.2:572..1456"
                     /db_xref="GeneID:117611892"
ORIGIN      
        1 mlrsparvvl qdssastsst lsapptpvhh gaasgsagta sgssssvssr lslldtchrk
       61 irffgelvak arrkekdagt tedpklylee aalerqlpel dlrmlvdlpp gldynewlas
      121 htlalfdhin lvygtisefc tmtgcpdmtg pglrtylwfd ekgkktrvaa pqyidyvmtf
      181 tqrtvsdeti fptkyanefp ssfesivrki lrllyhvvah iyhchfreva llglhahlnc
      241 vfahltllnq rfnlidpket eilgdleaal lgdstsssap sqtlaiqetp ttat
//
LOCUS       XP_034196214             294 aa            linear   INV 03-SEP-2025
DEFINITION  MOB kinase activator 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196214
VERSION     XP_034196214.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340323.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..294
                     /product="MOB kinase activator 2 isoform X1"
                     /calculated_mol_wt=32310
     Region          101..263
                     /region_name="Mob1_phocein"
                     /note="Mob1/phocein family; pfam03637"
                     /db_xref="CDD:461000"
     CDS             1..294
                     /gene="Mob2"
                     /coded_by="XM_034340323.2:1068..1952"
                     /db_xref="GeneID:117611892"
ORIGIN      
        1 mlrsparvvl qdssastsst lsapptpvhh gaasgsagta sgssssvssr lslldtchrk
       61 irffgelvak arrkekdagt tedpklylee aalerqlpel dlrmlvdlpp gldynewlas
      121 htlalfdhin lvygtisefc tmtgcpdmtg pglrtylwfd ekgkktrvaa pqyidyvmtf
      181 tqrtvsdeti fptkyanefp ssfesivrki lrllyhvvah iyhchfreva llglhahlnc
      241 vfahltllnq rfnlidpket eilgdleaal lgdstsssap sqtlaiqetp ttat
//
LOCUS       XP_034196216             258 aa            linear   INV 03-SEP-2025
DEFINITION  MOB kinase activator 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196216
VERSION     XP_034196216.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340325.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..258
                     /product="MOB kinase activator 2 isoform X2"
                     /calculated_mol_wt=29057
     Region          65..227
                     /region_name="Mob1_phocein"
                     /note="Mob1/phocein family; pfam03637"
                     /db_xref="CDD:461000"
     CDS             1..258
                     /gene="Mob2"
                     /coded_by="XM_034340325.2:302..1078"
                     /db_xref="GeneID:117611892"
ORIGIN      
        1 mfglgnnrkv lkysaidtsf sidvedtvtc fcrkarrkek dagttedpkl yleeaalerq
       61 lpeldlrmlv dlppgldyne wlashtlalf dhinlvygti sefctmtgcp dmtgpglrty
      121 lwfdekgkkt rvaapqyidy vmtftqrtvs detifptkya nefpssfesi vrkilrllyh
      181 vvahiyhchf revallglha hlncvfahlt llnqrfnlid pketeilgdl eaallgdsts
      241 ssapsqtlai qetpttat
//
LOCUS       XP_034196219             102 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611894 [Osmia lignaria lignaria].
ACCESSION   XP_034196219
VERSION     XP_034196219.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340328.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="uncharacterized protein LOC117611894"
                     /calculated_mol_wt=11687
     CDS             1..102
                     /gene="LOC117611894"
                     /coded_by="XM_034340328.2:813..1121"
                     /db_xref="GeneID:117611894"
ORIGIN      
        1 mivgdvnyfs psaressdiy pftstlaner rvassditvk nerkdnekla mnvkikmdrd
       61 trltstlssr sirnpalefw ltklitfdri mgqyvtgyif kr
//
LOCUS       XP_034196390            1503 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034196390
VERSION     XP_034196390.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340499.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196390.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1503
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TARBP1 isoform X1"
                     /calculated_mol_wt=175972
     Region          1352..1496
                     /region_name="SpoU-like_TRM3-like"
                     /note="SAM-dependent tRNA methylase related to TRM3;
                     cd18091"
                     /db_xref="CDD:349964"
     Site            order(1370..1371,1373..1376,1403,1440..1441,1474..1478,
                     1482..1484,1486..1487,1490..1491,1494..1495)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349964"
     Site            order(1431..1433,1453..1457,1459,1472..1474,1476,
                     1481..1483,1485,1488)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349964"
     CDS             1..1503
                     /gene="LOC117611961"
                     /coded_by="XM_034340499.2:1..4512"
                     /db_xref="GeneID:117611961"
ORIGIN      
        1 mdlherymrs tmdfsvfels etafkddpll llhrivkyyn neimkdnfdt kkletfrivl
       61 cyeyqllfns keknikvaas dlcfktiesi lflkkhsqnf tehpilydii tlqlilycnl
      121 qcieehlmnf qksnvdvlnn deskffyiki lecfleslql rhllynnadl silkhfslly
      181 lkdiitwlem kenkewqlft sifpklistc ssenilpiiw nvilnklddf kdllsalsii
      241 vnvcfssnfi ndvihthykl fyteqlwlli ikslkspiqq yrkqglfimk hitnfmnitn
      301 envlklkips ivpfvcgqkt etkilikdik qkfflildal eekqhhlilp altnlstlik
      361 gneehtlcnd cfntiwlqli ferilqhenn nivkqgvlfv cklytfindn qflklfvhvl
      421 nntflyecqs yqdepdilss iiilfihvte eetefpkrll kimseetwap ipifyvikvl
      481 rtvtsktlnq wgddelilvk slvqknlnmh shilriasqv elikticfsi gkvnnleivi
      541 etllefsled tlvresfswe ivvnwlkevl ikkhaidfik ficeqfssqd shvklspakf
      601 avviamfydt glilqhktcv vfgilnnwls ilngintrpy vnhtyifyai efmshlfnln
      661 viktdetiiq llssyfndsl qflfknsksi pykfsyeein kyliiivsfl nnknlilsek
      721 eilnhverfr sqsifilqni kqytnmhymy alhilyhtqe ilykhctlly veslldisni
      781 hmsnndqdsh vqdqvntkgk itsncyllla kllnqflekk eiqlwpqnin wfkstlclye
      841 mggneivpei asilktiink gaikdiedkl hlesiftlcw kstllskknk lyfiaikdlv
      901 gvvindnflk lpdvmhfvnn fldqlleesn nvpklkqill nemeslntcy lknlqeplvs
      961 cclhghvlrr dkqienqayl yvtqnyrnyy prhisiidyn ndnsvralsi illykiinad
     1021 keyasmllpi vlqklekfrn kryfnhsyih kikhrimqil lilqpilnkl diviledclc
     1081 nlmllesnqh sirlmqewil ikifveniel hyklwkffee gittrpgcvs siacivyhvs
     1141 rllpsdsqcd filmgikyia hcclgqqysm rlynqiifvk lykmleqfnc ndtivqykgl
     1201 ydaaiaslky gnlainsski qddfyfsvfh avhdfslqti yfelprltdm dpsewitpdl
     1261 fqilnfketi nhplqlynln tllsdtktss ylmkstvdke ssvknsmmdi eelnniqkki
     1321 dplmptnish ndifstires vshkrlsded glivvaclvn rapnlggltr tceifnvkel
     1381 vianlnqikd kefqnlsvsa enwititevk phqlckflld kkemgwslvg leqtvnsvnl
     1441 inmkfkkkti lvlgnekdgi panlislfdt cieipqagii rslnvhvsga iciwqyskqh
     1501 vli
//
LOCUS       XP_034196392            1223 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611961 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196392
VERSION     XP_034196392.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340501.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196392.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1223
                     /product="uncharacterized protein LOC117611961 isoform X2"
                     /calculated_mol_wt=142970
     Region          1072..1216
                     /region_name="SpoU-like_TRM3-like"
                     /note="SAM-dependent tRNA methylase related to TRM3;
                     cd18091"
                     /db_xref="CDD:349964"
     Site            order(1090..1091,1093..1096,1123,1160..1161,1194..1198,
                     1202..1204,1206..1207,1210..1211,1214..1215)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349964"
     Site            order(1151..1153,1173..1177,1179,1192..1194,1196,
                     1201..1203,1205,1208)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349964"
     CDS             1..1223
                     /gene="LOC117611961"
                     /coded_by="XM_034340501.2:1..3672"
                     /db_xref="GeneID:117611961"
ORIGIN      
        1 mdlherymrs tmdfsvfels etafkddpll llhrivkyyn neimkdnfdt kkletfrivl
       61 cyeyqllfns keknikvaas dlcfktiesi lflkkhsqnf tehpilydii tlqlilycnl
      121 qcieehlmnf qksnvdvlnn deskffyiki lecfleslql rhllynnadl silkhfslly
      181 lkdiitwlem kenkewqlft sifpklistc ssenilpiiw nvilnklddf kdllsalsii
      241 vnvcfssnfi ndvihthykl fyteqlwlli ikslkspiqq yrkqglfimk hitnfmnitn
      301 envlklkips ivpfvcgqkt etkilikdik qkfflildal eekqhhlilp altnlstlik
      361 gneehtlcnd cfntiwlqli ferilqhenn nivkqgvlfv cklytfindn qflklfvhvl
      421 nntflyecqs yqdepdilss iiilfihvte eetefpkrll kimseetwap ipifyvikvl
      481 rtvtsktlnq wgddelilvk slvqknlnmh shilriasqv elikticfsi gkvnnleivi
      541 etllefsled tlvresfswe ivvnwlkevl ikkhaidfik ficeqfssqd shvklspakf
      601 avviamfydt glilqhktcv vfgilnnwls ilngintrpy fldqlleesn nvpklkqill
      661 nemeslntcy lknlqeplvs cclhghvlrr dkqienqayl yvtqnyrnyy prhisiidyn
      721 ndnsvralsi illykiinad keyasmllpi vlqklekfrn kryfnhsyih kikhrimqil
      781 lilqpilnkl diviledclc nlmllesnqh sirlmqewil ikifveniel hyklwkffee
      841 gittrpgcvs siacivyhvs rllpsdsqcd filmgikyia hcclgqqysm rlynqiifvk
      901 lykmleqfnc ndtivqykgl ydaaiaslky gnlainsski qddfyfsvfh avhdfslqti
      961 yfelprltdm dpsewitpdl fqilnfketi nhplqlynln tllsdtktss ylmkstvdke
     1021 ssvknsmmdi eelnniqkki dplmptnish ndifstires vshkrlsded glivvaclvn
     1081 rapnlggltr tceifnvkel vianlnqikd kefqnlsvsa enwititevk phqlckflld
     1141 kkemgwslvg leqtvnsvnl inmkfkkkti lvlgnekdgi panlislfdt cieipqagii
     1201 rslnvhvsga iciwqyskqh vli
//
LOCUS       XP_034196393            1196 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034196393
VERSION     XP_034196393.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340502.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196393.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1196
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TARBP1 isoform X3"
                     /calculated_mol_wt=139437
     Region          1045..1189
                     /region_name="SpoU-like_TRM3-like"
                     /note="SAM-dependent tRNA methylase related to TRM3;
                     cd18091"
                     /db_xref="CDD:349964"
     Site            order(1063..1064,1066..1069,1096,1133..1134,1167..1171,
                     1175..1177,1179..1180,1183..1184,1187..1188)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349964"
     Site            order(1124..1126,1146..1150,1152,1165..1167,1169,
                     1174..1176,1178,1181)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349964"
     CDS             1..1196
                     /gene="LOC117611961"
                     /coded_by="XM_034340502.2:1..3591"
                     /db_xref="GeneID:117611961"
ORIGIN      
        1 mhiyvyilkn prahwlsiyi csaaynqqak yaleekqhhl ilpaltnlst likgneehtl
       61 cndcfntiwl qliferilqh ennnivkqgv lfvcklytfi ndnqflklfv hvlnntflye
      121 cqsyqdepdi lssiiilfih vteeetefpk rllkimseet wapipifyvi kvlrtvtskt
      181 lnqwgddeli lvkslvqknl nmhshilria sqveliktic fsigkvnnle ivietllefs
      241 ledtlvresf sweivvnwlk evlikkhaid fikficeqfs sqdshvklsp akfavviamf
      301 ydtglilqhk tcvvfgilnn wlsilngint rpyvnhtyif yaiefmshlf nlnviktdet
      361 iiqllssyfn dslqflfkns ksipykfsye einkyliiiv sflnnknlil sekeilnhve
      421 rfrsqsifil qnikqytnmh ymyalhilyh tqeilykhct llyveslldi snihmsnndq
      481 dshvqdqvnt kgkitsncyl llakllnqfl ekkeiqlwpq ninwfkstlc lyemggneiv
      541 peiasilkti inkgaikdie dklhlesift lcwkstllsk knklyfiaik dlvgvvindn
      601 flklpdvmhf vnnfldqlle esnnvpklkq illnemesln tcylknlqep lvscclhghv
      661 lrrdkqienq aylyvtqnyr nyyprhisii dynndnsvra lsiillykii nadkeyasml
      721 lpivlqklek frnkryfnhs yihkikhrim qillilqpil nkldiviled clcnlmlles
      781 nqhsirlmqe wilikifven ielhyklwkf feegittrpg cvssiacivy hvsrllpsds
      841 qcdfilmgik yiahcclgqq ysmrlynqii fvklykmleq fncndtivqy kglydaaias
      901 lkygnlains skiqddfyfs vfhavhdfsl qtiyfelprl tdmdpsewit pdlfqilnfk
      961 etinhplqly nlntllsdtk tssylmkstv dkessvknsm mdieelnniq kkidplmptn
     1021 ishndifsti resvshkrls dedglivvac lvnrapnlgg ltrtceifnv kelvianlnq
     1081 ikdkefqnls vsaenwitit evkphqlckf lldkkemgws lvgleqtvns vnlinmkfkk
     1141 ktilvlgnek dgipanlisl fdtcieipqa giirslnvhv sgaiciwqys kqhvli
//
LOCUS       XP_034196394            1194 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1 isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034196394
VERSION     XP_034196394.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340503.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196394.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1194
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TARBP1 isoform X4"
                     /calculated_mol_wt=140972
     CDS             1..1194
                     /gene="LOC117611961"
                     /coded_by="XM_034340503.2:7..3591"
                     /db_xref="GeneID:117611961"
ORIGIN      
        1 mdlherymrs tmdfsvfels etafkddpll llhrivkyyn neimkdnfdt kkletfrivl
       61 cyeyqllfns keknikvaas dlcfktiesi lflkkhsqnf tehpilydii tlqlilycnl
      121 qcieehlmnf qksnvdvlnn deskffyiki lecfleslql rhllynnadl silkhfslly
      181 lkdiitwlem kenkewqlft sifpklistc ssenilpiiw nvilnklddf kdllsalsii
      241 vnvcfssnfi ndvihthykl fyteqlwlli ikslkspiqq yrkqglfimk hitnfmnitn
      301 envlklkips ivpfvcgqkt etkilikdik qkfflildal eekqhhlilp altnlstlik
      361 gneehtlcnd cfntiwlqli ferilqhenn nivkqgvlfv cklytfindn qflklfvhvl
      421 nntflyecqs yqdepdilss iiilfihvte eetefpkrll kimseetwap ipifyvikvl
      481 rtvtsktlnq wgddelilvk slvqknlnmh shilriasqv elikticfsi gkvnnleivi
      541 etllefsled tlvresfswe ivvnwlkevl ikkhaidfik ficeqfssqd shvklspakf
      601 avviamfydt glilqhktcv vfgilnnwls ilngintrpy vnhtyifyai efmshlfnln
      661 viktdetiiq llssyfndsl qflfknsksi pykfsyeein kyliiivsfl nnknlilsek
      721 eilnhverfr sqsifilqni kqytnmhymy alhilyhtqe ilykhctlly veslldisni
      781 hmsnndqdsh vqdqvntkgk itsncyllla kllnqflekk eiqlwpqnin wfkstlclye
      841 mggneivpei asilktiink gaikdiedkl hlesiftlcw kstllskknk lyfiaikdlv
      901 gvvindnflk lpdvmhfvnn fldqlleesn nvpklkqill nemeslntcy lknlqeplvs
      961 cclhghvlrr dkqienqayl yvtqnyrnyy prhisiidyn ndnsvralsi illykiinad
     1021 keyasmllpi vlqklekfrn kryfnhsyih kikhrimqil lilqpilnkl diviledclc
     1081 nlmllesnqh sirlmqewil ikifveniel hyklwkffee gittrpgcvs siacivyhvs
     1141 rllpsdsqcd filmgikyia hcclgqqysm rlynqffmlc mtlvyrqfil nsrd
//
LOCUS       XP_034196395            1015 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA (guanosine(18)-2'-O)-methyltransferase TARBP1 isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034196395
VERSION     XP_034196395.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340504.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196395.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1015
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TARBP1 isoform X5"
                     /calculated_mol_wt=118515
     Region          864..1008
                     /region_name="SpoU-like_TRM3-like"
                     /note="SAM-dependent tRNA methylase related to TRM3;
                     cd18091"
                     /db_xref="CDD:349964"
     Site            order(882..883,885..888,915,952..953,986..990,994..996,
                     998..999,1002..1003,1006..1007)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:349964"
     Site            order(943..945,965..969,971,984..986,988,993..995,997,
                     1000)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349964"
     CDS             1..1015
                     /gene="LOC117611961"
                     /coded_by="XM_034340504.2:1..3048"
                     /db_xref="GeneID:117611961"
ORIGIN      
        1 mdlherymrs tmdfsvfels etafkddpll llhrivkyyn neimkdnfdt kkletfrivl
       61 cyeyqllfns keknikvaas dlcfktiesi lflkkhsqnf tehpilydii tlqlilycnl
      121 qcieehlmnf qksnvdvlnn deskffyiki lecfleslql rhllynnadl silkhfslly
      181 lkdiitwlem kenkewqlft sifpklistc ssenilpiiw nvilnklddf kdllsalsii
      241 vnvcfssnfi ndvihthykl fyteqlwlli ikslkspiqq yrkqglfimk hitnfmnitn
      301 envlklkips ivpfvcgqkt etkilikdik qkfflildal eekqhhlilp altnlstlik
      361 eiasilktii nkgaikdied klhlesiftl cwkstllskk nklyfiaikd lvgvvindnf
      421 lklpdvmhfv nnfldqllee snnvpklkqi llnemeslnt cylknlqepl vscclhghvl
      481 rrdkqienqa ylyvtqnyrn yyprhisiid ynndnsvral siillykiin adkeyasmll
      541 pivlqklekf rnkryfnhsy ihkikhrimq illilqpiln kldiviledc lcnlmllesn
      601 qhsirlmqew ilikifveni elhyklwkff eegittrpgc vssiacivyh vsrllpsdsq
      661 cdfilmgiky iahcclgqqy smrlynqiif vklykmleqf ncndtivqyk glydaaiasl
      721 kygnlainss kiqddfyfsv fhavhdfslq tiyfelprlt dmdpsewitp dlfqilnfke
      781 tinhplqlyn lntllsdtkt ssylmkstvd kessvknsmm dieelnniqk kidplmptni
      841 shndifstir esvshkrlsd edglivvacl vnrapnlggl trtceifnvk elvianlnqi
      901 kdkefqnlsv saenwitite vkphqlckfl ldkkemgwsl vgleqtvnsv nlinmkfkkk
      961 tilvlgnekd gipanlislf dtcieipqag iirslnvhvs gaiciwqysk qhvli
//
LOCUS       XP_034194694             825 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (2) giant discs 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194694
VERSION     XP_034194694.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338803.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194694.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..825
                     /product="lethal (2) giant discs 1 isoform X1"
                     /calculated_mol_wt=91699
     Region          142..202
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          251..309
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          <301..451
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          354..411
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          488..545
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          654..823
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..825
                     /gene="l(2)gd1"
                     /coded_by="XM_034338803.2:155..2632"
                     /db_xref="GeneID:117610948"
ORIGIN      
        1 mfgkkekskr qtrpagslaq ygifdipenl dnignefmed ddnnddleae lealtsgdds
       61 vskpkqtark pvpkatldam iaesikdinv eevsdgeddp ellselkmit gdesdekvsp
      121 eeeenteqsk sqqddtpmqn titllqerlk lyqiaekkak qendlgrarr fnrgiktlke
      181 llhnvqagrv inegdippel ppsataesie taskndeskp ennvaskvva siesntpeep
      241 mptktvdeea lnllidrqre yklaavawkr agnmkealqc isvvkqfdkv iavvnagetv
      301 dlsdmpsspn tpgsnttavs pekteriene mqgtssseta stepkpagle nvetalkerl
      361 evyrrmktaa esegnsskvr rygrickqfe dalklhtrgk pvpldelptp pgfpplslgp
      421 qssastpave knenqpapip esipetipes epaenkidss pkspvspprr qggkkqigkq
      481 tradkqsall qqrqhelkqa alnakkegdl elarnylrqa kgidllieas taglpvdmns
      541 iplsprakfe lsgdsitgqt ddsfivlnne dfeegtatde eiyenlesql tkqikwclct
      601 rdhskalgdv pgynrwerla lgykrdldml rvrkrdalps pqhhyetkty aivqsctdls
      661 dadieisiir gvnyskegdt yvmfefpyps dnppsdrtit vkgtcnpeyq avfpltgiid
      721 rssrqcqraf krhalkcqvw akgcslnpfl cctnsrgffr sdsllgtvtv klqpletqce
      781 lhdsfplmdg rkatggklel kirlrnpilt kqieqitdkw ltidy
//
LOCUS       XP_034194698             824 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (2) giant discs 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194698
VERSION     XP_034194698.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338807.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194698.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..824
                     /product="lethal (2) giant discs 1 isoform X2"
                     /calculated_mol_wt=91628
     Region          141..201
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          250..308
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          <300..450
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          353..410
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          487..544
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          653..822
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..824
                     /gene="l(2)gd1"
                     /coded_by="XM_034338807.2:155..2629"
                     /db_xref="GeneID:117610948"
ORIGIN      
        1 mfgkkekskr qtrpagslaq ygifdipenl dnignefmed ddnnddleae lealtsgdds
       61 vskpkqtrkp vpkatldami aesikdinve evsdgeddpe llselkmitg desdekvspe
      121 eeenteqsks qqddtpmqnt itllqerlkl yqiaekkakq endlgrarrf nrgiktlkel
      181 lhnvqagrvi negdippelp psataesiet askndeskpe nnvaskvvas iesntpeepm
      241 ptktvdeeal nllidrqrey klaavawkra gnmkealqci svvkqfdkvi avvnagetvd
      301 lsdmpsspnt pgsnttavsp ekterienem qgtsssetas tepkpaglen vetalkerle
      361 vyrrmktaae segnsskvrr ygrickqfed alklhtrgkp vpldelptpp gfpplslgpq
      421 ssastpavek nenqpapipe sipetipese paenkidssp kspvspprrq ggkkqigkqt
      481 radkqsallq qrqhelkqaa lnakkegdle larnylrqak gidllieast aglpvdmnsi
      541 plsprakfel sgdsitgqtd dsfivlnned feegtatdee iyenlesqlt kqikwclctr
      601 dhskalgdvp gynrwerlal gykrdldmlr vrkrdalpsp qhhyetktya ivqsctdlsd
      661 adieisiirg vnyskegdty vmfefpypsd nppsdrtitv kgtcnpeyqa vfpltgiidr
      721 ssrqcqrafk rhalkcqvwa kgcslnpflc ctnsrgffrs dsllgtvtvk lqpletqcel
      781 hdsfplmdgr katggklelk irlrnpiltk qieqitdkwl tidy
//
LOCUS       XP_034194704             812 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (2) giant discs 1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034194704
VERSION     XP_034194704.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338813.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194704.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..812
                     /product="lethal (2) giant discs 1 isoform X3"
                     /calculated_mol_wt=90260
     Region          142..202
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          251..309
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          <301..451
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          354..411
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          488..545
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          654..810
                     /region_name="C2_Freud-1"
                     /note="C2 domain found in 5' repressor element under dual
                     repression binding protein-1 (Freud-1); cd08690"
                     /db_xref="CDD:176072"
     Site            order(679,741,743,749,752)
                     /site_type="other"
                     /note="putative Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:176072"
     CDS             1..812
                     /gene="l(2)gd1"
                     /coded_by="XM_034338813.2:153..2591"
                     /db_xref="GeneID:117610948"
ORIGIN      
        1 mfgkkekskr qtrpagslaq ygifdipenl dnignefmed ddnnddleae lealtsgdds
       61 vskpkqtark pvpkatldam iaesikdinv eevsdgeddp ellselkmit gdesdekvsp
      121 eeeenteqsk sqqddtpmqn titllqerlk lyqiaekkak qendlgrarr fnrgiktlke
      181 llhnvqagrv inegdippel ppsataesie taskndeskp ennvaskvva siesntpeep
      241 mptktvdeea lnllidrqre yklaavawkr agnmkealqc isvvkqfdkv iavvnagetv
      301 dlsdmpsspn tpgsnttavs pekteriene mqgtssseta stepkpagle nvetalkerl
      361 evyrrmktaa esegnsskvr rygrickqfe dalklhtrgk pvpldelptp pgfpplslgp
      421 qssastpave knenqpapip esipetipes epaenkidss pkspvspprr qggkkqigkq
      481 tradkqsall qqrqhelkqa alnakkegdl elarnylrqa kgidllieas taglpvdmns
      541 iplsprakfe lsgdsitgqt ddsfivlnne dfeegtatde eiyenlesql tkqikwclct
      601 rdhskalgdv pgynrwerla lgykrdldml rvrkrdalps pqhhyetkty aivqsctdls
      661 dadieisiir gvnyskegdt yvmfefpyps dnppsdrtit vkgtcnpeyq avfpltgiid
      721 rssrqcqraf krhalkcqvw akggffrsds llgtvtvklq pletqcelhd sfplmdgrka
      781 tggklelkir lrnpiltkqi eqitdkwlti dy
//
LOCUS       XP_034194709             746 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (2) giant discs 1 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034194709
VERSION     XP_034194709.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338818.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194709.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..746
                     /product="lethal (2) giant discs 1 isoform X4"
                     /calculated_mol_wt=83004
     Region          63..123
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          172..230
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          <222..372
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          275..332
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          409..466
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          575..744
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..746
                     /gene="l(2)gd1"
                     /coded_by="XM_034338818.2:181..2421"
                     /db_xref="GeneID:117610948"
ORIGIN      
        1 miaesikdin veevsdgedd pellselkmi tgdesdekvs peeeenteqs ksqqddtpmq
       61 ntitllqerl klyqiaekka kqendlgrar rfnrgiktlk ellhnvqagr vinegdippe
      121 lppsataesi etaskndesk pennvaskvv asiesntpee pmptktvdee alnllidrqr
      181 eyklaavawk ragnmkealq cisvvkqfdk viavvnaget vdlsdmpssp ntpgsnttav
      241 spekterien emqgtssset astepkpagl envetalker levyrrmkta aesegnsskv
      301 rrygrickqf edalklhtrg kpvpldelpt ppgfpplslg pqssastpav eknenqpapi
      361 pesipetipe sepaenkids spkspvsppr rqggkkqigk qtradkqsal lqqrqhelkq
      421 aalnakkegd lelarnylrq akgidlliea staglpvdmn siplsprakf elsgdsitgq
      481 tddsfivlnn edfeegtatd eeiyenlesq ltkqikwclc trdhskalgd vpgynrwerl
      541 algykrdldm lrvrkrdalp spqhhyetkt yaivqsctdl sdadieisii rgvnyskegd
      601 tyvmfefpyp sdnppsdrti tvkgtcnpey qavfpltgii drssrqcqra fkrhalkcqv
      661 wakgcslnpf lcctnsrgff rsdsllgtvt vklqpletqc elhdsfplmd grkatggkle
      721 lkirlrnpil tkqieqitdk wltidy
//
LOCUS       XP_034194716             811 aa            linear   INV 03-SEP-2025
DEFINITION  lethal (2) giant discs 1 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034194716
VERSION     XP_034194716.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338825.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194716.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..811
                     /product="lethal (2) giant discs 1 isoform X5"
                     /calculated_mol_wt=90188
     Region          141..201
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          250..308
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          <300..450
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     Region          353..410
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          487..544
                     /region_name="DM14"
                     /note="Repeats in fly CG4713, worm Y37H9A.3 and human
                     FLJ20241; smart00685"
                     /db_xref="CDD:128928"
     Region          653..809
                     /region_name="C2_Freud-1"
                     /note="C2 domain found in 5' repressor element under dual
                     repression binding protein-1 (Freud-1); cd08690"
                     /db_xref="CDD:176072"
     Site            order(678,740,742,748,751)
                     /site_type="other"
                     /note="putative Ca2+ binding pocket [ion binding]"
                     /db_xref="CDD:176072"
     CDS             1..811
                     /gene="l(2)gd1"
                     /coded_by="XM_034338825.2:153..2588"
                     /db_xref="GeneID:117610948"
ORIGIN      
        1 mfgkkekskr qtrpagslaq ygifdipenl dnignefmed ddnnddleae lealtsgdds
       61 vskpkqtrkp vpkatldami aesikdinve evsdgeddpe llselkmitg desdekvspe
      121 eeenteqsks qqddtpmqnt itllqerlkl yqiaekkakq endlgrarrf nrgiktlkel
      181 lhnvqagrvi negdippelp psataesiet askndeskpe nnvaskvvas iesntpeepm
      241 ptktvdeeal nllidrqrey klaavawkra gnmkealqci svvkqfdkvi avvnagetvd
      301 lsdmpsspnt pgsnttavsp ekterienem qgtsssetas tepkpaglen vetalkerle
      361 vyrrmktaae segnsskvrr ygrickqfed alklhtrgkp vpldelptpp gfpplslgpq
      421 ssastpavek nenqpapipe sipetipese paenkidssp kspvspprrq ggkkqigkqt
      481 radkqsallq qrqhelkqaa lnakkegdle larnylrqak gidllieast aglpvdmnsi
      541 plsprakfel sgdsitgqtd dsfivlnned feegtatdee iyenlesqlt kqikwclctr
      601 dhskalgdvp gynrwerlal gykrdldmlr vrkrdalpsp qhhyetktya ivqsctdlsd
      661 adieisiirg vnyskegdty vmfefpypsd nppsdrtitv kgtcnpeyqa vfpltgiidr
      721 ssrqcqrafk rhalkcqvwa kggffrsdsl lgtvtvklqp letqcelhds fplmdgrkat
      781 ggklelkirl rnpiltkqie qitdkwltid y
//
LOCUS       XP_034196402             604 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 26 [Osmia lignaria lignaria].
ACCESSION   XP_034196402
VERSION     XP_034196402.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340511.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..604
                     /product="WD repeat-containing protein 26"
                     /calculated_mol_wt=67523
     Region          68..96
                     /region_name="LisH_TPL"
                     /note="LisH-like dimerization domain; pfam17814"
                     /db_xref="CDD:375350"
     Region          105..159
                     /region_name="CTLH"
                     /note="C-terminal to LisH motif; smart00668"
                     /db_xref="CDD:128914"
     Region          221..>312
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          <274..571
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          279..327
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          332..372
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          380..414
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          418..463
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          472..540
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          546..571
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..604
                     /gene="LOC117611963"
                     /coded_by="XM_034340511.2:443..2257"
                     /db_xref="GeneID:117611963"
ORIGIN      
        1 menyrimqqq tsnggissgl qqngstadsg gphangnvmp esdndingvn getnqnlgpp
       61 kimdktnqdi vrligqhlkt vglnrtadll mqesgcrldh paaakfrqhv mdgdwtkaeh
      121 dlselktfln ganqslvemk fllleqkyle yleeglvlea lqvlrneltp lghntgrvhq
      181 lsafmmcsgr delqtragwd gkgavsraal mdrlqkylpp simlpprrlh sllcqavemq
      241 nqqctyhvth tqtslenvsl lvdhscskeq fpchtiqvln nhcdevwyck fspdgrklat
      301 gskdmtviiw dvdpetlkvt hrktleghty gvaliawspd sslliacgpe dcpelwlwsi
      361 dppdyelrat vtqntddslt acawypdsrk fvagglrgqf yqfdiegnvs eswegvrvkc
      421 lwckndgktv laadshhrir gynfddlcdf tilqedhplm sfsvnkadrl allnvanqgv
      481 hlwdlqdrcl vrrfqgvtqg hftihscfgg vnqdfiasgs edtkvyvwhi krelpiailt
      541 ghsrtvncvs wnpvyhqmma svsddctvri wgprssspek gesdktaple snssngsgwh
      601 emvs
//
LOCUS       XP_034194728             519 aa            linear   INV 03-SEP-2025
DEFINITION  caspase-8 Dredd [Osmia lignaria lignaria].
ACCESSION   XP_034194728
VERSION     XP_034194728.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338837.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194728.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="caspase-8 Dredd"
                     /calculated_mol_wt=59815
     Region          258..462
                     /region_name="CASc"
                     /note="Caspase, interleukin-1 beta converting enzyme (ICE)
                     homologues; Cysteine-dependent aspartate-directed
                     proteases that mediate programmed cell death (apoptosis).
                     Caspases are synthesized as inactive zymogens and
                     activated by proteolysis of the peptide...; cl00042"
                     /db_xref="CDD:444667"
     Site            order(276,334,371,378,414..419,423..424)
                     /site_type="other"
                     /note="substrate pocket [chemical binding]"
                     /db_xref="CDD:237997"
     Site            order(333,373)
                     /site_type="active"
                     /db_xref="CDD:237997"
     Site            order(380,398)
                     /site_type="active"
                     /note="proteolytic cleavage site [active]"
                     /db_xref="CDD:237997"
     CDS             1..519
                     /gene="Dredd"
                     /coded_by="XM_034338837.2:122..1681"
                     /db_xref="GeneID:117610970"
ORIGIN      
        1 mcvstdamlc ivsnnineel nndilekigy dldideiisv lflmiknyay sfnevygllq
       61 kckdnrshvl vefikkhpen wknkllealc iiqnrhiirk lgisfkdlel lylpknrlcs
      121 rsvnsvakcl yllcealaen nieylvefvk ndlpeyeenf kdtdflelhi lywiqrkyis
      181 idldgkvnlk nllkhlkrfd dldiiyedlk ryednqnvld vqytsstavs qlqmfstred
      241 vhmlsdikeq gikklknglc iiisqmvfmg qkyetrfgnl adcnklsetf ggfnfrvevf
      301 kslkkdemlk kledipkkfg idydcmflci lshgckggii tsdeetisie tiehkicspe
      361 laevikivvi qacqgevagr vddglstdgp tncapnilar knfclfmsti qnfvsvrhkt
      421 egswfiqefc nilqegggki tfltavtkti gsviqkrgkl ngtdsiaqla elrscrlvtd
      481 fllpeyqark knnlhrftfa keenhykltf kwelfnnnl
//
LOCUS       XP_076546811             455 aa            linear   INV 03-SEP-2025
DEFINITION  maf family bZIP transcription factor traffic jam isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546811
VERSION     XP_076546811.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690696.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..455
                     /product="maf family bZIP transcription factor traffic jam
                     isoform X1"
                     /calculated_mol_wt=51101
     Region          <57..>204
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          313..385
                     /region_name="bZIP_Maf_large"
                     /note="Basic leucine zipper (bZIP) domain of large
                     musculoaponeurotic fibrosarcoma (Maf) proteins: a
                     DNA-binding and dimerization domain; cd14718"
                     /db_xref="CDD:269866"
     Region          313..385
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269866"
     Site            order(323,326..328,330..332,334..336,338..339,341..342)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269866"
     Site            order(342,349,352..353,356..357,359..360,363..364,
                     366..367,370,373..374,377..378,380..381)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269866"
     CDS             1..455
                     /gene="tj"
                     /coded_by="XM_076690696.1:2139..3506"
                     /db_xref="GeneID:117610976"
ORIGIN      
        1 meaedhlare yvqefvldhl dpadvkrevr isspmmvngs vvqlpgqvqa qglalapltp
       61 paheleqphp lygqphiqvq hgvlvkappg tashlttlsh pgtppdtppv sasppplqlh
      121 rgerdprerg lflhlqqpns ivqdemqpgt ggmgwltqtl rqepldlrph cpqeqtpeph
      181 heawpstpth hhfqelqqlq rhtrhtggyi tmsghieyyg ganagggmlp aggsgmsgme
      241 dsmqgipmqp grpmsvcsvs scgaggpspa hrtgnglysn casnnpqeel mndellmsls
      301 vrelnkrlhg cpreevsqvv rlkqkrrtlk nrgyaqncrs krlqqrhdle ttnrnlqnel
      361 qrvkielarv qqerdlykqr ceilrtrqnh nhnhnhnhhq qqasqqqqpq pqqhqtpnqq
      421 qqqsqqqqqq qqqpppqqqq qqqhqpapas pevyl
//
LOCUS       XP_034194743             455 aa            linear   INV 03-SEP-2025
DEFINITION  maf family bZIP transcription factor traffic jam isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194743
VERSION     XP_034194743.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338852.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194743.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..455
                     /product="maf family bZIP transcription factor traffic jam
                     isoform X1"
                     /calculated_mol_wt=51101
     Region          <57..>204
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          313..385
                     /region_name="bZIP_Maf_large"
                     /note="Basic leucine zipper (bZIP) domain of large
                     musculoaponeurotic fibrosarcoma (Maf) proteins: a
                     DNA-binding and dimerization domain; cd14718"
                     /db_xref="CDD:269866"
     Region          313..385
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269866"
     Site            order(323,326..328,330..332,334..336,338..339,341..342)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269866"
     Site            order(342,349,352..353,356..357,359..360,363..364,
                     366..367,370,373..374,377..378,380..381)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269866"
     CDS             1..455
                     /gene="tj"
                     /coded_by="XM_034338852.2:2065..3432"
                     /db_xref="GeneID:117610976"
ORIGIN      
        1 meaedhlare yvqefvldhl dpadvkrevr isspmmvngs vvqlpgqvqa qglalapltp
       61 paheleqphp lygqphiqvq hgvlvkappg tashlttlsh pgtppdtppv sasppplqlh
      121 rgerdprerg lflhlqqpns ivqdemqpgt ggmgwltqtl rqepldlrph cpqeqtpeph
      181 heawpstpth hhfqelqqlq rhtrhtggyi tmsghieyyg ganagggmlp aggsgmsgme
      241 dsmqgipmqp grpmsvcsvs scgaggpspa hrtgnglysn casnnpqeel mndellmsls
      301 vrelnkrlhg cpreevsqvv rlkqkrrtlk nrgyaqncrs krlqqrhdle ttnrnlqnel
      361 qrvkielarv qqerdlykqr ceilrtrqnh nhnhnhnhhq qqasqqqqpq pqqhqtpnqq
      421 qqqsqqqqqq qqqpppqqqq qqqhqpapas pevyl
//
LOCUS       XP_034194751             452 aa            linear   INV 03-SEP-2025
DEFINITION  maf family bZIP transcription factor traffic jam isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194751
VERSION     XP_034194751.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338860.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194751.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="maf family bZIP transcription factor traffic jam
                     isoform X2"
                     /calculated_mol_wt=50787
     Region          <57..>204
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          313..382
                     /region_name="bZIP_Maf_large"
                     /note="Basic leucine zipper (bZIP) domain of large
                     musculoaponeurotic fibrosarcoma (Maf) proteins: a
                     DNA-binding and dimerization domain; cd14718"
                     /db_xref="CDD:269866"
     Region          313..382
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269866"
     Site            order(320,323..325,327..329,331..333,335..336,338..339)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269866"
     Site            order(339,346,349..350,353..354,356..357,360..361,
                     363..364,367,370..371,374..375,377..378)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269866"
     CDS             1..452
                     /gene="tj"
                     /coded_by="XM_034338860.2:1828..3186"
                     /db_xref="GeneID:117610976"
ORIGIN      
        1 meaedhlare yvqefvldhl dpadvkrevr isspmmvngs vvqlpgqvqa qglalapltp
       61 paheleqphp lygqphiqvq hgvlvkappg tashlttlsh pgtppdtppv sasppplqlh
      121 rgerdprerg lflhlqqpns ivqdemqpgt ggmgwltqtl rqepldlrph cpqeqtpeph
      181 heawpstpth hhfqelqqlq rhtrhtggyi tmsghieyyg ganagggmlp aggsgmsgme
      241 dsmqgipmqp grpmsvcsvs scgaggpspa hrtgnglysn casnnpqeel mndellmsls
      301 vrelnkrlhg cpreevvrlk qkrrtlknrg yaqncrskrl qqrhdlettn rnlqnelqrv
      361 kielarvqqe rdlykqrcei lrtrqnhnhn hnhnhhqqqa sqqqqpqpqq hqtpnqqqqq
      421 sqqqqqqqqq pppqqqqqqq hqpapaspev yl
//
LOCUS       XP_076546813             353 aa            linear   INV 03-SEP-2025
DEFINITION  sodium channel protein Nach [Osmia lignaria lignaria].
ACCESSION   XP_076546813
VERSION     XP_076546813.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690698.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..353
                     /product="sodium channel protein Nach"
                     /calculated_mol_wt=41020
     Region          <16..342
                     /region_name="ASC"
                     /note="Amiloride-sensitive sodium channel; pfam00858"
                     /db_xref="CDD:459966"
     CDS             1..353
                     /gene="LOC117610988"
                     /coded_by="XM_076690698.1:528..1589"
                     /db_xref="GeneID:117610988"
ORIGIN      
        1 mkllnellhp gehelnirsn ltrlqvifdm nglsiptvin lvtrscnnll eackwkaeek
       61 dcktifqpsf trygmccsfn yitrekilee pnltphrmts cgyqsglnil lnldpddyha
      121 slvesigtki mlhspfdypd dnaasklipv nkysflivqp demystdnvr qestrycifh
      181 deidamnsen kkkryfipar yslinclvec rasviqakcg cvpyyypqnn arvcnlrdle
      241 cietykywyd tawpgtdmsp nvvsltelnt herpcdchpd cnfytyyvqn signldkriy
      301 ytglsytnyp skgktwknqs vihiffgdlv svqyrrdiry swrhvfgnvs lvk
//
LOCUS       XP_034196404             348 aa            linear   INV 03-SEP-2025
DEFINITION  V-type proton ATPase subunit VhaAC39-1 [Osmia lignaria lignaria].
ACCESSION   XP_034196404
VERSION     XP_034196404.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340513.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..348
                     /product="V-type proton ATPase subunit VhaAC39-1"
                     /calculated_mol_wt=39483
     Region          13..341
                     /region_name="vATP-synt_AC39"
                     /note="ATP synthase (C/AC39) subunit; pfam01992"
                     /db_xref="CDD:426553"
     CDS             1..348
                     /gene="VhaAC39-1"
                     /coded_by="XM_034340513.2:276..1322"
                     /db_xref="GeneID:117611965"
ORIGIN      
        1 mkgcmfnidn gyleglcrgf kcgilqqsdy lnlvqcetle dlklhlagtd ygsflaneps
       61 plsvsviddk lreklvvefq hmrnhsvepl sqfldfitys ymidniilli tgtlhqrpis
      121 elipkchplg sfeqmeaihv aatpaelyna vlvdtplapf fvdciseqdl demnieiirn
      181 tlykayleaf ykfckeiggt tantmceila feadrraiii tinsfgtelg kddraklypr
      241 cgklnpdgla alaraddyeq vkavaeyyae ysalfegagn nsgdktledk ffehevrlnv
      301 haflqqfhfg vfysylklke qecrnivwia ecvaqkhrak idnyipif
//
LOCUS       XP_034196405             347 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA-histidine guanylyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034196405
VERSION     XP_034196405.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340514.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196405.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..347
                     /product="tRNA-histidine guanylyltransferase"
                     /calculated_mol_wt=39898
     Region          36..165
                     /region_name="Thg1"
                     /note="tRNAHis guanylyltransferase; pfam04446"
                     /db_xref="CDD:461314"
     Region          168..266
                     /region_name="Thg1C"
                     /note="Thg1 C terminal domain; pfam14413"
                     /db_xref="CDD:464167"
     CDS             1..347
                     /gene="THG"
                     /coded_by="XM_034340514.2:1..1044"
                     /db_xref="GeneID:117611966"
ORIGIN      
        1 myflttgsnf lkiislvkqv vnsrqisltr makskfeyvk eferddcclp ncwivvridg
       61 rnfskfcdah qftkpndvaa lqlmnraait vmedfkeiil gfgqsdeysf vfrkdtqlhk
      121 rrasklmsiv nslfassyvy hwprffqgke lyyppsfdar vilyptdknl rdylawrqtd
      181 vhvnnlyntc fwnlvlqgkl tpsqaeeklr gtlashknel lfqefginyn kepplfrkgt
      241 tlirklvpdg tgrlkpavvp lvddiiadrf wkenpevigl kslatyqppn lvnnatpspt
      301 anikhinntp pssvpnvnsi kkdahptmsr esdgekmqce sericrr
//
LOCUS       XP_034196406             342 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin-binding cofactor C [Osmia lignaria lignaria].
ACCESSION   XP_034196406
VERSION     XP_034196406.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340515.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..342
                     /product="tubulin-binding cofactor C"
                     /calculated_mol_wt=39091
     Region          13..114
                     /region_name="TBCC_N"
                     /note="Tubulin-specific chaperone C N-terminal domain;
                     pfam16752"
                     /db_xref="CDD:465258"
     Region          200..317
                     /region_name="TBCC"
                     /note="Tubulin binding cofactor C; pfam07986"
                     /db_xref="CDD:462331"
     CDS             1..342
                     /gene="TBCC"
                     /coded_by="XM_034340515.2:460..1488"
                     /db_xref="GeneID:117611967"
ORIGIN      
        1 mdsptliegn lpdritkrdr erkniierrr eerqslaves eqssyfkdtf yssckkvkem
       61 ldnapsapts alpgmfdkin keilmlknyl sqskmflkvy dirraqenlq vleneasdle
      121 lkllpkkkfg fknrrvvkkt sekthdvtdg lkdlkisegi vngstkqnyk lsskygdsac
      181 mllgkvdeql vldaenvnkn dvllsdlirc tiriygtpst lhmvnlkqct vlvgpvtssv
      241 fahdcnecvf alacqqlrlh sstdctiylh vtsraiiedc tkirvapynw syedqanhfn
      301 lagldpkinn wnciddfnwl snekhspnws ildpelrvks wd
//
LOCUS       XP_034196407             342 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin-binding cofactor C [Osmia lignaria lignaria].
ACCESSION   XP_034196407
VERSION     XP_034196407.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340516.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..342
                     /product="tubulin-binding cofactor C"
                     /calculated_mol_wt=39091
     Region          13..114
                     /region_name="TBCC_N"
                     /note="Tubulin-specific chaperone C N-terminal domain;
                     pfam16752"
                     /db_xref="CDD:465258"
     Region          200..317
                     /region_name="TBCC"
                     /note="Tubulin binding cofactor C; pfam07986"
                     /db_xref="CDD:462331"
     CDS             1..342
                     /gene="TBCC"
                     /coded_by="XM_034340516.2:480..1508"
                     /db_xref="GeneID:117611967"
ORIGIN      
        1 mdsptliegn lpdritkrdr erkniierrr eerqslaves eqssyfkdtf yssckkvkem
       61 ldnapsapts alpgmfdkin keilmlknyl sqskmflkvy dirraqenlq vleneasdle
      121 lkllpkkkfg fknrrvvkkt sekthdvtdg lkdlkisegi vngstkqnyk lsskygdsac
      181 mllgkvdeql vldaenvnkn dvllsdlirc tiriygtpst lhmvnlkqct vlvgpvtssv
      241 fahdcnecvf alacqqlrlh sstdctiylh vtsraiiedc tkirvapynw syedqanhfn
      301 lagldpkinn wnciddfnwl snekhspnws ildpelrvks wd
//
LOCUS       XP_034195512             335 aa            linear   INV 03-SEP-2025
DEFINITION  partitioning defective protein 6 [Osmia lignaria lignaria].
ACCESSION   XP_034195512
VERSION     XP_034195512.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339621.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195512.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..335
                     /product="partitioning defective protein 6"
                     /calculated_mol_wt=36526
     Region          15..96
                     /region_name="PB1_Par6"
                     /note="The PB1 domain is an essential part of Par6 protein
                     which in complex with Par3 and aPKC proteins is crucial
                     for establishment of apical-basal polarity of animal
                     cells. The PB1 domain is a modular domain mediating
                     specific protein-protein interactions...; cd06403"
                     /db_xref="CDD:99724"
     Site            order(16,18,25..27,29,44,48,90)
                     /site_type="other"
                     /note="PB1 interaction surface [polypeptide binding]"
                     /db_xref="CDD:99724"
     Region          154..250
                     /region_name="PDZ_Par6-like"
                     /note="PDZ domain of partitioning defective 6 (Par6),
                     Drosophila Rho GTPase-activating protein 100F
                     (RhoGAP100F), and related domains; cd06718"
                     /db_xref="CDD:467202"
     Site            order(166..172,197,200,230,234,237..238)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467202"
     CDS             1..335
                     /gene="par-6"
                     /coded_by="XM_034339621.2:120..1127"
                     /db_xref="GeneID:117611611"
ORIGIN      
        1 msksklhyqv dssivavksk fdadiirfsi nrneaisyed frkllaerhd igpdlnfliw
       61 ytdptdgdll pinndnnlar allaakpllr ifiqrkgdgl edingygtmk pknlissilg
      121 gtpgkpksla isnphdfrqv saiidvdilp etcrrvrllk hgsdkplgfy ikdgesfril
      181 ppsagggiek vpgvfisrlv pgglaestgl lavndevlev ngievagktl dqvtdmmian
      241 ssnliitvkp anqraalaap rrgsfsrnsq lssgshqstq saatgsdeda deivdltgvt
      301 lqddattsts qhhhyhhhhh hhqnhfnhdq gvlhl
//
LOCUS       XP_034196408              98 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase F(0) complex subunit g, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034196408
VERSION     XP_034196408.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340517.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196408.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..98
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..98
                     /product="ATP synthase F(0) complex subunit g,
                     mitochondrial"
                     /calculated_mol_wt=10663
     Region          7..96
                     /region_name="ATP-synt_G"
                     /note="Mitochondrial ATP synthase g subunit; pfam04718"
                     /db_xref="CDD:461408"
     CDS             1..98
                     /gene="LOC117611968"
                     /coded_by="XM_034340517.2:116..412"
                     /db_xref="GeneID:117611968"
ORIGIN      
        1 mskpgiisql lksvavnakp avgrflcyak velvppkisd ipairndikk lvsagkngri
       61 lelsvrevwl ntlvtievlc wffvgecigk chlvgykv
//
LOCUS       XP_034195426            3122 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611571 [Osmia lignaria lignaria].
ACCESSION   XP_034195426
VERSION     XP_034195426.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339535.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195426.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3122
                     /product="uncharacterized protein LOC117611571"
                     /calculated_mol_wt=343088
     Region          12..83
                     /region_name="PWWP"
                     /note="PWWP (Pro-Trp-Trp-Pro) domain; cd05162"
                     /db_xref="CDD:438958"
     Site            order(21,24,53)
                     /site_type="other"
                     /note="putative methyl-histone binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438958"
     Site            22..25
                     /site_type="other"
                     /note="PWWP sequence motif"
                     /db_xref="CDD:438958"
     Region          <230..>1289
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          <272..748
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..3122
                     /gene="LOC117611571"
                     /coded_by="XM_034339535.2:1623..10991"
                     /db_xref="GeneID:117611571"
ORIGIN      
        1 mceqteinyl dgdvvwvklg acwwpgqvtg fhnlpediqv efqkkpliaa vkffqedtye
       61 fvknyqqiyk ynctlkddfi kkgldkyrsk skdgsrymdk fpgdvemaek ltggdpdils
      121 hdkfcpeekp nisalfgdkk ipkrkreree isrrsdsidv vrkithprfl kesdhevrir
      181 qqpsslpstp nssgtpqypc hlcnftssrv nviichiksh rsggdtppsk skvktysqyr
      241 srysdvdtpk rkyprkeksq stksrssses gkrkqskqne kaakkkktdp qirdtiladw
      301 edesdedisl tpdksadlss vhnsstetrf dlddskedsn ekdntvsdtn eliaesekll
      361 eeteklttin vleesmtnss stnfkskldk qlesekddki fdaesnepik nsqkdrdvav
      421 dndnklntsd kedkkallsc fdfheeelsi ppvrkkaraf nqkkeiikef emsqalkteq
      481 ekqiendkie sedcnsnske seksetqass eqekpeesnk avdndankem nndiedtkek
      541 iksekvaedv rksstnaget eldiveifev gkvevdnant andlknvevq dsdvknvske
      601 ltendelkeq nkstdhqqte tkdtitvdtn vesveqkpsk emeeilperf tvetedetvs
      661 epadippeqs ensdadmldq dqvpnsdatn itlssdqsna tlqvrrrrgr prksellran
      721 ssgntsgnes prirekisae mfekfeletr asesdserss sgrkrkpnkk yivsdiyvpd
      781 vdqkvyktde sqsesedkgy knvkvkskrg rkpaeidkkt nentdlmqek eleiscnild
      841 ttgnnvdsas telkddlaen sasknqqfvd vdtkltdtsi pteisekmev sseekvlsek
      901 sdisvlledt ktketedsav ktqgtsleve kitddkmete stitqsdllq enlkdldtes
      961 lgtdqpevpl ekevldpski idmlppkksq ikkfelsqid lfdtdnsdnk isissgpddt
     1021 kekledsnte vieastslee gssliqkgnl sseklekdtk neeivkdtkk veekkkvqtk
     1081 eksvvisqev pqtkvktitk eqesksekfd htfkeltsti seqqiqnktp vhtrykgkcv
     1141 pevgtilkks kgpvpeekst ntfqeaflkt lhmdkrkgsd ddttekgkrg kklskkksee
     1201 ntkieesnta gnlsnlssqd nsqvtlmsnf ggievvvdqn leigtkvddi ilqnvddlip
     1261 ktliqtssge dnsksitldi qkldessrms tstpspssdv tvpvkkremp riienvtlte
     1321 pmqilkskll dklpqkgvkh kletdstkkg dqkgtpktkv mkieslspst kmktsskits
     1381 pnlsltkklq vlkaeeaett tmleaqneql ktlktsptat tsekyiqqss qsladmeldi
     1441 nsmpfvlsed vltpenieqm pvvissiipa sstvsatlsf tpptqmvsta qsqfklnena
     1501 getspskkks gtpailktkn kakptitsik tlvppltggv kgikfqstpa tskvpplltq
     1561 ksapgkyvvv qtsggqqlry svqgksgtqq kiaiptgkst gnaqvvqqgg kvviltsaqs
     1621 gqskmlplsi sktlsgkvqr imtnkgqvfc pintqgiinp sktiiapkgd saspttsklp
     1681 aghkiintqg iitskgvltp isgtiaktal lgtlsqgilt kggvytpist sslggkaiig
     1741 sktlvtksgt ilssqnlgta ktiltpisqa isgrtilsaq givstkgtvl tpitgqqvka
     1801 iaakspskss kiqyqsvqqk mqlpvlqksq kipgsssqgk sgqvksgsst vvlqnavptq
     1861 kavqqgkkim kqtvvqqgsi qgiasqsstq qavgvnpqiq qkgktvsqss iqkviiprgs
     1921 rqtkaqqriv vqkvqeptit tmqnvhskqt vsvpltsvsn iskttiqpkq atnntastkv
     1981 itkgnvinkn tqlqknllna amnsvtttnv ntnlptplpl pplepveser ksklenvlve
     2041 siqkeqskme klnvekgsem ekieepkits qpqimalpte ssdgtqtyvl vtideqgqiq
     2101 pldnntlmsl egttqnpdgt rtlyidpssl geagtldniv lqfdngvlpn iqtsatettq
     2161 aiisetfpts evmqtsnqdi laaalantdf qqeigltent aasvmttglt qtslinqtil
     2221 qstiipptep issasvlets ltlnqpimtp levpsnlpvq sepvptsvas ipsslelpit
     2281 itnpnisyit tgeaqiqipg nsmpdigeii enpstaeisq ieipastqyl vlpnlednia
     2341 ietqtvqsna vsmpsvsysv sipnsielqt sqvqttpsmp iiddtyaenv qftstiessi
     2401 tseqtfvdvs vsdmlaskpa vstesiksqd ttvtsdmmvs hvsseetivs tnepsilsee
     2461 qdttmslqev hsfeevsiks edivskntav kedsgsnvkm dnqteevsnn dntvevemae
     2521 qpddtmtsnl sivgdsslit sesdvkmssv ehasfdvsds neintntlqe sdsikkleql
     2581 sfgeqssskv hspvseeqta tsnevevtqe vveslpaesl nrvdeqsmdv deapeskeca
     2641 assmkesedc qrdepssqge sdlvqrnlel hfeetnaets hetpsqsyeq feiavttdst
     2701 dlpsqsatks ensrepsqsi tyepmetdee sivteppsqs fehskvneas qsyetssaea
     2761 ninaptqsfn elmqsqeest tvdetiddqt fptqsyevgk pdnvpenlet daeisqegnm
     2821 psqsndigtd gigtssfstn ntglnedeta sssyvpetpe nqerdpdqes aistssceip
     2881 pcaelniass saehtsihdn gvpeiptssy nlnpditsth vdsvptssye eqnvstsyev
     2941 pismpgleet psqnfvtest dhrnepssyt hqevtasyyd sveqetnsrl dedpqeevtp
     3001 syydgnphea sqsyftpeea tpsysshnvs psyyghrpet easqsyysas diekteqsss
     3061 qnteasqsfy qeqtdelklt qqgeatptyt erypvdytle sspmerhdlv essvpatrpt
     3121 er
//
LOCUS       XP_034195428             886 aa            linear   INV 03-SEP-2025
DEFINITION  phosphatidylinositol 3-kinase 59F [Osmia lignaria lignaria].
ACCESSION   XP_034195428
VERSION     XP_034195428.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339537.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..886
                     /product="phosphatidylinositol 3-kinase 59F"
                     /calculated_mol_wt=100951
     Region          27..188
                     /region_name="C2_PI3K_class_III"
                     /note="C2 domain present in class III phosphatidylinositol
                     3-kinases (PI3Ks); cd08397"
                     /db_xref="CDD:176042"
     Region          286..495
                     /region_name="PI3Ka_III"
                     /note="Phosphoinositide 3-kinase (PI3K) class III,
                     accessory domain (PIK domain); PIK domain is conserved in
                     all PI3 and PI4-kinases. Its role is unclear but it has
                     been suggested to be involved in substrate presentation.
                     In general, PI3Ks class III...; cd00870"
                     /db_xref="CDD:238442"
     Region          538..882
                     /region_name="PI3Kc_III"
                     /note="Catalytic domain of Class III Phosphoinositide
                     3-kinase; cd00896"
                     /db_xref="CDD:270628"
     Site            order(611,613..615,617,633,635,638,669,681..684,688,691,
                     747,749,759..760)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270628"
     Site            739..747
                     /site_type="active"
                     /note="catalytic loop [active]"
                     /db_xref="CDD:270628"
     Site            760..779
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270628"
     CDS             1..886
                     /gene="Pi3K59F"
                     /coded_by="XM_034339537.2:92..2752"
                     /db_xref="GeneID:117611572"
ORIGIN      
        1 medindkffy vysssldtri qikigtlegk rqrpeydkll idpmlkycgl ygtnggrgdl
       61 aaslqvwagg rplalpvhta ykhftsrwnw nqwvslpisy sdlprdaqlc islydcagpg
      121 rqlpvggtti slfgkhgvfr qgmldlrvwp gveadgdvps stpgktrdhg keqmqrlakl
      181 vkkhrngqmn kidwldrltf reielinere kraseylylm iefpevtmdg ipysivyyek
      241 dgdevvqhrs qpdvvtlpdy eilqenlvea khhklarslr sgghtkelkp tssvrdalnt
      301 ilsyppttal steeqdliwk yrfylsnqkk altkfvkcvn wkvageerqa lemlalwapp
      361 dpedalellg pafthpavrr yaigrlnqap dddlmlyllq lvqalkyedf egikrahqtl
      421 mkekecekve kldndvqind ssstpitast esenghfsts qdclmdlasf litracqntt
      481 lanyfywyls iecedqndpa isakqdtrvk emyitvmsmf stmlaqgnav wqkrraflkr
      541 qkmfidqlva lvkavaresg nrkkktdrlr llladpdpaf kinfsnfepi pfpldpeici
      601 kgiipekasl fksalmpskl tflttdnsey iaifkhgddl rqdqlilqti almdkllrre
      661 nldlkltpyr vlatstrhgf lqfiesttva evlasegsil sffrkhhpse tgpygvvpev
      721 mdtyvrscag yciityvlgv gdrhldnlll ttsgklfhid fgyilgrdpk plpppmklsk
      781 emveamggvg sehyhefrkq cytaflhlrr hanlilnlfs lmvdasvpdi alepdkavkk
      841 vqdklrldls deeavhyvhn lldlsvtavm avlveqlhkf aqywrk
//
LOCUS       XP_034195452             309 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611580 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195452
VERSION     XP_034195452.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339561.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..309
                     /product="uncharacterized protein LOC117611580 isoform X1"
                     /calculated_mol_wt=35276
     CDS             1..309
                     /gene="LOC117611580"
                     /coded_by="XM_034339561.2:217..1146"
                     /db_xref="GeneID:117611580"
ORIGIN      
        1 matavpsrfa vlsiddddck pkktqksvta gktnqksknd kakqqqqqkk ddkkkqnkgk
       61 kkkpnnnnsd nqqweqwkqk dtmaveetfe qelhqaills klsyeeqlvn aaksekepep
      121 nkksgkkskk atmsleefnn mgsnapqeiv lppetgdnks kepdveffer veketkeeit
      181 kgkekdilka rfnkidddit saqlrvevek rdeiikelrt qvdnlkeelt qvkernkkly
      241 qilshgemkd kasvlaevak lqeirdeltt evaslhaqle qersktratn tdakpskqtn
      301 kkrpasena
//
LOCUS       XP_034195453             267 aa            linear   INV 03-SEP-2025
DEFINITION  G kinase-anchoring protein 1-B isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195453
VERSION     XP_034195453.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339562.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..267
                     /product="G kinase-anchoring protein 1-B isoform X2"
                     /calculated_mol_wt=30727
     CDS             1..267
                     /gene="LOC117611580"
                     /coded_by="XM_034339562.2:264..1067"
                     /db_xref="GeneID:117611580"
ORIGIN      
        1 mmtivsrrkl rrvlllgkkk kpnnnnsdnq qweqwkqkdt maveetfeqe lhqaillskl
       61 syeeqlvnaa ksekepepnk ksgkkskkat msleefnnmg snapqeivlp petgdnkske
      121 pdvefferve ketkeeitkg kekdilkarf nkiddditsa qlrvevekrd eiikelrtqv
      181 dnlkeeltqv kernkklyqi lshgemkdka svlaevaklq eirdelttev aslhaqleqe
      241 rsktratntd akpskqtnkk rpasena
//
LOCUS       XP_034196273             826 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196273
VERSION     XP_034196273.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340382.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..826
                     /product="protein groucho isoform X1"
                     /calculated_mol_wt=88874
     Region          68..192
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <537..824
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          541..582
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          590..628
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          633..669
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          676..711
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          716..749
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          757..791
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          798..822
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..826
                     /gene="LOC117611916"
                     /coded_by="XM_034340382.2:71..2551"
                     /db_xref="GeneID:117611916"
ORIGIN      
        1 mkviarfsrt sklvvdslgr sfrisfcpeg slvilkqnis mvlprssmdt nkirqshseg
       61 ppqpgqqykf sigelcdrik edfnflqtqy hslklecekl asekieiqrh yvmyyemsyg
      121 lnvemhkqte iakrlnaiiv qtlpflsqeh qqqvataldr akqvtmteln aiigqqrpdl
      181 prllqqvhaq qlppgaamgp hpgipglpgl gpgaglpvpt sassaallgl gippgaaasa
      241 ggaaghplsi lnkpdlhrgq pddlkhnggl npaeerhrns ispaerekys rprspdpqhh
      301 dhvplkkmkk eqdkdmghqs dgeksdqdlv vddasegpgs paangtsspr engldklgps
      361 svplnsqvkk eapppsprsg tssnastpsa kkmeerekpt tpiskpvtpt saggsssgsg
      421 glkpsssskp lvsasavgpy pphyppphhp pagphvdmlg yppalngypa rpplqqlydp
      481 hgsmraplgp lgvpggkpay sfhvssegqm qpvpfppdal igqgiprhar qintlthgev
      541 vcavtisnpt kyvytggkgc vkvwdinqgg sgsakhvsql dclqrdnyir svkllpdgrt
      601 livggeasnl siwdlasptp rikaeltsna pacyalaisp dskvcfsccs dgniavwdlq
      661 nqslvrqfqg htdgascidi sadgsklwtg gldntvrswd lregrqlqqh dftsqifslg
      721 ycptgewlav gmensnvevl hatkpdkyql hlhescvlsl rfatcgkwfv stgkdnllna
      781 wrtpygasif qskesssvls cdistddkyi vtgsgdkkat vyeviy
//
LOCUS       XP_034196275             811 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196275
VERSION     XP_034196275.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340384.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..811
                     /product="protein groucho isoform X2"
                     /calculated_mol_wt=87057
     Region          68..177
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <522..809
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          526..567
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          575..613
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          618..654
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          661..696
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          701..734
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          742..776
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          783..807
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..811
                     /gene="LOC117611916"
                     /coded_by="XM_034340384.2:68..2503"
                     /db_xref="GeneID:117611916"
ORIGIN      
        1 mkviarfsrt sklvvdslgr sfrisfcpeg slvilkqnis mvlprssmdt nkirqshseg
       61 ppqpgqqykf sigelcdrik edfnflqtqy hsiklelekl aqektemqrh yvmteiakrl
      121 naiivqtlpf lsqehqqqva taldrakqvt mtelnaiigq qrpdlprllq qvhaqqlppg
      181 aamgphpgip glpglgpgag lpvptsassa allglgippg aaasaggaag hplsilnkpd
      241 lhrgqpddlk hngglnpaee rhrnsispae rekysrprsp dpqhhdhvpl kkmkkeqdkd
      301 mghqsdgeks dqdlvvddas egpgspaang tssprengld klgpssvpln sqvkkeappp
      361 sprsgtssna stpsakkmee rekpttpisk pvtptsaggs ssgsgglkps ssskplvsas
      421 avgpypphyp pphhppagph vdmlgyppal ngyparpplq qlydphgsmr aplgplgvpg
      481 gkpaysfhvs segqmqpvpf ppdaligqgi prharqintl thgevvcavt isnptkyvyt
      541 ggkgcvkvwd inqggsgsak hvsqldclqr dnyirsvkll pdgrtlivgg easnlsiwdl
      601 asptprikae ltsnapacya laispdskvc fsccsdgnia vwdlqnqslv rqfqghtdga
      661 scidisadgs klwtggldnt vrswdlregr qlqqhdftsq ifslgycptg ewlavgmens
      721 nvevlhatkp dkyqlhlhes cvlslrfatc gkwfvstgkd nllnawrtpy gasifqskes
      781 ssvlscdist ddkyivtgsg dkkatvyevi y
//
LOCUS       XP_034196276             787 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196276
VERSION     XP_034196276.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340385.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..787
                     /product="protein groucho isoform X3"
                     /calculated_mol_wt=84158
     Region          29..153
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <498..785
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          502..543
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          551..589
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          594..630
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          637..672
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          677..710
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          718..752
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          759..783
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..787
                     /gene="LOC117611916"
                     /coded_by="XM_034340385.2:1457..3820"
                     /db_xref="GeneID:117611916"
ORIGIN      
        1 mypsaginaa avaaaaarhp gppqpgqqyk fsigelcdri kedfnflqtq yhsiklelek
       61 laqektemqr hyvmyyemsy glnvemhkqt eiakrlnaii vqtlpflsqe hqqqvatald
      121 rakqvtmtel naiigqqrpd lprllqqvha qqlppgaamg phpgipglpg lgpgaglpvp
      181 tsassaallg lgippgaaas aggaaghpls ilnkpdlhrg qpddlkhngg lnpaeerhrn
      241 sispaereky srprspdpqh hdhvplkkmk keqdkdmghq sdgeksdqdl vvddasegpg
      301 spaangtssp rengldklgp ssvplnsqvk keapppsprs gtssnastps akkmeerekp
      361 ttpiskpvtp tsaggsssgs gglkpssssk plvsasavgp ypphyppphh ppagphvdml
      421 gyppalngyp arpplqqlyd phgsmraplg plgvpggkpa ysfhvssegq mqpvpfppda
      481 ligqgiprha rqintlthge vvcavtisnp tkyvytggkg cvkvwdinqg gsgsakhvsq
      541 ldclqrdnyi rsvkllpdgr tlivggeasn lsiwdlaspt prikaeltsn apacyalais
      601 pdskvcfscc sdgniavwdl qnqslvrqfq ghtdgascid isadgsklwt ggldntvrsw
      661 dlregrqlqq hdftsqifsl gycptgewla vgmensnvev lhatkpdkyq lhlhescvls
      721 lrfatcgkwf vstgkdnlln awrtpygasi fqskesssvl scdistddky ivtgsgdkka
      781 tvyeviy
//
LOCUS       XP_034196277             787 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034196277
VERSION     XP_034196277.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340386.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..787
                     /product="protein groucho isoform X4"
                     /calculated_mol_wt=84101
     Region          29..153
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <498..785
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          502..543
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          551..589
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          594..630
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          637..672
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          677..710
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          718..752
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          759..783
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..787
                     /gene="LOC117611916"
                     /coded_by="XM_034340386.2:1471..3834"
                     /db_xref="GeneID:117611916"
ORIGIN      
        1 mypsaginaa avaaaaarhp gppqpgqqyk fsigelcdri kedfnflqtq yhslklecek
       61 lasekieiqr hyvmyyemsy glnvemhkqt eiakrlnaii vqtlpflsqe hqqqvatald
      121 rakqvtmtel naiigqqrpd lprllqqvha qqlppgaamg phpgipglpg lgpgaglpvp
      181 tsassaallg lgippgaaas aggaaghpls ilnkpdlhrg qpddlkhngg lnpaeerhrn
      241 sispaereky srprspdpqh hdhvplkkmk keqdkdmghq sdgeksdqdl vvddasegpg
      301 spaangtssp rengldklgp ssvplnsqvk keapppsprs gtssnastps akkmeerekp
      361 ttpiskpvtp tsaggsssgs gglkpssssk plvsasavgp ypphyppphh ppagphvdml
      421 gyppalngyp arpplqqlyd phgsmraplg plgvpggkpa ysfhvssegq mqpvpfppda
      481 ligqgiprha rqintlthge vvcavtisnp tkyvytggkg cvkvwdinqg gsgsakhvsq
      541 ldclqrdnyi rsvkllpdgr tlivggeasn lsiwdlaspt prikaeltsn apacyalais
      601 pdskvcfscc sdgniavwdl qnqslvrqfq ghtdgascid isadgsklwt ggldntvrsw
      661 dlregrqlqq hdftsqifsl gycptgewla vgmensnvev lhatkpdkyq lhlhescvls
      721 lrfatcgkwf vstgkdnlln awrtpygasi fqskesssvl scdistddky ivtgsgdkka
      781 tvyeviy
//
LOCUS       XP_034196278             710 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034196278
VERSION     XP_034196278.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340387.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..710
                     /product="protein groucho isoform X5"
                     /calculated_mol_wt=75356
     Region          <1..76
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <421..708
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          425..466
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          474..512
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          517..553
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          560..595
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          600..633
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          641..675
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          682..706
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..710
                     /gene="LOC117611916"
                     /coded_by="XM_034340387.2:108..2240"
                     /db_xref="GeneID:117611916"
ORIGIN      
        1 msyglnvemh kqteiakrln aiivqtlpfl sqehqqqvat aldrakqvtm telnaiigqq
       61 rpdlprllqq vhaqqlppga amgphpgipg lpglgpgagl pvptsassaa llglgippga
      121 aasaggaagh plsilnkpdl hrgqpddlkh ngglnpaeer hrnsispaer ekysrprspd
      181 pqhhdhvplk kmkkeqdkdm ghqsdgeksd qdlvvddase gpgspaangt ssprengldk
      241 lgpssvplns qvkkeappps prsgtssnas tpsakkmeer ekpttpiskp vtptsaggss
      301 sgsgglkpss sskplvsasa vgpypphypp phhppagphv dmlgyppaln gyparpplqq
      361 lydphgsmra plgplgvpgg kpaysfhvss egqmqpvpfp pdaligqgip rharqintlt
      421 hgevvcavti snptkyvytg gkgcvkvwdi nqggsgsakh vsqldclqrd nyirsvkllp
      481 dgrtlivgge asnlsiwdla sptprikael tsnapacyal aispdskvcf sccsdgniav
      541 wdlqnqslvr qfqghtdgas cidisadgsk lwtggldntv rswdlregrq lqqhdftsqi
      601 fslgycptge wlavgmensn vevlhatkpd kyqlhlhesc vlslrfatcg kwfvstgkdn
      661 llnawrtpyg asifqskess svlscdistd dkyivtgsgd kkatvyeviy
//
LOCUS       XP_034196272             786 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034196272
VERSION     XP_034196272.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340381.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196272.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..786
                     /product="protein groucho isoform X6"
                     /calculated_mol_wt=84464
     Region          28..152
                     /region_name="TLE_N"
                     /note="Groucho/TLE N-terminal Q-rich domain; pfam03920"
                     /db_xref="CDD:461094"
     Region          <497..784
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          501..542
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          550..588
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          593..629
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          636..671
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          676..709
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          717..751
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          758..782
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..786
                     /gene="LOC117611916"
                     /coded_by="XM_034340381.2:117..2477"
                     /db_xref="GeneID:117611916"
ORIGIN      
        1 mvlprssmdt nkirqshseg ppqpgqqykf sigelcdrik edfnflqtqy hsiklelekl
       61 aqektemqrh yvmyyemsyg lnvemhkqte iakrlnaiiv qtlpflsqeh qqqvataldr
      121 akqvtmteln aiigqqrpdl prllqqvhaq qlppgaamgp hpgipglpgl gpgaglpvpt
      181 sassaallgl gippgaaasa ggaaghplsi lnkpdlhrgq pddlkhnggl npaeerhrns
      241 ispaerekys rprspdpqhh dhvplkkmkk eqdkdmghqs dgeksdqdlv vddasegpgs
      301 paangtsspr engldklgps svplnsqvkk eapppsprsg tssnastpsa kkmeerekpt
      361 tpiskpvtpt saggsssgsg glkpsssskp lvsasavgpy pphyppphhp pagphvdmlg
      421 yppalngypa rpplqqlydp hgsmraplgp lgvpggkpay sfhvssegqm qpvpfppdal
      481 igqgiprhar qintlthgev vcavtisnpt kyvytggkgc vkvwdinqgg sgsakhvsql
      541 dclqrdnyir svkllpdgrt livggeasnl siwdlasptp rikaeltsna pacyalaisp
      601 dskvcfsccs dgniavwdlq nqslvrqfqg htdgascidi sadgsklwtg gldntvrswd
      661 lregrqlqqh dftsqifslg ycptgewlav gmensnvevl hatkpdkyql hlhescvlsl
      721 rfatcgkwfv stgkdnllna wrtpygasif qskesssvls cdistddkyi vtgsgdkkat
      781 vyeviy
//
LOCUS       XP_034196279             661 aa            linear   INV 03-SEP-2025
DEFINITION  protein groucho isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034196279
VERSION     XP_034196279.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340388.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="protein groucho isoform X7"
                     /calculated_mol_wt=69795
     Region          <372..659
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          376..417
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          425..463
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          468..504
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          511..546
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          551..584
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          592..626
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          633..657
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..661
                     /gene="LOC117611916"
                     /coded_by="XM_034340388.2:210..2195"
                     /db_xref="GeneID:117611916"
ORIGIN      
        1 mtelnaiigq qrpdlprllq qvhaqqlppg aamgphpgip glpglgpgag lpvptsassa
       61 allglgippg aaasaggaag hplsilnkpd lhrgqpddlk hngglnpaee rhrnsispae
      121 rekysrprsp dpqhhdhvpl kkmkkeqdkd mghqsdgeks dqdlvvddas egpgspaang
      181 tssprengld klgpssvpln sqvkkeappp sprsgtssna stpsakkmee rekpttpisk
      241 pvtptsaggs ssgsgglkps ssskplvsas avgpypphyp pphhppagph vdmlgyppal
      301 ngyparpplq qlydphgsmr aplgplgvpg gkpaysfhvs segqmqpvpf ppdaligqgi
      361 prharqintl thgevvcavt isnptkyvyt ggkgcvkvwd inqggsgsak hvsqldclqr
      421 dnyirsvkll pdgrtlivgg easnlsiwdl asptprikae ltsnapacya laispdskvc
      481 fsccsdgnia vwdlqnqslv rqfqghtdga scidisadgs klwtggldnt vrswdlregr
      541 qlqqhdftsq ifslgycptg ewlavgmens nvevlhatkp dkyqlhlhes cvlslrfatc
      601 gkwfvstgkd nllnawrtpy gasifqskes ssvlscdist ddkyivtgsg dkkatvyevi
      661 y
//
LOCUS       XP_034196319             738 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196319
VERSION     XP_034196319.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340428.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..738
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X1"
                     /calculated_mol_wt=82039
     Region          292..352
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          437..667
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..738
                     /gene="LOC117611927"
                     /coded_by="XM_034340428.2:3258..5474"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mmsrqskdpv qkchsdpgaa stdgtpcspg krisrrivpg iqtssntrkc vltldgysyv
       61 ivasppdrrv trddiavssp daspnaneat sctgtassrl ynpsspgrng qlskqgtlti
      121 vrrssgknks igesfesspp phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk
      181 aalrlrcllr qlqhgeisae llqrnlnyaa rvleavflde tkvnsggvec srssrrgagl
      241 ssasigsgld sdgqqghavv tqkrklrtpv wardaerves aatcrqgvrr rrladeddel
      301 sevqpdavpp evrewlastf trqlattrrk adekpkfrsv ahairagifv driyrrvtnt
      361 almqfpqevv kvlktlddws fdvfslseaa mgapvkylgy dllnrygmih kfkvppavle
      421 cflgrveegy crhrnpyhnn lhaadvaqtm hyilcqtglm nwltdleifa tlvaaiihdy
      481 ehtgttnnfh vmsgsetall yndravlenh hisasfrilk edecnilqnl skeefrefrs
      541 lvidmvlatd msfhfqqlkn mknilslaep svdkskavsl vlhccdishp akrwdlhhrw
      601 tmqlleeffr qgdkektlgl pfsplcdrnn tlvaesqigf iefivepsmq vcsdmletvl
      661 vplsqnvkes vdesnngsaa esrrikigkp wipclaenks iwkeqavrda earaqkeqeq
      721 kasdnredgd tsqaapee
//
LOCUS       XP_034196320             720 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196320
VERSION     XP_034196320.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340429.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..720
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X2"
                     /calculated_mol_wt=79997
     Region          274..334
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          419..649
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..720
                     /gene="LOC117611927"
                     /coded_by="XM_034340429.2:3257..5419"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mmsrqskdpv qkchsdpgaa stdgtpcspg krisrrivpg iqtssntrkc vltldgysyv
       61 ivasppdrrv trddiavssp daspnaneat sctgtassrl ynpsspgrng qlskqgtlti
      121 vrrssgknks igesfesspp phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk
      181 aalrlrcllr qlqhgeisae llqrnlnyaa rvleavflde tkvnsggvec srssrrgagl
      241 ssasigsgld sdgqqghavv tqkrklrtpv warrladedd elsevqpdav ppevrewlas
      301 tftrqlattr rkadekpkfr svahairagi fvdriyrrvt ntalmqfpqe vvkvlktldd
      361 wsfdvfslse aamgapvkyl gydllnrygm ihkfkvppav lecflgrvee gycrhrnpyh
      421 nnlhaadvaq tmhyilcqtg lmnwltdlei fatlvaaiih dyehtgttnn fhvmsgseta
      481 llyndravle nhhisasfri lkedecnilq nlskeefref rslvidmvla tdmsfhfqql
      541 knmknilsla epsvdkskav slvlhccdis hpakrwdlhh rwtmqlleef frqgdkektl
      601 glpfsplcdr nntlvaesqi gfiefiveps mqvcsdmlet vlvplsqnvk esvdesnngs
      661 aaesrrikig kpwipclaen ksiwkeqavr daearaqkeq eqkasdnred gdtsqaapee
//
LOCUS       XP_034196321             718 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196321
VERSION     XP_034196321.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340430.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..718
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X3"
                     /calculated_mol_wt=80049
     Region          272..332
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          417..647
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..718
                     /gene="LOC117611927"
                     /coded_by="XM_034340430.2:3553..5709"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mskqeprgpp ppplpppyph qklfkkcksa tfqldgatyt ivasppdrrv trddiavssp
       61 daspnaneat sctgtassrl ynpsspgrng qlskqgtlti vrrssgknks igesfesspp
      121 phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk aalrlrcllr qlqhgeisae
      181 llqrnlnyaa rvleavflde tkvnsggvec srssrrgagl ssasigsgld sdgqqghavv
      241 tqkrklrtpv wardaerves aatcrqgvrr rrladeddel sevqpdavpp evrewlastf
      301 trqlattrrk adekpkfrsv ahairagifv driyrrvtnt almqfpqevv kvlktlddws
      361 fdvfslseaa mgapvkylgy dllnrygmih kfkvppavle cflgrveegy crhrnpyhnn
      421 lhaadvaqtm hyilcqtglm nwltdleifa tlvaaiihdy ehtgttnnfh vmsgsetall
      481 yndravlenh hisasfrilk edecnilqnl skeefrefrs lvidmvlatd msfhfqqlkn
      541 mknilslaep svdkskavsl vlhccdishp akrwdlhhrw tmqlleeffr qgdkektlgl
      601 pfsplcdrnn tlvaesqigf iefivepsmq vcsdmletvl vplsqnvkes vdesnngsaa
      661 esrrikigkp wipclaenks iwkeqavrda earaqkeqeq kasdnredgd tsqaapee
//
LOCUS       XP_076546817             718 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546817
VERSION     XP_076546817.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690702.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..718
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X3"
                     /calculated_mol_wt=80049
     Region          272..332
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          417..647
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..718
                     /gene="LOC117611927"
                     /coded_by="XM_076690702.1:2209..4365"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mskqeprgpp ppplpppyph qklfkkcksa tfqldgatyt ivasppdrrv trddiavssp
       61 daspnaneat sctgtassrl ynpsspgrng qlskqgtlti vrrssgknks igesfesspp
      121 phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk aalrlrcllr qlqhgeisae
      181 llqrnlnyaa rvleavflde tkvnsggvec srssrrgagl ssasigsgld sdgqqghavv
      241 tqkrklrtpv wardaerves aatcrqgvrr rrladeddel sevqpdavpp evrewlastf
      301 trqlattrrk adekpkfrsv ahairagifv driyrrvtnt almqfpqevv kvlktlddws
      361 fdvfslseaa mgapvkylgy dllnrygmih kfkvppavle cflgrveegy crhrnpyhnn
      421 lhaadvaqtm hyilcqtglm nwltdleifa tlvaaiihdy ehtgttnnfh vmsgsetall
      481 yndravlenh hisasfrilk edecnilqnl skeefrefrs lvidmvlatd msfhfqqlkn
      541 mknilslaep svdkskavsl vlhccdishp akrwdlhhrw tmqlleeffr qgdkektlgl
      601 pfsplcdrnn tlvaesqigf iefivepsmq vcsdmletvl vplsqnvkes vdesnngsaa
      661 esrrikigkp wipclaenks iwkeqavrda earaqkeqeq kasdnredgd tsqaapee
//
LOCUS       XP_034196322             718 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196322
VERSION     XP_034196322.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340431.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..718
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X3"
                     /calculated_mol_wt=80049
     Region          272..332
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          417..647
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..718
                     /gene="LOC117611927"
                     /coded_by="XM_034340431.2:715..2871"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mskqeprgpp ppplpppyph qklfkkcksa tfqldgatyt ivasppdrrv trddiavssp
       61 daspnaneat sctgtassrl ynpsspgrng qlskqgtlti vrrssgknks igesfesspp
      121 phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk aalrlrcllr qlqhgeisae
      181 llqrnlnyaa rvleavflde tkvnsggvec srssrrgagl ssasigsgld sdgqqghavv
      241 tqkrklrtpv wardaerves aatcrqgvrr rrladeddel sevqpdavpp evrewlastf
      301 trqlattrrk adekpkfrsv ahairagifv driyrrvtnt almqfpqevv kvlktlddws
      361 fdvfslseaa mgapvkylgy dllnrygmih kfkvppavle cflgrveegy crhrnpyhnn
      421 lhaadvaqtm hyilcqtglm nwltdleifa tlvaaiihdy ehtgttnnfh vmsgsetall
      481 yndravlenh hisasfrilk edecnilqnl skeefrefrs lvidmvlatd msfhfqqlkn
      541 mknilslaep svdkskavsl vlhccdishp akrwdlhhrw tmqlleeffr qgdkektlgl
      601 pfsplcdrnn tlvaesqigf iefivepsmq vcsdmletvl vplsqnvkes vdesnngsaa
      661 esrrikigkp wipclaenks iwkeqavrda earaqkeqeq kasdnredgd tsqaapee
//
LOCUS       XP_034196324             718 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196324
VERSION     XP_034196324.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340433.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..718
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X3"
                     /calculated_mol_wt=80049
     Region          272..332
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          417..647
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..718
                     /gene="LOC117611927"
                     /coded_by="XM_034340433.2:654..2810"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mskqeprgpp ppplpppyph qklfkkcksa tfqldgatyt ivasppdrrv trddiavssp
       61 daspnaneat sctgtassrl ynpsspgrng qlskqgtlti vrrssgknks igesfesspp
      121 phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk aalrlrcllr qlqhgeisae
      181 llqrnlnyaa rvleavflde tkvnsggvec srssrrgagl ssasigsgld sdgqqghavv
      241 tqkrklrtpv wardaerves aatcrqgvrr rrladeddel sevqpdavpp evrewlastf
      301 trqlattrrk adekpkfrsv ahairagifv driyrrvtnt almqfpqevv kvlktlddws
      361 fdvfslseaa mgapvkylgy dllnrygmih kfkvppavle cflgrveegy crhrnpyhnn
      421 lhaadvaqtm hyilcqtglm nwltdleifa tlvaaiihdy ehtgttnnfh vmsgsetall
      481 yndravlenh hisasfrilk edecnilqnl skeefrefrs lvidmvlatd msfhfqqlkn
      541 mknilslaep svdkskavsl vlhccdishp akrwdlhhrw tmqlleeffr qgdkektlgl
      601 pfsplcdrnn tlvaesqigf iefivepsmq vcsdmletvl vplsqnvkes vdesnngsaa
      661 esrrikigkp wipclaenks iwkeqavrda earaqkeqeq kasdnredgd tsqaapee
//
LOCUS       XP_034196325             687 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196325
VERSION     XP_034196325.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..687
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A isoform X4"
                     /calculated_mol_wt=76789
     Region          241..301
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          386..616
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..687
                     /gene="LOC117611927"
                     /coded_by="XM_034340434.2:3257..5320"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mmsrqskdpv qkchsdpgaa stdgtpcspg krisrrivpg iqtssntrkc vltldgysyv
       61 ivasppdrrv trddiavssp daspnaneat sctgtassrl ynpsspgrng qlskqgtlti
      121 vrrssgknks igesfesspp phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk
      181 aalrlrcllr qlqhgeisae llqrnlnyaa rvleavflde tkdaervesa atcrqgvrrr
      241 rladeddels evqpdavppe vrewlastft rqlattrrka dekpkfrsva hairagifvd
      301 riyrrvtnta lmqfpqevvk vlktlddwsf dvfslseaam gapvkylgyd llnrygmihk
      361 fkvppavlec flgrveegyc rhrnpyhnnl haadvaqtmh yilcqtglmn wltdleifat
      421 lvaaiihdye htgttnnfhv msgsetally ndravlenhh isasfrilke decnilqnls
      481 keefrefrsl vidmvlatdm sfhfqqlknm knilslaeps vdkskavslv lhccdishpa
      541 krwdlhhrwt mqlleeffrq gdkektlglp fsplcdrnnt lvaesqigfi efivepsmqv
      601 csdmletvlv plsqnvkesv desnngsaae srrikigkpw ipclaenksi wkeqavrdae
      661 araqkeqeqk asdnredgdt sqaapee
//
LOCUS       XP_034196326             685 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196326
VERSION     XP_034196326.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340435.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..685
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X5"
                     /calculated_mol_wt=76405
     Region          239..299
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          384..614
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..685
                     /gene="LOC117611927"
                     /coded_by="XM_034340435.2:650..2707"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mdkdrgtqva sppdrrvtrd diavsspdas pnaneatsct gtassrlynp sspgrngqls
       61 kqgtltivrr ssgknksige sfessppphs pdtlsssqsv dldeildnvd lttdslpavd
      121 tpdacdkaal rlrcllrqlq hgeisaellq rnlnyaarvl eavfldetkv nsggvecsrs
      181 srrgaglssa sigsgldsdg qqghavvtqk rklrtpvwar daervesaat crqgvrrrrl
      241 adeddelsev qpdavppevr ewlastftrq lattrrkade kpkfrsvaha iragifvdri
      301 yrrvtntalm qfpqevvkvl ktlddwsfdv fslseaamga pvkylgydll nrygmihkfk
      361 vppavlecfl grveegycrh rnpyhnnlha advaqtmhyi lcqtglmnwl tdleifatlv
      421 aaiihdyeht gttnnfhvms gsetallynd ravlenhhis asfrilkede cnilqnlske
      481 efrefrslvi dmvlatdmsf hfqqlknmkn ilslaepsvd kskavslvlh ccdishpakr
      541 wdlhhrwtmq lleeffrqgd kektlglpfs plcdrnntlv aesqigfief ivepsmqvcs
      601 dmletvlvpl sqnvkesvde snngsaaesr rikigkpwip claenksiwk eqavrdaear
      661 aqkeqeqkas dnredgdtsq aapee
//
LOCUS       XP_034196327             685 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196327
VERSION     XP_034196327.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340436.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..685
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1 isoform X5"
                     /calculated_mol_wt=76405
     Region          239..299
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          384..614
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..685
                     /gene="LOC117611927"
                     /coded_by="XM_034340436.2:716..2773"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mdkdrgtqva sppdrrvtrd diavsspdas pnaneatsct gtassrlynp sspgrngqls
       61 kqgtltivrr ssgknksige sfessppphs pdtlsssqsv dldeildnvd lttdslpavd
      121 tpdacdkaal rlrcllrqlq hgeisaellq rnlnyaarvl eavfldetkv nsggvecsrs
      181 srrgaglssa sigsgldsdg qqghavvtqk rklrtpvwar daervesaat crqgvrrrrl
      241 adeddelsev qpdavppevr ewlastftrq lattrrkade kpkfrsvaha iragifvdri
      301 yrrvtntalm qfpqevvkvl ktlddwsfdv fslseaamga pvkylgydll nrygmihkfk
      361 vppavlecfl grveegycrh rnpyhnnlha advaqtmhyi lcqtglmnwl tdleifatlv
      421 aaiihdyeht gttnnfhvms gsetallynd ravlenhhis asfrilkede cnilqnlske
      481 efrefrslvi dmvlatdmsf hfqqlknmkn ilslaepsvd kskavslvlh ccdishpakr
      541 wdlhhrwtmq lleeffrqgd kektlglpfs plcdrnntlv aesqigfief ivepsmqvcs
      601 dmletvlvpl sqnvkesvde snngsaaesr rikigkpwip claenksiwk eqavrdaear
      661 aqkeqeqkas dnredgdtsq aapee
//
LOCUS       XP_034196328             669 aa            linear   INV 03-SEP-2025
DEFINITION  dual specificity calcium/calmodulin-dependent 3',5'-cyclic
            nucleotide phosphodiesterase 1A isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196328
VERSION     XP_034196328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..669
                     /product="dual specificity calcium/calmodulin-dependent
                     3',5'-cyclic nucleotide phosphodiesterase 1A isoform X6"
                     /calculated_mol_wt=74747
     Region          223..283
                     /region_name="PDEase_I_N"
                     /note="3'5'-cyclic nucleotide phosphodiesterase
                     N-terminal; pfam08499"
                     /db_xref="CDD:462497"
     Region          368..598
                     /region_name="PDEase_I"
                     /note="3'5'-cyclic nucleotide phosphodiesterase;
                     pfam00233"
                     /db_xref="CDD:459723"
     CDS             1..669
                     /gene="LOC117611927"
                     /coded_by="XM_034340437.2:3257..5266"
                     /db_xref="GeneID:117611927"
ORIGIN      
        1 mmsrqskdpv qkchsdpgaa stdgtpcspg krisrrivpg iqtssntrkc vltldgysyv
       61 ivasppdrrv trddiavssp daspnaneat sctgtassrl ynpsspgrng qlskqgtlti
      121 vrrssgknks igesfesspp phspdtlsss qsvdldeild nvdlttdslp avdtpdacdk
      181 aalrlrcllr qlqhgeisae llqrnlnyaa rvleavflde tkrladedde lsevqpdavp
      241 pevrewlast ftrqlattrr kadekpkfrs vahairagif vdriyrrvtn talmqfpqev
      301 vkvlktlddw sfdvfslsea amgapvkylg ydllnrygmi hkfkvppavl ecflgrveeg
      361 ycrhrnpyhn nlhaadvaqt mhyilcqtgl mnwltdleif atlvaaiihd yehtgttnnf
      421 hvmsgsetal lyndravlen hhisasfril kedecnilqn lskeefrefr slvidmvlat
      481 dmsfhfqqlk nmknilslae psvdkskavs lvlhccdish pakrwdlhhr wtmqlleeff
      541 rqgdkektlg lpfsplcdrn ntlvaesqig fiefivepsm qvcsdmletv lvplsqnvke
      601 svdesnngsa aesrrikigk pwipclaenk siwkeqavrd aearaqkeqe qkasdnredg
      661 dtsqaapee
//
LOCUS       XP_034194015             687 aa            linear   INV 03-SEP-2025
DEFINITION  heat shock protein 70 cognate 5 [Osmia lignaria lignaria].
ACCESSION   XP_034194015
VERSION     XP_034194015.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338124.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..687
                     /product="heat shock protein 70 cognate 5"
                     /calculated_mol_wt=74858
     Region          57..664
                     /region_name="dnaK"
                     /note="molecular chaperone DnaK; Provisional; PRK00290"
                     /db_xref="CDD:234715"
     CDS             1..687
                     /gene="Hsc70-5"
                     /coded_by="XM_034338124.2:120..2183"
                     /db_xref="GeneID:117610553"
ORIGIN      
        1 mlaaarlltr sytgitcdvt rrqqfstilk naaapafiap qrptdvhqyr fksegvkgav
       61 vgidlgttfs cvavmegkqa kvienaegsr ttpsyvaftk dgerlvgmpa krqavtnpsn
      121 tfyatkrlig rkfedpevkk dmktvsykiv rasngdawvq ggdgkmysps qigafilmkm
      181 ketaeaylst svknavitvp ayfndsqrqa tkdagqiagl nvlrvinept aaalaygmdk
      241 tedkiiavyd lgggtfdisi leiqkgvfev kstngdtflg gedfdnavvn ylakefkkdq
      301 gldvakdama mqrlkeaaek akielssslq tdinlpyltm dssgpkhlnl klsrskfesl
      361 vgdlikrtvq pcqkalsdae vtksdigevl lvggmtrvpk vqqtvqeifg rqpskavnpd
      421 eavavgaavq ggvlagdvtd vllldvtpls lgietlggvf tklisrntti ptkksqvfst
      481 aadgqtqvei kvhqgerema tdnkllgqft lvgippaprg vpqievtfdi dangivhvsa
      541 rdkgtgkeqq iviqssggls kdeienmvkn aeqyamqdki kkdrveavnq aegiihdtes
      601 kleefkaqlp qdecdklrel vakmretlgk kddlepeeik rqtnelqqas lklfemaykk
      661 maaeregqnq sqsqnqedgp tkkeekn
//
LOCUS       XP_034194022             335 aa            linear   INV 03-SEP-2025
DEFINITION  farnesyl transferase alpha isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194022
VERSION     XP_034194022.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..335
                     /product="farnesyl transferase alpha isoform X1"
                     /calculated_mol_wt=39497
     Region          19..319
                     /region_name="PLN02789"
                     /note="farnesyltranstransferase"
                     /db_xref="CDD:215423"
     CDS             1..335
                     /gene="Fnta"
                     /coded_by="XM_034338131.2:153..1160"
                     /db_xref="GeneID:117610560"
ORIGIN      
        1 mtessdegln ggdgpeessw vlykdrkews dvipvpqddg phpivaiays ekfrdsydyf
       61 railksgeks eralaltedc iwlnpanyta wqyrreilkv lgkdlheelk ygnkmiecns
      121 knyqvwhhrk vivewlqdas keleftenil kkdaknyhaw qhrqwciktf nlfdkeleyv
      181 eqlleedirn nsawnqryfv isnttkfeqs iiekeidfal nkidivkgne sawnylqgyc
      241 cilthdsggl gynekvrrkc eelysagcrt nhllaciidi cqerpmkees adsifhinna
      301 mklckelsek hdkirrrywd fvgqqllekl klesr
//
LOCUS       XP_034194029             332 aa            linear   INV 03-SEP-2025
DEFINITION  farnesyl transferase alpha isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194029
VERSION     XP_034194029.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..332
                     /product="farnesyl transferase alpha isoform X2"
                     /calculated_mol_wt=39127
     Region          19..316
                     /region_name="PLN02789"
                     /note="farnesyltranstransferase"
                     /db_xref="CDD:215423"
     CDS             1..332
                     /gene="Fnta"
                     /coded_by="XM_034338138.2:153..1151"
                     /db_xref="GeneID:117610560"
ORIGIN      
        1 mtessdegln ggdgpeessw vlykdrkews dvipvpqddg phpivaiays ekfrdsydyf
       61 railksgeks eralaltedc iwlnpanyta wqyrreilkv lgkdlheelk ygnkmiecns
      121 knyqvwhhrk vivewlqdas keleftenil kkdaknyhaw qhrqwciktf nlfdkeleyv
      181 eqlleedirn nsawnqryfv isnttkfeqs iiekeidfal nkidivkgne sawnylqgil
      241 thdsgglgyn ekvrrkceel ysagcrtnhl laciidicqe rpmkeesads ifhinnamkl
      301 ckelsekhdk irrrywdfvg qqlleklkle sr
//
LOCUS       XP_034191960            1462 aa            linear   INV 03-SEP-2025
DEFINITION  clathrin interactor lqfR isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191960
VERSION     XP_034191960.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336069.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191960.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1462
                     /product="clathrin interactor lqfR isoform X1"
                     /calculated_mol_wt=166396
     Region          24..149
                     /region_name="ENTH_EpsinR"
                     /note="Epsin N-Terminal Homology (ENTH) domain of
                     Epsin-related protein; cd16989"
                     /db_xref="CDD:340786"
     Site            order(29,33..34,67,74,78)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340786"
     Site            order(52..54,94..96,142..143,146,149)
                     /site_type="other"
                     /note="SNARE interaction site [polypeptide binding]"
                     /db_xref="CDD:340786"
     Region          1103..1217
                     /region_name="Telomere_reg-2"
                     /note="Telomere length regulation protein; pfam10193"
                     /db_xref="CDD:462990"
     CDS             1..1462
                     /gene="lqfR"
                     /coded_by="XM_034336069.2:402..4790"
                     /db_xref="GeneID:117609562"
ORIGIN      
        1 mmnmwkvrel mdkatnvvmn yteteakvre atnddawgpt gammqelaqa tftyeqfpev
       61 msmlwkrmlq enkrnwrrty ksllllnylv rngservvts srehiydlks lenytcidef
      121 gkdqginirh kvrelidfiq dddklreerk kakknkdkyv glsseamgmr fgggdrwtds
      181 pkwgkssida yndwdrdsrg kgfedtnnsd dgeredsdnd vhpspkrggr eyrdtmdnid
      241 rinktaastv stnasparmp raikkvdlga aanygreqsn ngisgsqnns lsikqksknd
      301 ilndifdsqn enntksvvdd dedddddhdd ndddfnpran tqpavqtpsa nadfgdftsa
      361 fgtpsvktkd sndefadfts afnssvtisn hqmqsqssqa qinligtaip ninssitdnv
      421 nnamfmntqs vstppftslt sgntfsqnps mdsnvfdtlq pqmlsnqqtv nnntassntd
      481 llsdldslns lsaagsmdrr tdnansnffm nmnsttpvav nhgaskreks iastenlskh
      541 aanrlleelc ssvgsiknqn sleklksyis dyvkyfpgpl tpqkysnldf dleidpmlhg
      601 kileeviekf dhnwplrent ldpilkqlvi vegstlavfn etlialtsal ketkdrkrmf
      661 tisiilvelm ksdalfsaii dackcrmqnk mqqeefdetw rntiqilisl psrvanklen
      721 ntfdsfslpm yfkaisfyiv raisfisvgs hygitadtks lsflinklvi taksenflpl
      781 ieiltewcfq nknnerntie nilrtldtss iepvavlflk hssihfgvyq ifgnlisdsn
      841 wkytlttkip lmcyydnnnl vinlisylae flnrdhilid llmklldvwg dksalnhtsl
      901 eqhtyitkli imcirkskry lnwnqkidiq allasgmsnh lqcthiilra igmsvgeift
      961 qelfesengq klsfdyngmp tevielvesl kklhvvtsse tekhakvndl vfediefntl
     1021 gdkkmyelgi ecnilpknvv emkrnsknae tdlresitsf eiiskdnvtn esniesnsei
     1081 dsdddlvpyd msydtkisek lrpaylrdlr dnlenektst npdifleslk vseelilsql
     1141 pgddesfaie llrlfivlke icyvenfeil kfkscvaivt iypkecaefl ckqfymdldm
     1201 ysvsqrlffl dvlaesarrl skipvnkdke dtvskakvnp kkkkvsdkvs lfintessqr
     1261 hrvlysddfe dfemmedqit dwseiinkri esntkrytht mkplktfknk fgnvassffy
     1321 pllygfgkqg tclnsgsqif ldqenilliq flktlstimv aaqncllask lgkeilelsw
     1381 tlryhdqakv rmsvienvea vliavpkdvi mnelfetvle irqwlldsfq nvicgdhdke
     1441 crssaaravf lidsiigstf lk
//
LOCUS       XP_034191969            1433 aa            linear   INV 03-SEP-2025
DEFINITION  clathrin interactor lqfR isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191969
VERSION     XP_034191969.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336078.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191969.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1433
                     /product="clathrin interactor lqfR isoform X2"
                     /calculated_mol_wt=163248
     Region          24..149
                     /region_name="ENTH_EpsinR"
                     /note="Epsin N-Terminal Homology (ENTH) domain of
                     Epsin-related protein; cd16989"
                     /db_xref="CDD:340786"
     Site            order(29,33..34,67,74,78)
                     /site_type="other"
                     /note="phosphoinositide binding site [chemical binding]"
                     /db_xref="CDD:340786"
     Site            order(52..54,94..96,142..143,146,149)
                     /site_type="other"
                     /note="SNARE interaction site [polypeptide binding]"
                     /db_xref="CDD:340786"
     Region          1074..1188
                     /region_name="Telomere_reg-2"
                     /note="Telomere length regulation protein; pfam10193"
                     /db_xref="CDD:462990"
     CDS             1..1433
                     /gene="lqfR"
                     /coded_by="XM_034336078.2:403..4704"
                     /db_xref="GeneID:117609562"
ORIGIN      
        1 mmnmwkvrel mdkatnvvmn yteteakvre atnddawgpt gammqelaqa tftyeqfpev
       61 msmlwkrmlq enkrnwrrty ksllllnylv rngservvts srehiydlks lenytcidef
      121 gkdqginirh kvrelidfiq dddklreerk kakknkdkyv glsseamgmr fgggdrwtds
      181 pkwgkssida yndwdrdsrg kgfedtnnsd dgeredsdnd vhpspkrggr eyrdtmdnid
      241 rinktaastv stnasparmp raikkvdlga aanygreqsn nenntksvvd ddedddddhd
      301 dndddfnpra ntqpavqtps anadfgdfts afgtpsvktk dsndefadft safnssvtis
      361 nhqmqsqssq aqinligtai pninssitdn vnnamfmntq svstppftsl tsgntfsqnp
      421 smdsnvfdtl qpqmlsnqqt vnnntassnt dllsdldsln slsaagsmdr rtdnansnff
      481 mnmnsttpva vnhgaskrek siastenlsk haanrlleel cssvgsiknq nsleklksyi
      541 sdyvkyfpgp ltpqkysnld fdleidpmlh gkileeviek fdhnwplren tldpilkqlv
      601 ivegstlavf netlialtsa lketkdrkrm ftisiilvel mksdalfsai idackcrmqn
      661 kmqqeefdet wrntiqilis lpsrvankle nntfdsfslp myfkaisfyi vraisfisvg
      721 shygitadtk slsflinklv itaksenflp lieiltewcf qnknnernti enilrtldts
      781 siepvavlfl khssihfgvy qifgnlisds nwkytlttki plmcyydnnn lvinlisyla
      841 eflnrdhili dllmklldvw gdksalnhts leqhtyitkl iimcirkskr ylnwnqkidi
      901 qallasgmsn hlqcthiilr aigmsvgeif tqelfeseng qklsfdyngm ptevielves
      961 lkklhvvtss etekhakvnd lvfediefnt lgdkkmyelg iecnilpknv vemkrnskna
     1021 etdlresits feiiskdnvt nesniesnse idsdddlvpy dmsydtkise klrpaylrdl
     1081 rdnlenekts tnpdiflesl kvseelilsq lpgddesfai ellrlfivlk eicyvenfei
     1141 lkfkscvaiv tiypkecaef lckqfymdld mysvsqrlff ldvlaesarr lskipvnkdk
     1201 edtvskakvn pkkkkvsdkv slfintessq rhrvlysddf edfemmedqi tdwseiinkr
     1261 iesntkryth tmkplktfkn kfgnvassff ypllygfgkq gtclnsgsqi fldqenilli
     1321 qflktlstim vaaqncllas klgkeilels wtlryhdqak vrmsvienve avliavpkdv
     1381 imnelfetvl eirqwlldsf qnvicgdhdk ecrssaarav flidsiigst flk
//
LOCUS       XP_034194598            1133 aa            linear   INV 03-SEP-2025
DEFINITION  TBC1 domain family member 8/9 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034194598
VERSION     XP_034194598.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338707.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1133
                     /product="TBC1 domain family member 8/9 isoform X1"
                     /calculated_mol_wt=128924
     Region          151..250
                     /region_name="PH-GRAM1_TCB1D9_TCB1D9B"
                     /note="TBC1 domain family members 9 and 9B (TBC1D9 and
                     TBC1D9B) Pleckstrin Homology-Glucosyltransferases,
                     Rab-like GTPase activators and Myotubularins (PH-GRAM)
                     domain, repeat 1; cd13351"
                     /db_xref="CDD:275420"
     Region          301..398
                     /region_name="PH-GRAM2_TCB1D9_TCB1D9B"
                     /note="TBC1 domain family members 9 and 9B (TBC1D9 and
                     TBC1D9B) Pleckstrin Homology-Glucosyltransferases,
                     Rab-like GTPase activators and Myotubularins (PH-GRAM)
                     domain, repeat 2; cd13354"
                     /db_xref="CDD:270161"
     Region          484..692
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..1133
                     /gene="Tbc1d8-9"
                     /coded_by="XM_034338707.2:518..3919"
                     /db_xref="GeneID:117610872"
ORIGIN      
        1 mwvkpqevll analwiteqa tvyfvlqrrk ghgktkgfts iivgtldsvl dtkpppfril
       61 hqtpssevyw eiacslthee ilqdwewlns nlmdtlksfd teeeitefvc ckiqsiiann
      121 vpdsqfadee dpqsfktvsf kfhqlfnipk edklvnyysc sywksrlprq gwlylsvhhm
      181 cfyayilare tkliirwadi telsktnsil fpdsirvvtr dnkehyfsmf lhksetyslm
      241 eqltniamkr lideksgfne drellnklsk nvpkkpsflk rdldarahse ayrlqfrlpg
      301 tekldgcida tlwtpynkry vwgkiylsqn ylcfesrvrg lvslviplre vklteraenq
      361 sfstgidksi lvtterssfl faqihdrdfv vqkisellar sqlsnlggdn afslnsqnds
      421 ssekaksvnw ksqpplmtlf kaplsseaal kqeakekqwe lhfaeygrgv tmyrttetak
      481 liiqgvpqtl rgevwltfsg alnemvmnpg lykslvnqsl gkscqaneei erdlhrslpe
      541 hpafqsdtgi salrrvlsay awknpqigyc qamnivasvl liycseesaf wqlcnvcesl
      601 lpdyydrrvv galvdqglle elaaehlptl harlqelgli kvislswflt iflsvmptss
      661 avnimdcffy dgakvifqia ltvlewnqdk llncrddgea mqlltdylgg vynddgpilp
      721 rpvdsatpnr sisiqtliye aysrygslti ggierlrlkh rlrvvqslee gieknvirsv
      781 ivdkymtmee lqdllslvre elmsqrksep drydptqppy eaykvdfelf rilfgglslw
      841 gkcsqaesla arlfrlmdrn rdgllnfrel vqaigmttta dltqrlklly tlhlpplltp
      901 vdfespthsd gaevaaeatd ffdsmeqsva slempislae eptigtlsrs tslnsqqgdq
      961 swevqsmgsl rsmvaskdsp ldlktvpkms qrhfialwkt lydmfpaqpe eqetyhcias
     1021 igtlllqlgd vgkkfyvdrd esedslllaa tsaqqssmie rspdrngnps siisstdpdw
     1081 sisveqflas altgqaivdf fskrtdltea iatlknrrfn rvhslsdtpv lni
//
LOCUS       XP_034194602            1126 aa            linear   INV 03-SEP-2025
DEFINITION  TBC1 domain family member 8/9 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034194602
VERSION     XP_034194602.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1126
                     /product="TBC1 domain family member 8/9 isoform X2"
                     /calculated_mol_wt=128185
     Region          151..250
                     /region_name="PH-GRAM1_TCB1D9_TCB1D9B"
                     /note="TBC1 domain family members 9 and 9B (TBC1D9 and
                     TBC1D9B) Pleckstrin Homology-Glucosyltransferases,
                     Rab-like GTPase activators and Myotubularins (PH-GRAM)
                     domain, repeat 1; cd13351"
                     /db_xref="CDD:275420"
     Region          301..398
                     /region_name="PH-GRAM2_TCB1D9_TCB1D9B"
                     /note="TBC1 domain family members 9 and 9B (TBC1D9 and
                     TBC1D9B) Pleckstrin Homology-Glucosyltransferases,
                     Rab-like GTPase activators and Myotubularins (PH-GRAM)
                     domain, repeat 2; cd13354"
                     /db_xref="CDD:270161"
     Region          484..692
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     CDS             1..1126
                     /gene="Tbc1d8-9"
                     /coded_by="XM_034338711.2:518..3898"
                     /db_xref="GeneID:117610872"
ORIGIN      
        1 mwvkpqevll analwiteqa tvyfvlqrrk ghgktkgfts iivgtldsvl dtkpppfril
       61 hqtpssevyw eiacslthee ilqdwewlns nlmdtlksfd teeeitefvc ckiqsiiann
      121 vpdsqfadee dpqsfktvsf kfhqlfnipk edklvnyysc sywksrlprq gwlylsvhhm
      181 cfyayilare tkliirwadi telsktnsil fpdsirvvtr dnkehyfsmf lhksetyslm
      241 eqltniamkr lideksgfne drellnklsk nvpkkpsflk rdldarahse ayrlqfrlpg
      301 tekldgcida tlwtpynkry vwgkiylsqn ylcfesrvrg lvslviplre vklteraenq
      361 sfstgidksi lvtterssfl faqihdrdfv vqkisellar sqlsnlggdn afslnsqnds
      421 ssekaksvnw ksqpplmtlf kaplsseaal kqeakekqwe lhfaeygrgv tmyrttetak
      481 liiqgvpqtl rgevwltfsg alnemvmnpg lykslvnqsl gkscqaneei erdlhrslpe
      541 hpafqsdtgi salrrvlsay awknpqigyc qamnivasvl liycseesaf wqlcnvcesl
      601 lpdyydrrvv galvdqglle elaaehlptl harlqelgli kvislswflt iflsvmptss
      661 avnimdcffy dgakvifqia ltvlewnqdk llncrddgea mqlltdylgg vynddgpilp
      721 rpvdsatpnr sisiqtliye aysrygslti ggierlrlkh rlrvvqslee gieknvirsv
      781 ivdkymtmee lqdllslvre elmsqrksep drydptqppy eaykvdfelf rilfgglslw
      841 gkcsqaesla arlfrlmdrn rdgllnfrel vqaigmttta dltqrlklly tlhlpplltp
      901 vdfespthsd gaevaaeatd ffdsmeqsva slempislae eptigtlsrs tslnsqqgdq
      961 swevqsmgsl rsmvaskdsp ldlktvpkms qrhfialwkt lydmfpaqpe eqetyhcias
     1021 igdvgkkfyv drdesedsll laatsaqqss mierspdrng npssiisstd pdwsisveqf
     1081 lasaltgqai vdffskrtdl teaiatlknr rfnrvhslsd tpvlni
//
LOCUS       XP_076546818             921 aa            linear   INV 03-SEP-2025
DEFINITION  TBC1 domain family member 8/9 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546818
VERSION     XP_076546818.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690703.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..921
                     /product="TBC1 domain family member 8/9 isoform X3"
                     /calculated_mol_wt=105697
     Region          151..250
                     /region_name="PH-GRAM1_TCB1D9_TCB1D9B"
                     /note="TBC1 domain family members 9 and 9B (TBC1D9 and
                     TBC1D9B) Pleckstrin Homology-Glucosyltransferases,
                     Rab-like GTPase activators and Myotubularins (PH-GRAM)
                     domain, repeat 1; cd13351"
                     /db_xref="CDD:275420"
     Region          301..398
                     /region_name="PH-GRAM2_TCB1D9_TCB1D9B"
                     /note="TBC1 domain family members 9 and 9B (TBC1D9 and
                     TBC1D9B) Pleckstrin Homology-Glucosyltransferases,
                     Rab-like GTPase activators and Myotubularins (PH-GRAM)
                     domain, repeat 2; cd13354"
                     /db_xref="CDD:270161"
     Region          484..692
                     /region_name="TBC"
                     /note="Domain in Tre-2, BUB2p, and Cdc16p. Probable
                     Rab-GAPs; smart00164"
                     /db_xref="CDD:214540"
     Region          <824..874
                     /region_name="FRQ1"
                     /note="Ca2+-binding protein, EF-hand superfamily [Signal
                     transduction mechanisms]; COG5126"
                     /db_xref="CDD:444056"
     CDS             1..921
                     /gene="Tbc1d8-9"
                     /coded_by="XM_076690703.1:518..3283"
                     /db_xref="GeneID:117610872"
ORIGIN      
        1 mwvkpqevll analwiteqa tvyfvlqrrk ghgktkgfts iivgtldsvl dtkpppfril
       61 hqtpssevyw eiacslthee ilqdwewlns nlmdtlksfd teeeitefvc ckiqsiiann
      121 vpdsqfadee dpqsfktvsf kfhqlfnipk edklvnyysc sywksrlprq gwlylsvhhm
      181 cfyayilare tkliirwadi telsktnsil fpdsirvvtr dnkehyfsmf lhksetyslm
      241 eqltniamkr lideksgfne drellnklsk nvpkkpsflk rdldarahse ayrlqfrlpg
      301 tekldgcida tlwtpynkry vwgkiylsqn ylcfesrvrg lvslviplre vklteraenq
      361 sfstgidksi lvtterssfl faqihdrdfv vqkisellar sqlsnlggdn afslnsqnds
      421 ssekaksvnw ksqpplmtlf kaplsseaal kqeakekqwe lhfaeygrgv tmyrttetak
      481 liiqgvpqtl rgevwltfsg alnemvmnpg lykslvnqsl gkscqaneei erdlhrslpe
      541 hpafqsdtgi salrrvlsay awknpqigyc qamnivasvl liycseesaf wqlcnvcesl
      601 lpdyydrrvv galvdqglle elaaehlptl harlqelgli kvislswflt iflsvmptss
      661 avnimdcffy dgakvifqia ltvlewnqdk llncrddgea mqlltdylgg vynddgpilp
      721 rpvdsatpnr sisiqtliye aysrygslti ggierlrlkh rlrvvqslee gieknvirsv
      781 ivdkymtmee lqdllslvre elmsqrksep drydptqppy eaykvdfelf rilfgglslw
      841 gkcsqaesla arlfrlmdrn rdgllnfrel vqaigmttta dltqrlklly tlhlpplltp
      901 vdfespthsv yilreplflv a
//
LOCUS       XP_034191981             496 aa            linear   INV 03-SEP-2025
DEFINITION  nop56 ribonucleoprotein [Osmia lignaria lignaria].
ACCESSION   XP_034191981
VERSION     XP_034191981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="nop56 ribonucleoprotein"
                     /calculated_mol_wt=54982
     Region          5..70
                     /region_name="NOP5NT"
                     /note="NOP5NT (NUC127) domain; pfam08156"
                     /db_xref="CDD:462382"
     Region          177..408
                     /region_name="Nop"
                     /note="snoRNA binding domain, fibrillarin; pfam01798"
                     /db_xref="CDD:460335"
     CDS             1..496
                     /gene="Nop56"
                     /coded_by="XM_034336090.2:234..1724"
                     /db_xref="GeneID:117609572"
ORIGIN      
        1 msklyilfeh aagyalfyvk efeevgmllp qveasvtdlq rfhsvvklig fspfktalaa
       61 lesinniseg vvpedlqlfl dscvpkagkn dkivlgvadp klgasiteal gikcdhtgvv
      121 peiirgirfh fhnlvkgfts kssgiaqlgl ghsysrakvk fnvnrvdnmi iqsialldql
      181 dkdvntfsmr irewysyhfp elvkivpeny myakvaqaik nrkeltdekl ealeeivmds
      241 akaqaiidas kssmgmdisp vdllniemfa arvialadyr kqlaeylrsk magvapnlat
      301 ligdqvgarl iahagsltnl akypastvqi lgaekalfra lktrgntpky gllfhstfig
      361 ragaknkgri srylankcsi asridcftdi ptsvfgeklr qqvedrlkfy etgeipkkni
      421 dvmkealqea ahiiasmeqe spkkkkkkkd kkrksegevl ehgkengfie ngvleqseep
      481 vkkkkkkksk slgsde
//
LOCUS       XP_034191995             333 aa            linear   INV 03-SEP-2025
DEFINITION  mitotic checkpoint protein Bub3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191995
VERSION     XP_034191995.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336104.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="mitotic checkpoint protein Bub3 isoform X1"
                     /calculated_mol_wt=37223
     Region          21..301
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          24..61
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(39,43,49..50,61..62,79,83,89..90,101..102,119,124,
                     130..131,143,160,169..170,184..185,203,207,215..216,
                     239..240,257,262,268..269,281..282,300)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          67..101
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          106..142
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          150..182
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          189..229
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          244..290
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          297..322
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..333
                     /gene="Bub3"
                     /coded_by="XM_034336104.2:16..1017"
                     /db_xref="GeneID:117609577"
ORIGIN      
        1 mdsgelrmes rtefkikspp tdaisavefg pnstqfllvs swdstvrlyd ihantmrlky
       61 nhdlpvldva fqdavhaysg glgntlkmyd insntesvmg thdkpirkie ycaavnailt
      121 ggwdaavklw dprtptcvgs ylqpdvvlal svcgdkfvvg takrkvciwd lrnmagmfqr
      181 resslkyqtr cikgfpneqg yvlssiegrv aveyldttpe aqkkkyafkc hrikennveh
      241 iypvnaisfh styntfatgg sdgyvniwdg fnkkrlcqfh rynagvaals fshdgsvlai
      301 gvsylneaei ppggnderei yiryvndqet kpk
//
LOCUS       XP_034192005             326 aa            linear   INV 03-SEP-2025
DEFINITION  mitotic checkpoint protein Bub3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192005
VERSION     XP_034192005.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336114.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="mitotic checkpoint protein Bub3 isoform X2"
                     /calculated_mol_wt=36434
     Region          14..294
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          17..54
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(32,36,42..43,54..55,72,76,82..83,94..95,112,117,
                     123..124,136,153,162..163,177..178,196,200,208..209,
                     232..233,250,255,261..262,274..275,293)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          60..94
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          99..135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          143..175
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          182..222
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          237..283
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          290..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..326
                     /gene="Bub3"
                     /coded_by="XM_034336114.2:141..1121"
                     /db_xref="GeneID:117609577"
ORIGIN      
        1 mesrtefkik spptdaisav efgpnstqfl lvsswdstvr lydihantmr lkynhdlpvl
       61 dvafqdavha ysgglgntlk mydinsntes vmgthdkpir kieycaavna iltggwdaav
      121 klwdprtptc vgsylqpdvv lalsvcgdkf vvgtakrkvc iwdlrnmagm fqrresslky
      181 qtrcikgfpn eqgyvlssie grvaveyldt tpeaqkkkya fkchrikenn vehiypvnai
      241 sfhstyntfa tggsdgyvni wdgfnkkrlc qfhrynagva alsfshdgsv laigvsylne
      301 aeippggnde reiyiryvnd qetkpk
//
LOCUS       XP_034192024             326 aa            linear   INV 03-SEP-2025
DEFINITION  mitotic checkpoint protein Bub3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192024
VERSION     XP_034192024.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336133.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="mitotic checkpoint protein Bub3 isoform X2"
                     /calculated_mol_wt=36434
     Region          14..294
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          17..54
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(32,36,42..43,54..55,72,76,82..83,94..95,112,117,
                     123..124,136,153,162..163,177..178,196,200,208..209,
                     232..233,250,255,261..262,274..275,293)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          60..94
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          99..135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          143..175
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          182..222
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          237..283
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          290..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..326
                     /gene="Bub3"
                     /coded_by="XM_034336133.2:210..1190"
                     /db_xref="GeneID:117609577"
ORIGIN      
        1 mesrtefkik spptdaisav efgpnstqfl lvsswdstvr lydihantmr lkynhdlpvl
       61 dvafqdavha ysgglgntlk mydinsntes vmgthdkpir kieycaavna iltggwdaav
      121 klwdprtptc vgsylqpdvv lalsvcgdkf vvgtakrkvc iwdlrnmagm fqrresslky
      181 qtrcikgfpn eqgyvlssie grvaveyldt tpeaqkkkya fkchrikenn vehiypvnai
      241 sfhstyntfa tggsdgyvni wdgfnkkrlc qfhrynagva alsfshdgsv laigvsylne
      301 aeippggnde reiyiryvnd qetkpk
//
LOCUS       XP_034192015             326 aa            linear   INV 03-SEP-2025
DEFINITION  mitotic checkpoint protein Bub3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192015
VERSION     XP_034192015.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336124.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="mitotic checkpoint protein Bub3 isoform X2"
                     /calculated_mol_wt=36434
     Region          14..294
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          17..54
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(32,36,42..43,54..55,72,76,82..83,94..95,112,117,
                     123..124,136,153,162..163,177..178,196,200,208..209,
                     232..233,250,255,261..262,274..275,293)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          60..94
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          99..135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          143..175
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          182..222
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          237..283
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          290..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..326
                     /gene="Bub3"
                     /coded_by="XM_034336124.2:79..1059"
                     /db_xref="GeneID:117609577"
ORIGIN      
        1 mesrtefkik spptdaisav efgpnstqfl lvsswdstvr lydihantmr lkynhdlpvl
       61 dvafqdavha ysgglgntlk mydinsntes vmgthdkpir kieycaavna iltggwdaav
      121 klwdprtptc vgsylqpdvv lalsvcgdkf vvgtakrkvc iwdlrnmagm fqrresslky
      181 qtrcikgfpn eqgyvlssie grvaveyldt tpeaqkkkya fkchrikenn vehiypvnai
      241 sfhstyntfa tggsdgyvni wdgfnkkrlc qfhrynagva alsfshdgsv laigvsylne
      301 aeippggnde reiyiryvnd qetkpk
//
LOCUS       XP_034192032             326 aa            linear   INV 03-SEP-2025
DEFINITION  mitotic checkpoint protein Bub3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192032
VERSION     XP_034192032.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336141.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="mitotic checkpoint protein Bub3 isoform X2"
                     /calculated_mol_wt=36434
     Region          14..294
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          17..54
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(32,36,42..43,54..55,72,76,82..83,94..95,112,117,
                     123..124,136,153,162..163,177..178,196,200,208..209,
                     232..233,250,255,261..262,274..275,293)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          60..94
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          99..135
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          143..175
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          182..222
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          237..283
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          290..315
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..326
                     /gene="Bub3"
                     /coded_by="XM_034336141.2:54..1034"
                     /db_xref="GeneID:117609577"
ORIGIN      
        1 mesrtefkik spptdaisav efgpnstqfl lvsswdstvr lydihantmr lkynhdlpvl
       61 dvafqdavha ysgglgntlk mydinsntes vmgthdkpir kieycaavna iltggwdaav
      121 klwdprtptc vgsylqpdvv lalsvcgdkf vvgtakrkvc iwdlrnmagm fqrresslky
      181 qtrcikgfpn eqgyvlssie grvaveyldt tpeaqkkkya fkchrikenn vehiypvnai
      241 sfhstyntfa tggsdgyvni wdgfnkkrlc qfhrynagva alsfshdgsv laigvsylne
      301 aeippggnde reiyiryvnd qetkpk
//
LOCUS       XP_034194613             330 aa            linear   INV 03-SEP-2025
DEFINITION  dephosphocoenzyme A carrier [Osmia lignaria lignaria].
ACCESSION   XP_034194613
VERSION     XP_034194613.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338722.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="dephosphocoenzyme A carrier"
                     /calculated_mol_wt=36634
     Region          45..300
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..330
                     /gene="DPCoAC"
                     /coded_by="XM_034338722.2:438..1430"
                     /db_xref="GeneID:117610881"
ORIGIN      
        1 msvvvdtike aslsmemnlq dkdvrkkeek higtdgisnt qrvwtslvsg aiagalaktt
       61 iapldrtkin fqisnqkysa raainflikt lktegllslw rgnsatmvri ipysavqfta
      121 heqwkrilgi ngsergkpgl nflagslagi tsqgttypld lmrarmavtq kaeyrtlrqi
      181 fvriymeegi layyrgfpat llgvipyagc sfftydllrn lltvytvaip dfstslicgg
      241 iagmiaqtss ypldivrrrm qtsaikgqdy htikstvmki ykeegimafy kglsmnwvkg
      301 piavgisfat hdtirdalrk fivrqnnsqn
//
LOCUS       XP_034194620             330 aa            linear   INV 03-SEP-2025
DEFINITION  dephosphocoenzyme A carrier [Osmia lignaria lignaria].
ACCESSION   XP_034194620
VERSION     XP_034194620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="dephosphocoenzyme A carrier"
                     /calculated_mol_wt=36634
     Region          45..300
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..330
                     /gene="DPCoAC"
                     /coded_by="XM_034338729.2:144..1136"
                     /db_xref="GeneID:117610881"
ORIGIN      
        1 msvvvdtike aslsmemnlq dkdvrkkeek higtdgisnt qrvwtslvsg aiagalaktt
       61 iapldrtkin fqisnqkysa raainflikt lktegllslw rgnsatmvri ipysavqfta
      121 heqwkrilgi ngsergkpgl nflagslagi tsqgttypld lmrarmavtq kaeyrtlrqi
      181 fvriymeegi layyrgfpat llgvipyagc sfftydllrn lltvytvaip dfstslicgg
      241 iagmiaqtss ypldivrrrm qtsaikgqdy htikstvmki ykeegimafy kglsmnwvkg
      301 piavgisfat hdtirdalrk fivrqnnsqn
//
LOCUS       XP_034194629             330 aa            linear   INV 03-SEP-2025
DEFINITION  dephosphocoenzyme A carrier [Osmia lignaria lignaria].
ACCESSION   XP_034194629
VERSION     XP_034194629.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338738.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..330
                     /product="dephosphocoenzyme A carrier"
                     /calculated_mol_wt=36634
     Region          45..300
                     /region_name="PTZ00169"
                     /note="ADP/ATP transporter on adenylate translocase;
                     Provisional"
                     /db_xref="CDD:240302"
     CDS             1..330
                     /gene="DPCoAC"
                     /coded_by="XM_034338738.2:219..1211"
                     /db_xref="GeneID:117610881"
ORIGIN      
        1 msvvvdtike aslsmemnlq dkdvrkkeek higtdgisnt qrvwtslvsg aiagalaktt
       61 iapldrtkin fqisnqkysa raainflikt lktegllslw rgnsatmvri ipysavqfta
      121 heqwkrilgi ngsergkpgl nflagslagi tsqgttypld lmrarmavtq kaeyrtlrqi
      181 fvriymeegi layyrgfpat llgvipyagc sfftydllrn lltvytvaip dfstslicgg
      241 iagmiaqtss ypldivrrrm qtsaikgqdy htikstvmki ykeegimafy kglsmnwvkg
      301 piavgisfat hdtirdalrk fivrqnnsqn
//
LOCUS       XP_034194638              83 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S21 [Osmia lignaria lignaria].
ACCESSION   XP_034194638
VERSION     XP_034194638.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338747.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..83
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..83
                     /product="ribosomal protein S21"
                     /calculated_mol_wt=9092
     Region          1..80
                     /region_name="Ribosomal_S21e"
                     /note="Ribosomal protein S21e; pfam01249"
                     /db_xref="CDD:460133"
     CDS             1..83
                     /gene="RpS21"
                     /coded_by="XM_034338747.2:134..385"
                     /db_xref="GeneID:117610896"
ORIGIN      
        1 mendngvlvd lyiprkcsss nriihakdha siqlsiadvd petgrmtdsq kmyaicgair
       61 rmgesddclv rlakndgilp knf
//
LOCUS       XP_034196256            2942 aa            linear   INV 03-SEP-2025
DEFINITION  protein tudor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196256
VERSION     XP_034196256.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340365.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196256.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2942
                     /product="protein tudor isoform X1"
                     /calculated_mol_wt=332568
     Region          <77..166
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          305..419
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          501..614
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          934..1045
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1153..1265
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1367..1479
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1544..1656
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1728..1843
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1925..2031
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          2107..2217
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <2287..2413
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     Region          2356..2865
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..2942
                     /gene="LOC117611909"
                     /coded_by="XM_034340365.2:94..8922"
                     /db_xref="GeneID:117611909"
ORIGIN      
        1 mkakscrknh rfiyvitqkl vhhirghkfy namniqknle eviifvthve aegpflkiwg
       61 qidknsatcv ermilplveq ftrsqgcigp laanlhinal ccarfqnegy yrarviniqp
      121 dgmvvlqfid ygnievlppq eihlldtipg seslksfppv afdfalmnvl pindvwenki
      181 iesikkslwy neykiligsv vnnyrfiklw ynnedfsell ikkhmaigat lhdmfrpkci
      241 qqpppsncpt mtpidnmnll rsdgnemhry qpnvplccat lqkqmmqapv qealvfksrv
      301 ldvpskhdvy vsfvedgphk fsvhlistse ilrvlmrein ahpleplqep plpgsvclgr
      361 ftqdkvlcra vvmsvtdskc klyyvdfght ellpytdifq lpphfinprv lsirftlsgv
      421 yelnatdemk qyfkdivsgk plvlhvrppe gpplvqygdl ydneknikdi lrqafptpgt
      481 mtpaaaltyq epkkllkgte edvlvsfves ckkffvqlqn nvkslelvmn hlaefcktap
      541 ilslshlnig lpcaalydnq wyraqiigin qnsikvlyvd ygneetvpls slrfiredlv
      601 kklsaqaikc vlngwgllpc tqelydqfel milekslrlr vvdvtangvv vdlyepetme
      661 diksqmlqpt edekkvinqp nyetmeeqqp vkispkmnqs estnkwhkkd qsdswrqppn
      721 rdfaqeknkf ktwkdesneg klndarndkg nfhrndtrne rfnrddfsnn rntrdkwnnk
      781 neyndsfkgg rsprgggrnk tsgyggfknh sssdkswsdk dsdtsskgss rrgrngstrg
      841 yskregfsgr iqrdkysdke ndrsfrggkt nsdfsrgndr frdkkgqgyr lsqnklnavn
      901 sfsdgeprry spmrnrnevr ipspnidigs tktcevvftt slsdffiqls pdytaldsvm
      961 eniasiyeeg gellkeseil sgvyciaqys vdlkwyrali ksvegnsatv qfvdygniet
     1021 vefdkikviq eeflklpaqa vhckllglkd daldnsrvkh fedkvtektl eaefiieeng
     1081 rycvllrevi egwptntfin eefcknidlq kakedalfsr ktspttkqfn lpdyassdak
     1141 wttisdilgt tkdaiitwft npnnfycqvl dnetefrtmm neiqkiyvgr epvpsytlqi
     1201 gssviaifse dgalyraeil elnklnghlv qyidfgnsav vntnkiypve kklmhlpkqa
     1261 vhcsllnivp kdnqgwlkin taaidncfna drfecvfhdl kddkylisls nngndvaatl
     1321 vekdlatfas esksdaaveg sdtttsavsp nvervdinll sgqvlrvkvs svenaskfyv
     1381 qlpsasecet rinsymankd pevmqrlaah eiclgtgcli ysngvwrrav innhsrtagf
     1441 dvkfidtgac deilsdsvla lpgelsvmqn qaiecsladv vsssetdnal kkhaegkevi
     1501 icvnevdnnr livkvfdlfg nkikvsedsd ekispicpmp ilssthkvsv syadhsasiw
     1561 lqrnaeyild tnlsealdqh ystsgkliep tvglvcaaks vdghwyrakv ikctengvyv
     1621 nfidygnnee vtsdvvtale pcfyiphqla invslpvtln cpipeqlsvl qnylsnkelt
     1681 avfrntnnkw vvellddgek isdkfrslna eelsstgkpy ihnmtvgsty nvyvshtdsp
     1741 sqfwlqhvdd atelstkqee lqeqaptfpa vegileegsl cvavytidnl wyraqvldad
     1801 edittvrfid ygntdiidnk svnirqipds wkeikeysvk crldvipvds edwnvttcek
     1861 fenlvtsves lqasiiansi pkrvdlling ksisetlvee hqavkihree elideivdle
     1921 ldphsafvsh vnspsefwvq eeksvgdlev mtdrfivahm fpkveeiken llcvakyped
     1981 gywyrarvvs hsdnatrviy idygnsatst eicaipedla iipplsrkcs lvmpegitew
     2041 sqraceeftk laadgatifl ldvlkedets lvkltlngen vtdilanfce rfppiieerl
     2101 pplgeenspn vfvtrvnspd dfwiqtesst adlntmtdrl qaapsflpln tfetgticaa
     2161 kysedeqwyr akilshseng tevlfidygn vevtnetrvl pvdiinippl akhcamqkpn
     2221 sisewsqemr ktfeelaadg ettfqfeild dtndpmhvkl slegtnvvdi ittytedess
     2281 dvaiakedsv kdeninktad eethepvend eareqeteen vnnecsnmns sveltideiv
     2341 qsmkpdelee eetiesnnin letnenddvk kvttdleeqi eiqssidvnq tqkteidtea
     2401 skndletqnv nekevalnvi evtkldsvst lvstttdlqn peeeqtnisg skiesidnvr
     2461 dttelespnk esvttdeiee icelqkdvqs snlneentve vestseqsav ttdkieeice
     2521 lkkdvqssnl neentveles pnkesvttdk ieeicelkkd kqdsnlneen iievestneq
     2581 yvttdkkdei relkkdvess nlneentlev estseqsltt dkkeeicelk kdvqssnlnk
     2641 estaelespk kesvttdkke eicelnkgiq nnneentakl estseqhvtn dnkeetcelk
     2701 tntqsnneen telkaaneqs vtndkkeeic elkkdiqssn fneesiaele spkkesvttd
     2761 kkeeiceskk dersnnlnee ssaklestse hhvtndnkee tcglktdiqs nneenselkt
     2821 aneqsvtsdt ctevsqievv ngdeinneek ksdsencklq denkdnvstt gpkdcseika
     2881 dkvdkkecld vqfveianne cskiidtpkt islsdlsvst nvekkkdnen slpsegtsds
     2941 ck
//
LOCUS       XP_034196257            2910 aa            linear   INV 03-SEP-2025
DEFINITION  protein tudor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196257
VERSION     XP_034196257.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196257.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2910
                     /product="protein tudor isoform X2"
                     /calculated_mol_wt=328660
     Region          <45..134
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          273..387
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          469..582
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          902..1013
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1121..1233
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1335..1447
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1512..1624
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1696..1811
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1893..1999
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          2075..2185
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <2255..2380
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     Region          2324..2833
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..2910
                     /gene="LOC117611909"
                     /coded_by="XM_034340366.2:317..9049"
                     /db_xref="GeneID:117611909"
ORIGIN      
        1 mniqknleev iifvthveae gpflkiwgqi dknsatcver milplveqft rsqgcigpla
       61 anlhinalcc arfqnegyyr arviniqpdg mvvlqfidyg nievlppqei hlldtipgse
      121 slksfppvaf dfalmnvlpi ndvwenkiie sikkslwyne ykiligsvvn nyrfiklwyn
      181 nedfsellik khmaigatlh dmfrpkciqq pppsncptmt pidnmnllrs dgnemhryqp
      241 nvplccatlq kqmmqapvqe alvfksrvld vpskhdvyvs fvedgphkfs vhlistseil
      301 rvlmreinah pleplqeppl pgsvclgrft qdkvlcravv msvtdskckl yyvdfghtel
      361 lpytdifqlp phfinprvls irftlsgvye lnatdemkqy fkdivsgkpl vlhvrppegp
      421 plvqygdlyd neknikdilr qafptpgtmt paaaltyqep kkllkgteed vlvsfvesck
      481 kffvqlqnnv kslelvmnhl aefcktapil slshlniglp caalydnqwy raqiiginqn
      541 sikvlyvdyg neetvplssl rfiredlvkk lsaqaikcvl ngwgllpctq elydqfelmi
      601 lekslrlrvv dvtangvvvd lyepetmedi ksqmlqpted ekkvinqpny etmeeqqpvk
      661 ispkmnqses tnkwhkkdqs dswrqppnrd faqeknkfkt wkdesnegkl ndarndkgnf
      721 hrndtrnerf nrddfsnnrn trdkwnnkne yndsfkggrs prgggrnkts gyggfknhss
      781 sdkswsdkds dtsskgssrr grngstrgys kregfsgriq rdkysdkend rsfrggktns
      841 dfsrgndrfr dkkgqgyrls qnklnavnsf sdgeprrysp mrnrnevrip spnidigstk
      901 tcevvfttsl sdffiqlspd ytaldsvmen iasiyeegge llkeseilsg vyciaqysvd
      961 lkwyraliks vegnsatvqf vdygnietve fdkikviqee flklpaqavh ckllglkdda
     1021 ldnsrvkhfe dkvtektlea efiieengry cvllrevieg wptntfinee fcknidlqka
     1081 kedalfsrkt spttkqfnlp dyassdakwt tisdilgttk daiitwftnp nnfycqvldn
     1141 etefrtmmne iqkiyvgrep vpsytlqigs sviaifsedg alyraeilel nklnghlvqy
     1201 idfgnsavvn tnkiypvekk lmhlpkqavh csllnivpkd nqgwlkinta aidncfnadr
     1261 fecvfhdlkd dkylislsnn gndvaatlve kdlatfases ksdaavegsd tttsavspnv
     1321 ervdinllsg qvlrvkvssv enaskfyvql psasecetri nsymankdpe vmqrlaahei
     1381 clgtgcliys ngvwrravin nhsrtagfdv kfidtgacde ilsdsvlalp gelsvmqnqa
     1441 iecsladvvs ssetdnalkk haegkeviic vnevdnnrli vkvfdlfgnk ikvsedsdek
     1501 ispicpmpil ssthkvsvsy adhsasiwlq rnaeyildtn lsealdqhys tsgklieptv
     1561 glvcaaksvd ghwyrakvik ctengvyvnf idygnneevt sdvvtalepc fyiphqlain
     1621 vslpvtlncp ipeqlsvlqn ylsnkeltav frntnnkwvv ellddgekis dkfrslnaee
     1681 lsstgkpyih nmtvgstynv yvshtdspsq fwlqhvddat elstkqeelq eqaptfpave
     1741 gileegslcv avytidnlwy raqvldaded ittvrfidyg ntdiidnksv nirqipdswk
     1801 eikeysvkcr ldvipvdsed wnvttcekfe nlvtsveslq asiiansipk rvdllingks
     1861 isetlveehq avkihreeel ideivdleld phsafvshvn spsefwvqee ksvgdlevmt
     1921 drfivahmfp kveeikenll cvakypedgy wyrarvvshs dnatrviyid ygnsatstei
     1981 caipedlaii pplsrkcslv mpegitewsq raceeftkla adgatiflld vlkedetslv
     2041 kltlngenvt dilanfcerf ppiieerlpp lgeenspnvf vtrvnspddf wiqtesstad
     2101 lntmtdrlqa apsflplntf etgticaaky sedeqwyrak ilshsengte vlfidygnve
     2161 vtnetrvlpv diinipplak hcamqkpnsi sewsqemrkt feelaadget tfqfeilddt
     2221 ndpmhvklsl egtnvvdiit tytedessdv aiakedsvkd eninktadee thepvendea
     2281 reqeteenvn necsnmnssv eltideivqs mkpdeleeee tiesnninle tnenddvkkv
     2341 ttdleeqiei qssidvnqtq kteidteask ndletqnvne kevalnviev tkldsvstlv
     2401 stttdlqnpe eeqtnisgsk iesidnvrdt telespnkes vttdeieeic elqkdvqssn
     2461 lneentveve stseqsavtt dkieeicelk kdvqssnlne entvelespn kesvttdkie
     2521 eicelkkdkq dsnlneenii evestneqyv ttdkkdeire lkkdvessnl neentleves
     2581 tseqslttdk keeicelkkd vqssnlnkes taelespkke svttdkkeei celnkgiqnn
     2641 neentakles tseqhvtndn keetcelktn tqsnneente lkaaneqsvt ndkkeeicel
     2701 kkdiqssnfn eesiaelesp kkesvttdkk eeiceskkde rsnnlneess aklestsehh
     2761 vtndnkeetc glktdiqsnn eenselktan eqsvtsdtct evsqievvng deinneekks
     2821 dsencklqde nkdnvsttgp kdcseikadk vdkkecldvq fveiannecs kiidtpktis
     2881 lsdlsvstnv ekkkdnensl psegtsdsck
//
LOCUS       XP_034196258            2910 aa            linear   INV 03-SEP-2025
DEFINITION  protein tudor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196258
VERSION     XP_034196258.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340367.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196258.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2910
                     /product="protein tudor isoform X2"
                     /calculated_mol_wt=328660
     Region          <45..134
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          273..387
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          469..582
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          902..1013
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1121..1233
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1335..1447
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1512..1624
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1696..1811
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1893..1999
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          2075..2185
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <2255..2380
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     Region          2324..2833
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..2910
                     /gene="LOC117611909"
                     /coded_by="XM_034340367.2:213..8945"
                     /db_xref="GeneID:117611909"
ORIGIN      
        1 mniqknleev iifvthveae gpflkiwgqi dknsatcver milplveqft rsqgcigpla
       61 anlhinalcc arfqnegyyr arviniqpdg mvvlqfidyg nievlppqei hlldtipgse
      121 slksfppvaf dfalmnvlpi ndvwenkiie sikkslwyne ykiligsvvn nyrfiklwyn
      181 nedfsellik khmaigatlh dmfrpkciqq pppsncptmt pidnmnllrs dgnemhryqp
      241 nvplccatlq kqmmqapvqe alvfksrvld vpskhdvyvs fvedgphkfs vhlistseil
      301 rvlmreinah pleplqeppl pgsvclgrft qdkvlcravv msvtdskckl yyvdfghtel
      361 lpytdifqlp phfinprvls irftlsgvye lnatdemkqy fkdivsgkpl vlhvrppegp
      421 plvqygdlyd neknikdilr qafptpgtmt paaaltyqep kkllkgteed vlvsfvesck
      481 kffvqlqnnv kslelvmnhl aefcktapil slshlniglp caalydnqwy raqiiginqn
      541 sikvlyvdyg neetvplssl rfiredlvkk lsaqaikcvl ngwgllpctq elydqfelmi
      601 lekslrlrvv dvtangvvvd lyepetmedi ksqmlqpted ekkvinqpny etmeeqqpvk
      661 ispkmnqses tnkwhkkdqs dswrqppnrd faqeknkfkt wkdesnegkl ndarndkgnf
      721 hrndtrnerf nrddfsnnrn trdkwnnkne yndsfkggrs prgggrnkts gyggfknhss
      781 sdkswsdkds dtsskgssrr grngstrgys kregfsgriq rdkysdkend rsfrggktns
      841 dfsrgndrfr dkkgqgyrls qnklnavnsf sdgeprrysp mrnrnevrip spnidigstk
      901 tcevvfttsl sdffiqlspd ytaldsvmen iasiyeegge llkeseilsg vyciaqysvd
      961 lkwyraliks vegnsatvqf vdygnietve fdkikviqee flklpaqavh ckllglkdda
     1021 ldnsrvkhfe dkvtektlea efiieengry cvllrevieg wptntfinee fcknidlqka
     1081 kedalfsrkt spttkqfnlp dyassdakwt tisdilgttk daiitwftnp nnfycqvldn
     1141 etefrtmmne iqkiyvgrep vpsytlqigs sviaifsedg alyraeilel nklnghlvqy
     1201 idfgnsavvn tnkiypvekk lmhlpkqavh csllnivpkd nqgwlkinta aidncfnadr
     1261 fecvfhdlkd dkylislsnn gndvaatlve kdlatfases ksdaavegsd tttsavspnv
     1321 ervdinllsg qvlrvkvssv enaskfyvql psasecetri nsymankdpe vmqrlaahei
     1381 clgtgcliys ngvwrravin nhsrtagfdv kfidtgacde ilsdsvlalp gelsvmqnqa
     1441 iecsladvvs ssetdnalkk haegkeviic vnevdnnrli vkvfdlfgnk ikvsedsdek
     1501 ispicpmpil ssthkvsvsy adhsasiwlq rnaeyildtn lsealdqhys tsgklieptv
     1561 glvcaaksvd ghwyrakvik ctengvyvnf idygnneevt sdvvtalepc fyiphqlain
     1621 vslpvtlncp ipeqlsvlqn ylsnkeltav frntnnkwvv ellddgekis dkfrslnaee
     1681 lsstgkpyih nmtvgstynv yvshtdspsq fwlqhvddat elstkqeelq eqaptfpave
     1741 gileegslcv avytidnlwy raqvldaded ittvrfidyg ntdiidnksv nirqipdswk
     1801 eikeysvkcr ldvipvdsed wnvttcekfe nlvtsveslq asiiansipk rvdllingks
     1861 isetlveehq avkihreeel ideivdleld phsafvshvn spsefwvqee ksvgdlevmt
     1921 drfivahmfp kveeikenll cvakypedgy wyrarvvshs dnatrviyid ygnsatstei
     1981 caipedlaii pplsrkcslv mpegitewsq raceeftkla adgatiflld vlkedetslv
     2041 kltlngenvt dilanfcerf ppiieerlpp lgeenspnvf vtrvnspddf wiqtesstad
     2101 lntmtdrlqa apsflplntf etgticaaky sedeqwyrak ilshsengte vlfidygnve
     2161 vtnetrvlpv diinipplak hcamqkpnsi sewsqemrkt feelaadget tfqfeilddt
     2221 ndpmhvklsl egtnvvdiit tytedessdv aiakedsvkd eninktadee thepvendea
     2281 reqeteenvn necsnmnssv eltideivqs mkpdeleeee tiesnninle tnenddvkkv
     2341 ttdleeqiei qssidvnqtq kteidteask ndletqnvne kevalnviev tkldsvstlv
     2401 stttdlqnpe eeqtnisgsk iesidnvrdt telespnkes vttdeieeic elqkdvqssn
     2461 lneentveve stseqsavtt dkieeicelk kdvqssnlne entvelespn kesvttdkie
     2521 eicelkkdkq dsnlneenii evestneqyv ttdkkdeire lkkdvessnl neentleves
     2581 tseqslttdk keeicelkkd vqssnlnkes taelespkke svttdkkeei celnkgiqnn
     2641 neentakles tseqhvtndn keetcelktn tqsnneente lkaaneqsvt ndkkeeicel
     2701 kkdiqssnfn eesiaelesp kkesvttdkk eeiceskkde rsnnlneess aklestsehh
     2761 vtndnkeetc glktdiqsnn eenselktan eqsvtsdtct evsqievvng deinneekks
     2821 dsencklqde nkdnvsttgp kdcseikadk vdkkecldvq fveiannecs kiidtpktis
     2881 lsdlsvstnv ekkkdnensl psegtsdsck
//
LOCUS       XP_034196261            2887 aa            linear   INV 03-SEP-2025
DEFINITION  protein tudor isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034196261
VERSION     XP_034196261.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340370.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196261.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2887
                     /product="protein tudor isoform X3"
                     /calculated_mol_wt=326560
     Region          <77..166
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          305..419
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          501..614
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          934..1045
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1153..1265
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1489..1601
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1673..1788
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          1870..1976
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          2052..2162
                     /region_name="TUDOR"
                     /note="Tudor domain; pfam00567"
                     /db_xref="CDD:425754"
     Region          <2232..2357
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     Region          2301..2810
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..2887
                     /gene="LOC117611909"
                     /coded_by="XM_034340370.2:94..8757"
                     /db_xref="GeneID:117611909"
ORIGIN      
        1 mkakscrknh rfiyvitqkl vhhirghkfy namniqknle eviifvthve aegpflkiwg
       61 qidknsatcv ermilplveq ftrsqgcigp laanlhinal ccarfqnegy yrarviniqp
      121 dgmvvlqfid ygnievlppq eihlldtipg seslksfppv afdfalmnvl pindvwenki
      181 iesikkslwy neykiligsv vnnyrfiklw ynnedfsell ikkhmaigat lhdmfrpkci
      241 qqpppsncpt mtpidnmnll rsdgnemhry qpnvplccat lqkqmmqapv qealvfksrv
      301 ldvpskhdvy vsfvedgphk fsvhlistse ilrvlmrein ahpleplqep plpgsvclgr
      361 ftqdkvlcra vvmsvtdskc klyyvdfght ellpytdifq lpphfinprv lsirftlsgv
      421 yelnatdemk qyfkdivsgk plvlhvrppe gpplvqygdl ydneknikdi lrqafptpgt
      481 mtpaaaltyq epkkllkgte edvlvsfves ckkffvqlqn nvkslelvmn hlaefcktap
      541 ilslshlnig lpcaalydnq wyraqiigin qnsikvlyvd ygneetvpls slrfiredlv
      601 kklsaqaikc vlngwgllpc tqelydqfel milekslrlr vvdvtangvv vdlyepetme
      661 diksqmlqpt edekkvinqp nyetmeeqqp vkispkmnqs estnkwhkkd qsdswrqppn
      721 rdfaqeknkf ktwkdesneg klndarndkg nfhrndtrne rfnrddfsnn rntrdkwnnk
      781 neyndsfkgg rsprgggrnk tsgyggfknh sssdkswsdk dsdtsskgss rrgrngstrg
      841 yskregfsgr iqrdkysdke ndrsfrggkt nsdfsrgndr frdkkgqgyr lsqnklnavn
      901 sfsdgeprry spmrnrnevr ipspnidigs tktcevvftt slsdffiqls pdytaldsvm
      961 eniasiyeeg gellkeseil sgvyciaqys vdlkwyrali ksvegnsatv qfvdygniet
     1021 vefdkikviq eeflklpaqa vhckllglkd daldnsrvkh fedkvtektl eaefiieeng
     1081 rycvllrevi egwptntfin eefcknidlq kakedalfsr ktspttkqfn lpdyassdak
     1141 wttisdilgt tkdaiitwft npnnfycqvl dnetefrtmm neiqkiyvgr epvpsytlqi
     1201 gssviaifse dgalyraeil elnklnghlv qyidfgnsav vntnkiypve kklmhlpkqa
     1261 vhcsllnivp kdnqgwlkin taaidncfna drfecvfhdl kddkylisls nngndvaatl
     1321 vekdlatfas esksdaaveg sdtttsavsp nvervdinll sgqvlrvkvs svenaskfyv
     1381 qlpsasecet rinsymankd pevlalpgel svmqnqaiec sladvvssse tdnalkkhae
     1441 gkeviicvne vdnnrlivkv fdlfgnkikv sedsdekisp icpmpilsst hkvsvsyadh
     1501 sasiwlqrna eyildtnlse aldqhystsg klieptvglv caaksvdghw yrakvikcte
     1561 ngvyvnfidy gnneevtsdv vtalepcfyi phqlainvsl pvtlncpipe qlsvlqnyls
     1621 nkeltavfrn tnnkwvvell ddgekisdkf rslnaeelss tgkpyihnmt vgstynvyvs
     1681 htdspsqfwl qhvddatels tkqeelqeqa ptfpavegil eegslcvavy tidnlwyraq
     1741 vldadeditt vrfidygntd iidnksvnir qipdswkeik eysvkcrldv ipvdsedwnv
     1801 ttcekfenlv tsveslqasi iansipkrvd llingksise tlveehqavk ihreeelide
     1861 ivdleldphs afvshvnsps efwvqeeksv gdlevmtdrf ivahmfpkve eikenllcva
     1921 kypedgywyr arvvshsdna trviyidygn satsteicai pedlaiippl srkcslvmpe
     1981 gitewsqrac eeftklaadg atiflldvlk edetslvklt lngenvtdil anfcerfppi
     2041 ieerlpplge enspnvfvtr vnspddfwiq tesstadlnt mtdrlqaaps flplntfetg
     2101 ticaakysed eqwyrakils hsengtevlf idygnvevtn etrvlpvdii nipplakhca
     2161 mqkpnsisew sqemrktfee laadgettfq feilddtndp mhvklslegt nvvdiittyt
     2221 edessdvaia kedsvkdeni nktadeethe pvendeareq eteenvnnec snmnssvelt
     2281 ideivqsmkp deleeeetie snninletne nddvkkvttd leeqieiqss idvnqtqkte
     2341 idteaskndl etqnvnekev alnvievtkl dsvstlvstt tdlqnpeeeq tnisgskies
     2401 idnvrdttel espnkesvtt deieeicelq kdvqssnlne entvevests eqsavttdki
     2461 eeicelkkdv qssnlneent velespnkes vttdkieeic elkkdkqdsn lneeniieve
     2521 stneqyvttd kkdeirelkk dvessnlnee ntlevestse qslttdkkee icelkkdvqs
     2581 snlnkestae lespkkesvt tdkkeeicel nkgiqnnnee ntaklestse qhvtndnkee
     2641 tcelktntqs nneentelka aneqsvtndk keeicelkkd iqssnfnees iaelespkke
     2701 svttdkkeei ceskkdersn nlneessakl estsehhvtn dnkeetcglk tdiqsnneen
     2761 selktaneqs vtsdtctevs qievvngdei nneekksdse ncklqdenkd nvsttgpkdc
     2821 seikadkvdk kecldvqfve iannecskii dtpktislsd lsvstnvekk kdnenslpse
     2881 gtsdsck
//
LOCUS       XP_034196262            1256 aa            linear   INV 03-SEP-2025
DEFINITION  putative RNA helicase armitage [Osmia lignaria lignaria].
ACCESSION   XP_034196262
VERSION     XP_034196262.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196262.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1256
                     /product="putative RNA helicase armitage"
                     /calculated_mol_wt=142270
     Region          777..1025
                     /region_name="DEXXQc_Mov10L1"
                     /note="DEXXQ-box helicase domain of Mov10L1; cd18078"
                     /db_xref="CDD:350836"
     Site            order(781,805..810,972,1024..1025)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350836"
     Region          <907..1222
                     /region_name="DNA2"
                     /note="Superfamily I DNA and/or RNA helicase [Replication,
                     recombination and repair]; COG1112"
                     /db_xref="CDD:440729"
     CDS             1..1256
                     /gene="armi"
                     /coded_by="XM_034340371.2:171..3941"
                     /db_xref="GeneID:117611910"
ORIGIN      
        1 mfsliynvik ytmgyksant sdtdeinnvi ariesahkee iqkdeylsnh dycyktgnit
       61 yiskdyvvvd hshmcdrani svnnlkvgdt vqclvsqnnh eneykiqqii smtddswddv
      121 vtdpqrnive pktlikcivg kvidrknrli ivepknisvd lnkvqsefip vigdwlklda
      181 eveinensdn lndiilkiqk iqplrskldh gtvssydsik eigsigkdiv fnkricesgy
      241 ipcvgdkvis dsiesdqgiy twrslvvvpl fqvpgketkl pilcntfrtn rnldelmkdk
      301 cgivisnnln ieldvleeqt livtvqnvgn tthvlrkgcf mskrnqsqla lmqpptlntv
      361 svlnpsdelt yifkcaakfi gtseelfifn fkdfeigrvf rinvrpknvq sktcvksdni
      421 kanksgqild ldewsqmtyi pgirpckppa fikvrnvifk vprhywnvis kciderksqt
      481 eceydignai pcllnrlsfe vykdrfhall yleeiaqtis mqqynlestv mrragdylvm
      541 evpglaekrp sliigdraiv sfkwdntqgq lkyegfihri tntdiflkfn ykfhqdynye
      601 dcqvtfkcsn savqrchnai nmavhrlgsn flfptyviek esqvdleefe tndkpvskql
      661 ghqrndsvss esstsstpts nkssntsrss tkisvvqrlf nvkpiqenke cetssitltk
      721 nnqdvethkk tdvdknlldk tlsrnsrets vssyntelqp yitqikkrkl iwfnqnlnyy
      781 qkeavknilk gharplpyvi fgppgtgkti tlcetilqil stipesrlli atpsnssanl
      841 iaerlldsgv lkpgdmvrli ahhylssdsi sgkllpycat aeiadeksva ktryngegpk
      901 lnctmsiigr hritvgtcia lgilnnmgfp rghfshvlid eagqatepei miplnfihfd
      961 ygqvilagdp lqlgpvvqsr laknfgldes fltrllrhfp yqkdpngfes qydprlvtkl
     1021 vinyrslpei lelssslfyd seliaqispk kskeakllqt laselperng appaiifhgv
     1081 ngenckdsds pswynpeeat qaylyllkly kcglspddlg iitpyqkqvy qirellmeln
     1141 vdlpkissve gfqgqernvi iisavrsssn finedikhsl gfvacprrln vaitraralv
     1201 iilgnpylla qdpywrsvli ycinrnsytg ctftlsdidd aqteistdtt dkmnnl
//
LOCUS       XP_034196263             437 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 3 subunit E [Osmia
            lignaria lignaria].
ACCESSION   XP_034196263
VERSION     XP_034196263.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340372.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..437
                     /product="eukaryotic translation initiation factor 3
                     subunit E"
                     /calculated_mol_wt=51101
     Region          3..422
                     /region_name="eIF3E"
                     /note="eukaryotic translation initiation factor 3 subunit
                     E; cd21378"
                     /db_xref="CDD:411062"
     Site            order(4,7..8,11..12,16,245..247,269,279,282..283,299..301,
                     303,344..348,366..367,370,373..375,380..385,393..395)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:411062"
     Site            order(399..400,402..403,406..407,410..411,413..415,418)
                     /site_type="other"
                     /note="helix bundle interface [polypeptide binding]"
                     /db_xref="CDD:411062"
     CDS             1..437
                     /gene="eIF3e"
                     /coded_by="XM_034340372.2:102..1415"
                     /db_xref="GeneID:117611911"
ORIGIN      
        1 makfdltsri gqyldrhlvf plleflsakq iydedellqa kldilsktnm idytidirkq
       61 lypnlevpee lkarradvlq elgylqnnvs vvvalmnnee vmkkmenmrd skalnnyltq
      121 esdfrvemmd sfvklakyry ecgnysvsts ylyfymlims ptdknylnvl wgklaseilv
      181 qnwetaledv nklreyidsn vignslqvlq qrtwlihwsl fvffnhvkgr dliiemflyr
      241 phylnaiqtm cphilrylaa avivnrsrrs ilkdlvkviq qesytyrdpi teflehlyvn
      301 fdfdgarqkl qecqtvvfnd ffliallsef venarlmife tfcrihqcis igmlaeklnm
      361 kadvaecwiv nlirnarlda kidsklghvv mggqpaspyq qlvekietls vrsealenli
      421 erklkaknqd pvsivwn
//
LOCUS       XP_034196264             205 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611912 [Osmia lignaria lignaria].
ACCESSION   XP_034196264
VERSION     XP_034196264.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340373.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..205
                     /product="uncharacterized protein LOC117611912"
                     /calculated_mol_wt=23045
     CDS             1..205
                     /gene="LOC117611912"
                     /coded_by="XM_034340373.2:218..835"
                     /db_xref="GeneID:117611912"
ORIGIN      
        1 mkkggtsrkh hvatskqnll lkpygfccdv kdnpgfskhk vsilpflsrg ttnegpdikf
       61 rinedelsss glrggqkgfq kggrgrnagr rrsvytnglr ggyqnrlrgg ndnhagtsrg
      121 galgplndrl vcercggplr wyledeiava resmvkkrie kyntdmnrnv qtakrkeknk
      181 yghkkrrrqq ssedkehkqe sedkt
//
LOCUS       XP_034196248            3053 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611905 [Osmia lignaria lignaria].
ACCESSION   XP_034196248
VERSION     XP_034196248.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340357.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196248.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3053
                     /product="uncharacterized protein LOC117611905"
                     /calculated_mol_wt=346833
     Region          <381..821
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          643..1471
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     Region          <1333..>1561
                     /region_name="2A1904"
                     /note="K+-dependent Na+/Ca+ exchanger; TIGR00927"
                     /db_xref="CDD:273344"
     Region          <2195..>2587
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..3053
                     /gene="LOC117611905"
                     /coded_by="XM_034340357.2:585..9746"
                     /db_xref="GeneID:117611905"
ORIGIN      
        1 mkiptillsl ffccvavsse iqqqkksngq tekrvgvett vkrshvtdve aasrnvqtkr
       61 slevgqqksr skeaglsfqe tgsaqktssg tdwggllpda eekdrkkqse vspiiawlgn
      121 lrasksneqi slhrgpywds ftsedakytr ldqaeklgdk pindnwymyl ipessedyse
      181 slapgrsaii qsqqrngqwi ssgtnlrwke gpewsnrgkk iknedasarw rvqklptsqw
      241 ntdwkkenlk eikgnegsri dwkrernsqw knsgldvknw kinednskks egssrslegl
      301 rsksekslds sddrkaneeg erinkylkhk tlfdsdplll ldelehgsky khkgtgrydt
      361 mlhghkahdk myesnryign rrgsekvdel pemlklkgwr eedekmkaer ehsskkiqle
      421 kpsketsddr iktlwstrqs dkiqgkndek hrilvinkki pdeklkshel kswkskilag
      481 eqrnqysqrg edktisseek ksekdesgga rshmwksnme qkvpqrnres kekeennlqk
      541 ekiaagksgi edlgkqehgp rgwkmykplk dskekistgd gfevrkwsae ipdenrhwkq
      601 nlrqqvlkte dagwwkpssd hwnnkglkta speqqmlglq niernnhime vsqnkeleed
      661 srkkneeeen iktgrwesek iekqtteivn yglkesdkek pemkdlllkk sksetaekkk
      721 iqeqreeksp eekktkklei hkpedqgwnq kekseqsekk evhvesenkg lrkielkeie
      781 vekpqgqgwt migkpeetek kevqierekq glgekevkve kpqelgwami ekpeeiekke
      841 vqveskkkgl geiaikeiev akpqgqgwam idkpeeteki evtvqsekqg lgeielkeiq
      901 vekpqelgwa mqkkpeeskk kevevenekq glgekevkvq kpqeqewtii gkpealkkke
      961 vqvegekqgl gdkevkvekp qeqgwamigk skalekkddq vegekqglgd kelkvekpqe
     1021 qgwarivkpe qsekkeveve sdrkglgeie lqeieldmpq eqgwaiiekp eeskkkeaqv
     1081 egekqglgek evkvqkpqkq ewamigkpea lekkddqveg ekqglgdkdv kvekpqeqgw
     1141 aiiekpeksk keeaqvegek qglgekevkv qkpqkqewam igkpealekk ddqvegekqg
     1201 lgdkevkvak pqeqgwaiie kpeeiekkev qvegerqglg ekevkvrkpq eqewamigkp
     1261 ealekkevqv kgekqglgdk evkvakpqeq gwaiiekpee iekkevqveg erqglgekev
     1321 kvrkpqeqew amigkpeale kkevqvkgek qglgdkevnv ekpqeqgwar ivkpeqsekk
     1381 evevesdrkg lgeielkeie ldmpqeqrwa miekpeetee mkvtvesgkq glgerelkei
     1441 qvekpqeqdw amiekpeqtg kkeiqvesek qglgenevkv ekpqeqgwai iekpketekk
     1501 evqvegdkqg lggkevkvqk pqeqewamig kpealekkev qvegekqglg nkevnvekpq
     1561 eqgwariekp eesekkevev esdrkglgei elteielekp qeqgwpmigk peesektele
     1621 vqsdwkglge ielkkielgk pqeqrwamie kpeetekmkv tvesgkqalg eielkeiqve
     1681 kpqeqgwdkk ekpaesekke vqveserngl geieskeigv eksqerewie edvdagkgkl
     1741 lnsvkkelkv egleknlmee gvkmkglksn qskkqsieig evglgdskev kmeakdvein
     1801 ksiskdsekv mlepedlkgg ktvrweeqgl eagipketnp gtesllwspe rgflwssnph
     1861 tteavpdgld eknlpredse kkesgpsvle alnpwepsdi krneelqlpe snmwrrkgwa
     1921 dinipaqkdd kestllgked kndqsltnnk llqkkpikia wpetsnerss ksketgssie
     1981 ntylkasgdl qksdlsakkv iakkesnedi aiskesglil knskegekls kqsenngeiw
     2041 rklnkndlts dnglikpanv ktitleqnmn kdetmvpdkn mgqagslsti gsiltgspss
     2101 sqrsdvssdt sveanmklgd ankairwrgg ntakaqsaen vnndgtaaai tndastgadn
     2161 qdkievsdlh pdlsksreat hqmvphsakn nqhplkipre eseieilkpf eenkqrivae
     2221 dksgierlld tgvkqdslkk ktkeetsatd lisnqgktll tasksegdiv esekveegtr
     2281 etekvahekd enykiledqn rkntektmet gtneigqvss kkkaeetkei thglnekgnw
     2341 kqgttelsvn kvdnkvdlie nqdkdsrkmn mwkqlslaqd vsskseeeit skssetikqg
     2401 skiasskewm itdkklqtsk erqvtksier ndnaskerga ssvvinqvak eesqvektse
     2461 qnvaedhlsk qrnskvisnl ssekkgredk veeevsptsk vsnvelksas stkdsvtelp
     2521 kggnirvikd glrpeesdth vwkqqkdeke vspnesesik rlwdtkkasr gdvksaegnn
     2581 vseekeqssl ashalipgkn epwspvlgvk sleendkssw nklnlpgdsr etklnangma
     2641 asnlrisagk qliednkqev psiisspaal ntkttgkarp pvanedsivr ylnevvgath
     2701 tnefesprie gtirkgklgn wapiplkkqq sgmisdkkdi eiekeetkas sksediikdd
     2761 kvknwapipl tkqpslnwnn kedieiekqe tkgsskgegi irndqvkkwp pnplkkqpsi
     2821 nwnnkgdvel eklgikeplk neeiirndqi kkwtpkplak pvsvkdtsil nvpmaqksek
     2881 qiskeedqkl vndelsdedw ntgivdklhp asrnfakplr kdvnknewpe sweslksgsn
     2941 kslendswte tvhrmirsll wiirswriek tvsdesdtkt snaeiikets kesdttnldv
     3001 yvvkwgqkgp vkvaavnnmk psiinklllp rksyeeires ykknaiknip vaa
//
LOCUS       XP_034196252             755 aa            linear   INV 03-SEP-2025
DEFINITION  protein O-mannosyl-transferase TMTC1-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034196252
VERSION     XP_034196252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340361.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..755
                     /product="protein O-mannosyl-transferase TMTC1-like"
                     /calculated_mol_wt=84815
     Region          273..347
                     /region_name="TMTC_DUF1736"
                     /note="Protein O-mannosyl-transferase TMTC, DUF1736;
                     pfam08409"
                     /db_xref="CDD:462468"
     Region          428..673
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          464..492
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(465,468..469,472..473,475,499,502..503,506,507..508,
                     529,532..533,536..537,540)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          497..523
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          528..556
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..755
                     /gene="LOC117611906"
                     /coded_by="XM_034340361.2:3224..5491"
                     /db_xref="GeneID:117611906"
ORIGIN      
        1 mrrrnnsnvg cccqsnkrsg ragyndadgn eppgwclyts valvavacyl nalngdfvhd
       61 dipavvrnkd vlaqtpwtsv lkddfwgtpm hdinshksyr plttltfrln ylmsgltpsw
      121 yhatnialha iacvlvtrvs lvvaalrpgf aalagllfaa hpvhteavtg ivgradvlac
      181 iffllsflay hgqqtayvws svclgalsml aketgitvlp lnllydlcrs whsikrsife
      241 arwnddsrhf srraavllvs fgvllvvrla llhgalpkfs pqdnpaafhp cfhvrlltfc
      301 ylaalncwll lcpatlshdw qmgsvplvas ladtrnlatc lffggclilt ykaftdfeqq
      361 rhpplllgwm flvlpflpas nlfitvgfvv aervlympsv gwillvvygm qiswtaiprr
      421 rslittgivl llllgcyrtv lrnrdwtsre tllraglrsl phnakmhynf anflrdmsqp
      481 nrailhyqla lwlwptyasa hnnlgtltvg dqaeqhflaa ihaqpghvna hynlgqlyrk
      541 tnrteecirm lqkcvsldpa ytpaylvlar latgpaagvl lrhvvrlqpr spdhlaeyas
      601 wlhqngkwlp slkyylkame vspshrssll gtvrilrsrg qwprvhqlit rwhlmlrakr
      661 ggliyrgdly irawqlqses rrrahqhqan iclseggcst prvasvsvql eqdsnkseql
      721 eraprcnvrt crqsrkgktr vtapallvqn lletl
//
LOCUS       XP_034196255             236 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611907 [Osmia lignaria lignaria].
ACCESSION   XP_034196255
VERSION     XP_034196255.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340364.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196255.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="uncharacterized protein LOC117611907"
                     /calculated_mol_wt=25650
     CDS             1..236
                     /gene="LOC117611907"
                     /coded_by="XM_034340364.2:8901..9611"
                     /db_xref="GeneID:117611907"
ORIGIN      
        1 mwcrlivlff ifccsiealg fddvvsvesl rrdvreplpf atevedllgq gnspandras
       61 kkhmknvsgk sdeggyykty gsdaegekgy lkatfskgnh gyknldtfhk qdgdkyafek
      121 hiaygkaqad kksghnvaaa srsskggdye gagtivdshy tadegdhgdh sgehvegdhs
      181 gysegahytd hgpesssygh segyatkdgd hgsyeshssy srdygdeqgd qgghyy
//
LOCUS       XP_034195805            1053 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611737 [Osmia lignaria lignaria].
ACCESSION   XP_034195805
VERSION     XP_034195805.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339914.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195805.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1053
                     /product="uncharacterized protein LOC117611737"
                     /calculated_mol_wt=119205
     CDS             1..1053
                     /gene="LOC117611737"
                     /coded_by="XM_034339914.2:755..3916"
                     /db_xref="GeneID:117611737"
ORIGIN      
        1 mqkrirvqnl lliaaalatc vsivasssps snrerrhvgv eesshrpnkt hhtptgrsfk
       61 sstnryneit tepgrkvthr ligghlrada khendetfwd dgillssagn gkegkpnrkd
      121 dddekktlsq qvkegkygli qdeiyekqpk rpgiisylgn pevpkdtikn lggldeddiw
      181 laenhvlvlr ggkfpghest qkdgqtwppi dnykaprrqv kipsrpkipp pfpvqltegg
      241 pvqiigtngt kdivngsvdy kkdplltkgl lpedgplfav isngtiltsd qknatsneek
      301 peslppgnfp pfyhaippga vfvpppvnls dydeedqsiy ypppysfqyq qdnttavppg
      361 plvpgiilpp ppdffspled kkpttkkytk rpgtttsprp rptylpprkl ttkqfksttt
      421 vpttksrssm ttntkgygrt syknltdast prmktklyie vttpksdkpt tldnaelysv
      481 spvienqvtn qenvqekkqa wpglvsktnp ivayyatttq stlerpvevt patvknivtt
      541 dtpvrsnsqa syyfyeesnd epigttanps sfyyktttes pfyetvtrpr ledkkkqyys
      601 vetipsqets kdysikfigs lvknsgpvry tdstvtrtss nyetsgsart qgqrmlpdha
      661 plyyqtisar pavpvqpyyt takppqtyyr qetkpkpiyq ysfeaadysk rgdhqrsryq
      721 qhrqqtntye gypnvkvski gyddhrndyq qdesvvekrv rpesvpyksq qpiytptshs
      781 listtpnphh vyytqqddkf ldevtkeyft mfgkkiahkn lpsttpiypk snsvterpdy
      841 ntntyvenny ntaeapiykt pnvkvhygdq sqrpyslkdd ilvnyrqplp piepdsefia
      901 vidpsqqppn yrladredqp fasirayppq qlvntrndas tnyvpivesp eeiderypql
      961 pisladdikv nyrdprptin pdaefidplp vrdsqeekqa kayfayrlpg dgghfyfltp
     1021 qaitqrpeqn gshlysksrg trllrrrrwp anv
//
LOCUS       XP_034195806            1053 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611737 [Osmia lignaria lignaria].
ACCESSION   XP_034195806
VERSION     XP_034195806.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339915.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195806.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1053
                     /product="uncharacterized protein LOC117611737"
                     /calculated_mol_wt=119205
     CDS             1..1053
                     /gene="LOC117611737"
                     /coded_by="XM_034339915.2:1982..5143"
                     /db_xref="GeneID:117611737"
ORIGIN      
        1 mqkrirvqnl lliaaalatc vsivasssps snrerrhvgv eesshrpnkt hhtptgrsfk
       61 sstnryneit tepgrkvthr ligghlrada khendetfwd dgillssagn gkegkpnrkd
      121 dddekktlsq qvkegkygli qdeiyekqpk rpgiisylgn pevpkdtikn lggldeddiw
      181 laenhvlvlr ggkfpghest qkdgqtwppi dnykaprrqv kipsrpkipp pfpvqltegg
      241 pvqiigtngt kdivngsvdy kkdplltkgl lpedgplfav isngtiltsd qknatsneek
      301 peslppgnfp pfyhaippga vfvpppvnls dydeedqsiy ypppysfqyq qdnttavppg
      361 plvpgiilpp ppdffspled kkpttkkytk rpgtttsprp rptylpprkl ttkqfksttt
      421 vpttksrssm ttntkgygrt syknltdast prmktklyie vttpksdkpt tldnaelysv
      481 spvienqvtn qenvqekkqa wpglvsktnp ivayyatttq stlerpvevt patvknivtt
      541 dtpvrsnsqa syyfyeesnd epigttanps sfyyktttes pfyetvtrpr ledkkkqyys
      601 vetipsqets kdysikfigs lvknsgpvry tdstvtrtss nyetsgsart qgqrmlpdha
      661 plyyqtisar pavpvqpyyt takppqtyyr qetkpkpiyq ysfeaadysk rgdhqrsryq
      721 qhrqqtntye gypnvkvski gyddhrndyq qdesvvekrv rpesvpyksq qpiytptshs
      781 listtpnphh vyytqqddkf ldevtkeyft mfgkkiahkn lpsttpiypk snsvterpdy
      841 ntntyvenny ntaeapiykt pnvkvhygdq sqrpyslkdd ilvnyrqplp piepdsefia
      901 vidpsqqppn yrladredqp fasirayppq qlvntrndas tnyvpivesp eeiderypql
      961 pisladdikv nyrdprptin pdaefidplp vrdsqeekqa kayfayrlpg dgghfyfltp
     1021 qaitqrpeqn gshlysksrg trllrrrrwp anv
//
LOCUS       XP_034195813             861 aa            linear   INV 03-SEP-2025
DEFINITION  N-alpha-acetyltransferase 15/16 [Osmia lignaria lignaria].
ACCESSION   XP_034195813
VERSION     XP_034195813.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339922.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="N-alpha-acetyltransferase 15/16"
                     /calculated_mol_wt=100366
     Region          13..39
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          45..290
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(48,51..52,55..56,58,82,85..86,89..90,92..93,116,
                     119..120,123..124,127)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          48..75
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          80..110
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          149..173
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          185..697
                     /region_name="NatA_aux_su"
                     /note="N-terminal acetyltransferase A, auxiliary subunit;
                     pfam12569"
                     /db_xref="CDD:463630"
     Region          375..401
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(377..378,381..382,384,408,411..412,415..416,
                     418..419,442,445..446,449..450,453)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          406..436
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          441..466
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..861
                     /gene="Naa15-16"
                     /coded_by="XM_034339922.2:285..2870"
                     /db_xref="GeneID:117611739"
ORIGIN      
        1 mpssnplppk enalfkrilr cyehkqykng ikfakqilsn pkfsehgetl amkgltlncl
       61 grkeeaydhv rrglrndlqs hvcwhvygll qrsdkkydea ikcyrnalkw dkdniqilrd
      121 lsllqiqmrd ldgykdtryq lfmlrptqra swigfaisyh llkdyemalk ildtfrnspm
      181 icydyehsel llyenmviqe sgeceqalkh ldkysdqicd kvtvketygk lrlqlkqyaa
      241 aaqvykelln inpenttyya rlaeaerhtk pdetlatlqr yeelfprala prrlqlnyav
      301 gdefkalvdr ylrrglhkgv pplfvnlrsl ysdqqkvdii qslvleykea lkvhghfsdq
      361 erdnprepas allwtyyyla qhydhlglte kalneidaai ehtptlielf vtkgriykha
      421 gdvqeaykwl deaqgldtad ryinskcaky mlranlikea eetcgkftre gvlamenlne
      481 mqcmwiqtea anaykrlgky gealkkchev drhfseiied qfdfhtycmr kmtlrsyvgl
      541 lrledvlrsh pfyfkaaqca ievylrlfdd plpdptqsqe idtenlapse lkklrnkqrk
      601 qrrkaelkrq qaaqaqekre qhnksrqqad pdleqptlde lipeklerve dpleqaikfl
      661 kplqelasdr iethlmafei yhrkcktllm lrsikrahnl dpnhpdlhtc ivnfilhish
      721 lglekpvgev vkrqtagiys tsstmyftlf hfnaeflkkn rnslphllqa akimdfldms
      781 pvleetalsv vtnidnlegv tlenctkvle smrngdfgdc daaiadfmak chkrfpyata
      841 frppetkatt nhqekensik n
//
LOCUS       XP_076546820             861 aa            linear   INV 03-SEP-2025
DEFINITION  N-alpha-acetyltransferase 15/16 [Osmia lignaria lignaria].
ACCESSION   XP_076546820
VERSION     XP_076546820.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690705.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..861
                     /product="N-alpha-acetyltransferase 15/16"
                     /calculated_mol_wt=100366
     Region          13..39
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          45..290
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Site            order(48,51..52,55..56,58,82,85..86,89..90,92..93,116,
                     119..120,123..124,127)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          48..75
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          80..110
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          149..173
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          185..697
                     /region_name="NatA_aux_su"
                     /note="N-terminal acetyltransferase A, auxiliary subunit;
                     pfam12569"
                     /db_xref="CDD:463630"
     Region          375..401
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(377..378,381..382,384,408,411..412,415..416,
                     418..419,442,445..446,449..450,453)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          406..436
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          441..466
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..861
                     /gene="Naa15-16"
                     /coded_by="XM_076690705.1:285..2870"
                     /db_xref="GeneID:117611739"
ORIGIN      
        1 mpssnplppk enalfkrilr cyehkqykng ikfakqilsn pkfsehgetl amkgltlncl
       61 grkeeaydhv rrglrndlqs hvcwhvygll qrsdkkydea ikcyrnalkw dkdniqilrd
      121 lsllqiqmrd ldgykdtryq lfmlrptqra swigfaisyh llkdyemalk ildtfrnspm
      181 icydyehsel llyenmviqe sgeceqalkh ldkysdqicd kvtvketygk lrlqlkqyaa
      241 aaqvykelln inpenttyya rlaeaerhtk pdetlatlqr yeelfprala prrlqlnyav
      301 gdefkalvdr ylrrglhkgv pplfvnlrsl ysdqqkvdii qslvleykea lkvhghfsdq
      361 erdnprepas allwtyyyla qhydhlglte kalneidaai ehtptlielf vtkgriykha
      421 gdvqeaykwl deaqgldtad ryinskcaky mlranlikea eetcgkftre gvlamenlne
      481 mqcmwiqtea anaykrlgky gealkkchev drhfseiied qfdfhtycmr kmtlrsyvgl
      541 lrledvlrsh pfyfkaaqca ievylrlfdd plpdptqsqe idtenlapse lkklrnkqrk
      601 qrrkaelkrq qaaqaqekre qhnksrqqad pdleqptlde lipeklerve dpleqaikfl
      661 kplqelasdr iethlmafei yhrkcktllm lrsikrahnl dpnhpdlhtc ivnfilhish
      721 lglekpvgev vkrqtagiys tsstmyftlf hfnaeflkkn rnslphllqa akimdfldms
      781 pvleetalsv vtnidnlegv tlenctkvle smrngdfgdc daaiadfmak chkrfpyata
      841 frppetkatt nhqekensik n
//
LOCUS       XP_034195815             508 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase III subunit C [Osmia lignaria lignaria].
ACCESSION   XP_034195815
VERSION     XP_034195815.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339924.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="RNA polymerase III subunit C"
                     /calculated_mol_wt=58948
     Region          15..72
                     /region_name="HTH_9"
                     /note="RNA polymerase III subunit RPC82 helix-turn-helix
                     domain; pfam08221"
                     /db_xref="CDD:400500"
     Region          <204..322
                     /region_name="RNA_pol_Rpc82"
                     /note="RNA polymerase III subunit RPC82; pfam05645"
                     /db_xref="CDD:398977"
     CDS             1..508
                     /gene="Polr3C"
                     /coded_by="XM_034339924.2:1..1527"
                     /db_xref="GeneID:117611740"
ORIGIN      
        1 mlyvinvnms lmcrklccsi lyehfgqivq cvgedllkyg tkplglirst tklplskike
       61 alcvlikygf vthqknekav eyslscekvl mvlryprymf fvkticgdes emmleevlkh
      121 gyitasdliv ktykrieqlp sdlqpsvpvl kdkfellvkt qflirslcee avqetaflkt
      181 dftvpdlnla aiarkiegnd nvdagddkiy wnvnfdrftq dlrdqiivsa mqkrldenag
      241 elmrqllflm ylrtaswadt snpiplteik davkkqnyps ltqyidqylr lieedssqfm
      301 krvgdsgggq ysvnmkeaft qlawttleni vmerfgskaa rifrlvkdrk yieqeriqql
      361 amipakeakh ltytllqeny lqmqelkkpg vsaapiktff lfhidlnmvv rmeiehcyha
      421 lyntiqrrey evsrnkrmid kqlrmqtlts nlkehgatee qlaeiaelmt psekqqlgkv
      481 qntikklgit elqiddtlfl ltmylryh
//
LOCUS       XP_034195819             196 aa            linear   INV 03-SEP-2025
DEFINITION  deoxycytidylate deaminase-like [Osmia lignaria lignaria].
ACCESSION   XP_034195819
VERSION     XP_034195819.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339928.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="deoxycytidylate deaminase-like"
                     /calculated_mol_wt=22204
     Region          19..170
                     /region_name="ComEB"
                     /note="Deoxycytidylate deaminase [Nucleotide transport and
                     metabolism]; COG2131"
                     /db_xref="CDD:441734"
     CDS             1..196
                     /gene="LOC117611741"
                     /coded_by="XM_034339928.2:101..691"
                     /db_xref="GeneID:117611741"
ORIGIN      
        1 maqndifvet nskcdinnkn kkyiewdeyf mgiaflaakr skdpktqvga civndekril
       61 gvgyngmptg csddefpwgk dyndklqdks yyvchaeina vlnkncsdvk ncsiyvalfp
      121 cnecakvliq sgiklvvfms dkqahkketv aakrmfdaag vkyrqyipkn qqividfsev
      181 dkndkiqels krlesi
//
LOCUS       XP_034195808             875 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor Sox102F isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195808
VERSION     XP_034195808.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339917.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..875
                     /product="transcription factor Sox102F isoform X1"
                     /calculated_mol_wt=95168
     Region          676..753
                     /region_name="HMG-box_EGL13-like"
                     /note="high mobility group (HMG)-box found in
                     Caenorhabditis elegans protein egg laying defective 13
                     (EGL-13) and similar proteins; cd22042"
                     /db_xref="CDD:438848"
     Site            order(677,679..680,682..683,685..686,690,693,697,704..706,
                     709..710,713,716..717,735,745,747..750)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438848"
     Region          <781..857
                     /region_name="PHA03185"
                     /note="UL14 tegument protein; Provisional"
                     /db_xref="CDD:177553"
     CDS             1..875
                     /gene="Sox102F"
                     /coded_by="XM_034339917.2:1441..4068"
                     /db_xref="GeneID:117611738"
ORIGIN      
        1 mrvyvfrnar krangrsrti fhptipthii rmdliglrms skrkspptkl seggggtgtv
       61 iganeeeedt tssvtggpgg evavgeegpt tpvdieecyp hqrggdcess gcsspattse
      121 pdlrdspsps snslrpskgq rivlpgqetl apyhyphhhh hhhhhhhhhh tntssssggv
      181 vepasssecg spptpltvdp yypnhphhnn nntvtqnnnn snggnattas tkrsmddvlk
      241 rltckmnses lslqddtsnn rrtspppsst ptghnahcgg vasvpnsvpp sppasgrmqn
      301 tegqvqqdga salhrvlcae sfaekertls emilqlqllr eqllqqqdqt ksypvhmsvd
      361 sqkqieiqrl qtehlkrqqe himqhniqel qaqmtksqln msgpqslmfl pfleqlrglp
      421 vqspmppppa tsttttnkhi nsianmissh regpswatah laqmttqmek easptpissa
      481 vaptapplqd ldaplnltkp kssssgatas ssspgsdshs tgagssgqqe qplaatapkl
      541 fppglpmprn ylpslpyagl pphlstlssp mgkvmskdea gsaggaaaaa avaavekhfa
      601 mhgiyalpps agamppspqt qrpmkhsasr deaaqeeqdy lstshmwrep gykvpedite
      661 kakmvrqqkr egenkphikr pmnafmvwak derrkilkac pdmhnsnisk ilgarwkams
      721 nsekqpyyee qsrlsklhme khpdyryrpr pkrtcivdgk kmriseyksl mrqrrqemrq
      781 lwcrdsgqem sflspvgsdl ssqhhpgtga gpsarpsvsp patmlnggaa gpssdhpsfy
      841 ypqdslspsd mmdfspdnsg siggydaspr hhded
//
LOCUS       XP_034195807             875 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor Sox102F isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195807
VERSION     XP_034195807.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339916.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..875
                     /product="transcription factor Sox102F isoform X1"
                     /calculated_mol_wt=95168
     Region          676..753
                     /region_name="HMG-box_EGL13-like"
                     /note="high mobility group (HMG)-box found in
                     Caenorhabditis elegans protein egg laying defective 13
                     (EGL-13) and similar proteins; cd22042"
                     /db_xref="CDD:438848"
     Site            order(677,679..680,682..683,685..686,690,693,697,704..706,
                     709..710,713,716..717,735,745,747..750)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438848"
     Region          <781..857
                     /region_name="PHA03185"
                     /note="UL14 tegument protein; Provisional"
                     /db_xref="CDD:177553"
     CDS             1..875
                     /gene="Sox102F"
                     /coded_by="XM_034339916.2:1050..3677"
                     /db_xref="GeneID:117611738"
ORIGIN      
        1 mrvyvfrnar krangrsrti fhptipthii rmdliglrms skrkspptkl seggggtgtv
       61 iganeeeedt tssvtggpgg evavgeegpt tpvdieecyp hqrggdcess gcsspattse
      121 pdlrdspsps snslrpskgq rivlpgqetl apyhyphhhh hhhhhhhhhh tntssssggv
      181 vepasssecg spptpltvdp yypnhphhnn nntvtqnnnn snggnattas tkrsmddvlk
      241 rltckmnses lslqddtsnn rrtspppsst ptghnahcgg vasvpnsvpp sppasgrmqn
      301 tegqvqqdga salhrvlcae sfaekertls emilqlqllr eqllqqqdqt ksypvhmsvd
      361 sqkqieiqrl qtehlkrqqe himqhniqel qaqmtksqln msgpqslmfl pfleqlrglp
      421 vqspmppppa tsttttnkhi nsianmissh regpswatah laqmttqmek easptpissa
      481 vaptapplqd ldaplnltkp kssssgatas ssspgsdshs tgagssgqqe qplaatapkl
      541 fppglpmprn ylpslpyagl pphlstlssp mgkvmskdea gsaggaaaaa avaavekhfa
      601 mhgiyalpps agamppspqt qrpmkhsasr deaaqeeqdy lstshmwrep gykvpedite
      661 kakmvrqqkr egenkphikr pmnafmvwak derrkilkac pdmhnsnisk ilgarwkams
      721 nsekqpyyee qsrlsklhme khpdyryrpr pkrtcivdgk kmriseyksl mrqrrqemrq
      781 lwcrdsgqem sflspvgsdl ssqhhpgtga gpsarpsvsp patmlnggaa gpssdhpsfy
      841 ypqdslspsd mmdfspdnsg siggydaspr hhded
//
LOCUS       XP_034195809             845 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor Sox102F isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195809
VERSION     XP_034195809.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339918.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..845
                     /product="transcription factor Sox102F isoform X2"
                     /calculated_mol_wt=91536
     Region          646..723
                     /region_name="HMG-box_EGL13-like"
                     /note="high mobility group (HMG)-box found in
                     Caenorhabditis elegans protein egg laying defective 13
                     (EGL-13) and similar proteins; cd22042"
                     /db_xref="CDD:438848"
     Site            order(647,649..650,652..653,655..656,660,663,667,674..676,
                     679..680,683,686..687,705,715,717..720)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438848"
     Region          <751..827
                     /region_name="PHA03185"
                     /note="UL14 tegument protein; Provisional"
                     /db_xref="CDD:177553"
     CDS             1..845
                     /gene="Sox102F"
                     /coded_by="XM_034339918.2:1420..3957"
                     /db_xref="GeneID:117611738"
ORIGIN      
        1 mstlgrlrms skrkspptkl seggggtgtv iganeeeedt tssvtggpgg evavgeegpt
       61 tpvdieecyp hqrggdcess gcsspattse pdlrdspsps snslrpskgq rivlpgqetl
      121 apyhyphhhh hhhhhhhhhh tntssssggv vepasssecg spptpltvdp yypnhphhnn
      181 nntvtqnnnn snggnattas tkrsmddvlk rltckmnses lslqddtsnn rrtspppsst
      241 ptghnahcgg vasvpnsvpp sppasgrmqn tegqvqqdga salhrvlcae sfaekertls
      301 emilqlqllr eqllqqqdqt ksypvhmsvd sqkqieiqrl qtehlkrqqe himqhniqel
      361 qaqmtksqln msgpqslmfl pfleqlrglp vqspmppppa tsttttnkhi nsianmissh
      421 regpswatah laqmttqmek easptpissa vaptapplqd ldaplnltkp kssssgatas
      481 ssspgsdshs tgagssgqqe qplaatapkl fppglpmprn ylpslpyagl pphlstlssp
      541 mgkvmskdea gsaggaaaaa avaavekhfa mhgiyalpps agamppspqt qrpmkhsasr
      601 deaaqeeqdy lstshmwrep gykvpedite kakmvrqqkr egenkphikr pmnafmvwak
      661 derrkilkac pdmhnsnisk ilgarwkams nsekqpyyee qsrlsklhme khpdyryrpr
      721 pkrtcivdgk kmriseyksl mrqrrqemrq lwcrdsgqem sflspvgsdl ssqhhpgtga
      781 gpsarpsvsp patmlnggaa gpssdhpsfy ypqdslspsd mmdfspdnsg siggydaspr
      841 hhded
//
LOCUS       XP_034195810             837 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor Sox102F isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195810
VERSION     XP_034195810.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..837
                     /product="transcription factor Sox102F isoform X3"
                     /calculated_mol_wt=90621
     Region          638..715
                     /region_name="HMG-box_EGL13-like"
                     /note="high mobility group (HMG)-box found in
                     Caenorhabditis elegans protein egg laying defective 13
                     (EGL-13) and similar proteins; cd22042"
                     /db_xref="CDD:438848"
     Site            order(639,641..642,644..645,647..648,652,655,659,666..668,
                     671..672,675,678..679,697,707,709..712)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438848"
     Region          <743..819
                     /region_name="PHA03185"
                     /note="UL14 tegument protein; Provisional"
                     /db_xref="CDD:177553"
     CDS             1..837
                     /gene="Sox102F"
                     /coded_by="XM_034339919.2:1842..4355"
                     /db_xref="GeneID:117611738"
ORIGIN      
        1 msskrksppt klseggggtg tviganeeee dttssvtggp ggevavgeeg pttpvdieec
       61 yphqrggdce ssgcsspatt sepdlrdsps pssnslrpsk gqrivlpgqe tlapyhyphh
      121 hhhhhhhhhh hhtntssssg gvvepassse cgspptpltv dpyypnhphh nnnntvtqnn
      181 nnsnggnatt astkrsmddv lkrltckmns eslslqddts nnrrtsppps stptghnahc
      241 ggvasvpnsv ppsppasgrm qntegqvqqd gasalhrvlc aesfaekert lsemilqlql
      301 lreqllqqqd qtksypvhms vdsqkqieiq rlqtehlkrq qehimqhniq elqaqmtksq
      361 lnmsgpqslm flpfleqlrg lpvqspmppp patsttttnk hinsianmis shregpswat
      421 ahlaqmttqm ekeasptpis savaptappl qdldaplnlt kpkssssgat asssspgsds
      481 hstgagssgq qeqplaatap klfppglpmp rnylpslpya glpphlstls spmgkvmskd
      541 eagsaggaaa aaavaavekh famhgiyalp psagamppsp qtqrpmkhsa srdeaaqeeq
      601 dylstshmwr epgykvpedi tekakmvrqq kregenkphi krpmnafmvw akderrkilk
      661 acpdmhnsni skilgarwka msnsekqpyy eeqsrlsklh mekhpdyryr prpkrtcivd
      721 gkkmriseyk slmrqrrqem rqlwcrdsgq emsflspvgs dlssqhhpgt gagpsarpsv
      781 sppatmlngg aagpssdhps fyypqdslsp sdmmdfspdn sgsiggydas prhhded
//
LOCUS       XP_034195811             837 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor Sox102F isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034195811
VERSION     XP_034195811.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339920.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..837
                     /product="transcription factor Sox102F isoform X3"
                     /calculated_mol_wt=90621
     Region          638..715
                     /region_name="HMG-box_EGL13-like"
                     /note="high mobility group (HMG)-box found in
                     Caenorhabditis elegans protein egg laying defective 13
                     (EGL-13) and similar proteins; cd22042"
                     /db_xref="CDD:438848"
     Site            order(639,641..642,644..645,647..648,652,655,659,666..668,
                     671..672,675,678..679,697,707,709..712)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438848"
     Region          <743..819
                     /region_name="PHA03185"
                     /note="UL14 tegument protein; Provisional"
                     /db_xref="CDD:177553"
     CDS             1..837
                     /gene="Sox102F"
                     /coded_by="XM_034339920.2:461..2974"
                     /db_xref="GeneID:117611738"
ORIGIN      
        1 msskrksppt klseggggtg tviganeeee dttssvtggp ggevavgeeg pttpvdieec
       61 yphqrggdce ssgcsspatt sepdlrdsps pssnslrpsk gqrivlpgqe tlapyhyphh
      121 hhhhhhhhhh hhtntssssg gvvepassse cgspptpltv dpyypnhphh nnnntvtqnn
      181 nnsnggnatt astkrsmddv lkrltckmns eslslqddts nnrrtsppps stptghnahc
      241 ggvasvpnsv ppsppasgrm qntegqvqqd gasalhrvlc aesfaekert lsemilqlql
      301 lreqllqqqd qtksypvhms vdsqkqieiq rlqtehlkrq qehimqhniq elqaqmtksq
      361 lnmsgpqslm flpfleqlrg lpvqspmppp patsttttnk hinsianmis shregpswat
      421 ahlaqmttqm ekeasptpis savaptappl qdldaplnlt kpkssssgat asssspgsds
      481 hstgagssgq qeqplaatap klfppglpmp rnylpslpya glpphlstls spmgkvmskd
      541 eagsaggaaa aaavaavekh famhgiyalp psagamppsp qtqrpmkhsa srdeaaqeeq
      601 dylstshmwr epgykvpedi tekakmvrqq kregenkphi krpmnafmvw akderrkilk
      661 acpdmhnsni skilgarwka msnsekqpyy eeqsrlsklh mekhpdyryr prpkrtcivd
      721 gkkmriseyk slmrqrrqem rqlwcrdsgq emsflspvgs dlssqhhpgt gagpsarpsv
      781 sppatmlngg aagpssdhps fyypqdslsp sdmmdfspdn sgsiggydas prhhded
//
LOCUS       XP_034195812             781 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor Sox102F isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034195812
VERSION     XP_034195812.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339921.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..781
                     /product="transcription factor Sox102F isoform X4"
                     /calculated_mol_wt=84443
     Region          582..659
                     /region_name="HMG-box_EGL13-like"
                     /note="high mobility group (HMG)-box found in
                     Caenorhabditis elegans protein egg laying defective 13
                     (EGL-13) and similar proteins; cd22042"
                     /db_xref="CDD:438848"
     Site            order(583,585..586,588..589,591..592,596,599,603,610..612,
                     615..616,619,622..623,641,651,653..656)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:438848"
     Region          <687..763
                     /region_name="PHA03185"
                     /note="UL14 tegument protein; Provisional"
                     /db_xref="CDD:177553"
     CDS             1..781
                     /gene="Sox102F"
                     /coded_by="XM_034339921.2:1443..3788"
                     /db_xref="GeneID:117611738"
ORIGIN      
        1 mrvyvfrnar krangrsrti fhptipthii rmdliglrms skrkspptkl seggggtgtv
       61 iganeeeedt tssvtggpgg evavgeegpt tpvdieecyp hqrggdcess gcsspattse
      121 pdlrdspsps snslrpskgq rivlpgqetl apyhyphhhh hhhhhhhhhh tntssssggv
      181 vepasssecg spptpltvdp yypnhphhnn nntvtqnnnn snggnattas tkrsmddvlk
      241 rltckmnses lslqddtsnn rrtspppsst ptghnahcgg vasvpnsvpp sppasgrmqn
      301 tegqvqqdga salhrvlcae sfaekertls emilqlqllr eqllqqqdqt kmisshregp
      361 swatahlaqm ttqmekeasp tpissavapt applqdldap lnltkpksss sgatassssp
      421 gsdshstgag ssgqqeqpla atapklfppg lpmprnylps lpyaglpphl stlsspmgkv
      481 mskdeagsag gaaaaaavaa vekhfamhgi yalppsagam ppspqtqrpm khsasrdeaa
      541 qeeqdylsts hmwrepgykv peditekakm vrqqkregen kphikrpmna fmvwakderr
      601 kilkacpdmh nsniskilga rwkamsnsek qpyyeeqsrl sklhmekhpd yryrprpkrt
      661 civdgkkmri seykslmrqr rqemrqlwcr dsgqemsfls pvgsdlssqh hpgtgagpsa
      721 rpsvsppatm lnggaagpss dhpsfyypqd slspsdmmdf spdnsgsigg ydasprhhde
      781 d
//
LOCUS       XP_034195841             526 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195841
VERSION     XP_034195841.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339950.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..526
                     /product="ubiquilin isoform X1"
                     /calculated_mol_wt=57987
     Region          9..81
                     /region_name="Ubl_PLICs"
                     /note="ubiquitin-like (Ubl) domain found in eukaryotic
                     protein linking integrin-associated protein (IAP, also
                     known as CD47) with cytoskeleton (PLIC) proteins; cd01808"
                     /db_xref="CDD:340506"
     Site            order(16,53..58,74..77,79,81)
                     /site_type="other"
                     /note="Pru interaction site [polypeptide binding]"
                     /db_xref="CDD:340506"
     Site            order(46,49,53,55..58,60,77,79..81)
                     /site_type="other"
                     /note="putative S5a binding site"
                     /db_xref="CDD:340506"
     Region          480..519
                     /region_name="UBA_PLICs"
                     /note="UBA domain of eukaryotic protein linking
                     integrin-associated protein (IAP, also known as CD47) with
                     cytoskeleton (PLIC) proteins; cd14399"
                     /db_xref="CDD:270582"
     Site            order(490..495,511,514..516,518..519)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270582"
     CDS             1..526
                     /gene="Ubqn"
                     /coded_by="XM_034339950.2:459..2039"
                     /db_xref="GeneID:117611750"
ORIGIN      
        1 maegedtrkk itvnvqtske kqsveieeda sikdfkeavs kkfnaqteql clifagkimk
       61 dhetlsthnv kdgltvhlvi kaprtpsnqs qdsslsqrlq adisaspfgl gslgglpgle
      121 nlglgsanfi dmhqrmqrel lsnpetmrqv ldnplvqslm ndpenmrnlv tanpqmqelm
      181 qrnpeishml nnpellrqtm elarnpamlq elmrshdral snlesipggh salrrmyrdi
      241 qepmfaaaan ernpfaalvd nsnnqdgqan pqqgqenrdp lpnpwaqnqs dstaqpqgrg
      301 lldspgmqsl aaqmmenpql mrnmlnapyt rsmleamaad pnmasrmist npffranpqm
      361 qeqlrtmmpa fiqqmqnpqi qnvvtnpdal aaimqvqqgi eqlrtiapef venmglsmpa
      421 atttttppna satntstptt qstdtnqqda fsqfmarmvs amalnqgvdg qsaalppeer
      481 yrvqleqlta mgfvnrdanl qaliatfgdi naaierllsn gqvsms
//
LOCUS       XP_076546821             526 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546821
VERSION     XP_076546821.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690706.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..526
                     /product="ubiquilin isoform X1"
                     /calculated_mol_wt=57987
     Region          9..81
                     /region_name="Ubl_PLICs"
                     /note="ubiquitin-like (Ubl) domain found in eukaryotic
                     protein linking integrin-associated protein (IAP, also
                     known as CD47) with cytoskeleton (PLIC) proteins; cd01808"
                     /db_xref="CDD:340506"
     Site            order(16,53..58,74..77,79,81)
                     /site_type="other"
                     /note="Pru interaction site [polypeptide binding]"
                     /db_xref="CDD:340506"
     Site            order(46,49,53,55..58,60,77,79..81)
                     /site_type="other"
                     /note="putative S5a binding site"
                     /db_xref="CDD:340506"
     Region          480..519
                     /region_name="UBA_PLICs"
                     /note="UBA domain of eukaryotic protein linking
                     integrin-associated protein (IAP, also known as CD47) with
                     cytoskeleton (PLIC) proteins; cd14399"
                     /db_xref="CDD:270582"
     Site            order(490..495,511,514..516,518..519)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270582"
     CDS             1..526
                     /gene="Ubqn"
                     /coded_by="XM_076690706.1:425..2005"
                     /db_xref="GeneID:117611750"
ORIGIN      
        1 maegedtrkk itvnvqtske kqsveieeda sikdfkeavs kkfnaqteql clifagkimk
       61 dhetlsthnv kdgltvhlvi kaprtpsnqs qdsslsqrlq adisaspfgl gslgglpgle
      121 nlglgsanfi dmhqrmqrel lsnpetmrqv ldnplvqslm ndpenmrnlv tanpqmqelm
      181 qrnpeishml nnpellrqtm elarnpamlq elmrshdral snlesipggh salrrmyrdi
      241 qepmfaaaan ernpfaalvd nsnnqdgqan pqqgqenrdp lpnpwaqnqs dstaqpqgrg
      301 lldspgmqsl aaqmmenpql mrnmlnapyt rsmleamaad pnmasrmist npffranpqm
      361 qeqlrtmmpa fiqqmqnpqi qnvvtnpdal aaimqvqqgi eqlrtiapef venmglsmpa
      421 atttttppna satntstptt qstdtnqqda fsqfmarmvs amalnqgvdg qsaalppeer
      481 yrvqleqlta mgfvnrdanl qaliatfgdi naaierllsn gqvsms
//
LOCUS       XP_076546822             526 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquilin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546822
VERSION     XP_076546822.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690707.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..526
                     /product="ubiquilin isoform X1"
                     /calculated_mol_wt=57987
     Region          9..81
                     /region_name="Ubl_PLICs"
                     /note="ubiquitin-like (Ubl) domain found in eukaryotic
                     protein linking integrin-associated protein (IAP, also
                     known as CD47) with cytoskeleton (PLIC) proteins; cd01808"
                     /db_xref="CDD:340506"
     Site            order(16,53..58,74..77,79,81)
                     /site_type="other"
                     /note="Pru interaction site [polypeptide binding]"
                     /db_xref="CDD:340506"
     Site            order(46,49,53,55..58,60,77,79..81)
                     /site_type="other"
                     /note="putative S5a binding site"
                     /db_xref="CDD:340506"
     Region          480..519
                     /region_name="UBA_PLICs"
                     /note="UBA domain of eukaryotic protein linking
                     integrin-associated protein (IAP, also known as CD47) with
                     cytoskeleton (PLIC) proteins; cd14399"
                     /db_xref="CDD:270582"
     Site            order(490..495,511,514..516,518..519)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270582"
     CDS             1..526
                     /gene="Ubqn"
                     /coded_by="XM_076690707.1:228..1808"
                     /db_xref="GeneID:117611750"
ORIGIN      
        1 maegedtrkk itvnvqtske kqsveieeda sikdfkeavs kkfnaqteql clifagkimk
       61 dhetlsthnv kdgltvhlvi kaprtpsnqs qdsslsqrlq adisaspfgl gslgglpgle
      121 nlglgsanfi dmhqrmqrel lsnpetmrqv ldnplvqslm ndpenmrnlv tanpqmqelm
      181 qrnpeishml nnpellrqtm elarnpamlq elmrshdral snlesipggh salrrmyrdi
      241 qepmfaaaan ernpfaalvd nsnnqdgqan pqqgqenrdp lpnpwaqnqs dstaqpqgrg
      301 lldspgmqsl aaqmmenpql mrnmlnapyt rsmleamaad pnmasrmist npffranpqm
      361 qeqlrtmmpa fiqqmqnpqi qnvvtnpdal aaimqvqqgi eqlrtiapef venmglsmpa
      421 atttttppna satntstptt qstdtnqqda fsqfmarmvs amalnqgvdg qsaalppeer
      481 yrvqleqlta mgfvnrdanl qaliatfgdi naaierllsn gqvsms
//
LOCUS       XP_034195843             516 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquilin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195843
VERSION     XP_034195843.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339952.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="ubiquilin isoform X2"
                     /calculated_mol_wt=57028
     Region          9..81
                     /region_name="Ubl_PLICs"
                     /note="ubiquitin-like (Ubl) domain found in eukaryotic
                     protein linking integrin-associated protein (IAP, also
                     known as CD47) with cytoskeleton (PLIC) proteins; cd01808"
                     /db_xref="CDD:340506"
     Site            order(16,53..58,74..77,79,81)
                     /site_type="other"
                     /note="Pru interaction site [polypeptide binding]"
                     /db_xref="CDD:340506"
     Site            order(46,49,53,55..58,60,77,79..81)
                     /site_type="other"
                     /note="putative S5a binding site"
                     /db_xref="CDD:340506"
     Region          163..>204
                     /region_name="STI1"
                     /note="STI1 domain; cl17974"
                     /db_xref="CDD:450205"
     Region          470..509
                     /region_name="UBA_PLICs"
                     /note="UBA domain of eukaryotic protein linking
                     integrin-associated protein (IAP, also known as CD47) with
                     cytoskeleton (PLIC) proteins; cd14399"
                     /db_xref="CDD:270582"
     Site            order(480..485,501,504..506,508..509)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270582"
     CDS             1..516
                     /gene="Ubqn"
                     /coded_by="XM_034339952.2:461..2011"
                     /db_xref="GeneID:117611750"
ORIGIN      
        1 maegedtrkk itvnvqtske kqsveieeda sikdfkeavs kkfnaqteql clifagkimk
       61 dhetlsthnv kdgltvhlvi kaprtpsnqs qdsslsqrlq gslgglpgle nlglgsanfi
      121 dmhqrmqrel lsnpetmrqv ldnplvqslm ndpenmrnlv tanpqmqelm qrnpeishml
      181 nnpellrqtm elarnpamlq elmrshdral snlesipggh salrrmyrdi qepmfaaaan
      241 ernpfaalvd nsnnqdgqan pqqgqenrdp lpnpwaqnqs dstaqpqgrg lldspgmqsl
      301 aaqmmenpql mrnmlnapyt rsmleamaad pnmasrmist npffranpqm qeqlrtmmpa
      361 fiqqmqnpqi qnvvtnpdal aaimqvqqgi eqlrtiapef venmglsmpa atttttppna
      421 satntstptt qstdtnqqda fsqfmarmvs amalnqgvdg qsaalppeer yrvqleqlta
      481 mgfvnrdanl qaliatfgdi naaierllsn gqvsms
//
LOCUS       XP_034193453             473 aa            linear   INV 03-SEP-2025
DEFINITION  katanin p60 ATPase-containing subunit A-like 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034193453
VERSION     XP_034193453.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337562.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193453.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="katanin p60 ATPase-containing subunit A-like 2"
                     /calculated_mol_wt=53641
     Region          25..>335
                     /region_name="McrB"
                     /note="5-methylcytosine-specific restriction endonuclease
                     McrBC, GTP-binding regulatory subunit McrB [Defense
                     mechanisms]; COG1401"
                     /db_xref="CDD:441011"
     Region          <202..418
                     /region_name="RPT1"
                     /note="ATP-dependent 26S proteasome regulatory subunit
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG1222"
                     /db_xref="CDD:440835"
     CDS             1..473
                     /gene="LOC117610314"
                     /coded_by="XM_034337562.2:72..1493"
                     /db_xref="GeneID:117610314"
ORIGIN      
        1 mnlglsmdgs lnqinhklre kekkrteerr rsipyliaqy lkqhgltdsy gtlfseaqlp
       61 tdiqiadnid lemilmeyds yyhlkfnkyp ilyktvests stnpikkikt viksnkndta
      121 kedvahqtnf tpendisfgl tvsplfpken vnennnltkd vpnnrptksk ilscieklyp
      181 sdselrkiae disteivlgn lnvswddvvg ldkckaaike aivyplkypi ffsdkfspwk
      241 gillygppgt gktmlakaaa tecnctfinv tasllvskwr gdsekyirvl fdlaynqspa
      301 iifideidwi stnntndsls epakrfrael ltrldgllsp egsnvmllaa tnapwnidaa
      361 llrrlekqiy vtlpdehsrl nlfssyvspn imeakrnylv evtnyysaae iklickeawi
      421 iqsaptwnrl enneitvtnl qfnitslssl vhaikniqgt dknipkyddw iks
//
LOCUS       XP_076546823             462 aa            linear   INV 03-SEP-2025
DEFINITION  G-patch domain and KOW motifs-containing protein-like isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_076546823
VERSION     XP_076546823.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690708.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="G-patch domain and KOW motifs-containing
                     protein-like isoform X1"
                     /calculated_mol_wt=52118
     Region          166..224
                     /region_name="G-patch_2"
                     /note="G-patch domain; pfam12656"
                     /db_xref="CDD:432700"
     Region          244..300
                     /region_name="KOW_GPKOW_A"
                     /note="KOW motif of the 'G-patch domain and KOW
                     motifs-containing protein' (GPKOW) repeat A; cd13152"
                     /db_xref="CDD:240516"
     CDS             1..462
                     /gene="LOC143305789"
                     /coded_by="XM_076690708.1:205..1593"
                     /db_xref="GeneID:143305789"
ORIGIN      
        1 maeegkkisf gfvksikkpv lkstvpqeek kvdyieclde kgikvigeek kkdeplvipl
       61 lgsktwhdri inkidadife pktkeeptdd sakqegkvdv pngsllpqis vkeeviende
      121 nkavtleeqa akeiiedlns kekeqqsetk tltlsltddp slrgteqstl edydkipvda
      181 fglamlrgmg wqpgkgigkn ekvvsavipe lrpkgmglga dkvtlqkknt dskkqeeelk
      241 iekgtfvkii agkqsnnygq iegfdedagr liiklalggn iisvnefmvq pvtkseyskn
      301 skvlnvkkye eykdkastel khktvekkks assvssggse dddksnvgdk kkediscnrn
      361 kydkkskrrk rksesdsgdd dsdygkrrrr grsassnsds yeskkskksk khkrnspers
      421 skkhkkkdke rektkdrksr dftdkrkhkk rgrsrsrsfs kr
//
LOCUS       XP_076546824             311 aa            linear   INV 03-SEP-2025
DEFINITION  G-patch domain and KOW motifs-containing protein-like isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_076546824
VERSION     XP_076546824.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690709.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="G-patch domain and KOW motifs-containing
                     protein-like isoform X2"
                     /calculated_mol_wt=34437
     Region          166..224
                     /region_name="G-patch_2"
                     /note="G-patch domain; pfam12656"
                     /db_xref="CDD:432700"
     Region          244..300
                     /region_name="KOW_GPKOW_A"
                     /note="KOW motif of the 'G-patch domain and KOW
                     motifs-containing protein' (GPKOW) repeat A; cd13152"
                     /db_xref="CDD:240516"
     CDS             1..311
                     /gene="LOC143305789"
                     /coded_by="XM_076690709.1:205..1140"
                     /db_xref="GeneID:143305789"
ORIGIN      
        1 maeegkkisf gfvksikkpv lkstvpqeek kvdyieclde kgikvigeek kkdeplvipl
       61 lgsktwhdri inkidadife pktkeeptdd sakqegkvdv pngsllpqis vkeeviende
      121 nkavtleeqa akeiiedlns kekeqqsetk tltlsltddp slrgteqstl edydkipvda
      181 fglamlrgmg wqpgkgigkn ekvvsavipe lrpkgmglga dkvtlqkknt dskkqeeelk
      241 iekgtfvkii agkqsnnygq iegfdedagr liiklalggn iisvnefmvq pvtkseyskn
      301 skvltlsilw r
//
LOCUS       XP_034193461             255 aa            linear   INV 03-SEP-2025
DEFINITION  survival motor neuron isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193461
VERSION     XP_034193461.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337570.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193461.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..255
                     /product="survival motor neuron isoform X1"
                     /calculated_mol_wt=28159
     Region          22..246
                     /region_name="SMN"
                     /note="Survival motor neuron protein (SMN); pfam06003"
                     /db_xref="CDD:428716"
     CDS             1..255
                     /gene="Smn"
                     /coded_by="XM_034337570.2:120..887"
                     /db_xref="GeneID:117610323"
ORIGIN      
        1 maddnvlfvr gngngtnags edvwddsali kaydkavnla keevvkrmgm dvqnsqskqk
       61 sqnvkqprht nkplkkwivg apcravysed gevyeaiisk ihensgictv kfvgygntek
      121 vslssllese glqsqiaqqk naiaekfsgg ntetdeinfc tnanskkyng ekmdcesedt
      181 ktykhhfmsg rsfnstadmm ppapplppql maklpdndad alssmlmswy isgfhtgyyh
      241 glkqaknnqe krknc
//
LOCUS       XP_034193471             229 aa            linear   INV 03-SEP-2025
DEFINITION  survival motor neuron isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034193471
VERSION     XP_034193471.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337580.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193471.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..229
                     /product="survival motor neuron isoform X2"
                     /calculated_mol_wt=25222
     Region          22..>172
                     /region_name="SMN"
                     /note="Survival motor neuron protein (SMN); pfam06003"
                     /db_xref="CDD:428716"
     CDS             1..229
                     /gene="Smn"
                     /coded_by="XM_034337580.2:120..809"
                     /db_xref="GeneID:117610323"
ORIGIN      
        1 maddnvlfvr gngngtnags edvwddsali kaydkavnla keevvkrmgm dvqnsqskqk
       61 sqnvkqprht nkplkkwivg apcravysed gevyeaiisk ihensgictv kfvgygntek
      121 vslssllese glqsqiaqqk naiaekfsgg ntetdeinfc tnanskkyng ekmdcesedt
      181 ktykhhfmsg rsfnstadmm ppapplppql maknilgcqi mmqmhfqvc
//
LOCUS       XP_034193485             161 aa            linear   INV 03-SEP-2025
DEFINITION  adrenodoxin-like protein 2, mitochondrial Ferredoxin 2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034193485
VERSION     XP_034193485.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337594.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..161
                     /product="adrenodoxin-like protein 2, mitochondrial
                     Ferredoxin 2"
                     /calculated_mol_wt=17480
     Region          51..154
                     /region_name="PLN02593"
                     /note="adrenodoxin-like ferredoxin protein"
                     /db_xref="CDD:178203"
     Site            order(84..85,90,93,96..99,136..137)
                     /site_type="active"
                     /note="catalytic loop [active]"
                     /db_xref="CDD:238126"
     Site            order(90,96,99,137)
                     /site_type="other"
                     /note="iron binding site [ion binding]"
                     /db_xref="CDD:238126"
     CDS             1..161
                     /gene="Fdx2"
                     /coded_by="XM_034337594.2:56..541"
                     /db_xref="GeneID:117610335"
ORIGIN      
        1 malvnnfqrf srsilgvvsn yskctsnttl pflqairkts ttqplsekae vsitfvkadg
       61 ekikakgkig dtildivvnn eidldgfgac egtltcstch lifpkevyda lpdkptdeel
      121 dmldlayelt dtsrlgcqiv mtksldgiev rvpstindar a
//
LOCUS       XP_034193497             136 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610344 [Osmia lignaria lignaria].
ACCESSION   XP_034193497
VERSION     XP_034193497.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337606.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="uncharacterized protein LOC117610344"
                     /calculated_mol_wt=15083
     CDS             1..136
                     /gene="LOC117610344"
                     /coded_by="XM_034337606.2:304..714"
                     /db_xref="GeneID:117610344"
ORIGIN      
        1 mkrslslysk ilgwtvcliv vllfflpsrt aaleenctdf qgaivrhgll yvpgpavcsl
       61 cvcyhsepmw cqaifctppy kckkfrvger ccefeclddt danwtgpdli iaagssrike
      121 qgtvwlfgli allkvl
//
LOCUS       XP_076546825             136 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610344 [Osmia lignaria lignaria].
ACCESSION   XP_076546825
VERSION     XP_076546825.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690710.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="uncharacterized protein LOC117610344"
                     /calculated_mol_wt=15083
     CDS             1..136
                     /gene="LOC117610344"
                     /coded_by="XM_076690710.1:290..700"
                     /db_xref="GeneID:117610344"
ORIGIN      
        1 mkrslslysk ilgwtvcliv vllfflpsrt aaleenctdf qgaivrhgll yvpgpavcsl
       61 cvcyhsepmw cqaifctppy kckkfrvger ccefeclddt danwtgpdli iaagssrike
      121 qgtvwlfgli allkvl
//
LOCUS       XP_076546827             289 aa            linear   INV 03-SEP-2025
DEFINITION  omega-amidase NIT2-like [Osmia lignaria lignaria].
ACCESSION   XP_076546827
VERSION     XP_076546827.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690712.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="omega-amidase NIT2-like"
                     /calculated_mol_wt=32531
     Region          17..278
                     /region_name="nit"
                     /note="Nit1, Nit 2, and related proteins, and the
                     Nit1-like domain of NitFhit (class 10 nitrilases);
                     cd07572"
                     /db_xref="CDD:143596"
     Site            order(55,108,125,129,141,166..167,169..170,191)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:143596"
     Site            order(55,125,166)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:143596"
     Site            order(126..127,147..149,167,170..174,176..177,199..200,
                     203..204,206..208,210..211,241,271..272,275..276,278)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143596"
     CDS             1..289
                     /gene="LOC117610349"
                     /coded_by="XM_076690712.1:74..943"
                     /db_xref="GeneID:117610349"
ORIGIN      
        1 mvivniakqv rtmstfrlal vqlqvdeikv knidrtvsfi sdakkqnadi ialpecfnsp
       61 ygiqhfpkya esipdgetsv alseaakens iyiiggtipe rdgnklyntc tiwgpngtli
      121 tkhrkvhlfd idipnkitfr esdslsagns ltmfevkgck igigicydir feemariyrn
      181 kgcqmliypa afnmttgpmh wsllqrsrsn dnqlyvacis parvpsagyv ayghtqltnp
      241 wgevlhdlet qesmvvadid lkvvnevraq iptffqrrtd lydtiwkkd
//
LOCUS       XP_034196331            1677 aa            linear   INV 03-SEP-2025
DEFINITION  carboxypeptidase D svr isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196331
VERSION     XP_034196331.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340440.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196331.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1677
                     /product="carboxypeptidase D svr isoform X1"
                     /calculated_mol_wt=189904
     Region          45..343
                     /region_name="M14_CPD_I"
                     /note="Peptidase M14 carboxypeptidase subfamily N/E-like;
                     Carboxypeptidase D, domain I subgroup; cd03868"
                     /db_xref="CDD:349440"
     Site            order(106,109,170,179..180,226..227,233,237,288,292,294,
                     314)
                     /site_type="active"
                     /db_xref="CDD:349440"
     Region          347..422
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cd11308"
                     /db_xref="CDD:200604"
     Region          458..757
                     /region_name="M14_CP_N-E_like"
                     /note="Peptidase M14 carboxypeptidase subfamily N/E-like;
                     cd03858"
                     /db_xref="CDD:349431"
     Site            order(519,522,579,588..589,627..628,634,638,702,706,708,
                     728)
                     /site_type="active"
                     /db_xref="CDD:349431"
     Region          761..839
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cd11308"
                     /db_xref="CDD:200604"
     Region          868..1111
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     Site            order(923,926,984,993..994,1025..1026,1083)
                     /site_type="active"
                     /db_xref="CDD:349427"
     Region          1116..1181
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cl21470"
                     /db_xref="CDD:473874"
     Region          1215..1474
                     /region_name="Peptidase_M14_like"
                     /note="M14 family of metallocarboxypeptidases and related
                     proteins; cl11393"
                     /db_xref="CDD:472171"
     Region          1480..1546
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cd11308"
                     /db_xref="CDD:200604"
     CDS             1..1677
                     /gene="svr"
                     /coded_by="XM_034340440.2:183..5216"
                     /db_xref="GeneID:117611932"
ORIGIN      
        1 mytnmvhvhr mynivlficl fvgsingfvi nsettieenf iiphythydk lkqlfsslve
       61 sypnlakvss igksvegrdl lvfeisenvr erklcepmvk yvanmhgdea vgrelliyla
      121 qyllhnygrd eritklvnnt diflmpsmnp dgfekseegk ceskkdfsgr enanhvdlnr
      181 dfpdqfnlrt nhlqkggsil dgrqnetiam mtwiatepfv lsgnlhggav vasypydsgi
      241 ssscciesks pddelfkyla htyadnhpqm ragnacpsdv fqggvtngay wyeviggmqd
      301 fnyarsnafe itfelsccky pnastmpehw rlnkepliky leqahigvkg vvrdtdgqpi
      361 etasiivdgi nhniytthrg eywrlllpgt yrihaeawgy qpsepvnvtv dldkpiilnf
      421 tlkqdtyndq gnlngseiee iirpidkygf fqdtkfehhn yvamekylkd lhsnypnitr
      481 lyrigesvkg rqlyvmeite npgkhnqnkp evkyignmhg nevvgreill lllkylcenf
      541 gsddrvtkil knvrlhilps mnpdgyeisk ggvygtrgra nakgvdlnrn fpdqyetndy
      601 nkeqepetka vmdwiasipf vlsanfhgga lvanypydne pqyasdaenl spddkvfkal
      661 alaysnahpr mhlgepcppl pnerlnfqnv leksfpngit ngaawysvsg gmqdynyvhs
      721 ndfeitvevg ctkfpnatel pkywlqnrep llrliemsrk gihgvvrssi gspiphakis
      781 vegikhdiya adggdywrll vpgkynvtas avgyepqtqs vtvpdtadig eertvdftlm
      841 rddpqhwssa ydfrlianlq nvylknaels arlsqleshq pnvaefqage slvsmaihsl
      901 kithnmggpe enkfhiglig glfasqpvgr emllrlathi lmgnqigdpp iqrilnnsvl
      961 hfipgvdpgf dnildiqecn pivydevgtr llqkdntnpd klnivasafk tmlsnekydv
     1021 iiilgsgtlk isytdnnmnm ykilaknyeq svhkgtcsfl nndvkevqny iqkeynipvi
     1081 sinlscckyp paesiptiwr enllplmqli rslttgiram vtdinnvplr navikvgtqs
     1141 yhvsknmayf killlpgeyl lriscedyie qsikvhvnne nifdthvkmi khrtkeielq
     1201 khdedrkpti vnqvltdlnn kysqlstlht iskteegiri mcleigienn ykrigrpsia
     1261 fvagisngvp vtsklllhfa tyllehygkd tritnyldkf tifiapdltq ntnhsqscsp
     1321 fiindlqfpi dntltpnanv iinwfkqint alavnlnigs qhieipfask ygkihgeiyq
     1381 tddddvlqdi alsytkynih mtstnskcnh dlrintngii hggmgiggkr kdslmdylyl
     1441 ntstlmiday vtccntddak niwennkasl lamveklneg vkgyvlnknn epienailsy
     1501 nksihhvrng lngaywlllq pgthvisasa pgyiqqnkif itpdvhdisy lifklgyndk
     1561 fmgmprivfi ilisticlgi itcivfiwan crssknvetk nwngyafsll rdgtsffddd
     1621 ekeieifrrp tngympttev tkpyfdddni ssseeasdle fiqpskewne eatkenr
//
LOCUS       XP_034196332             433 aa            linear   INV 03-SEP-2025
DEFINITION  carboxypeptidase D svr isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196332
VERSION     XP_034196332.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340441.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196332.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="carboxypeptidase D svr isoform X2"
                     /calculated_mol_wt=49105
     Region          45..343
                     /region_name="M14_CPD_I"
                     /note="Peptidase M14 carboxypeptidase subfamily N/E-like;
                     Carboxypeptidase D, domain I subgroup; cd03868"
                     /db_xref="CDD:349440"
     Site            order(106,109,170,179..180,226..227,233,237,288,292,294,
                     314)
                     /site_type="active"
                     /db_xref="CDD:349440"
     Region          347..422
                     /region_name="Peptidase_M14NE-CP-C_like"
                     /note="Peptidase associated domain: C-terminal domain of
                     M14 N/E carboxypeptidase; putative folding, regulation, or
                     interaction domain; cd11308"
                     /db_xref="CDD:200604"
     CDS             1..433
                     /gene="svr"
                     /coded_by="XM_034340441.2:144..1445"
                     /db_xref="GeneID:117611932"
ORIGIN      
        1 mytnmvhvhr mynivlficl fvgsingfvi nsettieenf iiphythydk lkqlfsslve
       61 sypnlakvss igksvegrdl lvfeisenvr erklcepmvk yvanmhgdea vgrelliyla
      121 qyllhnygrd eritklvnnt diflmpsmnp dgfekseegk ceskkdfsgr enanhvdlnr
      181 dfpdqfnlrt nhlqkggsil dgrqnetiam mtwiatepfv lsgnlhggav vasypydsgi
      241 ssscciesks pddelfkyla htyadnhpqm ragnacpsdv fqggvtngay wyeviggmqd
      301 fnyarsnafe itfelsccky pnastmpehw rlnkepliky leqahigvkg vvrdtdgqpi
      361 etasiivdgi nhniytthrg eywrlllpgt yrihaeawgy qpsepvnvtv dldkpiilnf
      421 tlkqdtyndq afi
//
LOCUS       XP_034196333            1292 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic FMR1-interacting protein Sra-1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196333
VERSION     XP_034196333.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340442.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1292
                     /product="cytoplasmic FMR1-interacting protein Sra-1"
                     /calculated_mol_wt=149818
     Region          386..1244
                     /region_name="FragX_IP"
                     /note="Cytoplasmic Fragile-X interacting family;
                     pfam05994"
                     /db_xref="CDD:461799"
     CDS             1..1292
                     /gene="Sra-1"
                     /coded_by="XM_034340442.2:378..4256"
                     /db_xref="GeneID:117611933"
ORIGIN      
        1 matdkvtlsd alsnvdvlde ftlpyeqpci eaqpcsivyq anfdtnfedr ngfvtgiaky
       61 ieeatvhasl nelleeglkh avmlytwrcc sraipqpksn eqpnrveiye ktvevlapev
      121 nkllnfmyfq rkaierfsge vkrlchhekr kdfvseayll tlgkfinmfa vldelknmks
      181 svkndystyr raaqflkvms dsqtlqesqn lsmflatqnk irdtvkenle kiagyeella
      241 dvvnicvhmf etkmyltpne khmlvkvmgf glflmdselc ninkldqkkk lkldridrif
      301 knlevvplfg dmqiapfnyi krskhfdask wplssssnsi spqadlmvhl pqiredhvky
      361 iselarysne vtttykecgs daenretael alrglqllsq wtsvvtelys wkllhptdhh
      421 mnkecpqeae eyeratryny tdeekfalie viamikglqv lmarmetvfi dairrniyae
      481 lqdfvqlilr eplrkaiknk kdlirsiivs vretcadwhf gveplgdpal kgkkdpdngf
      541 gikvprrnvg psstqlymvr tmlesliadk sggkrtlrkd idgqylvqid qfhktsfyws
      601 yllnfseslq dccdlsqlwy refylemtmg rkiqkcqvrh qhneecsdli tmekriqfpi
      661 emsmpwiltd hilrskepsm meyvlypldl yndsalyalt ifrkqflyde veaevnlcfd
      721 qfvyklseqi fahykqlaas illdkrfrve cvalgayllp ypranryetl lkqrhvqllg
      781 rsidlnklit qrinadmqks ldlaiskfes gditgvvele gllqvnrlth kllskwlald
      841 eydamfrean hnvlapygri tlhvfwelny dflpsycyna atnrfvkcrg lqfvqpvhrd
      901 kppqmshhyl wgskqlnlay stqygqysgf vgpqhfrtmc kllgyqgiav vmeellkivk
      961 sliqgslhqf tktlmeampk vcklprydyg spgvlgyyha qlndivqypd aktelfhnfr
     1021 efgntilfcl lmeqalsqee vcdllhaapf qnilprpyck egekpetkqk rleakyaalq
     1081 ivpnvdklgt akqamiareg dlltrerlcc glsifevvls rlhsflndpi wvgpppangv
     1141 mnvdectefh rlwsalqfvy cipvgeteft veelfgeglh wagctmivll gqqrrfeald
     1201 fcyhilrvqr vdgkdenvkg ihlkrmvdri rrfqvlnsqi favlnkylks gdsdatsveh
     1261 vrcfpppihp slahaqqqhy htpeylrein hq
//
LOCUS       XP_034196334            1292 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic FMR1-interacting protein Sra-1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196334
VERSION     XP_034196334.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340443.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1292
                     /product="cytoplasmic FMR1-interacting protein Sra-1"
                     /calculated_mol_wt=149818
     Region          386..1244
                     /region_name="FragX_IP"
                     /note="Cytoplasmic Fragile-X interacting family;
                     pfam05994"
                     /db_xref="CDD:461799"
     CDS             1..1292
                     /gene="Sra-1"
                     /coded_by="XM_034340443.2:369..4247"
                     /db_xref="GeneID:117611933"
ORIGIN      
        1 matdkvtlsd alsnvdvlde ftlpyeqpci eaqpcsivyq anfdtnfedr ngfvtgiaky
       61 ieeatvhasl nelleeglkh avmlytwrcc sraipqpksn eqpnrveiye ktvevlapev
      121 nkllnfmyfq rkaierfsge vkrlchhekr kdfvseayll tlgkfinmfa vldelknmks
      181 svkndystyr raaqflkvms dsqtlqesqn lsmflatqnk irdtvkenle kiagyeella
      241 dvvnicvhmf etkmyltpne khmlvkvmgf glflmdselc ninkldqkkk lkldridrif
      301 knlevvplfg dmqiapfnyi krskhfdask wplssssnsi spqadlmvhl pqiredhvky
      361 iselarysne vtttykecgs daenretael alrglqllsq wtsvvtelys wkllhptdhh
      421 mnkecpqeae eyeratryny tdeekfalie viamikglqv lmarmetvfi dairrniyae
      481 lqdfvqlilr eplrkaiknk kdlirsiivs vretcadwhf gveplgdpal kgkkdpdngf
      541 gikvprrnvg psstqlymvr tmlesliadk sggkrtlrkd idgqylvqid qfhktsfyws
      601 yllnfseslq dccdlsqlwy refylemtmg rkiqkcqvrh qhneecsdli tmekriqfpi
      661 emsmpwiltd hilrskepsm meyvlypldl yndsalyalt ifrkqflyde veaevnlcfd
      721 qfvyklseqi fahykqlaas illdkrfrve cvalgayllp ypranryetl lkqrhvqllg
      781 rsidlnklit qrinadmqks ldlaiskfes gditgvvele gllqvnrlth kllskwlald
      841 eydamfrean hnvlapygri tlhvfwelny dflpsycyna atnrfvkcrg lqfvqpvhrd
      901 kppqmshhyl wgskqlnlay stqygqysgf vgpqhfrtmc kllgyqgiav vmeellkivk
      961 sliqgslhqf tktlmeampk vcklprydyg spgvlgyyha qlndivqypd aktelfhnfr
     1021 efgntilfcl lmeqalsqee vcdllhaapf qnilprpyck egekpetkqk rleakyaalq
     1081 ivpnvdklgt akqamiareg dlltrerlcc glsifevvls rlhsflndpi wvgpppangv
     1141 mnvdectefh rlwsalqfvy cipvgeteft veelfgeglh wagctmivll gqqrrfeald
     1201 fcyhilrvqr vdgkdenvkg ihlkrmvdri rrfqvlnsqi favlnkylks gdsdatsveh
     1261 vrcfpppihp slahaqqqhy htpeylrein hq
//
LOCUS       XP_034196335            1032 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase TRIM33 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196335
VERSION     XP_034196335.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340444.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1032
                     /product="E3 ubiquitin-protein ligase TRIM33 isoform X1"
                     /calculated_mol_wt=116580
     Region          161..203
                     /region_name="Bbox1_TIF1"
                     /note="B-box-type 1 zinc finger found in transcription
                     intermediary factor 1 (TIF1) family; cd19805"
                     /db_xref="CDD:380863"
     Region          224..264
                     /region_name="Bbox2_TIF1_C-VI"
                     /note="B-box-type 2 zinc finger found in transcription
                     intermediary factor 1 (TIF1) family; cd19775"
                     /db_xref="CDD:380833"
     Region          270..391
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cl29238"
                     /db_xref="CDD:475168"
     Site            order(273,276..277,279..281,284,287,301..302,305,308..309,
                     312..313,316,323..324,327,330..331,334..335,338,348,351,
                     355,358..359,363..364,366..367,375,378..379,382,385..386)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467839"
     Region          <382..>705
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          828..873
                     /region_name="PHD_TIF1_like"
                     /note="PHD finger found in the transcriptional
                     intermediary factor 1 (TIF1) family and similar proteins;
                     cd15541"
                     /db_xref="CDD:277016"
     Site            order(828,833..834,839..843,850)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:277016"
     Region          892..1001
                     /region_name="Bromo_tif1_like"
                     /note="Bromodomain; tif1_like subfamily. Tif1
                     (transcription intermediary factor 1) is a member of the
                     tripartite motif (TRIM) protein family, which is
                     characterized by a particular domain architecture. It
                     functions by recruiting coactivators and/or...; cd05502"
                     /db_xref="CDD:99934"
     Site            order(921,925,928,970,974,980)
                     /site_type="other"
                     /note="putative acetyllysine binding site [chemical
                     binding]"
                     /db_xref="CDD:99934"
     CDS             1..1032
                     /gene="LOC117611934"
                     /coded_by="XM_034340444.2:132..3230"
                     /db_xref="GeneID:117611934"
ORIGIN      
        1 metrtnellt tgplatvkee pldhsgakqd mqpsqvqgvc ieeeceqrie cttdgqenet
       61 raflskcvfc gktftfgddp klleclhaac tacvnsklsd hntsvdvdvl lesnviacqi
      121 cnvttqaenl ienrflskfi eednspgtdd esketeeekk ctschdnata tswcveceef
      181 icqscvlahq rlkitkdhti kpkdevandr dnvkksskki pgclfctihs heqlslfcqt
      241 cdrltcrdcq ltehrdhkyk fiheiaaetr ssvstllkev sykrvllksa mkviedrqvl
      301 ilekkkslvq ditqmvvqlt naintrgkql vmrlnevcdq kqntlnekkv aldqlskltd
      361 hciqfvthal kkgsdmelly skksvtshlq riksrradip npeipvrihl slekvpdlik
      421 vvssigaivv dgrvypsisp lggvnapyne sqtdqkqttn lsnvhmdgss vatplpaatq
      481 psnnmqqnsy qqvplhlasq qqqqqpqsqn ymqhhplnnm pprsspqahq prvpyavnfn
      541 nrlqqplmmq qrplgschqq vtssthphqq vhidqlgqnt slrgllahnp ppfrpstnhv
      601 pyrlppyryp pnnsqrpvpp htyqppntsi smpnmrlqqc nptslstqhi nypiqqpaaa
      661 rwhipqnvnp clpcytsrhq tpsmpvptnd tykitlknqq snanqkystq tstisdnppq
      721 sqnavsvght vsssvpktps pvpagksdet eksldkfcqk slndlmltia kldsngivvi
      781 peaqrnqlds tqvdsstdeg mnpmtenssd nskntaasmt kddpnedwca vcmdggdavl
      841 ccdkcpkvfh lychipslrs fpdesetwqc mlctnvldcs ddppeerkpn tmsakelria
      901 qrivlelycq yeqslpfrev vsseivdyhr iikkpialdv irdklkskhp nhytdlrqvm
      961 adirlmfkna ftynpvesqv yqearnleef feklllkwap nyayddpfls adrdedeevf
     1021 ppnrkyrriv sd
//
LOCUS       XP_034196336             992 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase TRIM33 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196336
VERSION     XP_034196336.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340445.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..992
                     /product="E3 ubiquitin-protein ligase TRIM33 isoform X2"
                     /calculated_mol_wt=112349
     Region          161..203
                     /region_name="Bbox1_TIF1"
                     /note="B-box-type 1 zinc finger found in transcription
                     intermediary factor 1 (TIF1) family; cd19805"
                     /db_xref="CDD:380863"
     Region          224..264
                     /region_name="Bbox2_TIF1_C-VI"
                     /note="B-box-type 2 zinc finger found in transcription
                     intermediary factor 1 (TIF1) family; cd19775"
                     /db_xref="CDD:380833"
     Region          270..391
                     /region_name="CC_brat-like"
                     /note="coiled-coil (CC) domain of Drosophila brain tumor
                     (brat) and similar proteins; cl29238"
                     /db_xref="CDD:475168"
     Site            order(273,276..277,279..281,284,287,301..302,305,308..309,
                     312..313,316,323..324,327,330..331,334..335,338,348,351,
                     355,358..359,363..364,366..367,375,378..379,382,385..386)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:467839"
     Region          <426..>704
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          788..833
                     /region_name="PHD_TIF1_like"
                     /note="PHD finger found in the transcriptional
                     intermediary factor 1 (TIF1) family and similar proteins;
                     cd15541"
                     /db_xref="CDD:277016"
     Site            order(788,793..794,799..803,810)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:277016"
     Region          852..961
                     /region_name="Bromo_tif1_like"
                     /note="Bromodomain; tif1_like subfamily. Tif1
                     (transcription intermediary factor 1) is a member of the
                     tripartite motif (TRIM) protein family, which is
                     characterized by a particular domain architecture. It
                     functions by recruiting coactivators and/or...; cd05502"
                     /db_xref="CDD:99934"
     Site            order(881,885,888,930,934,940)
                     /site_type="other"
                     /note="putative acetyllysine binding site [chemical
                     binding]"
                     /db_xref="CDD:99934"
     CDS             1..992
                     /gene="LOC117611934"
                     /coded_by="XM_034340445.2:132..3110"
                     /db_xref="GeneID:117611934"
ORIGIN      
        1 metrtnellt tgplatvkee pldhsgakqd mqpsqvqgvc ieeeceqrie cttdgqenet
       61 raflskcvfc gktftfgddp klleclhaac tacvnsklsd hntsvdvdvl lesnviacqi
      121 cnvttqaenl ienrflskfi eednspgtdd esketeeekk ctschdnata tswcveceef
      181 icqscvlahq rlkitkdhti kpkdevandr dnvkksskki pgclfctihs heqlslfcqt
      241 cdrltcrdcq ltehrdhkyk fiheiaaetr ssvstllkev sykrvllksa mkviedrqvl
      301 ilekkkslvq ditqmvvqlt naintrgkql vmrlnevcdq kqntlnekkv aldqlskltd
      361 hciqfvthal kkgsdmelly skksvtshlq riksrradip npeipvrihl slekvpdlik
      421 vvssigaivv datplpaatq psnnmqqnsy qqvplhlasq qqqqqpqsqn ymqhhplnnm
      481 pprsspqahq prvpyavnfn nrlqqplmmq qrplgschqq vtssthphqq vhidqlgqnt
      541 slrgllahnp ppfrpstnhv pyrlppyryp pnnsqrpvpp htyqppntsi smpnmrlqqc
      601 nptslstqhi nypiqqpaaa rwhipqnvnp clpcytsrhq tpsmpvptnd tykitlknqq
      661 snanqkystq tstisdnppq sqnavsvght vsssvpktps pvpagksdet eksldkfcqk
      721 slndlmltia kldsngivvi peaqrnqlds tqvdsstdeg mnpmtenssd nskntaasmt
      781 kddpnedwca vcmdggdavl ccdkcpkvfh lychipslrs fpdesetwqc mlctnvldcs
      841 ddppeerkpn tmsakelria qrivlelycq yeqslpfrev vsseivdyhr iikkpialdv
      901 irdklkskhp nhytdlrqvm adirlmfkna ftynpvesqv yqearnleef feklllkwap
      961 nyayddpfls adrdedeevf ppnrkyrriv sd
//
LOCUS       XP_034196337             433 aa            linear   INV 03-SEP-2025
DEFINITION  WD repeat-containing protein 55 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034196337
VERSION     XP_034196337.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340446.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="WD repeat-containing protein 55 homolog"
                     /calculated_mol_wt=48445
     Region          111..149
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          114..392
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Site            order(124,130,136..137,149..150,168,172,178..179,192..193,
                     209,214,220..221,236,253,258,264..265,277..278,296,300,
                     306..307,339,343,349..350,362..363,381,385,391..392)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          154..190
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          198..234
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          240..277
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          284..319
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          326..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          367..391
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..433
                     /gene="LOC117611935"
                     /coded_by="XM_034340446.2:117..1418"
                     /db_xref="GeneID:117611935"
ORIGIN      
        1 mmhlykenli ddstldsdes nnsyddndsd ssmqslsedd nlsssddses enneensvsn
       61 nnqtcsytgn dmesnllned eeedevvkai irarntnsnh ppsiitedhv vdtcfhpnsd
      121 miavanivgd vhlykysnte telvstlelh lkacrdiefn esgktlfsta kdlcimltdv
      181 etekltrlye nahtepiytm tiigenmfat gdddgvvklw dlrqkentpv fslkkmedyv
      241 saivtneeak ylvcasgdgs lttfnipgkk mhvqseeyee eltclglfks etkilvstsk
      301 gkmyiynwge fglhsdefps ltkkaincmi pitdnvvitg gedgmlrats lfprrnlgvv
      361 gqhdlsietl dvnrdgtlia ssshnndikf wnvqyfetln vtepvkggkq kqlkhnlpss
      421 kidnasnffs dlq
//
LOCUS       XP_034196338             277 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase PGAM5, mitochondrial [Osmia
            lignaria lignaria].
ACCESSION   XP_034196338
VERSION     XP_034196338.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340447.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..277
                     /product="serine/threonine-protein phosphatase PGAM5,
                     mitochondrial"
                     /calculated_mol_wt=31800
     Region          <50..276
                     /region_name="HP"
                     /note="Histidine phosphatase domain found in a
                     functionally diverse set of proteins, mostly phosphatases;
                     contains a His residue which is phosphorylated during the
                     reaction; cl11399"
                     /db_xref="CDD:472174"
     Site            order(96..97,144,220..221)
                     /site_type="active"
                     /note="catalytic core [active]"
                     /db_xref="CDD:132718"
     CDS             1..277
                     /gene="LOC117611936"
                     /coded_by="XM_034340447.2:58..891"
                     /db_xref="GeneID:117611936"
ORIGIN      
        1 mpvssnfrkw itgfgaissa vffhpndndn nevpqednpw mplssswakw nhnwdrrdpk
       61 slinpkkags gidentynke lihktskaic hiilirhgqy ntkakteaeg tltdlgrqqa
      121 eitgkrlqel gfpynslihs tmiraqetae iiqknlknve vkndsllneg apiqpepmih
      181 wrpevnffrd gprieaafrk yfhradpsqe kdsytilvch anviryfvcr alqfppqswf
      241 rlslshasit witiypdgiv tlwifgdtah mkpqfis
//
LOCUS       XP_034195669            3321 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195669
VERSION     XP_034195669.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339778.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3321
                     /product="A kinase anchor protein rugose isoform X1"
                     /calculated_mol_wt=373152
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..950
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1011..1429
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2323..2488
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2520..2617
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2649..2925
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2964..3310
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3060..3096
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3102..3153
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3158..3193
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3199..3234
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3240..3275
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3321
                     /gene="rg"
                     /coded_by="XM_034339778.2:2376..12341"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlfyf sfltwqlldh
      601 vlfnpalwiy tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr
      661 aksgitpkgl dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede
      721 nlhdvlqmli slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst
      781 hkrkydvmsp hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy
      841 rlenpmilkv vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli
      901 amayihpknt eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq
      961 faqmyehyer qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv
     1021 ernykefdaa egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr
     1081 lsdkpetptt arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss
     1141 spdsmtvqev lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv
     1201 adntsdtaqa kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes
     1261 stdvvastes vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq
     1321 dnlsemkskv letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet
     1381 ceekvdveek kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn
     1441 vsdnqsdipv rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd
     1501 gvpqivsstv vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp
     1561 lrktsspqkr prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi
     1621 hqrllsdvlf sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg
     1681 llpllasats pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm
     1741 ssggilrqcl rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn
     1801 ivdnlahqls pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky
     1861 rdileppisq rppspvqtiq tngaglrpgs psadgngggg grplfpqwsh hvypqflpgs
     1921 hpnananana nanhhmnqhh hqhplpaarh snrqppsnyy lpnhhsnhyn hhpnnhnyhr
     1981 hhhyhhhnnn nhhhtlqkhi dqprnlchrn sgvglqgsgg imsqagsqdd geyeviivde
     2041 nnssiladhd vtssgppstk gghsgvprpr tiledytsse ptlgsstrte plprniqsnd
     2101 ssedtvhrsn itadpspsev ttdnenkhns seeawtdvnl nedgdtipmt nqredprnih
     2161 nmthsrgdmq dnegnvldqe mlgnaergek psaeisvvrv pdrlvmtsas prpddlpikg
     2221 lvdhlpvptp sreasltqkl emalgsvcpl lreimvdfap flsktlvgsh gqellmegkg
     2281 rgrlttfkns nsvvelvmll csqewqnslq khaglafiel inegrllsha mkdhivrvan
     2341 eaefilnrmr addvlkhadf esqcaqtlld rreeermcdh litaarrrdn viasrllekv
     2401 rnilsnkhga wgymdpmaak laeywkldaw eddarrrkrf vhnplgsshp eatlkaaleh
     2461 gapedailqa reefhahlaa srahqqqlqs adlmddsell sddrdldndl tgpvnistkg
     2521 kliapgivap giisvtstel yfevdeddpe fkkidsevlk ycdhlhgkwy fsevraifsr
     2581 ryllqnvaie iflasrtsil fafpdqatvk kvikalprvg vgikygiqqt rrasmmsprq
     2641 lmrssnmtqk wqrreisnfe ylmflntiag rtyndlnqyp vfpwvltnye tkeldlslps
     2701 nyrdlskpig alnpsrrayf eerfqswehd sippfhygth ystaafvlnw mirvepmttm
     2761 flalqggkfd hpnrlfssia lswkncqrdt sdvkelipef fflpemlvns nryrlgrqed
     2821 gssvgdvelp pwasspeefi rinrmalese fvscqlhqwi dlifgykqkg peavratnvf
     2881 yyltyegsvd ldtitdplmr eaienqirnf gqtpsqllme phpprssamh lstakfdktl
     2941 ppvlqspmmf ssipddvcmt ikfpsnspic hisantypql plpsvvtvtt gqqfavnrwn
     3001 tnyaasvqsp syadtpqaqa anqpmsmdpv lsqaanssnp alrrhlgdnf sqkltirsnc
     3061 fvttvdsrfl vacgfwdnsf rvfstetaki vqivfghygv vtclsrsecn itsdcyiasg
     3121 sadctvllwh wnartqtivg egeapaprat ltgheqpvta vvisaelglv vsgsyygpvl
     3181 vhttfgdllr sleapngfss peniamsreg vivvnyergh iaaftingkr lrheshndnl
     3241 qclllsrdge ylmtggdkri vevwrtfnla llyafpaces svrslalshd qkfllaglan
     3301 gsivifhidf nrwhhefqqr y
//
LOCUS       XP_076546829            3318 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546829
VERSION     XP_076546829.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690714.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3318
                     /product="A kinase anchor protein rugose isoform X2"
                     /calculated_mol_wt=372782
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..950
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1011..>1456
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2320..2485
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2517..2614
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2646..2922
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2961..3307
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3057..3093
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3099..3150
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3155..3190
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3196..3231
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3237..3272
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3318
                     /gene="rg"
                     /coded_by="XM_076690714.1:2376..12332"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlfyf sfltwqlldh
      601 vlfnpalwiy tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr
      661 aksgitpkgl dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede
      721 nlhdvlqmli slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst
      781 hkrkydvmsp hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy
      841 rlenpmilkv vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli
      901 amayihpknt eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq
      961 faqmyehyer qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv
     1021 ernykefdaa egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr
     1081 lsdkpetptt arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss
     1141 spdsmtvqev lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv
     1201 adntsdtaqa kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes
     1261 stdvvastes vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq
     1321 dnlsemkskv letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet
     1381 ceekvdveek kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn
     1441 vsdnqsdipv rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd
     1501 gvpqivsstv vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp
     1561 lrktsspqkr prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi
     1621 hqrllsdvlf sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg
     1681 llpllasats pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm
     1741 ssggilrqcl rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn
     1801 ivdnlahqls pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky
     1861 rdileppisq rppspvqtiq tngaglrpgs psadgngggg grplfpqwsh hvypqflpgs
     1921 hpnananana nanhhmnqhh hqhplpaarh snrqppsnyy lpnhhsnhyn hhpnnhnyhr
     1981 hhhyhhhnnn nhhhtlqkhi dqprnlchrn sgvglqgsgg imsqagsqdd geyeviivde
     2041 nnssiladhd vtssgppstk gghsgvprpr tiledytsse ptlgsstrte plprniqsnd
     2101 ssedtvhrsn itadpspsev ttdnenkhns seeawtdvnl nedgdtipmt nqredprnih
     2161 nmthsrgdmq dnegnvldqe mlgnaergek psaeisvvrv pdrlvmtsas prpddlpikg
     2221 lvdhlpvptp sreasltqkl emalgsvcpl lreimvdfap flsktlvgsh gqellmegkg
     2281 lttfknsnsv velvmllcsq ewqnslqkha glafieline grllshamkd hivrvaneae
     2341 filnrmradd vlkhadfesq caqtlldrre eermcdhlit aarrrdnvia srllekvrni
     2401 lsnkhgawgy mdpmaaklae ywkldawedd arrrkrfvhn plgsshpeat lkaalehgap
     2461 edailqaree fhahlaasra hqqqlqsadl mddsellsdd rdldndltgp vnistkgkli
     2521 apgivapgii svtstelyfe vdeddpefkk idsevlkycd hlhgkwyfse vraifsrryl
     2581 lqnvaieifl asrtsilfaf pdqatvkkvi kalprvgvgi kygiqqtrra smmsprqlmr
     2641 ssnmtqkwqr reisnfeylm flntiagrty ndlnqypvfp wvltnyetke ldlslpsnyr
     2701 dlskpigaln psrrayfeer fqswehdsip pfhygthyst aafvlnwmir vepmttmfla
     2761 lqggkfdhpn rlfssialsw kncqrdtsdv kelipefffl pemlvnsnry rlgrqedgss
     2821 vgdvelppwa sspeefirin rmalesefvs cqlhqwidli fgykqkgpea vratnvfyyl
     2881 tyegsvdldt itdplmreai enqirnfgqt psqllmephp prssamhlst akfdktlppv
     2941 lqspmmfssi pddvcmtikf psnspichis antypqlplp svvtvttgqq favnrwntny
     3001 aasvqspsya dtpqaqaanq pmsmdpvlsq aanssnpalr rhlgdnfsqk ltirsncfvt
     3061 tvdsrflvac gfwdnsfrvf stetakivqi vfghygvvtc lsrsecnits dcyiasgsad
     3121 ctvllwhwna rtqtivgege apapratltg heqpvtavvi saelglvvsg syygpvlvht
     3181 tfgdllrsle apngfsspen iamsregviv vnyerghiaa ftingkrlrh eshndnlqcl
     3241 llsrdgeylm tggdkrivev wrtfnlally afpacessvr slalshdqkf llaglangsi
     3301 vifhidfnrw hhefqqry
//
LOCUS       XP_034195673            3311 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195673
VERSION     XP_034195673.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339782.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3311
                     /product="A kinase anchor protein rugose isoform X3"
                     /calculated_mol_wt=371818
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..940
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1001..>1446
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2313..2478
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2510..2607
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2639..2915
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2954..3300
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3050..3086
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3092..3143
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3148..3183
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3189..3224
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3230..3265
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3311
                     /gene="rg"
                     /coded_by="XM_034339782.2:2375..12310"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlldh vlfnpalwiy
      601 tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr aksgitpkgl
      661 dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede nlhdvlqmli
      721 slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst hkrkydvmsp
      781 hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy rlenpmilkv
      841 vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli amayihpknt
      901 eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq faqmyehyer
      961 qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv ernykefdaa
     1021 egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr lsdkpetptt
     1081 arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss spdsmtvqev
     1141 lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv adntsdtaqa
     1201 kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes stdvvastes
     1261 vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq dnlsemkskv
     1321 letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet ceekvdveek
     1381 kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn vsdnqsdipv
     1441 rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd gvpqivsstv
     1501 vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp lrktsspqkr
     1561 prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi hqrllsdvlf
     1621 sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg llpllasats
     1681 pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm ssggilrqcl
     1741 rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn ivdnlahqls
     1801 pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky rdileppisq
     1861 rppspvqtiq tngaglrpgs psadgngggg grplfpqwsh hvypqflpgs hpnananana
     1921 nanhhmnqhh hqhplpaarh snrqppsnyy lpnhhsnhyn hhpnnhnyhr hhhyhhhnnn
     1981 nhhhtlqkhi dqprnlchrn sgvglqgsgg imsqagsqdd geyeviivde nnssiladhd
     2041 vtssgppstk gghsgvprpr tiledytsse ptlgsstrte plprniqsnd ssedtvhrsn
     2101 itadpspsev ttdnenkhns seeawtdvnl nedgdtipmt nqredprnih nmthsrgdmq
     2161 dnegnvldqe mlgnaergek psaeisvvrv pdrlvmtsas prpddlpikg lvdhlpvptp
     2221 sreasltqkl emalgsvcpl lreimvdfap flsktlvgsh gqellmegkg rgrlttfkns
     2281 nsvvelvmll csqewqnslq khaglafiel inegrllsha mkdhivrvan eaefilnrmr
     2341 addvlkhadf esqcaqtlld rreeermcdh litaarrrdn viasrllekv rnilsnkhga
     2401 wgymdpmaak laeywkldaw eddarrrkrf vhnplgsshp eatlkaaleh gapedailqa
     2461 reefhahlaa srahqqqlqs adlmddsell sddrdldndl tgpvnistkg kliapgivap
     2521 giisvtstel yfevdeddpe fkkidsevlk ycdhlhgkwy fsevraifsr ryllqnvaie
     2581 iflasrtsil fafpdqatvk kvikalprvg vgikygiqqt rrasmmsprq lmrssnmtqk
     2641 wqrreisnfe ylmflntiag rtyndlnqyp vfpwvltnye tkeldlslps nyrdlskpig
     2701 alnpsrrayf eerfqswehd sippfhygth ystaafvlnw mirvepmttm flalqggkfd
     2761 hpnrlfssia lswkncqrdt sdvkelipef fflpemlvns nryrlgrqed gssvgdvelp
     2821 pwasspeefi rinrmalese fvscqlhqwi dlifgykqkg peavratnvf yyltyegsvd
     2881 ldtitdplmr eaienqirnf gqtpsqllme phpprssamh lstakfdktl ppvlqspmmf
     2941 ssipddvcmt ikfpsnspic hisantypql plpsvvtvtt gqqfavnrwn tnyaasvqsp
     3001 syadtpqaqa anqpmsmdpv lsqaanssnp alrrhlgdnf sqkltirsnc fvttvdsrfl
     3061 vacgfwdnsf rvfstetaki vqivfghygv vtclsrsecn itsdcyiasg sadctvllwh
     3121 wnartqtivg egeapaprat ltgheqpvta vvisaelglv vsgsyygpvl vhttfgdllr
     3181 sleapngfss peniamsreg vivvnyergh iaaftingkr lrheshndnl qclllsrdge
     3241 ylmtggdkri vevwrtfnla llyafpaces svrslalshd qkfllaglan gsivifhidf
     3301 nrwhhefqqr y
//
LOCUS       XP_076546830            3307 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546830
VERSION     XP_076546830.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690715.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3307
                     /product="A kinase anchor protein rugose isoform X4"
                     /calculated_mol_wt=371625
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..950
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1011..1429
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2323..2488
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2520..2617
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2649..2925
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2950..3296
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3046..3082
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3088..3139
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3144..3179
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3185..3220
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3226..3261
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3307
                     /gene="rg"
                     /coded_by="XM_076690715.1:2375..12298"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlfyf sfltwqlldh
      601 vlfnpalwiy tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr
      661 aksgitpkgl dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede
      721 nlhdvlqmli slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst
      781 hkrkydvmsp hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy
      841 rlenpmilkv vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli
      901 amayihpknt eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq
      961 faqmyehyer qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv
     1021 ernykefdaa egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr
     1081 lsdkpetptt arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss
     1141 spdsmtvqev lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv
     1201 adntsdtaqa kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes
     1261 stdvvastes vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq
     1321 dnlsemkskv letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet
     1381 ceekvdveek kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn
     1441 vsdnqsdipv rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd
     1501 gvpqivsstv vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp
     1561 lrktsspqkr prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi
     1621 hqrllsdvlf sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg
     1681 llpllasats pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm
     1741 ssggilrqcl rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn
     1801 ivdnlahqls pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky
     1861 rdileppisq rppspvqtiq tngaglrpgs psadgngggg grplfpqwsh hvypqflpgs
     1921 hpnananana nanhhmnqhh hqhplpaarh snrqppsnyy lpnhhsnhyn hhpnnhnyhr
     1981 hhhyhhhnnn nhhhtlqkhi dqprnlchrn sgvglqgsgg imsqagsqdd geyeviivde
     2041 nnssiladhd vtssgppstk gghsgvprpr tiledytsse ptlgsstrte plprniqsnd
     2101 ssedtvhrsn itadpspsev ttdnenkhns seeawtdvnl nedgdtipmt nqredprnih
     2161 nmthsrgdmq dnegnvldqe mlgnaergek psaeisvvrv pdrlvmtsas prpddlpikg
     2221 lvdhlpvptp sreasltqkl emalgsvcpl lreimvdfap flsktlvgsh gqellmegkg
     2281 rgrlttfkns nsvvelvmll csqewqnslq khaglafiel inegrllsha mkdhivrvan
     2341 eaefilnrmr addvlkhadf esqcaqtlld rreeermcdh litaarrrdn viasrllekv
     2401 rnilsnkhga wgymdpmaak laeywkldaw eddarrrkrf vhnplgsshp eatlkaaleh
     2461 gapedailqa reefhahlaa srahqqqlqs adlmddsell sddrdldndl tgpvnistkg
     2521 kliapgivap giisvtstel yfevdeddpe fkkidsevlk ycdhlhgkwy fsevraifsr
     2581 ryllqnvaie iflasrtsil fafpdqatvk kvikalprvg vgikygiqqt rrasmmsprq
     2641 lmrssnmtqk wqrreisnfe ylmflntiag rtyndlnqyp vfpwvltnye tkeldlslps
     2701 nyrdlskpig alnpsrrayf eerfqswehd sippfhygth ystaafvlnw mirvepmttm
     2761 flalqggkfd hpnrlfssia lswkncqrdt sdvkelipef fflpemlvns nryrlgrqed
     2821 gssvgdvelp pwasspeefi rinrmalese fvscqlhqwi dlifgykqkg peavratnvf
     2881 yyltyegsvd ldtitdplmr eaienqirnf gqtpsqllme phpprssamh lspmmfssip
     2941 ddvcmtikfp snspichisa ntypqlplps vvtvttgqqf avnrwntnya asvqspsyad
     3001 tpqaqaanqp msmdpvlsqa anssnpalrr hlgdnfsqkl tirsncfvtt vdsrflvacg
     3061 fwdnsfrvfs tetakivqiv fghygvvtcl srsecnitsd cyiasgsadc tvllwhwnar
     3121 tqtivgegea papratltgh eqpvtavvis aelglvvsgs yygpvlvhtt fgdllrslea
     3181 pngfsspeni amsregvivv nyerghiaaf tingkrlrhe shndnlqcll lsrdgeylmt
     3241 ggdkrivevw rtfnlallya fpacessvrs lalshdqkfl laglangsiv ifhidfnrwh
     3301 hefqqry
//
LOCUS       XP_076546831            3306 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546831
VERSION     XP_076546831.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690716.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3306
                     /product="A kinase anchor protein rugose isoform X5"
                     /calculated_mol_wt=371691
     Region          210..358
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          439..935
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <996..>1441
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2308..2473
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2505..2602
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2634..2910
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2949..3295
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3045..3081
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3087..3138
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3143..3178
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3184..3219
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3225..3260
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3306
                     /gene="rg"
                     /coded_by="XM_076690716.1:212..10132"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 madmksppfs dikrpeevva mamndglkfa vliglievgq vsnrevvntv lhllvggefd
       61 melnfviqda qnirhmlell dhcpanlqae iwsvfiailr ksvrnlqact dvsliehvlh
      121 rlsraetvva dllidmlgvl asysitvkel kllfgamkav kgkwprhsak llnvlrqmpq
      181 rngpdvffsf pgrkgsaivl pplakwphen gftfttwfrl dpinsvnier ekpylycfkt
      241 skgvgysahf vgnclvltsm kvkgkgfqhc vkyefqprkw ymiavvyiyn rwtkseikcl
      301 vngqlasste mawfvstndp fdkcyigatp evdeervfcg qmsaiylfse altthqicam
      361 hrlgpgyksq frfdnecyln lpdnhkrvsd epelasmvdq smqtvlydgk lsnaivfmyn
      421 pvatdsqlcl qsapkgnvsy fvhtphalml qdvkaviths ihstlnsigg iqvlfplfsq
      481 ldmpydciap ndvkrdptlc skllgficdl vessqtvqqh mvqnrgflvi symlqrasrd
      541 hlttevlasf leltkhlvtc lsansdlllk qlfyfsfltw qlldhvlfnp alwiytpapv
      601 qtrlysylat eflsdtqiys nvrrvstvlq tvhtlkyyyw vanpraksgi tpkgldgprp
      661 qqkdiltirs yillflkqli migngvkdde lqsilnyltt ihedenlhdv lqmlislmse
      721 hpssmvpafd akqgvrtifk llaaesqlir lqalkllgff lsrsthkrky dvmsphnlyt
      781 llaerlllne etlslptynv lyeimtehis qqilyvrhpe peshyrlenp milkvvatli
      841 rqskqteqll evkklflsdm tllcnnnren rrtvlqmsvw qewliamayi hpknteeqki
      901 sdmvyslfrm llhhaikhey ggwrvwvdtl aivhskvsye efklqfaqmy ehyerqrsdn
      961 itdpelrqqr pistisgwdq qhsgnngynk pspwgnqqnh dvqhvernyk efdaaegndr
     1021 leescscdln sidntesdgs paivksnset ldtpqsseai sldsrlsdkp etpttarevh
     1081 tdtptileeg nseaasvptt qetseaisie sssdfysevh kidssspdsm tvqevlkkde
     1141 epseqkeedt vsesasakqt eenvqaeqed avpeekstpk enanvadnts dtaqakedte
     1201 asstetkdee tskekseaad dvplnddvps ntevdsekaa atdesstdvv astesvdsen
     1261 ipsaspgetp epliikdiek lqlendreii dsdtseaylt ptenqdnlse mkskvletek
     1321 iednmvedae dtksttvkde nkendneetv knpncstsvi entetceekv dveekkvesv
     1381 vasselssek cavdsvtede savindndek viekhsreps tistnvsdnq sdipvrtete
     1441 vevtdsvcsn sdvqssvdnv tqvpnpkqqi ptistvcdan tnmpdgvpqi vsstvvtrdn
     1501 sllndltpdh vdthrrsslp avptetaned nvnsnnnepp slpvplrkts spqkrprsas
     1561 tstqvdpnhf etkrskqgnp strpmfspgp trppfripef kwsyihqrll sdvlfsletd
     1621 iqvwrshstk svldfvnsse naifvvntvh lisqladnli iacggllpll asatspnsel
     1681 dvieptqgmp ievavsflqr lvnmadvlif asslnfgele aeknmssggi lrqclrlvct
     1741 cavrncleck ergrpysmlg rqigtsnksq hiqslirgaq tspknivdnl ahqlspvkdp
     1801 ekllqdmdvn rlraviyrdv eetkqaqfls laivyfisvl mvskyrdile ppisqrppsp
     1861 vqtiqtngag lrpgspsadg ngggggrplf pqwshhvypq flpgshpnan ananananhh
     1921 mnqhhhqhpl paarhsnrqp psnyylpnhh snhynhhpnn hnyhrhhhyh hhnnnnhhht
     1981 lqkhidqprn lchrnsgvgl qgsggimsqa gsqddgeyev iivdennssi ladhdvtssg
     2041 ppstkgghsg vprprtiled ytsseptlgs strteplprn iqsndssedt vhrsnitadp
     2101 spsevttdne nkhnsseeaw tdvnlnedgd tipmtnqred prnihnmths rgdmqdnegn
     2161 vldqemlgna ergekpsaei svvrvpdrlv mtsasprpdd lpikglvdhl pvptpsreas
     2221 ltqklemalg svcpllreim vdfapflskt lvgshgqell megkgrgrlt tfknsnsvve
     2281 lvmllcsqew qnslqkhagl afielinegr llshamkdhi vrvaneaefi lnrmraddvl
     2341 khadfesqca qtlldrreee rmcdhlitaa rrrdnviasr llekvrnils nkhgawgymd
     2401 pmaaklaeyw kldaweddar rrkrfvhnpl gsshpeatlk aalehgaped ailqareefh
     2461 ahlaasrahq qqlqsadlmd dsellsddrd ldndltgpvn istkgkliap givapgiisv
     2521 tstelyfevd eddpefkkid sevlkycdhl hgkwyfsevr aifsrryllq nvaieiflas
     2581 rtsilfafpd qatvkkvika lprvgvgiky giqqtrrasm msprqlmrss nmtqkwqrre
     2641 isnfeylmfl ntiagrtynd lnqypvfpwv ltnyetkeld lslpsnyrdl skpigalnps
     2701 rrayfeerfq swehdsippf hygthystaa fvlnwmirve pmttmflalq ggkfdhpnrl
     2761 fssialswkn cqrdtsdvke lipeffflpe mlvnsnryrl grqedgssvg dvelppwass
     2821 peefirinrm alesefvscq lhqwidlifg ykqkgpeavr atnvfyylty egsvdldtit
     2881 dplmreaien qirnfgqtps qllmephppr ssamhlstak fdktlppvlq spmmfssipd
     2941 dvcmtikfps nspichisan typqlplpsv vtvttgqqfa vnrwntnyaa svqspsyadt
     3001 pqaqaanqpm smdpvlsqaa nssnpalrrh lgdnfsqklt irsncfvttv dsrflvacgf
     3061 wdnsfrvfst etakivqivf ghygvvtcls rsecnitsdc yiasgsadct vllwhwnart
     3121 qtivgegeap apratltghe qpvtavvisa elglvvsgsy ygpvlvhttf gdllrsleap
     3181 ngfsspenia msregvivvn yerghiaaft ingkrlrhes hndnlqclll srdgeylmtg
     3241 gdkrivevwr tfnlallyaf pacessvrsl alshdqkfll aglangsivi fhidfnrwhh
     3301 efqqry
//
LOCUS       XP_076546832            3305 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546832
VERSION     XP_076546832.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690717.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3305
                     /product="A kinase anchor protein rugose isoform X6"
                     /calculated_mol_wt=371620
     Region          210..358
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          439..935
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <996..>1441
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2307..2472
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2504..2601
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2633..2909
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2948..3294
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3044..3080
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3086..3137
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3142..3177
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3183..3218
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3224..3259
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3305
                     /gene="rg"
                     /coded_by="XM_076690717.1:212..10129"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 madmksppfs dikrpeevva mamndglkfa vliglievgq vsnrevvntv lhllvggefd
       61 melnfviqda qnirhmlell dhcpanlqae iwsvfiailr ksvrnlqact dvsliehvlh
      121 rlsraetvva dllidmlgvl asysitvkel kllfgamkav kgkwprhsak llnvlrqmpq
      181 rngpdvffsf pgrkgsaivl pplakwphen gftfttwfrl dpinsvnier ekpylycfkt
      241 skgvgysahf vgnclvltsm kvkgkgfqhc vkyefqprkw ymiavvyiyn rwtkseikcl
      301 vngqlasste mawfvstndp fdkcyigatp evdeervfcg qmsaiylfse altthqicam
      361 hrlgpgyksq frfdnecyln lpdnhkrvsd epelasmvdq smqtvlydgk lsnaivfmyn
      421 pvatdsqlcl qsapkgnvsy fvhtphalml qdvkaviths ihstlnsigg iqvlfplfsq
      481 ldmpydciap ndvkrdptlc skllgficdl vessqtvqqh mvqnrgflvi symlqrasrd
      541 hlttevlasf leltkhlvtc lsansdlllk qlfyfsfltw qlldhvlfnp alwiytpapv
      601 qtrlysylat eflsdtqiys nvrrvstvlq tvhtlkyyyw vanpraksgi tpkgldgprp
      661 qqkdiltirs yillflkqli migngvkdde lqsilnyltt ihedenlhdv lqmlislmse
      721 hpssmvpafd akqgvrtifk llaaesqlir lqalkllgff lsrsthkrky dvmsphnlyt
      781 llaerlllne etlslptynv lyeimtehis qqilyvrhpe peshyrlenp milkvvatli
      841 rqskqteqll evkklflsdm tllcnnnren rrtvlqmsvw qewliamayi hpknteeqki
      901 sdmvyslfrm llhhaikhey ggwrvwvdtl aivhskvsye efklqfaqmy ehyerqrsdn
      961 itdpelrqqr pistisgwdq qhsgnngynk pspwgnqqnh dvqhvernyk efdaaegndr
     1021 leescscdln sidntesdgs paivksnset ldtpqsseai sldsrlsdkp etpttarevh
     1081 tdtptileeg nseaasvptt qetseaisie sssdfysevh kidssspdsm tvqevlkkde
     1141 epseqkeedt vsesasakqt eenvqaeqed avpeekstpk enanvadnts dtaqakedte
     1201 asstetkdee tskekseaad dvplnddvps ntevdsekaa atdesstdvv astesvdsen
     1261 ipsaspgetp epliikdiek lqlendreii dsdtseaylt ptenqdnlse mkskvletek
     1321 iednmvedae dtksttvkde nkendneetv knpncstsvi entetceekv dveekkvesv
     1381 vasselssek cavdsvtede savindndek viekhsreps tistnvsdnq sdipvrtete
     1441 vevtdsvcsn sdvqssvdnv tqvpnpkqqi ptistvcdan tnmpdgvpqi vsstvvtrdn
     1501 sllndltpdh vdthrrsslp avptetaned nvnsnnnepp slpvplrkts spqkrprsas
     1561 tstqvdpnhf etkrskqgnp strpmfspgp trppfripef kwsyihqrll sdvlfsletd
     1621 iqvwrshstk svldfvnsse naifvvntvh lisqladnli iacggllpll asatspnsel
     1681 dvieptqgmp ievavsflqr lvnmadvlif asslnfgele aeknmssggi lrqclrlvct
     1741 cavrncleck ergrpysmlg rqigtsnksq hiqslirgaq tspknivdnl ahqlspvkdp
     1801 ekllqdmdvn rlraviyrdv eetkqaqfls laivyfisvl mvskyrdile ppisqrppsp
     1861 vqtiqtngag lrpgspsdgn gggggrplfp qwshhvypqf lpgshpnana nanananhhm
     1921 nqhhhqhplp aarhsnrqpp snyylpnhhs nhynhhpnnh nyhrhhhyhh hnnnnhhhtl
     1981 qkhidqprnl chrnsgvglq gsggimsqag sqddgeyevi ivdennssil adhdvtssgp
     2041 pstkgghsgv prprtiledy tsseptlgss trteplprni qsndssedtv hrsnitadps
     2101 psevttdnen khnsseeawt dvnlnedgdt ipmtnqredp rnihnmthsr gdmqdnegnv
     2161 ldqemlgnae rgekpsaeis vvrvpdrlvm tsasprpddl pikglvdhlp vptpsreasl
     2221 tqklemalgs vcpllreimv dfapflsktl vgshgqellm egkgrgrltt fknsnsvvel
     2281 vmllcsqewq nslqkhagla fielinegrl lshamkdhiv rvaneaefil nrmraddvlk
     2341 hadfesqcaq tlldrreeer mcdhlitaar rrdnviasrl lekvrnilsn khgawgymdp
     2401 maaklaeywk ldaweddarr rkrfvhnplg sshpeatlka alehgapeda ilqareefha
     2461 hlaasrahqq qlqsadlmdd sellsddrdl dndltgpvni stkgkliapg ivapgiisvt
     2521 stelyfevde ddpefkkids evlkycdhlh gkwyfsevra ifsrryllqn vaieiflasr
     2581 tsilfafpdq atvkkvikal prvgvgikyg iqqtrrasmm sprqlmrssn mtqkwqrrei
     2641 snfeylmfln tiagrtyndl nqypvfpwvl tnyetkeldl slpsnyrdls kpigalnpsr
     2701 rayfeerfqs wehdsippfh ygthystaaf vlnwmirvep mttmflalqg gkfdhpnrlf
     2761 ssialswknc qrdtsdvkel ipeffflpem lvnsnryrlg rqedgssvgd velppwassp
     2821 eefirinrma lesefvscql hqwidlifgy kqkgpeavra tnvfyyltye gsvdldtitd
     2881 plmreaienq irnfgqtpsq llmephpprs samhlstakf dktlppvlqs pmmfssipdd
     2941 vcmtikfpsn spichisant ypqlplpsvv tvttgqqfav nrwntnyaas vqspsyadtp
     3001 qaqaanqpms mdpvlsqaan ssnpalrrhl gdnfsqklti rsncfvttvd srflvacgfw
     3061 dnsfrvfste takivqivfg hygvvtclsr secnitsdcy iasgsadctv llwhwnartq
     3121 tivgegeapa pratltgheq pvtavvisae lglvvsgsyy gpvlvhttfg dllrsleapn
     3181 gfsspeniam sregvivvny erghiaafti ngkrlrhesh ndnlqcllls rdgeylmtgg
     3241 dkrivevwrt fnlallyafp acessvrsla lshdqkflla glangsivif hidfnrwhhe
     3301 fqqry
//
LOCUS       XP_076546833            3304 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546833
VERSION     XP_076546833.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690718.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3304
                     /product="A kinase anchor protein rugose isoform X7"
                     /calculated_mol_wt=371256
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..950
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1011..1429
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2320..2485
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2517..2614
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2646..2922
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2947..3293
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3043..3079
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3085..3136
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3141..3176
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3182..3217
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3223..3258
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3304
                     /gene="rg"
                     /coded_by="XM_076690718.1:2374..12288"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlfyf sfltwqlldh
      601 vlfnpalwiy tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr
      661 aksgitpkgl dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede
      721 nlhdvlqmli slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst
      781 hkrkydvmsp hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy
      841 rlenpmilkv vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli
      901 amayihpknt eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq
      961 faqmyehyer qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv
     1021 ernykefdaa egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr
     1081 lsdkpetptt arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss
     1141 spdsmtvqev lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv
     1201 adntsdtaqa kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes
     1261 stdvvastes vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq
     1321 dnlsemkskv letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet
     1381 ceekvdveek kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn
     1441 vsdnqsdipv rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd
     1501 gvpqivsstv vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp
     1561 lrktsspqkr prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi
     1621 hqrllsdvlf sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg
     1681 llpllasats pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm
     1741 ssggilrqcl rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn
     1801 ivdnlahqls pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky
     1861 rdileppisq rppspvqtiq tngaglrpgs psadgngggg grplfpqwsh hvypqflpgs
     1921 hpnananana nanhhmnqhh hqhplpaarh snrqppsnyy lpnhhsnhyn hhpnnhnyhr
     1981 hhhyhhhnnn nhhhtlqkhi dqprnlchrn sgvglqgsgg imsqagsqdd geyeviivde
     2041 nnssiladhd vtssgppstk gghsgvprpr tiledytsse ptlgsstrte plprniqsnd
     2101 ssedtvhrsn itadpspsev ttdnenkhns seeawtdvnl nedgdtipmt nqredprnih
     2161 nmthsrgdmq dnegnvldqe mlgnaergek psaeisvvrv pdrlvmtsas prpddlpikg
     2221 lvdhlpvptp sreasltqkl emalgsvcpl lreimvdfap flsktlvgsh gqellmegkg
     2281 lttfknsnsv velvmllcsq ewqnslqkha glafieline grllshamkd hivrvaneae
     2341 filnrmradd vlkhadfesq caqtlldrre eermcdhlit aarrrdnvia srllekvrni
     2401 lsnkhgawgy mdpmaaklae ywkldawedd arrrkrfvhn plgsshpeat lkaalehgap
     2461 edailqaree fhahlaasra hqqqlqsadl mddsellsdd rdldndltgp vnistkgkli
     2521 apgivapgii svtstelyfe vdeddpefkk idsevlkycd hlhgkwyfse vraifsrryl
     2581 lqnvaieifl asrtsilfaf pdqatvkkvi kalprvgvgi kygiqqtrra smmsprqlmr
     2641 ssnmtqkwqr reisnfeylm flntiagrty ndlnqypvfp wvltnyetke ldlslpsnyr
     2701 dlskpigaln psrrayfeer fqswehdsip pfhygthyst aafvlnwmir vepmttmfla
     2761 lqggkfdhpn rlfssialsw kncqrdtsdv kelipefffl pemlvnsnry rlgrqedgss
     2821 vgdvelppwa sspeefirin rmalesefvs cqlhqwidli fgykqkgpea vratnvfyyl
     2881 tyegsvdldt itdplmreai enqirnfgqt psqllmephp prssamhlsp mmfssipddv
     2941 cmtikfpsns pichisanty pqlplpsvvt vttgqqfavn rwntnyaasv qspsyadtpq
     3001 aqaanqpmsm dpvlsqaans snpalrrhlg dnfsqkltir sncfvttvds rflvacgfwd
     3061 nsfrvfstet akivqivfgh ygvvtclsrs ecnitsdcyi asgsadctvl lwhwnartqt
     3121 ivgegeapap ratltgheqp vtavvisael glvvsgsyyg pvlvhttfgd llrsleapng
     3181 fsspeniams regvivvnye rghiaaftin gkrlrheshn dnlqclllsr dgeylmtggd
     3241 krivevwrtf nlallyafpa cessvrslal shdqkfllag langsivifh idfnrwhhef
     3301 qqry
//
LOCUS       XP_034195675            3304 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195675
VERSION     XP_034195675.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339784.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3304
                     /product="A kinase anchor protein rugose isoform X8"
                     /calculated_mol_wt=371303
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          437..933
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <994..>1439
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2306..2471
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2503..2600
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2632..2908
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2947..3293
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3043..3079
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3085..3136
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3141..3176
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3182..3217
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3223..3258
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3304
                     /gene="rg"
                     /coded_by="XM_034339784.2:2376..12290"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvlydgkls naivfmynpv
      421 atdsqlclqs apkgnvsyfv htphalmlqd vkavithsih stlnsiggiq vlfplfsqld
      481 mpydciapnd vkrdptlcsk llgficdlve ssqtvqqhmv qnrgflvisy mlqrasrdhl
      541 ttevlasfle ltkhlvtcls ansdlllkql fyfsfltwql ldhvlfnpal wiytpapvqt
      601 rlysylatef lsdtqiysnv rrvstvlqtv htlkyyywva npraksgitp kgldgprpqq
      661 kdiltirsyi llflkqlimi gngvkddelq silnylttih edenlhdvlq mlislmsehp
      721 ssmvpafdak qgvrtifkll aaesqlirlq alkllgffls rsthkrkydv msphnlytll
      781 aerlllneet lslptynvly eimtehisqq ilyvrhpepe shyrlenpmi lkvvatlirq
      841 skqteqllev kklflsdmtl lcnnnrenrr tvlqmsvwqe wliamayihp knteeqkisd
      901 mvyslfrmll hhaikheygg wrvwvdtlai vhskvsyeef klqfaqmyeh yerqrsdnit
      961 dpelrqqrpi stisgwdqqh sgnngynkps pwgnqqnhdv qhvernykef daaegndrle
     1021 escscdlnsi dntesdgspa ivksnsetld tpqsseaisl dsrlsdkpet pttarevhtd
     1081 tptileegns eaasvpttqe tseaisiess sdfysevhki dssspdsmtv qevlkkdeep
     1141 seqkeedtvs esasakqtee nvqaeqedav peekstpken anvadntsdt aqakedteas
     1201 stetkdeets kekseaaddv plnddvpsnt evdsekaaat desstdvvas tesvdsenip
     1261 saspgetpep liikdieklq lendreiids dtseayltpt enqdnlsemk skvletekie
     1321 dnmvedaedt ksttvkdenk endneetvkn pncstsvien tetceekvdv eekkvesvva
     1381 sselssekca vdsvtedesa vindndekvi ekhsrepsti stnvsdnqsd ipvrteteve
     1441 vtdsvcsnsd vqssvdnvtq vpnpkqqipt istvcdantn mpdgvpqivs stvvtrdnsl
     1501 lndltpdhvd thrrsslpav ptetanednv nsnnneppsl pvplrktssp qkrprsasts
     1561 tqvdpnhfet krskqgnpst rpmfspgptr ppfripefkw syihqrllsd vlfsletdiq
     1621 vwrshstksv ldfvnssena ifvvntvhli sqladnliia cggllpllas atspnseldv
     1681 ieptqgmpie vavsflqrlv nmadvlifas slnfgeleae knmssggilr qclrlvctca
     1741 vrnclecker grpysmlgrq igtsnksqhi qslirgaqts pknivdnlah qlspvkdpek
     1801 llqdmdvnrl raviyrdvee tkqaqflsla ivyfisvlmv skyrdilepp isqrppspvq
     1861 tiqtngaglr pgspsadgng ggggrplfpq wshhvypqfl pgshpnanan anananhhmn
     1921 qhhhqhplpa arhsnrqpps nyylpnhhsn hynhhpnnhn yhrhhhyhhh nnnnhhhtlq
     1981 khidqprnlc hrnsgvglqg sggimsqags qddgeyevii vdennssila dhdvtssgpp
     2041 stkgghsgvp rprtiledyt sseptlgsst rteplprniq sndssedtvh rsnitadpsp
     2101 sevttdnenk hnsseeawtd vnlnedgdti pmtnqredpr nihnmthsrg dmqdnegnvl
     2161 dqemlgnaer gekpsaeisv vrvpdrlvmt sasprpddlp ikglvdhlpv ptpsreaslt
     2221 qklemalgsv cpllreimvd fapflsktlv gshgqellme gkgrgrlttf knsnsvvelv
     2281 mllcsqewqn slqkhaglaf ielinegrll shamkdhivr vaneaefiln rmraddvlkh
     2341 adfesqcaqt lldrreeerm cdhlitaarr rdnviasrll ekvrnilsnk hgawgymdpm
     2401 aaklaeywkl daweddarrr krfvhnplgs shpeatlkaa lehgapedai lqareefhah
     2461 laasrahqqq lqsadlmdds ellsddrdld ndltgpvnis tkgkliapgi vapgiisvts
     2521 telyfevded dpefkkidse vlkycdhlhg kwyfsevrai fsrryllqnv aieiflasrt
     2581 silfafpdqa tvkkvikalp rvgvgikygi qqtrrasmms prqlmrssnm tqkwqrreis
     2641 nfeylmflnt iagrtyndln qypvfpwvlt nyetkeldls lpsnyrdlsk pigalnpsrr
     2701 ayfeerfqsw ehdsippfhy gthystaafv lnwmirvepm ttmflalqgg kfdhpnrlfs
     2761 sialswkncq rdtsdvkeli peffflpeml vnsnryrlgr qedgssvgdv elppwasspe
     2821 efirinrmal esefvscqlh qwidlifgyk qkgpeavrat nvfyyltyeg svdldtitdp
     2881 lmreaienqi rnfgqtpsql lmephpprss amhlstakfd ktlppvlqsp mmfssipddv
     2941 cmtikfpsns pichisanty pqlplpsvvt vttgqqfavn rwntnyaasv qspsyadtpq
     3001 aqaanqpmsm dpvlsqaans snpalrrhlg dnfsqkltir sncfvttvds rflvacgfwd
     3061 nsfrvfstet akivqivfgh ygvvtclsrs ecnitsdcyi asgsadctvl lwhwnartqt
     3121 ivgegeapap ratltgheqp vtavvisael glvvsgsyyg pvlvhttfgd llrsleapng
     3181 fsspeniams regvivvnye rghiaaftin gkrlrheshn dnlqclllsr dgeylmtggd
     3241 krivevwrtf nlallyafpa cessvrslal shdqkfllag langsivifh idfnrwhhef
     3301 qqry
//
LOCUS       XP_034195677            3294 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195677
VERSION     XP_034195677.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339786.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3294
                     /product="A kinase anchor protein rugose isoform X9"
                     /calculated_mol_wt=369969
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          437..923
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <984..1402
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2296..2461
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2493..2590
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2622..2898
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2937..3283
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3033..3069
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3075..3126
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3131..3166
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3172..3207
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3213..3248
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3294
                     /gene="rg"
                     /coded_by="XM_034339786.2:2376..12260"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvlydgkls naivfmynpv
      421 atdsqlclqs apkgnvsyfv htphalmlqd vkavithsih stlnsiggiq vlfplfsqld
      481 mpydciapnd vkrdptlcsk llgficdlve ssqtvqqhmv qnrgflvisy mlqrasrdhl
      541 ttevlasfle ltkhlvtcls ansdlllkql ldhvlfnpal wiytpapvqt rlysylatef
      601 lsdtqiysnv rrvstvlqtv htlkyyywva npraksgitp kgldgprpqq kdiltirsyi
      661 llflkqlimi gngvkddelq silnylttih edenlhdvlq mlislmsehp ssmvpafdak
      721 qgvrtifkll aaesqlirlq alkllgffls rsthkrkydv msphnlytll aerlllneet
      781 lslptynvly eimtehisqq ilyvrhpepe shyrlenpmi lkvvatlirq skqteqllev
      841 kklflsdmtl lcnnnrenrr tvlqmsvwqe wliamayihp knteeqkisd mvyslfrmll
      901 hhaikheygg wrvwvdtlai vhskvsyeef klqfaqmyeh yerqrsdnit dpelrqqrpi
      961 stisgwdqqh sgnngynkps pwgnqqnhdv qhvernykef daaegndrle escscdlnsi
     1021 dntesdgspa ivksnsetld tpqsseaisl dsrlsdkpet pttarevhtd tptileegns
     1081 eaasvpttqe tseaisiess sdfysevhki dssspdsmtv qevlkkdeep seqkeedtvs
     1141 esasakqtee nvqaeqedav peekstpken anvadntsdt aqakedteas stetkdeets
     1201 kekseaaddv plnddvpsnt evdsekaaat desstdvvas tesvdsenip saspgetpep
     1261 liikdieklq lendreiids dtseayltpt enqdnlsemk skvletekie dnmvedaedt
     1321 ksttvkdenk endneetvkn pncstsvien tetceekvdv eekkvesvva sselssekca
     1381 vdsvtedesa vindndekvi ekhsrepsti stnvsdnqsd ipvrteteve vtdsvcsnsd
     1441 vqssvdnvtq vpnpkqqipt istvcdantn mpdgvpqivs stvvtrdnsl lndltpdhvd
     1501 thrrsslpav ptetanednv nsnnneppsl pvplrktssp qkrprsasts tqvdpnhfet
     1561 krskqgnpst rpmfspgptr ppfripefkw syihqrllsd vlfsletdiq vwrshstksv
     1621 ldfvnssena ifvvntvhli sqladnliia cggllpllas atspnseldv ieptqgmpie
     1681 vavsflqrlv nmadvlifas slnfgeleae knmssggilr qclrlvctca vrnclecker
     1741 grpysmlgrq igtsnksqhi qslirgaqts pknivdnlah qlspvkdpek llqdmdvnrl
     1801 raviyrdvee tkqaqflsla ivyfisvlmv skyrdilepp isqrppspvq tiqtngaglr
     1861 pgspsadgng ggggrplfpq wshhvypqfl pgshpnanan anananhhmn qhhhqhplpa
     1921 arhsnrqpps nyylpnhhsn hynhhpnnhn yhrhhhyhhh nnnnhhhtlq khidqprnlc
     1981 hrnsgvglqg sggimsqags qddgeyevii vdennssila dhdvtssgpp stkgghsgvp
     2041 rprtiledyt sseptlgsst rteplprniq sndssedtvh rsnitadpsp sevttdnenk
     2101 hnsseeawtd vnlnedgdti pmtnqredpr nihnmthsrg dmqdnegnvl dqemlgnaer
     2161 gekpsaeisv vrvpdrlvmt sasprpddlp ikglvdhlpv ptpsreaslt qklemalgsv
     2221 cpllreimvd fapflsktlv gshgqellme gkgrgrlttf knsnsvvelv mllcsqewqn
     2281 slqkhaglaf ielinegrll shamkdhivr vaneaefiln rmraddvlkh adfesqcaqt
     2341 lldrreeerm cdhlitaarr rdnviasrll ekvrnilsnk hgawgymdpm aaklaeywkl
     2401 daweddarrr krfvhnplgs shpeatlkaa lehgapedai lqareefhah laasrahqqq
     2461 lqsadlmdds ellsddrdld ndltgpvnis tkgkliapgi vapgiisvts telyfevded
     2521 dpefkkidse vlkycdhlhg kwyfsevrai fsrryllqnv aieiflasrt silfafpdqa
     2581 tvkkvikalp rvgvgikygi qqtrrasmms prqlmrssnm tqkwqrreis nfeylmflnt
     2641 iagrtyndln qypvfpwvlt nyetkeldls lpsnyrdlsk pigalnpsrr ayfeerfqsw
     2701 ehdsippfhy gthystaafv lnwmirvepm ttmflalqgg kfdhpnrlfs sialswkncq
     2761 rdtsdvkeli peffflpeml vnsnryrlgr qedgssvgdv elppwasspe efirinrmal
     2821 esefvscqlh qwidlifgyk qkgpeavrat nvfyyltyeg svdldtitdp lmreaienqi
     2881 rnfgqtpsql lmephpprss amhlstakfd ktlppvlqsp mmfssipddv cmtikfpsns
     2941 pichisanty pqlplpsvvt vttgqqfavn rwntnyaasv qspsyadtpq aqaanqpmsm
     3001 dpvlsqaans snpalrrhlg dnfsqkltir sncfvttvds rflvacgfwd nsfrvfstet
     3061 akivqivfgh ygvvtclsrs ecnitsdcyi asgsadctvl lwhwnartqt ivgegeapap
     3121 ratltgheqp vtavvisael glvvsgsyyg pvlvhttfgd llrsleapng fsspeniams
     3181 regvivvnye rghiaaftin gkrlrheshn dnlqclllsr dgeylmtggd krivevwrtf
     3241 nlallyafpa cessvrslal shdqkfllag langsivifh idfnrwhhef qqry
//
LOCUS       XP_076546834            3289 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546834
VERSION     XP_076546834.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690719.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3289
                     /product="A kinase anchor protein rugose isoform X10"
                     /calculated_mol_wt=369842
     Region          210..358
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          422..918
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <979..>1424
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2291..2456
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2488..2585
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2617..2893
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2932..3278
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3028..3064
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3070..3121
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3126..3161
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3167..3202
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3208..3243
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3289
                     /gene="rg"
                     /coded_by="XM_076690719.1:212..10081"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 madmksppfs dikrpeevva mamndglkfa vliglievgq vsnrevvntv lhllvggefd
       61 melnfviqda qnirhmlell dhcpanlqae iwsvfiailr ksvrnlqact dvsliehvlh
      121 rlsraetvva dllidmlgvl asysitvkel kllfgamkav kgkwprhsak llnvlrqmpq
      181 rngpdvffsf pgrkgsaivl pplakwphen gftfttwfrl dpinsvnier ekpylycfkt
      241 skgvgysahf vgnclvltsm kvkgkgfqhc vkyefqprkw ymiavvyiyn rwtkseikcl
      301 vngqlasste mawfvstndp fdkcyigatp evdeervfcg qmsaiylfse altthqicam
      361 hrlgpgyksq frfdnecyln lpdnhkrvly dgklsnaivf mynpvatdsq lclqsapkgn
      421 vsyfvhtpha lmlqdvkavi thsihstlns iggiqvlfpl fsqldmpydc iapndvkrdp
      481 tlcskllgfi cdlvessqtv qqhmvqnrgf lvisymlqra srdhlttevl asfleltkhl
      541 vtclsansdl llkqlfyfsf ltwqlldhvl fnpalwiytp apvqtrlysy lateflsdtq
      601 iysnvrrvst vlqtvhtlky yywvanprak sgitpkgldg prpqqkdilt irsyillflk
      661 qlimigngvk ddelqsilny lttihedenl hdvlqmlisl msehpssmvp afdakqgvrt
      721 ifkllaaesq lirlqalkll gfflsrsthk rkydvmsphn lytllaerll lneetlslpt
      781 ynvlyeimte hisqqilyvr hpepeshyrl enpmilkvva tlirqskqte qllevkklfl
      841 sdmtllcnnn renrrtvlqm svwqewliam ayihpkntee qkisdmvysl frmllhhaik
      901 heyggwrvwv dtlaivhskv syeefklqfa qmyehyerqr sdnitdpelr qqrpistisg
      961 wdqqhsgnng ynkpspwgnq qnhdvqhver nykefdaaeg ndrleescsc dlnsidntes
     1021 dgspaivksn setldtpqss eaisldsrls dkpetpttar evhtdtptil eegnseaasv
     1081 pttqetseai siesssdfys evhkidsssp dsmtvqevlk kdeepseqke edtvsesasa
     1141 kqteenvqae qedavpeeks tpkenanvad ntsdtaqake dteasstetk deetskekse
     1201 aaddvplndd vpsntevdse kaaatdesst dvvastesvd senipsaspg etpepliikd
     1261 ieklqlendr eiidsdtsea yltptenqdn lsemkskvle tekiednmve daedtksttv
     1321 kdenkendne etvknpncst svientetce ekvdveekkv esvvassels sekcavdsvt
     1381 edesavindn dekviekhsr epstistnvs dnqsdipvrt etevevtdsv csnsdvqssv
     1441 dnvtqvpnpk qqiptistvc dantnmpdgv pqivsstvvt rdnsllndlt pdhvdthrrs
     1501 slpavpteta nednvnsnnn eppslpvplr ktsspqkrpr saststqvdp nhfetkrskq
     1561 gnpstrpmfs pgptrppfri pefkwsyihq rllsdvlfsl etdiqvwrsh stksvldfvn
     1621 ssenaifvvn tvhlisqlad nliiacggll pllasatspn seldvieptq gmpievavsf
     1681 lqrlvnmadv lifasslnfg eleaeknmss ggilrqclrl vctcavrncl eckergrpys
     1741 mlgrqigtsn ksqhiqslir gaqtspkniv dnlahqlspv kdpekllqdm dvnrlraviy
     1801 rdveetkqaq flslaivyfi svlmvskyrd ileppisqrp pspvqtiqtn gaglrpgsps
     1861 adgngggggr plfpqwshhv ypqflpgshp nanananana nhhmnqhhhq hplpaarhsn
     1921 rqppsnyylp nhhsnhynhh pnnhnyhrhh hyhhhnnnnh hhtlqkhidq prnlchrnsg
     1981 vglqgsggim sqagsqddge yeviivdenn ssiladhdvt ssgppstkgg hsgvprprti
     2041 ledytssept lgsstrtepl prniqsndss edtvhrsnit adpspsevtt dnenkhnsse
     2101 eawtdvnlne dgdtipmtnq redprnihnm thsrgdmqdn egnvldqeml gnaergekps
     2161 aeisvvrvpd rlvmtsaspr pddlpikglv dhlpvptpsr easltqklem algsvcpllr
     2221 eimvdfapfl sktlvgshgq ellmegkgrg rlttfknsns vvelvmllcs qewqnslqkh
     2281 aglafielin egrllshamk dhivrvanea efilnrmrad dvlkhadfes qcaqtlldrr
     2341 eeermcdhli taarrrdnvi asrllekvrn ilsnkhgawg ymdpmaakla eywkldawed
     2401 darrrkrfvh nplgsshpea tlkaalehga pedailqare efhahlaasr ahqqqlqsad
     2461 lmddsellsd drdldndltg pvnistkgkl iapgivapgi isvtstelyf evdeddpefk
     2521 kidsevlkyc dhlhgkwyfs evraifsrry llqnvaieif lasrtsilfa fpdqatvkkv
     2581 ikalprvgvg ikygiqqtrr asmmsprqlm rssnmtqkwq rreisnfeyl mflntiagrt
     2641 yndlnqypvf pwvltnyetk eldlslpsny rdlskpigal npsrrayfee rfqswehdsi
     2701 ppfhygthys taafvlnwmi rvepmttmfl alqggkfdhp nrlfssials wkncqrdtsd
     2761 vkelipefff lpemlvnsnr yrlgrqedgs svgdvelppw asspeefiri nrmalesefv
     2821 scqlhqwidl ifgykqkgpe avratnvfyy ltyegsvdld titdplmrea ienqirnfgq
     2881 tpsqllmeph pprssamhls takfdktlpp vlqspmmfss ipddvcmtik fpsnspichi
     2941 santypqlpl psvvtvttgq qfavnrwntn yaasvqspsy adtpqaqaan qpmsmdpvls
     3001 qaanssnpal rrhlgdnfsq kltirsncfv ttvdsrflva cgfwdnsfrv fstetakivq
     3061 ivfghygvvt clsrsecnit sdcyiasgsa dctvllwhwn artqtivgeg eapapratlt
     3121 gheqpvtavv isaelglvvs gsyygpvlvh ttfgdllrsl eapngfsspe niamsregvi
     3181 vvnyerghia aftingkrlr heshndnlqc lllsrdgeyl mtggdkrive vwrtfnlall
     3241 yafpacessv rslalshdqk fllaglangs ivifhidfnr whhefqqry
//
LOCUS       XP_034195679            3270 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X11 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195679
VERSION     XP_034195679.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339788.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3270
                     /product="A kinase anchor protein rugose isoform X11"
                     /calculated_mol_wt=366977
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..950
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1011..>1456
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2272..2437
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2469..2566
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2598..2874
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2913..3259
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3009..3045
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3051..3102
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3107..3142
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3148..3183
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3189..3224
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3270
                     /gene="rg"
                     /coded_by="XM_034339788.2:2375..12187"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlfyf sfltwqlldh
      601 vlfnpalwiy tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr
      661 aksgitpkgl dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede
      721 nlhdvlqmli slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst
      781 hkrkydvmsp hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy
      841 rlenpmilkv vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli
      901 amayihpknt eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq
      961 faqmyehyer qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv
     1021 ernykefdaa egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr
     1081 lsdkpetptt arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss
     1141 spdsmtvqev lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv
     1201 adntsdtaqa kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes
     1261 stdvvastes vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq
     1321 dnlsemkskv letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet
     1381 ceekvdveek kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn
     1441 vsdnqsdipv rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd
     1501 gvpqivsstv vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp
     1561 lrktsspqkr prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi
     1621 hqrllsdvlf sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg
     1681 llpllasats pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm
     1741 ssggilrqcl rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn
     1801 ivdnlahqls pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky
     1861 rdileppisq rppspvqtiq tngaglrpgs psadgngggg grplfpqwsh hvypqflpgs
     1921 hpnananana nanhhmnqhh hqhplpaarh snrqppsnyy lpnhhrsggi msqagsqddg
     1981 eyeviivden nssiladhdv tssgppstkg ghsgvprprt iledytssep tlgsstrtep
     2041 lprniqsnds sedtvhrsni tadpspsevt tdnenkhnss eeawtdvnln edgdtipmtn
     2101 qredprnihn mthsrgdmqd negnvldqem lgnaergekp saeisvvrvp drlvmtsasp
     2161 rpddlpikgl vdhlpvptps reasltqkle malgsvcpll reimvdfapf lsktlvgshg
     2221 qellmegkgr grlttfknsn svvelvmllc sqewqnslqk haglafieli negrllsham
     2281 kdhivrvane aefilnrmra ddvlkhadfe sqcaqtlldr reeermcdhl itaarrrdnv
     2341 iasrllekvr nilsnkhgaw gymdpmaakl aeywkldawe ddarrrkrfv hnplgsshpe
     2401 atlkaalehg apedailqar eefhahlaas rahqqqlqsa dlmddsells ddrdldndlt
     2461 gpvnistkgk liapgivapg iisvtstely fevdeddpef kkidsevlky cdhlhgkwyf
     2521 sevraifsrr yllqnvaiei flasrtsilf afpdqatvkk vikalprvgv gikygiqqtr
     2581 rasmmsprql mrssnmtqkw qrreisnfey lmflntiagr tyndlnqypv fpwvltnyet
     2641 keldlslpsn yrdlskpiga lnpsrrayfe erfqswehds ippfhygthy staafvlnwm
     2701 irvepmttmf lalqggkfdh pnrlfssial swkncqrdts dvkelipeff flpemlvnsn
     2761 ryrlgrqedg ssvgdvelpp wasspeefir inrmalesef vscqlhqwid lifgykqkgp
     2821 eavratnvfy yltyegsvdl dtitdplmre aienqirnfg qtpsqllmep hpprssamhl
     2881 stakfdktlp pvlqspmmfs sipddvcmti kfpsnspich isantypqlp lpsvvtvttg
     2941 qqfavnrwnt nyaasvqsps yadtpqaqaa nqpmsmdpvl sqaanssnpa lrrhlgdnfs
     3001 qkltirsncf vttvdsrflv acgfwdnsfr vfstetakiv qivfghygvv tclsrsecni
     3061 tsdcyiasgs adctvllwhw nartqtivge geapapratl tgheqpvtav visaelglvv
     3121 sgsyygpvlv httfgdllrs leapngfssp eniamsregv ivvnyerghi aaftingkrl
     3181 rheshndnlq clllsrdgey lmtggdkriv evwrtfnlal lyafpacess vrslalshdq
     3241 kfllaglang sivifhidfn rwhhefqqry
//
LOCUS       XP_034195680            3233 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X12 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195680
VERSION     XP_034195680.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339789.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3233
                     /product="A kinase anchor protein rugose isoform X12"
                     /calculated_mol_wt=362988
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..950
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1011..>1456
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2235..2400
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2432..2529
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2561..2837
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2876..3222
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          2972..3008
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3014..3065
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3070..3105
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3111..3146
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3152..3187
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3233
                     /gene="rg"
                     /coded_by="XM_034339789.2:2375..12076"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlfyf sfltwqlldh
      601 vlfnpalwiy tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr
      661 aksgitpkgl dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede
      721 nlhdvlqmli slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst
      781 hkrkydvmsp hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy
      841 rlenpmilkv vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli
      901 amayihpknt eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq
      961 faqmyehyer qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv
     1021 ernykefdaa egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr
     1081 lsdkpetptt arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss
     1141 spdsmtvqev lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv
     1201 adntsdtaqa kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes
     1261 stdvvastes vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq
     1321 dnlsemkskv letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet
     1381 ceekvdveek kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn
     1441 vsdnqsdipv rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd
     1501 gvpqivsstv vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp
     1561 lrktsspqkr prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi
     1621 hqrllsdvlf sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg
     1681 llpllasats pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm
     1741 ssggilrqcl rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn
     1801 ivdnlahqls pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky
     1861 rdileppisq rppspvqtiq tngaglrpgs psadgngggg grplfpqwsh hvypqflpgs
     1921 hpnananana nanhhmnqhh hqhplpaarh snrqppsnyy lpnhhrsggi msqagsqddg
     1981 eyeviivden nssiladhdv tssgppstks ndssedtvhr snitadpsps evttdnenkh
     2041 nsseeawtdv nlnedgdtip mtnqredprn ihnmthsrgd mqdnegnvld qemlgnaerg
     2101 ekpsaeisvv rvpdrlvmts asprpddlpi kglvdhlpvp tpsreasltq klemalgsvc
     2161 pllreimvdf apflsktlvg shgqellmeg kgrgrlttfk nsnsvvelvm llcsqewqns
     2221 lqkhaglafi elinegrlls hamkdhivrv aneaefilnr mraddvlkha dfesqcaqtl
     2281 ldrreeermc dhlitaarrr dnviasrlle kvrnilsnkh gawgymdpma aklaeywkld
     2341 aweddarrrk rfvhnplgss hpeatlkaal ehgapedail qareefhahl aasrahqqql
     2401 qsadlmddse llsddrdldn dltgpvnist kgkliapgiv apgiisvtst elyfevdedd
     2461 pefkkidsev lkycdhlhgk wyfsevraif srryllqnva ieiflasrts ilfafpdqat
     2521 vkkvikalpr vgvgikygiq qtrrasmmsp rqlmrssnmt qkwqrreisn feylmflnti
     2581 agrtyndlnq ypvfpwvltn yetkeldlsl psnyrdlskp igalnpsrra yfeerfqswe
     2641 hdsippfhyg thystaafvl nwmirvepmt tmflalqggk fdhpnrlfss ialswkncqr
     2701 dtsdvkelip effflpemlv nsnryrlgrq edgssvgdve lppwasspee firinrmale
     2761 sefvscqlhq widlifgykq kgpeavratn vfyyltyegs vdldtitdpl mreaienqir
     2821 nfgqtpsqll mephpprssa mhlstakfdk tlppvlqspm mfssipddvc mtikfpsnsp
     2881 ichisantyp qlplpsvvtv ttgqqfavnr wntnyaasvq spsyadtpqa qaanqpmsmd
     2941 pvlsqaanss npalrrhlgd nfsqkltirs ncfvttvdsr flvacgfwdn sfrvfsteta
     3001 kivqivfghy gvvtclsrse cnitsdcyia sgsadctvll whwnartqti vgegeapapr
     3061 atltgheqpv tavvisaelg lvvsgsyygp vlvhttfgdl lrsleapngf sspeniamsr
     3121 egvivvnyer ghiaafting krlrheshnd nlqclllsrd geylmtggdk rivevwrtfn
     3181 lallyafpac essvrslals hdqkfllagl angsivifhi dfnrwhhefq qry
//
LOCUS       XP_034195682            3197 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X13 [Osmia lignaria
            lignaria].
ACCESSION   XP_034195682
VERSION     XP_034195682.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339791.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3197
                     /product="A kinase anchor protein rugose isoform X13"
                     /calculated_mol_wt=358812
     Region          225..373
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          454..950
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <1011..1429
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2199..2364
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2396..2493
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2525..2801
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2840..3186
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          2936..2972
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          2978..3029
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3034..3069
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3075..3110
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3116..3151
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3197
                     /gene="rg"
                     /coded_by="XM_034339791.2:2373..11966"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 mdslerlmsa gshppaltaa trsqnafvqa nlcsvigrkg rhltgtfclt gdtmegiiqc
       61 lvflkafslv ggefdmelnf viqdaqnirh mlelldhcpa nlqaeiwsvf iailrksvrn
      121 lqactdvsli ehvlhrlsra etvvadllid mlgvlasysi tvkelkllfg amkavkgkwp
      181 rhsakllnvl rqmpqrngpd vffsfpgrkg saivlpplak wphengftft twfrldpins
      241 vnierekpyl ycfktskgvg ysahfvgncl vltsmkvkgk gfqhcvkyef qprkwymiav
      301 vyiynrwtks eikclvngql asstemawfv stndpfdkcy igatpevdee rvfcgqmsai
      361 ylfsealtth qicamhrlgp gyksqfrfdn ecylnlpdnh krvsdepela smvdqsmqtv
      421 lydgklsnai vfmynpvatd sqlclqsapk gnvsyfvhtp halmlqdvka vithsihstl
      481 nsiggiqvlf plfsqldmpy dciapndvkr dptlcskllg ficdlvessq tvqqhmvqnr
      541 gflvisymlq rasrdhltte vlasfleltk hlvtclsans dlllkqlfyf sfltwqlldh
      601 vlfnpalwiy tpapvqtrly sylateflsd tqiysnvrrv stvlqtvhtl kyyywvanpr
      661 aksgitpkgl dgprpqqkdi ltirsyillf lkqlimigng vkddelqsil nylttihede
      721 nlhdvlqmli slmsehpssm vpafdakqgv rtifkllaae sqlirlqalk llgfflsrst
      781 hkrkydvmsp hnlytllaer lllneetlsl ptynvlyeim tehisqqily vrhpepeshy
      841 rlenpmilkv vatlirqskq teqllevkkl flsdmtllcn nnrenrrtvl qmsvwqewli
      901 amayihpknt eeqkisdmvy slfrmllhha ikheyggwrv wvdtlaivhs kvsyeefklq
      961 faqmyehyer qrsdnitdpe lrqqrpisti sgwdqqhsgn ngynkpspwg nqqnhdvqhv
     1021 ernykefdaa egndrleesc scdlnsidnt esdgspaivk snsetldtpq sseaisldsr
     1081 lsdkpetptt arevhtdtpt ileegnseaa svpttqetse aisiesssdf ysevhkidss
     1141 spdsmtvqev lkkdeepseq keedtvsesa sakqteenvq aeqedavpee kstpkenanv
     1201 adntsdtaqa kedteasste tkdeetskek seaaddvpln ddvpsntevd sekaaatdes
     1261 stdvvastes vdsenipsas pgetpeplii kdieklqlen dreiidsdts eayltptenq
     1321 dnlsemkskv letekiednm vedaedtkst tvkdenkend neetvknpnc stsvientet
     1381 ceekvdveek kvesvvasse lssekcavds vtedesavin dndekviekh srepstistn
     1441 vsdnqsdipv rtetevevtd svcsnsdvqs svdnvtqvpn pkqqiptist vcdantnmpd
     1501 gvpqivsstv vtrdnsllnd ltpdhvdthr rsslpavpte tanednvnsn nneppslpvp
     1561 lrktsspqkr prsaststqv dpnhfetkrs kqgnpstrpm fspgptrppf ripefkwsyi
     1621 hqrllsdvlf sletdiqvwr shstksvldf vnssenaifv vntvhlisql adnliiacgg
     1681 llpllasats pnseldviep tqgmpievav sflqrlvnma dvlifassln fgeleaeknm
     1741 ssggilrqcl rlvctcavrn cleckergrp ysmlgrqigt snksqhiqsl irgaqtspkn
     1801 ivdnlahqls pvkdpekllq dmdvnrlrav iyrdveetkq aqflslaivy fisvlmvsky
     1861 rdileppisq rppspvqtiq tngaglrpgs psgsggimsq agsqddgeye viivdennss
     1921 iladhdvtss gppstkgghs gvprprtile dytsseptlg sstrteplpr niqsndssed
     1981 tvhrsnitad pspsevttdn enkhnsseea wtdvnlnedg dtipmtnqre dprnihnmth
     2041 srgdmqdneg nvldqemlgn aergekpsae isvvrvpdrl vmtsasprpd dlpikglvdh
     2101 lpvptpsrea sltqklemal gsvcpllrei mvdfapflsk tlvgshgqel lmegkgrgrl
     2161 ttfknsnsvv elvmllcsqe wqnslqkhag lafielineg rllshamkdh ivrvaneaef
     2221 ilnrmraddv lkhadfesqc aqtlldrree ermcdhlita arrrdnvias rllekvrnil
     2281 snkhgawgym dpmaaklaey wkldawedda rrrkrfvhnp lgsshpeatl kaalehgape
     2341 dailqareef hahlaasrah qqqlqsadlm ddsellsddr dldndltgpv nistkgklia
     2401 pgivapgiis vtstelyfev deddpefkki dsevlkycdh lhgkwyfsev raifsrryll
     2461 qnvaieifla srtsilfafp dqatvkkvik alprvgvgik ygiqqtrras mmsprqlmrs
     2521 snmtqkwqrr eisnfeylmf lntiagrtyn dlnqypvfpw vltnyetkel dlslpsnyrd
     2581 lskpigalnp srrayfeerf qswehdsipp fhygthysta afvlnwmirv epmttmflal
     2641 qggkfdhpnr lfssialswk ncqrdtsdvk elipeffflp emlvnsnryr lgrqedgssv
     2701 gdvelppwas speefirinr malesefvsc qlhqwidlif gykqkgpeav ratnvfyylt
     2761 yegsvdldti tdplmreaie nqirnfgqtp sqllmephpp rssamhlsta kfdktlppvl
     2821 qspmmfssip ddvcmtikfp snspichisa ntypqlplps vvtvttgqqf avnrwntnya
     2881 asvqspsyad tpqaqaanqp msmdpvlsqa anssnpalrr hlgdnfsqkl tirsncfvtt
     2941 vdsrflvacg fwdnsfrvfs tetakivqiv fghygvvtcl srsecnitsd cyiasgsadc
     3001 tvllwhwnar tqtivgegea papratltgh eqpvtavvis aelglvvsgs yygpvlvhtt
     3061 fgdllrslea pngfsspeni amsregvivv nyerghiaaf tingkrlrhe shndnlqcll
     3121 lsrdgeylmt ggdkrivevw rtfnlallya fpacessvrs lalshdqkfl laglangsiv
     3181 ifhidfnrwh hefqqry
//
LOCUS       XP_076546835            3291 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X14 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546835
VERSION     XP_076546835.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690720.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3291
                     /product="A kinase anchor protein rugose isoform X14"
                     /calculated_mol_wt=370093
     Region          210..358
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          439..935
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <996..>1441
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2307..2472
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2504..2601
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2633..2909
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2934..3280
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3030..3066
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3072..3123
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3128..3163
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3169..3204
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3210..3245
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3291
                     /gene="rg"
                     /coded_by="XM_076690720.1:211..10086"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 madmksppfs dikrpeevva mamndglkfa vliglievgq vsnrevvntv lhllvggefd
       61 melnfviqda qnirhmlell dhcpanlqae iwsvfiailr ksvrnlqact dvsliehvlh
      121 rlsraetvva dllidmlgvl asysitvkel kllfgamkav kgkwprhsak llnvlrqmpq
      181 rngpdvffsf pgrkgsaivl pplakwphen gftfttwfrl dpinsvnier ekpylycfkt
      241 skgvgysahf vgnclvltsm kvkgkgfqhc vkyefqprkw ymiavvyiyn rwtkseikcl
      301 vngqlasste mawfvstndp fdkcyigatp evdeervfcg qmsaiylfse altthqicam
      361 hrlgpgyksq frfdnecyln lpdnhkrvsd epelasmvdq smqtvlydgk lsnaivfmyn
      421 pvatdsqlcl qsapkgnvsy fvhtphalml qdvkaviths ihstlnsigg iqvlfplfsq
      481 ldmpydciap ndvkrdptlc skllgficdl vessqtvqqh mvqnrgflvi symlqrasrd
      541 hlttevlasf leltkhlvtc lsansdlllk qlfyfsfltw qlldhvlfnp alwiytpapv
      601 qtrlysylat eflsdtqiys nvrrvstvlq tvhtlkyyyw vanpraksgi tpkgldgprp
      661 qqkdiltirs yillflkqli migngvkdde lqsilnyltt ihedenlhdv lqmlislmse
      721 hpssmvpafd akqgvrtifk llaaesqlir lqalkllgff lsrsthkrky dvmsphnlyt
      781 llaerlllne etlslptynv lyeimtehis qqilyvrhpe peshyrlenp milkvvatli
      841 rqskqteqll evkklflsdm tllcnnnren rrtvlqmsvw qewliamayi hpknteeqki
      901 sdmvyslfrm llhhaikhey ggwrvwvdtl aivhskvsye efklqfaqmy ehyerqrsdn
      961 itdpelrqqr pistisgwdq qhsgnngynk pspwgnqqnh dvqhvernyk efdaaegndr
     1021 leescscdln sidntesdgs paivksnset ldtpqsseai sldsrlsdkp etpttarevh
     1081 tdtptileeg nseaasvptt qetseaisie sssdfysevh kidssspdsm tvqevlkkde
     1141 epseqkeedt vsesasakqt eenvqaeqed avpeekstpk enanvadnts dtaqakedte
     1201 asstetkdee tskekseaad dvplnddvps ntevdsekaa atdesstdvv astesvdsen
     1261 ipsaspgetp epliikdiek lqlendreii dsdtseaylt ptenqdnlse mkskvletek
     1321 iednmvedae dtksttvkde nkendneetv knpncstsvi entetceekv dveekkvesv
     1381 vasselssek cavdsvtede savindndek viekhsreps tistnvsdnq sdipvrtete
     1441 vevtdsvcsn sdvqssvdnv tqvpnpkqqi ptistvcdan tnmpdgvpqi vsstvvtrdn
     1501 sllndltpdh vdthrrsslp avptetaned nvnsnnnepp slpvplrkts spqkrprsas
     1561 tstqvdpnhf etkrskqgnp strpmfspgp trppfripef kwsyihqrll sdvlfsletd
     1621 iqvwrshstk svldfvnsse naifvvntvh lisqladnli iacggllpll asatspnsel
     1681 dvieptqgmp ievavsflqr lvnmadvlif asslnfgele aeknmssggi lrqclrlvct
     1741 cavrncleck ergrpysmlg rqigtsnksq hiqslirgaq tspknivdnl ahqlspvkdp
     1801 ekllqdmdvn rlraviyrdv eetkqaqfls laivyfisvl mvskyrdile ppisqrppsp
     1861 vqtiqtngag lrpgspsdgn gggggrplfp qwshhvypqf lpgshpnana nanananhhm
     1921 nqhhhqhplp aarhsnrqpp snyylpnhhs nhynhhpnnh nyhrhhhyhh hnnnnhhhtl
     1981 qkhidqprnl chrnsgvglq gsggimsqag sqddgeyevi ivdennssil adhdvtssgp
     2041 pstkgghsgv prprtiledy tsseptlgss trteplprni qsndssedtv hrsnitadps
     2101 psevttdnen khnsseeawt dvnlnedgdt ipmtnqredp rnihnmthsr gdmqdnegnv
     2161 ldqemlgnae rgekpsaeis vvrvpdrlvm tsasprpddl pikglvdhlp vptpsreasl
     2221 tqklemalgs vcpllreimv dfapflsktl vgshgqellm egkgrgrltt fknsnsvvel
     2281 vmllcsqewq nslqkhagla fielinegrl lshamkdhiv rvaneaefil nrmraddvlk
     2341 hadfesqcaq tlldrreeer mcdhlitaar rrdnviasrl lekvrnilsn khgawgymdp
     2401 maaklaeywk ldaweddarr rkrfvhnplg sshpeatlka alehgapeda ilqareefha
     2461 hlaasrahqq qlqsadlmdd sellsddrdl dndltgpvni stkgkliapg ivapgiisvt
     2521 stelyfevde ddpefkkids evlkycdhlh gkwyfsevra ifsrryllqn vaieiflasr
     2581 tsilfafpdq atvkkvikal prvgvgikyg iqqtrrasmm sprqlmrssn mtqkwqrrei
     2641 snfeylmfln tiagrtyndl nqypvfpwvl tnyetkeldl slpsnyrdls kpigalnpsr
     2701 rayfeerfqs wehdsippfh ygthystaaf vlnwmirvep mttmflalqg gkfdhpnrlf
     2761 ssialswknc qrdtsdvkel ipeffflpem lvnsnryrlg rqedgssvgd velppwassp
     2821 eefirinrma lesefvscql hqwidlifgy kqkgpeavra tnvfyyltye gsvdldtitd
     2881 plmreaienq irnfgqtpsq llmephpprs samhlspmmf ssipddvcmt ikfpsnspic
     2941 hisantypql plpsvvtvtt gqqfavnrwn tnyaasvqsp syadtpqaqa anqpmsmdpv
     3001 lsqaanssnp alrrhlgdnf sqkltirsnc fvttvdsrfl vacgfwdnsf rvfstetaki
     3061 vqivfghygv vtclsrsecn itsdcyiasg sadctvllwh wnartqtivg egeapaprat
     3121 ltgheqpvta vvisaelglv vsgsyygpvl vhttfgdllr sleapngfss peniamsreg
     3181 vivvnyergh iaaftingkr lrheshndnl qclllsrdge ylmtggdkri vevwrtfnla
     3241 llyafpaces svrslalshd qkfllaglan gsivifhidf nrwhhefqqr y
//
LOCUS       XP_076546836            3165 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X15 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546836
VERSION     XP_076546836.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690721.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3165
                     /product="A kinase anchor protein rugose isoform X15"
                     /calculated_mol_wt=355456
     Region          210..358
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          439..935
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <996..>1441
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2181..2346
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2378..2475
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2507..2783
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2808..3154
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          2904..2940
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          2946..2997
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3002..3037
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3043..3078
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3084..3119
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3165
                     /gene="rg"
                     /coded_by="XM_076690721.1:13..9510"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 madmksppfs dikrpeevva mamndglkfa vliglievgq vsnrevvntv lhllvggefd
       61 melnfviqda qnirhmlell dhcpanlqae iwsvfiailr ksvrnlqact dvsliehvlh
      121 rlsraetvva dllidmlgvl asysitvkel kllfgamkav kgkwprhsak llnvlrqmpq
      181 rngpdvffsf pgrkgsaivl pplakwphen gftfttwfrl dpinsvnier ekpylycfkt
      241 skgvgysahf vgnclvltsm kvkgkgfqhc vkyefqprkw ymiavvyiyn rwtkseikcl
      301 vngqlasste mawfvstndp fdkcyigatp evdeervfcg qmsaiylfse altthqicam
      361 hrlgpgyksq frfdnecyln lpdnhkrvsd epelasmvdq smqtvlydgk lsnaivfmyn
      421 pvatdsqlcl qsapkgnvsy fvhtphalml qdvkaviths ihstlnsigg iqvlfplfsq
      481 ldmpydciap ndvkrdptlc skllgficdl vessqtvqqh mvqnrgflvi symlqrasrd
      541 hlttevlasf leltkhlvtc lsansdlllk qlfyfsfltw qlldhvlfnp alwiytpapv
      601 qtrlysylat eflsdtqiys nvrrvstvlq tvhtlkyyyw vanpraksgi tpkgldgprp
      661 qqkdiltirs yillflkqli migngvkdde lqsilnyltt ihedenlhdv lqmlislmse
      721 hpssmvpafd akqgvrtifk llaaesqlir lqalkllgff lsrsthkrky dvmsphnlyt
      781 llaerlllne etlslptynv lyeimtehis qqilyvrhpe peshyrlenp milkvvatli
      841 rqskqteqll evkklflsdm tllcnnnren rrtvlqmsvw qewliamayi hpknteeqki
      901 sdmvyslfrm llhhaikhey ggwrvwvdtl aivhskvsye efklqfaqmy ehyerqrsdn
      961 itdpelrqqr pistisgwdq qhsgnngynk pspwgnqqnh dvqhvernyk efdaaegndr
     1021 leescscdln sidntesdgs paivksnset ldtpqsseai sldsrlsdkp etpttarevh
     1081 tdtptileeg nseaasvptt qetseaisie sssdfysevh kidssspdsm tvqevlkkde
     1141 epseqkeedt vsesasakqt eenvqaeqed avpeekstpk enanvadnts dtaqakedte
     1201 asstetkdee tskekseaad dvplnddvps ntevdsekaa atdesstdvv astesvdsen
     1261 ipsaspgetp epliikdiek lqlendreii dsdtseaylt ptenqdnlse mkskvletek
     1321 iednmvedae dtksttvkde nkendneetv knpncstsvi entetceekv dveekkvesv
     1381 vasselssek cavdsvtede savindndek viekhsreps tistnvsdnq sdipvrtete
     1441 vevtdsvcsn sdvqssvdnv tqvpnpkqqi ptistvcdan tnmpdgvpqi vsstvvtrdn
     1501 sllndltpdh vdthrrsslp avptetaned nvnsnnnepp slpvplrkts spqkrprsas
     1561 tstqvdpnhf etkrskqgnp strpmfspgp trppfripef kwsyihqrll sdvlfsletd
     1621 iqvwrshstk svldfvnsse naifvvntvh lisqladnli iacggllpll asatspnsel
     1681 dvieptqgmp ievavsflqr lvnmadvlif asslnfgele aeknmssggi lrqclrlvct
     1741 cavrncleck ergrpysmlg rqigtsnksq hiqslirgaq tspknivdnl ahqlspvkdp
     1801 ekllqdmdvn rlraviyrdv eetkqaqfls laivyfisvl mvskyrdile ppisqrppsp
     1861 vqtiqtngag lrpgspsgsg gimsqagsqd dgeyeviivd ennssiladh dvtssgppst
     1921 kgghsgvprp rtiledytss eptlgsstrt eplprniqsn dssedtvhrs nitadpspse
     1981 vttdnenkhn sseeawtdvn lnedgdtipm tnqredprni hnmthsrgdm qdnegnvldq
     2041 emlgnaerge kpsaeisvvr vpdrlvmtsa sprpddlpik glvdhlpvpt psreasltqk
     2101 lemalgsvcp llreimvdfa pflsktlvgs hgqellmegk glttfknsns vvelvmllcs
     2161 qewqnslqkh aglafielin egrllshamk dhivrvanea efilnrmrad dvlkhadfes
     2221 qcaqtlldrr eeermcdhli taarrrdnvi asrllekvrn ilsnkhgawg ymdpmaakla
     2281 eywkldawed darrrkrfvh nplgsshpea tlkaalehga pedailqare efhahlaasr
     2341 ahqqqlqsad lmddsellsd drdldndltg pvnistkgkl iapgivapgi isvtstelyf
     2401 evdeddpefk kidsevlkyc dhlhgkwyfs evraifsrry llqnvaieif lasrtsilfa
     2461 fpdqatvkkv ikalprvgvg ikygiqqtrr asmmsprqlm rssnmtqkwq rreisnfeyl
     2521 mflntiagrt yndlnqypvf pwvltnyetk eldlslpsny rdlskpigal npsrrayfee
     2581 rfqswehdsi ppfhygthys taafvlnwmi rvepmttmfl alqggkfdhp nrlfssials
     2641 wkncqrdtsd vkelipefff lpemlvnsnr yrlgrqedgs svgdvelppw asspeefiri
     2701 nrmalesefv scqlhqwidl ifgykqkgpe avratnvfyy ltyegsvdld titdplmrea
     2761 ienqirnfgq tpsqllmeph pprssamhls pmmfssipdd vcmtikfpsn spichisant
     2821 ypqlplpsvv tvttgqqfav nrwntnyaas vqspsyadtp qaqaanqpms mdpvlsqaan
     2881 ssnpalrrhl gdnfsqklti rsncfvttvd srflvacgfw dnsfrvfste takivqivfg
     2941 hygvvtclsr secnitsdcy iasgsadctv llwhwnartq tivgegeapa pratltgheq
     3001 pvtavvisae lglvvsgsyy gpvlvhttfg dllrsleapn gfsspeniam sregvivvny
     3061 erghiaafti ngkrlrhesh ndnlqcllls rdgeylmtgg dkrivevwrt fnlallyafp
     3121 acessvrsla lshdqkflla glangsivif hidfnrwhhe fqqry
//
LOCUS       XP_076546837            3288 aa            linear   INV 03-SEP-2025
DEFINITION  A kinase anchor protein rugose isoform X16 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546837
VERSION     XP_076546837.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690722.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3288
                     /product="A kinase anchor protein rugose isoform X16"
                     /calculated_mol_wt=369724
     Region          210..358
                     /region_name="Laminin_G_3"
                     /note="Concanavalin A-like lectin/glucanases superfamily;
                     pfam13385"
                     /db_xref="CDD:463865"
     Region          439..935
                     /region_name="DUF4704"
                     /note="Neurobeachin/BDCP, DUF4704 alpha solenoid region;
                     pfam15787"
                     /db_xref="CDD:464870"
     Region          <996..>1441
                     /region_name="MDN1"
                     /note="Midasin, AAA ATPase with vWA domain, involved in
                     ribosome maturation [Translation, ribosomal structure and
                     biogenesis]; COG5271"
                     /db_xref="CDD:444083"
     Region          2304..2469
                     /region_name="DUF1088"
                     /note="Neurobeachin-like, DUF1088; pfam06469"
                     /db_xref="CDD:461925"
     Region          2501..2598
                     /region_name="PH_BEACH"
                     /note="PH domain associated with Beige/BEACH; pfam14844"
                     /db_xref="CDD:434260"
     Region          2630..2906
                     /region_name="Beach"
                     /note="Beige/BEACH domain; pfam02138"
                     /db_xref="CDD:460459"
     Region          2931..3277
                     /region_name="NBCH_WD40"
                     /note="Neurobeachin beta propeller domain; pfam20426"
                     /db_xref="CDD:466575"
     Region          3027..3063
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3069..3120
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3125..3160
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3166..3201
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          3207..3242
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..3288
                     /gene="rg"
                     /coded_by="XM_076690722.1:211..10077"
                     /db_xref="GeneID:117611697"
ORIGIN      
        1 madmksppfs dikrpeevva mamndglkfa vliglievgq vsnrevvntv lhllvggefd
       61 melnfviqda qnirhmlell dhcpanlqae iwsvfiailr ksvrnlqact dvsliehvlh
      121 rlsraetvva dllidmlgvl asysitvkel kllfgamkav kgkwprhsak llnvlrqmpq
      181 rngpdvffsf pgrkgsaivl pplakwphen gftfttwfrl dpinsvnier ekpylycfkt
      241 skgvgysahf vgnclvltsm kvkgkgfqhc vkyefqprkw ymiavvyiyn rwtkseikcl
      301 vngqlasste mawfvstndp fdkcyigatp evdeervfcg qmsaiylfse altthqicam
      361 hrlgpgyksq frfdnecyln lpdnhkrvsd epelasmvdq smqtvlydgk lsnaivfmyn
      421 pvatdsqlcl qsapkgnvsy fvhtphalml qdvkaviths ihstlnsigg iqvlfplfsq
      481 ldmpydciap ndvkrdptlc skllgficdl vessqtvqqh mvqnrgflvi symlqrasrd
      541 hlttevlasf leltkhlvtc lsansdlllk qlfyfsfltw qlldhvlfnp alwiytpapv
      601 qtrlysylat eflsdtqiys nvrrvstvlq tvhtlkyyyw vanpraksgi tpkgldgprp
      661 qqkdiltirs yillflkqli migngvkdde lqsilnyltt ihedenlhdv lqmlislmse
      721 hpssmvpafd akqgvrtifk llaaesqlir lqalkllgff lsrsthkrky dvmsphnlyt
      781 llaerlllne etlslptynv lyeimtehis qqilyvrhpe peshyrlenp milkvvatli
      841 rqskqteqll evkklflsdm tllcnnnren rrtvlqmsvw qewliamayi hpknteeqki
      901 sdmvyslfrm llhhaikhey ggwrvwvdtl aivhskvsye efklqfaqmy ehyerqrsdn
      961 itdpelrqqr pistisgwdq qhsgnngynk pspwgnqqnh dvqhvernyk efdaaegndr
     1021 leescscdln sidntesdgs paivksnset ldtpqsseai sldsrlsdkp etpttarevh
     1081 tdtptileeg nseaasvptt qetseaisie sssdfysevh kidssspdsm tvqevlkkde
     1141 epseqkeedt vsesasakqt eenvqaeqed avpeekstpk enanvadnts dtaqakedte
     1201 asstetkdee tskekseaad dvplnddvps ntevdsekaa atdesstdvv astesvdsen
     1261 ipsaspgetp epliikdiek lqlendreii dsdtseaylt ptenqdnlse mkskvletek
     1321 iednmvedae dtksttvkde nkendneetv knpncstsvi entetceekv dveekkvesv
     1381 vasselssek cavdsvtede savindndek viekhsreps tistnvsdnq sdipvrtete
     1441 vevtdsvcsn sdvqssvdnv tqvpnpkqqi ptistvcdan tnmpdgvpqi vsstvvtrdn
     1501 sllndltpdh vdthrrsslp avptetaned nvnsnnnepp slpvplrkts spqkrprsas
     1561 tstqvdpnhf etkrskqgnp strpmfspgp trppfripef kwsyihqrll sdvlfsletd
     1621 iqvwrshstk svldfvnsse naifvvntvh lisqladnli iacggllpll asatspnsel
     1681 dvieptqgmp ievavsflqr lvnmadvlif asslnfgele aeknmssggi lrqclrlvct
     1741 cavrncleck ergrpysmlg rqigtsnksq hiqslirgaq tspknivdnl ahqlspvkdp
     1801 ekllqdmdvn rlraviyrdv eetkqaqfls laivyfisvl mvskyrdile ppisqrppsp
     1861 vqtiqtngag lrpgspsdgn gggggrplfp qwshhvypqf lpgshpnana nanananhhm
     1921 nqhhhqhplp aarhsnrqpp snyylpnhhs nhynhhpnnh nyhrhhhyhh hnnnnhhhtl
     1981 qkhidqprnl chrnsgvglq gsggimsqag sqddgeyevi ivdennssil adhdvtssgp
     2041 pstkgghsgv prprtiledy tsseptlgss trteplprni qsndssedtv hrsnitadps
     2101 psevttdnen khnsseeawt dvnlnedgdt ipmtnqredp rnihnmthsr gdmqdnegnv
     2161 ldqemlgnae rgekpsaeis vvrvpdrlvm tsasprpddl pikglvdhlp vptpsreasl
     2221 tqklemalgs vcpllreimv dfapflsktl vgshgqellm egkglttfkn snsvvelvml
     2281 lcsqewqnsl qkhaglafie linegrllsh amkdhivrva neaefilnrm raddvlkhad
     2341 fesqcaqtll drreeermcd hlitaarrrd nviasrllek vrnilsnkhg awgymdpmaa
     2401 klaeywklda weddarrrkr fvhnplgssh peatlkaale hgapedailq areefhahla
     2461 asrahqqqlq sadlmddsel lsddrdldnd ltgpvnistk gkliapgiva pgiisvtste
     2521 lyfevdeddp efkkidsevl kycdhlhgkw yfsevraifs rryllqnvai eiflasrtsi
     2581 lfafpdqatv kkvikalprv gvgikygiqq trrasmmspr qlmrssnmtq kwqrreisnf
     2641 eylmflntia grtyndlnqy pvfpwvltny etkeldlslp snyrdlskpi galnpsrray
     2701 feerfqsweh dsippfhygt hystaafvln wmirvepmtt mflalqggkf dhpnrlfssi
     2761 alswkncqrd tsdvkelipe ffflpemlvn snryrlgrqe dgssvgdvel ppwasspeef
     2821 irinrmales efvscqlhqw idlifgykqk gpeavratnv fyyltyegsv dldtitdplm
     2881 reaienqirn fgqtpsqllm ephpprssam hlspmmfssi pddvcmtikf psnspichis
     2941 antypqlplp svvtvttgqq favnrwntny aasvqspsya dtpqaqaanq pmsmdpvlsq
     3001 aanssnpalr rhlgdnfsqk ltirsncfvt tvdsrflvac gfwdnsfrvf stetakivqi
     3061 vfghygvvtc lsrsecnits dcyiasgsad ctvllwhwna rtqtivgege apapratltg
     3121 heqpvtavvi saelglvvsg syygpvlvht tfgdllrsle apngfsspen iamsregviv
     3181 vnyerghiaa ftingkrlrh eshndnlqcl llsrdgeylm tggdkrivev wrtfnlally
     3241 afpacessvr slalshdqkf llaglangsi vifhidfnrw hhefqqry
//
LOCUS       XP_034195714             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195714
VERSION     XP_034195714.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339823.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339823.2:1122..3380"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195715             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195715
VERSION     XP_034195715.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339824.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339824.2:1051..3309"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195716             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195716
VERSION     XP_034195716.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339825.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339825.2:827..3085"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195717             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195717
VERSION     XP_034195717.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339826.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339826.2:1118..3376"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_076546838             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_076546838
VERSION     XP_076546838.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690723.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_076690723.1:977..3235"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195719             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195719
VERSION     XP_034195719.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339828.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339828.2:743..3001"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195721             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195721
VERSION     XP_034195721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339830.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339830.2:975..3233"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_076546839             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_076546839
VERSION     XP_076546839.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690724.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_076690724.1:973..3231"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195722             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195722
VERSION     XP_034195722.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339831.2:173..2431"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195724             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195724
VERSION     XP_034195724.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339833.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339833.2:173..2431"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_034195725             752 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat, immunoglobulin-like domain-containing kekkon 5
            protein [Osmia lignaria lignaria].
ACCESSION   XP_034195725
VERSION     XP_034195725.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339834.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..752
                     /product="leucine-rich repeat, immunoglobulin-like
                     domain-containing kekkon 5 protein"
                     /calculated_mol_wt=82477
     Region          <46..>236
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          65..87
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          88..111
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          112..135
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          136..159
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          160..183
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          184..207
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          216..264
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     Region          282..361
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          284..288
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..301
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          319..333
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          343..348
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          356..359
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          519..>704
                     /region_name="PHA03307"
                     /note="transcriptional regulator ICP4; Provisional"
                     /db_xref="CDD:223039"
     CDS             1..752
                     /gene="kek5"
                     /coded_by="XM_034339834.2:247..2505"
                     /db_xref="GeneID:117611702"
ORIGIN      
        1 melrtaklll ysalvvvvcw cqedwtaqcs slckcrwisg kktaecakqn ltqipsnlss
       61 diqnfdltgn riahlvnnsf ssvhlvnlqk lvlrkceiet vhmnafnglk ivieidlsan
      121 nirtlnrgtf eetqrlrvll lnenklkvle nglfrdlvyl qkvmlsnnel eriekktfhn
      181 lpglrlvtld gnnlstlgvq sfellpilgs lelhnnpwnc nchlkrfrnw tierklytkp
      241 ttcqqppnla gkmwdeissd efacrpeift igpsvkmefa rgnvtfwcra sgtpqpklfw
      301 iyrsrtltne srrhngeksy ilksshdwln ltipdvtpsd kgdyvclaks pggnteknvt
      361 laiagdamgg kdniislpla lglgvtalll livtvslcvc ycrrrrtrhd eksleaatme
      421 hhglgeqeks littinpvvk pprryetpsv tshgtemtel nrtlldndsv fadgigggvg
      481 sgviggvgdd ereeratpel esgtgtlcrg gggsyrqypp dllafsggrg asptsqasta
      541 pdntrlpsqh atpnsaaygs psnqypatfk tlphnrsvtp ygitsstiap vmprhgyvti
      601 prrprapsws sgpptsptdg lepvydnlgl rttadgssvl slnkspepls sirnrplpgt
      661 psshygtiqr stpniltssp ldraapegaa ewpskiades tdsnhvlnqs qpqqsssntl
      721 grkvpprppp kpkkkstngp lyedegedgt ev
//
LOCUS       XP_076546840             711 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611703 [Osmia lignaria lignaria].
ACCESSION   XP_076546840
VERSION     XP_076546840.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690725.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..711
                     /product="uncharacterized protein LOC117611703"
                     /calculated_mol_wt=77720
     Region          465..652
                     /region_name="PTZ00062"
                     /note="glutaredoxin; Provisional"
                     /db_xref="CDD:240250"
     Region          563..708
                     /region_name="GRX_GRX_like"
                     /note="Glutaredoxin (GRX) family, GRX-like domain
                     containing protein subfamily; composed of uncharacterized
                     eukaryotic proteins containing a GRX-like domain having
                     only one conserved cysteine, aligning to the C-terminal
                     cysteine of the CXXC motif of GRXs. This...; cd03031"
                     /db_xref="CDD:239329"
     Site            order(569,578..580,621)
                     /site_type="other"
                     /note="putative GSH binding site [chemical binding]"
                     /db_xref="CDD:239329"
     Site            581
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239329"
     CDS             1..711
                     /gene="LOC117611703"
                     /coded_by="XM_076690725.1:1052..3187"
                     /db_xref="GeneID:117611703"
ORIGIN      
        1 mdeivtmtvp ptlpaktqpr dpsqvarqtv sirtvsnppt aplstgagat vfvglaapgv
       61 dsaaacrrri pevqsangyg kqstvksaqn lpsqqqqqqq qqqaahvvki kinpdgekng
      121 rvistvrltl dekivgqeae qengngigia cehsskrdgc vvvsaanlvn eqrceragag
      181 egcvrisvac ntfernnnnn nnkdkrnrkd ssekdmvhre sseplntssa nnrstsacfy
      241 ynsypshlng mvmssgqcsp sdtldsgtcs dldgtppplp kkknsstvml asdqhnrtgs
      301 ltssgaevds ddnesniscd slnagdlndr ieeingnsng vnrssldfrg hsennhrqky
      361 rdmndvpnln qlnlsnekee rtsketvttp eiqsnscstr aspsvspsps atsslskass
      421 tprirspvlt sesngrlssp vvkectyeer kqeqerigqe pvggevdasi npvtgkkyly
      481 eydrfykfhv nelkgnskdt nrgvvlgdkd tdecfagyki ldkeairsak gtvrgvknrv
      541 ragiatflqk pssqayikke lgkvvvyttt sgivrktfyn ckkvkqilrt hmvkydeldl
      601 fgdaelqtel rerlgsmviq lpqlfidgqh iggfdtverl nesgelrdml kpyqsedact
      661 vclfcggyqw qlcpvcngsk rsvhrndfta efvalkcakc dvnglircph c
//
LOCUS       XP_034195726             711 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611703 [Osmia lignaria lignaria].
ACCESSION   XP_034195726
VERSION     XP_034195726.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339835.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..711
                     /product="uncharacterized protein LOC117611703"
                     /calculated_mol_wt=77720
     Region          465..652
                     /region_name="PTZ00062"
                     /note="glutaredoxin; Provisional"
                     /db_xref="CDD:240250"
     Region          563..708
                     /region_name="GRX_GRX_like"
                     /note="Glutaredoxin (GRX) family, GRX-like domain
                     containing protein subfamily; composed of uncharacterized
                     eukaryotic proteins containing a GRX-like domain having
                     only one conserved cysteine, aligning to the C-terminal
                     cysteine of the CXXC motif of GRXs. This...; cd03031"
                     /db_xref="CDD:239329"
     Site            order(569,578..580,621)
                     /site_type="other"
                     /note="putative GSH binding site [chemical binding]"
                     /db_xref="CDD:239329"
     Site            581
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239329"
     CDS             1..711
                     /gene="LOC117611703"
                     /coded_by="XM_034339835.2:804..2939"
                     /db_xref="GeneID:117611703"
ORIGIN      
        1 mdeivtmtvp ptlpaktqpr dpsqvarqtv sirtvsnppt aplstgagat vfvglaapgv
       61 dsaaacrrri pevqsangyg kqstvksaqn lpsqqqqqqq qqqaahvvki kinpdgekng
      121 rvistvrltl dekivgqeae qengngigia cehsskrdgc vvvsaanlvn eqrceragag
      181 egcvrisvac ntfernnnnn nnkdkrnrkd ssekdmvhre sseplntssa nnrstsacfy
      241 ynsypshlng mvmssgqcsp sdtldsgtcs dldgtppplp kkknsstvml asdqhnrtgs
      301 ltssgaevds ddnesniscd slnagdlndr ieeingnsng vnrssldfrg hsennhrqky
      361 rdmndvpnln qlnlsnekee rtsketvttp eiqsnscstr aspsvspsps atsslskass
      421 tprirspvlt sesngrlssp vvkectyeer kqeqerigqe pvggevdasi npvtgkkyly
      481 eydrfykfhv nelkgnskdt nrgvvlgdkd tdecfagyki ldkeairsak gtvrgvknrv
      541 ragiatflqk pssqayikke lgkvvvyttt sgivrktfyn ckkvkqilrt hmvkydeldl
      601 fgdaelqtel rerlgsmviq lpqlfidgqh iggfdtverl nesgelrdml kpyqsedact
      661 vclfcggyqw qlcpvcngsk rsvhrndfta efvalkcakc dvnglircph c
//
LOCUS       XP_034195727             711 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611703 [Osmia lignaria lignaria].
ACCESSION   XP_034195727
VERSION     XP_034195727.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339836.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..711
                     /product="uncharacterized protein LOC117611703"
                     /calculated_mol_wt=77720
     Region          465..652
                     /region_name="PTZ00062"
                     /note="glutaredoxin; Provisional"
                     /db_xref="CDD:240250"
     Region          563..708
                     /region_name="GRX_GRX_like"
                     /note="Glutaredoxin (GRX) family, GRX-like domain
                     containing protein subfamily; composed of uncharacterized
                     eukaryotic proteins containing a GRX-like domain having
                     only one conserved cysteine, aligning to the C-terminal
                     cysteine of the CXXC motif of GRXs. This...; cd03031"
                     /db_xref="CDD:239329"
     Site            order(569,578..580,621)
                     /site_type="other"
                     /note="putative GSH binding site [chemical binding]"
                     /db_xref="CDD:239329"
     Site            581
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239329"
     CDS             1..711
                     /gene="LOC117611703"
                     /coded_by="XM_034339836.2:481..2616"
                     /db_xref="GeneID:117611703"
ORIGIN      
        1 mdeivtmtvp ptlpaktqpr dpsqvarqtv sirtvsnppt aplstgagat vfvglaapgv
       61 dsaaacrrri pevqsangyg kqstvksaqn lpsqqqqqqq qqqaahvvki kinpdgekng
      121 rvistvrltl dekivgqeae qengngigia cehsskrdgc vvvsaanlvn eqrceragag
      181 egcvrisvac ntfernnnnn nnkdkrnrkd ssekdmvhre sseplntssa nnrstsacfy
      241 ynsypshlng mvmssgqcsp sdtldsgtcs dldgtppplp kkknsstvml asdqhnrtgs
      301 ltssgaevds ddnesniscd slnagdlndr ieeingnsng vnrssldfrg hsennhrqky
      361 rdmndvpnln qlnlsnekee rtsketvttp eiqsnscstr aspsvspsps atsslskass
      421 tprirspvlt sesngrlssp vvkectyeer kqeqerigqe pvggevdasi npvtgkkyly
      481 eydrfykfhv nelkgnskdt nrgvvlgdkd tdecfagyki ldkeairsak gtvrgvknrv
      541 ragiatflqk pssqayikke lgkvvvyttt sgivrktfyn ckkvkqilrt hmvkydeldl
      601 fgdaelqtel rerlgsmviq lpqlfidgqh iggfdtverl nesgelrdml kpyqsedact
      661 vclfcggyqw qlcpvcngsk rsvhrndfta efvalkcakc dvnglircph c
//
LOCUS       XP_034195728             711 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611703 [Osmia lignaria lignaria].
ACCESSION   XP_034195728
VERSION     XP_034195728.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339837.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..711
                     /product="uncharacterized protein LOC117611703"
                     /calculated_mol_wt=77720
     Region          465..652
                     /region_name="PTZ00062"
                     /note="glutaredoxin; Provisional"
                     /db_xref="CDD:240250"
     Region          563..708
                     /region_name="GRX_GRX_like"
                     /note="Glutaredoxin (GRX) family, GRX-like domain
                     containing protein subfamily; composed of uncharacterized
                     eukaryotic proteins containing a GRX-like domain having
                     only one conserved cysteine, aligning to the C-terminal
                     cysteine of the CXXC motif of GRXs. This...; cd03031"
                     /db_xref="CDD:239329"
     Site            order(569,578..580,621)
                     /site_type="other"
                     /note="putative GSH binding site [chemical binding]"
                     /db_xref="CDD:239329"
     Site            581
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239329"
     CDS             1..711
                     /gene="LOC117611703"
                     /coded_by="XM_034339837.2:419..2554"
                     /db_xref="GeneID:117611703"
ORIGIN      
        1 mdeivtmtvp ptlpaktqpr dpsqvarqtv sirtvsnppt aplstgagat vfvglaapgv
       61 dsaaacrrri pevqsangyg kqstvksaqn lpsqqqqqqq qqqaahvvki kinpdgekng
      121 rvistvrltl dekivgqeae qengngigia cehsskrdgc vvvsaanlvn eqrceragag
      181 egcvrisvac ntfernnnnn nnkdkrnrkd ssekdmvhre sseplntssa nnrstsacfy
      241 ynsypshlng mvmssgqcsp sdtldsgtcs dldgtppplp kkknsstvml asdqhnrtgs
      301 ltssgaevds ddnesniscd slnagdlndr ieeingnsng vnrssldfrg hsennhrqky
      361 rdmndvpnln qlnlsnekee rtsketvttp eiqsnscstr aspsvspsps atsslskass
      421 tprirspvlt sesngrlssp vvkectyeer kqeqerigqe pvggevdasi npvtgkkyly
      481 eydrfykfhv nelkgnskdt nrgvvlgdkd tdecfagyki ldkeairsak gtvrgvknrv
      541 ragiatflqk pssqayikke lgkvvvyttt sgivrktfyn ckkvkqilrt hmvkydeldl
      601 fgdaelqtel rerlgsmviq lpqlfidgqh iggfdtverl nesgelrdml kpyqsedact
      661 vclfcggyqw qlcpvcngsk rsvhrndfta efvalkcakc dvnglircph c
//
LOCUS       XP_034195730             684 aa            linear   INV 03-SEP-2025
DEFINITION  xylosyl- and glucuronyltransferase LARGE1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195730
VERSION     XP_034195730.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339839.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195730.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="xylosyl- and glucuronyltransferase LARGE1
                     isoform X1"
                     /calculated_mol_wt=79919
     Region          67..344
                     /region_name="GT8_LARGE_C"
                     /note="LARGE catalytic domain has closest homology to GT8
                     glycosyltransferase involved in lipooligosaccharide
                     synthesis; cd06431"
                     /db_xref="CDD:133053"
     Site            order(72..74,77..78,153,167,169..171,199,223..225,
                     261..262,287..288,307,309..310,313)
                     /site_type="active"
                     /note="putative ligand binding site [active]"
                     /db_xref="CDD:133053"
     Site            order(169,171,307)
                     /site_type="active"
                     /note="metal binding site [active]"
                     /db_xref="CDD:133053"
     Region          390..661
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..684
                     /gene="LOC117611705"
                     /coded_by="XM_034339839.2:469..2523"
                     /db_xref="GeneID:117611705"
ORIGIN      
        1 marswlgclf gvlvpsaify lylllnvpsa didsfkivgk eslhverpif dklhsetnrn
       61 eakcetihia mvcagynstf alvtvvksvl fyrtkplhfh llvdeiakrt lttvfrtwdl
      121 phvnltyyka erwvpkvswi pnkhysgvyg llklilpdam redkvlvfdt dvtvvndvnl
      181 lwqmfekftn dqalgltenq shwyikalsy gqrpwpalgr gyntgvmlmh lqqlrnrkft
      241 slwetvtkrv layipetsla dqdimnavik dhpsivykie ctwniqlsdh tisdqcyrdt
      301 srinivhwns prkqdvynkh inefrklhki fldmdgnllr rrlfgcdkhe avseynessp
      361 creftrgatt lyrthpflle yeynvyapad valitqcsve riplledisk hwpgtisval
      421 yltdaelqnf lefvrgsvel rkrkniayhv vykdgelypi nylrniamsy vsspfifqld
      481 vdflpqyglh ealisyivkl nisesdkval ivpafetery rftfpankde llkflkrgvl
      541 ytfryhvwtq ghaatnysyw rntmepyevs wepdfepyiv vsklaprydt rfigfgwnkv
      601 sylthltvlg ykyivlpdtf iihrphapsl digkfrtdsi yrrclkklkd dfveelvtky
      661 gesalsklkk vtkeekilkp vktk
//
LOCUS       XP_034195731             684 aa            linear   INV 03-SEP-2025
DEFINITION  xylosyl- and glucuronyltransferase LARGE1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195731
VERSION     XP_034195731.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339840.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195731.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="xylosyl- and glucuronyltransferase LARGE1
                     isoform X1"
                     /calculated_mol_wt=79919
     Region          67..344
                     /region_name="GT8_LARGE_C"
                     /note="LARGE catalytic domain has closest homology to GT8
                     glycosyltransferase involved in lipooligosaccharide
                     synthesis; cd06431"
                     /db_xref="CDD:133053"
     Site            order(72..74,77..78,153,167,169..171,199,223..225,
                     261..262,287..288,307,309..310,313)
                     /site_type="active"
                     /note="putative ligand binding site [active]"
                     /db_xref="CDD:133053"
     Site            order(169,171,307)
                     /site_type="active"
                     /note="metal binding site [active]"
                     /db_xref="CDD:133053"
     Region          390..661
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..684
                     /gene="LOC117611705"
                     /coded_by="XM_034339840.2:408..2462"
                     /db_xref="GeneID:117611705"
ORIGIN      
        1 marswlgclf gvlvpsaify lylllnvpsa didsfkivgk eslhverpif dklhsetnrn
       61 eakcetihia mvcagynstf alvtvvksvl fyrtkplhfh llvdeiakrt lttvfrtwdl
      121 phvnltyyka erwvpkvswi pnkhysgvyg llklilpdam redkvlvfdt dvtvvndvnl
      181 lwqmfekftn dqalgltenq shwyikalsy gqrpwpalgr gyntgvmlmh lqqlrnrkft
      241 slwetvtkrv layipetsla dqdimnavik dhpsivykie ctwniqlsdh tisdqcyrdt
      301 srinivhwns prkqdvynkh inefrklhki fldmdgnllr rrlfgcdkhe avseynessp
      361 creftrgatt lyrthpflle yeynvyapad valitqcsve riplledisk hwpgtisval
      421 yltdaelqnf lefvrgsvel rkrkniayhv vykdgelypi nylrniamsy vsspfifqld
      481 vdflpqyglh ealisyivkl nisesdkval ivpafetery rftfpankde llkflkrgvl
      541 ytfryhvwtq ghaatnysyw rntmepyevs wepdfepyiv vsklaprydt rfigfgwnkv
      601 sylthltvlg ykyivlpdtf iihrphapsl digkfrtdsi yrrclkklkd dfveelvtky
      661 gesalsklkk vtkeekilkp vktk
//
LOCUS       XP_076546841             684 aa            linear   INV 03-SEP-2025
DEFINITION  xylosyl- and glucuronyltransferase LARGE1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546841
VERSION     XP_076546841.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690726.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="xylosyl- and glucuronyltransferase LARGE1
                     isoform X1"
                     /calculated_mol_wt=79919
     Region          67..344
                     /region_name="GT8_LARGE_C"
                     /note="LARGE catalytic domain has closest homology to GT8
                     glycosyltransferase involved in lipooligosaccharide
                     synthesis; cd06431"
                     /db_xref="CDD:133053"
     Site            order(72..74,77..78,153,167,169..171,199,223..225,
                     261..262,287..288,307,309..310,313)
                     /site_type="active"
                     /note="putative ligand binding site [active]"
                     /db_xref="CDD:133053"
     Site            order(169,171,307)
                     /site_type="active"
                     /note="metal binding site [active]"
                     /db_xref="CDD:133053"
     Region          390..661
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..684
                     /gene="LOC117611705"
                     /coded_by="XM_076690726.1:162..2216"
                     /db_xref="GeneID:117611705"
ORIGIN      
        1 marswlgclf gvlvpsaify lylllnvpsa didsfkivgk eslhverpif dklhsetnrn
       61 eakcetihia mvcagynstf alvtvvksvl fyrtkplhfh llvdeiakrt lttvfrtwdl
      121 phvnltyyka erwvpkvswi pnkhysgvyg llklilpdam redkvlvfdt dvtvvndvnl
      181 lwqmfekftn dqalgltenq shwyikalsy gqrpwpalgr gyntgvmlmh lqqlrnrkft
      241 slwetvtkrv layipetsla dqdimnavik dhpsivykie ctwniqlsdh tisdqcyrdt
      301 srinivhwns prkqdvynkh inefrklhki fldmdgnllr rrlfgcdkhe avseynessp
      361 creftrgatt lyrthpflle yeynvyapad valitqcsve riplledisk hwpgtisval
      421 yltdaelqnf lefvrgsvel rkrkniayhv vykdgelypi nylrniamsy vsspfifqld
      481 vdflpqyglh ealisyivkl nisesdkval ivpafetery rftfpankde llkflkrgvl
      541 ytfryhvwtq ghaatnysyw rntmepyevs wepdfepyiv vsklaprydt rfigfgwnkv
      601 sylthltvlg ykyivlpdtf iihrphapsl digkfrtdsi yrrclkklkd dfveelvtky
      661 gesalsklkk vtkeekilkp vktk
//
LOCUS       XP_034195734             616 aa            linear   INV 03-SEP-2025
DEFINITION  xylosyl- and glucuronyltransferase LARGE1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195734
VERSION     XP_034195734.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339843.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195734.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..616
                     /product="xylosyl- and glucuronyltransferase LARGE1
                     isoform X2"
                     /calculated_mol_wt=71919
     Region          67..344
                     /region_name="GT8_LARGE_C"
                     /note="LARGE catalytic domain has closest homology to GT8
                     glycosyltransferase involved in lipooligosaccharide
                     synthesis; cd06431"
                     /db_xref="CDD:133053"
     Site            order(72..74,77..78,153,167,169..171,199,223..225,
                     261..262,287..288,307,309..310,313)
                     /site_type="active"
                     /note="putative ligand binding site [active]"
                     /db_xref="CDD:133053"
     Site            order(169,171,307)
                     /site_type="active"
                     /note="metal binding site [active]"
                     /db_xref="CDD:133053"
     Region          390..612
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..616
                     /gene="LOC117611705"
                     /coded_by="XM_034339843.2:470..2320"
                     /db_xref="GeneID:117611705"
ORIGIN      
        1 marswlgclf gvlvpsaify lylllnvpsa didsfkivgk eslhverpif dklhsetnrn
       61 eakcetihia mvcagynstf alvtvvksvl fyrtkplhfh llvdeiakrt lttvfrtwdl
      121 phvnltyyka erwvpkvswi pnkhysgvyg llklilpdam redkvlvfdt dvtvvndvnl
      181 lwqmfekftn dqalgltenq shwyikalsy gqrpwpalgr gyntgvmlmh lqqlrnrkft
      241 slwetvtkrv layipetsla dqdimnavik dhpsivykie ctwniqlsdh tisdqcyrdt
      301 srinivhwns prkqdvynkh inefrklhki fldmdgnllr rrlfgcdkhe avseynessp
      361 creftrgatt lyrthpflle yeynvyapad valitqcsve riplledisk hwpgtisval
      421 yltdaelqnf lefvrgsvel rkrkniayhv vykdgelypi nylrniamsy vsspfifqld
      481 vdflpqyglh ealisyivkl nisesdkval ivpafetery rftfpankde llkflkrgvl
      541 ytfryhvwtq ghaatnysyw rntmepyevs wepdfepyiv vsklaprydt rfigfgwnkv
      601 sylthltvlg yntqal
//
LOCUS       XP_034195735             614 aa            linear   INV 03-SEP-2025
DEFINITION  xylosyl- and glucuronyltransferase LARGE1 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195735
VERSION     XP_034195735.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339844.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195735.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..614
                     /product="xylosyl- and glucuronyltransferase LARGE1
                     isoform X3"
                     /calculated_mol_wt=71780
     Region          67..344
                     /region_name="GT8_LARGE_C"
                     /note="LARGE catalytic domain has closest homology to GT8
                     glycosyltransferase involved in lipooligosaccharide
                     synthesis; cd06431"
                     /db_xref="CDD:133053"
     Site            order(72..74,77..78,153,167,169..171,199,223..225,
                     261..262,287..288,307,309..310,313)
                     /site_type="active"
                     /note="putative ligand binding site [active]"
                     /db_xref="CDD:133053"
     Site            order(169,171,307)
                     /site_type="active"
                     /note="metal binding site [active]"
                     /db_xref="CDD:133053"
     Region          390..613
                     /region_name="Glyco_transf_49"
                     /note="Glycosyl-transferase for dystroglycan; pfam13896"
                     /db_xref="CDD:464027"
     CDS             1..614
                     /gene="LOC117611705"
                     /coded_by="XM_034339844.2:470..2314"
                     /db_xref="GeneID:117611705"
ORIGIN      
        1 marswlgclf gvlvpsaify lylllnvpsa didsfkivgk eslhverpif dklhsetnrn
       61 eakcetihia mvcagynstf alvtvvksvl fyrtkplhfh llvdeiakrt lttvfrtwdl
      121 phvnltyyka erwvpkvswi pnkhysgvyg llklilpdam redkvlvfdt dvtvvndvnl
      181 lwqmfekftn dqalgltenq shwyikalsy gqrpwpalgr gyntgvmlmh lqqlrnrkft
      241 slwetvtkrv layipetsla dqdimnavik dhpsivykie ctwniqlsdh tisdqcyrdt
      301 srinivhwns prkqdvynkh inefrklhki fldmdgnllr rrlfgcdkhe avseynessp
      361 creftrgatt lyrthpflle yeynvyapad valitqcsve riplledisk hwpgtisval
      421 yltdaelqnf lefvrgsvel rkrkniayhv vykdgelypi nylrniamsy vsspfifqld
      481 vdflpqyglh ealisyivkl nisesdkval ivpafetery rftfpankde llkflkrgvl
      541 ytfryhvwtq ghaatnysyw rntmepyevs wepdfepyiv vsklaprydt rfigfgwnkv
      601 sylthltvlg ykfi
//
LOCUS       XP_034195736             643 aa            linear   INV 03-SEP-2025
DEFINITION  guanylate cyclase soluble subunit beta-1-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195736
VERSION     XP_034195736.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339845.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..643
                     /product="guanylate cyclase soluble subunit beta-1-like
                     isoform X1"
                     /calculated_mol_wt=72447
     Region          <100..206
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          244..436
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          442..636
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(454,456..461,503,505..507,510,572..574,578..579,
                     582..583,624)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(456,507)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(461,477,480..481,484,488,503..504,560,563,573..576,
                     579,624)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..643
                     /gene="Gycbeta100B"
                     /coded_by="XM_034339845.2:586..2517"
                     /db_xref="GeneID:117611707"
ORIGIN      
        1 mclavvhess cnkrhrtein vqllrtevps rqdsipsesr lleertcplr ltscatktrv
       61 rynehtkygi sfsglcsvdt lnvsmvklrk kvgfnsagpd ipaneilelf grmffefcqd
      121 sgydkilqvl gatprdflqn ldalhdhlgt lypgmrapsf rcterpedga lilhyysdrp
      181 glehivigiv ktvakklhgt diemkilktk necdhvqfli tntsgpgvvs npmiaeletl
      241 svepkvspmt fcrvfpfhlm fnrdltivqt gctitrvipq vssgncklsd illtirphle
      301 ltfenilshi ntvyvlrtkk gvmrvnasee ysylrlkgqm lyipesdfvi flcypsvmnl
      361 ddltrrglyl sdiplhdatr dlvlmseqfe adykltrnle lltdklqqty reldgekqkt
      421 drllysvlpi svanelrhsr pvpakkydcv tllfsgivgf gaycaahtds sgamkivnml
      481 nqlytafdvl tdpkknpnvy kvetvgdkym avsglpepcr charciarla ldmmdlaade
      541 vqidgepvki tigihsgevv tgvighrmpr yclfgntvnl tsrtettgep gkinvseday
      601 rylcmpenqd pqflleyrgp vtmkgksdpm nvwflsrere laa
//
LOCUS       XP_034195739             571 aa            linear   INV 03-SEP-2025
DEFINITION  guanylate cyclase soluble subunit beta-1-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195739
VERSION     XP_034195739.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339848.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..571
                     /product="guanylate cyclase soluble subunit beta-1-like
                     isoform X2"
                     /calculated_mol_wt=64452
     Region          2..134
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          172..364
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          370..564
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(382,384..389,431,433..435,438,500..502,506..507,
                     510..511,552)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(384,435)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(389,405,408..409,412,416,431..432,488,491,501..504,
                     507,552)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..571
                     /gene="Gycbeta100B"
                     /coded_by="XM_034339848.2:279..1994"
                     /db_xref="GeneID:117611707"
ORIGIN      
        1 megqflvrqi yddeitynii saavnrlnip aneilelfgr mffefcqdsg ydkilqvlga
       61 tprdflqnld alhdhlgtly pgmrapsfrc terpedgali lhyysdrpgl ehivigivkt
      121 vakklhgtdi emkilktkne cdhvqflitn tsgpgvvsnp miaeletlsv epkvspmtfc
      181 rvfpfhlmfn rdltivqtgc titrvipqvs sgncklsdil ltirphlelt fenilshint
      241 vyvlrtkkgv mrvnaseeys ylrlkgqmly ipesdfvifl cypsvmnldd ltrrglylsd
      301 iplhdatrdl vlmseqfead ykltrnlell tdklqqtyre ldgekqktdr llysvlpisv
      361 anelrhsrpv pakkydcvtl lfsgivgfga ycaahtdssg amkivnmlnq lytafdvltd
      421 pkknpnvykv etvgdkymav sglpepcrch arciarlald mmdlaadevq idgepvkiti
      481 gihsgevvtg vighrmpryc lfgntvnlts rtettgepgk invsedayry lcmpenqdpq
      541 flleyrgpvt mkgksdpmnv wflsrerela a
//
LOCUS       XP_034195740             571 aa            linear   INV 03-SEP-2025
DEFINITION  guanylate cyclase soluble subunit beta-1-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195740
VERSION     XP_034195740.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339849.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..571
                     /product="guanylate cyclase soluble subunit beta-1-like
                     isoform X2"
                     /calculated_mol_wt=64452
     Region          2..134
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          172..364
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          370..564
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(382,384..389,431,433..435,438,500..502,506..507,
                     510..511,552)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(384,435)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(389,405,408..409,412,416,431..432,488,491,501..504,
                     507,552)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..571
                     /gene="Gycbeta100B"
                     /coded_by="XM_034339849.2:254..1969"
                     /db_xref="GeneID:117611707"
ORIGIN      
        1 megqflvrqi yddeitynii saavnrlnip aneilelfgr mffefcqdsg ydkilqvlga
       61 tprdflqnld alhdhlgtly pgmrapsfrc terpedgali lhyysdrpgl ehivigivkt
      121 vakklhgtdi emkilktkne cdhvqflitn tsgpgvvsnp miaeletlsv epkvspmtfc
      181 rvfpfhlmfn rdltivqtgc titrvipqvs sgncklsdil ltirphlelt fenilshint
      241 vyvlrtkkgv mrvnaseeys ylrlkgqmly ipesdfvifl cypsvmnldd ltrrglylsd
      301 iplhdatrdl vlmseqfead ykltrnlell tdklqqtyre ldgekqktdr llysvlpisv
      361 anelrhsrpv pakkydcvtl lfsgivgfga ycaahtdssg amkivnmlnq lytafdvltd
      421 pkknpnvykv etvgdkymav sglpepcrch arciarlald mmdlaadevq idgepvkiti
      481 gihsgevvtg vighrmpryc lfgntvnlts rtettgepgk invsedayry lcmpenqdpq
      541 flleyrgpvt mkgksdpmnv wflsrerela a
//
LOCUS       XP_034195737             603 aa            linear   INV 03-SEP-2025
DEFINITION  guanylate cyclase soluble subunit beta-1-like isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034195737
VERSION     XP_034195737.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339846.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..603
                     /product="guanylate cyclase soluble subunit beta-1-like
                     isoform X3"
                     /calculated_mol_wt=68132
     Region          2..166
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          204..396
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          402..596
                     /region_name="Guanylate_cyc"
                     /note="Adenylate and Guanylate cyclase catalytic domain;
                     pfam00211"
                     /db_xref="CDD:425528"
     Site            order(414,416..421,463,465..467,470,532..534,538..539,
                     542..543,584)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(416,467)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(421,437,440..441,444,448,463..464,520,523,533..536,
                     539,584)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..603
                     /gene="Gycbeta100B"
                     /coded_by="XM_034339846.2:74..1885"
                     /db_xref="GeneID:117611707"
ORIGIN      
        1 mygfvnyale llvmktfdse tweaikkdaa vnmegqflvr qiyddeityn iisaavnrln
       61 ipaneilelf grmffefcqd sgydkilqvl gatprdflqn ldalhdhlgt lypgmrapsf
      121 rcterpedga lilhyysdrp glehivigiv ktvakklhgt diemkilktk necdhvqfli
      181 tntsgpgvvs npmiaeletl svepkvspmt fcrvfpfhlm fnrdltivqt gctitrvipq
      241 vssgncklsd illtirphle ltfenilshi ntvyvlrtkk gvmrvnasee ysylrlkgqm
      301 lyipesdfvi flcypsvmnl ddltrrglyl sdiplhdatr dlvlmseqfe adykltrnle
      361 lltdklqqty reldgekqkt drllysvlpi svanelrhsr pvpakkydcv tllfsgivgf
      421 gaycaahtds sgamkivnml nqlytafdvl tdpkknpnvy kvetvgdkym avsglpepcr
      481 charciarla ldmmdlaade vqidgepvki tigihsgevv tgvighrmpr yclfgntvnl
      541 tsrtettgep gkinvseday rylcmpenqd pqflleyrgp vtmkgksdpm nvwflsrere
      601 laa
//
LOCUS       XP_034195745             484 aa            linear   INV 03-SEP-2025
DEFINITION  synaptotagmin-10 [Osmia lignaria lignaria].
ACCESSION   XP_034195745
VERSION     XP_034195745.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339854.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="synaptotagmin-10"
                     /calculated_mol_wt=54995
     Region          193..315
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          325..458
                     /region_name="C2B_Synaptotagmin-3-5-6-9-10"
                     /note="C2 domain second repeat present in Synaptotagmins
                     3, 5, 6, 9, and 10; cd08403"
                     /db_xref="CDD:176048"
     Site            order(354,360,384,414,416,422)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176048"
     CDS             1..484
                     /gene="LOC117611709"
                     /coded_by="XM_034339854.2:1831..3285"
                     /db_xref="GeneID:117611709"
ORIGIN      
        1 mgvvktases ilaetvipse eanysvqgha eewtetfpyq llvgtgvalm allllaavsf
       61 qcsstwrwlm rmrrnvkeed aesdlsqqna irishslpdl qsepitheyv qeqkdskkvl
      121 rqttlpivpm rhqsfqrqls hrldlpnikf sicslenrsd sslglikpel ykkellkqdd
      181 adssstaeme fagslrfglf ydkeiesliv kvleardlpe kdvtgssdpy vkvyllpdrk
      241 kkhqtkvhrk nlnpvfnetf ifsvsyeelg erylqfsvyd fdrfsrndli gqvvvkglle
      301 cadleheley tmdimrtmqe kvdlgklmls lcylptagrl tvtvikarnl kamditglsd
      361 pyvkiyllcq gkrikkkktt vkkntlcpvy neilvfdvpa eniedvslii kvidydrigs
      421 nelmgctaig ssfigigrdh wlemldnprk pvtqwyplme tvaghipavn seplpvslsc
      481 lnsr
//
LOCUS       XP_034195746             442 aa            linear   INV 03-SEP-2025
DEFINITION  growth arrest-specific protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034195746
VERSION     XP_034195746.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339855.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..442
                     /product="growth arrest-specific protein 2"
                     /calculated_mol_wt=49477
     Region          56..185
                     /region_name="CH_GAS2-like"
                     /note="calponin homology (CH) domain found in the growth
                     arrest-specific protein 2 family; cd21204"
                     /db_xref="CDD:409053"
     Site            order(61,65,134,136..137,140..141,143,153..161,167,
                     169..170,172..173,176..177,180)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409053"
     Region          329..404
                     /region_name="GAS2"
                     /note="Growth-Arrest-Specific Protein 2 Domain; pfam02187"
                     /db_xref="CDD:460480"
     CDS             1..442
                     /gene="LOC117611710"
                     /coded_by="XM_034339855.2:822..2150"
                     /db_xref="GeneID:117611710"
ORIGIN      
        1 msytnrfpss qhtryrsswg ptsvtvfnra dvprpspeee efaefyherl hsaqsrqllp
       61 lqedladwin ktlnadivtg dnffealdng vvvcrlarvi qekartaida grakgplpli
      121 rgrcwenaar rsffsrdnme nfiqfcrrlg vhenllfesd dlvlhgqprn vvlcllevar
      181 lasrysvepp glvqlereia aeqerdlhcl sdsgishssl vswqfqspsp tatprpqpek
      241 ikhsssasev antgtrwldp ttgvthepem rrsvsdngpt gsdgvpsdtt eddwsrgsae
      301 dpdevpspss splpspvepp ehtgpiteld rkvrrateav qrlcqcpsek csklkvrkvg
      361 egryhiagrn vfirllkgrh mmvrvgggwd tlehflarhd pcqvrtlnrd stppsphgnk
      421 hanflpirak yrspppesvs ar
//
LOCUS       XP_034195748             442 aa            linear   INV 03-SEP-2025
DEFINITION  growth arrest-specific protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_034195748
VERSION     XP_034195748.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339857.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..442
                     /product="growth arrest-specific protein 2"
                     /calculated_mol_wt=49477
     Region          56..185
                     /region_name="CH_GAS2-like"
                     /note="calponin homology (CH) domain found in the growth
                     arrest-specific protein 2 family; cd21204"
                     /db_xref="CDD:409053"
     Site            order(61,65,134,136..137,140..141,143,153..161,167,
                     169..170,172..173,176..177,180)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409053"
     Region          329..404
                     /region_name="GAS2"
                     /note="Growth-Arrest-Specific Protein 2 Domain; pfam02187"
                     /db_xref="CDD:460480"
     CDS             1..442
                     /gene="LOC117611710"
                     /coded_by="XM_034339857.2:580..1908"
                     /db_xref="GeneID:117611710"
ORIGIN      
        1 msytnrfpss qhtryrsswg ptsvtvfnra dvprpspeee efaefyherl hsaqsrqllp
       61 lqedladwin ktlnadivtg dnffealdng vvvcrlarvi qekartaida grakgplpli
      121 rgrcwenaar rsffsrdnme nfiqfcrrlg vhenllfesd dlvlhgqprn vvlcllevar
      181 lasrysvepp glvqlereia aeqerdlhcl sdsgishssl vswqfqspsp tatprpqpek
      241 ikhsssasev antgtrwldp ttgvthepem rrsvsdngpt gsdgvpsdtt eddwsrgsae
      301 dpdevpspss splpspvepp ehtgpiteld rkvrrateav qrlcqcpsek csklkvrkvg
      361 egryhiagrn vfirllkgrh mmvrvgggwd tlehflarhd pcqvrtlnrd stppsphgnk
      421 hanflpirak yrspppesvs ar
//
LOCUS       XP_076546842             442 aa            linear   INV 03-SEP-2025
DEFINITION  growth arrest-specific protein 2 [Osmia lignaria lignaria].
ACCESSION   XP_076546842
VERSION     XP_076546842.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690727.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..442
                     /product="growth arrest-specific protein 2"
                     /calculated_mol_wt=49477
     Region          56..185
                     /region_name="CH_GAS2-like"
                     /note="calponin homology (CH) domain found in the growth
                     arrest-specific protein 2 family; cd21204"
                     /db_xref="CDD:409053"
     Site            order(61,65,134,136..137,140..141,143,153..161,167,
                     169..170,172..173,176..177,180)
                     /site_type="other"
                     /note="putative actin binding site [polypeptide binding]"
                     /db_xref="CDD:409053"
     Region          329..404
                     /region_name="GAS2"
                     /note="Growth-Arrest-Specific Protein 2 Domain; pfam02187"
                     /db_xref="CDD:460480"
     CDS             1..442
                     /gene="LOC117611710"
                     /coded_by="XM_076690727.1:232..1560"
                     /db_xref="GeneID:117611710"
ORIGIN      
        1 msytnrfpss qhtryrsswg ptsvtvfnra dvprpspeee efaefyherl hsaqsrqllp
       61 lqedladwin ktlnadivtg dnffealdng vvvcrlarvi qekartaida grakgplpli
      121 rgrcwenaar rsffsrdnme nfiqfcrrlg vhenllfesd dlvlhgqprn vvlcllevar
      181 lasrysvepp glvqlereia aeqerdlhcl sdsgishssl vswqfqspsp tatprpqpek
      241 ikhsssasev antgtrwldp ttgvthepem rrsvsdngpt gsdgvpsdtt eddwsrgsae
      301 dpdevpspss splpspvepp ehtgpiteld rkvrrateav qrlcqcpsek csklkvrkvg
      361 egryhiagrn vfirllkgrh mmvrvgggwd tlehflarhd pcqvrtlnrd stppsphgnk
      421 hanflpirak yrspppesvs ar
//
LOCUS       XP_034195751             421 aa            linear   INV 03-SEP-2025
DEFINITION  septin 2 [Osmia lignaria lignaria].
ACCESSION   XP_034195751
VERSION     XP_034195751.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339860.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..421
                     /product="septin 2"
                     /calculated_mol_wt=48565
     Region          40..308
                     /region_name="CDC_Septin"
                     /note="CDC/Septin GTPase family; cd01850"
                     /db_xref="CDD:206649"
     Site            50..57
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206649"
     Site            order(52..58,102,105,185..186,188,240..241)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206649"
     Site            77..85
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206649"
     Site            79
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206649"
     Site            102..105
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206649"
     Site            order(104..139,141..151)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206649"
     Site            185..188
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206649"
     Site            240..242
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206649"
     CDS             1..421
                     /gene="Septin2"
                     /coded_by="XM_034339860.2:58..1323"
                     /db_xref="GeneID:117611712"
ORIGIN      
        1 masveverak lessirnlkl sghvgfdslp dqlvnksvqn gfvfnilcig etglgkstlm
       61 dslfntsfes tqsphnlpsv klkahtyelq esnvrlklti vdtvgygdqi nkedsfkavv
      121 dyidaqfeay lqeelkikrc laayhdsrih vclyficptg hglksidlvc mkkldtkvni
      181 ipiiakadti sktelqkfks kiiselqnng vhiyqfptdd esvaevntsm nahvpfavvg
      241 stdfvkvgnk mmrsrqypwg tvqveneshc dfvklremli rtnmedmrek thcrhyelyr
      301 kkrleqmgfs dvdsenkpvs fqqtceakrs ihlqelqqke demrqmfvvr vkekeaelke
      361 aekelhnkfd klkkdhteek kkleesrkkl eddilefnrr ktqfaqqpqh htltlgkskk
      421 k
//
LOCUS       XP_034195754             344 aa            linear   INV 03-SEP-2025
DEFINITION  SOCS domain-containing protein stops [Osmia lignaria lignaria].
ACCESSION   XP_034195754
VERSION     XP_034195754.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339863.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..344
                     /product="SOCS domain-containing protein stops"
                     /calculated_mol_wt=39667
     Region          300..339
                     /region_name="SOCS"
                     /note="SOCS (suppressors of cytokine signaling) box. The
                     SOCS box is found in the C-terminal region of CIS/SOCS
                     family proteins (in combination with a SH2 domain), ASBs
                     (ankyrin repeat-containing proteins with a SOCS box), SSBs
                     (SPRY domain-containing proteins...; cd03587"
                     /db_xref="CDD:239641"
     Site            order(300..305,311,323,329)
                     /site_type="active"
                     /note="elongin B/C interaction [active]"
                     /db_xref="CDD:239641"
     CDS             1..344
                     /gene="stops"
                     /coded_by="XM_034339863.2:107..1141"
                     /db_xref="GeneID:117611715"
ORIGIN      
        1 mevlidcyfd klfaemersc lasrykrrem vgyfsdvins caeaenldkq dvcerivmsa
       61 lryhniamme ngyvcmlgkf hnvlyvaakl cfdwnlnnne ivarllndif ycektferif
      121 vgaifgirvt hflsgwksdf edreeniral vyflnhaalg rleyrcessp mkrrfidvpm
      181 esygqalplr vaiqhgapdi llimlrygas vesdnlapsp lemlltklne yeaqpgqdet
      241 aypeqlllcl rlvlrtvtta fvktpshiad qsgvlsisly eqypalveqk lvppersgls
      301 ppelrhlcrc riretlfenw slphgihqlq lpeslrnyld llyd
//
LOCUS       XP_034196243            1516 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611903 [Osmia lignaria lignaria].
ACCESSION   XP_034196243
VERSION     XP_034196243.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340352.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196243.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1516
                     /product="uncharacterized protein LOC117611903"
                     /calculated_mol_wt=165597
     CDS             1..1516
                     /gene="LOC117611903"
                     /coded_by="XM_034340352.2:358..4908"
                     /db_xref="GeneID:117611903"
ORIGIN      
        1 masyrsygdt gayrsrvgia agtfgraass tgsslpscrn syssigsgss rtlakdrvsg
       61 fgnaflkkdk neekpsfkys tgkdadksrl hpgrsssedv raksplsssr ssllekysyp
      121 aaktsdrpas vldrynrats reksrepegv srygsntypg sglarsygne sriekkerad
      181 hppvsyrglr pnsgrssrep spevsvgksn tfrmyprass ygrsgasssa sessstpiss
      241 rygsssatrf lssrtgndrv paaisysgsd rfrtsgskpv pgsskkedvt kspsekdpsq
      301 epfvtsakae efevdkrlqe setvtitmit rgtspsppas ssfvrnrrad vgiasqkeva
      361 rvrkkidskd qevqcekted nsrflryggg griagtpwtt yidkfsssnt agspgygrgf
      421 nggaggnsrm nsfafsrsnd rndpsmkvsp nssqeifanr nqneqnnkvf gglgfprssa
      481 dpkenprkdq segklqnasc svgkvegkvg rlaspqqsgi vgksqeskse etqssresst
      541 sksesynqrr psiprlgrgt pkndvkilrs ssnsstksdg lrerrgstpk sdhsslskld
      601 essriveghc keqgseiasp ksdnasggke sfhgssqsrs ttpqsrsgss knlprqmtri
      661 dssdssavnv pigrsksssa ssttsqgaki katpscsspg kssvgssass vnlrqggspd
      721 argkppvpks daasssssks ivalkyvnkd frkstlnmen gdpsrskysr kkksnqrsvs
      781 vssqdsqsep nsepirqaat sdssgsvkss rsrlkmplst sktitksdst gnsfrpeksr
      841 stskspsvrr eengtsssss nesnssnsss seeddeeqqi rsssrrrrkr tskspfcekk
      901 gkmsagssrt svlassadem sltmdkpprp pssprsrsdr sgkteeaksf lmralapvtn
      961 lfknkhqdss esgksggwld sneentdvkk sehngtslsk slsksfsftn sekteerkqq
     1021 sngrirrnys geqpwwmnpn cdnvpegvet gsvcnddisq ettvsgalpd dgkfkykvwr
     1081 qeseerawwf dandsvasee akkqssttts rqesvekgrw ldsndsgpse etkkqsfsta
     1141 smhdtgerar wldgndshps eeskksscst gwrqesgdrg cwldandgvp sddskkpfpa
     1201 aspvqkress rsstrssdkr srikhqqsge rawwmsndpd nvpegievip ittdtaenin
     1261 dypdevddsq sygrmfnkir hiesgekpww mdssskvpdg vvkipvetsn stsdssesfe
     1321 ridigvnpep eiytkrslsr fpiefppppp deplgdrasp egvenppdpp dnyggrdspy
     1381 dnvqptprrs sprkrpstlp lfigehtnid dllgdtsnlc pspvlsrirk repieedtde
     1441 esseceeida tqviihdstp ktpviqrrhr dnerpqpdgy ipirilkver smeereklgr
     1501 rnirvffsrh eppstv
//
LOCUS       XP_034196244            1252 aa            linear   INV 03-SEP-2025
DEFINITION  formin homology 2 domain containing isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196244
VERSION     XP_034196244.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340353.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1252
                     /product="formin homology 2 domain containing isoform X1"
                     /calculated_mol_wt=141620
     Region          4..122
                     /region_name="Formin_GBD_N"
                     /note="Formin N-terminal GTPase-binding domain; pfam18382"
                     /db_xref="CDD:465735"
     Region          738..1102
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     CDS             1..1252
                     /gene="Fhos"
                     /coded_by="XM_034340353.2:658..4416"
                     /db_xref="GeneID:117611904"
ORIGIN      
        1 msltcrvqyl ndvdpfayas sypepprppv htfsatlpli nqlaavhrll raphrlddta
       61 lqlykdgdyg ayldleasis eqqeefegfq tnrknsivlr tqlsvrvhti iekllnsegr
      121 elrralfslk qifqedkdlv hefvqndgla clikvgsead qnyqnyilra lgqvmlyvdg
      181 mngvmehgqt vqwlytlias rfrlvvktal klllvfveyv etnslllvra vrsvdtsrgm
      241 ipwtnvmkll kdydaadtel liyattlvnk clngipdqdt yydqvdclee qgiegiiqry
      301 mskqgtdldl lrqfqiyeav lhhedgndrg spirqlddni rktlrnrksl vdsherrksr
      361 rhstgtspls mslnarlspr lnhslglssl nstlnsslpd dddessssqs sqghlgevql
      421 ngsykenkvd vgvtpalrrr reraerqrsf ireqqeatan mrasvvpsdd qespyptngl
      481 rytngtsyer nadspsnkls rtnsrkdltp lmnaanklds eekkpwygks pdegvecdeg
      541 nhtdddedrr vvlqlkregt vkdltqklaa qnlipsspve ekvsrigdms gliskakegl
      601 akskskadvl ksptgdnlpk lqetkksene lhweelvkkl krplalcdld ftdlnsddev
      661 dvlgpvnvtn gippppppmv pspggmrapp ppplgarlpp pipqappplf siglkstrps
      721 tatdssnspk sppptftkks kktvklfwke vrddpiilsr ldknkmiwde lspvpvdtqk
      781 lehlfesrak dlitkkqqem nknkeiivld hkrsnainig mtklppprsi ktailkmdat
      841 imnregiekl ltmlpteeer sriqeaqaan pdlplgsaeq flltlasise lparlklwaf
      901 kldfenseke iadplmdlkq gmetlrvnkt frgilstlls igiflngnev kgfqleylak
      961 vpevkdtvhk hsllhhlchm vmekfpdstd lyseigavtr askidfdela snisklesec
     1021 kaswdhlkli akhdgstmmk vkmsdfladc aeriivlgiv hrriinrfhk fvlwlgiplh
     1081 rvqdtkpnef crivsefale yrttrerviq qlekkanhre rnktrgkmit evgkfrtked
     1141 radaelrqll gsdisdvesi hgtlpwrrqr kdgrtalgpl lrdentngnl tdgddelles
     1201 lvktatktpa trttprerkr trhadrkslr rtlknglsee ekkhiaayik gy
//
LOCUS       XP_034196245            1166 aa            linear   INV 03-SEP-2025
DEFINITION  formin homology 2 domain containing isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196245
VERSION     XP_034196245.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340354.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1166
                     /product="formin homology 2 domain containing isoform X2"
                     /calculated_mol_wt=131930
     Region          <6..35
                     /region_name="Formin_GBD_N"
                     /note="Formin N-terminal GTPase-binding domain; pfam18382"
                     /db_xref="CDD:465735"
     Region          651..1016
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     CDS             1..1166
                     /gene="Fhos"
                     /coded_by="XM_034340354.2:229..3729"
                     /db_xref="GeneID:117611904"
ORIGIN      
        1 mppycrknsi vlrtqlsvrv htiiekllns egrelrralf slkqifqedk dlvhefvqnd
       61 glaclikvgs eadqnyqnyi lralgqvmly vdgmngvmeh gqtvqwlytl iasrfrlvvk
      121 talklllvfv eyvetnslll vravrsvdts rgmipwtnvm kllkdydaad telliyattl
      181 vnkclngipd qdtyydqvdc leeqgiegii qrymskqgtd ldllrqfqiy eavlhhedgn
      241 drgspirqld dnirktlrnr kslvdsherr ksrrhstgts plsmslnarl sprlnhslgl
      301 sslnstlnss lpddddesss sqssqghlge vqlngsyken kvdvgvtpal rrrreraerq
      361 rsfireqqea tanmrasvvp sddqespypt nglrytngts yernadspsn klsrtnsrkd
      421 ltplmnaank ldseekkpwy gkspdegvec degnhtddde drrvvlqlkr egtvkdltqk
      481 laaqnlipss pveekvsrig dmsgliskak eglakskska dvlksptgdn lpklqetkks
      541 enelhweelv kklkrplalc dldftdlnsd devdvlgpvn vtngippppp pmvpspggmr
      601 appppplgar lpppipqapp plfsiglkst rpstatdssn spksppptft kkskktvklf
      661 wkevrddpii lsrldknkmi wdelspvpvd tqklehlfes rakdlitkek qqemnknkei
      721 ivldhkrsna inigmtklpp prsiktailk mdatimnreg ieklltmlpt eeersriqea
      781 qaanpdlplg saeqflltla siselparlk lwafkldfen sekeiadplm dlkqgmetlr
      841 vnktfrgils tllsigifln gnevkgfqle ylakvpevkd tvhkhsllhh lchmvmekfp
      901 dstdlyseig avtraskidf delasniskl eseckaswdh lkliakhdgs tmmkvkmsdf
      961 ladcaeriiv lgivhrriin rfhkfvlwlg iplhrvqdtk pnefcrivse faleyrttre
     1021 rviqqlekka nhrernktrg kmitevgkfr tkedradael rqllgsdisd vesihgtlpw
     1081 rrqrkdgrta lgpllrdent ngnltdgdde lleslvktat ktpatrttpr erkrtrhadr
     1141 kslrrtlkng lseeekkhia ayikgy
//
LOCUS       XP_034196246            1243 aa            linear   INV 03-SEP-2025
DEFINITION  formin homology 2 domain containing isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196246
VERSION     XP_034196246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1243
                     /product="formin homology 2 domain containing isoform X3"
                     /calculated_mol_wt=140578
     Region          4..122
                     /region_name="Formin_GBD_N"
                     /note="Formin N-terminal GTPase-binding domain; pfam18382"
                     /db_xref="CDD:465735"
     Region          738..1102
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     CDS             1..1243
                     /gene="Fhos"
                     /coded_by="XM_034340355.2:657..4388"
                     /db_xref="GeneID:117611904"
ORIGIN      
        1 msltcrvqyl ndvdpfayas sypepprppv htfsatlpli nqlaavhrll raphrlddta
       61 lqlykdgdyg ayldleasis eqqeefegfq tnrknsivlr tqlsvrvhti iekllnsegr
      121 elrralfslk qifqedkdlv hefvqndgla clikvgsead qnyqnyilra lgqvmlyvdg
      181 mngvmehgqt vqwlytlias rfrlvvktal klllvfveyv etnslllvra vrsvdtsrgm
      241 ipwtnvmkll kdydaadtel liyattlvnk clngipdqdt yydqvdclee qgiegiiqry
      301 mskqgtdldl lrqfqiyeav lhhedgndrg spirqlddni rktlrnrksl vdsherrksr
      361 rhstgtspls mslnarlspr lnhslglssl nstlnsslpd dddessssqs sqghlgevql
      421 ngsykenkvd vgvtpalrrr reraerqrsf ireqqeatan mrasvvpsdd qespyptngl
      481 rytngtsyer nadspsnkls rtnsrkdltp lmnaanklds eekkpwygks pdegvecdeg
      541 nhtdddedrr vvlqlkregt vkdltqklaa qnlipsspve ekvsrigdms gliskakegl
      601 akskskadvl ksptgdnlpk lqetkksene lhweelvkkl krplalcdld ftdlnsddev
      661 dvlgpvnvtn gippppppmv pspggmrapp ppplgarlpp pipqappplf siglkstrps
      721 tatdssnspk sppptftkks kktvklfwke vrddpiilsr ldknkmiwde lspvpvdtqk
      781 lehlfesrak dlitkkqqem nknkeiivld hkrsnainig mtklppprsi ktailkmdat
      841 imnregiekl ltmlpteeer sriqeaqaan pdlplgsaeq flltlasise lparlklwaf
      901 kldfenseke iadplmdlkq gmetlrvnkt frgilstlls igiflngnev kgfqleylak
      961 vpevkdtvhk hsllhhlchm vmekfpdstd lyseigavtr askidfdela snisklesec
     1021 kaswdhlkli akhdgstmmk vkmsdfladc aeriivlgiv hrriinrfhk fvlwlgiplh
     1081 rvqdtkpnef crivsefale yrttrerviq qlekkanhre rnktrgkmit evgkfrtked
     1141 radaelrqll gsdisdvesi hgtlpwrrqr kdgrtalgpl lrdentngnl tdgddelles
     1201 lvktatktpa trttprerkr trhadlkrsr trennttpeg yqp
//
LOCUS       XP_034196247            1156 aa            linear   INV 03-SEP-2025
DEFINITION  formin homology 2 domain containing isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196247
VERSION     XP_034196247.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340356.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1156
                     /product="formin homology 2 domain containing isoform X4"
                     /calculated_mol_wt=130759
     Region          <6..35
                     /region_name="Formin_GBD_N"
                     /note="Formin N-terminal GTPase-binding domain; pfam18382"
                     /db_xref="CDD:465735"
     Region          651..1015
                     /region_name="FH2"
                     /note="Formin Homology 2 Domain; pfam02181"
                     /db_xref="CDD:396655"
     CDS             1..1156
                     /gene="Fhos"
                     /coded_by="XM_034340356.2:4..3474"
                     /db_xref="GeneID:117611904"
ORIGIN      
        1 mppycrknsi vlrtqlsvrv htiiekllns egrelrralf slkqifqedk dlvhefvqnd
       61 glaclikvgs eadqnyqnyi lralgqvmly vdgmngvmeh gqtvqwlytl iasrfrlvvk
      121 talklllvfv eyvetnslll vravrsvdts rgmipwtnvm kllkdydaad telliyattl
      181 vnkclngipd qdtyydqvdc leeqgiegii qrymskqgtd ldllrqfqiy eavlhhedgn
      241 drgspirqld dnirktlrnr kslvdsherr ksrrhstgts plsmslnarl sprlnhslgl
      301 sslnstlnss lpddddesss sqssqghlge vqlngsyken kvdvgvtpal rrrreraerq
      361 rsfireqqea tanmrasvvp sddqespypt nglrytngts yernadspsn klsrtnsrkd
      421 ltplmnaank ldseekkpwy gkspdegvec degnhtddde drrvvlqlkr egtvkdltqk
      481 laaqnlipss pveekvsrig dmsgliskak eglakskska dvlksptgdn lpklqetkks
      541 enelhweelv kklkrplalc dldftdlnsd devdvlgpvn vtngippppp pmvpspggmr
      601 appppplgar lpppipqapp plfsiglkst rpstatdssn spksppptft kkskktvklf
      661 wkevrddpii lsrldknkmi wdelspvpvd tqklehlfes rakdlitkkq qemnknkeii
      721 vldhkrsnai nigmtklppp rsiktailkm datimnregi eklltmlpte eersriqeaq
      781 aanpdlplgs aeqflltlas iselparlkl wafkldfens ekeiadplmd lkqgmetlrv
      841 nktfrgilst llsigiflng nevkgfqley lakvpevkdt vhkhsllhhl chmvmekfpd
      901 stdlyseiga vtraskidfd elasniskle seckaswdhl kliakhdgst mmkvkmsdfl
      961 adcaeriivl givhrriinr fhkfvlwlgi plhrvqdtkp nefcrivsef aleyrttrer
     1021 viqqlekkan hrernktrgk mitevgkfrt kedradaelr qllgsdisdv esihgtlpwr
     1081 rqrkdgrtal gpllrdentn gnltdgddel leslvktatk tpatrttpre rkrtrhadlk
     1141 rsrtrenntt pegyqp
//
LOCUS       XP_034196339            1367 aa            linear   INV 03-SEP-2025
DEFINITION  ER degradation enhancer, mannosidase alpha-like 2 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034196339
VERSION     XP_034196339.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340448.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196339.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1367
                     /product="ER degradation enhancer, mannosidase alpha-like
                     2 isoform X1"
                     /calculated_mol_wt=156203
     Region          47..484
                     /region_name="Glyco_hydro_47"
                     /note="Glycosyl hydrolase family 47; pfam01532"
                     /db_xref="CDD:460241"
     Region          635..760
                     /region_name="PA"
                     /note="Protease-associated (PA) domain. The PA domain is
                     an insert domain in a diverse fraction of proteases. The
                     significance of the PA domain to many of the proteins in
                     which it is inserted is undetermined. It may be a
                     protein-protein interaction domain. At...; cl28883"
                     /db_xref="CDD:333703"
     Site            718..720
                     /site_type="other"
                     /note="PA/protease or protease-like domain interface
                     [polypeptide binding]"
                     /db_xref="CDD:238300"
     Region          <1139..>1362
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..1367
                     /gene="Edem2"
                     /coded_by="XM_034340448.2:740..4843"
                     /db_xref="GeneID:117611937"
ORIGIN      
        1 macdsliwgl lifgcsiqal laasednple ymskeereal keeardmfyh aynaymdnay
       61 padelmplsc kgryrgsepn rgdidstlgn fsltlvdtld tlvvlgdlee fenavklvar
      121 dvsfdtdviv slfetnirml ggllsghila eylqqradim pwyrgellnl akdlgyrflp
      181 afntttgipy grinlkygmk gvpleisret ctacagsmil emaalsrltg esvfeekaqk
      241 amdvlwrmrh rgtdlmgsvl nvhsgdwvrr dsgvgagids yyeyclkayi llgdekylgr
      301 fnkhyqavmk yvsqgpmlld vhmhrpntns knfmdallaf wpglqvlkgd ikpavethem
      361 lyqvmqrhnf ipeafttdfq vhwghhplrp eflestyfly ratgdhyylg vgrkvlkslq
      421 tyarvpcgya avsdvrtnkh ddtmdsfvls etfkylfllf tepgelvldl defifttegh
      481 llpltlasir tnvssqdyer divhvdemdr tcpnslhlfp asvrqplrnm vedicprrai
      541 krrlsasqfq annlehlkll sdmgittltl adgrvqllyt fsnaktpqds eegllfmqem
      601 veiskmqsqq tetkpqavtf ikpgtyprqk vtllagpahf glelqgsnki tekvaftyps
      661 facsdlhdta tiagkiaimy rgncmfieka rriqkagaia givldnvdgs saatspmfam
      721 sgdgkevddv tipvvflfst easellkala atggdltvti gnffakddvq lkaptdlsmf
      781 erwkgslksf ltrqmgtpqt fpssgtnldm vpetkqedak dvvvyhelra eiiedvlgge
      841 vrivspylsv hriksegntm iekqwhqlkt vfvnqmyfig kqkpgldmrs filesyynwi
      901 vksrdenidv lkvplrekvl wflkelaeil pdpsimimdq lmefnkrkll kqylltkidl
      961 mvlnmravnt mlktmrfesl tteqlfemfk lhiaqaenyt flkqlllgif dvedesitnh
     1021 lilldrvnkd vhneyfeiia gaqetiqkti aeknayyevl iknwekdfsn yerfnkfkkp
     1081 kdydsttiee fmtdnsessk tdiklrdssk mpkkdddiki gkfedfeyfk ladiekavkn
     1141 leqntirkyq eqidtsrdta dniskeaids nkvilnvslq nqsktdklkn kikyqiefik
     1201 nkiedftkdi dsltdftkia nslenvqlnn iefqevisnn nnvekqvnep slneekevll
     1261 eqikkestee csnknnpnlk hsvhlnvntn kiasfnlfed fkkktwstgk eenknsidiq
     1321 qerdisnidl nsvkenklqg sqkyaieies kksdvqehes khiddel
//
LOCUS       XP_076546843            1334 aa            linear   INV 03-SEP-2025
DEFINITION  ER degradation enhancer, mannosidase alpha-like 2 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546843
VERSION     XP_076546843.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690728.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1334
                     /product="ER degradation enhancer, mannosidase alpha-like
                     2 isoform X2"
                     /calculated_mol_wt=152558
     Region          47..484
                     /region_name="Glyco_hydro_47"
                     /note="Glycosyl hydrolase family 47; pfam01532"
                     /db_xref="CDD:460241"
     Region          602..727
                     /region_name="PA"
                     /note="Protease-associated (PA) domain. The PA domain is
                     an insert domain in a diverse fraction of proteases. The
                     significance of the PA domain to many of the proteins in
                     which it is inserted is undetermined. It may be a
                     protein-protein interaction domain. At...; cl28883"
                     /db_xref="CDD:333703"
     Site            685..687
                     /site_type="other"
                     /note="PA/protease or protease-like domain interface
                     [polypeptide binding]"
                     /db_xref="CDD:238300"
     Region          <1106..>1329
                     /region_name="Mplasa_alph_rch"
                     /note="helix-rich Mycoplasma protein; TIGR04523"
                     /db_xref="CDD:275316"
     CDS             1..1334
                     /gene="Edem2"
                     /coded_by="XM_076690728.1:742..4746"
                     /db_xref="GeneID:117611937"
ORIGIN      
        1 macdsliwgl lifgcsiqal laasednple ymskeereal keeardmfyh aynaymdnay
       61 padelmplsc kgryrgsepn rgdidstlgn fsltlvdtld tlvvlgdlee fenavklvar
      121 dvsfdtdviv slfetnirml ggllsghila eylqqradim pwyrgellnl akdlgyrflp
      181 afntttgipy grinlkygmk gvpleisret ctacagsmil emaalsrltg esvfeekaqk
      241 amdvlwrmrh rgtdlmgsvl nvhsgdwvrr dsgvgagids yyeyclkayi llgdekylgr
      301 fnkhyqavmk yvsqgpmlld vhmhrpntns knfmdallaf wpglqvlkgd ikpavethem
      361 lyqvmqrhnf ipeafttdfq vhwghhplrp eflestyfly ratgdhyylg vgrkvlkslq
      421 tyarvpcgya avsdvrtnkh ddtmdsfvls etfkylfllf tepgelvldl defifttegh
      481 llpltlasir tnvssqdyer divhvdemdr tcpnslhlfp asvrqplrnm vedicprrai
      541 krrlsasqfq aktpqdseeg llfmqemvei skmqsqqtet kpqavtfikp gtyprqkvtl
      601 lagpahfgle lqgsnkitek vaftypsfac sdlhdtatia gkiaimyrgn cmfiekarri
      661 qkagaiagiv ldnvdgssaa tspmfamsgd gkevddvtip vvflfsteas ellkalaatg
      721 gdltvtignf fakddvqlka ptdlsmferw kgslksfltr qmgtpqtfps sgtnldmvpe
      781 tkqedakdvv vyhelraeii edvlggevri vspylsvhri ksegntmiek qwhqlktvfv
      841 nqmyfigkqk pgldmrsfil esyynwivks rdenidvlkv plrekvlwfl kelaeilpdp
      901 simimdqlme fnkrkllkqy lltkidlmvl nmravntmlk tmrfesltte qlfemfklhi
      961 aqaenytflk qlllgifdve desitnhlil ldrvnkdvhn eyfeiiagaq etiqktiaek
     1021 nayyevlikn wekdfsnyer fnkfkkpkdy dsttieefmt dnsessktdi klrdsskmpk
     1081 kdddikigkf edfeyfklad iekavknleq ntirkyqeqi dtsrdtadni skeaidsnkv
     1141 ilnvslqnqs ktdklknkik yqiefiknki edftkdidsl tdftkiansl envqlnnief
     1201 qevisnnnnv ekqvnepsln eekevlleqi kkesteecsn knnpnlkhsv hlnvntnkia
     1261 sfnlfedfkk ktwstgkeen knsidiqqer disnidlnsv kenklqgsqk yaieieskks
     1321 dvqeheskhi ddel
//
LOCUS       XP_034196340             539 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611938 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196340
VERSION     XP_034196340.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340449.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..539
                     /product="uncharacterized protein LOC117611938 isoform X1"
                     /calculated_mol_wt=60152
     Region          11..41
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(26,37)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          159..193
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          247..283
                     /region_name="CUE"
                     /note="CUE domain found in ubiquitin-binding CUE proteins;
                     cd14279"
                     /db_xref="CDD:270465"
     CDS             1..539
                     /gene="LOC117611938"
                     /coded_by="XM_034340449.2:61..1680"
                     /db_xref="GeneID:117611938"
ORIGIN      
        1 maeesdrsql ppgwecrydi rsgrpyfinh fnrtttwedp rvrywqysqy iqsqnsmals
       61 sattitsqdi pmqtggngsg ghlgytgahr apqiyptpsp yhnpqlaflp pslqelktpl
      121 slksvsvmtn rfgdmtigsp tpirnmetsl tqnsdtelqv akinamfptv sdthirlllk
      181 kyhnraalvv salqveknpl capgpctpvg vhsnytiprw rlpphaihaa ltlspprgvr
      241 ptphspkmkl rylknvfpkv eetmlldile qsdnnvqkas eklidlgyek rnptaprsqa
      301 krkeeeqmrn vqhaptpppr mksledknki ktrlmekyks vpervimlam dsvdydeeka
      361 ihildimvme eatrpvctss qssrsderkd sppvieavkl taspikkivk dadsekskrs
      421 knkieipkvs rgtsttedne ykspyltkpi gpnpdlpkga dndlllpdya pwsgpdpnll
      481 tneifsktla nghdaslvsg srciakgpns elrkgslrgl tqgsiysqrn vsntesrgk
//
LOCUS       XP_034196341             498 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611938 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196341
VERSION     XP_034196341.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340450.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="uncharacterized protein LOC117611938 isoform X2"
                     /calculated_mol_wt=55912
     Region          11..41
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(26,37)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          118..152
                     /region_name="UBA_like_SF"
                     /note="UBA domain-like superfamily; cl21463"
                     /db_xref="CDD:473871"
     Region          206..242
                     /region_name="CUE"
                     /note="CUE domain found in ubiquitin-binding CUE proteins;
                     cd14279"
                     /db_xref="CDD:270465"
     CDS             1..498
                     /gene="LOC117611938"
                     /coded_by="XM_034340450.2:61..1557"
                     /db_xref="GeneID:117611938"
ORIGIN      
        1 maeesdrsql ppgwecrydi rsgrpyfinh fnrtttwedp rvrywqysqy iqsqnsmals
       61 sattitsqdi pmqelktpls lksvsvmtnr fgdmtigspt pirnmetslt qnsdtelqva
      121 kinamfptvs dthirlllkk yhnraalvvs alqveknplc apgpctpvgv hsnytiprwr
      181 lpphaihaal tlspprgvrp tphspkmklr ylknvfpkve etmlldileq sdnnvqkase
      241 klidlgyekr nptaprsqak rkeeeqmrnv qhaptppprm ksledknkik trlmekyksv
      301 pervimlamd svdydeekai hildimvmee atrpvctssq ssrsderkds ppvieavklt
      361 aspikkivkd adsekskrsk nkieipkvsr gtsttedney kspyltkpig pnpdlpkgad
      421 ndlllpdyap wsgpdpnllt neifsktlan ghdaslvsgs rciakgpnse lrkgslrglt
      481 qgsiysqrnv sntesrgk
//
LOCUS       XP_034196342             469 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611938 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196342
VERSION     XP_034196342.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340451.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="uncharacterized protein LOC117611938 isoform X3"
                     /calculated_mol_wt=52495
     Region          11..41
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(26,37)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          182..213
                     /region_name="CUE"
                     /note="CUE domain found in ubiquitin-binding CUE proteins;
                     cd14279"
                     /db_xref="CDD:270465"
     CDS             1..469
                     /gene="LOC117611938"
                     /coded_by="XM_034340451.2:61..1470"
                     /db_xref="GeneID:117611938"
ORIGIN      
        1 maeesdrsql ppgwecrydi rsgrpyfinh fnrtttwedp rvrywqysqy iqsqnsmals
       61 sattitsqdi pmqtggngsg ghlgytgahr apqiyptpsp yhnpqlaflp pslqelktpl
      121 slksvsvmtn rfgdmtigsp tpirnmetsl tqnsdtelqv akinamfptv sdthirlllk
      181 kylknvfpkv eetmlldile qsdnnvqkas eklidlgyek rnptaprsqa krkeeeqmrn
      241 vqhaptpppr mksledknki ktrlmekyks vpervimlam dsvdydeeka ihildimvme
      301 eatrpvctss qssrsderkd sppvieavkl taspikkivk dadsekskrs knkieipkvs
      361 rgtsttedne ykspyltkpi gpnpdlpkga dndlllpdya pwsgpdpnll tneifsktla
      421 nghdaslvsg srciakgpns elrkgslrgl tqgsiysqrn vsntesrgk
//
LOCUS       XP_034196343             243 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial fission regulator 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196343
VERSION     XP_034196343.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340452.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="mitochondrial fission regulator 2 isoform X1"
                     /calculated_mol_wt=26822
     Region          6..187
                     /region_name="Mito_fiss_reg"
                     /note="Mitochondrial fission regulator; pfam05308"
                     /db_xref="CDD:461620"
     CDS             1..243
                     /gene="LOC117611939"
                     /coded_by="XM_034340452.2:224..955"
                     /db_xref="GeneID:117611939"
ORIGIN      
        1 msagpkyygk rrsivrrigs mlplkpppkv yvsvtplhsm tssmineets gdyvdtngfg
       61 pasrlsnirp dllepisfgs eirllaleqe lqdlreqvsa ivksnkesry tstaslangt
      121 ddipelqqpl pelpeppppp plppptphia rraslqdhkt ddrkkvsfis qasmtdvlkd
      181 idnlklrpva rspggrplri krdnspcndl qeilrrryva mnsadsrnss anltlddsft
      241 sdt
//
LOCUS       XP_034196345             242 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial fission regulator 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196345
VERSION     XP_034196345.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340454.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="mitochondrial fission regulator 2 isoform X2"
                     /calculated_mol_wt=26735
     Region          6..186
                     /region_name="Mito_fiss_reg"
                     /note="Mitochondrial fission regulator; pfam05308"
                     /db_xref="CDD:461620"
     CDS             1..242
                     /gene="LOC117611939"
                     /coded_by="XM_034340454.2:229..957"
                     /db_xref="GeneID:117611939"
ORIGIN      
        1 msagpkyygk rrsivrrigs mlplkpppkv yvsvtplhsm tssmineets gdyvdtngfg
       61 parlsnirpd llepisfgse irllaleqel qdlreqvsai vksnkesryt staslangtd
      121 dipelqqplp elpepppppp lppptphiar raslqdhktd drkkvsfisq asmtdvlkdi
      181 dnlklrpvar spggrplrik rdnspcndlq eilrrryvam nsadsrnssa nltlddsfts
      241 dt
//
LOCUS       XP_034196346             237 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial fission regulator 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196346
VERSION     XP_034196346.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340455.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..237
                     /product="mitochondrial fission regulator 2 isoform X3"
                     /calculated_mol_wt=26152
     Region          6..181
                     /region_name="Mito_fiss_reg"
                     /note="Mitochondrial fission regulator; pfam05308"
                     /db_xref="CDD:461620"
     CDS             1..237
                     /gene="LOC117611939"
                     /coded_by="XM_034340455.2:232..945"
                     /db_xref="GeneID:117611939"
ORIGIN      
        1 msagpkyygk rrsivrrigs mlplkpppkv yvsvtplhsm tssmineets gdyvdtngfg
       61 parpdllepi sfgseirlla leqelqdlre qvsaivksnk esrytstasl angtddipel
      121 qqplpelpep ppppplpppt phiarraslq dhktddrkkv sfisqasmtd vlkdidnlkl
      181 rpvarspggr plrikrdnsp cndlqeilrr ryvamnsads rnssanltld dsftsdt
//
LOCUS       XP_034196347             135 aa            linear   INV 03-SEP-2025
DEFINITION  corazonin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034196347
VERSION     XP_034196347.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340456.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..135
                     /product="corazonin isoform X1"
                     /calculated_mol_wt=15291
     Region          43..>57
                     /region_name="Corazonin"
                     /note="Pro-corazonin; pfam17308"
                     /db_xref="CDD:375119"
     CDS             1..135
                     /gene="Crz"
                     /coded_by="XM_034340456.2:313..720"
                     /db_xref="GeneID:117611940"
ORIGIN      
        1 mkttdsrmis hyigqdgtmr kmvasripvf filsltmttv icqtfqyshg wtngkrsass
       61 vleelvnssg knavqldnvl vncelqklrl llqgnidsqf lqvpcefydt akrnypeima
      121 nehfrrqsst ssnny
//
LOCUS       XP_034196348             114 aa            linear   INV 03-SEP-2025
DEFINITION  corazonin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034196348
VERSION     XP_034196348.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340457.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..114
                     /product="corazonin isoform X2"
                     /calculated_mol_wt=12853
     Region          22..>36
                     /region_name="Corazonin"
                     /note="Pro-corazonin; pfam17308"
                     /db_xref="CDD:375119"
     CDS             1..114
                     /gene="Crz"
                     /coded_by="XM_034340457.2:272..616"
                     /db_xref="GeneID:117611940"
ORIGIN      
        1 mvasripvff ilsltmttvi cqtfqyshgw tngkrsassv leelvnssgk navqldnvlv
       61 ncelqklrll lqgnidsqfl qvpcefydta krnypeiman ehfrrqssts snny
//
LOCUS       XP_034196349             124 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA methyltransferase subunit 11-2 [Osmia lignaria lignaria].
ACCESSION   XP_034196349
VERSION     XP_034196349.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340458.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="tRNA methyltransferase subunit 11-2"
                     /calculated_mol_wt=13780
     Region          2..119
                     /region_name="Trm112-like"
                     /note="eukaryotic tRNA methyltransferase 112, a partner
                     protein of both rRNA/tRNA and protein methyltransferases,
                     and similar proteins; cd21089"
                     /db_xref="CDD:411041"
     Site            order(2,5,7..10,12,21,38,41,44,48,50,112,114..118)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:411041"
     CDS             1..124
                     /gene="Trmt112"
                     /coded_by="XM_034340458.2:166..540"
                     /db_xref="GeneID:117611941"
ORIGIN      
        1 mkllthnmlt skclkgvtvg yplgivakdi kvsevdfnse fiariipkld watlwkaaes
       61 ighvgelpqt liqdfetnee flkkvhhvll evevingdll cpesgrkfpi ndgipnmlln
      121 edev
//
LOCUS       XP_034196350             106 aa            linear   INV 03-SEP-2025
DEFINITION  HIG1 domain family member 1A, mitochondrial-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034196350
VERSION     XP_034196350.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340459.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="HIG1 domain family member 1A,
                     mitochondrial-like"
                     /calculated_mol_wt=11687
     Region          39..90
                     /region_name="HIG_1_N"
                     /note="Hypoxia induced protein conserved region;
                     pfam04588"
                     /db_xref="CDD:461361"
     CDS             1..106
                     /gene="LOC117611942"
                     /coded_by="XM_034340459.2:189..509"
                     /db_xref="GeneID:117611942"
ORIGIN      
        1 mdqyphaepl eipeealklt leneepaglg qsiyktmqrs pfliagilgf gavcgigayk
       61 wktrtikpsl ffiqlrvaaq stalgfitlg mvyqmykdya skqkpt
//
LOCUS       XP_076546844             106 aa            linear   INV 03-SEP-2025
DEFINITION  HIG1 domain family member 1A, mitochondrial-like [Osmia lignaria
            lignaria].
ACCESSION   XP_076546844
VERSION     XP_076546844.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690729.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="HIG1 domain family member 1A,
                     mitochondrial-like"
                     /calculated_mol_wt=11687
     Region          39..90
                     /region_name="HIG_1_N"
                     /note="Hypoxia induced protein conserved region;
                     pfam04588"
                     /db_xref="CDD:461361"
     CDS             1..106
                     /gene="LOC117611942"
                     /coded_by="XM_076690729.1:189..509"
                     /db_xref="GeneID:117611942"
ORIGIN      
        1 mdqyphaepl eipeealklt leneepaglg qsiyktmqrs pfliagilgf gavcgigayk
       61 wktrtikpsl ffiqlrvaaq stalgfitlg mvyqmykdya skqkpt
//
LOCUS       XP_034196351             106 aa            linear   INV 03-SEP-2025
DEFINITION  HIG1 domain family member 1A, mitochondrial-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034196351
VERSION     XP_034196351.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340460.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="HIG1 domain family member 1A,
                     mitochondrial-like"
                     /calculated_mol_wt=11687
     Region          39..90
                     /region_name="HIG_1_N"
                     /note="Hypoxia induced protein conserved region;
                     pfam04588"
                     /db_xref="CDD:461361"
     CDS             1..106
                     /gene="LOC117611942"
                     /coded_by="XM_034340460.2:217..537"
                     /db_xref="GeneID:117611942"
ORIGIN      
        1 mdqyphaepl eipeealklt leneepaglg qsiyktmqrs pfliagilgf gavcgigayk
       61 wktrtikpsl ffiqlrvaaq stalgfitlg mvyqmykdya skqkpt
//
LOCUS       XP_034196352             106 aa            linear   INV 03-SEP-2025
DEFINITION  HIG1 domain family member 1A, mitochondrial-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034196352
VERSION     XP_034196352.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340461.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="HIG1 domain family member 1A,
                     mitochondrial-like"
                     /calculated_mol_wt=11687
     Region          39..90
                     /region_name="HIG_1_N"
                     /note="Hypoxia induced protein conserved region;
                     pfam04588"
                     /db_xref="CDD:461361"
     CDS             1..106
                     /gene="LOC117611942"
                     /coded_by="XM_034340461.2:263..583"
                     /db_xref="GeneID:117611942"
ORIGIN      
        1 mdqyphaepl eipeealklt leneepaglg qsiyktmqrs pfliagilgf gavcgigayk
       61 wktrtikpsl ffiqlrvaaq stalgfitlg mvyqmykdya skqkpt
//
LOCUS       XP_034196367            1570 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611952 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196367
VERSION     XP_034196367.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340476.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196367.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1570
                     /product="uncharacterized protein LOC117611952 isoform X1"
                     /calculated_mol_wt=173334
     Region          106..>542
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          901..970
                     /region_name="WWE"
                     /note="WWE domain; pfam02825"
                     /db_xref="CDD:460715"
     Region          1296..1499
                     /region_name="DDHD"
                     /note="DDHD domain; pfam02862"
                     /db_xref="CDD:460725"
     CDS             1..1570
                     /gene="LOC117611952"
                     /coded_by="XM_034340476.2:81..4793"
                     /db_xref="GeneID:117611952"
ORIGIN      
        1 msgasrkvkn plltaagegf pfdnfaetal lvpvtpatpa vlrddaelds fvgqepinqk
       61 tstdaeneft piplnsdenk nsvnqpakqg yftsilsslp nltlssitgd spgsqtnrqq
      121 ehqssnpyvp sheprdslrd tsppiianfh dprrtnfvgs aegstgsvsa ppqptlpptl
      181 psstngpisy rlgnqrrlky apppdltsnt skqystpvvq safvpqsvav ptifnpnqig
      241 spsqtyesvp ttqnyqsvpl tqtyqsvpat qnhqpvpstq sfqstistqs yksvspiqsn
      301 qsipsteiqq pvpsiqdyqs vpliqstqpv lstqshqsvp liqnykpvsl sqgnqpvqlt
      361 qnnqsvpltq syqsitstgs fqpisstqny hsnastqssk svsptsskqp tpspqsyqsv
      421 sltqtyqsvs ssqssqpiss tqshqpvplt qstqfikpsv ttsspsiqhs tpelsesnsq
      481 qesfrasplt tgsidpsrri sqnknpasds vslsekprvs lsssqpifse fpepatpqnt
      541 panvsffgfd gytskrdssr tpnlekeann sstrsdsilk qnvtqstaek kptvtfgpvs
      601 avqvsekleh llaeqkedls tkpdrseaed rtvgspsvvk sdnirensds pkvdtlsdpi
      661 asqeasrsnl shqessgahe kidasqsdgv ssqqyfqtqs fpssadffad qrsnlntfse
      721 alntftppas tffntnvdls tsslrndlqq vplttsfvsh prnpwdsnqg smvetcssst
      781 sspqpplfyn paqfptelqk histhqfygs pvvnqtqgyf dnlpglvntq eipggigsaf
      841 lpsvvmttvp eptgsatlsp vqmsinplag rtgtdgvpts fqnlaagpsd krmqyrsvyh
      901 hwfyrkeveh kvlwlpfsmr dslrleevyn steitpettv atdggrydvd ilrrqrspvy
      961 wsgkstevrr cswfykgpte sryvpydest alkleeeykq acitnnwnrr idlnngeyii
     1021 fhsatvqvhy ltpssqelva swgnstgsed saylqgagsr prvikrgmde fhiedgepek
     1081 vdhllflvhg igsvcdlkfr tveevvdefr sislqlvqsh yrtaseqrvv nrievlpisw
     1141 httlhsedtg idkklqaitl esipklrhft ndtlldilfy tspvycqtim qtvgieinrl
     1201 ytlfqernpd fnggiylggh slgslilfdl lchqtplpkd ksnednrean edqkneetdp
     1261 sqpspnrilk rslskkinyv mgvagtgqpy inypqlnfhp raffalgspv gmfvtvrgid
     1321 mlgedfilpt cpaffnifhp fdpvayrvea linpeankyr pmliphhkgr krmhlelket
     1381 marvgadlkq kvidsvkntw nsvyqlalfh rpdntllere idkvveeqlq qtppvtdqqn
     1441 nddggaelki gklnggrrid yvlqeapfey ineyifalts hvcywesedt mlmilkeiyg
     1501 sagiqtdaql pqqtlsiert spsmsvvsst ssttkceirp svsptgmdpt apiaektvgp
     1561 ppkagfvrks
//
LOCUS       XP_034196368            1561 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611952 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196368
VERSION     XP_034196368.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340477.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196368.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1561
                     /product="uncharacterized protein LOC117611952 isoform X2"
                     /calculated_mol_wt=172383
     Region          106..>542
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          901..970
                     /region_name="WWE"
                     /note="WWE domain; pfam02825"
                     /db_xref="CDD:460715"
     Region          1287..1490
                     /region_name="DDHD"
                     /note="DDHD domain; pfam02862"
                     /db_xref="CDD:460725"
     CDS             1..1561
                     /gene="LOC117611952"
                     /coded_by="XM_034340477.2:82..4767"
                     /db_xref="GeneID:117611952"
ORIGIN      
        1 msgasrkvkn plltaagegf pfdnfaetal lvpvtpatpa vlrddaelds fvgqepinqk
       61 tstdaeneft piplnsdenk nsvnqpakqg yftsilsslp nltlssitgd spgsqtnrqq
      121 ehqssnpyvp sheprdslrd tsppiianfh dprrtnfvgs aegstgsvsa ppqptlpptl
      181 psstngpisy rlgnqrrlky apppdltsnt skqystpvvq safvpqsvav ptifnpnqig
      241 spsqtyesvp ttqnyqsvpl tqtyqsvpat qnhqpvpstq sfqstistqs yksvspiqsn
      301 qsipsteiqq pvpsiqdyqs vpliqstqpv lstqshqsvp liqnykpvsl sqgnqpvqlt
      361 qnnqsvpltq syqsitstgs fqpisstqny hsnastqssk svsptsskqp tpspqsyqsv
      421 sltqtyqsvs ssqssqpiss tqshqpvplt qstqfikpsv ttsspsiqhs tpelsesnsq
      481 qesfrasplt tgsidpsrri sqnknpasds vslsekprvs lsssqpifse fpepatpqnt
      541 panvsffgfd gytskrdssr tpnlekeann sstrsdsilk qnvtqstaek kptvtfgpvs
      601 avqvsekleh llaeqkedls tkpdrseaed rtvgspsvvk sdnirensds pkvdtlsdpi
      661 asqeasrsnl shqessgahe kidasqsdgv ssqqyfqtqs fpssadffad qrsnlntfse
      721 alntftppas tffntnvdls tsslrndlqq vplttsfvsh prnpwdsnqg smvetcssst
      781 sspqpplfyn paqfptelqk histhqfygs pvvnqtqgyf dnlpglvntq eipggigsaf
      841 lpsvvmttvp eptgsatlsp vqmsinplag rtgtdgvpts fqnlaagpsd krmqyrsvyh
      901 hwfyrkeveh kvlwlpfsmr dslrleevyn steitpettv atdggrydvd ilrrqrspvy
      961 wsgkstevrr cswfykgpte sryvpydest alkleeeykq acitnnwnrr idlnngeyii
     1021 fhsatvqvhy ltpssqelva swgnstgags rprvikrgmd efhiedgepe kvdhllflvh
     1081 gigsvcdlkf rtveevvdef rsislqlvqs hyrtaseqrv vnrievlpis whttlhsedt
     1141 gidkklqait lesipklrhf tndtlldilf ytspvycqti mqtvgieinr lytlfqernp
     1201 dfnggiylgg hslgslilfd llchqtplpk dksnednrea nedqkneetd psqpspnril
     1261 krslskkiny vmgvagtgqp yinypqlnfh praffalgsp vgmfvtvrgi dmlgedfilp
     1321 tcpaffnifh pfdpvayrve alinpeanky rpmliphhkg rkrmhlelke tmarvgadlk
     1381 qkvidsvknt wnsvyqlalf hrpdntller eidkvveeql qqtppvtdqq nnddggaelk
     1441 igklnggrri dyvlqeapfe yineyifalt shvcywesed tmlmilkeiy gsagiqtdaq
     1501 lpqqtlsier tspsmsvvss tssttkceir psvsptgmdp tapiaektvg pppkagfvrk
     1561 s
//
LOCUS       XP_034196370            1044 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611953 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196370
VERSION     XP_034196370.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340479.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196370.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1044
                     /product="uncharacterized protein LOC117611953 isoform X1"
                     /calculated_mol_wt=110881
     CDS             1..1044
                     /gene="LOC117611953"
                     /coded_by="XM_034340479.2:2437..5571"
                     /db_xref="GeneID:117611953"
ORIGIN      
        1 mcfaridrqm sivllsilli ghhvqavvnk vqpqtsvkge edkqneikad drisnsygpp
       61 addygppiga nlkgpapvyg ppelvgdqgp tpiypppppe islpvygppl ssygpprnik
      121 pfygppkqsy gpplsplpsk lsfgslklhy gppkqhygpp ysfgsfrppk pqygpplkft
      181 sgvsnqfias sghrptkpvl epsvslplnt yeppqklavq fnrqpndeyg pppppaisav
      241 pesqygppts dlyappppvp ppgvpapptp pdikydgwqp iaglsnqhgq psntygppln
      301 epktvegsss nlqddpnvps dsygvpihnp eaqdlktsvk lganenaglp ppslpeyepf
      361 hnenqnppkk dlpekilsgp vnlqygvpkv eplpliktvg fellptvspl ssdlsglklp
      421 vldsvphfnl gfgdthafqs lgndlslgqf sanglsnsyg pplagvsfgk lnsiesgipl
      481 pppplldtys ipplssfsln epyqsaeagr asshssfglh gsslfkqnih hhrphgafrs
      541 ppplpgslip prnrepikfk epipsglltn lnryvppprh adlakspktf istlsfeqql
      601 pslsapvafq klqnlgsnsp iaapnaqygt plsfsnfntp apvltygapn fgpassfvst
      661 stsfgnnlyd signtittty gtpvvslpls tdgghdcgfq qtgtgtqysf kdtgnylans
      721 gpqlhsfgaa lsgqslssdl gqnfkvgsld lpqsisqlnl esyeqpktnl kdsygnpvgv
      781 qtapeqldni lasdhsqssd vanvisappv tqfqdspfsp ssqdnsirae altaslseqg
      841 fgqaknlgsn evdasqflns qegsealsla kgltssggdg fevqgskgty mlqiqaadgg
      901 lgtensdgsi rhdqvlsngl lqdilaaieq peqgqvqvqg hpevqslqhv ysnlaeprna
      961 dipkghyitv eegsgkkdig elaelasdrs esefnkadas kkeavalffn nqysdsrket
     1021 rsvakrekvg alenakgask ekss
//
LOCUS       XP_034196371            1040 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611953 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196371
VERSION     XP_034196371.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340480.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196371.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1040
                     /product="uncharacterized protein LOC117611953 isoform X2"
                     /calculated_mol_wt=110288
     CDS             1..1040
                     /gene="LOC117611953"
                     /coded_by="XM_034340480.2:1086..4208"
                     /db_xref="GeneID:117611953"
ORIGIN      
        1 mvgikmsivl lsillighhv qavvnkvqpq tsvkgeedkq neikaddris nsygppaddy
       61 gppiganlkg papvygppel vgdqgptpiy pppppeislp vygpplssyg pprnikpfyg
      121 ppkqsygppl splpsklsfg slklhygppk qhygppysfg sfrppkpqyg pplkftsgvs
      181 nqfiassghr ptkpvlepsv slplntyepp qklavqfnrq pndeygpppp paisavpesq
      241 ygpptsdlya ppppvpppgv papptppdik ydgwqpiagl snqhgqpsnt ygpplnepkt
      301 vegsssnlqd dpnvpsdsyg vpihnpeaqd lktsvklgan enaglpppsl peyepfhnen
      361 qnppkkdlpe kilsgpvnlq ygvpkveplp liktvgfell ptvsplssdl sglklpvlds
      421 vphfnlgfgd thafqslgnd lslgqfsang lsnsygppla gvsfgklnsi esgiplpppp
      481 lldtysippl ssfslnepyq saeagrassh ssfglhgssl fkqnihhhrp hgafrspppl
      541 pgslipprnr epikfkepip sglltnlnry vppprhadla kspktfistl sfeqqlpsls
      601 apvafqklqn lgsnspiaap naqygtplsf snfntpapvl tygapnfgpa ssfvststsf
      661 gnnlydsign titttygtpv vslplstdgg hdcgfqqtgt gtqysfkdtg nylansgpql
      721 hsfgaalsgq slssdlgqnf kvgsldlpqs isqlnlesye qpktnlkdsy gnpvgvqtap
      781 eqldnilasd hsqssdvanv isappvtqfq dspfspssqd nsiraealta slseqgfgqa
      841 knlgsnevda sqflnsqegs ealslakglt ssggdgfevq gskgtymlqi qaadgglgte
      901 nsdgsirhdq vlsngllqdi laaieqpeqg qvqvqghpev qslqhvysnl aeprnadipk
      961 ghyitveegs gkkdigelae lasdrsesef nkadaskkea valffnnqys dsrketrsva
     1021 krekvgalen akgaskekss
//
LOCUS       XP_034196372             772 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611954 [Osmia lignaria lignaria].
ACCESSION   XP_034196372
VERSION     XP_034196372.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340481.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196372.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..772
                     /product="uncharacterized protein LOC117611954"
                     /calculated_mol_wt=87971
     CDS             1..772
                     /gene="LOC117611954"
                     /coded_by="XM_034340481.2:526..2844"
                     /db_xref="GeneID:117611954"
ORIGIN      
        1 mysqalffys viyysivrvq ctaiflttpp vnpidrdsrd rwkfleenvp veegsppfdw
       61 kfqrelpyeg vsiggwvnne ffqkfredkg egtvlfsvde csrycikngs gkddedfqyi
      121 nlfwtvnitd itgdllsndl srlmpetnvq gsnnslgkcq iyldldcntt etgtatndmk
      181 nisknvnkpd fqpsvklver ynlnnhteik fmvdtvinri iinvdgnvsn amirvpiasr
      241 ngretmitwt rkgyangvri dtrnpppgew wmklvgddna kyhfqvegvi eenppgklfs
      301 paprisgnng vititphrln ikrdpilnie eksddflktt dsevikaeqr nldarnnskl
      361 vddreitrer slkrrtflqe qneqatkeet nnsessvfis ntdnefinya eyvtfmdddg
      421 natipkkfdn gdqlfekmfs teeqekirar ssidavdnld idnseplkan nidisvrkie
      481 tldndelsee lvnyanqnti eekkmlievn rnsnllvtpg tihrivfdvm nncvlpvrya
      541 fqvkstpfkl ynvqplyawi ypgqmskvav dlivpnnaap dtantvtffi vgteikeksv
      601 ylyvqgsvsk ltddvkpkie ysfnnncagk lakdhcyksh wsidvtiedy dsglkrviss
      661 prniyprtef isgtrspvtf yysatccdts akitaidlld nyntytvdvt awnnlseaei
      721 aaitvgalla llliiliiii iiycfrrkks ldlpytqryg srppasaert nf
//
LOCUS       XP_034196426            1255 aa            linear   INV 03-SEP-2025
DEFINITION  structural maintenance of chromosomes 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196426
VERSION     XP_034196426.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340535.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1255
                     /product="structural maintenance of chromosomes 1 isoform
                     X1"
                     /calculated_mol_wt=144660
     Region          31..1230
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1255
                     /gene="SMC1"
                     /coded_by="XM_034340535.2:40..3807"
                     /db_xref="GeneID:117611974"
ORIGIN      
        1 mvfgiktlwr qlcyfavife yclitsrmpa flkhievenf ksykgkliig plksftavvg
       61 pngsgksnfm daisfvmgek tsslrvkrfs elihgasigm pvarsasvta vfeledgsek
      121 sfmrsvqgss sehrinnnvv tsqvylnele qlginvkakn flvfqgaves iamknpkert
      181 alfeeisnsg alkaeyerlr aemlkaeeet qfsyqkkkgi aaerkeakle keeaekyqrl
      241 keeyvekqve lqlfrlfhne kstenlevlq kkkqheieki ekkkekvdel lkekkkeagk
      301 lardlakieq direveveit kkrptfikak ervahmqkkv esarkslaqa riadeahkkd
      361 ihelqeelrq veeakaayea siagqsqlqg rdvqledeqv reynrlkeea gkqsarylql
      421 ldsinreqks dqdrldnegr kkteienkhk qkghmrdeal krvekleehi rtseaaledq
      481 kklradlqad vgtskdkiqn lqrelesise qlgdakvdkh evsrtkkkte ivenfkrlfp
      541 gvydrmynmc epihkrynva itkvlgkyme aivvdtekta rqciqylkeq ylepetflpl
      601 dyiqakplke rlrniqepkn vkllydvlhf spkdidravl fatnnalvce tpedankvay
      661 emdkkarydc valdgtfyqk agiisggsld lakkakrwde kqmsqlkaqk eklteelres
      721 lkksrkesel ntvesqirgl etrlkynksd liatqkqiae leaeletlqn elnmfgptia
      781 aiektmaerd qeiqnikekm nnveddvfas fceqigvsni rqyeerelrs qqerakkrme
      841 fenqcnriyn qldfekqrdt esnvlrwera vqdaedkles arqtesnqka eidhdetqme
      901 qlksarnakk mevdqkedei tkirrevaai akdiqsaqkq lniietkieq kkaerhailm
      961 qckmediaip mlhgnmedia testtsnsse vndsslsthq qyerekriti dyallpenlk
     1021 dideedvkkt tdkltktind lqntiqriqa pnmkaiqkly lakeklqetn eefeqsrkka
     1081 kkaktqfeki kkerhdrfma cfehvaneid piykslaknq saqaflgpen peepyldgin
     1141 yncvapgkrf qpmsnlsgge ktvaalallf aihsfqpapf fvldeidaal dntnigkvas
     1201 yirdktnslq iivislkeef yshadaligi cpdvdecles kvltldltay pkhsn
//
LOCUS       XP_034196427            1179 aa            linear   INV 03-SEP-2025
DEFINITION  structural maintenance of chromosomes 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196427
VERSION     XP_034196427.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340536.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1179
                     /product="structural maintenance of chromosomes 1 isoform
                     X2"
                     /calculated_mol_wt=136078
     Region          1..1154
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1179
                     /gene="SMC1"
                     /coded_by="XM_034340536.2:148..3687"
                     /db_xref="GeneID:117611974"
ORIGIN      
        1 mgektsslrv krfselihga sigmpvarsa svtavfeled gseksfmrsv qgsssehrin
       61 nnvvtsqvyl neleqlginv kaknflvfqg avesiamknp kertalfeei snsgalkaey
      121 erlraemlka eeetqfsyqk kkgiaaerke aklekeeaek yqrlkeeyve kqvelqlfrl
      181 fhnekstenl evlqkkkqhe iekiekkkek vdellkekkk eagklardla kieqdireve
      241 veitkkrptf ikakervahm qkkvesarks laqariadea hkkdihelqe elrqveeaka
      301 ayeasiagqs qlqgrdvqle deqvreynrl keeagkqsar ylqlldsinr eqksdqdrld
      361 negrkkteie nkhkqkghmr dealkrvekl eehirtseaa ledqkklrad lqadvgtskd
      421 kiqnlqrele siseqlgdak vdkhevsrtk kkteivenfk rlfpgvydrm ynmcepihkr
      481 ynvaitkvlg kymeaivvdt ektarqciqy lkeqylepet flpldyiqak plkerlrniq
      541 epknvkllyd vlhfspkdid ravlfatnna lvcetpedan kvayemdkka rydcvaldgt
      601 fyqkagiisg gsldlakkak rwdekqmsql kaqkekltee lreslkksrk eselntvesq
      661 irgletrlky nksdliatqk qiaeleaele tlqnelnmfg ptiaaiektm aerdqeiqni
      721 kekmnnvedd vfasfceqig vsnirqyeer elrsqqerak krmefenqcn riynqldfek
      781 qrdtesnvlr weravqdaed klesarqtes nqkaeidhde tqmeqlksar nakkmevdqk
      841 edeitkirre vaaiakdiqs aqkqlniiet kieqkkaerh ailmqckmed iaipmlhgnm
      901 ediatestts nssevndssl sthqqyerek ritidyallp enlkdideed vkkttdkltk
      961 tindlqntiq riqapnmkai qklylakekl qetneefeqs rkkakkaktq fekikkerhd
     1021 rfmacfehva neidpiyksl aknqsaqafl gpenpeepyl dginyncvap gkrfqpmsnl
     1081 sggektvaal allfaihsfq papffvldei daaldntnig kvasyirdkt nslqiivisl
     1141 keefyshada ligicpdvde cleskvltld ltaypkhsn
//
LOCUS       XP_034196428             826 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611975 [Osmia lignaria lignaria].
ACCESSION   XP_034196428
VERSION     XP_034196428.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340537.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034196428.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..826
                     /product="uncharacterized protein LOC117611975"
                     /calculated_mol_wt=94568
     CDS             1..826
                     /gene="LOC117611975"
                     /coded_by="XM_034340537.2:163..2643"
                     /db_xref="GeneID:117611975"
ORIGIN      
        1 mcimekdrkr kfdsgilsns yregigrvlg vkghmtyelp petikerqif wenffleyse
       61 qlsddiefdv ktfelcsrky qspavdkrks ivqhwvntsy lpyqtsihki gylennhfsi
      121 erglnmsytk phcnesflss svdtaayils ntnttnkadt mvivegakhd nlenvsdity
      181 sesteiskia dimhenngen ntrdnnnsnr rqhspvttei mtctvpqqni dtqenvdmld
      241 qalntkspsp dncesysckl snkvstpehs kesylrrsll dcsaikirhr kklytgrdsp
      301 vdlitmehsh kevlckltqh ihpalnfkyp lqkkrnirkk rsfkhwmvnk slmnstlsis
      361 dskksdeven irnysnrlss klydlkkldd apcanreils nsnntqdlnk csenqsinms
      421 rssqpvsrnh krdsknneln dkiiqsekvn trasdtfsnf kqstssnsvi rtvdsrkhkk
      481 ksnkimndpk nlnpvialtr lsnldilkyk klknyshnlh pvvclkrlse seiqkykrpt
      541 nmmkdkkyln ttvslktlse atikryktah nmktrsktdl lsdtvklsds smnrsqlslr
      601 nkanglksps niinvgscdt dsnqstilic kcnsktsdna slsnvvrssk klaefsspvv
      661 etcsnssrlk lseinknfnl rkditdrqsi dnilntsins qnyicnrsss rksktskngy
      721 sdnvhttidk klnpkhrrtn reklnvtkeq dengrnnlie ggicntivlg khksekqavv
      781 knliktlyng ktkknknkfn nanrlhfetr lydsdselts dsefhs
//
LOCUS       XP_034196435             312 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyl-protein thioesterase 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196435
VERSION     XP_034196435.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340544.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="palmitoyl-protein thioesterase 1 isoform X1"
                     /calculated_mol_wt=35900
     Region          29..284
                     /region_name="Palm_thioest"
                     /note="Palmitoyl protein thioesterase; pfam02089"
                     /db_xref="CDD:460441"
     CDS             1..312
                     /gene="Ppt1"
                     /coded_by="XM_034340544.2:460..1398"
                     /db_xref="GeneID:117611978"
ORIGIN      
        1 mftsrkttll lffflsiykl ngiqssslpv vlwhgmgdsc cfsfslgalk klieatvpni
       61 yvysirignn eiedvehsyf gninqdiqei cqrlsadkrf kdgynaigfs qgaqflrali
      121 qrcpnppikn fisvggqhqg vfglpicgtv kksvcnyvnr mikygaylkf vqekfiqaty
      181 whdpyqeeey kekstfmadi nneryinety kenlqklnam vlikfandti vwpretewfg
      241 fykpgqatei qtfeetdlyr kdqlglkall esgkihflsv tgnhlqfaes wfidhiikay
      301 limkkqavsn vt
//
LOCUS       XP_034196436             303 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyl-protein thioesterase 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196436
VERSION     XP_034196436.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340545.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="palmitoyl-protein thioesterase 1 isoform X2"
                     /calculated_mol_wt=34912
     Region          29..284
                     /region_name="Palm_thioest"
                     /note="Palmitoyl protein thioesterase; pfam02089"
                     /db_xref="CDD:460441"
     CDS             1..303
                     /gene="Ppt1"
                     /coded_by="XM_034340545.2:450..1361"
                     /db_xref="GeneID:117611978"
ORIGIN      
        1 mftsrkttll lffflsiykl ngiqssslpv vlwhgmgdsc cfsfslgalk klieatvpni
       61 yvysirignn eiedvehsyf gninqdiqei cqrlsadkrf kdgynaigfs qgaqflrali
      121 qrcpnppikn fisvggqhqg vfglpicgtv kksvcnyvnr mikygaylkf vqekfiqaty
      181 whdpyqeeey kekstfmadi nneryinety kenlqklnam vlikfandti vwpretewfg
      241 fykpgqatei qtfeetdlyr kdqlglkall esgkihflsv tgnhlqfaes wfidhiikay
      301 liv
//
LOCUS       XP_076546845             291 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyl-protein thioesterase 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546845
VERSION     XP_076546845.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690730.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="palmitoyl-protein thioesterase 1 isoform X3"
                     /calculated_mol_wt=34309
     Region          44..263
                     /region_name="Palm_thioest"
                     /note="Palmitoyl protein thioesterase; pfam02089"
                     /db_xref="CDD:460441"
     CDS             1..291
                     /gene="Ppt1"
                     /coded_by="XM_076690730.1:1..876"
                     /db_xref="GeneID:117611978"
ORIGIN      
        1 mqitnhflrk kkvayrlpkl hylddyfqve rrchrylqek riyvnvfnif lsdvehsyfg
       61 ninqdiqeic qrlsadkrfk dgynaigfsq gaqflraliq rcpnppiknf isvggqhqgv
      121 fglpicgtvk ksvcnyvnrm ikygaylkfv qekfiqatyw hdpyqeeeyk ekstfmadin
      181 neryinetyk enlqklnamv likfandtiv wpretewfgf ykpgqateiq tfeetdlyrk
      241 dqlglkalle sgkihflsvt gnhlqfaesw fidhiikayl imkkqavsnv t
//
LOCUS       XP_034196438             265 aa            linear   INV 03-SEP-2025
DEFINITION  thiol S-methyltransferase TMT1A-like [Osmia lignaria lignaria].
ACCESSION   XP_034196438
VERSION     XP_034196438.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340547.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..265
                     /product="thiol S-methyltransferase TMT1A-like"
                     /calculated_mol_wt=29741
     Region          84..202
                     /region_name="UbiE"
                     /note="Ubiquinone/menaquinone biosynthesis C-methylase
                     UbiE/MenG [Coenzyme transport and metabolism]; COG2226"
                     /db_xref="CDD:441828"
     CDS             1..265
                     /gene="LOC117611980"
                     /coded_by="XM_034340547.2:1..798"
                     /db_xref="GeneID:117611980"
ORIGIN      
        1 msnsdlwird iitsyglssl llvlltiwis rkwsdlqkti ykayltgfev ecselatsyk
       61 khlfkslqhi vssdnvlrsm ncirlleigv ktgeniqfyp dnthligvdr nvrlaeylik
      121 gnrswqfshi iierviigdg sslkdvptgy vdvvvtirsl csvtsiqstl qeirrvlapg
      181 gqylfiehvp anegtfiryl qkilsqtgiw pslfggchld idpithikna gfdnvisatf
      241 tldgyvsqsf hlalsrqhvl gvavr
//
LOCUS       XP_076546846            1596 aa            linear   INV 03-SEP-2025
DEFINITION  slowpoke 2 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076546846
VERSION     XP_076546846.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690731.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1596
                     /product="slowpoke 2 isoform X1"
                     /calculated_mol_wt=177513
     Region          255..422
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          <378..422
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          584..678
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1596
                     /gene="SLO2"
                     /coded_by="XM_076690731.1:180..4970"
                     /db_xref="GeneID:117611969"
ORIGIN      
        1 mlffksvpiv dlgesrgasg esprcsvtav svneeesyrk rnrlmalnsa gqvysmlrgg
       61 flrigwhwlq emsmyffrlh nlrrqaeevr kkrqkgrsnq sgqrgtnctd qsqptagsmh
      121 ccnrvhrgps rlferaplqp sdsldeiafq iprvrveyyv nentfkerlq lyfiknqrss
      181 lrirlanlff klltcflyif rvitdtdpty aacygctpgn kteflasqnl teeefqehpt
      241 inwdailwvr rplelwvvqv ilaivsltea lllaylgykg nvwqqllsfh filelvntip
      301 ftitllyppm rnlfipvfln cwlakhslen mfndlhramq ksqsalsqql milsatllcl
      361 vftsvcgiqh fqraghrhln lfqstyyvvv tfstvgygdf vpdiwpsqly mvimicvali
      421 vlptqfeqla ftwmerqklg gsysshraqs ekhvvvcstt lqadtimdfl nefyahpllq
      481 dyyvvllspm eldttmrmil qvpiwaqrvi yiqgsclkds dltrarmnea eacfilaarn
      541 yadktaadeh tilrswavkd fapnvpqyvq ifrpenklhv kfaehvvced efkyallann
      601 ctcpgastlv tlllhtsrgq egqqsqeewh rlygkcsgne iyhiilgdsr ffgeyegksf
      661 tyasfhshrk ygvalvavkp aelpefyeds illnpgprhi mkktdicyym sitkeensaf
      721 vvannqnvav egtqvvvetk tdgvgnanss sasntttnnt tttmnsgsga gtttttttis
      781 tsctiaggdg naccrqettc tgprgqsvhg tpahrapqvi lqvlpstptp lehhnilasd
      841 vhptyldpgg fdesrqclkd ggstpqtpit gnhldvprsm dpnpnllspe iltqrrgsrr
      901 psilpvpdml tssalnlpgq esldheades edemdddvpw rspsekiaiv kgfppvspyi
      961 gvsptfcyll kekkplcclq laqvcqhcsy rnakdynwqn ktiilaadya sngiynfiip
     1021 lrahfrskts lnpiilller tpeiafldav syfplvywmr gtidclddll ragitlaenv
     1081 vvvnkelsns aeedtladcn tivavqtmfk ffpsiksite lsqssnmrfm qfrahdkyal
     1141 hlskmekygr qleayflrek ergshisymf rlpfaagsvf sasmldtlly qafvkdymit
     1201 fvrlllgvdq apgsgfltsm ritkedmwir tygrlyqklc sttceipigi yrtqdttvsd
     1261 tshgdsdaes dagvgvtykv rhsaaascla ncatrdkgas aysvslgdea rdnhaqkier
     1321 aeiaslvrsr meslnlpvad ydvvsekrns lsyviinpsc dlkleegdiv ylvrpspfsa
     1381 qktferhnsr rksnisfcsa qlvsqmsaav asaglpagga gsrrgsgigl prapplgttk
     1441 snslslpdsp tvagtlrgrs nslrvvddil lrrsnslrqg ltmsrrkssl ediglgalsh
     1501 psnhnpikia lngsiglevt ppeegsqtgg asntgildvg lpsmpelraa lgsnigpglg
     1561 gslgglydpp slqgplqspq iqgstqdpqh iqgtiv
//
LOCUS       XP_076546847            1591 aa            linear   INV 03-SEP-2025
DEFINITION  slowpoke 2 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546847
VERSION     XP_076546847.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690732.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1591
                     /product="slowpoke 2 isoform X2"
                     /calculated_mol_wt=176928
     Region          255..417
                     /region_name="Ion_trans"
                     /note="Ion transport protein; pfam00520"
                     /db_xref="CDD:459842"
     Region          <373..417
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          579..673
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1591
                     /gene="SLO2"
                     /coded_by="XM_076690732.1:180..4955"
                     /db_xref="GeneID:117611969"
ORIGIN      
        1 mlffksvpiv dlgesrgasg esprcsvtav svneeesyrk rnrlmalnsa gqvysmlrgg
       61 flrigwhwlq emsmyffrlh nlrrqaeevr kkrqkgrsnq sgqrgtnctd qsqptagsmh
      121 ccnrvhrgps rlferaplqp sdsldeiafq iprvrveyyv nentfkerlq lyfiknqrss
      181 lrirlanlff klltcflyif rvitdtdpty aacygctpgn kteflasqnl teeefqehpt
      241 inwdailwvr rplelwvvqv ilaivsltea lllaylgykq llsfhfilel vntipftitl
      301 lyppmrnlfi pvflncwlak hslenmfndl hramqksqsa lsqqlmilsa tllclvftsv
      361 cgiqhfqrag hrhlnlfqst yyvvvtfstv gygdfvpdiw psqlymvimi cvalivlptq
      421 feqlaftwme rqklggsyss hraqsekhvv vcsttlqadt imdflnefya hpllqdyyvv
      481 llspmeldtt mrmilqvpiw aqrviyiqgs clkdsdltra rmneaeacfi laarnyadkt
      541 aadehtilrs wavkdfapnv pqyvqifrpe nklhvkfaeh vvcedefkya llannctcpg
      601 astlvtlllh tsrgqegqqs qeewhrlygk csgneiyhii lgdsrffgey egksftyasf
      661 hshrkygval vavkpaelpe fyedsillnp gprhimkktd icyymsitke ensafvvann
      721 qnvavegtqv vvetktdgvg nansssasnt ttnnttttmn sgsgagtttt tttistscti
      781 aggdgnaccr qettctgprg qsvhgtpahr apqvilqvlp stptplehhn ilasdvhpty
      841 ldpggfdesr qclkdggstp qtpitgnhld vprsmdpnpn llspeiltqr rgsrrpsilp
      901 vpdmltssal nlpgqesldh eadesedemd ddvpwrspse kiaivkgfpp vspyigvspt
      961 fcyllkekkp lcclqlaqvc qhcsyrnakd ynwqnktiil aadyasngiy nfiiplrahf
     1021 rsktslnpii lllertpeia fldavsyfpl vywmrgtidc lddllragit laenvvvvnk
     1081 elsnsaeedt ladcntivav qtmfkffpsi ksitelsqss nmrfmqfrah dkyalhlskm
     1141 ekygrqleay flrekergsh isymfrlpfa agsvfsasml dtllyqafvk dymitfvrll
     1201 lgvdqapgsg fltsmritke dmwirtygrl yqklcsttce ipigiyrtqd ttvsdtshgd
     1261 sdaesdagvg vtykvrhsaa asclancatr dkgasaysvs lgdeardnha qkieraeias
     1321 lvrsrmesln lpvadydvvs ekrnslsyvi inpscdlkle egdivylvrp spfsaqktfe
     1381 rhnsrrksni sfcsaqlvsq msaavasagl paggagsrrg sgiglprapp lgttksnsls
     1441 lpdsptvagt lrgrsnslrv vddillrrsn slrqgltmsr rkssledigl galshpsnhn
     1501 pikialngsi glevtppeeg sqtggasntg ildvglpsmp elraalgsni gpglggslgg
     1561 lydppslqgp lqspqiqgst qdpqhiqgti v
//
LOCUS       XP_076546848            1590 aa            linear   INV 03-SEP-2025
DEFINITION  slowpoke 2 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546848
VERSION     XP_076546848.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690733.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1590
                     /product="slowpoke 2 isoform X3"
                     /calculated_mol_wt=176800
     Region          <372..416
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          578..672
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1590
                     /gene="SLO2"
                     /coded_by="XM_076690733.1:180..4952"
                     /db_xref="GeneID:117611969"
ORIGIN      
        1 mlffksvpiv dlgesrgasg esprcsvtav svneeesyrk rnrlmalnsa gqvysmlrgg
       61 flrigwhwlq emsmyffrlh nlrrqaeevr kkrqkgrsnq sgqrgtnctd qsqptagsmh
      121 ccnrvhrgps rlferaplqp sdsldeiafq iprvrveyyv nentfkerlq lyfiknqrss
      181 lrirlanlff klltcflyif rvitdtdpty aacygctpgn kteflasqnl teeefqehpt
      241 inwdailwvr rplelwvvqv ilaivsltea lllaylgykl lsfhfilelv ntipftitll
      301 yppmrnlfip vflncwlakh slenmfndlh ramqksqsal sqqlmilsat llclvftsvc
      361 giqhfqragh rhlnlfqsty yvvvtfstvg ygdfvpdiwp sqlymvimic valivlptqf
      421 eqlaftwmer qklggsyssh raqsekhvvv csttlqadti mdflnefyah pllqdyyvvl
      481 lspmeldttm rmilqvpiwa qrviyiqgsc lkdsdltrar mneaeacfil aarnyadkta
      541 adehtilrsw avkdfapnvp qyvqifrpen klhvkfaehv vcedefkyal lannctcpga
      601 stlvtlllht srgqegqqsq eewhrlygkc sgneiyhiil gdsrffgeye gksftyasfh
      661 shrkygvalv avkpaelpef yedsillnpg prhimkktdi cyymsitkee nsafvvannq
      721 nvavegtqvv vetktdgvgn ansssasntt tnnttttmns gsgagttttt ttistsctia
      781 ggdgnaccrq ettctgprgq svhgtpahra pqvilqvlps tptplehhni lasdvhptyl
      841 dpggfdesrq clkdggstpq tpitgnhldv prsmdpnpnl lspeiltqrr gsrrpsilpv
      901 pdmltssaln lpgqesldhe adesedemdd dvpwrspsek iaivkgfppv spyigvsptf
      961 cyllkekkpl cclqlaqvcq hcsyrnakdy nwqnktiila adyasngiyn fiiplrahfr
     1021 sktslnpiil llertpeiaf ldavsyfplv ywmrgtidcl ddllragitl aenvvvvnke
     1081 lsnsaeedtl adcntivavq tmfkffpsik sitelsqssn mrfmqfrahd kyalhlskme
     1141 kygrqleayf lrekergshi symfrlpfaa gsvfsasmld tllyqafvkd ymitfvrlll
     1201 gvdqapgsgf ltsmritked mwirtygrly qklcsttcei pigiyrtqdt tvsdtshgds
     1261 daesdagvgv tykvrhsaaa sclancatrd kgasaysvsl gdeardnhaq kieraeiasl
     1321 vrsrmeslnl pvadydvvse krnslsyvii npscdlklee gdivylvrps pfsaqktfer
     1381 hnsrrksnis fcsaqlvsqm saavasaglp aggagsrrgs giglprappl gttksnslsl
     1441 pdsptvagtl rgrsnslrvv ddillrrsns lrqgltmsrr ksslediglg alshpsnhnp
     1501 ikialngsig levtppeegs qtggasntgi ldvglpsmpe lraalgsnig pglggslggl
     1561 ydppslqgpl qspqiqgstq dpqhiqgtiv
//
LOCUS       XP_076546849            1545 aa            linear   INV 03-SEP-2025
DEFINITION  slowpoke 2 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076546849
VERSION     XP_076546849.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690734.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1545
                     /product="slowpoke 2 isoform X4"
                     /calculated_mol_wt=172042
     Region          <378..422
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          584..678
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1545
                     /gene="SLO2"
                     /coded_by="XM_076690734.1:180..4817"
                     /db_xref="GeneID:117611969"
ORIGIN      
        1 mlffksvpiv dlgesrgasg esprcsvtav svneeesyrk rnrlmalnsa gqvysmlrgg
       61 flrigwhwlq emsmyffrlh nlrrqaeevr kkrqkgrsnq sgqrgtnctd qsqptagsmh
      121 ccnrvhrgps rlferaplqp sdsldeiafq iprvrveyyv nentfkerlq lyfiknqrss
      181 lrirlanlff klltcflyif rvitdtdpty aacygctpgn kteflasqnl teeefqehpt
      241 inwdailwvr rplelwvvqv ilaivsltea lllaylgykg nvwqqllsfh filelvntip
      301 ftitllyppm rnlfipvfln cwlakhslen mfndlhramq ksqsalsqql milsatllcl
      361 vftsvcgiqh fqraghrhln lfqstyyvvv tfstvgygdf vpdiwpsqly mvimicvali
      421 vlptqfeqla ftwmerqklg gsysshraqs ekhvvvcstt lqadtimdfl nefyahpllq
      481 dyyvvllspm eldttmrmil qvpiwaqrvi yiqgsclkds dltrarmnea eacfilaarn
      541 yadktaadeh tilrswavkd fapnvpqyvq ifrpenklhv kfaehvvced efkyallann
      601 ctcpgastlv tlllhtsrgq egqqsqeewh rlygkcsgne iyhiilgdsr ffgeyegksf
      661 tyasfhshrk ygvalvavkp aelpefyeds illnpgprhi mkktdicyym sitkeensaf
      721 vvannqnvav egtqvvvetk tdgvgnanss sasntttnnt tttmnsgsga gtttttttis
      781 tsctiaggdg natyldpggf desrqclkdg gstpqtpitg nhldvprsmd pnpnllspei
      841 ltqrrgsrrp silpvpdmlt ssalnlpgqe sldheadese demdddvpwr spsekiaivk
      901 gfppvspyig vsptfcyllk ekkplcclql aqvcqhcsyr nakdynwqnk tiilaadyas
      961 ngiynfiipl rahfrsktsl npiilllert peiafldavs yfplvywmrg tidclddllr
     1021 agitlaenvv vvnkelsnsa eedtladcnt ivavqtmfkf fpsiksitel sqssnmrfmq
     1081 frahdkyalh lskmekygrq leayflreke rgshisymfr lpfaagsvfs asmldtllyq
     1141 afvkdymitf vrlllgvdqa pgsgfltsmr itkedmwirt ygrlyqklcs ttceipigiy
     1201 rtqdttvsdt shgdsdaesd agvgvtykvr hsaaasclan catrdkgasa ysvslgdear
     1261 dnhaqkiera eiaslvrsrm eslnlpvady dvvsekrnsl syviinpscd lkleegdivy
     1321 lvrpspfsaq ktferhnsrr ksnisfcsaq lvsqmsaava saglpaggag srrgsgiglp
     1381 rapplgttks nslslpdspt vagtlrgrsn slrvvddill rrsnslrqgl tmsrrkssle
     1441 diglgalshp snhnpikial ngsiglevtp peegsqtgga sntgildvgl psmpelraal
     1501 gsnigpglgg slgglydpps lqgplqspqi qgstqdpqhi qgtiv
//
LOCUS       XP_076546850            1477 aa            linear   INV 03-SEP-2025
DEFINITION  slowpoke 2 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076546850
VERSION     XP_076546850.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690735.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1477
                     /product="slowpoke 2 isoform X5"
                     /calculated_mol_wt=163969
     Region          <259..303
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          465..559
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1477
                     /gene="SLO2"
                     /coded_by="XM_076690735.1:782..5215"
                     /db_xref="GeneID:117611969"
ORIGIN      
        1 mnepdpclpl ilppryrlrd lilgdyaftd dgervrveyy vnentfkerl qlyfiknqrs
       61 slrirlanlf fklltcflyi frvitdtdpt yaacygctpg nkteflasqn lteeefqehp
      121 tinwdailwv rrplelwvvq vilaivslte alllaylgyk gnvwqqllsf hfilelvnti
      181 pftitllypp mrnlfipvfl ncwlakhsle nmfndlhram qksqsalsqq lmilsatllc
      241 lvftsvcgiq hfqraghrhl nlfqstyyvv vtfstvgygd fvpdiwpsql ymvimicval
      301 ivlptqfeql aftwmerqkl ggsysshraq sekhvvvcst tlqadtimdf lnefyahpll
      361 qdyyvvllsp meldttmrmi lqvpiwaqrv iyiqgsclkd sdltrarmne aeacfilaar
      421 nyadktaade htilrswavk dfapnvpqyv qifrpenklh vkfaehvvce defkyallan
      481 nctcpgastl vtlllhtsrg qegqqsqeew hrlygkcsgn eiyhiilgds rffgeyegks
      541 ftyasfhshr kygvalvavk paelpefyed sillnpgprh imkktdicyy msitkeensa
      601 fvvannqnva vegtqvvvet ktdgvgnans ssasntttnn ttttmnsgsg agttttttti
      661 stsctiaggd gnaccrqett ctgprgqsvh gtpahrapqv ilqvlpstpt plehhnilas
      721 dvhptyldpg gfdesrqclk dggstpqtpi tgnhldvprs mdpnpnllsp eiltqrrgsr
      781 rpsilpvpdm ltssalnlpg qesldheade sedemdddvp wrspsekiai vkgfppvspy
      841 igvsptfcyl lkekkplccl qlaqvcqhcs yrnakdynwq nktiilaady asngiynfii
      901 plrahfrskt slnpiillle rtpeiaflda vsyfplvywm rgtidclddl lragitlaen
      961 vvvvnkelsn saeedtladc ntivavqtmf kffpsiksit elsqssnmrf mqfrahdkya
     1021 lhlskmekyg rqleayflre kergshisym frlpfaagsv fsasmldtll yqafvkdymi
     1081 tfvrlllgvd qapgsgflts mritkedmwi rtygrlyqkl csttceipig iyrtqdttvs
     1141 dtshgdsdae sdagvgvtyk vrhsaaascl ancatrdkga saysvslgde ardnhaqkie
     1201 raeiaslvrs rmeslnlpva dydvvsekrn slsyviinps cdlkleegdi vylvrpspfs
     1261 aqktferhns rrksnisfcs aqlvsqmsaa vasaglpagg agsrrgsgig lprapplgtt
     1321 ksnslslpds ptvagtlrgr snslrvvddi llrrsnslrq gltmsrrkss lediglgals
     1381 hpsnhnpiki alngsiglev tppeegsqtg gasntgildv glpsmpelra algsnigpgl
     1441 ggslgglydp pslqgplqsp qiqgstqdpq hiqgtiv
//
LOCUS       XP_076546851            1467 aa            linear   INV 03-SEP-2025
DEFINITION  slowpoke 2 isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076546851
VERSION     XP_076546851.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690736.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1467
                     /product="slowpoke 2 isoform X6"
                     /calculated_mol_wt=162883
     Region          <249..293
                     /region_name="Ion_trans_2"
                     /note="Ion channel; pfam07885"
                     /db_xref="CDD:462301"
     Region          455..549
                     /region_name="BK_channel_a"
                     /note="Calcium-activated BK potassium channel alpha
                     subunit; pfam03493"
                     /db_xref="CDD:460947"
     CDS             1..1467
                     /gene="SLO2"
                     /coded_by="XM_076690736.1:1543..5946"
                     /db_xref="GeneID:117611969"
ORIGIN      
        1 mttssryssy ncltswdsyd riprvrveyy vnentfkerl qlyfiknqrs slrirlanlf
       61 fklltcflyi frvitdtdpt yaacygctpg nkteflasqn lteeefqehp tinwdailwv
      121 rrplelwvvq vilaivslte alllaylgyk gnvwqqllsf hfilelvnti pftitllypp
      181 mrnlfipvfl ncwlakhsle nmfndlhram qksqsalsqq lmilsatllc lvftsvcgiq
      241 hfqraghrhl nlfqstyyvv vtfstvgygd fvpdiwpsql ymvimicval ivlptqfeql
      301 aftwmerqkl ggsysshraq sekhvvvcst tlqadtimdf lnefyahpll qdyyvvllsp
      361 meldttmrmi lqvpiwaqrv iyiqgsclkd sdltrarmne aeacfilaar nyadktaade
      421 htilrswavk dfapnvpqyv qifrpenklh vkfaehvvce defkyallan nctcpgastl
      481 vtlllhtsrg qegqqsqeew hrlygkcsgn eiyhiilgds rffgeyegks ftyasfhshr
      541 kygvalvavk paelpefyed sillnpgprh imkktdicyy msitkeensa fvvannqnva
      601 vegtqvvvet ktdgvgnans ssasntttnn ttttmnsgsg agttttttti stsctiaggd
      661 gnaccrqett ctgprgqsvh gtpahrapqv ilqvlpstpt plehhnilas dvhptyldpg
      721 gfdesrqclk dggstpqtpi tgnhldvprs mdpnpnllsp eiltqrrgsr rpsilpvpdm
      781 ltssalnlpg qesldheade sedemdddvp wrspsekiai vkgfppvspy igvsptfcyl
      841 lkekkplccl qlaqvcqhcs yrnakdynwq nktiilaady asngiynfii plrahfrskt
      901 slnpiillle rtpeiaflda vsyfplvywm rgtidclddl lragitlaen vvvvnkelsn
      961 saeedtladc ntivavqtmf kffpsiksit elsqssnmrf mqfrahdkya lhlskmekyg
     1021 rqleayflre kergshisym frlpfaagsv fsasmldtll yqafvkdymi tfvrlllgvd
     1081 qapgsgflts mritkedmwi rtygrlyqkl csttceipig iyrtqdttvs dtshgdsdae
     1141 sdagvgvtyk vrhsaaascl ancatrdkga saysvslgde ardnhaqkie raeiaslvrs
     1201 rmeslnlpva dydvvsekrn slsyviinps cdlkleegdi vylvrpspfs aqktferhns
     1261 rrksnisfcs aqlvsqmsaa vasaglpagg agsrrgsgig lprapplgtt ksnslslpds
     1321 ptvagtlrgr snslrvvddi llrrsnslrq gltmsrrkss lediglgals hpsnhnpiki
     1381 alngsiglev tppeegsqtg gasntgildv glpsmpelra algsnigpgl ggslgglydp
     1441 pslqgplqsp qiqgstqdpq hiqgtiv
//
LOCUS       XP_034196415             548 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196415
VERSION     XP_034196415.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340524.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..548
                     /product="uncharacterized protein LOC117611970 isoform X1"
                     /calculated_mol_wt=60898
     Region          232..365
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          402..532
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..548
                     /gene="LOC117611970"
                     /coded_by="XM_034340524.2:116..1762"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 msssefneei kqkvpseevs ndedsddsle yfweevenls lesprvggpl sqslamippt
       61 mhghefnqpl srvvmvrktd qrtfskgrrg geplletvtr etvelfnggr aerkytsetr
      121 divtpksnsm pslsvngtkk kvvgfvdqls persrtdsvr skspltaspa spgplsnslh
      181 gsfhgslgsd gmscssarss saspdsarys stpitktdtr kpsvrrlgpp refisvclet
      241 hncyrarhgv pplrlskqlc ktsqdwanil aargrlehra nsdygenlyc mwssnpktvv
      301 sgdepvnewy aeeaqhqygk epttlktghf tqvvwrdste lgvgmarnrn gevyvvcnyn
      361 pagnflgsft envlppgcss ptkkitfldp kqqypldeqa wqqeallvhn eyrrrhrvpd
      421 ltlsadltaa akawantlln tnklipqsss pygeniysmq csdpklivpa revvskwyse
      481 kkdhkfgtep kvlntchftq ivwknttemg iamakkdgtc vivacyhprg nivgqftenv
      541 lkpvktac
//
LOCUS       XP_034196416             524 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196416
VERSION     XP_034196416.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340525.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..524
                     /product="uncharacterized protein LOC117611970 isoform X2"
                     /calculated_mol_wt=58368
     Region          208..341
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          378..508
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..524
                     /gene="LOC117611970"
                     /coded_by="XM_034340525.2:116..1690"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 msssefneei kqkvpseevs ndedsddsle yfweevenls lesprvggpl sqslamippt
       61 mhghefnqpl srvvmvrktd qrtfskgrrg geplletvtr etvelfnggr aerkytsetr
      121 divtpksnrs rtdsvrsksp ltaspaspgp lsnslhgsfh gslgsdgmsc ssarsssasp
      181 dsarysstpi tktdtrkpsv rrlgpprefi svclethncy rarhgvpplr lskqlcktsq
      241 dwanilaarg rlehransdy genlycmwss npktvvsgde pvnewyaeea qhqygkeptt
      301 lktghftqvv wrdstelgvg marnrngevy vvcnynpagn flgsftenvl ppgcssptkk
      361 itfldpkqqy pldeqawqqe allvhneyrr rhrvpdltls adltaaakaw antllntnkl
      421 ipqssspyge niysmqcsdp klivparevv skwysekkdh kfgtepkvln tchftqivwk
      481 nttemgiama kkdgtcviva cyhprgnivg qftenvlkpv ktac
//
LOCUS       XP_034196417             518 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196417
VERSION     XP_034196417.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340526.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..518
                     /product="uncharacterized protein LOC117611970 isoform X3"
                     /calculated_mol_wt=57322
     Region          202..335
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          372..502
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..518
                     /gene="LOC117611970"
                     /coded_by="XM_034340526.2:1478..3034"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 mtskpgrsys vrsprvggpl sqslamippt mhghefnqpl srvvmvrktd qrtfskgrrg
       61 geplletvtr etvelfnggr aerkytsetr divtpksnsm pslsvngtkk kvvgfvdqls
      121 persrtdsvr skspltaspa spgplsnslh gsfhgslgsd gmscssarss saspdsarys
      181 stpitktdtr kpsvrrlgpp refisvclet hncyrarhgv pplrlskqlc ktsqdwanil
      241 aargrlehra nsdygenlyc mwssnpktvv sgdepvnewy aeeaqhqygk epttlktghf
      301 tqvvwrdste lgvgmarnrn gevyvvcnyn pagnflgsft envlppgcss ptkkitfldp
      361 kqqypldeqa wqqeallvhn eyrrrhrvpd ltlsadltaa akawantlln tnklipqsss
      421 pygeniysmq csdpklivpa revvskwyse kkdhkfgtep kvlntchftq ivwknttemg
      481 iamakkdgtc vivacyhprg nivgqftenv lkpvktac
//
LOCUS       XP_034196418             518 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196418
VERSION     XP_034196418.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340527.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..518
                     /product="uncharacterized protein LOC117611970 isoform X3"
                     /calculated_mol_wt=57322
     Region          202..335
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          372..502
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..518
                     /gene="LOC117611970"
                     /coded_by="XM_034340527.2:1131..2687"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 mtskpgrsys vrsprvggpl sqslamippt mhghefnqpl srvvmvrktd qrtfskgrrg
       61 geplletvtr etvelfnggr aerkytsetr divtpksnsm pslsvngtkk kvvgfvdqls
      121 persrtdsvr skspltaspa spgplsnslh gsfhgslgsd gmscssarss saspdsarys
      181 stpitktdtr kpsvrrlgpp refisvclet hncyrarhgv pplrlskqlc ktsqdwanil
      241 aargrlehra nsdygenlyc mwssnpktvv sgdepvnewy aeeaqhqygk epttlktghf
      301 tqvvwrdste lgvgmarnrn gevyvvcnyn pagnflgsft envlppgcss ptkkitfldp
      361 kqqypldeqa wqqeallvhn eyrrrhrvpd ltlsadltaa akawantlln tnklipqsss
      421 pygeniysmq csdpklivpa revvskwyse kkdhkfgtep kvlntchftq ivwknttemg
      481 iamakkdgtc vivacyhprg nivgqftenv lkpvktac
//
LOCUS       XP_034196420             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196420
VERSION     XP_034196420.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340529.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117611970 isoform X4"
                     /calculated_mol_wt=54721
     Region          177..310
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          347..477
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..493
                     /gene="LOC117611970"
                     /coded_by="XM_034340529.2:449..1930"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 mipptmhghe fnqplsrvvm vrktdqrtfs kgrrggepll etvtretvel fnggraerky
       61 tsetrdivtp ksnsmpslsv ngtkkkvvgf vdqlspersr tdsvrskspl taspaspgpl
      121 snslhgsfhg slgsdgmscs sarsssaspd sarysstpit ktdtrkpsvr rlgpprefis
      181 vclethncyr arhgvpplrl skqlcktsqd wanilaargr lehransdyg enlycmwssn
      241 pktvvsgdep vnewyaeeaq hqygkepttl ktghftqvvw rdstelgvgm arnrngevyv
      301 vcnynpagnf lgsftenvlp pgcssptkki tfldpkqqyp ldeqawqqea llvhneyrrr
      361 hrvpdltlsa dltaaakawa ntllntnkli pqssspygen iysmqcsdpk livparevvs
      421 kwysekkdhk fgtepkvlnt chftqivwkn ttemgiamak kdgtcvivac yhprgnivgq
      481 ftenvlkpvk tac
//
LOCUS       XP_034196422             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196422
VERSION     XP_034196422.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340531.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117611970 isoform X4"
                     /calculated_mol_wt=54721
     Region          177..310
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          347..477
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..493
                     /gene="LOC117611970"
                     /coded_by="XM_034340531.2:196..1677"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 mipptmhghe fnqplsrvvm vrktdqrtfs kgrrggepll etvtretvel fnggraerky
       61 tsetrdivtp ksnsmpslsv ngtkkkvvgf vdqlspersr tdsvrskspl taspaspgpl
      121 snslhgsfhg slgsdgmscs sarsssaspd sarysstpit ktdtrkpsvr rlgpprefis
      181 vclethncyr arhgvpplrl skqlcktsqd wanilaargr lehransdyg enlycmwssn
      241 pktvvsgdep vnewyaeeaq hqygkepttl ktghftqvvw rdstelgvgm arnrngevyv
      301 vcnynpagnf lgsftenvlp pgcssptkki tfldpkqqyp ldeqawqqea llvhneyrrr
      361 hrvpdltlsa dltaaakawa ntllntnkli pqssspygen iysmqcsdpk livparevvs
      421 kwysekkdhk fgtepkvlnt chftqivwkn ttemgiamak kdgtcvivac yhprgnivgq
      481 ftenvlkpvk tac
//
LOCUS       XP_034196421             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196421
VERSION     XP_034196421.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340530.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117611970 isoform X4"
                     /calculated_mol_wt=54721
     Region          177..310
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          347..477
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..493
                     /gene="LOC117611970"
                     /coded_by="XM_034340530.2:351..1832"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 mipptmhghe fnqplsrvvm vrktdqrtfs kgrrggepll etvtretvel fnggraerky
       61 tsetrdivtp ksnsmpslsv ngtkkkvvgf vdqlspersr tdsvrskspl taspaspgpl
      121 snslhgsfhg slgsdgmscs sarsssaspd sarysstpit ktdtrkpsvr rlgpprefis
      181 vclethncyr arhgvpplrl skqlcktsqd wanilaargr lehransdyg enlycmwssn
      241 pktvvsgdep vnewyaeeaq hqygkepttl ktghftqvvw rdstelgvgm arnrngevyv
      301 vcnynpagnf lgsftenvlp pgcssptkki tfldpkqqyp ldeqawqqea llvhneyrrr
      361 hrvpdltlsa dltaaakawa ntllntnkli pqssspygen iysmqcsdpk livparevvs
      421 kwysekkdhk fgtepkvlnt chftqivwkn ttemgiamak kdgtcvivac yhprgnivgq
      481 ftenvlkpvk tac
//
LOCUS       XP_076546852             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546852
VERSION     XP_076546852.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690737.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117611970 isoform X4"
                     /calculated_mol_wt=54721
     Region          177..310
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          347..477
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..493
                     /gene="LOC117611970"
                     /coded_by="XM_076690737.1:236..1717"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 mipptmhghe fnqplsrvvm vrktdqrtfs kgrrggepll etvtretvel fnggraerky
       61 tsetrdivtp ksnsmpslsv ngtkkkvvgf vdqlspersr tdsvrskspl taspaspgpl
      121 snslhgsfhg slgsdgmscs sarsssaspd sarysstpit ktdtrkpsvr rlgpprefis
      181 vclethncyr arhgvpplrl skqlcktsqd wanilaargr lehransdyg enlycmwssn
      241 pktvvsgdep vnewyaeeaq hqygkepttl ktghftqvvw rdstelgvgm arnrngevyv
      301 vcnynpagnf lgsftenvlp pgcssptkki tfldpkqqyp ldeqawqqea llvhneyrrr
      361 hrvpdltlsa dltaaakawa ntllntnkli pqssspygen iysmqcsdpk livparevvs
      421 kwysekkdhk fgtepkvlnt chftqivwkn ttemgiamak kdgtcvivac yhprgnivgq
      481 ftenvlkpvk tac
//
LOCUS       XP_034196423             493 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611970 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034196423
VERSION     XP_034196423.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034340532.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..493
                     /product="uncharacterized protein LOC117611970 isoform X4"
                     /calculated_mol_wt=54721
     Region          177..310
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     Region          347..477
                     /region_name="CAP_GAPR1-like"
                     /note="CAP (cysteine-rich secretory proteins, antigen 5,
                     and pathogenesis-related 1 proteins) domain of
                     Golgi-associated plant pathogenesis-related protein 1 and
                     similar proteins; cd05382"
                     /db_xref="CDD:349401"
     CDS             1..493
                     /gene="LOC117611970"
                     /coded_by="XM_034340532.2:65..1546"
                     /db_xref="GeneID:117611970"
ORIGIN      
        1 mipptmhghe fnqplsrvvm vrktdqrtfs kgrrggepll etvtretvel fnggraerky
       61 tsetrdivtp ksnsmpslsv ngtkkkvvgf vdqlspersr tdsvrskspl taspaspgpl
      121 snslhgsfhg slgsdgmscs sarsssaspd sarysstpit ktdtrkpsvr rlgpprefis
      181 vclethncyr arhgvpplrl skqlcktsqd wanilaargr lehransdyg enlycmwssn
      241 pktvvsgdep vnewyaeeaq hqygkepttl ktghftqvvw rdstelgvgm arnrngevyv
      301 vcnynpagnf lgsftenvlp pgcssptkki tfldpkqqyp ldeqawqqea llvhneyrrr
      361 hrvpdltlsa dltaaakawa ntllntnkli pqssspygen iysmqcsdpk livparevvs
      421 kwysekkdhk fgtepkvlnt chftqivwkn ttemgiamak kdgtcvivac yhprgnivgq
      481 ftenvlkpvk tac
//
LOCUS       XP_034191850            1312 aa            linear   INV 03-SEP-2025
DEFINITION  condensin-2 complex subunit D3-L [Osmia lignaria lignaria].
ACCESSION   XP_034191850
VERSION     XP_034191850.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335959.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191850.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1312
                     /product="condensin-2 complex subunit D3-L"
                     /calculated_mol_wt=150446
     Region          <486..>621
                     /region_name="COG5098"
                     /note="Chromosome condensation complex Condensin, subunit
                     D2 [Chromatin structure and dynamics / Cell division and
                     chromosome partitioning]"
                     /db_xref="CDD:227429"
     Region          487..515
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          524..550
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          562..588
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          878..>1030
                     /region_name="Cnd1"
                     /note="non-SMC mitotic condensation complex subunit 1;
                     pfam12717"
                     /db_xref="CDD:463677"
     CDS             1..1312
                     /gene="LOC117609515"
                     /coded_by="XM_034335959.2:456..4394"
                     /db_xref="GeneID:117609515"
ORIGIN      
        1 meslkifdnf kfseldedwi esvwngefmi fkeppdeyfm fiesedmrll lhdsytivka
       61 wlldrrsqnd setseiswnt liimdvkvra llavlgyiik sgqgketded srqsslyatn
      121 lyfillaipg ssafnvfhpn lyqqtietik thseqlqpct kkhskamsld dsvitdelmn
      181 enyvlshnek etlirnlnai istfiimtrs fwfkdhiqsl ditiqgllev tkiddasvvs
      241 qhnghrdsla aslpqnayla lhelcdnkhg pikvtatfia kyilprllcn hmdaqakvia
      301 nirkntihfl qdllhtyerd aetailtliq himlncpdrl darqkqasvl ikvleickhh
      361 imlealknii llshhskitf rlfaqeiigk lllepfimnc simdnlktki krvlfaivss
      421 rcmdcssmvr gkaiaiiaef testnaidka ifesifnesn pnkkflafhe lkdalsedie
      481 llpgsnvlit ilmerieder amirrstlqi lknlilmfpn likevlsvis rrcrdpallv
      541 rrfavqmlsq llqqfpdnsq lldewvqtvv pqifdmeikv qekvleylhe lllnrikdis
      601 tntdtnadnl pwkilntlan mkmrkhlska cnlwvkhgii tnsvvkniqs higtnnniga
      661 wvllvalaen tklpnmnkyi snykeilesn nfctslvlqv lryswksldy evleqlhvym
      721 ykclqqfkis yglisicldi ihniilylnp dkgnnllely menlikvset eitkifines
      781 qlvdivpnyl kamftlgnat llgaykisps ilrifqgmll eweslpdvik dinelqasav
      841 vilgqqavrd rdvakeilpi fgklmrqetn ldsiiqiavk intakaladi cvrftalvep
      901 ylsdmcvsmk dsspqvreai mvifiqllle dyikikgpff fhiltmlsds dsmireltnf
      961 likerlltkn ktlisqqflq siyhynnyrs qhkvydqkmr ekerkaltlp gkknedkrrv
     1021 iydfmldhld ppgkikllvk itsqilggtc idtinvnkee gvgvlkdaly iignkrlrps
     1081 sfnkhtdddq qegdepavqt itpttnaitv iiegikkhgl evllpmlikl rkklsllksp
     1141 lesdikklli ktyseynkdq lsnilneypq lekeveqfkk qlddtafnde dsseevmlps
     1201 etnkqitnnq nsdlttcslh skvvlqsipi srltpttsid cwnfstpsvs nlrllspaqp
     1261 gpststpnsf ksldfnghcs karrlssffq nekqlnnfhy tarevqydse sd
//
LOCUS       XP_034191865             376 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear speckle splicing regulatory protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191865
VERSION     XP_034191865.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335974.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191865.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="nuclear speckle splicing regulatory protein 1"
                     /calculated_mol_wt=43830
     Region          61..178
                     /region_name="NSRP1_N"
                     /note="Nuclear speckle splicing regulatory protein 1,
                     N-terminal; pfam09745"
                     /db_xref="CDD:462876"
     CDS             1..376
                     /gene="LOC117609520"
                     /coded_by="XM_034335974.2:176..1306"
                     /db_xref="GeneID:117609520"
ORIGIN      
        1 mfnstdekqy glilpkkqqs vaakvsnifg dnndsdeedg tdwvkktlka egeknkikkq
       61 tklniqkalk edptifqyde vyddmergkd qskaikdekk kpryiqnllk aaerrkkeqe
      121 yrvermvqke reaegamfad kesfvtsayr akleefkkme eeenetdrle aigdvkkqqd
      181 isgfyrhlye qtvksseece vtndvnndki nldhdstnvi itekdeikik evkknrqyrq
      241 riveedsdtd tetrqkeqdn kaktvlsekr kidekevdls eantkkqkqq tedsetdkvn
      301 npivvedisn vkdaekeeek cednskiika ditvkakiea ekrdrskiwe krtigpvfea
      361 alqryyarka trlsaa
//
LOCUS       XP_034191879             346 aa            linear   INV 03-SEP-2025
DEFINITION  lymphokine-activated killer T-cell-originated protein kinase [Osmia
            lignaria lignaria].
ACCESSION   XP_034191879
VERSION     XP_034191879.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191879.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..346
                     /product="lymphokine-activated killer T-cell-originated
                     protein kinase"
                     /calculated_mol_wt=39279
     Region          31..339
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(37..40,43,45,62,64,96,113..116,164,168..169,171,
                     182..183)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..346
                     /gene="LOC117609526"
                     /coded_by="XM_034335988.2:145..1185"
                     /db_xref="GeneID:117609526"
ORIGIN      
        1 mdlfktptnk kyrsrvkdnp elqtpikipa spfleqigyg cgvsvfnler spkvgfvrsp
       61 waikkrnrki qesekyneri rfeaeilrkl nhpnivgfra faemangepc lamekldasl
      121 gdkieeraen dddpfpakvi lkityeivkg lvylhhtahi lhgdiksfni lvsedynrvk
      181 lcdfgvsvpl teslvldtsk gdfryigtec wsapeivnet gpvtnkadiw tcgmviwemi
      241 alspphvqms dssleescsd esllemqgkk eekvdeqnis mddsvtffse iakygtrppl
      301 painlgkeyq nvleiffact cekyevrpsa kgldnyfknc iykiqk
//
LOCUS       XP_034191889             317 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin-1A isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191889
VERSION     XP_034191889.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335998.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191889.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="syntaxin-1A isoform X1"
                     /calculated_mol_wt=36813
     Region          39..258
                     /region_name="Syntaxin"
                     /note="pfam00804"
                     /db_xref="CDD:459942"
     Region          <236..284
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(254,261,264)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277192"
     Site            257
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277192"
     CDS             1..317
                     /gene="LOC117609533"
                     /coded_by="XM_034335998.2:660..1613"
                     /db_xref="GeneID:117609533"
ORIGIN      
        1 mvrdrmpelr acqnnsttfg kgflqdvhiq isqnkklkev lneveevral ievitenisi
       61 ikdlhnnvla ytnkgknkip nvvivevsin killsdmqke lenrtytisq tsfriqrklr
      121 emgkeiasid dltlssandg pihirikalq yttmlrlfse imeeynisml ryhekcrlll
      181 qqqkilirkr itseelekll dnqgnslfvd niledsriar qqlsdiqirh ndiikieksi
      241 tevrdmftem afliekqgdq lnsveyfagk tadnvdsgrt dlkkaeqhsh rhrkrkiklc
      301 iiisiiiivf lliiifv
//
LOCUS       XP_034191899             296 aa            linear   INV 03-SEP-2025
DEFINITION  syntaxin-1A isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191899
VERSION     XP_034191899.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336008.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191899.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..296
                     /product="syntaxin-1A isoform X2"
                     /calculated_mol_wt=34553
     Region          39..237
                     /region_name="Syntaxin"
                     /note="pfam00804"
                     /db_xref="CDD:459942"
     Region          <215..263
                     /region_name="SNARE"
                     /note="SNARE motif; cl22856"
                     /db_xref="CDD:473982"
     Site            order(233,240,243)
                     /site_type="other"
                     /note="flanking leucine-zipper layers"
                     /db_xref="CDD:277192"
     Site            236
                     /site_type="active"
                     /note="zero layer [active]"
                     /db_xref="CDD:277192"
     CDS             1..296
                     /gene="LOC117609533"
                     /coded_by="XM_034336008.2:656..1546"
                     /db_xref="GeneID:117609533"
ORIGIN      
        1 mvrdrmpelr acqnnsttfg kgflqdvhiq isqnkklkev lneveevral ievitenisi
       61 ikdlhnnvla ytnkdmqkel enrtytisqt sfriqrklre mgkeiasidd ltlssandgp
      121 ihirikalqy ttmlrlfsei meeynismlr yhekcrlllq qqkilirkri tseeleklld
      181 nqgnslfvdn iledsriarq qlsdiqirhn diikieksit evrdmftema fliekqgdql
      241 nsveyfagkt adnvdsgrtd lkkaeqhshr hrkrkiklci iisiiiivfl liiifv
//
LOCUS       XP_034191911             303 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609543 [Osmia lignaria lignaria].
ACCESSION   XP_034191911
VERSION     XP_034191911.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="uncharacterized protein LOC117609543"
                     /calculated_mol_wt=31853
     Region          13..231
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          13..40
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          42..73
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          75..106
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(108,110,114..115,118..120,122..123,127,130,139,141,
                     143,147..148,151..153,155..156,160,163,172,174,176,
                     180..181,184..186,188..189,193,196)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          108..139
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          141..172
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          174..203
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          207..236
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          212..295
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          239..270
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..303
                     /gene="LOC117609543"
                     /coded_by="XM_034336020.2:326..1237"
                     /db_xref="GeneID:117609543"
ORIGIN      
        1 msmkkesprd velhlaaahg dtkrlrilld sgrvhvdckd kdgttplila aagghieavn
       61 ellqqgadpn arrltgttal ffaaqggymd ianlllkhda vvdscsidgg tplfvacqcg
      121 hldvveglie rganpnahmk dgatalfiaa qnghvrilev llehgaktda artdgatplw
      181 igaqmghdhv vrrllkagak vdatrhdgat plfkaahkgh aavvgellky rpslgilpng
      241 esalhaaalt gqmtvarqlv gagadpllvn qegvtplqla irhsqtqvan ylkdklrtrt
      301 gas
//
LOCUS       XP_034191925             271 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase PGAM5, mitochondrial isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191925
VERSION     XP_034191925.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..271
                     /product="serine/threonine-protein phosphatase PGAM5,
                     mitochondrial isoform X1"
                     /calculated_mol_wt=31358
     Region          <31..271
                     /region_name="HP"
                     /note="Histidine phosphatase domain found in a
                     functionally diverse set of proteins, mostly phosphatases;
                     contains a His residue which is phosphorylated during the
                     reaction; cl11399"
                     /db_xref="CDD:472174"
     Site            order(87..88,135,212..213)
                     /site_type="active"
                     /note="catalytic core [active]"
                     /db_xref="CDD:132718"
     CDS             1..271
                     /gene="LOC117609547"
                     /coded_by="XM_034336034.2:423..1238"
                     /db_xref="GeneID:117609547"
ORIGIN      
        1 mppssnfkkw itgfgaigga mlfypndnhn switpplwqn kwdhnwdrrh sewskmtkrd
       61 vesdkqiyge tlvkknadik yniilvrhgq yniegktdld rtlttlgrqq aeatgkrlqe
      121 lklpydlivq stivraketa qiiknylrdv tvkedsglte gmpippdppi nfwksdvnfy
      181 edgprieaaf rkyfhrpdpn qkkdsyiilv chanviryfv cralqfppqg wlrlslnhas
      241 itwisirpng rvtlrnlgns ghmkpyliss h
//
LOCUS       XP_034191943             265 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein phosphatase PGAM5, mitochondrial isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191943
VERSION     XP_034191943.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336052.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..265
                     /product="serine/threonine-protein phosphatase PGAM5,
                     mitochondrial isoform X2"
                     /calculated_mol_wt=30677
     Region          <31..258
                     /region_name="HP"
                     /note="Histidine phosphatase domain found in a
                     functionally diverse set of proteins, mostly phosphatases;
                     contains a His residue which is phosphorylated during the
                     reaction; cl11399"
                     /db_xref="CDD:472174"
     Site            order(87..88,135,212..213)
                     /site_type="active"
                     /note="catalytic core [active]"
                     /db_xref="CDD:132718"
     CDS             1..265
                     /gene="LOC117609547"
                     /coded_by="XM_034336052.2:423..1220"
                     /db_xref="GeneID:117609547"
ORIGIN      
        1 mppssnfkkw itgfgaigga mlfypndnhn switpplwqn kwdhnwdrrh sewskmtkrd
       61 vesdkqiyge tlvkknadik yniilvrhgq yniegktdld rtlttlgrqq aeatgkrlqe
      121 lklpydlivq stivraketa qiiknylrdv tvkedsglte gmpippdppi nfwksdvnfy
      181 edgprieaaf rkyfhrpdpn qkkdsyiilv chanviryfv cralqfppqg wlrlslnhas
      241 itwisirpng rvtlrnlgnq svnnd
//
LOCUS       XP_034192557            2091 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192557
VERSION     XP_034192557.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336666.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2091
                     /product="otoferlin isoform X1"
                     /calculated_mol_wt=237554
     Region          14..129
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          309..419
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          417..466
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          474..623
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(503,509,557,559,570,572)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          888..963
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1007..1144
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1023,1029,1085,1087)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1567..1712
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1582,1588,1659,1661,1666)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1828..1960
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1970..2088
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2091
                     /gene="LOC117609906"
                     /coded_by="XM_034336666.2:388..6663"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 mlprytvnaf eypsitflia rditrrrtik arlnlglsyp sylpkfqfll qsfiwnlgrp
       61 vdeaevlqlt vvsrgvlrne kvlakyglvl qtvaregriv itdslvdlnn kplpavvcfe
      121 irynppdgsc spseimepse imedeqqmli dieqnianle rcldqansgs gsgkrkgswh
      181 speknsrrgf lqrgssmsaa eksperksrs stlksmrsfm klgkqrpprt rscdsgsdtr
      241 elldrrdssc atsnepsran smtslesnas dydsqvsanp sepqeaiarp akkpkpkttd
      301 tgqtalkaqd yqvcitiiea rqlaglnmdp vvcvqigdqr kytsvkestn cpyyneyfvf
      361 dfhmppvmlf dkiitlsvqq srnllranlt lgifkldiat vwaqpdhqfy hkwalltdpd
      421 dvaggpkgyl kcdisvigkg dtvkippkse kdeddiegnl llpdgvpier qrakfivkvy
      481 radglpkmns simanvkkaf tgeikdlvdp yvqvsfaglt gktsvkrhsy apvwneqivf
      541 semfpplcqr ikiqlcdndp vhatvigthf vdlkqisndg ekgflptfgp afihfygsir
      601 dyslidqhst lnvglgegis yrarllvair teisdnieia qseveveptv pinelayarn
      661 eefflfatim datmidkklg dkpmyfelsi gnagnaldgh nesskmcelg pksgtsgdqe
      721 elqevlsgsw qsttpaskpm thdkiyyflp ywddkpclhv rsiwpdyrrr mynsniigki
      781 adkleeglse tqlhvddcsg ekilkstlee lssncnryvs iskssltgpg vgktkldker
      841 tklcqrelen igimsrnlka vvtkssfker lktaqgylqk lkflledpqd slpdvfvwvi
      901 ssgrrvayqr ipgrdliysi vdeecgkscg kvqtmflklp gkkrfgpsgw aiqtklqiym
      961 wmgilkhkky fiqglpkgye ishevknver pralpptiih yvekhkfelr ahmyqarsli
     1021 gsdasglsdp farvicgefc kstqvidetl sptwdelllf ddiliygtae eikkdppsiv
     1081 ieifdqdkvg kseyigrava rphvklases ytppefppsl ewydvsrgaa ragellavfe
     1141 lleypstkdy gfptlpdpke rlpqtttsll dqgpifpvpv girptlskyr ievlfwglrd
     1201 lkrihlmtvd kprvdvecag hilyssvian akknpnfntp ikflelelpe qelyrpplti
     1261 ravdcrsfgr ytlvgthtin sihkymycpq tkkakdaedr kknlyqlqqy tagfdtsktk
     1321 yhqsslpesl tdvefnngdt iitlglelaw ptkrdkteqn irkkqslvnd qstgdfsnfd
     1381 dedgcqdwwt kyfasveami eenkelrkek pmfpaqsngt vpmyleenys qaqmnlsger
     1441 spgvatkklf glkstanaar fvskispkqt yqkfpktall kiypneleaq pqfeqfkewl
     1501 htfelyrgkk tgdepedesr ivgsfkgalk vykwplprdl idhtvmgfdp qygffqgvps
     1561 nepihvlvrv yivkandlhp cdlngkadpy vvlqlggkri sdkehyvskq lnpvfgkyvt
     1621 idlsipeyvi slscrlfprc feieatfpqd slltvqvldw dlvgaddmig etkidlenrf
     1681 ysrhratcgl akkydesgyn kwrdamkptq ilsklckdgk vdgpvyshgk vtigrktfsl
     1741 aneemeyyvh akgieeqlal avlhqwhhfp rfgcalvpeh vetrplynpe kpgieqgkle
     1801 mwvdmfpmdm pspsasidis prkpksyelr iiiwntddvv leddafftge kmsdiyvkgw
     1861 lkgpedcqst dihyrsltge gnfnwrfifp fdylvaeeki vinrkeslfs wdeteckipa
     1921 rlelqvwdad hfsaddflga itldlnrfpr gaknsklctl smlktdgtvp tvnifkqkri
     1981 kgwwpfyvkk enedmeltgk veaeihlltk eeaeknpagy grnepdpldk pkrpdasfmw
     2041 flnplksiky ivwhnykwai lkafvtigli alvllffyai pgysvkkllg a
//
LOCUS       XP_034192560            2090 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192560
VERSION     XP_034192560.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336669.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2090
                     /product="otoferlin isoform X2"
                     /calculated_mol_wt=237455
     Region          14..129
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          309..419
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          417..466
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          474..623
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(503,509,557,559,570,572)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          888..963
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1007..1143
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1023,1029,1085,1087)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1566..1711
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1581,1587,1658,1660,1665)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1827..1959
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1969..2087
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2090
                     /gene="LOC117609906"
                     /coded_by="XM_034336669.2:388..6660"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 mlprytvnaf eypsitflia rditrrrtik arlnlglsyp sylpkfqfll qsfiwnlgrp
       61 vdeaevlqlt vvsrgvlrne kvlakyglvl qtvaregriv itdslvdlnn kplpavvcfe
      121 irynppdgsc spseimepse imedeqqmli dieqnianle rcldqansgs gsgkrkgswh
      181 speknsrrgf lqrgssmsaa eksperksrs stlksmrsfm klgkqrpprt rscdsgsdtr
      241 elldrrdssc atsnepsran smtslesnas dydsqvsanp sepqeaiarp akkpkpkttd
      301 tgqtalkaqd yqvcitiiea rqlaglnmdp vvcvqigdqr kytsvkestn cpyyneyfvf
      361 dfhmppvmlf dkiitlsvqq srnllranlt lgifkldiat vwaqpdhqfy hkwalltdpd
      421 dvaggpkgyl kcdisvigkg dtvkippkse kdeddiegnl llpdgvpier qrakfivkvy
      481 radglpkmns simanvkkaf tgeikdlvdp yvqvsfaglt gktsvkrhsy apvwneqivf
      541 semfpplcqr ikiqlcdndp vhatvigthf vdlkqisndg ekgflptfgp afihfygsir
      601 dyslidqhst lnvglgegis yrarllvair teisdnieia qseveveptv pinelayarn
      661 eefflfatim datmidkklg dkpmyfelsi gnagnaldgh nesskmcelg pksgtsgdqe
      721 elqevlsgsw qsttpaskpm thdkiyyflp ywddkpclhv rsiwpdyrrr mynsniigki
      781 adkleeglse tqlhvddcsg ekilkstlee lssncnryvs iskssltgpg vgktkldker
      841 tklcqrelen igimsrnlka vvtkssfker lktaqgylqk lkflledpqd slpdvfvwvi
      901 ssgrrvayqr ipgrdliysi vdeecgkscg kvqtmflklp gkkrfgpsgw aiqtklqiym
      961 wmgilkhkky fiqglpkgye ishevknver pralpptiih yvekhkfelr ahmyqarsli
     1021 gsdasglsdp farvicgefc kstqvidetl sptwdelllf ddiliygtae eikkdppsiv
     1081 ieifdqdkgk seyigravar phvklasesy tppefppsle wydvsrgaar agellavfel
     1141 leypstkdyg fptlpdpker lpqtttslld qgpifpvpvg irptlskyri evlfwglrdl
     1201 krihlmtvdk prvdvecagh ilyssviana kknpnfntpi kflelelpeq elyrppltir
     1261 avdcrsfgry tlvgthtins ihkymycpqt kkakdaedrk knlyqlqqyt agfdtsktky
     1321 hqsslpeslt dvefnngdti itlglelawp tkrdkteqni rkkqslvndq stgdfsnfdd
     1381 edgcqdwwtk yfasveamie enkelrkekp mfpaqsngtv pmyleenysq aqmnlsgers
     1441 pgvatkklfg lkstanaarf vskispkqty qkfpktallk iypneleaqp qfeqfkewlh
     1501 tfelyrgkkt gdepedesri vgsfkgalkv ykwplprdli dhtvmgfdpq ygffqgvpsn
     1561 epihvlvrvy ivkandlhpc dlngkadpyv vlqlggkris dkehyvskql npvfgkyvti
     1621 dlsipeyvis lscrlfprcf eieatfpqds lltvqvldwd lvgaddmige tkidlenrfy
     1681 srhratcgla kkydesgynk wrdamkptqi lsklckdgkv dgpvyshgkv tigrktfsla
     1741 neemeyyvha kgieeqlala vlhqwhhfpr fgcalvpehv etrplynpek pgieqgklem
     1801 wvdmfpmdmp spsasidisp rkpksyelri iiwntddvvl eddafftgek msdiyvkgwl
     1861 kgpedcqstd ihyrsltgeg nfnwrfifpf dylvaeekiv inrkeslfsw deteckipar
     1921 lelqvwdadh fsaddflgai tldlnrfprg aknsklctls mlktdgtvpt vnifkqkrik
     1981 gwwpfyvkke nedmeltgkv eaeihlltke eaeknpagyg rnepdpldkp krpdasfmwf
     2041 lnplksikyi vwhnykwail kafvtiglia lvllffyaip gysvkkllga
//
LOCUS       XP_034192567            2090 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034192567
VERSION     XP_034192567.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336676.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2090
                     /product="otoferlin isoform X3"
                     /calculated_mol_wt=237467
     Region          14..129
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          308..418
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          416..465
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          473..622
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(502,508,556,558,569,571)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          887..962
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1006..1143
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1022,1028,1084,1086)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1566..1711
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1581,1587,1658,1660,1665)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1827..1959
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1969..2087
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2090
                     /gene="LOC117609906"
                     /coded_by="XM_034336676.2:388..6660"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 mlprytvnaf eypsitflia rditrrrtik arlnlglsyp sylpkfqfll qsfiwnlgrp
       61 vdeaevlqlt vvsrgvlrne kvlakyglvl qtvaregriv itdslvdlnn kplpavvcfe
      121 irynppdgsc spseimepse imedeqqmli dieqnianle rcldqansgs gsgkrkgswh
      181 speknsrrgf lqrgssmsaa eksperkrss tlksmrsfmk lgkqrpprtr scdsgsdtre
      241 lldrrdssca tsnepsrans mtslesnasd ydsqvsanps epqeaiarpa kkpkpkttdt
      301 gqtalkaqdy qvcitiiear qlaglnmdpv vcvqigdqrk ytsvkestnc pyyneyfvfd
      361 fhmppvmlfd kiitlsvqqs rnllranltl gifkldiatv waqpdhqfyh kwalltdpdd
      421 vaggpkgylk cdisvigkgd tvkippksek deddiegnll lpdgvpierq rakfivkvyr
      481 adglpkmnss imanvkkaft geikdlvdpy vqvsfagltg ktsvkrhsya pvwneqivfs
      541 emfpplcqri kiqlcdndpv hatvigthfv dlkqisndge kgflptfgpa fihfygsird
      601 yslidqhstl nvglgegisy rarllvairt eisdnieiaq seveveptvp inelayarne
      661 efflfatimd atmidkklgd kpmyfelsig nagnaldghn esskmcelgp ksgtsgdqee
      721 lqevlsgswq sttpaskpmt hdkiyyflpy wddkpclhvr siwpdyrrrm ynsniigkia
      781 dkleeglset qlhvddcsge kilkstleel ssncnryvsi skssltgpgv gktkldkert
      841 klcqreleni gimsrnlkav vtkssfkerl ktaqgylqkl kflledpqds lpdvfvwvis
      901 sgrrvayqri pgrdliysiv deecgkscgk vqtmflklpg kkrfgpsgwa iqtklqiymw
      961 mgilkhkkyf iqglpkgyei shevknverp ralpptiihy vekhkfelra hmyqarslig
     1021 sdasglsdpf arvicgefck stqvidetls ptwdelllfd diliygtaee ikkdppsivi
     1081 eifdqdkvgk seyigravar phvklasesy tppefppsle wydvsrgaar agellavfel
     1141 leypstkdyg fptlpdpker lpqtttslld qgpifpvpvg irptlskyri evlfwglrdl
     1201 krihlmtvdk prvdvecagh ilyssviana kknpnfntpi kflelelpeq elyrppltir
     1261 avdcrsfgry tlvgthtins ihkymycpqt kkakdaedrk knlyqlqqyt agfdtsktky
     1321 hqsslpeslt dvefnngdti itlglelawp tkrdkteqni rkkqslvndq stgdfsnfdd
     1381 edgcqdwwtk yfasveamie enkelrkekp mfpaqsngtv pmyleenysq aqmnlsgers
     1441 pgvatkklfg lkstanaarf vskispkqty qkfpktallk iypneleaqp qfeqfkewlh
     1501 tfelyrgkkt gdepedesri vgsfkgalkv ykwplprdli dhtvmgfdpq ygffqgvpsn
     1561 epihvlvrvy ivkandlhpc dlngkadpyv vlqlggkris dkehyvskql npvfgkyvti
     1621 dlsipeyvis lscrlfprcf eieatfpqds lltvqvldwd lvgaddmige tkidlenrfy
     1681 srhratcgla kkydesgynk wrdamkptqi lsklckdgkv dgpvyshgkv tigrktfsla
     1741 neemeyyvha kgieeqlala vlhqwhhfpr fgcalvpehv etrplynpek pgieqgklem
     1801 wvdmfpmdmp spsasidisp rkpksyelri iiwntddvvl eddafftgek msdiyvkgwl
     1861 kgpedcqstd ihyrsltgeg nfnwrfifpf dylvaeekiv inrkeslfsw deteckipar
     1921 lelqvwdadh fsaddflgai tldlnrfprg aknsklctls mlktdgtvpt vnifkqkrik
     1981 gwwpfyvkke nedmeltgkv eaeihlltke eaeknpagyg rnepdpldkp krpdasfmwf
     2041 lnplksikyi vwhnykwail kafvtiglia lvllffyaip gysvkkllga
//
LOCUS       XP_034192569            2086 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034192569
VERSION     XP_034192569.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336678.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2086
                     /product="otoferlin isoform X4"
                     /calculated_mol_wt=236644
     Region          3..124
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          304..414
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          412..461
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          469..618
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(498,504,552,554,565,567)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          883..958
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1002..1139
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1018,1024,1080,1082)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1562..1707
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1577,1583,1654,1656,1661)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1823..1955
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1965..2083
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2086
                     /gene="LOC117609906"
                     /coded_by="XM_034336678.2:423..6683"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 malvvtvknf qglkhrgdkv vkvdfrgvsh yskcleengd hiaveesfiw nlgrpvdeae
       61 vlqltvvsrg vlrnekvlak yglvlqtvar egrivitdsl vdlnnkplpa vvcfeirynp
      121 pdgscspsei mepseimede qqmlidieqn ianlercldq ansgsgsgkr kgswhspekn
      181 srrgflqrgs smsaaekspe rksrsstlks mrsfmklgkq rpprtrscds gsdtrelldr
      241 rdsscatsne psransmtsl esnasdydsq vsanpsepqe aiarpakkpk pkttdtgqta
      301 lkaqdyqvci tiiearqlag lnmdpvvcvq igdqrkytsv kestncpyyn eyfvfdfhmp
      361 pvmlfdkiit lsvqqsrnll ranltlgifk ldiatvwaqp dhqfyhkwal ltdpddvagg
      421 pkgylkcdis vigkgdtvki ppksekdedd iegnlllpdg vpierqrakf ivkvyradgl
      481 pkmnssiman vkkaftgeik dlvdpyvqvs fagltgktsv krhsyapvwn eqivfsemfp
      541 plcqrikiql cdndpvhatv igthfvdlkq isndgekgfl ptfgpafihf ygsirdysli
      601 dqhstlnvgl gegisyrarl lvairteisd nieiaqseve veptvpinel ayarneeffl
      661 fatimdatmi dkklgdkpmy felsignagn aldghnessk mcelgpksgt sgdqeelqev
      721 lsgswqsttp askpmthdki yyflpywddk pclhvrsiwp dyrrrmynsn iigkiadkle
      781 eglsetqlhv ddcsgekilk stleelssnc nryvsiskss ltgpgvgktk ldkertklcq
      841 relenigims rnlkavvtks sfkerlktaq gylqklkfll edpqdslpdv fvwvissgrr
      901 vayqripgrd liysivdeec gkscgkvqtm flklpgkkrf gpsgwaiqtk lqiymwmgil
      961 khkkyfiqgl pkgyeishev knverpralp ptiihyvekh kfelrahmyq arsligsdas
     1021 glsdpfarvi cgefckstqv idetlsptwd elllfddili ygtaeeikkd ppsivieifd
     1081 qdkvgkseyi gravarphvk lasesytppe fppslewydv srgaaragel lavfelleyp
     1141 stkdygfptl pdpkerlpqt ttslldqgpi fpvpvgirpt lskyrievlf wglrdlkrih
     1201 lmtvdkprvd vecaghilys svianakknp nfntpikfle lelpeqelyr ppltiravdc
     1261 rsfgrytlvg thtinsihky mycpqtkkak daedrkknly qlqqytagfd tsktkyhqss
     1321 lpesltdvef nngdtiitlg lelawptkrd kteqnirkkq slvndqstgd fsnfddedgc
     1381 qdwwtkyfas veamieenke lrkekpmfpa qsngtvpmyl eenysqaqmn lsgerspgva
     1441 tkklfglkst anaarfvski spkqtyqkfp ktallkiypn eleaqpqfeq fkewlhtfel
     1501 yrgkktgdep edesrivgsf kgalkvykwp lprdlidhtv mgfdpqygff qgvpsnepih
     1561 vlvrvyivka ndlhpcdlng kadpyvvlql ggkrisdkeh yvskqlnpvf gkyvtidlsi
     1621 peyvislscr lfprcfeiea tfpqdslltv qvldwdlvga ddmigetkid lenrfysrhr
     1681 atcglakkyd esgynkwrda mkptqilskl ckdgkvdgpv yshgkvtigr ktfslaneem
     1741 eyyvhakgie eqlalavlhq whhfprfgca lvpehvetrp lynpekpgie qgklemwvdm
     1801 fpmdmpspsa sidisprkpk syelriiiwn tddvvledda fftgekmsdi yvkgwlkgpe
     1861 dcqstdihyr sltgegnfnw rfifpfdylv aeekivinrk eslfswdete ckiparlelq
     1921 vwdadhfsad dflgaitldl nrfprgakns klctlsmlkt dgtvptvnif kqkrikgwwp
     1981 fyvkkenedm eltgkveaei hlltkeeaek npagygrnep dpldkpkrpd asfmwflnpl
     2041 ksikyivwhn ykwailkafv tiglialvll ffyaipgysv kkllga
//
LOCUS       XP_034192588            2069 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034192588
VERSION     XP_034192588.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336697.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2069
                     /product="otoferlin isoform X5"
                     /calculated_mol_wt=234987
     Region          14..129
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          309..419
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          417..466
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          474..623
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(503,509,557,559,570,572)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          888..963
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1007..1144
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1023,1029,1085,1087)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1567..1690
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1582,1588,1637,1639,1644)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1806..1938
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1948..2066
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2069
                     /gene="LOC117609906"
                     /coded_by="XM_034336697.2:388..6597"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 mlprytvnaf eypsitflia rditrrrtik arlnlglsyp sylpkfqfll qsfiwnlgrp
       61 vdeaevlqlt vvsrgvlrne kvlakyglvl qtvaregriv itdslvdlnn kplpavvcfe
      121 irynppdgsc spseimepse imedeqqmli dieqnianle rcldqansgs gsgkrkgswh
      181 speknsrrgf lqrgssmsaa eksperksrs stlksmrsfm klgkqrpprt rscdsgsdtr
      241 elldrrdssc atsnepsran smtslesnas dydsqvsanp sepqeaiarp akkpkpkttd
      301 tgqtalkaqd yqvcitiiea rqlaglnmdp vvcvqigdqr kytsvkestn cpyyneyfvf
      361 dfhmppvmlf dkiitlsvqq srnllranlt lgifkldiat vwaqpdhqfy hkwalltdpd
      421 dvaggpkgyl kcdisvigkg dtvkippkse kdeddiegnl llpdgvpier qrakfivkvy
      481 radglpkmns simanvkkaf tgeikdlvdp yvqvsfaglt gktsvkrhsy apvwneqivf
      541 semfpplcqr ikiqlcdndp vhatvigthf vdlkqisndg ekgflptfgp afihfygsir
      601 dyslidqhst lnvglgegis yrarllvair teisdnieia qseveveptv pinelayarn
      661 eefflfatim datmidkklg dkpmyfelsi gnagnaldgh nesskmcelg pksgtsgdqe
      721 elqevlsgsw qsttpaskpm thdkiyyflp ywddkpclhv rsiwpdyrrr mynsniigki
      781 adkleeglse tqlhvddcsg ekilkstlee lssncnryvs iskssltgpg vgktkldker
      841 tklcqrelen igimsrnlka vvtkssfker lktaqgylqk lkflledpqd slpdvfvwvi
      901 ssgrrvayqr ipgrdliysi vdeecgkscg kvqtmflklp gkkrfgpsgw aiqtklqiym
      961 wmgilkhkky fiqglpkgye ishevknver pralpptiih yvekhkfelr ahmyqarsli
     1021 gsdasglsdp farvicgefc kstqvidetl sptwdelllf ddiliygtae eikkdppsiv
     1081 ieifdqdkvg kseyigrava rphvklases ytppefppsl ewydvsrgaa ragellavfe
     1141 lleypstkdy gfptlpdpke rlpqtttsll dqgpifpvpv girptlskyr ievlfwglrd
     1201 lkrihlmtvd kprvdvecag hilyssvian akknpnfntp ikflelelpe qelyrpplti
     1261 ravdcrsfgr ytlvgthtin sihkymycpq tkkakdaedr kknlyqlqqy tagfdtsktk
     1321 yhqsslpesl tdvefnngdt iitlglelaw ptkrdkteqn irkkqslvnd qstgdfsnfd
     1381 dedgcqdwwt kyfasveami eenkelrkek pmfpaqsngt vpmyleenys qaqmnlsger
     1441 spgvatkklf glkstanaar fvskispkqt yqkfpktall kiypneleaq pqfeqfkewl
     1501 htfelyrgkk tgdepedesr ivgsfkgalk vykwplprdl idhtvmgfdp qygffqgvps
     1561 nepihvlvrv yivkandlhp cdlngkadpy vvlqlggkri sdkehyvskq lnpvfgkcfe
     1621 ieatfpqdsl ltvqvldwdl vgaddmiget kidlenrfys rhratcglak kydesgynkw
     1681 rdamkptqil sklckdgkvd gpvyshgkvt igrktfslan eemeyyvhak gieeqlalav
     1741 lhqwhhfprf gcalvpehve trplynpekp gieqgklemw vdmfpmdmps psasidispr
     1801 kpksyelrii iwntddvvle ddafftgekm sdiyvkgwlk gpedcqstdi hyrsltgegn
     1861 fnwrfifpfd ylvaeekivi nrkeslfswd eteckiparl elqvwdadhf saddflgait
     1921 ldlnrfprga knsklctlsm lktdgtvptv nifkqkrikg wwpfyvkken edmeltgkve
     1981 aeihlltkee aeknpagygr nepdpldkpk rpdasfmwfl nplksikyiv whnykwailk
     2041 afvtiglial vllffyaipg ysvkkllga
//
LOCUS       XP_034192596            2064 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034192596
VERSION     XP_034192596.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336705.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2064
                     /product="otoferlin isoform X6"
                     /calculated_mol_wt=234077
     Region          3..124
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          304..414
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          412..461
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          469..618
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(498,504,552,554,565,567)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          883..958
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1002..1139
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1018,1024,1080,1082)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1562..1685
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1577,1583,1632,1634,1639)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1801..1933
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1943..2061
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2064
                     /gene="LOC117609906"
                     /coded_by="XM_034336705.2:423..6617"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 malvvtvknf qglkhrgdkv vkvdfrgvsh yskcleengd hiaveesfiw nlgrpvdeae
       61 vlqltvvsrg vlrnekvlak yglvlqtvar egrivitdsl vdlnnkplpa vvcfeirynp
      121 pdgscspsei mepseimede qqmlidieqn ianlercldq ansgsgsgkr kgswhspekn
      181 srrgflqrgs smsaaekspe rksrsstlks mrsfmklgkq rpprtrscds gsdtrelldr
      241 rdsscatsne psransmtsl esnasdydsq vsanpsepqe aiarpakkpk pkttdtgqta
      301 lkaqdyqvci tiiearqlag lnmdpvvcvq igdqrkytsv kestncpyyn eyfvfdfhmp
      361 pvmlfdkiit lsvqqsrnll ranltlgifk ldiatvwaqp dhqfyhkwal ltdpddvagg
      421 pkgylkcdis vigkgdtvki ppksekdedd iegnlllpdg vpierqrakf ivkvyradgl
      481 pkmnssiman vkkaftgeik dlvdpyvqvs fagltgktsv krhsyapvwn eqivfsemfp
      541 plcqrikiql cdndpvhatv igthfvdlkq isndgekgfl ptfgpafihf ygsirdysli
      601 dqhstlnvgl gegisyrarl lvairteisd nieiaqseve veptvpinel ayarneeffl
      661 fatimdatmi dkklgdkpmy felsignagn aldghnessk mcelgpksgt sgdqeelqev
      721 lsgswqsttp askpmthdki yyflpywddk pclhvrsiwp dyrrrmynsn iigkiadkle
      781 eglsetqlhv ddcsgekilk stleelssnc nryvsiskss ltgpgvgktk ldkertklcq
      841 relenigims rnlkavvtks sfkerlktaq gylqklkfll edpqdslpdv fvwvissgrr
      901 vayqripgrd liysivdeec gkscgkvqtm flklpgkkrf gpsgwaiqtk lqiymwmgil
      961 khkkyfiqgl pkgyeishev knverpralp ptiihyvekh kfelrahmyq arsligsdas
     1021 glsdpfarvi cgefckstqv idetlsptwd elllfddili ygtaeeikkd ppsivieifd
     1081 qdkvgkseyi gravarphvk lasesytppe fppslewydv srgaaragel lavfelleyp
     1141 stkdygfptl pdpkerlpqt ttslldqgpi fpvpvgirpt lskyrievlf wglrdlkrih
     1201 lmtvdkprvd vecaghilys svianakknp nfntpikfle lelpeqelyr ppltiravdc
     1261 rsfgrytlvg thtinsihky mycpqtkkak daedrkknly qlqqytagfd tsktkyhqss
     1321 lpesltdvef nngdtiitlg lelawptkrd kteqnirkkq slvndqstgd fsnfddedgc
     1381 qdwwtkyfas veamieenke lrkekpmfpa qsngtvpmyl eenysqaqmn lsgerspgva
     1441 tkklfglkst anaarfvski spkqtyqkfp ktallkiypn eleaqpqfeq fkewlhtfel
     1501 yrgkktgdep edesrivgsf kgalkvykwp lprdlidhtv mgfdpqygff qgvpsnepih
     1561 vlvrvyivka ndlhpcdlng kadpyvvlql ggkrisdkeh yvskqlnpvf gkcfeieatf
     1621 pqdslltvqv ldwdlvgadd migetkidle nrfysrhrat cglakkydes gynkwrdamk
     1681 ptqilsklck dgkvdgpvys hgkvtigrkt fslaneemey yvhakgieeq lalavlhqwh
     1741 hfprfgcalv pehvetrply npekpgieqg klemwvdmfp mdmpspsasi disprkpksy
     1801 elriiiwntd dvvleddaff tgekmsdiyv kgwlkgpedc qstdihyrsl tgegnfnwrf
     1861 ifpfdylvae ekivinrkes lfswdeteck iparlelqvw dadhfsaddf lgaitldlnr
     1921 fprgaknskl ctlsmlktdg tvptvnifkq krikgwwpfy vkkenedmel tgkveaeihl
     1981 ltkeeaeknp agygrnepdp ldkpkrpdas fmwflnplks ikyivwhnyk wailkafvti
     2041 glialvllff yaipgysvkk llga
//
LOCUS       XP_034192605            2063 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_034192605
VERSION     XP_034192605.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336714.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2063
                     /product="otoferlin isoform X7"
                     /calculated_mol_wt=233990
     Region          3..124
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          303..413
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          411..460
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          468..617
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(497,503,551,553,564,566)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          882..957
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1001..1138
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1017,1023,1079,1081)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1561..1684
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1576,1582,1631,1633,1638)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1800..1932
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1942..2060
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2063
                     /gene="LOC117609906"
                     /coded_by="XM_034336714.2:423..6614"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 malvvtvknf qglkhrgdkv vkvdfrgvsh yskcleengd hiaveesfiw nlgrpvdeae
       61 vlqltvvsrg vlrnekvlak yglvlqtvar egrivitdsl vdlnnkplpa vvcfeirynp
      121 pdgscspsei mepseimede qqmlidieqn ianlercldq ansgsgsgkr kgswhspekn
      181 srrgflqrgs smsaaekspe rkrsstlksm rsfmklgkqr pprtrscdsg sdtrelldrr
      241 dsscatsnep sransmtsle snasdydsqv sanpsepqea iarpakkpkp kttdtgqtal
      301 kaqdyqvcit iiearqlagl nmdpvvcvqi gdqrkytsvk estncpyyne yfvfdfhmpp
      361 vmlfdkiitl svqqsrnllr anltlgifkl diatvwaqpd hqfyhkwall tdpddvaggp
      421 kgylkcdisv igkgdtvkip pksekdeddi egnlllpdgv pierqrakfi vkvyradglp
      481 kmnssimanv kkaftgeikd lvdpyvqvsf agltgktsvk rhsyapvwne qivfsemfpp
      541 lcqrikiqlc dndpvhatvi gthfvdlkqi sndgekgflp tfgpafihfy gsirdyslid
      601 qhstlnvglg egisyrarll vairteisdn ieiaqsevev eptvpinela yarneefflf
      661 atimdatmid kklgdkpmyf elsignagna ldghnesskm celgpksgts gdqeelqevl
      721 sgswqsttpa skpmthdkiy yflpywddkp clhvrsiwpd yrrrmynsni igkiadklee
      781 glsetqlhvd dcsgekilks tleelssncn ryvsiskssl tgpgvgktkl dkertklcqr
      841 elenigimsr nlkavvtkss fkerlktaqg ylqklkflle dpqdslpdvf vwvissgrrv
      901 ayqripgrdl iysivdeecg kscgkvqtmf lklpgkkrfg psgwaiqtkl qiymwmgilk
      961 hkkyfiqglp kgyeishevk nverpralpp tiihyvekhk felrahmyqa rsligsdasg
     1021 lsdpfarvic gefckstqvi detlsptwde lllfddiliy gtaeeikkdp psivieifdq
     1081 dkvgkseyig ravarphvkl asesytppef ppslewydvs rgaaragell avfelleyps
     1141 tkdygfptlp dpkerlpqtt tslldqgpif pvpvgirptl skyrievlfw glrdlkrihl
     1201 mtvdkprvdv ecaghilyss vianakknpn fntpikflel elpeqelyrp pltiravdcr
     1261 sfgrytlvgt htinsihkym ycpqtkkakd aedrkknlyq lqqytagfdt sktkyhqssl
     1321 pesltdvefn ngdtiitlgl elawptkrdk teqnirkkqs lvndqstgdf snfddedgcq
     1381 dwwtkyfasv eamieenkel rkekpmfpaq sngtvpmyle enysqaqmnl sgerspgvat
     1441 kklfglksta naarfvskis pkqtyqkfpk tallkiypne leaqpqfeqf kewlhtfely
     1501 rgkktgdepe desrivgsfk galkvykwpl prdlidhtvm gfdpqygffq gvpsnepihv
     1561 lvrvyivkan dlhpcdlngk adpyvvlqlg gkrisdkehy vskqlnpvfg kcfeieatfp
     1621 qdslltvqvl dwdlvgaddm igetkidlen rfysrhratc glakkydesg ynkwrdamkp
     1681 tqilsklckd gkvdgpvysh gkvtigrktf slaneemeyy vhakgieeql alavlhqwhh
     1741 fprfgcalvp ehvetrplyn pekpgieqgk lemwvdmfpm dmpspsasid isprkpksye
     1801 lriiiwntdd vvleddafft gekmsdiyvk gwlkgpedcq stdihyrslt gegnfnwrfi
     1861 fpfdylvaee kivinrkesl fswdetecki parlelqvwd adhfsaddfl gaitldlnrf
     1921 prgaknsklc tlsmlktdgt vptvnifkqk rikgwwpfyv kkenedmelt gkveaeihll
     1981 tkeeaeknpa gygrnepdpl dkpkrpdasf mwflnplksi kyivwhnykw ailkafvtig
     2041 lialvllffy aipgysvkkl lga
//
LOCUS       XP_034192614            2046 aa            linear   INV 03-SEP-2025
DEFINITION  otoferlin isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034192614
VERSION     XP_034192614.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336723.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2046
                     /product="otoferlin isoform X8"
                     /calculated_mol_wt=232476
     Region          14..129
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          309..419
                     /region_name="C2B_Ferlin"
                     /note="C2 domain second repeat in Ferlin; cd04011"
                     /db_xref="CDD:175978"
     Region          417..466
                     /region_name="FerI"
                     /note="FerI (NUC094) domain; pfam08151"
                     /db_xref="CDD:462377"
     Region          474..623
                     /region_name="C2C_Ferlin"
                     /note="C2 domain third repeat in Ferlin; cd04018"
                     /db_xref="CDD:175985"
     Site            order(503,509,557,559,570,572)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175985"
     Region          888..963
                     /region_name="FerB"
                     /note="FerB (NUC096) domain; pfam08150"
                     /db_xref="CDD:462376"
     Region          1007..1144
                     /region_name="C2D_Ferlin"
                     /note="C2 domain fourth repeat in Ferlin; cd04017"
                     /db_xref="CDD:175984"
     Site            order(1023,1029,1085,1087)
                     /site_type="other"
                     /note="putative Ca2+ binding site [ion binding]"
                     /db_xref="CDD:175984"
     Region          1522..1667
                     /region_name="C2E_Ferlin"
                     /note="C2 domain fifth repeat in Ferlin; cd04037"
                     /db_xref="CDD:176002"
     Site            order(1537,1543,1614,1616,1621)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:176002"
     Region          1783..1915
                     /region_name="C2F_Ferlin"
                     /note="C2 domain sixth repeat in Ferlin; cd08374"
                     /db_xref="CDD:176020"
     Region          1925..2043
                     /region_name="Ferlin_C"
                     /note="Ferlin C-terminus; pfam16165"
                     /db_xref="CDD:435183"
     CDS             1..2046
                     /gene="LOC117609906"
                     /coded_by="XM_034336723.2:388..6528"
                     /db_xref="GeneID:117609906"
ORIGIN      
        1 mlprytvnaf eypsitflia rditrrrtik arlnlglsyp sylpkfqfll qsfiwnlgrp
       61 vdeaevlqlt vvsrgvlrne kvlakyglvl qtvaregriv itdslvdlnn kplpavvcfe
      121 irynppdgsc spseimepse imedeqqmli dieqnianle rcldqansgs gsgkrkgswh
      181 speknsrrgf lqrgssmsaa eksperksrs stlksmrsfm klgkqrpprt rscdsgsdtr
      241 elldrrdssc atsnepsran smtslesnas dydsqvsanp sepqeaiarp akkpkpkttd
      301 tgqtalkaqd yqvcitiiea rqlaglnmdp vvcvqigdqr kytsvkestn cpyyneyfvf
      361 dfhmppvmlf dkiitlsvqq srnllranlt lgifkldiat vwaqpdhqfy hkwalltdpd
      421 dvaggpkgyl kcdisvigkg dtvkippkse kdeddiegnl llpdgvpier qrakfivkvy
      481 radglpkmns simanvkkaf tgeikdlvdp yvqvsfaglt gktsvkrhsy apvwneqivf
      541 semfpplcqr ikiqlcdndp vhatvigthf vdlkqisndg ekgflptfgp afihfygsir
      601 dyslidqhst lnvglgegis yrarllvair teisdnieia qseveveptv pinelayarn
      661 eefflfatim datmidkklg dkpmyfelsi gnagnaldgh nesskmcelg pksgtsgdqe
      721 elqevlsgsw qsttpaskpm thdkiyyflp ywddkpclhv rsiwpdyrrr mynsniigki
      781 adkleeglse tqlhvddcsg ekilkstlee lssncnryvs iskssltgpg vgktkldker
      841 tklcqrelen igimsrnlka vvtkssfker lktaqgylqk lkflledpqd slpdvfvwvi
      901 ssgrrvayqr ipgrdliysi vdeecgkscg kvqtmflklp gkkrfgpsgw aiqtklqiym
      961 wmgilkhkky fiqglpkgye ishevknver pralpptiih yvekhkfelr ahmyqarsli
     1021 gsdasglsdp farvicgefc kstqvidetl sptwdelllf ddiliygtae eikkdppsiv
     1081 ieifdqdkvg kseyigrava rphvklases ytppefppsl ewydvsrgaa ragellavfe
     1141 lleypstkdy gfptlpdpke rlpqtttsll dqgpifpvpv girptlskyr ievlfwglrd
     1201 lkrihlmtvd kprvdvecag hilyssvian akknpnfntp ikflelelpe qelyrpplti
     1261 ravdcrsfgr ytlvgthtin sihkymycpq tkkakdaedr lelawptkrd kteqnirkkq
     1321 slvndqstgd fsnfddedgc qdwwtkyfas veamieenke lrkekpmfpa qsngtvpmyl
     1381 eenysqaqmn lsgerspgva tkklfglkst anaarfvski spkqtyqkfp ktallkiypn
     1441 eleaqpqfeq fkewlhtfel yrgkktgdep edesrivgsf kgalkvykwp lprdlidhtv
     1501 mgfdpqygff qgvpsnepih vlvrvyivka ndlhpcdlng kadpyvvlql ggkrisdkeh
     1561 yvskqlnpvf gkyvtidlsi peyvislscr lfprcfeiea tfpqdslltv qvldwdlvga
     1621 ddmigetkid lenrfysrhr atcglakkyd esgynkwrda mkptqilskl ckdgkvdgpv
     1681 yshgkvtigr ktfslaneem eyyvhakgie eqlalavlhq whhfprfgca lvpehvetrp
     1741 lynpekpgie qgklemwvdm fpmdmpspsa sidisprkpk syelriiiwn tddvvledda
     1801 fftgekmsdi yvkgwlkgpe dcqstdihyr sltgegnfnw rfifpfdylv aeekivinrk
     1861 eslfswdete ckiparlelq vwdadhfsad dflgaitldl nrfprgakns klctlsmlkt
     1921 dgtvptvnif kqkrikgwwp fyvkkenedm eltgkveaei hlltkeeaek npagygrnep
     1981 dpldkpkrpd asfmwflnpl ksikyivwhn ykwailkafv tiglialvll ffyaipgysv
     2041 kkllga
//
LOCUS       XP_034192636            1369 aa            linear   INV 03-SEP-2025
DEFINITION  la related protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192636
VERSION     XP_034192636.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192636.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1369
                     /product="la related protein isoform X1"
                     /calculated_mol_wt=155044
     Region          <319..>565
                     /region_name="LHP1"
                     /note="La protein, small RNA-binding pol III transcript
                     stabilizing protein and related La-motif-containing
                     proteins involved in translation [Posttranslational
                     modification, protein turnover, chaperones / Translation,
                     ribosomal structure and biogenesis]; COG5193"
                     /db_xref="CDD:227520"
     Region          501..574
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(507,510..511,516,519..520,522,541..543)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153403"
     Region          1062..1102
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     Region          1103..1141
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     CDS             1..1369
                     /gene="larp"
                     /coded_by="XM_034336745.2:110..4219"
                     /db_xref="GeneID:117609947"
ORIGIN      
        1 maakvasgtq ggtdreegpg vsyasilnpk sgtenrvvnk gnnkenisgq vvqqqsvpik
       61 driasqsltt kgksyqrser rfnpqnklek hygnetpspt eqkqlpvqkk apaeqqqdnv
      121 vgneqmngdv gsdgefqtva pksarrkekl rehqrdhrdq hrhrdhrhpl rghngsners
      181 ykerdrgdrd hfigikdavk ekeetetdse nqsstpvkyv eaplptvnpw tkskmltppa
      241 apvnsvpavp savvppaekq tekekrvlqp qqqgivengi nagnhptivt askdrrkfnq
      301 kasdfsdigd wpslgaqgdk kttvtapkqn gvveqntvke ssgtptenrg kenkeqnhcq
      361 edsdentdvt ekkkkvnkqk wvpleiditk nrgkrerspk yhnqrdksgd dtdsyrnreh
      421 drptyntrgg rggrsyrgrg grggrgayrg sfrhrqdysn ytadymqmhk fghldppymm
      481 pyvgtfyyns tncvldtvtl keyirnqiey yfseenllrd fflrrkmdaq gflpitlias
      541 fhrvqtltmd vglvieaimg sdklelvdgf kvrtkidplk wpildaagnp vfsessdian
      601 psqnevilsa sessdfcpaa rplptipipp vprvlqsnhi vstvgrvses esvsssvgds
      661 lnpdvpefip neltnknnea vapnsdnepg kteeqsdeln sipassnndd setvsssess
      721 kmsttvkcss ptqasevqin gdsqsgvnvw kevrrrvkss hkerneerek idnvksqere
      781 eldfqfdeel dtppptgrhn afsewsedee dnelsdgdvn klliftqttv smptripkhe
      841 ghdrtgdwtt rvkmtqdleq aindglyyye edlwikdgqr ygssssigsy ktvnvisqed
      901 fekmapkmpk kanpevpppp psamddlevs hslptqvpig ldnqerrnrr mdrnrwndks
      961 rlgrsgrrgv vprffavvkd epsvdprtpr krktrhsnnp pvehhvgwim dvrehrprty
     1021 stgssagtsp negylassyg svpqslpafq hpshallken gftqqayhky hsrclkerkr
     1081 lgigqsqemn tlfrfwsffl renfnrtmye efrsiakeda segyryglec lfrfysygle
     1141 krfrhhlyrd fqietihdye tgqlyglekf waflkyykys dqlqvdpklq eylskfksie
     1201 dfrvvepqin emlqgfaahs rsqaakrrnr svsesagdge asptsrvrrl sggsstvtlp
     1261 tlgsennhna lklrvdsvnl sqlrnrtgsf gsgrlghsrr rndtasssts qrdlnkqppr
     1321 srhnsgsctk pqevsksqst ardrlqatfk seqtksvtik tettlisqk
//
LOCUS       XP_034192649            1361 aa            linear   INV 03-SEP-2025
DEFINITION  la related protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192649
VERSION     XP_034192649.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336758.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192649.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1361
                     /product="la related protein isoform X2"
                     /calculated_mol_wt=154194
     Region          <319..>565
                     /region_name="LHP1"
                     /note="La protein, small RNA-binding pol III transcript
                     stabilizing protein and related La-motif-containing
                     proteins involved in translation [Posttranslational
                     modification, protein turnover, chaperones / Translation,
                     ribosomal structure and biogenesis]; COG5193"
                     /db_xref="CDD:227520"
     Region          501..574
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(507,510..511,516,519..520,522,541..543)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153403"
     Region          1054..1094
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     Region          1095..1133
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     CDS             1..1361
                     /gene="larp"
                     /coded_by="XM_034336758.2:110..4195"
                     /db_xref="GeneID:117609947"
ORIGIN      
        1 maakvasgtq ggtdreegpg vsyasilnpk sgtenrvvnk gnnkenisgq vvqqqsvpik
       61 driasqsltt kgksyqrser rfnpqnklek hygnetpspt eqkqlpvqkk apaeqqqdnv
      121 vgneqmngdv gsdgefqtva pksarrkekl rehqrdhrdq hrhrdhrhpl rghngsners
      181 ykerdrgdrd hfigikdavk ekeetetdse nqsstpvkyv eaplptvnpw tkskmltppa
      241 apvnsvpavp savvppaekq tekekrvlqp qqqgivengi nagnhptivt askdrrkfnq
      301 kasdfsdigd wpslgaqgdk kttvtapkqn gvveqntvke ssgtptenrg kenkeqnhcq
      361 edsdentdvt ekkkkvnkqk wvpleiditk nrgkrerspk yhnqrdksgd dtdsyrnreh
      421 drptyntrgg rggrsyrgrg grggrgayrg sfrhrqdysn ytadymqmhk fghldppymm
      481 pyvgtfyyns tncvldtvtl keyirnqiey yfseenllrd fflrrkmdaq gflpitlias
      541 fhrvqtltmd vglvieaimg sdklelvdgf kvrtkidplk wpildaagnp vfsessdian
      601 psqnevilsa sessdfcpaa rplptipipp vprvlqsnhi vstvgrvses esvsssvgds
      661 lnpdvpefip neltnknnea vapnsdnepg kteeqsdeln sipassnndd setvsssess
      721 kmsttvkcss ptqasevqin gdsqsgvnvw kevrrrvkss hkerneerek idnvksqere
      781 eldfqfdeel dtppptgrhn afsewsedee dnelsdgdvn klliftqttv smptripkhe
      841 ghdrtgdwtt rvkmtqdleq aindglyyye edlwikdgqr ygssssigsy ktvnvisqed
      901 fekmapkmpk kanpevpppp psamddlevp igldnqerrn rrmdrnrwnd ksrlgrsgrr
      961 gvvprffavv kdepsvdprt prkrktrhsn nppvehhvgw imdvrehrpr tystgssagt
     1021 spnegylass ygsvpqslpa fqhpshallk engftqqayh kyhsrclker krlgigqsqe
     1081 mntlfrfwsf flrenfnrtm yeefrsiake dasegyrygl eclfrfysyg lekrfrhhly
     1141 rdfqietihd yetgqlygle kfwaflkyyk ysdqlqvdpk lqeylskfks iedfrvvepq
     1201 inemlqgfaa hsrsqaakrr nrsvsesagd geasptsrvr rlsggsstvt lptlgsennh
     1261 nalklrvdsv nlsqlrnrtg sfgsgrlghs rrrndtasss tsqrdlnkqp prsrhnsgsc
     1321 tkpqevsksq stardrlqat fkseqtksvt iktettlisq k
//
LOCUS       XP_034192658            1105 aa            linear   INV 03-SEP-2025
DEFINITION  la related protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034192658
VERSION     XP_034192658.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336767.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192658.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1105
                     /product="la related protein isoform X3"
                     /calculated_mol_wt=125610
     Region          <55..349
                     /region_name="LHP1"
                     /note="La protein, small RNA-binding pol III transcript
                     stabilizing protein and related La-motif-containing
                     proteins involved in translation [Posttranslational
                     modification, protein turnover, chaperones / Translation,
                     ribosomal structure and biogenesis]; COG5193"
                     /db_xref="CDD:227520"
     Region          237..310
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(243,246..247,252,255..256,258,277..279)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153403"
     Region          798..838
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     Region          839..877
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     CDS             1..1105
                     /gene="larp"
                     /coded_by="XM_034336767.2:365..3682"
                     /db_xref="GeneID:117609947"
ORIGIN      
        1 mkinknkgdi vqqnginagn hptivtaskd rrkfnqkasd fsdigdwpsl gaqgdkkttv
       61 tapkqngvve qntvkessgt ptenrgkenk eqnhcqedsd entdvtekkk kvnkqkwvpl
      121 eiditknrgk rerspkyhnq rdksgddtds yrnrehdrpt yntrggrggr syrgrggrgg
      181 rgayrgsfrh rqdysnytad ymqmhkfghl dppymmpyvg tfyynstncv ldtvtlkeyi
      241 rnqieyyfse enllrdfflr rkmdaqgflp itliasfhrv qtltmdvglv ieaimgsdkl
      301 elvdgfkvrt kidplkwpil daagnpvfse ssdianpsqn evilsasess dfcpaarplp
      361 tipippvprv lqsnhivstv grvsesesvs ssvgdslnpd vpefipnelt nknneavapn
      421 sdnepgktee qsdelnsipa ssnnddsetv sssesskmst tvkcssptqa sevqingdsq
      481 sgvnvwkevr rrvksshker neerekidnv ksqereeldf qfdeeldtpp ptgrhnafse
      541 wsedeednel sdgdvnklli ftqttvsmpt ripkheghdr tgdwttrvkm tqdleqaind
      601 glyyyeedlw ikdgqrygss ssigsyktvn visqedfekm apkmpkkanp evpppppsam
      661 ddlevshslp tqvpigldnq errnrrmdrn rwndksrlgr sgrrgvvprf favvkdepsv
      721 dprtprkrkt rhsnnppveh hvgwimdvre hrprtystgs sagtspnegy lassygsvpq
      781 slpafqhpsh allkengftq qayhkyhsrc lkerkrlgig qsqemntlfr fwsfflrenf
      841 nrtmyeefrs iakedasegy rygleclfrf ysyglekrfr hhlyrdfqie tihdyetgql
      901 yglekfwafl kyykysdqlq vdpklqeyls kfksiedfrv vepqinemlq gfaahsrsqa
      961 akrrnrsvse sagdgeaspt srvrrlsggs stvtlptlgs ennhnalklr vdsvnlsqlr
     1021 nrtgsfgsgr lghsrrrndt assstsqrdl nkqpprsrhn sgsctkpqev sksqstardr
     1081 lqatfkseqt ksvtiktett lisqk
//
LOCUS       XP_034192651            1105 aa            linear   INV 03-SEP-2025
DEFINITION  la related protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034192651
VERSION     XP_034192651.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192651.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1105
                     /product="la related protein isoform X3"
                     /calculated_mol_wt=125610
     Region          <55..349
                     /region_name="LHP1"
                     /note="La protein, small RNA-binding pol III transcript
                     stabilizing protein and related La-motif-containing
                     proteins involved in translation [Posttranslational
                     modification, protein turnover, chaperones / Translation,
                     ribosomal structure and biogenesis]; COG5193"
                     /db_xref="CDD:227520"
     Region          237..310
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(243,246..247,252,255..256,258,277..279)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153403"
     Region          798..838
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     Region          839..877
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     CDS             1..1105
                     /gene="larp"
                     /coded_by="XM_034336760.2:847..4164"
                     /db_xref="GeneID:117609947"
ORIGIN      
        1 mkinknkgdi vqqnginagn hptivtaskd rrkfnqkasd fsdigdwpsl gaqgdkkttv
       61 tapkqngvve qntvkessgt ptenrgkenk eqnhcqedsd entdvtekkk kvnkqkwvpl
      121 eiditknrgk rerspkyhnq rdksgddtds yrnrehdrpt yntrggrggr syrgrggrgg
      181 rgayrgsfrh rqdysnytad ymqmhkfghl dppymmpyvg tfyynstncv ldtvtlkeyi
      241 rnqieyyfse enllrdfflr rkmdaqgflp itliasfhrv qtltmdvglv ieaimgsdkl
      301 elvdgfkvrt kidplkwpil daagnpvfse ssdianpsqn evilsasess dfcpaarplp
      361 tipippvprv lqsnhivstv grvsesesvs ssvgdslnpd vpefipnelt nknneavapn
      421 sdnepgktee qsdelnsipa ssnnddsetv sssesskmst tvkcssptqa sevqingdsq
      481 sgvnvwkevr rrvksshker neerekidnv ksqereeldf qfdeeldtpp ptgrhnafse
      541 wsedeednel sdgdvnklli ftqttvsmpt ripkheghdr tgdwttrvkm tqdleqaind
      601 glyyyeedlw ikdgqrygss ssigsyktvn visqedfekm apkmpkkanp evpppppsam
      661 ddlevshslp tqvpigldnq errnrrmdrn rwndksrlgr sgrrgvvprf favvkdepsv
      721 dprtprkrkt rhsnnppveh hvgwimdvre hrprtystgs sagtspnegy lassygsvpq
      781 slpafqhpsh allkengftq qayhkyhsrc lkerkrlgig qsqemntlfr fwsfflrenf
      841 nrtmyeefrs iakedasegy rygleclfrf ysyglekrfr hhlyrdfqie tihdyetgql
      901 yglekfwafl kyykysdqlq vdpklqeyls kfksiedfrv vepqinemlq gfaahsrsqa
      961 akrrnrsvse sagdgeaspt srvrrlsggs stvtlptlgs ennhnalklr vdsvnlsqlr
     1021 nrtgsfgsgr lghsrrrndt assstsqrdl nkqpprsrhn sgsctkpqev sksqstardr
     1081 lqatfkseqt ksvtiktett lisqk
//
LOCUS       XP_076546855            1105 aa            linear   INV 03-SEP-2025
DEFINITION  la related protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546855
VERSION     XP_076546855.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690740.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1105
                     /product="la related protein isoform X3"
                     /calculated_mol_wt=125610
     Region          <55..349
                     /region_name="LHP1"
                     /note="La protein, small RNA-binding pol III transcript
                     stabilizing protein and related La-motif-containing
                     proteins involved in translation [Posttranslational
                     modification, protein turnover, chaperones / Translation,
                     ribosomal structure and biogenesis]; COG5193"
                     /db_xref="CDD:227520"
     Region          237..310
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(243,246..247,252,255..256,258,277..279)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153403"
     Region          798..838
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     Region          839..877
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     CDS             1..1105
                     /gene="larp"
                     /coded_by="XM_076690740.1:566..3883"
                     /db_xref="GeneID:117609947"
ORIGIN      
        1 mkinknkgdi vqqnginagn hptivtaskd rrkfnqkasd fsdigdwpsl gaqgdkkttv
       61 tapkqngvve qntvkessgt ptenrgkenk eqnhcqedsd entdvtekkk kvnkqkwvpl
      121 eiditknrgk rerspkyhnq rdksgddtds yrnrehdrpt yntrggrggr syrgrggrgg
      181 rgayrgsfrh rqdysnytad ymqmhkfghl dppymmpyvg tfyynstncv ldtvtlkeyi
      241 rnqieyyfse enllrdfflr rkmdaqgflp itliasfhrv qtltmdvglv ieaimgsdkl
      301 elvdgfkvrt kidplkwpil daagnpvfse ssdianpsqn evilsasess dfcpaarplp
      361 tipippvprv lqsnhivstv grvsesesvs ssvgdslnpd vpefipnelt nknneavapn
      421 sdnepgktee qsdelnsipa ssnnddsetv sssesskmst tvkcssptqa sevqingdsq
      481 sgvnvwkevr rrvksshker neerekidnv ksqereeldf qfdeeldtpp ptgrhnafse
      541 wsedeednel sdgdvnklli ftqttvsmpt ripkheghdr tgdwttrvkm tqdleqaind
      601 glyyyeedlw ikdgqrygss ssigsyktvn visqedfekm apkmpkkanp evpppppsam
      661 ddlevshslp tqvpigldnq errnrrmdrn rwndksrlgr sgrrgvvprf favvkdepsv
      721 dprtprkrkt rhsnnppveh hvgwimdvre hrprtystgs sagtspnegy lassygsvpq
      781 slpafqhpsh allkengftq qayhkyhsrc lkerkrlgig qsqemntlfr fwsfflrenf
      841 nrtmyeefrs iakedasegy rygleclfrf ysyglekrfr hhlyrdfqie tihdyetgql
      901 yglekfwafl kyykysdqlq vdpklqeyls kfksiedfrv vepqinemlq gfaahsrsqa
      961 akrrnrsvse sagdgeaspt srvrrlsggs stvtlptlgs ennhnalklr vdsvnlsqlr
     1021 nrtgsfgsgr lghsrrrndt assstsqrdl nkqpprsrhn sgsctkpqev sksqstardr
     1081 lqatfkseqt ksvtiktett lisqk
//
LOCUS       XP_076546856            1105 aa            linear   INV 03-SEP-2025
DEFINITION  la related protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076546856
VERSION     XP_076546856.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690741.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1105
                     /product="la related protein isoform X3"
                     /calculated_mol_wt=125610
     Region          <55..349
                     /region_name="LHP1"
                     /note="La protein, small RNA-binding pol III transcript
                     stabilizing protein and related La-motif-containing
                     proteins involved in translation [Posttranslational
                     modification, protein turnover, chaperones / Translation,
                     ribosomal structure and biogenesis]; COG5193"
                     /db_xref="CDD:227520"
     Region          237..310
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(243,246..247,252,255..256,258,277..279)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153403"
     Region          798..838
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     Region          839..877
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     CDS             1..1105
                     /gene="larp"
                     /coded_by="XM_076690741.1:228..3545"
                     /db_xref="GeneID:117609947"
ORIGIN      
        1 mkinknkgdi vqqnginagn hptivtaskd rrkfnqkasd fsdigdwpsl gaqgdkkttv
       61 tapkqngvve qntvkessgt ptenrgkenk eqnhcqedsd entdvtekkk kvnkqkwvpl
      121 eiditknrgk rerspkyhnq rdksgddtds yrnrehdrpt yntrggrggr syrgrggrgg
      181 rgayrgsfrh rqdysnytad ymqmhkfghl dppymmpyvg tfyynstncv ldtvtlkeyi
      241 rnqieyyfse enllrdfflr rkmdaqgflp itliasfhrv qtltmdvglv ieaimgsdkl
      301 elvdgfkvrt kidplkwpil daagnpvfse ssdianpsqn evilsasess dfcpaarplp
      361 tipippvprv lqsnhivstv grvsesesvs ssvgdslnpd vpefipnelt nknneavapn
      421 sdnepgktee qsdelnsipa ssnnddsetv sssesskmst tvkcssptqa sevqingdsq
      481 sgvnvwkevr rrvksshker neerekidnv ksqereeldf qfdeeldtpp ptgrhnafse
      541 wsedeednel sdgdvnklli ftqttvsmpt ripkheghdr tgdwttrvkm tqdleqaind
      601 glyyyeedlw ikdgqrygss ssigsyktvn visqedfekm apkmpkkanp evpppppsam
      661 ddlevshslp tqvpigldnq errnrrmdrn rwndksrlgr sgrrgvvprf favvkdepsv
      721 dprtprkrkt rhsnnppveh hvgwimdvre hrprtystgs sagtspnegy lassygsvpq
      781 slpafqhpsh allkengftq qayhkyhsrc lkerkrlgig qsqemntlfr fwsfflrenf
      841 nrtmyeefrs iakedasegy rygleclfrf ysyglekrfr hhlyrdfqie tihdyetgql
      901 yglekfwafl kyykysdqlq vdpklqeyls kfksiedfrv vepqinemlq gfaahsrsqa
      961 akrrnrsvse sagdgeaspt srvrrlsggs stvtlptlgs ennhnalklr vdsvnlsqlr
     1021 nrtgsfgsgr lghsrrrndt assstsqrdl nkqpprsrhn sgsctkpqev sksqstardr
     1081 lqatfkseqt ksvtiktett lisqk
//
LOCUS       XP_034192668            1099 aa            linear   INV 03-SEP-2025
DEFINITION  la related protein isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034192668
VERSION     XP_034192668.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336777.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192668.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1099
                     /product="la related protein isoform X4"
                     /calculated_mol_wt=124997
     Region          <49..343
                     /region_name="LHP1"
                     /note="La protein, small RNA-binding pol III transcript
                     stabilizing protein and related La-motif-containing
                     proteins involved in translation [Posttranslational
                     modification, protein turnover, chaperones / Translation,
                     ribosomal structure and biogenesis]; COG5193"
                     /db_xref="CDD:227520"
     Region          231..304
                     /region_name="LAM"
                     /note="LA motif RNA-binding domain; cl02755"
                     /db_xref="CDD:470666"
     Site            order(237,240..241,246,249..250,252,271..273)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:153403"
     Region          792..832
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     Region          833..871
                     /region_name="DM15"
                     /note="Tandem repeat in fly CG14066 (La related protein),
                     human KIAA0731 and worm R144.7. Unknown function;
                     smart00684"
                     /db_xref="CDD:128927"
     CDS             1..1099
                     /gene="larp"
                     /coded_by="XM_034336777.2:1106..4405"
                     /db_xref="GeneID:117609947"
ORIGIN      
        1 mihfnnqngi nagnhptivt askdrrkfnq kasdfsdigd wpslgaqgdk kttvtapkqn
       61 gvveqntvke ssgtptenrg kenkeqnhcq edsdentdvt ekkkkvnkqk wvpleiditk
      121 nrgkrerspk yhnqrdksgd dtdsyrnreh drptyntrgg rggrsyrgrg grggrgayrg
      181 sfrhrqdysn ytadymqmhk fghldppymm pyvgtfyyns tncvldtvtl keyirnqiey
      241 yfseenllrd fflrrkmdaq gflpitlias fhrvqtltmd vglvieaimg sdklelvdgf
      301 kvrtkidplk wpildaagnp vfsessdian psqnevilsa sessdfcpaa rplptipipp
      361 vprvlqsnhi vstvgrvses esvsssvgds lnpdvpefip neltnknnea vapnsdnepg
      421 kteeqsdeln sipassnndd setvsssess kmsttvkcss ptqasevqin gdsqsgvnvw
      481 kevrrrvkss hkerneerek idnvksqere eldfqfdeel dtppptgrhn afsewsedee
      541 dnelsdgdvn klliftqttv smptripkhe ghdrtgdwtt rvkmtqdleq aindglyyye
      601 edlwikdgqr ygssssigsy ktvnvisqed fekmapkmpk kanpevpppp psamddlevs
      661 hslptqvpig ldnqerrnrr mdrnrwndks rlgrsgrrgv vprffavvkd epsvdprtpr
      721 krktrhsnnp pvehhvgwim dvrehrprty stgssagtsp negylassyg svpqslpafq
      781 hpshallken gftqqayhky hsrclkerkr lgigqsqemn tlfrfwsffl renfnrtmye
      841 efrsiakeda segyryglec lfrfysygle krfrhhlyrd fqietihdye tgqlyglekf
      901 waflkyykys dqlqvdpklq eylskfksie dfrvvepqin emlqgfaahs rsqaakrrnr
      961 svsesagdge asptsrvrrl sggsstvtlp tlgsennhna lklrvdsvnl sqlrnrtgsf
     1021 gsgrlghsrr rndtasssts qrdlnkqppr srhnsgsctk pqevsksqst ardrlqatfk
     1081 seqtksvtik tettlisqk
//
LOCUS       XP_034192681             892 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609975 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192681
VERSION     XP_034192681.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336790.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192681.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..892
                     /product="uncharacterized protein LOC117609975 isoform X1"
                     /calculated_mol_wt=99418
     CDS             1..892
                     /gene="LOC117609975"
                     /coded_by="XM_034336790.2:385..3063"
                     /db_xref="GeneID:117609975"
ORIGIN      
        1 mserkvdaei vqkqcindts lvdeaeiidv safdfddtgs wlyiptryqt estvqdlytw
       61 lrsepelnls anrksvdtrt ftrskkrstk tcfesifetl sspvaniwkt snfsnveedl
      121 nkysdlptan kdeavppmlk mapitnvsll ndeavvtsgq esmevflnms hskgidsfin
      181 lmepglsdpl mnvsqpsify ssitslsrdd slhnadcgre eefsnsihmy nkeidkhkda
      241 knsnmnetfs kdnesyclsp tdetftngsa vmnkvelnqt ydteavsaei velnsdkdks
      301 stnslssilv nkddgnakfl qprgtekkds sslnttypsn aqkvgnplal dstyspikve
      361 nvpagvtstf rsdikrdkgq pfsletnasc stsknaiess aglanstfsl dndvldspfk
      421 lppgyqstpi smdtlnstyk asnkqlescr vynttwssnf kaptslrkel laeihrsgdh
      481 rldctynhts kehqsknskt aivekipsqn vksdipienk yntykkesss vdkikpqtei
      541 netktcvhkl qekkyytftk knsahggktd menaeslenm dstfvkplpk lqkqkqhipr
      601 mlsklpqflq ksnpnlvsns lktvnmtgyt nastfgymkg sqpnivrdve ksltnklysl
      661 gkmksgseqr llelnsgvge lqllgaggst esiestqsah sapdlddrls tcsdsshnsc
      721 ttqpmnieql qqivrmqees lrqdtrpqlk rvlentwidm ktdlhsaivk ngvdgcegdd
      781 lplsidftfk tsspilsptr ssqsintdgq stetvikqqn dtevvkkvea fnkpmmkven
      841 ktrlrqptnw nsgnrppnvm saiprppsri paprfakpiv kniqgdikrg ym
//
LOCUS       XP_034192691             892 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609975 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192691
VERSION     XP_034192691.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336800.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192691.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..892
                     /product="uncharacterized protein LOC117609975 isoform X1"
                     /calculated_mol_wt=99418
     CDS             1..892
                     /gene="LOC117609975"
                     /coded_by="XM_034336800.2:385..3063"
                     /db_xref="GeneID:117609975"
ORIGIN      
        1 mserkvdaei vqkqcindts lvdeaeiidv safdfddtgs wlyiptryqt estvqdlytw
       61 lrsepelnls anrksvdtrt ftrskkrstk tcfesifetl sspvaniwkt snfsnveedl
      121 nkysdlptan kdeavppmlk mapitnvsll ndeavvtsgq esmevflnms hskgidsfin
      181 lmepglsdpl mnvsqpsify ssitslsrdd slhnadcgre eefsnsihmy nkeidkhkda
      241 knsnmnetfs kdnesyclsp tdetftngsa vmnkvelnqt ydteavsaei velnsdkdks
      301 stnslssilv nkddgnakfl qprgtekkds sslnttypsn aqkvgnplal dstyspikve
      361 nvpagvtstf rsdikrdkgq pfsletnasc stsknaiess aglanstfsl dndvldspfk
      421 lppgyqstpi smdtlnstyk asnkqlescr vynttwssnf kaptslrkel laeihrsgdh
      481 rldctynhts kehqsknskt aivekipsqn vksdipienk yntykkesss vdkikpqtei
      541 netktcvhkl qekkyytftk knsahggktd menaeslenm dstfvkplpk lqkqkqhipr
      601 mlsklpqflq ksnpnlvsns lktvnmtgyt nastfgymkg sqpnivrdve ksltnklysl
      661 gkmksgseqr llelnsgvge lqllgaggst esiestqsah sapdlddrls tcsdsshnsc
      721 ttqpmnieql qqivrmqees lrqdtrpqlk rvlentwidm ktdlhsaivk ngvdgcegdd
      781 lplsidftfk tsspilsptr ssqsintdgq stetvikqqn dtevvkkvea fnkpmmkven
      841 ktrlrqptnw nsgnrppnvm saiprppsri paprfakpiv kniqgdikrg ym
//
LOCUS       XP_034192701             755 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609975 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192701
VERSION     XP_034192701.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336810.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192701.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..755
                     /product="uncharacterized protein LOC117609975 isoform X2"
                     /calculated_mol_wt=83735
     CDS             1..755
                     /gene="LOC117609975"
                     /coded_by="XM_034336810.2:430..2697"
                     /db_xref="GeneID:117609975"
ORIGIN      
        1 mlkmapitnv sllndeavvt sgqesmevfl nmshskgids finlmepgls dplmnvsqps
       61 ifyssitsls rddslhnadc greeefsnsi hmynkeidkh kdaknsnmne tfskdnesyc
      121 lsptdetftn gsavmnkvel nqtydteavs aeivelnsdk dksstnslss ilvnkddgna
      181 kflqprgtek kdssslntty psnaqkvgnp laldstyspi kvenvpagvt stfrsdikrd
      241 kgqpfsletn ascstsknai essaglanst fsldndvlds pfklppgyqs tpismdtlns
      301 tykasnkqle scrvynttws snfkaptslr kellaeihrs gdhrldctyn htskehqskn
      361 sktaivekip sqnvksdipi enkyntykke sssvdkikpq teinetktcv hklqekkyyt
      421 ftkknsahgg ktdmenaesl enmdstfvkp lpklqkqkqh iprmlsklpq flqksnpnlv
      481 snslktvnmt gytnastfgy mkgsqpnivr dveksltnkl yslgkmksgs eqrllelnsg
      541 vgelqllgag gstesiestq sahsapdldd rlstcsdssh nscttqpmni eqlqqivrmq
      601 eeslrqdtrp qlkrvlentw idmktdlhsa ivkngvdgce gddlplsidf tfktsspils
      661 ptrssqsint dgqstetvik qqndtevvkk veafnkpmmk venktrlrqp tnwnsgnrpp
      721 nvmsaiprpp sripaprfak pivkniqgdi krgym
//
LOCUS       XP_034192714             836 aa            linear   INV 03-SEP-2025
DEFINITION  O-acyltransferase like protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192714
VERSION     XP_034192714.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336823.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192714.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..836
                     /product="O-acyltransferase like protein isoform X1"
                     /calculated_mol_wt=95858
     Region          58..181
                     /region_name="NRF"
                     /note="Nose resistant-to-fluoxetine protein, N-terminal
                     domain; pfam20146"
                     /db_xref="CDD:466307"
     Region          278..665
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..836
                     /gene="LOC117609987"
                     /coded_by="XM_034336823.2:173..2683"
                     /db_xref="GeneID:117609987"
ORIGIN      
        1 mlrsilfhrh lsylcpclsi lmllglvvpe mtadkpetvl fdllakpfap sdgasveclk
       61 dseiylkela ryapwalqmy dasvkippgv itgnyqqlgn ydgcllvksg hgftgkacsa
      121 kvnfeiakdn gkprepdmgd llvnaaiasg ygkwksgrtv fyewmwcvps scnhtdiqea
      181 lelaldplkv edrvdmvvnv scrtaetdqp vidvtdriyi svlaiflvii iastsydiak
      241 qghlstlnrd rkdillssfs iytngknllk tdrrtdsikc ldglryisic wiiyghthyt
      301 evvgvkmnln eisqmhtnwa nmfvlnaniv tdtfflisgv lmaytemmkn ekdikrqfnv
      361 iglylhrylr ltpayammig fyttifykfg sgphwdqwvg anrdycrenw wtnllyvnny
      421 vnldrmcmsq swylatdmql vwlspiflyp mlkftrkiff wlvfafgiva silipflitf
      481 sleltgtmly ykdiqavadv yvqiytkvys rygsyiigla vgyilykyrl rvlkihrmyv
      541 ifgwlvavfs glfvivfprw myyddhpydr leasfyagfh rqifalsvsw iifcsvhgya
      601 givgqllswk gwvpfsklty taylchyvfl lteagsvrtt gilstmgilr sffsnlcltl
      661 llsavwtlcf empfmtldrt flfyikrqsk lgckpnqeqi fgsldsskei yrsteessat
      721 isqtsfdsnd ssnesvydsa gnvnyqqgif dtncsedlkh dredkcsfif vtgesknntw
      781 pkirnihqnn gfdsdsddsl klgndenrld rsynsiisdd lsrkdrsrhs etvnvs
//
LOCUS       XP_034192719             699 aa            linear   INV 03-SEP-2025
DEFINITION  nose resistant to fluoxetine protein 6 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192719
VERSION     XP_034192719.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336828.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192719.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..699
                     /product="nose resistant to fluoxetine protein 6 isoform
                     X2"
                     /calculated_mol_wt=80799
     Region          141..528
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..699
                     /gene="LOC117609987"
                     /coded_by="XM_034336828.2:835..2934"
                     /db_xref="GeneID:117609987"
ORIGIN      
        1 mgdllvnaai asgygkwksg rtvfyewmwc vpsscnhtdi qealelaldp lkvedrvdmv
       61 vnvscrtaet dqpvidvtdr iyisvlaifl viiiastsyd iakqghlstl nrdrkdills
      121 sfsiytngkn llktdrrtds ikcldglryi sicwiiyght hytevvgvkm nlneisqmht
      181 nwanmfvlna nivtdtffli sgvlmaytem mknekdikrq fnviglylhr ylrltpayam
      241 migfyttify kfgsgphwdq wvganrdycr enwwtnllyv nnyvnldrmc msqswylatd
      301 mqlvwlspif lypmlkftrk iffwlvfafg ivasilipfl itfsleltgt mlyykdiqav
      361 advyvqiytk vysrygsyii glavgyilyk yrlrvlkihr myvifgwlva vfsglfvivf
      421 prwmyyddhp ydrleasfya gfhrqifals vswiifcsvh gyagivgqll swkgwvpfsk
      481 ltytaylchy vfllteagsv rttgilstmg ilrsffsnlc ltlllsavwt lcfempfmtl
      541 drtflfyikr qsklgckpnq eqifgsldss keiyrstees satisqtsfd sndssnesvy
      601 dsagnvnyqq gifdtncsed lkhdredkcs fifvtgeskn ntwpkirnih qnngfdsdsd
      661 dslklgnden rldrsynsii sddlsrkdrs rhsetvnvs
//
LOCUS       XP_076546857             699 aa            linear   INV 03-SEP-2025
DEFINITION  nose resistant to fluoxetine protein 6 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076546857
VERSION     XP_076546857.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690742.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..699
                     /product="nose resistant to fluoxetine protein 6 isoform
                     X2"
                     /calculated_mol_wt=80799
     Region          141..528
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..699
                     /gene="LOC117609987"
                     /coded_by="XM_076690742.1:301..2400"
                     /db_xref="GeneID:117609987"
ORIGIN      
        1 mgdllvnaai asgygkwksg rtvfyewmwc vpsscnhtdi qealelaldp lkvedrvdmv
       61 vnvscrtaet dqpvidvtdr iyisvlaifl viiiastsyd iakqghlstl nrdrkdills
      121 sfsiytngkn llktdrrtds ikcldglryi sicwiiyght hytevvgvkm nlneisqmht
      181 nwanmfvlna nivtdtffli sgvlmaytem mknekdikrq fnviglylhr ylrltpayam
      241 migfyttify kfgsgphwdq wvganrdycr enwwtnllyv nnyvnldrmc msqswylatd
      301 mqlvwlspif lypmlkftrk iffwlvfafg ivasilipfl itfsleltgt mlyykdiqav
      361 advyvqiytk vysrygsyii glavgyilyk yrlrvlkihr myvifgwlva vfsglfvivf
      421 prwmyyddhp ydrleasfya gfhrqifals vswiifcsvh gyagivgqll swkgwvpfsk
      481 ltytaylchy vfllteagsv rttgilstmg ilrsffsnlc ltlllsavwt lcfempfmtl
      541 drtflfyikr qsklgckpnq eqifgsldss keiyrstees satisqtsfd sndssnesvy
      601 dsagnvnyqq gifdtncsed lkhdredkcs fifvtgeskn ntwpkirnih qnngfdsdsd
      661 dslklgnden rldrsynsii sddlsrkdrs rhsetvnvs
//
LOCUS       XP_034192728             672 aa            linear   INV 03-SEP-2025
DEFINITION  nose resistant to fluoxetine protein 6 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034192728
VERSION     XP_034192728.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336837.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192728.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..672
                     /product="nose resistant to fluoxetine protein 6 isoform
                     X3"
                     /calculated_mol_wt=77797
     Region          114..501
                     /region_name="Acyl_transf_3"
                     /note="Acyltransferase family; pfam01757"
                     /db_xref="CDD:426413"
     CDS             1..672
                     /gene="LOC117609987"
                     /coded_by="XM_034336837.2:939..2957"
                     /db_xref="GeneID:117609987"
ORIGIN      
        1 mwcvpsscnh tdiqealela ldplkvedrv dmvvnvscrt aetdqpvidv tdriyisvla
       61 iflviiiast sydiakqghl stlnrdrkdi llssfsiytn gknllktdrr tdsikcldgl
      121 ryisicwiiy ghthytevvg vkmnlneisq mhtnwanmfv lnanivtdtf flisgvlmay
      181 temmknekdi krqfnvigly lhrylrltpa yammigfytt ifykfgsgph wdqwvganrd
      241 ycrenwwtnl lyvnnyvnld rmcmsqswyl atdmqlvwls piflypmlkf trkiffwlvf
      301 afgivasili pflitfslel tgtmlyykdi qavadvyvqi ytkvysrygs yiiglavgyi
      361 lykyrlrvlk ihrmyvifgw lvavfsglfv ivfprwmyyd dhpydrleas fyagfhrqif
      421 alsvswiifc svhgyagivg qllswkgwvp fskltytayl chyvflltea gsvrttgils
      481 tmgilrsffs nlcltlllsa vwtlcfempf mtldrtflfy ikrqsklgck pnqeqifgsl
      541 dsskeiyrst eessatisqt sfdsndssne svydsagnvn yqqgifdtnc sedlkhdred
      601 kcsfifvtge sknntwpkir nihqnngfds dsddslklgn denrldrsyn siisddlsrk
      661 drsrhsetvn vs
//
LOCUS       XP_034192738             587 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192738
VERSION     XP_034192738.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336847.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..587
                     /product="serine/threonine-protein kinase tricornered
                     isoform X1"
                     /calculated_mol_wt=66769
     Region          141..205
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(141,144..145,147..149,151..152,155..156,159,162,191,
                     194..196,198..199,202..203)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          208..573
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(216..220,224,237,239,271,287..288,290,294,296,333,
                     335,337..338,340,350..351,354,410..415,442,448,451)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(216..220,224,237,239,271,288..290,294,333,335,
                     337..338,340,350..351)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(220,294,296,333,335,337,354,410..415,442,448,451)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(350..362,406..415)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            567..572
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..587
                     /gene="trc"
                     /coded_by="XM_034336847.2:2032..3795"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 mtfpfcgfvf dssderarqm pvasedwvph pakitssitt vkgatiqstt tawmspysra
       61 apserstpnn nnnnnnhnnn nnnnngvvil egcrppaatt arrflrwfsr lgqppmgksg
      121 vlemaatest irfsghtldk atkakvtlen yysnliaqhi erkqrlakle eslkdeglse
      181 qqkqekrlqh aqketeflrl krsrlgvedf eplkvigrga fgevrlvqkk dtghvyamki
      241 lrkadmleke qvahvraerd vlveadhqwv vkmyysfqdp inlylimefl pggdmmtllm
      301 kkdtlseect qfyisetala idsihklgfi hrdikpdnll ldarghikls dfglctglkk
      361 shrtdfyrdl sqakpsdfmt scgsgsggam dskrraeswk rnrralayst vgtpdyiape
      421 vflqtgygpa cdcwslgvim yemligyppf csenpqetyr kvmnwretlv fppevpisee
      481 akdtiirfcc eadrrlgaqr gieelklapf frgvdwehir erpaaipvev rsiddtsnfd
      541 efpdvkleip sapmpqdgev iykdwvfiny tfkrfegltq rgtptkk
//
LOCUS       XP_034192746             522 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192746
VERSION     XP_034192746.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336855.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="serine/threonine-protein kinase tricornered
                     isoform X2"
                     /calculated_mol_wt=59814
     Region          76..140
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(76,79..80,82..84,86..87,90..91,94,97,126,129..131,
                     133..134,137..138)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          143..508
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(151..155,159,172,174,206,222..223,225,229,231,268,
                     270,272..273,275,285..286,289,345..350,377,383,386)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(151..155,159,172,174,206,223..225,229,268,270,
                     272..273,275,285..286)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(155,229,231,268,270,272,289,345..350,377,383,386)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(285..297,341..350)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            502..507
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..522
                     /gene="trc"
                     /coded_by="XM_034336855.2:22..1590"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 mspvnaftvt lkiptyntrv afdfsvepfc grkdcndira lhdrspqplf nklqgvlema
       61 atestirfsg htldkatkak vtlenyysnl iaqhierkqr lakleeslkd eglseqqkqe
      121 krlqhaqket eflrlkrsrl gvedfeplkv igrgafgevr lvqkkdtghv yamkilrkad
      181 mlekeqvahv raerdvlvea dhqwvvkmyy sfqdpinlyl imeflpggdm mtllmkkdtl
      241 seectqfyis etalaidsih klgfihrdik pdnllldarg hiklsdfglc tglkkshrtd
      301 fyrdlsqakp sdfmtscgsg sggamdskrr aeswkrnrra laystvgtpd yiapevflqt
      361 gygpacdcws lgvimyemli gyppfcsenp qetyrkvmnw retlvfppev piseeakdti
      421 irfcceadrr lgaqrgieel klapffrgvd wehirerpaa ipvevrsidd tsnfdefpdv
      481 kleipsapmp qdgeviykdw vfinytfkrf egltqrgtpt kk
//
LOCUS       XP_034192756             513 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192756
VERSION     XP_034192756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..513
                     /product="serine/threonine-protein kinase tricornered
                     isoform X3"
                     /calculated_mol_wt=58492
     Region          67..131
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(67,70..71,73..75,77..78,81..82,85,88,117,120..122,
                     124..125,128..129)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          134..499
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(142..146,150,163,165,197,213..214,216,220,222,259,
                     261,263..264,266,276..277,280,336..341,368,374,377)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(142..146,150,163,165,197,214..216,220,259,261,
                     263..264,266,276..277)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(146,220,222,259,261,263,280,336..341,368,374,377)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(276..288,332..341)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            493..498
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..513
                     /gene="trc"
                     /coded_by="XM_034336865.2:138..1679"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 mceeatevet hhqqtaattm gvaedetpss ltshpnpnvn npnqegvlem aatestirfs
       61 ghtldkatka kvtlenyysn liaqhierkq rlakleeslk deglseqqkq ekrlqhaqke
      121 teflrlkrsr lgvedfeplk vigrgafgev rlvqkkdtgh vyamkilrka dmlekeqvah
      181 vraerdvlve adhqwvvkmy ysfqdpinly limeflpggd mmtllmkkdt lseectqfyi
      241 setalaidsi hklgfihrdi kpdnllldar ghiklsdfgl ctglkkshrt dfyrdlsqak
      301 psdfmtscgs gsggamdskr raeswkrnrr alaystvgtp dyiapevflq tgygpacdcw
      361 slgvimyeml igyppfcsen pqetyrkvmn wretlvfppe vpiseeakdt iirfcceadr
      421 rlgaqrgiee lklapffrgv dwehirerpa aipvevrsid dtsnfdefpd vkleipsapm
      481 pqdgeviykd wvfinytfkr fegltqrgtp tkk
//
LOCUS       XP_034192765             497 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192765
VERSION     XP_034192765.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336874.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="serine/threonine-protein kinase tricornered
                     isoform X4"
                     /calculated_mol_wt=57026
     Region          51..115
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(51,54..55,57..59,61..62,65..66,69,72,101,104..106,
                     108..109,112..113)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          118..483
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(126..130,134,147,149,181,197..198,200,204,206,243,
                     245,247..248,250,260..261,264,320..325,352,358,361)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(126..130,134,147,149,181,198..200,204,243,245,
                     247..248,250,260..261)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(130,204,206,243,245,247,264,320..325,352,358,361)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(260..272,316..325)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            477..482
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..497
                     /gene="trc"
                     /coded_by="XM_034336874.2:1311..2804"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 mydselfktl wtkigqmcsy itcaksylsg vlemaatest irfsghtldk atkakvtlen
       61 yysnliaqhi erkqrlakle eslkdeglse qqkqekrlqh aqketeflrl krsrlgvedf
      121 eplkvigrga fgevrlvqkk dtghvyamki lrkadmleke qvahvraerd vlveadhqwv
      181 vkmyysfqdp inlylimefl pggdmmtllm kkdtlseect qfyisetala idsihklgfi
      241 hrdikpdnll ldarghikls dfglctglkk shrtdfyrdl sqakpsdfmt scgsgsggam
      301 dskrraeswk rnrralayst vgtpdyiape vflqtgygpa cdcwslgvim yemligyppf
      361 csenpqetyr kvmnwretlv fppevpisee akdtiirfcc eadrrlgaqr gieelklapf
      421 frgvdwehir erpaaipvev rsiddtsnfd efpdvkleip sapmpqdgev iykdwvfiny
      481 tfkrfegltq rgtptkk
//
LOCUS       XP_034192774             497 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192774
VERSION     XP_034192774.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336883.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="serine/threonine-protein kinase tricornered
                     isoform X4"
                     /calculated_mol_wt=57026
     Region          51..115
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(51,54..55,57..59,61..62,65..66,69,72,101,104..106,
                     108..109,112..113)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          118..483
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(126..130,134,147,149,181,197..198,200,204,206,243,
                     245,247..248,250,260..261,264,320..325,352,358,361)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(126..130,134,147,149,181,198..200,204,243,245,
                     247..248,250,260..261)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(130,204,206,243,245,247,264,320..325,352,358,361)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(260..272,316..325)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            477..482
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..497
                     /gene="trc"
                     /coded_by="XM_034336883.2:336..1829"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 mydselfktl wtkigqmcsy itcaksylsg vlemaatest irfsghtldk atkakvtlen
       61 yysnliaqhi erkqrlakle eslkdeglse qqkqekrlqh aqketeflrl krsrlgvedf
      121 eplkvigrga fgevrlvqkk dtghvyamki lrkadmleke qvahvraerd vlveadhqwv
      181 vkmyysfqdp inlylimefl pggdmmtllm kkdtlseect qfyisetala idsihklgfi
      241 hrdikpdnll ldarghikls dfglctglkk shrtdfyrdl sqakpsdfmt scgsgsggam
      301 dskrraeswk rnrralayst vgtpdyiape vflqtgygpa cdcwslgvim yemligyppf
      361 csenpqetyr kvmnwretlv fppevpisee akdtiirfcc eadrrlgaqr gieelklapf
      421 frgvdwehir erpaaipvev rsiddtsnfd efpdvkleip sapmpqdgev iykdwvfiny
      481 tfkrfegltq rgtptkk
//
LOCUS       XP_034192778             494 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192778
VERSION     XP_034192778.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336887.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="serine/threonine-protein kinase tricornered
                     isoform X5"
                     /calculated_mol_wt=56393
     Region          48..112
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(48,51..52,54..56,58..59,62..63,66,69,98,101..103,
                     105..106,109..110)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          115..480
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(123..127,131,144,146,178,194..195,197,201,203,240,
                     242,244..245,247,257..258,261,317..322,349,355,358)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(123..127,131,144,146,178,195..197,201,240,242,
                     244..245,247,257..258)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(127,201,203,240,242,244,261,317..322,349,355,358)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(257..269,313..322)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            474..479
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..494
                     /gene="trc"
                     /coded_by="XM_034336887.2:197..1681"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 mgvaedetps sltshpnpnv nnpnqegvle maatestirf sghtldkatk akvtlenyys
       61 nliaqhierk qrlakleesl kdeglseqqk qekrlqhaqk eteflrlkrs rlgvedfepl
      121 kvigrgafge vrlvqkkdtg hvyamkilrk admlekeqva hvraerdvlv eadhqwvvkm
      181 yysfqdpinl ylimeflpgg dmmtllmkkd tlseectqfy isetalaids ihklgfihrd
      241 ikpdnlllda rghiklsdfg lctglkkshr tdfyrdlsqa kpsdfmtscg sgsggamdsk
      301 rraeswkrnr ralaystvgt pdyiapevfl qtgygpacdc wslgvimyem ligyppfcse
      361 npqetyrkvm nwretlvfpp evpiseeakd tiirfccead rrlgaqrgie elklapffrg
      421 vdwehirerp aaipvevrsi ddtsnfdefp dvkleipsap mpqdgeviyk dwvfinytfk
      481 rfegltqrgt ptkk
//
LOCUS       XP_034192790             494 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192790
VERSION     XP_034192790.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336899.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..494
                     /product="serine/threonine-protein kinase tricornered
                     isoform X5"
                     /calculated_mol_wt=56393
     Region          48..112
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(48,51..52,54..56,58..59,62..63,66,69,98,101..103,
                     105..106,109..110)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          115..480
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(123..127,131,144,146,178,194..195,197,201,203,240,
                     242,244..245,247,257..258,261,317..322,349,355,358)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(123..127,131,144,146,178,195..197,201,240,242,
                     244..245,247,257..258)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(127,201,203,240,242,244,261,317..322,349,355,358)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(257..269,313..322)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            474..479
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..494
                     /gene="trc"
                     /coded_by="XM_034336899.2:182..1666"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 mgvaedetps sltshpnpnv nnpnqegvle maatestirf sghtldkatk akvtlenyys
       61 nliaqhierk qrlakleesl kdeglseqqk qekrlqhaqk eteflrlkrs rlgvedfepl
      121 kvigrgafge vrlvqkkdtg hvyamkilrk admlekeqva hvraerdvlv eadhqwvvkm
      181 yysfqdpinl ylimeflpgg dmmtllmkkd tlseectqfy isetalaids ihklgfihrd
      241 ikpdnlllda rghiklsdfg lctglkkshr tdfyrdlsqa kpsdfmtscg sgsggamdsk
      301 rraeswkrnr ralaystvgt pdyiapevfl qtgygpacdc wslgvimyem ligyppfcse
      361 npqetyrkvm nwretlvfpp evpiseeakd tiirfccead rrlgaqrgie elklapffrg
      421 vdwehirerp aaipvevrsi ddtsnfdefp dvkleipsap mpqdgeviyk dwvfinytfk
      481 rfegltqrgt ptkk
//
LOCUS       XP_034192799             464 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192799
VERSION     XP_034192799.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336908.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="serine/threonine-protein kinase tricornered
                     isoform X6"
                     /calculated_mol_wt=53233
     Region          18..82
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(18,21..22,24..26,28..29,32..33,36,39,68,71..73,
                     75..76,79..80)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          85..450
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,164..165,167,171,173,210,212,
                     214..215,217,227..228,231,287..292,319,325,328)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,165..167,171,210,212,
                     214..215,217,227..228)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(97,171,173,210,212,214,231,287..292,319,325,328)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(227..239,283..292)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            444..449
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..464
                     /gene="trc"
                     /coded_by="XM_034336908.2:413..1807"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 maatestirf sghtldkatk akvtlenyys nliaqhierk qrlakleesl kdeglseqqk
       61 qekrlqhaqk eteflrlkrs rlgvedfepl kvigrgafge vrlvqkkdtg hvyamkilrk
      121 admlekeqva hvraerdvlv eadhqwvvkm yysfqdpinl ylimeflpgg dmmtllmkkd
      181 tlseectqfy isetalaids ihklgfihrd ikpdnlllda rghiklsdfg lctglkkshr
      241 tdfyrdlsqa kpsdfmtscg sgsggamdsk rraeswkrnr ralaystvgt pdyiapevfl
      301 qtgygpacdc wslgvimyem ligyppfcse npqetyrkvm nwretlvfpp evpiseeakd
      361 tiirfccead rrlgaqrgie elklapffrg vdwehirerp aaipvevrsi ddtsnfdefp
      421 dvkleipsap mpqdgeviyk dwvfinytfk rfegltqrgt ptkk
//
LOCUS       XP_034192809             464 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192809
VERSION     XP_034192809.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336918.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="serine/threonine-protein kinase tricornered
                     isoform X6"
                     /calculated_mol_wt=53233
     Region          18..82
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(18,21..22,24..26,28..29,32..33,36,39,68,71..73,
                     75..76,79..80)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          85..450
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,164..165,167,171,173,210,212,
                     214..215,217,227..228,231,287..292,319,325,328)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,165..167,171,210,212,
                     214..215,217,227..228)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(97,171,173,210,212,214,231,287..292,319,325,328)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(227..239,283..292)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            444..449
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..464
                     /gene="trc"
                     /coded_by="XM_034336918.2:191..1585"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 maatestirf sghtldkatk akvtlenyys nliaqhierk qrlakleesl kdeglseqqk
       61 qekrlqhaqk eteflrlkrs rlgvedfepl kvigrgafge vrlvqkkdtg hvyamkilrk
      121 admlekeqva hvraerdvlv eadhqwvvkm yysfqdpinl ylimeflpgg dmmtllmkkd
      181 tlseectqfy isetalaids ihklgfihrd ikpdnlllda rghiklsdfg lctglkkshr
      241 tdfyrdlsqa kpsdfmtscg sgsggamdsk rraeswkrnr ralaystvgt pdyiapevfl
      301 qtgygpacdc wslgvimyem ligyppfcse npqetyrkvm nwretlvfpp evpiseeakd
      361 tiirfccead rrlgaqrgie elklapffrg vdwehirerp aaipvevrsi ddtsnfdefp
      421 dvkleipsap mpqdgeviyk dwvfinytfk rfegltqrgt ptkk
//
LOCUS       XP_034192820             464 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192820
VERSION     XP_034192820.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336929.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="serine/threonine-protein kinase tricornered
                     isoform X6"
                     /calculated_mol_wt=53233
     Region          18..82
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(18,21..22,24..26,28..29,32..33,36,39,68,71..73,
                     75..76,79..80)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          85..450
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,164..165,167,171,173,210,212,
                     214..215,217,227..228,231,287..292,319,325,328)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,165..167,171,210,212,
                     214..215,217,227..228)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(97,171,173,210,212,214,231,287..292,319,325,328)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(227..239,283..292)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            444..449
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..464
                     /gene="trc"
                     /coded_by="XM_034336929.2:36..1430"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 maatestirf sghtldkatk akvtlenyys nliaqhierk qrlakleesl kdeglseqqk
       61 qekrlqhaqk eteflrlkrs rlgvedfepl kvigrgafge vrlvqkkdtg hvyamkilrk
      121 admlekeqva hvraerdvlv eadhqwvvkm yysfqdpinl ylimeflpgg dmmtllmkkd
      181 tlseectqfy isetalaids ihklgfihrd ikpdnlllda rghiklsdfg lctglkkshr
      241 tdfyrdlsqa kpsdfmtscg sgsggamdsk rraeswkrnr ralaystvgt pdyiapevfl
      301 qtgygpacdc wslgvimyem ligyppfcse npqetyrkvm nwretlvfpp evpiseeakd
      361 tiirfccead rrlgaqrgie elklapffrg vdwehirerp aaipvevrsi ddtsnfdefp
      421 dvkleipsap mpqdgeviyk dwvfinytfk rfegltqrgt ptkk
//
LOCUS       XP_034192830             464 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine-protein kinase tricornered isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192830
VERSION     XP_034192830.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336939.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..464
                     /product="serine/threonine-protein kinase tricornered
                     isoform X6"
                     /calculated_mol_wt=53233
     Region          18..82
                     /region_name="MobB_Trc-like"
                     /note="Mob-binding domain found in Drosophila melanogaster
                     tricorner and similar proteins; cd21780"
                     /db_xref="CDD:439274"
     Site            order(18,21..22,24..26,28..29,32..33,36,39,68,71..73,
                     75..76,79..80)
                     /site_type="other"
                     /note="putative Mob binding site [polypeptide binding]"
                     /db_xref="CDD:439274"
     Region          85..450
                     /region_name="STKc_NDR_like"
                     /note="Catalytic domain of Nuclear Dbf2-Related
                     kinase-like Protein Serine/Threonine Kinases; cd05599"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,164..165,167,171,173,210,212,
                     214..215,217,227..228,231,287..292,319,325,328)
                     /site_type="active"
                     /db_xref="CDD:270750"
     Site            order(93..97,101,114,116,148,165..167,171,210,212,
                     214..215,217,227..228)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270750"
     Site            order(97,171,173,210,212,214,231,287..292,319,325,328)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270750"
     Site            order(227..239,283..292)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270750"
     Site            444..449
                     /site_type="other"
                     /note="hydrophobic motif (HM)"
                     /db_xref="CDD:270750"
     CDS             1..464
                     /gene="trc"
                     /coded_by="XM_034336939.2:189..1583"
                     /db_xref="GeneID:117609999"
ORIGIN      
        1 maatestirf sghtldkatk akvtlenyys nliaqhierk qrlakleesl kdeglseqqk
       61 qekrlqhaqk eteflrlkrs rlgvedfepl kvigrgafge vrlvqkkdtg hvyamkilrk
      121 admlekeqva hvraerdvlv eadhqwvvkm yysfqdpinl ylimeflpgg dmmtllmkkd
      181 tlseectqfy isetalaids ihklgfihrd ikpdnlllda rghiklsdfg lctglkkshr
      241 tdfyrdlsqa kpsdfmtscg sgsggamdsk rraeswkrnr ralaystvgt pdyiapevfl
      301 qtgygpacdc wslgvimyem ligyppfcse npqetyrkvm nwretlvfpp evpiseeakd
      361 tiirfccead rrlgaqrgie elklapffrg vdwehirerp aaipvevrsi ddtsnfdefp
      421 dvkleipsap mpqdgeviyk dwvfinytfk rfegltqrgt ptkk
//
LOCUS       XP_034192846             577 aa            linear   INV 03-SEP-2025
DEFINITION  nucleostemin 4 [Osmia lignaria lignaria].
ACCESSION   XP_034192846
VERSION     XP_034192846.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336955.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192846.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="nucleostemin 4"
                     /calculated_mol_wt=66783
     Region          161..392
                     /region_name="HSR1_MMR1"
                     /note="A circularly permuted subfamily of the Ras GTPases;
                     cd01857"
                     /db_xref="CDD:206750"
     Site            order(209..210,212..213,240..242,343..349,388)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206750"
     Site            209..212
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206750"
     Site            240..242
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206750"
     Region          <267..>370
                     /region_name="PRK09518"
                     /note="bifunctional cytidylate kinase/GTPase Der;
                     Reviewed"
                     /db_xref="CDD:236546"
     Region          340..520
                     /region_name="Ras_like_GTPase"
                     /note="Rat sarcoma (Ras)-like superfamily of small
                     guanosine triphosphatases (GTPases); cd00882"
                     /db_xref="CDD:206648"
     Site            341..348
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206750"
     Site            341..348
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206648"
     Site            366..373
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206750"
     Site            369
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206750"
     Site            369
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206648"
     Site            385..388
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206750"
     Site            385..388
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206648"
     Site            order(387..388,426..427)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206648"
     Site            387..389
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206750"
     CDS             1..577
                     /gene="Ns4"
                     /coded_by="XM_034336955.2:440..2173"
                     /db_xref="GeneID:117610038"
ORIGIN      
        1 mpqgtrkkpf sgkakkqqlq akkqrqtstl fgagshtrdn eqranygdnl efcsiqkink
       61 qpkddnsskn ryalqffqes keelmkrreq arqtieplsl kdqevsddyf ppeidipkrp
      121 pwdfsmskaq lelreqkyft eyikdmikln sisyfelnle twrqlwrvle msdvlliivd
      181 irytvlmfpp ylyrhitnel kkdmilvlnk vdlappalvv awkeyfstmy pklhilmfts
      241 yptynlrgnv seteglkqrr rkgklrmaae gaqklldtck qivgdnvdlt swqnkiqeem
      301 qmefdlddvs hkdsvtieke dtsyfehery kngiltigci gtpnvgkssl mnalmgkkvv
      361 svsrtpghtk hfqtifltkt vclcdcpglv fpstvpkqlq ilmgcfpiaq vrepyttvkf
      421 laeridvpkl lniqhpendd twsavdvcdg walkrnfvta rtgrldsyra ansilrmale
      481 gkiciyvypp qwvgnkekwe qhpvveivrw iqarnkgedp adtgkvylss edevdeeenl
      541 enekqqkeee sdnsddtsse eseiprvvnk fealsay
//
LOCUS       XP_034192859             519 aa            linear   INV 03-SEP-2025
DEFINITION  putative fatty acyl-CoA reductase CG5065 [Osmia lignaria lignaria].
ACCESSION   XP_034192859
VERSION     XP_034192859.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336968.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192859.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="putative fatty acyl-CoA reductase CG5065"
                     /calculated_mol_wt=59560
     Region          14..334
                     /region_name="FAR-N_SDR_e"
                     /note="fatty acyl CoA reductases (FARs), extended (e)
                     SDRs; cd05236"
                     /db_xref="CDD:187547"
     Site            order(20,22..25,47..49,114..116,133,154..156,207,211,
                     232..235)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Site            order(134,156,207,211)
                     /site_type="active"
                     /db_xref="CDD:187547"
     Site            order(156,207,234,246,262,268)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Region          360..451
                     /region_name="Sterile"
                     /note="Male sterility protein; pfam03015"
                     /db_xref="CDD:460779"
     CDS             1..519
                     /gene="LOC117610041"
                     /coded_by="XM_034336968.2:303..1862"
                     /db_xref="GeneID:117610041"
ORIGIN      
        1 mgelseiqsf ykgktifitg gtglmgkvlv ekllyscsnl nkiyllarak rgnspearit
       61 emfklplfqr irkqkpemmk kviplngdvt tdnlglaneq lkmlinevdi vfhcaatlrl
      121 esklkeaiem ntvgtkrmld lakrlknlka fvylstafcy adkeelderv ydpsedprdi
      181 mrmvqwldes sidqitpkll klhpntytys krlaeklvad eypnlpccia rpsivtpawr
      241 epvagwvdnl ngpvglmvga gkgvirsmhc igtyhaevip vdfainalia ishktatdek
      301 tkdiqaynit qnrimpvtwg eildkgkkia yeypfegqvw ypdgsihsnk fvhdlivfff
      361 hiipaylidf lmlifrqkrf mvrlqnrist glevlqyftt rewifhntnl lvmaaemsrk
      421 dkeifpinfl aideleyikn cilgarqycm kedlstlpka hrhqavmyvv hliavylfyf
      481 gmscliyknf eivricvdsv mdqmkslpil agvvkiihr
//
LOCUS       XP_034192870             478 aa            linear   INV 03-SEP-2025
DEFINITION  protein ABHD18 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034192870
VERSION     XP_034192870.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="protein ABHD18 isoform X1"
                     /calculated_mol_wt=54348
     Region          17..468
                     /region_name="ABHD18"
                     /note="Alpha/beta hydrolase domain containing 18;
                     pfam09752"
                     /db_xref="CDD:370661"
     CDS             1..478
                     /gene="LOC117610051"
                     /coded_by="XM_034336979.2:149..1585"
                     /db_xref="GeneID:117610051"
ORIGIN      
        1 mciwsrldav yrsilltkff tkgwgspenl krifefrkvi anreacynli psdypiniik
       61 deewsdchii egnfetplhk hlpgimpnet itahfqlvlp ckwyshkikp iclhlagtgd
      121 hyfwrrrqlf akpllkesgi gslllenpfy gsrkpenqir sclhnvcdif imggclimes
      181 ivllnwceqq gfgplgltgl smgghmasla atnwpkpipl vpclswstas pvftqgvmsa
      241 sinwtlledq yfanelyqnd lakmvriige edaflagqhf aqhypasmkr iselqdkpqg
      301 aneevatita sptvikketn kdhdsesedn nvkneisedr aarifplnli snrfklkdss
      361 vlkgicllpv prhplfsdck wrerealqfm cgimdecthl knfevpvdte liiavcarnd
      421 ayvprdgcms ldkiwpgaei ryidaghvsa yllhqkvfrs tiieafkrsm kkyplqis
//
LOCUS       XP_034192877             472 aa            linear   INV 03-SEP-2025
DEFINITION  protein ABHD18 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034192877
VERSION     XP_034192877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..472
                     /product="protein ABHD18 isoform X2"
                     /calculated_mol_wt=53751
     Region          17..462
                     /region_name="ABHD18"
                     /note="Alpha/beta hydrolase domain containing 18;
                     pfam09752"
                     /db_xref="CDD:370661"
     CDS             1..472
                     /gene="LOC117610051"
                     /coded_by="XM_034336986.2:149..1567"
                     /db_xref="GeneID:117610051"
ORIGIN      
        1 mciwsrldav yrsilltkff tkgwgspenl krifefrkvi anreacynli psdypiniik
       61 deewsdchii egnfetplhk hlpgimpnet itahfqlvlp ckwyshkikp iclhlagtgd
      121 hyfwrrrqlf akpllkesgi gslllenpfy gsrkpenqir sclhnvcdif imggclimes
      181 ivllnwceqq gfgplgltgl smgghmasla atnwpkpipl vpclswstas pvftqgvmsa
      241 sinwtlledq yfanelyqnd lakmvriige edaflagqhf aqhypasmkr iselqdkpqg
      301 aneevatita sptvikketn kdhdsesedn nvkneisedr aarifplnli snrfklkdss
      361 vlkvprhplf sdckwrerea lqfmcgimde cthlknfevp vdteliiavc arndayvprd
      421 gcmsldkiwp gaeiryidag hvsayllhqk vfrstiieaf krsmkkyplq is
//
LOCUS       XP_034192885             441 aa            linear   INV 03-SEP-2025
DEFINITION  protein ABHD18 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034192885
VERSION     XP_034192885.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034336994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..441
                     /product="protein ABHD18 isoform X3"
                     /calculated_mol_wt=50134
     Region          17..431
                     /region_name="ABHD18"
                     /note="Alpha/beta hydrolase domain containing 18;
                     pfam09752"
                     /db_xref="CDD:370661"
     CDS             1..441
                     /gene="LOC117610051"
                     /coded_by="XM_034336994.2:149..1474"
                     /db_xref="GeneID:117610051"
ORIGIN      
        1 mciwsrldav yrsilltkff tkgwgspenl krifefrkvi anreacynli psdypiniik
       61 deewsdchii egnfetplhk hlpgimpnet itahfqlvlp ckwyshkikp iclhlagtgd
      121 hyfwrrrqlf akpllkesgi gslllenpfy gsrkpenqir sclhnvcdif imggclimes
      181 ivllnwceqq gfgplgltgl smgghmasla atnwpkpipl vpclswstas pvftqgvmsa
      241 sinwtlledq yfanelyqnd lakmvriige edaflagqhf aqhypasmkr iselqdkpqg
      301 aneevatita sptvikketn kdhdsesicl lpvprhplfs dckwrereal qfmcgimdec
      361 thlknfevpv dteliiavca rndayvprdg cmsldkiwpg aeiryidagh vsayllhqkv
      421 frstiieafk rsmkkyplqi s
//
LOCUS       XP_034192892             435 aa            linear   INV 03-SEP-2025
DEFINITION  protein ABHD18 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034192892
VERSION     XP_034192892.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337001.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..435
                     /product="protein ABHD18 isoform X4"
                     /calculated_mol_wt=49540
     Region          17..425
                     /region_name="ABHD18"
                     /note="Alpha/beta hydrolase domain containing 18;
                     pfam09752"
                     /db_xref="CDD:370661"
     CDS             1..435
                     /gene="LOC117610051"
                     /coded_by="XM_034337001.2:149..1456"
                     /db_xref="GeneID:117610051"
ORIGIN      
        1 mciwsrldav yrsilltkff tkgwgspenl krifefrkvi anreacynli psdypiniik
       61 deewsdchii egnfetplhk hlpgimpnet itahfqlvlp ckwyshkikp iclhlagtgd
      121 hyfwrrrqlf akpllkesgi gslllenpfy gsrkpenqir sclhnvcdif imggclimes
      181 ivllnwceqq gfgplgltgl smgghmasla atnwpkpipl vpclswstas pvftqgvmsa
      241 sinwtlledq yfanelyqnd lakmvriige edaflagqhf aqhypasmkr iselqdkpqg
      301 aneevatita sptvikketn kdhdsemprh plfsdckwre realqfmcgi mdecthlknf
      361 evpvdtelii avcarndayv prdgcmsldk iwpgaeiryi daghvsayll hqkvfrstii
      421 eafkrsmkky plqis
//
LOCUS       XP_034192901             393 aa            linear   INV 03-SEP-2025
DEFINITION  protein ABHD18 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034192901
VERSION     XP_034192901.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337010.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="protein ABHD18 isoform X5"
                     /calculated_mol_wt=44326
     Region          1..383
                     /region_name="alpha/beta hydrolases"
                     /note="A functionally diverse superfamily containing
                     proteases, lipases, peroxidases, esterases, epoxide
                     hydrolases and dehalogenases. The catalytic apparatus
                     typically involves three residues (catalytic triad): a
                     serine, a glutamate or aspartate and a...; cl21494"
                     /db_xref="CDD:473884"
     CDS             1..393
                     /gene="LOC117610051"
                     /coded_by="XM_034337010.2:379..1560"
                     /db_xref="GeneID:117610051"
ORIGIN      
        1 mpnetitahf qlvlpckwys hkikpiclhl agtgdhyfwr rrqlfakpll kesgigslll
       61 enpfygsrkp enqirsclhn vcdifimggc limesivlln wceqqgfgpl gltglsmggh
      121 maslaatnwp kpiplvpcls wstaspvftq gvmsasinwt lledqyfane lyqndlakmv
      181 riigeedafl agqhfaqhyp asmkriselq dkpqganeev atitasptvi kketnkdhds
      241 esednnvkne isedraarif plnlisnrfk lkdssvlkgi cllpvprhpl fsdckwrere
      301 alqfmcgimd ecthlknfev pvdteliiav carndayvpr dgcmsldkiw pgaeiryida
      361 ghvsayllhq kvfrstiiea fkrsmkkypl qis
//
LOCUS       XP_034195260             415 aa            linear   INV 03-SEP-2025
DEFINITION  tub domain-containing protein ktub [Osmia lignaria lignaria].
ACCESSION   XP_034195260
VERSION     XP_034195260.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339369.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..415
                     /product="tub domain-containing protein ktub"
                     /calculated_mol_wt=47026
     Region          166..410
                     /region_name="Tub"
                     /note="Tub family; pfam01167"
                     /db_xref="CDD:460094"
     CDS             1..415
                     /gene="ktub"
                     /coded_by="XM_034339369.2:215..1462"
                     /db_xref="GeneID:117611423"
ORIGIN      
        1 mtsldlrqqk leqqrqliaq kmkqkrqsga ggmvqasnis ctqltshstk wmnpnkelrg
       61 ydgplqfsis qtnpdlstfs enkdietrin evsteggfyq tmeatdcade esspidihsp
      121 seslpcsspl rvapsdgasm linrennsss pelegnvegn iehfvlqpan kkmhykcrit
      181 rdrkgmdrgl yptyflhler dygkkiflla grkrkkstts nylistdptd lsrggesyig
      241 klrsnllgtq ftvydngysl ikddkrddrf nprqelaavi ydtnvlgfkg prkmtviipg
      301 mtsdqkrvei cprdesetll erwktknmdn lielhnktpv wnddtqsyvl nfhgrvtqas
      361 vknfqvvhds dvdyvvmqfg rvaedvftmd yrfplctlqa faialssfds klace
//
LOCUS       XP_034192915             413 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquinone biosynthesis protein COQ2, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034192915
VERSION     XP_034192915.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337024.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..413
                     /product="ubiquinone biosynthesis protein COQ2,
                     mitochondrial"
                     /calculated_mol_wt=46016
     Region          101..384
                     /region_name="PT_UbiA"
                     /note="UbiA family of prenyltransferases (PTases);
                     cl00337"
                     /db_xref="CDD:469727"
     CDS             1..413
                     /gene="Coq2"
                     /coded_by="XM_034337024.2:395..1636"
                     /db_xref="GeneID:117610075"
ORIGIN      
        1 mitvwwcqhl frtgkcltsy iqssqsrcvi nqlyscnkih vssftqekqn aenykistqn
       61 vlkpvkllld ekdetthlka anvqsqqkna tfaakiinns ppqirpylkl irmdkpigsw
      121 llfwpcgwsi amaappgalp dlqllalfga gafimrgagc vindmwdqdi dgmvtrtkdr
      181 plvtgeispl qsliflgsql tlgllvllql nlysiilgas slvlvilypv mkrvtywpql
      241 ilgmtfnwga llgwsavygs cdwliclply isgvcwtlly dtiyahqdkm ddiiigikst
      301 alkfgnktkt ylsgfsavmi tglilsgvlv sqtwpyytav glvgthltnq iynlninnpa
      361 dcakrfisnh rigmilfagi vlgnflkdse hkkdasvaqk lrkesttteq qqh
//
LOCUS       XP_034195279             320 aa            linear   INV 03-SEP-2025
DEFINITION  THO complex 3 homolog tex isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034195279
VERSION     XP_034195279.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339388.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="THO complex 3 homolog tex isoform X1"
                     /calculated_mol_wt=35592
     Region          <19..300
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          30..68
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          73..109
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          116..181
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          198..234
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          240..274
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..320
                     /gene="tex"
                     /coded_by="XM_034339388.2:15..977"
                     /db_xref="GeneID:117611437"
ORIGIN      
        1 msasrveeli syfkshnkir eqqshsakvh svgwscdgkl lasgsfdksv cifslgpdrl
       61 kqettfrghg gsvdqlcwha fypellstas gdktvriwdt rtqkctanis trgeninisw
      121 spdgntiavg nkedlvtfid arvmkiraee qfnfevneis wnkdsdtfyl tngqgcvhil
      181 sypdlellhv ikahpgtcic iefdptgryf atgsadalvs lwdadelccl rtfsrlewpv
      241 rtisfsydgq llaaasedlv idigevetge kiadipveaa tftvawhpkq yllayacddk
      301 dtydrkrdag slkvfgfasd
//
LOCUS       XP_034195285             304 aa            linear   INV 03-SEP-2025
DEFINITION  THO complex 3 homolog tex isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034195285
VERSION     XP_034195285.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339394.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="THO complex 3 homolog tex isoform X2"
                     /calculated_mol_wt=33702
     Region          <4..284
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          14..52
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          57..93
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          100..165
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          182..218
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          224..258
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..304
                     /gene="tex"
                     /coded_by="XM_034339394.2:313..1227"
                     /db_xref="GeneID:117611437"
ORIGIN      
        1 misreqqshs akvhsvgwsc dgkllasgsf dksvcifslg pdrlkqettf rghggsvdql
       61 cwhafypell stasgdktvr iwdtrtqkct anistrgeni niswspdgnt iavgnkedlv
      121 tfidarvmki raeeqfnfev neiswnkdsd tfyltngqgc vhilsypdle llhvikahpg
      181 tciciefdpt gryfatgsad alvslwdade lcclrtfsrl ewpvrtisfs ydgqllaaas
      241 edlvidigev etgekiadip veaatftvaw hpkqyllaya cddkdtydrk rdagslkvfg
      301 fasd
//
LOCUS       XP_034192929             293 aa            linear   INV 03-SEP-2025
DEFINITION  protein Abitram [Osmia lignaria lignaria].
ACCESSION   XP_034192929
VERSION     XP_034192929.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337038.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034192929.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="protein Abitram"
                     /calculated_mol_wt=32642
     Site            183
                     /site_type="other"
                     /note="lipoyl-biotinyl attachment site [posttranslational
                     modification]"
                     /db_xref="CDD:133456"
     Region          <194..249
                     /region_name="Biotinyl_lipoyl_domains"
                     /note="Biotinyl_lipoyl_domains are present in
                     biotin-dependent carboxylases/decarboxylases, the
                     dihydrolipoyl acyltransferase component (E2) of 2-oxo acid
                     dehydrogenases, and the H-protein of the glycine cleavage
                     system (GCS). These domains transport CO2, acyl; cl11404"
                     /db_xref="CDD:448245"
     CDS             1..293
                     /gene="LOC117610080"
                     /coded_by="XM_034337038.2:148..1029"
                     /db_xref="GeneID:117610080"
ORIGIN      
        1 mnvlsdnnep vtkrakssdd idkvscnvtd ileennidkn sgcqenanlt dnedegtall
       61 dndeddslfp fppddditdm lqgvkydgsf ptitdryftp yyklnvqclg ddicilmhsn
      121 ricmltlaps hvvfrsdtqi tkidfkvsdk ldrvlnkvsg kskhgaqplq tnsniciitc
      181 sngqtykikc ciigklvevn eallnnpkll lepphkggyl aivlpnikll dkmkeslltq
      241 eqynvkmlec qnaanhlems kictnsllns eseiinndsp mrcsenldtv qkc
//
LOCUS       XP_034192942             286 aa            linear   INV 03-SEP-2025
DEFINITION  heart- and neural crest derivatives-expressed protein [Osmia
            lignaria lignaria].
ACCESSION   XP_034192942
VERSION     XP_034192942.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337051.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="heart- and neural crest derivatives-expressed
                     protein"
                     /calculated_mol_wt=32076
     Region          158..213
                     /region_name="bHLH_TS_HAND"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     heart- and neural crest derivatives-expressed protein
                     (HAND) family; cd11466"
                     /db_xref="CDD:381472"
     Site            order(159,163,165..167,169,174,192..195)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381472"
     Site            order(172..173,176..177,179..180,196,199,203,205..206,210,
                     212..213)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381472"
     CDS             1..286
                     /gene="Hand"
                     /coded_by="XM_034337051.2:318..1178"
                     /db_xref="GeneID:117610087"
ORIGIN      
        1 mlggyetqpd yypqwhqpyn yvtqlpygpl nvpdadsqst ywgadsgisg gssgagspta
       61 stpplieeig vqetsysplq qyshpsinqh ysnlmyppqq elphhlhhhh hhqqqqpqnh
      121 rrdsnystit sinqvdvslq qhlrqgtreg gvvirpkrrn tankkerrrt qsinnafadl
      181 rdcipnvpad tklskiktlr laasyigylm avlesdegee pqtfraeils ngrrnktaqg
      241 nqnesclhaa sslgseessk skgrtgwpqh mwalelkqes ntnqmq
//
LOCUS       XP_034195299             254 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117611450 [Osmia lignaria lignaria].
ACCESSION   XP_034195299
VERSION     XP_034195299.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034339408.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034195299.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..254
                     /product="uncharacterized protein LOC117611450"
                     /calculated_mol_wt=29246
     Region          <163..234
                     /region_name="Hmw_CFAP97"
                     /note="Hemingway/CFA97; pfam13879"
                     /db_xref="CDD:464014"
     CDS             1..254
                     /gene="LOC117611450"
                     /coded_by="XM_034339408.2:1..765"
                     /db_xref="GeneID:117611450"
ORIGIN      
        1 mmsclqnhei ncecqytltf mdnvvektfs eikdsftrvp sihevdeedt deehasehes
       61 etckdvqqit diteavdeds mysnesfcsd escdesegtn vsprslndsh fssqindskc
      121 dkddnedkdk qsenvtqnnd inltcknsic edgsialkqa kskrknmsft ddelrkiewe
      181 nqlllkrima qqrpkdkvfh eniqqprvss sainrrklqr kienenilll qriqqakscv
      241 tntmtkpgyr qtil
//
LOCUS       XP_034192954             252 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192954
VERSION     XP_034192954.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337063.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..252
                     /product="TATA-box binding protein associated factor 11
                     isoform X1"
                     /calculated_mol_wt=28681
     Region          148..233
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(153,155,158..159,161..163,165..166,171,188,192,201,
                     212,214..215,223..225,228..229,231..233,235..237)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(156,159..160,163..164,167..170,173..174,177..178,
                     181..182,197..199,201..203,206,210,221..224,227..228,231)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..252
                     /gene="Taf11"
                     /coded_by="XM_034337063.2:171..929"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 melsklnkfy ytmdnifgke dsgnesehid veekeekgsp sphsgtkdae smdidnmqvk
       61 iesdamssqs qsdveevgsn slctavedql eivkheiemr eeeqatedmf endivlphan
      121 ptsikerres kekskkelee eerekmqvlv snftedqldr yemyrraafp kaaikrimqt
      181 itgcsvsqnv viamsgiakv fvgeiveeal dvmethqetg plqpkhlrea vrrlrlqgqi
      241 pngrahkaff rl
//
LOCUS       XP_034192964             240 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192964
VERSION     XP_034192964.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337073.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="TATA-box binding protein associated factor 11
                     isoform X2"
                     /calculated_mol_wt=27162
     Region          136..221
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(141,143,146..147,149..151,153..154,159,176,180,189,
                     200,202..203,211..213,216..217,219..221,223..225)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(144,147..148,151..152,155..158,161..162,165..166,
                     169..170,185..187,189..191,194,198,209..212,215..216,219)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..240
                     /gene="Taf11"
                     /coded_by="XM_034337073.2:313..1035"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 mdnifgkeds gnesehidve ekeekgspsp hsgtkdaesm didnmqvkie sdamssqsqs
       61 dveevgsnsl ctavedqlei vkheiemree eqatedmfen divlphanpt sikerreske
      121 kskkeleeee rekmqvlvsn ftedqldrye myrraafpka aikrimqtit gcsvsqnvvi
      181 amsgiakvfv geiveealdv methqetgpl qpkhlreavr rlrlqgqipn grahkaffrl
//
LOCUS       XP_034192972             240 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192972
VERSION     XP_034192972.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337081.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="TATA-box binding protein associated factor 11
                     isoform X2"
                     /calculated_mol_wt=27162
     Region          136..221
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(141,143,146..147,149..151,153..154,159,176,180,189,
                     200,202..203,211..213,216..217,219..221,223..225)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(144,147..148,151..152,155..158,161..162,165..166,
                     169..170,185..187,189..191,194,198,209..212,215..216,219)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..240
                     /gene="Taf11"
                     /coded_by="XM_034337081.2:381..1103"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 mdnifgkeds gnesehidve ekeekgspsp hsgtkdaesm didnmqvkie sdamssqsqs
       61 dveevgsnsl ctavedqlei vkheiemree eqatedmfen divlphanpt sikerreske
      121 kskkeleeee rekmqvlvsn ftedqldrye myrraafpka aikrimqtit gcsvsqnvvi
      181 amsgiakvfv geiveealdv methqetgpl qpkhlreavr rlrlqgqipn grahkaffrl
//
LOCUS       XP_034192981             240 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192981
VERSION     XP_034192981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="TATA-box binding protein associated factor 11
                     isoform X2"
                     /calculated_mol_wt=27162
     Region          136..221
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(141,143,146..147,149..151,153..154,159,176,180,189,
                     200,202..203,211..213,216..217,219..221,223..225)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(144,147..148,151..152,155..158,161..162,165..166,
                     169..170,185..187,189..191,194,198,209..212,215..216,219)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..240
                     /gene="Taf11"
                     /coded_by="XM_034337090.2:266..988"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 mdnifgkeds gnesehidve ekeekgspsp hsgtkdaesm didnmqvkie sdamssqsqs
       61 dveevgsnsl ctavedqlei vkheiemree eqatedmfen divlphanpt sikerreske
      121 kskkeleeee rekmqvlvsn ftedqldrye myrraafpka aikrimqtit gcsvsqnvvi
      181 amsgiakvfv geiveealdv methqetgpl qpkhlreavr rlrlqgqipn grahkaffrl
//
LOCUS       XP_034192991             240 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034192991
VERSION     XP_034192991.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337100.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="TATA-box binding protein associated factor 11
                     isoform X2"
                     /calculated_mol_wt=27162
     Region          136..221
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(141,143,146..147,149..151,153..154,159,176,180,189,
                     200,202..203,211..213,216..217,219..221,223..225)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(144,147..148,151..152,155..158,161..162,165..166,
                     169..170,185..187,189..191,194,198,209..212,215..216,219)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..240
                     /gene="Taf11"
                     /coded_by="XM_034337100.2:309..1031"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 mdnifgkeds gnesehidve ekeekgspsp hsgtkdaesm didnmqvkie sdamssqsqs
       61 dveevgsnsl ctavedqlei vkheiemree eqatedmfen divlphanpt sikerreske
      121 kskkeleeee rekmqvlvsn ftedqldrye myrraafpka aikrimqtit gcsvsqnvvi
      181 amsgiakvfv geiveealdv methqetgpl qpkhlreavr rlrlqgqipn grahkaffrl
//
LOCUS       XP_034193002             240 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034193002
VERSION     XP_034193002.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337111.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="TATA-box binding protein associated factor 11
                     isoform X2"
                     /calculated_mol_wt=27162
     Region          136..221
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(141,143,146..147,149..151,153..154,159,176,180,189,
                     200,202..203,211..213,216..217,219..221,223..225)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(144,147..148,151..152,155..158,161..162,165..166,
                     169..170,185..187,189..191,194,198,209..212,215..216,219)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..240
                     /gene="Taf11"
                     /coded_by="XM_034337111.2:209..931"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 mdnifgkeds gnesehidve ekeekgspsp hsgtkdaesm didnmqvkie sdamssqsqs
       61 dveevgsnsl ctavedqlei vkheiemree eqatedmfen divlphanpt sikerreske
      121 kskkeleeee rekmqvlvsn ftedqldrye myrraafpka aikrimqtit gcsvsqnvvi
      181 amsgiakvfv geiveealdv methqetgpl qpkhlreavr rlrlqgqipn grahkaffrl
//
LOCUS       XP_034193010             240 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034193010
VERSION     XP_034193010.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337119.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="TATA-box binding protein associated factor 11
                     isoform X2"
                     /calculated_mol_wt=27162
     Region          136..221
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(141,143,146..147,149..151,153..154,159,176,180,189,
                     200,202..203,211..213,216..217,219..221,223..225)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(144,147..148,151..152,155..158,161..162,165..166,
                     169..170,185..187,189..191,194,198,209..212,215..216,219)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..240
                     /gene="Taf11"
                     /coded_by="XM_034337119.2:161..883"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 mdnifgkeds gnesehidve ekeekgspsp hsgtkdaesm didnmqvkie sdamssqsqs
       61 dveevgsnsl ctavedqlei vkheiemree eqatedmfen divlphanpt sikerreske
      121 kskkeleeee rekmqvlvsn ftedqldrye myrraafpka aikrimqtit gcsvsqnvvi
      181 amsgiakvfv geiveealdv methqetgpl qpkhlreavr rlrlqgqipn grahkaffrl
//
LOCUS       XP_076546858             240 aa            linear   INV 03-SEP-2025
DEFINITION  TATA-box binding protein associated factor 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076546858
VERSION     XP_076546858.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690743.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..240
                     /product="TATA-box binding protein associated factor 11
                     isoform X2"
                     /calculated_mol_wt=27162
     Region          136..221
                     /region_name="TAFII28"
                     /note="hTAFII28-like protein conserved region; pfam04719"
                     /db_xref="CDD:428086"
     Site            order(141,143,146..147,149..151,153..154,159,176,180,189,
                     200,202..203,211..213,216..217,219..221,223..225)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     Site            order(144,147..148,151..152,155..158,161..162,165..166,
                     169..170,185..187,189..191,194,198,209..212,215..216,219)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467028"
     CDS             1..240
                     /gene="Taf11"
                     /coded_by="XM_076690743.1:253..975"
                     /db_xref="GeneID:117610095"
ORIGIN      
        1 mdnifgkeds gnesehidve ekeekgspsp hsgtkdaesm didnmqvkie sdamssqsqs
       61 dveevgsnsl ctavedqlei vkheiemree eqatedmfen divlphanpt sikerreske
      121 kskkeleeee rekmqvlvsn ftedqldrye myrraafpka aikrimqtit gcsvsqnvvi
      181 amsgiakvfv geiveealdv methqetgpl qpkhlreavr rlrlqgqipn grahkaffrl
//
LOCUS       XP_034193023             212 aa            linear   INV 03-SEP-2025
DEFINITION  cytidine/uridine monophosphate kinase Dak1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034193023
VERSION     XP_034193023.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337132.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193023.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..212
                     /product="cytidine/uridine monophosphate kinase Dak1
                     isoform X1"
                     /calculated_mol_wt=23708
     Region          22..208
                     /region_name="UMP_CMP_kin_fam"
                     /note="UMP-CMP kinase family; TIGR01359"
                     /db_xref="CDD:273576"
     CDS             1..212
                     /gene="Dak1"
                     /coded_by="XM_034337132.2:121..759"
                     /db_xref="GeneID:117610123"
ORIGIN      
        1 mfkvfvsglr lftmsaiqkp evlfvlggpg agkgtlcryi tenygyvhls agdllreera
       61 kpgsqygeli enhitngtiv pvaitcslld ramqmsdnvh krflidgfpr nqdnidgwnm
      121 tmsdkciikg vlfcectkev ctqrclkrga agsgrtddne eilvkrhety itntmpiief
      181 fekqgivykv nsmqspkkvf edaknilski gw
//
LOCUS       XP_034193033             199 aa            linear   INV 03-SEP-2025
DEFINITION  cytidine/uridine monophosphate kinase Dak1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034193033
VERSION     XP_034193033.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193033.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="cytidine/uridine monophosphate kinase Dak1
                     isoform X2"
                     /calculated_mol_wt=22181
     Region          9..195
                     /region_name="UMP_CMP_kin_fam"
                     /note="UMP-CMP kinase family; TIGR01359"
                     /db_xref="CDD:273576"
     CDS             1..199
                     /gene="Dak1"
                     /coded_by="XM_034337142.2:172..771"
                     /db_xref="GeneID:117610123"
ORIGIN      
        1 msaiqkpevl fvlggpgagk gtlcryiten ygyvhlsagd llreerakpg sqygelienh
       61 itngtivpva itcslldram qmsdnvhkrf lidgfprnqd nidgwnmtms dkciikgvlf
      121 cectkevctq rclkrgaags grtddneeil vkrhetyitn tmpiieffek qgivykvnsm
      181 qspkkvfeda knilskigw
//
LOCUS       XP_034193045             193 aa            linear   INV 03-SEP-2025
DEFINITION  pheromone biosynthesis-activating neuropeptide [Osmia lignaria
            lignaria].
ACCESSION   XP_034193045
VERSION     XP_034193045.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337154.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..193
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..193
                     /product="pheromone biosynthesis-activating neuropeptide"
                     /calculated_mol_wt=21593
     Region          51..192
                     /region_name="PBAN"
                     /note="Pheromone biosynthesis activating neuropeptide
                     (PBAN); pfam05874"
                     /db_xref="CDD:461765"
     CDS             1..193
                     /gene="Pban"
                     /coded_by="XM_034337154.2:304..885"
                     /db_xref="GeneID:117610129"
ORIGIN      
        1 migsvvisph ratticallc llcvvsstsg eyegrespss vsndrtpgne fgsctdgkci
       61 krttqeissg gmwfgprlgr rrradrklet dpnletlani ldgsrwavit ipggekrqpt
      121 qftprlgres geeffsyayp ndqeelyaee qllpplfapr lgrrvpwtps prlgrqlhsy
      181 leksrqyped srf
//
LOCUS       XP_034193060             155 aa            linear   INV 03-SEP-2025
DEFINITION  biogenesis of lysosome-related organelles complex 1 subunit 4
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193060
VERSION     XP_034193060.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337169.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="biogenesis of lysosome-related organelles
                     complex 1 subunit 4 isoform X1"
                     /calculated_mol_wt=17645
     Region          <22..109
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..155
                     /gene="Blos4"
                     /coded_by="XM_034337169.2:106..573"
                     /db_xref="GeneID:117610134"
ORIGIN      
        1 mtshttpvie elakdyasya kldlsnqmks fhdtiedvmm rleefqsiie mvqsessqcm
       61 eqhiprlqdm qqevinlcrr idalehviam anvnlttlea avdnaeaelg isdrlfgmln
      121 plsffkkaqe pmvpsklpaf epptiyrsdd yfkne
//
LOCUS       XP_034193066             155 aa            linear   INV 03-SEP-2025
DEFINITION  biogenesis of lysosome-related organelles complex 1 subunit 4
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034193066
VERSION     XP_034193066.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337175.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..155
                     /product="biogenesis of lysosome-related organelles
                     complex 1 subunit 4 isoform X1"
                     /calculated_mol_wt=17645
     Region          <22..109
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..155
                     /gene="Blos4"
                     /coded_by="XM_034337175.2:107..574"
                     /db_xref="GeneID:117610134"
ORIGIN      
        1 mtshttpvie elakdyasya kldlsnqmks fhdtiedvmm rleefqsiie mvqsessqcm
       61 eqhiprlqdm qqevinlcrr idalehviam anvnlttlea avdnaeaelg isdrlfgmln
      121 plsffkkaqe pmvpsklpaf epptiyrsdd yfkne
//
LOCUS       XP_076546860             128 aa            linear   INV 03-SEP-2025
DEFINITION  biogenesis of lysosome-related organelles complex 1 subunit 4
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076546860
VERSION     XP_076546860.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076690745.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..128
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..128
                     /product="biogenesis of lysosome-related organelles
                     complex 1 subunit 4 isoform X2"
                     /calculated_mol_wt=14637
     CDS             1..128
                     /gene="Blos4"
                     /coded_by="XM_076690745.1:142..528"
                     /db_xref="GeneID:117610134"
ORIGIN      
        1 mksfhdtied vmmrleefqs iiemvqsess qcmeqhiprl qdmqqevinl crridalehv
       61 iamanvnltt leaavdnaea elgisdrlfg mlnplsffkk aqepmvpskl pafepptiyr
      121 sddyfkne
//
LOCUS       XP_034193090             144 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610150 [Osmia lignaria lignaria].
ACCESSION   XP_034193090
VERSION     XP_034193090.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034337199.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135041) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034193090.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..144
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..144
                     /product="uncharacterized protein LOC117610150"
                     /calculated_mol_wt=16702
     Region          85..131
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     CDS             1..144
                     /gene="LOC117610150"
                     /coded_by="XM_034337199.2:79..513"
                     /db_xref="GeneID:117610150"
ORIGIN      
        1 mlhyfaynik khrylksrel ipgghgkels nlrsyqcpsc deifqnhrrk llsytdeins
       61 hldntglndf lkvtnkdten hgelwdnwsn wskcsvtcgn grqvrwrhcl agdctkglkk
      121 aqmktcrqkq cgsktflhwl giks
//
LOCUS       XP_034190799            1629 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190799
VERSION     XP_034190799.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334908.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190799.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1629
                     /product="uncharacterized protein LOC117609063 isoform X1"
                     /calculated_mol_wt=176519
     Region          47..100
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(52..53,55,60,81..82)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(58..59,62..63,65..66,70,82..83,86..87,90,92..93,
                     96..97)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          129..182
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          282..395
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          522..>658
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1629
                     /gene="LOC117609063"
                     /coded_by="XM_034334908.2:131..5020"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 msiavaenag pspcelqdpl wvkmsaitgs itkkrkksda kpqsqinkcl nekrrrnqen
       61 lfidelaeli svtdmssgkt dkrqilqrtv dqirhirqqe gsnshavqqg evsssnpnil
      121 sndqvgpill ealdgflfvv nsegrveyvt dnitqyinyt kddvlgkdiy niihhgdhnt
      181 fmpslcpvsl gwtsepqpqt rnrtfncrfl vkppddkeet meekqqrvsk yeylqicsal
      241 lpsnsdrles gdvssessdi gpcvmcvarr ippnekpigt pieqftvkld tagkiiavdv
      301 swlsppysny lttvgdligt tikdvchphd lskltahlnd tlqvgestsg vyrlrvspdk
      361 flniqtkskl fkanvmnthd tdfimatnsi igdndlmpie ggqlsnnkvc sghssnrcan
      421 nsnnnngnnn nnvggplmsv ahlngqvsgi sgrglagtss sssasshggg vatssnsivf
      481 saaescnnpl pslstsnpfn hfsgnmdlef elfpsstwdl dsssgwadrp esrasgppns
      541 rppsqpapts pspqgtfssn savaphcspm rafsptsgna ahtfsnsfpf splqesqtss
      601 ltnsgassvs angaaaltpk rqdegktgcs agnnqsamdt sgnarnnssn vagtattagq
      661 qqttvgvtat vvetqnsvvs tesgrlrnll tkgasaseds qdntnndsen qnkhrilktl
      721 lnqqdeddfh pehnnkvrts psnmpkpsmd hsksslgnnm llqllneknd dedeearagf
      781 kkrnellqql lkdqdderkv qeqqcksqnr eedpllrslg frnttpspsq sgdnvglggs
      841 tqvgqkrpge dgdlniavkr pmdgshqvss sgtstnttsk lweknkmlas llakqppqpt
      901 tippipasvi satpqdkllr iglkpqqpsq qsqsqsqqqq qqqqqqqqqq qqqqqqqqqq
      961 qqqpwtggsm qsvggntitt tatsartplq sqsrqlprqt tntylthmls qqqrpqmgqm
     1021 dseftgsgey rhtnadptgw dnqssdpdls dildqviefv pdeaiadssa ianlldaiea
     1081 pqnnalnekm ainaiqkslm lfetavnpts stitipgtpp aysttlvttp vttshsyqpp
     1141 pmyqqqprmr lntqpgvrqt ttqftqqqql qlqqqrtkli qqqqqqqlkq rllqqqqqqq
     1201 llipsnatat dqittgihni dnllnntvap nvslqrssvp dsqvspgygg svqmpsghrl
     1261 ahsyshpstl pqhpivnnnf nsgqqvsaaa rlsphspagi lsfthpqpls prvtqgnygn
     1321 tprlfnvnqv rsqqqptvqq qlqqqqrsmp spgtpasarq spfpaetfpp ptsptasqfp
     1381 pgpnpgapnp saqyrlqrtt stpsattqlp ggvgsprhyg gvskeqplls pshphsgcnp
     1441 atpthnqhnv tntqqhfsnq qhssmiyhtt antintadmq nnqfcydrts vplyssgpgd
     1501 tqdarplppg npvnhqlggn asstsefvrq elraivgart qqqqqqqrvp nniqnnlsgq
     1561 vsqddldalg ltyemspage avvsdgpaks waigsaggap sssrtsmeev argdpkvnqs
     1621 sllqkllse
//
LOCUS       XP_034190800            1625 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190800
VERSION     XP_034190800.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334909.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190800.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1625
                     /product="uncharacterized protein LOC117609063 isoform X2"
                     /calculated_mol_wt=176247
     Region          43..96
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(48..49,51,56,77..78)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(54..55,58..59,61..62,66,78..79,82..83,86,88..89,
                     92..93)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          125..178
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          278..391
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          518..>654
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1625
                     /gene="LOC117609063"
                     /coded_by="XM_034334909.2:835..5712"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 mllgekpspc elqdplwvkm saitgsitkk rkksdakpqs qinkclnekr rrnqenlfid
       61 elaelisvtd mssgktdkrq ilqrtvdqir hirqqegsns havqqgevss snpnilsndq
      121 vgpilleald gflfvvnseg rveyvtdnit qyinytkddv lgkdiyniih hgdhntfmps
      181 lcpvslgwts epqpqtrnrt fncrflvkpp ddkeetmeek qqrvskyeyl qicsallpsn
      241 sdrlesgdvs sessdigpcv mcvarrippn ekpigtpieq ftvkldtagk iiavdvswls
      301 ppysnylttv gdligttikd vchphdlskl tahlndtlqv gestsgvyrl rvspdkflni
      361 qtksklfkan vmnthdtdfi matnsiigdn dlmpieggql snnkvcsghs snrcannsnn
      421 nngnnnnnvg gplmsvahln gqvsgisgrg lagtsssssa sshgggvats snsivfsaae
      481 scnnplpsls tsnpfnhfsg nmdlefelfp sstwdldsss gwadrpesra sgppnsrpps
      541 qpaptspspq gtfssnsava phcspmrafs ptsgnaahtf snsfpfsplq esqtssltns
      601 gassvsanga aaltpkrqde gktgcsagnn qsamdtsgna rnnssnvagt attagqqqtt
      661 vgvtatvvet qnsvvstesg rlrnlltkga sasedsqdnt nndsenqnkh rilktllnqq
      721 deddfhpehn nkvrtspsnm pkpsmdhsks slgnnmllql lneknddede earagfkkrn
      781 ellqqllkdq dderkvqeqq cksqnreedp llrslgfrnt tpspsqsgdn vglggstqvg
      841 qkrpgedgdl niavkrpmdg shqvsssgts tnttsklwek nkmlasllak qppqpttipp
      901 ipasvisatp qdkllriglk pqqpsqqsqs qsqqqqqqqq qqqqqqqqqq qqqqqqqqqp
      961 wtggsmqsvg gntitttats artplqsqsr qlprqttnty lthmlsqqqr pqmgqmdsef
     1021 tgsgeyrhtn adptgwdnqs sdpdlsdild qviefvpdea iadssaianl ldaieapqnn
     1081 alnekmaina iqkslmlfet avnptsstit ipgtppayst tlvttpvtts hsyqpppmyq
     1141 qqprmrlntq pgvrqtttqf tqqqqlqlqq qrtkliqqqq qqqlkqrllq qqqqqqllip
     1201 snatatdqit tgihnidnll nntvapnvsl qrssvpdsqv spgyggsvqm psghrlahsy
     1261 shpstlpqhp ivnnnfnsgq qvsaaarlsp hspagilsft hpqplsprvt qgnygntprl
     1321 fnvnqvrsqq qptvqqqlqq qqrsmpspgt pasarqspfp aetfppptsp tasqfppgpn
     1381 pgapnpsaqy rlqrttstps attqlpggvg sprhyggvsk eqpllspshp hsgcnpatpt
     1441 hnqhnvtntq qhfsnqqhss miyhttanti ntadmqnnqf cydrtsvply ssgpgdtqda
     1501 rplppgnpvn hqlggnasst sefvrqelra ivgartqqqq qqqrvpnniq nnlsgqvsqd
     1561 dldalgltye mspageavvs dgpakswaig saggapsssr tsmeevargd pkvnqssllq
     1621 kllse
//
LOCUS       XP_076548048            1624 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548048
VERSION     XP_076548048.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691933.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1624
                     /product="uncharacterized protein LOC117609063 isoform X3"
                     /calculated_mol_wt=176254
     Region          42..95
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(47..48,50,55,76..77)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(53..54,57..58,60..61,65,77..78,81..82,85,87..88,
                     91..92)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          124..177
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          277..390
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          517..>653
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1624
                     /gene="LOC117609063"
                     /coded_by="XM_076691933.1:16..4890"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 mevyrpspce lqdplwvkms aitgsitkkr kksdakpqsq inkclnekrr rnqenlfide
       61 laelisvtdm ssgktdkrqi lqrtvdqirh irqqegsnsh avqqgevsss npnilsndqv
      121 gpillealdg flfvvnsegr veyvtdnitq yinytkddvl gkdiyniihh gdhntfmpsl
      181 cpvslgwtse pqpqtrnrtf ncrflvkppd dkeetmeekq qrvskyeylq icsallpsns
      241 drlesgdvss essdigpcvm cvarrippne kpigtpieqf tvkldtagki iavdvswlsp
      301 pysnylttvg dligttikdv chphdlsklt ahlndtlqvg estsgvyrlr vspdkflniq
      361 tksklfkanv mnthdtdfim atnsiigdnd lmpieggqls nnkvcsghss nrcannsnnn
      421 ngnnnnnvgg plmsvahlng qvsgisgrgl agtsssssas shgggvatss nsivfsaaes
      481 cnnplpslst snpfnhfsgn mdlefelfps stwdldsssg wadrpesras gppnsrppsq
      541 paptspspqg tfssnsavap hcspmrafsp tsgnaahtfs nsfpfsplqe sqtssltnsg
      601 assvsangaa altpkrqdeg ktgcsagnnq samdtsgnar nnssnvagta ttagqqqttv
      661 gvtatvvetq nsvvstesgr lrnlltkgas asedsqdntn ndsenqnkhr ilktllnqqd
      721 eddfhpehnn kvrtspsnmp kpsmdhskss lgnnmllqll neknddedee aragfkkrne
      781 llqqllkdqd derkvqeqqc ksqnreedpl lrslgfrntt pspsqsgdnv glggstqvgq
      841 krpgedgdln iavkrpmdgs hqvsssgtst nttsklwekn kmlasllakq ppqpttippi
      901 pasvisatpq dkllriglkp qqpsqqsqsq sqqqqqqqqq qqqqqqqqqq qqqqqqqqpw
      961 tggsmqsvgg ntitttatsa rtplqsqsrq lprqttntyl thmlsqqqrp qmgqmdseft
     1021 gsgeyrhtna dptgwdnqss dpdlsdildq viefvpdeai adssaianll daieapqnna
     1081 lnekmainai qkslmlfeta vnptsstiti pgtppaystt lvttpvttsh syqpppmyqq
     1141 qprmrlntqp gvrqtttqft qqqqlqlqqq rtkliqqqqq qqlkqrllqq qqqqqllips
     1201 natatdqitt gihnidnlln ntvapnvslq rssvpdsqvs pgyggsvqmp sghrlahsys
     1261 hpstlpqhpi vnnnfnsgqq vsaaarlsph spagilsfth pqplsprvtq gnygntprlf
     1321 nvnqvrsqqq ptvqqqlqqq qrsmpspgtp asarqspfpa etfppptspt asqfppgpnp
     1381 gapnpsaqyr lqrttstpsa ttqlpggvgs prhyggvske qpllspshph sgcnpatpth
     1441 nqhnvtntqq hfsnqqhssm iyhttantin tadmqnnqfc ydrtsvplys sgpgdtqdar
     1501 plppgnpvnh qlggnassts efvrqelrai vgartqqqqq qqrvpnniqn nlsgqvsqdd
     1561 ldalgltyem spageavvsd gpakswaigs aggapsssrt smeevargdp kvnqssllqk
     1621 llse
//
LOCUS       XP_076548049            1620 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548049
VERSION     XP_076548049.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691934.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1620
                     /product="uncharacterized protein LOC117609063 isoform X4"
                     /calculated_mol_wt=175602
     Region          47..100
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(52..53,55,60,81..82)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(58..59,62..63,65..66,70,82..83,86..87,90,92..93,
                     96..97)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          129..182
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          282..395
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          522..>658
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1620
                     /gene="LOC117609063"
                     /coded_by="XM_076691934.1:132..4994"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 msiavaenag pspcelqdpl wvkmsaitgs itkkrkksda kpqsqinkcl nekrrrnqen
       61 lfidelaeli svtdmssgkt dkrqilqrtv dqirhirqqe gsnshavqqg evsssnpnil
      121 sndqvgpill ealdgflfvv nsegrveyvt dnitqyinyt kddvlgkdiy niihhgdhnt
      181 fmpslcpvsl gwtsepqpqt rnrtfncrfl vkppddkeet meekqqrvsk yeylqicsal
      241 lpsnsdrles gdvssessdi gpcvmcvarr ippnekpigt pieqftvkld tagkiiavdv
      301 swlsppysny lttvgdligt tikdvchphd lskltahlnd tlqvgestsg vyrlrvspdk
      361 flniqtkskl fkanvmnthd tdfimatnsi igdndlmpie ggqlsnnkvc sghssnrcan
      421 nsnnnngnnn nnvggplmsv ahlngqvsgi sgrglagtss sssasshggg vatssnsivf
      481 saaescnnpl pslstsnpfn hfsgnmdlef elfpsstwdl dsssgwadrp esrasgppns
      541 rppsqpapts pspqgtfssn savaphcspm rafsptsgna ahtfsnsfpf splqesqtss
      601 ltnsgassvs angaaaltpk rqdegktgcs agnnqsamdt sgnarnnssn vagtattagq
      661 qqttvgvtat vvetqnsvvs tesgrlrnll tkgasaseds qdntnndsen qnkhrilktl
      721 lnqqdeddfh pehnnkvrts psnmpkpsmd hsksslgnnm llqllneknd dedeearagf
      781 kkrnellqql lkdqdderkv qeqqcksqnr eedpllrslg frnttpspsq sgdnvglggs
      841 tqvgqkrpge dgdlniavkr pmdgshqvss sgtstnttsk lweknkmlas llakqppqpt
      901 tippipasvi satpqdkllr iglkpqqpsq qsqsqsqqqq qqqqqqqqqq qqqqqqqqqq
      961 qqqpwtggsm qsvggntitt tatsartplq sqsrqlprqt tntylthmls qqqrpqmgqm
     1021 dseftgsgey rhtnadptgw dnqssdpdls dildqviefv pdeaiadssa ianlldaiea
     1081 pqnnalnekm ainaiqkslm lfetavnpts stitipgtpp aysttlvttp vttshsyqpp
     1141 pmyqqqprmr lntqpgvrqt ttqftqqqql qlqqqrtkli qqqqqqqlkq rllqqqqqqq
     1201 llipsnatat dqittgihni dnllnntvap nvslqrssvp dsqvspgygg svqmpsghrl
     1261 ahsyshpstl pqhpivnnnf nsgqqvsaaa rlsphspagi lsfthpqpls prvtqgnygn
     1321 tprlfnvnqv rsqqqptvqq qlqqqqrsmp spgtpasarq spfpaetfpp ptsptasqfp
     1381 pgpnpgapnp saqyrlqrtt stpsattqlp ggvgsprhyg gvskeqplls pshphsgcnp
     1441 atpthnqhnv tntqqhfsnq qhssmiyhtt antintadmq nnqfcydrts vplyssgpgd
     1501 tqdarplppg nasstsefvr qelraivgar tqqqqqqqrv pnniqnnlsg qvsqddldal
     1561 gltyemspag eavvsdgpak swaigsagga psssrtsmee vargdpkvnq ssllqkllse
//
LOCUS       XP_076548050            1615 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548050
VERSION     XP_076548050.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691935.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1615
                     /product="uncharacterized protein LOC117609063 isoform X5"
                     /calculated_mol_wt=174943
     Region          47..100
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(52..53,55,60,81..82)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(58..59,62..63,65..66,70,82..83,86..87,90,92..93,
                     96..97)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          129..182
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          282..395
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          522..>658
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1615
                     /gene="LOC117609063"
                     /coded_by="XM_076691935.1:132..4979"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 msiavaenag pspcelqdpl wvkmsaitgs itkkrkksda kpqsqinkcl nekrrrnqen
       61 lfidelaeli svtdmssgkt dkrqilqrtv dqirhirqqe gsnshavqqg evsssnpnil
      121 sndqvgpill ealdgflfvv nsegrveyvt dnitqyinyt kddvlgkdiy niihhgdhnt
      181 fmpslcpvsl gwtsepqpqt rnrtfncrfl vkppddkeet meekqqrvsk yeylqicsal
      241 lpsnsdrles gdvssessdi gpcvmcvarr ippnekpigt pieqftvkld tagkiiavdv
      301 swlsppysny lttvgdligt tikdvchphd lskltahlnd tlqvgestsg vyrlrvspdk
      361 flniqtkskl fkanvmnthd tdfimatnsi igdndlmpie ggqlsnnkvc sghssnrcan
      421 nsnnnngnnn nnvggplmsv ahlngqvsgi sgrglagtss sssasshggg vatssnsivf
      481 saaescnnpl pslstsnpfn hfsgnmdlef elfpsstwdl dsssgwadrp esrasgppns
      541 rppsqpapts pspqgtfssn savaphcspm rafsptsgna ahtfsnsfpf splqesqtss
      601 ltnsgassvs angaaaltpk rqdegktgcs agnnqsamdt sgnarnnssn vagtattagq
      661 qqttvgvtat vvetqnsvvs tesgrlrnll tkgasaseds qdntnndsen qnkhrilktl
      721 lnqqdeddfh pehnnkvrts psnmpkpsmd hsksslgnnm llqllneknd dedeearagf
      781 kkrnellqql lkdqdderkv qeqqcksqnr eedpllrslg frnttpspsq sgdnvglggs
      841 tqvgqkrpge dgdlniavkr pmdgshqvss sgtstnttsk lweknkmlas llakqppqpt
      901 tippipasvi satpqdkllr iglkpqqpsq qsqsqsqqqq qqqqqqqqqq qqqqqqqqqq
      961 qqqpwtggsm qsvggntitt tatsartplq sqsrqlprqt tntylthmls qqqrpqmgqm
     1021 dseftgsgey rhtnadptgw dnqssdpdls dildqviefv pdeaiadssa ianlldaiea
     1081 pqnnalnekm ainaiqkslm lfetavnpts stitipgtpp aysttlvttp vttshsyqpp
     1141 pmyqqqprmr lntqpgvrqt ttqftqqqql qlqqqrtkli qqqqqqqlkq rllqqqqqqq
     1201 llipsnatat dqittgihni dnllnntvap nvslqrssvp dsqvspgygg svqmpsghrl
     1261 ahsyshpstl pqhpivnnnf nsgqqvsaaa rlsphspagi lsfthpqpls prvtqvrsqq
     1321 qptvqqqlqq qqrsmpspgt pasarqspfp aetfppptsp tasqfppgpn pgapnpsaqy
     1381 rlqrttstps attqlpggvg sprhyggvsk eqpllspshp hsgcnpatpt hnqhnvtntq
     1441 qhfsnqqhss miyhttanti ntadmqnnqf cydrtsvply ssgpgdtqda rplppgnpvn
     1501 hqlggnasst sefvrqelra ivgartqqqq qqqrvpnniq nnlsgqvsqd dldalgltye
     1561 mspageavvs dgpakswaig saggapsssr tsmeevargd pkvnqssllq kllse
//
LOCUS       XP_034190803            1606 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190803
VERSION     XP_034190803.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334912.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190803.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1606
                     /product="uncharacterized protein LOC117609063 isoform X6"
                     /calculated_mol_wt=174081
     Region          24..77
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(29..30,32,37,58..59)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(35..36,39..40,42..43,47,59..60,63..64,67,69..70,
                     73..74)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          106..159
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          259..372
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          499..>635
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1606
                     /gene="LOC117609063"
                     /coded_by="XM_034334912.2:406..5226"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 msaitgsitk krkksdakpq sqinkclnek rrrnqenlfi delaelisvt dmssgktdkr
       61 qilqrtvdqi rhirqqegsn shavqqgevs ssnpnilsnd qvgpilleal dgflfvvnse
      121 grveyvtdni tqyinytkdd vlgkdiynii hhgdhntfmp slcpvslgwt sepqpqtrnr
      181 tfncrflvkp pddkeetmee kqqrvskyey lqicsallps nsdrlesgdv ssessdigpc
      241 vmcvarripp nekpigtpie qftvkldtag kiiavdvswl sppysnyltt vgdligttik
      301 dvchphdlsk ltahlndtlq vgestsgvyr lrvspdkfln iqtksklfka nvmnthdtdf
      361 imatnsiigd ndlmpieggq lsnnkvcsgh ssnrcannsn nnngnnnnnv ggplmsvahl
      421 ngqvsgisgr glagtsssss asshgggvat ssnsivfsaa escnnplpsl stsnpfnhfs
      481 gnmdlefelf psstwdldss sgwadrpesr asgppnsrpp sqpaptspsp qgtfssnsav
      541 aphcspmraf sptsgnaaht fsnsfpfspl qesqtssltn sgassvsang aaaltpkrqd
      601 egktgcsagn nqsamdtsgn arnnssnvag tattagqqqt tvgvtatvve tqnsvvstes
      661 grlrnlltkg asasedsqdn tnndsenqnk hrilktllnq qdeddfhpeh nnkvrtspsn
      721 mpkpsmdhsk sslgnnmllq llnekndded eearagfkkr nellqqllkd qdderkvqeq
      781 qcksqnreed pllrslgfrn ttpspsqsgd nvglggstqv gqkrpgedgd lniavkrpmd
      841 gshqvsssgt stnttsklwe knkmlaslla kqppqpttip pipasvisat pqdkllrigl
      901 kpqqpsqqsq sqsqqqqqqq qqqqqqqqqq qqqqqqqqqq pwtggsmqsv ggntitttat
      961 sartplqsqs rqlprqttnt ylthmlsqqq rpqmgqmdse ftgsgeyrht nadptgwdnq
     1021 ssdpdlsdil dqviefvpde aiadssaian lldaieapqn nalnekmain aiqkslmlfe
     1081 tavnptssti tipgtppays ttlvttpvtt shsyqpppmy qqqprmrlnt qpgvrqtttq
     1141 ftqqqqlqlq qqrtkliqqq qqqqlkqrll qqqqqqqlli psnatatdqi ttgihnidnl
     1201 lnntvapnvs lqrssvpdsq vspgyggsvq mpsghrlahs yshpstlpqh pivnnnfnsg
     1261 qqvsaaarls phspagilsf thpqplsprv tqgnygntpr lfnvnqvrsq qqptvqqqlq
     1321 qqqrsmpspg tpasarqspf paetfpppts ptasqfppgp npgapnpsaq yrlqrttstp
     1381 sattqlpggv gsprhyggvs keqpllspsh phsgcnpatp thnqhnvtnt qqhfsnqqhs
     1441 smiyhttant intadmqnnq fcydrtsvpl yssgpgdtqd arplppgnpv nhqlggnass
     1501 tsefvrqelr aivgartqqq qqqqrvpnni qnnlsgqvsq ddldalglty emspageavv
     1561 sdgpakswai gsaggapsss rtsmeevarg dpkvnqssll qkllse
//
LOCUS       XP_076548051            1587 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548051
VERSION     XP_076548051.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691936.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1587
                     /product="uncharacterized protein LOC117609063 isoform X7"
                     /calculated_mol_wt=172320
     Region          47..100
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(52..53,55,60,81..82)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(58..59,62..63,65..66,70,82..83,86..87,90,92..93,
                     96..97)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          129..182
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          282..395
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          522..>658
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1587
                     /gene="LOC117609063"
                     /coded_by="XM_076691936.1:131..4894"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 msiavaenag pspcelqdpl wvkmsaitgs itkkrkksda kpqsqinkcl nekrrrnqen
       61 lfidelaeli svtdmssgkt dkrqilqrtv dqirhirqqe gsnshavqqg evsssnpnil
      121 sndqvgpill ealdgflfvv nsegrveyvt dnitqyinyt kddvlgkdiy niihhgdhnt
      181 fmpslcpvsl gwtsepqpqt rnrtfncrfl vkppddkeet meekqqrvsk yeylqicsal
      241 lpsnsdrles gdvssessdi gpcvmcvarr ippnekpigt pieqftvkld tagkiiavdv
      301 swlsppysny lttvgdligt tikdvchphd lskltahlnd tlqvgestsg vyrlrvspdk
      361 flniqtkskl fkanvmnthd tdfimatnsi igdndlmpie ggqlsnnkvc sghssnrcan
      421 nsnnnngnnn nnvggplmsv ahlngqvsgi sgrglagtss sssasshggg vatssnsivf
      481 saaescnnpl pslstsnpfn hfsgnmdlef elfpsstwdl dsssgwadrp esrasgppns
      541 rppsqpapts pspqgtfssn savaphcspm rafsptsgna ahtfsnsfpf splqesqtss
      601 ltnsgassvs angaaaltpk rqdegktgcs agnnqsamdt sgnarnnssn vagtattagq
      661 qqttvgvtat vvetqnsvvs tesgrlrnll tkgasaseds qdntnndsen qnkhrilktl
      721 lnqqdeddfh pehnnkvrts psnmpkpsmd hsksslgnnm llqllneknd dedeearagf
      781 kkrnellqql lkdqdderkv qeqqcksqnr eedpllrslg frnttpspsq sgdnvglggs
      841 tqvgqkrpge dgdlniavkr pmdgshqvss sgtstnttsk lweknkmlas llakqppqpt
      901 tippipasvi satpqdkllr iglkpqqpsq qsqsqsqqqq qqqqqqqqqq qqqqqqqqqq
      961 qqqpwtggsm qsvggntitt tatsartplq sqsrqlprqt tntylthmls qqqrpqmgqm
     1021 dseftgsgey rhtnadptgw dnqssdpdls dildqviefv pdeaiadssa ianlldaiea
     1081 pqnnalnekm ainaiqkslm lfetavnpts stitipgtpp aysttlvttp vttshsyqpp
     1141 pmyqqqprmr lntqpgvrqt ttqftqqqql qlqqqrtkli qqqqqqqlkq rllqqqqqqq
     1201 llipsnatat dqittgihni dnllnntvap nvslqrssvp dsqvspgygg svqmpsghrl
     1261 ahsyshpstl pqhpivnnnf nsgqqvsaaa rlsphspagi lsfthpqpls prvtqgnygn
     1321 tprlfnvnqv rsqqqptvqq qlqqqqrsmp spgtpasarq spfpaetfpp ptsptasqfp
     1381 pgpnpgapnp saqyrlqrtt stpsattqlp ggvgsprhyg gvskeqplls pshphsgcnp
     1441 atpthnqhnv tntqqhfsnq qhssmiyhtt antintadmq nnqfcydrts vplyssgpgd
     1501 tqdarplppg npvnhqlggn asstsefvrq elraivgart qqqqqqqrvp nniqnnlsgq
     1561 vsqddldalg ltyemspadf yggsgsr
//
LOCUS       XP_076548052            1521 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548052
VERSION     XP_076548052.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691937.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1521
                     /product="uncharacterized protein LOC117609063 isoform X8"
                     /calculated_mol_wt=164869
     Region          47..100
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(52..53,55,60,81..82)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(58..59,62..63,65..66,70,82..83,86..87,90,92..93,
                     96..97)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          129..182
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          282..395
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     Region          522..>658
                     /region_name="DUF5585"
                     /note="Family of unknown function (DUF5585); pfam17823"
                     /db_xref="CDD:465521"
     CDS             1..1521
                     /gene="LOC117609063"
                     /coded_by="XM_076691937.1:132..4697"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 msiavaenag pspcelqdpl wvkmsaitgs itkkrkksda kpqsqinkcl nekrrrnqen
       61 lfidelaeli svtdmssgkt dkrqilqrtv dqirhirqqe gsnshavqqg evsssnpnil
      121 sndqvgpill ealdgflfvv nsegrveyvt dnitqyinyt kddvlgkdiy niihhgdhnt
      181 fmpslcpvsl gwtsepqpqt rnrtfncrfl vkppddkeet meekqqrvsk yeylqicsal
      241 lpsnsdrles gdvssessdi gpcvmcvarr ippnekpigt pieqftvkld tagkiiavdv
      301 swlsppysny lttvgdligt tikdvchphd lskltahlnd tlqvgestsg vyrlrvspdk
      361 flniqtkskl fkanvmnthd tdfimatnsi igdndlmpie ggqlsnnkvc sghssnrcan
      421 nsnnnngnnn nnvggplmsv ahlngqvsgi sgrglagtss sssasshggg vatssnsivf
      481 saaescnnpl pslstsnpfn hfsgnmdlef elfpsstwdl dsssgwadrp esrasgppns
      541 rppsqpapts pspqgtfssn savaphcspm rafsptsgna ahtfsnsfpf splqesqtss
      601 ltnsgassvs angaaaltpk rqdegktgcs agnnqsamdt sgnarnnssn vagtattagq
      661 qqttvgvtat vvetqnsvvs tesgrlrnll tkgasaseds qdntnndsen qnkhrilktl
      721 lnqqdeddfh pehnnkvrts psnmpkpsmd hsksslgnnm llqllneknd dedeearagf
      781 kkrnellqql lkdqdderkv qeqqcksqnr eedpllrslg frnttpspsq sgdnvglggs
      841 tqvgqkrpge dgdlniavkr pmdgshqvss sgtstnttsk lweknkmlas llakqppqpt
      901 tippipasvi satpqdkllr iglkpqqpsq qsqsqsqqqq qqqqqqqqqq qqqqqqqqqq
      961 qqqpwtggsm qsvggntitt tatsartplq sqsrqlprqt tntylthmls qqqrpqmgqm
     1021 dseftgsgey rhtnadptgw dnqssdpdls dildqviefv pdeaiadssa ianlldaiea
     1081 pqnnalnekm ainaiqkslm lfetavnpts stitipgtpp aysttlvttp vttshsyqpp
     1141 pmyqqqprmr lntqpgvrqt ttqftqqqql qlqqqrtkli qqqqqqqlkq rllqqqqqqq
     1201 llipsnatat dqittgihni dnllnntvap nvslqrssvp dsqvspgygg svqmpsghrl
     1261 ahsyshpstl pqhpivnnnf nsgqqvsaaa rlsphspagi lsfthpqpls prvtqgnygn
     1321 tprlfnvnqv rsqqqptvqq qlqqqqrsmp spgtpasarq spfpaetfpp ptsptasqfp
     1381 pgpnpgapnp saqyrlqrtt stpsattqlp gnasstsefv rqelraivga rtqqqqqqqr
     1441 vpnniqnnls gqvsqddlda lgltyemspa geavvsdgpa kswaigsagg apsssrtsme
     1501 evargdpkvn qssllqklls e
//
LOCUS       XP_076548053            1430 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548053
VERSION     XP_076548053.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691938.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1430
                     /product="uncharacterized protein LOC117609063 isoform X9"
                     /calculated_mol_wt=156233
     Region          47..100
                     /region_name="bHLH_SF"
                     /note="basic Helix Loop Helix (bHLH) domain superfamily;
                     cl00081"
                     /db_xref="CDD:469605"
     Site            order(52..53,55,60,81..82)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381445"
     Site            order(58..59,62..63,65..66,70,82..83,86..87,90,92..93,
                     96..97)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381445"
     Region          129..182
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          282..392
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     CDS             1..1430
                     /gene="LOC117609063"
                     /coded_by="XM_076691938.1:131..4423"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 msiavaenag pspcelqdpl wvkmsaitgs itkkrkksda kpqsqinkcl nekrrrnqen
       61 lfidelaeli svtdmssgkt dkrqilqrtv dqirhirqqe gsnshavqqg evsssnpnil
      121 sndqvgpill ealdgflfvv nsegrveyvt dnitqyinyt kddvlgkdiy niihhgdhnt
      181 fmpslcpvsl gwtsepqpqt rnrtfncrfl vkppddkeet meekqqrvsk yeylqicsal
      241 lpsnsdrles gdvssessdi gpcvmcvarr ippnekpigt pieqftvkld tagkiiavdv
      301 swlsppysny lttvgdligt tikdvchphd lskltahlnd tlqvgestsg vyrlrvspdk
      361 flniqtkskl fkanvmnthd tdfimatnsi igplqesqts sltnsgassv sangaaaltp
      421 krqdegktgc sagnnqsamd tsgnarnnss nvagtattag qqqttvgvta tvvetqnsvv
      481 stesgrlrnl ltkgasased sqdntnndse nqnkhrilkt llnqqdeddf hpehnnkvrt
      541 spsnmpkpsm dhsksslgnn mllqllnekn ddedeearag fkkrnellqq llkdqdderk
      601 vqeqqcksqn reedpllrsl gfrnttpsps qsgdnvglgg stqvgqkrpg edgdlniavk
      661 rpmdgshqvs ssgtstntts klweknkmla sllakqppqp ttippipasv isatpqdkll
      721 riglkpqqps qqsqsqsqqq qqqqqqqqqq qqqqqqqqqq qqqqpwtggs mqsvggntit
      781 ttatsartpl qsqsrqlprq ttntylthml sqqqrpqmgq mdseftgsge yrhtnadptg
      841 wdnqssdpdl sdildqvief vpdeaiadss aianlldaie apqnnalnek mainaiqksl
      901 mlfetavnpt sstitipgtp paysttlvtt pvttshsyqp ppmyqqqprm rlntqpgvrq
      961 tttqftqqqq lqlqqqrtkl iqqqqqqqlk qrllqqqqqq qllipsnata tdqittgihn
     1021 idnllnntva pnvslqrssv pdsqvspgyg gsvqmpsghr lahsyshpst lpqhpivnnn
     1081 fnsgqqvsaa arlsphspag ilsfthpqpl sprvtqgnyg ntprlfnvnq vrsqqqptvq
     1141 qqlqqqqrsm pspgtpasar qspfpaetfp pptsptasqf ppgpnpgapn psaqyrlqrt
     1201 tstpsattql pggvgsprhy ggvskeqpll spshphsgcn patpthnqhn vtntqqhfsn
     1261 qqhssmiyht tantintadm qnnqfcydrt svplyssgpg dtqdarplpp gnpvnhqlgg
     1321 nasstsefvr qelraivgar tqqqqqqqrv pnniqnnlsg qvsqddldal gltyemspag
     1381 eavvsdgpak swaigsagga psssrtsmee vargdpkvnq ssllqkllse
//
LOCUS       XP_034190807            1409 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609063 isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190807
VERSION     XP_034190807.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334916.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190807.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1409
                     /product="uncharacterized protein LOC117609063 isoform
                     X10"
                     /calculated_mol_wt=151698
     Region          62..175
                     /region_name="PAS_11"
                     /note="PAS domain; pfam14598"
                     /db_xref="CDD:464214"
     CDS             1..1409
                     /gene="LOC117609063"
                     /coded_by="XM_034334916.2:207..4436"
                     /db_xref="GeneID:117609063"
ORIGIN      
        1 meekqqrvsk yeylqicsal lpsnsdrles gdvssessdi gpcvmcvarr ippnekpigt
       61 pieqftvkld tagkiiavdv swlsppysny lttvgdligt tikdvchphd lskltahlnd
      121 tlqvgestsg vyrlrvspdk flniqtkskl fkanvmnthd tdfimatnsi igdndlmpie
      181 ggqlsnnkvc sghssnrcan nsnnnngnnn nnvggplmsv ahlngqvsgi sgrglagtss
      241 sssasshggg vatssnsivf saaescnnpl pslstsnpfn hfsgnmdlef elfpsstwdl
      301 dsssgwadrp esrasgppns rppsqpapts pspqgtfssn savaphcspm rafsptsgna
      361 ahtfsnsfpf splqesqtss ltnsgassvs angaaaltpk rqdegktgcs agnnqsamdt
      421 sgnarnnssn vagtattagq qqttvgvtat vvetqnsvvs tesgrlrnll tkgasaseds
      481 qdntnndsen qnkhrilktl lnqqdeddfh pehnnkvrts psnmpkpsmd hsksslgnnm
      541 llqllneknd dedeearagf kkrnellqql lkdqdderkv qeqqcksqnr eedpllrslg
      601 frnttpspsq sgdnvglggs tqvgqkrpge dgdlniavkr pmdgshqvss sgtstnttsk
      661 lweknkmlas llakqppqpt tippipasvi satpqdkllr iglkpqqpsq qsqsqsqqqq
      721 qqqqqqqqqq qqqqqqqqqq qqqpwtggsm qsvggntitt tatsartplq sqsrqlprqt
      781 tntylthmls qqqrpqmgqm dseftgsgey rhtnadptgw dnqssdpdls dildqviefv
      841 pdeaiadssa ianlldaiea pqnnalnekm ainaiqkslm lfetavnpts stitipgtpp
      901 aysttlvttp vttshsyqpp pmyqqqprmr lntqpgvrqt ttqftqqqql qlqqqrtkli
      961 qqqqqqqlkq rllqqqqqqq llipsnatat dqittgihni dnllnntvap nvslqrssvp
     1021 dsqvspgygg svqmpsghrl ahsyshpstl pqhpivnnnf nsgqqvsaaa rlsphspagi
     1081 lsfthpqpls prvtqgnygn tprlfnvnqv rsqqqptvqq qlqqqqrsmp spgtpasarq
     1141 spfpaetfpp ptsptasqfp pgpnpgapnp saqyrlqrtt stpsattqlp ggvgsprhyg
     1201 gvskeqplls pshphsgcnp atpthnqhnv tntqqhfsnq qhssmiyhtt antintadmq
     1261 nnqfcydrts vplyssgpgd tqdarplppg npvnhqlggn asstsefvrq elraivgart
     1321 qqqqqqqrvp nniqnnlsgq vsqddldalg ltyemspage avvsdgpaks waigsaggap
     1381 sssrtsmeev argdpkvnqs sllqkllse
//
LOCUS       XP_034190848            1073 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190848
VERSION     XP_034190848.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334957.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1073
                     /product="inactivation no afterpotential E isoform X1"
                     /calculated_mol_wt=118989
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1073
                     /gene="inaE"
                     /coded_by="XM_034334957.2:214..3435"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpaliafgrr wrvgsddlff pgatlaciha levtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkgeq kyerrwrrvl hkqepvyqal
      661 wagpcdfdev lispvmiqdh mpdnmlkaln kvvttlgpak pqrlasghas steasearev
      721 veiqemeieq emrallssss pvkshpsnla gtpphrlcle tsftslqsps efpqvgrels
      781 vdsrgipwey vslasellnt pradeskssn lrsnwddasr maplatpetl seasssppsp
      841 vppprpmrrt pkisgnlsta addlknnlhf amlsaknyfl regtngsnts sgnseasyes
      901 aksltaglpp pvprrrdsvi irreqrvcta accrddlsen ssshtsshss rtshasnqvq
      961 pgfaeeenet ngssldffea kgssgqrdss ndvflsvrss peckglmapa tdlgaewkkk
     1021 fdasgmsgda slpllrglsp tpthgqhssp ffnakrkkyv ypitlvgrge ssv
//
LOCUS       XP_034190849            1073 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190849
VERSION     XP_034190849.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334958.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1073
                     /product="inactivation no afterpotential E isoform X2"
                     /calculated_mol_wt=119184
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1073
                     /gene="inaE"
                     /coded_by="XM_034334958.2:807..4028"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpgivvfrrr wsvgsddlvv pgtflfilhl iwvtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkgeq kyerrwrrvl hkqepvyqal
      661 wagpcdfdev lispvmiqdh mpdnmlkaln kvvttlgpak pqrlasghas steasearev
      721 veiqemeieq emrallssss pvkshpsnla gtpphrlcle tsftslqsps efpqvgrels
      781 vdsrgipwey vslasellnt pradeskssn lrsnwddasr maplatpetl seasssppsp
      841 vppprpmrrt pkisgnlsta addlknnlhf amlsaknyfl regtngsnts sgnseasyes
      901 aksltaglpp pvprrrdsvi irreqrvcta accrddlsen ssshtsshss rtshasnqvq
      961 pgfaeeenet ngssldffea kgssgqrdss ndvflsvrss peckglmapa tdlgaewkkk
     1021 fdasgmsgda slpllrglsp tpthgqhssp ffnakrkkyv ypitlvgrge ssv
//
LOCUS       XP_034190851            1073 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190851
VERSION     XP_034190851.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334960.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1073
                     /product="inactivation no afterpotential E isoform X2"
                     /calculated_mol_wt=119184
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1073
                     /gene="inaE"
                     /coded_by="XM_034334960.2:822..4043"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpgivvfrrr wsvgsddlvv pgtflfilhl iwvtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkgeq kyerrwrrvl hkqepvyqal
      661 wagpcdfdev lispvmiqdh mpdnmlkaln kvvttlgpak pqrlasghas steasearev
      721 veiqemeieq emrallssss pvkshpsnla gtpphrlcle tsftslqsps efpqvgrels
      781 vdsrgipwey vslasellnt pradeskssn lrsnwddasr maplatpetl seasssppsp
      841 vppprpmrrt pkisgnlsta addlknnlhf amlsaknyfl regtngsnts sgnseasyes
      901 aksltaglpp pvprrrdsvi irreqrvcta accrddlsen ssshtsshss rtshasnqvq
      961 pgfaeeenet ngssldffea kgssgqrdss ndvflsvrss peckglmapa tdlgaewkkk
     1021 fdasgmsgda slpllrglsp tpthgqhssp ffnakrkkyv ypitlvgrge ssv
//
LOCUS       XP_034190852            1073 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190852
VERSION     XP_034190852.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334961.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1073
                     /product="inactivation no afterpotential E isoform X2"
                     /calculated_mol_wt=119184
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1073
                     /gene="inaE"
                     /coded_by="XM_034334961.2:808..4029"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpgivvfrrr wsvgsddlvv pgtflfilhl iwvtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkgeq kyerrwrrvl hkqepvyqal
      661 wagpcdfdev lispvmiqdh mpdnmlkaln kvvttlgpak pqrlasghas steasearev
      721 veiqemeieq emrallssss pvkshpsnla gtpphrlcle tsftslqsps efpqvgrels
      781 vdsrgipwey vslasellnt pradeskssn lrsnwddasr maplatpetl seasssppsp
      841 vppprpmrrt pkisgnlsta addlknnlhf amlsaknyfl regtngsnts sgnseasyes
      901 aksltaglpp pvprrrdsvi irreqrvcta accrddlsen ssshtsshss rtshasnqvq
      961 pgfaeeenet ngssldffea kgssgqrdss ndvflsvrss peckglmapa tdlgaewkkk
     1021 fdasgmsgda slpllrglsp tpthgqhssp ffnakrkkyv ypitlvgrge ssv
//
LOCUS       XP_034190853            1073 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190853
VERSION     XP_034190853.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334962.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1073
                     /product="inactivation no afterpotential E isoform X2"
                     /calculated_mol_wt=119184
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1073
                     /gene="inaE"
                     /coded_by="XM_034334962.2:810..4031"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpgivvfrrr wsvgsddlvv pgtflfilhl iwvtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkgeq kyerrwrrvl hkqepvyqal
      661 wagpcdfdev lispvmiqdh mpdnmlkaln kvvttlgpak pqrlasghas steasearev
      721 veiqemeieq emrallssss pvkshpsnla gtpphrlcle tsftslqsps efpqvgrels
      781 vdsrgipwey vslasellnt pradeskssn lrsnwddasr maplatpetl seasssppsp
      841 vppprpmrrt pkisgnlsta addlknnlhf amlsaknyfl regtngsnts sgnseasyes
      901 aksltaglpp pvprrrdsvi irreqrvcta accrddlsen ssshtsshss rtshasnqvq
      961 pgfaeeenet ngssldffea kgssgqrdss ndvflsvrss peckglmapa tdlgaewkkk
     1021 fdasgmsgda slpllrglsp tpthgqhssp ffnakrkkyv ypitlvgrge ssv
//
LOCUS       XP_034190854            1073 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190854
VERSION     XP_034190854.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334963.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1073
                     /product="inactivation no afterpotential E isoform X2"
                     /calculated_mol_wt=119184
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1073
                     /gene="inaE"
                     /coded_by="XM_034334963.2:802..4023"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpgivvfrrr wsvgsddlvv pgtflfilhl iwvtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkgeq kyerrwrrvl hkqepvyqal
      661 wagpcdfdev lispvmiqdh mpdnmlkaln kvvttlgpak pqrlasghas steasearev
      721 veiqemeieq emrallssss pvkshpsnla gtpphrlcle tsftslqsps efpqvgrels
      781 vdsrgipwey vslasellnt pradeskssn lrsnwddasr maplatpetl seasssppsp
      841 vppprpmrrt pkisgnlsta addlknnlhf amlsaknyfl regtngsnts sgnseasyes
      901 aksltaglpp pvprrrdsvi irreqrvcta accrddlsen ssshtsshss rtshasnqvq
      961 pgfaeeenet ngssldffea kgssgqrdss ndvflsvrss peckglmapa tdlgaewkkk
     1021 fdasgmsgda slpllrglsp tpthgqhssp ffnakrkkyv ypitlvgrge ssv
//
LOCUS       XP_034190855            1065 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190855
VERSION     XP_034190855.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334964.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1065
                     /product="inactivation no afterpotential E isoform X3"
                     /calculated_mol_wt=117785
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1065
                     /gene="inaE"
                     /coded_by="XM_034334964.2:213..3410"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpaliafgrr wrvgsddlff pgatlaciha levtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkger vlhkqepvyq alwagpcdfd
      661 evlispvmiq dhmpdnmlka lnkvvttlgp akpqrlasgh assteasear evveiqemei
      721 eqemrallss sspvkshpsn lagtpphrlc letsftslqs psefpqvgre lsvdsrgipw
      781 eyvslasell ntpradesks snlrsnwdda srmaplatpe tlseassspp spvppprpmr
      841 rtpkisgnls taaddlknnl hfamlsakny flregtngsn tssgnseasy esaksltagl
      901 pppvprrrds viirreqrvc taaccrddls enssshtssh ssrtshasnq vqpgfaeeen
      961 etngssldff eakgssgqrd ssndvflsvr sspeckglma patdlgaewk kkfdasgmsg
     1021 daslpllrgl sptpthgqhs spffnakrkk yvypitlvgr gessv
//
LOCUS       XP_034190857            1021 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190857
VERSION     XP_034190857.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334966.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1021
                     /product="inactivation no afterpotential E isoform X4"
                     /calculated_mol_wt=113416
     Region          <388..546
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(410..411,413..421)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            480..484
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1021
                     /gene="inaE"
                     /coded_by="XM_034334966.2:213..3278"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mpaliafgrr wrvgsddlff pgatlaciha levtvlcvll gilewdrsvt cilllweyiv
       61 gylaifvism mvefsicfla trgsildtaa rapmqyilyv rlflmlietg wlcagvtwlv
      121 ryyqtcpvdr mndvtlglvi snwcvlasvi vtvwctydaa grswvkmkky qrsmreaesr
      181 cgklhynrsg srnrnwrqrk virayqdswd nrcrllfccm gnsdrsrnsf tdiarllsdf
      241 frdldvvpsd vvaglvllrk fqkvereliv kqrkndtyef lsgvpvtprt kflsltedgd
      301 lghfqlaihy mhfalaaygw pmflvshstg lcqlctrlrc gcfpcgghqn eativedncc
      361 kcnyaalkkm velaeveviy atfhvdvget pffvaldytk kkvvvsirgt lsmkdvltdl
      421 naegevlpls pprddwlghk gmvqaaeyir kklleeeiis rafakdpsrg thqfgltlvg
      481 hslgagtaai laillkqdyp dlvcfsfapp ggllsmpaqq ytqefitsvv vgkdvvprig
      541 lrqmeslrad linaikrsvd pkwktiacsv mccgcgstpt saanleaggc iseyqrdkdl
      601 arsqtvvpsd ssialtlhrp lyppgriihv vrhhpnkgeq kyerrwrrvl hkqepvyqal
      661 wagpcdfdev lispvmiqdh mpdnmlkaln kvsacrdrdp geadaathet iatdattqgr
      721 pdqqhvvlhr qstptslhdh dadvyatths srhgtrsnlr snwddasrma platpetlse
      781 asssppspvp pprpmrrtpk isgnlstaad dlknnlhfam lsaknyflre gtngsntssg
      841 nseasyesak sltaglpppv prrrdsviir reqrvctaac crddlsenss shtsshssrt
      901 shasnqvqpg faeeenetng ssldffeakg ssgqrdssnd vflsvrsspe ckglmapatd
      961 lgaewkkkfd asgmsgdasl pllrglsptp thgqhsspff nakrkkyvyp itlvgrgess
     1021 v
//
LOCUS       XP_034190858            1004 aa            linear   INV 03-SEP-2025
DEFINITION  inactivation no afterpotential E isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190858
VERSION     XP_034190858.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334967.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1004
                     /product="inactivation no afterpotential E isoform X5"
                     /calculated_mol_wt=111266
     Region          <319..477
                     /region_name="Lipase_3"
                     /note="Lipase (class 3). Lipases are esterases that can
                     hydrolyze long-chain acyl-triglycerides into di- and
                     monoglycerides, glycerol, and free fatty acids at a
                     water/lipid interface. A typical feature of lipases is
                     'interfacial activation,' the process of...; cd00519"
                     /db_xref="CDD:238287"
     Site            order(341..342,344..352)
                     /site_type="active"
                     /note="active site flap/lid [active]"
                     /db_xref="CDD:238287"
     Site            411..415
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238287"
     CDS             1..1004
                     /gene="inaE"
                     /coded_by="XM_034334967.2:436..3450"
                     /db_xref="GeneID:117609082"
ORIGIN      
        1 mmvefsicfl atrgsildta arapmqyily vrlflmliet gwlcagvtwl vryyqtcpvd
       61 rmndvtlglv isnwcvlasv ivtvwctyda agrswvkmkk yqrsmreaes rcgklhynrs
      121 gsrnrnwrqr kvirayqdsw dnrcrllfcc mgnsdrsrns ftdiarllsd ffrdldvvps
      181 dvvaglvllr kfqkvereli vkqrkndtye flsgvpvtpr tkflsltedg dlghfqlaih
      241 ymhfalaayg wpmflvshst glcqlctrlr cgcfpcgghq neativednc ckcnyaalkk
      301 mvelaevevi yatfhvdvge tpffvaldyt kkkvvvsirg tlsmkdvltd lnaegevlpl
      361 spprddwlgh kgmvqaaeyi rkklleeeii srafakdpsr gthqfgltlv ghslgagtaa
      421 ilaillkqdy pdlvcfsfap pggllsmpaq qytqefitsv vvgkdvvpri glrqmeslra
      481 dlinaikrsv dpkwktiacs vmccgcgstp tsaanleagg ciseyqrdkd larsqtvvps
      541 dssialtlhr plyppgriih vvrhhpnkge qkyerrwrrv lhkqepvyqa lwagpcdfde
      601 vlispvmiqd hmpdnmlkal nkvvttlgpa kpqrlasgha ssteaseare vveiqemeie
      661 qemrallsss spvkshpsnl agtpphrlcl etsftslqsp sefpqvgrel svdsrgipwe
      721 yvslaselln tpradeskss nlrsnwddas rmaplatpet lseassspps pvppprpmrr
      781 tpkisgnlst aaddlknnlh famlsaknyf lregtngsnt ssgnseasye saksltaglp
      841 ppvprrrdsv iirreqrvct aaccrddlse nssshtsshs srtshasnqv qpgfaeeene
      901 tngssldffe akgssgqrds sndvflsvrs speckglmap atdlgaewkk kfdasgmsgd
      961 aslpllrgls ptpthgqhss pffnakrkky vypitlvgrg essv
//
LOCUS       XP_034190865             856 aa            linear   INV 03-SEP-2025
DEFINITION  protein lin-54 homolog isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190865
VERSION     XP_034190865.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334974.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..856
                     /product="protein lin-54 homolog isoform X1"
                     /calculated_mol_wt=92553
     Region          <133..486
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          636..>660
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          711..748
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..856
                     /gene="LOC117609088"
                     /coded_by="XM_034334974.2:35..2605"
                     /db_xref="GeneID:117609088"
ORIGIN      
        1 mlmfekyirk fyeyyvviki ssvalphpsl niimsvnkgq naralvepla ldsrtfgdsd
       61 lsalslshnn eqyssndfea faniqaelec inaeevmstd eehiiigrni etetivpdve
      121 mteiseqvee ehiiyttanq nnqnivfqtk ptlqrlptst vqvksnagqv tqsqsimivs
      181 paggqgasqi lkishpstas agqlqslaqt litaksadgn ivqlrsaqpn kplmatshsg
      241 sitlgnvhns ktaqsaikrt aqstsqnrnv ytkmilagnq aqsgqvlitn sqnenqqaik
      301 flnnntssqe vtnptktitl aqaqqmglls tskvqhilps tpqkqgiivn klvqssssqp
      361 skmtivssnt iksptkilpa pvintqvkts slsnqqstfs cnkssvqqsp qkviirqssl
      421 kpgtvlgsgq viripasqni vtgsnqvhqi qmpgrqvqyv rlvstpssgt tnvvtvgktk
      481 sqttlqtvgv gqkiggqqiv kvvplntsnq slrtvapkat lsgsgqrlli patatvgnqs
      541 knavaipasa lsqlasgqav istnsnvgni vvlpaqyiqq stdevkikpq piapsllgts
      601 qnlqssagsl svgsvlesrs sqrsytsvep ngirprkpcn ctksqclkly cdcfangefc
      661 hmcncnncsn nlgneeerqr aiksclernp nafrpkigkg retgddirrh nkgcnckrsg
      721 clknycecye akipcsanck cigcrnveep nlekkslkdl aeaaevrtaq ltlnkaklqi
      781 semafrppat sntgarqpfn fltdkvveit cqclmaqade aernmfddet sqrliieefg
      841 rclkeiiesa hkaeat
//
LOCUS       XP_034190866             823 aa            linear   INV 03-SEP-2025
DEFINITION  protein lin-54 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190866
VERSION     XP_034190866.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334975.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="protein lin-54 homolog isoform X2"
                     /calculated_mol_wt=88564
     Region          <100..453
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          603..>627
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          678..715
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..823
                     /gene="LOC117609088"
                     /coded_by="XM_034334975.2:405..2876"
                     /db_xref="GeneID:117609088"
ORIGIN      
        1 msvnkgqnar alveplalds rtfgdsdlsa lslshnneqy ssndfeafan iqaelecina
       61 eevmstdeeh iiigrniete tivpdvemte iseqveeehi iyttanqnnq nivfqtkptl
      121 qrlptstvqv ksnagqvtqs qsimivspag gqgasqilki shpstasagq lqslaqtlit
      181 aksadgnivq lrsaqpnkpl matshsgsit lgnvhnskta qsaikrtaqs tsqnrnvytk
      241 milagnqaqs gqvlitnsqn enqqaikfln nntssqevtn ptktitlaqa qqmgllstsk
      301 vqhilpstpq kqgiivnklv qssssqpskm tivssntiks ptkilpapvi ntqvktssls
      361 nqqstfscnk ssvqqspqkv iirqsslkpg tvlgsgqvir ipasqnivtg snqvhqiqmp
      421 grqvqyvrlv stpssgttnv vtvgktksqt tlqtvgvgqk iggqqivkvv plntsnqslr
      481 tvapkatlsg sgqrllipat atvgnqskna vaipasalsq lasgqavist nsnvgnivvl
      541 paqyiqqstd evkikpqpia psllgtsqnl qssagslsvg svlesrssqr sytsvepngi
      601 rprkpcnctk sqclklycdc fangefchmc ncnncsnnlg neeerqraik sclernpnaf
      661 rpkigkgret gddirrhnkg cnckrsgclk nycecyeaki pcsanckcig crnveepnle
      721 kkslkdlaea aevrtaqltl nkaklqisem afrppatsnt garqpfnflt dkvveitcqc
      781 lmaqadeaer nmfddetsqr liieefgrcl keiiesahka eat
//
LOCUS       XP_034190867             823 aa            linear   INV 03-SEP-2025
DEFINITION  protein lin-54 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190867
VERSION     XP_034190867.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334976.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="protein lin-54 homolog isoform X2"
                     /calculated_mol_wt=88564
     Region          <100..453
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          603..>627
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          678..715
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..823
                     /gene="LOC117609088"
                     /coded_by="XM_034334976.2:215..2686"
                     /db_xref="GeneID:117609088"
ORIGIN      
        1 msvnkgqnar alveplalds rtfgdsdlsa lslshnneqy ssndfeafan iqaelecina
       61 eevmstdeeh iiigrniete tivpdvemte iseqveeehi iyttanqnnq nivfqtkptl
      121 qrlptstvqv ksnagqvtqs qsimivspag gqgasqilki shpstasagq lqslaqtlit
      181 aksadgnivq lrsaqpnkpl matshsgsit lgnvhnskta qsaikrtaqs tsqnrnvytk
      241 milagnqaqs gqvlitnsqn enqqaikfln nntssqevtn ptktitlaqa qqmgllstsk
      301 vqhilpstpq kqgiivnklv qssssqpskm tivssntiks ptkilpapvi ntqvktssls
      361 nqqstfscnk ssvqqspqkv iirqsslkpg tvlgsgqvir ipasqnivtg snqvhqiqmp
      421 grqvqyvrlv stpssgttnv vtvgktksqt tlqtvgvgqk iggqqivkvv plntsnqslr
      481 tvapkatlsg sgqrllipat atvgnqskna vaipasalsq lasgqavist nsnvgnivvl
      541 paqyiqqstd evkikpqpia psllgtsqnl qssagslsvg svlesrssqr sytsvepngi
      601 rprkpcnctk sqclklycdc fangefchmc ncnncsnnlg neeerqraik sclernpnaf
      661 rpkigkgret gddirrhnkg cnckrsgclk nycecyeaki pcsanckcig crnveepnle
      721 kkslkdlaea aevrtaqltl nkaklqisem afrppatsnt garqpfnflt dkvveitcqc
      781 lmaqadeaer nmfddetsqr liieefgrcl keiiesahka eat
//
LOCUS       XP_034190869             823 aa            linear   INV 03-SEP-2025
DEFINITION  protein lin-54 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190869
VERSION     XP_034190869.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334978.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="protein lin-54 homolog isoform X2"
                     /calculated_mol_wt=88564
     Region          <100..453
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          603..>627
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          678..715
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..823
                     /gene="LOC117609088"
                     /coded_by="XM_034334978.2:316..2787"
                     /db_xref="GeneID:117609088"
ORIGIN      
        1 msvnkgqnar alveplalds rtfgdsdlsa lslshnneqy ssndfeafan iqaelecina
       61 eevmstdeeh iiigrniete tivpdvemte iseqveeehi iyttanqnnq nivfqtkptl
      121 qrlptstvqv ksnagqvtqs qsimivspag gqgasqilki shpstasagq lqslaqtlit
      181 aksadgnivq lrsaqpnkpl matshsgsit lgnvhnskta qsaikrtaqs tsqnrnvytk
      241 milagnqaqs gqvlitnsqn enqqaikfln nntssqevtn ptktitlaqa qqmgllstsk
      301 vqhilpstpq kqgiivnklv qssssqpskm tivssntiks ptkilpapvi ntqvktssls
      361 nqqstfscnk ssvqqspqkv iirqsslkpg tvlgsgqvir ipasqnivtg snqvhqiqmp
      421 grqvqyvrlv stpssgttnv vtvgktksqt tlqtvgvgqk iggqqivkvv plntsnqslr
      481 tvapkatlsg sgqrllipat atvgnqskna vaipasalsq lasgqavist nsnvgnivvl
      541 paqyiqqstd evkikpqpia psllgtsqnl qssagslsvg svlesrssqr sytsvepngi
      601 rprkpcnctk sqclklycdc fangefchmc ncnncsnnlg neeerqraik sclernpnaf
      661 rpkigkgret gddirrhnkg cnckrsgclk nycecyeaki pcsanckcig crnveepnle
      721 kkslkdlaea aevrtaqltl nkaklqisem afrppatsnt garqpfnflt dkvveitcqc
      781 lmaqadeaer nmfddetsqr liieefgrcl keiiesahka eat
//
LOCUS       XP_034190870             823 aa            linear   INV 03-SEP-2025
DEFINITION  protein lin-54 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190870
VERSION     XP_034190870.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="protein lin-54 homolog isoform X2"
                     /calculated_mol_wt=88564
     Region          <100..453
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          603..>627
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          678..715
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..823
                     /gene="LOC117609088"
                     /coded_by="XM_034334979.2:165..2636"
                     /db_xref="GeneID:117609088"
ORIGIN      
        1 msvnkgqnar alveplalds rtfgdsdlsa lslshnneqy ssndfeafan iqaelecina
       61 eevmstdeeh iiigrniete tivpdvemte iseqveeehi iyttanqnnq nivfqtkptl
      121 qrlptstvqv ksnagqvtqs qsimivspag gqgasqilki shpstasagq lqslaqtlit
      181 aksadgnivq lrsaqpnkpl matshsgsit lgnvhnskta qsaikrtaqs tsqnrnvytk
      241 milagnqaqs gqvlitnsqn enqqaikfln nntssqevtn ptktitlaqa qqmgllstsk
      301 vqhilpstpq kqgiivnklv qssssqpskm tivssntiks ptkilpapvi ntqvktssls
      361 nqqstfscnk ssvqqspqkv iirqsslkpg tvlgsgqvir ipasqnivtg snqvhqiqmp
      421 grqvqyvrlv stpssgttnv vtvgktksqt tlqtvgvgqk iggqqivkvv plntsnqslr
      481 tvapkatlsg sgqrllipat atvgnqskna vaipasalsq lasgqavist nsnvgnivvl
      541 paqyiqqstd evkikpqpia psllgtsqnl qssagslsvg svlesrssqr sytsvepngi
      601 rprkpcnctk sqclklycdc fangefchmc ncnncsnnlg neeerqraik sclernpnaf
      661 rpkigkgret gddirrhnkg cnckrsgclk nycecyeaki pcsanckcig crnveepnle
      721 kkslkdlaea aevrtaqltl nkaklqisem afrppatsnt garqpfnflt dkvveitcqc
      781 lmaqadeaer nmfddetsqr liieefgrcl keiiesahka eat
//
LOCUS       XP_034190871             823 aa            linear   INV 03-SEP-2025
DEFINITION  protein lin-54 homolog isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190871
VERSION     XP_034190871.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..823
                     /product="protein lin-54 homolog isoform X2"
                     /calculated_mol_wt=88564
     Region          <100..453
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          603..>627
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          678..715
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..823
                     /gene="LOC117609088"
                     /coded_by="XM_034334980.2:201..2672"
                     /db_xref="GeneID:117609088"
ORIGIN      
        1 msvnkgqnar alveplalds rtfgdsdlsa lslshnneqy ssndfeafan iqaelecina
       61 eevmstdeeh iiigrniete tivpdvemte iseqveeehi iyttanqnnq nivfqtkptl
      121 qrlptstvqv ksnagqvtqs qsimivspag gqgasqilki shpstasagq lqslaqtlit
      181 aksadgnivq lrsaqpnkpl matshsgsit lgnvhnskta qsaikrtaqs tsqnrnvytk
      241 milagnqaqs gqvlitnsqn enqqaikfln nntssqevtn ptktitlaqa qqmgllstsk
      301 vqhilpstpq kqgiivnklv qssssqpskm tivssntiks ptkilpapvi ntqvktssls
      361 nqqstfscnk ssvqqspqkv iirqsslkpg tvlgsgqvir ipasqnivtg snqvhqiqmp
      421 grqvqyvrlv stpssgttnv vtvgktksqt tlqtvgvgqk iggqqivkvv plntsnqslr
      481 tvapkatlsg sgqrllipat atvgnqskna vaipasalsq lasgqavist nsnvgnivvl
      541 paqyiqqstd evkikpqpia psllgtsqnl qssagslsvg svlesrssqr sytsvepngi
      601 rprkpcnctk sqclklycdc fangefchmc ncnncsnnlg neeerqraik sclernpnaf
      661 rpkigkgret gddirrhnkg cnckrsgclk nycecyeaki pcsanckcig crnveepnle
      721 kkslkdlaea aevrtaqltl nkaklqisem afrppatsnt garqpfnflt dkvveitcqc
      781 lmaqadeaer nmfddetsqr liieefgrcl keiiesahka eat
//
LOCUS       XP_034190873             763 aa            linear   INV 03-SEP-2025
DEFINITION  protein lin-54 homolog isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190873
VERSION     XP_034190873.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334982.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..763
                     /product="protein lin-54 homolog isoform X3"
                     /calculated_mol_wt=82094
     Region          <40..393
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          543..>567
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     Region          618..655
                     /region_name="TCR"
                     /note="Tesmin/TSO1-like CXC domain, cysteine-rich domain;
                     pfam03638"
                     /db_xref="CDD:461001"
     CDS             1..763
                     /gene="LOC117609088"
                     /coded_by="XM_034334982.2:54..2345"
                     /db_xref="GeneID:117609088"
ORIGIN      
        1 malmflneeh iiigrniete tivpdvemte iseqveeehi iyttanqnnq nivfqtkptl
       61 qrlptstvqv ksnagqvtqs qsimivspag gqgasqilki shpstasagq lqslaqtlit
      121 aksadgnivq lrsaqpnkpl matshsgsit lgnvhnskta qsaikrtaqs tsqnrnvytk
      181 milagnqaqs gqvlitnsqn enqqaikfln nntssqevtn ptktitlaqa qqmgllstsk
      241 vqhilpstpq kqgiivnklv qssssqpskm tivssntiks ptkilpapvi ntqvktssls
      301 nqqstfscnk ssvqqspqkv iirqsslkpg tvlgsgqvir ipasqnivtg snqvhqiqmp
      361 grqvqyvrlv stpssgttnv vtvgktksqt tlqtvgvgqk iggqqivkvv plntsnqslr
      421 tvapkatlsg sgqrllipat atvgnqskna vaipasalsq lasgqavist nsnvgnivvl
      481 paqyiqqstd evkikpqpia psllgtsqnl qssagslsvg svlesrssqr sytsvepngi
      541 rprkpcnctk sqclklycdc fangefchmc ncnncsnnlg neeerqraik sclernpnaf
      601 rpkigkgret gddirrhnkg cnckrsgclk nycecyeaki pcsanckcig crnveepnle
      661 kkslkdlaea aevrtaqltl nkaklqisem afrppatsnt garqpfnflt dkvveitcqc
      721 lmaqadeaer nmfddetsqr liieefgrcl keiiesahka eat
//
LOCUS       XP_034190859             673 aa            linear   INV 03-SEP-2025
DEFINITION  choline transporter-like protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190859
VERSION     XP_034190859.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334968.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..673
                     /product="choline transporter-like protein 1"
                     /calculated_mol_wt=75714
     Region          288..638
                     /region_name="Choline_transpo"
                     /note="Plasma-membrane choline transporter; pfam04515"
                     /db_xref="CDD:461337"
     CDS             1..673
                     /gene="Ctl1"
                     /coded_by="XM_034334968.2:110..2131"
                     /db_xref="GeneID:117609083"
ORIGIN      
        1 msccsgdddd eqrpdptrvr rctdifwlcc fiafwflmil iaafslvygn pvrlingyds
       61 fgntcgmkin pkfgsmelsg qdtsdkpylf fldinnvtqs lkicvkkcpd rtittmndic
      121 kfyeetgsql chdkpgndfs acsngnrknk tgscpelpvy ksipvlnrci pkaikdvget
      181 iisnlyglin swdvieqilg dlyktwreil slsflafvls lfmiaifhll asivtwivmi
      241 lvsvstiags tllwwtyiei krtldktdpn qlleesvrne raflafsiia tvitvillfl
      301 ltilrkriaf maalfkesak claelpglff qplltfaali lffafwvtvi lclatanypg
      361 tksvqmyknh ifdplnlssv geksafveek kfdfslnsfk tftlveyvds twvkymwwvy
      421 iigliwtsef ivscqdmvia gavahwyfrg kdastspvcs aignlvmyhl gsvacgsfli
      481 tlfklprlil tylyakfeks ketspcaqcg lkcciccfyc lekfirymnh naytvvaieg
      541 thfcnaaria fttlvsnalq iavingvgdf ilflgkcfvt aatgsigllf mkqdprlyfy
      601 aapifvtcvf affiahsiis lyetvidtlf lcicedkqlh gengkwrqsa laqfgssnnv
      661 ngqqaheltp mnt
//
LOCUS       XP_034190880             552 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609092 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190880
VERSION     XP_034190880.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334989.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190880.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..552
                     /product="uncharacterized protein LOC117609092 isoform X1"
                     /calculated_mol_wt=63551
     Region          <414..508
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..552
                     /gene="LOC117609092"
                     /coded_by="XM_034334989.2:377..2035"
                     /db_xref="GeneID:117609092"
ORIGIN      
        1 mllykqilsd atllggkspl scrrwlpper ppppprqigg ipplypwygv gsesqreear
       61 vyyklyrmtn twwlqnfsnm trfarakgsk asnerlpndp tpwhvmkqql venlstsqgy
      121 sektksakel lcekqstsdi tndksnwaef ensyerknns evkkamkrkt qssivkenqn
      181 vssdkhdesn vkvkkrnide vdrsvtevkd kdkksnnqyn nksnkqnkvd elnnetnnvh
      241 dsknktkgml skrqkrnmkr rqnagfetrn dnnsesinnm nndkregmgn gqtrrfdkrf
      301 gdqstdfrng pykfgmqkle nnkkkqkppk irddkehirr kpdigytklv ingveveivk
      361 fdgfpvkked adrlhelkqk mimkgipere invamklerr kaekalarik kcvcfhcrks
      421 ghnlsdcpel gseqagtgic fkcgstehth feckvakpte fryatcficr eqghiakqcp
      481 dnpkgvypqg gcckicgdvt hlkkdcpdli kereentvtv ttiangdiec leenkkilne
      541 nndnkskkiv kf
//
LOCUS       XP_034190881             473 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609092 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190881
VERSION     XP_034190881.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334990.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190881.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="uncharacterized protein LOC117609092 isoform X2"
                     /calculated_mol_wt=54303
     Region          <335..429
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..473
                     /gene="LOC117609092"
                     /coded_by="XM_034334990.2:206..1627"
                     /db_xref="GeneID:117609092"
ORIGIN      
        1 mtrfarakgs kasnerlpnd ptpwhvmkqq lvenlstsqg ysektksake llcekqstsd
       61 itndksnwae fensyerknn sevkkamkrk tqssivkenq nvssdkhdes nvkvkkrnid
      121 evdrsvtevk dkdkksnnqy nnksnkqnkv delnnetnnv hdsknktkgm lskrqkrnmk
      181 rrqnagfetr ndnnsesinn mnndkregmg ngqtrrfdkr fgdqstdfrn gpykfgmqkl
      241 ennkkkqkpp kirddkehir rkpdigytkl vingveveiv kfdgfpvkke dadrlhelkq
      301 kmimkgiper einvamkler rkaekalari kkcvcfhcrk sghnlsdcpe lgseqagtgi
      361 cfkcgsteht hfeckvakpt efryatcfic reqghiakqc pdnpkgvypq ggcckicgdv
      421 thlkkdcpdl ikereentvt vttiangdie cleenkkiln enndnkskki vkf
//
LOCUS       XP_034190883             473 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609092 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190883
VERSION     XP_034190883.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334992.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190883.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..473
                     /product="uncharacterized protein LOC117609092 isoform X2"
                     /calculated_mol_wt=54303
     Region          <335..429
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     CDS             1..473
                     /gene="LOC117609092"
                     /coded_by="XM_034334992.2:180..1601"
                     /db_xref="GeneID:117609092"
ORIGIN      
        1 mtrfarakgs kasnerlpnd ptpwhvmkqq lvenlstsqg ysektksake llcekqstsd
       61 itndksnwae fensyerknn sevkkamkrk tqssivkenq nvssdkhdes nvkvkkrnid
      121 evdrsvtevk dkdkksnnqy nnksnkqnkv delnnetnnv hdsknktkgm lskrqkrnmk
      181 rrqnagfetr ndnnsesinn mnndkregmg ngqtrrfdkr fgdqstdfrn gpykfgmqkl
      241 ennkkkqkpp kirddkehir rkpdigytkl vingveveiv kfdgfpvkke dadrlhelkq
      301 kmimkgiper einvamkler rkaekalari kkcvcfhcrk sghnlsdcpe lgseqagtgi
      361 cfkcgsteht hfeckvakpt efryatcfic reqghiakqc pdnpkgvypq ggcckicgdv
      421 thlkkdcpdl ikereentvt vttiangdie cleenkkiln enndnkskki vkf
//
LOCUS       XP_034190876             532 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein SmydA-8 [Osmia lignaria lignaria].
ACCESSION   XP_034190876
VERSION     XP_034190876.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334985.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190876.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..532
                     /product="SET domain-containing protein SmydA-8"
                     /calculated_mol_wt=60037
     Region          <216..285
                     /region_name="SET_SMYD"
                     /note="SET domain (including SET domain and post-SET
                     domain) found in SET and MYND domain-containing protein,
                     and similar proteins; cd20071"
                     /db_xref="CDD:380997"
     Site            order(226,280,282,285)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380997"
     CDS             1..532
                     /gene="LOC117609089"
                     /coded_by="XM_034334985.2:156..1754"
                     /db_xref="GeneID:117609089"
ORIGIN      
        1 mtmeekpkts atptlkykva rsekfgrylq aaknlqagev ilrekpiavg pitssndyvc
       61 faclrllpki kkgiqylcsk cnvaplcgpg ceeqskhhtp yecdtfknnk klssdnigei
      121 igillplrlw flnqtdpelw krietmeahm derrntsvwr dreinvinvm kalhlipedd
      181 asipdhlqqm cgildvntfe lrspggldgl llrglyvaas fmahdcrgnt hltvdddfql
      241 tvyaslpike neailfnyts sllgtvgrrd hlrggkyfec ecslcsdpye mgshmssilc
      301 prcrkgymgi qnpltrypye kgtawqcdkc rrciggrlvr atinisrtli ddmddydikg
      361 mealtkkllg slhpnhflml slkqkllaay rkevatpnpq kkimqrmlda cremynvlei
      421 vepgisrlkg imlyemhlpl vllanraysa reissaelas rleeagsllk ksltilllep
      481 vdtpegklak ralqelkaln qniadvktit iadqkcshdk kkpqknkstk nk
//
LOCUS       XP_034190878             514 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein SmydA-8 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190878
VERSION     XP_034190878.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334987.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="SET domain-containing protein SmydA-8 isoform
                     X1"
                     /calculated_mol_wt=58047
     Region          <203..289
                     /region_name="SET_SMYD"
                     /note="SET domain (including SET domain and post-SET
                     domain) found in SET and MYND domain-containing protein,
                     and similar proteins; cd20071"
                     /db_xref="CDD:380997"
     Site            order(203..204,213,223..227,262,264)
                     /site_type="active"
                     /db_xref="CDD:380997"
     Site            order(203..204,213,223,261..262,264)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380997"
     Site            order(229,284,286,289)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380997"
     CDS             1..514
                     /gene="LOC117609090"
                     /coded_by="XM_034334987.2:137..1681"
                     /db_xref="GeneID:117609090"
ORIGIN      
        1 msqqssgaaa kleeksteky kilqndkvgr ymvankqlea geemitempf ivgpkaftyp
       61 lclscytpwp pstndkplcs rcgwpvcste cenqpqhkdy ecqifarane kfnvqaalee
      121 tnengvpqle citplrllle teknperwnk evknmeahnk irsqkehwks dhvnvveylr
      181 kqlkldrfse efiqticgil eintfeirtr kgysaralyp tvalmnhscv sntchsisps
      241 dyrirlrttv qvppggelyg sythsllptm lrrehllegk hfacacprcs dptelgthms
      301 slkcnkcdng ivlsldsldp dslwkcthce fttsgpavqk vlhiiqaevd avetisgadg
      361 adaiqerekv ikkyhsvlhp rhafltmlrh sltqmygrvd eyllddlpdv vlehkidmcr
      421 lllqvldvie pgysrirgmt lyelhapllf taktqwnagv ideaalkskm ieasnilkea
      481 atilcleppd tpegeiglva keslieleqs innl
//
LOCUS       XP_034190877             514 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein SmydA-8 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190877
VERSION     XP_034190877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="SET domain-containing protein SmydA-8 isoform
                     X1"
                     /calculated_mol_wt=58047
     Region          <203..289
                     /region_name="SET_SMYD"
                     /note="SET domain (including SET domain and post-SET
                     domain) found in SET and MYND domain-containing protein,
                     and similar proteins; cd20071"
                     /db_xref="CDD:380997"
     Site            order(203..204,213,223..227,262,264)
                     /site_type="active"
                     /db_xref="CDD:380997"
     Site            order(203..204,213,223,261..262,264)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380997"
     Site            order(229,284,286,289)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380997"
     CDS             1..514
                     /gene="LOC117609090"
                     /coded_by="XM_034334986.2:191..1735"
                     /db_xref="GeneID:117609090"
ORIGIN      
        1 msqqssgaaa kleeksteky kilqndkvgr ymvankqlea geemitempf ivgpkaftyp
       61 lclscytpwp pstndkplcs rcgwpvcste cenqpqhkdy ecqifarane kfnvqaalee
      121 tnengvpqle citplrllle teknperwnk evknmeahnk irsqkehwks dhvnvveylr
      181 kqlkldrfse efiqticgil eintfeirtr kgysaralyp tvalmnhscv sntchsisps
      241 dyrirlrttv qvppggelyg sythsllptm lrrehllegk hfacacprcs dptelgthms
      301 slkcnkcdng ivlsldsldp dslwkcthce fttsgpavqk vlhiiqaevd avetisgadg
      361 adaiqerekv ikkyhsvlhp rhafltmlrh sltqmygrvd eyllddlpdv vlehkidmcr
      421 lllqvldvie pgysrirgmt lyelhapllf taktqwnagv ideaalkskm ieasnilkea
      481 atilcleppd tpegeiglva keslieleqs innl
//
LOCUS       XP_034190879             483 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein SmydA-8 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190879
VERSION     XP_034190879.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="SET domain-containing protein SmydA-8 isoform
                     X2"
                     /calculated_mol_wt=54576
     Region          <172..258
                     /region_name="SET_SMYD"
                     /note="SET domain (including SET domain and post-SET
                     domain) found in SET and MYND domain-containing protein,
                     and similar proteins; cd20071"
                     /db_xref="CDD:380997"
     Site            order(172..173,182,192..196,231,233)
                     /site_type="active"
                     /db_xref="CDD:380997"
     Site            order(172..173,182,192,230..231,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380997"
     Site            order(198,253,255,258)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380997"
     CDS             1..483
                     /gene="LOC117609090"
                     /coded_by="XM_034334988.2:163..1614"
                     /db_xref="GeneID:117609090"
ORIGIN      
        1 mvankqleag eemitempfi vgpkaftypl clscytpwpp stndkplcsr cgwpvcstec
       61 enqpqhkdye cqifaranek fnvqaaleet nengvpqlec itplrlllet eknperwnke
      121 vknmeahnki rsqkehwksd hvnvveylrk qlkldrfsee fiqticgile intfeirtrk
      181 gysaralypt valmnhscvs ntchsispsd yrirlrttvq vppggelygs ythsllptml
      241 rrehllegkh facacprcsd ptelgthmss lkcnkcdngi vlsldsldpd slwkcthcef
      301 ttsgpavqkv lhiiqaevda vetisgadga daiqerekvi kkyhsvlhpr hafltmlrhs
      361 ltqmygrvde yllddlpdvv lehkidmcrl llqvldviep gysrirgmtl yelhapllft
      421 aktqwnagvi deaalkskmi easnilkeaa tilcleppdt pegeiglvak eslieleqsi
      481 nnl
//
LOCUS       XP_034190884             454 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609093 [Osmia lignaria lignaria].
ACCESSION   XP_034190884
VERSION     XP_034190884.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190884.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..454
                     /product="uncharacterized protein LOC117609093"
                     /calculated_mol_wt=52477
     Region          97..416
                     /region_name="COG3335"
                     /note="Transposase [Mobilome: prophages, transposons]"
                     /db_xref="CDD:442564"
     CDS             1..454
                     /gene="LOC117609093"
                     /coded_by="XM_034334993.2:162..1526"
                     /db_xref="GeneID:117609093"
ORIGIN      
        1 meeegpgpss asptkrnpsg kfvessqkmm ivdlykslke ehpemkyrpl iqllskqtgi
       61 gqltisktvs eykstgtvks pnkkrprmni lektseedil airrkvhefw frreipnvrk
      121 ileavnqdpe lpsfsessly rllkriefky ivrarnsali dkehivawrq ryiqqiqkyr
      181 seerpiyfld etwvnagdvr rkfrvdtsvt cpenaeqrsl srvipasank gkqliighvg
      241 ssdgfvpgal lcfeakknsa gyhdemnsdt ffdwfrsilp mlrdggvivm dsasyhstkl
      301 ekyptmswkk ndiitwlvak gekvepqyvk aqlvqlsrkl rryeyvidey akqhghtvvr
      361 lppyhcelsp ielawakike yvrarnttck lsdvhalvle avenvsaesw qnfirhttee
      421 enrfcqldqi tdstldesge wvdqsddsds didf
//
LOCUS       XP_034190887             412 aa            linear   INV 03-SEP-2025
DEFINITION  COP9 signalosome subunit 4 [Osmia lignaria lignaria].
ACCESSION   XP_034190887
VERSION     XP_034190887.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334996.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..412
                     /product="COP9 signalosome subunit 4"
                     /calculated_mol_wt=47319
     Region          <92..393
                     /region_name="RPN5"
                     /note="26S proteasome regulatory complex component
                     [Posttranslational modification, protein turnover,
                     chaperones]; COG5071"
                     /db_xref="CDD:227403"
     Region          268..368
                     /region_name="PCI"
                     /note="PCI domain; pfam01399"
                     /db_xref="CDD:460195"
     Region          371..411
                     /region_name="CSN4_RPN5_eIF3a"
                     /note="CSN4/RPN5/eIF3a helix turn helix domain; pfam18420"
                     /db_xref="CDD:375846"
     CDS             1..412
                     /gene="CSN4"
                     /coded_by="XM_034334996.2:144..1382"
                     /db_xref="GeneID:117609094"
ORIGIN      
        1 mvvtaaafvr qqlmtvacsg gshkdqaeky ravldsilll sneemvdalk ifieaivhey
       61 vslvisrqvl tdvsnrllfl pdeiskavsh ytldkiqprv isfeeqvasi rqhladiyer
      121 nqnwreaanv lvgipletgq kqytvdykle tylkiarlyl edddpvqaea finrasllqa
      181 eskneqlqiy ykvcyarvld yrrkfieaaq rynelsyrsi ihedermtal rnalictvla
      241 sagqqrsrml atlfkdercq qlpaysilek myldriirrs elqefeallq phqkactidg
      301 lgstildrav iehnllsask lynnitfeel galleipptk aekiasqmit egrmngyidq
      361 idsivhfetr etlptwdkqi qslcyqvnqi iekiaqtepe wianamenqm vd
//
LOCUS       XP_034190860             386 aa            linear   INV 03-SEP-2025
DEFINITION  ribosome biogenesis protein BRX1 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034190860
VERSION     XP_034190860.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334969.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="ribosome biogenesis protein BRX1 homolog"
                     /calculated_mol_wt=44729
     Region          84..334
                     /region_name="Brix"
                     /note="The Brix domain is found in a number of eukaryotic
                     proteins; cl00935"
                     /db_xref="CDD:469999"
     CDS             1..386
                     /gene="LOC117609084"
                     /coded_by="XM_034334969.2:7..1167"
                     /db_xref="GeneID:117609084"
ORIGIN      
        1 masidgarfr gtnlrrvqhg satrgincif nlklknryci ltmtakkqlk rkrtestnke
       61 nvpetkilpa krmsdepppk kvkwinkqrv lvfatrgisf rhrhlmddik tlmphhrtes
      121 kmertknlqv inemcemknc nktvlfegrr kkdlylwfsn iptgpcakfl veniytmgel
      181 kmtgnclkgs rpllsfdenf ntkphysllk ellvqifgvp nhhpksqpff dhvytfsvld
      241 nriwfrnfqi ltedgglaei gprfvlnpik ifansfggev lwdnpnyisp akfrqsikkk
      301 aagkyinkve qktakelnkp kesyslnpmd eifqsdplek alelkekeve askntsetkk
      361 vkksfkekkh lnkkigvtns kkrikk
//
LOCUS       XP_034190888             328 aa            linear   INV 03-SEP-2025
DEFINITION  erlin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034190888
VERSION     XP_034190888.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334997.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="erlin-1"
                     /calculated_mol_wt=37396
     Region          16..307
                     /region_name="SPFH_like_u3"
                     /note="Uncharacterized family; SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cd03406"
                     /db_xref="CDD:259804"
     CDS             1..328
                     /gene="LOC117609095"
                     /coded_by="XM_034334997.2:171..1157"
                     /db_xref="GeneID:117609095"
ORIGIN      
        1 mfdqsiigic fficlaivfn fslhrieegh vgvyfrggal lpqvsnpgfh mmipllttyr
       61 svqvtlqtde vknvpcgtsg gvmiyfdrie vvnvldansv ynmvrnftad ydqtlifnki
      121 hhelnqfcsv htlhevyidl fdqidenlkt alqkdlndla pglsihavrv tkpkipetir
      181 knyelmegek tkllistqhq kvvekdaetd rkkavieaek eaqvakiqfi qkimekeslq
      241 qiasiedemh larqkshsda nyyqmkmqae snkllltkef lelkkyeala rnakiyygqd
      301 ipkmfmyggc sndpsigstv dvqesimq
//
LOCUS       XP_034190889             328 aa            linear   INV 03-SEP-2025
DEFINITION  erlin-1 [Osmia lignaria lignaria].
ACCESSION   XP_034190889
VERSION     XP_034190889.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334998.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="erlin-1"
                     /calculated_mol_wt=37396
     Region          16..307
                     /region_name="SPFH_like_u3"
                     /note="Uncharacterized family; SPFH (stomatin, prohibitin,
                     flotillin, and HflK/C) superfamily; cd03406"
                     /db_xref="CDD:259804"
     CDS             1..328
                     /gene="LOC117609095"
                     /coded_by="XM_034334998.2:145..1131"
                     /db_xref="GeneID:117609095"
ORIGIN      
        1 mfdqsiigic fficlaivfn fslhrieegh vgvyfrggal lpqvsnpgfh mmipllttyr
       61 svqvtlqtde vknvpcgtsg gvmiyfdrie vvnvldansv ynmvrnftad ydqtlifnki
      121 hhelnqfcsv htlhevyidl fdqidenlkt alqkdlndla pglsihavrv tkpkipetir
      181 knyelmegek tkllistqhq kvvekdaetd rkkavieaek eaqvakiqfi qkimekeslq
      241 qiasiedemh larqkshsda nyyqmkmqae snkllltkef lelkkyeala rnakiyygqd
      301 ipkmfmyggc sndpsigstv dvqesimq
//
LOCUS       XP_034190861             322 aa            linear   INV 03-SEP-2025
DEFINITION  GDP-L-fucose synthase-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034190861
VERSION     XP_034190861.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334970.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190861.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="GDP-L-fucose synthase-like protein"
                     /calculated_mol_wt=36575
     Region          8..314
                     /region_name="GDP_FS_SDR_e"
                     /note="GDP-fucose synthetase, extended (e) SDRs; cd05239"
                     /db_xref="CDD:187550"
     Site            order(12,14..17,40,43..46,66..68,70,90,109..111,140,144,
                     167..168,170)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:187550"
     Site            order(87,111,140,144)
                     /site_type="active"
                     /db_xref="CDD:187550"
     Site            order(111,140,169,188,208,216)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187550"
     CDS             1..322
                     /gene="Gmer"
                     /coded_by="XM_034334970.2:160..1128"
                     /db_xref="GeneID:117609085"
ORIGIN      
        1 msenkkiilv tggtglvgka iqniieidkn enerwifvgs kdanlcdkes tkklfekhkp
       61 thvihlaamv gglfhnmshn ldflrnnlqm ndnvlqtahe ngvvkviscl stcifpdktt
      121 ypidesmihn gpphpsnygy syakrlidia nrgyyeqygr lytsiipcnv fgpndnfhlq
      181 ashvipglmr rlydlikdgn tedktftilg sgkplrqfiy sldlakliiw vlreynsvep
      241 iilsvdesqe vtitqvaeai skafnfkgkl tydrsaadgq ykktasnakl rkylpdfqft
      301 pfnqaiqetv dwyiknydra rn
//
LOCUS       XP_034190862             322 aa            linear   INV 03-SEP-2025
DEFINITION  GDP-L-fucose synthase-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034190862
VERSION     XP_034190862.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334971.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190862.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="GDP-L-fucose synthase-like protein"
                     /calculated_mol_wt=36575
     Region          8..314
                     /region_name="GDP_FS_SDR_e"
                     /note="GDP-fucose synthetase, extended (e) SDRs; cd05239"
                     /db_xref="CDD:187550"
     Site            order(12,14..17,40,43..46,66..68,70,90,109..111,140,144,
                     167..168,170)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:187550"
     Site            order(87,111,140,144)
                     /site_type="active"
                     /db_xref="CDD:187550"
     Site            order(111,140,169,188,208,216)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187550"
     CDS             1..322
                     /gene="Gmer"
                     /coded_by="XM_034334971.2:161..1129"
                     /db_xref="GeneID:117609085"
ORIGIN      
        1 msenkkiilv tggtglvgka iqniieidkn enerwifvgs kdanlcdkes tkklfekhkp
       61 thvihlaamv gglfhnmshn ldflrnnlqm ndnvlqtahe ngvvkviscl stcifpdktt
      121 ypidesmihn gpphpsnygy syakrlidia nrgyyeqygr lytsiipcnv fgpndnfhlq
      181 ashvipglmr rlydlikdgn tedktftilg sgkplrqfiy sldlakliiw vlreynsvep
      241 iilsvdesqe vtitqvaeai skafnfkgkl tydrsaadgq ykktasnakl rkylpdfqft
      301 pfnqaiqetv dwyiknydra rn
//
LOCUS       XP_034190863             322 aa            linear   INV 03-SEP-2025
DEFINITION  GDP-L-fucose synthase-like protein [Osmia lignaria lignaria].
ACCESSION   XP_034190863
VERSION     XP_034190863.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334972.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190863.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="GDP-L-fucose synthase-like protein"
                     /calculated_mol_wt=36575
     Region          8..314
                     /region_name="GDP_FS_SDR_e"
                     /note="GDP-fucose synthetase, extended (e) SDRs; cd05239"
                     /db_xref="CDD:187550"
     Site            order(12,14..17,40,43..46,66..68,70,90,109..111,140,144,
                     167..168,170)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:187550"
     Site            order(87,111,140,144)
                     /site_type="active"
                     /db_xref="CDD:187550"
     Site            order(111,140,169,188,208,216)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187550"
     CDS             1..322
                     /gene="Gmer"
                     /coded_by="XM_034334972.2:153..1121"
                     /db_xref="GeneID:117609085"
ORIGIN      
        1 msenkkiilv tggtglvgka iqniieidkn enerwifvgs kdanlcdkes tkklfekhkp
       61 thvihlaamv gglfhnmshn ldflrnnlqm ndnvlqtahe ngvvkviscl stcifpdktt
      121 ypidesmihn gpphpsnygy syakrlidia nrgyyeqygr lytsiipcnv fgpndnfhlq
      181 ashvipglmr rlydlikdgn tedktftilg sgkplrqfiy sldlakliiw vlreynsvep
      241 iilsvdesqe vtitqvaeai skafnfkgkl tydrsaadgq ykktasnakl rkylpdfqft
      301 pfnqaiqetv dwyiknydra rn
//
LOCUS       XP_034190890             283 aa            linear   INV 03-SEP-2025
DEFINITION  voltage-dependent anion-selective channel [Osmia lignaria
            lignaria].
ACCESSION   XP_034190890
VERSION     XP_034190890.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334999.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="voltage-dependent anion-selective channel"
                     /calculated_mol_wt=30450
     Region          3..282
                     /region_name="Porin3_VDAC"
                     /note="Voltage-dependent anion channel of the outer
                     mitochondrial membrane; cd07306"
                     /db_xref="CDD:132767"
     Site            order(15,19,45,60,83,154,282)
                     /site_type="active"
                     /note="putative determinants of voltage gating [active]"
                     /db_xref="CDD:132767"
     Site            order(26,28,257,259,277)
                     /site_type="other"
                     /note="putative dimerization interface [polypeptide
                     binding]"
                     /db_xref="CDD:132767"
     CDS             1..283
                     /gene="LOC117609096"
                     /coded_by="XM_034334999.2:160..1011"
                     /db_xref="GeneID:117609096"
ORIGIN      
        1 mapptytelg knardlfsdg yhfglikldi ksktksgvef atggvsnqdt gkvfgtletk
       61 ykiddyglkf sekwntdntl atdvtfsdkl lkgltlgygc tfspqtgtkt gklktsykhd
      121 nvsatadfdl slsagplvna ssvigyqgwl agyqacfdtq rnkltknnfa lgfiasdftl
      181 haavnngcdf sgliyhkiks dlegainlew nssnnvtqfg iatrynldad asvrakinsn
      241 lqvglgyqqk lregitltls tnidgknfgs gghkvglald lqa
//
LOCUS       XP_034190891             283 aa            linear   INV 03-SEP-2025
DEFINITION  voltage-dependent anion-selective channel [Osmia lignaria
            lignaria].
ACCESSION   XP_034190891
VERSION     XP_034190891.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335000.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="voltage-dependent anion-selective channel"
                     /calculated_mol_wt=30450
     Region          3..282
                     /region_name="Porin3_VDAC"
                     /note="Voltage-dependent anion channel of the outer
                     mitochondrial membrane; cd07306"
                     /db_xref="CDD:132767"
     Site            order(15,19,45,60,83,154,282)
                     /site_type="active"
                     /note="putative determinants of voltage gating [active]"
                     /db_xref="CDD:132767"
     Site            order(26,28,257,259,277)
                     /site_type="other"
                     /note="putative dimerization interface [polypeptide
                     binding]"
                     /db_xref="CDD:132767"
     CDS             1..283
                     /gene="LOC117609096"
                     /coded_by="XM_034335000.2:163..1014"
                     /db_xref="GeneID:117609096"
ORIGIN      
        1 mapptytelg knardlfsdg yhfglikldi ksktksgvef atggvsnqdt gkvfgtletk
       61 ykiddyglkf sekwntdntl atdvtfsdkl lkgltlgygc tfspqtgtkt gklktsykhd
      121 nvsatadfdl slsagplvna ssvigyqgwl agyqacfdtq rnkltknnfa lgfiasdftl
      181 haavnngcdf sgliyhkiks dlegainlew nssnnvtqfg iatrynldad asvrakinsn
      241 lqvglgyqqk lregitltls tnidgknfgs gghkvglald lqa
//
LOCUS       XP_034190758             195 aa            linear   INV 03-SEP-2025
DEFINITION  succinate dehydrogenase, subunit D [Osmia lignaria lignaria].
ACCESSION   XP_034190758
VERSION     XP_034190758.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334867.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..195
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..195
                     /product="succinate dehydrogenase, subunit D"
                     /calculated_mol_wt=21039
     Region          80..182
                     /region_name="SQR_QFR_TM"
                     /note="Succinate:quinone oxidoreductase (SQR) and
                     Quinol:fumarate reductase (QFR) family, transmembrane
                     subunits; SQR catalyzes the oxidation of succinate to
                     fumarate coupled to the reduction of quinone to quinol,
                     while QFR catalyzes the reverse reaction. SQR; cl00881"
                     /db_xref="CDD:469971"
     Site            order(89,129)
                     /site_type="active"
                     /note="Iron-sulfur protein interface [active]"
                     /db_xref="CDD:239573"
     Site            order(96,122,126)
                     /site_type="other"
                     /note="proximal heme binding site [chemical binding]"
                     /db_xref="CDD:239573"
     CDS             1..195
                     /gene="SdhD"
                     /coded_by="XM_034334867.2:121..708"
                     /db_xref="GeneID:117609030"
ORIGIN      
        1 mivkkiiasn ifhkvrqles lsktnllans cklpqfgrts ttlanpkqcl hsnvmnngtk
       61 fpvigtvglq lsrkastins dhvrlwvfek lvssalpvii paalimenaa ldgimsvliv
      121 mhthwgleai itdyarpiiv gtvvpkvlhl slfilsavtl cgllvlindg pgvsraiknf
      181 waigkkpkes vasde
//
LOCUS       XP_034190764             139 aa            linear   INV 03-SEP-2025
DEFINITION  pterin-4a-carbinolamine dehydratase [Osmia lignaria lignaria].
ACCESSION   XP_034190764
VERSION     XP_034190764.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334873.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..139
                     /product="pterin-4a-carbinolamine dehydratase"
                     /calculated_mol_wt=16112
     Region          58..133
                     /region_name="PCD_DCoH_subfamily_b"
                     /note="PCD_DCoH: The bifunctional protein
                     pterin-4alpha-carbinolamine dehydratase (PCD), also known
                     as DCoH (dimerization cofactor of hepatocyte nuclear
                     factor-1), is both a transcription activator and a
                     metabolic enzyme. DCoH stimulates gene expression by...;
                     cd00914"
                     /db_xref="CDD:238456"
     Site            order(77,81,97,100,114)
                     /site_type="other"
                     /note="aromatic arch"
                     /db_xref="CDD:238456"
     Site            order(77,92,97..104)
                     /site_type="other"
                     /note="DCoH dimer interaction site [polypeptide binding]"
                     /db_xref="CDD:238456"
     Site            order(78..79,85..86,89,92..93)
                     /site_type="other"
                     /note="DCoH /HNF-1 dimer interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:238456"
     Site            order(78,85..86,89,93)
                     /site_type="other"
                     /note="DCoH tetramer interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:238456"
     Site            order(95..97,112,114..115)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238456"
     CDS             1..139
                     /gene="Pcd"
                     /coded_by="XM_034334873.2:245..664"
                     /db_xref="GeneID:117609037"
ORIGIN      
        1 masltrmrli nsrgilqeei fdifyrrnss ttvlkkrkmg kltkeerdnd lkplltngws
       61 vqenrdaiyk eflfnnfnqa fgfmtrvalq aekmdhhpew fnvynkvnit lsshdvngls
      121 prdiklatfi dkvakaegk
//
LOCUS       XP_076548054             139 aa            linear   INV 03-SEP-2025
DEFINITION  pterin-4a-carbinolamine dehydratase [Osmia lignaria lignaria].
ACCESSION   XP_076548054
VERSION     XP_076548054.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691939.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..139
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..139
                     /product="pterin-4a-carbinolamine dehydratase"
                     /calculated_mol_wt=16112
     Region          58..133
                     /region_name="PCD_DCoH_subfamily_b"
                     /note="PCD_DCoH: The bifunctional protein
                     pterin-4alpha-carbinolamine dehydratase (PCD), also known
                     as DCoH (dimerization cofactor of hepatocyte nuclear
                     factor-1), is both a transcription activator and a
                     metabolic enzyme. DCoH stimulates gene expression by...;
                     cd00914"
                     /db_xref="CDD:238456"
     Site            order(77,81,97,100,114)
                     /site_type="other"
                     /note="aromatic arch"
                     /db_xref="CDD:238456"
     Site            order(77,92,97..104)
                     /site_type="other"
                     /note="DCoH dimer interaction site [polypeptide binding]"
                     /db_xref="CDD:238456"
     Site            order(78..79,85..86,89,92..93)
                     /site_type="other"
                     /note="DCoH /HNF-1 dimer interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:238456"
     Site            order(78,85..86,89,93)
                     /site_type="other"
                     /note="DCoH tetramer interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:238456"
     Site            order(95..97,112,114..115)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238456"
     CDS             1..139
                     /gene="Pcd"
                     /coded_by="XM_076691939.1:182..601"
                     /db_xref="GeneID:117609037"
ORIGIN      
        1 masltrmrli nsrgilqeei fdifyrrnss ttvlkkrkmg kltkeerdnd lkplltngws
       61 vqenrdaiyk eflfnnfnqa fgfmtrvalq aekmdhhpew fnvynkvnit lsshdvngls
      121 prdiklatfi dkvakaegk
//
LOCUS       XP_034190759             115 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L36 [Osmia lignaria lignaria].
ACCESSION   XP_034190759
VERSION     XP_034190759.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334868.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..115
                     /product="ribosomal protein L36"
                     /calculated_mol_wt=13273
     Region          4..110
                     /region_name="Ribosomal_L36e"
                     /note="Ribosomal protein L36e; pfam01158"
                     /db_xref="CDD:460088"
     CDS             1..115
                     /gene="RpL36"
                     /coded_by="XM_034334868.2:145..492"
                     /db_xref="GeneID:117609032"
ORIGIN      
        1 mapryelavg lsrghkttki rvaknknekk ktvcvlparf kgrqtkhskf irdlirevtg
       61 hapyekrame llkvskdkra lkflkrrlgt hirakrkree lgnilvqmrk aaahh
//
LOCUS       XP_034190864             110 aa            linear   INV 03-SEP-2025
DEFINITION  PAT complex subunit Asterix [Osmia lignaria lignaria].
ACCESSION   XP_034190864
VERSION     XP_034190864.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334973.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..110
                     /product="PAT complex subunit Asterix"
                     /calculated_mol_wt=12328
     Region          3..102
                     /region_name="ASTER"
                     /note="PAT complex subunit Asterix; pfam03669"
                     /db_xref="CDD:461012"
     CDS             1..110
                     /gene="LOC117609086"
                     /coded_by="XM_034334973.2:162..494"
                     /db_xref="GeneID:117609086"
ORIGIN      
        1 mnsssdprrp erevrykpnv mnsqvqlgdn stpdymnilg mvfsmcglmm rlkwcawval
       61 ycscisfans kvcddtkqil ssfmlsisav vmsylqnpqp mtppwasamq
//
LOCUS       XP_034186856            1177 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: pleckstrin homology domain-containing protein
            pruning defect 1 [Osmia lignaria lignaria].
ACCESSION   XP_034186856
VERSION     XP_034186856.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330965.2
KEYWORDS    RefSeq; corrected model.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186856.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1177
                     /product="LOW QUALITY PROTEIN: pleckstrin homology
                     domain-containing protein pruning defect 1"
                     /calculated_mol_wt=133352
     Region          62..199
                     /region_name="RUN_PLEKHM2"
                     /note="RUN domain found in pleckstrin homology
                     domain-containing family M member 2 (PLEKHM2) and similar
                     proteins; cd17680"
                     /db_xref="CDD:439042"
     Region          932..1039
                     /region_name="PH_SKIP"
                     /note="SifA and kinesin-interacting protein Pleckstrin
                     homology (PH) domain; cd13309"
                     /db_xref="CDD:270119"
     Site            order(986,991..995,997..998,1010,1022,1033,1036)
                     /site_type="other"
                     /note="SifA binding site [polypeptide binding]"
                     /db_xref="CDD:270119"
     CDS             1..1177
                     /gene="prd1"
                     /coded_by="XM_034330965.2:16..3549"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 2 bases in 1 codon"
                     /db_xref="GeneID:117607357"
ORIGIN      
        1 mcqcgstnda sevfslgclr ffpsfctkvv lsldknhvrl eiempsqedm dlctdkvlsk
       61 grilqeltka vklvyaqsly gtvvldgdsw lvywldralr hglrverhgy wgtarelshq
      121 dtvvvihalq svltsigkgr awlyhtlseg sfesylacll rdtktlkkqy fphalvrdvd
      181 ktnqlvnlla glenvsftlq ldvtyldtcn ympqrpmsgt ilslnlrsss vssslaspgd
      241 sgvafdsdmd vanetdassy keedfhledt rryrnnrykt minncmednk nfsssdssed
      301 gktptqspgv ttkfqtvmvt ksdianqnlt rklddnelnc ssldtlkdyd fysksldesk
      361 ldkinnkndn vvkelnkrst yysdgsysfk rnidprnsyv inndtnllel smtisdceni
      421 eppygilnti nmtdasilss ssseaivqrr qrkkkrgesk krvsfhedil ktmkldddes
      481 fadfsmsflt pnsvqkkdaq kgryswcahg dspyvqksvn arnahsdyys ysssssvfds
      541 dcdkkgslkt ceqlpcqsnc kcacglslse rgvpegqedp gkalrpkmei eleeneaqea
      601 yivekqygni vedppskvem pcpsnskkyt sssdwsdvds tttseaderk nsrhlaspsk
      661 krnmtsiltg essnkllapp lrqapsktsl lsrflksite rkfevkqakk pqkanplyik
      721 gvkasydsfk dfndnldrei qenvaaekle fsgekislkl relfkkhiyr dkteelykvy
      781 kvrssymtng eskpmiallt dktlyltgsk inhtysnqfv ipyneldvim vgpnaqtill
      841 snadyemqyl fstgcsqtts elishleiam rrsptkpklp avkelnyedm hilrhsilcd
      901 tavhpdekie hynliymede ymsppstpcg ptkegdlmyr phtyqqlhpn aptnpweagy
      961 fvlkggvvym ftdanqrlpk raiplkgglc qgcrripnsh rphtfeillk pnkafqfaap
     1021 deyvasewlq sfvqsasglf daskkkeplp cslitttqhl vamkevfpgk qrgqtlscas
     1081 vkdltafrvp ltqqswcile facrevhess gdwviyftny telctfreil etlwadanlg
     1141 efpmvtlppd dklhrrcsda skelelawry llpsvid
//
LOCUS       XP_034186858             778 aa            linear   INV 03-SEP-2025
DEFINITION  poly(ADP-ribose) glycohydrolase [Osmia lignaria lignaria].
ACCESSION   XP_034186858
VERSION     XP_034186858.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330967.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..778
                     /product="poly(ADP-ribose) glycohydrolase"
                     /calculated_mol_wt=88644
     Region          183..520
                     /region_name="PARG_cat"
                     /note="Poly (ADP-ribose) glycohydrolase (PARG); pfam05028"
                     /db_xref="CDD:461528"
     CDS             1..778
                     /gene="LOC117607358"
                     /coded_by="XM_034330967.2:5..2341"
                     /db_xref="GeneID:117607358"
ORIGIN      
        1 msdpglsfde ealspdifsd vidnltnnpv nsddsnsmsv dvnepepadd vpewkgmsmd
       61 eirkglgvyd cqelppicps sthtvlvslp lpargtpkpy pmhqvdkwsq dyvrmphskh
      121 svypidengt rryrnrweii qeallqnfvs tqqlesails ynhmyaqrwd ftalhhffse
      181 vvdeeeanif fevlfpkmvq lalqlpvlit gpvpllkrht ngtislsqlq iasllanaff
      241 ctfprrnstn pqseygrypy infnrlfsay kqekwnkces vtekmkcifh yfrrvtskap
      301 egmvtiqrry ipksdcpkwd dqvqkllplh itsrgtieae gtgllqvdfa nkyvgggvlg
      361 lgcvqeeirf vicpelmvtm lvteelddte alivcgvery skykgysssf kwigdfvdet
      421 prdssgrrlt sivaidalyf thpqsqfnis nitrelnkay vgfvgcegnk nnlppvgtgn
      481 wgcgafrgnp klkvllqlma tavtgrsmvy ftfgdtalrd eiaemymhfv kheitiglif
      541 sllrqyqefa ptgnldfyrf lynrskikpl sqnlnknvht mfsttkevvl kknksitnge
      601 krpvhldikk yqnteeeniq twlsnideea tnkkktdipv eeylevknia ekepvqnkds
      661 tskkeqiqdd dntekkrkqs lwkilgdtvq ktnqerrlsg lelfdaeqel vscqagnscs
      721 estenknwps vvtftdshss hesikisgen ptsscnrnds ptkkagqrki sdffqrts
//
LOCUS       XP_034186866             296 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein L47 [Osmia lignaria lignaria].
ACCESSION   XP_034186866
VERSION     XP_034186866.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330975.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186866.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..296
                     /product="mitochondrial ribosomal protein L47"
                     /calculated_mol_wt=35186
     Region          87..172
                     /region_name="MRP-L47"
                     /note="Mitochondrial 39-S ribosomal protein L47 (MRP-L47);
                     pfam06984"
                     /db_xref="CDD:369158"
     CDS             1..296
                     /gene="mRpL47"
                     /coded_by="XM_034330975.2:11..901"
                     /db_xref="GeneID:117607362"
ORIGIN      
        1 msqldlnsvf isvvtgrqpv frfwfinkma altkavqvsk tvnnvtklft tlslspivsp
       61 isspvyirrv ptfrcalfhs tskrndlmef fddkknwged kvqvgrswem delrlksned
      121 lhklwfvllk ernmlltmee aykqewkyfp nperidkved smvnletvvr ernkayhmle
      181 tgrsgerptk lrynplglrh lykmrqypip kfvntkwhkt hkfgyggiav qkflrlyrek
      241 lwnqkrkmrn hqdnqvavlm rifpnldida vkekyplani erlkrsrksd ghfvkd
//
LOCUS       XP_076548055            1623 aa            linear   INV 03-SEP-2025
DEFINITION  PWP1 homolog no child left behind isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548055
VERSION     XP_076548055.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691940.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1623
                     /product="PWP1 homolog no child left behind isoform X1"
                     /calculated_mol_wt=178679
     Region          <1082..1177
                     /region_name="CAP-ZIP_m"
                     /note="WASH complex subunit CAP-Z interacting, central
                     region; pfam15255"
                     /db_xref="CDD:464595"
     CDS             1..1623
                     /gene="nclb"
                     /coded_by="XM_076691940.1:259..5130"
                     /db_xref="GeneID:117605737"
ORIGIN      
        1 mdkswdhpwt teemrkkrre wslagdagll khlqqfsenl vskankveet ldslttqlhe
       61 ttifidnvtn rslalantqf iesrvqeddi eiekkvetsv qeckdqdlva aeliatvsds
      121 ikqglnimne kykemefvds dseeeededn kvvlsvvgpn npyqdrplpy vigsekwkns
      181 skiglessss seseqvdeee sdseaddtaa fknlnldpks nlvgllpsln dtynkrndit
      241 ymdndkmdvv sqnnidsise svtpnnsvpk vslsndsapn faeelakrlg tvrqaqkptv
      301 mdeksdasin rfkedlfape edenvsidks knilseskkf vndqssenlw kekpikpykn
      361 niipasidvp ppisaistkp ksaiddlfad adsedsddif sskntvktit knkhlsnsnv
      421 ladkenaqkk fldivapthm atstpdtdvn ttnlfsdded mgdlfgsskn qpqnkkkpvg
      481 gisilgnilt tdvenklssr isrtqssgss gtntpttygs stnnepvndr srnnvsndnv
      541 nerdsieiar nnavnlavpn esnyssgisn pswsinntvg ssiginspqm tstrlkseei
      601 yrervvsdsl fsaqtrnpis vtpnstnnqs qresnenvls teqddvfene dlfgppplpk
      661 adsklakskv sslfddsdsg delfsttssg srsqkssdff tsgvqhpeka kttprkglfd
      721 eeidifgnkd spdidifgia skspakpenn isswkipdis ndglfasnak dtivksntme
      781 rnqsvgvtkk islfddeedi ddgdlfgtkp vkneaksesr ilkndddekk kkekehkeed
      841 elfsakkqan dkssigttas gtdnveqdvs tlssekvdkn vlsgnslfsn tigthglvfe
      901 dddyddlfst kstksvikee tkkesvrsks etiddtketl akeiktfdes nifakaeeps
      961 afdttsnkve ekndkdvhrv ssaepsvaed ienetkkspp ksldiqtvas sspseendqg
     1021 tkrtvsgkik nlmgkmgdlk ilspmdvppl wrrseektde edstadrdsd dggcisiqgh
     1081 sslsasedsa tqkqslqsti sgesnvesai sfdepvqvet lsttasktrv riqakrrpqs
     1141 ryarksaltq igidfdtvda sesnsqhdsh gnpsqnifnk dpvshtvsht vatssdrlis
     1201 dnlhrttdan ifltaddkse lgslskessm sankntllsp stdeedlfdv ppdlpedpqk
     1261 edtlfgrapi lspveqvise rvstsfkplk dthikqingt dggieentqk teennvldep
     1321 snnsngkitv phkseeefke dnieakdeln dvvdplrdst hdplkdpsql fafvtktpsp
     1381 ekgknllfse ddslfssggk ksteeqttkk svldlfsddm ggdlfstplt kplkkplkda
     1441 kislfdddeq ddeddslfgp vvkkssvkse sikkpavqqs gkklslfddd dndtnlfses
     1501 seqtqksdsg svqeqsnkne ifasivepvk tshitdifad qssgeddifa sktvpkkvvt
     1561 skslfpsddd dddgdnifgk ksttetyvkt tetrstikks vtkdlkktae kigddplsil
     1621 qdd
//
LOCUS       XP_076548056            1621 aa            linear   INV 03-SEP-2025
DEFINITION  PWP1 homolog no child left behind isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548056
VERSION     XP_076548056.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691941.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1621
                     /product="PWP1 homolog no child left behind isoform X2"
                     /calculated_mol_wt=178643
     Region          <1087..1175
                     /region_name="CAP-ZIP_m"
                     /note="WASH complex subunit CAP-Z interacting, central
                     region; pfam15255"
                     /db_xref="CDD:464595"
     CDS             1..1621
                     /gene="nclb"
                     /coded_by="XM_076691941.1:150..5015"
                     /db_xref="GeneID:117605737"
ORIGIN      
        1 mdisdemdks wdhpwtteem rkkrrewsla gdagllkhlq qfsenlvska nkveetldsl
       61 ttqlhettif idnvtnrsla lantqfiesr vqeddieiek kvetsvqeck dqdlvaaeli
      121 atvsdsikqg lnimnekyke mefvdsdsee eedednkvvl svvgpnnpyq drplpyvigs
      181 ekwknsskig lesssssese qvdeeesdse addtaafknl nldpksnlvg llpslndtyn
      241 krnditymdn dkmdvvsqnn idsisesvtp nnsvpkvsls ndsapnfaee lakrlgtvrq
      301 aqkptvmdek sdasinrfke dlfapeeden vsidksknil seskkfvndq ssenlwkekp
      361 ikpyknniip asidvpppis aistkpksai ddlfadadse dsddifsskn tvktitknkh
      421 lsnsnvladk enaqkkfldi vapthmatst pdtdvnttnl fsddedmgdl fgssknqpqn
      481 kkkpvggisi lgnilttdve nklssrisrt qssgssgtnt pttygsstnn epvndrsrnn
      541 vsndnvnerd sieiarnnav nlavpnesny ssgisnpsws inntvgssig inspqmtstr
      601 lkseeiyrer vvsdslfsaq trnpisvtpn stnnqsqres nenvlsteqd dvfenedlfg
      661 ppplpkadsk lakskvsslf ddsdsgdelf sttssgsrsq kssdfftsgv qhpekakttp
      721 rkglfdeeid ifgnkdspdi difgiasksp akpennissw kipdisndgl fasnakdtiv
      781 ksntmernqs vgvtkkislf ddeediddgd lfgtkpvkne aksesrilkn dddekkkkek
      841 ehkeedelfs akkqandkss igttasgtdn veqdvstlss ekvdknvlsg nslfsntigt
      901 hglvfedddy ddlfstkstk svikeetkke svrsksetid dtketlakei ktfdesnifa
      961 kaeepsafdt tsnkveeknd kdvhrvssae psvaediene tkksppksld iqtvasssps
     1021 eendqgtkrt vsgkiknlmg kmgdlkilsp mdvpplwrrs eektdeedst adrdsddggc
     1081 isiqedsatq kqslqstisg esnvesaisf depvqvetls ttasktrvri qakrrpqsry
     1141 arksaltqig idfdtvdase snsqhdshgn psqnifnkdp vshtvshtva tssdrlisdn
     1201 lhrttdanif ltaddkselg slskessmsa nkntllspst deedlfdvpp dlpedpqked
     1261 tlfgrapils pveqviserv stsfkplkdt hikqingtdg gieentqkte ennvldepsn
     1321 nsngkitvph kseeefkedn ieakdelndv vdplrdsthd plkdpsqlfa fvtktpspek
     1381 gknllfsedd slfssggkks teeqttkksv ldlfsddmgg dlfstpltkp lkkplkdaki
     1441 slfdddeqdd eddslfgpvv kkssvksesi kkpavqqsgk klslfddddn dtnlfsesse
     1501 qtqksdsgsv qeqsnkneif asivepvkts hitdifadqs sgeddifask tvpkkvvtsk
     1561 slfpsddddd dgdnifgkks ttetyvktte trstikksvt kdlkktaeki gddplsilqd
     1621 d
//
LOCUS       XP_076548057            1496 aa            linear   INV 03-SEP-2025
DEFINITION  PWP1 homolog no child left behind isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548057
VERSION     XP_076548057.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691942.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1496
                     /product="PWP1 homolog no child left behind isoform X3"
                     /calculated_mol_wt=164181
     Region          <955..1050
                     /region_name="CAP-ZIP_m"
                     /note="WASH complex subunit CAP-Z interacting, central
                     region; pfam15255"
                     /db_xref="CDD:464595"
     CDS             1..1496
                     /gene="nclb"
                     /coded_by="XM_076691942.1:394..4884"
                     /db_xref="GeneID:117605737"
ORIGIN      
        1 mnekykemef vdsdseeeed ednkvvlsvv gpnnpyqdrp lpyvigsekw knsskigles
       61 sssseseqvd eeesdseadd taafknlnld pksnlvgllp slndtynkrn ditymdndkm
      121 dvvsqnnids isesvtpnns vpkvslsnds apnfaeelak rlgtvrqaqk ptvmdeksda
      181 sinrfkedlf apeedenvsi dksknilses kkfvndqsse nlwkekpikp yknniipasi
      241 dvpppisais tkpksaiddl fadadsedsd difsskntvk titknkhlsn snvladkena
      301 qkkfldivap thmatstpdt dvnttnlfsd dedmgdlfgs sknqpqnkkk pvggisilgn
      361 ilttdvenkl ssrisrtqss gssgtntptt ygsstnnepv ndrsrnnvsn dnvnerdsie
      421 iarnnavnla vpnesnyssg isnpswsinn tvgssigins pqmtstrlks eeiyrervvs
      481 dslfsaqtrn pisvtpnstn nqsqresnen vlsteqddvf enedlfgppp lpkadsklak
      541 skvsslfdds dsgdelfstt ssgsrsqkss dfftsgvqhp ekakttprkg lfdeeidifg
      601 nkdspdidif giaskspakp ennisswkip disndglfas nakdtivksn tmernqsvgv
      661 tkkislfdde ediddgdlfg tkpvkneaks esrilknddd ekkkkekehk eedelfsakk
      721 qandkssigt tasgtdnveq dvstlssekv dknvlsgnsl fsntigthgl vfedddyddl
      781 fstkstksvi keetkkesvr sksetiddtk etlakeiktf desnifakae epsafdttsn
      841 kveekndkdv hrvssaepsv aedienetkk sppksldiqt vassspseen dqgtkrtvsg
      901 kiknlmgkmg dlkilspmdv pplwrrseek tdeedstadr dsddggcisi qghsslsase
      961 dsatqkqslq stisgesnve saisfdepvq vetlsttask trvriqakrr pqsryarksa
     1021 ltqigidfdt vdasesnsqh dshgnpsqni fnkdpvshtv shtvatssdr lisdnlhrtt
     1081 danifltadd kselgslske ssmsankntl lspstdeedl fdvppdlped pqkedtlfgr
     1141 apilspveqv iservstsfk plkdthikqi ngtdggieen tqkteennvl depsnnsngk
     1201 itvphkseee fkednieakd elndvvdplr dsthdplkdp sqlfafvtkt pspekgknll
     1261 fseddslfss ggkksteeqt tkksvldlfs ddmggdlfst pltkplkkpl kdakislfdd
     1321 deqddeddsl fgpvvkkssv ksesikkpav qqsgkklslf ddddndtnlf sesseqtqks
     1381 dsgsvqeqsn kneifasive pvktshitdi fadqssgedd ifasktvpkk vvtskslfps
     1441 ddddddgdni fgkksttety vkttetrsti kksvtkdlkk taekigddpl silqdd
//
LOCUS       XP_076548058            1496 aa            linear   INV 03-SEP-2025
DEFINITION  PWP1 homolog no child left behind isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548058
VERSION     XP_076548058.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691943.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1496
                     /product="PWP1 homolog no child left behind isoform X3"
                     /calculated_mol_wt=164181
     Region          <955..1050
                     /region_name="CAP-ZIP_m"
                     /note="WASH complex subunit CAP-Z interacting, central
                     region; pfam15255"
                     /db_xref="CDD:464595"
     CDS             1..1496
                     /gene="nclb"
                     /coded_by="XM_076691943.1:356..4846"
                     /db_xref="GeneID:117605737"
ORIGIN      
        1 mnekykemef vdsdseeeed ednkvvlsvv gpnnpyqdrp lpyvigsekw knsskigles
       61 sssseseqvd eeesdseadd taafknlnld pksnlvgllp slndtynkrn ditymdndkm
      121 dvvsqnnids isesvtpnns vpkvslsnds apnfaeelak rlgtvrqaqk ptvmdeksda
      181 sinrfkedlf apeedenvsi dksknilses kkfvndqsse nlwkekpikp yknniipasi
      241 dvpppisais tkpksaiddl fadadsedsd difsskntvk titknkhlsn snvladkena
      301 qkkfldivap thmatstpdt dvnttnlfsd dedmgdlfgs sknqpqnkkk pvggisilgn
      361 ilttdvenkl ssrisrtqss gssgtntptt ygsstnnepv ndrsrnnvsn dnvnerdsie
      421 iarnnavnla vpnesnyssg isnpswsinn tvgssigins pqmtstrlks eeiyrervvs
      481 dslfsaqtrn pisvtpnstn nqsqresnen vlsteqddvf enedlfgppp lpkadsklak
      541 skvsslfdds dsgdelfstt ssgsrsqkss dfftsgvqhp ekakttprkg lfdeeidifg
      601 nkdspdidif giaskspakp ennisswkip disndglfas nakdtivksn tmernqsvgv
      661 tkkislfdde ediddgdlfg tkpvkneaks esrilknddd ekkkkekehk eedelfsakk
      721 qandkssigt tasgtdnveq dvstlssekv dknvlsgnsl fsntigthgl vfedddyddl
      781 fstkstksvi keetkkesvr sksetiddtk etlakeiktf desnifakae epsafdttsn
      841 kveekndkdv hrvssaepsv aedienetkk sppksldiqt vassspseen dqgtkrtvsg
      901 kiknlmgkmg dlkilspmdv pplwrrseek tdeedstadr dsddggcisi qghsslsase
      961 dsatqkqslq stisgesnve saisfdepvq vetlsttask trvriqakrr pqsryarksa
     1021 ltqigidfdt vdasesnsqh dshgnpsqni fnkdpvshtv shtvatssdr lisdnlhrtt
     1081 danifltadd kselgslske ssmsankntl lspstdeedl fdvppdlped pqkedtlfgr
     1141 apilspveqv iservstsfk plkdthikqi ngtdggieen tqkteennvl depsnnsngk
     1201 itvphkseee fkednieakd elndvvdplr dsthdplkdp sqlfafvtkt pspekgknll
     1261 fseddslfss ggkksteeqt tkksvldlfs ddmggdlfst pltkplkkpl kdakislfdd
     1321 deqddeddsl fgpvvkkssv ksesikkpav qqsgkklslf ddddndtnlf sesseqtqks
     1381 dsgsvqeqsn kneifasive pvktshitdi fadqssgedd ifasktvpkk vvtskslfps
     1441 ddddddgdni fgkksttety vkttetrsti kksvtkdlkk taekigddpl silqdd
//
LOCUS       XP_034183287            1629 aa            linear   INV 03-SEP-2025
DEFINITION  PWP1 homolog no child left behind isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183287
VERSION     XP_034183287.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327396.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183287.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1629
                     /product="PWP1 homolog no child left behind isoform X4"
                     /calculated_mol_wt=179369
     Region          <1088..1183
                     /region_name="CAP-ZIP_m"
                     /note="WASH complex subunit CAP-Z interacting, central
                     region; pfam15255"
                     /db_xref="CDD:464595"
     CDS             1..1629
                     /gene="nclb"
                     /coded_by="XM_034327396.2:68..4957"
                     /db_xref="GeneID:117605737"
ORIGIN      
        1 mdisdemdks wdhpwtteem rkkrrewsla gdagllkhlq qfsenlvska nkveetldsl
       61 ttqlhettif idnvtnrsla lantqfiesr vqeddieiek kvetsvqeck dqdlvaaeli
      121 atvsdsikqg lnimnekyke mefvdsdsee eedednkvvl svvgpnnpyq drplpyvigs
      181 ekwknsskig lesssssese qvdeeesdse addtaafknl nldpksnlvg llpslndtyn
      241 krnditymdn dkmdvvsqnn idsisesvtp nnsvpkvsls ndsapnfaee lakrlgtvrq
      301 aqkptvmdek sdasinrfke dlfapeeden vsidksknil seskkfvndq ssenlwkekp
      361 ikpyknniip asidvpppis aistkpksai ddlfadadse dsddifsskn tvktitknkh
      421 lsnsnvladk enaqkkfldi vapthmatst pdtdvnttnl fsddedmgdl fgssknqpqn
      481 kkkpvggisi lgnilttdve nklssrisrt qssgssgtnt pttygsstnn epvndrsrnn
      541 vsndnvnerd sieiarnnav nlavpnesny ssgisnpsws inntvgssig inspqmtstr
      601 lkseeiyrer vvsdslfsaq trnpisvtpn stnnqsqres nenvlsteqd dvfenedlfg
      661 ppplpkadsk lakskvsslf ddsdsgdelf sttssgsrsq kssdfftsgv qhpekakttp
      721 rkglfdeeid ifgnkdspdi difgiasksp akpennissw kipdisndgl fasnakdtiv
      781 ksntmernqs vgvtkkislf ddeediddgd lfgtkpvkne aksesrilkn dddekkkkek
      841 ehkeedelfs akkqandkss igttasgtdn veqdvstlss ekvdknvlsg nslfsntigt
      901 hglvfedddy ddlfstkstk svikeetkke svrsksetid dtketlakei ktfdesnifa
      961 kaeepsafdt tsnkveeknd kdvhrvssae psvaediene tkksppksld iqtvasssps
     1021 eendqgtkrt vsgkiknlmg kmgdlkilsp mdvpplwrrs eektdeedst adrdsddggc
     1081 isiqghssls asedsatqkq slqstisges nvesaisfde pvqvetlstt asktrvriqa
     1141 krrpqsryar ksaltqigid fdtvdasesn sqhdshgnps qnifnkdpvs htvshtvats
     1201 sdrlisdnlh rttdaniflt addkselgsl skessmsank ntllspstde edlfdvppdl
     1261 pedpqkedtl fgrapilspv eqviservst sfkplkdthi kqingtdggi eentqkteen
     1321 nvldepsnns ngkitvphks eeefkednie akdelndvvd plrdsthdpl kdpsqlfafv
     1381 tktpspekgk nllfseddsl fssggkkste eqttkksvld lfsddmggdl fstpltkplk
     1441 kplkdakisl fdddeqdded dslfgpvvkk ssvksesikk pavqqsgkkl slfddddndt
     1501 nlfsesseqt qksdsgsvqe qsnkneifas ivepvktshi tdifadqssg eddifasktv
     1561 pkkvvtsksl fpsddddddg dnifgkkstt etyvkttetr stikksvtkd lkktaekigd
     1621 dplsilqdd
//
LOCUS       XP_034183201             533 aa            linear   INV 03-SEP-2025
DEFINITION  fatty acyl-CoA reductase wat [Osmia lignaria lignaria].
ACCESSION   XP_034183201
VERSION     XP_034183201.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327310.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183201.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="fatty acyl-CoA reductase wat"
                     /calculated_mol_wt=61079
     Region          36..362
                     /region_name="FAR-N_SDR_e"
                     /note="fatty acyl CoA reductases (FARs), extended (e)
                     SDRs; cd05236"
                     /db_xref="CDD:187547"
     Site            order(41,43..46,68..70,134..136,153,174..176,227,231,
                     252..255)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Site            order(154,176,227,231)
                     /site_type="active"
                     /db_xref="CDD:187547"
     Site            order(176,227,254,266,282,288)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Region          391..482
                     /region_name="FAR_C"
                     /note="C-terminal domain of fatty acyl CoA reductases;
                     cd09071"
                     /db_xref="CDD:176924"
     CDS             1..533
                     /gene="LOC117605697"
                     /coded_by="XM_034327310.2:196..1797"
                     /db_xref="GeneID:117605697"
ORIGIN      
        1 mttercdsyv hdildmsepp estekseiip ffdqanilvt ggtgflgkll vekllrccpn
       61 isklylvvrp kkekssseri kenfddiiyd rlkkeqpnfl skvvlvegdg skpefgwspe
      121 ikqmlmntni ifhaaalvrf eaklrvitat nvqtvkfllt fvkqipsfka fvhvstayah
      181 ctrdfidekh ysdtidadkl vslldilddd mitrttpill dkwpntyvft kaigenavlk
      241 ysndlpvciv rpaivtstyt episgwtnnl ygatgvvlga glgllhtlhc ckekvadiip
      301 adyvvatiia aawdvgtrkr pikaspdsii pdeekvpiyn svsscqnpit wgefmkkned
      361 yafevpskkv vwyyalilnn ylfmhnicaf lfhlvpaviv dtaayltgrk pilmdayrki
      421 hkfsnvihyf strewnfrne nvlklwnkmn svdrknfffd igkldwdayf yihvrglrvf
      481 mlndpldtld esrvaysrlr kihytlltsm illslwilys fisylwsfcp lah
//
LOCUS       XP_034183222              90 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605704 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183222
VERSION     XP_034183222.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327331.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183222.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..90
                     /product="uncharacterized protein LOC117605704 isoform X1"
                     /calculated_mol_wt=9596
     Region          <41..88
                     /region_name="SVWC"
                     /note="Single domain von Willebrand factor type C;
                     pfam15430"
                     /db_xref="CDD:464713"
     CDS             1..90
                     /gene="LOC117605704"
                     /coded_by="XM_034327331.2:81..353"
                     /db_xref="GeneID:117605704"
ORIGIN      
        1 mktiilvsll mamcllsevr agtckgygdk dlnvgthyin ctavschpng svtmlgcasy
       61 rcqegkqigy rqtdlskpyp eccegpicad
//
LOCUS       XP_034183223              89 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605704 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183223
VERSION     XP_034183223.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327332.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183223.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..89
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..89
                     /product="uncharacterized protein LOC117605704 isoform X2"
                     /calculated_mol_wt=9525
     Region          <40..87
                     /region_name="SVWC"
                     /note="Single domain von Willebrand factor type C;
                     pfam15430"
                     /db_xref="CDD:464713"
     CDS             1..89
                     /gene="LOC117605704"
                     /coded_by="XM_034327332.2:100..369"
                     /db_xref="GeneID:117605704"
ORIGIN      
        1 mktiilvsll mamcllsevr gtckgygdkd lnvgthyinc tavschpngs vtmlgcasyr
       61 cqegkqigyr qtdlskpype ccegpicad
//
LOCUS       XP_034190713             480 aa            linear   INV 03-SEP-2025
DEFINITION  aromatic-L-amino-acid decarboxylase [Osmia lignaria lignaria].
ACCESSION   XP_034190713
VERSION     XP_034190713.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334822.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..480
                     /product="aromatic-L-amino-acid decarboxylase"
                     /calculated_mol_wt=54184
     Region          35..412
                     /region_name="Pyridoxal_deC"
                     /note="Pyridoxal-dependent decarboxylase conserved domain;
                     pfam00282"
                     /db_xref="CDD:395219"
     Site            order(148..149,152,242,271,274,300,303)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99743"
     Site            303
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99743"
     CDS             1..480
                     /gene="Ddc"
                     /coded_by="XM_034334822.2:189..1631"
                     /db_xref="GeneID:117609005"
ORIGIN      
        1 mdpdgfkdfa kemaeyivny lenirdrkvl ptvepgymkp llpaeapqtp eswkdimadi
       61 ervimpgvth whspkfhayf ptaqsypaiv admlsgaiac igftwiaspa ctelevimld
      121 wlgkmldlpn eflacsggkg ggviqgtase atlvallgak arkirqvkeq hpewtdneiv
      181 gklvaysscq ahssveragl lggvkfrsle vdskyklrge tlaeairkdr eqglipfyav
      241 atlgttcsca fdrldemgvv anreniwlhv daayagsafi cpefrhlmng ieladsfnfn
      301 phkwmlvnfd cstmwlkdpt yvinafnvdp lylkhdmqgs apdyrhwqip lgrrfralkl
      361 wfvlriygve nlqryirthv aqahefealv lsdprfeiva evvlglvcfr lkgsndlnen
      421 llkkinatkn ihlvpskind tyflrfaics rfseskdiqn swkeiklrae evleeqsvsk
//
LOCUS       XP_034186387             620 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607164 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186387
VERSION     XP_034186387.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..620
                     /product="uncharacterized protein LOC117607164 isoform X1"
                     /calculated_mol_wt=69142
     Region          282..611
                     /region_name="MtnN"
                     /note="Nucleoside phosphorylase/nucleosidase, includes
                     5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
                     MtnN and futalosine hydrolase MqnB [Nucleotide transport
                     and metabolism, Coenzyme transport and metabolism];
                     COG0775"
                     /db_xref="CDD:440538"
     CDS             1..620
                     /gene="LOC117607164"
                     /coded_by="XM_034330496.2:528..2390"
                     /db_xref="GeneID:117607164"
ORIGIN      
        1 mtemdnkete hivmtpkckt ansttliier qfleppakne nennvhiagg dhkgivinkq
       61 aasvtngeih pahlclqfrv flvsaqtgkh tqesrtlqfw fsgtlleseh psvaqeffre
      121 lvspqefprd yvgfikkimk lmlhkystik kievelrqle epitlparps dtghiqltlp
      181 ykkiiwksvs tdetvmgqvi eltlekvlel iesaypnpvt vtdmakehgw dpsaveiklk
      241 elqekgvvka mehgaftrvv hqdtqvqvvk qmptmasakq ptiaiitaqy ceklavdsmi
      301 enketfvryt tvgtasspda tdgvprvicr fgesnvytlg nigahrivct klptvghtre
      361 amtaagnttt rllgtfqkvd fvfligiggg vphytdynkh vrlgdvvvsy papvnkkyiy
      421 aycesakmne sgdyhfetke ycppnlylqe iaanlrdqse nepnppwqky lkegldalsn
      481 qtehdfkapp pesdklymai gerdvievah ptapsdavnk rvdgcsrihl apvasgrhia
      541 rddqlrqkfa arfralafda emdavvesil gncresfavi rgisdykdgs rikewqpfas
      601 laaasvmksi icamdpptnv
//
LOCUS       XP_076548059             601 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607164 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548059
VERSION     XP_076548059.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691944.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..601
                     /product="uncharacterized protein LOC117607164 isoform X2"
                     /calculated_mol_wt=66946
     Region          263..592
                     /region_name="MtnN"
                     /note="Nucleoside phosphorylase/nucleosidase, includes
                     5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
                     MtnN and futalosine hydrolase MqnB [Nucleotide transport
                     and metabolism, Coenzyme transport and metabolism];
                     COG0775"
                     /db_xref="CDD:440538"
     CDS             1..601
                     /gene="LOC117607164"
                     /coded_by="XM_076691944.1:472..2277"
                     /db_xref="GeneID:117607164"
ORIGIN      
        1 mtemdnkete hivmtpkckt ansttliier qfleppakne nennvhiagg dhkgivinkq
       61 aasvtngeih pahlclqfrv flvsaqtgkh tqesrtlqfw fsgtlleseh psvaqeffre
      121 lvspqefprd yvgfikkimk lmlhkystik kievelrqle epitlparpl stdetvmgqv
      181 ieltlekvle liesaypnpv tvtdmakehg wdpsaveikl kelqekgvvk amehgaftrv
      241 vhqdtqvqvv kqmptmasak qptiaiitaq yceklavdsm ienketfvry ttvgtasspd
      301 atdgvprvic rfgesnvytl gnigahrivc tklptvghtr eamtaagntt trllgtfqkv
      361 dfvfligigg gvphytdynk hvrlgdvvvs ypapvnkkyi yaycesakmn esgdyhfetk
      421 eycppnlylq eiaanlrdqs enepnppwqk ylkegldals nqtehdfkap ppesdklyma
      481 igerdvieva hptapsdavn krvdgcsrih lapvasgrhi arddqlrqkf aarfralafd
      541 aemdavvesi lgncresfav irgisdykdg srikewqpfa slaaasvmks iicamdpptn
      601 v
//
LOCUS       XP_076548060             601 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607164 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548060
VERSION     XP_076548060.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691945.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..601
                     /product="uncharacterized protein LOC117607164 isoform X3"
                     /calculated_mol_wt=67211
     Region          282..592
                     /region_name="MtnN"
                     /note="Nucleoside phosphorylase/nucleosidase, includes
                     5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
                     MtnN and futalosine hydrolase MqnB [Nucleotide transport
                     and metabolism, Coenzyme transport and metabolism];
                     COG0775"
                     /db_xref="CDD:440538"
     CDS             1..601
                     /gene="LOC117607164"
                     /coded_by="XM_076691945.1:476..2281"
                     /db_xref="GeneID:117607164"
ORIGIN      
        1 mtemdnkete hivmtpkckt ansttliier qfleppakne nennvhiagg dhkgivinkq
       61 aasvtngeih pahlclqfrv flvsaqtgkh tqesrtlqfw fsgtlleseh psvaqeffre
      121 lvspqefprd yvgfikkimk lmlhkystik kievelrqle epitlparps dtghiqltlp
      181 ykkiiwksvs tdetvmgqvi eltlekvlel iesaypnpvt vtdmakehgw dpsaveiklk
      241 elqekgvvka mehgaftrvv hqdtqvqvvk qmptmasakq ptiaiitaqy ceklavdsmi
      301 enketfvryt tvgesnvytl gnigahrivc tklptvghtr eamtaagntt trllgtfqkv
      361 dfvfligigg gvphytdynk hvrlgdvvvs ypapvnkkyi yaycesakmn esgdyhfetk
      421 eycppnlylq eiaanlrdqs enepnppwqk ylkegldals nqtehdfkap ppesdklyma
      481 igerdvieva hptapsdavn krvdgcsrih lapvasgrhi arddqlrqkf aarfralafd
      541 aemdavvesi lgncresfav irgisdykdg srikewqpfa slaaasvmks iicamdpptn
      601 v
//
LOCUS       XP_034186388             582 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607164 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186388
VERSION     XP_034186388.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330497.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..582
                     /product="uncharacterized protein LOC117607164 isoform X4"
                     /calculated_mol_wt=65015
     Region          263..573
                     /region_name="MtnN"
                     /note="Nucleoside phosphorylase/nucleosidase, includes
                     5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
                     MtnN and futalosine hydrolase MqnB [Nucleotide transport
                     and metabolism, Coenzyme transport and metabolism];
                     COG0775"
                     /db_xref="CDD:440538"
     CDS             1..582
                     /gene="LOC117607164"
                     /coded_by="XM_034330497.2:469..2217"
                     /db_xref="GeneID:117607164"
ORIGIN      
        1 mtemdnkete hivmtpkckt ansttliier qfleppakne nennvhiagg dhkgivinkq
       61 aasvtngeih pahlclqfrv flvsaqtgkh tqesrtlqfw fsgtlleseh psvaqeffre
      121 lvspqefprd yvgfikkimk lmlhkystik kievelrqle epitlparpl stdetvmgqv
      181 ieltlekvle liesaypnpv tvtdmakehg wdpsaveikl kelqekgvvk amehgaftrv
      241 vhqdtqvqvv kqmptmasak qptiaiitaq yceklavdsm ienketfvry ttvgesnvyt
      301 lgnigahriv ctklptvght reamtaagnt ttrllgtfqk vdfvfligig ggvphytdyn
      361 khvrlgdvvv sypapvnkky iyaycesakm nesgdyhfet keycppnlyl qeiaanlrdq
      421 senepnppwq kylkegldal snqtehdfka pppesdklym aigerdviev ahptapsdav
      481 nkrvdgcsri hlapvasgrh iarddqlrqk faarfralaf daemdavves ilgncresfa
      541 virgisdykd gsrikewqpf aslaaasvmk siicamdppt nv
//
LOCUS       XP_034186389             526 aa            linear   INV 03-SEP-2025
DEFINITION  sialin-like [Osmia lignaria lignaria].
ACCESSION   XP_034186389
VERSION     XP_034186389.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186389.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..526
                     /product="sialin-like"
                     /calculated_mol_wt=59437
     Region          23..475
                     /region_name="MFS_SLC17"
                     /note="Solute carrier 17 (SLC17) family of the Major
                     Facilitator Superfamily of transporters; cd17318"
                     /db_xref="CDD:340876"
     Site            order(33..34,37..38,41,119,171..172,174..176,179,198,
                     201..202,205,294,297..298,301..303,306,334,338,397..398,
                     402,406,422,425..426,429..430,433)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340876"
     CDS             1..526
                     /gene="LOC117607165"
                     /coded_by="XM_034330498.2:294..1874"
                     /db_xref="GeneID:117607165"
ORIGIN      
        1 meqlknekmk nrrelitcrd vlwylvffgf aanymmrinl nltivamvlp rpkttiiaqc
       61 nsesiqllhn kthdinstft tpstipipyn vtyedrfewn eyqqglalga yywlhwlsql
      121 pggllarryg tklvyglgnf ftaligffip fithyhlyal vflrvlqgla agviwpsmhn
      181 mtakwippne rsrfvsaylg ssvgaavtyp lcaavsntfg waaafyvtsl vgvvwyvfwl
      241 flvydspqqh prisdkekky ildnlgdsvd eqetripwks iltsrpvwvt iaanwssgwg
      301 fltlmtqaps yfnfihgwni navgmisgap hlmrmifsyy fsimsdwlir tkrmsltnvr
      361 klatfvstgl lgvlililgf sgcqptlaii fmmagiting avsassfanf vdlspnyasv
      421 llglcglvvi wsgfispavv gvltnnnqti sqwrsvfiia ainsfagtii fllfgtskeq
      481 rwnkygkvde kmeqemqklt kataikceeg nekmdvnekd kmieqk
//
LOCUS       XP_034186390             433 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607166 [Osmia lignaria lignaria].
ACCESSION   XP_034186390
VERSION     XP_034186390.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330499.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..433
                     /product="uncharacterized protein LOC117607166"
                     /calculated_mol_wt=49340
     Region          2..393
                     /region_name="PLN02172"
                     /note="flavin-containing monooxygenase FMO GS-OX"
                     /db_xref="CDD:215116"
     CDS             1..433
                     /gene="LOC117607166"
                     /coded_by="XM_034330499.2:377..1678"
                     /db_xref="GeneID:117607166"
ORIGIN      
        1 mpssktriai igggvaglvv arhtaakldt ysltlfeqtd qiggtwvytd etdvdkhglp
       61 vhssmyknlr tnlpreimqi pdfplkydeg ssfvhhsvir eylgdyakhf nlyphiklnt
      121 lvkhvepetl pndqviwmvt yqdleskvet tktfdavvlc nghytvghip lipgiesfpg
      181 dsihshqyrv pdvyakknvc ilgaswsgid ialevsqyad kvylshnlpd pvdsrmsrnv
      241 eqrvgiqsiq gnifvfrdgs taevdsfiyc tgyeftypfm sskveirtdn nhvepvykhl
      301 ihtdypnlfv mglpgivipf pmfhlqaqyi lgilegrvql psseqmrqey eaekkalldq
      361 giplrhinkl merqwayyde iataanipgf ppvikkiydh vsqmreldit iyknyqyrii
      421 ddenfvvccc kpc
//
LOCUS       XP_034186345             713 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034186345
VERSION     XP_034186345.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330454.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..713
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X1"
                     /calculated_mol_wt=79965
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <341..659
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          342..387
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          444..483
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          493..532
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          539..580
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          586..626
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..713
                     /gene="sw"
                     /coded_by="XM_034330454.2:174..2315"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kggsapnyls hafdyydeyn lnpglewede ftaedeensl phmdgfqskl ppgilphglp
      241 qvkevqpavt qveqekekek pkevrelsee ekqmiilsed fqrfldrtsr iveralgesv
      301 diytdytgtm dgedgmdeks hqrlwlnrsf fcdrwsrnrc vtsmdwspqf pellaasynn
      361 nddipndpdg vclvwntkfk kttpefifhc qspvmsttfa rfhpnlilgg tysgqivlwd
      421 nrvqkrspiq rtplsasaht hpvyclnivg aqnahnlisi stdgklcsws lemlsqplet
      481 lelhtkqskp iaatclafpy gdvnnfvvgs edgtvysacr hgtkagvlet yeghqgpvtg
      541 isahavqggi dfshlfltss idwtiklwsl kenkplysfe hngdyvydva wspthpalfa
      601 avddsgrldl wnlnqdtevp tasvlidgcp alnrvswtps glhvtvgddt gkiwvydvae
      661 hlahpridew nkflytqqel khnkadeelh klnlrepasl tsihpllttg plr
//
LOCUS       XP_034186346             712 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034186346
VERSION     XP_034186346.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330455.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..712
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X2"
                     /calculated_mol_wt=79877
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <340..658
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          341..386
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          443..482
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          492..531
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          538..579
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          585..625
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..712
                     /gene="sw"
                     /coded_by="XM_034330455.2:174..2312"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdglsass saytiyscst tpthsysagy feadwwrprk
      181 ggsapnylsh afdyydeynl npglewedef taedeenslp hmdgfqsklp pgilphglpq
      241 vkevqpavtq veqekekekp kevrelseee kqmiilsedf qrfldrtsri veralgesvd
      301 iytdytgtmd gedgmdeksh qrlwlnrsff cdrwsrnrcv tsmdwspqfp ellaasynnn
      361 ddipndpdgv clvwntkfkk ttpefifhcq spvmsttfar fhpnlilggt ysgqivlwdn
      421 rvqkrspiqr tplsasahth pvyclnivga qnahnlisis tdgklcswsl emlsqpletl
      481 elhtkqskpi aatclafpyg dvnnfvvgse dgtvysacrh gtkagvlety eghqgpvtgi
      541 sahavqggid fshlfltssi dwtiklwslk enkplysfeh ngdyvydvaw spthpalfaa
      601 vddsgrldlw nlnqdtevpt asvlidgcpa lnrvswtpsg lhvtvgddtg kiwvydvaeh
      661 lahpridewn kflytqqelk hnkadeelhk lnlrepaslt sihpllttgp lr
//
LOCUS       XP_034186347             706 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034186347
VERSION     XP_034186347.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330456.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..706
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X3"
                     /calculated_mol_wt=79129
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <334..652
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          335..380
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          437..476
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          486..525
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          532..573
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          579..619
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..706
                     /gene="sw"
                     /coded_by="XM_034330456.2:174..2294"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kggsapnyly eynlnpglew edeftaedee nslphmdgfq sklppgilph glpqvkevqp
      241 avtqveqeke kekpkevrel seeekqmiil sedfqrfldr tsriveralg esvdiytdyt
      301 gtmdgedgmd ekshqrlwln rsffcdrwsr nrcvtsmdws pqfpellaas ynnnddipnd
      361 pdgvclvwnt kfkkttpefi fhcqspvmst tfarfhpnli lggtysgqiv lwdnrvqkrs
      421 piqrtplsas ahthpvycln ivgaqnahnl isistdgklc swslemlsqp letlelhtkq
      481 skpiaatcla fpygdvnnfv vgsedgtvys acrhgtkagv letyeghqgp vtgisahavq
      541 ggidfshlfl tssidwtikl wslkenkply sfehngdyvy dvawspthpa lfaavddsgr
      601 ldlwnlnqdt evptasvlid gcpalnrvsw tpsglhvtvg ddtgkiwvyd vaehlahpri
      661 dewnkflytq qelkhnkade elhklnlrep asltsihpll ttgplr
//
LOCUS       XP_034186348             705 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034186348
VERSION     XP_034186348.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330457.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..705
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X4"
                     /calculated_mol_wt=78887
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <333..651
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          334..379
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          436..475
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          485..524
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          531..572
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          578..618
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..705
                     /gene="sw"
                     /coded_by="XM_034330457.2:174..2291"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kggsapnyls hafdyyvltf ddgqaedeen slphmdgfqs klppgilphg lpqvkevqpa
      241 vtqveqekek ekpkevrels eeekqmiils edfqrfldrt sriveralge svdiytdytg
      301 tmdgedgmde kshqrlwlnr sffcdrwsrn rcvtsmdwsp qfpellaasy nnnddipndp
      361 dgvclvwntk fkkttpefif hcqspvmstt farfhpnlil ggtysgqivl wdnrvqkrsp
      421 iqrtplsasa hthpvyclni vgaqnahnli sistdgklcs wslemlsqpl etlelhtkqs
      481 kpiaatclaf pygdvnnfvv gsedgtvysa crhgtkagvl etyeghqgpv tgisahavqg
      541 gidfshlflt ssidwtiklw slkenkplys fehngdyvyd vawspthpal faavddsgrl
      601 dlwnlnqdte vptasvlidg cpalnrvswt psglhvtvgd dtgkiwvydv aehlahprid
      661 ewnkflytqq elkhnkadee lhklnlrepa sltsihpllt tgplr
//
LOCUS       XP_034186349             705 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_034186349
VERSION     XP_034186349.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330458.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..705
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X5"
                     /calculated_mol_wt=79189
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <333..651
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          334..379
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          436..475
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          485..524
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          531..572
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          578..618
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..705
                     /gene="sw"
                     /coded_by="XM_034330458.2:174..2291"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kahafdyyde ynlnpglewe deftaedeen slphmdgfqs klppgilphg lpqvkevqpa
      241 vtqveqekek ekpkevrels eeekqmiils edfqrfldrt sriveralge svdiytdytg
      301 tmdgedgmde kshqrlwlnr sffcdrwsrn rcvtsmdwsp qfpellaasy nnnddipndp
      361 dgvclvwntk fkkttpefif hcqspvmstt farfhpnlil ggtysgqivl wdnrvqkrsp
      421 iqrtplsasa hthpvyclni vgaqnahnli sistdgklcs wslemlsqpl etlelhtkqs
      481 kpiaatclaf pygdvnnfvv gsedgtvysa crhgtkagvl etyeghqgpv tgisahavqg
      541 gidfshlflt ssidwtiklw slkenkplys fehngdyvyd vawspthpal faavddsgrl
      601 dlwnlnqdte vptasvlidg cpalnrvswt psglhvtvgd dtgkiwvydv aehlahprid
      661 ewnkflytqq elkhnkadee lhklnlrepa sltsihpllt tgplr
//
LOCUS       XP_076548061             704 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X6
            [Osmia lignaria lignaria].
ACCESSION   XP_076548061
VERSION     XP_076548061.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691946.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..704
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X6"
                     /calculated_mol_wt=78800
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <332..650
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          333..378
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          435..474
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          484..523
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          530..571
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          577..617
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..704
                     /gene="sw"
                     /coded_by="XM_076691946.1:174..2288"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdglsass saytiyscst tpthsysagy feadwwrprk
      181 ggsapnylsh afdyyvltfd dgqaedeens lphmdgfqsk lppgilphgl pqvkevqpav
      241 tqveqekeke kpkevrelse eekqmiilse dfqrfldrts riveralges vdiytdytgt
      301 mdgedgmdek shqrlwlnrs ffcdrwsrnr cvtsmdwspq fpellaasyn nnddipndpd
      361 gvclvwntkf kkttpefifh cqspvmsttf arfhpnlilg gtysgqivlw dnrvqkrspi
      421 qrtplsasah thpvyclniv gaqnahnlis istdgklcsw slemlsqple tlelhtkqsk
      481 piaatclafp ygdvnnfvvg sedgtvysac rhgtkagvle tyeghqgpvt gisahavqgg
      541 idfshlflts sidwtiklws lkenkplysf ehngdyvydv awspthpalf aavddsgrld
      601 lwnlnqdtev ptasvlidgc palnrvswtp sglhvtvgdd tgkiwvydva ehlahpride
      661 wnkflytqqe lkhnkadeel hklnlrepas ltsihplltt gplr
//
LOCUS       XP_034186350             698 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X7
            [Osmia lignaria lignaria].
ACCESSION   XP_034186350
VERSION     XP_034186350.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330459.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..698
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X7"
                     /calculated_mol_wt=78321
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <326..644
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          327..372
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          429..468
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          478..517
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          524..565
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          571..611
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..698
                     /gene="sw"
                     /coded_by="XM_034330459.2:174..2270"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kdeynlnpgl ewedeftaed eenslphmdg fqsklppgil phglpqvkev qpavtqveqe
      241 kekekpkevr elseeekqmi ilsedfqrfl drtsrivera lgesvdiytd ytgtmdgedg
      301 mdekshqrlw lnrsffcdrw srnrcvtsmd wspqfpella asynnnddip ndpdgvclvw
      361 ntkfkkttpe fifhcqspvm sttfarfhpn lilggtysgq ivlwdnrvqk rspiqrtpls
      421 asahthpvyc lnivgaqnah nlisistdgk lcswslemls qpletlelht kqskpiaatc
      481 lafpygdvnn fvvgsedgtv ysacrhgtka gvletyeghq gpvtgisaha vqggidfshl
      541 fltssidwti klwslkenkp lysfehngdy vydvawspth palfaavdds grldlwnlnq
      601 dtevptasvl idgcpalnrv swtpsglhvt vgddtgkiwv ydvaehlahp ridewnkfly
      661 tqqelkhnka deelhklnlr epasltsihp llttgplr
//
LOCUS       XP_034186352             698 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X8
            [Osmia lignaria lignaria].
ACCESSION   XP_034186352
VERSION     XP_034186352.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330461.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..698
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X8"
                     /calculated_mol_wt=78052
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <326..644
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          327..372
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          429..468
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          478..517
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          524..565
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          571..611
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..698
                     /gene="sw"
                     /coded_by="XM_034330461.2:174..2270"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kggsapnylf ltfddgqaed eenslphmdg fqsklppgil phglpqvkev qpavtqveqe
      241 kekekpkevr elseeekqmi ilsedfqrfl drtsrivera lgesvdiytd ytgtmdgedg
      301 mdekshqrlw lnrsffcdrw srnrcvtsmd wspqfpella asynnnddip ndpdgvclvw
      361 ntkfkkttpe fifhcqspvm sttfarfhpn lilggtysgq ivlwdnrvqk rspiqrtpls
      421 asahthpvyc lnivgaqnah nlisistdgk lcswslemls qpletlelht kqskpiaatc
      481 lafpygdvnn fvvgsedgtv ysacrhgtka gvletyeghq gpvtgisaha vqggidfshl
      541 fltssidwti klwslkenkp lysfehngdy vydvawspth palfaavdds grldlwnlnq
      601 dtevptasvl idgcpalnrv swtpsglhvt vgddtgkiwv ydvaehlahp ridewnkfly
      661 tqqelkhnka deelhklnlr epasltsihp llttgplr
//
LOCUS       XP_034186353             697 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X9
            [Osmia lignaria lignaria].
ACCESSION   XP_034186353
VERSION     XP_034186353.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330462.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X9"
                     /calculated_mol_wt=78112
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <325..643
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          326..371
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          428..467
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          477..516
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          523..564
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          570..610
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..697
                     /gene="sw"
                     /coded_by="XM_034330462.2:174..2267"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kahafdyyvl tfddgqaede enslphmdgf qsklppgilp hglpqvkevq pavtqveqek
      241 ekekpkevre lseeekqmii lsedfqrfld rtsriveral gesvdiytdy tgtmdgedgm
      301 dekshqrlwl nrsffcdrws rnrcvtsmdw spqfpellaa synnnddipn dpdgvclvwn
      361 tkfkkttpef ifhcqspvms ttfarfhpnl ilggtysgqi vlwdnrvqkr spiqrtplsa
      421 sahthpvycl nivgaqnahn lisistdgkl cswslemlsq pletlelhtk qskpiaatcl
      481 afpygdvnnf vvgsedgtvy sacrhgtkag vletyeghqg pvtgisahav qggidfshlf
      541 ltssidwtik lwslkenkpl ysfehngdyv ydvawspthp alfaavddsg rldlwnlnqd
      601 tevptasvli dgcpalnrvs wtpsglhvtv gddtgkiwvy dvaehlahpr idewnkflyt
      661 qqelkhnkad eelhklnlre pasltsihpl lttgplr
//
LOCUS       XP_034186354             697 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X10
            [Osmia lignaria lignaria].
ACCESSION   XP_034186354
VERSION     XP_034186354.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..697
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X10"
                     /calculated_mol_wt=78012
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <325..643
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          326..371
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          428..467
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          477..516
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          523..564
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          570..610
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..697
                     /gene="sw"
                     /coded_by="XM_034330463.2:174..2267"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kggsapnyls hafdyyaede enslphmdgf qsklppgilp hglpqvkevq pavtqveqek
      241 ekekpkevre lseeekqmii lsedfqrfld rtsriveral gesvdiytdy tgtmdgedgm
      301 dekshqrlwl nrsffcdrws rnrcvtsmdw spqfpellaa synnnddipn dpdgvclvwn
      361 tkfkkttpef ifhcqspvms ttfarfhpnl ilggtysgqi vlwdnrvqkr spiqrtplsa
      421 sahthpvycl nivgaqnahn lisistdgkl cswslemlsq pletlelhtk qskpiaatcl
      481 afpygdvnnf vvgsedgtvy sacrhgtkag vletyeghqg pvtgisahav qggidfshlf
      541 ltssidwtik lwslkenkpl ysfehngdyv ydvawspthp alfaavddsg rldlwnlnqd
      601 tevptasvli dgcpalnrvs wtpsglhvtv gddtgkiwvy dvaehlahpr idewnkflyt
      661 qqelkhnkad eelhklnlre pasltsihpl lttgplr
//
LOCUS       XP_034186355             690 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X11
            [Osmia lignaria lignaria].
ACCESSION   XP_034186355
VERSION     XP_034186355.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330464.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..690
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X11"
                     /calculated_mol_wt=77244
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <318..636
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          319..364
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          421..460
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          470..509
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          516..557
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          563..603
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..690
                     /gene="sw"
                     /coded_by="XM_034330464.2:174..2246"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kvltfddgqa edeenslphm dgfqsklppg ilphglpqvk evqpavtqve qekekekpke
      241 vrelseeekq miilsedfqr fldrtsrive ralgesvdiy tdytgtmdge dgmdekshqr
      301 lwlnrsffcd rwsrnrcvts mdwspqfpel laasynnndd ipndpdgvcl vwntkfkktt
      361 pefifhcqsp vmsttfarfh pnlilggtys gqivlwdnrv qkrspiqrtp lsasahthpv
      421 yclnivgaqn ahnlisistd gklcswslem lsqpletlel htkqskpiaa tclafpygdv
      481 nnfvvgsedg tvysacrhgt kagvletyeg hqgpvtgisa havqggidfs hlfltssidw
      541 tiklwslken kplysfehng dyvydvawsp thpalfaavd dsgrldlwnl nqdtevptas
      601 vlidgcpaln rvswtpsglh vtvgddtgki wvydvaehla hpridewnkf lytqqelkhn
      661 kadeelhkln lrepasltsi hpllttgplr
//
LOCUS       XP_034186356             690 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X12
            [Osmia lignaria lignaria].
ACCESSION   XP_034186356
VERSION     XP_034186356.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330465.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..690
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X12"
                     /calculated_mol_wt=77144
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <318..636
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          319..364
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          421..460
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          470..509
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          516..557
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          563..603
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..690
                     /gene="sw"
                     /coded_by="XM_034330465.2:174..2246"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kggsapnyls edeenslphm dgfqsklppg ilphglpqvk evqpavtqve qekekekpke
      241 vrelseeekq miilsedfqr fldrtsrive ralgesvdiy tdytgtmdge dgmdekshqr
      301 lwlnrsffcd rwsrnrcvts mdwspqfpel laasynnndd ipndpdgvcl vwntkfkktt
      361 pefifhcqsp vmsttfarfh pnlilggtys gqivlwdnrv qkrspiqrtp lsasahthpv
      421 yclnivgaqn ahnlisistd gklcswslem lsqpletlel htkqskpiaa tclafpygdv
      481 nnfvvgsedg tvysacrhgt kagvletyeg hqgpvtgisa havqggidfs hlfltssidw
      541 tiklwslken kplysfehng dyvydvawsp thpalfaavd dsgrldlwnl nqdtevptas
      601 vlidgcpaln rvswtpsglh vtvgddtgki wvydvaehla hpridewnkf lytqqelkhn
      661 kadeelhkln lrepasltsi hpllttgplr
//
LOCUS       XP_034186357             689 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X13
            [Osmia lignaria lignaria].
ACCESSION   XP_034186357
VERSION     XP_034186357.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330466.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..689
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X13"
                     /calculated_mol_wt=77540
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <317..635
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          318..363
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          420..459
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          469..508
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          515..556
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          562..602
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..689
                     /gene="sw"
                     /coded_by="XM_034330466.2:174..2243"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkggs apnylshafd yydeynlnpg
      181 lewedeftae deenslphmd gfqsklppgi lphglpqvke vqpavtqveq ekekekpkev
      241 relseeekqm iilsedfqrf ldrtsriver algesvdiyt dytgtmdged gmdekshqrl
      301 wlnrsffcdr wsrnrcvtsm dwspqfpell aasynnnddi pndpdgvclv wntkfkkttp
      361 efifhcqspv msttfarfhp nlilggtysg qivlwdnrvq krspiqrtpl sasahthpvy
      421 clnivgaqna hnlisistdg klcswsleml sqpletlelh tkqskpiaat clafpygdvn
      481 nfvvgsedgt vysacrhgtk agvletyegh qgpvtgisah avqggidfsh lfltssidwt
      541 iklwslkenk plysfehngd yvydvawspt hpalfaavdd sgrldlwnln qdtevptasv
      601 lidgcpalnr vswtpsglhv tvgddtgkiw vydvaehlah pridewnkfl ytqqelkhnk
      661 adeelhklnl repasltsih pllttgplr
//
LOCUS       XP_034186358             689 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X14
            [Osmia lignaria lignaria].
ACCESSION   XP_034186358
VERSION     XP_034186358.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330467.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..689
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X14"
                     /calculated_mol_wt=77236
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <317..635
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          318..363
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          420..459
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          469..508
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          515..556
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          562..602
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..689
                     /gene="sw"
                     /coded_by="XM_034330467.2:174..2243"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kahafdyyae deenslphmd gfqsklppgi lphglpqvke vqpavtqveq ekekekpkev
      241 relseeekqm iilsedfqrf ldrtsriver algesvdiyt dytgtmdged gmdekshqrl
      301 wlnrsffcdr wsrnrcvtsm dwspqfpell aasynnnddi pndpdgvclv wntkfkkttp
      361 efifhcqspv msttfarfhp nlilggtysg qivlwdnrvq krspiqrtpl sasahthpvy
      421 clnivgaqna hnlisistdg klcswsleml sqpletlelh tkqskpiaat clafpygdvn
      481 nfvvgsedgt vysacrhgtk agvletyegh qgpvtgisah avqggidfsh lfltssidwt
      541 iklwslkenk plysfehngd yvydvawspt hpalfaavdd sgrldlwnln qdtevptasv
      601 lidgcpalnr vswtpsglhv tvgddtgkiw vydvaehlah pridewnkfl ytqqelkhnk
      661 adeelhklnl repasltsih pllttgplr
//
LOCUS       XP_034186359             688 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X15
            [Osmia lignaria lignaria].
ACCESSION   XP_034186359
VERSION     XP_034186359.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330468.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..688
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X15"
                     /calculated_mol_wt=77453
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <316..634
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          317..362
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          419..458
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          468..507
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          514..555
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          561..601
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..688
                     /gene="sw"
                     /coded_by="XM_034330468.2:174..2240"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdgyfead wwrprkggsa pnylshafdy ydeynlnpgl
      181 ewedeftaed eenslphmdg fqsklppgil phglpqvkev qpavtqveqe kekekpkevr
      241 elseeekqmi ilsedfqrfl drtsrivera lgesvdiytd ytgtmdgedg mdekshqrlw
      301 lnrsffcdrw srnrcvtsmd wspqfpella asynnnddip ndpdgvclvw ntkfkkttpe
      361 fifhcqspvm sttfarfhpn lilggtysgq ivlwdnrvqk rspiqrtpls asahthpvyc
      421 lnivgaqnah nlisistdgk lcswslemls qpletlelht kqskpiaatc lafpygdvnn
      481 fvvgsedgtv ysacrhgtka gvletyeghq gpvtgisaha vqggidfshl fltssidwti
      541 klwslkenkp lysfehngdy vydvawspth palfaavdds grldlwnlnq dtevptasvl
      601 idgcpalnrv swtpsglhvt vgddtgkiwv ydvaehlahp ridewnkfly tqqelkhnka
      661 deelhklnlr epasltsihp llttgplr
//
LOCUS       XP_034186360             682 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X16
            [Osmia lignaria lignaria].
ACCESSION   XP_034186360
VERSION     XP_034186360.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330469.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..682
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X16"
                     /calculated_mol_wt=76704
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <310..628
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          311..356
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          413..452
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          462..501
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          508..549
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          555..595
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..682
                     /gene="sw"
                     /coded_by="XM_034330469.2:173..2221"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkggs apnylyeynl npglewedef
      181 taedeenslp hmdgfqsklp pgilphglpq vkevqpavtq veqekekekp kevrelseee
      241 kqmiilsedf qrfldrtsri veralgesvd iytdytgtmd gedgmdeksh qrlwlnrsff
      301 cdrwsrnrcv tsmdwspqfp ellaasynnn ddipndpdgv clvwntkfkk ttpefifhcq
      361 spvmsttfar fhpnlilggt ysgqivlwdn rvqkrspiqr tplsasahth pvyclnivga
      421 qnahnlisis tdgklcswsl emlsqpletl elhtkqskpi aatclafpyg dvnnfvvgse
      481 dgtvysacrh gtkagvlety eghqgpvtgi sahavqggid fshlfltssi dwtiklwslk
      541 enkplysfeh ngdyvydvaw spthpalfaa vddsgrldlw nlnqdtevpt asvlidgcpa
      601 lnrvswtpsg lhvtvgddtg kiwvydvaeh lahpridewn kflytqqelk hnkadeelhk
      661 lnlrepaslt sihpllttgp lr
//
LOCUS       XP_034186361             682 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X17
            [Osmia lignaria lignaria].
ACCESSION   XP_034186361
VERSION     XP_034186361.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330470.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..682
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X17"
                     /calculated_mol_wt=76368
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <310..628
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          311..356
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          413..452
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          462..501
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          508..549
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          555..595
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..682
                     /gene="sw"
                     /coded_by="XM_034330470.2:174..2222"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdglsas ssaytiyscs ttpthsysag yfeadwwrpr
      181 kaedeenslp hmdgfqsklp pgilphglpq vkevqpavtq veqekekekp kevrelseee
      241 kqmiilsedf qrfldrtsri veralgesvd iytdytgtmd gedgmdeksh qrlwlnrsff
      301 cdrwsrnrcv tsmdwspqfp ellaasynnn ddipndpdgv clvwntkfkk ttpefifhcq
      361 spvmsttfar fhpnlilggt ysgqivlwdn rvqkrspiqr tplsasahth pvyclnivga
      421 qnahnlisis tdgklcswsl emlsqpletl elhtkqskpi aatclafpyg dvnnfvvgse
      481 dgtvysacrh gtkagvlety eghqgpvtgi sahavqggid fshlfltssi dwtiklwslk
      541 enkplysfeh ngdyvydvaw spthpalfaa vddsgrldlw nlnqdtevpt asvlidgcpa
      601 lnrvswtpsg lhvtvgddtg kiwvydvaeh lahpridewn kflytqqelk hnkadeelhk
      661 lnlrepaslt sihpllttgp lr
//
LOCUS       XP_034186362             681 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X18
            [Osmia lignaria lignaria].
ACCESSION   XP_034186362
VERSION     XP_034186362.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330471.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..681
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X18"
                     /calculated_mol_wt=76463
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <309..627
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          310..355
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          412..451
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          461..500
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          507..548
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          554..594
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..681
                     /gene="sw"
                     /coded_by="XM_034330471.2:173..2218"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkggs apnylshafd yyvltfddgq
      181 aedeenslph mdgfqsklpp gilphglpqv kevqpavtqv eqekekekpk evrelseeek
      241 qmiilsedfq rfldrtsriv eralgesvdi ytdytgtmdg edgmdekshq rlwlnrsffc
      301 drwsrnrcvt smdwspqfpe llaasynnnd dipndpdgvc lvwntkfkkt tpefifhcqs
      361 pvmsttfarf hpnlilggty sgqivlwdnr vqkrspiqrt plsasahthp vyclnivgaq
      421 nahnlisist dgklcswsle mlsqpletle lhtkqskpia atclafpygd vnnfvvgsed
      481 gtvysacrhg tkagvletye ghqgpvtgis ahavqggidf shlfltssid wtiklwslke
      541 nkplysfehn gdyvydvaws pthpalfaav ddsgrldlwn lnqdtevpta svlidgcpal
      601 nrvswtpsgl hvtvgddtgk iwvydvaehl ahpridewnk flytqqelkh nkadeelhkl
      661 nlrepaslts ihpllttgpl r
//
LOCUS       XP_034186363             681 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X19
            [Osmia lignaria lignaria].
ACCESSION   XP_034186363
VERSION     XP_034186363.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330472.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..681
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X19"
                     /calculated_mol_wt=76764
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <309..627
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          310..355
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          412..451
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          461..500
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          507..548
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          554..594
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..681
                     /gene="sw"
                     /coded_by="XM_034330472.2:173..2218"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkaha fdyydeynln pglewedeft
      181 aedeenslph mdgfqsklpp gilphglpqv kevqpavtqv eqekekekpk evrelseeek
      241 qmiilsedfq rfldrtsriv eralgesvdi ytdytgtmdg edgmdekshq rlwlnrsffc
      301 drwsrnrcvt smdwspqfpe llaasynnnd dipndpdgvc lvwntkfkkt tpefifhcqs
      361 pvmsttfarf hpnlilggty sgqivlwdnr vqkrspiqrt plsasahthp vyclnivgaq
      421 nahnlisist dgklcswsle mlsqpletle lhtkqskpia atclafpygd vnnfvvgsed
      481 gtvysacrhg tkagvletye ghqgpvtgis ahavqggidf shlfltssid wtiklwslke
      541 nkplysfehn gdyvydvaws pthpalfaav ddsgrldlwn lnqdtevpta svlidgcpal
      601 nrvswtpsgl hvtvgddtgk iwvydvaehl ahpridewnk flytqqelkh nkadeelhkl
      661 nlrepaslts ihpllttgpl r
//
LOCUS       XP_034186364             680 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X20
            [Osmia lignaria lignaria].
ACCESSION   XP_034186364
VERSION     XP_034186364.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330473.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..680
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X20"
                     /calculated_mol_wt=76677
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <308..626
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          309..354
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          411..450
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          460..499
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          506..547
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          553..593
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..680
                     /gene="sw"
                     /coded_by="XM_034330473.2:174..2216"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdgyfead wwrprkahaf dyydeynlnp glewedefta
      181 edeenslphm dgfqsklppg ilphglpqvk evqpavtqve qekekekpke vrelseeekq
      241 miilsedfqr fldrtsrive ralgesvdiy tdytgtmdge dgmdekshqr lwlnrsffcd
      301 rwsrnrcvts mdwspqfpel laasynnndd ipndpdgvcl vwntkfkktt pefifhcqsp
      361 vmsttfarfh pnlilggtys gqivlwdnrv qkrspiqrtp lsasahthpv yclnivgaqn
      421 ahnlisistd gklcswslem lsqpletlel htkqskpiaa tclafpygdv nnfvvgsedg
      481 tvysacrhgt kagvletyeg hqgpvtgisa havqggidfs hlfltssidw tiklwslken
      541 kplysfehng dyvydvawsp thpalfaavd dsgrldlwnl nqdtevptas vlidgcpaln
      601 rvswtpsglh vtvgddtgki wvydvaehla hpridewnkf lytqqelkhn kadeelhkln
      661 lrepasltsi hpllttgplr
//
LOCUS       XP_034186365             674 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X21
            [Osmia lignaria lignaria].
ACCESSION   XP_034186365
VERSION     XP_034186365.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330474.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..674
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X21"
                     /calculated_mol_wt=75896
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <302..620
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          303..348
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          405..444
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          454..493
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          500..541
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          547..587
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..674
                     /gene="sw"
                     /coded_by="XM_034330474.2:173..2197"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkdey nlnpglewed eftaedeens
      181 lphmdgfqsk lppgilphgl pqvkevqpav tqveqekeke kpkevrelse eekqmiilse
      241 dfqrfldrts riveralges vdiytdytgt mdgedgmdek shqrlwlnrs ffcdrwsrnr
      301 cvtsmdwspq fpellaasyn nnddipndpd gvclvwntkf kkttpefifh cqspvmsttf
      361 arfhpnlilg gtysgqivlw dnrvqkrspi qrtplsasah thpvyclniv gaqnahnlis
      421 istdgklcsw slemlsqple tlelhtkqsk piaatclafp ygdvnnfvvg sedgtvysac
      481 rhgtkagvle tyeghqgpvt gisahavqgg idfshlflts sidwtiklws lkenkplysf
      541 ehngdyvydv awspthpalf aavddsgrld lwnlnqdtev ptasvlidgc palnrvswtp
      601 sglhvtvgdd tgkiwvydva ehlahpride wnkflytqqe lkhnkadeel hklnlrepas
      661 ltsihplltt gplr
//
LOCUS       XP_034186366             674 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X22
            [Osmia lignaria lignaria].
ACCESSION   XP_034186366
VERSION     XP_034186366.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330475.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..674
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X22"
                     /calculated_mol_wt=75627
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          302..615
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          303..348
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(316,330,336..337,349..350,369,374,380..381,399..400,
                     419,424,430..431,445,469,473,494..495,519,523,529..530,
                     542..543,561,566,572..573,585..586,607,609,615)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          405..444
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          454..493
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          500..541
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          547..587
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..674
                     /gene="sw"
                     /coded_by="XM_034330475.2:173..2197"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkggs apnylfltfd dgqaedeens
      181 lphmdgfqsk lppgilphgl pqvkevqpav tqveqekeke kpkevrelse eekqmiilse
      241 dfqrfldrts riveralges vdiytdytgt mdgedgmdek shqrlwlnrs ffcdrwsrnr
      301 cvtsmdwspq fpellaasyn nnddipndpd gvclvwntkf kkttpefifh cqspvmsttf
      361 arfhpnlilg gtysgqivlw dnrvqkrspi qrtplsasah thpvyclniv gaqnahnlis
      421 istdgklcsw slemlsqple tlelhtkqsk piaatclafp ygdvnnfvvg sedgtvysac
      481 rhgtkagvle tyeghqgpvt gisahavqgg idfshlflts sidwtiklws lkenkplysf
      541 ehngdyvydv awspthpalf aavddsgrld lwnlnqdtev ptasvlidgc palnrvswtp
      601 sglhvtvgdd tgkiwvydva ehlahpride wnkflytqqe lkhnkadeel hklnlrepas
      661 ltsihplltt gplr
//
LOCUS       XP_034186368             673 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X23
            [Osmia lignaria lignaria].
ACCESSION   XP_034186368
VERSION     XP_034186368.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330477.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..673
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X23"
                     /calculated_mol_wt=75687
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <301..619
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          302..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          404..443
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          453..492
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          499..540
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          546..586
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..673
                     /gene="sw"
                     /coded_by="XM_034330477.2:173..2194"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkaha fdyyvltfdd gqaedeensl
      181 phmdgfqskl ppgilphglp qvkevqpavt qveqekekek pkevrelsee ekqmiilsed
      241 fqrfldrtsr iveralgesv diytdytgtm dgedgmdeks hqrlwlnrsf fcdrwsrnrc
      301 vtsmdwspqf pellaasynn nddipndpdg vclvwntkfk kttpefifhc qspvmsttfa
      361 rfhpnlilgg tysgqivlwd nrvqkrspiq rtplsasaht hpvyclnivg aqnahnlisi
      421 stdgklcsws lemlsqplet lelhtkqskp iaatclafpy gdvnnfvvgs edgtvysacr
      481 hgtkagvlet yeghqgpvtg isahavqggi dfshlfltss idwtiklwsl kenkplysfe
      541 hngdyvydva wspthpalfa avddsgrldl wnlnqdtevp tasvlidgcp alnrvswtps
      601 glhvtvgddt gkiwvydvae hlahpridew nkflytqqel khnkadeelh klnlrepasl
      661 tsihpllttg plr
//
LOCUS       XP_034186369             673 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X24
            [Osmia lignaria lignaria].
ACCESSION   XP_034186369
VERSION     XP_034186369.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330478.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..673
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X24"
                     /calculated_mol_wt=75587
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <301..619
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          302..347
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          404..443
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          453..492
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          499..540
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          546..586
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..673
                     /gene="sw"
                     /coded_by="XM_034330478.2:173..2194"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkggs apnylshafd yyaedeensl
      181 phmdgfqskl ppgilphglp qvkevqpavt qveqekekek pkevrelsee ekqmiilsed
      241 fqrfldrtsr iveralgesv diytdytgtm dgedgmdeks hqrlwlnrsf fcdrwsrnrc
      301 vtsmdwspqf pellaasynn nddipndpdg vclvwntkfk kttpefifhc qspvmsttfa
      361 rfhpnlilgg tysgqivlwd nrvqkrspiq rtplsasaht hpvyclnivg aqnahnlisi
      421 stdgklcsws lemlsqplet lelhtkqskp iaatclafpy gdvnnfvvgs edgtvysacr
      481 hgtkagvlet yeghqgpvtg isahavqggi dfshlfltss idwtiklwsl kenkplysfe
      541 hngdyvydva wspthpalfa avddsgrldl wnlnqdtevp tasvlidgcp alnrvswtps
      601 glhvtvgddt gkiwvydvae hlahpridew nkflytqqel khnkadeelh klnlrepasl
      661 tsihpllttg plr
//
LOCUS       XP_034186371             669 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X25
            [Osmia lignaria lignaria].
ACCESSION   XP_034186371
VERSION     XP_034186371.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330480.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..669
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X25"
                     /calculated_mol_wt=75171
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <297..615
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          298..343
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          400..439
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          449..488
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          495..536
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          542..582
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..669
                     /gene="sw"
                     /coded_by="XM_034330480.2:173..2182"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdahafd yydeynlnpg lewedeftae deenslphmd
      181 gfqsklppgi lphglpqvke vqpavtqveq ekekekpkev relseeekqm iilsedfqrf
      241 ldrtsriver algesvdiyt dytgtmdged gmdekshqrl wlnrsffcdr wsrnrcvtsm
      301 dwspqfpell aasynnnddi pndpdgvclv wntkfkkttp efifhcqspv msttfarfhp
      361 nlilggtysg qivlwdnrvq krspiqrtpl sasahthpvy clnivgaqna hnlisistdg
      421 klcswsleml sqpletlelh tkqskpiaat clafpygdvn nfvvgsedgt vysacrhgtk
      481 agvletyegh qgpvtgisah avqggidfsh lfltssidwt iklwslkenk plysfehngd
      541 yvydvawspt hpalfaavdd sgrldlwnln qdtevptasv lidgcpalnr vswtpsglhv
      601 tvgddtgkiw vydvaehlah pridewnkfl ytqqelkhnk adeelhklnl repasltsih
      661 pllttgplr
//
LOCUS       XP_034186372             668 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X26
            [Osmia lignaria lignaria].
ACCESSION   XP_034186372
VERSION     XP_034186372.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330481.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X26"
                     /calculated_mol_wt=75084
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <296..614
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          297..342
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          399..438
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          448..487
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          494..535
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          541..581
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..668
                     /gene="sw"
                     /coded_by="XM_034330481.2:174..2180"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdahafdy ydeynlnpgl ewedeftaed eenslphmdg
      181 fqsklppgil phglpqvkev qpavtqveqe kekekpkevr elseeekqmi ilsedfqrfl
      241 drtsrivera lgesvdiytd ytgtmdgedg mdekshqrlw lnrsffcdrw srnrcvtsmd
      301 wspqfpella asynnnddip ndpdgvclvw ntkfkkttpe fifhcqspvm sttfarfhpn
      361 lilggtysgq ivlwdnrvqk rspiqrtpls asahthpvyc lnivgaqnah nlisistdgk
      421 lcswslemls qpletlelht kqskpiaatc lafpygdvnn fvvgsedgtv ysacrhgtka
      481 gvletyeghq gpvtgisaha vqggidfshl fltssidwti klwslkenkp lysfehngdy
      541 vydvawspth palfaavdds grldlwnlnq dtevptasvl idgcpalnrv swtpsglhvt
      601 vgddtgkiwv ydvaehlahp ridewnkfly tqqelkhnka deelhklnlr epasltsihp
      661 llttgplr
//
LOCUS       XP_034186373             666 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X27
            [Osmia lignaria lignaria].
ACCESSION   XP_034186373
VERSION     XP_034186373.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330482.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..666
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X27"
                     /calculated_mol_wt=74819
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          294..607
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          295..340
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Site            order(308,322,328..329,341..342,361,366,372..373,391..392,
                     411,416,422..423,437,461,465,486..487,511,515,521..522,
                     534..535,553,558,564..565,577..578,599,601,607)
                     /site_type="active"
                     /note="structural tetrad [active]"
                     /db_xref="CDD:238121"
     Region          397..436
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          446..485
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          492..533
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          539..579
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..666
                     /gene="sw"
                     /coded_by="XM_034330482.2:173..2173"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkvlt fddgqaedee nslphmdgfq
      181 sklppgilph glpqvkevqp avtqveqeke kekpkevrel seeekqmiil sedfqrfldr
      241 tsriveralg esvdiytdyt gtmdgedgmd ekshqrlwln rsffcdrwsr nrcvtsmdws
      301 pqfpellaas ynnnddipnd pdgvclvwnt kfkkttpefi fhcqspvmst tfarfhpnli
      361 lggtysgqiv lwdnrvqkrs piqrtplsas ahthpvycln ivgaqnahnl isistdgklc
      421 swslemlsqp letlelhtkq skpiaatcla fpygdvnnfv vgsedgtvys acrhgtkagv
      481 letyeghqgp vtgisahavq ggidfshlfl tssidwtikl wslkenkply sfehngdyvy
      541 dvawspthpa lfaavddsgr ldlwnlnqdt evptasvlid gcpalnrvsw tpsglhvtvg
      601 ddtgkiwvyd vaehlahpri dewnkflytq qelkhnkade elhklnlrep asltsihpll
      661 ttgplr
//
LOCUS       XP_034186374             666 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X28
            [Osmia lignaria lignaria].
ACCESSION   XP_034186374
VERSION     XP_034186374.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330483.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..666
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X28"
                     /calculated_mol_wt=74719
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <294..612
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          295..340
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          397..436
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          446..485
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          492..533
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          539..579
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..666
                     /gene="sw"
                     /coded_by="XM_034330483.2:173..2173"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkggs apnylsedee nslphmdgfq
      181 sklppgilph glpqvkevqp avtqveqeke kekpkevrel seeekqmiil sedfqrfldr
      241 tsriveralg esvdiytdyt gtmdgedgmd ekshqrlwln rsffcdrwsr nrcvtsmdws
      301 pqfpellaas ynnnddipnd pdgvclvwnt kfkkttpefi fhcqspvmst tfarfhpnli
      361 lggtysgqiv lwdnrvqkrs piqrtplsas ahthpvycln ivgaqnahnl isistdgklc
      421 swslemlsqp letlelhtkq skpiaatcla fpygdvnnfv vgsedgtvys acrhgtkagv
      481 letyeghqgp vtgisahavq ggidfshlfl tssidwtikl wslkenkply sfehngdyvy
      541 dvawspthpa lfaavddsgr ldlwnlnqdt evptasvlid gcpalnrvsw tpsglhvtvg
      601 ddtgkiwvyd vaehlahpri dewnkflytq qelkhnkade elhklnlrep asltsihpll
      661 ttgplr
//
LOCUS       XP_034186375             665 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X29
            [Osmia lignaria lignaria].
ACCESSION   XP_034186375
VERSION     XP_034186375.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330484.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..665
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X29"
                     /calculated_mol_wt=74811
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <293..611
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          294..339
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          396..435
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          445..484
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          491..532
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          538..578
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..665
                     /gene="sw"
                     /coded_by="XM_034330484.2:173..2170"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkaha fdyyaedeen slphmdgfqs
      181 klppgilphg lpqvkevqpa vtqveqekek ekpkevrels eeekqmiils edfqrfldrt
      241 sriveralge svdiytdytg tmdgedgmde kshqrlwlnr sffcdrwsrn rcvtsmdwsp
      301 qfpellaasy nnnddipndp dgvclvwntk fkkttpefif hcqspvmstt farfhpnlil
      361 ggtysgqivl wdnrvqkrsp iqrtplsasa hthpvyclni vgaqnahnli sistdgklcs
      421 wslemlsqpl etlelhtkqs kpiaatclaf pygdvnnfvv gsedgtvysa crhgtkagvl
      481 etyeghqgpv tgisahavqg gidfshlflt ssidwtiklw slkenkplys fehngdyvyd
      541 vawspthpal faavddsgrl dlwnlnqdte vptasvlidg cpalnrvswt psglhvtvgd
      601 dtgkiwvydv aehlahprid ewnkflytqq elkhnkadee lhklnlrepa sltsihpllt
      661 tgplr
//
LOCUS       XP_034186376             662 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X30
            [Osmia lignaria lignaria].
ACCESSION   XP_034186376
VERSION     XP_034186376.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..662
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X30"
                     /calculated_mol_wt=74303
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <290..608
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          291..336
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          393..432
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          442..481
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          488..529
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          535..575
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..662
                     /gene="sw"
                     /coded_by="XM_034330485.2:173..2161"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshddeynl npglewedef taedeenslp hmdgfqsklp
      181 pgilphglpq vkevqpavtq veqekekekp kevrelseee kqmiilsedf qrfldrtsri
      241 veralgesvd iytdytgtmd gedgmdeksh qrlwlnrsff cdrwsrnrcv tsmdwspqfp
      301 ellaasynnn ddipndpdgv clvwntkfkk ttpefifhcq spvmsttfar fhpnlilggt
      361 ysgqivlwdn rvqkrspiqr tplsasahth pvyclnivga qnahnlisis tdgklcswsl
      421 emlsqpletl elhtkqskpi aatclafpyg dvnnfvvgse dgtvysacrh gtkagvlety
      481 eghqgpvtgi sahavqggid fshlfltssi dwtiklwslk enkplysfeh ngdyvydvaw
      541 spthpalfaa vddsgrldlw nlnqdtevpt asvlidgcpa lnrvswtpsg lhvtvgddtg
      601 kiwvydvaeh lahpridewn kflytqqelk hnkadeelhk lnlrepaslt sihpllttgp
      661 lr
//
LOCUS       XP_034186377             661 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X31
            [Osmia lignaria lignaria].
ACCESSION   XP_034186377
VERSION     XP_034186377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X31"
                     /calculated_mol_wt=74094
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <289..607
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          290..335
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          392..431
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          441..480
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          487..528
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          534..574
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..661
                     /gene="sw"
                     /coded_by="XM_034330486.2:173..2158"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdahafd yyvltfddgq aedeenslph mdgfqsklpp
      181 gilphglpqv kevqpavtqv eqekekekpk evrelseeek qmiilsedfq rfldrtsriv
      241 eralgesvdi ytdytgtmdg edgmdekshq rlwlnrsffc drwsrnrcvt smdwspqfpe
      301 llaasynnnd dipndpdgvc lvwntkfkkt tpefifhcqs pvmsttfarf hpnlilggty
      361 sgqivlwdnr vqkrspiqrt plsasahthp vyclnivgaq nahnlisist dgklcswsle
      421 mlsqpletle lhtkqskpia atclafpygd vnnfvvgsed gtvysacrhg tkagvletye
      481 ghqgpvtgis ahavqggidf shlfltssid wtiklwslke nkplysfehn gdyvydvaws
      541 pthpalfaav ddsgrldlwn lnqdtevpta svlidgcpal nrvswtpsgl hvtvgddtgk
      601 iwvydvaehl ahpridewnk flytqqelkh nkadeelhkl nlrepaslts ihpllttgpl
      661 r
//
LOCUS       XP_034186378             661 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X32
            [Osmia lignaria lignaria].
ACCESSION   XP_034186378
VERSION     XP_034186378.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330487.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X32"
                     /calculated_mol_wt=74216
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <289..607
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          290..335
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          392..431
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          441..480
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          487..528
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          534..574
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..661
                     /gene="sw"
                     /coded_by="XM_034330487.2:174..2159"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshddeynln pglewedeft aedeenslph mdgfqsklpp
      181 gilphglpqv kevqpavtqv eqekekekpk evrelseeek qmiilsedfq rfldrtsriv
      241 eralgesvdi ytdytgtmdg edgmdekshq rlwlnrsffc drwsrnrcvt smdwspqfpe
      301 llaasynnnd dipndpdgvc lvwntkfkkt tpefifhcqs pvmsttfarf hpnlilggty
      361 sgqivlwdnr vqkrspiqrt plsasahthp vyclnivgaq nahnlisist dgklcswsle
      421 mlsqpletle lhtkqskpia atclafpygd vnnfvvgsed gtvysacrhg tkagvletye
      481 ghqgpvtgis ahavqggidf shlfltssid wtiklwslke nkplysfehn gdyvydvaws
      541 pthpalfaav ddsgrldlwn lnqdtevpta svlidgcpal nrvswtpsgl hvtvgddtgk
      601 iwvydvaehl ahpridewnk flytqqelkh nkadeelhkl nlrepaslts ihpllttgpl
      661 r
//
LOCUS       XP_034186379             660 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X33
            [Osmia lignaria lignaria].
ACCESSION   XP_034186379
VERSION     XP_034186379.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330488.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..660
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X33"
                     /calculated_mol_wt=74007
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <288..606
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          289..334
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          391..430
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          440..479
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          486..527
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          533..573
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..660
                     /gene="sw"
                     /coded_by="XM_034330488.2:174..2156"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdahafdy yvltfddgqa edeenslphm dgfqsklppg
      181 ilphglpqvk evqpavtqve qekekekpke vrelseeekq miilsedfqr fldrtsrive
      241 ralgesvdiy tdytgtmdge dgmdekshqr lwlnrsffcd rwsrnrcvts mdwspqfpel
      301 laasynnndd ipndpdgvcl vwntkfkktt pefifhcqsp vmsttfarfh pnlilggtys
      361 gqivlwdnrv qkrspiqrtp lsasahthpv yclnivgaqn ahnlisistd gklcswslem
      421 lsqpletlel htkqskpiaa tclafpygdv nnfvvgsedg tvysacrhgt kagvletyeg
      481 hqgpvtgisa havqggidfs hlfltssidw tiklwslken kplysfehng dyvydvawsp
      541 thpalfaavd dsgrldlwnl nqdtevptas vlidgcpaln rvswtpsglh vtvgddtgki
      601 wvydvaehla hpridewnkf lytqqelkhn kadeelhkln lrepasltsi hpllttgplr
//
LOCUS       XP_034186380             658 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X34
            [Osmia lignaria lignaria].
ACCESSION   XP_034186380
VERSION     XP_034186380.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..658
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X34"
                     /calculated_mol_wt=73943
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <286..604
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          287..332
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          389..428
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          438..477
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          484..525
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          531..571
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..658
                     /gene="sw"
                     /coded_by="XM_034330489.2:173..2149"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdgyfea dwwrprkaed eenslphmdg fqsklppgil
      181 phglpqvkev qpavtqveqe kekekpkevr elseeekqmi ilsedfqrfl drtsrivera
      241 lgesvdiytd ytgtmdgedg mdekshqrlw lnrsffcdrw srnrcvtsmd wspqfpella
      301 asynnnddip ndpdgvclvw ntkfkkttpe fifhcqspvm sttfarfhpn lilggtysgq
      361 ivlwdnrvqk rspiqrtpls asahthpvyc lnivgaqnah nlisistdgk lcswslemls
      421 qpletlelht kqskpiaatc lafpygdvnn fvvgsedgtv ysacrhgtka gvletyeghq
      481 gpvtgisaha vqggidfshl fltssidwti klwslkenkp lysfehngdy vydvawspth
      541 palfaavdds grldlwnlnq dtevptasvl idgcpalnrv swtpsglhvt vgddtgkiwv
      601 ydvaehlahp ridewnkfly tqqelkhnka deelhklnlr epasltsihp llttgplr
//
LOCUS       XP_034186381             654 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X35
            [Osmia lignaria lignaria].
ACCESSION   XP_034186381
VERSION     XP_034186381.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330490.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..654
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X35"
                     /calculated_mol_wt=73226
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <282..600
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          283..328
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          385..424
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          434..473
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          480..521
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          527..567
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..654
                     /gene="sw"
                     /coded_by="XM_034330490.2:173..2137"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdvltfd dgqaedeens lphmdgfqsk lppgilphgl
      181 pqvkevqpav tqveqekeke kpkevrelse eekqmiilse dfqrfldrts riveralges
      241 vdiytdytgt mdgedgmdek shqrlwlnrs ffcdrwsrnr cvtsmdwspq fpellaasyn
      301 nnddipndpd gvclvwntkf kkttpefifh cqspvmsttf arfhpnlilg gtysgqivlw
      361 dnrvqkrspi qrtplsasah thpvyclniv gaqnahnlis istdgklcsw slemlsqple
      421 tlelhtkqsk piaatclafp ygdvnnfvvg sedgtvysac rhgtkagvle tyeghqgpvt
      481 gisahavqgg idfshlflts sidwtiklws lkenkplysf ehngdyvydv awspthpalf
      541 aavddsgrld lwnlnqdtev ptasvlidgc palnrvswtp sglhvtvgdd tgkiwvydva
      601 ehlahpride wnkflytqqe lkhnkadeel hklnlrepas ltsihplltt gplr
//
LOCUS       XP_034186382             653 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X36
            [Osmia lignaria lignaria].
ACCESSION   XP_034186382
VERSION     XP_034186382.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330491.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..653
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X36"
                     /calculated_mol_wt=73218
     Region          107..132
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <281..599
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          282..327
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          384..423
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          433..472
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          479..520
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          526..566
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..653
                     /gene="sw"
                     /coded_by="XM_034330491.2:173..2134"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qssagrkknr eltivsvaht
      121 nippkepvvy skqtqtiqtt htshdahafd yyaedeensl phmdgfqskl ppgilphglp
      181 qvkevqpavt qveqekekek pkevrelsee ekqmiilsed fqrfldrtsr iveralgesv
      241 diytdytgtm dgedgmdeks hqrlwlnrsf fcdrwsrnrc vtsmdwspqf pellaasynn
      301 nddipndpdg vclvwntkfk kttpefifhc qspvmsttfa rfhpnlilgg tysgqivlwd
      361 nrvqkrspiq rtplsasaht hpvyclnivg aqnahnlisi stdgklcsws lemlsqplet
      421 lelhtkqskp iaatclafpy gdvnnfvvgs edgtvysacr hgtkagvlet yeghqgpvtg
      481 isahavqggi dfshlfltss idwtiklwsl kenkplysfe hngdyvydva wspthpalfa
      541 avddsgrldl wnlnqdtevp tasvlidgcp alnrvswtps glhvtvgddt gkiwvydvae
      601 hlahpridew nkflytqqel khnkadeelh klnlrepasl tsihpllttg plr
//
LOCUS       XP_034186384             653 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X37
            [Osmia lignaria lignaria].
ACCESSION   XP_034186384
VERSION     XP_034186384.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330493.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..653
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X37"
                     /calculated_mol_wt=73139
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <281..599
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          282..327
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          384..423
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          433..472
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          479..520
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          526..566
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..653
                     /gene="sw"
                     /coded_by="XM_034330493.2:174..2135"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdvltfdd gqaedeensl phmdgfqskl ppgilphglp
      181 qvkevqpavt qveqekekek pkevrelsee ekqmiilsed fqrfldrtsr iveralgesv
      241 diytdytgtm dgedgmdeks hqrlwlnrsf fcdrwsrnrc vtsmdwspqf pellaasynn
      301 nddipndpdg vclvwntkfk kttpefifhc qspvmsttfa rfhpnlilgg tysgqivlwd
      361 nrvqkrspiq rtplsasaht hpvyclnivg aqnahnlisi stdgklcsws lemlsqplet
      421 lelhtkqskp iaatclafpy gdvnnfvvgs edgtvysacr hgtkagvlet yeghqgpvtg
      481 isahavqggi dfshlfltss idwtiklwsl kenkplysfe hngdyvydva wspthpalfa
      541 avddsgrldl wnlnqdtevp tasvlidgcp alnrvswtps glhvtvgddt gkiwvydvae
      601 hlahpridew nkflytqqel khnkadeelh klnlrepasl tsihpllttg plr
//
LOCUS       XP_034186385             652 aa            linear   INV 03-SEP-2025
DEFINITION  cytoplasmic dynein 1 intermediate chain short wing isoform X38
            [Osmia lignaria lignaria].
ACCESSION   XP_034186385
VERSION     XP_034186385.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330494.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..652
                     /product="cytoplasmic dynein 1 intermediate chain short
                     wing isoform X38"
                     /calculated_mol_wt=73131
     Region          106..131
                     /region_name="Dynein_IC2"
                     /note="Cytoplasmic dynein 1 intermediate chain 2;
                     pfam11540"
                     /db_xref="CDD:463291"
     Region          <280..598
                     /region_name="WD40"
                     /note="WD40 repeat [General function prediction only];
                     COG2319"
                     /db_xref="CDD:441893"
     Region          281..326
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          383..422
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          432..471
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          478..519
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          525..565
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..652
                     /gene="sw"
                     /coded_by="XM_034330494.2:174..2132"
                     /db_xref="GeneID:117607159"
ORIGIN      
        1 mmsdrkaele rkkaklqair eekerrrrek eqkdieeatv raagtdkdhr keldamlssl
       61 gvapvsdvls slssmnsltp eqsanatpda slqpssinsa qsagrkknre ltivsvahtn
      121 ippkepvvys kqtqtiqtth tshdahafdy yaedeenslp hmdgfqsklp pgilphglpq
      181 vkevqpavtq veqekekekp kevrelseee kqmiilsedf qrfldrtsri veralgesvd
      241 iytdytgtmd gedgmdeksh qrlwlnrsff cdrwsrnrcv tsmdwspqfp ellaasynnn
      301 ddipndpdgv clvwntkfkk ttpefifhcq spvmsttfar fhpnlilggt ysgqivlwdn
      361 rvqkrspiqr tplsasahth pvyclnivga qnahnlisis tdgklcswsl emlsqpletl
      421 elhtkqskpi aatclafpyg dvnnfvvgse dgtvysacrh gtkagvlety eghqgpvtgi
      481 sahavqggid fshlfltssi dwtiklwslk enkplysfeh ngdyvydvaw spthpalfaa
      541 vddsgrldlw nlnqdtevpt asvlidgcpa lnrvswtpsg lhvtvgddtg kiwvydvaeh
      601 lahpridewn kflytqqelk hnkadeelhk lnlrepaslt sihpllttgp lr
//
LOCUS       XP_034186386             575 aa            linear   INV 03-SEP-2025
DEFINITION  scavenger receptor class B member debris buster [Osmia lignaria
            lignaria].
ACCESSION   XP_034186386
VERSION     XP_034186386.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330495.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="scavenger receptor class B member debris buster"
                     /calculated_mol_wt=65000
     Region          68..520
                     /region_name="CD36"
                     /note="CD36 family; pfam01130"
                     /db_xref="CDD:460076"
     CDS             1..575
                     /gene="dsb"
                     /coded_by="XM_034330495.2:1391..3118"
                     /db_xref="GeneID:117607163"
ORIGIN      
        1 mkfigslfgi rspkkmgkey mkvgrnalfg vktsqhdglc tdrvprplsf liskngklkh
       61 grlsaifigi ltlvigiils sipwvdyvil kqlrlwngsl sfqywqkpgv vrltkvyifn
      121 vtnpenflqy nekpklqevg pfiyredmek vnivfhnngt vsyqhkkiln fvpemskden
      181 lkvivpnipl ltlstqsksl prfltvglsv flsglhmksf vpvtaqelvf gyddplvsia
      241 hrffpktrrp msrmglllgr ngtlnevsti ytghtdmkef glinrlngld hlpywpnapc
      301 nsirasegsf fpprektgad vvhiwdkdic rtlplkyrgp teksgikadl ytpsdvvfgr
      361 pnettpenec fcsddmstcp snglqnispc qysapvylsf phfykadpkl ldavhglkpd
      421 hdthgtyfki qpklgvpleg kvrvqlnlkv ehqphigvvs nfpdivfpim wieegieelt
      481 psirrwvyla ttfsdvavpc tsyglilvgm iiittvflka ynnavfthea ielgkrtirr
      541 sstllvngqh kllmtresyv llnsdnmekp nriev
//
LOCUS       XP_076548062             665 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607167 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548062
VERSION     XP_076548062.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691947.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..665
                     /product="uncharacterized protein LOC117607167 isoform X1"
                     /calculated_mol_wt=75976
     CDS             1..665
                     /gene="LOC117607167"
                     /coded_by="XM_076691947.1:2176..4173"
                     /db_xref="GeneID:117607167"
ORIGIN      
        1 mkttnwicll vacvclvear ykirrpplpp lqpaklmykk awpmthrist gnmmhingnv
       61 pkrmvpklpn yrirrppmfs mppviwkqqm pmraplnnha vlpqrppvke fhsvnkmpey
      121 speyrpeaav lppthrgsvd ddkgpihtip apnlspmdkp fngemnaeem aqrqqttdaa
      181 ykinahvgsi lqnfdlatig tslapqkpvt sptptqhqye vtetngvsqk eyqtvlptfi
      241 ppeyatlstl inpdfqsndv flnnhpasni gligtniqlg qpnlpmqtnl hsslhvgfpg
      301 tagqtpyiin qqipdlhvgh papagpplsa tqlydllnsf pqkipeqyqa gqqpqlqqhi
      361 lqqqlgqffq ptgvtgfsqp qmqsfnydeq dnkeqqqqqq qqqqqqqilf npeyvaervn
      421 tnynveqevs iddqennnlr qddelayine gaeqlennid yedtntqkgq ttyfnkvsnn
      481 ngissqfytt lpnreaaekl aalaaagnvn sqligqlrkq qqqhenmqne eamppnhkne
      541 dydqtyqdrt hnrqkhrqsq qeqeqqlqky eeqqkeyerq qeqhrqrqsa vysnkrplri
      601 mvpdendynn esqnnqsken teveyeyend eadalnsqsn ssydgrnyeq snaefgsrls
      661 pknga
//
LOCUS       XP_034186392             665 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607167 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186392
VERSION     XP_034186392.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330501.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..665
                     /product="uncharacterized protein LOC117607167 isoform X1"
                     /calculated_mol_wt=75976
     CDS             1..665
                     /gene="LOC117607167"
                     /coded_by="XM_034330501.2:1965..3962"
                     /db_xref="GeneID:117607167"
ORIGIN      
        1 mkttnwicll vacvclvear ykirrpplpp lqpaklmykk awpmthrist gnmmhingnv
       61 pkrmvpklpn yrirrppmfs mppviwkqqm pmraplnnha vlpqrppvke fhsvnkmpey
      121 speyrpeaav lppthrgsvd ddkgpihtip apnlspmdkp fngemnaeem aqrqqttdaa
      181 ykinahvgsi lqnfdlatig tslapqkpvt sptptqhqye vtetngvsqk eyqtvlptfi
      241 ppeyatlstl inpdfqsndv flnnhpasni gligtniqlg qpnlpmqtnl hsslhvgfpg
      301 tagqtpyiin qqipdlhvgh papagpplsa tqlydllnsf pqkipeqyqa gqqpqlqqhi
      361 lqqqlgqffq ptgvtgfsqp qmqsfnydeq dnkeqqqqqq qqqqqqqilf npeyvaervn
      421 tnynveqevs iddqennnlr qddelayine gaeqlennid yedtntqkgq ttyfnkvsnn
      481 ngissqfytt lpnreaaekl aalaaagnvn sqligqlrkq qqqhenmqne eamppnhkne
      541 dydqtyqdrt hnrqkhrqsq qeqeqqlqky eeqqkeyerq qeqhrqrqsa vysnkrplri
      601 mvpdendynn esqnnqsken teveyeyend eadalnsqsn ssydgrnyeq snaefgsrls
      661 pknga
//
LOCUS       XP_076548064             665 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607167 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548064
VERSION     XP_076548064.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691949.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..665
                     /product="uncharacterized protein LOC117607167 isoform X1"
                     /calculated_mol_wt=75976
     CDS             1..665
                     /gene="LOC117607167"
                     /coded_by="XM_076691949.1:2079..4076"
                     /db_xref="GeneID:117607167"
ORIGIN      
        1 mkttnwicll vacvclvear ykirrpplpp lqpaklmykk awpmthrist gnmmhingnv
       61 pkrmvpklpn yrirrppmfs mppviwkqqm pmraplnnha vlpqrppvke fhsvnkmpey
      121 speyrpeaav lppthrgsvd ddkgpihtip apnlspmdkp fngemnaeem aqrqqttdaa
      181 ykinahvgsi lqnfdlatig tslapqkpvt sptptqhqye vtetngvsqk eyqtvlptfi
      241 ppeyatlstl inpdfqsndv flnnhpasni gligtniqlg qpnlpmqtnl hsslhvgfpg
      301 tagqtpyiin qqipdlhvgh papagpplsa tqlydllnsf pqkipeqyqa gqqpqlqqhi
      361 lqqqlgqffq ptgvtgfsqp qmqsfnydeq dnkeqqqqqq qqqqqqqilf npeyvaervn
      421 tnynveqevs iddqennnlr qddelayine gaeqlennid yedtntqkgq ttyfnkvsnn
      481 ngissqfytt lpnreaaekl aalaaagnvn sqligqlrkq qqqhenmqne eamppnhkne
      541 dydqtyqdrt hnrqkhrqsq qeqeqqlqky eeqqkeyerq qeqhrqrqsa vysnkrplri
      601 mvpdendynn esqnnqsken teveyeyend eadalnsqsn ssydgrnyeq snaefgsrls
      661 pknga
//
LOCUS       XP_034186393             665 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607167 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186393
VERSION     XP_034186393.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330502.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..665
                     /product="uncharacterized protein LOC117607167 isoform X1"
                     /calculated_mol_wt=75976
     CDS             1..665
                     /gene="LOC117607167"
                     /coded_by="XM_034330502.2:2093..4090"
                     /db_xref="GeneID:117607167"
ORIGIN      
        1 mkttnwicll vacvclvear ykirrpplpp lqpaklmykk awpmthrist gnmmhingnv
       61 pkrmvpklpn yrirrppmfs mppviwkqqm pmraplnnha vlpqrppvke fhsvnkmpey
      121 speyrpeaav lppthrgsvd ddkgpihtip apnlspmdkp fngemnaeem aqrqqttdaa
      181 ykinahvgsi lqnfdlatig tslapqkpvt sptptqhqye vtetngvsqk eyqtvlptfi
      241 ppeyatlstl inpdfqsndv flnnhpasni gligtniqlg qpnlpmqtnl hsslhvgfpg
      301 tagqtpyiin qqipdlhvgh papagpplsa tqlydllnsf pqkipeqyqa gqqpqlqqhi
      361 lqqqlgqffq ptgvtgfsqp qmqsfnydeq dnkeqqqqqq qqqqqqqilf npeyvaervn
      421 tnynveqevs iddqennnlr qddelayine gaeqlennid yedtntqkgq ttyfnkvsnn
      481 ngissqfytt lpnreaaekl aalaaagnvn sqligqlrkq qqqhenmqne eamppnhkne
      541 dydqtyqdrt hnrqkhrqsq qeqeqqlqky eeqqkeyerq qeqhrqrqsa vysnkrplri
      601 mvpdendynn esqnnqsken teveyeyend eadalnsqsn ssydgrnyeq snaefgsrls
      661 pknga
//
LOCUS       XP_034186394             664 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607167 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186394
VERSION     XP_034186394.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330503.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..664
                     /product="uncharacterized protein LOC117607167 isoform X2"
                     /calculated_mol_wt=75877
     CDS             1..664
                     /gene="LOC117607167"
                     /coded_by="XM_034330503.2:2176..4170"
                     /db_xref="GeneID:117607167"
ORIGIN      
        1 mkttnwicll vacvclvear ykirrpplpp lqpaklmykk awpmthrist gnmmhingnv
       61 pkrmvpklpn yrirrppmfs mppviwkqqm pmraplnnha vlpqrppvke fhsvnkmpey
      121 speyrpeaav lppthrgsvd ddkgpihtip apnlspmdkp fngemnaeem aqrqqttdaa
      181 ykinahgsil qnfdlatigt slapqkpvts ptptqhqyev tetngvsqke yqtvlptfip
      241 peyatlstli npdfqsndvf lnnhpasnig ligtniqlgq pnlpmqtnlh sslhvgfpgt
      301 agqtpyiinq qipdlhvghp apagpplsat qlydllnsfp qkipeqyqag qqpqlqqhil
      361 qqqlgqffqp tgvtgfsqpq mqsfnydeqd nkeqqqqqqq qqqqqqilfn peyvaervnt
      421 nynveqevsi ddqennnlrq ddelayineg aeqlennidy edtntqkgqt tyfnkvsnnn
      481 gissqfyttl pnreaaekla alaaagnvns qligqlrkqq qqhenmqnee amppnhkned
      541 ydqtyqdrth nrqkhrqsqq eqeqqlqkye eqqkeyerqq eqhrqrqsav ysnkrplrim
      601 vpdendynne sqnnqskent eveyeyende adalnsqsns sydgrnyeqs naefgsrlsp
      661 knga
//
LOCUS       XP_076548065             629 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607167 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548065
VERSION     XP_076548065.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691950.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="uncharacterized protein LOC117607167 isoform X3"
                     /calculated_mol_wt=71858
     CDS             1..629
                     /gene="LOC117607167"
                     /coded_by="XM_076691950.1:3161..5050"
                     /db_xref="GeneID:117607167"
ORIGIN      
        1 mykkawpmth ristgnmmhi ngnvpkrmvp klpnyrirrp pmfsmppviw kqqmpmrapl
       61 nnhavlpqrp pvkefhsvnk mpeyspeyrp eaavlppthr gsvdddkgpi htipapnlsp
      121 mdkpfngemn aeemaqrqqt tdaaykinah vgsilqnfdl atigtslapq kpvtsptptq
      181 hqyevtetng vsqkeyqtvl ptfippeyat lstlinpdfq sndvflnnhp asnigligtn
      241 iqlgqpnlpm qtnlhsslhv gfpgtagqtp yiinqqipdl hvghpapagp plsatqlydl
      301 lnsfpqkipe qyqagqqpql qqhilqqqlg qffqptgvtg fsqpqmqsfn ydeqdnkeqq
      361 qqqqqqqqqq qilfnpeyva ervntnynve qevsiddqen nnlrqddela yinegaeqle
      421 nnidyedtnt qkgqttyfnk vsnnngissq fyttlpnrea aeklaalaaa gnvnsqligq
      481 lrkqqqqhen mqneeamppn hknedydqty qdrthnrqkh rqsqqeqeqq lqkyeeqqke
      541 yerqqeqhrq rqsavysnkr plrimvpden dynnesqnnq skenteveye yendeadaln
      601 sqsnssydgr nyeqsnaefg srlspknga
//
LOCUS       XP_076548066             629 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607167 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548066
VERSION     XP_076548066.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691951.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="uncharacterized protein LOC117607167 isoform X3"
                     /calculated_mol_wt=71858
     CDS             1..629
                     /gene="LOC117607167"
                     /coded_by="XM_076691951.1:2397..4286"
                     /db_xref="GeneID:117607167"
ORIGIN      
        1 mykkawpmth ristgnmmhi ngnvpkrmvp klpnyrirrp pmfsmppviw kqqmpmrapl
       61 nnhavlpqrp pvkefhsvnk mpeyspeyrp eaavlppthr gsvdddkgpi htipapnlsp
      121 mdkpfngemn aeemaqrqqt tdaaykinah vgsilqnfdl atigtslapq kpvtsptptq
      181 hqyevtetng vsqkeyqtvl ptfippeyat lstlinpdfq sndvflnnhp asnigligtn
      241 iqlgqpnlpm qtnlhsslhv gfpgtagqtp yiinqqipdl hvghpapagp plsatqlydl
      301 lnsfpqkipe qyqagqqpql qqhilqqqlg qffqptgvtg fsqpqmqsfn ydeqdnkeqq
      361 qqqqqqqqqq qilfnpeyva ervntnynve qevsiddqen nnlrqddela yinegaeqle
      421 nnidyedtnt qkgqttyfnk vsnnngissq fyttlpnrea aeklaalaaa gnvnsqligq
      481 lrkqqqqhen mqneeamppn hknedydqty qdrthnrqkh rqsqqeqeqq lqkyeeqqke
      541 yerqqeqhrq rqsavysnkr plrimvpden dynnesqnnq skenteveye yendeadaln
      601 sqsnssydgr nyeqsnaefg srlspknga
//
LOCUS       XP_034186847             457 aa            linear   INV 03-SEP-2025
DEFINITION  RYamide receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186847
VERSION     XP_034186847.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330956.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="RYamide receptor isoform X1"
                     /calculated_mol_wt=52726
     Region          60..347
                     /region_name="7tmA_leucokinin-like"
                     /note="leucokinin-like peptide receptor from tick and
                     related proteins, member of the class A family of
                     seven-transmembrane G protein-coupled receptors; cd15393"
                     /db_xref="CDD:320515"
     Region          61..87
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320515"
     Region          94..119
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320515"
     Site            order(116,119..120,132..137,139..140,143,186,188..192,223,
                     226..228,230..232,234..235,292,295..296,298..299,302,
                     314..315,317..319,322,325..326)
                     /site_type="other"
                     /note="putative peptide ligand binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:320515"
     Region          132..162
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320515"
     Region          173..194
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320515"
     Region          223..252
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320515"
     Region          272..302
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320515"
     Region          315..340
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320515"
     CDS             1..457
                     /gene="LOC117607353"
                     /coded_by="XM_034330956.2:838..2211"
                     /db_xref="GeneID:117607353"
ORIGIN      
        1 medplyasit idmndswlde sddfessllf sfnntyrirr nnsmyddfag syevptmilv
       61 vlsilygsis flavvgnslv mwivatsrrm qnvtnffian laladivigl ftipfqfqaa
      121 llqrwnlpyf mcafcpfvqv lsvnvsvftl taiaidrhra ilkplsakps kltakiiiag
      181 iwflagslat pmaiarrvvm epedytdhyk pfckevnlsd rsmliysgll vflqyltpls
      241 iiscvyarmg lklwgnkapg naedsrdanl lrnkmrvikm liivvalfai cwlplqtynv
      301 fryfhpkinn yeyihyiwff cdwlamsnsc ynpfiygiyn ekfrrefqqr cpfksrkwln
      361 nppmdniemd ktqstrtsvr yewkrtisgs yplvssfykg vtgrestqsf idehqrnarn
      421 kkgigystas nrseelyvfs sgkhkhaqdp dieelcl
//
LOCUS       XP_034186848             439 aa            linear   INV 03-SEP-2025
DEFINITION  RYamide receptor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186848
VERSION     XP_034186848.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330957.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186848.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..439
                     /product="RYamide receptor isoform X2"
                     /calculated_mol_wt=50450
     Region          42..329
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          44..68
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          77..99
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          115..137
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          158..174
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          206..229
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          260..282
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          297..322
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..439
                     /gene="LOC117607353"
                     /coded_by="XM_034330957.2:878..2197"
                     /db_xref="GeneID:117607353"
ORIGIN      
        1 mihgwtkvmi skvpyyfrst tptgfaeiiq sgsyevptmi lvvlsilygs isflavvgns
       61 lvmwivatsr rmqnvtnffi anlaladivi glftipfqfq aallqrwnlp yfmcafcpfv
      121 qvlsvnvsvf tltaiaidrh railkplsak pskltakiii agiwflagsl atpmaiarrv
      181 vmepedytdh ykpfckevnl sdrsmliysg llvflqyltp lsiiscvyar mglklwgnka
      241 pgnaedsrda nllrnkmrvi kmliivvalf aicwlplqty nvfryfhpki nnyeyihyiw
      301 ffcdwlamsn scynpfiygi ynekfrrefq qrcpfksrkw lnnppmdnie mdktqstrts
      361 vryewkrtis gsyplvssfy kgvtgrestq sfidehqrna rnkkgigyst asnrseelyv
      421 fssgkhkhaq dpdieelcl
//
LOCUS       XP_034186396             377 aa            linear   INV 03-SEP-2025
DEFINITION  methionine aminopeptidase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034186396
VERSION     XP_034186396.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..377
                     /product="methionine aminopeptidase 1"
                     /calculated_mol_wt=42266
     Region          3..372
                     /region_name="APP_MetAP"
                     /note="A family including aminopeptidase P, aminopeptidase
                     M, and prolidase. Also known as metallopeptidase family
                     M24. This family of enzymes is able to cleave amido-,
                     imido- and amidino-containing bonds. Members exibit
                     relatively narrow substrate specificity...; cl00279"
                     /db_xref="CDD:469704"
     Site            order(193,210,221,284,317,348)
                     /site_type="active"
                     /db_xref="CDD:238519"
     CDS             1..377
                     /gene="LOC117607168"
                     /coded_by="XM_034330505.2:113..1246"
                     /db_xref="GeneID:117607168"
ORIGIN      
        1 maltfgmcet pgcktmaslq cptclkigiq gsyfctqdcf krswkthkvi hqlakgtggd
       61 ktsneynpwp fyhytgklrp ykreprrevp eeikrpdyat hstgvsiseq avrgsaqikv
      121 lddeeiegmr vacklgrevl deaartcdvg vttaeidkav heacverdcy psplnyyqfp
      181 ascctsvnev ichgipdtrp lqngdicnvd vtvyhngfhg dlnetflvgn vkpevrklve
      241 vtyeclskai divrpgekyr eignviqkha qahgfsvvrs ycghgihrlf htapsvphya
      301 knkavgvmkp ghcftiepmi sqgtwrdemw pdtwtavtad gqwsaqfeht llvnetgcdi
      361 ltkrltndgk pwfmdrt
//
LOCUS       XP_034186397             226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607169 [Osmia lignaria lignaria].
ACCESSION   XP_034186397
VERSION     XP_034186397.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330506.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186397.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..226
                     /product="uncharacterized protein LOC117607169"
                     /calculated_mol_wt=24293
     CDS             1..226
                     /gene="LOC117607169"
                     /coded_by="XM_034330506.2:2323..3003"
                     /db_xref="GeneID:117607169"
ORIGIN      
        1 mkvlplvvlc ccsilvlses resskedlls rasaqlerla pyrrtsteqk asneqwqlld
       61 naskdanril reqrtafeiy kllpekmqid iakrigadra tiellgdpky iemmqdrikr
      121 sgskrdysgp eeydhppdgy hyggydtdhg ehhessgsds ggilkgsgsl iggiangiig
      181 glvsasssas kgsssvsass assssssssd sghkkpeygh tysvtm
//
LOCUS       XP_034186398             213 aa            linear   INV 03-SEP-2025
DEFINITION  spaetzle domain-containing protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186398
VERSION     XP_034186398.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330507.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..213
                     /product="spaetzle domain-containing protein isoform X1"
                     /calculated_mol_wt=24732
     Region          105..196
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..213
                     /gene="spz"
                     /coded_by="XM_034330507.2:317..958"
                     /db_xref="GeneID:117607170"
ORIGIN      
        1 mkfslstrvm kqktdskill ngiflmlife trgkpfesss epkvsisqvk aapickntty
       61 ceitphypte vvfnalqenp hlrnyenide mdvheqdedi peeeplcvst eqvvfpksae
      121 tkssewqyiv nqedfrqsvr iekcreegqq crviddfaeg yytkckqkyi yrqllavsan
      181 gsiyhesfrf pasccchief radiflksfk hkv
//
LOCUS       XP_034186399             213 aa            linear   INV 03-SEP-2025
DEFINITION  spaetzle domain-containing protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186399
VERSION     XP_034186399.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330508.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..213
                     /product="spaetzle domain-containing protein isoform X1"
                     /calculated_mol_wt=24732
     Region          105..196
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..213
                     /gene="spz"
                     /coded_by="XM_034330508.2:250..891"
                     /db_xref="GeneID:117607170"
ORIGIN      
        1 mkfslstrvm kqktdskill ngiflmlife trgkpfesss epkvsisqvk aapickntty
       61 ceitphypte vvfnalqenp hlrnyenide mdvheqdedi peeeplcvst eqvvfpksae
      121 tkssewqyiv nqedfrqsvr iekcreegqq crviddfaeg yytkckqkyi yrqllavsan
      181 gsiyhesfrf pasccchief radiflksfk hkv
//
LOCUS       XP_034186400             199 aa            linear   INV 03-SEP-2025
DEFINITION  spaetzle domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186400
VERSION     XP_034186400.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330509.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="spaetzle domain-containing protein isoform X2"
                     /calculated_mol_wt=23158
     Region          91..182
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..199
                     /gene="spz"
                     /coded_by="XM_034330509.2:517..1116"
                     /db_xref="GeneID:117607170"
ORIGIN      
        1 msavvfwfil lmlifetrgk pfesssepkv sisqvkaapi cknttyceit phyptevvfn
       61 alqenphlrn yenidemdvh eqdedipeee plcvsteqvv fpksaetkss ewqyivnqed
      121 frqsvriekc reegqqcrvi ddfaegyytk ckqkyiyrql lavsangsiy hesfrfpasc
      181 cchiefradi flksfkhkv
//
LOCUS       XP_034186401             199 aa            linear   INV 03-SEP-2025
DEFINITION  spaetzle domain-containing protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186401
VERSION     XP_034186401.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330510.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..199
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..199
                     /product="spaetzle domain-containing protein isoform X2"
                     /calculated_mol_wt=23158
     Region          91..182
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..199
                     /gene="spz"
                     /coded_by="XM_034330510.2:432..1031"
                     /db_xref="GeneID:117607170"
ORIGIN      
        1 msavvfwfil lmlifetrgk pfesssepkv sisqvkaapi cknttyceit phyptevvfn
       61 alqenphlrn yenidemdvh eqdedipeee plcvsteqvv fpksaetkss ewqyivnqed
      121 frqsvriekc reegqqcrvi ddfaegyytk ckqkyiyrql lavsangsiy hesfrfpasc
      181 cchiefradi flksfkhkv
//
LOCUS       XP_034186402             160 aa            linear   INV 03-SEP-2025
DEFINITION  PEST proteolytic signal-containing nuclear protein [Osmia lignaria
            lignaria].
ACCESSION   XP_034186402
VERSION     XP_034186402.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330511.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..160
                     /product="PEST proteolytic signal-containing nuclear
                     protein"
                     /calculated_mol_wt=17816
     Region          6..156
                     /region_name="PCNP"
                     /note="PEST, proteolytic signal-containing nuclear protein
                     family; pfam15473"
                     /db_xref="CDD:464736"
     CDS             1..160
                     /gene="LOC117607172"
                     /coded_by="XM_034330511.2:120..602"
                     /db_xref="GeneID:117607172"
ORIGIN      
        1 mskrnpgdey rkgssvyrae dsrgddrgrd rsplrsddrc eatnysqtda kkqkltfafg
       61 kkggmeqqkk giqiklgsts kptvkatpvq rptvasvfnt deeeepeemp aearmrmrni
      121 gretptsagp nsfgktkqgf cdskkifekt lkkameeank
//
LOCUS       XP_034186403             114 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S26 [Osmia lignaria lignaria].
ACCESSION   XP_034186403
VERSION     XP_034186403.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330512.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..114
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..114
                     /product="ribosomal protein S26"
                     /calculated_mol_wt=12986
     Region          1..104
                     /region_name="Ribosomal_S26e"
                     /note="Ribosomal protein S26e; pfam01283"
                     /db_xref="CDD:426179"
     CDS             1..114
                     /gene="RpS26"
                     /coded_by="XM_034330512.2:143..487"
                     /db_xref="GeneID:117607173"
ORIGIN      
        1 mtckrrnggr akhgrghvnp vrctncarcv pkdkaikkfv irniveaaav rditeasfys
       61 ayqlpklyak lhycvscaih skvvrnrska nrrirtppvr nfprdlrqgq qnrk
//
LOCUS       XP_034186342             109 aa            linear   INV 03-SEP-2025
DEFINITION  sterile alpha motif domain-containing protein aveugle [Osmia
            lignaria lignaria].
ACCESSION   XP_034186342
VERSION     XP_034186342.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330451.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..109
                     /product="sterile alpha motif domain-containing protein
                     aveugle"
                     /calculated_mol_wt=13398
     Region          20..94
                     /region_name="SAM_aveugle-like"
                     /note="SAM domain of aveugle-like subfamily; cd09510"
                     /db_xref="CDD:188909"
     Site            order(50..54,57..58,61,66,69..70)
                     /site_type="other"
                     /note="heterodimer interface ML [polypeptide binding]"
                     /db_xref="CDD:188909"
     CDS             1..109
                     /gene="ave"
                     /coded_by="XM_034330451.2:242..571"
                     /db_xref="GeneID:117607155"
ORIGIN      
        1 mveevvnstn kpknkttrpr pvylwnvldv qkwlrrhcsd yyqlyhekfl yhditgrvll
       61 rinenillrl gidneqhrmd iwreimklrl ktdmleikdi errnnmnfd
//
LOCUS       XP_034183354            1785 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605763 [Osmia lignaria lignaria].
ACCESSION   XP_034183354
VERSION     XP_034183354.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327463.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183354.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1785
                     /product="uncharacterized protein LOC117605763"
                     /calculated_mol_wt=197568
     Region          1492..1762
                     /region_name="PTKc"
                     /note="Catalytic domain of Protein Tyrosine Kinases;
                     cd00192"
                     /db_xref="CDD:270623"
     Site            order(1493..1497,1501,1520,1522,1569..1572,1575..1576,
                     1628,1632..1633,1635,1646,1664..1666,1669..1670,1679,1713)
                     /site_type="active"
                     /db_xref="CDD:270623"
     Site            order(1493..1494,1496..1497,1501,1520,1522,1569..1572,
                     1575..1576,1632..1633,1635,1646)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270623"
     Site            order(1628,1632,1664..1666,1669..1670,1679,1713)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270623"
     Site            order(1645..1652,1664..1666,1669..1672)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270623"
     CDS             1..1785
                     /gene="LOC117605763"
                     /coded_by="XM_034327463.2:679..6036"
                     /db_xref="GeneID:117605763"
ORIGIN      
        1 mtvnrsvvtm vlmlitsvii lvhvkdvmtl eyddllpane tnarhridte ssigdhrqgr
       61 mslqaelpss kakreqalrs ilaarrrqil sqrplesrnq nrtpkpaeas akepsdvlea
      121 qetkhgmyvt astngkiehs ideeltskdv diefiskeve skraidqnsk kssndnltas
      181 stddksyssd skidhstsqk nlalsssptt ssslvtnsqk aseteirkkn ivsmpeslyn
      241 yfrpvesnip vedmsqflyf gqkiqpetqn ntgnnsveat ptaptapssr rrysmkrytt
      301 ttpvsipmpr leeiineemn iaverqtvek nkvskiknaf rsaengvyyr ksrptestia
      361 snvitakeia gnstevgtkp sggkfvddnp sahadtpgql rhergsaath vvaetrnatn
      421 stqipyqqpv sanayeptet eseialktdr ptdtssesna mlnemehgsv tksstltrki
      481 ddsevveten vsviedknps esietemygd htekkikrid lyetnvefdr ksdgmhadqp
      541 rgsnsrennn ietstsreki grpstepfpi gwtspetstl riivtegslr qsfaigadaa
      601 ekytvkpvds teervrsvas gdlpttkapl pstttttsgs ssvatieaal arpksrhreq
      661 kqqrqrpegn kvdreenqdr qqhrhqeqqq qhvyaytitn vtrllrnytg pglsreeegg
      721 aerstgttei tsgsvqprqp pppsllllpr agsgieaara pvelsagttv eavaagrasv
      781 ahlvgerqrk vvavvpqsqq ravnrtsvql tsvnkngpws irttessnsg srgavrwnht
      841 ktrntdlegf gdrrllrksa savlnqriga ngdedasvig krrrltngqs gsssrrvkgs
      901 skiaeegfat vngsigdkkn fgaetnaeda vegrgfreda svdvnrsnes nivvngtaen
      961 vtmqqriftt vpsspsgqei idsttpqtpe ntteedrsns tetvtlmnrh aeidtkdknt
     1021 vfrsendsvp vnneshgnst ggttngtesg ngsptvtvta tvdettvlke tastitpipa
     1081 pvfpvtprii tevdraplek etftskptef anqvtdiktt vpnepldaan ldpseiqkmy
     1141 rskdatttat sttevptttm tavqieiati pqdqsttset shvksetpts ppivesrkrv
     1201 agerspevrp rnlsdeskdk ddvlpimhly ntsdifngsg pmnslprgte rraelpvdpk
     1261 lpevsrttek andtvtpknv tndfstrvpv sestrkdkhd daretfsqvp ptgpgrgsmn
     1321 gngnnrtrhr pvyhhtilpe yntsvynnel nrtffetgli svspresvni seviskrhdg
     1381 daiasqetva vvsyilatlv vfpiavgvgl ilrrlilrnr kvleesdtss eiscrkdaln
     1441 lengdfktsi ekaitklpri qhlcheaekp ppppsqesrw efprdklrlq tvlgqgnfgq
     1501 vwkaeaddlt ghqgttrlva vktvkegase rekedlvrel eimqqlgshp nvvtllgcct
     1561 eqephylile yvmygkllay lrdhrtrqdf ynfsedsaal tsrdltvfgy cvargmeyla
     1621 skkiihrdla arnvlvdhnk lckiadfgms rfanedgevi etrhgrnalp irwmapesli
     1681 yslfttktdv wsfgilmwei vtlgstpypd mtarevmrnv qngyrlerps hcrselfrvi
     1741 srcwhadpdr rpefqtlrrd laqllednln ghyvdlesfa sectd
//
LOCUS       XP_034183322            1115 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605753 [Osmia lignaria lignaria].
ACCESSION   XP_034183322
VERSION     XP_034183322.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327431.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1115
                     /product="uncharacterized protein LOC117605753"
                     /calculated_mol_wt=124367
     Region          <871..>1092
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..1115
                     /gene="LOC117605753"
                     /coded_by="XM_034327431.2:410..3757"
                     /db_xref="GeneID:117605753"
ORIGIN      
        1 mcdlidlksp dkkgllnsrl asplipvptd sagdncnnri ndsipvitsk rdslennpfd
       61 mvlhktteyi qkrddpfevt lekalrpkck kinelrthsf dftndynike knhvqklkmn
      121 ktldefline elnqktatsn kisnenvmvd snnvgaddvi ptinveevdl silnqslmnd
      181 tlfetdkeln kneiaplhnk ismqaekdkf nigqtipsnl llkfprlqrs lsqgenessk
      241 ksqlfkhlsl vealrtssen gsnisdrnsl dflnegflks yysgsslfss lsnvssiakl
      301 nfvssstsls ilspndtnnr aflescsfek sensrsignt dlnkeitsvt svptkctmsg
      361 lidqfdrlka klselpeslv eqknekccte nklidvdvfa pkvnsskecy rssvsdtssd
      421 svfldgskvn ksifheakll aktfeelamk tssgsstddl issnplwase llpafddedm
      481 vdnlielpts pnanhteskd ikvnecttte icaekdtevv inkmtenlke leleliesvh
      541 kekhitaatl lselkklikt ennpeanklv enlekvlgid fenntelltt ylnttnnlak
      601 spqksdsslk viqnieknnm ehsqednlsn dsdnvkespv ykniniddsn inkcitnqkc
      661 ldkinsdspk inteetnknr nekelnieie ckedsskkgs nnllnekvvi elltnigkll
      721 tgqtetqpts diltnlgkvf nfaanddnmk iqqtpkkpkl kfenkthsli sstshrqsln
      781 lepkkqslhk nfirrsisvs htsatkdpvp itssnnsecq lkevtkrfps dpgfvsptsn
      841 kkvvtknnkd glkkdtqtkt latldlqkek tatintvknk lkkkidtevt nkkgpmkaml
      901 pigtmqkkkt gnskvtssyg tvtppkshki isstpnsprn dcamkrssrs skpvasstpd
      961 aynsksrkvq spqvakkrnf scdispvsir tgvdnkinss pkrklsspkk ttpkrrsvgs
     1021 gipksqtppv tkklnssfdv nqysklyesp qrssyktsns qnsspvsvkk nesvqqsllr
     1081 dnnkliykak pmklisklqr hsvdtnvaek ennyi
//
LOCUS       XP_034183323             669 aa            linear   INV 03-SEP-2025
DEFINITION  L(3)mbt interactor in ovarian somatic cells isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183323
VERSION     XP_034183323.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327432.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..669
                     /product="L(3)mbt interactor in ovarian somatic cells
                     isoform X1"
                     /calculated_mol_wt=75922
     Site            order(438,450..453,457,479)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          439..487
                     /region_name="PHD"
                     /note="PHD-finger; pfam00628"
                     /db_xref="CDD:425785"
     Site            order(486,499..502,506,527)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          487..532
                     /region_name="PHD"
                     /note="PHD zinc finger; smart00249"
                     /db_xref="CDD:214584"
     Region          595..662
                     /region_name="SAM_Atherin-like"
                     /note="SAM domain of Atherin/Atherin-like subfamily;
                     cd09583"
                     /db_xref="CDD:188982"
     Site            order(622..624,626..627,630..631,634..635,640)
                     /site_type="other"
                     /note="putative oligomer interface ML [polypeptide
                     binding]"
                     /db_xref="CDD:188982"
     Site            order(643..646,648..649,652,655)
                     /site_type="other"
                     /note="putative oligomer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188982"
     CDS             1..669
                     /gene="Lint-O"
                     /coded_by="XM_034327432.2:2180..4189"
                     /db_xref="GeneID:117605754"
ORIGIN      
        1 mqqhrvetqi lheeqileai dqlrrrkarp dadricnyll rkfsvdardt iadlhrliea
       61 ekviqvdykg ntsyrnaskw srlqlyknrp egfvkeklns gmvagavael vveepdyldq
      121 gvpayrlieq lldgvsnpts rrmvedflgk evasgnltrl sngnyslvat sdmtttvest
      181 hresftleng narrkeiqtv ssttgglydf detenltitd srsntprssr qaspksdpqv
      241 rkddcyeiiv grnenadllv ehdsirdqes ldpsqssheg tkeeskntdn qthnlkrssk
      301 aerkqrllvr tddpmdieik fedyrkdnke eihsqkekre esghlseere dedagrsstn
      361 psptpsntnv ggfrsarrkl skctktmfqr akkvfdpsdn nlvkrkrgrq pgsqnkssis
      421 qepqdsskst vkegpcrqcs lcakekqetl vacrdctvra hpsciyspee miqkagsswq
      481 cercksctic cetsdagpla tcftcdeayh yychtpriii pksnskwqcs dctqkqyktn
      541 isqhislvtn rpdtpsnppv lppvlspqvs pargssdqme ddsprdgidp nipdasdwts
      601 eqvyqyfarl fpkeaevfrq qdidghslll mkrsdvlsgl dlllgpalki yrhvlklqvr
      661 rddpklywl
//
LOCUS       XP_034183324             664 aa            linear   INV 03-SEP-2025
DEFINITION  L(3)mbt interactor in ovarian somatic cells isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183324
VERSION     XP_034183324.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327433.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..664
                     /product="L(3)mbt interactor in ovarian somatic cells
                     isoform X2"
                     /calculated_mol_wt=75384
     Site            order(433,445..448,452,474)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          434..482
                     /region_name="PHD"
                     /note="PHD-finger; pfam00628"
                     /db_xref="CDD:425785"
     Site            order(481,494..497,501,522)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          482..527
                     /region_name="PHD"
                     /note="PHD zinc finger; smart00249"
                     /db_xref="CDD:214584"
     Region          590..657
                     /region_name="SAM_Atherin-like"
                     /note="SAM domain of Atherin/Atherin-like subfamily;
                     cd09583"
                     /db_xref="CDD:188982"
     Site            order(617..619,621..622,625..626,629..630,635)
                     /site_type="other"
                     /note="putative oligomer interface ML [polypeptide
                     binding]"
                     /db_xref="CDD:188982"
     Site            order(638..641,643..644,647,650)
                     /site_type="other"
                     /note="putative oligomer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188982"
     CDS             1..664
                     /gene="Lint-O"
                     /coded_by="XM_034327433.2:2180..4174"
                     /db_xref="GeneID:117605754"
ORIGIN      
        1 mqqhrvetqi lheeqileai dqlrrrkarp dadricnyll rkfsvdardt iadlhrliea
       61 ekviqvdykg ntsyrnaskw srlqlyknrp egfvkeklns gmvagavael vveepdyldq
      121 gvpayrlieq lldgvsnpts rrmvedflgk evasgnltrl sngnyslvat sdmtttvest
      181 hresftleng narrkeiqtv ssttgglydf detenltitd srsntprssr qaspksdpqv
      241 rkddcyeiiv grnenadllv ehdsirdqes ldpsqssheg tkeeskntdn qthnlkrssk
      301 aerkqrllvr tddpmdieik fedyrkdnke eihsqkekre esghlseere dedagrsstn
      361 psptpsntnv ggfrsarrkv rviyrakkvf dpsdnnlvkr krgrqpgsqn kssisqepqd
      421 sskstvkegp crqcslcake kqetlvacrd ctvrahpsci yspeemiqka gsswqcerck
      481 scticcetsd agplatcftc deayhyycht priiipksns kwqcsdctqk qyktnisqhi
      541 slvtnrpdtp snppvlppvl spqvspargs sdqmeddspr dgidpnipda sdwtseqvyq
      601 yfarlfpkea evfrqqdidg hslllmkrsd vlsgldlllg palkiyrhvl klqvrrddpk
      661 lywl
//
LOCUS       XP_034183325             659 aa            linear   INV 03-SEP-2025
DEFINITION  L(3)mbt interactor in ovarian somatic cells isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183325
VERSION     XP_034183325.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327434.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..659
                     /product="L(3)mbt interactor in ovarian somatic cells
                     isoform X3"
                     /calculated_mol_wt=74754
     Site            order(428,440..443,447,469)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          429..477
                     /region_name="PHD"
                     /note="PHD-finger; pfam00628"
                     /db_xref="CDD:425785"
     Site            order(476,489..492,496,517)
                     /site_type="other"
                     /note="histone H3 binding site [polypeptide binding]"
                     /db_xref="CDD:276966"
     Region          477..522
                     /region_name="PHD"
                     /note="PHD zinc finger; smart00249"
                     /db_xref="CDD:214584"
     Region          585..652
                     /region_name="SAM_Atherin-like"
                     /note="SAM domain of Atherin/Atherin-like subfamily;
                     cd09583"
                     /db_xref="CDD:188982"
     Site            order(612..614,616..617,620..621,624..625,630)
                     /site_type="other"
                     /note="putative oligomer interface ML [polypeptide
                     binding]"
                     /db_xref="CDD:188982"
     Site            order(633..636,638..639,642,645)
                     /site_type="other"
                     /note="putative oligomer interface EH [polypeptide
                     binding]"
                     /db_xref="CDD:188982"
     CDS             1..659
                     /gene="Lint-O"
                     /coded_by="XM_034327434.2:2170..4149"
                     /db_xref="GeneID:117605754"
ORIGIN      
        1 mqqhrvetqi lheeqileai dqlrrrkarp dadricnyll rkfsvdardt iadlhrliea
       61 ekviqvdykg ntsyrnaskw srlqlyknrp egfvkeklns gmvagavael vveepdyldq
      121 gvpayrlieq lldgvsnpts rrmvedflgk evasgnltrl sngnyslvat sdmtttvest
      181 hresftleng narrkeiqtv ssttgglydf detenltitd srsntprssr qaspksdpqv
      241 rkddcyeiiv grnenadllv ehdsirdqes ldpsqssheg tkeeskntdn qthnlkrssk
      301 aerkqrllvr tddpmdieik fedyrkdnke eihsqkekre esghlseere dedagrsstn
      361 psptpsntnv ggfrsarrkr akkvfdpsdn nlvkrkrgrq pgsqnkssis qepqdsskst
      421 vkegpcrqcs lcakekqetl vacrdctvra hpsciyspee miqkagsswq cercksctic
      481 cetsdagpla tcftcdeayh yychtpriii pksnskwqcs dctqkqyktn isqhislvtn
      541 rpdtpsnppv lppvlspqvs pargssdqme ddsprdgidp nipdasdwts eqvyqyfarl
      601 fpkeaevfrq qdidghslll mkrsdvlsgl dlllgpalki yrhvlklqvr rddpklywl
//
LOCUS       XP_034183328             289 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 59-like [Osmia lignaria lignaria].
ACCESSION   XP_034183328
VERSION     XP_034183328.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327437.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="transmembrane protein 59-like"
                     /calculated_mol_wt=32137
     Region          57..218
                     /region_name="BSMAP"
                     /note="Brain specific membrane anchored protein;
                     pfam12280"
                     /db_xref="CDD:463519"
     CDS             1..289
                     /gene="LOC117605756"
                     /coded_by="XM_034327437.2:567..1436"
                     /db_xref="GeneID:117605756"
ORIGIN      
        1 mrkekeivsl lllllvnvah sdmfynllnr edpcinlcek tplsftnsky vksccqrgcr
       61 ffnlvdlhyg lepnslngtr dsceasctea ytapqdryac stgcdfmakq rlsdilslfs
      121 favyveegvn snivlmspdi pendiltdpg lrkellpgww dsngfklpqt yiktvpldag
      181 tvdygvpsdy fgendqsasi pgsdwlqcas khiglphwll asaiaaaals afwlclypek
      241 ptdpykeili eksdisptia lyipeaplhk kpppkysecv dttesnmkv
//
LOCUS       XP_034190706             814 aa            linear   INV 03-SEP-2025
DEFINITION  M13 family metallopeptidase neprilysin 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190706
VERSION     XP_034190706.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334815.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..814
                     /product="M13 family metallopeptidase neprilysin 2 isoform
                     X1"
                     /calculated_mol_wt=92241
     Region          156..811
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(186,600,607..610,629,645..646,649..650,653,711,754,
                     757..758,775..776,782)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..814
                     /gene="Nep2"
                     /coded_by="XM_034334815.2:258..2702"
                     /db_xref="GeneID:117609002"
ORIGIN      
        1 mnseepnfan trtlhfqnld isenvkeesp rsergnifdn vykltttdrn ptwwkrrsal
       61 ergltviavs gvlvcialav algvlaanta scssaarnep vnteglpsta dalngyqssk
      121 diqivdrgpp cdenvcytpe cihtasrilk nmdpevepcd dfydfacggf lkstnipddk
      181 tsvntfteis delqnqlrts ieeksppnep ksfrlaknly kacmnktvie qqgldpliki
      241 leklggwpvl egddwnetef swtdsvyrfr kmgysvdyfi dfsigvdlkn stqriidldq
      301 aslglsreyl skgfndkivq ayysymvdia tilganktta kselkeslef eiklanislp
      361 nekrrnatll ynpmtvrels ltyssvpwke yfntllapnv evtedevviv svpsyianle
      421 klldtvpkrv qanyvmwraa assvsyltde irkrqlqyst alsgkterep rwkecidtvs
      481 gslaisvgam yvrkyfkeda kknalemvad ireefteilk kvdwmdddtr ksalkkaasm
      541 sshiaypdel lddkkleefy eklelttdny legilnltlf gveysfsklr kpvnksdwvt
      601 hgrpaivnaf yssiensiqf pagilqgaff sndrprymny gaigfvighe ithgfddqgr
      661 qfdkdgnlvd wwapqtkeny leraeciihq ygnytvedvg lnlngintqg eniadnggik
      721 eaylayrewv krnkpeqklp gmsfspeqlf wisaantwcs kyrpeamklr ittgfhspgk
      781 frvlgplsnm eefskdfncp lgskmnpekk cavw
//
LOCUS       XP_034190707             803 aa            linear   INV 03-SEP-2025
DEFINITION  M13 family metallopeptidase neprilysin 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190707
VERSION     XP_034190707.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334816.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..803
                     /product="M13 family metallopeptidase neprilysin 2 isoform
                     X2"
                     /calculated_mol_wt=91115
     Region          145..800
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(175,589,596..599,618,634..635,638..639,642,700,743,
                     746..747,764..765,771)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..803
                     /gene="Nep2"
                     /coded_by="XM_034334816.2:258..2669"
                     /db_xref="GeneID:117609002"
ORIGIN      
        1 mnseepnfan trtlhfqnld isenvkeesp rsergnifdn vykltttdrn ptwwkrrsal
       61 ergltviavs gvlvcialav algvlaanta scssaarnad alngyqsskd iqivdrgppc
      121 denvcytpec ihtasrilkn mdpevepcdd fydfacggfl kstnipddkt svntfteisd
      181 elqnqlrtsi eeksppnepk sfrlaknlyk acmnktvieq qgldplikil eklggwpvle
      241 gddwnetefs wtdsvyrfrk mgysvdyfid fsigvdlkns tqriidldqa slglsreyls
      301 kgfndkivqa yysymvdiat ilgankttak selkeslefe iklanislpn ekrrnatlly
      361 npmtvrelsl tyssvpwkey fntllapnve vtedevvivs vpsyianlek lldtvpkrvq
      421 anyvmwraaa ssvsyltdei rkrqlqysta lsgkterepr wkecidtvsg slaisvgamy
      481 vrkyfkedak knalemvadi reefteilkk vdwmdddtrk salkkaasms shiaypdell
      541 ddkkleefye klelttdnyl egilnltlfg veysfsklrk pvnksdwvth grpaivnafy
      601 ssiensiqfp agilqgaffs ndrprymnyg aigfvighei thgfddqgrq fdkdgnlvdw
      661 wapqtkenyl eraeciihqy gnytvedvgl nlngintqge niadnggike aylayrewvk
      721 rnkpeqklpg msfspeqlfw isaantwcsk yrpeamklri ttgfhspgkf rvlgplsnme
      781 efskdfncpl gskmnpekkc avw
//
LOCUS       XP_034190709             768 aa            linear   INV 03-SEP-2025
DEFINITION  M13 family metallopeptidase neprilysin 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190709
VERSION     XP_034190709.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334818.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..768
                     /product="M13 family metallopeptidase neprilysin 2 isoform
                     X3"
                     /calculated_mol_wt=86819
     Region          110..765
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(140,554,561..564,583,599..600,603..604,607,665,708,
                     711..712,729..730,736)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..768
                     /gene="Nep2"
                     /coded_by="XM_034334818.2:16..2322"
                     /db_xref="GeneID:117609002"
ORIGIN      
        1 mtsnptwwkr rsalergltv iavsgvlvci alavalgvla antascssaa rnepvntegl
       61 pstadalngy qsskdiqivd rgppcdenvc ytpecihtas rilknmdpev epcddfydfa
      121 cggflkstni pddktsvntf teisdelqnq lrtsieeksp pnepksfrla knlykacmnk
      181 tvieqqgldp likileklgg wpvlegddwn etefswtdsv yrfrkmgysv dyfidfsigv
      241 dlknstqrii dldqaslgls reylskgfnd kivqayysym vdiatilgan kttakselke
      301 slefeiklan islpnekrrn atllynpmtv relsltyssv pwkeyfntll apnvevtede
      361 vvivsvpsyi anleklldtv pkrvqanyvm wraaassvsy ltdeirkrql qystalsgkt
      421 ereprwkeci dtvsgslais vgamyvrkyf kedakknale mvadireeft eilkkvdwmd
      481 ddtrksalkk aasmsshiay pdellddkkl eefyeklelt tdnylegiln ltlfgveysf
      541 sklrkpvnks dwvthgrpai vnafyssien siqfpagilq gaffsndrpr ymnygaigfv
      601 igheithgfd dqgrqfdkdg nlvdwwapqt kenyleraec iihqygnytv edvglnlngi
      661 ntqgeniadn ggikeaylay rewvkrnkpe qklpgmsfsp eqlfwisaan twcskyrpea
      721 mklrittgfh spgkfrvlgp lsnmeefskd fncplgskmn pekkcavw
//
LOCUS       XP_034190710             776 aa            linear   INV 03-SEP-2025
DEFINITION  M13 family metallopeptidase neprilysin 2 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190710
VERSION     XP_034190710.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334819.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..776
                     /product="M13 family metallopeptidase neprilysin 2 isoform
                     X4"
                     /calculated_mol_wt=87762
     Region          118..773
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(148,562,569..572,591,607..608,611..612,615,673,716,
                     719..720,737..738,744)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..776
                     /gene="Nep2"
                     /coded_by="XM_034334819.2:284..2614"
                     /db_xref="GeneID:117609002"
ORIGIN      
        1 mtnsmkqtvi knptwwkrrs alergltvia vsgvlvcial avalgvlaan tascssaarn
       61 epvnteglps tadalngyqs skdiqivdrg ppcdenvcyt pecihtasri lknmdpevep
      121 cddfydfacg gflkstnipd dktsvntfte isdelqnqlr tsieeksppn epksfrlakn
      181 lykacmnktv ieqqgldpli kileklggwp vlegddwnet efswtdsvyr frkmgysvdy
      241 fidfsigvdl knstqriidl dqaslglsre ylskgfndki vqayysymvd iatilgankt
      301 takselkesl efeiklanis lpnekrrnat llynpmtvre lsltyssvpw keyfntllap
      361 nvevtedevv ivsvpsyian leklldtvpk rvqanyvmwr aaassvsylt deirkrqlqy
      421 stalsgkter eprwkecidt vsgslaisvg amyvrkyfke dakknalemv adireeftei
      481 lkkvdwmddd trksalkkaa smsshiaypd ellddkklee fyeklelttd nylegilnlt
      541 lfgveysfsk lrkpvnksdw vthgrpaivn afyssiensi qfpagilqga ffsndrprym
      601 nygaigfvig heithgfddq grqfdkdgnl vdwwapqtke nyleraecii hqygnytved
      661 vglnlngint qgeniadngg ikeaylayre wvkrnkpeqk lpgmsfspeq lfwisaantw
      721 cskyrpeamk lrittgfhsp gkfrvlgpls nmeefskdfn cplgskmnpe kkcavw
//
LOCUS       XP_034190711             256 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609003 [Osmia lignaria lignaria].
ACCESSION   XP_034190711
VERSION     XP_034190711.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334820.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="uncharacterized protein LOC117609003"
                     /calculated_mol_wt=29246
     Region          <24..>248
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          <27..>88
                     /region_name="flgK"
                     /note="flagellar hook-associated protein FlgK; Validated;
                     PRK08471"
                     /db_xref="CDD:236270"
     CDS             1..256
                     /gene="LOC117609003"
                     /coded_by="XM_034334820.2:207..977"
                     /db_xref="GeneID:117609003"
ORIGIN      
        1 msfskaetqr fnevendvvp vpgecdqkvq tldhlisnya eliikmeklv eddrnemsll
       61 aqniselnsr ivdlsderdk lelkvqklmv iteavaretl aayraecner anamirevkt
      121 aiqaevvqvk kegvalnekl sqdaeilqss itleqlrsct krqesfllel aeqkeklsvl
      181 kdlirtlvte ltrskrfeii maanlasfst sikrlaqris egerlehelk rdedyinklr
      241 nllnivrnns tnecip
//
LOCUS       XP_034190712            1090 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609004 [Osmia lignaria lignaria].
ACCESSION   XP_034190712
VERSION     XP_034190712.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334821.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190712.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1090
                     /product="uncharacterized protein LOC117609004"
                     /calculated_mol_wt=121494
     Region          461..486
                     /region_name="zf-C2H2_jaz"
                     /note="Zinc-finger double-stranded RNA-binding; pfam12171"
                     /db_xref="CDD:432381"
     Region          970..>1008
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          972..993
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          972..993
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(977,983,990,992,1000,1007)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          1001..1021
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(1006,1008,1010,1012..1013,1016..1017,1020,1034,1036,
                     1040..1041,1044..1045,1048,1062,1064,1066,1068..1069,
                     1072..1073,1076)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          1027..1049
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          1029..1049
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          1041..1066
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          1057..1077
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..1090
                     /gene="LOC117609004"
                     /coded_by="XM_034334821.2:1466..4738"
                     /db_xref="GeneID:117609004"
ORIGIN      
        1 msemtsveqq qqqqqyvggs snpehhckdc glyfesdksl evhlryhqen llsqwasqaq
       61 qeesnnnnsk agnhnshvgv kresvtapad ssesssrpps qdptssqqsq qqqhsqqqqq
      121 qqqqqqqqpq qqqpggqnyn hfqasmfgea nyfmqnepay ilphhysppr edaqnnggss
      181 gggggggysr yhpyqhqqhf ppertssvss tsprsppiqc dkcgavyeda nqlgehvrtn
      241 hsnspsgypg qqqyqqlgns pqqqnqqqql hssppqsgqq qqqqqqqsgy dynggqtmkt
      301 elkqepeeqa eildldshkv qthryeeelm rlhqqqqqel qmqmhqqqvm qqhqqqqrag
      361 shsvssmlgw ppaaqphdyh pglspmgpmd svsplsdqgq fmrgqhmpve pprhpgspii
      421 tstqpmpghq mpggllqqpp kppplanqsw ksnearrpkt ynctacnkwf tssghlkrhy
      481 nttlhknavk qsnqpdpanm pisahhhpgr dshsgggrgg gpsrspelss sgsppnlmag
      541 psgeaargll htpttlynnn nssnnnnnsn hsnssssses saaltqqqqq qqqapsgvhl
      601 gstmvpplns paqsplaahh qqqmgspspq lghqqqqqql hhlpmgspsg qlpvhhpmss
      661 pmspmhpppa phlsspspmg sshphhmssp spitpgsvhp amasptamgg ttmphqpypn
      721 alpphvtttt nipgllesit iqltttgnem plplsgqsqh qqsqhhhhhq qqqqqqqqqq
      781 qqqhhqqqqq qqqqqqtqdv lpgfgtfsnh qrplpsftqf nvtgflighn qpqavnvggl
      841 speenasphe rsfdssesky dpyrssppry esltnmdvnm hpntdgmiik qyevhthnhh
      901 msnsihqspd nleannnlpq aiqakeelnp nigryyqkhq kskvnsvitk ehqvtstntg
      961 shyisqdgfh kciecdkvfn kacyltqhnk sfhsgdkpfk cnqcgkrfpl eylhaehlqk
     1021 hagdkpykce icpkqfnhkt dlrrhmclht gekpyacdnc gkgfirkdhm mkhlethkkk
     1081 snnhnvhlra
//
LOCUS       XP_034191285            1620 aa            linear   INV 03-SEP-2025
DEFINITION  glutamyl-prolyl-tRNA synthetase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191285
VERSION     XP_034191285.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335394.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191285.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1620
                     /product="glutamyl-prolyl-tRNA synthetase isoform X1"
                     /calculated_mol_wt=185271
     Region          47..731
                     /region_name="PLN02907"
                     /note="glutamate-tRNA ligase"
                     /db_xref="CDD:215492"
     Region          753..805
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          880..926
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          954..1006
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          1021..1072
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          1120..1620
                     /region_name="proS_fam_I"
                     /note="prolyl-tRNA synthetase, family I; TIGR00408"
                     /db_xref="CDD:273062"
     CDS             1..1620
                     /gene="GluProRS"
                     /coded_by="XM_034335394.2:120..4982"
                     /db_xref="GeneID:117609270"
ORIGIN      
        1 mnfklllhsk npstealiva evinsqkkdk ieivwcndwk gdgnvqlidd dnkvlgewsn
       61 disryfarts yiqlyggtsp cektevdhwl sfaigplgfv plkksalqyl ddvlmtttwl
      121 vskkltladi hvfcalldke flpkfekqfi nitrwykqml slpviaqvll svkknaklpv
      181 qsekvvvnkq sgqrkqegkf idlpgaemgk vivrfppeas gylhighaka allnqyyaea
      241 fqgqlimrfd dtnpakenve fekailedle llqikpdrft hssdyfdlml eyctkllien
      301 kayvddtpah imkeqrdqkl msvnrnnsve knlklwnemq qgspkgqecc vrakidyqsa
      361 ngclrdptiy rckpephprt gtkykvypty dfacpivdav enithtlrtt eyhdrdvqfy
      421 wiidalglrr pyiweysrln mtntvlskrk ltwfvneglv dgwddprfpt vrgilrrgmt
      481 veglkqfiia qgssrsvvfm ewdkiwsfnk kvidpiatry taldfdklvs vhinnvkeew
      541 ltvqnhpkdp srgnkqvlts shlyierdda dvlvegqnat finwgniliq kvekengvvk
      601 riiaqlnlen kdykntlkit wlavpsnkdd ddsktnlipc yavyfehiir kpvlgkdddf
      661 knfiakdtrv evqmlgevel krvkkgeiiq lqrkgffrcd vpyvsaspfs skeqplilfh
      721 vpdghtissa ktavkettqk nvsqlevqda nvisekiiaq gdkvrslksq kadktvidae
      781 vkvllnlkae yeaytgvewk pgmipdksdk iapsdknkls ediiqqekki kelqakinqe
      841 mqylsnlkts yknkigsdws ganeniaqng klpdshaekl sneikkqgdk vrelkksqvd
      901 ksiidveikk lltlkgdyks itgqdwkqss tstsktpska cdnvinkmtd hdkiaeeiqk
      961 qgdkirqlka tkaekpiidq evkllltlka dyktatgkdw kpfntpvana snkeeirvnq
     1021 ilesiqkqed kvrqlneska ekniidqevk ilfdlkndyk tltgqewksv kpdtstkkkk
     1081 kenisktekr kgtpnkeisk sestkdtdts ktgtrlglea kkaenffewy sqlitksgmi
     1141 eyydvsgcyi frpwsfsvwk iikgyidaei tklgvqecyf pmfvtrsvle kekahiadfa
     1201 pevawvtkcg esdlaepiai rptsetvmyp ayakwlrsdt elplrlnqwn nvvrwefkdp
     1261 kpflrtrefl wqeghsafat kaeadeevlv ildvyakvye hllaipvikg rktekekfag
     1321 gdytttveaf ismsgraiqg atshhlgqnf skmfniqieg ategdektfv yqnswglttr
     1381 tigvmimvhg ddkglvlppn vaciqaiivp cgitanttsq qreflftecd klldelsrdk
     1441 klrvkadyrs nrtpgwkfnh welkgvpvri elgpkdlekn qvtfvrrdns erilanrsev
     1501 lnalytlldn iqssmltkar kelnehikra dnwnefcvel nkknlllspf cgesscedki
     1561 kadsaredag eepgmlsmga kslcipfeqp tssvplhkqk cihpdcqnkp ksytlfgrsy
//
LOCUS       XP_034191286            1374 aa            linear   INV 03-SEP-2025
DEFINITION  glutamyl-prolyl-tRNA synthetase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191286
VERSION     XP_034191286.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335395.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191286.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1374
                     /product="glutamyl-prolyl-tRNA synthetase isoform X2"
                     /calculated_mol_wt=157401
     Region          <2..485
                     /region_name="PLN02907"
                     /note="glutamate-tRNA ligase"
                     /db_xref="CDD:215492"
     Region          507..559
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          634..680
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          708..760
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          775..826
                     /region_name="WHEP-TRS"
                     /note="WHEP-TRS domain; pfam00458"
                     /db_xref="CDD:459819"
     Region          874..1374
                     /region_name="proS_fam_I"
                     /note="prolyl-tRNA synthetase, family I; TIGR00408"
                     /db_xref="CDD:273062"
     CDS             1..1374
                     /gene="GluProRS"
                     /coded_by="XM_034335395.2:259..4383"
                     /db_xref="GeneID:117609270"
ORIGIN      
        1 mrfddtnpak envefekail edlellqikp drfthssdyf dlmleyctkl lienkayvdd
       61 tpahimkeqr dqklmsvnrn nsveknlklw nemqqgspkg qeccvrakid yqsangclrd
      121 ptiyrckpep hprtgtkykv yptydfacpi vdavenitht lrtteyhdrd vqfywiidal
      181 glrrpyiwey srlnmtntvl skrkltwfvn eglvdgwddp rfptvrgilr rgmtveglkq
      241 fiiaqgssrs vvfmewdkiw sfnkkvidpi atrytaldfd klvsvhinnv keewltvqnh
      301 pkdpsrgnkq vltsshlyie rddadvlveg qnatfinwgn iliqkveken gvvkriiaql
      361 nlenkdyknt lkitwlavps nkddddsktn lipcyavyfe hiirkpvlgk dddfknfiak
      421 dtrvevqmlg evelkrvkkg eiiqlqrkgf frcdvpyvsa spfsskeqpl ilfhvpdght
      481 issaktavke ttqknvsqle vqdanvisek iiaqgdkvrs lksqkadktv idaevkvlln
      541 lkaeyeaytg vewkpgmipd ksdkiapsdk nklsediiqq ekkikelqak inqemqylsn
      601 lktsyknkig sdwsganeni aqngklpdsh aeklsneikk qgdkvrelkk sqvdksiidv
      661 eikklltlkg dyksitgqdw kqsststskt pskacdnvin kmtdhdkiae eiqkqgdkir
      721 qlkatkaekp iidqevklll tlkadyktat gkdwkpfntp vanasnkeei rvnqilesiq
      781 kqedkvrqln eskaekniid qevkilfdlk ndyktltgqe wksvkpdtst kkkkkenisk
      841 tekrkgtpnk eisksestkd tdtsktgtrl gleakkaenf fewysqlitk sgmieyydvs
      901 gcyifrpwsf svwkiikgyi daeitklgvq ecyfpmfvtr svlekekahi adfapevawv
      961 tkcgesdlae piairptset vmypayakwl rsdtelplrl nqwnnvvrwe fkdpkpflrt
     1021 reflwqeghs afatkaeade evlvildvya kvyehllaip vikgrkteke kfaggdyttt
     1081 veafismsgr aiqgatshhl gqnfskmfni qiegategde ktfvyqnswg lttrtigvmi
     1141 mvhgddkglv lppnvaciqa iivpcgitan ttsqqreflf tecdklldel srdkklrvka
     1201 dyrsnrtpgw kfnhwelkgv pvrielgpkd leknqvtfvr rdnserilan rsevlnalyt
     1261 lldniqssml tkarkelneh ikradnwnef cvelnkknll lspfcgessc edkikadsar
     1321 edageepgml smgakslcip feqptssvpl hkqkcihpdc qnkpksytlf grsy
//
LOCUS       XP_034191293            1119 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609273 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191293
VERSION     XP_034191293.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335402.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191293.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1119
                     /product="uncharacterized protein LOC117609273 isoform X1"
                     /calculated_mol_wt=126422
     Region          11..122
                     /region_name="ePHD_PHF7_G2E3_like"
                     /note="Extended PHD finger found in PHD finger protein 7
                     (PHF7) and G2/M phase-specific E3 ubiquitin-protein ligase
                     (G2E3); cd15669"
                     /db_xref="CDD:277139"
     Site            order(75,84..88,94,117)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277139"
     Region          232..285
                     /region_name="PHD_PHF7_G2E3_like"
                     /note="PHD finger found in PHD finger protein 7 (PHF7) and
                     G2/M phase-specific E3 ubiquitin-protein ligase (G2E3);
                     cd15496"
                     /db_xref="CDD:276971"
     Site            order(232,254..258,262,280)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276971"
     CDS             1..1119
                     /gene="LOC117609273"
                     /coded_by="XM_034335402.2:617..3976"
                     /db_xref="GeneID:117609273"
ORIGIN      
        1 msqrkmlskr ccfcglsded elkfgkiyeh adiithyycl llssnmeqrg ddnegilgfl
       61 iadiqkelrr gkrltctyck ksgatlgccn vkckrifhyp cglnagtlnq ffgefrsfci
      121 nhrpkqkids emkkeltiss kvmcyicydq vnpysitdti wapccnknaw fhrkcvqqla
      181 msagyffkcp lcndkksfqk amlqfgifip sqdaswelvp nafeellyry dqcdapichc
      241 pkgrkhtsfn akwelilcrt cgsqgihmac gqlkwanpiw ectecisilg kskkkissnv
      301 tesilqnnsd ledsdsdisv gkdspvsfis tpipdstpcv stikqrpgpr tfklrqlkas
      361 kelqnlqlgs sskqeidkev dpvlelesin ecslqstste essiietksl svsetkssin
      421 ssseyfkpqs palddtdiil dsdddttqit scgslestcr iatvnetekd gllqirnnit
      481 eyegsnalhn stspiikkll qtnnfkpnna ssfltnnvnd nsvkynsqyv vqsddfeeti
      541 elnkkfeksc pgffsnikit nvvslapeef eivssginqq kinnieyqnk mfdinlkrkf
      601 dkttldplnf lidepkkikt nnsiyeqklk ssyvsvnvkn tgnnvhnvst stsdenysqi
      661 nnnnekctst ntsnlqslrs ctqleqccsk nenllpkyra nikhlfeant lkgtsngqis
      721 tlsedvnksd qtddnikncd gdagtssaak srsgsltsdt kinctsefik sgglyseser
      781 srtseqwksr snikkttist nktsniqkdk ntgcdysrli peylrlcdlk frvynsnnlq
      841 milynkfsin inientatve kntrfepsaq tgvqqkfsei qneislnsks enilhnisfl
      901 cnkdkskcml sneqsktyhd dtkenmnpvi tvpcknitds llsnanlath nltvsinssn
      961 qnddrdttqc ihnapkviee nnkesseend nlindfdnif daftnsnnky vksinnvtrs
     1021 iqnltsscvk saitkknnyf ynstklvrha lfpenrtnck penkaksisa aqfkvsidlk
     1081 kvekfidnnp nlfskykken eqcfkeqnsi terinllnm
//
LOCUS       XP_034191295            1004 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609273 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191295
VERSION     XP_034191295.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335404.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191295.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1004
                     /product="uncharacterized protein LOC117609273 isoform X2"
                     /calculated_mol_wt=113262
     Region          117..170
                     /region_name="PHD_PHF7_G2E3_like"
                     /note="PHD finger found in PHD finger protein 7 (PHF7) and
                     G2/M phase-specific E3 ubiquitin-protein ligase (G2E3);
                     cd15496"
                     /db_xref="CDD:276971"
     Site            order(117,139..143,147,165)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:276971"
     CDS             1..1004
                     /gene="LOC117609273"
                     /coded_by="XM_034335404.2:137..3151"
                     /db_xref="GeneID:117609273"
ORIGIN      
        1 msfcinhrpk qkidsemkke ltisskvmcy icydqvnpys itdtiwapcc nknawfhrkc
       61 vqqlamsagy ffkcplcndk ksfqkamlqf gifipsqdas welvpnafee llyrydqcda
      121 pichcpkgrk htsfnakwel ilcrtcgsqg ihmacgqlkw anpiwectec isilgkskkk
      181 issnvtesil qnnsdledsd sdisvgkdsp vsfistpipd stpcvstikq rpgprtfklr
      241 qlkaskelqn lqlgssskqe idkevdpvle lesinecslq ststeessii etkslsvset
      301 kssinsssey fkpqspaldd tdiildsddd ttqitscgsl estcriatvn etekdgllqi
      361 rnniteyegs nalhnstspi ikkllqtnnf kpnnassflt nnvndnsvky nsqyvvqsdd
      421 feetielnkk fekscpgffs nikitnvvsl apeefeivss ginqqkinni eyqnkmfdin
      481 lkrkfdkttl dplnflidep kkiktnnsiy eqklkssyvs vnvkntgnnv hnvststsde
      541 nysqinnnne kctstntsnl qslrsctqle qccsknenll pkyranikhl feantlkgts
      601 ngqistlsed vnksdqtddn ikncdgdagt ssaaksrsgs ltsdtkinct sefiksggly
      661 sesersrtse qwksrsnikk ttistnktsn iqkdkntgcd ysrlipeylr lcdlkfrvyn
      721 snnlqmilyn kfsininien tatvekntrf epsaqtgvqq kfseiqneis lnsksenilh
      781 nisflcnkdk skcmlsneqs ktyhddtken mnpvitvpck nitdsllsna nlathnltvs
      841 inssnqnddr dttqcihnap kvieennkes seendnlind fdnifdaftn snnkyvksin
      901 nvtrsiqnlt sscvksaitk knnyfynstk lvrhalfpen rtnckpenka ksisaaqfkv
      961 sidlkkvekf idnnpnlfsk ykkeneqcfk eqnsiterin llnm
//
LOCUS       XP_034190904             567 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha3 subunit isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190904
VERSION     XP_034190904.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335013.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..567
                     /product="nicotinic acetylcholine receptor alpha3 subunit
                     isoform X1"
                     /calculated_mol_wt=64544
     Region          24..242
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Region          27..533
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Site            order(60,62,68..69,76,98,100,109..110,112,114..115,119,
                     121..122,126..128,130,140,142,144,148,170,239)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          149..163
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     CDS             1..567
                     /gene="nAChRa3"
                     /coded_by="XM_034335013.2:528..2231"
                     /db_xref="GeneID:117609114"
ORIGIN      
        1 mmkslvgimw ivlvlisgcs gnpdakrlyd dllsnynklv rpvvnvtdal tvkiklklsq
       61 lidvnlknqi mttnlwveqs wydyklkwdp keyggvemlh vpsdhiwrpd ivlynnadgn
      121 fevtlatkat lnytgrvewk ppaiykssce idveyfpfde qtcvmkfgsw tydgfqvdlr
      181 hideirgsnv vdigvdlsef ytsvewdile vpavrnekfy tccdepyldi tfnitmrrkt
      241 lfytvnliip cmgisfltvl vfylpsdsge kvslsisill sltvffllla eiipptslvv
      301 pllgkfvlft mildtfsicv tvvvlnvhfr spqthvmapw vrrvfihvlp rllvmrrynt
      361 sskrtdynsr pqyqidkrsm aghhgqrvmv rtcnglelrd pslfvetsas elvessvlfp
      421 sldsrdelhp releavnlgs acrihgspaa taapppqlpt eesvdalcnt lhhwhhcpel
      481 ykaiegirfi adhtkreeds trvkedwkyv amvldrlflw iftlavvvgt agiilqaptl
      541 yddripidvr lseiasttak phivtsl
//
LOCUS       XP_034190907             554 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha3 subunit isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190907
VERSION     XP_034190907.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335016.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..554
                     /product="nicotinic acetylcholine receptor alpha3 subunit
                     isoform X2"
                     /calculated_mol_wt=62970
     Region          24..242
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Region          27..520
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Site            order(60,62,68..69,76,98,100,109..110,112,114..115,119,
                     121..122,126..128,130,140,142,144,148,170,239)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          149..163
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     CDS             1..554
                     /gene="nAChRa3"
                     /coded_by="XM_034335016.2:527..2191"
                     /db_xref="GeneID:117609114"
ORIGIN      
        1 mmkslvgimw ivlvlisgcs gnpdakrlyd dllsnynklv rpvvnvtdal tvkiklklsq
       61 lidvnlknqi mttnlwveqs wydyklkwdp keyggvemlh vpsdhiwrpd ivlynnadgn
      121 fevtlatkat lnytgrvewk ppaiykssce idveyfpfde qtcvmkfgsw tydgfqvdlr
      181 hideirgsnv vdigvdlsef ytsvewdile vpavrnekfy tccdepyldi tfnitmrrkt
      241 lfytvnliip cmgisfltvl vfylpsdsge kvslsisill sltvffllla eiipptslvv
      301 pllgkfvlft mildtfsicv tvvvlnvhfr spqthvmapw vrrvfihvlp rllvmrrpqy
      361 qidkrsmagh hgqrvmvrtc nglelrdpsl fvetsaselv essvlfpsld srdelhprel
      421 eavnlgsacr ihgspaataa pppqlptees vdalcntlhh whhcpelyka iegirfiadh
      481 tkreedstrv kedwkyvamv ldrlflwift lavvvgtagi ilqaptlydd ripidvrlse
      541 iasttakphi vtsl
//
LOCUS       XP_034190908             497 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha3 subunit isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190908
VERSION     XP_034190908.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335017.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="nicotinic acetylcholine receptor alpha3 subunit
                     isoform X3"
                     /calculated_mol_wt=56735
     Region          1..463
                     /region_name="LIC"
                     /note="Cation transporter family protein; cl42365"
                     /db_xref="CDD:455710"
     Region          <1..172
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Site            order(6,28,30,39..40,42,44..45,49,51..52,56..58,60,70,72,
                     74,78,100,169)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          79..93
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     CDS             1..497
                     /gene="nAChRa3"
                     /coded_by="XM_034335017.2:159..1652"
                     /db_xref="GeneID:117609114"
ORIGIN      
        1 mttnlwveqs wydyklkwdp keyggvemlh vpsdhiwrpd ivlynnadgn fevtlatkat
       61 lnytgrvewk ppaiykssce idveyfpfde qtcvmkfgsw tydgfqvdlr hideirgsnv
      121 vdigvdlsef ytsvewdile vpavrnekfy tccdepyldi tfnitmrrkt lfytvnliip
      181 cmgisfltvl vfylpsdsge kvslsisill sltvffllla eiipptslvv pllgkfvlft
      241 mildtfsicv tvvvlnvhfr spqthvmapw vrrvfihvlp rllvmrrynt sskrtdynsr
      301 pqyqidkrsm aghhgqrvmv rtcnglelrd pslfvetsas elvessvlfp sldsrdelhp
      361 releavnlgs acrihgspaa taapppqlpt eesvdalcnt lhhwhhcpel ykaiegirfi
      421 adhtkreeds trvkedwkyv amvldrlflw iftlavvvgt agiilqaptl yddripidvr
      481 lseiasttak phivtsl
//
LOCUS       XP_034190909             291 aa            linear   INV 03-SEP-2025
DEFINITION  nicotinic acetylcholine receptor alpha3 subunit isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190909
VERSION     XP_034190909.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335018.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..291
                     /product="nicotinic acetylcholine receptor alpha3 subunit
                     isoform X4"
                     /calculated_mol_wt=33540
     Region          24..242
                     /region_name="LGIC_ECD_nAChR_proto_alpha-like"
                     /note="extracellular domain of nicotinic acetylcholine
                     receptor subunit alpha-like found in protostomia; cd19031"
                     /db_xref="CDD:349832"
     Site            order(60,62,68..69,76,98,100,109..110,112,114..115,119,
                     121..122,126..128,130,140,142,144,148,170,239)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349832"
     Region          149..163
                     /region_name="Cys-loop"
                     /note="Cys-loop [structural motif]"
                     /db_xref="CDD:349832"
     Region          241..>271
                     /region_name="LGIC_TM"
                     /note="transmembrane domain of Cys-loop
                     neurotransmitter-gated ion channels; cl38911"
                     /db_xref="CDD:476813"
     Region          245..265
                     /region_name="TM1 helix"
                     /note="TM1 helix [structural motif]"
                     /db_xref="CDD:349866"
     CDS             1..291
                     /gene="nAChRa3"
                     /coded_by="XM_034335018.2:530..1405"
                     /db_xref="GeneID:117609114"
ORIGIN      
        1 mmkslvgimw ivlvlisgcs gnpdakrlyd dllsnynklv rpvvnvtdal tvkiklklsq
       61 lidvnlknqi mttnlwveqs wydyklkwdp keyggvemlh vpsdhiwrpd ivlynnadgn
      121 fevtlatkat lnytgrvewk ppaiykssce idveyfpfde qtcvmkfgsw tydgfqvdlr
      181 hideirgsnv vdigvdlsef ytsvewdile vpavrnekfy tccdepyldi tfnitmrrkt
      241 lfytvnliip cmgisfltvl vfylpsdsge krkkfgreap irsikmdsrl g
//
LOCUS       XP_034190910             368 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609116 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190910
VERSION     XP_034190910.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335019.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190910.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..368
                     /product="uncharacterized protein LOC117609116 isoform X1"
                     /calculated_mol_wt=43457
     CDS             1..368
                     /gene="LOC117609116"
                     /coded_by="XM_034335019.2:34..1140"
                     /db_xref="GeneID:117609116"
ORIGIN      
        1 meeamnnwvi vlhsvfdvlh fkpivanlwd ketfnmqrmn eirikrsdvl lgylipfwii
       61 lyfmyekcln sllrrmripl lqrsriieav wdcgfcfgsi cylksstvkt inffseeqav
      121 thqelgvilh ksfylhragv eifcheawik gwanllfasf vmnpyqekwc tvvstfvfyk
      181 afdtivisic rillctshfi gkklpklfff lhclswiyly tlfvpklmlw testnhtrak
      241 lglwlwfiae cidsmwfrll wcgkathwle iclfppptqe aielagiqkr hrdslkklvn
      301 rtskkaelwq tlfcavaikk kikrireakh nettsltdyp eilsqtnake epmrnekene
      361 nengkgkl
//
LOCUS       XP_076548068             313 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609116 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548068
VERSION     XP_076548068.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691953.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..313
                     /product="uncharacterized protein LOC117609116 isoform X2"
                     /calculated_mol_wt=37148
     CDS             1..313
                     /gene="LOC117609116"
                     /coded_by="XM_076691953.1:34..975"
                     /db_xref="GeneID:117609116"
ORIGIN      
        1 meeamnnwvi vlhsvfdvlh fkpivanlwd ketfnmqrmn eirikrsdvl lgylipfwii
       61 lyfmyekcln sllrrmripl lqrsriieav wdcgfcfgsi cylksstvkt inffseeqav
      121 thqelgvilh ksfylhragv eifcheawik gwanllfasf vmnpyqekwc tvvstfvfyk
      181 afdtivisic rillctshfi gkklpklfff lhclswiyly tlfvpklmlw testnhtrak
      241 lglwlwfiae cidsmwfrll wcgkathwle iclfppptqe aielagiqkr hrdslkklvn
      301 rtskkaelwq tlf
//
LOCUS       XP_034190911             308 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609116 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190911
VERSION     XP_034190911.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190911.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="uncharacterized protein LOC117609116 isoform X3"
                     /calculated_mol_wt=36129
     CDS             1..308
                     /gene="LOC117609116"
                     /coded_by="XM_034335020.2:173..1099"
                     /db_xref="GeneID:117609116"
ORIGIN      
        1 mfdtfhlltq sllrrmripl lqrsriieav wdcgfcfgsi cylksstvkt inffseeqav
       61 thqelgvilh ksfylhragv eifcheawik gwanllfasf vmnpyqekwc tvvstfvfyk
      121 afdtivisic rillctshfi gkklpklfff lhclswiyly tlfvpklmlw testnhtrak
      181 lglwlwfiae cidsmwfrll wcgkathwle iclfppptqe aielagiqkr hrdslkklvn
      241 rtskkaelwq tlfcavaikk kikrireakh nettsltdyp eilsqtnake epmrnekene
      301 nengkgkl
//
LOCUS       XP_034190913             308 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609116 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190913
VERSION     XP_034190913.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335022.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190913.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..308
                     /product="uncharacterized protein LOC117609116 isoform X3"
                     /calculated_mol_wt=36129
     CDS             1..308
                     /gene="LOC117609116"
                     /coded_by="XM_034335022.2:164..1090"
                     /db_xref="GeneID:117609116"
ORIGIN      
        1 mfdtfhlltq sllrrmripl lqrsriieav wdcgfcfgsi cylksstvkt inffseeqav
       61 thqelgvilh ksfylhragv eifcheawik gwanllfasf vmnpyqekwc tvvstfvfyk
      121 afdtivisic rillctshfi gkklpklfff lhclswiyly tlfvpklmlw testnhtrak
      181 lglwlwfiae cidsmwfrll wcgkathwle iclfppptqe aielagiqkr hrdslkklvn
      241 rtskkaelwq tlfcavaikk kikrireakh nettsltdyp eilsqtnake epmrnekene
      301 nengkgkl
//
LOCUS       XP_034190914             301 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609116 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190914
VERSION     XP_034190914.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335023.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..301
                     /product="uncharacterized protein LOC117609116 isoform X4"
                     /calculated_mol_wt=35673
     CDS             1..301
                     /gene="LOC117609116"
                     /coded_by="XM_034335023.2:34..939"
                     /db_xref="GeneID:117609116"
ORIGIN      
        1 meeamnnwvi vlhsvfdvlh fkpivanlwd ketfnmqrmn eirikrsdvl lgylipfwii
       61 lyfmyekcln sllrrmripl lqrsriieav wdcgfcfgsi cylksstvkt inffseeqav
      121 thqelgvilh ksfylhragv eifcheawik gwanllfasf vmnpyqekwc tvvstfvfyk
      181 afdtivisic rillctshfi gkklpklfff lhclswiyly tlfvpklmlw testnhtrak
      241 lglwlwfiae cidsmwfrll wcgkathwle iclfppptqe aielagiqkr hrdslkkcss
      301 y
//
LOCUS       XP_034190917             257 aa            linear   INV 03-SEP-2025
DEFINITION  ER membrane protein complex subunit 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190917
VERSION     XP_034190917.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335026.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190917.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..257
                     /product="ER membrane protein complex subunit 3 isoform
                     X1"
                     /calculated_mol_wt=29409
     Region          <44..192
                     /region_name="EMC3_TMCO1"
                     /note="Integral membrane protein EMC3/TMCO1-like;
                     pfam01956"
                     /db_xref="CDD:396507"
     CDS             1..257
                     /gene="EMC3"
                     /coded_by="XM_034335026.2:266..1039"
                     /db_xref="GeneID:117609118"
ORIGIN      
        1 mhvhtyikcs svclvflllf clprkfvers kwygrvafrs khsrkkvelh qvqdsqvmir
       61 srllrengqy ipkmafisrr hffnneetgy fktqkrapvs qnpmtdpnmm tdmlkgnvtn
      121 vlpmvliggw inwmfsgfvt tkvpfpltlr fkpmlqrgie lvtldaawvs saswyflnvf
      181 glrsiytlvl gesnaadtsr llqdqvsgaa msmppdpkaa fksewealei yehnwalqgv
      241 eadligsqrt dtsesnk
//
LOCUS       XP_034190918             252 aa            linear   INV 03-SEP-2025
DEFINITION  ER membrane protein complex subunit 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190918
VERSION     XP_034190918.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335027.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..252
                     /product="ER membrane protein complex subunit 3 isoform
                     X2"
                     /calculated_mol_wt=28500
     Region          3..187
                     /region_name="EMC3_TMCO1"
                     /note="Integral membrane protein EMC3/TMCO1-like;
                     pfam01956"
                     /db_xref="CDD:396507"
     CDS             1..252
                     /gene="EMC3"
                     /coded_by="XM_034335027.2:136..894"
                     /db_xref="GeneID:117609118"
ORIGIN      
        1 maellldpni rgwvflpivv itflvgiirh yvsillasqk kvelhqvqds qvmirsrllr
       61 engqyipkma fisrrhffnn eetgyfktqk rapvsqnpmt dpnmmtdmlk gnvtnvlpmv
      121 liggwinwmf sgfvttkvpf pltlrfkpml qrgielvtld aawvssaswy flnvfglrsi
      181 ytlvlgesna adtsrllqdq vsgaamsmpp dpkaafksew ealeiyehnw alqgveadli
      241 gsqrtdtses nk
//
LOCUS       XP_076548069             222 aa            linear   INV 03-SEP-2025
DEFINITION  ER membrane protein complex subunit 3 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548069
VERSION     XP_076548069.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691954.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..222
                     /product="ER membrane protein complex subunit 3 isoform
                     X3"
                     /calculated_mol_wt=25136
     Region          <10..157
                     /region_name="EMC3_TMCO1"
                     /note="Integral membrane protein EMC3/TMCO1-like;
                     pfam01956"
                     /db_xref="CDD:396507"
     CDS             1..222
                     /gene="EMC3"
                     /coded_by="XM_076691954.1:95..763"
                     /db_xref="GeneID:117609118"
ORIGIN      
        1 mqgcndfkdk kvelhqvqds qvmirsrllr engqyipkma fisrrhffnn eetgyfktqk
       61 rapvsqnpmt dpnmmtdmlk gnvtnvlpmv liggwinwmf sgfvttkvpf pltlrfkpml
      121 qrgielvtld aawvssaswy flnvfglrsi ytlvlgesna adtsrllqdq vsgaamsmpp
      181 dpkaafksew ealeiyehnw alqgveadli gsqrtdtses nk
//
LOCUS       XP_034191003            1608 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609149 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191003
VERSION     XP_034191003.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335112.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191003.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1608
                     /product="uncharacterized protein LOC117609149 isoform X1"
                     /calculated_mol_wt=180781
     Region          137..327
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            209..214
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1555..1608
                     /region_name="KASH"
                     /note="Nuclear envelope localization domain; pfam10541"
                     /db_xref="CDD:463142"
     CDS             1..1608
                     /gene="LOC117609149"
                     /coded_by="XM_034335112.2:128..4954"
                     /db_xref="GeneID:117609149"
ORIGIN      
        1 mneysdhcka mkmsfslssl qplshgldkh gnagtaierp ynsltkkrkd pkqlysqrlw
       61 vnredckddf waairsnydy imdtnlidsc keangelswd eadvstqsws fkeissqfse
      121 lyswlrvlee liyckeenll dkslraahme elrrrayrrk lfneqagklv srapalkdev
      181 awrvdhlnak welveqimap verpvsnqqd msadfehevk clrkwlreme srlqplsfhm
      241 dwtlaeleek avehmvlqrd ieahgrivss vvklgdkvfa qqqeqqqqee reketrkeqr
      301 eppqalriar slerrwhllf lralewqchi etlvsrissk nvvpyrcssd sdeepvtkqp
      361 rlsrrhsrgs grespgqsar strsdrsnrs nrskrqkatr sryyedsaas kadlsdtdas
      421 senrsigdgs yfedelcqlc vmdaksegae npqetvnria sslgdqgeeg dieedavsee
      481 deedempptp dtvpmtnsta ieencdqidg qpagnveqsn snnlianepv krrrtteeva
      541 sfntsdrksk ncatfyfkhl dtdseqneea aiaaedssee ewtytssran dvseqretnv
      601 lvrldfgesq sndneqekmk kaekavresv lgmanveets ndtdassrre kdsddetknd
      661 ktsiqrlike veklvgeerr tgacktfppl ilddkgitnn hrakyarike wlklnsargh
      721 dgrstsqpld scdasgeytt eesdgerqsv ssedlqssva tyrrfegalg ctsqsvsqei
      781 fdeaertpin eahpmldsst pkvvmrskqk sngprpwsvs cisqignnan lnqtndpisq
      841 fsisetalhq liatpptksv sldatgsrks fnnststlle esiichdgrv vrnsslrrkk
      901 srlrkknlgr ksdsssegvn anqsagsdgs ssnqqrsprk msgsrgirti srdchgrltt
      961 lvksgsfsgc tshqqfster tivsdptppf rlpcctsvas tsetepeeqr nctrscftyd
     1021 enlldtvlsn kelcnqqlnv dsdvemnslg nnsfseqawd nyqekymsep yseapdveta
     1081 rrlldfgddy rnfldsqsdc assmsavpts splprsrmhh eivdttedsd sdvedirnvv
     1141 eksqsqltla enlfaranng tmpedcteve cacrenlrcl hallesvsns frsekymkqv
     1201 rgmlekwesl ttrveetqma salhreltal qtefkaahdr lfsyeialeq phvlderinr
     1261 ittelaalrd rkaamlalnv sthrlitdlg nsasliftal kdgvadlyrv wdetfqkgnq
     1321 qlcalqavqq fsirlaelqc alrrdkdtla vldvalqaga tsevassvrh varllsekqd
     1381 mncqngtvlk dssteevssg astkfgdssp isltqeggsl sdsgisdsgs eqelsererr
     1441 laalrrltrs lenqlapgse algqlwkrve dtetelrdlq kqcrelivrt aasvearask
     1501 rtsnsqvhhy sdkrkktspt emargnseri tmiaaksgat dagdpdnepg lpqswvwril
     1561 raalpfqlal valfcaacll ephcceaant lnlsltpqlr yvrgpppv
//
LOCUS       XP_034191004            1604 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609149 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191004
VERSION     XP_034191004.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335113.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191004.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1604
                     /product="uncharacterized protein LOC117609149 isoform X2"
                     /calculated_mol_wt=180286
     Region          133..323
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            205..210
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1551..1604
                     /region_name="KASH"
                     /note="Nuclear envelope localization domain; pfam10541"
                     /db_xref="CDD:463142"
     CDS             1..1604
                     /gene="LOC117609149"
                     /coded_by="XM_034335113.2:180..4994"
                     /db_xref="GeneID:117609149"
ORIGIN      
        1 mnhckamkms fslsslqpls hgldkhgnag taierpynsl tkkrkdpkql ysqrlwvnre
       61 dckddfwaai rsnydyimdt nlidsckean gelswdeadv stqswsfkei ssqfselysw
      121 lrvleeliyc keenlldksl raahmeelrr rayrrklfne qagklvsrap alkdevawrv
      181 dhlnakwelv eqimapverp vsnqqdmsad fehevkclrk wlremesrlq plsfhmdwtl
      241 aeleekaveh mvlqrdieah grivssvvkl gdkvfaqqqe qqqqeereke trkeqreppq
      301 alriarsler rwhllflral ewqchietlv srissknvvp yrcssdsdee pvtkqprlsr
      361 rhsrgsgres pgqsarstrs drsnrsnrsk rqkatrsryy edsaaskadl sdtdassenr
      421 sigdgsyfed elcqlcvmda ksegaenpqe tvnriasslg dqgeegdiee davseedeed
      481 empptpdtvp mtnstaieen cdqidgqpag nveqsnsnnl ianepvkrrr tteevasfnt
      541 sdrkskncat fyfkhldtds eqneeaaiaa edsseeewty tssrandvse qretnvlvrl
      601 dfgesqsndn eqekmkkaek avresvlgma nveetsndtd assrrekdsd detkndktsi
      661 qrlikevekl vgeerrtgac ktfpplildd kgitnnhrak yarikewlkl nsarghdgrs
      721 tsqpldscda sgeytteesd gerqsvssed lqssvatyrr fegalgctsq svsqeifdea
      781 ertpineahp mldsstpkvv mrskqksngp rpwsvscisq ignnanlnqt ndpisqfsis
      841 etalhqliat pptksvslda tgsrksfnns tstlleesii chdgrvvrns slrrkksrlr
      901 kknlgrksds ssegvnanqs agsdgsssnq qrsprkmsgs rgirtisrdc hgrlttlvks
      961 gsfsgctshq qfstertivs dptppfrlpc ctsvastset epeeqrnctr scftydenll
     1021 dtvlsnkelc nqqlnvdsdv emnslgnnsf seqawdnyqe kymsepysea pdvetarrll
     1081 dfgddyrnfl dsqsdcassm savptssplp rsrmhheivd ttedsdsdve dirnvveksq
     1141 sqltlaenlf aranngtmpe dctevecacr enlrclhall esvsnsfrse kymkqvrgml
     1201 ekweslttrv eetqmasalh reltalqtef kaahdrlfsy eialeqphvl derinritte
     1261 laalrdrkaa mlalnvsthr litdlgnsas liftalkdgv adlyrvwdet fqkgnqqlca
     1321 lqavqqfsir laelqcalrr dkdtlavldv alqagatsev assvrhvarl lsekqdmncq
     1381 ngtvlkdsst eevssgastk fgdsspislt qeggslsdsg isdsgseqel sererrlaal
     1441 rrltrslenq lapgsealgq lwkrvedtet elrdlqkqcr elivrtaasv earaskrtsn
     1501 sqvhhysdkr kktsptemar gnseritmia aksgatdagd pdnepglpqs wvwrilraal
     1561 pfqlalvalf caacllephc ceaantlnls ltpqlryvrg pppv
//
LOCUS       XP_034191006            1598 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609149 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191006
VERSION     XP_034191006.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335115.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191006.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1598
                     /product="uncharacterized protein LOC117609149 isoform X3"
                     /calculated_mol_wt=179602
     Region          127..317
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            199..204
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     Region          1545..1598
                     /region_name="KASH"
                     /note="Nuclear envelope localization domain; pfam10541"
                     /db_xref="CDD:463142"
     CDS             1..1598
                     /gene="LOC117609149"
                     /coded_by="XM_034335115.2:154..4950"
                     /db_xref="GeneID:117609149"
ORIGIN      
        1 mkmsfslssl qplshgldkh gnagtaierp ynsltkkrkd pkqlysqrlw vnredckddf
       61 waairsnydy imdtnlidsc keangelswd eadvstqsws fkeissqfse lyswlrvlee
      121 liyckeenll dkslraahme elrrrayrrk lfneqagklv srapalkdev awrvdhlnak
      181 welveqimap verpvsnqqd msadfehevk clrkwlreme srlqplsfhm dwtlaeleek
      241 avehmvlqrd ieahgrivss vvklgdkvfa qqqeqqqqee reketrkeqr eppqalriar
      301 slerrwhllf lralewqchi etlvsrissk nvvpyrcssd sdeepvtkqp rlsrrhsrgs
      361 grespgqsar strsdrsnrs nrskrqkatr sryyedsaas kadlsdtdas senrsigdgs
      421 yfedelcqlc vmdaksegae npqetvnria sslgdqgeeg dieedavsee deedempptp
      481 dtvpmtnsta ieencdqidg qpagnveqsn snnlianepv krrrtteeva sfntsdrksk
      541 ncatfyfkhl dtdseqneea aiaaedssee ewtytssran dvseqretnv lvrldfgesq
      601 sndneqekmk kaekavresv lgmanveets ndtdassrre kdsddetknd ktsiqrlike
      661 veklvgeerr tgacktfppl ilddkgitnn hrakyarike wlklnsargh dgrstsqpld
      721 scdasgeytt eesdgerqsv ssedlqssva tyrrfegalg ctsqsvsqei fdeaertpin
      781 eahpmldsst pkvvmrskqk sngprpwsvs cisqignnan lnqtndpisq fsisetalhq
      841 liatpptksv sldatgsrks fnnststlle esiichdgrv vrnsslrrkk srlrkknlgr
      901 ksdsssegvn anqsagsdgs ssnqqrsprk msgsrgirti srdchgrltt lvksgsfsgc
      961 tshqqfster tivsdptppf rlpcctsvas tsetepeeqr nctrscftyd enlldtvlsn
     1021 kelcnqqlnv dsdvemnslg nnsfseqawd nyqekymsep yseapdveta rrlldfgddy
     1081 rnfldsqsdc assmsavpts splprsrmhh eivdttedsd sdvedirnvv eksqsqltla
     1141 enlfaranng tmpedcteve cacrenlrcl hallesvsns frsekymkqv rgmlekwesl
     1201 ttrveetqma salhreltal qtefkaahdr lfsyeialeq phvlderinr ittelaalrd
     1261 rkaamlalnv sthrlitdlg nsasliftal kdgvadlyrv wdetfqkgnq qlcalqavqq
     1321 fsirlaelqc alrrdkdtla vldvalqaga tsevassvrh varllsekqd mncqngtvlk
     1381 dssteevssg astkfgdssp isltqeggsl sdsgisdsgs eqelsererr laalrrltrs
     1441 lenqlapgse algqlwkrve dtetelrdlq kqcrelivrt aasvearask rtsnsqvhhy
     1501 sdkrkktspt emargnseri tmiaaksgat dagdpdnepg lpqswvwril raalpfqlal
     1561 valfcaacll ephcceaant lnlsltpqlr yvrgpppv
//
LOCUS       XP_034191007            1514 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609149 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191007
VERSION     XP_034191007.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335116.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191007.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1514
                     /product="uncharacterized protein LOC117609149 isoform X4"
                     /calculated_mol_wt=170628
     Region          137..327
                     /region_name="SPEC"
                     /note="Spectrin repeats, found in several proteins
                     involved in cytoskeletal structure; family members include
                     spectrin, alpha-actinin and dystrophin; the spectrin
                     repeat forms a three helix bundle with the second helix
                     interrupted by proline in some sequences; cd00176"
                     /db_xref="CDD:238103"
     Site            209..214
                     /site_type="active"
                     /note="linker region [active]"
                     /db_xref="CDD:238103"
     CDS             1..1514
                     /gene="LOC117609149"
                     /coded_by="XM_034335116.2:128..4672"
                     /db_xref="GeneID:117609149"
ORIGIN      
        1 mneysdhcka mkmsfslssl qplshgldkh gnagtaierp ynsltkkrkd pkqlysqrlw
       61 vnredckddf waairsnydy imdtnlidsc keangelswd eadvstqsws fkeissqfse
      121 lyswlrvlee liyckeenll dkslraahme elrrrayrrk lfneqagklv srapalkdev
      181 awrvdhlnak welveqimap verpvsnqqd msadfehevk clrkwlreme srlqplsfhm
      241 dwtlaeleek avehmvlqrd ieahgrivss vvklgdkvfa qqqeqqqqee reketrkeqr
      301 eppqalriar slerrwhllf lralewqchi etlvsrissk nvvpyrcssd sdeepvtkqp
      361 rlsrrhsrgs grespgqsar strsdrsnrs nrskrqkatr sryyedsaas kadlsdtdas
      421 senrsigdgs yfedelcqlc vmdaksegae npqetvnria sslgdqgeeg dieedavsee
      481 deedempptp dtvpmtnsta ieencdqidg qpagnveqsn snnlianepv krrrtteeva
      541 sfntsdrksk ncatfyfkhl dtdseqneea aiaaedssee ewtytssran dvseqretnv
      601 lvrldfgesq sndneqekmk kaekavresv lgmanveets ndtdassrre kdsddetknd
      661 ktsiqrlike veklvgeerr tgacktfppl ilddkgitnn hrakyarike wlklnsargh
      721 dgrstsqpld scdasgeytt eesdgerqsv ssedlqssva tyrrfegalg ctsqsvsqei
      781 fdeaertpin eahpmldsst pkvvmrskqk sngprpwsvs cisqignnan lnqtndpisq
      841 fsisetalhq liatpptksv sldatgsrks fnnststlle esiichdgrv vrnsslrrkk
      901 srlrkknlgr ksdsssegvn anqsagsdgs ssnqqrsprk msgsrgirti srdchgrltt
      961 lvksgsfsgc tshqqfster tivsdptppf rlpcctsvas tsetepeeqr nctrscftyd
     1021 enlldtvlsn kelcnqqlnv dsdvemnslg nnsfseqawd nyqekymsep yseapdveta
     1081 rrlldfgddy rnfldsqsdc assmsavpts splprsrmhh eivdttedsd sdvedirnvv
     1141 eksqsqltla enlfaranng tmpedcteve cacrenlrcl hallesvsns frsekymkqv
     1201 rgmlekwesl ttrveetqma salhreltal qtefkaahdr lfsyeialeq phvlderinr
     1261 ittelaalrd rkaamlalnv sthrlitdlg nsasliftal kdgvadlyrv wdetfqkgnq
     1321 qlcalqavqq fsirlaelqc alrrdkdtla vldvalqaga tsevassvrh varllsekqd
     1381 mncqngtvlk dssteevssg astkfgdssp isltqeggsl sdsgisdsgs eqelsererr
     1441 laalrrltrs lenqlapgse algqlwkrve dtetelrdlq kqcrelivrt aasvearask
     1501 rtsnsqvhhy send
//
LOCUS       XP_034191008            1253 aa            linear   INV 03-SEP-2025
DEFINITION  tolloid-like protein 1 tolkin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191008
VERSION     XP_034191008.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335117.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191008.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1253
                     /product="tolloid-like protein 1 tolkin isoform X1"
                     /calculated_mol_wt=141924
     Region          346..541
                     /region_name="ZnMc_BMP1_TLD"
                     /note="Zinc-dependent metalloprotease; BMP1/TLD-like
                     subfamily. BMP1 (Bone morphogenetic protein 1) and TLD
                     (tolloid)-like metalloproteases play vital roles in
                     extracellular matrix formation, by cleaving precursor
                     proteins such as enzymes, structural proteins; cd04281"
                     /db_xref="CDD:239808"
     Site            order(435..436,439,445)
                     /site_type="active"
                     /db_xref="CDD:239808"
     Region          544..658
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(552,554,556,585,590,629,655,657,659..660)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          662..772
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(671,673,675,702,707,745,769,771)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          777..815
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Region          818..932
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(826,828,830,857,862,905,929,931)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          939..974
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          979..1088
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(989,991,1018,1023,1061,1085,1087,1089..1090)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          1092..1207
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(1101,1103,1107,1135,1140,1178,1202,1204,1206..1207)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     CDS             1..1253
                     /gene="tok"
                     /coded_by="XM_034335117.2:1..3762"
                     /db_xref="GeneID:117609150"
ORIGIN      
        1 mrllflvsif wlacrcadsr avhahstene yrrhrrrftv dqllntkfph gisgdldvdv
       61 ckaggflgdi alpnikyete wrqqklnksy leelekyrde vlkeglqvee eglteilqfk
      121 nehdanephq eneeivasqe ksepimvdrn qysmdgeleg gfsfkarndd arpgvrdegv
      181 efrlesttpa tkkrhsarkq nhdvsqtasn vnatkskqsf lrtsheldnf ststdstnqm
      241 kdanmkniee vftvqpenst qnvskrrhrr hhrrrmskrr hrrrkiplvr rdfddssdev
      301 vslhdrqlrs iefydmehkp kylankrgtt rhgyssrrrr aatarkervw ddgvipyeid
      361 gnfsgahkal fkqamrhwen ftcvkfverv ptehpnyilf terpcgccsf vgkrgngaqa
      421 isigkncdkf givvhelghv vgfwhehtrp drdrhvqiir dnimsgqeyn fnklteeevn
      481 slglpydyds imhyakntfs rgtyldtilp meshgkkrpe igqrlrlseg diaqtnllyk
      541 cykcgrtfqe nsgsfgspih pngspstege rcewritath gerivlnits lnilksnlcr
      601 sdyleirdgy whkskvlgry cgngkiqdpi vstgsrmlvt yvtsghqssg qrgftasyea
      661 vcggeieldd tghlespnyp dgyqsskecv wklsvpqnfq valkfqsfei enhdncvydy
      721 vevrdghnad spligvycgy kippdikstg nkllvkfvsd gsvqkagfsa tfmkefdecv
      781 liehgcehnc intlggfecs cnpgyelhsd gkhcedacgg tfvdsngtit spsfpvtypg
      841 nkdcvweiia ppqyrimlnf thfdlegnna rqqeceydsv evssklgddi lrkhgifcgs
      901 rvpslitseg nfmkitftsd nsiqktgfaa vfltdmdeca nnnggcqhec rntigsyqcs
      961 chngftlhen ghdcreggck yeivapmgti tspnypdyyp glkdcvwhfv tkpghriklv
     1021 fkvfemeshq ecnydhiaiy dgdspdshvl gkfcgtkeph pilatgnqmy mvfksdasiq
     1081 rkgflathst acgghlvats evkhlyshak ygthnydhrt dcdwaieapp gknvhltfvt
     1141 fqlesesecn ydfvevysgl dtsgllygry cgnsnttdfi sineallvrf rtddtisnkg
     1201 fmaffvavdr qdsgenyggs ededadvsgl anrlgsefhi gaalqssdrs ene
//
LOCUS       XP_034191009            1108 aa            linear   INV 03-SEP-2025
DEFINITION  tolloid-like protein 1 tolkin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191009
VERSION     XP_034191009.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335118.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191009.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1108
                     /product="tolloid-like protein 1 tolkin isoform X2"
                     /calculated_mol_wt=124971
     Region          201..396
                     /region_name="ZnMc_BMP1_TLD"
                     /note="Zinc-dependent metalloprotease; BMP1/TLD-like
                     subfamily. BMP1 (Bone morphogenetic protein 1) and TLD
                     (tolloid)-like metalloproteases play vital roles in
                     extracellular matrix formation, by cleaving precursor
                     proteins such as enzymes, structural proteins; cd04281"
                     /db_xref="CDD:239808"
     Site            order(290..291,294,300)
                     /site_type="active"
                     /db_xref="CDD:239808"
     Region          399..513
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(407,409,411,440,445,484,510,512,514..515)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          517..627
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(526,528,530,557,562,600,624,626)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          632..670
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain; smart00179"
                     /db_xref="CDD:214542"
     Region          673..787
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(681,683,685,712,717,760,784,786)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          794..829
                     /region_name="FXa_inhibition"
                     /note="Coagulation Factor Xa inhibitory site; pfam14670"
                     /db_xref="CDD:464251"
     Region          834..943
                     /region_name="CUB"
                     /note="CUB domain; pfam00431"
                     /db_xref="CDD:395345"
     Site            order(844,846,873,878,916,940,942,944..945)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     Region          947..1062
                     /region_name="CUB"
                     /note="CUB domain; extracellular domain; present in
                     proteins mostly known to be involved in development; not
                     found in prokaryotes, plants and yeast; cd00041"
                     /db_xref="CDD:238001"
     Site            order(956,958,962,990,995,1033,1057,1059,1061..1062)
                     /site_type="other"
                     /note="heterodimerization interface [polypeptide binding]"
                     /db_xref="CDD:238001"
     CDS             1..1108
                     /gene="tok"
                     /coded_by="XM_034335118.2:3056..6382"
                     /db_xref="GeneID:117609150"
ORIGIN      
        1 mvdrnqysmd geleggfsfk arnddarpgv rdegvefrle sttpatkkrh sarkqnhdvs
       61 qtasnvnatk skqsflrtsh eldnfststd stnqmkdanm knieevftvq penstqnvsk
      121 rrhrrhhrrr mskrrhrrrk iplvrrdfdd ssdevvslhd rqlrsiefyd mehkpkylan
      181 krgttrhgys srrrraatar kervwddgvi pyeidgnfsg ahkalfkqam rhwenftcvk
      241 fvervptehp nyilfterpc gccsfvgkrg ngaqaisigk ncdkfgivvh elghvvgfwh
      301 ehtrpdrdrh vqiirdnims gqeynfnklt eeevnslglp ydydsimhya kntfsrgtyl
      361 dtilpmeshg kkrpeigqrl rlsegdiaqt nllykcykcg rtfqensgsf gspihpngsp
      421 stegercewr itathgeriv lnitslnilk snlcrsdyle irdgywhksk vlgrycgngk
      481 iqdpivstgs rmlvtyvtsg hqssgqrgft asyeavcgge ielddtghle spnypdgyqs
      541 skecvwklsv pqnfqvalkf qsfeienhdn cvydyvevrd ghnadsplig vycgykippd
      601 ikstgnkllv kfvsdgsvqk agfsatfmke fdecvliehg cehncintlg gfecscnpgy
      661 elhsdgkhce dacggtfvds ngtitspsfp vtypgnkdcv weiiappqyr imlnfthfdl
      721 egnnarqqec eydsvevssk lgddilrkhg ifcgsrvpsl itsegnfmki tftsdnsiqk
      781 tgfaavfltd mdecannngg cqhecrntig syqcschngf tlhenghdcr eggckyeiva
      841 pmgtitspny pdyypglkdc vwhfvtkpgh riklvfkvfe meshqecnyd hiaiydgdsp
      901 dshvlgkfcg tkephpilat gnqmymvfks dasiqrkgfl athstacggh lvatsevkhl
      961 yshakygthn ydhrtdcdwa ieappgknvh ltfvtfqles esecnydfve vysgldtsgl
     1021 lygrycgnsn ttdfisinea llvrfrtddt isnkgfmaff vavdrqdsge nyggsededa
     1081 dvsglanrlg sefhigaalq ssdrsene
//
LOCUS       XP_076548070             998 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548070
VERSION     XP_076548070.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691955.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..998
                     /product="protein trachealess isoform X1"
                     /calculated_mol_wt=107699
     Region          144..220
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(149,151..153,157,159..160,164,185..186)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(162..163,166..167,169..170,187,190,194,196..197,201,
                     203..204)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          245..302
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          440..527
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(443,447,453,466..469,495,500)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(463,467,475,478..479,507,509)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..998
                     /gene="LOC117607156"
                     /coded_by="XM_076691955.1:134..3130"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mhrgggvags gaaaaagiag gsagsgalps tshrgleleh psampgapgf hwgamlagph
       61 aaaaasmmqp plsaggeytp apahhgtthp ampmdlhvhq gfsyyryred vlcwtdrkps
      121 mddvgnptsv narydeehyt fesiqelrke ksrdaarcrr gkenfefyel akmlplpsai
      181 tsqldkasii rltisylklr efsghgdppw nrdgpppnks gkganrvrss aisavdyfdv
      241 dhgthllqsl dgfaiavavd grflyisetv svylglsqve mtgssvfdyv hqqdhaevae
      301 qlglgltsss sgphssssgl aspssaasee hgssstanpd vssvmslstt glykgydraf
      361 cvrmksaiak rgchfkssgf rvvlilcrlr pqytfshtrk sappligmvg laialpppnv
      421 heirldsdmf vtrinfdfri ahceprvsel ldytadeltg knlyalchge danrlrkshi
      481 dlinkgqvlt hyyrlmnksg gytwlqtcat vvcnsknaee qniicvnyvi sgreyenlim
      541 dccqleegvm gvkreeaagn dpengspdad ggegrnhggp pnqhqehphr sppenreesr
      601 gsesekrgsr hhdniaqlqq epqtavgnin tlvvklrghk rklheeesss neeedeedat
      661 vkvtnnersh alspapsnhs isggeqvlcs aspksrrvde rtsnadstgt svkdleqams
      721 khlpvanlnk shsptlhqpt dfstdallkq qqqrstiqwi gahhhlghls pqqsataplp
      781 atallrqlya nresviranv hgitssgstr tpssggtyya gdttpsgplp tppgsegsst
      841 ygehqfvlat hnqkgstgts cadaftslvs systasgytv dyhsamtpps svsprdkqqq
      901 qqqqqlhpvn aissyesssa yadpvlrhqy epaqplplkp qvysaavhqs aldaaaayas
      961 aglteqaqfy hhpaagagfh iyhptnkstt ngwygsts
//
LOCUS       XP_076548071             988 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548071
VERSION     XP_076548071.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691956.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..988
                     /product="protein trachealess isoform X2"
                     /calculated_mol_wt=106397
     Region          134..210
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(139,141..143,147,149..150,154,175..176)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(152..153,156..157,159..160,177,180,184,186..187,191,
                     193..194)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          235..292
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          430..517
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(433,437,443,456..459,485,490)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(453,457,465,468..469,497,499)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..988
                     /gene="LOC117607156"
                     /coded_by="XM_076691956.1:134..3100"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mhrgggvags gaaaaagiag gsagsgalps tshrgleleh psampgapgf hwgamlagph
       61 aaaaasmmqp plsaggeytp apahhgtthp ampmdlhvhq gfsyyryred vlcwtdrkps
      121 mddvgnptsv nariqelrke ksrdaarcrr gkenfefyel akmlplpsai tsqldkasii
      181 rltisylklr efsghgdppw nrdgpppnks gkganrvrss aisavdyfdv dhgthllqsl
      241 dgfaiavavd grflyisetv svylglsqve mtgssvfdyv hqqdhaevae qlglgltsss
      301 sgphssssgl aspssaasee hgssstanpd vssvmslstt glykgydraf cvrmksaiak
      361 rgchfkssgf rvvlilcrlr pqytfshtrk sappligmvg laialpppnv heirldsdmf
      421 vtrinfdfri ahceprvsel ldytadeltg knlyalchge danrlrkshi dlinkgqvlt
      481 hyyrlmnksg gytwlqtcat vvcnsknaee qniicvnyvi sgreyenlim dccqleegvm
      541 gvkreeaagn dpengspdad ggegrnhggp pnqhqehphr sppenreesr gsesekrgsr
      601 hhdniaqlqq epqtavgnin tlvvklrghk rklheeesss neeedeedat vkvtnnersh
      661 alspapsnhs isggeqvlcs aspksrrvde rtsnadstgt svkdleqams khlpvanlnk
      721 shsptlhqpt dfstdallkq qqqrstiqwi gahhhlghls pqqsataplp atallrqlya
      781 nresviranv hgitssgstr tpssggtyya gdttpsgplp tppgsegsst ygehqfvlat
      841 hnqkgstgts cadaftslvs systasgytv dyhsamtpps svsprdkqqq qqqqqlhpvn
      901 aissyesssa yadpvlrhqy epaqplplkp qvysaavhqs aldaaaayas aglteqaqfy
      961 hhpaagagfh iyhptnkstt ngwygsts
//
LOCUS       XP_076548072             982 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076548072
VERSION     XP_076548072.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691957.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..982
                     /product="protein trachealess isoform X3"
                     /calculated_mol_wt=106029
     Region          128..204
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(133,135..137,141,143..144,148,169..170)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(146..147,150..151,153..154,171,174,178,180..181,185,
                     187..188)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          229..286
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          424..511
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(427,431,437,450..453,479,484)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(447,451,459,462..463,491,493)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..982
                     /gene="LOC117607156"
                     /coded_by="XM_076691957.1:134..3082"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mhrgggvags gaaaaagiag gsagsgalps tshrgleleh psampgapgf hwgamlagph
       61 aaaaasmmqp plsaggeytp apahhgtthp ampmdlhvhq gfsyyryred vlcwtdryde
      121 ehytfesiqe lrkeksrdaa rcrrgkenfe fyelakmlpl psaitsqldk asiirltisy
      181 lklrefsghg dppwnrdgpp pnksgkganr vrssaisavd yfdvdhgthl lqsldgfaia
      241 vavdgrflyi setvsvylgl sqvemtgssv fdyvhqqdha evaeqlglgl tssssgphss
      301 ssglaspssa aseehgssst anpdvssvms lsttglykgy drafcvrmks aiakrgchfk
      361 ssgfrvvlil crlrpqytfs htrksappli gmvglaialp ppnvheirld sdmfvtrinf
      421 dfriahcepr vselldytad eltgknlyal chgedanrlr kshidlinkg qvlthyyrlm
      481 nksggytwlq tcatvvcnsk naeeqniicv nyvisgreye nlimdccqle egvmgvkree
      541 aagndpengs pdadggegrn hggppnqhqe hphrsppenr eesrgsesek rgsrhhdnia
      601 qlqqepqtav gnintlvvkl rghkrklhee esssneeede edatvkvtnn ershalspap
      661 snhsisggeq vlcsaspksr rvdertsnad stgtsvkdle qamskhlpva nlnkshsptl
      721 hqptdfstda llkqqqqrst iqwigahhhl ghlspqqsat aplpatallr qlyanresvi
      781 ranvhgitss gstrtpssgg tyyagdttps gplptppgse gsstygehqf vlathnqkgs
      841 tgtscadaft slvssystas gytvdyhsam tppssvsprd kqqqqqqqql hpvnaissye
      901 sssayadpvl rhqyepaqpl plkpqvysaa vhqsaldaaa ayasaglteq aqfyhhpaag
      961 agfhiyhptn ksttngwygs ts
//
LOCUS       XP_076548074             972 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076548074
VERSION     XP_076548074.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691959.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..972
                     /product="protein trachealess isoform X4"
                     /calculated_mol_wt=104658
     Region          118..194
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(123,125..127,131,133..134,138,159..160)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(136..137,140..141,143..144,161,164,168,170..171,175,
                     177..178)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          219..276
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          414..501
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(417,421,427,440..443,469,474)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(437,441,449,452..453,481,483)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..972
                     /gene="LOC117607156"
                     /coded_by="XM_076691959.1:134..3052"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mhrgggvags gaaaaagiag gsagsgalps tshrgleleh psampgapgf hwgamlagph
       61 aaaaasmmqp plsaggeytp apahhgtthp ampmdlhvhq gfsyyryred vlcwtdsiqe
      121 lrkeksrdaa rcrrgkenfe fyelakmlpl psaitsqldk asiirltisy lklrefsghg
      181 dppwnrdgpp pnksgkganr vrssaisavd yfdvdhgthl lqsldgfaia vavdgrflyi
      241 setvsvylgl sqvemtgssv fdyvhqqdha evaeqlglgl tssssgphss ssglaspssa
      301 aseehgssst anpdvssvms lsttglykgy drafcvrmks aiakrgchfk ssgfrvvlil
      361 crlrpqytfs htrksappli gmvglaialp ppnvheirld sdmfvtrinf dfriahcepr
      421 vselldytad eltgknlyal chgedanrlr kshidlinkg qvlthyyrlm nksggytwlq
      481 tcatvvcnsk naeeqniicv nyvisgreye nlimdccqle egvmgvkree aagndpengs
      541 pdadggegrn hggppnqhqe hphrsppenr eesrgsesek rgsrhhdnia qlqqepqtav
      601 gnintlvvkl rghkrklhee esssneeede edatvkvtnn ershalspap snhsisggeq
      661 vlcsaspksr rvdertsnad stgtsvkdle qamskhlpva nlnkshsptl hqptdfstda
      721 llkqqqqrst iqwigahhhl ghlspqqsat aplpatallr qlyanresvi ranvhgitss
      781 gstrtpssgg tyyagdttps gplptppgse gsstygehqf vlathnqkgs tgtscadaft
      841 slvssystas gytvdyhsam tppssvsprd kqqqqqqqql hpvnaissye sssayadpvl
      901 rhqyepaqpl plkpqvysaa vhqsaldaaa ayasaglteq aqfyhhpaag agfhiyhptn
      961 ksttngwygs ts
//
LOCUS       XP_076548075             932 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_076548075
VERSION     XP_076548075.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691960.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..932
                     /product="protein trachealess isoform X5"
                     /calculated_mol_wt=100759
     Region          78..154
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(83,85..87,91,93..94,98,119..120)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(96..97,100..101,103..104,121,124,128,130..131,135,
                     137..138)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          179..236
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          374..461
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(377,381,387,400..403,429,434)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(397,401,409,412..413,441,443)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..932
                     /gene="LOC117607156"
                     /coded_by="XM_076691960.1:540..3338"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mlpyqmpmdy ggfqrqssvg vgvgvgvgvg vgvdvggphq gaagalninp afthswfvpa
       61 dlcvpykqsq gpllepgiqe lrkeksrdaa rcrrgkenfe fyelakmlpl psaitsqldk
      121 asiirltisy lklrefsghg dppwnrdgpp pnksgkganr vrssaisavd yfdvdhgthl
      181 lqsldgfaia vavdgrflyi setvsvylgl sqvemtgssv fdyvhqqdha evaeqlglgl
      241 tssssgphss ssglaspssa aseehgssst anpdvssvms lsttglykgy drafcvrmks
      301 aiakrgchfk ssgfrvvlil crlrpqytfs htrksappli gmvglaialp ppnvheirld
      361 sdmfvtrinf dfriahcepr vselldytad eltgknlyal chgedanrlr kshidlinkg
      421 qvlthyyrlm nksggytwlq tcatvvcnsk naeeqniicv nyvisgreye nlimdccqle
      481 egvmgvkree aagndpengs pdadggegrn hggppnqhqe hphrsppenr eesrgsesek
      541 rgsrhhdnia qlqqepqtav gnintlvvkl rghkrklhee esssneeede edatvkvtnn
      601 ershalspap snhsisggeq vlcsaspksr rvdertsnad stgtsvkdle qamskhlpva
      661 nlnkshsptl hqptdfstda llkqqqqrst iqwigahhhl ghlspqqsat aplpatallr
      721 qlyanresvi ranvhgitss gstrtpssgg tyyagdttps gplptppgse gsstygehqf
      781 vlathnqkgs tgtscadaft slvssystas gytvdyhsam tppssvsprd kqqqqqqqql
      841 hpvnaissye sssayadpvl rhqyepaqpl plkpqvysaa vhqsaldaaa ayasaglteq
      901 aqfyhhpaag agfhiyhptn ksttngwygs ts
//
LOCUS       XP_076548076             911 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_076548076
VERSION     XP_076548076.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691961.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..911
                     /product="protein trachealess isoform X6"
                     /calculated_mol_wt=98435
     Region          57..133
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(62,64..66,70,72..73,77,98..99)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(75..76,79..80,82..83,100,103,107,109..110,114,
                     116..117)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          158..215
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          353..440
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(356,360,366,379..382,408,413)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(376,380,388,391..392,420,422)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..911
                     /gene="LOC117607156"
                     /coded_by="XM_076691961.1:540..3275"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mlpyqmpmdy ggfqrqssvg vgvgvgvgvg vgvdvggphq gaagalninp afthsciqel
       61 rkeksrdaar crrgkenfef yelakmlplp saitsqldka siirltisyl klrefsghgd
      121 ppwnrdgppp nksgkganrv rssaisavdy fdvdhgthll qsldgfaiav avdgrflyis
      181 etvsvylgls qvemtgssvf dyvhqqdhae vaeqlglglt ssssgphsss sglaspssaa
      241 seehgsssta npdvssvmsl sttglykgyd rafcvrmksa iakrgchfks sgfrvvlilc
      301 rlrpqytfsh trksapplig mvglaialpp pnvheirlds dmfvtrinfd friahceprv
      361 selldytade ltgknlyalc hgedanrlrk shidlinkgq vlthyyrlmn ksggytwlqt
      421 catvvcnskn aeeqniicvn yvisgreyen limdccqlee gvmgvkreea agndpengsp
      481 dadggegrnh ggppnqhqeh phrsppenre esrgsesekr gsrhhdniaq lqqepqtavg
      541 nintlvvklr ghkrklheee sssneeedee datvkvtnne rshalspaps nhsisggeqv
      601 lcsaspksrr vdertsnads tgtsvkdleq amskhlpvan lnkshsptlh qptdfstdal
      661 lkqqqqrsti qwigahhhlg hlspqqsata plpatallrq lyanresvir anvhgitssg
      721 strtpssggt yyagdttpsg plptppgseg sstygehqfv lathnqkgst gtscadafts
      781 lvssystasg ytvdyhsamt ppssvsprdk qqqqqqqqlh pvnaissyes ssayadpvlr
      841 hqyepaqplp lkpqvysaav hqsaldaaaa yasaglteqa qfyhhpaaga gfhiyhptnk
      901 sttngwygst s
//
LOCUS       XP_076548077             864 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076548077
VERSION     XP_076548077.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691962.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..864
                     /product="protein trachealess isoform X7"
                     /calculated_mol_wt=93910
     Region          10..86
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(15,17..19,23,25..26,30,51..52)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(28..29,32..33,35..36,53,56,60,62..63,67,69..70)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          111..168
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          306..393
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(309,313,319,332..335,361,366)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(329,333,341,344..345,373,375)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..864
                     /gene="LOC117607156"
                     /coded_by="XM_076691962.1:20..2614"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mihvhlpaci qelrkeksrd aarcrrgken fefyelakml plpsaitsql dkasiirlti
       61 sylklrefsg hgdppwnrdg pppnksgkga nrvrssaisa vdyfdvdhgt hllqsldgfa
      121 iavavdgrfl yisetvsvyl glsqvemtgs svfdyvhqqd haevaeqlgl gltssssgph
      181 ssssglasps saaseehgss stanpdvssv mslsttglyk gydrafcvrm ksaiakrgch
      241 fkssgfrvvl ilcrlrpqyt fshtrksapp ligmvglaia lpppnvheir ldsdmfvtri
      301 nfdfriahce prvselldyt adeltgknly alchgedanr lrkshidlin kgqvlthyyr
      361 lmnksggytw lqtcatvvcn sknaeeqnii cvnyvisgre yenlimdccq leegvmgvkr
      421 eeaagndpen gspdadggeg rnhggppnqh qehphrsppe nreesrgses ekrgsrhhdn
      481 iaqlqqepqt avgnintlvv klrghkrklh eeesssneee deedatvkvt nnershalsp
      541 apsnhsisgg eqvlcsaspk srrvdertsn adstgtsvkd leqamskhlp vanlnkshsp
      601 tlhqptdfst dallkqqqqr stiqwigahh hlghlspqqs ataplpatal lrqlyanres
      661 viranvhgit ssgstrtpss ggtyyagdtt psgplptppg segsstygeh qfvlathnqk
      721 gstgtscada ftslvssyst asgytvdyhs amtppssvsp rdkqqqqqqq qlhpvnaiss
      781 yesssayadp vlrhqyepaq plplkpqvys aavhqsalda aaayasaglt eqaqfyhhpa
      841 agagfhiyhp tnksttngwy gsts
//
LOCUS       XP_076548078             689 aa            linear   INV 03-SEP-2025
DEFINITION  protein trachealess isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_076548078
VERSION     XP_076548078.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691963.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..689
                     /product="protein trachealess isoform X8"
                     /calculated_mol_wt=75003
     Region          144..220
                     /region_name="bHLH-PAS_trachealess_like"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in Drosophila melanogaster protein
                     trachealess and similar proteins; cd19733"
                     /db_xref="CDD:381576"
     Site            order(149,151..153,157,159..160,164,185..186)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381576"
     Site            order(162..163,166..167,169..170,187,190,194,196..197,201,
                     203..204)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381576"
     Region          245..302
                     /region_name="PAS"
                     /note="PAS domain; smart00091"
                     /db_xref="CDD:214512"
     Region          440..527
                     /region_name="PAS_3"
                     /note="PAS fold; pfam08447"
                     /db_xref="CDD:430001"
     Site            order(443,447,453,466..469,495,500)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238075"
     Site            order(463,467,475,478..479,507,509)
                     /site_type="other"
                     /note="heme pocket [chemical binding]"
                     /db_xref="CDD:238075"
     CDS             1..689
                     /gene="LOC117607156"
                     /coded_by="XM_076691963.1:134..2203"
                     /db_xref="GeneID:117607156"
ORIGIN      
        1 mhrgggvags gaaaaagiag gsagsgalps tshrgleleh psampgapgf hwgamlagph
       61 aaaaasmmqp plsaggeytp apahhgtthp ampmdlhvhq gfsyyryred vlcwtdrkps
      121 mddvgnptsv narydeehyt fesiqelrke ksrdaarcrr gkenfefyel akmlplpsai
      181 tsqldkasii rltisylklr efsghgdppw nrdgpppnks gkganrvrss aisavdyfdv
      241 dhgthllqsl dgfaiavavd grflyisetv svylglsqve mtgssvfdyv hqqdhaevae
      301 qlglgltsss sgphssssgl aspssaasee hgssstanpd vssvmslstt glykgydraf
      361 cvrmksaiak rgchfkssgf rvvlilcrlr pqytfshtrk sappligmvg laialpppnv
      421 heirldsdmf vtrinfdfri ahceprvsel ldytadeltg knlyalchge danrlrkshi
      481 dlinkgqvlt hyyrlmnksg gytwlqtcat vvcnsknaee qniicvnyvi sgreyenlim
      541 dccqleegvm gvkreeaagn dpengspdad ggegrnhggp pnqhqehphr sppenreesr
      601 gsesekrgsr hhdniaqlqq epqtavgnin tlvvklrghk rklheeesss neeedeedat
      661 evskycvrpv qnpdawmner atptvpapp
//
LOCUS       XP_034186734             793 aa            linear   INV 03-SEP-2025
DEFINITION  conserved oligomeric Golgi complex subunit 3 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186734
VERSION     XP_034186734.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330843.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..793
                     /product="conserved oligomeric Golgi complex subunit 3
                     isoform X1"
                     /calculated_mol_wt=91868
     Region          86..230
                     /region_name="Sec34"
                     /note="Sec34-like family; pfam04136"
                     /db_xref="CDD:461190"
     CDS             1..793
                     /gene="Cog3"
                     /coded_by="XM_034330843.2:474..2855"
                     /db_xref="GeneID:117607299"
ORIGIN      
        1 msnlkdipnn ltkwdlseds lapltdlqre cllnledels sineakiely eekkpaqieq
       61 yqdlleyyas lekkcmdrkd mkyilylkel earrlechel clqieealed ftmlykqyse
      121 vsekttslyd aseqldsdqr rlnsiienit eyvkyfkdvd mmmekleapt lcvnsemffn
      181 ildkidknid flqnnpsfke sntylmkykh cqskaismmq nyifnlfskt tesilnlkdn
      241 dgsqdnadaa lalfygrfqt ilskvksvie qiemksykrq eydsllsech qyywsqrglv
      301 lgssvqkslm svkekyngdh cslvrnscal llhasmdeyk lfyeffskps nglttylesl
      361 ctslydtlrp fiihinhlet laeiccilri emldehvqnn leplegfgni clqllhdvqe
      421 rlvfrahlyl qsdvlnysps pgdlaypekl kmmediaesl reesrqtrmk kisissnsdn
      481 iaesvsrnhi eidsiyqkth mgnspadlhg mwyptvrrtl vclsrlyrcv drsvfqslsq
      541 eaislcvqsi enarqeiekr aslldaelfq ikhllilreq iapfqvdfti keysldfskv
      601 ktaafgllek ssrfftlsnn sllefllegt pqmkeqlids rkhvdnklky acqrliqhat
      661 fslihpilkl qekeksyvna snqnisqeha lgsaqdvaaa isevlriikf kcpeiqqlmq
      721 lylsnketef ilfrpvknnv caaftqlyql lskyynseel qliacplpeq isvmlssssl
      781 vhgkstqttv kkt
//
LOCUS       XP_034186735             772 aa            linear   INV 03-SEP-2025
DEFINITION  conserved oligomeric Golgi complex subunit 3 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186735
VERSION     XP_034186735.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330844.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..772
                     /product="conserved oligomeric Golgi complex subunit 3
                     isoform X2"
                     /calculated_mol_wt=89342
     Region          86..209
                     /region_name="Sec34"
                     /note="Sec34-like family; pfam04136"
                     /db_xref="CDD:461190"
     CDS             1..772
                     /gene="Cog3"
                     /coded_by="XM_034330844.2:551..2869"
                     /db_xref="GeneID:117607299"
ORIGIN      
        1 msnlkdipnn ltkwdlseds lapltdlqre cllnledels sineakiely eekkpaqieq
       61 yqdlleyyas lekkcmdrkd mkyilylkel earrlechel clqieealed ftmlykqyse
      121 vsekttslyd aseqldsdqr rlnsiienit eyvkyfkdvd mmmekleapt lcnnpsfkes
      181 ntylmkykhc qskaismmqn yifnlfsktt esilnlkdnd gsqdnadaal alfygrfqti
      241 lskvksvieq iemksykrqe ydsllsechq yywsqrglvl gssvqkslms vkekyngdhc
      301 slvrnscall lhasmdeykl fyeffskpsn glttyleslc tslydtlrpf iihinhletl
      361 aeiccilrie mldehvqnnl eplegfgnic lqllhdvqer lvfrahlylq sdvlnyspsp
      421 gdlaypeklk mmediaeslr eesrqtrmkk isissnsdni aesvsrnhie idsiyqkthm
      481 gnspadlhgm wyptvrrtlv clsrlyrcvd rsvfqslsqe aislcvqsie narqeiekra
      541 slldaelfqi khllilreqi apfqvdftik eysldfskvk taafglleks srfftlsnns
      601 llefllegtp qmkeqlidsr khvdnklkya cqrliqhatf slihpilklq ekeksyvnas
      661 nqnisqehal gsaqdvaaai sevlriikfk cpeiqqlmql ylsnketefi lfrpvknnvc
      721 aaftqlyqll skyynseelq liacplpeqi svmlsssslv hgkstqttvk kt
//
LOCUS       XP_034191010             652 aa            linear   INV 03-SEP-2025
DEFINITION  sodium- and chloride-dependent GABA transporter 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191010
VERSION     XP_034191010.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335119.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191010.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..652
                     /product="sodium- and chloride-dependent GABA transporter
                     1"
                     /calculated_mol_wt=72771
     Region          66..616
                     /region_name="SLC6sbd-TauT-like"
                     /note="Na(+)- and Cl(-)-dependent taurine transporter
                     TauT, and related proteins; solute-binding domain;
                     cd11496"
                     /db_xref="CDD:271387"
     Site            order(81,84,434,437..438)
                     /site_type="other"
                     /note="Na binding site 2 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(82..83,85..88,163,336..337,342,344,438,441..442)
                     /site_type="other"
                     /note="putative substrate binding site 1 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     Site            order(83,88,337,369)
                     /site_type="other"
                     /note="Na binding site 1 [ion binding]"
                     /db_xref="CDD:271387"
     Site            order(90..91,162,166,169,403,407)
                     /site_type="other"
                     /note="putative substrate binding site 2 [chemical
                     binding]"
                     /db_xref="CDD:271387"
     CDS             1..652
                     /gene="LOC117609151"
                     /coded_by="XM_034335119.2:504..2462"
                     /db_xref="GeneID:117609151"
ORIGIN      
        1 maekmyywge ekdqvdpdqa fiefkakpva aerkrdytsa svpkmtassp qgratevgnr
       61 eedterggwd nkldflfsci svsvglgnvw rfpylcykng ggvflitygi amlfcgipif
      121 fqevaigqyl gaggmtlvgq lcpllqgvgy atmtivffld vyyciiiawt lfylistfan
      181 ipgvpwsgcg nwwntkdcya gsdkiehggn vsysnntnvt snvtlhhttp veeywerrvl
      241 gitsgietig giqyellgcl vigwllvysi irrglhqsgk iiwfsalfpy vvlfillgra
      301 vtldgaydgl lyyvtprwdq llspgpwidg atqiffaysi gtgalpalgs ynkfhhnsyk
      361 dalitcivnt ltcllagcvt fsilghiale qntqvsevvk sgpglvflty pevvlklpga
      421 piwaiiffvm llilgidsef civesfitgm vdnwpdllrp hrkkftiaic llmfllgipm
      481 vthggvyifq lmdfysasgm silwvcffqt iaiswifgan kfcdcihqmm gvrlnkfwyi
      541 cwvvfapvim afifvfqcvq ykplkygsdy eyptwaevlg fclslssmiw ipvyaiyyvl
      601 vtpgsikeni lkglkpniks hpklpkgeks avipmsessa glitknnsfl rg
//
LOCUS       XP_034191012             651 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609152 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191012
VERSION     XP_034191012.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335121.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191012.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..651
                     /product="uncharacterized protein LOC117609152 isoform X1"
                     /calculated_mol_wt=71728
     Region          96..604
                     /region_name="ascorbase"
                     /note="L-ascorbate oxidase, plant type; TIGR03388"
                     /db_xref="CDD:274555"
     Region          96..200
                     /region_name="CuRO_1_tcLCC2_insect_like"
                     /note="The first cupredoxin domain of insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13858"
                     /db_xref="CDD:259927"
     Site            order(101,105..106,110,115,134,140,175..180,182,189..191,
                     194..196,198)
                     /site_type="other"
                     /note="putative Domain 2 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Site            order(138,140,143,167..168,182,184,186,189..190)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Region          218..357
                     /region_name="CuRO_2_tcLCC_insect_like"
                     /note="The second cupredoxin domain of the insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13884"
                     /db_xref="CDD:259951"
     Site            order(219..222,226,243,246..249,262,272,274,276,301..303,
                     318,320,351,353..354)
                     /site_type="other"
                     /note="putative Domain 1 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     Site            order(283,285,297..298,300..304,306..308,310,314,316..321)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     CDS             1..651
                     /gene="LOC117609152"
                     /coded_by="XM_034335121.2:853..2808"
                     /db_xref="GeneID:117609152"
ORIGIN      
        1 mrslksthyt mtlstyclrf lvvvfmiaaa atflhvnnyr pkqiflscdr pchhldwpmi
       61 crlkltlevf qslskscgdc pvnetaclhn hcvtadgqrr giltanrqlp gptiqvcend
      121 ilvvdvinrl pgkaaamhwr gqsqfespfm dgaplvtqcp ipsyttfqyk frasvagthl
      181 whahagadvt ngifgalivk qadikdphrs lydiddsnhv vlvsqwqhsa ditfteghak
      241 paillingkg rqpngpnvpy stftvvpgrr hrfrianagg agscpitisv dahplqlial
      301 dgqpveprlv asitlakger adfilkankr vasywmnvht skecgtspih gaailkykgs
      361 ttenplsvse aidqpeveea tsrvamttnp aekcenpenl cvteiqalrk ipsvlakprt
      421 dviirlpiny kfqtndivgn ggaetrilnv nndtftypss plltqgadvs eeslcspddq
      481 dsrnenneta slssrrcrrg dgivpdtcec vhvryvplga tveiilfdqg glddlvyhmh
      541 gysfyvvgak kfgrsvslhe lksldekgql fnrnldctvs kdtvvvpkfg avairfkann
      601 pgywmlrdeh aaywtrgldv ilqvgensdm vpapqdfpkc gsfvgpdyfl i
//
LOCUS       XP_034191013             651 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609152 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191013
VERSION     XP_034191013.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191013.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..651
                     /product="uncharacterized protein LOC117609152 isoform X1"
                     /calculated_mol_wt=71728
     Region          96..604
                     /region_name="ascorbase"
                     /note="L-ascorbate oxidase, plant type; TIGR03388"
                     /db_xref="CDD:274555"
     Region          96..200
                     /region_name="CuRO_1_tcLCC2_insect_like"
                     /note="The first cupredoxin domain of insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13858"
                     /db_xref="CDD:259927"
     Site            order(101,105..106,110,115,134,140,175..180,182,189..191,
                     194..196,198)
                     /site_type="other"
                     /note="putative Domain 2 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Site            order(138,140,143,167..168,182,184,186,189..190)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Region          218..357
                     /region_name="CuRO_2_tcLCC_insect_like"
                     /note="The second cupredoxin domain of the insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13884"
                     /db_xref="CDD:259951"
     Site            order(219..222,226,243,246..249,262,272,274,276,301..303,
                     318,320,351,353..354)
                     /site_type="other"
                     /note="putative Domain 1 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     Site            order(283,285,297..298,300..304,306..308,310,314,316..321)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     CDS             1..651
                     /gene="LOC117609152"
                     /coded_by="XM_034335122.2:1273..3228"
                     /db_xref="GeneID:117609152"
ORIGIN      
        1 mrslksthyt mtlstyclrf lvvvfmiaaa atflhvnnyr pkqiflscdr pchhldwpmi
       61 crlkltlevf qslskscgdc pvnetaclhn hcvtadgqrr giltanrqlp gptiqvcend
      121 ilvvdvinrl pgkaaamhwr gqsqfespfm dgaplvtqcp ipsyttfqyk frasvagthl
      181 whahagadvt ngifgalivk qadikdphrs lydiddsnhv vlvsqwqhsa ditfteghak
      241 paillingkg rqpngpnvpy stftvvpgrr hrfrianagg agscpitisv dahplqlial
      301 dgqpveprlv asitlakger adfilkankr vasywmnvht skecgtspih gaailkykgs
      361 ttenplsvse aidqpeveea tsrvamttnp aekcenpenl cvteiqalrk ipsvlakprt
      421 dviirlpiny kfqtndivgn ggaetrilnv nndtftypss plltqgadvs eeslcspddq
      481 dsrnenneta slssrrcrrg dgivpdtcec vhvryvplga tveiilfdqg glddlvyhmh
      541 gysfyvvgak kfgrsvslhe lksldekgql fnrnldctvs kdtvvvpkfg avairfkann
      601 pgywmlrdeh aaywtrgldv ilqvgensdm vpapqdfpkc gsfvgpdyfl i
//
LOCUS       XP_034191014             635 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609152 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191014
VERSION     XP_034191014.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335123.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191014.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..635
                     /product="uncharacterized protein LOC117609152 isoform X2"
                     /calculated_mol_wt=69778
     Region          80..588
                     /region_name="ascorbase"
                     /note="L-ascorbate oxidase, plant type; TIGR03388"
                     /db_xref="CDD:274555"
     Region          80..184
                     /region_name="CuRO_1_tcLCC2_insect_like"
                     /note="The first cupredoxin domain of insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13858"
                     /db_xref="CDD:259927"
     Site            order(85,89..90,94,99,118,124,159..164,166,173..175,
                     178..180,182)
                     /site_type="other"
                     /note="putative Domain 2 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Site            order(122,124,127,151..152,166,168,170,173..174)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Region          202..341
                     /region_name="CuRO_2_tcLCC_insect_like"
                     /note="The second cupredoxin domain of the insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13884"
                     /db_xref="CDD:259951"
     Site            order(203..206,210,227,230..233,246,256,258,260,285..287,
                     302,304,335,337..338)
                     /site_type="other"
                     /note="putative Domain 1 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     Site            order(267,269,281..282,284..288,290..292,294,298,300..305)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     CDS             1..635
                     /gene="LOC117609152"
                     /coded_by="XM_034335123.2:772..2679"
                     /db_xref="GeneID:117609152"
ORIGIN      
        1 mffrfaifvg ltsvvatiiy ltpipeqifl scdrpchhld wpmicrlklt levfqslsks
       61 cgdcpvneta clhnhcvtad gqrrgiltan rqlpgptiqv cendilvvdv inrlpgkaaa
      121 mhwrgqsqfe spfmdgaplv tqcpipsytt fqykfrasva gthlwhahag advtngifga
      181 livkqadikd phrslydidd snhvvlvsqw qhsaditfte ghakpailli ngkgrqpngp
      241 nvpystftvv pgrrhrfria naggagscpi tisvdahplq lialdgqpve prlvasitla
      301 kgeradfilk ankrvasywm nvhtskecgt spihgaailk ykgsttenpl svseaidqpe
      361 veeatsrvam ttnpaekcen penlcvteiq alrkipsvla kprtdviirl pinykfqtnd
      421 ivgnggaetr ilnvnndtft ypssplltqg advseeslcs pddqdsrnen netaslssrr
      481 crrgdgivpd tcecvhvryv plgatveiil fdqgglddlv yhmhgysfyv vgakkfgrsv
      541 slhelkslde kgqlfnrnld ctvskdtvvv pkfgavairf kannpgywml rdehaaywtr
      601 gldvilqvge nsdmvpapqd fpkcgsfvgp dyfli
//
LOCUS       XP_034191015             593 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609152 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191015
VERSION     XP_034191015.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335124.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191015.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..593
                     /product="uncharacterized protein LOC117609152 isoform X3"
                     /calculated_mol_wt=64900
     Region          38..546
                     /region_name="ascorbase"
                     /note="L-ascorbate oxidase, plant type; TIGR03388"
                     /db_xref="CDD:274555"
     Region          38..142
                     /region_name="CuRO_1_tcLCC2_insect_like"
                     /note="The first cupredoxin domain of insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13858"
                     /db_xref="CDD:259927"
     Site            order(43,47..48,52,57,76,82,117..122,124,131..133,
                     136..138,140)
                     /site_type="other"
                     /note="putative Domain 2 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Site            order(80,82,85,109..110,124,126,128,131..132)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259927"
     Region          160..299
                     /region_name="CuRO_2_tcLCC_insect_like"
                     /note="The second cupredoxin domain of the insect laccases
                     similar to laccase 2 in Tribolium castaneum; cd13884"
                     /db_xref="CDD:259951"
     Site            order(161..164,168,185,188..191,204,214,216,218,243..245,
                     260,262,293,295..296)
                     /site_type="other"
                     /note="putative Domain 1 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     Site            order(225,227,239..240,242..246,248..250,252,256,258..263)
                     /site_type="other"
                     /note="putative Domain 3 interface [polypeptide binding]"
                     /db_xref="CDD:259951"
     CDS             1..593
                     /gene="LOC117609152"
                     /coded_by="XM_034335124.2:641..2422"
                     /db_xref="GeneID:117609152"
ORIGIN      
        1 micrlkltle vfqslskscg dcpvnetacl hnhcvtadgq rrgiltanrq lpgptiqvce
       61 ndilvvdvin rlpgkaaamh wrgqsqfesp fmdgaplvtq cpipsyttfq ykfrasvagt
      121 hlwhahagad vtngifgali vkqadikdph rslydiddsn hvvlvsqwqh saditftegh
      181 akpailling kgrqpngpnv pystftvvpg rrhrfriana ggagscpiti svdahplqli
      241 aldgqpvepr lvasitlakg eradfilkan krvasywmnv htskecgtsp ihgaailkyk
      301 gsttenplsv seaidqpeve eatsrvamtt npaekcenpe nlcvteiqal rkipsvlakp
      361 rtdviirlpi nykfqtndiv gnggaetril nvnndtftyp ssplltqgad vseeslcspd
      421 dqdsrnenne taslssrrcr rgdgivpdtc ecvhvryvpl gatveiilfd qgglddlvyh
      481 mhgysfyvvg akkfgrsvsl helksldekg qlfnrnldct vskdtvvvpk fgavairfka
      541 nnpgywmlrd ehaaywtrgl dvilqvgens dmvpapqdfp kcgsfvgpdy fli
//
LOCUS       XP_076548079             533 aa            linear   INV 03-SEP-2025
DEFINITION  pre-piRNA 3'-exonuclease trimmer isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548079
VERSION     XP_076548079.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691964.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="pre-piRNA 3'-exonuclease trimmer isoform X1"
                     /calculated_mol_wt=61972
     Region          4..373
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; pfam04857"
                     /db_xref="CDD:461457"
     CDS             1..533
                     /gene="LOC117609154"
                     /coded_by="XM_076691964.1:140..1741"
                     /db_xref="GeneID:117609154"
ORIGIN      
        1 mnevldenfe efypelekli nnasfiaidt eltgistdnd vkyslfdsld vrykklknti
       61 enfsiiqfgi svfhhvpdin fydaqcfnfy llpralpltn nqitwqvgal eflskhnfdf
      121 nklvnhaipy lnesdeknfk qyieksssld klkhlsyede tnfreciskt tdwiyndvhq
      181 tsfelevedp ivqylirkrl ndydnirtts gykriiveri sskntcdvse kedsncleqa
      241 lldsyvgfsk vfkllcsskk piighnvlfd lmftyqqfyk plpdsytefk snvhslfpqi
      301 ydtkfistel rrlyikevnw klssldavye fftlkqgkyl tlnsptvnlr lksldekyyh
      361 hagwdayfag yifiklghvf gvkiygsgle erpithselm ssvknyvnsv nvsrsnemcm
      421 kldgddplys kpewlhvklk sasidtkqlv erlssfgqvd impfarkrvl vavsnqksam
      481 rilhhfqssk efqvarynri khaapsticl wsgiilsggl fawiikqtll rst
//
LOCUS       XP_034191016             533 aa            linear   INV 03-SEP-2025
DEFINITION  pre-piRNA 3'-exonuclease trimmer isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191016
VERSION     XP_034191016.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335125.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="pre-piRNA 3'-exonuclease trimmer isoform X1"
                     /calculated_mol_wt=61972
     Region          4..373
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; pfam04857"
                     /db_xref="CDD:461457"
     CDS             1..533
                     /gene="LOC117609154"
                     /coded_by="XM_034335125.2:286..1887"
                     /db_xref="GeneID:117609154"
ORIGIN      
        1 mnevldenfe efypelekli nnasfiaidt eltgistdnd vkyslfdsld vrykklknti
       61 enfsiiqfgi svfhhvpdin fydaqcfnfy llpralpltn nqitwqvgal eflskhnfdf
      121 nklvnhaipy lnesdeknfk qyieksssld klkhlsyede tnfreciskt tdwiyndvhq
      181 tsfelevedp ivqylirkrl ndydnirtts gykriiveri sskntcdvse kedsncleqa
      241 lldsyvgfsk vfkllcsskk piighnvlfd lmftyqqfyk plpdsytefk snvhslfpqi
      301 ydtkfistel rrlyikevnw klssldavye fftlkqgkyl tlnsptvnlr lksldekyyh
      361 hagwdayfag yifiklghvf gvkiygsgle erpithselm ssvknyvnsv nvsrsnemcm
      421 kldgddplys kpewlhvklk sasidtkqlv erlssfgqvd impfarkrvl vavsnqksam
      481 rilhhfqssk efqvarynri khaapsticl wsgiilsggl fawiikqtll rst
//
LOCUS       XP_034191017             533 aa            linear   INV 03-SEP-2025
DEFINITION  pre-piRNA 3'-exonuclease trimmer isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191017
VERSION     XP_034191017.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335126.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="pre-piRNA 3'-exonuclease trimmer isoform X1"
                     /calculated_mol_wt=61972
     Region          4..373
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; pfam04857"
                     /db_xref="CDD:461457"
     CDS             1..533
                     /gene="LOC117609154"
                     /coded_by="XM_034335126.2:143..1744"
                     /db_xref="GeneID:117609154"
ORIGIN      
        1 mnevldenfe efypelekli nnasfiaidt eltgistdnd vkyslfdsld vrykklknti
       61 enfsiiqfgi svfhhvpdin fydaqcfnfy llpralpltn nqitwqvgal eflskhnfdf
      121 nklvnhaipy lnesdeknfk qyieksssld klkhlsyede tnfreciskt tdwiyndvhq
      181 tsfelevedp ivqylirkrl ndydnirtts gykriiveri sskntcdvse kedsncleqa
      241 lldsyvgfsk vfkllcsskk piighnvlfd lmftyqqfyk plpdsytefk snvhslfpqi
      301 ydtkfistel rrlyikevnw klssldavye fftlkqgkyl tlnsptvnlr lksldekyyh
      361 hagwdayfag yifiklghvf gvkiygsgle erpithselm ssvknyvnsv nvsrsnemcm
      421 kldgddplys kpewlhvklk sasidtkqlv erlssfgqvd impfarkrvl vavsnqksam
      481 rilhhfqssk efqvarynri khaapsticl wsgiilsggl fawiikqtll rst
//
LOCUS       XP_034191018             533 aa            linear   INV 03-SEP-2025
DEFINITION  pre-piRNA 3'-exonuclease trimmer isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191018
VERSION     XP_034191018.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335127.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="pre-piRNA 3'-exonuclease trimmer isoform X1"
                     /calculated_mol_wt=61972
     Region          4..373
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; pfam04857"
                     /db_xref="CDD:461457"
     CDS             1..533
                     /gene="LOC117609154"
                     /coded_by="XM_034335127.2:167..1768"
                     /db_xref="GeneID:117609154"
ORIGIN      
        1 mnevldenfe efypelekli nnasfiaidt eltgistdnd vkyslfdsld vrykklknti
       61 enfsiiqfgi svfhhvpdin fydaqcfnfy llpralpltn nqitwqvgal eflskhnfdf
      121 nklvnhaipy lnesdeknfk qyieksssld klkhlsyede tnfreciskt tdwiyndvhq
      181 tsfelevedp ivqylirkrl ndydnirtts gykriiveri sskntcdvse kedsncleqa
      241 lldsyvgfsk vfkllcsskk piighnvlfd lmftyqqfyk plpdsytefk snvhslfpqi
      301 ydtkfistel rrlyikevnw klssldavye fftlkqgkyl tlnsptvnlr lksldekyyh
      361 hagwdayfag yifiklghvf gvkiygsgle erpithselm ssvknyvnsv nvsrsnemcm
      421 kldgddplys kpewlhvklk sasidtkqlv erlssfgqvd impfarkrvl vavsnqksam
      481 rilhhfqssk efqvarynri khaapsticl wsgiilsggl fawiikqtll rst
//
LOCUS       XP_034191021             525 aa            linear   INV 03-SEP-2025
DEFINITION  pre-piRNA 3'-exonuclease trimmer isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191021
VERSION     XP_034191021.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335130.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="pre-piRNA 3'-exonuclease trimmer isoform X2"
                     /calculated_mol_wt=61086
     Region          4..373
                     /region_name="CAF1"
                     /note="CAF1 family ribonuclease; pfam04857"
                     /db_xref="CDD:461457"
     CDS             1..525
                     /gene="LOC117609154"
                     /coded_by="XM_034335130.2:140..1717"
                     /db_xref="GeneID:117609154"
ORIGIN      
        1 mnevldenfe efypelekli nnasfiaidt eltgistdnd vkyslfdsld vrykklknti
       61 enfsiiqfgi svfhhvpdin fydaqcfnfy llpralpltn nqitwqvgal eflskhnfdf
      121 nklvnhaipy lnesdeknfk qyieksssld klkhlsyede tnfreciskt tdwiyndvhq
      181 tsfelevedp ivqylirkrl ndydnirtts gykriiveri sskntcdvse kedsncleqa
      241 lldsyvgfsk vfkllcsskk piighnvlfd lmftyqqfyk plpdsytefk snvhslfpqi
      301 ydtkfistel rrlyikevnw klssldavye fftlkqgkyl tlnsptvnlr lksldekyyh
      361 hagwdayfag yifiklghvf gvkiygsgle erpithselm ssvknyvnsv nvsrsnemcm
      421 kldgddplys kpewlhvklk sasidtkqlv erlssfgqvd impfarkrvl vavsnqkrns
      481 iaslnnsasd nkctqilqrh hssfisskin lnsienkyar cwpsy
//
LOCUS       XP_034191022             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609155 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191022
VERSION     XP_034191022.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC117609155 isoform X1"
                     /calculated_mol_wt=54462
     Region          75..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..>400
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          96..120
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          121..144
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          169..192
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          195..218
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          219..242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          243..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          292..340
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          341..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          365..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          369..>452
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     CDS             1..484
                     /gene="LOC117609155"
                     /coded_by="XM_034335131.2:1120..2574"
                     /db_xref="GeneID:117609155"
ORIGIN      
        1 mkeqrlwtvi cmlclvmgls laqtsqlcps haeispcsct lkksgldivc eftdlthisk
       61 vmsslkgrtn tvifylrlrh nslpklqpyv flgldirhlt ihnsslakle esslssigtg
      121 ltqldlsqna llsvpssalk dlqhllilnl nrnkikaihk kafegldtle ilslyeneis
      181 tveedafkgl hnrklrrlnl ggnelnkvpt qalrtldmlk klemqenrit aieegdfegl
      241 ksldslglah nqlrevparv fahltqlnsl emdgnqithv dpdafiglee nlqylrlgdn
      301 nlhavpsdal rrlhrlrhld lrannitvlp edaftgygds isflnlqknl ikvlpplvfe
      361 nlnsletlnl qnnkltqipe evtenivdtl rhiditdnpl icdcelrwys vwlgnlrdkd
      421 dermsrkrtv ctmisehrey svqnlplerm gcvgknagrt ssagsscmyv dtmlqilaaa
      481 ivlf
//
LOCUS       XP_034191023             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609155 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191023
VERSION     XP_034191023.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335132.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC117609155 isoform X1"
                     /calculated_mol_wt=54462
     Region          75..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..>400
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          96..120
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          121..144
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          169..192
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          195..218
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          219..242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          243..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          292..340
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          341..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          365..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          369..>452
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     CDS             1..484
                     /gene="LOC117609155"
                     /coded_by="XM_034335132.2:394..1848"
                     /db_xref="GeneID:117609155"
ORIGIN      
        1 mkeqrlwtvi cmlclvmgls laqtsqlcps haeispcsct lkksgldivc eftdlthisk
       61 vmsslkgrtn tvifylrlrh nslpklqpyv flgldirhlt ihnsslakle esslssigtg
      121 ltqldlsqna llsvpssalk dlqhllilnl nrnkikaihk kafegldtle ilslyeneis
      181 tveedafkgl hnrklrrlnl ggnelnkvpt qalrtldmlk klemqenrit aieegdfegl
      241 ksldslglah nqlrevparv fahltqlnsl emdgnqithv dpdafiglee nlqylrlgdn
      301 nlhavpsdal rrlhrlrhld lrannitvlp edaftgygds isflnlqknl ikvlpplvfe
      361 nlnsletlnl qnnkltqipe evtenivdtl rhiditdnpl icdcelrwys vwlgnlrdkd
      421 dermsrkrtv ctmisehrey svqnlplerm gcvgknagrt ssagsscmyv dtmlqilaaa
      481 ivlf
//
LOCUS       XP_034191024             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609155 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191024
VERSION     XP_034191024.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335133.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC117609155 isoform X1"
                     /calculated_mol_wt=54462
     Region          75..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..>400
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          96..120
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          121..144
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          169..192
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          195..218
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          219..242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          243..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          292..340
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          341..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          365..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          369..>452
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     CDS             1..484
                     /gene="LOC117609155"
                     /coded_by="XM_034335133.2:456..1910"
                     /db_xref="GeneID:117609155"
ORIGIN      
        1 mkeqrlwtvi cmlclvmgls laqtsqlcps haeispcsct lkksgldivc eftdlthisk
       61 vmsslkgrtn tvifylrlrh nslpklqpyv flgldirhlt ihnsslakle esslssigtg
      121 ltqldlsqna llsvpssalk dlqhllilnl nrnkikaihk kafegldtle ilslyeneis
      181 tveedafkgl hnrklrrlnl ggnelnkvpt qalrtldmlk klemqenrit aieegdfegl
      241 ksldslglah nqlrevparv fahltqlnsl emdgnqithv dpdafiglee nlqylrlgdn
      301 nlhavpsdal rrlhrlrhld lrannitvlp edaftgygds isflnlqknl ikvlpplvfe
      361 nlnsletlnl qnnkltqipe evtenivdtl rhiditdnpl icdcelrwys vwlgnlrdkd
      421 dermsrkrtv ctmisehrey svqnlplerm gcvgknagrt ssagsscmyv dtmlqilaaa
      481 ivlf
//
LOCUS       XP_034191025             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609155 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191025
VERSION     XP_034191025.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335134.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC117609155 isoform X1"
                     /calculated_mol_wt=54462
     Region          75..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..>400
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          96..120
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          121..144
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          169..192
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          195..218
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          219..242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          243..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          292..340
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          341..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          365..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          369..>452
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     CDS             1..484
                     /gene="LOC117609155"
                     /coded_by="XM_034335134.2:385..1839"
                     /db_xref="GeneID:117609155"
ORIGIN      
        1 mkeqrlwtvi cmlclvmgls laqtsqlcps haeispcsct lkksgldivc eftdlthisk
       61 vmsslkgrtn tvifylrlrh nslpklqpyv flgldirhlt ihnsslakle esslssigtg
      121 ltqldlsqna llsvpssalk dlqhllilnl nrnkikaihk kafegldtle ilslyeneis
      181 tveedafkgl hnrklrrlnl ggnelnkvpt qalrtldmlk klemqenrit aieegdfegl
      241 ksldslglah nqlrevparv fahltqlnsl emdgnqithv dpdafiglee nlqylrlgdn
      301 nlhavpsdal rrlhrlrhld lrannitvlp edaftgygds isflnlqknl ikvlpplvfe
      361 nlnsletlnl qnnkltqipe evtenivdtl rhiditdnpl icdcelrwys vwlgnlrdkd
      421 dermsrkrtv ctmisehrey svqnlplerm gcvgknagrt ssagsscmyv dtmlqilaaa
      481 ivlf
//
LOCUS       XP_034191026             484 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609155 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191026
VERSION     XP_034191026.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335135.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..484
                     /product="uncharacterized protein LOC117609155 isoform X1"
                     /calculated_mol_wt=54462
     Region          75..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..>400
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          96..120
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          121..144
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          169..192
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          195..218
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          219..242
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          243..266
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          267..291
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          292..340
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          341..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          365..385
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          369..>452
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     CDS             1..484
                     /gene="LOC117609155"
                     /coded_by="XM_034335135.2:422..1876"
                     /db_xref="GeneID:117609155"
ORIGIN      
        1 mkeqrlwtvi cmlclvmgls laqtsqlcps haeispcsct lkksgldivc eftdlthisk
       61 vmsslkgrtn tvifylrlrh nslpklqpyv flgldirhlt ihnsslakle esslssigtg
      121 ltqldlsqna llsvpssalk dlqhllilnl nrnkikaihk kafegldtle ilslyeneis
      181 tveedafkgl hnrklrrlnl ggnelnkvpt qalrtldmlk klemqenrit aieegdfegl
      241 ksldslglah nqlrevparv fahltqlnsl emdgnqithv dpdafiglee nlqylrlgdn
      301 nlhavpsdal rrlhrlrhld lrannitvlp edaftgygds isflnlqknl ikvlpplvfe
      361 nlnsletlnl qnnkltqipe evtenivdtl rhiditdnpl icdcelrwys vwlgnlrdkd
      421 dermsrkrtv ctmisehrey svqnlplerm gcvgknagrt ssagsscmyv dtmlqilaaa
      481 ivlf
//
LOCUS       XP_034191027             483 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609155 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191027
VERSION     XP_034191027.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335136.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="uncharacterized protein LOC117609155 isoform X2"
                     /calculated_mol_wt=54320
     Region          75..95
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          96..>399
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          96..120
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          121..144
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          145..168
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          169..193
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          194..217
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          218..241
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          242..265
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          266..290
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          291..339
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          340..363
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          364..384
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          368..>451
                     /region_name="PCC"
                     /note="polycystin cation channel protein; TIGR00864"
                     /db_xref="CDD:188093"
     CDS             1..483
                     /gene="LOC117609155"
                     /coded_by="XM_034335136.2:1124..2575"
                     /db_xref="GeneID:117609155"
ORIGIN      
        1 mkeqrlwtvi cmlclvmgls laqtsqlcps haeispcsct lkksgldivc eftdlthisk
       61 vmsslkgrtn tvifylrlrh nslpklqpyv flgldirhlt ihnsslakle esslssigtg
      121 ltqldlsqna llsvpssalk dlqhllilnl nrnkikaihk kafegldtle ilslyeneis
      181 tveedafkgl hkklrrlnlg gnelnkvptq alrtldmlkk lemqenrita ieegdfeglk
      241 sldslglahn qlrevparvf ahltqlnsle mdgnqithvd pdafigleen lqylrlgdnn
      301 lhavpsdalr rlhrlrhldl rannitvlpe daftgygdsi sflnlqknli kvlpplvfen
      361 lnsletlnlq nnkltqipee vtenivdtlr hiditdnpli cdcelrwysv wlgnlrdkdd
      421 ermsrkrtvc tmisehreys vqnlplermg cvgknagrts sagsscmyvd tmlqilaaai
      481 vlf
//
LOCUS       XP_034191028             450 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin alpha-1 chain [Osmia lignaria lignaria].
ACCESSION   XP_034191028
VERSION     XP_034191028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..450
                     /product="tubulin alpha-1 chain"
                     /calculated_mol_wt=49848
     Region          1..439
                     /region_name="PTZ00335"
                     /note="tubulin alpha chain; Provisional"
                     /db_xref="CDD:185562"
     CDS             1..450
                     /gene="LOC117609156"
                     /coded_by="XM_034335137.2:154..1506"
                     /db_xref="GeneID:117609156"
ORIGIN      
        1 mrecisihvg qagvqignac welyclehgi qpdgqmpsdk tigggddsfn tffsetgagk
       61 hvpravfidl eptvvdevrt gtyrqlfhpe qlitgkedaa nnyarghyti gkeivdlvld
      121 rirkladqct glqgflifhs fgggtgsgft sllmerlsvd ygkksklefa iypapqvsta
      181 vvepynsilt thttlehsdc afmvdneaiy dicrrnldie rptytnlnrl igqivssita
      241 slrfdgalnv dltefqtnlv pyprihfplv tyapvisaek ayheqlsvae itnacfepan
      301 qmvkcdprhg kymaccmlyr gdvvpkdvna aiatiktkrt iqfvdwcptg fkvginyqpp
      361 tvvpggdlak vqravcmlsn ttaiaeawar ldhkfdlmya krafvhwyvg egmeegefse
      421 aredlaalek dyeevgmdsa egeaegaeey
//
LOCUS       XP_034191029             318 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609157 [Osmia lignaria lignaria].
ACCESSION   XP_034191029
VERSION     XP_034191029.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191029.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="uncharacterized protein LOC117609157"
                     /calculated_mol_wt=36229
     CDS             1..318
                     /gene="LOC117609157"
                     /coded_by="XM_034335138.2:129..1085"
                     /db_xref="GeneID:117609157"
ORIGIN      
        1 mscltglysq tltllrvphe ifflqhkvfr eyarvtvgpd fihtlpsfli lssvaillwr
       61 nppscnslaq aitstilyka lqvlvswvva iavlwlwlil qrllycsvwa fwsyeseyrd
      121 vsyqwwqriw ssyypppsqp pvmsagfisw iismliaivt lgcayysnrv wnllsdsmig
      181 lkdtivlisc yensylrril aqshwlqees aklkngssqn dseasstcde crsemssemn
      241 hlpqtqvhpi sygtnwmpkp sfsgfkdpqk kvsmkslhtv avvndiqdsd dlsirqlryk
      301 rgchtvipsn ssdqssgc
//
LOCUS       XP_034186344             284 aa            linear   INV 03-SEP-2025
DEFINITION  cellular Repressor of E1A-stimulated Genes [Osmia lignaria
            lignaria].
ACCESSION   XP_034186344
VERSION     XP_034186344.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330453.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="cellular Repressor of E1A-stimulated Genes"
                     /calculated_mol_wt=33073
     Region          76..244
                     /region_name="Pyrid_oxidase_2"
                     /note="Pyridoxamine 5'-phosphate oxidase; pfam13883"
                     /db_xref="CDD:404723"
     CDS             1..284
                     /gene="CREG"
                     /coded_by="XM_034330453.2:140..994"
                     /db_xref="GeneID:117607157"
ORIGIN      
        1 miwritlfva lfvltieakk ysqsfeehqw qefeeflkwk rtreqhenky ekrhdkshev
       61 dqlyekvqqi plsnpppvde aalmaryivn qadwvsvati strkeiesfp avtlvsysdg
      121 ilgngtgvpy lyltpldfta qdlakdnrvs mlmslaqgdy cknkqwdpmd prcarvlmsg
      181 riktlknesa elelvkklff drhpklvnmp enhhfyfakl kimsiavldt fggpkyvpvn
      241 eylhpsgsni ieefnrrfpl edyeksskdy nsvsnmlhpm irti
//
LOCUS       XP_034190920             881 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190920
VERSION     XP_034190920.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335029.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..881
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X1"
                     /calculated_mol_wt=99550
     Region          68..261
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(81,83..84,86..87,90..93,175,177..178,261)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          268..370
                     /region_name="IPT_NFkappaB"
                     /note="IPT domain of the transcription factor NFkappaB and
                     related transcription factors. NFkappaB is considered a
                     central regulator of stress responses, activated by
                     different stressful conditions, including physical stress,
                     oxidative stress, and exposure to...; cd01177"
                     /db_xref="CDD:238582"
     Site            order(269,271..273,275..277,318..320,333,345,364,367,369)
                     /site_type="other"
                     /note="ankyrin protein binding site [polypeptide binding]"
                     /db_xref="CDD:238582"
     Site            order(271..272,274,287,289,291,325..326,329,331)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238582"
     Site            order(292,294,326..327)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238582"
     Region          420..>589
                     /region_name="PHA03100"
                     /note="ankyrin repeat protein; Provisional; cl39094"
                     /db_xref="CDD:476869"
     Region          <506..723
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          506..544
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          546..577
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          615..646
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(616,620..621,624..626,628..629,633,636,646,648,650,
                     654..655,658..660,662..663,667,670,680,682,684,688..689,
                     692..694,699..700,704,707,716)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          648..680
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          682..716
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          802..873
                     /region_name="DD"
                     /note="Death Domain Superfamily of protein-protein
                     interaction domains; cl14633"
                     /db_xref="CDD:472698"
     CDS             1..881
                     /gene="Rel"
                     /coded_by="XM_034335029.2:142..2787"
                     /db_xref="GeneID:117609121"
ORIGIN      
        1 mpevyenymv lenvsenrnf fpyeydlqtm naqsmcspfs esqrtssmsp ltenefmslk
       61 nataigephl iileqpmekf rfryksemig thgslvgsns nssrkknapt vqlnnfmeta
      121 iirctlvtsd egrriphahr lvyrdggvdy depyhvkvsp etgyivafhg mgiihtakkh
      181 vrdelirkmr iealernkrs nikatistre eaqikadaey yqkcmnlnsv alcfqafivd
      241 ndnvmrpite pvyshtinnl ksaltgelki cridkhtscv egneevfilv ekvgkkniki
      301 kffelnendc evwcaygrft eldvhhqyai vfrtpaykdl nitspkevfi qlerpsdaec
      361 sepikftykp sdriigkkrq rmsysgssel shivptynsp vvdniseils gdsrdvsrel
      421 kkllsegcss plhknlfeci dyeqylpfie ngdenmltsd gplirqhqdd tmfpknilte
      481 vikcmkmnse nmkeyipkll kdrstygdsp lhaalrygqh divkyllmli sadkdckslv
      541 ngqnssgktp lhyavlqnlp evtkallmlg adpnridehg fsplhaavri peggacvdal
      601 lsektvniel pndagwralh laaeagsyda vcflvqagan vndtemsygr talhiavegg
      661 hkniveyllk ktnisvnkrn fsgntalhta vvhsgarake lcalliryga dphiqnhnre
      721 tdnvdkrkgp sinikeeids edentegttg qssfdlamnk pdilqlfngk tedtanqmcs
      781 vatkleiedk nlltnwlnne hkeklstlld ktqgwrklak hlnfeyflkt feqsssspsl
      841 lllnyidvqa slslidmekl lrdtgeeeaa nyvheissty t
//
LOCUS       XP_034190921             852 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190921
VERSION     XP_034190921.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335030.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..852
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X2"
                     /calculated_mol_wt=95933
     Region          39..232
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Site            order(52,54..55,57..58,61..64,146,148..149,232)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143640"
     Region          239..341
                     /region_name="IPT_NFkappaB"
                     /note="IPT domain of the transcription factor NFkappaB and
                     related transcription factors. NFkappaB is considered a
                     central regulator of stress responses, activated by
                     different stressful conditions, including physical stress,
                     oxidative stress, and exposure to...; cd01177"
                     /db_xref="CDD:238582"
     Site            order(240,242..244,246..248,289..291,304,316,335,338,340)
                     /site_type="other"
                     /note="ankyrin protein binding site [polypeptide binding]"
                     /db_xref="CDD:238582"
     Site            order(242..243,245,258,260,262,296..297,300,302)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238582"
     Site            order(263,265,297..298)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238582"
     Region          391..>560
                     /region_name="PHA03100"
                     /note="ankyrin repeat protein; Provisional; cl39094"
                     /db_xref="CDD:476869"
     Region          <477..694
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          477..515
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          517..548
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          586..617
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(587,591..592,595..597,599..600,604,607,617,619,621,
                     625..626,629..631,633..634,638,641,651,653,655,659..660,
                     663..665,670..671,675,678,687)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          619..651
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          653..687
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          773..844
                     /region_name="DD"
                     /note="Death Domain Superfamily of protein-protein
                     interaction domains; cl14633"
                     /db_xref="CDD:472698"
     CDS             1..852
                     /gene="Rel"
                     /coded_by="XM_034335030.2:290..2848"
                     /db_xref="GeneID:117609121"
ORIGIN      
        1 mnaqsmcspf sesqrtssms pltenefmsl knataigeph liileqpmek frfryksemi
       61 gthgslvgsn snssrkknap tvqlnnfmet aiirctlvts degrriphah rlvyrdggvd
      121 ydepyhvkvs petgyivafh gmgiihtakk hvrdelirkm riealernkr snikatistr
      181 eeaqikadae yyqkcmnlns valcfqafiv dndnvmrpit epvyshtinn lksaltgelk
      241 icridkhtsc vegneevfil vekvgkknik ikffelnend cevwcaygrf teldvhhqya
      301 ivfrtpaykd lnitspkevf iqlerpsdae csepikftyk psdriigkkr qrmsysgsse
      361 lshivptyns pvvdniseil sgdsrdvsre lkkllsegcs splhknlfec idyeqylpfi
      421 engdenmlts dgplirqhqd dtmfpknilt evikcmkmns enmkeyipkl lkdrstygds
      481 plhaalrygq hdivkyllml isadkdcksl vngqnssgkt plhyavlqnl pevtkallml
      541 gadpnrideh gfsplhaavr ipeggacvda llsektvnie lpndagwral hlaaeagsyd
      601 avcflvqaga nvndtemsyg rtalhiaveg ghkniveyll kktnisvnkr nfsgntalht
      661 avvhsgarak elcalliryg adphiqnhnr etdnvdkrkg psinikeeid sedentegtt
      721 gqssfdlamn kpdilqlfng ktedtanqmc svatkleied knlltnwlnn ehkeklstll
      781 dktqgwrkla khlnfeyflk tfeqssssps llllnyidvq aslslidmek llrdtgeeea
      841 anyvheisst yt
//
LOCUS       XP_034190922             776 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear factor NF-kappa-B family member relish isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034190922
VERSION     XP_034190922.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335031.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..776
                     /product="nuclear factor NF-kappa-B family member relish
                     isoform X3"
                     /calculated_mol_wt=87690
     Region          <8..156
                     /region_name="RHD-n"
                     /note="N-terminal sub-domain of the Rel homology domain
                     (RHD); cl08275"
                     /db_xref="CDD:447596"
     Region          163..265
                     /region_name="IPT_NFkappaB"
                     /note="IPT domain of the transcription factor NFkappaB and
                     related transcription factors. NFkappaB is considered a
                     central regulator of stress responses, activated by
                     different stressful conditions, including physical stress,
                     oxidative stress, and exposure to...; cd01177"
                     /db_xref="CDD:238582"
     Site            order(164,166..168,170..172,213..215,228,240,259,262,264)
                     /site_type="other"
                     /note="ankyrin protein binding site [polypeptide binding]"
                     /db_xref="CDD:238582"
     Site            order(166..167,169,182,184,186,220..221,224,226)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:238582"
     Site            order(187,189,221..222)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238582"
     Region          315..>484
                     /region_name="PHA03100"
                     /note="ankyrin repeat protein; Provisional; cl39094"
                     /db_xref="CDD:476869"
     Region          <401..618
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          401..439
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          441..472
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          510..541
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(511,515..516,519..521,523..524,528,531,541,543,545,
                     549..550,553..555,557..558,562,565,575,577,579,583..584,
                     587..589,594..595,599,602,611)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          543..575
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          577..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          697..768
                     /region_name="DD"
                     /note="Death Domain Superfamily of protein-protein
                     interaction domains; cl14633"
                     /db_xref="CDD:472698"
     CDS             1..776
                     /gene="Rel"
                     /coded_by="XM_034335031.2:126..2456"
                     /db_xref="GeneID:117609121"
ORIGIN      
        1 mhqqykylnn fmetaiirct lvtsdegrri phahrlvyrd ggvdydepyh vkvspetgyi
       61 vafhgmgiih takkhvrdel irkmrieale rnkrsnikat istreeaqik adaeyyqkcm
      121 nlnsvalcfq afivdndnvm rpitepvysh tinnlksalt gelkicridk htscvegnee
      181 vfilvekvgk knikikffel nendcevwca ygrfteldvh hqyaivfrtp aykdlnitsp
      241 kevfiqlerp sdaecsepik ftykpsdrii gkkrqrmsys gsselshivp tynspvvdni
      301 seilsgdsrd vsrelkklls egcssplhkn lfecidyeqy lpfiengden mltsdgplir
      361 qhqddtmfpk niltevikcm kmnsenmkey ipkllkdrst ygdsplhaal rygqhdivky
      421 llmlisadkd ckslvngqns sgktplhyav lqnlpevtka llmlgadpnr idehgfsplh
      481 aavripegga cvdallsekt vnielpndag wralhlaaea gsydavcflv qaganvndte
      541 msygrtalhi avegghkniv eyllkktnis vnkrnfsgnt alhtavvhsg arakelcall
      601 irygadphiq nhnretdnvd krkgpsinik eeidsedent egttgqssfd lamnkpdilq
      661 lfngktedta nqmcsvatkl eiedknlltn wlnnehkekl stlldktqgw rklakhlnfe
      721 yflktfeqss sspsllllny idvqaslsli dmekllrdtg eeeaanyvhe isstyt
//
LOCUS       XP_076548081             776 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent RNA helicase DDX54 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548081
VERSION     XP_076548081.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691966.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..776
                     /product="ATP-dependent RNA helicase DDX54 isoform X1"
                     /calculated_mol_wt=87687
     Region          39..243
                     /region_name="DEADc_DDX54"
                     /note="DEAD-box helicase domain of DEAD box protein 54;
                     cd17959"
                     /db_xref="CDD:350717"
     Site            order(58..59,61..63,66,84..91)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350717"
     Region          254..385
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(344,346,371,374..375)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          635..695
                     /region_name="DBP10CT"
                     /note="DBP10CT (NUC160) domain; pfam08147"
                     /db_xref="CDD:462373"
     CDS             1..776
                     /gene="LOC117609122"
                     /coded_by="XM_076691966.1:256..2586"
                     /db_xref="GeneID:117609122"
ORIGIN      
        1 mlrmkdthie gfadpeeind neeeneitni kkkvckksgg fqsmalsypv lkgilkrgyk
       61 iptpiqrkti plalegrdvv amartgsgkt acfliplfek lkarqaktga ralilsptre
      121 lalqtlkfik elgrftglqa avilggdsme nqfstihgnp dilvatpgrf lhlcvemdlq
      181 lnsieyvvfd eadrlfemgf geqineitnr lpecrqtllf satlpkvlvd fakagltdpv
      241 llrldvdski peeltlsfiv crpeeklavl lcllkniikd dsqtvifaat mhhveyvhmv
      301 ldkagisntf iysnldpsar kinaakfqtg kvkvlvvtdv aargldiphl dnvinfnfpa
      361 ksklfvhrvg rcaragrtgt ayniigpdey aylvdlhlfl srplcvipss gsvkntdgai
      421 gkmpqsmiee elaelinwhn sstdltnmen vcenaykqyi rsrpaasses ikrvkelhin
      481 qagvipeysd vssntmnlls kivnyrpqgt ifeigakass tdyqvmktkr tlhkqniqnf
      541 yrkaeeltsk knsedllkkv nlpsstadei hatfntivlp kkrnpddlyk ppkkkkrtvt
      601 rdeefyipys apdkhteegl avnsfnteae kvqmdltadn eesqrlhaql kkwdrkkkkm
      661 vtinndtkak kirtesgvwi pasyksnrys awkekskada asdndseeet pqmrklkttf
      721 nthwarhnqk tkeklkgnre lkrpeqilka rklleqkrkr ndrkgrkgqk kggkkr
//
LOCUS       XP_034190924             776 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent RNA helicase DDX54 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190924
VERSION     XP_034190924.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335033.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..776
                     /product="ATP-dependent RNA helicase DDX54 isoform X1"
                     /calculated_mol_wt=87687
     Region          39..243
                     /region_name="DEADc_DDX54"
                     /note="DEAD-box helicase domain of DEAD box protein 54;
                     cd17959"
                     /db_xref="CDD:350717"
     Site            order(58..59,61..63,66,84..91)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350717"
     Region          254..385
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(344,346,371,374..375)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          635..695
                     /region_name="DBP10CT"
                     /note="DBP10CT (NUC160) domain; pfam08147"
                     /db_xref="CDD:462373"
     CDS             1..776
                     /gene="LOC117609122"
                     /coded_by="XM_034335033.2:153..2483"
                     /db_xref="GeneID:117609122"
ORIGIN      
        1 mlrmkdthie gfadpeeind neeeneitni kkkvckksgg fqsmalsypv lkgilkrgyk
       61 iptpiqrkti plalegrdvv amartgsgkt acfliplfek lkarqaktga ralilsptre
      121 lalqtlkfik elgrftglqa avilggdsme nqfstihgnp dilvatpgrf lhlcvemdlq
      181 lnsieyvvfd eadrlfemgf geqineitnr lpecrqtllf satlpkvlvd fakagltdpv
      241 llrldvdski peeltlsfiv crpeeklavl lcllkniikd dsqtvifaat mhhveyvhmv
      301 ldkagisntf iysnldpsar kinaakfqtg kvkvlvvtdv aargldiphl dnvinfnfpa
      361 ksklfvhrvg rcaragrtgt ayniigpdey aylvdlhlfl srplcvipss gsvkntdgai
      421 gkmpqsmiee elaelinwhn sstdltnmen vcenaykqyi rsrpaasses ikrvkelhin
      481 qagvipeysd vssntmnlls kivnyrpqgt ifeigakass tdyqvmktkr tlhkqniqnf
      541 yrkaeeltsk knsedllkkv nlpsstadei hatfntivlp kkrnpddlyk ppkkkkrtvt
      601 rdeefyipys apdkhteegl avnsfnteae kvqmdltadn eesqrlhaql kkwdrkkkkm
      661 vtinndtkak kirtesgvwi pasyksnrys awkekskada asdndseeet pqmrklkttf
      721 nthwarhnqk tkeklkgnre lkrpeqilka rklleqkrkr ndrkgrkgqk kggkkr
//
LOCUS       XP_034190925             773 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent RNA helicase DDX54 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190925
VERSION     XP_034190925.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..773
                     /product="ATP-dependent RNA helicase DDX54 isoform X2"
                     /calculated_mol_wt=87287
     Region          36..240
                     /region_name="DEADc_DDX54"
                     /note="DEAD-box helicase domain of DEAD box protein 54;
                     cd17959"
                     /db_xref="CDD:350717"
     Site            order(55..56,58..60,63,81..88)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350717"
     Region          251..382
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(341,343,368,371..372)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          632..692
                     /region_name="DBP10CT"
                     /note="DBP10CT (NUC160) domain; pfam08147"
                     /db_xref="CDD:462373"
     CDS             1..773
                     /gene="LOC117609122"
                     /coded_by="XM_034335034.2:183..2504"
                     /db_xref="GeneID:117609122"
ORIGIN      
        1 mkdthiegfa dpeeindnee eneitnikkk vckksggfqs malsypvlkg ilkrgykipt
       61 piqrktipla legrdvvama rtgsgktacf liplfeklka rqaktgaral ilsptrelal
      121 qtlkfikelg rftglqaavi lggdsmenqf stihgnpdil vatpgrflhl cvemdlqlns
      181 ieyvvfdead rlfemgfgeq ineitnrlpe crqtllfsat lpkvlvdfak agltdpvllr
      241 ldvdskipee ltlsfivcrp eeklavllcl lkniikddsq tvifaatmhh veyvhmvldk
      301 agisntfiys nldpsarkin aakfqtgkvk vlvvtdvaar gldiphldnv infnfpaksk
      361 lfvhrvgrca ragrtgtayn iigpdeyayl vdlhlflsrp lcvipssgsv kntdgaigkm
      421 pqsmieeela elinwhnsst dltnmenvce naykqyirsr paassesikr vkelhinqag
      481 vipeysdvss ntmnllskiv nyrpqgtife igakasstdy qvmktkrtlh kqniqnfyrk
      541 aeeltskkns edllkkvnlp sstadeihat fntivlpkkr npddlykppk kkkrtvtrde
      601 efyipysapd khteeglavn sfnteaekvq mdltadnees qrlhaqlkkw drkkkkmvti
      661 nndtkakkir tesgvwipas yksnrysawk ekskadaasd ndseeetpqm rklkttfnth
      721 warhnqktke klkgnrelkr peqilkarkl leqkrkrndr kgrkgqkkgg kkr
//
LOCUS       XP_034190926             773 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent RNA helicase DDX54 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190926
VERSION     XP_034190926.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335035.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..773
                     /product="ATP-dependent RNA helicase DDX54 isoform X2"
                     /calculated_mol_wt=87287
     Region          36..240
                     /region_name="DEADc_DDX54"
                     /note="DEAD-box helicase domain of DEAD box protein 54;
                     cd17959"
                     /db_xref="CDD:350717"
     Site            order(55..56,58..60,63,81..88)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350717"
     Region          251..382
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(341,343,368,371..372)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          632..692
                     /region_name="DBP10CT"
                     /note="DBP10CT (NUC160) domain; pfam08147"
                     /db_xref="CDD:462373"
     CDS             1..773
                     /gene="LOC117609122"
                     /coded_by="XM_034335035.2:116..2437"
                     /db_xref="GeneID:117609122"
ORIGIN      
        1 mkdthiegfa dpeeindnee eneitnikkk vckksggfqs malsypvlkg ilkrgykipt
       61 piqrktipla legrdvvama rtgsgktacf liplfeklka rqaktgaral ilsptrelal
      121 qtlkfikelg rftglqaavi lggdsmenqf stihgnpdil vatpgrflhl cvemdlqlns
      181 ieyvvfdead rlfemgfgeq ineitnrlpe crqtllfsat lpkvlvdfak agltdpvllr
      241 ldvdskipee ltlsfivcrp eeklavllcl lkniikddsq tvifaatmhh veyvhmvldk
      301 agisntfiys nldpsarkin aakfqtgkvk vlvvtdvaar gldiphldnv infnfpaksk
      361 lfvhrvgrca ragrtgtayn iigpdeyayl vdlhlflsrp lcvipssgsv kntdgaigkm
      421 pqsmieeela elinwhnsst dltnmenvce naykqyirsr paassesikr vkelhinqag
      481 vipeysdvss ntmnllskiv nyrpqgtife igakasstdy qvmktkrtlh kqniqnfyrk
      541 aeeltskkns edllkkvnlp sstadeihat fntivlpkkr npddlykppk kkkrtvtrde
      601 efyipysapd khteeglavn sfnteaekvq mdltadnees qrlhaqlkkw drkkkkmvti
      661 nndtkakkir tesgvwipas yksnrysawk ekskadaasd ndseeetpqm rklkttfnth
      721 warhnqktke klkgnrelkr peqilkarkl leqkrkrndr kgrkgqkkgg kkr
//
LOCUS       XP_034190927             317 aa            linear   INV 03-SEP-2025
DEFINITION  beta Hydroxy acid dehydrogenase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034190927
VERSION     XP_034190927.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335036.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="beta Hydroxy acid dehydrogenase 1"
                     /calculated_mol_wt=35489
     Region          18..311
                     /region_name="PRK06129"
                     /note="3-hydroxyacyl-CoA dehydrogenase; Validated"
                     /db_xref="CDD:235706"
     CDS             1..317
                     /gene="Had1"
                     /coded_by="XM_034335036.2:124..1077"
                     /db_xref="GeneID:117609123"
ORIGIN      
        1 msyknekigi igsgligrsw amlfasvgye vtiydivkeq itralddihq qltrlesggl
       61 lrgtlnadqq fklikgssnl aesvkgakli qecvpenlsl klkvyneldn vvdnkvilss
      121 ststfrpslf seklkhrdqi ivshpvnppy yvplveivpa pwtraeiplq tkaimteigq
      181 kpvvfsreid gfalnriqya ilneawrlva dgilsakdvd avmseglgmr yaflgsfeaa
      241 hlnaegmkky cetyknsiyd vsmtfgpvpk fegemaekis nelnemcpld klqerrawrd
      301 ealtklsllk kelaqkk
//
LOCUS       XP_034190928             154 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein L24 [Osmia lignaria lignaria].
ACCESSION   XP_034190928
VERSION     XP_034190928.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335037.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..154
                     /product="ribosomal protein L24"
                     /calculated_mol_wt=17336
     Region          2..63
                     /region_name="Ribosomal_L24e"
                     /note="Ribosomal protein L24e; pfam01246"
                     /db_xref="CDD:460131"
     CDS             1..154
                     /gene="RpL24"
                     /coded_by="XM_034335037.1:102..566"
                     /db_xref="GeneID:117609124"
ORIGIN      
        1 mkiglcaysg ykiypghgkt mvkvdgktft flnskcesah lmrrnprkvt wtvlyrrkhk
       61 kgqeeeqakk rtrrtqkfqr aivgasltdi lakrnmkpei rkaqreqaik aakeqkkavk
      121 atkkavvapk akalpkhkaa kvtqksaprv ggkr
//
LOCUS       XP_034182937            1051 aa            linear   INV 03-SEP-2025
DEFINITION  sodium chloride cotransporter 69 [Osmia lignaria lignaria].
ACCESSION   XP_034182937
VERSION     XP_034182937.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327046.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182937.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1051
                     /product="sodium chloride cotransporter 69"
                     /calculated_mol_wt=116351
     Region          54..1051
                     /region_name="AA_permease_2"
                     /note="Amino acid permease; cl45918"
                     /db_xref="CDD:459263"
     CDS             1..1051
                     /gene="Ncc69"
                     /coded_by="XM_034327046.2:380..3535"
                     /db_xref="GeneID:117605568"
ORIGIN      
        1 mahenglngd tdsetvelnp lrrtrfhvnr vdslegrasl lgeqetkksl rhmtrealpr
       61 ldnyrnimsi qaahrpslde lhnptllnks sgshtlpiah vkppstgvkf gwiqgvllrc
      121 llniwgvmlf lrlswvvaqa gigeaillil tttvvttits lsmsaistng likgggtyym
      181 isrslgpefg gsiglifsla navacsmyvv gfcesmvdcl ksngvcivdc dntdiriigc
      241 itialllliv migleweaka qigllvilll aivdfmigtf igpkddlera kgfigynadl
      301 ftenfqsdyr ysegvqhnff svlaiffpaa tgilaganis gdlkdpqtai pkgtllaill
      361 ttisylfmal mvggtvsrda sgdvndlwnv ynssynvlss fnatnetaes piatanllns
      421 sqitwtaygt kfnctgncty gshnsfqvie lvsafgpfiy agcfaatlss alaslvsapk
      481 vfqalcldel ypgiswfsge knkepirgyl ltfviavgfi ligelnaiap lisnfflaay
      541 tlvnfstfha slakpigwrp tfkyynmwls lvgailcvsv mfliswwtal itlsvvlaly
      601 lvvsyrkpdv nwgsttqaqt ynnaltavqq ldrveehvkn yrpqllvltg ipstrsalvd
      661 fahhitknns lficghvvet pisyktrnsm iekctswfra nkikafysvv dganfqdgtt
      721 tllqaaglgk mkpnillmgy kqdwatcpre qlnmyfnvmh kaldmhiava llrltegldc
      781 satigdndeq krntsttlpg nqsfsqlsqa sstsdisipg spaprrskvi neypnptted
      841 qrenrqtiip iakdilnvvt kfqrkqkkgt idvwwlyddg gltlllpyii strrnwsnsk
      901 lrvfalankh seleyeqrnm asllskfrid ysalkvipdi skpaqestkn ffnllienfq
      961 qtedpkatdd dmikesella mkektnrhlr lrelllenst eanlvvmtlp mprkgavsap
     1021 lymawletlt rdmppfllvr gnhtsvltfy s
//
LOCUS       XP_034182938            1051 aa            linear   INV 03-SEP-2025
DEFINITION  sodium chloride cotransporter 69 [Osmia lignaria lignaria].
ACCESSION   XP_034182938
VERSION     XP_034182938.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327047.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182938.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1051
                     /product="sodium chloride cotransporter 69"
                     /calculated_mol_wt=116351
     Region          54..1051
                     /region_name="AA_permease_2"
                     /note="Amino acid permease; cl45918"
                     /db_xref="CDD:459263"
     CDS             1..1051
                     /gene="Ncc69"
                     /coded_by="XM_034327047.2:381..3536"
                     /db_xref="GeneID:117605568"
ORIGIN      
        1 mahenglngd tdsetvelnp lrrtrfhvnr vdslegrasl lgeqetkksl rhmtrealpr
       61 ldnyrnimsi qaahrpslde lhnptllnks sgshtlpiah vkppstgvkf gwiqgvllrc
      121 llniwgvmlf lrlswvvaqa gigeaillil tttvvttits lsmsaistng likgggtyym
      181 isrslgpefg gsiglifsla navacsmyvv gfcesmvdcl ksngvcivdc dntdiriigc
      241 itialllliv migleweaka qigllvilll aivdfmigtf igpkddlera kgfigynadl
      301 ftenfqsdyr ysegvqhnff svlaiffpaa tgilaganis gdlkdpqtai pkgtllaill
      361 ttisylfmal mvggtvsrda sgdvndlwnv ynssynvlss fnatnetaes piatanllns
      421 sqitwtaygt kfnctgncty gshnsfqvie lvsafgpfiy agcfaatlss alaslvsapk
      481 vfqalcldel ypgiswfsge knkepirgyl ltfviavgfi ligelnaiap lisnfflaay
      541 tlvnfstfha slakpigwrp tfkyynmwls lvgailcvsv mfliswwtal itlsvvlaly
      601 lvvsyrkpdv nwgsttqaqt ynnaltavqq ldrveehvkn yrpqllvltg ipstrsalvd
      661 fahhitknns lficghvvet pisyktrnsm iekctswfra nkikafysvv dganfqdgtt
      721 tllqaaglgk mkpnillmgy kqdwatcpre qlnmyfnvmh kaldmhiava llrltegldc
      781 satigdndeq krntsttlpg nqsfsqlsqa sstsdisipg spaprrskvi neypnptted
      841 qrenrqtiip iakdilnvvt kfqrkqkkgt idvwwlyddg gltlllpyii strrnwsnsk
      901 lrvfalankh seleyeqrnm asllskfrid ysalkvipdi skpaqestkn ffnllienfq
      961 qtedpkatdd dmikesella mkektnrhlr lrelllenst eanlvvmtlp mprkgavsap
     1021 lymawletlt rdmppfllvr gnhtsvltfy s
//
LOCUS       XP_034182941             715 aa            linear   INV 03-SEP-2025
DEFINITION  dynein intermediate chain 2, ciliary isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182941
VERSION     XP_034182941.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327050.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..715
                     /product="dynein intermediate chain 2, ciliary isoform X1"
                     /calculated_mol_wt=82006
     Region          <390..619
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          394..437
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          442..491
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          507..544
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          551..588
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          593..634
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..715
                     /gene="LOC117605569"
                     /coded_by="XM_034327050.1:111..2258"
                     /db_xref="GeneID:117605569"
ORIGIN      
        1 mpftnirgtv srtpdlvkrq sfmksrvdlg ggdvdwmrgk gflkpedqlq ladaelqeef
       61 arvltihntr vpdsliewsw kfknfiklpp pphlvtllnv pgmilhkdse eakvqlaggl
      121 vtdievpapq kfeeddeaee geeeeeeere egeerdeiqe eelpkpteve aehkepeadq
      181 ehepdeeeie hikpkklpnq fnfceraalt ysnplremst qtvppptaif nthvfqwtif
      241 deyqedfaqq qrerekerri pvvqpkkedl kkkaqtesma lmsrmlqaak tlermvnqni
      301 fdeilqdyry wddpsdefkn gegsllplwk fsyektkkhh vtdlcfnnry ydlfavsygt
      361 msfssmitng aiclfslknp sypewicste spvmclafse qhphllvigt tdgavavyni
      421 mlpptapqyk sndvvqkhgg ivwevcwapd teegnlafys vsidgkinyw ilnqndlglt
      481 tvmtlfldrp pipgpdgtmi tlkgcgtclv fhpadqnifl vgteegtiyk cntayssvym
      541 ktyneahnmp iyrivfnkfn ssifascsgd wrikiweddr meplfmfdlg vpigdvqwap
      601 ysstvlacvs ndgkvtvfdl nvnkyrpics qqivnrkknk ptrlafnnkl pfiivgddkg
      661 tintlklspn lrlkvkptkk qlhlthtele vmklerllsf vreppvlipp kdert
//
LOCUS       XP_034182942             630 aa            linear   INV 03-SEP-2025
DEFINITION  dynein intermediate chain 2, ciliary isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182942
VERSION     XP_034182942.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327051.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..630
                     /product="dynein intermediate chain 2, ciliary isoform X2"
                     /calculated_mol_wt=72188
     Region          <390..612
                     /region_name="WD40"
                     /note="WD40 domain, found in a number of eukaryotic
                     proteins that cover a wide variety of functions including
                     adaptor/regulatory modules in signal transduction,
                     pre-mRNA processing and cytoskeleton assembly; typically
                     contains a GH dipeptide 11-24 residues from...; cl29593"
                     /db_xref="CDD:475233"
     Region          395..437
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          443..501
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          506..544
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     Region          552..588
                     /region_name="WD40 repeat"
                     /note="WD40 repeat [structural motif]"
                     /db_xref="CDD:293791"
     CDS             1..630
                     /gene="LOC117605569"
                     /coded_by="XM_034327051.1:111..2003"
                     /db_xref="GeneID:117605569"
ORIGIN      
        1 mpftnirgtv srtpdlvkrq sfmksrvdlg ggdvdwmrgk gflkpedqlq ladaelqeef
       61 arvltihntr vpdsliewsw kfknfiklpp pphlvtllnv pgmilhkdse eakvqlaggl
      121 vtdievpapq kfeeddeaee geeeeeeere egeerdeiqe eelpkpteve aehkepeadq
      181 ehepdeeeie hikpkklpnq fnfceraalt ysnplremst qtvppptaif nthvfqwtif
      241 deyqedfaqq qrerekerri pvvqpkkedl kkkaqtesma lmsrmlqaak tlermvnqni
      301 fdeilqdyry wddpsdefkn gegsllplwk fsyektkkhh vtdlcfnnry ydlfavsygt
      361 msfssmitng aiclfslknp sypewicste spvmclafse qhphllvigt tdgavavyni
      421 mlpptapqyk sndvvqkhgg ivwevcwapd teegnlafys vsidgkinyw ilnqndlglt
      481 tvmtlfldrp pipgpdgtmi tlkgcgtclv fhpadqnifl vgteegtiyk cntayssvym
      541 ktyneahnmp iyrivfnkfn ssifascsgd wrikiweddr meplfmfdlg vpigdvqwap
      601 ysstvlacvs ndekkinqld lhstinchsl
//
LOCUS       XP_034182943             496 aa            linear   INV 03-SEP-2025
DEFINITION  ribitol 5-phosphate transferase FKRP [Osmia lignaria lignaria].
ACCESSION   XP_034182943
VERSION     XP_034182943.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327052.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..496
                     /product="ribitol 5-phosphate transferase FKRP"
                     /calculated_mol_wt=57260
     Region          343..>397
                     /region_name="LicD"
                     /note="LicD family; cl01378"
                     /db_xref="CDD:470175"
     CDS             1..496
                     /gene="LOC117605570"
                     /coded_by="XM_034327052.2:178..1668"
                     /db_xref="GeneID:117605570"
ORIGIN      
        1 mrfkfvrtll vlallgniiv whriwrlftt qntlrlltps mvtpdppqkl hrrlarlvtv
       61 virqfetfen dvtstvesvl nffptipili vcnelpyppl eldfanesmq nvklvslqpe
      121 fnksndernp lfyirtkyvl ylpdgsrlps kqiiqdtiaq taklgivtip igsitlncin
      181 idlkvkewsl rfshttgnec dsvngkhatm fetkllrklt dpfllpltda lyiqttalsi
      241 kihilsdyhf negkplyrnq qsqwkvqqlh qirerslfek lgikkvirls naiewygcsr
      301 essrcfgsvv ngvpsylyqn rytppcclsg lrkvahhvfd kleevgirfw lesgsllgam
      361 rngdilpwdh evqigvnrdd lerspwlika mnkpvvdnhg fvwekatege ffkvqysrin
      421 hltvnvlpfy akngsmvkda wflnnkdfpe qflhpmstie fagrqvpcpn nirdflelky
      481 frgvienpel pgkisl
//
LOCUS       XP_034183032             362 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-like protein 7 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183032
VERSION     XP_034183032.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327141.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..362
                     /product="ubiquitin-like protein 7 isoform X1"
                     /calculated_mol_wt=39139
     Region          9..79
                     /region_name="ubiquitin"
                     /note="Ubiquitin family; pfam00240"
                     /db_xref="CDD:459726"
     Region          <46..83
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          319..356
                     /region_name="UBA_UBL7"
                     /note="UBA domain found in ubiquitin-like protein 7 (UBL7)
                     and similar proteins; cd14326"
                     /db_xref="CDD:270511"
     CDS             1..362
                     /gene="LOC117605615"
                     /coded_by="XM_034327141.2:373..1461"
                     /db_xref="GeneID:117605615"
ORIGIN      
        1 mtselilgir lepqtlttik lndinfkikv gelkyetakk vnlakelfel iycgsvledd
       61 mtlefygiks gamvhvlrkr eselmslpky isedsilqla stfksfkenp klrsalnhlg
      121 erpevinnii ssspglhedt vaiailqdpd lmayftdvdt vrrfaeahpv lveaaqniaa
      181 avhaevhnnt tagtnsslst lqptaylysl dnlsddeema gdssqssdst qpsnisnnps
      241 nsaitvaqla aavsrarars fplsnspsst sagstnsgvi ttemftqamq qasaatpgli
      301 ndttlppilp pvlpqftdlq rqlaqmhelg lqddtvniqa lhftngdvqa aielvfngfs
      361 nn
//
LOCUS       XP_034183033             302 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-like protein 7 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183033
VERSION     XP_034183033.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..302
                     /product="ubiquitin-like protein 7 isoform X2"
                     /calculated_mol_wt=32313
     Region          <2..23
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          259..296
                     /region_name="UBA_UBL7"
                     /note="UBA domain found in ubiquitin-like protein 7 (UBL7)
                     and similar proteins; cd14326"
                     /db_xref="CDD:270511"
     CDS             1..302
                     /gene="LOC117605615"
                     /coded_by="XM_034327142.2:488..1396"
                     /db_xref="GeneID:117605615"
ORIGIN      
        1 mtlefygiks gamvhvlrkr eselmslpky isedsilqla stfksfkenp klrsalnhlg
       61 erpevinnii ssspglhedt vaiailqdpd lmayftdvdt vrrfaeahpv lveaaqniaa
      121 avhaevhnnt tagtnsslst lqptaylysl dnlsddeema gdssqssdst qpsnisnnps
      181 nsaitvaqla aavsrarars fplsnspsst sagstnsgvi ttemftqamq qasaatpgli
      241 ndttlppilp pvlpqftdlq rqlaqmhelg lqddtvniqa lhftngdvqa aielvfngfs
      301 nn
//
LOCUS       XP_034182945             302 aa            linear   INV 03-SEP-2025
DEFINITION  N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034182945
VERSION     XP_034182945.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327054.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..302
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase 1 isoform X1"
                     /calculated_mol_wt=33097
     Region          15..248
                     /region_name="DdaH"
                     /note="N-Dimethylarginine dimethylaminohydrolase [Amino
                     acid transport and metabolism]; COG1834"
                     /db_xref="CDD:441439"
     CDS             1..302
                     /gene="LOC117605571"
                     /coded_by="XM_034327054.2:155..1063"
                     /db_xref="GeneID:117605571"
ORIGIN      
        1 mplhrythav lcriplslrt rgevtldear tqhlalaqll reldidvvem ppdensplcv
       61 fvediavvcn gialiarpne psrlkeieti ravlkkelei plieiadkna rldggdvlft
      121 greffvglsh ytneagarav aaafpeypcv pikvaeskrl kglvtmagpd iicvgagkea
      181 qevlkriere atyfyrtltv pedvaanvly vngtlihrse deipqsskvf aekvefatrs
      241 lpmselaktn egkrpvfqgk svvtgemeac issftfflsh vkgstngtes lsgiqsphnl
      301 tp
//
LOCUS       XP_034182946             298 aa            linear   INV 03-SEP-2025
DEFINITION  N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034182946
VERSION     XP_034182946.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327055.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase 1 isoform X2"
                     /calculated_mol_wt=32869
     Region          15..248
                     /region_name="DdaH"
                     /note="N-Dimethylarginine dimethylaminohydrolase [Amino
                     acid transport and metabolism]; COG1834"
                     /db_xref="CDD:441439"
     CDS             1..298
                     /gene="LOC117605571"
                     /coded_by="XM_034327055.2:155..1051"
                     /db_xref="GeneID:117605571"
ORIGIN      
        1 mplhrythav lcriplslrt rgevtldear tqhlalaqll reldidvvem ppdensplcv
       61 fvediavvcn gialiarpne psrlkeieti ravlkkelei plieiadkna rldggdvlft
      121 greffvglsh ytneagarav aaafpeypcv pikvaeskrl kglvtmagpd iicvgagkea
      181 qevlkriere atyfyrtltv pedvaanvly vngtlihrse deipqsskvf aekvefatrs
      241 lpmselaktn egkrpvfqgk svvtgemeac issftfflsh vkgstngtes lseyirrv
//
LOCUS       XP_034182947             269 aa            linear   INV 03-SEP-2025
DEFINITION  N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034182947
VERSION     XP_034182947.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327056.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..269
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase 1 isoform X3"
                     /calculated_mol_wt=29710
     Region          15..257
                     /region_name="DdaH"
                     /note="N-Dimethylarginine dimethylaminohydrolase [Amino
                     acid transport and metabolism]; COG1834"
                     /db_xref="CDD:441439"
     CDS             1..269
                     /gene="LOC117605571"
                     /coded_by="XM_034327056.2:150..959"
                     /db_xref="GeneID:117605571"
ORIGIN      
        1 mplhrythav lcriplslrt rgevtldear tqhlalaqll reldidvvem ppdensplcv
       61 fvediavvcn gialiarpne psrlkeieti ravlkkelei plieiadkna rldggdvlft
      121 greffvglsh ytneagarav aaafpeypcv pikvaeskrl kglvtmagpd iicvgagkea
      181 qevlkriere atyfyrtltv pedvaanvly vngtlihrse deipqsskvf aekvefatrs
      241 lpmselakvs sgltsccllv rrprhirsi
//
LOCUS       XP_034182948             253 aa            linear   INV 03-SEP-2025
DEFINITION  N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034182948
VERSION     XP_034182948.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327057.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase 1 isoform X4"
                     /calculated_mol_wt=27452
     Region          <1..199
                     /region_name="DdaH"
                     /note="N-Dimethylarginine dimethylaminohydrolase [Amino
                     acid transport and metabolism]; COG1834"
                     /db_xref="CDD:441439"
     CDS             1..253
                     /gene="LOC117605571"
                     /coded_by="XM_034327057.2:217..978"
                     /db_xref="GeneID:117605571"
ORIGIN      
        1 mppdensplc vfvediavvc ngialiarpn epsrlkeiet iravlkkele iplieiadkn
       61 arldggdvlf tgreffvgls hytneagara vaaafpeypc vpikvaeskr lkglvtmagp
      121 diicvgagke aqevlkrier eatyfyrtlt vpedvaanvl yvngtlihrs edeipqsskv
      181 faekvefatr slpmselakt negkrpvfqg ksvvtgemea cissftffls hvkgstngte
      241 slsgiqsphn ltp
//
LOCUS       XP_034182950             183 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605572 [Osmia lignaria lignaria].
ACCESSION   XP_034182950
VERSION     XP_034182950.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327059.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..183
                     /product="uncharacterized protein LOC117605572"
                     /calculated_mol_wt=20501
     CDS             1..183
                     /gene="LOC117605572"
                     /coded_by="XM_034327059.2:221..772"
                     /db_xref="GeneID:117605572"
ORIGIN      
        1 medsgidsdp kttshveder lflgsdssss snqtqtlqhn sttkqlqrkl earieqakri
       61 qrnsdyvklp kydkgcgggs sttglisiqr lpipnknkeh lplveywhcd sesegemtlf
      121 pinkskdssk hkqdltdtfs ieelseesed slnlgparss pelkfmksyr ctgdccrcqc
      181 hil
//
LOCUS       XP_034182951             183 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605572 [Osmia lignaria lignaria].
ACCESSION   XP_034182951
VERSION     XP_034182951.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327060.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..183
                     /product="uncharacterized protein LOC117605572"
                     /calculated_mol_wt=20501
     CDS             1..183
                     /gene="LOC117605572"
                     /coded_by="XM_034327060.2:307..858"
                     /db_xref="GeneID:117605572"
ORIGIN      
        1 medsgidsdp kttshveder lflgsdssss snqtqtlqhn sttkqlqrkl earieqakri
       61 qrnsdyvklp kydkgcgggs sttglisiqr lpipnknkeh lplveywhcd sesegemtlf
      121 pinkskdssk hkqdltdtfs ieelseesed slnlgparss pelkfmksyr ctgdccrcqc
      181 hil
//
LOCUS       XP_034182952             183 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605572 [Osmia lignaria lignaria].
ACCESSION   XP_034182952
VERSION     XP_034182952.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327061.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..183
                     /product="uncharacterized protein LOC117605572"
                     /calculated_mol_wt=20501
     CDS             1..183
                     /gene="LOC117605572"
                     /coded_by="XM_034327061.2:287..838"
                     /db_xref="GeneID:117605572"
ORIGIN      
        1 medsgidsdp kttshveder lflgsdssss snqtqtlqhn sttkqlqrkl earieqakri
       61 qrnsdyvklp kydkgcgggs sttglisiqr lpipnknkeh lplveywhcd sesegemtlf
      121 pinkskdssk hkqdltdtfs ieelseesed slnlgparss pelkfmksyr ctgdccrcqc
      181 hil
//
LOCUS       XP_076548084             183 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605572 [Osmia lignaria lignaria].
ACCESSION   XP_076548084
VERSION     XP_076548084.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691969.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..183
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..183
                     /product="uncharacterized protein LOC117605572"
                     /calculated_mol_wt=20501
     CDS             1..183
                     /gene="LOC117605572"
                     /coded_by="XM_076691969.1:275..826"
                     /db_xref="GeneID:117605572"
ORIGIN      
        1 medsgidsdp kttshveder lflgsdssss snqtqtlqhn sttkqlqrkl earieqakri
       61 qrnsdyvklp kydkgcgggs sttglisiqr lpipnknkeh lplveywhcd sesegemtlf
      121 pinkskdssk hkqdltdtfs ieelseesed slnlgparss pelkfmksyr ctgdccrcqc
      181 hil
//
LOCUS       XP_034186471             662 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607197 [Osmia lignaria lignaria].
ACCESSION   XP_034186471
VERSION     XP_034186471.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330580.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186471.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..662
                     /product="uncharacterized protein LOC117607197"
                     /calculated_mol_wt=71887
     CDS             1..662
                     /gene="LOC117607197"
                     /coded_by="XM_034330580.2:229..2217"
                     /db_xref="GeneID:117607197"
ORIGIN      
        1 msaavrertt thtsrkivsh ggpisgqtqp attanslenn ldalledlqt svsrsttpsr
       61 gvrtlspqve yrvaanptkv vsegrpispn rtmttateky vstgpsgass gipglefids
      121 elqnvqpgqs ktiaykqvsy qynknldgkp idswttpdna ltnvssalid dirertyees
      181 tlhsglepvt rtvnrelvys dgstsrskqt splpgtqhkd vkyihessgy qtepvqntlt
      241 tvhasaqeyg nveyvpvpsp tpaipinlap gpntkvttti ktytyelpga petylpgsnv
      301 pgtvvlpgdq titytipkgs tstsdkmvsy qtvtenvppr tptrysspsp vtditkkstt
      361 ihqeakffre ehhgsppgqp iitgyrptsp pletktrvtr nekyyqidgt ypnghppgdr
      421 pdhpsgpviy qnqppiinet htsinrieeh ypsrspssgr yptpdkpgtp vnyyttspqt
      481 tppqssttvy kfsnttttip pnknaedhev llpkpfptgg vqvcppkpvn keqnppkkle
      541 elmasfsdte revlddiekr ekgqrkespa vkkevdfiph tppkvksknv agppvyyppg
      601 saeftkkeea saamsqsgga wqkasgkyey eassksksks ssggavvpvc lplccalpcv
      661 im
//
LOCUS       XP_034186472             166 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light chain Tctex-type protein 2B [Osmia lignaria lignaria].
ACCESSION   XP_034186472
VERSION     XP_034186472.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330581.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="dynein light chain Tctex-type protein 2B"
                     /calculated_mol_wt=19038
     Region          62..165
                     /region_name="DLC-like_TCTEX1D2"
                     /note="dynein light chain (DLC)-like domain found in
                     Tctex1 domain-containing protein 2 (TCTEX1D2) and similar
                     proteins; cd21459"
                     /db_xref="CDD:412007"
     CDS             1..166
                     /gene="LOC117607198"
                     /coded_by="XM_034330581.2:627..1127"
                     /db_xref="GeneID:117607198"
ORIGIN      
        1 meeenipskd stppesetaa ekeeereeek eeektqvnve vledgndvts qiyqirpqlh
       61 ekfkplsake vihnvlfdql sakiydaeda vqwtkdiadi irekikelsf krykyivnvv
      121 lgeqhgagvk mgtrciwdae adtyaydsfa ndtifcvatv yavyfy
//
LOCUS       XP_034186473             166 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light chain Tctex-type protein 2B [Osmia lignaria lignaria].
ACCESSION   XP_034186473
VERSION     XP_034186473.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330582.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="dynein light chain Tctex-type protein 2B"
                     /calculated_mol_wt=19038
     Region          62..165
                     /region_name="DLC-like_TCTEX1D2"
                     /note="dynein light chain (DLC)-like domain found in
                     Tctex1 domain-containing protein 2 (TCTEX1D2) and similar
                     proteins; cd21459"
                     /db_xref="CDD:412007"
     CDS             1..166
                     /gene="LOC117607198"
                     /coded_by="XM_034330582.2:552..1052"
                     /db_xref="GeneID:117607198"
ORIGIN      
        1 meeenipskd stppesetaa ekeeereeek eeektqvnve vledgndvts qiyqirpqlh
       61 ekfkplsake vihnvlfdql sakiydaeda vqwtkdiadi irekikelsf krykyivnvv
      121 lgeqhgagvk mgtrciwdae adtyaydsfa ndtifcvatv yavyfy
//
LOCUS       XP_034186474             166 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light chain Tctex-type protein 2B [Osmia lignaria lignaria].
ACCESSION   XP_034186474
VERSION     XP_034186474.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330583.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="dynein light chain Tctex-type protein 2B"
                     /calculated_mol_wt=19038
     Region          62..165
                     /region_name="DLC-like_TCTEX1D2"
                     /note="dynein light chain (DLC)-like domain found in
                     Tctex1 domain-containing protein 2 (TCTEX1D2) and similar
                     proteins; cd21459"
                     /db_xref="CDD:412007"
     CDS             1..166
                     /gene="LOC117607198"
                     /coded_by="XM_034330583.2:486..986"
                     /db_xref="GeneID:117607198"
ORIGIN      
        1 meeenipskd stppesetaa ekeeereeek eeektqvnve vledgndvts qiyqirpqlh
       61 ekfkplsake vihnvlfdql sakiydaeda vqwtkdiadi irekikelsf krykyivnvv
      121 lgeqhgagvk mgtrciwdae adtyaydsfa ndtifcvatv yavyfy
//
LOCUS       XP_034186475             166 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light chain Tctex-type protein 2B [Osmia lignaria lignaria].
ACCESSION   XP_034186475
VERSION     XP_034186475.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330584.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="dynein light chain Tctex-type protein 2B"
                     /calculated_mol_wt=19038
     Region          62..165
                     /region_name="DLC-like_TCTEX1D2"
                     /note="dynein light chain (DLC)-like domain found in
                     Tctex1 domain-containing protein 2 (TCTEX1D2) and similar
                     proteins; cd21459"
                     /db_xref="CDD:412007"
     CDS             1..166
                     /gene="LOC117607198"
                     /coded_by="XM_034330584.2:444..944"
                     /db_xref="GeneID:117607198"
ORIGIN      
        1 meeenipskd stppesetaa ekeeereeek eeektqvnve vledgndvts qiyqirpqlh
       61 ekfkplsake vihnvlfdql sakiydaeda vqwtkdiadi irekikelsf krykyivnvv
      121 lgeqhgagvk mgtrciwdae adtyaydsfa ndtifcvatv yavyfy
//
LOCUS       XP_034190720             186 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase Hip14 [Osmia lignaria lignaria].
ACCESSION   XP_034190720
VERSION     XP_034190720.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334829.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..186
                     /product="palmitoyltransferase Hip14"
                     /calculated_mol_wt=20695
     Region          35..61
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(36..37,40..42,44..45,49,52,61,63,65,69..70,73..75,
                     77..78,82,85,94,96,98,103..104,107..109,111..112,116,119,
                     128)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          <37..>136
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          63..94
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          96..128
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          102..182
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          130..161
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..186
                     /gene="LOC143305208"
                     /coded_by="XM_034334829.2:103..663"
                     /db_xref="GeneID:143305208"
ORIGIN      
        1 mehhhgsndr ytccssnnnh dvrqslmeme fergiwyaaq yndldrvkal lkkgtpidae
       61 dfagytalhy aarngnddic kfliengaav nartrcgrat plhraamqgh sqivrlllks
      121 ganpnlqdad gctalhkvlv ttpsipvckl lvprtdltlq nnnkqsvkql tqekcpeilq
      181 fistye
//
LOCUS       XP_034190721             186 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase Hip14 [Osmia lignaria lignaria].
ACCESSION   XP_034190721
VERSION     XP_034190721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334830.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..186
                     /product="palmitoyltransferase Hip14"
                     /calculated_mol_wt=20695
     Region          35..61
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(36..37,40..42,44..45,49,52,61,63,65,69..70,73..75,
                     77..78,82,85,94,96,98,103..104,107..109,111..112,116,119,
                     128)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          <37..>136
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          63..94
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          96..128
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          102..182
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          130..161
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..186
                     /gene="LOC143305208"
                     /coded_by="XM_034334830.2:304..864"
                     /db_xref="GeneID:143305208"
ORIGIN      
        1 mehhhgsndr ytccssnnnh dvrqslmeme fergiwyaaq yndldrvkal lkkgtpidae
       61 dfagytalhy aarngnddic kfliengaav nartrcgrat plhraamqgh sqivrlllks
      121 ganpnlqdad gctalhkvlv ttpsipvckl lvprtdltlq nnnkqsvkql tqekcpeilq
      181 fistye
//
LOCUS       XP_076548086             136 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609006 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548086
VERSION     XP_076548086.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691971.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="uncharacterized protein LOC117609006 isoform X1"
                     /calculated_mol_wt=15096
     CDS             1..136
                     /gene="LOC117609006"
                     /coded_by="XM_076691971.1:231..641"
                     /db_xref="GeneID:117609006"
ORIGIN      
        1 mpyilvrgnl asyghrypwr vlvsglkasd ieqlsrfasg gycddstivy lqhpcvilta
       61 levlgykvva ssstsvkqdy neymwtmrkd fsepepepvl kagpafsiin alaelgykvi
      121 cstgeaeilw tlqrel
//
LOCUS       XP_076548087             112 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609006 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548087
VERSION     XP_076548087.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691972.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..112
                     /product="uncharacterized protein LOC117609006 isoform X2"
                     /calculated_mol_wt=12313
     Region          <30..66
                     /region_name="GFRP"
                     /note="GTP cyclohydrolase I feedback regulatory protein
                     (GFRP); pfam06399"
                     /db_xref="CDD:461897"
     CDS             1..112
                     /gene="LOC117609006"
                     /coded_by="XM_076691972.1:209..547"
                     /db_xref="GeneID:117609006"
ORIGIN      
        1 mitasdieql srfasggycd dstivylqhp cviltalevl gykvvassst svkqdyneym
       61 wtmrkdfsep epepvlkagp afsiinalae lgykvicstg eaeilwtlqr el
//
LOCUS       XP_034190723             109 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609006 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190723
VERSION     XP_034190723.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334832.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..109
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..109
                     /product="uncharacterized protein LOC117609006 isoform X3"
                     /calculated_mol_wt=12105
     Region          <54..90
                     /region_name="GFRP"
                     /note="GTP cyclohydrolase I feedback regulatory protein
                     (GFRP); pfam06399"
                     /db_xref="CDD:461897"
     CDS             1..109
                     /gene="LOC117609006"
                     /coded_by="XM_034334832.2:226..555"
                     /db_xref="GeneID:117609006"
ORIGIN      
        1 mpyilvrgnl asyghrypwr vlvsglkasd ieqlsrfasg gycddstivy lqhpcvilta
       61 levlgykvva ssstsvkqdy neymwtmrkd fsepepepvl kaavkgnev
//
LOCUS       XP_034190724             108 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609006 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190724
VERSION     XP_034190724.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334833.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..108
                     /product="uncharacterized protein LOC117609006 isoform X4"
                     /calculated_mol_wt=12034
     CDS             1..108
                     /gene="LOC117609006"
                     /coded_by="XM_034334833.2:232..558"
                     /db_xref="GeneID:117609006"
ORIGIN      
        1 mpyilvrgnl asyghrypwr vlvsglkasd ieqlsrfasg gycddstivy lqhpcvilta
       61 levlgykvva ssstsvkqdy neymwtmrkd fsepepepvl kavkgnev
//
LOCUS       XP_034190725             102 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609006 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190725
VERSION     XP_034190725.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334834.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..102
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..102
                     /product="uncharacterized protein LOC117609006 isoform X5"
                     /calculated_mol_wt=11464
     Region          <54..90
                     /region_name="GFRP"
                     /note="GTP cyclohydrolase I feedback regulatory protein
                     (GFRP); pfam06399"
                     /db_xref="CDD:461897"
     CDS             1..102
                     /gene="LOC117609006"
                     /coded_by="XM_034334834.2:230..538"
                     /db_xref="GeneID:117609006"
ORIGIN      
        1 mpyilvrgnl asyghrypwr vlvsglkasd ieqlsrfasg gycddstivy lqhpcvilta
       61 levlgykvva ssstsvkqdy neymwtmrkd fsepepepvl kq
//
LOCUS       XP_034182872             846 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605544 [Osmia lignaria lignaria].
ACCESSION   XP_034182872
VERSION     XP_034182872.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326981.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..846
                     /product="uncharacterized protein LOC117605544"
                     /calculated_mol_wt=95905
     Region          600..661
                     /region_name="DnaJ"
                     /note="DnaJ domain; pfam00226"
                     /db_xref="CDD:395170"
     Site            order(628..630,638,641..642,645..646)
                     /site_type="other"
                     /note="HSP70 interaction site [polypeptide binding]"
                     /db_xref="CDD:99751"
     Region          688..779
                     /region_name="Jiv90"
                     /note="Cleavage inducing molecular chaperone; pfam14901"
                     /db_xref="CDD:464360"
     CDS             1..846
                     /gene="LOC117605544"
                     /coded_by="XM_034326981.2:233..2773"
                     /db_xref="GeneID:117605544"
ORIGIN      
        1 maeqqqsppd fkhmsldkii eamtsdless nghyvqfgin pqqtathnwg dysssqtrhy
       61 itsqtphtnp stiqpmtpmg gplymhdmsn yesttdsiyf psqavnhlgi nenndligti
      121 dvgggnyinv iygnasqima eqcqlsnits yrnqniigre yglfndrhit vpselppqnf
      181 ngkqlidnlv gnwvpnqsgt yspfgsspnv tpvpqlnvdv repeglpkns tdsqykkpra
      241 vaevkpmrps ysdvltksap appspltvqs ikpkaesvnk kisnknsknk sksaalkrqn
      301 ssgsddhnsp kiqiprkile knnsnlprrw vsldnlgspi esheintfdr snqfekkkvs
      361 ktpkksekae tlnnskiqnn npksagnilk rpiqinnnln ttntssqtvs pekieknqqv
      421 vnkinkeekk vtkeknskrf qtekaqqikk aqrnrrrenr espvkdlckn lnkyanrwik
      481 iglkifhwll hlvsdvvsms anlmiqlgkc gwyhtilylk yswvymavtf skvrilnavg
      541 kqlddwfgns kfafwrkika kseekeensn wirgglesni alpstgeeam krllackgkd
      601 pysilgvtpt csdddikkyy krqaflvhpd knnqpgaeea fkilvhafdi igeperrqaf
      661 dqtrqveaaw gelsdllsql hrkmeqaant irctncglrh kriptqrpcy aarfcaqcki
      721 rhsaregdiw aesrlmgflw hyyacmegav ydvtdwaacq agnlkhlran thsvqyrivl
      781 gqrppsqttv nnnkkrqqid pntseadfed flnnlynhsk tgtsstsgdq nsfrgeckrr
      841 kakrkk
//
LOCUS       XP_034182874             264 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin thioesterase otubain-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182874
VERSION     XP_034182874.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326983.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..264
                     /product="ubiquitin thioesterase otubain-like isoform X1"
                     /calculated_mol_wt=30359
     Region          39..263
                     /region_name="OTUB1"
                     /note="Ubiquitin Thioesterase Otubain-1; cd22763"
                     /db_xref="CDD:438600"
     Site            order(124..125,127..128,131,162..163,166,170,198..199,
                     202..204,206)
                     /site_type="other"
                     /note="E2 enzyme binding site [polypeptide binding]"
                     /db_xref="CDD:438600"
     Site            order(182,184..189,191,210..211,214,240..244,254)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438600"
     CDS             1..264
                     /gene="LOC117605546"
                     /coded_by="XM_034326983.2:368..1162"
                     /db_xref="GeneID:117605546"
ORIGIN      
        1 meetscreql tentdinqde lilqqqrrie meisesialv gekepiksle reytedevyl
       61 skakalgqky syirrtrpdg ncffrafsfa yleklignke eyekfrelal kskdnlvalg
      121 fpqftvgdfh dtfmdvinkv ggdaessyie lhklfneqgc sdyvvvylrl itsgqlrsea
      181 dfyqhfiege rtvsefcrqe vepmykesdh ihiiamstal gtgvrvrymd rgagtevtah
      241 dfpegatpav hllyrpghyd ilyp
//
LOCUS       XP_076548089             259 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin thioesterase otubain-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548089
VERSION     XP_076548089.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691974.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..259
                     /product="ubiquitin thioesterase otubain-like isoform X2"
                     /calculated_mol_wt=29798
     Region          34..258
                     /region_name="OTUB1"
                     /note="Ubiquitin Thioesterase Otubain-1; cd22763"
                     /db_xref="CDD:438600"
     Site            order(119..120,122..123,126,157..158,161,165,193..194,
                     197..199,201)
                     /site_type="other"
                     /note="E2 enzyme binding site [polypeptide binding]"
                     /db_xref="CDD:438600"
     Site            order(177,179..184,186,205..206,209,235..239,249)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:438600"
     CDS             1..259
                     /gene="LOC117605546"
                     /coded_by="XM_076691974.1:308..1087"
                     /db_xref="GeneID:117605546"
ORIGIN      
        1 mdirllfttd inqdelilqq qrriemeise sialvgekep ikslereyte devylskaka
       61 lgqkysyirr trpdgncffr afsfaylekl ignkeeyekf relalkskdn lvalgfpqft
      121 vgdfhdtfmd vinkvggdae ssyielhklf neqgcsdyvv vylrlitsgq lrseadfyqh
      181 fiegertvse fcrqevepmy kesdhihiia mstalgtgvr vrymdrgagt evtahdfpeg
      241 atpavhllyr pghydilyp
//
LOCUS       XP_034182877             216 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605548 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182877
VERSION     XP_034182877.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..216
                     /product="uncharacterized protein LOC117605548 isoform X1"
                     /calculated_mol_wt=25804
     Region          <84..>123
                     /region_name="TM_Y_CoV_Nsp3_C"
                     /note="C-terminus of coronavirus non-structural protein 3,
                     including transmembrane and Y domains; cl40456"
                     /db_xref="CDD:424087"
     CDS             1..216
                     /gene="LOC117605548"
                     /coded_by="XM_034326986.2:351..1001"
                     /db_xref="GeneID:117605548"
ORIGIN      
        1 mdddvsilaq eikkanklpe kdaaflnklc iqlinpvlpq heielragsh cptqkelkrf
       61 ekiahikkgs ynpeedaiit hnwksfcklh nwncrnvqpf lllrednkty irskkerrkf
      121 vqfladglpn rtlysvyhrf rnlyadrvqr rflpeedemi lnhlennanl dekrkytdla
      181 rvlkrtrasi wrrykilkkk rekevqrnnc ismpcc
//
LOCUS       XP_034182878             200 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605548 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182878
VERSION     XP_034182878.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326987.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..200
                     /product="uncharacterized protein LOC117605548 isoform X2"
                     /calculated_mol_wt=23969
     Region          <68..>107
                     /region_name="TM_Y_CoV_Nsp3_C"
                     /note="C-terminus of coronavirus non-structural protein 3,
                     including transmembrane and Y domains; cl40456"
                     /db_xref="CDD:424087"
     CDS             1..200
                     /gene="LOC117605548"
                     /coded_by="XM_034326987.2:351..953"
                     /db_xref="GeneID:117605548"
ORIGIN      
        1 mdddvsilaq eikkanklpe kdaaheielr agshcptqke lkrfekiahi kkgsynpeed
       61 aiithnwksf cklhnwncrn vqpflllred nktyirskke rrkfvqflad glpnrtlysv
      121 yhrfrnlyad rvqrrflpee demilnhlen nanldekrky tdlarvlkrt rasiwrryki
      181 lkkkrekevq rnncismpcc
//
LOCUS       XP_034182880             127 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase epsilon subunit 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182880
VERSION     XP_034182880.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326989.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..127
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..127
                     /product="DNA polymerase epsilon subunit 4 isoform X1"
                     /calculated_mol_wt=14383
     Region          48..126
                     /region_name="HFD_POLE4-like"
                     /note="histone-fold domain found in DNA polymerase epsilon
                     subunit 4 (POLE4) and similar proteins; cd22929"
                     /db_xref="CDD:467054"
     Site            order(48,55,59,62..63,65..70,72..73,75..77,79..90,
                     102..106,118,121..122,125)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:467054"
     Site            order(62,64,72,83,86..87,90,94,97..98,115..118,120..121,
                     124..125)
                     /site_type="other"
                     /note="catalytic subunit binding site [polypeptide
                     binding]"
                     /db_xref="CDD:467054"
     CDS             1..127
                     /gene="PolE4"
                     /coded_by="XM_034326989.2:68..451"
                     /db_xref="GeneID:117605550"
ORIGIN      
        1 manfenegnv sdrelnesna giggensqds qeevennmrt deeqkeklvr lplgriktii
       61 kmdpevnmin qeavfliaks telfidsltk esykytaqmk kktiqkrdve raidnvdalv
      121 flegmln
//
LOCUS       XP_034182881             106 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase epsilon subunit 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182881
VERSION     XP_034182881.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326990.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="DNA polymerase epsilon subunit 4 isoform X2"
                     /calculated_mol_wt=11944
     Region          48..>90
                     /region_name="HFD_SF"
                     /note="histone fold domain (HFD) superfamily; cl45933"
                     /db_xref="CDD:480273"
     CDS             1..106
                     /gene="PolE4"
                     /coded_by="XM_034326990.2:109..429"
                     /db_xref="GeneID:117605550"
ORIGIN      
        1 manfenegnv sdrelnesna giggensqds qeevennmrt deeqkeklvr lplgriktii
       61 kmdpevnmin qeavfliaks tilelmssna qyyhcsnmmi yiiste
//
LOCUS       XP_034182882             105 aa            linear   INV 03-SEP-2025
DEFINITION  BLOC-1 related complex subunit 7 [Osmia lignaria lignaria].
ACCESSION   XP_034182882
VERSION     XP_034182882.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326991.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="BLOC-1 related complex subunit 7"
                     /calculated_mol_wt=11674
     Region          1..103
                     /region_name="BORCS7"
                     /note="BLOC-1-related complex sub-unit 7; pfam16088"
                     /db_xref="CDD:465012"
     CDS             1..105
                     /gene="BORCS7"
                     /coded_by="XM_034326991.2:574..891"
                     /db_xref="GeneID:117605551"
ORIGIN      
        1 masasstsar slfveskmrl adrvqvnvnn iaslarqiqr gskssemlmh saknfaqqeh
       61 gletaennlk kvalitthle yqmesinrss vileqvteqi ramqr
//
LOCUS       XP_034182883             105 aa            linear   INV 03-SEP-2025
DEFINITION  BLOC-1 related complex subunit 7 [Osmia lignaria lignaria].
ACCESSION   XP_034182883
VERSION     XP_034182883.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326992.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="BLOC-1 related complex subunit 7"
                     /calculated_mol_wt=11674
     Region          1..103
                     /region_name="BORCS7"
                     /note="BLOC-1-related complex sub-unit 7; pfam16088"
                     /db_xref="CDD:465012"
     CDS             1..105
                     /gene="BORCS7"
                     /coded_by="XM_034326992.2:102..419"
                     /db_xref="GeneID:117605551"
ORIGIN      
        1 masasstsar slfveskmrl adrvqvnvnn iaslarqiqr gskssemlmh saknfaqqeh
       61 gletaennlk kvalitthle yqmesinrss vileqvteqi ramqr
//
LOCUS       XP_034186571             857 aa            linear   INV 03-SEP-2025
DEFINITION  oculocerebrorenal syndrome of Lowe isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186571
VERSION     XP_034186571.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330680.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..857
                     /product="oculocerebrorenal syndrome of Lowe isoform X1"
                     /calculated_mol_wt=97741
     Region          22..126
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          213..503
                     /region_name="INPP5c_INPP5B"
                     /note="Catalytic inositol polyphosphate 5-phosphatase
                     (INPP5c) domain of Type II inositol polyphosphate
                     5-phosphatase I, Oculocerebrorenal syndrome of Lowe 1, and
                     related proteins; cd09093"
                     /db_xref="CDD:197327"
     Site            order(221,223,252,255,257,260,322,328..329,349,352..353,
                     393,395,397,448..449,462,464,470,494..495)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:197327"
     Site            order(221,252,349,393,395,470,494..495)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197327"
     Site            order(223,252,494)
                     /site_type="other"
                     /note="Mg binding site [ion binding]"
                     /db_xref="CDD:197327"
     Site            order(252,255,257,260,322,328..329,349,352..353,397,
                     448..449,462,464,494)
                     /site_type="other"
                     /note="putative PI/IP binding site [chemical binding]"
                     /db_xref="CDD:197327"
     Site            order(349,395,495)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197327"
     Region          538..>625
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     Region          639..849
                     /region_name="RhoGAP_OCRL1"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in OCRL1-like proteins.
                     OCRL1 (oculocerebrorenal syndrome of Lowe 1)-like proteins
                     contain two conserved domains: a central inositol
                     polyphosphate 5-phosphatase domain and...; cd04380"
                     /db_xref="CDD:239845"
     Site            order(712,747,751,814,817..818,840)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239845"
     Site            712
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239845"
     CDS             1..857
                     /gene="Ocrl"
                     /coded_by="XM_034330680.2:616..3189"
                     /db_xref="GeneID:117607236"
ORIGIN      
        1 mssaeqsviv qskfvsgetv iiaieasliq gwvkaariva llnkgtthal vilitsrtpp
       61 qvysdltier ilpidqdfkc nintnekqqd tldvylnvts rklhlvfemr pgvetsslvs
      121 eifraievyq ktknsasefl wiqkltgstr nlnsnnnedv qdntdplvdl espvlvvtrr
      181 siasgkspva aresavryqm ackeddytys ktfrvfigtw nvngqppnni tlhdwlsydk
      241 sppdiyavgf qeldltkeaf lfndtpreee wrqvvakslh pdgvyeqvai vrlvgmmlii
      301 yalhshmpyi kdvsvdtvgt gimgkmgnkg gvavscsihn tsicfvnahl aahceeyerr
      361 nqdyadictr lsfskyvppk nfkdhvqiyw lgdlnyrite mdvavakkhi dvenyvpvla
      421 ldqlgqqrrl grvlqgfhea eikfkptyky dpgtdnwdss ekgrapawcd rilwkgeait
      481 sidyrshpel kisdhkpvsa ifdsqiriid iakyrkthee vmkkldklen eflpqvmvdt
      541 teiifdtlkf lepsskelii antgqvpvqf efikklgdts yckdwldiep ykgfikpgek
      601 cdtrfeiyvd krsacklnsg edklydilil hleggkdifi tvtgtyersc fgssmealvh
      661 ipvpireipv grlmelennk nlsqepypvp kevwflvdrl yrhgiktagl fetpglhsei
      721 iairdwldnw sqdpmpgsvh svaealllll estaeplipy nlhtvclntv tnylqckqiv
      781 mqlpeirrtv fvyicyflqe llnhtqdnel daktlatlfg svflrdpprs rgdrdrrkal
      841 fvyhflvndq sdfilgr
//
LOCUS       XP_034186570             857 aa            linear   INV 03-SEP-2025
DEFINITION  oculocerebrorenal syndrome of Lowe isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186570
VERSION     XP_034186570.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330679.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..857
                     /product="oculocerebrorenal syndrome of Lowe isoform X1"
                     /calculated_mol_wt=97741
     Region          22..126
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          213..503
                     /region_name="INPP5c_INPP5B"
                     /note="Catalytic inositol polyphosphate 5-phosphatase
                     (INPP5c) domain of Type II inositol polyphosphate
                     5-phosphatase I, Oculocerebrorenal syndrome of Lowe 1, and
                     related proteins; cd09093"
                     /db_xref="CDD:197327"
     Site            order(221,223,252,255,257,260,322,328..329,349,352..353,
                     393,395,397,448..449,462,464,470,494..495)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:197327"
     Site            order(221,252,349,393,395,470,494..495)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197327"
     Site            order(223,252,494)
                     /site_type="other"
                     /note="Mg binding site [ion binding]"
                     /db_xref="CDD:197327"
     Site            order(252,255,257,260,322,328..329,349,352..353,397,
                     448..449,462,464,494)
                     /site_type="other"
                     /note="putative PI/IP binding site [chemical binding]"
                     /db_xref="CDD:197327"
     Site            order(349,395,495)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197327"
     Region          538..>625
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     Region          639..849
                     /region_name="RhoGAP_OCRL1"
                     /note="RhoGAP (GTPase-activator protein [GAP] for Rho-like
                     small GTPases) domain present in OCRL1-like proteins.
                     OCRL1 (oculocerebrorenal syndrome of Lowe 1)-like proteins
                     contain two conserved domains: a central inositol
                     polyphosphate 5-phosphatase domain and...; cd04380"
                     /db_xref="CDD:239845"
     Site            order(712,747,751,814,817..818,840)
                     /site_type="other"
                     /note="putative GTPase interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:239845"
     Site            712
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239845"
     CDS             1..857
                     /gene="Ocrl"
                     /coded_by="XM_034330679.2:483..3056"
                     /db_xref="GeneID:117607236"
ORIGIN      
        1 mssaeqsviv qskfvsgetv iiaieasliq gwvkaariva llnkgtthal vilitsrtpp
       61 qvysdltier ilpidqdfkc nintnekqqd tldvylnvts rklhlvfemr pgvetsslvs
      121 eifraievyq ktknsasefl wiqkltgstr nlnsnnnedv qdntdplvdl espvlvvtrr
      181 siasgkspva aresavryqm ackeddytys ktfrvfigtw nvngqppnni tlhdwlsydk
      241 sppdiyavgf qeldltkeaf lfndtpreee wrqvvakslh pdgvyeqvai vrlvgmmlii
      301 yalhshmpyi kdvsvdtvgt gimgkmgnkg gvavscsihn tsicfvnahl aahceeyerr
      361 nqdyadictr lsfskyvppk nfkdhvqiyw lgdlnyrite mdvavakkhi dvenyvpvla
      421 ldqlgqqrrl grvlqgfhea eikfkptyky dpgtdnwdss ekgrapawcd rilwkgeait
      481 sidyrshpel kisdhkpvsa ifdsqiriid iakyrkthee vmkkldklen eflpqvmvdt
      541 teiifdtlkf lepsskelii antgqvpvqf efikklgdts yckdwldiep ykgfikpgek
      601 cdtrfeiyvd krsacklnsg edklydilil hleggkdifi tvtgtyersc fgssmealvh
      661 ipvpireipv grlmelennk nlsqepypvp kevwflvdrl yrhgiktagl fetpglhsei
      721 iairdwldnw sqdpmpgsvh svaealllll estaeplipy nlhtvclntv tnylqckqiv
      781 mqlpeirrtv fvyicyflqe llnhtqdnel daktlatlfg svflrdpprs rgdrdrrkal
      841 fvyhflvndq sdfilgr
//
LOCUS       XP_076548091             687 aa            linear   INV 03-SEP-2025
DEFINITION  oculocerebrorenal syndrome of Lowe isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548091
VERSION     XP_076548091.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691976.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..687
                     /product="oculocerebrorenal syndrome of Lowe isoform X2"
                     /calculated_mol_wt=78026
     Region          22..126
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          213..503
                     /region_name="INPP5c_INPP5B"
                     /note="Catalytic inositol polyphosphate 5-phosphatase
                     (INPP5c) domain of Type II inositol polyphosphate
                     5-phosphatase I, Oculocerebrorenal syndrome of Lowe 1, and
                     related proteins; cd09093"
                     /db_xref="CDD:197327"
     Site            order(221,223,252,255,257,260,322,328..329,349,352..353,
                     393,395,397,448..449,462,464,470,494..495)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:197327"
     Site            order(221,252,349,393,395,470,494..495)
                     /site_type="active"
                     /note="putative catalytic site [active]"
                     /db_xref="CDD:197327"
     Site            order(223,252,494)
                     /site_type="other"
                     /note="Mg binding site [ion binding]"
                     /db_xref="CDD:197327"
     Site            order(252,255,257,260,322,328..329,349,352..353,397,
                     448..449,462,464,494)
                     /site_type="other"
                     /note="putative PI/IP binding site [chemical binding]"
                     /db_xref="CDD:197327"
     Site            order(349,395,495)
                     /site_type="other"
                     /note="putative phosphate binding site [ion binding]"
                     /db_xref="CDD:197327"
     Region          538..>625
                     /region_name="Motile_Sperm"
                     /note="MSP (Major sperm protein) domain; pfam00635"
                     /db_xref="CDD:459882"
     Region          639..>677
                     /region_name="RhoGAP"
                     /note="GTPase-activator protein (GAP) for Rho-like
                     GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases.
                     Small GTPases (G proteins) cluster into distinct families,
                     and all act as molecular switches, active in their
                     GTP-bound form but inactive when bound to...; cl02570"
                     /db_xref="CDD:470621"
     CDS             1..687
                     /gene="Ocrl"
                     /coded_by="XM_076691976.1:617..2680"
                     /db_xref="GeneID:117607236"
ORIGIN      
        1 mssaeqsviv qskfvsgetv iiaieasliq gwvkaariva llnkgtthal vilitsrtpp
       61 qvysdltier ilpidqdfkc nintnekqqd tldvylnvts rklhlvfemr pgvetsslvs
      121 eifraievyq ktknsasefl wiqkltgstr nlnsnnnedv qdntdplvdl espvlvvtrr
      181 siasgkspva aresavryqm ackeddytys ktfrvfigtw nvngqppnni tlhdwlsydk
      241 sppdiyavgf qeldltkeaf lfndtpreee wrqvvakslh pdgvyeqvai vrlvgmmlii
      301 yalhshmpyi kdvsvdtvgt gimgkmgnkg gvavscsihn tsicfvnahl aahceeyerr
      361 nqdyadictr lsfskyvppk nfkdhvqiyw lgdlnyrite mdvavakkhi dvenyvpvla
      421 ldqlgqqrrl grvlqgfhea eikfkptyky dpgtdnwdss ekgrapawcd rilwkgeait
      481 sidyrshpel kisdhkpvsa ifdsqiriid iakyrkthee vmkkldklen eflpqvmvdt
      541 teiifdtlkf lepsskelii antgqvpvqf efikklgdts yckdwldiep ykgfikpgek
      601 cdtrfeiyvd krsacklnsg edklydilil hleggkdifi tvtgtyersc fgssmealvh
      661 ipvpireipv grlmelnltl fqkkygs
//
LOCUS       XP_034186573             520 aa            linear   INV 03-SEP-2025
DEFINITION  karyopherin subunit alpha [Osmia lignaria lignaria].
ACCESSION   XP_034186573
VERSION     XP_034186573.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330682.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..520
                     /product="karyopherin subunit alpha"
                     /calculated_mol_wt=56817
     Region          16..519
                     /region_name="SRP1"
                     /note="Karyopherin (importin) alpha [Intracellular
                     trafficking and secretion]; COG5064"
                     /db_xref="CDD:227396"
     Region          109..141
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(132,136,140,174,178,182,217,221,225)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          149..185
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          191..226
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          243..268
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Site            order(259,263,267,301,305,309,343,347,351)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293788"
     Region          276..312
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          318..352
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          360..396
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     Region          402..437
                     /region_name="armadillo repeat"
                     /note="armadillo repeat [structural motif]"
                     /db_xref="CDD:293788"
     CDS             1..520
                     /gene="Pen"
                     /coded_by="XM_034330682.2:192..1754"
                     /db_xref="GeneID:117607237"
ORIGIN      
        1 mpaseadchs rkavfkfnnk heearrrrne isvelrkark deqllkrrnl nigkepqtpa
       61 ednnlspltt siddifdgmk ssdetvllqa tqacrkmlsr eknppidsmi krgivpkcve
      121 fldchhnaal qfeaawaltn vasgtseqth vvvkhgaipk laallksasp nvaeqavwal
      181 gniagdgpma rdlvlaydtm slllelikpd tsvtftrniv wtlsnlcrnk nppppfeivr
      241 talpvlnrll sspdkhilad acwalsyltd gsndkiqavv esgvipklve llsspevtvl
      301 tpalravgni vtgndmqtda iisagglqhl glllqhhrin ivkeaawtis nvtagnmeqi
      361 qhvinagllp plihvlqsgd fksqkeaawa vtnltsggsv qqlaqlvqlg vlapfcklle
      421 akewktvlvv ldgltnilna aekmgevdrv almieevggv dkledlqhhe veevyqkaia
      481 midtffsdgd aeesvltsaa ttdgqlefkp tedtqkdfnf
//
LOCUS       XP_034186575             298 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase, gamma subunit [Osmia lignaria lignaria].
ACCESSION   XP_034186575
VERSION     XP_034186575.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330684.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..298
                     /product="ATP synthase, gamma subunit"
                     /calculated_mol_wt=32713
     Region          26..294
                     /region_name="F1-ATPase_gamma"
                     /note="mitochondrial ATP synthase gamma subunit; cd12151"
                     /db_xref="CDD:213394"
     Site            order(29,34,40,44,47..48,50..51,54..55,100,102..105,
                     135..136,139..140,143,260,263..264,267,273,276..277,
                     279..280,283..284,287,289..294)
                     /site_type="other"
                     /note="core domain interface [polypeptide binding]"
                     /db_xref="CDD:213394"
     Site            order(65..66,68..69,72,75..76,225,228..229,232)
                     /site_type="other"
                     /note="delta subunit interface [polypeptide binding]"
                     /db_xref="CDD:213394"
     Site            order(138,150..155,228,231..232)
                     /site_type="other"
                     /note="epsilon subunit interface [polypeptide binding]"
                     /db_xref="CDD:213394"
     CDS             1..298
                     /gene="ATPsyngamma"
                     /coded_by="XM_034330684.2:157..1053"
                     /db_xref="GeneID:117607238"
ORIGIN      
        1 mfsnrvttil rlaaqqqqqq qqrgmatlka isirlksvkn iqkitqsmkm vsaakysrae
       61 rdlkqarplg vgtkmfyeqa eiqappeepk klviavtsdr glcgavhtgv srnirdalla
      121 danerentki vcigekskai lsrifannil fvasevgrkp ptfndaakva ievlnsgytf
      181 gsgrivynkf ksvvsyavdq lplfdknava tapklsvyds ldenvvqsyl efslasllyy
      241 smkegacseq ssrmtamdna sknagemidk ltltfnrtrq avitreliei isgaaale
//
LOCUS       XP_034183252             783 aa            linear   INV 03-SEP-2025
DEFINITION  putative ATP-dependent RNA helicase DDX10 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183252
VERSION     XP_034183252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327361.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..783
                     /product="putative ATP-dependent RNA helicase DDX10"
                     /calculated_mol_wt=90210
     Region          51..248
                     /region_name="DEADc_DDX10"
                     /note="DEAD-box helicase domain of DEAD box protein 10;
                     cd17941"
                     /db_xref="CDD:350699"
     Site            order(60,62,64,67,85..92,194)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350699"
     Site            193..196
                     /site_type="other"
                     /note="DEAD box helicase motif"
                     /db_xref="CDD:350699"
     Region          259..390
                     /region_name="SF2_C_DEAD"
                     /note="C-terminal helicase domain of the DEAD box
                     helicases; cd18787"
                     /db_xref="CDD:350174"
     Site            order(349,351,376,379..380)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:350174"
     Region          422..480
                     /region_name="DUF4217"
                     /note="Domain of unknown function (DUF4217); pfam13959"
                     /db_xref="CDD:464056"
     Region          576..>740
                     /region_name="PRK05901"
                     /note="RNA polymerase sigma factor; Provisional"
                     /db_xref="CDD:235640"
     CDS             1..783
                     /gene="LOC117605717"
                     /coded_by="XM_034327361.2:43..2394"
                     /db_xref="GeneID:117605717"
ORIGIN      
        1 mfkkkkvkky yrtekkqlpe skiiaelqsr ynsidetkit kfkelplsqk tlkgltenny
       61 iemtdiqrqs iglalrgsdi lgaaktgsgk tlaflipvie ilyckqwtrl dglgaliitp
      121 trelayqiye tlrkvgqyhd vsagliiggk dlkfekkrmd qcniiictpg rllqhmdenp
      181 lfdcinmqvl vldeadrcld mgfeqtmnsi idnlpakrqt llfsatqtks vkdlarlslk
      241 dpmyvsvheh athttpealq qsyvicalen kvsmiwsfik nhlkqkiivf fssckqvkyi
      301 yevlcrlrpg isllalygtl hqlrrmeiye tfckkqfavl fatdiaargl dfpavnwvvq
      361 mdcpedvnay ihragrtarf qsggesllvl lpseekmvek lkerkipism irinpnklqs
      421 pqrkiealla rdvtlkesaq rsfiayvrsv flmkdkevfn vralntdlfa rslglaippr
      481 irflqrmeqk qqlsiknain dqiirnhelg nntkevdsny sdndheeheq ketsrltnas
      541 dfafadsddd diltvkrkni niddipnivn ddedensnkk kvvtkaavak kilrkkiipn
      601 kkitfdeegk elldsakmki selakqyene vdsginieia kqilreedrf dkqrfkekvk
      661 ekhreekrkl kaskkkvene sndedemeen adddnasdse ysapdlswlp dpdkiygkqk
      721 sdekssdtae sveneeeski hrlpkrklmt qkeykkkkqk lslkientnl ktdeelvlql
      781 lqn
//
LOCUS       XP_034183025             498 aa            linear   INV 03-SEP-2025
DEFINITION  EF-hand domain-containing family member B [Osmia lignaria
            lignaria].
ACCESSION   XP_034183025
VERSION     XP_034183025.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327134.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="EF-hand domain-containing family member B"
                     /calculated_mol_wt=58162
     CDS             1..498
                     /gene="LOC117605609"
                     /coded_by="XM_034327134.2:124..1620"
                     /db_xref="GeneID:117605609"
ORIGIN      
        1 makycvknrk elldeinenr nsvnkkskhg etstynikec feeysledkv etlksilshn
       61 gklkqnepsn innilhikkp igvkeclqan qetpfqtlif elkntvmnsy wnkevgktcy
      121 qvpnlpvgmn plettfgkri epgetvakil hekmnknnil ekdivemykk shnsylpaeq
      181 ikrhykspfn enlyfgkpin irakgtgvkk llkwidvdth tiidpkfsdf kehsnveevs
      241 nlnntpvctn mehdtnkrtd keeirnvfne cslndetikq ynylqyinnl rlklkkripe
      301 iqfvdifedl lyfdkdyiei lpadkifpil akykiyvnev llmplldllq irkgktvnyr
      361 allnllnwry dfptlpkiek lpldcqyynt tynetignlq einttsisaa gissknmedn
      421 kttayslifp niftryglsq ldlskprske evksifenie iqfpnnsfdl lweeglkksg
      481 ennlsieafn nlfdqyny
//
LOCUS       XP_034183246             384 aa            linear   INV 03-SEP-2025
DEFINITION  isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma
            [Osmia lignaria lignaria].
ACCESSION   XP_034183246
VERSION     XP_034183246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327355.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="isocitrate dehydrogenase (NAD(+)) 3
                     non-catalytic subunit gamma"
                     /calculated_mol_wt=42008
     Region          49..379
                     /region_name="Iso_dh"
                     /note="Isocitrate/isopropylmalate dehydrogenase; cl00445"
                     /db_xref="CDD:444908"
     CDS             1..384
                     /gene="Idh3g"
                     /coded_by="XM_034327355.2:132..1286"
                     /db_xref="GeneID:143305211"
ORIGIN      
        1 matrsmvkyl raksvvsqih rcaslsafei qhktptikkv mpipkahygg rhtvtllpga
       61 gigpelmtyv kevfsyagvp vdfedvdidp naddnvdldy aitsirrngv alkgnietrs
      121 teagvlsrnv alrnqldlyv nvlhcvsypg vnsrhknidi vivrqntege yamlehesvk
      181 gvvesmkvit stnserlary afeyakrngr kkittvhkan imklsdglfl etsrkvakdy
      241 peitlndmii dntcmqlvsn phqfdivltt nlygaivsnv vcgllggagl lagknfgdhy
      301 tvfepgtrnt gtgiagknva npiamlnaav dmlrhlghkh hailiqnain ktinqgihtr
      361 dlggqatsre vvdaimkhik iast
//
LOCUS       XP_034183248             384 aa            linear   INV 03-SEP-2025
DEFINITION  isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma
            [Osmia lignaria lignaria].
ACCESSION   XP_034183248
VERSION     XP_034183248.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327357.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="isocitrate dehydrogenase (NAD(+)) 3
                     non-catalytic subunit gamma"
                     /calculated_mol_wt=42008
     Region          49..379
                     /region_name="Iso_dh"
                     /note="Isocitrate/isopropylmalate dehydrogenase; cl00445"
                     /db_xref="CDD:444908"
     CDS             1..384
                     /gene="Idh3g"
                     /coded_by="XM_034327357.2:151..1305"
                     /db_xref="GeneID:143305211"
ORIGIN      
        1 matrsmvkyl raksvvsqih rcaslsafei qhktptikkv mpipkahygg rhtvtllpga
       61 gigpelmtyv kevfsyagvp vdfedvdidp naddnvdldy aitsirrngv alkgnietrs
      121 teagvlsrnv alrnqldlyv nvlhcvsypg vnsrhknidi vivrqntege yamlehesvk
      181 gvvesmkvit stnserlary afeyakrngr kkittvhkan imklsdglfl etsrkvakdy
      241 peitlndmii dntcmqlvsn phqfdivltt nlygaivsnv vcgllggagl lagknfgdhy
      301 tvfepgtrnt gtgiagknva npiamlnaav dmlrhlghkh hailiqnain ktinqgihtr
      361 dlggqatsre vvdaimkhik iast
//
LOCUS       XP_034183249             384 aa            linear   INV 03-SEP-2025
DEFINITION  isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma
            [Osmia lignaria lignaria].
ACCESSION   XP_034183249
VERSION     XP_034183249.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327358.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="isocitrate dehydrogenase (NAD(+)) 3
                     non-catalytic subunit gamma"
                     /calculated_mol_wt=42008
     Region          49..379
                     /region_name="Iso_dh"
                     /note="Isocitrate/isopropylmalate dehydrogenase; cl00445"
                     /db_xref="CDD:444908"
     CDS             1..384
                     /gene="Idh3g"
                     /coded_by="XM_034327358.2:95..1249"
                     /db_xref="GeneID:143305211"
ORIGIN      
        1 matrsmvkyl raksvvsqih rcaslsafei qhktptikkv mpipkahygg rhtvtllpga
       61 gigpelmtyv kevfsyagvp vdfedvdidp naddnvdldy aitsirrngv alkgnietrs
      121 teagvlsrnv alrnqldlyv nvlhcvsypg vnsrhknidi vivrqntege yamlehesvk
      181 gvvesmkvit stnserlary afeyakrngr kkittvhkan imklsdglfl etsrkvakdy
      241 peitlndmii dntcmqlvsn phqfdivltt nlygaivsnv vcgllggagl lagknfgdhy
      301 tvfepgtrnt gtgiagknva npiamlnaav dmlrhlghkh hailiqnain ktinqgihtr
      361 dlggqatsre vvdaimkhik iast
//
LOCUS       XP_034183250             384 aa            linear   INV 03-SEP-2025
DEFINITION  isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma
            [Osmia lignaria lignaria].
ACCESSION   XP_034183250
VERSION     XP_034183250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327359.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..384
                     /product="isocitrate dehydrogenase (NAD(+)) 3
                     non-catalytic subunit gamma"
                     /calculated_mol_wt=42008
     Region          49..379
                     /region_name="Iso_dh"
                     /note="Isocitrate/isopropylmalate dehydrogenase; cl00445"
                     /db_xref="CDD:444908"
     CDS             1..384
                     /gene="Idh3g"
                     /coded_by="XM_034327359.2:79..1233"
                     /db_xref="GeneID:143305211"
ORIGIN      
        1 matrsmvkyl raksvvsqih rcaslsafei qhktptikkv mpipkahygg rhtvtllpga
       61 gigpelmtyv kevfsyagvp vdfedvdidp naddnvdldy aitsirrngv alkgnietrs
      121 teagvlsrnv alrnqldlyv nvlhcvsypg vnsrhknidi vivrqntege yamlehesvk
      181 gvvesmkvit stnserlary afeyakrngr kkittvhkan imklsdglfl etsrkvakdy
      241 peitlndmii dntcmqlvsn phqfdivltt nlygaivsnv vcgllggagl lagknfgdhy
      301 tvfepgtrnt gtgiagknva npiamlnaav dmlrhlghkh hailiqnain ktinqgihtr
      361 dlggqatsre vvdaimkhik iast
//
LOCUS       XP_034183026             175 aa            linear   INV 03-SEP-2025
DEFINITION  ER membrane protein complex subunit 4 [Osmia lignaria lignaria].
ACCESSION   XP_034183026
VERSION     XP_034183026.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327135.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..175
                     /product="ER membrane protein complex subunit 4"
                     /calculated_mol_wt=19447
     Region          49..161
                     /region_name="DUF1077"
                     /note="Protein of unknown function (DUF1077); pfam06417"
                     /db_xref="CDD:461902"
     CDS             1..175
                     /gene="EMC4"
                     /coded_by="XM_034327135.2:142..669"
                     /db_xref="GeneID:117605610"
ORIGIN      
        1 mtavkqntkr skwaldfahk nkqeknvdia sppgytpava lfhaadsire tdsnhliikk
       61 swdlalgplk qvpmnlfimy magnsisifp immvgmliir pvkalfvlqq tfkviegtha
      121 fgqkfvyflg qlvnialaly kcqsmgllpt hasdwlafve pqarleysgg gfiyv
//
LOCUS       XP_034183261             147 aa            linear   INV 03-SEP-2025
DEFINITION  M-phase phosphoprotein 6 [Osmia lignaria lignaria].
ACCESSION   XP_034183261
VERSION     XP_034183261.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327370.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..147
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..147
                     /product="M-phase phosphoprotein 6"
                     /calculated_mol_wt=17486
     Region          7..112
                     /region_name="MPP6"
                     /note="M-phase phosphoprotein 6; pfam10175"
                     /db_xref="CDD:462978"
     CDS             1..147
                     /gene="Mpp6"
                     /coded_by="XM_034327370.2:94..537"
                     /db_xref="GeneID:117605723"
ORIGIN      
        1 mntnkaklsk silemkfmkr tkekiekeqf qeegeeyfgn eltkrmkqes erfiiepsyt
       61 fcekliegrv sfqgmnlkie klmeekenhk tleetsketd isdaqmtqdw kgykkkitte
      121 hnyklskesn nefepvvkkp kflkpqd
//
LOCUS       XP_034183028              60 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605612 [Osmia lignaria lignaria].
ACCESSION   XP_034183028
VERSION     XP_034183028.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327137.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..60
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..60
                     /product="uncharacterized protein LOC117605612"
                     /calculated_mol_wt=6924
     Region          18..60
                     /region_name="DUF4535"
                     /note="Domain of unknown function (DUF4535); pfam15054"
                     /db_xref="CDD:464473"
     CDS             1..60
                     /gene="LOC117605612"
                     /coded_by="XM_034327137.2:104..286"
                     /db_xref="GeneID:117605612"
ORIGIN      
        1 mkrvaarlht sfhtlycmlt fsvgvyagiy isqnyevprv depakiieri kkfadeykkk
//
LOCUS       XP_034182967            1170 aa            linear   INV 03-SEP-2025
DEFINITION  endoplasmic reticulum transmembrane helix translocase [Osmia
            lignaria lignaria].
ACCESSION   XP_034182967
VERSION     XP_034182967.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327076.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182967.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1170
                     /product="endoplasmic reticulum transmembrane helix
                     translocase"
                     /calculated_mol_wt=130931
     Region          166..1101
                     /region_name="P-type_ATPase_cation"
                     /note="P-type cation-transporting ATPases, similar to
                     human cation-transporting ATPase type 13A1 (ATP13A1) and
                     Saccharomyces manganese-transporting ATPase 1 Spf1p;
                     cd07543"
                     /db_xref="CDD:319843"
     Site            order(485..487,577,605..607,637,699..701,792,795,798,817,
                     820)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:319843"
     Site            order(935,939..940,942..943,970,973..974)
                     /site_type="other"
                     /note="putative cation binding site [ion binding]"
                     /db_xref="CDD:319843"
     CDS             1..1170
                     /gene="LOC117605577"
                     /coded_by="XM_034327076.2:221..3733"
                     /db_xref="GeneID:117605577"
ORIGIN      
        1 mattassrvd elvqtvtlhn prkllftgyi lpsvilhaaw iyswifiygi deyydaglvg
       61 iaaigvlqif iclccqwsvh ihtflncsse kdpykakiak viptpnngss elvklhhsne
      121 qepwfifqkt kylwnpnkkc feglqypinh svkhycewkg yldekdiaia eekysknkld
      181 mvvpefkelf keraiapffv fqvfcvalwc ldkywyysif tlimlimfec tlvqqqlrnm
      241 aeirkmgnkp ymimvyrnrr wrsmftdqll pgdivsitrs qndnlvpcdm lllrgpcvvd
      301 esmltgesvp qmkepiedid gnrqldiegs dklhvlfggt kvvqhtppsk nlsglkatdn
      361 gcvayvlrtg fstsqgkllr tilfgvkrvt annletfgfi lfllvfaiaa atyvwikgse
      421 dptrnryklf lectliltsv vppelpiels lavntsllal sklgvfctep fripfagkve
      481 iccfdktgtl tsdnlivegl agiegkadvm qlsdaplesi qvlatchslv qlddeivgdp
      541 lekatlkain wsltkndsmi prkgqspvlk ivqrhhfssa lkrmsvvasy tmpgsseiny
      601 mttvkgapei ikdmlssvpe nydstylsls rrgarvlalg yrklpgplss qdlreltrdr
      661 leknltfagf viiscplkpd skavikeivn ashsvvmitg dnpltachvs relhftkkpv
      721 tlilttydge wlwesvdkki nlplnktdvt srgneiwrky alcltgeglt ylnenehell
      781 rkllphivif arcepkqkef iivqlqnlgy ttlmcgdgtn dvgalkhaqv gvailssppv
      841 lsertfsdkq dsmktenmis nsmvngsrmn trgsantrvr lqkvlkelee qsvivklgda
      901 siaapftskm ssiqcichvi kqgrctlvtt lqmfkilaln alalaysqsv lyldgikfsd
      961 aqatlqgvll atcflfisrs kplktlskqr plpnifnlyt iatvllqfav hffslvylvr
     1021 eatllspksd klaailapsn asnlnvtata ssnfgdeseq fepnllnstv yiiamalqls
     1081 tfainyrgqp ymeslvqnks llyslicsaa vilglacgfl pelaaqfeiv dfpsdfrkll
     1141 vqvlvadfvl ayvvdrvclw lfgegrhqkl
//
LOCUS       XP_034182969             598 aa            linear   INV 03-SEP-2025
DEFINITION  sodium-coupled monocarboxylate transporter 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182969
VERSION     XP_034182969.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327078.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182969.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..598
                     /product="sodium-coupled monocarboxylate transporter 1"
                     /calculated_mol_wt=65178
     Region          13..530
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(62,65,348,351..352)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..598
                     /gene="LOC117605578"
                     /coded_by="XM_034327078.2:738..2534"
                     /db_xref="GeneID:117605578"
ORIGIN      
        1 mleieergyf hwadwlvfal mlavsaaagl whyrraqkss tedyllggks mslfpvsasl
       61 vasfisgvti lgtpaeiynf gtqywitiis iffsgvvvat vyapvfvslg lnsvyeylei
      121 rfnrgvrtli slifvidvvl yqsivvyvpa lalnqvsgid vhligiivcl vcvfytvlgg
      181 iravvwtdal qvavmvagml tvtvlgtyri gaaeiwkraq dvnrieflnf dpspytrhtv
      241 wtvligswly stayiavnqt mvqryrslkd lktsklslai ftisimlfis lccwcglvli
      301 awwsppkcdp rasglitadd qllpayvmei agrlhgvpgl fvagifgaal stlsvgfnst
      361 svvlledfvk gcfgmkltdr cstifvktvv vllgsialgl ifmveklggv laitgslaai
      421 aagtsfgvft lgilfpwtns kgafmgaivg fviagwaslg anwsigagll vpkklpvpls
      481 qcsgnisesf lkqfdrpned dvfplyrlsy hwftglgtli vivvgnfisw wtgptdpssi
      541 dkrllsplih swlptpkdqn gttenstgip alqttanmll adlkekrrre nfpngvti
//
LOCUS       XP_034182970             598 aa            linear   INV 03-SEP-2025
DEFINITION  sodium-coupled monocarboxylate transporter 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182970
VERSION     XP_034182970.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327079.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182970.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..598
                     /product="sodium-coupled monocarboxylate transporter 1"
                     /calculated_mol_wt=65178
     Region          13..530
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(62,65,348,351..352)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..598
                     /gene="LOC117605578"
                     /coded_by="XM_034327079.2:380..2176"
                     /db_xref="GeneID:117605578"
ORIGIN      
        1 mleieergyf hwadwlvfal mlavsaaagl whyrraqkss tedyllggks mslfpvsasl
       61 vasfisgvti lgtpaeiynf gtqywitiis iffsgvvvat vyapvfvslg lnsvyeylei
      121 rfnrgvrtli slifvidvvl yqsivvyvpa lalnqvsgid vhligiivcl vcvfytvlgg
      181 iravvwtdal qvavmvagml tvtvlgtyri gaaeiwkraq dvnrieflnf dpspytrhtv
      241 wtvligswly stayiavnqt mvqryrslkd lktsklslai ftisimlfis lccwcglvli
      301 awwsppkcdp rasglitadd qllpayvmei agrlhgvpgl fvagifgaal stlsvgfnst
      361 svvlledfvk gcfgmkltdr cstifvktvv vllgsialgl ifmveklggv laitgslaai
      421 aagtsfgvft lgilfpwtns kgafmgaivg fviagwaslg anwsigagll vpkklpvpls
      481 qcsgnisesf lkqfdrpned dvfplyrlsy hwftglgtli vivvgnfisw wtgptdpssi
      541 dkrllsplih swlptpkdqn gttenstgip alqttanmll adlkekrrre nfpngvti
//
LOCUS       XP_034183020            1773 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605606 [Osmia lignaria lignaria].
ACCESSION   XP_034183020
VERSION     XP_034183020.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327129.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183020.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1773
                     /product="uncharacterized protein LOC117605606"
                     /calculated_mol_wt=180636
     Region          65..228
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     Region          496..575
                     /region_name="Collagen"
                     /note="Collagen triple helix repeat (20 copies);
                     pfam01391"
                     /db_xref="CDD:460189"
     Region          <550..>837
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <739..>959
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <868..>1099
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1163..>1445
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1426..1476
                     /region_name="Collagen"
                     /note="Collagen triple helix repeat (20 copies);
                     pfam01391"
                     /db_xref="CDD:460189"
     Region          1551..1772
                     /region_name="COLFI"
                     /note="Fibrillar collagen C-terminal domain; cl02436"
                     /db_xref="CDD:470578"
     CDS             1..1773
                     /gene="LOC117605606"
                     /coded_by="XM_034327129.2:163..5484"
                     /db_xref="GeneID:117605606"
ORIGIN      
        1 mrrdhvkqqw rritvgsvsw tlcflllgly pvladitlqd meknivdlvd gtgmqqepvg
       61 vmlaqnrchr ndtvaynite salltintnt lfptgiprdf silvvarpki gypvatlfai
      121 ysdsgeeqlv lslgndvtly ysttpddedq sisfgvdisd grwhrlgisi kgdtvtlivd
      181 ctktiskelr rkldetisvd giiiigqqli dgnmylgsle mlkiapipda ayeictifap
      241 dcrnqsryrs rinsypsqvr nygpvirtts ssyyeastni gitqpsqnfr ssseesaynr
      301 sygtnynnin fsrnhdrtvy edyeeenkeg svednegynl tykvnttsrp dfqetlsnsi
      361 ytidqyqttt petpdssenl iggiqdtlky tgssegsyys avyyntrgpp gprgfpgppg
      421 vpgppgkkge pgrdglgglq gvpgppgnvf vlptlnqqgn ekgpdtqvea lrqmlsqhmm
      481 amrgaegplg ltgisgpegp sgpqgqkgep gevgepgprg ergipgntgr egrrgrpgrd
      541 gerglsgppg mkgeqgvpgl pglpgdkger gppgatgepg lpghegmqgd dgppglpglp
      601 gemgprgfig prgfpglpgn pgipgsegpp gakgntgppg ssgspgspgp agpagppgpq
      661 gllgptglvg pqgkpgipgl pgaegppghp gnpgapgikg eqgpqgpqgp lgfpgvrgvk
      721 gdegkrgtpg ergdkgergl egekgdsgak gepgssgpqg ipgleglegs kgfegprger
      781 gptgplgekg ktgppgfvgy pgnigekgdk gqpgregptg dkgergnnga pgergepgpr
      841 gfrgargrrg aeglpgpkgd tgqpgspglq gepgppgveg prgfvgppgq agldgkdgia
      901 gphgergpvg dsgpigppga sgvigpqgpa gepgqpgepg npgnpglpge tgppgeqgke
      961 gppgpsglpg kdgppgpsgl pgfpgergls glpgmlgskg eigptgppgp pgdkgaqgea
     1021 gkdgepgpqg nqgpkgppgp aglkgdrgea gppgpigrdg lsgqrglpgs pgpvgvpged
     1081 gdkgdigppg ekgfkgsqge mgppgppglq glrgepgaig lpgekgppge igrqgpkged
     1141 gpvgasgppg pigpqglpgp aglkgevgdt gavgpvgpag tpgeqgprgp kgseglqgpp
     1201 gpegrqgekg dagapglpgp sgpegkpger gpqglkgeeg ksgppgplgs rgisgpegpk
     1261 gnagppgfpg ppgepglagl kgepgkdged gkagdpgppg eigapgkegl pglpgkqgpe
     1321 gpaglqgspg mpgekgdmgl pgapgiqgri gdqglpgppg lpglrglpgl agengplgma
     1381 gavgapgkag pigpkgpkgd rgkrgfkghr gelghvglkg eqgekgeqgp rgatgpegpk
     1441 gvqgpvglmg fkgndgppgl pgmegppgpk gnagndgpkg esgppgppgp pgppaeqpmi
     1501 ppellftreq ilrrkrdvpk etnepkeerk ppenvdkenq neeelgpkfl dmyssiyamr
     1561 qeldrirkpi gsrenpvrtc kdlfyghphf nngwywidpn lgmaddavyv ycnmtnmget
     1621 cvhpdihtsq mpnipwkken nktdwysnlr ggfkityeti gvvqlnflrl lsqeayqnft
     1681 ytcinsigwy ntlnddydss lrllgandde fsyagikpqv tmdncktrks kgetvflies
     1741 kimqqlplvd fypvdyglph qafgftvgpi cfk
//
LOCUS       XP_034182886             457 aa            linear   INV 03-SEP-2025
DEFINITION  WW domain-containing oxidoreductase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182886
VERSION     XP_034182886.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326995.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..457
                     /product="WW domain-containing oxidoreductase isoform X1"
                     /calculated_mol_wt=51985
     Region          57..86
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          99..129
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(113,124)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          163..441
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(170,172..173,175,194..196,249..251,303..305,331,335,
                     359..362)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(271,305,331,335)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..457
                     /gene="Wwox"
                     /coded_by="XM_034326995.2:16..1389"
                     /db_xref="GeneID:117605553"
ORIGIN      
        1 myetqhrkey kvqiicnkey lkttllaqsl nggkinasen yfllysilnd sdsedelppg
       61 weemttvdgn vyyvnhytkg tqwthprtgr kkivegelps gwercvsedg kvlfvdhmnr
      121 tttytdprla fateyressq pvrqrfdsss talsvlhgrd lrgkiaivtg antgigfeta
      181 rslalhgcrv ilacrdmkka eeavqkiqqe rdnvicetlh ldlsslhsvr eaaekfkqky
      241 rtlhililna gvfaipyqlt kdgyeatfqi nhlshfyftl llehpirsch nsrvvvvsse
      301 shrfsslrtv edfhqltlsp paykywfmga yndsklcnil faqelakcwp svsvfschpg
      361 nmvssslsry swtlrllfmf vrpftkslqq aastsvfcat apelegvtgv yfnncyrcdp
      421 snvaldpala arlwsvsqem iisimkkdkl wetlalk
//
LOCUS       XP_034182887             414 aa            linear   INV 03-SEP-2025
DEFINITION  WW domain-containing oxidoreductase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182887
VERSION     XP_034182887.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326996.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="WW domain-containing oxidoreductase isoform X2"
                     /calculated_mol_wt=46859
     Region          14..43
                     /region_name="WW"
                     /note="WW domain; pfam00397"
                     /db_xref="CDD:459800"
     Region          56..86
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(70,81)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          120..398
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(127,129..130,132,151..153,206..208,260..262,288,292,
                     316..319)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(228,262,288,292)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..414
                     /gene="Wwox"
                     /coded_by="XM_034326996.2:138..1382"
                     /db_xref="GeneID:117605553"
ORIGIN      
        1 migilndsds edelppgwee mttvdgnvyy vnhytkgtqw thprtgrkki vegelpsgwe
       61 rcvsedgkvl fvdhmnrttt ytdprlafat eyressqpvr qrfdssstal svlhgrdlrg
      121 kiaivtgant gigfetarsl alhgcrvila crdmkkaeea vqkiqqerdn vicetlhldl
      181 sslhsvreaa ekfkqkyrtl hililnagvf aipyqltkdg yeatfqinhl shfyftllle
      241 hpirschnsr vvvvsseshr fsslrtvedf hqltlsppay kywfmgaynd sklcnilfaq
      301 elakcwpsvs vfschpgnmv ssslsryswt lrllfmfvrp ftkslqqaas tsvfcatape
      361 legvtgvyfn ncyrcdpsnv aldpalaarl wsvsqemiis imkkdklwet lalk
//
LOCUS       XP_034182888             394 aa            linear   INV 03-SEP-2025
DEFINITION  WW domain-containing oxidoreductase isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182888
VERSION     XP_034182888.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326997.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..394
                     /product="WW domain-containing oxidoreductase isoform X3"
                     /calculated_mol_wt=44630
     Region          <2..24
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(9,20)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          36..66
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(50,61)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     Region          100..378
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(107,109..110,112,131..133,186..188,240..242,268,272,
                     296..299)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187535"
     Site            order(208,242,268,272)
                     /site_type="active"
                     /db_xref="CDD:187535"
     CDS             1..394
                     /gene="Wwox"
                     /coded_by="XM_034326997.2:127..1311"
                     /db_xref="GeneID:117605553"
ORIGIN      
        1 mttvdgnvyy vnhytkgtqw thprtgrkki vegelpsgwe rcvsedgkvl fvdhmnrttt
       61 ytdprlafat eyressqpvr qrfdssstal svlhgrdlrg kiaivtgant gigfetarsl
      121 alhgcrvila crdmkkaeea vqkiqqerdn vicetlhldl sslhsvreaa ekfkqkyrtl
      181 hililnagvf aipyqltkdg yeatfqinhl shfyftllle hpirschnsr vvvvsseshr
      241 fsslrtvedf hqltlsppay kywfmgaynd sklcnilfaq elakcwpsvs vfschpgnmv
      301 ssslsryswt lrllfmfvrp ftkslqqaas tsvfcatape legvtgvyfn ncyrcdpsnv
      361 aldpalaarl wsvsqemiis imkkdklwet lalk
//
LOCUS       XP_034182889             286 aa            linear   INV 03-SEP-2025
DEFINITION  MICOS complex subunit 26/27 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034182889
VERSION     XP_034182889.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326998.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="MICOS complex subunit 26/27 isoform X1"
                     /calculated_mol_wt=32252
     Region          100..217
                     /region_name="ApoO"
                     /note="Apolipoprotein O; pfam09769"
                     /db_xref="CDD:462891"
     CDS             1..286
                     /gene="Mic26-27"
                     /coded_by="XM_034326998.2:16..876"
                     /db_xref="GeneID:117605554"
ORIGIN      
        1 mhnyvhkfle stignvfgkd itcrepvhrc ddyqpgvlve fgrtgiklfk kflmpcglca
       61 avpamkpvns seertapcnt etqakklvkp selpiysvdd gynkqmpcig ypsiveenir
      121 kvretiqevk vtfnnisynv astvdqfrfi idylqdeanl mprigavgig glsglvlglr
      181 ggmfkrllyt ttgagiigci cfpkeakqtl nrvehygnis ynfvygvkpg nspqdislid
      241 mfhlknvfes eyfrmitfdk ffeqkkentt ekpeestpdk pekekk
//
LOCUS       XP_076548093             282 aa            linear   INV 03-SEP-2025
DEFINITION  MICOS complex subunit 26/27 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548093
VERSION     XP_076548093.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691978.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="MICOS complex subunit 26/27 isoform X2"
                     /calculated_mol_wt=31845
     Region          100..217
                     /region_name="ApoO"
                     /note="Apolipoprotein O; pfam09769"
                     /db_xref="CDD:462891"
     CDS             1..282
                     /gene="Mic26-27"
                     /coded_by="XM_076691978.1:16..864"
                     /db_xref="GeneID:117605554"
ORIGIN      
        1 mhnyvhkfle stignvfgkd itcrepvhrc ddyqpgvlve fgrtgiklfk kflmpcglca
       61 avpamkpvns seertapcnt etqakklvkp selpiysvdd gynkqmpcig ypsiveenir
      121 kvretiqevk vtfnnisynv astvdqfrfi idylqdeanl mprigavgig glsglvlglr
      181 ggmfkrllyt ttgagiigci cfpkeakqtl nrvehygnis ynfvygvkpg nspqdislid
      241 mfhlknvfes eyfrmitfdk ffeqkkentt ekpefwksll ks
//
LOCUS       XP_034182890             276 aa            linear   INV 03-SEP-2025
DEFINITION  MICOS complex subunit 26/27 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034182890
VERSION     XP_034182890.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326999.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="MICOS complex subunit 26/27 isoform X3"
                     /calculated_mol_wt=30963
     Region          100..217
                     /region_name="ApoO"
                     /note="Apolipoprotein O; pfam09769"
                     /db_xref="CDD:462891"
     CDS             1..276
                     /gene="Mic26-27"
                     /coded_by="XM_034326999.2:16..846"
                     /db_xref="GeneID:117605554"
ORIGIN      
        1 mhnyvhkfle stignvfgkd itcrepvhrc ddyqpgvlve fgrtgiklfk kflmpcglca
       61 avpamkpvns seertapcnt etqakklvkp selpiysvdd gynkqmpcig ypsiveenir
      121 kvretiqevk vtfnnisynv astvdqfrfi idylqdeanl mprigavgig glsglvlglr
      181 ggmfkrllyt ttgagiigci cfpkeakqtl nrvehygnis ynfvygvlek ppeelaetrl
      241 lsfhrnnenr skslesvtln eipnknpkfk efkkpd
//
LOCUS       XP_034182891             276 aa            linear   INV 03-SEP-2025
DEFINITION  MICOS complex subunit 26/27 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034182891
VERSION     XP_034182891.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327000.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..276
                     /product="MICOS complex subunit 26/27 isoform X3"
                     /calculated_mol_wt=30963
     Region          100..217
                     /region_name="ApoO"
                     /note="Apolipoprotein O; pfam09769"
                     /db_xref="CDD:462891"
     CDS             1..276
                     /gene="Mic26-27"
                     /coded_by="XM_034327000.2:16..846"
                     /db_xref="GeneID:117605554"
ORIGIN      
        1 mhnyvhkfle stignvfgkd itcrepvhrc ddyqpgvlve fgrtgiklfk kflmpcglca
       61 avpamkpvns seertapcnt etqakklvkp selpiysvdd gynkqmpcig ypsiveenir
      121 kvretiqevk vtfnnisynv astvdqfrfi idylqdeanl mprigavgig glsglvlglr
      181 ggmfkrllyt ttgagiigci cfpkeakqtl nrvehygnis ynfvygvlek ppeelaetrl
      241 lsfhrnnenr skslesvtln eipnknpkfk efkkpd
//
LOCUS       XP_034182892             244 aa            linear   INV 03-SEP-2025
DEFINITION  MICOS complex subunit 26/27 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034182892
VERSION     XP_034182892.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327001.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..244
                     /product="MICOS complex subunit 26/27 isoform X4"
                     /calculated_mol_wt=27510
     Region          58..175
                     /region_name="ApoO"
                     /note="Apolipoprotein O; pfam09769"
                     /db_xref="CDD:462891"
     CDS             1..244
                     /gene="Mic26-27"
                     /coded_by="XM_034327001.2:158..892"
                     /db_xref="GeneID:117605554"
ORIGIN      
        1 mqrtmlfkkf lmpcglcaav pamkpvnsse ertapcntet qakklvkpse lpiysvddgy
       61 nkqmpcigyp siveenirkv retiqevkvt fnnisynvas tvdqfrfiid ylqdeanlmp
      121 rigavgiggl sglvlglrgg mfkrllyttt gagiigcicf pkeakqtlnr vehygnisyn
      181 fvygvkpgns pqdislidmf hlknvfesey frmitfdkff eqkkenttek peestpdkpe
      241 kekk
//
LOCUS       XP_034182894             242 aa            linear   INV 03-SEP-2025
DEFINITION  MICOS complex subunit 26/27 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034182894
VERSION     XP_034182894.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..242
                     /product="MICOS complex subunit 26/27 isoform X5"
                     /calculated_mol_wt=27220
     Region          56..173
                     /region_name="ApoO"
                     /note="Apolipoprotein O; pfam09769"
                     /db_xref="CDD:462891"
     CDS             1..242
                     /gene="Mic26-27"
                     /coded_by="XM_034327003.2:140..868"
                     /db_xref="GeneID:117605554"
ORIGIN      
        1 millfkkflm pcglcaavpa mkpvnsseer tapcntetqa kklvkpselp iysvddgynk
       61 qmpcigypsi veenirkvre tiqevkvtfn nisynvastv dqfrfiidyl qdeanlmpri
      121 gavgigglsg lvlglrggmf krllytttga giigcicfpk eakqtlnrve hygnisynfv
      181 ygvkpgnspq dislidmfhl knvfeseyfr mitfdkffeq kkenttekpe estpdkpeke
      241 kk
//
LOCUS       XP_034182895             261 aa            linear   INV 03-SEP-2025
DEFINITION  DCN1-like protein SCCRO4 [Osmia lignaria lignaria].
ACCESSION   XP_034182895
VERSION     XP_034182895.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327004.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..261
                     /product="DCN1-like protein SCCRO4"
                     /calculated_mol_wt=30397
     Region          134..244
                     /region_name="Cullin_binding"
                     /note="Cullin binding; pfam03556"
                     /db_xref="CDD:460971"
     CDS             1..261
                     /gene="SCCRO4"
                     /coded_by="XM_034327004.2:489..1274"
                     /db_xref="GeneID:117605555"
ORIGIN      
        1 mprskrraps stnhhtsiem spsaheegip rhpskrlrht ssarrytkte dvssvssfsq
       61 krcitwfrey ttpddsdtlg pegmekfced igvepenvvm lvlaykmnar qmgfftlsew
      121 lkglselqcd siskiqqkle ylrnqlndph tfkgiyryay dfardkdqrs mdmetarvml
      181 qlllgkhwpl ftqfaqfldq skykvinkdq wcnilefsrt inhdlsnydl dgawpvmlde
      241 fvewlkmqrg eeaastetrg s
//
LOCUS       XP_034182896             261 aa            linear   INV 03-SEP-2025
DEFINITION  DCN1-like protein SCCRO4 [Osmia lignaria lignaria].
ACCESSION   XP_034182896
VERSION     XP_034182896.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327005.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..261
                     /product="DCN1-like protein SCCRO4"
                     /calculated_mol_wt=30397
     Region          134..244
                     /region_name="Cullin_binding"
                     /note="Cullin binding; pfam03556"
                     /db_xref="CDD:460971"
     CDS             1..261
                     /gene="SCCRO4"
                     /coded_by="XM_034327005.2:466..1251"
                     /db_xref="GeneID:117605555"
ORIGIN      
        1 mprskrraps stnhhtsiem spsaheegip rhpskrlrht ssarrytkte dvssvssfsq
       61 krcitwfrey ttpddsdtlg pegmekfced igvepenvvm lvlaykmnar qmgfftlsew
      121 lkglselqcd siskiqqkle ylrnqlndph tfkgiyryay dfardkdqrs mdmetarvml
      181 qlllgkhwpl ftqfaqfldq skykvinkdq wcnilefsrt inhdlsnydl dgawpvmlde
      241 fvewlkmqrg eeaastetrg s
//
LOCUS       XP_076548094             261 aa            linear   INV 03-SEP-2025
DEFINITION  DCN1-like protein SCCRO4 [Osmia lignaria lignaria].
ACCESSION   XP_076548094
VERSION     XP_076548094.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691979.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..261
                     /product="DCN1-like protein SCCRO4"
                     /calculated_mol_wt=30397
     Region          134..244
                     /region_name="Cullin_binding"
                     /note="Cullin binding; pfam03556"
                     /db_xref="CDD:460971"
     CDS             1..261
                     /gene="SCCRO4"
                     /coded_by="XM_076691979.1:20..805"
                     /db_xref="GeneID:117605555"
ORIGIN      
        1 mprskrraps stnhhtsiem spsaheegip rhpskrlrht ssarrytkte dvssvssfsq
       61 krcitwfrey ttpddsdtlg pegmekfced igvepenvvm lvlaykmnar qmgfftlsew
      121 lkglselqcd siskiqqkle ylrnqlndph tfkgiyryay dfardkdqrs mdmetarvml
      181 qlllgkhwpl ftqfaqfldq skykvinkdq wcnilefsrt inhdlsnydl dgawpvmlde
      241 fvewlkmqrg eeaastetrg s
//
LOCUS       XP_034182971            1991 aa            linear   INV 03-SEP-2025
DEFINITION  msps cytoskeleton-associated protein 5 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182971
VERSION     XP_034182971.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327080.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1991
                     /product="msps cytoskeleton-associated protein 5 isoform
                     X1"
                     /calculated_mol_wt=222201
     Site            order(297..298,301,304..305,309,338..339,342,345..346,349,
                     375..376,379,382..383,414..415,418,421..422)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          319..350
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          358..383
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          394..422
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          935..959
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          971..1003
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1013..1043
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1991
                     /gene="msps"
                     /coded_by="XM_034327080.2:255..6230"
                     /db_xref="GeneID:117605579"
ORIGIN      
        1 meqdteyikl pleercvhkl wrarlhgyke cvntfqcidd ekspewnkfl gfikkfvlds
       61 navaqekgle aalaflenaa vagktvgevm ngivtkciaa pktktkdlav qitlmyieie
      121 kheavqeell kgteaknpki vaacistltl alrqfgpkvi nikplikkip gfledrdkiv
      181 rdegkvmvve iyrwigtplk qqlntlkpvq lteletefnn lkqekvvpir flksqkpkvt
      241 nivdstsdng eecqddgdgg sipeldpyel lepvdilskl pkdfhekiea kkwqerkeal
      301 ealevlvknp klangdygdv vralkkvisk dtnvlvvtla gkclaglatg lkkrfqsyat
      361 aclssilekf rekkqtvvqa lreaadaifl svsidviled svaalenknp avkaetaayl
      421 arcfsrtppp slnkkllkay ttillktlne pdptvrdcsa dalgtamkli geksmmpflt
      481 dldnlkmtki kecaekavih vkipstskaa aerpntapsk iesaakskes efkvpkrpnt
      541 snakktsakk pssssltnla gskkasapkl qveknysaee idemamqllp gdilsglvds
      601 nwktrltave qlmefvkqtd ptevptqviv rtlakkpgfk dtnfqvlklr leivkylaen
      661 fpfsttvcey cimditeklg daknsavage tllaiaeats feyvadeiva fafnqknpkv
      721 qqetlsvlsr gltefgcvvn vkslmenikk avavtnpgvr tsaitllgtl ylfmgrpllt
      781 ffenekpalr qqieqecekh ngetppvpfr gvknkkdkan dddddvevek ksssnseidi
      841 nnlipridis nqitesllne ladknwkvrn eglqkintii sdakfikgsi gdlpqslalr
      901 lvdsnskiaq stlgicqala iamgppakqh irvlfpgfiq clgdsknwir taaiscintw
      961 gdqcgykeff dgemigdvlk sgspilraev wnwlaqklpl ipvkqvpkee llvclphlys
     1021 nledrnsdvr kyaqeavlgf mihlsyelma rnteklkpgs rtvvltaldk srpnlpikpl
     1081 pkkpppeesn qkvvksagam kaskavvkpk qnqaaskpgs arkkdddvdt spllainnlk
     1141 hqrvideqkl kvlkwnfttp reefvellke lmtaanvnkt llanmfhsdf ryhlkaieal
     1201 tedlpdnska lvsnldlilk wltlrffdtn psvllkgley lrmvfnllie dqyhmlenea
     1261 asfipyliik igdpkdavrn gvralfkqia lvypvsklfs yvmeglkskn arqrtecldq
     1321 lgslienygl svcqpsasva lkeiakqiad rdnsvrnaal ncivqayflq gervykligq
     1381 isekdqslld erikraaknr ptksasasrl sapsnatpvp snedikaese eeneeilepq
     1441 ptppsqpqin elphndppvn vttekpsdee qvspdsnkqp seivaeinvs padheddtkt
     1501 nsptstqpkv sgpfgldmdl lqrielnapv kyrnpvllev nlndlnespn alnpskvpvi
     1561 pisppkllvs ksatmsqqhi tttnaskeds lerkilamas ldlavaiqsm nsidnliksh
     1621 qilslqsked kfigsinmql kllqtyplqq ggadiskgfr ntfmvllafy dtgilgknvp
     1681 lihlkelvdq misllaenkl dhlhqaeayy rvinsivcki idnsnhttii cvlikllhgc
     1741 aestapskye elvmkclwki vktipnwaad ldydtillev hrflkdypsv wwkkrksdtp
     1801 lrtiktilhs mtrvkgstil shltrinntn eselhsylir liatfkpdei nsnpktsikl
     1861 sntgksqehl skfthqqlsa ifkkigskeh tqeglmqlyd fklqypeadv qpfllkshqf
     1921 fqdfieqglr didqarknqn lisqtnnqys tetaesaase ekslmdplnr veklrvseaq
     1981 crtttsqpsp t
//
LOCUS       XP_034182972            1991 aa            linear   INV 03-SEP-2025
DEFINITION  msps cytoskeleton-associated protein 5 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182972
VERSION     XP_034182972.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327081.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1991
                     /product="msps cytoskeleton-associated protein 5 isoform
                     X1"
                     /calculated_mol_wt=222201
     Site            order(297..298,301,304..305,309,338..339,342,345..346,349,
                     375..376,379,382..383,414..415,418,421..422)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          319..350
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          358..383
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          394..422
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          935..959
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          971..1003
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1013..1043
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1991
                     /gene="msps"
                     /coded_by="XM_034327081.2:29..6004"
                     /db_xref="GeneID:117605579"
ORIGIN      
        1 meqdteyikl pleercvhkl wrarlhgyke cvntfqcidd ekspewnkfl gfikkfvlds
       61 navaqekgle aalaflenaa vagktvgevm ngivtkciaa pktktkdlav qitlmyieie
      121 kheavqeell kgteaknpki vaacistltl alrqfgpkvi nikplikkip gfledrdkiv
      181 rdegkvmvve iyrwigtplk qqlntlkpvq lteletefnn lkqekvvpir flksqkpkvt
      241 nivdstsdng eecqddgdgg sipeldpyel lepvdilskl pkdfhekiea kkwqerkeal
      301 ealevlvknp klangdygdv vralkkvisk dtnvlvvtla gkclaglatg lkkrfqsyat
      361 aclssilekf rekkqtvvqa lreaadaifl svsidviled svaalenknp avkaetaayl
      421 arcfsrtppp slnkkllkay ttillktlne pdptvrdcsa dalgtamkli geksmmpflt
      481 dldnlkmtki kecaekavih vkipstskaa aerpntapsk iesaakskes efkvpkrpnt
      541 snakktsakk pssssltnla gskkasapkl qveknysaee idemamqllp gdilsglvds
      601 nwktrltave qlmefvkqtd ptevptqviv rtlakkpgfk dtnfqvlklr leivkylaen
      661 fpfsttvcey cimditeklg daknsavage tllaiaeats feyvadeiva fafnqknpkv
      721 qqetlsvlsr gltefgcvvn vkslmenikk avavtnpgvr tsaitllgtl ylfmgrpllt
      781 ffenekpalr qqieqecekh ngetppvpfr gvknkkdkan dddddvevek ksssnseidi
      841 nnlipridis nqitesllne ladknwkvrn eglqkintii sdakfikgsi gdlpqslalr
      901 lvdsnskiaq stlgicqala iamgppakqh irvlfpgfiq clgdsknwir taaiscintw
      961 gdqcgykeff dgemigdvlk sgspilraev wnwlaqklpl ipvkqvpkee llvclphlys
     1021 nledrnsdvr kyaqeavlgf mihlsyelma rnteklkpgs rtvvltaldk srpnlpikpl
     1081 pkkpppeesn qkvvksagam kaskavvkpk qnqaaskpgs arkkdddvdt spllainnlk
     1141 hqrvideqkl kvlkwnfttp reefvellke lmtaanvnkt llanmfhsdf ryhlkaieal
     1201 tedlpdnska lvsnldlilk wltlrffdtn psvllkgley lrmvfnllie dqyhmlenea
     1261 asfipyliik igdpkdavrn gvralfkqia lvypvsklfs yvmeglkskn arqrtecldq
     1321 lgslienygl svcqpsasva lkeiakqiad rdnsvrnaal ncivqayflq gervykligq
     1381 isekdqslld erikraaknr ptksasasrl sapsnatpvp snedikaese eeneeilepq
     1441 ptppsqpqin elphndppvn vttekpsdee qvspdsnkqp seivaeinvs padheddtkt
     1501 nsptstqpkv sgpfgldmdl lqrielnapv kyrnpvllev nlndlnespn alnpskvpvi
     1561 pisppkllvs ksatmsqqhi tttnaskeds lerkilamas ldlavaiqsm nsidnliksh
     1621 qilslqsked kfigsinmql kllqtyplqq ggadiskgfr ntfmvllafy dtgilgknvp
     1681 lihlkelvdq misllaenkl dhlhqaeayy rvinsivcki idnsnhttii cvlikllhgc
     1741 aestapskye elvmkclwki vktipnwaad ldydtillev hrflkdypsv wwkkrksdtp
     1801 lrtiktilhs mtrvkgstil shltrinntn eselhsylir liatfkpdei nsnpktsikl
     1861 sntgksqehl skfthqqlsa ifkkigskeh tqeglmqlyd fklqypeadv qpfllkshqf
     1921 fqdfieqglr didqarknqn lisqtnnqys tetaesaase ekslmdplnr veklrvseaq
     1981 crtttsqpsp t
//
LOCUS       XP_034182973            1943 aa            linear   INV 03-SEP-2025
DEFINITION  msps cytoskeleton-associated protein 5 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182973
VERSION     XP_034182973.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327082.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1943
                     /product="msps cytoskeleton-associated protein 5 isoform
                     X2"
                     /calculated_mol_wt=216981
     Region          935..959
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          971..1003
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          1013..1043
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..1943
                     /gene="msps"
                     /coded_by="XM_034327082.2:254..6085"
                     /db_xref="GeneID:117605579"
ORIGIN      
        1 meqdteyikl pleercvhkl wrarlhgyke cvntfqcidd ekspewnkfl gfikkfvlds
       61 navaqekgle aalaflenaa vagktvgevm ngivtkciaa pktktkdlav qitlmyieie
      121 kheavqeell kgteaknpki vaacistltl alrqfgpkvi nikplikkip gfledrdkiv
      181 rdegkvmvve iyrwigtplk qqlntlkpvq lteletefnn lkqekvvpir flksqkpkvt
      241 nivdstsdng eecqddgdgg sipeldpyel lepvdilskl pkdfhekiea kkwqerkeal
      301 ealevlvknp klangdygdv vralkkvisk dtnvlvvtla gkclaglatg lkkrfqsyat
      361 aclssilekf rekkqtvvqa lreaadaifl svsidviled svaalenknp avkaetaayl
      421 arcfsrtppp slnkkllkay ttillktlne pdptvrdcsa dalgtamkli geksmmpflt
      481 dldnlkmtki kecaekavih vkipstskaa aerpntapsk iesaakskes efkvpkrpnt
      541 snakktsakk pssssltnla gskkasapkl qveknysaee idemamqllp gdilsglvds
      601 nwktrltave qlmefvkqtd ptevptqviv rtlakkpgfk dtnfqvlklr leivkylaen
      661 fpfsttvcey cimditeklg daknsavage tllaiaeats feyvadeiva fafnqknpkv
      721 qqetlsvlsr gltefgcvvn vkslmenikk avavtnpgvr tsaitllgtl ylfmgrpllt
      781 ffenekpalr qqieqecekh ngetppvpfr gvknkkdkan dddddvevek ksssnseidi
      841 nnlipridis nqitesllne ladknwkvrn eglqkintii sdakfikgsi gdlpqslalr
      901 lvdsnskiaq stlgicqala iamgppakqh irvlfpgfiq clgdsknwir taaiscintw
      961 gdqcgykeff dgemigdvlk sgspilraev wnwlaqklpl ipvkqvpkee llvclphlys
     1021 nledrnsdvr kyaqeavlgf mihlsyelma rnteklkpgs rtvvltaldk srpnlpikpl
     1081 pkkpppeesn qkvvksagam kaskavvkpk qnqaaskpgs arkkdddvdt spllainnlk
     1141 hqrvideqkl kvlkwnfttp reefvellke lmtaanvnkt llanmfhsdf ryhlkaieal
     1201 tedlpdnska lvsnldlilk wltlrffdtn psvllkgley lrmvfnllie dqyhmlenea
     1261 asfipyliik igdpkdavrn gvralfkqia lvypvsklfs yvmeglkskn arqrtecldq
     1321 lgslienygl svcqpsasva lkeiakqiad rdnsvrnaal ncivqayflq gervykligq
     1381 isekdqslld erikraaknr ptksasasrl sapsnatpvp snedikaese eeneeilepq
     1441 ptppsqpqin elnsptstqp kvsgpfgldm dllqrielna pvkyrnpvll evnlndlnes
     1501 pnalnpskvp vipisppkll vsksatmsqq hitttnaske dslerkilam asldlavaiq
     1561 smnsidnlik shqilslqsk edkfigsinm qlkllqtypl qqggadiskg frntfmvlla
     1621 fydtgilgkn vplihlkelv dqmisllaen kldhlhqaea yyrvinsivc kiidnsnhtt
     1681 iicvlikllh gcaestapsk yeelvmkclw kivktipnwa adldydtill evhrflkdyp
     1741 svwwkkrksd tplrtiktil hsmtrvkgst ilshltrinn tneselhsyl irliatfkpd
     1801 einsnpktsi klsntgksqe hlskfthqql saifkkigsk ehtqeglmql ydfklqypea
     1861 dvqpfllksh qffqdfieqg lrdidqarkn qnlisqtnnq ystetaesaa seekslmdpl
     1921 nrveklrvse aqcrtttsqp spt
//
LOCUS       XP_034182974             292 aa            linear   INV 03-SEP-2025
DEFINITION  putative divalent cation/proton antiporter TMEM165 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182974
VERSION     XP_034182974.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327083.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="putative divalent cation/proton antiporter
                     TMEM165"
                     /calculated_mol_wt=32255
     Region          61..285
                     /region_name="Gdt1"
                     /note="Putative Ca2+/H+ antiporter, TMEM165/GDT1 family
                     [General function prediction only]; COG2119"
                     /db_xref="CDD:441722"
     CDS             1..292
                     /gene="LOC117605580"
                     /coded_by="XM_034327083.2:217..1095"
                     /db_xref="GeneID:117605580"
ORIGIN      
        1 mglnlftlns enifclcaig yfllpivsie sesryndkfe imttmkpkde ilsqtkdnlg
       61 flhaliasls vivvselgdk tffiaaimam khprltifvg aisalalmti lsvifgyaat
      121 iiprtytyyi stalfalfgl kmlrdgykms ateaqeelee vqsdlrkrdd eyeketattl
      181 vqdpetgvir katkisalml lsriffqaft ltflaewgdr sqlttiilaa redvygvvig
      241 gilghmfctg lavlggrmia qkisirtvti igglvfllfa ftalfispte di
//
LOCUS       XP_034182975             292 aa            linear   INV 03-SEP-2025
DEFINITION  putative divalent cation/proton antiporter TMEM165 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182975
VERSION     XP_034182975.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327084.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..292
                     /product="putative divalent cation/proton antiporter
                     TMEM165"
                     /calculated_mol_wt=32255
     Region          61..285
                     /region_name="Gdt1"
                     /note="Putative Ca2+/H+ antiporter, TMEM165/GDT1 family
                     [General function prediction only]; COG2119"
                     /db_xref="CDD:441722"
     CDS             1..292
                     /gene="LOC117605580"
                     /coded_by="XM_034327084.2:57..935"
                     /db_xref="GeneID:117605580"
ORIGIN      
        1 mglnlftlns enifclcaig yfllpivsie sesryndkfe imttmkpkde ilsqtkdnlg
       61 flhaliasls vivvselgdk tffiaaimam khprltifvg aisalalmti lsvifgyaat
      121 iiprtytyyi stalfalfgl kmlrdgykms ateaqeelee vqsdlrkrdd eyeketattl
      181 vqdpetgvir katkisalml lsriffqaft ltflaewgdr sqlttiilaa redvygvvig
      241 gilghmfctg lavlggrmia qkisirtvti igglvfllfa ftalfispte di
//
LOCUS       XP_034182976             486 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein Elbow isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034182976
VERSION     XP_034182976.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327085.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="zinc finger protein Elbow isoform X1"
                     /calculated_mol_wt=50198
     Region          353..382
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          355..382
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..486
                     /gene="LOC117605581"
                     /coded_by="XM_034327085.2:373..1833"
                     /db_xref="GeneID:117605581"
ORIGIN      
        1 mltssanqyl rpeyltplpt tldakkspla llaqtcsqig adspsnksll gsldkassnk
       61 ssksdqsrek ssptvapssl estktnfkpy esclarekas tpdeqrsass hsasgrsrtp
      121 gsgnkrcpsn qsvssvravt pqgrktatpn gdvarespas rtsatgnlss nqleptcsss
      181 qpavvsspsl qakpsyspas vlaitdpsik dlplgtfkpg vslasstaay ltyapqlpid
      241 amastlvsqh haslknglia gnpylsyarl ksssapdtlm pvcrdpyctg cqlnshlfan
      301 gklastttst ptscpagcaq cdhkpavayg algaaayaha qlaalaaasq lpyvcnwiag
      361 dtaycgkrfs tseellqhlr shttsstsaa tdpsaaalsl lspsvglppt hplfsrtypt
      421 pplsplatar yhpygksstl lppslsslgl plppphshtp aglppyfspy slygaprlga
      481 asglpq
//
LOCUS       XP_034182977             481 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein 703 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182977
VERSION     XP_034182977.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327086.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="zinc finger protein 703 isoform X2"
                     /calculated_mol_wt=49611
     Region          348..377
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          350..377
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..481
                     /gene="LOC117605581"
                     /coded_by="XM_034327086.2:15..1460"
                     /db_xref="GeneID:117605581"
ORIGIN      
        1 mstrsslvvi rsrsckldak ksplallaqt csqigadsps nksllgsldk assnkssksd
       61 qsrekssptv apsslestkt nfkpyescla rekastpdeq rsasshsasg rsrtpgsgnk
      121 rcpsnqsvss vravtpqgrk tatpngdvar espasrtsat gnlssnqlep tcsssqpavv
      181 sspslqakps yspasvlait dpsikdlplg tfkpgvslas staayltyap qlpidamast
      241 lvsqhhaslk ngliagnpyl syarlksssa pdtlmpvcrd pyctgcqlns hlfangklas
      301 tttstptscp agcaqcdhkp avaygalgaa ayahaqlaal aaasqlpyvc nwiagdtayc
      361 gkrfstseel lqhlrshtts stsaatdpsa aalsllspsv glppthplfs rtyptpplsp
      421 lataryhpyg ksstllppsl sslglplppp hshtpaglpp yfspyslyga prlgaasglp
      481 q
//
LOCUS       XP_034182978             469 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein 703 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034182978
VERSION     XP_034182978.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327087.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="zinc finger protein 703 isoform X3"
                     /calculated_mol_wt=48321
     Region          336..365
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          338..365
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..469
                     /gene="LOC117605581"
                     /coded_by="XM_034327087.2:125..1534"
                     /db_xref="GeneID:117605581"
ORIGIN      
        1 mqenldakks plallaqtcs qigadspsnk sllgsldkas snkssksdqs rekssptvap
       61 sslestktnf kpyesclare kastpdeqrs asshsasgrs rtpgsgnkrc psnqsvssvr
      121 avtpqgrkta tpngdvares pasrtsatgn lssnqleptc sssqpavvss pslqakpsys
      181 pasvlaitdp sikdlplgtf kpgvslasst aayltyapql pidamastlv sqhhaslkng
      241 liagnpylsy arlksssapd tlmpvcrdpy ctgcqlnshl fangklastt tstptscpag
      301 caqcdhkpav aygalgaaay ahaqlaalaa asqlpyvcnw iagdtaycgk rfstseellq
      361 hlrshttsst saatdpsaaa lsllspsvgl ppthplfsrt yptpplspla taryhpygks
      421 stllppslss lglplppphs htpaglppyf spyslygapr lgaasglpq
//
LOCUS       XP_034191175             350 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin G isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191175
VERSION     XP_034191175.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335284.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191175.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="cyclin G isoform X1"
                     /calculated_mol_wt=38823
     Region          49..139
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..350
                     /gene="CycG"
                     /coded_by="XM_034335284.2:18..1070"
                     /db_xref="GeneID:117609225"
ORIGIN      
        1 mgtsglpvpd aihplleqlq ealvlevkyq pnlqlptmsq teeitigard gsahvlrclk
       61 vwydlpsdvf fvainlmdrf ltkmkarpkh macisvsafh iaavqlaqsl dadhlvsisq
      121 ckctggdlkr mseviqnkle wapgtqpits ltflrlfnam frtvatelgs geaytsmite
      181 selllrlemv acdgncaslk psevalvllc iyldnavnrl dtntdhtisv tvtssssain
      241 asvtttrkhq mlrvlefave lqklckisdt sffstheavg avlskynaqe qtphrqrliw
      301 klssrtarll rptdkfisvl pviaehapvp spskirknrk fgrrhgnkrr
//
LOCUS       XP_034191176             350 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin G isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191176
VERSION     XP_034191176.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335285.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191176.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="cyclin G isoform X1"
                     /calculated_mol_wt=38823
     Region          49..139
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..350
                     /gene="CycG"
                     /coded_by="XM_034335285.2:18..1070"
                     /db_xref="GeneID:117609225"
ORIGIN      
        1 mgtsglpvpd aihplleqlq ealvlevkyq pnlqlptmsq teeitigard gsahvlrclk
       61 vwydlpsdvf fvainlmdrf ltkmkarpkh macisvsafh iaavqlaqsl dadhlvsisq
      121 ckctggdlkr mseviqnkle wapgtqpits ltflrlfnam frtvatelgs geaytsmite
      181 selllrlemv acdgncaslk psevalvllc iyldnavnrl dtntdhtisv tvtssssain
      241 asvtttrkhq mlrvlefave lqklckisdt sffstheavg avlskynaqe qtphrqrliw
      301 klssrtarll rptdkfisvl pviaehapvp spskirknrk fgrrhgnkrr
//
LOCUS       XP_076548095             284 aa            linear   INV 03-SEP-2025
DEFINITION  cyclin G isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548095
VERSION     XP_076548095.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691980.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..284
                     /product="cyclin G isoform X2"
                     /calculated_mol_wt=31428
     Region          49..139
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..284
                     /gene="CycG"
                     /coded_by="XM_076691980.1:294..1148"
                     /db_xref="GeneID:117609225"
ORIGIN      
        1 mgtsglpvpd aihplleqlq ealvlevkyq pnlqlptmsq teeitigard gsahvlrclk
       61 vwydlpsdvf fvainlmdrf ltkmkarpkh macisvsafh iaavqlaqsl dadhlvsisq
      121 ckctggdlkr mseviqnkle wapgtqpits ltflrlfnam frtvatelgs geaytsmite
      181 selllrlemv acdgncaslk psevalvllc iyldnavnrl dtntdhtisv tvtssssain
      241 asvtttrkhq mlrvlefave lqklyfrykf lfyarscgcc ikqi
//
LOCUS       XP_034194172             769 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation factor elongin A isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194172
VERSION     XP_034194172.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338281.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..769
                     /product="transcription elongation factor elongin A
                     isoform X1"
                     /calculated_mol_wt=86849
     Region          26..75
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          <96..396
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          589..673
                     /region_name="Elongin_A"
                     /note="RNA polymerase II transcription factor SIII
                     (Elongin) subunit A; pfam06881"
                     /db_xref="CDD:462026"
     CDS             1..769
                     /gene="EloA"
                     /coded_by="XM_034338281.2:203..2512"
                     /db_xref="GeneID:117610655"
ORIGIN      
        1 msvvdkikhy qrniekcddn edrilhcisk lsnlpvtvqh lqetgvgrtv nalrkyeggi
       61 geaakalvak wktmvanees segededeac vpdapesynd sresprsees siklksensg
      121 khvhrhkiek aevshtsyns kqsskheskv kpiefqsksn cdvktldndl ekqkkydkhk
      181 hshrhesrnd kkfskdnksa sknddthgsk hhthsksdnl ekysnrsscn snlpknneen
      241 hkkrknndsn asrkeskrrk nseseneegk fdvsfnsekh dantmldvik vkeepkekdi
      301 lnkseiklek kesqekikgs ssrhktdsss nklkesgekf khtsethtss nkdetkktdn
      361 hynnkesses khnnssshdk hcdknkgssk kkerkegisk hesksskdsk yskhgskepk
      421 elvkikqemn gdegidchsg asfaealgmc tmaqlskkrs nnspsssstk tvkteqlitn
      481 skktalktes atesvqtpsl lssamklepl nvdlastlpe isphykplpy vnpvhrkeed
      541 kaltdvmyvk nqrtkvysgn ksgytsvptl felctrvlie nidaleftgg vpfdilkpil
      601 eratpdqlfm lehynpylie dtdclwqfhc nrefrnkqre emetwremym rcldereakl
      661 kaltanikqs idksvpvrsa klayvdnivk pprnvlkkqa kygtancvps tasslkkkli
      721 sggnpadatn isvppppisr akpstgvvkk tkaplmakal qlikgrykr
//
LOCUS       XP_034194171             767 aa            linear   INV 03-SEP-2025
DEFINITION  transcription elongation factor elongin A isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034194171
VERSION     XP_034194171.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338280.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..767
                     /product="transcription elongation factor elongin A
                     isoform X2"
                     /calculated_mol_wt=86621
     Region          26..75
                     /region_name="Med26"
                     /note="TFIIS helical bundle-like domain; pfam08711"
                     /db_xref="CDD:462573"
     Region          <96..396
                     /region_name="MSCRAMM_ClfA"
                     /note="MSCRAMM family adhesin clumping factor ClfA;
                     NF033609"
                     /db_xref="CDD:468110"
     Region          587..671
                     /region_name="Elongin_A"
                     /note="RNA polymerase II transcription factor SIII
                     (Elongin) subunit A; pfam06881"
                     /db_xref="CDD:462026"
     CDS             1..767
                     /gene="EloA"
                     /coded_by="XM_034338280.2:204..2507"
                     /db_xref="GeneID:117610655"
ORIGIN      
        1 msvvdkikhy qrniekcddn edrilhcisk lsnlpvtvqh lqetgvgrtv nalrkyeggi
       61 geaakalvak wktmvanees segededeac vpdapesynd sresprsees siklksensg
      121 khvhrhkiek aevshtsyns kqsskheskv kpiefqsksn cdvktldndl ekqkkydkhk
      181 hshrhesrnd kkfskdnksa sknddthgsk hhthsksdnl ekysnrsscn snlpknneen
      241 hkkrknndsn asrkeskrrk nseseneegk fdvsfnsekh dantmldvik vkeepkekdi
      301 lnkseiklek kesqekikgs ssrhktdsss nklkesgekf khtsethtss nkdetkktdn
      361 hynnkesses khnnssshdk hcdknkgssk kkerkegisk hesksskdsk yskhgskepk
      421 elvkikqemn gdegidchsg asfaealgmc tmaqlskkrs nnspsssstk tvkteqlitn
      481 skktalktes atestpslls samkleplnv dlastlpeis phykplpyvn pvhrkeedka
      541 ltdvmyvknq rtkvysgnks gytsvptlfe lctrvlieni daleftggvp fdilkpiler
      601 atpdqlfmle hynpyliedt dclwqfhcnr efrnkqreem etwremymrc ldereaklka
      661 ltanikqsid ksvpvrsakl ayvdnivkpp rnvlkkqaky gtancvpsta sslkkklisg
      721 gnpadatnis vppppisrak pstgvvkktk aplmakalql ikgrykr
//
LOCUS       XP_076548096             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609127 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548096
VERSION     XP_076548096.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691981.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117609127 isoform X1"
                     /calculated_mol_wt=65072
     Region          46..104
                     /region_name="FLYWCH"
                     /note="FLYWCH zinc finger domain; pfam04500"
                     /db_xref="CDD:461332"
     Region          282..365
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <357..461
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..577
                     /gene="LOC117609127"
                     /coded_by="XM_076691981.1:184..1917"
                     /db_xref="GeneID:117609127"
ORIGIN      
        1 mrnnkmpqrv ksasnenkvk ieeeastsfp ltensgeddp nvaevvlsnk ggpklihrgf
       61 mytlhkkqpy nirwrcvgrt mhcrgslitt tsctkprvrm ehnhkpdfaa aqvarkryla
      121 lgkpcvltnl egnqnsmnap qrmihkendv peeqegrrrt trttarkvlq spsqsnttgk
      181 tiriaaqslv rphlrniapa piqqqqqqqq qqrqpktlvl ktiplkvkqv apttikmppr
      241 qttqglphni vhqqptevcl rwnsyhsnmq nsfpslldse qfvdvtlace grwlkchkmi
      301 lsscsdylad llrenpcqhp iilmkdlkfw evealvkfmy rgevnvahdk lpqllnaaea
      361 lqvkglagpn pssqnpkppl lipqskpvsq pvprassetk ekastsgvtt psspkraqkr
      421 qhteptepkp ftkirlqrpv tptspgvtvk mepldiplsp teifpetted cstpsnfdkl
      481 mslheeddpg gnevidgere infceipepp dindsedqme fvptdfldqe qdiveeteta
      541 snkdnkeesr dyssveledg ersenekepc skekkpv
//
LOCUS       XP_034190930             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609127 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190930
VERSION     XP_034190930.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335039.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190930.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117609127 isoform X1"
                     /calculated_mol_wt=65072
     Region          46..104
                     /region_name="FLYWCH"
                     /note="FLYWCH zinc finger domain; pfam04500"
                     /db_xref="CDD:461332"
     Region          282..365
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <357..461
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..577
                     /gene="LOC117609127"
                     /coded_by="XM_034335039.2:78..1811"
                     /db_xref="GeneID:117609127"
ORIGIN      
        1 mrnnkmpqrv ksasnenkvk ieeeastsfp ltensgeddp nvaevvlsnk ggpklihrgf
       61 mytlhkkqpy nirwrcvgrt mhcrgslitt tsctkprvrm ehnhkpdfaa aqvarkryla
      121 lgkpcvltnl egnqnsmnap qrmihkendv peeqegrrrt trttarkvlq spsqsnttgk
      181 tiriaaqslv rphlrniapa piqqqqqqqq qqrqpktlvl ktiplkvkqv apttikmppr
      241 qttqglphni vhqqptevcl rwnsyhsnmq nsfpslldse qfvdvtlace grwlkchkmi
      301 lsscsdylad llrenpcqhp iilmkdlkfw evealvkfmy rgevnvahdk lpqllnaaea
      361 lqvkglagpn pssqnpkppl lipqskpvsq pvprassetk ekastsgvtt psspkraqkr
      421 qhteptepkp ftkirlqrpv tptspgvtvk mepldiplsp teifpetted cstpsnfdkl
      481 mslheeddpg gnevidgere infceipepp dindsedqme fvptdfldqe qdiveeteta
      541 snkdnkeesr dyssveledg ersenekepc skekkpv
//
LOCUS       XP_034190931             577 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609127 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190931
VERSION     XP_034190931.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335040.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190931.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="uncharacterized protein LOC117609127 isoform X1"
                     /calculated_mol_wt=65072
     Region          46..104
                     /region_name="FLYWCH"
                     /note="FLYWCH zinc finger domain; pfam04500"
                     /db_xref="CDD:461332"
     Region          282..365
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <357..461
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..577
                     /gene="LOC117609127"
                     /coded_by="XM_034335040.2:181..1914"
                     /db_xref="GeneID:117609127"
ORIGIN      
        1 mrnnkmpqrv ksasnenkvk ieeeastsfp ltensgeddp nvaevvlsnk ggpklihrgf
       61 mytlhkkqpy nirwrcvgrt mhcrgslitt tsctkprvrm ehnhkpdfaa aqvarkryla
      121 lgkpcvltnl egnqnsmnap qrmihkendv peeqegrrrt trttarkvlq spsqsnttgk
      181 tiriaaqslv rphlrniapa piqqqqqqqq qqrqpktlvl ktiplkvkqv apttikmppr
      241 qttqglphni vhqqptevcl rwnsyhsnmq nsfpslldse qfvdvtlace grwlkchkmi
      301 lsscsdylad llrenpcqhp iilmkdlkfw evealvkfmy rgevnvahdk lpqllnaaea
      361 lqvkglagpn pssqnpkppl lipqskpvsq pvprassetk ekastsgvtt psspkraqkr
      421 qhteptepkp ftkirlqrpv tptspgvtvk mepldiplsp teifpetted cstpsnfdkl
      481 mslheeddpg gnevidgere infceipepp dindsedqme fvptdfldqe qdiveeteta
      541 snkdnkeesr dyssveledg ersenekepc skekkpv
//
LOCUS       XP_034190934             519 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609127 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190934
VERSION     XP_034190934.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335043.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190934.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="uncharacterized protein LOC117609127 isoform X2"
                     /calculated_mol_wt=58107
     Region          51..99
                     /region_name="zf-BED"
                     /note="BED zinc finger; pfam02892"
                     /db_xref="CDD:427043"
     Region          224..307
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <299..403
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..519
                     /gene="LOC117609127"
                     /coded_by="XM_034335043.2:886..2445"
                     /db_xref="GeneID:117609127"
ORIGIN      
        1 mkqepededd cylegaemss sspppltnrh tvsthrvrsh arslramtnp savwahfdlc
       61 andplraqcr icgavvvrgg anprqcgttn lhrhlrvhhg grligsryhv lqspsqsntt
      121 gktiriaaqs lvrphlrnia papiqqqqqq qqqqrqpktl vlktiplkvk qvapttikmp
      181 prqttqglph nivhqqptev clrwnsyhsn mqnsfpslld seqfvdvtla cegrwlkchk
      241 milsscsdyl adllrenpcq hpiilmkdlk fwevealvkf myrgevnvah dklpqllnaa
      301 ealqvkglag pnpssqnpkp pllipqskpv sqpvprasse tkekastsgv ttpsspkraq
      361 krqhteptep kpftkirlqr pvtptspgvt vkmepldipl spteifpett edcstpsnfd
      421 klmslheedd pggnevidge reinfceipe ppdindsedq mefvptdfld qeqdiveete
      481 tasnkdnkee srdyssvele dgerseneke pcskekkpv
//
LOCUS       XP_034190935             519 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609127 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190935
VERSION     XP_034190935.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335044.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190935.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="uncharacterized protein LOC117609127 isoform X2"
                     /calculated_mol_wt=58107
     Region          51..99
                     /region_name="zf-BED"
                     /note="BED zinc finger; pfam02892"
                     /db_xref="CDD:427043"
     Region          224..307
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <299..403
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..519
                     /gene="LOC117609127"
                     /coded_by="XM_034335044.2:1019..2578"
                     /db_xref="GeneID:117609127"
ORIGIN      
        1 mkqepededd cylegaemss sspppltnrh tvsthrvrsh arslramtnp savwahfdlc
       61 andplraqcr icgavvvrgg anprqcgttn lhrhlrvhhg grligsryhv lqspsqsntt
      121 gktiriaaqs lvrphlrnia papiqqqqqq qqqqrqpktl vlktiplkvk qvapttikmp
      181 prqttqglph nivhqqptev clrwnsyhsn mqnsfpslld seqfvdvtla cegrwlkchk
      241 milsscsdyl adllrenpcq hpiilmkdlk fwevealvkf myrgevnvah dklpqllnaa
      301 ealqvkglag pnpssqnpkp pllipqskpv sqpvprasse tkekastsgv ttpsspkraq
      361 krqhteptep kpftkirlqr pvtptspgvt vkmepldipl spteifpett edcstpsnfd
      421 klmslheedd pggnevidge reinfceipe ppdindsedq mefvptdfld qeqdiveete
      481 tasnkdnkee srdyssvele dgerseneke pcskekkpv
//
LOCUS       XP_076548097             519 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609127 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548097
VERSION     XP_076548097.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691982.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="uncharacterized protein LOC117609127 isoform X2"
                     /calculated_mol_wt=58107
     Region          51..99
                     /region_name="zf-BED"
                     /note="BED zinc finger; pfam02892"
                     /db_xref="CDD:427043"
     Region          224..307
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <299..403
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..519
                     /gene="LOC117609127"
                     /coded_by="XM_076691982.1:599..2158"
                     /db_xref="GeneID:117609127"
ORIGIN      
        1 mkqepededd cylegaemss sspppltnrh tvsthrvrsh arslramtnp savwahfdlc
       61 andplraqcr icgavvvrgg anprqcgttn lhrhlrvhhg grligsryhv lqspsqsntt
      121 gktiriaaqs lvrphlrnia papiqqqqqq qqqqrqpktl vlktiplkvk qvapttikmp
      181 prqttqglph nivhqqptev clrwnsyhsn mqnsfpslld seqfvdvtla cegrwlkchk
      241 milsscsdyl adllrenpcq hpiilmkdlk fwevealvkf myrgevnvah dklpqllnaa
      301 ealqvkglag pnpssqnpkp pllipqskpv sqpvprasse tkekastsgv ttpsspkraq
      361 krqhteptep kpftkirlqr pvtptspgvt vkmepldipl spteifpett edcstpsnfd
      421 klmslheedd pggnevidge reinfceipe ppdindsedq mefvptdfld qeqdiveete
      481 tasnkdnkee srdyssvele dgerseneke pcskekkpv
//
LOCUS       XP_034190936             519 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609127 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190936
VERSION     XP_034190936.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335045.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190936.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="uncharacterized protein LOC117609127 isoform X2"
                     /calculated_mol_wt=58107
     Region          51..99
                     /region_name="zf-BED"
                     /note="BED zinc finger; pfam02892"
                     /db_xref="CDD:427043"
     Region          224..307
                     /region_name="BTB_POZ_BAB-like"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac) /POZ
                     (poxvirus and zinc finger) domain found in Drosophila
                     melanogaster proteins bric-a-brac 1 (BAB1), bric-a-brac 2
                     (BAB2), modifier of mdg4 (doom), and similar proteins;
                     cd18315"
                     /db_xref="CDD:349624"
     Region          <299..403
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..519
                     /gene="LOC117609127"
                     /coded_by="XM_034335045.2:540..2099"
                     /db_xref="GeneID:117609127"
ORIGIN      
        1 mkqepededd cylegaemss sspppltnrh tvsthrvrsh arslramtnp savwahfdlc
       61 andplraqcr icgavvvrgg anprqcgttn lhrhlrvhhg grligsryhv lqspsqsntt
      121 gktiriaaqs lvrphlrnia papiqqqqqq qqqqrqpktl vlktiplkvk qvapttikmp
      181 prqttqglph nivhqqptev clrwnsyhsn mqnsfpslld seqfvdvtla cegrwlkchk
      241 milsscsdyl adllrenpcq hpiilmkdlk fwevealvkf myrgevnvah dklpqllnaa
      301 ealqvkglag pnpssqnpkp pllipqskpv sqpvprasse tkekastsgv ttpsspkraq
      361 krqhteptep kpftkirlqr pvtptspgvt vkmepldipl spteifpett edcstpsnfd
      421 klmslheedd pggnevidge reinfceipe ppdindsedq mefvptdfld qeqdiveete
      481 tasnkdnkee srdyssvele dgerseneke pcskekkpv
//
LOCUS       XP_034183326             599 aa            linear   INV 03-SEP-2025
DEFINITION  lamin Dm0 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183326
VERSION     XP_034183326.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327435.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..599
                     /product="lamin Dm0 isoform X1"
                     /calculated_mol_wt=68547
     Region          46..402
                     /region_name="Filament"
                     /note="Intermediate filament protein; pfam00038"
                     /db_xref="CDD:459643"
     Region          453..556
                     /region_name="LTD"
                     /note="Lamin Tail Domain; pfam00932"
                     /db_xref="CDD:460003"
     CDS             1..599
                     /gene="LOC117605755"
                     /coded_by="XM_034327435.2:198..1997"
                     /db_xref="GeneID:117605755"
ORIGIN      
        1 mstkaakkat atssstsgqs pqfststpig qrpgsplspt rysrlqekqd lqnlndrlac
       61 yidkvrhlea ensrltrevq ttqetitrev sniksmyehe lsdarklldd tareraklei
      121 dtkrlwdnne elkskldkkl mdlqvaernl llyetryndl qsqfnqsqae rkklaerere
      181 leqeveklkg lledtrkhlg eetlqrivle nniqslkedi sfkdqiyqqe ltetrtkrqi
      241 eiseidgrla eqyeaklqqs lqelrdqyea qmranreeie llyetkiknl tahaqrnsga
      301 aslaveelrq trsridtlnq kineleasnn alnarirdle nlrenektrh aeslasleae
      361 larirdemaq qlqeyqdlmd ikvaldleia ayrkllesee arlninpvqs sssvssgrtt
      421 psrhtplrgg krkrtllees eersstdysv tgtargdvev teadpqgrfv kltnkgnkei
      481 glsgwqiirk agsletvfkf hrtakieaga svmvwsadig atheppsniv mkgqkwftad
      541 tmtttllnne geematseck rqqlstslsr hrenlgfrps eelhhqqgdp qgeefcrlm
//
LOCUS       XP_034183327             594 aa            linear   INV 03-SEP-2025
DEFINITION  lamin Dm0 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183327
VERSION     XP_034183327.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327436.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..594
                     /product="lamin Dm0 isoform X2"
                     /calculated_mol_wt=68195
     Region          46..402
                     /region_name="Filament"
                     /note="Intermediate filament protein; pfam00038"
                     /db_xref="CDD:459643"
     Region          453..556
                     /region_name="LTD"
                     /note="Lamin Tail Domain; pfam00932"
                     /db_xref="CDD:460003"
     CDS             1..594
                     /gene="LOC117605755"
                     /coded_by="XM_034327436.2:199..1983"
                     /db_xref="GeneID:117605755"
ORIGIN      
        1 mstkaakkat atssstsgqs pqfststpig qrpgsplspt rysrlqekqd lqnlndrlac
       61 yidkvrhlea ensrltrevq ttqetitrev sniksmyehe lsdarklldd tareraklei
      121 dtkrlwdnne elkskldkkl mdlqvaernl llyetryndl qsqfnqsqae rkklaerere
      181 leqeveklkg lledtrkhlg eetlqrivle nniqslkedi sfkdqiyqqe ltetrtkrqi
      241 eiseidgrla eqyeaklqqs lqelrdqyea qmranreeie llyetkiknl tahaqrnsga
      301 aslaveelrq trsridtlnq kineleasnn alnarirdle nlrenektrh aeslasleae
      361 larirdemaq qlqeyqdlmd ikvaldleia ayrkllesee arlninpvqs sssvssgrtt
      421 psrhtplrgg krkrtllees eersstdysv tgtargdvev teadpqgrfv kltnkgnkei
      481 glsgwqiirk agsletvfkf hrtakieaga svmvwsadig atheppsniv mkgqkwftad
      541 tmtttllnne geematseck rqqlstslsr hrenlgfrps eelhhqqrry lypy
//
LOCUS       XP_076548099             459 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin [Osmia lignaria lignaria].
ACCESSION   XP_076548099
VERSION     XP_076548099.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691984.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..459
                     /product="neurotrimin"
                     /calculated_mol_wt=50953
     Region          52..142
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          61..65
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          74..78
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          123..128
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          135..138
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          146..238
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          165..169
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          178..182
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          203..207
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          217..222
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          231..234
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          242..334
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          259..263
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:143207"
     Region          272..276
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:143207"
     Region          302..306
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:143207"
     Region          316..321
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:143207"
     Region          329..332
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:143207"
     CDS             1..459
                     /gene="LOC117607272"
                     /coded_by="XM_076691984.1:82..1461"
                     /db_xref="GeneID:117607272"
ORIGIN      
        1 miservmrvl esiesrsrfl mmvlllstyt atvwtdnstg segpsftvpi tnvtvpmgre
       61 avlacvvanl stykvawlrv dtqtiltian hvitknhrig vthterktwh lhirevresd
      121 rgaymcqint dpmksqtgyl evvvppdild yststdmvvr egsnvtlrca atgspkpnit
      181 wrredgeail lengqevrsv egsiftitkv nrlqmgaylc iasngvpptv skrimlivhf
      241 tpmisiqnql vgaqegqkmt lechseafpk sinywtkenn eiiktgekyq qsvtdnayki
      301 qmkltilsve ksdygtykci sknslgetdg siklyhiptp ttqlkttttp sstveeveni
      361 qnsrqgplps mepiqnegtd tslpnilrnd dirlhgrass sdyndaentv tkgrknigge
      421 vrprridnta qsmssrgtst nflvqsrtai ciillsals
//
LOCUS       XP_034186692             415 aa            linear   INV 03-SEP-2025
DEFINITION  limbic system-associated membrane protein isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186692
VERSION     XP_034186692.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330801.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186692.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..415
                     /product="limbic system-associated membrane protein
                     isoform X1"
                     /calculated_mol_wt=46570
     Region          41..133
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          50..54
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          63..67
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          98..102
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          112..117
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..127
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          135..227
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          154..158
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          167..171
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          192..196
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          206..211
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          220..223
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          242..324
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          248..252
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          261..265
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          291..295
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          305..310
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          318..321
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..415
                     /gene="LOC117607271"
                     /coded_by="XM_034330801.2:90..1337"
                     /db_xref="GeneID:117607271"
ORIGIN      
        1 mcekrgmctr krqvpllvvt tllvgvrpel vdpnfkepia nvtasvgrea ilsclvqdla
       61 gykvawlrvd tqtiltiath vitknhriav shsdhrtwfl hirevresdr gwymcqintd
      121 pmksqigyle vvvppdildy qtstdmvvre gsnvtlrcta tgspepnivw kredgqkiil
      181 engtkvssig gtnfniskvt rlhmgsylci asngvpptvs krimlivhfp pmiwvqnqlv
      241 garegeqltl ecdseaypks inywtrdkde ivpqggkyep illdnaykvq mkltissvsp
      301 sdfgtykcvs rnslgdtdgt ikvyprisns sngniqykgk frhnsgndif egnqdmlker
      361 dlklrgrket sgnegddydd sgvateensf slifalvtli iyyhhhpqlr tlhpa
//
LOCUS       XP_034186693             372 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrimin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186693
VERSION     XP_034186693.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330802.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186693.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..372
                     /product="neurotrimin isoform X2"
                     /calculated_mol_wt=41896
     Region          <19..90
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          92..184
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          111..115
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          124..128
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          149..153
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          163..168
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          177..180
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          199..281
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          205..209
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          218..222
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          248..252
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          262..267
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          275..278
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..372
                     /gene="LOC117607271"
                     /coded_by="XM_034330802.2:569..1687"
                     /db_xref="GeneID:117607271"
ORIGIN      
        1 metvkpyprl apslatmyan vawlrvdtqt iltiathvit knhriavshs dhrtwflhir
       61 evresdrgwy mcqintdpmk sqigylevvv ppdildyqts tdmvvregsn vtlrctatgs
      121 pepnivwkre dgqkiileng tkvssiggtn fniskvtrlh mgsylciasn gvpptvskri
      181 mlivhfppmi wvqnqlvgar egeqltlecd seaypksiny wtrdkdeivp qggkyepill
      241 dnaykvqmkl tissvspsdf gtykcvsrns lgdtdgtikv yprisnssng niqykgkfrh
      301 nsgndifegn qdmlkerdlk lrgrketsgn egddyddsgv ateensfsli falvtliiyy
      361 hhhpqlrtlh pa
//
LOCUS       XP_034186701             816 aa            linear   INV 03-SEP-2025
DEFINITION  gcn5 acetyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_034186701
VERSION     XP_034186701.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330810.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186701.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..816
                     /product="gcn5 acetyltransferase"
                     /calculated_mol_wt=93903
     Region          52..301
                     /region_name="PCAF_N"
                     /note="PCAF (P300/CBP-associated factor) N-terminal
                     domain; pfam06466"
                     /db_xref="CDD:461923"
     Region          464..811
                     /region_name="COG5076"
                     /note="Transcription factor involved in chromatin
                     remodeling, contains bromodomain [Chromatin structure and
                     dynamics / Transcription]"
                     /db_xref="CDD:227408"
     Region          711..811
                     /region_name="Bromo_gcn5_like"
                     /note="Bromodomain; Gcn5_like subfamily. Gcn5p is a
                     histone acetyltransferase (HAT) which mediates acetylation
                     of histones at lysine residues; such acetylation is
                     generally correlated with the activation of transcription.
                     Bromodomains are 110 amino acid long...; cd05509"
                     /db_xref="CDD:99941"
     Site            order(736,741,744,783,787,793)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99941"
     CDS             1..816
                     /gene="Gcn5"
                     /coded_by="XM_034330810.2:232..2682"
                     /db_xref="GeneID:117607279"
ORIGIN      
        1 maseegnrna alqsldesgq svtstsqhag nsresttarp qeqssrqnnl qriqqrkqqm
       61 lnlpqmkkil kiatysacka edckcigwkn sqlltkspkn esqqpvinff dpckscthvl
      121 enhvshltnq seeeinkllc mvidadnifl glqkeedpdt kkvyfylykl lrkciqsmsk
      181 ptiegplgqp pferpsiaka vmnfvaykyg hlpqrdmqam cdmakmflhc lnhwnfdpls
      241 arrgtispee apaykinhtr wlvfchipaf cdslphydts lvfgrtfvqa vfkpvsrqll
      301 dkchserdkl apekrvivlt hfpkflsmle aeiyadnspi wdpdfkqvpl tylqtalesk
      361 anqgrktgef ekvmvppndk dnyttinisp gmkkihekrs hsegrsevkr rkneemfedl
      421 peetvaeiva tindpnymcg pdavfppnvp rdetakiees rkiiefhvvg nsltqpvskq
      481 tmlwliglhn vfshqlprmp keyisqlvfd pkhktlalik dgrpiggicf rmfstqgfte
      541 ivfcavtsqe qvkgygthlm nmlkdyhikn nilhfltfad efaigyfkkq gfskdiklpr
      601 smyqgyikdy egatlmhcel nakivyteft avirkqkeii kklihqrqqe iqkvhpgltc
      661 fkegvrgipv esipgiretg wksyaqtrtr gvakgtqgpe pmeaclditd slynalknvl
      721 nsvknhstaw pflkpvdknd vpdyydhiky pmdlktmtdr lkaryyvtrr lfiadmtrif
      781 tncrlynspd teyyrcanal ekyfqtrmke iglwdk
//
LOCUS       XP_076548100             816 aa            linear   INV 03-SEP-2025
DEFINITION  gcn5 acetyltransferase [Osmia lignaria lignaria].
ACCESSION   XP_076548100
VERSION     XP_076548100.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691985.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..816
                     /product="gcn5 acetyltransferase"
                     /calculated_mol_wt=93903
     Region          52..301
                     /region_name="PCAF_N"
                     /note="PCAF (P300/CBP-associated factor) N-terminal
                     domain; pfam06466"
                     /db_xref="CDD:461923"
     Region          464..811
                     /region_name="COG5076"
                     /note="Transcription factor involved in chromatin
                     remodeling, contains bromodomain [Chromatin structure and
                     dynamics / Transcription]"
                     /db_xref="CDD:227408"
     Region          711..811
                     /region_name="Bromo_gcn5_like"
                     /note="Bromodomain; Gcn5_like subfamily. Gcn5p is a
                     histone acetyltransferase (HAT) which mediates acetylation
                     of histones at lysine residues; such acetylation is
                     generally correlated with the activation of transcription.
                     Bromodomains are 110 amino acid long...; cd05509"
                     /db_xref="CDD:99941"
     Site            order(736,741,744,783,787,793)
                     /site_type="active"
                     /note="acetyllysine binding site [active]"
                     /db_xref="CDD:99941"
     CDS             1..816
                     /gene="Gcn5"
                     /coded_by="XM_076691985.1:4..2454"
                     /db_xref="GeneID:117607279"
ORIGIN      
        1 maseegnrna alqsldesgq svtstsqhag nsresttarp qeqssrqnnl qriqqrkqqm
       61 lnlpqmkkil kiatysacka edckcigwkn sqlltkspkn esqqpvinff dpckscthvl
      121 enhvshltnq seeeinkllc mvidadnifl glqkeedpdt kkvyfylykl lrkciqsmsk
      181 ptiegplgqp pferpsiaka vmnfvaykyg hlpqrdmqam cdmakmflhc lnhwnfdpls
      241 arrgtispee apaykinhtr wlvfchipaf cdslphydts lvfgrtfvqa vfkpvsrqll
      301 dkchserdkl apekrvivlt hfpkflsmle aeiyadnspi wdpdfkqvpl tylqtalesk
      361 anqgrktgef ekvmvppndk dnyttinisp gmkkihekrs hsegrsevkr rkneemfedl
      421 peetvaeiva tindpnymcg pdavfppnvp rdetakiees rkiiefhvvg nsltqpvskq
      481 tmlwliglhn vfshqlprmp keyisqlvfd pkhktlalik dgrpiggicf rmfstqgfte
      541 ivfcavtsqe qvkgygthlm nmlkdyhikn nilhfltfad efaigyfkkq gfskdiklpr
      601 smyqgyikdy egatlmhcel nakivyteft avirkqkeii kklihqrqqe iqkvhpgltc
      661 fkegvrgipv esipgiretg wksyaqtrtr gvakgtqgpe pmeaclditd slynalknvl
      721 nsvknhstaw pflkpvdknd vpdyydhiky pmdlktmtdr lkaryyvtrr lfiadmtrif
      781 tncrlynspd teyyrcanal ekyfqtrmke iglwdk
//
LOCUS       XP_034186703             364 aa            linear   INV 03-SEP-2025
DEFINITION  dnaJ homolog subfamily B member 12 [Osmia lignaria lignaria].
ACCESSION   XP_034186703
VERSION     XP_034186703.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330812.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..364
                     /product="dnaJ homolog subfamily B member 12"
                     /calculated_mol_wt=42495
     Region          101..>218
                     /region_name="DnaJ_bact"
                     /note="chaperone protein DnaJ; TIGR02349"
                     /db_xref="CDD:274090"
     Region          258..357
                     /region_name="DUF1977"
                     /note="Domain of unknown function (DUF1977); pfam09320"
                     /db_xref="CDD:462754"
     CDS             1..364
                     /gene="LOC117607280"
                     /coded_by="XM_034330812.2:135..1229"
                     /db_xref="GeneID:117607280"
ORIGIN      
        1 mdsnkdeaer cmelaerylr ekkyeeaekf vrkaqklypt kkaedvlaki tmlskqnqks
       61 eseptvrkrq tttkeathtq tsieyskeql eyvkrikkck dyyeilgvtk eatdsdikka
      121 ykklalqlhp dknkapgaae afkaignava iltdvekrkq ydmygpeeer mqnvqtrqnh
      181 ahynytrgfe tditaeelfn mffgggfpqq efytrrsgrw arqadaqaqh ahsqqtngyt
      241 tflqmlpvll lilltmmssf fisdpvyslh snakysvlrt tqglkvpyyv kenfhteyqg
      301 slrrleisve eeylnnlrha cfreksyrdt mmwkarnfgd qdlffkakni elpsckrvqd
      361 lqga
//
LOCUS       XP_034186725             430 aa            linear   INV 03-SEP-2025
DEFINITION  inositol phosphate kinase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034186725
VERSION     XP_034186725.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330834.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186725.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="inositol phosphate kinase 2"
                     /calculated_mol_wt=49251
     Region          118..>256
                     /region_name="IPK"
                     /note="Inositol polyphosphate kinase; pfam03770"
                     /db_xref="CDD:397715"
     CDS             1..430
                     /gene="Ipk2"
                     /coded_by="XM_034330834.2:521..1813"
                     /db_xref="GeneID:117607292"
ORIGIN      
        1 mttvesqtdk tlshldkpsn tevaeipsdr glpvgisple srvsghpfdd erqmigmlrr
       61 sdgrvfkpvv kpmlgkreia fyenlqvsqd pmllqlrnyv pryygttdfq vfgrritflt
      121 lkditdgmae pcvmdikigk rtwdplasse kkakeelkya eskrtygfci tgfqvyctss
      181 gqlrkfgkhy gktldakgvv ealkiflnis perppcrqli vkllsflwki llffrtqrlf
      241 rfysssllva ydakrlrhyh rlgnkdtgcl pktftssnsh askssvfnta gksiansvet
      301 srnrtmervh fikrsislss eydkiekert lnrsgscspd frvsrlcrth syvnnfdddi
      361 vkikedyddl lneltsssee kqnwvrinmi dfthvfpaed ptaldmnyle gienliklve
      421 mflvskdqaa
//
LOCUS       XP_076548101             430 aa            linear   INV 03-SEP-2025
DEFINITION  inositol phosphate kinase 2 [Osmia lignaria lignaria].
ACCESSION   XP_076548101
VERSION     XP_076548101.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691986.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="inositol phosphate kinase 2"
                     /calculated_mol_wt=49251
     Region          118..>256
                     /region_name="IPK"
                     /note="Inositol polyphosphate kinase; pfam03770"
                     /db_xref="CDD:397715"
     CDS             1..430
                     /gene="Ipk2"
                     /coded_by="XM_076691986.1:713..2005"
                     /db_xref="GeneID:117607292"
ORIGIN      
        1 mttvesqtdk tlshldkpsn tevaeipsdr glpvgisple srvsghpfdd erqmigmlrr
       61 sdgrvfkpvv kpmlgkreia fyenlqvsqd pmllqlrnyv pryygttdfq vfgrritflt
      121 lkditdgmae pcvmdikigk rtwdplasse kkakeelkya eskrtygfci tgfqvyctss
      181 gqlrkfgkhy gktldakgvv ealkiflnis perppcrqli vkllsflwki llffrtqrlf
      241 rfysssllva ydakrlrhyh rlgnkdtgcl pktftssnsh askssvfnta gksiansvet
      301 srnrtmervh fikrsislss eydkiekert lnrsgscspd frvsrlcrth syvnnfdddi
      361 vkikedyddl lneltsssee kqnwvrinmi dfthvfpaed ptaldmnyle gienliklve
      421 mflvskdqaa
//
LOCUS       XP_034186726             430 aa            linear   INV 03-SEP-2025
DEFINITION  inositol phosphate kinase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034186726
VERSION     XP_034186726.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330835.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186726.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="inositol phosphate kinase 2"
                     /calculated_mol_wt=49251
     Region          118..>256
                     /region_name="IPK"
                     /note="Inositol polyphosphate kinase; pfam03770"
                     /db_xref="CDD:397715"
     CDS             1..430
                     /gene="Ipk2"
                     /coded_by="XM_034330835.2:443..1735"
                     /db_xref="GeneID:117607292"
ORIGIN      
        1 mttvesqtdk tlshldkpsn tevaeipsdr glpvgisple srvsghpfdd erqmigmlrr
       61 sdgrvfkpvv kpmlgkreia fyenlqvsqd pmllqlrnyv pryygttdfq vfgrritflt
      121 lkditdgmae pcvmdikigk rtwdplasse kkakeelkya eskrtygfci tgfqvyctss
      181 gqlrkfgkhy gktldakgvv ealkiflnis perppcrqli vkllsflwki llffrtqrlf
      241 rfysssllva ydakrlrhyh rlgnkdtgcl pktftssnsh askssvfnta gksiansvet
      301 srnrtmervh fikrsislss eydkiekert lnrsgscspd frvsrlcrth syvnnfdddi
      361 vkikedyddl lneltsssee kqnwvrinmi dfthvfpaed ptaldmnyle gienliklve
      421 mflvskdqaa
//
LOCUS       XP_034186728             205 aa            linear   INV 03-SEP-2025
DEFINITION  meiotic nuclear division protein 1 homolog [Osmia lignaria
            lignaria].
ACCESSION   XP_034186728
VERSION     XP_034186728.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330837.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186728.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..205
                     /product="meiotic nuclear division protein 1 homolog"
                     /calculated_mol_wt=23759
     Region          5..203
                     /region_name="COG5124"
                     /note="Protein predicted to be involved in meiotic
                     recombination [Cell division and chromosome partitioning /
                     General function prediction only]"
                     /db_xref="CDD:227453"
     CDS             1..205
                     /gene="LOC117607293"
                     /coded_by="XM_034330837.2:14..631"
                     /db_xref="GeneID:117607293"
ORIGIN      
        1 mskrkgisld ekrarmlqif yekkefytlk elekiapkek giivqsvkdv lqtlvddglv
       61 rsekigtsvy fwtfpgenit aieqriseas kkiveaefkl qklkeacvke qigkedteer
      121 qillheleel kakevqlkkq iakfsdadpe aiaklvekaq eykdaanvwt dnifaiqswc
      181 krkfdiseel lnkqfnipnd ldyke
//
LOCUS       XP_034186730              73 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor B5 [Osmia lignaria lignaria].
ACCESSION   XP_034186730
VERSION     XP_034186730.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330839.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..73
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..73
                     /product="transcription factor B5"
                     /calculated_mol_wt=8146
     Region          1..68
                     /region_name="Tfb5"
                     /note="Transcription factor TFIIH complex subunit Tfb5;
                     pfam06331"
                     /db_xref="CDD:428891"
     CDS             1..73
                     /gene="Tfb5"
                     /coded_by="XM_034330839.2:106..327"
                     /db_xref="GeneID:117607295"
ORIGIN      
        1 mvnvtkgilv ecdaamkqfl lhldetfalg rkfiiqdlde rhlfisvdil dtlqakvddl
       61 mdqisfplad kgi
//
LOCUS       XP_034186729             154 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger HIT domain-containing protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186729
VERSION     XP_034186729.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330838.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..154
                     /product="zinc finger HIT domain-containing protein 1"
                     /calculated_mol_wt=17691
     Region          13..>77
                     /region_name="DUF1308"
                     /note="Protein of unknown function (DUF1308); pfam07000"
                     /db_xref="CDD:399767"
     Region          58..>135
                     /region_name="YL1_C"
                     /note="YL1 nuclear protein C-terminal domain; cl02154"
                     /db_xref="CDD:470478"
     Region          108..150
                     /region_name="zf-HIT_ZNHIT1_like"
                     /note="HIT zinc finger found in zinc finger HIT
                     domain-containing protein 1 (ZNHIT1) and similar proteins;
                     cd21437"
                     /db_xref="CDD:467791"
     Site            order(112..116,119..122,124..127,129,137..138)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:467791"
     Site            order(117,120,128,131,136,140,144,149)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:467791"
     CDS             1..154
                     /gene="LOC117607294"
                     /coded_by="XM_034330838.2:7..471"
                     /db_xref="GeneID:117607294"
ORIGIN      
        1 maaresgrik dayqkrmlde aarkrrqkka lealeqdnfh ddphadlvms kkvpkfqetl
       61 dnrggrrkkt rsaeyykqrf rktfaqlvee dlninpnppn yasaqappsr fperqfcavc
      121 gfpsnytcip cgarycsmkc lgthldtrcl kwta
//
LOCUS       XP_034182982             857 aa            linear   INV 03-SEP-2025
DEFINITION  tetratricopeptide repeat domain 7 [Osmia lignaria lignaria].
ACCESSION   XP_034182982
VERSION     XP_034182982.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327091.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..857
                     /product="tetratricopeptide repeat domain 7"
                     /calculated_mol_wt=97243
     Region          1..400
                     /region_name="TTC7_N"
                     /note="Tetratricopeptide repeat protein 7 N-terminal;
                     pfam19440"
                     /db_xref="CDD:466084"
     Region          <399..626
                     /region_name="LapB"
                     /note="Lipopolysaccharide biosynthesis regulator
                     YciM/LapB, contains six TPR domains and a C-terminal
                     metal-binding domain [Cell wall/membrane/envelope
                     biogenesis]; COG2956"
                     /db_xref="CDD:442196"
     Region          411..441
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          446..475
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          481..525
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          523..>845
                     /region_name="PEP_TPR_lipo"
                     /note="putative PEP-CTERM system TPR-repeat lipoprotein;
                     TIGR02917"
                     /db_xref="CDD:274350"
     Region          530..560
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          565..593
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          717..>845
                     /region_name="TPR"
                     /note="Tetratricopeptide (TPR) repeat [General function
                     prediction only]; COG0457"
                     /db_xref="CDD:440225"
     Region          745..771
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Site            order(747..748,751..752,754,778,781..782,785..786,
                     788..789,812,815..816,819..820,823)
                     /site_type="other"
                     /note="putative protein binding surface [polypeptide
                     binding]"
                     /db_xref="CDD:276809"
     Region          776..806
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     Region          811..839
                     /region_name="TPR repeat"
                     /note="TPR repeat [structural motif]"
                     /db_xref="CDD:276809"
     CDS             1..857
                     /gene="Ttc7"
                     /coded_by="XM_034327091.2:155..2728"
                     /db_xref="GeneID:117605585"
ORIGIN      
        1 mtskkghtlr ieseidknre egnwkkviel aehlktqyps neclanflcg egrlesfleq
       61 tppvdanigk arnglaetrr ylllaanekd kqalvvldah lllgkvhyam gmyeealnhy
      121 qqaelhalte kplpcrslri iaesyaikdt ekhlscrrlr iiaesyaikg lcleklppys
      181 kskykiaewq eqiikcyeia gdltlvylqe qdklamqhqn gtstvnsnna gtnssqssfc
      241 stkhigpile ttlqrapily iqtgniqgai nryrevlsav estatqslrv mltrqlaevl
      301 vrgisgaeyk ppepssdttd spwkpkkylg pnmfvprney eetillllis eamavrdavl
      361 sqspefkear ihafenatav ydlltvvvvr wsqvdllyes feramkfshe eahvwtqcal
      421 clismgrymh ayrvlrvvar ltpqkvmpcl liarlcyeql nmvregiews qkalqretkn
      481 sqgmqsrchl yigighsila tntivkmdkt yhtktalecf qkaqqcdpnd hlaeyylahe
      541 yainrqinda mihvkialnl raehipslhl lvlllsahkq ysealhlvns vleeypdnln
      601 flyvkahlel rsiggeqalf tikhmlllwk hlyedqtntn cneqhsekrs etrsvfqlya
      661 semsdkdsss vhaqslaasr veqalsevas slssftpkpg pqrawllqlq vwllltevyl
      721 vldqpngavl slqeatnifp lshhimytrg llheykleym eakqcyqnav sinpshiksl
      781 qhlgliyhyl gsqrlaektl rdaakidpns hqtwynlgkv leslgeveaa sdcmatalev
      841 ettnpilpvl sipvtfe
//
LOCUS       XP_034182987             428 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182987
VERSION     XP_034182987.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327096.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..428
                     /product="KICSTOR complex protein kaptin isoform X1"
                     /calculated_mol_wt=48705
     Region          337..395
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..428
                     /gene="LOC117605586"
                     /coded_by="XM_034327096.2:287..1573"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mrtdhmcgke rimnnltdah wfpltsqgni ysmtklcsns gynkvlvasl krkiysceyh
       61 lmpnlhlkpl vkellftyip sgteiisida ynksdggdgf vigitilkas tdtsveryln
      121 iytegaidge gdegssieai aqncltvelp ytpyhlyhti lpqqnshsev vwllsgsdyk
      181 ihmiredkln hgysessiek yfpelhdiqg iaiwiniyyy dnynwrltvi gcecglviva
      241 ivnvlelkvc kawklrydrp issvcifpqq nnvtkpsfln srgpkcslsn etklnvlivn
      301 tsnavvfmdi lnngmdedit lngsetsdci lcsctadinm dgrneillgt ygqevlifcf
      361 inntweliir klfdapvhsi cymditndgm kelivltqrg vhilqhnimd vkdkwkkryr
      421 kmlkdnrt
//
LOCUS       XP_034182988             425 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182988
VERSION     XP_034182988.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327097.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..425
                     /product="KICSTOR complex protein kaptin isoform X2"
                     /calculated_mol_wt=48292
     Region          334..392
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..425
                     /gene="LOC117605586"
                     /coded_by="XM_034327097.2:287..1564"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mrtdhmcgim nnltdahwfp ltsqgniysm tklcsnsgyn kvlvaslkrk iysceyhlmp
       61 nlhlkplvke llftyipsgt eiisidaynk sdggdgfvig itilkastdt sverylniyt
      121 egaidgegde gssieaiaqn cltvelpytp yhlyhtilpq qnshsevvwl lsgsdykihm
      181 iredklnhgy sessiekyfp elhdiqgiai winiyyydny nwrltvigce cglvivaivn
      241 vlelkvckaw klrydrpiss vcifpqqnnv tkpsflnsrg pkcslsnetk lnvlivntsn
      301 avvfmdilnn gmdeditlng setsdcilcs ctadinmdgr neillgtygq evlifcfinn
      361 tweliirklf dapvhsicym ditndgmkel ivltqrgvhi lqhnimdvkd kwkkryrkml
      421 kdnrt
//
LOCUS       XP_034182989             416 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182989
VERSION     XP_034182989.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327098.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="KICSTOR complex protein kaptin isoform X3"
                     /calculated_mol_wt=47246
     Region          325..383
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..416
                     /gene="LOC117605586"
                     /coded_by="XM_034327098.2:287..1537"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mnnltdahwf pltsqgniys mtklcsnsgy nkvlvaslkr kiysceyhlm pnlhlkplvk
       61 ellftyipsg teiisidayn ksdggdgfvi gitilkastd tsverylniy tegaidgegd
      121 egssieaiaq ncltvelpyt pyhlyhtilp qqnshsevvw llsgsdykih miredklnhg
      181 ysessiekyf pelhdiqgia iwiniyyydn ynwrltvigc ecglvivaiv nvlelkvcka
      241 wklrydrpis svcifpqqnn vtkpsflnsr gpkcslsnet klnvlivnts navvfmdiln
      301 ngmdeditln gsetsdcilc sctadinmdg rneillgtyg qevlifcfin ntweliirkl
      361 fdapvhsicy mditndgmke livltqrgvh ilqhnimdvk dkwkkryrkm lkdnrt
//
LOCUS       XP_034182990             416 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182990
VERSION     XP_034182990.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327099.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="KICSTOR complex protein kaptin isoform X3"
                     /calculated_mol_wt=47246
     Region          325..383
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..416
                     /gene="LOC117605586"
                     /coded_by="XM_034327099.2:166..1416"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mnnltdahwf pltsqgniys mtklcsnsgy nkvlvaslkr kiysceyhlm pnlhlkplvk
       61 ellftyipsg teiisidayn ksdggdgfvi gitilkastd tsverylniy tegaidgegd
      121 egssieaiaq ncltvelpyt pyhlyhtilp qqnshsevvw llsgsdykih miredklnhg
      181 ysessiekyf pelhdiqgia iwiniyyydn ynwrltvigc ecglvivaiv nvlelkvcka
      241 wklrydrpis svcifpqqnn vtkpsflnsr gpkcslsnet klnvlivnts navvfmdiln
      301 ngmdeditln gsetsdcilc sctadinmdg rneillgtyg qevlifcfin ntweliirkl
      361 fdapvhsicy mditndgmke livltqrgvh ilqhnimdvk dkwkkryrkm lkdnrt
//
LOCUS       XP_034182991             416 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182991
VERSION     XP_034182991.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327100.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="KICSTOR complex protein kaptin isoform X3"
                     /calculated_mol_wt=47246
     Region          325..383
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..416
                     /gene="LOC117605586"
                     /coded_by="XM_034327100.2:309..1559"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mnnltdahwf pltsqgniys mtklcsnsgy nkvlvaslkr kiysceyhlm pnlhlkplvk
       61 ellftyipsg teiisidayn ksdggdgfvi gitilkastd tsverylniy tegaidgegd
      121 egssieaiaq ncltvelpyt pyhlyhtilp qqnshsevvw llsgsdykih miredklnhg
      181 ysessiekyf pelhdiqgia iwiniyyydn ynwrltvigc ecglvivaiv nvlelkvcka
      241 wklrydrpis svcifpqqnn vtkpsflnsr gpkcslsnet klnvlivnts navvfmdiln
      301 ngmdeditln gsetsdcilc sctadinmdg rneillgtyg qevlifcfin ntweliirkl
      361 fdapvhsicy mditndgmke livltqrgvh ilqhnimdvk dkwkkryrkm lkdnrt
//
LOCUS       XP_034182992             416 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182992
VERSION     XP_034182992.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327101.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="KICSTOR complex protein kaptin isoform X3"
                     /calculated_mol_wt=47246
     Region          325..383
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..416
                     /gene="LOC117605586"
                     /coded_by="XM_034327101.2:197..1447"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mnnltdahwf pltsqgniys mtklcsnsgy nkvlvaslkr kiysceyhlm pnlhlkplvk
       61 ellftyipsg teiisidayn ksdggdgfvi gitilkastd tsverylniy tegaidgegd
      121 egssieaiaq ncltvelpyt pyhlyhtilp qqnshsevvw llsgsdykih miredklnhg
      181 ysessiekyf pelhdiqgia iwiniyyydn ynwrltvigc ecglvivaiv nvlelkvcka
      241 wklrydrpis svcifpqqnn vtkpsflnsr gpkcslsnet klnvlivnts navvfmdiln
      301 ngmdeditln gsetsdcilc sctadinmdg rneillgtyg qevlifcfin ntweliirkl
      361 fdapvhsicy mditndgmke livltqrgvh ilqhnimdvk dkwkkryrkm lkdnrt
//
LOCUS       XP_034182993             416 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182993
VERSION     XP_034182993.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327102.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="KICSTOR complex protein kaptin isoform X3"
                     /calculated_mol_wt=47246
     Region          325..383
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..416
                     /gene="LOC117605586"
                     /coded_by="XM_034327102.2:205..1455"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mnnltdahwf pltsqgniys mtklcsnsgy nkvlvaslkr kiysceyhlm pnlhlkplvk
       61 ellftyipsg teiisidayn ksdggdgfvi gitilkastd tsverylniy tegaidgegd
      121 egssieaiaq ncltvelpyt pyhlyhtilp qqnshsevvw llsgsdykih miredklnhg
      181 ysessiekyf pelhdiqgia iwiniyyydn ynwrltvigc ecglvivaiv nvlelkvcka
      241 wklrydrpis svcifpqqnn vtkpsflnsr gpkcslsnet klnvlivnts navvfmdiln
      301 ngmdeditln gsetsdcilc sctadinmdg rneillgtyg qevlifcfin ntweliirkl
      361 fdapvhsicy mditndgmke livltqrgvh ilqhnimdvk dkwkkryrkm lkdnrt
//
LOCUS       XP_076548102             396 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548102
VERSION     XP_076548102.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691987.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="KICSTOR complex protein kaptin isoform X4"
                     /calculated_mol_wt=44955
     Region          305..363
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..396
                     /gene="LOC117605586"
                     /coded_by="XM_076691987.1:326..1516"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mtklcsnsgy nkvlvaslkr kiysceyhlm pnlhlkplvk ellftyipsg teiisidayn
       61 ksdggdgfvi gitilkastd tsverylniy tegaidgegd egssieaiaq ncltvelpyt
      121 pyhlyhtilp qqnshsevvw llsgsdykih miredklnhg ysessiekyf pelhdiqgia
      181 iwiniyyydn ynwrltvigc ecglvivaiv nvlelkvcka wklrydrpis svcifpqqnn
      241 vtkpsflnsr gpkcslsnet klnvlivnts navvfmdiln ngmdeditln gsetsdcilc
      301 sctadinmdg rneillgtyg qevlifcfin ntweliirkl fdapvhsicy mditndgmke
      361 livltqrgvh ilqhnimdvk dkwkkryrkm lkdnrt
//
LOCUS       XP_034182994             396 aa            linear   INV 03-SEP-2025
DEFINITION  KICSTOR complex protein kaptin isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182994
VERSION     XP_034182994.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327103.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="KICSTOR complex protein kaptin isoform X4"
                     /calculated_mol_wt=44955
     Region          305..363
                     /region_name="FG-GAP_3"
                     /note="FG-GAP-like repeat; pfam13517"
                     /db_xref="CDD:433275"
     CDS             1..396
                     /gene="LOC117605586"
                     /coded_by="XM_034327103.2:348..1538"
                     /db_xref="GeneID:117605586"
ORIGIN      
        1 mtklcsnsgy nkvlvaslkr kiysceyhlm pnlhlkplvk ellftyipsg teiisidayn
       61 ksdggdgfvi gitilkastd tsverylniy tegaidgegd egssieaiaq ncltvelpyt
      121 pyhlyhtilp qqnshsevvw llsgsdykih miredklnhg ysessiekyf pelhdiqgia
      181 iwiniyyydn ynwrltvigc ecglvivaiv nvlelkvcka wklrydrpis svcifpqqnn
      241 vtkpsflnsr gpkcslsnet klnvlivnts navvfmdiln ngmdeditln gsetsdcilc
      301 sctadinmdg rneillgtyg qevlifcfin ntweliirkl fdapvhsicy mditndgmke
      361 livltqrgvh ilqhnimdvk dkwkkryrkm lkdnrt
//
LOCUS       XP_034190742             231 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609021 [Osmia lignaria lignaria].
ACCESSION   XP_034190742
VERSION     XP_034190742.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334851.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..231
                     /product="uncharacterized protein LOC117609021"
                     /calculated_mol_wt=26526
     CDS             1..231
                     /gene="LOC117609021"
                     /coded_by="XM_034334851.2:438..1133"
                     /db_xref="GeneID:117609021"
ORIGIN      
        1 msfwsrrrfr itplralaaf flmvmlfwys lsrqetprqp csvpeeftek lhelayrahl
       61 vlaklgivhf lclgglwgqv rigralpwar kvelclvdtl rddvlitkaf revglsatyl
      121 haagvyriqe hevgedapkv evvvfeedav trmvrrvgwt rrvlppdcef spslqcfppq
      181 lvipplpakq fggrlmpvpr egielqkyhy pndwwlevkp tdctepqgtn a
//
LOCUS       XP_034190744             231 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609021 [Osmia lignaria lignaria].
ACCESSION   XP_034190744
VERSION     XP_034190744.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..231
                     /product="uncharacterized protein LOC117609021"
                     /calculated_mol_wt=26526
     CDS             1..231
                     /gene="LOC117609021"
                     /coded_by="XM_034334853.2:357..1052"
                     /db_xref="GeneID:117609021"
ORIGIN      
        1 msfwsrrrfr itplralaaf flmvmlfwys lsrqetprqp csvpeeftek lhelayrahl
       61 vlaklgivhf lclgglwgqv rigralpwar kvelclvdtl rddvlitkaf revglsatyl
      121 haagvyriqe hevgedapkv evvvfeedav trmvrrvgwt rrvlppdcef spslqcfppq
      181 lvipplpakq fggrlmpvpr egielqkyhy pndwwlevkp tdctepqgtn a
//
LOCUS       XP_034182995             481 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein no ocelli [Osmia lignaria lignaria].
ACCESSION   XP_034182995
VERSION     XP_034182995.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327104.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="zinc finger protein no ocelli"
                     /calculated_mol_wt=49709
     Region          16..>59
                     /region_name="SP1-4_N"
                     /note="N-terminal domain of transcription factor
                     Specificity Proteins (SP) 1-4; cl41773"
                     /db_xref="CDD:425404"
     Region          356..384
                     /region_name="zf-C2H2"
                     /note="Zinc finger, C2H2 type; pfam00096"
                     /db_xref="CDD:395048"
     Region          358..384
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..481
                     /gene="noc"
                     /coded_by="XM_034327104.2:575..2020"
                     /db_xref="GeneID:117605587"
ORIGIN      
        1 mvvleeggml ttghnqylqp dylsplpttl dakksplall aqtcsqigad spskplispl
       61 dktpksmngg anaksppaak lerstrssps dgkslafkpy etnvvskkst desrpaskas
      121 vhsvsgqdsv sasdnnhtek kssgnrtpvg rksaspasqa tvsttpladr ktpvdrektd
      181 gsprnsnnga spiirsgmei lsgghakdsn lgaykpglag fsgnplccpp glaenpafrp
      241 pftgaspfsh hhaaaaallg ypsttptggn pylsyarvkt paggealvpv ckdpyctgcq
      301 ysmhsaqlmm gtgtcpsgct qcdhqkygla malsslgpma ppslsypstl tggrpyvcnw
      361 iagdsycgkr fttseellqh lrshtsltgg dhasssaall snphphplls psaalhragg
      421 gsyptpslsp lsaryhpygk pptgplpasl aaspysafna lgpyyspyai ygqrigaavh
      481 p
//
LOCUS       XP_034182981            1197 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor-related protein complex 3, delta 1 subunit-like garnet
            [Osmia lignaria lignaria].
ACCESSION   XP_034182981
VERSION     XP_034182981.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1197
                     /product="adaptor-related protein complex 3, delta 1
                     subunit-like garnet"
                     /calculated_mol_wt=136046
     Region          32..578
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          659..791
                     /region_name="AP3D1"
                     /note="AP-3 complex subunit delta-1; pfam06375"
                     /db_xref="CDD:461889"
     CDS             1..1197
                     /gene="garnet"
                     /coded_by="XM_034327090.2:242..3835"
                     /db_xref="GeneID:117605584"
ORIGIN      
        1 malrkvkgnf ermfdknltd lvrgirnnke neakyiaqci eeikqelrqd nvavkanava
       61 kltylqmlgy diswagfnii evmssakfty krigylaasq sfhadtellm lttnmirkdl
      121 nsqnqydagl alsglscfit sdlardlvnd imtlltstkp ylrkkavlmm ykvflrfpea
      181 lrpafprlke kledpdsgvq saavnvvcel arknpknyls lapvffklmt tstnnwmlik
      241 iiklfgaltp leprlgkkli epltnlihst samsllyeci ntviavlisi ssgmpnhsds
      301 iqlcvqklri liedsdqnlk ylgllamski lkthpksvqa hkdlimqcld dkdesirlra
      361 ldllygmvsk knlmeivrkl mvhmdkaegt ayrdellski iqicsqnnyq fvtyfewyis
      421 vlveltrmeg tkhgplvatq lldvairvqa irkyavqqca lllensyllt gqpratmsev
      481 lyaaawicge fsseledpla tlqsmlrsqa sslpghiqav yvhnilklat atlykaekdg
      541 dtetmeqifa lkdkiaafvc sgdlevqers ssalvllecl kenpglveel meafegelnp
      601 vapkaqrkvp ipedldldsw indppsessd sedldmndif iktdktsdsy skreliepsa
      661 eelqkrrdar kleqennphy lkgtfknsas yhnsssiyee fenipvaeld ipvslkitnn
      721 hkrykvhsrk kkkskknkka tsedeqddsy pvqvvntgvg elppgaelsd sddydqvdln
      781 dphralnidl dlplrdderl pvlehrvien nmihgsgesk gkkkeqgykk skkkvkeake
      841 rrhdnqlelt nmwlednass eklqspivne ytevfsekes eqkdtkrkks kshdkhkktd
      901 edskhrkskk skskkdkksa dyeetagist pskeilpdlk ynftnsadst tlvqptnsye
      961 elaknkvlsl tyelkqisde sdnlvatili tnncqklvkd lvfdvpdtpt lrlmkdvgde
     1021 fgiklpfqlp pessketqfi ilisddtfaq tlrgtltyml enkentvqek ldfsmhlrcs
     1081 kfmvgylphr diltellksr qficknrrlr klvsfsenfd vilnaicckc nlilveqiyd
     1141 taslyghslk ghhvclllky dkfkhtflle gkgdsdtlls evadevleii dahqmik
//
LOCUS       XP_034182873             324 aa            linear   INV 03-SEP-2025
DEFINITION  calumenin B scf [Osmia lignaria lignaria].
ACCESSION   XP_034182873
VERSION     XP_034182873.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326982.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..324
                     /product="calumenin B scf"
                     /calculated_mol_wt=37697
     Region          41..308
                     /region_name="EFh_CREC_Calumenin_like"
                     /note="EF-hand, calcium binding motif, found in calumenin,
                     reticulocalbin-1 (RCN-1), reticulocalbin-3 (RCN-3), and
                     similar proteins; cd16226"
                     /db_xref="CDD:320024"
     Region          41..69
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320024"
     Region          75..104
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320024"
     Region          111..140
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320024"
     Region          164..193
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320024"
     Region          201..230
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320024"
     Region          242..271
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320024"
     Region          278..308
                     /region_name="EF-hand motif"
                     /note="EF-hand motif [structural motif]"
                     /db_xref="CDD:320024"
     CDS             1..324
                     /gene="scf"
                     /coded_by="XM_034326982.2:318..1292"
                     /db_xref="GeneID:117605545"
ORIGIN      
        1 mqefmllqil igfsvlatai skpenehktr vvnkepseee hyvhsqhnpa ydheaflgee
       61 sktfdqltpe estrrlgiiv dkidkdkdgy vtgeelkdwi lytqrryird nverqwkshn
      121 pegkeklpwt eylamvygdm deqeaenhek skentfsyva mlkkdrrrws tadldgddal
      181 tkeefaaflh aeeadhmkdv vvletmedid kdgdgkisls eyigdmfegp egeeepewvk
      241 nekeqfsmyr dkdgdgflnf eevktwiipa dfdhaeaesr hlifeadtda dqkltkdeil
      301 ekydifvgsq atdfgdaltr hdef
//
LOCUS       XP_034182875             241 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 2A [Osmia lignaria lignaria].
ACCESSION   XP_034182875
VERSION     XP_034182875.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326984.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="tetraspanin 2A"
                     /calculated_mol_wt=26816
     Region          16..227
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     CDS             1..241
                     /gene="Tsp2A"
                     /coded_by="XM_034326984.2:136..861"
                     /db_xref="GeneID:117605547"
ORIGIN      
        1 magkngpkle qriqcikfti fclnaiiwll gsalfglcmw lrldpsfeew vdflnmyefy
       61 iggyillaas ifiiiitllg cavalmehil glyvyvglql asyvlglvgt ailldystyd
      121 skiqplirqs mtdlisrshd gratyilqli qesigccgad gpmdyltlrk plptecrnsv
      181 tgnayfhgcv eeiswflear sgwvaglslt lcllhvviaa lsltliraik keeqlvvsyk
      241 r
//
LOCUS       XP_076548103             241 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 2A [Osmia lignaria lignaria].
ACCESSION   XP_076548103
VERSION     XP_076548103.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691988.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..241
                     /product="tetraspanin 2A"
                     /calculated_mol_wt=26816
     Region          16..227
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     CDS             1..241
                     /gene="Tsp2A"
                     /coded_by="XM_076691988.1:178..903"
                     /db_xref="GeneID:117605547"
ORIGIN      
        1 magkngpkle qriqcikfti fclnaiiwll gsalfglcmw lrldpsfeew vdflnmyefy
       61 iggyillaas ifiiiitllg cavalmehil glyvyvglql asyvlglvgt ailldystyd
      121 skiqplirqs mtdlisrshd gratyilqli qesigccgad gpmdyltlrk plptecrnsv
      181 tgnayfhgcv eeiswflear sgwvaglslt lcllhvviaa lsltliraik keeqlvvsyk
      241 r
//
LOCUS       XP_034182879             169 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605549 [Osmia lignaria lignaria].
ACCESSION   XP_034182879
VERSION     XP_034182879.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326988.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..169
                     /product="uncharacterized protein LOC117605549"
                     /calculated_mol_wt=18890
     CDS             1..169
                     /gene="LOC117605549"
                     /coded_by="XM_034326988.2:83..592"
                     /db_xref="GeneID:117605549"
ORIGIN      
        1 miniilcatl iicaeslsla etdmpkcfqy twiapafnik pdhfnctklt dfpcieppii
       61 tdkppdphem annesmlctl tssnvcvkyt yrynggiart ssfcgkvied dlipvtsgcy
      121 qqrvnshdre vcacqtrrge dpcnmsiqtk ysiylivtvl ilvviydlc
//
LOCUS       XP_034183159             629 aa            linear   INV 03-SEP-2025
DEFINITION  beta-galactosidase [Osmia lignaria lignaria].
ACCESSION   XP_034183159
VERSION     XP_034183159.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327268.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="beta-galactosidase"
                     /calculated_mol_wt=70704
     Region          40..356
                     /region_name="Glyco_hydro_35"
                     /note="Glycosyl hydrolases family 35; pfam01301"
                     /db_xref="CDD:396048"
     Region          <539..606
                     /region_name="BetaGal_dom4_5"
                     /note="Beta-galactosidase jelly roll domain; pfam13364"
                     /db_xref="CDD:463857"
     CDS             1..629
                     /gene="LOC117605673"
                     /coded_by="XM_034327268.2:313..2202"
                     /db_xref="GeneID:117605673"
ORIGIN      
        1 mwflllvlaa tgvmgqavnv hvendtqlkf gfevdyvndq flldgkpfry vsgsfhyfrt
       61 prqywrdrlr kiraaglnav styvewslhq ptedkwewtg dadllkfldi aqeedllvll
      121 rpgpyicaer dfgglpywli prvssgklrv ndpqymhyae iylnevlerv kpylrgnggp
      181 iimvqveney gsfacdtqym srlrdimkek ietkallytt ngandvmlsc gsvpnvytti
      241 dfgtstnvkk tfetlrvyqs qgplvnsefy pgwlthweep fqrvksgavk ktlnemlsmg
      301 asvniymfyg gtnfgytaga nggpdayspq ltsydydapl teagdptpky feirnvvsqy
      361 lplpntsvpt vspkgdygpi fltpvlklle pigrqllgtv pveslvpltf etlglshwlv
      421 lyetdlvtnp sdpavlhamv rdralvyvdd hlvgtlsrtn kvydivslee pygqklkllv
      481 enqgrlnygs glydykgisn vtlngvplgp wrmtgypldd isplndlkei tiksgtllng
      541 pvilrgnfsv sgqpmdtfln tvgwgkgvaf vnghnlgryw plpgpqitly vpapflktge
      601 nelivveley vpsnrkmklq iqpilhnkf
//
LOCUS       XP_076548104            1001 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548104
VERSION     XP_076548104.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691989.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1001
                     /product="anoctamin 1 isoform X1"
                     /calculated_mol_wt=116164
     Region          122..342
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          345..925
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..1001
                     /gene="subdued"
                     /coded_by="XM_076691989.1:355..3360"
                     /db_xref="GeneID:117607258"
ORIGIN      
        1 mkeddeevsv pwiqdhrnsr sdrsrsdvsa yqsiqsnlna gsvhtlyqsl hnislngigs
       61 msslnsmhsv isleeiqqtf neddstinnd tnetviegsc sdlrnrrgsr avsncgtafs
      121 lyfrdeirti dfifvwdefd sgaqtyrnte hrrvfeknle keglhleheq tepnglhfik
      181 vhaprevlrr yaeilklrla mkelpgmvip enrsytiine vnslfkrimr kyyvdqtifp
      241 nlkhnftavy srdkeylfdi dspnfftpat rarivqfild rtrftetnhn dfafgmerli
      301 serayvaayp lhdgdlqapd smryllykew aslrkclhyq pldyikeyfg ikiglyfawl
      361 gfythmlipa sivgllcfiy scatlysnep sedvcngngt ismcppcdyf cgywdlkqtc
      421 lharitylfd npstvffsvf mslwatlfle lwkrysveit hrwdltglda qeehprpqyl
      481 arlshikkks lnvitnteep kvpfwkmkvp atilsfsvvl lliavamaav lgvvlyrmsv
      541 ltalsvyghp mvtsyailft sataasinlc ciivfnwlyv wlaeylteie lprtqtefdd
      601 sltlkiylle fvnyyasify iaffkgkfvg ypgdynrffk tyrqeecgpg gclmelciql
      661 siimigkqam ntilemlfpl fykwlntlkv hvgmktkdgk kkittrkylq wikdyklvew
      721 gprslfpeyl emvlqygfvt ifvaafplap ffallnnvfe mrldakkllt myrrpvgqrv
      781 rdigiwyril dsisklsvit nafiiaftsn fiprlvyrit vsknhslegf lehslskfst
      841 sdfangtrpf aesgdehvei crypdyresp dspnkykyti tfwhiltarl afvvvfenvv
      901 alvmillrwc ipdinpklrd rirreayitn eiiiqqeair asersadere anrhfttine
      961 nanrwnrvmr nnlsssdfdl eihgspvssp pthsrvgpaa l
//
LOCUS       XP_034186647             941 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186647
VERSION     XP_034186647.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330756.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..941
                     /product="anoctamin 1 isoform X2"
                     /calculated_mol_wt=109452
     Region          62..282
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          285..865
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..941
                     /gene="subdued"
                     /coded_by="XM_034330756.2:473..3298"
                     /db_xref="GeneID:117607258"
ORIGIN      
        1 msslnsmhsv isleeiqqtf neddstinnd tnetviegsc sdlrnrrgsr avsncgtafs
       61 lyfrdeirti dfifvwdefd sgaqtyrnte hrrvfeknle keglhleheq tepnglhfik
      121 vhaprevlrr yaeilklrla mkelpgmvip enrsytiine vnslfkrimr kyyvdqtifp
      181 nlkhnftavy srdkeylfdi dspnfftpat rarivqfild rtrftetnhn dfafgmerli
      241 serayvaayp lhdgdlqapd smryllykew aslrkclhyq pldyikeyfg ikiglyfawl
      301 gfythmlipa sivgllcfiy scatlysnep sedvcngngt ismcppcdyf cgywdlkqtc
      361 lharitylfd npstvffsvf mslwatlfle lwkrysveit hrwdltglda qeehprpqyl
      421 arlshikkks lnvitnteep kvpfwkmkvp atilsfsvvl lliavamaav lgvvlyrmsv
      481 ltalsvyghp mvtsyailft sataasinlc ciivfnwlyv wlaeylteie lprtqtefdd
      541 sltlkiylle fvnyyasify iaffkgkfvg ypgdynrffk tyrqeecgpg gclmelciql
      601 siimigkqam ntilemlfpl fykwlntlkv hvgmktkdgk kkittrkylq wikdyklvew
      661 gprslfpeyl emvlqygfvt ifvaafplap ffallnnvfe mrldakkllt myrrpvgqrv
      721 rdigiwyril dsisklsvit nafiiaftsn fiprlvyrit vsknhslegf lehslskfst
      781 sdfangtrpf aesgdehvei crypdyresp dspnkykyti tfwhiltarl afvvvfenvv
      841 alvmillrwc ipdinpklrd rirreayitn eiiiqqeair asersadere anrhfttine
      901 nanrwnrvmr nnlsssdfdl eihgspvssp pthsrvgpaa l
//
LOCUS       XP_034186649             941 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186649
VERSION     XP_034186649.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330758.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..941
                     /product="anoctamin 1 isoform X2"
                     /calculated_mol_wt=109452
     Region          62..282
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          285..865
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..941
                     /gene="subdued"
                     /coded_by="XM_034330758.2:359..3184"
                     /db_xref="GeneID:117607258"
ORIGIN      
        1 msslnsmhsv isleeiqqtf neddstinnd tnetviegsc sdlrnrrgsr avsncgtafs
       61 lyfrdeirti dfifvwdefd sgaqtyrnte hrrvfeknle keglhleheq tepnglhfik
      121 vhaprevlrr yaeilklrla mkelpgmvip enrsytiine vnslfkrimr kyyvdqtifp
      181 nlkhnftavy srdkeylfdi dspnfftpat rarivqfild rtrftetnhn dfafgmerli
      241 serayvaayp lhdgdlqapd smryllykew aslrkclhyq pldyikeyfg ikiglyfawl
      301 gfythmlipa sivgllcfiy scatlysnep sedvcngngt ismcppcdyf cgywdlkqtc
      361 lharitylfd npstvffsvf mslwatlfle lwkrysveit hrwdltglda qeehprpqyl
      421 arlshikkks lnvitnteep kvpfwkmkvp atilsfsvvl lliavamaav lgvvlyrmsv
      481 ltalsvyghp mvtsyailft sataasinlc ciivfnwlyv wlaeylteie lprtqtefdd
      541 sltlkiylle fvnyyasify iaffkgkfvg ypgdynrffk tyrqeecgpg gclmelciql
      601 siimigkqam ntilemlfpl fykwlntlkv hvgmktkdgk kkittrkylq wikdyklvew
      661 gprslfpeyl emvlqygfvt ifvaafplap ffallnnvfe mrldakkllt myrrpvgqrv
      721 rdigiwyril dsisklsvit nafiiaftsn fiprlvyrit vsknhslegf lehslskfst
      781 sdfangtrpf aesgdehvei crypdyresp dspnkykyti tfwhiltarl afvvvfenvv
      841 alvmillrwc ipdinpklrd rirreayitn eiiiqqeair asersadere anrhfttine
      901 nanrwnrvmr nnlsssdfdl eihgspvssp pthsrvgpaa l
//
LOCUS       XP_034186696             428 aa            linear   INV 03-SEP-2025
DEFINITION  yellow-y [Osmia lignaria lignaria].
ACCESSION   XP_034186696
VERSION     XP_034186696.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330805.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..428
                     /product="yellow-y"
                     /calculated_mol_wt=48195
     Region          127..412
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..428
                     /gene="Y-y"
                     /coded_by="XM_034330805.2:156..1442"
                     /db_xref="GeneID:117607274"
ORIGIN      
        1 mycelllvsl lylasegvng lqrwasqfgq apllerfswr aldfaypdeg srrmammkge
       61 fipenalpvg ieiwrnklfv svprwrngip atlnyisldt nrggsprltp ypnwaqnkag
      121 acgsaittay rmhvdvcdrl wvldtgtigi gnttiqacpy tlnvfdlttd kimrqyrlra
      181 edinmntfia niaidlgkgg cndayaymsd elgyglivys weqnkswrit hsyfmpdpla
      241 gdynigglnf qwgeegifgm slspiaadgf rtlffhplss rrefavstri lrdedlsqds
      301 yhefqvlper galghctssv mdenglqffn lidqnavgcw nsllpyapen qalvakhdda
      361 mifpadvkvs rgvlwiisdr mpvfllsnln ytdinfrilt vpvkdaiagt vcentpwgyv
      421 snslwweq
//
LOCUS       XP_076548105             428 aa            linear   INV 03-SEP-2025
DEFINITION  yellow-y [Osmia lignaria lignaria].
ACCESSION   XP_076548105
VERSION     XP_076548105.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691990.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..428
                     /product="yellow-y"
                     /calculated_mol_wt=48195
     Region          127..412
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..428
                     /gene="Y-y"
                     /coded_by="XM_076691990.1:98..1384"
                     /db_xref="GeneID:117607274"
ORIGIN      
        1 mycelllvsl lylasegvng lqrwasqfgq apllerfswr aldfaypdeg srrmammkge
       61 fipenalpvg ieiwrnklfv svprwrngip atlnyisldt nrggsprltp ypnwaqnkag
      121 acgsaittay rmhvdvcdrl wvldtgtigi gnttiqacpy tlnvfdlttd kimrqyrlra
      181 edinmntfia niaidlgkgg cndayaymsd elgyglivys weqnkswrit hsyfmpdpla
      241 gdynigglnf qwgeegifgm slspiaadgf rtlffhplss rrefavstri lrdedlsqds
      301 yhefqvlper galghctssv mdenglqffn lidqnavgcw nsllpyapen qalvakhdda
      361 mifpadvkvs rgvlwiisdr mpvfllsnln ytdinfrilt vpvkdaiagt vcentpwgyv
      421 snslwweq
//
LOCUS       XP_034187200             340 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607522 [Osmia lignaria lignaria].
ACCESSION   XP_034187200
VERSION     XP_034187200.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331309.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="uncharacterized protein LOC117607522"
                     /calculated_mol_wt=37339
     Region          91..142
                     /region_name="bHLH_TS_dAS-C_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Drosophila melanogaster achaete-scute complex (AS-C)
                     proteins and similar proteins; cd19744"
                     /db_xref="CDD:381587"
     Site            order(95..96,99..100,102..103,125,128,132,134..135,139,
                     141..142)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381587"
     CDS             1..340
                     /gene="LOC117607522"
                     /coded_by="XM_034331309.2:1708..2730"
                     /db_xref="GeneID:117607522"
ORIGIN      
        1 mttmgvtvfc nrvqinsndq eqlmllrtlq dnesniignq phlivaknnn nsnpspsivl
       61 ppydpsrlkk hgkngqpppv avarrnarer nrvkqvnngf atlrqhipsh iaagygdrgk
      121 klskvetlrm aveyirglqr llaeadgvey dsnagaaqci psptssvvss shngsgerli
      181 leddvlavee edgeqleeed dngkqkatsr lspnrtavts prdyyassvg deenleprtv
      241 pnpqnfsrls pnsaasppse yygqneenle pqilspysga gdseegaatv yaaspetfsg
      301 alyykqeite sgefmdvvsw weqeqgrlvh qqhaqrlthv
//
LOCUS       XP_034186699             108 aa            linear   INV 03-SEP-2025
DEFINITION  chymotrypsin inhibitor-like [Osmia lignaria lignaria].
ACCESSION   XP_034186699
VERSION     XP_034186699.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330808.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186699.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..108
                     /product="chymotrypsin inhibitor-like"
                     /calculated_mol_wt=12083
     Region          55..108
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(64,68,80,84)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..108
                     /gene="LOC117607276"
                     /coded_by="XM_034330808.2:18..344"
                     /db_xref="GeneID:117607276"
ORIGIN      
        1 mkernqykse tfgtlftdsk ehhqslptik msrlllslla ilaifsvtvs skqvcpenee
       61 wqscasrcel tcekpdprpc tlecvrgcrc vdglirnssg kcvrraec
//
LOCUS       XP_034183196             159 aa            linear   INV 03-SEP-2025
DEFINITION  troponin C type IIIb [Osmia lignaria lignaria].
ACCESSION   XP_034183196
VERSION     XP_034183196.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327305.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..159
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..159
                     /product="troponin C type IIIb"
                     /calculated_mol_wt=17621
     Region          10..158
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..159
                     /gene="TpnCIIIb"
                     /coded_by="XM_034327305.2:147..626"
                     /db_xref="GeneID:117605693"
ORIGIN      
        1 mdspsamddi qlskdqiaql kmgfeafdpd kkgsitsdtv gtilgmmgmk iptqqllevi
       61 aefdpfgsge lsfqefcgla srfmeedtdt eamqqelrea frlydkegng yittdvfrdi
      121 lhelddalsp eeldmiidev dadgsgtvdf eefmevmtg
//
LOCUS       XP_034183198             153 aa            linear   INV 03-SEP-2025
DEFINITION  troponin C type IIIa [Osmia lignaria lignaria].
ACCESSION   XP_034183198
VERSION     XP_034183198.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327307.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="troponin C type IIIa"
                     /calculated_mol_wt=17195
     Region          2..149
                     /region_name="PTZ00184"
                     /note="calmodulin; Provisional"
                     /db_xref="CDD:185504"
     CDS             1..153
                     /gene="TpnCIIIa"
                     /coded_by="XM_034327307.2:144..605"
                     /db_xref="GeneID:117605694"
ORIGIN      
        1 mddltkdqia llkkafdafd hdkkgsigtd mvgtiltmlg yelsektlke iiqevdedgs
       61 gqlefeefct laarflveed seamqqelre afrlydkegn gyittavfrd ilhelddklt
      121 pqeldmmiee idadgsgtld fdefmevmtg gdd
//
LOCUS       XP_034183057             716 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605633 [Osmia lignaria lignaria].
ACCESSION   XP_034183057
VERSION     XP_034183057.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327166.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183057.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..716
                     /product="uncharacterized protein LOC117605633"
                     /calculated_mol_wt=80787
     Region          151..174
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          175..198
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          199..222
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          224..251
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          250..311
                     /region_name="LRR_8"
                     /note="Leucine rich repeat; pfam13855"
                     /db_xref="CDD:404697"
     Region          252..275
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          276..300
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          301..320
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          336..>368
                     /region_name="LRRCT"
                     /note="Leucine rich repeat C-terminal domain; smart00082"
                     /db_xref="CDD:214507"
     CDS             1..716
                     /gene="LOC117605633"
                     /coded_by="XM_034327166.2:1..2151"
                     /db_xref="GeneID:117605633"
ORIGIN      
        1 msttklkits vfdqafkqll sfawrkllaa vltlgitvfv iwlrykllss nseatknilw
       61 kqnlphvpqt snntiqqrit sycqsrdcks iscssipksl dpgteasnfi etdfkidckn
      121 veiiklsfnd ckfpenklrr nwlstnvsid eltfqscsld eieddifsds iykstkkiim
      181 isnqlhslrr gmfhhlesln eltiqnntir faefnlladv anslnvlels gainnretlr
      241 nitggsvpms kllilslren sigtierelf igvpsvtsly ldnskvetva qdtlepmass
      301 iiqlflnsnn ivtlpeglfd siikfrqafh ltatdnpwhc ncslkwiqdh vgtsssivig
      361 spicktpein agksftkanf cyqsnttssv ppvtstestk stkhdrsstt tlttkptkei
      421 inltcnfasi flqsgirlrk lmsndflqir sqfphfyisr dldqgvlvnl pdldqpvtll
      481 wfnnddvens lscvknvkhs ylvqnidpqt tyticllddn entvssplnc lavttsptye
      541 yrtwltnank slvfcvsifs livlflmgav iallmvrrhp illrgskrlm fvkrrnvdai
      601 vlpkgidide ekprnnvsfd qkfhedeyit plppasipvp rrsrvsrisv qsdwhsyvsq
      661 iepndtpley wrfarlnsel ekpkseappl pphrknavps lsmavesngd gyypsv
//
LOCUS       XP_034190731             328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609013 [Osmia lignaria lignaria].
ACCESSION   XP_034190731
VERSION     XP_034190731.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334840.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="uncharacterized protein LOC117609013"
                     /calculated_mol_wt=38301
     Region          58..178
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(147,154,156,162,164..167,170..172)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..328
                     /gene="LOC117609013"
                     /coded_by="XM_034334840.2:330..1316"
                     /db_xref="GeneID:117609013"
ORIGIN      
        1 mrgclflilc aagmsavvvq synvsinhtv enrrrigrqn enirryacpi gffrlkrycy
       61 ylsggtapwr dayfhckdrn atlaildrng kdrmlrkylm sdqftelerw iggifnwqqm
      121 awewgvtgek vvfrnfgrpq teqskqqyaw hcitidpalk ykwsprscve rkhyicevea
      181 griarrrkkt tdpfapqnqr lkprkkgkky skeprkptkq nkqrswrknf veqnannywv
      241 hgvklgsmpp snlrmghpkn rlrhhsnrtq anypkvsqvv pqgpeysafl angansrypq
      301 sltdlgktfs qysgkmnela seevlikp
//
LOCUS       XP_034182980             956 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605583 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182980
VERSION     XP_034182980.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327089.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..956
                     /product="uncharacterized protein LOC117605583 isoform X1"
                     /calculated_mol_wt=104157
     Region          153..268
                     /region_name="DUF4758"
                     /note="Putative sperm flagellar membrane protein;
                     pfam15950"
                     /db_xref="CDD:464951"
     Region          <209..474
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..956
                     /gene="LOC117605583"
                     /coded_by="XM_034327089.2:627..3497"
                     /db_xref="GeneID:117605583"
ORIGIN      
        1 mtskyfliai tiiyvtecsa qvykshsvet avvikpavvn dnfitqtvyg fldftttign
       61 tvmvfspqse ptggekgkks sgpiiqtkps etqekaipni qpskthktns eeetkkqakg
      121 snvvnsvieq nvinssedng irspksqepn thtqvvtksp vissqvqvks kdetpgvknn
      181 laepeydfls kqpaevidet yklinlrpss kahpkprptg rrekenptgl vtklggtivk
      241 eglttvhets vigtyingky aqvlqsssri lsgtppipeg kirpsstqri lktigpqqgk
      301 lkpqleptpt nqqeesslpl evlfsapgst vrstrknnsp nlprpkfrnk vnedydsgev
      361 qqpkqnknrs sttarpsfkn rsppttttep pvtrrrsgfr psnqpnlpsk qsnkskneqq
      421 pvspnpvpkl klprtqgrws ykttpkprii irkqidvedd prstpetstt egplnvddnk
      481 stttsvstst tsavttgqav naqrkievaa deeldpsese dvasvsvqdq phaeqilpve
      541 tinveistaa dlsnayfeia tikspytfqv gtlrntryvt vtstakksfs tidpsttisp
      601 tepltenlla ntaaayettl pldsavatlp aislesgqat ppletltetf sttqtllkth
      661 llpviyngnt tkltlvqtyn iarvvtatkt lppmdiyqfi pskalnefns kldeagselh
      721 leldvgdedr ddddipkrvv apsndqgdsd lepfksisvs kvksdsavss saepqltadq
      781 lallkyfgqq qpqvittsrp vvvletvyes hvipvvnngn tlystlsrpv gtvprtsyey
      841 gtstiapvlq pqlppqlppq lppqlppqlp lfpqqpqftv tssplvtqtl atvsdsrilk
      901 ltfgaktayt tlyssrvvpt eittyvtntv pvqptvpafp gyypppvayp pfpfvg
//
LOCUS       XP_076548106             901 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605583 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548106
VERSION     XP_076548106.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691991.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..901
                     /product="uncharacterized protein LOC117605583 isoform X2"
                     /calculated_mol_wt=98031
     Region          108..213
                     /region_name="DUF4758"
                     /note="Putative sperm flagellar membrane protein;
                     pfam15950"
                     /db_xref="CDD:464951"
     Region          <154..419
                     /region_name="PTZ00449"
                     /note="104 kDa microneme/rhoptry antigen; Provisional"
                     /db_xref="CDD:185628"
     CDS             1..901
                     /gene="LOC117605583"
                     /coded_by="XM_076691991.1:629..3334"
                     /db_xref="GeneID:117605583"
ORIGIN      
        1 mtskyfliai tiiyvtecsa qvykshsvet avvikpavvn dnfitqtvyg fldftttign
       61 tvmvfspqse ptggekgkks sgpiiqtkps etqekaipni qpskthktns eeetkkqakg
      121 snvvnsvieq nvinssedng irspksqlin lrpsskahpk prptgrreke nptglvtklg
      181 gtivkegltt vhetsvigty ingkyaqvlq sssrilsgtp pipegkirps stqrilktig
      241 pqqgklkpql eptptnqqee sslplevlfs apgstvrstr knnspnlprp kfrnkvnedy
      301 dsgevqqpkq nknrssttar psfknrsppt ttteppvtrr rsgfrpsnqp nlpskqsnks
      361 kneqqpvspn pvpklklprt qgrwsykttp kpriiirkqi dveddprstp etsttegpln
      421 vddnksttts vststtsavt tgqavnaqrk ievaadeeld psesedvasv svqdqphaeq
      481 ilpvetinve istaadlsna yfeiatiksp ytfqvgtlrn tryvtvtsta kksfstidps
      541 ttispteplt enllantaaa yettlpldsa vatlpaisle sgqatpplet ltetfsttqt
      601 llkthllpvi yngnttkltl vqtyniarvv tatktlppmd iyqfipskal nefnskldea
      661 gselhleldv gdedrddddi pkrvvapsnd qgdsdlepfk sisvskvksd savsssaepq
      721 ltadqlallk yfgqqqpqvi ttsrpvvvle tvyeshvipv vnngntlyst lsrpvgtvpr
      781 tsyeygtsti apvlqpqlpp qlppqlppql ppqlplfpqq pqftvtsspl vtqtlatvsd
      841 srilkltfga ktayttlyss rvvpteitty vtntvpvqpt vpafpgyypp pvayppfpfv
      901 g
//
LOCUS       XP_034191366             497 aa            linear   INV 03-SEP-2025
DEFINITION  putative malate dehydrogenase 1B [Osmia lignaria lignaria].
ACCESSION   XP_034191366
VERSION     XP_034191366.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335475.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..497
                     /product="putative malate dehydrogenase 1B"
                     /calculated_mol_wt=57020
     Region          25..442
                     /region_name="Rossmann-fold NAD(P)(+)-binding proteins"
                     /note="A large family of proteins that share a
                     Rossmann-fold NAD(P)H/NAD(P)(+) binding (NADB) domain. The
                     NADB domain is found in numerous dehydrogenases of
                     metabolic pathways such as glycolysis, and many other
                     redox enzymes. NAD binding involves numerous...; cl21454"
                     /db_xref="CDD:473865"
     Site            order(144..146,172..173,216..217,258,260..261,285,290,317,
                     394)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:133419"
     CDS             1..497
                     /gene="LOC117609309"
                     /coded_by="XM_034335475.2:1..1494"
                     /db_xref="GeneID:117609309"
ORIGIN      
        1 msasvcttvi agvledhnfn hicfvaesls niipnfyyki iyksskewks wlkkmctlyg
       61 wshtkspliw qeigithsni nyvgssyqfw eflqqyydik sqltredlea lqadllfahd
      121 ikeeiskspd vqerrritiv gagrsvcael vcqllmtkel wlthgiligl ydepgcffki
      181 kkiykdaggv gaglnsvmil nnvpeglkdc hiliyldsll reeyegtddl lqrnykdiek
      241 lsmqineyap symkvlfcsm gitcfyanim helvtkvpst nivavsshyg leliyplvns
      301 vgftlqnfgc ppvwgylgin qfvdvdhmiq kvhtypatrt qisqknvllp iqtqehselr
      361 wffymshnkk pyvdnlkrka ltqylvgrse dfpkckaicd llklwyskke niedeiislg
      421 iasdgsfgip kglvfsqpvy lkvladdsrv wipftdfpmp nmpfsifqnf idtatiikeq
      481 iiklkensef kekfsat
//
LOCUS       XP_034191092             253 aa            linear   INV 03-SEP-2025
DEFINITION  arylalkylamine N-acetyltransferase-like 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191092
VERSION     XP_034191092.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335201.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..253
                     /product="arylalkylamine N-acetyltransferase-like 2"
                     /calculated_mol_wt=28470
     Region          <168..218
                     /region_name="ArgA"
                     /note="N-acetylglutamate synthase or related
                     acetyltransferase, GNAT family [Amino acid transport and
                     metabolism]; COG1246"
                     /db_xref="CDD:440859"
     CDS             1..253
                     /gene="LOC117609189"
                     /coded_by="XM_034335201.2:1..762"
                     /db_xref="GeneID:117609189"
ORIGIN      
        1 msvneetnpp vsfpikppgp pkvwrvietv ikgkenapik fsiqeipedr ydevadymct
       61 yfipdepmck csngkndpef aesfrelwkk vlkhglsiaa ftenpnggkp iiaavnvlvf
      121 chkddkedya dsmpikprri ldvvldiskk aqvyekygvd rymgafglsv hpsyrgsamg
      181 ghllraredv greynievts tgftspisqk laercgfetl ieqnyeelvd ekgnllfpgi
      241 eaktlkimak rly
//
LOCUS       XP_034186466             272 aa            linear   INV 03-SEP-2025
DEFINITION  ras-related C3 botulinum toxin substrate 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186466
VERSION     XP_034186466.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330575.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="ras-related C3 botulinum toxin substrate 1"
                     /calculated_mol_wt=30071
     Region          47..>81
                     /region_name="PLN00023"
                     /note="GTP-binding protein; Provisional"
                     /db_xref="CDD:177661"
     Region          65..237
                     /region_name="Wrch_1"
                     /note="Wnt-1 responsive Cdc42 homolog (Wrch-1) is a Rho
                     family GTPase similar to Cdc42; cd04130"
                     /db_xref="CDD:133330"
     Site            order(66,96..97,99..100,102,104,113,117..118,120..122,128,
                     131)
                     /site_type="other"
                     /note="putative GEF (guanine nucleotide exchange factor)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:133330"
     Site            71..78
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133330"
     Site            order(74..79,118..119,121,177,179,220..221)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133330"
     Site            95..101
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133330"
     Site            96
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133330"
     Site            order(97,120,128,130)
                     /site_type="active"
                     /note="putative GDI (guanine nucleotide dissociation
                     inhibitor) interaction [active]"
                     /db_xref="CDD:133330"
     Site            order(97..98,122,128)
                     /site_type="other"
                     /note="putative GAP (GTPase-activating protein)
                     interaction site [polypeptide binding]"
                     /db_xref="CDD:133330"
     Site            order(98..99,128,131)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:133330"
     Site            118..121
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133330"
     Site            order(121..122,128..131,136..138)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133330"
     Site            176..179
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133330"
     Site            219..221
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133330"
     CDS             1..272
                     /gene="LOC117607193"
                     /coded_by="XM_034330575.2:1..819"
                     /db_xref="GeneID:117607193"
ORIGIN      
        1 mkayrcdkgk kkimppssht nwtkpllkng qtmqmqtkrt tcsqqnqnsn gggtehngsp
       61 ldrrikvvlv gdgavgktsl vvsystngfp geyvptafdn ynvvvnvdgq pvniqlcdta
      121 gqddfdplrs lcypetdvfl vcfsvvcpss yhsvtshwvn evkkycpnap iimvgtksdl
      181 rsdvhlmlql arygqtpitt tqghqlaqrv gavsyvetsa ltqhdlkeaf dqaivsalnt
      241 rrggasllyr rrkplslwrr wlccwerpss ga
//
LOCUS       XP_034182997            1045 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605590 [Osmia lignaria lignaria].
ACCESSION   XP_034182997
VERSION     XP_034182997.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327106.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182997.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1045
                     /product="uncharacterized protein LOC117605590"
                     /calculated_mol_wt=112107
     CDS             1..1045
                     /gene="LOC117605590"
                     /coded_by="XM_034327106.2:146..3283"
                     /db_xref="GeneID:117605590"
ORIGIN      
        1 mrilwiativ avlstynasa ssekskraie tnggyiyepp nniftlptrt pprptqppvt
       61 srpvtpgyps hdnqytlptq rptytpprte gpgtrptgyt ypippqptrp pgppprpntp
      121 ppyrppsppq ptsppyrpst gydypapvqp ptlapyrppp spprppsppq ppappapspt
      181 hppqtprpps pptyrppapp tygppsppty gppspptyrp papptygpps pptygppspp
      241 tyrppappty gppspptyrp qpqpprppqp tagydypvps ktfpprppqp ptpppyrppp
      301 qpqppktpap pppfptqppq tprppqprpp tpppylppsp pqpprppspp pyvppspphp
      361 pqtprppqpr ppspppyvpp sppqtprpvp pyrppsppqp pqtprppqpr ppspppyvpp
      421 sppqtprpps pppyvppspp qppqtprpys ppnrppttgy typappqptr ppppqprppt
      481 ppqppqtprp pqlrppspps ylppsppqtp rppptygpps ppqppqtprp pqprppsppp
      541 yvppspsqtp rppptygpps ppqppyrpps ppqtprppsp prphtppeyl ppappqsprt
      601 paprppyvpp tpgphqpptf ppygppqppq pprppqtpfp pgpptrppyv qppqppqtpr
      661 ppsppqtqpp fgppsrpylp ptpptrppyv pppqpprppq tpfppgppsr ppyvpppqpp
      721 qpprppsppr ppqtplppgp psrppyvppp qppqtprpfp pqppktpapp gppsrpyvpp
      781 qptppirppv tpspprpptq pntylppdrp gptppprptp gyptpprptp gyptpppfst
      841 prptpghptp ppfssprptp ghptpppfst prptpgyptp ppfssprptp ghptpppyst
      901 prptpgypsp htqpntylpp dsskatfptr ptpgystfps pntqgptvti phgstqtpyq
      961 ptggvpatrp ppylppstps sprttvtppp pptpiytlvp tstiytpppt gnpappptlp
     1021 pttnrptgyf ypvpenpfel ppflr
//
LOCUS       XP_076548107             116 aa            linear   INV 03-SEP-2025
DEFINITION  cytochrome c oxidase assembly factor COX20 lethal (3) 87Df [Osmia
            lignaria lignaria].
ACCESSION   XP_076548107
VERSION     XP_076548107.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691992.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="cytochrome c oxidase assembly factor COX20
                     lethal (3) 87Df"
                     /calculated_mol_wt=12791
     Region          23..102
                     /region_name="Cox20"
                     /note="Cytochrome c oxidase assembly protein COX20;
                     pfam12597"
                     /db_xref="CDD:403707"
     CDS             1..116
                     /gene="l(3)87Df"
                     /coded_by="XM_076691992.1:1..351"
                     /db_xref="GeneID:117607350"
ORIGIN      
        1 mehsndpqek nfqptftlfg epiiktrcqr nalisgisgg ilsglvaflf tsqprrsvyi
       61 gfggylaitf cmtcyctyde yslrkqarli ktaiyeinas kkgsessnss lkveda
//
LOCUS       XP_034191156             387 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609219 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191156
VERSION     XP_034191156.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335265.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191156.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..387
                     /product="uncharacterized protein LOC117609219 isoform X1"
                     /calculated_mol_wt=44757
     Region          <84..>324
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..387
                     /gene="LOC117609219"
                     /coded_by="XM_034335265.2:103..1266"
                     /db_xref="GeneID:117609219"
ORIGIN      
        1 mscvhyqssd psrdrsyydg qcnrfvsssy nprnqtadty rsgycagwrm hefqgnnvet
       61 ptpgkgeptf sdprihtpra tykqettihg dqlaiekmqt kikflensni vmqtrnqkli
      121 tenkalmsrl eeeqdevrgl ekkitslqee ldcnklklee mkkeaeaskk nacmlkanat
      181 sttnipridr gvqvwavcmg crkklescek qpptvtitks elevlekdmq tlrdtiiare
      241 eawdkamere enyrqqltrl ttetitarhl setrhdelkt mtnalsekes elktlekdnf
      301 nlrkllakhy nvyqrgqegi einekdqryi eevarmtsvk skqkqksksa csertphtia
      361 hqtaisprer svktmkdqts slretkr
//
LOCUS       XP_034191157             385 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609219 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191157
VERSION     XP_034191157.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335266.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191157.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..385
                     /product="uncharacterized protein LOC117609219 isoform X2"
                     /calculated_mol_wt=44499
     Region          <84..>322
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..385
                     /gene="LOC117609219"
                     /coded_by="XM_034335266.2:103..1260"
                     /db_xref="GeneID:117609219"
ORIGIN      
        1 mscvhyqssd psrdrsyydg qcnrfvsssy nprnqtadty rsgycagwrm hefqgnnvet
       61 ptpgkgeptf sdprihtpra tykqettihg dqlaiekmqt kikflensni vmqtrnqkli
      121 tenkalmsrl eeeqdevrgl ekkitslqee ldcnklklee mkkeaeaskk nacmlkanat
      181 sttnipridr gvqvwavcmg crkklescek qpptvtitks elevlekdmq tlrdtiiare
      241 eawdkamere enyrqqltrl ttetitarhl setrhdelkt mtnalsesel ktlekdnfnl
      301 rkllakhynv yqrgqegiei nekdqryiee varmtsvksk qkqksksacs ertphtiahq
      361 taisprersv ktmkdqtssl retkr
//
LOCUS       XP_034186829             481 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607342 [Osmia lignaria lignaria].
ACCESSION   XP_034186829
VERSION     XP_034186829.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330938.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186829.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="uncharacterized protein LOC117607342"
                     /calculated_mol_wt=52093
     CDS             1..481
                     /gene="LOC117607342"
                     /coded_by="XM_034330938.2:1..1446"
                     /db_xref="GeneID:117607342"
ORIGIN      
        1 mrrqsyntnl stgsggepqi iveestlgee eaerrrnesp prcldpdsps lnpyllspwr
       61 etrkhslptp qctsgitasq vrrlsergge gsgpspreaa flatlsqapa pqsggrrhsv
      121 vtisrvpptl fgrnrresia afplggatri lpgrrdsksg isgppsnsgs thnlqldimd
      181 diaeikarkv rlkmyktste qvceiqpleg nsstqrytqy qkrrfselpp pamsptsslr
      241 rraselprav stsvagaagi vcsntdlisi lsslassate inrcgeessr edsksswsgk
      301 tteqkrnrlk sfrsnsfdvs ilhgakskla gsskattasv mapsnwftkr hqpmskkqkp
      361 edlitaslnf kfdkskvvka vketlgrtsp tsetrhkvvw dgssgtkvda qvlgsaieki
      421 ltqrggdtet psastskasi spnkpkaskv tswfsgnnkd qeqnescepg icsslkdlfv
      481 k
//
LOCUS       XP_034186940             894 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607399 [Osmia lignaria lignaria].
ACCESSION   XP_034186940
VERSION     XP_034186940.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331049.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186940.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..894
                     /product="uncharacterized protein LOC117607399"
                     /calculated_mol_wt=92006
     Region          <52..248
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          <151..>385
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..894
                     /gene="LOC117607399"
                     /coded_by="XM_034331049.2:1..2685"
                     /db_xref="GeneID:117607399"
ORIGIN      
        1 mhvtpstmki fvvcfvtwal inqeavrats ltasrsddeh hltswsssst saeelenieh
       61 rnirsahles sgssissssr kveskqerss svdtkgqdeg dgkhvslkkv eisqhdvrqk
      121 ddkkggkeve kkvivketkn tatkssssst eiktlkeeki kaeararkes earskaekea
      181 aealrklkel aaalkqaeak akseanalae ikakeeaean alaaaraqik akseeaaqai
      241 qkakleaqar tiiqsnilpi vkraiakeda vingelwkka qlaaktaaas saasqkavka
      301 ikaalaarae asakvkaall aiiagseasc nakaaavass ialrqaeiss kaaanaqvha
      361 ldmtrdaeri asgeegavey aielatkskn ianqsklksn saaeasgsaa aaseeleklq
      421 rnartlakda nsaqkaalde anvlgaetsl vardvmaasa rsdveaaiie aeaaarasaa
      481 gaigdgvivg lgetadsnla qakelkkavn gvaknlnnii eccsqsdtwg ivwtigrdgi
      541 kslvgrcras ireassnmki pallatclfv wgsvsasdgh hggkgiplle makggasitl
      601 saavsakagl ragqvaeasq keaaiqsgaa ageaakargl adqtaklseq samlqsqaaa
      661 kskaaeeaat aeqnaqlear aiaaqataaa keaasgarsa aeaastaale aaiaakavqv
      721 qaalslkske kameaankaa aasnaakaaa qanraavmil akataakaea raamaalasa
      781 kvaardgans aaeaearnev aqliaaidqk sreidaglsm kaqaiakssa rnneaaviga
      841 nidsakrivk vpqkviipkh esswqsskee kievdvkqvd vknvhsessg swsh
//
LOCUS       XP_076548108             680 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607422 [Osmia lignaria lignaria].
ACCESSION   XP_076548108
VERSION     XP_076548108.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691993.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..680
                     /product="uncharacterized protein LOC117607422"
                     /calculated_mol_wt=75314
     Region          91..215
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(189,193,199,201..204,207..209)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Region          321..445
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(419,423,425,429,431..434,437..439)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     Region          550..674
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(648,652,654,658,660..663,666..668)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..680
                     /gene="LOC117607422"
                     /coded_by="XM_076691993.1:162..2204"
                     /db_xref="GeneID:117607422"
ORIGIN      
        1 msaltilvnc iilcvafrgt fsmpkkiavl tinttdevdd tqsvqrptkl hwdqyqtrnm
       61 mvhgtvcvcn lrlrgkpprd dytytagigs hkfhsrakew ntarkicnee gghlavinsy
      121 aeqraltelf krggkvvgst fkdtaflglh dlyfegdwvt ilgdplavtg ftvwssawgg
      181 qpdnaggvqh cagmmmdgtm ddincnipya ffceipelap ltqskemsrl silvnciilc
      241 vafrhtvsvp kriavvsfnk teniliskts dsslrhples ywdelrnsdv nvhgtvcmfd
      301 lgirgvrpre dymhtpgigs hklhtrgqtw ntariicnee gghlaivnsd eeakamvrlf
      361 hiygqfkdaa rndeafigth dmykegdwat ilgeplaetg ftkwsnlfgg qpdngygdqn
      421 cggflnegtl ddihcyarfa ficeipeiip lrirkagvva ksintlsvac lseltrvngi
      481 kgdmsplsil vnciilsvaf qgtfsmpgnv avptpsiqni taqlvyvcdi gmrgayrrdd
      541 ythtpgigsh klhtkaekwn qarkicndeg ghlaiinsna erralvelfd rsgsvvgadh
      601 tfeallgihd lyaegdwvtv fgepiaktgf vewsdrwngq pdnwghnqhc gamlkdgtmd
      661 dvrcdkafaf fceipeitpl
//
LOCUS       XP_034186979             727 aa            linear   INV 03-SEP-2025
DEFINITION  sprouty signaling antagonist, partial [Osmia lignaria lignaria].
ACCESSION   XP_034186979
VERSION     XP_034186979.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331088.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186979.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: incomplete on the amino end.
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         <1..727
                     /product="sprouty signaling antagonist"
     Region          571..681
                     /region_name="Sprouty"
                     /note="Sprouty protein (Spry); pfam05210"
                     /db_xref="CDD:461589"
     CDS             1..727
                     /gene="sty"
                     /coded_by="XM_034331088.2:<1..2184"
                     /db_xref="GeneID:117607418"
ORIGIN      
        1 ngsaesvdtl dyepppvlpp rrppwpsnsp sstasryigq rtatitttss ltptaatttt
       61 tsttpltttl vpapsvsqgs ttsssarvsp qppppltppq ypapprppsr nggdearvar
      121 argsgrgalp siilangtpl prvpppragw avrinettht tptqsthlhq qqqqqqqqhl
      181 rnnhisspgt praieqwase raassalsvr gctspsspev prrsgtgsrr vvassnrvra
      241 gagagsagsg deegrggstg iaggvnfasd wqeeeeleak kkeeqrkrsa dsvarcqaeq
      301 lelrgrpmsq nnaisplsrp pssqqstnvh dattdavldg sntddgeays vqqgrvhdhs
      361 rpfppprlpt vhttattlat apphpsnqsp rtlrtaplsp lsntfrsrpr ntdasnetnt
      421 rgllstdrsp prspigevsl tvprpdgert ineyveapfk hcanhqerry dedkrgvadc
      481 pkidrrhhrq kgaadasvaa thrlhatraq afralnanat naaasagsvn tpttnpvtkq
      541 pvsftkepan slasrtydpa glsimcnfcg rcrcescrep pplpskwlcd nkcfcsadti
      601 ldyasclccv kglfyhcmdg ssasgidgeg vgscadepcs ctgnkrasrw aclsaltlvm
      661 pcllcywpfk gcvalcelcy arhaaqgcrc npntlgnagn rhhpitndis dsrdpekrll
      721 dpvtpel
//
LOCUS       XP_034186978             395 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607417 [Osmia lignaria lignaria].
ACCESSION   XP_034186978
VERSION     XP_034186978.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331087.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..395
                     /product="uncharacterized protein LOC117607417"
                     /calculated_mol_wt=45845
     Region          60..382
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..395
                     /gene="LOC117607417"
                     /coded_by="XM_034331087.2:1..1188"
                     /db_xref="GeneID:117607417"
ORIGIN      
        1 mdsaqykylr nckyylciig lwpyqtflqk flvriiyipi iatqvilqgg glvaasvasd
       61 tesflegfap fiislmclak yinfiynydq mkrlldimqe dwkiykkfdk eydiirqqya
      121 egnrivtnfg gllfgmitpf agmscilnaa dalglynlng erpltfrieh fvdvdkyyyi
      181 llvhsyigti ayaaivvavd sviiifvihe cglceilryk lencvetdsm didlhpdkqe
      241 dmwyqnaksc vklhkhiief adiienantm syffqlgfnm icvsftqfqa ivhlsapnka
      301 lqyvsltisl lcdllfvswp gqrlidyter ifehttnnkw yqssintrtl lrimllksit
      361 tpkltackly tlnmenfstv rfiiinkilf lyykh
//
LOCUS       XP_076548109            2844 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605672 [Osmia lignaria lignaria].
ACCESSION   XP_076548109
VERSION     XP_076548109.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691994.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2844
                     /product="uncharacterized protein LOC117605672"
                     /calculated_mol_wt=303702
     CDS             1..2844
                     /gene="LOC117605672"
                     /coded_by="XM_076691994.1:134..8668"
                     /db_xref="GeneID:117605672"
ORIGIN      
        1 mkgvtilllv llvhtfdahn khegrmtkle kkhtlkreaa vekirklyek ldekdseese
       61 rrkppnisnl pawkrkpdss dsdcdssstk dssncessss tdeirssksr lhkqsnskhe
      121 kydsrrqkkg dhaighkatl nllenfeeqf krllkklipr vkerihiiqm lkyhriescq
      181 slsplqffyk rlyeynkary mawrkkleir nyilshmnhl daeaimrkcn iqrnipyalr
      241 raqmkrcinv yasinkifki vtklprpsln padeilwlcn ktslhksstt veknskdtet
      301 takiptqada dnypspvtdt esknhtstte strhrgsika psstttsket entttsrift
      361 issavettte atntiqghit ttssstkpss lthrkrltts svpteqpspt iiiqnknrtp
      421 srerpktwsg apttnagtat iegqkktiis nskpslvttv ktivnrssed tptsniiqer
      481 ittlskerpi awssahtttk rpatvarkee viisgskppl vttvktivnr psehmptani
      541 iqerittlsk erpvawssah tttkrpvtva rkeeviisgs kpplvttvkt ivnrpsehmp
      601 tantiqerit tlskerpvaw nsahtttkkp atvarkeevi isgskpplvt avntivnrps
      661 ehtpraniiq erittlsker paawssahnt tkgpatvprk ekviingskp lslttattlr
      721 qtsirgsths sgptehtssa nviqeristl prespaawss ahttskrpaa vaseeeviis
      781 dnepsllpta ktightstda stnhpgpsqh tsaanmmqer isplsregpv awssahttsk
      841 rpaavaseee viisdnepsl gttaktight stggspnhpg pseptstgni iqesgssqar
      901 erpvawnsah tttkipatva reeeviisdn kpsllptakt ightstdgst nhpgpsqhts
      961 aanimqeris plsrerpvaw ssahttskrp aavaseeevi isdnepslgt taktightst
     1021 ggspnhlgps eptsagniiq esgssqarer pvswnsahtt tkipatvare eeviisdnkp
     1081 sllptaktig htstdastnh pgpsqhtsaa nmmqerispl sregpvawss ahttskrpaa
     1141 vaseeeviis dnepslgtta ktightstgg spnhpgpsep tstgniiqes gssqarerpv
     1201 awnsahtttk ipatvareee viisdnkpsl lptaktight stdgstnhpg psqhtsaani
     1261 mqerisplsr erpvawssah ttskrpaava seeeviisdn epslgttakt ightstggsp
     1321 nhlgpsepts agniiqesgs sqarerpvsw nsahtttkip atvareeevi isdnkpsllp
     1381 taktightst dastnhpgps qhtsaanmmq erisplsreg pvawssahtt skrpaavase
     1441 eeviisdnep slgttaktig htstggspnh pgpseptstg niiqesgssq arerpvawns
     1501 ahtttkipat vareeeviis dnkpsllpta ktightstdg stnhpgpsqh tsaanimqer
     1561 isplsrerpv awssahttsk rpaavaseee viisdneppl gttaktight stggspnhlg
     1621 pseptsagni iqesgssqar erpvswnsah tttkipatva reeeviisdn kpsllptakt
     1681 ightstdast nhpgpsqhts aanmmqeris plsregpvaw ssahttskrp aavaseeevi
     1741 isdnepslgt taktightst ggspnhpgps eptstgniiq esgssqarer pvawnsahtt
     1801 tkipatvare eeviisdnkp sllptaktig htstdgstnh pgpsqhtsaa nimqerispl
     1861 srerpvawss ahttsktpat vareeeviis dnkpsllrta ktightstdg stnhpgpneh
     1921 tsagniiqes vssqaterpv awnsahtttk rpaivareeg viisdnkqsl lptaktight
     1981 stdgstnhpg pnehtsagni iqesvssqar erpvawnsah tttirpatva reeeviisdn
     2041 kpsllptakt ightstggst nhpgpsehts aaniiqervs sqprdrpvaw ssahtttkrp
     2101 atvareeevi isdnkpsllp taktightst dgstndpgpr ehtsagniiq esvsaqates
     2161 pvawnsahtt tkrpaivare eeviisdnkp sllptaktig htstdgstnh pgpnehtsaa
     2221 niieervssl srersvtwss ahtttktpaa vareeeviis dnkppllpta ktightstda
     2281 stnypgpseh tsaaniiqer vsslprdrpv awssahttsk rpaavareee viisdnepsl
     2341 gttaktight stggspnhpg pseptsagni iqesgssqar erpvawssah tttkrpatva
     2401 reeeviisdn kpsllptakt ightstdgst nhpgtnehts agniiqesvs sqarerpvaw
     2461 nnahtttkrp aivareeevi isdnkpsllp taktightst dgstnhpgpn ehtsagniih
     2521 egvsslsrer pvtwssahtt tktpaavare eeviisnnep sllptaktig htstdgstnh
     2581 pgpsepmsaa niiqervssl srerpvtwnr antttktpaa vareeeviis dnkpslgttv
     2641 ktaghsstgg ltsgprpteh mlsgniiqgk vstlswerpt pwikadtise gtaktawqre
     2701 aiisgsksss vkivetight sterptsrsk pteqtspest iydrintiss stptiwssan
     2761 tttdgtvtaa khgetiisns kpslgtiiet ivhtatdrpt septeqtssg tiiydrinti
     2821 ssgtpitwsg adttivgtvt avgh
//
LOCUS       XP_034183288             250 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605738 [Osmia lignaria lignaria].
ACCESSION   XP_034183288
VERSION     XP_034183288.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327397.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 2% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..250
                     /product="uncharacterized protein LOC117605738"
                     /calculated_mol_wt=28418
     Region          19..>122
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          37..57
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(42,44,46,48..49,52..53,56,70,72,76..77,80..81,84,98,
                     100,102,104..105,108..109,112)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          65..85
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          93..113
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          121..137
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          149..169
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     Site            order(154,156,158,160..161,164..165,168,182,184..186,
                     188..189,193,196)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275370"
     Region          177..197
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275370"
     CDS             1..250
                     /gene="LOC117605738"
                     /coded_by="XM_034327397.2:1..753"
                     /db_xref="GeneID:117605738"
ORIGIN      
        1 msvrgvvqda pldlscrgse kklrlanssk fqrclpcqtc gksfdrpsll krhlrthtge
       61 kphgctvcgk mfstssslnt hvrihtgerp hecpicgkrf tassnlyyhr mthykekphk
      121 cnecarsfpt pgdlrahgys hsgnwpmrcl icnrgfckpg alhhhmqlht gnrpyccnic
      181 kkqfslisnl rshershdld msiasvtnys steskvptms tirfedtskn hlqfptiysw
      241 sswvpihypk
//
LOCUS       XP_076548110             783 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605648 [Osmia lignaria lignaria].
ACCESSION   XP_076548110
VERSION     XP_076548110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691995.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..783
                     /product="uncharacterized protein LOC117605648"
                     /calculated_mol_wt=90016
     CDS             1..783
                     /gene="LOC117605648"
                     /coded_by="XM_076691995.1:1..2352"
                     /db_xref="GeneID:117605648"
ORIGIN      
        1 mkqavsgnfs fntiafnfyq lstssiiglg qlfsqiyvsl tilwvsvnar fyedrfksls
       61 qhepwasdie tqdfasseys psyvhfpesg kqetfesses ydrpnkewfs assskrvple
      121 dkhfkkiisp fytitekdsq kykdivmksg ipycqeiktk kpskdgnkns mvcykcknpk
      181 tgatyeqcsy vskpsadssn qeevvddpsg frnrrsnsde gsysfrgsdd yrrhdnpyrf
      241 nekffsdase gvpaeyknkd ekcekvfkds mvcmvckntk snakyeqcsy vrqpnekrya
      301 ftksssfknp kekeeaedrd eeseskpvre rtsfdhkkes eklsdgykkt pdncrevekd
      361 sktctickdp etggnyekcs ytyqpddkvy kysssksfgh prkssssddd ssykddkpse
      421 kltretddsg ykddkhsekl tkeadeeykr dysipessyf kddqpeyiyh paasetskst
      481 sededplagy erskseseki aeriepsyck rvqkgslsck vckdpktgsn seqcsyeqqp
      541 sdknysysks ksfrtptete dksydgsekk eshspykdqs fgffgdekps erdardysts
      601 daeskveekk estsrstpkk tevdfsdafk rkaeiqkflq efqkedrsnc kklmrnkmtc
      661 yqcvddkgfr keecafitdg diaedkidet evepnrkvpr sipedvpaep evsaseniev
      721 nrrkpetass eeaekpkeae ayeyvaetkp vfdkvlgitl paymlatseh eeefdrivvs
      781 dvv
//
LOCUS       XP_076548111             338 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605735 [Osmia lignaria lignaria].
ACCESSION   XP_076548111
VERSION     XP_076548111.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691996.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 16% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..338
                     /product="uncharacterized protein LOC117605735"
                     /calculated_mol_wt=39395
     Region          73..329
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..338
                     /gene="LOC117605735"
                     /coded_by="XM_076691996.1:86..1102"
                     /db_xref="GeneID:117605735"
ORIGIN      
        1 mdrdkieqqy lkvtkifanl agtwpyqrpl skliiiiiss litipaiytq vliearilff
       61 fkqngntrkt nqffhlrfeg edlkllldgm yedwtvnksk eeivimktya eratyftsfy
      121 aitaifttvf finmplisri ldkliplnes rdplymyepy ygwdeekyfy vtvthmsifv
      181 ililfvftav dtifiyfvqh acgllevsgh rfkhaiedfs scekkimdpa qelsimeing
      241 dteccfhcvf lfvqlmhmff tvlqgqfvin aiddvydhiy ealwynvppk tqalyllalr
      301 rtltppeisa agvvtfnlqs fsaiiktsvs yfmvlknt
//
LOCUS       XP_034183284             216 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605734 [Osmia lignaria lignaria].
ACCESSION   XP_034183284
VERSION     XP_034183284.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327393.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183284.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 29% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..216
                     /product="uncharacterized protein LOC117605734"
                     /calculated_mol_wt=24621
     CDS             1..216
                     /gene="LOC117605734"
                     /coded_by="XM_034327393.2:53..703"
                     /db_xref="GeneID:117605734"
ORIGIN      
        1 mddigiqqry lkltkrclii sgiwpkqtks tknilrfivy ittvpcimsq iayvacffsl
       61 tavveqlsfl lamvlglfkq gayivneekf kgllngmfnd wavdrskeel aimtsymerg
      121 alfasiylan aysctglfmq mpwwpriadl vmplnesrpr ayifpayyfi ddtnyyywii
      181 thmsiatvlt tiiyiacdtc yiyavqhacg llavag
//
LOCUS       XP_034183283             323 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605733 [Osmia lignaria lignaria].
ACCESSION   XP_034183283
VERSION     XP_034183283.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327392.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183283.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 52% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..323
                     /product="uncharacterized protein LOC117605733"
                     /calculated_mol_wt=36546
     Region          <71..314
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..323
                     /gene="LOC117605733"
                     /coded_by="XM_034327392.2:1..972"
                     /db_xref="GeneID:117605733"
ORIGIN      
        1 mvagystqva rlvvfcsvnt fltdipflvv algtiiktsn ciinesknin niilnstnqi
       61 nflvvhmtvs ttlfstmpfv pplldiilpl nksrepmysy payygvdnek yhfvivahmt
      121 ailfftnvly cacdmnyvyi vqhacsllai tgqrfkcacy dqdtcnklkm rriyrnvcds
      181 irthqralty vnqvgsaydi slfvsmglll gsitlsllmv tssefgmmwf iynvflltql
      241 lhvfflvvqg qfvinsednl yktigeatwy natnetrtly vlalrrsltp plltaggfvk
      301 ldlktfteil rasvsyytvm ksk
//
LOCUS       XP_034183041              72 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605620 [Osmia lignaria lignaria].
ACCESSION   XP_034183041
VERSION     XP_034183041.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327150.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..72
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..72
                     /product="uncharacterized protein LOC117605620"
                     /calculated_mol_wt=7935
     CDS             1..72
                     /gene="LOC117605620"
                     /coded_by="XM_034327150.2:122..340"
                     /db_xref="GeneID:117605620"
ORIGIN      
        1 maclkiytvl llaviigivs akpgylggyg ygyypfgygy rypfygyghg fgyygyphyg
       61 yghgfghygg yh
//
LOCUS       XP_076548112             603 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605556 [Osmia lignaria lignaria].
ACCESSION   XP_076548112
VERSION     XP_076548112.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691997.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 8% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..603
                     /product="uncharacterized protein LOC117605556"
                     /calculated_mol_wt=67971
     CDS             1..603
                     /gene="LOC117605556"
                     /coded_by="XM_076691997.1:1..1812"
                     /db_xref="GeneID:117605556"
ORIGIN      
        1 msrtkrklpg trsqvahytg lryavqrear iflmetkerk vtthllivaf vllqitvlca
       61 tfwilqddvy aaknglnaev ltsfaknvas iistsllgey qeqstnqfps sllkkiqddp
      121 sllnaenien gsleeaskfs snrqrrqars skevrgafqd gyitpnplgy skyynpepyk
      181 pyeytanhdg risgkedvfn vqtskntnvd trgnsidadi naasvyysdr yrypaghrhn
      241 snpvhplvns nlhpyfgfqp vyhhsppqna ggpgqpseie engnenarrr pynlnyqanf
      301 hapnhrhvgf yphgrpiegn gqpsppvngq desnpsrlid dqnqgqstlg gydysphfhy
      361 rppyhggfyh gfpdfpyhph nvsdnpgyge qggnssggyp pygppgfpdy kynpypyysp
      421 fppyyhgprr pvepqnppen sgngepnmsp engqadgngt vqnrppygfp pygpvygdfh
      481 fpgyplvgyn gfpyhhfnhg fhnhlfpyfk vgpyphfplg pwynhfggyh rpyngrkpmm
      541 mpdkpmqddk stsenaegsp pnsdaemiae skaeitenke lpswlfteei kkpvsdqsie
      601 kis
//
LOCUS       XP_034190765             374 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor 67c-like [Osmia lignaria lignaria].
ACCESSION   XP_034190765
VERSION     XP_034190765.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334874.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190765.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 1% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..374
                     /product="odorant receptor 67c-like"
                     /calculated_mol_wt=42294
     Region          57..361
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..374
                     /gene="LOC117609038"
                     /coded_by="XM_034334874.2:86..1210"
                     /db_xref="GeneID:117609038"
ORIGIN      
        1 mtfpstlsps vryglhfigv wpgtplpglh klcwviamvl lqtyqyryii qhyhtdslle
       61 midnlsiamp fslvcikliv awtnhdilrd ilstmdkdcq kyaaidtnnl isktarisly
      121 fttivissyv fsaisyltgt lamnvgdnst rrllfqmdlp fetnespaye lvvsaqflhl
      181 atsaftfgvf savllmvvlh vgchidvmck vilddplknk eqinffinrh neiilfadri
      241 eklftyials qlmsdtlitc cvgylivmsv htenglplli rsvlfyvvic lealiycfsg
      301 eylnskskli gntiyetdwc dlhpresrll vflilrsqkg ftftcgkfst lslesftsim
      361 kisasyvsfl lais
//
LOCUS       XP_076548113             234 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609067 [Osmia lignaria lignaria].
ACCESSION   XP_076548113
VERSION     XP_076548113.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691998.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 17% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..234
                     /product="uncharacterized protein LOC117609067"
                     /calculated_mol_wt=26411
     Region          <104..>233
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..234
                     /gene="LOC117609067"
                     /coded_by="XM_076691998.1:231..935"
                     /db_xref="GeneID:117609067"
ORIGIN      
        1 mkvtstisps vkyglhfigi wpgtpfpmfh kiywiliiaf fqtyaykyii qhfqsaslte
       61 tidnilyfin fyqkyasidi nnlllktagf fycltttivt lyalsavfhv agtaaslssi
      121 egneslslll kmdlpfetnt spsyellvia qflhqttgaf sfgvfsalvl ivvlhagyqi
      181 dimcqtimdv slqdkeqlkl fiirhqdviv fvekigkyft fialsqlavw afls
//
LOCUS       XP_034190726             210 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609008 [Osmia lignaria lignaria].
ACCESSION   XP_034190726
VERSION     XP_034190726.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334835.2
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190726.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 9% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..210
                     /product="uncharacterized protein LOC117609008"
                     /calculated_mol_wt=25520
     CDS             1..210
                     /gene="LOC117609008"
                     /coded_by="XM_034334835.2:1..633"
                     /db_xref="GeneID:117609008"
ORIGIN      
        1 mihacpqycf llaffillhe tiqseeiykl irpykyyqrr psrwprfhag hkssrfyrfg
       61 ripsedyysr rstyyrkine mlvrrfryrs srndfdgrae nhpytiviql pkaetkyrdn
      121 nyrerqrnye rdtvptynne sdyideeeem eskvgninnk kvqikmikgk npklhiqisk
      181 sdnennleiv dkssissmnl prlfnyktii
//
LOCUS       XP_076548114             560 aa            linear   INV 03-SEP-2025
DEFINITION  LOW QUALITY PROTEIN: uncharacterized protein LOC143306047 [Osmia
            lignaria lignaria].
ACCESSION   XP_076548114
VERSION     XP_076548114.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076691999.1
KEYWORDS    RefSeq; corrected model; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio   :: 28% of CDS bases
            frameshifts :: corrected 1 indel
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..560
                     /product="LOW QUALITY PROTEIN: uncharacterized protein
                     LOC143306047"
                     /calculated_mol_wt=64775
     Region          176..239
                     /region_name="HTH_Tnp_Tc5"
                     /note="Tc5 transposase DNA-binding domain; cl12115"
                     /db_xref="CDD:472278"
     CDS             1..560
                     /gene="LOC143306047"
                     /coded_by="XM_076691999.1:1..1683"
                     /note="The sequence of the model RefSeq protein was
                     modified relative to its source genomic sequence to
                     represent the inferred CDS: deleted 1 base in 1 codon"
                     /db_xref="GeneID:143306047"
ORIGIN      
        1 msnvvfvdvd saaqesvcpe edvcndiarl qlkdavvyye ellaqddmia sqpsavindr
       61 amlmgeeiyy rtmemlsekk liecdelfmk depdeenvye eiggysssed yepekktmkt
      121 eyvpleykik vvniatahpn wtlknlqkkg csrlkrmddl akwkahitng gtafdkyaii
      181 dswtndrfqe arknyqqvtt rnlqewtlaa asqfrgfefk asdtwvkqfk rkhgirqrki
      241 tkyvsesett tmeetwasae nfraqvrali pnfnpdfvin taqtgcqyqs tfdrtltrrg
      301 skvvtvqrqs mnkvthsyta qyaltlsgkl lpvvfvclqe asgtfgprvr klveeysaky
      361 qnvvvtrtks gkltsalykv flnnclkpyv qkerflllid swggqtnpvi ydelfqddec
      421 mptcsvkvip pnctplvqpc dvyfyrqvkk iikrlrncsy liqekreins redcikihsi
      481 vhhqlsspif vnmlqyawya sksliqekyi fvnvneicfp vdvlkkacsy mnaafivcar
      541 crnnycfpcf ydkyhtgsch
//
LOCUS       XP_034183018             540 aa            linear   INV 03-SEP-2025
DEFINITION  homeotic protein deformed [Osmia lignaria lignaria].
ACCESSION   XP_034183018
VERSION     XP_034183018.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327127.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..540
                     /product="homeotic protein deformed"
                     /calculated_mol_wt=59277
     Region          325..381
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..540
                     /gene="Dfd"
                     /coded_by="XM_034327127.2:985..2607"
                     /db_xref="GeneID:117605605"
ORIGIN      
        1 mssflmnpsa gggyhhhqhq qaagshhlaa tsvmvdpkfp pseeysqsny ipstgtdffp
       61 gsggghhlnh pqshqlqygy hqhhhqaast pygassavql nggyasyggy ygphhphhqv
      121 havhhaslhp hhhavgalam ppeaqqpplt cpssmqnqqq pqqqqqppvt astvlgstpl
      181 spgimqnhvq ppdnlqhhqq psqqqpphds nacsptntgq mhrdnspdlq qqptttqqsq
      241 hiqntqqqqq qqqqsqqqsq qqhhvddgsd qddmeddqmm dgspgmmede eedeengdrv
      301 iypwmkkihv agvangsyqp gmepkrqrta ytrhqilele kefhynrylt rrrrieiaht
      361 lvlserqiki wfqnrrmkwk kdnklpntkn vrrknangqa apaakpskaq gtrsklttgn
      421 nnsqrknnnn spssgiessl dssvgldmad vhvvnsalph tnvvhpsfqg hphplahlqa
      481 qlshhhvsgi mdgptggplg higsgsispl apatsptgls qvsapipppp iksdygltal
//
LOCUS       XP_076548115             133 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306049 [Osmia lignaria lignaria].
ACCESSION   XP_076548115
VERSION     XP_076548115.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692000.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..133
                     /product="uncharacterized protein LOC143306049"
                     /calculated_mol_wt=15219
     Region          29..>114
                     /region_name="RVT_2"
                     /note="Reverse transcriptase (RNA-dependent DNA
                     polymerase); pfam07727"
                     /db_xref="CDD:400190"
     CDS             1..133
                     /gene="LOC143306049"
                     /coded_by="XM_076692000.1:1..402"
                     /db_xref="GeneID:143306049"
ORIGIN      
        1 mryeeamnst eatqrasaiq nefkaheenk twklvkrtpe mklidskwvf rvktdadgks
       61 hrfkarlcar gflqregidf getfspvvry dslrvllati vaknlevvqf dvqtidgnpe
      121 gaedegrsqe rrv
//
LOCUS       XP_034182885             789 aa            linear   INV 03-SEP-2025
DEFINITION  pseudouridine synthase 7 [Osmia lignaria lignaria].
ACCESSION   XP_034182885
VERSION     XP_034182885.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182885.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..789
                     /product="pseudouridine synthase 7"
                     /calculated_mol_wt=90487
     Region          102..717
                     /region_name="PseudoU_synth_ScPUS7"
                     /note="Pseudouridine synthase, TruD family; cd02576"
                     /db_xref="CDD:211341"
     Site            order(271..274,710)
                     /site_type="active"
                     /note="probable active site [active]"
                     /db_xref="CDD:211341"
     Region          <488..>583
                     /region_name="PHA03151"
                     /note="hypothetical protein; Provisional"
                     /db_xref="CDD:177546"
     CDS             1..789
                     /gene="Pus7"
                     /coded_by="XM_034326994.2:243..2612"
                     /db_xref="GeneID:117605552"
ORIGIN      
        1 maskdidsen nskdtelgrg sepegirekc qkdfkrnwra nndyrrnrgs yhsgyrndnf
       61 grkpfkrnws niqqdskrkk mkvgnrlrec dvgitefign egfsgviker ytdfhvneia
      121 ldgqiakltn qdipvyeeen enledlkisv sdivwdqlqt lkdpgsssie idvtdmdknq
      181 rraihtiakk mtdvvsqtvd kdnrkimmim pskkdssnch tfqrdnrkdw rnrpgeycyf
      241 llhkvnmdtm dalnqlamnl rmrpnnfnya gtkdrrawtt qwvslrkvep sdilraarnv
      301 rgafvgnfkf tkeplklgml cgnhfrialr ningtdeqie itmislrdhg finyyglqrf
      361 gtvvaiptye igktllqgkw neaielilkp regeqdrdlv darkiyettk dayaaykkik
      421 rydkieaall kgiytcgdsn pqgaldlipr narlmyihay qsfvwnhmvs rrikefgtkp
      481 ivgdlvyenn tkqsdndedf vydnenervt ediaesnent nksgdesctd apkedgiiee
      541 stepsspsik eitsatescl kidegseksk ttsekneted lynlpavkil keedlsnytl
      601 advlmpqvgw kvtypsyaks wfdeflakdg lttdlrqknk kyslggtyrk ilqipsnlsw
      661 kimhykekhs dlimcdidem rkstapkdep egqykaliie mslksstyat malreilkyd
      721 tsaqaqaaqs aacnaevens naspdviene nsaeidrnde dekctetqeq dtksidetkd
      781 dkievsnai
//
LOCUS       XP_076548116             733 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306050 [Osmia lignaria lignaria].
ACCESSION   XP_076548116
VERSION     XP_076548116.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692001.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 13% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..733
                     /product="uncharacterized protein LOC143306050"
                     /calculated_mol_wt=82924
     Region          <376..>417
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          498..585
                     /region_name="retropepsin_like"
                     /note="Retropepsins; pepsin-like aspartate proteases;
                     cd00303"
                     /db_xref="CDD:133136"
     Site            order(510,512,514,538..540,581)
                     /site_type="active"
                     /note="inhibitor binding site [active]"
                     /db_xref="CDD:133136"
     Site            510..512
                     /site_type="active"
                     /note="catalytic motif [active]"
                     /db_xref="CDD:133136"
     Site            510
                     /site_type="active"
                     /note="Catalytic residue [active]"
                     /db_xref="CDD:133136"
     Site            order(538..541,546..549)
                     /site_type="active"
                     /note="Active site flap [active]"
                     /db_xref="CDD:133136"
     Region          689..>732
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     CDS             1..733
                     /gene="LOC143306050"
                     /coded_by="XM_076692001.1:1..2202"
                     /db_xref="GeneID:143306050"
ORIGIN      
        1 menleqiatv grkistasaa sikalhrfif nsdgdrsnrk rlrefpgfpf kdgstehvak
       61 lteanaltfg dlvsccnilg ldyrgsreel iqkvcnalmd inslvpahed eevtededpd
      121 ddrrnnqneh engsdpgddr asaasirnee rddnasrqdr prrtvefsmn yrdvedsirp
      181 fdgsgshave rwiadfedia vlfkwsdlqk lvfakkslrg laklyvqseg vvktwtklks
      241 llreefstkv nsaelhnlls qrkmrkdetv qeyylimkei asrgtienda liqyiidgis
      301 detnnklill gtkklsdfkd rlkiyetlrk knqekqiktr edstrrtdfg rkgeyskktt
      361 ftrkqeptta kfeprcyncg dkghlsrdcn rkalgkkcfe cksfghtate crnkekgmlr
      421 gmaaftngpc vrhvsaqsks rsqaptvtlt mkpfihllpg srrecvlekq gkspattdpt
      481 pvvnsistaa khemrkdifi ndvcvkalvd tgscvsllre dvykkigign lhpcttklsg
      541 fgkgnetqpl gyvqviikid seefpttvyi vpvdsmkmeg vigtdiinla elivdpqnit
      601 lrkiplctfl tqmptdsnpl dlghvqddei hdelhgliss ykpdkkkdse ikmriilkee
      661 kpvsqkprrl piserdalqk qidewlengi vepcaseyas pvvivkkkdg sprvcidyrk
      721 lnkitvkdkf plh
//
LOCUS       XP_034182999             935 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605591 [Osmia lignaria lignaria].
ACCESSION   XP_034182999
VERSION     XP_034182999.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327108.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..935
                     /product="uncharacterized protein LOC117605591"
                     /calculated_mol_wt=103649
     Region          <36..>209
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          53..79
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          102..128
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(103..104,107..109,111..112,116,119,128,130,132,
                     138..139,142..144,146..147,151,154,163,165,167,171..172,
                     175..177,179..180,184,187)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          130..163
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          165..195
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          380..>548
                     /region_name="Chb"
                     /note="N-acetyl-beta-hexosaminidase [Carbohydrate
                     transport and metabolism]; COG3525"
                     /db_xref="CDD:442747"
     Region          441..715
                     /region_name="GH20_hexosaminidase"
                     /note="Beta-N-acetylhexosaminidases of glycosyl hydrolase
                     family 20 (GH20) catalyze the removal of beta-1,4-linked
                     N-acetyl-D-hexosamine residues from the non-reducing ends
                     of N-acetyl-beta-D-hexosaminides including
                     N-acetylglucosides and N-acetylgalactosides; cl02948"
                     /db_xref="CDD:445966"
     Site            order(451,524,559..560,625,690,692)
                     /site_type="active"
                     /db_xref="CDD:119331"
     CDS             1..935
                     /gene="LOC117605591"
                     /coded_by="XM_034327108.2:121..2928"
                     /db_xref="GeneID:117605591"
ORIGIN      
        1 mqdicenirt aiehgrtdii rslldaceng nsaegitkek ilnqplleeg tflsyaskan
       61 qpdivrtlls cganpaiqns ngcnavdvas sdairrtyie ellrataase idrviqllda
      121 glnvnswdsh gskntplhwa acygnkdivt yliergadvn aingcgatpl hdavdrgdva
      181 vcqellqaga nplvratkgt fagktpydls rgkqslhcfl qrflpnfvsn eseavhsptt
      241 tfaegnfcqk sissnmsqls idsgksldpv yevpiresgk dsptkstekd giysllwpqp
      301 ktvvelknvs tpfiagkelf isiiqgtesi hkildvweis rthllelgyd vkigevhpss
      361 gkllnenrie civnkklfnk pegyqlhisq ntikvgagsl aglhyavctf vqilrlskih
      421 nrseaceiep ifikdeprft hrgilldisp rgriptleyl lhmidlwssf kisylhlysr
      481 ltpncdwqlc ysksemvtld rycrdrhldl vptldvdsnv gqhhlsqmwp ifqellavfp
      541 slsyvhvgpr lanllvqadn fdlnlsvnet ietdmsevfk sysclqelwh ilnlcpsttl
      601 llcsnglhsk vefhnipnni ilveygfqad ydfsewteaf kiaggnilps sgtasynsla
      661 gcppstyant knaiktsleq dsvgivvahw sgshhltphp fawigyliaa glawnpisei
      721 dmgvddnydm peafgsrqkc itklldihvf qdseykigna ilelgrldtl vltlsknqta
      781 kdlqqipdnr gstlyrlltd pdnvnleyls adlfakvtkq vkrishslye anlsskfgsm
      841 eiqelqltad imltackigr tligvgvnpn snmglavinl gicnlpptfr tdvankmlah
      901 ieqykgswlq rhlpqglqss llvltsalhr fvpet
//
LOCUS       XP_034183000             152 aa            linear   INV 03-SEP-2025
DEFINITION  protein FAM177B [Osmia lignaria lignaria].
ACCESSION   XP_034183000
VERSION     XP_034183000.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327109.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..152
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..152
                     /product="protein FAM177B"
                     /calculated_mol_wt=17552
     Region          28..125
                     /region_name="FAM177"
                     /note="FAM177 family; pfam14774"
                     /db_xref="CDD:434199"
     CDS             1..152
                     /gene="LOC117605592"
                     /coded_by="XM_034327109.2:120..578"
                     /db_xref="GeneID:117605592"
ORIGIN      
        1 mdaendkpsd lshvvlkets nnvahdrksl kqpkrilhfs dgdmeeysed dpdsseenki
       61 thnqidphtl nwlpwtwyqt tlvggkiltg cdyvgewlan ffgitspkye feinefhrlq
      121 alenemlhkq dlemggwnaq nqhnlindnn vq
//
LOCUS       XP_034186733             656 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607298 [Osmia lignaria lignaria].
ACCESSION   XP_034186733
VERSION     XP_034186733.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330842.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186733.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..656
                     /product="uncharacterized protein LOC117607298"
                     /calculated_mol_wt=77129
     Region          68..>108
                     /region_name="DEAD-like_helicase_N"
                     /note="N-terminal helicase domain of the DEAD-box helicase
                     superfamily; cl28899"
                     /db_xref="CDD:475120"
     CDS             1..656
                     /gene="LOC117607298"
                     /coded_by="XM_034330842.2:270..2240"
                     /db_xref="GeneID:117607298"
ORIGIN      
        1 mayevvsieh lklnkyivns irdsdteyqt esdsdtdtsd tdvddtdipd tsldkfqnkn
       61 spkyykcltp pqkkafrqic nlfakknnqv vtinarsgtg ktflltslak hyantvqflt
      121 frrdqasemk fklnnyatty tyisfyvncl hlhyyravql fnvaevddtd llyklilnsk
      181 kylhtgtkvi ivdsctlpsa tmllmlyiis lkhqmhlifa gnklqlsaih kssvhsrcnf
      241 eiirqltnmt irklsdkrlf dkvltnkise frrmiqhgrq knevpflfnl ryylycsfrv
      301 kyfteerfdv iymaqfhqni tdrvnrfiqh levsrvryfi apyctkreaf fepistkqkg
      361 kfypglvlve gykyihinqy gvhsvvllek ilsadnvnvf gldvcfletn qrvrllriel
      421 nyyqilpalr swlvderkqe vklyqfplrp ytltyhaalg ktidndkvel sadcsfanai
      481 yvglccvrny ddiskihain dlpsfvltdy mrkekgeeky yyrcpsqinn feeelkqimq
      541 yvcerrtnaf iesinwrtve niqefekksl kyyaripcsl yekpkkkleq kgdtrlmkiv
      601 dfvknnpnvi ldtianapvg dltkynnkkt qsmelkksgv yvslkekytn wsaree
//
LOCUS       XP_034190757             512 aa            linear   INV 03-SEP-2025
DEFINITION  aromatic-L-amino-acid decarboxylase [Osmia lignaria lignaria].
ACCESSION   XP_034190757
VERSION     XP_034190757.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334866.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190757.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="aromatic-L-amino-acid decarboxylase"
                     /calculated_mol_wt=57514
     Region          35..412
                     /region_name="Pyridoxal_deC"
                     /note="Pyridoxal-dependent decarboxylase conserved domain;
                     pfam00282"
                     /db_xref="CDD:395219"
     Site            order(148..149,152,242,271,274,300,303)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99743"
     Site            303
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99743"
     CDS             1..512
                     /gene="LOC117609029"
                     /coded_by="XM_034334866.2:131..1669"
                     /db_xref="GeneID:117609029"
ORIGIN      
        1 mdtkefvdfg kaaidfvany tdnlrnknvl pdvepgylse llpkeapqka etwqqvlkdv
       61 eqyiipgvth wnsphfhafy ptansypalv geilssgigc iglswlaspa ctelevitmn
      121 wlgkliglpe eflncgegpg ggviqgsase stlvcllvai eqttrrikhl hsdwddayir
      181 sklvaytsdq snssiekagi lasvtmkllp adekcvfrge tllkaikedl ekglvpccvi
      241 atlgttgtca fdkleelgpi ckeynvwlhv daayagaafv cpeyrylmtg veyvdsfnin
      301 phkwllvnfd csalwvkdsr rlievfsvdr iylahdkegv apdyrnwqip lgrrfrslkl
      361 wfvlrlygvd glqehirrti kfaqifeeyv ksdsrfelvi grsmglicfr ikgdnqltke
      421 llsrltaekk iyvvaatfre klvvrfvics rltreedvif awneitkqat eivgpktpcl
      481 eekahesfak ssadlatrie slnlesktqt is
//
LOCUS       XP_076548117             629 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306051 [Osmia lignaria lignaria].
ACCESSION   XP_076548117
VERSION     XP_076548117.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692002.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..629
                     /product="uncharacterized protein LOC143306051"
                     /calculated_mol_wt=69929
     Region          29..245
                     /region_name="RT_nLTR_like"
                     /note="RT_nLTR: Non-LTR (long terminal repeat)
                     retrotransposon and non-LTR retrovirus reverse
                     transcriptase (RT). This subfamily contains both non-LTR
                     retrotransposons and non-LTR retrovirus RTs. RTs catalyze
                     the conversion of single-stranded RNA into...; cd01650"
                     /db_xref="CDD:238827"
     Site            order(104..109,162..163,192,194..195)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238827"
     Site            order(104..109,162,194)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238827"
     Site            163
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238827"
     Region          351..475
                     /region_name="Rnase_HI_RT_non_LTR"
                     /note="non-LTR RNase HI domain of reverse transcriptases;
                     cd09276"
                     /db_xref="CDD:260008"
     Site            order(355..358,383..385,388,414,453,471)
                     /site_type="other"
                     /note="RNA/DNA hybrid binding site [nucleotide binding]"
                     /db_xref="CDD:260008"
     CDS             1..629
                     /gene="LOC143306051"
                     /coded_by="XM_076692002.1:1..1890"
                     /db_xref="GeneID:143306051"
ORIGIN      
        1 mpstrictts messqgglpa qarqkwlrta ksyrpislts fllktlerlv drhvrehtla
       61 krpihasqha yqsgksveta lhhlvrevee alskgksvvs vfidiegafd stsfdvmnea
      121 assfgiddsl itwlgnmlrt rtiitssdgs slrariargc pqggvlspll wtlvvdgllc
      181 rlseegflvl gyaddicisv ksrhqhyiar rlqealtlvq ewclshklkv nptkmemvlf
      241 srkrsfdfka phctngncni lglqkdvwnn vgtktkngqv dlhrnlstpn nirlrcmvdl
      301 heetlqqeep rkdispsiaw ddwgfphhtd hgngdthgpa tttycggsrg qvhshgsktk
      361 tgtgagiwge aqggivavlg hvsilqaevf atwacaklnl erdyrdkhiy icsdskaalg
      421 slrativrsr lvqdctdlle rlagnnnrvn liwvpghagi lgneranmla regarrpgpd
      481 ttyrigvdrg tvkemvrewa rtqtyltwnr tqgmrhakam fkgpssrlgk tlscldrnql
      541 rllvglitgh wytrkhlmhl gtesepgcpr cgeedetplh liwrckglea lrrssfgvpv
      601 lddarvgdig vgrllgfare aglhnlpry
//
LOCUS       XP_034194164             136 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117610648 [Osmia lignaria lignaria].
ACCESSION   XP_034194164
VERSION     XP_034194164.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338273.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194164.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..136
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..136
                     /product="uncharacterized protein LOC117610648"
                     /calculated_mol_wt=15800
     CDS             1..136
                     /gene="LOC117610648"
                     /coded_by="XM_034338273.2:119..529"
                     /db_xref="GeneID:117610648"
ORIGIN      
        1 mvyidpinvf tllsiafnsv dkkvtpvnsa elelkdilyd iimqrmedpt fnieqetyld
       61 ieeefanged pdvnfqwlqe temdltetdl tdeeeqdqql ekctlseapt syeyrknave
      121 ywksgtsnst tchmyk
//
LOCUS       XP_076548118             314 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306052 [Osmia lignaria lignaria].
ACCESSION   XP_076548118
VERSION     XP_076548118.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692003.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..314
                     /product="uncharacterized protein LOC143306052"
                     /calculated_mol_wt=36126
     Region          <18..100
                     /region_name="EEP"
                     /note="Exonuclease-Endonuclease-Phosphatase (EEP) domain
                     superfamily; cl00490"
                     /db_xref="CDD:469791"
     CDS             1..314
                     /gene="LOC143306052"
                     /coded_by="XM_076692003.1:1..945"
                     /db_xref="GeneID:143306052"
ORIGIN      
        1 mlvslaksik tlksnqgiiv mgdfnsrspl wgsdkwcsrg rilfqfcnsv dllptvtkgg
       61 atcdrgsgsk idimicnkla lqdlhdsvvi gdysasdhry llhayhmkqt vvggtspfel
      121 kkgvldeknf vktflnkygd enydkiartd ttlevdrfvd dinkmvnkht rytglsknnr
      181 tpvpwwnnei aekraaanra rrkytrdkar tdhdtieisw kefkrcksel nlvirkfkrd
      241 swatllkevd adiwgrpykl vtrtiknktt rpdvlnekev devvkklfil npenvsdqen
      301 estytkekrg rirt
//
LOCUS       XP_076548119             270 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306053 [Osmia lignaria lignaria].
ACCESSION   XP_076548119
VERSION     XP_076548119.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692004.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..270
                     /product="uncharacterized protein LOC143306053"
                     /calculated_mol_wt=29869
     Region          164..256
                     /region_name="DUF5641"
                     /note="Family of unknown function (DUF5641); pfam18701"
                     /db_xref="CDD:465838"
     CDS             1..270
                     /gene="LOC143306053"
                     /coded_by="XM_076692004.1:1..813"
                     /db_xref="GeneID:143306053"
ORIGIN      
        1 mvtnyttdgf iatyrrfvar rgipstifsd cgtnfigans alksmfvegt qdnqrlaklf
       61 vnvqttwnin psatphmggk weavvksvky hlrrtvsdtl ltyeecttll tqieatlnsr
      121 plgpltddpd dlsvltpahf ligrpistlp epsadhaqls prarwhliqq klqqfwkhws
      181 sqhlqrmqsi skwhhppnni nvgslvlltd ecfppckwpp apvlavhpgp dgltrvvtik
      241 tatttltrpi aklallpvtd tnsspsstsc
//
LOCUS       XP_076548120             715 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306054 [Osmia lignaria lignaria].
ACCESSION   XP_076548120
VERSION     XP_076548120.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692005.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..715
                     /product="uncharacterized protein LOC143306054"
                     /calculated_mol_wt=80285
     Region          291..509
                     /region_name="RT_pepA17"
                     /note="Reverse transcriptase (RTs) in retrotransposons.
                     This subfamily represents the RT domain of a
                     multifunctional enzyme. C-terminal to the RT domain is a
                     domain homologous to aspartic proteinases (corresponding
                     to Merops family A17) encoded by...; cd01644"
                     /db_xref="CDD:238822"
     Site            order(356..361,396..397,432,434..435,500..501)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:238822"
     Site            order(356..361,396,434)
                     /site_type="other"
                     /note="putative NTP binding site [chemical binding]"
                     /db_xref="CDD:238822"
     Site            397
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:238822"
     Region          524..683
                     /region_name="Peptidase_A17"
                     /note="Pao retrotransposon peptidase; pfam05380"
                     /db_xref="CDD:461634"
     CDS             1..715
                     /gene="LOC143306054"
                     /coded_by="XM_076692005.1:1..2148"
                     /db_xref="GeneID:143306054"
ORIGIN      
        1 mttattqnrm amlketylqs qeldakiaag idpktksnmp yfvdnqfeks eeryqetldy
       61 maevlakvaa plepsasnte isnpmtskpt ahlpklqlpt fdgafdewet fqlnladedp
      121 msadpidvii gadlygqlll pgvkkgsayt ptaqntvlgw ilsgpvqssi hvsvginhis
      181 vledldqdlr rfweiealss pilmtpedkn celrasrasn gryivrlpfk teppaklges
      241 rpgalqsfca lerrlsrdta tlrmyrefls eyasfghmkt adplppitrd cyyiphhavf
      301 ressettrlr vvfnasrrss ngkslndlll tgpklqqdlv ailirwrqfq fvytadvekm
      361 yrqilidprd ldyqrilwrd spllpveeyq lltvtygtap apylalrvlq qlaededspy
      421 peavsvlreq syvddflfgg dslesarqlr dqtvellgkg gfslrkwasn ssallsdinp
      481 qnhglatsks lkadeiisvl gitwnpaldq fhfklavpte lpktkrtils tiaklfdplg
      541 wiapviivak iylqtlwssq cdwddvlpss llskwhnfyd qlpdlehisi prkispqiik
      601 tqshgfsdas tkayaaavys rsvlcdgtvs islilaktrv aplktisvpr lelcaallla
      661 rllkfvrial nqetieshcw tdssvtlewv nqpptrwktf vanrvalihe lspna
//
LOCUS       XP_076548121             700 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306055 [Osmia lignaria lignaria].
ACCESSION   XP_076548121
VERSION     XP_076548121.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692006.1
KEYWORDS    RefSeq; includes ab initio.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            
            ##RefSeq-Attributes-START##
            ab initio :: 100% of CDS bases
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..700
                     /product="uncharacterized protein LOC143306055"
                     /calculated_mol_wt=78337
     Region          <2..66
                     /region_name="DUF1759"
                     /note="Protein of unknown function (DUF1759); pfam03564"
                     /db_xref="CDD:281552"
     Region          108..>184
                     /region_name="PTZ00368"
                     /note="universal minicircle sequence binding protein
                     (UMSBP); Provisional"
                     /db_xref="CDD:173561"
     Region          247..399
                     /region_name="pepsin_retropepsin_like"
                     /note="Cellular and retroviral pepsin-like aspartate
                     proteases; cl11403"
                     /db_xref="CDD:472175"
     Region          541..>694
                     /region_name="ps-ssRNAv_RdRp-like"
                     /note="conserved catalytic core domain of RNA-dependent
                     RNA polymerase (RdRp) from the positive-sense
                     single-stranded RNA [(+)ssRNA] viruses and closely related
                     viruses; cl40470"
                     /db_xref="CDD:477363"
     Site            order(605..610,645..646,681,683..684)
                     /site_type="active"
                     /db_xref="CDD:238185"
     Site            order(605..610,645,683)
                     /site_type="other"
                     /note="NTP binding site [chemical binding]"
                     /db_xref="CDD:238185"
     Site            646
                     /site_type="other"
                     /note="nucleic acid binding site [nucleotide binding]"
                     /db_xref="CDD:238185"
     CDS             1..700
                     /gene="LOC143306055"
                     /coded_by="XM_076692006.1:1..2103"
                     /db_xref="GeneID:143306055"
ORIGIN      
        1 mvvsshlrav lelppmkses aielkrvydt indsvhalkn lgravdddfv vaiierkvdv
       61 ktlqewnfll ggstelptft elsqflvnrl ralettqqis egnkksnrtt ntrslvsaid
      121 kskciscnea hslyqctkfk slpvgkrkel vkkhrccfnc lgkghyprnc lssrkcarcg
      181 rkhhsllhed vesnnsdkek aqsdkedegs aansvsptts svalkalisr dlfddsptrv
      241 lpatarvtvr syagrsiklr alidtdleat fiseraaqsl rlsrrkvkir vtgigdqcan
      301 vvnytsqiqi gacgndddfi ktsalilpnr tshqdipnfr fdsiphlrgi kladankskl
      361 dridliigad lygsilldgl rkssadepia qrttlgwivf gpcapsnrqg tpskgisclq
      421 snishandln lrkfweleei psfhaltede nrceqhfast hsrradgryv vklpfkegav
      481 tefvdsfqia srslnrleir lskdvklaea yssflseyeq lghmaptvvs knsefrkstg
      541 yymphhpvlr essttsplrv vfnascvtrs gsslndrllt gpklqsdlps illrwrthrl
      601 vyiadiakmf rqifvypadt kyqrilwrtd vdkpvssydl ltvtygtacg pylamrvlkq
      661 lyddegavfp raisvlhest yvddvlfgah dtseiseiqe
//
LOCUS       XP_034190732             331 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor vestigial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190732
VERSION     XP_034190732.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334841.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190732.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..331
                     /product="transcription factor vestigial isoform X1"
                     /calculated_mol_wt=36972
     Region          155..185
                     /region_name="Vg_Tdu"
                     /note="Vestigial/Tondu family; pfam07545"
                     /db_xref="CDD:462203"
     CDS             1..331
                     /gene="vg"
                     /coded_by="XM_034334841.2:1610..2605"
                     /db_xref="GeneID:117609014"
ORIGIN      
        1 mscsevmyqy ypylyqrppp pphpthphaa phthphtnph aahhsparpa pfqsfsaata
       61 thqydrlnvh qrsleaagle icgaggsvpq gqpssagsvg sapspasprp tpqprpplas
      121 ttsqpsrtld ddrstdavgt gtaaedsddg esraqylnpn cvvfthyrgd aaseveehfq
      181 ralahdklke nispmsmrsf pasfwnsqhp gevyeyptdp whphysqyhh rsriagpgle
      241 rpllvleasy gvswtdlirn irwvhprnnh eldtteplly fdrtastneh cvprksisgk
      301 tlndrrtiee lrryksrskr kieggviiww i
//
LOCUS       XP_034190733             329 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor vestigial isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190733
VERSION     XP_034190733.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334842.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190733.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="transcription factor vestigial isoform X2"
                     /calculated_mol_wt=36802
     Region          153..183
                     /region_name="Vg_Tdu"
                     /note="Vestigial/Tondu family; pfam07545"
                     /db_xref="CDD:462203"
     CDS             1..329
                     /gene="vg"
                     /coded_by="XM_034334842.2:1630..2619"
                     /db_xref="GeneID:117609014"
ORIGIN      
        1 mscsevmyqy ypylyqrppp pphpthphaa phthphtnph aahhsparpa pfqsfsaata
       61 thqydrlnvh qrsleaagle icgaggsvpq gqpssagsvg sapspasprp tpqprpplas
      121 ttsqpsrtld ddrstdgtgt aaedsddges raqylnpncv vfthyrgdaa seveehfqra
      181 lahdklkeni spmsmrsfpa sfwnsqhpge vyeyptdpwh physqyhhrs riagpglerp
      241 llvleasygv swtdlirnir wvhprnnhel dttepllyfd rtastnehcv prksisgktl
      301 ndrrtieelr ryksrskrki eggviiwwi
//
LOCUS       XP_034190734             321 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor vestigial isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190734
VERSION     XP_034190734.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334843.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="transcription factor vestigial isoform X3"
                     /calculated_mol_wt=34875
     Region          155..185
                     /region_name="Vg_Tdu"
                     /note="Vestigial/Tondu family; pfam07545"
                     /db_xref="CDD:462203"
     CDS             1..321
                     /gene="vg"
                     /coded_by="XM_034334843.2:1590..2555"
                     /db_xref="GeneID:117609014"
ORIGIN      
        1 mscsevmyqy ypylyqrppp pphpthphaa phthphtnph aahhsparpa pfqsfsaata
       61 thqydrlnvh qrsleaagle icgaggsvpq gqpssagsvg sapspasprp tpqprpplas
      121 ttsqpsrtld ddrstdavgt gtaaedsddg esraqylnpn cvvfthyrgd aaseveehfq
      181 ralahdklke nispmsmrsf pasfwnsqhp gevyeyptdp whphysqyhh ravheyhhhn
      241 maavtsyggl llggarglgh htshhaahha hpaasykdwp satpsqslvd assappyphg
      301 pyaplsgles qvqdskdlyw f
//
LOCUS       XP_076548122             319 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor vestigial isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548122
VERSION     XP_076548122.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692007.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..319
                     /product="transcription factor vestigial isoform X4"
                     /calculated_mol_wt=34705
     Region          153..183
                     /region_name="Vg_Tdu"
                     /note="Vestigial/Tondu family; pfam07545"
                     /db_xref="CDD:462203"
     CDS             1..319
                     /gene="vg"
                     /coded_by="XM_076692007.1:1610..2569"
                     /db_xref="GeneID:117609014"
ORIGIN      
        1 mscsevmyqy ypylyqrppp pphpthphaa phthphtnph aahhsparpa pfqsfsaata
       61 thqydrlnvh qrsleaagle icgaggsvpq gqpssagsvg sapspasprp tpqprpplas
      121 ttsqpsrtld ddrstdgtgt aaedsddges raqylnpncv vfthyrgdaa seveehfqra
      181 lahdklkeni spmsmrsfpa sfwnsqhpge vyeyptdpwh physqyhhra vheyhhhnma
      241 avtsygglll ggarglghht shhaahhahp aasykdwpsa tpsqslvdas sappyphgpy
      301 aplsglesqv qdskdlywf
//
LOCUS       XP_034190735             278 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor vestigial isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190735
VERSION     XP_034190735.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334844.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190735.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..278
                     /product="transcription factor vestigial isoform X5"
                     /calculated_mol_wt=31212
     CDS             1..278
                     /gene="vg"
                     /coded_by="XM_034334844.2:1684..2520"
                     /db_xref="GeneID:117609014"
ORIGIN      
        1 mscsevmyqy ypylyqrppp pphpthphaa phthphtnph aahhsparpa pfqsfsaata
       61 thqydrlnvh qrsleaagle icgaggsvpq gqpssagsvg sapspasprp tpqprpplas
      121 ttsqpsrtld ddrstdenis pmsmrsfpas fwnsqhpgev yeyptdpwhp hysqyhhrsr
      181 iagpglerpl lvleasygvs wtdlirnirw vhprnnheld ttepllyfdr tastnehcvp
      241 rksisgktln drrtieelrr yksrskrkie ggviiwwi
//
LOCUS       XP_034190736             268 aa            linear   INV 03-SEP-2025
DEFINITION  transcription factor vestigial isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190736
VERSION     XP_034190736.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334845.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="transcription factor vestigial isoform X6"
                     /calculated_mol_wt=29115
     CDS             1..268
                     /gene="vg"
                     /coded_by="XM_034334845.2:1624..2430"
                     /db_xref="GeneID:117609014"
ORIGIN      
        1 mscsevmyqy ypylyqrppp pphpthphaa phthphtnph aahhsparpa pfqsfsaata
       61 thqydrlnvh qrsleaagle icgaggsvpq gqpssagsvg sapspasprp tpqprpplas
      121 ttsqpsrtld ddrstdenis pmsmrsfpas fwnsqhpgev yeyptdpwhp hysqyhhrav
      181 heyhhhnmaa vtsygglllg garglghhts hhaahhahpa asykdwpsat psqslvdass
      241 appyphgpya plsglesqvq dskdlywf
//
LOCUS       XP_034186620            1873 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186620
VERSION     XP_034186620.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330729.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1873
                     /product="dynamin associated protein 160 isoform X1"
                     /calculated_mol_wt=207928
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <90..>407
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1223..1279
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1228,1230,1233,1237,1255..1256,1274,1276..1277)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1306..1358
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1311,1313,1316,1320,1338..1339,1352,1354..1355)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1383..1561
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1580..1736
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1734..1867
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1873
                     /gene="Dap160"
                     /coded_by="XM_034330729.2:383..6004"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1141 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlqtekta eqlederaaa
     1201 edraelpdft amaaqqrgrk peivqviapy qatsseqldl qkgqlimirk ktdsgwwege
     1261 lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt dpnvervmal ypyqaqnede
     1321 lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms nemttdllma gldsmerkrq
     1381 eyikelitte qayiedmrlv hevfekplle slvltvdevd kifvnwrdii acndnflrtl
     1441 rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa vylqrltetv pefvkvahtc
     1501 qqdprtkgmp lssflikpmq ritkypliig kilehtpadh pdrqylqeal akaeefctqv
     1561 negvrekens drlewlqthv acdgleeqli fnsltnslgp rkllhfgilh kaksgkelvg
     1621 fltndfllfa qptvtkksls ggqqfsfern ehqkfkmyrk piflnelsfl gesdtngtvg
     1681 lswgeytens srllrlrdqk kpiillapsp secslwtrrl tearkkfien ektrlqrqrs
     1741 kqaqfgacgr ilvtvlegfn lktipgkcnt fckvsmgsqe ertgvvsgtd cplwdtsmqf
     1801 qvkdlledtl citvfdkgyy spdeflgrae irvadimrds rdscgpiqkr irlhevergi
     1861 vvlkldlilf gnr
//
LOCUS       XP_076548123            1873 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548123
VERSION     XP_076548123.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692008.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1873
                     /product="dynamin associated protein 160 isoform X1"
                     /calculated_mol_wt=207928
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <90..>407
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1223..1279
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1228,1230,1233,1237,1255..1256,1274,1276..1277)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1306..1358
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1311,1313,1316,1320,1338..1339,1352,1354..1355)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1383..1561
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1580..1736
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1734..1867
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1873
                     /gene="Dap160"
                     /coded_by="XM_076692008.1:216..5837"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1141 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlqtekta eqlederaaa
     1201 edraelpdft amaaqqrgrk peivqviapy qatsseqldl qkgqlimirk ktdsgwwege
     1261 lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt dpnvervmal ypyqaqnede
     1321 lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms nemttdllma gldsmerkrq
     1381 eyikelitte qayiedmrlv hevfekplle slvltvdevd kifvnwrdii acndnflrtl
     1441 rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa vylqrltetv pefvkvahtc
     1501 qqdprtkgmp lssflikpmq ritkypliig kilehtpadh pdrqylqeal akaeefctqv
     1561 negvrekens drlewlqthv acdgleeqli fnsltnslgp rkllhfgilh kaksgkelvg
     1621 fltndfllfa qptvtkksls ggqqfsfern ehqkfkmyrk piflnelsfl gesdtngtvg
     1681 lswgeytens srllrlrdqk kpiillapsp secslwtrrl tearkkfien ektrlqrqrs
     1741 kqaqfgacgr ilvtvlegfn lktipgkcnt fckvsmgsqe ertgvvsgtd cplwdtsmqf
     1801 qvkdlledtl citvfdkgyy spdeflgrae irvadimrds rdscgpiqkr irlhevergi
     1861 vvlkldlilf gnr
//
LOCUS       XP_076548124            1873 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548124
VERSION     XP_076548124.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692009.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1873
                     /product="dynamin associated protein 160 isoform X1"
                     /calculated_mol_wt=207928
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <90..>407
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1223..1279
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1228,1230,1233,1237,1255..1256,1274,1276..1277)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1306..1358
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1311,1313,1316,1320,1338..1339,1352,1354..1355)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1383..1561
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1580..1736
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1734..1867
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1873
                     /gene="Dap160"
                     /coded_by="XM_076692009.1:111..5732"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1141 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlqtekta eqlederaaa
     1201 edraelpdft amaaqqrgrk peivqviapy qatsseqldl qkgqlimirk ktdsgwwege
     1261 lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt dpnvervmal ypyqaqnede
     1321 lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms nemttdllma gldsmerkrq
     1381 eyikelitte qayiedmrlv hevfekplle slvltvdevd kifvnwrdii acndnflrtl
     1441 rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa vylqrltetv pefvkvahtc
     1501 qqdprtkgmp lssflikpmq ritkypliig kilehtpadh pdrqylqeal akaeefctqv
     1561 negvrekens drlewlqthv acdgleeqli fnsltnslgp rkllhfgilh kaksgkelvg
     1621 fltndfllfa qptvtkksls ggqqfsfern ehqkfkmyrk piflnelsfl gesdtngtvg
     1681 lswgeytens srllrlrdqk kpiillapsp secslwtrrl tearkkfien ektrlqrqrs
     1741 kqaqfgacgr ilvtvlegfn lktipgkcnt fckvsmgsqe ertgvvsgtd cplwdtsmqf
     1801 qvkdlledtl citvfdkgyy spdeflgrae irvadimrds rdscgpiqkr irlhevergi
     1861 vvlkldlilf gnr
//
LOCUS       XP_076548125            1872 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548125
VERSION     XP_076548125.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692010.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1872
                     /product="dynamin associated protein 160 isoform X2"
                     /calculated_mol_wt=208113
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <90..>407
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1223..1279
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1228,1230,1233,1237,1255..1256,1274,1276..1277)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1306..1358
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1311,1313,1316,1320,1338..1339,1352,1354..1355)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1383..1561
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1580..1732
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1752..1866
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1872
                     /gene="Dap160"
                     /coded_by="XM_076692010.1:383..6001"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1141 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlqtekta eqlederaaa
     1201 edraelpdft amaaqqrgrk peivqviapy qatsseqldl qkgqlimirk ktdsgwwege
     1261 lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt dpnvervmal ypyqaqnede
     1321 lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms nemttdllma gldsmerkrq
     1381 eyikelitte qayiedmrlv hevfekplle slvltvdevd kifvnwrdii acndnflrtl
     1441 rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa vylqrltetv pefvkvahtc
     1501 qqdprtkgmp lssflikpmq ritkypliig kilehtpadh pdrqylqeal akaeefctqv
     1561 negvrekens drlewlqthv acdgleeqli fnsltnslgp rkllhfgilh kaksgkelvg
     1621 fltndfllfa qptvtkksls ggqqfsfern ehqkfkmyrk piflnelsfl gesdtngtvg
     1681 lswgeytens srllrlrdqk kpiillapsp secslwtrrl tearkkfien ektrlqrqrs
     1741 trrrrpegrl rlvvveaedi vikkgkcntf ckvsmgsqee rtgvvsgtdc plwdtsmqfq
     1801 vkdlledtlc itvfdkgyys pdeflgraei rvadimrdsr dscgpiqkri rlhevergiv
     1861 vlkldlilfg nr
//
LOCUS       XP_076548126            1865 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548126
VERSION     XP_076548126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692011.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1865
                     /product="dynamin associated protein 160 isoform X3"
                     /calculated_mol_wt=206995
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <139..406
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          429..524
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          600..>843
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          892..945
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(897,899,902,906,926..927,940,942..943)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1007..1058
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1012,1014,1017,1021,1038..1039,1052,1054..1055)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1074..1125
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1079,1081,1084,1088,1105..1106,1119,1121..1122)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1215..1271
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1220,1222,1225,1229,1247..1248,1266,1268..1269)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1298..1350
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1303,1305,1308,1312,1330..1331,1344,1346..1347)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1375..1553
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1572..1728
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1726..1859
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1865
                     /gene="Dap160"
                     /coded_by="XM_076692011.1:383..5980"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaigmptva piaplntnpt pvaafgmgqt mqmqplatgm iapitstmip svapmpagpg
      361 vvstppnvgl plvsattans tlvngviaqt pvststplst iarppsidrv gsvdsqhsqh
      421 svgspqsvew avphqtklky tqlfntwdrt rsgflsgpqa rnimvqsqlp qkvlaqiwal
      481 admdsdgrls cdefvlamhl cdlvkigeki patlpielip pafrrqrqss ltlsqggten
      541 idpsagmpqt sfedkrkenf ekgqaelerr rkalleiqrk eqeererker eeaekqekir
      601 leqerrrqae iekqmqrqke ieqekeeqrk rvqeqreaar kemerqrqle werqksqelq
      661 tqrqkeqdvl lklkaknqtl tielgtlndk vkelsqkicd trvgvsgvkt tidgmrstrd
      721 aqlqemaalk nklreqnqrl lalsqekari eaknklnmam esagqeaikm afdnkqitlk
      781 qmkdkiadlq qqiearmadi ennngqlqdi ntqltnlitd ckdlyitfed kklkvlelra
      841 ggvtgattdy ttsawgdsaw ndttgsvdds twpvddtttt naveettpgv mkyralyefv
      901 arnqdeisfq pgdiilvppv qntepgwmag eirghtgwfp esyvepvdag sgndnafvqq
      961 dsvekrtleg iaevpenvsd agslggeppi vepiiptlgl giacdlqata lfqyrptteq
     1021 hlpfekgdii kvieqqgdww ygssnngsgw fsktyvkeip psqqpvvvdg lneyymalyp
     1081 yasaepgdln fnqgevilvt kkegdwwtgs iedrvgvfpa nyvekcdaps qfdvcninqd
     1141 dpittnvsei ntdttvttdt ttsihetaas vaqatlqtek taeqledera aaedraelpd
     1201 ftamaaqqrg rkpeivqvia pyqatsseql dlqkgqlimi rkktdsgwwe gelqargkkr
     1261 qigwfpasyv kpltsssnrs tpvshgyqds ptdpnvervm alypyqaqne delsfekgdv
     1321 itvlakqeaa wwkgelngvs gvfpsnyvsp msnemttdll magldsmerk rqeyikelit
     1381 teqayiedmr lvhevfekpl leslvltvde vdkifvnwrd iiacndnflr tlrirrdnse
     1441 ggivrmigdi lceniprmsa yirfcscqis aavylqrlte tvpefvkvah tcqqdprtkg
     1501 mplssflikp mqritkypli igkilehtpa dhpdrqylqe alakaeefct qvnegvreke
     1561 nsdrlewlqt hvacdgleeq lifnsltnsl gprkllhfgi lhkaksgkel vgfltndfll
     1621 faqptvtkks lsggqqfsfe rnehqkfkmy rkpiflnels flgesdtngt vglswgeyte
     1681 nssrllrlrd qkkpiillap spsecslwtr rltearkkfi enektrlqrq rskqaqfgac
     1741 grilvtvleg fnlktipgkc ntfckvsmgs qeertgvvsg tdcplwdtsm qfqvkdlled
     1801 tlcitvfdkg yyspdeflgr aeirvadimr dsrdscgpiq krirlhever givvlkldli
     1861 lfgnr
//
LOCUS       XP_076548127            1865 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548127
VERSION     XP_076548127.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692012.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1865
                     /product="dynamin associated protein 160 isoform X4"
                     /calculated_mol_wt=206994
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <90..>407
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1215..1271
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1220,1222,1225,1229,1247..1248,1266,1268..1269)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1298..1350
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1303,1305,1308,1312,1330..1331,1344,1346..1347)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1375..1553
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1572..1728
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1726..1859
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1865
                     /gene="Dap160"
                     /coded_by="XM_076692012.1:383..5980"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqd
     1141 dpittnvsei ntdttvttdt ttsihetaas vaqatlqtek taeqledera aaedraelpd
     1201 ftamaaqqrg rkpeivqvia pyqatsseql dlqkgqlimi rkktdsgwwe gelqargkkr
     1261 qigwfpasyv kpltsssnrs tpvshgyqds ptdpnvervm alypyqaqne delsfekgdv
     1321 itvlakqeaa wwkgelngvs gvfpsnyvsp msnemttdll magldsmerk rqeyikelit
     1381 teqayiedmr lvhevfekpl leslvltvde vdkifvnwrd iiacndnflr tlrirrdnse
     1441 ggivrmigdi lceniprmsa yirfcscqis aavylqrlte tvpefvkvah tcqqdprtkg
     1501 mplssflikp mqritkypli igkilehtpa dhpdrqylqe alakaeefct qvnegvreke
     1561 nsdrlewlqt hvacdgleeq lifnsltnsl gprkllhfgi lhkaksgkel vgfltndfll
     1621 faqptvtkks lsggqqfsfe rnehqkfkmy rkpiflnels flgesdtngt vglswgeyte
     1681 nssrllrlrd qkkpiillap spsecslwtr rltearkkfi enektrlqrq rskqaqfgac
     1741 grilvtvleg fnlktipgkc ntfckvsmgs qeertgvvsg tdcplwdtsm qfqvkdlled
     1801 tlcitvfdkg yyspdeflgr aeirvadimr dsrdscgpiq krirlhever givvlkldli
     1861 lfgnr
//
LOCUS       XP_076548128            1854 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548128
VERSION     XP_076548128.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692013.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1854
                     /product="dynamin associated protein 160 isoform X5"
                     /calculated_mol_wt=206061
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <139..395
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          418..513
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          589..>832
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          881..934
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(886,888,891,895,915..916,929,931..932)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          996..1047
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1001,1003,1006,1010,1027..1028,1041,1043..1044)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1063..1114
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1068,1070,1073,1077,1094..1095,1108,1110..1111)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1204..1260
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1209,1211,1214,1218,1236..1237,1255,1257..1258)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1287..1339
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1292,1294,1297,1301,1319..1320,1333,1335..1336)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1364..1542
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1561..1717
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1715..1848
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1854
                     /gene="Dap160"
                     /coded_by="XM_076692013.1:383..5947"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgpgv vstppnvglp
      361 lvsattanst lvngviaqtp vststplsti arppsidrvg svdsqhsqhs vgspqsvewa
      421 vphqtklkyt qlfntwdrtr sgflsgpqar nimvqsqlpq kvlaqiwala dmdsdgrlsc
      481 defvlamhlc dlvkigekip atlpielipp afrrqrqssl tlsqggteni dpsagmpqts
      541 fedkrkenfe kgqaelerrr kalleiqrke qeererkere eaekqekirl eqerrrqaei
      601 ekqmqrqkei eqekeeqrkr vqeqreaark emerqrqlew erqksqelqt qrqkeqdvll
      661 klkaknqtlt ielgtlndkv kelsqkicdt rvgvsgvktt idgmrstrda qlqemaalkn
      721 klreqnqrll alsqekarie aknklnmame sagqeaikma fdnkqitlkq mkdkiadlqq
      781 qiearmadie nnngqlqdin tqltnlitdc kdlyitfedk klkvlelrag gvtgattdyt
      841 tsawgdsawn dttgsvddst wpvddttttn aveettpgvm kyralyefva rnqdeisfqp
      901 gdiilvppvq ntepgwmage irghtgwfpe syvepvdags gndnafvqqd svekrtlegi
      961 aevpenvsda gslggeppiv epiiptlglg iacdlqatal fqyrptteqh lpfekgdiik
     1021 vieqqgdwwy gssnngsgwf sktyvkeipp sqqpvvvdgl neyymalypy asaepgdlnf
     1081 nqgevilvtk kegdwwtgsi edrvgvfpan yvekcdapsq fdvcninqdd pittnvsein
     1141 tdttvttdtt tsihetaasv aqatlqtekt aeqlederaa aedraelpdf tamaaqqrgr
     1201 kpeivqviap yqatsseqld lqkgqlimir kktdsgwweg elqargkkrq igwfpasyvk
     1261 pltsssnrst pvshgyqdsp tdpnvervma lypyqaqned elsfekgdvi tvlakqeaaw
     1321 wkgelngvsg vfpsnyvspm snemttdllm agldsmerkr qeyikelitt eqayiedmrl
     1381 vhevfekpll eslvltvdev dkifvnwrdi iacndnflrt lrirrdnseg givrmigdil
     1441 ceniprmsay irfcscqisa avylqrltet vpefvkvaht cqqdprtkgm plssflikpm
     1501 qritkyplii gkilehtpad hpdrqylqea lakaeefctq vnegvreken sdrlewlqth
     1561 vacdgleeql ifnsltnslg prkllhfgil hkaksgkelv gfltndfllf aqptvtkksl
     1621 sggqqfsfer nehqkfkmyr kpiflnelsf lgesdtngtv glswgeyten ssrllrlrdq
     1681 kkpiillaps psecslwtrr ltearkkfie nektrlqrqr skqaqfgacg rilvtvlegf
     1741 nlktipgkcn tfckvsmgsq eertgvvsgt dcplwdtsmq fqvkdlledt lcitvfdkgy
     1801 yspdeflgra eirvadimrd srdscgpiqk rirlheverg ivvlkldlil fgnr
//
LOCUS       XP_076548129            1848 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548129
VERSION     XP_076548129.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692014.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1848
                     /product="dynamin associated protein 160 isoform X6"
                     /calculated_mol_wt=205272
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <139..421
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1223..1279
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1228,1230,1233,1237,1255..1256,1274,1276..1277)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1306..1358
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1311,1313,1316,1320,1338..1339,1352,1354..1355)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1383..1561
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1580..1736
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1731..1842
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1848
                     /gene="Dap160"
                     /coded_by="XM_076692014.1:383..5929"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1141 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlqtekta eqlederaaa
     1201 edraelpdft amaaqqrgrk peivqviapy qatsseqldl qkgqlimirk ktdsgwwege
     1261 lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt dpnvervmal ypyqaqnede
     1321 lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms nemttdllma gldsmerkrq
     1381 eyikelitte qayiedmrlv hevfekplle slvltvdevd kifvnwrdii acndnflrtl
     1441 rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa vylqrltetv pefvkvahtc
     1501 qqdprtkgmp lssflikpmq ritkypliig kilehtpadh pdrqylqeal akaeefctqv
     1561 negvrekens drlewlqthv acdgleeqli fnsltnslgp rkllhfgilh kaksgkelvg
     1621 fltndfllfa qptvtkksls ggqqfsfern ehqkfkmyrk piflnelsfl gesdtngtvg
     1681 lswgeytens srllrlrdqk kpiillapsp secslwtrrl tearkkfien ektrlqrqrs
     1741 skcntfckvs mgsqeertgv vsgtdcplwd tsmqfqvkdl ledtlcitvf dkgyyspdef
     1801 lgraeirvad imrdsrdscg piqkrirlhe vergivvlkl dlilfgnr
//
LOCUS       XP_076548130            1846 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548130
VERSION     XP_076548130.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692015.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1846
                     /product="dynamin associated protein 160 isoform X7"
                     /calculated_mol_wt=205127
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <139..387
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          410..505
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          581..>824
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          873..926
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(878,880,883,887,907..908,921,923..924)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          988..1039
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(993,995,998,1002,1019..1020,1033,1035..1036)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1055..1106
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1060,1062,1065,1069,1086..1087,1100,1102..1103)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1196..1252
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1201,1203,1206,1210,1228..1229,1247,1249..1250)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1279..1331
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1284,1286,1289,1293,1311..1312,1325,1327..1328)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1356..1534
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1553..1709
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1707..1840
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1846
                     /gene="Dap160"
                     /coded_by="XM_076692015.1:383..5923"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaigmptva piaplntnpt pvaafgmgqt mqmqplatgp gvvstppnvg lplvsattan
      361 stlvngviaq tpvststpls tiarppsidr vgsvdsqhsq hsvgspqsve wavphqtklk
      421 ytqlfntwdr trsgflsgpq arnimvqsql pqkvlaqiwa ladmdsdgrl scdefvlamh
      481 lcdlvkigek ipatlpieli ppafrrqrqs sltlsqggte nidpsagmpq tsfedkrken
      541 fekgqaeler rrkalleiqr keqeererke reeaekqeki rleqerrrqa eiekqmqrqk
      601 eieqekeeqr krvqeqreaa rkemerqrql ewerqksqel qtqrqkeqdv llklkaknqt
      661 ltielgtlnd kvkelsqkic dtrvgvsgvk ttidgmrstr daqlqemaal knklreqnqr
      721 llalsqekar ieaknklnma mesagqeaik mafdnkqitl kqmkdkiadl qqqiearmad
      781 iennngqlqd intqltnlit dckdlyitfe dkklkvlelr aggvtgattd yttsawgdsa
      841 wndttgsvdd stwpvddttt tnaveettpg vmkyralyef varnqdeisf qpgdiilvpp
      901 vqntepgwma geirghtgwf pesyvepvda gsgndnafvq qdsvekrtle giaevpenvs
      961 dagslggepp ivepiiptlg lgiacdlqat alfqyrptte qhlpfekgdi ikvieqqgdw
     1021 wygssnngsg wfsktyvkei ppsqqpvvvd glneyymaly pyasaepgdl nfnqgevilv
     1081 tkkegdwwtg siedrvgvfp anyvekcdap sqfdvcninq ddpittnvse intdttvttd
     1141 tttsihetaa svaqatlqte ktaeqleder aaaedraelp dftamaaqqr grkpeivqvi
     1201 apyqatsseq ldlqkgqlim irkktdsgww egelqargkk rqigwfpasy vkpltsssnr
     1261 stpvshgyqd sptdpnverv malypyqaqn edelsfekgd vitvlakqea awwkgelngv
     1321 sgvfpsnyvs pmsnemttdl lmagldsmer krqeyikeli tteqayiedm rlvhevfekp
     1381 lleslvltvd evdkifvnwr diiacndnfl rtlrirrdns eggivrmigd ilceniprms
     1441 ayirfcscqi saavylqrlt etvpefvkva htcqqdprtk gmplssflik pmqritkypl
     1501 iigkilehtp adhpdrqylq ealakaeefc tqvnegvrek ensdrlewlq thvacdglee
     1561 qlifnsltns lgprkllhfg ilhkaksgke lvgfltndfl lfaqptvtkk slsggqqfsf
     1621 ernehqkfkm yrkpiflnel sflgesdtng tvglswgeyt enssrllrlr dqkkpiilla
     1681 pspsecslwt rrltearkkf ienektrlqr qrskqaqfga cgrilvtvle gfnlktipgk
     1741 cntfckvsmg sqeertgvvs gtdcplwdts mqfqvkdlle dtlcitvfdk gyyspdeflg
     1801 raeirvadim rdsrdscgpi qkrirlheve rgivvlkldl ilfgnr
//
LOCUS       XP_076548131            1845 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548131
VERSION     XP_076548131.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692016.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1845
                     /product="dynamin associated protein 160 isoform X8"
                     /calculated_mol_wt=205252
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <90..>379
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          409..504
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          580..>823
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          872..925
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(877,879,882,886,906..907,920,922..923)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          987..1038
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(992,994,997,1001,1018..1019,1032,1034..1035)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1054..1105
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1059,1061,1064,1068,1085..1086,1099,1101..1102)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1195..1251
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1200,1202,1205,1209,1227..1228,1246,1248..1249)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1278..1330
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1283,1285,1288,1292,1310..1311,1324,1326..1327)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1355..1533
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1552..1708
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1706..1839
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1845
                     /gene="Dap160"
                     /coded_by="XM_076692016.1:383..5920"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg anfspvtsip ppkpappsfp
      241 nspvaagmsp mnvppvsaav appvqplqsm atssaidlld felsgmptva piaplntnpt
      301 pvaafgmgqt mqmqplatgm iapitstmip svapmpagpg vvstppnvgl plvsattans
      361 tlvngviaqt pvststplst iarppsidrv gsvdsqhsqh svgspqsvew avphqtklky
      421 tqlfntwdrt rsgflsgpqa rnimvqsqlp qkvlaqiwal admdsdgrls cdefvlamhl
      481 cdlvkigeki patlpielip pafrrqrqss ltlsqggten idpsagmpqt sfedkrkenf
      541 ekgqaelerr rkalleiqrk eqeererker eeaekqekir leqerrrqae iekqmqrqke
      601 ieqekeeqrk rvqeqreaar kemerqrqle werqksqelq tqrqkeqdvl lklkaknqtl
      661 tielgtlndk vkelsqkicd trvgvsgvkt tidgmrstrd aqlqemaalk nklreqnqrl
      721 lalsqekari eaknklnmam esagqeaikm afdnkqitlk qmkdkiadlq qqiearmadi
      781 ennngqlqdi ntqltnlitd ckdlyitfed kklkvlelra ggvtgattdy ttsawgdsaw
      841 ndttgsvdds twpvddtttt naveettpgv mkyralyefv arnqdeisfq pgdiilvppv
      901 qntepgwmag eirghtgwfp esyvepvdag sgndnafvqq dsvekrtleg iaevpenvsd
      961 agslggeppi vepiiptlgl giacdlqata lfqyrptteq hlpfekgdii kvieqqgdww
     1021 ygssnngsgw fsktyvkeip psqqpvvvdg lneyymalyp yasaepgdln fnqgevilvt
     1081 kkegdwwtgs iedrvgvfpa nyvekcdaps qfdvcninqd dpittnvsei ntdttvttdt
     1141 ttsihetaas vaqatlqtek taeqledera aaedraelpd ftamaaqqrg rkpeivqvia
     1201 pyqatsseql dlqkgqlimi rkktdsgwwe gelqargkkr qigwfpasyv kpltsssnrs
     1261 tpvshgyqds ptdpnvervm alypyqaqne delsfekgdv itvlakqeaa wwkgelngvs
     1321 gvfpsnyvsp msnemttdll magldsmerk rqeyikelit teqayiedmr lvhevfekpl
     1381 leslvltvde vdkifvnwrd iiacndnflr tlrirrdnse ggivrmigdi lceniprmsa
     1441 yirfcscqis aavylqrlte tvpefvkvah tcqqdprtkg mplssflikp mqritkypli
     1501 igkilehtpa dhpdrqylqe alakaeefct qvnegvreke nsdrlewlqt hvacdgleeq
     1561 lifnsltnsl gprkllhfgi lhkaksgkel vgfltndfll faqptvtkks lsggqqfsfe
     1621 rnehqkfkmy rkpiflnels flgesdtngt vglswgeyte nssrllrlrd qkkpiillap
     1681 spsecslwtr rltearkkfi enektrlqrq rskqaqfgac grilvtvleg fnlktipgkc
     1741 ntfckvsmgs qeertgvvsg tdcplwdtsm qfqvkdlled tlcitvfdkg yyspdeflgr
     1801 aeirvadimr dsrdscgpiq krirlhever givvlkldli lfgnr
//
LOCUS       XP_076548132            1841 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548132
VERSION     XP_076548132.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692017.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1841
                     /product="dynamin associated protein 160 isoform X9"
                     /calculated_mol_wt=204624
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <136..>375
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          405..500
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          576..>819
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          868..921
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(873,875,878,882,902..903,916,918..919)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          983..1034
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(988,990,993,997,1014..1015,1028,1030..1031)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1050..1101
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1055,1057,1060,1064,1081..1082,1095,1097..1098)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1191..1247
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1196,1198,1201,1205,1223..1224,1242,1244..1245)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1274..1326
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1279,1281,1284,1288,1306..1307,1320,1322..1323)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1351..1529
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1548..1704
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1702..1835
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1841
                     /gene="Dap160"
                     /coded_by="XM_076692017.1:383..5908"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpgiv pststatttt ssggppqrpa pptniganfs pvtsipppkp appsfpnspv
      241 aagmspmnvp pvsaavappv qplqsmatss aidlldfels gmptvapiap lntnptpvaa
      301 fgmgqtmqmq platgmiapi tstmipsvap mpagpgvvst ppnvglplvs attanstlvn
      361 gviaqtpvst stplstiarp psidrvgsvd sqhsqhsvgs pqsvewavph qtklkytqlf
      421 ntwdrtrsgf lsgpqarnim vqsqlpqkvl aqiwaladmd sdgrlscdef vlamhlcdlv
      481 kigekipatl pielippafr rqrqssltls qggtenidps agmpqtsfed krkenfekgq
      541 aelerrrkal leiqrkeqee rerkereeae kqekirleqe rrrqaeiekq mqrqkeieqe
      601 keeqrkrvqe qreaarkeme rqrqlewerq ksqelqtqrq keqdvllklk aknqtltiel
      661 gtlndkvkel sqkicdtrvg vsgvkttidg mrstrdaqlq emaalknklr eqnqrllals
      721 qekarieakn klnmamesag qeaikmafdn kqitlkqmkd kiadlqqqie armadiennn
      781 gqlqdintql tnlitdckdl yitfedkklk vlelraggvt gattdyttsa wgdsawndtt
      841 gsvddstwpv ddttttnave ettpgvmkyr alyefvarnq deisfqpgdi ilvppvqnte
      901 pgwmageirg htgwfpesyv epvdagsgnd nafvqqdsve krtlegiaev penvsdagsl
      961 ggeppivepi iptlglgiac dlqatalfqy rptteqhlpf ekgdiikvie qqgdwwygss
     1021 nngsgwfskt yvkeippsqq pvvvdglney ymalypyasa epgdlnfnqg evilvtkkeg
     1081 dwwtgsiedr vgvfpanyve kcdapsqfdv cninqddpit tnvseintdt tvttdtttsi
     1141 hetaasvaqa tlqtektaeq lederaaaed raelpdftam aaqqrgrkpe ivqviapyqa
     1201 tsseqldlqk gqlimirkkt dsgwwegelq argkkrqigw fpasyvkplt sssnrstpvs
     1261 hgyqdsptdp nvervmalyp yqaqnedels fekgdvitvl akqeaawwkg elngvsgvfp
     1321 snyvspmsne mttdllmagl dsmerkrqey ikelitteqa yiedmrlvhe vfekpllesl
     1381 vltvdevdki fvnwrdiiac ndnflrtlri rrdnseggiv rmigdilcen iprmsayirf
     1441 cscqisaavy lqrltetvpe fvkvahtcqq dprtkgmpls sflikpmqri tkypliigki
     1501 lehtpadhpd rqylqealak aeefctqvne gvrekensdr lewlqthvac dgleeqlifn
     1561 sltnslgprk llhfgilhka ksgkelvgfl tndfllfaqp tvtkkslsgg qqfsferneh
     1621 qkfkmyrkpi flnelsflge sdtngtvgls wgeytenssr llrlrdqkkp iillapspse
     1681 cslwtrrlte arkkfienek trlqrqrskq aqfgacgril vtvlegfnlk tipgkcntfc
     1741 kvsmgsqeer tgvvsgtdcp lwdtsmqfqv kdlledtlci tvfdkgyysp deflgraeir
     1801 vadimrdsrd scgpiqkrir lhevergivv lkldlilfgn r
//
LOCUS       XP_034186630            1841 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186630
VERSION     XP_034186630.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330739.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1841
                     /product="dynamin associated protein 160 isoform X10"
                     /calculated_mol_wt=204381
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <139..421
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1191..1247
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1196,1198,1201,1205,1223..1224,1242,1244..1245)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1274..1326
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1279,1281,1284,1288,1306..1307,1320,1322..1323)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1351..1529
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1548..1704
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1702..1835
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1841
                     /gene="Dap160"
                     /coded_by="XM_034330739.2:383..5908"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1141 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlrgrkpe ivqviapyqa
     1201 tsseqldlqk gqlimirkkt dsgwwegelq argkkrqigw fpasyvkplt sssnrstpvs
     1261 hgyqdsptdp nvervmalyp yqaqnedels fekgdvitvl akqeaawwkg elngvsgvfp
     1321 snyvspmsne mttdllmagl dsmerkrqey ikelitteqa yiedmrlvhe vfekpllesl
     1381 vltvdevdki fvnwrdiiac ndnflrtlri rrdnseggiv rmigdilcen iprmsayirf
     1441 cscqisaavy lqrltetvpe fvkvahtcqq dprtkgmpls sflikpmqri tkypliigki
     1501 lehtpadhpd rqylqealak aeefctqvne gvrekensdr lewlqthvac dgleeqlifn
     1561 sltnslgprk llhfgilhka ksgkelvgfl tndfllfaqp tvtkkslsgg qqfsferneh
     1621 qkfkmyrkpi flnelsflge sdtngtvgls wgeytenssr llrlrdqkkp iillapspse
     1681 cslwtrrlte arkkfienek trlqrqrskq aqfgacgril vtvlegfnlk tipgkcntfc
     1741 kvsmgsqeer tgvvsgtdcp lwdtsmqfqv kdlledtlci tvfdkgyysp deflgraeir
     1801 vadimrdsrd scgpiqkrir lhevergivv lkldlilfgn r
//
LOCUS       XP_034186631            1833 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X11 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186631
VERSION     XP_034186631.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330740.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1833
                     /product="dynamin associated protein 160 isoform X11"
                     /calculated_mol_wt=203447
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <90..>407
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1183..1239
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1188,1190,1193,1197,1215..1216,1234,1236..1237)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1266..1318
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1271,1273,1276,1280,1298..1299,1312,1314..1315)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1343..1521
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1540..1696
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1694..1827
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1833
                     /gene="Dap160"
                     /coded_by="XM_034330740.2:383..5884"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqd
     1141 dpittnvsei ntdttvttdt ttsihetaas vaqatlrgrk peivqviapy qatsseqldl
     1201 qkgqlimirk ktdsgwwege lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt
     1261 dpnvervmal ypyqaqnede lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms
     1321 nemttdllma gldsmerkrq eyikelitte qayiedmrlv hevfekplle slvltvdevd
     1381 kifvnwrdii acndnflrtl rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa
     1441 vylqrltetv pefvkvahtc qqdprtkgmp lssflikpmq ritkypliig kilehtpadh
     1501 pdrqylqeal akaeefctqv negvrekens drlewlqthv acdgleeqli fnsltnslgp
     1561 rkllhfgilh kaksgkelvg fltndfllfa qptvtkksls ggqqfsfern ehqkfkmyrk
     1621 piflnelsfl gesdtngtvg lswgeytens srllrlrdqk kpiillapsp secslwtrrl
     1681 tearkkfien ektrlqrqrs kqaqfgacgr ilvtvlegfn lktipgkcnt fckvsmgsqe
     1741 ertgvvsgtd cplwdtsmqf qvkdlledtl citvfdkgyy spdeflgrae irvadimrds
     1801 rdscgpiqkr irlhevergi vvlkldlilf gnr
//
LOCUS       XP_076548133            1813 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X12 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548133
VERSION     XP_076548133.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692018.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1813
                     /product="dynamin associated protein 160 isoform X12"
                     /calculated_mol_wt=201948
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <136..>347
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          377..472
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          548..>791
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          840..893
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(845,847,850,854,874..875,888,890..891)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          955..1006
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(960,962,965,969,986..987,1000,1002..1003)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1022..1073
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1027,1029,1032,1036,1053..1054,1067,1069..1070)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1163..1219
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1168,1170,1173,1177,1195..1196,1214,1216..1217)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1246..1298
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1251,1253,1256,1260,1278..1279,1292,1294..1295)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1323..1501
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1520..1676
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1674..1807
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1813
                     /gene="Dap160"
                     /coded_by="XM_076692018.1:383..5824"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpgan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      241 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      301 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      361 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      421 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      481 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      541 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      601 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      661 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      721 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      781 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      841 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      901 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
      961 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1021 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1081 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlqtekta eqlederaaa
     1141 edraelpdft amaaqqrgrk peivqviapy qatsseqldl qkgqlimirk ktdsgwwege
     1201 lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt dpnvervmal ypyqaqnede
     1261 lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms nemttdllma gldsmerkrq
     1321 eyikelitte qayiedmrlv hevfekplle slvltvdevd kifvnwrdii acndnflrtl
     1381 rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa vylqrltetv pefvkvahtc
     1441 qqdprtkgmp lssflikpmq ritkypliig kilehtpadh pdrqylqeal akaeefctqv
     1501 negvrekens drlewlqthv acdgleeqli fnsltnslgp rkllhfgilh kaksgkelvg
     1561 fltndfllfa qptvtkksls ggqqfsfern ehqkfkmyrk piflnelsfl gesdtngtvg
     1621 lswgeytens srllrlrdqk kpiillapsp secslwtrrl tearkkfien ektrlqrqrs
     1681 kqaqfgacgr ilvtvlegfn lktipgkcnt fckvsmgsqe ertgvvsgtd cplwdtsmqf
     1741 qvkdlledtl citvfdkgyy spdeflgrae irvadimrds rdscgpiqkr irlhevergi
     1801 vvlkldlilf gnr
//
LOCUS       XP_034186633            1796 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X13 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186633
VERSION     XP_034186633.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330742.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1796
                     /product="dynamin associated protein 160 isoform X13"
                     /calculated_mol_wt=199629
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <139..421
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1146..1202
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1151,1153,1156,1160,1178..1179,1197,1199..1200)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1229..1281
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1234,1236,1239,1243,1261..1262,1275,1277..1278)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1306..1484
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     Region          1503..1659
                     /region_name="PH_13"
                     /note="Pleckstrin homology domain; pfam16652"
                     /db_xref="CDD:465218"
     Region          1657..1790
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     CDS             1..1796
                     /gene="Dap160"
                     /coded_by="XM_034330742.2:383..5773"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqr
     1141 grkpeivqvi apyqatsseq ldlqkgqlim irkktdsgww egelqargkk rqigwfpasy
     1201 vkpltsssnr stpvshgyqd sptdpnverv malypyqaqn edelsfekgd vitvlakqea
     1261 awwkgelngv sgvfpsnyvs pmsnemttdl lmagldsmer krqeyikeli tteqayiedm
     1321 rlvhevfekp lleslvltvd evdkifvnwr diiacndnfl rtlrirrdns eggivrmigd
     1381 ilceniprms ayirfcscqi saavylqrlt etvpefvkva htcqqdprtk gmplssflik
     1441 pmqritkypl iigkilehtp adhpdrqylq ealakaeefc tqvnegvrek ensdrlewlq
     1501 thvacdglee qlifnsltns lgprkllhfg ilhkaksgke lvgfltndfl lfaqptvtkk
     1561 slsggqqfsf ernehqkfkm yrkpiflnel sflgesdtng tvglswgeyt enssrllrlr
     1621 dqkkpiilla pspsecslwt rrltearkkf ienektrlqr qrskqaqfga cgrilvtvle
     1681 gfnlktipgk cntfckvsmg sqeertgvvs gtdcplwdts mqfqvkdlle dtlcitvfdk
     1741 gyyspdeflg raeirvadim rdsrdscgpi qkrirlheve rgivvlkldl ilfgnr
//
LOCUS       XP_076548134            1624 aa            linear   INV 03-SEP-2025
DEFINITION  dynamin associated protein 160 isoform X14 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548134
VERSION     XP_076548134.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692019.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1624
                     /product="dynamin associated protein 160 isoform X14"
                     /calculated_mol_wt=179804
     Region          11..104
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          <139..421
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          437..532
                     /region_name="EH"
                     /note="Eps15 homology domain; smart00027"
                     /db_xref="CDD:197477"
     Region          608..>851
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          900..953
                     /region_name="SH3_Intersectin_1"
                     /note="First Src homology 3 domain (or SH3A) of
                     Intersectin; cd11836"
                     /db_xref="CDD:212770"
     Site            order(905,907,910,914,934..935,948,950..951)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212770"
     Region          1015..1066
                     /region_name="SH3_Intersectin_2"
                     /note="Second Src homology 3 domain (or SH3B) of
                     Intersectin; cd11837"
                     /db_xref="CDD:212771"
     Site            order(1020,1022,1025,1029,1046..1047,1060,1062..1063)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212771"
     Region          1082..1133
                     /region_name="SH3_Intersectin_3"
                     /note="Third Src homology 3 domain (or SH3C) of
                     Intersectin; cd11838"
                     /db_xref="CDD:212772"
     Site            order(1087,1089,1092,1096,1113..1114,1127,1129..1130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212772"
     Region          1223..1279
                     /region_name="SH3_Intersectin_4"
                     /note="Fourth Src homology 3 domain (or SH3D) of
                     Intersectin; cd11839"
                     /db_xref="CDD:212773"
     Site            order(1228,1230,1233,1237,1255..1256,1274,1276..1277)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212773"
     Region          1306..1358
                     /region_name="SH3_Intersectin_5"
                     /note="Fifth Src homology 3 domain (or SH3E) of
                     Intersectin; cd11840"
                     /db_xref="CDD:212774"
     Site            order(1311,1313,1316,1320,1338..1339,1352,1354..1355)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212774"
     Region          1383..1561
                     /region_name="RhoGEF"
                     /note="RhoGEF domain; pfam00621"
                     /db_xref="CDD:459876"
     CDS             1..1624
                     /gene="Dap160"
                     /coded_by="XM_076692019.1:383..5257"
                     /db_xref="GeneID:117607255"
ORIGIN      
        1 matpqtpgmd pwiiqprera ryreqfdslk pingvvtgeq akefllqsql pprilgqiwa
       61 lsdtdadgkm dinefsiack linlklrgye ipkalppslv qslkslsagd anvtnltnga
      121 anipsqnnva slvnlsgmpq vpvqpvqsli ggvsmpgsvp rpligppptn gplpghairp
      181 aspmnlpasr qnrqnvaatt hatthtaskp parpappsvg ivpststatt ttssggppqr
      241 papptnigan fspvtsippp kpappsfpns pvaagmspmn vppvsaavap pvqplqsmat
      301 ssaidlldfe lsgmptvapi aplntnptpv aafgmgqtmq mqplatgmia pitstmipsv
      361 apmpagpgvv stppnvglpl vsattanstl vngviaqtpv ststplstia rppsidrvgs
      421 vdsqhsqhsv gspqsvewav phqtklkytq lfntwdrtrs gflsgpqarn imvqsqlpqk
      481 vlaqiwalad mdsdgrlscd efvlamhlcd lvkigekipa tlpielippa frrqrqsslt
      541 lsqggtenid psagmpqtsf edkrkenfek gqaelerrrk alleiqrkeq eererkeree
      601 aekqekirle qerrrqaeie kqmqrqkeie qekeeqrkrv qeqreaarke merqrqlewe
      661 rqksqelqtq rqkeqdvllk lkaknqtlti elgtlndkvk elsqkicdtr vgvsgvktti
      721 dgmrstrdaq lqemaalknk lreqnqrlla lsqekariea knklnmames agqeaikmaf
      781 dnkqitlkqm kdkiadlqqq iearmadien nngqlqdint qltnlitdck dlyitfedkk
      841 lkvlelragg vtgattdytt sawgdsawnd ttgsvddstw pvddttttna veettpgvmk
      901 yralyefvar nqdeisfqpg diilvppvqn tepgwmagei rghtgwfpes yvepvdagsg
      961 ndnafvqqds vekrtlegia evpenvsdag slggeppive piiptlglgi acdlqatalf
     1021 qyrptteqhl pfekgdiikv ieqqgdwwyg ssnngsgwfs ktyvkeipps qqpvvvdgln
     1081 eyymalypya saepgdlnfn qgevilvtkk egdwwtgsie drvgvfpany vekcdapsqf
     1141 dvcninqddp ittnvseint dttvttdttt sihetaasva qatlqtekta eqlederaaa
     1201 edraelpdft amaaqqrgrk peivqviapy qatsseqldl qkgqlimirk ktdsgwwege
     1261 lqargkkrqi gwfpasyvkp ltsssnrstp vshgyqdspt dpnvervmal ypyqaqnede
     1321 lsfekgdvit vlakqeaaww kgelngvsgv fpsnyvspms nemttdllma gldsmerkrq
     1381 eyikelitte qayiedmrlv hevfekplle slvltvdevd kifvnwrdii acndnflrtl
     1441 rirrdnsegg ivrmigdilc eniprmsayi rfcscqisaa vylqrltetv pefvkvahtc
     1501 qqdprtkgmp lssflikpmq ritkypliig kilehtpadh pdrqylqeal akaeefctqv
     1561 henfftlvys irqkvaknll dslqmifyyl lnrrlrkshy pvdnnshlne tniksskcie
     1621 sqss
//
LOCUS       XP_034186635            1471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186635
VERSION     XP_034186635.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330744.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1471
                     /product="uncharacterized protein LOC117607256 isoform X1"
                     /calculated_mol_wt=159333
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          <187..891
                     /region_name="NACHT"
                     /note="Predicted NTPase, NACHT family domain [Signal
                     transduction mechanisms]; COG5635"
                     /db_xref="CDD:444362"
     Region          562..638
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          605..887
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          745..781
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          783..814
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          817..847
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(849,851,855..856,859..861,863..864,868,871,880,882,
                     884,888..889,892..894,896..897,901,904,913,915,917,
                     921..922,925..927,929..930,934,937)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          849..880
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          869..1142
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          882..913
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          915..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..980
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          982..1014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1016,1018,1022..1023,1026..1028,1030..1031,1035,
                     1039,1048,1050,1052,1056..1057,1060..1062,1064..1065,1069,
                     1072,1081,1083,1085,1089..1090,1093..1095,1097..1098,1102,
                     1105,1114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1016..1048
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1050..1081
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1083..1114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1116..1141
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1471
                     /gene="LOC117607256"
                     /coded_by="XM_034330744.2:1136..5551"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvemllehg alvdcadwdq rtalraaawg ghedivkall qhgadvnrtd degrtaliaa
      721 aymghseive hlldfgaeid hadsdgrtal svaalcvpsn hgyakvvtil lergaavdhq
      781 dkdgmtpllv aafeghrdvc ellleyeadv dhcdatgrtp lwaaasmghg svvalllfwg
      841 cyvdsidneg rtvlsvaaaq ggtdvvkqll drgldeqhrd nsgwtplhya afeghidvce
      901 alleagakid etdndgkgal mlaaqeghat lverlleqhg apidqhahdg ktalrvaale
      961 ghydtvrvll ahnadvnakd adgrstlyil alenrlamar flleharadv esrdsegrtp
     1021 lhvsawqghv emvalllteg gasvnacdne nrtplhsaaw qghaaivrll lehgatpdht
     1081 cnqgatalgi aaqeghehcv rallnhgadp shsdhcgrna ikvaaksghd tvvrlleehs
     1141 anqrslrpgi ngggsssats vtsnstaetk pssailnpls tqyspaespd stkrrscvsl
     1201 gnnssnskss snltgstksd qgkfnqnsmv nqviktplsf tqqlqqcsrg aksrplskll
     1261 splksepqsp iyaspphspl sdslipyspt ntsppsaqta anviqsqlgv slltanqnvp
     1321 ykaqiggynh tsaiyepini kteiidkndi skpfeltnem lglsvakdkk ngldekrnsa
     1381 dthftrdthm riilgnsgag vgrsvkhtse htpasasnak pkrnglvsnp amrlvagvrn
     1441 gienatnrnk pittrvngfq wkaearketp l
//
LOCUS       XP_034186636            1471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186636
VERSION     XP_034186636.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330745.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1471
                     /product="uncharacterized protein LOC117607256 isoform X1"
                     /calculated_mol_wt=159333
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          <187..891
                     /region_name="NACHT"
                     /note="Predicted NTPase, NACHT family domain [Signal
                     transduction mechanisms]; COG5635"
                     /db_xref="CDD:444362"
     Region          562..638
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          605..887
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          745..781
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          783..814
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          817..847
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(849,851,855..856,859..861,863..864,868,871,880,882,
                     884,888..889,892..894,896..897,901,904,913,915,917,
                     921..922,925..927,929..930,934,937)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          849..880
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          869..1142
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          882..913
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          915..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..980
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          982..1014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1016,1018,1022..1023,1026..1028,1030..1031,1035,
                     1039,1048,1050,1052,1056..1057,1060..1062,1064..1065,1069,
                     1072,1081,1083,1085,1089..1090,1093..1095,1097..1098,1102,
                     1105,1114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1016..1048
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1050..1081
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1083..1114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1116..1141
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1471
                     /gene="LOC117607256"
                     /coded_by="XM_034330745.2:1058..5473"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvemllehg alvdcadwdq rtalraaawg ghedivkall qhgadvnrtd degrtaliaa
      721 aymghseive hlldfgaeid hadsdgrtal svaalcvpsn hgyakvvtil lergaavdhq
      781 dkdgmtpllv aafeghrdvc ellleyeadv dhcdatgrtp lwaaasmghg svvalllfwg
      841 cyvdsidneg rtvlsvaaaq ggtdvvkqll drgldeqhrd nsgwtplhya afeghidvce
      901 alleagakid etdndgkgal mlaaqeghat lverlleqhg apidqhahdg ktalrvaale
      961 ghydtvrvll ahnadvnakd adgrstlyil alenrlamar flleharadv esrdsegrtp
     1021 lhvsawqghv emvalllteg gasvnacdne nrtplhsaaw qghaaivrll lehgatpdht
     1081 cnqgatalgi aaqeghehcv rallnhgadp shsdhcgrna ikvaaksghd tvvrlleehs
     1141 anqrslrpgi ngggsssats vtsnstaetk pssailnpls tqyspaespd stkrrscvsl
     1201 gnnssnskss snltgstksd qgkfnqnsmv nqviktplsf tqqlqqcsrg aksrplskll
     1261 splksepqsp iyaspphspl sdslipyspt ntsppsaqta anviqsqlgv slltanqnvp
     1321 ykaqiggynh tsaiyepini kteiidkndi skpfeltnem lglsvakdkk ngldekrnsa
     1381 dthftrdthm riilgnsgag vgrsvkhtse htpasasnak pkrnglvsnp amrlvagvrn
     1441 gienatnrnk pittrvngfq wkaearketp l
//
LOCUS       XP_034186639            1471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186639
VERSION     XP_034186639.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330748.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1471
                     /product="uncharacterized protein LOC117607256 isoform X1"
                     /calculated_mol_wt=159333
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          <187..891
                     /region_name="NACHT"
                     /note="Predicted NTPase, NACHT family domain [Signal
                     transduction mechanisms]; COG5635"
                     /db_xref="CDD:444362"
     Region          562..638
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          605..887
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          745..781
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          783..814
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          817..847
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(849,851,855..856,859..861,863..864,868,871,880,882,
                     884,888..889,892..894,896..897,901,904,913,915,917,
                     921..922,925..927,929..930,934,937)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          849..880
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          869..1142
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          882..913
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          915..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..980
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          982..1014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1016,1018,1022..1023,1026..1028,1030..1031,1035,
                     1039,1048,1050,1052,1056..1057,1060..1062,1064..1065,1069,
                     1072,1081,1083,1085,1089..1090,1093..1095,1097..1098,1102,
                     1105,1114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1016..1048
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1050..1081
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1083..1114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1116..1141
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1471
                     /gene="LOC117607256"
                     /coded_by="XM_034330748.2:1072..5487"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvemllehg alvdcadwdq rtalraaawg ghedivkall qhgadvnrtd degrtaliaa
      721 aymghseive hlldfgaeid hadsdgrtal svaalcvpsn hgyakvvtil lergaavdhq
      781 dkdgmtpllv aafeghrdvc ellleyeadv dhcdatgrtp lwaaasmghg svvalllfwg
      841 cyvdsidneg rtvlsvaaaq ggtdvvkqll drgldeqhrd nsgwtplhya afeghidvce
      901 alleagakid etdndgkgal mlaaqeghat lverlleqhg apidqhahdg ktalrvaale
      961 ghydtvrvll ahnadvnakd adgrstlyil alenrlamar flleharadv esrdsegrtp
     1021 lhvsawqghv emvalllteg gasvnacdne nrtplhsaaw qghaaivrll lehgatpdht
     1081 cnqgatalgi aaqeghehcv rallnhgadp shsdhcgrna ikvaaksghd tvvrlleehs
     1141 anqrslrpgi ngggsssats vtsnstaetk pssailnpls tqyspaespd stkrrscvsl
     1201 gnnssnskss snltgstksd qgkfnqnsmv nqviktplsf tqqlqqcsrg aksrplskll
     1261 splksepqsp iyaspphspl sdslipyspt ntsppsaqta anviqsqlgv slltanqnvp
     1321 ykaqiggynh tsaiyepini kteiidkndi skpfeltnem lglsvakdkk ngldekrnsa
     1381 dthftrdthm riilgnsgag vgrsvkhtse htpasasnak pkrnglvsnp amrlvagvrn
     1441 gienatnrnk pittrvngfq wkaearketp l
//
LOCUS       XP_034186637            1471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186637
VERSION     XP_034186637.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330746.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1471
                     /product="uncharacterized protein LOC117607256 isoform X1"
                     /calculated_mol_wt=159333
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          <187..891
                     /region_name="NACHT"
                     /note="Predicted NTPase, NACHT family domain [Signal
                     transduction mechanisms]; COG5635"
                     /db_xref="CDD:444362"
     Region          562..638
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          605..887
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          745..781
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          783..814
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          817..847
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(849,851,855..856,859..861,863..864,868,871,880,882,
                     884,888..889,892..894,896..897,901,904,913,915,917,
                     921..922,925..927,929..930,934,937)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          849..880
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          869..1142
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          882..913
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          915..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..980
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          982..1014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1016,1018,1022..1023,1026..1028,1030..1031,1035,
                     1039,1048,1050,1052,1056..1057,1060..1062,1064..1065,1069,
                     1072,1081,1083,1085,1089..1090,1093..1095,1097..1098,1102,
                     1105,1114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1016..1048
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1050..1081
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1083..1114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1116..1141
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1471
                     /gene="LOC117607256"
                     /coded_by="XM_034330746.2:1091..5506"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvemllehg alvdcadwdq rtalraaawg ghedivkall qhgadvnrtd degrtaliaa
      721 aymghseive hlldfgaeid hadsdgrtal svaalcvpsn hgyakvvtil lergaavdhq
      781 dkdgmtpllv aafeghrdvc ellleyeadv dhcdatgrtp lwaaasmghg svvalllfwg
      841 cyvdsidneg rtvlsvaaaq ggtdvvkqll drgldeqhrd nsgwtplhya afeghidvce
      901 alleagakid etdndgkgal mlaaqeghat lverlleqhg apidqhahdg ktalrvaale
      961 ghydtvrvll ahnadvnakd adgrstlyil alenrlamar flleharadv esrdsegrtp
     1021 lhvsawqghv emvalllteg gasvnacdne nrtplhsaaw qghaaivrll lehgatpdht
     1081 cnqgatalgi aaqeghehcv rallnhgadp shsdhcgrna ikvaaksghd tvvrlleehs
     1141 anqrslrpgi ngggsssats vtsnstaetk pssailnpls tqyspaespd stkrrscvsl
     1201 gnnssnskss snltgstksd qgkfnqnsmv nqviktplsf tqqlqqcsrg aksrplskll
     1261 splksepqsp iyaspphspl sdslipyspt ntsppsaqta anviqsqlgv slltanqnvp
     1321 ykaqiggynh tsaiyepini kteiidkndi skpfeltnem lglsvakdkk ngldekrnsa
     1381 dthftrdthm riilgnsgag vgrsvkhtse htpasasnak pkrnglvsnp amrlvagvrn
     1441 gienatnrnk pittrvngfq wkaearketp l
//
LOCUS       XP_076548135            1471 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548135
VERSION     XP_076548135.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692020.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1471
                     /product="uncharacterized protein LOC117607256 isoform X1"
                     /calculated_mol_wt=159333
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          <187..891
                     /region_name="NACHT"
                     /note="Predicted NTPase, NACHT family domain [Signal
                     transduction mechanisms]; COG5635"
                     /db_xref="CDD:444362"
     Region          562..638
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          605..887
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          745..781
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          783..814
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          817..847
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(849,851,855..856,859..861,863..864,868,871,880,882,
                     884,888..889,892..894,896..897,901,904,913,915,917,
                     921..922,925..927,929..930,934,937)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          849..880
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          869..1142
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          882..913
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          915..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..980
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          982..1014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1016,1018,1022..1023,1026..1028,1030..1031,1035,
                     1039,1048,1050,1052,1056..1057,1060..1062,1064..1065,1069,
                     1072,1081,1083,1085,1089..1090,1093..1095,1097..1098,1102,
                     1105,1114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1016..1048
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1050..1081
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1083..1114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1116..1141
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1471
                     /gene="LOC117607256"
                     /coded_by="XM_076692020.1:1713..6128"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvemllehg alvdcadwdq rtalraaawg ghedivkall qhgadvnrtd degrtaliaa
      721 aymghseive hlldfgaeid hadsdgrtal svaalcvpsn hgyakvvtil lergaavdhq
      781 dkdgmtpllv aafeghrdvc ellleyeadv dhcdatgrtp lwaaasmghg svvalllfwg
      841 cyvdsidneg rtvlsvaaaq ggtdvvkqll drgldeqhrd nsgwtplhya afeghidvce
      901 alleagakid etdndgkgal mlaaqeghat lverlleqhg apidqhahdg ktalrvaale
      961 ghydtvrvll ahnadvnakd adgrstlyil alenrlamar flleharadv esrdsegrtp
     1021 lhvsawqghv emvalllteg gasvnacdne nrtplhsaaw qghaaivrll lehgatpdht
     1081 cnqgatalgi aaqeghehcv rallnhgadp shsdhcgrna ikvaaksghd tvvrlleehs
     1141 anqrslrpgi ngggsssats vtsnstaetk pssailnpls tqyspaespd stkrrscvsl
     1201 gnnssnskss snltgstksd qgkfnqnsmv nqviktplsf tqqlqqcsrg aksrplskll
     1261 splksepqsp iyaspphspl sdslipyspt ntsppsaqta anviqsqlgv slltanqnvp
     1321 ykaqiggynh tsaiyepini kteiidkndi skpfeltnem lglsvakdkk ngldekrnsa
     1381 dthftrdthm riilgnsgag vgrsvkhtse htpasasnak pkrnglvsnp amrlvagvrn
     1441 gienatnrnk pittrvngfq wkaearketp l
//
LOCUS       XP_034186640            1468 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186640
VERSION     XP_034186640.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330749.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1468
                     /product="uncharacterized protein LOC117607256 isoform X2"
                     /calculated_mol_wt=158992
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          <187..891
                     /region_name="NACHT"
                     /note="Predicted NTPase, NACHT family domain [Signal
                     transduction mechanisms]; COG5635"
                     /db_xref="CDD:444362"
     Region          562..638
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          605..887
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          745..781
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          783..814
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          817..847
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(849,851,855..856,859..861,863..864,868,871,880,882,
                     884,888..889,892..894,896..897,901,904,913,915,917,
                     921..922,925..927,929..930,934,937)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          849..880
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          869..1142
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          882..913
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          915..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..980
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          982..1014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1016,1018,1022..1023,1026..1028,1030..1031,1035,
                     1039,1048,1050,1052,1056..1057,1060..1062,1064..1065,1069,
                     1072,1081,1083,1085,1089..1090,1093..1095,1097..1098,1102,
                     1105,1114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1016..1048
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1050..1081
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1083..1114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1116..1141
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1468
                     /gene="LOC117607256"
                     /coded_by="XM_034330749.2:1136..5542"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvemllehg alvdcadwdq rtalraaawg ghedivkall qhgadvnrtd degrtaliaa
      721 aymghseive hlldfgaeid hadsdgrtal svaalcvpsn hgyakvvtil lergaavdhq
      781 dkdgmtpllv aafeghrdvc ellleyeadv dhcdatgrtp lwaaasmghg svvalllfwg
      841 cyvdsidneg rtvlsvaaaq ggtdvvkqll drgldeqhrd nsgwtplhya afeghidvce
      901 alleagakid etdndgkgal mlaaqeghat lverlleqhg apidqhahdg ktalrvaale
      961 ghydtvrvll ahnadvnakd adgrstlyil alenrlamar flleharadv esrdsegrtp
     1021 lhvsawqghv emvalllteg gasvnacdne nrtplhsaaw qghaaivrll lehgatpdht
     1081 cnqgatalgi aaqeghehcv rallnhgadp shsdhcgrna ikvaaksghd tvvrlleehs
     1141 anqrslrpgi ngggsssats vtsnstaetk pssailnpls tqyspaespd stkrrscvsl
     1201 gnnssnskss snltgstksd qgkfnqnsmv nqtplsftqq lqqcsrgaks rplskllspl
     1261 ksepqspiya spphsplsds lipysptnts ppsaqtaanv iqsqlgvsll tanqnvpyka
     1321 qiggynhtsa iyepinikte iidkndiskp feltnemlgl svakdkkngl dekrnsadth
     1381 ftrdthmrii lgnsgagvgr svkhtsehtp asasnakpkr nglvsnpamr lvagvrngie
     1441 natnrnkpit trvngfqwka earketpl
//
LOCUS       XP_034186641            1367 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186641
VERSION     XP_034186641.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330750.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1367
                     /product="uncharacterized protein LOC117607256 isoform X3"
                     /calculated_mol_wt=148233
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          583..883
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          713..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(745,747,751..752,755..757,759..760,764,767,776,778,
                     780,784..785,788..790,792..793,797,800,809,811,813,
                     817..818,821..823,825..826,830,833)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          745..776
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          765..1038
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          778..809
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          811..840
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          845..876
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          878..910
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(912,914,918..919,922..924,926..927,931,935,944,946,
                     948,952..953,956..958,960..961,965,968,977,979,981,
                     985..986,989..991,993..994,998,1001,1010)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          912..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          946..977
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          979..1010
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1012..1037
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1367
                     /gene="LOC117607256"
                     /coded_by="XM_034330750.2:1136..5239"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvtillerg aavdhqdkdg mtpllvaafe ghrdvcelll eyeadvdhcd atgrtplwaa
      721 asmghgsvva lllfwgcyvd sidnegrtvl svaaaqggtd vvkqlldrgl deqhrdnsgw
      781 tplhyaafeg hidvcealle agakidetdn dgkgalmlaa qeghatlver lleqhgapid
      841 qhahdgktal rvaaleghyd tvrvllahna dvnakdadgr stlyilalen rlamarflle
      901 haradvesrd segrtplhvs awqghvemva lllteggasv nacdnenrtp lhsaawqgha
      961 aivrlllehg atpdhtcnqg atalgiaaqe ghehcvrall nhgadpshsd hcgrnaikva
     1021 aksghdtvvr lleehsanqr slrpginggg sssatsvtsn staetkpssa ilnplstqys
     1081 paespdstkr rscvslgnns snsksssnlt gstksdqgkf nqnsmvnqvi ktplsftqql
     1141 qqcsrgaksr plskllsplk sepqspiyas pphsplsdsl ipysptntsp psaqtaanvi
     1201 qsqlgvsllt anqnvpykaq iggynhtsai yepiniktei idkndiskpf eltnemlgls
     1261 vakdkkngld ekrnsadthf trdthmriil gnsgagvgrs vkhtsehtpa sasnakpkrn
     1321 glvsnpamrl vagvrngien atnrnkpitt rvngfqwkae arketpl
//
LOCUS       XP_034186642            1283 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607256 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186642
VERSION     XP_034186642.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330751.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1283
                     /product="uncharacterized protein LOC117607256 isoform X4"
                     /calculated_mol_wt=139437
     Region          10..>108
                     /region_name="AAA_16"
                     /note="AAA ATPase domain; pfam13191"
                     /db_xref="CDD:433025"
     Region          <187..891
                     /region_name="NACHT"
                     /note="Predicted NTPase, NACHT family domain [Signal
                     transduction mechanisms]; COG5635"
                     /db_xref="CDD:444362"
     Region          562..638
                     /region_name="Ank_2"
                     /note="Ankyrin repeats (3 copies); pfam12796"
                     /db_xref="CDD:463710"
     Region          579..611
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          605..887
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Site            order(613,615,619..620,623..625,627..628,632,635,644,646,
                     648,652..653,656..658,660..661,665,668,677,679,681,
                     685..686,689..691,693..694,698,701,710)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          613..644
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          646..677
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          679..710
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          712..743
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          745..781
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          783..814
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          817..847
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(849,851,855..856,859..861,863..864,868,871,880,882,
                     884,888..889,892..894,896..897,901,904,913,915,917,
                     921..922,925..927,929..930,934,937)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          849..880
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          869..1142
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          882..913
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          915..944
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          949..980
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          982..1014
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(1016,1018,1022..1023,1026..1028,1030..1031,1035,
                     1039,1048,1050,1052,1056..1057,1060..1062,1064..1065,1069,
                     1072,1081,1083,1085,1089..1090,1093..1095,1097..1098,1102,
                     1105,1114)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          1016..1048
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1050..1081
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1083..1114
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          1116..1141
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..1283
                     /gene="LOC117607256"
                     /coded_by="XM_034330751.2:1137..4988"
                     /db_xref="GeneID:117607256"
ORIGIN      
        1 maapviekkr ffcrewafik lshcleqrpa sktcgalivg gpgsgktalc aelawpssga
       61 narhqrslnr rllarhfcqa rseaslspaq fvrslvaqll qassdgihra spssptpsst
      121 aattnttttv pltsreavae ayaeklrtdp diqaalqpdv ldrdpddalk kallipllev
      181 eppksclfll vdsidegqtl nppqtgtrds rrenenvsrt iaellanhhh lfpqwlllvc
      241 tarrqsktis rmftgfrkis lddlrksqvv rdvqqyilar ldqedalrqh isrdtaemln
      301 qlhiksngcf lylekvldgv aenfivlrev reipgtlngl ylwlcqrlfs rkqfakvqpl
      361 lnvilaaklp itqeilykcv ktacvsitie dfnrrlhllr rvisvsraga lmlfhhsfae
      421 wlldvkhctq kylcsavegh amlaayytlr gpdlnpdeic vlgqhlqrai tsvattncnl
      481 dvhtlqvlwm igsgapieec yldssecilw prqeakllkl lidagakpse kvveddtnkd
      541 avsssqvsqd vspmdespse pltellgesg dinqadscgr tvlhtlaadg nasllelala
      601 tcpqakleat drhgqtplnl aarhgyadvv rvllaagaca dhadcdgwta lraaawgght
      661 qvvemllehg alvdcadwdq rtalraaawg ghedivkall qhgadvnrtd degrtaliaa
      721 aymghseive hlldfgaeid hadsdgrtal svaalcvpsn hgyakvvtil lergaavdhq
      781 dkdgmtpllv aafeghrdvc ellleyeadv dhcdatgrtp lwaaasmghg svvalllfwg
      841 cyvdsidneg rtvlsvaaaq ggtdvvkqll drgldeqhrd nsgwtplhya afeghidvce
      901 alleagakid etdndgkgal mlaaqeghat lverlleqhg apidqhahdg ktalrvaale
      961 ghydtvrvll ahnadvnakd adgrstlyil alenrlamar flleharadv esrdsegrtp
     1021 lhvsawqghv emvalllteg gasvnacdne nrtplhsaaw qghaaivrll lehgatpdht
     1081 cnqgatalgi aaqeghehcv rallnhgadp shsdhcgrna ikvaaksghd tvvrlleehs
     1141 anqrslrpgi ngggsssats vtsnstaetk pssailnpls tqyspaespd stkrrscvsl
     1201 gnnssnskss snltgstksd qgkfnqnsmv nqvikavlse pppqqdtgir ycgwrivitk
     1261 krksinkffs fylskiklph ikk
//
LOCUS       XP_076548136            1328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607257 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548136
VERSION     XP_076548136.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692021.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1328
                     /product="uncharacterized protein LOC117607257 isoform X1"
                     /calculated_mol_wt=146931
     CDS             1..1328
                     /gene="LOC117607257"
                     /coded_by="XM_076692021.1:1787..5773"
                     /db_xref="GeneID:117607257"
ORIGIN      
        1 mrnplgvgtr frsmkdvptc pclsfhymtr kmqpnpwtlm ialafvasvs seylmgghmd
       61 ngppksllee messptlart aqlddldldl daegstvals tsndeeapry hlpypfafng
      121 grpfslekdp itgkidfeka ppvkalnyts ryqvpvndee qpddkefsne dtkknaenke
      181 tvdvspnein pyspnfhdfl nlpvhyssdk ygkdkyplis ssyantkvqs gsnsystynh
      241 kpyhgetvay yhtrkpyvsk ttsatttttn ttprttssst tttttttttp kpvttstttt
      301 ttttttttpp ppsstasvst kapvvttwkp psttkrfvgq lddyedilpi eklqatmynn
      361 rhqgtnnimh nrdpqtkpsm qheeymdsye dyemndgddy vpvkdstnei vktstlpatt
      421 stisnvtgtt vgtpetpvst tavtstatps assthsssvl qentyqtnsv pfqalpqrpv
      481 tsmpldndpr fpsgfendnr kqgiadnviv nqnyrpnpiv gqrpsglsgg yvvestsniv
      541 ippdqdtvsf vlgnrqnveg gyysvgtaig enpyvgspgi dtsfrplygv psdkgnydpq
      601 tdhlgtiglp svsvlennpn yapqawrqpn psasqklgne iepsrpqnsf vkgtvlmeqa
      661 ddkkddkdvn fvvfpkdepk qpveehivvi neadgtvhei tassttkkpv rvnppvlnee
      721 hlpqlsedlt ppterprppp qfhyhyhhgg tarpdhprpq rlpipprgkq ppspafpppp
      781 ppseavgirr rpyspdtnlp nilpqfrpna ktshghrgse sigtipagqt ypstrirqpv
      841 qshpptrrpl ppppsylqrl npppppihal rlaaaastkt envipprepe ptvkrfrppp
      901 ipatsrgedd vklaqrlssq klrleeeers eryseeppql pprppifpkr rtadpphvat
      961 lqmiqhhgef eedntesnps stgtntndes eriltdqdad rrkfdereti aeppvyvvyp
     1021 vntavnihpd dsrekdesvv vgtrgphrpl ppetllqdne eqemneesgp ivqnmfnsrp
     1081 vasdfpyple rpdpsilvtg mretpllvps dqrqedstke sneekdekds svnvipylqd
     1141 fvpfparkne visatlyrgp sppsstpiaf vytptaqash rldvdvrdee dnskseknep
     1201 kpvllpsqqp ssssssapsp qnfmapfvas vsaeapskng wsvvmvepaa nrksdedrds
     1261 lenpsdtees qteksefdve nfkpqlfggf kpiyefptqd edraerresg ddnseavtsv
     1321 qqsvnstv
//
LOCUS       XP_076548137            1328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607257 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548137
VERSION     XP_076548137.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692022.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1328
                     /product="uncharacterized protein LOC117607257 isoform X1"
                     /calculated_mol_wt=146931
     CDS             1..1328
                     /gene="LOC117607257"
                     /coded_by="XM_076692022.1:1893..5879"
                     /db_xref="GeneID:117607257"
ORIGIN      
        1 mrnplgvgtr frsmkdvptc pclsfhymtr kmqpnpwtlm ialafvasvs seylmgghmd
       61 ngppksllee messptlart aqlddldldl daegstvals tsndeeapry hlpypfafng
      121 grpfslekdp itgkidfeka ppvkalnyts ryqvpvndee qpddkefsne dtkknaenke
      181 tvdvspnein pyspnfhdfl nlpvhyssdk ygkdkyplis ssyantkvqs gsnsystynh
      241 kpyhgetvay yhtrkpyvsk ttsatttttn ttprttssst tttttttttp kpvttstttt
      301 ttttttttpp ppsstasvst kapvvttwkp psttkrfvgq lddyedilpi eklqatmynn
      361 rhqgtnnimh nrdpqtkpsm qheeymdsye dyemndgddy vpvkdstnei vktstlpatt
      421 stisnvtgtt vgtpetpvst tavtstatps assthsssvl qentyqtnsv pfqalpqrpv
      481 tsmpldndpr fpsgfendnr kqgiadnviv nqnyrpnpiv gqrpsglsgg yvvestsniv
      541 ippdqdtvsf vlgnrqnveg gyysvgtaig enpyvgspgi dtsfrplygv psdkgnydpq
      601 tdhlgtiglp svsvlennpn yapqawrqpn psasqklgne iepsrpqnsf vkgtvlmeqa
      661 ddkkddkdvn fvvfpkdepk qpveehivvi neadgtvhei tassttkkpv rvnppvlnee
      721 hlpqlsedlt ppterprppp qfhyhyhhgg tarpdhprpq rlpipprgkq ppspafpppp
      781 ppseavgirr rpyspdtnlp nilpqfrpna ktshghrgse sigtipagqt ypstrirqpv
      841 qshpptrrpl ppppsylqrl npppppihal rlaaaastkt envipprepe ptvkrfrppp
      901 ipatsrgedd vklaqrlssq klrleeeers eryseeppql pprppifpkr rtadpphvat
      961 lqmiqhhgef eedntesnps stgtntndes eriltdqdad rrkfdereti aeppvyvvyp
     1021 vntavnihpd dsrekdesvv vgtrgphrpl ppetllqdne eqemneesgp ivqnmfnsrp
     1081 vasdfpyple rpdpsilvtg mretpllvps dqrqedstke sneekdekds svnvipylqd
     1141 fvpfparkne visatlyrgp sppsstpiaf vytptaqash rldvdvrdee dnskseknep
     1201 kpvllpsqqp ssssssapsp qnfmapfvas vsaeapskng wsvvmvepaa nrksdedrds
     1261 lenpsdtees qteksefdve nfkpqlfggf kpiyefptqd edraerresg ddnseavtsv
     1321 qqsvnstv
//
LOCUS       XP_034186643            1328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607257 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186643
VERSION     XP_034186643.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330752.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186643.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1328
                     /product="uncharacterized protein LOC117607257 isoform X1"
                     /calculated_mol_wt=146931
     CDS             1..1328
                     /gene="LOC117607257"
                     /coded_by="XM_034330752.2:722..4708"
                     /db_xref="GeneID:117607257"
ORIGIN      
        1 mrnplgvgtr frsmkdvptc pclsfhymtr kmqpnpwtlm ialafvasvs seylmgghmd
       61 ngppksllee messptlart aqlddldldl daegstvals tsndeeapry hlpypfafng
      121 grpfslekdp itgkidfeka ppvkalnyts ryqvpvndee qpddkefsne dtkknaenke
      181 tvdvspnein pyspnfhdfl nlpvhyssdk ygkdkyplis ssyantkvqs gsnsystynh
      241 kpyhgetvay yhtrkpyvsk ttsatttttn ttprttssst tttttttttp kpvttstttt
      301 ttttttttpp ppsstasvst kapvvttwkp psttkrfvgq lddyedilpi eklqatmynn
      361 rhqgtnnimh nrdpqtkpsm qheeymdsye dyemndgddy vpvkdstnei vktstlpatt
      421 stisnvtgtt vgtpetpvst tavtstatps assthsssvl qentyqtnsv pfqalpqrpv
      481 tsmpldndpr fpsgfendnr kqgiadnviv nqnyrpnpiv gqrpsglsgg yvvestsniv
      541 ippdqdtvsf vlgnrqnveg gyysvgtaig enpyvgspgi dtsfrplygv psdkgnydpq
      601 tdhlgtiglp svsvlennpn yapqawrqpn psasqklgne iepsrpqnsf vkgtvlmeqa
      661 ddkkddkdvn fvvfpkdepk qpveehivvi neadgtvhei tassttkkpv rvnppvlnee
      721 hlpqlsedlt ppterprppp qfhyhyhhgg tarpdhprpq rlpipprgkq ppspafpppp
      781 ppseavgirr rpyspdtnlp nilpqfrpna ktshghrgse sigtipagqt ypstrirqpv
      841 qshpptrrpl ppppsylqrl npppppihal rlaaaastkt envipprepe ptvkrfrppp
      901 ipatsrgedd vklaqrlssq klrleeeers eryseeppql pprppifpkr rtadpphvat
      961 lqmiqhhgef eedntesnps stgtntndes eriltdqdad rrkfdereti aeppvyvvyp
     1021 vntavnihpd dsrekdesvv vgtrgphrpl ppetllqdne eqemneesgp ivqnmfnsrp
     1081 vasdfpyple rpdpsilvtg mretpllvps dqrqedstke sneekdekds svnvipylqd
     1141 fvpfparkne visatlyrgp sppsstpiaf vytptaqash rldvdvrdee dnskseknep
     1201 kpvllpsqqp ssssssapsp qnfmapfvas vsaeapskng wsvvmvepaa nrksdedrds
     1261 lenpsdtees qteksefdve nfkpqlfggf kpiyefptqd edraerresg ddnseavtsv
     1321 qqsvnstv
//
LOCUS       XP_076548138            1301 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607257 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548138
VERSION     XP_076548138.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692023.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1301
                     /product="uncharacterized protein LOC117607257 isoform X2"
                     /calculated_mol_wt=143836
     CDS             1..1301
                     /gene="LOC117607257"
                     /coded_by="XM_076692023.1:876..4781"
                     /db_xref="GeneID:117607257"
ORIGIN      
        1 mtrkmqpnpw tlmialafva svsseylmgg hmdngppksl leemessptl artaqlddld
       61 ldldaegstv alstsndeea pryhlpypfa fnggrpfsle kdpitgkidf ekappvkaln
      121 ytsryqvpvn deeqpddkef snedtkknae nketvdvspn einpyspnfh dflnlpvhys
      181 sdkygkdkyp lisssyantk vqsgsnsyst ynhkpyhget vayyhtrkpy vskttsattt
      241 ttnttprtts sstttttttt ttpkpvttst tttttttttt tppppsstas vstkapvvtt
      301 wkppsttkrf vgqlddyedi lpieklqatm ynnrhqgtnn imhnrdpqtk psmqheeymd
      361 syedyemndg ddyvpvkdst neivktstlp attstisnvt gttvgtpetp vsttavtsta
      421 tpsassthss svlqentyqt nsvpfqalpq rpvtsmpldn dprfpsgfen dnrkqgiadn
      481 vivnqnyrpn pivgqrpsgl sggyvvests nivippdqdt vsfvlgnrqn veggyysvgt
      541 aigenpyvgs pgidtsfrpl ygvpsdkgny dpqtdhlgti glpsvsvlen npnyapqawr
      601 qpnpsasqkl gneiepsrpq nsfvkgtvlm eqaddkkddk dvnfvvfpkd epkqpveehi
      661 vvineadgtv heitassttk kpvrvnppvl neehlpqlse dltppterpr pppqfhyhyh
      721 hggtarpdhp rpqrlpippr gkqppspafp pppppseavg irrrpyspdt nlpnilpqfr
      781 pnaktshghr gsesigtipa gqtypstrir qpvqshpptr rplppppsyl qrlnpppppi
      841 halrlaaaas tktenvippr epeptvkrfr pppipatsrg eddvklaqrl ssqklrleee
      901 erseryseep pqlpprppif pkrrtadpph vatlqmiqhh gefeedntes npsstgtntn
      961 deseriltdq dadrrkfder etiaeppvyv vypvntavni hpddsrekde svvvgtrgph
     1021 rplppetllq dneeqemnee sgpivqnmfn srpvasdfpy plerpdpsil vtgmretpll
     1081 vpsdqrqeds tkesneekde kdssvnvipy lqdfvpfpar knevisatly rgpsppsstp
     1141 iafvytptaq ashrldvdvr deednsksek nepkpvllps qqpssssssa pspqnfmapf
     1201 vasvsaeaps kngwsvvmve paanrksded rdslenpsdt eesqteksef dvenfkpqlf
     1261 ggfkpiyefp tqdedraerr esgddnseav tsvqqsvnst v
//
LOCUS       XP_076548139            1301 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607257 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548139
VERSION     XP_076548139.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692024.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1301
                     /product="uncharacterized protein LOC117607257 isoform X2"
                     /calculated_mol_wt=143836
     CDS             1..1301
                     /gene="LOC117607257"
                     /coded_by="XM_076692024.1:1256..5161"
                     /db_xref="GeneID:117607257"
ORIGIN      
        1 mtrkmqpnpw tlmialafva svsseylmgg hmdngppksl leemessptl artaqlddld
       61 ldldaegstv alstsndeea pryhlpypfa fnggrpfsle kdpitgkidf ekappvkaln
      121 ytsryqvpvn deeqpddkef snedtkknae nketvdvspn einpyspnfh dflnlpvhys
      181 sdkygkdkyp lisssyantk vqsgsnsyst ynhkpyhget vayyhtrkpy vskttsattt
      241 ttnttprtts sstttttttt ttpkpvttst tttttttttt tppppsstas vstkapvvtt
      301 wkppsttkrf vgqlddyedi lpieklqatm ynnrhqgtnn imhnrdpqtk psmqheeymd
      361 syedyemndg ddyvpvkdst neivktstlp attstisnvt gttvgtpetp vsttavtsta
      421 tpsassthss svlqentyqt nsvpfqalpq rpvtsmpldn dprfpsgfen dnrkqgiadn
      481 vivnqnyrpn pivgqrpsgl sggyvvests nivippdqdt vsfvlgnrqn veggyysvgt
      541 aigenpyvgs pgidtsfrpl ygvpsdkgny dpqtdhlgti glpsvsvlen npnyapqawr
      601 qpnpsasqkl gneiepsrpq nsfvkgtvlm eqaddkkddk dvnfvvfpkd epkqpveehi
      661 vvineadgtv heitassttk kpvrvnppvl neehlpqlse dltppterpr pppqfhyhyh
      721 hggtarpdhp rpqrlpippr gkqppspafp pppppseavg irrrpyspdt nlpnilpqfr
      781 pnaktshghr gsesigtipa gqtypstrir qpvqshpptr rplppppsyl qrlnpppppi
      841 halrlaaaas tktenvippr epeptvkrfr pppipatsrg eddvklaqrl ssqklrleee
      901 erseryseep pqlpprppif pkrrtadpph vatlqmiqhh gefeedntes npsstgtntn
      961 deseriltdq dadrrkfder etiaeppvyv vypvntavni hpddsrekde svvvgtrgph
     1021 rplppetllq dneeqemnee sgpivqnmfn srpvasdfpy plerpdpsil vtgmretpll
     1081 vpsdqrqeds tkesneekde kdssvnvipy lqdfvpfpar knevisatly rgpsppsstp
     1141 iafvytptaq ashrldvdvr deednsksek nepkpvllps qqpssssssa pspqnfmapf
     1201 vasvsaeaps kngwsvvmve paanrksded rdslenpsdt eesqteksef dvenfkpqlf
     1261 ggfkpiyefp tqdedraerr esgddnseav tsvqqsvnst v
//
LOCUS       XP_034186644            1301 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607257 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186644
VERSION     XP_034186644.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330753.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186644.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1301
                     /product="uncharacterized protein LOC117607257 isoform X2"
                     /calculated_mol_wt=143836
     CDS             1..1301
                     /gene="LOC117607257"
                     /coded_by="XM_034330753.2:381..4286"
                     /db_xref="GeneID:117607257"
ORIGIN      
        1 mtrkmqpnpw tlmialafva svsseylmgg hmdngppksl leemessptl artaqlddld
       61 ldldaegstv alstsndeea pryhlpypfa fnggrpfsle kdpitgkidf ekappvkaln
      121 ytsryqvpvn deeqpddkef snedtkknae nketvdvspn einpyspnfh dflnlpvhys
      181 sdkygkdkyp lisssyantk vqsgsnsyst ynhkpyhget vayyhtrkpy vskttsattt
      241 ttnttprtts sstttttttt ttpkpvttst tttttttttt tppppsstas vstkapvvtt
      301 wkppsttkrf vgqlddyedi lpieklqatm ynnrhqgtnn imhnrdpqtk psmqheeymd
      361 syedyemndg ddyvpvkdst neivktstlp attstisnvt gttvgtpetp vsttavtsta
      421 tpsassthss svlqentyqt nsvpfqalpq rpvtsmpldn dprfpsgfen dnrkqgiadn
      481 vivnqnyrpn pivgqrpsgl sggyvvests nivippdqdt vsfvlgnrqn veggyysvgt
      541 aigenpyvgs pgidtsfrpl ygvpsdkgny dpqtdhlgti glpsvsvlen npnyapqawr
      601 qpnpsasqkl gneiepsrpq nsfvkgtvlm eqaddkkddk dvnfvvfpkd epkqpveehi
      661 vvineadgtv heitassttk kpvrvnppvl neehlpqlse dltppterpr pppqfhyhyh
      721 hggtarpdhp rpqrlpippr gkqppspafp pppppseavg irrrpyspdt nlpnilpqfr
      781 pnaktshghr gsesigtipa gqtypstrir qpvqshpptr rplppppsyl qrlnpppppi
      841 halrlaaaas tktenvippr epeptvkrfr pppipatsrg eddvklaqrl ssqklrleee
      901 erseryseep pqlpprppif pkrrtadpph vatlqmiqhh gefeedntes npsstgtntn
      961 deseriltdq dadrrkfder etiaeppvyv vypvntavni hpddsrekde svvvgtrgph
     1021 rplppetllq dneeqemnee sgpivqnmfn srpvasdfpy plerpdpsil vtgmretpll
     1081 vpsdqrqeds tkesneekde kdssvnvipy lqdfvpfpar knevisatly rgpsppsstp
     1141 iafvytptaq ashrldvdvr deednsksek nepkpvllps qqpssssssa pspqnfmapf
     1201 vasvsaeaps kngwsvvmve paanrksded rdslenpsdt eesqteksef dvenfkpqlf
     1261 ggfkpiyefp tqdedraerr esgddnseav tsvqqsvnst v
//
LOCUS       XP_034186651             960 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger protein swm [Osmia lignaria lignaria].
ACCESSION   XP_034186651
VERSION     XP_034186651.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330760.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..960
                     /product="zinc finger protein swm"
                     /calculated_mol_wt=107305
     Region          7..72
                     /region_name="PWI"
                     /note="PWI domain; pfam01480"
                     /db_xref="CDD:460224"
     Region          325..348
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          497..570
                     /region_name="RRM1_RBM26_like"
                     /note="RNA recognition motif 1 (RRM1) found in vertebrate
                     RNA-binding protein 26 (RBM26) and similar proteins;
                     cd12257"
                     /db_xref="CDD:409702"
     Region          698..>781
                     /region_name="OmpH"
                     /note="Outer membrane protein (OmpH-like); pfam03938"
                     /db_xref="CDD:461098"
     Region          824..895
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..960
                     /gene="swm"
                     /coded_by="XM_034330760.2:456..3338"
                     /db_xref="GeneID:117607259"
ORIGIN      
        1 miienpdqfk awltavlepl cdadpaalak yvyalvkkdk tleelrggmv eqldvflqqe
       61 tknfvellfk tletqeyvlp pakndpdggg tppgvnpppp alavekvtes tipiasvntn
      121 ppvplqmngs apmtigkret rksdsdkvdk ekekrsrsrs grmrsrtrsr srswerdrrr
      181 srsrehirrd rerdrsrpwr nesppntrrh drrrsrsrst spirprirdg pdnrdhrarf
      241 rnrsptplrs rsrsrsldrk kidrsermdv drtdriegsp gggtptqdsn hgdvdmrlst
      301 tsqsiqsvva vasnvpnsqp gfqtkrrcrd fdekgycmrg dlcpydhgtd pvvledvals
      361 rvltfgphsa qapgtvpvta vpepppgpng nappqhlpla slppphlrnq hhsnmdafae
      421 ynpdapsmep rmpwgrhpqp gpgiygrgqr elisvpviph tnssevthtq tnplkrkqaf
      481 dfnrlgpkqr vvhnpancsl elkkvprsln nitqlnnhfs kfgkivniqv nfggdpegal
      541 vtfqlpaeak aayrsteavl nnrfikvfwh nnvnnnaagg aienvppgcr psvkerlgaa
      601 vnvpaktden eyiptrrste eqitqttvpt spkttpvptr eekvlaikkt qeilaaketl
      661 kkkqeekrke alkltadlrk rkqelldkhl ieiralieka eknpeqkdai matiktmqqs
      721 idnlrkdlaa ngqiggnktq vksreqtqke ildaeldlmt aqqegqdage lqkrlnelra
      781 qaaalglntg tavgrgtrps rvirgthals yrgrgrgsft hvsvdhrpts llvsgyetee
      841 kaevlahfqq fgeivsqivd datpsiviny ksrkeaeval akgrtfqdrl lsitwvsghh
      901 lhrggsgsnt ntsvqlssrs eqappttded vdlegnaeal lleeneeeed edgesrswrr
//
LOCUS       XP_034186652             890 aa            linear   INV 03-SEP-2025
DEFINITION  S1 RNA-binding domain-containing protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186652
VERSION     XP_034186652.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330761.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186652.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..890
                     /product="S1 RNA-binding domain-containing protein 1"
                     /calculated_mol_wt=100163
     Region          <10..>220
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          156..887
                     /region_name="Tex"
                     /note="Transcriptional accessory protein Tex/SPT6
                     [Transcription]; COG2183"
                     /db_xref="CDD:441786"
     CDS             1..890
                     /gene="LOC117607260"
                     /coded_by="XM_034330761.2:130..2802"
                     /db_xref="GeneID:117607260"
ORIGIN      
        1 mirislfiys tvnsvkhrcl sitskqncim krslrkttqi kqiielsdse nddnvikddd
       61 yvpekkvskv dqtrkrirkd dpnvdrkkrk sstlllpske eqtdlrekiq skrtkskktv
      121 tqaidkenvk elknfqfnsv qckewteldy vcekngidkh taenviklfn dentipfiar
      181 yrknitggle pdklralkes ldhaklikqr aatitksidk lgqwspevha aivsakcldd
      241 lehiysffkp askaslaeka rklglgpvsd silqghplpq lsslisdkdg lkteeqikdg
      301 iihiiaetin kdkmvfdrvk elqsvsiisi qsteckssks kndkkegdkk rkyemyynfd
      361 tntklikphh ilainrgeaq kilsvklslp nsfeeqfkay clklyrqama asklhteilh
      421 nsidyaykkf ikplvtrrvr nelkqkaeta sievfasnvk qlllippvrg kvilgidpgf
      481 yhgcklavis ehgdvldtnv iyphgssdmf ekssdilidl vkkhkctila lgnatacret
      541 esfldkliks kafgsldvly tivdetgasi yscnaeakse fpdldtnvis aisiarrlqd
      601 plaelvkiep khlgvgmyqh dlpekqlltt lnevvseavs fvgvdvntas qcllkrvagl
      661 navrtkniiq wrtefgafkn rqqlldvkgi gnkvfeqcag firilpetsm tdnsvkikds
      721 kksihsfnll dqtwihpesy kiaerflkyc ncnlenlgtt tfiekiksha degyavlaek
      781 cgtnattmei ivkglsmkkd edirfktagp lfqknmitid dintetvltg avrnvthfgv
      841 fvdigvgtcg lipttllnns tvsigqrvev kvvakdpcrn ritlklinvl
//
LOCUS       XP_076548140             590 aa            linear   INV 03-SEP-2025
DEFINITION  wee1-like protein kinase [Osmia lignaria lignaria].
ACCESSION   XP_076548140
VERSION     XP_076548140.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692025.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..590
                     /product="wee1-like protein kinase"
                     /calculated_mol_wt=65851
     Region          233..507
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(240..243,246,248,261,263,295,311..314,361,365..366,
                     368,402..403)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..590
                     /gene="LOC117607262"
                     /coded_by="XM_076692025.1:603..2375"
                     /db_xref="GeneID:117607262"
ORIGIN      
        1 mevaqklhfd acdiqdeeln iscrtnssgc dvddildssa edlgcspplq prklsfsntm
       61 dcsdsennqs vlinnnktpi snvtgasrir ipprenvcsq kmsmacsppy krvralrlfd
      121 spatpktlme ksamhtpfpt kctrlfsldk pracsyqtks dkpaanvnpf tpngmlltar
      181 krtrskrsln gspdiqipkf dladseesdn eeveqatkrv alqdsnipry hqefhelgli
      241 gtgefgsvyk cinrldgciy aikksikpva gsineknaln evyahavlgk hqhvvryysa
      301 waednhmiiq neycnggsla daivnleken kkfteaeirq lllhvaeglr yihsmqlvhm
      361 dikpgnifis kekrllavny dsaddgfdee etieeeityk igdlghvtsv nnpqveegdc
      421 rylpteilre dfshlskadi falgltvyea ggggplpkng pewhdirngn lkelphysrd
      481 vnellklmvh pnpemrpsav cliqhrvlcp fgnktkaqlr relnaeklkn eilskqlqea
      541 akclktiapn vaainnnmna ggyklrptpt rtssrvvgkk vnrsnsttnf
//
LOCUS       XP_034186653             590 aa            linear   INV 03-SEP-2025
DEFINITION  wee1-like protein kinase [Osmia lignaria lignaria].
ACCESSION   XP_034186653
VERSION     XP_034186653.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330762.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..590
                     /product="wee1-like protein kinase"
                     /calculated_mol_wt=65851
     Region          233..507
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(240..243,246,248,261,263,295,311..314,361,365..366,
                     368,402..403)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..590
                     /gene="LOC117607262"
                     /coded_by="XM_034330762.2:532..2304"
                     /db_xref="GeneID:117607262"
ORIGIN      
        1 mevaqklhfd acdiqdeeln iscrtnssgc dvddildssa edlgcspplq prklsfsntm
       61 dcsdsennqs vlinnnktpi snvtgasrir ipprenvcsq kmsmacsppy krvralrlfd
      121 spatpktlme ksamhtpfpt kctrlfsldk pracsyqtks dkpaanvnpf tpngmlltar
      181 krtrskrsln gspdiqipkf dladseesdn eeveqatkrv alqdsnipry hqefhelgli
      241 gtgefgsvyk cinrldgciy aikksikpva gsineknaln evyahavlgk hqhvvryysa
      301 waednhmiiq neycnggsla daivnleken kkfteaeirq lllhvaeglr yihsmqlvhm
      361 dikpgnifis kekrllavny dsaddgfdee etieeeityk igdlghvtsv nnpqveegdc
      421 rylpteilre dfshlskadi falgltvyea ggggplpkng pewhdirngn lkelphysrd
      481 vnellklmvh pnpemrpsav cliqhrvlcp fgnktkaqlr relnaeklkn eilskqlqea
      541 akclktiapn vaainnnmna ggyklrptpt rtssrvvgkk vnrsnsttnf
//
LOCUS       XP_034186660             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186660
VERSION     XP_034186660.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330769.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330769.2:759..2480"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186661             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186661
VERSION     XP_034186661.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330770.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330770.2:2806..4527"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186665             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186665
VERSION     XP_034186665.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330774.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330774.2:684..2405"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_076548141             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_076548141
VERSION     XP_076548141.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692026.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_076692026.1:1030..2751"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_076548142             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_076548142
VERSION     XP_076548142.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692027.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_076692027.1:851..2572"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186667             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186667
VERSION     XP_034186667.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330776.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330776.2:663..2384"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186659             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186659
VERSION     XP_034186659.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330768.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330768.2:812..2533"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186664             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186664
VERSION     XP_034186664.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330773.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330773.2:967..2688"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186666             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186666
VERSION     XP_034186666.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330775.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330775.2:1649..3370"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186669             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186669
VERSION     XP_034186669.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330778.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330778.2:683..2404"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186670             573 aa            linear   INV 03-SEP-2025
DEFINITION  organic cation transporter [Osmia lignaria lignaria].
ACCESSION   XP_034186670
VERSION     XP_034186670.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330779.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..573
                     /product="organic cation transporter"
                     /calculated_mol_wt=64455
     Region          11..525
                     /region_name="2A0119"
                     /note="cation transport protein; TIGR00898"
                     /db_xref="CDD:273328"
     CDS             1..573
                     /gene="Orct"
                     /coded_by="XM_034330779.2:933..2654"
                     /db_xref="GeneID:117607263"
ORIGIN      
        1 mayddvilrm gefgryqrri ylmlclpais cafhkvagvf lgakmntrcv lpyenienat
       61 yclpqdimns sypwdnking wsqcyryevp givndtmfnr yldnilvqdd lnpqrimpce
      121 gyvydrrkyk stttsewnli cdkawmratg dalfmvgvml gsmifgglsd kfgrrpiffl
      181 slviqlvggi lvavspefis yvifrlivgs ttsgvflvay vialemvgpk krlvagvgcq
      241 lffttgyilt aafayfitdw rmlqvaitip siafllywwf ipesarwllt kgrtqeakdi
      301 lqraslengv empsevldtl lnnnsedtap dyrkpslfdl frypnlrrks illffnwlvn
      361 sgtyyglswh vsnldgndyv nfvisglvei paytfliftl nrwgrkiilc gcmlvsgval
      421 lgillvpada pwmvvclami gkltitssyg aiyvftaeqf ptvirnvglg asstfarigg
      481 viapyvihls eiwmplpfvi fgscvlsggm lslllpetln kklpesiqdg elfgkkiskk
      541 kkkhqldmdq pnsidvikpl kpqengvnek qng
//
LOCUS       XP_034186671             453 aa            linear   INV 03-SEP-2025
DEFINITION  excitatory amino acid transporter 3 [Osmia lignaria lignaria].
ACCESSION   XP_034186671
VERSION     XP_034186671.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330780.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="excitatory amino acid transporter 3"
                     /calculated_mol_wt=49813
     Region          8..435
                     /region_name="GltP"
                     /note="Na+/H+-dicarboxylate symporter [Energy production
                     and conversion]; COG1301"
                     /db_xref="CDD:440912"
     CDS             1..453
                     /gene="LOC117607264"
                     /coded_by="XM_034330780.2:235..1596"
                     /db_xref="GeneID:117607264"
ORIGIN      
        1 mkmlkkilrq klilrtvagv cagitiglvl kistpqpwsk rnimylkflg evfmrmvncl
       61 ilplvmssiv satcnlkksg rigtmalyyy ttttllgitl svvlvqtikp gellknenii
      121 sqnstksfmt vdtildlfrn lvpenimsac lsqyqtmlvk pknesvpiye wqidhttitg
      181 tnvlglvffs lilglaigei etkgeplinf frslsdammk imswaimlvp isalfliaak
      241 ilevedfesl ikrlgiyilt vftglliqgl iilpliyfic trqspyriiv klgpafvtaf
      301 gtssstatvp itisclerfg mpskicrfiv pigatinmdg ialyesigai fiiqlhglqf
      361 slfkviiisi tctvscigaa glpsggyvml imvlnsvgvp vedvsliiai dwfvdrfrtt
      421 lnivgdalga giithyynkn kqipgseeme lct
//
LOCUS       XP_034186672             380 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607265 [Osmia lignaria lignaria].
ACCESSION   XP_034186672
VERSION     XP_034186672.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330781.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..380
                     /product="uncharacterized protein LOC117607265"
                     /calculated_mol_wt=43137
     Region          46..370
                     /region_name="RNA_pol_I_A49"
                     /note="A49-like RNA polymerase I associated factor;
                     pfam06870"
                     /db_xref="CDD:462024"
     CDS             1..380
                     /gene="LOC117607265"
                     /coded_by="XM_034330781.2:74..1216"
                     /db_xref="GeneID:117607265"
ORIGIN      
        1 mkrpieaiie dviidstkvq piivnfqnge lkdeeakkms tglfydpkkq ktmvalsngq
       61 ivyrgykpdt sqdlmrtmlv lhnrktgkvr lvqverwqvn avlgkpivdn kksaddenia
      121 tlnkqfgskk akriteqyer mkinvdsvke qleqtvsnve vdradlsmql peneyitnta
      181 lphcnrdatn vtdvynafdi vpestlktly eraaeimntd vndfegkakf fvwslkrlks
      241 dpdnikkaal liyiqavvtw lnmpirdakk rhvdvcpsse qvnahvmdty siqsntgrlr
      301 pssvkdkavi hcmilaliic nfvldielfa tifthrmglk kltdlariig alpnkedkkl
      361 vtlkvplppp msvvnkkkrk
//
LOCUS       XP_034186686             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186686
VERSION     XP_034186686.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330795.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_034330795.2:1417..2418"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548143             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548143
VERSION     XP_076548143.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692028.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692028.1:1110..2111"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548144             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548144
VERSION     XP_076548144.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692029.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692029.1:1310..2311"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548145             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548145
VERSION     XP_076548145.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692030.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692030.1:972..1973"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548146             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548146
VERSION     XP_076548146.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692031.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692031.1:1003..2004"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548147             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548147
VERSION     XP_076548147.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692032.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692032.1:904..1905"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548148             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548148
VERSION     XP_076548148.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692033.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692033.1:408..1409"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548149             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548149
VERSION     XP_076548149.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692034.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692034.1:805..1806"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548150             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548150
VERSION     XP_076548150.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692035.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692035.1:813..1814"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548151             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548151
VERSION     XP_076548151.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692036.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692036.1:309..1310"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_076548152             333 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548152
VERSION     XP_076548152.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692037.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="sodium/bile acid cotransporter 7-B isoform X1"
                     /calculated_mol_wt=36971
     Region          24..329
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..333
                     /gene="LOC117607266"
                     /coded_by="XM_076692037.1:137..1138"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv liacvgqlfv sclcwilcsq wlprdivlat lftsthksvg lgswilrgay
      301 hgsahgpavn lplcilpvaq lllgsllasw lsp
//
LOCUS       XP_034186687             283 aa            linear   INV 03-SEP-2025
DEFINITION  sodium/bile acid cotransporter 7-B isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186687
VERSION     XP_034186687.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330796.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..283
                     /product="sodium/bile acid cotransporter 7-B isoform X2"
                     /calculated_mol_wt=31759
     Region          24..>174
                     /region_name="SBF"
                     /note="Sodium Bile acid symporter family; cl19217"
                     /db_xref="CDD:450274"
     CDS             1..283
                     /gene="LOC117607266"
                     /coded_by="XM_034330796.2:1417..2268"
                     /db_xref="GeneID:117607266"
ORIGIN      
        1 mnlnyiqenh qmkrhsklly rygyflsmlf cmilasirph fgktngilng nviiryfavp
       61 ltyleagllc dpkslystln ngplliftmi fiyilmpflm kigvyllvya dvniwllkgm
      121 evlycmpppf ntsfvlcrla kadlstsivi tlvshfgglf ispillyfvl gastpplvgv
      181 niketvysti ipliigitiq ivilkydfcf kvkspwfsqa lllatayhwf cdavlvdass
      241 lqatdvllcv lieslmfkla wdnsllaayv gfcalngylg isy
//
LOCUS       XP_034186689             318 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial potassium channel [Osmia lignaria lignaria].
ACCESSION   XP_034186689
VERSION     XP_034186689.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330798.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186689.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="mitochondrial potassium channel"
                     /calculated_mol_wt=36520
     CDS             1..318
                     /gene="LOC117607267"
                     /coded_by="XM_034330798.2:144..1100"
                     /db_xref="GeneID:117607267"
ORIGIN      
        1 maekfrslik lvtnrinthq lfnnattrin natgrvqekf nnvqnavaak ydvvskqink
       61 ditvmqnlna pqlepsllpk kvlkwwqwyq qltgldtvel akqqvitaqd klfkcqdnrr
      121 hlnrqatlin nklkevysel iqtkrddpky vqltiienks lqdqakivsq ldllekeekd
      181 hftllataik eyhdsqtmna qkykylsila savlaiisli gsmtynyski isirnviaea
      241 qqrnesvven nlntlennmn rlfsevikrl dnnnsneqki nvvtkelntn tlkemqttyi
      301 vlglcfvgly tiakmlig
//
LOCUS       XP_034186690             220 aa            linear   INV 03-SEP-2025
DEFINITION  suppressor of G2 allele of skp1 [Osmia lignaria lignaria].
ACCESSION   XP_034186690
VERSION     XP_034186690.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330799.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..220
                     /product="suppressor of G2 allele of skp1"
                     /calculated_mol_wt=24504
     Region          <18..218
                     /region_name="PLN03088"
                     /note="SGT1, suppressor of G2 allele of SKP1; Provisional"
                     /db_xref="CDD:215568"
     CDS             1..220
                     /gene="Sgt1"
                     /coded_by="XM_034330799.2:102..764"
                     /db_xref="GeneID:117607268"
ORIGIN      
        1 maneetaaqt ggnempipki khdwyqteth vivtilakns envkvvyekt tlsvsamlps
       61 gneytleldl ahpivpeqcs hkvvpskiei klkkqdgirw tvlegnpvvq npvqpiprei
      121 lqagnqppky pssskksrdw dkvekeiakq eaeekpegea alnalfqqiy gsssdevrra
      181 mnksfqesgg tvlstnwsev skgkverkpp dgmewkpwst
//
LOCUS       XP_076548153             201 aa            linear   INV 03-SEP-2025
DEFINITION  prenylated Rab acceptor protein 1-like [Osmia lignaria lignaria].
ACCESSION   XP_076548153
VERSION     XP_076548153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692038.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="prenylated Rab acceptor protein 1-like"
                     /calculated_mol_wt=22495
     Region          48..183
                     /region_name="PRA1"
                     /note="PRA1 family protein; pfam03208"
                     /db_xref="CDD:460846"
     CDS             1..201
                     /gene="LOC143306056"
                     /coded_by="XM_076692038.1:59..664"
                     /db_xref="GeneID:143306056"
ORIGIN      
        1 madveidvgg emqnpqsqqk eksgnglefl qiplnnlgyp qahewiehrk anvrpwsvfl
       61 ntsnikcppn lsrlnkriir nieyfqsnyl fvfiglviyc lvtspllllt vaaslgtcyk
      121 vsqrhakqel mflnklslaq iytliaiftl pvfilvgaga alfwvlgvsw flitlhaavy
      181 nidsvlcpge delnalvmqe v
//
LOCUS       XP_034186704            3168 aa            linear   INV 03-SEP-2025
DEFINITION  protocadherin-like wing polarity protein stan isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186704
VERSION     XP_034186704.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330813.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186704.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3168
                     /product="protocadherin-like wing polarity protein stan
                     isoform X1"
                     /calculated_mol_wt=348021
     Region          266..362
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(272..273,325,327,358,360..361)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          370..479
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(377..378,439,441,475,477..478)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          487..586
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(494..495,549,551,582,584..585)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          594..697
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(601..602,660,662,693,695..696)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          706..798
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(712..713,765,767,796,798)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          808..908
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(815..816,871,873,904,906..907)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          916..1011
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(923..924,975,977,1008,1010..1011)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1021..1119
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1027..1028,1084,1086,1115,1117..1118)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1138..1216
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1387..1422
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1470..1648
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          1674..1704
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          1714..1854
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          1914..1948
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          1939..2052
                     /region_name="TNFRSF"
                     /note="Tumor necrosis factor receptor superfamily
                     (TNFRSF); cl22855"
                     /db_xref="CDD:473981"
     Region          2007..2052
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(2007,2009,2019,2026,2028,2037)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2056..2118
                     /region_name="HormR"
                     /note="Domain present in hormone receptors; smart00008"
                     /db_xref="CDD:214468"
     Region          2136..2399
                     /region_name="GAIN"
                     /note="GPCR-Autoproteolysis INducing (GAIN) domain;
                     pfam16489"
                     /db_xref="CDD:465137"
     Region          2435..2487
                     /region_name="GPS"
                     /note="GPCR proteolysis site, GPS, motif; cl02559"
                     /db_xref="CDD:470616"
     Region          2497..2754
                     /region_name="7tmB2_CELSR_Adhesion_IV"
                     /note="cadherin EGF LAG seven-pass G-type receptors, group
                     IV adhesion GPCRs, member of the class B2 family of
                     seven-transmembrane G protein-coupled receptors; cd15441"
                     /db_xref="CDD:320557"
     Region          2499..2524
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320557"
     Site            order(2504,2508,2550,2553..2554,2557,2568,2575,2647..2648,
                     2650,2652,2704,2707,2719,2723)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:320557"
     Region          2533..2555
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2568..2595
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2607..2627
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2644..2673
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2685..2712
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2716..2741
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320557"
     CDS             1..3168
                     /gene="stan"
                     /coded_by="XM_034330813.2:502..10008"
                     /db_xref="GeneID:117607281"
ORIGIN      
        1 marwkwmlpl laafctltda ylavlssstp tgsvvfeagv pqlggrrkye vscertawfa
       61 rkllkvhpht grvtlarpls cdglqyprif tfyvdstssr lgrptidyys lplrilitgc
      121 ggenrdlaat rgwmaetlas yampsterft evclrtsqlv aalrdflpqt alkecetrwg
      181 gvadprflie gaagdlvsat eqclvdplwk isvsmnlrcg mesrltdaeh rlkivfhhrq
      241 lddtdlgrrv rrelknqspy feqplymgav eeekdpgvyv tsvrardpeg gtvrysmssl
      301 ldarsqsllv ldpvtgrvtt rarldresvd vhyfrvlavd dsfpprtgtt tlqvnvldan
      361 dhapsfewpe ydasvregvp vgstvvtvka tdqdasrnae leytilstts gsgtvntdda
      421 ltfridprsg vvttrtpldr ektevytvml svsdqatpps arktanatlv vrvlddndny
      481 pqftertysv wipedldyta npviarirat dadygnnaav ryaiiggntq ntfsidsmsg
      541 dvalvkpldy estrsykivi raqdggtpar snttqllvhv kdvndnaprf ytshfqesvs
      601 envaigysml riqaydadeg anaqikytig ardfsgaste nfpitvnset gwiyttkqld
      661 reqcsryqft viaadsgesp ksasatvilt vtdvndndpy fdpknyeavv seddppgtpv
      721 tsvtatdpde darihyeitt gntrgrfsia sqngrglitv aqpldykqek rfvltvtasd
      781 sggrtdtalv yvnvsdannf spvfenapys vsvfedapig ttvlvvsatd sdvgknaqvt
      841 ysldtdggdq avseftinpq tgaitttrpl drelvpayll tvtardggvp plsdttdvei
      901 svtdvndnsp vfeapqyqgs ipedvlvgts vlrvsatdad tdlngrvrya ldddgdgafa
      961 vdsttgivrt akpldresva ryvlkaiamd rgspslsttv pvtikiedin dsppafendk
     1021 ivlyiaensp igstvgeiya hdpdegpnav vqysvigged snsfalnvrp gsdraelitl
     1081 eeldyespkk kfelvvraas pplrsdalvq ilvtdvndna pvlkdfqiif nnfkdffpta
     1141 pigkipavda dvtdkltyti lagnnanlie lnrttgeirl spqlntnvpr vatmevsvtd
     1201 ginevkatmt lsvrlitdkm llnsitvrld dmtaeaflsp llgyfldgla aiipcpreni
     1261 flfsiqedan vhgkilnvsf sarkvepgvs defyspqflq ervylnrgil aklatvtvlp
     1321 fddnlcvrep clnfeecltv lkfgnasgfa ssdtvlfrpi ypvttfacrc akgftgskes
     1381 ylcdtevnlc ysnpcmndgt cyrreggyac scgpefagen ceisfekdsc epgickggsq
     1441 ctikttggft cegcpvatle nvtplcelka rsfgpetflt faslkqrhrl hiklrfates
     1501 anglllyngr ynekhdfval eiidsqvqfs fslgdeitra sasipggvsd gqwhevevsy
     1561 inktvtvsld ncdialaley geilgpkwsc agrseqvlee rcsiitetch rfldltgplq
     1621 lgglpaipss fqirnkdfvg cisdlyidhr fidlnsfvad ngtnpgcpek vsfcssipck
     1681 nngkcrevws gfvceceenf agptcadevg kpwnfhgdgm lsfnpllrpi qlpwltalsl
     1741 rtrskqaflm svqigqnssa lleirdgklv alldgsdiiq tsnniadgew hrieilwqsg
     1801 qvsldqdyrn rpissslpak lqglyvgril vggpeqstnt elpffdgclq dirigtnqsv
     1861 llrptvqenv atgcsseaec nadcpdasic makwqasecv caagrvgrnc ervcdlnpcn
     1921 ntstcledps srrgyrcecd speysgdyce ikadqpcpat wwgspvcgpc hcdeskgydp
     1981 acnkttgecy ckenhyqpsg kkecipcecy atgsfgprcd tetgqcrcrv gvigrsctac
     2041 pnpyaevtlr gcevvydgcp rsyaeglwwp rtkfgmtave dcpdtaegkt srscddklgg
     2101 wqepdlfnct seafieqrhq laaletkdlt lntyvavkia kdvhiavnlt kelygadlli
     2161 aeslliallr yeeslvglnl thsqdkdyvs hlvgiassil kskykekwsr ietlngdtpd
     2221 kildamanyl ktltasqhdt ftspfevvdt nvvlgldvvt seslfgyetv eykedpsast
     2281 srpseadqkv vipdtsgfls spglfgpsit fpkynnymad psrfdpyski rvpldilgik
     2341 slmqgelnvk kslvnrkavl syvqykqlga llpqrfddsv avrwgvevav gspvvtvsvl
     2401 vpsdegyesl tgiplqspvq islwlsekdd lktrtnpqcv hwsttrgvge wsrlgcttei
     2461 eddslsfgti vncscfqlst failtdvldl eyvpepslle dvtsysafml alplllsall
     2521 ilalirggst nsnsihknlv mcvffaevly lialkarspl vsnefpcklt aiglhyawls
     2581 tfswtlvdsi hlyrmltemr dvnhgqmrfy ytmgygapai ivgltigvra dqygnfyfcw
     2641 lsiyetviws ligpvcvavf vnfcilimsv raaftlkehi mgfgnlrtll wlsvaslpll
     2701 gstwtltvln asensptlsy llsiavvvha afsligycfi ngrvrrnlyl sllrcigkkt
     2761 pllegsmgng sssqnvnghs rsalayssty sgaesvcrrv hvgvstsstt srstnktgss
     2821 pyrsdthlrh tststsnyns drdpylssrs hrsalhsrqe vtetrtqrrd sesdsdgsqa
     2881 egggrsldla sshssdeedv asrshrnmgv stqqpvahsy lpnihsnpae hlnilcsnte
     2941 lfpnikpiya prwssqlpea ylasnvmdvr gsqwsggtms dnemasnkts spnplpypem
     3001 sspqksqpde nysegeeklh hlgekylfpy taeedhtvsp tpyilpmssr ilassmshhs
     3061 qnsnhenlsg serygslkrg qslhgsqhdn lsgserygsl krgkitptvl edspeyilpm
     3121 sgrilsssls hdlqhsnela alrqqqqqqq yeqtitetek dtnaetsv
//
LOCUS       XP_034186706            2887 aa            linear   INV 03-SEP-2025
DEFINITION  protocadherin-like wing polarity protein stan isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186706
VERSION     XP_034186706.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330815.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186706.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2887
                     /product="protocadherin-like wing polarity protein stan
                     isoform X2"
                     /calculated_mol_wt=317035
     Region          266..362
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(272..273,325,327,358,360..361)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          370..479
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(377..378,439,441,475,477..478)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          487..586
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(494..495,549,551,582,584..585)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          594..697
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(601..602,660,662,693,695..696)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          706..798
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(712..713,765,767,796,798)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          808..908
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(815..816,871,873,904,906..907)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          916..1011
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(923..924,975,977,1008,1010..1011)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1021..1119
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Site            order(1027..1028,1084,1086,1115,1117..1118)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:206637"
     Region          1138..1216
                     /region_name="Cadherin_repeat"
                     /note="Cadherin tandem repeat domain; cd11304"
                     /db_xref="CDD:206637"
     Region          1387..1422
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          1470..1648
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          1674..1704
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          1714..1854
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          1914..1948
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          2007..2052
                     /region_name="EGF_Lam"
                     /note="Laminin-type epidermal growth factor-like domai;
                     smart00180"
                     /db_xref="CDD:214543"
     Site            order(2007,2009,2019,2026,2028,2037)
                     /site_type="active"
                     /note="EGF-like motif [active]"
                     /db_xref="CDD:238012"
     Region          2056..2118
                     /region_name="HormR"
                     /note="Domain present in hormone receptors; smart00008"
                     /db_xref="CDD:214468"
     Region          2136..2399
                     /region_name="GAIN"
                     /note="GPCR-Autoproteolysis INducing (GAIN) domain;
                     pfam16489"
                     /db_xref="CDD:465137"
     Region          2435..2487
                     /region_name="GPS"
                     /note="GPCR proteolysis site, GPS, motif; cl02559"
                     /db_xref="CDD:470616"
     Region          2497..2754
                     /region_name="7tmB2_CELSR_Adhesion_IV"
                     /note="cadherin EGF LAG seven-pass G-type receptors, group
                     IV adhesion GPCRs, member of the class B2 family of
                     seven-transmembrane G protein-coupled receptors; cd15441"
                     /db_xref="CDD:320557"
     Region          2499..2524
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320557"
     Site            order(2504,2508,2550,2553..2554,2557,2568,2575,2647..2648,
                     2650,2652,2704,2707,2719,2723)
                     /site_type="other"
                     /note="putative polypeptide ligand binding pocket
                     [polypeptide binding]"
                     /db_xref="CDD:320557"
     Region          2533..2555
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2568..2595
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2607..2627
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2644..2673
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2685..2712
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320557"
     Region          2716..2741
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320557"
     CDS             1..2887
                     /gene="stan"
                     /coded_by="XM_034330815.2:1..8664"
                     /db_xref="GeneID:117607281"
ORIGIN      
        1 marwkwmlpl laafctltda ylavlssstp tgsvvfeagv pqlggrrkye vscertawfa
       61 rkllkvhpht grvtlarpls cdglqyprif tfyvdstssr lgrptidyys lplrilitgc
      121 ggenrdlaat rgwmaetlas yampsterft evclrtsqlv aalrdflpqt alkecetrwg
      181 gvadprflie gaagdlvsat eqclvdplwk isvsmnlrcg mesrltdaeh rlkivfhhrq
      241 lddtdlgrrv rrelknqspy feqplymgav eeekdpgvyv tsvrardpeg gtvrysmssl
      301 ldarsqsllv ldpvtgrvtt rarldresvd vhyfrvlavd dsfpprtgtt tlqvnvldan
      361 dhapsfewpe ydasvregvp vgstvvtvka tdqdasrnae leytilstts gsgtvntdda
      421 ltfridprsg vvttrtpldr ektevytvml svsdqatpps arktanatlv vrvlddndny
      481 pqftertysv wipedldyta npviarirat dadygnnaav ryaiiggntq ntfsidsmsg
      541 dvalvkpldy estrsykivi raqdggtpar snttqllvhv kdvndnaprf ytshfqesvs
      601 envaigysml riqaydadeg anaqikytig ardfsgaste nfpitvnset gwiyttkqld
      661 reqcsryqft viaadsgesp ksasatvilt vtdvndndpy fdpknyeavv seddppgtpv
      721 tsvtatdpde darihyeitt gntrgrfsia sqngrglitv aqpldykqek rfvltvtasd
      781 sggrtdtalv yvnvsdannf spvfenapys vsvfedapig ttvlvvsatd sdvgknaqvt
      841 ysldtdggdq avseftinpq tgaitttrpl drelvpayll tvtardggvp plsdttdvei
      901 svtdvndnsp vfeapqyqgs ipedvlvgts vlrvsatdad tdlngrvrya ldddgdgafa
      961 vdsttgivrt akpldresva ryvlkaiamd rgspslsttv pvtikiedin dsppafendk
     1021 ivlyiaensp igstvgeiya hdpdegpnav vqysvigged snsfalnvrp gsdraelitl
     1081 eeldyespkk kfelvvraas pplrsdalvq ilvtdvndna pvlkdfqiif nnfkdffpta
     1141 pigkipavda dvtdkltyti lagnnanlie lnrttgeirl spqlntnvpr vatmevsvtd
     1201 ginevkatmt lsvrlitdkm llnsitvrld dmtaeaflsp llgyfldgla aiipcpreni
     1261 flfsiqedan vhgkilnvsf sarkvepgvs defyspqflq ervylnrgil aklatvtvlp
     1321 fddnlcvrep clnfeecltv lkfgnasgfa ssdtvlfrpi ypvttfacrc akgftgskes
     1381 ylcdtevnlc ysnpcmndgt cyrreggyac scgpefagen ceisfekdsc epgickggsq
     1441 ctikttggft cegcpvatle nvtplcelka rsfgpetflt faslkqrhrl hiklrfates
     1501 anglllyngr ynekhdfval eiidsqvqfs fslgdeitra sasipggvsd gqwhevevsy
     1561 inktvtvsld ncdialaley geilgpkwsc agrseqvlee rcsiitetch rfldltgplq
     1621 lgglpaipss fqirnkdfvg cisdlyidhr fidlnsfvad ngtnpgcpek vsfcssipck
     1681 nngkcrevws gfvceceenf agptcadevg kpwnfhgdgm lsfnpllrpi qlpwltalsl
     1741 rtrskqaflm svqigqnssa lleirdgklv alldgsdiiq tsnniadgew hrieilwqsg
     1801 qvsldqdyrn rpissslpak lqglyvgril vggpeqstnt elpffdgclq dirigtnqsv
     1861 llrptvqenv atgcsseaec nadcpdasic makwqasecv caagrvgrnc ervcdlnpcn
     1921 ntstcledps srrgyrcecd speysgdyce ikadqpcpat wwgspvcgpc hcdeskgydp
     1981 acnkttgecy ckenhyqpsg kkecipcecy atgsfgprcd tetgqcrcrv gvigrsctac
     2041 pnpyaevtlr gcevvydgcp rsyaeglwwp rtkfgmtave dcpdtaegkt srscddklgg
     2101 wqepdlfnct seafieqrhq laaletkdlt lntyvavkia kdvhiavnlt kelygadlli
     2161 aeslliallr yeeslvglnl thsqdkdyvs hlvgiassil kskykekwsr ietlngdtpd
     2221 kildamanyl ktltasqhdt ftspfevvdt nvvlgldvvt seslfgyetv eykedpsast
     2281 srpseadqkv vipdtsgfls spglfgpsit fpkynnymad psrfdpyski rvpldilgik
     2341 slmqgelnvk kslvnrkavl syvqykqlga llpqrfddsv avrwgvevav gspvvtvsvl
     2401 vpsdegyesl tgiplqspvq islwlsekdd lktrtnpqcv hwsttrgvge wsrlgcttei
     2461 eddslsfgti vncscfqlst failtdvldl eyvpepslle dvtsysafml alplllsall
     2521 ilalirggst nsnsihknlv mcvffaevly lialkarspl vsnefpcklt aiglhyawls
     2581 tfswtlvdsi hlyrmltemr dvnhgqmrfy ytmgygapai ivgltigvra dqygnfyfcw
     2641 lsiyetviws ligpvcvavf vnfcilimsv raaftlkehi mgfgnlrtll wlsvaslpll
     2701 gstwtltvln asensptlsy llsiavvvha afsligycfi ngrvrrnlyl sllrcigkkt
     2761 pllegsmgng sssqnvnghs rsalayssty sgaesvcrrv hvgvstsstt srstnktgss
     2821 pyrsdthlrh tststsnyns drdpylssrs hrsalhsrqv tyrsnrdtdt kkgfrvrfgr
     2881 vpgggrw
//
LOCUS       XP_034186707            1918 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis spg isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186707
VERSION     XP_034186707.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330816.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1918
                     /product="dedicator of cytokinesis spg isoform X1"
                     /calculated_mol_wt=219055
     Region          10..65
                     /region_name="SH3_DOCK_AB"
                     /note="Src Homology 3 domain of Class A and B Dedicator of
                     Cytokinesis proteins; cd11872"
                     /db_xref="CDD:212805"
     Site            order(12..16,23,28..30,32,36..37,41..42,44,52,54,56,58,
                     60..61,63,65)
                     /site_type="other"
                     /note="ELMO interaction site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Site            order(15,17,20,24,41..42,58,60..61)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Region          73..411
                     /region_name="DOCK_N"
                     /note="DOCK N-terminus; pfam16172"
                     /db_xref="CDD:465040"
     Region          419..603
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1220..1612
                     /region_name="DHR2_DOCK"
                     /note="Dock Homology Region 2, a GEF domain, of Dedicator
                     of Cytokinesis proteins; cl06123"
                     /db_xref="CDD:471388"
     Site            order(1300,1311,1313..1314,1316..1317,1320..1321,
                     1323..1324,1327..1328)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1339,1341,1365,1368..1371,1373..1374,1387..1389,
                     1406..1408,1443..1446,1457..1458,1461,1463,1500..1501,
                     1504,1524..1525,1527..1528,1531..1533,1536..1538,
                     1541..1542,1545,1575,1596..1597,1600)
                     /site_type="other"
                     /note="Rac/Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1536..1538,1541)
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212566"
     CDS             1..1918
                     /gene="spg"
                     /coded_by="XM_034330816.2:436..6192"
                     /db_xref="GeneID:117607282"
ORIGIN      
        1 mwtttkttky gvavynwrgd tryglpleig etvqileeca gwyrgfstkn ravkgifpss
       61 yvhlkpckie neglyesvip ledpvvrevt lvlrewdgiw krlyverety kfvtlrkvml
      121 ellewrrqll ggtlttdqtr elklriinkv dwgnrklgld lvprqgahmv dpdtmsvvel
      181 yhvlwfrskf qhvqsaensq gasargtlrr kehkkvlthh lyfcmrdfgh sigedteiyf
      241 slydakrney lserflvris kegfssfiek ihsnctiftd lgnadlsrdl ymvahvmrcg
      301 rmlysdsgkn kagtatyrrp hgvavlslae atqdhteele mtfkvcqgee kefhqlheqi
      361 irnnkcsplp gqpnygivvs lrvlhgelsq vreenpllfk nicltkklgf sdvimpgdvr
      421 ndlylklerg eferggkstg knievtilvl dadgkplegc lfgaagteds seyqsliiyh
      481 hnspswaetv rlaipidkfy gshvrfefrh cstrektdkk lfafafvrlm epggatlqdg
      541 lhelyiykce drskldslsy ltlptsarep natgtpafsr spkeavfvht llcstkltqn
      601 vdllsllqwk ahperiseal grvlrldgee lvkflqdild alfsmfhted gnstahsglv
      661 fqvlvsifsl ledskfehfk pvmdayisgh faaalvykgl lssvqhcadw vtaaekqepi
      721 ikcfrsleyi fkfiiqsrll faratagqye dsfkrdlycv faalnkmlgi pyemvlnsqm
      781 allysispvf eqlvavlpvl evakltctml dsvprepplp ltqakltaik nlttsslfre
      841 ddesrnlllv tmcrhlrihl irreelrsct eifgeilsfl hkrgrdtnkv nnciqhdvet
      901 lclsildvli qtilivints gpvlgclvac ligllqllde yhysrlweel ahagerkplk
      961 dfllrvylvl rdlvrqevfp pdwlvirmqa nnvilkslqe laqplafrfs geyfdsqlws
     1021 tyfnlavayl tqpslqleqf sevkrekive kygdmrvfmg fqilsmwtql aerklefipg
     1081 mvgpflevtl vpeselrkat lhiffdmmec eqrargsfkt veselidkld ilisenkgdd
     1141 eyrqlfntme hlsavlldrv qsedptwkes gtafitsitr llerlldyrs viqgdenrdk
     1201 rmsctvnlln fyknefnrke mylryiyklh dlhlaaenyt eagftmklya dqlgwgstil
     1261 ppdhshpqqp ewqrkevlyh kiihyldrgk cwekgiplck elavlyetrl ydyaklshvl
     1321 klqakfldni ltqlrpepey frvgfyglsf plfvrnklfi yrgleyerig aftqrlqtef
     1381 psaqilmkns ppdesiltse gqyiqicnvk pipeesslac rgaevpervv afylvndvrk
     1441 fifdrplhrg pvdrenefks lwierttltt eaklpgilrw feviekrsel lapvqyacet
     1501 mqsverelrr lvaqytaeph rninpfsmrl qgiidanvmg gitkyqeafl tpefarqnpd
     1561 mvphvnrlkg lildqmsvle aglnlhgqia pagvqplhkr lnerftqlkq glgplarqrt
     1621 ihhdsivnsp lpplptnekq rpatletags ryshadsdgl pedegfytkv dggpppipqr
     1681 evrprsvgyg ttpprpthqr slskplspkl plrhslptpt dgvdqtglrt swsepgpepa
     1741 pplpprgctp dkrdsntsti vppappkrla ykrntewstd ddseaqnepn dlrdsgista
     1801 slldfqshlt nlnnlgyedf eprsrcndim nisppsvina lnvstsnfas gmfqgshslp
     1861 gqevspppip pkahqdtpsa pstlervsnr tqlhghseny svpklqtlsm asdtestv
//
LOCUS       XP_034186708            1912 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis spg isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186708
VERSION     XP_034186708.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330817.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1912
                     /product="dedicator of cytokinesis spg isoform X2"
                     /calculated_mol_wt=218400
     Region          10..65
                     /region_name="SH3_DOCK_AB"
                     /note="Src Homology 3 domain of Class A and B Dedicator of
                     Cytokinesis proteins; cd11872"
                     /db_xref="CDD:212805"
     Site            order(12..16,23,28..30,32,36..37,41..42,44,52,54,56,58,
                     60..61,63,65)
                     /site_type="other"
                     /note="ELMO interaction site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Site            order(15,17,20,24,41..42,58,60..61)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Region          73..411
                     /region_name="DOCK_N"
                     /note="DOCK N-terminus; pfam16172"
                     /db_xref="CDD:465040"
     Region          419..603
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1214..1606
                     /region_name="DHR2_DOCK"
                     /note="Dock Homology Region 2, a GEF domain, of Dedicator
                     of Cytokinesis proteins; cl06123"
                     /db_xref="CDD:471388"
     Site            order(1294,1305,1307..1308,1310..1311,1314..1315,
                     1317..1318,1321..1322)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1333,1335,1359,1362..1365,1367..1368,1381..1383,
                     1400..1402,1437..1440,1451..1452,1455,1457,1494..1495,
                     1498,1518..1519,1521..1522,1525..1527,1530..1532,
                     1535..1536,1539,1569,1590..1591,1594)
                     /site_type="other"
                     /note="Rac/Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1530..1532,1535)
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212566"
     CDS             1..1912
                     /gene="spg"
                     /coded_by="XM_034330817.2:436..6174"
                     /db_xref="GeneID:117607282"
ORIGIN      
        1 mwtttkttky gvavynwrgd tryglpleig etvqileeca gwyrgfstkn ravkgifpss
       61 yvhlkpckie neglyesvip ledpvvrevt lvlrewdgiw krlyverety kfvtlrkvml
      121 ellewrrqll ggtlttdqtr elklriinkv dwgnrklgld lvprqgahmv dpdtmsvvel
      181 yhvlwfrskf qhvqsaensq gasargtlrr kehkkvlthh lyfcmrdfgh sigedteiyf
      241 slydakrney lserflvris kegfssfiek ihsnctiftd lgnadlsrdl ymvahvmrcg
      301 rmlysdsgkn kagtatyrrp hgvavlslae atqdhteele mtfkvcqgee kefhqlheqi
      361 irnnkcsplp gqpnygivvs lrvlhgelsq vreenpllfk nicltkklgf sdvimpgdvr
      421 ndlylklerg eferggkstg knievtilvl dadgkplegc lfgaagteds seyqsliiyh
      481 hnspswaetv rlaipidkfy gshvrfefrh cstrektdkk lfafafvrlm epggatlqdg
      541 lhelyiykce drskldslsy ltlptsarep natgtpafsr spkeavfvht llcstkltqn
      601 vdllsllqwk ahperiseal grvlrldgee lvkflqdild alfsmfhted gnstahsglv
      661 fqvlvsifsl ledskfehfk pvmdayisgh faaalvykgl lssvqhcadw vtaaekqepi
      721 ikcfrsleyi fkfiiqsrll faratagqye dsfkrdlycv faalnkmlgi pyemvlnsqm
      781 allysispvf eqlvavlpvl evakltctml dsvprepplp ltqakltaik nlttsslfre
      841 ddesrnlllv tmcrhlrihl irreelrsct eifgeilsfl hkrgrdtnkv nnciqhdvet
      901 lclsildvli qtilivints gpvlgclvac ligllqllde yhysrlweel ahagerkplk
      961 dfllrvylvl rdlvrqevfp pdwlvirmqa nnvilkslqe laqplafrfs geyfdsqlws
     1021 tyfnlavayl tqpslqleqf sevkrekive kygdmrvfmg fqilsmwtql aerklefipg
     1081 mvgpflevtl vpeselrkat lhiffdmmec eqrargsfkt veselidkld ilisenkgdd
     1141 eyrqlfntil ldrvqsedpt wkesgtafit sitrllerll dyrsviqgde nrdkrmsctv
     1201 nllnfyknef nrkemylryi yklhdlhlaa enyteagftm klyadqlgwg stilppdhsh
     1261 pqqpewqrke vlyhkiihyl drgkcwekgi plckelavly etrlydyakl shvlklqakf
     1321 ldniltqlrp epeyfrvgfy glsfplfvrn klfiyrgley erigaftqrl qtefpsaqil
     1381 mknsppdesi ltsegqyiqi cnvkpipees slacrgaevp ervvafylvn dvrkfifdrp
     1441 lhrgpvdren efkslwiert tltteaklpg ilrwfeviek rsellapvqy acetmqsver
     1501 elrrlvaqyt aephrninpf smrlqgiida nvmggitkyq eafltpefar qnpdmvphvn
     1561 rlkglildqm svleaglnlh gqiapagvqp lhkrlnerft qlkqglgpla rqrtihhdsi
     1621 vnsplpplpt nekqrpatle tagsryshad sdglpedegf ytkvdggppp ipqrevrprs
     1681 vgygttpprp thqrslskpl spklplrhsl ptptdgvdqt glrtswsepg pepapplppr
     1741 gctpdkrdsn tstivppapp krlaykrnte wstdddseaq nepndlrdsg istaslldfq
     1801 shltnlnnlg yedfeprsrc ndimnispps vinalnvsts nfasgmfqgs hslpgqevsp
     1861 ppippkahqd tpsapstler vsnrtqlhgh senysvpklq tlsmasdtes tv
//
LOCUS       XP_034186709            1910 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis spg isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186709
VERSION     XP_034186709.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330818.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1910
                     /product="dedicator of cytokinesis spg isoform X3"
                     /calculated_mol_wt=217962
     Region          10..65
                     /region_name="SH3_DOCK_AB"
                     /note="Src Homology 3 domain of Class A and B Dedicator of
                     Cytokinesis proteins; cd11872"
                     /db_xref="CDD:212805"
     Site            order(12..16,23,28..30,32,36..37,41..42,44,52,54,56,58,
                     60..61,63,65)
                     /site_type="other"
                     /note="ELMO interaction site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Site            order(15,17,20,24,41..42,58,60..61)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Region          73..403
                     /region_name="DOCK_N"
                     /note="DOCK N-terminus; pfam16172"
                     /db_xref="CDD:465040"
     Region          411..595
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1212..1604
                     /region_name="DHR2_DOCK"
                     /note="Dock Homology Region 2, a GEF domain, of Dedicator
                     of Cytokinesis proteins; cl06123"
                     /db_xref="CDD:471388"
     Site            order(1292,1303,1305..1306,1308..1309,1312..1313,
                     1315..1316,1319..1320)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1331,1333,1357,1360..1363,1365..1366,1379..1381,
                     1398..1400,1435..1438,1449..1450,1453,1455,1492..1493,
                     1496,1516..1517,1519..1520,1523..1525,1528..1530,
                     1533..1534,1537,1567,1588..1589,1592)
                     /site_type="other"
                     /note="Rac/Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1528..1530,1533)
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212566"
     CDS             1..1910
                     /gene="spg"
                     /coded_by="XM_034330818.2:436..6168"
                     /db_xref="GeneID:117607282"
ORIGIN      
        1 mwtttkttky gvavynwrgd tryglpleig etvqileeca gwyrgfstkn ravkgifpss
       61 yvhlkpckie neglyesvip ledpvvrevt lvlrewdgiw krlyverety kfvtlrkvml
      121 ellewrrqll ggtlttdqtr elklriinkv dwgnrklgld lvprqgahmv dpdtmsvvel
      181 yhvhvqsaen sqgasargtl rrkehkkvlt hhlyfcmrdf ghsigedtei yfslydakrn
      241 eylserflvr iskegfssfi ekihsnctif tdlgnadlsr dlymvahvmr cgrmlysdsg
      301 knkagtatyr rphgvavlsl aeatqdhtee lemtfkvcqg eekefhqlhe qiirnnkcsp
      361 lpgqpnygiv vslrvlhgel sqvreenpll fknicltkkl gfsdvimpgd vrndlylkle
      421 rgeferggks tgknievtil vldadgkple gclfgaagte dsseyqslii yhhnspswae
      481 tvrlaipidk fygshvrfef rhcstrektd kklfafafvr lmepggatlq dglhelyiyk
      541 cedrskldsl syltlptsar epnatgtpaf srspkeavfv htllcstklt qnvdllsllq
      601 wkahperise algrvlrldg eelvkflqdi ldalfsmfht edgnstahsg lvfqvlvsif
      661 slledskfeh fkpvmdayis ghfaaalvyk gllssvqhca dwvtaaekqe piikcfrsle
      721 yifkfiiqsr llfaratagq yedsfkrdly cvfaalnkml gipyemvlns qmallysisp
      781 vfeqlvavlp vlevakltct mldsvprepp lpltqaklta iknlttsslf reddesrnll
      841 lvtmcrhlri hlirreelrs cteifgeils flhkrgrdtn kvnnciqhdv etlclsildv
      901 liqtilivin tsgpvlgclv acligllqll deyhysrlwe elahagerkp lkdfllrvyl
      961 vlrdlvrqev fppdwlvirm qannvilksl qelaqplafr fsgeyfdsql wstyfnlava
     1021 yltqpslqle qfsevkreki vekygdmrvf mgfqilsmwt qlaerklefi pgmvgpflev
     1081 tlvpeselrk atlhiffdmm eceqrargsf ktveselidk ldilisenkg ddeyrqlfnt
     1141 mehlsavlld rvqsedptwk esgtafitsi trllerlldy rsviqgdenr dkrmsctvnl
     1201 lnfyknefnr kemylryiyk lhdlhlaaen yteagftmkl yadqlgwgst ilppdhshpq
     1261 qpewqrkevl yhkiihyldr gkcwekgipl ckelavlyet rlydyaklsh vlklqakfld
     1321 niltqlrpep eyfrvgfygl sfplfvrnkl fiyrgleyer igaftqrlqt efpsaqilmk
     1381 nsppdesilt segqyiqicn vkpipeessl acrgaevper vvafylvndv rkfifdrplh
     1441 rgpvdrenef kslwierttl tteaklpgil rwfeviekrs ellapvqyac etmqsverel
     1501 rrlvaqytae phrninpfsm rlqgiidanv mggitkyqea fltpefarqn pdmvphvnrl
     1561 kglildqmsv leaglnlhgq iapagvqplh krlnerftql kqglgplarq rtihhdsivn
     1621 splpplptne kqrpatleta gsryshadsd glpedegfyt kvdggpppip qrevrprsvg
     1681 ygttpprpth qrslskplsp klplrhslpt ptdgvdqtgl rtswsepgpe papplpprgc
     1741 tpdkrdsnts tivppappkr laykrntews tdddseaqne pndlrdsgis taslldfqsh
     1801 ltnlnnlgye dfeprsrcnd imnisppsvi nalnvstsnf asgmfqgshs lpgqevsppp
     1861 ippkahqdtp sapstlervs nrtqlhghse nysvpklqtl smasdtestv
//
LOCUS       XP_034186710            1904 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis spg isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034186710
VERSION     XP_034186710.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330819.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1904
                     /product="dedicator of cytokinesis spg isoform X4"
                     /calculated_mol_wt=217307
     Region          10..65
                     /region_name="SH3_DOCK_AB"
                     /note="Src Homology 3 domain of Class A and B Dedicator of
                     Cytokinesis proteins; cd11872"
                     /db_xref="CDD:212805"
     Site            order(12..16,23,28..30,32,36..37,41..42,44,52,54,56,58,
                     60..61,63,65)
                     /site_type="other"
                     /note="ELMO interaction site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Site            order(15,17,20,24,41..42,58,60..61)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Region          73..403
                     /region_name="DOCK_N"
                     /note="DOCK N-terminus; pfam16172"
                     /db_xref="CDD:465040"
     Region          411..595
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1206..1598
                     /region_name="DHR2_DOCK"
                     /note="Dock Homology Region 2, a GEF domain, of Dedicator
                     of Cytokinesis proteins; cl06123"
                     /db_xref="CDD:471388"
     Site            order(1286,1297,1299..1300,1302..1303,1306..1307,
                     1309..1310,1313..1314)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1325,1327,1351,1354..1357,1359..1360,1373..1375,
                     1392..1394,1429..1432,1443..1444,1447,1449,1486..1487,
                     1490,1510..1511,1513..1514,1517..1519,1522..1524,
                     1527..1528,1531,1561,1582..1583,1586)
                     /site_type="other"
                     /note="Rac/Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1522..1524,1527)
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212566"
     CDS             1..1904
                     /gene="spg"
                     /coded_by="XM_034330819.2:436..6150"
                     /db_xref="GeneID:117607282"
ORIGIN      
        1 mwtttkttky gvavynwrgd tryglpleig etvqileeca gwyrgfstkn ravkgifpss
       61 yvhlkpckie neglyesvip ledpvvrevt lvlrewdgiw krlyverety kfvtlrkvml
      121 ellewrrqll ggtlttdqtr elklriinkv dwgnrklgld lvprqgahmv dpdtmsvvel
      181 yhvhvqsaen sqgasargtl rrkehkkvlt hhlyfcmrdf ghsigedtei yfslydakrn
      241 eylserflvr iskegfssfi ekihsnctif tdlgnadlsr dlymvahvmr cgrmlysdsg
      301 knkagtatyr rphgvavlsl aeatqdhtee lemtfkvcqg eekefhqlhe qiirnnkcsp
      361 lpgqpnygiv vslrvlhgel sqvreenpll fknicltkkl gfsdvimpgd vrndlylkle
      421 rgeferggks tgknievtil vldadgkple gclfgaagte dsseyqslii yhhnspswae
      481 tvrlaipidk fygshvrfef rhcstrektd kklfafafvr lmepggatlq dglhelyiyk
      541 cedrskldsl syltlptsar epnatgtpaf srspkeavfv htllcstklt qnvdllsllq
      601 wkahperise algrvlrldg eelvkflqdi ldalfsmfht edgnstahsg lvfqvlvsif
      661 slledskfeh fkpvmdayis ghfaaalvyk gllssvqhca dwvtaaekqe piikcfrsle
      721 yifkfiiqsr llfaratagq yedsfkrdly cvfaalnkml gipyemvlns qmallysisp
      781 vfeqlvavlp vlevakltct mldsvprepp lpltqaklta iknlttsslf reddesrnll
      841 lvtmcrhlri hlirreelrs cteifgeils flhkrgrdtn kvnnciqhdv etlclsildv
      901 liqtilivin tsgpvlgclv acligllqll deyhysrlwe elahagerkp lkdfllrvyl
      961 vlrdlvrqev fppdwlvirm qannvilksl qelaqplafr fsgeyfdsql wstyfnlava
     1021 yltqpslqle qfsevkreki vekygdmrvf mgfqilsmwt qlaerklefi pgmvgpflev
     1081 tlvpeselrk atlhiffdmm eceqrargsf ktveselidk ldilisenkg ddeyrqlfnt
     1141 illdrvqsed ptwkesgtaf itsitrller lldyrsviqg denrdkrmsc tvnllnfykn
     1201 efnrkemylr yiyklhdlhl aaenyteagf tmklyadqlg wgstilppdh shpqqpewqr
     1261 kevlyhkiih yldrgkcwek giplckelav lyetrlydya klshvlklqa kfldniltql
     1321 rpepeyfrvg fyglsfplfv rnklfiyrgl eyerigaftq rlqtefpsaq ilmknsppde
     1381 siltsegqyi qicnvkpipe esslacrgae vpervvafyl vndvrkfifd rplhrgpvdr
     1441 enefkslwie rttltteakl pgilrwfevi ekrsellapv qyacetmqsv erelrrlvaq
     1501 ytaephrnin pfsmrlqgii danvmggitk yqeafltpef arqnpdmvph vnrlkglild
     1561 qmsvleagln lhgqiapagv qplhkrlner ftqlkqglgp larqrtihhd sivnsplppl
     1621 ptnekqrpat letagsrysh adsdglpede gfytkvdggp ppipqrevrp rsvgygttpp
     1681 rpthqrslsk plspklplrh slptptdgvd qtglrtswse pgpepapplp prgctpdkrd
     1741 sntstivppa ppkrlaykrn tewstdddse aqnepndlrd sgistaslld fqshltnlnn
     1801 lgyedfeprs rcndimnisp psvinalnvs tsnfasgmfq gshslpgqev spppippkah
     1861 qdtpsapstl ervsnrtqlh ghsenysvpk lqtlsmasdt estv
//
LOCUS       XP_034186712            1898 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis spg isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034186712
VERSION     XP_034186712.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330821.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1898
                     /product="dedicator of cytokinesis spg isoform X5"
                     /calculated_mol_wt=216915
     Region          10..65
                     /region_name="SH3_DOCK_AB"
                     /note="Src Homology 3 domain of Class A and B Dedicator of
                     Cytokinesis proteins; cd11872"
                     /db_xref="CDD:212805"
     Site            order(12..16,23,28..30,32,36..37,41..42,44,52,54,56,58,
                     60..61,63,65)
                     /site_type="other"
                     /note="ELMO interaction site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Site            order(15,17,20,24,41..42,58,60..61)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212805"
     Region          73..411
                     /region_name="DOCK_N"
                     /note="DOCK N-terminus; pfam16172"
                     /db_xref="CDD:465040"
     Region          419..603
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1220..1612
                     /region_name="DHR2_DOCK"
                     /note="Dock Homology Region 2, a GEF domain, of Dedicator
                     of Cytokinesis proteins; cl06123"
                     /db_xref="CDD:471388"
     Site            order(1300,1311,1313..1314,1316..1317,1320..1321,
                     1323..1324,1327..1328)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1339,1341,1365,1368..1371,1373..1374,1387..1389,
                     1406..1408,1443..1446,1457..1458,1461,1463,1500..1501,
                     1504,1524..1525,1527..1528,1531..1533,1536..1538,
                     1541..1542,1545,1575,1596..1597,1600)
                     /site_type="other"
                     /note="Rac/Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1536..1538,1541)
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212566"
     Region          1629..>1874
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..1898
                     /gene="spg"
                     /coded_by="XM_034330821.2:436..6132"
                     /db_xref="GeneID:117607282"
ORIGIN      
        1 mwtttkttky gvavynwrgd tryglpleig etvqileeca gwyrgfstkn ravkgifpss
       61 yvhlkpckie neglyesvip ledpvvrevt lvlrewdgiw krlyverety kfvtlrkvml
      121 ellewrrqll ggtlttdqtr elklriinkv dwgnrklgld lvprqgahmv dpdtmsvvel
      181 yhvlwfrskf qhvqsaensq gasargtlrr kehkkvlthh lyfcmrdfgh sigedteiyf
      241 slydakrney lserflvris kegfssfiek ihsnctiftd lgnadlsrdl ymvahvmrcg
      301 rmlysdsgkn kagtatyrrp hgvavlslae atqdhteele mtfkvcqgee kefhqlheqi
      361 irnnkcsplp gqpnygivvs lrvlhgelsq vreenpllfk nicltkklgf sdvimpgdvr
      421 ndlylklerg eferggkstg knievtilvl dadgkplegc lfgaagteds seyqsliiyh
      481 hnspswaetv rlaipidkfy gshvrfefrh cstrektdkk lfafafvrlm epggatlqdg
      541 lhelyiykce drskldslsy ltlptsarep natgtpafsr spkeavfvht llcstkltqn
      601 vdllsllqwk ahperiseal grvlrldgee lvkflqdild alfsmfhted gnstahsglv
      661 fqvlvsifsl ledskfehfk pvmdayisgh faaalvykgl lssvqhcadw vtaaekqepi
      721 ikcfrsleyi fkfiiqsrll faratagqye dsfkrdlycv faalnkmlgi pyemvlnsqm
      781 allysispvf eqlvavlpvl evakltctml dsvprepplp ltqakltaik nlttsslfre
      841 ddesrnlllv tmcrhlrihl irreelrsct eifgeilsfl hkrgrdtnkv nnciqhdvet
      901 lclsildvli qtilivints gpvlgclvac ligllqllde yhysrlweel ahagerkplk
      961 dfllrvylvl rdlvrqevfp pdwlvirmqa nnvilkslqe laqplafrfs geyfdsqlws
     1021 tyfnlavayl tqpslqleqf sevkrekive kygdmrvfmg fqilsmwtql aerklefipg
     1081 mvgpflevtl vpeselrkat lhiffdmmec eqrargsfkt veselidkld ilisenkgdd
     1141 eyrqlfntme hlsavlldrv qsedptwkes gtafitsitr llerlldyrs viqgdenrdk
     1201 rmsctvnlln fyknefnrke mylryiyklh dlhlaaenyt eagftmklya dqlgwgstil
     1261 ppdhshpqqp ewqrkevlyh kiihyldrgk cwekgiplck elavlyetrl ydyaklshvl
     1321 klqakfldni ltqlrpepey frvgfyglsf plfvrnklfi yrgleyerig aftqrlqtef
     1381 psaqilmkns ppdesiltse gqyiqicnvk pipeesslac rgaevpervv afylvndvrk
     1441 fifdrplhrg pvdrenefks lwierttltt eaklpgilrw feviekrsel lapvqyacet
     1501 mqsverelrr lvaqytaeph rninpfsmrl qgiidanvmg gitkyqeafl tpefarqnpd
     1561 mvphvnrlkg lildqmsvle aglnlhgqia pagvqplhkr lnerftqlkq glgplarqrt
     1621 ihhdsivnsp lpplptnekq rpatletags ryshadsdgl pedegfytkv dggpppipqr
     1681 evrprsvgyg ttpprpthqr slskplspkl plrhslptpt dgvdqtglrt swsepgpepa
     1741 pplpprgctp dkrdsntsti vppappkrla ykrntewstd ddseaqnepn dlrdsgyedf
     1801 eprsrcndim nisppsvina lnvstsnfas gmfqgshslp gqevspppip pkahqdtpsa
     1861 pstlervsnr tqlhghseny svpklqtlsm asdtestv
//
LOCUS       XP_034186713            1742 aa            linear   INV 03-SEP-2025
DEFINITION  dedicator of cytokinesis spg isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034186713
VERSION     XP_034186713.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330822.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1742
                     /product="dedicator of cytokinesis spg isoform X6"
                     /calculated_mol_wt=198278
     Region          <1..235
                     /region_name="DOCK_N"
                     /note="DOCK N-terminus; pfam16172"
                     /db_xref="CDD:465040"
     Region          243..427
                     /region_name="C2"
                     /note="C2 domain; cl14603"
                     /db_xref="CDD:472691"
     Region          1044..1436
                     /region_name="DHR2_DOCK"
                     /note="Dock Homology Region 2, a GEF domain, of Dedicator
                     of Cytokinesis proteins; cl06123"
                     /db_xref="CDD:471388"
     Site            order(1124,1135,1137..1138,1140..1141,1144..1145,
                     1147..1148,1151..1152)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1163,1165,1189,1192..1195,1197..1198,1211..1213,
                     1230..1232,1267..1270,1281..1282,1285,1287,1324..1325,
                     1328,1348..1349,1351..1352,1355..1357,1360..1362,
                     1365..1366,1369,1399,1420..1421,1424)
                     /site_type="other"
                     /note="Rac/Cdc42 binding site [polypeptide binding]"
                     /db_xref="CDD:212566"
     Site            order(1360..1362,1365)
                     /site_type="other"
                     /note="nucleotide sensor"
                     /db_xref="CDD:212566"
     CDS             1..1742
                     /gene="spg"
                     /coded_by="XM_034330822.2:63..5291"
                     /db_xref="GeneID:117607282"
ORIGIN      
        1 mvdpdtmsvv elyhvhvqsa ensqgasarg tlrrkehkkv lthhlyfcmr dfghsigedt
       61 eiyfslydak rneylserfl vriskegfss fiekihsnct iftdlgnadl srdlymvahv
      121 mrcgrmlysd sgknkagtat yrrphgvavl slaeatqdht eelemtfkvc qgeekefhql
      181 heqiirnnkc splpgqpnyg ivvslrvlhg elsqvreenp llfknicltk klgfsdvimp
      241 gdvrndlylk lergefergg kstgknievt ilvldadgkp legclfgaag tedsseyqsl
      301 iiyhhnspsw aetvrlaipi dkfygshvrf efrhcstrek tdkklfafaf vrlmepggat
      361 lqdglhelyi ykcedrskld slsyltlpts arepnatgtp afsrspkeav fvhtllcstk
      421 ltqnvdllsl lqwkahperi sealgrvlrl dgeelvkflq dildalfsmf htedgnstah
      481 sglvfqvlvs ifslledskf ehfkpvmday isghfaaalv ykgllssvqh cadwvtaaek
      541 qepiikcfrs leyifkfiiq srllfarata gqyedsfkrd lycvfaalnk mlgipyemvl
      601 nsqmallysi spvfeqlvav lpvlevaklt ctmldsvpre pplpltqakl taiknlttss
      661 lfreddesrn lllvtmcrhl rihlirreel rscteifgei lsflhkrgrd tnkvnnciqh
      721 dvetlclsil dvliqtiliv intsgpvlgc lvacligllq lldeyhysrl weelahager
      781 kplkdfllrv ylvlrdlvrq evfppdwlvi rmqannvilk slqelaqpla frfsgeyfds
      841 qlwstyfnla vayltqpslq leqfsevkre kivekygdmr vfmgfqilsm wtqlaerkle
      901 fipgmvgpfl evtlvpesel rkatlhiffd mmeceqrarg sfktveseli dkldilisen
      961 kgddeyrqlf ntmehlsavl ldrvqsedpt wkesgtafit sitrllerll dyrsviqgde
     1021 nrdkrmsctv nllnfyknef nrkemylryi yklhdlhlaa enyteagftm klyadqlgwg
     1081 stilppdhsh pqqpewqrke vlyhkiihyl drgkcwekgi plckelavly etrlydyakl
     1141 shvlklqakf ldniltqlrp epeyfrvgfy glsfplfvrn klfiyrgley erigaftqrl
     1201 qtefpsaqil mknsppdesi ltsegqyiqi cnvkpipees slacrgaevp ervvafylvn
     1261 dvrkfifdrp lhrgpvdren efkslwiert tltteaklpg ilrwfeviek rsellapvqy
     1321 acetmqsver elrrlvaqyt aephrninpf smrlqgiida nvmggitkyq eafltpefar
     1381 qnpdmvphvn rlkglildqm svleaglnlh gqiapagvqp lhkrlnerft qlkqglgpla
     1441 rqrtihhdsi vnsplpplpt nekqrpatle tagsryshad sdglpedegf ytkvdggppp
     1501 ipqrevrprs vgygttpprp thqrslskpl spklplrhsl ptptdgvdqt glrtswsepg
     1561 pepapplppr gctpdkrdsn tstivppapp krlaykrnte wstdddseaq nepndlrdsg
     1621 istaslldfq shltnlnnlg yedfeprsrc ndimnispps vinalnvsts nfasgmfqgs
     1681 hslpgqevsp ppippkahqd tpsapstler vsnrtqlhgh senysvpklq tlsmasdtes
     1741 tv
//
LOCUS       XP_034186714             979 aa            linear   INV 03-SEP-2025
DEFINITION  cilia- and flagella-associated protein 69-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186714
VERSION     XP_034186714.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330823.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..979
                     /product="cilia- and flagella-associated protein 69-like
                     isoform X1"
                     /calculated_mol_wt=113572
     CDS             1..979
                     /gene="LOC117607283"
                     /coded_by="XM_034330823.2:276..3215"
                     /db_xref="GeneID:117607283"
ORIGIN      
        1 mdrcednvlv idkleatdvw kesncpvyit pncaetdkis kelfdfdghs kfdvdyilqk
       61 ldelisdpvt stsvlricrf lhdyfyyigt ggykikhlpi iiklleflaq nvesvkeyei
      121 hlnqmlelcn lppileksse affnsdimeq yftilghllv ilpkqeqilk vhkalhslll
      181 kkkskntaai klklchtave ksqlpiimte llkssspdmy pkllevvfll ssisygcshk
      241 mietgilnii lirmdlpyat qlrctrppds lligneypee ttllimntlw slmksvlppn
      301 vvpihwkdnl psvhcalwgl cysferqvcy sqcrsinvqi rneiaviilv ilitfpswnl
      361 vssgiaedvi kffvgvetgt lrvfaenikf grsnedlffq killliithl aevdaciflm
      421 kekklmrtvl hlvnpntkey kitwnasqfw nlwtyainal svlvpkmpte fmeyngtlrl
      481 fiilewclst dynikivqnw tktictiiln nnlilledfr ehgvtlllik linyiltfdk
      541 itmkdqrilt lalisierlm kkqrfyqemy gdqsitltme llfrciyqkd keiqvdqrll
      601 laigsyiwec ilwcpknlek figyggvyii ldiieiipyp vqcvflgvft dmcdnyfcgp
      661 ylctwrgidk ktglmsllar mwreeeirvk vkrfpdgsvt deelpqmsnr qwsdtyrikl
      721 tqnispaiid migsvrskiy sirkiiegdq kryqhakehy kilyadlsme dritifnmdl
      781 yfkiklgqiw veiskyfeqa gitplgmdgq alflmtqryy swgkliknge qkiiksvqia
      841 edieekdeya rirdaklila ldafdeldyi yrttnrsyll ekkdkqirqv naalnfppna
      901 adahchrtff hkppitaiyn qhpviksslr sdsnfnqvkc lpvspccsfi sdnvcsseis
      961 lpiitsscil kpeefiedv
//
LOCUS       XP_034186715             979 aa            linear   INV 03-SEP-2025
DEFINITION  cilia- and flagella-associated protein 69-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186715
VERSION     XP_034186715.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330824.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..979
                     /product="cilia- and flagella-associated protein 69-like
                     isoform X1"
                     /calculated_mol_wt=113572
     CDS             1..979
                     /gene="LOC117607283"
                     /coded_by="XM_034330824.2:343..3282"
                     /db_xref="GeneID:117607283"
ORIGIN      
        1 mdrcednvlv idkleatdvw kesncpvyit pncaetdkis kelfdfdghs kfdvdyilqk
       61 ldelisdpvt stsvlricrf lhdyfyyigt ggykikhlpi iiklleflaq nvesvkeyei
      121 hlnqmlelcn lppileksse affnsdimeq yftilghllv ilpkqeqilk vhkalhslll
      181 kkkskntaai klklchtave ksqlpiimte llkssspdmy pkllevvfll ssisygcshk
      241 mietgilnii lirmdlpyat qlrctrppds lligneypee ttllimntlw slmksvlppn
      301 vvpihwkdnl psvhcalwgl cysferqvcy sqcrsinvqi rneiaviilv ilitfpswnl
      361 vssgiaedvi kffvgvetgt lrvfaenikf grsnedlffq killliithl aevdaciflm
      421 kekklmrtvl hlvnpntkey kitwnasqfw nlwtyainal svlvpkmpte fmeyngtlrl
      481 fiilewclst dynikivqnw tktictiiln nnlilledfr ehgvtlllik linyiltfdk
      541 itmkdqrilt lalisierlm kkqrfyqemy gdqsitltme llfrciyqkd keiqvdqrll
      601 laigsyiwec ilwcpknlek figyggvyii ldiieiipyp vqcvflgvft dmcdnyfcgp
      661 ylctwrgidk ktglmsllar mwreeeirvk vkrfpdgsvt deelpqmsnr qwsdtyrikl
      721 tqnispaiid migsvrskiy sirkiiegdq kryqhakehy kilyadlsme dritifnmdl
      781 yfkiklgqiw veiskyfeqa gitplgmdgq alflmtqryy swgkliknge qkiiksvqia
      841 edieekdeya rirdaklila ldafdeldyi yrttnrsyll ekkdkqirqv naalnfppna
      901 adahchrtff hkppitaiyn qhpviksslr sdsnfnqvkc lpvspccsfi sdnvcsseis
      961 lpiitsscil kpeefiedv
//
LOCUS       XP_034186716             728 aa            linear   INV 03-SEP-2025
DEFINITION  cilia- and flagella-associated protein 69-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186716
VERSION     XP_034186716.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330825.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..728
                     /product="cilia- and flagella-associated protein 69-like
                     isoform X2"
                     /calculated_mol_wt=84784
     CDS             1..728
                     /gene="LOC117607283"
                     /coded_by="XM_034330825.2:129..2315"
                     /db_xref="GeneID:117607283"
ORIGIN      
        1 mlmdlpyatq lrctrppdsl ligneypeet tllimntlws lmksvlppnv vpihwkdnlp
       61 svhcalwglc ysferqvcys qcrsinvqir neiaviilvi litfpswnlv ssgiaedvik
      121 ffvgvetgtl rvfaenikfg rsnedlffqk illliithla evdaciflmk ekklmrtvlh
      181 lvnpntkeyk itwnasqfwn lwtyainals vlvpkmptef meyngtlrlf iilewclstd
      241 ynikivqnwt ktictiilnn nlilledfre hgvtlllikl inyiltfdki tmkdqriltl
      301 alisierlmk kqrfyqemyg dqsitltmel lfrciyqkdk eiqvdqrlll aigsyiweci
      361 lwcpknlekf igyggvyiil diieiipypv qcvflgvftd mcdnyfcgpy lctwrgidkk
      421 tglmsllarm wreeeirvkv krfpdgsvtd eelpqmsnrq wsdtyriklt qnispaiidm
      481 igsvrskiys irkiiegdqk ryqhakehyk ilyadlsmed ritifnmdly fkiklgqiwv
      541 eiskyfeqag itplgmdgqa lflmtqryys wgklikngeq kiiksvqiae dieekdeyar
      601 irdaklilal dafdeldyiy rttnrsylle kkdkqirqvn aalnfppnaa dahchrtffh
      661 kppitaiynq hpviksslrs dsnfnqvkcl pvspccsfis dnvcsseisl piitsscilk
      721 peefiedv
//
LOCUS       XP_034186717             875 aa            linear   INV 03-SEP-2025
DEFINITION  coat protein (coatomer) gamma [Osmia lignaria lignaria].
ACCESSION   XP_034186717
VERSION     XP_034186717.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330826.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..875
                     /product="coat protein (coatomer) gamma"
                     /calculated_mol_wt=96273
     Region          1..871
                     /region_name="SEC21"
                     /note="Vesicle coat complex COPI, gamma subunit
                     [Intracellular trafficking and secretion]; COG5240"
                     /db_xref="CDD:227565"
     Region          211..238
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          248..277
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          285..309
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          319..346
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          396..421
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Site            order(413..414,417,420..421,448..449,452,455..456,459,
                     487..488,491,494..495,498,524..525,528,534..535)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:293787"
     Region          433..459
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          469..499
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     Region          508..535
                     /region_name="HEAT repeat"
                     /note="HEAT repeat [structural motif]"
                     /db_xref="CDD:293787"
     CDS             1..875
                     /gene="gammaCOP"
                     /coded_by="XM_034330826.2:95..2722"
                     /db_xref="GeneID:117607284"
ORIGIN      
        1 mnafkrdkke eedgggnpfq nlekttvlqe artfndtpvn prkcahiltk ilyllnqgeq
       61 lstmeateaf famtklfqsr dvvlrrlvyl gikelsslae dviivtsslt kdmtgkedly
      121 raaairalct itdggmlaai erymkqaivd rspavssaal vsslhltnvs gdvarrwane
      181 aqealnsnnv mvqyhalgvl yqarkadkha viklfaklmr tspkspyagc mliriackll
      241 devderyell kfiecclrhk semvvyeaah alvnlggcgs rdissaisvl qlfcgspkpa
      301 lrfaavrtln kvamthpaev tacnldlenl itdsnrsiat laittllktg aessvdrlmk
      361 qiatfvseis defkvvvvqa iralcqkfpr khavlmnfls amlrdeggle ykaaiadtii
      421 avmegnaeak eaglahlcef iedcehisla vrilhllgqe gptskqpsry irfiynrvil
      481 esasvraaav talarfaaac ppllpnvlvl lsrcqldsdd evrdraayyc tilqqqndli
      541 vlplvqppll svpsleralr nymrtpmdep fdisqippaq taeepaqvev hatvkqqprl
      601 treesfmekl sqiphlsmii rdssllkssp vfeltesete ynvkcvkhtf tdllvlqfdc
      661 mntlsdqlle dvrvaveppe gyrivcevpc prlpynepgt tytvlkyped vhasvatipt
      721 tlrfmardcd patgvpdaeq gyndeymled levtladqvr gmgnggldfg aaweaaaakg
      781 ftkleetfal gpsvtslkga iqsltgflgl epvdrtnrvq sgavshnlll sgifrggkev
      841 fararlalsd sqvtmqlsvl cqdpdvaeli vssvg
//
LOCUS       XP_034186718             636 aa            linear   INV 03-SEP-2025
DEFINITION  plexin domain containing lethal (1) G0289 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186718
VERSION     XP_034186718.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330827.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..636
                     /product="plexin domain containing lethal (1) G0289"
                     /calculated_mol_wt=72186
     Region          468..508
                     /region_name="PSI"
                     /note="Plexin repeat; pfam01437"
                     /db_xref="CDD:396154"
     CDS             1..636
                     /gene="l(1)G0289"
                     /coded_by="XM_034330827.2:538..2448"
                     /db_xref="GeneID:117607285"
ORIGIN      
        1 maceqrwcfn rgcdganrrw lyilllafcv lqhldssvan neldryhygl fgghsnseig
       61 piefsyvsve dpsenvqiid rrrretdeks pptteqtpqe qqqqqsqepq qqqpaatehp
      121 esparkpppk vvelrksrpn vsysmestte ssvvssprpt awfvkqeptt tekpsnvtsl
      181 tvesvplkps tknmksnatd kvaetnknat nvtesavtas tvekddsesn igdisiskfs
      241 dvtnktlsqh nitkteldth qyynstfiid eavgrrywvd inknpdlkvn hllsqshrra
      301 atvklkfdfp fyghkvrnit iatggflytg eyvhswlaat qyiaplmanf dtrlsnesyv
      361 kyadngtaft vvwekvvlqd kpdagaftfq vtlhqngdiv fvysmiplmv ekiqdtahpv
      421 kvglsdayim drivffvrrk tiyeyhrvnf nrqdiknwtv iylnalptcl emdnckdclt
      481 klkdfdckwc selnqcstgt frsrqdwllk gcdvrkikdv nncpspttty keheedfnhv
      541 lhvhsektvg sdlmnakqer pgtsplgrsr dnmnmgvsgi igillvialv vglaawagya
      601 yrnphsasgq mliryrpsqw swrrgearyt aatihm
//
LOCUS       XP_034186719             507 aa            linear   INV 03-SEP-2025
DEFINITION  dihydrolipoyl dehydrogenase, mitochondrial [Osmia lignaria
            lignaria].
ACCESSION   XP_034186719
VERSION     XP_034186719.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330828.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="dihydrolipoyl dehydrogenase, mitochondrial"
                     /calculated_mol_wt=53748
     Region          53..507
                     /region_name="Pyr_redox_2"
                     /note="Pyridine nucleotide-disulphide oxidoreductase;
                     cl39093"
                     /db_xref="CDD:476868"
     CDS             1..507
                     /gene="LOC117607286"
                     /coded_by="XM_034330828.2:146..1669"
                     /db_xref="GeneID:117607286"
ORIGIN      
        1 mqatfwslvt tsvrptcvkr vvpgltaaqq rrysstldad vvvigggpgg yvasikaaql
       61 gmktvcvekd etlggtclnv gcipskslln nshyyhmahs gdlknrgvlv envkldldkl
      121 meqkrtvvka ltsgiaglfk knkvewvkgh gkisgqnqvt alkpdgsvas tintkniiia
      181 tgsevtpfpg ieidekqvvs stgalslsqv pkrlivigag viglelgsvw qrlgsqvtav
      241 efmpsiggag idaevsqtlq kilskqgvgf klgtkvtaak ktgneivvsv edakdpskke
      301 divcdvllvc vgrrpytsnl gledmgierd ekgripvnnr fqtvvpsifa igdcihgpml
      361 ahkaeeegii tvegiaggav hidyncvpsv iythpevgwv gkteedlkke giaykvgkfp
      421 hlansraktn letdgfvkvl adsatdkvlg vhmigsvage lineavlame ygasaedvsr
      481 tchahptcae alkeahlaay fgkpinf
//
LOCUS       XP_034186721             475 aa            linear   INV 03-SEP-2025
DEFINITION  methionyl aminopeptidase und [Osmia lignaria lignaria].
ACCESSION   XP_034186721
VERSION     XP_034186721.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330830.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="methionyl aminopeptidase und"
                     /calculated_mol_wt=52191
     Region          7..475
                     /region_name="PTZ00053"
                     /note="methionine aminopeptidase 2; Provisional"
                     /db_xref="CDD:240246"
     CDS             1..475
                     /gene="und"
                     /coded_by="XM_034330830.2:67..1494"
                     /db_xref="GeneID:117607287"
ORIGIN      
        1 maavldevgk saeklvneek neivdeedea gaveaskkkk kkkkkkkaga geasadvpeg
       61 eedkakednt vgedggaepa endadtedak kkkkkrkprg kggakqqtdp ptipvaelfp
      121 dgnfpvgqim dhpsaagvdd rtakerfsse earafdrmhn diynearqaa eahrqtrkhi
      181 mkwvrpgmtm ieicnelent arkligedgf kaglafptgc srnhcaahyt pnagdptvle
      241 yddvtkidfg thingriidc aftlafnpky dklieavrda tntgikaagi dvqlcdvgaa
      301 iqevmesyev eldgktyqvk sirnlnghsi apyrihagkt vpivkggeat rmeenefyai
      361 etfgstgrgi vhddldcshy mksfdagfvp lrlqssksll nvinkhfgtl afckrwldrv
      421 gctkyqmalk dlcdkgavea ypplvdvkgc ytaqfehtlv lrptckevis rgddy
//
LOCUS       XP_034186722             396 aa            linear   INV 03-SEP-2025
DEFINITION  innexin 3 [Osmia lignaria lignaria].
ACCESSION   XP_034186722
VERSION     XP_034186722.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330831.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="innexin 3"
                     /calculated_mol_wt=44640
     Region          27..357
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..396
                     /gene="Inx3"
                     /coded_by="XM_034330831.2:580..1770"
                     /db_xref="GeneID:117607288"
ORIGIN      
        1 mavfglvsav agfvkvrylv dkavidnmif rahyritsai lfacciivsa nnllgdpinc
       61 lsdggvpeav intycwityt ftlphnnakp vgthvahpgl gtdigekryh syyqwvpfml
      121 ffqgilfyip hwiwkqweeg kvrmisegmr galvdnkper qaksqrlvky iydtlhlhnt
      181 yaagyffcea lnfvnvvsni ffidtflgga flsygtdvlk fsnmnqeqri dpmievfprv
      241 tkctfhkfga sgtiqkfdal cvlalnilne kiyiflwfwf iilavlsgva llysmavvll
      301 pstretilrk rfkfgtpagv salirktqvg dflllhllgq nmnimmfnev ldelcrqlhl
      361 gstsgaspts vpsapstlem spiypeieky akdtei
//
LOCUS       XP_034186723             396 aa            linear   INV 03-SEP-2025
DEFINITION  innexin 3 [Osmia lignaria lignaria].
ACCESSION   XP_034186723
VERSION     XP_034186723.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330832.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="innexin 3"
                     /calculated_mol_wt=44640
     Region          27..357
                     /region_name="Innexin"
                     /note="pfam00876"
                     /db_xref="CDD:459975"
     CDS             1..396
                     /gene="Inx3"
                     /coded_by="XM_034330832.2:410..1600"
                     /db_xref="GeneID:117607288"
ORIGIN      
        1 mavfglvsav agfvkvrylv dkavidnmif rahyritsai lfacciivsa nnllgdpinc
       61 lsdggvpeav intycwityt ftlphnnakp vgthvahpgl gtdigekryh syyqwvpfml
      121 ffqgilfyip hwiwkqweeg kvrmisegmr galvdnkper qaksqrlvky iydtlhlhnt
      181 yaagyffcea lnfvnvvsni ffidtflgga flsygtdvlk fsnmnqeqri dpmievfprv
      241 tkctfhkfga sgtiqkfdal cvlalnilne kiyiflwfwf iilavlsgva llysmavvll
      301 pstretilrk rfkfgtpagv salirktqvg dflllhllgq nmnimmfnev ldelcrqlhl
      361 gstsgaspts vpsapstlem spiypeieky akdtei
//
LOCUS       XP_034186724             287 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607289 [Osmia lignaria lignaria].
ACCESSION   XP_034186724
VERSION     XP_034186724.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330833.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186724.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="uncharacterized protein LOC117607289"
                     /calculated_mol_wt=32222
     Region          170..282
                     /region_name="DUF1151"
                     /note="Protein of unknown function (DUF1151); pfam06625"
                     /db_xref="CDD:461966"
     CDS             1..287
                     /gene="LOC117607289"
                     /coded_by="XM_034330833.2:402..1265"
                     /db_xref="GeneID:117607289"
ORIGIN      
        1 mceivkgqrr afrdqdppri rdrseenvkt eiefskstpn lpivaqgass fekcssepef
       61 lkkdrenrkm ergdlssqgs tstdsirvsd nvkvvetvdq pdvpnrmsse hflkeladkv
      121 pssgrhdnev itngvknetd scdgltngkk mapvlgvppp pppaphmgpd glilprkpyn
      181 pyltssnhkd lrrellfnqk vgknvlnqks elqralekqr eaasrreaek nreenykddp
      241 rtalqraieq rakhiemtle qsqppsepas nllatarakl rprtdsq
//
LOCUS       XP_034191031            1640 aa            linear   INV 03-SEP-2025
DEFINITION  macroglobulin complement-related isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191031
VERSION     XP_034191031.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335140.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1640
                     /product="macroglobulin complement-related isoform X1"
                     /calculated_mol_wt=187823
     Region          <166..1457
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          1035..1372
                     /region_name="TED_complement"
                     /note="A-macroglobulin TED domain; pfam07678"
                     /db_xref="CDD:462227"
     Region          1488..1581
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1640
                     /gene="Mcr"
                     /coded_by="XM_034335140.2:146..5068"
                     /db_xref="GeneID:117609163"
ORIGIN      
        1 mkkkksnste kmwmwiwili ltfacgnsql sqestestrl pwnrfddrdp svygtrntdr
       61 lgdnvlikea tyfvvaskmv rpgqiyrvav nvlyspipmt irasiqrdgv eiasdyqevk
      121 edipqtlmmk mpptsvggey klrvegtyns ltggqaflne tklifsqrsm sifiqldkpv
      181 ymqketirfr tipintelka fnnpidvyml dpyrrimrrw lsrqsnlgtv slsyqlsdqp
      241 vfgewiiqvi aqgqveektf lveeyyqtrf evnvtmpaff fdndpyiygt vqanytsgap
      301 vrgnltlkat fksldrrspd vapesieryf yfdeyypswl kipsyfdnki pvlrffngty
      361 hfkypmsell tfvpaasgae itvtatvger fleeiisgys tarifnsttk irflggtpqv
      421 fkpampfsvn lvasfhdnsv lrpsqlkgsv meiradietr tggrrnletq ylkptpdnag
      481 iwstridlrk qlgldhssdh aqqilndiss mrvyayltdg egfrtqtell llahespnqq
      541 qikiststek pkvgeymifh iqtnfyidtf nyiimakgii lltgqdsmqh nvktiavpls
      601 aemapvativ vyhigqysnv iadsltfsvn gisrnnftvy innkkartge nvevaiygep
      661 gayvalsgid rafytmqagn eltyanvitk mahfdeetng tqshtwlyhd gdpdelvyfp
      721 sstfgidvnr tfeyiglvvf tdafvhrrpd ncnatlglge clsgkcyrld kkcdgtfdce
      781 dgtdeagceh rnltdvatfr kwrfnrvqrl yenvwlwkdi nigphgryif nidvprrpvq
      841 wmitafsmsp smgfgmlpka ieymgilpfy invempthsr qgeqigirls vfnylrynie
      901 avvvlagskd ykfvhvedng ivqsykprts fgdhqffiwi paqdaaivyl pivpvrlgdi
      961 kvhiyattvi grdsvtrnlh veadglpqhr hqsilldlsn rayvfqymhv nitetpiipy
     1021 denryyvfgs nkavislvgd vvgpifptmp vnatslmdlp mdcaeqnmfs favnlyttmy
     1081 mrlinqrnrt qekesfyymn igyqrqlsfm npdgsfslfr sdwnqsapsv wltaycarvl
     1141 qearfyewen ylyidpevia navswilrhq taegsfyevr wlpdrkmnss lnydydiith
     1201 rnisltahvl itlqsvkdlp eglgtqvavs aanavkwler nlklledrgk pydiaivsya
     1261 lllakastag qafnilskha rreggltywg reqvplppyk lenqkpfllp rlpykydsen
     1321 iettayallv hvarqeiiie pivkwlnsqr ltdggwastq dtawamkalm dytvrsrird
     1381 vsslavtvea tslpgqtktl fvndnnlarl qtieipeawg tvkvqakgag yailqmsvqy
     1441 nvdiakfqtq ppvkafdlvt ranfhgrnqs hisylscqrw tntnesarsg mavldvaipt
     1501 gyiiqqqtld ryilskqvrn lhrarfqekk vlfyfdylde eetcvnftie rwfpvanmsr
     1561 yhpirvydyy aperfnetif dalptytlni cevcgssqcp ycpiynaatl lstptgilli
     1621 vsllivssry frmqefiiri
//
LOCUS       XP_034191032            1639 aa            linear   INV 03-SEP-2025
DEFINITION  macroglobulin complement-related isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191032
VERSION     XP_034191032.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335141.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1639
                     /product="macroglobulin complement-related isoform X2"
                     /calculated_mol_wt=187564
     Region          <166..1457
                     /region_name="YfaS"
                     /note="Uncharacterized conserved protein YfaS,
                     alpha-2-macroglobulin family [General function prediction
                     only]; COG2373"
                     /db_xref="CDD:441940"
     Region          1035..1372
                     /region_name="TED_complement"
                     /note="A-macroglobulin TED domain; pfam07678"
                     /db_xref="CDD:462227"
     Region          1488..1581
                     /region_name="A2M_recep"
                     /note="A-macroglobulin receptor binding domain; pfam07677"
                     /db_xref="CDD:462226"
     CDS             1..1639
                     /gene="Mcr"
                     /coded_by="XM_034335141.2:146..5065"
                     /db_xref="GeneID:117609163"
ORIGIN      
        1 mkkkksnste kmwmwiwili ltfacgnsql sqestestrl pwnrfddrdp svygtrntdr
       61 lgdnvlikea tyfvvaskmv rpgqiyrvav nvlyspipmt irasiqrdgv eiasdyqevk
      121 edipqtlmmk mpptsvggey klrvegtyns ltggqaflne tklifsqrsm sifiqldkpv
      181 ymqketirfr tipintelka fnnpidvyml dpyrrimrrw lsrqsnlgtv slsyqlsdqp
      241 vfgewiiqvi aqgqveektf lveeyyqtrf evnvtmpaff fdndpyiygt vqanytsgap
      301 vrgnltlkat fksldrrspd vapesieryf yfdeyypswl kipsyfdnki pvlrffngty
      361 hfkypmsell tfvpaasgae itvtatvger fleeiisgys tarifnsttk irflggtpqv
      421 fkpampfsvn lvasfhdnsv lrpsqlkgsv meiradietr tggrrnletq ylkptpdnag
      481 iwstridlrk qlgldhssdh aqqilndiss mrvyayltdg egfrtqtell llahespnqq
      541 qikiststek pkvgeymifh iqtnfyidtf nyiimakgii lltgqdsmqh nvktiavpls
      601 aemapvativ vyhigqysnv iadsltfsvn gisrnnftvy innkkartge nvevaiygep
      661 gayvalsgid rafytmqagn eltyanvitk mahfdeetng tqshtwlyhd gdpdelvyfp
      721 sstfgidvnr tfeyiglvvf tdafvhrrpd ncnatlglge clsgkcyrld kkcdgtfdce
      781 dgtdeagceh rnltdvatfr kwrfnrvqrl yenvwlwkdi nigphgryif nidvprrpvq
      841 wmitafsmsp smgfgmlpka ieymgilpfy invempthsr qgeqigirls vfnylrynie
      901 avvvlagskd ykfvhvedng ivqsykprts fgdhqffiwi paqdaaivyl pivpvrlgdi
      961 kvhiyattvi grdsvtrnlh veadglpqhr hqsilldlsn rayvfqymhv nitetpiipy
     1021 denryyvfgs nkavislvgd vvgpifptmp vnatslmdlp mdcaeqnmfs favnlyttmy
     1081 mrlinqrnrt qekesfyymn igyqrqlsfm npdgsfslfr sdwnqsapsv wltaycarvl
     1141 qearfyewen ylyidpevia navswilrhq taegsfyevr wlpdrkmnss lnydydiith
     1201 rnisltahvl itlqsvkdlp eglgtqvavs aanavkwler nlklledrgk pydiaivsya
     1261 lllakastag qafnilskha rreggltywg reqvplppyk lenqkpfllp rlpykydsen
     1321 iettayallv hvarqeiiie pivkwlnsqr ltdggwastq dtawamkalm dytvrsrird
     1381 vsslavtvea tslpgqtktl fvndnnlarl qtieipeawg tvkvqakgag yailqmsvqy
     1441 nvdiakfqtq ppvkafdlvt ranfhgrnqs hisylscqrw tntnesarsg mavldvaipt
     1501 gyiiqqqtld ryilskqvrn lhrarfqekk vlfyfdylde eetcvnftie rwfpvanmsr
     1561 yhpirvydyy aperfnetif dalptytlni cevcgssqcp ycpiynaatl lstptgmekt
     1621 rptnifeaif niaiksvlq
//
LOCUS       XP_034191033            1137 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional regulator additional sex combs isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191033
VERSION     XP_034191033.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335142.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191033.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1137
                     /product="transcriptional regulator additional sex combs
                     isoform X1"
                     /calculated_mol_wt=123985
     Region          111..233
                     /region_name="ASXH"
                     /note="Asx homology domain; pfam13919"
                     /db_xref="CDD:464041"
     Region          <759..960
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          <1095..1136
                     /region_name="PHD_3"
                     /note="PHD domain of transcriptional enhancer, Asx;
                     pfam13922"
                     /db_xref="CDD:316444"
     CDS             1..1137
                     /gene="Asx"
                     /coded_by="XM_034335142.2:348..3761"
                     /db_xref="GeneID:117609164"
ORIGIN      
        1 mdtdvelakg egcetspgcs gyssmvhskk vikhalrqqa krrrknttia agnsrtlpri
       61 vvkplppppp ndppspvnnv qhinttteep aatmrevlas lpgfslkpgr rrstkrlsat
      121 aqleaglvdl espasilast slrallnrht fqglpplyqr klaqllpavd rqdaaisgln
      181 neffaracle wrkrlaegef tpenqqrlkm eaerdknkld pwkvkhfepi wgekrepkfr
      241 sglhcilesr sggavtrssl rlrlesnvdt pvldapcpvi isedkieedn ckvetginns
      301 delndttgip ellpahynea thasidekhi esvnissvet iadaemhqdk leeddkvinl
      361 tekqdtieek eavtqvcqek vsntyddeih lpqsekslqp menhllevse edtmllpeet
      421 tsprsseqme qishtsrets sqlsvectpq ssvdqvpqis keqisqmsee qiypmsdcet
      481 ttmletspph eqvrpteivm edstlinnnq settqithen padgesqipe gmeidsetlq
      541 rihelevrge mreayeeisg cpeeimypil dgmemgsnsa eetetqvvsg pseaprdqqd
      601 vnngnedeal reannyvcse mlecswpvdp tvnnisnnnr tqeelqvpwp lvaaaldgsv
      661 aanitvttqd ecndaptstd stnppqqfin adsnvesmnc iqlpvvqgtp fqseglaigt
      721 pgtscimknf qsqsspiiaf pqlqsirfvq tsfhsdqntt ptlnstspit aqiqnqqnts
      781 sqvnimrtqe eivqlnaqtn ntrnirnnvt qnqvsntvta aigvqpqsra qntiviqhqt
      841 sastqprqvv atiqqqqqqq qqypgatvav ssrssrssnq gsqrgsrssn keqgggrsrs
      901 stkeppgavn lersyqicqa viqsspnrdq lkahlkppps llargdgaft tnksggrtit
      961 tvktqkpsqt iqnkqaqnkt qavmlrhvfa tarqatsaev pesntvaqlg statsglgqy
     1021 ilvqrtgvgd saprassapp lppqiagmgv gvhlvrgrpa sagegshqav tlkargtdgr
     1081 ggggaepgap gmimggdppp pcecnmrgam vicrqcgafc hddcigpqri catclir
//
LOCUS       XP_034191034            1000 aa            linear   INV 03-SEP-2025
DEFINITION  transcriptional regulator additional sex combs isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191034
VERSION     XP_034191034.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335143.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191034.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1000
                     /product="transcriptional regulator additional sex combs
                     isoform X2"
                     /calculated_mol_wt=110303
     Region          111..233
                     /region_name="ASXH"
                     /note="Asx homology domain; pfam13919"
                     /db_xref="CDD:464041"
     Region          <759..960
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1000
                     /gene="Asx"
                     /coded_by="XM_034335143.2:348..3350"
                     /db_xref="GeneID:117609164"
ORIGIN      
        1 mdtdvelakg egcetspgcs gyssmvhskk vikhalrqqa krrrknttia agnsrtlpri
       61 vvkplppppp ndppspvnnv qhinttteep aatmrevlas lpgfslkpgr rrstkrlsat
      121 aqleaglvdl espasilast slrallnrht fqglpplyqr klaqllpavd rqdaaisgln
      181 neffaracle wrkrlaegef tpenqqrlkm eaerdknkld pwkvkhfepi wgekrepkfr
      241 sglhcilesr sggavtrssl rlrlesnvdt pvldapcpvi isedkieedn ckvetginns
      301 delndttgip ellpahynea thasidekhi esvnissvet iadaemhqdk leeddkvinl
      361 tekqdtieek eavtqvcqek vsntyddeih lpqsekslqp menhllevse edtmllpeet
      421 tsprsseqme qishtsrets sqlsvectpq ssvdqvpqis keqisqmsee qiypmsdcet
      481 ttmletspph eqvrpteivm edstlinnnq settqithen padgesqipe gmeidsetlq
      541 rihelevrge mreayeeisg cpeeimypil dgmemgsnsa eetetqvvsg pseaprdqqd
      601 vnngnedeal reannyvcse mlecswpvdp tvnnisnnnr tqeelqvpwp lvaaaldgsv
      661 aanitvttqd ecndaptstd stnppqqfin adsnvesmnc iqlpvvqgtp fqseglaigt
      721 pgtscimknf qsqsspiiaf pqlqsirfvq tsfhsdqntt ptlnstspit aqiqnqqnts
      781 sqvnimrtqe eivqlnaqtn ntrnirnnvt qnqvsntvta aigvqpqsra qntiviqhqt
      841 sastqprqvv atiqqqqqqq qqypgatvav ssrssrssnq gsqrgsrssn keqgggrsrs
      901 stkeppgavn lersyqicqa viqsspnrdq lkahlkppps llargdgaft tnksggrtit
      961 tvktqkpsqt iqnkqaqnkt qavmlrhvfa tarqatsaec
//
LOCUS       XP_034191035            1059 aa            linear   INV 03-SEP-2025
DEFINITION  retinoblastoma-like protein 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191035
VERSION     XP_034191035.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335144.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1059
                     /product="retinoblastoma-like protein 1 isoform X1"
                     /calculated_mol_wt=119479
     Region          94..229
                     /region_name="DUF3452"
                     /note="Domain of unknown function (DUF3452); pfam11934"
                     /db_xref="CDD:463402"
     Region          436..628
                     /region_name="RB_A"
                     /note="Retinoblastoma-associated protein A domain;
                     pfam01858"
                     /db_xref="CDD:460364"
     Region          769..936
                     /region_name="CYCLIN_SF"
                     /note="Cyclin box fold superfamily; cl40454"
                     /db_xref="CDD:477360"
     CDS             1..1059
                     /gene="LOC117609165"
                     /coded_by="XM_034335144.2:165..3344"
                     /db_xref="GeneID:117609165"
ORIGIN      
        1 mlyiidwrrw iiwwrwsfpt kmgqsddved stysrhqdlc qklnmdataa aeawksyeti
       61 rqnytlegdq lhwigcalyv acrkssiptv gktgtnvegn cvsltrllql cnlpliqfft
      121 kskswadman mpqdfrskie klegnfavsm vifkkyqpif tdvfkdpvdd isrphrsrrh
      181 kaipctparv fefcwtlfic ikgafpdisd dlvnsyhlll accdliysna llanrkdlln
      241 pnfpglpanf nddnytppqt anciitllce rheaiaveak vikeyylrnh inklfndrvl
      301 rgdqsnfsgi lealnfdgns kainkvyeqh vlsvgdfder ifladwrrvr lngissleiv
      361 ggniachvkl skhisikgnd asnnigsptq mmnvgdlqeq fqikreqysg qiqhlapptp
      421 ltgrnylksk ditnvtpvst atqsvirlqa mlagqtspse nllqilnscs qdaktlvesk
      481 vkeigeqfca nynkstnvnd ttsdfgkkrl ylgqtlfyrl lekilndekr kkpnydvtnl
      541 lmnevfiqcl faccleiviy syksndkifp wilnalnlda yyfykvieii vraedqlsrd
      601 vvkhlnqiee kileslawkn dsplwgaiqs tpegvpscee vslpgtletv dpnipgqpvl
      661 rrialdrgth hdvqqspiss aserfqspva asgvakkrlf pdtrisgqsv lrvagqnvlp
      721 skvlidgnsr illlpeltvp rssgvqatst pmqtvtvnre pvkpkrtgsv alffrkfynl
      781 asvrmldlcg sleimdidlk kkiwtifeys ikertelmkd rhldqilmca iyvicklakm
      841 eknsfteimr cyrlqpqaes hiyrsvliak tstdesqkts eesgqkdvpd kepnvapptp
      901 tnmagtsqnf geetrgdlik fyntvyvpqv kemanklgla rgsvtnlsls plpkgkppas
      961 spvrrvtssi mtrtldpkai saspapqlsy cfsrspakdl eainkmmisv dpkrsvgkrl
     1021 ltddtdvems eatspikktt afvarrleni igerrtqnq
//
LOCUS       XP_034191036            1025 aa            linear   INV 03-SEP-2025
DEFINITION  retinoblastoma-like protein 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191036
VERSION     XP_034191036.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335145.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1025
                     /product="retinoblastoma-like protein 1 isoform X2"
                     /calculated_mol_wt=115725
     Region          94..229
                     /region_name="DUF3452"
                     /note="Domain of unknown function (DUF3452); pfam11934"
                     /db_xref="CDD:463402"
     Region          402..594
                     /region_name="RB_A"
                     /note="Retinoblastoma-associated protein A domain;
                     pfam01858"
                     /db_xref="CDD:460364"
     Region          735..897
                     /region_name="RB_B"
                     /note="Retinoblastoma-associated protein B domain;
                     pfam01857"
                     /db_xref="CDD:460363"
     CDS             1..1025
                     /gene="LOC117609165"
                     /coded_by="XM_034335145.2:165..3242"
                     /db_xref="GeneID:117609165"
ORIGIN      
        1 mlyiidwrrw iiwwrwsfpt kmgqsddved stysrhqdlc qklnmdataa aeawksyeti
       61 rqnytlegdq lhwigcalyv acrkssiptv gktgtnvegn cvsltrllql cnlpliqfft
      121 kskswadman mpqdfrskie klegnfavsm vifkkyqpif tdvfkdpvdd isrphrsrrh
      181 kaipctparv fefcwtlfic ikgafpdisd dlvnsyhlll accdliysna llanrkdlln
      241 pnfpglpanf nddnytppqt anciitllce rheaiaveak vikeyylrnh inklfndrvl
      301 rgdqsnfsgi lealnfdgns kainkvyeqh vlsvgdfder iflgndasnn igsptqmmnv
      361 gdlqeqfqik reqysgqiqh lapptpltgr nylkskditn vtpvstatqs virlqamlag
      421 qtspsenllq ilnscsqdak tlveskvkei geqfcanynk stnvndttsd fgkkrlylgq
      481 tlfyrlleki lndekrkkpn ydvtnllmne vfiqclfacc leiviysyks ndkifpwiln
      541 alnldayyfy kvieiivrae dqlsrdvvkh lnqieekile slawkndspl wgaiqstpeg
      601 vpsceevslp gtletvdpni pgqpvlrria ldrgthhdvq qspissaser fqspvaasgv
      661 akkrlfpdtr isgqsvlrva gqnvlpskvl idgnsrilll peltvprssg vqatstpmqt
      721 vtvnrepvkp krtgsvalff rkfynlasvr mldlcgslei mdidlkkkiw tifeysiker
      781 telmkdrhld qilmcaiyvi cklakmekns fteimrcyrl qpqaeshiyr svliaktstd
      841 esqktseesg qkdvpdkepn vapptptnma gtsqnfgeet rgdlikfynt vyvpqvkema
      901 nklglargsv tnlslsplpk gkppasspvr rvtssimtrt ldpkaisasp apqlsycfsr
      961 spakdleain kmmisvdpkr svgkrlltdd tdvemseats pikkttafva rrleniiger
     1021 rtqnq
//
LOCUS       XP_034191039             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191039
VERSION     XP_034191039.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335148.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191039.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335148.2:287..3064"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191038             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191038
VERSION     XP_034191038.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335147.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191038.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335147.2:146..2923"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191037             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191037
VERSION     XP_034191037.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335146.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191037.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335146.2:198..2975"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191040             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191040
VERSION     XP_034191040.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335149.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191040.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335149.2:257..3034"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191042             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191042
VERSION     XP_034191042.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335151.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191042.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335151.2:154..2931"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191043             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191043
VERSION     XP_034191043.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335152.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191043.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335152.2:351..3128"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191044             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191044
VERSION     XP_034191044.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335153.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191044.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335153.2:123..2900"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191045             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191045
VERSION     XP_034191045.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335154.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191045.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335154.2:132..2909"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_034191046             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191046
VERSION     XP_034191046.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335155.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191046.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_034335155.2:305..3082"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_076548156             925 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548156
VERSION     XP_076548156.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692041.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..925
                     /product="spire type actin nucleation factor isoform X1"
                     /calculated_mol_wt=104802
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..317
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(299..300,302..304,306..308,313..316)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          335..>357
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(340..341,343..345,347..349,355..357)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          366..390
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(369..370,372..374,376..378,383..386)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          713..747
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..925
                     /gene="spir"
                     /coded_by="XM_076692041.1:30..2807"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwtdir iwraiqarfw
      301 vqviqelrkg vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps
      361 ptphlrkdah avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr
      421 yvpknertsp qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae
      481 dedddedynd elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw
      541 rktgglrltr neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs
      601 rpqsrqhigv testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw
      661 qddkgqkptl gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc
      721 lktrfgllgp wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt
      781 sksswlrgng aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme
      841 nqekrsykgs gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks
      901 srttratirn nvisrltlnl spayv
//
LOCUS       XP_076548157             915 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548157
VERSION     XP_076548157.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692042.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..915
                     /product="spire type actin nucleation factor isoform X2"
                     /calculated_mol_wt=103549
     Region          48..248
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          277..307
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(289..290,292..294,296..298,303..306)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          325..>347
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(330..331,333..335,337..339,345..347)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          356..380
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(359..360,362..364,366..368,373..376)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          703..737
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..915
                     /gene="spir"
                     /coded_by="XM_076692042.1:279..3026"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mhlctrhmqt siesaeahyk avirafvtea
      241 lelsqfldtv aaeklqasqr eiagsdnnkq nsmsvqnldt ldfidwarfw vqviqelrkg
      301 vklkkveanl gsskntsrgr gkgaefeltp yeilmddire rryrlrktps ptphlrkdah
      361 avilefirsr pplkkaserq lpplqkewtl rellmesikk ppnlrssrnr yvpknertsp
      421 qsdyiqstga kenesptgpk pprrevdhsn sstsrrkiip vdfdlkldae dedddedynd
      481 elavtrfeee detshywqlk edysfvdrgs arhrhkdhrd ddeagsanpw rktgglrltr
      541 neyhrfcdaq lesydlatqc psrrasarkh aarrsialta ltgttslphs rpqsrqhigv
      601 testvsqgps pnislnpsps lspqprarqp rrsqtiaegt kdsedqnedw qddkgqkptl
      661 gdmflderls ltleeivhir svltkaeles lpvegrvked vekrrvcflc lktrfgllgp
      721 wgqrcrlcer tvcvkcyskm riptehfahv pvvllspgll lspsssepdt sksswlrgng
      781 aagsapaspa srrkdsslks atpsstpttt pvliltptst ppsharreme nqekrsykgs
      841 gspanvsspk afsgfthqtt eqrlaaerlr gvsmvvchdc rimviqiiks srttratirn
      901 nvisrltlnl spayv
//
LOCUS       XP_076548158             841 aa            linear   INV 03-SEP-2025
DEFINITION  spire type actin nucleation factor isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548158
VERSION     XP_076548158.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692043.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..841
                     /product="spire type actin nucleation factor isoform X3"
                     /calculated_mol_wt=95236
     Region          48..211
                     /region_name="KIND"
                     /note="Kinase non-catalytic C-lobe domain; pfam16474"
                     /db_xref="CDD:465129"
     Region          206..233
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(215..216,218..220,222..224,229..232)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          251..>273
                     /region_name="WH2"
                     /note="Wiskott-Aldrich Syndrome Homology (WASP) region 2
                     (WH2 motif), and similar proteins; cl41728"
                     /db_xref="CDD:425359"
     Site            order(256..257,259..261,263..265,271..273)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409196"
     Region          282..306
                     /region_name="WH2_DmSpire_r3-like"
                     /note="third tandem Wiskott-Aldrich Syndrome Homology
                     (WASP) region 2 (WH2 motif) repeat found in Drosophila
                     melanogaster Spire, and similar proteins; cd22068"
                     /db_xref="CDD:409211"
     Site            order(285..286,288..290,292..294,299..302)
                     /site_type="other"
                     /note="actin-binding motif [polypeptide binding]"
                     /db_xref="CDD:409211"
     Region          629..663
                     /region_name="FYVE_SPIR"
                     /note="FYVE-related domain found in Spir proteins, Spire1
                     and Spire2; cd15748"
                     /db_xref="CDD:277287"
     CDS             1..841
                     /gene="spir"
                     /coded_by="XM_076692043.1:282..2807"
                     /db_xref="GeneID:117609167"
ORIGIN      
        1 mttnkeists ykdslavsdd ekktitsekk eekstnrpkc rldangrlnl kdilisfngp
       61 vsqeqawalc yqtaksfsrl pdnnfyelle lsqivlhkdg dvwlgnlies rqprvteqka
      121 mfslgmmifk aldfglddqe erplspdlea litlmtrpdq ecgeteerpq etddegierd
      181 ssdadvdeyl sqksndtyee rplmyslknv mharfwvqvi qelrkgvklk kveanlgssk
      241 ntsrgrgkga efeltpyeil mddirerryr lrktpsptph lrkdahavil efirsrpplk
      301 kaserqlppl qkewtlrell mesikkppnl rssrnryvpk nertspqsdy iqstgakene
      361 sptgpkpprr evdhsnssts rrkiipvdfd lkldaededd dedyndelav trfeeedets
      421 hywqlkedys fvdrgsarhr hkdhrdddea gsanpwrktg glrltrneyh rfcdaqlesy
      481 dlatqcpsrr asarkhaarr sialtaltgt tslphsrpqs rqhigvtest vsqgpspnis
      541 lnpspslspq prarqprrsq tiaegtkdse dqnedwqddk gqkptlgdmf lderlsltle
      601 eivhirsvlt kaeleslpve grvkedvekr rvcflclktr fgllgpwgqr crlcertvcv
      661 kcyskmript ehfahvpvvl lspglllsps ssepdtskss wlrgngaags apaspasrrk
      721 dsslksatps stptttpvli ltptstppsh arremenqek rsykgsgspa nvsspkafsg
      781 fthqtteqrl aaerlrgvsm vvchdcrimv iqiikssrtt ratirnnvis rltlnlspay
      841 v
//
LOCUS       XP_034191048             657 aa            linear   INV 03-SEP-2025
DEFINITION  fidgetin-like protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034191048
VERSION     XP_034191048.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335157.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191048.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..657
                     /product="fidgetin-like protein 1"
                     /calculated_mol_wt=74906
     Region          357..542
                     /region_name="RecA-like_Figl-1"
                     /note="ATPase domain of Fidgetin-Like 1 (FIGL-1); cd19525"
                     /db_xref="CDD:410933"
     Site            order(380..383,420..426)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:410933"
     Region          567..623
                     /region_name="AAA_lid_3"
                     /note="AAA+ lid domain; pfam17862"
                     /db_xref="CDD:465537"
     Region          <616..648
                     /region_name="Vps4_C"
                     /note="Vps4 C terminal oligomerization domain; pfam09336"
                     /db_xref="CDD:462762"
     CDS             1..657
                     /gene="LOC117609168"
                     /coded_by="XM_034335157.2:1..1974"
                     /db_xref="GeneID:117609168"
ORIGIN      
        1 mditnaknnr eknnflaayh ilkfsqngkd dteivdierr clamkyltak qvesedmaac
       61 llrrsledyq slmenkngvn nywkqfkvqt akinnnpqew ksglsdpdia lnfvkplpcq
      121 dnstmpcqgr lfndrhieni intwknkeml vrnrkisgqv ksnfnqgscn lnsqkkaedn
      181 icseinrsms wpsnskrens svqknytkrs vsksyfvnqe nqnlaqqksv kvepkmhskf
      241 vredssntyk hnhepssmyr nntrlksqaf nqnkedtdma kpklnyfkta rdelntqqmk
      301 ankpaqkktl ggkgsvnsrf vcplkrdkek aegqdnkyyn etdgtaeved erlkniepkl
      361 velikneimd sktiiswddi agleyakkii kevvvypmlr pdiftglrrp pkgillfgpp
      421 gtgktligkc iasqskstff sisassltsk wigegekmvr alftvarvyq psvifvdeid
      481 slltqrsese hessrrlkte flvqldgaat gdedriliig atnrphelde aarrrlvkrl
      541 yvplpefqar rqiinnllit iphnlneedv nniaeqskgy sgadmsnlck easmgpirsi
      601 pfselenirk edvrqvtvdd fkealihvrp svsqsslsty vewdatygtg taqnyka
//
LOCUS       XP_034191049             514 aa            linear   INV 03-SEP-2025
DEFINITION  aurora kinase A activator-like protein bora [Osmia lignaria
            lignaria].
ACCESSION   XP_034191049
VERSION     XP_034191049.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335158.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="aurora kinase A activator-like protein bora"
                     /calculated_mol_wt=58115
     Region          56..252
                     /region_name="BORA_N"
                     /note="Protein aurora borealis N-terminus; pfam15280"
                     /db_xref="CDD:464610"
     CDS             1..514
                     /gene="bora"
                     /coded_by="XM_034335158.2:265..1809"
                     /db_xref="GeneID:117609169"
ORIGIN      
        1 meqlkwttpi ksqrehdnlt tkspvmyktp vkqyeatckh tayqnnmgci svlpnhitpp
       61 sgltkfiarn pfesdltnrl hlsmmsptvf skvsgtsqqs pefswsvdel almqpakiee
      121 fptqqiyctd peteikaqaa idqffkkdki ipspweikkk dnrakikidt pvetlsdsns
      181 essnskkdgw cqtiltfpae lpshiaeilk pyfkftqdqn venddanlsn nslrrklffn
      241 qddsledkee tsvysssvkm ngslvlsssp pesgmlihga pvksqnmdyc rdtsqiipni
      301 spiystvnms renlrsrsrs varldftndm sideslqrrg yssshsldes lnkivkqvak
      361 nkvdnllnsd snamsaktdv ctetkasvkn pvqqennfet enskldskhc nalkcltdsy
      421 rmitnescmf qqtntalgis dqhnsvqdtg yqtysmnsit nvtnnyngtp vkqkacwgdr
      481 illtddefrh etniknifcs tpsrsnretd iyfk
//
LOCUS       XP_034191050             514 aa            linear   INV 03-SEP-2025
DEFINITION  aurora kinase A activator-like protein bora [Osmia lignaria
            lignaria].
ACCESSION   XP_034191050
VERSION     XP_034191050.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335159.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..514
                     /product="aurora kinase A activator-like protein bora"
                     /calculated_mol_wt=58115
     Region          56..252
                     /region_name="BORA_N"
                     /note="Protein aurora borealis N-terminus; pfam15280"
                     /db_xref="CDD:464610"
     CDS             1..514
                     /gene="bora"
                     /coded_by="XM_034335159.2:141..1685"
                     /db_xref="GeneID:117609169"
ORIGIN      
        1 meqlkwttpi ksqrehdnlt tkspvmyktp vkqyeatckh tayqnnmgci svlpnhitpp
       61 sgltkfiarn pfesdltnrl hlsmmsptvf skvsgtsqqs pefswsvdel almqpakiee
      121 fptqqiyctd peteikaqaa idqffkkdki ipspweikkk dnrakikidt pvetlsdsns
      181 essnskkdgw cqtiltfpae lpshiaeilk pyfkftqdqn venddanlsn nslrrklffn
      241 qddsledkee tsvysssvkm ngslvlsssp pesgmlihga pvksqnmdyc rdtsqiipni
      301 spiystvnms renlrsrsrs varldftndm sideslqrrg yssshsldes lnkivkqvak
      361 nkvdnllnsd snamsaktdv ctetkasvkn pvqqennfet enskldskhc nalkcltdsy
      421 rmitnescmf qqtntalgis dqhnsvqdtg yqtysmnsit nvtnnyngtp vkqkacwgdr
      481 illtddefrh etniknifcs tpsrsnretd iyfk
//
LOCUS       XP_034191051             472 aa            linear   INV 03-SEP-2025
DEFINITION  synaptic vesicle membrane protein VAT-1 homolog-like isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034191051
VERSION     XP_034191051.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335160.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..472
                     /product="synaptic vesicle membrane protein VAT-1
                     homolog-like isoform X1"
                     /calculated_mol_wt=51333
     Region          67..406
                     /region_name="MDR3"
                     /note="Medium chain dehydrogenases/reductase
                     (MDR)/zinc-dependent alcohol dehydrogenase-like family;
                     cd08275"
                     /db_xref="CDD:176236"
     Site            order(105..107,110,187,191,212..215,217..218,237..238,241,
                     257,279..280,282,301..302,342..344)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176236"
     CDS             1..472
                     /gene="LOC117609171"
                     /coded_by="XM_034335160.2:207..1625"
                     /db_xref="GeneID:117609171"
ORIGIN      
        1 maeeknetsp naegekipqe aaapskqeke epketkeeek kveengdgdk pkengeekpt
       61 pepkdmraiv lngfgglksv kalrkpeptl aegevlirik acglnfqdlm arqgaidspp
      121 ktpfimgsec agdieqvgeg venfkvgdrv valpdhkawa elvavpatsv falpdgmsyl
      181 daaaitmnyt vaymllfela nltpgkslll hsagggvgqa vvqlaktvkd vtifgvcsks
      241 khealkaagt idhllerstd ytnevrkisp egvdivldcl cgeecnkgya llkpmgkyil
      301 ygssnvvtge tksffsaars wwqvdkvspi klfdenktls gfklrhlmyq qgshgfvrka
      361 vervfalwne gkikpvvdst waledvpeam qkmhdrknig kivldpslep kpkpatpakg
      421 kakdkkaasq esqekkassv eseegekkke peltngtsed ksdsdskeke ss
//
LOCUS       XP_034191052             387 aa            linear   INV 03-SEP-2025
DEFINITION  synaptic vesicle membrane protein VAT-1 homolog-like isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034191052
VERSION     XP_034191052.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335161.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..387
                     /product="synaptic vesicle membrane protein VAT-1
                     homolog-like isoform X2"
                     /calculated_mol_wt=42123
     Region          18..321
                     /region_name="MDR3"
                     /note="Medium chain dehydrogenases/reductase
                     (MDR)/zinc-dependent alcohol dehydrogenase-like family;
                     cd08275"
                     /db_xref="CDD:176236"
     Site            order(20..22,25,102,106,127..130,132..133,152..153,156,
                     172,194..195,197,216..217,257..259)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176236"
     CDS             1..387
                     /gene="LOC117609171"
                     /coded_by="XM_034335161.2:105..1268"
                     /db_xref="GeneID:117609171"
ORIGIN      
        1 mssptidrdr hrprcssgln fqdlmarqga idsppktpfi mgsecagdie qvgegvenfk
       61 vgdrvvalpd hkawaelvav patsvfalpd gmsyldaaai tmnytvayml lfelanltpg
      121 kslllhsagg gvgqavvqla ktvkdvtifg vcskskheal kaagtidhll erstdytnev
      181 rkispegvdi vldclcgeec nkgyallkpm gkyilygssn vvtgetksff saarswwqvd
      241 kvspiklfde nktlsgfklr hlmyqqgshg fvrkavervf alwnegkikp vvdstwaled
      301 vpeamqkmhd rknigkivld pslepkpkpa tpakgkakdk kaasqesqek kassveseeg
      361 ekkkepeltn gtsedksdsd skekess
//
LOCUS       XP_034191053             393 aa            linear   INV 03-SEP-2025
DEFINITION  pummelig isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191053
VERSION     XP_034191053.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335162.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="pummelig isoform X1"
                     /calculated_mol_wt=44369
     Region          30..340
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..393
                     /gene="puml"
                     /coded_by="XM_034335162.2:109..1290"
                     /db_xref="GeneID:117609172"
ORIGIN      
        1 madheivssk swfwgwfsws gssstmlrta ekkilsclkt tyrgwyvdig pvvgssdkiw
       61 tislneespk tpivllhglg agvalwclnl dalasqrpvy aidvlgfgrs srpifsneaq
      121 eaenqlvrsi eewrremqle kfvllghsmg gflaasytiq hperikhlil adpwgfperp
      181 ierisrvpmw ikviaymvep lnplwpvrva gpfgqwliek trpdivkkfv pvlkddttii
      241 sqylhqcnaq tpsgesafha mmqhfgwakn pivrridqln pdipitllyg srswvdnssw
      301 etlkqarssc yinvqaitga ghhvyadkse ifnkyvleac nlsdsiphlt alnirsnikp
      361 aneqqidlil tdeevdtkav qeqelnasqs rss
//
LOCUS       XP_034191054             388 aa            linear   INV 03-SEP-2025
DEFINITION  pummelig isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191054
VERSION     XP_034191054.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335163.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..388
                     /product="pummelig isoform X2"
                     /calculated_mol_wt=43981
     Region          25..335
                     /region_name="PLN02894"
                     /note="hydrolase, alpha/beta fold family protein"
                     /db_xref="CDD:215484"
     CDS             1..388
                     /gene="puml"
                     /coded_by="XM_034335163.2:85..1251"
                     /db_xref="GeneID:117609172"
ORIGIN      
        1 mkeffnwfwg wfswsgssst mlrtaekkil sclkttyrgw yvdigpvvgs sdkiwtisln
       61 eespktpivl lhglgagval wclnldalas qrpvyaidvl gfgrssrpif sneaqeaenq
      121 lvrsieewrr emqlekfvll ghsmggflaa sytiqhperi khliladpwg fperpieris
      181 rvpmwikvia ymveplnplw pvrvagpfgq wliektrpdi vkkfvpvlkd dttiisqylh
      241 qcnaqtpsge safhammqhf gwaknpivrr idqlnpdipi tllygsrswv dnsswetlkq
      301 arsscyinvq aitgaghhvy adkseifnky vleacnlsds iphltalnir snikpaneqq
      361 idliltdeev dtkavqeqel nasqsrss
//
LOCUS       XP_034191057             390 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609173 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191057
VERSION     XP_034191057.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335166.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191057.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="uncharacterized protein LOC117609173 isoform X1"
                     /calculated_mol_wt=45241
     Region          4..48
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     CDS             1..390
                     /gene="LOC117609173"
                     /coded_by="XM_034335166.2:168..1340"
                     /db_xref="GeneID:117609173"
ORIGIN      
        1 mmfpveiweq iflyadpvtl tnlrtvckcw kqiidktlkd ndhwykmckk eipedlwttl
       61 cetlcpikfy tefhtnhavw mamykwwmkc knivkcdtki ecfeplpnhn fneyvtcidv
      121 kgsllaigts egfvyfydih hlhtrtncvv dskeylqsih ilrkestvlc vcctvnnhin
      181 cwdvnkmkli nktsgklvct sysyccisir nyiiiegpvl ktgyefgikd ivaisasnnk
      241 vhfyteggyf atltanteqi nytstpihpp nirirryymf qpniviciae ygylgilvqg
      301 kewkmhnifp vlhgiptavl vhghllvlgl dsgnvhmyyi tdfetidfnn inckkltldl
      361 tavisintvv hhgeyliiay krkiytvkll
//
LOCUS       XP_034191058             344 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609173 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191058
VERSION     XP_034191058.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335167.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191058.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..344
                     /product="uncharacterized protein LOC117609173 isoform X2"
                     /calculated_mol_wt=39583
     CDS             1..344
                     /gene="LOC117609173"
                     /coded_by="XM_034335167.2:162..1196"
                     /db_xref="GeneID:117609173"
ORIGIN      
        1 mckkeipedl wttlcetlcp ikfytefhtn havwmamykw wmkcknivkc dtkiecfepl
       61 pnhnfneyvt cidvkgslla igtsegfvyf ydihhlhtrt ncvvdskeyl qsihilrkes
      121 tvlcvcctvn nhincwdvnk mklinktsgk lvctsysycc isirnyiiie gpvlktgyef
      181 gikdivaisa snnkvhfyte ggyfatltan teqinytstp ihppnirirr yymfqpnivi
      241 ciaeygylgi lvqgkewkmh nifpvlhgip tavlvhghll vlgldsgnvh myyitdfeti
      301 dfnninckkl tldltavisi ntvvhhgeyl iiaykrkiyt vkll
//
LOCUS       XP_034191060             367 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609174 [Osmia lignaria lignaria].
ACCESSION   XP_034191060
VERSION     XP_034191060.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335169.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191060.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..367
                     /product="uncharacterized protein LOC117609174"
                     /calculated_mol_wt=42985
     Region          75..179
                     /region_name="RBFA"
                     /note="Ribosome-binding factor A; pfam02033"
                     /db_xref="CDD:460422"
     CDS             1..367
                     /gene="LOC117609174"
                     /coded_by="XM_034335169.2:1..1104"
                     /db_xref="GeneID:117609174"
ORIGIN      
        1 mycfvkmsnv rifvvkqifr qfgtsntnls isrqsryink fikqgqssrr dpldfsnpfv
       61 fgpsktkvsa hvqrrvnvln tvfmkyvtdl lstgeiepel lnrgieisyv ditkdfkhln
      121 ifwidndvnt ksdttellnk cayklrhels qlkvigcvpp vhfvkckgis qlkeveeklk
      181 tldfgedhvp slysnainct visktslsee kevsknense nvdnvfsvtl pkmrhdvfel
      241 dhfrimskik sslnksrdtl rkqqtnvqyp stslseldye knsnmlttad qqefskfliq
      301 rrkerkyknk mkycnetlid nvpeekdddd yddedwsidq dfneddtdnm ndnyyedhid
      361 refkdkh
//
LOCUS       XP_034191062             318 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2 subunit beta isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034191062
VERSION     XP_034191062.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335171.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..318
                     /product="eukaryotic translation initiation factor 2
                     subunit beta isoform X1"
                     /calculated_mol_wt=36096
     Region          184..293
                     /region_name="eIF2B_5"
                     /note="domain present in translation initiation factor
                     eIF2B and eIF5; smart00653"
                     /db_xref="CDD:214764"
     CDS             1..318
                     /gene="eIF2beta"
                     /coded_by="XM_034335171.2:163..1119"
                     /db_xref="GeneID:117609175"
ORIGIN      
        1 mteedsvfdp tlkkkkkkkk ttfdldaafn eggstgdate tsgdkenqep dppvpaedde
       61 tldlenfgrk kkkkkkafnl deldatlpdt kkedvveqqt eevavddtfd ldmdfsktkk
      121 kkkkkkdlde lvaeeerket edkenaedts lwvgsdrdyt ydellvrvfn imreknpdmv
      181 agkkqkfvmr ppqvvrigtk ktsfanftei cktlhrqpkh lldfllaelg tsgsvdgnsq
      241 liikgrfqqk qienvlrryi keyvtchtcr spdtilqkdt rlfflqcetc gsrcsvasik
      301 sgfqavtgkr aairakta
//
LOCUS       XP_034191063             317 aa            linear   INV 03-SEP-2025
DEFINITION  eukaryotic translation initiation factor 2 subunit beta isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034191063
VERSION     XP_034191063.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..317
                     /product="eukaryotic translation initiation factor 2
                     subunit beta isoform X2"
                     /calculated_mol_wt=36025
     Region          183..292
                     /region_name="eIF2B_5"
                     /note="domain present in translation initiation factor
                     eIF2B and eIF5; smart00653"
                     /db_xref="CDD:214764"
     CDS             1..317
                     /gene="eIF2beta"
                     /coded_by="XM_034335172.2:164..1117"
                     /db_xref="GeneID:117609175"
ORIGIN      
        1 mteedsvfdp tlkkkkkkkk ttfdldaafn eggstgdate tsgdkenqep dppvpaedde
       61 tldlenfgrk kkkkkkafnl deldatlpdt kkedvveqqt eevavddtfd ldmdfsktkk
      121 kkkkkkdlde lvaeeerket edkenedtsl wvgsdrdyty dellvrvfni mreknpdmva
      181 gkkqkfvmrp pqvvrigtkk tsfanfteic ktlhrqpkhl ldfllaelgt sgsvdgnsql
      241 iikgrfqqkq ienvlrryik eyvtchtcrs pdtilqkdtr lfflqcetcg srcsvasiks
      301 gfqavtgkra airakta
//
LOCUS       XP_034191064             230 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609177 [Osmia lignaria lignaria].
ACCESSION   XP_034191064
VERSION     XP_034191064.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335173.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191064.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="uncharacterized protein LOC117609177"
                     /calculated_mol_wt=25972
     CDS             1..230
                     /gene="LOC117609177"
                     /coded_by="XM_034335173.2:161..853"
                     /db_xref="GeneID:117609177"
ORIGIN      
        1 magifnlfge snlnkgnvmt plsrskttig isgasvknag plkpkglsir snsnlnlsas
       61 nkdicnkpqe ylkpkltqln ieghpispgk dcvskktnec pinlspqkvs dkknvqlqkp
      121 sneiifkqps lpkiyakkry pepeslapyc dmqfefddiy tktienefke llmkkkneiv
      181 pyedegfqse peqleykmpi lctspfslde ewrrckspdl pepyfsdmde
//
LOCUS       XP_034190778             519 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609049 [Osmia lignaria lignaria].
ACCESSION   XP_034190778
VERSION     XP_034190778.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334887.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="uncharacterized protein LOC117609049"
                     /calculated_mol_wt=47344
     Region          334..385
                     /region_name="Chitin_bind_4"
                     /note="Insect cuticle protein; pfam00379"
                     /db_xref="CDD:459790"
     CDS             1..519
                     /gene="LOC117609049"
                     /coded_by="XM_034334887.2:171..1730"
                     /db_xref="GeneID:117609049"
ORIGIN      
        1 mskiglavfi valtlvrsep pvnsylppgg ggpgggnggg gggpsntygp pgfdgqngig
       61 ggpgasgggl snsygvptgg ngfngggsgn grpssnggrn ggsrgngfgg gqpsssygpp
      121 ssgfggnggs gggkpsssyg apgggngfgn ggnnggskng fggspsssyg ppesgngfgg
      181 gngggapsgl ygppgrngng nggnggnggn ggrpsssygt pdrnagrpsg lygppgrngn
      241 ngggnggyng gnggsggfgp sggnggypsg gpggngggsg gfpsgspggn ggypsggpag
      301 nggypsggpg gngggngggy genaddgsne pakyefsyev kddqsgadyg htesrdgdra
      361 qgefnvllpd grkqiveyea dqdgfkpqir yegeansqgy gsggpggngg gdfgsngyps
      421 ggpngggagg ngypsgpngn glggngypsg gpnggapggn gypsgrpgsg pdfndggfps
      481 grpgganggy rngnnggngn ggypsgsggd aganggyqy
//
LOCUS       XP_034190756             532 aa            linear   INV 03-SEP-2025
DEFINITION  peroxisomal N(1)-acetyl-spermine/spermidine oxidase [Osmia lignaria
            lignaria].
ACCESSION   XP_034190756
VERSION     XP_034190756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..532
                     /product="peroxisomal N(1)-acetyl-spermine/spermidine
                     oxidase"
                     /calculated_mol_wt=58250
     Region          58..515
                     /region_name="PLN02568"
                     /note="polyamine oxidase"
                     /db_xref="CDD:215308"
     CDS             1..532
                     /gene="LOC117609028"
                     /coded_by="XM_034334865.2:162..1760"
                     /db_xref="GeneID:117609028"
ORIGIN      
        1 mlltrsrlsf rrfqtilvkt ysskkggggk eipsctkvar fescqldpcm ldpckpeptv
       61 viigagmagl saahrlaqcg lqnftileat drpggrihsc wlgdvvaemg atwieggcva
      121 npvftlaaqe gllkpplfrp dpsrglfcts dgraidlpvs itayhtfrqi eqqaaalfsl
      181 gcgrthgtll nfmgvriqqe lhnfpeeqry daarvmygmt ncvrcrcgdd lslvsadqfg
      241 syieipggnv rvplgyvgvl apllrdlpsc slkyckpvsc vrwgaisdsc pravvkccdg
      301 eefpadyviv tvslgvlkhq hdklfcpalp aekveaickl gygyvnkifl eyarpfwvwk
      361 eggiklawsa deladrcdwv kgisnieels tsqhvlcawi cgreaadmel csdeevvesi
      421 trvlrqftgd ptlpypanll rskwcmdqyf agaysymgmd stvghqcdla splpgtcepi
      481 ppillfagea tipghystvh garlsgirea eriiqltkrf ggppkkiiek ss
//
LOCUS       XP_034186736             471 aa            linear   INV 03-SEP-2025
DEFINITION  UTP3 small subunit processome component Sas10 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186736
VERSION     XP_034186736.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330845.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="UTP3 small subunit processome component Sas10"
                     /calculated_mol_wt=54504
     Region          203..283
                     /region_name="Sas10_Utp3"
                     /note="Sas10/Utp3/C1D family; pfam04000"
                     /db_xref="CDD:461123"
     Region          400..470
                     /region_name="Sas10"
                     /note="Sas10 C-terminal domain; pfam09368"
                     /db_xref="CDD:462775"
     CDS             1..471
                     /gene="Sas10"
                     /coded_by="XM_034330845.2:196..1611"
                     /db_xref="GeneID:117607301"
ORIGIN      
        1 mkirkrtkla emhyepedle dvnmnevtds eeeyseserk llqdvrsrhs sesydsdyev
       61 yklnddngkd qvnsedeaqt dsmesdiegl dndfdlpnek awgkkkkayy stdyvdadfa
      121 sitqkdlaea eleeqearni qkrlaeqldd adfgldfiqs kksdeqeshe dtkqlvksdf
      181 sklskrqkqa lmqkespefv alvndfkdrm gevrdtltpf mrlvnkdilp scpavsfikt
      241 kyhlllnyci nisfylmlka krlpvnshpv ikrlaqyrql lnqlesgqgn lmqevqeilk
      301 aqkqgkplyn ifngaeingn eqktnnsske kskypkksak kiksssvpef eseaeaepef
      361 eieekesvse enlendedma eenqvkdiki idtvidsqkk raityqiakn kgltphrkke
      421 qrnprvkhrn kyrkakirrk gavrevrkev tryageisgi kasvkksikl k
//
LOCUS       XP_034186742             108 aa            linear   INV 03-SEP-2025
DEFINITION  10 kDa heat shock protein, mitochondrial isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186742
VERSION     XP_034186742.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330851.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..108
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..108
                     /product="10 kDa heat shock protein, mitochondrial isoform
                     X1"
                     /calculated_mol_wt=11646
     Region          13..106
                     /region_name="Cpn10"
                     /note="Chaperonin 10 Kd subunit; smart00883"
                     /db_xref="CDD:197951"
     Site            order(13,15,17,20,47..48,69,79,84,86,103..105)
                     /site_type="other"
                     /note="oligomerisation interface [polypeptide binding]"
                     /db_xref="CDD:238197"
     Site            29..42
                     /site_type="active"
                     /note="mobile loop [active]"
                     /db_xref="CDD:238197"
     Site            order(56,67)
                     /site_type="active"
                     /note="roof hairpin [active]"
                     /db_xref="CDD:238197"
     CDS             1..108
                     /gene="LOC117607304"
                     /coded_by="XM_034330851.2:140..466"
                     /db_xref="GeneID:117607304"
ORIGIN      
        1 mqdlkaavsa ikrlvplfdr vliqraeaat ktkggivlpe kaqakvlqgt vvaigpgqrn
       61 ekgehiplti kvgdvvllpe yggtkvelee nkelhlfres dilaklev
//
LOCUS       XP_034186743             104 aa            linear   INV 03-SEP-2025
DEFINITION  10 kDa heat shock protein, mitochondrial isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186743
VERSION     XP_034186743.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330852.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..104
                     /product="10 kDa heat shock protein, mitochondrial isoform
                     X2"
                     /calculated_mol_wt=11161
     Region          9..102
                     /region_name="Cpn10"
                     /note="Chaperonin 10 Kd subunit; smart00883"
                     /db_xref="CDD:197951"
     Site            order(9,11,13,16,43..44,65,75,80,82,99..101)
                     /site_type="other"
                     /note="oligomerisation interface [polypeptide binding]"
                     /db_xref="CDD:238197"
     Site            25..38
                     /site_type="active"
                     /note="mobile loop [active]"
                     /db_xref="CDD:238197"
     Site            order(52,63)
                     /site_type="active"
                     /note="roof hairpin [active]"
                     /db_xref="CDD:238197"
     CDS             1..104
                     /gene="LOC117607304"
                     /coded_by="XM_034330852.2:159..473"
                     /db_xref="GeneID:117607304"
ORIGIN      
        1 maavsaikrl vplfdrvliq raeaatktkg givlpekaqa kvlqgtvvai gpgqrnekge
       61 hipltikvgd vvllpeyggt kveleenkel hlfresdila klev
//
LOCUS       XP_034182925             713 aa            linear   INV 03-SEP-2025
DEFINITION  cleavage stimulation factor subunit su(f) [Osmia lignaria
            lignaria].
ACCESSION   XP_034182925
VERSION     XP_034182925.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327034.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..713
                     /product="cleavage stimulation factor subunit su(f)"
                     /calculated_mol_wt=82041
     Region          15..>504
                     /region_name="RNA14"
                     /note="Pre-mRNA 3'-end processing (cleavage and
                     polyadenylation) factor [RNA processing and modification];
                     COG5107"
                     /db_xref="CDD:227438"
     Region          372..640
                     /region_name="Suf"
                     /note="Suppressor of forked protein (Suf); pfam05843"
                     /db_xref="CDD:428647"
     CDS             1..713
                     /gene="su(f)"
                     /coded_by="XM_034327034.2:119..2260"
                     /db_xref="GeneID:117605561"
ORIGIN      
        1 mtddktefdw gneklqraqk tvdespydle awsilireaq nrpitevrpv feklvsvfps
       61 agrywkiyie qemkmrnfek veklfqrclm kilnielwkl ylsyvketka slatykekma
      121 qaydfaldki gmdihsysiw ndyvmflksv eavgsyaenq kisavrkvyq rgvvnpminm
      181 eqlwkdymaf eqninpiiae kmaiersrdy mnarrvakel eavtrglnrs apsvpptghp
      241 eevkqvelwk kyiawersnp lrtedtslva rrvmfaieqc llclghhpav whqaahflel
      301 sskiltekgd vnaaknlsde aatmferats tllsknmlly fahadfeegr vkyekvhqiy
      361 qkfldipdid ptlayvqymk farraegiks artvfkrare dprckhhvyv aaalmeyyct
      421 kdkniafrif elglkkfgdn pdyilcyidy lshlnednnt rvlfervlss gslepeksvd
      481 iwnrflefes nigdlasivk vekrrsavle kikefegket aqlvdrykfl dlypctpmel
      541 rsigymevss varstvgalp ripdpeeaia slprpdlsqm ipykpkvnpl pgehpvpggt
      601 fplppaaaql ctmlpppgcf rgpfvavdll mdvfnriqlp ehaplpvadn gcdtklfdla
      661 ksvhwivdes ndgisigskr rrtrlggdds eeedlppppa ndiyrqrqqk rvk
//
LOCUS       XP_034186697             230 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607275 [Osmia lignaria lignaria].
ACCESSION   XP_034186697
VERSION     XP_034186697.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330806.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="uncharacterized protein LOC117607275"
                     /calculated_mol_wt=24864
     Region          <44..>139
                     /region_name="ser_rich_anae_1"
                     /note="serine-rich protein; NF033849"
                     /db_xref="CDD:468206"
     Region          179..228
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(188,192,203,207)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..230
                     /gene="LOC117607275"
                     /coded_by="XM_034330806.2:106..798"
                     /db_xref="GeneID:117607275"
ORIGIN      
        1 msrhllvsfa ilaifcamtl ssnldghdgk isrvevvgid skdhgmnhgt ghgmdhgigh
       61 gmdlgmdhgi ghgmdhgmdh gighgmdhgm dhgighgmdh gighgmdhgl ghqldghvdh
      121 lslghdqhip lghgmpldhg iplghdqhei qivkipeihe heqpilshds hhdhgnhhcp
      181 knemwskcga hcepscknrn phcskmctag cvcrkglvrd ahhhcvkkch
//
LOCUS       XP_034186700              79 aa            linear   INV 03-SEP-2025
DEFINITION  chymotrypsin inhibitor-like [Osmia lignaria lignaria].
ACCESSION   XP_034186700
VERSION     XP_034186700.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330809.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..79
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..79
                     /product="chymotrypsin inhibitor-like"
                     /calculated_mol_wt=8473
     Region          27..79
                     /region_name="TIL"
                     /note="trypsin inhibitor-like cysteine rich domain;
                     cd19941"
                     /db_xref="CDD:410995"
     Site            order(36,40,51,55)
                     /site_type="other"
                     /note="reactive site [polypeptide binding]"
                     /db_xref="CDD:410995"
     CDS             1..79
                     /gene="LOC117607277"
                     /coded_by="XM_034330809.2:83..322"
                     /db_xref="GeneID:117607277"
ORIGIN      
        1 mmsrlifalf ailvifsasq ssadakcpen evwnscgsac artckqpnpi ctlqcvsrcq
       61 ckgdlvrnnd gkciplkdc
//
LOCUS       XP_034191068             279 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 [Osmia lignaria lignaria].
ACCESSION   XP_034191068
VERSION     XP_034191068.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335177.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..279
                     /product="very long chain fatty acid elongase 7"
                     /calculated_mol_wt=32585
     Region          28..264
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..279
                     /gene="LOC117609180"
                     /coded_by="XM_034335177.2:383..1222"
                     /db_xref="GeneID:117609180"
ORIGIN      
        1 matlirriyh gyryineela dprtqdyfli gspwacigiv gfylyfvhdl gpnlmakrkp
       61 fnldrivqiy naiqivlcty vfykalelaw lfhykfacep vdysyspral eisrtvwlyf
      121 mvklfdlldt vffvlrkkqn qvsflhvyhh agmalgtwaa tkflagghit flgtinsfvh
      181 ivmythylvt slrlskpwwk kyitqiqltq fclillhfvl lawsedcgfp kwtaavmipq
      241 nvfmivlfad fyykayirkp kvatngvtse isngklknq
//
LOCUS       XP_034182898            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182898
VERSION     XP_034182898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327007.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327007.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182899            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182899
VERSION     XP_034182899.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327008.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327008.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182900            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182900
VERSION     XP_034182900.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327009.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327009.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182903            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182903
VERSION     XP_034182903.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327012.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327012.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182904            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182904
VERSION     XP_034182904.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327013.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327013.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182901            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182901
VERSION     XP_034182901.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327010.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327010.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182906            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182906
VERSION     XP_034182906.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327015.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327015.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182905            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182905
VERSION     XP_034182905.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327014.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327014.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182907            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182907
VERSION     XP_034182907.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327016.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327016.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182911            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182911
VERSION     XP_034182911.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327020.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327020.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182910            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182910
VERSION     XP_034182910.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327019.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327019.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182915            2595 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182915
VERSION     XP_034182915.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327024.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2595
                     /product="uncharacterized protein LOC117605558 isoform X1"
                     /calculated_mol_wt=296651
     CDS             1..2595
                     /gene="LOC117605558"
                     /coded_by="XM_034327024.2:672..8459"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mvegaetrgk svfeidrark llmkeklkql aecdevvsse tvnaigrvtd vvraaddnap
       61 ssdefdeile eqhqdlnrfl hlpndtyslt eirpelptcv kygvraglth inlpkfspls
      121 kghqggatai aafatttiyk srcwneavid qiiedgdtfy cesykdintd drrllsildl
      181 krtlcvqnkl tvnvsiedaa yagtfrssep tgmhlvkald lffkrhnagi lsssvlnvai
      241 wkdtkyynif dgqprtdnce paadgvsgta klflvkdlig vlfiileksk vknepfvlys
      301 ieisgvdhyt qpvdtdkpdk pdtkpqrrps gykvqetyra vvqgsyhmth paipqllrsr
      361 tyliialaal vysrlvnank wtsalidlif nqsniyfvdl vrvldkdlnd nefelrlddi
      421 mgdiilgays akvkirtnvv pgygqkkgkm sidvglreff esqtcgllem kgffyaiwrh
      481 ddtyyfmdpf acddegfrsg taeldghmke geaacvtmns sinqmmetii entgsrdrdp
      541 fvihglrvly vktgttpggp lekviyrekg tnrrpqpppt apqtdtklde eidmkprirp
      601 smdkmrdlqv qcpdlmrdae lymmtenepr tytivapepp kepeeeldee eeeeeeeter
      661 edeglteded eerpeeevpe pvpipvrgyr linpnrlvlq gskncldesf dlvsrgkqgl
      721 italtaiays klkeptkwrn mdidqvievg hktygevvdw irkgrpeake keiegegeee
      781 eeedledege qeeqllpips hmdmtmlpvr lklgendvfy ktkmkiiqge anplanlaea
      841 lecyfnrypq lvlenkrlmy giwkegttff lfnpygsdee gwrlrdhpas favvgtlnel
      901 idllygimef ndpsfiihfv gldaiqpgly aseepielpe estieqfktk flpitdedls
      961 kleaeleepe vetqgadtie mdeeeeeeee eedegrrrkf vvaetekpii dpllevkeqe
     1021 qpdapyrlnl illtsnmkif eeseedlkkv heqialeklk ydhpppyvmp snktliklle
     1081 akrakrsvps lvsrfsidsr ldvkkkggms ilmsrsttmm sfaainddvr pskmiklspk
     1141 rylysrilpi clmplraind myiqdqdree amkeeleken edkrddfvge dipdvpvgvr
     1201 irpqliplgp tiktpnlekr titclekkkr kcllakgdeg dgliekilcn tedlllelff
     1261 pgfkakdqpp vddktdvisk tskktlaast ttirkstast qkkgkrmtda trleviedgi
     1321 rilqgnmcle nraanetchf kscffsallc vlakikldpd hfrpstldql iflgdkiyqr
     1381 tgrlrykpyr wfqhveildm iynaitkqsv yadpencqed elsfmleyyl ekdktgilvf
     1441 sncsyafwta neryylfdpy acdekgnase egfcclmefc dlaamlekie rnigkninkp
     1501 yriytvcigh meskkrkkkr kkkivrcvek kteqevraee ppevelstes evslieqtdw
     1561 vktesktslv ydmtivgfsp ikhynasmld vivleneitt pslapfkkss lrltkdmdef
     1621 eavkllvrrk vydrkfkphs avttpldlcv iawslihdpi twsvrtvrgl feasidytfd
     1681 silatedstv semtdgllpe feianyvfrv vfvplhygtl ystegwnlsm slqkifetpg
     1741 ytgvvivcgd ahvgvtkkdd ncfawwtirk tktlrfvtst dmkeflkliv keinepnett
     1801 fvmrvitvsy ahkvdpdcsd tkglhepvvp stslpevhrm paepydleai frptvpdskp
     1861 ifilgsvals nrdavtepkv krcyfvavla vmvkrdivqs pmpgmidkil evaesvyref
     1921 sepkfhiehi lrnvtvmnri fdfrdcassl itltfnprtl rtdfyvqvrk hlrryfksys
     1981 sgiihftncc ygfwysratn ayyyvdpyqc nekgrkvssg gkaclcifst vcqmvknmcl
     2041 nqfeettgff ihqlhvdsvn vppfktfked pmwlyldyhw nfrhapnivk shtkkrkkea
     2101 eegdetvkqf wnnyavevsn liysawgtig sydsrfgdra gknqaaicva vlamqylshp
     2161 srwgpailds avicgdsyyt eslkssmkky tkhfnrfnlq tcfkifphsw tiqfresicg
     2221 ilygtknrmt latalvlafq espnvliecn kitlaalaak dgyyvadpcw vgpplfnkdh
     2281 gaiyvlrcrn mnslvyavvk mlntnqrlnf rltpvvftfe qedfnfmdnk treskrkvll
     2341 epvritpgkv deagppipga httpegdsyl synknftlgk lkshelenpe wpsvepvlee
     2401 enrtsmhvst twhinfgqaa pldratpvfd sqafqhvpee cenrvvdsye plrpvqrsvt
     2461 dfltacndyp rvldfagnrv vtqgpplhct aarsflteta reefrsytad maknvyktyk
     2521 hrlpkrsvhs vtksvsqkig ggeypsatgi htiteeeaes sirdspdtgr eteyetmtea
     2581 dddaedeaet taded
//
LOCUS       XP_034182917            2472 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182917
VERSION     XP_034182917.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327026.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2472
                     /product="uncharacterized protein LOC117605558 isoform X2"
                     /calculated_mol_wt=282852
     CDS             1..2472
                     /gene="LOC117605558"
                     /coded_by="XM_034327026.2:132..7550"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mggataiaaf atttiyksrc wneavidqii edgdtfyces ykdintddrr llsildlkrt
       61 lcvqnkltvn vsiedaayag tfrsseptgm hlvkaldlff krhnagilss svlnvaiwkd
      121 tkyynifdgq prtdncepaa dgvsgtaklf lvkdligvlf iilekskvkn epfvlysiei
      181 sgvdhytqpv dtdkpdkpdt kpqrrpsgyk vqetyravvq gsyhmthpai pqllrsrtyl
      241 iialaalvys rlvnankwts alidlifnqs niyfvdlvrv ldkdlndnef elrlddimgd
      301 iilgaysakv kirtnvvpgy gqkkgkmsid vglreffesq tcgllemkgf fyaiwrhddt
      361 yyfmdpfacd degfrsgtae ldghmkegea acvtmnssin qmmetiient gsrdrdpfvi
      421 hglrvlyvkt gttpggplek viyrekgtnr rpqppptapq tdtkldeeid mkprirpsmd
      481 kmrdlqvqcp dlmrdaelym mteneprtyt ivapeppkep eeeldeeeee eeeeterede
      541 gltededeer peeevpepvp ipvrgyrlin pnrlvlqgsk ncldesfdlv srgkqglita
      601 ltaiaysklk eptkwrnmdi dqvievghkt ygevvdwirk grpeakekei egegeeeeee
      661 dledegeqee qllpipshmd mtmlpvrlkl gendvfyktk mkiiqgeanp lanlaealec
      721 yfnrypqlvl enkrlmygiw kegttfflfn pygsdeegwr lrdhpasfav vgtlnelidl
      781 lygimefndp sfiihfvgld aiqpglyase epielpeest ieqfktkflp itdedlskle
      841 aeleepevet qgadtiemde eeeeeeeeed egrrrkfvva etekpiidpl levkeqeqpd
      901 apyrlnlill tsnmkifees eedlkkvheq ialeklkydh pppyvmpsnk tliklleakr
      961 akrsvpslvs rfsidsrldv kkkggmsilm srsttmmsfa ainddvrpsk miklspkryl
     1021 ysrilpiclm plraindmyi qdqdreeamk eelekenedk rddfvgedip dvpvgvrirp
     1081 qliplgptik tpnlekrtit clekkkrkcl lakgdegdgl iekilcnted lllelffpgf
     1141 kakdqppvdd ktdvisktsk ktlaasttti rkstastqkk gkrmtdatrl eviedgiril
     1201 qgnmclenra anetchfksc ffsallcvla kikldpdhfr pstldqlifl gdkiyqrtgr
     1261 lrykpyrwfq hveildmiyn aitkqsvyad pencqedels fmleyylekd ktgilvfsnc
     1321 syafwtaner yylfdpyacd ekgnaseegf cclmefcdla amlekierni gkninkpyri
     1381 ytvcighmes kkrkkkrkkk ivrcvekkte qevraeeppe velstesevs lieqtdwvkt
     1441 esktslvydm tivgfspikh ynasmldviv leneittpsl apfkksslrl tkdmdefeav
     1501 kllvrrkvyd rkfkphsavt tpldlcviaw slihdpitws vrtvrglfea sidytfdsil
     1561 atedstvsem tdgllpefei anyvfrvvfv plhygtlyst egwnlsmslq kifetpgytg
     1621 vvivcgdahv gvtkkddncf awwtirktkt lrfvtstdmk eflklivkei nepnettfvm
     1681 rvitvsyahk vdpdcsdtkg lhepvvpsts lpevhrmpae pydleaifrp tvpdskpifi
     1741 lgsvalsnrd avtepkvkrc yfvavlavmv krdivqspmp gmidkileva esvyrefsep
     1801 kfhiehilrn vtvmnrifdf rdcasslitl tfnprtlrtd fyvqvrkhlr ryfksyssgi
     1861 ihftnccygf wysratnayy yvdpyqcnek grkvssggka clcifstvcq mvknmclnqf
     1921 eettgffihq lhvdsvnvpp fktfkedpmw lyldyhwnfr hapnivksht kkrkkeaeeg
     1981 detvkqfwnn yavevsnliy sawgtigsyd srfgdragkn qaaicvavla mqylshpsrw
     2041 gpaildsavi cgdsyytesl kssmkkytkh fnrfnlqtcf kifphswtiq fresicgily
     2101 gtknrmtlat alvlafqesp nvliecnkit laalaakdgy yvadpcwvgp plfnkdhgai
     2161 yvlrcrnmns lvyavvkmln tnqrlnfrlt pvvftfeqed fnfmdnktre skrkvllepv
     2221 ritpgkvdea gppipgahtt pegdsylsyn knftlgklks helenpewps vepvleeenr
     2281 tsmhvsttwh infgqaapld ratpvfdsqa fqhvpeecen rvvdsyeplr pvqrsvtdfl
     2341 tacndyprvl dfagnrvvtq gpplhctaar sfltetaree frsytadmak nvyktykhrl
     2401 pkrsvhsvtk svsqkiggge ypsatgihti teeeaessir dspdtgrete yetmteaddd
     2461 aedeaettad ed
//
LOCUS       XP_034182918            2383 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605558 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182918
VERSION     XP_034182918.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327027.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2383
                     /product="uncharacterized protein LOC117605558 isoform X3"
                     /calculated_mol_wt=273022
     CDS             1..2383
                     /gene="LOC117605558"
                     /coded_by="XM_034327027.2:492..7643"
                     /db_xref="GeneID:117605558"
ORIGIN      
        1 mhlvkaldlf fkrhnagils ssvlnvaiwk dtkyynifdg qprtdncepa adgvsgtakl
       61 flvkdligvl fiilekskvk nepfvlysie isgvdhytqp vdtdkpdkpd tkpqrrpsgy
      121 kvqetyravv qgsyhmthpa ipqllrsrty liialaalvy srlvnankwt salidlifnq
      181 sniyfvdlvr vldkdlndne felrlddimg diilgaysak vkirtnvvpg ygqkkgkmsi
      241 dvglreffes qtcgllemkg ffyaiwrhdd tyyfmdpfac ddegfrsgta eldghmkege
      301 aacvtmnssi nqmmetiien tgsrdrdpfv ihglrvlyvk tgttpggple kviyrekgtn
      361 rrpqppptap qtdtkldeei dmkprirpsm dkmrdlqvqc pdlmrdaely mmteneprty
      421 tivapeppke peeeldeeee eeeeetered egltededee rpeeevpepv pipvrgyrli
      481 npnrlvlqgs kncldesfdl vsrgkqglit altaiayskl keptkwrnmd idqvievghk
      541 tygevvdwir kgrpeakeke iegegeeeee edledegeqe eqllpipshm dmtmlpvrlk
      601 lgendvfykt kmkiiqgean planlaeale cyfnrypqlv lenkrlmygi wkegttfflf
      661 npygsdeegw rlrdhpasfa vvgtlnelid llygimefnd psfiihfvgl daiqpglyas
      721 eepielpees tieqfktkfl pitdedlskl eaeleepeve tqgadtiemd eeeeeeeeee
      781 degrrrkfvv aetekpiidp llevkeqeqp dapyrlnlil ltsnmkifee seedlkkvhe
      841 qialeklkyd hpppyvmpsn ktliklleak rakrsvpslv srfsidsrld vkkkggmsil
      901 msrsttmmsf aainddvrps kmiklspkry lysrilpicl mplraindmy iqdqdreeam
      961 keelekened krddfvgedi pdvpvgvrir pqliplgpti ktpnlekrti tclekkkrkc
     1021 llakgdegdg liekilcnte dlllelffpg fkakdqppvd dktdviskts kktlaasttt
     1081 irkstastqk kgkrmtdatr leviedgiri lqgnmclenr aanetchfks cffsallcvl
     1141 akikldpdhf rpstldqlif lgdkiyqrtg rlrykpyrwf qhveildmiy naitkqsvya
     1201 dpencqedel sfmleyylek dktgilvfsn csyafwtane ryylfdpyac dekgnaseeg
     1261 fcclmefcdl aamlekiern igkninkpyr iytvcighme skkrkkkrkk kivrcvekkt
     1321 eqevraeepp evelstesev slieqtdwvk tesktslvyd mtivgfspik hynasmldvi
     1381 vleneittps lapfkksslr ltkdmdefea vkllvrrkvy drkfkphsav ttpldlcvia
     1441 wslihdpitw svrtvrglfe asidytfdsi latedstvse mtdgllpefe ianyvfrvvf
     1501 vplhygtlys tegwnlsmsl qkifetpgyt gvvivcgdah vgvtkkddnc fawwtirktk
     1561 tlrfvtstdm keflklivke inepnettfv mrvitvsyah kvdpdcsdtk glhepvvpst
     1621 slpevhrmpa epydleaifr ptvpdskpif ilgsvalsnr davtepkvkr cyfvavlavm
     1681 vkrdivqspm pgmidkilev aesvyrefse pkfhiehilr nvtvmnrifd frdcasslit
     1741 ltfnprtlrt dfyvqvrkhl rryfksyssg iihftnccyg fwysratnay yyvdpyqcne
     1801 kgrkvssggk aclcifstvc qmvknmclnq feettgffih qlhvdsvnvp pfktfkedpm
     1861 wlyldyhwnf rhapnivksh tkkrkkeaee gdetvkqfwn nyavevsnli ysawgtigsy
     1921 dsrfgdragk nqaaicvavl amqylshpsr wgpaildsav icgdsyytes lkssmkkytk
     1981 hfnrfnlqtc fkifphswti qfresicgil ygtknrmtla talvlafqes pnvliecnki
     2041 tlaalaakdg yyvadpcwvg pplfnkdhga iyvlrcrnmn slvyavvkml ntnqrlnfrl
     2101 tpvvftfeqe dfnfmdnktr eskrkvllep vritpgkvde agppipgaht tpegdsylsy
     2161 nknftlgklk shelenpewp svepvleeen rtsmhvsttw hinfgqaapl dratpvfdsq
     2221 afqhvpeece nrvvdsyepl rpvqrsvtdf ltacndyprv ldfagnrvvt qgpplhctaa
     2281 rsfltetare efrsytadma knvyktykhr lpkrsvhsvt ksvsqkiggg eypsatgiht
     2341 iteeeaessi rdspdtgret eyetmteadd daedeaetta ded
//
LOCUS       XP_034182919            1513 aa            linear   INV 03-SEP-2025
DEFINITION  rapamycin-insensitive companion of Tor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182919
VERSION     XP_034182919.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327028.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182919.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1513
                     /product="rapamycin-insensitive companion of Tor isoform
                     X1"
                     /calculated_mol_wt=170715
     Region          62..415
                     /region_name="RICTOR_N"
                     /note="Rapamycin-insensitive companion of mTOR, N-term;
                     pfam14664"
                     /db_xref="CDD:464246"
     Region          629..731
                     /region_name="RICTOR_M"
                     /note="Rapamycin-insensitive companion of mTOR, middle
                     domain; pfam14666"
                     /db_xref="CDD:464248"
     Region          740..849
                     /region_name="RasGEF_N_2"
                     /note="Rapamycin-insensitive companion of mTOR RasGEF_N
                     domain; pfam14663"
                     /db_xref="CDD:464245"
     Region          1016..1085
                     /region_name="RICTOR_V"
                     /note="Rapamycin-insensitive companion of mTOR, domain 5;
                     pfam14668"
                     /db_xref="CDD:464249"
     CDS             1..1513
                     /gene="rictor"
                     /coded_by="XM_034327028.2:554..5095"
                     /db_xref="GeneID:117605559"
ORIGIN      
        1 maisswmiwg rslrtsrshr srqdsedscv qldlsrglke nvkevltnlc qrhnvpsikk
       61 laylnaivkl isendspeyg ysindifccl rvglvheatq vraaalravr ymlkkeqdvi
      121 ainklqypyf varsmdvnlr nemermqalr lvrrilvlap kyfspilars lvsltnggie
      181 ekdgafrafl atlcelgiln snlliscggi galaraamtg qsptiiesvv gvllkllntp
      241 etrnsvsllc faapycelhs lsidrtkeek erfaasklal lsilrsysgv lhfcrpddns
      301 glkaiadily veqlevrgav lellyellnl plptwtdepd valaavdpsr ardswklseg
      361 fvaaegkyil pslssrcpni teihlallvy allecglhra laetivstdt fisvraavll
      421 gallhlahsl lpsevcdltp plpnllehas agkhqalaav tilermhtmm rrrpapaslf
      481 ldkllqagtw lrptlprrqr tssrhwlrre spttpllkda qvlsfkdaha wnwpvvqsil
      541 rsredtmril hdsdhrlfmk rlvryfkpcs naysrvelat naslareatl agcdllncll
      601 elhepestkl lneligdvae qissirtaqs aheclfsprh msttccqkyf lflgqlshsa
      661 kgtvilkgfn lleklqdlal atnhdcyvkl ivssldysrd gpnrkvltki iteaaldstr
      721 lyatqflrli lrarmadayr waiallsdrl sdsnktvalt alealheace epeyleamlq
      781 qgsqcrdwek wlnelgdkgy llkirlysih qgfttisspa eelekwispg gfaeryvgli
      841 egeihdsltr rqrdetgsyh rrttnvpmtp hnifilphll gqlaqhdlgm qllmrrnviq
      901 rfariiqrfk mevggrdses nsrciktnrs viddvypmse esgtdeties nkletimdae
      961 tvegidmyst qkvdslidir rktslddsrr ttperswrle tesredqats lnkrilrvks
     1021 alwllghvgt saagveqlnh lgilelitsm aetcsyyavr atslyalsli gttragadal
     1081 liydwpcvrh rrgdhwpvlp ptsrypapsp vpiqrhhrsl sdgkpelpep varrtrnrse
     1141 saatdlearr yallergetp spvssiqrls qqdaegyarl rslqrhrrps yshsslemys
     1201 ldgrlslqsl sefdssrswi aeqvltptpp ppednndnlf ymgialpkrl itifpelpql
     1261 svptitddap ksdiettevd eescsecdvd aehyriclvc yhgksisrds lpeqdiklqr
     1321 kilrhaqrla npiwyrhsrq tllrlrqlhs dkfqdtclfs dvaarlgsgt yrmparrflq
     1381 elfldstfdv lyveaanilk vhednddksg rfpistales dshvpseiki ngrltfseiq
     1441 vtqldalvee agadtcvtqq yseksqefsc kqerrsdeki iaeilkpeek lrlskssdrl
     1501 lkvsgtntai sld
//
LOCUS       XP_076548159            1507 aa            linear   INV 03-SEP-2025
DEFINITION  rapamycin-insensitive companion of Tor isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548159
VERSION     XP_076548159.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692044.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1507
                     /product="rapamycin-insensitive companion of Tor isoform
                     X2"
                     /calculated_mol_wt=170185
     Region          62..415
                     /region_name="RICTOR_N"
                     /note="Rapamycin-insensitive companion of mTOR, N-term;
                     pfam14664"
                     /db_xref="CDD:464246"
     Region          629..731
                     /region_name="RICTOR_M"
                     /note="Rapamycin-insensitive companion of mTOR, middle
                     domain; pfam14666"
                     /db_xref="CDD:464248"
     Region          740..849
                     /region_name="RasGEF_N_2"
                     /note="Rapamycin-insensitive companion of mTOR RasGEF_N
                     domain; pfam14663"
                     /db_xref="CDD:464245"
     Region          1016..1085
                     /region_name="RICTOR_V"
                     /note="Rapamycin-insensitive companion of mTOR, domain 5;
                     pfam14668"
                     /db_xref="CDD:464249"
     CDS             1..1507
                     /gene="rictor"
                     /coded_by="XM_076692044.1:555..5078"
                     /db_xref="GeneID:117605559"
ORIGIN      
        1 maisswmiwg rslrtsrshr srqdsedscv qldlsrglke nvkevltnlc qrhnvpsikk
       61 laylnaivkl isendspeyg ysindifccl rvglvheatq vraaalravr ymlkkeqdvi
      121 ainklqypyf varsmdvnlr nemermqalr lvrrilvlap kyfspilars lvsltnggie
      181 ekdgafrafl atlcelgiln snlliscggi galaraamtg qsptiiesvv gvllkllntp
      241 etrnsvsllc faapycelhs lsidrtkeek erfaasklal lsilrsysgv lhfcrpddns
      301 glkaiadily veqlevrgav lellyellnl plptwtdepd valaavdpsr ardswklseg
      361 fvaaegkyil pslssrcpni teihlallvy allecglhra laetivstdt fisvraavll
      421 gallhlahsl lpsevcdltp plpnllehas agkhqalaav tilermhtmm rrrpapaslf
      481 ldkllqagtw lrptlprrqr tssrhwlrre spttpllkda qvlsfkdaha wnwpvvqsil
      541 rsredtmril hdsdhrlfmk rlvryfkpcs naysrvelat naslareatl agcdllncll
      601 elhepestkl lneligdvae qissirtaqs aheclfsprh msttccqkyf lflgqlshsa
      661 kgtvilkgfn lleklqdlal atnhdcyvkl ivssldysrd gpnrkvltki iteaaldstr
      721 lyatqflrli lrarmadayr waiallsdrl sdsnktvalt alealheace epeyleamlq
      781 qgsqcrdwek wlnelgdkgy llkirlysih qgfttisspa eelekwispg gfaeryvgli
      841 egeihdsltr rqrdetgsyh rrttnvpmtp hnifilphll gqlaqhdlgm qllmrrnviq
      901 rfariiqrfk mevggrdses nsrciktnrs viddvypmse esgtdeties nkletimdae
      961 tvegidmyst qkvdslidir rktslddsrr ttperswrle tesredqats lnkrilrvks
     1021 alwllghvgt saagveqlnh lgilelitsm aetcsyyavr atslyalsli gttragadal
     1081 liydwpcvrh rrgdhwpvlp ptsrypapsp vpiqrhhrsl sdgkpelpep varrtrnrse
     1141 saatdlearr yallergetp spvssiqrls qqdaegyarl rslqrhrrps yshsslemys
     1201 ldgrlslqsl sefdssrswi aeqvltptpp ppednndnlf ymgialpkrl itifpelpql
     1261 svptitddap ksdiettevd eescsecdvd aehyriclvc yhgksisrds lpeqdiklqr
     1321 kilrhaqrla npiwyrhsrq tllrlrqlhs dkfqdtclfs dvaarlgsgt yrmparrflq
     1381 elfldstfdv lyveaanilk vhednddksg rfpistales dshvpseiki ngrltfseiq
     1441 vtqldalvee agadtcvtqq yseksqefsc kqerrsdeki iaeilkpeek lrlskssdrl
     1501 lkvsdkv
//
LOCUS       XP_076548160            1506 aa            linear   INV 03-SEP-2025
DEFINITION  rapamycin-insensitive companion of Tor isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548160
VERSION     XP_076548160.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692045.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1506
                     /product="rapamycin-insensitive companion of Tor isoform
                     X3"
                     /calculated_mol_wt=170028
     Region          62..415
                     /region_name="RICTOR_N"
                     /note="Rapamycin-insensitive companion of mTOR, N-term;
                     pfam14664"
                     /db_xref="CDD:464246"
     Region          629..731
                     /region_name="RICTOR_M"
                     /note="Rapamycin-insensitive companion of mTOR, middle
                     domain; pfam14666"
                     /db_xref="CDD:464248"
     Region          740..849
                     /region_name="RasGEF_N_2"
                     /note="Rapamycin-insensitive companion of mTOR RasGEF_N
                     domain; pfam14663"
                     /db_xref="CDD:464245"
     Region          1016..1085
                     /region_name="RICTOR_V"
                     /note="Rapamycin-insensitive companion of mTOR, domain 5;
                     pfam14668"
                     /db_xref="CDD:464249"
     CDS             1..1506
                     /gene="rictor"
                     /coded_by="XM_076692045.1:555..5075"
                     /db_xref="GeneID:117605559"
ORIGIN      
        1 maisswmiwg rslrtsrshr srqdsedscv qldlsrglke nvkevltnlc qrhnvpsikk
       61 laylnaivkl isendspeyg ysindifccl rvglvheatq vraaalravr ymlkkeqdvi
      121 ainklqypyf varsmdvnlr nemermqalr lvrrilvlap kyfspilars lvsltnggie
      181 ekdgafrafl atlcelgiln snlliscggi galaraamtg qsptiiesvv gvllkllntp
      241 etrnsvsllc faapycelhs lsidrtkeek erfaasklal lsilrsysgv lhfcrpddns
      301 glkaiadily veqlevrgav lellyellnl plptwtdepd valaavdpsr ardswklseg
      361 fvaaegkyil pslssrcpni teihlallvy allecglhra laetivstdt fisvraavll
      421 gallhlahsl lpsevcdltp plpnllehas agkhqalaav tilermhtmm rrrpapaslf
      481 ldkllqagtw lrptlprrqr tssrhwlrre spttpllkda qvlsfkdaha wnwpvvqsil
      541 rsredtmril hdsdhrlfmk rlvryfkpcs naysrvelat naslareatl agcdllncll
      601 elhepestkl lneligdvae qissirtaqs aheclfsprh msttccqkyf lflgqlshsa
      661 kgtvilkgfn lleklqdlal atnhdcyvkl ivssldysrd gpnrkvltki iteaaldstr
      721 lyatqflrli lrarmadayr waiallsdrl sdsnktvalt alealheace epeyleamlq
      781 qgsqcrdwek wlnelgdkgy llkirlysih qgfttisspa eelekwispg gfaeryvgli
      841 egeihdsltr rqrdetgsyh rrttnvpmtp hnifilphll gqlaqhdlgm qllmrrnviq
      901 rfariiqrfk mevggrdses nsrciktnrs viddvypmse esgtdeties nkletimdae
      961 tvegidmyst qkvdslidir rktslddsrr ttperswrle tesredqats lnkrilrvks
     1021 alwllghvgt saagveqlnh lgilelitsm aetcsyyavr atslyalsli gttragadal
     1081 liydwpcvrh rrgdhwpvlp ptsrypapsp vpiqrhhrsl sdgkpelpep varrtrnrse
     1141 saatdlearr yallergetp spvssiqrls qqdaegyarl rslqrhrrps yshsslemys
     1201 ldgrlslqsl sefdssrswi aeqvltptpp ppednndnlf ymgialpkrl itifpelpql
     1261 svptitddap ksdiettevd eescsecdvd aehyriclvc yhgksisrds lpeqdiklqr
     1321 kilrhaqrla npiwyrhsrq tllrlrqlhs dkfqdtclfs dvaarlgsgt yrmparrflq
     1381 elfldstfdv lyveaanilk vhednddksg rfpistales dshvpseiki ngrltfseiq
     1441 vtqldalvee agadtcvtqq yseksqefsc kqerrsdeki iaeilkpeek lrlskssdrl
     1501 lkvsvs
//
LOCUS       XP_076548161            1402 aa            linear   INV 03-SEP-2025
DEFINITION  rapamycin-insensitive companion of Tor isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548161
VERSION     XP_076548161.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692046.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1402
                     /product="rapamycin-insensitive companion of Tor isoform
                     X4"
                     /calculated_mol_wt=158082
     Region          1..304
                     /region_name="RICTOR_N"
                     /note="Rapamycin-insensitive companion of mTOR, N-term;
                     pfam14664"
                     /db_xref="CDD:464246"
     Region          518..620
                     /region_name="RICTOR_M"
                     /note="Rapamycin-insensitive companion of mTOR, middle
                     domain; pfam14666"
                     /db_xref="CDD:464248"
     Region          629..738
                     /region_name="RasGEF_N_2"
                     /note="Rapamycin-insensitive companion of mTOR RasGEF_N
                     domain; pfam14663"
                     /db_xref="CDD:464245"
     Region          905..974
                     /region_name="RICTOR_V"
                     /note="Rapamycin-insensitive companion of mTOR, domain 5;
                     pfam14668"
                     /db_xref="CDD:464249"
     CDS             1..1402
                     /gene="rictor"
                     /coded_by="XM_076692046.1:397..4605"
                     /db_xref="GeneID:117605559"
ORIGIN      
        1 mlkkeqdvia inklqypyfv arsmdvnlrn emermqalrl vrrilvlapk yfspilarsl
       61 vsltnggiee kdgafrafla tlcelgilns nlliscggig alaraamtgq sptiiesvvg
      121 vllkllntpe trnsvsllcf aapycelhsl sidrtkeeke rfaasklall silrsysgvl
      181 hfcrpddnsg lkaiadilyv eqlevrgavl ellyellnlp lptwtdepdv alaavdpsra
      241 rdswklsegf vaaegkyilp slssrcpnit eihlallvya llecglhral aetivstdtf
      301 isvraavllg allhlahsll psevcdltpp lpnllehasa gkhqalaavt ilermhtmmr
      361 rrpapaslfl dkllqagtwl rptlprrqrt ssrhwlrres pttpllkdaq vlsfkdahaw
      421 nwpvvqsilr sredtmrilh dsdhrlfmkr lvryfkpcsn aysrvelatn aslareatla
      481 gcdllnclle lhepestkll neligdvaeq issirtaqsa heclfsprhm sttccqkyfl
      541 flgqlshsak gtvilkgfnl leklqdlala tnhdcyvkli vssldysrdg pnrkvltkii
      601 teaaldstrl yatqflrlil rarmadayrw aiallsdrls dsnktvalta lealheacee
      661 peyleamlqq gsqcrdwekw lnelgdkgyl lkirlysihq gfttisspae elekwispgg
      721 faeryvglie geihdsltrr qrdetgsyhr rttnvpmtph nifilphllg qlaqhdlgmq
      781 llmrrnviqr fariiqrfkm evggrdsesn srciktnrsv iddvypmsee sgtdetiesn
      841 kletimdaet vegidmystq kvdslidirr ktslddsrrt tperswrlet esredqatsl
      901 nkrilrvksa lwllghvgts aagveqlnhl gilelitsma etcsyyavra tslyalslig
      961 ttragadall iydwpcvrhr rgdhwpvlpp tsrypapspv piqrhhrsls dgkpelpepv
     1021 arrtrnrses aatdlearry allergetps pvssiqrlsq qdaegyarlr slqrhrrpsy
     1081 shsslemysl dgrlslqsls efdssrswia eqvltptppp pednndnlfy mgialpkrli
     1141 tifpelpqls vptitddapk sdiettevde escsecdvda ehyriclvcy hgksisrdsl
     1201 peqdiklqrk ilrhaqrlan piwyrhsrqt llrlrqlhsd kfqdtclfsd vaarlgsgty
     1261 rmparrflqe lfldstfdvl yveaanilkv hednddksgr fpistalesd shvpseikin
     1321 grltfseiqv tqldalveea gadtcvtqqy seksqefsck qerrsdekii aeilkpeekl
     1381 rlskssdrll kvsgtntais ld
//
LOCUS       XP_034182920            1395 aa            linear   INV 03-SEP-2025
DEFINITION  rapamycin-insensitive companion of Tor isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182920
VERSION     XP_034182920.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327029.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182920.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1395
                     /product="rapamycin-insensitive companion of Tor isoform
                     X5"
                     /calculated_mol_wt=157637
     Region          62..415
                     /region_name="RICTOR_N"
                     /note="Rapamycin-insensitive companion of mTOR, N-term;
                     pfam14664"
                     /db_xref="CDD:464246"
     Region          629..731
                     /region_name="RICTOR_M"
                     /note="Rapamycin-insensitive companion of mTOR, middle
                     domain; pfam14666"
                     /db_xref="CDD:464248"
     Region          740..849
                     /region_name="RasGEF_N_2"
                     /note="Rapamycin-insensitive companion of mTOR RasGEF_N
                     domain; pfam14663"
                     /db_xref="CDD:464245"
     Region          1016..1085
                     /region_name="RICTOR_V"
                     /note="Rapamycin-insensitive companion of mTOR, domain 5;
                     pfam14668"
                     /db_xref="CDD:464249"
     CDS             1..1395
                     /gene="rictor"
                     /coded_by="XM_034327029.2:557..4744"
                     /db_xref="GeneID:117605559"
ORIGIN      
        1 maisswmiwg rslrtsrshr srqdsedscv qldlsrglke nvkevltnlc qrhnvpsikk
       61 laylnaivkl isendspeyg ysindifccl rvglvheatq vraaalravr ymlkkeqdvi
      121 ainklqypyf varsmdvnlr nemermqalr lvrrilvlap kyfspilars lvsltnggie
      181 ekdgafrafl atlcelgiln snlliscggi galaraamtg qsptiiesvv gvllkllntp
      241 etrnsvsllc faapycelhs lsidrtkeek erfaasklal lsilrsysgv lhfcrpddns
      301 glkaiadily veqlevrgav lellyellnl plptwtdepd valaavdpsr ardswklseg
      361 fvaaegkyil pslssrcpni teihlallvy allecglhra laetivstdt fisvraavll
      421 gallhlahsl lpsevcdltp plpnllehas agkhqalaav tilermhtmm rrrpapaslf
      481 ldkllqagtw lrptlprrqr tssrhwlrre spttpllkda qvlsfkdaha wnwpvvqsil
      541 rsredtmril hdsdhrlfmk rlvryfkpcs naysrvelat naslareatl agcdllncll
      601 elhepestkl lneligdvae qissirtaqs aheclfsprh msttccqkyf lflgqlshsa
      661 kgtvilkgfn lleklqdlal atnhdcyvkl ivssldysrd gpnrkvltki iteaaldstr
      721 lyatqflrli lrarmadayr waiallsdrl sdsnktvalt alealheace epeyleamlq
      781 qgsqcrdwek wlnelgdkgy llkirlysih qgfttisspa eelekwispg gfaeryvgli
      841 egeihdsltr rqrdetgsyh rrttnvpmtp hnifilphll gqlaqhdlgm qllmrrnviq
      901 rfariiqrfk mevggrdses nsrciktnrs viddvypmse esgtdeties nkletimdae
      961 tvegidmyst qkvdslidir rktslddsrr ttperswrle tesredqats lnkrilrvks
     1021 alwllghvgt saagveqlnh lgilelitsm aetcsyyavr atslyalsli gttragadal
     1081 liydwpcvrh rrgdhwpvlp ptsrypapsp vpiqrhhrsl sdgkpelpep varrtrnrse
     1141 saatdlearr yallergetp spvssiqrls qqdaegyarl rslqrhrrps yshsslemys
     1201 ldgrlslqsl sefdssrswi aeqvltptpp ppednndnlf ymgialpkrl itifpelpql
     1261 svptitddap ksdiettevd eescsecdvd aehyriclvc yhgksisrds lpeqdiklqr
     1321 kilrhaqrla npiwyrhsrq tllrlrqlhs dkfqdtclfs dvaarlgsgt yrmparrflq
     1381 elfldstfdv kqlif
//
LOCUS       XP_034182921            1009 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent DNA helicase Q5 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034182921
VERSION     XP_034182921.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327030.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1009
                     /product="ATP-dependent DNA helicase Q5 isoform X1"
                     /calculated_mol_wt=115235
     Region          5..420
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     Region          <745..955
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..1009
                     /gene="LOC117605560"
                     /coded_by="XM_034327030.2:133..3162"
                     /db_xref="GeneID:117605560"
ORIGIN      
        1 mlstvlksvf gyddfksdiq kqattaiykg kqdvfvcmpt gsgkslcfql paimkenkvt
       61 ivfspllalm knqidylvsi kinarslnsh tstkernaim kdllsdspki kllyvtpemg
      121 vqqhfqdmii klkkiktlsy fvideahcls qwghdfrpsy rqlgifkklc pnipivalta
      181 taakevkddi mqslnmknpl ifsvpvfrpn lyydvwfiei ldkpfehlks fiietlgsqs
      241 esiakvqknc giiycrkket ceiianklsk sgistlayha glknqernev qnkwtsgevp
      301 viaatcsfgm gvdkgsvrfv ihwtvpqnia ayyqesgrag rdgkpafcri yfsneeykpi
      361 aflikeeitq knselvklrw nnfekavsyc leakcrhavf skyfgdgppp ckdrcdvckn
      421 kdavqamisq femsqtrlqk snsnldgial skydnledey keesgtrerl iaeskreake
      481 liekqfairr tnskldeikk qnredakqsh vraaestdik ikglavqvre hfyvqlrsal
      541 idnyekmisd ikgqsqekhl hsiaqdleyr vlcntkvank ykfdmsklis svrkctstnt
      601 lheclrdfnv ssnnvttyks kqevsndsle hdtesesvin kfddkdiris fqessiskdi
      661 pcstfktale lrndrnliag tsndsrnylf dasilkddsk dvnaigrknk drnspvkvdi
      721 knseedktie lqqlsgftca rklheennil sknyskksrk slaksvknds ekkkasptkk
      781 siyekilqaa sanlkktven ttnssipeas nnidstslnk deiklnsqtr nkrkninsfk
      841 ssidvesnkk lklnepqvqv lagdterisd hnngvnanet stnetieied rkddikdvqc
      901 qeelsmiktv eeeilrehkn lqrkqdkthr skqqkgedkk vlvpadkatq fktaeilkah
      961 lmkyypskri pdrttfsktc remhhkllak kifdkegirq fvikymaqn
//
LOCUS       XP_034182922             972 aa            linear   INV 03-SEP-2025
DEFINITION  ATP-dependent DNA helicase Q5 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182922
VERSION     XP_034182922.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327031.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..972
                     /product="ATP-dependent DNA helicase Q5 isoform X2"
                     /calculated_mol_wt=111077
     Region          1..383
                     /region_name="RecQ"
                     /note="Superfamily II DNA helicase RecQ [Replication,
                     recombination and repair]; COG0514"
                     /db_xref="CDD:440280"
     Region          <708..918
                     /region_name="PTZ00108"
                     /note="DNA topoisomerase 2-like protein; Provisional"
                     /db_xref="CDD:240271"
     CDS             1..972
                     /gene="LOC117605560"
                     /coded_by="XM_034327031.2:141..3059"
                     /db_xref="GeneID:117605560"
ORIGIN      
        1 mptgsgkslc fqlpaimken kvtivfspll almknqidyl vsikinarsl nshtstkern
       61 aimkdllsds pkikllyvtp emgvqqhfqd miiklkkikt lsyfvideah clsqwghdfr
      121 psyrqlgifk klcpnipiva ltataakevk ddimqslnmk nplifsvpvf rpnlyydvwf
      181 ieildkpfeh lksfiietlg sqsesiakvq kncgiiycrk ketceiiank lsksgistla
      241 yhaglknqer nevqnkwtsg evpviaatcs fgmgvdkgsv rfvihwtvpq niaayyqesg
      301 ragrdgkpaf criyfsneey kpiaflikee itqknselvk lrwnnfekav sycleakcrh
      361 avfskyfgdg pppckdrcdv cknkdavqam isqfemsqtr lqksnsnldg ialskydnle
      421 deykeesgtr erliaeskre akeliekqfa irrtnsklde ikkqnredak qshvraaest
      481 dikikglavq vrehfyvqlr salidnyekm isdikgqsqe khlhsiaqdl eyrvlcntkv
      541 ankykfdmsk lissvrkcts tntlheclrd fnvssnnvtt ykskqevsnd slehdteses
      601 vinkfddkdi risfqessis kdipcstfkt alelrndrnl iagtsndsrn ylfdasilkd
      661 dskdvnaigr knkdrnspvk vdiknseedk tielqqlsgf tcarklheen nilsknyskk
      721 srkslaksvk ndsekkkasp tkksiyekil qaasanlkkt venttnssip easnnidsts
      781 lnkdeiklns qtrnkrknin sfkssidves nkklklnepq vqvlagdter isdhnngvna
      841 netstnetie iedrkddikd vqcqeelsmi ktveeeilre hknlqrkqdk thrskqqkge
      901 dkkvlvpadk atqfktaeil kahlmkyyps kripdrttfs ktcremhhkl lakkifdkeg
      961 irqfvikyma qn
//
LOCUS       XP_034182926             598 aa            linear   INV 03-SEP-2025
DEFINITION  scavenger receptor class B member 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182926
VERSION     XP_034182926.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327035.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..598
                     /product="scavenger receptor class B member 1 isoform X1"
                     /calculated_mol_wt=68837
     Region          78..527
                     /region_name="CD36"
                     /note="CD36 family; pfam01130"
                     /db_xref="CDD:460076"
     CDS             1..598
                     /gene="LOC117605562"
                     /coded_by="XM_034327035.2:582..2378"
                     /db_xref="GeneID:117605562"
ORIGIN      
        1 mkdfmmiwnh iagrryssvn vnqnqsdsse seeiainrtn nlrrkssivv nrffnatlsv
       61 htegkpkwpk sifmlmtlgs lglatgcilf vlqpydllfk lkvifsdgge ifelwrkpdv
      121 elyvkiylfn vtnrdeymsg kesklkfqei gpytyrevle hadvkfndng tvttfphhpl
      181 kyvpemsggr eedeailpni allsitnvmk dasyftrlgl nllimntnsq plvkmtakef
      241 mfgyestlvt lgnkmmpswi kfdklglidr mydfdgdyet vytgekdire tgliekyngd
      301 ynlpqwtgkc anvnhasdgv kfpsylepnd tvlffrkslc rsaymtqtee tyiqglhayk
      361 ykfvenvldn gaynpenkcf crkgyclkpg lidvtdcyyg fpialsyphf yksdpsllaa
      421 vkgltpradl hesfayvqpq sglplklafr fqinmalqni ghmarvekfe nfvlpllwfe
      481 igmyelptsm nirfwlylnf lpvlqciviy slfiigsili vlsihkilmy qlngstvsrq
      541 wldpeiqsnk lqcfnnnrrm sckvkemdly ystlleskda spiteespel sslkediv
//
LOCUS       XP_034182927             539 aa            linear   INV 03-SEP-2025
DEFINITION  scavenger receptor class B member 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182927
VERSION     XP_034182927.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327036.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..539
                     /product="scavenger receptor class B member 1 isoform X2"
                     /calculated_mol_wt=62093
     Region          19..468
                     /region_name="CD36"
                     /note="CD36 family; pfam01130"
                     /db_xref="CDD:460076"
     CDS             1..539
                     /gene="LOC117605562"
                     /coded_by="XM_034327036.2:161..1780"
                     /db_xref="GeneID:117605562"
ORIGIN      
        1 mfcyaripkl irifmlmtlg slglatgcil fvlqpydllf klkvifsdgg eifelwrkpd
       61 velyvkiylf nvtnrdeyms gkesklkfqe igpytyrevl ehadvkfndn gtvttfphhp
      121 lkyvpemsgg reedeailpn iallsitnvm kdasyftrlg lnllimntns qplvkmtake
      181 fmfgyestlv tlgnkmmpsw ikfdklglid rmydfdgdye tvytgekdir etgliekyng
      241 dynlpqwtgk canvnhasdg vkfpsylepn dtvlffrksl crsaymtqte etyiqglhay
      301 kykfvenvld ngaynpenkc fcrkgyclkp glidvtdcyy gfpialsyph fyksdpslla
      361 avkgltprad lhesfayvqp qsglplklaf rfqinmalqn ighmarvekf enfvlpllwf
      421 eigmyelpts mnirfwlyln flpvlqcivi yslfiigsil ivlsihkilm yqlngstvsr
      481 qwldpeiqsn klqcfnnnrr msckvkemdl yystlleskd aspiteespe lsslkediv
//
LOCUS       XP_076548162             525 aa            linear   INV 03-SEP-2025
DEFINITION  scavenger receptor class B member 1 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548162
VERSION     XP_076548162.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692047.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="scavenger receptor class B member 1 isoform X3"
                     /calculated_mol_wt=60340
     Region          5..454
                     /region_name="CD36"
                     /note="CD36 family; pfam01130"
                     /db_xref="CDD:460076"
     CDS             1..525
                     /gene="LOC117605562"
                     /coded_by="XM_076692047.1:200..1777"
                     /db_xref="GeneID:117605562"
ORIGIN      
        1 mlmtlgslgl atgcilfvlq pydllfklkv ifsdggeife lwrkpdvely vkiylfnvtn
       61 rdeymsgkes klkfqeigpy tyrevlehad vkfndngtvt tfphhplkyv pemsggreed
      121 eailpniall sitnvmkdas yftrlglnll imntnsqplv kmtakefmfg yestlvtlgn
      181 kmmpswikfd klglidrmyd fdgdyetvyt gekdiretgl iekyngdynl pqwtgkcanv
      241 nhasdgvkfp sylepndtvl ffrkslcrsa ymtqteetyi qglhaykykf venvldngay
      301 npenkcfcrk gyclkpglid vtdcyygfpi alsyphfyks dpsllaavkg ltpradlhes
      361 fayvqpqsgl plklafrfqi nmalqnighm arvekfenfv lpllwfeigm yelptsmnir
      421 fwlylnflpv lqciviyslf iigsilivls ihkilmyqln gstvsrqwld peiqsnklqc
      481 fnnnrrmsck vkemdlyyst lleskdaspi teespelssl kediv
//
LOCUS       XP_076548163             585 aa            linear   INV 03-SEP-2025
DEFINITION  fasciclin 3 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548163
VERSION     XP_076548163.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692048.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="fasciclin 3 isoform X1"
                     /calculated_mol_wt=64152
     Region          139..241
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          163..167
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          177..181
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          208..212
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          222..227
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          238..241
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..585
                     /gene="Fas3"
                     /coded_by="XM_076692048.1:239..1996"
                     /db_xref="GeneID:117605564"
ORIGIN      
        1 mcmestshfk mtitwvclia llcstavkgs ksnlvvdiep krqtavrlge slqilcrvgr
       61 plqvcrveip gedggmvlsk gqppedgiey ygegteagqc gvhiakikei hdgifkctlt
      121 ptdsraevqa svkiivakpp snpelhtspg sygrniyrkg eklevscsap sgrpaanvsl
      181 flddepigne erptiydsnv ddnslavqna srsldwtdng kilrcvashi aldkpkattm
      241 qlqvyyppqp qpqpierfgy vigrqgivnv tvyanprprf lwrvneekin egrpdesnrl
      301 etssavdlgr gawsailrid svqksdteka yilearndeg aneyrivlst stepagvdld
      361 agsiigivvg alvlilivfi lifaratgrw cfagtstsrn lgesksntir diadpaasvn
      421 llrfekskta ddedrqrqaf lfsfstlfle nrpnikltpm sisdtesagr ysrtevdgss
      481 kggrksrisl srlfkrnkdk vsgadtdtmr tvvtvddekl qtslatpqen ksnpstgegg
      541 ivyaeldltq qqqgvvtprv lnedkteyae ilytkpatee etpke
//
LOCUS       XP_034182930             560 aa            linear   INV 03-SEP-2025
DEFINITION  fasciclin 3 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034182930
VERSION     XP_034182930.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327039.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..560
                     /product="fasciclin 3 isoform X2"
                     /calculated_mol_wt=61254
     Region          139..241
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          163..167
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          177..181
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          208..212
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          222..227
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          238..241
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..560
                     /gene="Fas3"
                     /coded_by="XM_034327039.2:238..1920"
                     /db_xref="GeneID:117605564"
ORIGIN      
        1 mcmestshfk mtitwvclia llcstavkgs ksnlvvdiep krqtavrlge slqilcrvgr
       61 plqvcrveip gedggmvlsk gqppedgiey ygegteagqc gvhiakikei hdgifkctlt
      121 ptdsraevqa svkiivakpp snpelhtspg sygrniyrkg eklevscsap sgrpaanvsl
      181 flddepigne erptiydsnv ddnslavqna srsldwtdng kilrcvashi aldkpkattm
      241 qlqvyyppqp qpqpierfgy vigrqgivnv tvyanprprf lwrvneekin egrpdesnrl
      301 etssavdlgr gawsailrid svqksdteka yilearndeg aneyrivlst stepagvdld
      361 agsiigivvg alvlilivfi lifaratgrw cfagtstsrn lgesksntir diadpaasvn
      421 llrfekskta ddedrqrsdt esagrysrte vdgsskggrk srislsrlfk rnkdkvsgad
      481 tdtmrtvvtv ddeklqtsla tpqenksnps tgeggivyae ldltqqqqgv vtprvlnedk
      541 teyaeilytk pateeetpke
//
LOCUS       XP_034182931             527 aa            linear   INV 03-SEP-2025
DEFINITION  fasciclin 3 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034182931
VERSION     XP_034182931.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327040.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..527
                     /product="fasciclin 3 isoform X3"
                     /calculated_mol_wt=57511
     Region          139..241
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          163..167
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          177..181
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          208..212
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          222..227
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          238..241
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..527
                     /gene="Fas3"
                     /coded_by="XM_034327040.2:237..1820"
                     /db_xref="GeneID:117605564"
ORIGIN      
        1 mcmestshfk mtitwvclia llcstavkgs ksnlvvdiep krqtavrlge slqilcrvgr
       61 plqvcrveip gedggmvlsk gqppedgiey ygegteagqc gvhiakikei hdgifkctlt
      121 ptdsraevqa svkiivakpp snpelhtspg sygrniyrkg eklevscsap sgrpaanvsl
      181 flddepigne erptiydsnv ddnslavqna srsldwtdng kilrcvashi aldkpkattm
      241 qlqvyyppqp qpqpierfgy vigrqgivnv tvyanprprf lwrvneekin egrpdesnrl
      301 etssavdlgr gawsailrid svqksdteka yilearndeg aneyrivlst stepagvdld
      361 agsiigivvg alvlilivfi lifaratgrw cfagtstsrn lgessdtesa grysrtevdg
      421 sskggrksri slsrlfkrnk dkvsgadtdt mrtvvtvdde klqtslatpq enksnpstge
      481 ggivyaeldl tqqqqgvvtp rvlnedktey aeilytkpat eeetpke
//
LOCUS       XP_076548164             510 aa            linear   INV 03-SEP-2025
DEFINITION  fasciclin 3 isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076548164
VERSION     XP_076548164.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692049.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..510
                     /product="fasciclin 3 isoform X4"
                     /calculated_mol_wt=55965
     Region          64..166
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          88..92
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          102..106
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          133..137
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          147..152
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          163..166
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..510
                     /gene="Fas3"
                     /coded_by="XM_076692049.1:398..1930"
                     /db_xref="GeneID:117605564"
ORIGIN      
        1 mvlskgqppe dgieyygegt eagqcgvhia kikeihdgif kctltptdsr aevqasvkii
       61 vakppsnpel htspgsygrn iyrkgeklev scsapsgrpa anvslfldde pigneerpti
      121 ydsnvddnsl avqnasrsld wtdngkilrc vashialdkp kattmqlqvy yppqpqpqpi
      181 erfgyvigrq givnvtvyan prprflwrvn eekinegrpd esnrletssa vdlgrgawsa
      241 ilridsvqks dtekayilea rndeganeyr ivlststepa gvdldagsii givvgalvli
      301 livfilifar atgrwcfagt stsrnlgesk sntirdiadp aasvnllrfe ksktaddedr
      361 qrqaflfsfs tlflenrpni kltpmsisdt esagrysrte vdgsskggrk srislsrlfk
      421 rnkdkvsgad tdtmrtvvtv ddeklqtsla tpqenksnps tgeggivyae ldltqqqqgv
      481 vtprvlnedk teyaeilytk pateeetpke
//
LOCUS       XP_034182934             469 aa            linear   INV 03-SEP-2025
DEFINITION  fasciclin 3 isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034182934
VERSION     XP_034182934.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327043.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..469
                     /product="fasciclin 3 isoform X5"
                     /calculated_mol_wt=51160
     Region          139..241
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          163..167
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          177..181
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          208..212
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          222..227
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          238..241
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..469
                     /gene="Fas3"
                     /coded_by="XM_034327043.2:236..1645"
                     /db_xref="GeneID:117605564"
ORIGIN      
        1 mcmestshfk mtitwvclia llcstavkgs ksnlvvdiep krqtavrlge slqilcrvgr
       61 plqvcrveip gedggmvlsk gqppedgiey ygegteagqc gvhiakikei hdgifkctlt
      121 ptdsraevqa svkiivakpp snpelhtspg sygrniyrkg eklevscsap sgrpaanvsl
      181 flddepigne erptiydsnv ddnslavqna srsldwtdng kilrcvashi aldkpkattm
      241 qlqvyyppqp qpqpierfgy vigrqgivnv tvyanprprf lwrvneekin egrpdesnrl
      301 etssavdlgr gawsailrid svqksdteka yilearndeg aneyrivlst stepagvdld
      361 agsiigivvg alvlilivfi lifaratgrw cfaarrrgde eaasgvgags eahitpgded
      421 eedhedprii dekipqggqe npihasteyv ngrtdvketk kdektdtpv
//
LOCUS       XP_034182929             462 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605563 [Osmia lignaria lignaria].
ACCESSION   XP_034182929
VERSION     XP_034182929.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327038.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182929.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..462
                     /product="uncharacterized protein LOC117605563"
                     /calculated_mol_wt=53436
     CDS             1..462
                     /gene="LOC117605563"
                     /coded_by="XM_034327038.2:149..1537"
                     /db_xref="GeneID:117605563"
ORIGIN      
        1 mnskilqldr kklqdisski sevrarrdly kmkhdqtatq lhdcndmklv wqhlmpstck
       61 dldpkvliev ldtvtslwtg tskrevwnti snnldrmevp tlwhhlyqnl tkdvdsliks
      121 asmksmdtdk ksinigiari ygqhicmisk qllyntrakk hqqnvlelie kievasnnsa
      181 disewlalel evckleseqi nlqeevdkir kslyesnift idlsqitsei qninlekmdc
      241 iqeiqqslsl lkaaptflmk vkqkinlele kittmringy dllelnndlt teldmfheal
      301 dlnalrkiil kgdigvyrht ksciseasis vtnsqtsnir syfptvqipl yslidcykni
      361 ilmltckrie cleieenpsl lpipilahke nnyntvelfk lsesfntktk aeiaefneil
      421 nawihqtvqk vmeiidktvd datfsewier ydlllymlqn st
//
LOCUS       XP_034182936             116 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605566 [Osmia lignaria lignaria].
ACCESSION   XP_034182936
VERSION     XP_034182936.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327045.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182936.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..116
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..116
                     /product="uncharacterized protein LOC117605566"
                     /calculated_mol_wt=13299
     CDS             1..116
                     /gene="LOC117605566"
                     /coded_by="XM_034327045.2:483..833"
                     /db_xref="GeneID:117605566"
ORIGIN      
        1 mayvtdmskv yhhlgpreaa tvyntkgdvl rttvsefikf vlnedetpfy nwhllepgky
       61 fqsernlgin qeeklqrqvd gsdmppysip prssgiinrl pkfrcrllra siqavi
//
LOCUS       XP_034182935             224 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605565 [Osmia lignaria lignaria].
ACCESSION   XP_034182935
VERSION     XP_034182935.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327044.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..224
                     /product="uncharacterized protein LOC117605565"
                     /calculated_mol_wt=26157
     CDS             1..224
                     /gene="LOC117605565"
                     /coded_by="XM_034327044.2:1..675"
                     /db_xref="GeneID:117605565"
ORIGIN      
        1 msrridcrry whwkrqqesk sekekrkakk rkrresitdc iddveqseaa evvfkvghpk
       61 hkkkkktpwi pavqlikrvp ladlvlksik psmeirdyfh nprhtaclql tsldidiapk
      121 iietttslys qtqmvgqikd rkkcgssvrr ktvddenenr rqlrkissrk dieewesnde
      181 sdcisicsld sviclqgtkl tskdkeeikk yqsqaqansd keee
//
LOCUS       XP_034190749             483 aa            linear   INV 03-SEP-2025
DEFINITION  lim3 homeobox protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190749
VERSION     XP_034190749.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334858.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190749.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="lim3 homeobox protein isoform X1"
                     /calculated_mol_wt=51447
     Region          111..162
                     /region_name="LIM1_Lhx3_Lhx4"
                     /note="The first LIM domain of Lhx3 and Lhx4 family;
                     cd09368"
                     /db_xref="CDD:188754"
     Site            order(111,114,132,135,138,141,158,161)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188754"
     Region          170..225
                     /region_name="LIM2_Lhx3_Lhx4"
                     /note="The second LIM domain of Lhx3-Lhx4 family; cd09376"
                     /db_xref="CDD:188762"
     Site            order(170,173,192,195,198,201,221,224)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188762"
     Site            order(182,207)
                     /site_type="other"
                     /note="Isl binding site"
                     /db_xref="CDD:188762"
     Region          246..302
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..483
                     /gene="Lim3"
                     /coded_by="XM_034334858.2:3251..4702"
                     /db_xref="GeneID:117609024"
ORIGIN      
        1 msaavqpvat lptlpssgpp phnplhhtpg glplpplgsa pvmmprppat tmpslgpslp
       61 pshqdtgdla eaapnqdvll allarnknle erkiktpscf pikeepsipk cggcqeaild
      121 kyvlrvlerc wharcltcrd cgarltdkcf arnghvfckd dffkrfgtkc agcgqglaps
      181 qvvrraqelv yhltcfscal csrqldtgde fylmedrklv ckpdyeqaka keladggsid
      241 gdqpnkrprt titakqletl klayntspkp arhvreqlsq dtgldmrvvq vwfqnrrake
      301 krlkkdagrt rwsqyfrsmk gtgagghspr hdklldkdel kvdldstfgh hdlsndsygt
      361 vvnmgldgeg aspggggnga gggpprgylg attppylsts rspslppqfp yppdaglsvy
      421 ttlggaggmv pgpasdlsne ssgggggggg gggggypdfp pspdswlgep ppphahhhsh
      481 yat
//
LOCUS       XP_034190753             463 aa            linear   INV 03-SEP-2025
DEFINITION  lim3 homeobox protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190753
VERSION     XP_034190753.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334862.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190753.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..463
                     /product="lim3 homeobox protein isoform X2"
                     /calculated_mol_wt=50062
     Region          91..142
                     /region_name="LIM1_Lhx3_Lhx4"
                     /note="The first LIM domain of Lhx3 and Lhx4 family;
                     cd09368"
                     /db_xref="CDD:188754"
     Site            order(91,94,112,115,118,121,138,141)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188754"
     Region          150..205
                     /region_name="LIM2_Lhx3_Lhx4"
                     /note="The second LIM domain of Lhx3-Lhx4 family; cd09376"
                     /db_xref="CDD:188762"
     Site            order(150,153,172,175,178,181,201,204)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188762"
     Site            order(162,187)
                     /site_type="other"
                     /note="Isl binding site"
                     /db_xref="CDD:188762"
     Region          226..282
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..463
                     /gene="Lim3"
                     /coded_by="XM_034334862.2:1111..2502"
                     /db_xref="GeneID:117609024"
ORIGIN      
        1 mtmislemlg realslegmy rrarqdiltp phdspesqnv rrnssnagnt vnvtnfekad
       61 hnemhpldqp alelglpahi peilyssipk cggcqeaild kyvlrvlerc wharcltcrd
      121 cgarltdkcf arnghvfckd dffkrfgtkc agcgqglaps qvvrraqelv yhltcfscal
      181 csrqldtgde fylmedrklv ckpdyeqaka keladggsid gdqpnkrprt titakqletl
      241 klayntspkp arhvreqlsq dtgldmrvvq vwfqnrrake krlkkdagrt rwsqyfrsmk
      301 gtgagghspr hdklldkdel kvdldstfgh hdlsndsygt vvnmgldgeg aspggggnga
      361 gggpprgylg attppylsts rspslppqfp yppdaglsvy ttlggaggmv pgpasdlsne
      421 ssgggggggg gggggypdfp pspdswlgep ppphahhhsh yat
//
LOCUS       XP_034183042             577 aa            linear   INV 03-SEP-2025
DEFINITION  neurotrophin 1 [Osmia lignaria lignaria].
ACCESSION   XP_034183042
VERSION     XP_034183042.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327151.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183042.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="neurotrophin 1"
                     /calculated_mol_wt=65497
     Region          264..352
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..577
                     /gene="NT1"
                     /coded_by="XM_034327151.2:370..2103"
                     /db_xref="GeneID:117605621"
ORIGIN      
        1 marglllitv avvllssinv caeedlkddg dlndrkenng ttvtsikitg egsknrtdkt
       61 retqlerlrs lfasrqnssr dltlpkssdi akvisddvtg deetpeelpe pqelkiirpn
      121 lrkrpvfrps psrrplppnr rhvygdlsrt pltrpprrpa evkrdpteke ctfftktvcl
      181 eaadypheai srslrsnkem vaslltdyks qdfdesgenf pialplenkf enkydgryen
      241 ryesneirrr sdmpspfdni eegftcpsvv kyarpqlara asgiwkyiin tgehtqtlrl
      301 ekcsnpqssc sfisenyrss cvqvynyhrl ltwdsklglh mdifkvptcc schvhgyadi
      361 fpphqsdppp rikesfpgsd fattndqkdd fedvsklnyl nkyvagfdsa lgssnkkpls
      421 easpsrptft lpvrtrpkkq psssnsrpfd klpqqhapnt rapgytgpll kgsrpsrpsr
      481 ppfrrestsh ieenteslns talnrysqpy dlemdassrl qnggfddeyh epqrrinyny
      541 hpiidffrpe asmlqtaetq sasdqndsns wkpmiss
//
LOCUS       XP_034190754             430 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine aminotransferase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190754
VERSION     XP_034190754.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334863.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..430
                     /product="tyrosine aminotransferase isoform X1"
                     /calculated_mol_wt=48274
     Region          10..410
                     /region_name="tyr_amTase_E"
                     /note="tyrosine aminotransferase, eukaryotic; TIGR01264"
                     /db_xref="CDD:273529"
     CDS             1..430
                     /gene="Tat"
                     /coded_by="XM_034334863.2:240..1532"
                     /db_xref="GeneID:117609026"
ORIGIN      
        1 mstlisrerw nveaseiarn thnpirsive slvvepnpak svialsigdp ttfgnlkppk
       61 eaidavqesl lsqsyngyap stgyqmakea vaeysssefv dvdpkdvilc sgcscaldlc
      121 italarrdqn iliprpgfsi yrtlaeglgi tvksyelrpe fgweidlndl esqidestaa
      181 iiinnpsnpc gsvfsrnhtl dildvaaryy vpiiadeiye hmvfpgrtfh slaslsnevp
      241 ilscsgltkr flvpgwrmgw iiihdrqnvl nkeirkalhc lsqriigsnt liqgalpail
      301 kntpqnfydn imhtlynhsk vaynciikvp glkpimpdga mymmvhidls cfpefnsdle
      361 fvqrllmees vfclpgqcfd ypsymrlvit vpmdlleeac qriqefcerh hyktaehtrr
      421 nsfldgeisc
//
LOCUS       XP_034190755             387 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine aminotransferase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190755
VERSION     XP_034190755.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334864.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..387
                     /product="tyrosine aminotransferase isoform X2"
                     /calculated_mol_wt=43410
     Region          10..367
                     /region_name="tyr_amTase_E"
                     /note="tyrosine aminotransferase, eukaryotic; TIGR01264"
                     /db_xref="CDD:273529"
     CDS             1..387
                     /gene="Tat"
                     /coded_by="XM_034334864.2:242..1405"
                     /db_xref="GeneID:117609026"
ORIGIN      
        1 mstlisrerw nveaseiarn thnpirsive slvvepnpak svialsigdp ttfgnlkppk
       61 eaidavqesl lsqsyngyap stgyqmakea vaeysssefv dvdpkdvilc sgcscaldlc
      121 italarrdqn iliprpgfsi yrtlaeglgi tvksyelrpe fgweidlndl esqidestaa
      181 iiinnpsnpc gsvfsrnhtl dildvaaryy vpiiadeiye hmvfpgrtfh slaslsnevp
      241 ilscsgltkr flvpgwrmgw iiihdrqnvl nkenhskvay nciikvpglk pimpdgamym
      301 mvhidlscfp efnsdlefvq rllmeesvfc lpgqcfdyps ymrlvitvpm dlleeacqri
      361 qefcerhhyk taehtrrnsf ldgeisc
//
LOCUS       XP_034190760             479 aa            linear   INV 03-SEP-2025
DEFINITION  popeye domain-containing protein 1-like [Osmia lignaria lignaria].
ACCESSION   XP_034190760
VERSION     XP_034190760.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334869.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="popeye domain-containing protein 1-like"
                     /calculated_mol_wt=52907
     Region          122..330
                     /region_name="Popeye"
                     /note="Popeye protein conserved region; pfam04831"
                     /db_xref="CDD:461448"
     CDS             1..479
                     /gene="LOC117609033"
                     /coded_by="XM_034334869.2:1200..2639"
                     /db_xref="GeneID:117609033"
ORIGIN      
        1 marttrsrrk manttdapsp assllssttt tpftsptstt ystegytfny tglsslggss
       61 fedlnytelw vdvwnsteas nvterlnati lssgggycde weaaqhklfq aanlffaaaf
      121 lvprsfkasv lalrtfltag fmlaalwagi ticaldamlw clalgllngi hslilacrfl
      181 ppalspelae lylklfkpyk vskkhfqela kearilklds gqtyategvt paderlsill
      241 rgklkvtcdg thlhhikayq fvdspeweat heniddvfqv tiraeessty icwtrlkllr
      301 vlrhrpllkv vlntligkdi tsklyalneq lagvaaaset ttsnpyrgva rslsvdavnt
      361 etagrvrstt wkaqrrhsnp rsdrsspsry shqywapvva nhfpptspft pgqnlgysll
      421 pqgvqfsppp rapllshqpl trqrsggtsg dytllpqtpk lerkrskkgt revtfetpv
//
LOCUS       XP_034183044             110 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605622 [Osmia lignaria lignaria].
ACCESSION   XP_034183044
VERSION     XP_034183044.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327153.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..110
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..110
                     /product="uncharacterized protein LOC117605622"
                     /calculated_mol_wt=11559
     CDS             1..110
                     /gene="LOC117605622"
                     /coded_by="XM_034327153.2:114..446"
                     /db_xref="GeneID:117605622"
ORIGIN      
        1 mlplkpvllf tvlgllklst aapdsagive mvnglayggi pygsvtgmva kypgfmrqqi
       61 qarqlnafra vqplavntnf gasrlvgvqp qtvqiynvpg vvgtirqlgy
//
LOCUS       XP_034183022             750 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605607 [Osmia lignaria lignaria].
ACCESSION   XP_034183022
VERSION     XP_034183022.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327131.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183022.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..750
                     /product="uncharacterized protein LOC117605607"
                     /calculated_mol_wt=85235
     Region          14..89
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); pfam07776"
                     /db_xref="CDD:462262"
     Region          <221..>401
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     Region          293..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(298,300,302,304..305,308..309,312,324,326,330..331,
                     334..335,338,351,353,355,357..358,361..362,365)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          317..696
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          319..339
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          346..366
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          420..440
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          447..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          518..538
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          547..567
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          579..599
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(584,586,588,590..591,594..595,598,612,614,618..619,
                     622..623,626,640,642,644,646..647,650..651,654)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          607..627
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          635..655
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          663..683
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          691..709
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..750
                     /gene="LOC117605607"
                     /coded_by="XM_034327131.2:269..2521"
                     /db_xref="GeneID:117605607"
ORIGIN      
        1 mteyfdlpln mdricriclt egtnlspifs tdqevndfsd lpqkiqvcgs ieiheqdglp
       61 slicdiciyk asvahefrqq cqhsdaklrm yynkptkvti vdtctqtese ittalskeqv
      121 seedyfvkee mqgasnsqef iqtndaycrn estlntgqie mqgeklfics igfegtkete
      181 keagtsendi hqtcvtqest ddvsetidme nksediqdts qimeednkke edkfldesis
      241 nddvkeeevl qkrtssrkvk svpnktysed eiddsyydls ndgqdsgdsk fkckvcskcf
      301 stqrglkkhs lvhekkhkcn icskmfykqe nmenhkkiha skplacqlch acfskpqslv
      361 rhlkshtekv ndmikqintd eqkdskepkk emkledntdd esgtvnevyn fenapelykc
      421 eicsqycssl knlkrhtlvh gdkkysctvc kkwffrpdtl kkhaekhghg lldnlvdnnk
      481 ffdsdddscp ntttnnaqes esvkkeesde dgsgeykcqh cdkimatkkg lrrhvsthkp
      541 kaepvtceic gkicasqarl vlhqrthvpk ekvpreylch icskvypsns sltyhmrtht
      601 gikphvcktc nsgfttttsl anhirihtgd kpfvchvcsa afavssafrr hltrhtgean
      661 ylcktcgkaf krlstlkeht ythsgekpyv cktcgaaysh sgslfahqkr craqygeivv
      721 dehhhsvahh ihvnnvqsav rslavigqmf
//
LOCUS       XP_034183023             750 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605607 [Osmia lignaria lignaria].
ACCESSION   XP_034183023
VERSION     XP_034183023.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327132.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183023.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..750
                     /product="uncharacterized protein LOC117605607"
                     /calculated_mol_wt=85235
     Region          14..89
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); pfam07776"
                     /db_xref="CDD:462262"
     Region          <221..>401
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     Region          293..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(298,300,302,304..305,308..309,312,324,326,330..331,
                     334..335,338,351,353,355,357..358,361..362,365)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          317..696
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          319..339
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          346..366
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          420..440
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          447..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          518..538
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          547..567
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          579..599
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(584,586,588,590..591,594..595,598,612,614,618..619,
                     622..623,626,640,642,644,646..647,650..651,654)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          607..627
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          635..655
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          663..683
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          691..709
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..750
                     /gene="LOC117605607"
                     /coded_by="XM_034327132.2:209..2461"
                     /db_xref="GeneID:117605607"
ORIGIN      
        1 mteyfdlpln mdricriclt egtnlspifs tdqevndfsd lpqkiqvcgs ieiheqdglp
       61 slicdiciyk asvahefrqq cqhsdaklrm yynkptkvti vdtctqtese ittalskeqv
      121 seedyfvkee mqgasnsqef iqtndaycrn estlntgqie mqgeklfics igfegtkete
      181 keagtsendi hqtcvtqest ddvsetidme nksediqdts qimeednkke edkfldesis
      241 nddvkeeevl qkrtssrkvk svpnktysed eiddsyydls ndgqdsgdsk fkckvcskcf
      301 stqrglkkhs lvhekkhkcn icskmfykqe nmenhkkiha skplacqlch acfskpqslv
      361 rhlkshtekv ndmikqintd eqkdskepkk emkledntdd esgtvnevyn fenapelykc
      421 eicsqycssl knlkrhtlvh gdkkysctvc kkwffrpdtl kkhaekhghg lldnlvdnnk
      481 ffdsdddscp ntttnnaqes esvkkeesde dgsgeykcqh cdkimatkkg lrrhvsthkp
      541 kaepvtceic gkicasqarl vlhqrthvpk ekvpreylch icskvypsns sltyhmrtht
      601 gikphvcktc nsgfttttsl anhirihtgd kpfvchvcsa afavssafrr hltrhtgean
      661 ylcktcgkaf krlstlkeht ythsgekpyv cktcgaaysh sgslfahqkr craqygeivv
      721 dehhhsvahh ihvnnvqsav rslavigqmf
//
LOCUS       XP_034183024             750 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605607 [Osmia lignaria lignaria].
ACCESSION   XP_034183024
VERSION     XP_034183024.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327133.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183024.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..750
                     /product="uncharacterized protein LOC117605607"
                     /calculated_mol_wt=85235
     Region          14..89
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); pfam07776"
                     /db_xref="CDD:462262"
     Region          <221..>401
                     /region_name="SCP-1"
                     /note="Synaptonemal complex protein 1 (SCP-1); pfam05483"
                     /db_xref="CDD:114219"
     Region          293..313
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(298,300,302,304..305,308..309,312,324,326,330..331,
                     334..335,338,351,353,355,357..358,361..362,365)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          317..696
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          319..339
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          346..366
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          420..440
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          447..467
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          518..538
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          547..567
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          579..599
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(584,586,588,590..591,594..595,598,612,614,618..619,
                     622..623,626,640,642,644,646..647,650..651,654)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          607..627
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          635..655
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          663..683
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          691..709
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..750
                     /gene="LOC117605607"
                     /coded_by="XM_034327133.2:97..2349"
                     /db_xref="GeneID:117605607"
ORIGIN      
        1 mteyfdlpln mdricriclt egtnlspifs tdqevndfsd lpqkiqvcgs ieiheqdglp
       61 slicdiciyk asvahefrqq cqhsdaklrm yynkptkvti vdtctqtese ittalskeqv
      121 seedyfvkee mqgasnsqef iqtndaycrn estlntgqie mqgeklfics igfegtkete
      181 keagtsendi hqtcvtqest ddvsetidme nksediqdts qimeednkke edkfldesis
      241 nddvkeeevl qkrtssrkvk svpnktysed eiddsyydls ndgqdsgdsk fkckvcskcf
      301 stqrglkkhs lvhekkhkcn icskmfykqe nmenhkkiha skplacqlch acfskpqslv
      361 rhlkshtekv ndmikqintd eqkdskepkk emkledntdd esgtvnevyn fenapelykc
      421 eicsqycssl knlkrhtlvh gdkkysctvc kkwffrpdtl kkhaekhghg lldnlvdnnk
      481 ffdsdddscp ntttnnaqes esvkkeesde dgsgeykcqh cdkimatkkg lrrhvsthkp
      541 kaepvtceic gkicasqarl vlhqrthvpk ekvpreylch icskvypsns sltyhmrtht
      601 gikphvcktc nsgfttttsl anhirihtgd kpfvchvcsa afavssafrr hltrhtgean
      661 ylcktcgkaf krlstlkeht ythsgekpyv cktcgaaysh sgslfahqkr craqygeivv
      721 dehhhsvahh ihvnnvqsav rslavigqmf
//
LOCUS       XP_034183315             300 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605749 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183315
VERSION     XP_034183315.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327424.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183315.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..300
                     /product="uncharacterized protein LOC117605749 isoform X1"
                     /calculated_mol_wt=34090
     CDS             1..300
                     /gene="LOC117605749"
                     /coded_by="XM_034327424.2:126..1028"
                     /db_xref="GeneID:117605749"
ORIGIN      
        1 mpklnkllvt siiadirecv cvaneiyswv ggiehlswkf ptkiardina meivkeyegt
       61 agefyillle laydriqfll rlivefldky pfiddffkev tcvnrpnqis lanllrlvwd
      121 rlktipesis rqmvknsqke aeecftpkvs rscqaggsmn ycescriage tvlqaintie
      181 eifvenklis vaaaqrneck snliertqws vmskltkaig ediemalkkm rnsnlkieav
      241 kreheksire lqsnlttqkk rvdtlqvknl elenalengr fpasgtgtga detnrcnvet
//
LOCUS       XP_034183316             205 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605749 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183316
VERSION     XP_034183316.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327425.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183316.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..205
                     /product="uncharacterized protein LOC117605749 isoform X2"
                     /calculated_mol_wt=23759
     Region          <62..>121
                     /region_name="COG2433"
                     /note="Possible nuclease of RNase H fold, RuvC/YqgF family
                     [General function prediction only]"
                     /db_xref="CDD:441980"
     CDS             1..205
                     /gene="LOC117605749"
                     /coded_by="XM_034327425.2:117..734"
                     /db_xref="GeneID:117605749"
ORIGIN      
        1 magfrlvere leltkrtdvt lkrenetlke tmedsrrqis nltggnsvlq sqvatlvesk
       61 lelektvirl rkevidtrda cleterrtem llnesrkend smrdehrkei erlqqeiasl
      121 rtttaiktkn ekmknnscsn nseglradti lmpednpvad mtrqlisnre kievlsrqne
      181 rlsktlhrlr eyrlmgqitt ngsnk
//
LOCUS       XP_034187073             488 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607463 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187073
VERSION     XP_034187073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..488
                     /product="uncharacterized protein LOC117607463 isoform X1"
                     /calculated_mol_wt=58054
     Region          <212..410
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..488
                     /gene="LOC117607463"
                     /coded_by="XM_034331182.2:330..1796"
                     /db_xref="GeneID:117607463"
ORIGIN      
        1 meltetnvqi rdrsinlipv yikqendehe tdfndveiin aaqdfcaiei keskectain
       61 snkesnvnnk ncrqhtnsln stlnvvhqne ncningilng svylqnldye ninieiqkpt
      121 dqiaevfidq neqpqttnys vstvtipsel lglnlnikke neldqetvya tewlcsvsae
      181 sdlrlkvskn tkekanistd idatitlltk qkdripennn nlktkestpe rsrkqlklrs
      241 nslqdykekl rrkaecmrek rkklyeqese eqrqqrlake aakrremrmy yetpeqrrkr
      301 ldveaarkre yrmynetped rrkrldreae rrrlkrlsly asetpeqrre rlnresakrr
      361 earhnqyake teeerkerlk rdalrvremr ftrsaietee erkqrlvkda lrkrevrmhg
      421 rhsvnnnfte lqtvrnfeel nnvqikenpd nqlgghylnn wmmwfqnsiv qpvhvgeqii
      481 ksqlenne
//
LOCUS       XP_034187074             405 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607463 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187074
VERSION     XP_034187074.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331183.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..405
                     /product="uncharacterized protein LOC117607463 isoform X2"
                     /calculated_mol_wt=48203
     CDS             1..405
                     /gene="LOC117607463"
                     /coded_by="XM_034331183.2:330..1547"
                     /db_xref="GeneID:117607463"
ORIGIN      
        1 meltetnvqi rdrsinlipv yikqendehe tdfndveiin aaqdfcaiei keskectain
       61 snkesnvnnk ncrqhtnsln stlnvvhqne ncningilng svylqnldye ninieiqkpt
      121 dqiaevfidq neqpqttnys vstvtipsel lglnlnikke neldqetvya tewlcsvsae
      181 sdlrlkvskn tkekanistd idatitlltk qkdripennn nlktkestpe rsrkqlklrs
      241 nslqdykekl rrkaecmrek rkklyeqese eqrqqrlake aakrremrmy yetpeqrrkr
      301 ldveaarkre yrmynetped rrkrldreae rrrlkrlsly asetpeqrre rlnresakrr
      361 earhnqyake teeerkerlk rdalrvremr ftrsaietee erkqr
//
LOCUS       XP_034190729             483 aa            linear   INV 03-SEP-2025
DEFINITION  RRM1_TatSF1_like and RRM2_TatSF1_like domain-containing protein
            barc isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190729
VERSION     XP_034190729.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334838.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="RRM1_TatSF1_like and RRM2_TatSF1_like
                     domain-containing protein barc isoform X1"
                     /calculated_mol_wt=55590
     Region          160..249
                     /region_name="RRM1_TatSF1_like"
                     /note="RNA recognition motif 1 (RRM1) found in HIV
                     Tat-specific factor 1 (Tat-SF1) and similar proteins;
                     cd12281"
                     /db_xref="CDD:409723"
     Region          291..381
                     /region_name="RRM2_TatSF1_like"
                     /note="RNA recognition motif 2 (RRM2) found in HIV
                     Tat-specific factor 1 (Tat-SF1) and similar proteins;
                     cd12282"
                     /db_xref="CDD:409724"
     CDS             1..483
                     /gene="barc"
                     /coded_by="XM_034334838.2:177..1628"
                     /db_xref="GeneID:117609012"
ORIGIN      
        1 madcqeqekp ddpmklyefd gesyiykdpt tnvtyrfdqe knewvekkte etkgndsstn
       61 eakpqegvfg fendthtytd pndgsvyfwd keknawfpkv dddfmaryqm sygftdsstt
      121 etkpvqqpet kkaekekrkv eakrkaqepp twfevdeahn taiyvsglpl ditlpeltel
      181 fskcgliard ekgkdkikly rdsngqpkgd alciyikves vdlalkildk sqlrgktlsv
      241 qrakfqmkge aydpalkpkr kkkdkerqkk iheklfdwrp erplgepgkh ekvviiknlf
      301 spedfdkeva llleyqqdir seclkcgdvr kvviydrhpe gvaqvtfrep aeaqacvqll
      361 ngrwfsqrki saeiwdgktk ykvtetdaei eariakwdkf legeeekkea aarneqrsne
      421 ksmaekseaf qgktelpett lkasaektea snveeprkkt ktadetetss nsddsdegdr
      481 nnq
//
LOCUS       XP_034190730             483 aa            linear   INV 03-SEP-2025
DEFINITION  RRM1_TatSF1_like and RRM2_TatSF1_like domain-containing protein
            barc isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190730
VERSION     XP_034190730.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334839.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..483
                     /product="RRM1_TatSF1_like and RRM2_TatSF1_like
                     domain-containing protein barc isoform X1"
                     /calculated_mol_wt=55590
     Region          160..249
                     /region_name="RRM1_TatSF1_like"
                     /note="RNA recognition motif 1 (RRM1) found in HIV
                     Tat-specific factor 1 (Tat-SF1) and similar proteins;
                     cd12281"
                     /db_xref="CDD:409723"
     Region          291..381
                     /region_name="RRM2_TatSF1_like"
                     /note="RNA recognition motif 2 (RRM2) found in HIV
                     Tat-specific factor 1 (Tat-SF1) and similar proteins;
                     cd12282"
                     /db_xref="CDD:409724"
     CDS             1..483
                     /gene="barc"
                     /coded_by="XM_034334839.2:199..1650"
                     /db_xref="GeneID:117609012"
ORIGIN      
        1 madcqeqekp ddpmklyefd gesyiykdpt tnvtyrfdqe knewvekkte etkgndsstn
       61 eakpqegvfg fendthtytd pndgsvyfwd keknawfpkv dddfmaryqm sygftdsstt
      121 etkpvqqpet kkaekekrkv eakrkaqepp twfevdeahn taiyvsglpl ditlpeltel
      181 fskcgliard ekgkdkikly rdsngqpkgd alciyikves vdlalkildk sqlrgktlsv
      241 qrakfqmkge aydpalkpkr kkkdkerqkk iheklfdwrp erplgepgkh ekvviiknlf
      301 spedfdkeva llleyqqdir seclkcgdvr kvviydrhpe gvaqvtfrep aeaqacvqll
      361 ngrwfsqrki saeiwdgktk ykvtetdaei eariakwdkf legeeekkea aarneqrsne
      421 ksmaekseaf qgktelpett lkasaektea snveeprkkt ktadetetss nsddsdegdr
      481 nnq
//
LOCUS       XP_076548167             476 aa            linear   INV 03-SEP-2025
DEFINITION  RRM1_TatSF1_like and RRM2_TatSF1_like domain-containing protein
            barc isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548167
VERSION     XP_076548167.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692052.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..476
                     /product="RRM1_TatSF1_like and RRM2_TatSF1_like
                     domain-containing protein barc isoform X2"
                     /calculated_mol_wt=55549
     Region          160..249
                     /region_name="RRM1_TatSF1_like"
                     /note="RNA recognition motif 1 (RRM1) found in HIV
                     Tat-specific factor 1 (Tat-SF1) and similar proteins;
                     cd12281"
                     /db_xref="CDD:409723"
     Region          291..381
                     /region_name="RRM2_TatSF1_like"
                     /note="RNA recognition motif 2 (RRM2) found in HIV
                     Tat-specific factor 1 (Tat-SF1) and similar proteins;
                     cd12282"
                     /db_xref="CDD:409724"
     CDS             1..476
                     /gene="barc"
                     /coded_by="XM_076692052.1:177..1607"
                     /db_xref="GeneID:117609012"
ORIGIN      
        1 madcqeqekp ddpmklyefd gesyiykdpt tnvtyrfdqe knewvekkte etkgndsstn
       61 eakpqegvfg fendthtytd pndgsvyfwd keknawfpkv dddfmaryqm sygftdsstt
      121 etkpvqqpet kkaekekrkv eakrkaqepp twfevdeahn taiyvsglpl ditlpeltel
      181 fskcgliard ekgkdkikly rdsngqpkgd alciyikves vdlalkildk sqlrgktlsv
      241 qrakfqmkge aydpalkpkr kkkdkerqkk iheklfdwrp erplgepgkh ekvviiknlf
      301 spedfdkeva llleyqqdir seclkcgdvr kvviydrhpe gvaqvtfrep aeaqacvqll
      361 ngrwfsqrki saeiwdgktk yklfktiksv seskiqmiar eeakkmlavl vksicflglq
      421 rltrrskpel qngtsfwrar rrrkrlprgt nsvamknqwr rnlrlskekr sfpkrr
//
LOCUS       XP_034190761             780 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609034 [Osmia lignaria lignaria].
ACCESSION   XP_034190761
VERSION     XP_034190761.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334870.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190761.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..780
                     /product="uncharacterized protein LOC117609034"
                     /calculated_mol_wt=84864
     Region          39..367
                     /region_name="ATS1"
                     /note="Alpha-tubulin suppressor ATS1 and related RCC1
                     domain-containing proteins [Cell cycle control, cell
                     division, chromosome partitioning, Cytoskeleton]; COG5184"
                     /db_xref="CDD:444065"
     Region          440..>670
                     /region_name="PRK08581"
                     /note="amidase domain-containing protein"
                     /db_xref="CDD:236304"
     CDS             1..780
                     /gene="LOC117609034"
                     /coded_by="XM_034334870.2:150..2492"
                     /db_xref="GeneID:117609034"
ORIGIN      
        1 mglsdletip esgavftvgr srfadniash ffirkdpvis itcgdehsav icqsgrlfvf
       61 gsndwgqlgl ghrnhiskps cvkvlkpekv thvacgraht lictgeqkif acgtdqegql
      121 grgtsaigdf ssspvlvyds glagskilql aagshhslal isdggvfawg snlegqlglp
      181 dvsglvnkpt kvqipepvkq isagyyhsaf ltesgsvyvc gesesgklgi nvnfstqvtp
      241 kqmqlpspav hvacgghhtv ilvengnvyc tgsnsngqlg lgtnvtelqt pkllsrgmlq
      301 ndtisriscg eshtaivtgs gklftcgdgr hgklgleene nnvheltfvq rykelfvskv
      361 acggchtilv gqrretdnya erdketsqte hvqkrnslpp lklpvnilqt ephedtikaq
      421 inpentdlps tikteldnvt nneenmnsvt dsmnniekve spkessngtk qktsestgne
      481 meperaskem pevaeesees kvqnenhesi nenkkvepkv deennikeet tpeetnteqn
      541 nkennnemse ndaadhkeis ekdtaidnks ntekndipes mnekieskdt nddkektnte
      601 kqmspssstp ppkpprlkpg sgnstsgert ssmekeeend kvedderked ttekeaetke
      661 dnevkkqets smksstkkqe sssmkssaks ksskstrsiv dtedaieidk qddkektvea
      721 ermnndiaqd dadvvqspap rtggrmskif kakkpqgven gtkstgavnh kpksktcsil
//
LOCUS       XP_034190763             404 aa            linear   INV 03-SEP-2025
DEFINITION  yellow-f [Osmia lignaria lignaria].
ACCESSION   XP_034190763
VERSION     XP_034190763.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334872.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190763.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..404
                     /product="yellow-f"
                     /calculated_mol_wt=45700
     Region          110..392
                     /region_name="MRJP"
                     /note="Major royal jelly protein; pfam03022"
                     /db_xref="CDD:308585"
     CDS             1..404
                     /gene="Y-f"
                     /coded_by="XM_034334872.2:194..1408"
                     /db_xref="GeneID:117609035"
ORIGIN      
        1 mwclllvafi vvanghdfkt iyswnyvdfd fpnesirdsl issghyipen nmplglqswn
       61 dkffitiprw kngvvsnlny isknaqsqsp klipypnwem ncidfpgsiv sifrtkvdac
      121 drlwgvdtgi tdilgnatvv qpvrifvfdl ktdkvlrvyt lkdsdqtsns ffadavvdvd
      181 pnncdnahly isdlsgygiv vyswakndsw rithnyfhfd plhgdynisg ynfqwtdgvf
      241 glslstprnd gyktlyfham sgitefsvst evlqddtlkr ssdyhtfhve gkkgarsqgp
      301 tslidtktgi dyftqvskng vacwdtsvpl dedsfvlvaq dnttlvfpqd isidplfnml
      361 yvlsdnlpql mfsnydpktr nffltaadld sltpackkqn sksr
//
LOCUS       XP_034191069            1089 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191069
VERSION     XP_034191069.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335178.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191069.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1089
                     /product="serine/threonine kinase isoform X1"
                     /calculated_mol_wt=118894
     Region          508..764
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(508..511,514,516,529,531,558,574..577,621,625..626,
                     628,643..644)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1089
                     /gene="cdi"
                     /coded_by="XM_034335178.2:1242..4511"
                     /db_xref="GeneID:117609181"
ORIGIN      
        1 mfgkvppsnt pgppcscdss sksgpttclp cfsvdetssm lteekskawr skdscahvvs
       61 eppstgmeek qklwrskenv vfeapciled kskskntlve dksrsswrlk ensstglpev
      121 sspveekska rkcketcsat esvddkkcwk skesisplet ptddkskprr askdssnies
      181 ccgsqtvqpn lsqsstafve ansrccstkn ssskyskets hgrnhtglvt ksnsvvhkcs
      241 snpncpnftk spgcsggsks qrvlcstsls slnvdpgsre acklskkhsg pcrelaagke
      301 qhltggkcvs regqrnssqa gclasksssr ssdaaysasn gknravsess detadssmdf
      361 svisviqstp sescsrssak gygdkdtvds saaggncdrs crgdfttstp skdwmkngda
      421 lqqncvsclp qdlptelqfs prsyrerekc rdpwrpteme snicnlnvcp sdtyqdtgtk
      481 rrtgascqal rhavaslnrl ddfymekiga gffsevfkvt hkvtsqvmvl kmnqlpanrp
      541 nmlkevqlmn klshpnilrf mgvcvhegql halteyingg sleqlimarh tplphlirmk
      601 lardvsrgma ylhsrglfhr dltsknvlik kdentsemta vvgdfglaak ipdpssgyrl
      661 stvgspywms peclkgqwyd hrsdvfsfgi vvceligrvp adpdvlprsd nfgldylava
      721 eicaaadppp aflqlafncc tyepksrptf seittsldnm isnyedeskn nickrqsvdp
      781 tlmssmdeit regrsmkrkt arlrsqsada rgcdnatpsd karchsarrv aelasrrdph
      841 yrpmtanpfh alggvkkilg dlfssclelp sledvrpsvt daiakfkpaq ndsiakildr
      901 kkpssepssp tarkkwerkv atkvgaaslf thplfrdgwe prrrgscesg fwscvgedls
      961 peppnrrhts tlsssaassl fllddhrtss iytdssedia slgggdscwe drlggigsss
     1021 ktiskiveyf erkqagslrf adhdvgsarl tllkaslegs vplcsisaaq rlvvcegavr
     1081 sklplfdkk
//
LOCUS       XP_034191070            1089 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine kinase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191070
VERSION     XP_034191070.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335179.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191070.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1089
                     /product="serine/threonine kinase isoform X1"
                     /calculated_mol_wt=118894
     Region          508..764
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(508..511,514,516,529,531,558,574..577,621,625..626,
                     628,643..644)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..1089
                     /gene="cdi"
                     /coded_by="XM_034335179.2:633..3902"
                     /db_xref="GeneID:117609181"
ORIGIN      
        1 mfgkvppsnt pgppcscdss sksgpttclp cfsvdetssm lteekskawr skdscahvvs
       61 eppstgmeek qklwrskenv vfeapciled kskskntlve dksrsswrlk ensstglpev
      121 sspveekska rkcketcsat esvddkkcwk skesisplet ptddkskprr askdssnies
      181 ccgsqtvqpn lsqsstafve ansrccstkn ssskyskets hgrnhtglvt ksnsvvhkcs
      241 snpncpnftk spgcsggsks qrvlcstsls slnvdpgsre acklskkhsg pcrelaagke
      301 qhltggkcvs regqrnssqa gclasksssr ssdaaysasn gknravsess detadssmdf
      361 svisviqstp sescsrssak gygdkdtvds saaggncdrs crgdfttstp skdwmkngda
      421 lqqncvsclp qdlptelqfs prsyrerekc rdpwrpteme snicnlnvcp sdtyqdtgtk
      481 rrtgascqal rhavaslnrl ddfymekiga gffsevfkvt hkvtsqvmvl kmnqlpanrp
      541 nmlkevqlmn klshpnilrf mgvcvhegql halteyingg sleqlimarh tplphlirmk
      601 lardvsrgma ylhsrglfhr dltsknvlik kdentsemta vvgdfglaak ipdpssgyrl
      661 stvgspywms peclkgqwyd hrsdvfsfgi vvceligrvp adpdvlprsd nfgldylava
      721 eicaaadppp aflqlafncc tyepksrptf seittsldnm isnyedeskn nickrqsvdp
      781 tlmssmdeit regrsmkrkt arlrsqsada rgcdnatpsd karchsarrv aelasrrdph
      841 yrpmtanpfh alggvkkilg dlfssclelp sledvrpsvt daiakfkpaq ndsiakildr
      901 kkpssepssp tarkkwerkv atkvgaaslf thplfrdgwe prrrgscesg fwscvgedls
      961 peppnrrhts tlsssaassl fllddhrtss iytdssedia slgggdscwe drlggigsss
     1021 ktiskiveyf erkqagslrf adhdvgsarl tllkaslegs vplcsisaaq rlvvcegavr
     1081 sklplfdkk
//
LOCUS       XP_034191071             953 aa            linear   INV 03-SEP-2025
DEFINITION  serine/threonine kinase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191071
VERSION     XP_034191071.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335180.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191071.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..953
                     /product="serine/threonine kinase isoform X2"
                     /calculated_mol_wt=104721
     Region          508..764
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     Site            order(508..511,514,516,529,531,558,574..577,621,625..626,
                     628,643..644)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     CDS             1..953
                     /gene="cdi"
                     /coded_by="XM_034335180.2:1242..4103"
                     /db_xref="GeneID:117609181"
ORIGIN      
        1 mfgkvppsnt pgppcscdss sksgpttclp cfsvdetssm lteekskawr skdscahvvs
       61 eppstgmeek qklwrskenv vfeapciled kskskntlve dksrsswrlk ensstglpev
      121 sspveekska rkcketcsat esvddkkcwk skesisplet ptddkskprr askdssnies
      181 ccgsqtvqpn lsqsstafve ansrccstkn ssskyskets hgrnhtglvt ksnsvvhkcs
      241 snpncpnftk spgcsggsks qrvlcstsls slnvdpgsre acklskkhsg pcrelaagke
      301 qhltggkcvs regqrnssqa gclasksssr ssdaaysasn gknravsess detadssmdf
      361 svisviqstp sescsrssak gygdkdtvds saaggncdrs crgdfttstp skdwmkngda
      421 lqqncvsclp qdlptelqfs prsyrerekc rdpwrpteme snicnlnvcp sdtyqdtgtk
      481 rrtgascqal rhavaslnrl ddfymekiga gffsevfkvt hkvtsqvmvl kmnqlpanrp
      541 nmlkevqlmn klshpnilrf mgvcvhegql halteyingg sleqlimarh tplphlirmk
      601 lardvsrgma ylhsrglfhr dltsknvlik kdentsemta vvgdfglaak ipdpssgyrl
      661 stvgspywms peclkgqwyd hrsdvfsfgi vvceligrvp adpdvlprsd nfgldylava
      721 eicaaadppp aflqlafncc tyepksrptf seittsldnm isnyedeskn nickrqsvdp
      781 tlmssmdeit regrsmkrkt arlrsqsada rgcdnatpsd karchsarrv aelasrrdph
      841 yrpmtanpfh alggvkkilg dlfssclelp sledvrpsvt daiakfkpaq ndsiakildr
      901 kkpssepssp tarkkwerkv reksflcnfc grkraidier rfrvvslned dlh
//
LOCUS       XP_034191072            1030 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609182 [Osmia lignaria lignaria].
ACCESSION   XP_034191072
VERSION     XP_034191072.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335181.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191072.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1030
                     /product="uncharacterized protein LOC117609182"
                     /calculated_mol_wt=115001
     Region          98..391
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            213..217
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(215,238,323)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(298..299,320..321)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..1030
                     /gene="LOC117609182"
                     /coded_by="XM_034335181.2:831..3923"
                     /db_xref="GeneID:117609182"
ORIGIN      
        1 migklvlslw ilgcisldgd paeaqewptp ldesadeknd aeifravves mrewslhkkl
       61 hqrikrevsk vcyedvgcfe dtgpfsylem lpsppkevgt rflvygsrka rsipmevpad
      121 dindnahrai dpdlptkviv hgfgsscdhv wvyemrsalm tvhecnivcv dwgpgsavpn
      181 yvraaantrl vgrqlaklir smnvplekvh ligfslgahv agfagaelgn vsritgldpa
      241 gplfesqdpr vrldktdanf vdvihsngeq lllgglgswq pmgdvdfypn ggrmqtgcsn
      301 lflgavsdii wssavegrsl cnhrrayklf tdsvspkcrf pafpcehgyd gllrgdcfpc
      361 ggngmgkpcg dmgyysdess argqlylvtr deepfcahqy qvkvynsrre rptrsygklq
      421 vtlvgdgtfn dsfamtrkde ellvgsilqk ivvphpaiss meaieikyta ysgwissglv
      481 swsidkvaii dsfgktlsic rkgltlesgk pvylplfpge cniplesdns tspvlgrllq
      541 ekqggigpft keqnydpkds fsvesfskdr tssvssskgl gpftkeqnlr lierkesfed
      601 igsrrsstnp wnivdisadg dsnslensda ekgrgfsssn fftkpsssdr siestteflp
      661 eirepvlqvn kketgrslkf seitepilrp rsarqnsrne vhvrdkqdei qetssttrsf
      721 tvqflperla gilaqaerya rqtllplisq ytpsfmtgtr yeepkyfppl geitsedyqd
      781 katvnedede adesnetaks ehssenvsee vsaiveevyr dnggsnssrg epnnewvpie
      841 psvtvssrkd ynvsrslnwe herynwstqs epesndfdtr iddwvpitik nevrtsqssn
      901 nasndvptgd kdmeinvqge svttedskne sndsgkderk fiplvdpeee esttttsndv
      961 setrsstysh iqkiprptgt rnnqssgnlt rrsmvfpyay eknkdprtry iplipeedlg
     1021 rsrwsfdrer
//
LOCUS       XP_034191074             828 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609183 [Osmia lignaria lignaria].
ACCESSION   XP_034191074
VERSION     XP_034191074.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335183.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="uncharacterized protein LOC117609183"
                     /calculated_mol_wt=93534
     CDS             1..828
                     /gene="LOC117609183"
                     /coded_by="XM_034335183.2:398..2884"
                     /db_xref="GeneID:117609183"
ORIGIN      
        1 mlpssralls slllliiags tgafnsrqge ctfplkwegt wfqsgvrqpi visrnelssk
       61 grclhnegdk fllvdqkscy rcvvihekhs nvlqyketyc hgksslsslc sfitgdally
      121 smfreeatpv pcpfrgpmtf synrghgtcs vpvsnvdtct ddsrllfryq acpditases
      181 aveeleclat wkegssrylv grlhhghass nedryrcfvy ekagqtvqgn lhraamgmga
      241 mdhdvslqsg pvpegaaeiy rvaqsgdatc nglfspmegs rtmtlmkvsp pgqcrfpnwl
      301 tghssggltw htldltrsyt fhprnaslha trsnsstsrf esgsldgqyv pgqedqdvki
      361 lcnsikqsss aqavtmtmlv ahftvgcrsg fmcmtfyrrd ghvielqtgs avsrpeeacs
      421 pphfqqhnip fltlvtsspe prtcpylgkf tvtgvnrnqr niresrrnqh qeavrrdkek
      481 vrhaqersqe drrgrkldfd aekrranney lvhermgrra rsnrnnrshr dhsieeessk
      541 rssdiedlll erswskvrrs dvqrtselat rskvrrtrdl etrtrekskr npkiedfrld
      601 addfartgqf wtsdfddsdd ssiinvqidy fgdypdtgdl pkkrsdrnpe eityellsrt
      661 rrdaeeldav dgsfekrekr eedisrcsse vttitigcst adrmefqtdc vednaiaysc
      721 hgrwfdaegt qfviatqlap gttwtnennr pqpyrnsrrl cfmykesggv vsltasevac
      781 qrgipppppm lafnatstgq cmeenrgfvv rstylmivil tavvaiyr
//
LOCUS       XP_034191073             828 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609183 [Osmia lignaria lignaria].
ACCESSION   XP_034191073
VERSION     XP_034191073.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="uncharacterized protein LOC117609183"
                     /calculated_mol_wt=93534
     CDS             1..828
                     /gene="LOC117609183"
                     /coded_by="XM_034335182.2:393..2879"
                     /db_xref="GeneID:117609183"
ORIGIN      
        1 mlpssralls slllliiags tgafnsrqge ctfplkwegt wfqsgvrqpi visrnelssk
       61 grclhnegdk fllvdqkscy rcvvihekhs nvlqyketyc hgksslsslc sfitgdally
      121 smfreeatpv pcpfrgpmtf synrghgtcs vpvsnvdtct ddsrllfryq acpditases
      181 aveeleclat wkegssrylv grlhhghass nedryrcfvy ekagqtvqgn lhraamgmga
      241 mdhdvslqsg pvpegaaeiy rvaqsgdatc nglfspmegs rtmtlmkvsp pgqcrfpnwl
      301 tghssggltw htldltrsyt fhprnaslha trsnsstsrf esgsldgqyv pgqedqdvki
      361 lcnsikqsss aqavtmtmlv ahftvgcrsg fmcmtfyrrd ghvielqtgs avsrpeeacs
      421 pphfqqhnip fltlvtsspe prtcpylgkf tvtgvnrnqr niresrrnqh qeavrrdkek
      481 vrhaqersqe drrgrkldfd aekrranney lvhermgrra rsnrnnrshr dhsieeessk
      541 rssdiedlll erswskvrrs dvqrtselat rskvrrtrdl etrtrekskr npkiedfrld
      601 addfartgqf wtsdfddsdd ssiinvqidy fgdypdtgdl pkkrsdrnpe eityellsrt
      661 rrdaeeldav dgsfekrekr eedisrcsse vttitigcst adrmefqtdc vednaiaysc
      721 hgrwfdaegt qfviatqlap gttwtnennr pqpyrnsrrl cfmykesggv vsltasevac
      781 qrgipppppm lafnatstgq cmeenrgfvv rstylmivil tavvaiyr
//
LOCUS       XP_034191076             828 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609183 [Osmia lignaria lignaria].
ACCESSION   XP_034191076
VERSION     XP_034191076.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335185.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="uncharacterized protein LOC117609183"
                     /calculated_mol_wt=93534
     CDS             1..828
                     /gene="LOC117609183"
                     /coded_by="XM_034335185.2:119..2605"
                     /db_xref="GeneID:117609183"
ORIGIN      
        1 mlpssralls slllliiags tgafnsrqge ctfplkwegt wfqsgvrqpi visrnelssk
       61 grclhnegdk fllvdqkscy rcvvihekhs nvlqyketyc hgksslsslc sfitgdally
      121 smfreeatpv pcpfrgpmtf synrghgtcs vpvsnvdtct ddsrllfryq acpditases
      181 aveeleclat wkegssrylv grlhhghass nedryrcfvy ekagqtvqgn lhraamgmga
      241 mdhdvslqsg pvpegaaeiy rvaqsgdatc nglfspmegs rtmtlmkvsp pgqcrfpnwl
      301 tghssggltw htldltrsyt fhprnaslha trsnsstsrf esgsldgqyv pgqedqdvki
      361 lcnsikqsss aqavtmtmlv ahftvgcrsg fmcmtfyrrd ghvielqtgs avsrpeeacs
      421 pphfqqhnip fltlvtsspe prtcpylgkf tvtgvnrnqr niresrrnqh qeavrrdkek
      481 vrhaqersqe drrgrkldfd aekrranney lvhermgrra rsnrnnrshr dhsieeessk
      541 rssdiedlll erswskvrrs dvqrtselat rskvrrtrdl etrtrekskr npkiedfrld
      601 addfartgqf wtsdfddsdd ssiinvqidy fgdypdtgdl pkkrsdrnpe eityellsrt
      661 rrdaeeldav dgsfekrekr eedisrcsse vttitigcst adrmefqtdc vednaiaysc
      721 hgrwfdaegt qfviatqlap gttwtnennr pqpyrnsrrl cfmykesggv vsltasevac
      781 qrgipppppm lafnatstgq cmeenrgfvv rstylmivil tavvaiyr
//
LOCUS       XP_034191077             570 aa            linear   INV 03-SEP-2025
DEFINITION  nucleostemin 1 [Osmia lignaria lignaria].
ACCESSION   XP_034191077
VERSION     XP_034191077.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335186.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191077.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="nucleostemin 1"
                     /calculated_mol_wt=64261
     Region          15..88
                     /region_name="GN3L_Grn1"
                     /note="GNL3L/Grn1 putative GTPase; pfam08701"
                     /db_xref="CDD:462569"
     Region          146..316
                     /region_name="Nucleostemin_like"
                     /note="A circularly permuted subfamily of the Ras GTPases;
                     cd04178"
                     /db_xref="CDD:206753"
     Site            order(183..184,186..187,212..214,270..276,315)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206753"
     Site            183..186
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206753"
     Site            212..214
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206753"
     Site            268..275
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206753"
     Site            293..300
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206753"
     Site            296
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206753"
     Site            312..315
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206753"
     Site            314..316
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206753"
     CDS             1..570
                     /gene="Ns1"
                     /coded_by="XM_034335186.2:144..1856"
                     /db_xref="GeneID:117609184"
ORIGIN      
        1 makfclkkts krmparkryk iekkvrehnr klkkeakkhp ktkrkvievp nqcpfkenil
       61 keveamkkqh deerqkqrea arekkkqela kggleglvsa aqhkqaehke metdtvheni
      121 knvlakeens lkayykefkk vldaadvile vvdardplgt rckeveeavr sakgnkrlvi
      181 vlnkadlvpr enlnqwlkyl rstlpavafk sstqdqskrl grrklgkkte dmiqsgtcfg
      241 aelllsllan ycrnvggvkt sirvgvvglp nvgkssvins lkrsracnvg ntpgvtkamq
      301 avqldskikl ldspgivfan nsgengdegs ialknavkiq slkdpftpai tilkriskpq
      361 ilelynvqdf ttpdeffamk atrmgrfkkg gipdtvaaar gilddwnsgk iryytvppeq
      421 pdcqvsaeiv sqiskefdie svvtqekmml dtfevesknk paiepllies sgpvtaamev
      481 emqketqvki kkkvekakqn knetrkkkvd plfeiegnqk lnklnklqfk kekkdrarre
      541 kvavkladql egfnitgpdd ydfstdfvek
//
LOCUS       XP_034191078             413 aa            linear   INV 03-SEP-2025
DEFINITION  DNA fragmentation factor-related protein 4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191078
VERSION     XP_034191078.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335187.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..413
                     /product="DNA fragmentation factor-related protein 4"
                     /calculated_mol_wt=47625
     Region          20..93
                     /region_name="CIDE_N"
                     /note="CIDE_N domain, found at the N-terminus of the CIDE
                     (cell death-inducing DFF45-like effector) proteins, as
                     well as CAD nuclease (caspase-activated DNase/DNA
                     fragmentation factor, DFF40) and its inhibitor,
                     ICAD(DFF45). These proteins are associated with...;
                     cl02541"
                     /db_xref="CDD:470607"
     Site            order(24,33..34,36..37,47,51,67,69..70,74..75)
                     /site_type="other"
                     /note="putative heterodimer interaction sites [polypeptide
                     binding]"
                     /db_xref="CDD:119367"
     Region          116..413
                     /region_name="DFF40"
                     /note="DNA fragmentation factor 40 kDa; pfam09230"
                     /db_xref="CDD:462717"
     CDS             1..413
                     /gene="Drep4"
                     /coded_by="XM_034335187.2:425..1666"
                     /db_xref="GeneID:117609185"
ORIGIN      
        1 mslitgcfrr lteaptrtel kgykvtdvnr trkfgvacrs lqelkrkaca klnvtndlae
       61 vnvflldgsl ideeyfstle pqttiiiqkp gekvltdadl lydtlrrvni dfltagikas
      121 efltehlktk vailnnalnk ddskttfstr dehpewfrgl etncttkeay mhrkcqdrvr
      181 gylyktidqi kssetfakdp rarkqllyai affkmqlked hyfghyfdrt rakmeslnsv
      241 desdssvcyd hcpcrlscld gstyfrlfgt mnqscvdgar kkgtqtdsis deeqeaenny
      301 pytvkrndrv scvamcdhkg efrccgiwna ekcsygdrhk inpyrsreel ilfstwnfdh
      361 kiersrtlip mllklssqgi anetdildiy dnlftlknlr lvhivchdkg shk
//
LOCUS       XP_034191079             403 aa            linear   INV 03-SEP-2025
DEFINITION  crustacean cardioactive peptide receptor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191079
VERSION     XP_034191079.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335188.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="crustacean cardioactive peptide receptor isoform
                     X1"
                     /calculated_mol_wt=45411
     Region          51..337
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          53..77
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          86..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          124..146
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          167..183
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          209..232
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          270..292
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          306..331
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..403
                     /gene="CCAP-R"
                     /coded_by="XM_034335188.2:1740..2951"
                     /db_xref="GeneID:117609186"
ORIGIN      
        1 msvernveei drnengnrte nrssylpeff nitlpdvnat deidsfyfye teqftvlwll
       61 famivvgnia vligllwgkr rktrmdffik qlafadllvg lisvltdiiw rttvtwhagn
      121 vacklirfmq avvtysstyv lvalsidryd aitrpmnftg rwwraralvi gawslsalfs
      181 vpiiflyeek riqgktqcwi dlgsplqwri ymslvsftlf iapaliiggc yavivatiws
      241 qggalrqgpt rdtrrassrg liprakvktv kmtlvivfvf ilcwspyivf dllqvygyvp
      301 etqtniavat fiqsltplns aanpiiyclf stsfcktvrn mqaitwvsgw catnphhcfg
      361 tggpnsssrt tvttsltahs srrsghiaml hsttrkrvmv slv
//
LOCUS       XP_034191080             403 aa            linear   INV 03-SEP-2025
DEFINITION  crustacean cardioactive peptide receptor isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191080
VERSION     XP_034191080.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335189.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..403
                     /product="crustacean cardioactive peptide receptor isoform
                     X1"
                     /calculated_mol_wt=45411
     Region          51..337
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          53..77
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          86..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          124..146
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          167..183
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          209..232
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          270..292
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          306..331
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..403
                     /gene="CCAP-R"
                     /coded_by="XM_034335189.2:1651..2862"
                     /db_xref="GeneID:117609186"
ORIGIN      
        1 msvernveei drnengnrte nrssylpeff nitlpdvnat deidsfyfye teqftvlwll
       61 famivvgnia vligllwgkr rktrmdffik qlafadllvg lisvltdiiw rttvtwhagn
      121 vacklirfmq avvtysstyv lvalsidryd aitrpmnftg rwwraralvi gawslsalfs
      181 vpiiflyeek riqgktqcwi dlgsplqwri ymslvsftlf iapaliiggc yavivatiws
      241 qggalrqgpt rdtrrassrg liprakvktv kmtlvivfvf ilcwspyivf dllqvygyvp
      301 etqtniavat fiqsltplns aanpiiyclf stsfcktvrn mqaitwvsgw catnphhcfg
      361 tggpnsssrt tvttsltahs srrsghiaml hsttrkrvmv slv
//
LOCUS       XP_034191081             389 aa            linear   INV 03-SEP-2025
DEFINITION  crustacean cardioactive peptide receptor isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191081
VERSION     XP_034191081.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335190.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="crustacean cardioactive peptide receptor isoform
                     X2"
                     /calculated_mol_wt=43883
     Region          51..323
                     /region_name="7tm_GPCRs"
                     /note="seven-transmembrane G protein-coupled receptor
                     superfamily; cl28897"
                     /db_xref="CDD:475119"
     Region          53..77
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:410628"
     Region          86..109
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:410628"
     Region          124..146
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:410628"
     Region          167..183
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:410628"
     Region          195..218
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:410628"
     Region          256..278
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:410628"
     Region          292..317
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:410628"
     CDS             1..389
                     /gene="CCAP-R"
                     /coded_by="XM_034335190.2:1740..2909"
                     /db_xref="GeneID:117609186"
ORIGIN      
        1 msvernveei drnengnrte nrssylpeff nitlpdvnat deidsfyfye teqftvlwll
       61 famivvgnia vligllwgkr rktrmdffik qlafadllvg lisvltdiiw rttvtwhagn
      121 vacklirfmq avvtysstyv lvalsidryd aitrpmnftg rwwraralvi gawslsalfs
      181 vpiiflyeek riqwriymsl vsftlfiapa liiggcyavi vatiwsqgga lrqgptrdtr
      241 rassrglipr akvktvkmtl vivfvfilcw spyivfdllq vygyvpetqt niavatfiqs
      301 ltplnsaanp iiyclfstsf cktvrnmqai twvsgwcatn phhcfgtggp nsssrttvtt
      361 sltahssrrs ghiamlhstt rkrvmvslv
//
LOCUS       XP_034191082             390 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal cystine transporter cystinosin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191082
VERSION     XP_034191082.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191082.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="lysosomal cystine transporter cystinosin isoform
                     X1"
                     /calculated_mol_wt=44885
     Region          128..359
                     /region_name="2A43"
                     /note="Lysosomal Cystine Transporter; TIGR00951"
                     /db_xref="CDD:130026"
     CDS             1..390
                     /gene="Ctns"
                     /coded_by="XM_034335191.2:557..1729"
                     /db_xref="GeneID:117609187"
ORIGIN      
        1 maflqyfgif vllivyqvha kvpelkvstq dikvhlhske sfelyltqnl tesvlvkiev
       61 qhpdlvatep anfsvnasds nykwniiikg lgaghsvvav nvtpsnvtdf sdafvrvtve
      121 rsdiiyhvst vvgwvyflaw svsfypqiys nykrksvvgl nfdylslnli gfimyslfnc
      181 glywipevel eyfrrypkgl npvqvndiff slhavfatvi tiiqcfiyei gnqkvsitar
      241 iihvifavfi listilakvq titwldflyy csyvklcitl ikyvpqafyn ykrkstvgws
      301 ignifldftg gilsmlqmil naynyddwes ifgdptkfgl gffsvafdif filqhyvfyr
      361 dsqnarsnei lhrqensdde etrtccgfik
//
LOCUS       XP_034191083             390 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal cystine transporter cystinosin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191083
VERSION     XP_034191083.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335192.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191083.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="lysosomal cystine transporter cystinosin isoform
                     X1"
                     /calculated_mol_wt=44885
     Region          128..359
                     /region_name="2A43"
                     /note="Lysosomal Cystine Transporter; TIGR00951"
                     /db_xref="CDD:130026"
     CDS             1..390
                     /gene="Ctns"
                     /coded_by="XM_034335192.2:430..1602"
                     /db_xref="GeneID:117609187"
ORIGIN      
        1 maflqyfgif vllivyqvha kvpelkvstq dikvhlhske sfelyltqnl tesvlvkiev
       61 qhpdlvatep anfsvnasds nykwniiikg lgaghsvvav nvtpsnvtdf sdafvrvtve
      121 rsdiiyhvst vvgwvyflaw svsfypqiys nykrksvvgl nfdylslnli gfimyslfnc
      181 glywipevel eyfrrypkgl npvqvndiff slhavfatvi tiiqcfiyei gnqkvsitar
      241 iihvifavfi listilakvq titwldflyy csyvklcitl ikyvpqafyn ykrkstvgws
      301 ignifldftg gilsmlqmil naynyddwes ifgdptkfgl gffsvafdif filqhyvfyr
      361 dsqnarsnei lhrqensdde etrtccgfik
//
LOCUS       XP_034191085             390 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal cystine transporter cystinosin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191085
VERSION     XP_034191085.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335194.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191085.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="lysosomal cystine transporter cystinosin isoform
                     X1"
                     /calculated_mol_wt=44885
     Region          128..359
                     /region_name="2A43"
                     /note="Lysosomal Cystine Transporter; TIGR00951"
                     /db_xref="CDD:130026"
     CDS             1..390
                     /gene="Ctns"
                     /coded_by="XM_034335194.2:315..1487"
                     /db_xref="GeneID:117609187"
ORIGIN      
        1 maflqyfgif vllivyqvha kvpelkvstq dikvhlhske sfelyltqnl tesvlvkiev
       61 qhpdlvatep anfsvnasds nykwniiikg lgaghsvvav nvtpsnvtdf sdafvrvtve
      121 rsdiiyhvst vvgwvyflaw svsfypqiys nykrksvvgl nfdylslnli gfimyslfnc
      181 glywipevel eyfrrypkgl npvqvndiff slhavfatvi tiiqcfiyei gnqkvsitar
      241 iihvifavfi listilakvq titwldflyy csyvklcitl ikyvpqafyn ykrkstvgws
      301 ignifldftg gilsmlqmil naynyddwes ifgdptkfgl gffsvafdif filqhyvfyr
      361 dsqnarsnei lhrqensdde etrtccgfik
//
LOCUS       XP_034191086             390 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal cystine transporter cystinosin isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191086
VERSION     XP_034191086.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335195.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191086.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="lysosomal cystine transporter cystinosin isoform
                     X1"
                     /calculated_mol_wt=44885
     Region          128..359
                     /region_name="2A43"
                     /note="Lysosomal Cystine Transporter; TIGR00951"
                     /db_xref="CDD:130026"
     CDS             1..390
                     /gene="Ctns"
                     /coded_by="XM_034335195.2:500..1672"
                     /db_xref="GeneID:117609187"
ORIGIN      
        1 maflqyfgif vllivyqvha kvpelkvstq dikvhlhske sfelyltqnl tesvlvkiev
       61 qhpdlvatep anfsvnasds nykwniiikg lgaghsvvav nvtpsnvtdf sdafvrvtve
      121 rsdiiyhvst vvgwvyflaw svsfypqiys nykrksvvgl nfdylslnli gfimyslfnc
      181 glywipevel eyfrrypkgl npvqvndiff slhavfatvi tiiqcfiyei gnqkvsitar
      241 iihvifavfi listilakvq titwldflyy csyvklcitl ikyvpqafyn ykrkstvgws
      301 ignifldftg gilsmlqmil naynyddwes ifgdptkfgl gffsvafdif filqhyvfyr
      361 dsqnarsnei lhrqensdde etrtccgfik
//
LOCUS       XP_034191087             386 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal cystine transporter cystinosin isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191087
VERSION     XP_034191087.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335196.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191087.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="lysosomal cystine transporter cystinosin isoform
                     X2"
                     /calculated_mol_wt=44317
     Region          128..359
                     /region_name="2A43"
                     /note="Lysosomal Cystine Transporter; TIGR00951"
                     /db_xref="CDD:130026"
     CDS             1..386
                     /gene="Ctns"
                     /coded_by="XM_034335196.2:531..1691"
                     /db_xref="GeneID:117609187"
ORIGIN      
        1 maflqyfgif vllivyqvha kvpelkvstq dikvhlhske sfelyltqnl tesvlvkiev
       61 qhpdlvatep anfsvnasds nykwniiikg lgaghsvvav nvtpsnvtdf sdafvrvtve
      121 rsdiiyhvst vvgwvyflaw svsfypqiys nykrksvvgl nfdylslnli gfimyslfnc
      181 glywipevel eyfrrypkgl npvqvndiff slhavfatvi tiiqcfiyei gnqkvsitar
      241 iihvifavfi listilakvq titwldflyy csyvklcitl ikyvpqafyn ykrkstvgws
      301 ignifldftg gilsmlqmil naynyddwes ifgdptkfgl gffsvafdif filqhyvfyr
      361 drkeyveipg nnyegsqent ststlp
//
LOCUS       XP_034191088             373 aa            linear   INV 03-SEP-2025
DEFINITION  lysosomal cystine transporter cystinosin isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191088
VERSION     XP_034191088.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335197.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191088.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..373
                     /product="lysosomal cystine transporter cystinosin isoform
                     X3"
                     /calculated_mol_wt=43008
     Region          128..359
                     /region_name="2A43"
                     /note="Lysosomal Cystine Transporter; TIGR00951"
                     /db_xref="CDD:130026"
     CDS             1..373
                     /gene="Ctns"
                     /coded_by="XM_034335197.2:557..1678"
                     /db_xref="GeneID:117609187"
ORIGIN      
        1 maflqyfgif vllivyqvha kvpelkvstq dikvhlhske sfelyltqnl tesvlvkiev
       61 qhpdlvatep anfsvnasds nykwniiikg lgaghsvvav nvtpsnvtdf sdafvrvtve
      121 rsdiiyhvst vvgwvyflaw svsfypqiys nykrksvvgl nfdylslnli gfimyslfnc
      181 glywipevel eyfrrypkgl npvqvndiff slhavfatvi tiiqcfiyei gnqkvsitar
      241 iihvifavfi listilakvq titwldflyy csyvklcitl ikyvpqafyn ykrkstvgws
      301 ignifldftg gilsmlqmil naynyddwes ifgdptkfgl gffsvafdif filqhyvfyr
      361 finekmvdlk nri
//
LOCUS       XP_034191089             319 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191089
VERSION     XP_034191089.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..319
                     /product="very long chain fatty acid elongase 7 isoform
                     X1"
                     /calculated_mol_wt=37259
     Region          68..306
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..319
                     /gene="LOC117609188"
                     /coded_by="XM_034335198.2:22..981"
                     /db_xref="GeneID:117609188"
ORIGIN      
        1 mesfevlttp trrshasafp tlgarlpply rvycntfirk trrtmsgivd wykdivynkp
       61 dprtsdwflv tgpgplfmii vtyvyfsvsa gprymkdkkp yelrnvmily nfiqvllsiy
      121 lvyeglmagw ggqysyrcqp vdysnnpmal riargvhlyy lcklfelldt vffvlrkkqr
      181 qisflhvyhh slmpfcawig vrylpgghgt llgvinafih vimyayymls sigphmnkyl
      241 wwkkylttlq liqfsiiflh nlqvffngcn fpkpisfllc lnalifiymf ssfyvetytk
      301 krvsatkect ngtvknkpe
//
LOCUS       XP_034191090             290 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191090
VERSION     XP_034191090.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335199.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..290
                     /product="very long chain fatty acid elongase 7 isoform
                     X2"
                     /calculated_mol_wt=33992
     Region          39..277
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..290
                     /gene="LOC117609188"
                     /coded_by="XM_034335199.2:5431..6303"
                     /db_xref="GeneID:117609188"
ORIGIN      
        1 mnaikklfir ktrrtmsgiv dwykdivynk pdprtsdwfl vtgpgplfmi ivtyvyfsvs
       61 agprymkdkk pyelrnvmil ynfiqvllsi ylvyeglmag wggqysyrcq pvdysnnpma
      121 lriargvhly ylcklfelld tvffvlrkkq rqisflhvyh hslmpfcawi gvrylpgghg
      181 tllgvinafi hvimyayyml ssigphmnky lwwkkylttl qliqfsiifl hnlqvffngc
      241 nfpkpisfll clnalifiym fssfyvetyt kkrvsatkec tngtvknkpe
//
LOCUS       XP_034191091             275 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191091
VERSION     XP_034191091.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335200.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="very long chain fatty acid elongase 7 isoform
                     X3"
                     /calculated_mol_wt=32134
     Region          24..262
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..275
                     /gene="LOC117609188"
                     /coded_by="XM_034335200.2:901..1728"
                     /db_xref="GeneID:117609188"
ORIGIN      
        1 msgivdwykd ivynkpdprt sdwflvtgpg plfmiivtyv yfsvsagpry mkdkkpyelr
       61 nvmilynfiq vllsiylvye glmagwggqy syrcqpvdys nnpmalriar gvhlyylckl
      121 felldtvffv lrkkqrqisf lhvyhhslmp fcawigvryl pgghgtllgv inafihvimy
      181 ayymlssigp hmnkylwwkk ylttlqliqf siiflhnlqv ffngcnfpkp isfllclnal
      241 ifiymfssfy vetytkkrvs atkectngtv knkpe
//
LOCUS       XP_076548168             275 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548168
VERSION     XP_076548168.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692053.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="very long chain fatty acid elongase 7 isoform
                     X3"
                     /calculated_mol_wt=32134
     Region          24..262
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..275
                     /gene="LOC117609188"
                     /coded_by="XM_076692053.1:1082..1909"
                     /db_xref="GeneID:117609188"
ORIGIN      
        1 msgivdwykd ivynkpdprt sdwflvtgpg plfmiivtyv yfsvsagpry mkdkkpyelr
       61 nvmilynfiq vllsiylvye glmagwggqy syrcqpvdys nnpmalriar gvhlyylckl
      121 felldtvffv lrkkqrqisf lhvyhhslmp fcawigvryl pgghgtllgv inafihvimy
      181 ayymlssigp hmnkylwwkk ylttlqliqf siiflhnlqv ffngcnfpkp isfllclnal
      241 ifiymfssfy vetytkkrvs atkectngtv knkpe
//
LOCUS       XP_034191094             112 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein L55 [Osmia lignaria lignaria].
ACCESSION   XP_034191094
VERSION     XP_034191094.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335203.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..112
                     /product="mitochondrial ribosomal protein L55"
                     /calculated_mol_wt=13250
     Region          21..104
                     /region_name="Mitoc_L55"
                     /note="Mitochondrial ribosomal protein L55; pfam09776"
                     /db_xref="CDD:462893"
     CDS             1..112
                     /gene="mRpL55"
                     /coded_by="XM_034335203.2:134..472"
                     /db_xref="GeneID:117609190"
ORIGIN      
        1 mnissflrtt qisltlhrnl ncwtagitkk hrklyprdyl tylvlphggs irieydtprr
       61 iiclpldlet lteeqrkqrm earktytkak iveeyqdefd esqffnpskg rk
//
LOCUS       XP_034191095             614 aa            linear   INV 03-SEP-2025
DEFINITION  protein undicht [Osmia lignaria lignaria].
ACCESSION   XP_034191095
VERSION     XP_034191095.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335204.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..614
                     /product="protein undicht"
                     /calculated_mol_wt=71351
     CDS             1..614
                     /gene="udt"
                     /coded_by="XM_034335204.2:127..1971"
                     /db_xref="GeneID:117609193"
ORIGIN      
        1 mteklylflf aacfmwqvmp slqdssscqi pliirgswfs wengrntlte inaesmtnrg
       61 ycvnmleeyh vnytfvfqhd kcyhcvkliv rtvnvlekle vscvnlpdnv eptvknvckg
      121 lrpdqqlitl fsenyvpvnc rsslegvwqf ayqnrfrftg ecnhpdaqir scqtagtqfl
      181 itnqkfnity kqcpgmkdtf dgvveynclg dwfvdknhff avantkesrk dekyrcflkn
      241 rdddlyigvs itaecntlkt veksperlrv tpvkaevvep gcrlpedmsg qwintanida
      301 difinethii etwypdegry rrtiyvcrea kdtrvmmarl tvdgcqkdyv cfdfvrrhhn
      361 iiryrrgiav ikdnfptvcs wvqfpnkeaw kydlflaknp vpvrcpvagk ymfhqkgdvl
      421 fetrilggvt lsprpniyck qnisdfsvcd tdqkeiaide tyclsvdhlg rpvdiyslpd
      481 ykmkcigfwk enlksylith deldpfskyr cwvyqradln kvlmseaigp fcdlkqdvts
      541 snytegaava lelqeyerer drcpmyfddg snpwveteny iqvfhygsgg ikisfsypsl
      601 lliinvicir fiit
//
LOCUS       XP_034191096             355 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191096
VERSION     XP_034191096.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335205.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..355
                     /product="very long chain fatty acid elongase 7 isoform
                     X1"
                     /calculated_mol_wt=41382
     Region          55..292
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..355
                     /gene="LOC117609194"
                     /coded_by="XM_034335205.2:72..1139"
                     /db_xref="GeneID:117609194"
ORIGIN      
        1 mtftslniyt ceqkyskhfy sktrgkmtsi vadlvhgyrd lmdnksdprv nsyilmsspf
       61 ptmaiclfya yfskvlgprl menkkpfklr giligynliq tifstwifye ylmsgwakgy
      121 sfrcqpvdys hspmalrmvn tcwwyfiskf teffdtlffi lrkktqhvst lhvihhgimp
      181 fsvwlglkfa pgghstffal lntfvhiimy fyymvaamgp eyqkyiwwkk ylttlqmvqf
      241 vlimshqfql fftecdyprs fmiwiglhgv lflglfsdfy kakyvdkskl sakqssqngs
      301 sttgmcmpvl edestprqng vssyatiynk eynscysngt nngyvannnt ekkla
//
LOCUS       XP_034191097             329 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191097
VERSION     XP_034191097.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335206.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="very long chain fatty acid elongase 7 isoform
                     X2"
                     /calculated_mol_wt=38238
     Region          29..266
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..329
                     /gene="LOC117609194"
                     /coded_by="XM_034335206.2:1098..2087"
                     /db_xref="GeneID:117609194"
ORIGIN      
        1 mtsivadlvh gyrdlmdnks dprvnsyilm sspfptmaic lfyayfskvl gprlmenkkp
       61 fklrgiligy nliqtifstw ifyeylmsgw akgysfrcqp vdyshspmal rmvntcwwyf
      121 iskfteffdt lffilrkktq hvstlhvihh gimpfsvwlg lkfapgghst ffallntfvh
      181 iimyfyymva amgpeyqkyi wwkkylttlq mvqfvlimsh qfqlfftecd yprsfmiwig
      241 lhgvlflglf sdfykakyvd ksklsakqss qngssttgmc mpvledestp rqngvssyat
      301 iynkeynscy sngtnngyva nnntekkla
//
LOCUS       XP_034191098             329 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191098
VERSION     XP_034191098.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335207.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="very long chain fatty acid elongase 7 isoform
                     X2"
                     /calculated_mol_wt=38238
     Region          29..266
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..329
                     /gene="LOC117609194"
                     /coded_by="XM_034335207.2:960..1949"
                     /db_xref="GeneID:117609194"
ORIGIN      
        1 mtsivadlvh gyrdlmdnks dprvnsyilm sspfptmaic lfyayfskvl gprlmenkkp
       61 fklrgiligy nliqtifstw ifyeylmsgw akgysfrcqp vdyshspmal rmvntcwwyf
      121 iskfteffdt lffilrkktq hvstlhvihh gimpfsvwlg lkfapgghst ffallntfvh
      181 iimyfyymva amgpeyqkyi wwkkylttlq mvqfvlimsh qfqlfftecd yprsfmiwig
      241 lhgvlflglf sdfykakyvd ksklsakqss qngssttgmc mpvledestp rqngvssyat
      301 iynkeynscy sngtnngyva nnntekkla
//
LOCUS       XP_034191101             329 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191101
VERSION     XP_034191101.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335210.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="very long chain fatty acid elongase 7 isoform
                     X2"
                     /calculated_mol_wt=38238
     Region          29..266
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..329
                     /gene="LOC117609194"
                     /coded_by="XM_034335210.2:171..1160"
                     /db_xref="GeneID:117609194"
ORIGIN      
        1 mtsivadlvh gyrdlmdnks dprvnsyilm sspfptmaic lfyayfskvl gprlmenkkp
       61 fklrgiligy nliqtifstw ifyeylmsgw akgysfrcqp vdyshspmal rmvntcwwyf
      121 iskfteffdt lffilrkktq hvstlhvihh gimpfsvwlg lkfapgghst ffallntfvh
      181 iimyfyymva amgpeyqkyi wwkkylttlq mvqfvlimsh qfqlfftecd yprsfmiwig
      241 lhgvlflglf sdfykakyvd ksklsakqss qngssttgmc mpvledestp rqngvssyat
      301 iynkeynscy sngtnngyva nnntekkla
//
LOCUS       XP_034191100             329 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 7 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191100
VERSION     XP_034191100.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335209.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..329
                     /product="very long chain fatty acid elongase 7 isoform
                     X2"
                     /calculated_mol_wt=38238
     Region          29..266
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..329
                     /gene="LOC117609194"
                     /coded_by="XM_034335209.2:188..1177"
                     /db_xref="GeneID:117609194"
ORIGIN      
        1 mtsivadlvh gyrdlmdnks dprvnsyilm sspfptmaic lfyayfskvl gprlmenkkp
       61 fklrgiligy nliqtifstw ifyeylmsgw akgysfrcqp vdyshspmal rmvntcwwyf
      121 iskfteffdt lffilrkktq hvstlhvihh gimpfsvwlg lkfapgghst ffallntfvh
      181 iimyfyymva amgpeyqkyi wwkkylttlq mvqfvlimsh qfqlfftecd yprsfmiwig
      241 lhgvlflglf sdfykakyvd ksklsakqss qngssttgmc mpvledestp rqngvssyat
      301 iynkeynscy sngtnngyva nnntekkla
//
LOCUS       XP_034191102             325 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191102
VERSION     XP_034191102.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335211.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..325
                     /product="very long chain fatty acid elongase AAEL008004"
                     /calculated_mol_wt=38819
     Region          28..263
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..325
                     /gene="LOC117609196"
                     /coded_by="XM_034335211.2:229..1206"
                     /db_xref="GeneID:117609196"
ORIGIN      
        1 maqivrtiyd gyrdlmdnks dprvndwflm sspfptlgic lfyvyfvkil gpklmenrkp
       61 fdlrrvmify nlfqvifsaw lfyesvasgw gghysfrcqp vdysnnplai rmvhgcwwyy
      121 fskftefmdt iffvlrkknn histlhvihh gcmpmsvwfg vkftpgghst ffgllntfvh
      181 ivmysyylla algpwiqpyl wwkkyltalq mvqfvlvmih afqllfidcn ypkafvwwig
      241 mhafmfyflf rdfyieayrk rkstvqlkkk eeeerkqren ngsvrrndak engaiyanqy
      301 kmatgyisdn glrnrvfidn rgfke
//
LOCUS       XP_034191103             321 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191103
VERSION     XP_034191103.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335212.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="very long chain fatty acid elongase AAEL008004
                     isoform X1"
                     /calculated_mol_wt=37802
     Region          27..264
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..321
                     /gene="LOC117609197"
                     /coded_by="XM_034335212.2:595..1560"
                     /db_xref="GeneID:117609197"
ORIGIN      
        1 msivmqyvdr lheildkhad qrttnwflms spfptlficl syvyvvkvlg pkwmenrkpf
       61 qlknaliayn lfqmlfsawl fyeiglsgwl tgdyslrcqp vdysdrpqtm rmvhacwwyy
      121 fskftefmdt iffvlrkknd hvstlhvihh gcmpmsvwfg vkftpgghst ffgllntfvh
      181 ivmytyylla amgprvqpyl wwkkyltvfq miqfvvvmih afqllfincn ypkafvwlig
      241 lhatmffflf nefyqqsyqq rrkplkrnqq nhhangvsng tisngisnts sgrrdaadyy
      301 vkgdslaase lnlrksyttt e
//
LOCUS       XP_034191105             321 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191105
VERSION     XP_034191105.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335214.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="very long chain fatty acid elongase AAEL008004
                     isoform X1"
                     /calculated_mol_wt=37802
     Region          27..264
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..321
                     /gene="LOC117609197"
                     /coded_by="XM_034335214.2:461..1426"
                     /db_xref="GeneID:117609197"
ORIGIN      
        1 msivmqyvdr lheildkhad qrttnwflms spfptlficl syvyvvkvlg pkwmenrkpf
       61 qlknaliayn lfqmlfsawl fyeiglsgwl tgdyslrcqp vdysdrpqtm rmvhacwwyy
      121 fskftefmdt iffvlrkknd hvstlhvihh gcmpmsvwfg vkftpgghst ffgllntfvh
      181 ivmytyylla amgprvqpyl wwkkyltvfq miqfvvvmih afqllfincn ypkafvwlig
      241 lhatmffflf nefyqqsyqq rrkplkrnqq nhhangvsng tisngisnts sgrrdaadyy
      301 vkgdslaase lnlrksyttt e
//
LOCUS       XP_034191106             321 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191106
VERSION     XP_034191106.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335215.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="very long chain fatty acid elongase AAEL008004
                     isoform X1"
                     /calculated_mol_wt=37802
     Region          27..264
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..321
                     /gene="LOC117609197"
                     /coded_by="XM_034335215.2:607..1572"
                     /db_xref="GeneID:117609197"
ORIGIN      
        1 msivmqyvdr lheildkhad qrttnwflms spfptlficl syvyvvkvlg pkwmenrkpf
       61 qlknaliayn lfqmlfsawl fyeiglsgwl tgdyslrcqp vdysdrpqtm rmvhacwwyy
      121 fskftefmdt iffvlrkknd hvstlhvihh gcmpmsvwfg vkftpgghst ffgllntfvh
      181 ivmytyylla amgprvqpyl wwkkyltvfq miqfvvvmih afqllfincn ypkafvwlig
      241 lhatmffflf nefyqqsyqq rrkplkrnqq nhhangvsng tisngisnts sgrrdaadyy
      301 vkgdslaase lnlrksyttt e
//
LOCUS       XP_034191107             320 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191107
VERSION     XP_034191107.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335216.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..320
                     /product="very long chain fatty acid elongase AAEL008004
                     isoform X2"
                     /calculated_mol_wt=37732
     Region          27..263
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..320
                     /gene="LOC117609197"
                     /coded_by="XM_034335216.2:603..1565"
                     /db_xref="GeneID:117609197"
ORIGIN      
        1 msivmqyvdr lheildkhad qrttnwflms spfptlficl syvyvvkvlg pkwmenrkpf
       61 qlknaliayn lfqmlfsawl fyeclmggww ghysfrcqpv dysnnptavr mvhacwwyyf
      121 skftefmdti ffvlrkkndh vstlhvihhg cmpmsvwfgv kftpgghstf fgllntfvhi
      181 vmytyyllaa mgprvqpylw wkkyltvfqm iqfvvvmiha fqllfincny pkafvwligl
      241 hatmffflfn efyqqsyqqr rkplkrnqqn hhangvsngt isngisntss grrdaadyyv
      301 kgdslaasel nlrksyttte
//
LOCUS       XP_034191108             293 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191108
VERSION     XP_034191108.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335217.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="very long chain fatty acid elongase AAEL008004
                     isoform X3"
                     /calculated_mol_wt=34605
     Region          27..236
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..293
                     /gene="LOC117609197"
                     /coded_by="XM_034335217.2:600..1481"
                     /db_xref="GeneID:117609197"
ORIGIN      
        1 msivmqyvdr lheildkhad qrttnwflms spfptlficl syvyvvkvlg pkwmenrkpf
       61 qlknaliayn lfqmlfsawl fyemvhacww yyfskftefm dtiffvlrkk ndhvstlhvi
      121 hhgcmpmsvw fgvkftpggh stffgllntf vhivmytyyl laamgprvqp ylwwkkyltv
      181 fqmiqfvvvm ihafqllfin cnypkafvwl iglhatmfff lfnefyqqsy qqrrkplkrn
      241 qqnhhangvs ngtisngisn tssgrrdaad yyvkgdslaa selnlrksyt tte
//
LOCUS       XP_034191109             304 aa            linear   INV 03-SEP-2025
DEFINITION  stuck in traffic [Osmia lignaria lignaria].
ACCESSION   XP_034191109
VERSION     XP_034191109.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335218.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="stuck in traffic"
                     /calculated_mol_wt=35872
     Region          40..271
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..304
                     /gene="sit"
                     /coded_by="XM_034335218.2:154..1068"
                     /db_xref="GeneID:117609198"
ORIGIN      
        1 mnisstppid rmsniynnsv vrfyhfvfhd lsdprtkdwf litspvpgas iligylyfvl
       61 swgprhmehr kpyqlknilv fynflqvlls fwlfyeglda awlrkyswkc qpvdhsyspd
      121 alrvargvyi yflakisell dtiffvlrkk enqitflhly hhtvmpmvsw gatkyypggh
      181 gtfigvinsf vhiimytyyl laalvpqyqk ylwwkkyitt lqmgqfclaf lhscqlliyd
      241 cdypkwslil ilpnamffyf lfadfyshay rtgtrhfksv gkkenevvkk mtngnmqngk
      301 gktd
//
LOCUS       XP_034191110             304 aa            linear   INV 03-SEP-2025
DEFINITION  stuck in traffic [Osmia lignaria lignaria].
ACCESSION   XP_034191110
VERSION     XP_034191110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..304
                     /product="stuck in traffic"
                     /calculated_mol_wt=35872
     Region          40..271
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..304
                     /gene="sit"
                     /coded_by="XM_034335219.2:384..1298"
                     /db_xref="GeneID:117609198"
ORIGIN      
        1 mnisstppid rmsniynnsv vrfyhfvfhd lsdprtkdwf litspvpgas iligylyfvl
       61 swgprhmehr kpyqlknilv fynflqvlls fwlfyeglda awlrkyswkc qpvdhsyspd
      121 alrvargvyi yflakisell dtiffvlrkk enqitflhly hhtvmpmvsw gatkyypggh
      181 gtfigvinsf vhiimytyyl laalvpqyqk ylwwkkyitt lqmgqfclaf lhscqlliyd
      241 cdypkwslil ilpnamffyf lfadfyshay rtgtrhfksv gkkenevvkk mtngnmqngk
      301 gktd
//
LOCUS       XP_034191111             299 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191111
VERSION     XP_034191111.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335220.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..299
                     /product="very long chain fatty acid elongase AAEL008004"
                     /calculated_mol_wt=34571
     Region          57..295
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..299
                     /gene="LOC117609199"
                     /coded_by="XM_034335220.2:568..1467"
                     /db_xref="GeneID:117609199"
ORIGIN      
        1 masaaaelva erepepriem tdtnrtfvga qglvrmaldq yteilatisd prvsdwplmd
       61 spiptlfivi lylygvtifg prvmtnrkpf klrealvayn afqvvfslgm lyehlmsgwl
      121 ldysykcqpv dyshnpsalr manlcwwyfi skftefadti ffilrkkdsq vtflhlyhhs
      181 ltpletwicv kfipgghgtl gnlinnavhv imytyymvsa mgpeyqkylw wkkhlttvql
      241 vqfflvfvhs tqalvfdcgy pkliaallll hstiffvlfs dfyrrayntv ttrkqikge
//
LOCUS       XP_034191112             299 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase AAEL008004 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191112
VERSION     XP_034191112.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335221.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..299
                     /product="very long chain fatty acid elongase AAEL008004"
                     /calculated_mol_wt=34571
     Region          57..295
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..299
                     /gene="LOC117609199"
                     /coded_by="XM_034335221.2:585..1484"
                     /db_xref="GeneID:117609199"
ORIGIN      
        1 masaaaelva erepepriem tdtnrtfvga qglvrmaldq yteilatisd prvsdwplmd
       61 spiptlfivi lylygvtifg prvmtnrkpf klrealvayn afqvvfslgm lyehlmsgwl
      121 ldysykcqpv dyshnpsalr manlcwwyfi skftefadti ffilrkkdsq vtflhlyhhs
      181 ltpletwicv kfipgghgtl gnlinnavhv imytyymvsa mgpeyqkylw wkkhlttvql
      241 vqfflvfvhs tqalvfdcgy pkliaallll hstiffvlfs dfyrrayntv ttrkqikge
//
LOCUS       XP_034191113             267 aa            linear   INV 03-SEP-2025
DEFINITION  elongation of very long chain fatty acid james bond protein [Osmia
            lignaria lignaria].
ACCESSION   XP_034191113
VERSION     XP_034191113.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335222.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191113.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..267
                     /product="elongation of very long chain fatty acid james
                     bond protein"
                     /calculated_mol_wt=31102
     Region          29..266
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..267
                     /gene="bond"
                     /coded_by="XM_034335222.2:198..1001"
                     /db_xref="GeneID:117609200"
ORIGIN      
        1 msnlmrlvvn nyheiletnk einvdswamm gspgpmlciv gtylafvlkv gpkmmekrpa
       61 fqlnslliiy naflvifsiw msavpaiypg lkrllfspkc snsnrvhrdt qveaalanla
      121 wwyfvakiie lldtiffvlr kkqnqvtflh vyhhtmtsvf swcylkflpg eqgailgfln
      181 stvhivmyay yliaalgpey rkylwwkkym tgiqltqfal mfgyltmilv mdcklpkalt
      241 yffitnvvif iylfsdfyrk aykkkql
//
LOCUS       XP_034191115             246 aa            linear   INV 03-SEP-2025
DEFINITION  small nuclear ribonucleoprotein polypeptide A'-like U2A [Osmia
            lignaria lignaria].
ACCESSION   XP_034191115
VERSION     XP_034191115.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335224.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..246
                     /product="small nuclear ribonucleoprotein polypeptide
                     A'-like U2A"
                     /calculated_mol_wt=28266
     Region          1..175
                     /region_name="LRR_9"
                     /note="Leucine-rich repeat; pfam14580"
                     /db_xref="CDD:405295"
     Region          22..43
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          44..65
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          66..89
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          90..114
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     Region          115..126
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275378"
     CDS             1..246
                     /gene="U2A"
                     /coded_by="XM_034335224.2:88..828"
                     /db_xref="GeneID:117609201"
ORIGIN      
        1 mvkltpdliq qsmqyinpvk dreldlrgyk iptienlgat ldqfdtidfs dndirkldgf
       61 pllkriktlf fnnnrivrig eglehcipnl etlmltgnmi qelgdleplt qlknltnlcl
      121 lqnpvsakpq yrqyvvyrfp qlrlldfrki kmkereaava yfrskrgkem vreiakkvkt
      181 qssgastdkp lttpeernki reaitnatsl eevqrlskll qaghmpseer lqngntmpea
      241 meeedd
//
LOCUS       XP_034191116             186 aa            linear   INV 03-SEP-2025
DEFINITION  ADP-ribosylation factor-like protein 8 [Osmia lignaria lignaria].
ACCESSION   XP_034191116
VERSION     XP_034191116.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335225.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..186
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..186
                     /product="ADP-ribosylation factor-like protein 8"
                     /calculated_mol_wt=21117
     Region          22..180
                     /region_name="Arl10_like"
                     /note="Arf-like 9 (Arl9) and 10 (Arl10) GTPases; cd04159"
                     /db_xref="CDD:206724"
     Site            27..34
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206724"
     Site            order(29..30,34,38,51..58,80,85)
                     /site_type="other"
                     /note="putative GAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206724"
     Site            order(30..35,130..131,133..134,163..165)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206724"
     Site            order(39..42,45..55)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206724"
     Site            order(52..56,61,71,75,81,83..85)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206724"
     Site            52
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206724"
     Site            order(53..60,70,81,84..85)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206724"
     Site            order(56..63,65..70)
                     /site_type="active"
                     /note="interswitch region [active]"
                     /db_xref="CDD:206724"
     Site            71..88
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206724"
     Site            71..74
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206724"
     Site            130..133
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206724"
     Site            163..165
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206724"
     CDS             1..186
                     /gene="Arl8"
                     /coded_by="XM_034335225.2:331..891"
                     /db_xref="GeneID:117609202"
ORIGIN      
        1 mlalinrild wfkslfwkee meltlvglqy sgkttfvnvi asgqfsedmi ptvgfnmrki
       61 tkgnvtikvw diggqprfrs mwerycrgvn aivymvdaad aekieasrne lhnlldkpql
      121 sgipvlvlgn krdlphalde ngliermnls aiqdreiccy sisckekdni ditlqwliah
      181 srsgvr
//
LOCUS       XP_034191117             123 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase (ubiquinone) B18 subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034191117
VERSION     XP_034191117.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335226.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="NADH dehydrogenase (ubiquinone) B18 subunit"
                     /calculated_mol_wt=14620
     Region          40..101
                     /region_name="NDUF_B7"
                     /note="NADH-ubiquinone oxidoreductase B18 subunit
                     (NDUFB7); pfam05676"
                     /db_xref="CDD:461710"
     CDS             1..123
                     /gene="ND-B18"
                     /coded_by="XM_034335226.2:147..518"
                     /db_xref="GeneID:117609203"
ORIGIN      
        1 mgntiytwah pgpypdtdgt ptfdpllgfp hgrkkrvmkv teqelmaakv pmrlrdycah
       61 hyleymecmg vhfpfflnce kyyhkhmhce yedyvlrlke yererrllvr hkkkqeaiqa
      121 aaa
//
LOCUS       XP_034191118             113 aa            linear   INV 03-SEP-2025
DEFINITION  protein FAM32A [Osmia lignaria lignaria].
ACCESSION   XP_034191118
VERSION     XP_034191118.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335227.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..113
                     /product="protein FAM32A"
                     /calculated_mol_wt=13063
     CDS             1..113
                     /gene="LOC117609204"
                     /coded_by="XM_034335227.2:54..395"
                     /db_xref="GeneID:117609204"
ORIGIN      
        1 mpsandedpy ahvakgplkl ksdqcvskkk knkegkkkkl vevtkvleee knntvevkrt
       61 kaelafqtmq ekmqterikq kasmthkqrv eefnrhldsl tehfdipkvs wtk
//
LOCUS       XP_034191119            1342 aa            linear   INV 03-SEP-2025
DEFINITION  son of sevenless isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191119
VERSION     XP_034191119.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335228.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1342
                     /product="son of sevenless isoform X1"
                     /calculated_mol_wt=151203
     Region          35..113
                     /region_name="HFD_SOS1_rpt1"
                     /note="first histone-fold domain found in son of sevenless
                     homolog 1 (SOS-1) and similar proteins; cd22914"
                     /db_xref="CDD:467039"
     Region          122..196
                     /region_name="HFD_SOS1_rpt2"
                     /note="second histone-fold domain found in son of
                     sevenless homolog 1 (SOS-1) and similar proteins; cd22915"
                     /db_xref="CDD:467040"
     Region          220..403
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; smart00325"
                     /db_xref="CDD:214619"
     Site            order(222,226,324,352..353,356..357,359..360,363..364,
                     367..368,371,400)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          445..564
                     /region_name="PH_SOS"
                     /note="Son of Sevenless (SOS) Pleckstrin homology (PH)
                     domain; cd01261"
                     /db_xref="CDD:269963"
     Region          614..757
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(633,700,704..705,708,711..712,745..746,749,756)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          790..1030
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(823..825,835..836,838..840,842..843,846..847,850,
                     883,886..887,889..891,893..896,898..899,919,926..928,931,
                     944..946,949..951,953..955,957..960,977,981,1022,
                     1025..1026)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          <1032..1161
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1342
                     /gene="Sos"
                     /coded_by="XM_034335228.2:368..4396"
                     /db_xref="GeneID:117609205"
ORIGIN      
        1 mrdslcqyve mfsspasvvg epsgydfqke enaakwkgvf vnslskvleq vhpslearqd
       61 aldyveslil rllgmlcghp pphtpqdvee rvrrtfptpi drwalrdare alekgkkksp
      121 lvlpvdkihh llqkevlqqk idlqvslfvv gvleyisadi lklvgnyvkn irhveisced
      181 irvamcadmv lmdlfhqgdy aegpqsglga vvsqtyeesv rdlihderhy lrdlhmiikv
      241 freeiakltq drteletlfs nimdiyevtv tvlgsledim eiteekqtpt fgscfedlae
      301 aaeldvyiky akdinspasr evlanllsrp eanaalraag hgfkeavkyy lpklllqpiw
      361 hcflyfdyik vlhertpnge dgetllqaqg ilaplkkell qsvaslpkkd tglrmqsrar
      421 rqaalektse lqktvdgwdq rdvgqccnef iredmlgkvs ngrrlterka llfdgllvlc
      481 kpsggkrvsv tvaavgvvgh pphqgelrlk erffirkvdi idredteelk nafeiaprdh
      541 pnvilvaksv edknswmadl vmlntksmle rtldsillde erkhplrlpp phlykfaeqd
      601 spenivlear enggvplikg ailvklverl tyhiyadpaf vrtflttyrs fcspqelltl
      661 lierfdipdp slvygeeekp sgckttared wkryrkefcq pvqfrvlnvl rhwvdhhfyd
      721 ferdrnller lqsfldtvsg ksmrkwvdsv ikivqrkcep seqrpitfsf erspppiewh
      781 lkvpeeewgi ltlhpielar qltllefely rtvkpselvg svwtkrdkek tspnllkmik
      841 httnftrwie kniveaenle ervaivsrai eimmvlqdln nfngvlaivs amgsasvfrl
      901 kftfqqlpar lekaleeare lnnngrfrky qeklrsinpp cvpffgmylt nilhieegns
      961 dylpgspeli nfskrrkvae itgeiqqyqn qpyclsvepr irhfienlsp fdpnmkeadi
     1021 snylynksle veprgcrqpp rvprkwpdln lkspgikars lsgklpaplq avassvrlhd
     1081 pppevppqel petpphtshi tvshvggdhs vfapvllggp vqppgspgpl smsgfsissp
     1141 gsspqlvspg hlpvphhqpq sshfgfnsgt igvmgaltgi ssssgspgas mppppspshv
     1201 psnqpppplp prshrkress isespqqarq apnapilppr dntfppplpp rrdlppvtlp
     1261 prlpatlnpn csallarrns tlentcssav htrrhmsfng psptklpptq pngsvtprlp
     1321 pkplpgrppt tmfnfnappg ss
//
LOCUS       XP_076548170            1158 aa            linear   INV 03-SEP-2025
DEFINITION  son of sevenless isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548170
VERSION     XP_076548170.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692055.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1158
                     /product="son of sevenless isoform X2"
                     /calculated_mol_wt=130296
     Region          36..219
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; smart00325"
                     /db_xref="CDD:214619"
     Site            order(38,42,140,168..169,172..173,175..176,179..180,
                     183..184,187,216)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          261..380
                     /region_name="PH_SOS"
                     /note="Son of Sevenless (SOS) Pleckstrin homology (PH)
                     domain; cd01261"
                     /db_xref="CDD:269963"
     Region          430..573
                     /region_name="RasGEFN"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     GTPases; N-terminal motif; smart00229"
                     /db_xref="CDD:214571"
     Site            order(449,516,520..521,524,527..528,561..562,565,572)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:100121"
     Region          606..846
                     /region_name="RasGEF"
                     /note="Guanine nucleotide exchange factor for Ras-like
                     small GTPases. Small GTP-binding proteins of the Ras
                     superfamily function as molecular switches in fundamental
                     events such as signal transduction, cytoskeleton dynamics
                     and intracellular trafficking; cd00155"
                     /db_xref="CDD:238087"
     Site            order(639..641,651..652,654..656,658..659,662..663,666,
                     699,702..703,705..707,709..712,714..715,735,742..744,747,
                     760..762,765..767,769..771,773..776,793,797,838,841..842)
                     /site_type="other"
                     /note="Ras interaction site [polypeptide binding]"
                     /db_xref="CDD:238087"
     Region          <848..977
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..1158
                     /gene="Sos"
                     /coded_by="XM_076692055.1:113..3589"
                     /db_xref="GeneID:117609205"
ORIGIN      
        1 mcadmvlmdl fhqgdyaegp qsglgavvsq tyeesvrdli hderhylrdl hmiikvfree
       61 iakltqdrte letlfsnimd iyevtvtvlg sledimeite ekqtptfgsc fedlaeaael
      121 dvyikyakdi nspasrevla nllsrpeana alraaghgfk eavkyylpkl llqpiwhcfl
      181 yfdyikvlhe rtpngedget llqaqgilap lkkellqsva slpkkdtglr mqsrarrqaa
      241 lektselqkt vdgwdqrdvg qccnefired mlgkvsngrr lterkallfd gllvlckpsg
      301 gkrvsvtvaa vgvvghpphq gelrlkerff irkvdiidre dteelknafe iaprdhpnvi
      361 lvaksvedkn swmadlvmln tksmlertld silldeerkh plrlppphly kfaeqdspen
      421 ivlearengg vplikgailv klverltyhi yadpafvrtf lttyrsfcsp qelltllier
      481 fdipdpslvy geeekpsgck ttaredwkry rkefcqpvqf rvlnvlrhwv dhhfydferd
      541 rnllerlqsf ldtvsgksmr kwvdsvikiv qrkcepseqr pitfsfersp ppiewhlkvp
      601 eeewgiltlh pielarqltl lefelyrtvk pselvgsvwt krdkektspn llkmikhttn
      661 ftrwiekniv eaenleerva ivsraieimm vlqdlnnfng vlaivsamgs asvfrlkftf
      721 qqlparleka leearelnnn grfrkyqekl rsinppcvpf fgmyltnilh ieegnsdylp
      781 gspelinfsk rrkvaeitge iqqyqnqpyc lsveprirhf ienlspfdpn mkeadisnyl
      841 ynkslevepr gcrqpprvpr kwpdlnlksp gikarslsgk lpaplqavas svrlhdpppe
      901 vppqelpetp phtshitvsh vggdhsvfap vllggpvqpp gspgplsmsg fsisspgssp
      961 qlvspghlpv phhqpqsshf gfnsgtigvm galtgissss gspgasmppp pspshvpsnq
     1021 pppplpprsh rkressises pqqarqapna pilpprdntf ppplpprrdl ppvtlpprlp
     1081 atlnpncsal larrnstlen tcssavhtrr hmsfngpspt klpptqpngs vtprlppkpl
     1141 pgrppttmfn fnappgss
//
LOCUS       XP_034191121            1125 aa            linear   INV 03-SEP-2025
DEFINITION  myelin regulatory factor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191121
VERSION     XP_034191121.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335230.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1125
                     /product="myelin regulatory factor isoform X1"
                     /calculated_mol_wt=124952
     Region          329..481
                     /region_name="NDT80_PhoG"
                     /note="NDT80 / PhoG like DNA-binding family; pfam05224"
                     /db_xref="CDD:398753"
     Region          533..652
                     /region_name="Peptidase_S74_CIMCD"
                     /note="Peptidase S74 family, C-terminal intramolecular
                     chaperone domain of Escherichia coli phage K1F
                     endosialidase and related proteins; cd10144"
                     /db_xref="CDD:381748"
     Site            order(538..545,549,553..554,556,558..563,568,571,577..586,
                     600..606,611,613,632..635,637..640,642..646,648..649)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:381748"
     Region          986..1123
                     /region_name="MRF_C2"
                     /note="Myelin gene regulatory factor C-terminal domain 2;
                     pfam13888"
                     /db_xref="CDD:464020"
     CDS             1..1125
                     /gene="LOC117609206"
                     /coded_by="XM_034335230.2:545..3922"
                     /db_xref="GeneID:117609206"
ORIGIN      
        1 mdvigdgdeq tlqailgrdd fdggldfsql edfinsdseq patyfadtla hnengggpts
       61 hggrveattt qqplsrlpsp iaqshpvsst ctsgtttvpn gatykdpqsy vhphalpesp
      121 pdsgseppys ppghndtqhv hsphqkvalq emllhhqgnq anyapnmlps sprplpsstd
      181 pmllthsvlt shlgpgtpni qpqqqitqnl vplphehspg gintlysslq sapkkrklsq
      241 dglvhvkqvq repelgtveh scssssavld gdevadnsyi dasyqcirfh pfqqtswhvl
      301 cdhnlkelpv phyrvdadkg fnfsnsddaf vcqkknhfqi tchaqlqgea ifvrtgeglk
      361 kissfqlhfy gvkvesptqt irveqsqsdr skkpfhpvta afrvelgger vtkvtvgrlh
      421 fsettsnnmr kkgkpnpdqr yfhlvvglha htadqasyqv vahaseriiv rasnpgqfes
      481 egsgvgaegg wqrgsapdsv yhagrvgint drpdealvvh gnmkvtghiv qpsdarakqn
      541 vqeidtreql rnvqqlrvvr yryapefaqh sglgikqqed tgviaqevqq ilpeavlpag
      601 divlpngqri enflvvnker ifmenvgavk elckvtdsle tridqlerin krlaklkrdd
      661 slkssistis skttvqgkgk kneredeflc snkfiqiviv ililimafcl vamatlyfle
      721 yqkrssfewp avssngmlai rpahpstiss tpnydpryns llgstlssly tkhgshsrgl
      781 dsslsvksda fstsaprtkq dyysheitwf hstkleknse fpgnwlsrng agvvpsnvde
      841 ndqgsdvess tlnkgpiplg rpvdcprhfs efgnpckvhc cttkmhqfee pqpdhppekk
      901 sipdhieqpl nvheekrkit kgfqnginps dpstqtlvke knyihsykrt rretnggdwv
      961 evasnaagsl ppepkpqlwi vaenfntsld qkycsassqd tpnnitciip lskympdvrl
     1021 tlhfvgmpwy gqvvqqctsl tnpgidesmi cspkdaiqqq telkidiesn nqrgdqsfpl
     1081 dvahylrktl rfrvptvqpq enicknkhgv dyseytlhfy rdcde
//
LOCUS       XP_034191123            1124 aa            linear   INV 03-SEP-2025
DEFINITION  myelin regulatory factor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191123
VERSION     XP_034191123.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335232.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1124
                     /product="myelin regulatory factor isoform X2"
                     /calculated_mol_wt=124877
     Region          329..481
                     /region_name="NDT80_PhoG"
                     /note="NDT80 / PhoG like DNA-binding family; pfam05224"
                     /db_xref="CDD:398753"
     Region          533..652
                     /region_name="Peptidase_S74_CIMCD"
                     /note="Peptidase S74 family, C-terminal intramolecular
                     chaperone domain of Escherichia coli phage K1F
                     endosialidase and related proteins; cd10144"
                     /db_xref="CDD:381748"
     Site            order(538..545,549,553..554,556,558..563,568,571,577..586,
                     600..606,611,613,632..635,637..640,642..646,648..649)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:381748"
     Region          985..1122
                     /region_name="MRF_C2"
                     /note="Myelin gene regulatory factor C-terminal domain 2;
                     pfam13888"
                     /db_xref="CDD:464020"
     CDS             1..1124
                     /gene="LOC117609206"
                     /coded_by="XM_034335232.2:545..3919"
                     /db_xref="GeneID:117609206"
ORIGIN      
        1 mdvigdgdeq tlqailgrdd fdggldfsql edfinsdseq patyfadtla hnengggpts
       61 hggrveattt qqplsrlpsp iaqshpvsst ctsgtttvpn gatykdpqsy vhphalpesp
      121 pdsgseppys ppghndtqhv hsphqkvalq emllhhqgnq anyapnmlps sprplpsstd
      181 pmllthsvlt shlgpgtpni qpqqqitqnl vplphehspg gintlysslq sapkkrklsq
      241 dglvhvkqvq repelgtveh scssssavld gdevadnsyi dasyqcirfh pfqqtswhvl
      301 cdhnlkelpv phyrvdadkg fnfsnsddaf vcqkknhfqi tchaqlqgea ifvrtgeglk
      361 kissfqlhfy gvkvesptqt irveqsqsdr skkpfhpvta afrvelgger vtkvtvgrlh
      421 fsettsnnmr kkgkpnpdqr yfhlvvglha htadqasyqv vahaseriiv rasnpgqfes
      481 egsgvgaegg wqrgsapdsv yhagrvgint drpdealvvh gnmkvtghiv qpsdarakqn
      541 vqeidtreql rnvqqlrvvr yryapefaqh sglgikqqed tgviaqevqq ilpeavlpag
      601 divlpngqri enflvvnker ifmenvgavk elckvtdsle tridqlerin krlaklkrdd
      661 slkssistis skttvqgkgk kneredeflc snkfiqiviv ililimafcl vamatlyfle
      721 yqkrssfewp avssngmlai rpahpstiss tpnydpryns llgstlssly tkhgshsrgl
      781 dsslsvksda fstsaprtkq dyysheitwf hstkleknse fpgnwlsrng agvvpsnvde
      841 ndqgsdvess tlnkgpiplg rpvdcprhfs efgnpckvhc cttkmhqfee pqpdhppekk
      901 sihhieqpln vheekrkitk gfqnginpsd pstqtlvkek nyihsykrtr retnggdwve
      961 vasnaagslp pepkpqlwiv aenfntsldq kycsassqdt pnnitciipl skympdvrlt
     1021 lhfvgmpwyg qvvqqctslt npgidesmic spkdaiqqqt elkidiesnn qrgdqsfpld
     1081 vahylrktlr frvptvqpqe nicknkhgvd yseytlhfyr dcde
//
LOCUS       XP_034191124            1122 aa            linear   INV 03-SEP-2025
DEFINITION  myelin regulatory factor isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191124
VERSION     XP_034191124.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335233.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1122
                     /product="myelin regulatory factor isoform X3"
                     /calculated_mol_wt=124569
     Region          326..478
                     /region_name="NDT80_PhoG"
                     /note="NDT80 / PhoG like DNA-binding family; pfam05224"
                     /db_xref="CDD:398753"
     Region          530..649
                     /region_name="Peptidase_S74_CIMCD"
                     /note="Peptidase S74 family, C-terminal intramolecular
                     chaperone domain of Escherichia coli phage K1F
                     endosialidase and related proteins; cd10144"
                     /db_xref="CDD:381748"
     Site            order(535..542,546,550..551,553,555..560,565,568,574..583,
                     597..603,608,610,629..632,634..637,639..643,645..646)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:381748"
     Region          983..1120
                     /region_name="MRF_C2"
                     /note="Myelin gene regulatory factor C-terminal domain 2;
                     pfam13888"
                     /db_xref="CDD:464020"
     CDS             1..1122
                     /gene="LOC117609206"
                     /coded_by="XM_034335233.2:544..3912"
                     /db_xref="GeneID:117609206"
ORIGIN      
        1 mdvigdgdeq tlqailgrdd fdggldfsql edfinsdseq patyfadtla hnengggpts
       61 hggrveattt qqplsrlpsp iaqshpvsst ctsgtttvpn gatykdpqsy vhphalpesp
      121 pdsgseppys ppghndtqhv hsphqkvalq emllhhqgnq anyapnmlps sprplpsstd
      181 pmllthsvlt shlgpgtpni qpqqqitqnl vplphehspg gintlysslq sapkkrklsq
      241 dglvhvkqep elgtvehscs sssavldgde vadnsyidas yqcirfhpfq qtswhvlcdh
      301 nlkelpvphy rvdadkgfnf snsddafvcq kknhfqitch aqlqgeaifv rtgeglkkis
      361 sfqlhfygvk vesptqtirv eqsqsdrskk pfhpvtaafr velggervtk vtvgrlhfse
      421 ttsnnmrkkg kpnpdqryfh lvvglhahta dqasyqvvah aseriivras npgqfesegs
      481 gvgaeggwqr gsapdsvyha grvgintdrp dealvvhgnm kvtghivqps darakqnvqe
      541 idtreqlrnv qqlrvvryry apefaqhsgl gikqqedtgv iaqevqqilp eavlpagdiv
      601 lpngqrienf lvvnkerifm envgavkelc kvtdsletri dqlerinkrl aklkrddslk
      661 ssistisskt tvqgkgkkne redeflcsnk fiqivivili limafclvam atlyfleyqk
      721 rssfewpavs sngmlairpa hpstisstpn ydprynsllg stlsslytkh gshsrgldss
      781 lsvksdafst saprtkqdyy sheitwfhst kleknsefpg nwlsrngagv vpsnvdendq
      841 gsdvesstln kgpiplgrpv dcprhfsefg npckvhcctt kmhqfeepqp dhppekksip
      901 dhieqplnvh eekrkitkgf qnginpsdps tqtlvkekny ihsykrtrre tnggdwveva
      961 snaagslppe pkpqlwivae nfntsldqky csassqdtpn nitciiplsk ympdvrltlh
     1021 fvgmpwygqv vqqctsltnp gidesmicsp kdaiqqqtel kidiesnnqr gdqsfpldva
     1081 hylrktlrfr vptvqpqeni cknkhgvdys eytlhfyrdc de
//
LOCUS       XP_034191125            1121 aa            linear   INV 03-SEP-2025
DEFINITION  myelin regulatory factor isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034191125
VERSION     XP_034191125.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335234.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1121
                     /product="myelin regulatory factor isoform X4"
                     /calculated_mol_wt=124507
     Region          329..477
                     /region_name="NDT80_PhoG"
                     /note="NDT80 / PhoG like DNA-binding family; pfam05224"
                     /db_xref="CDD:398753"
     Region          529..648
                     /region_name="Peptidase_S74_CIMCD"
                     /note="Peptidase S74 family, C-terminal intramolecular
                     chaperone domain of Escherichia coli phage K1F
                     endosialidase and related proteins; cd10144"
                     /db_xref="CDD:381748"
     Site            order(534..541,545,549..550,552,554..559,564,567,573..582,
                     596..602,607,609,628..631,633..636,638..642,644..645)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:381748"
     Region          982..1119
                     /region_name="MRF_C2"
                     /note="Myelin gene regulatory factor C-terminal domain 2;
                     pfam13888"
                     /db_xref="CDD:464020"
     CDS             1..1121
                     /gene="LOC117609206"
                     /coded_by="XM_034335234.2:543..3908"
                     /db_xref="GeneID:117609206"
ORIGIN      
        1 mdvigdgdeq tlqailgrdd fdggldfsql edfinsdseq patyfadtla hnengggpts
       61 hggrveattt qqplsrlpsp iaqshpvsst ctsgtttvpn gatykdpqsy vhphalpesp
      121 pdsgseppys ppghndtqhv hsphqkvalq emllhhqgnq anyapnmlps sprplpsstd
      181 pmllthsvlt shlgpgtpni qpqqqitqnl vplphehspg gintlysslq sapkkrklsq
      241 dglvhvkqvq repelgtveh scssssavld gdevadnsyi dasyqcirfh pfqqtswhvl
      301 cdhnlkelpv phyrvdadkg fnfsnsddaf vcqkknhfqi tchaqlqgea ifvrtgeglk
      361 kissfqlhfy gvkvesptqt irveqsqsdr skkpfhpvtv elggervtkv tvgrlhfset
      421 tsnnmrkkgk pnpdqryfhl vvglhahtad qasyqvvaha seriivrasn pgqfesegsg
      481 vgaeggwqrg sapdsvyhag rvgintdrpd ealvvhgnmk vtghivqpsd arakqnvqei
      541 dtreqlrnvq qlrvvryrya pefaqhsglg ikqqedtgvi aqevqqilpe avlpagdivl
      601 pngqrienfl vvnkerifme nvgavkelck vtdsletrid qlerinkrla klkrddslks
      661 sistissktt vqgkgkkner edeflcsnkf iqivivilil imafclvama tlyfleyqkr
      721 ssfewpavss ngmlairpah pstisstpny dprynsllgs tlsslytkhg shsrgldssl
      781 svksdafsts aprtkqdyys heitwfhstk leknsefpgn wlsrngagvv psnvdendqg
      841 sdvesstlnk gpiplgrpvd cprhfsefgn pckvhccttk mhqfeepqpd hppekksipd
      901 hieqplnvhe ekrkitkgfq nginpsdpst qtlvkeknyi hsykrtrret nggdwvevas
      961 naagslppep kpqlwivaen fntsldqkyc sassqdtpnn itciiplsky mpdvrltlhf
     1021 vgmpwygqvv qqctsltnpg idesmicspk daiqqqtelk idiesnnqrg dqsfpldvah
     1081 ylrktlrfrv ptvqpqenic knkhgvdyse ytlhfyrdcd e
//
LOCUS       XP_034191126            1121 aa            linear   INV 03-SEP-2025
DEFINITION  myelin regulatory factor isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034191126
VERSION     XP_034191126.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335235.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1121
                     /product="myelin regulatory factor isoform X5"
                     /calculated_mol_wt=124456
     Region          325..477
                     /region_name="NDT80_PhoG"
                     /note="NDT80 / PhoG like DNA-binding family; pfam05224"
                     /db_xref="CDD:398753"
     Region          529..648
                     /region_name="Peptidase_S74_CIMCD"
                     /note="Peptidase S74 family, C-terminal intramolecular
                     chaperone domain of Escherichia coli phage K1F
                     endosialidase and related proteins; cd10144"
                     /db_xref="CDD:381748"
     Site            order(534..541,545,549..550,552,554..559,564,567,573..582,
                     596..602,607,609,628..631,633..636,638..642,644..645)
                     /site_type="other"
                     /note="trimer interface [polypeptide binding]"
                     /db_xref="CDD:381748"
     Region          982..1119
                     /region_name="MRF_C2"
                     /note="Myelin gene regulatory factor C-terminal domain 2;
                     pfam13888"
                     /db_xref="CDD:464020"
     CDS             1..1121
                     /gene="LOC117609206"
                     /coded_by="XM_034335235.2:545..3910"
                     /db_xref="GeneID:117609206"
ORIGIN      
        1 mdvigdgdeq tlqailgrdd fdggldfsql edfinsdseq patyfadtla hnengggpts
       61 hggrveattt qqplsrlpsp iaqshpvsst ctsgtttvpn gatykdpqsh alpesppdsg
      121 seppysppgh ndtqhvhsph qkvalqemll hhqgnqanya pnmlpssprp lpsstdpmll
      181 thsvltshlg pgtpniqpqq qitqnlvplp hehspggint lysslqsapk krklsqdglv
      241 hvkqvqrepe lgtvehscss ssavldgdev adnsyidasy qcirfhpfqq tswhvlcdhn
      301 lkelpvphyr vdadkgfnfs nsddafvcqk knhfqitcha qlqgeaifvr tgeglkkiss
      361 fqlhfygvkv esptqtirve qsqsdrskkp fhpvtaafrv elggervtkv tvgrlhfset
      421 tsnnmrkkgk pnpdqryfhl vvglhahtad qasyqvvaha seriivrasn pgqfesegsg
      481 vgaeggwqrg sapdsvyhag rvgintdrpd ealvvhgnmk vtghivqpsd arakqnvqei
      541 dtreqlrnvq qlrvvryrya pefaqhsglg ikqqedtgvi aqevqqilpe avlpagdivl
      601 pngqrienfl vvnkerifme nvgavkelck vtdsletrid qlerinkrla klkrddslks
      661 sistissktt vqgkgkkner edeflcsnkf iqivivilil imafclvama tlyfleyqkr
      721 ssfewpavss ngmlairpah pstisstpny dprynsllgs tlsslytkhg shsrgldssl
      781 svksdafsts aprtkqdyys heitwfhstk leknsefpgn wlsrngagvv psnvdendqg
      841 sdvesstlnk gpiplgrpvd cprhfsefgn pckvhccttk mhqfeepqpd hppekksipd
      901 hieqplnvhe ekrkitkgfq nginpsdpst qtlvkeknyi hsykrtrret nggdwvevas
      961 naagslppep kpqlwivaen fntsldqkyc sassqdtpnn itciiplsky mpdvrltlhf
     1021 vgmpwygqvv qqctsltnpg idesmicspk daiqqqtelk idiesnnqrg dqsfpldvah
     1081 ylrktlrfrv ptvqpqenic knkhgvdyse ytlhfyrdcd e
//
LOCUS       XP_034191129            1065 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin-4 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191129
VERSION     XP_034191129.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335238.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1065
                     /product="anoctamin-4 isoform X1"
                     /calculated_mol_wt=122456
     Region          168..403
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          406..867
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..1065
                     /gene="LOC117609207"
                     /coded_by="XM_034335238.2:1421..4618"
                     /db_xref="GeneID:117609207"
ORIGIN      
        1 mdnnelrved etqnnhsrrp sastnnttsf tdlspndsea naktenvssr vasacstpqk
       61 rrssmesnsp rvnkstqame nremiyetdk ngiqtpkanr klsrasvyps gtnltardaf
      121 gpkltapatp enspplldpp ifpddiprds llqkdsssea eidpetlyfr dgrrridmvl
      181 vyqednngvm teiearrkeq rrvfqqnllk eglqlelepk ensfdgktff lklhipwkik
      241 vqyaevmnlk lptkrfitis vkawdnentk krpklwdkwt rcirwiktlh twdtkkyate
      301 psfynsidsg dreerfvikd rdtaytpaqr slivmqillr arydethekv girrlladgs
      361 yldcyslheg pynkpglnge ildrhllyli warpsqwykk qplwlirryf gekvglyfaw
      421 lgfytkclyp paivgllcfm ygvgsmegad nipskeicds nvagnitlcp lcdkacdyqi
      481 lgescmfskl tylfdnpatv ffaifmsfwa ttflelwkrr qavimwewdl qnvesdeepr
      541 pefettvktf rinpvtrere pylptwskav rfcatgsmvf fmicvvlgav lgtiiyrisl
      601 vsvfysgggt flkkhakift smtaalvnli iimiltrvyh rlarwmvnme nprtqteyes
      661 sftfkiflfe fvnfyssliy iaffkgrffv hpgdadarts affriktdvc dpagclsevc
      721 iqlaiimvgk qcfnnfveil spklwnwwrk rnhiaatkdh arpytcwerd yqlqdpgrla
      781 lfdeylemil qygfvtlfva afplaplfal lnniaeirld aykmvkesrr plaervedig
      841 awygilrgvt yvavvsnafv iaytsdfipr svyaivyspt ndlvgyidss lsefntsdyr
      901 ddmksdmkek hpetcqyrgy rngpdhktdp yglspqywhv faarlafvvv fehvvfaltg
      961 imsyvipalp qalatqlare rllaqeakye kglksreded dilsmlreag sigraaaggr
     1021 gswarrfskl sdgldahvdv tsrqnrnsdg stvwenilsi rskac
//
LOCUS       XP_034191130            1065 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin-4 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191130
VERSION     XP_034191130.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335239.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1065
                     /product="anoctamin-4 isoform X1"
                     /calculated_mol_wt=122456
     Region          168..403
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          406..867
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..1065
                     /gene="LOC117609207"
                     /coded_by="XM_034335239.2:1871..5068"
                     /db_xref="GeneID:117609207"
ORIGIN      
        1 mdnnelrved etqnnhsrrp sastnnttsf tdlspndsea naktenvssr vasacstpqk
       61 rrssmesnsp rvnkstqame nremiyetdk ngiqtpkanr klsrasvyps gtnltardaf
      121 gpkltapatp enspplldpp ifpddiprds llqkdsssea eidpetlyfr dgrrridmvl
      181 vyqednngvm teiearrkeq rrvfqqnllk eglqlelepk ensfdgktff lklhipwkik
      241 vqyaevmnlk lptkrfitis vkawdnentk krpklwdkwt rcirwiktlh twdtkkyate
      301 psfynsidsg dreerfvikd rdtaytpaqr slivmqillr arydethekv girrlladgs
      361 yldcyslheg pynkpglnge ildrhllyli warpsqwykk qplwlirryf gekvglyfaw
      421 lgfytkclyp paivgllcfm ygvgsmegad nipskeicds nvagnitlcp lcdkacdyqi
      481 lgescmfskl tylfdnpatv ffaifmsfwa ttflelwkrr qavimwewdl qnvesdeepr
      541 pefettvktf rinpvtrere pylptwskav rfcatgsmvf fmicvvlgav lgtiiyrisl
      601 vsvfysgggt flkkhakift smtaalvnli iimiltrvyh rlarwmvnme nprtqteyes
      661 sftfkiflfe fvnfyssliy iaffkgrffv hpgdadarts affriktdvc dpagclsevc
      721 iqlaiimvgk qcfnnfveil spklwnwwrk rnhiaatkdh arpytcwerd yqlqdpgrla
      781 lfdeylemil qygfvtlfva afplaplfal lnniaeirld aykmvkesrr plaervedig
      841 awygilrgvt yvavvsnafv iaytsdfipr svyaivyspt ndlvgyidss lsefntsdyr
      901 ddmksdmkek hpetcqyrgy rngpdhktdp yglspqywhv faarlafvvv fehvvfaltg
      961 imsyvipalp qalatqlare rllaqeakye kglksreded dilsmlreag sigraaaggr
     1021 gswarrfskl sdgldahvdv tsrqnrnsdg stvwenilsi rskac
//
LOCUS       XP_034191131            1065 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin-4 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191131
VERSION     XP_034191131.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335240.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1065
                     /product="anoctamin-4 isoform X1"
                     /calculated_mol_wt=122456
     Region          168..403
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          406..867
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..1065
                     /gene="LOC117609207"
                     /coded_by="XM_034335240.2:1818..5015"
                     /db_xref="GeneID:117609207"
ORIGIN      
        1 mdnnelrved etqnnhsrrp sastnnttsf tdlspndsea naktenvssr vasacstpqk
       61 rrssmesnsp rvnkstqame nremiyetdk ngiqtpkanr klsrasvyps gtnltardaf
      121 gpkltapatp enspplldpp ifpddiprds llqkdsssea eidpetlyfr dgrrridmvl
      181 vyqednngvm teiearrkeq rrvfqqnllk eglqlelepk ensfdgktff lklhipwkik
      241 vqyaevmnlk lptkrfitis vkawdnentk krpklwdkwt rcirwiktlh twdtkkyate
      301 psfynsidsg dreerfvikd rdtaytpaqr slivmqillr arydethekv girrlladgs
      361 yldcyslheg pynkpglnge ildrhllyli warpsqwykk qplwlirryf gekvglyfaw
      421 lgfytkclyp paivgllcfm ygvgsmegad nipskeicds nvagnitlcp lcdkacdyqi
      481 lgescmfskl tylfdnpatv ffaifmsfwa ttflelwkrr qavimwewdl qnvesdeepr
      541 pefettvktf rinpvtrere pylptwskav rfcatgsmvf fmicvvlgav lgtiiyrisl
      601 vsvfysgggt flkkhakift smtaalvnli iimiltrvyh rlarwmvnme nprtqteyes
      661 sftfkiflfe fvnfyssliy iaffkgrffv hpgdadarts affriktdvc dpagclsevc
      721 iqlaiimvgk qcfnnfveil spklwnwwrk rnhiaatkdh arpytcwerd yqlqdpgrla
      781 lfdeylemil qygfvtlfva afplaplfal lnniaeirld aykmvkesrr plaervedig
      841 awygilrgvt yvavvsnafv iaytsdfipr svyaivyspt ndlvgyidss lsefntsdyr
      901 ddmksdmkek hpetcqyrgy rngpdhktdp yglspqywhv faarlafvvv fehvvfaltg
      961 imsyvipalp qalatqlare rllaqeakye kglksreded dilsmlreag sigraaaggr
     1021 gswarrfskl sdgldahvdv tsrqnrnsdg stvwenilsi rskac
//
LOCUS       XP_034191132            1061 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin-4 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191132
VERSION     XP_034191132.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335241.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1061
                     /product="anoctamin-4 isoform X2"
                     /calculated_mol_wt=122114
     Region          168..403
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          406..867
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..1061
                     /gene="LOC117609207"
                     /coded_by="XM_034335241.2:1881..5066"
                     /db_xref="GeneID:117609207"
ORIGIN      
        1 mdnnelrved etqnnhsrrp sastnnttsf tdlspndsea naktenvssr vasacstpqk
       61 rrssmesnsp rvnkstqame nremiyetdk ngiqtpkanr klsrasvyps gtnltardaf
      121 gpkltapatp enspplldpp ifpddiprds llqkdsssea eidpetlyfr dgrrridmvl
      181 vyqednngvm teiearrkeq rrvfqqnllk eglqlelepk ensfdgktff lklhipwkik
      241 vqyaevmnlk lptkrfitis vkawdnentk krpklwdkwt rcirwiktlh twdtkkyate
      301 psfynsidsg dreerfvikd rdtaytpaqr slivmqillr arydethekv girrlladgs
      361 yldcyslheg pynkpglnge ildrhllyli warpsqwykk qplwlirryf gekvglyfaw
      421 lgfytkclyp paivgllcfm ygvgsmegad nipskeicds nvagnitlcp lcdkacdyqi
      481 lgescmfskl tylfdnpatv ffaifmsfwa ttflelwkrr qavimwewdl qnvesdeepr
      541 pefettvktf rinpvtrere pylptwskav rfcatgsmvf fmicvvlgav lgtiiyrisl
      601 vsvfysgggt flkkhakift smtaalvnli iimiltrvyh rlarwmvnme nprtqteyes
      661 sftfkiflfe fvnfyssliy iaffkgrffv hpgdadarts affriktdvc dpagclsevc
      721 iqlaiimvgk qcfnnfveil spklwnwwrk rnhiaatkdh arpytcwerd yqlqdpgrla
      781 lfdeylemil qygfvtlfva afplaplfal lnniaeirld aykmvkesrr plaervedig
      841 awygilrgvt yvavvsnafv iaytsdfipr svyaivyspt ndlvgyidss lsefntsdyr
      901 ddmksdmkek hpetcqyrgy rngpdhktdp yglspqywhv faarlafvvv fehvvfaltg
      961 imsyvipalp qalatqlare rllaqeakye kglksreded dilsmlreag sigraaaggr
     1021 gswarrfskl sdgldahvdv tsrqnrnsdg stvweemaar w
//
LOCUS       XP_034191133            1040 aa            linear   INV 03-SEP-2025
DEFINITION  anoctamin-4 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191133
VERSION     XP_034191133.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335242.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1040
                     /product="anoctamin-4 isoform X3"
                     /calculated_mol_wt=119462
     Region          168..403
                     /region_name="Anoct_dimer"
                     /note="dimerization domain of Ca+-activated
                     chloride-channel, anoctamin; pfam16178"
                     /db_xref="CDD:465044"
     Region          406..842
                     /region_name="Anoctamin"
                     /note="Calcium-activated chloride channel; pfam04547"
                     /db_xref="CDD:461349"
     CDS             1..1040
                     /gene="LOC117609207"
                     /coded_by="XM_034335242.2:1881..5003"
                     /db_xref="GeneID:117609207"
ORIGIN      
        1 mdnnelrved etqnnhsrrp sastnnttsf tdlspndsea naktenvssr vasacstpqk
       61 rrssmesnsp rvnkstqame nremiyetdk ngiqtpkanr klsrasvyps gtnltardaf
      121 gpkltapatp enspplldpp ifpddiprds llqkdsssea eidpetlyfr dgrrridmvl
      181 vyqednngvm teiearrkeq rrvfqqnllk eglqlelepk ensfdgktff lklhipwkik
      241 vqyaevmnlk lptkrfitis vkawdnentk krpklwdkwt rcirwiktlh twdtkkyate
      301 psfynsidsg dreerfvikd rdtaytpaqr slivmqillr arydethekv girrlladgs
      361 yldcyslheg pynkpglnge ildrhllyli warpsqwykk qplwlirryf gekvglyfaw
      421 lgfytkclyp paivgllcfm ygvgsmegad nipskeicds nvagnitlcp lcdkacdyqi
      481 lgessttfle lwkrrqavim wewdlqnves deeprpefet tvktfrinpv trerepylpt
      541 wskavrfcat gsmvffmicv vlgavlgtii yrislvsvfy sgggtflkkh akiftsmtaa
      601 lvnliiimil trvyhrlarw mvnmenprtq teyessftfk iflfefvnfy ssliyiaffk
      661 grffvhpgda dartsaffri ktdvcdpagc lsevciqlai imvgkqcfnn fveilspklw
      721 nwwrkrnhia atkdharpyt cwerdyqlqd pgrlalfdey lemilqygfv tlfvaafpla
      781 plfallnnia eirldaykmv kesrrplaer vedigawygi lrgvtyvavv snafviayts
      841 dfiprsvyai vysptndlvg yidsslsefn tsdyrddmks dmkekhpetc qyrgyrngpd
      901 hktdpyglsp qywhvfaarl afvvvfehvv faltgimsyv ipalpqalat qlarerllaq
      961 eakyekglks rededdilsm lreagsigra aaggrgswar rfsklsdgld ahvdvtsrqn
     1021 rnsdgstvwe nilsirskac
//
LOCUS       XP_034191134            1010 aa            linear   INV 03-SEP-2025
DEFINITION  nucleosome-remodeling ATPase imitation SWI [Osmia lignaria
            lignaria].
ACCESSION   XP_034191134
VERSION     XP_034191134.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335243.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1010
                     /product="nucleosome-remodeling ATPase imitation SWI"
                     /calculated_mol_wt=117374
     Region          43..1010
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     CDS             1..1010
                     /gene="Iswi"
                     /coded_by="XM_034335243.2:323..3355"
                     /db_xref="GeneID:117609209"
ORIGIN      
        1 mskpdenadt gdtgdnsngs saettssrgg dfetkletdr skrfdyllkq teifshfmtn
       61 nqkdkagspl kikagrprkq pqepqiktds gdhrhrkteq eedeellaes nasvapttrf
      121 essphyiksg elrdyqirgl nwmislyeng ingilademg lgktlqtisl lgymkhfrni
      181 pgphivivpk stlanwmnef kkwcptlrav cligdaetrn tfirdvmmpg ewdvcvtsye
      241 mvikeksvfk kfnwrymvid eahrikneks klseilrefk tanrllltgt plqnnlhelw
      301 sllnfllpdv fnssddfdsw fntnsflgdn slverlhavl rpfllrrlks evekglkpkk
      361 eikvyiglsk mqrewytkvl mkdidivnga gkiekmrlqn ilmqlrkccn hpylfdgaep
      421 gppyttdehl vyncgkmvil dkllpklqqq esrvlifsqm trmldiledy chwrcfqycr
      481 ldgntahedr qrqineynap esekfifmls tragglginl atadvviiyd sdwnpqmdlq
      541 amdrahrigq qkqvrvfrfi tentveekiv eraevklrld klviqqgrlv dakqtalnkd
      601 emlnmirhga nevfaskdsa itdedidtil qkgeakteem kqkleslges slrnftvdap
      661 tdsvyqfege dyrekqkilg lgnwieppkr erkanyavda yfrealrvse pkapkaprpp
      721 kqpivqdfqf fpprlfelld qeiyyfrqtv gykvpknpel gsdaariqke eqrkideaqp
      781 ltdeevaeke klltqgftnw tkrdfnqfik anekygrddi eniakevegk tpeevmeysa
      841 vfwerchelq didrvmaqie rgeakiqrra gikkaldakm aryrapfhql riaygtnkgk
      901 nyteeedrfl vcmlhklgfd kenvyeelra tvrsapqfrf dwfvksrtal elqrrcntli
      961 tlierenqel eererqerrk kggsigakpa skrkqenlpa pqdkprkkkk
//
LOCUS       XP_076548171             759 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylglucosamine-1-phosphate transferase subunits alpha and beta
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548171
VERSION     XP_076548171.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692056.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..759
                     /product="N-acetylglucosamine-1-phosphate transferase
                     subunits alpha and beta isoform X1"
                     /calculated_mol_wt=88416
     Region          102..129
                     /region_name="Stealth_CR1"
                     /note="Stealth protein CR1, conserved region 1; pfam17101"
                     /db_xref="CDD:465346"
     Region          136..243
                     /region_name="Stealth_CR2"
                     /note="Stealth protein CR2, conserved region 2; pfam11380"
                     /db_xref="CDD:463267"
     Region          256..283
                     /region_name="Notch"
                     /note="LNR domain; pfam00066"
                     /db_xref="CDD:459658"
     Region          469..517
                     /region_name="Stealth_CR3"
                     /note="Stealth protein CR3, conserved region 3; pfam17102"
                     /db_xref="CDD:465347"
     Region          650..707
                     /region_name="Stealth_CR4"
                     /note="Stealth protein CR4, conserved region 4; pfam17103"
                     /db_xref="CDD:465348"
     CDS             1..759
                     /gene="Gnptab"
                     /coded_by="XM_076692056.1:178..2457"
                     /db_xref="GeneID:143305194"
ORIGIN      
        1 mghvnsavyi ravilamlia ckvfinimst ckllqrrcyd llsykysllv iliaftcifi
       61 giihfgeiwi twskgkyeai fhsfndnilg isfqqklcqh vpidvvytwv ngsdpvflkn
      121 lkkhvpimdv ntapsrfndk delryslrsl emyapwvrhv yivtngqips wldmdnprlt
      181 lvthedifln ksdlptfssp aienhihrip gisdkflyfn ddvmlgaevw pedfitqvgg
      241 qkvylawwvp dcsdvcpwaw vgdgscdpac nttlcefdgg dcdsttvptd sealeeegdy
      301 phkflqdiqe ntnyglkfln ilgnrsihsg wndtetfthq tlaeakglkl nvnksysfrn
      361 nkamngiein fndhlnetis tqklllskvi nkikfpssni dkrihmkhyl ndndliltem
      421 nstartnnkp kysdhwkhkr qldtyaesll yvnriyniay gferrkvpah mphlidkwiv
      481 tdmqkkfehe fkktsshkvr nsedmqfafs yfyfltsekr ripieeifdk fdtdksgtws
      541 dreirtllsr lyplpldysl vvefentitn csnhlsitei snpppgeryl dsslpivske
      601 liancepisr kiearfgegk lypheiikag kneifemlts nvsltvqlld eirrdpkkfi
      661 clnddmdplr rseneivral lndfyrslyp lrstfelplq yrnlfahrre lfewranrak
      721 arnlllcliv lllvttlyhl fyhqirrlfr vralptllv
//
LOCUS       XP_076548172             632 aa            linear   INV 03-SEP-2025
DEFINITION  N-acetylglucosamine-1-phosphate transferase subunits alpha and beta
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548172
VERSION     XP_076548172.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692057.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..632
                     /product="N-acetylglucosamine-1-phosphate transferase
                     subunits alpha and beta isoform X2"
                     /calculated_mol_wt=73808
     Region          9..116
                     /region_name="Stealth_CR2"
                     /note="Stealth protein CR2, conserved region 2; pfam11380"
                     /db_xref="CDD:463267"
     Region          129..156
                     /region_name="Notch"
                     /note="LNR domain; pfam00066"
                     /db_xref="CDD:459658"
     Region          342..390
                     /region_name="Stealth_CR3"
                     /note="Stealth protein CR3, conserved region 3; pfam17102"
                     /db_xref="CDD:465347"
     Region          523..580
                     /region_name="Stealth_CR4"
                     /note="Stealth protein CR4, conserved region 4; pfam17103"
                     /db_xref="CDD:465348"
     CDS             1..632
                     /gene="Gnptab"
                     /coded_by="XM_076692057.1:554..2452"
                     /db_xref="GeneID:143305194"
ORIGIN      
        1 mdvntapsrf ndkdelrysl rslemyapwv rhvyivtngq ipswldmdnp rltlvthedi
       61 flnksdlptf sspaienhih ripgisdkfl yfnddvmlga evwpedfitq vggqkvylaw
      121 wvpdcsdvcp wawvgdgscd pacnttlcef dggdcdsttv ptdsealeee gdyphkflqd
      181 iqentnyglk flnilgnrsi hsgwndtetf thqtlaeakg lklnvnksys frnnkamngi
      241 einfndhlne tistqkllls kvinkikfps snidkrihmk hylndndlil temnstartn
      301 nkpkysdhwk hkrqldtyae sllyvnriyn iaygferrkv pahmphlidk wivtdmqkkf
      361 ehefkktssh kvrnsedmqf afsyfyflts ekrripieei fdkfdtdksg twsdreirtl
      421 lsrlyplpld yslvvefent itncsnhlsi teisnpppge ryldsslpiv skeliancep
      481 isrkiearfg egklypheii kagkneifem ltsnvsltvq lldeirrdpk kficlnddmd
      541 plrrseneiv rallndfyrs lyplrstfel plqyrnlfah rrelfewran rakarnlllc
      601 livlllvttl yhlfyhqirr lfrvralptl lv
//
LOCUS       XP_034191139             574 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 7 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191139
VERSION     XP_034191139.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335248.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..574
                     /product="tyrosine-protein phosphatase non-receptor type 7
                     isoform X1"
                     /calculated_mol_wt=63292
     Region          335..555
                     /region_name="PTPc-KIM"
                     /note="catalytic domain of the kinase interaction motif
                     (KIM) family of protein-tyrosine phosphatases; cd14547"
                     /db_xref="CDD:350395"
     CDS             1..574
                     /gene="LOC117609211"
                     /coded_by="XM_034335248.2:261..1985"
                     /db_xref="GeneID:117609211"
ORIGIN      
        1 mavytstqpl lsnhklqvnl nrtilvflan alltkggtst pltnhyvnda nyydissnnd
       61 nsdplekfyl iteqrwhtds vlpipllpst egmsarshhr ipqsitglnd agmdlqaaqf
      121 strtgqvvhs tatlswldwq lplfvalcai agailftflv llwlrkqmsr ekdtedgdrr
      181 glveegavcp pekttkatkg siewvhqpta kasihtvqsi kpltqatglp evmsvmtper
      241 rpepirikak rllerrgssa sltielappp espphivtpt rectaeefll sagnvlsrtq
      301 lkkaisdpss lhkefwevpl nlpdeldicg sgaknrycsv lpnpqsrvvl pgssddplss
      361 yinanyirgy dgedaryiat qgplshtvad fwkmvwadkv svvvmitklh eaaktkceay
      421 fpqdkngrvq agnftiians idtrdgytir dlelrceger rhvqhywyds wpdhavpqaa
      481 dtlvslaaev nslpgpvvvh csagigrtgc fialatgmtq llrdgnvdvl gilcqmrydr
      541 ggmvqtaeqy efvhralcly eqtldggkss ssge
//
LOCUS       XP_034191140             562 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 7 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191140
VERSION     XP_034191140.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335249.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..562
                     /product="tyrosine-protein phosphatase non-receptor type 7
                     isoform X2"
                     /calculated_mol_wt=62177
     Region          323..543
                     /region_name="PTPc-KIM"
                     /note="catalytic domain of the kinase interaction motif
                     (KIM) family of protein-tyrosine phosphatases; cd14547"
                     /db_xref="CDD:350395"
     CDS             1..562
                     /gene="LOC117609211"
                     /coded_by="XM_034335249.2:246..1934"
                     /db_xref="GeneID:117609211"
ORIGIN      
        1 mekyarfdtc vnmrqcfdgi fvhggtstpl tnhyvndany ydissnndns dplekfylit
       61 eqrwhtdsvl pipllpsteg msarshhrip qsitglndag mdlqaaqfst rtgqvvhsta
      121 tlswldwqlp lfvalcaiag ailftflvll wlrkqmsrek dtedgdrrgl veegavcppe
      181 kttkatkgsi ewvhqptaka sihtvqsikp ltqatglpev msvmtperrp epirikakrl
      241 lerrgssasl tielapppes pphivtptre ctaeefllsa gnvlsrtqlk kaisdpsslh
      301 kefwevplnl pdeldicgsg aknrycsvlp npqsrvvlpg ssddplssyi nanyirgydg
      361 edaryiatqg plshtvadfw kmvwadkvsv vvmitklhea aktkceayfp qdkngrvqag
      421 nftiiansid trdgytirdl elrcegerrh vqhywydswp dhavpqaadt lvslaaevns
      481 lpgpvvvhcs agigrtgcfi alatgmtqll rdgnvdvlgi lcqmrydrgg mvqtaeqyef
      541 vhralclyeq tldggkssss ge
//
LOCUS       XP_034191141             542 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 7 isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191141
VERSION     XP_034191141.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335250.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..542
                     /product="tyrosine-protein phosphatase non-receptor type 7
                     isoform X3"
                     /calculated_mol_wt=59799
     Region          303..523
                     /region_name="PTPc-KIM"
                     /note="catalytic domain of the kinase interaction motif
                     (KIM) family of protein-tyrosine phosphatases; cd14547"
                     /db_xref="CDD:350395"
     CDS             1..542
                     /gene="LOC117609211"
                     /coded_by="XM_034335250.2:16..1644"
                     /db_xref="GeneID:117609211"
ORIGIN      
        1 mgkrgtstpl tnhyvndany ydissnndns dplekfylit eqrwhtdsvl pipllpsteg
       61 msarshhrip qsitglndag mdlqaaqfst rtgqvvhsta tlswldwqlp lfvalcaiag
      121 ailftflvll wlrkqmsrek dtedgdrrgl veegavcppe kttkatkgsi ewvhqptaka
      181 sihtvqsikp ltqatglpev msvmtperrp epirikakrl lerrgssasl tielapppes
      241 pphivtptre ctaeefllsa gnvlsrtqlk kaisdpsslh kefwevplnl pdeldicgsg
      301 aknrycsvlp npqsrvvlpg ssddplssyi nanyirgydg edaryiatqg plshtvadfw
      361 kmvwadkvsv vvmitklhea aktkceayfp qdkngrvqag nftiiansid trdgytirdl
      421 elrcegerrh vqhywydswp dhavpqaadt lvslaaevns lpgpvvvhcs agigrtgcfi
      481 alatgmtqll rdgnvdvlgi lcqmrydrgg mvqtaeqyef vhralclyeq tldggkssss
      541 ge
//
LOCUS       XP_034191142             482 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 7 isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191142
VERSION     XP_034191142.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335251.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..482
                     /product="tyrosine-protein phosphatase non-receptor type 7
                     isoform X4"
                     /calculated_mol_wt=52990
     Region          243..463
                     /region_name="PTPc-KIM"
                     /note="catalytic domain of the kinase interaction motif
                     (KIM) family of protein-tyrosine phosphatases; cd14547"
                     /db_xref="CDD:350395"
     CDS             1..482
                     /gene="LOC117609211"
                     /coded_by="XM_034335251.2:758..2206"
                     /db_xref="GeneID:117609211"
ORIGIN      
        1 msarshhrip qsitglndag mdlqaaqfst rtgqvvhsta tlswldwqlp lfvalcaiag
       61 ailftflvll wlrkqmsrek dtedgdrrgl veegavcppe kttkatkgsi ewvhqptaka
      121 sihtvqsikp ltqatglpev msvmtperrp epirikakrl lerrgssasl tielapppes
      181 pphivtptre ctaeefllsa gnvlsrtqlk kaisdpsslh kefwevplnl pdeldicgsg
      241 aknrycsvlp npqsrvvlpg ssddplssyi nanyirgydg edaryiatqg plshtvadfw
      301 kmvwadkvsv vvmitklhea aktkceayfp qdkngrvqag nftiiansid trdgytirdl
      361 elrcegerrh vqhywydswp dhavpqaadt lvslaaevns lpgpvvvhcs agigrtgcfi
      421 alatgmtqll rdgnvdvlgi lcqmrydrgg mvqtaeqyef vhralclyeq tldggkssss
      481 ge
//
LOCUS       XP_034191143             482 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 7 isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191143
VERSION     XP_034191143.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335252.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..482
                     /product="tyrosine-protein phosphatase non-receptor type 7
                     isoform X4"
                     /calculated_mol_wt=52990
     Region          243..463
                     /region_name="PTPc-KIM"
                     /note="catalytic domain of the kinase interaction motif
                     (KIM) family of protein-tyrosine phosphatases; cd14547"
                     /db_xref="CDD:350395"
     CDS             1..482
                     /gene="LOC117609211"
                     /coded_by="XM_034335252.2:325..1773"
                     /db_xref="GeneID:117609211"
ORIGIN      
        1 msarshhrip qsitglndag mdlqaaqfst rtgqvvhsta tlswldwqlp lfvalcaiag
       61 ailftflvll wlrkqmsrek dtedgdrrgl veegavcppe kttkatkgsi ewvhqptaka
      121 sihtvqsikp ltqatglpev msvmtperrp epirikakrl lerrgssasl tielapppes
      181 pphivtptre ctaeefllsa gnvlsrtqlk kaisdpsslh kefwevplnl pdeldicgsg
      241 aknrycsvlp npqsrvvlpg ssddplssyi nanyirgydg edaryiatqg plshtvadfw
      301 kmvwadkvsv vvmitklhea aktkceayfp qdkngrvqag nftiiansid trdgytirdl
      361 elrcegerrh vqhywydswp dhavpqaadt lvslaaevns lpgpvvvhcs agigrtgcfi
      421 alatgmtqll rdgnvdvlgi lcqmrydrgg mvqtaeqyef vhralclyeq tldggkssss
      481 ge
//
LOCUS       XP_034191137             500 aa            linear   INV 03-SEP-2025
DEFINITION  putative fatty acyl-CoA reductase CG5065 [Osmia lignaria lignaria].
ACCESSION   XP_034191137
VERSION     XP_034191137.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335246.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..500
                     /product="putative fatty acyl-CoA reductase CG5065"
                     /calculated_mol_wt=56647
     Region          28..342
                     /region_name="FAR-N_SDR_e"
                     /note="fatty acyl CoA reductases (FARs), extended (e)
                     SDRs; cd05236"
                     /db_xref="CDD:187547"
     Site            order(32,34..37,59..61,124..126,143,164..166,217,221,
                     242..245)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Site            order(144,166,217,221)
                     /site_type="active"
                     /db_xref="CDD:187547"
     Site            order(166,217,244,256,272,278)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Region          368..458
                     /region_name="Sterile"
                     /note="Male sterility protein; pfam03015"
                     /db_xref="CDD:460779"
     CDS             1..500
                     /gene="LOC117609210"
                     /coded_by="XM_034335246.2:302..1804"
                     /db_xref="GeneID:117609210"
ORIGIN      
        1 mgttnaqtnt ekgsdetsid affaesvili tgatgfvgka lvekllrvcp rlaiifilir
       61 pkrnqtveqr ftsflesfvf deirssklls kikpvkgdva lpdlglsqed ksmltervni
      121 ifhsaatvkf nepvkvavnl ntngtdrmie lsksmknlis fihistaysn snrvdieevi
      181 yptnvkpsti idmcesldnd alnvlekkvl eihpntytft kklaeqiile kgkdlpiaiv
      241 rpsiigaaek epfpgwvdni fgatatliei gngnvksvvt nsklkcdlip vdrvidtlic
      301 aawytsvqkr dtikvynctn sasplswgtl igltkkyaie tpsqyvmwyp cctcrqnrfi
      361 hnlilftlni fpaflidifv kltggkpimm klanrvlrla easeyftmhe whfhrdnlye
      421 lakkvkslkd snnfvvised lnwesylrsy ilgirtfiqk dtpetlktar nrlhvlywlr
      481 rftqvfsift vlmiilriah
//
LOCUS       XP_034191138             500 aa            linear   INV 03-SEP-2025
DEFINITION  putative fatty acyl-CoA reductase CG5065 [Osmia lignaria lignaria].
ACCESSION   XP_034191138
VERSION     XP_034191138.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335247.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..500
                     /product="putative fatty acyl-CoA reductase CG5065"
                     /calculated_mol_wt=56647
     Region          28..342
                     /region_name="FAR-N_SDR_e"
                     /note="fatty acyl CoA reductases (FARs), extended (e)
                     SDRs; cd05236"
                     /db_xref="CDD:187547"
     Site            order(32,34..37,59..61,124..126,143,164..166,217,221,
                     242..245)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Site            order(144,166,217,221)
                     /site_type="active"
                     /db_xref="CDD:187547"
     Site            order(166,217,244,256,272,278)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187547"
     Region          368..458
                     /region_name="Sterile"
                     /note="Male sterility protein; pfam03015"
                     /db_xref="CDD:460779"
     CDS             1..500
                     /gene="LOC117609210"
                     /coded_by="XM_034335247.2:308..1810"
                     /db_xref="GeneID:117609210"
ORIGIN      
        1 mgttnaqtnt ekgsdetsid affaesvili tgatgfvgka lvekllrvcp rlaiifilir
       61 pkrnqtveqr ftsflesfvf deirssklls kikpvkgdva lpdlglsqed ksmltervni
      121 ifhsaatvkf nepvkvavnl ntngtdrmie lsksmknlis fihistaysn snrvdieevi
      181 yptnvkpsti idmcesldnd alnvlekkvl eihpntytft kklaeqiile kgkdlpiaiv
      241 rpsiigaaek epfpgwvdni fgatatliei gngnvksvvt nsklkcdlip vdrvidtlic
      301 aawytsvqkr dtikvynctn sasplswgtl igltkkyaie tpsqyvmwyp cctcrqnrfi
      361 hnlilftlni fpaflidifv kltggkpimm klanrvlrla easeyftmhe whfhrdnlye
      421 lakkvkslkd snnfvvised lnwesylrsy ilgirtfiqk dtpetlktar nrlhvlywlr
      481 rftqvfsift vlmiilriah
//
LOCUS       XP_034191145             412 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609212 [Osmia lignaria lignaria].
ACCESSION   XP_034191145
VERSION     XP_034191145.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335254.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191145.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..412
                     /product="uncharacterized protein LOC117609212"
                     /calculated_mol_wt=45975
     Region          10..52
                     /region_name="DD_ROP"
                     /note="dimerization/docking (D/D) domain found in
                     ropporins; cd23019"
                     /db_xref="CDD:438555"
     Site            order(13..14,16,18..19,22..23,26..27,30..31,34,38..40,
                     42..43,46..47,49..50)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:438555"
     Site            order(15,19..20,23..24,27..28,31..32)
                     /site_type="other"
                     /note="putative AKAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:438555"
     CDS             1..412
                     /gene="LOC117609212"
                     /coded_by="XM_034335254.2:272..1510"
                     /db_xref="GeneID:117609212"
ORIGIN      
        1 msiveqsvvg qeivvppslp vilkqfckaa irtqpydllk wsssyfrala dgeepptklr
       61 leypppctas gltfgflrvl lrqfgdynks lpvetilrrw dclcldrrdl nlimmigkfr
      121 rkcqvkkfla iavgllgssl fetmimicel fthepeggsa mvpvtlfmei ygylaglrcd
      181 gserdssded ptefiirdnv ssasqeskss eeksvdrvcs vasqdteadv nfdlelvsrd
      241 dvdssrtdtm pqlidcytge kneenktser dtsnkealkv kksnetfate aesskksess
      301 egkelnkden rsaksasscs tteenttknq sarstgdksa nerrrkkkiv dsklvkyypn
      361 ipgigprlsa eevasvaiwm secarvqegm vgprnlrhmn cppldrqeis es
//
LOCUS       XP_034191146             363 aa            linear   INV 03-SEP-2025
DEFINITION  septate junction protein lachesin [Osmia lignaria lignaria].
ACCESSION   XP_034191146
VERSION     XP_034191146.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335255.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..363
                     /product="septate junction protein lachesin"
                     /calculated_mol_wt=40462
     Region          29..128
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          39..43
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409381"
     Region          52..56
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409381"
     Region          93..97
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409381"
     Region          107..112
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409381"
     Region          121..124
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409381"
     Region          138..220
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          150..154
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409394"
     Region          163..167
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409394"
     Region          184..188
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409394"
     Region          198..203
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409394"
     Region          212..215
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409394"
     Region          223..315
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          253..257
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          283..287
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          297..302
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     CDS             1..363
                     /gene="Lac"
                     /coded_by="XM_034335255.2:57..1148"
                     /db_xref="GeneID:117609214"
ORIGIN      
        1 mkimyiilvf vflqavsgqr tptishitqe qikdigesve frcsvqyaqd fpviwikink
       61 dsandqlpls hgssliitdt rfalkhdeat stfilqvkdi qetdagfyqc kvlislnnyv
      121 tanvelqvrr ppvisdnstr slvvsegqpv qldcyaggfp spriswrren nailptggsi
      181 yrgntlkisa irkedrgtyy cvaengvgrg arrninveve fapvitaprp rlgqalqydm
      241 dlechveayp ppaiiwlkdd vqlsnnqhys ishfatadqy tdttirvitv ekrqygeyvc
      301 raanklgtae ttvelfetsv pvcppacgqa iyygagverv ssgllvvlvl fitvslrnfh
      361 sqy
//
LOCUS       XP_034191147             339 aa            linear   INV 03-SEP-2025
DEFINITION  sorbitol dehydrogenase [Osmia lignaria lignaria].
ACCESSION   XP_034191147
VERSION     XP_034191147.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335256.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..339
                     /product="sorbitol dehydrogenase"
                     /calculated_mol_wt=36541
     Region          10..337
                     /region_name="MDR"
                     /note="Medium chain reductase/dehydrogenase
                     (MDR)/zinc-dependent alcohol dehydrogenase-like family;
                     cl16912"
                     /db_xref="CDD:450120"
     Site            order(38..40,43,149,153,174..179,199..200,204,219,
                     233..234,256..257,282..284)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176196"
     CDS             1..339
                     /gene="LOC117609215"
                     /coded_by="XM_034335256.2:62..1081"
                     /db_xref="GeneID:117609215"
ORIGIN      
        1 meflsfdvtk ktlalrraev pvpapnevrv rvaysgicgt dlhildgtfp ckkeglltlg
       61 hefsgtidav gsavrnlkvg qkvvvdpnsg cnlcdycrvg dyhfcenggi nntvgifrdg
      121 gwsthavvpe gqvyllpegv gmaqaaltep lsclahgwdk inpvnvglnv lvlgagiigl
      181 lwscllhlhg lrktvtvsep qekrrnavkk lgldygvksp telksgfdlv vdcsgsgpam
      241 eaavpllnrg grlcvfgvas pkakltiepf qiymkelkiv gvninpftfh kgiallqama
      301 dtylnydklc iktyslseyp kalealkrge iskavfklq
//
LOCUS       XP_034191148             287 aa            linear   INV 03-SEP-2025
DEFINITION  CUE domain-containing protein 2-A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191148
VERSION     XP_034191148.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335257.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="CUE domain-containing protein 2-A isoform X1"
                     /calculated_mol_wt=32089
     Region          147..187
                     /region_name="CUE_CUED2"
                     /note="CUE domain found in CUE domain-containing protein 2
                     (CUED2) and similar proteins; cd14367"
                     /db_xref="CDD:270550"
     CDS             1..287
                     /gene="LOC117609216"
                     /coded_by="XM_034335257.2:150..1013"
                     /db_xref="GeneID:117609216"
ORIGIN      
        1 mnrtmdekee lvkkslfsfv rkevptaqls liddivlsyv vsmveesale edldvdglce
       61 mvsaclpefs tiekeavskw lldiesklrq eskenencqp hdplshislt allppgtqrm
      121 rvhhlsetsd agsdssgeyf seqesswhqv allqemfpaa slaearhcla vasgdiakaa
      181 qlalhrqeag qsivsnltfl tpngrnkarv ndeelksrii arysyvdrdd dsrehrpvap
      241 ktepkklvry ldnkivsvkg erytevrrgg eeedgneggr kkghsrp
//
LOCUS       XP_034191150             287 aa            linear   INV 03-SEP-2025
DEFINITION  CUE domain-containing protein 2-A isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191150
VERSION     XP_034191150.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335259.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..287
                     /product="CUE domain-containing protein 2-A isoform X1"
                     /calculated_mol_wt=32089
     Region          147..187
                     /region_name="CUE_CUED2"
                     /note="CUE domain found in CUE domain-containing protein 2
                     (CUED2) and similar proteins; cd14367"
                     /db_xref="CDD:270550"
     CDS             1..287
                     /gene="LOC117609216"
                     /coded_by="XM_034335259.2:238..1101"
                     /db_xref="GeneID:117609216"
ORIGIN      
        1 mnrtmdekee lvkkslfsfv rkevptaqls liddivlsyv vsmveesale edldvdglce
       61 mvsaclpefs tiekeavskw lldiesklrq eskenencqp hdplshislt allppgtqrm
      121 rvhhlsetsd agsdssgeyf seqesswhqv allqemfpaa slaearhcla vasgdiakaa
      181 qlalhrqeag qsivsnltfl tpngrnkarv ndeelksrii arysyvdrdd dsrehrpvap
      241 ktepkklvry ldnkivsvkg erytevrrgg eeedgneggr kkghsrp
//
LOCUS       XP_034191151             286 aa            linear   INV 03-SEP-2025
DEFINITION  CUE domain-containing protein 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191151
VERSION     XP_034191151.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335260.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..286
                     /product="CUE domain-containing protein 2 isoform X2"
                     /calculated_mol_wt=31961
     Region          146..186
                     /region_name="CUE_CUED2"
                     /note="CUE domain found in CUE domain-containing protein 2
                     (CUED2) and similar proteins; cd14367"
                     /db_xref="CDD:270550"
     CDS             1..286
                     /gene="LOC117609216"
                     /coded_by="XM_034335260.2:150..1010"
                     /db_xref="GeneID:117609216"
ORIGIN      
        1 mnrtmdekee lvkkslfsfv rkevptaqls liddivlsyv vsmveesale edldvdglce
       61 mvsaclpefs tiekeavskw lldiesklrq eskenencqp hdplshislt allppgtqrm
      121 rvhhlsetsd agsdssgeyf seesswhqva llqemfpaas laearhclav asgdiakaaq
      181 lalhrqeagq sivsnltflt pngrnkarvn deelksriia rysyvdrddd srehrpvapk
      241 tepkklvryl dnkivsvkge rytevrrgge eedgneggrk kghsrp
//
LOCUS       XP_034191152             154 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609217 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191152
VERSION     XP_034191152.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335261.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..154
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..154
                     /product="uncharacterized protein LOC117609217 isoform X1"
                     /calculated_mol_wt=17308
     CDS             1..154
                     /gene="LOC117609217"
                     /coded_by="XM_034335261.2:160..624"
                     /db_xref="GeneID:117609217"
ORIGIN      
        1 myttenknts grswiviami lavfhlpaiv saqlgferfk sentiatydn vlnfakvgen
       61 dvakqlneel lgtaktrhkr lsdqrraele tlmalskmte klvnvirggr hldltksgrr
      121 krsifdlnvk nlqklfrlsg kigykgnkay pvvs
//
LOCUS       XP_034191153             124 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609217 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191153
VERSION     XP_034191153.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335262.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..124
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..124
                     /product="uncharacterized protein LOC117609217 isoform X2"
                     /calculated_mol_wt=13900
     CDS             1..124
                     /gene="LOC117609217"
                     /coded_by="XM_034335262.2:160..534"
                     /db_xref="GeneID:117609217"
ORIGIN      
        1 myttenknts grswiviami lavfhlpaiv saqlgferfk sentiatydn vlnfakvgen
       61 dvakqlneel lgtaktrhkr lsdqrraele tlmalskmte klvnvirggr hldltksenr
      121 lqgk
//
LOCUS       XP_034191154             134 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609218 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191154
VERSION     XP_034191154.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335263.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..134
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..134
                     /product="uncharacterized protein LOC117609218 isoform X1"
                     /calculated_mol_wt=15021
     CDS             1..134
                     /gene="LOC117609218"
                     /coded_by="XM_034335263.2:253..657"
                     /db_xref="GeneID:117609218"
ORIGIN      
        1 mqsingvksl fvtlmvlsvl dlilmpttth arpppsllsr qrrvsdqrla eietiiglen
       61 vkgkvvtvpv gfgvvdinki grrrrsatnn rletlqrilr ivandpelld dekilslpmk
      121 mkesrhaand ewfi
//
LOCUS       XP_034191155             133 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609218 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191155
VERSION     XP_034191155.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335264.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..133
                     /product="uncharacterized protein LOC117609218 isoform X2"
                     /calculated_mol_wt=14934
     CDS             1..133
                     /gene="LOC117609218"
                     /coded_by="XM_034335264.2:253..654"
                     /db_xref="GeneID:117609218"
ORIGIN      
        1 mqsingvksl fvtlmvlsvl dlilmpttth arpppsllrq rrvsdqrlae ietiiglenv
       61 kgkvvtvpvg fgvvdinkig rrrrsatnnr letlqrilri vandpelldd ekilslpmkm
      121 kesrhaande wfi
//
LOCUS       XP_034187210             490 aa            linear   INV 03-SEP-2025
DEFINITION  spaetzle domain-containing protein 4 [Osmia lignaria lignaria].
ACCESSION   XP_034187210
VERSION     XP_034187210.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331319.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..490
                     /product="spaetzle domain-containing protein 4"
                     /calculated_mol_wt=56140
     Region          369..457
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..490
                     /gene="spz4"
                     /coded_by="XM_034331319.2:208..1680"
                     /db_xref="GeneID:117607528"
ORIGIN      
        1 mnvivhlvlq vityqlvqcq gsggpstfgy dassacskpy srsgrarlan lpcdfrrqnw
       61 ctvagtsypw havrrfvhen qglmrrmygd erhinvlkae lekndielry deyyhnfade
      121 hrkyqyshdy eyfegdseye grgftsrafn dnsakrlnkf pklseytrph frptekstts
      181 taatpapsts ttssttettt patsitrest tkptssttit tnstnddtre ntteeaialt
      241 vttshmmkeq nfsineipeq idrideelde ddevaevsei sldetstass pgitelpsdp
      301 dvsedllvtp kettvqeeis gsneqefrpr peyrpagkpe tsnmegqlyq dvaakdqqeq
      361 pvlklrgvna cpvkeevvap fwanntrgev lallnlypfe qyvhwekcth enkqmycrdg
      421 crceqqyrlh rllaydpnne crgifsdwfk fpsccvcrcy dlplefrvts rsprsqkqri
      481 kvrsprsryt
//
LOCUS       XP_034183045             966 aa            linear   INV 03-SEP-2025
DEFINITION  stork-head domain-containing protein knockout isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183045
VERSION     XP_034183045.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327154.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..966
                     /product="stork-head domain-containing protein knockout
                     isoform X1"
                     /calculated_mol_wt=105378
     Region          140..218
                     /region_name="Stork_head"
                     /note="Winged helix Storkhead-box1 domain; pfam10264"
                     /db_xref="CDD:431182"
     CDS             1..966
                     /gene="knockout"
                     /coded_by="XM_034327154.2:867..3767"
                     /db_xref="GeneID:117605625"
ORIGIN      
        1 masilgdgtg pggsgpsrcl lllqrslaiq ltrgpppqil nasqrespke hpademwmyd
       61 kgynlfqsfl eanskcwwna alvdatrqlr ykghvapgvl mvggppcale vlraawarnv
      121 lrppadhait clgdiedclv apvaqgqftp lpealcwail eltsqrqaat ldtlrsalrn
      181 afpamqrpsr elvydalakl mqerkiyhts qgyfvvtpet rrlrrdssss rrcsrevsgs
      241 rsqgsmlmsn deamalvhge mltlrdgdvt hqavqtnlad vicggnpndk vlfarcrsmp
      301 grlerrhslr lwgsarrlhr sgssrslprr persdtsssa eyastdsaph sptsfsgifs
      361 ekigllsrlf rrstrrkgap lmgtfsaqyp ptewfnprvv hlhsvatqtr aaspsmmegl
      421 dqsqasfqvw ddsssvrsat lprrhrrqas gdsssllans tprssrrhrs pcstlprskc
      481 sslsrctsra svlppstnqe syqsrnqtqn gknstnssps rsggssgsvr tanaspakta
      541 tlgrsstrqs nsrrpshdst vsgmktngsp qhkvlqktss ghsshssgks isssklsssp
      601 lktstlpaks splkvvqqgs ltplqeaqns itlqvstnlt pvspskeqra iqnsvtlasn
      661 atstttisgs pgtkiyvqqn nspmrsvitf engtvktkla dketldckek qdreligeki
      721 fkskideekl fksklddekl nksskierps slyaskeskp shllsesdke nkpkveeevp
      781 nklkltnrln nsqahlidgs tnnidknslv nehptsalss mkntndamnn srklslslsk
      841 dalsyrnllr pdsknniasn ppsptksfdg fggsfnnvyi enlesakqqr apqgsepnle
      901 ktvsrgdlys ypslsdmqvq ftslaaqkil kgcpinsvdt lvevnmaaae kpnncdvtvh
      961 tdfglv
//
LOCUS       XP_034183046             966 aa            linear   INV 03-SEP-2025
DEFINITION  stork-head domain-containing protein knockout isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183046
VERSION     XP_034183046.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327155.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..966
                     /product="stork-head domain-containing protein knockout
                     isoform X1"
                     /calculated_mol_wt=105378
     Region          140..218
                     /region_name="Stork_head"
                     /note="Winged helix Storkhead-box1 domain; pfam10264"
                     /db_xref="CDD:431182"
     CDS             1..966
                     /gene="knockout"
                     /coded_by="XM_034327155.2:690..3590"
                     /db_xref="GeneID:117605625"
ORIGIN      
        1 masilgdgtg pggsgpsrcl lllqrslaiq ltrgpppqil nasqrespke hpademwmyd
       61 kgynlfqsfl eanskcwwna alvdatrqlr ykghvapgvl mvggppcale vlraawarnv
      121 lrppadhait clgdiedclv apvaqgqftp lpealcwail eltsqrqaat ldtlrsalrn
      181 afpamqrpsr elvydalakl mqerkiyhts qgyfvvtpet rrlrrdssss rrcsrevsgs
      241 rsqgsmlmsn deamalvhge mltlrdgdvt hqavqtnlad vicggnpndk vlfarcrsmp
      301 grlerrhslr lwgsarrlhr sgssrslprr persdtsssa eyastdsaph sptsfsgifs
      361 ekigllsrlf rrstrrkgap lmgtfsaqyp ptewfnprvv hlhsvatqtr aaspsmmegl
      421 dqsqasfqvw ddsssvrsat lprrhrrqas gdsssllans tprssrrhrs pcstlprskc
      481 sslsrctsra svlppstnqe syqsrnqtqn gknstnssps rsggssgsvr tanaspakta
      541 tlgrsstrqs nsrrpshdst vsgmktngsp qhkvlqktss ghsshssgks isssklsssp
      601 lktstlpaks splkvvqqgs ltplqeaqns itlqvstnlt pvspskeqra iqnsvtlasn
      661 atstttisgs pgtkiyvqqn nspmrsvitf engtvktkla dketldckek qdreligeki
      721 fkskideekl fksklddekl nksskierps slyaskeskp shllsesdke nkpkveeevp
      781 nklkltnrln nsqahlidgs tnnidknslv nehptsalss mkntndamnn srklslslsk
      841 dalsyrnllr pdsknniasn ppsptksfdg fggsfnnvyi enlesakqqr apqgsepnle
      901 ktvsrgdlys ypslsdmqvq ftslaaqkil kgcpinsvdt lvevnmaaae kpnncdvtvh
      961 tdfglv
//
LOCUS       XP_034183047             959 aa            linear   INV 03-SEP-2025
DEFINITION  stork-head domain-containing protein knockout isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034183047
VERSION     XP_034183047.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327156.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..959
                     /product="stork-head domain-containing protein knockout
                     isoform X2"
                     /calculated_mol_wt=104651
     Region          140..218
                     /region_name="Stork_head"
                     /note="Winged helix Storkhead-box1 domain; pfam10264"
                     /db_xref="CDD:431182"
     CDS             1..959
                     /gene="knockout"
                     /coded_by="XM_034327156.2:867..3746"
                     /db_xref="GeneID:117605625"
ORIGIN      
        1 masilgdgtg pggsgpsrcl lllqrslaiq ltrgpppqil nasqrespke hpademwmyd
       61 kgynlfqsfl eanskcwwna alvdatrqlr ykghvapgvl mvggppcale vlraawarnv
      121 lrppadhait clgdiedclv apvaqgqftp lpealcwail eltsqrqaat ldtlrsalrn
      181 afpamqrpsr elvydalakl mqerkiyhts qgyfvvtpet rrlrrdssss rrcsrevsgs
      241 rsqgsmlmsn deamalvhge mltlrdgdvt hqavqtnlad vicggnpndk vlfarcrsmp
      301 grlerrhslr lwgsarrlhr sgssrslprr persdtsssa eyastdsaph sptkkiglls
      361 rlfrrstrrk gaplmgtfsa qypptewfnp rvvhlhsvat qtraaspsmm egldqsqasf
      421 qvwddsssvr satlprrhrr qasgdsssll anstprssrr hrspcstlpr skcsslsrct
      481 srasvlppst nqesyqsrnq tqngknstns spsrsggssg svrtanaspa ktatlgrsst
      541 rqsnsrrpsh dstvsgmktn gspqhkvlqk tssghsshss gksissskls ssplktstlp
      601 akssplkvvq qgsltplqea qnsitlqvst nltpvspske qraiqnsvtl asnatsttti
      661 sgspgtkiyv qqnnspmrsv itfengtvkt kladketldc kekqdrelig ekifkskide
      721 eklfkskldd eklnksskie rpsslyaske skpshllses dkenkpkvee evpnklkltn
      781 rlnnsqahli dgstnnidkn slvnehptsa lssmkntnda mnnsrklsls lskdalsyrn
      841 llrpdsknni asnppsptks fdgfggsfnn vyienlesak qqrapqgsep nlektvsrgd
      901 lysypslsdm qvqftslaaq kilkgcpins vdtlvevnma aaekpnncdv tvhtdfglv
//
LOCUS       XP_034182833             614 aa            linear   INV 03-SEP-2025
DEFINITION  leucine-rich repeat-containing protein 40 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182833
VERSION     XP_034182833.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326942.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..614
                     /product="leucine-rich repeat-containing protein 40"
                     /calculated_mol_wt=69471
     Region          2..339
                     /region_name="LRR"
                     /note="Leucine-rich repeat (LRR) protein [Transcription];
                     COG4886"
                     /db_xref="CDD:443914"
     Region          96..118
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          119..141
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          127..>587
                     /region_name="PLN00113"
                     /note="leucine-rich repeat receptor-like protein kinase;
                     Provisional"
                     /db_xref="CDD:215061"
     Region          142..164
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          165..187
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          188..210
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          211..233
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          234..255
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          256..277
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          278..301
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          302..323
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          348..420
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          421..462
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          463..483
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          507..529
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          530..553
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     Region          554..576
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275380"
     CDS             1..614
                     /gene="LOC117605523"
                     /coded_by="XM_034326942.2:23..1867"
                     /db_xref="GeneID:117605523"
ORIGIN      
        1 mnyvstknnm sdakkkvdhl aifkqrtktd dnaemsevti isarktgnln lssrglftvp
       61 drvwsinelt dqevrdlhye ldyvheserw weqeplktld lsfnslaiid skieclselt
      121 tlllhdnlle dlpseignln klkilnlsnn klkqlpsqfy klnelqelcl knnsinildp
      181 avgdlimlth ldlsynnlse lpvgmgylvr lksldlshnm lielppdltn mralkkldas
      241 cnqlevlppl gelrkvetmm lqtnklitfp disgctllrv lhladnnite idmsclegvg
      301 rlkvltlgnn kietipeeim klvyleifdl shnkltivps qvgimpnlkq fvidgndvqn
      361 iradiircgt prilkhirqs ikstsnlstk ecvitddnps iypdkytmqs tkllslagqn
      421 lielphevle nackadvstv dlsrnklsel pdqlcmiati adlklasnel thipewigek
      481 ykylqaldls knfleslpst lgllkylrei nisfnrykei pesvydidsl eiliandnsi
      541 tnidvsylqk lqklatlnlg nnnigyvppe lgnlknirnl ilsgncfkqp rqailakste
      601 eilaylrdri prsn
//
LOCUS       XP_034182834             518 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605524 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182834
VERSION     XP_034182834.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326943.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..518
                     /product="uncharacterized protein LOC117605524 isoform X1"
                     /calculated_mol_wt=53333
     Region          401..457
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..518
                     /gene="LOC117605524"
                     /coded_by="XM_034326943.2:5385..6941"
                     /db_xref="GeneID:117605524"
ORIGIN      
        1 mgtatgagns tgvtwwammn gslyedsppp vpptasslvs iqqqqqqqat stpgshqqat
       61 tptspaapas atttvppapt asasstspsa ssvasnsgpg plhipakrlt atpagssyge
      121 vgcvdssaap aagpagvirh shsssagsqg gwsysphhpq eshyssaaaa dslnhhqtyg
      181 gnnpptyynl aadptstsgs srdnrkagtl sfwspaaata gsaatpgaas dyksynsvgs
      241 attssstgag sssvpsgvtd pavsschqsf sqswcnyapy ttarhhhpvd taghhhphsq
      301 agvpyltpsa addrgrvtaa mvaaegafph dgygglrnyg apepvtsspy pppvmwgssp
      361 sslfpagsla gvgvgvgvag mgvgcgssna lewaagqatv rkkrkpyskf qtlelekefl
      421 fnayvskqkr welarnlnlt erqvkiwfqn rrmknkknnq rqtqqqnnnn nnnssannan
      481 hhtatgshhh qsaahappss hhvvaqahha ngsakhhq
//
LOCUS       XP_076548174             505 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605524 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548174
VERSION     XP_076548174.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692059.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="uncharacterized protein LOC117605524 isoform X2"
                     /calculated_mol_wt=51985
     Region          388..444
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..505
                     /gene="LOC117605524"
                     /coded_by="XM_076692059.1:5386..6903"
                     /db_xref="GeneID:117605524"
ORIGIN      
        1 mgtatgagns tgvtwwammn gslyedsppp vpptasslvs iqqqqqqqat stpgshqqat
       61 tptspaapas atttvppapt asasstspsa ssvasnsgpg plhipakrlt atpagssyge
      121 vgcvdssaap aagpagvirh shsssagsqg gwsysphhpq eshyssaaaa dslnhhqtyg
      181 gnnpptyynl aadptstsgs srdnrkagtl sfwspaaata gsaatpgaas dyksynsvgs
      241 attssstgag sssvpsgvtd pavsschqsf sqswcnyapy ttarhhhpvd taghhhphsq
      301 agvpyltpsa addrgrvtaa mvaaegafph dgygglrnyg apepvtsspy pppgslagvg
      361 vgvgvagmgv gcgssnalew aagqatvrkk rkpyskfqtl elekeflfna yvskqkrwel
      421 arnlnlterq vkiwfqnrrm knkknnqrqt qqqnnnnnnn ssannanhht atgshhhqsa
      481 ahappsshhv vaqahhangs akhhq
//
LOCUS       XP_034182835             501 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605524 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034182835
VERSION     XP_034182835.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326944.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="uncharacterized protein LOC117605524 isoform X3"
                     /calculated_mol_wt=51683
     Region          384..440
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..501
                     /gene="LOC117605524"
                     /coded_by="XM_034326944.2:173..1678"
                     /db_xref="GeneID:117605524"
ORIGIN      
        1 mmngslyeds pppvpptass lvsiqqqqqq qatstpgshq qattptspaa pasatttvpp
       61 aptasassts psassvasns gpgplhipak rltatpagss ygevgcvdss aapaagpagv
      121 irhshsssag sqggwsysph hpqeshyssa aaadslnhhq tyggnnppty ynlaadptst
      181 sgssrdnrka gtlsfwspaa atagsaatpg aasdyksyns vgsattssst gagsssvpsg
      241 vtdpavssch qsfsqswcny apyttarhhh pvdtaghhhp hsqagvpylt psaaddrgrv
      301 taamvaaega fphdgygglr nygapepvts spypppvmwg sspsslfpag slagvgvgvg
      361 vagmgvgcgs snalewaagq atvrkkrkpy skfqtlelek eflfnayvsk qkrwelarnl
      421 nlterqvkiw fqnrrmknkk nnqrqtqqqn nnnnnnssan nanhhtatgs hhhqsaahap
      481 psshhvvaqa hhangsakhh q
//
LOCUS       XP_034182838             489 aa            linear   INV 03-SEP-2025
DEFINITION  tektin A [Osmia lignaria lignaria].
ACCESSION   XP_034182838
VERSION     XP_034182838.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326947.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034182838.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..489
                     /product="tektin A"
                     /calculated_mol_wt=55598
     Region          99..481
                     /region_name="Tektin"
                     /note="Tektin family; pfam03148"
                     /db_xref="CDD:460827"
     CDS             1..489
                     /gene="Tektin-A"
                     /coded_by="XM_034326947.2:127..1596"
                     /db_xref="GeneID:117605525"
ORIGIN      
        1 mdikqdsare tlqetsnngg gdtraipcpm mestdnaaek pppylpqpgd elpakldaqm
       61 daigpwatgr vkytpedglt gvrpvadrys itqfgapqwr annmrffqqs nekiqdaqla
      121 iskarrcvdq syreadkiql qstdhlktra glvyrwkidl ehvlkditee iesleaehrr
      181 vkqslsvlti pesiageflq lrskrlesdl irdeveeelt kevalcseir dllgrtrdqi
      241 emqltelkaa kvrlevdwtd ktdaydidae ciklkndspl ilwkpgatrf pgeqstpssy
      301 ehftreslsq getarqrsin lrstldaiyk nsikdlrdqa trvdvalgek iklteevcqq
      361 lekellhcly elantekaie elrrstrgld camkvaqtrl anrlqrrnve scrdtpqfal
      421 idevkllner tsamlaelkr aeetqaglvk arsdlereii vkrktlyidk ergqmlrsfy
      481 psaaalsgf
//
LOCUS       XP_076548175             412 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605626 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548175
VERSION     XP_076548175.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692060.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..412
                     /product="uncharacterized protein LOC117605626 isoform X1"
                     /calculated_mol_wt=47431
     Region          59..377
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..412
                     /gene="LOC117605626"
                     /coded_by="XM_076692060.1:1..1239"
                     /db_xref="GeneID:117605626"
ORIGIN      
        1 mdaatlekry lkvtkrfvkl sgiwpsetkf skylarfiin livvpalvtq aakvvcffsm
       61 nvavedipfm taligiltkq wtyivkekkf kllldgiyed waidrskeel nimttyairg
      121 afltsfyvgn ayfcvivfis qphisrifdk itshnesrnr lfiypayyai deekyyyait
      181 ahmssavisa llvysacdtm ymflvqhacs llavagyrfk haidfsidek gknvdtpnei
      241 hrkvcyavqg heranlylke ienihfhyll ismmlvmlgv tvtlfkisvm kitsefykyc
      301 sfliiqlmhi fflsiqgqfl anvndeiynn iyeamwynap vktqalyvla lrraltplql
      361 taggfvtmnl qsfadviiff tktendllnh llfsgnqnfr fvfygvgeca mn
//
LOCUS       XP_034183048             391 aa            linear   INV 03-SEP-2025
DEFINITION  odorant receptor 13a-like isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183048
VERSION     XP_034183048.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327157.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..391
                     /product="odorant receptor 13a-like isoform X2"
                     /calculated_mol_wt=44931
     Region          59..381
                     /region_name="7tm_6"
                     /note="7tm Odorant receptor; pfam02949"
                     /db_xref="CDD:251636"
     CDS             1..391
                     /gene="LOC117605626"
                     /coded_by="XM_034327157.2:1..1176"
                     /db_xref="GeneID:117605626"
ORIGIN      
        1 mdaatlekry lkvtkrfvkl sgiwpsetkf skylarfiin livvpalvtq aakvvcffsm
       61 nvavedipfm taligiltkq wtyivkekkf kllldgiyed waidrskeel nimttyairg
      121 afltsfyvgn ayfcvivfis qphisrifdk itshnesrnr lfiypayyai deekyyyait
      181 ahmssavisa llvysacdtm ymflvqhacs llavagyrfk haidfsidek gknvdtpnei
      241 hrkvcyavqg heranlylke ienihfhyll ismmlvmlgv tvtlfkisvm kitsefykyc
      301 sfliiqlmhi fflsiqgqfl anvndeiynn iyeamwynap vktqalyvla lrraltplql
      361 taggfvtmnl qsfadvikts vsyftvlesv q
//
LOCUS       XP_034182839             397 aa            linear   INV 03-SEP-2025
DEFINITION  thioredoxin domain-containing protein pretaporter [Osmia lignaria
            lignaria].
ACCESSION   XP_034182839
VERSION     XP_034182839.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326948.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..397
                     /product="thioredoxin domain-containing protein
                     pretaporter"
                     /calculated_mol_wt=45326
     Region          35..390
                     /region_name="ER_PDI_fam"
                     /note="protein disulfide isomerase, eukaryotic; TIGR01130"
                     /db_xref="CDD:273457"
     Region          160..260
                     /region_name="PDI_a_ERp46"
                     /note="PDIa family, endoplasmic reticulum protein 46
                     (ERp46) subfamily; ERp46 is an ER-resident protein
                     containing three redox active TRX domains. Yeast
                     complementation studies show that ERp46 can substitute for
                     protein disulfide isomerase (PDI) function in...; cd03005"
                     /db_xref="CDD:239303"
     Site            order(187,190,251)
                     /site_type="active"
                     /note="catalytic residues [active]"
                     /db_xref="CDD:239303"
     CDS             1..397
                     /gene="prtp"
                     /coded_by="XM_034326948.1:327..1520"
                     /db_xref="GeneID:117605526"
ORIGIN      
        1 mstyllnfim skkhlfliif mlsqvsseqe ddhvhtvqyt kdnfsteikk knhfvmfyap
       61 wcghcqrlep tweqlaeisn eddnnikiak vdctidstlc tehdvtgypt lkffkagesr
      121 gtkfrgtrdl pslisflndq lgttlgsndv apsppeavng lleltedtfd ihvstgyhfi
      181 kfyapwcghc qklaptweel anslrnnnyv siskvdctqh rnvcrqfdik gyptllwied
      241 gkkvdkysgq rtheelkayv skmlgkendq invkvdnsds ashtvllslt gesfkhgien
      301 gisfvkffap wcghckrlap iwedlgkkfl tnedvhiikv dctldaskel cneqevdgfp
      361 tlylyrdglk vaeyngarnl ddlyefvesy lqphdel
//
LOCUS       XP_076548176             293 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605627 [Osmia lignaria lignaria].
ACCESSION   XP_076548176
VERSION     XP_076548176.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692061.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="uncharacterized protein LOC117605627"
                     /calculated_mol_wt=30073
     Region          137..191
                     /region_name="LIM1_LMO4"
                     /note="The first LIM domain of LMO4 (LIM domain only
                     protein 4); cd09386"
                     /db_xref="CDD:188772"
     Site            order(137,140,158,161,164,167,187,190)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188772"
     Site            order(138..141,165..166,182..184)
                     /site_type="other"
                     /note="Lbd1 binding interface"
                     /db_xref="CDD:188772"
     Region          201..255
                     /region_name="LIM2_LMO4"
                     /note="The second LIM domain of LMO4 (LIM domain only
                     protein 4); cd09387"
                     /db_xref="CDD:188773"
     Site            order(201,204,223,226,229,232,251,254)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188773"
     CDS             1..293
                     /gene="LOC117605627"
                     /coded_by="XM_076692061.1:1174..2055"
                     /db_xref="GeneID:117605627"
ORIGIN      
        1 mnphhpghsa ayphhpslss sphhsgphhg ystggggggg ggggttttpd lpsphspphg
       61 paidpatpya smqpgqgmgs nghhhggagm msdhphhpgh phphlpghta yppvnhnnnc
      121 tlkqeggpsg ptgiqqcagc ggqiverwll famdrywhng clkcsycsaa laeighscyt
      181 rsgmilcktd yrrmfgssga cagcgnaipa selvmraggs vfhqkcftcs kcgsqlvsgd
      241 ryyllsgapv cetdwhkiik sssvaaaaaa aaagnggapv rkgkvgrprr sre
//
LOCUS       XP_034183049             293 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605627 [Osmia lignaria lignaria].
ACCESSION   XP_034183049
VERSION     XP_034183049.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327158.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..293
                     /product="uncharacterized protein LOC117605627"
                     /calculated_mol_wt=30073
     Region          137..191
                     /region_name="LIM1_LMO4"
                     /note="The first LIM domain of LMO4 (LIM domain only
                     protein 4); cd09386"
                     /db_xref="CDD:188772"
     Site            order(137,140,158,161,164,167,187,190)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188772"
     Site            order(138..141,165..166,182..184)
                     /site_type="other"
                     /note="Lbd1 binding interface"
                     /db_xref="CDD:188772"
     Region          201..255
                     /region_name="LIM2_LMO4"
                     /note="The second LIM domain of LMO4 (LIM domain only
                     protein 4); cd09387"
                     /db_xref="CDD:188773"
     Site            order(201,204,223,226,229,232,251,254)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188773"
     CDS             1..293
                     /gene="LOC117605627"
                     /coded_by="XM_034327158.2:731..1612"
                     /db_xref="GeneID:117605627"
ORIGIN      
        1 mnphhpghsa ayphhpslss sphhsgphhg ystggggggg ggggttttpd lpsphspphg
       61 paidpatpya smqpgqgmgs nghhhggagm msdhphhpgh phphlpghta yppvnhnnnc
      121 tlkqeggpsg ptgiqqcagc ggqiverwll famdrywhng clkcsycsaa laeighscyt
      181 rsgmilcktd yrrmfgssga cagcgnaipa selvmraggs vfhqkcftcs kcgsqlvsgd
      241 ryyllsgapv cetdwhkiik sssvaaaaaa aaagnggapv rkgkvgrprr sre
//
LOCUS       XP_034183056             106 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605629 [Osmia lignaria lignaria].
ACCESSION   XP_034183056
VERSION     XP_034183056.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327165.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..106
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..106
                     /product="uncharacterized protein LOC117605629"
                     /calculated_mol_wt=12003
     CDS             1..106
                     /gene="LOC117605629"
                     /coded_by="XM_034327165.2:89..409"
                     /db_xref="GeneID:117605629"
ORIGIN      
        1 mrmesvraia asaifkitda rvringdrkr slrvnrrhel partssfgev eqrktkgerf
       61 irvlhlhqpl sklhatdeaa lmklslrtsv vdvktfqslp aktltr
//
LOCUS       XP_034182841              94 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa [Osmia
            lignaria lignaria].
ACCESSION   XP_034182841
VERSION     XP_034182841.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034326950.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..94
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..94
                     /product="NADH dehydrogenase (ubiquinone) 1 beta
                     subcomplex, 2, 8kDa"
                     /calculated_mol_wt=11106
     Region          28..93
                     /region_name="NADH_B2"
                     /note="NADH dehydrogenase 1 beta subcomplex subunit 2;
                     pfam14813"
                     /db_xref="CDD:464329"
     CDS             1..94
                     /gene="Ndufb2"
                     /coded_by="XM_034326950.2:90..374"
                     /db_xref="GeneID:117605528"
ORIGIN      
        1 mllsrsaril knayqlkghk nvavnlnrsh yewsyrsvpp rdtkwvitte ivagigwwwi
       61 lysfwhhsdh vfgefeyphp ddwtdeelgi ppde
//
LOCUS       XP_034183029             479 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605613 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183029
VERSION     XP_034183029.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327138.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183029.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..479
                     /product="uncharacterized protein LOC117605613 isoform X1"
                     /calculated_mol_wt=54868
     Region          <124..>365
                     /region_name="MCLC"
                     /note="Mid-1-related chloride channel (MCLC); pfam05934"
                     /db_xref="CDD:461783"
     CDS             1..479
                     /gene="LOC117605613"
                     /coded_by="XM_034327138.2:132..1571"
                     /db_xref="GeneID:117605613"
ORIGIN      
        1 mknfntfvyi tvhifalili vncedenyfe eeissdelid phsffydkqs kkminepelr
       61 mtdnskensn nirsnlnpsk eidnhssler ifykrlvnll lsniniqvkd evsiigilei
      121 eishsqmeil qnfhiqktsl reideilsnv ikkprtdyla gvmhvfdtil qkfetvlrtv
      181 qqhpdgamiv famfmvcltc rlikwgqgfp iflfiqvifv lsffmtwwql iqeaevkfaa
      241 aqmkftdipv vcqpnkmnmw dkfvsfistn edcekyyhak msnpklkitp afalshfitt
      301 vvlhpitymg tvvsgfitna tddlpwtyaw iikcilflcv glviiimpfc lsgasinlgl
      361 gpllrfgind qkksekgksl qnvekrepvq iilqvphatn vpmiqdvsni eqpkavkmlk
      421 ndddgnstdr edyfeklned lscgdtiknt efsetddksk tgkqhdtaki pkndgrgdg
//
LOCUS       XP_034183030             478 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605613 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183030
VERSION     XP_034183030.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327139.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183030.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..478
                     /product="uncharacterized protein LOC117605613 isoform X2"
                     /calculated_mol_wt=54781
     Region          <123..>364
                     /region_name="MCLC"
                     /note="Mid-1-related chloride channel (MCLC); pfam05934"
                     /db_xref="CDD:461783"
     CDS             1..478
                     /gene="LOC117605613"
                     /coded_by="XM_034327139.2:133..1569"
                     /db_xref="GeneID:117605613"
ORIGIN      
        1 mknfntfvyi tvhifalili vncedenyfe eeisdelidp hsffydkqsk kminepelrm
       61 tdnskensnn irsnlnpske idnhssleri fykrlvnlll sniniqvkde vsiigileie
      121 ishsqmeilq nfhiqktslr eideilsnvi kkprtdylag vmhvfdtilq kfetvlrtvq
      181 qhpdgamivf amfmvcltcr likwgqgfpi flfiqvifvl sffmtwwqli qeaevkfaaa
      241 qmkftdipvv cqpnkmnmwd kfvsfistne dcekyyhakm snpklkitpa falshfittv
      301 vlhpitymgt vvsgfitnat ddlpwtyawi ikcilflcvg lviiimpfcl sgasinlglg
      361 pllrfgindq kksekgkslq nvekrepvqi ilqvphatnv pmiqdvsnie qpkavkmlkn
      421 dddgnstdre dyfeklnedl scgdtiknte fsetddkskt gkqhdtakip kndgrgdg
//
LOCUS       XP_034183031             392 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605613 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183031
VERSION     XP_034183031.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327140.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183031.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..392
                     /product="uncharacterized protein LOC117605613 isoform X3"
                     /calculated_mol_wt=45329
     Region          <124..>365
                     /region_name="MCLC"
                     /note="Mid-1-related chloride channel (MCLC); pfam05934"
                     /db_xref="CDD:461783"
     CDS             1..392
                     /gene="LOC117605613"
                     /coded_by="XM_034327140.2:134..1312"
                     /db_xref="GeneID:117605613"
ORIGIN      
        1 mknfntfvyi tvhifalili vncedenyfe eeissdelid phsffydkqs kkminepelr
       61 mtdnskensn nirsnlnpsk eidnhssler ifykrlvnll lsniniqvkd evsiigilei
      121 eishsqmeil qnfhiqktsl reideilsnv ikkprtdyla gvmhvfdtil qkfetvlrtv
      181 qqhpdgamiv famfmvcltc rlikwgqgfp iflfiqvifv lsffmtwwql iqeaevkfaa
      241 aqmkftdipv vcqpnkmnmw dkfvsfistn edcekyyhak msnpklkitp afalshfitt
      301 vvlhpitymg tvvsgfitna tddlpwtyaw iikcilflcv glviiimpfc lsgasinlgl
      361 gpllrfgind qkksekgtsc yqrtndtrri ky
//
LOCUS       XP_034183035             239 aa            linear   INV 03-SEP-2025
DEFINITION  steroid receptor RNA activator 1 [Osmia lignaria lignaria].
ACCESSION   XP_034183035
VERSION     XP_034183035.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327144.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183035.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..239
                     /product="steroid receptor RNA activator 1"
                     /calculated_mol_wt=27049
     Region          90..215
                     /region_name="SRA1"
                     /note="Steroid receptor RNA activator (SRA1); pfam07304"
                     /db_xref="CDD:429397"
     CDS             1..239
                     /gene="LOC117605616"
                     /coded_by="XM_034327144.2:183..902"
                     /db_xref="GeneID:117605616"
ORIGIN      
        1 mqycfhrkns kelsltmnek eanqnlstqk vllpghdpgw ndppkwvytg aqssatpakr
       61 mlnkrvafpl astkandkes ssetkslnmp pllqpsnltt altkdvdvkv teikidkekv
      121 lsdvleklnt vinehilekh tieevrkrld ilksmwledk lnnviyknil dlstalqegn
      181 vekadqihvt lmmqyaslcs swipgirhii lelksrtqda datqpqnpes hlfsveenh
//
LOCUS       XP_034183189             604 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605689 [Osmia lignaria lignaria].
ACCESSION   XP_034183189
VERSION     XP_034183189.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327298.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..604
                     /product="uncharacterized protein LOC117605689"
                     /calculated_mol_wt=70024
     CDS             1..604
                     /gene="LOC117605689"
                     /coded_by="XM_034327298.2:16..1830"
                     /db_xref="GeneID:117605689"
ORIGIN      
        1 mreagrrsai stsqlglysd hsdrynrnry ssvtrkfaig vmvvvvvilv aiflydftsa
       61 tslnsklnqe tkhiyilqna lkkplnitkk fsistvspev nstnsirien rsdnenpgnp
      121 ffydetdeyk vpdaeirsqn lnnfklrkrv lksvenkeee aegkqlfdnh alvyrvrqry
      181 khpdsnedme sseesrpvpf hwelktpqpt sfkrqrypql sqyryphasr niqeiikylt
      241 ndadvpnrgi kftgvyvnpk kydlvpelgg mmsnsdrsee ddgsysvned pfyqykpkhp
      301 advnllatsn frfsptglhr ynydtygrpv nynkapspda qyensgsysn syskrrkpkp
      361 fsvmldiypi tdiveqnkki trprqvpved yelrrpiqyn rgpkfyaapp qsvtgmstqa
      421 mtdeeerqqm ifhlnlyprk knkmsrhdii hrsesmepee qqqfvnkvws pletiakhla
      481 verskfvdne mednlastry qettldnene tkkfdqmnds ssqndsipfn hksiieedyg
      541 stekyefeaq kivgtttesk cedcnttisl detttnetks aevskdvdti egfkrfsdsi
      601 sktn
//
LOCUS       XP_034186804            1176 aa            linear   INV 03-SEP-2025
DEFINITION  cell division cycle and apoptosis regulator protein 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034186804
VERSION     XP_034186804.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330913.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1176
                     /product="cell division cycle and apoptosis regulator
                     protein 1 isoform X1"
                     /calculated_mol_wt=135293
     Region          127..185
                     /region_name="S1-like"
                     /note="pfam14444"
                     /db_xref="CDD:464176"
     Region          325..407
                     /region_name="BURAN"
                     /note="BURAN domain; pfam19257"
                     /db_xref="CDD:466013"
     Region          431..554
                     /region_name="DBC1"
                     /note="pfam14443"
                     /db_xref="CDD:464175"
     Region          557..>660
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          598..630
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     Region          690..757
                     /region_name="LAIKA"
                     /note="LAIKA domain; pfam19256"
                     /db_xref="CDD:466012"
     Region          <938..>1084
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1176
                     /gene="LOC117607336"
                     /coded_by="XM_034330913.2:98..3628"
                     /db_xref="GeneID:117607336"
ORIGIN      
        1 msnlspfggg knppwvrnag qgiqniqqqm lgqamgsigg qpmvqyqqqt qqvyqqslgl
       61 qqpnitmasm atlgsnlpsg iagqlypqva tvsypppral ntnafqpsva gvpqqvqqnv
      121 pssstkqrvf tgtvtqvydn fgfvdedvff qtnacvkgsn pvvgdrvlve asynpsmpfk
      181 wsatriqvlp mgnnnsntnt qqsnqtsrqq qqqqqpqqsr tsgtynavpp paenannrfa
      241 tsatsantas nrnkigrvre rsprerknee eeierkrrre erirerekke erspsrtrrs
      301 ksprprrrtr vvprymvqip kialdlpead vleirrryqn myipsdffst nfrwvdafpp
      361 hmpftlnkpc sfhvmhkdid pctentavle psdadylfsa kvmlismpam eeiykrccgv
      421 sedrdpdrdy vhptrlinfl vglrgknetm aiggpwspsl dgpnpekdps vlirtavrtc
      481 kaltgidlss ctqwyrflel yyrraetthk sgrvvpsrve tvilflpdvw scvptklewd
      541 glqlsykkql erkllrttss pddsdaandt deaavadqke dpvpekkdpt hyseldpksm
      601 nvnelrqela arnlnckglk sqllarltka atleqakeeg rqddieenek dvspspkeee
      661 dkkfrdkdhd edrrklcere rialekrytl pesshiivhp srmaksgkfd ctvmslsvll
      721 dyrpedtkeh sfevslfael fnemlmrdfg friyralcsl pekpkekded kkkdkkddkr
      781 dekkddkrkd ddkrcrkder kdekrregsk dkkdekdaks ktddrdrdke knddddddee
      841 desddddsvk dgrrdrdkdg rkrktklyth dpylllsfvy fdqthcgyif dkdieeliyt
      901 lglnlsraqv rklvqkvvtr dslhyrkltd rsrdddpkee kkddketdkn dsakedyeed
      961 ilrslalgnk kllpvfvgsg ppskrarred sladqseest ipegfvmykg slvdveklvs
     1021 qlkrsekarl dteermmelq helsivneks akqtnnikal sedlkiykdk lrntdeklkk
     1081 vsaeclsylt avknmyhiaa kmmqsdtkkv eiveiqdekv gevngseiet kfktdtrwgd
     1141 nkvpikkefi etdkdkkcdn kisikkeite idkekk
//
LOCUS       XP_034186805            1164 aa            linear   INV 03-SEP-2025
DEFINITION  cell division cycle and apoptosis regulator protein 1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034186805
VERSION     XP_034186805.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330914.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1164
                     /product="cell division cycle and apoptosis regulator
                     protein 1 isoform X2"
                     /calculated_mol_wt=133953
     Region          115..173
                     /region_name="S1-like"
                     /note="pfam14444"
                     /db_xref="CDD:464176"
     Region          313..395
                     /region_name="BURAN"
                     /note="BURAN domain; pfam19257"
                     /db_xref="CDD:466013"
     Region          419..542
                     /region_name="DBC1"
                     /note="pfam14443"
                     /db_xref="CDD:464175"
     Region          545..>648
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     Region          586..618
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     Region          678..745
                     /region_name="LAIKA"
                     /note="LAIKA domain; pfam19256"
                     /db_xref="CDD:466012"
     Region          <926..>1072
                     /region_name="SMC_N"
                     /note="RecF/RecN/SMC N terminal domain; cl47134"
                     /db_xref="CDD:481474"
     CDS             1..1164
                     /gene="LOC117607336"
                     /coded_by="XM_034330914.2:98..3592"
                     /db_xref="GeneID:117607336"
ORIGIN      
        1 msnlspfggg knppwvrnag qamgsiggqp mvqyqqqtqq vyqqslglqq pnitmasmat
       61 lgsnlpsgia gqlypqvatv sypppralnt nafqpsvagv pqqvqqnvps sstkqrvftg
      121 tvtqvydnfg fvdedvffqt nacvkgsnpv vgdrvlveas ynpsmpfkws atriqvlpmg
      181 nnnsntntqq snqtsrqqqq qqqpqqsrts gtynavpppa enannrfats atsantasnr
      241 nkigrvrers prerkneeee ierkrrreer irerekkeer spsrtrrsks prprrrtrvv
      301 prymvqipki aldlpeadvl eirrryqnmy ipsdffstnf rwvdafpphm pftlnkpcsf
      361 hvmhkdidpc tentavleps dadylfsakv mlismpamee iykrccgvse drdpdrdyvh
      421 ptrlinflvg lrgknetmai ggpwspsldg pnpekdpsvl irtavrtcka ltgidlssct
      481 qwyrflelyy rraetthksg rvvpsrvetv ilflpdvwsc vptklewdgl qlsykkqler
      541 kllrttsspd dsdaandtde aavadqkedp vpekkdpthy seldpksmnv nelrqelaar
      601 nlnckglksq llarltkaat leqakeegrq ddieenekdv spspkeeedk kfrdkdhded
      661 rrklcereri alekrytlpe sshiivhpsr maksgkfdct vmslsvlldy rpedtkehsf
      721 evslfaelfn emlmrdfgfr iyralcslpe kpkekdedkk kdkkddkrde kkddkrkddd
      781 krcrkderkd ekrregskdk kdekdakskt ddrdrdkekn ddddddeede sddddsvkdg
      841 rrdrdkdgrk rktklythdp ylllsfvyfd qthcgyifdk dieeliytlg lnlsraqvrk
      901 lvqkvvtrds lhyrkltdrs rdddpkeekk ddketdknds akedyeedil rslalgnkkl
      961 lpvfvgsgpp skrarredsl adqseestip egfvmykgsl vdveklvsql krsekarldt
     1021 eermmelqhe lsivneksak qtnnikalse dlkiykdklr ntdeklkkvs aeclsyltav
     1081 knmyhiaakm mqsdtkkvei veiqdekvge vngseietkf ktdtrwgdnk vpikkefiet
     1141 dkdkkcdnki sikkeiteid kekk
//
LOCUS       XP_034186806            1093 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 1
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186806
VERSION     XP_034186806.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330915.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186806.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1093
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 1 isoform X1"
                     /calculated_mol_wt=124454
     Region          270..466
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(405..406,409,415)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          488..552
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          573..624
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          <667..733
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          870..925
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          929..998
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          1002..1049
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     CDS             1..1093
                     /gene="LOC117607337"
                     /coded_by="XM_034330915.2:710..3991"
                     /db_xref="GeneID:117607337"
ORIGIN      
        1 mtqsklpsfl ltmwllgtia tksrmsdflq slqeyeivyp riipngklrn rrstadhvee
       61 pvfleinnwt lrtissdrli lssnftvdwv hsnkmdptsq avqgncnlrq gnlegdkssl
      121 avvtlceqdv yalmmidrks ffvqplnngq hvmyrseqtg wwsskddpni vslrrgerse
      181 vkerkrrhsa drynqefynl sgdvfdneyp eaerlilyde ndnvsseynd timeqltvda
      241 daeeidyfsg stwhrstskk ktvnkslppr wlelgiaady svtdfhgvrv qqyimallni
      301 vsaiymdpsl esnmilvivr milytekrdg rvcrgdarrs lenvnrwnkk mlsstdirhd
      361 vavwltrldi ggpsgyapvs gvcdparsca lnrdegltsa fiiaheiahi lglthdgdes
      421 ngnackeess qgsvmapmva atfhhfywst csknefhrrv rrrsclsnkp kyegskqlka
      481 afretgtfsm deqcriefge gyeycktfdv tepcsrlwcg nsnisetckt kkgpplegtv
      541 cgvskwcing rcqsnrrnld lgivlnkprd ggwsawsdwg kcsrtcgigv qcrtrkcnkp
      601 lpihggkycs gptddcklcd lpkclipvdl raqqcskltd tlllegilsk ynvtwlpyes
      661 eqenfkcrli crsretgeif dtrrnlidgt pcsygstdic iqgechqmgc dnvlgskkvf
      721 dvcgvcdgdh sncesvmskf qrklrrevtr laiipreayn lslnvtilps sffnpnnlti
      781 iigdgrrrrn iqkhltfrkk dliiaqgaaf riqrrddnty svrvdgttfe dvvillvtpq
      841 svteqtasvt asleyfinrd krnsknrykw lfggwsfcst scgngtrhki ivckdeqtgk
      901 ivsrrkcplm tkpsqeiekc ngfsctfkwl pgpwegcstt cgsygmqlrq lycvhsdfsg
      961 tevnkdnele vyrtmvqpsv cqttsmptnt recnripcpg rwvftewssc snnngkgiks
     1021 riarcippen esfytcdgpt ikteirsctn hitraiefpy rcwseesrfc sipslkrycn
     1081 vpafrrrccr tcd
//
LOCUS       XP_076548178             941 aa            linear   INV 03-SEP-2025
DEFINITION  A disintegrin and metalloproteinase with thrombospondin motifs 3
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548178
VERSION     XP_076548178.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692063.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..941
                     /product="A disintegrin and metalloproteinase with
                     thrombospondin motifs 3 isoform X2"
                     /calculated_mol_wt=107080
     Region          118..314
                     /region_name="ZnMc_ADAMTS_like"
                     /note="Zinc-dependent metalloprotease, ADAMTS_like
                     subgroup. ADAMs (A Disintegrin And Metalloprotease) are
                     glycoproteins, which play roles in cell signaling, cell
                     fusion, and cell-cell interactions. This particular
                     subfamily represents domain architectures that...;
                     cd04273"
                     /db_xref="CDD:239801"
     Site            order(253..254,257,263)
                     /site_type="active"
                     /db_xref="CDD:239801"
     Region          336..400
                     /region_name="ADAMTS_CR_2"
                     /note="ADAMTS cysteine-rich domain 2; pfam17771"
                     /db_xref="CDD:465496"
     Region          421..472
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     Region          <515..581
                     /region_name="ADAMTS_CR_3"
                     /note="ADAMTS cysteine-rich domain; pfam19236"
                     /db_xref="CDD:437068"
     Region          718..773
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          777..846
                     /region_name="TSP1_ADAMTS"
                     /note="Thrombospondin type 1 domain; pfam19030"
                     /db_xref="CDD:465950"
     Region          850..897
                     /region_name="TSP1"
                     /note="Thrombospondin type 1 repeats; smart00209"
                     /db_xref="CDD:214559"
     CDS             1..941
                     /gene="LOC117607337"
                     /coded_by="XM_076692063.1:175..3000"
                     /db_xref="GeneID:117607337"
ORIGIN      
        1 myrseqtgww sskddpnivs lrrgersevk erkrrhsadr ynqefynlsg dvfdneypea
       61 erlilydend nvsseyndti meqltvdada eeidyfsgst whrstskkkt vnkslpprwl
      121 elgiaadysv tdfhgvrvqq yimallnivs aiymdpsles nmilvivrmi lytekrdgrv
      181 crgdarrsle nvnrwnkkml sstdirhdva vwltrldigg psgyapvsgv cdparscaln
      241 rdegltsafi iaheiahilg lthdgdesng nackeessqg svmapmvaat fhhfywstcs
      301 knefhrrvrr rsclsnkpky egskqlkaaf retgtfsmde qcriefgegy eycktfdvte
      361 pcsrlwcgns nisetcktkk gpplegtvcg vskwcingrc qsnrrnldlg ivlnkprdgg
      421 wsawsdwgkc srtcgigvqc rtrkcnkplp ihggkycsgp tddcklcdlp kclipvdlra
      481 qqcskltdtl llegilskyn vtwlpyeseq enfkcrlicr sretgeifdt rrnlidgtpc
      541 sygstdiciq gechqmgcdn vlgskkvfdv cgvcdgdhsn cesvmskfqr klrrevtrla
      601 iipreaynls lnvtilpssf fnpnnltiii gdgrrrrniq khltfrkkdl iiaqgaafri
      661 qrrddntysv rvdgttfedv villvtpqsv teqtasvtas leyfinrdkr nsknrykwlf
      721 ggwsfcstsc gngtrhkiiv ckdeqtgkiv srrkcplmtk psqeiekcng fsctfkwlpg
      781 pwegcsttcg sygmqlrqly cvhsdfsgte vnkdnelevy rtmvqpsvcq ttsmptntre
      841 cnripcpgrw vftewsscsn nngkgiksri arcippenes fytcdgptik teirsctnhi
      901 traiefpyrc wseesrfcsi pslkrycnvp afrrrccrtc d
//
LOCUS       XP_034186809            1070 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607338 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186809
VERSION     XP_034186809.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330918.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186809.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1070
                     /product="uncharacterized protein LOC117607338 isoform X1"
                     /calculated_mol_wt=116825
     CDS             1..1070
                     /gene="LOC117607338"
                     /coded_by="XM_034330918.2:316..3528"
                     /db_xref="GeneID:117607338"
ORIGIN      
        1 mgqcvsrkag aviatghrds ltyrimdkpa kgehkgaskr ggshtrgtam sfgfrrrpts
       61 gipvpktslp ielsmlhprs ksagpeqnrr rdedtniihn sssgrstprl appkkeasgi
      121 avrtnrfgyr qsqakftnkv gdirsshsvs hynheklqqq qqqqegfvkq phrvqvssvp
      181 apkgkpspvh npnsynntni nhsdgnrrts sipepigryt lhtshlplpq favrmtdans
      241 kiaktaanqs rkvsatskss tsskegsgte dsgvgsqqgc pgendlrsvd ytdgslsrrr
      301 gvgrprnlrm vvngksfdvr dvrdddstvt eisviplpkt faaastglvr erttqyqriv
      361 nkdnrytest tsmsttsseg ydeglgeekv ykdrshseki psiksdfspp ssddpeyghg
      421 eamadeysls ssdecqrsts aqhlqtstdt akngtvpkta lrsvlltied pafaaaaats
      481 taliddetsp vdslfdslta sitqsdakap kkeyameqsg ntidddspgt ptnvsnslsl
      541 segreyfdde iadqpglvfd dnsraggetq samasqavte nshtlvessp ktdyvnellp
      601 slgtghgkna tnsphhgkri sragsvdtls pcesiasddl ildyeqsdas syeeqqqrve
      661 sntalqdmdd atilseleaq geevmrqwts llgtcqmlqq tnnsntvnnn vnggstnnnn
      721 qpaneignec dkttkvwrnr svtdtprsvd nlrnrqfssp lrtsrnvhsp sldsgdegsl
      781 rvdrntyqhm fqdivsiktm llklkrvlqe seengltrte tlnpfdnsmk nglfynlneg
      841 ntadvstspg sggnsiadel adlrrqvvfl qgqvedrdrt iqvlqfqisk lqgpngndgq
      901 tcalasnhnn vnpdtcnaat qtektrpvsa gpslvqtppq ddamwplirw sdswdqhrpt
      961 llgeqltsnr slrkstdrip rtiksrqeet shrrnghidk stvdsnssng kilklkdsks
     1021 iesngteqnd iktegsptks ciptsrklat pvlitrstra vtstgrshkt
//
LOCUS       XP_034186810            1070 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607338 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186810
VERSION     XP_034186810.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330919.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186810.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1070
                     /product="uncharacterized protein LOC117607338 isoform X1"
                     /calculated_mol_wt=116825
     CDS             1..1070
                     /gene="LOC117607338"
                     /coded_by="XM_034330919.2:258..3470"
                     /db_xref="GeneID:117607338"
ORIGIN      
        1 mgqcvsrkag aviatghrds ltyrimdkpa kgehkgaskr ggshtrgtam sfgfrrrpts
       61 gipvpktslp ielsmlhprs ksagpeqnrr rdedtniihn sssgrstprl appkkeasgi
      121 avrtnrfgyr qsqakftnkv gdirsshsvs hynheklqqq qqqqegfvkq phrvqvssvp
      181 apkgkpspvh npnsynntni nhsdgnrrts sipepigryt lhtshlplpq favrmtdans
      241 kiaktaanqs rkvsatskss tsskegsgte dsgvgsqqgc pgendlrsvd ytdgslsrrr
      301 gvgrprnlrm vvngksfdvr dvrdddstvt eisviplpkt faaastglvr erttqyqriv
      361 nkdnrytest tsmsttsseg ydeglgeekv ykdrshseki psiksdfspp ssddpeyghg
      421 eamadeysls ssdecqrsts aqhlqtstdt akngtvpkta lrsvlltied pafaaaaats
      481 taliddetsp vdslfdslta sitqsdakap kkeyameqsg ntidddspgt ptnvsnslsl
      541 segreyfdde iadqpglvfd dnsraggetq samasqavte nshtlvessp ktdyvnellp
      601 slgtghgkna tnsphhgkri sragsvdtls pcesiasddl ildyeqsdas syeeqqqrve
      661 sntalqdmdd atilseleaq geevmrqwts llgtcqmlqq tnnsntvnnn vnggstnnnn
      721 qpaneignec dkttkvwrnr svtdtprsvd nlrnrqfssp lrtsrnvhsp sldsgdegsl
      781 rvdrntyqhm fqdivsiktm llklkrvlqe seengltrte tlnpfdnsmk nglfynlneg
      841 ntadvstspg sggnsiadel adlrrqvvfl qgqvedrdrt iqvlqfqisk lqgpngndgq
      901 tcalasnhnn vnpdtcnaat qtektrpvsa gpslvqtppq ddamwplirw sdswdqhrpt
      961 llgeqltsnr slrkstdrip rtiksrqeet shrrnghidk stvdsnssng kilklkdsks
     1021 iesngteqnd iktegsptks ciptsrklat pvlitrstra vtstgrshkt
//
LOCUS       XP_034186811            1063 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607338 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186811
VERSION     XP_034186811.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330920.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186811.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1063
                     /product="uncharacterized protein LOC117607338 isoform X2"
                     /calculated_mol_wt=116096
     CDS             1..1063
                     /gene="LOC117607338"
                     /coded_by="XM_034330920.2:432..3623"
                     /db_xref="GeneID:117607338"
ORIGIN      
        1 mdyrstgtrc eddtlenekg igaagehkga skrggshtrg tamsfgfrrr ptsgipvpkt
       61 slpielsmlh prsksagpeq nrrrdedtni ihnsssgrst prlappkkea sgiavrtnrf
      121 gyrqsqakft nkvgdirssh svshynhekl qqqqqqqegf vkqphrvqvs svpapkgkps
      181 pvhnpnsynn tninhsdgnr rtssipepig rytlhtshlp lpqfavrmtd anskiaktaa
      241 nqsrkvsats ksstsskegs gtedsgvgsq qgcpgendlr svdytdgsls rrrgvgrprn
      301 lrmvvngksf dvrdvrddds tvteisvipl pktfaaastg lvrerttqyq rivnkdnryt
      361 esttsmstts segydeglge ekvykdrshs ekipsiksdf sppssddpey ghgeamadey
      421 slsssdecqr stsaqhlqts tdtakngtvp ktalrsvllt iedpafaaaa atstalidde
      481 tspvdslfds ltasitqsda kapkkeyame qsgntiddds pgtptnvsns lslsegreyf
      541 ddeiadqpgl vfddnsragg etqsamasqa vtenshtlve sspktdyvne llpslgtghg
      601 knatnsphhg krisragsvd tlspcesias ddlildyeqs dassyeeqqq rvesntalqd
      661 mddatilsel eaqgeevmrq wtsllgtcqm lqqtnnsntv nnnvnggstn nnnqpaneig
      721 necdkttkvw rnrsvtdtpr svdnlrnrqf ssplrtsrnv hspsldsgde gslrvdrnty
      781 qhmfqdivsi ktmllklkrv lqeseenglt rtetlnpfdn smknglfynl negntadvst
      841 spgsggnsia deladlrrqv vflqgqvedr drtiqvlqfq isklqgpngn dgqtcalasn
      901 hnnvnpdtcn aatqtektrp vsagpslvqt ppqddamwpl irwsdswdqh rptllgeqlt
      961 snrslrkstd riprtiksrq eetshrrngh idkstvdsns sngkilklkd sksiesngte
     1021 qndiktegsp tksciptsrk latpvlitrs travtstgrs hkt
//
LOCUS       XP_034186812            1062 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607338 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186812
VERSION     XP_034186812.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330921.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186812.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1062
                     /product="uncharacterized protein LOC117607338 isoform X3"
                     /calculated_mol_wt=115939
     CDS             1..1062
                     /gene="LOC117607338"
                     /coded_by="XM_034330921.2:317..3505"
                     /db_xref="GeneID:117607338"
ORIGIN      
        1 mgqcvsrkag aviatghrds ltyrimdkpa kgehkgaskr ggshtrgtam sfgfrrrpts
       61 gipvpktslp ielsmlhprs ksagpeqnrr rdedtniihn sssgrstprl appkkeasgi
      121 avrtnrfgyr qsqakftnkv gdirsshsvs hynheklqqq qqqqegfvkq phrvqvssvp
      181 apkgkpspvh npnsynntni nhsdgnrrts sipepigryt lhtshlplpq favrmtdans
      241 kiaktaanqs rkvsatskss tsskegsgte dsgvgsqqgc pgendlrsvd ytdgslsrrr
      301 gvgrprnlrm vvngksfdvr dvrdddstvt eisviplpkt faaastglvr erttqyqriv
      361 nkdnrytest tsmsttsseg ydeglgeekv ykdrshseki psiksdfspp ssddpeyghg
      421 eamadeysls ssdecqrsts aqhlqtstdt akngtvpkta lrsvlltied pafaaaaats
      481 taliddetsp vdslfdslta sitqsdakap kkeyameqsg ntidddspgt ptnvsnslsl
      541 segreyfdde iadqpglvfd dnsraggetq samasqavte nshtlvessp ktdyvnellp
      601 slgtghgkna tnsphhgkri sragsvdtls pcesiasddl ildyeqsdas syeeqqqrve
      661 sntalqdmdd atilseleaq geevmrqwts llgtcqmlqq tnnsntvnnn vnggstnnnn
      721 qpaneignec dkttkvwrnr svtdtprsvd nlrnrqfssp lrtsrnvhsp sldsgdegsl
      781 rvdrntyqhm fqdivsiktm llklkrvlqe tetlnpfdns mknglfynln egntadvsts
      841 pgsggnsiad eladlrrqvv flqgqvedrd rtiqvlqfqi sklqgpngnd gqtcalasnh
      901 nnvnpdtcna atqtektrpv sagpslvqtp pqddamwpli rwsdswdqhr ptllgeqlts
      961 nrslrkstdr iprtiksrqe etshrrnghi dkstvdsnss ngkilklkds ksiesngteq
     1021 ndiktegspt ksciptsrkl atpvlitrst ravtstgrsh kt
//
LOCUS       XP_034186813            1060 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607338 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186813
VERSION     XP_034186813.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330922.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186813.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1060
                     /product="uncharacterized protein LOC117607338 isoform X4"
                     /calculated_mol_wt=115681
     CDS             1..1060
                     /gene="LOC117607338"
                     /coded_by="XM_034330922.2:311..3493"
                     /db_xref="GeneID:117607338"
ORIGIN      
        1 mgqcvsrkag aviatghrds ltyrimdkpa kgehkgaskr ggshtrgtam sfgfrrrpts
       61 gipvpktslp ielsmlhprs ksagpeqnrr rdedtniihn sssgrstprl appkkeasgi
      121 avrtnrfgyr qsqakftnkv gdirsshsvs hynheklqqq qqqqegfvkq phrvqvssvp
      181 apkgkpspvh npnsynntni nhsdgnrrts sipepigryt lhtshlplpq favrmtdans
      241 kiaktaanqs rkvsatskss tsskegsgte dsgvgsqqgc pgendlrsvd ytdgslsrrr
      301 gvgrprnlrm vvngksfdvr dvrdddstvt eisviplpkt faaastglvr erttqyqriv
      361 nkdnrytest tsmsttsseg ydeglgeekv ykdrshseki psiksdfspp ssddpeyghg
      421 eamadeysls ssdecqrsts aqhlqtstdt akngtvpkta lrsvlltied pafaaaaats
      481 taliddetsp vdslfdslta sitqsdakap kkeyameqsg ntidddspgt ptnvsnslsl
      541 segreyfdde iadqpglvfd dnsraggetq samasqavte nshtlvessp ktgtghgkna
      601 tnsphhgkri sragsvdtls pcesiasddl ildyeqsdas syeeqqqrve sntalqdmdd
      661 atilseleaq geevmrqwts llgtcqmlqq tnnsntvnnn vnggstnnnn qpaneignec
      721 dkttkvwrnr svtdtprsvd nlrnrqfssp lrtsrnvhsp sldsgdegsl rvdrntyqhm
      781 fqdivsiktm llklkrvlqe seengltrte tlnpfdnsmk nglfynlneg ntadvstspg
      841 sggnsiadel adlrrqvvfl qgqvedrdrt iqvlqfqisk lqgpngndgq tcalasnhnn
      901 vnpdtcnaat qtektrpvsa gpslvqtppq ddamwplirw sdswdqhrpt llgeqltsnr
      961 slrkstdrip rtiksrqeet shrrnghidk stvdsnssng kilklkdsks iesngteqnd
     1021 iktegsptks ciptsrklat pvlitrstra vtstgrshkt
//
LOCUS       XP_076548179             959 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607338 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548179
VERSION     XP_076548179.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692064.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..959
                     /product="uncharacterized protein LOC117607338 isoform X5"
                     /calculated_mol_wt=104411
     CDS             1..959
                     /gene="LOC117607338"
                     /coded_by="XM_076692064.1:320..3199"
                     /db_xref="GeneID:117607338"
ORIGIN      
        1 mgqcvsrkag aviatghrds ltyrimdkpa kgehkgaskr ggshtrgtam sfgfrrrpts
       61 gipvpktslp ielsmlhprs ksagpeqnrr rdedtniihn sssgrstprl appkkeasgi
      121 avrtnrfgyr qsqakftnkv gdirsshsvs hynheklqqq qqqqegfvkq phrvqvssvp
      181 apkgkpspvh npnsynntni nhsdgnrrts sipepigryt lhtshlplpq favrmtdans
      241 kiaktaanqs rkvsatskss tsskegsgte dsgvgsqqgc pgendlrsvd ytdgslsrrr
      301 gvgrprnlrm vvngksfdvr dvrdddstvt eisviplpkt faaastglvr erttqyqriv
      361 nkdnrytest tsmsttsseg ydeglgeekv ykdrshseki psiksdfspp ssddpeyghg
      421 eamadeysls ssdecqrsts aqhlqtstdt akngtvpkta lrsvlltied pafaaaaats
      481 taliddetsp vdslfdslta sitqsdakap kkeyameqsg ntidddspgt ptnvsnslsl
      541 segreyfdde iadqpglvfd dnsraggetq samasqavte nshtlvessp ktdyvnellp
      601 slgtghgkna tnsphhgkri sragsvdtls pcesiasddl ildyeqsdas syeeqqqrve
      661 sntalqdmdd atilseleaq geevmrqwts llgtcqmlqq tnnsntvnnn vnggstnnnn
      721 qpaneignec dkttkvwrnr svtdtprsvd nlrnrqfssp lrtsrnvhsp sldsgdegsl
      781 rvdrntyqhm fqdivsiktm llklkrvlqe seengltrte tlnpfdnsmk nglfynlneg
      841 ntadvstspg sggnsiadel adlrrqvvfl qgqvedrdrt iqvlqfqisk lqgpngndgq
      901 tcalasnhnn vnpdtcnaat qtektrpvsa gpslvqtppq ddamwplisf rigkvesme
//
LOCUS       XP_034186818             828 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 16/45 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186818
VERSION     XP_034186818.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330927.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186818.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="ubiquitin specific protease 16/45 isoform X1"
                     /calculated_mol_wt=92445
     Region          71..125
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          <131..826
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <615..825
                     /region_name="Peptidase_C19K"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02667"
                     /db_xref="CDD:239132"
     CDS             1..828
                     /gene="Usp16-45"
                     /coded_by="XM_034330927.2:213..2699"
                     /db_xref="GeneID:117607339"
ORIGIN      
        1 mskkwnrhsd sngelsdgsi hscdvlritd cncssigkav nfnemkkcgm nikcttgcke
       61 ctsdskkpke eecamssrml iclecahqgc gkkesehale hykklhcdsc hsiagfgssf
      121 wcfdcaveiq nettnvdkke qkqilynlpk vgglmnlgnt cffnavlqcl aqtpflvkvl
      181 edlrlpgqkf vlpggkhkpa nsseevelpp iegtlegwgs ftsilcktli emqnsdgrqa
      241 yrpseilnsf kkktmqcmdg gqhdshellr hllelvrned lrryqsiilk evglnektnp
      301 ervenslksr vkfygnqasa rllgpelvfr gvlvstlecl dchhtsqsme pfldlsipvm
      361 adkpqppvlk rknsgfddtf nvmgnnisht pskyqlkkek kpvrknrknr kqenrnhdnd
      421 dnfisdknni aeenngsire sekgdadved nveadhfstk mlpgpspmnt cvtnspqgkt
      481 ldgldssesv enesgiqtgs sfstslvmns dltsptvpti slssvtskes pnspvsgvtn
      541 genlenikrp lsrlnliepv gpieqeeeee aiwddwkarr kteeyssevs ngingitsei
      601 skigiscnlq qsptryptke geysiqscln qftalelmsg snkvgceact arekkvkenc
      661 kmvctpstkq ylisrvpavl ifhlkrfqaq rtefrkvsrh vsfptlldla picknhkkpr
      721 vyalygvveh sgtirgghyv ayvksrlplk pddprwsflp pkdpqdtqen scssssdset
      781 eeatatlssa vkpppgkwyl isdsrvsevd ektvlqsqay llfyerif
//
LOCUS       XP_034186816             828 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 16/45 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186816
VERSION     XP_034186816.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330925.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186816.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="ubiquitin specific protease 16/45 isoform X1"
                     /calculated_mol_wt=92445
     Region          71..125
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          <131..826
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <615..825
                     /region_name="Peptidase_C19K"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02667"
                     /db_xref="CDD:239132"
     CDS             1..828
                     /gene="Usp16-45"
                     /coded_by="XM_034330925.2:234..2720"
                     /db_xref="GeneID:117607339"
ORIGIN      
        1 mskkwnrhsd sngelsdgsi hscdvlritd cncssigkav nfnemkkcgm nikcttgcke
       61 ctsdskkpke eecamssrml iclecahqgc gkkesehale hykklhcdsc hsiagfgssf
      121 wcfdcaveiq nettnvdkke qkqilynlpk vgglmnlgnt cffnavlqcl aqtpflvkvl
      181 edlrlpgqkf vlpggkhkpa nsseevelpp iegtlegwgs ftsilcktli emqnsdgrqa
      241 yrpseilnsf kkktmqcmdg gqhdshellr hllelvrned lrryqsiilk evglnektnp
      301 ervenslksr vkfygnqasa rllgpelvfr gvlvstlecl dchhtsqsme pfldlsipvm
      361 adkpqppvlk rknsgfddtf nvmgnnisht pskyqlkkek kpvrknrknr kqenrnhdnd
      421 dnfisdknni aeenngsire sekgdadved nveadhfstk mlpgpspmnt cvtnspqgkt
      481 ldgldssesv enesgiqtgs sfstslvmns dltsptvpti slssvtskes pnspvsgvtn
      541 genlenikrp lsrlnliepv gpieqeeeee aiwddwkarr kteeyssevs ngingitsei
      601 skigiscnlq qsptryptke geysiqscln qftalelmsg snkvgceact arekkvkenc
      661 kmvctpstkq ylisrvpavl ifhlkrfqaq rtefrkvsrh vsfptlldla picknhkkpr
      721 vyalygvveh sgtirgghyv ayvksrlplk pddprwsflp pkdpqdtqen scssssdset
      781 eeatatlssa vkpppgkwyl isdsrvsevd ektvlqsqay llfyerif
//
LOCUS       XP_034186819             828 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 16/45 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186819
VERSION     XP_034186819.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330928.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186819.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="ubiquitin specific protease 16/45 isoform X1"
                     /calculated_mol_wt=92445
     Region          71..125
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          <131..826
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <615..825
                     /region_name="Peptidase_C19K"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02667"
                     /db_xref="CDD:239132"
     CDS             1..828
                     /gene="Usp16-45"
                     /coded_by="XM_034330928.2:232..2718"
                     /db_xref="GeneID:117607339"
ORIGIN      
        1 mskkwnrhsd sngelsdgsi hscdvlritd cncssigkav nfnemkkcgm nikcttgcke
       61 ctsdskkpke eecamssrml iclecahqgc gkkesehale hykklhcdsc hsiagfgssf
      121 wcfdcaveiq nettnvdkke qkqilynlpk vgglmnlgnt cffnavlqcl aqtpflvkvl
      181 edlrlpgqkf vlpggkhkpa nsseevelpp iegtlegwgs ftsilcktli emqnsdgrqa
      241 yrpseilnsf kkktmqcmdg gqhdshellr hllelvrned lrryqsiilk evglnektnp
      301 ervenslksr vkfygnqasa rllgpelvfr gvlvstlecl dchhtsqsme pfldlsipvm
      361 adkpqppvlk rknsgfddtf nvmgnnisht pskyqlkkek kpvrknrknr kqenrnhdnd
      421 dnfisdknni aeenngsire sekgdadved nveadhfstk mlpgpspmnt cvtnspqgkt
      481 ldgldssesv enesgiqtgs sfstslvmns dltsptvpti slssvtskes pnspvsgvtn
      541 genlenikrp lsrlnliepv gpieqeeeee aiwddwkarr kteeyssevs ngingitsei
      601 skigiscnlq qsptryptke geysiqscln qftalelmsg snkvgceact arekkvkenc
      661 kmvctpstkq ylisrvpavl ifhlkrfqaq rtefrkvsrh vsfptlldla picknhkkpr
      721 vyalygvveh sgtirgghyv ayvksrlplk pddprwsflp pkdpqdtqen scssssdset
      781 eeatatlssa vkpppgkwyl isdsrvsevd ektvlqsqay llfyerif
//
LOCUS       XP_034186820             828 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 16/45 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186820
VERSION     XP_034186820.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330929.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186820.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="ubiquitin specific protease 16/45 isoform X1"
                     /calculated_mol_wt=92445
     Region          71..125
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          <131..826
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <615..825
                     /region_name="Peptidase_C19K"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02667"
                     /db_xref="CDD:239132"
     CDS             1..828
                     /gene="Usp16-45"
                     /coded_by="XM_034330929.2:226..2712"
                     /db_xref="GeneID:117607339"
ORIGIN      
        1 mskkwnrhsd sngelsdgsi hscdvlritd cncssigkav nfnemkkcgm nikcttgcke
       61 ctsdskkpke eecamssrml iclecahqgc gkkesehale hykklhcdsc hsiagfgssf
      121 wcfdcaveiq nettnvdkke qkqilynlpk vgglmnlgnt cffnavlqcl aqtpflvkvl
      181 edlrlpgqkf vlpggkhkpa nsseevelpp iegtlegwgs ftsilcktli emqnsdgrqa
      241 yrpseilnsf kkktmqcmdg gqhdshellr hllelvrned lrryqsiilk evglnektnp
      301 ervenslksr vkfygnqasa rllgpelvfr gvlvstlecl dchhtsqsme pfldlsipvm
      361 adkpqppvlk rknsgfddtf nvmgnnisht pskyqlkkek kpvrknrknr kqenrnhdnd
      421 dnfisdknni aeenngsire sekgdadved nveadhfstk mlpgpspmnt cvtnspqgkt
      481 ldgldssesv enesgiqtgs sfstslvmns dltsptvpti slssvtskes pnspvsgvtn
      541 genlenikrp lsrlnliepv gpieqeeeee aiwddwkarr kteeyssevs ngingitsei
      601 skigiscnlq qsptryptke geysiqscln qftalelmsg snkvgceact arekkvkenc
      661 kmvctpstkq ylisrvpavl ifhlkrfqaq rtefrkvsrh vsfptlldla picknhkkpr
      721 vyalygvveh sgtirgghyv ayvksrlplk pddprwsflp pkdpqdtqen scssssdset
      781 eeatatlssa vkpppgkwyl isdsrvsevd ektvlqsqay llfyerif
//
LOCUS       XP_034186821             828 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 16/45 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186821
VERSION     XP_034186821.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330930.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186821.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..828
                     /product="ubiquitin specific protease 16/45 isoform X1"
                     /calculated_mol_wt=92445
     Region          71..125
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          <131..826
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <615..825
                     /region_name="Peptidase_C19K"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02667"
                     /db_xref="CDD:239132"
     CDS             1..828
                     /gene="Usp16-45"
                     /coded_by="XM_034330930.2:204..2690"
                     /db_xref="GeneID:117607339"
ORIGIN      
        1 mskkwnrhsd sngelsdgsi hscdvlritd cncssigkav nfnemkkcgm nikcttgcke
       61 ctsdskkpke eecamssrml iclecahqgc gkkesehale hykklhcdsc hsiagfgssf
      121 wcfdcaveiq nettnvdkke qkqilynlpk vgglmnlgnt cffnavlqcl aqtpflvkvl
      181 edlrlpgqkf vlpggkhkpa nsseevelpp iegtlegwgs ftsilcktli emqnsdgrqa
      241 yrpseilnsf kkktmqcmdg gqhdshellr hllelvrned lrryqsiilk evglnektnp
      301 ervenslksr vkfygnqasa rllgpelvfr gvlvstlecl dchhtsqsme pfldlsipvm
      361 adkpqppvlk rknsgfddtf nvmgnnisht pskyqlkkek kpvrknrknr kqenrnhdnd
      421 dnfisdknni aeenngsire sekgdadved nveadhfstk mlpgpspmnt cvtnspqgkt
      481 ldgldssesv enesgiqtgs sfstslvmns dltsptvpti slssvtskes pnspvsgvtn
      541 genlenikrp lsrlnliepv gpieqeeeee aiwddwkarr kteeyssevs ngingitsei
      601 skigiscnlq qsptryptke geysiqscln qftalelmsg snkvgceact arekkvkenc
      661 kmvctpstkq ylisrvpavl ifhlkrfqaq rtefrkvsrh vsfptlldla picknhkkpr
      721 vyalygvveh sgtirgghyv ayvksrlplk pddprwsflp pkdpqdtqen scssssdset
      781 eeatatlssa vkpppgkwyl isdsrvsevd ektvlqsqay llfyerif
//
LOCUS       XP_034186822             827 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin specific protease 16/45 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186822
VERSION     XP_034186822.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330931.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186822.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..827
                     /product="ubiquitin specific protease 16/45 isoform X2"
                     /calculated_mol_wt=92289
     Region          71..125
                     /region_name="zf-UBP"
                     /note="Zn-finger in ubiquitin-hydrolases and other
                     protein; pfam02148"
                     /db_xref="CDD:460464"
     Region          <131..825
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <614..824
                     /region_name="Peptidase_C19K"
                     /note="A subfamily of Peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02667"
                     /db_xref="CDD:239132"
     CDS             1..827
                     /gene="Usp16-45"
                     /coded_by="XM_034330931.2:313..2796"
                     /db_xref="GeneID:117607339"
ORIGIN      
        1 mskkwnrhsd sngelsdgsi hscdvlritd cncssigkav nfnemkkcgm nikcttgcke
       61 ctsdskkpke eecamssrml iclecahqgc gkkesehale hykklhcdsc hsiagfgssf
      121 wcfdcaveiq nettnvdkke qkqilynlpk vgglmnlgnt cffnavlqcl aqtpflvkvl
      181 edlrlpgqkf vlpggkhkpa nsseevelpp iegtlegwgs ftsilcktli emqnsdgrqa
      241 yrpseilnsf kkktmqcmdg gqhdshellr hllelvrned lrryqsiilk evglnektnp
      301 ervenslksr vkfygnqasa rllgpelvfr gvlvstlecl dchhtsqsme pfldlsipvm
      361 adkpqppvlk knsgfddtfn vmgnnishtp skyqlkkekk pvrknrknrk qenrnhdndd
      421 nfisdknnia eenngsires ekgdadvedn veadhfstkm lpgpspmntc vtnspqgktl
      481 dgldssesve nesgiqtgss fstslvmnsd ltsptvptis lssvtskesp nspvsgvtng
      541 enlenikrpl srlnliepvg pieqeeeeea iwddwkarrk teeyssevsn gingitseis
      601 kigiscnlqq sptryptkeg eysiqsclnq ftalelmsgs nkvgceacta rekkvkenck
      661 mvctpstkqy lisrvpavli fhlkrfqaqr tefrkvsrhv sfptlldlap icknhkkprv
      721 yalygvvehs gtirgghyva yvksrlplkp ddprwsflpp kdpqdtqens cssssdsete
      781 eatatlssav kpppgkwyli sdsrvsevde ktvlqsqayl lfyerif
//
LOCUS       XP_034186823             679 aa            linear   INV 03-SEP-2025
DEFINITION  zinc finger CCCH-type containing protein regnase 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186823
VERSION     XP_034186823.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330932.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..679
                     /product="zinc finger CCCH-type containing protein regnase
                     1"
                     /calculated_mol_wt=75820
     Region          117..158
                     /region_name="UBA_6"
                     /note="UBA-like domain; pfam18039"
                     /db_xref="CDD:407876"
     Region          195..325
                     /region_name="PIN_Zc3h12-like"
                     /note="PRORP-like PIN domain of ribonuclease Zc3h12a and
                     related proteins; cd18729"
                     /db_xref="CDD:350296"
     Region          361..379
                     /region_name="zf-CCCH_2"
                     /note="RNA-binding, Nab2-type zinc finger; pfam14608"
                     /db_xref="CDD:464217"
     CDS             1..679
                     /gene="Regnase-1"
                     /coded_by="XM_034330932.2:321..2360"
                     /db_xref="GeneID:117607340"
ORIGIN      
        1 micvhnsict dsrrsldakg ydrlrfvegd mtvagkkyvd ctileaesii daedssydsd
       61 yeaedpigst nvhsqvelqv ttsnhsdvsr ttsdtlaaef aeyvtvqgsp pnqspgytar
      121 vefalklgyt erlvqtalqk lgpdpeqnel laeliklgan csqrsidtpe esdtvlendl
      181 vtnessgcss rlrpvvidgs nvamshgnke ifsckgikic vnwfkarghk eitvfvpkwr
      241 keasridnpi adqeilgele rdrllvftps rliggkrmvc yddryilrla aeidgivvsn
      301 dnyrdlaqes pefrkvieer ilmytfvndr fmppddplgr sgptlenflr ispkksdpap
      361 pcpyakkcty gnkckfrhpe rgphpqksvt eklvehvqkh lqakdpspsl llptnlssnl
      421 sqhqplcktr savtasvqrl psviksksve nvaieqptns gvqgtqtipa tssqgyhssg
      481 rtagmnkpgl nkseveppnm hrklqrqltl npiydprlrr ssqyqqassa nspspqstva
      541 psraqhrplt rhssyelpyt vdmnwdhfeg rqghqhvtri asapesyqaw lpnnsnmpsp
      601 spsnqqlaas drqlnvsvhp lyagmpsdmi relldirsei lhhlvtvfpe ehvwtamlmh
      661 pfetdaieic vailkmypq
//
LOCUS       XP_034186824             539 aa            linear   INV 03-SEP-2025
DEFINITION  fatty-acid amide hydrolase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034186824
VERSION     XP_034186824.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330933.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186824.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..539
                     /product="fatty-acid amide hydrolase 2"
                     /calculated_mol_wt=60198
     Region          67..539
                     /region_name="GatA"
                     /note="Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit
                     or related amidase [Translation, ribosomal structure and
                     biogenesis]; COG0154"
                     /db_xref="CDD:439924"
     CDS             1..539
                     /gene="LOC117607341"
                     /coded_by="XM_034330933.2:565..2184"
                     /db_xref="GeneID:117607341"
ORIGIN      
        1 mctsvknkky qnndtchsvf etfmnflryf ikcliiqfhr vfdclidfvf gfyynnkskk
       61 vppikndlll msaselaeki rikkvssvev vtafierake vnsiinavve drysealeea
      121 kqvdkllqes entdalkkek pflgvpfttk esneakgmlh tmglvsrrdy rseedatavl
      181 sikkagaili aktnvpelnl wtesrntlyg qtcnpydttr nvggssggea aiiascgsaf
      241 siasdiggst rmpaffngvf gykptsglis lkgiglrqsd fpnsmaqagp ickkaedlap
      301 ilkvlvgeka sllkldtpvn mktlnifyqk ssgdiraskv nsemrvallk avhhlkeltg
      361 satkvkipgs eysyrlwryw mteegvnfkl nitnkkyctn atteimkllt gkseytfavi
      421 mklidedffp kenaewakni tenmkeylld klkdngvlly psapfpasyh ytayfrpfnf
      481 gywclfnilk fpvcqvplgl skdglpigiq vvaapyndhi ciavaqelek afggwvppa
//
LOCUS       XP_034186833             481 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607344 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186833
VERSION     XP_034186833.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330942.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186833.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="uncharacterized protein LOC117607344 isoform X1"
                     /calculated_mol_wt=53929
     CDS             1..481
                     /gene="LOC117607344"
                     /coded_by="XM_034330942.2:409..1854"
                     /db_xref="GeneID:117607344"
ORIGIN      
        1 mqglcplclk nglkkkikll qinlqeaiwm ceeekcvwpf gcedftfcqr lvgkiwscyw
       61 ddnippklke nlilpvkpsi ipikdppkec issildlsps dnnlnisvke esnivlqint
      121 eekynlnnki itpslndnnl qsssnkikte rtenslnkhi kkeidvften iksinnvkgi
      181 pkvtsiekti idisnvkten qisvnqdqnk nilsnnvntn tvehnlslpl ndkiqtipds
      241 sdykelpklg psknvqllne vtakpnlnvt tmqldglppi tlafempvst tvpeivtsnv
      301 rqndckrivt ecltkpitld mesvsskrnv tsgkqyekfs fsalkkkkei nnstnassns
      361 nsfqnvkpkl etknvannsi anvkkrscte nfhdiqsias ynknfkpits vtdvtdtskl
      421 nqvnnyessl pnqdvsnnee tisdtststa inidtvltdf ltndynvsed indewlnsll
      481 s
//
LOCUS       XP_076548182             452 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607344 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548182
VERSION     XP_076548182.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692067.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="uncharacterized protein LOC117607344 isoform X2"
                     /calculated_mol_wt=50609
     CDS             1..452
                     /gene="LOC117607344"
                     /coded_by="XM_076692067.1:387..1745"
                     /db_xref="GeneID:117607344"
ORIGIN      
        1 mceeekcvwp fgcedftfcq rlvgkiwscy wddnippklk enlilpvkps iipikdppke
       61 cissildlsp sdnnlnisvk eesnivlqin teekynlnnk iitpslndnn lqsssnkikt
      121 ertenslnkh ikkeidvfte niksinnvkg ipkvtsiekt iidisnvkte nqisvnqdqn
      181 knilsnnvnt ntvehnlslp lndkiqtipd ssdykelpkl gpsknvqlln evtakpnlnv
      241 ttmqldglpp itlafempvs ttvpeivtsn vrqndckriv tecltkpitl dmesvsskrn
      301 vtsgkqyekf sfsalkkkke innstnassn snsfqnvkpk letknvanns ianvkkrsct
      361 enfhdiqsia synknfkpit svtdvtdtsk lnqvnnyess lpnqdvsnne etisdtstst
      421 ainidtvltd fltndynvse dindewlnsl ls
//
LOCUS       XP_034186834             452 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607344 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186834
VERSION     XP_034186834.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330943.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186834.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..452
                     /product="uncharacterized protein LOC117607344 isoform X2"
                     /calculated_mol_wt=50609
     CDS             1..452
                     /gene="LOC117607344"
                     /coded_by="XM_034330943.2:85..1443"
                     /db_xref="GeneID:117607344"
ORIGIN      
        1 mceeekcvwp fgcedftfcq rlvgkiwscy wddnippklk enlilpvkps iipikdppke
       61 cissildlsp sdnnlnisvk eesnivlqin teekynlnnk iitpslndnn lqsssnkikt
      121 ertenslnkh ikkeidvfte niksinnvkg ipkvtsiekt iidisnvkte nqisvnqdqn
      181 knilsnnvnt ntvehnlslp lndkiqtipd ssdykelpkl gpsknvqlln evtakpnlnv
      241 ttmqldglpp itlafempvs ttvpeivtsn vrqndckriv tecltkpitl dmesvsskrn
      301 vtsgkqyekf sfsalkkkke innstnassn snsfqnvkpk letknvanns ianvkkrsct
      361 enfhdiqsia synknfkpit svtdvtdtsk lnqvnnyess lpnqdvsnne etisdtstst
      421 ainidtvltd fltndynvse dindewlnsl ls
//
LOCUS       XP_034186835             272 aa            linear   INV 03-SEP-2025
DEFINITION  PSME3-interacting protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186835
VERSION     XP_034186835.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330944.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="PSME3-interacting protein isoform X1"
                     /calculated_mol_wt=30973
     Region          5..106
                     /region_name="FAM192A_Fyv6_N"
                     /note="FAM192A/Fyv6, N-terminal domain; pfam10187"
                     /db_xref="CDD:462987"
     CDS             1..272
                     /gene="LOC117607345"
                     /coded_by="XM_034330944.2:589..1407"
                     /db_xref="GeneID:117607345"
ORIGIN      
        1 mssgfiseae iaeqrrirqe ewervrtadq pleapeeqyd prslyerlqe qknkrdseye
       61 eahklknmik gldddevefl dlvdrtklee erkknleeek emrdfkaava slqekslnek
      121 lkqelknpqi vnknvssgss rtsqlkllag vvvkrsekqk qgditrgvkr klspveennt
      181 pkkekcevne qpvseslkcd sdtfedvsfq nknvvslgsg mkcigilpgl gsyedssdsd
      241 cssdtdqdpe pnhskydllg rkievskdke ts
//
LOCUS       XP_034186836             272 aa            linear   INV 03-SEP-2025
DEFINITION  PSME3-interacting protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186836
VERSION     XP_034186836.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330945.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="PSME3-interacting protein isoform X1"
                     /calculated_mol_wt=30973
     Region          5..106
                     /region_name="FAM192A_Fyv6_N"
                     /note="FAM192A/Fyv6, N-terminal domain; pfam10187"
                     /db_xref="CDD:462987"
     CDS             1..272
                     /gene="LOC117607345"
                     /coded_by="XM_034330945.2:255..1073"
                     /db_xref="GeneID:117607345"
ORIGIN      
        1 mssgfiseae iaeqrrirqe ewervrtadq pleapeeqyd prslyerlqe qknkrdseye
       61 eahklknmik gldddevefl dlvdrtklee erkknleeek emrdfkaava slqekslnek
      121 lkqelknpqi vnknvssgss rtsqlkllag vvvkrsekqk qgditrgvkr klspveennt
      181 pkkekcevne qpvseslkcd sdtfedvsfq nknvvslgsg mkcigilpgl gsyedssdsd
      241 cssdtdqdpe pnhskydllg rkievskdke ts
//
LOCUS       XP_034186837             272 aa            linear   INV 03-SEP-2025
DEFINITION  PSME3-interacting protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186837
VERSION     XP_034186837.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330946.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="PSME3-interacting protein isoform X1"
                     /calculated_mol_wt=30973
     Region          5..106
                     /region_name="FAM192A_Fyv6_N"
                     /note="FAM192A/Fyv6, N-terminal domain; pfam10187"
                     /db_xref="CDD:462987"
     CDS             1..272
                     /gene="LOC117607345"
                     /coded_by="XM_034330946.2:446..1264"
                     /db_xref="GeneID:117607345"
ORIGIN      
        1 mssgfiseae iaeqrrirqe ewervrtadq pleapeeqyd prslyerlqe qknkrdseye
       61 eahklknmik gldddevefl dlvdrtklee erkknleeek emrdfkaava slqekslnek
      121 lkqelknpqi vnknvssgss rtsqlkllag vvvkrsekqk qgditrgvkr klspveennt
      181 pkkekcevne qpvseslkcd sdtfedvsfq nknvvslgsg mkcigilpgl gsyedssdsd
      241 cssdtdqdpe pnhskydllg rkievskdke ts
//
LOCUS       XP_034186838             271 aa            linear   INV 03-SEP-2025
DEFINITION  PSME3-interacting protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186838
VERSION     XP_034186838.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330947.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..271
                     /product="PSME3-interacting protein isoform X2"
                     /calculated_mol_wt=30886
     Region          5..106
                     /region_name="FAM192A_Fyv6_N"
                     /note="FAM192A/Fyv6, N-terminal domain; pfam10187"
                     /db_xref="CDD:462987"
     CDS             1..271
                     /gene="LOC117607345"
                     /coded_by="XM_034330947.2:589..1404"
                     /db_xref="GeneID:117607345"
ORIGIN      
        1 mssgfiseae iaeqrrirqe ewervrtadq pleapeeqyd prslyerlqe qknkrdseye
       61 eahklknmik gldddevefl dlvdrtklee erkknleeek emrdfkaava slqekslnek
      121 lkqelknpqi vnknvssgsr tsqlkllagv vvkrsekqkq gditrgvkrk lspveenntp
      181 kkekcevneq pvseslkcds dtfedvsfqn knvvslgsgm kcigilpglg syedssdsdc
      241 ssdtdqdpep nhskydllgr kievskdket s
//
LOCUS       XP_034186839             209 aa            linear   INV 03-SEP-2025
DEFINITION  peptidyl-prolyl cis-trans isomerase [Osmia lignaria lignaria].
ACCESSION   XP_034186839
VERSION     XP_034186839.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330948.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /calculated_mol_wt=22616
     Region          49..207
                     /region_name="cyclophilin_ABH_like"
                     /note="Cyclophilin A, B and H-like cyclophilin-type
                     peptidylprolyl cis- trans isomerase (PPIase) domain. This
                     family represents the archetypal cystolic cyclophilin
                     similar to human cyclophilins A, B and H. PPIase is an
                     enzyme which accelerates protein folding...; cd01926"
                     /db_xref="CDD:238907"
     Site            order(99..100,105,156,158,166)
                     /site_type="active"
                     /db_xref="CDD:238907"
     CDS             1..209
                     /gene="LOC117607346"
                     /coded_by="XM_034330948.2:150..779"
                     /db_xref="GeneID:117607346"
ORIGIN      
        1 malrsicrls idstfshlnh lgatarprrp sftlvsrtfa avaekmglpr vffdmtadtk
       61 pvgrivmelr ndvvpktaen fralctgekg ygykgssfhr vipnfmcqgg dftnhngtgg
      121 esiygskfdd enfvlkhtep gilsmanagp ntngsqffit saktswldgk hvvfgkvveg
      181 mdvvrklegm gsqsgktskk ivvadcgel
//
LOCUS       XP_034186840             207 aa            linear   INV 03-SEP-2025
DEFINITION  ribosomal protein S7 [Osmia lignaria lignaria].
ACCESSION   XP_034186840
VERSION     XP_034186840.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330949.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..207
                     /product="ribosomal protein S7"
                     /calculated_mol_wt=23583
     Region          21..202
                     /region_name="Ribosomal_S7e"
                     /note="Ribosomal protein S7e; pfam01251"
                     /db_xref="CDD:460135"
     CDS             1..207
                     /gene="RpS7"
                     /coded_by="XM_034330949.2:57..680"
                     /db_xref="GeneID:117607347"
ORIGIN      
        1 mvagsfshgm dvgkmftana kiiksggaep dafeasisqa llelemnsdl ksqlrelyit
       61 kareietnnk kciiiyvpmp klkafqkiqt rlvrelekkf sgkhvmfvge rkilpkptrk
      121 trtknkqkrp rsrtltavyd alledlvypv eivgkririk ldgtqlikvh ldkneqtnie
      181 hkvdtfaavy kkltgrdvtf efpetyv
//
LOCUS       XP_034186842             169 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607348 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186842
VERSION     XP_034186842.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330951.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..169
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..169
                     /product="uncharacterized protein LOC117607348 isoform X1"
                     /calculated_mol_wt=18840
     Region          44..136
                     /region_name="TFP"
                     /note="three-fingered protein (TFP) fold found in Ly6/uPAR
                     (LU) and snake toxin superfamily; cd00117"
                     /db_xref="CDD:467060"
     CDS             1..169
                     /gene="LOC117607348"
                     /coded_by="XM_034330951.2:1..510"
                     /db_xref="GeneID:117607348"
ORIGIN      
        1 mselkntkef vflnfiekis mqlsgqllst ehrthaaaer vndlwcyqcd tmedgercsn
       61 ltgnytafeh kctgdkrtcm vkrfsyttst edstsspqtw smernctskc epgciviger
      121 tklsacttcc eqsfcnvgtg asndltmkgi dlflalilqi tltiimyps
//
LOCUS       XP_034186843             167 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607348 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186843
VERSION     XP_034186843.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330952.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..167
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..167
                     /product="uncharacterized protein LOC117607348 isoform X2"
                     /calculated_mol_wt=18268
     Region          42..134
                     /region_name="TFP"
                     /note="three-fingered protein (TFP) fold found in Ly6/uPAR
                     (LU) and snake toxin superfamily; cd00117"
                     /db_xref="CDD:467060"
     CDS             1..167
                     /gene="LOC117607348"
                     /coded_by="XM_034330952.2:74..577"
                     /db_xref="GeneID:117607348"
ORIGIN      
        1 mtgdtcaivt cflflasitl angqllsteh rthaaaervn dlwcyqcdtm edgercsnlt
       61 gnytafehkc tgdkrtcmvk rfsyttsted stsspqtwsm ernctskcep gcivigertk
      121 lsacttcceq sfcnvgtgas ndltmkgidl flalilqitl tiimyps
//
LOCUS       XP_034186846             105 aa            linear   INV 03-SEP-2025
DEFINITION  defensin 2 [Osmia lignaria lignaria].
ACCESSION   XP_034186846
VERSION     XP_034186846.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330955.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186846.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="defensin 2"
                     /calculated_mol_wt=11305
     Region          65..105
                     /region_name="DEFL"
                     /note="defensin-like domain family; cl41745"
                     /db_xref="CDD:425376"
     CDS             1..105
                     /gene="Def2"
                     /coded_by="XM_034330955.2:85..402"
                     /db_xref="GeneID:117607351"
ORIGIN      
        1 mkltvvcila tiacvygasv peavydgpvy elkpiedgsg gveprqvess destdltplr
       61 qrrvtcdvls wqsqwftvnh sacaikclaq rrrggscrgg vcvcr
//
LOCUS       XP_034190915             311 aa            linear   INV 03-SEP-2025
DEFINITION  ntrilase and fragile histidine triad fusion protein NitFhit isoform
            X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190915
VERSION     XP_034190915.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335024.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190915.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="ntrilase and fragile histidine triad fusion
                     protein NitFhit isoform X1"
                     /calculated_mol_wt=34962
     Region          31..297
                     /region_name="nit"
                     /note="Nit1, Nit 2, and related proteins, and the
                     Nit1-like domain of NitFhit (class 10 nitrilases);
                     cd07572"
                     /db_xref="CDD:143596"
     Site            order(69,125,142,146,159,185..186,188..189,210)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:143596"
     Site            order(69,142,185)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:143596"
     Site            order(143..144,165..167,186,189..193,195..196,218..219,
                     222..223,225..227,229..230,260,290..291,294..295,297)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143596"
     CDS             1..311
                     /gene="NitFhit"
                     /coded_by="XM_034335024.2:352..1287"
                     /db_xref="GeneID:117609117"
ORIGIN      
        1 mismrfirnf nsiiycrkqf drhfhimtnp liavcqmtst ndkeknlqtv sevvskakar
       61 sacitffpea cdyladnkkd vvamaehlng ptvsnykeia kknnmwlsfg glhealtene
      121 kcignthivi sskgdivgvy rkmhlfdmdn kntgvrlles dyvlpgqkie ppistpvgkl
      181 alsicydmrf pelslslrnm gaeiltypsa ftyqtgaahw evllraraie tqcyvvaaaq
      241 tgthnkkrvs wghamvvdpw gtvvaqcsdk tgialaeidl nfikqvrenm pcenhrrtdl
      301 ypkmepikpv v
//
LOCUS       XP_034190916             275 aa            linear   INV 03-SEP-2025
DEFINITION  ntrilase and fragile histidine triad fusion protein NitFhit isoform
            X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190916
VERSION     XP_034190916.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335025.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190916.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..275
                     /product="ntrilase and fragile histidine triad fusion
                     protein NitFhit isoform X2"
                     /calculated_mol_wt=30534
     Region          1..261
                     /region_name="nit"
                     /note="Nit1, Nit 2, and related proteins, and the
                     Nit1-like domain of NitFhit (class 10 nitrilases);
                     cd07572"
                     /db_xref="CDD:143596"
     Site            order(33,89,106,110,123,149..150,152..153,174)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:143596"
     Site            order(33,106,149)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:143596"
     Site            order(107..108,129..131,150,153..157,159..160,182..183,
                     186..187,189..191,193..194,224,254..255,258..259,261)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143596"
     CDS             1..275
                     /gene="NitFhit"
                     /coded_by="XM_034335025.2:409..1236"
                     /db_xref="GeneID:117609117"
ORIGIN      
        1 mtstndkekn lqtvsevvsk akarsacitf fpeacdylad nkkdvvamae hlngptvsny
       61 keiakknnmw lsfgglheal tenekcignt hivisskgdi vgvyrkmhlf dmdnkntgvr
      121 llesdyvlpg qkieppistp vgklalsicy dmrfpelsls lrnmgaeilt ypsaftyqtg
      181 aahwevllra raietqcyvv aaaqtgthnk krvswghamv vdpwgtvvaq csdktgiala
      241 eidlnfikqv renmpcenhr rtdlypkmep ikpvv
//
LOCUS       XP_034190766             201 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609039 [Osmia lignaria lignaria].
ACCESSION   XP_034190766
VERSION     XP_034190766.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334875.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..201
                     /product="uncharacterized protein LOC117609039"
                     /calculated_mol_wt=22475
     CDS             1..201
                     /gene="LOC117609039"
                     /coded_by="XM_034334875.2:317..922"
                     /db_xref="GeneID:117609039"
ORIGIN      
        1 mdehpekvtv aekvegrvdp rgarkrssrk srgrrkrgee flectkgvpt leectknive
       61 ggnrrclsnl dlqdvtgcic seiaanfrlh igkkseatgc srspspemth tiriamkqhd
      121 rsacgvsdde etegnrrsrp etnrknrqrt gkevdreecs ngvqdtlnft lncsglrlac
      181 rdtnlsteia sekqqkskir n
//
LOCUS       XP_034183037             831 aa            linear   INV 03-SEP-2025
DEFINITION  guanylyl cyclase at 88E [Osmia lignaria lignaria].
ACCESSION   XP_034183037
VERSION     XP_034183037.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327146.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="guanylyl cyclase at 88E"
                     /calculated_mol_wt=94783
     Region          2..163
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          201..407
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          386..572
                     /region_name="CYCc"
                     /note="Adenylyl- / guanylyl cyclase, catalytic domain;
                     smart00044"
                     /db_xref="CDD:214485"
     Site            order(427,429..434,469,471..473,476,539..541,545..546,
                     549..550,585)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(429,473)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(434,446,449..450,453,457,469..470,527,530,540..543,
                     546,585)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..831
                     /gene="Gyc88E"
                     /coded_by="XM_034327146.2:923..3418"
                     /db_xref="GeneID:117605617"
ORIGIN      
        1 myglilenls eyirqvyged rweeirrqaa vdqpsfsvhq vypenliprl akkaiqvlgi
       61 tekeffdqmg vhfvgfvgqy gydrvlsvlg rhvrdflngl dnlheylkfs yprmrapsfi
      121 cenetrqglt lhyrskrrgf vyytmgqire varhfyhkel qielvreevl fdtvhvtfql
      181 tfdnraftqa sltmtreekh lpigasvlfe ifpfcivfgs dmivrsigns lmvilpdlvg
      241 kkithffdlv rpliafkfhs ilnrtnnife lvtvepilte rpsdrrgnei llsdeldsid
      301 drtlrlkgqm iymdnwkmmm ylgtpvmpdl naliatglyi ndlsmhdfsr dlmlagtqqs
      361 velklaldqe qlkskklees mrkldeemkr tdellyqmip kqvadrlrng espidtcemf
      421 dsvsilfsdv vtfteicsri tpmevvsmln amyslfdtlt ernrvykvet igdaymvvsg
      481 apvkendhad rvcdmaldmv daitnlkdrs tglhlqirvg ihsgavvagi vglkmprycl
      541 fgdsvntasr meatsqamqi hisqstrell spsykvkerg eievkgkgtm ktywlkkreh
      601 rpsltkgiii qeppqwhtrn tekrvsasts aasaaaiifd nqncldtssr rgskiasptp
      661 tdstfllete rrmyspitfq dvarrsvans ptknndsrec rsnsmgavvt rnsemfslls
      721 dteehfrqhr dslstrvenq taviqskpev ntnsssssde sreeracsvd ieapkreknd
      781 qcekskeyys ayqssdigns qsltqqdqcc pgftmsksgk trhqnngcsi a
//
LOCUS       XP_076548184             831 aa            linear   INV 03-SEP-2025
DEFINITION  guanylyl cyclase at 88E [Osmia lignaria lignaria].
ACCESSION   XP_076548184
VERSION     XP_076548184.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692069.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="guanylyl cyclase at 88E"
                     /calculated_mol_wt=94783
     Region          2..163
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          201..407
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          386..572
                     /region_name="CYCc"
                     /note="Adenylyl- / guanylyl cyclase, catalytic domain;
                     smart00044"
                     /db_xref="CDD:214485"
     Site            order(427,429..434,469,471..473,476,539..541,545..546,
                     549..550,585)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(429,473)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(434,446,449..450,453,457,469..470,527,530,540..543,
                     546,585)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..831
                     /gene="Gyc88E"
                     /coded_by="XM_076692069.1:700..3195"
                     /db_xref="GeneID:117605617"
ORIGIN      
        1 myglilenls eyirqvyged rweeirrqaa vdqpsfsvhq vypenliprl akkaiqvlgi
       61 tekeffdqmg vhfvgfvgqy gydrvlsvlg rhvrdflngl dnlheylkfs yprmrapsfi
      121 cenetrqglt lhyrskrrgf vyytmgqire varhfyhkel qielvreevl fdtvhvtfql
      181 tfdnraftqa sltmtreekh lpigasvlfe ifpfcivfgs dmivrsigns lmvilpdlvg
      241 kkithffdlv rpliafkfhs ilnrtnnife lvtvepilte rpsdrrgnei llsdeldsid
      301 drtlrlkgqm iymdnwkmmm ylgtpvmpdl naliatglyi ndlsmhdfsr dlmlagtqqs
      361 velklaldqe qlkskklees mrkldeemkr tdellyqmip kqvadrlrng espidtcemf
      421 dsvsilfsdv vtfteicsri tpmevvsmln amyslfdtlt ernrvykvet igdaymvvsg
      481 apvkendhad rvcdmaldmv daitnlkdrs tglhlqirvg ihsgavvagi vglkmprycl
      541 fgdsvntasr meatsqamqi hisqstrell spsykvkerg eievkgkgtm ktywlkkreh
      601 rpsltkgiii qeppqwhtrn tekrvsasts aasaaaiifd nqncldtssr rgskiasptp
      661 tdstfllete rrmyspitfq dvarrsvans ptknndsrec rsnsmgavvt rnsemfslls
      721 dteehfrqhr dslstrvenq taviqskpev ntnsssssde sreeracsvd ieapkreknd
      781 qcekskeyys ayqssdigns qsltqqdqcc pgftmsksgk trhqnngcsi a
//
LOCUS       XP_034183036             831 aa            linear   INV 03-SEP-2025
DEFINITION  guanylyl cyclase at 88E [Osmia lignaria lignaria].
ACCESSION   XP_034183036
VERSION     XP_034183036.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327145.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="guanylyl cyclase at 88E"
                     /calculated_mol_wt=94783
     Region          2..163
                     /region_name="HNOB"
                     /note="Haem-NO-binding; pfam07700"
                     /db_xref="CDD:462233"
     Region          201..407
                     /region_name="HNOBA"
                     /note="Heme NO binding associated; pfam07701"
                     /db_xref="CDD:462234"
     Region          386..572
                     /region_name="CYCc"
                     /note="Adenylyl- / guanylyl cyclase, catalytic domain;
                     smart00044"
                     /db_xref="CDD:214485"
     Site            order(427,429..434,469,471..473,476,539..541,545..546,
                     549..550,585)
                     /site_type="active"
                     /note="nucleotidyl binding site [active]"
                     /db_xref="CDD:143636"
     Site            order(429,473)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:143636"
     Site            order(434,446,449..450,453,457,469..470,527,530,540..543,
                     546,585)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:143636"
     CDS             1..831
                     /gene="Gyc88E"
                     /coded_by="XM_034327145.2:630..3125"
                     /db_xref="GeneID:117605617"
ORIGIN      
        1 myglilenls eyirqvyged rweeirrqaa vdqpsfsvhq vypenliprl akkaiqvlgi
       61 tekeffdqmg vhfvgfvgqy gydrvlsvlg rhvrdflngl dnlheylkfs yprmrapsfi
      121 cenetrqglt lhyrskrrgf vyytmgqire varhfyhkel qielvreevl fdtvhvtfql
      181 tfdnraftqa sltmtreekh lpigasvlfe ifpfcivfgs dmivrsigns lmvilpdlvg
      241 kkithffdlv rpliafkfhs ilnrtnnife lvtvepilte rpsdrrgnei llsdeldsid
      301 drtlrlkgqm iymdnwkmmm ylgtpvmpdl naliatglyi ndlsmhdfsr dlmlagtqqs
      361 velklaldqe qlkskklees mrkldeemkr tdellyqmip kqvadrlrng espidtcemf
      421 dsvsilfsdv vtfteicsri tpmevvsmln amyslfdtlt ernrvykvet igdaymvvsg
      481 apvkendhad rvcdmaldmv daitnlkdrs tglhlqirvg ihsgavvagi vglkmprycl
      541 fgdsvntasr meatsqamqi hisqstrell spsykvkerg eievkgkgtm ktywlkkreh
      601 rpsltkgiii qeppqwhtrn tekrvsasts aasaaaiifd nqncldtssr rgskiasptp
      661 tdstfllete rrmyspitfq dvarrsvans ptknndsrec rsnsmgavvt rnsemfslls
      721 dteehfrqhr dslstrvenq taviqskpev ntnsssssde sreeracsvd ieapkreknd
      781 qcekskeyys ayqssdigns qsltqqdqcc pgftmsksgk trhqnngcsi a
//
LOCUS       XP_034186744            2521 aa            linear   INV 03-SEP-2025
DEFINITION  nuak family kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186744
VERSION     XP_034186744.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330853.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186744.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2521
                     /product="nuak family kinase 1 isoform X1"
                     /calculated_mol_wt=279256
     Region          32..285
                     /region_name="STKc_NUAK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     novel (nua) kinase family NUAK; cd14073"
                     /db_xref="CDD:270975"
     Site            order(40..44,48,61,63,95,111..114,118,120..121,123,
                     156..157,159,161..162,164,174..175,178,191..196,198,
                     228..231,233)
                     /site_type="active"
                     /db_xref="CDD:270975"
     Site            order(40..43,48,61,63,95,111..114,118,161..162,164,
                     174..175)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270975"
     Site            order(118,120..121,123,157,159,161,178,191..196,198,
                     228..231,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270975"
     Site            174..196
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270975"
     CDS             1..2521
                     /gene="Nuak1"
                     /coded_by="XM_034330853.2:268..7833"
                     /db_xref="GeneID:117607305"
ORIGIN      
        1 mvvgeasihn imggmestgg vrlhnhkrkl kqrfdiikkl gqgtygkvql ginketgqev
       61 aiktikkcki eteadlirir reiqimssvq hpniihiyev fenrekmvlv meyaaggely
      121 dylserkklt eqearrifrq iatavfychk hkichrdlkl enilldqvgn akiadfglsn
      181 vfdeqrllnt fcgsplyasp eivkgtpyhg pevdcwslgv llytlvygam pfdgsnfkrl
      241 vkqisqsdyy epkkpspasp likdmltvrr ckradiekic thwwvnegye qncldiaeel
      301 aaqtpvrldl llslvpqsas aeklvvgdqq tagdvpndms senlvptrch svgslmeldq
      361 nnsdrrirel leeeprtapi tgeakrklet tpsmdetaaa gakrkersrk kekpeerepr
      421 tyksasrhhs apipnaitee amevdptvtv pinpsvdldt vessaaclel ikecnerfps
      481 kersetpvda rneaekkssk pdeaqmvdea anatpaqptt pvsesvtvsd rndasasnda
      541 inleatqref kklkekalsl dselssetsd vpgktferrr skifetaekf nqmasnvdne
      601 kpkkifipgv nvggakrvfe rkaslssast ppptkpsask iiidvptdak knekvkqepe
      661 gqaeeekkne dakkravdii tgaigkppvq krangsppts ppsqepkklp lkipvgtndl
      721 rsatvsvstp vetkfpfedk esdrnkrati stsvsgsana essnvddsqd epkmsskmei
      781 tlksatlprp rkttkaeisl sstkpqeipv afkseveaki dafqpqklrt qrsevafpva
      841 aavpqanrss slepegrprs tapkeriipi qvetgnqqte hsstpppskl ppmpqrsisq
      901 rsgslsrqst adsdtdsalg stvgpepirk spreyiipia vegggyvtpr sgsvepeskt
      961 stptsanapr srfgrprrms sllsdasede spfstlhrds edllqrhmhr lrssrpsrqp
     1021 pehadslssg edddddgfel ltaenlfstl lsrvrsltqr lnvddnrttg fprsrlfdrl
     1081 gsnpsqafwg lnkplssyqt yvetrtvttr lsesqfrhsl sregdvfsrk dpvnssnpat
     1141 pktpnspgsh gtpsretvfd sgnvtlprdk verqdveaep arvrketqes leqsftpsla
     1201 rrlsrtlieq dtgtgsrspy etrcgsptge tsslldasea adrsvstvsd qteyrqgags
     1261 rsgvrransl lersierlgs rresprrsvs lfegngdrey gsedryaegd dfagfskrta
     1321 tgldkwykda agkrnanklr resfrsyrtd kiqeetgdgd klecnadngs isectsasvc
     1381 dsntnlldat tssvsaksye ssstesnpss nlldykssaa yksaelsrnf enrllaaenl
     1441 ikesklknlv ptgeaskfdr nlsssyrdtd kcekrslisi getlpatisk rrscipslrl
     1501 rsgsltreps ssvvdrrksf adslgvglaa rsvspeksll sklfkgsgnr eneakekeke
     1561 kektqkckqr risrflrpdf fdtpreesqy akekeaqkaa enerrksrfm rrktengavv
     1621 aressveqek qrekelknei nslkrdsrls rdaeesfevk kngdateess kngflhslek
     1681 kleklrsneq seqseqseek kmagsnatmn grvandnare hsappaesss kersstkrav
     1741 svedlsskkk tgqnssrsrv ssvlglfksg dakqparsqn ailsklkrsp pkatkadasl
     1801 aedtvsttst stsasriptk lakcdskatk kiadtkktdr gekmvssdkk sptkeaprai
     1861 sakekrasse keskekmvdr eveekdstag etvekrslmt kakkttrtsa esgsavdadk
     1921 kvkrlvkkpg ensegdsegt kkkkivrvak kivkkssdts edktmteeke ksgkirakkk
     1981 tvtrsemnsg esngsdsvas ngkakengie tldrnetaee trneqqksqe sqrpnrsnlk
     2041 ldlskipqhs frnstpkkds pksespkvsp kstgndpvpd klmeclskvt hrasitgnki
     2101 vidkplrakd vaelkrevte cakiieshaq sepvsqpvdr eivteevesk rtapeaitpa
     2161 vpindsmeka qtpkesitpi dymnelfspv depesfdsws isstelnhar pdlhsptsps
     2221 rflyaksdnp dsvierirrr sfysrfndkr rpsltapppg lslptsatlp rkfsfsghre
     2281 hrdrgskyag tglglmsnsr htgdrsysly gdegvalgrn rsldrsrysd tspyvsdlks
     2341 ptehlptlss sydplrrylr sptfdlsasr sryhsadfad pdlpayrtpp hlsslllndh
     2401 aidthatsss ssslgsstlp rkygsthaep ktiqyyeell apssvdylts arrsplssey
     2461 lnrcengyyn gnleaqstda krkpmmeftd qsteqirskt erarspsttt eagtvptddh
     2521 s
//
LOCUS       XP_034186745            2521 aa            linear   INV 03-SEP-2025
DEFINITION  nuak family kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186745
VERSION     XP_034186745.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330854.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186745.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2521
                     /product="nuak family kinase 1 isoform X1"
                     /calculated_mol_wt=279256
     Region          32..285
                     /region_name="STKc_NUAK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     novel (nua) kinase family NUAK; cd14073"
                     /db_xref="CDD:270975"
     Site            order(40..44,48,61,63,95,111..114,118,120..121,123,
                     156..157,159,161..162,164,174..175,178,191..196,198,
                     228..231,233)
                     /site_type="active"
                     /db_xref="CDD:270975"
     Site            order(40..43,48,61,63,95,111..114,118,161..162,164,
                     174..175)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270975"
     Site            order(118,120..121,123,157,159,161,178,191..196,198,
                     228..231,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270975"
     Site            174..196
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270975"
     CDS             1..2521
                     /gene="Nuak1"
                     /coded_by="XM_034330854.2:263..7828"
                     /db_xref="GeneID:117607305"
ORIGIN      
        1 mvvgeasihn imggmestgg vrlhnhkrkl kqrfdiikkl gqgtygkvql ginketgqev
       61 aiktikkcki eteadlirir reiqimssvq hpniihiyev fenrekmvlv meyaaggely
      121 dylserkklt eqearrifrq iatavfychk hkichrdlkl enilldqvgn akiadfglsn
      181 vfdeqrllnt fcgsplyasp eivkgtpyhg pevdcwslgv llytlvygam pfdgsnfkrl
      241 vkqisqsdyy epkkpspasp likdmltvrr ckradiekic thwwvnegye qncldiaeel
      301 aaqtpvrldl llslvpqsas aeklvvgdqq tagdvpndms senlvptrch svgslmeldq
      361 nnsdrrirel leeeprtapi tgeakrklet tpsmdetaaa gakrkersrk kekpeerepr
      421 tyksasrhhs apipnaitee amevdptvtv pinpsvdldt vessaaclel ikecnerfps
      481 kersetpvda rneaekkssk pdeaqmvdea anatpaqptt pvsesvtvsd rndasasnda
      541 inleatqref kklkekalsl dselssetsd vpgktferrr skifetaekf nqmasnvdne
      601 kpkkifipgv nvggakrvfe rkaslssast ppptkpsask iiidvptdak knekvkqepe
      661 gqaeeekkne dakkravdii tgaigkppvq krangsppts ppsqepkklp lkipvgtndl
      721 rsatvsvstp vetkfpfedk esdrnkrati stsvsgsana essnvddsqd epkmsskmei
      781 tlksatlprp rkttkaeisl sstkpqeipv afkseveaki dafqpqklrt qrsevafpva
      841 aavpqanrss slepegrprs tapkeriipi qvetgnqqte hsstpppskl ppmpqrsisq
      901 rsgslsrqst adsdtdsalg stvgpepirk spreyiipia vegggyvtpr sgsvepeskt
      961 stptsanapr srfgrprrms sllsdasede spfstlhrds edllqrhmhr lrssrpsrqp
     1021 pehadslssg edddddgfel ltaenlfstl lsrvrsltqr lnvddnrttg fprsrlfdrl
     1081 gsnpsqafwg lnkplssyqt yvetrtvttr lsesqfrhsl sregdvfsrk dpvnssnpat
     1141 pktpnspgsh gtpsretvfd sgnvtlprdk verqdveaep arvrketqes leqsftpsla
     1201 rrlsrtlieq dtgtgsrspy etrcgsptge tsslldasea adrsvstvsd qteyrqgags
     1261 rsgvrransl lersierlgs rresprrsvs lfegngdrey gsedryaegd dfagfskrta
     1321 tgldkwykda agkrnanklr resfrsyrtd kiqeetgdgd klecnadngs isectsasvc
     1381 dsntnlldat tssvsaksye ssstesnpss nlldykssaa yksaelsrnf enrllaaenl
     1441 ikesklknlv ptgeaskfdr nlsssyrdtd kcekrslisi getlpatisk rrscipslrl
     1501 rsgsltreps ssvvdrrksf adslgvglaa rsvspeksll sklfkgsgnr eneakekeke
     1561 kektqkckqr risrflrpdf fdtpreesqy akekeaqkaa enerrksrfm rrktengavv
     1621 aressveqek qrekelknei nslkrdsrls rdaeesfevk kngdateess kngflhslek
     1681 kleklrsneq seqseqseek kmagsnatmn grvandnare hsappaesss kersstkrav
     1741 svedlsskkk tgqnssrsrv ssvlglfksg dakqparsqn ailsklkrsp pkatkadasl
     1801 aedtvsttst stsasriptk lakcdskatk kiadtkktdr gekmvssdkk sptkeaprai
     1861 sakekrasse keskekmvdr eveekdstag etvekrslmt kakkttrtsa esgsavdadk
     1921 kvkrlvkkpg ensegdsegt kkkkivrvak kivkkssdts edktmteeke ksgkirakkk
     1981 tvtrsemnsg esngsdsvas ngkakengie tldrnetaee trneqqksqe sqrpnrsnlk
     2041 ldlskipqhs frnstpkkds pksespkvsp kstgndpvpd klmeclskvt hrasitgnki
     2101 vidkplrakd vaelkrevte cakiieshaq sepvsqpvdr eivteevesk rtapeaitpa
     2161 vpindsmeka qtpkesitpi dymnelfspv depesfdsws isstelnhar pdlhsptsps
     2221 rflyaksdnp dsvierirrr sfysrfndkr rpsltapppg lslptsatlp rkfsfsghre
     2281 hrdrgskyag tglglmsnsr htgdrsysly gdegvalgrn rsldrsrysd tspyvsdlks
     2341 ptehlptlss sydplrrylr sptfdlsasr sryhsadfad pdlpayrtpp hlsslllndh
     2401 aidthatsss ssslgsstlp rkygsthaep ktiqyyeell apssvdylts arrsplssey
     2461 lnrcengyyn gnleaqstda krkpmmeftd qsteqirskt erarspsttt eagtvptddh
     2521 s
//
LOCUS       XP_034186746            2521 aa            linear   INV 03-SEP-2025
DEFINITION  nuak family kinase 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186746
VERSION     XP_034186746.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330855.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186746.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2521
                     /product="nuak family kinase 1 isoform X1"
                     /calculated_mol_wt=279256
     Region          32..285
                     /region_name="STKc_NUAK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     novel (nua) kinase family NUAK; cd14073"
                     /db_xref="CDD:270975"
     Site            order(40..44,48,61,63,95,111..114,118,120..121,123,
                     156..157,159,161..162,164,174..175,178,191..196,198,
                     228..231,233)
                     /site_type="active"
                     /db_xref="CDD:270975"
     Site            order(40..43,48,61,63,95,111..114,118,161..162,164,
                     174..175)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270975"
     Site            order(118,120..121,123,157,159,161,178,191..196,198,
                     228..231,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270975"
     Site            174..196
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270975"
     CDS             1..2521
                     /gene="Nuak1"
                     /coded_by="XM_034330855.2:674..8239"
                     /db_xref="GeneID:117607305"
ORIGIN      
        1 mvvgeasihn imggmestgg vrlhnhkrkl kqrfdiikkl gqgtygkvql ginketgqev
       61 aiktikkcki eteadlirir reiqimssvq hpniihiyev fenrekmvlv meyaaggely
      121 dylserkklt eqearrifrq iatavfychk hkichrdlkl enilldqvgn akiadfglsn
      181 vfdeqrllnt fcgsplyasp eivkgtpyhg pevdcwslgv llytlvygam pfdgsnfkrl
      241 vkqisqsdyy epkkpspasp likdmltvrr ckradiekic thwwvnegye qncldiaeel
      301 aaqtpvrldl llslvpqsas aeklvvgdqq tagdvpndms senlvptrch svgslmeldq
      361 nnsdrrirel leeeprtapi tgeakrklet tpsmdetaaa gakrkersrk kekpeerepr
      421 tyksasrhhs apipnaitee amevdptvtv pinpsvdldt vessaaclel ikecnerfps
      481 kersetpvda rneaekkssk pdeaqmvdea anatpaqptt pvsesvtvsd rndasasnda
      541 inleatqref kklkekalsl dselssetsd vpgktferrr skifetaekf nqmasnvdne
      601 kpkkifipgv nvggakrvfe rkaslssast ppptkpsask iiidvptdak knekvkqepe
      661 gqaeeekkne dakkravdii tgaigkppvq krangsppts ppsqepkklp lkipvgtndl
      721 rsatvsvstp vetkfpfedk esdrnkrati stsvsgsana essnvddsqd epkmsskmei
      781 tlksatlprp rkttkaeisl sstkpqeipv afkseveaki dafqpqklrt qrsevafpva
      841 aavpqanrss slepegrprs tapkeriipi qvetgnqqte hsstpppskl ppmpqrsisq
      901 rsgslsrqst adsdtdsalg stvgpepirk spreyiipia vegggyvtpr sgsvepeskt
      961 stptsanapr srfgrprrms sllsdasede spfstlhrds edllqrhmhr lrssrpsrqp
     1021 pehadslssg edddddgfel ltaenlfstl lsrvrsltqr lnvddnrttg fprsrlfdrl
     1081 gsnpsqafwg lnkplssyqt yvetrtvttr lsesqfrhsl sregdvfsrk dpvnssnpat
     1141 pktpnspgsh gtpsretvfd sgnvtlprdk verqdveaep arvrketqes leqsftpsla
     1201 rrlsrtlieq dtgtgsrspy etrcgsptge tsslldasea adrsvstvsd qteyrqgags
     1261 rsgvrransl lersierlgs rresprrsvs lfegngdrey gsedryaegd dfagfskrta
     1321 tgldkwykda agkrnanklr resfrsyrtd kiqeetgdgd klecnadngs isectsasvc
     1381 dsntnlldat tssvsaksye ssstesnpss nlldykssaa yksaelsrnf enrllaaenl
     1441 ikesklknlv ptgeaskfdr nlsssyrdtd kcekrslisi getlpatisk rrscipslrl
     1501 rsgsltreps ssvvdrrksf adslgvglaa rsvspeksll sklfkgsgnr eneakekeke
     1561 kektqkckqr risrflrpdf fdtpreesqy akekeaqkaa enerrksrfm rrktengavv
     1621 aressveqek qrekelknei nslkrdsrls rdaeesfevk kngdateess kngflhslek
     1681 kleklrsneq seqseqseek kmagsnatmn grvandnare hsappaesss kersstkrav
     1741 svedlsskkk tgqnssrsrv ssvlglfksg dakqparsqn ailsklkrsp pkatkadasl
     1801 aedtvsttst stsasriptk lakcdskatk kiadtkktdr gekmvssdkk sptkeaprai
     1861 sakekrasse keskekmvdr eveekdstag etvekrslmt kakkttrtsa esgsavdadk
     1921 kvkrlvkkpg ensegdsegt kkkkivrvak kivkkssdts edktmteeke ksgkirakkk
     1981 tvtrsemnsg esngsdsvas ngkakengie tldrnetaee trneqqksqe sqrpnrsnlk
     2041 ldlskipqhs frnstpkkds pksespkvsp kstgndpvpd klmeclskvt hrasitgnki
     2101 vidkplrakd vaelkrevte cakiieshaq sepvsqpvdr eivteevesk rtapeaitpa
     2161 vpindsmeka qtpkesitpi dymnelfspv depesfdsws isstelnhar pdlhsptsps
     2221 rflyaksdnp dsvierirrr sfysrfndkr rpsltapppg lslptsatlp rkfsfsghre
     2281 hrdrgskyag tglglmsnsr htgdrsysly gdegvalgrn rsldrsrysd tspyvsdlks
     2341 ptehlptlss sydplrrylr sptfdlsasr sryhsadfad pdlpayrtpp hlsslllndh
     2401 aidthatsss ssslgsstlp rkygsthaep ktiqyyeell apssvdylts arrsplssey
     2461 lnrcengyyn gnleaqstda krkpmmeftd qsteqirskt erarspsttt eagtvptddh
     2521 s
//
LOCUS       XP_034186747            2509 aa            linear   INV 03-SEP-2025
DEFINITION  nuak family kinase 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186747
VERSION     XP_034186747.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330856.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186747.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2509
                     /product="nuak family kinase 1 isoform X2"
                     /calculated_mol_wt=277882
     Region          32..285
                     /region_name="STKc_NUAK"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     novel (nua) kinase family NUAK; cd14073"
                     /db_xref="CDD:270975"
     Site            order(40..44,48,61,63,95,111..114,118,120..121,123,
                     156..157,159,161..162,164,174..175,178,191..196,198,
                     228..231,233)
                     /site_type="active"
                     /db_xref="CDD:270975"
     Site            order(40..43,48,61,63,95,111..114,118,161..162,164,
                     174..175)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270975"
     Site            order(118,120..121,123,157,159,161,178,191..196,198,
                     228..231,233)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270975"
     Site            174..196
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270975"
     CDS             1..2509
                     /gene="Nuak1"
                     /coded_by="XM_034330856.2:268..7797"
                     /db_xref="GeneID:117607305"
ORIGIN      
        1 mvvgeasihn imggmestgg vrlhnhkrkl kqrfdiikkl gqgtygkvql ginketgqev
       61 aiktikkcki eteadlirir reiqimssvq hpniihiyev fenrekmvlv meyaaggely
      121 dylserkklt eqearrifrq iatavfychk hkichrdlkl enilldqvgn akiadfglsn
      181 vfdeqrllnt fcgsplyasp eivkgtpyhg pevdcwslgv llytlvygam pfdgsnfkrl
      241 vkqisqsdyy epkkpspasp likdmltvrr ckradiekic thwwvnegye qncldiaeel
      301 aaqtpvrldl llslvpqsas aeklvvgdqq tagdvpndms senlvptrch svgslmeldq
      361 nnsdrrirel leeeprtapi tgeakrklet tpsmdetaaa gakrkersrk kekpeerepr
      421 tyksasrhhs apipnaitee amevdptvtv pinpsvdldt vessaaclel ikecnerfps
      481 kersetpvda rneaekkssk pdeaqmvdea anatpaqptt pvsesvtvsd rndasasnda
      541 inleatqref kklkekalsl dselssetsd vpgktferrr skifetaekf nqmasnvdne
      601 kpkkifipgv nvggakrvfe rkaslssast ppptkpsask iiidvptdak knekvkqepe
      661 gqaeeekkne dakkravdii tgaigkppvq krangsppts ppsqepkklp lkipvgtndl
      721 rsatvsvstp vetkfpfedk esdrnkrati stsvsgsana essnvddsqd epkmsskmei
      781 tlksatlprp rkttkaeisl sstkpqeipv afkseveaki dafqpqklrt qrsevafpva
      841 aavpqanrss slepegrprs tapkeriipi qvetgnqqte hsstpppskl ppmpqrsisq
      901 rsgslsrqst adsdtdsalg stvgpepirk spreyiipia vegggyvtpr sgsvepeskt
      961 stptsanapr srfgrprrms sllsdasede spfstlhrds edllqrhmhr lrssrpsrqp
     1021 pehadslssg edddddgfel ltaenlfstl lsrvrsltqr lnvddnrttg fprsrlfdrl
     1081 gsnpsqafwg lnkplsrrls esqfrhslsr egdvfsrkdp vnssnpatpk tpnspgshgt
     1141 psretvfdsg nvtlprdkve rqdveaepar vrketqesle qsftpslarr lsrtlieqdt
     1201 gtgsrspyet rcgsptgets slldaseaad rsvstvsdqt eyrqgagsrs gvrranslle
     1261 rsierlgsrr esprrsvslf egngdreygs edryaegddf agfskrtatg ldkwykdaag
     1321 krnanklrre sfrsyrtdki qeetgdgdkl ecnadngsis ectsasvcds ntnlldatts
     1381 svsaksyess stesnpssnl ldykssaayk saelsrnfen rllaaenlik esklknlvpt
     1441 geaskfdrnl sssyrdtdkc ekrslisige tlpatiskrr scipslrlrs gsltrepsss
     1501 vvdrrksfad slgvglaars vspeksllsk lfkgsgnren eakekekeke ktqkckqrri
     1561 srflrpdffd tpreesqyak ekeaqkaaen errksrfmrr ktengavvar essveqekqr
     1621 ekelkneins lkrdsrlsrd aeesfevkkn gdateesskn gflhslekkl eklrsneqse
     1681 qseqseekkm agsnatmngr vandnarehs appaessske rsstkravsv edlsskkktg
     1741 qnssrsrvss vlglfksgda kqparsqnai lsklkrsppk atkadaslae dtvsttstst
     1801 sasriptkla kcdskatkki adtkktdrge kmvssdkksp tkeapraisa kekrasseke
     1861 skekmvdrev eekdstaget vekrslmtka kkttrtsaes gsavdadkkv krlvkkpgen
     1921 segdsegtkk kkivrvakki vkkssdtsed ktmteekeks gkirakkktv trsemnsges
     1981 ngsdsvasng kakengietl drnetaeetr neqqksqesq rpnrsnlkld lskipqhsfr
     2041 nstpkkdspk sespkvspks tgndpvpdkl meclskvthr asitgnkivi dkplrakdva
     2101 elkrevteca kiieshaqse pvsqpvdrei vteeveskrt apeaitpavp indsmekaqt
     2161 pkesitpidy mnelfspvde pesfdswsis stelnharpd lhsptspsrf lyaksdnpds
     2221 vierirrrsf ysrfndkrrp sltapppgls lptsatlprk fsfsghrehr drgskyagtg
     2281 lglmsnsrht gdrsyslygd egvalgrnrs ldrsrysdts pyvsdlkspt ehlptlsssy
     2341 dplrrylrsp tfdlsasrsr yhsadfadpd lpayrtpphl sslllndhai dthatsssss
     2401 slgsstlprk ygsthaepkt iqyyeellap ssvdyltsar rsplsseyln rcengyyngn
     2461 leaqstdakr kpmmeftdqs teqirskter arspstttea gtvptddhs
//
LOCUS       XP_034186749            1557 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607306 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186749
VERSION     XP_034186749.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330858.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186749.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1557
                     /product="uncharacterized protein LOC117607306 isoform X1"
                     /calculated_mol_wt=164427
     Region          719..>1384
                     /region_name="FhaB"
                     /note="Large exoprotein involved in heme utilization or
                     adhesion [Intracellular trafficking, secretion, and
                     vesicular transport]; COG3210"
                     /db_xref="CDD:442443"
     Region          1058..>1541
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     CDS             1..1557
                     /gene="LOC117607306"
                     /coded_by="XM_034330858.2:392..5065"
                     /db_xref="GeneID:117607306"
ORIGIN      
        1 mwdlgnrsvi tlvsvlsgcv flynvwgpil iltislflti yacyslivnd sllsphafll
       61 fdyckhisle vcasvqrifk yiyeyirqfr rivsrrfrer nltkfrmerr rgtryqlsdd
      121 typtrsnsss fgsiaqlspi skdvqktdis nnispknkyy hnsghssydh splgnhtvmp
      181 mfprnkeele nqtfgllspn qslskklspa yrqnhalare dstyynsegs mwdtglspkm
      241 dasiselrga qaiggpllss trynidqriy ndvnspgltt rltkyaaean nklmhqsqyr
      301 vgqfpkvnlh aspvplinak svrpktpvtv rvapphtiry sppskqkifs nshhpdnsys
      361 spsvtqslra iplkrratre dvasdlskkq rteslinkef espdetkqkr sreeeslkse
      421 edaspqskae stnrptkrtk tpscydiins lssskhvvsg vkrkaidfsr sgtpdfekhf
      481 kslesiqnns spslpqvqnv spkcpnqevp ekrnsnvftp pdkiqeqfpl rgilkstnks
      541 ietdpdnkei yskrlnafdk ndrikepvvs tdtgklthkl fmraeperne klrmlveeqg
      601 nirakfttdd veeikkedia dmrqtsmkar lqsmfdaisg kaasqinpdv viqaeevnpv
      661 kpvecpvtca tlnssttttn vnttpistsa vapsfgvnet stkntkhvaf nlpnkevpsn
      721 tniqpsntfe aqtktvppst anqtsnapen mftttsaivs hspatnvnvv nsssatpnfn
      781 fgtstpstat stlftpnavs ssnnapslat sstaslfgnv tsnvsmsspv pvtssmtnak
      841 teaslsfptn vpqtvqnvst ssnlsvgsgn vtaasttani apslttfvaq stvpstgsts
      901 itsnmnaipl rmtssmnvss sapapvipsl atiranmemi skqsvplltf gnksgsppap
      961 kpkgfffgsv gnvsqsgnnt fgslansqqv qstntntgts tttstsmngp alqsssasnv
     1021 visstapnla ktmaatfttp aistfsfrap npwesnktgf sfnnapsatk aptessstpa
     1081 fstgnnnalp sfgslpasnp astaasnppq ftmnanpifg nastssifgt ttpsqsifgt
     1141 psttnvtstt faapkssatp mfgsnvntts safanmntap vfsntaaass sggttctlav
     1201 pvfgsaants apsapattgs lfsganstss apafgttsni fgqvtsssat fknttgvfgs
     1261 nsstpnlfrs pttttstfgt tnvasssnts vpvfgsants snvpapafgt tstvtpvfgs
     1321 qntansatpg ttaptfgtst nifgsssstg lpaygsntgs pfgtsasntf ggqnpstsna
     1381 fgatsnvnan anvnatsnvn vtnantnass vpafgdnkvl pfgaqsstfg sssapntafg
     1441 aatnannenn nagsifsfga nqkqpqqnta fsfgsgnnna nnnattsssa pfqfsgapsn
     1501 laasiggfnf saptttpsin fgttsaptfn atapgmfsig sgftaprsrt arsrkpr
//
LOCUS       XP_034186750            1553 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607306 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186750
VERSION     XP_034186750.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330859.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186750.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1553
                     /product="uncharacterized protein LOC117607306 isoform X2"
                     /calculated_mol_wt=164085
     Region          802..>1173
                     /region_name="COG5099"
                     /note="RNA-binding protein of the Puf family,
                     translational repressor [Translation, ribosomal structure
                     and biogenesis]"
                     /db_xref="CDD:227430"
     Region          1058..>1537
                     /region_name="COG4625"
                     /note="Uncharacterized conserved protein, contains a
                     C-terminal beta-barrel porin domain [Function unknown]"
                     /db_xref="CDD:443664"
     CDS             1..1553
                     /gene="LOC117607306"
                     /coded_by="XM_034330859.2:406..5067"
                     /db_xref="GeneID:117607306"
ORIGIN      
        1 mwdlgnrsvi tlvsvlsgcv flynvwgpil iltislflti yacyslivnd sllsphafll
       61 fdyckhisle vcasvqrifk yiyeyirqfr rivsrrfrer nltkfrmerr rgtryqlsdd
      121 typtrsnsss fgsiaqlspi skdvqktdis nnispknkyy hnsghssydh splgnhtvmp
      181 mfprnkeele nqtfgllspn qslskklspa yrqnhalare dstyynsegs mwdtglspkm
      241 dasiselrga qaiggpllss trynidqriy ndvnspgltt rltkyaaean nklmhqsqyr
      301 vgqfpkvnlh aspvplinak svrpktpvtv rvapphtiry sppskqkifs nshhpdnsys
      361 spsvtqslra iplkrratre dvasdlskkq rteslinkef espdetkqkr sreeeslkse
      421 edaspqskae stnrptkrtk tpscydiins lssskhvvsg vkrkaidfsr sgtpdfekhf
      481 kslesiqnns spslpqvqnv spkcpnqevp ekrnsnvftp pdkiqeqfpl rgilkstnks
      541 ietdpdnkei yskrlnafdk ndrikepvvs tdtgklthkl fmraeperne klrmlveeqg
      601 nirakfttdd veeikkedia dmrqtsmkar lqsmfdaisg kaasqinpdv viqaeevnpv
      661 kpvecpvtca tlnssttttn vnttpistsa vapsfgvnet stkntkhvaf nlpnkevpsn
      721 tniqpsntfe aqtktvppst anqtsnapen mftttsaivs hspatnvnvv nsssatpnfn
      781 fgtstpstat stlftpnavs ssnnapslat sstaslfgnv tsnvsmsspv pvtssmtnak
      841 teaslsfptn vpqtvqnvst ssnlsvgsgn vtaasttani apslttfvaq stvpstgsts
      901 itsnmnaipl rmtssmnvss sapapvipsl atiranmemi skqsvplltf gnksgsppap
      961 kpkgfffgsv gnvsqsgnnt fgslansqqv qstntntgts tttstsmngp alqsssasnv
     1021 visstapnla ktmaatfttp aistfsfrap npwesnktgf sfnnapsatk aptessstpa
     1081 fstgnnnalp sfgslpasnp astaasnppq ftmnanpifg nastssifgt ttpsqsifgt
     1141 psttnvtstt faapkssatp mfgsnvntts safanmntap vfsntaaass sggttctlav
     1201 pvfgsaants apsapattgs lfsganstss apafgttsni fgqvtsssat fknttgvfgs
     1261 nsstpnlfrs pttttstfgt tnvasssnts vpvfgsants snvpapafgt tstvtpvfgs
     1321 qntansatpg ttaptfgtst nifgsssstg lpaygsntgs pfgtsasntf ggqnpstsna
     1381 fgatsnvnan anvnatsnvn vtnantnass vpafgdnkvl pfgaqsstfg sssapntafg
     1441 aatnannenn nagsifsfga nqkqpqqnta fsfgsgnnna nnnattsssa pfqfsgapsn
     1501 lggfnfsapt ttpsinfgtt saptfnatap gmfsigsgft aprsrtarsr kpr
//
LOCUS       XP_076548185            1055 aa            linear   INV 03-SEP-2025
DEFINITION  nostrin isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548185
VERSION     XP_076548185.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692070.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1055
                     /product="nostrin isoform X1"
                     /calculated_mol_wt=118122
     Region          503..742
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(506,509..510,514,516..517,520..521,523..524,
                     534..535,537..538,541..542,544..545,548,578,581..582,702,
                     705..706,708,710,713..714,716..717,720..721,724..725,
                     727..728,731..732,734..735)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          1001..1053
                     /region_name="SH3_Nostrin"
                     /note="Src homology 3 domain of Nitric Oxide Synthase
                     TRaffic INducer; cd11823"
                     /db_xref="CDD:212757"
     Site            order(1006,1008,1011,1015,1033..1034,1047,1049..1050)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212757"
     CDS             1..1055
                     /gene="Nost"
                     /coded_by="XM_076692070.1:1223..4390"
                     /db_xref="GeneID:117607307"
ORIGIN      
        1 meearhawas lksirklssl sngnnegngs sttiwydvpv pviihchefp eardtsnasn
       61 eqeareeeac qteeqvvsee ifetgdvret tmermdsgek rhrntvelta vssvktdedg
      121 vpeirfsflr angssvvldr ssvseaeetv tgigkatvtv tatatdtldn sqdastqnrl
      181 ydfpkslhva deietekvve srreaeahre qirgtieesr vsdtivkers lvdsedtlvd
      241 ekcvtskmss tdgkdrydra krlvrslkyg rkrygesdsy dagiasmsgs ysdpecsndt
      301 lssnsprwkn grqklqkhrf gkawdkmrnw lrdettrine vvnkharlqa vgaltgearv
      361 psknssasyd ltkastqelg sitkveefgl asvseenair ssedsditli ktskssnnlr
      421 qvvgssndvf qeegkekivp issysmdklc sdteheehai veiennrdha rkaglikrrm
      481 lgsirglmas thllhvhepe eggqggfedv rryvkqggdf ckelasilhe raeleanyak
      541 glsklsiklt kacakdqggn saggvneawr cvgeemeaaa eahriwgltl geqlakplrv
      601 gvaeaqgrsr ksiensvdka gkslhewrnv atkskkqsfa sarenerlqd larlqsisqs
      661 qqsnnkssnh hmtekeaskl earrrkaeds srradtefyt vsvraerarl eyestmrkga
      721 kqmelleeer lsalkdlanv ylahlqalap rlqqsadrla apvrncnvsq dieilknlir
      781 rvesndgcns dqllpdfyae hvtlamnrer rkqalvrvlh lirqdldrer rgregletlh
      841 rafvatpafa adestqnvte klhhmrsmll yleaarykvg gtlaevegsk rgkhplaehi
      901 lvsrdkqglq qsvlkipswa knesfeiqdd edeidvhlsk tdhdtdsrhw adrtagdgns
      961 enppdeddfs dfdefsshse dnnnqdvdaa ettsksdgte qcraiyqysa nlndelslnp
     1021 gdlitvhqkq pdgwwigecr grtgifpaty vqvih
//
LOCUS       XP_034186752            1053 aa            linear   INV 03-SEP-2025
DEFINITION  nostrin isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186752
VERSION     XP_034186752.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330861.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186752.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1053
                     /product="nostrin isoform X2"
                     /calculated_mol_wt=117881
     Region          501..740
                     /region_name="BAR"
                     /note="The Bin/Amphiphysin/Rvs (BAR) domain, a
                     dimerization module that binds membranes and detects
                     membrane curvature; cl12013"
                     /db_xref="CDD:472257"
     Site            order(504,507..508,512,514..515,518..519,521..522,
                     532..533,535..536,539..540,542..543,546,576,579..580,700,
                     703..704,706,708,711..712,714..715,718..719,722..723,
                     725..726,729..730,732..733)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:153271"
     Region          999..1051
                     /region_name="SH3_Nostrin"
                     /note="Src homology 3 domain of Nitric Oxide Synthase
                     TRaffic INducer; cd11823"
                     /db_xref="CDD:212757"
     Site            order(1004,1006,1009,1013,1031..1032,1045,1047..1048)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212757"
     CDS             1..1053
                     /gene="Nost"
                     /coded_by="XM_034330861.2:2055..5216"
                     /db_xref="GeneID:117607307"
ORIGIN      
        1 meearhawas lksirklssl sngnnegngs sttiwydvpv pviihchefp eardtsnasn
       61 eqeareeeac qteeqvvsee ifetgdvret tmermdsgek rhrntvelta vssvktdedg
      121 vpeirfsflr angssvvldr ssvseaeetv tgigkatvtv tatatdtldn sqdastqnrl
      181 ydfpkslhva deietekvve srreaeahre qirgtieesr vsdtivkers lvdsedtlvd
      241 ekcvtskmss tdgkdrydra krlvrslkyg rkrygesdsy dagiasmsgs ysdpecsndt
      301 lssnsprwkn grqkkhrfgk awdkmrnwlr dettrinevv nkharlqavg altgearvps
      361 knssasydlt kastqelgsi tkveefglas vseenairss edsditlikt skssnnlrqv
      421 vgssndvfqe egkekivpis sysmdklcsd teheehaive iennrdhark aglikrrmlg
      481 sirglmasth llhvhepeeg gqggfedvrr yvkqggdfck elasilhera eleanyakgl
      541 sklsikltka cakdqggnsa ggvneawrcv geemeaaaea hriwgltlge qlakplrvgv
      601 aeaqgrsrks iensvdkagk slhewrnvat kskkqsfasa renerlqdla rlqsisqsqq
      661 snnkssnhhm tekeasklea rrrkaedssr radtefytvs vraerarley estmrkgakq
      721 melleeerls alkdlanvyl ahlqalaprl qqsadrlaap vrncnvsqdi eilknlirrv
      781 esndgcnsdq llpdfyaehv tlamnrerrk qalvrvlhli rqdldrerrg regletlhra
      841 fvatpafaad estqnvtekl hhmrsmllyl eaarykvggt laevegskrg khplaehilv
      901 srdkqglqqs vlkipswakn esfeiqdded eidvhlsktd hdtdsrhwad rtagdgnsen
      961 ppdeddfsdf defsshsedn nnqdvdaaet tsksdgteqc raiyqysanl ndelslnpgd
     1021 litvhqkqpd gwwigecrgr tgifpatyvq vih
//
LOCUS       XP_076548187             951 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glutamate receptor 6 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548187
VERSION     XP_076548187.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692072.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..951
                     /product="metabotropic glutamate receptor 6 isoform X1"
                     /calculated_mol_wt=106553
     Region          34..563
                     /region_name="PBP1_mGluR"
                     /note="ligand binding domain of metabotropic glutamate
                     receptors (mGluR); cd06362"
                     /db_xref="CDD:380585"
     Site            order(61,172,193..195,243,325,438)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380585"
     Site            order(100,103,177,180,203,267)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380585"
     Region          577..627
                     /region_name="NCD3G"
                     /note="Nine Cysteines Domain of family 3 GPCR; pfam07562"
                     /db_xref="CDD:462210"
     Region          647..898
                     /region_name="7tmC_mGluRs"
                     /note="metabotropic glutamate receptors, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15045"
                     /db_xref="CDD:320173"
     Region          647..672
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(650,653..654,657..658,660..661,695,699,702..703,711,
                     715)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320173"
     Region          684..705
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(704,716..717,720..721,724,803,808,811..812,815,849,
                     852..853,856,860,869..870,873,876,880)
                     /site_type="other"
                     /note="allosteric modulator binding site [chemical
                     binding]"
                     /db_xref="CDD:320173"
     Region          714..738
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320173"
     Region          760..780
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320173"
     Region          804..830
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320173"
     Region          838..861
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320173"
     Region          868..892
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320173"
     CDS             1..951
                     /gene="LOC117607308"
                     /coded_by="XM_076692072.1:1..2856"
                     /db_xref="GeneID:117607308"
ORIGIN      
        1 mirtylasll afglsvrrps vgdgrgiaya nvtgdfllgg lfpihrrgtg gescgeiqte
       61 dgvqpleaml ytvnrvnenp kilpgiklgv lafdtcdnps haleqafyfv kgkdklyspv
      121 wfleifsfag fitrseefgq rggeyqcedr sapkfldggf drviailgaq sssvtiqias
      181 vlalfpvpqi symatspfls skeryphffr tvpndvnqar amleilrqfe wsyvsvvysd
      241 seygdhgyet lvslageysi cfsapqrisq drftiddydn vvrtiaekte vrvvvlfaek
      301 sttlrvleaa rrvgvgsrfv wlgsdswpdg rrgmesqeia vlegalavqp lytplggfde
      361 yftglkldhe ridpwfrefw kkyhdceddt pptwkrvsri gdwnlrritv nvkiqarvrc
      421 edprlkidrs tgykqrrflh fvrdavyaaa yalhdmhkds cgesfpgvce emlpinerlf
      481 srylaqvsfk gtqtlvgske vpkrnlltsd eagngfgfvd evdgpprysi lnyqrkgngs
      541 yhwvvvgnyt qneegqsvlr ldrenlrfrg drhaafpsss csrpcgsnri mvrvkedpcc
      601 wrcqscgryr ykvdeqrcee cewgsrptan gtscepipeq fvdysnpwai vatavavsgi
      661 altlfvcsvf wkyrdtpmik asgrelsfll llgtfgcfsm tfavvakptv hscavvrfgi
      721 gfcyticyaa latkinrihr ifndparspr krrytsprsq laiasilglv evafdaswll
      781 keppavthsy psreanvrvc kgsedgsyvi gllypfvltv astiyaiktr kcpegfnetr
      841 riafanyati ilwlafvply lastsnvvkv itlalslsln gmvqllclfl pkiyvvlirp
      901 ekntrelvma rhagsshpta patpiapiap lrpvtpeats pspslvpret v
//
LOCUS       XP_076548188             933 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glutamate receptor 6 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548188
VERSION     XP_076548188.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692073.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..933
                     /product="metabotropic glutamate receptor 6 isoform X2"
                     /calculated_mol_wt=104424
     Region          34..545
                     /region_name="PBP1_mGluR"
                     /note="ligand binding domain of metabotropic glutamate
                     receptors (mGluR); cd06362"
                     /db_xref="CDD:380585"
     Site            order(61,154,175..177,225,307,420)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380585"
     Site            order(100,103,159,162,185,249)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380585"
     Region          559..609
                     /region_name="NCD3G"
                     /note="Nine Cysteines Domain of family 3 GPCR; pfam07562"
                     /db_xref="CDD:462210"
     Region          629..880
                     /region_name="7tmC_mGluRs"
                     /note="metabotropic glutamate receptors, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15045"
                     /db_xref="CDD:320173"
     Region          629..654
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(632,635..636,639..640,642..643,677,681,684..685,693,
                     697)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320173"
     Region          666..687
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(686,698..699,702..703,706,785,790,793..794,797,831,
                     834..835,838,842,851..852,855,858,862)
                     /site_type="other"
                     /note="allosteric modulator binding site [chemical
                     binding]"
                     /db_xref="CDD:320173"
     Region          696..720
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320173"
     Region          742..762
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320173"
     Region          786..812
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320173"
     Region          820..843
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320173"
     Region          850..874
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320173"
     CDS             1..933
                     /gene="LOC117607308"
                     /coded_by="XM_076692073.1:1..2802"
                     /db_xref="GeneID:117607308"
ORIGIN      
        1 mirtylasll afglsvrrps vgdgrgiaya nvtgdfllgg lfpihrrgtg gescgeiqte
       61 dgvqpleaml ytvnrvnenp kilpgiklgv lafdtcdnps haleqafyfv kgfitrseef
      121 gqrggeyqce drsapkfldg gfdrviailg aqsssvtiqi asvlalfpvp qisymatspf
      181 lsskeryphf frtvpndvnq aramleilrq fewsyvsvvy sdseygdhgy etlvslagey
      241 sicfsapqri sqdrftiddy dnvvrtiaek tevrvvvlfa eksttlrvle aarrvgvgsr
      301 fvwlgsdswp dgrrgmesqe iavlegalav qplytplggf deyftglkld heridpwfre
      361 fwkkyhdced dtpptwkrvs rigdwnlrri tvnvkiqarv rcedprlkid rstgykqrrf
      421 lhfvrdavya aayalhdmhk dscgesfpgv ceemlpiner lfsrylaqvs fkgtqtlvgs
      481 kevpkrnllt sdeagngfgf vdevdgppry silnyqrkgn gsyhwvvvgn ytqneegqsv
      541 lrldrenlrf rgdrhaafps sscsrpcgsn rimvrvkedp ccwrcqscgr yrykvdeqrc
      601 eecewgsrpt angtscepip eqfvdysnpw aivatavavs gialtlfvcs vfwkyrdtpm
      661 ikasgrelsf llllgtfgcf smtfavvakp tvhscavvrf gigfcyticy aalatkinri
      721 hrifndpars prkrrytspr sqlaiasilg lvevafdasw llkeppavth sypsreanvr
      781 vckgsedgsy vigllypfvl tvastiyaik trkcpegfne trriafanya tiilwlafvp
      841 lylastsnvv kvitlalsls lngmvqllcl flpkiyvvli rpekntrelv marhagsshp
      901 tapatpiapi aplrpvtpea tspspslvpr etv
//
LOCUS       XP_034186753             932 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glutamate receptor 6 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186753
VERSION     XP_034186753.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330862.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186753.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..932
                     /product="metabotropic glutamate receptor 6 isoform X3"
                     /calculated_mol_wt=104304
     Region          34..544
                     /region_name="PBP1_mGluR"
                     /note="ligand binding domain of metabotropic glutamate
                     receptors (mGluR); cd06362"
                     /db_xref="CDD:380585"
     Site            order(61,172,193..195,243,325,419)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380585"
     Site            order(100,103,177,180,203,267)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380585"
     Region          558..608
                     /region_name="NCD3G"
                     /note="Nine Cysteines Domain of family 3 GPCR; pfam07562"
                     /db_xref="CDD:462210"
     Region          628..879
                     /region_name="7tmC_mGluRs"
                     /note="metabotropic glutamate receptors, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15045"
                     /db_xref="CDD:320173"
     Region          628..653
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(631,634..635,638..639,641..642,676,680,683..684,692,
                     696)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320173"
     Region          665..686
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(685,697..698,701..702,705,784,789,792..793,796,830,
                     833..834,837,841,850..851,854,857,861)
                     /site_type="other"
                     /note="allosteric modulator binding site [chemical
                     binding]"
                     /db_xref="CDD:320173"
     Region          695..719
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320173"
     Region          741..761
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320173"
     Region          785..811
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320173"
     Region          819..842
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320173"
     Region          849..873
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320173"
     CDS             1..932
                     /gene="LOC117607308"
                     /coded_by="XM_034330862.2:1..2799"
                     /db_xref="GeneID:117607308"
ORIGIN      
        1 mirtylasll afglsvrrps vgdgrgiaya nvtgdfllgg lfpihrrgtg gescgeiqte
       61 dgvqpleaml ytvnrvnenp kilpgiklgv lafdtcdnps haleqafyfv kgkdklyspv
      121 wfleifsfag fitrseefgq rggeyqcedr sapkfldggf drviailgaq sssvtiqias
      181 vlalfpvpqi symatspfls skeryphffr tvpndvnqar amleilrqfe wsyvsvvysd
      241 seygdhgyet lvslageysi cfsapqrisq drftiddydn vvrtiaekte vrvvvlfaek
      301 sttlrvleaa rrvgvgsrfv wlgsdswpdg rrgmesqeia vlegalavqp lytplggfde
      361 yftglkldhe ridpwfrefw kkyhdceddt pptwkrarvr cedprlkidr stgykqrrfl
      421 hfvrdavyaa ayalhdmhkd scgesfpgvc eemlpinerl fsrylaqvsf kgtqtlvgsk
      481 evpkrnllts deagngfgfv devdgpprys ilnyqrkgng syhwvvvgny tqneegqsvl
      541 rldrenlrfr gdrhaafpss scsrpcgsnr imvrvkedpc cwrcqscgry rykvdeqrce
      601 ecewgsrpta ngtscepipe qfvdysnpwa ivatavavsg ialtlfvcsv fwkyrdtpmi
      661 kasgrelsfl lllgtfgcfs mtfavvakpt vhscavvrfg igfcyticya alatkinrih
      721 rifndparsp rkrrytsprs qlaiasilgl vevafdaswl lkeppavths ypsreanvrv
      781 ckgsedgsyv igllypfvlt vastiyaikt rkcpegfnet rriafanyat iilwlafvpl
      841 ylastsnvvk vitlalslsl ngmvqllclf lpkiyvvlir pekntrelvm arhagsshpt
      901 apatpiapia plrpvtpeat spspslvpre tv
//
LOCUS       XP_076548189             932 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glutamate receptor 6 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548189
VERSION     XP_076548189.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692074.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..932
                     /product="metabotropic glutamate receptor 6 isoform X4"
                     /calculated_mol_wt=104543
     Region          34..544
                     /region_name="PBP1_mGluR"
                     /note="ligand binding domain of metabotropic glutamate
                     receptors (mGluR); cd06362"
                     /db_xref="CDD:380585"
     Site            order(61,172,193..195,243,325,438)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380585"
     Site            order(100,103,177,180,203,267)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380585"
     Region          558..608
                     /region_name="NCD3G"
                     /note="Nine Cysteines Domain of family 3 GPCR; pfam07562"
                     /db_xref="CDD:462210"
     Region          628..879
                     /region_name="7tmC_mGluRs"
                     /note="metabotropic glutamate receptors, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15045"
                     /db_xref="CDD:320173"
     Region          628..653
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(631,634..635,638..639,641..642,676,680,683..684,692,
                     696)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320173"
     Region          665..686
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(685,697..698,701..702,705,784,789,792..793,796,830,
                     833..834,837,841,850..851,854,857,861)
                     /site_type="other"
                     /note="allosteric modulator binding site [chemical
                     binding]"
                     /db_xref="CDD:320173"
     Region          695..719
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320173"
     Region          741..761
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320173"
     Region          785..811
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320173"
     Region          819..842
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320173"
     Region          849..873
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320173"
     CDS             1..932
                     /gene="LOC117607308"
                     /coded_by="XM_076692074.1:1..2799"
                     /db_xref="GeneID:117607308"
ORIGIN      
        1 mirtylasll afglsvrrps vgdgrgiaya nvtgdfllgg lfpihrrgtg gescgeiqte
       61 dgvqpleaml ytvnrvnenp kilpgiklgv lafdtcdnps haleqafyfv kgkdklyspv
      121 wfleifsfag fitrseefgq rggeyqcedr sapkfldggf drviailgaq sssvtiqias
      181 vlalfpvpqi symatspfls skeryphffr tvpndvnqar amleilrqfe wsyvsvvysd
      241 seygdhgyet lvslageysi cfsapqrisq drftiddydn vvrtiaekte vrvvvlfaek
      301 sttlrvleaa rrvgvgsrfv wlgsdswpdg rrgmesqeia vlegalavqp lytplggfde
      361 yftglkldhe ridpwfrefw kkyhdceddt pptwkrvsri gdwnlrritv nvkiqarvrc
      421 edprlkidrs tgykqrrflh fvrdavyaaa yalhdmhkds cgesfpgvce emlpinerlf
      481 srylaqvsfk deagngfgfv devdgpprys ilnyqrkgng syhwvvvgny tqneegqsvl
      541 rldrenlrfr gdrhaafpss scsrpcgsnr imvrvkedpc cwrcqscgry rykvdeqrce
      601 ecewgsrpta ngtscepipe qfvdysnpwa ivatavavsg ialtlfvcsv fwkyrdtpmi
      661 kasgrelsfl lllgtfgcfs mtfavvakpt vhscavvrfg igfcyticya alatkinrih
      721 rifndparsp rkrrytsprs qlaiasilgl vevafdaswl lkeppavths ypsreanvrv
      781 ckgsedgsyv igllypfvlt vastiyaikt rkcpegfnet rriafanyat iilwlafvpl
      841 ylastsnvvk vitlalslsl ngmvqllclf lpkiyvvlir pekntrelvm arhagsshpt
      901 apatpiapia plrpvtpeat spspslvpre tv
//
LOCUS       XP_076548190             914 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glutamate receptor 6 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548190
VERSION     XP_076548190.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692075.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..914
                     /product="metabotropic glutamate receptor 6 isoform X5"
                     /calculated_mol_wt=102414
     Region          34..526
                     /region_name="PBP1_mGluR"
                     /note="ligand binding domain of metabotropic glutamate
                     receptors (mGluR); cd06362"
                     /db_xref="CDD:380585"
     Site            order(61,154,175..177,225,307,420)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380585"
     Site            order(100,103,159,162,185,249)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380585"
     Region          540..590
                     /region_name="NCD3G"
                     /note="Nine Cysteines Domain of family 3 GPCR; pfam07562"
                     /db_xref="CDD:462210"
     Region          610..861
                     /region_name="7tmC_mGluRs"
                     /note="metabotropic glutamate receptors, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15045"
                     /db_xref="CDD:320173"
     Region          610..635
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(613,616..617,620..621,623..624,658,662,665..666,674,
                     678)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320173"
     Region          647..668
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(667,679..680,683..684,687,766,771,774..775,778,812,
                     815..816,819,823,832..833,836,839,843)
                     /site_type="other"
                     /note="allosteric modulator binding site [chemical
                     binding]"
                     /db_xref="CDD:320173"
     Region          677..701
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320173"
     Region          723..743
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320173"
     Region          767..793
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320173"
     Region          801..824
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320173"
     Region          831..855
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320173"
     CDS             1..914
                     /gene="LOC117607308"
                     /coded_by="XM_076692075.1:1..2745"
                     /db_xref="GeneID:117607308"
ORIGIN      
        1 mirtylasll afglsvrrps vgdgrgiaya nvtgdfllgg lfpihrrgtg gescgeiqte
       61 dgvqpleaml ytvnrvnenp kilpgiklgv lafdtcdnps haleqafyfv kgfitrseef
      121 gqrggeyqce drsapkfldg gfdrviailg aqsssvtiqi asvlalfpvp qisymatspf
      181 lsskeryphf frtvpndvnq aramleilrq fewsyvsvvy sdseygdhgy etlvslagey
      241 sicfsapqri sqdrftiddy dnvvrtiaek tevrvvvlfa eksttlrvle aarrvgvgsr
      301 fvwlgsdswp dgrrgmesqe iavlegalav qplytplggf deyftglkld heridpwfre
      361 fwkkyhdced dtpptwkrvs rigdwnlrri tvnvkiqarv rcedprlkid rstgykqrrf
      421 lhfvrdavya aayalhdmhk dscgesfpgv ceemlpiner lfsrylaqvs fkdeagngfg
      481 fvdevdgppr ysilnyqrkg ngsyhwvvvg nytqneegqs vlrldrenlr frgdrhaafp
      541 ssscsrpcgs nrimvrvked pccwrcqscg ryrykvdeqr ceecewgsrp tangtscepi
      601 peqfvdysnp waivatavav sgialtlfvc svfwkyrdtp mikasgrels fllllgtfgc
      661 fsmtfavvak ptvhscavvr fgigfcytic yaalatkinr ihrifndpar sprkrrytsp
      721 rsqlaiasil glvevafdas wllkeppavt hsypsreanv rvckgsedgs yvigllypfv
      781 ltvastiyai ktrkcpegfn etrriafany atiilwlafv plylastsnv vkvitlalsl
      841 slngmvqllc lflpkiyvvl irpekntrel vmarhagssh ptapatpiap iaplrpvtpe
      901 atspspslvp retv
//
LOCUS       XP_034186755             895 aa            linear   INV 03-SEP-2025
DEFINITION  metabotropic glutamate receptor 6 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186755
VERSION     XP_034186755.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330864.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186755.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..895
                     /product="metabotropic glutamate receptor 6 isoform X6"
                     /calculated_mol_wt=100164
     Region          34..507
                     /region_name="PBP1_mGluR"
                     /note="ligand binding domain of metabotropic glutamate
                     receptors (mGluR); cd06362"
                     /db_xref="CDD:380585"
     Site            order(61,154,175..177,225,307,401)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380585"
     Site            order(100,103,159,162,185,249)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:380585"
     Region          521..571
                     /region_name="NCD3G"
                     /note="Nine Cysteines Domain of family 3 GPCR; pfam07562"
                     /db_xref="CDD:462210"
     Region          591..842
                     /region_name="7tmC_mGluRs"
                     /note="metabotropic glutamate receptors, member of the
                     class C family of seven-transmembrane G protein-coupled
                     receptors; cd15045"
                     /db_xref="CDD:320173"
     Region          591..616
                     /region_name="TM helix 1"
                     /note="TM helix 1 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(594,597..598,601..602,604..605,639,643,646..647,655,
                     659)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:320173"
     Region          628..649
                     /region_name="TM helix 2"
                     /note="TM helix 2 [structural motif]"
                     /db_xref="CDD:320173"
     Site            order(648,660..661,664..665,668,747,752,755..756,759,793,
                     796..797,800,804,813..814,817,820,824)
                     /site_type="other"
                     /note="allosteric modulator binding site [chemical
                     binding]"
                     /db_xref="CDD:320173"
     Region          658..682
                     /region_name="TM helix 3"
                     /note="TM helix 3 [structural motif]"
                     /db_xref="CDD:320173"
     Region          704..724
                     /region_name="TM helix 4"
                     /note="TM helix 4 [structural motif]"
                     /db_xref="CDD:320173"
     Region          748..774
                     /region_name="TM helix 5"
                     /note="TM helix 5 [structural motif]"
                     /db_xref="CDD:320173"
     Region          782..805
                     /region_name="TM helix 6"
                     /note="TM helix 6 [structural motif]"
                     /db_xref="CDD:320173"
     Region          812..836
                     /region_name="TM helix 7"
                     /note="TM helix 7 [structural motif]"
                     /db_xref="CDD:320173"
     CDS             1..895
                     /gene="LOC117607308"
                     /coded_by="XM_034330864.2:1..2688"
                     /db_xref="GeneID:117607308"
ORIGIN      
        1 mirtylasll afglsvrrps vgdgrgiaya nvtgdfllgg lfpihrrgtg gescgeiqte
       61 dgvqpleaml ytvnrvnenp kilpgiklgv lafdtcdnps haleqafyfv kgfitrseef
      121 gqrggeyqce drsapkfldg gfdrviailg aqsssvtiqi asvlalfpvp qisymatspf
      181 lsskeryphf frtvpndvnq aramleilrq fewsyvsvvy sdseygdhgy etlvslagey
      241 sicfsapqri sqdrftiddy dnvvrtiaek tevrvvvlfa eksttlrvle aarrvgvgsr
      301 fvwlgsdswp dgrrgmesqe iavlegalav qplytplggf deyftglkld heridpwfre
      361 fwkkyhdced dtpptwkrar vrcedprlki drstgykqrr flhfvrdavy aaayalhdmh
      421 kdscgesfpg vceemlpine rlfsrylaqv sfkdeagngf gfvdevdgpp rysilnyqrk
      481 gngsyhwvvv gnytqneegq svlrldrenl rfrgdrhaaf pssscsrpcg snrimvrvke
      541 dpccwrcqsc gryrykvdeq rceecewgsr ptangtscep ipeqfvdysn pwaivatava
      601 vsgialtlfv csvfwkyrdt pmikasgrel sfllllgtfg cfsmtfavva kptvhscavv
      661 rfgigfcyti cyaalatkin rihrifndpa rsprkrryts prsqlaiasi lglvevafda
      721 swllkeppav thsypsrean vrvckgsedg syvigllypf vltvastiya iktrkcpegf
      781 netrriafan yatiilwlaf vplylastsn vvkvitlals lslngmvqll clflpkiyvv
      841 lirpekntre lvmarhagss hptapatpia piaplrpvtp eatspspslv pretv
//
LOCUS       XP_034186981             831 aa            linear   INV 03-SEP-2025
DEFINITION  exosome component Rrp6 [Osmia lignaria lignaria].
ACCESSION   XP_034186981
VERSION     XP_034186981.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331090.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186981.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="exosome component Rrp6"
                     /calculated_mol_wt=96361
     Region          50..133
                     /region_name="PMC2NT"
                     /note="PMC2NT (NUC016) domain; pfam08066"
                     /db_xref="CDD:462352"
     Region          256..446
                     /region_name="Rrp6p_like_exo"
                     /note="DEDDy 3'-5' exonuclease domain of yeast Rrp6p,
                     human polymyositis/scleroderma autoantigen 100kDa, and
                     similar proteins; cd06147"
                     /db_xref="CDD:99850"
     Site            order(285..288,338..339,341..343,374..375,408,412)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:99850"
     Site            order(285,287,343,408,412)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99850"
     Site            order(286..288,338..339,341..342,374..375,408,412)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:99850"
     Region          475..555
                     /region_name="HRDC"
                     /note="HRDC domain; cl02578"
                     /db_xref="CDD:470625"
     CDS             1..831
                     /gene="Rrp6"
                     /coded_by="XM_034331090.2:399..2894"
                     /db_xref="GeneID:117607420"
ORIGIN      
        1 mdptedwngd cqtssenqtq enerkevqqi ipgyanfhdy lqesfdvira gikaannlpi
       61 gsdfryyscf psfvnakdrn vkralsvmqc vmatagiknn isnqavdekf dllletndil
      121 ldranelmdk esgitrssev elvvthankq vvngswnsei crppqpadna qsvrllagrn
      181 vqrpqlmfkd kidnrpkpwc pkikdkpnsl kplsiyveeg engevfshpy eyeldmfspp
      241 asqlqksqpt kyksleetpl viiensvdik llledlkryk eiavdlehhs yrsfqgitcl
      301 mqistgdtdy lidtlslrse lhelneiftk psilkvfhga dldiqwlqrd lslyivnmfd
      361 thqaakqlnl pylslayllk qyccidpnkh fqladwrirp lpeelqkyar edthyllyik
      421 dilrnalidl angqmnilks vydrstdick ktyikpiwte escmsiyrks qkmfnnkqly
      481 alielhkwrd ttareeddst ayvlpnhmll niaetlprem qgilaccnpi pplvrqnllk
      541 lhkiilkare qplikpvlge lrqrlnqrsq msnseawmys phdipndmea radlpclldn
      601 vnvvetplat ikhrvtvfds patsedegtl knqknikkkk fifvspfery krvipvvaee
      661 earerekqkq edeerleklk dteaeivesk krvfehfkqv sqdrsetspt krpskaplsq
      721 mkgfkrkrgs nidkekatqe qnmqesivrf qnnnvtnsir srkkgekkrv ikdlnkqhmm
      781 pskrfdykav dfssfqggsk napgqgkqvn feqkmpkkek krkrkkqkin i
//
LOCUS       XP_076548191             831 aa            linear   INV 03-SEP-2025
DEFINITION  exosome component Rrp6 [Osmia lignaria lignaria].
ACCESSION   XP_076548191
VERSION     XP_076548191.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692076.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="exosome component Rrp6"
                     /calculated_mol_wt=96361
     Region          50..133
                     /region_name="PMC2NT"
                     /note="PMC2NT (NUC016) domain; pfam08066"
                     /db_xref="CDD:462352"
     Region          256..446
                     /region_name="Rrp6p_like_exo"
                     /note="DEDDy 3'-5' exonuclease domain of yeast Rrp6p,
                     human polymyositis/scleroderma autoantigen 100kDa, and
                     similar proteins; cd06147"
                     /db_xref="CDD:99850"
     Site            order(285..288,338..339,341..343,374..375,408,412)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:99850"
     Site            order(285,287,343,408,412)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99850"
     Site            order(286..288,338..339,341..342,374..375,408,412)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:99850"
     Region          475..555
                     /region_name="HRDC"
                     /note="HRDC domain; cl02578"
                     /db_xref="CDD:470625"
     CDS             1..831
                     /gene="Rrp6"
                     /coded_by="XM_076692076.1:209..2704"
                     /db_xref="GeneID:117607420"
ORIGIN      
        1 mdptedwngd cqtssenqtq enerkevqqi ipgyanfhdy lqesfdvira gikaannlpi
       61 gsdfryyscf psfvnakdrn vkralsvmqc vmatagiknn isnqavdekf dllletndil
      121 ldranelmdk esgitrssev elvvthankq vvngswnsei crppqpadna qsvrllagrn
      181 vqrpqlmfkd kidnrpkpwc pkikdkpnsl kplsiyveeg engevfshpy eyeldmfspp
      241 asqlqksqpt kyksleetpl viiensvdik llledlkryk eiavdlehhs yrsfqgitcl
      301 mqistgdtdy lidtlslrse lhelneiftk psilkvfhga dldiqwlqrd lslyivnmfd
      361 thqaakqlnl pylslayllk qyccidpnkh fqladwrirp lpeelqkyar edthyllyik
      421 dilrnalidl angqmnilks vydrstdick ktyikpiwte escmsiyrks qkmfnnkqly
      481 alielhkwrd ttareeddst ayvlpnhmll niaetlprem qgilaccnpi pplvrqnllk
      541 lhkiilkare qplikpvlge lrqrlnqrsq msnseawmys phdipndmea radlpclldn
      601 vnvvetplat ikhrvtvfds patsedegtl knqknikkkk fifvspfery krvipvvaee
      661 earerekqkq edeerleklk dteaeivesk krvfehfkqv sqdrsetspt krpskaplsq
      721 mkgfkrkrgs nidkekatqe qnmqesivrf qnnnvtnsir srkkgekkrv ikdlnkqhmm
      781 pskrfdykav dfssfqggsk napgqgkqvn feqkmpkkek krkrkkqkin i
//
LOCUS       XP_076548192             831 aa            linear   INV 03-SEP-2025
DEFINITION  exosome component Rrp6 [Osmia lignaria lignaria].
ACCESSION   XP_076548192
VERSION     XP_076548192.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692077.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="exosome component Rrp6"
                     /calculated_mol_wt=96361
     Region          50..133
                     /region_name="PMC2NT"
                     /note="PMC2NT (NUC016) domain; pfam08066"
                     /db_xref="CDD:462352"
     Region          256..446
                     /region_name="Rrp6p_like_exo"
                     /note="DEDDy 3'-5' exonuclease domain of yeast Rrp6p,
                     human polymyositis/scleroderma autoantigen 100kDa, and
                     similar proteins; cd06147"
                     /db_xref="CDD:99850"
     Site            order(285..288,338..339,341..343,374..375,408,412)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:99850"
     Site            order(285,287,343,408,412)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99850"
     Site            order(286..288,338..339,341..342,374..375,408,412)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:99850"
     Region          475..555
                     /region_name="HRDC"
                     /note="HRDC domain; cl02578"
                     /db_xref="CDD:470625"
     CDS             1..831
                     /gene="Rrp6"
                     /coded_by="XM_076692077.1:193..2688"
                     /db_xref="GeneID:117607420"
ORIGIN      
        1 mdptedwngd cqtssenqtq enerkevqqi ipgyanfhdy lqesfdvira gikaannlpi
       61 gsdfryyscf psfvnakdrn vkralsvmqc vmatagiknn isnqavdekf dllletndil
      121 ldranelmdk esgitrssev elvvthankq vvngswnsei crppqpadna qsvrllagrn
      181 vqrpqlmfkd kidnrpkpwc pkikdkpnsl kplsiyveeg engevfshpy eyeldmfspp
      241 asqlqksqpt kyksleetpl viiensvdik llledlkryk eiavdlehhs yrsfqgitcl
      301 mqistgdtdy lidtlslrse lhelneiftk psilkvfhga dldiqwlqrd lslyivnmfd
      361 thqaakqlnl pylslayllk qyccidpnkh fqladwrirp lpeelqkyar edthyllyik
      421 dilrnalidl angqmnilks vydrstdick ktyikpiwte escmsiyrks qkmfnnkqly
      481 alielhkwrd ttareeddst ayvlpnhmll niaetlprem qgilaccnpi pplvrqnllk
      541 lhkiilkare qplikpvlge lrqrlnqrsq msnseawmys phdipndmea radlpclldn
      601 vnvvetplat ikhrvtvfds patsedegtl knqknikkkk fifvspfery krvipvvaee
      661 earerekqkq edeerleklk dteaeivesk krvfehfkqv sqdrsetspt krpskaplsq
      721 mkgfkrkrgs nidkekatqe qnmqesivrf qnnnvtnsir srkkgekkrv ikdlnkqhmm
      781 pskrfdykav dfssfqggsk napgqgkqvn feqkmpkkek krkrkkqkin i
//
LOCUS       XP_076548193             831 aa            linear   INV 03-SEP-2025
DEFINITION  exosome component Rrp6 [Osmia lignaria lignaria].
ACCESSION   XP_076548193
VERSION     XP_076548193.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692078.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..831
                     /product="exosome component Rrp6"
                     /calculated_mol_wt=96361
     Region          50..133
                     /region_name="PMC2NT"
                     /note="PMC2NT (NUC016) domain; pfam08066"
                     /db_xref="CDD:462352"
     Region          256..446
                     /region_name="Rrp6p_like_exo"
                     /note="DEDDy 3'-5' exonuclease domain of yeast Rrp6p,
                     human polymyositis/scleroderma autoantigen 100kDa, and
                     similar proteins; cd06147"
                     /db_xref="CDD:99850"
     Site            order(285..288,338..339,341..343,374..375,408,412)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:99850"
     Site            order(285,287,343,408,412)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:99850"
     Site            order(286..288,338..339,341..342,374..375,408,412)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:99850"
     Region          475..555
                     /region_name="HRDC"
                     /note="HRDC domain; cl02578"
                     /db_xref="CDD:470625"
     CDS             1..831
                     /gene="Rrp6"
                     /coded_by="XM_076692078.1:448..2943"
                     /db_xref="GeneID:117607420"
ORIGIN      
        1 mdptedwngd cqtssenqtq enerkevqqi ipgyanfhdy lqesfdvira gikaannlpi
       61 gsdfryyscf psfvnakdrn vkralsvmqc vmatagiknn isnqavdekf dllletndil
      121 ldranelmdk esgitrssev elvvthankq vvngswnsei crppqpadna qsvrllagrn
      181 vqrpqlmfkd kidnrpkpwc pkikdkpnsl kplsiyveeg engevfshpy eyeldmfspp
      241 asqlqksqpt kyksleetpl viiensvdik llledlkryk eiavdlehhs yrsfqgitcl
      301 mqistgdtdy lidtlslrse lhelneiftk psilkvfhga dldiqwlqrd lslyivnmfd
      361 thqaakqlnl pylslayllk qyccidpnkh fqladwrirp lpeelqkyar edthyllyik
      421 dilrnalidl angqmnilks vydrstdick ktyikpiwte escmsiyrks qkmfnnkqly
      481 alielhkwrd ttareeddst ayvlpnhmll niaetlprem qgilaccnpi pplvrqnllk
      541 lhkiilkare qplikpvlge lrqrlnqrsq msnseawmys phdipndmea radlpclldn
      601 vnvvetplat ikhrvtvfds patsedegtl knqknikkkk fifvspfery krvipvvaee
      661 earerekqkq edeerleklk dteaeivesk krvfehfkqv sqdrsetspt krpskaplsq
      721 mkgfkrkrgs nidkekatqe qnmqesivrf qnnnvtnsir srkkgekkrv ikdlnkqhmm
      781 pskrfdykav dfssfqggsk napgqgkqvn feqkmpkkek krkrkkqkin i
//
LOCUS       XP_034186756             746 aa            linear   INV 03-SEP-2025
DEFINITION  growth factor receptor-bound protein 14 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186756
VERSION     XP_034186756.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330865.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..746
                     /product="growth factor receptor-bound protein 14 isoform
                     X1"
                     /calculated_mol_wt=85552
     Region          254..335
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          383..507
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          535..571
                     /region_name="BPS"
                     /note="BPS (Between PH and SH2); pfam08947"
                     /db_xref="CDD:462644"
     Region          595..703
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(608,628,649,651)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(650,683)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..746
                     /gene="LOC117607309"
                     /coded_by="XM_034330865.2:452..2692"
                     /db_xref="GeneID:117607309"
ORIGIN      
        1 mprsrwrsrn antkpkprfs rrvdlrrndt vacfrtldmi gnvvllngtv fqpakqdtvh
       61 difdrfsnkd plcledtvad aletkrneqn dsddattams iptavtatpa stttetetet
      121 etlnatttvs riqdssrdae ettherhkiy seaflkccae lasysdnach rdhrrtswfs
      181 rfrrrcssvr dwlptmcscl dgssvfldrs vtrvgnyhrl ggksnffefd rtmeeskrnr
      241 yvdigddlde eveelrfynd dltwntllve rnlrtvdlcq llkvkrsvsg idwsivetwp
      301 elgiertled hedisavhre mkmfasqher kfffrldylk yefffdpkqf fptdmvafpa
      361 aftdhdgtvt daeialrnyl qqengecpqv fslvwirigh ldawrrthml lrdrklylak
      421 keneqlipla hlldyvvyki tnakkrfkap fewgvclrps cnaeavstva gesglkviaf
      481 hseksrvcwl tamrlakygk qlrenyrafk nkqceqsgge npkeryvnyn vsnesarsrv
      541 amdftgsvgr ivedpkeakn iaesegvnwr rtwrpfsrtp pgcavvrlhg lddgihvlqp
      601 wfhrglkrdm aaaivrdhgs vdgvflvres ksnlgayvlt ykysdrvfha qiqpvfderc
      661 ncwlytldkg itkfydllql iefyqlnagc lptrlthyvq ngvpaavprp edggaspspp
      721 elghrfanvt atgatgfast aghrsi
//
LOCUS       XP_034186758             708 aa            linear   INV 03-SEP-2025
DEFINITION  growth factor receptor-bound protein 14 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186758
VERSION     XP_034186758.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330867.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="growth factor receptor-bound protein 14 isoform
                     X2"
                     /calculated_mol_wt=80908
     Region          216..297
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          345..469
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          497..533
                     /region_name="BPS"
                     /note="BPS (Between PH and SH2); pfam08947"
                     /db_xref="CDD:462644"
     Region          557..665
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(570,590,611,613)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(612,645)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..708
                     /gene="LOC117607309"
                     /coded_by="XM_034330867.2:311..2437"
                     /db_xref="GeneID:117607309"
ORIGIN      
        1 mignvvllng tvfqpakqdt vhdifdrfsn kdplcledtv adaletkrne qndsddatta
       61 msiptavtat pastttetet etetlnattt vsriqdssrd aeettherhk iyseaflkcc
      121 aelasysdna chrdhrrtsw fsrfrrrcss vrdwlptmcs cldgssvfld rsvtrvgnyh
      181 rlggksnffe fdrtmeeskr nryvdigddl deeveelrfy nddltwntll vernlrtvdl
      241 cqllkvkrsv sgidwsivet wpelgiertl edhedisavh remkmfasqh erkfffrldy
      301 lkyefffdpk qffptdmvaf paaftdhdgt vtdaeialrn ylqqengecp qvfslvwiri
      361 ghldawrrth mllrdrklyl akkeneqlip lahlldyvvy kitnakkrfk apfewgvclr
      421 pscnaeavst vagesglkvi afhseksrvc wltamrlaky gkqlrenyra fknkqceqsg
      481 genpkeryvn ynvsnesars rvamdftgsv grivedpkea kniaesegvn wrrtwrpfsr
      541 tppgcavvrl hglddgihvl qpwfhrglkr dmaaaivrdh gsvdgvflvr esksnlgayv
      601 ltykysdrvf haqiqpvfde rcncwlytld kgitkfydll qliefyqlna gclptrlthy
      661 vqngvpaavp rpedggasps ppelghrfan vtatgatgfa staghrsi
//
LOCUS       XP_076548194             708 aa            linear   INV 03-SEP-2025
DEFINITION  growth factor receptor-bound protein 14 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548194
VERSION     XP_076548194.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692079.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="growth factor receptor-bound protein 14 isoform
                     X2"
                     /calculated_mol_wt=80908
     Region          216..297
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          345..469
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          497..533
                     /region_name="BPS"
                     /note="BPS (Between PH and SH2); pfam08947"
                     /db_xref="CDD:462644"
     Region          557..665
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(570,590,611,613)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(612,645)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..708
                     /gene="LOC117607309"
                     /coded_by="XM_076692079.1:407..2533"
                     /db_xref="GeneID:117607309"
ORIGIN      
        1 mignvvllng tvfqpakqdt vhdifdrfsn kdplcledtv adaletkrne qndsddatta
       61 msiptavtat pastttetet etetlnattt vsriqdssrd aeettherhk iyseaflkcc
      121 aelasysdna chrdhrrtsw fsrfrrrcss vrdwlptmcs cldgssvfld rsvtrvgnyh
      181 rlggksnffe fdrtmeeskr nryvdigddl deeveelrfy nddltwntll vernlrtvdl
      241 cqllkvkrsv sgidwsivet wpelgiertl edhedisavh remkmfasqh erkfffrldy
      301 lkyefffdpk qffptdmvaf paaftdhdgt vtdaeialrn ylqqengecp qvfslvwiri
      361 ghldawrrth mllrdrklyl akkeneqlip lahlldyvvy kitnakkrfk apfewgvclr
      421 pscnaeavst vagesglkvi afhseksrvc wltamrlaky gkqlrenyra fknkqceqsg
      481 genpkeryvn ynvsnesars rvamdftgsv grivedpkea kniaesegvn wrrtwrpfsr
      541 tppgcavvrl hglddgihvl qpwfhrglkr dmaaaivrdh gsvdgvflvr esksnlgayv
      601 ltykysdrvf haqiqpvfde rcncwlytld kgitkfydll qliefyqlna gclptrlthy
      661 vqngvpaavp rpedggasps ppelghrfan vtatgatgfa staghrsi
//
LOCUS       XP_076548195             708 aa            linear   INV 03-SEP-2025
DEFINITION  growth factor receptor-bound protein 14 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548195
VERSION     XP_076548195.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692080.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="growth factor receptor-bound protein 14 isoform
                     X2"
                     /calculated_mol_wt=80908
     Region          216..297
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          345..469
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          497..533
                     /region_name="BPS"
                     /note="BPS (Between PH and SH2); pfam08947"
                     /db_xref="CDD:462644"
     Region          557..665
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(570,590,611,613)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(612,645)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..708
                     /gene="LOC117607309"
                     /coded_by="XM_076692080.1:281..2407"
                     /db_xref="GeneID:117607309"
ORIGIN      
        1 mignvvllng tvfqpakqdt vhdifdrfsn kdplcledtv adaletkrne qndsddatta
       61 msiptavtat pastttetet etetlnattt vsriqdssrd aeettherhk iyseaflkcc
      121 aelasysdna chrdhrrtsw fsrfrrrcss vrdwlptmcs cldgssvfld rsvtrvgnyh
      181 rlggksnffe fdrtmeeskr nryvdigddl deeveelrfy nddltwntll vernlrtvdl
      241 cqllkvkrsv sgidwsivet wpelgiertl edhedisavh remkmfasqh erkfffrldy
      301 lkyefffdpk qffptdmvaf paaftdhdgt vtdaeialrn ylqqengecp qvfslvwiri
      361 ghldawrrth mllrdrklyl akkeneqlip lahlldyvvy kitnakkrfk apfewgvclr
      421 pscnaeavst vagesglkvi afhseksrvc wltamrlaky gkqlrenyra fknkqceqsg
      481 genpkeryvn ynvsnesars rvamdftgsv grivedpkea kniaesegvn wrrtwrpfsr
      541 tppgcavvrl hglddgihvl qpwfhrglkr dmaaaivrdh gsvdgvflvr esksnlgayv
      601 ltykysdrvf haqiqpvfde rcncwlytld kgitkfydll qliefyqlna gclptrlthy
      661 vqngvpaavp rpedggasps ppelghrfan vtatgatgfa staghrsi
//
LOCUS       XP_034186759             634 aa            linear   INV 03-SEP-2025
DEFINITION  growth factor receptor-bound protein 7 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186759
VERSION     XP_034186759.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330868.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..634
                     /product="growth factor receptor-bound protein 7 isoform
                     X3"
                     /calculated_mol_wt=72740
     Region          254..335
                     /region_name="Ubl1_cv_Nsp3_N-like"
                     /note="first ubiquitin-like (Ubl) domain located at the
                     N-terminus of coronavirus SARS-CoV non-structural protein
                     3 (Nsp3) and related proteins; cl28922"
                     /db_xref="CDD:475130"
     Region          383..>426
                     /region_name="PH-like"
                     /note="Pleckstrin homology-like domain; cl17171"
                     /db_xref="CDD:473070"
     Region          423..459
                     /region_name="BPS"
                     /note="BPS (Between PH and SH2); pfam08947"
                     /db_xref="CDD:462644"
     Region          483..591
                     /region_name="SH2"
                     /note="Src homology 2 (SH2) domain; cl15255"
                     /db_xref="CDD:472789"
     Site            order(496,516,537,539)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198173"
     Site            order(538,571)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198173"
     CDS             1..634
                     /gene="LOC117607309"
                     /coded_by="XM_034330868.2:471..2375"
                     /db_xref="GeneID:117607309"
ORIGIN      
        1 mprsrwrsrn antkpkprfs rrvdlrrndt vacfrtldmi gnvvllngtv fqpakqdtvh
       61 difdrfsnkd plcledtvad aletkrneqn dsddattams iptavtatpa stttetetet
      121 etlnatttvs riqdssrdae ettherhkiy seaflkccae lasysdnach rdhrrtswfs
      181 rfrrrcssvr dwlptmcscl dgssvfldrs vtrvgnyhrl ggksnffefd rtmeeskrnr
      241 yvdigddlde eveelrfynd dltwntllve rnlrtvdlcq llkvkrsvsg idwsivetwp
      301 elgiertled hedisavhre mkmfasqher kfffrldylk yefffdpkqf fptdmvafpa
      361 aftdhdgtvt daeialrnyl qqengecpqv fslvwirigh ldawrrthml lrdrklylak
      421 kesarsrvam dftgsvgriv edpkeaknia esegvnwrrt wrpfsrtppg cavvrlhgld
      481 dgihvlqpwf hrglkrdmaa aivrdhgsvd gvflvresks nlgayvltyk ysdrvfhaqi
      541 qpvfdercnc wlytldkgit kfydllqlie fyqlnagclp trlthyvqng vpaavprped
      601 ggaspsppel ghrfanvtat gatgfastag hrsi
//
LOCUS       XP_034186761             736 aa            linear   INV 03-SEP-2025
DEFINITION  nitric oxide-associated protein 1 [Osmia lignaria lignaria].
ACCESSION   XP_034186761
VERSION     XP_034186761.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330870.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186761.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..736
                     /product="nitric oxide-associated protein 1"
                     /calculated_mol_wt=85134
     Region          234..710
                     /region_name="GTPase_YqeH"
                     /note="ribosome biogenesis GTPase YqeH; TIGR03597"
                     /db_xref="CDD:213834"
     Region          268..455
                     /region_name="YqeH"
                     /note="Circularly permuted YqeH GTPase; cd01855"
                     /db_xref="CDD:206748"
     Site            order(336..337,339..340,380..382,410..416)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206748"
     Site            336..339
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206748"
     Site            380..382
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206748"
     Site            408..415
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206748"
     Site            444..451
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206748"
     Site            446
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206748"
     CDS             1..736
                     /gene="LOC117607310"
                     /coded_by="XM_034330870.2:1..2211"
                     /db_xref="GeneID:117607310"
ORIGIN      
        1 myisgirpwl yqphsqevyr tlkfryfkqk clsrniqsqa dpkvlalrek llycdyldlh
       61 kvrmgfktrq kvyiqekrfk eklhfeklay epvysvmldn vqqkdnqesl qkeehdanvd
      121 nkkagplymp yartdsyeiv ddvelddtin etsldekyle akknysndrn sleveqdven
      181 dtnnalpege lwkvppnwmm dyeqyddtpl ysewyknygt sdpssksssi pcsgcgallh
      241 ckdpafpgfi pselfsrkke edlklmicqr chflkyykaa lkvnisiedy pkllkviknk
      301 kcaiilvvdl tdfpcsiwpd lksilhpftp iflvgnkidl lpidsssgif lnhvkqlltd
      361 tvvditgvsr qnithvelis aqtkfgverl inklqhkwat rgdvylvgct nvgkstlfnt
      421 llnsdyckaq aidlipratv spwpgttlnl lkfpllypss erlqlrikrl qkerqpkqew
      481 leirkaefkk trdikyatlt dfvgrtfsqr sttvtfdpfg eeshkfmtrk ptldeskeey
      541 kdsrwcydtp gtvqrdqild lltidelvlt lpkitmsprt fdlkpkqtvf vaglgrldyl
      601 dgeyhirctl fsnsrlpiti cytsdadevy srlleteafv vptndperlk vwpkfeskef
      661 kvtgldkqsa advvlsnagw iaitpyvnen velrawtpqg rgiylrtpal lrrsvllrgw
      721 kapgtplyll grkayv
//
LOCUS       XP_034186762             708 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186762
VERSION     XP_034186762.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330871.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186762.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X1"
                     /calculated_mol_wt=79082
     Region          <420..583
                     /region_name="SET_SMYD4"
                     /note="SET domain (including iSET domain and post-SET
                     domain) found in SET and MYND domain-containing protein 4
                     (SMYD4) and similar proteins; cd10536"
                     /db_xref="CDD:380934"
     Site            order(476,482,484,486..492,517..521,529,541,552..557,
                     576..580)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380934"
     Site            order(486..489,517..521,554,576..580)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380934"
     Site            order(498..499,507,517,553..554,556)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:380934"
     Site            order(523,578,580,583)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380934"
     CDS             1..708
                     /gene="LOC117607311"
                     /coded_by="XM_034330871.2:437..2563"
                     /db_xref="GeneID:117607311"
ORIGIN      
        1 meggeeryrn lcssetlrsg kkgffsefge rvalvagdrw iakvfarltn dedriraift
       61 epkinsvale tlnrteplyr akdatasrks rlegfvaata akrtnallcf sqavlraplt
      121 gkceevdrgf tlslallara eilaasgqha lalddlrfae eltvpqqfks elarkkeace
      181 kvireneksl vsakelerrg ewttteskpl ppltegenpg ipgassllem eetvsagkra
      241 iaaktivpgd tlvvehpiaa allpdffgth cqhcfsrsvf llppfsltst tvggnrfrfv
      301 apvgcpncsg vafcgrkcrd aamssyhkye ckilalligs gmsvlsmlal rmvtqngpnk
      361 caeirhaltc rtsnegedrn arsstetkps ksakrrsrkk kwrdsqrrek leedidlray
      421 dlvahekqrs akdfferslm aafmlrclqr vgffekptkd eetpneeeig vaallskhlq
      481 mlqfnahevf etrlgtehwf rgskpvylgv avyptvarfn hdcypavtry fvgrsivira
      541 irrlepgdvv aenygpiftk rtlkerqntl agrywfrcec tacredwplf dglsndlvrl
      601 rcptegctkv hgtprdpnkm ikcsscrrev dlrgsletvr ecetlyargf aamdreqaea
      661 alqallegad kfhrvavpph kdthlaeiaa svcmadngnv wspqnppv
//
LOCUS       XP_076548196             708 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548196
VERSION     XP_076548196.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692081.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..708
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X1"
                     /calculated_mol_wt=79082
     Region          <420..583
                     /region_name="SET_SMYD4"
                     /note="SET domain (including iSET domain and post-SET
                     domain) found in SET and MYND domain-containing protein 4
                     (SMYD4) and similar proteins; cd10536"
                     /db_xref="CDD:380934"
     Site            order(476,482,484,486..492,517..521,529,541,552..557,
                     576..580)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380934"
     Site            order(486..489,517..521,554,576..580)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380934"
     Site            order(498..499,507,517,553..554,556)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:380934"
     Site            order(523,578,580,583)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380934"
     CDS             1..708
                     /gene="LOC117607311"
                     /coded_by="XM_076692081.1:438..2564"
                     /db_xref="GeneID:117607311"
ORIGIN      
        1 meggeeryrn lcssetlrsg kkgffsefge rvalvagdrw iakvfarltn dedriraift
       61 epkinsvale tlnrteplyr akdatasrks rlegfvaata akrtnallcf sqavlraplt
      121 gkceevdrgf tlslallara eilaasgqha lalddlrfae eltvpqqfks elarkkeace
      181 kvireneksl vsakelerrg ewttteskpl ppltegenpg ipgassllem eetvsagkra
      241 iaaktivpgd tlvvehpiaa allpdffgth cqhcfsrsvf llppfsltst tvggnrfrfv
      301 apvgcpncsg vafcgrkcrd aamssyhkye ckilalligs gmsvlsmlal rmvtqngpnk
      361 caeirhaltc rtsnegedrn arsstetkps ksakrrsrkk kwrdsqrrek leedidlray
      421 dlvahekqrs akdfferslm aafmlrclqr vgffekptkd eetpneeeig vaallskhlq
      481 mlqfnahevf etrlgtehwf rgskpvylgv avyptvarfn hdcypavtry fvgrsivira
      541 irrlepgdvv aenygpiftk rtlkerqntl agrywfrcec tacredwplf dglsndlvrl
      601 rcptegctkv hgtprdpnkm ikcsscrrev dlrgsletvr ecetlyargf aamdreqaea
      661 alqallegad kfhrvavpph kdthlaeiaa svcmadngnv wspqnppv
//
LOCUS       XP_034186763             687 aa            linear   INV 03-SEP-2025
DEFINITION  protein-lysine N-methyltransferase SMYD4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186763
VERSION     XP_034186763.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330872.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186763.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..687
                     /product="protein-lysine N-methyltransferase SMYD4 isoform
                     X2"
                     /calculated_mol_wt=76803
     Region          271..310
                     /region_name="zf-MYND"
                     /note="MYND finger; pfam01753"
                     /db_xref="CDD:460312"
     Region          <399..562
                     /region_name="SET_SMYD4"
                     /note="SET domain (including iSET domain and post-SET
                     domain) found in SET and MYND domain-containing protein 4
                     (SMYD4) and similar proteins; cd10536"
                     /db_xref="CDD:380934"
     Site            order(455,461,463,465..471,496..500,508,520,531..536,
                     555..559)
                     /site_type="active"
                     /note="putative active site [active]"
                     /db_xref="CDD:380934"
     Site            order(465..468,496..500,533,555..559)
                     /site_type="other"
                     /note="putative SAM binding site"
                     /db_xref="CDD:380934"
     Site            order(477..478,486,496,532..533,535)
                     /site_type="other"
                     /note="putative polypeptide substrate binding site
                     [polypeptide binding]"
                     /db_xref="CDD:380934"
     Site            order(502,557,559,562)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380934"
     CDS             1..687
                     /gene="LOC117607311"
                     /coded_by="XM_034330872.2:433..2496"
                     /db_xref="GeneID:117607311"
ORIGIN      
        1 meggeeryrn lcssetlrsg kkgffsefge rvalvagdrw iakvfarltn dedriraift
       61 epkinsvale tlnrteplyr akdatasrks rlegfvaata akrtnallcf sqavlraplt
      121 gkceevdrgf tlslallara eilaasgqha lalddlrfae eltvpqqfks elarkkeace
      181 kvireneksl vsakelerrg ewttteskpl ppltegenpg ipgassllem eetvsagkra
      241 iaaktivpgd tlvvehpiaa allpdffgth cqhcfsrfva pvgcpncsgv afcgrkcrda
      301 amssyhkyec kilalligsg msvlsmlalr mvtqngpnkc aeirhaltcr tsnegedrna
      361 rsstetkpsk sakrrsrkkk wrdsqrrekl eedidlrayd lvahekqrsa kdfferslma
      421 afmlrclqrv gffekptkde etpneeeigv aallskhlqm lqfnahevfe trlgtehwfr
      481 gskpvylgva vyptvarfnh dcypavtryf vgrsivirai rrlepgdvva enygpiftkr
      541 tlkerqntla grywfrcect acredwplfd glsndlvrlr cptegctkvh gtprdpnkmi
      601 kcsscrrevd lrgsletvre cetlyargfa amdreqaeaa lqallegadk fhrvavpphk
      661 dthlaeiaas vcmadngnvw spqnppv
//
LOCUS       XP_034186764             612 aa            linear   INV 03-SEP-2025
DEFINITION  ATPase family AAA domain containing bor [Osmia lignaria lignaria].
ACCESSION   XP_034186764
VERSION     XP_034186764.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330873.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..612
                     /product="ATPase family AAA domain containing bor"
                     /calculated_mol_wt=69013
     Region          30..284
                     /region_name="ATAD3_N"
                     /note="ATPase family AAA domain-containing protein 3,
                     N-terminal; pfam12037"
                     /db_xref="CDD:463442"
     Region          322..471
                     /region_name="RecA-like_ATAD3-like"
                     /note="ATPase domains of ATPase AAA-domain protein 3A
                     (ATAD3A), -3B, and -3C, and similar ATPase domains;
                     cd19512"
                     /db_xref="CDD:410920"
     Region          <344..600
                     /region_name="CDC48"
                     /note="AAA family ATPase, CDC48 subfamily; TIGR01243"
                     /db_xref="CDD:273521"
     Site            order(351,355..357,373,375..376,409..410)
                     /site_type="other"
                     /note="putative ATP binding site [chemical binding]"
                     /db_xref="CDD:410920"
     CDS             1..612
                     /gene="bor"
                     /coded_by="XM_034330873.2:1..1839"
                     /db_xref="GeneID:117607312"
ORIGIN      
        1 mswlfgyrna qpqdfsqfaq ppasggaigg gddfhhpppr tqmepyrfds taleraasaa
       61 kelersahak ealelsklqe atkqmerqae vkkyeagieq mkaeqkrveg eerrkslqee
      121 tkqhqmraqy qdqlarkryd dqliqqqrmn denlrrqees vakqeamrka tiehemelrh
      181 rnemkkleae vkakakidre nqdlnleqir vkasekrvtv lesiktagsv lgsgmtaflq
      241 dwdkilaatg glsllafgvy takgstgiaa ryiesrlgkp slvretsrft vldtlqhpiq
      301 avkklkskqt dalsgvvlap kleerlrdia iatkntkqnr gmyrnilmhg ppgtgktmfa
      361 kklaehsgmd yaivtggdla plgrdgvtai hkvfdwaats rkglllfide adaflrkrss
      421 ehisedlram lnaflyrtge qsskfmlvla sntpeqfdwa vndrldemve frlpgreere
      481 rlvrlyfdkf vlqpaiegnk rlkvaqfdyg mlcskvanma egmsgrelak lavtwqaaay
      541 asedgvlteq mildkcteai kqhrqkvqwq seqekqesks iyatekeldv kpikskpmie
      601 saapeassva ta
//
LOCUS       XP_034186765             586 aa            linear   INV 03-SEP-2025
DEFINITION  sodium-coupled monocarboxylate transporter 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186765
VERSION     XP_034186765.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330874.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186765.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="sodium-coupled monocarboxylate transporter 2"
                     /calculated_mol_wt=63665
     Region          16..538
                     /region_name="SLC5sbd_NIS-SMVT"
                     /note="Na(+)/iodide (NIS) and Na(+)/multivitamin (SMVT)
                     cotransporters, and related proteins; solute binding
                     domain; cd11492"
                     /db_xref="CDD:271383"
     Site            order(67,70,352,355..356)
                     /site_type="other"
                     /note="Na binding site [ion binding]"
                     /db_xref="CDD:271383"
     CDS             1..586
                     /gene="LOC117607313"
                     /coded_by="XM_034330874.2:1..1761"
                     /db_xref="GeneID:117607313"
ORIGIN      
        1 mdgqknpspq ksfdlidcav fagmlaisai vgvyqaykar kntdavreyl vggqnmsifp
       61 ismsliasyi sgiailglpa emyvygtqlw cvvigdcfvs ltmayvylpv fyglgitssy
      121 eylklrfnda vrlmgcaifl ikmmlyiplv iyvpalafnq vtginlhaia llvcavcify
      181 ttlgglkavv wtdaiqtivm fggvivvavl gtnraggfed vwkrnrdter ieffdmdpdp
      241 tvrhtfwtvv ignylnwlas csvnqamvqr clampnlkka nvtiaimavg iisivslscy
      301 tgivifanfy dcdpittkqi rkpdqllpff vmemaasipg lpglfvagvf saalstmstg
      361 lnsmagviye dmikpwlrvp lsevaasrtm katvvltgci cvtlvflvek lggliqagks
      421 lsgitagpll giftlgmlfp fanstgalvg glvslnlvaw islgtqaais tgkisfpvkp
      481 vsvdgcpdsl kqhtanltlv vetaikeqpf flyrmsylwy twvgfltail vglfvswisg
      541 ankrkqgdek lyapvirrfl sdaslrtkqq entemenvna tnanna
//
LOCUS       XP_034186767             554 aa            linear   INV 03-SEP-2025
DEFINITION  MAP kinase-interacting serine/threonine-protein kinase 1 isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034186767
VERSION     XP_034186767.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330876.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186767.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..554
                     /product="MAP kinase-interacting serine/threonine-protein
                     kinase 1 isoform X1"
                     /calculated_mol_wt=62351
     Region          92..380
                     /region_name="STKc_Mnk"
                     /note="Catalytic domain of the Serine/Threonine kinases,
                     Mitogen-activated protein kinase signal-integrating
                     kinases; cd14090"
                     /db_xref="CDD:270992"
     Site            order(101..105,109,122,124,154,170..173,177,179,215..216,
                     218,220..221,223,236..237,240,263..267,269)
                     /site_type="active"
                     /db_xref="CDD:270992"
     Site            order(101..104,109,122,124,154,170..173,177,220..221,223,
                     236..237)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270992"
     Site            order(105,177,179,215..216,218,220,240,263..267,269)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270992"
     Site            order(236..245,253..267)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270992"
     Site            order(311,315,323,326)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:270992"
     CDS             1..554
                     /gene="LOC117607314"
                     /coded_by="XM_034330876.2:1068..2732"
                     /db_xref="GeneID:117607314"
ORIGIN      
        1 msggwkssfg ytgcrktaeq icfetlrlek eqgggrriek rntqedatcr etvstttges
       61 msavqarqee arrkrrrkkr sgsslmsscf qelyrltgev lgegayasvq tcrslytdle
      121 yavkiidkip ghararvfke vetfhhcqgh pniiqlieff edeekfylvf ekinggqlls
      181 riqerihfse reasqivrei asalnflhkk giahrdlkpe nilcvypdkl tpikvcdfdl
      241 gsgikfnnsl sspvatpqll tpvgsadfma pevveafige anyydkrcdl wslgvimyil
      301 lcgyppfygn cgsdcgwerg encqacqell ftsiqegrye fpdnewrcis edakdlirgl
      361 lvkeahqrls aesilkhswi npgpssvent ekslttphii rrnnsarels afaesamavn
      421 rvvlqhfsvn ldelaekrep rlstsstddd nhpyghmsds sselsehskn cithssnefe
      481 tnsrrksssv rsstdlsdlk tigknrligk essiqqlfgl sppsesrlmq rrlkarsdll
      541 erqsnkavia spsg
//
LOCUS       XP_034186768             528 aa            linear   INV 03-SEP-2025
DEFINITION  MAP kinase-interacting serine/threonine-protein kinase 1 isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034186768
VERSION     XP_034186768.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330877.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..528
                     /product="MAP kinase-interacting serine/threonine-protein
                     kinase 1 isoform X2"
                     /calculated_mol_wt=59446
     Region          66..354
                     /region_name="STKc_Mnk"
                     /note="Catalytic domain of the Serine/Threonine kinases,
                     Mitogen-activated protein kinase signal-integrating
                     kinases; cd14090"
                     /db_xref="CDD:270992"
     Site            order(75..79,83,96,98,128,144..147,151,153,189..190,192,
                     194..195,197,210..211,214,237..241,243)
                     /site_type="active"
                     /db_xref="CDD:270992"
     Site            order(75..78,83,96,98,128,144..147,151,194..195,197,
                     210..211)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270992"
     Site            order(79,151,153,189..190,192,194,214,237..241,243)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270992"
     Site            order(210..219,227..241)
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270992"
     Site            order(285,289,297,300)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:270992"
     CDS             1..528
                     /gene="LOC117607314"
                     /coded_by="XM_034330877.2:1028..2614"
                     /db_xref="GeneID:117607314"
ORIGIN      
        1 mvekileere dgcqevgred atcretvstt tgesmsavqa rqeearrkrr rkkrsgsslm
       61 sscfqelyrl tgevlgegay asvqtcrsly tdleyavkii dkipgharar vfkevetfhh
      121 cqghpniiql ieffedeekf ylvfekingg qllsriqeri hfsereasqi vreiasalnf
      181 lhkkgiahrd lkpenilcvy pdkltpikvc dfdlgsgikf nnslsspvat pqlltpvgsa
      241 dfmapevvea figeanyydk rcdlwslgvi myillcgypp fygncgsdcg wergencqac
      301 qellftsiqe gryefpdnew rcisedakdl irgllvkeah qrlsaesilk hswinpgpss
      361 venteksltt phiirrnnsa relsafaesa mavnrvvlqh fsvnldelae kreprlstss
      421 tdddnhpygh msdssselse hskncithss nefetnsrrk sssvrsstdl sdlktigknr
      481 ligkessiqq lfglsppses rlmqrrlkar sdllerqsnk aviaspsg
//
LOCUS       XP_034186769             546 aa            linear   INV 03-SEP-2025
DEFINITION  S-phase kinase-associated protein 2 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186769
VERSION     XP_034186769.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330878.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186769.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="S-phase kinase-associated protein 2 isoform X1"
                     /calculated_mol_wt=60762
     Region          223..265
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(226..227,229..231,234..235,238,249..251,253,256)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(226,230,233..234,237..238,242,244..245,249..251,253)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          274..455
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          286..315
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          316..340
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          341..364
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          365..389
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          390..414
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          416..442
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          443..467
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          468..487
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..546
                     /gene="Skp2"
                     /coded_by="XM_034330878.2:29..1669"
                     /db_xref="GeneID:117607315"
ORIGIN      
        1 mnpgvaehtr ldddiienst efsppertis gitmskgsps spwwnskrlr lddtcnnnln
       61 stlnsasknn anwscasdgn lvepemladm gvsmleneas scerannses vertspisse
      121 nsktcnksns fdsqesihra ssfdtvivdr ldqdpyesde ptaysnmkne sgyssrtead
      181 nfintkgtfc kddlnastek ssfeqfclyr rkrksssage dkfnklsdem ilmilkwlpk
      241 kclvrsmlvc krwcqiarde alwsrldlgg kvlnegtlgh ilprgvqilr laqaeiadpv
      301 flensevltd gyvsklqyld lsmavispdg lamllslcky lkklslekci vnrscckais
      361 enndletlnl tmcegmdvec ikdltklknl navnvawcgl dakamtllck slprtitrln
      421 iagcrktmtd dnitdlvksc pdmveldlsd ctmltmstvr cildlskleh lslsrcygip
      481 psayvtlaym psllyldvfg lipesvqktl qvtcgetqln kflyssvarp tvgvrrtsiw
      541 glrvrd
//
LOCUS       XP_034186770             527 aa            linear   INV 03-SEP-2025
DEFINITION  S-phase kinase-associated protein 2 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186770
VERSION     XP_034186770.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330879.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186770.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..527
                     /product="S-phase kinase-associated protein 2 isoform X2"
                     /calculated_mol_wt=58687
     Region          204..246
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(207..208,210..212,215..216,219,230..232,234,237)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(207,211,214..215,218..219,223,225..226,230..232,234)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          255..436
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          267..296
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          297..321
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          322..345
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          346..370
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          371..395
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          397..423
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          424..448
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          449..468
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..527
                     /gene="Skp2"
                     /coded_by="XM_034330879.2:54..1637"
                     /db_xref="GeneID:117607315"
ORIGIN      
        1 mnpgvaehtr ldddiienst efsppeskrl rlddtcnnnl nstlnsaskn nanwscasdg
       61 nlvepemlad mgvsmlenea sscerannse svertspiss ensktcnksn sfdsqesihr
      121 assfdtvivd rldqdpyesd eptaysnmkn esgyssrtea dnfintkgtf ckddlnaste
      181 kssfeqfcly rrkrksssag edkfnklsde milmilkwlp kkclvrsmlv ckrwcqiard
      241 ealwsrldlg gkvlnegtlg hilprgvqil rlaqaeiadp vflensevlt dgyvsklqyl
      301 dlsmavispd glamllslck ylkklslekc ivnrscckai senndletln ltmcegmdve
      361 cikdltklkn lnavnvawcg ldakamtllc kslprtitrl niagcrktmt ddnitdlvks
      421 cpdmveldls dctmltmstv rcildlskle hlslsrcygi ppsayvtlay mpsllyldvf
      481 glipesvqkt lqvtcgetql nkflyssvar ptvgvrrtsi wglrvrd
//
LOCUS       XP_034186771             513 aa            linear   INV 03-SEP-2025
DEFINITION  S-phase kinase-associated protein 2 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186771
VERSION     XP_034186771.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330880.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186771.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..513
                     /product="S-phase kinase-associated protein 2 isoform X3"
                     /calculated_mol_wt=57137
     Region          190..232
                     /region_name="F-box_SF"
                     /note="F-box domain superfamily; cl45894"
                     /db_xref="CDD:459239"
     Site            order(193..194,196..198,201..202,205,216..218,220,223)
                     /site_type="other"
                     /note="F-box motif"
                     /db_xref="CDD:438852"
     Site            order(193,197,200..201,204..205,209,211..212,216..218,220)
                     /site_type="other"
                     /note="Skp1 binding site [polypeptide binding]"
                     /db_xref="CDD:438852"
     Region          241..422
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          253..282
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          283..307
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          308..331
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          332..356
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          357..381
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          383..409
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          410..434
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          435..454
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..513
                     /gene="Skp2"
                     /coded_by="XM_034330880.2:156..1697"
                     /db_xref="GeneID:117607315"
ORIGIN      
        1 mskgspsspw wnskrlrldd tcnnnlnstl nsasknnanw scasdgnlve pemladmgvs
       61 mleneassce rannsesver tspissensk tcnksnsfds qesihrassf dtvivdrldq
      121 dpyesdepta ysnmknesgy ssrteadnfi ntkgtfckdd lnastekssf eqfclyrrkr
      181 ksssagedkf nklsdemilm ilkwlpkkcl vrsmlvckrw cqiardealw srldlggkvl
      241 negtlghilp rgvqilrlaq aeiadpvfle nsevltdgyv sklqyldlsm avispdglam
      301 llslckylkk lslekcivnr scckaisenn dletlnltmc egmdvecikd ltklknlnav
      361 nvawcgldak amtllckslp rtitrlniag crktmtddni tdlvkscpdm veldlsdctm
      421 ltmstvrcil dlsklehlsl srcygippsa yvtlaympsl lyldvfglip esvqktlqvt
      481 cgetqlnkfl yssvarptvg vrrtsiwglr vrd
//
LOCUS       XP_034186773             519 aa            linear   INV 03-SEP-2025
DEFINITION  epithelial membrane protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186773
VERSION     XP_034186773.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330882.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..519
                     /product="epithelial membrane protein isoform X1"
                     /calculated_mol_wt=57034
     Region          24..480
                     /region_name="CD36"
                     /note="CD36 family; pfam01130"
                     /db_xref="CDD:460076"
     CDS             1..519
                     /gene="emp"
                     /coded_by="XM_034330882.2:85..1644"
                     /db_xref="GeneID:117607316"
ORIGIN      
        1 mrvhrgicak lqggflrrww aavavgalmi ivaailaavf pklvdvllnr eialrdggrt
       61 fgwwreppvs pqlsvyiynv tnadgflnda ekptleelgp yvylqhwekv evkfndndtv
      121 syrvkkqfvf spelsvgsed dlvvvpnvpm lsatsqskha arflrlamas imdilrikpf
      181 vevsvgqllw gyedpllkla kdvvpkeqkl pydqfgllyg knstmpdwyt iftgqgdigk
      241 ygvldkwngk sslghwtvpe cdgiagsdgs ifppritkqt vlkifdkdlc ralpltfkee
      301 vttaggvpgy rfvpakdafa spsrlesqrc ycpagppcap egtfnvslcq ydspvlisfp
      361 hfyladprlr eavngisapv qekhqffidv qpkmgaalra karvqinlav sqvrdikqva
      421 sfpdiifpim wfedgidelp aemrslmkma vdvppvaraa vsgalaaigt ivligalvcl
      481 araakrqekl hlsnplpsna tsgksgqlnp afqnssask
//
LOCUS       XP_034186774             515 aa            linear   INV 03-SEP-2025
DEFINITION  epithelial membrane protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186774
VERSION     XP_034186774.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330883.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..515
                     /product="epithelial membrane protein isoform X2"
                     /calculated_mol_wt=56509
     Region          20..476
                     /region_name="CD36"
                     /note="CD36 family; pfam01130"
                     /db_xref="CDD:460076"
     CDS             1..515
                     /gene="emp"
                     /coded_by="XM_034330883.2:68..1615"
                     /db_xref="GeneID:117607316"
ORIGIN      
        1 mqrrargriv rrtmtgaava vgalmiivaa ilaavfpklv dvllnreial rdggrtfgww
       61 reppvspqls vyiynvtnad gflndaekpt leelgpyvyl qhwekvevkf ndndtvsyrv
      121 kkqfvfspel svgseddlvv vpnvpmlsat sqskhaarfl rlamasimdi lrikpfvevs
      181 vgqllwgyed pllklakdvv pkeqklpydq fgllygknst mpdwytiftg qgdigkygvl
      241 dkwngksslg hwtvpecdgi agsdgsifpp ritkqtvlki fdkdlcralp ltfkeevtta
      301 ggvpgyrfvp akdafaspsr lesqrcycpa gppcapegtf nvslcqydsp vlisfphfyl
      361 adprlreavn gisapvqekh qffidvqpkm gaalrakarv qinlavsqvr dikqvasfpd
      421 iifpimwfed gidelpaemr slmkmavdvp pvaraavsga laaigtivli galvclaraa
      481 krqeklhlsn plpsnatsgk sgqlnpafqn ssask
//
LOCUS       XP_034186776             516 aa            linear   INV 03-SEP-2025
DEFINITION  scavenger receptor class B member 1 [Osmia lignaria lignaria].
ACCESSION   XP_034186776
VERSION     XP_034186776.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330885.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186776.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..516
                     /product="scavenger receptor class B member 1"
                     /calculated_mol_wt=59160
     Region          30..484
                     /region_name="CD36"
                     /note="CD36 family; pfam01130"
                     /db_xref="CDD:460076"
     CDS             1..516
                     /gene="LOC117607317"
                     /coded_by="XM_034330885.2:145..1695"
                     /db_xref="GeneID:117607317"
ORIGIN      
        1 mekkkkksla lairkrferk rdsrirwlya ftasisvaif ilfwctnvfh dailsnlelr
       61 ngtptflywq qppvdltvkv yvfnytnlke fesgnatklr vqevgpfvyq eslrrvnvql
      121 henktvtyqe krsfrwisgh senekvivpn vmlisalaks rnlmyimqif mttmlssvga
      181 kpfleltvge ylwgyedelf emaklvaspk qpmpykfgil aikngvsadr itmhtgvddl
      241 rnlgmiqrin gmedqhiwed ehcdkiygtd gsmfpphwie qpnntlyvyv kdacrrlplv
      301 yerrgfangi ptlryklpsn vftpttnkds cfcpkeshds lrrtcppigt lnvsacnfgs
      361 pmlvsfphfy agdeslfqri dglepdqerh esyielhprl givvdtkmrf qlnlevqkav
      421 gvpfsgnmed gsilpliwme sgidempeam qqmfyhshyl vnaieagfqw cslvaailsf
      481 gafltalkrd rpveskpatp ltdrselsel selslp
//
LOCUS       XP_034186799             471 aa            linear   INV 03-SEP-2025
DEFINITION  NADH dehydrogenase (ubiquinone) 49 kDa subunit [Osmia lignaria
            lignaria].
ACCESSION   XP_034186799
VERSION     XP_034186799.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330908.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..471
                     /product="NADH dehydrogenase (ubiquinone) 49 kDa subunit"
                     /calculated_mol_wt=53150
     Region          85..471
                     /region_name="PRK06075"
                     /note="NADH-quinone oxidoreductase subunit D"
                     /db_xref="CDD:180385"
     CDS             1..471
                     /gene="ND-49"
                     /coded_by="XM_034330908.2:48..1463"
                     /db_xref="GeneID:117607330"
ORIGIN      
        1 mavralipta rsakglvglw gekgilaagr agalcnvesq rrhghqwlpd ikefealqae
       61 kiyygvedew ippmmrddvq kqpgiknisf nfgpqhpaah gvlrlileie gekvvradph
      121 igllhrgtek lieyktymqa lpyfdrldyv smmcneqcfs laiekllnie vplrakyirt
      181 mfgeitriln himgvgthal digaltpffw lfeereklme fyervsgarm haayvrpggv
      241 sldlplglmd diyqwcsqyg erldetedll tsnriwmgrt rdigvisaed alnwgfsgvm
      301 lrgsgikwdi rkaapydayd lvdfdvpigi ngdcydrylc riaemreslk iiyqclnqmp
      361 egevriddak ivpprreemk tsmealihhf klftqgfqvp pgctytaiea pkgefgvylv
      421 sdgtskpyrc kikapgfahl aalrhigpsc mladvvaiig tldvvfgeid r
//
LOCUS       XP_076548197             449 aa            linear   INV 03-SEP-2025
DEFINITION  phospholipase A1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548197
VERSION     XP_076548197.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692082.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..449
                     /product="phospholipase A1 isoform X1"
                     /calculated_mol_wt=50751
     Region          95..416
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            214..218
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(216,245,352)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(327..328,349..350)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..449
                     /gene="LOC143306060"
                     /coded_by="XM_076692082.1:1..1350"
                     /db_xref="GeneID:143306060"
ORIGIN      
        1 milfvllfik lsllstvhnq vlkksadisf nytdensltl nsynlsdflf spvkfhtrag
       61 adfephqvdc lglgktlata lewffmskpn gsnalnvqfl lssrkqpdrv evitgkqfgl
      121 ewtdfqivrk tviivhgfls hsqepwissm eksflqwgdv nvvvvdwssg sntwnyykaa
      181 vntkivgyqi arfleyiena tstesnwgpl ylvghslgah icgvaakelk krqskwkier
      241 itgldpaqpc fknadstvkl hksdapfvdi ihtngrllsd iglglpepig wlyfsviltf
      301 lfnyyksffs sghvdfypng grsqpgcvnm nssyfeylpi pitdiknsic shgrsyiylt
      361 eslmsdvknn ctfwahywdl sfrslkqlar escnrnvcte mginainysq rgtffvaisd
      421 svpfcsnntr itdeaitale kdyvdqldd
//
LOCUS       XP_076548198             427 aa            linear   INV 03-SEP-2025
DEFINITION  phospholipase A1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548198
VERSION     XP_076548198.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692083.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..427
                     /product="phospholipase A1 isoform X2"
                     /calculated_mol_wt=48039
     Region          95..394
                     /region_name="Pancreat_lipase_like"
                     /note="Pancreatic lipase-like enzymes. Lipases are
                     esterases that can hydrolyze long-chain acyl-triglycerides
                     into di- and monoglycerides, glycerol, and free fatty
                     acids at a water/lipid interface. A typical feature of
                     lipases is 'interfacial activation,' the...; cd00707"
                     /db_xref="CDD:238363"
     Site            214..218
                     /site_type="active"
                     /note="nucleophilic elbow [active]"
                     /db_xref="CDD:238363"
     Site            order(216,245,330)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238363"
     Site            order(305..306,327..328)
                     /site_type="active"
                     /note="active site flap/lid"
                     /db_xref="CDD:238363"
     CDS             1..427
                     /gene="LOC143306060"
                     /coded_by="XM_076692083.1:1..1284"
                     /db_xref="GeneID:143306060"
ORIGIN      
        1 milfvllfik lsllstvhnq vlkksadisf nytdensltl nsynlsdflf spvkfhtrag
       61 adfephqvdc lglgktlata lewffmskpn gsnalnvqfl lssrkqpdrv evitgkqfgl
      121 ewtdfqivrk tviivhgfls hsqepwissm eksflqwgdv nvvvvdwssg sntwnyykaa
      181 vntkivgyqi arfleyiena tstesnwgpl ylvghslgah icgvaakelk krqskwkier
      241 itgldpaqpc fknadstvkl hksdapfvdi ihtngrllsd iglglpepig hvdfypnggr
      301 sqpgcvnmns syfeylpipi tdiknsicsh grsyiyltes lmsdvknnct fwahywdlsf
      361 rslkqlares cnrnvctemg inainysqrg tffvaisdsv pfcsnntrit deaitalekd
      421 yvdqldd
//
LOCUS       XP_034186777             393 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607318 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186777
VERSION     XP_034186777.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330886.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..393
                     /product="uncharacterized protein LOC117607318 isoform X1"
                     /calculated_mol_wt=44595
     Region          12..152
                     /region_name="SPATA6"
                     /note="Spermatogenesis-assoc protein 6; pfam14909"
                     /db_xref="CDD:464365"
     CDS             1..393
                     /gene="LOC117607318"
                     /coded_by="XM_034330886.2:90..1271"
                     /db_xref="GeneID:117607318"
ORIGIN      
        1 magkgfcvki rlelhaitcp gewlcpngkv alritslgst lesnrvspif pllyhnefnf
       61 kktftrlaal telqrkleqe flyaeliqwv spcndnavil atfetnladl lypvvnykgl
      121 lagidvdllm eptryfpgii apkieistrt vieevlgvcc ssldgayvvn pktidskrtc
      181 ahrkrpvkgi irqrkvchsr akaarvpacr ptrcessnle nsyqgeqpri necyhptrrr
      241 lndrcevgag krnhrlstvc gnlhifescp vcskykcyfs ckdddivhqc kqnslkdvrd
      301 sqsfpvcddr ccctcakddd llsresyial hskgphlsgs kekdckaksi secnalylqq
      361 pdetkhgfyk nlerfykrmh kqarrraqea nvf
//
LOCUS       XP_076548199             350 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607318 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548199
VERSION     XP_076548199.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692084.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="uncharacterized protein LOC117607318 isoform X2"
                     /calculated_mol_wt=39365
     Region          12..152
                     /region_name="SPATA6"
                     /note="Spermatogenesis-assoc protein 6; pfam14909"
                     /db_xref="CDD:464365"
     CDS             1..350
                     /gene="LOC117607318"
                     /coded_by="XM_076692084.1:90..1142"
                     /db_xref="GeneID:117607318"
ORIGIN      
        1 magkgfcvki rlelhaitcp gewlcpngkv alritslgst lesnrvspif pllyhnefnf
       61 kktftrlaal telqrkleqe flyaeliqwv spcndnavil atfetnladl lypvvnykgl
      121 lagidvdllm eptryfpgii apkieistrt vieevlgvcc ssldgayvvn pktidskrtc
      181 ahrkrpvkgi irqrkvchsr akaarvpacr ptrcessnle nsyqgeqpri necyhptrrr
      241 lndrcevgag krnhrlstvc gnlhifescp vcskykcyfs ckdddivhqc kqnslkdvrd
      301 sqsfpvcddr ccctcakddd llsresyial hskgphlsgs kekdckakrv
//
LOCUS       XP_034186795             125 aa            linear   INV 03-SEP-2025
DEFINITION  INO80 complex subunit C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186795
VERSION     XP_034186795.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330904.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="INO80 complex subunit C isoform X1"
                     /calculated_mol_wt=13971
     Region          3..120
                     /region_name="YL1_C"
                     /note="YL1 nuclear protein C-terminal domain; cl02154"
                     /db_xref="CDD:470478"
     CDS             1..125
                     /gene="LOC117607329"
                     /coded_by="XM_034330904.2:259..636"
                     /db_xref="GeneID:117607329"
ORIGIN      
        1 mvneetvefs kksqpifknp vfqqkfrsts ttgkkrtwrs lkqvlaqeri lpwpaevvhy
       61 ssinappsfr pakkysdisg lparytdpqt klyyataeef atvrslpmdi tagylalrga
      121 ssivg
//
LOCUS       XP_034186796             125 aa            linear   INV 03-SEP-2025
DEFINITION  INO80 complex subunit C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186796
VERSION     XP_034186796.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330905.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="INO80 complex subunit C isoform X1"
                     /calculated_mol_wt=13971
     Region          3..120
                     /region_name="YL1_C"
                     /note="YL1 nuclear protein C-terminal domain; cl02154"
                     /db_xref="CDD:470478"
     CDS             1..125
                     /gene="LOC117607329"
                     /coded_by="XM_034330905.2:268..645"
                     /db_xref="GeneID:117607329"
ORIGIN      
        1 mvneetvefs kksqpifknp vfqqkfrsts ttgkkrtwrs lkqvlaqeri lpwpaevvhy
       61 ssinappsfr pakkysdisg lparytdpqt klyyataeef atvrslpmdi tagylalrga
      121 ssivg
//
LOCUS       XP_034186797             125 aa            linear   INV 03-SEP-2025
DEFINITION  INO80 complex subunit C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186797
VERSION     XP_034186797.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330906.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="INO80 complex subunit C isoform X1"
                     /calculated_mol_wt=13971
     Region          3..120
                     /region_name="YL1_C"
                     /note="YL1 nuclear protein C-terminal domain; cl02154"
                     /db_xref="CDD:470478"
     CDS             1..125
                     /gene="LOC117607329"
                     /coded_by="XM_034330906.2:95..472"
                     /db_xref="GeneID:117607329"
ORIGIN      
        1 mvneetvefs kksqpifknp vfqqkfrsts ttgkkrtwrs lkqvlaqeri lpwpaevvhy
       61 ssinappsfr pakkysdisg lparytdpqt klyyataeef atvrslpmdi tagylalrga
      121 ssivg
//
LOCUS       XP_034186798             125 aa            linear   INV 03-SEP-2025
DEFINITION  INO80 complex subunit C isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186798
VERSION     XP_034186798.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330907.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..125
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..125
                     /product="INO80 complex subunit C isoform X1"
                     /calculated_mol_wt=13971
     Region          3..120
                     /region_name="YL1_C"
                     /note="YL1 nuclear protein C-terminal domain; cl02154"
                     /db_xref="CDD:470478"
     CDS             1..125
                     /gene="LOC117607329"
                     /coded_by="XM_034330907.2:351..728"
                     /db_xref="GeneID:117607329"
ORIGIN      
        1 mvneetvefs kksqpifknp vfqqkfrsts ttgkkrtwrs lkqvlaqeri lpwpaevvhy
       61 ssinappsfr pakkysdisg lparytdpqt klyyataeef atvrslpmdi tagylalrga
      121 ssivg
//
LOCUS       XP_076548200             105 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607329 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548200
VERSION     XP_076548200.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692085.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..105
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..105
                     /product="uncharacterized protein LOC117607329 isoform X2"
                     /calculated_mol_wt=12057
     CDS             1..105
                     /gene="LOC117607329"
                     /coded_by="XM_076692085.1:94..411"
                     /db_xref="GeneID:117607329"
ORIGIN      
        1 mqnqmkysqv lchhailcli epskravvlf eddlstviek yllhgykynf esqrdsedcy
       61 iallilsqwa kvlvndmnti mmqticgilg cdvqkilvly npeei
//
LOCUS       XP_034186778             307 aa            linear   INV 03-SEP-2025
DEFINITION  required for cell differentiation 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186778
VERSION     XP_034186778.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330887.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..307
                     /product="required for cell differentiation 1 isoform X1"
                     /calculated_mol_wt=34123
     Region          22..280
                     /region_name="Rcd1"
                     /note="Cell differentiation family, Rcd1-like; pfam04078"
                     /db_xref="CDD:461160"
     CDS             1..307
                     /gene="Rcd-1"
                     /coded_by="XM_034330887.2:499..1422"
                     /db_xref="GeneID:117607319"
ORIGIN      
        1 mssqqspaal qstvdrekvy twiielsnpe trenallels kkrevvpdla pmlwhsfgtt
       61 asllqeiini ypainpatlt ayqsnrvcna lallqcvash petrsaflqa hvplflypfl
      121 htvsktrpfe ylrltslgvi galvktdeqe vitfllttei iplclrimes gselsktvat
      181 filqkilldd sglsyicqty drfshvamil gkmvlslakd psarllkhvv rcylrlsdnp
      241 rallalrqcl pdqlrdntfa tclqedastk hwlnqllknl etgpqpgppa qpgqqdprti
      301 gmsplas
//
LOCUS       XP_034186779             256 aa            linear   INV 03-SEP-2025
DEFINITION  required for cell differentiation 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186779
VERSION     XP_034186779.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330888.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="required for cell differentiation 1 isoform X2"
                     /calculated_mol_wt=28371
     Region          1..229
                     /region_name="Rcd1"
                     /note="Cell differentiation family, Rcd1-like; pfam04078"
                     /db_xref="CDD:461160"
     CDS             1..256
                     /gene="Rcd-1"
                     /coded_by="XM_034330888.2:388..1158"
                     /db_xref="GeneID:117607319"
ORIGIN      
        1 mlwhsfgtta sllqeiiniy painpatlta yqsnrvcnal allqcvashp etrsaflqah
       61 vplflypflh tvsktrpfey lrltslgvig alvktdeqev itflltteii plclrimesg
      121 selsktvatf ilqkilldds glsyicqtyd rfshvamilg kmvlslakdp sarllkhvvr
      181 cylrlsdnpr allalrqclp dqlrdntfat clqedastkh wlnqllknle tgpqpgppaq
      241 pgqqdprtig msplas
//
LOCUS       XP_034186781             282 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607321 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186781
VERSION     XP_034186781.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186781.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="uncharacterized protein LOC117607321 isoform X1"
                     /calculated_mol_wt=30283
     CDS             1..282
                     /gene="LOC117607321"
                     /coded_by="XM_034330890.2:175..1023"
                     /db_xref="GeneID:117607321"
ORIGIN      
        1 mkapigllal alltigaaga alnpngvlcg dvrcttveyc shhdkhckpc snicnvtgrn
       61 yepvecstdc qeylhdqryv lleqyndlrg evgrlwilva vsttaavfsl lssmyllakm
      121 fprwvkmraa larlftgkct kvrretslss gskvstpifq ranngnnrrs hnnnnnkvqe
      181 daesgtvkhn glkltmptis asvapsrepe rgtgnesgsa sgtgsrsgsg sgpchatpnt
      241 tttslsgrhp sedttldyay dnpamtpspe taqlrakres sf
//
LOCUS       XP_034186782             282 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607321 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186782
VERSION     XP_034186782.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330891.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186782.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..282
                     /product="uncharacterized protein LOC117607321 isoform X1"
                     /calculated_mol_wt=30283
     CDS             1..282
                     /gene="LOC117607321"
                     /coded_by="XM_034330891.2:1027..1875"
                     /db_xref="GeneID:117607321"
ORIGIN      
        1 mkapigllal alltigaaga alnpngvlcg dvrcttveyc shhdkhckpc snicnvtgrn
       61 yepvecstdc qeylhdqryv lleqyndlrg evgrlwilva vsttaavfsl lssmyllakm
      121 fprwvkmraa larlftgkct kvrretslss gskvstpifq ranngnnrrs hnnnnnkvqe
      181 daesgtvkhn glkltmptis asvapsrepe rgtgnesgsa sgtgsrsgsg sgpchatpnt
      241 tttslsgrhp sedttldyay dnpamtpspe taqlrakres sf
//
LOCUS       XP_034186784             263 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607321 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186784
VERSION     XP_034186784.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..263
                     /product="uncharacterized protein LOC117607321 isoform X2"
                     /calculated_mol_wt=28222
     CDS             1..263
                     /gene="LOC117607321"
                     /coded_by="XM_034330893.2:174..965"
                     /db_xref="GeneID:117607321"
ORIGIN      
        1 mkapigllal alltigaaga alnpngvlcg dvrcttveyc shhdkhckpc snicnvtgrn
       61 yepvecstdc qeylhdqryv lleqyndlrg evgrlwilva vsttaavfsl lssmyllakm
      121 fprwvkmraa larlftgkct kranngnnrr shnnnnnkvq edaesgtvkh nglkltmpti
      181 sasvapsrep ergtgnesgs asgtgsrsgs gsgpchatpn ttttslsgrh psedttldya
      241 ydnpamtpsp etaqlrakre ssf
//
LOCUS       XP_034186785             277 aa            linear   INV 03-SEP-2025
DEFINITION  Y-box binding protein ypsilon schachtel isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186785
VERSION     XP_034186785.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..277
                     /product="Y-box binding protein ypsilon schachtel isoform
                     X1"
                     /calculated_mol_wt=29814
     Region          24..93
                     /region_name="CSD"
                     /note="'Cold-shock' DNA-binding domain; pfam00313"
                     /db_xref="CDD:278729"
     Site            order(30,39,50,83)
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:239905"
     Site            order(36..42,49..52)
                     /site_type="active"
                     /note="RNA-binding motif [active]"
                     /db_xref="CDD:239905"
     CDS             1..277
                     /gene="yps"
                     /coded_by="XM_034330894.2:101..934"
                     /db_xref="GeneID:117607323"
ORIGIN      
        1 madpekqpeq pkavqqklli akkvtgtvkw fnvksgygfi nrndtkedvf vhqsaivknn
       61 prkavrsvgd gevvefdvvv gdkgheaanv tgpsgepvkg spyaadkrrg hrglhwysnq
      121 rrgngrpqrr pregqegydg egktgdeana gdaggqqrry rvrrqydgyy rgarrsgpsa
      181 qqqgdngeag eqggegggeg gvprgggrgr ggptrryfrr nfrggrgggp prrprsqdgq
      241 vkseqksdap kdkdapaaap apqesqpvqn tttesta
//
LOCUS       XP_034186786             256 aa            linear   INV 03-SEP-2025
DEFINITION  Y-box binding protein ypsilon schachtel isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186786
VERSION     XP_034186786.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330895.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="Y-box binding protein ypsilon schachtel isoform
                     X2"
                     /calculated_mol_wt=27709
     Region          24..93
                     /region_name="CSD"
                     /note="'Cold-shock' DNA-binding domain; pfam00313"
                     /db_xref="CDD:278729"
     Site            order(30,39,50,83)
                     /site_type="DNA binding"
                     /note="DNA-binding site [nucleotide binding]"
                     /db_xref="CDD:239905"
     Site            order(36..42,49..52)
                     /site_type="active"
                     /note="RNA-binding motif [active]"
                     /db_xref="CDD:239905"
     CDS             1..256
                     /gene="yps"
                     /coded_by="XM_034330895.2:101..871"
                     /db_xref="GeneID:117607323"
ORIGIN      
        1 madpekqpeq pkavqqklli akkvtgtvkw fnvksgygfi nrndtkedvf vhqsaivknn
       61 prkavrsvgd gevvefdvvv gdkgheaanv tgpsgepvkg spyaadkrrg hrglhwysnq
      121 rrgngrpqrr pregqegydg egktgdeana gdaggqqrry rvrrqydgyy rgarrsgpsa
      181 qqqgdngeag eqggegggeg gvprgggrgr ggptrryfrr nfrggrgggp prrprsqdgq
      241 vkesqpvqnt ttesta
//
LOCUS       XP_034186787             247 aa            linear   INV 03-SEP-2025
DEFINITION  triose phosphate isomerase [Osmia lignaria lignaria].
ACCESSION   XP_034186787
VERSION     XP_034186787.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330896.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..247
                     /product="triose phosphate isomerase"
                     /calculated_mol_wt=26666
     Region          1..245
                     /region_name="PTZ00333"
                     /note="triosephosphate isomerase; Provisional"
                     /db_xref="CDD:240365"
     Site            order(10,12,94,164,170,210,229,231..232)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238190"
     Site            order(10,13,44..46,48,51,63,81,84..85,96..97)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238190"
     Site            order(12,94,164)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238190"
     CDS             1..247
                     /gene="Tpi"
                     /coded_by="XM_034330896.2:146..889"
                     /db_xref="GeneID:117607324"
ORIGIN      
        1 mgrkffvggn wkmnglktei dgivaflkag pldpnvelvv gvpsiyltyt ksilpsnisi
       61 agqnaykvak gaftgeispa mlmdngipwv ilghserrnv fgetdelvae kvahalesgl
      121 kviacigekl eereagktee vvfrqtkaia dkikswdnvi vayepvwaig tgktatpeqa
      181 qevhlklrew fsknvnptva envriiyggs vtagnakdla kekdidgflv ggaslkpdfv
      241 qivnakq
//
LOCUS       XP_034186788             176 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic prostaglandin E synthase isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186788
VERSION     XP_034186788.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330897.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..176
                     /product="cytosolic prostaglandin E synthase isoform X1"
                     /calculated_mol_wt=19821
     Region          10..113
                     /region_name="p23_hB-ind1_like"
                     /note="p23_like domain found in human (h) butyrate-induced
                     transcript 1 (B-ind1) and similar proteins. hB-ind1
                     participates in signaling by the small GTPase Rac1. It
                     binds to Rac1 and enhances different Rac1 effects
                     including activation of nuclear factor (NF)...; cd06465"
                     /db_xref="CDD:107222"
     Site            order(16,28..29,73,78,93,104,106..107,109..110,112)
                     /site_type="active"
                     /note="Hsp90 binding site [active]"
                     /db_xref="CDD:107222"
     CDS             1..176
                     /gene="p23"
                     /coded_by="XM_034330897.2:144..674"
                     /db_xref="GeneID:117607325"
ORIGIN      
        1 mtqegqlppp pvmwaqrrdi lfvticledc kdpviniepq miyfkgvggt eqkmhevtin
       61 lygeivpnrt vqnlrgrtfe lilikkeegp ywprltkekt kahwlksdfn kwkdeddsdd
      121 eggmegsgnd leemmrqmgg lggsgdskpi fddlddlgde gegmdsdddd lpdlvd
//
LOCUS       XP_034186789             171 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic prostaglandin E synthase isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186789
VERSION     XP_034186789.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330898.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="cytosolic prostaglandin E synthase isoform X2"
                     /calculated_mol_wt=19278
     Region          5..108
                     /region_name="p23_hB-ind1_like"
                     /note="p23_like domain found in human (h) butyrate-induced
                     transcript 1 (B-ind1) and similar proteins. hB-ind1
                     participates in signaling by the small GTPase Rac1. It
                     binds to Rac1 and enhances different Rac1 effects
                     including activation of nuclear factor (NF)...; cd06465"
                     /db_xref="CDD:107222"
     Site            order(11,23..24,68,73,88,99,101..102,104..105,107)
                     /site_type="active"
                     /note="Hsp90 binding site [active]"
                     /db_xref="CDD:107222"
     CDS             1..171
                     /gene="p23"
                     /coded_by="XM_034330898.2:17..532"
                     /db_xref="GeneID:117607325"
ORIGIN      
        1 mlppppvmwa qrrdilfvti cledckdpvi niepqmiyfk gvggteqkmh evtinlygei
       61 vpnrtvqnlr grtfelilik keegpywprl tkektkahwl ksdfnkwkde ddsddeggme
      121 gsgndleemm rqmgglggsg dskpifddld dlgdegegmd sddddlpdlv d
//
LOCUS       XP_034186790             151 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial ribosomal protein L20 [Osmia lignaria lignaria].
ACCESSION   XP_034186790
VERSION     XP_034186790.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330899.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186790.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..151
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..151
                     /product="mitochondrial ribosomal protein L20"
                     /calculated_mol_wt=17095
     Region          13..113
                     /region_name="Ribosomal_L20"
                     /note="Ribosomal protein L20; pfam00453"
                     /db_xref="CDD:459817"
     CDS             1..151
                     /gene="mRpL20"
                     /coded_by="XM_034330899.2:160..615"
                     /db_xref="GeneID:117607326"
ORIGIN      
        1 mvflsarlfp rsrgpdefwr krqifklaah yigrrrncys iairtvtral vyatkgrklk
       61 kedmkelweg rinaaahehg infktlmegl trsniflnrk slstlavwep rtfkslsdia
      121 cakaklggvk gvanntmpkt vickglidel d
//
LOCUS       XP_034186793             133 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607327 [Osmia lignaria lignaria].
ACCESSION   XP_034186793
VERSION     XP_034186793.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330902.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..133
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..133
                     /product="uncharacterized protein LOC117607327"
                     /calculated_mol_wt=15664
     Region          7..107
                     /region_name="FAM183"
                     /note="FAM183A and FAM183B related; pfam14886"
                     /db_xref="CDD:434286"
     CDS             1..133
                     /gene="LOC117607327"
                     /coded_by="XM_034330902.2:3..404"
                     /db_xref="GeneID:117607327"
ORIGIN      
        1 mdplklrvyt erfekekkyi klqeefvaia ksqpltgkfy akhdvipase vqqeyvdlit
       61 krlisvptdi yqfrppsvnm eygwfskpli prtrdprlhf prqqaefivn eilirrsqrg
      121 lpvekftgip fkv
//
LOCUS       XP_034186794             132 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607328 [Osmia lignaria lignaria].
ACCESSION   XP_034186794
VERSION     XP_034186794.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330903.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186794.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..132
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..132
                     /product="uncharacterized protein LOC117607328"
                     /calculated_mol_wt=15701
     CDS             1..132
                     /gene="LOC117607328"
                     /coded_by="XM_034330903.2:690..1088"
                     /db_xref="GeneID:117607328"
ORIGIN      
        1 mdrknypdsi tertsekqpp fklcfskdrl lgrsdlrvlk teddptaskf ystkyesyen
       61 edknekakdq qgniykfinr rspltshayg wwyeqgirpt esridfrkkt sdlvnyqmki
      121 yaedqklkns kh
//
LOCUS       XP_034190773            1955 aa            linear   INV 03-SEP-2025
DEFINITION  collagen type IV alpha 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034190773
VERSION     XP_034190773.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334882.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190773.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1955
                     /product="collagen type IV alpha 1 isoform X1"
                     /calculated_mol_wt=193304
     Region          <256..>554
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <479..>737
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <700..>929
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <990..1304
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1241..1530
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1495..>1728
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1733..1837
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     Region          1841..1953
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     CDS             1..1955
                     /gene="Col4a1"
                     /coded_by="XM_034334882.2:378..6245"
                     /db_xref="GeneID:117609044"
ORIGIN      
        1 mtrlglrfva savflagivs aqfnhtswsn dpppvvplgr gdigkpnstd deyiaprwra
       61 flsnnegsee adrrnlepyd egrnyddgyn rypdggeygq rsgtdgsggy qrgygggdrr
      121 tysqshsqsh sqshsqsqgq gtqdpynqgp vdpygrsrgy edtygrgaev egqrsrdpyg
      181 dgygenrgsg daygredpyg rgysqgqggg ygaagdsdny pssysavpip graqpknctg
      241 saccvpkcfa ekgsrgspgv mgpqgpkgqr gfpgaegllg pkgekgdpgp qgprgpigdr
      301 gkmgmpgfpg ingvpgvqgp pgspglpgrd gcngtdgepg lrglpgepgp rgfrgtpgpk
      361 gdkgqpafag mfpvgqkgep gidgvrgppg ppgpqgehgi pgpkgergpy ggsgipgpkg
      421 ekgnmglgfe gvkgdkgqkg eqgppgssvp lvpfegvppe vtgekgepgg kgdkgemgld
      481 gqkgepglig dhgipgysgg kgekglpgap girgrdgfsg ppgppgrkgd rgydgldgip
      541 grpgqkgqpg rdgpmgtsgl rgppgppggg kgtpgppgpk gpqgfpgpig prgadgypgd
      601 pgprgpigmt ggpgsqgipg peglpgekgg kgepgitgfp gptgprgfdg ppgspgprgl
      661 qgekglsivg pkgmdgapgl dgekgqkger gysgprgfpg dsldgipgsp gesgfpgepg
      721 tpgkdgapgf pgapgekgdi ggrcqdclpg lsglkgdrgl dgipglpgpr gppgergfpg
      781 eagadglpgp igppgppgkd gipgsagpqg vpgtpavvsr ssiklekger glrgepgrpg
      841 ppgpqgppge kgalgfegfp gtkgtagdrg fpgqdgipgr pgvpgrkges gisvkgepge
      901 agrlgapgfk gepglygqkg epgecppltw vkdlkgdrgs qgekgepgpp grdgpsgdkg
      961 lqgfsgppgp pgqigppgpv gprglpgprg dkgdmgpmgf pgepgregpr gfpgapapkg
     1021 dkgepgisvk gspglpgppg ekgekglpgp pgkegqvgfp glpgfmgekg dvgvpgisgl
     1081 pgapggkgdt gpvgprgppg vagipgldgm kgepglpgep graglpgfpg akgdrgepgi
     1141 dgpkgypgrr gppglqgrpg vegasgmkge rgekgssgfp glpgmegrpg ppglsgdkgd
     1201 dglpgppglv gpigfdglkg drgppglpgr kgepgqvsek gqkgepgipg irgppgpsgr
     1261 dgidgakges gipgmgrdgl pgpkgepgip grdglpglqg qkgdtgvrgf pglkgdfgpp
     1321 gasgepgtpg idglpgergd igppgppgfp gldgdmgapg rpglngskge rgfpgpsglp
     1381 giaggpgekg drgapgptiq vkgekgepgi pglsgapgek gdrgleglag ydgekgdrga
     1441 pgpagnpgpp ghsgakgdeg prgpsgisgl tvkgekglpg mpgkhgregi pgrpgekgeq
     1501 glpglpgykg dqgpygpigp qgekgdmgpa gtpglpgrdg rpglegmpgl lgekgdkgdi
     1561 glrglpgipg qkgeagfpgp sgldglpgek gdrgwdglng apgasgekgd rgypgpaglk
     1621 gppgylgpkg dkgedciepp mgpkgdrgyp gptgppgppg ekglpgppgf sgldgvkgas
     1681 glpgppgpag lpglpgpvgs pglpglqgpv gvpgpagepg qpcdvvpdyl tgillvrhsq
     1741 sqtvpvcepg hiklwegysl lytdgderah sqdlgyagsc vrkfstmpfl fcdvnnvchy
     1801 asrndrsywl stnspipmmp ieetaieeyi srcvvcevpa nvlavhsqsl sipecpqgwt
     1861 glwigysfvm htgagsqggg qslsspgscl edfratpfie cngakghchy yanefsfwma
     1921 siedrqqfqr pekqtlkagn lrsrisrcqv ciknt
//
LOCUS       XP_034190774            1954 aa            linear   INV 03-SEP-2025
DEFINITION  collagen type IV alpha 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190774
VERSION     XP_034190774.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334883.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190774.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1954
                     /product="collagen type IV alpha 1 isoform X2"
                     /calculated_mol_wt=193247
     Region          <255..>553
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <478..>736
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <699..>928
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <989..1303
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1240..1529
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1494..>1727
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1732..1836
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     Region          1840..1952
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     CDS             1..1954
                     /gene="Col4a1"
                     /coded_by="XM_034334883.2:378..6242"
                     /db_xref="GeneID:117609044"
ORIGIN      
        1 mtrlglrfva savflagivs aqfnhtswsn dpppvvplgr gdigkpnstd deyiaprwra
       61 flsnnegsee adrrnlepyd egrnyddgyn rypdggeygq rsgtdgsggy qrgyggdrrt
      121 ysqshsqshs qshsqsqgqg tqdpynqgpv dpygrsrgye dtygrgaeve gqrsrdpygd
      181 gygenrgsgd aygredpygr gysqgqgggy gaagdsdnyp ssysavpipg raqpknctgs
      241 accvpkcfae kgsrgspgvm gpqgpkgqrg fpgaegllgp kgekgdpgpq gprgpigdrg
      301 kmgmpgfpgi ngvpgvqgpp gspglpgrdg cngtdgepgl rglpgepgpr gfrgtpgpkg
      361 dkgqpafagm fpvgqkgepg idgvrgppgp pgpqgehgip gpkgergpyg gsgipgpkge
      421 kgnmglgfeg vkgdkgqkge qgppgssvpl vpfegvppev tgekgepggk gdkgemgldg
      481 qkgepgligd hgipgysggk gekglpgapg irgrdgfsgp pgppgrkgdr gydgldgipg
      541 rpgqkgqpgr dgpmgtsglr gppgppgggk gtpgppgpkg pqgfpgpigp rgadgypgdp
      601 gprgpigmtg gpgsqgipgp eglpgekggk gepgitgfpg ptgprgfdgp pgspgprglq
      661 gekglsivgp kgmdgapgld gekgqkgerg ysgprgfpgd sldgipgspg esgfpgepgt
      721 pgkdgapgfp gapgekgdig grcqdclpgl sglkgdrgld gipglpgprg ppgergfpge
      781 agadglpgpi gppgppgkdg ipgsagpqgv pgtpavvsrs siklekgerg lrgepgrpgp
      841 pgpqgppgek galgfegfpg tkgtagdrgf pgqdgipgrp gvpgrkgesg isvkgepgea
      901 grlgapgfkg epglygqkge pgecppltwv kdlkgdrgsq gekgepgppg rdgpsgdkgl
      961 qgfsgppgpp gqigppgpvg prglpgprgd kgdmgpmgfp gepgregprg fpgapapkgd
     1021 kgepgisvkg spglpgppge kgekglpgpp gkegqvgfpg lpgfmgekgd vgvpgisglp
     1081 gapggkgdtg pvgprgppgv agipgldgmk gepglpgepg raglpgfpga kgdrgepgid
     1141 gpkgypgrrg ppglqgrpgv egasgmkger gekgssgfpg lpgmegrpgp pglsgdkgdd
     1201 glpgppglvg pigfdglkgd rgppglpgrk gepgqvsekg qkgepgipgi rgppgpsgrd
     1261 gidgakgesg ipgmgrdglp gpkgepgipg rdglpglqgq kgdtgvrgfp glkgdfgppg
     1321 asgepgtpgi dglpgergdi gppgppgfpg ldgdmgapgr pglngskger gfpgpsglpg
     1381 iaggpgekgd rgapgptiqv kgekgepgip glsgapgekg drgleglagy dgekgdrgap
     1441 gpagnpgppg hsgakgdegp rgpsgisglt vkgekglpgm pgkhgregip grpgekgeqg
     1501 lpglpgykgd qgpygpigpq gekgdmgpag tpglpgrdgr pglegmpgll gekgdkgdig
     1561 lrglpgipgq kgeagfpgps gldglpgekg drgwdglnga pgasgekgdr gypgpaglkg
     1621 ppgylgpkgd kgedcieppm gpkgdrgypg ptgppgppge kglpgppgfs gldgvkgasg
     1681 lpgppgpagl pglpgpvgsp glpglqgpvg vpgpagepgq pcdvvpdylt gillvrhsqs
     1741 qtvpvcepgh iklwegysll ytdgderahs qdlgyagscv rkfstmpflf cdvnnvchya
     1801 srndrsywls tnspipmmpi eetaieeyis rcvvcevpan vlavhsqsls ipecpqgwtg
     1861 lwigysfvmh tgagsqgggq slsspgscle dfratpfiec ngakghchyy anefsfwmas
     1921 iedrqqfqrp ekqtlkagnl rsrisrcqvc iknt
//
LOCUS       XP_034190775            1835 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609045 [Osmia lignaria lignaria].
ACCESSION   XP_034190775
VERSION     XP_034190775.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334884.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190775.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1835
                     /product="uncharacterized protein LOC117609045"
                     /calculated_mol_wt=182802
     Region          <141..>382
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <268..>576
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <602..>858
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <936..>1190
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1098..>1345
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          <1279..>1513
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1540..1645
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     Region          1648..1758
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     CDS             1..1835
                     /gene="LOC117609045"
                     /coded_by="XM_034334884.2:490..5997"
                     /db_xref="GeneID:117609045"
ORIGIN      
        1 mrpraaatst sparglhpfp vvlrvtcvla llvvtwipvt eavicrdpyk cdckgikgip
       61 gfigitgpqg peglpgdigp dgppgpkgek gaggelggtg ekgyrgdqgi qgfpgspgls
      121 gntglagvmg pdgldgcngt dgragspgmp gihgergppg pigdygptge pgdgginsvg
      181 vkgirgnpgv dgpkgypgpy gipgemgypg etgdmgytga pglpgmqgep getvfgvqgq
      241 sgeqgdrgea gpssdgkfrk ttkstpvkgv rgnkgmrgdy gdrgrkgemg akgplgrpgf
      301 pgikgmkgeq gddgprgkqg aegppgpage kgdkgapgya gnpgvdgtdg esgepgnpgp
      361 pgkqgaagep gkyipeldei iqgnigiqgd lgppgkpgep gtpglpgkvg yigppgppgp
      421 sgppgspglk gssvkgepgd dglpgvmgsh gppglpglpg pfgpkgypgi tiigppgidg
      481 kpgypgisgs lgdrgdpglq gpkgfpgkgi rirgppgerg lpgtpgipgr dgrpgefgvf
      541 gpkgirgddc gicspglpgp kgesgdagln gypgtpgfpg lpgprgfkgt qgkpgisgpg
      601 glegdsgrpg iagpqgpkge kgklilpdgv ligepgdfge kglpgiegsg gprgepgsrg
      661 dtgppgpkge kgdhgppgfs grngfpgsdg ipgpkgddal tpieflrgep glpgspglkg
      721 aegdkgakge rgqssqyain lkgdrgykge rgapgedgak gaqgwsgdeg fpglpgipgs
      781 pgispqgpmg lkgypgmpge qgprgpsgtp gpqgpvgfsg rignkgnrgd pgtnqtygny
      841 glmglagekg digspgpqgf qgrsgidgpk gvkggkgapg legqpglqga kgqrgdsvqg
      901 prgfpglagq pgmpgrmgea gipgpdgppg fdgmkglkge vgfigrrgdd gdigpmgylg
      961 rdgtpgmpgp pgeegdvgei gepgprgwpg ldgvaglpgf kgeqgkigvq gvpgglgppg
     1021 fkgasgdagp dgldglpgen sfsgpqgdrg epgfmgkiga pgfpgvdgrp glpgepglat
     1081 dgfdglpgli gepgydgfng vpgpkgesgd mgpdglkgpr gdwgfkgrag tpgihgrygg
     1141 kgergpmgpp gfngpkgrrg ldgdpgqmgl pgmpgdqgpr glpglmgtpg pkgqtgdhgl
     1201 psyaeaekgd fgnpgleglp gvkgqrgdqg yiglpgrkge kgnegfygpd gfpgvpgfpg
     1261 lpgdqgppgf pgapgdfaer geagrpgldg ipgtpgipgq kgapgqygpn gpkgirgeig
     1321 ltirgpkgil gdqgpkgfag fpgapglqgl psppgppgpk gsqgepglpg isikgqqgep
     1381 glpgfnggig skgergdtgl pgfaglpghk gergdegepg vsgmigspgp kgpkgnrgnn
     1441 pfspfprkgl pgdtgpeglp gfpglqgmmg epgldglpgr agepgmigal glpgpegkeg
     1501 pagysgprga vgrqgppgyp gipgapappa pgprnrgfhf arhsqsemip lcpsntvklw
     1561 dgfsllhimg ngkpyaqdlg apgscvrkfs tmpfmfcnin nvcdyanrdd ysywlsttep
     1621 mpmsmtpipa peigryisrc svceaptrai avhsqtmsip qcpggweelw igysflmhrd
     1681 agaggggqsl ispgscleef rarpfiecrs lgtcnyfsta tsywlatikd hemfrkplpq
     1741 tlkndhtsrv srcavcirrr vnedgsqlkp ftsngqketl dniryapqpr ypdyqyprqg
     1801 spgrtrnphr ripyhyrkkg rsrrpdrtte vpeaa
//
LOCUS       XP_034190776            1035 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609047 [Osmia lignaria lignaria].
ACCESSION   XP_034190776
VERSION     XP_034190776.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334885.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190776.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1035
                     /product="uncharacterized protein LOC117609047"
                     /calculated_mol_wt=119928
     Region          73..>171
                     /region_name="Peptidase_M13_N"
                     /note="Peptidase family M13; pfam05649"
                     /db_xref="CDD:461703"
     Region          <531..1032
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(818,823..826,845,861..862,865..866,869,929,974,
                     977..978,995..996,1002)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..1035
                     /gene="LOC117609047"
                     /coded_by="XM_034334885.2:88..3195"
                     /db_xref="GeneID:117609047"
ORIGIN      
        1 mlrllilasl gavinasllp dhilnyprgl qslfgtkret ggeekaernv cnsaeckvia
       61 dllktsmdet vdpcedfyef acgkwsmknp vpenrtewsl wimvsekven qvqsiietkp
      121 kpndlfavkl akkwyescmd tdmierrgae piistlwrhg gwplimeege wderiyhwqi
      181 vddhfarllg fnafhdvrys sltyedndti lidaphlplg vekllsvdai lnnmdssden
      241 nesgegsqea gsrergeeqq qenqeegend eeeeendlst nienrnkmvk rknhkrfghg
      301 rkhisrhgtk krtkrdalke kivhqilhql kkanthkvks skrghlsmis gkssmkkknh
      361 qlhgkmakrk nskrlghgkr knnknkvmik hvhnrnlkkk snvhknkhym qrkinnhrvh
      421 havnkmkiih hkkmslvndt egtdndldnn vdtengneee snndenndes kddiedeend
      481 vdnkndeene enddetnnde eeneednken deeenegede dedekvdiee lkemykefml
      541 nvslilskar gieisretlm kdindlfefs lkmaeltlik ekpvnitlrd fqkeydtmes
      601 ttrngkinwv kkvqkifaea gvdieddidi vvtnpeyike lhalldktps ktivnyihwl
      661 fvsmaiiagp qelrslaenw fprrvhfssr pseciqtvdi sdivayqyvq qyfsedtakt
      721 ardmiddiqk eveyqikest wmnddtkhfi ldklmnmknl vgypswyknt tmvkeyfqgl
      781 tigpsyyenl lnyirhlkwk nlrrivettg rdksseyldp lmlnaffmpt ensisvtaad
      841 fqsplfalnr pwavnfgiig lvmghevnhg fdneghlydh ggnlmewlsa madaynkrae
      901 cfvnqfnkys ivkgenytie nygnqtagen iadtmglqav fraykrrere cgnpdpalpe
      961 lekfsndqif flsfanlwce sidresliqs tkydvhspgr lrvigsvsns edfakafncp
     1021 vgstmnperk cniwv
//
LOCUS       XP_034190777             973 aa            linear   INV 03-SEP-2025
DEFINITION  neprilysin-1-like [Osmia lignaria lignaria].
ACCESSION   XP_034190777
VERSION     XP_034190777.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334886.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190777.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..973
                     /product="neprilysin-1-like"
                     /calculated_mol_wt=113161
     Region          72..>170
                     /region_name="Peptidase_M13_N"
                     /note="Peptidase family M13; pfam05649"
                     /db_xref="CDD:461703"
     Region          <464..970
                     /region_name="M13"
                     /note="Peptidase family M13 includes neprilysin and
                     endothelin-converting enzyme I; cd08662"
                     /db_xref="CDD:341056"
     Site            order(754,759..762,781,797..798,801..802,805,867,912,
                     915..916,933..934,940)
                     /site_type="active"
                     /db_xref="CDD:341056"
     CDS             1..973
                     /gene="LOC117609048"
                     /coded_by="XM_034334886.2:104..3025"
                     /db_xref="GeneID:117609048"
ORIGIN      
        1 mmqiiwifvf fgiiasgtcn ilnhhlnsrw sfgankesit eeskierslc ttkqcqelak
       61 llmegmkkti npcddfyeyv cgnwkihnpl ppdekrwnlv vklqrnvdrr leeilkseat
      121 pedlyavrfs kraynvcmdt demdkaglqp lvstlwragg wplimeeeew deqifkwqti
      181 ddyyaniigl nafhdlhivq ywsskeydii itsphlprmm selitteeid mdssdennds
      241 gegsqekgse eddrkdddes eddeeendek defilhkrki tkkkinkqla nktrinkhik
      301 trtkrsittr gnkhrqisdh kmnklfkykp isqpnsvkes ainynlknkr ehenlnsdly
      361 fndddvehen indddivsys dneeengnie qdkdnkessg nynddeenee sgsgsgdyed
      421 ddddddnddd ddgsgsgngd gegdktdedn sseerrkerm dnikkakelr esyakymfnv
      481 smalakargi lvpeeklkkd vadllefein llkivykyge elnmtlqlfq kkydclvpvt
      541 nngeinwirk venlfisggm eidkktpvvi psfsyiknlk klldktpskt ivnyvhwnfl
      601 srviksttke mrdwywgsei itvkkrsnlc iteigaeysl gyeyvkkyfs dkilqtasdm
      661 iddiekevey qikestwmdi etkhfildkl vylkkligyp ksyrnntimn qlfkglsvsr
      721 shydnilsim rylkwqklkl ifkkeietse efvmnpltin affivfynai evtaadfqsp
      781 ffdshqpwft nfgaigaima hevnhgfdss grvfdkcgey mawlpamaea yekraecfqd
      841 qftkysvvyn ktanipiedy gkqtetenia dtmglqaafr ayqrrqrice epdpmlpgle
      901 nfsneqllfi sfgnlwceva dpeelgkkvr rdshslaplr ligsisnsed faktfncpeg
      961 spmnpkkkcn iwk
//
LOCUS       XP_034190780             233 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609050 [Osmia lignaria lignaria].
ACCESSION   XP_034190780
VERSION     XP_034190780.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334889.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..233
                     /product="uncharacterized protein LOC117609050"
                     /calculated_mol_wt=26691
     CDS             1..233
                     /gene="LOC117609050"
                     /coded_by="XM_034334889.2:68..769"
                     /db_xref="GeneID:117609050"
ORIGIN      
        1 maryssesds dhnsryrrrr srrsrssssd ssdssphrrr sskhskskrr hrshsrsrgr
       61 drdrgqksyk ssrnssskgs erhkyrsrsr ssdqskrkvr sasreksgsr ssssqsnvks
      121 tvsdktktil ikddfpiepr fkegvldein segftpkqft ssntkekkfk nividisadt
      181 iqvptvaeip cgtesifhss imldqevrfd kwvkklytlr qkaiadlihs nvt
//
LOCUS       XP_034190781             218 aa            linear   INV 03-SEP-2025
DEFINITION  RAS oncogene family member Rab14 [Osmia lignaria lignaria].
ACCESSION   XP_034190781
VERSION     XP_034190781.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334890.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..218
                     /product="RAS oncogene family member Rab14"
                     /calculated_mol_wt=24167
     Region          10..175
                     /region_name="Rab14"
                     /note="Rab GTPase family 14 (Rab14); cd04122"
                     /db_xref="CDD:133322"
     Site            12..13
                     /site_type="other"
                     /note="Rab subfamily motif 1 (RabSF1)"
                     /db_xref="CDD:133322"
     Site            18..25
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:133322"
     Site            order(21..26,36,66,124..125,127..128,155..156)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:133322"
     Site            order(26..36,41..42)
                     /site_type="other"
                     /note="Rab subfamily motif 2 (RabSF2)"
                     /db_xref="CDD:133322"
     Site            order(36,41..49)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:133322"
     Site            order(41,45..52,59,61)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:133322"
     Site            43
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:133322"
     Site            order(44,46..48,63,65,72..73,76,80,82..85)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:133322"
     Site            order(44..45,47,65..66,73,75,77..79)
                     /site_type="other"
                     /note="putative GDI interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:133322"
     Site            44..48
                     /site_type="other"
                     /note="Rab family motif 1 (RabF1)"
                     /db_xref="CDD:133322"
     Site            61..65
                     /site_type="other"
                     /note="Rab family motif 2 (RabF2)"
                     /db_xref="CDD:133322"
     Site            66..69
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:133322"
     Site            order(69,71..81)
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:133322"
     Site            72..77
                     /site_type="other"
                     /note="Rab family motif 3 (RabF3)"
                     /db_xref="CDD:133322"
     Site            80..84
                     /site_type="other"
                     /note="Rab family motif 4 (RabF4)"
                     /db_xref="CDD:133322"
     Site            89..94
                     /site_type="other"
                     /note="Rab family motif 5 (RabF5)"
                     /db_xref="CDD:133322"
     Site            112..121
                     /site_type="other"
                     /note="Rab subfamily motif 3 (RabSF3)"
                     /db_xref="CDD:133322"
     Site            124..127
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:133322"
     Site            154..156
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:133322"
     Site            170..175
                     /site_type="other"
                     /note="Rab subfamily motif 4 (RabSF4)"
                     /db_xref="CDD:133322"
     CDS             1..218
                     /gene="Rab14"
                     /coded_by="XM_034334890.2:397..1053"
                     /db_xref="GeneID:117609051"
ORIGIN      
        1 mstgpynysy ifkyiiigdm gvgkscllhq ftekkfmadc phtigvefgt riievagqki
       61 klqiwdtagq erfravtrsy yrgaagalmv yditrrstyn hlsswltdtr nltnpstvif
      121 lignksdleg qrdvtyeeak qfadenglmf veasaktghn veeafletak kifqsiqdgr
      181 ldlnaaesgv qhnpsqpgrt nlqgvsneqq ggkdscsc
//
LOCUS       XP_034190783             202 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609052 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190783
VERSION     XP_034190783.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334892.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190783.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="uncharacterized protein LOC117609052 isoform X1"
                     /calculated_mol_wt=22212
     Region          27..196
                     /region_name="CIA30"
                     /note="Complex I intermediate-associated protein 30
                     (CIA30); cl21728"
                     /db_xref="CDD:473944"
     CDS             1..202
                     /gene="LOC117609052"
                     /coded_by="XM_034334892.2:325..933"
                     /db_xref="GeneID:117609052"
ORIGIN      
        1 mnlqyicgif lvlmichltk gnkeilfdft tadnvdnwte vsdtvrtvgk skavltlqkt
       61 qlfqhaifft llnpqpngag fagvetvtnl nlsnfeniki scrgqgansh ykvvlehkgl
      121 htngnityeq ffvasmsdtd fstvilplas fkpyyrgrev pnaepldtan itmiglqiyg
      181 gvylpikqkg vsalevqtis vs
//
LOCUS       XP_034190784             179 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609052 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190784
VERSION     XP_034190784.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334893.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..179
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..179
                     /product="uncharacterized protein LOC117609052 isoform X2"
                     /calculated_mol_wt=19665
     Region          12..173
                     /region_name="CIA30"
                     /note="Complex I intermediate-associated protein 30
                     (CIA30); cl21728"
                     /db_xref="CDD:473944"
     CDS             1..179
                     /gene="LOC117609052"
                     /coded_by="XM_034334893.2:114..653"
                     /db_xref="GeneID:117609052"
ORIGIN      
        1 milykqltlh nvdnwtevsd tvrtvgkska vltlqktqlf qhaifftlln pqpngagfag
       61 vetvtnlnls nfenikiscr gqganshykv vlehkglhtn gnityeqffv asmsdtdfst
      121 vilplasfkp yyrgrevpna epldtanitm iglqiyggvy lpikqkgvsa levqtisvs
//
LOCUS       XP_034194165             396 aa            linear   INV 03-SEP-2025
DEFINITION  abnormal chemosensory protein 6 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194165
VERSION     XP_034194165.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338274.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="abnormal chemosensory protein 6 isoform X1"
                     /calculated_mol_wt=42468
     Region          234..311
                     /region_name="POU"
                     /note="Found in Pit-Oct-Unc transcription factors;
                     smart00352"
                     /db_xref="CDD:197673"
     Region          333..389
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..396
                     /gene="acj6"
                     /coded_by="XM_034338274.2:923..2113"
                     /db_xref="GeneID:117610649"
ORIGIN      
        1 mmyspdkmmg skglhgapit apgcfpsgry spppyraapd pmpppprrcm pnptsriled
       61 asimcnswsp rhngdlfggl nsglqdglls raealaadlg khnagtpmpl khdpvsvyhh
      121 gahmaaphpn nrphhqvhsh lfmshpgmes ldmldpansm ttltpmsent gggpghhagi
      181 hgpsvyggmn gmmshhhhhg nlgggggsvp hpahhhpama aaaaaaaaag lhpdadtdpr
      241 eleafaerfk qrriklgvtq advgkalanl klpgvgalsq sticrfeslt lshnnmialk
      301 pilqawleea eaqaknkrrd pdapsvlpag ekkrkrtsia apekrsleay favqprpsge
      361 kiaaiaekld lkknvvrvwf cnqrqkqkrm kfaaqh
//
LOCUS       XP_034194166             390 aa            linear   INV 03-SEP-2025
DEFINITION  abnormal chemosensory protein 6 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194166
VERSION     XP_034194166.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338275.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..390
                     /product="abnormal chemosensory protein 6 isoform X2"
                     /calculated_mol_wt=41747
     Region          228..305
                     /region_name="POU"
                     /note="Found in Pit-Oct-Unc transcription factors;
                     smart00352"
                     /db_xref="CDD:197673"
     Region          327..383
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..390
                     /gene="acj6"
                     /coded_by="XM_034338275.2:923..2095"
                     /db_xref="GeneID:117610649"
ORIGIN      
        1 mmyspdkmmg skglhgapit apgcfpsgry spppyraapd pmpppprrcm pnptsriled
       61 asimcnswsp rhngdlfggl nsglqdglls raealaadlg khnagtpmpl khdpvsvyhh
      121 gahmaaphpn nrphhqmshp gmesldmldp ansmttltpm sentgggpgh hagihgpsvy
      181 ggmngmmshh hhhgnlgggg gsvphpahhh pamaaaaaaa aaaglhpdad tdpreleafa
      241 erfkqrrikl gvtqadvgka lanlklpgvg alsqsticrf esltlshnnm ialkpilqaw
      301 leeaeaqakn krrdpdapsv lpagekkrkr tsiaapekrs leayfavqpr psgekiaaia
      361 ekldlkknvv rvwfcnqrqk qkrmkfaaqh
//
LOCUS       XP_076548201             166 aa            linear   INV 03-SEP-2025
DEFINITION  vesicle transport protein SFT2B isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548201
VERSION     XP_076548201.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692086.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..166
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..166
                     /product="vesicle transport protein SFT2B isoform X1"
                     /calculated_mol_wt=18130
     Region          42..151
                     /region_name="Got1"
                     /note="Got1/Sft2-like family; pfam04178"
                     /db_xref="CDD:461210"
     CDS             1..166
                     /gene="LOC117607333"
                     /coded_by="XM_076692086.1:336..836"
                     /db_xref="GeneID:117607333"
ORIGIN      
        1 mdklrralsg ndqcdeesgi itqtdvdfvv mdqstlswst rikgfaicfi vgilcsflgs
       61 falflhkglt ifavfytlgn vislastcfl mgpcsqlkkm fastrivati lvfvaigmtl
      121 faahlknpgl allfiiiqsl amtwyslsyi pyardavkkt vescit
//
LOCUS       XP_034186801             160 aa            linear   INV 03-SEP-2025
DEFINITION  vesicle transport protein SFT2B isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186801
VERSION     XP_034186801.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330910.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..160
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..160
                     /product="vesicle transport protein SFT2B isoform X2"
                     /calculated_mol_wt=17453
     Region          36..145
                     /region_name="Got1"
                     /note="Got1/Sft2-like family; pfam04178"
                     /db_xref="CDD:461210"
     CDS             1..160
                     /gene="LOC117607333"
                     /coded_by="XM_034330910.2:331..813"
                     /db_xref="GeneID:117607333"
ORIGIN      
        1 mdklrralsg ndqcdeesgi itqvmdqstl swstrikgfa icfivgilcs flgsfalflh
       61 kgltifavfy tlgnvislas tcflmgpcsq lkkmfastri vatilvfvai gmtlfaahlk
      121 npglallfii iqslamtwys lsyipyarda vkktvescit
//
LOCUS       XP_034183302             644 aa            linear   INV 03-SEP-2025
DEFINITION  IGF-II mRNA-binding protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183302
VERSION     XP_034183302.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327411.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..644
                     /product="IGF-II mRNA-binding protein isoform X1"
                     /calculated_mol_wt=69110
     Region          4..79
                     /region_name="RRM1_VICKZ"
                     /note="RNA recognition motif 1 (RRM1) found in the VICKZ
                     family proteins; cd12358"
                     /db_xref="CDD:240804"
     Region          103..178
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          207..274
                     /region_name="KH-I_IGF2BP_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22400"
                     /db_xref="CDD:411828"
     Site            order(215,217..219,221..225,228..229,232..233,238..241)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411828"
     Region          288..358
                     /region_name="KH-I_IGF2BP_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22401"
                     /db_xref="CDD:411829"
     Site            order(295,297..299,301..305,308..309,312..313,318..321)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411829"
     Region          424..493
                     /region_name="KH-I_IGF2BP_rpt3"
                     /note="third type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22402"
                     /db_xref="CDD:411830"
     Site            order(435..437,439..443,446..447,457..461,474)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411830"
     Region          511..578
                     /region_name="KH-I_IGF2BP_rpt4"
                     /note="fourth type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22403"
                     /db_xref="CDD:411831"
     Site            order(522..523,525..529,532,543..549)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411831"
     CDS             1..644
                     /gene="Imp"
                     /coded_by="XM_034327411.2:1232..3166"
                     /db_xref="GeneID:117605743"
ORIGIN      
        1 msklyvgnlp sdcnegalrq lfqdhnlsct tilvkrggya fvdctdqsva draidklngy
       61 tylgsalvve psvasgpkkr gsgdalpess lgqvgsgwsg agskvlvsnf paharledie
      121 ilfsncgqvq sveklssrdp ntqtvlvsye tqeqaqqavn qfngheyegn plkvemstve
      181 srrrgrsqrs gvtysgvsgs grqtdfplri lvqsdmvgai igrqgstirq itqmtrarvd
      241 vhrkdnvgsl ekaitiygnp enctnackki levmqqeans inkgeitlki lahnnligri
      301 igkggttikr imqdtdtkit vssindinsf nleriitvkg tienmskaes missklrqsy
      361 endlqamapq smmfpglhpm ammstagmgy ssrgpglygs gpapypyqas lppqqgvpat
      421 dtqetaflyi pnssvgaiig tkgshirnii rfsgasvkia pieqdkpadq qaerkvtivg
      481 spesqwkaqy lifekmreeg fvagtedvrl tieilvpsaq vgriigkggq nvrelqrvtg
      541 sviklseqqs tspsadeeat vhiigpffsv qsaqrrirsm vlqsgapgag gagsragrgs
      601 sqeggsrsrr dgsttsqqgg attqqhssqq sssspssqqq pqnq
//
LOCUS       XP_034183303             625 aa            linear   INV 03-SEP-2025
DEFINITION  IGF-II mRNA-binding protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183303
VERSION     XP_034183303.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327412.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..625
                     /product="IGF-II mRNA-binding protein isoform X2"
                     /calculated_mol_wt=67337
     Region          4..79
                     /region_name="RRM1_VICKZ"
                     /note="RNA recognition motif 1 (RRM1) found in the VICKZ
                     family proteins; cd12358"
                     /db_xref="CDD:240804"
     Region          84..159
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          188..255
                     /region_name="KH-I_IGF2BP_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22400"
                     /db_xref="CDD:411828"
     Site            order(196,198..200,202..206,209..210,213..214,219..222)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411828"
     Region          269..339
                     /region_name="KH-I_IGF2BP_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22401"
                     /db_xref="CDD:411829"
     Site            order(276,278..280,282..286,289..290,293..294,299..302)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411829"
     Region          405..474
                     /region_name="KH-I_IGF2BP_rpt3"
                     /note="third type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22402"
                     /db_xref="CDD:411830"
     Site            order(416..418,420..424,427..428,438..442,455)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411830"
     Region          492..559
                     /region_name="KH-I_IGF2BP_rpt4"
                     /note="fourth type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22403"
                     /db_xref="CDD:411831"
     Site            order(503..504,506..510,513,524..530)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411831"
     CDS             1..625
                     /gene="Imp"
                     /coded_by="XM_034327412.2:1230..3107"
                     /db_xref="GeneID:117605743"
ORIGIN      
        1 msklyvgnlp sdcnegalrq lfqdhnlsct tilvkrggya fvdctdqsva draidklngy
       61 tylgsalvve psvasgpkkr gagskvlvsn fpaharledi eilfsncgqv qsveklssrd
      121 pntqtvlvsy etqeqaqqav nqfngheyeg nplkvemstv esrrrgrsqr sgvtysgvsg
      181 sgrqtdfplr ilvqsdmvga iigrqgstir qitqmtrarv dvhrkdnvgs lekaitiygn
      241 penctnackk ilevmqqean sinkgeitlk ilahnnligr iigkggttik rimqdtdtki
      301 tvssindins fnleriitvk gtienmskae smissklrqs yendlqamap qsmmfpglhp
      361 mammstagmg yssrgpglyg sgpapypyqa slppqqgvpa tdtqetafly ipnssvgaii
      421 gtkgshirni irfsgasvki apieqdkpad qqaerkvtiv gspesqwkaq ylifekmree
      481 gfvagtedvr ltieilvpsa qvgriigkgg qnvrelqrvt gsviklseqq stspsadeea
      541 tvhiigpffs vqsaqrrirs mvlqsgapga ggagsragrg ssqeggsrsr rdgsttsqqg
      601 gattqqhssq qsssspssqq qpqnq
//
LOCUS       XP_034183305             575 aa            linear   INV 03-SEP-2025
DEFINITION  IGF-II mRNA-binding protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034183305
VERSION     XP_034183305.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327414.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..575
                     /product="IGF-II mRNA-binding protein isoform X3"
                     /calculated_mol_wt=62002
     Region          34..109
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     Region          138..205
                     /region_name="KH-I_IGF2BP_rpt1"
                     /note="first type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22400"
                     /db_xref="CDD:411828"
     Site            order(146,148..150,152..156,159..160,163..164,169..172)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411828"
     Region          219..289
                     /region_name="KH-I_IGF2BP_rpt2"
                     /note="second type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22401"
                     /db_xref="CDD:411829"
     Site            order(226,228..230,232..236,239..240,243..244,249..252)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:411829"
     Region          355..424
                     /region_name="KH-I_IGF2BP_rpt3"
                     /note="third type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22402"
                     /db_xref="CDD:411830"
     Site            order(366..368,370..374,377..378,388..392,405)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411830"
     Region          442..509
                     /region_name="KH-I_IGF2BP_rpt4"
                     /note="fourth type I K homology (KH) RNA-binding domain
                     found in the insulin-like growth factor 2 mRNA-binding
                     protein (IGF2BP) family; cd22403"
                     /db_xref="CDD:411831"
     Site            order(453..454,456..460,463,474..480)
                     /site_type="other"
                     /note="RNA binding site [nucleotide binding]"
                     /db_xref="CDD:411831"
     CDS             1..575
                     /gene="Imp"
                     /coded_by="XM_034327414.2:1686..3413"
                     /db_xref="GeneID:117605743"
ORIGIN      
        1 msldkfaegg mlskemerld ieekngdtas gagskvlvsn fpaharledi eilfsncgqv
       61 qsveklssrd pntqtvlvsy etqeqaqqav nqfngheyeg nplkvemstv esrrrgrsqr
      121 sgvtysgvsg sgrqtdfplr ilvqsdmvga iigrqgstir qitqmtrarv dvhrkdnvgs
      181 lekaitiygn penctnackk ilevmqqean sinkgeitlk ilahnnligr iigkggttik
      241 rimqdtdtki tvssindins fnleriitvk gtienmskae smissklrqs yendlqamap
      301 qsmmfpglhp mammstagmg yssrgpglyg sgpapypyqa slppqqgvpa tdtqetafly
      361 ipnssvgaii gtkgshirni irfsgasvki apieqdkpad qqaerkvtiv gspesqwkaq
      421 ylifekmree gfvagtedvr ltieilvpsa qvgriigkgg qnvrelqrvt gsviklseqq
      481 stspsadeea tvhiigpffs vqsaqrrirs mvlqsgapga ggagsragrg ssqeggsrsr
      541 rdgsttsqqg gattqqhssq qsssspssqq qpqnq
//
LOCUS       XP_076548202             580 aa            linear   INV 03-SEP-2025
DEFINITION  carboxylic ester hydrolase [Osmia lignaria lignaria].
ACCESSION   XP_076548202
VERSION     XP_076548202.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692087.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..580
                     /product="carboxylic ester hydrolase"
                     /calculated_mol_wt=65356
     Region          26..559
                     /region_name="COesterase"
                     /note="Carboxylesterase family; pfam00135"
                     /db_xref="CDD:395084"
     Site            order(136..138,216..218,221,374,379..380,419,472,475)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238191"
     Site            order(217,347,471)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:238191"
     CDS             1..580
                     /gene="LOC117605746"
                     /coded_by="XM_076692087.1:493..2235"
                     /db_xref="GeneID:117605746"
ORIGIN      
        1 mgyhrllwll lnciclfelv tikahtpmvt ikngtlsgmi mttrrgrefl gfrgipyalp
       61 plgelrfepp kpasswngvq saqedaqicv qrniythqde iegdedclyl nvytpklpnk
      121 eenlkggypv miwfhgcgwi cgaghsgfyh pkflldhdmi lvtvnyrlgp lgflstedtv
      181 cpgnnglkdq aqsirwvhen iaafggdpnr vtifgesagg asvhyhmmsn lskglfhrai
      241 sqsgnghcpw alakpgspkk kalklaeild cpsdskqlla clrkknavdi iatdrafqvf
      301 gycpmipfrp viepvhpgaf ltedpaissk hgrlsdipwm tgitseegsl ivpglytrnn
      361 gelikrlnnd flniapitll fgetcpkdeq krvaseiref yfgkapidns trfklidmys
      421 dawfihaaqt svinylekqs spvyyyylay rasasfsmif gdpnndygvs haddlqylfp
      481 vgeelfkdip lskqdykmid litsfwynfa ssgnptpevt eevpvkwkpv rtkdleylri
      541 gnngiemskn lnpdrmsfwe rlpirpdlde grtqqqkdel
//
LOCUS       XP_034183317             295 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605750 [Osmia lignaria lignaria].
ACCESSION   XP_034183317
VERSION     XP_034183317.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327426.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..295
                     /product="uncharacterized protein LOC117605750"
                     /calculated_mol_wt=33760
     Region          98..>209
                     /region_name="yhbS"
                     /note="Predicted N-acetyltransferase YhbS [General
                     function prediction only]; COG3153"
                     /db_xref="CDD:442387"
     Region          161..200
                     /region_name="NAT_SF"
                     /note="N-Acyltransferase superfamily: Various enzymes that
                     characteristically catalyze the transfer of an acyl group
                     to a substrate; cd04301"
                     /db_xref="CDD:173926"
     Site            order(178..180,190..191)
                     /site_type="other"
                     /note="Coenzyme A binding pocket [chemical binding]"
                     /db_xref="CDD:173926"
     CDS             1..295
                     /gene="LOC117605750"
                     /coded_by="XM_034327426.2:179..1066"
                     /db_xref="GeneID:117605750"
ORIGIN      
        1 mnnsnhntgr kkssikhsdl fvlsqqivdi rrqrlyqnyr sadatvvwer ishsyrivdl
       61 qldrfiqslr fvlvhycqte plaqvvglyd dgvsmrsllh qifdkmsenl sfcaledgtc
      121 elvgvlvasm fdktrckfal eypgdglrqi timrdyaitk amfyeimgae kpvridilcv
      181 rsdhqrkgia tallrsclar isgfasvcvg eftsgasqti aqrlqfevlc elpyenmeae
      241 ghryqifkdc ypenysiacm avkilppppr yqpsiksvsl htqkpkkrkk trkkk
//
LOCUS       XP_034183318             243 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605751 [Osmia lignaria lignaria].
ACCESSION   XP_034183318
VERSION     XP_034183318.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327427.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183318.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..243
                     /product="uncharacterized protein LOC117605751"
                     /calculated_mol_wt=27616
     CDS             1..243
                     /gene="LOC117605751"
                     /coded_by="XM_034327427.2:111..842"
                     /db_xref="GeneID:117605751"
ORIGIN      
        1 mdfneddrav emearmlapl ewestancki idlreplyne vlrlikrhff reepmckass
       61 flqdqpsvke ylelvhtwik dttsliatsl isgrvvgvav mridneadkt ntynrvqdlh
      121 geslkkimhl rntliqqtnp hkqfaqkeyv tiyilyvhps yqqrgveval lngcvqaava
      181 lkmpaiggif tcgmsqskaq svgfqllsei rysqwivndr vifddpgrgn ysaafmgkti
      241 plk
//
LOCUS       XP_034183312             395 aa            linear   INV 03-SEP-2025
DEFINITION  nuclear receptor subfamily 2 group E member 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183312
VERSION     XP_034183312.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327421.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..395
                     /product="nuclear receptor subfamily 2 group E member 1"
                     /calculated_mol_wt=44298
     Region          11..91
                     /region_name="NR_DBD_PNR_like_1"
                     /note="DNA-binding domain of the photoreceptor
                     cell-specific nuclear receptor (PNR) like proteins is
                     composed of two C4-type zinc fingers; cd07164"
                     /db_xref="CDD:143538"
     Site            order(11,14,28,31,50,56,66,69)
                     /site_type="other"
                     /note="zinc binding site [ion binding]"
                     /db_xref="CDD:143538"
     Site            order(21..23,29..30,32,34,37,40,63..64,67,70,81,84)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143538"
     Region          190..378
                     /region_name="NR_LBD"
                     /note="The ligand binding domain of nuclear receptors, a
                     family of ligand-activated transcription regulators;
                     cl11397"
                     /db_xref="CDD:472173"
     Site            order(214..215,218,221..222,251..252,255..256,259,262,291)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:132726"
     Site            order(223,226,230,235,240..241,243..244,247..248)
                     /site_type="active"
                     /note="coregulator recognition site [active]"
                     /db_xref="CDD:132726"
     CDS             1..395
                     /gene="LOC117605748"
                     /coded_by="XM_034327421.2:192..1379"
                     /db_xref="GeneID:117605748"
ORIGIN      
        1 mednsrgesl ckvcgdkasg khygvpscdg crgffkrsir ryarnldyvc kengrcivdv
       61 srrnqcqacr ftkclqvnmk rdavqherap rntsslvtaa rrgpsglypg iphvyhaasn
      121 pyhpllypal fpfkptvsaf tpsvahflpr sptesltvtp akedevtsse eagtidtrie
      181 pkeeltssrl apptinsvss eyvpnfsilp teniyefaak llffavrwar sihsflqlpy
      241 rdqtillees wselfvltaa qwnfpveetv lvpndilper kevlvdevrr lrellakctl
      301 lrvdhseyac lkaivlfkge srglcepgrv talqeqtvav fcerdarrvg rlllllppar
      361 alcrstlqel lfkptvgdvs verllgdmvs alrpt
//
LOCUS       XP_034194159             508 aa            linear   INV 03-SEP-2025
DEFINITION  amine oxidase [flavin-containing] A-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034194159
VERSION     XP_034194159.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338268.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034194159.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..508
                     /product="amine oxidase [flavin-containing] A-like isoform
                     X1"
                     /calculated_mol_wt=58172
     Region          15..451
                     /region_name="YobN"
                     /note="Monoamine oxidase [Amino acid transport and
                     metabolism]; COG1231"
                     /db_xref="CDD:440844"
     CDS             1..508
                     /gene="LOC117610642"
                     /coded_by="XM_034338268.2:134..1660"
                     /db_xref="GeneID:117610642"
ORIGIN      
        1 maadfadeef vredandfdv iivgagltgl tcaynilqkk tglsvliiee nnevggrilp
       61 kafsetyhan flqehitdli ktmdlnidrr kiidmrkril ctnyrpsqnl pkyygaevys
      121 flqmmekeai alklstntmh neiehlaets veellrkivy fsfarslcra yicsacamrn
      181 lndisvlwll svlngalglf nrlrimfgdn nryfiqggvt kiahkllkni lrfygkirya
      241 evvneikfnd drvyvltekn hfrcelvvia vpppmhnrii ikpssqclln tqilyipskn
      301 vffnvayknl hwndnsikdv vttwdsnsnl nivydvtrsn serfmlsgfl aepdstqtnr
      361 kglfntlnqc fetidtskyl qykeynhfsn dqmkggspms vmmptsinsy inymgttfer
      421 iffasseyat nwpgtidgai evgkmtaysi lyrirpqvlt sheiaslkfe garqiepvpf
      481 kicaiqylgl mlnvtvflsf mktayist
//
LOCUS       XP_076548203             426 aa            linear   INV 03-SEP-2025
DEFINITION  amine oxidase [flavin-containing] A-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548203
VERSION     XP_076548203.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692088.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="amine oxidase [flavin-containing] A-like isoform
                     X2"
                     /calculated_mol_wt=49131
     Region          <61..369
                     /region_name="YobN"
                     /note="Monoamine oxidase [Amino acid transport and
                     metabolism]; COG1231"
                     /db_xref="CDD:440844"
     CDS             1..426
                     /gene="LOC117610642"
                     /coded_by="XM_076692088.1:231..1511"
                     /db_xref="GeneID:117610642"
ORIGIN      
        1 mdlnidrrki idmrkrilct nyrpsqnlpk yygaevysfl qmmekeaial klstntmhne
       61 iehlaetsve ellrkivyfs farslcrayi csacamrnln disvlwllsv lngalglfnr
      121 lrimfgdnnr yfiqggvtki ahkllknilr fygkiryaev vneikfnddr vyvlteknhf
      181 rcelvviavp ppmhnriiik pssqcllntq ilyipsknvf fnvayknlhw ndnsikdvvt
      241 twdsnsnlni vydvtrsnse rfmlsgflae pdstqtnrkg lfntlnqcfe tidtskylqy
      301 keynhfsndq mkggspmsvm mptsinsyin ymgttferif fasseyatnw pgtidgaiev
      361 gkmtaysily rirpqvltsh eiaslkfega rqiepvpfki caiqylglml nvtvflsfmk
      421 tayist
//
LOCUS       XP_076548204             395 aa            linear   INV 03-SEP-2025
DEFINITION  amine oxidase [flavin-containing] A-like isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548204
VERSION     XP_076548204.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692089.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..395
                     /product="amine oxidase [flavin-containing] A-like isoform
                     X3"
                     /calculated_mol_wt=45349
     Region          <30..338
                     /region_name="YobN"
                     /note="Monoamine oxidase [Amino acid transport and
                     metabolism]; COG1231"
                     /db_xref="CDD:440844"
     CDS             1..395
                     /gene="LOC117610642"
                     /coded_by="XM_076692089.1:140..1327"
                     /db_xref="GeneID:117610642"
ORIGIN      
        1 mciqysaken wmekeaialk lstntmhnei ehlaetsvee llrkivyfsf arslcrayic
       61 sacamrnlnd isvlwllsvl ngalglfnrl rimfgdnnry fiqggvtkia hkllknilrf
      121 ygkiryaevv neikfnddrv yvlteknhfr celvviavpp pmhnriiikp ssqcllntqi
      181 lyipsknvff nvayknlhwn dnsikdvvtt wdsnsnlniv ydvtrsnser fmlsgflaep
      241 dstqtnrkgl fntlnqcfet idtskylqyk eynhfsndqm kggspmsvmm ptsinsyiny
      301 mgttferiff asseyatnwp gtidgaievg kmtaysilyr irpqvltshe iaslkfegar
      361 qiepvpfkic aiqylglmln vtvflsfmkt ayist
//
LOCUS       XP_034194168             858 aa            linear   INV 03-SEP-2025
DEFINITION  P protein [Osmia lignaria lignaria].
ACCESSION   XP_034194168
VERSION     XP_034194168.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034338277.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..858
                     /product="P protein"
                     /calculated_mol_wt=96542
     Region          363..848
                     /region_name="P_permease"
                     /note="Permease P (pink-eyed dilution). Mutations in the
                     human melanosomal P gene were responsible for classic
                     phenotype of oculocutaneous albinism type 2 (OCA2).
                     Although the precise function of the P protein is unknown,
                     it was predicted to regulate the...; cd01116"
                     /db_xref="CDD:238536"
     Site            order(365..368,375..388,408..425,448..464,466..483,
                     488..511,538..552,643..655,670..684,696..714,743..758,
                     788..807,833..845)
                     /site_type="other"
                     /note="transmembrane helices"
                     /db_xref="CDD:238536"
     CDS             1..858
                     /gene="LOC117610651"
                     /coded_by="XM_034338277.1:29..2605"
                     /db_xref="GeneID:117610651"
ORIGIN      
        1 mclhkldltn idsfsynknv eishfriyif rnynlkikam qykmsqsess ddetglqtpm
       61 flrkgsrcsr gtpvtpssia ssvifgqipe evlrvwsqlp eairldpsla vfqreydrvh
      121 qteegrssdv vkkdclvvnm slgtqtalqq rirnkgsree dghdvtqeph evkqnpfyry
      181 iklilltccw liftliliik sekveamhqi svpkgeikny kmhgdisgre isvtiegsll
      241 ppiqskeytn vsdrylmvwl ellvnetnnt deffsvhskq iknisnvwml pilpenlmdl
      301 fsgqryhnif elqnvnkeml kgglasinlk tnldssfavs isydlssink ddgiayaaiv
      361 lfglyvlivf eivhrtlaam lastmsiail aalnerpsmv elvswidvdt lmllfammil
      421 vaviaetgif dwlavyayki tggelwplig tlcfftafis afldnvttvl lmtpvtirlc
      481 evmeinpvpi ltamvvysni ggamtpigdp pnviiasnrd vinngidfst ftmhmsigvi
      541 lviivayvql rfifrdvdvl rfdeppdvqe lrheiaiwqr aaaslssysk denlvretll
      601 kkvqrlvsql krkivtgsvt lerykttlee lqekypirdk wllvksgfal ifvitlfflh
      661 svpnlhlslg ltalvgvlll liladsedld glmarvewst llffaslfvl mealsrlgli
      721 awigkqteri ilsvneesrl availlllwv stfasafvdn vplstmmiri vinlaqnrel
      781 rlplqplvwa lafggcmggn gtligatanv vcvgvaeqhg ykftfmqffk vgfpimltsa
      841 atitmylmia hvvfdwng
//
LOCUS       XP_034183038             619 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605618 [Osmia lignaria lignaria].
ACCESSION   XP_034183038
VERSION     XP_034183038.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327147.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183038.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..619
                     /product="uncharacterized protein LOC117605618"
                     /calculated_mol_wt=70302
     CDS             1..619
                     /gene="LOC117605618"
                     /coded_by="XM_034327147.2:265..2124"
                     /db_xref="GeneID:117605618"
ORIGIN      
        1 mtwftifatl livqsgisdv viygelrrsv klsastevlg eikkcvmetn igdkykfspg
       61 agsigdrnvt iianyldcsv llylpsaefa gewkitsyfq ednhppvtnk fkiivkketr
      121 vipdyiwint dyfvhaeldl ngknvtnctl ltptsqeidl lndgtknlqr fgrcgfralv
      181 ndksqniwkl kafveedkif ytggvdvnvf nkekkteeei titlnlgtsg tvslpvyrti
      241 ifcqledpmg lktpfldscd yhipvvtprh qgvwiarygg mehpvptvqr frvvtrdpvn
      301 sdsnvivten neiqlschiy dqvpmsfrfc hfvrpdeyvl nlrngigteg yssfntfean
      361 niycgliihe pkqhdygawk cnvklqdrky rgsiiyvdpr imstnetqna trefhivten
      421 vyvkrndffe ikctvdavld ycwfrspngt fytvipnrrq sptnllytgs gfnfgecvag
      481 idsasytdhg ewtcnvgivd gpeesktvlv nvtesyvipe rtelvanyee dtilscnilp
      541 nitdkniryc rwvrpdghgi hhslgprytt nrsntgcklm iadcdtekdi grwtcvfgly
      601 gfedeeasat isvllpthr
//
LOCUS       XP_034187082             322 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607467 [Osmia lignaria lignaria].
ACCESSION   XP_034187082
VERSION     XP_034187082.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..322
                     /product="uncharacterized protein LOC117607467"
                     /calculated_mol_wt=36513
     Region          257..320
                     /region_name="bHLH_TS_amos_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Drosophila melanogaster protein Amos and similar proteins;
                     cd19715"
                     /db_xref="CDD:381558"
     Site            order(265,269,271..273,275,280,298..301)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381558"
     Site            order(278..279,282..283,285..286,302,305,309,311..312,316,
                     318..319)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381558"
     CDS             1..322
                     /gene="LOC117607467"
                     /coded_by="XM_034331191.2:279..1247"
                     /db_xref="GeneID:117607467"
ORIGIN      
        1 mtaytsfqqy dlldsegygs asspesnprs wthpeiypqp prsrgsscgs vssidcqnre
       61 yqeigatngs fhvtatgfnf tefyegyyqe gcrndhqish pnnprivkeh gkmyrmnecv
      121 enydgvpgrt efsnevpsvr qnpdvstkve hnvfgnsrcd fvqnqenffp sksygvpkgd
      181 gflarnngnp ygqrsdvlyl gaaysvhrne nyiqnnnqsg kcepsryqkn dalhvssmey
      241 ggqkkdamqk ikngtpgiev lrkrrlaana rerrrmnsln dafdrlrdvv pslgndrkls
      301 kfetlqmaqt yiaalyellq re
//
LOCUS       XP_034191257            3903 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609264 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191257
VERSION     XP_034191257.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335366.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191257.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3903
                     /product="uncharacterized protein LOC117609264 isoform X1"
                     /calculated_mol_wt=435794
     Region          <245..309
                     /region_name="DNA_pol_phi"
                     /note="DNA polymerase phi; pfam04931"
                     /db_xref="CDD:461488"
     Region          1293..>2192
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          2642..2687
                     /region_name="PHD_RSF1"
                     /note="PHD finger found in Remodeling and spacing factor 1
                     (Rsf-1); cd15543"
                     /db_xref="CDD:277018"
     Site            order(2642,2654..2658,2662,2682)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277018"
     Region          3550..3777
                     /region_name="MISS"
                     /note="MAPK-interacting and spindle-stabilising
                     protein-like; pfam15822"
                     /db_xref="CDD:318115"
     CDS             1..3903
                     /gene="LOC117609264"
                     /coded_by="XM_034335366.2:478..12189"
                     /db_xref="GeneID:117609264"
ORIGIN      
        1 masdneasca sdpnfavics flecfgkscg iiypdiarlq emlentqevp qelidlhikl
       61 lrktrktvsp ekweralvkf chtysnqdgw elerfgykka riavklrllk vlletqfdln
      121 qkfknevnkl aanelrvepl grdksglayw cqldeecnir vyredldeen welvakdrdg
      181 vvnlintlsn geigdipine dsnsleisek piidtgqitt spsledeslq engihaedlc
      241 dkqsnkhege dsedeqeqde fnegdniedd idgedeitne ddsskqntee ddsqetvqse
      301 ilesnlikaf plmtasskpl ngkinvlsen etvtsrkkki dtvpetagtv sdyksmdtck
      361 skehievnnv kvksgesvpl ksietsvits plklvnvset kqvdeekias gvtalnitkk
      421 lhnedssdqi lkpldklsgk sfsfssteih tahsklsvkp idqlaanlvr mqseklekpi
      481 gakslekiae nlarsgsivg nvingdddrl pqdfcarsqm ekanvharen rgmdlstspr
      541 gwestseqnk pmdfsgidls srkfnkpmdi lssgyrtqdf qhremdlstk klnkpevtsl
      601 pydaravmmr nhamvadlsk rqmsfpsydm ssvspyhnaa ripnkddhrl pnytilpdps
      661 klaalrmntt pvkrsleadd lqqdmlkrir advipiqrsi dkrpmigatw rdeigeavee
      721 pvmmiqgegs gsdcdavnpg lgeaveepii ffygegsgee cqtgnpgdst sadnkesnes
      781 kneadsatss lstnksfseg slinevsaes lvtnksaikl nrnypqsnin vksnfqivgs
      841 tqekpkfkpt lgiqiipknt nssvkrlsrw dvgkpeekpe cdssiisnee nisqrrnkie
      901 cedsssenqd vkidgnsisi edvtsqqsnv klenlqenig esssaieekq sstdqdsiqc
      961 cdssislcqa dtsetfksct ktksvtendk tniknvcdie stslhetsts ermdneckps
     1021 avspprfffg pncisytskt emfesqpdht vstsiietnk sdtlqyecvs ssrpaedtin
     1081 tyksddfdvt qtnnnslkvn lyphktanpv ceddnknkie kiegaanswe psasskysle
     1141 qpsklrktei slhdpesvve ieedstscti dtvqdsisst eaaskecikf ketemldtis
     1201 qphstndlin kkdvdmkmen avvipselel kqecnifeeq nkeesdsadd ivkslntsip
     1261 cseaepycks sefkenklea dsvssksscs ssdgiqnfqk nnftesedht setecfvyqe
     1321 tndlaekeld yisqktfdss vticeennen rddrkyenqn eeliefdnki ttdligsdae
     1381 avlvncnesn eeinrqnevl edsenegkkv fidpfiknfv pdvntkldsi dnstvtssef
     1441 iekcnkdtlv ptisssddsc ihqdtsfhes vklnsekssf kdecptydtl lpetvpdtni
     1501 snssrsnivd stkesnvvqa aiskgqssci dqdlnnlskd eegstklyeg eacsnenlss
     1561 nenayndslk lqspsletnd sgiisekdkt gnvnfveiqe kfdtviepnk tddrtqfcke
     1621 dvteyvqdsy qedqkqaivs niletnskta ekseieqeel khtkinpqel inseikqslv
     1681 anyessdfdd snddvfeafe tepiesksnf ssdleeivsd aeeneeknie kpskkneils
     1741 nevqgipvdk etesmidssg iekitvdkse ekekglneet dfnvvqhdln leialdekir
     1801 lvnseqnssn tvelaetets tisseinpik kecislelnk annliniike gkkessdevr
     1861 knlvdvvvpv ektdfkidat neietvelln nsnneiiped lrtelslkst eenlvtsldn
     1921 dvkfmkneev khvteeisii etcnkvrkss ehgdkenkfd inlqeeknfn keecnvsihs
     1981 vsnilplkfd niekseihkn nisvmsenfk pmdnlqyvsf mseksensdi idnhenrnks
     2041 lrvtnetide sisestvtsv kdsstsfstq qgmitsapii aindykkeas krfsdvsdli
     2101 qdvpplkskp pciepaneei lqdlsnskaq lnrnldnfke melavnetka rddmitvsdn
     2161 iavqstkdec hrtvanvlnn ilnqierkni etneekestd iqsveiktvs lisddslgad
     2221 nenlfgiase emdplactdd dddddddddd ddndddddgt vkklddtpki alrvkpvsel
     2281 vydgwkldst eppklsrkrr nsshesnsed ggmkedevim mggkrmklrg krmadkqlrk
     2341 sveesrvvti ssedeavkcd dsqelkeqes kdasddqsga hlsaidrksr grprgrrrrg
     2401 ykgsgrsnrs khsefqssqv setspevife gtppsnndsl nmtpvsqkkr kkrkmvlgle
     2461 vgrdivpepe agltqdetpv rqsrriaqlk ikeaqlkiee eslsekkerk dkkekkdste
     2521 kkkrkkqkpe seeeiiikdi ekekkskkkr rkrkkkkmls kfneanpwqs ssgsssdeen
     2581 endeeeeeee diesegsllf ksdhefspes dlekdqesep lrrartaqka qsdveeadde
     2641 yacqkcgkad hpewillcds cdkgwhcscl rpalmlipeg dwfcppcqhn llvvklrenl
     2701 ktydqltkrh enevlrkkrl afvgisldnv lhknetqrhq kesnassqes dnesttttst
     2761 sstetsssee sepvyqlrer rcanrykfne ydaiinaaiq deveavqgag nqgrgkdiat
     2821 ivnakkeeae lefkneilnm deipankddi erksekdsde eykidaedgv deeeeeqhkg
     2881 tvkkflsrkk hrklnsldis seddpesded fkgtsseeee dfddhitssd dssfadmkrr
     2941 grkdsrsvrr strvrtmrye edfinddsee sdrpkrkksr stwdsdneds dnswrqrkkk
     3001 sktiptskyk tlpktksrkk kkrkriiesd nqsdnddste eqkietscvi qaqlennesv
     3061 tldalnmkve nvnennenkn vnemndikle emktevadis vtlplqdall qkkkkenatk
     3121 pkktptirrk iiygglpden kpeeeetlgr rtrgrkmnyq eeiasdseee lkkalrrtge
     3181 sedefvvneg edliedaekd sdsgdiynpk kdghkvknks pksrkarksk spggkrkmln
     3241 dgtpkprkkp gpkpgsknks rkqnflmgsm iqrrddqdee kdimgnvidn piggldskid
     3301 dnlsenvglt stamsvagsf tgdvpegslt glgpgeladl ddeqleqmmm edeeygrrql
     3361 elaaieiakk kkkeereakk lekarlkale ilaaerqrdp napegtdgev pkkkkrgrrs
     3421 kaeilaeqmr rdgapdlnat sltssvahtv asnisptvnl alnpavptim tpdaerpseg
     3481 pismmtgpdg qlfnpdgspl kpkrrgrgkg kktlaleaar aaeaaakaav evgllsmgtd
     3541 snpelkpgei pnilptpgss tsgsapstpp agvvttqgpp asqtpttqpv ypslppnqqs
     3601 svitrmlqsq pvssspqsfs aaaaamghky fggpntggqi mggprtgyem qprgripspy
     3661 rqagqssipp hfaavrsgtp pmrmrvpgpq iyhtphhpmd pspsgggpis insrdrsspl
     3721 gpgpamippa agsplakggp tppppppyvr gapplprftd npmgprhqmp pftnaspvnh
     3781 nmqqpspppn rppgnfspyh pppppnyhyg aypppppmst addaaayqgs pypadhfssp
     3841 adnqppiqap pppqpppppp qqqqahpnda agvgnkqyde egsgefgglv syfssqredd
     3901 lds
//
LOCUS       XP_034191259            3883 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609264 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191259
VERSION     XP_034191259.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335368.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191259.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3883
                     /product="uncharacterized protein LOC117609264 isoform X2"
                     /calculated_mol_wt=433755
     Region          <245..309
                     /region_name="DNA_pol_phi"
                     /note="DNA polymerase phi; pfam04931"
                     /db_xref="CDD:461488"
     Region          1293..>2192
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          2642..2687
                     /region_name="PHD_RSF1"
                     /note="PHD finger found in Remodeling and spacing factor 1
                     (Rsf-1); cd15543"
                     /db_xref="CDD:277018"
     Site            order(2642,2654..2658,2662,2682)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277018"
     Region          3530..3757
                     /region_name="MISS"
                     /note="MAPK-interacting and spindle-stabilising
                     protein-like; pfam15822"
                     /db_xref="CDD:318115"
     CDS             1..3883
                     /gene="LOC117609264"
                     /coded_by="XM_034335368.2:868..12519"
                     /db_xref="GeneID:117609264"
ORIGIN      
        1 masdneasca sdpnfavics flecfgkscg iiypdiarlq emlentqevp qelidlhikl
       61 lrktrktvsp ekweralvkf chtysnqdgw elerfgykka riavklrllk vlletqfdln
      121 qkfknevnkl aanelrvepl grdksglayw cqldeecnir vyredldeen welvakdrdg
      181 vvnlintlsn geigdipine dsnsleisek piidtgqitt spsledeslq engihaedlc
      241 dkqsnkhege dsedeqeqde fnegdniedd idgedeitne ddsskqntee ddsqetvqse
      301 ilesnlikaf plmtasskpl ngkinvlsen etvtsrkkki dtvpetagtv sdyksmdtck
      361 skehievnnv kvksgesvpl ksietsvits plklvnvset kqvdeekias gvtalnitkk
      421 lhnedssdqi lkpldklsgk sfsfssteih tahsklsvkp idqlaanlvr mqseklekpi
      481 gakslekiae nlarsgsivg nvingdddrl pqdfcarsqm ekanvharen rgmdlstspr
      541 gwestseqnk pmdfsgidls srkfnkpmdi lssgyrtqdf qhremdlstk klnkpevtsl
      601 pydaravmmr nhamvadlsk rqmsfpsydm ssvspyhnaa ripnkddhrl pnytilpdps
      661 klaalrmntt pvkrsleadd lqqdmlkrir advipiqrsi dkrpmigatw rdeigeavee
      721 pvmmiqgegs gsdcdavnpg lgeaveepii ffygegsgee cqtgnpgdst sadnkesnes
      781 kneadsatss lstnksfseg slinevsaes lvtnksaikl nrnypqsnin vksnfqivgs
      841 tqekpkfkpt lgiqiipknt nssvkrlsrw dvgkpeekpe cdssiisnee nisqrrnkie
      901 cedsssenqd vkidgnsisi edvtsqqsnv klenlqenig esssaieekq sstdqdsiqc
      961 cdssislcqa dtsetfksct ktksvtendk tniknvcdie stslhetsts ermdneckps
     1021 avspprfffg pncisytskt emfesqpdht vstsiietnk sdtlqyecvs ssrpaedtin
     1081 tyksddfdvt qtnnnslkvn lyphktanpv ceddnknkie kiegaanswe psasskysle
     1141 qpsklrktei slhdpesvve ieedstscti dtvqdsisst eaaskecikf ketemldtis
     1201 qphstndlin kkdvdmkmen avvipselel kqecnifeeq nkeesdsadd ivkslntsip
     1261 cseaepycks sefkenklea dsvssksscs ssdgiqnfqk nnftesedht setecfvyqe
     1321 tndlaekeld yisqktfdss vticeennen rddrkyenqn eeliefdnki ttdligsdae
     1381 avlvncnesn eeinrqnevl edsenegkkv fidpfiknfv pdvntkldsi dnstvtssef
     1441 iekcnkdtlv ptisssddsc ihqdtsfhes vklnsekssf kdecptydtl lpetvpdtni
     1501 snssrsnivd stkesnvvqa aiskgqssci dqdlnnlskd eegstklyeg eacsnenlss
     1561 nenayndslk lqspsletnd sgiisekdkt gnvnfveiqe kfdtviepnk tddrtqfcke
     1621 dvteyvqdsy qedqkqaivs niletnskta ekseieqeel khtkinpqel inseikqslv
     1681 anyessdfdd snddvfeafe tepiesksnf ssdleeivsd aeeneeknie kpskkneils
     1741 nevqgipvdk etesmidssg iekitvdkse ekekglneet dfnvvqhdln leialdekir
     1801 lvnseqnssn tvelaetets tisseinpik kecislelnk annliniike gkkessdevr
     1861 knlvdvvvpv ektdfkidat neietvelln nsnneiiped lrtelslkst eenlvtsldn
     1921 dvkfmkneev khvteeisii etcnkvrkss ehgdkenkfd inlqeeknfn keecnvsihs
     1981 vsnilplkfd niekseihkn nisvmsenfk pmdnlqyvsf mseksensdi idnhenrnks
     2041 lrvtnetide sisestvtsv kdsstsfstq qgmitsapii aindykkeas krfsdvsdli
     2101 qdvpplkskp pciepaneei lqdlsnskaq lnrnldnfke melavnetka rddmitvsdn
     2161 iavqstkdec hrtvanvlnn ilnqierkni etneekestd iqsveiktvs lisddslgad
     2221 nenlfgiase emdplactdd dddddddddd ddndddddgt vkklddtpki alrvkpvsel
     2281 vydgwkldst eppklsrkrr nsshesnsed ggmkedevim mggkrmklrg krmadkqlrk
     2341 sveesrvvti ssedeavkcd dsqelkeqes kdasddqsga hlsaidrksr grprgrrrrg
     2401 ykgsgrsnrs khsefqssqv setspevife gtppsnndsl nmtpvsqkkr kkrkmvlgle
     2461 vgrdivpepe agltqdetpv rqsrriaqlk ikeaqlkiee eslsekkerk dkkekkdste
     2521 kkkrkkqkpe seeeiiikdi ekekkskkkr rkrkkkkmls kfneanpwqs ssgsssdeen
     2581 endeeeeeee diesegsllf ksdhefspes dlekdqesep lrrartaqka qsdveeadde
     2641 yacqkcgkad hpewillcds cdkgwhcscl rpalmlipeg dwfcppcqhn llvvklrenl
     2701 ktydqltkrh enevlrkkrl afvgisldnv lhknetqrhq kesnassqes dnesttttst
     2761 sstetsssee sepvyqlrer rcanrykfne ydaiinaaiq deveavqgag nqgrgkdiat
     2821 ivnakkeeae lefkneilnm deipankddi erksekdsde eykidaedgv deeeeeqhkg
     2881 tvkkflsrkk hrklnsldis seddpesded fkgtsseeee dfddhitssd dssfadmkrr
     2941 grkdsrsvrr strvrtmrye edfinddsee sdrpkrkksr stwdsdneds dnswrqrkkk
     3001 sktiptskyk tlpktksrkk kkrkriiesd nqsdnddste eqkietscvi qaqlennesv
     3061 tldalnmkve nvnennenkn vnemndikle emktevadis vtlplqdall qkkkkenatk
     3121 pkktptirrk iiygglpden kpeeeetlgr rtrgrkmnyq eeiasdseee lkkalrrtge
     3181 sedefvvneg edliedaekd sdsgdiynpk kdghkvknks pksrkarksk spggkrkmln
     3241 dgtpkprkkp gpkpgsknks rkqnflmgsm iqrrddqdee kdimenvglt stamsvagsf
     3301 tgdvpegslt glgpgeladl ddeqleqmmm edeeygrrql elaaieiakk kkkeereakk
     3361 lekarlkale ilaaerqrdp napegtdgev pkkkkrgrrs kaeilaeqmr rdgapdlnat
     3421 sltssvahtv asnisptvnl alnpavptim tpdaerpseg pismmtgpdg qlfnpdgspl
     3481 kpkrrgrgkg kktlaleaar aaeaaakaav evgllsmgtd snpelkpgei pnilptpgss
     3541 tsgsapstpp agvvttqgpp asqtpttqpv ypslppnqqs svitrmlqsq pvssspqsfs
     3601 aaaaamghky fggpntggqi mggprtgyem qprgripspy rqagqssipp hfaavrsgtp
     3661 pmrmrvpgpq iyhtphhpmd pspsgggpis insrdrsspl gpgpamippa agsplakggp
     3721 tppppppyvr gapplprftd npmgprhqmp pftnaspvnh nmqqpspppn rppgnfspyh
     3781 pppppnyhyg aypppppmst addaaayqgs pypadhfssp adnqppiqap pppqpppppp
     3841 qqqqahpnda agvgnkqyde egsgefgglv syfssqredd lds
//
LOCUS       XP_076548206            3882 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609264 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548206
VERSION     XP_076548206.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692091.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3882
                     /product="uncharacterized protein LOC117609264 isoform X3"
                     /calculated_mol_wt=433654
     Region          <245..309
                     /region_name="DNA_pol_phi"
                     /note="DNA polymerase phi; pfam04931"
                     /db_xref="CDD:461488"
     Region          1293..>2192
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          2642..2687
                     /region_name="PHD_RSF1"
                     /note="PHD finger found in Remodeling and spacing factor 1
                     (Rsf-1); cd15543"
                     /db_xref="CDD:277018"
     Site            order(2642,2654..2658,2662,2682)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277018"
     Region          3529..3756
                     /region_name="MISS"
                     /note="MAPK-interacting and spindle-stabilising
                     protein-like; pfam15822"
                     /db_xref="CDD:318115"
     CDS             1..3882
                     /gene="LOC117609264"
                     /coded_by="XM_076692091.1:976..12624"
                     /db_xref="GeneID:117609264"
ORIGIN      
        1 masdneasca sdpnfavics flecfgkscg iiypdiarlq emlentqevp qelidlhikl
       61 lrktrktvsp ekweralvkf chtysnqdgw elerfgykka riavklrllk vlletqfdln
      121 qkfknevnkl aanelrvepl grdksglayw cqldeecnir vyredldeen welvakdrdg
      181 vvnlintlsn geigdipine dsnsleisek piidtgqitt spsledeslq engihaedlc
      241 dkqsnkhege dsedeqeqde fnegdniedd idgedeitne ddsskqntee ddsqetvqse
      301 ilesnlikaf plmtasskpl ngkinvlsen etvtsrkkki dtvpetagtv sdyksmdtck
      361 skehievnnv kvksgesvpl ksietsvits plklvnvset kqvdeekias gvtalnitkk
      421 lhnedssdqi lkpldklsgk sfsfssteih tahsklsvkp idqlaanlvr mqseklekpi
      481 gakslekiae nlarsgsivg nvingdddrl pqdfcarsqm ekanvharen rgmdlstspr
      541 gwestseqnk pmdfsgidls srkfnkpmdi lssgyrtqdf qhremdlstk klnkpevtsl
      601 pydaravmmr nhamvadlsk rqmsfpsydm ssvspyhnaa ripnkddhrl pnytilpdps
      661 klaalrmntt pvkrsleadd lqqdmlkrir advipiqrsi dkrpmigatw rdeigeavee
      721 pvmmiqgegs gsdcdavnpg lgeaveepii ffygegsgee cqtgnpgdst sadnkesnes
      781 kneadsatss lstnksfseg slinevsaes lvtnksaikl nrnypqsnin vksnfqivgs
      841 tqekpkfkpt lgiqiipknt nssvkrlsrw dvgkpeekpe cdssiisnee nisqrrnkie
      901 cedsssenqd vkidgnsisi edvtsqqsnv klenlqenig esssaieekq sstdqdsiqc
      961 cdssislcqa dtsetfksct ktksvtendk tniknvcdie stslhetsts ermdneckps
     1021 avspprfffg pncisytskt emfesqpdht vstsiietnk sdtlqyecvs ssrpaedtin
     1081 tyksddfdvt qtnnnslkvn lyphktanpv ceddnknkie kiegaanswe psasskysle
     1141 qpsklrktei slhdpesvve ieedstscti dtvqdsisst eaaskecikf ketemldtis
     1201 qphstndlin kkdvdmkmen avvipselel kqecnifeeq nkeesdsadd ivkslntsip
     1261 cseaepycks sefkenklea dsvssksscs ssdgiqnfqk nnftesedht setecfvyqe
     1321 tndlaekeld yisqktfdss vticeennen rddrkyenqn eeliefdnki ttdligsdae
     1381 avlvncnesn eeinrqnevl edsenegkkv fidpfiknfv pdvntkldsi dnstvtssef
     1441 iekcnkdtlv ptisssddsc ihqdtsfhes vklnsekssf kdecptydtl lpetvpdtni
     1501 snssrsnivd stkesnvvqa aiskgqssci dqdlnnlskd eegstklyeg eacsnenlss
     1561 nenayndslk lqspsletnd sgiisekdkt gnvnfveiqe kfdtviepnk tddrtqfcke
     1621 dvteyvqdsy qedqkqaivs niletnskta ekseieqeel khtkinpqel inseikqslv
     1681 anyessdfdd snddvfeafe tepiesksnf ssdleeivsd aeeneeknie kpskkneils
     1741 nevqgipvdk etesmidssg iekitvdkse ekekglneet dfnvvqhdln leialdekir
     1801 lvnseqnssn tvelaetets tisseinpik kecislelnk annliniike gkkessdevr
     1861 knlvdvvvpv ektdfkidat neietvelln nsnneiiped lrtelslkst eenlvtsldn
     1921 dvkfmkneev khvteeisii etcnkvrkss ehgdkenkfd inlqeeknfn keecnvsihs
     1981 vsnilplkfd niekseihkn nisvmsenfk pmdnlqyvsf mseksensdi idnhenrnks
     2041 lrvtnetide sisestvtsv kdsstsfstq qgmitsapii aindykkeas krfsdvsdli
     2101 qdvpplkskp pciepaneei lqdlsnskaq lnrnldnfke melavnetka rddmitvsdn
     2161 iavqstkdec hrtvanvlnn ilnqierkni etneekestd iqsveiktvs lisddslgad
     2221 nenlfgiase emdplactdd dddddddddd ddndddddgt vkklddtpki alrvkpvsel
     2281 vydgwkldst eppklsrkrr nsshesnsed ggmkedevim mggkrmklrg krmadkqlrk
     2341 sveesrvvti ssedeavkcd dsqelkeqes kdasddqsga hlsaidrksr grprgrrrrg
     2401 ykgsgrsnrs khsefqssqv setspevife gtppsnndsl nmtpvsqkkr kkrkmvlgle
     2461 vgrdivpepe agltqdetpv rqsrriaqlk ikeaqlkiee eslsekkerk dkkekkdste
     2521 kkkrkkqkpe seeeiiikdi ekekkskkkr rkrkkkkmls kfneanpwqs ssgsssdeen
     2581 endeeeeeee diesegsllf ksdhefspes dlekdqesep lrrartaqka qsdveeadde
     2641 yacqkcgkad hpewillcds cdkgwhcscl rpalmlipeg dwfcppcqhn llvvklrenl
     2701 ktydqltkrh enevlrkkrl afvgisldnv lhknetqrhq kesnassqes dnesttttst
     2761 sstetsssee sepvyqlrer rcanrykfne ydaiinaaiq deveavqgag nqgrgkdiat
     2821 ivnakkeeae lefkneilnm deipankddi erksekdsde eykidaedgv deeeeeqhkg
     2881 tvkkflsrkk hrklnsldis seddpesded fkgtsseeee dfddhitssd dssfadmkrr
     2941 grkdsrsvrr strvrtmrye edfinddsee sdrpkrkksr stwdsdneds dnswrqrkkn
     3001 ktiptskykt lpktksrkkk krkriiesdn qsdnddstee qkietscviq aqlennesvt
     3061 ldalnmkven vnennenknv nemndiklee mktevadisv tlplqdallq kkkkenatkp
     3121 kktptirrki iygglpdenk peeeetlgrr trgrkmnyqe eiasdseeel kkalrrtges
     3181 edefvvnege dliedaekds dsgdiynpkk dghkvknksp ksrkarksks pggkrkmlnd
     3241 gtpkprkkpg pkpgsknksr kqnflmgsmi qrrddqdeek dimenvglts tamsvagsft
     3301 gdvpegsltg lgpgeladld deqleqmmme deeygrrqle laaieiakkk kkeereakkl
     3361 ekarlkalei laaerqrdpn apegtdgevp kkkkrgrrsk aeilaeqmrr dgapdlnats
     3421 ltssvahtva snisptvnla lnpavptimt pdaerpsegp ismmtgpdgq lfnpdgsplk
     3481 pkrrgrgkgk ktlaleaara aeaaakaave vgllsmgtds npelkpgeip nilptpgsst
     3541 sgsapstppa gvvttqgppa sqtpttqpvy pslppnqqss vitrmlqsqp vssspqsfsa
     3601 aaaamghkyf ggpntggqim ggprtgyemq prgripspyr qagqssipph faavrsgtpp
     3661 mrmrvpgpqi yhtphhpmdp spsgggpisi nsrdrssplg pgpamippaa gsplakggpt
     3721 ppppppyvrg applprftdn pmgprhqmpp ftnaspvnhn mqqpspppnr ppgnfspyhp
     3781 ppppnyhyga ypppppmsta ddaaayqgsp ypadhfsspa dnqppiqapp ppqppppppq
     3841 qqqahpndaa gvgnkqydee gsgefgglvs yfssqreddl ds
//
LOCUS       XP_034191260            3870 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609264 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191260
VERSION     XP_034191260.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335369.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191260.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3870
                     /product="uncharacterized protein LOC117609264 isoform X4"
                     /calculated_mol_wt=432494
     Region          <245..309
                     /region_name="DNA_pol_phi"
                     /note="DNA polymerase phi; pfam04931"
                     /db_xref="CDD:461488"
     Region          1293..>2192
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          2642..2687
                     /region_name="PHD_RSF1"
                     /note="PHD finger found in Remodeling and spacing factor 1
                     (Rsf-1); cd15543"
                     /db_xref="CDD:277018"
     Site            order(2642,2654..2658,2662,2682)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277018"
     Region          3517..3744
                     /region_name="MISS"
                     /note="MAPK-interacting and spindle-stabilising
                     protein-like; pfam15822"
                     /db_xref="CDD:318115"
     CDS             1..3870
                     /gene="LOC117609264"
                     /coded_by="XM_034335369.2:868..12480"
                     /db_xref="GeneID:117609264"
ORIGIN      
        1 masdneasca sdpnfavics flecfgkscg iiypdiarlq emlentqevp qelidlhikl
       61 lrktrktvsp ekweralvkf chtysnqdgw elerfgykka riavklrllk vlletqfdln
      121 qkfknevnkl aanelrvepl grdksglayw cqldeecnir vyredldeen welvakdrdg
      181 vvnlintlsn geigdipine dsnsleisek piidtgqitt spsledeslq engihaedlc
      241 dkqsnkhege dsedeqeqde fnegdniedd idgedeitne ddsskqntee ddsqetvqse
      301 ilesnlikaf plmtasskpl ngkinvlsen etvtsrkkki dtvpetagtv sdyksmdtck
      361 skehievnnv kvksgesvpl ksietsvits plklvnvset kqvdeekias gvtalnitkk
      421 lhnedssdqi lkpldklsgk sfsfssteih tahsklsvkp idqlaanlvr mqseklekpi
      481 gakslekiae nlarsgsivg nvingdddrl pqdfcarsqm ekanvharen rgmdlstspr
      541 gwestseqnk pmdfsgidls srkfnkpmdi lssgyrtqdf qhremdlstk klnkpevtsl
      601 pydaravmmr nhamvadlsk rqmsfpsydm ssvspyhnaa ripnkddhrl pnytilpdps
      661 klaalrmntt pvkrsleadd lqqdmlkrir advipiqrsi dkrpmigatw rdeigeavee
      721 pvmmiqgegs gsdcdavnpg lgeaveepii ffygegsgee cqtgnpgdst sadnkesnes
      781 kneadsatss lstnksfseg slinevsaes lvtnksaikl nrnypqsnin vksnfqivgs
      841 tqekpkfkpt lgiqiipknt nssvkrlsrw dvgkpeekpe cdssiisnee nisqrrnkie
      901 cedsssenqd vkidgnsisi edvtsqqsnv klenlqenig esssaieekq sstdqdsiqc
      961 cdssislcqa dtsetfksct ktksvtendk tniknvcdie stslhetsts ermdneckps
     1021 avspprfffg pncisytskt emfesqpdht vstsiietnk sdtlqyecvs ssrpaedtin
     1081 tyksddfdvt qtnnnslkvn lyphktanpv ceddnknkie kiegaanswe psasskysle
     1141 qpsklrktei slhdpesvve ieedstscti dtvqdsisst eaaskecikf ketemldtis
     1201 qphstndlin kkdvdmkmen avvipselel kqecnifeeq nkeesdsadd ivkslntsip
     1261 cseaepycks sefkenklea dsvssksscs ssdgiqnfqk nnftesedht setecfvyqe
     1321 tndlaekeld yisqktfdss vticeennen rddrkyenqn eeliefdnki ttdligsdae
     1381 avlvncnesn eeinrqnevl edsenegkkv fidpfiknfv pdvntkldsi dnstvtssef
     1441 iekcnkdtlv ptisssddsc ihqdtsfhes vklnsekssf kdecptydtl lpetvpdtni
     1501 snssrsnivd stkesnvvqa aiskgqssci dqdlnnlskd eegstklyeg eacsnenlss
     1561 nenayndslk lqspsletnd sgiisekdkt gnvnfveiqe kfdtviepnk tddrtqfcke
     1621 dvteyvqdsy qedqkqaivs niletnskta ekseieqeel khtkinpqel inseikqslv
     1681 anyessdfdd snddvfeafe tepiesksnf ssdleeivsd aeeneeknie kpskkneils
     1741 nevqgipvdk etesmidssg iekitvdkse ekekglneet dfnvvqhdln leialdekir
     1801 lvnseqnssn tvelaetets tisseinpik kecislelnk annliniike gkkessdevr
     1861 knlvdvvvpv ektdfkidat neietvelln nsnneiiped lrtelslkst eenlvtsldn
     1921 dvkfmkneev khvteeisii etcnkvrkss ehgdkenkfd inlqeeknfn keecnvsihs
     1981 vsnilplkfd niekseihkn nisvmsenfk pmdnlqyvsf mseksensdi idnhenrnks
     2041 lrvtnetide sisestvtsv kdsstsfstq qgmitsapii aindykkeas krfsdvsdli
     2101 qdvpplkskp pciepaneei lqdlsnskaq lnrnldnfke melavnetka rddmitvsdn
     2161 iavqstkdec hrtvanvlnn ilnqierkni etneekestd iqsveiktvs lisddslgad
     2221 nenlfgiase emdplactdd dddddddddd ddndddddgt vkklddtpki alrvkpvsel
     2281 vydgwkldst eppklsrkrr nsshesnsed ggmkedevim mggkrmklrg krmadkqlrk
     2341 sveesrvvti ssedeavkcd dsqelkeqes kdasddqsga hlsaidrksr grprgrrrrg
     2401 ykgsgrsnrs khsefqssqv setspevife gtppsnndsl nmtpvsqkkr kkrkmvlgle
     2461 vgrdivpepe agltqdetpv rqsrriaqlk ikeaqlkiee eslsekkerk dkkekkdste
     2521 kkkrkkqkpe seeeiiikdi ekekkskkkr rkrkkkkmls kfneanpwqs ssgsssdeen
     2581 endeeeeeee diesegsllf ksdhefspes dlekdqesep lrrartaqka qsdveeadde
     2641 yacqkcgkad hpewillcds cdkgwhcscl rpalmlipeg dwfcppcqhn llvvklrenl
     2701 ktydqltkrh enevlrkkrl afvgisldnv lhknetqrhq kesnassqes dnesttttst
     2761 sstetsssee sepvyqlrer rcanrykfne ydaiinaaiq deveavqgag nqgrgkdiat
     2821 ivnakkeeae lefkneilnm deipankddi erksekdsde eykidaedgv deeeeeqhkg
     2881 tvkkflsrkk hrklnsldis seddpesded fkgtsseeee dfddhitssd dssfadmkrr
     2941 grkdsrsvrr strvrtmrye edfinddsee sdrpkrkksr stwdsdneds dnswrqrkkk
     3001 sktiptskyk tlpktksrkk kkrkriiesd nqsdnddste eqkietscvi qaqlennesv
     3061 tldalnmkve nvnennenkn vnemndikle emktevadis vtlplqdall qkkkkenatk
     3121 pkktptirrk iiygglpden kpeeeetlgr rtrgrkmnyq eeiasdseee lkkalrrtge
     3181 sedefvvneg edliedaekd sdsgdiynpk kdghkvknks pksrkarksk spggkrkmln
     3241 dgtpkprkkp gpkpgsknks rkqnflmgsm iqrrddqdee kdimgsftgd vpegsltglg
     3301 pgeladldde qleqmmmede eygrrqlela aieiakkkkk eereakklek arlkaleila
     3361 aerqrdpnap egtdgevpkk kkrgrrskae ilaeqmrrdg apdlnatslt ssvahtvasn
     3421 isptvnlaln pavptimtpd aerpsegpis mmtgpdgqlf npdgsplkpk rrgrgkgkkt
     3481 laleaaraae aaakaavevg llsmgtdsnp elkpgeipni lptpgsstsg sapstppagv
     3541 vttqgppasq tpttqpvyps lppnqqssvi trmlqsqpvs sspqsfsaaa aamghkyfgg
     3601 pntggqimgg prtgyemqpr gripspyrqa gqssipphfa avrsgtppmr mrvpgpqiyh
     3661 tphhpmdpsp sgggpisins rdrssplgpg pamippaags plakggptpp ppppyvrgap
     3721 plprftdnpm gprhqmppft naspvnhnmq qpspppnrpp gnfspyhppp ppnyhygayp
     3781 ppppmstadd aaayqgspyp adhfsspadn qppiqapppp qppppppqqq qahpndaagv
     3841 gnkqydeegs gefgglvsyf ssqreddlds
//
LOCUS       XP_034191262            3864 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609264 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191262
VERSION     XP_034191262.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335371.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191262.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3864
                     /product="uncharacterized protein LOC117609264 isoform X5"
                     /calculated_mol_wt=431694
     Region          <245..309
                     /region_name="DNA_pol_phi"
                     /note="DNA polymerase phi; pfam04931"
                     /db_xref="CDD:461488"
     Region          1293..>2192
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          2642..2687
                     /region_name="PHD_RSF1"
                     /note="PHD finger found in Remodeling and spacing factor 1
                     (Rsf-1); cd15543"
                     /db_xref="CDD:277018"
     Site            order(2642,2654..2658,2662,2682)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277018"
     Region          3530..3757
                     /region_name="MISS"
                     /note="MAPK-interacting and spindle-stabilising
                     protein-like; pfam15822"
                     /db_xref="CDD:318115"
     CDS             1..3864
                     /gene="LOC117609264"
                     /coded_by="XM_034335371.2:868..12462"
                     /db_xref="GeneID:117609264"
ORIGIN      
        1 masdneasca sdpnfavics flecfgkscg iiypdiarlq emlentqevp qelidlhikl
       61 lrktrktvsp ekweralvkf chtysnqdgw elerfgykka riavklrllk vlletqfdln
      121 qkfknevnkl aanelrvepl grdksglayw cqldeecnir vyredldeen welvakdrdg
      181 vvnlintlsn geigdipine dsnsleisek piidtgqitt spsledeslq engihaedlc
      241 dkqsnkhege dsedeqeqde fnegdniedd idgedeitne ddsskqntee ddsqetvqse
      301 ilesnlikaf plmtasskpl ngkinvlsen etvtsrkkki dtvpetagtv sdyksmdtck
      361 skehievnnv kvksgesvpl ksietsvits plklvnvset kqvdeekias gvtalnitkk
      421 lhnedssdqi lkpldklsgk sfsfssteih tahsklsvkp idqlaanlvr mqseklekpi
      481 gakslekiae nlarsgsivg nvingdddrl pqdfcarsqm ekanvharen rgmdlstspr
      541 gwestseqnk pmdfsgidls srkfnkpmdi lssgyrtqdf qhremdlstk klnkpevtsl
      601 pydaravmmr nhamvadlsk rqmsfpsydm ssvspyhnaa ripnkddhrl pnytilpdps
      661 klaalrmntt pvkrsleadd lqqdmlkrir advipiqrsi dkrpmigatw rdeigeavee
      721 pvmmiqgegs gsdcdavnpg lgeaveepii ffygegsgee cqtgnpgdst sadnkesnes
      781 kneadsatss lstnksfseg slinevsaes lvtnksaikl nrnypqsnin vksnfqivgs
      841 tqekpkfkpt lgiqiipknt nssvkrlsrw dvgkpeekpe cdssiisnee nisqrrnkie
      901 cedsssenqd vkidgnsisi edvtsqqsnv klenlqenig esssaieekq sstdqdsiqc
      961 cdssislcqa dtsetfksct ktksvtendk tniknvcdie stslhetsts ermdneckps
     1021 avspprfffg pncisytskt emfesqpdht vstsiietnk sdtlqyecvs ssrpaedtin
     1081 tyksddfdvt qtnnnslkvn lyphktanpv ceddnknkie kiegaanswe psasskysle
     1141 qpsklrktei slhdpesvve ieedstscti dtvqdsisst eaaskecikf ketemldtis
     1201 qphstndlin kkdvdmkmen avvipselel kqecnifeeq nkeesdsadd ivkslntsip
     1261 cseaepycks sefkenklea dsvssksscs ssdgiqnfqk nnftesedht setecfvyqe
     1321 tndlaekeld yisqktfdss vticeennen rddrkyenqn eeliefdnki ttdligsdae
     1381 avlvncnesn eeinrqnevl edsenegkkv fidpfiknfv pdvntkldsi dnstvtssef
     1441 iekcnkdtlv ptisssddsc ihqdtsfhes vklnsekssf kdecptydtl lpetvpdtni
     1501 snssrsnivd stkesnvvqa aiskgqssci dqdlnnlskd eegstklyeg eacsnenlss
     1561 nenayndslk lqspsletnd sgiisekdkt gnvnfveiqe kfdtviepnk tddrtqfcke
     1621 dvteyvqdsy qedqkqaivs niletnskta ekseieqeel khtkinpqel inseikqslv
     1681 anyessdfdd snddvfeafe tepiesksnf ssdleeivsd aeeneeknie kpskkneils
     1741 nevqgipvdk etesmidssg iekitvdkse ekekglneet dfnvvqhdln leialdekir
     1801 lvnseqnssn tvelaetets tisseinpik kecislelnk annliniike gkkessdevr
     1861 knlvdvvvpv ektdfkidat neietvelln nsnneiiped lrtelslkst eenlvtsldn
     1921 dvkfmkneev khvteeisii etcnkvrkss ehgdkenkfd inlqeeknfn keecnvsihs
     1981 vsnilplkfd niekseihkn nisvmsenfk pmdnlqyvsf mseksensdi idnhenrnks
     2041 lrvtnetide sisestvtsv kdsstsfstq qgmitsapii aindykkeas krfsdvsdli
     2101 qdvpplkskp pciepaneei lqdlsnskaq lnrnldnfke melavnetka rddmitvsdn
     2161 iavqstkdec hrtvanvlnn ilnqierkni etneekestd iqsveiktvs lisddslgad
     2221 nenlfgiase emdplactdd dddddddddd ddndddddgt vkklddtpki alrvkpvsel
     2281 vydgwkldst eppklsrkrr nsshesnsed ggmkedevim mggkrmklrg krmadkqlrk
     2341 sveesrvvti ssedeavkcd dsqelkeqes kdasddqsga hlsaidrksr grprgrrrrg
     2401 ykgsgrsnrs khsefqssqv setspevife gtppsnndsl nmtpvsqkkr kkrkmvlgle
     2461 vgrdivpepe agltqdetpv rqsrriaqlk ikeaqlkiee eslsekkerk dkkekkdste
     2521 kkkrkkqkpe seeeiiikdi ekekkskkkr rkrkkkkmls kfneanpwqs ssgsssdeen
     2581 endeeeeeee diesegsllf ksdhefspes dlekdqesep lrrartaqka qsdveeadde
     2641 yacqkcgkad hpewillcds cdkgwhcscl rpalmlipeg dwfcppcqhn llvvklrenl
     2701 ktydqltkrh enevlrkkrl afvgisldnv lhknetqrhq kesnassqes dnesttttst
     2761 sstetsssee sepvyqlrer rcanrykfne ydaiinaaiq deveavqgag nqgrgkdiat
     2821 ivnakkeeae lefkneilnm deipankddi erksekdsde eykidaedgv deeeeeqhkg
     2881 tvkkflsrkk hrklnsldis seddpesded fkgtsseeee dfddhitssd dssfadmkrr
     2941 grkdsrsvrr strvrtmrye edfinddsee sdrpkrkksr stwdsdneds dnswrqrkkk
     3001 sktiptskyk tlpktksrkk kkrkriiesd nqsdnddste eqkietscvi qaqlennesv
     3061 tldalnmkve nvnennenkn vnemndikle emktevadis vtlplqdall qkkkkenatk
     3121 pkktptirrk iiygglpden kpeeeetlgr rtrgrkmnyq eeiasdseee lkkalrrtge
     3181 sedefvvneg edliedaekd sdsgdiynpk kdghkvknks pksrkarksk spggkrkmln
     3241 dgtpkprkkp gpkpgsknks rkqnflmgsm iqrrddqdee kdimenvglt stamsvagsf
     3301 tgdvpegslt glgpgeladl ddeqleqmmm edeeygrrql elaaieiakk kkkeereakk
     3361 lekarlkale ilaaerqrdp napegtdgev pkkkkrgrrs kaeilaeqmr rdgapdlnat
     3421 sltssvahtv asnisptvnl alnpavptim tpdaerpseg pismmtgpdg qlfnpdgspl
     3481 kpkrrgrgkg kktlaleaar aaeaaakaav evgllsmgtd snpelkpgei pnilptpgss
     3541 tsgsapstpp agvvttqgpp asqtpttqpv ypslppnqqs svitrmlqsq pvssspqsfs
     3601 aaaaamghky fggpntggqi mggprtgyem qprgripspy rqagqssipp hfaavrsgtp
     3661 pmrmrvpgpq iyhtphhpmd pspsgggpis insrdrsspl gpgpamippa agsplakggp
     3721 tppppppyvr gapplprftd npmgprhqmp pftnaspvnh nmqqpspppn rppgnfspyh
     3781 pppppnyhyg aypppppmst addaaayqgs pypadhfssp adnqppiqap pppqpppppp
     3841 qqqqahpnda agvgnkqyde egse
//
LOCUS       XP_034191263            3835 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609264 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191263
VERSION     XP_034191263.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335372.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191263.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3835
                     /product="uncharacterized protein LOC117609264 isoform X6"
                     /calculated_mol_wt=428961
     Region          <245..309
                     /region_name="DNA_pol_phi"
                     /note="DNA polymerase phi; pfam04931"
                     /db_xref="CDD:461488"
     Region          1293..>2192
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          2642..2687
                     /region_name="PHD_RSF1"
                     /note="PHD finger found in Remodeling and spacing factor 1
                     (Rsf-1); cd15543"
                     /db_xref="CDD:277018"
     Site            order(2642,2654..2658,2662,2682)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277018"
     Region          <3366..3773
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..3835
                     /gene="LOC117609264"
                     /coded_by="XM_034335372.2:868..12375"
                     /db_xref="GeneID:117609264"
ORIGIN      
        1 masdneasca sdpnfavics flecfgkscg iiypdiarlq emlentqevp qelidlhikl
       61 lrktrktvsp ekweralvkf chtysnqdgw elerfgykka riavklrllk vlletqfdln
      121 qkfknevnkl aanelrvepl grdksglayw cqldeecnir vyredldeen welvakdrdg
      181 vvnlintlsn geigdipine dsnsleisek piidtgqitt spsledeslq engihaedlc
      241 dkqsnkhege dsedeqeqde fnegdniedd idgedeitne ddsskqntee ddsqetvqse
      301 ilesnlikaf plmtasskpl ngkinvlsen etvtsrkkki dtvpetagtv sdyksmdtck
      361 skehievnnv kvksgesvpl ksietsvits plklvnvset kqvdeekias gvtalnitkk
      421 lhnedssdqi lkpldklsgk sfsfssteih tahsklsvkp idqlaanlvr mqseklekpi
      481 gakslekiae nlarsgsivg nvingdddrl pqdfcarsqm ekanvharen rgmdlstspr
      541 gwestseqnk pmdfsgidls srkfnkpmdi lssgyrtqdf qhremdlstk klnkpevtsl
      601 pydaravmmr nhamvadlsk rqmsfpsydm ssvspyhnaa ripnkddhrl pnytilpdps
      661 klaalrmntt pvkrsleadd lqqdmlkrir advipiqrsi dkrpmigatw rdeigeavee
      721 pvmmiqgegs gsdcdavnpg lgeaveepii ffygegsgee cqtgnpgdst sadnkesnes
      781 kneadsatss lstnksfseg slinevsaes lvtnksaikl nrnypqsnin vksnfqivgs
      841 tqekpkfkpt lgiqiipknt nssvkrlsrw dvgkpeekpe cdssiisnee nisqrrnkie
      901 cedsssenqd vkidgnsisi edvtsqqsnv klenlqenig esssaieekq sstdqdsiqc
      961 cdssislcqa dtsetfksct ktksvtendk tniknvcdie stslhetsts ermdneckps
     1021 avspprfffg pncisytskt emfesqpdht vstsiietnk sdtlqyecvs ssrpaedtin
     1081 tyksddfdvt qtnnnslkvn lyphktanpv ceddnknkie kiegaanswe psasskysle
     1141 qpsklrktei slhdpesvve ieedstscti dtvqdsisst eaaskecikf ketemldtis
     1201 qphstndlin kkdvdmkmen avvipselel kqecnifeeq nkeesdsadd ivkslntsip
     1261 cseaepycks sefkenklea dsvssksscs ssdgiqnfqk nnftesedht setecfvyqe
     1321 tndlaekeld yisqktfdss vticeennen rddrkyenqn eeliefdnki ttdligsdae
     1381 avlvncnesn eeinrqnevl edsenegkkv fidpfiknfv pdvntkldsi dnstvtssef
     1441 iekcnkdtlv ptisssddsc ihqdtsfhes vklnsekssf kdecptydtl lpetvpdtni
     1501 snssrsnivd stkesnvvqa aiskgqssci dqdlnnlskd eegstklyeg eacsnenlss
     1561 nenayndslk lqspsletnd sgiisekdkt gnvnfveiqe kfdtviepnk tddrtqfcke
     1621 dvteyvqdsy qedqkqaivs niletnskta ekseieqeel khtkinpqel inseikqslv
     1681 anyessdfdd snddvfeafe tepiesksnf ssdleeivsd aeeneeknie kpskkneils
     1741 nevqgipvdk etesmidssg iekitvdkse ekekglneet dfnvvqhdln leialdekir
     1801 lvnseqnssn tvelaetets tisseinpik kecislelnk annliniike gkkessdevr
     1861 knlvdvvvpv ektdfkidat neietvelln nsnneiiped lrtelslkst eenlvtsldn
     1921 dvkfmkneev khvteeisii etcnkvrkss ehgdkenkfd inlqeeknfn keecnvsihs
     1981 vsnilplkfd niekseihkn nisvmsenfk pmdnlqyvsf mseksensdi idnhenrnks
     2041 lrvtnetide sisestvtsv kdsstsfstq qgmitsapii aindykkeas krfsdvsdli
     2101 qdvpplkskp pciepaneei lqdlsnskaq lnrnldnfke melavnetka rddmitvsdn
     2161 iavqstkdec hrtvanvlnn ilnqierkni etneekestd iqsveiktvs lisddslgad
     2221 nenlfgiase emdplactdd dddddddddd ddndddddgt vkklddtpki alrvkpvsel
     2281 vydgwkldst eppklsrkrr nsshesnsed ggmkedevim mggkrmklrg krmadkqlrk
     2341 sveesrvvti ssedeavkcd dsqelkeqes kdasddqsga hlsaidrksr grprgrrrrg
     2401 ykgsgrsnrs khsefqssqv setspevife gtppsnndsl nmtpvsqkkr kkrkmvlgle
     2461 vgrdivpepe agltqdetpv rqsrriaqlk ikeaqlkiee eslsekkerk dkkekkdste
     2521 kkkrkkqkpe seeeiiikdi ekekkskkkr rkrkkkkmls kfneanpwqs ssgsssdeen
     2581 endeeeeeee diesegsllf ksdhefspes dlekdqesep lrrartaqka qsdveeadde
     2641 yacqkcgkad hpewillcds cdkgwhcscl rpalmlipeg dwfcppcqhn llvvklrenl
     2701 ktydqltkrh enevlrkkrl afvgisldnv lhknetqrhq kesnassqes dnesttttst
     2761 sstetsssee sepvyqlrer rcanrykfne ydaiinaaiq deveavqgag nqgrgkdiat
     2821 ivnakkeeae lefkneilnm deipankddi erksekdsde eykidaedgv deeeeeqhkg
     2881 tvkkflsrkk hrklnsldis seddpesded fkgtsseeee dfddhitssd dssfadmkrr
     2941 grkdsrsvrr strvrtmrye edfinddsee sdrpkrkksr stwdsdneds dnswrqrkkk
     3001 sktiptskyk tlpktksrkk kkrkriiesd nqsdnddste eqkietscvi qaqlennesv
     3061 tldalnmkve nvnennenkn vnemndikle emktevadis vtlplqdall qkkkkenatk
     3121 pkktptirrk iiygglpden kpeeeetlgr rtrgrkmnyq eeiasdseee lkkalrrtge
     3181 sedefvvneg edliedaekd sdsgdiynpk kdghkvknks pksrkarksk spggkrkmln
     3241 dgtpkprkkp gpkpgsknks rkqnflmgsm iqrrddqdee kdimenvglt stamsvagsf
     3301 tgdvpegslt glgpgeladl ddeqleqmmm edeeygrrql elaaieiakk kkkeereakk
     3361 lekarlkale ilaaerqrdp napegtdgev pkkkkrgrrs kaeilaeqmr rdgapdlnat
     3421 sltssvahtv asnisptvnl alnpavptim tpdaerpseg pismmtgpdg qlfnpdgspl
     3481 kpkrrgrgkg sstsgsapst ppagvvttqg ppasqtpttq pvypslppnq qssvitrmlq
     3541 sqpvssspqs fsaaaaamgh kyfggpntgg qimggprtgy emqprgrips pyrqagqssi
     3601 pphfaavrsg tppmrmrvpg pqiyhtphhp mdpspsgggp isinsrdrss plgpgpamip
     3661 paagsplakg gptppppppy vrgapplprf tdnpmgprhq mppftnaspv nhnmqqpspp
     3721 pnrppgnfsp yhpppppnyh ygaypppppm staddaaayq gspypadhfs spadnqppiq
     3781 appppqpppp ppqqqqahpn daagvgnkqy deegsgefgg lvsyfssqre ddlds
//
LOCUS       XP_034191264            3770 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609264 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191264
VERSION     XP_034191264.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335373.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191264.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..3770
                     /product="uncharacterized protein LOC117609264 isoform X7"
                     /calculated_mol_wt=422525
     Region          <245..309
                     /region_name="DNA_pol_phi"
                     /note="DNA polymerase phi; pfam04931"
                     /db_xref="CDD:461488"
     Region          1293..>2192
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          2642..2687
                     /region_name="PHD_RSF1"
                     /note="PHD finger found in Remodeling and spacing factor 1
                     (Rsf-1); cd15543"
                     /db_xref="CDD:277018"
     Site            order(2642,2654..2658,2662,2682)
                     /site_type="other"
                     /note="putative histone H3 binding site [polypeptide
                     binding]"
                     /db_xref="CDD:277018"
     Region          3474..3644
                     /region_name="MISS"
                     /note="MAPK-interacting and spindle-stabilising
                     protein-like; pfam15822"
                     /db_xref="CDD:318115"
     CDS             1..3770
                     /gene="LOC117609264"
                     /coded_by="XM_034335373.2:868..12180"
                     /db_xref="GeneID:117609264"
ORIGIN      
        1 masdneasca sdpnfavics flecfgkscg iiypdiarlq emlentqevp qelidlhikl
       61 lrktrktvsp ekweralvkf chtysnqdgw elerfgykka riavklrllk vlletqfdln
      121 qkfknevnkl aanelrvepl grdksglayw cqldeecnir vyredldeen welvakdrdg
      181 vvnlintlsn geigdipine dsnsleisek piidtgqitt spsledeslq engihaedlc
      241 dkqsnkhege dsedeqeqde fnegdniedd idgedeitne ddsskqntee ddsqetvqse
      301 ilesnlikaf plmtasskpl ngkinvlsen etvtsrkkki dtvpetagtv sdyksmdtck
      361 skehievnnv kvksgesvpl ksietsvits plklvnvset kqvdeekias gvtalnitkk
      421 lhnedssdqi lkpldklsgk sfsfssteih tahsklsvkp idqlaanlvr mqseklekpi
      481 gakslekiae nlarsgsivg nvingdddrl pqdfcarsqm ekanvharen rgmdlstspr
      541 gwestseqnk pmdfsgidls srkfnkpmdi lssgyrtqdf qhremdlstk klnkpevtsl
      601 pydaravmmr nhamvadlsk rqmsfpsydm ssvspyhnaa ripnkddhrl pnytilpdps
      661 klaalrmntt pvkrsleadd lqqdmlkrir advipiqrsi dkrpmigatw rdeigeavee
      721 pvmmiqgegs gsdcdavnpg lgeaveepii ffygegsgee cqtgnpgdst sadnkesnes
      781 kneadsatss lstnksfseg slinevsaes lvtnksaikl nrnypqsnin vksnfqivgs
      841 tqekpkfkpt lgiqiipknt nssvkrlsrw dvgkpeekpe cdssiisnee nisqrrnkie
      901 cedsssenqd vkidgnsisi edvtsqqsnv klenlqenig esssaieekq sstdqdsiqc
      961 cdssislcqa dtsetfksct ktksvtendk tniknvcdie stslhetsts ermdneckps
     1021 avspprfffg pncisytskt emfesqpdht vstsiietnk sdtlqyecvs ssrpaedtin
     1081 tyksddfdvt qtnnnslkvn lyphktanpv ceddnknkie kiegaanswe psasskysle
     1141 qpsklrktei slhdpesvve ieedstscti dtvqdsisst eaaskecikf ketemldtis
     1201 qphstndlin kkdvdmkmen avvipselel kqecnifeeq nkeesdsadd ivkslntsip
     1261 cseaepycks sefkenklea dsvssksscs ssdgiqnfqk nnftesedht setecfvyqe
     1321 tndlaekeld yisqktfdss vticeennen rddrkyenqn eeliefdnki ttdligsdae
     1381 avlvncnesn eeinrqnevl edsenegkkv fidpfiknfv pdvntkldsi dnstvtssef
     1441 iekcnkdtlv ptisssddsc ihqdtsfhes vklnsekssf kdecptydtl lpetvpdtni
     1501 snssrsnivd stkesnvvqa aiskgqssci dqdlnnlskd eegstklyeg eacsnenlss
     1561 nenayndslk lqspsletnd sgiisekdkt gnvnfveiqe kfdtviepnk tddrtqfcke
     1621 dvteyvqdsy qedqkqaivs niletnskta ekseieqeel khtkinpqel inseikqslv
     1681 anyessdfdd snddvfeafe tepiesksnf ssdleeivsd aeeneeknie kpskkneils
     1741 nevqgipvdk etesmidssg iekitvdkse ekekglneet dfnvvqhdln leialdekir
     1801 lvnseqnssn tvelaetets tisseinpik kecislelnk annliniike gkkessdevr
     1861 knlvdvvvpv ektdfkidat neietvelln nsnneiiped lrtelslkst eenlvtsldn
     1921 dvkfmkneev khvteeisii etcnkvrkss ehgdkenkfd inlqeeknfn keecnvsihs
     1981 vsnilplkfd niekseihkn nisvmsenfk pmdnlqyvsf mseksensdi idnhenrnks
     2041 lrvtnetide sisestvtsv kdsstsfstq qgmitsapii aindykkeas krfsdvsdli
     2101 qdvpplkskp pciepaneei lqdlsnskaq lnrnldnfke melavnetka rddmitvsdn
     2161 iavqstkdec hrtvanvlnn ilnqierkni etneekestd iqsveiktvs lisddslgad
     2221 nenlfgiase emdplactdd dddddddddd ddndddddgt vkklddtpki alrvkpvsel
     2281 vydgwkldst eppklsrkrr nsshesnsed ggmkedevim mggkrmklrg krmadkqlrk
     2341 sveesrvvti ssedeavkcd dsqelkeqes kdasddqsga hlsaidrksr grprgrrrrg
     2401 ykgsgrsnrs khsefqssqv setspevife gtppsnndsl nmtpvsqkkr kkrkmvlgle
     2461 vgrdivpepe agltqdetpv rqsrriaqlk ikeaqlkiee eslsekkerk dkkekkdste
     2521 kkkrkkqkpe seeeiiikdi ekekkskkkr rkrkkkkmls kfneanpwqs ssgsssdeen
     2581 endeeeeeee diesegsllf ksdhefspes dlekdqesep lrrartaqka qsdveeadde
     2641 yacqkcgkad hpewillcds cdkgwhcscl rpalmlipeg dwfcppcqhn llvvklrenl
     2701 ktydqltkrh enevlrkkrl afvgisldnv lhknetqrhq kesnassqes dnesttttst
     2761 sstetsssee sepvyqlrer rcanrykfne ydaiinaaiq deveavqgag nqgrgkdiat
     2821 ivnakkeeae lefkneilnm deipankddi erksekdsde eykidaedgv deeeeeqhkg
     2881 tvkkflsrkk hrklnsldis seddpesded fkgtsseeee dfddhitssd dssfadmkrr
     2941 grkdsrsvrr strvrtmrye edfinddsee sdrpkrkksr stwdsdneds dnswrqrkkk
     3001 sktiptskyk tlpktksrkk kkrkriiesd nqsdnddste eqkietscvi qaqlennesv
     3061 tldalnmkve nvnennenkn vnemndikle emktevadis vtlplqdall qkkkkenatk
     3121 pkktptirrk iiygglpden kpeeeetlgr rtrgrkmnyq eeiasdseee lkkalrrtge
     3181 sedefvvneg edliedaekd sdsgdiynpk kdghkvknks pksrkarksk spggkrkmln
     3241 dgtpkprkkp gpkpgsknks rkqnflmgsm iqrrddqdee kdimenvglt stamsvagsf
     3301 tgdvpegslt glgpgeladl ddeqleqmmm edeeygrrql elaaieiakk kkkeereakk
     3361 lekarlkale ilaaerqrdp napegtdgev pkkkkrgrrs kaeilaeqmr rdgapdlnat
     3421 sltssvahtv asnisptvnl alnpavptim tpdaerpseg pismmtgpdg qlfnpdgspl
     3481 kpkrrgrgka aamghkyfgg pntggqimgg prtgyemqpr gripspyrqa gqssipphfa
     3541 avrsgtppmr mrvpgpqiyh tphhpmdpsp sgggpisins rdrssplgpg pamippaags
     3601 plakggptpp ppppyvrgap plprftdnpm gprhqmppft naspvnhnmq qpspppnrpp
     3661 gnfspyhppp ppnyhygayp ppppmstadd aaayqgspyp adhfsspadn qppiqapppp
     3721 qppppppqqq qahpndaagv gnkqydeegs gefgglvsyf ssqreddlds
//
LOCUS       XP_034191284            1993 aa            linear   INV 03-SEP-2025
DEFINITION  DNA polymerase zeta catalytic subunit [Osmia lignaria lignaria].
ACCESSION   XP_034191284
VERSION     XP_034191284.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335393.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191284.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1993
                     /product="DNA polymerase zeta catalytic subunit"
                     /calculated_mol_wt=228777
     Region          38..>184
                     /region_name="PTZ00166"
                     /note="DNA polymerase delta catalytic subunit;
                     Provisional"
                     /db_xref="CDD:240301"
     Region          <373..>885
                     /region_name="235kDa-fam"
                     /note="reticulocyte binding/rhoptry protein; TIGR01612"
                     /db_xref="CDD:130673"
     Region          1155..1382
                     /region_name="DNA_polB_zeta_exo"
                     /note="inactive DEDDy 3'-5' exonuclease domain of
                     eukaryotic DNA polymerase zeta, a family-B DNA polymerase;
                     cd05778"
                     /db_xref="CDD:99821"
     Region          1409..1858
                     /region_name="POLBc_zeta"
                     /note="DNA polymerase type-B zeta subfamily catalytic
                     domain. DNA polymerase (Pol) zeta is a member of the
                     eukaryotic B-family of DNA polymerases and distantly
                     related to DNA Pol delta. Pol zeta plays a major role in
                     translesion replication and the production...; cd05534"
                     /db_xref="CDD:99917"
     Site            order(1465,1468..1470,1548,1577,1581,1635)
                     /site_type="active"
                     /db_xref="CDD:99917"
     Site            order(1633,1635..1636)
                     /site_type="metal-binding"
                     /db_xref="CDD:99917"
     Region          1894..1960
                     /region_name="zf-C4pol"
                     /note="C4-type zinc-finger of DNA polymerase delta;
                     pfam14260"
                     /db_xref="CDD:464119"
     CDS             1..1993
                     /gene="DNApol-zeta"
                     /coded_by="XM_034335393.2:314..6295"
                     /db_xref="GeneID:117609269"
ORIGIN      
        1 msnmfsmsli tldsyqstpl selditfsef rgteikhvpv irifgstpsg iktclhihgv
       61 fpymyipcim knnvdsfmyk laaaidsain isfgftmsnt qhvykiqkvf giplygyhek
      121 ehlffkiyfy npamikraae llqngtilnq sfqpheahip filqfmidyn lygmslinlk
      181 yvkfrqsmyt efkenensls ladsqkylpi siirqstckl eadaqateil nrediqngld
      241 lnpgiaaiwn eekcrrdakg lqdagsqlly snadsriygl tendiyqeek lvqklqfvse
      301 indsvtpitp ktvsypleve nedsllstsd ilhhfkplis nkeskegtlf nhellqfeks
      361 tveetlpqdk nietstldse dmhlveilan laetneeeki vdddsmlasq fstftnqike
      421 dseddeienl nitsldldsl sswnsaakvs nqistskien kniddhtetd crieknvkrs
      481 lsinipqydg psdlflknks vskfsvldtv llkyfkiwqk rtrelkknrt ktmqtikqgl
      541 lsyknmsykh alhhqdldvy dideiehkls ltnyvnvqhk vsheintnqi nydkklhfmi
      601 nnnldklkip afdgnvdsss esdvdqdvti evqekkicty ksdtksrnen nvddslkkrk
      661 iefyisdvef spkkvrmlqn psslskkytp lnvkiispkr dkrvnernfi inepcssksv
      721 cntpkrkgte ekyptrntsg nanevllldd vsdstgtyke sidletehyn daskikslih
      781 ttfksnrevr kklnfmnekv adrinddiee yhfenlplte avinkvhkns dspniseiyn
      841 enlikilykt qdgkvhheyd ipntskqnin tyvddlknet lqinendede edicnmtytq
      901 ylnrkleies ntptplsldv tkrrlitiit kfnppskeri insmkiygcc rsksvapffs
      961 nkvdlikqke nsnkindvvp fkccldrvtg ikiwrrvkin efypsgssik scnikrvlag
     1021 ynpliihsli hppspknvkt wlqakkyllk ieskhkvikd rriditgnlv netciddahe
     1081 kknspnshns qhsnrsndsv nsslrkmlen pllyknivtq qylgisygqi eytmkdhsgn
     1141 vadenlknar gltthqflti lsvevhvitq gkllpdpeyn tigaifyavy adipsspdve
     1201 qmehgailin sciknekvkn ihsastacpi syvsseedll nsliqlvryy dpdilvgwei
     1261 eflswgyifh rasrigfnnf mwqisrisnv tpisrgqipd kdnlsdtrip griildvwri
     1321 mrheiallny tfenvmynvm reriscpsfe tltnwwkhnt itvqwrvvth yvirvvgtlk
     1381 mllhldiigk tceharligi qfyeifsrgs qfrvesmmlr lakplryili spsiqqrakm
     1441 rapqalplim epqsvfytdp livldfqsly psiiiaynyc fstclgrieh iggnepfefg
     1501 aatlrvkrnt ikklqgkinf apcgvafvkp evrrgilprm lteilntrlm vkqsmklhgs
     1561 enqalqrmlh sqqlglklia nvtygytaan fsgrmpciei gdsvvskgre tleraikive
     1621 stskwgaqvv ygdtdslfil lpgkskkdaf ligaeiadtv taanpspvkl kfekvlqpsi
     1681 lqtkkrycgy myefpeqekp eylakgietv rrdgcpavak ilekslkilf dtkdislvkl
     1741 yvtrqfdkil rqkisiqdlt fakefrglsg ykasacvpal eltrrlmrkd praiprtger
     1801 vryvivagap nqplihcvrt pmevildegl npnfvyyitk viippinrcl nligidvntw
     1861 yremshrqtp dkavslfsdn qkltirqyfg nvmcaicgdq tqkdvctncv gkpnqtvtil
     1921 heklrwlert yheltticqs ctnylgepec esldcpvlyr laqaqrnlvq vpylnqiicn
     1981 idnlyteern mkq
//
LOCUS       XP_034191373             616 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 11 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191373
VERSION     XP_034191373.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335482.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191373.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..616
                     /product="tyrosine-protein phosphatase non-receptor type
                     11 isoform X1"
                     /calculated_mol_wt=69597
     Region          5..103
                     /region_name="SH2_N-SH2_SHP_like"
                     /note="N-terminal Src homology 2 (N-SH2) domain found in
                     SH2 domain Phosphatases (SHP) proteins; cd10340"
                     /db_xref="CDD:198203"
     Site            order(13,32,53,55)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198203"
     Site            order(52..54,68,88..91,93)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198203"
     Region          111..208
                     /region_name="SH2_C-SH2_SHP_like"
                     /note="C-terminal Src homology 2 (C-SH2) domain found in
                     SH2 domain Phosphatases (SHP) proteins; cd09931"
                     /db_xref="CDD:198185"
     Site            order(139,141,146..147)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198185"
     Site            order(160,187)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198185"
     Region          281..553
                     /region_name="PTPc-N11_6"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 11 and type 6; cd14544"
                     /db_xref="CDD:350392"
     CDS             1..616
                     /gene="LOC117609314"
                     /coded_by="XM_034335482.2:259..2109"
                     /db_xref="GeneID:117609314"
ORIGIN      
        1 masrrwfhpn isgleaerll mergydssfl arpsssnpgd ftlsvrrnge vthikiqntg
       61 dfydlyggek fatlselvqf ymenggqlre kngevielky plncadptte rwfhghlsak
      121 eaerlmlerg kngsflvres qskpgdfvls vrtddrvthv iirsqdnkyd vgggdkfdsl
      181 sdliehykrn pmvetsgsvv hlrqpfnatr inasgiesrv rqlhkengcs ngwlwngatg
      241 sneggtgrgk gkagfweefe slqqlecrhl fsrkeglrpe nraknrykni lpfdhtrvrl
      301 kdvdpsipga dyinanyirn eegdsqtsgi naggdsgsfn kcyiatqgcl pntihdfwhm
      361 vyqentrviv mttkeiergk nkcarywpee geiteyggew kvralsrtst adytlrefll
      421 hgskpcftes rriyhyhfqa wpdhgvpsdp gcvlnflhdv narqesiats ltsngqsvas
      481 igpilvhcsa gigrtgtfiv idmildqikr hgldceidiq rtiqrvrsqr sgmvqteaqy
      541 kfvylavlhy ietvsqrmqa eqkslhlgre ytnirykset nvttmgdipv pacttttisa
      601 sthftlptsi snlrpk
//
LOCUS       XP_034191374             596 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191374
VERSION     XP_034191374.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335483.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191374.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="tyrosine-protein phosphatase non-receptor type
                     11 isoform X2"
                     /calculated_mol_wt=67232
     Region          2..83
                     /region_name="SH2_N-SH2_SHP_like"
                     /note="N-terminal Src homology 2 (N-SH2) domain found in
                     SH2 domain Phosphatases (SHP) proteins; cd10340"
                     /db_xref="CDD:198203"
     Site            order(32..34,48,68..71,73)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198203"
     Region          91..188
                     /region_name="SH2_C-SH2_SHP_like"
                     /note="C-terminal Src homology 2 (C-SH2) domain found in
                     SH2 domain Phosphatases (SHP) proteins; cd09931"
                     /db_xref="CDD:198185"
     Site            order(119,121,126..127)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198185"
     Site            order(140,167)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198185"
     Region          261..533
                     /region_name="PTPc-N11_6"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 11 and type 6; cd14544"
                     /db_xref="CDD:350392"
     CDS             1..596
                     /gene="LOC117609314"
                     /coded_by="XM_034335483.2:309..2099"
                     /db_xref="GeneID:117609314"
ORIGIN      
        1 mergydssfl arpsssnpgd ftlsvrrnge vthikiqntg dfydlyggek fatlselvqf
       61 ymenggqlre kngevielky plncadptte rwfhghlsak eaerlmlerg kngsflvres
      121 qskpgdfvls vrtddrvthv iirsqdnkyd vgggdkfdsl sdliehykrn pmvetsgsvv
      181 hlrqpfnatr inasgiesrv rqlhkengcs ngwlwngatg sneggtgrgk gkagfweefe
      241 slqqlecrhl fsrkeglrpe nraknrykni lpfdhtrvrl kdvdpsipga dyinanyirn
      301 eegdsqtsgi naggdsgsfn kcyiatqgcl pntihdfwhm vyqentrviv mttkeiergk
      361 nkcarywpee geiteyggew kvralsrtst adytlrefll hgskpcftes rriyhyhfqa
      421 wpdhgvpsdp gcvlnflhdv narqesiats ltsngqsvas igpilvhcsa gigrtgtfiv
      481 idmildqikr hgldceidiq rtiqrvrsqr sgmvqteaqy kfvylavlhy ietvsqrmqa
      541 eqkslhlgre ytnirykset nvttmgdipv pacttttisa sthftlptsi snlrpk
//
LOCUS       XP_034191375             596 aa            linear   INV 03-SEP-2025
DEFINITION  tyrosine-protein phosphatase non-receptor type 11 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191375
VERSION     XP_034191375.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335484.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191375.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..596
                     /product="tyrosine-protein phosphatase non-receptor type
                     11 isoform X2"
                     /calculated_mol_wt=67232
     Region          2..83
                     /region_name="SH2_N-SH2_SHP_like"
                     /note="N-terminal Src homology 2 (N-SH2) domain found in
                     SH2 domain Phosphatases (SHP) proteins; cd10340"
                     /db_xref="CDD:198203"
     Site            order(32..34,48,68..71,73)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198203"
     Region          91..188
                     /region_name="SH2_C-SH2_SHP_like"
                     /note="C-terminal Src homology 2 (C-SH2) domain found in
                     SH2 domain Phosphatases (SHP) proteins; cd09931"
                     /db_xref="CDD:198185"
     Site            order(119,121,126..127)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198185"
     Site            order(140,167)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198185"
     Region          261..533
                     /region_name="PTPc-N11_6"
                     /note="catalytic domain of tyrosine-protein phosphatase
                     non-receptor type 11 and type 6; cd14544"
                     /db_xref="CDD:350392"
     CDS             1..596
                     /gene="LOC117609314"
                     /coded_by="XM_034335484.2:177..1967"
                     /db_xref="GeneID:117609314"
ORIGIN      
        1 mergydssfl arpsssnpgd ftlsvrrnge vthikiqntg dfydlyggek fatlselvqf
       61 ymenggqlre kngevielky plncadptte rwfhghlsak eaerlmlerg kngsflvres
      121 qskpgdfvls vrtddrvthv iirsqdnkyd vgggdkfdsl sdliehykrn pmvetsgsvv
      181 hlrqpfnatr inasgiesrv rqlhkengcs ngwlwngatg sneggtgrgk gkagfweefe
      241 slqqlecrhl fsrkeglrpe nraknrykni lpfdhtrvrl kdvdpsipga dyinanyirn
      301 eegdsqtsgi naggdsgsfn kcyiatqgcl pntihdfwhm vyqentrviv mttkeiergk
      361 nkcarywpee geiteyggew kvralsrtst adytlrefll hgskpcftes rriyhyhfqa
      421 wpdhgvpsdp gcvlnflhdv narqesiats ltsngqsvas igpilvhcsa gigrtgtfiv
      481 idmildqikr hgldceidiq rtiqrvrsqr sgmvqteaqy kfvylavlhy ietvsqrmqa
      541 eqkslhlgre ytnirykset nvttmgdipv pacttttisa sthftlptsi snlrpk
//
LOCUS       XP_034191376             545 aa            linear   INV 03-SEP-2025
DEFINITION  chaperonin containing TCP1 subunit 8 [Osmia lignaria lignaria].
ACCESSION   XP_034191376
VERSION     XP_034191376.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335485.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..545
                     /product="chaperonin containing TCP1 subunit 8"
                     /calculated_mol_wt=59101
     Region          10..530
                     /region_name="chaperonin_like"
                     /note="chaperonin_like superfamily. Chaperonins are
                     involved in productive folding of proteins. They share a
                     common general morphology, a double toroid of 2 stacked
                     rings, each composed of 7-9 subunits. There are 2 main
                     chaperonin groups. The symmetry of type I...; cl02777"
                     /db_xref="CDD:351886"
     Site            order(20,24,52..55,57,75,77,81,85,88,382,520..526)
                     /site_type="other"
                     /note="ring oligomerisation interface [polypeptide
                     binding]"
                     /db_xref="CDD:239457"
     Site            order(47..49,99,103,166,394,412,452,497,499)
                     /site_type="other"
                     /note="ATP/Mg binding site [chemical binding]"
                     /db_xref="CDD:239457"
     Site            order(432,450,459,461..462,465)
                     /site_type="active"
                     /note="stacking interactions [active]"
                     /db_xref="CDD:239457"
     CDS             1..545
                     /gene="CCT8"
                     /coded_by="XM_034335485.2:114..1751"
                     /db_xref="GeneID:117609315"
ORIGIN      
        1 malhvpkapg maqmlkegar yfsgleeavy rnitackqfa qtvrtaygpn gmnkmvinhi
       61 eklfvtndaa tiineleveh paaklivlas kmqeaevgdg tnfviifaga lleaaeellr
      121 lgittseiie gyeaslekal qilptlicyt vndykdekqi kagiktsims kqfgnedilt
      181 slvtracvsi lpekttfnvd nvrvckilgs gisssevvqg mvfkrhvegd vtrkdnakia
      241 vytcavdimq tetkgtvlik tadelmnfsr geesllesqi kaiadsgadv ivsggkfgdm
      301 alhymnkynl mavripskfd vrrlcktvga talprlipps keelgysdsv yidevgdtiv
      361 vkfalhgkds rvstiivrgs tenymddier aiddgvntfk gitkdgrfvp gagateiela
      421 tqlatyadal pgleqyavrr fatalevfpk tlaensgsha pellsklyaa hkednknygf
      481 viddkgaaln dtaeagiidl fltkqwslky avgvactvlk vdqiimakra ggpkvpgggv
      541 rddde
//
LOCUS       XP_034191265             522 aa            linear   INV 03-SEP-2025
DEFINITION  ada2a-containing complex component 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191265
VERSION     XP_034191265.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335374.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191265.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="ada2a-containing complex component 3 isoform X1"
                     /calculated_mol_wt=58178
     Region          <48..227
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          48..73
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..106
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(77,81..82,85..87,89..90,94,97,106,108,110,114..115,
                     118..120,122..123,127,130,139,141,143,147..148,151..153,
                     155..156,160,163)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          108..139
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          141..170
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..522
                     /gene="Atac3"
                     /coded_by="XM_034335374.2:184..1752"
                     /db_xref="GeneID:117609266"
ORIGIN      
        1 mqaetictnd iegldsdtli pveilscdvs hrarsqdals ivelgkqllf sakygdtdsv
       61 rdlmcrgapf ttdwlgtsal hfaaqnnhte taevllragi srdartkvdr tplhmaayeg
      121 hhqmaqllln ygadvdsrdm lkmtplhwav erehvevmhv llehgadana tskfdktpis
      181 lalehdrldl vdilqqerev vgtrihqqsq ansaelevat hnliqleser deekqkfelp
      241 qqesqqrrki tqvqvgkkpr mifqqihvgs nadvtqekei envdeitnan nvnnnkkrkd
      301 vttvggvnkq frlleahgit mipvdndasi venamesgrt vvlteagkla lnltrntsig
      361 mkrlhvsgkk gaprkviair adqilnhntp tipsrgpnil krssmdnkag klyissisna
      421 ssvsthtqsk niisasenki insptkmaes iilqlddgie eiteddttdn nepvmdiavl
      481 nrqlaearrq aaeyrkrlqk keeeaeiykq qlknitaqra sk
//
LOCUS       XP_076548207             522 aa            linear   INV 03-SEP-2025
DEFINITION  ada2a-containing complex component 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548207
VERSION     XP_076548207.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692092.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..522
                     /product="ada2a-containing complex component 3 isoform X1"
                     /calculated_mol_wt=58178
     Region          <48..227
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          48..73
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..106
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(77,81..82,85..87,89..90,94,97,106,108,110,114..115,
                     118..120,122..123,127,130,139,141,143,147..148,151..153,
                     155..156,160,163)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          108..139
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          141..170
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..522
                     /gene="Atac3"
                     /coded_by="XM_076692092.1:76..1644"
                     /db_xref="GeneID:117609266"
ORIGIN      
        1 mqaetictnd iegldsdtli pveilscdvs hrarsqdals ivelgkqllf sakygdtdsv
       61 rdlmcrgapf ttdwlgtsal hfaaqnnhte taevllragi srdartkvdr tplhmaayeg
      121 hhqmaqllln ygadvdsrdm lkmtplhwav erehvevmhv llehgadana tskfdktpis
      181 lalehdrldl vdilqqerev vgtrihqqsq ansaelevat hnliqleser deekqkfelp
      241 qqesqqrrki tqvqvgkkpr mifqqihvgs nadvtqekei envdeitnan nvnnnkkrkd
      301 vttvggvnkq frlleahgit mipvdndasi venamesgrt vvlteagkla lnltrntsig
      361 mkrlhvsgkk gaprkviair adqilnhntp tipsrgpnil krssmdnkag klyissisna
      421 ssvsthtqsk niisasenki insptkmaes iilqlddgie eiteddttdn nepvmdiavl
      481 nrqlaearrq aaeyrkrlqk keeeaeiykq qlknitaqra sk
//
LOCUS       XP_034191270             513 aa            linear   INV 03-SEP-2025
DEFINITION  ada2a-containing complex component 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191270
VERSION     XP_034191270.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335379.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191270.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..513
                     /product="ada2a-containing complex component 3 isoform X2"
                     /calculated_mol_wt=57182
     Region          <48..227
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          48..73
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..106
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(77,81..82,85..87,89..90,94,97,106,108,110,114..115,
                     118..120,122..123,127,130,139,141,143,147..148,151..153,
                     155..156,160,163)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          108..139
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          141..170
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..513
                     /gene="Atac3"
                     /coded_by="XM_034335379.2:184..1725"
                     /db_xref="GeneID:117609266"
ORIGIN      
        1 mqaetictnd iegldsdtli pveilscdvs hrarsqdals ivelgkqllf sakygdtdsv
       61 rdlmcrgapf ttdwlgtsal hfaaqnnhte taevllragi srdartkvdr tplhmaayeg
      121 hhqmaqllln ygadvdsrdm lkmtplhwav erehvevmhv llehgadana tskfdktpis
      181 lalehdrldl vdilqqerev vgtrihqqsq ansaelevat hnliqleser deekqkfelp
      241 qqesqqrrki tqvqvgkkpr mifqqihvgs nadvtqekei envdeitnan nvnnnkkrkd
      301 vttvggvnkq frlleahgit mipvdndasi venamesgrt vvlteagkla lnltrntsig
      361 mkrlhvsgkk gaprkviair adqilnhntp tipsrgpnil krssmdnkag klyissisna
      421 ssvsthtqsk niisasemae siilqlddgi eeiteddttd nnepvmdiav lnrqlaearr
      481 qaaeyrkrlq kkeeeaeiyk qqlknitaqr ask
//
LOCUS       XP_034191271             507 aa            linear   INV 03-SEP-2025
DEFINITION  ada2a-containing complex component 3 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191271
VERSION     XP_034191271.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335380.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191271.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="ada2a-containing complex component 3 isoform X3"
                     /calculated_mol_wt=56595
     Region          <48..227
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          48..73
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          76..106
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(77,81..82,85..87,89..90,94,97,106,108,110,114..115,
                     118..120,122..123,127,130,139,141,143,147..148,151..153,
                     155..156,160,163)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          108..139
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          141..170
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     CDS             1..507
                     /gene="Atac3"
                     /coded_by="XM_034335380.2:184..1707"
                     /db_xref="GeneID:117609266"
ORIGIN      
        1 mqaetictnd iegldsdtli pveilscdvs hrarsqdals ivelgkqllf sakygdtdsv
       61 rdlmcrgapf ttdwlgtsal hfaaqnnhte taevllragi srdartkvdr tplhmaayeg
      121 hhqmaqllln ygadvdsrdm lkmtplhwav erehvevmhv llehgadana tskfdktpis
      181 lalehdrldl vdilqqerev vgtrihqqsq ansaelevat hnliqleser deekqkfelp
      241 qqesqqrrki tqvqvgkkpr mifqqihvgs nadvtqekei envdeitnan nvnnnkkrkd
      301 vttvggvnkq frlleahgit mipvdndasi venamesgrt vvltgmkrlh vsgkkgaprk
      361 viairadqil nhntptipsr gpnilkrssm dnkagklyis sisnassvst htqskniisa
      421 senkiinspt kmaesiilql ddgieeited dttdnnepvm diavlnrqla earrqaaeyr
      481 krlqkkeeea eiykqqlkni taqrask
//
LOCUS       XP_034191377             369 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC15B isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191377
VERSION     XP_034191377.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335486.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..369
                     /product="palmitoyltransferase ZDHHC15B isoform X1"
                     /calculated_mol_wt=43057
     Region          133..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..369
                     /gene="LOC117609316"
                     /coded_by="XM_034335486.2:490..1599"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 myicntasrm gkqngscwwf vkavkwipvi filtivlwsy yayvvqlcfy tadnyvqkvf
       61 ylffyhvlff lflwsywqtv ftdlieiptk fripdvemek fqqaeteevq rqilerfaqd
      121 lpitnrtikg virfcekcql ikpdrahhcs vcstcvlkmd hhcpwvnncv gfhnykffml
      181 flayallyci fitatslqyf icfwkgeldg mgrfhllflf fvalmfalsl nslffyhcyl
      241 vlhnrstlea ftppmfrtgk dkdgfslgky nnfqevfgdn pklwflpift slgngvtypv
      301 raqhqgtpnt ydsmgstrns fgdgvnfper lcdedthtll gpttqqwgde teldspppyg
      361 sqsgpplik
//
LOCUS       XP_034191378             366 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC15B isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191378
VERSION     XP_034191378.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335487.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..366
                     /product="palmitoyltransferase ZDHHC15B isoform X2"
                     /calculated_mol_wt=42749
     Region          133..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..366
                     /gene="LOC117609316"
                     /coded_by="XM_034335487.2:490..1590"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 myicntasrm gkqngscwwf vkavkwipvi filtivlwsy yayvvqlcfy tadnyvqkvf
       61 ylffyhvlff lflwsywqtv ftdlieiptk fripdvemek fqqaeteevq rqilerfaqd
      121 lpitnrtikg virfcekcql ikpdrahhcs vcstcvlkmd hhcpwvnncv gfhnykffml
      181 flayallyci fitatslqyf icfwkgeldg mgrfhllflf fvalmfalsl nslffyhcyl
      241 vlhnrstlea ftppmfrtgk dkdgfslgky nnfqevfgdn pklwflpift slgngvtypv
      301 raqhqgtpnt ydsmgstrns fgdgvnfper lcdedthtll gpttqqwgde teldspppyg
      361 sqsall
//
LOCUS       XP_034191379             361 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC15B isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034191379
VERSION     XP_034191379.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335488.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..361
                     /product="palmitoyltransferase ZDHHC15B isoform X3"
                     /calculated_mol_wt=41947
     Region          133..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..361
                     /gene="LOC117609316"
                     /coded_by="XM_034335488.2:490..1575"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 myicntasrm gkqngscwwf vkavkwipvi filtivlwsy yayvvqlcfy tadnyvqkvf
       61 ylffyhvlff lflwsywqtv ftdlieiptk fripdvemek fqqaeteevq rqilerfaqd
      121 lpitnrtikg virfcekcql ikpdrahhcs vcstcvlkmd hhcpwvnncv gfhnykffml
      181 flayallyci fitatslqyf icfwkgeldg mgrfhllflf fvalmfalsl nslffyhcyl
      241 vlhnrstlea ftppmfrtgk dkdgfslgky nnfqevfgdn pklwflpift slgngvtypv
      301 raqhqgtpnt ydsmgstrnr stfdqmtlve eatilalgtg tavigstada nqplvdatel
      361 v
//
LOCUS       XP_034191380             360 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC15B isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034191380
VERSION     XP_034191380.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335489.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="palmitoyltransferase ZDHHC15B isoform X4"
                     /calculated_mol_wt=42017
     Region          124..240
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..360
                     /gene="LOC117609316"
                     /coded_by="XM_034335489.2:157..1239"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 mgkqngscww fvkavkwipv ifiltivlws yyayvvqlcf ytadnyvqkv fylffyhvlf
       61 flflwsywqt vftdlieipt kfripdveme kfqqaeteev qrqilerfaq dlpitnrtik
      121 gvirfcekcq likpdrahhc svcstcvlkm dhhcpwvnnc vgfhnykffm lflayallyc
      181 ifitatslqy ficfwkgeld gmgrfhllfl ffvalmfals lnslffyhcy lvlhnrstle
      241 aftppmfrtg kdkdgfslgk ynnfqevfgd npklwflpif tslgngvtyp vraqhqgtpn
      301 tydsmgstrn sfgdgvnfpe rlcdedthtl lgpttqqwgd eteldspppy gsqsgpplik
//
LOCUS       XP_034191381             341 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC15B isoform X5 [Osmia lignaria lignaria].
ACCESSION   XP_034191381
VERSION     XP_034191381.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335490.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="palmitoyltransferase ZDHHC15B isoform X5"
                     /calculated_mol_wt=39799
     Region          133..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..341
                     /gene="LOC117609316"
                     /coded_by="XM_034335490.2:488..1513"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 myicntasrm gkqngscwwf vkavkwipvi filtivlwsy yayvvqlcfy tadnyvqkvf
       61 ylffyhvlff lflwsywqtv ftdlieiptk fripdvemek fqqaeteevq rqilerfaqd
      121 lpitnrtikg virfcekcql ikpdrahhcs vcstcvlkmd hhcpwvnncv gfhnykffml
      181 flayallyci fitatslqyf icfwkgeldg mgrfhllflf fvalmfalsl nslffyhcyl
      241 vlhnrstlea ftppmfrtgk dkdgfslgky nnfqevfgdn pklwflpift slgngvtypv
      301 raqhqgtpnt ydsmgstrns tavigstada nqplvdatel v
//
LOCUS       XP_034191382             340 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC15B isoform X6 [Osmia lignaria lignaria].
ACCESSION   XP_034191382
VERSION     XP_034191382.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335491.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..340
                     /product="palmitoyltransferase ZDHHC15B isoform X6"
                     /calculated_mol_wt=39966
     Region          133..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..340
                     /gene="LOC117609316"
                     /coded_by="XM_034335491.2:491..1513"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 myicntasrm gkqngscwwf vkavkwipvi filtivlwsy yayvvqlcfy tadnyvqkvf
       61 ylffyhvlff lflwsywqtv ftdlieiptk fripdvemek fqqaeteevq rqilerfaqd
      121 lpitnrtikg virfcekcql ikpdrahhcs vcstcvlkmd hhcpwvnncv gfhnykffml
      181 flayallyci fitatslqyf icfwkgeldg mgrfhllflf fvalmfalsl nslffyhcyl
      241 vlhnrstlea ftppmfrtgk dkdgfslgky nnfqevfgdn pklwflpift sfgdgvnfpe
      301 rlcdedthtl lgpttqqwgd eteldspppy gsqsgpplik
//
LOCUS       XP_076548208             337 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC15B isoform X7 [Osmia lignaria lignaria].
ACCESSION   XP_076548208
VERSION     XP_076548208.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692093.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..337
                     /product="palmitoyltransferase ZDHHC15B isoform X7"
                     /calculated_mol_wt=39629
     Region          133..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..337
                     /gene="LOC117609316"
                     /coded_by="XM_076692093.1:490..1503"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 myicntasrm gkqngscwwf vkavkwipvi filtivlwsy yayvvqlcfy tadnyvqkvf
       61 ylffyhvlff lflwsywqtv ftdlieiptk fripdvemek fqqaeteevq rqilerfaqd
      121 lpitnrtikg virfcekcql ikpdrahhcs vcstcvlkmd hhcpwvnncv gfhnykffml
      181 flayallyci fitatslqyf icfwkgeldg mgrfhllflf fvalmfalsl nslffyhcyl
      241 vlhnrstlea ftppmfrtgk dkdgfslgky nnfqevfgdn pklwflpift sfgdgvnfpe
      301 rlcdedthtl lgpttqqwgd eteldspppy gsqsgiv
//
LOCUS       XP_034191384             312 aa            linear   INV 03-SEP-2025
DEFINITION  palmitoyltransferase ZDHHC2 isoform X8 [Osmia lignaria lignaria].
ACCESSION   XP_034191384
VERSION     XP_034191384.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335493.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..312
                     /product="palmitoyltransferase ZDHHC2 isoform X8"
                     /calculated_mol_wt=36707
     Region          133..249
                     /region_name="DHHC"
                     /note="DHHC palmitoyltransferase; pfam01529"
                     /db_xref="CDD:396215"
     CDS             1..312
                     /gene="LOC117609316"
                     /coded_by="XM_034335493.2:490..1428"
                     /db_xref="GeneID:117609316"
ORIGIN      
        1 myicntasrm gkqngscwwf vkavkwipvi filtivlwsy yayvvqlcfy tadnyvqkvf
       61 ylffyhvlff lflwsywqtv ftdlieiptk fripdvemek fqqaeteevq rqilerfaqd
      121 lpitnrtikg virfcekcql ikpdrahhcs vcstcvlkmd hhcpwvnncv gfhnykffml
      181 flayallyci fitatslqyf icfwkgeldg mgrfhllflf fvalmfalsl nslffyhcyl
      241 vlhnrstlea ftppmfrtgk dkdgfslgky nnfqevfgdn pklwflpift stavigstad
      301 anqplvdate lv
//
LOCUS       XP_034191273             352 aa            linear   INV 03-SEP-2025
DEFINITION  dynein regulatory complex protein 9 [Osmia lignaria lignaria].
ACCESSION   XP_034191273
VERSION     XP_034191273.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335382.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191273.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="dynein regulatory complex protein 9"
                     /calculated_mol_wt=41676
     Region          <77..>312
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          305..344
                     /region_name="IQCG"
                     /note="IQ (isoleucine-glutamine) motif containing G
                     (IQCG); cd23766"
                     /db_xref="CDD:467744"
     CDS             1..352
                     /gene="LOC117609267"
                     /coded_by="XM_034335382.2:8..1066"
                     /db_xref="GeneID:117609267"
ORIGIN      
        1 maafaaneag ggssrfssse rvtilsmlee ytnalaiyhs tlkkptrqdn dilvncisfh
       61 skglltsvnl dktintehae ednvpeklyg esllyvrkvm enlkeeiren gtfemltnei
      121 eritchkkee eallqqqeel rktvaelqki iadekivnee kkkcilyels eeqrnikklk
      181 iiadaeeeyv marekakyeq nqlrcdteik klekmfndyr vqekneervh veltkfliqe
      241 talfeeqakq weeryvreke iyekeicqlr sdiesrqkel eqlkeeyhcn qgfidsclae
      301 kealkkqkvq eermrksavk iqawwrgimv rrklgpyrpk ekkrkrqika kk
//
LOCUS       XP_034191386             348 aa            linear   INV 03-SEP-2025
DEFINITION  arginine-hydroxylase NDUFAF5, mitochondrial isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191386
VERSION     XP_034191386.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335495.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191386.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..348
                     /product="arginine-hydroxylase NDUFAF5, mitochondrial
                     isoform X1"
                     /calculated_mol_wt=39419
     Region          59..308
                     /region_name="BioC"
                     /note="malonyl-acyl carrier protein O-methyltransferase
                     BioC; TIGR02072"
                     /db_xref="CDD:273953"
     CDS             1..348
                     /gene="LOC117609318"
                     /coded_by="XM_034335495.2:169..1215"
                     /db_xref="GeneID:117609318"
ORIGIN      
        1 mqaafkrsvr rfvfdiknar sinnsvinya lpadsvmyvf drntkllqre ratqapdvkv
       61 ydyikdevgs rladrifdik kkfkkaldlg cgrghvskhi laesveelil sdmsptilqq
      121 vkttegikvl rtvmdeenfe fepdsldliv sslslhwvnd lprcfknink slkndgvfma
      181 aifggetlye lrcslqlael erdggisahi spfaeirdig alltranftm ltidtdeivi
      241 gypsmfelmw dlkgmaenna arnrklrlnk dtilaaaaiy kelygklkdd gspyvpatfq
      301 viyllgwkpd plqpkplerg tgqvslkdly rldeiiketk kvktdedq
//
LOCUS       XP_034191387             344 aa            linear   INV 03-SEP-2025
DEFINITION  arginine-hydroxylase NDUFAF5, mitochondrial isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191387
VERSION     XP_034191387.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335496.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191387.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..344
                     /product="arginine-hydroxylase NDUFAF5, mitochondrial
                     isoform X2"
                     /calculated_mol_wt=38857
     Region          59..304
                     /region_name="BioC"
                     /note="malonyl-acyl carrier protein O-methyltransferase
                     BioC; TIGR02072"
                     /db_xref="CDD:273953"
     CDS             1..344
                     /gene="LOC117609318"
                     /coded_by="XM_034335496.2:110..1144"
                     /db_xref="GeneID:117609318"
ORIGIN      
        1 mqaafkrsvr rfvfdiknar sinnsvinya lpadsvmyvf drntkllqre ratqapdvkv
       61 ydyikdevgs rladrifdik kkfkkaldlg cgrghvskhi laesveelil sdmsptilqq
      121 vkttegikvl rtvmdeenfe fepdsldliv sslslhwvnd lprcfknink slkndgvfma
      181 aifggetlcs lqlaelerdg gisahispfa eirdigallt ranftmltid tdeivigyps
      241 mfelmwdlkg maennaarnr klrlnkdtil aaaaiykely gklkddgspy vpatfqviyl
      301 lgwkpdplqp kplergtgqv slkdlyrlde iiketkkvkt dedq
//
LOCUS       XP_034191388             321 aa            linear   INV 03-SEP-2025
DEFINITION  delta4-sphingolipid-FADS-like protein ifc [Osmia lignaria
            lignaria].
ACCESSION   XP_034191388
VERSION     XP_034191388.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335497.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191388.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="delta4-sphingolipid-FADS-like protein ifc"
                     /calculated_mol_wt=37161
     Region          6..42
                     /region_name="Lipid_DES"
                     /note="Sphingolipid Delta4-desaturase (DES); pfam08557"
                     /db_xref="CDD:462517"
     Region          26..315
                     /region_name="Delta4-sphingolipid-FADS-like"
                     /note="The Delta4-sphingolipid Fatty Acid Desaturase
                     (Delta4-sphingolipid-FADS)-like CD includes the
                     integral-membrane enzymes, dihydroceramide Delta-4
                     desaturase, involved in the synthesis of sphingosine; and
                     the human membrane fatty acid (lipid) desaturase...;
                     cd03508"
                     /db_xref="CDD:239585"
     Site            order(89,93,128,131..132,259,262..263)
                     /site_type="other"
                     /note="putative di-iron ligands [ion binding]"
                     /db_xref="CDD:239585"
     CDS             1..321
                     /gene="ifc"
                     /coded_by="XM_034335497.2:206..1171"
                     /db_xref="GeneID:117609319"
ORIGIN      
        1 mgqhvsrtdf ewvyteepha srrkiileky pqikklfgyd pnfkwivtgm vltqfisllf
       61 ikdlsypllf lmaycfggii nhslmlaihe iahnlafghs rpmanrlfgy fanlpigipv
      121 svsfkkyhli hhryqgdekl dtdlptllea klfcttfgkf cwiclqpffy afrplvvypm
      181 pptlleyinl iiqltfdgll wyflggkvlv yllagsamam glhpvaghfi sehymykkgf
      241 etysyygpln witfnvgyhn ehhdfpavpg srlpevkria pefydnlpqh nswvsvlydf
      301 vmdpdigpya rikrkhkgla s
//
LOCUS       XP_034191389             321 aa            linear   INV 03-SEP-2025
DEFINITION  delta4-sphingolipid-FADS-like protein ifc [Osmia lignaria
            lignaria].
ACCESSION   XP_034191389
VERSION     XP_034191389.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335498.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191389.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="delta4-sphingolipid-FADS-like protein ifc"
                     /calculated_mol_wt=37161
     Region          6..42
                     /region_name="Lipid_DES"
                     /note="Sphingolipid Delta4-desaturase (DES); pfam08557"
                     /db_xref="CDD:462517"
     Region          26..315
                     /region_name="Delta4-sphingolipid-FADS-like"
                     /note="The Delta4-sphingolipid Fatty Acid Desaturase
                     (Delta4-sphingolipid-FADS)-like CD includes the
                     integral-membrane enzymes, dihydroceramide Delta-4
                     desaturase, involved in the synthesis of sphingosine; and
                     the human membrane fatty acid (lipid) desaturase...;
                     cd03508"
                     /db_xref="CDD:239585"
     Site            order(89,93,128,131..132,259,262..263)
                     /site_type="other"
                     /note="putative di-iron ligands [ion binding]"
                     /db_xref="CDD:239585"
     CDS             1..321
                     /gene="ifc"
                     /coded_by="XM_034335498.2:163..1128"
                     /db_xref="GeneID:117609319"
ORIGIN      
        1 mgqhvsrtdf ewvyteepha srrkiileky pqikklfgyd pnfkwivtgm vltqfisllf
       61 ikdlsypllf lmaycfggii nhslmlaihe iahnlafghs rpmanrlfgy fanlpigipv
      121 svsfkkyhli hhryqgdekl dtdlptllea klfcttfgkf cwiclqpffy afrplvvypm
      181 pptlleyinl iiqltfdgll wyflggkvlv yllagsamam glhpvaghfi sehymykkgf
      241 etysyygpln witfnvgyhn ehhdfpavpg srlpevkria pefydnlpqh nswvsvlydf
      301 vmdpdigpya rikrkhkgla s
//
LOCUS       XP_034191390             321 aa            linear   INV 03-SEP-2025
DEFINITION  delta4-sphingolipid-FADS-like protein ifc [Osmia lignaria
            lignaria].
ACCESSION   XP_034191390
VERSION     XP_034191390.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335499.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191390.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="delta4-sphingolipid-FADS-like protein ifc"
                     /calculated_mol_wt=37161
     Region          6..42
                     /region_name="Lipid_DES"
                     /note="Sphingolipid Delta4-desaturase (DES); pfam08557"
                     /db_xref="CDD:462517"
     Region          26..315
                     /region_name="Delta4-sphingolipid-FADS-like"
                     /note="The Delta4-sphingolipid Fatty Acid Desaturase
                     (Delta4-sphingolipid-FADS)-like CD includes the
                     integral-membrane enzymes, dihydroceramide Delta-4
                     desaturase, involved in the synthesis of sphingosine; and
                     the human membrane fatty acid (lipid) desaturase...;
                     cd03508"
                     /db_xref="CDD:239585"
     Site            order(89,93,128,131..132,259,262..263)
                     /site_type="other"
                     /note="putative di-iron ligands [ion binding]"
                     /db_xref="CDD:239585"
     CDS             1..321
                     /gene="ifc"
                     /coded_by="XM_034335499.2:165..1130"
                     /db_xref="GeneID:117609319"
ORIGIN      
        1 mgqhvsrtdf ewvyteepha srrkiileky pqikklfgyd pnfkwivtgm vltqfisllf
       61 ikdlsypllf lmaycfggii nhslmlaihe iahnlafghs rpmanrlfgy fanlpigipv
      121 svsfkkyhli hhryqgdekl dtdlptllea klfcttfgkf cwiclqpffy afrplvvypm
      181 pptlleyinl iiqltfdgll wyflggkvlv yllagsamam glhpvaghfi sehymykkgf
      241 etysyygpln witfnvgyhn ehhdfpavpg srlpevkria pefydnlpqh nswvsvlydf
      301 vmdpdigpya rikrkhkgla s
//
LOCUS       XP_034191391             321 aa            linear   INV 03-SEP-2025
DEFINITION  delta4-sphingolipid-FADS-like protein ifc [Osmia lignaria
            lignaria].
ACCESSION   XP_034191391
VERSION     XP_034191391.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335500.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191391.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..321
                     /product="delta4-sphingolipid-FADS-like protein ifc"
                     /calculated_mol_wt=37161
     Region          6..42
                     /region_name="Lipid_DES"
                     /note="Sphingolipid Delta4-desaturase (DES); pfam08557"
                     /db_xref="CDD:462517"
     Region          26..315
                     /region_name="Delta4-sphingolipid-FADS-like"
                     /note="The Delta4-sphingolipid Fatty Acid Desaturase
                     (Delta4-sphingolipid-FADS)-like CD includes the
                     integral-membrane enzymes, dihydroceramide Delta-4
                     desaturase, involved in the synthesis of sphingosine; and
                     the human membrane fatty acid (lipid) desaturase...;
                     cd03508"
                     /db_xref="CDD:239585"
     Site            order(89,93,128,131..132,259,262..263)
                     /site_type="other"
                     /note="putative di-iron ligands [ion binding]"
                     /db_xref="CDD:239585"
     CDS             1..321
                     /gene="ifc"
                     /coded_by="XM_034335500.2:164..1129"
                     /db_xref="GeneID:117609319"
ORIGIN      
        1 mgqhvsrtdf ewvyteepha srrkiileky pqikklfgyd pnfkwivtgm vltqfisllf
       61 ikdlsypllf lmaycfggii nhslmlaihe iahnlafghs rpmanrlfgy fanlpigipv
      121 svsfkkyhli hhryqgdekl dtdlptllea klfcttfgkf cwiclqpffy afrplvvypm
      181 pptlleyinl iiqltfdgll wyflggkvlv yllagsamam glhpvaghfi sehymykkgf
      241 etysyygpln witfnvgyhn ehhdfpavpg srlpevkria pefydnlpqh nswvsvlydf
      301 vmdpdigpya rikrkhkgla s
//
LOCUS       XP_034191393             262 aa            linear   INV 03-SEP-2025
DEFINITION  hydroxypyruvate isomerase-like protein Gip [Osmia lignaria
            lignaria].
ACCESSION   XP_034191393
VERSION     XP_034191393.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335502.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191393.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..262
                     /product="hydroxypyruvate isomerase-like protein Gip"
                     /calculated_mol_wt=29635
     Region          3..259
                     /region_name="Hyi"
                     /note="Hydroxypyruvate/dehydroerythronate isomerase,
                     Hyi/OtnI family [Carbohydrate transport and metabolism];
                     COG3622"
                     /db_xref="CDD:442840"
     CDS             1..262
                     /gene="Gip"
                     /coded_by="XM_034335502.2:95..883"
                     /db_xref="GeneID:117609320"
ORIGIN      
        1 mslkfasnls fmfteapsit eryrlakeag fkavesgfpf gysvkdvsea rkkadieqil
       61 invytgdvtk gelgfaavpg qeenfkksid ttieyskaln clrihimsgk vnqisttnds
      121 tylnnllyav ekfekekiig viepinnitv pdyymnsfqk glelvktins pflklqldvf
      181 hlqhisgnit rnikelmpyi ghvqiaqvpn rhepdtpgei dykyvlatle qegyngyigl
      241 eyfpksssre glswiqkygy rl
//
LOCUS       XP_034191394             248 aa            linear   INV 03-SEP-2025
DEFINITION  SAP domain-containing ribonucleoprotein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191394
VERSION     XP_034191394.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335503.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191394.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..248
                     /product="SAP domain-containing ribonucleoprotein isoform
                     X1"
                     /calculated_mol_wt=27787
     Region          13..44
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     Region          14..>129
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     CDS             1..248
                     /gene="LOC117609321"
                     /coded_by="XM_034335503.2:385..1131"
                     /db_xref="GeneID:117609321"
ORIGIN      
        1 madssyekgl telskmkvad lkielkqrgl sttgnknelv erlqlaihgd salsldetae
       61 eildedavlg deeieelssk pdsqevekkr klstesnmna kkivlnrkpv ieevkneqte
      121 kaeidtktte vapaerkiik lselgvkerl emrakkfglp lseaakkevr sarfsinnqn
      181 nksaasvktp vhttyevlkk raerfgtsvs tlmekaelea riekrkarfg evkpanekvf
      241 hnkvkivk
//
LOCUS       XP_034191395             247 aa            linear   INV 03-SEP-2025
DEFINITION  SAP domain-containing ribonucleoprotein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191395
VERSION     XP_034191395.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335504.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191395.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..247
                     /product="SAP domain-containing ribonucleoprotein isoform
                     X2"
                     /calculated_mol_wt=27730
     Region          13..44
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     Region          14..>128
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     CDS             1..247
                     /gene="LOC117609321"
                     /coded_by="XM_034335504.2:387..1130"
                     /db_xref="GeneID:117609321"
ORIGIN      
        1 madssyekgl telskmkvad lkielkqrgl sttgnknelv erlqlaihds alsldetaee
       61 ildedavlgd eeieelsskp dsqevekkrk lstesnmnak kivlnrkpvi eevkneqtek
      121 aeidtkttev apaerkiikl selgvkerle mrakkfglpl seaakkevrs arfsinnqnn
      181 ksaasvktpv httyevlkkr aerfgtsvst lmekaelear iekrkarfge vkpanekvfh
      241 nkvkivk
//
LOCUS       XP_034191396             225 aa            linear   INV 03-SEP-2025
DEFINITION  SAP domain-containing ribonucleoprotein isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191396
VERSION     XP_034191396.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335505.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034191396.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..225
                     /product="SAP domain-containing ribonucleoprotein isoform
                     X3"
                     /calculated_mol_wt=25316
     Region          13..44
                     /region_name="SAP"
                     /note="SAP domain; pfam02037"
                     /db_xref="CDD:460424"
     Region          14..>129
                     /region_name="PLN03124"
                     /note="poly [ADP-ribose] polymerase; Provisional"
                     /db_xref="CDD:215591"
     CDS             1..225
                     /gene="LOC117609321"
                     /coded_by="XM_034335505.2:387..1064"
                     /db_xref="GeneID:117609321"
ORIGIN      
        1 madssyekgl telskmkvad lkielkqrgl sttgnknelv erlqlaihgd salsldetae
       61 eildedavlg deeieelssk pdsqevekkr klstesnmna kkivlnrkpv ieevkneqte
      121 kaeidtktte vapaerkiik lselgvkerl emrakkfglp lseaakkeht tyevlkkrae
      181 rfgtsvstlm ekaelearie krkarfgevk panekvfhnk vkivk
//
LOCUS       XP_034191397             171 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-fold modifier conjugating enzyme 1 isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191397
VERSION     XP_034191397.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335506.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..171
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..171
                     /product="ubiquitin-fold modifier conjugating enzyme 1
                     isoform X1"
                     /calculated_mol_wt=19683
     Region          8..158
                     /region_name="UBCc_UFC1"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-fold modifier-conjugating enzyme 1
                     (UFC1) and related proteins; cd11686"
                     /db_xref="CDD:467409"
     CDS             1..171
                     /gene="Ufc1"
                     /coded_by="XM_034335506.2:84..599"
                     /db_xref="GeneID:117609322"
ORIGIN      
        1 mmvdestkkt lsnipllqtk agprdkelwv qrlkeeyqal ikyvknnkds dndwfrlesn
       61 kegtrwfgkc wyihnflkye fevefdipvt ypttapeial peldgktakm yrggkicltd
      121 hfkplwarnv pkfgiahama lglgpwlave ipdliekgai thkdkinenk s
//
LOCUS       XP_034191398             137 aa            linear   INV 03-SEP-2025
DEFINITION  ubiquitin-fold modifier conjugating enzyme 1 isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_034191398
VERSION     XP_034191398.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335507.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..137
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..137
                     /product="ubiquitin-fold modifier conjugating enzyme 1
                     isoform X2"
                     /calculated_mol_wt=15715
     Region          <9..124
                     /region_name="UBCc_UFC1"
                     /note="Ubiquitin-conjugating enzyme E2, catalytic (UBCc)
                     domain of ubiquitin-fold modifier-conjugating enzyme 1
                     (UFC1) and related proteins; cd11686"
                     /db_xref="CDD:467409"
     CDS             1..137
                     /gene="Ufc1"
                     /coded_by="XM_034335507.2:116..529"
                     /db_xref="GeneID:117609322"
ORIGIN      
        1 mvdveeleyv knnkdsdndw frlesnkegt rwfgkcwyih nflkyefeve fdipvtyptt
       61 apeialpeld gktakmyrgg kicltdhfkp lwarnvpkfg iahamalglg pwlaveipdl
      121 iekgaithkd kinenks
//
LOCUS       XP_034191399              70 aa            linear   INV 03-SEP-2025
DEFINITION  guanine nucleotide-binding protein subunit gamma-1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191399
VERSION     XP_034191399.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335508.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..70
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..70
                     /product="guanine nucleotide-binding protein subunit
                     gamma-1"
                     /calculated_mol_wt=8047
     Region          8..65
                     /region_name="GGL"
                     /note="G protein gamma subunit-like motifs, the
                     alpha-helical G-gamma chain dimerizes with the G-beta
                     propeller subunit as part of the heterotrimeric G-protein
                     complex; involved in signal transduction via
                     G-protein-coupled receptors; cd00068"
                     /db_xref="CDD:238024"
     Site            order(8,11,15,18,22,27,29,32..33,36..37,40,49..50,61)
                     /site_type="other"
                     /note="beta subunit binding site [polypeptide binding]"
                     /db_xref="CDD:238024"
     CDS             1..70
                     /gene="Ggamma1"
                     /coded_by="XM_034335508.2:181..393"
                     /db_xref="GeneID:117609323"
ORIGIN      
        1 mdmvitnlqq qrqiteqlrr eaalkritvs kavedimkyi teheqedcll vgfssqksnp
       61 frekssctvf
//
LOCUS       XP_034183081            1510 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605644 [Osmia lignaria lignaria].
ACCESSION   XP_034183081
VERSION     XP_034183081.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327190.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183081.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1510
                     /product="uncharacterized protein LOC117605644"
                     /calculated_mol_wt=171933
     Region          <8..158
                     /region_name="GBP_C"
                     /note="Guanylate-binding protein, C-terminal domain;
                     pfam02841"
                     /db_xref="CDD:460721"
     Region          <631..1001
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..1510
                     /gene="LOC117605644"
                     /coded_by="XM_034327190.2:97..4629"
                     /db_xref="GeneID:117605644"
ORIGIN      
        1 mdnnlpdika seivqedlss kekltqsyll lkkkifgtde ltrkyndkqk ecerlkteld
       61 tttkvankat ynynstlakv iklellntky kknieslteq sneqkiktaa dqqhiqqlic
      121 kiedlkkned dkiiqydlek sslqekvkel eqelktlkks ydtkikkiek kiptehdnkp
      181 kvkeiginte ltrdeirqkp ktvekcvvtn etynvkedvy pvfcgkcevl leppphekic
      241 kmmtnscpkl iekisspkkv stplgrsrei nsnnykpenl ptlpntshlh nqnnhsnfip
      301 qnlltpstpv ssnppnycnn fmpplpnlms pgtyigstpv isvpltnlna inqnnqsesi
      361 aavasslsii sslhkkidtl etklemlskv kskrsnsccq hqhpttctyd mnssmqfnfm
      421 emwkrmadly enkdkkkerf dthkrnskkl nshkmnrltp kkrlqnthtn swkvesaakk
      481 vkhspsrkrl kkrkgryslf skvdissnns edlensnsds dtnsytdvfg nttisktnsn
      541 iesknspcte tsksiiisnn dlkattssth stdldeaeti etirdsvefs ksiggetdsg
      601 ilsdsvessk lmpleantnt fldsaeyent tkikssienr shpnknikpv vksklsnrps
      661 iktvlhltkf neltetntgl kppsqpnkcn eniedkeite aahdvitqdn actstatssv
      721 rkrkrkinef qeqkncnkra kllkklrslr knskcinnts qnisihqdal nhkdedsrss
      781 keqtnldhdt sitktedyvp kkrpriahvp ksttdhttsp nsslqvkkcl vknieerkie
      841 lqtisannev snttadhtkm qkyditlnel setnnelqet nddgtkqlks tsvniinssi
      901 edsvesnkde titpdiknvs seseiknspn vgihnynhsn itdesyvndl egvykcdhsn
      961 tiiesdvkds vnetmeteve snnqcevfqs leqkiensvq esecineqts ilfqqdlngc
     1021 qnfkekscde fdlhstlnks gdsmkildvp tpksdadtcn egvteecsin vqdsimkekn
     1081 tqrvkknist kkiksnkqck fievesmdpl qklrqyinkn ksqrtynqnk elsavrmltd
     1141 kfvkrqlqrl idndwgisvh cdvidklksv gssriiakei veflstiseg qpldkthtpp
     1201 aplmtknqqk ivtllvdlee wdpmifqlvl agieyklfrl nsdtvvtkta iesfarmyti
     1261 larikkdrek vrifccdaly clglnailvl ytvftcwpev fpnnetsnql lpkcmahfim
     1321 rqqgtdypkl nalknlvsvf ykypsetlsk dllnellttl qekshvevet aiillgkreg
     1381 ttwaykniir sallpmiinn klpseyrglc llgnlmrvfp ledkdnsvse tveqlcdlik
     1441 agseesseln egvisallsl srhkfdkvve ntltwkpakp lcdrtekqfn glfnqrslvf
     1501 wnkylrknkl
//
LOCUS       XP_034183082            1440 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605645 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183082
VERSION     XP_034183082.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327191.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183082.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1440
                     /product="uncharacterized protein LOC117605645 isoform X1"
                     /calculated_mol_wt=160966
     Region          <89..481
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <1033..1317
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1440
                     /gene="LOC117605645"
                     /coded_by="XM_034327191.2:229..4551"
                     /db_xref="GeneID:117605645"
ORIGIN      
        1 mststflgns dglvtglcpa pppppppppv pgqvpamrin tveaptikrp qiasndayep
       61 paaiqnamlt kdkkpftytp gmggkldlsq irsprmarrv aknandegie gppksalepk
      121 ptpapntgan lfvqpqvaip vfptnipiqp pinrtpptpp anktpptlpa nktppitpek
      181 qaepikvvtk vdtkatpnaa tttqpsspes pstptqvtlt kaptpwlqnk nkpqeelpew
      241 akrssvnkqs sspesptspp vyasvqqtqp qqqpvqwqqt qqkqqpiqqr pqqqhqqpqq
      301 fnqqqqkrpy ssppltqqts pqanlqervi piriedrpsv fdvkhesghh qfkqptphhq
      361 qrwggpvqnq fqnqvqspqp qpssggtfii pmmvegserk pvggpvsnts ypqpivrnnt
      421 pnienqtvkv ipqrgpvqiq qpdsgpvqsr sfrvlqkitd tdsndvdteq vrklqlsddd
      481 rilmnkfkeq vdnesslhne edpryrgaai psrafrflqt mtdsgdaavm ntapraqsai
      541 nkkqnrnskt feetqanlpp seqqvpepkk ymgsaipsrs frilqamtap esvatqenrq
      601 adytcqtenn vpgnqqggly lppcpppfws pdngwwgyyp lpypavanes fpyhsdntay
      661 vyfpiynqny pgyydsvnaa kngypsvyas yvvpvspptn ncnlnyeqvg ngvavpkidp
      721 qlnipdneth egaiskdtdd lrgppqwdek hvsrspssns kdsdcttamn itdeeeipev
      781 mvvsepdanq sdrsekactn gslnvsvpny tyidtsdssd tsdsqsdnes ivestksssd
      841 nnandtssds ddsdsylays tglnphgrfd dnknpmncpd kecnthvqev esrnntdcds
      901 ddgqleghle etgtnafphk lsviyedver pdsvsprppe gitpfeatdd ppdeadtttv
      961 svslplrfkf svsennedvt tvivgdsmik perpckdegr ssktqehknh tdkinevsan
     1021 flvnndtsad ftvkrdsses krraksnmne rtngnkvekn ddpvvphvnf tlrkiptrfn
     1081 rinceetvet eftikpkkas lkkeedrsnk erkervnntn ndeepsnked npsyieetnr
     1141 ethdfnrnil kpevivsecn wtedpsketi nsqstnldie qnevrtedns nvpdshknee
     1201 dveekgkevk hllsvqnsre etddedsgvt sdmsrmisev dtdsectssr nirkyqrtqt
     1261 hsrlfrllnd dsilpegtek sndtpcrrky lslplnntfn ydenycsnys sgitspeysp
     1321 vceqswrrlh dtensrvpdq nghghpltrq ervsckedpy yqawktsktp insdhsdvmp
     1381 slafkvlesr rplwsyrvnv lcprikstks vpqvlrtnna tdnplpsnps pyvnlksdhc
//
LOCUS       XP_034183083            1399 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605645 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183083
VERSION     XP_034183083.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327192.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183083.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1399
                     /product="uncharacterized protein LOC117605645 isoform X2"
                     /calculated_mol_wt=156909
     Region          <48..440
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     Region          <992..1276
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1399
                     /gene="LOC117605645"
                     /coded_by="XM_034327192.2:143..4342"
                     /db_xref="GeneID:117605645"
ORIGIN      
        1 mmaptikrpq iasndayepp aaiqnamltk dkkpftytpg mggkldlsqi rsprmarrva
       61 knandegieg ppksalepkp tpapntganl fvqpqvaipv fptnipiqpp inrtpptppa
      121 nktpptlpan ktppitpekq aepikvvtkv dtkatpnaat ttqpsspesp stptqvtltk
      181 aptpwlqnkn kpqeelpewa krssvnkqss spesptsppv yasvqqtqpq qqpvqwqqtq
      241 qkqqpiqqrp qqqhqqpqqf nqqqqkrpys sppltqqtsp qanlqervip iriedrpsvf
      301 dvkhesghhq fkqptphhqq rwggpvqnqf qnqvqspqpq pssggtfiip mmvegserkp
      361 vggpvsntsy pqpivrnntp nienqtvkvi pqrgpvqiqq pdsgpvqsrs frvlqkitdt
      421 dsndvdteqv rklqlsdddr ilmnkfkeqv dnesslhnee dpryrgaaip srafrflqtm
      481 tdsgdaavmn tapraqsain kkqnrnsktf eetqanlpps eqqvpepkky mgsaipsrsf
      541 rilqamtape svatqenrqa dytcqtennv pgnqqgglyl ppcpppfwsp dngwwgyypl
      601 pypavanesf pyhsdntayv yfpiynqnyp gyydsvnaak ngypsvyasy vvpvspptnn
      661 cnlnyeqvgn gvavpkidpq lnipdnethe gaiskdtddl rgppqwdekh vsrspssnsk
      721 dsdcttamni tdeeeipevm vvsepdanqs drsekactng slnvsvpnyt yidtsdssdt
      781 sdsqsdnesi vestksssdn nandtssdsd dsdsylayst glnphgrfdd nknpmncpdk
      841 ecnthvqeve srnntdcdsd dgqleghlee tgtnafphkl sviyedverp dsvsprppeg
      901 itpfeatddp pdeadtttvs vslplrfkfs vsennedvtt vivgdsmikp erpckdegrs
      961 sktqehknht dkinevsanf lvnndtsadf tvkrdssesk rraksnmner tngnkveknd
     1021 dpvvphvnft lrkiptrfnr inceetvete ftikpkkasl kkeedrsnke rkervnntnn
     1081 deepsnkedn psyieetnre thdfnrnilk pevivsecnw tedpsketin sqstnldieq
     1141 nevrtednsn vpdshkneed veekgkevkh llsvqnsree tddedsgvts dmsrmisevd
     1201 tdsectssrn irkyqrtqth srlfrllndd silpegteks ndtpcrrkyl slplnntfny
     1261 denycsnyss gitspeyspv ceqswrrlhd tensrvpdqn ghghpltrqe rvsckedpyy
     1321 qawktsktpi nsdhsdvmps lafkvlesrr plwsyrvnvl cprikstksv pqvlrtnnat
     1381 dnplpsnpsp yvnlksdhc
//
LOCUS       XP_076548209             897 aa            linear   INV 03-SEP-2025
DEFINITION  ionotropic receptor 8a [Osmia lignaria lignaria].
ACCESSION   XP_076548209
VERSION     XP_076548209.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692094.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..897
                     /product="ionotropic receptor 8a"
                     /calculated_mol_wt=101767
     Region          381..784
                     /region_name="PBP2_iGluR_putative"
                     /note="The ligand-binding domain of putative ionotropic
                     glutamate receptors, a member of the type 2 periplasmic
                     binding fold protein superfamily; cd13717"
                     /db_xref="CDD:270435"
     Site            order(414,426,430,476..477,480,485..488,745,763..764,
                     767..772,774,777)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:270435"
     Site            order(445,472..474,479,638,641..643,722,748)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270435"
     CDS             1..897
                     /gene="Ir8a"
                     /coded_by="XM_076692094.1:2766..5459"
                     /db_xref="GeneID:117605646"
ORIGIN      
        1 mstslllasa ffyasflgfs lcqtsmtmli vleeadtqil gllneaapqa eknfgedvit
       61 veistvqidr tnleasferv caalfkgisi vldmtwtgwe klrnmadkng iiykrgdssi
      121 npyvqaiddl lmlknatdvg lifedereln qslyyligns iirlvvidef tektvskirs
      181 mrpspsyyai yastskmedl frtaiqgglv krdgiwnlvf tdnnykdfky ingdlqlnts
      241 itvltmkknv ccrlmgessc ncptdvaifs nyfkrlvglv vslmselqks gisvepktgr
      301 csspnasqta snvtseafnk nilarlggnd tfeywsekam itykaeiefe tlnngvmepl
      361 atwtrhtkik eaegrkiepa rrffrigtnp lvpwtvakvd patgqvlkde ngneiwegyc
      421 idfvkklsee mqfdydlvvp qdrqfgkklp ngqwngligd lakgetdivi aaltmtsere
      481 eiidfvapyf eqsgilivmr kpvrkpslfk fmtvlkvevw lsivgaltlt gimiwildky
      541 spysarnnkr lypypcreft lkesfwfalt sftpqgggea pkalssrtlv aaywlfvvlm
      601 latftanlaa fltvermqsp vqsleqlarq srinytvlan ssvhqyfmnm knaedklytv
      661 wkeitlnsts dqveyrvwdy pikeqyghil qaitqvgpvk sseegfrkvi esenaefafi
      721 hdsseiryea trncnltevg dvfaeqpyai avqqgshlqe eisrrildlq kdryfetlss
      781 kywnqslkaq cmnsddnegi tleslggvfi atlfglalam itlageilyy rkrnpqkdkr
      841 knkkkvntid ndkimiqkla sklqmkpapn tmlfekqmna prvshisvyp rnfsfke
//
LOCUS       XP_034183085             851 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605647 [Osmia lignaria lignaria].
ACCESSION   XP_034183085
VERSION     XP_034183085.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327194.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183085.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..851
                     /product="uncharacterized protein LOC117605647"
                     /calculated_mol_wt=91108
     Region          182..238
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..851
                     /gene="LOC117605647"
                     /coded_by="XM_034327194.2:619..3174"
                     /db_xref="GeneID:117605647"
ORIGIN      
        1 mrgkqqalsq hqqnpeesaq lgssgnvqqp gghletlvhv gkevagvktr pttpvlandg
       61 gggfwlatva agagapaglp phgthhptmd ptcgsaetgf insqpsmaef mtalpqitgd
      121 gslqhspgtg gsispgshhp gyhtmmdphg vadpsvnvpe ypwmkekktt rksnqqengl
      181 prrlrtaytn tqllelekef hfnkylcrpr rieiaasldl terqvkvwfq nrrmkhkrqt
      241 lskedgdekd gstsdgmeks ksekmlaids eekkschncd istvsdvgst lsgdvtelnp
      301 sgrrssnsnn nntptatnnn snpgfnansn gassvgstns vsssfekmia dddsrsqdgs
      361 evtspsmtak klpsdvrvkv esghkspnpa kqqisvskks psantkelca vtsngvllam
      421 pdstvstpvl pgqnstrslt psstpgtpvs vqlqqgsplg iqathaaymq rprsspssss
      481 svaapmmhgs tnagtmspha srnitsnqqt hfqqqtvyqs tsavdyrngv ptrqtvpaat
      541 qqtpsqnnyc srdtyqqpri sypgevhply arqnqvlgsr vghhvrattg tasrtmynas
      601 mqfhqqqsqy antsgeyngy pqagppyhas yhrgmqsnna ygagqtytaq heqttesyma
      661 pgtygypstg nyhganenla shghaavsvq tgqhyyndiq qqqqhpqqht aegyvthqpp
      721 mhpsstdyra gnkchpnayy eqtsqlhvhq ggeasnipns yvsspdpfpa pgmtttatai
      781 aaataavitp pgsaaqadsn ndsygnygnf ygepvhtaap ppsadnsnss sdfnfltnla
      841 ndfapeyyql s
//
LOCUS       XP_034183088             551 aa            linear   INV 03-SEP-2025
DEFINITION  heparan-alpha-glucosaminide N-acetyltransferase [Osmia lignaria
            lignaria].
ACCESSION   XP_034183088
VERSION     XP_034183088.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327197.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183088.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..551
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /calculated_mol_wt=61925
     Region          168..549
                     /region_name="COG4299"
                     /note="Predicted acyltransferase, DUF1624 domain [General
                     function prediction only]"
                     /db_xref="CDD:443440"
     CDS             1..551
                     /gene="LOC117605649"
                     /coded_by="XM_034327197.2:353..2008"
                     /db_xref="GeneID:117605649"
ORIGIN      
        1 mdewfcdret lkydeacinl rtdhedsnvw lyllstdcal cpytrikqii anstssviid
       61 tvrpttfrif davdpdefvt akntsnvice ltpnlgqfgi yeltvqnrsc dikvlnepts
      121 pyteffvilg vlvlflfgls glkslwhacr kkctrrqvdd tamrqpvkrr vkaidtvrga
      181 stllmifvnd gsggyrtlgh atwnglllgd lvfpcfiwim gvcipialgs qlkrmvpkhv
      241 ilygivkrsi llfligvsln tvstgpqlen irifgvlqrf gvtyfmvslt ylflmlkrpt
      301 kspmlrdvqd fflllpqccv mavivavhci vtfclpvpgc ptgylgpggl hddakyfdcv
      361 ggaagyidrl vlkeqhlhhs mtvyqsgpfd pegilgclts tfhvflglha giimmsykdw
      421 kervvrwlaw aalfgcigca lhftnvipvn kklwslsfvf vttsfalafl sacyllvdvl
      481 kvwkggpfri pgmnglllyv ghmtcyqnfp fhwsigsmns rglrlceaiw gvalwtiiay
      541 imhkkrtyis l
//
LOCUS       XP_034183089             482 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605650 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183089
VERSION     XP_034183089.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327198.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..482
                     /product="uncharacterized protein LOC117605650 isoform X1"
                     /calculated_mol_wt=52768
     Region          370..423
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..482
                     /gene="LOC117605650"
                     /coded_by="XM_034327198.2:627..2075"
                     /db_xref="GeneID:117605650"
ORIGIN      
        1 mmmmdmgmyg tygkpdsypn yvctgqgnhh hhqpvsvggh vpvppspela pahyfpqtav
       61 spyssssses flaaesaass gtppqgfysp sagvlhedgt ttaiisseng lsytnldyat
      121 ssssgyptgq qhqhpngshg svdphqsyhv qqaayrdehh hhhlpgstsg lhqtsmvppa
      181 hrteheqqlh hqssshhhhh hhhhhepdyq lpvagttnyl hqlpttedtl hyhpqirqnd
      241 ytahttghyk eenpdptayh vlqqsghpqh phqhahghhh qqhphghhpp lpqqqqqqqp
      301 hvptykwmqv krnvpkpvaa ksnssvgdyg gssigggnas aytntangpv scltggplvg
      361 iaggfnntgr tnftnkqlte lekefhfnky ltrarrieia salqlnetqv kiwfqnrrmk
      421 qkkrmkegli psenttvapl ggarsssnsp tnsstheass glglsnftnd nsresppsss
      481 kd
//
LOCUS       XP_034183090             481 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605650 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183090
VERSION     XP_034183090.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327199.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..481
                     /product="uncharacterized protein LOC117605650 isoform X2"
                     /calculated_mol_wt=52696
     Region          369..422
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..481
                     /gene="LOC117605650"
                     /coded_by="XM_034327199.2:629..2074"
                     /db_xref="GeneID:117605650"
ORIGIN      
        1 mmmmdmgmyg tygkpdsypn yvctgqgnhh hhqpvsvggh vpvppspela pahyfpqtav
       61 spyssssses flaaesaass gtppqgfysp sagvlhedgt ttaiisseng lsytnldyat
      121 ssssgyptgq qhqhpngshg svdphqsyhv qqaayrdehh hhhlpgstsg lhqtsmvppa
      181 hrteheqqlh hqssshhhhh hhhhhepdyq lpvagttnyl hqlpttedtl hyhpqirqnd
      241 ytahttghyk eenpdptayh vlqqsghpqh phqhahghhh qqhphghhpp lpqqqqqqqp
      301 hvptykwmqv krnvpkpvak snssvgdygg ssigggnasa ytntangpvs cltggplvgi
      361 aggfnntgrt nftnkqltel ekefhfnkyl trarrieias alqlnetqvk iwfqnrrmkq
      421 kkrmkeglip senttvaplg garsssnspt nsstheassg lglsnftndn sresppsssk
      481 d
//
LOCUS       XP_034183091             458 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605651 [Osmia lignaria lignaria].
ACCESSION   XP_034183091
VERSION     XP_034183091.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327200.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..458
                     /product="uncharacterized protein LOC117605651"
                     /calculated_mol_wt=50316
     CDS             1..458
                     /gene="LOC117605651"
                     /coded_by="XM_034327200.2:1679..3055"
                     /db_xref="GeneID:117605651"
ORIGIN      
        1 mpiaggilva gtaahclssa agmlrflarl sgrrrhvrrs cgpgggarav eetveaagvg
       61 devadegrge grgvvgceav gaastieevs spvadgevcc gdssttnyhh rrhhlhhihr
      121 rhshhrhqcq qrhrchqrrd svasnelvvd eeaddevtcq dqtatdaaaa asasaaaahp
      181 shrdihqfhh lhhhphlrdl hqrlqddrth rdredtndri vrtsehhsdg rtttdnpatv
      241 tamtrgcrip pylatllils ytlfgiadac ssrstpkprp ptptprpnit fhmytcppdy
      301 aewyclngat cftvkivdsl lynclcangy igqrcefkdl dgsylpsrqr vmletasiag
      361 gatiavflvv iiciaayihc krkqkelrss ncvdtvdgpg rdpelrpfsn rnrslmifmt
      421 knpnssaaie qtrmpgwncp evesmrmasi segkrsnq
//
LOCUS       XP_076548210             413 aa            linear   INV 03-SEP-2025
DEFINITION  apidaecin 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548210
VERSION     XP_076548210.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692095.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..413
                     /product="apidaecin 1 isoform X1"
                     /calculated_mol_wt=47090
     CDS             1..413
                     /gene="Apid1"
                     /coded_by="XM_076692095.1:179..1420"
                     /db_xref="GeneID:117605654"
ORIGIN      
        1 mktifiltil giafattvlg eealessylr vpreakpepe pepgtkplyi prpppqphpr
       61 lrreadpepe pasrpvyvpp prpphprlrr eadpepepas rpvyvppprp phprlrread
      121 pepepasrpv yvppprpphp rlrreadpep epasrpvyvp pprpphprlr readpepepa
      181 srpvyvpppr pphprlrrea dpepepasrp vyvppprpph prlrreadpe pepasrpvyv
      241 ppprpphprl rreadpepep asrpvyvppp rpphprlrre adpepepasr pvyvppprpp
      301 hprlrreadp epepasrpvy vppprpphpr lrreadpepe pasrpvyvpp prpphprlrr
      361 eadpepepas rpvyvppprp phprlrread pepepgtkpi ymprpppphp rlr
//
LOCUS       XP_076548211             386 aa            linear   INV 03-SEP-2025
DEFINITION  apidaecin 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548211
VERSION     XP_076548211.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692096.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..386
                     /product="apidaecin 1 isoform X2"
                     /calculated_mol_wt=43994
     CDS             1..386
                     /gene="Apid1"
                     /coded_by="XM_076692096.1:180..1340"
                     /db_xref="GeneID:117605654"
ORIGIN      
        1 mktifiltil giafattvlg eealessylr vpreakpepe pepgtkplyi prpppqphpr
       61 lrreadpepe pasrpvyvpp prpphprlrr eadpepepas rpvyvppprp phprlrread
      121 pepepasrpv yvppprpphp rlrreadpep epasrpvyvp pprpphprlr readpepepa
      181 srpvyvpppr pphprlrrea dpepepasrp vyvppprpph prlrreadpe pepasrpvyv
      241 ppprpphprl rreadpepep asrpvyvppp rpphprlrre adpepepasr pvyvppprpp
      301 hprlrreadp epepasrpvy vppprpphpr lrreadpepe pasrpvyvpp prpphprlrr
      361 eadpepepgt kpiymprppp phprlr
//
LOCUS       XP_034183092             381 aa            linear   INV 03-SEP-2025
DEFINITION  protein AAR2 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034183092
VERSION     XP_034183092.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327201.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..381
                     /product="protein AAR2 homolog"
                     /calculated_mol_wt=43253
     Region          20..364
                     /region_name="AAR2"
                     /note="AAR2 protein; pfam05282"
                     /db_xref="CDD:461609"
     CDS             1..381
                     /gene="LOC117605652"
                     /coded_by="XM_034327201.2:360..1505"
                     /db_xref="GeneID:117605652"
ORIGIN      
        1 masessiemd qtlaqrllve gavlvvlnvp igtefgidlk swntadhfkg ikmippgfhy
       61 vhysavnefg eatpkigflh ifkkseflvk qwdakeeels sedvpeetvg rlkdhlkdld
      121 rflgcypydv wkqwkeltnh itsavvercs picgfvrsal elehcsdalr prggtsspkr
      181 rrtggltiee kekellpdmk prpgtelrls eipdkhypdg aspteitkyc ldasyaldsv
      241 lnklalpmei igemqlafvc flvgqsldaf ehwkklvsli cgadcaisqr rsiyfefmks
      301 leiqlthvpe dilcdivvnn nfiyqslrkl fanieinsel dgrlksyasr frerlqtkfl
      361 wnfsnlqdea eddapvivsl e
//
LOCUS       XP_034183096             360 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605653 [Osmia lignaria lignaria].
ACCESSION   XP_034183096
VERSION     XP_034183096.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327205.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183096.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..360
                     /product="uncharacterized protein LOC117605653"
                     /calculated_mol_wt=40223
     Region          <129..>339
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..360
                     /gene="LOC117605653"
                     /coded_by="XM_034327205.2:145..1227"
                     /db_xref="GeneID:117605653"
ORIGIN      
        1 msnneplqpa vnqeplafel mksdqndntk napaenaalp aveepnadkr ctestapsnt
       61 ndqaievatk eaeisnensk avpeaanest getsekvvts eisevqqlpa vpevqqqtig
      121 agdtcalpqr denantqktq eeiaaatvnn rtpqsdekss medkkenede lavsklkeef
      181 qkaseerdsy qkklkgtekk lallqasyda lmkgegdevm lrrmvdqlkn kliqtslqle
      241 drirvvanqe kqisalnsqv aslkeveslt rsllqirnme vkhlqaevdd mevrineerd
      301 ryntminkmd aavklnadlk teyetqlglf rdlrekyeek vtllseekra lennaqsvpk
//
LOCUS       XP_034183103             162 aa            linear   INV 03-SEP-2025
DEFINITION  mitochondrial import inner membrane translocase subunit Tim17-B
            [Osmia lignaria lignaria].
ACCESSION   XP_034183103
VERSION     XP_034183103.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327212.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..162
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..162
                     /product="mitochondrial import inner membrane translocase
                     subunit Tim17-B"
                     /calculated_mol_wt=16894
     Region          1..161
                     /region_name="TIM22"
                     /note="Mitochondrial import inner membrane translocase,
                     subunit TIM22 [Posttranslational modification, protein
                     turnover, chaperones]; cl02381"
                     /db_xref="CDD:470560"
     CDS             1..162
                     /gene="LOC117605655"
                     /coded_by="XM_034327212.2:1..489"
                     /db_xref="GeneID:117605655"
ORIGIN      
        1 meeyarepcp wrivddcgga ftmgaiggav fqsikgfrna psginkrllg slmaikqrsp
       61 iiagnfalwg gmfstidctl vhlrkkedpw nsiisgaatg gilaarnglp amagsaiigg
      121 vllaliegvg ifitrlsadq fkppsftedp thlgqasqgh ps
//
LOCUS       XP_034183107             104 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605657 [Osmia lignaria lignaria].
ACCESSION   XP_034183107
VERSION     XP_034183107.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327216.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..104
                     /product="uncharacterized protein LOC117605657"
                     /calculated_mol_wt=12014
     Region          16..89
                     /region_name="SNAPC5"
                     /note="snRNA-activating protein complex subunit 19, SNAPc
                     subunit 19; pfam15497"
                     /db_xref="CDD:464748"
     CDS             1..104
                     /gene="LOC117605657"
                     /coded_by="XM_034327216.2:175..489"
                     /db_xref="GeneID:117605657"
ORIGIN      
        1 meniekysar vntnellayq rdlkeekell qnmlikidkq ihglqveqlh llglmnkslr
       61 gaeqpmshks vddnqedltn nsldlsvatt fkhyeeemed eddi
//
LOCUS       XP_034183108             104 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605657 [Osmia lignaria lignaria].
ACCESSION   XP_034183108
VERSION     XP_034183108.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327217.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..104
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..104
                     /product="uncharacterized protein LOC117605657"
                     /calculated_mol_wt=12014
     Region          16..89
                     /region_name="SNAPC5"
                     /note="snRNA-activating protein complex subunit 19, SNAPc
                     subunit 19; pfam15497"
                     /db_xref="CDD:464748"
     CDS             1..104
                     /gene="LOC117605657"
                     /coded_by="XM_034327217.2:158..472"
                     /db_xref="GeneID:117605657"
ORIGIN      
        1 meniekysar vntnellayq rdlkeekell qnmlikidkq ihglqveqlh llglmnkslr
       61 gaeqpmshks vddnqedltn nsldlsvatt fkhyeeemed eddi
//
LOCUS       XP_034190785            1404 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase 4D interacting protein centrosomin isoform X1
            [Osmia lignaria lignaria].
ACCESSION   XP_034190785
VERSION     XP_034190785.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334894.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190785.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1404
                     /product="phosphodiesterase 4D interacting protein
                     centrosomin isoform X1"
                     /calculated_mol_wt=162971
     Region          88..>730
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          442..>1209
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1404
                     /gene="cnn"
                     /coded_by="XM_034334894.2:761..4975"
                     /db_xref="GeneID:117609054"
ORIGIN      
        1 mrnnyrnmff agygysnqhq nnppsptrts awgsvyspfr ntnlplqdit msqtlpling
       61 tnvkspgkts ptgvigtrdg ggigrtmkey edqlealkke nfnlklriyf leermgitsa
      121 denaikknie lkveieslrk ellekqells qaakafelie eqkeassrnq aqyqqslene
      181 rekirlleke laeyqekvad asiyykeafg itpekalene eklhqmeelv asleaevkqv
      241 tnsleeervw aqeleserde frerleaetr lkenlaaerv qdieelrekv keleeqvlkr
      301 dtvvqqsrne liekeriike knaqldekyr ayeelnavse krkkqvdqlr asiktrddal
      361 tdlnnkhral ltqfengyak rsppsspsam npiedplqsr mgqkltcvqg mtkkantcld
      421 wepnrerstr mksplqtlsg evkdvrdlik eieekdadlk rqeesrkqlv lklcnmqkhv
      481 ettdyrlkkl egehekaikt iqgfmerqqq lenvklrkeq kimeleieln rlreceniqs
      541 srdshnqfir rdfstdmtdd perdpsnqqr fdemeakind lrdqietika eksrlekqie
      601 veseelqgrl qdkeqkievl eiekqtireq lqdkekeldk lketaktege evleredllr
      661 qlqqrdteie eknqkieqls kelqvktqnl qklvntelws knkeiaklhn hmtatycher
      721 srnksdmmqe sasaqlstlv kelndigirv tftneviqln yvdgnepidv ktmtdyvqkl
      781 vmqkneleke vdylkwlklv srpdiateid gygdnqtera kkycellrth lkdlmkfmqe
      841 mmktvdhadt vgnehkrivl dvlmsskils ddfvnalegl stndlpfnle qvsarsmdns
      901 vkkswsenll satknhgstq sdseafsepd rtvslarigl qetqqkslnr srfskytktf
      961 sdsedsleyv pyhniyqndl ndsetshhiq elketnally nelsalrndl tsnvsfdcef
     1021 dekispliik leksqkfcek lqsslerrth elhvlkkenk qncarkaqle kklvdlesma
     1081 aemnkqkael mhykenserk taemlitlnr endalrtrik kleeenetak asisaltkel
     1141 dhltlshsqi lventkltnd klrleqemrk tesrcdmtvr sihdkfnkei sdlnqvnesh
     1201 rvrlqeleva nkelrrhval ceasdsapss sgvssiptea ilkqtcedfm qeypaysnsq
     1261 ywlpvsyptm ggrsktscsp dlgiesdaai ttmrplkdtl kitesmtnll sdedngnsnr
     1321 avremdnesp lpiegldeve alkqenetlk rrlmktrral edtfqhlsas nknkknveka
     1381 iakqlqitks ilkktrtyee pldn
//
LOCUS       XP_034190787            1380 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase 4D interacting protein centrosomin isoform X2
            [Osmia lignaria lignaria].
ACCESSION   XP_034190787
VERSION     XP_034190787.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334896.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190787.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1380
                     /product="phosphodiesterase 4D interacting protein
                     centrosomin isoform X2"
                     /calculated_mol_wt=160048
     Region          64..>706
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          418..>1185
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1380
                     /gene="cnn"
                     /coded_by="XM_034334896.2:109..4251"
                     /db_xref="GeneID:117609054"
ORIGIN      
        1 mgeflnetaa tvnyfdatnl plqditmsqt lplingtnvk spgktsptgv igtrdgggig
       61 rtmkeyedql ealkkenfnl klriyfleer mgitsadena ikknielkve ieslrkelle
      121 kqellsqaak afelieeqke assrnqaqyq qslenereki rllekelaey qekvadasiy
      181 ykeafgitpe kaleneeklh qmeelvasle aevkqvtnsl eeervwaqel eserdefrer
      241 leaetrlken laaervqdie elrekvkele eqvlkrdtvv qqsrneliek eriikeknaq
      301 ldekyrayee lnavsekrkk qvdqlrasik trddaltdln nkhralltqf engyakrspp
      361 sspsamnpie dplqsrmgqk ltcvqgmtkk antcldwepn rerstrmksp lqtlsgevkd
      421 vrdlikeiee kdadlkrqee srkqlvlklc nmqkhvettd yrlkklegeh ekaiktiqgf
      481 merqqqlenv klrkeqkime leielnrlre ceniqssrds hnqfirrdfs tdmtddperd
      541 psnqqrfdem eakindlrdq ietikaeksr lekqievese elqgrlqdke qkievleiek
      601 qtireqlqdk ekeldklket aktegeevle redllrqlqq rdteieeknq kieqlskelq
      661 vktqnlqklv ntelwsknke iaklhnhmta tychersrnk sdmmqesasa qlstlvkeln
      721 digirvtftn eviqlnyvdg nepidvktmt dyvqklvmqk nelekevdyl kwlklvsrpd
      781 iateidgygd nqterakkyc ellrthlkdl mkfmqemmkt vdhadtvgne hkrivldvlm
      841 sskilsddfv naleglstnd lpfnleqvsa rsmdnsvkks wsenllsatk nhgstqsdse
      901 afsepdrtvs lariglqetq qkslnrsrfs kytktfsdse dsleyvpyhn iyqndlndse
      961 tshhiqelke tnallynels alrndltsnv sfdcefdeki spliikleks qkfceklqss
     1021 lerrthelhv lkkenkqnca rkaqlekklv dlesmaaemn kqkaelmhyk enserktaem
     1081 litlnrenda lrtrikklee enetakasis altkeldhlt lshsqilven tkltndklrl
     1141 eqemrktesr cdmtvrsihd kfnkeisdln qvneshrvrl qelevankel rrhvalceas
     1201 dsapsssgvs sipteailkq tcedfmqeyp aysnsqywlp vsyptmggrs ktscspdlgi
     1261 esdaaittmr plkdtlkite smtnllsded ngnsnravre mdnesplpie gldevealkq
     1321 enetlkrrlm ktrraledtf qhlsasnknk knvekaiakq lqitksilkk trtyeepldn
//
LOCUS       XP_034190788            1373 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase 4D interacting protein centrosomin isoform X3
            [Osmia lignaria lignaria].
ACCESSION   XP_034190788
VERSION     XP_034190788.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334897.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190788.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1373
                     /product="phosphodiesterase 4D interacting protein
                     centrosomin isoform X3"
                     /calculated_mol_wt=159324
     Region          57..>699
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          411..>1178
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1373
                     /gene="cnn"
                     /coded_by="XM_034334897.2:160..4281"
                     /db_xref="GeneID:117609054"
ORIGIN      
        1 mgcgrsrlna stlleniqdk pikiqvmfgt nvkspgktsp tgvigtrdgg gigrtmkeye
       61 dqlealkken fnlklriyfl eermgitsad enaikkniel kveieslrke llekqellsq
      121 aakafeliee qkeassrnqa qyqqslener ekirllekel aeyqekvada siyykeafgi
      181 tpekalenee klhqmeelva sleaevkqvt nsleeervwa qeleserdef rerleaetrl
      241 kenlaaervq dieelrekvk eleeqvlkrd tvvqqsrnel iekeriikek naqldekyra
      301 yeelnavsek rkkqvdqlra siktrddalt dlnnkhrall tqfengyakr sppsspsamn
      361 piedplqsrm gqkltcvqgm tkkantcldw epnrerstrm ksplqtlsge vkdvrdlike
      421 ieekdadlkr qeesrkqlvl klcnmqkhve ttdyrlkkle gehekaikti qgfmerqqql
      481 envklrkeqk imeleielnr lreceniqss rdshnqfirr dfstdmtddp erdpsnqqrf
      541 demeakindl rdqietikae ksrlekqiev eseelqgrlq dkeqkievle iekqtireql
      601 qdkekeldkl ketaktegee vleredllrq lqqrdteiee knqkieqlsk elqvktqnlq
      661 klvntelwsk nkeiaklhnh mtatychers rnksdmmqes asaqlstlvk elndigirvt
      721 ftneviqlny vdgnepidvk tmtdyvqklv mqknelekev dylkwlklvs rpdiateidg
      781 ygdnqterak kycellrthl kdlmkfmqem mktvdhadtv gnehkrivld vlmsskilsd
      841 dfvnalegls tndlpfnleq vsarsmdnsv kkswsenlls atknhgstqs dseafsepdr
      901 tvslariglq etqqkslnrs rfskytktfs dsedsleyvp yhniyqndln dsetshhiqe
      961 lketnallyn elsalrndlt snvsfdcefd ekispliikl eksqkfcekl qsslerrthe
     1021 lhvlkkenkq ncarkaqlek klvdlesmaa emnkqkaelm hykenserkt aemlitlnre
     1081 ndalrtrikk leeenetaka sisaltkeld hltlshsqil ventkltndk lrleqemrkt
     1141 esrcdmtvrs ihdkfnkeis dlnqvneshr vrlqelevan kelrrhvalc easdsapsss
     1201 gvssipteai lkqtcedfmq eypaysnsqy wlpvsyptmg grsktscspd lgiesdaait
     1261 tmrplkdtlk itesmtnlls dedngnsnra vremdnespl piegldevea lkqenetlkr
     1321 rlmktrrale dtfqhlsasn knkknvekai akqlqitksi lkktrtyeep ldn
//
LOCUS       XP_034190789            1347 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase 4D interacting protein centrosomin isoform X4
            [Osmia lignaria lignaria].
ACCESSION   XP_034190789
VERSION     XP_034190789.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334898.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190789.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1347
                     /product="phosphodiesterase 4D interacting protein
                     centrosomin isoform X4"
                     /calculated_mol_wt=156469
     Region          31..>655
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          385..>1152
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1347
                     /gene="cnn"
                     /coded_by="XM_034334898.2:312..4355"
                     /db_xref="GeneID:117609054"
ORIGIN      
        1 mestnvkspg ktsptgvigt rdgggigrtm keyedqleal kkenfnlklr iyfleermgi
       61 tsadenaikk nielkveies lrkellekqe llsqaakafe lieeqkeass rnqaqyqqsl
      121 enerekirll ekelaeyqek vadasiyyke afgitpekal eneeklhqme elvasleaev
      181 kqvtnsleee rvwaqelese rdefrerlea etrlkenlaa ervqdieelr ekvkeleeqv
      241 lkrdtvvqqs rneliekeri ikeknaqlde kyrayeelna vsekrkkqvd qlrasiktrd
      301 daltdlnnkh ralltqfeng yakrsppssp samnpiedpl qsrmgqkltc vqgmtkkant
      361 cldwepnrer strmksplqt lsgevkdvrd likeieekda dlkrqeesrk qlvlklcnmq
      421 khvettdyrl kklegeheka iktiqgfmer qqqlenvklr keqkimelei elnrlrecen
      481 iqssrdshnq firrdfstdm tddperdpsn qqrfdemeak indlrdqiet ikaeksrlek
      541 qieveseelq grlqdkeqki evleiekqti reqlqdkeke ldklketakt egeevlered
      601 llrqlqqrdt eieeknqkie qlskelqvkt qnlqklvnte lwsknkeiak lhnhmtatyc
      661 hersrnksdm mqesasaqls tlvkelndig irvtftnevi qlnyvdgnep idvktmtdyv
      721 qklvmqknel ekevdylkwl klvsrpdiat eidgygdnqt erakkycell rthlkdlmkf
      781 mqemmktvdh adtvgnehkr ivldvlmssk ilsddfvnal eglstndlpf nleqvsarsm
      841 dnsvkkswse nllsatknhg stqsdseafs epdrtvslar iglqetqqks lnrsrfskyt
      901 ktfsdsedsl eyvpyhniyq ndlndsetsh hiqelketna llynelsalr ndltsnvsfd
      961 cefdekispl iikleksqkf ceklqssler rthelhvlkk enkqncarka qlekklvdle
     1021 smaaemnkqk aelmhykens erktaemlit lnrendalrt rikkleeene takasisalt
     1081 keldhltlsh sqilventkl tndklrleqe mrktesrcdm tvrsihdkfn keisdlnqvn
     1141 eshrvrlqel evankelrrh valceasdsa psssgvssip teailkqtce dfmqeypays
     1201 nsqywlpvsy ptmggrskts cspdlgiesd aaittmrplk dtlkitesmt nllsdedngn
     1261 snravremdn esplpiegld evealkqene tlkrrlmktr raledtfqhl sasnknkknv
     1321 ekaiakqlqi tksilkktrt yeepldn
//
LOCUS       XP_076548212            1354 aa            linear   INV 03-SEP-2025
DEFINITION  phosphodiesterase 4D interacting protein centrosomin isoform X5
            [Osmia lignaria lignaria].
ACCESSION   XP_076548212
VERSION     XP_076548212.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692097.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1354
                     /product="phosphodiesterase 4D interacting protein
                     centrosomin isoform X5"
                     /calculated_mol_wt=157177
     Region          38..>680
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          392..>1159
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     CDS             1..1354
                     /gene="cnn"
                     /coded_by="XM_076692097.1:107..4171"
                     /db_xref="GeneID:117609054"
ORIGIN      
        1 msqtlpling tnvkspgkts ptgvigtrdg ggigrtmkey edqlealkke nfnlklriyf
       61 leermgitsa denaikknie lkveieslrk ellekqells qaakafelie eqkeassrnq
      121 aqyqqslene rekirlleke laeyqekvad asiyykeafg itpekalene eklhqmeelv
      181 asleaevkqv tnsleeervw aqeleserde frerleaetr lkenlaaerv qdieelrekv
      241 keleeqvlkr dtvvqqsrne liekeriike knaqldekyr ayeelnavse krkkqvdqlr
      301 asiktrddal tdlnnkhral ltqfengyak rsppsspsam npiedplqsr mgqkltcvqg
      361 mtkkantcld wepnrerstr mksplqtlsg evkdvrdlik eieekdadlk rqeesrkqlv
      421 lklcnmqkhv ettdyrlkkl egehekaikt iqgfmerqqq lenvklrkeq kimeleieln
      481 rlreceniqs srdshnqfir rdfstdmtdd perdpsnqqr fdemeakind lrdqietika
      541 eksrlekqie veseelqgrl qdkeqkievl eiekqtireq lqdkekeldk lketaktege
      601 evleredllr qlqqrdteie eknqkieqls kelqvktqnl qklvntelws knkeiaklhn
      661 hmtatycher srnksdmmqe sasaqlstlv kelndigirv tftneviqln yvdgnepidv
      721 ktmtdyvqkl vmqkneleke vdylkwlklv srpdiateid gygdnqtera kkycellrth
      781 lkdlmkfmqe mmktvdhadt vgnehkrivl dvlmsskils ddfvnalegl stndlpfnle
      841 qvsarsmdns vkkswsenll satknhgstq sdseafsepd rtvslarigl qetqqkslnr
      901 srfskytktf sdsedsleyv pyhniyqndl ndsetshhiq elketnally nelsalrndl
      961 tsnvsfdcef dekispliik leksqkfcek lqsslerrth elhvlkkenk qncarkaqle
     1021 kklvdlesma aemnkqkael mhykenserk taemlitlnr endalrtrik kleeenetak
     1081 asisaltkel dhltlshsqi lventkltnd klrleqemrk tesrcdmtvr sihdkfnkei
     1141 sdlnqvnesh rvrlqeleva nkelrrhval ceasdsapss sgvssiptea ilkqtcedfm
     1201 qeypaysnsq ywlpvsyptm ggrsktscsp dlgiesdaai ttmrplkdtl kitesmtnll
     1261 sdedngnsnr avremdnesp lpiegldeve alkqenetlk rrlmktrral edtfqhlsas
     1321 nknkknveka iakqlqitks ilkktrtyee pldn
//
LOCUS       XP_034190738            1121 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609016 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190738
VERSION     XP_034190738.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334847.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190738.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1121
                     /product="uncharacterized protein LOC117609016 isoform X1"
                     /calculated_mol_wt=128334
     Region          208..432
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..1121
                     /gene="LOC117609016"
                     /coded_by="XM_034334847.2:65..3430"
                     /db_xref="GeneID:117609016"
ORIGIN      
        1 mivpqlefea ngersvsrvv rsvrrkcrgd tnvrqagvvs rgkeadrhdp hassrsklli
       61 gvflgiavft chlidsylra vcrcatcamg mmgmfkwlrk dviqrdrngw nlaqkqcgln
      121 nesyrnalqa fidrrrlsdd vdtdtpaspd kkradlltkn nnnkcdnerk chelkscvlg
      181 vprippseif eigwvagted rylelifaew qntrvslkrh shpeckdavk adldvlseir
      241 hpnvlllmas tftddhglvs ilesidctly hyihdqgeri siqgiarcgg rlsdalrhsh
      301 mrgyvhtain shcvylasng mvklggwela mhvnnpkper eyeerlrtei frwqapelfr
      361 syearkesdv ygltlliwem ctmrvpwsgy skadverqym hwkrgvimdl ydfppllnnl
      421 leaglqldvn krtldmnrmr rflqrlemqy edeepvyidq yannnndtgk tfvspvtrsp
      481 mgiskskslk ltksastrql yparnpespt sqylyskrsk qtrsvkatah ieqvnffged
      541 vkrttndniq knmnvlcsah nnykkhnypn cnietldvap ndevnseasq pwnstykkvk
      601 gpqetmesrs tsvyssplld stddesfkda rtnlkqlkkv lanrrehffy gsdsshtsad
      661 tslnakteil nqtkskdyep hkpashktsl epkynkspnq ydpsymkssl qqcrkipylh
      721 tpesikdait qrkvlnsdpq sffetslwrk ekliciskmr ksycdepsqy taqhtddndt
      781 vstetftlaq tneytspaks dntyvinktp eaaetennqe iltqisgnsn dsmetisprs
      841 kplqvlkdal dratkivrsv tpntdescps ppfksfqefe dtmdngqsck sifedlynlr
      901 mnrdnekiri ierdttkdes flfnknnmts nkvqqkpgne vtisinsrek sscsydasyn
      961 rsficesiae vssefnnscn diksdkefrn detsniegtv qtstmtidqs svddrrevke
     1021 nevkdfnlmf lndssknknc kscyhnalpr rrslpaalnq lrsmnnsalg klpirrgdvp
     1081 dstvedlyid defgdslnvn mvlldedltv dedfltkise l
//
LOCUS       XP_034190739            1020 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609016 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190739
VERSION     XP_034190739.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334848.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190739.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1020
                     /product="uncharacterized protein LOC117609016 isoform X2"
                     /calculated_mol_wt=117050
     Region          107..331
                     /region_name="PKc_like"
                     /note="Protein Kinases, catalytic domain; cl21453"
                     /db_xref="CDD:473864"
     CDS             1..1020
                     /gene="LOC117609016"
                     /coded_by="XM_034334848.2:104..3166"
                     /db_xref="GeneID:117609016"
ORIGIN      
        1 mtdgvkkrhe rryscgrtvs esyrnalqaf idrrrlsddv dtdtpaspdk kradlltknn
       61 nnkcdnerkc helkscvlgv prippseife igwvagtedr ylelifaewq ntrvslkrhs
      121 hpeckdavka dldvlseirh pnvlllmast ftddhglvsi lesidctlyh yihdqgeris
      181 iqgiarcggr lsdalrhshm rgyvhtains hcvylasngm vklggwelam hvnnpkpere
      241 yeerlrteif rwqapelfrs yearkesdvy gltlliwemc tmrvpwsgys kadverqymh
      301 wkrgvimdly dfppllnnll eaglqldvnk rtldmnrmrr flqrlemqye deepvyidqy
      361 annnndtgkt fvspvtrspm giskskslkl tksastrqly parnpespts qylyskrskq
      421 trsvkatahi eqvnffgedv krttndniqk nmnvlcsahn nykkhnypnc nietldvapn
      481 devnseasqp wnstykkvkg pqetmesrst svysspllds tddesfkdar tnlkqlkkvl
      541 anrrehffyg sdsshtsadt slnakteiln qtkskdyeph kpashktsle pkynkspnqy
      601 dpsymksslq qcrkipylht pesikdaitq rkvlnsdpqs ffetslwrke kliciskmrk
      661 sycdepsqyt aqhtddndtv stetftlaqt neytspaksd ntyvinktpe aaetennqei
      721 ltqisgnsnd smetisprsk plqvlkdald ratkivrsvt pntdescpsp pfksfqefed
      781 tmdngqscks ifedlynlrm nrdnekirii erdttkdesf lfnknnmtsn kvqqkpgnev
      841 tisinsreks scsydasynr sficesiaev ssefnnscnd iksdkefrnd etsniegtvq
      901 tstmtidqss vddrrevken evkdfnlmfl ndssknknck scyhnalprr rslpaalnql
      961 rsmnnsalgk lpirrgdvpd stvedlyidd efgdslnvnm vlldedltvd edfltkisel
//
LOCUS       XP_034190790             649 aa            linear   INV 03-SEP-2025
DEFINITION  long-chain fatty acid transport protein 4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190790
VERSION     XP_034190790.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334899.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="long-chain fatty acid transport protein 4"
                     /calculated_mol_wt=72752
     Region          47..649
                     /region_name="Adenylate forming domain, Class I
                     superfamily"
                     /note="This family includes acyl- and aryl-CoA ligases, as
                     well as the adenylation domain of nonribosomal peptide
                     synthetases and firefly luciferases. The adenylate-forming
                     enzymes catalyze an ATP-dependent two-step reaction to
                     first activate a carboxylate...; cl17068"
                     /db_xref="CDD:473059"
     Site            order(250,253..258,260..261)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(253,367..368,388..393,496,508,511,522,602)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(253,292..293,349,351..352,355,367..368,388..393,496,
                     508,511,519..522,583)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(292,351..352,355,367,519..521,574,583)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..649
                     /gene="LOC117609055"
                     /coded_by="XM_034334899.2:349..2298"
                     /db_xref="GeneID:117609055"
ORIGIN      
        1 mdirllllil aiigamfgav atrfgfpgfn kiaqivlava llpvlcrfhr klyvickvlp
       61 rdmkflyrav taeletkshr rnnmtvtkvf rkrvehypnk pcfffedrvw tnadinkysn
      121 qiasvfqkag yvkgdavalm mpnrpeyvai wlglgklgvv talintnlrl qslihcvria
      181 evksiiyved yssaindikd sihgidrykv cfksethedg aidlnkllse asteepvvkd
      241 epgyrdklly iytsgttglp kvaivlnsry llvvmpfnll girssdilyn pnplyhttgg
      301 migagfailk giptvlrpkf svsaywtdci kynctaaqfv gemcryllna ppkpednahr
      361 lrlmfgngmr pqiwnefvtr fnikrvaefy gssegnania nldgkagavg fvpliiprrf
      421 hplaiirvns etyepirgpn glciraetne pgmfiglike gnalrefngy ldkeaskkki
      481 iqdvfvkgdk afltgdilve decgyiyfkd rvgdtfrwkg envataeveg visniagqrd
      541 atvygvqvpg megragmaai vdpdslldfk alaeglekal payarpiflr ivkelemtgt
      601 fklkkvnlqk egfdpskvqd kmyflsgnke yveitpelyk eiisgtrkf
//
LOCUS       XP_034190791             649 aa            linear   INV 03-SEP-2025
DEFINITION  long-chain fatty acid transport protein 4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190791
VERSION     XP_034190791.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334900.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="long-chain fatty acid transport protein 4"
                     /calculated_mol_wt=72752
     Region          47..649
                     /region_name="Adenylate forming domain, Class I
                     superfamily"
                     /note="This family includes acyl- and aryl-CoA ligases, as
                     well as the adenylation domain of nonribosomal peptide
                     synthetases and firefly luciferases. The adenylate-forming
                     enzymes catalyze an ATP-dependent two-step reaction to
                     first activate a carboxylate...; cl17068"
                     /db_xref="CDD:473059"
     Site            order(250,253..258,260..261)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(253,367..368,388..393,496,508,511,522,602)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(253,292..293,349,351..352,355,367..368,388..393,496,
                     508,511,519..522,583)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(292,351..352,355,367,519..521,574,583)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..649
                     /gene="LOC117609055"
                     /coded_by="XM_034334900.2:543..2492"
                     /db_xref="GeneID:117609055"
ORIGIN      
        1 mdirllllil aiigamfgav atrfgfpgfn kiaqivlava llpvlcrfhr klyvickvlp
       61 rdmkflyrav taeletkshr rnnmtvtkvf rkrvehypnk pcfffedrvw tnadinkysn
      121 qiasvfqkag yvkgdavalm mpnrpeyvai wlglgklgvv talintnlrl qslihcvria
      181 evksiiyved yssaindikd sihgidrykv cfksethedg aidlnkllse asteepvvkd
      241 epgyrdklly iytsgttglp kvaivlnsry llvvmpfnll girssdilyn pnplyhttgg
      301 migagfailk giptvlrpkf svsaywtdci kynctaaqfv gemcryllna ppkpednahr
      361 lrlmfgngmr pqiwnefvtr fnikrvaefy gssegnania nldgkagavg fvpliiprrf
      421 hplaiirvns etyepirgpn glciraetne pgmfiglike gnalrefngy ldkeaskkki
      481 iqdvfvkgdk afltgdilve decgyiyfkd rvgdtfrwkg envataeveg visniagqrd
      541 atvygvqvpg megragmaai vdpdslldfk alaeglekal payarpiflr ivkelemtgt
      601 fklkkvnlqk egfdpskvqd kmyflsgnke yveitpelyk eiisgtrkf
//
LOCUS       XP_076548213             649 aa            linear   INV 03-SEP-2025
DEFINITION  long-chain fatty acid transport protein 4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548213
VERSION     XP_076548213.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692098.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..649
                     /product="long-chain fatty acid transport protein 4"
                     /calculated_mol_wt=72752
     Region          47..649
                     /region_name="Adenylate forming domain, Class I
                     superfamily"
                     /note="This family includes acyl- and aryl-CoA ligases, as
                     well as the adenylation domain of nonribosomal peptide
                     synthetases and firefly luciferases. The adenylate-forming
                     enzymes catalyze an ATP-dependent two-step reaction to
                     first activate a carboxylate...; cl17068"
                     /db_xref="CDD:473059"
     Site            order(250,253..258,260..261)
                     /site_type="other"
                     /note="acyl-activating enzyme (AAE) consensus motif"
                     /db_xref="CDD:341228"
     Site            order(253,367..368,388..393,496,508,511,522,602)
                     /site_type="other"
                     /note="AMP binding site [chemical binding]"
                     /db_xref="CDD:341228"
     Site            order(253,292..293,349,351..352,355,367..368,388..393,496,
                     508,511,519..522,583)
                     /site_type="active"
                     /db_xref="CDD:341228"
     Site            order(292,351..352,355,367,519..521,574,583)
                     /site_type="other"
                     /note="CoA binding site [chemical binding]"
                     /db_xref="CDD:341228"
     CDS             1..649
                     /gene="LOC117609055"
                     /coded_by="XM_076692098.1:71..2020"
                     /db_xref="GeneID:117609055"
ORIGIN      
        1 mdirllllil aiigamfgav atrfgfpgfn kiaqivlava llpvlcrfhr klyvickvlp
       61 rdmkflyrav taeletkshr rnnmtvtkvf rkrvehypnk pcfffedrvw tnadinkysn
      121 qiasvfqkag yvkgdavalm mpnrpeyvai wlglgklgvv talintnlrl qslihcvria
      181 evksiiyved yssaindikd sihgidrykv cfksethedg aidlnkllse asteepvvkd
      241 epgyrdklly iytsgttglp kvaivlnsry llvvmpfnll girssdilyn pnplyhttgg
      301 migagfailk giptvlrpkf svsaywtdci kynctaaqfv gemcryllna ppkpednahr
      361 lrlmfgngmr pqiwnefvtr fnikrvaefy gssegnania nldgkagavg fvpliiprrf
      421 hplaiirvns etyepirgpn glciraetne pgmfiglike gnalrefngy ldkeaskkki
      481 iqdvfvkgdk afltgdilve decgyiyfkd rvgdtfrwkg envataeveg visniagqrd
      541 atvygvqvpg megragmaai vdpdslldfk alaeglekal payarpiflr ivkelemtgt
      601 fklkkvnlqk egfdpskvqd kmyflsgnke yveitpelyk eiisgtrkf
//
LOCUS       XP_034190792             316 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609056 [Osmia lignaria lignaria].
ACCESSION   XP_034190792
VERSION     XP_034190792.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334901.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..316
                     /product="uncharacterized protein LOC117609056"
                     /calculated_mol_wt=36149
     Region          176..271
                     /region_name="BRICHOS"
                     /note="The BRICHOS domain is found in a variety of
                     proteins implicated in dementia, respiratory distress and
                     cancer; smart01039"
                     /db_xref="CDD:198107"
     CDS             1..316
                     /gene="LOC117609056"
                     /coded_by="XM_034334901.2:181..1131"
                     /db_xref="GeneID:117609056"
ORIGIN      
        1 mtiitkalte kktdkkeqal fveenagets krdpeaqrqk sdvgghffvs rrsihhihvt
       61 atfslflval miliigvigg lyiyrqyart qmhrfrtgwy sipydnsnka syaesaaqqs
      121 lmadldlllr ssikdeqqev msieknfdgn issffneqfe fdlenehyek idvpdfrggr
      181 qgrfihdfni nktgiidvdg rccfvmplnr ktvlpprnmy dllrkiyngy yevdteivre
      241 tmkvvtppit dlsvvgtyia recqdlptym ltkvnsnvvk rsissgvfgq fagkniiefd
      301 ilnlvdveay qkssqn
//
LOCUS       XP_034190793             303 aa            linear   INV 03-SEP-2025
DEFINITION  distal membrane arm assembly component 2 [Osmia lignaria lignaria].
ACCESSION   XP_034190793
VERSION     XP_034190793.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334902.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190793.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..303
                     /product="distal membrane arm assembly component 2"
                     /calculated_mol_wt=35972
     Region          181..>286
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          181..204
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          205..230
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          231..255
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..303
                     /gene="LOC117609057"
                     /coded_by="XM_034334902.2:1..912"
                     /db_xref="GeneID:117609057"
ORIGIN      
        1 mlhsykmlhn nfltkmfqys thvttkqife clahrnkicn ntarlfyndr nsidylvkyl
       61 rkrqdtidly vedeekvtfk kktnsgplsm myfnptapei hdfftynwir wwkhykvqlr
      121 kklqkyryer nqalgpdlas akfvivsggr vkftnqndwi dnsntteian fpkeyqpdfv
      181 legldlrgyp leyenfyvis hlhhlkclil rgcntindws idkiageypt leyldiseck
      241 nvtergleal ykmpnlkqll vtnfydsagf eltclmledv nphlkceilk lekdmllvrg
      301 yek
//
LOCUS       XP_034190795             182 aa            linear   INV 03-SEP-2025
DEFINITION  U11/U12 small nuclear ribonucleoprotein 25 kDa protein [Osmia
            lignaria lignaria].
ACCESSION   XP_034190795
VERSION     XP_034190795.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334904.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..182
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..182
                     /product="U11/U12 small nuclear ribonucleoprotein 25 kDa
                     protein"
                     /calculated_mol_wt=20880
     Region          82..171
                     /region_name="Ubl_SNRNP25"
                     /note="ubiquitin-like (Ubl) domain found in small nuclear
                     ribonucleoprotein U11/U12 subunit 25 (SNRNP25) and similar
                     proteins; cd17058"
                     /db_xref="CDD:340578"
     CDS             1..182
                     /gene="LOC117609060"
                     /coded_by="XM_034334904.2:1..549"
                     /db_xref="GeneID:117609060"
ORIGIN      
        1 mdqaqstvkd sklenvehkn kyllkneeii dkqseldhne lvkltkeaid niiesdplfs
       61 glpsdvtvee lkaqiavaqg qaitlylnrg elpklgivvp phnttvlnlk kaikrhtnls
      121 lkrenvkkki swthiwkkyh lcfdnvklin dnenikaygv tnkaelyyvk krreknklik
      181 nt
//
LOCUS       XP_034190740             112 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light chain 90F [Osmia lignaria lignaria].
ACCESSION   XP_034190740
VERSION     XP_034190740.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334849.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..112
                     /product="dynein light chain 90F"
                     /calculated_mol_wt=12401
     Region          11..112
                     /region_name="DLC-like_DYNLT1"
                     /note="dynein light chain (DLC)-like domain found in
                     dynein light chain Tctex-type 1 (DYNLT1) and similar
                     proteins; cd21462"
                     /db_xref="CDD:412010"
     Site            order(61,63..69,73..83,85,89,108,110)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:412010"
     CDS             1..112
                     /gene="Dlc90F"
                     /coded_by="XM_034334849.2:329..667"
                     /db_xref="GeneID:117609018"
ORIGIN      
        1 mmedmqeeiq fvvddvskii keaievsigg ntyqhnkvnq wasnvvegcl gnltklqkpy
       61 kyivtctimq kngaglhtas scfwdnatdg sctvrwenkt mycivsvfgl ai
//
LOCUS       XP_034190741             112 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light chain 90F [Osmia lignaria lignaria].
ACCESSION   XP_034190741
VERSION     XP_034190741.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334850.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..112
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..112
                     /product="dynein light chain 90F"
                     /calculated_mol_wt=12401
     Region          11..112
                     /region_name="DLC-like_DYNLT1"
                     /note="dynein light chain (DLC)-like domain found in
                     dynein light chain Tctex-type 1 (DYNLT1) and similar
                     proteins; cd21462"
                     /db_xref="CDD:412010"
     Site            order(61,63..69,73..83,85,89,108,110)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:412010"
     CDS             1..112
                     /gene="Dlc90F"
                     /coded_by="XM_034334850.2:222..560"
                     /db_xref="GeneID:117609018"
ORIGIN      
        1 mmedmqeeiq fvvddvskii keaievsigg ntyqhnkvnq wasnvvegcl gnltklqkpy
       61 kyivtctimq kngaglhtas scfwdnatdg sctvrwenkt mycivsvfgl ai
//
LOCUS       XP_034190796             721 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609061 [Osmia lignaria lignaria].
ACCESSION   XP_034190796
VERSION     XP_034190796.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334905.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190796.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..721
                     /product="uncharacterized protein LOC117609061"
                     /calculated_mol_wt=83318
     Region          86..>217
                     /region_name="PRK12704"
                     /note="phosphodiesterase; Provisional"
                     /db_xref="CDD:237177"
     Region          <186..605
                     /region_name="SMC_prok_B"
                     /note="chromosome segregation protein SMC, common
                     bacterial type; TIGR02168"
                     /db_xref="CDD:274008"
     Region          651..695
                     /region_name="Grip"
                     /note="golgin-97, RanBP2alpha,Imh1p and p230/golgin-245;
                     smart00755"
                     /db_xref="CDD:197860"
     CDS             1..721
                     /gene="LOC117609061"
                     /coded_by="XM_034334905.2:183..2348"
                     /db_xref="GeneID:117609061"
ORIGIN      
        1 mfatlknkir eeigsdvstv vrnagnvrgi nsrhmsqtgs tssisgsqls ldgsreenaa
       61 spsppislkk dnsfdlklnd gmqlsakdik kfenredewr krvakkeaem lkrmekkeee
      121 wkvrllerek ewkkvvekqe keksrleeel qniertkqsl elslkdaeey kkklysfqed
      181 aeqlegfqtq emakikhlll akeqevdekt hhlkaatgei dslksevsrl rryedelnnv
      241 qdemetlrhs tqreraqlsc qlaqteeevr hlkdkvfvle qrialetndq vtvderiadl
      301 mrerallerk leeahlhlsd iktswsgkis sletqvgrls rqageegler rrveeesekl
      361 kqrikqleae ievnnvvmat kdakllrmae didematelk elrasvddev eefkrqiess
      421 skeiselkqs ltgtttklta itselahvrl slegertnns slhlevarlr edlesertss
      481 atlrvcleke kgekdsallr naqvsqdiei vkqenrrqev enaelqnrie tlennlqnks
      541 keveqamatl eetkkrmlel eevernrekm eksekvlkss lldleeqlne ktktikvlqq
      601 rltdmkktlh relrvpsssl dsdaepsaai lkpsssktit arhtttredd vnfkylkhvl
      661 ikfltsreye alhltravat llhfspeeer llqetlewkm swfgtrpnlg fgqtakaipp
      721 s
//
LOCUS       XP_076548214            1245 aa            linear   INV 03-SEP-2025
DEFINITION  myosin heavy chain 95F jaguar isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548214
VERSION     XP_076548214.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692099.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="myosin heavy chain 95F jaguar isoform X1"
                     /calculated_mol_wt=144183
     Region          69..752
                     /region_name="MYSc_Myo6"
                     /note="class VI myosin, motor domain; cd01382"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105,149..156,194..204,456..461)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105)
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276833"
     Site            149..156
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276833"
     Site            194..204
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276833"
     Site            456..461
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276833"
     Site            order(485..496,499..508)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276833"
     Site            686..696
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276833"
     Site            698..752
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276833"
     Region          763..910
                     /region_name="CBD_MYO6-like"
                     /note="calmodulin binding domain found in unconventional
                     myosin-VI and similar proteins; cd21759"
                     /db_xref="CDD:409646"
     Site            order(779,782..783,785..803,805..806,809..824,826,
                     829..830,833..834,878)
                     /site_type="other"
                     /note="calmodulin binding site [polypeptide binding]"
                     /db_xref="CDD:409646"
     Site            813..823
                     /site_type="other"
                     /note="IQ motif"
                     /db_xref="CDD:409646"
     Region          902..>1016
                     /region_name="ATAD3_N"
                     /note="ATPase family AAA domain-containing protein 3,
                     N-terminal; pfam12037"
                     /db_xref="CDD:463442"
     Region          998..1075
                     /region_name="MYO6_MIU_linker"
                     /note="MIU-linker domain found in unconventional
                     myosin-VI; cl46503"
                     /db_xref="CDD:480843"
     Site            order(998..1014,1016..1021)
                     /site_type="other"
                     /note="MIU motif"
                     /db_xref="CDD:412090"
     Region          1058..1098
                     /region_name="MyUb_Myo6"
                     /note="myosin VI ubiquitin-binding domain (MyUb) found in
                     unconventional myosin-VI and similar proteins; cd21958"
                     /db_xref="CDD:439319"
     Site            order(1063..1064,1066..1067,1093..1094,1096..1097)
                     /site_type="other"
                     /note="clathrin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Site            order(1066..1068,1071..1072,1074..1078,1080,1083..1084,
                     1087,1090..1091)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Region          1141..1234
                     /region_name="Myosin-VI_CBD"
                     /note="Myosin VI cargo binding domain; pfam16521"
                     /db_xref="CDD:465157"
     CDS             1..1245
                     /gene="jar"
                     /coded_by="XM_076692099.1:163..3900"
                     /db_xref="GeneID:117607364"
ORIGIN      
        1 menqqvwvrd pqegfiigkf tdmvdgdali vpldpkypqr tcpleevypa geytkdvedn
       61 calmylneat llnnirtryf kdriytyvan iliavnpyce vrdlyspqti kayqgkslge
      121 tpphvfaiad kafrdmkvlk qsqsiivsge sgagktestk yllrylcdlw gstagpieqk
      181 ildanpvlea fgnaktkrnn nssrfgkfme vhfdskcqvv ggyishylle ksrvclqspd
      241 ernyhvfymm cagappelra klgitkpddf hylkngctqy fcneesekkl ndvqksrdqv
      301 tkgslhdpil ddvqgfsaid qaltrlglte germeiytmv aavlhlgnim fednpedtkg
      361 gsricadsek avlmsaklla vdpeelrqal vskvmqtsrg gikgtvimvp lkvyeannar
      421 dalakaiysk lfdhivarin ksipfkassy yigvldiagf eyfkvnsfeq fcinycnekl
      481 qqffnerilk yeqdlyares lnvpkisyvd nqdcidlies knmgifslld eesklpthsf
      541 ahftsevhrv wnghfritlp rtsrlkahre lrddegfvir hyaggvcyqt nqfieknnda
      601 lhaslealiq esnnqflrtq fannscqkgk ltfisvgskf ktqlgelmnk lksngtnfir
      661 cikpnndmvd hqfdgnsilg qlrcsgmtsv lelmehgyps rvpfqelynm yksylppela
      721 kleprffcea llhslklnkk dfkfgitrvf fkpgkfaefd kimksdpenl kklvanvkkw
      781 llrsrwnkmq fcalsviklk nkiiyrrqhl iilqktarmy iakkqhrpri kgimeiknlk
      841 tqlvgmeets kqlknrekhm sdikklqdnl daainkikns mkiketeidn lytnlvnqvn
      901 kemaslqdmv rqqkiaeeqa klrkiqqele lekqrkdeee rqkreeeenr kkkheietrr
      961 kaeeeqkrrq eeedkktaaa lqaqleream eenryrelle qerrdhelav rlaqesngqv
     1021 edsplpvrsg sdvrvpsnnn rlarseqvrn nqaamsnkky dlskwkysel rdaintscdi
     1081 elleacrhef hrrlkvyhaw karnrrritm denerapksi meaaaktprs qmkqtiness
     1141 qryfripfvr ppapgqyens hitakrgwwy ahfdgqyvar qmelhpdkap illvagiddm
     1201 qmcelsldet gltrkrgaei lehefnrewe khggkpyips cdrkk
//
LOCUS       XP_034186869            1245 aa            linear   INV 03-SEP-2025
DEFINITION  myosin heavy chain 95F jaguar isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186869
VERSION     XP_034186869.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330978.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="myosin heavy chain 95F jaguar isoform X1"
                     /calculated_mol_wt=144183
     Region          69..752
                     /region_name="MYSc_Myo6"
                     /note="class VI myosin, motor domain; cd01382"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105,149..156,194..204,456..461)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105)
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276833"
     Site            149..156
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276833"
     Site            194..204
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276833"
     Site            456..461
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276833"
     Site            order(485..496,499..508)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276833"
     Site            686..696
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276833"
     Site            698..752
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276833"
     Region          763..910
                     /region_name="CBD_MYO6-like"
                     /note="calmodulin binding domain found in unconventional
                     myosin-VI and similar proteins; cd21759"
                     /db_xref="CDD:409646"
     Site            order(779,782..783,785..803,805..806,809..824,826,
                     829..830,833..834,878)
                     /site_type="other"
                     /note="calmodulin binding site [polypeptide binding]"
                     /db_xref="CDD:409646"
     Site            813..823
                     /site_type="other"
                     /note="IQ motif"
                     /db_xref="CDD:409646"
     Region          902..>1016
                     /region_name="ATAD3_N"
                     /note="ATPase family AAA domain-containing protein 3,
                     N-terminal; pfam12037"
                     /db_xref="CDD:463442"
     Region          998..1075
                     /region_name="MYO6_MIU_linker"
                     /note="MIU-linker domain found in unconventional
                     myosin-VI; cl46503"
                     /db_xref="CDD:480843"
     Site            order(998..1014,1016..1021)
                     /site_type="other"
                     /note="MIU motif"
                     /db_xref="CDD:412090"
     Region          1058..1098
                     /region_name="MyUb_Myo6"
                     /note="myosin VI ubiquitin-binding domain (MyUb) found in
                     unconventional myosin-VI and similar proteins; cd21958"
                     /db_xref="CDD:439319"
     Site            order(1063..1064,1066..1067,1093..1094,1096..1097)
                     /site_type="other"
                     /note="clathrin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Site            order(1066..1068,1071..1072,1074..1078,1080,1083..1084,
                     1087,1090..1091)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Region          1141..1234
                     /region_name="Myosin-VI_CBD"
                     /note="Myosin VI cargo binding domain; pfam16521"
                     /db_xref="CDD:465157"
     CDS             1..1245
                     /gene="jar"
                     /coded_by="XM_034330978.2:530..4267"
                     /db_xref="GeneID:117607364"
ORIGIN      
        1 menqqvwvrd pqegfiigkf tdmvdgdali vpldpkypqr tcpleevypa geytkdvedn
       61 calmylneat llnnirtryf kdriytyvan iliavnpyce vrdlyspqti kayqgkslge
      121 tpphvfaiad kafrdmkvlk qsqsiivsge sgagktestk yllrylcdlw gstagpieqk
      181 ildanpvlea fgnaktkrnn nssrfgkfme vhfdskcqvv ggyishylle ksrvclqspd
      241 ernyhvfymm cagappelra klgitkpddf hylkngctqy fcneesekkl ndvqksrdqv
      301 tkgslhdpil ddvqgfsaid qaltrlglte germeiytmv aavlhlgnim fednpedtkg
      361 gsricadsek avlmsaklla vdpeelrqal vskvmqtsrg gikgtvimvp lkvyeannar
      421 dalakaiysk lfdhivarin ksipfkassy yigvldiagf eyfkvnsfeq fcinycnekl
      481 qqffnerilk yeqdlyares lnvpkisyvd nqdcidlies knmgifslld eesklpthsf
      541 ahftsevhrv wnghfritlp rtsrlkahre lrddegfvir hyaggvcyqt nqfieknnda
      601 lhaslealiq esnnqflrtq fannscqkgk ltfisvgskf ktqlgelmnk lksngtnfir
      661 cikpnndmvd hqfdgnsilg qlrcsgmtsv lelmehgyps rvpfqelynm yksylppela
      721 kleprffcea llhslklnkk dfkfgitrvf fkpgkfaefd kimksdpenl kklvanvkkw
      781 llrsrwnkmq fcalsviklk nkiiyrrqhl iilqktarmy iakkqhrpri kgimeiknlk
      841 tqlvgmeets kqlknrekhm sdikklqdnl daainkikns mkiketeidn lytnlvnqvn
      901 kemaslqdmv rqqkiaeeqa klrkiqqele lekqrkdeee rqkreeeenr kkkheietrr
      961 kaeeeqkrrq eeedkktaaa lqaqleream eenryrelle qerrdhelav rlaqesngqv
     1021 edsplpvrsg sdvrvpsnnn rlarseqvrn nqaamsnkky dlskwkysel rdaintscdi
     1081 elleacrhef hrrlkvyhaw karnrrritm denerapksi meaaaktprs qmkqtiness
     1141 qryfripfvr ppapgqyens hitakrgwwy ahfdgqyvar qmelhpdkap illvagiddm
     1201 qmcelsldet gltrkrgaei lehefnrewe khggkpyips cdrkk
//
LOCUS       XP_034186870            1245 aa            linear   INV 03-SEP-2025
DEFINITION  myosin heavy chain 95F jaguar isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186870
VERSION     XP_034186870.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330979.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="myosin heavy chain 95F jaguar isoform X1"
                     /calculated_mol_wt=144183
     Region          69..752
                     /region_name="MYSc_Myo6"
                     /note="class VI myosin, motor domain; cd01382"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105,149..156,194..204,456..461)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105)
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276833"
     Site            149..156
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276833"
     Site            194..204
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276833"
     Site            456..461
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276833"
     Site            order(485..496,499..508)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276833"
     Site            686..696
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276833"
     Site            698..752
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276833"
     Region          763..910
                     /region_name="CBD_MYO6-like"
                     /note="calmodulin binding domain found in unconventional
                     myosin-VI and similar proteins; cd21759"
                     /db_xref="CDD:409646"
     Site            order(779,782..783,785..803,805..806,809..824,826,
                     829..830,833..834,878)
                     /site_type="other"
                     /note="calmodulin binding site [polypeptide binding]"
                     /db_xref="CDD:409646"
     Site            813..823
                     /site_type="other"
                     /note="IQ motif"
                     /db_xref="CDD:409646"
     Region          902..>1016
                     /region_name="ATAD3_N"
                     /note="ATPase family AAA domain-containing protein 3,
                     N-terminal; pfam12037"
                     /db_xref="CDD:463442"
     Region          998..1075
                     /region_name="MYO6_MIU_linker"
                     /note="MIU-linker domain found in unconventional
                     myosin-VI; cl46503"
                     /db_xref="CDD:480843"
     Site            order(998..1014,1016..1021)
                     /site_type="other"
                     /note="MIU motif"
                     /db_xref="CDD:412090"
     Region          1058..1098
                     /region_name="MyUb_Myo6"
                     /note="myosin VI ubiquitin-binding domain (MyUb) found in
                     unconventional myosin-VI and similar proteins; cd21958"
                     /db_xref="CDD:439319"
     Site            order(1063..1064,1066..1067,1093..1094,1096..1097)
                     /site_type="other"
                     /note="clathrin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Site            order(1066..1068,1071..1072,1074..1078,1080,1083..1084,
                     1087,1090..1091)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Region          1141..1234
                     /region_name="Myosin-VI_CBD"
                     /note="Myosin VI cargo binding domain; pfam16521"
                     /db_xref="CDD:465157"
     CDS             1..1245
                     /gene="jar"
                     /coded_by="XM_034330979.2:107..3844"
                     /db_xref="GeneID:117607364"
ORIGIN      
        1 menqqvwvrd pqegfiigkf tdmvdgdali vpldpkypqr tcpleevypa geytkdvedn
       61 calmylneat llnnirtryf kdriytyvan iliavnpyce vrdlyspqti kayqgkslge
      121 tpphvfaiad kafrdmkvlk qsqsiivsge sgagktestk yllrylcdlw gstagpieqk
      181 ildanpvlea fgnaktkrnn nssrfgkfme vhfdskcqvv ggyishylle ksrvclqspd
      241 ernyhvfymm cagappelra klgitkpddf hylkngctqy fcneesekkl ndvqksrdqv
      301 tkgslhdpil ddvqgfsaid qaltrlglte germeiytmv aavlhlgnim fednpedtkg
      361 gsricadsek avlmsaklla vdpeelrqal vskvmqtsrg gikgtvimvp lkvyeannar
      421 dalakaiysk lfdhivarin ksipfkassy yigvldiagf eyfkvnsfeq fcinycnekl
      481 qqffnerilk yeqdlyares lnvpkisyvd nqdcidlies knmgifslld eesklpthsf
      541 ahftsevhrv wnghfritlp rtsrlkahre lrddegfvir hyaggvcyqt nqfieknnda
      601 lhaslealiq esnnqflrtq fannscqkgk ltfisvgskf ktqlgelmnk lksngtnfir
      661 cikpnndmvd hqfdgnsilg qlrcsgmtsv lelmehgyps rvpfqelynm yksylppela
      721 kleprffcea llhslklnkk dfkfgitrvf fkpgkfaefd kimksdpenl kklvanvkkw
      781 llrsrwnkmq fcalsviklk nkiiyrrqhl iilqktarmy iakkqhrpri kgimeiknlk
      841 tqlvgmeets kqlknrekhm sdikklqdnl daainkikns mkiketeidn lytnlvnqvn
      901 kemaslqdmv rqqkiaeeqa klrkiqqele lekqrkdeee rqkreeeenr kkkheietrr
      961 kaeeeqkrrq eeedkktaaa lqaqleream eenryrelle qerrdhelav rlaqesngqv
     1021 edsplpvrsg sdvrvpsnnn rlarseqvrn nqaamsnkky dlskwkysel rdaintscdi
     1081 elleacrhef hrrlkvyhaw karnrrritm denerapksi meaaaktprs qmkqtiness
     1141 qryfripfvr ppapgqyens hitakrgwwy ahfdgqyvar qmelhpdkap illvagiddm
     1201 qmcelsldet gltrkrgaei lehefnrewe khggkpyips cdrkk
//
LOCUS       XP_034186871            1245 aa            linear   INV 03-SEP-2025
DEFINITION  myosin heavy chain 95F jaguar isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186871
VERSION     XP_034186871.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330980.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="myosin heavy chain 95F jaguar isoform X1"
                     /calculated_mol_wt=144183
     Region          69..752
                     /region_name="MYSc_Myo6"
                     /note="class VI myosin, motor domain; cd01382"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105,149..156,194..204,456..461)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105)
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276833"
     Site            149..156
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276833"
     Site            194..204
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276833"
     Site            456..461
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276833"
     Site            order(485..496,499..508)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276833"
     Site            686..696
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276833"
     Site            698..752
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276833"
     Region          763..910
                     /region_name="CBD_MYO6-like"
                     /note="calmodulin binding domain found in unconventional
                     myosin-VI and similar proteins; cd21759"
                     /db_xref="CDD:409646"
     Site            order(779,782..783,785..803,805..806,809..824,826,
                     829..830,833..834,878)
                     /site_type="other"
                     /note="calmodulin binding site [polypeptide binding]"
                     /db_xref="CDD:409646"
     Site            813..823
                     /site_type="other"
                     /note="IQ motif"
                     /db_xref="CDD:409646"
     Region          902..>1016
                     /region_name="ATAD3_N"
                     /note="ATPase family AAA domain-containing protein 3,
                     N-terminal; pfam12037"
                     /db_xref="CDD:463442"
     Region          998..1075
                     /region_name="MYO6_MIU_linker"
                     /note="MIU-linker domain found in unconventional
                     myosin-VI; cl46503"
                     /db_xref="CDD:480843"
     Site            order(998..1014,1016..1021)
                     /site_type="other"
                     /note="MIU motif"
                     /db_xref="CDD:412090"
     Region          1058..1098
                     /region_name="MyUb_Myo6"
                     /note="myosin VI ubiquitin-binding domain (MyUb) found in
                     unconventional myosin-VI and similar proteins; cd21958"
                     /db_xref="CDD:439319"
     Site            order(1063..1064,1066..1067,1093..1094,1096..1097)
                     /site_type="other"
                     /note="clathrin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Site            order(1066..1068,1071..1072,1074..1078,1080,1083..1084,
                     1087,1090..1091)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Region          1141..1234
                     /region_name="Myosin-VI_CBD"
                     /note="Myosin VI cargo binding domain; pfam16521"
                     /db_xref="CDD:465157"
     CDS             1..1245
                     /gene="jar"
                     /coded_by="XM_034330980.2:163..3900"
                     /db_xref="GeneID:117607364"
ORIGIN      
        1 menqqvwvrd pqegfiigkf tdmvdgdali vpldpkypqr tcpleevypa geytkdvedn
       61 calmylneat llnnirtryf kdriytyvan iliavnpyce vrdlyspqti kayqgkslge
      121 tpphvfaiad kafrdmkvlk qsqsiivsge sgagktestk yllrylcdlw gstagpieqk
      181 ildanpvlea fgnaktkrnn nssrfgkfme vhfdskcqvv ggyishylle ksrvclqspd
      241 ernyhvfymm cagappelra klgitkpddf hylkngctqy fcneesekkl ndvqksrdqv
      301 tkgslhdpil ddvqgfsaid qaltrlglte germeiytmv aavlhlgnim fednpedtkg
      361 gsricadsek avlmsaklla vdpeelrqal vskvmqtsrg gikgtvimvp lkvyeannar
      421 dalakaiysk lfdhivarin ksipfkassy yigvldiagf eyfkvnsfeq fcinycnekl
      481 qqffnerilk yeqdlyares lnvpkisyvd nqdcidlies knmgifslld eesklpthsf
      541 ahftsevhrv wnghfritlp rtsrlkahre lrddegfvir hyaggvcyqt nqfieknnda
      601 lhaslealiq esnnqflrtq fannscqkgk ltfisvgskf ktqlgelmnk lksngtnfir
      661 cikpnndmvd hqfdgnsilg qlrcsgmtsv lelmehgyps rvpfqelynm yksylppela
      721 kleprffcea llhslklnkk dfkfgitrvf fkpgkfaefd kimksdpenl kklvanvkkw
      781 llrsrwnkmq fcalsviklk nkiiyrrqhl iilqktarmy iakkqhrpri kgimeiknlk
      841 tqlvgmeets kqlknrekhm sdikklqdnl daainkikns mkiketeidn lytnlvnqvn
      901 kemaslqdmv rqqkiaeeqa klrkiqqele lekqrkdeee rqkreeeenr kkkheietrr
      961 kaeeeqkrrq eeedkktaaa lqaqleream eenryrelle qerrdhelav rlaqesngqv
     1021 edsplpvrsg sdvrvpsnnn rlarseqvrn nqaamsnkky dlskwkysel rdaintscdi
     1081 elleacrhef hrrlkvyhaw karnrrritm denerapksi meaaaktprs qmkqtiness
     1141 qryfripfvr ppapgqyens hitakrgwwy ahfdgqyvar qmelhpdkap illvagiddm
     1201 qmcelsldet gltrkrgaei lehefnrewe khggkpyips cdrkk
//
LOCUS       XP_034186872            1245 aa            linear   INV 03-SEP-2025
DEFINITION  myosin heavy chain 95F jaguar isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186872
VERSION     XP_034186872.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330981.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1245
                     /product="myosin heavy chain 95F jaguar isoform X1"
                     /calculated_mol_wt=144183
     Region          69..752
                     /region_name="MYSc_Myo6"
                     /note="class VI myosin, motor domain; cd01382"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105,149..156,194..204,456..461)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105)
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276833"
     Site            149..156
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276833"
     Site            194..204
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276833"
     Site            456..461
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276833"
     Site            order(485..496,499..508)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276833"
     Site            686..696
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276833"
     Site            698..752
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276833"
     Region          763..910
                     /region_name="CBD_MYO6-like"
                     /note="calmodulin binding domain found in unconventional
                     myosin-VI and similar proteins; cd21759"
                     /db_xref="CDD:409646"
     Site            order(779,782..783,785..803,805..806,809..824,826,
                     829..830,833..834,878)
                     /site_type="other"
                     /note="calmodulin binding site [polypeptide binding]"
                     /db_xref="CDD:409646"
     Site            813..823
                     /site_type="other"
                     /note="IQ motif"
                     /db_xref="CDD:409646"
     Region          902..>1016
                     /region_name="ATAD3_N"
                     /note="ATPase family AAA domain-containing protein 3,
                     N-terminal; pfam12037"
                     /db_xref="CDD:463442"
     Region          998..1075
                     /region_name="MYO6_MIU_linker"
                     /note="MIU-linker domain found in unconventional
                     myosin-VI; cl46503"
                     /db_xref="CDD:480843"
     Site            order(998..1014,1016..1021)
                     /site_type="other"
                     /note="MIU motif"
                     /db_xref="CDD:412090"
     Region          1058..1098
                     /region_name="MyUb_Myo6"
                     /note="myosin VI ubiquitin-binding domain (MyUb) found in
                     unconventional myosin-VI and similar proteins; cd21958"
                     /db_xref="CDD:439319"
     Site            order(1063..1064,1066..1067,1093..1094,1096..1097)
                     /site_type="other"
                     /note="clathrin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Site            order(1066..1068,1071..1072,1074..1078,1080,1083..1084,
                     1087,1090..1091)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Region          1141..1234
                     /region_name="Myosin-VI_CBD"
                     /note="Myosin VI cargo binding domain; pfam16521"
                     /db_xref="CDD:465157"
     CDS             1..1245
                     /gene="jar"
                     /coded_by="XM_034330981.2:163..3900"
                     /db_xref="GeneID:117607364"
ORIGIN      
        1 menqqvwvrd pqegfiigkf tdmvdgdali vpldpkypqr tcpleevypa geytkdvedn
       61 calmylneat llnnirtryf kdriytyvan iliavnpyce vrdlyspqti kayqgkslge
      121 tpphvfaiad kafrdmkvlk qsqsiivsge sgagktestk yllrylcdlw gstagpieqk
      181 ildanpvlea fgnaktkrnn nssrfgkfme vhfdskcqvv ggyishylle ksrvclqspd
      241 ernyhvfymm cagappelra klgitkpddf hylkngctqy fcneesekkl ndvqksrdqv
      301 tkgslhdpil ddvqgfsaid qaltrlglte germeiytmv aavlhlgnim fednpedtkg
      361 gsricadsek avlmsaklla vdpeelrqal vskvmqtsrg gikgtvimvp lkvyeannar
      421 dalakaiysk lfdhivarin ksipfkassy yigvldiagf eyfkvnsfeq fcinycnekl
      481 qqffnerilk yeqdlyares lnvpkisyvd nqdcidlies knmgifslld eesklpthsf
      541 ahftsevhrv wnghfritlp rtsrlkahre lrddegfvir hyaggvcyqt nqfieknnda
      601 lhaslealiq esnnqflrtq fannscqkgk ltfisvgskf ktqlgelmnk lksngtnfir
      661 cikpnndmvd hqfdgnsilg qlrcsgmtsv lelmehgyps rvpfqelynm yksylppela
      721 kleprffcea llhslklnkk dfkfgitrvf fkpgkfaefd kimksdpenl kklvanvkkw
      781 llrsrwnkmq fcalsviklk nkiiyrrqhl iilqktarmy iakkqhrpri kgimeiknlk
      841 tqlvgmeets kqlknrekhm sdikklqdnl daainkikns mkiketeidn lytnlvnqvn
      901 kemaslqdmv rqqkiaeeqa klrkiqqele lekqrkdeee rqkreeeenr kkkheietrr
      961 kaeeeqkrrq eeedkktaaa lqaqleream eenryrelle qerrdhelav rlaqesngqv
     1021 edsplpvrsg sdvrvpsnnn rlarseqvrn nqaamsnkky dlskwkysel rdaintscdi
     1081 elleacrhef hrrlkvyhaw karnrrritm denerapksi meaaaktprs qmkqtiness
     1141 qryfripfvr ppapgqyens hitakrgwwy ahfdgqyvar qmelhpdkap illvagiddm
     1201 qmcelsldet gltrkrgaei lehefnrewe khggkpyips cdrkk
//
LOCUS       XP_034186873            1243 aa            linear   INV 03-SEP-2025
DEFINITION  myosin heavy chain 95F jaguar isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186873
VERSION     XP_034186873.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330982.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1243
                     /product="myosin heavy chain 95F jaguar isoform X2"
                     /calculated_mol_wt=143983
     Region          69..752
                     /region_name="MYSc_Myo6"
                     /note="class VI myosin, motor domain; cd01382"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105,149..156,194..204,456..461)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105)
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276833"
     Site            149..156
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276833"
     Site            194..204
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276833"
     Site            456..461
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276833"
     Site            order(485..496,499..508)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276833"
     Site            686..696
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276833"
     Site            698..752
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276833"
     Region          763..910
                     /region_name="CBD_MYO6-like"
                     /note="calmodulin binding domain found in unconventional
                     myosin-VI and similar proteins; cd21759"
                     /db_xref="CDD:409646"
     Site            order(779,782..783,785..803,805..806,809..824,826,
                     829..830,833..834,878)
                     /site_type="other"
                     /note="calmodulin binding site [polypeptide binding]"
                     /db_xref="CDD:409646"
     Site            813..823
                     /site_type="other"
                     /note="IQ motif"
                     /db_xref="CDD:409646"
     Region          996..1073
                     /region_name="MYO6_MIU_linker"
                     /note="MIU-linker domain found in unconventional
                     myosin-VI; cl46503"
                     /db_xref="CDD:480843"
     Site            order(996..1012,1014..1019)
                     /site_type="other"
                     /note="MIU motif"
                     /db_xref="CDD:412090"
     Region          1056..1096
                     /region_name="MyUb_Myo6"
                     /note="myosin VI ubiquitin-binding domain (MyUb) found in
                     unconventional myosin-VI and similar proteins; cd21958"
                     /db_xref="CDD:439319"
     Site            order(1061..1062,1064..1065,1091..1092,1094..1095)
                     /site_type="other"
                     /note="clathrin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Site            order(1064..1066,1069..1070,1072..1076,1078,1081..1082,
                     1085,1088..1089)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Region          1139..1232
                     /region_name="Myosin-VI_CBD"
                     /note="Myosin VI cargo binding domain; pfam16521"
                     /db_xref="CDD:465157"
     CDS             1..1243
                     /gene="jar"
                     /coded_by="XM_034330982.2:163..3894"
                     /db_xref="GeneID:117607364"
ORIGIN      
        1 menqqvwvrd pqegfiigkf tdmvdgdali vpldpkypqr tcpleevypa geytkdvedn
       61 calmylneat llnnirtryf kdriytyvan iliavnpyce vrdlyspqti kayqgkslge
      121 tpphvfaiad kafrdmkvlk qsqsiivsge sgagktestk yllrylcdlw gstagpieqk
      181 ildanpvlea fgnaktkrnn nssrfgkfme vhfdskcqvv ggyishylle ksrvclqspd
      241 ernyhvfymm cagappelra klgitkpddf hylkngctqy fcneesekkl ndvqksrdqv
      301 tkgslhdpil ddvqgfsaid qaltrlglte germeiytmv aavlhlgnim fednpedtkg
      361 gsricadsek avlmsaklla vdpeelrqal vskvmqtsrg gikgtvimvp lkvyeannar
      421 dalakaiysk lfdhivarin ksipfkassy yigvldiagf eyfkvnsfeq fcinycnekl
      481 qqffnerilk yeqdlyares lnvpkisyvd nqdcidlies knmgifslld eesklpthsf
      541 ahftsevhrv wnghfritlp rtsrlkahre lrddegfvir hyaggvcyqt nqfieknnda
      601 lhaslealiq esnnqflrtq fannscqkgk ltfisvgskf ktqlgelmnk lksngtnfir
      661 cikpnndmvd hqfdgnsilg qlrcsgmtsv lelmehgyps rvpfqelynm yksylppela
      721 kleprffcea llhslklnkk dfkfgitrvf fkpgkfaefd kimksdpenl kklvanvkkw
      781 llrsrwnkmq fcalsviklk nkiiyrrqhl iilqktarmy iakkqhrpri kgimeiknlk
      841 tqlvgmeets kqlknrekhm sdikklqdnl daainkikns mkiketeidn lytnlvnqvn
      901 kemaslqdmv rqqkiaeeqa klrkiqqele lekqrkdeee rqkreeeenr kkkheietrr
      961 kaeeeqkrrq eeedkktaaa lqlereamee nryrelleqe rrdhelavrl aqesngqved
     1021 splpvrsgsd vrvpsnnnrl arseqvrnnq aamsnkkydl skwkyselrd aintscdiel
     1081 leacrhefhr rlkvyhawka rnrrritmde nerapksime aaaktprsqm kqtinessqr
     1141 yfripfvrpp apgqyenshi takrgwwyah fdgqyvarqm elhpdkapil lvagiddmqm
     1201 celsldetgl trkrgaeile hefnrewekh ggkpyipscd rkk
//
LOCUS       XP_034186874            1230 aa            linear   INV 03-SEP-2025
DEFINITION  myosin heavy chain 95F jaguar isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186874
VERSION     XP_034186874.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330983.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1230
                     /product="myosin heavy chain 95F jaguar isoform X3"
                     /calculated_mol_wt=142615
     Region          69..752
                     /region_name="MYSc_Myo6"
                     /note="class VI myosin, motor domain; cd01382"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105,149..156,194..204,456..461)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:276833"
     Site            order(96..98,100..105)
                     /site_type="other"
                     /note="purine-binding loop"
                     /db_xref="CDD:276833"
     Site            149..156
                     /site_type="other"
                     /note="P-loop"
                     /db_xref="CDD:276833"
     Site            194..204
                     /site_type="other"
                     /note="switch I region"
                     /db_xref="CDD:276833"
     Site            456..461
                     /site_type="other"
                     /note="switch II region"
                     /db_xref="CDD:276833"
     Site            order(485..496,499..508)
                     /site_type="active"
                     /note="relay loop [active]"
                     /db_xref="CDD:276833"
     Site            686..696
                     /site_type="other"
                     /note="SH1 helix"
                     /db_xref="CDD:276833"
     Site            698..752
                     /site_type="other"
                     /note="converter subdomain"
                     /db_xref="CDD:276833"
     Region          763..910
                     /region_name="CBD_MYO6-like"
                     /note="calmodulin binding domain found in unconventional
                     myosin-VI and similar proteins; cd21759"
                     /db_xref="CDD:409646"
     Site            order(779,782..783,785..803,805..806,809..824,826,
                     829..830,833..834,878)
                     /site_type="other"
                     /note="calmodulin binding site [polypeptide binding]"
                     /db_xref="CDD:409646"
     Site            813..823
                     /site_type="other"
                     /note="IQ motif"
                     /db_xref="CDD:409646"
     Region          <900..>1100
                     /region_name="DUF5401"
                     /note="Family of unknown function (DUF5401); pfam17380"
                     /db_xref="CDD:375164"
     Region          998..1060
                     /region_name="MYO6_MIU_linker"
                     /note="MIU-linker domain found in unconventional
                     myosin-VI; cl46503"
                     /db_xref="CDD:480843"
     Site            order(998..1014,1015..1019)
                     /site_type="other"
                     /note="MIU motif"
                     /db_xref="CDD:412090"
     Region          1043..1083
                     /region_name="MyUb_Myo6"
                     /note="myosin VI ubiquitin-binding domain (MyUb) found in
                     unconventional myosin-VI and similar proteins; cd21958"
                     /db_xref="CDD:439319"
     Site            order(1048..1049,1051..1052,1078..1079,1081..1082)
                     /site_type="other"
                     /note="clathrin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Site            order(1051..1053,1056..1057,1059..1063,1065,1068..1069,
                     1072,1075..1076)
                     /site_type="other"
                     /note="ubiquitin binding site [polypeptide binding]"
                     /db_xref="CDD:439319"
     Region          1126..1219
                     /region_name="Myosin-VI_CBD"
                     /note="Myosin VI cargo binding domain; pfam16521"
                     /db_xref="CDD:465157"
     CDS             1..1230
                     /gene="jar"
                     /coded_by="XM_034330983.2:163..3855"
                     /db_xref="GeneID:117607364"
ORIGIN      
        1 menqqvwvrd pqegfiigkf tdmvdgdali vpldpkypqr tcpleevypa geytkdvedn
       61 calmylneat llnnirtryf kdriytyvan iliavnpyce vrdlyspqti kayqgkslge
      121 tpphvfaiad kafrdmkvlk qsqsiivsge sgagktestk yllrylcdlw gstagpieqk
      181 ildanpvlea fgnaktkrnn nssrfgkfme vhfdskcqvv ggyishylle ksrvclqspd
      241 ernyhvfymm cagappelra klgitkpddf hylkngctqy fcneesekkl ndvqksrdqv
      301 tkgslhdpil ddvqgfsaid qaltrlglte germeiytmv aavlhlgnim fednpedtkg
      361 gsricadsek avlmsaklla vdpeelrqal vskvmqtsrg gikgtvimvp lkvyeannar
      421 dalakaiysk lfdhivarin ksipfkassy yigvldiagf eyfkvnsfeq fcinycnekl
      481 qqffnerilk yeqdlyares lnvpkisyvd nqdcidlies knmgifslld eesklpthsf
      541 ahftsevhrv wnghfritlp rtsrlkahre lrddegfvir hyaggvcyqt nqfieknnda
      601 lhaslealiq esnnqflrtq fannscqkgk ltfisvgskf ktqlgelmnk lksngtnfir
      661 cikpnndmvd hqfdgnsilg qlrcsgmtsv lelmehgyps rvpfqelynm yksylppela
      721 kleprffcea llhslklnkk dfkfgitrvf fkpgkfaefd kimksdpenl kklvanvkkw
      781 llrsrwnkmq fcalsviklk nkiiyrrqhl iilqktarmy iakkqhrpri kgimeiknlk
      841 tqlvgmeets kqlknrekhm sdikklqdnl daainkikns mkiketeidn lytnlvnqvn
      901 kemaslqdmv rqqkiaeeqa klrkiqqele lekqrkdeee rqkreeeenr kkkheietrr
      961 kaeeeqkrrq eeedkktaaa lqaqleream eenryrelle qerrdhelav rlaqesngqv
     1021 edsplpvrrs eqvrnnqaam snkkydlskw kyselrdain tscdiellea crhefhrrlk
     1081 vyhawkarnr rritmdener apksimeaaa ktprsqmkqt inessqryfr ipfvrppapg
     1141 qyenshitak rgwwyahfdg qyvarqmelh pdkapillva giddmqmcel sldetgltrk
     1201 rgaeilehef nrewekhggk pyipscdrkk
//
LOCUS       XP_034186875            1050 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607366 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186875
VERSION     XP_034186875.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330984.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186875.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1050
                     /product="uncharacterized protein LOC117607366 isoform X1"
                     /calculated_mol_wt=117036
     Region          147..>289
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Region          <538..1040
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <888..1039
                     /region_name="Peptidase_C19R"
                     /note="A subfamily of peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02674"
                     /db_xref="CDD:239139"
     CDS             1..1050
                     /gene="LOC117607366"
                     /coded_by="XM_034330984.2:408..3560"
                     /db_xref="GeneID:117607366"
ORIGIN      
        1 mdnkvdvsev edismigsse ifylpevaei sevldstgls pltssldhtl tlqedkllss
       61 evnmgiedtw teansstsdy aiplppppgl ndftdvgadp isdsgtgieh gpflppgtpa
      121 lnnlfseggd sqddspmedv vlragvcglr nlgntcfmaa glqcltatpp vlrhfldlqq
      181 kgeklpppgs lmahfsvllg kmwsgrysvl rptefkqtlg ayhsqfkdyr qhdcqeflal
      241 lldslheqmn takytkncqt psstttansi nsiensngkt vssvtansns ncntdllemy
      301 dclpspecpn spnvtmagsp rdlgspigei dsimnspres plndgdedee tldsdeviee
      361 ktntfihnkv dekesevsps lrldtliels knvpkyhgly dihkeaktsn anflvtqqec
      421 nneihydsqk fpkdnvrrmp ldnsnlmenh dfdnksisvk rikevniqkv ncgvdyvssg
      481 sdieydngle kcnvkrmrld dqeknhkrdg qgsissqctr isqsfgngai aaqdeieadk
      541 hwakhlrsnr siivdtfqgq fkskvvcavc khvsvtyepf mylsvplpha merqlnvtyv
      601 pangdqpirc vvslnkqsri gklkeellki lgkeniaian ialaevlenh iskilddntl
      661 lrhindtnrs iyafeltepp daytsvpdgg gdrvmdtdnc qkcavtgeei qcmicleeld
      721 gglkrhsgns cnfvicdtci enyfknqtep qmcsvcstym tassfvkidq tgrpairiln
      781 vplvlrqdtt nettnnrkgt klfgyphlvk lpsrvnakdl ydivkrnvpq egnytlhfvt
      841 gqghhcsrci ytahctgcsv pesgvvilht gdtlavryte hvpkiappid hisvskqrph
      901 hplslydclq afsqsetlde hnpwfcpkce rnqcatktlt vhrypkfliv ylkrfvfyec
      961 asmklddkvt fplvglsvgq hlydlyacvc hfggvsaghy tayaknprtd vwyyyndeit
     1021 srqkpqdedf snayilfysr qgtnlkpcni
//
LOCUS       XP_034186877            1050 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607366 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186877
VERSION     XP_034186877.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330986.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186877.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1050
                     /product="uncharacterized protein LOC117607366 isoform X1"
                     /calculated_mol_wt=117036
     Region          147..>289
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Region          <538..1040
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     Region          <888..1039
                     /region_name="Peptidase_C19R"
                     /note="A subfamily of peptidase C19. Peptidase C19
                     contains ubiquitinyl hydrolases. They are intracellular
                     peptidases that remove ubiquitin molecules from
                     polyubiquinated peptides by cleavage of isopeptide bonds.
                     They hydrolyze bonds involving the carboxyl...; cd02674"
                     /db_xref="CDD:239139"
     CDS             1..1050
                     /gene="LOC117607366"
                     /coded_by="XM_034330986.2:176..3328"
                     /db_xref="GeneID:117607366"
ORIGIN      
        1 mdnkvdvsev edismigsse ifylpevaei sevldstgls pltssldhtl tlqedkllss
       61 evnmgiedtw teansstsdy aiplppppgl ndftdvgadp isdsgtgieh gpflppgtpa
      121 lnnlfseggd sqddspmedv vlragvcglr nlgntcfmaa glqcltatpp vlrhfldlqq
      181 kgeklpppgs lmahfsvllg kmwsgrysvl rptefkqtlg ayhsqfkdyr qhdcqeflal
      241 lldslheqmn takytkncqt psstttansi nsiensngkt vssvtansns ncntdllemy
      301 dclpspecpn spnvtmagsp rdlgspigei dsimnspres plndgdedee tldsdeviee
      361 ktntfihnkv dekesevsps lrldtliels knvpkyhgly dihkeaktsn anflvtqqec
      421 nneihydsqk fpkdnvrrmp ldnsnlmenh dfdnksisvk rikevniqkv ncgvdyvssg
      481 sdieydngle kcnvkrmrld dqeknhkrdg qgsissqctr isqsfgngai aaqdeieadk
      541 hwakhlrsnr siivdtfqgq fkskvvcavc khvsvtyepf mylsvplpha merqlnvtyv
      601 pangdqpirc vvslnkqsri gklkeellki lgkeniaian ialaevlenh iskilddntl
      661 lrhindtnrs iyafeltepp daytsvpdgg gdrvmdtdnc qkcavtgeei qcmicleeld
      721 gglkrhsgns cnfvicdtci enyfknqtep qmcsvcstym tassfvkidq tgrpairiln
      781 vplvlrqdtt nettnnrkgt klfgyphlvk lpsrvnakdl ydivkrnvpq egnytlhfvt
      841 gqghhcsrci ytahctgcsv pesgvvilht gdtlavryte hvpkiappid hisvskqrph
      901 hplslydclq afsqsetlde hnpwfcpkce rnqcatktlt vhrypkfliv ylkrfvfyec
      961 asmklddkvt fplvglsvgq hlydlyacvc hfggvsaghy tayaknprtd vwyyyndeit
     1021 srqkpqdedf snayilfysr qgtnlkpcni
//
LOCUS       XP_034186878             935 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607366 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186878
VERSION     XP_034186878.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330987.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186878.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..935
                     /product="uncharacterized protein LOC117607366 isoform X2"
                     /calculated_mol_wt=103433
     Region          147..>289
                     /region_name="UCH"
                     /note="Ubiquitin carboxyl-terminal hydrolase; pfam00443"
                     /db_xref="CDD:425685"
     Region          <538..>678
                     /region_name="UBP12"
                     /note="Ubiquitin C-terminal hydrolase [Posttranslational
                     modification, protein turnover, chaperones]; COG5560"
                     /db_xref="CDD:227847"
     CDS             1..935
                     /gene="LOC117607366"
                     /coded_by="XM_034330987.2:408..3215"
                     /db_xref="GeneID:117607366"
ORIGIN      
        1 mdnkvdvsev edismigsse ifylpevaei sevldstgls pltssldhtl tlqedkllss
       61 evnmgiedtw teansstsdy aiplppppgl ndftdvgadp isdsgtgieh gpflppgtpa
      121 lnnlfseggd sqddspmedv vlragvcglr nlgntcfmaa glqcltatpp vlrhfldlqq
      181 kgeklpppgs lmahfsvllg kmwsgrysvl rptefkqtlg ayhsqfkdyr qhdcqeflal
      241 lldslheqmn takytkncqt psstttansi nsiensngkt vssvtansns ncntdllemy
      301 dclpspecpn spnvtmagsp rdlgspigei dsimnspres plndgdedee tldsdeviee
      361 ktntfihnkv dekesevsps lrldtliels knvpkyhgly dihkeaktsn anflvtqqec
      421 nneihydsqk fpkdnvrrmp ldnsnlmenh dfdnksisvk rikevniqkv ncgvdyvssg
      481 sdieydngle kcnvkrmrld dqeknhkrdg qgsissqctr isqsfgngai aaqdeieadk
      541 hwakhlrsnr siivdtfqgq fkskvvcavc khvsvtyepf mylsvplpha merqlnvtyv
      601 pangdqpirc vvslnkqsri gklkeellki lgkeniaian ialaevlenh iskilddntl
      661 lrhindtnrs iyafeltepp daytsvpdgg gdrvmdtdnc qkcavtgeei qcmicleeld
      721 gglkrhsgns cnfvicdtci enyfknqtep qmcsvcstym tassfvkidq tgrpairiln
      781 vplvlrqdtt nettnnrkgt klfgyphlvk lpsrvnakdl ydivkrnvpq egnytlhfvt
      841 gqghhcsrci ytahctgcsv pesgvvilht gdtlavryte hvpkiappid hisvskqrph
      901 hplslydclq afsqsasein vlqkhsrfid ipsss
//
LOCUS       XP_034186881             895 aa            linear   INV 03-SEP-2025
DEFINITION  follistatin-like 5 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186881
VERSION     XP_034186881.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330990.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186881.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..895
                     /product="follistatin-like 5 isoform X1"
                     /calculated_mol_wt=101145
     Region          107..147
                     /region_name="KAZAL_FS"
                     /note="Kazal type serine protease inhibitors and
                     follistatin-like domains. Kazal inhibitors inhibit serine
                     proteases, such as, trypsin, chyomotrypsin, avian
                     ovomucoids, and elastases. The inhibitory domain has one
                     reactive site peptide bond, which serves the...; cd00104"
                     /db_xref="CDD:238052"
     Region          238..298
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(243,245,247,254,281,283,285,292)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          316..392
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          325..329
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          338..342
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          362..366
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          376..381
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          399..491
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          416..420
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          429..433
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          454..458
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          468..473
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          483..486
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <548..630
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     CDS             1..895
                     /gene="Fstl5"
                     /coded_by="XM_034330990.2:328..3015"
                     /db_xref="GeneID:117607367"
ORIGIN      
        1 mmkrkfqiat lyllllsicm svaghkhsrr hrdftvaenh dsaslsrsds hsmtvsplie
       61 rsslpeeayl petptvdpcv kkycgvgrec eqsanntvat cvctrkcprr hrpvcasngk
      121 iyanhcelhr aachagsslt rsrlmrclhh dienahvrrt lhvegtsskt gktityprfr
      181 nrkktnlrdn lipeknnsdt kecsnqeyei mkdnlllynh arlmaqdnhs keylvsimfs
      241 hydqnnngnl ereeleqiae nedleelckg cnlghminyd dtdgdgklnv nefymafskl
      301 ysvsvvsldk slevnhisar vgdnveikcd vtgtpppplv wrrneadlei lnepeirvfn
      361 dgslyltkvq lihagnytch avrnqdvvqt hvltihtvpe vkvtpqfqak rlkeeasikc
      421 hvtgeplprv ewlkndepln hdqpdkydli gngtklmikn vdyadtgaym cqassiggvt
      481 rdisslvvqd qptpttenee rrffsfhkwg ilvyepstcr lrheirstdv ipgtqeyvcg
      541 iknvpcswgr ainvanryvy vsqpqkdrvl visetqmviv dvvptdknpv qlwyvpsldq
      601 vwvlnwrsee dtgvktvqvi edasqkkkhr airpepidaq fdiiqniyip epqdidstfk
      661 ygyvshtnqr nmykldlknm kytrsadlss ydcvpanvqf silygfviie cqepitnrpt
      721 gqllldyltd tvlvhkpnll grptispdsr hlvtldkqet gvtlvvqeis snglkfafdv
      781 kttlnisdia lypsqtthgy diyassidke dilfldlstg kvemitgvgk atppnltkwg
      841 npnrpivqsg ifgkymvsps sdalfvlnge trtinceigg lvnpgavvwf tvslh
//
LOCUS       XP_076548215             894 aa            linear   INV 03-SEP-2025
DEFINITION  follistatin-like 5 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548215
VERSION     XP_076548215.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692100.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..894
                     /product="follistatin-like 5 isoform X2"
                     /calculated_mol_wt=101014
     Region          106..146
                     /region_name="KAZAL_FS"
                     /note="Kazal type serine protease inhibitors and
                     follistatin-like domains. Kazal inhibitors inhibit serine
                     proteases, such as, trypsin, chyomotrypsin, avian
                     ovomucoids, and elastases. The inhibitory domain has one
                     reactive site peptide bond, which serves the...; cd00104"
                     /db_xref="CDD:238052"
     Region          237..297
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(242,244,246,253,280,282,284,291)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          315..391
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          324..328
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          337..341
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          361..365
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          375..380
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          398..490
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          415..419
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          428..432
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          453..457
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          467..472
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          482..485
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <547..629
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     CDS             1..894
                     /gene="Fstl5"
                     /coded_by="XM_076692100.1:498..3182"
                     /db_xref="GeneID:117607367"
ORIGIN      
        1 mkrkfqiatl yllllsicms vaghkhsrrh rdftvaenhd saslsrsdsh smtvsplier
       61 sslpeeaylp etptvdpcvk kycgvgrece qsanntvatc vctrkcprrh rpvcasngki
      121 yanhcelhra achagssltr srlmrclhhd ienahvrrtl hvegtssktg ktityprfrn
      181 rkktnlrdnl ipeknnsdtk ecsnqeyeim kdnlllynha rlmaqdnhsk eylvsimfsh
      241 ydqnnngnle reeleqiaen edleelckgc nlghminydd tdgdgklnvn efymafskly
      301 svsvvsldks levnhisarv gdnveikcdv tgtpppplvw rrneadleil nepeirvfnd
      361 gslyltkvql ihagnytcha vrnqdvvqth vltihtvpev kvtpqfqakr lkeeasikch
      421 vtgeplprve wlkndeplnh dqpdkydlig ngtklmiknv dyadtgaymc qassiggvtr
      481 disslvvqdq ptptteneer rffsfhkwgi lvyepstcrl rheirstdvi pgtqeyvcgi
      541 knvpcswgra invanryvyv sqpqkdrvlv isetqmvivd vvptdknpvq lwyvpsldqv
      601 wvlnwrseed tgvktvqvie dasqkkkhra irpepidaqf diiqniyipe pqdidstfky
      661 gyvshtnqrn mykldlknmk ytrsadlssy dcvpanvqfs ilygfviiec qepitnrptg
      721 qllldyltdt vlvhkpnllg rptispdsrh lvtldkqetg vtlvvqeiss nglkfafdvk
      781 ttlnisdial ypsqtthgyd iyassidked ilfldlstgk vemitgvgka tppnltkwgn
      841 pnrpivqsgi fgkymvspss dalfvlnget rtinceiggl vnpgavvwft vslh
//
LOCUS       XP_034186882             817 aa            linear   INV 03-SEP-2025
DEFINITION  follistatin-like 5 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186882
VERSION     XP_034186882.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330991.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186882.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..817
                     /product="follistatin-like 5 isoform X3"
                     /calculated_mol_wt=92510
     Region          160..220
                     /region_name="EFh"
                     /note="EF-hand, calcium binding motif; A diverse
                     superfamily of calcium sensors and calcium signal
                     modulators; most examples in this alignment model have 2
                     active canonical EF hands. Ca2+ binding induces a
                     conformational change in the EF-hand motif, leading to...;
                     cd00051"
                     /db_xref="CDD:238008"
     Site            order(165,167,169,176,203,205,207,214)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238008"
     Region          238..314
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          247..251
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          260..264
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          284..288
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          298..303
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          321..413
                     /region_name="Ig"
                     /note="Immunoglobulin domain; cl11960"
                     /db_xref="CDD:472250"
     Region          338..342
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          351..355
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          376..380
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          390..395
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          405..408
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          <470..552
                     /region_name="YncE"
                     /note="DNA-binding beta-propeller fold protein YncE
                     [General function prediction only]; COG3391"
                     /db_xref="CDD:442618"
     CDS             1..817
                     /gene="Fstl5"
                     /coded_by="XM_034330991.2:328..2781"
                     /db_xref="GeneID:117607367"
ORIGIN      
        1 mmkrkfqiat lyllllsicm svaghkhsrr hrdftvaenh dsaslsrsds hsmtvsplie
       61 rsslpeeayl penienahvr rtlhvegtss ktgktitypr frnrkktnlr dnlipeknns
      121 dtkecsnqey eimkdnllly nharlmaqdn hskeylvsim fshydqnnng nlereeleqi
      181 aenedleelc kgcnlghmin yddtdgdgkl nvnefymafs klysvsvvsl dkslevnhis
      241 arvgdnveik cdvtgtpppp lvwrrneadl eilnepeirv fndgslyltk vqlihagnyt
      301 chavrnqdvv qthvltihtv pevkvtpqfq akrlkeeasi kchvtgeplp rvewlkndep
      361 lnhdqpdkyd ligngtklmi knvdyadtga ymcqassigg vtrdisslvv qdqptptten
      421 eerrffsfhk wgilvyepst crlrheirst dvipgtqeyv cgiknvpcsw grainvanry
      481 vyvsqpqkdr vlvisetqmv ivdvvptdkn pvqlwyvpsl dqvwvlnwrs eedtgvktvq
      541 viedasqkkk hrairpepid aqfdiiqniy ipepqdidst fkygyvshtn qrnmykldlk
      601 nmkytrsadl ssydcvpanv qfsilygfvi iecqepitnr ptgqllldyl tdtvlvhkpn
      661 llgrptispd srhlvtldkq etgvtlvvqe issnglkfaf dvkttlnisd ialypsqtth
      721 gydiyassid kedilfldls tgkvemitgv gkatppnltk wgnpnrpivq sgifgkymvs
      781 pssdalfvln getrtincei gglvnpgavv wftvslh
//
LOCUS       XP_034186883             546 aa            linear   INV 03-SEP-2025
DEFINITION  fatty-acid amide hydrolase 2-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186883
VERSION     XP_034186883.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330992.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186883.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..546
                     /product="fatty-acid amide hydrolase 2-like isoform X1"
                     /calculated_mol_wt=61051
     Region          67..540
                     /region_name="GatA"
                     /note="Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit
                     or related amidase [Translation, ribosomal structure and
                     biogenesis]; COG0154"
                     /db_xref="CDD:439924"
     CDS             1..546
                     /gene="LOC117607368"
                     /coded_by="XM_034330992.2:838..2478"
                     /db_xref="GeneID:117607368"
ORIGIN      
        1 melllcalpl vytkimvhrw dtvilivqki lkfllfllyc lvtpflmlqc lkkrkrlppi
       61 nshvlllsat eiarrirnrk isseevvrac verckdvnpi lnaivdsrfd eaiqearkvd
      121 efllwsrkse eelarempll gvpmtvkesi avqgmsysvg vkkkipdkas qdatvvemvr
      181 eagaiillvs ntpelclfwe snnkvtgptw npydttkvag gssggeaall gsgasilsls
      241 sdiagsarlp amfcgifghk ptaclvsckg hkpdsndrnw pyyftigtmv ryaedlslmm
      301 kiisqseear nrfdqkvsle dmkffyledc cgitnsidre mkgaiqklrk hlettygltv
      361 qkaqlndmkf afdiathlll emevdgitee vkavgsskil lellkcfffa sshslppisy
      421 gllkwlsskf pnsyrskmvd kkmalkkqfe dllgdngvli yptyvtpahy hhqsypkvan
      481 ftymmiynvl glpvtqcpmg lnskglpigl qivanagndh ltiavaqrie kafggwqqpp
      541 ttelsi
//
LOCUS       XP_034186884             527 aa            linear   INV 03-SEP-2025
DEFINITION  fatty-acid amide hydrolase 2-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186884
VERSION     XP_034186884.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330993.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186884.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..527
                     /product="fatty-acid amide hydrolase 2-like isoform X2"
                     /calculated_mol_wt=58770
     Region          48..521
                     /region_name="GatA"
                     /note="Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit
                     or related amidase [Translation, ribosomal structure and
                     biogenesis]; COG0154"
                     /db_xref="CDD:439924"
     CDS             1..527
                     /gene="LOC117607368"
                     /coded_by="XM_034330993.2:432..2015"
                     /db_xref="GeneID:117607368"
ORIGIN      
        1 mdtvilivqk ilkfllflly clvtpflmlq clkkrkrlpp inshvlllsa teiarrirnr
       61 kisseevvra cverckdvnp ilnaivdsrf deaiqearkv defllwsrks eeelarempl
      121 lgvpmtvkes iavqgmsysv gvkkkipdka sqdatvvemv reagaiillv sntpelclfw
      181 esnnkvtgpt wnpydttkva ggssggeaal lgsgasilsl ssdiagsarl pamfcgifgh
      241 kptaclvsck ghkpdsndrn wpyyftigtm vryaedlslm mkiisqseea rnrfdqkvsl
      301 edmkffyled ccgitnsidr emkgaiqklr khlettyglt vqkaqlndmk fafdiathll
      361 lemevdgite evkavgsski llellkcfff asshslppis ygllkwlssk fpnsyrskmv
      421 dkkmalkkqf edllgdngvl iyptyvtpah yhhqsypkva nftymmiynv lglpvtqcpm
      481 glnskglpig lqivanagnd hltiavaqri ekafggwqqp pttelsi
//
LOCUS       XP_034186885             527 aa            linear   INV 03-SEP-2025
DEFINITION  fatty-acid amide hydrolase 2-like isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186885
VERSION     XP_034186885.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330994.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186885.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..527
                     /product="fatty-acid amide hydrolase 2-like isoform X3"
                     /calculated_mol_wt=58770
     Region          48..521
                     /region_name="GatA"
                     /note="Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit
                     or related amidase [Translation, ribosomal structure and
                     biogenesis]; COG0154"
                     /db_xref="CDD:439924"
     CDS             1..527
                     /gene="LOC117607368"
                     /coded_by="XM_034330994.2:124..1707"
                     /db_xref="GeneID:117607368"
ORIGIN      
        1 mdtvilivqk ilkfllflly clvtpflmlq clkkrkrlpp inshvlllsa teiarrirnr
       61 kisseevvra cverckdvnp ilnaivdsrf deaiqearkv defllwsrks eeelarempl
      121 lgvpmtvkes iavqgmsysv gvkkkipdka sqdatvvemv reagaiillv sntpelclfw
      181 esnnkvtgpt wnpydttkva ggssggeaal lgsgasilsl ssdiagsarl pamfcgifgh
      241 kptaclvsck ghkpdsndrn wpyyftigtm vryaedlslm mkiisqseea rnrfdqkvsl
      301 edmkffyled ccgitnsidr emkgaiqklr khlettyglt vqkaqlndmk fafdiathll
      361 lemevdgite evkavgsski llellkcfff asshslppis ygllkwlssk fpnsyrskmv
      421 dkkmalkkqf edllgdngvl iyptyvtpah yhhqsypkva nftymmiynv lglpvtqcpm
      481 glnskglpig lqivanagnd hltiavaqri ekafggwqqp pttelsi
//
LOCUS       XP_034186887             520 aa            linear   INV 03-SEP-2025
DEFINITION  fatty-acid amide hydrolase 2-like [Osmia lignaria lignaria].
ACCESSION   XP_034186887
VERSION     XP_034186887.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330996.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186887.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..520
                     /product="fatty-acid amide hydrolase 2-like"
                     /calculated_mol_wt=57837
     Region          42..514
                     /region_name="GatA"
                     /note="Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit
                     or related amidase [Translation, ribosomal structure and
                     biogenesis]; COG0154"
                     /db_xref="CDD:439924"
     CDS             1..520
                     /gene="LOC117607369"
                     /coded_by="XM_034330996.2:352..1914"
                     /db_xref="GeneID:117607369"
ORIGIN      
        1 mndfvakfcv vnfhlihfii rpfyaliyrn kprdipsikn pllklsatml arkirnhels
       61 sqtvveayie rikevdpfin avieerfeaa lneaktcdek lkneeiiald lekekplygv
      121 pvtikeslsv kgmsitggci krknfkaeed gdavklikna gaivllvsnt selctainsy
      181 nhlfgqtknp ynrtrtsggs sggegalita gasvlgfgsd vvgsiripal wngifghkpt
      241 pgiipvkghy ptmddelfqn flvlgplary aedlqlamkv ftsrlekplc fdeplnlknl
      301 rvfyvdnfds fcntrstssd iketihkals ffakngayve hlsqewvadi fnielsafge
      361 ldipellvdp knpegkrrpf lefaksvvrl sdytpsltlv rivieihggi trsgiekykk
      421 irnelrqkvn nmlkddavfi yptfpsaaty pelvffnfds sayaslanll hlpsthipmg
      481 lnndglpigf qvtaasyqdh lsiavakele kafggwipps
//
LOCUS       XP_034186889             343 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607370 [Osmia lignaria lignaria].
ACCESSION   XP_034186889
VERSION     XP_034186889.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330998.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186889.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..343
                     /product="uncharacterized protein LOC117607370"
                     /calculated_mol_wt=39564
     Region          69..190
                     /region_name="DUF4497"
                     /note="Protein of unknown function (DUF4497); pfam14924"
                     /db_xref="CDD:464380"
     CDS             1..343
                     /gene="LOC117607370"
                     /coded_by="XM_034330998.2:755..1786"
                     /db_xref="GeneID:117607370"
ORIGIN      
        1 mssltvvhds rlmllelmvq nlympwkdil datlmkktvi mfrmldnewt tlmpnrrdyr
       61 syrgsnyqne rfyggrsvvf alpestlekn vsgvdlqlyv skevskyfel errqnfgftl
      121 vkmdgllngi ikdlrernel egyfssaler episrstrgt fplfdedvqk tnatielyvr
      181 isflgkciit evyapistkk afyareesde rypyqfrelt tvdvesfcwg sltiippvhp
      241 dyltcackii pdsfeettqt kkkkrkqkpt lkeiylarla lfqellaklk elrknrpeii
      301 epggkpkgic pppcrpisci ytlppsvcts pcqptvycla nat
//
LOCUS       XP_034186890             333 aa            linear   INV 03-SEP-2025
DEFINITION  acyl-CoA Delta-9 desaturase isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186890
VERSION     XP_034186890.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330999.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186890.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="acyl-CoA Delta-9 desaturase isoform X1"
                     /calculated_mol_wt=38569
     Region          68..306
                     /region_name="Delta9-FADS-like"
                     /note="The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like
                     CD includes the delta-9 and delta-11 acyl CoA desaturases
                     found in various eukaryotes including vertebrates,
                     insects, higher plants, and fungi. The delta-9 acyl-lipid
                     desaturases are found in a wide...; cd03505"
                     /db_xref="CDD:239582"
     Site            order(89,94,126,129..130,266,269..270)
                     /site_type="other"
                     /note="Di-iron ligands [ion binding]"
                     /db_xref="CDD:239582"
     CDS             1..333
                     /gene="LOC117607371"
                     /coded_by="XM_034330999.2:251..1252"
                     /db_xref="GeneID:117607371"
ORIGIN      
        1 mysstvtqye ekkevngkve vdveplalpa eessasqpii wrnvigiivl hflpvygfvn
       61 gyrdakfwtw iwsiayglva gvgvtagahr lwahrsysak vplriflayl ycmagqthfy
      121 kwirdhrthh rytettadph darrgfffsh igwlmvkrhp svkkygskid mtdvasdsvi
      181 kfydkyyeii mvwlcfvqpi llpvfmwget wfisihatli ryvwslhatf ivnsfahmwg
      241 nrpynrrvkp tenaavsffa lgegwhnyhh sfpwdykaae lgayslnptt afielmarvg
      301 faynlktpsk vlvdrtimkk gdgtdslwgh qrh
//
LOCUS       XP_034186891             272 aa            linear   INV 03-SEP-2025
DEFINITION  acyl-CoA Delta-9 desaturase isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186891
VERSION     XP_034186891.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331000.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186891.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..272
                     /product="acyl-CoA Delta-9 desaturase isoform X2"
                     /calculated_mol_wt=31107
     Region          68..245
                     /region_name="Delta9-FADS-like"
                     /note="The Delta9 Fatty Acid Desaturase (Delta9-FADS)-like
                     CD includes the delta-9 and delta-11 acyl CoA desaturases
                     found in various eukaryotes including vertebrates,
                     insects, higher plants, and fungi. The delta-9 acyl-lipid
                     desaturases are found in a wide...; cd03505"
                     /db_xref="CDD:239582"
     Site            order(89,94,126,129..130,205,208..209)
                     /site_type="other"
                     /note="Di-iron ligands [ion binding]"
                     /db_xref="CDD:239582"
     CDS             1..272
                     /gene="LOC117607371"
                     /coded_by="XM_034331000.2:250..1068"
                     /db_xref="GeneID:117607371"
ORIGIN      
        1 mysstvtqye ekkevngkve vdveplalpa eessasqpii wrnvigiivl hflpvygfvn
       61 gyrdakfwtw iwsiayglva gvgvtagahr lwahrsysak vplriflayl ycmagqthfy
      121 kwirdhrthh rytettadph darrgfffsh igwlmvkrhp svkkygskid mtdvasdsvi
      181 kfydkrvkpt enaavsffal gegwhnyhhs fpwdykaael gayslnptta fielmarvgf
      241 aynlktpskv lvdrtimkkg dgtdslwghq rh
//
LOCUS       XP_076548216             227 aa            linear   INV 03-SEP-2025
DEFINITION  hemolymph lipopolysaccharide-binding protein-like isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076548216
VERSION     XP_076548216.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692101.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..227
                     /product="hemolymph lipopolysaccharide-binding
                     protein-like isoform X1"
                     /calculated_mol_wt=25563
     Region          95..221
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(195,199,201,207..210,213..215)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..227
                     /gene="LOC143305233"
                     /coded_by="XM_076692101.1:207..890"
                     /db_xref="GeneID:143305233"
ORIGIN      
        1 msrfsilvnc iilcvafrgt fsmleklavl kinetenili ddtscpchrh slksywnelp
       61 tsnadvlgtq cvcdlgvrtk hlhpdywysh gigmhklhtr aetwntarvi cteegghlav
      121 vnsaeeayim aqlfrvyrpf iganriyeaf vgmhdiykeg hwttvlgdpl ketgytkwsn
      181 ymrpqpdnga gakvencggi iwdatlddin cdtpfaffce ipeitpl
//
LOCUS       XP_076548217             168 aa            linear   INV 03-SEP-2025
DEFINITION  hemolymph lipopolysaccharide-binding protein-like isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076548217
VERSION     XP_076548217.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692102.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..168
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..168
                     /product="hemolymph lipopolysaccharide-binding
                     protein-like isoform X2"
                     /calculated_mol_wt=18852
     Region          36..162
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(136,140,142,148..151,154..156)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..168
                     /gene="LOC143305233"
                     /coded_by="XM_076692102.1:209..715"
                     /db_xref="GeneID:143305233"
ORIGIN      
        1 msrnadvlgt qcvcdlgvrt khlhpdywys hgigmhklht raetwntarv icteegghla
       61 vvnsaeeayi maqlfrvyrp figanriyea fvgmhdiyke ghwttvlgdp lketgytkws
      121 nymrpqpdng agakvencgg iiwdatlddi ncdtpfaffc eipeitpl
//
LOCUS       XP_034186982             225 aa            linear   INV 03-SEP-2025
DEFINITION  hemolymph lipopolysaccharide-binding protein-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034186982
VERSION     XP_034186982.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331091.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186982.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..225
                     /product="hemolymph lipopolysaccharide-binding
                     protein-like"
                     /calculated_mol_wt=24958
     Region          95..219
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(193,197,199,203,205..208,211..213)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..225
                     /gene="LOC117607421"
                     /coded_by="XM_034331091.2:159..836"
                     /db_xref="GeneID:117607421"
ORIGIN      
        1 msalsilvnc iilcvafrgt fsipkeitap thkvnktkhi hetsipsphh plslnrdkyr
       61 srnmmvhgtv cmcnlpvhgt pprddykytp eigshkfhtr aqpwnkarei cneegghlai
      121 insyaeqrvl mdlfnhsgpv igsgyedeaf lgihdlyaeg dwvtilgdpl aktgfdlwsd
      181 kwggqpdneg gvqncgallm dgtmddikcd vpfgffceip elqpl
//
LOCUS       XP_034186892             225 aa            linear   INV 03-SEP-2025
DEFINITION  hemolymph lipopolysaccharide-binding protein-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034186892
VERSION     XP_034186892.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331001.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186892.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..225
                     /product="hemolymph lipopolysaccharide-binding
                     protein-like"
                     /calculated_mol_wt=25400
     Region          95..219
                     /region_name="CLECT"
                     /note="C-type lectin (CTL)/C-type lectin-like (CTLD)
                     domain; cd00037"
                     /db_xref="CDD:153057"
     Site            order(192,196,198,203,205..208,211..213)
                     /site_type="other"
                     /note="ligand binding surface [chemical binding]"
                     /db_xref="CDD:153057"
     CDS             1..225
                     /gene="LOC117607372"
                     /coded_by="XM_034331001.2:162..839"
                     /db_xref="GeneID:117607372"
ORIGIN      
        1 msqlsflvnc iilcvafrgi fsvpkkvpvl kinetenili hhtscpcdrd plelywdklh
       61 trnvnvqstv cacnlgmrgk pprddyiyap gigyhkmypt getwntarki cnaeggqlai
      121 insyaealal trlfsiyfpi qdaprndtmf lgihdlysfr dwttvlgepl telgypssyl
      181 qhrqwykesg qyhcggynvg adlmtpvncs lphaffceip eitpl
//
LOCUS       XP_034186897             217 aa            linear   INV 03-SEP-2025
DEFINITION  MOB kinase activator-like 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186897
VERSION     XP_034186897.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331006.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="MOB kinase activator-like 1 isoform X1"
                     /calculated_mol_wt=24923
     Region          34..204
                     /region_name="Mob1_phocein"
                     /note="Mob1/phocein family; pfam03637"
                     /db_xref="CDD:461000"
     CDS             1..217
                     /gene="mats"
                     /coded_by="XM_034331006.2:391..1044"
                     /db_xref="GeneID:117607376"
ORIGIN      
        1 msflfgsrss ktfkpkknip egthqydlmk haaatlgsgn lrlavmlpeg edlnewvavn
       61 tvdffnqinm lygtitefct eescpimsag pkyeyhwadg htvkkpikcs apkyidylmt
      121 wvqdqlddet lfpskigvpf pknflsiakt ilkrlfrvya hiyhqhfsev vqlgeeahln
      181 tsfkhfiffv qefnlierre laplqeliek ltakdar
//
LOCUS       XP_034186898             217 aa            linear   INV 03-SEP-2025
DEFINITION  MOB kinase activator-like 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186898
VERSION     XP_034186898.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331007.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..217
                     /product="MOB kinase activator-like 1 isoform X2"
                     /calculated_mol_wt=24890
     Region          34..204
                     /region_name="Mob1_phocein"
                     /note="Mob1/phocein family; pfam03637"
                     /db_xref="CDD:461000"
     CDS             1..217
                     /gene="mats"
                     /coded_by="XM_034331007.2:16..669"
                     /db_xref="GeneID:117607376"
ORIGIN      
        1 mnflsgsrss ktfkpkknip egthqydlmk haaatlgsgn lrlavmlpeg edlnewvavn
       61 tvdffnqinm lygtitefct eescpimsag pkyeyhwadg htvkkpikcs apkyidylmt
      121 wvqdqlddet lfpskigvpf pknflsiakt ilkrlfrvya hiyhqhfsev vqlgeeahln
      181 tsfkhfiffv qefnlierre laplqeliek ltakdar
//
LOCUS       XP_034186732             389 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607297 [Osmia lignaria lignaria].
ACCESSION   XP_034186732
VERSION     XP_034186732.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330841.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="uncharacterized protein LOC117607297"
                     /calculated_mol_wt=44514
     Region          43..73
                     /region_name="WW"
                     /note="Two conserved tryptophans domain; also known as the
                     WWP or rsp5 domain; around 40 amino acids; functions as an
                     interaction module in a diverse set of signalling
                     proteins; binds specific proline-rich sequences but at low
                     affinities compared to other...; cd00201"
                     /db_xref="CDD:238122"
     Site            order(58,69)
                     /site_type="active"
                     /note="binding pocket [active]"
                     /db_xref="CDD:238122"
     CDS             1..389
                     /gene="LOC117607297"
                     /coded_by="XM_034330841.1:108..1277"
                     /db_xref="GeneID:117607297"
ORIGIN      
        1 msscdsysfk ssdklsvfdk tdnakksvnv dqksefgkye etynwveces mqfpgkpyff
       61 nlrtqnttwi rpvsrhvqlq rfsrkskifd dnstpekeip lilssesekd dweyidneen
      121 nqsnkeiykn seilarlyyg tklnqfsnnd etlyfhdyfn yfvenlsnkd sqgshevaee
      181 etvcdksale nspiiisnfl dvelsvseda lnikenieps nednisliek piiystsssd
      241 eekkennmre nyivprlkki kplcllkksf knakrkkvak kriplqekkl qevkivremy
      301 gtttisydpv epelppgvrk rnlsdsssee envrsvwnns klfnidsyva nlssksssss
      361 ssnssssssc sctcsccsss sttsktskh
//
LOCUS       XP_034186476            4342 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186476
VERSION     XP_034186476.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330585.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186476.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4342
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X1"
                     /calculated_mol_wt=485264
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2737..2790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2738,2778..2779,2781..2782,2784..2786,2788..2790)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2907..>3176
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3685..3728
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4033..4186
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4342
                     /gene="kis"
                     /coded_by="XM_034330585.2:462..13490"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnsssrddt
     2581 slendiedae sigmmkdskd sdkfsretpt dspaisskad tpgpepsssh egndglldde
     2641 kawpsvvdln trlrrvissy qrsvkkedvk vlqksktgme setsinhnsp mnepplnmqg
     2701 wdlqqlamyl lkmerrekme amvkerertr ieeqktkwsr reeseflrvv stygvnydrk
     2761 kaqydwtkfk siarfdkktd ndltdyymsf ramckkacna klndeddfsd vavdhlspak
     2821 arqildrvdl lskireeils hphleerlsl cqpsgdtpdw wqlgkhdkel llgaakhglg
     2881 rtditilndp dfsfhkllnk nmysniqtsk itdksekaik ienredilkf dkdeilvkle
     2941 kgegtlkiek vgakkdkdin vtdkrtentd seksqvelti ikaenkveek pisnaltitt
     3001 varststdkd hipkvkteek seiviesikn epsktdqimg tnktdevkas nvirsaeetv
     3061 ttikteienq tkekeapkvv ekenqdeksk elqtqdtske tnekssteqk entegtekst
     3121 ennedvtpes dkvnvnavtv keeievqegk ttdtckdkak vckledkcnv qaaelkamfp
     3181 dleviqplsr ltqidtfvlr dkaidynept vvqllshtsn navkwpkeha iearlmhivh
     3241 aiehkewpvs vnfsagdesd ipsnekecse vitittdhgm srnttpgnnv sqskkrkrhi
     3301 aidveterak lhallnsssh tvtqspatls kptvlsgsnt wemndespse esrrstpvqp
     3361 ppahqqartp sipfdlkytv pgkptvipgt sstltpidls agypktstdn nkdimnevqd
     3421 fsmpnknkqi ssnkgkldsm ldklmkrknc ptdepvmgke kkrrkldeiv lglsaakeqq
     3481 sshypehskk stitpnvtvt ptsapmglpn ppsstqkpfs itvtsipssr asnstsglpp
     3541 pslhshkdsf sallvqaeqq nrefkkqqqh qqqsqqqqsq qqqqqqqqqq qqqqqqqqqq
     3601 qqqnfnsths ssssghrksy eamiadinkv aeysskigsy sheakvnkwl aeqtamseql
     3661 gaeylnaprr rrprvdpsll dwkkltgeen vavinrltgk kvtgskapql krlgqwlien
     3721 pmfdvdpkwa elvkergnlp hdlqkrlsgn drsnankgks pgrppmmpsp nsqqsanqan
     3781 laatsmasgl nfsslgnlnn sllsglslgn fdpknnpllm pfgglpnlga lsglgnlnnl
     3841 snmsltnslf anlaglglps lagmeaalsa tatstaaetn pvvssvsskn tgstnissas
     3901 kssrnklepp tskslvpsts sasstipttt pfpfffpnps llytplglgg lnpfsmqpgg
     3961 vssayeslaq cgllnpstss astvrkptss asnsrqrdti testtkrkda ekksrysidp
     4021 gitlqqytdm tlqheerrki eqekrenleq ndvadlsdrr terknkeqem kealehlsrt
     4081 saelltrnle dqrsnekrsr nvehsqgseq qtsgmleitl epinkkprid ievsskpvts
     4141 tattpitsad gstgetehaa rssvvtvkqs cieesntfse qssntnkket detvatitti
     4201 stptitvtva statptstpt rtptpistpt stptstptst siitptstts vtigsedaat
     4261 sqsstnnakg dtsklsseve ednkgtkgkk arsgkrlsve ppmerknlrs sagrqaraaa
     4321 erqarmegel qsdsqethtt te
//
LOCUS       XP_034186477            4342 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186477
VERSION     XP_034186477.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330586.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186477.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4342
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X1"
                     /calculated_mol_wt=485264
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2737..2790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2738,2778..2779,2781..2782,2784..2786,2788..2790)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2907..>3176
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3685..3728
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4033..4186
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4342
                     /gene="kis"
                     /coded_by="XM_034330586.2:679..13707"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnsssrddt
     2581 slendiedae sigmmkdskd sdkfsretpt dspaisskad tpgpepsssh egndglldde
     2641 kawpsvvdln trlrrvissy qrsvkkedvk vlqksktgme setsinhnsp mnepplnmqg
     2701 wdlqqlamyl lkmerrekme amvkerertr ieeqktkwsr reeseflrvv stygvnydrk
     2761 kaqydwtkfk siarfdkktd ndltdyymsf ramckkacna klndeddfsd vavdhlspak
     2821 arqildrvdl lskireeils hphleerlsl cqpsgdtpdw wqlgkhdkel llgaakhglg
     2881 rtditilndp dfsfhkllnk nmysniqtsk itdksekaik ienredilkf dkdeilvkle
     2941 kgegtlkiek vgakkdkdin vtdkrtentd seksqvelti ikaenkveek pisnaltitt
     3001 varststdkd hipkvkteek seiviesikn epsktdqimg tnktdevkas nvirsaeetv
     3061 ttikteienq tkekeapkvv ekenqdeksk elqtqdtske tnekssteqk entegtekst
     3121 ennedvtpes dkvnvnavtv keeievqegk ttdtckdkak vckledkcnv qaaelkamfp
     3181 dleviqplsr ltqidtfvlr dkaidynept vvqllshtsn navkwpkeha iearlmhivh
     3241 aiehkewpvs vnfsagdesd ipsnekecse vitittdhgm srnttpgnnv sqskkrkrhi
     3301 aidveterak lhallnsssh tvtqspatls kptvlsgsnt wemndespse esrrstpvqp
     3361 ppahqqartp sipfdlkytv pgkptvipgt sstltpidls agypktstdn nkdimnevqd
     3421 fsmpnknkqi ssnkgkldsm ldklmkrknc ptdepvmgke kkrrkldeiv lglsaakeqq
     3481 sshypehskk stitpnvtvt ptsapmglpn ppsstqkpfs itvtsipssr asnstsglpp
     3541 pslhshkdsf sallvqaeqq nrefkkqqqh qqqsqqqqsq qqqqqqqqqq qqqqqqqqqq
     3601 qqqnfnsths ssssghrksy eamiadinkv aeysskigsy sheakvnkwl aeqtamseql
     3661 gaeylnaprr rrprvdpsll dwkkltgeen vavinrltgk kvtgskapql krlgqwlien
     3721 pmfdvdpkwa elvkergnlp hdlqkrlsgn drsnankgks pgrppmmpsp nsqqsanqan
     3781 laatsmasgl nfsslgnlnn sllsglslgn fdpknnpllm pfgglpnlga lsglgnlnnl
     3841 snmsltnslf anlaglglps lagmeaalsa tatstaaetn pvvssvsskn tgstnissas
     3901 kssrnklepp tskslvpsts sasstipttt pfpfffpnps llytplglgg lnpfsmqpgg
     3961 vssayeslaq cgllnpstss astvrkptss asnsrqrdti testtkrkda ekksrysidp
     4021 gitlqqytdm tlqheerrki eqekrenleq ndvadlsdrr terknkeqem kealehlsrt
     4081 saelltrnle dqrsnekrsr nvehsqgseq qtsgmleitl epinkkprid ievsskpvts
     4141 tattpitsad gstgetehaa rssvvtvkqs cieesntfse qssntnkket detvatitti
     4201 stptitvtva statptstpt rtptpistpt stptstptst siitptstts vtigsedaat
     4261 sqsstnnakg dtsklsseve ednkgtkgkk arsgkrlsve ppmerknlrs sagrqaraaa
     4321 erqarmegel qsdsqethtt te
//
LOCUS       XP_034186478            4342 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186478
VERSION     XP_034186478.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330587.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186478.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4342
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X1"
                     /calculated_mol_wt=485264
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2737..2790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2738,2778..2779,2781..2782,2784..2786,2788..2790)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2907..>3176
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3685..3728
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4033..4186
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4342
                     /gene="kis"
                     /coded_by="XM_034330587.2:688..13716"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnsssrddt
     2581 slendiedae sigmmkdskd sdkfsretpt dspaisskad tpgpepsssh egndglldde
     2641 kawpsvvdln trlrrvissy qrsvkkedvk vlqksktgme setsinhnsp mnepplnmqg
     2701 wdlqqlamyl lkmerrekme amvkerertr ieeqktkwsr reeseflrvv stygvnydrk
     2761 kaqydwtkfk siarfdkktd ndltdyymsf ramckkacna klndeddfsd vavdhlspak
     2821 arqildrvdl lskireeils hphleerlsl cqpsgdtpdw wqlgkhdkel llgaakhglg
     2881 rtditilndp dfsfhkllnk nmysniqtsk itdksekaik ienredilkf dkdeilvkle
     2941 kgegtlkiek vgakkdkdin vtdkrtentd seksqvelti ikaenkveek pisnaltitt
     3001 varststdkd hipkvkteek seiviesikn epsktdqimg tnktdevkas nvirsaeetv
     3061 ttikteienq tkekeapkvv ekenqdeksk elqtqdtske tnekssteqk entegtekst
     3121 ennedvtpes dkvnvnavtv keeievqegk ttdtckdkak vckledkcnv qaaelkamfp
     3181 dleviqplsr ltqidtfvlr dkaidynept vvqllshtsn navkwpkeha iearlmhivh
     3241 aiehkewpvs vnfsagdesd ipsnekecse vitittdhgm srnttpgnnv sqskkrkrhi
     3301 aidveterak lhallnsssh tvtqspatls kptvlsgsnt wemndespse esrrstpvqp
     3361 ppahqqartp sipfdlkytv pgkptvipgt sstltpidls agypktstdn nkdimnevqd
     3421 fsmpnknkqi ssnkgkldsm ldklmkrknc ptdepvmgke kkrrkldeiv lglsaakeqq
     3481 sshypehskk stitpnvtvt ptsapmglpn ppsstqkpfs itvtsipssr asnstsglpp
     3541 pslhshkdsf sallvqaeqq nrefkkqqqh qqqsqqqqsq qqqqqqqqqq qqqqqqqqqq
     3601 qqqnfnsths ssssghrksy eamiadinkv aeysskigsy sheakvnkwl aeqtamseql
     3661 gaeylnaprr rrprvdpsll dwkkltgeen vavinrltgk kvtgskapql krlgqwlien
     3721 pmfdvdpkwa elvkergnlp hdlqkrlsgn drsnankgks pgrppmmpsp nsqqsanqan
     3781 laatsmasgl nfsslgnlnn sllsglslgn fdpknnpllm pfgglpnlga lsglgnlnnl
     3841 snmsltnslf anlaglglps lagmeaalsa tatstaaetn pvvssvsskn tgstnissas
     3901 kssrnklepp tskslvpsts sasstipttt pfpfffpnps llytplglgg lnpfsmqpgg
     3961 vssayeslaq cgllnpstss astvrkptss asnsrqrdti testtkrkda ekksrysidp
     4021 gitlqqytdm tlqheerrki eqekrenleq ndvadlsdrr terknkeqem kealehlsrt
     4081 saelltrnle dqrsnekrsr nvehsqgseq qtsgmleitl epinkkprid ievsskpvts
     4141 tattpitsad gstgetehaa rssvvtvkqs cieesntfse qssntnkket detvatitti
     4201 stptitvtva statptstpt rtptpistpt stptstptst siitptstts vtigsedaat
     4261 sqsstnnakg dtsklsseve ednkgtkgkk arsgkrlsve ppmerknlrs sagrqaraaa
     4321 erqarmegel qsdsqethtt te
//
LOCUS       XP_076548218            4342 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X1 [Osmia
            lignaria lignaria].
ACCESSION   XP_076548218
VERSION     XP_076548218.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692103.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4342
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X1"
                     /calculated_mol_wt=485264
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2737..2790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2738,2778..2779,2781..2782,2784..2786,2788..2790)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2907..>3176
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3685..3728
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4033..4186
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4342
                     /gene="kis"
                     /coded_by="XM_076692103.1:462..13490"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnsssrddt
     2581 slendiedae sigmmkdskd sdkfsretpt dspaisskad tpgpepsssh egndglldde
     2641 kawpsvvdln trlrrvissy qrsvkkedvk vlqksktgme setsinhnsp mnepplnmqg
     2701 wdlqqlamyl lkmerrekme amvkerertr ieeqktkwsr reeseflrvv stygvnydrk
     2761 kaqydwtkfk siarfdkktd ndltdyymsf ramckkacna klndeddfsd vavdhlspak
     2821 arqildrvdl lskireeils hphleerlsl cqpsgdtpdw wqlgkhdkel llgaakhglg
     2881 rtditilndp dfsfhkllnk nmysniqtsk itdksekaik ienredilkf dkdeilvkle
     2941 kgegtlkiek vgakkdkdin vtdkrtentd seksqvelti ikaenkveek pisnaltitt
     3001 varststdkd hipkvkteek seiviesikn epsktdqimg tnktdevkas nvirsaeetv
     3061 ttikteienq tkekeapkvv ekenqdeksk elqtqdtske tnekssteqk entegtekst
     3121 ennedvtpes dkvnvnavtv keeievqegk ttdtckdkak vckledkcnv qaaelkamfp
     3181 dleviqplsr ltqidtfvlr dkaidynept vvqllshtsn navkwpkeha iearlmhivh
     3241 aiehkewpvs vnfsagdesd ipsnekecse vitittdhgm srnttpgnnv sqskkrkrhi
     3301 aidveterak lhallnsssh tvtqspatls kptvlsgsnt wemndespse esrrstpvqp
     3361 ppahqqartp sipfdlkytv pgkptvipgt sstltpidls agypktstdn nkdimnevqd
     3421 fsmpnknkqi ssnkgkldsm ldklmkrknc ptdepvmgke kkrrkldeiv lglsaakeqq
     3481 sshypehskk stitpnvtvt ptsapmglpn ppsstqkpfs itvtsipssr asnstsglpp
     3541 pslhshkdsf sallvqaeqq nrefkkqqqh qqqsqqqqsq qqqqqqqqqq qqqqqqqqqq
     3601 qqqnfnsths ssssghrksy eamiadinkv aeysskigsy sheakvnkwl aeqtamseql
     3661 gaeylnaprr rrprvdpsll dwkkltgeen vavinrltgk kvtgskapql krlgqwlien
     3721 pmfdvdpkwa elvkergnlp hdlqkrlsgn drsnankgks pgrppmmpsp nsqqsanqan
     3781 laatsmasgl nfsslgnlnn sllsglslgn fdpknnpllm pfgglpnlga lsglgnlnnl
     3841 snmsltnslf anlaglglps lagmeaalsa tatstaaetn pvvssvsskn tgstnissas
     3901 kssrnklepp tskslvpsts sasstipttt pfpfffpnps llytplglgg lnpfsmqpgg
     3961 vssayeslaq cgllnpstss astvrkptss asnsrqrdti testtkrkda ekksrysidp
     4021 gitlqqytdm tlqheerrki eqekrenleq ndvadlsdrr terknkeqem kealehlsrt
     4081 saelltrnle dqrsnekrsr nvehsqgseq qtsgmleitl epinkkprid ievsskpvts
     4141 tattpitsad gstgetehaa rssvvtvkqs cieesntfse qssntnkket detvatitti
     4201 stptitvtva statptstpt rtptpistpt stptstptst siitptstts vtigsedaat
     4261 sqsstnnakg dtsklsseve ednkgtkgkk arsgkrlsve ppmerknlrs sagrqaraaa
     4321 erqarmegel qsdsqethtt te
//
LOCUS       XP_076548219            4341 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X2 [Osmia
            lignaria lignaria].
ACCESSION   XP_076548219
VERSION     XP_076548219.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692104.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4341
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X2"
                     /calculated_mol_wt=485177
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2736..2789
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2737,2777..2778,2780..2781,2783..2785,2787..2789)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2906..>3175
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3684..3727
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4032..4185
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4341
                     /gene="kis"
                     /coded_by="XM_076692104.1:462..13487"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnssrddts
     2581 lendiedaes igmmkdskds dkfsretptd spaisskadt pgpepssshe gndgllddek
     2641 awpsvvdlnt rlrrvissyq rsvkkedvkv lqksktgmes etsinhnspm nepplnmqgw
     2701 dlqqlamyll kmerrekmea mvkerertri eeqktkwsrr eeseflrvvs tygvnydrkk
     2761 aqydwtkfks iarfdkktdn dltdyymsfr amckkacnak lndeddfsdv avdhlspaka
     2821 rqildrvdll skireeilsh phleerlslc qpsgdtpdww qlgkhdkell lgaakhglgr
     2881 tditilndpd fsfhkllnkn mysniqtski tdksekaiki enredilkfd kdeilvklek
     2941 gegtlkiekv gakkdkdinv tdkrtentds eksqveltii kaenkveekp isnaltittv
     3001 arststdkdh ipkvkteeks eiviesikne psktdqimgt nktdevkasn virsaeetvt
     3061 tikteienqt kekeapkvve kenqdekske lqtqdtsket nekssteqke ntegtekste
     3121 nnedvtpesd kvnvnavtvk eeievqegkt tdtckdkakv ckledkcnvq aaelkamfpd
     3181 leviqplsrl tqidtfvlrd kaidyneptv vqllshtsnn avkwpkehai earlmhivha
     3241 iehkewpvsv nfsagdesdi psnekecsev itittdhgms rnttpgnnvs qskkrkrhia
     3301 idveterakl hallnsssht vtqspatlsk ptvlsgsntw emndespsee srrstpvqpp
     3361 pahqqartps ipfdlkytvp gkptvipgts stltpidlsa gypktstdnn kdimnevqdf
     3421 smpnknkqis snkgkldsml dklmkrkncp tdepvmgkek krrkldeivl glsaakeqqs
     3481 shypehskks titpnvtvtp tsapmglpnp psstqkpfsi tvtsipssra snstsglppp
     3541 slhshkdsfs allvqaeqqn refkkqqqhq qqsqqqqsqq qqqqqqqqqq qqqqqqqqqq
     3601 qqnfnsthss sssghrksye amiadinkva eysskigsys heakvnkwla eqtamseqlg
     3661 aeylnaprrr rprvdpslld wkkltgeenv avinrltgkk vtgskapqlk rlgqwlienp
     3721 mfdvdpkwae lvkergnlph dlqkrlsgnd rsnankgksp grppmmpspn sqqsanqanl
     3781 aatsmasgln fsslgnlnns llsglslgnf dpknnpllmp fgglpnlgal sglgnlnnls
     3841 nmsltnslfa nlaglglpsl agmeaalsat atstaaetnp vvssvssknt gstnissask
     3901 ssrnkleppt skslvpstss asstiptttp fpfffpnpsl lytplglggl npfsmqpggv
     3961 ssayeslaqc gllnpstssa stvrkptssa snsrqrdtit esttkrkdae kksrysidpg
     4021 itlqqytdmt lqheerrkie qekrenleqn dvadlsdrrt erknkeqemk ealehlsrts
     4081 aelltrnled qrsnekrsrn vehsqgseqq tsgmleitle pinkkpridi evsskpvtst
     4141 attpitsadg stgetehaar ssvvtvkqsc ieesntfseq ssntnkketd etvatittis
     4201 tptitvtvas tatptstptr tptpistpts tptstptsts iitptsttsv tigsedaats
     4261 qsstnnakgd tsklssevee dnkgtkgkka rsgkrlsvep pmerknlrss agrqaraaae
     4321 rqarmegelq sdsqethttt e
//
LOCUS       XP_034186481            4340 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X3 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186481
VERSION     XP_034186481.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330590.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186481.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4340
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X3"
                     /calculated_mol_wt=485051
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2735..2788
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2736,2776..2777,2779..2780,2782..2784,2786..2788)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2905..>3174
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3683..3726
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4031..4184
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4340
                     /gene="kis"
                     /coded_by="XM_034330590.2:462..13484"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgkkrrdk fnkktrkfcd eyvpregevv ygswarsecf
     2341 kverglltfg wgrweeilqh sqfrrgwrev dvedcarvil lyclryyrgd ekirnficdl
     2401 itpvengeki knvcvntggr nnrqkkkgrv regretrgsm inggpndpnh wsnaekydgd
     2461 iflesnyrkh lsrhankvll rvrmlyyikh eiigdlvqhi sdgvhvsalr inppqlterp
     2521 akwwdlqadk slivgcykhg yenydqmrid palsfisscp ppsqsqiiqn nsssrddtsl
     2581 endiedaesi gmmkdskdsd kfsretptds paisskadtp gpepsssheg ndgllddeka
     2641 wpsvvdlntr lrrvissyqr svkkedvkvl qksktgmese tsinhnspmn epplnmqgwd
     2701 lqqlamyllk merrekmeam vkerertrie eqktkwsrre eseflrvvst ygvnydrkka
     2761 qydwtkfksi arfdkktdnd ltdyymsfra mckkacnakl ndeddfsdva vdhlspakar
     2821 qildrvdlls kireeilshp hleerlslcq psgdtpdwwq lgkhdkelll gaakhglgrt
     2881 ditilndpdf sfhkllnknm ysniqtskit dksekaikie nredilkfdk deilvklekg
     2941 egtlkiekvg akkdkdinvt dkrtentdse ksqveltiik aenkveekpi snaltittva
     3001 rststdkdhi pkvkteekse iviesiknep sktdqimgtn ktdevkasnv irsaeetvtt
     3061 ikteienqtk ekeapkvvek enqdekskel qtqdtsketn ekssteqken tegteksten
     3121 nedvtpesdk vnvnavtvke eievqegktt dtckdkakvc kledkcnvqa aelkamfpdl
     3181 eviqplsrlt qidtfvlrdk aidyneptvv qllshtsnna vkwpkehaie arlmhivhai
     3241 ehkewpvsvn fsagdesdip snekecsevi tittdhgmsr nttpgnnvsq skkrkrhiai
     3301 dveteraklh allnssshtv tqspatlskp tvlsgsntwe mndespsees rrstpvqppp
     3361 ahqqartpsi pfdlkytvpg kptvipgtss tltpidlsag ypktstdnnk dimnevqdfs
     3421 mpnknkqiss nkgkldsmld klmkrkncpt depvmgkekk rrkldeivlg lsaakeqqss
     3481 hypehskkst itpnvtvtpt sapmglpnpp sstqkpfsit vtsipssras nstsglppps
     3541 lhshkdsfsa llvqaeqqnr efkkqqqhqq qsqqqqsqqq qqqqqqqqqq qqqqqqqqqq
     3601 qnfnsthsss ssghrksyea miadinkvae ysskigsysh eakvnkwlae qtamseqlga
     3661 eylnaprrrr prvdpslldw kkltgeenva vinrltgkkv tgskapqlkr lgqwlienpm
     3721 fdvdpkwael vkergnlphd lqkrlsgndr snankgkspg rppmmpspns qqsanqanla
     3781 atsmasglnf sslgnlnnsl lsglslgnfd pknnpllmpf gglpnlgals glgnlnnlsn
     3841 msltnslfan laglglpsla gmeaalsata tstaaetnpv vssvsskntg stnissasks
     3901 srnkleppts kslvpstssa sstiptttpf pfffpnpsll ytplglggln pfsmqpggvs
     3961 sayeslaqcg llnpstssas tvrkptssas nsrqrdtite sttkrkdaek ksrysidpgi
     4021 tlqqytdmtl qheerrkieq ekrenleqnd vadlsdrrte rknkeqemke alehlsrtsa
     4081 elltrnledq rsnekrsrnv ehsqgseqqt sgmleitlep inkkpridie vsskpvtsta
     4141 ttpitsadgs tgetehaars svvtvkqsci eesntfseqs sntnkketde tvatittist
     4201 ptitvtvast atptstptrt ptpistptst ptstptstsi itptsttsvt igsedaatsq
     4261 sstnnakgdt sklsseveed nkgtkgkkar sgkrlsvepp merknlrssa grqaraaaer
     4321 qarmegelqs dsqethttte
//
LOCUS       XP_034186482            4339 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X4 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186482
VERSION     XP_034186482.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330591.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186482.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4339
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X4"
                     /calculated_mol_wt=484915
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2737..2790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2738,2778..2779,2781..2782,2784..2786,2788..2790)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2904..>3173
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3682..3725
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4030..4183
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4339
                     /gene="kis"
                     /coded_by="XM_034330591.2:462..13481"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnsssrddt
     2581 slendiedae sigmmkdskd sdkfsretpt dspaisskad tpgpepsssh egndglldde
     2641 kawpsvvdln trlrrvissy qrsvkkedvk vlqksktgme setsinhnsp mnepplnmqg
     2701 wdlqqlamyl lkmerrekme amvkerertr ieeqktkwsr reeseflrvv stygvnydrk
     2761 kaqydwtkfk siarfdkktd ndltdyymsf ramckkacna klndeddvav dhlspakarq
     2821 ildrvdllsk ireeilshph leerlslcqp sgdtpdwwql gkhdkelllg aakhglgrtd
     2881 itilndpdfs fhkllnknmy sniqtskitd ksekaikien redilkfdkd eilvklekge
     2941 gtlkiekvga kkdkdinvtd krtentdsek sqveltiika enkveekpis naltittvar
     3001 ststdkdhip kvkteeksei viesikneps ktdqimgtnk tdevkasnvi rsaeetvtti
     3061 kteienqtke keapkvveke nqdekskelq tqdtsketne kssteqkent egtekstenn
     3121 edvtpesdkv nvnavtvkee ievqegkttd tckdkakvck ledkcnvqaa elkamfpdle
     3181 viqplsrltq idtfvlrdka idyneptvvq llshtsnnav kwpkehaiea rlmhivhaie
     3241 hkewpvsvnf sagdesdips nekecsevit ittdhgmsrn ttpgnnvsqs kkrkrhiaid
     3301 veteraklha llnssshtvt qspatlskpt vlsgsntwem ndespseesr rstpvqpppa
     3361 hqqartpsip fdlkytvpgk ptvipgtsst ltpidlsagy pktstdnnkd imnevqdfsm
     3421 pnknkqissn kgkldsmldk lmkrkncptd epvmgkekkr rkldeivlgl saakeqqssh
     3481 ypehskksti tpnvtvtpts apmglpnpps stqkpfsitv tsipssrasn stsglpppsl
     3541 hshkdsfsal lvqaeqqnre fkkqqqhqqq sqqqqsqqqq qqqqqqqqqq qqqqqqqqqq
     3601 nfnsthssss sghrksyeam iadinkvaey sskigsyshe akvnkwlaeq tamseqlgae
     3661 ylnaprrrrp rvdpslldwk kltgeenvav inrltgkkvt gskapqlkrl gqwlienpmf
     3721 dvdpkwaelv kergnlphdl qkrlsgndrs nankgkspgr ppmmpspnsq qsanqanlaa
     3781 tsmasglnfs slgnlnnsll sglslgnfdp knnpllmpfg glpnlgalsg lgnlnnlsnm
     3841 sltnslfanl aglglpslag meaalsatat staaetnpvv ssvsskntgs tnissaskss
     3901 rnklepptsk slvpstssas stiptttpfp fffpnpslly tplglgglnp fsmqpggvss
     3961 ayeslaqcgl lnpstssast vrkptssasn srqrdtites ttkrkdaekk srysidpgit
     4021 lqqytdmtlq heerrkieqe krenleqndv adlsdrrter knkeqemkea lehlsrtsae
     4081 lltrnledqr snekrsrnve hsqgseqqts gmleitlepi nkkpridiev sskpvtstat
     4141 tpitsadgst getehaarss vvtvkqscie esntfseqss ntnkketdet vatittistp
     4201 titvtvasta tptstptrtp tpistptstp tstptstsii tptsttsvti gsedaatsqs
     4261 stnnakgdts klsseveedn kgtkgkkars gkrlsveppm erknlrssag rqaraaaerq
     4321 armegelqsd sqethttte
//
LOCUS       XP_076548220            4338 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X5 [Osmia
            lignaria lignaria].
ACCESSION   XP_076548220
VERSION     XP_076548220.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692105.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4338
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X5"
                     /calculated_mol_wt=484828
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2736..2789
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2737,2777..2778,2780..2781,2783..2785,2787..2789)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2903..>3172
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3681..3724
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <4029..4182
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4338
                     /gene="kis"
                     /coded_by="XM_076692105.1:462..13478"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnssrddts
     2581 lendiedaes igmmkdskds dkfsretptd spaisskadt pgpepssshe gndgllddek
     2641 awpsvvdlnt rlrrvissyq rsvkkedvkv lqksktgmes etsinhnspm nepplnmqgw
     2701 dlqqlamyll kmerrekmea mvkerertri eeqktkwsrr eeseflrvvs tygvnydrkk
     2761 aqydwtkfks iarfdkktdn dltdyymsfr amckkacnak lndeddvavd hlspakarqi
     2821 ldrvdllski reeilshphl eerlslcqps gdtpdwwqlg khdkelllga akhglgrtdi
     2881 tilndpdfsf hkllnknmys niqtskitdk sekaikienr edilkfdkde ilvklekgeg
     2941 tlkiekvgak kdkdinvtdk rtentdseks qveltiikae nkveekpisn altittvars
     3001 tstdkdhipk vkteekseiv iesiknepsk tdqimgtnkt devkasnvir saeetvttik
     3061 teienqtkek eapkvveken qdekskelqt qdtsketnek ssteqkente gtekstenne
     3121 dvtpesdkvn vnavtvkeei evqegkttdt ckdkakvckl edkcnvqaae lkamfpdlev
     3181 iqplsrltqi dtfvlrdkai dyneptvvql lshtsnnavk wpkehaiear lmhivhaieh
     3241 kewpvsvnfs agdesdipsn ekecseviti ttdhgmsrnt tpgnnvsqsk krkrhiaidv
     3301 eteraklhal lnssshtvtq spatlskptv lsgsntwemn despseesrr stpvqpppah
     3361 qqartpsipf dlkytvpgkp tvipgtsstl tpidlsagyp ktstdnnkdi mnevqdfsmp
     3421 nknkqissnk gkldsmldkl mkrkncptde pvmgkekkrr kldeivlgls aakeqqsshy
     3481 pehskkstit pnvtvtptsa pmglpnppss tqkpfsitvt sipssrasns tsglpppslh
     3541 shkdsfsall vqaeqqnref kkqqqhqqqs qqqqsqqqqq qqqqqqqqqq qqqqqqqqqn
     3601 fnsthsssss ghrksyeami adinkvaeys skigsyshea kvnkwlaeqt amseqlgaey
     3661 lnaprrrrpr vdpslldwkk ltgeenvavi nrltgkkvtg skapqlkrlg qwlienpmfd
     3721 vdpkwaelvk ergnlphdlq krlsgndrsn ankgkspgrp pmmpspnsqq sanqanlaat
     3781 smasglnfss lgnlnnslls glslgnfdpk nnpllmpfgg lpnlgalsgl gnlnnlsnms
     3841 ltnslfanla glglpslagm eaalsatats taaetnpvvs svsskntgst nissaskssr
     3901 nklepptsks lvpstssass tiptttpfpf ffpnpsllyt plglgglnpf smqpggvssa
     3961 yeslaqcgll npstssastv rkptssasns rqrdtitest tkrkdaekks rysidpgitl
     4021 qqytdmtlqh eerrkieqek renleqndva dlsdrrterk nkeqemkeal ehlsrtsael
     4081 ltrnledqrs nekrsrnveh sqgseqqtsg mleitlepin kkpridievs skpvtstatt
     4141 pitsadgstg etehaarssv vtvkqsciee sntfseqssn tnkketdetv atittistpt
     4201 itvtvastat ptstptrtpt pistptstpt stptstsiit ptsttsvtig sedaatsqss
     4261 tnnakgdtsk lsseveednk gtkgkkarsg krlsveppme rknlrssagr qaraaaerqa
     4321 rmegelqsds qethttte
//
LOCUS       XP_034186483            4278 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X6 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186483
VERSION     XP_034186483.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330592.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186483.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4278
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X6"
                     /calculated_mol_wt=477938
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2673..2726
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2674,2714..2715,2717..2718,2720..2722,2724..2726)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2843..>3112
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3621..3664
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <3969..4122
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4278
                     /gene="kis"
                     /coded_by="XM_034330592.2:462..13298"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvss ssrddtslen
     2521 diedaesigm mkdskdsdkf sretptdspa isskadtpgp epssshegnd gllddekawp
     2581 svvdlntrlr rvissyqrsv kkedvkvlqk sktgmesets inhnspmnep plnmqgwdlq
     2641 qlamyllkme rrekmeamvk erertrieeq ktkwsrrees eflrvvstyg vnydrkkaqy
     2701 dwtkfksiar fdkktdndlt dyymsframc kkacnaklnd eddfsdvavd hlspakarqi
     2761 ldrvdllski reeilshphl eerlslcqps gdtpdwwqlg khdkelllga akhglgrtdi
     2821 tilndpdfsf hkllnknmys niqtskitdk sekaikienr edilkfdkde ilvklekgeg
     2881 tlkiekvgak kdkdinvtdk rtentdseks qveltiikae nkveekpisn altittvars
     2941 tstdkdhipk vkteekseiv iesiknepsk tdqimgtnkt devkasnvir saeetvttik
     3001 teienqtkek eapkvveken qdekskelqt qdtsketnek ssteqkente gtekstenne
     3061 dvtpesdkvn vnavtvkeei evqegkttdt ckdkakvckl edkcnvqaae lkamfpdlev
     3121 iqplsrltqi dtfvlrdkai dyneptvvql lshtsnnavk wpkehaiear lmhivhaieh
     3181 kewpvsvnfs agdesdipsn ekecseviti ttdhgmsrnt tpgnnvsqsk krkrhiaidv
     3241 eteraklhal lnssshtvtq spatlskptv lsgsntwemn despseesrr stpvqpppah
     3301 qqartpsipf dlkytvpgkp tvipgtsstl tpidlsagyp ktstdnnkdi mnevqdfsmp
     3361 nknkqissnk gkldsmldkl mkrkncptde pvmgkekkrr kldeivlgls aakeqqsshy
     3421 pehskkstit pnvtvtptsa pmglpnppss tqkpfsitvt sipssrasns tsglpppslh
     3481 shkdsfsall vqaeqqnref kkqqqhqqqs qqqqsqqqqq qqqqqqqqqq qqqqqqqqqn
     3541 fnsthsssss ghrksyeami adinkvaeys skigsyshea kvnkwlaeqt amseqlgaey
     3601 lnaprrrrpr vdpslldwkk ltgeenvavi nrltgkkvtg skapqlkrlg qwlienpmfd
     3661 vdpkwaelvk ergnlphdlq krlsgndrsn ankgkspgrp pmmpspnsqq sanqanlaat
     3721 smasglnfss lgnlnnslls glslgnfdpk nnpllmpfgg lpnlgalsgl gnlnnlsnms
     3781 ltnslfanla glglpslagm eaalsatats taaetnpvvs svsskntgst nissaskssr
     3841 nklepptsks lvpstssass tiptttpfpf ffpnpsllyt plglgglnpf smqpggvssa
     3901 yeslaqcgll npstssastv rkptssasns rqrdtitest tkrkdaekks rysidpgitl
     3961 qqytdmtlqh eerrkieqek renleqndva dlsdrrterk nkeqemkeal ehlsrtsael
     4021 ltrnledqrs nekrsrnveh sqgseqqtsg mleitlepin kkpridievs skpvtstatt
     4081 pitsadgstg etehaarssv vtvkqsciee sntfseqssn tnkketdetv atittistpt
     4141 itvtvastat ptstptrtpt pistptstpt stptstsiit ptsttsvtig sedaatsqss
     4201 tnnakgdtsk lsseveednk gtkgkkarsg krlsveppme rknlrssagr qaraaaerqa
     4261 rmegelqsds qethttte
//
LOCUS       XP_034186484            4277 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X7 [Osmia
            lignaria lignaria].
ACCESSION   XP_034186484
VERSION     XP_034186484.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330593.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186484.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4277
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X7"
                     /calculated_mol_wt=477851
     Region          <102..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2672..2725
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2673,2713..2714,2716..2717,2719..2721,2723..2725)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2842..>3111
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3620..3663
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <3968..4121
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4277
                     /gene="kis"
                     /coded_by="XM_034330593.2:462..13295"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvss srddtslend
     2521 iedaesigmm kdskdsdkfs retptdspai sskadtpgpe pssshegndg llddekawps
     2581 vvdlntrlrr vissyqrsvk kedvkvlqks ktgmesetsi nhnspmnepp lnmqgwdlqq
     2641 lamyllkmer rekmeamvke rertrieeqk tkwsrreese flrvvstygv nydrkkaqyd
     2701 wtkfksiarf dkktdndltd yymsframck kacnaklnde ddfsdvavdh lspakarqil
     2761 drvdllskir eeilshphle erlslcqpsg dtpdwwqlgk hdkelllgaa khglgrtdit
     2821 ilndpdfsfh kllnknmysn iqtskitdks ekaikienre dilkfdkdei lvklekgegt
     2881 lkiekvgakk dkdinvtdkr tentdseksq veltiikaen kveekpisna ltittvarst
     2941 stdkdhipkv kteekseivi esiknepskt dqimgtnktd evkasnvirs aeetvttikt
     3001 eienqtkeke apkvvekenq dekskelqtq dtsketneks steqkenteg tekstenned
     3061 vtpesdkvnv navtvkeeie vqegkttdtc kdkakvckle dkcnvqaael kamfpdlevi
     3121 qplsrltqid tfvlrdkaid yneptvvqll shtsnnavkw pkehaiearl mhivhaiehk
     3181 ewpvsvnfsa gdesdipsne kecsevitit tdhgmsrntt pgnnvsqskk rkrhiaidve
     3241 teraklhall nssshtvtqs patlskptvl sgsntwemnd espseesrrs tpvqpppahq
     3301 qartpsipfd lkytvpgkpt vipgtsstlt pidlsagypk tstdnnkdim nevqdfsmpn
     3361 knkqissnkg kldsmldklm krkncptdep vmgkekkrrk ldeivlglsa akeqqsshyp
     3421 ehskkstitp nvtvtptsap mglpnppsst qkpfsitvts ipssrasnst sglpppslhs
     3481 hkdsfsallv qaeqqnrefk kqqqhqqqsq qqqsqqqqqq qqqqqqqqqq qqqqqqqqnf
     3541 nsthsssssg hrksyeamia dinkvaeyss kigsysheak vnkwlaeqta mseqlgaeyl
     3601 naprrrrprv dpslldwkkl tgeenvavin rltgkkvtgs kapqlkrlgq wlienpmfdv
     3661 dpkwaelvke rgnlphdlqk rlsgndrsna nkgkspgrpp mmpspnsqqs anqanlaats
     3721 masglnfssl gnlnnsllsg lslgnfdpkn npllmpfggl pnlgalsglg nlnnlsnmsl
     3781 tnslfanlag lglpslagme aalsatatst aaetnpvvss vsskntgstn issaskssrn
     3841 klepptsksl vpstssasst iptttpfpff fpnpsllytp lglgglnpfs mqpggvssay
     3901 eslaqcglln pstssastvr kptssasnsr qrdtitestt krkdaekksr ysidpgitlq
     3961 qytdmtlqhe errkieqekr enleqndvad lsdrrterkn keqemkeale hlsrtsaell
     4021 trnledqrsn ekrsrnvehs qgseqqtsgm leitlepink kpridievss kpvtstattp
     4081 itsadgstge tehaarssvv tvkqsciees ntfseqssnt nkketdetva tittistpti
     4141 tvtvastatp tstptrtptp istptstpts tptstsiitp tsttsvtigs edaatsqsst
     4201 nnakgdtskl sseveednkg tkgkkarsgk rlsveppmer knlrssagrq araaaerqar
     4261 megelqsdsq ethttte
//
LOCUS       XP_076548221            4275 aa            linear   INV 03-SEP-2025
DEFINITION  chromodomain helicase DNA binding protein kismet isoform X8 [Osmia
            lignaria lignaria].
ACCESSION   XP_076548221
VERSION     XP_076548221.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692106.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..4275
                     /product="chromodomain helicase DNA binding protein kismet
                     isoform X8"
                     /calculated_mol_wt=478453
     Region          64..>472
                     /region_name="Med15"
                     /note="ARC105 or Med15 subunit of Mediator complex
                     non-fungal; pfam09606"
                     /db_xref="CDD:312941"
     Region          <307..>607
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <308..>450
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          <845..>1009
                     /region_name="PAT1"
                     /note="Topoisomerase II-associated protein PAT1;
                     pfam09770"
                     /db_xref="CDD:401645"
     Region          1509..1569
                     /region_name="CD1_tandem_CHD5-9_like"
                     /note="repeat 1 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18668"
                     /db_xref="CDD:349315"
     Site            order(1537,1539..1540,1544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:349315"
     Region          1588..1646
                     /region_name="CD2_tandem_CHD5-9_like"
                     /note="repeat 2 of the paired tandem chromodomains of
                     chromodomain helicase DNA-binding protein 5-9, and similar
                     proteins; cd18663"
                     /db_xref="CDD:349310"
     Site            order(1613,1630)
                     /site_type="other"
                     /note="putative peptide binding site [polypeptide
                     binding]"
                     /db_xref="CDD:349310"
     Region          1680..>2205
                     /region_name="PLN03142"
                     /note="Probable chromatin-remodeling complex ATPase chain;
                     Provisional"
                     /db_xref="CDD:215601"
     Region          2737..2790
                     /region_name="SANT"
                     /note="'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains.
                     Tandem copies of the domain bind telomeric DNA tandem
                     repeatsas part of the capping complex. Binding is sequence
                     dependent for repeats which contain the G/C rich motif
                     [C2-3 A (CA)1-6]. The domain is...; cd00167"
                     /db_xref="CDD:238096"
     Site            order(2738,2778..2779,2781..2782,2784..2786,2788..2790)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:238096"
     Region          <2907..>3176
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          3618..3661
                     /region_name="BRK"
                     /note="BRK domain; pfam07533"
                     /db_xref="CDD:462196"
     Region          <3966..4119
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..4275
                     /gene="kis"
                     /coded_by="XM_076692106.1:462..13289"
                     /db_xref="GeneID:117607200"
ORIGIN      
        1 mdsydlfged vsgsmldalp dlggndhfdp agttnsvvvs rdgpnpgvng ggsyisqsyi
       61 sqesslqklt sfggpefgss nqlqnpsqrn mfnqnlgtfd sgapqraivp gafqnqarnm
      121 apqhrgphpg sggqypnynd nmysmeqqhs mtranmsmdp smqgwpgsgs aypqmqrhyn
      181 qmapqpgtyr qhippqyshq qdqggvmnqk lqqqqhfgnq qsmigtmgvp hqamqtggtg
      241 svyqhmvgqq qhyqqgnsvt myqtspgypg fasaihqqqq qqqqqqnqqp pqrmphhhps
      301 hpthqthpsh tqqypqhtqh pnpqqqpidp qyshhassmf nqpqhsgpat qfgtakhats
      361 pqyratfpql spqmsprpqm sprppavqqq msprpimspa kpntlsphph vqlphqqsnq
      421 patmsvspcp pasmqmnpss qqttlqaleq mvipgvaasn snvsnqdynq yqarpamsqt
      481 pnilsqstaq nsiqpitgpr mpmpaqwshq qqqqqqqqqq qqqqqqsqir pntsedrpvv
      541 eaqppvseqs tpmfpvtgte tvhsvaettt rdstdsvhnp ieppkqesev lhqnvqepqq
      601 dvlnqptdpl mqnnsqnnsp lehtagkisq etvqslpass stngnvdcps lpalpqqqit
      661 qsqiqtpvst venqskcdtk qpdqhleina vscapassni tahsqqpavs sqatqqqqqq
      721 hnqqqpincp qqqvqqihln qpqvvsmqqs pvqsqqplqp sqqpqlsqvq qlgqqsqqvq
      781 qinttqqqap iaqspqstln qpqqsqpqis qsqlaqpsqp sqstpvcisq qvpqqstqqs
      841 qplvtsqvgi vpqnaplipp qiphqpqlqa piqnttpqlp sqtnqvppth lngqqiptvn
      901 qsssivlpsh qtavsvpnsq tshpvqqnft eytnipknni fpnemmvgsg qqypssgdmi
      961 sgntipnmyn mppnptstgv tsggfstsct pvthhqeraa lqqqlqelyc mppatenqek
     1021 ivaiqeklqa lqqhesndqc nggpqcilqt pmfttavvds pqvssttgrg rskgtprakk
     1081 srkkadkeav appivttqqq peqplsdnqv tpgdssnivd saadsedikp ssgdiedewn
     1141 kgrksrpprk prkpkepkep kepkepkvkk epkppkeaks pkaprkrnkd kdstkrnrkk
     1201 tnqsesadde iaqeteetav sdssaikead vklsepsiqg dqeqvdssts eplivetkdq
     1261 skqkteseds ptdekkedns dagtnietat pnkkkaaark rsvgkvsagk sprsskkrks
     1321 riapdsdgee laatpppspe agddnkrrsa rnthrkkyvd dvmlrfsddd vplqdaqlsk
     1381 kvdgvnttkp vsvkkdnegg evgpnkpnfv yinttdedsm vvqhilcsri gkreikptip
     1441 evkaesseek nentvsdtkg eekedelkee vsekelvsea tnkdekdaei sedkdtddnd
     1501 vkdkskheea vteskqesav vdvkpqivev eeyfvkyrnf sylhcewrte eelykgdkri
     1561 qaklkrfkqr qqqntnifen teddpfnpdf vevdrvldea thtdpttget lkhylvkwrs
     1621 lqyedstwel eedvdpekia qfikfnkvpp kdqwkpkkkp nasawvklee spiyknnnsl
     1681 rpyqleglnw llfswynghn cilademglg ktiqsltfvd avykygirgp fliiaplsti
     1741 pnwqrefesw tdmnvvvyhg saasrtmlqe yevyyknekg qqikdlikfn vlittfeiii
     1801 tdfnelrgyn wrlcvideah rlknrnckll eglrqlnleh rvllsgtplq nnvnelfsll
     1861 nflepvqfss seaflkefgn lssesevhkl qlllkpmmlr rlkedveksl apkeetvvev
     1921 eltniqkkyy rgilernfsf lakgttsani pnlmntmmel rkccihpfll ngaedqiqld
     1981 yktgekedse ayyhalvnss gkmvlidkll pklkasghrv lifsqmvkcl dlledyllyk
     2041 kypyeridgr irgnlrqaai dryskpdsdr fvfllctkag glginltaad tviiydsdwn
     2101 pqndlqaqar chrigqqkmv kvyrllcrnt yeremfdkas lklgldkail qsmntsqggk
     2161 dpsnkqltkk eiedllkkga ygaimdddna gdkfceedie qilerrtqii tieaekgstf
     2221 skasfacssn rsdiniddpd fwkkwakkae idttekkeed elviseprrr tqikryghde
     2281 svvmsdldss ddnsddetss gltgrgkkrr dkfnkktrkf cdeyvprege vvygswarse
     2341 cfkvergllt fgwgrweeil qhsqfrrgwr evdvedcarv illyclryyr gdekirnfic
     2401 dlitpvenge kiknvcvntg grnnrqkkkg rvregretrg sminggpndp nhwsnaekyd
     2461 gdiflesnyr khlsrhankv llrvrmlyyi kheiigdlvq hisdgvhvsa lrinppqlte
     2521 rpakwwdlqa dkslivgcyk hgyenydqmr idpalsfiss cpppsqsqii qnnsssrddt
     2581 slendiedae sigmmkdskd sdkfsretpt dspaisskad tpgpepsssh egndglldde
     2641 kawpsvvdln trlrrvissy qrsvkkedvk vlqksktgme setsinhnsp mnepplnmqg
     2701 wdlqqlamyl lkmerrekme amvkerertr ieeqktkwsr reeseflrvv stygvnydrk
     2761 kaqydwtkfk siarfdkktd ndltdyymsf ramckkacna klndeddfsd vavdhlspak
     2821 arqildrvdl lskireeils hphleerlsl cqpsgdtpdw wqlgkhdkel llgaakhglg
     2881 rtditilndp dfsfhkllnk nmysniqtsk itdksekaik ienredilkf dkdeilvkle
     2941 kgegtlkiek vgakkdkdin vtdkrtentd seksqvelti ikaenkveek pisnaltitt
     3001 varststdkd hipkvkteek seiviesikn epsktdqimg tnktdevkas nvirsaeetv
     3061 ttikteienq tkekeapkvv ekenqdeksk elqtqdtske tnekssteqk entegtekst
     3121 ennedvtpes dkvnvnavtv keeievqegk ttdtckdkak vckledkcnv qaaelkamfp
     3181 dleviqplsr ltqidtfvlr dkaidynept vvqllshtsn navkwpkeha iearlmhivh
     3241 aiehkewpvs vnfsagdesd ipsnekecse vitittdhgm srnttpgnnv sqskkrkrhi
     3301 aidveterak lhallnsssh tvtqspatls kptvlsgsnt wemndespse esrrstpvqp
     3361 ppahqqartp sipfdlkytv pgkptvipgt sstltpidls agypktstdn nkdimnevqd
     3421 fsmpnknkqi ssnkgkldsm ldklmkrknc ptdepvmgke kkrrkldeiv lglsaakeqq
     3481 sshypehskk kqqnrefkkq qqhqqqsqqq qsqqqqqqqq qqqqqqqqqq qqqqqqnfns
     3541 thsssssghr ksyeamiadi nkvaeysski gsysheakvn kwlaeqtams eqlgaeylna
     3601 prrrrprvdp slldwkkltg eenvavinrl tgkkvtgska pqlkrlgqwl ienpmfdvdp
     3661 kwaelvkerg nlphdlqkrl sgndrsnank gkspgrppmm pspnsqqsan qanlaatsma
     3721 sglnfsslgn lnnsllsgls lgnfdpknnp llmpfgglpn lgalsglgnl nnlsnmsltn
     3781 slfanlaglg lpslagmeaa lsatatstaa etnpvvssvs skntgstnis saskssrnkl
     3841 epptskslvp stssasstip tttpfpfffp npsllytplg lgglnpfsmq pggvssayes
     3901 laqcgllnps tssastvrkp tssasnsrqr dtitesttkr kdaekksrys idpgitlqqy
     3961 tdmtlqheer rkieqekren leqndvadls drrterknke qemkealehl srtsaelltr
     4021 nledqrsnek rsrnvehsqg seqqtsgmle itlepinkkp ridievsskp vtstattpit
     4081 sadgstgete haarssvvtv kqscieesnt fseqssntnk ketdetvati ttistptitv
     4141 tvastatpts tptrtptpis tptstptstp tstsiitpts ttsvtigsed aatsqsstnn
     4201 akgdtsklss eveednkgtk gkkarsgkrl sveppmerkn lrssagrqar aaaerqarme
     4261 gelqsdsqet httte
//
LOCUS       XP_034186486            1976 aa            linear   INV 03-SEP-2025
DEFINITION  nucleolar pre-ribosomal-associated protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186486
VERSION     XP_034186486.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330595.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186486.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1976
                     /product="nucleolar pre-ribosomal-associated protein 1"
                     /calculated_mol_wt=227476
     Region          170..487
                     /region_name="Npa1"
                     /note="Ribosome 60S biogenesis N-terminal; pfam11707"
                     /db_xref="CDD:463325"
     Region          1632..1826
                     /region_name="NopRA1"
                     /note="Nucleolar pre-ribosomal-associated protein 1;
                     pfam16201"
                     /db_xref="CDD:465063"
     CDS             1..1976
                     /gene="LOC117607201"
                     /coded_by="XM_034330595.2:179..6109"
                     /db_xref="GeneID:117607201"
ORIGIN      
        1 mrisskneks qnhsavkknk kekdikgdid inehvkaidk kklkrnwssd enevrkkhkt
       61 kdnlnplvng sndngyttef dketfiesqg skrtkhlkst tecnnknekt venkentneq
      121 teylsgkllr kslstinnve mlkmfikiyk eneerdlaae ylnaggnile vlriidttdr
      181 knignvvtvf stirilliki laqypqhqps aqeacrhlin shlslihsml svqsnakhrk
      241 vilqllaavv slggnlpcel lvhlsippev ikslvqhtkp sdkqnirncf ihfiiaflie
      301 gnvqiikall ekrgllssif pdliydskdi vnlvittlkn yilknsnvtk tmklhifsts
      361 vvqnivclyn wkgpsnwpkn knqdftshpq ylaekevvte ivhdfliill tshkygiifh
      421 drmlgtshvk hnnlvntviq sldrpweyek psdlvvkima acpdlikfqf silepylepr
      481 vsvkwiavmq firkiinsld meacvkicss elnvlqlanv imsltlhgvv lkhaiipslt
      541 hsnitirhea iltlliifnq ikqyllitka fyndsyfctf knciiesiik hvpilnmtlk
      601 vwisvsvsds vesentdkeh vpktnkhehl tavlnllhvy nevcpklldt ladiesvtfl
      661 nilnelngid inefniikvk aiqflviltp neflpqqkif kdtlsflipl lnekaslvss
      721 saeatiktll nttgmfegcn dhldiwvkgf tnlsdkkeat kwfihnikka asniekyane
      781 iikieeside gaiyggried ivrelenkdn iyesldnivl rmqrftsisp llccmlhkrr
      841 kdlhptiisy lsyvlihtmh yqvapqcliy fvkdielpvk eyllswlegn dpiyikevfp
      901 smnltcklns vllsndklqi neifgggkst vtfqyndeei tihhslsgyd ithlfkmilf
      961 ylvqftkrgs ltkiqtdnck illisllyva kdnltdfklv ekcatfifth piilhyfspf
     1021 hhkskdsiks mitliiadvs kavirlckey darnlflhfk nklimllckm idkrkkrdki
     1081 nnvktittvl vslqltpqnv vhllkklinl esamfiande knlslygyiv pelleiinsn
     1141 emqsersalf dldaefvkhl ylhllflksh lvnnlemwei slykylskfp fnivdidanm
     1201 ftallstkmt dttvklisfl tckntkfmpi ftrymmksen ikkgsivfpi igsnlnfewn
     1261 hnflenlkqy yeteilsylc npkntkiwie envaaisyli thafdftacn ktcksilqig
     1321 dkldtvsihy lkilqsmydk caaseiddeq fimswtqvll hiisltlkrd sknvkkiyil
     1381 cellndavky ltnkkkdfif eelstnnsws qftrfslkls lkglkdngqh vpllktlstl
     1441 cnvayrnnsd deyartlfem atshsafini mlessdikre ltellwiliq khkkimtlth
     1501 ipvylaayna tlseadqyil filqhyeand iniyeyrpyv wgsvaavhys vkgephmnlw
     1561 rqpsitqvln lfeedkvnnt iknypvnral knselyeann tydptfylpl lhfllsennv
     1621 vscqrfaqcg alaltfaacs svhsdvrmla ytiiaryynh leassskakl lwmrlidalr
     1681 ygivslqsel snvrlnsivs tflartslva tkplhplyqt lqtfllakpa lnvntipell
     1741 qlfhssdvkh kshrcwilen irdgmrtese ldvafkcnlf kmlldfyvcn lsdsdtkili
     1801 leiinvtlki tkasillieg hgllpwllgi ttnlckqear hfelimeiin kllstilnik
     1861 ansnqfkmml lnivlnlksh lskdikistf ilyinilqkl ietksikmiv tekdimeilg
     1921 fskellgdid ecdemlrfgy kyitktncss qnetevarns lktlmwtwcs ndicil
//
LOCUS       XP_076548222             684 aa            linear   INV 03-SEP-2025
DEFINITION  F box and leucine-rich-repeat gene 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548222
VERSION     XP_076548222.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692107.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="F box and leucine-rich-repeat gene 4 isoform X1"
                     /calculated_mol_wt=78793
     Region          339..382
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          406..435
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <431..573
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          436..461
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          462..486
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          487..511
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          512..539
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          540..564
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          565..592
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          644..661
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..684
                     /gene="Fbxl4"
                     /coded_by="XM_076692107.1:397..2451"
                     /db_xref="GeneID:117607207"
ORIGIN      
        1 mishdeikie vvpisgkrid ekeetvtfve qfvkdvcdfs sqygsnisis ytayniagnp
       61 skfpdygdfp qafvmrtygq wwdkapsrlt dympqnnvdv vsqdyidley yqevypiris
      121 vyetynpgsv vgiwaqnseg kwfqlwsgfp qvvphkprif spylqscnfk tkmirlefnh
      181 nlldyyteld avlligtsel ivpnnlyhqn lndlsqelgy lkqsnddiyn ltpdylkanq
      241 dlavikkilp khcrllkski idniskgkli skigqhyqsv ppieeafnsl qqflqedfpk
      301 likdinhsis ntlneqsnst kerfllplnd sdnqpcgsfs tlpdetvlki lknldlkslc
      361 rlcrvnrhfn niardallyt slnlkpywhc ldtsalncla lrchylqrld lswcgnynmi
      421 kykdftdflh tsgtllthlr lnccqfvnde vifeiskvck nlkelclrnc mgvtnegfsn
      481 lenlkllerl elyrtsvets tlcsilkknt qmrhlnlagm hdrlnideia ielgnscpyl
      541 esvdfwkaqt ltphgvkals hctnlrevdf gwcggmgapg dslrallfsc rylekvflaa
      601 lrgltdrdle plllcqrlqq ldllgarslt peicygfllf cprlemidls fcegindfii
      661 qewrqqyprv sikrsfqvts sdml
//
LOCUS       XP_034186512             684 aa            linear   INV 03-SEP-2025
DEFINITION  F box and leucine-rich-repeat gene 4 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186512
VERSION     XP_034186512.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330621.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..684
                     /product="F box and leucine-rich-repeat gene 4 isoform X1"
                     /calculated_mol_wt=78793
     Region          339..382
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          406..435
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <431..573
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          436..461
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          462..486
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          487..511
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          512..539
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          540..564
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          565..592
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          644..661
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..684
                     /gene="Fbxl4"
                     /coded_by="XM_034330621.2:256..2310"
                     /db_xref="GeneID:117607207"
ORIGIN      
        1 mishdeikie vvpisgkrid ekeetvtfve qfvkdvcdfs sqygsnisis ytayniagnp
       61 skfpdygdfp qafvmrtygq wwdkapsrlt dympqnnvdv vsqdyidley yqevypiris
      121 vyetynpgsv vgiwaqnseg kwfqlwsgfp qvvphkprif spylqscnfk tkmirlefnh
      181 nlldyyteld avlligtsel ivpnnlyhqn lndlsqelgy lkqsnddiyn ltpdylkanq
      241 dlavikkilp khcrllkski idniskgkli skigqhyqsv ppieeafnsl qqflqedfpk
      301 likdinhsis ntlneqsnst kerfllplnd sdnqpcgsfs tlpdetvlki lknldlkslc
      361 rlcrvnrhfn niardallyt slnlkpywhc ldtsalncla lrchylqrld lswcgnynmi
      421 kykdftdflh tsgtllthlr lnccqfvnde vifeiskvck nlkelclrnc mgvtnegfsn
      481 lenlkllerl elyrtsvets tlcsilkknt qmrhlnlagm hdrlnideia ielgnscpyl
      541 esvdfwkaqt ltphgvkals hctnlrevdf gwcggmgapg dslrallfsc rylekvflaa
      601 lrgltdrdle plllcqrlqq ldllgarslt peicygfllf cprlemidls fcegindfii
      661 qewrqqyprv sikrsfqvts sdml
//
LOCUS       XP_034186513             661 aa            linear   INV 03-SEP-2025
DEFINITION  F box and leucine-rich-repeat gene 4 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186513
VERSION     XP_034186513.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330622.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..661
                     /product="F box and leucine-rich-repeat gene 4 isoform X2"
                     /calculated_mol_wt=75952
     Region          316..359
                     /region_name="F-box-like"
                     /note="pfam12937"
                     /db_xref="CDD:463757"
     Region          383..412
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          <408..550
                     /region_name="AMN1"
                     /note="Antagonist of mitotic exit network protein 1;
                     cd09293"
                     /db_xref="CDD:187754"
     Region          413..438
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          439..463
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          464..488
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          489..516
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          517..541
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          542..569
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     Region          621..638
                     /region_name="leucine-rich repeat"
                     /note="leucine-rich repeat [structural motif]"
                     /db_xref="CDD:275381"
     CDS             1..661
                     /gene="Fbxl4"
                     /coded_by="XM_034330622.2:398..2383"
                     /db_xref="GeneID:117607207"
ORIGIN      
        1 mishdeikie vvpisgkrid ekeetvtfve qfvkdvcdfs sqygsnisis ytayniagnp
       61 skfpdygdfp qafvmrtygq wwdkapsrlt dympqnnvdv vsqdyidley yqevypiris
      121 vyetynpgsv vgiwaqnseg kwfqlwsgfp qvvphkprif spylqsyavl ligtselivp
      181 nnlyhqnlnd lsqelgylkq snddiynltp dylkanqdla vikkilpkhc rllkskiidn
      241 iskgkliski gqhyqsvppi eeafnslqqf lqedfpklik dinhsisntl neqsnstker
      301 fllplndsdn qpcgsfstlp detvlkilkn ldlkslcrlc rvnrhfnnia rdallytsln
      361 lkpywhcldt salnclalrc hylqrldlsw cgnynmikyk dftdflhtsg tllthlrlnc
      421 cqfvndevif eiskvcknlk elclrncmgv tnegfsnlen lkllerlely rtsvetstlc
      481 silkkntqmr hlnlagmhdr lnideiaiel gnscpylesv dfwkaqtltp hgvkalshct
      541 nlrevdfgwc ggmgapgdsl rallfscryl ekvflaalrg ltdrdlepll lcqrlqqldl
      601 lgarsltpei cygfllfcpr lemidlsfce gindfiiqew rqqyprvsik rsfqvtssdm
      661 l
//
LOCUS       XP_034186514             657 aa            linear   INV 03-SEP-2025
DEFINITION  brf RNA polymerase III subunit isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186514
VERSION     XP_034186514.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330623.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..657
                     /product="brf RNA polymerase III subunit isoform X1"
                     /calculated_mol_wt=74275
     Region          5..40
                     /region_name="TF_Zn_Ribbon"
                     /note="TFIIB zinc-binding; pfam08271"
                     /db_xref="CDD:400527"
     Region          83..173
                     /region_name="CYCLIN_TFIIIB90_rpt1"
                     /note="first cyclin box found in transcription factor IIIB
                     90 kDa subunit (TFIIIB90) and similar proteins; cd20553"
                     /db_xref="CDD:410256"
     Site            order(84,123..126,160,164,167..168)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410256"
     Region          178..269
                     /region_name="CYCLIN_TFIIIB90_rpt2"
                     /note="second cyclin box found in transcription factor
                     IIIB 90 kDa subunit (TFIIIB90) and similar proteins;
                     cd20554"
                     /db_xref="CDD:410257"
     Site            order(221..223,254..255,257..258)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410257"
     Region          427..523
                     /region_name="BRF1"
                     /note="Brf1-like TBP-binding domain; pfam07741"
                     /db_xref="CDD:462250"
     CDS             1..657
                     /gene="Brf"
                     /coded_by="XM_034330623.2:303..2276"
                     /db_xref="GeneID:117607209"
ORIGIN      
        1 msgskcrncg steietdpar gdavctecgf vledqlivse tafketpsgn mmvlgqfvan
       61 dstggatefg atyhingkes rgitlqnark githlcmqlr lnqhcidtsm nfykmalnrh
      121 ltrgrkqahn haacvyitcr tegtahmlid isdvlqicvh elgrtylrft qalcinipsv
      181 dpclyilrfa nklefgekth evsmtalrvv qrmkrdsihs grrpsglcga allmaarlhe
      241 fnrspadiik ivkvhestlr krliefgdtp ssaltleefm tvdleeeqdp pafkaarkkd
      301 rerlqkleni yteinelqae idkqleehsv gktrkrkdav cleredterf vresnlnvik
      361 syvedaddpd seiqesnlnn tnnrlitglg pniasmglis andrenetng qtnatyendt
      421 geidiadldd eeldsyimse keaqfkhnlw nkvnaeyleq qkereekrqk ekeegkpekk
      481 rrrstkrnkt qapansagea iekmlqekki sskinyevlk slnvslttln keqqkieepv
      541 esprvdvdna assskvlpkl qdkptittar kshsvkvklq ikeakeeneq kpveietdlv
      601 eptlpkinac dmvdetddyi deeveadpte mtvgqmlgrh gsedendfgy gydeedy
//
LOCUS       XP_034186515             657 aa            linear   INV 03-SEP-2025
DEFINITION  brf RNA polymerase III subunit isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186515
VERSION     XP_034186515.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330624.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..657
                     /product="brf RNA polymerase III subunit isoform X1"
                     /calculated_mol_wt=74275
     Region          5..40
                     /region_name="TF_Zn_Ribbon"
                     /note="TFIIB zinc-binding; pfam08271"
                     /db_xref="CDD:400527"
     Region          83..173
                     /region_name="CYCLIN_TFIIIB90_rpt1"
                     /note="first cyclin box found in transcription factor IIIB
                     90 kDa subunit (TFIIIB90) and similar proteins; cd20553"
                     /db_xref="CDD:410256"
     Site            order(84,123..126,160,164,167..168)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410256"
     Region          178..269
                     /region_name="CYCLIN_TFIIIB90_rpt2"
                     /note="second cyclin box found in transcription factor
                     IIIB 90 kDa subunit (TFIIIB90) and similar proteins;
                     cd20554"
                     /db_xref="CDD:410257"
     Site            order(221..223,254..255,257..258)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410257"
     Region          427..523
                     /region_name="BRF1"
                     /note="Brf1-like TBP-binding domain; pfam07741"
                     /db_xref="CDD:462250"
     CDS             1..657
                     /gene="Brf"
                     /coded_by="XM_034330624.2:81..2054"
                     /db_xref="GeneID:117607209"
ORIGIN      
        1 msgskcrncg steietdpar gdavctecgf vledqlivse tafketpsgn mmvlgqfvan
       61 dstggatefg atyhingkes rgitlqnark githlcmqlr lnqhcidtsm nfykmalnrh
      121 ltrgrkqahn haacvyitcr tegtahmlid isdvlqicvh elgrtylrft qalcinipsv
      181 dpclyilrfa nklefgekth evsmtalrvv qrmkrdsihs grrpsglcga allmaarlhe
      241 fnrspadiik ivkvhestlr krliefgdtp ssaltleefm tvdleeeqdp pafkaarkkd
      301 rerlqkleni yteinelqae idkqleehsv gktrkrkdav cleredterf vresnlnvik
      361 syvedaddpd seiqesnlnn tnnrlitglg pniasmglis andrenetng qtnatyendt
      421 geidiadldd eeldsyimse keaqfkhnlw nkvnaeyleq qkereekrqk ekeegkpekk
      481 rrrstkrnkt qapansagea iekmlqekki sskinyevlk slnvslttln keqqkieepv
      541 esprvdvdna assskvlpkl qdkptittar kshsvkvklq ikeakeeneq kpveietdlv
      601 eptlpkinac dmvdetddyi deeveadpte mtvgqmlgrh gsedendfgy gydeedy
//
LOCUS       XP_034186516             656 aa            linear   INV 03-SEP-2025
DEFINITION  brf RNA polymerase III subunit isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186516
VERSION     XP_034186516.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330625.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..656
                     /product="brf RNA polymerase III subunit isoform X2"
                     /calculated_mol_wt=74175
     Region          5..40
                     /region_name="TF_Zn_Ribbon"
                     /note="TFIIB zinc-binding; pfam08271"
                     /db_xref="CDD:400527"
     Region          83..173
                     /region_name="CYCLIN_TFIIIB90_rpt1"
                     /note="first cyclin box found in transcription factor IIIB
                     90 kDa subunit (TFIIIB90) and similar proteins; cd20553"
                     /db_xref="CDD:410256"
     Site            order(84,123..126,160,164,167..168)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410256"
     Region          178..269
                     /region_name="CYCLIN_TFIIIB90_rpt2"
                     /note="second cyclin box found in transcription factor
                     IIIB 90 kDa subunit (TFIIIB90) and similar proteins;
                     cd20554"
                     /db_xref="CDD:410257"
     Site            order(221..223,254..255,257..258)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410257"
     Region          427..523
                     /region_name="BRF1"
                     /note="Brf1-like TBP-binding domain; pfam07741"
                     /db_xref="CDD:462250"
     CDS             1..656
                     /gene="Brf"
                     /coded_by="XM_034330625.2:305..2275"
                     /db_xref="GeneID:117607209"
ORIGIN      
        1 msgskcrncg steietdpar gdavctecgf vledqlivse tafketpsgn mmvlgqfvan
       61 dstggatefg atyhingkes rgitlqnark githlcmqlr lnqhcidtsm nfykmalnrh
      121 ltrgrkqahn haacvyitcr tegtahmlid isdvlqicvh elgrtylrft qalcinipsv
      181 dpclyilrfa nklefgekth evsmtalrvv qrmkrdsihs grrpsglcga allmaarlhe
      241 fnrspadiik ivkvhestlr krliefgdtp ssaltleefm tvdleeeqdp pafkaarkkd
      301 rerlqkleni yteinelqae idkqleehsv gktrkrkdav cleredterf vresnlnvik
      361 syvedaddpd seiqesnlnn tnnrlitglg pniasmglis andrenetng qtnatyendt
      421 geidiadldd eeldsyimse keaqfkhnlw nkvnaeyleq qkereekrqk ekeegkpekk
      481 rrrstkrnkt qapansagea iekmlqekki sskinyevlk slnvslttln keqqkieepv
      541 esprvdvdna assskvlpkl qdkptittar kshsvkvklq ikeakeeneq kpveietdlv
      601 eptlpkinac dmdetddyid eeveadptem tvgqmlgrhg sedendfgyg ydeedy
//
LOCUS       XP_034186517             656 aa            linear   INV 03-SEP-2025
DEFINITION  brf RNA polymerase III subunit isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186517
VERSION     XP_034186517.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330626.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..656
                     /product="brf RNA polymerase III subunit isoform X3"
                     /calculated_mol_wt=74204
     Region          5..40
                     /region_name="TF_Zn_Ribbon"
                     /note="TFIIB zinc-binding; pfam08271"
                     /db_xref="CDD:400527"
     Region          83..173
                     /region_name="CYCLIN_TFIIIB90_rpt1"
                     /note="first cyclin box found in transcription factor IIIB
                     90 kDa subunit (TFIIIB90) and similar proteins; cd20553"
                     /db_xref="CDD:410256"
     Site            order(84,123..126,160,164,167..168)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410256"
     Region          178..269
                     /region_name="CYCLIN_TFIIIB90_rpt2"
                     /note="second cyclin box found in transcription factor
                     IIIB 90 kDa subunit (TFIIIB90) and similar proteins;
                     cd20554"
                     /db_xref="CDD:410257"
     Site            order(221..223,254..255,257..258)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:410257"
     Region          426..522
                     /region_name="BRF1"
                     /note="Brf1-like TBP-binding domain; pfam07741"
                     /db_xref="CDD:462250"
     CDS             1..656
                     /gene="Brf"
                     /coded_by="XM_034330626.2:306..2276"
                     /db_xref="GeneID:117607209"
ORIGIN      
        1 msgskcrncg steietdpar gdavctecgf vledqlivse tafketpsgn mmvlgqfvan
       61 dstggatefg atyhingkes rgitlqnark githlcmqlr lnqhcidtsm nfykmalnrh
      121 ltrgrkqahn haacvyitcr tegtahmlid isdvlqicvh elgrtylrft qalcinipsv
      181 dpclyilrfa nklefgekth evsmtalrvv qrmkrdsihs grrpsglcga allmaarlhe
      241 fnrspadiik ivkvhestlr krliefgdtp ssaltleefm tvdleeeqdp pafkaarkkd
      301 rerlqkleni yteinelqae idkqleehsv gktrkrkdvc leredterfv resnlnviks
      361 yvedaddpds eiqesnlnnt nnrlitglgp niasmglisa ndrenetngq tnatyendtg
      421 eidiadldde eldsyimsek eaqfkhnlwn kvnaeyleqq kereekrqke keegkpekkr
      481 rrstkrnktq apansageai ekmlqekkis skinyevlks lnvslttlnk eqqkieepve
      541 sprvdvdnaa ssskvlpklq dkptittark shsvkvklqi keakeeneqk pveietdlve
      601 ptlpkinacd mvdetddyid eeveadptem tvgqmlgrhg sedendfgyg ydeedy
//
LOCUS       XP_034186525             525 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain containing protein 3 lute isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186525
VERSION     XP_034186525.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330634.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..525
                     /product="BTB/POZ domain containing protein 3 lute isoform
                     X1"
                     /calculated_mol_wt=58426
     Region          111..218
                     /region_name="BTB_POZ_BTBD3_6"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing proteins, BTBD3 and BTBD6; cd18282"
                     /db_xref="CDD:349591"
     Region          218..312
                     /region_name="BACK_BTBD3_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing proteins, BTBD3 and BTBD6;
                     cd18488"
                     /db_xref="CDD:350563"
     Region          374..519
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     CDS             1..525
                     /gene="lute"
                     /coded_by="XM_034330634.2:105..1682"
                     /db_xref="GeneID:117607213"
ORIGIN      
        1 mamsnlyski stkpmkrfqd nvtvkqtnpw lnaespitvs dspptvsplf tssivlqreg
       61 tinqplsapa splsslsspi tqlnlssped ctqdpnwqat kptvrernaa mfnnhlmadi
      121 ifivgspght qtipahkyvl atgssvfyam fygglaenkr dievpdvepa aflallrymy
      181 cdevqleadt vlatlyvakk yivphlarac vnyletslta knaclllsqs rlfeepdlmq
      241 rcwevidaqa emalrsdgfv didihtlesv lsretlncke ihiwdaalrw ataecirqel
      301 eptatnqrrl lgsalylirl pamnleefan saaqtgiltq qetidvflhf tasnkpqlcf
      361 ptkprqglkt qvchrfqsca yrsnqwryrg rcdsiqfsvd krifvvgfgl ygsssgaady
      421 nvrielkrlg cvlsenstkf fsdgssstfh vyfenpiqie peyfytasvi ldggelsyfg
      481 qegmseitvg nvnfqfqcss estngtgvqg gqipelifyg ppvdd
//
LOCUS       XP_034186526             501 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain containing protein 3 lute isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186526
VERSION     XP_034186526.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330635.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..501
                     /product="BTB/POZ domain containing protein 3 lute isoform
                     X2"
                     /calculated_mol_wt=55597
     Region          87..194
                     /region_name="BTB_POZ_BTBD3_6"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing proteins, BTBD3 and BTBD6; cd18282"
                     /db_xref="CDD:349591"
     Region          194..288
                     /region_name="BACK_BTBD3_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing proteins, BTBD3 and BTBD6;
                     cd18488"
                     /db_xref="CDD:350563"
     Region          350..495
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     CDS             1..501
                     /gene="lute"
                     /coded_by="XM_034330635.2:286..1791"
                     /db_xref="GeneID:117607213"
ORIGIN      
        1 mmysngkiae spitvsdspp tvsplftssi vlqregtinq plsapaspls slsspitqln
       61 lsspedctqd pnwqatkptv rernaamfnn hlmadiifiv gspghtqtip ahkyvlatgs
      121 svfyamfygg laenkrdiev pdvepaafla llrymycdev qleadtvlat lyvakkyivp
      181 hlaracvnyl etsltaknac lllsqsrlfe epdlmqrcwe vidaqaemal rsdgfvdidi
      241 htlesvlsre tlnckeihiw daalrwatae cirqelepta tnqrrllgsa lylirlpamn
      301 leefansaaq tgiltqqeti dvflhftasn kpqlcfptkp rqglktqvch rfqscayrsn
      361 qwryrgrcds iqfsvdkrif vvgfglygss sgaadynvri elkrlgcvls enstkffsdg
      421 ssstfhvyfe npiqiepeyf ytasvildgg elsyfgqegm seitvgnvnf qfqcssestn
      481 gtgvqggqip elifygppvd d
//
LOCUS       XP_034186527             498 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain containing protein 3 lute isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186527
VERSION     XP_034186527.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330636.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..498
                     /product="BTB/POZ domain containing protein 3 lute isoform
                     X3"
                     /calculated_mol_wt=55299
     Region          84..191
                     /region_name="BTB_POZ_BTBD3_6"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing proteins, BTBD3 and BTBD6; cd18282"
                     /db_xref="CDD:349591"
     Region          191..285
                     /region_name="BACK_BTBD3_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing proteins, BTBD3 and BTBD6;
                     cd18488"
                     /db_xref="CDD:350563"
     Region          347..492
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     CDS             1..498
                     /gene="lute"
                     /coded_by="XM_034330636.2:288..1784"
                     /db_xref="GeneID:117607213"
ORIGIN      
        1 mmysnaespi tvsdspptvs plftssivlq regtinqpls apasplssls spitqlnlss
       61 pedctqdpnw qatkptvrer naamfnnhlm adiifivgsp ghtqtipahk yvlatgssvf
      121 yamfygglae nkrdievpdv epaaflallr ymycdevqle adtvlatlyv akkyivphla
      181 racvnylets ltaknaclll sqsrlfeepd lmqrcwevid aqaemalrsd gfvdidihtl
      241 esvlsretln ckeihiwdaa lrwataecir qeleptatnq rrllgsalyl irlpamnlee
      301 fansaaqtgi ltqqetidvf lhftasnkpq lcfptkprqg lktqvchrfq scayrsnqwr
      361 yrgrcdsiqf svdkrifvvg fglygsssga adynvrielk rlgcvlsens tkffsdgsss
      421 tfhvyfenpi qiepeyfyta svildggels yfgqegmsei tvgnvnfqfq cssestngtg
      481 vqggqipeli fygppvdd
//
LOCUS       XP_034186528             415 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain containing protein 3 lute isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186528
VERSION     XP_034186528.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330637.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..415
                     /product="BTB/POZ domain containing protein 3 lute isoform
                     X4"
                     /calculated_mol_wt=46459
     Region          1..108
                     /region_name="BTB_POZ_BTBD3_6"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing proteins, BTBD3 and BTBD6; cd18282"
                     /db_xref="CDD:349591"
     Region          108..202
                     /region_name="BACK_BTBD3_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing proteins, BTBD3 and BTBD6;
                     cd18488"
                     /db_xref="CDD:350563"
     Region          264..409
                     /region_name="PHR"
                     /note="PHR domain; pfam08005"
                     /db_xref="CDD:462339"
     CDS             1..415
                     /gene="lute"
                     /coded_by="XM_034330637.2:459..1706"
                     /db_xref="GeneID:117607213"
ORIGIN      
        1 mfnnhlmadi ifivgspght qtipahkyvl atgssvfyam fygglaenkr dievpdvepa
       61 aflallrymy cdevqleadt vlatlyvakk yivphlarac vnyletslta knaclllsqs
      121 rlfeepdlmq rcwevidaqa emalrsdgfv didihtlesv lsretlncke ihiwdaalrw
      181 ataecirqel eptatnqrrl lgsalylirl pamnleefan saaqtgiltq qetidvflhf
      241 tasnkpqlcf ptkprqglkt qvchrfqsca yrsnqwryrg rcdsiqfsvd krifvvgfgl
      301 ygsssgaady nvrielkrlg cvlsenstkf fsdgssstfh vyfenpiqie peyfytasvi
      361 ldggelsyfg qegmseitvg nvnfqfqcss estngtgvqg gqipelifyg ppvdd
//
LOCUS       XP_076548223             375 aa            linear   INV 03-SEP-2025
DEFINITION  BTB/POZ domain containing protein 3 lute isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548223
VERSION     XP_076548223.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692108.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..375
                     /product="BTB/POZ domain containing protein 3 lute isoform
                     X5"
                     /calculated_mol_wt=41707
     Region          111..218
                     /region_name="BTB_POZ_BTBD3_6"
                     /note="BTB (Broad-Complex, Tramtrack and Bric a brac)/POZ
                     (poxvirus and zinc finger) domain found in BTB/POZ
                     domain-containing proteins, BTBD3 and BTBD6; cd18282"
                     /db_xref="CDD:349591"
     Region          218..312
                     /region_name="BACK_BTBD3_like"
                     /note="BACK (BTB and C-terminal Kelch) domain found in
                     BTB/POZ domain-containing proteins, BTBD3 and BTBD6;
                     cd18488"
                     /db_xref="CDD:350563"
     CDS             1..375
                     /gene="lute"
                     /coded_by="XM_076692108.1:107..1234"
                     /db_xref="GeneID:117607213"
ORIGIN      
        1 mamsnlyski stkpmkrfqd nvtvkqtnpw lnaespitvs dspptvsplf tssivlqreg
       61 tinqplsapa splsslsspi tqlnlssped ctqdpnwqat kptvrernaa mfnnhlmadi
      121 ifivgspght qtipahkyvl atgssvfyam fygglaenkr dievpdvepa aflallrymy
      181 cdevqleadt vlatlyvakk yivphlarac vnyletslta knaclllsqs rlfeepdlmq
      241 rcwevidaqa emalrsdgfv didihtlesv lsretlncke ihiwdaalrw ataecirqel
      301 eptatnqrrl lgsalylirl pamnleefan saaqtgiltq qetidvflhf tasnkpqlcf
      361 ptkprqglkt qikvi
//
LOCUS       XP_034186529             453 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin gamma-2 chain isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186529
VERSION     XP_034186529.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330638.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..453
                     /product="tubulin gamma-2 chain isoform X1"
                     /calculated_mol_wt=51193
     Region          4..435
                     /region_name="gamma_tubulin"
                     /note="The gamma-tubulin family; cd02188"
                     /db_xref="CDD:276957"
     Site            order(11..13,16,102,140,142..146,171,173,207,225,228..229)
                     /site_type="other"
                     /note="nucleotide binding site [chemical binding]"
                     /db_xref="CDD:276957"
     Site            order(47,133,252,254,258,261..262,264..265,330..331,
                     333..334,337..338,341,353..354,357..358,430,433..434)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:276957"
     CDS             1..453
                     /gene="LOC117607215"
                     /coded_by="XM_034330638.2:181..1542"
                     /db_xref="GeneID:117607215"
ORIGIN      
        1 mpcevitlql gqcgnqigfe fwkklcaehg ispegiledy avdgtdrkdv ffyqsddehy
       61 ipravlldle prvihtimns qysklynpen iflskhggga gnnwasgyhq geklqeeifd
      121 ildreadgsd slegfvlchs iaggtgsgmg sfmlesladr fpkklietys vfpnqdeisd
      181 vvvqpynsll tlkrltqcad cvvvldntal nriasdrlhi qnpsftqink lvstimsvst
      241 ttlrypsymn ndlvgliapl iptprlhflm tgytplttdq egasvrktsv ldvmrrllqp
      301 knmmvstald rnashcyisi lniiqgevdp tqvhkslqri rerklaqfip wgpasiqval
      361 srkspyiqst hrvsglmlan htnisslfdr alqqydklrk reafleqfrk ekmfednlde
      421 ldnsrevvey lvkeyqaatr vdylswnpnk adv
//
LOCUS       XP_034186530             376 aa            linear   INV 03-SEP-2025
DEFINITION  tubulin gamma-1 chain isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186530
VERSION     XP_034186530.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330639.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="tubulin gamma-1 chain isoform X2"
                     /calculated_mol_wt=42386
     Region          1..358
                     /region_name="gamma_tubulin"
                     /note="The gamma-tubulin family; cd02188"
                     /db_xref="CDD:276957"
     Site            order(56,175,177,181,184..185,187..188,253..254,256..257,
                     260..261,264,276..277,280..281,353,356..357)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:276957"
     CDS             1..376
                     /gene="LOC117607215"
                     /coded_by="XM_034330639.2:306..1436"
                     /db_xref="GeneID:117607215"
ORIGIN      
        1 mnsqysklyn peniflskhg ggagnnwasg yhqgeklqee ifdildread gsdslegfvl
       61 chsiaggtgs gmgsfmlesl adrfpkklie tysvfpnqde isdvvvqpyn slltlkrltq
      121 cadcvvvldn talnriasdr lhiqnpsftq inklvstims vstttlryps ymnndlvgli
      181 apliptprlh flmtgytplt tdqegasvrk tsvldvmrrl lqpknmmvst aldrnashcy
      241 isilniiqge vdptqvhksl qrirerklaq fipwgpasiq valsrkspyi qsthrvsglm
      301 lanhtnissl fdralqqydk lrkreafleq frkekmfedn ldeldnsrev veylvkeyqa
      361 atrvdylswn pnkadv
//
LOCUS       XP_034186532             396 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA methyltransferase roswell [Osmia lignaria lignaria].
ACCESSION   XP_034186532
VERSION     XP_034186532.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330641.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186532.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="tRNA methyltransferase roswell"
                     /calculated_mol_wt=47086
     Region          190..369
                     /region_name="Trm10_MRRP1"
                     /note="Mitochondrial ribonuclease P protein 1; cd18102"
                     /db_xref="CDD:349975"
     Site            order(278..280,297..298,300,302,323..324,326,339..340,342,
                     345)
                     /site_type="other"
                     /note="SAM binding site [chemical binding]"
                     /db_xref="CDD:349975"
     CDS             1..396
                     /gene="rswl"
                     /coded_by="XM_034330641.2:283..1473"
                     /db_xref="GeneID:117607216"
ORIGIN      
        1 mifkmynrvf qcyvtatakl ycngvvypln ykfqfsaqnh allaraycts ksqkilqeye
       61 qmyeqqlnek lkdpnfaaky nkcklevedy rsrlfripdv ikpydwllll dtdfrgqrrt
      121 yleyrwktem keandikrre ikkkqiekkr eeidpntyrl shstlcyrir dqainhfyng
      181 rlmsamlhsp pivldfsydq ymspserqlc akqllltfsm nrvhddpcnl yfcnvnrqsl
      241 tmqklhitip tiydtdfpln itsksyleif dksrlvyltp dsnqimthyd pnsvyiigal
      301 vdkvntqpcs aqkarregik mvrlplaekl qwgigstktl plnhmlsill dlkytndwni
      361 alqhiprrkl keerekavln rfkkrqelie sliknn
//
LOCUS       XP_034186535             342 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607218 [Osmia lignaria lignaria].
ACCESSION   XP_034186535
VERSION     XP_034186535.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330644.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..342
                     /product="uncharacterized protein LOC117607218"
                     /calculated_mol_wt=38126
     CDS             1..342
                     /gene="LOC117607218"
                     /coded_by="XM_034330644.2:191..1219"
                     /db_xref="GeneID:117607218"
ORIGIN      
        1 mfdsnkitel ddddfqaqkn kdreiragrs kcgnkysytk tkkswkkrli kciindlkny
       61 gptnvmhika lanitgkpir iftsdgnlyq tincnrndrk miqcdaidie yhkekhwtll
      121 gnkdpaeiek dlnaclfvvi aaqtgknstd lrnstidylt nnanylieei dkissddnal
      181 lmiggaryng tspraagiil ddsqnvlcdg crevghprgh asdkdatgpi dsvenysrtt
      241 gsrksgflsr ndqnlvahya lchesaqqam nslnngefsl aitllgkelp talpkmkefv
      301 ngeaftgels iirvtvvlrh hqgkrndpda nvfvhtiypr ss
//
LOCUS       XP_034186536             342 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607218 [Osmia lignaria lignaria].
ACCESSION   XP_034186536
VERSION     XP_034186536.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330645.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..342
                     /product="uncharacterized protein LOC117607218"
                     /calculated_mol_wt=38126
     CDS             1..342
                     /gene="LOC117607218"
                     /coded_by="XM_034330645.2:189..1217"
                     /db_xref="GeneID:117607218"
ORIGIN      
        1 mfdsnkitel ddddfqaqkn kdreiragrs kcgnkysytk tkkswkkrli kciindlkny
       61 gptnvmhika lanitgkpir iftsdgnlyq tincnrndrk miqcdaidie yhkekhwtll
      121 gnkdpaeiek dlnaclfvvi aaqtgknstd lrnstidylt nnanylieei dkissddnal
      181 lmiggaryng tspraagiil ddsqnvlcdg crevghprgh asdkdatgpi dsvenysrtt
      241 gsrksgflsr ndqnlvahya lchesaqqam nslnngefsl aitllgkelp talpkmkefv
      301 ngeaftgels iirvtvvlrh hqgkrndpda nvfvhtiypr ss
//
LOCUS       XP_034186537             342 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607218 [Osmia lignaria lignaria].
ACCESSION   XP_034186537
VERSION     XP_034186537.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330646.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..342
                     /product="uncharacterized protein LOC117607218"
                     /calculated_mol_wt=38126
     CDS             1..342
                     /gene="LOC117607218"
                     /coded_by="XM_034330646.2:172..1200"
                     /db_xref="GeneID:117607218"
ORIGIN      
        1 mfdsnkitel ddddfqaqkn kdreiragrs kcgnkysytk tkkswkkrli kciindlkny
       61 gptnvmhika lanitgkpir iftsdgnlyq tincnrndrk miqcdaidie yhkekhwtll
      121 gnkdpaeiek dlnaclfvvi aaqtgknstd lrnstidylt nnanylieei dkissddnal
      181 lmiggaryng tspraagiil ddsqnvlcdg crevghprgh asdkdatgpi dsvenysrtt
      241 gsrksgflsr ndqnlvahya lchesaqqam nslnngefsl aitllgkelp talpkmkefv
      301 ngeaftgels iirvtvvlrh hqgkrndpda nvfvhtiypr ss
//
LOCUS       XP_034186540             323 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA methyltransferase 61 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186540
VERSION     XP_034186540.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330649.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186540.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..323
                     /product="tRNA methyltransferase 61 isoform X1"
                     /calculated_mol_wt=36480
     Region          72..307
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..323
                     /gene="Trmt61"
                     /coded_by="XM_034330649.2:1..972"
                     /db_xref="GeneID:117607220"
ORIGIN      
        1 msfnkvkeii eegdvvilyl gpsnmhslei tqkilskkgq mvdnvfqtiy galkvfslvg
       61 qmygtkvqls rgwgyvlqpt pelwtltvph rtqiiyspdi sliiylmdla pgsiiietgt
      121 gsgslshali rtirphghly tfdfheqrvn vakiefekhg lskfvtlyhk dvcmegfgeq
      181 lrnkadaifl dlphpwlaid ralcalkesg gklcsfspci eqvqrtcekl tsigfielkt
      241 yeclqremnv qyknlpildl eclkhehvnk dmaqknenek qeqtklltvt hahslpghtg
      301 fitiatlppv yarktdlnle skd
//
LOCUS       XP_076548224             289 aa            linear   INV 03-SEP-2025
DEFINITION  tRNA methyltransferase 61 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548224
VERSION     XP_076548224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692109.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..289
                     /product="tRNA methyltransferase 61 isoform X2"
                     /calculated_mol_wt=32656
     Region          38..273
                     /region_name="AdoMet_MTases"
                     /note="S-adenosylmethionine-dependent methyltransferases
                     (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes
                     that use S-adenosyl-L-methionine (SAM or AdoMet) as a
                     substrate for methyltransfer, creating the product
                     S-adenosyl-L-homocysteine (AdoHcy); cl17173"
                     /db_xref="CDD:473071"
     CDS             1..289
                     /gene="Trmt61"
                     /coded_by="XM_076692109.1:133..1002"
                     /db_xref="GeneID:117607220"
ORIGIN      
        1 mckkgqmvdn vfqtiygalk vfslvgqmyg tkvqlsrgwg yvlqptpelw tltvphrtqi
       61 iyspdislii ylmdlapgsi iietgtgsgs lshalirtir phghlytfdf heqrvnvaki
      121 efekhglskf vtlyhkdvcm egfgeqlrnk adaifldlph pwlaidralc alkesggklc
      181 sfspcieqvq rtcekltsig fielktyecl qremnvqykn lpildleclk hehvnkdmaq
      241 knenekqeqt klltvthahs lpghtgfiti atlppvyark tdlnleskd
//
LOCUS       XP_034186543             221 aa            linear   INV 03-SEP-2025
DEFINITION  peroxiredoxin 6a [Osmia lignaria lignaria].
ACCESSION   XP_034186543
VERSION     XP_034186543.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330652.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..221
                     /product="peroxiredoxin 6a"
                     /calculated_mol_wt=24801
     Region          4..217
                     /region_name="PRX_1cys"
                     /note="Peroxiredoxin (PRX) family, 1-cys PRX subfamily;
                     composed of PRXs containing only one conserved cysteine,
                     which serves as the peroxidatic cysteine. They are
                     homodimeric thiol-specific antioxidant (TSA) proteins that
                     confer a protective role in cells by...; cd03016"
                     /db_xref="CDD:239314"
     Site            order(4,42,45..47,50,82,85,111,126,140..142,144..146,
                     148..153,155,158,162..163,172..173,175..176,212,215)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239314"
     Site            order(16,70..72,74..75,77..78,80..81,93,102..103,113,
                     124..125,188,205)
                     /site_type="other"
                     /note="decamer (pentamer of dimers) interface [polypeptide
                     binding]"
                     /db_xref="CDD:239314"
     Site            order(41,44,128)
                     /site_type="active"
                     /note="catalytic triad [active]"
                     /db_xref="CDD:239314"
     CDS             1..221
                     /gene="Prx6a"
                     /coded_by="XM_034330652.2:55..720"
                     /db_xref="GeneID:117607223"
ORIGIN      
        1 mvllgetfpn fvaetqigqi nfhdwlsdsw gilfshpndf tpvcttelar vvklipefeq
       61 lgvkvialsc nsvdshrkwi edikafaqvt dkefpypiie detrklatll gmldpaevds
      121 kgipltarav fiidpakkmr lsilypattg rsfdeilrvi kslqltekhk vatpvdwkng
      181 dhvmiqptvs ddeakklydn ieiltlpsgk nylrmvpqpa i
//
LOCUS       XP_034186551             192 aa            linear   INV 03-SEP-2025
DEFINITION  jagunal [Osmia lignaria lignaria].
ACCESSION   XP_034186551
VERSION     XP_034186551.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330660.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..192
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..192
                     /product="jagunal"
                     /calculated_mol_wt=22350
     Region          1..187
                     /region_name="Jagunal"
                     /note="ER re-organization during oogenesis; pfam07086"
                     /db_xref="CDD:462086"
     CDS             1..192
                     /gene="jagn"
                     /coded_by="XM_034330660.2:50..628"
                     /db_xref="GeneID:117607226"
ORIGIN      
        1 maskvtlsqa lgtdgsdfnh rqkiashyqi satnksrlky ciffhyllff vmlaklsadi
       61 ldhldvfile ieelqvpqpl wweyiwcasl mlsflalsai krnkiktlrq ymigiillgy
      121 gpllyaviyy fkdvwkylti gksdeihfwq glpygllwya fillasqvht fslyfswnll
      181 vawktrgskk ad
//
LOCUS       XP_034186802             299 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607334 [Osmia lignaria lignaria].
ACCESSION   XP_034186802
VERSION     XP_034186802.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330911.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..299
                     /product="uncharacterized protein LOC117607334"
                     /calculated_mol_wt=32069
     Region          92..154
                     /region_name="bHLH_TS_dAS-C_like"
                     /note="basic helix-loop-helix (bHLH) domain found in
                     Drosophila melanogaster achaete-scute complex (AS-C)
                     proteins and similar proteins; cd19744"
                     /db_xref="CDD:381587"
     Site            order(96..97,100..101,103..104,137,140,144,146..147,151,
                     153..154)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:381587"
     CDS             1..299
                     /gene="LOC117607334"
                     /coded_by="XM_034330911.2:304..1203"
                     /db_xref="GeneID:117607334"
ORIGIN      
        1 mtlvgttegn vlpimlsvqq qhhhhnlhgs ttsaqthrgn vivstssmpt ndikiqqhgc
       61 krskiysqat gpygtvphqp asvarrnare rnrvkqvnng fatlrqhipq svaqalggst
      121 agthggsrag skklskvetl rmaveyirsl qrlleehdsg sdlasvsspt ssppsstssa
      181 sstcssntgl gvdanhhsae lrlrgnvnnd irhhnnsdlh rhqhirqnps ptfvpapcse
      241 asssptpsfv seassagsqg ygtsgtlytt hsdgydnyep mspedeelld viswwqqsq
//
LOCUS       XP_034190769             226 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609042 [Osmia lignaria lignaria].
ACCESSION   XP_034190769
VERSION     XP_034190769.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334878.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..226
                     /product="uncharacterized protein LOC117609042"
                     /calculated_mol_wt=25908
     Region          49..>135
                     /region_name="DUF4818"
                     /note="Domain of unknown function (DUF4818); pfam16089"
                     /db_xref="CDD:465013"
     CDS             1..226
                     /gene="LOC117609042"
                     /coded_by="XM_034334878.2:359..1039"
                     /db_xref="GeneID:117609042"
ORIGIN      
        1 mdmetvkeyl afinphwiav vatgmythlr qiciiglcfd edttlpfdps yasvifvfsv
       61 lcllaeyrlw prtlkeppiq llyiyemlva alitnlttra iwvplmhain cltqessrwl
      121 lwlnatlgls hyslltdfay ymakdcaamh mafcmsilsf vwmldatesl daildtrake
      181 qnggfwsict ckrrkisfan pevtdwfvyk fmcsadpvne nkqsds
//
LOCUS       XP_034190772             208 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609043 [Osmia lignaria lignaria].
ACCESSION   XP_034190772
VERSION     XP_034190772.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034334881.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..208
                     /product="uncharacterized protein LOC117609043"
                     /calculated_mol_wt=23902
     Region          40..>101
                     /region_name="DUF4818"
                     /note="Domain of unknown function (DUF4818); pfam16089"
                     /db_xref="CDD:465013"
     CDS             1..208
                     /gene="LOC117609043"
                     /coded_by="XM_034334881.2:321..947"
                     /db_xref="GeneID:117609043"
ORIGIN      
        1 mvqllhtiwa lllnalelpi frtcfaname dedresllcg ppfssflfsl giymiiihst
       61 lfprsfrepe mgflvlyefl atvflleftl aciwtpldvl istrlpttvc hllqqfgsdd
      121 avisfltsks ltviitymva itfllltlyv tdsvdytilr dcgampfamd vkerlwdrlq
      181 relpflnket ktcrckvrrr grskshlk
//
LOCUS       XP_076548225             326 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306063 [Osmia lignaria lignaria].
ACCESSION   XP_076548225
VERSION     XP_076548225.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692110.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..326
                     /product="uncharacterized protein LOC143306063"
                     /calculated_mol_wt=37889
     CDS             1..326
                     /gene="LOC143306063"
                     /coded_by="XM_076692110.1:72..1052"
                     /db_xref="GeneID:143306063"
ORIGIN      
        1 mncaafmfde iryelngvei drcknvgitt mikngvsltt trskmlenag widipnaggy
       61 fdfcvplnml lgfcedykri vinahhelil irsrddknai fgtdgakytl qlykvqwrmp
      121 hvtleerhkl sllrilengq tiimsyrswd lyeypmlqat tkhtwsikaa tqlekpryvi
      181 falqtdkknr lnrhitkfdd ckltnfrlyl nsdsypydnl nldfnknrys vlydmyakfr
      241 ksyygamcsn gddgalydki lffgygpfvv idcshqnesi ksatidvrid fecsenvpen
      301 tsaycliihd rivqynpltn vvrkls
//
LOCUS       XP_034183071            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183071
VERSION     XP_034183071.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327180.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183071.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327180.2:1794..10097"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183072            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183072
VERSION     XP_034183072.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327181.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183072.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327181.2:1865..10168"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183073            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183073
VERSION     XP_034183073.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327182.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183073.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327182.2:2703..11006"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183074            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183074
VERSION     XP_034183074.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327183.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183074.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327183.2:2551..10854"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183070            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183070
VERSION     XP_034183070.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327179.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183070.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327179.2:2051..10354"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183075            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183075
VERSION     XP_034183075.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327184.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183075.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327184.2:6373..14676"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183076            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183076
VERSION     XP_034183076.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327185.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183076.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327185.2:2186..10489"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183077            2767 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183077
VERSION     XP_034183077.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327186.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183077.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2767
                     /product="tet methylcytosine dioxygenase-like isoform X1"
                     /calculated_mol_wt=308405
     Region          324..363
                     /region_name="zf-CXXC"
                     /note="CXXC zinc finger domain; pfam02008"
                     /db_xref="CDD:366873"
     Region          1564..1883
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2480..>2605
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2601..2675
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2767
                     /gene="Tet"
                     /coded_by="XM_034327186.2:1568..9871"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 msaevtkevv atervagspp aavatspssv gpgdparhlp pfssfagdna mdssttlttl
       61 hpqdvglglt sswdyyeglt grlidsrgev vlasqyrpwe sknaaggggp pgeglpsfas
      121 qftapseaep qltaltplsp asishsppvt savglpsfht lgtplpaphp hrgsvgyplv
      181 papvqarevp alqqqllder hiqllgsspv qafpppppgh phhtvltvvk peypqlhhan
      241 fqnpmstvld ssptsrpigi dgrkkerrki ragsmeseds agagnvessg qvaavsstan
      301 rgphhlgggi adadgdssls dkpakkkrkr cgecigcqrk dncgdcapcr ndkshqickm
      361 rrcekltekk hlyhlqtgeg afrergrgrg kgatrsyrki arqvstpdsa paglgspqag
      421 igglqqhqqq pqqqtqqtlh qpdpmqqskd nlnpaanqqt galrngnppp pvvsmspgvm
      481 pfygdsgagf rpaagfgntv whqgvtpvgt vavetsaapw ppqqfiqqip apnqleelry
      541 htqytaaapy hpqpvayqqq tfittdqsaf qragatgqyt itgqpsplpp vapqtvpsta
      601 qrplngqfmd paatatqsvg yerqdyvngy qqqdvtmapp psttptsrss svhmdnqqqq
      661 qasqqapsqp qqqqtdtqvk gfatiaasnv sgnempgypl qpgpqslhns ngypgyvemg
      721 qqqvqnqwqp qvaqqqshqq qqqqqhqhmq rqhsvsdagq rhiwsdtstg akmnnvsnsm
      781 nwsdgtlqqq qqqhqqqksq qqqqqssmqs nsmkqqeqqq qqsmqmqgqq elprpashms
      841 wengsvketp htpqswtdnt dnqqqqtqgn wsrqspklrd tqnprltpss qqqpqhqgwl
      901 qhspklsdhq qnpshqnarm tpsgqqhswq qsspemqaqq nsnqsnprlt pqqqqqqtqq
      961 qqqqqwsqqt kieknprltp snqmswpdtp tsqsnwspns mksdgsqqpq qqwpdsqpsp
     1021 rrtpghqpaa wqpqpqenkm dnqmswspss vaenqptwgq qttkqdmqns gervlwqqqt
     1081 tdtgkpngfs dnqdaqesnv faqsnrvnln srlksmilnk qqqqqqqqlq hqhlqqqqqq
     1141 qaqqqaqqqa qqqaqqqaqq qaqqqaqqqa qqqaqqqaqq qaahqmshqe qqqqqqhhnm
     1201 hhqmqshhvn snsgtnrdyh sedrmrdshe nsdkmqekqq neqylsqsnm ismtqsnesl
     1261 tgnfllhshh pradslpdgg glwewsgggn ngsvlsengm tviesfmkfg senktvlekp
     1321 cekqqeehqe qqhlwkeemk sndnqtiddk sfqrslcldq tnndkvfeqc ennadnsekc
     1381 enslgfpett qvslineedr pyvestdktt stgtncdssq iikqenvgey gcsssdcvss
     1441 paassksesc ttkeiksepd qrsqensdym frgdggpakv tagtgswccr rggteqptpe
     1501 hlrdgccqgl qtrdeilads adksdvkneg pqsprsgsvp sattklqdhl dklknnvrte
     1561 vpdcncfpad kcppepgsyy thlgaaaslp dlrndlerrt glkgnairfe kviytgkegk
     1621 ttqgcpmakw ilrrsgldek iltivkhrqg hkcptawivv amvawegvpt headriysll
     1681 shklnrfglp ttrrcgtnep rtcacqgldp dtcgasfsfg cswsmyyngc kyarsktvrk
     1741 frlsvrseeq eveermhvla tllsplylsl apeafnnqtq fereasecrl gfkpgrpfsg
     1801 vtacidfcah shrdlhnmnn gctvvvtltk hrtlskpede qlhvlplyim dttdeygske
     1861 gqdekvrdgs vevltkypce vrvrsvplqp crrhgkkrke depdtvsnkk dgskntgekm
     1921 neqgrvpghq eaakpqmrdt qlslemasmf egmdaqlqss qvsstvldsp vsmyqgwgyq
     1981 neqqwgrngw ldqrknnwln pwgeysfggl dreakvdpds tslddtrprs avsqddhrpg
     2041 srnlpnstid sprncyphsp rthpisprss hastpgdvcy smsprgcppt phdqkmaspr
     2101 ssgypdtgyt hvptsrnynv ydnrqgsasp ktgctnfpph ldqrsalgrn hpqhinalrq
     2161 sceqskfpfs kpahdssqqk yhmtqnylea qksqteqsft griqghypsh pvqsvrnhpy
     2221 qnqhdsnmdv ypglsavspc mdnashkafg atnpdlllhs sshlppnnqq ngaqnyspvd
     2281 qrryktqkvp eqrmpgvnhm spapsdqagg wnmlgswypd qskpynqqim yndqiasert
     2341 hqqpvdhqkt lnwndriphp dqpkppcwea ppdpspfrvp kgrppsrtas sqnpnadnty
     2401 qnssnrtflk pqepmrtavy adslsngvqs igtmhqsnqr kpewpeenki kegfhdnrqn
     2461 vanpnsncfp wpeemkqvqd fhpvpalghp hasfpqygpy ptypvfekpy snswegynyh
     2521 qtpyhpqtpe yppqfyqqpk reafvpspqy pyqgvppyqg lnpgwarwet prwdiygppp
     2581 yfpvlpeppp kaeplgevad ysdneecfkd sqmggvaial ghgsvlfeca khemhsttal
     2641 kkpnrlnptr islvfyqhrn lnrprhgwde weekmrlrkl gvtsttgtta ittssttsqs
     2701 lstpstptsp asaiineksi plpyippvps sqfmmrspty ttmtwttlfp mhpcmitgpy
     2761 qeggaig
//
LOCUS       XP_034183078            2378 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183078
VERSION     XP_034183078.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327187.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183078.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2378
                     /product="tet methylcytosine dioxygenase-like isoform X2"
                     /calculated_mol_wt=267437
     Region          1175..1494
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2091..>2216
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2212..2286
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2378
                     /gene="Tet"
                     /coded_by="XM_034327187.2:198..7334"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 meayacsyrk iarqvstpds apaglgspqa gigglqqhqq qpqqqtqqtl hqpdpmqqsk
       61 dnlnpaanqq tgalrngnpp ppvvsmspgv mpfygdsgag frpaagfgnt vwhqgvtpvg
      121 tvavetsaap wppqqfiqqi papnqleelr yhtqytaaap yhpqpvayqq qtfittdqsa
      181 fqragatgqy titgqpsplp pvapqtvpst aqrplngqfm dpaatatqsv gyerqdyvng
      241 yqqqdvtmap ppsttptsrs ssvhmdnqqq qqasqqapsq pqqqqtdtqv kgfatiaasn
      301 vsgnempgyp lqpgpqslhn sngypgyvem gqqqvqnqwq pqvaqqqshq qqqqqqhqhm
      361 qrqhsvsdag qrhiwsdtst gakmnnvsns mnwsdgtlqq qqqqhqqqks qqqqqqssmq
      421 snsmkqqeqq qqqsmqmqgq qelprpashm swengsvket phtpqswtdn tdnqqqqtqg
      481 nwsrqspklr dtqnprltps sqqqpqhqgw lqhspklsdh qqnpshqnar mtpsgqqhsw
      541 qqsspemqaq qnsnqsnprl tpqqqqqqtq qqqqqqwsqq tkieknprlt psnqmswpdt
      601 ptsqsnwspn smksdgsqqp qqqwpdsqps prrtpghqpa awqpqpqenk mdnqmswsps
      661 svaenqptwg qqttkqdmqn sgervlwqqq ttdtgkpngf sdnqdaqesn vfaqsnrvnl
      721 nsrlksmiln kqqqqqqqql qhqhlqqqqq qqaqqqaqqq aqqqaqqqaq qqaqqqaqqq
      781 aqqqaqqqaq qqaahqmshq eqqqqqqhhn mhhqmqshhv nsnsgtnrdy hsedrmrdsh
      841 ensdkmqekq qneqylsqsn mismtqsnes ltgnfllhsh hpradslpdg gglwewsggg
      901 nngsvlseng mtviesfmkf gsenktvlek pcekqqeehq eqqhlwkeem ksndnqtidd
      961 ksfqrslcld qtnndkvfeq cennadnsek censlgfpet tqvslineed rpyvestdkt
     1021 tstgtncdss qiikqenvge ygcsssdcvs spaasskses cttkeiksep dqrsqensdy
     1081 mfrgdggpak vtagtgswcc rrggteqptp ehlrdgccqg lqtrdeilad sadksdvkne
     1141 gpqsprsgsv psattklqdh ldklknnvrt evpdcncfpa dkcppepgsy ythlgaaasl
     1201 pdlrndlerr tglkgnairf ekviytgkeg kttqgcpmak wilrrsglde kiltivkhrq
     1261 ghkcptawiv vamvawegvp theadriysl lshklnrfgl pttrrcgtne prtcacqgld
     1321 pdtcgasfsf gcswsmyyng ckyarsktvr kfrlsvrsee qeveermhvl atllsplyls
     1381 lapeafnnqt qfereasecr lgfkpgrpfs gvtacidfca hshrdlhnmn ngctvvvtlt
     1441 khrtlskped eqlhvlplyi mdttdeygsk egqdekvrdg svevltkypc evrvrsvplq
     1501 pcrrhgkkrk edepdtvsnk kdgskntgek mneqgrvpgh qeaakpqmrd tqlslemasm
     1561 fegmdaqlqs sqvsstvlds pvsmyqgwgy qneqqwgrng wldqrknnwl npwgeysfgg
     1621 ldreakvdpd stslddtrpr savsqddhrp gsrnlpnsti dsprncyphs prthpisprs
     1681 shastpgdvc ysmsprgcpp tphdqkmasp rssgypdtgy thvptsrnyn vydnrqgsas
     1741 pktgctnfpp hldqrsalgr nhpqhinalr qsceqskfpf skpahdssqq kyhmtqnyle
     1801 aqksqteqsf tgriqghyps hpvqsvrnhp yqnqhdsnmd vypglsavsp cmdnashkaf
     1861 gatnpdlllh ssshlppnnq qngaqnyspv dqrryktqkv peqrmpgvnh mspapsdqag
     1921 gwnmlgswyp dqskpynqqi myndqiaser thqqpvdhqk tlnwndriph pdqpkppcwe
     1981 appdpspfrv pkgrppsrta ssqnpnadnt yqnssnrtfl kpqepmrtav yadslsngvq
     2041 sigtmhqsnq rkpewpeenk ikegfhdnrq nvanpnsncf pwpeemkqvq dfhpvpalgh
     2101 phasfpqygp yptypvfekp ysnswegyny hqtpyhpqtp eyppqfyqqp kreafvpspq
     2161 ypyqgvppyq glnpgwarwe tprwdiygpp pyfpvlpepp pkaeplgeva dysdneecfk
     2221 dsqmggvaia lghgsvlfec akhemhstta lkkpnrlnpt rislvfyqhr nlnrprhgwd
     2281 eweekmrlrk lgvtsttgtt aittssttsq slstpstpts pasaiineks iplpyippvp
     2341 ssqfmmrspt yttmtwttlf pmhpcmitgp yqeggaig
//
LOCUS       XP_034183080            2323 aa            linear   INV 03-SEP-2025
DEFINITION  tet methylcytosine dioxygenase-like isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183080
VERSION     XP_034183080.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327189.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183080.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2323
                     /product="tet methylcytosine dioxygenase-like isoform X3"
                     /calculated_mol_wt=261525
     Region          1120..1439
                     /region_name="TET"
                     /note="oxygenase domain of ten-eleven translocation
                     (TET)1, TET2, and TET3 methylcytosine dioxygenases and
                     similar proteins; cd18892"
                     /db_xref="CDD:380671"
     Region          <2036..>2161
                     /region_name="PRK10263"
                     /note="DNA translocase FtsK; Provisional"
                     /db_xref="CDD:236669"
     Region          <2157..2231
                     /region_name="Tet_JBP"
                     /note="oxygenase domain of ten-eleven translocation (TET)
                     enzymes, J-binding proteins (JBPs), and similar proteins;
                     cl40427"
                     /db_xref="CDD:394797"
     CDS             1..2323
                     /gene="Tet"
                     /coded_by="XM_034327189.2:592..7563"
                     /db_xref="GeneID:117605643"
ORIGIN      
        1 mqqskdnlnp aanqqtgalr ngnppppvvs mspgvmpfyg dsgagfrpaa gfgntvwhqg
       61 vtpvgtvave tsaapwppqq fiqqipapnq leelryhtqy taaapyhpqp vayqqqtfit
      121 tdqsafqrag atgqytitgq psplppvapq tvpstaqrpl ngqfmdpaat atqsvgyerq
      181 dyvngyqqqd vtmapppstt ptsrsssvhm dnqqqqqasq qapsqpqqqq tdtqvkgfat
      241 iaasnvsgne mpgyplqpgp qslhnsngyp gyvemgqqqv qnqwqpqvaq qqshqqqqqq
      301 qhqhmqrqhs vsdagqrhiw sdtstgakmn nvsnsmnwsd gtlqqqqqqh qqqksqqqqq
      361 qssmqsnsmk qqeqqqqqsm qmqgqqelpr pashmsweng svketphtpq swtdntdnqq
      421 qqtqgnwsrq spklrdtqnp rltpssqqqp qhqgwlqhsp klsdhqqnps hqnarmtpsg
      481 qqhswqqssp emqaqqnsnq snprltpqqq qqqtqqqqqq qwsqqtkiek nprltpsnqm
      541 swpdtptsqs nwspnsmksd gsqqpqqqwp dsqpsprrtp ghqpaawqpq pqenkmdnqm
      601 swspssvaen qptwgqqttk qdmqnsgerv lwqqqttdtg kpngfsdnqd aqesnvfaqs
      661 nrvnlnsrlk smilnkqqqq qqqqlqhqhl qqqqqqqaqq qaqqqaqqqa qqqaqqqaqq
      721 qaqqqaqqqa qqqaqqqaah qmshqeqqqq qqhhnmhhqm qshhvnsnsg tnrdyhsedr
      781 mrdshensdk mqekqqneqy lsqsnmismt qsnesltgnf llhshhprad slpdggglwe
      841 wsgggnngsv lsengmtvie sfmkfgsenk tvlekpcekq qeehqeqqhl wkeemksndn
      901 qtiddksfqr slcldqtnnd kvfeqcenna dnsekcensl gfpettqvsl ineedrpyve
      961 stdkttstgt ncdssqiikq envgeygcss sdcvsspaas sksescttke iksepdqrsq
     1021 ensdymfrgd ggpakvtagt gswccrrggt eqptpehlrd gccqglqtrd eiladsadks
     1081 dvknegpqsp rsgsvpsatt klqdhldklk nnvrtevpdc ncfpadkcpp epgsyythlg
     1141 aaaslpdlrn dlerrtglkg nairfekviy tgkegkttqg cpmakwilrr sgldekilti
     1201 vkhrqghkcp tawivvamva wegvpthead riysllshkl nrfglpttrr cgtneprtca
     1261 cqgldpdtcg asfsfgcsws myyngckyar sktvrkfrls vrseeqevee rmhvlatlls
     1321 plylslapea fnnqtqfere asecrlgfkp grpfsgvtac idfcahshrd lhnmnngctv
     1381 vvtltkhrtl skpedeqlhv lplyimdttd eygskegqde kvrdgsvevl tkypcevrvr
     1441 svplqpcrrh gkkrkedepd tvsnkkdgsk ntgekmneqg rvpghqeaak pqmrdtqlsl
     1501 emasmfegmd aqlqssqvss tvldspvsmy qgwgyqneqq wgrngwldqr knnwlnpwge
     1561 ysfggldrea kvdpdstsld dtrprsavsq ddhrpgsrnl pnstidsprn cyphsprthp
     1621 isprsshast pgdvcysmsp rgcpptphdq kmasprssgy pdtgythvpt srnynvydnr
     1681 qgsaspktgc tnfpphldqr salgrnhpqh inalrqsceq skfpfskpah dssqqkyhmt
     1741 qnyleaqksq teqsftgriq ghypshpvqs vrnhpyqnqh dsnmdvypgl savspcmdna
     1801 shkafgatnp dlllhssshl ppnnqqngaq nyspvdqrry ktqkvpeqrm pgvnhmspap
     1861 sdqaggwnml gswypdqskp ynqqimyndq iaserthqqp vdhqktlnwn driphpdqpk
     1921 ppcweappdp spfrvpkgrp psrtassqnp nadntyqnss nrtflkpqep mrtavyadsl
     1981 sngvqsigtm hqsnqrkpew peenkikegf hdnrqnvanp nsncfpwpee mkqvqdfhpv
     2041 palghphasf pqygpyptyp vfekpysnsw egynyhqtpy hpqtpeyppq fyqqpkreaf
     2101 vpspqypyqg vppyqglnpg warwetprwd iygpppyfpv lpepppkaep lgevadysdn
     2161 eecfkdsqmg gvaialghgs vlfecakhem hsttalkkpn rlnptrislv fyqhrnlnrp
     2221 rhgwdeweek mrlrklgvts ttgttaitts sttsqslstp stptspasai ineksiplpy
     2281 ippvpssqfm mrsptyttmt wttlfpmhpc mitgpyqegg aig
//
LOCUS       XP_034183039             341 aa            linear   INV 03-SEP-2025
DEFINITION  spatzle 5 Toll-1 receptor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183039
VERSION     XP_034183039.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327148.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183039.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..341
                     /product="spatzle 5 Toll-1 receptor isoform X1"
                     /calculated_mol_wt=38620
     Region          <108..179
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          241..335
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..341
                     /gene="spz5"
                     /coded_by="XM_034327148.2:655..1680"
                     /db_xref="GeneID:117605619"
ORIGIN      
        1 mlgsdmrpff illiitslan shnepcleyg cpgrpqyepf vpappghtpn cakpgqtfce
       61 spdhyprqli kflvekcsfd fssvlrdesk ddfniyrsvp dyaegydytp hggpqlhsqp
      121 lpflppqypl rgqpaliygs psndthqngy rystpaqrnp flvdgtpsvk yhpvgqrtpe
      181 rhfysqsqsl nsfgqgqtww anrytrnska aprsvyenpl lkyskpskmr nkrqsnldsi
      241 picptvpqyi tpraalnnqg nwmyvvnlqd qnqkysqivk sekcststcn alcsvpagyt
      301 skcqqqyvqk rlvalegsgn qlytdvfwfp hgcscqimln y
//
LOCUS       XP_034183040             333 aa            linear   INV 03-SEP-2025
DEFINITION  spatzle 5 Toll-1 receptor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183040
VERSION     XP_034183040.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327149.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183040.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..333
                     /product="spatzle 5 Toll-1 receptor isoform X2"
                     /calculated_mol_wt=37708
     Region          <100..171
                     /region_name="PABP-1234"
                     /note="polyadenylate binding protein, human types 1, 2, 3,
                     4 family; TIGR01628"
                     /db_xref="CDD:130689"
     Region          233..327
                     /region_name="Spaetzle"
                     /note="pfam16077"
                     /db_xref="CDD:465007"
     CDS             1..333
                     /gene="spz5"
                     /coded_by="XM_034327149.2:337..1338"
                     /db_xref="GeneID:117605619"
ORIGIN      
        1 mnnnecmtsl anshnepcle ygcpgrpqye pfvpappght pncakpgqtf cespdhyprq
       61 likflvekcs fdfssvlrde skddfniyrs vpdyaegydy tphggpqlhs qplpflppqy
      121 plrgqpaliy gspsndthqn gyrystpaqr npflvdgtps vkyhpvgqrt perhfysqsq
      181 slnsfgqgqt wwanrytrns kaaprsvyen pllkyskpsk mrnkrqsnld sipicptvpq
      241 yitpraalnn qgnwmyvvnl qdqnqkysqi vksekcstst cnalcsvpag ytskcqqqyv
      301 qkrlvalegs gnqlytdvfw fphgcscqim lny
//
LOCUS       XP_034186899            1088 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607377 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186899
VERSION     XP_034186899.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331008.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1088
                     /product="uncharacterized protein LOC117607377 isoform X1"
                     /calculated_mol_wt=122000
     Region          <227..>493
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <712..777
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1005..>1051
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1088
                     /gene="LOC117607377"
                     /coded_by="XM_034331008.2:135..3401"
                     /db_xref="GeneID:117607377"
ORIGIN      
        1 msehdetild edlgdeeynl gndeeealla ddyeldrqns ykgeeetddv ldlgvtdgld
       61 dldgedenie fsrsktdrrd dndfyedgdq leiqskyyeq delneykseq trqhdehsai
      121 dsvntssnig kgdlreklqk nakiysgngq gleddeceea kerrnrfqne rtivspkmnn
      181 nipetlenvv taersfrgrg rgrstrgsrg grfniqntgn fnprfnnars tnfdgqppvc
      241 rpplletrpp fllgvpsnmq nqqiiyqqqn sqvqpfqhys tngplppgpt hfldnrpqln
      301 pnqfqgqvgg prvigpridy ghrgglagpg pgasynhppn qppyvqiqpg pfqnsgvlmq
      361 dnqqrlmqnn qgpvlqgnag gsllgnlnsv vppgtsapll qagnqplpmq gfprqtssqg
      421 pplinhpnvq ipnqspfenr ppfqeppfen rpvydsrsgy sdqvptsqfn tntlpvpqqs
      481 tsnvpnvplp pghkilinph frgsvqstnd arliwdstqq qqqqpslssq ipgsqfsqsa
      541 isyqnqssyn qtqqgsshyq qnyqqnksdd pyayfsdvwq enkpqksqss apskqypsds
      601 nypressyey sskyksdqwd qkdsyqddhr gvhqsyrdrd lssktrsdhv srsrapssns
      661 yrdnydqnhv qksnnrsanp sirprlpqkr ipdssdrplr dlspkrikps nrnlqevrtv
      721 dtlntvgnek kdedndpemq eyrkkmeeqk rlrekilrek enrrkmaame kqneeakids
      781 ttpgentqdt kststlmgvv kdasvnkght gvgrvrgrpm ntqtteamek pscirivrtv
      841 qtvqsndsvd sakdsnaght inqagehakv lnsqtgarri viqkslsntq kvatsiqktv
      901 snlqkgqppv qkvintmqgg vqkvgqsqsd vlkkitntgq riggnpqriv kqkspgnlqk
      961 nvinvqevtn anaqkvfvnt hnnqrvvlqk astqqkkvpe iktntvrven laastseaqi
     1021 rrmcqsigti esiqmgersa tivfktqsaa mvfhkkyqrk mldlslitvr lvsqssgikt
     1081 ttgiakvs
//
LOCUS       XP_034186900            1087 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607377 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186900
VERSION     XP_034186900.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331009.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1087
                     /product="uncharacterized protein LOC117607377 isoform X2"
                     /calculated_mol_wt=122002
     Region          <227..>493
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     Region          <712..777
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     Region          1004..>1050
                     /region_name="RRM_SF"
                     /note="RNA recognition motif (RRM) superfamily; cl17169"
                     /db_xref="CDD:473069"
     CDS             1..1087
                     /gene="LOC117607377"
                     /coded_by="XM_034331009.2:135..3398"
                     /db_xref="GeneID:117607377"
ORIGIN      
        1 msehdetild edlgdeeynl gndeeealla ddyeldrqns ykgeeetddv ldlgvtdgld
       61 dldgedenie fsrsktdrrd dndfyedgdq leiqskyyeq delneykseq trqhdehsai
      121 dsvntssnig kgdlreklqk nakiysgngq gleddeceea kerrnrfqne rtivspkmnn
      181 nipetlenvv taersfrgrg rgrstrgsrg grfniqntgn fnprfnnars tnfdgqppvc
      241 rpplletrpp fllgvpsnmq nqqiiyqqqn sqvqpfqhys tngplppgpt hfldnrpqln
      301 pnqfqgqvgg prvigpridy ghrgglagpg pgasynhppn qppyvqiqpg pfqnsgvlmq
      361 dnqqrlmqnn qgpvlqgnag gsllgnlnsv vppgtsapll qagnqplpmq gfprqtssqg
      421 pplinhpnvq ipnqspfenr ppfqeppfen rpvydsrsgy sdqvptsqfn tntlpvpqqs
      481 tsnvpnvplp pghkilinph frgsvqstnd arliwdstqq qqqqpslssq ipgsqfsqsa
      541 isyqnqssyn qtqqgsshyq qnyqqnksdd pyayfsdvwq enkpqksqss apskqypsds
      601 nypressyey sskyksdqwd qkdsyqddhr gvhqsyrdrd lssktrsdhv srsrapssns
      661 yrdnydqnhv qksnnrsanp sirprlpqkr ipdssdrplr dlspkrikps nrnlqevrtv
      721 dtlntvgnek kdedndpemq eyrkkmeeqk rlrekilrek enrrkmaame kqneeakids
      781 ttrentqdtk ststlmgvvk dasvnkghtg vgrvrgrpmn tqtteamekp scirivrtvq
      841 tvqsndsvds akdsnaghti nqagehakvl nsqtgarriv iqkslsntqk vatsiqktvs
      901 nlqkgqppvq kvintmqggv qkvgqsqsdv lkkitntgqr iggnpqrivk qkspgnlqkn
      961 vinvqevtna naqkvfvnth nnqrvvlqka stqqkkvpei ktntvrvenl aastseaqir
     1021 rmcqsigtie siqmgersat ivfktqsaam vfhkkyqrkm ldlslitvrl vsqssgiktt
     1081 tgiakvs
//
LOCUS       XP_034186901             704 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607378 [Osmia lignaria lignaria].
ACCESSION   XP_034186901
VERSION     XP_034186901.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331010.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..704
                     /product="uncharacterized protein LOC117607378"
                     /calculated_mol_wt=77274
     Region          552..681
                     /region_name="PEHE"
                     /note="PEHE domain; pfam15275"
                     /db_xref="CDD:464607"
     CDS             1..704
                     /gene="LOC117607378"
                     /coded_by="XM_034331010.2:319..2433"
                     /db_xref="GeneID:117607378"
ORIGIN      
        1 mssgsasklv ngenkkenvh gyeyenkket vsgaagstgs sivltgdtsn attivtvpgt
       61 gttvpsitgn scsgsgcgng gsvcftgsgn gdsdsnsgvc vtgltvggvv gvefcdvnsd
      121 lktadnleev hdqrdvndvt efgetvfqdp ytgtcietpv dslatttttt tatttttiti
      181 iehddrtstt vsapcyeedq eedneedddn mvaamnehls ttsatsgirs stvstttttg
      241 nsvvnqnnsc srneyqnvqe dskpaagils fpcdfdhmya smtdggapaa atatsalgvs
      301 plrgneprqn dltevkhlke llllhldliq qqseqivtkd kllaalrqen etlklrlerm
      361 drrvnlqklr sensensitp ehigtcsppt vnsvnvpsts elhrnvqsen nnsqynesfk
      421 irlntsggit tvkqepnenq nkhetkietg ndgrldweek kkrrtdpipl stgskrkrgv
      481 scsstisndt sstghdktlt qetgrksdrk gktitkkesf ltteehyyta vgdpsytlsl
      541 kepnpvesdt slevpnwrvk iytscytmeg tenlddeifn krhlklende rrrkrwdvqr
      601 ireqrhiekl kqrqerqnhq atcyntnhtg pcpstveeet vsslwpdveq iqslqvdphl
      661 pvtafgapip sftpsefslp wlnmtrsscr rpkrstgrrk strr
//
LOCUS       XP_034186903             570 aa            linear   INV 03-SEP-2025
DEFINITION  heat shock protein 60A [Osmia lignaria lignaria].
ACCESSION   XP_034186903
VERSION     XP_034186903.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331012.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="heat shock protein 60A"
                     /calculated_mol_wt=60254
     Region          23..541
                     /region_name="GroEL"
                     /note="GroEL_like type I chaperonin. Chaperonins are
                     involved in productive folding of proteins. They share a
                     common general morphology, a double toroid of 2 stacked
                     rings, each composed of 7-9 subunits. The symmetry of type
                     I is seven-fold and they are found...; cd03344"
                     /db_xref="CDD:239460"
     Site            order(23,27,44,55..58,60,65..66,68,78,80,88,92,95,216,248,
                     276,404,406,479,531,534..540)
                     /site_type="other"
                     /note="ring oligomerisation interface [polypeptide
                     binding]"
                     /db_xref="CDD:239460"
     Site            order(50..52,106,110,169,418,435,474,511,513)
                     /site_type="other"
                     /note="ATP/Mg binding site [chemical binding]"
                     /db_xref="CDD:239460"
     Site            order(128,454,472,481,483..484,487)
                     /site_type="active"
                     /note="stacking interactions [active]"
                     /db_xref="CDD:239460"
     Site            order(160,205,212,395,429..430)
                     /site_type="other"
                     /note="hinge regions"
                     /db_xref="CDD:239460"
     CDS             1..570
                     /gene="LOC117607379"
                     /coded_by="XM_034331012.2:160..1872"
                     /db_xref="GeneID:117607379"
ORIGIN      
        1 mhrlptvlrs talrqlqars yakdvrfgse vralmlqgvd iladavavtm gpkgrnvile
       61 qswgspkitk dgvtvakgve lkdkfqniga klvqdvannt neeagdgttt atvlaraiak
      121 dgfekiskga npveirrgvm lavdkvkdel kalskpvttp eeiaqvatis angdkaignl
      181 isdamkkvgk egvitvkdgk tlydelevie gmkfdrgyis pyfinsskga kvefqdalll
      241 ysekkissvq siipalelan sqrkplviia edidgealst lvvnrlkigl qvaavkapgf
      301 gdnrkatlqd maiatggivf gddanlvkie nvqlsdlgev gevvitkddt lllkgkgkkn
      361 diehradvir dqianttsdy ekeklqerla rlasgvavlr vggssevevn ekkdrvhdal
      421 natraaveeg ivpgggtall rcipalrsmk alnsdqetgi kivasalrmp clqiaqnagv
      481 dasvvvakvs esnlgydaln deyvdmiekg iidptkvvrt altdaagvas llttaeavva
      541 elpkeepqmp mggggmggmg gmggmggmgm
//
LOCUS       XP_034186904             533 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 2, facilitated glucose transporter member 1
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186904
VERSION     XP_034186904.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331013.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 1 isoform X1"
                     /calculated_mol_wt=58117
     Region          81..518
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(84..85,88..89,92,138,191..192,194..196,199,219,
                     222..223,226,337,340..341,344..346,349,377,381,439..440,
                     444,448,464,467..468,471..472,475)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..533
                     /gene="LOC117607380"
                     /coded_by="XM_034331013.2:223..1824"
                     /db_xref="GeneID:117607380"
ORIGIN      
        1 mdrddedfge gfiaeshplr pthlsvplsg rpdsrapsis ssvsdldvpi ytrettipls
       61 aqrglngrla faiaaaalgs sfqhgyntgv vnapqqlied wisnlkmnrt geptkqsevt
      121 miwsiavsif cvggmiggsl vgtvadrfgr kgglllnnil vlltvifegc aktaksyemi
      181 iigrfligin aglnaglapm ylaeispihl rgavgtvyql vitisilvsq ilgleqilgt
      241 adqwplllcl tivpaifqvf tlpmcpespk ylllsrgkdm eaqralswlr gtlevhdeme
      301 emraeyesvk lvpkvtlkel fvnpalripl miaimimfaq qlsginavmf fstkifmmaq
      361 ldknaaqnat mgvgamnvvm tfislilver agrktlllig fggmfidtal laiclvfaet
      421 sstaayfsiv lvimfvvlfa tgpgsipwfl vselfnqsar paatsvaiav nwtanfivsi
      481 gflplqealg ayvfimfavi qaffvffiyk kvpetknktm eeissmfrqi syq
//
LOCUS       XP_034186905             533 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 2, facilitated glucose transporter member 1
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186905
VERSION     XP_034186905.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331014.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 1 isoform X1"
                     /calculated_mol_wt=58117
     Region          81..518
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(84..85,88..89,92,138,191..192,194..196,199,219,
                     222..223,226,337,340..341,344..346,349,377,381,439..440,
                     444,448,464,467..468,471..472,475)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..533
                     /gene="LOC117607380"
                     /coded_by="XM_034331014.2:328..1929"
                     /db_xref="GeneID:117607380"
ORIGIN      
        1 mdrddedfge gfiaeshplr pthlsvplsg rpdsrapsis ssvsdldvpi ytrettipls
       61 aqrglngrla faiaaaalgs sfqhgyntgv vnapqqlied wisnlkmnrt geptkqsevt
      121 miwsiavsif cvggmiggsl vgtvadrfgr kgglllnnil vlltvifegc aktaksyemi
      181 iigrfligin aglnaglapm ylaeispihl rgavgtvyql vitisilvsq ilgleqilgt
      241 adqwplllcl tivpaifqvf tlpmcpespk ylllsrgkdm eaqralswlr gtlevhdeme
      301 emraeyesvk lvpkvtlkel fvnpalripl miaimimfaq qlsginavmf fstkifmmaq
      361 ldknaaqnat mgvgamnvvm tfislilver agrktlllig fggmfidtal laiclvfaet
      421 sstaayfsiv lvimfvvlfa tgpgsipwfl vselfnqsar paatsvaiav nwtanfivsi
      481 gflplqealg ayvfimfavi qaffvffiyk kvpetknktm eeissmfrqi syq
//
LOCUS       XP_034186906             533 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 2, facilitated glucose transporter member 1
            isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186906
VERSION     XP_034186906.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331015.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 1 isoform X1"
                     /calculated_mol_wt=58117
     Region          81..518
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(84..85,88..89,92,138,191..192,194..196,199,219,
                     222..223,226,337,340..341,344..346,349,377,381,439..440,
                     444,448,464,467..468,471..472,475)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..533
                     /gene="LOC117607380"
                     /coded_by="XM_034331015.2:96..1697"
                     /db_xref="GeneID:117607380"
ORIGIN      
        1 mdrddedfge gfiaeshplr pthlsvplsg rpdsrapsis ssvsdldvpi ytrettipls
       61 aqrglngrla faiaaaalgs sfqhgyntgv vnapqqlied wisnlkmnrt geptkqsevt
      121 miwsiavsif cvggmiggsl vgtvadrfgr kgglllnnil vlltvifegc aktaksyemi
      181 iigrfligin aglnaglapm ylaeispihl rgavgtvyql vitisilvsq ilgleqilgt
      241 adqwplllcl tivpaifqvf tlpmcpespk ylllsrgkdm eaqralswlr gtlevhdeme
      301 emraeyesvk lvpkvtlkel fvnpalripl miaimimfaq qlsginavmf fstkifmmaq
      361 ldknaaqnat mgvgamnvvm tfislilver agrktlllig fggmfidtal laiclvfaet
      421 sstaayfsiv lvimfvvlfa tgpgsipwfl vselfnqsar paatsvaiav nwtanfivsi
      481 gflplqealg ayvfimfavi qaffvffiyk kvpetknktm eeissmfrqi syq
//
LOCUS       XP_034186907             505 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 2, facilitated glucose transporter member 1
            isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186907
VERSION     XP_034186907.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331016.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..505
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 1 isoform X2"
                     /calculated_mol_wt=55007
     Region          53..490
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(56..57,60..61,64,110,163..164,166..168,171,191,
                     194..195,198,309,312..313,316..318,321,349,353,411..412,
                     416,420,436,439..440,443..444,447)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..505
                     /gene="LOC117607380"
                     /coded_by="XM_034331016.2:423..1940"
                     /db_xref="GeneID:117607380"
ORIGIN      
        1 matekdksnn eiqeqvttpt altpapksie gdkqlglngr lafaiaaaal gssfqhgynt
       61 gvvnapqqli edwisnlkmn rtgeptkqse vtmiwsiavs ifcvggmigg slvgtvadrf
      121 grkgglllnn ilvlltvife gcaktaksye miiigrflig inaglnagla pmylaeispi
      181 hlrgavgtvy qlvitisilv sqilgleqil gtadqwplll cltivpaifq vftlpmcpes
      241 pkylllsrgk dmeaqralsw lrgtlevhde meemraeyes vklvpkvtlk elfvnpalri
      301 plmiaimimf aqqlsginav mffstkifmm aqldknaaqn atmgvgamnv vmtfislilv
      361 eragrktlll igfggmfidt allaiclvfa etsstaayfs ivlvimfvvl fatgpgsipw
      421 flvselfnqs arpaatsvai avnwtanfiv sigflplqea lgayvfimfa viqaffvffi
      481 ykkvpetknk tmeeissmfr qisyq
//
LOCUS       XP_034186908             486 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 2, facilitated glucose transporter member 1
            isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186908
VERSION     XP_034186908.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331017.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..486
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 1 isoform X3"
                     /calculated_mol_wt=53063
     Region          34..471
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(37..38,41..42,45,91,144..145,147..149,152,172,
                     175..176,179,290,293..294,297..299,302,330,334,392..393,
                     397,401,417,420..421,424..425,428)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..486
                     /gene="LOC117607380"
                     /coded_by="XM_034331017.2:148..1608"
                     /db_xref="GeneID:117607380"
ORIGIN      
        1 mtlktttfkf pvlkksglng rlafaiaaaa lgssfqhgyn tgvvnapqql iedwisnlkm
       61 nrtgeptkqs evtmiwsiav sifcvggmig gslvgtvadr fgrkggllln nilvlltvif
      121 egcaktaksy emiiigrfli ginaglnagl apmylaeisp ihlrgavgtv yqlvitisil
      181 vsqilgleqi lgtadqwpll lcltivpaif qvftlpmcpe spkylllsrg kdmeaqrals
      241 wlrgtlevhd emeemraeye svklvpkvtl kelfvnpalr iplmiaimim faqqlsgina
      301 vmffstkifm maqldknaaq natmgvgamn vvmtfislil veragrktll ligfggmfid
      361 tallaiclvf aetsstaayf sivlvimfvv lfatgpgsip wflvselfnq sarpaatsva
      421 iavnwtanfi vsigflplqe algayvfimf aviqaffvff iykkvpetkn ktmeeissmf
      481 rqisyq
//
LOCUS       XP_034186909             477 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family 2, facilitated glucose transporter member 1
            isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_034186909
VERSION     XP_034186909.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331018.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..477
                     /product="solute carrier family 2, facilitated glucose
                     transporter member 1 isoform X4"
                     /calculated_mol_wt=52190
     Region          25..462
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(28..29,32..33,36,82,135..136,138..140,143,163,
                     166..167,170,281,284..285,288..290,293,321,325,383..384,
                     388,392,408,411..412,415..416,419)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:349949"
     CDS             1..477
                     /gene="LOC117607380"
                     /coded_by="XM_034331018.2:119..1552"
                     /db_xref="GeneID:117607380"
ORIGIN      
        1 mnkkwyqgln grlafaiaaa algssfqhgy ntgvvnapqq liedwisnlk mnrtgeptkq
       61 sevtmiwsia vsifcvggmi ggslvgtvad rfgrkgglll nnilvlltvi fegcaktaks
      121 yemiiigrfl iginaglnag lapmylaeis pihlrgavgt vyqlvitisi lvsqilgleq
      181 ilgtadqwpl llcltivpai fqvftlpmcp espkylllsr gkdmeaqral swlrgtlevh
      241 demeemraey esvklvpkvt lkelfvnpal riplmiaimi mfaqqlsgin avmffstkif
      301 mmaqldknaa qnatmgvgam nvvmtfisli lveragrktl lligfggmfi dtallaiclv
      361 faetsstaay fsivlvimfv vlfatgpgsi pwflvselfn qsarpaatsv aiavnwtanf
      421 ivsigflplq ealgayvfim faviqaffvf fiykkvpetk nktmeeissm frqisyq
//
LOCUS       XP_034186910             475 aa            linear   INV 03-SEP-2025
DEFINITION  putative ATP-dependent RNA helicase Dbp80 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186910
VERSION     XP_034186910.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331019.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..475
                     /product="putative ATP-dependent RNA helicase Dbp80"
                     /calculated_mol_wt=52956
     Region          89..472
                     /region_name="SrmB"
                     /note="Superfamily II DNA and RNA helicase [Replication,
                     recombination and repair]; COG0513"
                     /db_xref="CDD:440279"
     CDS             1..475
                     /gene="Dbp80"
                     /coded_by="XM_034331019.2:137..1564"
                     /db_xref="GeneID:117607381"
ORIGIN      
        1 masseldwgk yvdeqeklsa kvsslnldkp vkdsnastds ksddgtdeqi spaeksllqk
       61 iirrglmett kdleiqrkdp ssplysvktf ealhlkpall kgvyalgfna pskiqetalp
      121 tlladppqnm iaqsqsgtgk taafvlamls rvdttknypq vlclsptyel aiqtgevaac
      181 msafcseiki kyavrgeeis rgskitehii igtpgkvldw avkfkffsls kisvfvldea
      241 dvmiatqghq dqcirihkql prtcqmmffs atyepevmkf aeiivnnpli irllkeeesl
      301 dnikqyyvrc kdldekyaai tniygvitig qaiifchtrr tanwlsermt kdghavavls
      361 geltveqrit vldrfrsgle kvlittnvla rgidieqvti vvnfdlpmdq nrqadcetyl
      421 hrigrtgrfg ksgiainlid sphamqlckd iekhfgkkih yldaedadei ekiga
//
LOCUS       XP_034186912             426 aa            linear   INV 03-SEP-2025
DEFINITION  threonyl-carbamoyl synthesis 4 [Osmia lignaria lignaria].
ACCESSION   XP_034186912
VERSION     XP_034186912.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331021.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..426
                     /product="threonyl-carbamoyl synthesis 4"
                     /calculated_mol_wt=47459
     Region          44..377
                     /region_name="ASKHA_NBD_OSGEPL1_QRI7_euk"
                     /note="nucleotide-binding domain (NBD) of
                     O-sialoglycoprotein endopeptidase-like protein 1 (OSGEPL1)
                     and similar proteins from eukayotes; cd24134"
                     /db_xref="CDD:466984"
     CDS             1..426
                     /gene="Tcs4"
                     /coded_by="XM_034331021.2:1..1281"
                     /db_xref="GeneID:117607382"
ORIGIN      
        1 mtwtirlfhk cqqkhfintr aflhsykndv ervqnsqpdk paiilgvess cdetgcgivd
       61 stgtilgeai ssqqlnhlnf ggiiptiaqm lhrdnitkvc edalqsanlk lrdvdaiatt
      121 vkpgirpslg igtkfgkyla kignkpfipi hhmeahalta riqekvdfpy lvllisgghc
      181 llgivestnk fyllgttkdn apgevfdkia rrlklrnipe fntlsggqal eiaaskatdi
      241 nqfafspimt rlydcnfsfa giqstckryi qfeeekhnii admlipdvyn lcaafqlaai
      301 thvcqrtqra mkfiekmslf penkrtlvis ggvacnnlla kalsivcsel gykfirtppk
      361 lctdngimia wngvekwitg tdiitdpcei ekveiepkas fgenwiqrvq aanvkcewip
      421 ikrkly
//
LOCUS       XP_034186913             389 aa            linear   INV 03-SEP-2025
DEFINITION  GTP-binding protein 10 homolog [Osmia lignaria lignaria].
ACCESSION   XP_034186913
VERSION     XP_034186913.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331022.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..389
                     /product="GTP-binding protein 10 homolog"
                     /calculated_mol_wt=42967
     Region          23..307
                     /region_name="Obg"
                     /note="GTPase involved in cell partioning and DNA repair
                     [Cell cycle control, cell division, chromosome
                     partitioning, Replication, recombination, and repair];
                     COG0536"
                     /db_xref="CDD:440302"
     Region          <314..>362
                     /region_name="PRK10919"
                     /note="ATP-dependent DNA helicase Rep; Provisional"
                     /db_xref="CDD:182838"
     CDS             1..389
                     /gene="LOC117607383"
                     /coded_by="XM_034331022.2:187..1356"
                     /db_xref="GeneID:117607383"
ORIGIN      
        1 mvlltrvlgy ssklprkykr tgfidslriy vsagsggsgl hrygglggag gnvcvvaked
       61 ltlrtvknki envklkaesg gestkngiig tpgkdliiki pigvtvydds riklgelnse
      121 neklivakgg lggsentgyc glkgerrgii ldlkliadvg ligfpnagks tllnaiskak
      181 pkiadypftt ikpqigiiny ndyrqisvad lpgliegahv nkgmgfnflk hiertklllf
      241 vidiqgfqis vkhqyrscle tvlllnkeie lykpdlldkp amivinkmdt eganeiykdi
      301 kpklsnlsea slefdeamqs kkflqfddil ptalikkdad dvqrikekir yiidkyeeik
      361 asdqsnesne drlyeklkrq inrhaptll
//
LOCUS       XP_034186914             376 aa            linear   INV 03-SEP-2025
DEFINITION  actin, clone 403 [Osmia lignaria lignaria].
ACCESSION   XP_034186914
VERSION     XP_034186914.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331023.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="actin, clone 403"
                     /calculated_mol_wt=41666
     Region          7..371
                     /region_name="ASKHA_NBD_actin"
                     /note="nucleotide-binding domain (NBD) of actin and
                     similar proteins; cd10224"
                     /db_xref="CDD:466823"
     CDS             1..376
                     /gene="LOC117607384"
                     /coded_by="XM_034331023.2:466..1596"
                     /db_xref="GeneID:117607384"
ORIGIN      
        1 mcdddvaalv vdngsgmcka gfagddapra vfpsivgrpr yqgimvgmgq kdsyvgdeaq
       61 skrgvltvky piehgivtnw ddmekiwhht fynelriape ehpvllteap lnpkanrekm
      121 tqimfetfns pamyvaiqav lslyasgrtt givldsgdgv shtvpiyegy alphailrmd
      181 lagrdltdyl mkiltergys ftttaereiv rdikeklcyv aldfqqemat aagssaleks
      241 yelpdgqvit ignerfrcpe amfqpsflgm escgihetty nsimkcdvdi rkdlyansvl
      301 sggttmypgi adrmqkeita lapstmkiki iapperkysv wiggsilasl stfqqmwisk
      361 qeydesgpsi vhrkcf
//
LOCUS       XP_034191226            1904 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase II subunit RpII215 [Osmia lignaria lignaria].
ACCESSION   XP_034191226
VERSION     XP_034191226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335335.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1904
                     /product="RNA polymerase II subunit RpII215"
                     /calculated_mol_wt=209671
     Region          14..868
                     /region_name="RNAP_II_RPB1_N"
                     /note="Largest subunit (Rpb1) of eukaryotic RNA polymerase
                     II (RNAP II), N-terminal domain; cd02733"
                     /db_xref="CDD:259848"
     Site            order(21,25,28..29,76..78,80,91,242,244,246,251..252,310,
                     331,334..335,338,345,348,350..357,362..364,376,379..380,
                     449,451,453,457..458,460..461,472..473,475,478,487..490,
                     492,494,496,499..500,503,506..507,510..511,516,531..532,
                     535,672..673,761,766..767,772,775..776,785..786,816..817,
                     819..821,824..825,828..829,831..833,836..837,839..840,
                     853..854,857,861)
                     /site_type="other"
                     /note="RPB1 - RPB2 interface [polypeptide binding]"
                     /db_xref="CDD:259848"
     Site            order(21..33,36,39,78..103,237..256,294..300,303..311,
                     330..346,348..352)
                     /site_type="other"
                     /note="clamp"
                     /db_xref="CDD:259848"
     Site            order(39..43,74,254,256,262..267,269..271,273..274,277,
                     281,297,300..301,304..305,314..319,322..327,329,334,339,
                     410,413,417..420,422..424)
                     /site_type="other"
                     /note="RPB1-TFIIB interface [polypeptide binding]"
                     /db_xref="CDD:259848"
     Site            order(66,69,76,79)
                     /site_type="other"
                     /note="Zn-binding [ion binding]"
                     /db_xref="CDD:259848"
     Site            order(99..100,338,343,350,356,452..454,487,489,491,
                     850..851)
                     /site_type="active"
                     /note="active site region [active]"
                     /db_xref="CDD:259848"
     Region          890..1071
                     /region_name="RNA_pol_Rpb1_6"
                     /note="RNA polymerase Rpb1, domain 6; pfam04992"
                     /db_xref="CDD:461511"
     Region          1050..1469
                     /region_name="RNAP_II_Rpb1_C"
                     /note="Largest subunit (Rpb1) of Eukaryotic RNA polymerase
                     II (RNAP II), C-terminal domain; cd02584"
                     /db_xref="CDD:132720"
     Site            order(1069..1070,1074,1077,1461,1464..1467,1469)
                     /site_type="other"
                     /note="Rpb1 - Rpb6 interaction site [polypeptide binding]"
                     /db_xref="CDD:132720"
     Site            order(1074..1104,1106..1136,1142..1156,1293,1303..1312,
                     1314,1319..1380,1383..1402,1404..1418)
                     /site_type="active"
                     /note="cleft [active]"
                     /db_xref="CDD:132720"
     Site            order(1078,1081,1283,1287,1433,1443..1445,1451..1452,1454,
                     1456,1461)
                     /site_type="other"
                     /note="Rpb1 - Rpb2 interaction site [polypeptide binding]"
                     /db_xref="CDD:132720"
     Site            order(1123,1409,1426..1427,1430)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:132720"
     Site            order(1341,1347..1348,1350,1360..1366,1368,1388,
                     1399..1400,1402)
                     /site_type="other"
                     /note="Rpb1 - Rpb5 interaction site [polypeptide binding]"
                     /db_xref="CDD:132720"
     Site            1418..1459
                     /site_type="active"
                     /note="clamp [active]"
                     /db_xref="CDD:132720"
     Region          1499..1617
                     /region_name="CTD"
                     /note="Spt5 C-terminal nonapeptide repeat binding Spt4;
                     smart01104"
                     /db_xref="CDD:215026"
     Region          <1833..>1904
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1904
                     /gene="RpII215"
                     /coded_by="XM_034335335.2:72..5786"
                     /db_xref="GeneID:117609248"
ORIGIN      
        1 matsdskapl rtvkrvqfgi lspdeirrms vtdggirfpe tmeggrpklg glmdprqgvi
       61 drnsrcqtca gnmtecpghf ghidlakpvf hvgfitktik ilrcvcfycs kllvsphnpk
      121 ikeivmktkg qprkrltfvy dlcksknice ggdemdinke nqdqqnadrk pghggcgryq
      181 pnlqrsgldv taewkhvned sqekkialta eraweilkhi tdeesfilgm dpkfarpdwm
      241 vvtvlpvppl svrpavimyg saknqddlth kladiikann elirneqaga aahvisenik
      301 mlqfhvatlv dndmpgmpra mqksgkplka ikarlkgkeg rirgnlmgkr vdfsartvit
      361 pdpnlridqv gvprsiaqnl tfpeivtpfn idkmqelvrr gnsqypgaky ivrdngerid
      421 lrfhpkpsdl hlqcgyrver hirdgdlvif nrqptlhkms mmghrvkvlp wstfrmnlsc
      481 tspynadfdg demnlhvpqs metraeveni hvtprqiitp qankpvmgiv qdtltavrkm
      541 tkrdvfieke qmmnilmflp swdgkmpqpc ilkpkplwtg kqifsliipg nvnmirthst
      601 hpdeeddgpy kwispgdtkv mvehgelvmg ilckktlgts agsllhicml elghevcgrf
      661 ygniqtvinn wllleghsig igdtiadpqt yleiqkaikk akedvieviq kahnmelept
      721 pgntlrqtfe nqvnrilnda rdktggsakk slteynnlka mvvsgskgsn inisqviacv
      781 gqqnvegkri pfgfrkrtlp hfikddygpe srgfvensyl agltpsefyf hamggregli
      841 dtavktaetg yiqrrlikam esvmvhydgt vrnsvgqliq lrygedglcg etvefqnlpt
      901 iklsnkafek kfkfdptner ylrrifnedi vremmgsgev iselereweq lnkdrailre
      961 ifpsgeskvv lpcnlqrmiw nvqkifhink raptdlspmr viqgvkdlld kciivagddr
     1021 lskqanenat llfqclvrst lctkcvseef rlsseafewl igeietrfqq aqvspgemvg
     1081 alaaqslgep atqmtlntfh fagvssknvt lgvprlkeii niskkpkaps ltvfltgaaa
     1141 rdaekaknvl crlehttlrk vtantaiyyd pdpqntviae dqefvnvyye mpdfdptkis
     1201 pwllrieldr krmtdkkltm eqiaekinag fgddlncifn ddnaeklvlr irimnsddnk
     1261 fqdteeetvd kmeddmflrc ieanmlsdmt lqgieaigkv ymhlpqtdsk krivitetge
     1321 fkaiaewlle tdgtslmkvl serdvdpvrt fsndiceifq vlgieavrks vekemnavlq
     1381 fyglyvnyrh lallcdvmta kghlmaitrh ginrqdtgal mrcsfeetvd vlldaashae
     1441 vdpmrgvsen iimgqlprig tgcfdlllda ekckagieip mavgagvmgt agmffgsvat
     1501 psmspqmtpw mgatpgygas smspalgsgm tpggacfsps gasdasglsp aysayspqpg
     1561 spgspgpsms pypmspagga spsysptspa ylptspsitp sspnysptsp tysptspnys
     1621 ptspsyspts psysptspsy sptspsyspt spsysptsps ysptspsysp tspsysptsp
     1681 sysptspsys ptspsyspts psysptspsy sptspsyspt spsysptsps yspsspnytp
     1741 aspsysptsp syspsspqys paspsyspss pkysptspsy sptspsfagt spqytpaspt
     1801 ysptsptysp tspsyspssp qhtasgstry spsspnyspt sptysptspq yspsstkysp
     1861 tsptytptsp sysptsptys ppvpgyspts ptyspaspay etdd
//
LOCUS       XP_034191227            1904 aa            linear   INV 03-SEP-2025
DEFINITION  RNA polymerase II subunit RpII215 [Osmia lignaria lignaria].
ACCESSION   XP_034191227
VERSION     XP_034191227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335336.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1904
                     /product="RNA polymerase II subunit RpII215"
                     /calculated_mol_wt=209671
     Region          14..868
                     /region_name="RNAP_II_RPB1_N"
                     /note="Largest subunit (Rpb1) of eukaryotic RNA polymerase
                     II (RNAP II), N-terminal domain; cd02733"
                     /db_xref="CDD:259848"
     Site            order(21,25,28..29,76..78,80,91,242,244,246,251..252,310,
                     331,334..335,338,345,348,350..357,362..364,376,379..380,
                     449,451,453,457..458,460..461,472..473,475,478,487..490,
                     492,494,496,499..500,503,506..507,510..511,516,531..532,
                     535,672..673,761,766..767,772,775..776,785..786,816..817,
                     819..821,824..825,828..829,831..833,836..837,839..840,
                     853..854,857,861)
                     /site_type="other"
                     /note="RPB1 - RPB2 interface [polypeptide binding]"
                     /db_xref="CDD:259848"
     Site            order(21..33,36,39,78..103,237..256,294..300,303..311,
                     330..346,348..352)
                     /site_type="other"
                     /note="clamp"
                     /db_xref="CDD:259848"
     Site            order(39..43,74,254,256,262..267,269..271,273..274,277,
                     281,297,300..301,304..305,314..319,322..327,329,334,339,
                     410,413,417..420,422..424)
                     /site_type="other"
                     /note="RPB1-TFIIB interface [polypeptide binding]"
                     /db_xref="CDD:259848"
     Site            order(66,69,76,79)
                     /site_type="other"
                     /note="Zn-binding [ion binding]"
                     /db_xref="CDD:259848"
     Site            order(99..100,338,343,350,356,452..454,487,489,491,
                     850..851)
                     /site_type="active"
                     /note="active site region [active]"
                     /db_xref="CDD:259848"
     Region          890..1071
                     /region_name="RNA_pol_Rpb1_6"
                     /note="RNA polymerase Rpb1, domain 6; pfam04992"
                     /db_xref="CDD:461511"
     Region          1050..1469
                     /region_name="RNAP_II_Rpb1_C"
                     /note="Largest subunit (Rpb1) of Eukaryotic RNA polymerase
                     II (RNAP II), C-terminal domain; cd02584"
                     /db_xref="CDD:132720"
     Site            order(1069..1070,1074,1077,1461,1464..1467,1469)
                     /site_type="other"
                     /note="Rpb1 - Rpb6 interaction site [polypeptide binding]"
                     /db_xref="CDD:132720"
     Site            order(1074..1104,1106..1136,1142..1156,1293,1303..1312,
                     1314,1319..1380,1383..1402,1404..1418)
                     /site_type="active"
                     /note="cleft [active]"
                     /db_xref="CDD:132720"
     Site            order(1078,1081,1283,1287,1433,1443..1445,1451..1452,1454,
                     1456,1461)
                     /site_type="other"
                     /note="Rpb1 - Rpb2 interaction site [polypeptide binding]"
                     /db_xref="CDD:132720"
     Site            order(1123,1409,1426..1427,1430)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:132720"
     Site            order(1341,1347..1348,1350,1360..1366,1368,1388,
                     1399..1400,1402)
                     /site_type="other"
                     /note="Rpb1 - Rpb5 interaction site [polypeptide binding]"
                     /db_xref="CDD:132720"
     Site            1418..1459
                     /site_type="active"
                     /note="clamp [active]"
                     /db_xref="CDD:132720"
     Region          1499..1617
                     /region_name="CTD"
                     /note="Spt5 C-terminal nonapeptide repeat binding Spt4;
                     smart01104"
                     /db_xref="CDD:215026"
     Region          <1833..>1904
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     CDS             1..1904
                     /gene="RpII215"
                     /coded_by="XM_034335336.2:72..5786"
                     /db_xref="GeneID:117609248"
ORIGIN      
        1 matsdskapl rtvkrvqfgi lspdeirrms vtdggirfpe tmeggrpklg glmdprqgvi
       61 drnsrcqtca gnmtecpghf ghidlakpvf hvgfitktik ilrcvcfycs kllvsphnpk
      121 ikeivmktkg qprkrltfvy dlcksknice ggdemdinke nqdqqnadrk pghggcgryq
      181 pnlqrsgldv taewkhvned sqekkialta eraweilkhi tdeesfilgm dpkfarpdwm
      241 vvtvlpvppl svrpavimyg saknqddlth kladiikann elirneqaga aahvisenik
      301 mlqfhvatlv dndmpgmpra mqksgkplka ikarlkgkeg rirgnlmgkr vdfsartvit
      361 pdpnlridqv gvprsiaqnl tfpeivtpfn idkmqelvrr gnsqypgaky ivrdngerid
      421 lrfhpkpsdl hlqcgyrver hirdgdlvif nrqptlhkms mmghrvkvlp wstfrmnlsc
      481 tspynadfdg demnlhvpqs metraeveni hvtprqiitp qankpvmgiv qdtltavrkm
      541 tkrdvfieke qmmnilmflp swdgkmpqpc ilkpkplwtg kqifsliipg nvnmirthst
      601 hpdeeddgpy kwispgdtkv mvehgelvmg ilckktlgts agsllhicml elghevcgrf
      661 ygniqtvinn wllleghsig igdtiadpqt yleiqkaikk akedvieviq kahnmelept
      721 pgntlrqtfe nqvnrilnda rdktggsakk slteynnlka mvvsgskgsn inisqviacv
      781 gqqnvegkri pfgfrkrtlp hfikddygpe srgfvensyl agltpsefyf hamggregli
      841 dtavktaetg yiqrrlikam esvmvhydgt vrnsvgqliq lrygedglcg etvefqnlpt
      901 iklsnkafek kfkfdptner ylrrifnedi vremmgsgev iselereweq lnkdrailre
      961 ifpsgeskvv lpcnlqrmiw nvqkifhink raptdlspmr viqgvkdlld kciivagddr
     1021 lskqanenat llfqclvrst lctkcvseef rlsseafewl igeietrfqq aqvspgemvg
     1081 alaaqslgep atqmtlntfh fagvssknvt lgvprlkeii niskkpkaps ltvfltgaaa
     1141 rdaekaknvl crlehttlrk vtantaiyyd pdpqntviae dqefvnvyye mpdfdptkis
     1201 pwllrieldr krmtdkkltm eqiaekinag fgddlncifn ddnaeklvlr irimnsddnk
     1261 fqdteeetvd kmeddmflrc ieanmlsdmt lqgieaigkv ymhlpqtdsk krivitetge
     1321 fkaiaewlle tdgtslmkvl serdvdpvrt fsndiceifq vlgieavrks vekemnavlq
     1381 fyglyvnyrh lallcdvmta kghlmaitrh ginrqdtgal mrcsfeetvd vlldaashae
     1441 vdpmrgvsen iimgqlprig tgcfdlllda ekckagieip mavgagvmgt agmffgsvat
     1501 psmspqmtpw mgatpgygas smspalgsgm tpggacfsps gasdasglsp aysayspqpg
     1561 spgspgpsms pypmspagga spsysptspa ylptspsitp sspnysptsp tysptspnys
     1621 ptspsyspts psysptspsy sptspsyspt spsysptsps ysptspsysp tspsysptsp
     1681 sysptspsys ptspsyspts psysptspsy sptspsyspt spsysptsps yspsspnytp
     1741 aspsysptsp syspsspqys paspsyspss pkysptspsy sptspsfagt spqytpaspt
     1801 ysptsptysp tspsyspssp qhtasgstry spsspnyspt sptysptspq yspsstkysp
     1861 tsptytptsp sysptsptys ppvpgyspts ptyspaspay etdd
//
LOCUS       XP_034191228             586 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane 9 superfamily protein member 3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191228
VERSION     XP_034191228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335337.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..586
                     /product="transmembrane 9 superfamily protein member 3"
                     /calculated_mol_wt=67562
     Region          52..523
                     /region_name="EMP70"
                     /note="Endomembrane protein 70; pfam02990"
                     /db_xref="CDD:460774"
     CDS             1..586
                     /gene="TM9SF3"
                     /coded_by="XM_034335337.2:210..1970"
                     /db_xref="GeneID:117609249"
ORIGIN      
        1 mnmcrvrllf yflafsllpf vrsdehthiy edndevvlwm stvgpyhnrq etysyyslpf
       61 cmgtkdvinh yhetfsealq gielkfsgle ieykadvakv eycqikltee sekafiyavk
      121 nqywykmymd dlpiwgvvge peenngvvsy yiwthkkfdi gyngkqivdv nltsenkvkl
      181 vqgaaiffsy evnwkksnvk fedrfdkyld pnffqhrihw fsifnsfmmv iflvglvsmi
      241 lmrtlrkdya rysrdeemdd merdlgdeyg wkqvhgdvfr pashamlfsa ligagyqvtv
      301 vvlsviifai lgelytergs mlstaifvya atspingyag gglyarmggr iwikqmiasa
      361 fmlplivcgt affinfiamy yhasraipfg smvavtcici fvilpltlvg tilgrnlagt
      421 pdapcrvnav prpipekkwf meplviimlg gilpfgsifi emyfiftsfw aykiyyvygf
      481 mllvfvilmi vtvcvtivct yfllnaedyr wqwtsflaaa stagyvyiys fyyfffktkm
      541 yglfqtafyf gymalfslal gimcgtvgyv gtnafvrkiy stvkid
//
LOCUS       XP_034191229             551 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family member mahogany isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191229
VERSION     XP_034191229.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..551
                     /product="solute carrier family member mahogany isoform
                     X1"
                     /calculated_mol_wt=61522
     Region          36..386
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..551
                     /gene="mah"
                     /coded_by="XM_034335338.2:4..1659"
                     /db_xref="GeneID:117609250"
ORIGIN      
        1 mnnerlpllf kdsansdlsl lfatlcvidi fgvfpiialp rsivqcglyg iplvlvvfal
       61 qiytavllgk swtiattldp qisrknrhpl aavteltlgp rartlvtiil dlavfgcsip
      121 nllvasqnlq lfglkvssqr fdlsfcywll ivglllcpim wlgsprdmki lasfsstmvi
      181 ltavliwwci itddrelntt pipasptwdk lisgygmlaf qfdvhptlmt vqvdmrrpqd
      241 inkaviisfl vsgslfvvtt slaiwkyggs taanvlqmvp gnimmsaail faavqlclss
      301 alghsalfqd ledqcniqrt fgwkrcairs avvflgvavg esvprfdivm aliggsltgp
      361 lvfvlpplmy skaialkirs mrpltpeiys nserrrgneg mstdprihsr siyygvlgvp
      421 naeyhrysyf yyddleeefe eivdyddtls saktsegllm trrshdhepi fidanrpfkv
      481 crrttvsees vpkvkmncnf rrwnqwfsyl vvlfgivvti sstyinvknt iryvqftppc
      541 ivnvtvvqns v
//
LOCUS       XP_034191230             533 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family member mahogany isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191230
VERSION     XP_034191230.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..533
                     /product="solute carrier family member mahogany isoform
                     X2"
                     /calculated_mol_wt=59488
     Region          36..386
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..533
                     /gene="mah"
                     /coded_by="XM_034335339.2:4..1605"
                     /db_xref="GeneID:117609250"
ORIGIN      
        1 mnnerlpllf kdsansdlsl lfatlcvidi fgvfpiialp rsivqcglyg iplvlvvfal
       61 qiytavllgk swtiattldp qisrknrhpl aavteltlgp rartlvtiil dlavfgcsip
      121 nllvasqnlq lfglkvssqr fdlsfcywll ivglllcpim wlgsprdmki lasfsstmvi
      181 ltavliwwci itddrelntt pipasptwdk lisgygmlaf qfdvhptlmt vqvdmrrpqd
      241 inkaviisfl vsgslfvvtt slaiwkyggs taanvlqmvp gnimmsaail faavqlclss
      301 alghsalfqd ledqcniqrt fgwkrcairs avvflgvavg esvprfdivm aliggsltgp
      361 lvfvlpplmy skaialkirs mrpltpeiys nserrrgneg vpnaeyhrys yfyyddleee
      421 feeivdyddt lssaktsegl lmtrrshdhe pifidanrpf kvcrrttvse esvpkvkmnc
      481 nfrrwnqwfs ylvvlfgivv tisstyinvk ntiryvqftp pcivnvtvvq nsv
//
LOCUS       XP_034191231             492 aa            linear   INV 03-SEP-2025
DEFINITION  solute carrier family member mahogany isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191231
VERSION     XP_034191231.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335340.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..492
                     /product="solute carrier family member mahogany isoform
                     X3"
                     /calculated_mol_wt=54804
     Region          36..361
                     /region_name="SLC5-6-like_sbd"
                     /note="Solute carrier families 5 and 6-like; solute
                     binding domain; cl00456"
                     /db_xref="CDD:444915"
     CDS             1..492
                     /gene="mah"
                     /coded_by="XM_034335340.2:4..1482"
                     /db_xref="GeneID:117609250"
ORIGIN      
        1 mnnerlpllf kdsansdlsl lfatlcvidi fgvfpiialp rsivqcglyg iplvlvvfal
       61 qiytavllgk swtiattldp qisrknrhpl aavteltlgp rartlvtiil dlavfgcsip
      121 nllvasqnlq lfglkvssqr fdlsfcywll ivglllcpim wlgsprdmki lasfsstmvi
      181 ltavliwwci itddrelntt pipasptwdk lisgygmlaf qfdvhptlmt vqvdmrrpqd
      241 inkaviisfl vsgslfvvtt slaiwkyggs taanvlqmvp gnimmsaail faavqlclss
      301 alghsalfqd ledqcniqrt fgwkrcairs avvflgvavg esvprfdivm aliggsltgv
      361 pnaeyhrysy fyyddleeef eeivdyddtl ssaktsegll mtrrshdhep ifidanrpfk
      421 vcrrttvsee svpkvkmncn frrwnqwfsy lvvlfgivvt isstyinvkn tiryvqftpp
      481 civnvtvvqn sv
//
LOCUS       XP_034190893             530 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609108 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034190893
VERSION     XP_034190893.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335002.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..530
                     /product="uncharacterized protein LOC117609108 isoform X1"
                     /calculated_mol_wt=57472
     Region          107..438
                     /region_name="Zip"
                     /note="ZIP Zinc transporter; pfam02535"
                     /db_xref="CDD:396884"
     CDS             1..530
                     /gene="LOC117609108"
                     /coded_by="XM_034335002.2:1..1593"
                     /db_xref="GeneID:117609108"
ORIGIN      
        1 meepttstil liakigamig lgfgslilgm lplivgryri nhrqkrhrgi ssnsstctst
       61 svsnassstv fsgsatdsqv rfrctklspn nirnvqqfnp qlglqtslll cfgggvllft
      121 tflhlapevr vsverhqknd qlptlgtlsl sellfcagff lvylveeavh aaltgkpess
      181 eallyrtvsv rrcnnqpgas ttastgsttt mstttrstwr egndeleene nelnkqskhd
      241 kalpaifvls sptglssdeh spenvrypsd tnlnqyprtk qhehkhvpvt kntsiqgllt
      301 vlalsfhaif eglavgleps igsvvylaaa iathklvisf cvgmelyvag astrttlsyl
      361 tifsmvtpig iavglaldyf kndsgnlgpt ptilqgmaag tllyvvffev lareraneks
      421 gllqltaiiv gfmlmlglqi atahshshsh shshshghsh gndtdhhhed dhdheldsye
      481 ynhsnktseq iftdtidkvt esiaetvstv lsssmknptn tslrirngts
//
LOCUS       XP_034190894             507 aa            linear   INV 03-SEP-2025
DEFINITION  zinc transporter ZIP1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034190894
VERSION     XP_034190894.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335003.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..507
                     /product="zinc transporter ZIP1 isoform X2"
                     /calculated_mol_wt=54717
     Region          11..415
                     /region_name="Zip"
                     /note="ZIP Zinc transporter; pfam02535"
                     /db_xref="CDD:396884"
     CDS             1..507
                     /gene="LOC117609108"
                     /coded_by="XM_034335003.2:1..1524"
                     /db_xref="GeneID:117609108"
ORIGIN      
        1 meepttstil liakigamig lgfgslilgm lplivgryri nhrqkrhrgi ssnsstctst
       61 svsnassstv fsgsatdsqg lqtslllcfg ggvllfttfl hlapevrvsv erhqkndqlp
      121 tlgtlslsel lfcagfflvy lveeavhaal tgkpesseal lyrtvsvrrc nnqpgastta
      181 stgstttmst ttrstwregn deleenenel nkqskhdkal paifvlsspt glssdehspe
      241 nvrypsdtnl nqyprtkqhe hkhvpvtknt siqglltvla lsfhaifegl avglepsigs
      301 vvylaaaiat hklvisfcvg melyvagast rttlsyltif smvtpigiav glaldyfknd
      361 sgnlgptpti lqgmaagtll yvvffevlar eraneksgll qltaiivgfm lmlglqiata
      421 hshshshshs hshghshgnd tdhhheddhd heldsyeynh snktseqift dtidkvtesi
      481 aetvstvlss smknptntsl rirngts
//
LOCUS       XP_034190896             429 aa            linear   INV 03-SEP-2025
DEFINITION  zinc transporter ZIP1 isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034190896
VERSION     XP_034190896.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335005.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034190896.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..429
                     /product="zinc transporter ZIP1 isoform X3"
                     /calculated_mol_wt=47428
     Region          37..337
                     /region_name="Zip"
                     /note="ZIP Zinc transporter; pfam02535"
                     /db_xref="CDD:396884"
     CDS             1..429
                     /gene="LOC117609108"
                     /coded_by="XM_034335005.2:1..1290"
                     /db_xref="GeneID:117609108"
ORIGIN      
        1 mmpsednslm erlittdhfh ndeedhhhee edvydvlvak gvtmiilcvv stcmgilpmq
       61 makclkwkts dvgnprstkl vsillgfggg vlfcttflhl lpevkegveh lieekklpel
      121 sfslaetltc agfflmylve esvhthlrkk qsikddfskk dvnrstnelv enghshyngh
      181 shhlpvimde kddfvisslr gllivlglsv helfeglaig lessasfvwy mfaavaahkf
      241 viafcigvel iasntrtyls viyvctfavv splgigigml lvggesaaan gilpvllqgl
      301 asgtllyvvf feilqehrsg lkqylsilvg favmfvlqil tahshshshs hshshghshg
      361 ndtdhhhedd hdheldsyey nhsnktseqi ftdtidkvte siaetvstvl sssmknptnt
      421 slrirngts
//
LOCUS       XP_034191233             414 aa            linear   INV 03-SEP-2025
DEFINITION  pre-mRNA-splicing factor RBM22 [Osmia lignaria lignaria].
ACCESSION   XP_034191233
VERSION     XP_034191233.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335342.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..414
                     /product="pre-mRNA-splicing factor RBM22"
                     /calculated_mol_wt=46286
     Region          159..185
                     /region_name="ZnF_C3H1"
                     /note="zinc finger; smart00356"
                     /db_xref="CDD:214632"
     Region          231..304
                     /region_name="RRM_RBM22"
                     /note="RNA recognition motif (RRM) found in
                     Pre-mRNA-splicing factor RBM22 and similar proteins;
                     cd12224"
                     /db_xref="CDD:409671"
     CDS             1..414
                     /gene="LOC117609251"
                     /coded_by="XM_034335342.2:141..1385"
                     /db_xref="GeneID:117609251"
ORIGIN      
        1 matskttnty nrqnwedaef pilcqtclgd npyirmtkek ygkeckicmr pftvfrwcpg
       61 armrfkktev cqtcsrlknv cqtclldley glpiqvrdaa lkikddlprs dvnkeyyvqn
      121 idseigkidp tspagavgks aaasdllmkl artspyykrn rphicsfwvk geckrgeecp
      181 yrhekptdpd dpladqnikd ryygvndpva dklmrraaam pkldppedks ittlyignlg
      241 dvltekqlrd hfyqygeirs itmvprqqca fiqytqrsaa eaaaertfnk lilggrrlti
      301 kwgrsqgrqt vsaaeatrei lepvpglpga lppppesmgn nffnlqttpg mmppmmippp
      361 pvapqfmfpp qmaaaaaaaa tpifppgttp ihypsqdpsr mgasqgigkp wpee
//
LOCUS       XP_034191234             402 aa            linear   INV 03-SEP-2025
DEFINITION  putative pyruvate dehydrogenase E1 component subunit alpha,
            mitochondrial isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034191234
VERSION     XP_034191234.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335343.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..402
                     /product="putative pyruvate dehydrogenase E1 component
                     subunit alpha, mitochondrial isoform X1"
                     /calculated_mol_wt=44352
     Region          36..383
                     /region_name="TPP_enzymes"
                     /note="Thiamine pyrophosphate (TPP) enzyme family,
                     TPP-binding module; found in many key metabolic enzymes
                     which use TPP (also known as thiamine diphosphate) as a
                     cofactor. These enzymes include, among others, the E1
                     components of the pyruvate, the acetoin and...; cl01629"
                     /db_xref="CDD:470272"
     Site            order(93,145,170..171,202,204..207,210,214,218,233..236,
                     250,253,301,305)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(123..124,170,172,200..203,230,232,297)
                     /site_type="other"
                     /note="TPP-binding site [chemical binding]"
                     /db_xref="CDD:238958"
     Site            order(165,167,169,174,177..178,181..182,184..185,188,
                     207..208,214..215,218)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(292..298,300..310,319..321)
                     /site_type="phosphorylation"
                     /note="phosphorylation loop region [posttranslational
                     modification]"
                     /db_xref="CDD:238958"
     CDS             1..402
                     /gene="LOC117609253"
                     /coded_by="XM_034335343.2:163..1371"
                     /db_xref="GeneID:117609253"
ORIGIN      
        1 mipncirnig aqtskrnfgs wnevvsyffn kknnyateas fetkpfrlhk ldsgpsthvt
       61 vtrdealdmy rklhtirrme taagnlykek ivrgfchlys gqeacavgik aalrsqdavi
      121 tayrahgwty lmgiepfgvl aeltgrksgn akgkggsmhm ycknfyggng ivgaqvplgv
      181 giafaqkymn tggvcvalyg dgaanqgqvf evynmaklwd vpcifvcenn gygmgtsvdr
      241 asastdyhtr gdyipgiwvd gmdvlavrea trfavdfcts gkgpiiletv tyrysghsms
      301 dpgtsyrtre evqevrqtrd pitgfkeril nsnlitqeei ktmeneirkm iddavkaaka
      361 dseiplnelt adiyancvek eirnitpfnp lshtrlgpav na
//
LOCUS       XP_034191235             396 aa            linear   INV 03-SEP-2025
DEFINITION  putative pyruvate dehydrogenase E1 component subunit alpha,
            mitochondrial isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034191235
VERSION     XP_034191235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335344.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..396
                     /product="putative pyruvate dehydrogenase E1 component
                     subunit alpha, mitochondrial isoform X2"
                     /calculated_mol_wt=43631
     Region          30..377
                     /region_name="TPP_enzymes"
                     /note="Thiamine pyrophosphate (TPP) enzyme family,
                     TPP-binding module; found in many key metabolic enzymes
                     which use TPP (also known as thiamine diphosphate) as a
                     cofactor. These enzymes include, among others, the E1
                     components of the pyruvate, the acetoin and...; cl01629"
                     /db_xref="CDD:470272"
     Site            order(87,139,164..165,196,198..201,204,208,212,227..230,
                     244,247,295,299)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(117..118,164,166,194..197,224,226,291)
                     /site_type="other"
                     /note="TPP-binding site [chemical binding]"
                     /db_xref="CDD:238958"
     Site            order(159,161,163,168,171..172,175..176,178..179,182,
                     201..202,208..209,212)
                     /site_type="other"
                     /note="heterodimer interface [polypeptide binding]"
                     /db_xref="CDD:238958"
     Site            order(286..292,294..304,313..315)
                     /site_type="phosphorylation"
                     /note="phosphorylation loop region [posttranslational
                     modification]"
                     /db_xref="CDD:238958"
     CDS             1..396
                     /gene="LOC117609253"
                     /coded_by="XM_034335344.2:160..1350"
                     /db_xref="GeneID:117609253"
ORIGIN      
        1 mipncirnig aqtskrnvvs yffnkknnya teasfetkpf rlhkldsgps thvtvtrdea
       61 ldmyrklhti rrmetaagnl ykekivrgfc hlysgqeaca vgikaalrsq davitayrah
      121 gwtylmgiep fgvlaeltgr ksgnakgkgg smhmycknfy ggngivgaqv plgvgiafaq
      181 kymntggvcv alygdgaanq gqvfevynma klwdvpcifv cenngygmgt svdrasastd
      241 yhtrgdyipg iwvdgmdvla vreatrfavd fctsgkgpii letvtyrysg hsmsdpgtsy
      301 rtreevqevr qtrdpitgfk erilnsnlit qeeiktmene irkmiddavk aakadseipl
      361 neltadiyan cvekeirnit pfnplshtrl gpavna
//
LOCUS       XP_034191236             153 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609254 [Osmia lignaria lignaria].
ACCESSION   XP_034191236
VERSION     XP_034191236.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335345.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..153
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..153
                     /product="uncharacterized protein LOC117609254"
                     /calculated_mol_wt=16124
     CDS             1..153
                     /gene="LOC117609254"
                     /coded_by="XM_034335345.2:164..625"
                     /db_xref="GeneID:117609254"
ORIGIN      
        1 maaagstnsg tifqklglkp itkcslvkfy apafgvasyt alsvnvmnps lvirvfpkkd
       61 itnfllgsal lgtgsyiytr dhmkaatida kiiysttgav llsfgsvliw avlrsvvppn
      121 ptlctiigia sgitfikigs tyldfvdgqi qkk
//
LOCUS       XP_076548227             123 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117609110 [Osmia lignaria lignaria].
ACCESSION   XP_076548227
VERSION     XP_076548227.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692112.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="uncharacterized protein LOC117609110"
                     /calculated_mol_wt=14553
     CDS             1..123
                     /gene="LOC117609110"
                     /coded_by="XM_076692112.1:349..720"
                     /db_xref="GeneID:117609110"
ORIGIN      
        1 mninymysrq ylneqykhsi ilsslfvgmc rcyeklashr qllnyhrdym kststnilvs
       61 dkfnfggeky mgvnylypyi steysdyfly eirseelflv htstpvarsf lnssnkiate
      121 ifh
//
LOCUS       XP_034191238              64 aa            linear   INV 03-SEP-2025
DEFINITION  stress-associated endoplasmic reticulum protein 2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034191238
VERSION     XP_034191238.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034335347.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..64
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..64
                     /product="stress-associated endoplasmic reticulum protein
                     2"
                     /calculated_mol_wt=6855
     Region          2..58
                     /region_name="RAMP4"
                     /note="Ribosome associated membrane protein RAMP4;
                     pfam06624"
                     /db_xref="CDD:461965"
     CDS             1..64
                     /gene="LOC117609255"
                     /coded_by="XM_034335347.2:59..253"
                     /db_xref="GeneID:117609255"
ORIGIN      
        1 mapkqrmria neratknitl rgnvpksskp qddgspigpc llalflfvvc gsavfqiiqs
       61 irma
//
LOCUS       XP_034183110             328 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605659 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183110
VERSION     XP_034183110.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327219.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..328
                     /product="uncharacterized protein LOC117605659 isoform X1"
                     /calculated_mol_wt=37147
     Region          <241..>318
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          248..270
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(255,257,259,261..262,265..266,269,285,287,291..292,
                     295..296,299,313,315,317,319..320,323..324,327)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          278..300
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          308..328
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..328
                     /gene="LOC117605659"
                     /coded_by="XM_034327219.2:2762..3748"
                     /db_xref="GeneID:117605659"
ORIGIN      
        1 mvfmdesels skrwasaprr pdspcflgap tsreasgppv qlepvdlsvk tpvvlqvpry
       61 spaaiitaas rkipspsttp tpppfsisig eflthpttne lviakfvspp lpplgetawe
      121 rnrererere rvrerstene esgirerepw drekerdrdr drqwdrerdr erdtsarmme
      181 pldpvfvsss aallalqrik etslvfhkrp tgigiggrgn agvggnggsl pvdsecgdal
      241 krrkvhrcdv agcdkvytks shlkahkrth tgekpyqctw egctwkfars deltrhyrkh
      301 tgqkpfkchl cqrsfsrsdh lslhmkrh
//
LOCUS       XP_034183111             311 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605659 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183111
VERSION     XP_034183111.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327220.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..311
                     /product="uncharacterized protein LOC117605659 isoform X2"
                     /calculated_mol_wt=35248
     Region          <224..>301
                     /region_name="COG5048"
                     /note="FOG: Zn-finger [General function prediction only]"
                     /db_xref="CDD:227381"
     Region          231..253
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(238,240,242,244..245,248..249,252,268,270,274..275,
                     278..279,282,296,298,300,302..303,306..307,310)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          261..283
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Region          291..311
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..311
                     /gene="LOC117605659"
                     /coded_by="XM_034327220.2:2746..3681"
                     /db_xref="GeneID:117605659"
ORIGIN      
        1 mvfmdesels skrwasaprr pdspcflgap tsreasgppv qlepvdlsvk tpvvlqvpry
       61 spaaiitaas rkipspsttp tpppfsisiv spplpplget awernrerer erervrerst
      121 eneesgirer epwdrekerd rdrdrqwdre rdrerdtsar mmepldpvfv sssaallalq
      181 riketslvfh krptgigigg rgnagvggng gslpvdsecg dalkrrkvhr cdvagcdkvy
      241 tksshlkahk rthtgekpyq ctwegctwkf arsdeltrhy rkhtgqkpfk chlcqrsfsr
      301 sdhlslhmkr h
//
LOCUS       XP_076548228              95 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306065 [Osmia lignaria lignaria].
ACCESSION   XP_076548228
VERSION     XP_076548228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692113.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..95
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..95
                     /product="uncharacterized protein LOC143306065"
                     /calculated_mol_wt=10590
     Region          37..74
                     /region_name="Pacifastin_I"
                     /note="Pacifastin inhibitor (LCMII); pfam05375"
                     /db_xref="CDD:253170"
     CDS             1..95
                     /gene="LOC143306065"
                     /coded_by="XM_076692113.1:60..347"
                     /db_xref="GeneID:143306065"
ORIGIN      
        1 mfikfftfll llaivssmsi dvgtersnea dlsharqcip geryfdgcnw cschqggfia
       61 ctlmacetyn aktdrfeatk elpspdnfgk kyesd
//
LOCUS       XP_034183001            1161 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605593 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183001
VERSION     XP_034183001.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327110.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183001.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1161
                     /product="uncharacterized protein LOC117605593 isoform X1"
                     /calculated_mol_wt=128200
     Region          225..310
                     /region_name="RA_MRL_MIG10"
                     /note="Ras-associating (RA) domain found in Caenorhabditis
                     elegans abnormal cell migration protein 10 (MIG-10) and
                     similar proteins; cd16138"
                     /db_xref="CDD:340555"
     Site            order(230,235..242,258..261,276,294..295,298)
                     /site_type="other"
                     /note="RA-PH-RAP1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340555"
     Region          350..474
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          843..>1010
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..1161
                     /gene="LOC117605593"
                     /coded_by="XM_034327110.2:1053..4538"
                     /db_xref="GeneID:117605593"
ORIGIN      
        1 mlcgtfrkkr rhpgdeyrlv rsntmpkils akegslvsvr rtkssrvars pqlrdllqmg
       61 ldnvssatlr pfnsdintpr idsyrfsman ledsqdvdld ailgelcale rrcdgdiaat
      121 papdsqrqgr pnstritaad ntdigknega mrtdspdnds afsdtvsmls sessasssgs
      181 ghkppqtamh tapqqqshql mdaagrvkae kirlalekir easvqklfik aftldgsgks
      241 llvdegmsva hvcrlladkn hvpmdpkwav vehlpdlfme rvyedhellv enlllwtrds
      301 knkllfverp ektqlfltpe rfllgpsdrs sgeyddhsrn illeeffsss nvgvpevegp
      361 lylksdskkg wkryhfilra sglyywpkek artardlvcl atfdvnqiyy gigwkkkyka
      421 ptdfcfavkh prlqqpkstk yikflcaedn aslerwmvgi rvakygrqlm enyrtlvdel
      481 aqedldilah arscsvssia vpaqnqtqyn tindnarqft enfrhnveva itrqydgqrq
      541 iynseqrqsy nndgrlsras sssssgclsd gapsscevaf ecgefptgti krkpsmnpkl
      601 pltsitrqlk evgetvrdep dscpsptssg sgtltrrhsr rrsgtdsdgs gtlkrhhrsg
      661 natpvspvpp gtpvrerasp mgysrsesqe sktptspipt cmmdsitslp pppspsrvae
      721 etesdneplp ppppemfrsn lsldslpppp apgelpmcnt peltgsslsl aslppppspl
      781 vgetgtirra rpkqstptns itpegtptha psrstnnqqi ysttnnlqnn snsqnyntns
      841 qnvlhinnta nsmasphssy pgsnastpty apsspnftsp ppfvpppayg tqqhnsssls
      901 rqnskvesly gthpgvhqhn stvqpirpnp nmdtvrrsam kqgsghyaap pylaelkaas
      961 spqpqrrvti qepptspksk tgtgkkitfn lppqqepgsp alpqrkpmpp rrsdstrlts
     1021 pkklaasdqa ppgdflkdlq rvmrkkwqva qkckldsttt phevlgfrdp ppavadyret
     1081 nvsnwvqehy gadnlyenvy atdphapvey assparqatv rfadenrsin ivnaiaskrr
     1141 ppppppkrae tthltttram h
//
LOCUS       XP_034183003            1142 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605593 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183003
VERSION     XP_034183003.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327112.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183003.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1142
                     /product="uncharacterized protein LOC117605593 isoform X2"
                     /calculated_mol_wt=126077
     Region          206..291
                     /region_name="RA_MRL_MIG10"
                     /note="Ras-associating (RA) domain found in Caenorhabditis
                     elegans abnormal cell migration protein 10 (MIG-10) and
                     similar proteins; cd16138"
                     /db_xref="CDD:340555"
     Site            order(211,216..223,239..242,257,275..276,279)
                     /site_type="other"
                     /note="RA-PH-RAP1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340555"
     Region          331..455
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          824..>991
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..1142
                     /gene="LOC117605593"
                     /coded_by="XM_034327112.2:1619..5047"
                     /db_xref="GeneID:117605593"
ORIGIN      
        1 mdwirrqdik eespetptal tpdepdecyf eedtsidegm gldnvssatl rpfnsdintp
       61 ridsyrfsma nledsqdvdl dailgelcal errcdgdiaa tpapdsqrqg rpnstritaa
      121 dntdigkneg amrtdspdnd safsdtvsml ssessasssg sghkppqtam htapqqqshq
      181 lmdaagrvka ekirlaleki reasvqklfi kaftldgsgk sllvdegmsv ahvcrlladk
      241 nhvpmdpkwa vvehlpdlfm ervyedhell venlllwtrd sknkllfver pektqlfltp
      301 erfllgpsdr ssgeyddhsr nilleeffss snvgvpeveg plylksdskk gwkryhfilr
      361 asglyywpke kartardlvc latfdvnqiy ygigwkkkyk aptdfcfavk hprlqqpkst
      421 kyikflcaed naslerwmvg irvakygrql menyrtlvde laqedldila harscsvssi
      481 avpaqnqtqy ntindnarqf tenfrhnvev aitrqydgqr qiynseqrqs ynndgrlsra
      541 ssssssgcls dgapssceva fecgefptgt ikrkpsmnpk lpltsitrql kevgetvrde
      601 pdscpsptss gsgtltrrhs rrrsgtdsdg sgtlkrhhrs gnatpvspvp pgtpvreras
      661 pmgysrsesq esktptspip tcmmdsitsl ppppspsrva eetesdnepl pppppemfrs
      721 nlsldslppp papgelpmcn tpeltgssls laslppppsp lvgetgtirr arpkqstptn
      781 sitpegtpth apsrstnnqq iysttnnlqn nsnsqnyntn sqnvlhinnt ansmasphss
      841 ypgsnastpt yapsspnfts pppfvpppay gtqqhnsssl srqnskvesl ygthpgvhqh
      901 nstvqpirpn pnmdtvrrsa mkqgsghyaa ppylaelkaa sspqpqrrvt iqepptspks
      961 ktgtgkkitf nlppqqepgs palpqrkpmp prrsdstrlt spkklaasdq appgdflkdl
     1021 qrvmrkkwqv aqkckldstt tphevlgfrd pppavadyre tnvsnwvqeh ygadnlyenv
     1081 yatdphapve yassparqat vrfadenrsi nivnaiaskr rppppppkra etthltttra
     1141 mh
//
LOCUS       XP_076548229            1135 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605593 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548229
VERSION     XP_076548229.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692114.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1135
                     /product="uncharacterized protein LOC117605593 isoform X3"
                     /calculated_mol_wt=125243
     Region          199..284
                     /region_name="RA_MRL_MIG10"
                     /note="Ras-associating (RA) domain found in Caenorhabditis
                     elegans abnormal cell migration protein 10 (MIG-10) and
                     similar proteins; cd16138"
                     /db_xref="CDD:340555"
     Site            order(204,209..216,232..235,250,268..269,272)
                     /site_type="other"
                     /note="RA-PH-RAP1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340555"
     Region          324..448
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          817..>984
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..1135
                     /gene="LOC117605593"
                     /coded_by="XM_076692114.1:334..3741"
                     /db_xref="GeneID:117605593"
ORIGIN      
        1 meryeeeged sdnetdpeql lnewlgelds ltvgldnvss atlrpfnsdi ntpridsyrf
       61 smanledsqd vdldailgel calerrcdgd iaatpapdsq rqgrpnstri taadntdigk
      121 negamrtdsp dndsafsdtv smlssessas ssgsghkppq tamhtapqqq shqlmdaagr
      181 vkaekirlal ekireasvqk lfikaftldg sgksllvdeg msvahvcrll adknhvpmdp
      241 kwavvehlpd lfmervyedh ellvenlllw trdsknkllf verpektqlf ltperfllgp
      301 sdrssgeydd hsrnilleef fsssnvgvpe vegplylksd skkgwkryhf ilrasglyyw
      361 pkekartard lvclatfdvn qiyygigwkk kykaptdfcf avkhprlqqp kstkyikflc
      421 aednaslerw mvgirvakyg rqlmenyrtl vdelaqedld ilaharscsv ssiavpaqnq
      481 tqyntindna rqftenfrhn vevaitrqyd gqrqiynseq rqsynndgrl srassssssg
      541 clsdgapssc evafecgefp tgtikrkpsm npklpltsit rqlkevgetv rdepdscpsp
      601 tssgsgtltr rhsrrrsgtd sdgsgtlkrh hrsgnatpvs pvppgtpvre raspmgysrs
      661 esqesktpts piptcmmdsi tslppppsps rvaeetesdn eplpppppem frsnlsldsl
      721 ppppapgelp mcntpeltgs slslaslppp psplvgetgt irrarpkqst ptnsitpegt
      781 pthapsrstn nqqiysttnn lqnnsnsqny ntnsqnvlhi nntansmasp hssypgsnas
      841 tptyapsspn ftspppfvpp paygtqqhns sslsrqnskv eslygthpgv hqhnstvqpi
      901 rpnpnmdtvr rsamkqgsgh yaappylael kaasspqpqr rvtiqeppts pksktgtgkk
      961 itfnlppqqe pgspalpqrk pmpprrsdst rltspkklaa sdqappgdfl kdlqrvmrkk
     1021 wqvaqkckld stttphevlg frdpppavad yretnvsnwv qehygadnly envyatdpha
     1081 pveyasspar qatvrfaden rsinivnaia skrrpppppp kraetthltt tramh
//
LOCUS       XP_034183005            1117 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605593 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183005
VERSION     XP_034183005.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327114.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183005.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1117
                     /product="uncharacterized protein LOC117605593 isoform X4"
                     /calculated_mol_wt=123338
     Region          181..266
                     /region_name="RA_MRL_MIG10"
                     /note="Ras-associating (RA) domain found in Caenorhabditis
                     elegans abnormal cell migration protein 10 (MIG-10) and
                     similar proteins; cd16138"
                     /db_xref="CDD:340555"
     Site            order(186,191..198,214..217,232,250..251,254)
                     /site_type="other"
                     /note="RA-PH-RAP1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340555"
     Region          306..430
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          799..>966
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..1117
                     /gene="LOC117605593"
                     /coded_by="XM_034327114.2:205..3558"
                     /db_xref="GeneID:117605593"
ORIGIN      
        1 mdrffrwksh kaqedgldnv ssatlrpfns dintpridsy rfsmanleds qdvdldailg
       61 elcalerrcd gdiaatpapd sqrqgrpnst ritaadntdi gknegamrtd spdndsafsd
      121 tvsmlssess asssgsghkp pqtamhtapq qqshqlmdaa grvkaekirl alekireasv
      181 qklfikaftl dgsgksllvd egmsvahvcr lladknhvpm dpkwavvehl pdlfmervye
      241 dhellvenll lwtrdsknkl lfverpektq lfltperfll gpsdrssgey ddhsrnille
      301 effsssnvgv pevegplylk sdskkgwkry hfilrasgly ywpkekarta rdlvclatfd
      361 vnqiyygigw kkkykaptdf cfavkhprlq qpkstkyikf lcaednasle rwmvgirvak
      421 ygrqlmenyr tlvdelaqed ldilaharsc svssiavpaq nqtqyntind narqftenfr
      481 hnvevaitrq ydgqrqiyns eqrqsynndg rlsrasssss sgclsdgaps scevafecge
      541 fptgtikrkp smnpklplts itrqlkevge tvrdepdscp sptssgsgtl trrhsrrrsg
      601 tdsdgsgtlk rhhrsgnatp vspvppgtpv reraspmgys rsesqesktp tspiptcmmd
      661 sitslpppps psrvaeetes dneplppppp emfrsnlsld slppppapge lpmcntpelt
      721 gsslslaslp pppsplvget gtirrarpkq stptnsitpe gtpthapsrs tnnqqiystt
      781 nnlqnnsnsq nyntnsqnvl hinntansma sphssypgsn astptyapss pnftspppfv
      841 pppaygtqqh nssslsrqns kveslygthp gvhqhnstvq pirpnpnmdt vrrsamkqgs
      901 ghyaappyla elkaasspqp qrrvtiqepp tspksktgtg kkitfnlppq qepgspalpq
      961 rkpmpprrsd strltspkkl aasdqappgd flkdlqrvmr kkwqvaqkck ldstttphev
     1021 lgfrdpppav adyretnvsn wvqehygadn lyenvyatdp hapveyassp arqatvrfad
     1081 enrsinivna iaskrrpppp ppkraetthl tttramh
//
LOCUS       XP_034183006            1087 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605593 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183006
VERSION     XP_034183006.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327115.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183006.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1087
                     /product="uncharacterized protein LOC117605593 isoform X5"
                     /calculated_mol_wt=119661
     Region          151..236
                     /region_name="RA_MRL_MIG10"
                     /note="Ras-associating (RA) domain found in Caenorhabditis
                     elegans abnormal cell migration protein 10 (MIG-10) and
                     similar proteins; cd16138"
                     /db_xref="CDD:340555"
     Site            order(156,161..168,184..187,202,220..221,224)
                     /site_type="other"
                     /note="RA-PH-RAP1 interaction site [polypeptide binding]"
                     /db_xref="CDD:340555"
     Region          276..400
                     /region_name="PH_APBB1IP"
                     /note="Amyloid beta (A4) Precursor protein-Binding, family
                     B, member 1 Interacting Protein pleckstrin homology (PH)
                     domain; cd01259"
                     /db_xref="CDD:269961"
     Region          769..>936
                     /region_name="Atrophin-1"
                     /note="Atrophin-1 family; pfam03154"
                     /db_xref="CDD:460830"
     CDS             1..1087
                     /gene="LOC117605593"
                     /coded_by="XM_034327115.2:766..4029"
                     /db_xref="GeneID:117605593"
ORIGIN      
        1 mltnfgsnes ilgylsnkds qdvdldailg elcalerrcd gdiaatpapd sqrqgrpnst
       61 ritaadntdi gknegamrtd spdndsafsd tvsmlssess asssgsghkp pqtamhtapq
      121 qqshqlmdaa grvkaekirl alekireasv qklfikaftl dgsgksllvd egmsvahvcr
      181 lladknhvpm dpkwavvehl pdlfmervye dhellvenll lwtrdsknkl lfverpektq
      241 lfltperfll gpsdrssgey ddhsrnille effsssnvgv pevegplylk sdskkgwkry
      301 hfilrasgly ywpkekarta rdlvclatfd vnqiyygigw kkkykaptdf cfavkhprlq
      361 qpkstkyikf lcaednasle rwmvgirvak ygrqlmenyr tlvdelaqed ldilaharsc
      421 svssiavpaq nqtqyntind narqftenfr hnvevaitrq ydgqrqiyns eqrqsynndg
      481 rlsrasssss sgclsdgaps scevafecge fptgtikrkp smnpklplts itrqlkevge
      541 tvrdepdscp sptssgsgtl trrhsrrrsg tdsdgsgtlk rhhrsgnatp vspvppgtpv
      601 reraspmgys rsesqesktp tspiptcmmd sitslpppps psrvaeetes dneplppppp
      661 emfrsnlsld slppppapge lpmcntpelt gsslslaslp pppsplvget gtirrarpkq
      721 stptnsitpe gtpthapsrs tnnqqiystt nnlqnnsnsq nyntnsqnvl hinntansma
      781 sphssypgsn astptyapss pnftspppfv pppaygtqqh nssslsrqns kveslygthp
      841 gvhqhnstvq pirpnpnmdt vrrsamkqgs ghyaappyla elkaasspqp qrrvtiqepp
      901 tspksktgtg kkitfnlppq qepgspalpq rkpmpprrsd strltspkkl aasdqappgd
      961 flkdlqrvmr kkwqvaqkck ldstttphev lgfrdpppav adyretnvsn wvqehygadn
     1021 lyenvyatdp hapveyassp arqatvrfad enrsinivna iaskrrpppp ppkraetthl
     1081 tttramh
//
LOCUS       XP_034183008            1014 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase mind bomb 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183008
VERSION     XP_034183008.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327117.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1014
                     /product="E3 ubiquitin-protein ligase mind bomb 1 isoform
                     X1"
                     /calculated_mol_wt=110657
     Region          20..77
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          88..132
                     /region_name="ZZ_Mind_bomb"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     Drosophila Mind bomb (D-mib) and related proteins. The ZZ
                     motif coordinates two zinc ions and most likely
                     participates in ligand binding or molecular scaffolding.
                     Mind bomb is an E3 ubiqitin ligase that has...; cd02339"
                     /db_xref="CDD:239079"
     Site            order(90,93,105,108,114,117,123,127)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(90,93,114,117)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(91,102,104,110,112)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(103,118,129,132)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(105,108,123,127)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239079"
     Region          159..224
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          252..317
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          336..400
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          460..724
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..531
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(469,473..474,477..479,481..482,486,489,531,533,535,
                     539..540,543..545,547..548,552,555,564,566,568,572..573,
                     576..578,580..581,585,588,597)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          533..564
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          566..597
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          599..633
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(635,637,641..642,645..647,649..650,654,657,667,669,
                     671,675..676,679..681,683..684,688,691,700,702,704,
                     708..709,712..713,715,727,731,734)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          635..667
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          669..700
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          702..737
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          804..841
                     /region_name="RING-HC_MIB1_rpt1"
                     /note="first RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16724"
                     /db_xref="CDD:438384"
     Region          851..888
                     /region_name="RING-HC_MIB1_rpt2"
                     /note="second RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16725"
                     /db_xref="CDD:438385"
     Region          969..1014
                     /region_name="RING-HC_MIB1_rpt3"
                     /note="third RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16727"
                     /db_xref="CDD:438387"
     CDS             1..1014
                     /gene="mib1"
                     /coded_by="XM_034327117.2:116..3160"
                     /db_xref="GeneID:117605594"
ORIGIN      
        1 measastgtr tmrfmmegvg arvirgpewk wgkqdggegh vgtvrnfesp eevvvvwdng
       61 taanyrcsga fdlrildsap tgvkhdgtmc dtcrqqpifg irwkcaecgn ydlcsicyhg
      121 dkhhlrhrfy riatpgserv lleprrkskk iairgifpga rvvrgvdwqw edqdggngrr
      181 gkvneiqdws aasprsaayv iwdngaknly rvgfegmadl kvvndakgqt vyrdhlpllg
      241 eqgpgrtgph glqigdqvnv dleleivqsl qhghggwtdg mfeclgttgt vvgidedhdi
      301 vvsypsgnrw tfnpavltkv qipvpvtsss sdnqtfavgd lvqicndlek ikllqrghge
      361 waeamaptmg kigrvlqiyh dgdlkvevcs tswtynpqav tkvassdgsv pgnssgerls
      421 allkklfeth vsgdvneelv ktaangdaak ceeclkrpea dvngvfaght alqaasqngh
      481 levikillry kadveiedkd gdravhhaaf gdepgvmall agagadlnar nkrrqtalhi
      541 avnkghagvv rtllelgchp slqdsegdtp lhdaiskkrd dmlallldha aditltnnng
      601 fnalhhaalr gnpsamrvll sklprpwivd ekkddgytal hlaalnnhve vaeqlaragk
      661 adldlqnvnl qtalhlaver qhtqivrllv reganlnvad kdgdtplhea lryhtlsqlr
      721 qlqdvqdvgr llmglgaqgq dkksssfiac flaahgadle lknkkgqtpl dlcpdpnlck
      781 tlttchkdre shdietaaps gtideclvcs dgkremlftp cghvaccnac aprvkkclic
      841 rdnvltrtki eecvvcsdrk asmlfrpcgh mcacescatl mkkcvqcrsq iqhmlplsmc
      901 cggggnvtyv kginasgsis dvkndaeeet ipqntgsgea ilmnngsrdt shsdiqklqq
      961 qlqdikeqtm cpvcldrlkn miflcghgtc qmcgdrmsec picrkavdkr illy
//
LOCUS       XP_034183007            1014 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase mind bomb 1 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183007
VERSION     XP_034183007.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327116.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1014
                     /product="E3 ubiquitin-protein ligase mind bomb 1 isoform
                     X1"
                     /calculated_mol_wt=110657
     Region          20..77
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          88..132
                     /region_name="ZZ_Mind_bomb"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     Drosophila Mind bomb (D-mib) and related proteins. The ZZ
                     motif coordinates two zinc ions and most likely
                     participates in ligand binding or molecular scaffolding.
                     Mind bomb is an E3 ubiqitin ligase that has...; cd02339"
                     /db_xref="CDD:239079"
     Site            order(90,93,105,108,114,117,123,127)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(90,93,114,117)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(91,102,104,110,112)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(103,118,129,132)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(105,108,123,127)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239079"
     Region          159..224
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          252..317
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          336..400
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          460..724
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..531
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(469,473..474,477..479,481..482,486,489,531,533,535,
                     539..540,543..545,547..548,552,555,564,566,568,572..573,
                     576..578,580..581,585,588,597)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          533..564
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          566..597
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          599..633
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(635,637,641..642,645..647,649..650,654,657,667,669,
                     671,675..676,679..681,683..684,688,691,700,702,704,
                     708..709,712..713,715,727,731,734)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          635..667
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          669..700
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          702..737
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          804..841
                     /region_name="RING-HC_MIB1_rpt1"
                     /note="first RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16724"
                     /db_xref="CDD:438384"
     Region          851..888
                     /region_name="RING-HC_MIB1_rpt2"
                     /note="second RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16725"
                     /db_xref="CDD:438385"
     Region          969..1014
                     /region_name="RING-HC_MIB1_rpt3"
                     /note="third RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16727"
                     /db_xref="CDD:438387"
     CDS             1..1014
                     /gene="mib1"
                     /coded_by="XM_034327116.2:262..3306"
                     /db_xref="GeneID:117605594"
ORIGIN      
        1 measastgtr tmrfmmegvg arvirgpewk wgkqdggegh vgtvrnfesp eevvvvwdng
       61 taanyrcsga fdlrildsap tgvkhdgtmc dtcrqqpifg irwkcaecgn ydlcsicyhg
      121 dkhhlrhrfy riatpgserv lleprrkskk iairgifpga rvvrgvdwqw edqdggngrr
      181 gkvneiqdws aasprsaayv iwdngaknly rvgfegmadl kvvndakgqt vyrdhlpllg
      241 eqgpgrtgph glqigdqvnv dleleivqsl qhghggwtdg mfeclgttgt vvgidedhdi
      301 vvsypsgnrw tfnpavltkv qipvpvtsss sdnqtfavgd lvqicndlek ikllqrghge
      361 waeamaptmg kigrvlqiyh dgdlkvevcs tswtynpqav tkvassdgsv pgnssgerls
      421 allkklfeth vsgdvneelv ktaangdaak ceeclkrpea dvngvfaght alqaasqngh
      481 levikillry kadveiedkd gdravhhaaf gdepgvmall agagadlnar nkrrqtalhi
      541 avnkghagvv rtllelgchp slqdsegdtp lhdaiskkrd dmlallldha aditltnnng
      601 fnalhhaalr gnpsamrvll sklprpwivd ekkddgytal hlaalnnhve vaeqlaragk
      661 adldlqnvnl qtalhlaver qhtqivrllv reganlnvad kdgdtplhea lryhtlsqlr
      721 qlqdvqdvgr llmglgaqgq dkksssfiac flaahgadle lknkkgqtpl dlcpdpnlck
      781 tlttchkdre shdietaaps gtideclvcs dgkremlftp cghvaccnac aprvkkclic
      841 rdnvltrtki eecvvcsdrk asmlfrpcgh mcacescatl mkkcvqcrsq iqhmlplsmc
      901 cggggnvtyv kginasgsis dvkndaeeet ipqntgsgea ilmnngsrdt shsdiqklqq
      961 qlqdikeqtm cpvcldrlkn miflcghgtc qmcgdrmsec picrkavdkr illy
//
LOCUS       XP_034183009             984 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase mind bomb 1 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183009
VERSION     XP_034183009.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327118.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..984
                     /product="E3 ubiquitin-protein ligase mind bomb 1 isoform
                     X2"
                     /calculated_mol_wt=107596
     Region          20..77
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          88..132
                     /region_name="ZZ_Mind_bomb"
                     /note="Zinc finger, ZZ type. Zinc finger present in
                     Drosophila Mind bomb (D-mib) and related proteins. The ZZ
                     motif coordinates two zinc ions and most likely
                     participates in ligand binding or molecular scaffolding.
                     Mind bomb is an E3 ubiqitin ligase that has...; cd02339"
                     /db_xref="CDD:239079"
     Site            order(90,93,105,108,114,117,123,127)
                     /site_type="other"
                     /note="Zinc-binding sites [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(90,93,114,117)
                     /site_type="other"
                     /note="zinc cluster 1 [ion binding]"
                     /db_xref="CDD:239079"
     Site            order(91,102,104,110,112)
                     /site_type="active"
                     /note="putative charged binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(103,118,129,132)
                     /site_type="active"
                     /note="putative hydrophobic binding surface [active]"
                     /db_xref="CDD:239079"
     Site            order(105,108,123,127)
                     /site_type="other"
                     /note="zinc cluster 2 [ion binding]"
                     /db_xref="CDD:239079"
     Region          159..224
                     /region_name="MIB_HERC2"
                     /note="Mib_herc2; pfam06701"
                     /db_xref="CDD:461991"
     Region          252..317
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          336..400
                     /region_name="SH3_15"
                     /note="Mind bomb SH3 repeat domain; pfam18346"
                     /db_xref="CDD:465720"
     Region          460..724
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          468..531
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(469,473..474,477..479,481..482,486,489,531,533,535,
                     539..540,543..545,547..548,552,555,564,566,568,572..573,
                     576..578,580..581,585,588,597)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          533..564
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          566..597
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          599..633
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(635,637,641..642,645..647,649..650,654,657,667,669,
                     671,675..676,679..681,683..684,688,691,700,702,704,
                     708..709,712..713,715,727,731,734)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          635..667
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          669..700
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          702..737
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          804..841
                     /region_name="RING-HC_MIB1_rpt1"
                     /note="first RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16724"
                     /db_xref="CDD:438384"
     Region          851..887
                     /region_name="RING-HC_MIB1_rpt2"
                     /note="second RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16725"
                     /db_xref="CDD:438385"
     Region          939..984
                     /region_name="RING-HC_MIB1_rpt3"
                     /note="third RING finger, HC subclass, found in mind bomb
                     1 (MIB1) and similar proteins; cd16727"
                     /db_xref="CDD:438387"
     CDS             1..984
                     /gene="mib1"
                     /coded_by="XM_034327118.2:116..3070"
                     /db_xref="GeneID:117605594"
ORIGIN      
        1 measastgtr tmrfmmegvg arvirgpewk wgkqdggegh vgtvrnfesp eevvvvwdng
       61 taanyrcsga fdlrildsap tgvkhdgtmc dtcrqqpifg irwkcaecgn ydlcsicyhg
      121 dkhhlrhrfy riatpgserv lleprrkskk iairgifpga rvvrgvdwqw edqdggngrr
      181 gkvneiqdws aasprsaayv iwdngaknly rvgfegmadl kvvndakgqt vyrdhlpllg
      241 eqgpgrtgph glqigdqvnv dleleivqsl qhghggwtdg mfeclgttgt vvgidedhdi
      301 vvsypsgnrw tfnpavltkv qipvpvtsss sdnqtfavgd lvqicndlek ikllqrghge
      361 waeamaptmg kigrvlqiyh dgdlkvevcs tswtynpqav tkvassdgsv pgnssgerls
      421 allkklfeth vsgdvneelv ktaangdaak ceeclkrpea dvngvfaght alqaasqngh
      481 levikillry kadveiedkd gdravhhaaf gdepgvmall agagadlnar nkrrqtalhi
      541 avnkghagvv rtllelgchp slqdsegdtp lhdaiskkrd dmlallldha aditltnnng
      601 fnalhhaalr gnpsamrvll sklprpwivd ekkddgytal hlaalnnhve vaeqlaragk
      661 adldlqnvnl qtalhlaver qhtqivrllv reganlnvad kdgdtplhea lryhtlsqlr
      721 qlqdvqdvgr llmglgaqgq dkksssfiac flaahgadle lknkkgqtpl dlcpdpnlck
      781 tlttchkdre shdietaaps gtideclvcs dgkremlftp cghvaccnac aprvkkclic
      841 rdnvltrtki eecvvcsdrk asmlfrpcgh mcacescatl mkkcvqcsis dvkndaeeet
      901 ipqntgsgea ilmnngsrdt shsdiqklqq qlqdikeqtm cpvcldrlkn miflcghgtc
      961 qmcgdrmsec picrkavdkr illy
//
LOCUS       XP_034183010             443 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light intermediate chain isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183010
VERSION     XP_034183010.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327119.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..443
                     /product="dynein light intermediate chain isoform X1"
                     /calculated_mol_wt=49544
     Region          23..443
                     /region_name="DLIC"
                     /note="Dynein light intermediate chain (DLIC); pfam05783"
                     /db_xref="CDD:368612"
     CDS             1..443
                     /gene="Dlic"
                     /coded_by="XM_034327119.2:446..1777"
                     /db_xref="GeneID:117605595"
ORIGIN      
        1 matmaidnml qsngfqskkk edaendnlws silaevqnsg nnklpsnkni lvlgdnesgk
       61 ttliaklqgv edpkkgsgle fayidvrddy rddqtrlsvw vldgdpghan llrfalnaer
      121 fphtlvmlva amttpwaild qlqswaallg dhidklpldn dtrqrcrqln ikkwqdytep
      181 gdeldpgspl rrtsrnldde tidnlplpeg vlttnlgldi vvvitktdym stlekehdyk
      241 dehfdfmqqw irrfclqyga glfytsaked kncdllykyl thriyslpfr tpalvvekda
      301 vlipagwdnm kkisilhenl qsmkpddyyr dviaqppinr kcvakevevq aeeeqvflak
      361 qqaallglkd ptrspttrnq astgpviqva spnkkldlkg tgttqsgegv lanffnsllh
      421 kktgtpgspg sfdtgptnkg dhv
//
LOCUS       XP_034183011             443 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light intermediate chain isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183011
VERSION     XP_034183011.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327120.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..443
                     /product="dynein light intermediate chain isoform X1"
                     /calculated_mol_wt=49544
     Region          23..443
                     /region_name="DLIC"
                     /note="Dynein light intermediate chain (DLIC); pfam05783"
                     /db_xref="CDD:368612"
     CDS             1..443
                     /gene="Dlic"
                     /coded_by="XM_034327120.2:400..1731"
                     /db_xref="GeneID:117605595"
ORIGIN      
        1 matmaidnml qsngfqskkk edaendnlws silaevqnsg nnklpsnkni lvlgdnesgk
       61 ttliaklqgv edpkkgsgle fayidvrddy rddqtrlsvw vldgdpghan llrfalnaer
      121 fphtlvmlva amttpwaild qlqswaallg dhidklpldn dtrqrcrqln ikkwqdytep
      181 gdeldpgspl rrtsrnldde tidnlplpeg vlttnlgldi vvvitktdym stlekehdyk
      241 dehfdfmqqw irrfclqyga glfytsaked kncdllykyl thriyslpfr tpalvvekda
      301 vlipagwdnm kkisilhenl qsmkpddyyr dviaqppinr kcvakevevq aeeeqvflak
      361 qqaallglkd ptrspttrnq astgpviqva spnkkldlkg tgttqsgegv lanffnsllh
      421 kktgtpgspg sfdtgptnkg dhv
//
LOCUS       XP_034183013             437 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light intermediate chain isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183013
VERSION     XP_034183013.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327122.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..437
                     /product="dynein light intermediate chain isoform X2"
                     /calculated_mol_wt=48878
     Region          23..436
                     /region_name="DLIC"
                     /note="Dynein light intermediate chain (DLIC); pfam05783"
                     /db_xref="CDD:368612"
     CDS             1..437
                     /gene="Dlic"
                     /coded_by="XM_034327122.2:450..1763"
                     /db_xref="GeneID:117605595"
ORIGIN      
        1 matmaidnml qsngfqskkk edaendnlws silaevqnsg nnklpsnkni lvlgdnesgk
       61 ttliaklqgv edpkkgsgle fayidvrddy rddqtrlsvw vldgdpghan llrfalnaer
      121 fphtlvmlva amttpwaild qlqswaallg dhidklpldn dtrqrcrqln ikkwqdytep
      181 gdeldpgspl rrtsrnldde tidnlplpeg vlttnlgldi vvvitktdym stlekehdyk
      241 dehfdfmqqw irrfclqyga glfytsaked kncdllykyl thriyslpfr tpalvvekda
      301 vlipagwdnm kkisilhenl qsmkpddyyr dviaqppinr kcvakevevq aeeeqvflak
      361 qqaallglkd ptrspttrnq astgpviqva spnkkldlkg tgttqsgegv lanffnsllh
      421 kktgtpgspg sfgtspi
//
LOCUS       XP_034183014             435 aa            linear   INV 03-SEP-2025
DEFINITION  dynein light intermediate chain isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183014
VERSION     XP_034183014.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327123.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..435
                     /product="dynein light intermediate chain isoform X3"
                     /calculated_mol_wt=48790
     Region          23..435
                     /region_name="DLIC"
                     /note="Dynein light intermediate chain (DLIC); pfam05783"
                     /db_xref="CDD:368612"
     CDS             1..435
                     /gene="Dlic"
                     /coded_by="XM_034327123.2:446..1753"
                     /db_xref="GeneID:117605595"
ORIGIN      
        1 matmaidnml qsngfqskkk edaendnlws silaevqnsg nnklpsnkni lvlgdnesgk
       61 ttliaklqgv edpkkgsgle fayidvrddy rddqtrlsvw vldgdpghan llrfalnaer
      121 fphtlvmlva amttpwaild qlqswaallg dhidklpldn dtrqrcrqln ikkwqdytep
      181 gdeldpgspl rrtsrnldde tidnlplpeg vlttnlgldi vvvitktdym stlekehdyk
      241 dehfdfmqqw irrfclqyga glfytsaked kncdllykyl thriyslpfr tpalvvekda
      301 vlipagwdnm kkisilhenl qsmkpddyyr dviaqppinr kcvakevevq aeeeqvflak
      361 qqaallglkd ptrspttrnq vaspnkkldl kgtgttqsge gvlanffnsl lhkktgtpgs
      421 pgsfdtgptn kgdhv
//
LOCUS       XP_034183058            1015 aa            linear   INV 03-SEP-2025
DEFINITION  germ line transcription factor 1 [Osmia lignaria lignaria].
ACCESSION   XP_034183058
VERSION     XP_034183058.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327167.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1015
                     /product="germ line transcription factor 1"
                     /calculated_mol_wt=113906
     Region          261..339
                     /region_name="BRCT_RFC1"
                     /note="BRCT domain of replication factor C subunit 1
                     (RFC1) and similar proteins; cd17752"
                     /db_xref="CDD:349383"
     Site            order(273..275,282,295..299,311..317,320)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:349383"
     Region          461..>800
                     /region_name="PRK04195"
                     /note="replication factor C large subunit; Provisional"
                     /db_xref="CDD:235250"
     Region          780..933
                     /region_name="RFC1"
                     /note="Replication factor RFC1 C terminal domain;
                     pfam08519"
                     /db_xref="CDD:462507"
     CDS             1..1015
                     /gene="Gnf1"
                     /coded_by="XM_034327167.2:206..3253"
                     /db_xref="GeneID:117605634"
ORIGIN      
        1 mskdirsyfq pvkansgipn atpskakkrt vissdeddek skspikqskk sklskknvie
       61 sdsdsevvvi spsevlgktp vsrqeapkva rklqrkeakc hrdhkfedtl rqldvshiek
      121 knskenkvdk dsdlhesstn nekkkttpvk kketpekpqv ekdkeqnept kkpsnsssne
      181 diehsvpeys nkskhakkpa kgtssafnle vkriavkrka spekdvyeer vekrkhhaav
      241 ymqylqrgga rnpgskeipv gaenclagls fvvtgvldsl ereeaeelik kyggrivslp
      301 skrtnyvivg dqpgtakiak analnlktis eddllemirt rpagqsadii qprarskhrq
      361 tqkstksddm spspkkraki setketekle rsphkketse kdekkqleks shkkendskl
      421 ektpspvnke qnetktsdvk dvqvkhiard indnlsleal vekyrpktmk qilgqqgeks
      481 nakklhnwlk nwnhnqngri khskpspwak nddgaffkaa llsgppgigk tttaqvvcke
      541 ldfdlvefna sdtrskkllk eavsellsnt slkdyvttcg khnptskrvl lmdevdgmag
      601 nedrgglqel iiliknteip iicicndrnn lkmrtlanyt ydlrfqkprf eqirgamksi
      661 cfkeninist edldrliest nqdirqvinh lslfvgktat qeksekqhvn kdlklgpwdv
      721 vrkvfdaeeh knmsihdksd lffhdysfap lfiqdnylmv tphaprnqtl drvakcaesl
      781 algdiveksi rsnnnwsllp vqacyssvip gtvmsghigg qinfpawlgr nskankfdrl
      841 iqeltihmrl ttgaskegvn ldylkplrna itrplvtagm egvdaaidim nkyhllredl
      901 nslievslwp gdrdpmeaid skvkgaftkt ynknaapvpy ivsgiskkka tsalqdeaal
      961 veeeeeevan sdddtietdk mikakkpttt kkteskaknt snqpskrgrg rgkak
//
LOCUS       XP_034183059             839 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605635 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183059
VERSION     XP_034183059.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327168.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183059.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..839
                     /product="uncharacterized protein LOC117605635 isoform X1"
                     /calculated_mol_wt=95471
     Region          151..310
                     /region_name="PXA"
                     /note="PXA domain; pfam02194"
                     /db_xref="CDD:460484"
     Region          461..>527
                     /region_name="PX_domain"
                     /note="The Phox Homology domain, a phosphoinositide
                     binding module; cl02563"
                     /db_xref="CDD:470617"
     Region          <502..563
                     /region_name="PX_domain"
                     /note="The Phox Homology domain, a phosphoinositide
                     binding module; cl02563"
                     /db_xref="CDD:470617"
     CDS             1..839
                     /gene="LOC117605635"
                     /coded_by="XM_034327168.2:721..3240"
                     /db_xref="GeneID:117605635"
ORIGIN      
        1 mgkiipmswl nqillilsil cvslsliiqq wagliplpiy isiswaifii imsvwlscfl
       61 lqltlktnsp lkikllhnwl yqklanslkl hkpekkddsn sesenkakss ninfvqitne
      121 kktgnvskik klhdktvksg aksislrkkk neelnnlvke ihlkcinawy ksisneksfp
      181 teaqellrkl ltklfykiss idkvvlthkl snvfllhlke yyralrrvek gtapnieeaf
      241 kylhpgsrnt stlehmlhqm vtilseeflq weltsslpck illsilakrl llvietissp
      301 nwlfqslsdl lqpipkeaae vlnnqgadtl htkkiisnal sdgmasataa viprplpkqk
      361 sdsstkpssr dknsvstndq rpilhldnlt keyrglwgqs mteldsevde dkmspvyeep
      421 tdfattiarl rnvlqqkstv ntplhveeks yvvyegsqfi nlsipwtefh tafdgsqqll
      481 yciqfddveq revdlfettt atvrrqyrdf aqlhtslqei pelapimqdl vlpeggrvel
      541 esylktltrr lanecpsqlr hflrpsssag kkadavaprf drflaktvtg vfntlrtvvp
      601 gfeieqeeet vplptlmpls dipwrfveni kskslltelq qliterteys svdtayeavd
      661 smegtgnsal lshwweivkn syeeemdeld shlmltciav dlicelltgv gstntlqqea
      721 vvrwtkllfg nvtepilqta ilrvfdylgn mslyrniqnk iseepltelk ekllqqlltk
      781 ipsdiklifg eddmlnilky lldsyeikki nldlnlqivd vlasqllisc rlnhdgaaf
//
LOCUS       XP_034183060             838 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605635 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183060
VERSION     XP_034183060.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327169.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183060.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..838
                     /product="uncharacterized protein LOC117605635 isoform X2"
                     /calculated_mol_wt=95342
     Region          151..310
                     /region_name="PXA"
                     /note="PXA domain; pfam02194"
                     /db_xref="CDD:460484"
     Region          461..>527
                     /region_name="PX_domain"
                     /note="The Phox Homology domain, a phosphoinositide
                     binding module; cl02563"
                     /db_xref="CDD:470617"
     Region          <502..563
                     /region_name="PX_domain"
                     /note="The Phox Homology domain, a phosphoinositide
                     binding module; cl02563"
                     /db_xref="CDD:470617"
     CDS             1..838
                     /gene="LOC117605635"
                     /coded_by="XM_034327169.2:724..3240"
                     /db_xref="GeneID:117605635"
ORIGIN      
        1 mgkiipmswl nqillilsil cvslsliiqq wagliplpiy isiswaifii imsvwlscfl
       61 lqltlktnsp lkikllhnwl yqklanslkl hkpekkddsn sesenkakss ninfvqitne
      121 kktgnvskik klhdktvksg aksislrkkk neelnnlvke ihlkcinawy ksisneksfp
      181 teaqellrkl ltklfykiss idkvvlthkl snvfllhlke yyralrrvek gtapnieeaf
      241 kylhpgsrnt stlehmlhqm vtilseeflq weltsslpck illsilakrl llvietissp
      301 nwlfqslsdl lqpipkeaae vlnnqgadtl htkkiisnal sdgmasataa viprplpkqk
      361 sdsstkpssr dknsvstndq rpilhldnlt keyrglwgqs mteldsevde dkmspvyeep
      421 tdfattiarl rnvlqqkstv ntplhveeks yvvyegsqfi nlsipwtefh tafdgsqqll
      481 yciqfddveq revdlfettt atvrrqyrdf aqlhtslqei pelapimqdl vlpeggrvel
      541 esylktltrr lanecpsqlr hflrpsssag kkadavaprf drflaktvtg vfntlrtvvp
      601 gfeieqeeet vplptlmpls dipwrfveni kskslltelq qliterteys svdtayeavd
      661 smegtgnsal lshwweivkn syeemdelds hlmltciavd licelltgvg stntlqqeav
      721 vrwtkllfgn vtepilqtai lrvfdylgnm slyrniqnki seepltelke kllqqlltki
      781 psdiklifge ddmlnilkyl ldsyeikkin ldlnlqivdv lasqlliscr lnhdgaaf
//
LOCUS       XP_034183062             645 aa            linear   INV 03-SEP-2025
DEFINITION  angiotensin-converting enzyme-like [Osmia lignaria lignaria].
ACCESSION   XP_034183062
VERSION     XP_034183062.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327171.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..645
                     /product="angiotensin-converting enzyme-like"
                     /calculated_mol_wt=74484
     Region          38..619
                     /region_name="Peptidase_M2"
                     /note="Angiotensin-converting enzyme; pfam01401"
                     /db_xref="CDD:460196"
     CDS             1..645
                     /gene="LOC117605636"
                     /coded_by="XM_034327171.2:169..2106"
                     /db_xref="GeneID:117605636"
ORIGIN      
        1 mlrlllvaal vtlslgvpap qndgtevaeg sddlqkrate flsrvdklya ewnnkqtlad
       61 weyasnltpg nlakklnvss eaarvtksiw kevnefpwrd ikdenirrqf sklsvlgtaa
      121 lpedlfqeye kiisdmeniy atakicdykd rskcnlalep elteilmksr dpeelkyiwv
      181 qwrkatgekm kslykryvel snmaatlnnf tdnaaywmkd yeaddfpeqi dalwqqlkpl
      241 ylqlhayvrr elrkkygedi vskdgpipah llgnmwaqaw sniadftipy pgkqlpdvtd
      301 amvkqgynat tifrvaedff tsinltampd lfwersilek pkdrelicha sawdfydgkd
      361 frikqctrvn tedlltahhe mghveyylqy knqptvykeg anpgfheavg dvialsastp
      421 shlkkinlle ddstdreani nhlylkgldk iiflpfaymm dkwrwnvfqg kvttdnyncn
      481 wwdlaevfqg ieppvdrsed dfdpgakyhi vadveyiryy vsfvvqfqfh kslcieakqy
      541 dpqnpdskpl hqcdiynnka agnllksmle lgsskpwpda mekitgqrnm dsaglleyfk
      601 pltdwlteen kktneyigwk srakqcvqtr selapettet tdaie
//
LOCUS       XP_034183063             376 aa            linear   INV 03-SEP-2025
DEFINITION  actin, muscle [Osmia lignaria lignaria].
ACCESSION   XP_034183063
VERSION     XP_034183063.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327172.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="actin, muscle"
                     /calculated_mol_wt=41627
     Region          7..371
                     /region_name="ASKHA_NBD_actin"
                     /note="nucleotide-binding domain (NBD) of actin and
                     similar proteins; cd10224"
                     /db_xref="CDD:466823"
     CDS             1..376
                     /gene="LOC117605637"
                     /coded_by="XM_034327172.2:237..1367"
                     /db_xref="GeneID:117605637"
ORIGIN      
        1 mcdddvaalv vdngsgmcka gfagddapra vfpsivgrpr hqgvmvgmgq kdsyvgdeaq
       61 skrgiltlky piehgiitnw ddmekiwhht fynelrvape ehpvllteap lnpkanrekm
      121 tqimfetfns pamyvaiqav lslyasgrtt givldsgdgv shtvpiyegy alphailrld
      181 lagrdltdyl mkiltergys ftttaereiv rdikeklcyv aldfeqemat aaastsleks
      241 yelpdgqvit ignerfrcpe alfqpsflgm escgihetvy nsimkcdvdi rkdlyantvl
      301 sggttmypgi adrmqkeita lapstikiki iapperkysv wiggsilasl stfqqmwisk
      361 qeydesgpgi vhrkcf
//
LOCUS       XP_034183064             376 aa            linear   INV 03-SEP-2025
DEFINITION  actin, clone 205 [Osmia lignaria lignaria].
ACCESSION   XP_034183064
VERSION     XP_034183064.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327173.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="actin, clone 205"
                     /calculated_mol_wt=41654
     Region          7..371
                     /region_name="ASKHA_NBD_actin"
                     /note="nucleotide-binding domain (NBD) of actin and
                     similar proteins; cd10224"
                     /db_xref="CDD:466823"
     CDS             1..376
                     /gene="LOC117605638"
                     /coded_by="XM_034327173.2:182..1312"
                     /db_xref="GeneID:117605638"
ORIGIN      
        1 mcddevaalv vdngsgmcka gfagddapra vfpsivgrpr hqgvmvgmgq kdsyvgdeaq
       61 skrgiltlky piehgiitnw ddmekiwhht fynelrvape ehpvllteap lnpkanrekm
      121 tqimfetfns pamyvaiqav lslyasgrtt givldsgdgv shtvpiyegy alphailrld
      181 lagrdltdyl mkiltergys ftttaereiv rdikeklcyv aldfeqemat aaastsleks
      241 yelpdgqvit ignerfrcpe alfqpsflgm escgihetvy nsimkcdvdi rkdlyannvl
      301 sggttmypgi adrmqkeita lapstikiki iapperkysv wiggsilasl stfqqmwisk
      361 qeydesgpgi vhrkcf
//
LOCUS       XP_034183066             376 aa            linear   INV 03-SEP-2025
DEFINITION  actin, clone 205 [Osmia lignaria lignaria].
ACCESSION   XP_034183066
VERSION     XP_034183066.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327175.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..376
                     /product="actin, clone 205"
                     /calculated_mol_wt=41654
     Region          7..371
                     /region_name="ASKHA_NBD_actin"
                     /note="nucleotide-binding domain (NBD) of actin and
                     similar proteins; cd10224"
                     /db_xref="CDD:466823"
     CDS             1..376
                     /gene="LOC117605639"
                     /coded_by="XM_034327175.2:180..1310"
                     /db_xref="GeneID:117605639"
ORIGIN      
        1 mcddevaalv vdngsgmcka gfagddapra vfpsivgrpr hqgvmvgmgq kdsyvgdeaq
       61 skrgiltlky piehgiitnw ddmekiwhht fynelrvape ehpvllteap lnpkanrekm
      121 tqimfetfns pamyvaiqav lslyasgrtt givldsgdgv shtvpiyegy alphailrld
      181 lagrdltdyl mkiltergys ftttaereiv rdikeklcyv aldfeqemat aaastsleks
      241 yelpdgqvit ignerfrcpe alfqpsflgm escgihetvy nsimkcdvdi rkdlyannvl
      301 sggttmypgi adrmqkeita lapstikiki iapperkysv wiggsilasl stfqqmwisk
      361 qeydesgpgi vhrkcf
//
LOCUS       XP_034183067             220 aa            linear   INV 03-SEP-2025
DEFINITION  sugar transporter SWEET1 [Osmia lignaria lignaria].
ACCESSION   XP_034183067
VERSION     XP_034183067.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327176.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..220
                     /product="sugar transporter SWEET1"
                     /calculated_mol_wt=24485
     Region          14..90
                     /region_name="MtN3_slv"
                     /note="Sugar efflux transporter for intercellular
                     exchange; cl46784"
                     /db_xref="CDD:481124"
     Region          125..208
                     /region_name="MtN3_slv"
                     /note="Sugar efflux transporter for intercellular
                     exchange; cl46784"
                     /db_xref="CDD:481124"
     CDS             1..220
                     /gene="LOC117605640"
                     /coded_by="XM_034327176.2:416..1078"
                     /db_xref="GeneID:117605640"
ORIGIN      
        1 mgledykeiv atcatitsmg qmlsgtfmck diykkgsskg vdpmpflggi gmcilmlqya
       61 wivkdpgmin invfgvlint lymaiyyyyt shtkdtlali gktaafvmvf lvyaqmenpe
      121 kieyrfgliv ttlflllias plihlgevir tqntdvlpfp lifmgtlasf qwllygliin
      181 nvfiifqnti gfllsvvqms lfvifpsksk akatgqektd
//
LOCUS       XP_034183068              76 aa            linear   INV 03-SEP-2025
DEFINITION  suppressor of exocyst mutations 1 [Osmia lignaria lignaria].
ACCESSION   XP_034183068
VERSION     XP_034183068.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327177.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..76
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..76
                     /product="suppressor of exocyst mutations 1"
                     /calculated_mol_wt=8850
     CDS             1..76
                     /gene="Sem1"
                     /coded_by="XM_034327177.2:80..310"
                     /db_xref="GeneID:117605641"
ORIGIN      
        1 mtdnkndktk vdlglleedd efeefpaedw tavdednedi svwednwddd dveddfnqql
       61 raqlekqkaq kqgkes
//
LOCUS       XP_034183112            1091 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183112
VERSION     XP_034183112.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327221.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183112.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1091
                     /product="uncharacterized protein LOC117605661 isoform X1"
                     /calculated_mol_wt=120369
     Region          <136..>388
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1091
                     /gene="LOC117605661"
                     /coded_by="XM_034327221.2:410..3685"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mlgvlkrrwk pskrassvrg pdsplsselt ldkprpipsp rqihplygek agllgycdpv
       61 gntenfppap nivveggrie fvrpsqdgtt tprrntmsrg sqtfsdesek sdpakvrsae
      121 wcignpllss racineslee rvqeleqalq aerfaaqrdr atitklqrqi nkreatqrdv
      181 ererrqrmea earvreatae aerarsrvhh lqrelarmee tsrgllqhkh raeqlkqekt
      241 altlsyearv hqyqaqiskl qlenesmrgq lrgleaacag evhaalvarl atletehaal
      301 ardadaqrrq yerclddvan qvvrallsqk glreeigalq rrireleaqn rtlsgllaqa
      361 aspgspteli qgileagpaa vvaalgldpa wvplarprsl nlqipvmaat kcrrnrplaq
      421 kpseeegses pesgnrdegy stmssdvqge garqepsrgl edlkeatdet eadvspdarl
      481 valdagdpdv lflplnltvg inprhsyppt kdllpyqhvm rsfsdshlcl klttttnftp
      541 yklsspigss slllveeegw daeyvqqwlr lddsrsaqqh rdlleleydr aeledwsfsl
      601 stedlvqnes tvwkepattt ttpalpaike hnpleleeda neclwngasy ladrtggelv
      661 allmdaratg swpyatspga swsseegale nsskrssaal sgcsddpdsp svgtdftrdf
      721 yrlvkfestk slastssrsg rpppdreqal qsvlsfiaeq qmylaelpnn llehgnvpla
      781 tevveetenk dergteamct eietsvdqdn sntenvvets snddlldqip vpslapeeri
      841 isavacnggv syttvapsel gavpeedeea atsstpstvv sparapllve grdrslsfhe
      901 ratskdvide lnrmirkgee htvthesqvp lekldlaccc ptgwvhverd idftdpkara
      961 nlldvmless esssatsssg sagsdsgdep pdyrhlhrlh ryrrqkkasa aqahrvlrlp
     1021 stwgssrpsi igrgedffvr ygdkereava sfdfldeivp pssrgsntsl idlddtpsae
     1081 lrgdimklsp l
//
LOCUS       XP_034183118            1083 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183118
VERSION     XP_034183118.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327227.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183118.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1083
                     /product="uncharacterized protein LOC117605661 isoform X2"
                     /calculated_mol_wt=119473
     Region          <136..>380
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1083
                     /gene="LOC117605661"
                     /coded_by="XM_034327227.2:410..3661"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mlgvlkrrwk pskrassvrg pdsplsselt ldkprpipsp rqihplygek agllgycdpv
       61 gntenfppap nivveggrie fvrpsqdgtt tprrntmsrg sqtfsdesek sdpakvrsae
      121 wcignpllss racineslee rvqeleqalq aerfaaqrdr atitklqrqi nkreatqrdv
      181 ererrqrmea earvreatae aerarsrvhh lqrelarmee tsrgllqhkh raeqlkqekt
      241 altlsyearv hqyqaqiskl qlenesmrgq lrgleaacag evhaalvarl atletehaal
      301 ardadaqrrq yerclddvan qvglreeiga lqrrirelea qnrtlsglla qaaspgspte
      361 liqgileagp aavvaalgld pawvplarpr slnlqipvma atkcrrnrpl aqkpseeegs
      421 espesgnrde gystmssdvq gegarqepsr gledlkeatd eteadvspda rlvaldagdp
      481 dvlflplnlt vginprhsyp ptkdllpyqh vmrsfsdshl clklttttnf tpyklsspig
      541 ssslllveee gwdaeyvqqw lrlddsrsaq qhrdlleley draeledwsf slstedlvqn
      601 estvwkepat ttttpalpai kehnplelee daneclwnga syladrtgge lvallmdara
      661 tgswpyatsp gaswsseega lensskrssa alsgcsddpd spsvgtdftr dfyrlvkfes
      721 tkslastssr sgrpppdreq alqsvlsfia eqqmylaelp nnllehgnvp latevveete
      781 nkdergteam cteietsvdq dnsntenvve tssnddlldq ipvpslapee riisavacng
      841 gvsyttvaps elgavpeede eaatsstpst vvsparapll vegrdrslsf heratskdvi
      901 delnrmirkg eehtvthesq vplekldlac ccptgwvhve rdidftdpka ranlldvmle
      961 ssesssatss sgsagsdsgd eppdyrhlhr lhryrrqkka saaqahrvlr lpstwgssrp
     1021 siigrgedff vrygdkerea vasfdfldei vppssrgsnt slidlddtps aelrgdimkl
     1081 spl
//
LOCUS       XP_034183119            1082 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183119
VERSION     XP_034183119.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327228.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183119.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1082
                     /product="uncharacterized protein LOC117605661 isoform X3"
                     /calculated_mol_wt=119374
     Region          <136..>379
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1082
                     /gene="LOC117605661"
                     /coded_by="XM_034327228.2:410..3658"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mlgvlkrrwk pskrassvrg pdsplsselt ldkprpipsp rqihplygek agllgycdpv
       61 gntenfppap nivveggrie fvrpsqdgtt tprrntmsrg sqtfsdesek sdpakvrsae
      121 wcignpllss racineslee rvqeleqalq aerfaaqrdr atitklqrqi nkreatqrdv
      181 ererrqrmea earvreatae aerarsrvhh lqrelarmee tsrgllqhkh raeqlkqekt
      241 altlsyearv hqyqaqiskl qlenesmrgq lrgleaacag evhaalvarl atletehaal
      301 ardadaqrrq yerclddvan qglreeigal qrrireleaq nrtlsgllaq aaspgsptel
      361 iqgileagpa avvaalgldp awvplarprs lnlqipvmaa tkcrrnrpla qkpseeegse
      421 spesgnrdeg ystmssdvqg egarqepsrg ledlkeatde teadvspdar lvaldagdpd
      481 vlflplnltv ginprhsypp tkdllpyqhv mrsfsdshlc lklttttnft pyklsspigs
      541 sslllveeeg wdaeyvqqwl rlddsrsaqq hrdlleleyd raeledwsfs lstedlvqne
      601 stvwkepatt tttpalpaik ehnpleleed aneclwngas yladrtggel vallmdarat
      661 gswpyatspg aswsseegal ensskrssaa lsgcsddpds psvgtdftrd fyrlvkfest
      721 kslastssrs grpppdreqa lqsvlsfiae qqmylaelpn nllehgnvpl atevveeten
      781 kdergteamc teietsvdqd nsntenvvet ssnddlldqi pvpslapeer iisavacngg
      841 vsyttvapse lgavpeedee aatsstpstv vsparapllv egrdrslsfh eratskdvid
      901 elnrmirkge ehtvthesqv plekldlacc cptgwvhver didftdpkar anlldvmles
      961 sesssatsss gsagsdsgde ppdyrhlhrl hryrrqkkas aaqahrvlrl pstwgssrps
     1021 iigrgedffv rygdkereav asfdfldeiv ppssrgsnts lidlddtpsa elrgdimkls
     1081 pl
//
LOCUS       XP_034183120            1071 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183120
VERSION     XP_034183120.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327229.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183120.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1071
                     /product="uncharacterized protein LOC117605661 isoform X4"
                     /calculated_mol_wt=118198
     Region          <115..>368
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1071
                     /gene="LOC117605661"
                     /coded_by="XM_034327229.2:409..3624"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mlgvlkrrwk pskrassvrg pdsplsselt ldkprpipsp rqihplygek agllgycdpv
       61 gntenfppap nivveggrie fvrpsqdgtt tprrntmsrg sqtfsdesek sdpakeslee
      121 rvqeleqalq aerfaaqrdr atitklqrqi nkreatqrdv ererrqrmea earvreatae
      181 aerarsrvhh lqrelarmee tsrgllqhkh raeqlkqekt altlsyearv hqyqaqiskl
      241 qlenesmrgq lrgleaacag evhaalvarl atletehaal ardadaqrrq yerclddvan
      301 qvvrallsqk glreeigalq rrireleaqn rtlsgllaqa aspgspteli qgileagpaa
      361 vvaalgldpa wvplarprsl nlqipvmaat kcrrnrplaq kpseeegses pesgnrdegy
      421 stmssdvqge garqepsrgl edlkeatdet eadvspdarl valdagdpdv lflplnltvg
      481 inprhsyppt kdllpyqhvm rsfsdshlcl klttttnftp yklsspigss slllveeegw
      541 daeyvqqwlr lddsrsaqqh rdlleleydr aeledwsfsl stedlvqnes tvwkepattt
      601 ttpalpaike hnpleleeda neclwngasy ladrtggelv allmdaratg swpyatspga
      661 swsseegale nsskrssaal sgcsddpdsp svgtdftrdf yrlvkfestk slastssrsg
      721 rpppdreqal qsvlsfiaeq qmylaelpnn llehgnvpla tevveetenk dergteamct
      781 eietsvdqdn sntenvvets snddlldqip vpslapeeri isavacnggv syttvapsel
      841 gavpeedeea atsstpstvv sparapllve grdrslsfhe ratskdvide lnrmirkgee
      901 htvthesqvp lekldlaccc ptgwvhverd idftdpkara nlldvmless esssatsssg
      961 sagsdsgdep pdyrhlhrlh ryrrqkkasa aqahrvlrlp stwgssrpsi igrgedffvr
     1021 ygdkereava sfdfldeivp pssrgsntsl idlddtpsae lrgdimklsp l
//
LOCUS       XP_034183121            1033 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183121
VERSION     XP_034183121.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327230.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183121.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1033
                     /product="uncharacterized protein LOC117605661 isoform X5"
                     /calculated_mol_wt=114175
     Region          <136..>388
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1033
                     /gene="LOC117605661"
                     /coded_by="XM_034327230.2:410..3511"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mlgvlkrrwk pskrassvrg pdsplsselt ldkprpipsp rqihplygek agllgycdpv
       61 gntenfppap nivveggrie fvrpsqdgtt tprrntmsrg sqtfsdesek sdpakvrsae
      121 wcignpllss racineslee rvqeleqalq aerfaaqrdr atitklqrqi nkreatqrdv
      181 ererrqrmea earvreatae aerarsrvhh lqrelarmee tsrgllqhkh raeqlkqekt
      241 altlsyearv hqyqaqiskl qlenesmrgq lrgleaacag evhaalvarl atletehaal
      301 ardadaqrrq yerclddvan qvvrallsqk glreeigalq rrireleaqn rtlsgllaqa
      361 aspgspteli qgileagpaa vvaalgldpa wvplarprsl nlqipvmaat kcrrnrplaq
      421 kpseeegses pesgnrdegy stmssdvqge garqepsrgl edlkeatdet eadvspdarl
      481 valdagdpdv lflplnltvg inprhsyppt kdllpyqhvm rsfsdshlcl klttttnftp
      541 yklsspigss slllveeegw daeyvqqwlr lddsrsaqqh rdlleleydr aeledwsfsl
      601 stedlvqnes tvwkepattt ttpalpaike hnpleleeda neclwngasy ladrtggelv
      661 allmdaratg swpyatspga swsseegale nsskrssaal sgcsddpdsp svgtdftrdf
      721 yrlvkfestk slastssrsg rpppdreqal qsvlsfiaeq qmylaelpnn llehgnvpla
      781 tevveetenk dergteamct eietsvdqdn sntenvvets snddlldqip vpslapeeri
      841 isavacnggv syttvapsel gavpeedeea atsstpstvv sparapllve grdrslsfhe
      901 ratskdvide lnrmirkgee htvthesqvp lekldlaccc ptgwvhverd idftdpkara
      961 nlldvmless esssatsssg sagsdsgdep pdyrhlhrlh ryrrqkkdgs syavcfdchk
     1021 ksyvvrlsdd qfl
//
LOCUS       XP_034183122            1010 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183122
VERSION     XP_034183122.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327231.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183122.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1010
                     /product="uncharacterized protein LOC117605661 isoform X6"
                     /calculated_mol_wt=111535
     Region          <136..>388
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1010
                     /gene="LOC117605661"
                     /coded_by="XM_034327231.2:410..3442"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mlgvlkrrwk pskrassvrg pdsplsselt ldkprpipsp rqihplygek agllgycdpv
       61 gntenfppap nivveggrie fvrpsqdgtt tprrntmsrg sqtfsdesek sdpakvrsae
      121 wcignpllss racineslee rvqeleqalq aerfaaqrdr atitklqrqi nkreatqrdv
      181 ererrqrmea earvreatae aerarsrvhh lqrelarmee tsrgllqhkh raeqlkqekt
      241 altlsyearv hqyqaqiskl qlenesmrgq lrgleaacag evhaalvarl atletehaal
      301 ardadaqrrq yerclddvan qvvrallsqk glreeigalq rrireleaqn rtlsgllaqa
      361 aspgspteli qgileagpaa vvaalgldpa wvplarprsl nlqipvmaat kcrrnrplaq
      421 kpseeegses pesgnrdegy stmssdvqge garqepsrgl edlkeatdet eadvspdarl
      481 valdagdpdv lflplnltvg inprhsyppt kdllpyqhvm rsfsdshlcl klttttnftp
      541 yklsspigss slllveeegw daeyvqqwlr lddsrsaqqh rdlleleydr aeledwsfsl
      601 stedlvqnes tvwkepattt ttpalpaike hnpleleeda neclwngasy ladrtggelv
      661 allmdaratg swpyatspga swsseegale nsskrssaal sgcsddpdsp svgtdftrdf
      721 yrlvkfestk slastssrsg rpppdreqal qsvlsfiaeq qmylaelpnn llehgnvpla
      781 tevveetenk dergteamct eietsvdqdn sntenvvets snddlldqip vpslapeeri
      841 isavacnggv syttvapsel gavpeedeea atsstpstvv sparapllve grdrslsfhe
      901 ratskdvide lnrmirkgee htvthesqvp lekldlaccc ptgwvhverd idftdpkara
      961 nlldvmless esssatsssg sagsdsgdep pdyrhlhrlh ryrrqkkepv
//
LOCUS       XP_034183123            1008 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183123
VERSION     XP_034183123.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327232.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183123.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1008
                     /product="uncharacterized protein LOC117605661 isoform X7"
                     /calculated_mol_wt=111325
     Region          <136..>388
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1008
                     /gene="LOC117605661"
                     /coded_by="XM_034327232.2:410..3436"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mlgvlkrrwk pskrassvrg pdsplsselt ldkprpipsp rqihplygek agllgycdpv
       61 gntenfppap nivveggrie fvrpsqdgtt tprrntmsrg sqtfsdesek sdpakvrsae
      121 wcignpllss racineslee rvqeleqalq aerfaaqrdr atitklqrqi nkreatqrdv
      181 ererrqrmea earvreatae aerarsrvhh lqrelarmee tsrgllqhkh raeqlkqekt
      241 altlsyearv hqyqaqiskl qlenesmrgq lrgleaacag evhaalvarl atletehaal
      301 ardadaqrrq yerclddvan qvvrallsqk glreeigalq rrireleaqn rtlsgllaqa
      361 aspgspteli qgileagpaa vvaalgldpa wvplarprsl nlqipvmaat kcrrnrplaq
      421 kpseeegses pesgnrdegy stmssdvqge garqepsrgl edlkeatdet eadvspdarl
      481 valdagdpdv lflplnltvg inprhsyppt kdllpyqhvm rsfsdshlcl klttttnftp
      541 yklsspigss slllveeegw daeyvqqwlr lddsrsaqqh rdlleleydr aeledwsfsl
      601 stedlvqnes tvwkepattt ttpalpaike hnpleleeda neclwngasy ladrtggelv
      661 allmdaratg swpyatspga swsseegale nsskrssaal sgcsddpdsp svgtdftrdf
      721 yrlvkfestk slastssrsg rpppdreqal qsvlsfiaeq qmylaelpnn llehgnvpla
      781 tevveetenk dergteamct eietsvdqdn sntenvvets snddlldqip vpslapeeri
      841 isavacnggv syttvapsel gavpeedeea atsstpstvv sparapllve grdrslsfhe
      901 ratskdvide lnrmirkgee htvthesqvp lekldlaccc ptgwvhverd idftdpkara
      961 nlldvmless esssatsssg sagsdsgdep pdyrhlhrlh ryrrqkkd
//
LOCUS       XP_034183124            1000 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183124
VERSION     XP_034183124.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327233.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183124.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1000
                     /product="uncharacterized protein LOC117605661 isoform X8"
                     /calculated_mol_wt=110866
     Region          <45..>297
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..1000
                     /gene="LOC117605661"
                     /coded_by="XM_034327233.2:8..3010"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 meqvlcrslv rsfvyisssl svyirreaqf rsshrvvwmi syliesleer vqeleqalqa
       61 erfaaqrdra titklqrqin kreatqrdve rerrqrmeae arvreataea erarsrvhhl
      121 qrelarmeet srgllqhkhr aeqlkqekta ltlsyearvh qyqaqisklq lenesmrgql
      181 rgleaacage vhaalvarla tletehaala rdadaqrrqy erclddvanq vvrallsqkg
      241 lreeigalqr rireleaqnr tlsgllaqaa spgspteliq gileagpaav vaalgldpaw
      301 vplarprsln lqipvmaatk crrnrplaqk pseeegsesp esgnrdegys tmssdvqgeg
      361 arqepsrgle dlkeatdete advspdarlv aldagdpdvl flplnltvgi nprhsypptk
      421 dllpyqhvmr sfsdshlclk lttttnftpy klsspigsss lllveeegwd aeyvqqwlrl
      481 ddsrsaqqhr dlleleydra eledwsfsls tedlvqnest vwkepatttt tpalpaikeh
      541 npleleedan eclwngasyl adrtggelva llmdaratgs wpyatspgas wsseegalen
      601 sskrssaals gcsddpdsps vgtdftrdfy rlvkfestks lastssrsgr pppdreqalq
      661 svlsfiaeqq mylaelpnnl lehgnvplat evveetenkd ergteamcte ietsvdqdns
      721 ntenvvetss nddlldqipv pslapeerii savacnggvs yttvapselg avpeedeeaa
      781 tsstpstvvs parapllveg rdrslsfher atskdvidel nrmirkgeeh tvthesqvpl
      841 ekldlacccp tgwvhverdi dftdpkaran lldvmlesse sssatsssgs agsdsgdepp
      901 dyrhlhrlhr yrrqkkasaa qahrvlrlps twgssrpsii grgedffvry gdkereavas
      961 fdfldeivpp ssrgsntsli dlddtpsael rgdimklspl
//
LOCUS       XP_034183127             995 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183127
VERSION     XP_034183127.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327236.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183127.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..995
                     /product="uncharacterized protein LOC117605661 isoform X9"
                     /calculated_mol_wt=109827
     Region          <40..>292
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..995
                     /gene="LOC117605661"
                     /coded_by="XM_034327236.2:726..3713"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 msrgsqtfsd eseksdpakv rsaewcignp llssracine sleervqele qalqaerfaa
       61 qrdratitkl qrqinkreat qrdvererrq rmeaearvre ataeaerars rvhhlqrela
      121 rmeetsrgll qhkhraeqlk qektaltlsy earvhqyqaq isklqlenes mrgqlrglea
      181 acagevhaal varlatlete haalardada qrrqyercld dvanqvvral lsqkglreei
      241 galqrrirel eaqnrtlsgl laqaaspgsp teliqgilea gpaavvaalg ldpawvplar
      301 prslnlqipv maatkcrrnr plaqkpseee gsespesgnr degystmssd vqgegarqep
      361 srgledlkea tdeteadvsp darlvaldag dpdvlflpln ltvginprhs ypptkdllpy
      421 qhvmrsfsds hlclkltttt nftpyklssp igssslllve eegwdaeyvq qwlrlddsrs
      481 aqqhrdllel eydraeledw sfslstedlv qnestvwkep atttttpalp aikehnplel
      541 eedaneclwn gasyladrtg gelvallmda ratgswpyat spgaswssee galensskrs
      601 saalsgcsdd pdspsvgtdf trdfyrlvkf estkslasts srsgrpppdr eqalqsvlsf
      661 iaeqqmylae lpnnllehgn vplatevvee tenkdergte amcteietsv dqdnsntenv
      721 vetssnddll dqipvpslap eeriisavac nggvsyttva pselgavpee deeaatsstp
      781 stvvsparap llvegrdrsl sfheratskd videlnrmir kgeehtvthe sqvplekldl
      841 acccptgwvh verdidftdp karanlldvm lessesssat sssgsagsds gdeppdyrhl
      901 hrlhryrrqk kasaaqahrv lrlpstwgss rpsiigrged ffvrygdker eavasfdfld
      961 eivppssrgs ntslidlddt psaelrgdim klspl
//
LOCUS       XP_034183128             995 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183128
VERSION     XP_034183128.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327237.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183128.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..995
                     /product="uncharacterized protein LOC117605661 isoform X9"
                     /calculated_mol_wt=109827
     Region          <40..>292
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..995
                     /gene="LOC117605661"
                     /coded_by="XM_034327237.2:380..3367"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 msrgsqtfsd eseksdpakv rsaewcignp llssracine sleervqele qalqaerfaa
       61 qrdratitkl qrqinkreat qrdvererrq rmeaearvre ataeaerars rvhhlqrela
      121 rmeetsrgll qhkhraeqlk qektaltlsy earvhqyqaq isklqlenes mrgqlrglea
      181 acagevhaal varlatlete haalardada qrrqyercld dvanqvvral lsqkglreei
      241 galqrrirel eaqnrtlsgl laqaaspgsp teliqgilea gpaavvaalg ldpawvplar
      301 prslnlqipv maatkcrrnr plaqkpseee gsespesgnr degystmssd vqgegarqep
      361 srgledlkea tdeteadvsp darlvaldag dpdvlflpln ltvginprhs ypptkdllpy
      421 qhvmrsfsds hlclkltttt nftpyklssp igssslllve eegwdaeyvq qwlrlddsrs
      481 aqqhrdllel eydraeledw sfslstedlv qnestvwkep atttttpalp aikehnplel
      541 eedaneclwn gasyladrtg gelvallmda ratgswpyat spgaswssee galensskrs
      601 saalsgcsdd pdspsvgtdf trdfyrlvkf estkslasts srsgrpppdr eqalqsvlsf
      661 iaeqqmylae lpnnllehgn vplatevvee tenkdergte amcteietsv dqdnsntenv
      721 vetssnddll dqipvpslap eeriisavac nggvsyttva pselgavpee deeaatsstp
      781 stvvsparap llvegrdrsl sfheratskd videlnrmir kgeehtvthe sqvplekldl
      841 acccptgwvh verdidftdp karanlldvm lessesssat sssgsagsds gdeppdyrhl
      901 hrlhryrrqk kasaaqahrv lrlpstwgss rpsiigrged ffvrygdker eavasfdfld
      961 eivppssrgs ntslidlddt psaelrgdim klspl
//
LOCUS       XP_034183129             929 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117605661 isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_034183129
VERSION     XP_034183129.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327238.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183129.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..929
                     /product="uncharacterized protein LOC117605661 isoform
                     X10"
                     /calculated_mol_wt=102294
     Region          <8..>199
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     CDS             1..929
                     /gene="LOC117605661"
                     /coded_by="XM_034327238.2:1349..4138"
                     /db_xref="GeneID:117605661"
ORIGIN      
        1 mganltkqsr reatqrdver errqrmeaea rvreataeae rarsrvhhlq relarmeets
       61 rgllqhkhra eqlkqektal tlsyearvhq yqaqisklql enesmrgqlr gleaacagev
      121 haalvarlat letehaalar dadaqrrqye rclddvanqv vrallsqkgl reeigalqrr
      181 ireleaqnrt lsgllaqaas pgspteliqg ileagpaavv aalgldpawv plarprslnl
      241 qipvmaatkc rrnrplaqkp seeegsespe sgnrdegyst mssdvqgega rqepsrgled
      301 lkeatdetea dvspdarlva ldagdpdvlf lplnltvgin prhsypptkd llpyqhvmrs
      361 fsdshlclkl ttttnftpyk lsspigsssl llveeegwda eyvqqwlrld dsrsaqqhrd
      421 lleleydrae ledwsfslst edlvqnestv wkepattttt palpaikehn pleleedane
      481 clwngasyla drtggelval lmdaratgsw pyatspgasw sseegalens skrssaalsg
      541 csddpdspsv gtdftrdfyr lvkfestksl astssrsgrp ppdreqalqs vlsfiaeqqm
      601 ylaelpnnll ehgnvplate vveetenkde rgteamctei etsvdqdnsn tenvvetssn
      661 ddlldqipvp slapeeriis avacnggvsy ttvapselga vpeedeeaat sstpstvvsp
      721 arapllvegr drslsfhera tskdvideln rmirkgeeht vthesqvple kldlacccpt
      781 gwvhverdid ftdpkaranl ldvmlesses ssatsssgsa gsdsgdeppd yrhlhrlhry
      841 rrqkkasaaq ahrvlrlpst wgssrpsiig rgedffvryg dkereavasf dfldeivpps
      901 srgsntslid lddtpsaelr gdimklspl
//
LOCUS       XP_034183130             268 aa            linear   INV 03-SEP-2025
DEFINITION  leukocyte surface antigen CD53 [Osmia lignaria lignaria].
ACCESSION   XP_034183130
VERSION     XP_034183130.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327239.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034183130.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="leukocyte surface antigen CD53"
                     /calculated_mol_wt=29473
     Region          8..>143
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          106..196
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cd03127"
                     /db_xref="CDD:239401"
     Site            order(109,113,115..116,119,131,134..135,138..139)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:239401"
     CDS             1..268
                     /gene="LOC117605662"
                     /coded_by="XM_034327239.2:146..952"
                     /db_xref="GeneID:117605662"
ORIGIN      
        1 martlvclry fliggaivvg vcgliesica syfiyqlyey spltpsnvcg apitllvmgl
       61 itctigwcaw qfldftntgq viifsislii itiantsagi wtlvrheqvd llptadleqv
      121 fkhaisddks lwdhmhsklh ccgingpady hgqdaipwsc cdttsganps dnkgvcatly
      181 aigcqhvvmk rtrsillhvf llalctillq vcfitcmscy vkacrerrer rkeiisasqs
      241 faraskdlgt ndnllgqqmk yskaatdi
//
LOCUS       XP_034183131             236 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 6 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034183131
VERSION     XP_034183131.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327240.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="tetraspanin 6 isoform X1"
                     /calculated_mol_wt=25242
     Region          8..224
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          <132..196
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cl02781"
                     /db_xref="CDD:351888"
     CDS             1..236
                     /gene="Tspan6"
                     /coded_by="XM_034327240.2:171..881"
                     /db_xref="GeneID:117605663"
ORIGIN      
        1 mgcgmglvkt llfvfnfifa vcglgilavg vivhlelasv sqhldtnimf psitlivlgs
       61 iifvisffgc cgairsshcm ivtfasmlmf illiqvavav yafvvikqvd pdtvrkgyne
      121 lfqtyptevq nreivdivqa afkccgvgsk tdysphdnfn gtipwsccgk pegqscsmne
      181 sydkgcaqel esalktagtl lggvavgiag veligiifal clansvrnae rrgyrv
//
LOCUS       XP_034183132             236 aa            linear   INV 03-SEP-2025
DEFINITION  tetraspanin 6 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034183132
VERSION     XP_034183132.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034327241.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..236
                     /product="tetraspanin 6 isoform X2"
                     /calculated_mol_wt=25447
     Region          8..224
                     /region_name="Tetraspanin"
                     /note="Tetraspanin family; pfam00335"
                     /db_xref="CDD:459767"
     Region          <132..196
                     /region_name="tetraspanin_LEL"
                     /note="Tetraspanin, extracellular domain or large
                     extracellular loop (LEL). Tetraspanins are trans-membrane
                     proteins with 4 trans-membrane segments. Both the N- and
                     C-termini lie on the intracellular side of the membrane.
                     This alignment model spans the...; cl02781"
                     /db_xref="CDD:351888"
     CDS             1..236
                     /gene="Tspan6"
                     /coded_by="XM_034327241.2:149..859"
                     /db_xref="GeneID:117605663"
ORIGIN      
        1 mscvsdciqw llyifnfifa vcglgilavg vivhlelasv sqhldtnimf psitlivlgs
       61 iifvisffgc cgairsshcm ivtfasmlmf illiqvavav yafvvikqvd pdtvrkgyne
      121 lfqtyptevq nreivdivqa afkccgvgsk tdysphdnfn gtipwsccgk pegqscsmne
      181 sydkgcaqel esalktagtl lggvavgiag veligiifal clansvrnae rrgyrv
//
LOCUS       XP_034186576            2581 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607239 [Osmia lignaria lignaria].
ACCESSION   XP_034186576
VERSION     XP_034186576.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330685.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186576.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2581
                     /product="uncharacterized protein LOC117607239"
                     /calculated_mol_wt=280504
     Region          <2290..2422
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          2301..2332
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(2302,2306..2307,2310..2312,2314..2315,2319,2322,
                     2332,2334,2336,2340..2341,2344..2346,2348..2349,2353,2356,
                     2365,2367,2369,2373..2374,2377..2379,2381..2382,2386,2389)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          2334..2365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2367..2392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2464..2576
                     /region_name="PUFD_like"
                     /note="PCGF Ub-like fold discriminator and related
                     domains; cl21640"
                     /db_xref="CDD:473927"
     Site            order(2465..2466,2468..2472,2474..2476,2478,2480,2485,
                     2487,2489,2542..2544,2546..2547,2563,2574,2576)
                     /site_type="other"
                     /note="RAWUL domain interface [polypeptide binding]"
                     /db_xref="CDD:271222"
     CDS             1..2581
                     /gene="LOC117607239"
                     /coded_by="XM_034330685.2:1643..9388"
                     /db_xref="GeneID:117607239"
ORIGIN      
        1 mdvsftnvle garqyfqglp vpstggatas adhnlatsts sasststshe hgtasvtvap
       61 pspappapsa ppplslpsqs npatssansn tnsasnnnnt dqqttrylsd vrpssvdnaa
      121 tsssgfwsps vepyppqptr vevpdtrpgd espsmeasss sniitltemq psnsrsssas
      181 tfsqkqasss tslndlhhrs qptttataps sprlhqmqpp pqphspapvy qqlnpvgrts
      241 fstaeilast hqstyqtand rqsqssapvv aqrtqyypry hhpditksap vpfsqnsiyq
      301 ssnvaapssg nvlpptrttp aeqsnigsvp sqgrvqeqrv ptcygnnaip pmntasnygp
      361 sasqnyrasp hqqqqnrsss stnshldrsh ssrlssspsc ssanpcsprh agnashipss
      421 sssssssssf qnlpahipss hmpsagtvhp qhhpqqqqqq qssqmqhqls srinasphsi
      481 gnqsyggrmm vhgvsassss ssssssssip assssnqmpp pppalsgyhp vasphdvphh
      541 atpsphrqsp hvstlhnpha sphhtpsphs sfqphsptyp pppppppppp psvatstphs
      601 ynlpittqhy qpstgyaanp ygmppqssyh sfqknsqpvh tqpaasyypq sgrsqsqpyv
      661 qqmpmpappq sicsgsgnsn agtgyqqskp vtyngvdskr nsmelpygsy rspqtsvprg
      721 gsnthnlppi aalssyhsnr retaskaqam qrqraysaas ttnkvsvvtp pisssmaapq
      781 rvpeypvtla gmnhampvng rtttvtnssy sryanqhgys tyattmapsh hannsssntt
      841 vtsigvnvat rssapattmh ayatdassrt pssyhlqpap iranedygyk eypyqnsstp
      901 tvqstlassi vtpppqtngv rkrespldls vktvktsads taqddvetia tdkhvtsstv
      961 vssrnnsart mlpppaqppp qtatypvydn rpssnntars lpptssnvrt ttpqtvcapk
     1021 vdflpnfnsa plrnhhgqph entlqrrsst qqlyapppqs qhpnntaplp hmstfkktll
     1081 pttqvydgvt apappppapp ssylsandtr ssrypasmld parigpaalp lqdyppsdps
     1141 kyyldsrnkf gqpapvqrdr tgckrpgeti ygtggpnkqp rldmwiqqkl stakalyeqq
     1201 krqemnlpvp lpngtlvapn tyeqrsdsgy sssessryqq aycdkrsyae skvphsspay
     1261 nhpviakpan vhslpqspst ghsaypnyrp sqkaavpppl gaasggqpae ppsntgadkr
     1321 vlsllrnsle nkqqreeqln sqqpilanhg qqsfqnkvva pvepktnigr hnlspftaas
     1381 llernsntpp hykfhvprai dsitqeaprs lygsrvnssa tlpgkeallg prgaenqihr
     1441 dkddglaail aakirtkael kqvgtghfts kpttvpsqds sstpkepdsa aststpqsgs
     1501 sagsppkltr ekvaclpprr rlfsrteeen mpvaatvpvp vpapaiassv parasgfrss
     1561 setsvfdfre sdsegempvl erqtleemrr drkqltkvqp pvplndamcm smtsstdlvk
     1621 ielkendkit evdtfwsstc dkfmeqlrtg damkkrirkk kvsctitskl edtgnesske
     1681 sdpntsqikp edikkeiedv gspvevkdes pkipikeeet pmmkevklev kvepevcked
     1741 tknssdeevp lirrtkrkgk kdesdceeel isrkrrvrrg sriklqsssg sessseesda
     1801 stefgpgstv adrlrarkrs ttnnvdsegm klrsrdttpq kakgekeskg stskttattq
     1861 kgkpkplfgd gsdfrpgwee evyvykkslr mpprlitvsk psrfhrlsts lpdldpgspa
     1921 lsvsmdssdv clgrkkvvds dvesnysfst atfglgskid deeatsstti scppkamkhs
     1981 ktegssivdv laqkvgtskk eqkrkqkekl dknggkllqk gsnepellpt psltpllets
     2041 kgkspvkdkl lkpikvtdsf llgyfrketv nnfrdtfknn halpnefstl vlksrtrtet
     2101 rvlkkqatir evfgedrpas appmqnhddt sqdddsqnet pentlgkvkt lkqkvvsrlk
     2161 nvgilrshka imnskrhlli akrrkdllks laekklqslk tevdeeathe etndaqeven
     2221 nelddetnes evpnkkklkl rtsrrkfrsg fdyirkkkkp lkkdetlpke rkrqvlarps
     2281 pecvadiqse irtwvikkgv getmlhraar lgytdvtayc leklnnapsp kdnagytplh
     2341 eacskghlei aklllaygan asesanggir plheaaenga telvrlllsy gadpllatys
     2401 gqtplmlaae tdaysileqh lddiqghast pwsfggpsti fdpeetgynp lddppmnnpe
     2461 peldeiemev sdvnlpvlfs ltndsdkwvl mqdlmaalri ksrdallrqv npkassgppa
     2521 vahrevmkel klqdfleqsr cchllsgger invrgskvtl ikyndkvrsl lnaekvvisl
     2581 r
//
LOCUS       XP_034186577            2581 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607239 [Osmia lignaria lignaria].
ACCESSION   XP_034186577
VERSION     XP_034186577.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330686.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186577.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2581
                     /product="uncharacterized protein LOC117607239"
                     /calculated_mol_wt=280504
     Region          <2290..2422
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          2301..2332
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(2302,2306..2307,2310..2312,2314..2315,2319,2322,
                     2332,2334,2336,2340..2341,2344..2346,2348..2349,2353,2356,
                     2365,2367,2369,2373..2374,2377..2379,2381..2382,2386,2389)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          2334..2365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2367..2392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2464..2576
                     /region_name="PUFD_like"
                     /note="PCGF Ub-like fold discriminator and related
                     domains; cl21640"
                     /db_xref="CDD:473927"
     Site            order(2465..2466,2468..2472,2474..2476,2478,2480,2485,
                     2487,2489,2542..2544,2546..2547,2563,2574,2576)
                     /site_type="other"
                     /note="RAWUL domain interface [polypeptide binding]"
                     /db_xref="CDD:271222"
     CDS             1..2581
                     /gene="LOC117607239"
                     /coded_by="XM_034330686.2:1064..8809"
                     /db_xref="GeneID:117607239"
ORIGIN      
        1 mdvsftnvle garqyfqglp vpstggatas adhnlatsts sasststshe hgtasvtvap
       61 pspappapsa ppplslpsqs npatssansn tnsasnnnnt dqqttrylsd vrpssvdnaa
      121 tsssgfwsps vepyppqptr vevpdtrpgd espsmeasss sniitltemq psnsrsssas
      181 tfsqkqasss tslndlhhrs qptttataps sprlhqmqpp pqphspapvy qqlnpvgrts
      241 fstaeilast hqstyqtand rqsqssapvv aqrtqyypry hhpditksap vpfsqnsiyq
      301 ssnvaapssg nvlpptrttp aeqsnigsvp sqgrvqeqrv ptcygnnaip pmntasnygp
      361 sasqnyrasp hqqqqnrsss stnshldrsh ssrlssspsc ssanpcsprh agnashipss
      421 sssssssssf qnlpahipss hmpsagtvhp qhhpqqqqqq qssqmqhqls srinasphsi
      481 gnqsyggrmm vhgvsassss ssssssssip assssnqmpp pppalsgyhp vasphdvphh
      541 atpsphrqsp hvstlhnpha sphhtpsphs sfqphsptyp pppppppppp psvatstphs
      601 ynlpittqhy qpstgyaanp ygmppqssyh sfqknsqpvh tqpaasyypq sgrsqsqpyv
      661 qqmpmpappq sicsgsgnsn agtgyqqskp vtyngvdskr nsmelpygsy rspqtsvprg
      721 gsnthnlppi aalssyhsnr retaskaqam qrqraysaas ttnkvsvvtp pisssmaapq
      781 rvpeypvtla gmnhampvng rtttvtnssy sryanqhgys tyattmapsh hannsssntt
      841 vtsigvnvat rssapattmh ayatdassrt pssyhlqpap iranedygyk eypyqnsstp
      901 tvqstlassi vtpppqtngv rkrespldls vktvktsads taqddvetia tdkhvtsstv
      961 vssrnnsart mlpppaqppp qtatypvydn rpssnntars lpptssnvrt ttpqtvcapk
     1021 vdflpnfnsa plrnhhgqph entlqrrsst qqlyapppqs qhpnntaplp hmstfkktll
     1081 pttqvydgvt apappppapp ssylsandtr ssrypasmld parigpaalp lqdyppsdps
     1141 kyyldsrnkf gqpapvqrdr tgckrpgeti ygtggpnkqp rldmwiqqkl stakalyeqq
     1201 krqemnlpvp lpngtlvapn tyeqrsdsgy sssessryqq aycdkrsyae skvphsspay
     1261 nhpviakpan vhslpqspst ghsaypnyrp sqkaavpppl gaasggqpae ppsntgadkr
     1321 vlsllrnsle nkqqreeqln sqqpilanhg qqsfqnkvva pvepktnigr hnlspftaas
     1381 llernsntpp hykfhvprai dsitqeaprs lygsrvnssa tlpgkeallg prgaenqihr
     1441 dkddglaail aakirtkael kqvgtghfts kpttvpsqds sstpkepdsa aststpqsgs
     1501 sagsppkltr ekvaclpprr rlfsrteeen mpvaatvpvp vpapaiassv parasgfrss
     1561 setsvfdfre sdsegempvl erqtleemrr drkqltkvqp pvplndamcm smtsstdlvk
     1621 ielkendkit evdtfwsstc dkfmeqlrtg damkkrirkk kvsctitskl edtgnesske
     1681 sdpntsqikp edikkeiedv gspvevkdes pkipikeeet pmmkevklev kvepevcked
     1741 tknssdeevp lirrtkrkgk kdesdceeel isrkrrvrrg sriklqsssg sessseesda
     1801 stefgpgstv adrlrarkrs ttnnvdsegm klrsrdttpq kakgekeskg stskttattq
     1861 kgkpkplfgd gsdfrpgwee evyvykkslr mpprlitvsk psrfhrlsts lpdldpgspa
     1921 lsvsmdssdv clgrkkvvds dvesnysfst atfglgskid deeatsstti scppkamkhs
     1981 ktegssivdv laqkvgtskk eqkrkqkekl dknggkllqk gsnepellpt psltpllets
     2041 kgkspvkdkl lkpikvtdsf llgyfrketv nnfrdtfknn halpnefstl vlksrtrtet
     2101 rvlkkqatir evfgedrpas appmqnhddt sqdddsqnet pentlgkvkt lkqkvvsrlk
     2161 nvgilrshka imnskrhlli akrrkdllks laekklqslk tevdeeathe etndaqeven
     2221 nelddetnes evpnkkklkl rtsrrkfrsg fdyirkkkkp lkkdetlpke rkrqvlarps
     2281 pecvadiqse irtwvikkgv getmlhraar lgytdvtayc leklnnapsp kdnagytplh
     2341 eacskghlei aklllaygan asesanggir plheaaenga telvrlllsy gadpllatys
     2401 gqtplmlaae tdaysileqh lddiqghast pwsfggpsti fdpeetgynp lddppmnnpe
     2461 peldeiemev sdvnlpvlfs ltndsdkwvl mqdlmaalri ksrdallrqv npkassgppa
     2521 vahrevmkel klqdfleqsr cchllsgger invrgskvtl ikyndkvrsl lnaekvvisl
     2581 r
//
LOCUS       XP_034186578            2581 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607239 [Osmia lignaria lignaria].
ACCESSION   XP_034186578
VERSION     XP_034186578.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330687.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186578.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2581
                     /product="uncharacterized protein LOC117607239"
                     /calculated_mol_wt=280504
     Region          <2290..2422
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          2301..2332
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(2302,2306..2307,2310..2312,2314..2315,2319,2322,
                     2332,2334,2336,2340..2341,2344..2346,2348..2349,2353,2356,
                     2365,2367,2369,2373..2374,2377..2379,2381..2382,2386,2389)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          2334..2365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2367..2392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2464..2576
                     /region_name="PUFD_like"
                     /note="PCGF Ub-like fold discriminator and related
                     domains; cl21640"
                     /db_xref="CDD:473927"
     Site            order(2465..2466,2468..2472,2474..2476,2478,2480,2485,
                     2487,2489,2542..2544,2546..2547,2563,2574,2576)
                     /site_type="other"
                     /note="RAWUL domain interface [polypeptide binding]"
                     /db_xref="CDD:271222"
     CDS             1..2581
                     /gene="LOC117607239"
                     /coded_by="XM_034330687.2:1700..9445"
                     /db_xref="GeneID:117607239"
ORIGIN      
        1 mdvsftnvle garqyfqglp vpstggatas adhnlatsts sasststshe hgtasvtvap
       61 pspappapsa ppplslpsqs npatssansn tnsasnnnnt dqqttrylsd vrpssvdnaa
      121 tsssgfwsps vepyppqptr vevpdtrpgd espsmeasss sniitltemq psnsrsssas
      181 tfsqkqasss tslndlhhrs qptttataps sprlhqmqpp pqphspapvy qqlnpvgrts
      241 fstaeilast hqstyqtand rqsqssapvv aqrtqyypry hhpditksap vpfsqnsiyq
      301 ssnvaapssg nvlpptrttp aeqsnigsvp sqgrvqeqrv ptcygnnaip pmntasnygp
      361 sasqnyrasp hqqqqnrsss stnshldrsh ssrlssspsc ssanpcsprh agnashipss
      421 sssssssssf qnlpahipss hmpsagtvhp qhhpqqqqqq qssqmqhqls srinasphsi
      481 gnqsyggrmm vhgvsassss ssssssssip assssnqmpp pppalsgyhp vasphdvphh
      541 atpsphrqsp hvstlhnpha sphhtpsphs sfqphsptyp pppppppppp psvatstphs
      601 ynlpittqhy qpstgyaanp ygmppqssyh sfqknsqpvh tqpaasyypq sgrsqsqpyv
      661 qqmpmpappq sicsgsgnsn agtgyqqskp vtyngvdskr nsmelpygsy rspqtsvprg
      721 gsnthnlppi aalssyhsnr retaskaqam qrqraysaas ttnkvsvvtp pisssmaapq
      781 rvpeypvtla gmnhampvng rtttvtnssy sryanqhgys tyattmapsh hannsssntt
      841 vtsigvnvat rssapattmh ayatdassrt pssyhlqpap iranedygyk eypyqnsstp
      901 tvqstlassi vtpppqtngv rkrespldls vktvktsads taqddvetia tdkhvtsstv
      961 vssrnnsart mlpppaqppp qtatypvydn rpssnntars lpptssnvrt ttpqtvcapk
     1021 vdflpnfnsa plrnhhgqph entlqrrsst qqlyapppqs qhpnntaplp hmstfkktll
     1081 pttqvydgvt apappppapp ssylsandtr ssrypasmld parigpaalp lqdyppsdps
     1141 kyyldsrnkf gqpapvqrdr tgckrpgeti ygtggpnkqp rldmwiqqkl stakalyeqq
     1201 krqemnlpvp lpngtlvapn tyeqrsdsgy sssessryqq aycdkrsyae skvphsspay
     1261 nhpviakpan vhslpqspst ghsaypnyrp sqkaavpppl gaasggqpae ppsntgadkr
     1321 vlsllrnsle nkqqreeqln sqqpilanhg qqsfqnkvva pvepktnigr hnlspftaas
     1381 llernsntpp hykfhvprai dsitqeaprs lygsrvnssa tlpgkeallg prgaenqihr
     1441 dkddglaail aakirtkael kqvgtghfts kpttvpsqds sstpkepdsa aststpqsgs
     1501 sagsppkltr ekvaclpprr rlfsrteeen mpvaatvpvp vpapaiassv parasgfrss
     1561 setsvfdfre sdsegempvl erqtleemrr drkqltkvqp pvplndamcm smtsstdlvk
     1621 ielkendkit evdtfwsstc dkfmeqlrtg damkkrirkk kvsctitskl edtgnesske
     1681 sdpntsqikp edikkeiedv gspvevkdes pkipikeeet pmmkevklev kvepevcked
     1741 tknssdeevp lirrtkrkgk kdesdceeel isrkrrvrrg sriklqsssg sessseesda
     1801 stefgpgstv adrlrarkrs ttnnvdsegm klrsrdttpq kakgekeskg stskttattq
     1861 kgkpkplfgd gsdfrpgwee evyvykkslr mpprlitvsk psrfhrlsts lpdldpgspa
     1921 lsvsmdssdv clgrkkvvds dvesnysfst atfglgskid deeatsstti scppkamkhs
     1981 ktegssivdv laqkvgtskk eqkrkqkekl dknggkllqk gsnepellpt psltpllets
     2041 kgkspvkdkl lkpikvtdsf llgyfrketv nnfrdtfknn halpnefstl vlksrtrtet
     2101 rvlkkqatir evfgedrpas appmqnhddt sqdddsqnet pentlgkvkt lkqkvvsrlk
     2161 nvgilrshka imnskrhlli akrrkdllks laekklqslk tevdeeathe etndaqeven
     2221 nelddetnes evpnkkklkl rtsrrkfrsg fdyirkkkkp lkkdetlpke rkrqvlarps
     2281 pecvadiqse irtwvikkgv getmlhraar lgytdvtayc leklnnapsp kdnagytplh
     2341 eacskghlei aklllaygan asesanggir plheaaenga telvrlllsy gadpllatys
     2401 gqtplmlaae tdaysileqh lddiqghast pwsfggpsti fdpeetgynp lddppmnnpe
     2461 peldeiemev sdvnlpvlfs ltndsdkwvl mqdlmaalri ksrdallrqv npkassgppa
     2521 vahrevmkel klqdfleqsr cchllsgger invrgskvtl ikyndkvrsl lnaekvvisl
     2581 r
//
LOCUS       XP_034186579            2581 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607239 [Osmia lignaria lignaria].
ACCESSION   XP_034186579
VERSION     XP_034186579.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330688.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186579.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2581
                     /product="uncharacterized protein LOC117607239"
                     /calculated_mol_wt=280504
     Region          <2290..2422
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          2301..2332
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(2302,2306..2307,2310..2312,2314..2315,2319,2322,
                     2332,2334,2336,2340..2341,2344..2346,2348..2349,2353,2356,
                     2365,2367,2369,2373..2374,2377..2379,2381..2382,2386,2389)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          2334..2365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2367..2392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2464..2576
                     /region_name="PUFD_like"
                     /note="PCGF Ub-like fold discriminator and related
                     domains; cl21640"
                     /db_xref="CDD:473927"
     Site            order(2465..2466,2468..2472,2474..2476,2478,2480,2485,
                     2487,2489,2542..2544,2546..2547,2563,2574,2576)
                     /site_type="other"
                     /note="RAWUL domain interface [polypeptide binding]"
                     /db_xref="CDD:271222"
     CDS             1..2581
                     /gene="LOC117607239"
                     /coded_by="XM_034330688.2:701..8446"
                     /db_xref="GeneID:117607239"
ORIGIN      
        1 mdvsftnvle garqyfqglp vpstggatas adhnlatsts sasststshe hgtasvtvap
       61 pspappapsa ppplslpsqs npatssansn tnsasnnnnt dqqttrylsd vrpssvdnaa
      121 tsssgfwsps vepyppqptr vevpdtrpgd espsmeasss sniitltemq psnsrsssas
      181 tfsqkqasss tslndlhhrs qptttataps sprlhqmqpp pqphspapvy qqlnpvgrts
      241 fstaeilast hqstyqtand rqsqssapvv aqrtqyypry hhpditksap vpfsqnsiyq
      301 ssnvaapssg nvlpptrttp aeqsnigsvp sqgrvqeqrv ptcygnnaip pmntasnygp
      361 sasqnyrasp hqqqqnrsss stnshldrsh ssrlssspsc ssanpcsprh agnashipss
      421 sssssssssf qnlpahipss hmpsagtvhp qhhpqqqqqq qssqmqhqls srinasphsi
      481 gnqsyggrmm vhgvsassss ssssssssip assssnqmpp pppalsgyhp vasphdvphh
      541 atpsphrqsp hvstlhnpha sphhtpsphs sfqphsptyp pppppppppp psvatstphs
      601 ynlpittqhy qpstgyaanp ygmppqssyh sfqknsqpvh tqpaasyypq sgrsqsqpyv
      661 qqmpmpappq sicsgsgnsn agtgyqqskp vtyngvdskr nsmelpygsy rspqtsvprg
      721 gsnthnlppi aalssyhsnr retaskaqam qrqraysaas ttnkvsvvtp pisssmaapq
      781 rvpeypvtla gmnhampvng rtttvtnssy sryanqhgys tyattmapsh hannsssntt
      841 vtsigvnvat rssapattmh ayatdassrt pssyhlqpap iranedygyk eypyqnsstp
      901 tvqstlassi vtpppqtngv rkrespldls vktvktsads taqddvetia tdkhvtsstv
      961 vssrnnsart mlpppaqppp qtatypvydn rpssnntars lpptssnvrt ttpqtvcapk
     1021 vdflpnfnsa plrnhhgqph entlqrrsst qqlyapppqs qhpnntaplp hmstfkktll
     1081 pttqvydgvt apappppapp ssylsandtr ssrypasmld parigpaalp lqdyppsdps
     1141 kyyldsrnkf gqpapvqrdr tgckrpgeti ygtggpnkqp rldmwiqqkl stakalyeqq
     1201 krqemnlpvp lpngtlvapn tyeqrsdsgy sssessryqq aycdkrsyae skvphsspay
     1261 nhpviakpan vhslpqspst ghsaypnyrp sqkaavpppl gaasggqpae ppsntgadkr
     1321 vlsllrnsle nkqqreeqln sqqpilanhg qqsfqnkvva pvepktnigr hnlspftaas
     1381 llernsntpp hykfhvprai dsitqeaprs lygsrvnssa tlpgkeallg prgaenqihr
     1441 dkddglaail aakirtkael kqvgtghfts kpttvpsqds sstpkepdsa aststpqsgs
     1501 sagsppkltr ekvaclpprr rlfsrteeen mpvaatvpvp vpapaiassv parasgfrss
     1561 setsvfdfre sdsegempvl erqtleemrr drkqltkvqp pvplndamcm smtsstdlvk
     1621 ielkendkit evdtfwsstc dkfmeqlrtg damkkrirkk kvsctitskl edtgnesske
     1681 sdpntsqikp edikkeiedv gspvevkdes pkipikeeet pmmkevklev kvepevcked
     1741 tknssdeevp lirrtkrkgk kdesdceeel isrkrrvrrg sriklqsssg sessseesda
     1801 stefgpgstv adrlrarkrs ttnnvdsegm klrsrdttpq kakgekeskg stskttattq
     1861 kgkpkplfgd gsdfrpgwee evyvykkslr mpprlitvsk psrfhrlsts lpdldpgspa
     1921 lsvsmdssdv clgrkkvvds dvesnysfst atfglgskid deeatsstti scppkamkhs
     1981 ktegssivdv laqkvgtskk eqkrkqkekl dknggkllqk gsnepellpt psltpllets
     2041 kgkspvkdkl lkpikvtdsf llgyfrketv nnfrdtfknn halpnefstl vlksrtrtet
     2101 rvlkkqatir evfgedrpas appmqnhddt sqdddsqnet pentlgkvkt lkqkvvsrlk
     2161 nvgilrshka imnskrhlli akrrkdllks laekklqslk tevdeeathe etndaqeven
     2221 nelddetnes evpnkkklkl rtsrrkfrsg fdyirkkkkp lkkdetlpke rkrqvlarps
     2281 pecvadiqse irtwvikkgv getmlhraar lgytdvtayc leklnnapsp kdnagytplh
     2341 eacskghlei aklllaygan asesanggir plheaaenga telvrlllsy gadpllatys
     2401 gqtplmlaae tdaysileqh lddiqghast pwsfggpsti fdpeetgynp lddppmnnpe
     2461 peldeiemev sdvnlpvlfs ltndsdkwvl mqdlmaalri ksrdallrqv npkassgppa
     2521 vahrevmkel klqdfleqsr cchllsgger invrgskvtl ikyndkvrsl lnaekvvisl
     2581 r
//
LOCUS       XP_034186580            2581 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607239 [Osmia lignaria lignaria].
ACCESSION   XP_034186580
VERSION     XP_034186580.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330689.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186580.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2581
                     /product="uncharacterized protein LOC117607239"
                     /calculated_mol_wt=280504
     Region          <2290..2422
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          2301..2332
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(2302,2306..2307,2310..2312,2314..2315,2319,2322,
                     2332,2334,2336,2340..2341,2344..2346,2348..2349,2353,2356,
                     2365,2367,2369,2373..2374,2377..2379,2381..2382,2386,2389)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          2334..2365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2367..2392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2464..2576
                     /region_name="PUFD_like"
                     /note="PCGF Ub-like fold discriminator and related
                     domains; cl21640"
                     /db_xref="CDD:473927"
     Site            order(2465..2466,2468..2472,2474..2476,2478,2480,2485,
                     2487,2489,2542..2544,2546..2547,2563,2574,2576)
                     /site_type="other"
                     /note="RAWUL domain interface [polypeptide binding]"
                     /db_xref="CDD:271222"
     CDS             1..2581
                     /gene="LOC117607239"
                     /coded_by="XM_034330689.2:1529..9274"
                     /db_xref="GeneID:117607239"
ORIGIN      
        1 mdvsftnvle garqyfqglp vpstggatas adhnlatsts sasststshe hgtasvtvap
       61 pspappapsa ppplslpsqs npatssansn tnsasnnnnt dqqttrylsd vrpssvdnaa
      121 tsssgfwsps vepyppqptr vevpdtrpgd espsmeasss sniitltemq psnsrsssas
      181 tfsqkqasss tslndlhhrs qptttataps sprlhqmqpp pqphspapvy qqlnpvgrts
      241 fstaeilast hqstyqtand rqsqssapvv aqrtqyypry hhpditksap vpfsqnsiyq
      301 ssnvaapssg nvlpptrttp aeqsnigsvp sqgrvqeqrv ptcygnnaip pmntasnygp
      361 sasqnyrasp hqqqqnrsss stnshldrsh ssrlssspsc ssanpcsprh agnashipss
      421 sssssssssf qnlpahipss hmpsagtvhp qhhpqqqqqq qssqmqhqls srinasphsi
      481 gnqsyggrmm vhgvsassss ssssssssip assssnqmpp pppalsgyhp vasphdvphh
      541 atpsphrqsp hvstlhnpha sphhtpsphs sfqphsptyp pppppppppp psvatstphs
      601 ynlpittqhy qpstgyaanp ygmppqssyh sfqknsqpvh tqpaasyypq sgrsqsqpyv
      661 qqmpmpappq sicsgsgnsn agtgyqqskp vtyngvdskr nsmelpygsy rspqtsvprg
      721 gsnthnlppi aalssyhsnr retaskaqam qrqraysaas ttnkvsvvtp pisssmaapq
      781 rvpeypvtla gmnhampvng rtttvtnssy sryanqhgys tyattmapsh hannsssntt
      841 vtsigvnvat rssapattmh ayatdassrt pssyhlqpap iranedygyk eypyqnsstp
      901 tvqstlassi vtpppqtngv rkrespldls vktvktsads taqddvetia tdkhvtsstv
      961 vssrnnsart mlpppaqppp qtatypvydn rpssnntars lpptssnvrt ttpqtvcapk
     1021 vdflpnfnsa plrnhhgqph entlqrrsst qqlyapppqs qhpnntaplp hmstfkktll
     1081 pttqvydgvt apappppapp ssylsandtr ssrypasmld parigpaalp lqdyppsdps
     1141 kyyldsrnkf gqpapvqrdr tgckrpgeti ygtggpnkqp rldmwiqqkl stakalyeqq
     1201 krqemnlpvp lpngtlvapn tyeqrsdsgy sssessryqq aycdkrsyae skvphsspay
     1261 nhpviakpan vhslpqspst ghsaypnyrp sqkaavpppl gaasggqpae ppsntgadkr
     1321 vlsllrnsle nkqqreeqln sqqpilanhg qqsfqnkvva pvepktnigr hnlspftaas
     1381 llernsntpp hykfhvprai dsitqeaprs lygsrvnssa tlpgkeallg prgaenqihr
     1441 dkddglaail aakirtkael kqvgtghfts kpttvpsqds sstpkepdsa aststpqsgs
     1501 sagsppkltr ekvaclpprr rlfsrteeen mpvaatvpvp vpapaiassv parasgfrss
     1561 setsvfdfre sdsegempvl erqtleemrr drkqltkvqp pvplndamcm smtsstdlvk
     1621 ielkendkit evdtfwsstc dkfmeqlrtg damkkrirkk kvsctitskl edtgnesske
     1681 sdpntsqikp edikkeiedv gspvevkdes pkipikeeet pmmkevklev kvepevcked
     1741 tknssdeevp lirrtkrkgk kdesdceeel isrkrrvrrg sriklqsssg sessseesda
     1801 stefgpgstv adrlrarkrs ttnnvdsegm klrsrdttpq kakgekeskg stskttattq
     1861 kgkpkplfgd gsdfrpgwee evyvykkslr mpprlitvsk psrfhrlsts lpdldpgspa
     1921 lsvsmdssdv clgrkkvvds dvesnysfst atfglgskid deeatsstti scppkamkhs
     1981 ktegssivdv laqkvgtskk eqkrkqkekl dknggkllqk gsnepellpt psltpllets
     2041 kgkspvkdkl lkpikvtdsf llgyfrketv nnfrdtfknn halpnefstl vlksrtrtet
     2101 rvlkkqatir evfgedrpas appmqnhddt sqdddsqnet pentlgkvkt lkqkvvsrlk
     2161 nvgilrshka imnskrhlli akrrkdllks laekklqslk tevdeeathe etndaqeven
     2221 nelddetnes evpnkkklkl rtsrrkfrsg fdyirkkkkp lkkdetlpke rkrqvlarps
     2281 pecvadiqse irtwvikkgv getmlhraar lgytdvtayc leklnnapsp kdnagytplh
     2341 eacskghlei aklllaygan asesanggir plheaaenga telvrlllsy gadpllatys
     2401 gqtplmlaae tdaysileqh lddiqghast pwsfggpsti fdpeetgynp lddppmnnpe
     2461 peldeiemev sdvnlpvlfs ltndsdkwvl mqdlmaalri ksrdallrqv npkassgppa
     2521 vahrevmkel klqdfleqsr cchllsgger invrgskvtl ikyndkvrsl lnaekvvisl
     2581 r
//
LOCUS       XP_034186581            2581 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607239 [Osmia lignaria lignaria].
ACCESSION   XP_034186581
VERSION     XP_034186581.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330690.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186581.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2581
                     /product="uncharacterized protein LOC117607239"
                     /calculated_mol_wt=280504
     Region          <2290..2422
                     /region_name="ANKYR"
                     /note="Ankyrin repeat [Signal transduction mechanisms];
                     COG0666"
                     /db_xref="CDD:440430"
     Region          2301..2332
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Site            order(2302,2306..2307,2310..2312,2314..2315,2319,2322,
                     2332,2334,2336,2340..2341,2344..2346,2348..2349,2353,2356,
                     2365,2367,2369,2373..2374,2377..2379,2381..2382,2386,2389)
                     /site_type="other"
                     /note="oligomer interface [polypeptide binding]"
                     /db_xref="CDD:293786"
     Region          2334..2365
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2367..2392
                     /region_name="ANK repeat"
                     /note="ANK repeat [structural motif]"
                     /db_xref="CDD:293786"
     Region          2464..2576
                     /region_name="PUFD_like"
                     /note="PCGF Ub-like fold discriminator and related
                     domains; cl21640"
                     /db_xref="CDD:473927"
     Site            order(2465..2466,2468..2472,2474..2476,2478,2480,2485,
                     2487,2489,2542..2544,2546..2547,2563,2574,2576)
                     /site_type="other"
                     /note="RAWUL domain interface [polypeptide binding]"
                     /db_xref="CDD:271222"
     CDS             1..2581
                     /gene="LOC117607239"
                     /coded_by="XM_034330690.2:1573..9318"
                     /db_xref="GeneID:117607239"
ORIGIN      
        1 mdvsftnvle garqyfqglp vpstggatas adhnlatsts sasststshe hgtasvtvap
       61 pspappapsa ppplslpsqs npatssansn tnsasnnnnt dqqttrylsd vrpssvdnaa
      121 tsssgfwsps vepyppqptr vevpdtrpgd espsmeasss sniitltemq psnsrsssas
      181 tfsqkqasss tslndlhhrs qptttataps sprlhqmqpp pqphspapvy qqlnpvgrts
      241 fstaeilast hqstyqtand rqsqssapvv aqrtqyypry hhpditksap vpfsqnsiyq
      301 ssnvaapssg nvlpptrttp aeqsnigsvp sqgrvqeqrv ptcygnnaip pmntasnygp
      361 sasqnyrasp hqqqqnrsss stnshldrsh ssrlssspsc ssanpcsprh agnashipss
      421 sssssssssf qnlpahipss hmpsagtvhp qhhpqqqqqq qssqmqhqls srinasphsi
      481 gnqsyggrmm vhgvsassss ssssssssip assssnqmpp pppalsgyhp vasphdvphh
      541 atpsphrqsp hvstlhnpha sphhtpsphs sfqphsptyp pppppppppp psvatstphs
      601 ynlpittqhy qpstgyaanp ygmppqssyh sfqknsqpvh tqpaasyypq sgrsqsqpyv
      661 qqmpmpappq sicsgsgnsn agtgyqqskp vtyngvdskr nsmelpygsy rspqtsvprg
      721 gsnthnlppi aalssyhsnr retaskaqam qrqraysaas ttnkvsvvtp pisssmaapq
      781 rvpeypvtla gmnhampvng rtttvtnssy sryanqhgys tyattmapsh hannsssntt
      841 vtsigvnvat rssapattmh ayatdassrt pssyhlqpap iranedygyk eypyqnsstp
      901 tvqstlassi vtpppqtngv rkrespldls vktvktsads taqddvetia tdkhvtsstv
      961 vssrnnsart mlpppaqppp qtatypvydn rpssnntars lpptssnvrt ttpqtvcapk
     1021 vdflpnfnsa plrnhhgqph entlqrrsst qqlyapppqs qhpnntaplp hmstfkktll
     1081 pttqvydgvt apappppapp ssylsandtr ssrypasmld parigpaalp lqdyppsdps
     1141 kyyldsrnkf gqpapvqrdr tgckrpgeti ygtggpnkqp rldmwiqqkl stakalyeqq
     1201 krqemnlpvp lpngtlvapn tyeqrsdsgy sssessryqq aycdkrsyae skvphsspay
     1261 nhpviakpan vhslpqspst ghsaypnyrp sqkaavpppl gaasggqpae ppsntgadkr
     1321 vlsllrnsle nkqqreeqln sqqpilanhg qqsfqnkvva pvepktnigr hnlspftaas
     1381 llernsntpp hykfhvprai dsitqeaprs lygsrvnssa tlpgkeallg prgaenqihr
     1441 dkddglaail aakirtkael kqvgtghfts kpttvpsqds sstpkepdsa aststpqsgs
     1501 sagsppkltr ekvaclpprr rlfsrteeen mpvaatvpvp vpapaiassv parasgfrss
     1561 setsvfdfre sdsegempvl erqtleemrr drkqltkvqp pvplndamcm smtsstdlvk
     1621 ielkendkit evdtfwsstc dkfmeqlrtg damkkrirkk kvsctitskl edtgnesske
     1681 sdpntsqikp edikkeiedv gspvevkdes pkipikeeet pmmkevklev kvepevcked
     1741 tknssdeevp lirrtkrkgk kdesdceeel isrkrrvrrg sriklqsssg sessseesda
     1801 stefgpgstv adrlrarkrs ttnnvdsegm klrsrdttpq kakgekeskg stskttattq
     1861 kgkpkplfgd gsdfrpgwee evyvykkslr mpprlitvsk psrfhrlsts lpdldpgspa
     1921 lsvsmdssdv clgrkkvvds dvesnysfst atfglgskid deeatsstti scppkamkhs
     1981 ktegssivdv laqkvgtskk eqkrkqkekl dknggkllqk gsnepellpt psltpllets
     2041 kgkspvkdkl lkpikvtdsf llgyfrketv nnfrdtfknn halpnefstl vlksrtrtet
     2101 rvlkkqatir evfgedrpas appmqnhddt sqdddsqnet pentlgkvkt lkqkvvsrlk
     2161 nvgilrshka imnskrhlli akrrkdllks laekklqslk tevdeeathe etndaqeven
     2221 nelddetnes evpnkkklkl rtsrrkfrsg fdyirkkkkp lkkdetlpke rkrqvlarps
     2281 pecvadiqse irtwvikkgv getmlhraar lgytdvtayc leklnnapsp kdnagytplh
     2341 eacskghlei aklllaygan asesanggir plheaaenga telvrlllsy gadpllatys
     2401 gqtplmlaae tdaysileqh lddiqghast pwsfggpsti fdpeetgynp lddppmnnpe
     2461 peldeiemev sdvnlpvlfs ltndsdkwvl mqdlmaalri ksrdallrqv npkassgppa
     2521 vahrevmkel klqdfleqsr cchllsgger invrgskvtl ikyndkvrsl lnaekvvisl
     2581 r
//
LOCUS       XP_076548230            2176 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548230
VERSION     XP_076548230.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692115.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2176
                     /product="rho guanine nucleotide exchange factor 3 isoform
                     X1"
                     /calculated_mol_wt=241758
     Region          <66..292
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1682..1734
                     /region_name="SH3_ARHGEF9_like"
                     /note="Src homology 3 domain of ARHGEF9-like Rho guanine
                     nucleotide exchange factors; cd11828"
                     /db_xref="CDD:212762"
     Site            order(1684..1686,1691..1692,1694,1730,1733)
                     /site_type="other"
                     /note="DH-PH interface [polypeptide binding]"
                     /db_xref="CDD:212762"
     Site            order(1687,1689,1692,1696,1714..1715,1728,1730..1731)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212762"
     Region          1777..1956
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; smart00325"
                     /db_xref="CDD:214619"
     Site            order(1780,1784,1882,1906..1907,1910..1911,1913..1914,
                     1917..1918,1921..1922,1925,1951,1955)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1961..2099
                     /region_name="PH_Collybistin_ASEF"
                     /note="Collybistin/APC-stimulated guanine nucleotide
                     exchange factor pleckstrin homology (PH) domain; cd01224"
                     /db_xref="CDD:269931"
     CDS             1..2176
                     /gene="RhoGEF3"
                     /coded_by="XM_076692115.1:809..7339"
                     /db_xref="GeneID:117607240"
ORIGIN      
        1 mnrredpllr qhrsrllqla eatnikpgrg sgkrrgqrqs hgfgpsnggp sgggrvtlqd
       61 ivrsdpgytp niehlvfper kssnpnlakt sdespsnksk snptssgrsr laevfsrhys
      121 rgssqdssvt srknhlnsts ldsgatvshq atgsstgatv vtrsggrrwl sqssqstrqh
      181 saedgvrggn vgvigpvsts sssqapapal pprrvspads tdisntatgs rgdrgpnvsi
      241 lhlrgtadpw evgsqgstys igsnksqtgg kgkssgntrg sytrgtsips lkrhdttasa
      301 sstsslkqhr qdshgqvtss rrressnsfl ssnsgtsgel fisgdcsrel spvrwcdrev
      361 dgvylgrsgw vqvqqrslde nrrinyessl vtattgtlpl prratvklad yhcnsepgkc
      421 peeflrldsq rpdylalhgq dfdsvasstc tsphsipesy sppsitpiis pppafqdvig
      481 kkssskhssg rnnygkapfl prsnaivdsd iispppsppr qvnwsslpss srksagtpsr
      541 prrqnttsqq qqqqqqqqqy rmaqaksled qsstrrsqfv qrylesssss sssvgfrsld
      601 scvtrstmpr laentdssve gyddgddedd npssslnlsl vsssiialns snesirange
      661 rispnrqgrh hrsqqglrrs pgssesgkls fnspssssss sssaerqgrs ptpnpfrran
      721 atrqhqsart aqvspeshqs rvrrsrslql pekrspgaaa psrehsresn ephrvvvkis
      781 tdrgnerkrh ilqnrkaqst edalneellr eteavteyly gtrsraitrn lmpsrferre
      841 dsqeqrqrnn lttydvyfis skqqqpqssk ssgssmqqsq qqqqaqqqqq qqqqqqsrrp
      901 ktlqrgattp nanpsssnhd fcispeaklr cnsstcdfwp hcsqretlys psqapvfmkl
      961 sqsypahqrl ptdtgsshvn rgsassspas lermddrefr erensrknrv nreqnnpsts
     1021 kyqdrqpksv lkqpnrwspl egsngngsnv ekrehhrfyq kpeyagsfea cenkdrkvsp
     1081 vqgknivnrs psghvtssst ssssssdiwv ttsdrtvtks prnakssgas tpmedavigs
     1141 lktlieppkd gmlsrpgsap trgedslsad asldphqrsl slpksflthn tdsdkpatrk
     1201 fvgiedrssc lsisnnkgsw qrgqssqrss pspsrlhhtq eaatphtapv splhdqrsna
     1261 ysgkerrrip glskaqrrik asstpalldr dvesrqwsgd eedegttghv ttehplteat
     1321 iplvshsrlq eqpsnlsgni vtasgiqnpa rsstqsqqes vlqkfrksfs lrfhkkgske
     1381 snesecpved ndgsgpldee eerepsnvte qtsqhkedtn sdqkfrfgpl vwrsskerkk
     1441 gskaarnakc nsgdsgiqie mvsggaltgg ggggmigggd sseshdtdaq detdsppalr
     1501 rrvadksrpq selvnqilid kfkadlqsrt srhqhvrrtn sdlggqrllq wdtrsgyshv
     1561 hnyrrmlstp spiktrpprl sprhsshdiv tlrsnakgrs srtnlrrsls qplginqlsp
     1621 lmrtktagar lpggnvlsed eqdgrggtsd demmsdsess iasltdrkks feqtmdeevv
     1681 ilaeavwdhv amepeelafr agdvievldn ldkdwwwgsc rgehgwfpaa fvrlrvsqed
     1741 tvedclaama sggtsnsqlr rrtsvsllsn eqvrtsvvre lvqterdfvk ilrdvaegyi
     1801 aecrrrtdmf tedqietifi nleelldfqs eflkdlesri dwnspykscv gecflnhrag
     1861 frmyseycns hpmatatlqe lyqhnryskf feacrlmrgl ieipldgyll tpvqrickyp
     1921 lqlaellkyt kadhpdyhki qealeamrgv avlinerkrr mesleklaaw qlrvegwege
     1981 dlievssqli yqgdairvtt gmwtnnitlf lfdhqlvyck kdilkrntyv ykgriyldts
     2041 evidvpdgkd hqlgvtvrhc lkvyscvrdk wllfccrtae ekrrwleama eerrlvvqdr
     2101 ndglefpaaa kqlarlaatr lqnrppikpr nktykresay eiptmigqgt tnslgrkvgt
     2161 wftfgsskks trlqps
//
LOCUS       XP_076548231            2176 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548231
VERSION     XP_076548231.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692116.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2176
                     /product="rho guanine nucleotide exchange factor 3 isoform
                     X1"
                     /calculated_mol_wt=241758
     Region          <66..292
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1682..1734
                     /region_name="SH3_ARHGEF9_like"
                     /note="Src homology 3 domain of ARHGEF9-like Rho guanine
                     nucleotide exchange factors; cd11828"
                     /db_xref="CDD:212762"
     Site            order(1684..1686,1691..1692,1694,1730,1733)
                     /site_type="other"
                     /note="DH-PH interface [polypeptide binding]"
                     /db_xref="CDD:212762"
     Site            order(1687,1689,1692,1696,1714..1715,1728,1730..1731)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212762"
     Region          1777..1956
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; smart00325"
                     /db_xref="CDD:214619"
     Site            order(1780,1784,1882,1906..1907,1910..1911,1913..1914,
                     1917..1918,1921..1922,1925,1951,1955)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1961..2099
                     /region_name="PH_Collybistin_ASEF"
                     /note="Collybistin/APC-stimulated guanine nucleotide
                     exchange factor pleckstrin homology (PH) domain; cd01224"
                     /db_xref="CDD:269931"
     CDS             1..2176
                     /gene="RhoGEF3"
                     /coded_by="XM_076692116.1:894..7424"
                     /db_xref="GeneID:117607240"
ORIGIN      
        1 mnrredpllr qhrsrllqla eatnikpgrg sgkrrgqrqs hgfgpsnggp sgggrvtlqd
       61 ivrsdpgytp niehlvfper kssnpnlakt sdespsnksk snptssgrsr laevfsrhys
      121 rgssqdssvt srknhlnsts ldsgatvshq atgsstgatv vtrsggrrwl sqssqstrqh
      181 saedgvrggn vgvigpvsts sssqapapal pprrvspads tdisntatgs rgdrgpnvsi
      241 lhlrgtadpw evgsqgstys igsnksqtgg kgkssgntrg sytrgtsips lkrhdttasa
      301 sstsslkqhr qdshgqvtss rrressnsfl ssnsgtsgel fisgdcsrel spvrwcdrev
      361 dgvylgrsgw vqvqqrslde nrrinyessl vtattgtlpl prratvklad yhcnsepgkc
      421 peeflrldsq rpdylalhgq dfdsvasstc tsphsipesy sppsitpiis pppafqdvig
      481 kkssskhssg rnnygkapfl prsnaivdsd iispppsppr qvnwsslpss srksagtpsr
      541 prrqnttsqq qqqqqqqqqy rmaqaksled qsstrrsqfv qrylesssss sssvgfrsld
      601 scvtrstmpr laentdssve gyddgddedd npssslnlsl vsssiialns snesirange
      661 rispnrqgrh hrsqqglrrs pgssesgkls fnspssssss sssaerqgrs ptpnpfrran
      721 atrqhqsart aqvspeshqs rvrrsrslql pekrspgaaa psrehsresn ephrvvvkis
      781 tdrgnerkrh ilqnrkaqst edalneellr eteavteyly gtrsraitrn lmpsrferre
      841 dsqeqrqrnn lttydvyfis skqqqpqssk ssgssmqqsq qqqqaqqqqq qqqqqqsrrp
      901 ktlqrgattp nanpsssnhd fcispeaklr cnsstcdfwp hcsqretlys psqapvfmkl
      961 sqsypahqrl ptdtgsshvn rgsassspas lermddrefr erensrknrv nreqnnpsts
     1021 kyqdrqpksv lkqpnrwspl egsngngsnv ekrehhrfyq kpeyagsfea cenkdrkvsp
     1081 vqgknivnrs psghvtssst ssssssdiwv ttsdrtvtks prnakssgas tpmedavigs
     1141 lktlieppkd gmlsrpgsap trgedslsad asldphqrsl slpksflthn tdsdkpatrk
     1201 fvgiedrssc lsisnnkgsw qrgqssqrss pspsrlhhtq eaatphtapv splhdqrsna
     1261 ysgkerrrip glskaqrrik asstpalldr dvesrqwsgd eedegttghv ttehplteat
     1321 iplvshsrlq eqpsnlsgni vtasgiqnpa rsstqsqqes vlqkfrksfs lrfhkkgske
     1381 snesecpved ndgsgpldee eerepsnvte qtsqhkedtn sdqkfrfgpl vwrsskerkk
     1441 gskaarnakc nsgdsgiqie mvsggaltgg ggggmigggd sseshdtdaq detdsppalr
     1501 rrvadksrpq selvnqilid kfkadlqsrt srhqhvrrtn sdlggqrllq wdtrsgyshv
     1561 hnyrrmlstp spiktrpprl sprhsshdiv tlrsnakgrs srtnlrrsls qplginqlsp
     1621 lmrtktagar lpggnvlsed eqdgrggtsd demmsdsess iasltdrkks feqtmdeevv
     1681 ilaeavwdhv amepeelafr agdvievldn ldkdwwwgsc rgehgwfpaa fvrlrvsqed
     1741 tvedclaama sggtsnsqlr rrtsvsllsn eqvrtsvvre lvqterdfvk ilrdvaegyi
     1801 aecrrrtdmf tedqietifi nleelldfqs eflkdlesri dwnspykscv gecflnhrag
     1861 frmyseycns hpmatatlqe lyqhnryskf feacrlmrgl ieipldgyll tpvqrickyp
     1921 lqlaellkyt kadhpdyhki qealeamrgv avlinerkrr mesleklaaw qlrvegwege
     1981 dlievssqli yqgdairvtt gmwtnnitlf lfdhqlvyck kdilkrntyv ykgriyldts
     2041 evidvpdgkd hqlgvtvrhc lkvyscvrdk wllfccrtae ekrrwleama eerrlvvqdr
     2101 ndglefpaaa kqlarlaatr lqnrppikpr nktykresay eiptmigqgt tnslgrkvgt
     2161 wftfgsskks trlqps
//
LOCUS       XP_076548232            2176 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 3 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548232
VERSION     XP_076548232.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692117.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2176
                     /product="rho guanine nucleotide exchange factor 3 isoform
                     X1"
                     /calculated_mol_wt=241758
     Region          <66..292
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1682..1734
                     /region_name="SH3_ARHGEF9_like"
                     /note="Src homology 3 domain of ARHGEF9-like Rho guanine
                     nucleotide exchange factors; cd11828"
                     /db_xref="CDD:212762"
     Site            order(1684..1686,1691..1692,1694,1730,1733)
                     /site_type="other"
                     /note="DH-PH interface [polypeptide binding]"
                     /db_xref="CDD:212762"
     Site            order(1687,1689,1692,1696,1714..1715,1728,1730..1731)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212762"
     Region          1777..1956
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; smart00325"
                     /db_xref="CDD:214619"
     Site            order(1780,1784,1882,1906..1907,1910..1911,1913..1914,
                     1917..1918,1921..1922,1925,1951,1955)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1961..2099
                     /region_name="PH_Collybistin_ASEF"
                     /note="Collybistin/APC-stimulated guanine nucleotide
                     exchange factor pleckstrin homology (PH) domain; cd01224"
                     /db_xref="CDD:269931"
     CDS             1..2176
                     /gene="RhoGEF3"
                     /coded_by="XM_076692117.1:1030..7560"
                     /db_xref="GeneID:117607240"
ORIGIN      
        1 mnrredpllr qhrsrllqla eatnikpgrg sgkrrgqrqs hgfgpsnggp sgggrvtlqd
       61 ivrsdpgytp niehlvfper kssnpnlakt sdespsnksk snptssgrsr laevfsrhys
      121 rgssqdssvt srknhlnsts ldsgatvshq atgsstgatv vtrsggrrwl sqssqstrqh
      181 saedgvrggn vgvigpvsts sssqapapal pprrvspads tdisntatgs rgdrgpnvsi
      241 lhlrgtadpw evgsqgstys igsnksqtgg kgkssgntrg sytrgtsips lkrhdttasa
      301 sstsslkqhr qdshgqvtss rrressnsfl ssnsgtsgel fisgdcsrel spvrwcdrev
      361 dgvylgrsgw vqvqqrslde nrrinyessl vtattgtlpl prratvklad yhcnsepgkc
      421 peeflrldsq rpdylalhgq dfdsvasstc tsphsipesy sppsitpiis pppafqdvig
      481 kkssskhssg rnnygkapfl prsnaivdsd iispppsppr qvnwsslpss srksagtpsr
      541 prrqnttsqq qqqqqqqqqy rmaqaksled qsstrrsqfv qrylesssss sssvgfrsld
      601 scvtrstmpr laentdssve gyddgddedd npssslnlsl vsssiialns snesirange
      661 rispnrqgrh hrsqqglrrs pgssesgkls fnspssssss sssaerqgrs ptpnpfrran
      721 atrqhqsart aqvspeshqs rvrrsrslql pekrspgaaa psrehsresn ephrvvvkis
      781 tdrgnerkrh ilqnrkaqst edalneellr eteavteyly gtrsraitrn lmpsrferre
      841 dsqeqrqrnn lttydvyfis skqqqpqssk ssgssmqqsq qqqqaqqqqq qqqqqqsrrp
      901 ktlqrgattp nanpsssnhd fcispeaklr cnsstcdfwp hcsqretlys psqapvfmkl
      961 sqsypahqrl ptdtgsshvn rgsassspas lermddrefr erensrknrv nreqnnpsts
     1021 kyqdrqpksv lkqpnrwspl egsngngsnv ekrehhrfyq kpeyagsfea cenkdrkvsp
     1081 vqgknivnrs psghvtssst ssssssdiwv ttsdrtvtks prnakssgas tpmedavigs
     1141 lktlieppkd gmlsrpgsap trgedslsad asldphqrsl slpksflthn tdsdkpatrk
     1201 fvgiedrssc lsisnnkgsw qrgqssqrss pspsrlhhtq eaatphtapv splhdqrsna
     1261 ysgkerrrip glskaqrrik asstpalldr dvesrqwsgd eedegttghv ttehplteat
     1321 iplvshsrlq eqpsnlsgni vtasgiqnpa rsstqsqqes vlqkfrksfs lrfhkkgske
     1381 snesecpved ndgsgpldee eerepsnvte qtsqhkedtn sdqkfrfgpl vwrsskerkk
     1441 gskaarnakc nsgdsgiqie mvsggaltgg ggggmigggd sseshdtdaq detdsppalr
     1501 rrvadksrpq selvnqilid kfkadlqsrt srhqhvrrtn sdlggqrllq wdtrsgyshv
     1561 hnyrrmlstp spiktrpprl sprhsshdiv tlrsnakgrs srtnlrrsls qplginqlsp
     1621 lmrtktagar lpggnvlsed eqdgrggtsd demmsdsess iasltdrkks feqtmdeevv
     1681 ilaeavwdhv amepeelafr agdvievldn ldkdwwwgsc rgehgwfpaa fvrlrvsqed
     1741 tvedclaama sggtsnsqlr rrtsvsllsn eqvrtsvvre lvqterdfvk ilrdvaegyi
     1801 aecrrrtdmf tedqietifi nleelldfqs eflkdlesri dwnspykscv gecflnhrag
     1861 frmyseycns hpmatatlqe lyqhnryskf feacrlmrgl ieipldgyll tpvqrickyp
     1921 lqlaellkyt kadhpdyhki qealeamrgv avlinerkrr mesleklaaw qlrvegwege
     1981 dlievssqli yqgdairvtt gmwtnnitlf lfdhqlvyck kdilkrntyv ykgriyldts
     2041 evidvpdgkd hqlgvtvrhc lkvyscvrdk wllfccrtae ekrrwleama eerrlvvqdr
     2101 ndglefpaaa kqlarlaatr lqnrppikpr nktykresay eiptmigqgt tnslgrkvgt
     2161 wftfgsskks trlqps
//
LOCUS       XP_034186585            2155 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 3 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186585
VERSION     XP_034186585.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330694.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186585.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2155
                     /product="rho guanine nucleotide exchange factor 3 isoform
                     X2"
                     /calculated_mol_wt=239466
     Region          <66..292
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1661..1713
                     /region_name="SH3_ARHGEF9_like"
                     /note="Src homology 3 domain of ARHGEF9-like Rho guanine
                     nucleotide exchange factors; cd11828"
                     /db_xref="CDD:212762"
     Site            order(1663..1665,1670..1671,1673,1709,1712)
                     /site_type="other"
                     /note="DH-PH interface [polypeptide binding]"
                     /db_xref="CDD:212762"
     Site            order(1666,1668,1671,1675,1693..1694,1707,1709..1710)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212762"
     Region          1756..1935
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; smart00325"
                     /db_xref="CDD:214619"
     Site            order(1759,1763,1861,1885..1886,1889..1890,1892..1893,
                     1896..1897,1900..1901,1904,1930,1934)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1940..2078
                     /region_name="PH_Collybistin_ASEF"
                     /note="Collybistin/APC-stimulated guanine nucleotide
                     exchange factor pleckstrin homology (PH) domain; cd01224"
                     /db_xref="CDD:269931"
     CDS             1..2155
                     /gene="RhoGEF3"
                     /coded_by="XM_034330694.2:809..7276"
                     /db_xref="GeneID:117607240"
ORIGIN      
        1 mnrredpllr qhrsrllqla eatnikpgrg sgkrrgqrqs hgfgpsnggp sgggrvtlqd
       61 ivrsdpgytp niehlvfper kssnpnlakt sdespsnksk snptssgrsr laevfsrhys
      121 rgssqdssvt srknhlnsts ldsgatvshq atgsstgatv vtrsggrrwl sqssqstrqh
      181 saedgvrggn vgvigpvsts sssqapapal pprrvspads tdisntatgs rgdrgpnvsi
      241 lhlrgtadpw evgsqgstys igsnksqtgg kgkssgntrg sytrgtsips lkrhdttasa
      301 sstsslkqhr qdshgqvtss rrressnsfl ssnsgtsgel fisgdcsrel spvrwcdrev
      361 dgvylgrsgw vqvqqrslde nrrinyessl vtattgtlpl prratvklad yhcnsepgkc
      421 peeflrldsq rpdylalhgq dfdsvasstc tsphsipesy sppsitpiis pppafqdvig
      481 kkssskhssg rnnygkapfl prsnaivdsd iispppsppr qvnwsslpss srksagtpsr
      541 prrqnttsqq qqqqqqqqqy rmaqaksled qsstrrsqfv qrylesssss sssvgfrsld
      601 scvtrstmpr laentdssve gyddgddedd npssslnlsl vsssiialns snesirange
      661 rispnrqgrh hrsqqglrrs pgssesgkls fnspssssss sssaerqgrs ptpnpfrran
      721 atrqhqsart aqvspeshqs rvrrsrslql pekrspgaaa psrehsresn ephrvvvkis
      781 tdrgnerkrh ilqnrkaqst edalneellr eteavteyly gtrsraitrn lmpsrferre
      841 dsqeqrqrnn lttydvyfis skqqqpqssk ssgssmqqsq qqqqaqqqqq qqqqqqsrrp
      901 ktlqrgattp nanpsssnhd fcispeaklr cnsstcdfwp hcsqretlys psqapvfmkl
      961 sqsypahqrl ptdtgsshvn rgsassspas lermddrefr erensrknrv nreqnnpsts
     1021 kyqdrqpksv lkqpnrwspl egsngngsnv ekrehhrfyq kpeyagsfea cenkdrkvsp
     1081 vqgknivnrs psghvtssst ssssssdiwv ttsdrtvtks prnakssgas tpmedavigs
     1141 lktlieppkd gmlsrpgsap trgedslsad asldphqrsl slpksflthn tdsnnkgswq
     1201 rgqssqrssp spsrlhhtqe aatphtapvs plhdqrsnay sgkerrripg lskaqrrika
     1261 sstpalldrd vesrqwsgde edegttghvt tehplteati plvshsrlqe qpsnlsgniv
     1321 tasgiqnpar sstqsqqesv lqkfrksfsl rfhkkgskes nesecpvedn dgsgpldeee
     1381 erepsnvteq tsqhkedtns dqkfrfgplv wrsskerkkg skaarnakcn sgdsgiqiem
     1441 vsggaltggg gggmigggds seshdtdaqd etdsppalrr rvadksrpqs elvnqilidk
     1501 fkadlqsrts rhqhvrrtns dlggqrllqw dtrsgyshvh nyrrmlstps piktrpprls
     1561 prhsshdivt lrsnakgrss rtnlrrslsq plginqlspl mrtktagarl pggnvlsede
     1621 qdgrggtsdd emmsdsessi asltdrkksf eqtmdeevvi laeavwdhva mepeelafra
     1681 gdvievldnl dkdwwwgscr gehgwfpaaf vrlrvsqedt vedclaamas ggtsnsqlrr
     1741 rtsvsllsne qvrtsvvrel vqterdfvki lrdvaegyia ecrrrtdmft edqietifin
     1801 leelldfqse flkdlesrid wnspykscvg ecflnhragf rmyseycnsh pmatatlqel
     1861 yqhnryskff eacrlmrgli eipldgyllt pvqrickypl qlaellkytk adhpdyhkiq
     1921 ealeamrgva vlinerkrrm esleklaawq lrvegweged lievssqliy qgdairvttg
     1981 mwtnnitlfl fdhqlvyckk dilkrntyvy kgriyldtse vidvpdgkdh qlgvtvrhcl
     2041 kvyscvrdkw llfccrtaee krrwleamae errlvvqdrn dglefpaaak qlarlaatrl
     2101 qnrppikprn ktykresaye iptmigqgtt nslgrkvgtw ftfgsskkst rlqps
//
LOCUS       XP_076548233            2113 aa            linear   INV 03-SEP-2025
DEFINITION  rho guanine nucleotide exchange factor 3 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548233
VERSION     XP_076548233.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692118.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..2113
                     /product="rho guanine nucleotide exchange factor 3 isoform
                     X3"
                     /calculated_mol_wt=235305
     Region          <66..292
                     /region_name="Herpes_BLLF1"
                     /note="Herpes virus major outer envelope glycoprotein
                     (BLLF1); pfam05109"
                     /db_xref="CDD:282904"
     Region          1619..1671
                     /region_name="SH3_ARHGEF9_like"
                     /note="Src homology 3 domain of ARHGEF9-like Rho guanine
                     nucleotide exchange factors; cd11828"
                     /db_xref="CDD:212762"
     Site            order(1621..1623,1628..1629,1631,1667,1670)
                     /site_type="other"
                     /note="DH-PH interface [polypeptide binding]"
                     /db_xref="CDD:212762"
     Site            order(1624,1626,1629,1633,1651..1652,1665,1667..1668)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212762"
     Region          1714..1893
                     /region_name="RhoGEF"
                     /note="Guanine nucleotide exchange factor for
                     Rho/Rac/Cdc42-like GTPases; smart00325"
                     /db_xref="CDD:214619"
     Site            order(1717,1721,1819,1843..1844,1847..1848,1850..1851,
                     1854..1855,1858..1859,1862,1888,1892)
                     /site_type="other"
                     /note="GTPase interaction site [polypeptide binding]"
                     /db_xref="CDD:238091"
     Region          1898..2036
                     /region_name="PH_Collybistin_ASEF"
                     /note="Collybistin/APC-stimulated guanine nucleotide
                     exchange factor pleckstrin homology (PH) domain; cd01224"
                     /db_xref="CDD:269931"
     CDS             1..2113
                     /gene="RhoGEF3"
                     /coded_by="XM_076692118.1:809..7150"
                     /db_xref="GeneID:117607240"
ORIGIN      
        1 mnrredpllr qhrsrllqla eatnikpgrg sgkrrgqrqs hgfgpsnggp sgggrvtlqd
       61 ivrsdpgytp niehlvfper kssnpnlakt sdespsnksk snptssgrsr laevfsrhys
      121 rgssqdssvt srknhlnsts ldsgatvshq atgsstgatv vtrsggrrwl sqssqstrqh
      181 saedgvrggn vgvigpvsts sssqapapal pprrvspads tdisntatgs rgdrgpnvsi
      241 lhlrgtadpw evgsqgstys igsnksqtgg kgkssgntrg sytrgtsips lkrhdttasa
      301 sstsslkqhr qdshgqvtss rrressnsfl ssnsgtsgel fisgdcsrel spvrwcdrev
      361 dgvylgrsgw vqvqqrslde nrrinyessl vtattgtlpl prratvklad yhcnsepgkc
      421 peeflrldsq rpdylalhgq dfdsvasstc tsphsipesy sppsitpiis pppafqdvig
      481 kkssskhssg rnnygkapfl prsnaivdsd iispppsppr qvnwsslpss srksagtpsr
      541 prrqnttsqq qqqqqqqqqy rmaqaksled qsstrrsqfv qrylesssss sssvgfrsld
      601 scvtrstmpr laentdssve gyddgddedd npssslnlsl vsssiialns snesirange
      661 rispnrqgrh hrsqqglrrs pgssesgkls fnspssssss sssaerqgrs ptpnpfrran
      721 atrqhqsart aqvspeshqs rvrrsrslql pekrspgaaa psrehsresn ephrvvvkis
      781 tdrgnerkrh ilqnrkaqst edalneellr eteavteyly gtrsraitrn lmpsrferre
      841 dsqeqrqrnn lttydvyfis skqqqpqssk ssgssmqqsq qqqqaqqqqq qqqqqqsrrp
      901 ktlqrgattp nanpsssnhd fcispeaklr cnsstcdfwp hcsqretlys psqapvfmkl
      961 sqsypahqrl ptdtgsshvn rgsassspas lermddrefr erensrknrv nreqnnpsts
     1021 kyqdrqpksv lkqpnrwspl egsngngsnv ekrehhrfyq kpeyagsfea cenkdrkvsp
     1081 vqgknivnrs psghvtssst ssssssdiwv ttsdrtvtks prnakssgas tpmedavigs
     1141 lktlieppkd gmlsrpgsap trgedslsad asldphqrsl slpksflthn tdsdkpatrk
     1201 fvgiedrssc lsisnnkgsw qrgqssqrss pspsrlhhtq eaatphtapv splhdqrsna
     1261 ysgkerrrip glskaqrrik asstpalldr dvesrqwsgd eedegttghv ttehplteat
     1321 iplvshsrlq eqpsnlsgni vtasgiqnpa rsstqsqqes vlqkfrksfs lrfhkkgske
     1381 snesecpved ndgsgpldee eerepsnvte qtsqhkedtn sdqkfrfgpl vwrsskerkk
     1441 gskaarnakc nsgdsgiqie adlqsrtsrh qhvrrtnsdl ggqrllqwdt rsgyshvhny
     1501 rrmlstpspi ktrpprlspr hsshdivtlr snakgrssrt nlrrslsqpl ginqlsplmr
     1561 tktagarlpg gnvlsedeqd grggtsddem msdsessias ltdrkksfeq tmdeevvila
     1621 eavwdhvame peelafragd vievldnldk dwwwgscrge hgwfpaafvr lrvsqedtve
     1681 dclaamasgg tsnsqlrrrt svsllsneqv rtsvvrelvq terdfvkilr dvaegyiaec
     1741 rrrtdmfted qietifinle elldfqsefl kdlesridwn spykscvgec flnhragfrm
     1801 yseycnshpm atatlqelyq hnryskffea crlmrgliei pldgylltpv qrickyplql
     1861 aellkytkad hpdyhkiqea leamrgvavl inerkrrmes leklaawqlr vegwegedli
     1921 evssqliyqg dairvttgmw tnnitlflfd hqlvyckkdi lkrntyvykg riyldtsevi
     1981 dvpdgkdhql gvtvrhclkv yscvrdkwll fccrtaeekr rwleamaeer rlvvqdrndg
     2041 lefpaaakql arlaatrlqn rppikprnkt ykresayeip tmigqgttns lgrkvgtwft
     2101 fgsskkstrl qps
//
LOCUS       XP_034186487            1349 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607202 [Osmia lignaria lignaria].
ACCESSION   XP_034186487
VERSION     XP_034186487.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330596.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186487.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1349
                     /product="uncharacterized protein LOC117607202"
                     /calculated_mol_wt=157626
     Region          45..63
                     /region_name="zf-CCCH"
                     /note="Zinc finger C-x8-C-x5-C-x3-H type (and similar);
                     pfam00642"
                     /db_xref="CDD:459885"
     Region          <316..>530
                     /region_name="PTZ00121"
                     /note="MAEBL; Provisional"
                     /db_xref="CDD:173412"
     CDS             1..1349
                     /gene="LOC117607202"
                     /coded_by="XM_034330596.2:1..4050"
                     /db_xref="GeneID:117607202"
ORIGIN      
        1 mskssirkit vdptkcntds rrpsvferlg tkpavtagqn tsdycrnwal ngscsygknc
       61 kyanthtlis pskrakkdna iasttglied pfkrltskiv kkashspdln leewnqtdle
      121 yedekvlerr rqllqrelel qmkkdkevhg kdkvkqkkka mtssssshts stssssssss
      181 sedsssssts dsrkkvkkik skrhhsgstd ydedkerrrk lklkrlgtkn ekpsvkkkrk
      241 fesssakkei tarsvkkyss sstsrkhsst prtrspavqv sapatpatav lgssvslphh
      301 gtsnkvkern rsespkatks rdidkeerhy kkirdvdels kdgtknkpfd kvkeqdkekn
      361 rtieeklkie erlklkykdk eirsrtppts ekskhqhskd tistktrrsr tpeksskskr
      421 eltpsklpdr pvstnrsrds ekkdreyhks dktkdredir kndqnnkarq isnqeeshrr
      481 igkecddkay lgdktrqksr errdkdhtrd erghsrlgrd qrdtrdkdke elqercrerp
      541 rerdrdrdrd rerdrdrerd rdrdrdrdrd rdrdrdrdrd rdrdrdrerd rdrdrdrere
      601 kdpiktrdrd ipslvsttig ltadknsrys rdkerivvkd safekngird rrgdrerers
      661 etktlsdrdr asqrydrntv dkeastrktg mnsprdrfpr ersldrvsll dkgvhnvppl
      721 tqasslpvvq ssiassssss apatlstsrd nlieridvgh ydrerhrrfn trydrghtse
      781 hdqsrvtptk vdrlierreg sprrtieidr nynrnygrls ehwdeqeeas shldyrdhhv
      841 heddrrktvd grrydspfde rrgireerpr esryvvqsgd rtsfddhrhh hhhhrhplyp
      901 gekirsntsi rdenvvnddw dshhrdvehn reraygpvdw eerewrvrtl wdsrdslprs
      961 evhdddwssr ydnsvsdwks ndgrkwdnqs vhirghyrnd rskeielges tshnkrrtyn
     1021 nsetrdectv hsskqaslyg smkeepinkk lmelaegkls ttreksadtf pkkvlpkeks
     1081 etkeivatep krscideslq tlhtesdlsd isddpddiln mddvtdidtn ktrtskktpd
     1141 itqrdgqstv qetevsspke piedtvfntk gkdnaepmsf rnmedenvet mdfeeisdge
     1201 leediktsgk glgdalgvdw eslvketqpr rptscnqsse nrwqckailh rigvsakyag
     1261 ediikkltkk ygkddhseff lhdvafmhta larnrllhdl nndmlptvdd flyrssnvni
     1321 dedvdydlev lkpctslyee akcllqqvv
//
LOCUS       XP_034186490            1098 aa            linear   INV 03-SEP-2025
DEFINITION  multiple edematous wings isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186490
VERSION     XP_034186490.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330599.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1098
                     /product="multiple edematous wings isoform X1"
                     /calculated_mol_wt=122915
     Region          33..97
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          308..>348
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          372..422
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          434..>456
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          489..961
                     /region_name="Integrin_alpha2"
                     /note="Integrin alpha; pfam08441"
                     /db_xref="CDD:462478"
     CDS             1..1098
                     /gene="mew"
                     /coded_by="XM_034330599.2:1..3297"
                     /db_xref="GeneID:117607204"
ORIGIN      
        1 mknyqltwfi lniylvytfn lepripvikk glngsyfgys vaehqeipdd ntekpkswml
       61 igapldqnrq pstnrsgalw qcplttfktd ctqvitdgrl teegglydts fesdnlvppg
      121 ndeikdnqwl gvtvrsqgig gkvmvcahrh vvktadsqwg qgqcyiltqd lkyqdlkkpc
      181 sgkptnkahe qfgycqagts gvltsedrvv igtpgphtwr gtlylftisd efltrdntvy
      241 napmqdaspv skysylgmsv tvgnffgtgl ayaagaprsn gtgqvilltr rdfkpdmdva
      301 fildgeqfas sfgyeitsad vngdkvtdli vaapfyfskv eggavyvyts lqktykegek
      361 skpvklvgre esrfgfaltn lgdlnkdgye diavgapyek kgtvyiylgs kngiitipsq
      421 vihaddmpep lqtfgyslsg gidmdqngya dllvgayend avallrskki idittyiryl
      481 kkdgtyqeki epidpnkvgc pedpnsnhtc fsfeacckte slvkeddmqn lklnyyieae
      541 tytgvkkfsr vwfggghsrl hyinrtvild ttklehcqke tiylkentrd iqspikfrln
      601 ysliqeepim paegeslpdi knypilnqqe aarvfdatfq kdcgnndice sdlqvqarln
      661 lsassvkpnf yelllgeree vvvdinvsni gesayeaqlf ivhspslnyi asksndsisc
      721 nlhnssliac signpfkkdk llniqvrfdp kelednksql vfvifansts keikekepvk
      781 lqatvvkrae lsikgsakiq waryggpvig esaikylnev gpkvshvyqv fnegpwrvss
      841 levriswpye vandkahgkw llyleelpii qgsgdsecil ppghvvnplk lqnstnyddl
      901 dglqstvstp tlhnhtnhlr tkrdtekvvn trtiidkdgh rhqvvsmnck agtakcfdit
      961 cfiynlqrkq eamitvkarl wnstlvedyp kvdqvkissn akivippnvv iqqenlkddy
     1021 avaetivhpd lldhqdvepv piwviivavv aglilfillt lvlrklgffk rrrpdptlsg
     1081 nlekhkddnp esealfkr
//
LOCUS       XP_034186491            1097 aa            linear   INV 03-SEP-2025
DEFINITION  multiple edematous wings isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186491
VERSION     XP_034186491.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330600.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1097
                     /product="multiple edematous wings isoform X2"
                     /calculated_mol_wt=122813
     Region          33..97
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          307..>347
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          371..421
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          433..>455
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          488..960
                     /region_name="Integrin_alpha2"
                     /note="Integrin alpha; pfam08441"
                     /db_xref="CDD:462478"
     CDS             1..1097
                     /gene="mew"
                     /coded_by="XM_034330600.2:1..3294"
                     /db_xref="GeneID:117607204"
ORIGIN      
        1 mknyqltwfi lniylvytfn lepripvikk glngsyfgys vaehqeipdd ntekpkswml
       61 igapldqnrq pstnrsgalw qcplttfktd ctqvitdgrl kegglydtsf esdnlvppgn
      121 deikdnqwlg vtvrsqgigg kvmvcahrhv vktadsqwgq gqcyiltqdl kyqdlkkpcs
      181 gkptnkaheq fgycqagtsg vltsedrvvi gtpgphtwrg tlylftisde fltrdntvyn
      241 apmqdaspvs kysylgmsvt vgnffgtgla yaagaprsng tgqvilltrr dfkpdmdvaf
      301 ildgeqfass fgyeitsadv ngdkvtdliv aapfyfskve ggavyvytsl qktykegeks
      361 kpvklvgree srfgfaltnl gdlnkdgyed iavgapyekk gtvyiylgsk ngiitipsqv
      421 ihaddmpepl qtfgyslsgg idmdqngyad llvgayenda vallrskkii dittyirylk
      481 kdgtyqekie pidpnkvgcp edpnsnhtcf sfeaccktes lvkeddmqnl klnyyieaet
      541 ytgvkkfsrv wfggghsrlh yinrtvildt tklehcqket iylkentrdi qspikfrlny
      601 sliqeepimp aegeslpdik nypilnqqea arvfdatfqk dcgnndices dlqvqarlnl
      661 sassvkpnfy elllgereev vvdinvsnig esayeaqlfi vhspslnyia sksndsiscn
      721 lhnssliacs ignpfkkdkl lniqvrfdpk elednksqlv fvifanstsk eikekepvkl
      781 qatvvkrael sikgsakiqw aryggpvige saikylnevg pkvshvyqvf negpwrvssl
      841 evriswpyev andkahgkwl lyleelpiiq gsgdsecilp pghvvnplkl qnstnyddld
      901 glqstvstpt lhnhtnhlrt krdtekvvnt rtiidkdghr hqvvsmncka gtakcfditc
      961 fiynlqrkqe amitvkarlw nstlvedypk vdqvkissna kivippnvvi qqenlkddya
     1021 vaetivhpdl ldhqdvepvp iwviivavva glilfilltl vlrklgffkr rrpdptlsgn
     1081 lekhkddnpe sealfkr
//
LOCUS       XP_034186492            1089 aa            linear   INV 03-SEP-2025
DEFINITION  multiple edematous wings isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_034186492
VERSION     XP_034186492.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330601.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1089
                     /product="multiple edematous wings isoform X3"
                     /calculated_mol_wt=121949
     Region          33..97
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          299..>339
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          363..413
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          425..>447
                     /region_name="Int_alpha"
                     /note="Integrin alpha (beta-propellor repeats);
                     smart00191"
                     /db_xref="CDD:214549"
     Region          480..952
                     /region_name="Integrin_alpha2"
                     /note="Integrin alpha; pfam08441"
                     /db_xref="CDD:462478"
     CDS             1..1089
                     /gene="mew"
                     /coded_by="XM_034330601.2:1..3270"
                     /db_xref="GeneID:117607204"
ORIGIN      
        1 mknyqltwfi lniylvytfn lepripvikk glngsyfgys vaehqeipdd ntekpkswml
       61 igapldqnrq pstnrsgalw qcplttfktd ctqvitdgrl sfesdnlvpp gndeikdnqw
      121 lgvtvrsqgi ggkvmvcahr hvvktadsqw gqgqcyiltq dlkyqdlkkp csgkptnkah
      181 eqfgycqagt sgvltsedrv vigtpgphtw rgtlylftis defltrdntv ynapmqdasp
      241 vskysylgms vtvgnffgtg layaagaprs ngtgqvillt rrdfkpdmdv afildgeqfa
      301 ssfgyeitsa dvngdkvtdl ivaapfyfsk veggavyvyt slqktykege kskpvklvgr
      361 eesrfgfalt nlgdlnkdgy ediavgapye kkgtvyiylg skngiitips qvihaddmpe
      421 plqtfgysls ggidmdqngy adllvgayen davallrskk iidittyiry lkkdgtyqek
      481 iepidpnkvg cpedpnsnht cfsfeacckt eslvkeddmq nlklnyyiea etytgvkkfs
      541 rvwfggghsr lhyinrtvil dttklehcqk etiylkentr diqspikfrl nysliqeepi
      601 mpaegeslpd iknypilnqq eaarvfdatf qkdcgnndic esdlqvqarl nlsassvkpn
      661 fyelllgere evvvdinvsn igesayeaql fivhspslny iasksndsis cnlhnsslia
      721 csignpfkkd kllniqvrfd pkelednksq lvfvifanst skeikekepv klqatvvkra
      781 elsikgsaki qwaryggpvi gesaikylne vgpkvshvyq vfnegpwrvs slevriswpy
      841 evandkahgk wllyleelpi iqgsgdseci lppghvvnpl klqnstnydd ldglqstvst
      901 ptlhnhtnhl rtkrdtekvv ntrtiidkdg hrhqvvsmnc kagtakcfdi tcfiynlqrk
      961 qeamitvkar lwnstlvedy pkvdqvkiss nakivippnv viqqenlkdd yavaetivhp
     1021 dlldhqdvep vpiwviivav vaglilfill tlvlrklgff krrrpdptls gnlekhkddn
     1081 pesealfkr
//
LOCUS       XP_076548234            1018 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548234
VERSION     XP_076548234.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692119.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1018
                     /product="disks large 1 tumor suppressor protein isoform
                     X1"
                     /calculated_mol_wt=113169
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          826..1004
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1018
                     /gene="LOC117607241"
                     /coded_by="XM_076692119.1:331..3387"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk
      721 kknfsfsrkf pfmkskddks edgsdqersp ttpenendpt pfmlcytqdd tntegsreil
      781 yrvelpyiee ltlvaleked denndelsse envlsyeavq qltiqytrpv iilgplkdri
      841 nddlisefpd kfgscvphtt rsrreyevdg rdyhfvasre qmerdiqnhl fieagqyndn
      901 lygtsvasvr evaekgkhci ldvsgnaikr lqvaqlypia ifikpksves vmemnkrmte
      961 eqakktyera lkmeqefgey ftavvqgdtp eeiyvkvkev iaeqsgpniw vpcrdqql
//
LOCUS       XP_076548235            1018 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548235
VERSION     XP_076548235.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692120.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1018
                     /product="disks large 1 tumor suppressor protein isoform
                     X2"
                     /calculated_mol_wt=113172
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          826..1004
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1018
                     /gene="LOC117607241"
                     /coded_by="XM_076692120.1:53..3109"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mwlpvisilw aivnlmqrkl tpngavystl detaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk
      721 kknfsfsrkf pfmkskddks edgsdqersp ttpenendpt pfmlcytqdd tntegsreil
      781 yrvelpyiee ltlvaleked denndelsse envlsyeavq qltiqytrpv iilgplkdri
      841 nddlisefpd kfgscvphtt rsrreyevdg rdyhfvasre qmerdiqnhl fieagqyndn
      901 lygtsvasvr evaekgkhci ldvsgnaikr lqvaqlypia ifikpksves vmemnkrmte
      961 eqakktyera lkmeqefgey ftavvqgdtp eeiyvkvkev iaeqsgpniw vpcrdqql
//
LOCUS       XP_076548236            1017 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548236
VERSION     XP_076548236.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692121.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1017
                     /product="disks large 1 tumor suppressor protein isoform
                     X3"
                     /calculated_mol_wt=113041
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..430
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,411,415,418..419)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          501..591
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(513..519,530,533,563,567,570..571)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          622..682
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(624,646..647)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(627,629,632,641,659..660,679,681..682)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          825..1003
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1017
                     /gene="LOC117607241"
                     /coded_by="XM_076692121.1:330..3383"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lgdknlenvt heeavatlka
      421 tqdrvvllva kpetgivppp pppmasdnsl spqprkqngs vsalennstl pysqesrhas
      481 slalhgaatp ravsqedvsr evrtvvlnkg ssglgfnivg gedgegifis filaggpadl
      541 sgelrrgdqi lsvnginlrt atheeaaaal kgtgqtvtiv vqykpedynr feakihdlkq
      601 qisqqnmmtg tlmrtsqkks lyvralfdyd pnkddglpsr glafrygeil hvtnasddew
      661 wqarrvtpqg deeglgiips rrrwerkqra rdrsvkfqgh mpvildkidg lqqstldrkk
      721 knfsfsrkfp fmkskddkse dgsdqerspt tpenendptp fmlcytqddt ntegsreily
      781 rvelpyieel tlvalekedd enndelssee nvlsyeavqq ltiqytrpvi ilgplkdrin
      841 ddlisefpdk fgscvphttr srreyevdgr dyhfvasreq merdiqnhlf ieagqyndnl
      901 ygtsvasvre vaekgkhcil dvsgnaikrl qvaqlypiai fikpksvesv memnkrmtee
      961 qakktyeral kmeqefgeyf tavvqgdtpe eiyvkvkevi aeqsgpniwv pcrdqql
//
LOCUS       XP_076548237            1017 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548237
VERSION     XP_076548237.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692122.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1017
                     /product="disks large 1 tumor suppressor protein isoform
                     X4"
                     /calculated_mol_wt=113112
     Region          34..91
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          246..334
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(257..263,280,283,313,317,320..321)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          341..430
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(350..356,373,376,411,415,418..419)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          501..591
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(513..519,530,533,563,567,570..571)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          622..682
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(624,646..647)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(627,629,632,641,659..660,679,681..682)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          825..1003
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1017
                     /gene="LOC117607241"
                     /coded_by="XM_076692122.1:331..3384"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqgefd qffgcdntdi itdfaprpar klaeahrale lledyhaklt rpqdkqlrla
       61 iervirifks rlfqalldiq efyeltllde sksvqqktae tiriadkwea sdgpitptfa
      121 qndggpafpv nqtlsniygr rsaspdvadh vdetqkpryt gggveeqlpp ppsppqlkea
      181 gqldgvprpn svdrilhpdg sqhiltshea lnkshdswqg hdgdqahtpl laadtrhvng
      241 dddweyeeiv lerggaglgf siaggtdnph fgndtaiyit klipggaasa dgrlrvndti
      301 lqvndvsvvd vphaaavdal kragntvkly vrrrrhtqli eielikgnkg lgfsiaggig
      361 nqhipgdngi yvtkimegga aqvdgrlvvg dklvavrnal qgdknlenvt heeavatlka
      421 tqdrvvllva kpetgivppp pppmasdnsl spqprkqngs vsalennstl pysqesrhas
      481 slalhgaatp ravsqedvsr evrtvvlnkg ssglgfnivg gedgegifis filaggpadl
      541 sgelrrgdqi lsvnginlrt atheeaaaal kgtgqtvtiv vqykpedynr feakihdlkq
      601 qisqqnmmtg tlmrtsqkks lyvralfdyd pnkddglpsr glafrygeil hvtnasddew
      661 wqarrvtpqg deeglgiips rrrwerkqra rdrsvkfqgh mpvildkidg lqqstldrkk
      721 knfsfsrkfp fmkskddkse dgsdqerspt tpenendptp fmlcytqddt ntegsreily
      781 rvelpyieel tlvalekedd enndelssee nvlsyeavqq ltiqytrpvi ilgplkdrin
      841 ddlisefpdk fgscvphttr srreyevdgr dyhfvasreq merdiqnhlf ieagqyndnl
      901 ygtsvasvre vaekgkhcil dvsgnaikrl qvaqlypiai fikpksvesv memnkrmtee
      961 qakktyeral kmeqefgeyf tavvqgdtpe eiyvkvkevi aeqsgpniwv pcrdqql
//
LOCUS       XP_076548238            1013 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548238
VERSION     XP_076548238.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692123.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1013
                     /product="disks large 1 tumor suppressor protein isoform
                     X5"
                     /calculated_mol_wt=112643
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          821..999
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1013
                     /gene="LOC117607241"
                     /coded_by="XM_076692123.1:328..3369"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkqs tldrkkknfs
      721 fsrkfpfmks kddksedgsd qerspttpen endptpfmlc ytqddtnteg sreilyrvel
      781 pyieeltlva lekeddennd elsseenvls yeavqqltiq ytrpviilgp lkdrinddli
      841 sefpdkfgsc vphttrsrre yevdgrdyhf vasreqmerd iqnhlfieag qyndnlygts
      901 vasvrevaek gkhcildvsg naikrlqvaq lypiaifikp ksvesvmemn krmteeqakk
      961 tyeralkmeq efgeyftavv qgdtpeeiyv kvkeviaeqs gpniwvpcrd qql
//
LOCUS       XP_076548239            1012 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548239
VERSION     XP_076548239.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692124.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1012
                     /product="disks large 1 tumor suppressor protein isoform
                     X6"
                     /calculated_mol_wt=112514
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..430
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,411,415,418..419)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          501..591
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(513..519,530,533,563,567,570..571)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          622..682
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(624,646..647)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(627,629,632,641,659..660,679,681..682)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          820..998
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1012
                     /gene="LOC117607241"
                     /coded_by="XM_076692124.1:327..3365"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lgdknlenvt heeavatlka
      421 tqdrvvllva kpetgivppp pppmasdnsl spqprkqngs vsalennstl pysqesrhas
      481 slalhgaatp ravsqedvsr evrtvvlnkg ssglgfnivg gedgegifis filaggpadl
      541 sgelrrgdqi lsvnginlrt atheeaaaal kgtgqtvtiv vqykpedynr feakihdlkq
      601 qisqqnmmtg tlmrtsqkks lyvralfdyd pnkddglpsr glafrygeil hvtnasddew
      661 wqarrvtpqg deeglgiips rrrwerkqra rdrsvkfqgh mpvildkqst ldrkkknfsf
      721 srkfpfmksk ddksedgsdq erspttpene ndptpfmlcy tqddtntegs reilyrvelp
      781 yieeltlval ekeddennde lsseenvlsy eavqqltiqy trpviilgpl kdrinddlis
      841 efpdkfgscv phttrsrrey evdgrdyhfv asreqmerdi qnhlfieagq yndnlygtsv
      901 asvrevaekg khcildvsgn aikrlqvaql ypiaifikpk svesvmemnk rmteeqakkt
      961 yeralkmeqe fgeyftavvq gdtpeeiyvk vkeviaeqsg pniwvpcrdq ql
//
LOCUS       XP_034186599            1008 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186599
VERSION     XP_034186599.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330708.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1008
                     /product="disks large 1 tumor suppressor protein isoform
                     X7"
                     /calculated_mol_wt=112209
     Region          26..82
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          237..325
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(248..254,271,274,304,308,311..312)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          332..421
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(341..347,364,367,402,406,409..410)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          492..582
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(504..510,521,524,554,558,561..562)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          613..673
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(615,637..638)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(618,620,623,632,650..651,670,672..673)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          816..994
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1008
                     /gene="LOC117607241"
                     /coded_by="XM_034330708.2:55..3081"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 myahvhgdng mccylptvfy leryqahral elledyhakl trpqdkqlrl aiervirifk
       61 srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf aqndggpafp
      121 vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke agqldgvprp
      181 nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn gdddweyeei
      241 vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt ilqvndvsvv
      301 dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi gnqhipgdng
      361 iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk atqdrvvllv
      421 akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha sslalhgaat
      481 pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad lsgelrrgdq
      541 ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk qqisqqnmmt
      601 gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde wwqarrvtpq
      661 gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk kknfsfsrkf
      721 pfmkskddks edgsdqersp ttpenendpt pfmlcytqdd tntegsreil yrvelpyiee
      781 ltlvaleked denndelsse envlsyeavq qltiqytrpv iilgplkdri nddlisefpd
      841 kfgscvphtt rsrreyevdg rdyhfvasre qmerdiqnhl fieagqyndn lygtsvasvr
      901 evaekgkhci ldvsgnaikr lqvaqlypia ifikpksves vmemnkrmte eqakktyera
      961 lkmeqefgey ftavvqgdtp eeiyvkvkev iaeqsgpniw vpcrdqql
//
LOCUS       XP_076548240            1006 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548240
VERSION     XP_076548240.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692125.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1006
                     /product="disks large 1 tumor suppressor protein isoform
                     X8"
                     /calculated_mol_wt=111885
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          235..323
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(246..252,269,272,302,306,309..310)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          330..419
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(339..345,362,365,400,404,407..408)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          490..580
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(502..508,519,522,552,556,559..560)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          611..671
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(613,635..636)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(616,618,621,630,648..649,668,670..671)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          814..992
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1006
                     /gene="LOC117607241"
                     /coded_by="XM_076692125.1:332..3352"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqvngd ddweyeeivl
      241 erggaglgfs iaggtdnphf gndtaiyitk lipggaasad grlrvndtil qvndvsvvdv
      301 phaaavdalk ragntvklyv rrrrhtqlie ielikgnkgl gfsiaggign qhipgdngiy
      361 vtkimeggaa qvdgrlvvgd klvavrnalq gdknlenvth eeavatlkat qdrvvllvak
      421 petgivpppp ppmasdnsls pqprkqngsv salennstlp ysqesrhass lalhgaatpr
      481 avsqedvsre vrtvvlnkgs sglgfnivgg edgegifisf ilaggpadls gelrrgdqil
      541 svnginlrta theeaaaalk gtgqtvtivv qykpedynrf eakihdlkqq isqqnmmtgt
      601 lmrtsqkksl yvralfdydp nkddglpsrg lafrygeilh vtnasddeww qarrvtpqgd
      661 eeglgiipsr rrwerkqrar drsvkfqghm pvildkidgl qqstldrkkk nfsfsrkfpf
      721 mkskddksed gsdqersptt penendptpf mlcytqddtn tegsreilyr velpyieelt
      781 lvalekedde nndelsseen vlsyeavqql tiqytrpvii lgplkdrind dlisefpdkf
      841 gscvphttrs rreyevdgrd yhfvasreqm erdiqnhlfi eagqyndnly gtsvasvrev
      901 aekgkhcild vsgnaikrlq vaqlypiaif ikpksvesvm emnkrmteeq akktyeralk
      961 meqefgeyft avvqgdtpee iyvkvkevia eqsgpniwvp crdqql
//
LOCUS       XP_076548241            1005 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548241
VERSION     XP_076548241.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692126.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1005
                     /product="disks large 1 tumor suppressor protein isoform
                     X9"
                     /calculated_mol_wt=111730
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          813..991
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1005
                     /gene="LOC117607241"
                     /coded_by="XM_076692126.1:330..3347"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk
      721 kknfsfsrkf pfmkskddks edgsdqepfm lcytqddtnt egsreilyrv elpyieeltl
      781 valekedden ndelsseenv lsyeavqqlt iqytrpviil gplkdrindd lisefpdkfg
      841 scvphttrsr reyevdgrdy hfvasreqme rdiqnhlfie agqyndnlyg tsvasvreva
      901 ekgkhcildv sgnaikrlqv aqlypiaifi kpksvesvme mnkrmteeqa kktyeralkm
      961 eqefgeyfta vvqgdtpeei yvkvkeviae qsgpniwvpc rdqql
//
LOCUS       XP_076548242            1000 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X10 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548242
VERSION     XP_076548242.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692127.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1000
                     /product="disks large 1 tumor suppressor protein isoform
                     X10"
                     /calculated_mol_wt=111203
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          808..986
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..1000
                     /gene="LOC117607241"
                     /coded_by="XM_076692127.1:327..3329"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkqs tldrkkknfs
      721 fsrkfpfmks kddksedgsd qepfmlcytq ddtntegsre ilyrvelpyi eeltlvalek
      781 eddenndels seenvlsyea vqqltiqytr pviilgplkd rinddlisef pdkfgscvph
      841 ttrsrreyev dgrdyhfvas reqmerdiqn hlfieagqyn dnlygtsvas vrevaekgkh
      901 cildvsgnai krlqvaqlyp iaifikpksv esvmemnkrm teeqakktye ralkmeqefg
      961 eyftavvqgd tpeeiyvkvk eviaeqsgpn iwvpcrdqql
//
LOCUS       XP_076548243             991 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X11 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548243
VERSION     XP_076548243.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692128.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..991
                     /product="disks large 1 tumor suppressor protein isoform
                     X11"
                     /calculated_mol_wt=110142
     Region          8..65
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          220..308
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(231..237,254,257,287,291,294..295)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          315..404
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(324..330,347,350,385,389,392..393)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          475..565
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(487..493,504,507,537,541,544..545)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          596..656
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(598,620..621)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(601,603,606,615,633..634,653,655..656)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          799..977
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..991
                     /gene="LOC117607241"
                     /coded_by="XM_076692128.1:330..3305"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqgeah ralelledyh akltrpqdkq lrlaiervir ifksrlfqal ldiqefyelt
       61 lldesksvqq ktaetiriad kweasdgpit ptfaqndggp afpvnqtlsn iygrrsaspd
      121 vadhvdetqk prytgggvee qlppppsppq lkeagqldgv prpnsvdril hpdgsqhilt
      181 shealnkshd swqghdgdqa htpllaadtr hvngdddwey eeivlergga glgfsiaggt
      241 dnphfgndta iyitklipgg aasadgrlrv ndtilqvndv svvdvphaaa vdalkragnt
      301 vklyvrrrrh tqlieielik gnkglgfsia ggignqhipg dngiyvtkim eggaaqvdgr
      361 lvvgdklvav rnalqgdknl envtheeava tlkatqdrvv llvakpetgi vppppppmas
      421 dnslspqprk qngsvsalen nstlpysqes rhasslalhg aatpravsqe dvsrevrtvv
      481 lnkgssglgf nivggedgeg ifisfilagg padlsgelrr gdqilsvngi nlrtatheea
      541 aaalkgtgqt vtivvqykpe dynrfeakih dlkqqisqqn mmtgtlmrts qkkslyvral
      601 fdydpnkddg lpsrglafry geilhvtnas ddewwqarrv tpqgdeeglg iipsrrrwer
      661 kqrardrsvk fqghmpvild kidglqqstl drkkknfsfs rkfpfmkskd dksedgsdqe
      721 rspttpenen dptpfmlcyt qddtntegsr eilyrvelpy ieeltlvale keddenndel
      781 sseenvlsye avqqltiqyt rpviilgplk drinddlise fpdkfgscvp httrsrreye
      841 vdgrdyhfva sreqmerdiq nhlfieagqy ndnlygtsva svrevaekgk hcildvsgna
      901 ikrlqvaqly piaifikpks vesvmemnkr mteeqakkty eralkmeqef geyftavvqg
      961 dtpeeiyvkv keviaeqsgp niwvpcrdqq l
//
LOCUS       XP_076548244             990 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X12 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548244
VERSION     XP_076548244.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692129.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..990
                     /product="disks large 1 tumor suppressor protein isoform
                     X12"
                     /calculated_mol_wt=110085
     Region          6..64
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          219..307
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(230..236,253,256,286,290,293..294)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          314..403
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(323..329,346,349,384,388,391..392)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          474..564
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(486..492,503,506,536,540,543..544)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          595..655
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(597,619..620)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(600,602,605,614,632..633,652,654..655)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          798..976
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..990
                     /gene="LOC117607241"
                     /coded_by="XM_076692129.1:329..3301"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqeahr alelledyha kltrpqdkql rlaierviri fksrlfqall diqefyeltl
       61 ldesksvqqk taetiriadk weasdgpitp tfaqndggpa fpvnqtlsni ygrrsaspdv
      121 adhvdetqkp rytgggveeq lppppsppql keagqldgvp rpnsvdrilh pdgsqhilts
      181 healnkshds wqghdgdqah tpllaadtrh vngdddweye eivlerggag lgfsiaggtd
      241 nphfgndtai yitklipgga asadgrlrvn dtilqvndvs vvdvphaaav dalkragntv
      301 klyvrrrrht qlieielikg nkglgfsiag gignqhipgd ngiyvtkime ggaaqvdgrl
      361 vvgdklvavr nalqgdknle nvtheeavat lkatqdrvvl lvakpetgiv ppppppmasd
      421 nslspqprkq ngsvsalenn stlpysqesr hasslalhga atpravsqed vsrevrtvvl
      481 nkgssglgfn ivggedgegi fisfilaggp adlsgelrrg dqilsvngin lrtatheeaa
      541 aalkgtgqtv tivvqykped ynrfeakihd lkqqisqqnm mtgtlmrtsq kkslyvralf
      601 dydpnkddgl psrglafryg eilhvtnasd dewwqarrvt pqgdeeglgi ipsrrrwerk
      661 qrardrsvkf qghmpvildk idglqqstld rkkknfsfsr kfpfmkskdd ksedgsdqer
      721 spttpenend ptpfmlcytq ddtntegsre ilyrvelpyi eeltlvalek eddenndels
      781 seenvlsyea vqqltiqytr pviilgplkd rinddlisef pdkfgscvph ttrsrreyev
      841 dgrdyhfvas reqmerdiqn hlfieagqyn dnlygtsvas vrevaekgkh cildvsgnai
      901 krlqvaqlyp iaifikpksv esvmemnkrm teeqakktye ralkmeqefg eyftavvqgd
      961 tpeeiyvkvk eviaeqsgpn iwvpcrdqql
//
LOCUS       XP_076548245             986 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X13 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548245
VERSION     XP_076548245.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692130.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..986
                     /product="disks large 1 tumor suppressor protein isoform
                     X13"
                     /calculated_mol_wt=109788
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          215..303
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(226..232,249,252,282,286,289..290)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          310..399
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(319..325,342,345,380,384,387..388)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          470..560
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(482..488,499,502,532,536,539..540)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          591..651
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(593,615..616)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(596,598,601,610,628..629,648,650..651)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          794..972
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..986
                     /gene="LOC117607241"
                     /coded_by="XM_076692130.1:331..3291"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndkprytg ggveeqlppp psppqlkeag qldgvprpns vdrilhpdgs qhiltsheal
      181 nkshdswqgh dgdqahtpll aadtrhvngd ddweyeeivl erggaglgfs iaggtdnphf
      241 gndtaiyitk lipggaasad grlrvndtil qvndvsvvdv phaaavdalk ragntvklyv
      301 rrrrhtqlie ielikgnkgl gfsiaggign qhipgdngiy vtkimeggaa qvdgrlvvgd
      361 klvavrnalq gdknlenvth eeavatlkat qdrvvllvak petgivpppp ppmasdnsls
      421 pqprkqngsv salennstlp ysqesrhass lalhgaatpr avsqedvsre vrtvvlnkgs
      481 sglgfnivgg edgegifisf ilaggpadls gelrrgdqil svnginlrta theeaaaalk
      541 gtgqtvtivv qykpedynrf eakihdlkqq isqqnmmtgt lmrtsqkksl yvralfdydp
      601 nkddglpsrg lafrygeilh vtnasddeww qarrvtpqgd eeglgiipsr rrwerkqrar
      661 drsvkfqghm pvildkidgl qqstldrkkk nfsfsrkfpf mkskddksed gsdqersptt
      721 penendptpf mlcytqddtn tegsreilyr velpyieelt lvalekedde nndelsseen
      781 vlsyeavqql tiqytrpvii lgplkdrind dlisefpdkf gscvphttrs rreyevdgrd
      841 yhfvasreqm erdiqnhlfi eagqyndnly gtsvasvrev aekgkhcild vsgnaikrlq
      901 vaqlypiaif ikpksvesvm emnkrmteeq akktyeralk meqefgeyft avvqgdtpee
      961 iyvkvkevia eqsgpniwvp crdqql
//
LOCUS       XP_076548246             985 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X14 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548246
VERSION     XP_076548246.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692131.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..985
                     /product="disks large 1 tumor suppressor protein isoform
                     X14"
                     /calculated_mol_wt=109249
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          793..971
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..985
                     /gene="LOC117607241"
                     /coded_by="XM_076692131.1:332..3289"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk
      721 kknfsfsrkf pfmkskddks edgsdqersp ttpenendpt pfmlcytqdd tntegseenv
      781 lsyeavqqlt iqytrpviil gplkdrindd lisefpdkfg scvphttrsr reyevdgrdy
      841 hfvasreqme rdiqnhlfie agqyndnlyg tsvasvreva ekgkhcildv sgnaikrlqv
      901 aqlypiaifi kpksvesvme mnkrmteeqa kktyeralkm eqefgeyfta vvqgdtpeei
      961 yvkvkeviae qsgpniwvpc rdqql
//
LOCUS       XP_076548247             972 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X15 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548247
VERSION     XP_076548247.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692132.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..972
                     /product="disks large 1 tumor suppressor protein isoform
                     X15"
                     /calculated_mol_wt=107809
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          780..958
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..972
                     /gene="LOC117607241"
                     /coded_by="XM_076692132.1:331..3249"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk
      721 kknfsfsrkf pfmkskddks edgsdqepfm lcytqddtnt egseenvlsy eavqqltiqy
      781 trpviilgpl kdrinddlis efpdkfgscv phttrsrrey evdgrdyhfv asreqmerdi
      841 qnhlfieagq yndnlygtsv asvrevaekg khcildvsgn aikrlqvaql ypiaifikpk
      901 svesvmemnk rmteeqakkt yeralkmeqe fgeyftavvq gdtpeeiyvk vkeviaeqsg
      961 pniwvpcrdq ql
//
LOCUS       XP_076548248             971 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X16 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548248
VERSION     XP_076548248.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692133.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..971
                     /product="disks large 1 tumor suppressor protein isoform
                     X16"
                     /calculated_mol_wt=107688
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          779..957
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..971
                     /gene="LOC117607241"
                     /coded_by="XM_076692133.1:332..3247"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk
      721 kknfsfsrkf pfmkskddks edgsdqersp ttpenendpt rseenvlsye avqqltiqyt
      781 rpviilgplk drinddlise fpdkfgscvp httrsrreye vdgrdyhfva sreqmerdiq
      841 nhlfieagqy ndnlygtsva svrevaekgk hcildvsgna ikrlqvaqly piaifikpks
      901 vesvmemnkr mteeqakkty eralkmeqef geyftavvqg dtpeeiyvkv keviaeqsgp
      961 niwvpcrdqq l
//
LOCUS       XP_076548249             958 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X17 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548249
VERSION     XP_076548249.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692134.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..958
                     /product="disks large 1 tumor suppressor protein isoform
                     X17"
                     /calculated_mol_wt=106249
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          766..944
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..958
                     /gene="LOC117607241"
                     /coded_by="XM_076692134.1:331..3207"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkid glqqstldrk
      721 kknfsfsrkf pfmkskddks edgsdqerse envlsyeavq qltiqytrpv iilgplkdri
      781 nddlisefpd kfgscvphtt rsrreyevdg rdyhfvasre qmerdiqnhl fieagqyndn
      841 lygtsvasvr evaekgkhci ldvsgnaikr lqvaqlypia ifikpksves vmemnkrmte
      901 eqakktyera lkmeqefgey ftavvqgdtp eeiyvkvkev iaeqsgpniw vpcrdqql
//
LOCUS       XP_076548250             953 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X18 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548250
VERSION     XP_076548250.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692135.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..953
                     /product="disks large 1 tumor suppressor protein isoform
                     X18"
                     /calculated_mol_wt=105722
     Region          35..92
                     /region_name="L27_1"
                     /note="pfam09058"
                     /db_xref="CDD:462665"
     Region          247..335
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(258..264,281,284,314,318,321..322)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          342..431
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(351..357,374,377,412,416,419..420)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          502..592
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(514..520,531,534,564,568,571..572)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          623..683
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(625,647..648)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(628,630,633,642,660..661,680,682..683)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          761..939
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..953
                     /gene="LOC117607241"
                     /coded_by="XM_076692135.1:329..3190"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mpvrkqggef dqffgcdntd iitdfaprpa rklaeahral elledyhakl trpqdkqlrl
       61 aiervirifk srlfqalldi qefyeltlld esksvqqkta etiriadkwe asdgpitptf
      121 aqndggpafp vnqtlsniyg rrsaspdvad hvdetqkpry tgggveeqlp pppsppqlke
      181 agqldgvprp nsvdrilhpd gsqhiltshe alnkshdswq ghdgdqahtp llaadtrhvn
      241 gdddweyeei vlerggaglg fsiaggtdnp hfgndtaiyi tklipggaas adgrlrvndt
      301 ilqvndvsvv dvphaaavda lkragntvkl yvrrrrhtql ieielikgnk glgfsiaggi
      361 gnqhipgdng iyvtkimegg aaqvdgrlvv gdklvavrna lqgdknlenv theeavatlk
      421 atqdrvvllv akpetgivpp ppppmasdns lspqprkqng svsalennst lpysqesrha
      481 sslalhgaat pravsqedvs revrtvvlnk gssglgfniv ggedgegifi sfilaggpad
      541 lsgelrrgdq ilsvnginlr tatheeaaaa lkgtgqtvti vvqykpedyn rfeakihdlk
      601 qqisqqnmmt gtlmrtsqkk slyvralfdy dpnkddglps rglafrygei lhvtnasdde
      661 wwqarrvtpq gdeeglgiip srrrwerkqr ardrsvkfqg hmpvildkqs tldrkkknfs
      721 fsrkfpfmks kddksedgsd qerseenvls yeavqqltiq ytrpviilgp lkdrinddli
      781 sefpdkfgsc vphttrsrre yevdgrdyhf vasreqmerd iqnhlfieag qyndnlygts
      841 vasvrevaek gkhcildvsg naikrlqvaq lypiaifikp ksvesvmemn krmteeqakk
      901 tyeralkmeq efgeyftavv qgdtpeeiyv kvkeviaeqs gpniwvpcrd qql
//
LOCUS       XP_034186600             923 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X19 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186600
VERSION     XP_034186600.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330709.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..923
                     /product="disks large 1 tumor suppressor protein isoform
                     X19"
                     /calculated_mol_wt=102748
     Region          152..240
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(163..169,186,189,219,223,226..227)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          247..336
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(256..262,279,282,317,321,324..325)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          407..497
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(419..425,436,439,469,473,476..477)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          528..588
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(530,552..553)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(533,535,538,547,565..566,585,587..588)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          731..909
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..923
                     /gene="LOC117607241"
                     /coded_by="XM_034330709.2:68..2839"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 midwvsivrh srrrfsdyvy gvpikvrkrr tkrscqfleh agnrnvttkr ssnicgccwn
       61 hkprytgggv eeqlppppsp pqlkeagqld gvprpnsvdr ilhpdgsqhi ltshealnks
      121 hdswqghdgd qahtpllaad trhvngdddw eyeeivlerg gaglgfsiag gtdnphfgnd
      181 taiyitklip ggaasadgrl rvndtilqvn dvsvvdvpha aavdalkrag ntvklyvrrr
      241 rhtqlieiel ikgnkglgfs iaggignqhi pgdngiyvtk imeggaaqvd grlvvgdklv
      301 avrnalqgdk nlenvtheea vatlkatqdr vvllvakpet givppppppm asdnslspqp
      361 rkqngsvsal ennstlpysq esrhasslal hgaatpravs qedvsrevrt vvlnkgssgl
      421 gfnivggedg egifisfila ggpadlsgel rrgdqilsvn ginlrtathe eaaaalkgtg
      481 qtvtivvqyk pedynrfeak ihdlkqqisq qnmmtgtlmr tsqkkslyvr alfdydpnkd
      541 dglpsrglaf rygeilhvtn asddewwqar rvtpqgdeeg lgiipsrrrw erkqrardrs
      601 vkfqghmpvi ldkidglqqs tldrkkknfs fsrkfpfmks kddksedgsd qerspttpen
      661 endptpfmlc ytqddtnteg sreilyrvel pyieeltlva lekeddennd elsseenvls
      721 yeavqqltiq ytrpviilgp lkdrinddli sefpdkfgsc vphttrsrre yevdgrdyhf
      781 vasreqmerd iqnhlfieag qyndnlygts vasvrevaek gkhcildvsg naikrlqvaq
      841 lypiaifikp ksvesvmemn krmteeqakk tyeralkmeq efgeyftavv qgdtpeeiyv
      901 kvkeviaeqs gpniwvpcrd qql
//
LOCUS       XP_076548251             853 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X20 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548251
VERSION     XP_076548251.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692136.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..853
                     /product="disks large 1 tumor suppressor protein isoform
                     X20"
                     /calculated_mol_wt=94632
     Region          82..170
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(93..99,116,119,149,153,156..157)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          177..266
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(186..192,209,212,247,251,254..255)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          337..427
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(349..355,366,369,399,403,406..407)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          458..518
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(460,482..483)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(463,465,468,477,495..496,515,517..518)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          661..839
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..853
                     /gene="LOC117607241"
                     /coded_by="XM_076692136.1:16..2577"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mppttptdsa mcslcprskk aqhpkklvrf dsivdtfsis dsnaiinqrs hdswqghdgd
       61 qahtpllaad trhvngdddw eyeeivlerg gaglgfsiag gtdnphfgnd taiyitklip
      121 ggaasadgrl rvndtilqvn dvsvvdvpha aavdalkrag ntvklyvrrr rhtqlieiel
      181 ikgnkglgfs iaggignqhi pgdngiyvtk imeggaaqvd grlvvgdklv avrnalqgdk
      241 nlenvtheea vatlkatqdr vvllvakpet givppppppm asdnslspqp rkqngsvsal
      301 ennstlpysq esrhasslal hgaatpravs qedvsrevrt vvlnkgssgl gfnivggedg
      361 egifisfila ggpadlsgel rrgdqilsvn ginlrtathe eaaaalkgtg qtvtivvqyk
      421 pedynrfeak ihdlkqqisq qnmmtgtlmr tsqkkslyvr alfdydpnkd dglpsrglaf
      481 rygeilhvtn asddewwqar rvtpqgdeeg lgiipsrrrw erkqrardrs vkfqghmpvi
      541 ldkidglqqs tldrkkknfs fsrkfpfmks kddksedgsd qerspttpen endptpfmlc
      601 ytqddtnteg sreilyrvel pyieeltlva lekeddennd elsseenvls yeavqqltiq
      661 ytrpviilgp lkdrinddli sefpdkfgsc vphttrsrre yevdgrdyhf vasreqmerd
      721 iqnhlfieag qyndnlygts vasvrevaek gkhcildvsg naikrlqvaq lypiaifikp
      781 ksvesvmemn krmteeqakk tyeralkmeq efgeyftavv qgdtpeeiyv kvkeviaeqs
      841 gpniwvpcrd qql
//
LOCUS       XP_076548252             841 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X21 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548252
VERSION     XP_076548252.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692137.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..841
                     /product="disks large 1 tumor suppressor protein isoform
                     X21"
                     /calculated_mol_wt=93348
     Region          70..158
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(81..87,104,107,137,141,144..145)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          165..254
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(174..180,197,200,235,239,242..243)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          325..415
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(337..343,354,357,387,391,394..395)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          446..506
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(448,470..471)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(451,453,456,465,483..484,503,505..506)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          649..827
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..841
                     /gene="LOC117607241"
                     /coded_by="XM_076692137.1:16..2541"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mppttptdsa mcslcprskk aqhpkklvrf dsivdtfsis dsnaiinqrs hdswqghdgd
       61 qvngdddwey eeivlergga glgfsiaggt dnphfgndta iyitklipgg aasadgrlrv
      121 ndtilqvndv svvdvphaaa vdalkragnt vklyvrrrrh tqlieielik gnkglgfsia
      181 ggignqhipg dngiyvtkim eggaaqvdgr lvvgdklvav rnalqgdknl envtheeava
      241 tlkatqdrvv llvakpetgi vppppppmas dnslspqprk qngsvsalen nstlpysqes
      301 rhasslalhg aatpravsqe dvsrevrtvv lnkgssglgf nivggedgeg ifisfilagg
      361 padlsgelrr gdqilsvngi nlrtatheea aaalkgtgqt vtivvqykpe dynrfeakih
      421 dlkqqisqqn mmtgtlmrts qkkslyvral fdydpnkddg lpsrglafry geilhvtnas
      481 ddewwqarrv tpqgdeeglg iipsrrrwer kqrardrsvk fqghmpvild kidglqqstl
      541 drkkknfsfs rkfpfmkskd dksedgsdqe rspttpenen dptpfmlcyt qddtntegsr
      601 eilyrvelpy ieeltlvale keddenndel sseenvlsye avqqltiqyt rpviilgplk
      661 drinddlise fpdkfgscvp httrsrreye vdgrdyhfva sreqmerdiq nhlfieagqy
      721 ndnlygtsva svrevaekgk hcildvsgna ikrlqvaqly piaifikpks vesvmemnkr
      781 mteeqakkty eralkmeqef geyftavvqg dtpeeiyvkv keviaeqsgp niwvpcrdqq
      841 l
//
LOCUS       XP_076548253             837 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X22 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548253
VERSION     XP_076548253.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692138.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..837
                     /product="disks large 1 tumor suppressor protein isoform
                     X22"
                     /calculated_mol_wt=93275
     Region          66..154
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(77..83,100,103,133,137,140..141)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          161..250
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(170..176,193,196,231,235,238..239)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          321..411
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(333..339,350,353,383,387,390..391)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          442..502
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(444,466..467)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(447,449,452,461,479..480,499,501..502)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          645..823
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..837
                     /gene="LOC117607241"
                     /coded_by="XM_076692138.1:10..2523"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mgmgfndkkl rrlrrlrlfg rakvyltvrw pqlshdswqg hdgdqahtpl laadtrhvng
       61 dddweyeeiv lerggaglgf siaggtdnph fgndtaiyit klipggaasa dgrlrvndti
      121 lqvndvsvvd vphaaavdal kragntvkly vrrrrhtqli eielikgnkg lgfsiaggig
      181 nqhipgdngi yvtkimegga aqvdgrlvvg dklvavrnal qgdknlenvt heeavatlka
      241 tqdrvvllva kpetgivppp pppmasdnsl spqprkqngs vsalennstl pysqesrhas
      301 slalhgaatp ravsqedvsr evrtvvlnkg ssglgfnivg gedgegifis filaggpadl
      361 sgelrrgdqi lsvnginlrt atheeaaaal kgtgqtvtiv vqykpedynr feakihdlkq
      421 qisqqnmmtg tlmrtsqkks lyvralfdyd pnkddglpsr glafrygeil hvtnasddew
      481 wqarrvtpqg deeglgiips rrrwerkqra rdrsvkfqgh mpvildkidg lqqstldrkk
      541 knfsfsrkfp fmkskddkse dgsdqerspt tpenendptp fmlcytqddt ntegsreily
      601 rvelpyieel tlvalekedd enndelssee nvlsyeavqq ltiqytrpvi ilgplkdrin
      661 ddlisefpdk fgscvphttr srreyevdgr dyhfvasreq merdiqnhlf ieagqyndnl
      721 ygtsvasvre vaekgkhcil dvsgnaikrl qvaqlypiai fikpksvesv memnkrmtee
      781 qakktyeral kmeqefgeyf tavvqgdtpe eiyvkvkevi aeqsgpniwv pcrdqql
//
LOCUS       XP_076548254             825 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X23 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548254
VERSION     XP_076548254.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692139.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..825
                     /product="disks large 1 tumor suppressor protein isoform
                     X23"
                     /calculated_mol_wt=91991
     Region          54..142
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(65..71,88,91,121,125,128..129)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          149..238
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(158..164,181,184,219,223,226..227)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          309..399
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(321..327,338,341,371,375,378..379)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          430..490
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(432,454..455)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(435,437,440,449,467..468,487,489..490)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          633..811
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..825
                     /gene="LOC117607241"
                     /coded_by="XM_076692139.1:10..2487"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mgmgfndkkl rrlrrlrlfg rakvyltvrw pqlshdswqg hdgdqvngdd dweyeeivle
       61 rggaglgfsi aggtdnphfg ndtaiyitkl ipggaasadg rlrvndtilq vndvsvvdvp
      121 haaavdalkr agntvklyvr rrrhtqliei elikgnkglg fsiaggignq hipgdngiyv
      181 tkimeggaaq vdgrlvvgdk lvavrnalqg dknlenvthe eavatlkatq drvvllvakp
      241 etgivppppp pmasdnslsp qprkqngsvs alennstlpy sqesrhassl alhgaatpra
      301 vsqedvsrev rtvvlnkgss glgfnivgge dgegifisfi laggpadlsg elrrgdqils
      361 vnginlrtat heeaaaalkg tgqtvtivvq ykpedynrfe akihdlkqqi sqqnmmtgtl
      421 mrtsqkksly vralfdydpn kddglpsrgl afrygeilhv tnasddewwq arrvtpqgde
      481 eglgiipsrr rwerkqrard rsvkfqghmp vildkidglq qstldrkkkn fsfsrkfpfm
      541 kskddksedg sdqerspttp enendptpfm lcytqddtnt egsreilyrv elpyieeltl
      601 valekedden ndelsseenv lsyeavqqlt iqytrpviil gplkdrindd lisefpdkfg
      661 scvphttrsr reyevdgrdy hfvasreqme rdiqnhlfie agqyndnlyg tsvasvreva
      721 ekgkhcildv sgnaikrlqv aqlypiaifi kpksvesvme mnkrmteeqa kktyeralkm
      781 eqefgeyfta vvqgdtpeei yvkvkeviae qsgpniwvpc rdqql
//
LOCUS       XP_076548255             817 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X24 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548255
VERSION     XP_076548255.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692140.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..817
                     /product="disks large 1 tumor suppressor protein isoform
                     X24"
                     /calculated_mol_wt=90763
     Region          46..134
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(57..63,80,83,113,117,120..121)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          141..230
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(150..156,173,176,211,215,218..219)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          301..391
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(313..319,330,333,363,367,370..371)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          422..482
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(424,446..447)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(427,429,432,441,459..460,479,481..482)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          625..803
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..817
                     /gene="LOC117607241"
                     /coded_by="XM_076692140.1:1200..3653"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mttrrkkksq dgflkkvssl fnldsahtpl laadtrhvng dddweyeeiv lerggaglgf
       61 siaggtdnph fgndtaiyit klipggaasa dgrlrvndti lqvndvsvvd vphaaavdal
      121 kragntvkly vrrrrhtqli eielikgnkg lgfsiaggig nqhipgdngi yvtkimegga
      181 aqvdgrlvvg dklvavrnal qgdknlenvt heeavatlka tqdrvvllva kpetgivppp
      241 pppmasdnsl spqprkqngs vsalennstl pysqesrhas slalhgaatp ravsqedvsr
      301 evrtvvlnkg ssglgfnivg gedgegifis filaggpadl sgelrrgdqi lsvnginlrt
      361 atheeaaaal kgtgqtvtiv vqykpedynr feakihdlkq qisqqnmmtg tlmrtsqkks
      421 lyvralfdyd pnkddglpsr glafrygeil hvtnasddew wqarrvtpqg deeglgiips
      481 rrrwerkqra rdrsvkfqgh mpvildkidg lqqstldrkk knfsfsrkfp fmkskddkse
      541 dgsdqerspt tpenendptp fmlcytqddt ntegsreily rvelpyieel tlvalekedd
      601 enndelssee nvlsyeavqq ltiqytrpvi ilgplkdrin ddlisefpdk fgscvphttr
      661 srreyevdgr dyhfvasreq merdiqnhlf ieagqyndnl ygtsvasvre vaekgkhcil
      721 dvsgnaikrl qvaqlypiai fikpksvesv memnkrmtee qakktyeral kmeqefgeyf
      781 tavvqgdtpe eiyvkvkevi aeqsgpniwv pcrdqql
//
LOCUS       XP_076548256             817 aa            linear   INV 03-SEP-2025
DEFINITION  disks large 1 tumor suppressor protein isoform X24 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548256
VERSION     XP_076548256.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692141.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..817
                     /product="disks large 1 tumor suppressor protein isoform
                     X24"
                     /calculated_mol_wt=90763
     Region          46..134
                     /region_name="PDZ1_Dlg1-2-4-like"
                     /note="PDZ domain 1 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06723"
                     /db_xref="CDD:467206"
     Site            order(57..63,80,83,113,117,120..121)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467206"
     Region          141..230
                     /region_name="PDZ2_Dlg1-2-4-like"
                     /note="PDZ domain 2 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06724"
                     /db_xref="CDD:467207"
     Site            order(150..156,173,176,211,215,218..219)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467207"
     Region          301..391
                     /region_name="PDZ3_Dlg1-2-4-like"
                     /note="PDZ domain 3 of human discs large homolog 1 (Dlg1),
                     Dlg2, and Dlg4, Drosophila disc large (Dlg), and related
                     domains; cd06795"
                     /db_xref="CDD:467257"
     Site            order(313..319,330,333,363,367,370..371)
                     /site_type="other"
                     /note="peptide binding site [polypeptide binding]"
                     /db_xref="CDD:467257"
     Region          422..482
                     /region_name="SH3_DLG-like"
                     /note="Src Homology 3 domain of Disks large homolog
                     proteins; cd11861"
                     /db_xref="CDD:212795"
     Site            order(424,446..447)
                     /site_type="other"
                     /note="GuK domain interface [polypeptide binding]"
                     /db_xref="CDD:212795"
     Site            order(427,429,432,441,459..460,479,481..482)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212795"
     Region          625..803
                     /region_name="Guanylate_kin"
                     /note="Guanylate kinase; pfam00625"
                     /db_xref="CDD:395500"
     CDS             1..817
                     /gene="LOC117607241"
                     /coded_by="XM_076692141.1:1103..3556"
                     /db_xref="GeneID:117607241"
ORIGIN      
        1 mttrrkkksq dgflkkvssl fnldsahtpl laadtrhvng dddweyeeiv lerggaglgf
       61 siaggtdnph fgndtaiyit klipggaasa dgrlrvndti lqvndvsvvd vphaaavdal
      121 kragntvkly vrrrrhtqli eielikgnkg lgfsiaggig nqhipgdngi yvtkimegga
      181 aqvdgrlvvg dklvavrnal qgdknlenvt heeavatlka tqdrvvllva kpetgivppp
      241 pppmasdnsl spqprkqngs vsalennstl pysqesrhas slalhgaatp ravsqedvsr
      301 evrtvvlnkg ssglgfnivg gedgegifis filaggpadl sgelrrgdqi lsvnginlrt
      361 atheeaaaal kgtgqtvtiv vqykpedynr feakihdlkq qisqqnmmtg tlmrtsqkks
      421 lyvralfdyd pnkddglpsr glafrygeil hvtnasddew wqarrvtpqg deeglgiips
      481 rrrwerkqra rdrsvkfqgh mpvildkidg lqqstldrkk knfsfsrkfp fmkskddkse
      541 dgsdqerspt tpenendptp fmlcytqddt ntegsreily rvelpyieel tlvalekedd
      601 enndelssee nvlsyeavqq ltiqytrpvi ilgplkdrin ddlisefpdk fgscvphttr
      661 srreyevdgr dyhfvasreq merdiqnhlf ieagqyndnl ygtsvasvre vaekgkhcil
      721 dvsgnaikrl qvaqlypiai fikpksvesv memnkrmtee qakktyeral kmeqefgeyf
      781 tavvqgdtpe eiyvkvkevi aeqsgpniwv pcrdqql
//
LOCUS       XP_034186493             882 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186493
VERSION     XP_034186493.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330602.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186493.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..882
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X1"
                     /calculated_mol_wt=98443
     Region          68..628
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          771..877
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..882
                     /gene="AP-1gamma"
                     /coded_by="XM_034330602.2:110..2758"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mwpyyecedw svlppelnrr fnpafnmasi kqvfneaver vstvrmpapt rlrdlirqir
       61 aartaaeert vvnkecayir stfreedsvw rcrniaklly ihmlgypahf gqleclklia
      121 sprftdkrig ylgamlllde rqdvhllitn clkndlnsst qfviglalct lgaiaspema
      181 rdlaseverl mkspnayirk kaalcafrii rrvpelmemf lpatrslite knhgvlitgv
      241 tlitemcens vdtlnhfkke cghreivpnl vrilknlila gyspehdvsg vsdpflqvki
      301 lrllrilgrn didaseamnd ilaqvatnte tsknvgntil yetvlsimdi ksesglrvla
      361 vnilgrflln ndkniryval ntllktvyvd tsavqrhrst ileclkdpdv sirrramels
      421 falvnsnnir nmmkelllfl eradpefkaq cssnivmsae rfapnkrwhl etlfkvlvaa
      481 gnyvrddvva ctiqlisetq aqqsyavsal wkalekdtad kqplaqvatw cigeygdlll
      541 yappsedvda pvnltedevi dvyqrllwsp qntvvtkqyt llsltklstr fqkgnekirq
      601 iidtfgsnlh ielqqrgief sqlfrkydhl rsallermpp metarpqang iigmangepe
      661 peeekstale astppsdssa lldllgttdl gmtpsvsskn ppanstavvn nndlldllgs
      721 ldlsaptpts tlpqtqsptq ifnpanttnf lvdgllnssq siqndtpvmi vldkaglkit
      781 frlerpsdvn dllvinmsaq nsgsailtdf lfqaavpktf qlqmlspsst vippsgqvtq
      841 vlkvaninkv plrmrlrisy tgpagpvleq tevnnfppit sq
//
LOCUS       XP_034186494             879 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186494
VERSION     XP_034186494.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330603.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186494.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..879
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X2"
                     /calculated_mol_wt=98156
     Region          65..625
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          768..874
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..879
                     /gene="AP-1gamma"
                     /coded_by="XM_034330603.2:109..2748"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mwpyyecedw svlppelnrr fnpafnmasi kqvfneaver vrmpaptrlr dlirqiraar
       61 taaeertvvn kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr
      121 ftdkrigylg amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl
      181 aseverlmks pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli
      241 temcensvdt lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl
      301 lrilgrndid aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni
      361 lgrfllnndk niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal
      421 vnsnnirnmm kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny
      481 vrddvvacti qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap
      541 psedvdapvn ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid
      601 tfgsnlhiel qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee
      661 ekstaleast ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl
      721 saptptstlp qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl
      781 erpsdvndll vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk
      841 vaninkvplr mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_034186495             876 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186495
VERSION     XP_034186495.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330604.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186495.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..876
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X3"
                     /calculated_mol_wt=97731
     Region          68..622
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          765..871
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..876
                     /gene="AP-1gamma"
                     /coded_by="XM_034330604.2:108..2738"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mwpyyecedw svlppelnrr fnpafnmasi kqvfneaver vstvrmpapt rlrdlirqir
       61 aartaaeert vvnkecayir stfreedsvw rcrniaklly ihmlgypahf gqleclklia
      121 sprftdkrig ylgamlllde rqdvhllitn clkndlnsst qfviglalct lgaiaspema
      181 rdlaseverl mkspnayirk kaalcafrii rrvpelmemf lpatrslite knhgvlitgv
      241 tlitemcens vdtlnhfkki vpnlvrilkn lilagyspeh dvsgvsdpfl qvkilrllri
      301 lgrndidase amndilaqva tntetsknvg ntilyetvls imdiksesgl rvlavnilgr
      361 fllnndknir yvalntllkt vyvdtsavqr hrstileclk dpdvsirrra melsfalvns
      421 nnirnmmkel llfleradpe fkaqcssniv msaerfapnk rwhletlfkv lvaagnyvrd
      481 dvvactiqli setqaqqsya vsalwkalek dtadkqplaq vatwcigeyg dlllyappse
      541 dvdapvnlte devidvyqrl lwspqntvvt kqytllsltk lstrfqkgne kirqiidtfg
      601 snlhielqqr giefsqlfrk ydhlrsalle rmppmetarp qangiigman gepepeeeks
      661 taleastpps dssalldllg ttdlgmtpsv ssknppanst avvnnndlld llgsldlsap
      721 tptstlpqtq sptqifnpan ttnflvdgll nssqsiqndt pvmivldkag lkitfrlerp
      781 sdvndllvin msaqnsgsai ltdflfqaav pktfqlqmls psstvippsg qvtqvlkvan
      841 inkvplrmrl risytgpagp vleqtevnnf ppitsq
//
LOCUS       XP_034186496             873 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186496
VERSION     XP_034186496.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330605.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186496.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..873
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X4"
                     /calculated_mol_wt=97444
     Region          65..619
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          762..868
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..873
                     /gene="AP-1gamma"
                     /coded_by="XM_034330605.2:108..2729"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mwpyyecedw svlppelnrr fnpafnmasi kqvfneaver vrmpaptrlr dlirqiraar
       61 taaeertvvn kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr
      121 ftdkrigylg amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl
      181 aseverlmks pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli
      241 temcensvdt lnhfkkivpn lvrilknlil agyspehdvs gvsdpflqvk ilrllrilgr
      301 ndidaseamn dilaqvatnt etsknvgnti lyetvlsimd iksesglrvl avnilgrfll
      361 nndkniryva lntllktvyv dtsavqrhrs tileclkdpd vsirrramel sfalvnsnni
      421 rnmmkelllf leradpefka qcssnivmsa erfapnkrwh letlfkvlva agnyvrddvv
      481 actiqliset qaqqsyavsa lwkalekdta dkqplaqvat wcigeygdll lyappsedvd
      541 apvnltedev idvyqrllws pqntvvtkqy tllsltklst rfqkgnekir qiidtfgsnl
      601 hielqqrgie fsqlfrkydh lrsallermp pmetarpqan giigmangep epeeekstal
      661 eastppsdss alldllgttd lgmtpsvssk nppanstavv nnndlldllg sldlsaptpt
      721 stlpqtqspt qifnpanttn flvdgllnss qsiqndtpvm ivldkaglki tfrlerpsdv
      781 ndllvinmsa qnsgsailtd flfqaavpkt fqlqmlspss tvippsgqvt qvlkvanink
      841 vplrmrlris ytgpagpvle qtevnnfppi tsq
//
LOCUS       XP_076548258             869 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548258
VERSION     XP_076548258.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692143.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=96604
     Region          55..615
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          758..864
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..869
                     /gene="AP-1gamma"
                     /coded_by="XM_076692143.1:953..3562"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervst vrmpaptrlr dlirqiraar taaeertvvn
       61 kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr ftdkrigylg
      121 amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl aseverlmks
      181 pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli temcensvdt
      241 lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl lrilgrndid
      301 aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni lgrfllnndk
      361 niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal vnsnnirnmm
      421 kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny vrddvvacti
      481 qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap psedvdapvn
      541 ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid tfgsnlhiel
      601 qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee ekstaleast
      661 ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl saptptstlp
      721 qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl erpsdvndll
      781 vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk vaninkvplr
      841 mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_034186497             869 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186497
VERSION     XP_034186497.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330606.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186497.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=96604
     Region          55..615
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          758..864
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..869
                     /gene="AP-1gamma"
                     /coded_by="XM_034330606.2:780..3389"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervst vrmpaptrlr dlirqiraar taaeertvvn
       61 kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr ftdkrigylg
      121 amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl aseverlmks
      181 pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli temcensvdt
      241 lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl lrilgrndid
      301 aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni lgrfllnndk
      361 niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal vnsnnirnmm
      421 kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny vrddvvacti
      481 qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap psedvdapvn
      541 ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid tfgsnlhiel
      601 qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee ekstaleast
      661 ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl saptptstlp
      721 qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl erpsdvndll
      781 vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk vaninkvplr
      841 mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_076548259             869 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548259
VERSION     XP_076548259.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692144.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=96604
     Region          55..615
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          758..864
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..869
                     /gene="AP-1gamma"
                     /coded_by="XM_076692144.1:665..3274"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervst vrmpaptrlr dlirqiraar taaeertvvn
       61 kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr ftdkrigylg
      121 amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl aseverlmks
      181 pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli temcensvdt
      241 lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl lrilgrndid
      301 aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni lgrfllnndk
      361 niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal vnsnnirnmm
      421 kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny vrddvvacti
      481 qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap psedvdapvn
      541 ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid tfgsnlhiel
      601 qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee ekstaleast
      661 ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl saptptstlp
      721 qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl erpsdvndll
      781 vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk vaninkvplr
      841 mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_034186498             869 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186498
VERSION     XP_034186498.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330607.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186498.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=96604
     Region          55..615
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          758..864
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..869
                     /gene="AP-1gamma"
                     /coded_by="XM_034330607.2:874..3483"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervst vrmpaptrlr dlirqiraar taaeertvvn
       61 kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr ftdkrigylg
      121 amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl aseverlmks
      181 pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli temcensvdt
      241 lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl lrilgrndid
      301 aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni lgrfllnndk
      361 niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal vnsnnirnmm
      421 kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny vrddvvacti
      481 qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap psedvdapvn
      541 ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid tfgsnlhiel
      601 qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee ekstaleast
      661 ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl saptptstlp
      721 qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl erpsdvndll
      781 vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk vaninkvplr
      841 mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_034186499             869 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186499
VERSION     XP_034186499.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330608.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186499.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=96604
     Region          55..615
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          758..864
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..869
                     /gene="AP-1gamma"
                     /coded_by="XM_034330608.2:584..3193"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervst vrmpaptrlr dlirqiraar taaeertvvn
       61 kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr ftdkrigylg
      121 amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl aseverlmks
      181 pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli temcensvdt
      241 lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl lrilgrndid
      301 aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni lgrfllnndk
      361 niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal vnsnnirnmm
      421 kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny vrddvvacti
      481 qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap psedvdapvn
      541 ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid tfgsnlhiel
      601 qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee ekstaleast
      661 ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl saptptstlp
      721 qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl erpsdvndll
      781 vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk vaninkvplr
      841 mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_034186500             869 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186500
VERSION     XP_034186500.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330609.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186500.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=96604
     Region          55..615
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          758..864
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..869
                     /gene="AP-1gamma"
                     /coded_by="XM_034330609.2:469..3078"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervst vrmpaptrlr dlirqiraar taaeertvvn
       61 kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr ftdkrigylg
      121 amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl aseverlmks
      181 pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli temcensvdt
      241 lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl lrilgrndid
      301 aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni lgrfllnndk
      361 niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal vnsnnirnmm
      421 kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny vrddvvacti
      481 qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap psedvdapvn
      541 ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid tfgsnlhiel
      601 qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee ekstaleast
      661 ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl saptptstlp
      721 qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl erpsdvndll
      781 vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk vaninkvplr
      841 mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_034186501             869 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186501
VERSION     XP_034186501.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330610.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186501.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..869
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X5"
                     /calculated_mol_wt=96604
     Region          55..615
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          758..864
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..869
                     /gene="AP-1gamma"
                     /coded_by="XM_034330610.2:586..3195"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervst vrmpaptrlr dlirqiraar taaeertvvn
       61 kecayirstf reedsvwrcr niakllyihm lgypahfgql eclkliaspr ftdkrigylg
      121 amlllderqd vhllitnclk ndlnsstqfv iglalctlga iaspemardl aseverlmks
      181 pnayirkkaa lcafriirrv pelmemflpa trsliteknh gvlitgvtli temcensvdt
      241 lnhfkkecgh reivpnlvri lknlilagys pehdvsgvsd pflqvkilrl lrilgrndid
      301 aseamndila qvatntetsk nvgntilyet vlsimdikse sglrvlavni lgrfllnndk
      361 niryvalntl lktvyvdtsa vqrhrstile clkdpdvsir rramelsfal vnsnnirnmm
      421 kelllflera dpefkaqcss nivmsaerfa pnkrwhletl fkvlvaagny vrddvvacti
      481 qlisetqaqq syavsalwka lekdtadkqp laqvatwcig eygdlllyap psedvdapvn
      541 ltedevidvy qrllwspqnt vvtkqytlls ltklstrfqk gnekirqiid tfgsnlhiel
      601 qqrgiefsql frkydhlrsa llermppmet arpqangiig mangepepee ekstaleast
      661 ppsdssalld llgttdlgmt psvssknppa nstavvnnnd lldllgsldl saptptstlp
      721 qtqsptqifn panttnflvd gllnssqsiq ndtpvmivld kaglkitfrl erpsdvndll
      781 vinmsaqnsg sailtdflfq aavpktfqlq mlspsstvip psgqvtqvlk vaninkvplr
      841 mrlrisytgp agpvleqtev nnfppitsq
//
LOCUS       XP_034186502             866 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186502
VERSION     XP_034186502.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330611.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186502.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..866
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X6"
                     /calculated_mol_wt=96316
     Region          52..612
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          755..861
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..866
                     /gene="AP-1gamma"
                     /coded_by="XM_034330611.2:949..3549"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervrm paptrlrdli rqiraartaa eertvvnkec
       61 ayirstfree dsvwrcrnia kllyihmlgy pahfgqlecl kliasprftd krigylgaml
      121 llderqdvhl litnclkndl nsstqfvigl alctlgaias pemardlase verlmkspna
      181 yirkkaalca friirrvpel memflpatrs liteknhgvl itgvtlitem censvdtlnh
      241 fkkecghrei vpnlvrilkn lilagyspeh dvsgvsdpfl qvkilrllri lgrndidase
      301 amndilaqva tntetsknvg ntilyetvls imdiksesgl rvlavnilgr fllnndknir
      361 yvalntllkt vyvdtsavqr hrstileclk dpdvsirrra melsfalvns nnirnmmkel
      421 llfleradpe fkaqcssniv msaerfapnk rwhletlfkv lvaagnyvrd dvvactiqli
      481 setqaqqsya vsalwkalek dtadkqplaq vatwcigeyg dlllyappse dvdapvnlte
      541 devidvyqrl lwspqntvvt kqytllsltk lstrfqkgne kirqiidtfg snlhielqqr
      601 giefsqlfrk ydhlrsalle rmppmetarp qangiigman gepepeeeks taleastpps
      661 dssalldllg ttdlgmtpsv ssknppanst avvnnndlld llgsldlsap tptstlpqtq
      721 sptqifnpan ttnflvdgll nssqsiqndt pvmivldkag lkitfrlerp sdvndllvin
      781 msaqnsgsai ltdflfqaav pktfqlqmls psstvippsg qvtqvlkvan inkvplrmrl
      841 risytgpagp vleqtevnnf ppitsq
//
LOCUS       XP_034186504             860 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186504
VERSION     XP_034186504.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330613.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186504.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..860
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X7"
                     /calculated_mol_wt=95605
     Region          52..606
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          749..855
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..860
                     /gene="AP-1gamma"
                     /coded_by="XM_034330613.2:942..3524"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mnasehgfnp afnmasikqv fneavervrm paptrlrdli rqiraartaa eertvvnkec
       61 ayirstfree dsvwrcrnia kllyihmlgy pahfgqlecl kliasprftd krigylgaml
      121 llderqdvhl litnclkndl nsstqfvigl alctlgaias pemardlase verlmkspna
      181 yirkkaalca friirrvpel memflpatrs liteknhgvl itgvtlitem censvdtlnh
      241 fkkivpnlvr ilknlilagy spehdvsgvs dpflqvkilr llrilgrndi daseamndil
      301 aqvatntets knvgntilye tvlsimdiks esglrvlavn ilgrfllnnd kniryvalnt
      361 llktvyvdts avqrhrstil eclkdpdvsi rrramelsfa lvnsnnirnm mkelllfler
      421 adpefkaqcs snivmsaerf apnkrwhlet lfkvlvaagn yvrddvvact iqlisetqaq
      481 qsyavsalwk alekdtadkq plaqvatwci geygdlllya ppsedvdapv nltedevidv
      541 yqrllwspqn tvvtkqytll sltklstrfq kgnekirqii dtfgsnlhie lqqrgiefsq
      601 lfrkydhlrs allermppme tarpqangii gmangepepe eekstaleas tppsdssall
      661 dllgttdlgm tpsvssknpp anstavvnnn dlldllgsld lsaptptstl pqtqsptqif
      721 npanttnflv dgllnssqsi qndtpvmivl dkaglkitfr lerpsdvndl lvinmsaqns
      781 gsailtdflf qaavpktfql qmlspsstvi ppsgqvtqvl kvaninkvpl rmrlrisytg
      841 pagpvleqte vnnfppitsq
//
LOCUS       XP_034186505             844 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186505
VERSION     XP_034186505.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330614.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186505.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X8"
                     /calculated_mol_wt=93883
     Region          30..590
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          733..839
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..844
                     /gene="AP-1gamma"
                     /coded_by="XM_034330614.2:954..3488"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mlqntvrmpa ptrlrdlirq iraartaaee rtvvnkecay irstfreeds vwrcrniakl
       61 lyihmlgypa hfgqleclkl iasprftdkr igylgamlll derqdvhlli tnclkndlns
      121 stqfviglal ctlgaiaspe mardlaseve rlmkspnayi rkkaalcafr iirrvpelme
      181 mflpatrsli teknhgvlit gvtlitemce nsvdtlnhfk kecghreivp nlvrilknli
      241 lagyspehdv sgvsdpflqv kilrllrilg rndidaseam ndilaqvatn tetsknvgnt
      301 ilyetvlsim diksesglrv lavnilgrfl lnndkniryv alntllktvy vdtsavqrhr
      361 stileclkdp dvsirrrame lsfalvnsnn irnmmkelll fleradpefk aqcssnivms
      421 aerfapnkrw hletlfkvlv aagnyvrddv vactiqlise tqaqqsyavs alwkalekdt
      481 adkqplaqva twcigeygdl llyappsedv dapvnltede vidvyqrllw spqntvvtkq
      541 ytllsltkls trfqkgneki rqiidtfgsn lhielqqrgi efsqlfrkyd hlrsallerm
      601 ppmetarpqa ngiigmange pepeeeksta leastppsds salldllgtt dlgmtpsvss
      661 knppanstav vnnndlldll gsldlsaptp tstlpqtqsp tqifnpantt nflvdgllns
      721 sqsiqndtpv mivldkaglk itfrlerpsd vndllvinms aqnsgsailt dflfqaavpk
      781 tfqlqmlsps stvippsgqv tqvlkvanin kvplrmrlri sytgpagpvl eqtevnnfpp
      841 itsq
//
LOCUS       XP_034186506             844 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186506
VERSION     XP_034186506.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330615.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186506.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X8"
                     /calculated_mol_wt=93883
     Region          30..590
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          733..839
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..844
                     /gene="AP-1gamma"
                     /coded_by="XM_034330615.2:781..3315"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mlqntvrmpa ptrlrdlirq iraartaaee rtvvnkecay irstfreeds vwrcrniakl
       61 lyihmlgypa hfgqleclkl iasprftdkr igylgamlll derqdvhlli tnclkndlns
      121 stqfviglal ctlgaiaspe mardlaseve rlmkspnayi rkkaalcafr iirrvpelme
      181 mflpatrsli teknhgvlit gvtlitemce nsvdtlnhfk kecghreivp nlvrilknli
      241 lagyspehdv sgvsdpflqv kilrllrilg rndidaseam ndilaqvatn tetsknvgnt
      301 ilyetvlsim diksesglrv lavnilgrfl lnndkniryv alntllktvy vdtsavqrhr
      361 stileclkdp dvsirrrame lsfalvnsnn irnmmkelll fleradpefk aqcssnivms
      421 aerfapnkrw hletlfkvlv aagnyvrddv vactiqlise tqaqqsyavs alwkalekdt
      481 adkqplaqva twcigeygdl llyappsedv dapvnltede vidvyqrllw spqntvvtkq
      541 ytllsltkls trfqkgneki rqiidtfgsn lhielqqrgi efsqlfrkyd hlrsallerm
      601 ppmetarpqa ngiigmange pepeeeksta leastppsds salldllgtt dlgmtpsvss
      661 knppanstav vnnndlldll gsldlsaptp tstlpqtqsp tqifnpantt nflvdgllns
      721 sqsiqndtpv mivldkaglk itfrlerpsd vndllvinms aqnsgsailt dflfqaavpk
      781 tfqlqmlsps stvippsgqv tqvlkvanin kvplrmrlri sytgpagpvl eqtevnnfpp
      841 itsq
//
LOCUS       XP_034186507             844 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186507
VERSION     XP_034186507.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330616.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186507.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X8"
                     /calculated_mol_wt=93883
     Region          30..590
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          733..839
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..844
                     /gene="AP-1gamma"
                     /coded_by="XM_034330616.2:666..3200"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mlqntvrmpa ptrlrdlirq iraartaaee rtvvnkecay irstfreeds vwrcrniakl
       61 lyihmlgypa hfgqleclkl iasprftdkr igylgamlll derqdvhlli tnclkndlns
      121 stqfviglal ctlgaiaspe mardlaseve rlmkspnayi rkkaalcafr iirrvpelme
      181 mflpatrsli teknhgvlit gvtlitemce nsvdtlnhfk kecghreivp nlvrilknli
      241 lagyspehdv sgvsdpflqv kilrllrilg rndidaseam ndilaqvatn tetsknvgnt
      301 ilyetvlsim diksesglrv lavnilgrfl lnndkniryv alntllktvy vdtsavqrhr
      361 stileclkdp dvsirrrame lsfalvnsnn irnmmkelll fleradpefk aqcssnivms
      421 aerfapnkrw hletlfkvlv aagnyvrddv vactiqlise tqaqqsyavs alwkalekdt
      481 adkqplaqva twcigeygdl llyappsedv dapvnltede vidvyqrllw spqntvvtkq
      541 ytllsltkls trfqkgneki rqiidtfgsn lhielqqrgi efsqlfrkyd hlrsallerm
      601 ppmetarpqa ngiigmange pepeeeksta leastppsds salldllgtt dlgmtpsvss
      661 knppanstav vnnndlldll gsldlsaptp tstlpqtqsp tqifnpantt nflvdgllns
      721 sqsiqndtpv mivldkaglk itfrlerpsd vndllvinms aqnsgsailt dflfqaavpk
      781 tfqlqmlsps stvippsgqv tqvlkvanin kvplrmrlri sytgpagpvl eqtevnnfpp
      841 itsq
//
LOCUS       XP_034186508             844 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186508
VERSION     XP_034186508.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330617.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186508.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X8"
                     /calculated_mol_wt=93883
     Region          30..590
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          733..839
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..844
                     /gene="AP-1gamma"
                     /coded_by="XM_034330617.2:585..3119"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mlqntvrmpa ptrlrdlirq iraartaaee rtvvnkecay irstfreeds vwrcrniakl
       61 lyihmlgypa hfgqleclkl iasprftdkr igylgamlll derqdvhlli tnclkndlns
      121 stqfviglal ctlgaiaspe mardlaseve rlmkspnayi rkkaalcafr iirrvpelme
      181 mflpatrsli teknhgvlit gvtlitemce nsvdtlnhfk kecghreivp nlvrilknli
      241 lagyspehdv sgvsdpflqv kilrllrilg rndidaseam ndilaqvatn tetsknvgnt
      301 ilyetvlsim diksesglrv lavnilgrfl lnndkniryv alntllktvy vdtsavqrhr
      361 stileclkdp dvsirrrame lsfalvnsnn irnmmkelll fleradpefk aqcssnivms
      421 aerfapnkrw hletlfkvlv aagnyvrddv vactiqlise tqaqqsyavs alwkalekdt
      481 adkqplaqva twcigeygdl llyappsedv dapvnltede vidvyqrllw spqntvvtkq
      541 ytllsltkls trfqkgneki rqiidtfgsn lhielqqrgi efsqlfrkyd hlrsallerm
      601 ppmetarpqa ngiigmange pepeeeksta leastppsds salldllgtt dlgmtpsvss
      661 knppanstav vnnndlldll gsldlsaptp tstlpqtqsp tqifnpantt nflvdgllns
      721 sqsiqndtpv mivldkaglk itfrlerpsd vndllvinms aqnsgsailt dflfqaavpk
      781 tfqlqmlsps stvippsgqv tqvlkvanin kvplrmrlri sytgpagpvl eqtevnnfpp
      841 itsq
//
LOCUS       XP_034186509             844 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186509
VERSION     XP_034186509.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330618.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186509.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..844
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X8"
                     /calculated_mol_wt=93883
     Region          30..590
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          733..839
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..844
                     /gene="AP-1gamma"
                     /coded_by="XM_034330618.2:471..3005"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mlqntvrmpa ptrlrdlirq iraartaaee rtvvnkecay irstfreeds vwrcrniakl
       61 lyihmlgypa hfgqleclkl iasprftdkr igylgamlll derqdvhlli tnclkndlns
      121 stqfviglal ctlgaiaspe mardlaseve rlmkspnayi rkkaalcafr iirrvpelme
      181 mflpatrsli teknhgvlit gvtlitemce nsvdtlnhfk kecghreivp nlvrilknli
      241 lagyspehdv sgvsdpflqv kilrllrilg rndidaseam ndilaqvatn tetsknvgnt
      301 ilyetvlsim diksesglrv lavnilgrfl lnndkniryv alntllktvy vdtsavqrhr
      361 stileclkdp dvsirrrame lsfalvnsnn irnmmkelll fleradpefk aqcssnivms
      421 aerfapnkrw hletlfkvlv aagnyvrddv vactiqlise tqaqqsyavs alwkalekdt
      481 adkqplaqva twcigeygdl llyappsedv dapvnltede vidvyqrllw spqntvvtkq
      541 ytllsltkls trfqkgneki rqiidtfgsn lhielqqrgi efsqlfrkyd hlrsallerm
      601 ppmetarpqa ngiigmange pepeeeksta leastppsds salldllgtt dlgmtpsvss
      661 knppanstav vnnndlldll gsldlsaptp tstlpqtqsp tqifnpantt nflvdgllns
      721 sqsiqndtpv mivldkaglk itfrlerpsd vndllvinms aqnsgsailt dflfqaavpk
      781 tfqlqmlsps stvippsgqv tqvlkvanin kvplrmrlri sytgpagpvl eqtevnnfpp
      841 itsq
//
LOCUS       XP_034186510             837 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186510
VERSION     XP_034186510.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330619.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186510.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..837
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X9"
                     /calculated_mol_wt=93040
     Region          23..583
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          726..832
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..837
                     /gene="AP-1gamma"
                     /coded_by="XM_034330619.2:692..3205"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mpaptrlrdl irqiraarta aeertvvnke cayirstfre edsvwrcrni akllyihmlg
       61 ypahfgqlec lkliasprft dkrigylgam lllderqdvh llitnclknd lnsstqfvig
      121 lalctlgaia spemardlas everlmkspn ayirkkaalc afriirrvpe lmemflpatr
      181 sliteknhgv litgvtlite mcensvdtln hfkkecghre ivpnlvrilk nlilagyspe
      241 hdvsgvsdpf lqvkilrllr ilgrndidas eamndilaqv atntetsknv gntilyetvl
      301 simdiksesg lrvlavnilg rfllnndkni ryvalntllk tvyvdtsavq rhrstilecl
      361 kdpdvsirrr amelsfalvn snnirnmmke lllfleradp efkaqcssni vmsaerfapn
      421 krwhletlfk vlvaagnyvr ddvvactiql isetqaqqsy avsalwkale kdtadkqpla
      481 qvatwcigey gdlllyapps edvdapvnlt edevidvyqr llwspqntvv tkqytllslt
      541 klstrfqkgn ekirqiidtf gsnlhielqq rgiefsqlfr kydhlrsall ermppmetar
      601 pqangiigma ngepepeeek staleastpp sdssalldll gttdlgmtps vssknppans
      661 tavvnnndll dllgsldlsa ptptstlpqt qsptqifnpa nttnflvdgl lnssqsiqnd
      721 tpvmivldka glkitfrler psdvndllvi nmsaqnsgsa iltdflfqaa vpktfqlqml
      781 spsstvipps gqvtqvlkva ninkvplrmr lrisytgpag pvleqtevnn fppitsq
//
LOCUS       XP_034186511             837 aa            linear   INV 03-SEP-2025
DEFINITION  adaptor protein complex 1, gamma subunit isoform X9 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186511
VERSION     XP_034186511.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330620.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186511.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..837
                     /product="adaptor protein complex 1, gamma subunit isoform
                     X9"
                     /calculated_mol_wt=93040
     Region          23..583
                     /region_name="Adaptin_N"
                     /note="Adaptin N terminal region; pfam01602"
                     /db_xref="CDD:396262"
     Region          726..832
                     /region_name="Alpha_adaptinC2"
                     /note="Adaptin C-terminal domain; smart00809"
                     /db_xref="CDD:197886"
     CDS             1..837
                     /gene="AP-1gamma"
                     /coded_by="XM_034330620.2:382..2895"
                     /db_xref="GeneID:117607205"
ORIGIN      
        1 mpaptrlrdl irqiraarta aeertvvnke cayirstfre edsvwrcrni akllyihmlg
       61 ypahfgqlec lkliasprft dkrigylgam lllderqdvh llitnclknd lnsstqfvig
      121 lalctlgaia spemardlas everlmkspn ayirkkaalc afriirrvpe lmemflpatr
      181 sliteknhgv litgvtlite mcensvdtln hfkkecghre ivpnlvrilk nlilagyspe
      241 hdvsgvsdpf lqvkilrllr ilgrndidas eamndilaqv atntetsknv gntilyetvl
      301 simdiksesg lrvlavnilg rfllnndkni ryvalntllk tvyvdtsavq rhrstilecl
      361 kdpdvsirrr amelsfalvn snnirnmmke lllfleradp efkaqcssni vmsaerfapn
      421 krwhletlfk vlvaagnyvr ddvvactiql isetqaqqsy avsalwkale kdtadkqpla
      481 qvatwcigey gdlllyapps edvdapvnlt edevidvyqr llwspqntvv tkqytllslt
      541 klstrfqkgn ekirqiidtf gsnlhielqq rgiefsqlfr kydhlrsall ermppmetar
      601 pqangiigma ngepepeeek staleastpp sdssalldll gttdlgmtps vssknppans
      661 tavvnnndll dllgsldlsa ptptstlpqt qsptqifnpa nttnflvdgl lnssqsiqnd
      721 tpvmivldka glkitfrler psdvndllvi nmsaqnsgsa iltdflfqaa vpktfqlqml
      781 spsstvipps gqvtqvlkva ninkvplrmr lrisytgpag pvleqtevnn fppitsq
//
LOCUS       XP_034187218             692 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187218
VERSION     XP_034187218.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331327.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..692
                     /product="uncharacterized protein LOC117607534 isoform X1"
                     /calculated_mol_wt=76018
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          618..670
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(618,621,639,642,645,648,666,669)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..692
                     /gene="LOC117607534"
                     /coded_by="XM_034331327.2:312..2390"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrpksp vcrvrsrrcy ssdraecadg
      601 gccrpksgce clgggldcqr cgrkvyqaem qivsgipfhn icfscyccrk plesltyqen
      661 cgeiyckqcy vrnfgpqgyg ygvgpgalqt pm
//
LOCUS       XP_034187220             692 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187220
VERSION     XP_034187220.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331329.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..692
                     /product="uncharacterized protein LOC117607534 isoform X1"
                     /calculated_mol_wt=76018
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          618..670
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(618,621,639,642,645,648,666,669)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..692
                     /gene="LOC117607534"
                     /coded_by="XM_034331329.2:187..2265"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrpksp vcrvrsrrcy ssdraecadg
      601 gccrpksgce clgggldcqr cgrkvyqaem qivsgipfhn icfscyccrk plesltyqen
      661 cgeiyckqcy vrnfgpqgyg ygvgpgalqt pm
//
LOCUS       XP_034187221             692 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187221
VERSION     XP_034187221.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331330.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..692
                     /product="uncharacterized protein LOC117607534 isoform X1"
                     /calculated_mol_wt=76018
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          618..670
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(618,621,639,642,645,648,666,669)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..692
                     /gene="LOC117607534"
                     /coded_by="XM_034331330.2:300..2378"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrpksp vcrvrsrrcy ssdraecadg
      601 gccrpksgce clgggldcqr cgrkvyqaem qivsgipfhn icfscyccrk plesltyqen
      661 cgeiyckqcy vrnfgpqgyg ygvgpgalqt pm
//
LOCUS       XP_034187222             681 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187222
VERSION     XP_034187222.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331331.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..681
                     /product="uncharacterized protein LOC117607534 isoform X2"
                     /calculated_mol_wt=74752
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          607..659
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(607,610,628,631,634,637,655,658)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..681
                     /gene="LOC117607534"
                     /coded_by="XM_034331331.2:312..2357"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrrcys sdraecadgg ccrpksgcec
      601 lgggldcqrc grkvyqaemq ivsgipfhni cfscyccrkp lesltyqenc geiyckqcyv
      661 rnfgpqgygy gvgpgalqtp m
//
LOCUS       XP_034187223             680 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187223
VERSION     XP_034187223.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331332.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..680
                     /product="uncharacterized protein LOC117607534 isoform X3"
                     /calculated_mol_wt=74596
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          606..658
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(606,609,627,630,633,636,654,657)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..680
                     /gene="LOC117607534"
                     /coded_by="XM_034331332.2:312..2354"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrcyss draecadggc crpksgcecl
      601 gggldcqrcg rkvyqaemqi vsgipfhnic fscyccrkpl esltyqencg eiyckqcyvr
      661 nfgpqgygyg vgpgalqtpm
//
LOCUS       XP_034187224             677 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187224
VERSION     XP_034187224.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331333.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..677
                     /product="uncharacterized protein LOC117607534 isoform X4"
                     /calculated_mol_wt=74242
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          603..655
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(603,606,624,627,630,633,651,654)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..677
                     /gene="LOC117607534"
                     /coded_by="XM_034331333.2:312..2345"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrsdra ecadggccrp ksgceclggg
      601 ldcqrcgrkv yqaemqivsg ipfhnicfsc yccrkplesl tyqencgeiy ckqcyvrnfg
      661 pqgygygvgp galqtpm
//
LOCUS       XP_034187225             668 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X5 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187225
VERSION     XP_034187225.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331334.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..668
                     /product="uncharacterized protein LOC117607534 isoform X5"
                     /calculated_mol_wt=73532
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          618..667
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(618,621,639,642,645,648,666)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..668
                     /gene="LOC117607534"
                     /coded_by="XM_034331334.2:312..2318"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrpksp vcrvrsrrcy ssdraecadg
      601 gccrpksgce clgggldcqr cgrkvyqaem qivsgipfhn icfscyccrk plesltyqen
      661 cgeiyckr
//
LOCUS       XP_034187226             667 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X6 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187226
VERSION     XP_034187226.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331335.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..667
                     /product="uncharacterized protein LOC117607534 isoform X6"
                     /calculated_mol_wt=73136
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          593..645
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(593,596,614,617,620,623,641,644)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..667
                     /gene="LOC117607534"
                     /coded_by="XM_034331335.2:312..2315"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsgnsit ipsrrkfayp
      241 pvppprsplp srrrvsfcdv vfgdtrgneq ncimkmqnah lrpccnnnee ytydiwqadd
      301 tagknhggye nvkinhtedt grmhfeksts dccdgeedss skcneleknr gqdrcagnte
      361 lhpyrtmndk dgsskeetit cthqsgpnss ndlddsermr ggcggpcgpr prilcgtlkp
      421 vetichctkr epepcrtvvt scacsspcid rrgccpsvtd rpickkpiir crqpctvesp
      481 gcgsggccgs gcrggcgkpg qtclpsrqca ippcrpcspc ssprpcppqp icccrkcgnc
      541 cgsgggtscc rpkspvcrvr srrcyssdra ecadggccrp ksgceclggg ldcqrcgrkv
      601 yqaemqivsg ipfhnicfsc yccrkplesl tyqencgeiy ckqcyvrnfg pqgygygvgp
      661 galqtpm
//
LOCUS       XP_034187228             650 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X7 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187228
VERSION     XP_034187228.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331337.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..650
                     /product="uncharacterized protein LOC117607534 isoform X7"
                     /calculated_mol_wt=71564
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     CDS             1..650
                     /gene="LOC117607534"
                     /coded_by="XM_034331337.2:312..2264"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallsadktr vtsisrnkki
      241 nkydpvcefs gnsitipsrr kfayppvppp rsplpsrrrv sfcdvvfgdt rgneqncimk
      301 mqnahlrpcc nnneeytydi wqaddtagkn hggyenvkin htedtgrmhf ekstsdccdg
      361 eedssskcne leknrgqdrc agntelhpyr tmndkdgssk eetitcthqs gpnssndldd
      421 sermrggcgg pcgprprilc gtlkpvetic hctkrepepc rtvvtscacs spcidrrgcc
      481 psvtdrpick kpiircrqpc tvespgcgsg gccgsgcrgg cgkpgqtclp srqcaippcr
      541 pcspcssprp cppqpicccr kcgnccgsgg gtsccrpksp vcrvrsrrcy ssdraecadg
      601 gccrpksgce clgggldcqr cgrkvyqaem qdlqikwaes rtrrmvvflc
//
LOCUS       XP_034187229             564 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607534 isoform X8 [Osmia lignaria
            lignaria].
ACCESSION   XP_034187229
VERSION     XP_034187229.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331338.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..564
                     /product="uncharacterized protein LOC117607534 isoform X8"
                     /calculated_mol_wt=61342
     Region          5..57
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(5,8,26,29,32,35,53,56)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188788"
     Region          490..542
                     /region_name="LIM"
                     /note="LIM is a small protein-protein interaction domain,
                     containing two zinc fingers; cl02475"
                     /db_xref="CDD:413332"
     Site            order(490,493,511,514,517,520,538,541)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:188712"
     CDS             1..564
                     /gene="LOC117607534"
                     /coded_by="XM_034331338.2:312..2006"
                     /db_xref="GeneID:117607534"
ORIGIN      
        1 mpgvcprcnr evyfaeekla lgkvwhtfcf sccncrklln scnvvtylge lfckscyvrl
       61 apsitnheik gtsqllgtlt fsnqesngyc cpvensigna slnreqhyqt gkcrlrgggs
      121 eveetnvcld eekkhflene yanvgivnsm tticdpppsp tavttwynrq hcklrstlsl
      181 esqknnavak dyrersevqe aesnqselkt afsndevnit eallstsdcc dgeedssskc
      241 neleknrgqd rcagntelhp yrtmndkdgs skeetitcth qsgpnssndl ddsermrggc
      301 ggpcgprpri lcgtlkpvet ichctkrepe pcrtvvtsca csspcidrrg ccpsvtdrpi
      361 ckkpiircrq pctvespgcg sggccgsgcr ggcgkpgqtc lpsrqcaipp crpcspcssp
      421 rpcppqpicc crkcgnccgs gggtsccrpk spvcrvrsrr cyssdraeca dggccrpksg
      481 ceclgggldc qrcgrkvyqa emqivsgipf hnicfscycc rkplesltyq encgeiyckq
      541 cyvrnfgpqg ygygvgpgal qtpm
//
LOCUS       XP_076548261             617 aa            linear   INV 03-SEP-2025
DEFINITION  bicoid stability factor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548261
VERSION     XP_076548261.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692146.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..617
                     /product="bicoid stability factor isoform X1"
                     /calculated_mol_wt=72392
     Region          136..163
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          171..202
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          207..234
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          240..264
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     CDS             1..617
                     /gene="bsf"
                     /coded_by="XM_076692146.1:348..2201"
                     /db_xref="GeneID:117607210"
ORIGIN      
        1 mtnmllrcvn yfgylpkvai lnenkkiisl avqqcaklli ltrstsqlid enkiaidekf
       61 hniskniliy ksirkneied ivnriqspyl ltsyqafsll rwcnnvedct pqervkimks
      121 lwtnlticnf qlevihynai lklyvdnnyd islsdllmem knknidpnsv thlicfeyyc
      181 mkgdinksll llksyfyrfp fsslepifna llmgfsqlgn ieyvnkllnn mtnkdiklts
      241 ksytaimcty aklknidkin eviktcysnn myftnanile viyvlavnnh vehinsmyqy
      301 lkkvlyvsde eiqfilklin inqaniavta flhmilkvdk wsqyeniiki iikhvvdrym
      361 aadlvvqicn cfksnpgmyk ksvslslyys lqkddhlfls llraykshyi ikphyfwpll
      421 frratkydhl gilnilqimv eefnvfpcih titdyvlpts fkslhhtkdl lkqkykvdvt
      481 vfnnawvllc lkkyktgyaa symqynpgpy fykelvydlr natifrndie sfvsisynll
      541 entnnaklel vsmdkqlcdm mlefplnkiw lkrivdrltr kgielkdtti kminiflkey
      601 egndgtpsnk trnvqin
//
LOCUS       XP_034186519             617 aa            linear   INV 03-SEP-2025
DEFINITION  bicoid stability factor isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186519
VERSION     XP_034186519.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330628.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186519.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..617
                     /product="bicoid stability factor isoform X1"
                     /calculated_mol_wt=72392
     Region          136..163
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          171..202
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          207..234
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          240..264
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     CDS             1..617
                     /gene="bsf"
                     /coded_by="XM_034330628.2:499..2352"
                     /db_xref="GeneID:117607210"
ORIGIN      
        1 mtnmllrcvn yfgylpkvai lnenkkiisl avqqcaklli ltrstsqlid enkiaidekf
       61 hniskniliy ksirkneied ivnriqspyl ltsyqafsll rwcnnvedct pqervkimks
      121 lwtnlticnf qlevihynai lklyvdnnyd islsdllmem knknidpnsv thlicfeyyc
      181 mkgdinksll llksyfyrfp fsslepifna llmgfsqlgn ieyvnkllnn mtnkdiklts
      241 ksytaimcty aklknidkin eviktcysnn myftnanile viyvlavnnh vehinsmyqy
      301 lkkvlyvsde eiqfilklin inqaniavta flhmilkvdk wsqyeniiki iikhvvdrym
      361 aadlvvqicn cfksnpgmyk ksvslslyys lqkddhlfls llraykshyi ikphyfwpll
      421 frratkydhl gilnilqimv eefnvfpcih titdyvlpts fkslhhtkdl lkqkykvdvt
      481 vfnnawvllc lkkyktgyaa symqynpgpy fykelvydlr natifrndie sfvsisynll
      541 entnnaklel vsmdkqlcdm mlefplnkiw lkrivdrltr kgielkdtti kminiflkey
      601 egndgtpsnk trnvqin
//
LOCUS       XP_076548262             611 aa            linear   INV 03-SEP-2025
DEFINITION  bicoid stability factor isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548262
VERSION     XP_076548262.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692147.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..611
                     /product="bicoid stability factor isoform X2"
                     /calculated_mol_wt=71667
     Region          136..163
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          171..202
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          207..234
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          240..264
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     CDS             1..611
                     /gene="bsf"
                     /coded_by="XM_076692147.1:351..2186"
                     /db_xref="GeneID:117607210"
ORIGIN      
        1 mtnmllrcvn yfgylpkvai lnenkkiisl avqqcaklli ltrstsqlid enkiaidekf
       61 hniskniliy ksirkneied ivnriqspyl ltsyqafsll rwcnnvedct pqervkimks
      121 lwtnlticnf qlevihynai lklyvdnnyd islsdllmem knknidpnsv thlicfeyyc
      181 mkgdinksll llksyfyrfp fsslepifna llmgfsqlgn ieyvnkllnn mtnkdiklts
      241 ksytaimcty aklknidkin eviktcysnn myftnanile viyvlavnnh vehinsmyqy
      301 lkkvlyvsde eiqfilklin inqaniavta flhmilkvdk wsqyeniiki iikhvvdrym
      361 aadlvvqicn cfksnpgmyk ksvslslyys lqkddhlfls llraykshyi ikphyfwpll
      421 frratkydhl gilnilqimv eefnvfpcih titdyvlpts fkslhhtkdl lkqkykvdvt
      481 vfnnawvllc lkkyktgyaa symqynpgpy fykelvydlr natifrndie sfvsisynll
      541 entnnaklel vsmdkqlcdm mlefplnkiw lkrivdrltr kgielkdtti kminiflkey
      601 egndgtpsnk t
//
LOCUS       XP_076548263             576 aa            linear   INV 03-SEP-2025
DEFINITION  bicoid stability factor isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076548263
VERSION     XP_076548263.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692148.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..576
                     /product="bicoid stability factor isoform X3"
                     /calculated_mol_wt=67553
     Region          136..163
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          171..202
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          207..234
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          240..264
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     CDS             1..576
                     /gene="bsf"
                     /coded_by="XM_076692148.1:351..2081"
                     /db_xref="GeneID:117607210"
ORIGIN      
        1 mtnmllrcvn yfgylpkvai lnenkkiisl avqqcaklli ltrstsqlid enkiaidekf
       61 hniskniliy ksirkneied ivnriqspyl ltsyqafsll rwcnnvedct pqervkimks
      121 lwtnlticnf qlevihynai lklyvdnnyd islsdllmem knknidpnsv thlicfeyyc
      181 mkgdinksll llksyfyrfp fsslepifna llmgfsqlgn ieyvnkllnn mtnkdiklts
      241 ksytaimcty aklknidkin eviktcysnn myftnanile viyvlavnnh vehinsmyqy
      301 lkkvlyvsde eiqfilklin inqaniavta flhmilkvdk wsqyeniiki iikhvvdrym
      361 aadlvvqicn cfksnpgmyk ksvslslyys lqkddhlfls llraykshyi ikphyfwpll
      421 frratkydhl gilnilqimv eefnvfpcih titdyvlpts fkslhhtkdl lkqkykvdvt
      481 vfnnawvllc lkkyktgyaa symqynpgpy fykelvydlr natifrndie sfivdrltrk
      541 gielkdttik miniflkeye gndgtpsnkt rnvqin
//
LOCUS       XP_076548264             570 aa            linear   INV 03-SEP-2025
DEFINITION  bicoid stability factor isoform X4 [Osmia lignaria lignaria].
ACCESSION   XP_076548264
VERSION     XP_076548264.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692149.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..570
                     /product="bicoid stability factor isoform X4"
                     /calculated_mol_wt=66829
     Region          136..163
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          171..202
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          207..234
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     Region          240..264
                     /region_name="PPR repeat"
                     /note="PPR repeat [structural motif]"
                     /db_xref="CDD:276811"
     CDS             1..570
                     /gene="bsf"
                     /coded_by="XM_076692149.1:354..2066"
                     /db_xref="GeneID:117607210"
ORIGIN      
        1 mtnmllrcvn yfgylpkvai lnenkkiisl avqqcaklli ltrstsqlid enkiaidekf
       61 hniskniliy ksirkneied ivnriqspyl ltsyqafsll rwcnnvedct pqervkimks
      121 lwtnlticnf qlevihynai lklyvdnnyd islsdllmem knknidpnsv thlicfeyyc
      181 mkgdinksll llksyfyrfp fsslepifna llmgfsqlgn ieyvnkllnn mtnkdiklts
      241 ksytaimcty aklknidkin eviktcysnn myftnanile viyvlavnnh vehinsmyqy
      301 lkkvlyvsde eiqfilklin inqaniavta flhmilkvdk wsqyeniiki iikhvvdrym
      361 aadlvvqicn cfksnpgmyk ksvslslyys lqkddhlfls llraykshyi ikphyfwpll
      421 frratkydhl gilnilqimv eefnvfpcih titdyvlpts fkslhhtkdl lkqkykvdvt
      481 vfnnawvllc lkkyktgyaa symqynpgpy fykelvydlr natifrndie sfivdrltrk
      541 gielkdttik miniflkeye gndgtpsnkt
//
LOCUS       XP_034186520             585 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 5-like isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186520
VERSION     XP_034186520.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330629.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..585
                     /product="monocarboxylate transporter 5-like isoform X1"
                     /calculated_mol_wt=64882
     Region          70..562
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(81..82,85..86,89,124,175..176,178..180,183,202,
                     205..206,209,393,396..397,400..402,405,432,436,487..488,
                     492,496,512,515..516,519..520,523)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..585
                     /gene="LOC117607211"
                     /coded_by="XM_034330629.2:321..2078"
                     /db_xref="GeneID:117607211"
ORIGIN      
        1 miinmtfrcl llretftkfd ncnsmahatv sssvetkqqd eengnenvtc nsmdcrgtst
       61 imeppdggwg yfvvlasfli hviadgvtys fgifylefly yfeegkgtta wiasilvgvt
      121 lcsgpisslf vnkygcrvvt ivgsilasvs lfasmwaqnv vtlyftigig tglgfgliyl
      181 paivsvtcyf ekyrslatgi avcgsglgtl ifapclkyli seygwrgaim icsgivlnci
      241 vlgalfrple tnkrkkkssp ekcsknflrk qseeyletlp kvenniklys isfkkenntv
      301 dvksqsqpvl iskdvihqdn lenihkehln ftqrysgeml qvyesstsfp skaehikskn
      361 skvhskkqtn tlqeildisl lknpvfilfi fsnfctsigf yvpyvyvlpq aeeqgidknd
      421 asyllavigi antvgriilg yvsdkpwvnr lliynlclti cgvstmlstl ctsfisftfy
      481 ssifgftsga yvgltsvilv dllglnyltn afgqlllfqg fasflgppia gwlydalqsy
      541 npgfftaggm itvsglilff ipfiqqkser nvnqreknti nnefv
//
LOCUS       XP_076548265             577 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 5-like isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548265
VERSION     XP_076548265.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692150.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..577
                     /product="monocarboxylate transporter 5-like isoform X2"
                     /calculated_mol_wt=63795
     Region          62..554
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(73..74,77..78,81,116,167..168,170..172,175,194,
                     197..198,201,385,388..389,392..394,397,424,428,479..480,
                     484,488,504,507..508,511..512,515)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..577
                     /gene="LOC117607211"
                     /coded_by="XM_076692150.1:176..1909"
                     /db_xref="GeneID:117607211"
ORIGIN      
        1 mitnnvssfm fdncnsmaha tvsssvetkq qdeengnenv tcnsmdcrgt stimeppdgg
       61 wgyfvvlasf lihviadgvt ysfgifylef lyyfeegkgt tawiasilvg vtlcsgpiss
      121 lfvnkygcrv vtivgsilas vslfasmwaq nvvtlyftig igtglgfgli ylpaivsvtc
      181 yfekyrslat giavcgsglg tlifapclky liseygwrga imicsgivln civlgalfrp
      241 letnkrkkks spekcsknfl rkqseeylet lpkvennikl ysisfkkenn tvdvksqsqp
      301 vliskdvihq dnlenihkeh lnftqrysge mlqvyessts fpskaehiks knskvhskkq
      361 tntlqeildi sllknpvfil fifsnfctsi gfyvpyvyvl pqaeeqgidk ndasyllavi
      421 giantvgrii lgyvsdkpwv nrlliynlcl ticgvstmls tlctsfisft fyssifgfts
      481 gayvgltsvi lvdllglnyl tnafgqlllf qgfasflgpp iagwlydalq synpgfftag
      541 gmitvsglil ffipfiqqks ernvnqrekn tinnefv
//
LOCUS       XP_076548266             512 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 5-like isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548266
VERSION     XP_076548266.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692151.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..512
                     /product="monocarboxylate transporter 5-like isoform X3"
                     /calculated_mol_wt=56991
     Region          51..489
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(51,102..103,105..107,110,129,132..133,136,320,
                     323..324,327..329,332,359,363,414..415,419,423,439,
                     442..443,446..447,450)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..512
                     /gene="LOC117607211"
                     /coded_by="XM_076692151.1:524..2062"
                     /db_xref="GeneID:117607211"
ORIGIN      
        1 mglfcgsril lnscncgrsy vflrnfllgi fvlfrggkry hrvdcfdisr gpisslfvnk
       61 ygcrvvtivg silasvslfa smwaqnvvtl yftigigtgl gfgliylpai vsvtcyfeky
      121 rslatgiavc gsglgtlifa pclkylisey gwrgaimics givlncivlg alfrpletnk
      181 rkkksspekc sknflrkqse eyletlpkve nniklysisf kkenntvdvk sqsqpvlisk
      241 dvihqdnlen ihkehlnftq rysgemlqvy esstsfpska ehiksknskv hskkqtntlq
      301 eildisllkn pvfilfifsn fctsigfyvp yvyvlpqaee qgidkndasy llavigiant
      361 vgriilgyvs dkpwvnrlli ynlclticgv stmlstlcts fisftfyssi fgftsgayvg
      421 ltsvilvdll glnyltnafg qlllfqgfas flgppiagwl ydalqsynpg fftaggmitv
      481 sglilffipf iqqksernvn qrekntinne fv
//
LOCUS       XP_076548267             499 aa            linear   INV 03-SEP-2025
DEFINITION  monocarboxylate transporter 5-like isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548267
VERSION     XP_076548267.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692152.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..499
                     /product="monocarboxylate transporter 5-like isoform X4"
                     /calculated_mol_wt=55637
     Region          38..476
                     /region_name="MFS_MCT_SLC16"
                     /note="Monocarboxylate transporter (MCT) family of the
                     Major Facilitator Superfamily of transporters; cd17352"
                     /db_xref="CDD:340910"
     Site            order(38,89..90,92..94,97,116,119..120,123,307,310..311,
                     314..316,319,346,350,401..402,406,410,426,429..430,
                     433..434,437)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340910"
     CDS             1..499
                     /gene="LOC117607211"
                     /coded_by="XM_076692152.1:379..1878"
                     /db_xref="GeneID:117607211"
ORIGIN      
        1 mldgrsyvfl rnfllgifvl frggkryhrv dcfdisrgpi sslfvnkygc rvvtivgsil
       61 asvslfasmw aqnvvtlyft igigtglgfg liylpaivsv tcyfekyrsl atgiavcgsg
      121 lgtlifapcl kyliseygwr gaimicsgiv lncivlgalf rpletnkrkk ksspekcskn
      181 flrkqseeyl etlpkvenni klysisfkke nntvdvksqs qpvliskdvi hqdnlenihk
      241 ehlnftqrys gemlqvyess tsfpskaehi ksknskvhsk kqtntlqeil disllknpvf
      301 ilfifsnfct sigfyvpyvy vlpqaeeqgi dkndasylla vigiantvgr iilgyvsdkp
      361 wvnrlliynl clticgvstm lstlctsfis ftfyssifgf tsgayvglts vilvdllgln
      421 yltnafgqll lfqgfasflg ppiagwlyda lqsynpgfft aggmitvsgl ilffipfiqq
      481 ksernvnqre kntinnefv
//
LOCUS       XP_034187230             534 aa            linear   INV 03-SEP-2025
DEFINITION  homeobox protein Dbx [Osmia lignaria lignaria].
ACCESSION   XP_034187230
VERSION     XP_034187230.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331339.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="homeobox protein Dbx"
                     /calculated_mol_wt=59743
     Region          414..468
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..534
                     /gene="Dbx"
                     /coded_by="XM_034331339.2:818..2422"
                     /db_xref="GeneID:117607536"
ORIGIN      
        1 mfpssaamlk nlqqsamtsp flmenllqsk aspgadltsl tlnwaaslva rqrerecean
       61 lriarekvsp ssanqdqldq rsssgtrgsd rgnpmidcrd hqrgsgridr qndriqilrh
      121 keemernhia yvdvdnerid ghvivdrlca merlcnerie nrsnsavdsc nsnadedpts
      181 lemdgglhgd rekedgmlgg ervttlqdrr ydlgcrnvih tsdemtiqge revavervvd
      241 rigertsacs cgdeqclgpa crtiqekekp qlkfsvnail ggnhdrrphs dnfqslppea
      301 ipaflqnlqn sanyniakpi arpaayhpyh rpshqppqrh lppnahhtlq qlfyrgpylt
      361 vassgtgghh pgapgggtaf pgaiqgslgd fastglvfpw atnargkprr gmmrravfsd
      421 lqrrglekrf qiqkyiskpd rkklaeklgl kdsqvkiwfq nrrmkwrnsk erellatggs
      481 reqtlpnknn pnpdlsdadg drprmdlsdv spltspqrpe eqteneedee invt
//
LOCUS       XP_034187232             534 aa            linear   INV 03-SEP-2025
DEFINITION  homeobox protein Dbx [Osmia lignaria lignaria].
ACCESSION   XP_034187232
VERSION     XP_034187232.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331341.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="homeobox protein Dbx"
                     /calculated_mol_wt=59743
     Region          414..468
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..534
                     /gene="Dbx"
                     /coded_by="XM_034331341.2:718..2322"
                     /db_xref="GeneID:117607536"
ORIGIN      
        1 mfpssaamlk nlqqsamtsp flmenllqsk aspgadltsl tlnwaaslva rqrerecean
       61 lriarekvsp ssanqdqldq rsssgtrgsd rgnpmidcrd hqrgsgridr qndriqilrh
      121 keemernhia yvdvdnerid ghvivdrlca merlcnerie nrsnsavdsc nsnadedpts
      181 lemdgglhgd rekedgmlgg ervttlqdrr ydlgcrnvih tsdemtiqge revavervvd
      241 rigertsacs cgdeqclgpa crtiqekekp qlkfsvnail ggnhdrrphs dnfqslppea
      301 ipaflqnlqn sanyniakpi arpaayhpyh rpshqppqrh lppnahhtlq qlfyrgpylt
      361 vassgtgghh pgapgggtaf pgaiqgslgd fastglvfpw atnargkprr gmmrravfsd
      421 lqrrglekrf qiqkyiskpd rkklaeklgl kdsqvkiwfq nrrmkwrnsk erellatggs
      481 reqtlpnknn pnpdlsdadg drprmdlsdv spltspqrpe eqteneedee invt
//
LOCUS       XP_034187233             534 aa            linear   INV 03-SEP-2025
DEFINITION  homeobox protein Dbx [Osmia lignaria lignaria].
ACCESSION   XP_034187233
VERSION     XP_034187233.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331342.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..534
                     /product="homeobox protein Dbx"
                     /calculated_mol_wt=59743
     Region          414..468
                     /region_name="Homeodomain"
                     /note="pfam00046"
                     /db_xref="CDD:459649"
     CDS             1..534
                     /gene="Dbx"
                     /coded_by="XM_034331342.2:799..2403"
                     /db_xref="GeneID:117607536"
ORIGIN      
        1 mfpssaamlk nlqqsamtsp flmenllqsk aspgadltsl tlnwaaslva rqrerecean
       61 lriarekvsp ssanqdqldq rsssgtrgsd rgnpmidcrd hqrgsgridr qndriqilrh
      121 keemernhia yvdvdnerid ghvivdrlca merlcnerie nrsnsavdsc nsnadedpts
      181 lemdgglhgd rekedgmlgg ervttlqdrr ydlgcrnvih tsdemtiqge revavervvd
      241 rigertsacs cgdeqclgpa crtiqekekp qlkfsvnail ggnhdrrphs dnfqslppea
      301 ipaflqnlqn sanyniakpi arpaayhpyh rpshqppqrh lppnahhtlq qlfyrgpylt
      361 vassgtgghh pgapgggtaf pgaiqgslgd fastglvfpw atnargkprr gmmrravfsd
      421 lqrrglekrf qiqkyiskpd rkklaeklgl kdsqvkiwfq nrrmkwrnsk erellatggs
      481 reqtlpnknn pnpdlsdadg drprmdlsdv spltspqrpe eqteneedee invt
//
LOCUS       XP_034186523             502 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein SmydA-8 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186523
VERSION     XP_034186523.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330632.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="SET domain-containing protein SmydA-8 isoform
                     X1"
                     /calculated_mol_wt=57337
     Region          34..>75
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Region          <195..285
                     /region_name="SET_SMYD"
                     /note="SET domain (including SET domain and post-SET
                     domain) found in SET and MYND domain-containing protein,
                     and similar proteins; cd20071"
                     /db_xref="CDD:380997"
     Site            order(196..197,210,220..224,258,260)
                     /site_type="active"
                     /db_xref="CDD:380997"
     Site            order(196..197,210,220,257..258,260)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380997"
     Site            order(226,280,282,285)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380997"
     CDS             1..502
                     /gene="LOC117607212"
                     /coded_by="XM_034330632.2:461..1969"
                     /db_xref="GeneID:117607212"
ORIGIN      
        1 msfqdivepe kveellvshl kesniacedp rpwtvrysnl ggrgmfatrd iapnelifid
       61 apmvigprcv gkhlqmcacc yknecplfpc drgcslpvcs sqcenspkhv nyeceylrsl
      121 vptcgtdwsl nlllalipir alfmtelqre clralqcdqt itsnyeiell lrtverspne
      181 kdmelmkrvc gafnsnsfet vsvhdkdrss slrglyplgg lqnhncvpnt rhhfdgkyrl
      241 yvnaalpiaa geeltmsytn lfwdttlrrq flritkhfsc tckrcsdpme sgtrlgallc
      301 afddcsgnll padplnvgtp wvcdrcrkti nyrqicsirs giaavteeil yksprqifkf
      361 mqkelsciip lsnylivdvk friisyygrs egiewadltd ieldtkakyc hdllsildvl
      421 ncgdckkkgl ilyelycsnl eklirfrqqn aekvqsnfsv etdenqrile kaiiilqndv
      481 vsattiehdk kyfnqfqvlf kk
//
LOCUS       XP_076548268             502 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein SmydA-8 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548268
VERSION     XP_076548268.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692153.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..502
                     /product="SET domain-containing protein SmydA-8 isoform
                     X1"
                     /calculated_mol_wt=57337
     Region          34..>75
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Region          <195..285
                     /region_name="SET_SMYD"
                     /note="SET domain (including SET domain and post-SET
                     domain) found in SET and MYND domain-containing protein,
                     and similar proteins; cd20071"
                     /db_xref="CDD:380997"
     Site            order(196..197,210,220..224,258,260)
                     /site_type="active"
                     /db_xref="CDD:380997"
     Site            order(196..197,210,220,257..258,260)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380997"
     Site            order(226,280,282,285)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380997"
     CDS             1..502
                     /gene="LOC117607212"
                     /coded_by="XM_076692153.1:431..1939"
                     /db_xref="GeneID:117607212"
ORIGIN      
        1 msfqdivepe kveellvshl kesniacedp rpwtvrysnl ggrgmfatrd iapnelifid
       61 apmvigprcv gkhlqmcacc yknecplfpc drgcslpvcs sqcenspkhv nyeceylrsl
      121 vptcgtdwsl nlllalipir alfmtelqre clralqcdqt itsnyeiell lrtverspne
      181 kdmelmkrvc gafnsnsfet vsvhdkdrss slrglyplgg lqnhncvpnt rhhfdgkyrl
      241 yvnaalpiaa geeltmsytn lfwdttlrrq flritkhfsc tckrcsdpme sgtrlgallc
      301 afddcsgnll padplnvgtp wvcdrcrkti nyrqicsirs giaavteeil yksprqifkf
      361 mqkelsciip lsnylivdvk friisyygrs egiewadltd ieldtkakyc hdllsildvl
      421 ncgdckkkgl ilyelycsnl eklirfrqqn aekvqsnfsv etdenqrile kaiiilqndv
      481 vsattiehdk kyfnqfqvlf kk
//
LOCUS       XP_076548269             429 aa            linear   INV 03-SEP-2025
DEFINITION  SET domain-containing protein SmydA-8 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_076548269
VERSION     XP_076548269.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692154.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..429
                     /product="SET domain-containing protein SmydA-8 isoform
                     X2"
                     /calculated_mol_wt=48675
     Region          34..>75
                     /region_name="SET"
                     /note="SET (Su(var)3-9, Enhancer-of-zeste, Trithorax)
                     domain superfamily; cl40432"
                     /db_xref="CDD:394802"
     Region          <195..285
                     /region_name="SET_SMYD"
                     /note="SET domain (including SET domain and post-SET
                     domain) found in SET and MYND domain-containing protein,
                     and similar proteins; cd20071"
                     /db_xref="CDD:380997"
     Site            order(196..197,210,220..224,258,260)
                     /site_type="active"
                     /db_xref="CDD:380997"
     Site            order(196..197,210,220,257..258,260)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:380997"
     Site            order(226,280,282,285)
                     /site_type="other"
                     /note="Zn binding site [ion binding]"
                     /db_xref="CDD:380997"
     CDS             1..429
                     /gene="LOC117607212"
                     /coded_by="XM_076692154.1:461..1750"
                     /db_xref="GeneID:117607212"
ORIGIN      
        1 msfqdivepe kveellvshl kesniacedp rpwtvrysnl ggrgmfatrd iapnelifid
       61 apmvigprcv gkhlqmcacc yknecplfpc drgcslpvcs sqcenspkhv nyeceylrsl
      121 vptcgtdwsl nlllalipir alfmtelqre clralqcdqt itsnyeiell lrtverspne
      181 kdmelmkrvc gafnsnsfet vsvhdkdrss slrglyplgg lqnhncvpnt rhhfdgkyrl
      241 yvnaalpiaa geeltmsytn lfwdttlrrq flritkhfsc tckrcsdpme sgtrlgallc
      301 afddcsgnll padplnvgtp wvcdrcrkti nyrqicsirs giaavteeil yksprqifkf
      361 mqkelsciip lsnylivdvk friisyygrs egiewadltd ieldtkakyc hdllsildvl
      421 ncgdckkkd
//
LOCUS       XP_034187239             428 aa            linear   INV 03-SEP-2025
DEFINITION  E3 ubiquitin-protein ligase Hakai [Osmia lignaria lignaria].
ACCESSION   XP_034187239
VERSION     XP_034187239.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331348.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..428
                     /product="E3 ubiquitin-protein ligase Hakai"
                     /calculated_mol_wt=48296
     Region          124..174
                     /region_name="RING-HC_HAKAI-like"
                     /note="RING finger, HC subclass, found in E3
                     ubiquitin-protein ligase Hakai, zinc finger protein 645
                     (ZNF645), and similar proteins; cd16508"
                     /db_xref="CDD:438171"
     Site            order(138,142..145)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:438171"
     Region          174..209
                     /region_name="zf_Hakai"
                     /note="C2H2 Hakai zinc finger domain; pfam18408"
                     /db_xref="CDD:465753"
     CDS             1..428
                     /gene="Hakai"
                     /coded_by="XM_034331348.2:159..1445"
                     /db_xref="GeneID:117607537"
ORIGIN      
        1 mmeedggkrm rgrtrgrarg rargrargrs kkqvkiiesd eedtpqqvee tpaegvgtel
       61 eeheapptqq psleqqfdle adisqleapt fttinrgppe pmlrlrwdhr vnligekvln
      121 pmihccdkcl kpiliygrmi pckhvfclsc akredkvcpr cmekvsrveq tglgtvfmct
      181 hggtrygnag crrtylsqrd lqahinhrhi sappqqvqgm qvdppqyvhk sdiesqltkv
      241 ssvamqnpri ksvqshmvqp imgndprvns mvnqspvehr qhrpqalism qnytqnsgpp
      301 ppnnaplrtn litvpiqdta itthdihtqq shhyyppppp qvnyggynvp ppvsqtqqyy
      361 pqpqhpaqvs yvpppppqqq qyvsstptsi rpsptgyiqd psygppppqq qapppqtqws
      421 qhqqqfyr
//
LOCUS       XP_034186602             416 aa            linear   INV 03-SEP-2025
DEFINITION  venom acid phosphatase Acph-1 [Osmia lignaria lignaria].
ACCESSION   XP_034186602
VERSION     XP_034186602.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330711.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186602.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..416
                     /product="venom acid phosphatase Acph-1"
                     /calculated_mol_wt=48118
     Region          26..325
                     /region_name="HP"
                     /note="Histidine phosphatase domain found in a
                     functionally diverse set of proteins, mostly phosphatases;
                     contains a His residue which is phosphorylated during the
                     reaction; cl11399"
                     /db_xref="CDD:472174"
     Site            order(35..36,39,107,282..283)
                     /site_type="active"
                     /note="catalytic core [active]"
                     /db_xref="CDD:132717"
     CDS             1..416
                     /gene="LOC117607243"
                     /coded_by="XM_034330711.2:435..1685"
                     /db_xref="GeneID:117607243"
ORIGIN      
        1 mslfdrrmmi itmlfitvam vncdlelqll hvvfrhgdkv phreyqnypn dpyrdysyyp
       61 mgsgdltnqg klreyrigtm lrerydqyfg pdywpekiya rstyiprtql slqlvlaglf
      121 ppsqkqtwnp hlpwiptssf fvpyeadnll fphhcpryre eydkflrqkn tqelvskykn
      181 vmsylskhsg ktinttssvt ymynllkeqa aqnlslpkwt etvyptpmke iialdfklrs
      241 ytrtlkrlng glllrkmied iktyqqgkle pydtkaflfs ahemnvaavv raldldepai
      301 paygatvile tlrdkkgtyy vrallwtgvs eqliiqtipg caelcpfdqf lgivkdvipk
      361 ddeyrchpge klnksekieh anssasniav gmswyssvsi vflafllapg iidtnr
//
LOCUS       XP_034187240             362 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 115 [Osmia lignaria lignaria].
ACCESSION   XP_034187240
VERSION     XP_034187240.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331349.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..362
                     /product="transmembrane protein 115"
                     /calculated_mol_wt=40420
     Region          56..158
                     /region_name="DUF1751"
                     /note="Eukaryotic integral membrane protein (DUF1751);
                     pfam08551"
                     /db_xref="CDD:369945"
     CDS             1..362
                     /gene="LOC117607538"
                     /coded_by="XM_034331349.2:1..1089"
                     /db_xref="GeneID:117607538"
ORIGIN      
        1 maaikglgrn ipylrqqfaa llgntstsvk ficivvlfsy clsfsteair vlsvtpgyll
       61 ppvfwiwtaf tfcfleihfw evcvdivtvg lcgklieplw gamemmtffa ivnfgvavls
      121 alfylflymc tndpdllfdi hihgltgyia gvavavkqim pdhillktpi gkitnrnipl
      181 mvwvmgvilw lfgllegthp tmflsgllis wiylrfyqkh nngtrgdmad nftfasffpn
      241 vlqppiavvs ntihsffvri gickkvvrrf dmsnappgli inlpgidphd serrrqialk
      301 alserlskdh tkpwqpdrak khspvppavs ipipesttpk plasplipqv svnihnhtst
      361 nt
//
LOCUS       XP_034187241             352 aa            linear   INV 03-SEP-2025
DEFINITION  prostaglandin reductase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034187241
VERSION     XP_034187241.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331350.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..352
                     /product="prostaglandin reductase 1"
                     /calculated_mol_wt=38791
     Region          25..352
                     /region_name="leukotriene_B4_DH_like"
                     /note="13-PGR is a bifunctional enzyme with delta-13
                     15-prostaglandin reductase and leukotriene B4 12
                     hydroxydehydrogenase activity; cd08294"
                     /db_xref="CDD:176254"
     Site            order(27,30..32,65,68..69,255,260..263,266,284..285,
                     288..295,298)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:176254"
     Site            order(61,145,149,170,173..175,195..196,200,216,239..240,
                     261..265,267,294..296,339..340,342,344)
                     /site_type="other"
                     /note="NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176254"
     Site            order(62,65,261,267,297)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:176254"
     CDS             1..352
                     /gene="LOC117607539"
                     /coded_by="XM_034331350.2:148..1206"
                     /db_xref="GeneID:117607539"
ORIGIN      
        1 msvftvcqrr fskmvkakkf vvvkhfqgep kstdlqlvee elppvrngef lveaeylsvd
       61 pymrpyiqry pvgvtmigsq vakiieskhp dfpvgkrvvg yfgwrthtii nqemssgndf
      121 tgqkpylvsd ladlplslyl gvlgmpgnta yfglfeickp kqgetlvvsg aagavgfhvg
      181 qlgknvaglt vigiagsdek ckwlvnelgf dhainykten vaaalrkaap kgidcyfdnv
      241 ggdisatviy qmrefgriav cgsistynsd astlpkvtil qpaivfnqlk megfivqrwa
      301 drsaegvqkn lqwlregklh yretitkgfe nmfnafvgml kgqnigkaiv qv
//
LOCUS       XP_034186533             350 aa            linear   INV 03-SEP-2025
DEFINITION  COP9 signalosome subunit 5 [Osmia lignaria lignaria].
ACCESSION   XP_034186533
VERSION     XP_034186533.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330642.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..350
                     /product="COP9 signalosome subunit 5"
                     /calculated_mol_wt=39053
     Region          47..316
                     /region_name="MPN_RPN11_CSN5"
                     /note="Mov34/MPN/PAD-1 family: proteasomal regulatory
                     protein Rpn11 and signalosome complex subunit CSN5;
                     cd08069"
                     /db_xref="CDD:163700"
     Site            order(79,141,143,151,154)
                     /site_type="active"
                     /note="MPN+ (JAMM) motif [active]"
                     /db_xref="CDD:163700"
     Site            order(141,143,154)
                     /site_type="other"
                     /note="Zinc-binding site [ion binding]"
                     /db_xref="CDD:163700"
     CDS             1..350
                     /gene="CSN5"
                     /coded_by="XM_034330642.2:1..1053"
                     /db_xref="GeneID:117607217"
ORIGIN      
        1 mastssdqst iaqktwemsn nvetistvde iyrydrkeqq dilaakpwek dhhffkdiki
       61 salallkmvm harsggtlev mglllgkvaa ntmivmdsfa lpvegtetrv naqaqayeym
      121 tayieaakqv grqenaigwy hshpgygcwl sgidvstqml nqnfqepfva ividpvrtis
      181 agkvclgafr typkgykpan eepseyqtip lnkiedfgvh ckqyyslevs yfkssldrrl
      241 ldslwnkywv ntlssssllt nadyttgqif dlsdkleqse valgrgfilg gtdphdrstv
      301 eklikatrds ckttieiihg lmaqiikdrl fnqvgsnpag cnpesqqtst
//
LOCUS       XP_034186539             334 aa            linear   INV 03-SEP-2025
DEFINITION  per1-like protein PGAP3 [Osmia lignaria lignaria].
ACCESSION   XP_034186539
VERSION     XP_034186539.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330648.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..334
                     /product="per1-like protein PGAP3"
                     /calculated_mol_wt=39921
     Region          74..315
                     /region_name="Per1"
                     /note="Per1-like family; pfam04080"
                     /db_xref="CDD:461161"
     CDS             1..334
                     /gene="PGAP3"
                     /coded_by="XM_034330648.2:153..1157"
                     /db_xref="GeneID:117607219"
ORIGIN      
        1 mhsmwqykks llewkwlcil vfqmfliant sgsigdrsqf ynlcfakcyd sncdtdenfk
       61 vkpslslrll lwsckencsy sctwdtvdyf tshglkvpqf hgkwpfirff gcqepasvif
      121 silnfyihit mywkfkrkvr stypmfyiws yfswicihgw fwsaifhard tsftevmdys
      181 cafimvltll ycmllrmtyk nnklfavitc aylstlythl shlwsgyiny dynmkfnvvv
      241 gfltfvatmv wwrrnrkrls yiyligwfni ltvlvtilev adfapifwif dahslwhast
      301 vplaillyrf miadcshlnt hyskltldid hhih
//
LOCUS       XP_034186541             268 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase mitochondrial F1 complex assembly factor 2 homolog
            l(2)k14505 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186541
VERSION     XP_034186541.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330650.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..268
                     /product="ATP synthase mitochondrial F1 complex assembly
                     factor 2 homolog l(2)k14505 isoform X1"
                     /calculated_mol_wt=30804
     Region          24..141
                     /region_name="ATP12"
                     /note="ATP12 chaperone protein; pfam07542"
                     /db_xref="CDD:462201"
     CDS             1..268
                     /gene="l(2)k14505"
                     /coded_by="XM_034330650.2:398..1204"
                     /db_xref="GeneID:117607222"
ORIGIN      
        1 mtyfkykrfv talnivrnma tvkrfyrrtn ilssgatfei tldqrklktp qgkvlqvdsk
       61 plalaiaaew dmqtetidks nmhltalsnt vidnpnnhtk envvnyivnc lemdtvlfhs
      121 nesddlyklq ikhwdplvqw fcdkygvnmt ktqsiqapav seetkkiltr hlmsynynav
      181 ygfmygvdai ksviltlaaa ermisikdav klsrleedyq tshwgsiewf hdhskydlqa
      241 rlaaailfvh lnsysvthqp knvnknvm
//
LOCUS       XP_034186542             256 aa            linear   INV 03-SEP-2025
DEFINITION  ATP synthase mitochondrial F1 complex assembly factor 2 homolog
            l(2)k14505 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186542
VERSION     XP_034186542.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330651.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..256
                     /product="ATP synthase mitochondrial F1 complex assembly
                     factor 2 homolog l(2)k14505 isoform X2"
                     /calculated_mol_wt=29223
     Region          12..129
                     /region_name="ATP12"
                     /note="ATP12 chaperone protein; pfam07542"
                     /db_xref="CDD:462201"
     CDS             1..256
                     /gene="l(2)k14505"
                     /coded_by="XM_034330651.2:105..875"
                     /db_xref="GeneID:117607222"
ORIGIN      
        1 mysnitsatv krfyrrtnil ssgatfeitl dqrklktpqg kvlqvdskpl alaiaaewdm
       61 qtetidksnm hltalsntvi dnpnnhtken vvnyivncle mdtvlfhsne sddlyklqik
      121 hwdplvqwfc dkygvnmtkt qsiqapavse etkkiltrhl msynynavyg fmygvdaiks
      181 viltlaaaer misikdavkl srleedyqts hwgsiewfhd hskydlqarl aaailfvhln
      241 sysvthqpkn vnknvm
//
LOCUS       XP_034186605             262 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 1 [Osmia lignaria lignaria].
ACCESSION   XP_034186605
VERSION     XP_034186605.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330714.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186605.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..262
                     /product="very long chain fatty acid elongase 1"
                     /calculated_mol_wt=30719
     Region          23..260
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..262
                     /gene="LOC117607245"
                     /coded_by="XM_034330714.2:545..1333"
                     /db_xref="GeneID:117607245"
ORIGIN      
        1 mdlvemfdyy wyqkadpris slplmqspit lpvilfsyly fvlkcgpkfm kdrkpynlkt
       61 fikwynifqi isnaiivqqf isagwfteit vfcepadysn tpknmkivyi lwfvlitkiv
      121 dlfetgvfvl rkknrqisfl hlyhhvstpl lvwsviryvp vapasfpmlv ncavhvimys
      181 yyllssfgek wqrilnpikp lititqmvqf ivliaysiqa fmpncnqlkp ttvlgildli
      241 infvlfysfy kstytkpark ve
//
LOCUS       XP_034186603             262 aa            linear   INV 03-SEP-2025
DEFINITION  very long chain fatty acid elongase 1-like [Osmia lignaria
            lignaria].
ACCESSION   XP_034186603
VERSION     XP_034186603.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330712.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186603.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..262
                     /product="very long chain fatty acid elongase 1-like"
                     /calculated_mol_wt=30628
     Region          23..260
                     /region_name="ELO"
                     /note="GNS1/SUR4 family; pfam01151"
                     /db_xref="CDD:460083"
     CDS             1..262
                     /gene="LOC117607244"
                     /coded_by="XM_034330712.2:280..1068"
                     /db_xref="GeneID:117607244"
ORIGIN      
        1 mdlvemfdyy wyqkadprmc rlpfmqspit lpailfsyly fvlkcgpkfm kdrkpynlkt
       61 fikwynifqi vsnaiivqqf isagwfteit vfcetadysn tpknmkivyi lwfglitkiv
      121 dlfetgvfll rkknkqisfl hlyhhvstpl icwavtrytp vagasfpmlv nstvhvimys
      181 yyllssfgek lqrilnpikp lititqmvqf ivliayaiqv fmphcnaikt psviaivnll
      241 infvlfynfy qntyvkpakk ve
//
LOCUS       XP_034186608             260 aa            linear   INV 03-SEP-2025
DEFINITION  upstream stimulatory factor 1 isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_034186608
VERSION     XP_034186608.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330717.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..260
                     /product="upstream stimulatory factor 1 isoform X1"
                     /calculated_mol_wt=29141
     Region          149..213
                     /region_name="bHLHzip_USF"
                     /note="basic Helix-Loop-Helix-zipper (bHLHzip) domain
                     found in upstream stimulatory factors, USF1, USF2 and
                     similar proteins; cd11396"
                     /db_xref="CDD:381402"
     Site            order(149,151,154..158,162,192..195)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381402"
     Site            order(161,164,167,171,198,202,205..206,208..209,213)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381402"
     CDS             1..260
                     /gene="LOC117607247"
                     /coded_by="XM_034330717.2:857..1639"
                     /db_xref="GeneID:117607247"
ORIGIN      
        1 mkmevlshfd hidesvddnt isndnvgvvl eeaeiidcdt eietvaeedn iqyqlcevnr
       61 ndnavayhvv qvgdnhtnna elslaapinn avqvltspin gqlyvlsngn evvttdstrs
      121 vaprvklqid npqtiltnik krderrrvth neverrrrdk innwisklgk llpeceqstt
      181 gdaevktnfe lqskggilar aceyitelrd vqetlsqsld enaqlleeak tlrqvvnqlr
      241 kenselkaqi snsttyilgt
//
LOCUS       XP_034186609             252 aa            linear   INV 03-SEP-2025
DEFINITION  upstream stimulatory factor 1 isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_034186609
VERSION     XP_034186609.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330718.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..252
                     /product="upstream stimulatory factor 1 isoform X2"
                     /calculated_mol_wt=28181
     Region          149..205
                     /region_name="bHLHzip_USF"
                     /note="basic Helix-Loop-Helix-zipper (bHLHzip) domain
                     found in upstream stimulatory factors, USF1, USF2 and
                     similar proteins; cd11396"
                     /db_xref="CDD:381402"
     Site            order(149,151,154..158,162,184..187)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:381402"
     Site            order(161,164,167,171,190,194,197..198,200..201,205)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:381402"
     CDS             1..252
                     /gene="LOC117607247"
                     /coded_by="XM_034330718.2:864..1622"
                     /db_xref="GeneID:117607247"
ORIGIN      
        1 mkmevlshfd hidesvddnt isndnvgvvl eeaeiidcdt eietvaeedn iqyqlcevnr
       61 ndnavayhvv qvgdnhtnna elslaapinn avqvltspin gqlyvlsngn evvttdstrs
      121 vaprvklqid npqtiltnik krderrrvth neverrrrdk innwisklgk llpeceqstt
      181 gdaeskggil araceyitel rdvqetlsqs ldenaqllee aktlrqvvnq lrkenselka
      241 qisnsttyil gt
//
LOCUS       XP_034186610             234 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607248 [Osmia lignaria lignaria].
ACCESSION   XP_034186610
VERSION     XP_034186610.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330719.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..234
                     /product="uncharacterized protein LOC117607248"
                     /calculated_mol_wt=26892
     CDS             1..234
                     /gene="LOC117607248"
                     /coded_by="XM_034330719.2:320..1024"
                     /db_xref="GeneID:117607248"
ORIGIN      
        1 mlkqlrrssy vfniargarv sfnfvrnkss edlrqtlaka ygyihrrtkm diqekmrelg
       61 dlfsktgkel sdivkqneqf iteeirkaee tlrkasevca gkhmdrlkvv ketyanrikv
      121 cndkaalgmh aarmkyegqi ektevlrnsm krilkecldt defvkciank tketarqrke
      181 igeklnetmv narisiadql rdamkchada qaeslkslge ilkdikecvv qtqq
//
LOCUS       XP_076548270             231 aa            linear   INV 03-SEP-2025
DEFINITION  AMMECR1-like protein isoform X1 [Osmia lignaria lignaria].
ACCESSION   XP_076548270
VERSION     XP_076548270.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692155.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..231
                     /product="AMMECR1-like protein isoform X1"
                     /calculated_mol_wt=26414
     Region          46..214
                     /region_name="AMMECR1"
                     /note="pfam01871"
                     /db_xref="CDD:460368"
     CDS             1..231
                     /gene="LOC143306067"
                     /coded_by="XM_076692155.1:98..793"
                     /db_xref="GeneID:143306067"
ORIGIN      
        1 maagccgtkk qklndsssvp rnnivslqng ihmrnsiivq pemgfycfdv lycqlhqldp
       61 pkppnfsnea fplfvtwtig kdmrlrgcig tfnamhlhag lreyattsaf kdsrfnpitl
      121 eelprlhvsv silrhfedgt dyldwvvgvh giriefhnek gnkrtatylp dvaieqgwnq
      181 iqtidsllhk ggykglvtpd irrslkltry qsekvtvsyq dymtrwysrr c
//
LOCUS       XP_076548271             196 aa            linear   INV 03-SEP-2025
DEFINITION  AMMECR1-like protein isoform X2 [Osmia lignaria lignaria].
ACCESSION   XP_076548271
VERSION     XP_076548271.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692156.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..196
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..196
                     /product="AMMECR1-like protein isoform X2"
                     /calculated_mol_wt=22752
     Region          37..179
                     /region_name="AMMECR1"
                     /note="pfam01871"
                     /db_xref="CDD:460368"
     CDS             1..196
                     /gene="LOC143306067"
                     /coded_by="XM_076692156.1:47..637"
                     /db_xref="GeneID:143306067"
ORIGIN      
        1 miqflasiny cnavfeteis iyfvvltyfc svfyqnplfv twtigkdmrl rgcigtfnam
       61 hlhaglreya ttsafkdsrf npitleelpr lhvsvsilrh fedgtdyldw vvgvhgirie
      121 fhnekgnkrt atylpdvaie qgwnqiqtid sllhkggykg lvtpdirrsl kltryqsekv
      181 tvsyqdymtr wysrrc
//
LOCUS       XP_076548272             194 aa            linear   INV 03-SEP-2025
DEFINITION  AMMECR1-like protein isoform X3 [Osmia lignaria lignaria].
ACCESSION   XP_076548272
VERSION     XP_076548272.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692157.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..194
                     /product="AMMECR1-like protein isoform X3"
                     /calculated_mol_wt=22266
     Region          <72..177
                     /region_name="AMMECR1"
                     /note="pfam01871"
                     /db_xref="CDD:460368"
     CDS             1..194
                     /gene="LOC143306067"
                     /coded_by="XM_076692157.1:98..682"
                     /db_xref="GeneID:143306067"
ORIGIN      
        1 maagccgtkk qklndsssvp rnnivslqng ihmrnsiivq pemgfycfdv lycqlhqldp
       61 pkppnfsnea fafkdsrfnp itleelprlh vsvsilrhfe dgtdyldwvv gvhgiriefh
      121 nekgnkrtat ylpdvaieqg wnqiqtidsl lhkggykglv tpdirrslkl tryqsekvtv
      181 syqdymtrwy srrc
//
LOCUS       XP_076548273             230 aa            linear   INV 03-SEP-2025
DEFINITION  AMMECR1-like protein [Osmia lignaria lignaria].
ACCESSION   XP_076548273
VERSION     XP_076548273.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692158.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..230
                     /product="AMMECR1-like protein"
                     /calculated_mol_wt=26120
     Region          45..214
                     /region_name="AMMECR1"
                     /note="pfam01871"
                     /db_xref="CDD:460368"
     CDS             1..230
                     /gene="LOC117607251"
                     /coded_by="XM_076692158.1:130..822"
                     /db_xref="GeneID:117607251"
ORIGIN      
        1 maagccgtkk qklnnsssvp cngsvlqngt hlrngvivkp emgffcfdvl ycqlhqldpp
       61 kspnfsneaf plfvtwtigk dmrlrgcigt fnamqlhagl reyattsafk dsrfnpitre
      121 elprlhvsvs ilrhfedgvd yldwevgvhg iriefhnekg nkrtatylpd vateqgwdqi
      181 qtidsllhkg gykghvtpdi rrsvkltryq sekitvsyqd ymthwhsrrc
//
LOCUS       XP_034186611             219 aa            linear   INV 03-SEP-2025
DEFINITION  transmembrane protein 126 [Osmia lignaria lignaria].
ACCESSION   XP_034186611
VERSION     XP_034186611.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330720.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..219
                     /product="transmembrane protein 126"
                     /calculated_mol_wt=24149
     Region          28..197
                     /region_name="TMEM126"
                     /note="Transmembrane protein 126; pfam07114"
                     /db_xref="CDD:462092"
     CDS             1..219
                     /gene="LOC117607249"
                     /coded_by="XM_034330720.2:384..1043"
                     /db_xref="GeneID:117607249"
ORIGIN      
        1 malvpgrkdt fidqnvkdgl nlsqmeaiev qkklidewep knevmalqyg sfivgttssv
       61 saqminsifr rklklrstgq fitsfglmia sggmalighe llitndilly knqcpvcyem
      121 kaslllngtg ilyplllaps lnlaiagsvg lrtphiyevk qvvgfwwnvv kpainhisla
      181 fcinsviasl vvykqhgqmn nianilkrie ekqsenslt
//
LOCUS       XP_034186544             209 aa            linear   INV 03-SEP-2025
DEFINITION  PITH domain-containing protein GA19395 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186544
VERSION     XP_034186544.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330653.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..209
                     /product="PITH domain-containing protein GA19395 isoform
                     X1"
                     /calculated_mol_wt=23625
     Region          29..173
                     /region_name="PITH"
                     /note="PITH domain; pfam06201"
                     /db_xref="CDD:461848"
     CDS             1..209
                     /gene="LOC117607224"
                     /coded_by="XM_034330653.1:106..735"
                     /db_xref="GeneID:117607224"
ORIGIN      
        1 miikrsimah qcgcgashee gelgvqynly kkidieniev lneceegsga avfkpwenrl
       61 drtkyvesdm dnellfnipf tgniklkgli iiggeddlhp nkvklyknrp ymtfddaata
      121 peqefelcvd tygvheyapk vvkfssvhhl slhftgtegs ekikiyyigl kgewspghqh
      181 gvttctyell pqmsdhmrdn ledidrais
//
LOCUS       XP_034186545             202 aa            linear   INV 03-SEP-2025
DEFINITION  PITH domain-containing protein GA19395 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186545
VERSION     XP_034186545.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330654.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="PITH domain-containing protein GA19395 isoform
                     X2"
                     /calculated_mol_wt=22783
     Region          22..166
                     /region_name="PITH"
                     /note="PITH domain; pfam06201"
                     /db_xref="CDD:461848"
     CDS             1..202
                     /gene="LOC117607224"
                     /coded_by="XM_034330654.1:235..843"
                     /db_xref="GeneID:117607224"
ORIGIN      
        1 mahqcgcgas heegelgvqy nlykkidien ievlneceeg sgaavfkpwe nrldrtkyve
       61 sdmdnellfn ipftgniklk gliiiggedd lhpnkvklyk nrpymtfdda atapeqefel
      121 cvdtygvhey apkvvkfssv hhlslhftgt egsekikiyy iglkgewspg hqhgvttcty
      181 ellpqmsdhm rdnledidra is
//
LOCUS       XP_034186546             202 aa            linear   INV 03-SEP-2025
DEFINITION  PITH domain-containing protein GA19395 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186546
VERSION     XP_034186546.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330655.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="PITH domain-containing protein GA19395 isoform
                     X2"
                     /calculated_mol_wt=22783
     Region          22..166
                     /region_name="PITH"
                     /note="PITH domain; pfam06201"
                     /db_xref="CDD:461848"
     CDS             1..202
                     /gene="LOC117607224"
                     /coded_by="XM_034330655.1:112..720"
                     /db_xref="GeneID:117607224"
ORIGIN      
        1 mahqcgcgas heegelgvqy nlykkidien ievlneceeg sgaavfkpwe nrldrtkyve
       61 sdmdnellfn ipftgniklk gliiiggedd lhpnkvklyk nrpymtfdda atapeqefel
      121 cvdtygvhey apkvvkfssv hhlslhftgt egsekikiyy iglkgewspg hqhgvttcty
      181 ellpqmsdhm rdnledidra is
//
LOCUS       XP_034186547             202 aa            linear   INV 03-SEP-2025
DEFINITION  PITH domain-containing protein GA19395 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186547
VERSION     XP_034186547.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330656.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="PITH domain-containing protein GA19395 isoform
                     X2"
                     /calculated_mol_wt=22783
     Region          22..166
                     /region_name="PITH"
                     /note="PITH domain; pfam06201"
                     /db_xref="CDD:461848"
     CDS             1..202
                     /gene="LOC117607224"
                     /coded_by="XM_034330656.1:313..921"
                     /db_xref="GeneID:117607224"
ORIGIN      
        1 mahqcgcgas heegelgvqy nlykkidien ievlneceeg sgaavfkpwe nrldrtkyve
       61 sdmdnellfn ipftgniklk gliiiggedd lhpnkvklyk nrpymtfdda atapeqefel
      121 cvdtygvhey apkvvkfssv hhlslhftgt egsekikiyy iglkgewspg hqhgvttcty
      181 ellpqmsdhm rdnledidra is
//
LOCUS       XP_034186548             202 aa            linear   INV 03-SEP-2025
DEFINITION  PITH domain-containing protein GA19395 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186548
VERSION     XP_034186548.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330657.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="PITH domain-containing protein GA19395 isoform
                     X2"
                     /calculated_mol_wt=22783
     Region          22..166
                     /region_name="PITH"
                     /note="PITH domain; pfam06201"
                     /db_xref="CDD:461848"
     CDS             1..202
                     /gene="LOC117607224"
                     /coded_by="XM_034330657.1:138..746"
                     /db_xref="GeneID:117607224"
ORIGIN      
        1 mahqcgcgas heegelgvqy nlykkidien ievlneceeg sgaavfkpwe nrldrtkyve
       61 sdmdnellfn ipftgniklk gliiiggedd lhpnkvklyk nrpymtfdda atapeqefel
      121 cvdtygvhey apkvvkfssv hhlslhftgt egsekikiyy iglkgewspg hqhgvttcty
      181 ellpqmsdhm rdnledidra is
//
LOCUS       XP_034186549             202 aa            linear   INV 03-SEP-2025
DEFINITION  PITH domain-containing protein GA19395 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186549
VERSION     XP_034186549.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330658.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..202
                     /product="PITH domain-containing protein GA19395 isoform
                     X2"
                     /calculated_mol_wt=22783
     Region          22..166
                     /region_name="PITH"
                     /note="PITH domain; pfam06201"
                     /db_xref="CDD:461848"
     CDS             1..202
                     /gene="LOC117607224"
                     /coded_by="XM_034330658.2:129..737"
                     /db_xref="GeneID:117607224"
ORIGIN      
        1 mahqcgcgas heegelgvqy nlykkidien ievlneceeg sgaavfkpwe nrldrtkyve
       61 sdmdnellfn ipftgniklk gliiiggedd lhpnkvklyk nrpymtfdda atapeqefel
      121 cvdtygvhey apkvvkfssv hhlslhftgt egsekikiyy iglkgewspg hqhgvttcty
      181 ellpqmsdhm rdnledidra is
//
LOCUS       XP_034186550             205 aa            linear   INV 03-SEP-2025
DEFINITION  ADP-ribosylation factor related protein 1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186550
VERSION     XP_034186550.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330659.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..205
                     /product="ADP-ribosylation factor related protein 1"
                     /calculated_mol_wt=23095
     Region          19..186
                     /region_name="Arfrp1"
                     /note="Arf-related protein 1 (Arfrp1); cd04160"
                     /db_xref="CDD:206725"
     Site            24..31
                     /site_type="other"
                     /note="G1 box"
                     /db_xref="CDD:206725"
     Site            order(26..32,55..56,78,134..135,137,169..171)
                     /site_type="other"
                     /note="GTP/Mg2+ binding site [chemical binding]"
                     /db_xref="CDD:206725"
     Site            order(26..27,31,35,55..62,84,89)
                     /site_type="other"
                     /note="putative GAP interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206725"
     Site            order(36..39,49..59)
                     /site_type="other"
                     /note="Switch I region"
                     /db_xref="CDD:206725"
     Site            order(56..60,65,75,79,85,87..89)
                     /site_type="other"
                     /note="putative GEF interaction site [polypeptide
                     binding]"
                     /db_xref="CDD:206725"
     Site            56
                     /site_type="other"
                     /note="G2 box"
                     /db_xref="CDD:206725"
     Site            order(57..64,74,85,88..89)
                     /site_type="active"
                     /note="putative effector interaction site [active]"
                     /db_xref="CDD:206725"
     Site            order(60..66,69..74)
                     /site_type="active"
                     /note="interswitch region [active]"
                     /db_xref="CDD:206725"
     Site            75..92
                     /site_type="other"
                     /note="Switch II region"
                     /db_xref="CDD:206725"
     Site            75..78
                     /site_type="other"
                     /note="G3 box"
                     /db_xref="CDD:206725"
     Site            134..137
                     /site_type="other"
                     /note="G4 box"
                     /db_xref="CDD:206725"
     Site            169..171
                     /site_type="other"
                     /note="G5 box"
                     /db_xref="CDD:206725"
     CDS             1..205
                     /gene="Arfrp1"
                     /coded_by="XM_034330659.1:155..772"
                     /db_xref="GeneID:117607225"
ORIGIN      
        1 mytllhglyk ylvqkdeyfi lilgldnagk ttyleaaktk ftknykgmnp skitttvgln
       61 igkidiagvr fnfwdlggqe elrslwdkyy aeshaviyiv dssdrekipe sketfdrvis
      121 sehligvpll vlankqdvpd cmgvrevkpi fnqnahligr rdcmvmpvsa lngdgvdegi
      181 hwlvdcvkrn sdvrpprnqn dncls
//
LOCUS       XP_076548274             164 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC143306068 [Osmia lignaria lignaria].
ACCESSION   XP_076548274
VERSION     XP_076548274.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692159.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..164
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..164
                     /product="uncharacterized protein LOC143306068"
                     /calculated_mol_wt=18341
     CDS             1..164
                     /gene="LOC143306068"
                     /coded_by="XM_076692159.1:2805..3299"
                     /db_xref="GeneID:143306068"
ORIGIN      
        1 mhrvgswsld dldsasmkhp srspgleiam alfgmvwllr vrsemrrcsk crheeilaaq
       61 qqqqqqqqqq lqqqqqqhhh lhhhshhqqr tvsqttsllq tpddavsssa ntlftldtpg
      121 aagsagsyce vhgfvqakeg nvdgvsvvcf qsrmnsfsfv ksff
//
LOCUS       XP_034186613             123 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate kinase isoenzyme 1-like [Osmia lignaria lignaria].
ACCESSION   XP_034186613
VERSION     XP_034186613.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330722.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186613.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="adenylate kinase isoenzyme 1-like"
                     /calculated_mol_wt=13507
     Region          51..>113
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     Site            order(55,60..63)
                     /site_type="active"
                     /db_xref="CDD:238977"
     CDS             1..123
                     /gene="LOC117607250"
                     /coded_by="XM_034330722.2:332..703"
                     /db_xref="GeneID:117607250"
ORIGIN      
        1 mpvelckpfq prskkefvkm gncfdtserd tipryesvda tplkdsqalm ifvlggpgvg
       61 kttleqkigs kimkkgfnvp adiivqlvig emlrepnayg yliigfprdk mqviicqsta
      121 gli
//
LOCUS       XP_076548275             123 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate kinase isoenzyme 1-like [Osmia lignaria lignaria].
ACCESSION   XP_076548275
VERSION     XP_076548275.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692160.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="adenylate kinase isoenzyme 1-like"
                     /calculated_mol_wt=13507
     Region          51..>113
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     Site            order(55,60..63)
                     /site_type="active"
                     /db_xref="CDD:238977"
     CDS             1..123
                     /gene="LOC117607250"
                     /coded_by="XM_076692160.1:332..703"
                     /db_xref="GeneID:117607250"
ORIGIN      
        1 mpvelckpfq prskkefvkm gncfdtserd tipryesvda tplkdsqalm ifvlggpgvg
       61 kttleqkigs kimkkgfnvp adiivqlvig emlrepnayg yliigfprdk mqviicqsta
      121 gli
//
LOCUS       XP_076548276             123 aa            linear   INV 03-SEP-2025
DEFINITION  adenylate kinase isoenzyme 1-like [Osmia lignaria lignaria].
ACCESSION   XP_076548276
VERSION     XP_076548276.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692161.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..123
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..123
                     /product="adenylate kinase isoenzyme 1-like"
                     /calculated_mol_wt=13507
     Region          51..>113
                     /region_name="NK"
                     /note="Nucleoside/nucleotide kinase (NK) is a protein
                     superfamily consisting of multiple families of enzymes
                     that share structural similarity and are functionally
                     related to the catalysis of the reversible phosphate group
                     transfer from nucleoside triphosphates...; cl17190"
                     /db_xref="CDD:450170"
     Site            order(55,60..63)
                     /site_type="active"
                     /db_xref="CDD:238977"
     CDS             1..123
                     /gene="LOC117607250"
                     /coded_by="XM_076692161.1:332..703"
                     /db_xref="GeneID:117607250"
ORIGIN      
        1 mpvelckpfq prskkefvkm gncfdtserd tipryesvda tplkdsqalm ifvlggpgvg
       61 kttleqkigs kimkkgfnvp adiivqlvig emlrepnayg yliigfprdk mqviicqsta
      121 gli
//
LOCUS       XP_034186552              90 aa            linear   INV 03-SEP-2025
DEFINITION  barrier to autointegration factor [Osmia lignaria lignaria].
ACCESSION   XP_034186552
VERSION     XP_034186552.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330661.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..90
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..90
                     /product="barrier to autointegration factor"
                     /calculated_mol_wt=9980
     Region          2..89
                     /region_name="BAF"
                     /note="Barrier to autointegration factor; pfam02961"
                     /db_xref="CDD:460766"
     CDS             1..90
                     /gene="baf"
                     /coded_by="XM_034330661.2:247..519"
                     /db_xref="GeneID:117607227"
ORIGIN      
        1 msstsqkhkn fvaepmgskp vtdlagvgev lgrrleaagf dkayvvlgqy lvlkknrelf
       61 qewmkdacsa nakqsndcyq clsdwceefl
//
LOCUS       XP_076548277            1071 aa            linear   INV 03-SEP-2025
DEFINITION  eyes shut [Osmia lignaria lignaria].
ACCESSION   XP_076548277
VERSION     XP_076548277.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_076692162.1
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1071
                     /product="eyes shut"
                     /calculated_mol_wt=116875
     Region          74..108
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          113..145
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          151..181
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          186..221
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(186,189,203)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          264..299
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          662..822
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Region          838..878
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          883..915
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          957..>1070
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cl22861"
                     /db_xref="CDD:473984"
     CDS             1..1071
                     /gene="eys"
                     /coded_by="XM_076692162.1:122..3337"
                     /db_xref="GeneID:117607203"
ORIGIN      
        1 mrrmcykrkl ivnsmkkgif ilatilllav dfsqglncst dpcmygicle dinstyscyc
       61 idgytgince inwddcwsnp clnggtcnda vaaynctcae gfvginceqr ysecsnqpcl
      121 nngtcldydg itcqcpdgys gdyceidasv cndtickngg ecmegpgfsf fcrcpegwtg
      181 rlcdedvdec vtspcknggl cinvpasytc aclfgytgkd cdkaivpcke npcrndavcl
      241 federpicyc vpdyhgilce lkyddceskf ancenggtci dgvnsftcac ptfysgpfcn
      301 vydlpstpfs tfseiseids dkemsvttpf asksstlles dmssllttst tmqsstsvng
      361 tsrlytkwyp fvettpstld rnnisifsss tssesstevt sflndvssvy sitgketsev
      421 etassesqtf ptvlsevssa pstirveteh ltqksfrnen ttasesnyhe gdartlasin
      481 dtsssetsst mgyisntgyy pvvttsveki teeattesek sngystvtss stfiefwqnk
      541 ssesstpive vpkttsqtfi dkwtsslltk asessesstf witngtsssa tstsvrsile
      601 edttvseqfs tkkslttaks ttechgmlct ssplpptrnd sncncshren cktgpsitra
      661 afngksyarq qvdvevsngn natlrifvrl rtqfkdgiil hvyfdnekya lvyleygslk
      721 fqfscgletm llgeidspid ngyetdidtk fqyfvknetn kcsarllvng ttavsgeqml
      781 plhgilprya nlhlggipmv fshhfahvsm gftgcmnslk indvprhfih dsvetfqidd
      841 cmsflclsnp cqnfgaceev ngavrcrcip gytgpfcers scdenpcamg atcisspgtg
      901 fvcvcplgth gllceedtii srpsfsvlvp gfssyvaygv snsikdamel klkliprsye
      961 qislvaylgq sgtrrdlsdh fsityvrgyi mltwdlgagv rriftsvpln aitttgtask
     1021 syiaytiqag rrgrdawlai dgignvtgrv vgsmtrldvs pilyigllss i
//
LOCUS       XP_034186335            1055 aa            linear   INV 03-SEP-2025
DEFINITION  cytosolic carboxypeptidase 2 [Osmia lignaria lignaria].
ACCESSION   XP_034186335
VERSION     XP_034186335.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330444.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034186335.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1055
                     /product="cytosolic carboxypeptidase 2"
                     /calculated_mol_wt=121233
     Region          200..327
                     /region_name="Pepdidase_M14_N"
                     /note="Cytosolic carboxypeptidase N-terminal domain;
                     pfam18027"
                     /db_xref="CDD:407865"
     Region          349..600
                     /region_name="M14_AGBL2-3_like"
                     /note="Peptidase M14-like domain of ATP/GTP binding
                     protein AGBL-2 and AGBL-3, and related proteins; cd06907"
                     /db_xref="CDD:349478"
     Site            order(394,397,444,453..454,490..491,500,564)
                     /site_type="active"
                     /db_xref="CDD:349478"
     CDS             1..1055
                     /gene="LOC117607149"
                     /coded_by="XM_034330444.2:44..3211"
                     /db_xref="GeneID:117607149"
ORIGIN      
        1 madlltgsrv kelqamlfpv qpipggalan fsklqevqll qlgrydvprr etelslfsvp
       61 fgtredqske tlrnvldsrc midgitqeaa rwptecqvlp ervrhieytr nipepfymat
      121 gkeprpkplg defgsvvfry yptsitnyfs rscvggstvl pfpsefstds sgyesketdn
      181 nnlffvarsd ttdsssdlhf esrfesgnlc kvvkitdtyy qlylrkdlyt qrhtqwyyfr
      241 isntrsrtty rlsivnlcke eslyneglrp llystedakq ravgwrrcgd niayyrndss
      301 sdeekekhtl tfnvsfphdr dtvylahcyp ytytdlqeyl aklvadpvkt rftklrllcr
      361 tiagngvyyl titaptydee mrrkrgivit arvhpgetps swtmkgiidf ltgesdqakv
      421 lrekfvfklv pmlnpdgviv gnnrcslsgk dlnrqyrtvm resypsvwht klmirrllee
      481 cgvaiycdlh ahsrkhnifv ygcqskrtns qtrlseqvfp lmlhknaadk fsfenckfhm
      541 ekgkegtgrm vmwsmgvqys ytmeasmggs qigsrsgthf ctqdyeqigr vfcetlldff
      601 dpdpvkeklr skiivrlmke gssaeeptni dltdyssdeg dtsdssvsek eetfiegsws
      661 cntppptptf lplrtfemak rrnkkgintk sylrrkrmla gravmdlptt dpgsdlcdlt
      721 dsadegvtrt edysarired yknretesne kervtdslsl peivrprsvs ldenlcvnkk
      781 esktnkrshc lhlirafrhq tpvqfnnqdi qiklsslrqq iwmgvplnva eniekridhs
      841 fakaplswgv snmastrdki nndvllkscr kklqsleyit idddtknaeg kckreeials
      901 dtspeveqqr pnsdrksrkk ksrqkwqkiv nlnskveetn rrktsfltik tdslklvtle
      961 ipsktqtrqh kidtrrkhrk npapcsisng gktsnvtksq ikshtrgqvq lqplivplcd
     1021 tsssddsnds rrqriskkkq kkkkqvkkik knvsi
//
LOCUS       XP_034187065            1001 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607456 [Osmia lignaria lignaria].
ACCESSION   XP_034187065
VERSION     XP_034187065.2
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034331174.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            On Sep 3, 2025 this sequence version replaced XP_034187065.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..1001
                     /product="uncharacterized protein LOC117607456"
                     /calculated_mol_wt=114602
     Region          5..73
                     /region_name="zf-AD"
                     /note="Zinc-finger associated domain (zf-AD); smart00868"
                     /db_xref="CDD:214871"
     Region          <294..>763
                     /region_name="rad2"
                     /note="DNA excision repair protein (rad2); TIGR00600"
                     /db_xref="CDD:273166"
     Region          <648..895
                     /region_name="PTZ00341"
                     /note="Ring-infected erythrocyte surface antigen;
                     Provisional"
                     /db_xref="CDD:173534"
     CDS             1..1001
                     /gene="LOC117607456"
                     /coded_by="XM_034331174.2:1285..4290"
                     /db_xref="GeneID:117607456"
ORIGIN      
        1 mprqccvlca sdegvfldit sdnkhvfheq feicslvevl tedqlpsklc hkcvyelnqc
       61 ssfvqrfkht tkqkklnrkr ccslcyrpar nelvfdlskd qslqhnsfdk iqkvfdydlk
      121 sfkedqkfic lscrytldvl fdlknlsket aiklndiine kvdyvnmpki ktfvvsrktt
      181 itesartvlp klqesssdtt iitrtrsqdn ksgnntksnk kqerqfcgkc kklmkieddm
      241 ykihkistsi ckkcweksgh dkneienqip qnvtepkvck vflkdvlknt eklytvnkgn
      301 qgnktyvakn knvenesksd sisnntrnsv skqskkrsis dvkdnskqtn neptlpkrsk
      361 vaakqesdsi ldskeskpsp qetrqrkatt atqnvdsdss vlimrrsgrt ltkqkratga
      421 slsdadvdrk rnrmklrnil egnaviksfe ltdesssned avprkkrrfa sispvpiekk
      481 rkkiskkdts qqtpknvfnk qlryikqskt fqqsqssesd veqvvfdttt yicdecgasy
      541 enklmglthk lthykqpkie likvkdkeva kevsdtneit deqskdqcea apirvdddee
      601 epakdmndlt enntedksyy skkeamedav dmelvmkesd ddctqtekkt desqkeeeit
      661 dtvltnsltp deaedkeqnr sssdnnndti esvekeniqs ekthntsvnn vedkeeekqn
      721 alpndsedvq egkdqeendk eeqnpeendk eeqnpeendk eeqnpeekdk eeenpeekdk
      781 eeenpeenen evndknekkd entneqvtrt erkdsseeei islgiwdese gltdkgsqkd
      841 snndtangni drtkddkcns vekkveeddv qeveinkqks serikkrtss egnkdqtvev
      901 nttividrck piivedvlek siaigdkdsd pdnvkvsksd askqeeqnec vhvaaevlqe
      961 vidlasavvq krhdvieide egnyneaetv enisreiqns v
//
LOCUS       XP_034186336             940 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607151 isoform X1 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186336
VERSION     XP_034186336.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330445.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..940
                     /product="uncharacterized protein LOC117607151 isoform X1"
                     /calculated_mol_wt=100805
     Region          755..775
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(760,762,764,766..767,770..771,774,788,790,794..795,
                     798..799,802,816,818,820,822..823,826..827,831)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          768..791
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          783..>847
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          783..804
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          783..803
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(788,794,801,803,810,817)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          795..820
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          811..832
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          811..832
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..940
                     /gene="LOC117607151"
                     /coded_by="XM_034330445.2:591..3413"
                     /db_xref="GeneID:117607151"
ORIGIN      
        1 mpkifliknr lhqqqlrlle aqhlgksppp gsgrdspigs seplslivnk hqyrdktddd
       61 rattpeslrs tspasspppq wqststptst pprrfissil ggdvpygsrg hvltraerke
      121 yssppiapss sdasqfltks eklvlprpst ppktprvepp trvsviqrvp sqnqsasrre
      181 dkvempqtqe peqeqpidya vpkrkeedee rgregakvsr aignsiarpl lamrlsgpqv
      241 vhaaaghgrs sssgnngssg ssggsnnsgs ssssssggtg nycsggavtg gsggggavgg
      301 gaggggmnpg gnggrgnygp sspptgslpp fyeslkggnn lanfanqyns tqgngyltps
      361 ptvgmecdtg qqdvnsqhsq ynaqegkqys llqnvcasyg ltlkeeedls pykiqandll
      421 sgqystydmt dtgmmvdmvt gavvdplqft atltfsspsd htalleslgd aadlflprlp
      481 aedgsndlle eslhspasag sgigqdagqm ttpvepsvdp fpehsmaltr gfdtsrhyta
      541 apqhfstskl glayttgess yqsvskerpe lalhinqnhq nqsepqlqql qiqvqlqhqq
      601 qqqqtapsph qqhqgllspg lsftgsglel dsgssvggsl pspgaascsl dtaststsps
      661 calmehapsp attvstasvq ptgpvgeppl tqrvgvlqqr lglpgdcqle fvngghgikn
      721 plaiegqrqa aanreeerat rpppgkdddp nrftcrvcsk nfslqrllnr hmkchsdvkr
      781 ylctfcgkgf ndtfdlkrht rthtgvrpyk cnlceksftq rcsleshclk vhgvqhqyay
      841 kerrtkvyvc eecghttqep evhylhlkdk hpyspallkf ydkrhfkftn snfanmllqg
      901 gllqrdsrna dscvksasks leaplqratt llhlgrassf
//
LOCUS       XP_034186337             938 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607151 isoform X2 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186337
VERSION     XP_034186337.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330446.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..938
                     /product="uncharacterized protein LOC117607151 isoform X2"
                     /calculated_mol_wt=100661
     Region          753..773
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(758,760,762,764..765,768..769,772,786,788,792..793,
                     796..797,800,814,816,818,820..821,824..825,829)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          766..789
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          781..>845
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          781..802
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          781..801
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(786,792,799,801,808,815)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          793..818
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          809..830
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          809..830
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..938
                     /gene="LOC117607151"
                     /coded_by="XM_034330446.2:592..3408"
                     /db_xref="GeneID:117607151"
ORIGIN      
        1 mpkifliknr lhqqqlrlle aqhlgksppp gsgrdspigs seplslivnk hqyrdktddd
       61 rattpeslrs tspasspppq wqststptst pprrfissil ggdvpygsrg hvltraerke
      121 yssppiapss sdasqfltks eklvlprpst ppktprvepp trvsviqrvp sqnqsasrre
      181 dkvempqtqe peqeqpidya vpkrkeedee rgregakvsr aignsiarpl lamrlsgpqv
      241 vhaaaghgrs sssgnngssg ssggsnnsgs ssssssggtg nycsggavtg gsggggavgg
      301 gaggggmnpg gnggrgnygp sspptgslpp fyeslkggnn lanfanqyns tqgngyltps
      361 ptvgmecdtg qqdvnsqhsq ynaqegkqys llqnvcasyg ltlkeeedls pykiqandll
      421 sgqystydmt dtgmmvdmvt gavvdplqft atltfsspsd htalleslgd aadlflprlp
      481 aedgsndlle eslhspasag sgigqdagqm ttpvepsvdp fpehsmaltr gfdtsrhyta
      541 apqhfstskl glayttgess yqsvskerpe lalhinqnhq nqsepqlqql qiqvqlqhqq
      601 qqqqtapsph qqhqgllspg lsftglelds gssvggslps pgaascsldt aststspsca
      661 lmehapspat tvstasvqpt gpvgeppltq rvgvlqqrlg lpgdcqlefv ngghgiknpl
      721 aiegqrqaaa nreeeratrp ppgkdddpnr ftcrvcsknf slqrllnrhm kchsdvkryl
      781 ctfcgkgfnd tfdlkrhtrt htgvrpykcn lceksftqrc sleshclkvh gvqhqyayke
      841 rrtkvyvcee cghttqepev hylhlkdkhp yspallkfyd krhfkftnsn fanmllqggl
      901 lqrdsrnads cvksasksle aplqrattll hlgrassf
//
LOCUS       XP_034186338             934 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607151 isoform X3 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186338
VERSION     XP_034186338.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330447.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..934
                     /product="uncharacterized protein LOC117607151 isoform X3"
                     /calculated_mol_wt=100190
     Region          749..769
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(754,756,758,760..761,764..765,768,782,784,788..789,
                     792..793,796,810,812,814,816..817,820..821,825)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          762..785
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          777..>841
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          777..798
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          777..797
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(782,788,795,797,804,811)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          789..814
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          805..826
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          805..826
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     CDS             1..934
                     /gene="LOC117607151"
                     /coded_by="XM_034330447.2:593..3397"
                     /db_xref="GeneID:117607151"
ORIGIN      
        1 mpkifliknr lhqqqlrlle aqhlgksppp gsgrdspigs seplslivnk hqyrdktddd
       61 rattpeslrs tspasspppq wqststptst pprrfissil ggdvpygsrg hvltraerke
      121 yssppiapss sdasqfltks eklvlprpst ppktprvepp trvsviqrvp sqnqsasrre
      181 dkvempqtqe peqeqpidya vpkrkeedee rgregakvsr aignsiarpl lamrlsgpqv
      241 vhaaaghgrs sssgnngssg ssggsnnsgs ssssssggtg nycsggavtg gsggggavgg
      301 gaggggmnpg gnggrgnygp sspptgslpp fyeslkggnn lanfanqyns tqgngyltps
      361 ptvgmecdtg qqdvnsqhsq ynaqegkqys llqnvcasyg ltlkeeedls pykiqandll
      421 sgqystydmt dtgmmvdmvt gavvdplqft atltfsspsd htalleslgd aadlflprlp
      481 aedgsndlle eslhspasag sgigqdagqm ttpvepsvdp fpehsmaltr gfdtsrhyta
      541 apqhfstskl glayttgess yqsvskerpe lalhinqnhq nqsepqlqql qiqvqlqhqq
      601 qqqqtapsph qqhqgllspg lsftgsgssv ggslpspgaa scsldtasts tspscalmeh
      661 apspattvst asvqptgpvg eppltqrvgv lqqrlglpgd cqlefvnggh giknplaieg
      721 qrqaaanree eratrpppgk dddpnrftcr vcsknfslqr llnrhmkchs dvkrylctfc
      781 gkgfndtfdl krhtrthtgv rpykcnlcek sftqrcsles hclkvhgvqh qyaykerrtk
      841 vyvceecght tqepevhylh lkdkhpyspa llkfydkrhf kftnsnfanm llqggllqrd
      901 srnadscvks asksleaplq rattllhlgr assf
//
LOCUS       XP_034186339             933 aa            linear   INV 03-SEP-2025
DEFINITION  uncharacterized protein LOC117607151 isoform X4 [Osmia lignaria
            lignaria].
ACCESSION   XP_034186339
VERSION     XP_034186339.1
DBLINK      BioProject: PRJNA1308557
DBSOURCE    REFSEQ: accession XM_034330448.2
KEYWORDS    RefSeq.
SOURCE      Osmia lignaria lignaria
  ORGANISM  Osmia lignaria lignaria
            Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
            Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita;
            Aculeata; Apoidea; Anthophila; Megachilidae; Megachilinae; Osmia.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_135045) annotated using gene prediction method: Gnomon.
            Also see:
                Documentation of NCBI's Annotation Process
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Full annotation
            Annotation Name             :: GCF_051020975.1-RS_2025_08
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.5
            Annotation Method           :: Gnomon; cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 08/27/2025
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Osmia lignaria lignaria"
                     /isolate="PbOS001"
                     /sub_species="lignaria"
                     /db_xref="taxon:1437193"
                     /chromosome="14"
                     /sex="male"
                     /tissue_type="Whole body"
                     /dev_stage="adult"
                     /lat_lon="40.27987392 N 75.29933094 W"
                     /collection_date="2022-04-11"
                     /collected_by="G. Gutierrez, P. Piz"
     Protein         1..933
                     /product="uncharacterized protein LOC117607151 isoform X4"
                     /calculated_mol_wt=100024
     Region          748..768
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(753,755,757,759..760,763..764,767,781,783,787..788,
                     791..792,795,809,811,813,815..816,819..820,824)
                     /site_type="other"
                     /note="putative nucleic acid binding site [nucleotide
                     binding]"
                     /db_xref="CDD:275368"
     Region          761..784
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          776..>840
                     /region_name="SUF4-like"
                     /note="N-terminal domain of Oryza sativa transcription
                     factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana
                     SUF4 (AtSUF4), and similar proteins; cd20908"
                     /db_xref="CDD:411020"
     Region          776..797
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          776..796
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:275368"
     Site            order(781,787,794,796,803,810)
                     /site_type="other"
                     /note="putative DNA binding site [nucleotide binding]"
                     /db_xref="CDD:411020"
     Region          788..813
                     /region_name="zf-H2C2_2"
                     /note="Zinc-finger double domain; pfam13465"
                     /db_xref="CDD:463886"
     Region          804..825
                     /region_name="C2H2 Zn finger"
                     /note="C2H2 Zn finger [structural motif]"
                     /db_xref="CDD:411020"
     Region          804..825
                     /region_name="C2H2 Zn finger"
             
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